LOCUS       DAZGXZ010000001       120830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000001 DAZGXZ010000000
VERSION     DAZGXZ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 120830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 120830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..120830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.001"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(119..814)
                     /gene="aqpZ"
                     /locus_tag="ACHKJF_000001"
     CDS             complement(119..814)
                     /gene="aqpZ"
                     /locus_tag="ACHKJF_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP0947431.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1240..2898
                     /gene="ybjD"
                     /locus_tag="ACHKJF_000002"
     CDS             1240..2898
                     /gene="ybjD"
                     /locus_tag="ACHKJF_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP0947432.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2895..3851)
                     /gene="ybjX"
                     /locus_tag="ACHKJF_000003"
     CDS             complement(2895..3851)
                     /gene="ybjX"
                     /locus_tag="ACHKJF_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP0947433.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            4002..5117
                     /gene="macA"
                     /locus_tag="ACHKJF_000004"
     CDS             4002..5117
                     /gene="macA"
                     /locus_tag="ACHKJF_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP0947434.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     DTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5114..7060
                     /gene="macB"
                     /locus_tag="ACHKJF_000005"
     CDS             5114..7060
                     /gene="macB"
                     /locus_tag="ACHKJF_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP0947435.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAETEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRNLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAR
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7133..7357)
                     /gene="cspD"
                     /locus_tag="ACHKJF_000006"
     CDS             complement(7133..7357)
                     /gene="cspD"
                     /locus_tag="ACHKJF_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP0947436.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7507..7581
                     /gene="yljB"
                     /locus_tag="ACHKJF_000007"
     CDS             7507..7581
                     /gene="yljB"
                     /locus_tag="ACHKJF_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP0947437.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7680..8000
                     /gene="clpS"
                     /locus_tag="ACHKJF_000008"
     CDS             7680..8000
                     /gene="clpS"
                     /locus_tag="ACHKJF_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP0947438.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            8031..10307
                     /gene="clpA"
                     /locus_tag="ACHKJF_000009"
     CDS             8031..10307
                     /gene="clpA"
                     /locus_tag="ACHKJF_000009"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP0947439.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10651..10738)
                     /locus_tag="ACHKJF_000010"
     tRNA            complement(10651..10738)
                     /locus_tag="ACHKJF_000010"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10702..10704),aa:Ser,seq:gga)
     gene            complement(10992..11210)
                     /gene="infA"
                     /locus_tag="ACHKJF_000011"
     CDS             complement(10992..11210)
                     /gene="infA"
                     /locus_tag="ACHKJF_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP0947440.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11495..12199)
                     /gene="aat"
                     /locus_tag="ACHKJF_000012"
     CDS             complement(11495..12199)
                     /gene="aat"
                     /locus_tag="ACHKJF_000012"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP0947441.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMSLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12241..13962)
                     /gene="cydC"
                     /locus_tag="ACHKJF_000013"
     CDS             complement(12241..13962)
                     /gene="cydC"
                     /locus_tag="ACHKJF_000013"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP0947442.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13963..15729)
                     /gene="cydD"
                     /locus_tag="ACHKJF_000014"
     CDS             complement(13963..15729)
                     /gene="cydD"
                     /locus_tag="ACHKJF_000014"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP0947443.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15852..16817)
                     /gene="trxB"
                     /locus_tag="ACHKJF_000015"
     CDS             complement(15852..16817)
                     /gene="trxB"
                     /locus_tag="ACHKJF_000015"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP0947444.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17362..17856
                     /gene="lrp"
                     /locus_tag="ACHKJF_000016"
     CDS             17362..17856
                     /gene="lrp"
                     /locus_tag="ACHKJF_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP0947445.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17991..22058
                     /gene="ftsK"
                     /locus_tag="ACHKJF_000017"
     CDS             17991..22058
                     /gene="ftsK"
                     /locus_tag="ACHKJF_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP0947446.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQQQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPRQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            22213..22824
                     /gene="lolA"
                     /locus_tag="ACHKJF_000018"
     CDS             22213..22824
                     /gene="lolA"
                     /locus_tag="ACHKJF_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP0947447.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FTAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22835..24178
                     /gene="rarA"
                     /locus_tag="ACHKJF_000019"
                     /gene_synonym="mgsA"
     CDS             22835..24178
                     /gene="rarA"
                     /locus_tag="ACHKJF_000019"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP0947448.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDRGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24269..25561
                     /gene="serS"
                     /locus_tag="ACHKJF_000020"
     CDS             24269..25561
                     /gene="serS"
                     /locus_tag="ACHKJF_000020"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP0947449.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(25801..25941)
                     /locus_tag="ACHKJF_000021"
     CDS             complement(25801..25941)
                     /locus_tag="ACHKJF_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0947450.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            26210..26407
                     /locus_tag="ACHKJF_000022"
     CDS             26210..26407
                     /locus_tag="ACHKJF_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947451.1"
                     /translation="MILSRKKIEIEVVQSGMADNPETITKIKVSRPEDLEACGVIVEK
                     ICAIHAKSVNTTASNDHNKYN"
     gene            complement(26523..26663)
                     /locus_tag="ACHKJF_000023"
     CDS             complement(26523..26663)
                     /locus_tag="ACHKJF_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0947452.1"
                     /translation="MPQKTIIVSILCITILMIVWMIHTSPCEFRLNIMWSQFAAFLQC
                     KQ"
     gene            complement(26820..27110)
                     /locus_tag="ACHKJF_000024"
     CDS             complement(26820..27110)
                     /locus_tag="ACHKJF_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947453.1"
                     /translation="MQGFAKVCKNSGFLRIPKNFNALNNKRFLKLRHDRRSEKGSGDE
                     NEKIRKARTGAAWRGVAAKITFAHFCTTAPKKDGEGEGKTATSSVARNGRKR"
     gene            complement(27526..29199)
                     /locus_tag="ACHKJF_000025"
     CDS             complement(27526..29199)
                     /locus_tag="ACHKJF_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947454.1"
                     /translation="MNPTKRLLLGNDTIQLVSCSVMLELNACGRGFITARTEQNYTGR
                     PVRLDIGYGNDLVRWFTGYVERSQPAENGSVRLLIREMAGILDHPFPCSFQHPTMRTV
                     TQWLSETSGLEIILPDNAAYTDRPVPHFTHSGTGYELLASLGRIFSVPDYIWHPLPDG
                     GIMAAAAAQGMFSGRPVTIPHEFSQASTGGHSMTLPMIQTLRPGVEVNGQRLTMVMLE
                     DDNMTITWTPRNKATGAPLQKSPFRRQAEKAFPELASGLHLPKLARVEAPTEAVSAGN
                     IADPFRPRYAVNLQLLDENGNPAADTPVYNAVPLPVPMAGSESGMFQFPPPGTLVEVG
                     FVEGRADKPFIRQIMAEGHNLPDVKPGEQLQQQRDGVSQRVTVAGDWERQTDQRIQET
                     SRERVVSADEEKRSLTSRETTIQATDTTTVLGVSTLQAGAVTHITEENYSISTGGHMM
                     VVAGTCERDVEGDTQDTVGGSLTEKIKGVRHSIALAQKLVGDTVKLGNEGINVLTMLT
                     DLADVVEELADITASHTHPKTGTSPQAAQFSQVAQECRQLKNKYSPIIE"
     gene            complement(29202..29744)
                     /locus_tag="ACHKJF_000026"
     CDS             complement(29202..29744)
                     /locus_tag="ACHKJF_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947455.1"
                     /translation="MSQTALLALDGEGIFMQNMVISPSMQFQEKDQSGQTSSTANAEQ
                     GIKAKELRVTGMITFDNERALQRIFQLASATTGDGALKVYRIANATAAAINFREGTFS
                     GQIDAQQQTDRLAWQVSFTLRERRSVPEKRQARAAPGSSRKQTPQTPGAKGGTVADET
                     PEKMTWFEEKVLKPVNDALE"
     gene            complement(29716..30441)
                     /locus_tag="ACHKJF_000027"
     CDS             complement(29716..30441)
                     /locus_tag="ACHKJF_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947456.1"
                     /translation="MTWTRKTISVSADMPPLSCSVIPVTPWTYGLGRYEESGVYLSPP
                     NAINWLAGKMAGSRASGDVTIIMIAENTHDLFIQSMAALTAVLPVPVFTQAQRMAQAA
                     ASLSTDKMQIPITTDTLPAPVHLSVATMRSAVSAARAAQARQLAAQRPDAAGLRRQIS
                     RFISRRAGALAGLEDGIRTLAEQKAQAWVFQYRGYHRAAAAAMVKDIPAPTAVHTVAA
                     LLAADSLTELGKMIHEPDRTARP"
     gene            complement(30453..31049)
                     /locus_tag="ACHKJF_000028"
     CDS             complement(30453..31049)
                     /locus_tag="ACHKJF_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0947457.1"
                     /translation="MTFKMSEHPQTIKIYNLSAGTNEFIGEGDAWIPPHTGLPAHSTD
                     IAPPDIPEGHVAIFDEDENAWHLMEDHRGKAVWRLESGSALLITEPGPLPENVTEIKP
                     DGEFCRWDGSSWVKDEAAEQAYRVSEAENKKRELIQEATNTINILQDAVDLNMATDDE
                     SALLPLWKKYRVLLNRTDTGNAPDIVWPESPSPDHHAG"
     gene            complement(31049..33109)
                     /locus_tag="ACHKJF_000029"
     CDS             complement(31049..33109)
                     /locus_tag="ACHKJF_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0947458.1"
                     /translation="MAKSVITSAFIRLKAQQAAEGTPVVLDEFVFANVPDLNPETPVS
                     PDETLPPEDQIVHRQAVGKTGVVNENAVVYSVTLGAELGDFEFNWVGLVSKATSTLAM
                     IVHAPTQKKIRTHNGNEGNVLVRSMLMEYSGAKEATGITTPAQTWQIDFTARLASMDE
                     RQRRENVDIYGKATFFDDGWLVKKGETWTVNKGVGYVEGLRAELSTHHPMTTPAFPSR
                     IWLNVWWTGTLTSEWATEHEIITADPDVTEERLRKTYPRDGIQQYFCAIAEIDEDGNV
                     TDLRPRGTPEERRSLDALKKHEKSRNHPDATLKEKGFTQLSSATTSTSETQAATPKAI
                     KIAMDNADGRLAKNRNGEDIPNKELFLINVGAPRVYAKDGYVGKAGQRWTTEQFVEWL
                     NERGAFRPGLWIFKANISLSQHSVLTDTGYGDICLGGCLIEVSGDINGGLIRITTGIE
                     SPEDITRPHRTQFTGVILSKGKSAIWYRTYTDKNKPTPQDIGALPASELLPAGVPLPW
                     PSDTPPAGYAIMVGQTFDKAQYPLLAKAYPDGRIPDMRGWTIKGKPASGRAVLSQEQD
                     GIKSHTHGGSVSNTDLGTKTTSSFNYGTKTTNSAGPFSATISLRTATASPEGSGDWTA
                     YGPGSGANLIARVSEHTHSVSIGEHTHTLALGQHGHGLTINPTGNAENTVKNIAFNYI
                     VRLA"
     gene            complement(33132..33680)
                     /locus_tag="ACHKJF_000030"
     CDS             complement(33132..33680)
                     /locus_tag="ACHKJF_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0947459.1"
                     /translation="MNDFLQRLRSLKLPSWMDRGEPDKLLRAAQAFWSRVWEWITWPV
                     RQFDPMTCAEPVLDLLAWERDITRFKGEPTSLYRRRIAHAFVNAQDAGEVAGFIAIFE
                     RLGIGHVELQERKPGVDWDVILVQVTDSQIADNSDLLLEIIRKYGRTCRRYRFEVETL
                     NPFIIHYGAYEGEYICWTARAE"
     gene            complement(33673..34857)
                     /locus_tag="ACHKJF_000031"
     CDS             complement(33673..34857)
                     /locus_tag="ACHKJF_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016507090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0947460.1"
                     /translation="MKGKPDVDFNQVFRESGMPETEEEIATLFHEDVQKTGLITNTSP
                     MSPFWRLILTIVIRPVQWLRDALVDVVLPGMFVATADRSLLLLHAHDVNITPKPASKA
                     SGVIWFAKTRANDDVLIPEGTIVQTARINGVIYSMAVTEETLISSGKSGASVPVEATT
                     EGSGHNLAPGYFRILPVAIPGIASVVNREDWLTVPGADEESDDELRDRIRNQFNLVSS
                     YHTDAVYRGLIASVAGLSIDRIYFQHDAPRGPGTANAYLLLDSGEASAPFIEAVNRYI
                     NEEGHHGHGDDLQCFAMPETRHRLEVTVYIRSSTTLPPDGAEGLKQDIGDLIRCAFRE
                     NARFNVKKVWPYSRFSFSTLGKEIHQTFPVVASVQFSLEDIISDLAVPRLESLTVEVV
                     IE"
     gene            complement(34835..35185)
                     /locus_tag="ACHKJF_000032"
     CDS             complement(34835..35185)
                     /locus_tag="ACHKJF_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2590 family protein"
                     /protein_id="HFP0947461.1"
                     /translation="MNTPSLYIDLLITDGSFTLNSGNEPQRCDNRVSIGQDVVHSIIE
                     SGIAALLIGERSPTLRSDIITRLILLVEDDERLVPGTVFINEEAPGRLHVTAETQDFG
                     RINTRVNYERETGR"
     gene            complement(35182..37368)
                     /locus_tag="ACHKJF_000033"
     CDS             complement(35182..37368)
                     /locus_tag="ACHKJF_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0947462.1"
                     /translation="MASQLDFIINLTDQLTRPLRQVQESVTGFAERSQGAFARIGVGV
                     AGLWGVAEGIRAAMDPAWEMQKALGELEAKGVGAQALTALTTQAMKLGTAFGQSSTEI
                     IRSAWHIRGALDGLSDGDLPRVTAATSTLAVAARVSSEEASGYLAAMGARFRSEFSAM
                     GAVGFAEDLAGKTAWMVQQFGVEMSTMQELIEGTKNAGADFGVSMAEQFAILGTLSRT
                     MGTEASGIYEEFLRSAPAAAQKMGMSFVDATGKILPMGDILDRLQARFGRNIEGNVAA
                     QQALDDAFGSGADVIKKLWGQQEMLNRSISALGRNDGMKRATEMAEAMTDPWDRLVAS
                     FYNIRAAIGNALYPILAPLAGRFADIGATFVRWLNMFPNIARWLGYITLTMLALGAAG
                     AVTNIVLGVFSFIMEGHKAIIKGARAVWSLYTLALTKVRSAIIATSLAARLAGVSFLT
                     MSAPVLLVAAAIAALVVVAIKFWQPVKAFIRGFIDGFTGALDAMSPVTAAFGEIARAL
                     APVWQGIKTLFGAFTDLFTPVQLTTGQMNSVTRAGETCGRVVAAAVSLISPALEVVIS
                     VIRTLVDIFAIVIRGWQDVVAAFDPTAPVESFRKMAGIIGNVFGDLWNVIKNSFNRTW
                     NWLAEKLNKIPGISIDMKPTEQVNTMTRGATGSTVPPQIAETAVPALAGNRISAELPP
                     GGIRNQIRTDTSTRIDNSRTFGNLTINTQNPMTPGQLDEWTELYAG"
     gene            37367..37594
                     /locus_tag="ACHKJF_000034"
     CDS             37367..37594
                     /locus_tag="ACHKJF_000034"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947463.1"
                     /translation="MSPSVKCDRYTFSGLVGAGTGKIFIQPDGTGDALFVVRLSGEVV
                     TPQCQILLKGIAADAFDPRGQFFNSISSFGE"
     gene            complement(37558..37833)
                     /locus_tag="ACHKJF_000035"
     CDS             complement(37558..37833)
                     /locus_tag="ACHKJF_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail assembly chaperone"
                     /protein_id="HFP0947464.1"
                     /translation="MTTKSTGTITLTVAGVTLTFEPNKTAFNNLINEITMTNKIAPMT
                     TYLNRIVTADCRDALADLMDRYPGCEMQIAEKINEVYSPKLEIELKN"
     gene            complement(37933..38319)
                     /locus_tag="ACHKJF_000036"
     CDS             complement(37933..38319)
                     /locus_tag="ACHKJF_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947465.1"
                     /translation="MMKQLAGCLVILFAAMWAGWLAHDWHDARVALAEQEAMEATRRA
                     VTEAGQQSARILEKRLEALKGNEIHTERVIHTETVRPVFRNVCATDDYVRLFNARAEA
                     AERTLAGKPADGMPEDTAETGRPDRR"
     gene            complement(38316..38654)
                     /locus_tag="ACHKJF_000037"
     CDS             complement(38316..38654)
                     /locus_tag="ACHKJF_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFP0947466.1"
                     /translation="MKLSEKQQLFAVMIADLIHWAQEHGYRLTFGEAYRTPEQAALNA
                     KSGKGIRNSLHTLRLAVDFNLFINGKYQADTDAYRPLGEYWESIGGTWGGRFSRADGN
                     HFSLEHNGVK"
     gene            complement(38654..39406)
                     /locus_tag="ACHKJF_000038"
     CDS             complement(38654..39406)
                     /locus_tag="ACHKJF_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0947467.1"
                     /translation="MTHKPYISPSLSLYHAQAHDVLRMLPENSVDALITDPPYSSGAT
                     HKAGRTCQGSHAKYLNDEDLRRFEGFEGENMDARSWGYWTHLWMTQAWRTVRPGGYAL
                     VFTDWRQLPALTDVFQAAGFTWRGIVAWNKGRGSRTPHTGYFRHQCEYVVWGSKGALG
                     KSPSGPFDGCITHHTRPQEKQHPTGKPESLMDELVRVCEPGGLILDPFMGSGTTGVAA
                     LRRGCRFTGIELNAHYFSVAQARLTGTEQRND"
     gene            complement(39396..39701)
                     /locus_tag="ACHKJF_000039"
     CDS             complement(39396..39701)
                     /locus_tag="ACHKJF_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HFP0947468.1"
                     /translation="MPDGQTSLLAKLIIIGAAIGLGRLMVSRDRITARLLIGRMIMGS
                     AVAPVAAIPLFKFPDMPELVVVGLACALGIMGSAFIEECFCRLADRYLKQKERRNDP"
     gene            complement(39706..40167)
                     /locus_tag="ACHKJF_000040"
     CDS             complement(39706..40167)
                     /locus_tag="ACHKJF_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein"
                     /protein_id="HFP0947469.1"
                     /translation="MAGNERISGGAFDINYDGIMIHVESATVTITDNSAVAQTRGVPN
                     GYTRGSVSADVEVEVDAQNFKKFGAVARSAGSWRAIPEKDFLFYANNGSEEEKIEVFG
                     CVPMLSDVVNISAGEAGKTTKKIKFMVTSPDFISIDGVPYLSARDTRDLKG"
     gene            complement(40169..41314)
                     /locus_tag="ACHKJF_000041"
     CDS             complement(40169..41314)
                     /locus_tag="ACHKJF_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2586 domain-containing protein"
                     /protein_id="HFP0947470.1"
                     /translation="MSWPNVTVSHQNRFNGATREVERTLLFVGYGKKNTGNTLSVSPE
                     TDLDDVLGPDESLLKSTLTAAIANGGQNWFAYVHVLSEPKPPAPEGGDANAAWVDAVK
                     KAQTIASAEGVVIAIDITARDAVNRATETRALLQSAYGRFVWFMLCVAGPGKDEAWAA
                     YVTRISAIQDGVAAPGVMVVPRLWGNEPGVLAGRLCNPSVTVADSPARVATGALVAMG
                     NDEIPQDGKKQPLELATLRSLEGKRFSVPMWYHDFDGLYWSDGRTLDVEGGDYQVIEN
                     VRVVDKVSRRVRLRAIARLADRALNSTPGSIAASQTYFSRTLREMALATEINGVPFPG
                     EVKPPQDGDITIVWTSSTHVQIYIVVRPYESAKAIGVSIELDTALEI"
     gene            complement(41326..42090)
                     /locus_tag="ACHKJF_000042"
     CDS             complement(41326..42090)
                     /locus_tag="ACHKJF_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0947471.1"
                     /translation="MISGQLNPQQLAEAQAMLQSLDLPPKKRQRLLWRIARHGIIAAA
                     RRHQRNQCAPDGTPWAPRKNKKKKAGKNTGKMLKKLPRLMAVREIPGQDAVKVYFRAV
                     RYGPTASASNVAWAHHNGATIRYHGDSFRHKKPARSVPCSLKQAQALRAWGLELKSGK
                     RWRKATLSMIRRRVSNRQAGWLLARFERDDWEDFRDLYSSDGQGIRQRNNPRTWTITL
                     PSRVFLGVTNEEFSHILARQMNAMGYGLDVDAQDIR"
     gene            complement(42087..42560)
                     /locus_tag="ACHKJF_000043"
     CDS             complement(42087..42560)
                     /locus_tag="ACHKJF_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0947472.1"
                     /translation="MNQLESLTAFITENLPSDAMQFFMSSQEDAQLIRSQKALGNDQI
                     RMGVMTYTGVIAWDNFPFRKYSPGLVYALVMAWLEEHADPLREEFCPEDVNVDPQFND
                     EDSCLLDITVGLADPLIIRVQEDGPVPYRGKRWTLVNPEIWTAETMDLFVSRSRE"
     gene            complement(42557..43030)
                     /locus_tag="ACHKJF_000044"
     CDS             complement(42557..43030)
                     /locus_tag="ACHKJF_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0947473.1"
                     /translation="MSEPSFSVSGKPLKSTPQTVTNGEPFWPDLDLAELQGSRTLPAD
                     LPDDTAAMALLAAVAEVNTTLAPVVVHWKAQGHERAADVPGARLGDETQLVAQYKKAV
                     YARAKADLMGEFATIGRRETYPGQESEDTRATLLAEASLVMRNMLGHRRAGVHII"
     gene            complement(43138..43836)
                     /gene="gpM"
                     /locus_tag="ACHKJF_000045"
     CDS             complement(43138..43836)
                     /gene="gpM"
                     /locus_tag="ACHKJF_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP0947474.1"
                     /translation="MTPCQRHRAKIRTQEALERREALTASPVSFHLLRAELDRDVARL
                     RSLPVREERLAFKRDILLPRWLPVAERYIADGKRHACPVLVYCIIWLFDTGDLSRALD
                     WADIAISEGQATPDNFRSTLPAFVADTVLQWATDNSEAGHSIEPYFSRTFRNVTTTWR
                     LHEEIRAKWFRFAGLYLLRDEAGKPRATAINDTAVLEKADAYLAQAAALSRTAGVKSI
                     RARIRARISALNAA"
     gene            complement(43854..44882)
                     /locus_tag="ACHKJF_000046"
     CDS             complement(43854..44882)
                     /locus_tag="ACHKJF_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0947475.1"
                     /translation="MQLNQKTESLLRKYAAGLGAANGVDDVTRYFALSDPKETALRDA
                     IMHSDEFLSKLPNVLDVEQVTGQVVTTGVPGLHTGRKKNARFTLPLGVTGNEYKLAET
                     DSGAHLLYTTLVTWANAGGENEFFNRIQNFVNRQIALDILRIAFNGTSVAEDTDPATN
                     KNGEDVNTGWHAIVKGRSEKQIVEAGPTLGKGGDFIGLDAAVADLVHTLIDESYRGHP
                     DLVVLVAPDLVAQDKVTLLNAIDAPTEKVAAQLIGRQIAGLTAYTPPFMPEGRLIVTT
                     LANLHIYTQRGTRQRRAEWNDDRKRFENNYLRMEGYAVEHDEIYAAYDKLTIKGAEAA
                     EEPASGGA"
     gene            complement(44894..45943)
                     /locus_tag="ACHKJF_000047"
     CDS             complement(44894..45943)
                     /locus_tag="ACHKJF_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0947476.1"
                     /translation="MPQPTLMTDWICIATSGPTVDGREIDPQWLTDAAETYSRNTYTA
                     LLWPWHEEEIGHRQYTSNLGEVDSLKAETGEDGKTRLYARLVPNQYLIEANRLGQKLF
                     TSAEIVENFARSGRDYLLGVAVTDIPASLGTEKIRFTLNNEEYTAQRANCQTFTLGSL
                     SPDTSPAPRKPESFLKRLFSAGQTDNTDTSKPDTGEDAKMDEIKALLEKLITLVEGGS
                     AAATGEGTADTPEEAKAEVAEIAAEIADTAAEVAELAEEVEAAPEDEVKAEAFSAAKA
                     KLEDAIKAFSAITPEKGERYRRFAARRRDDTDKEEIKAIAEKLNDLTTKLSATGITPR
                     PAGAPAESEKPFDFV"
     gene            46110..47915
                     /locus_tag="ACHKJF_000048"
     CDS             46110..47915
                     /locus_tag="ACHKJF_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0947477.1"
                     /translation="MAKYSDEIKEAARSLYLKRWHPRDIAAELGIPPRTVYHWCDVGQ
                     WATLLPAESVEDVIARRIDVLTGREGKTECELAELRELIAQHVKIIAQKNKHAERMAE
                     IAALRPAGYEDGAAAGTCGGGKGKKRRYRKNDVSGLTAEMLDEWARKNLYAYQLHCRE
                     NRHRERRFILKSRQIGMTWYFAWEAFEDAVVTGDNQIFFSASRVQAEIFREYIINFAL
                     QFGITLTGKHIRLSNGAMLRFLSTNASTAQGFNGHLYGDEVFWIPKFTRLHEVASAMA
                     THDKYRTTYFSTPSAKTHQAYLIWNGDDWRGDDPARRAVEFPKESAMRVGCECPDGIW
                     RYIIRLEEAVAGGLSARVDIERIRNLYNPTTYAMLYGCEFVDSKDAVFKFSELVRCEV
                     EMETWGDYDPTAARPFGNREVWAGFDPSRSGDNSTFVIVAPPVHEGERFRVLAVWQWQ
                     GFNFTWQADQIRELMQRFNITYIGIDITGIGKGVFDIVSRFAPREANAILYSVESKNR
                     LVMKMIDVVAHKRIEWAKDAIDEASRERTEIPASFMSIRRTTTKSGNALTFVAERSDV
                     TGHADVFFAISHAVINEPVDYDYERPSTWAFGKAA"
     gene            47912..48964
                     /locus_tag="ACHKJF_000049"
     CDS             47912..48964
                     /locus_tag="ACHKJF_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0947478.1"
                     /translation="MSKKRRNKTSRVTKKAFTPARGSTITFGQPEPVLTTGTDYHNIW
                     YDNEHNHWQLPINRLALAQLPNLNAQHGGVLYARRNMVAGGYTGGGLTTDQMEQAVFD
                     YLLFGDLALLKVRNVFGDVVALEPLPSLYLRRRKTGEFVVLQEGPALVYTPEDIIFLR
                     MYDPRQQIYGLPDYIGGIHSALLNSEASLFRRRYYNNGAHMGFLLYTNDPGITTEMED
                     EIREKVAESKGLGNFRNLYINIPKGSPDGVKIIPIGEVSAKDEFSNIKGISAQDVFTA
                     HRFPAGLAGIIPTNGATIPNPETTRETYRKDEVIPLQRKIMNAVNSDPDIPEHLYLRF
                     DVEAGHVAEEKTVPVG"
     gene            49002..49265
                     /locus_tag="ACHKJF_000050"
     CDS             49002..49265
                     /locus_tag="ACHKJF_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0947479.1"
                     /translation="MRIFKARCPDCGAPAVIRKTDWKTPELADLYCACSDVECGHTFV
                     CTMTFSHTLSPGARSGRNMVKFLVDAMRPEDRRYALELLSRRS"
     gene            complement(49331..49657)
                     /locus_tag="ACHKJF_000051"
     CDS             complement(49331..49657)
                     /locus_tag="ACHKJF_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0947480.1"
                     /translation="MKTPGMTGKEIILRYLETHTRFSAPELVEKLGVKRTVINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERQELAQRAERQQEKSVIEACKRSEIMKRILFI
                     YGAAFR"
     gene            complement(49654..49863)
                     /locus_tag="ACHKJF_000052"
     CDS             complement(49654..49863)
                     /locus_tag="ACHKJF_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947481.1"
                     /translation="MNITRNSQLKERTMNSTRRISDRELTKMIESAQQLLSRQNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
     gene            complement(49873..50277)
                     /locus_tag="ACHKJF_000053"
     CDS             complement(49873..50277)
                     /locus_tag="ACHKJF_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947482.1"
                     /translation="MTRFYCAMQRQRFDEMMRQRTPLLTLNNIATGPQPGDELEITVV
                     SPDRTTTAIITDILNGRPDSTNRSWLVAVRPRRPPGEPEPLTPAGKIMRQYHEEQAHK
                     HNAELTRTLASINRKAAIARYVHSAAGRQSRR"
     gene            complement(50274..52793)
                     /locus_tag="ACHKJF_000054"
     CDS             complement(50274..52793)
                     /locus_tag="ACHKJF_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0947483.1"
                     /translation="MNDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVAFLTTTDEGKRINSHLHHDEERRIRLEKLTRHKRENPPTLVSEAMAE
                     LRALPPFLAGPLIRDLARLSRKQESARREGLKNNSAYAADNFVRHGLRARLKRIARVN
                     ERFATPAFKATAARERLDELLMLPQLSRDEIQHLATLTATAFSAALERACDEVIEKTG
                     RGDDDLFTWLLTYQPLAHMALKLGITPPCWPSLEIRPDRRTAPDPEQVPGAVMRLSCA
                     TWWNSQLRHLRDIWREELLRAAGLVSRKTSVYISHEALLDWREKRQRTRDFLKAWDIE
                     NEEGERFSLEDIYWAGLSNPRNRRNEMMACVRGMEQVAEARGDVAFFVTVTAPSRFHS
                     VNDDGSLNPKYCGATVRDASDYLVYRFFAAVRRTIDKAGLGWYGVRTVEPHHDGTPHW
                     HMLVFTSQENEARITEIMRNAAIREDRAELGDDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASAFMGNDPKTGKPYVDKESGKTMAETVENAIGWAALHRIRQFQFFGIPPRQV
                     WRELRRLAGQMARNPAAPQRLDHDDIDAILAAADVGCFATYITRQGGVLIPRNTYLVR
                     TAYETAEEANDYGEFPQRIYGVRAPSLGERYTICTHPDTWKLVRRKPENEDRTDEGFD
                     VECGFAAPWTRGNNCPPDQNLTINDPPERQPDGRREQRLTLPEGDLGEWLRSLTPTER
                     KQLTRQLKNPPPDTETVKNGGISAPAGGVRVSVALTAEPHITAQITRELDALGVQLHE
                     SAVVSVRHGARVRLADGRTAYWNDSTRRIDITPAPNTEAPQ"
     gene            complement(52790..53206)
                     /locus_tag="ACHKJF_000055"
     CDS             complement(52790..53206)
                     /locus_tag="ACHKJF_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947484.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPRLASRLNDWHDYYRRACKQAAR
                     RVTTYGDFSFTVDGRYRVDVKGDGDGFGLFHINGTDSTAISWSSSEMTLVLEMTNHSV
                     AVKGIYTAEDFIGEYQRLLVLYRAAFQAATAGECDA"
     gene            complement(53275..53502)
                     /locus_tag="ACHKJF_000056"
     CDS             complement(53275..53502)
                     /locus_tag="ACHKJF_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0947485.1"
                     /translation="MANRRQQRARAERLHIRTEINRRLWRASRVAQIMYINMLHEHAH
                     ALSDRYCAAVFSYLADDLAELQDLINQQHHH"
     gene            complement(53825..54124)
                     /locus_tag="ACHKJF_000057"
     CDS             complement(53825..54124)
                     /locus_tag="ACHKJF_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947486.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTPLAIRLTLRHEIEDFCATLEAPGEPETPEAIQKELLQRIDKVFDFFLNH"
     gene            complement(54121..54387)
                     /locus_tag="ACHKJF_000058"
     CDS             complement(54121..54387)
                     /locus_tag="ACHKJF_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0947487.1"
                     /translation="MTILDYIAANPGCSGSEIATALNTQTTAINAELRRLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEARA"
     gene            complement(54413..54625)
                     /locus_tag="ACHKJF_000059"
     CDS             complement(54413..54625)
                     /locus_tag="ACHKJF_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947488.1"
                     /translation="MISKYTTNARKPGKDARHHIAVLRNLLLEEIDDFTATLSGPGAP
                     VTPGEIHRDLKRRIENAIDYTLNPTE"
     gene            complement(54622..54873)
                     /locus_tag="ACHKJF_000060"
     CDS             complement(54622..54873)
                     /locus_tag="ACHKJF_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947489.1"
                     /translation="MNSIFTATRRTLLTHFTDAAGREFIVENYLITTTTPCPSDADYL
                     YIHLADGTQITAIASTVREVMTIRGAWKSETQAHGELRP"
     gene            complement(54885..55163)
                     /locus_tag="ACHKJF_000061"
     CDS             complement(54885..55163)
                     /locus_tag="ACHKJF_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947490.1"
                     /translation="MSINTPTEINKLFTDPAHIEFTQKTLTEITNVLDERISEIDKDK
                     HFATYMALQMQSMAMVTAKQTISELYMKNLRLERELAELRAQSGQFSA"
     gene            complement(55243..55368)
                     /locus_tag="ACHKJF_000062"
     CDS             complement(55243..55368)
                     /locus_tag="ACHKJF_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947491.1"
                     /translation="MSVFLSWVILIISVLMAIGIMRIIEALKNIESLFTGEERRK"
     gene            complement(55365..55607)
                     /locus_tag="ACHKJF_000063"
     CDS             complement(55365..55607)
                     /locus_tag="ACHKJF_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0947492.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTRDEGLRNSLRGAIMGLYN
                     CALGSDDREALEELKVIVAELFEGEIVEPYFTEVAA"
     gene            complement(55627..55965)
                     /locus_tag="ACHKJF_000064"
     CDS             complement(55627..55965)
                     /locus_tag="ACHKJF_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0947493.1"
                     /translation="MTQRRSFRTGTERHAARFSTSASRSNSYYSLQETHATPDGHLVK
                     QTGKHTWLINDAGIVIHRGARNPITGNTCYSLTRGDEQFGQDFTLHEALRTADRLISG
                     RSFQHPEHRR"
     gene            56074..56397
                     /locus_tag="ACHKJF_000065"
     CDS             56074..56397
                     /locus_tag="ACHKJF_000065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947494.1"
                     /translation="MIDKSVKDAITEYLVSIYTTNLAVAPVDKLTDPRDSVGVVSDLL
                     ISLLISELPDDKRDALIKTASEILRQIEGQQAFAPYAVNIRTRFHSINHLACFIRASK
                     DKPDL"
     gene            complement(56375..56893)
                     /locus_tag="ACHKJF_000066"
     CDS             complement(56375..56893)
                     /locus_tag="ACHKJF_000066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947495.1"
                     /translation="MTITPEEEGMHKKYSEHGSKAGEVIAIDEDCLITGDPWLATERD
                     LEGKTFFVRAIKHKDRVVGGIIYSTSTIIPDNYLDISFRPVSIQGCEAYLNYVAELKE
                     INLDYMWPIVQREIMRRKSEGFNVKIEFLSTLMGFLQKPLILFPSLDRNLPVKEGVEK
                     YIVEQVRDLVCL"
     gene            complement(56914..57309)
                     /locus_tag="ACHKJF_000067"
     CDS             complement(56914..57309)
                     /locus_tag="ACHKJF_000067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947496.1"
                     /translation="MSGKNKSEKNATVLKQLSLLKEEHKDATILMHLEGSPKITITTT
                     YSELHKELEGLINFCSKLKANENIDDLILKPEAEFGHFCESRENGCIGIMLYATRGIM
                     INLPVEQAVDIFGEMALNKTTIYKSKTTE"
     gene            complement(57316..57840)
                     /locus_tag="ACHKJF_000068"
     CDS             complement(57316..57840)
                     /locus_tag="ACHKJF_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFP0947497.1"
                     /translation="MRDFIADKELSDTDHTTGAAYINADTSQQEATDNNDEQKPQRTY
                     KKGATLRLDGPIAGLCSLEKGAAYIGLTKTALRAALQRNQMPGHKTRSNPEDENSDGV
                     WWFNVREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFTPADKEDCQTVNGKRVYKG
                     RKSKLEQSQDVNER"
     gene            57942..58289
                     /locus_tag="ACHKJF_000069"
     CDS             57942..58289
                     /locus_tag="ACHKJF_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0947498.1"
                     /translation="MNSTLSISIGEKILMIRKSERLTRKQFFELTGIPVNTQRYYETG
                     RQDGIGSEILLKITKNLRFKKYALWLTTDETFEAGGQIAPALSLDGSSQSEGDLASID
                     VTQKSSRSGRNAG"
     gene            58359..59351
                     /locus_tag="ACHKJF_000070"
     CDS             58359..59351
                     /locus_tag="ACHKJF_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0947499.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIEAWWLLDGRNQDYGDTYRVRLEKINREMGDPRASQMT
                     RKFMLGYRSDKLQAGLMPSSINRDLCALSAMFSSLIEGEVFHNENPVRGIRKLKVRNT
                     EMAFLSDEEIERLLERLDGDARRVAILCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDAVLALIKTRRTGLLYQADYLRFREILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLAESVHKLSID"
     gene            complement(59535..60419)
                     /gene="xapR"
                     /locus_tag="ACHKJF_000071"
     CDS             complement(59535..60419)
                     /gene="xapR"
                     /locus_tag="ACHKJF_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP0947500.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQLV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(60460..60618)
                     /locus_tag="ACHKJF_000072"
     CDS             complement(60460..60618)
                     /locus_tag="ACHKJF_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFP0947501.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(60671..61927)
                     /gene="xapB"
                     /locus_tag="ACHKJF_000073"
     CDS             complement(60671..61927)
                     /gene="xapB"
                     /locus_tag="ACHKJF_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP0947502.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(61987..62820)
                     /gene="xapA"
                     /locus_tag="ACHKJF_000074"
     CDS             complement(61987..62820)
                     /gene="xapA"
                     /locus_tag="ACHKJF_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP0947503.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHVQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            63069..63833
                     /gene="yfeN"
                     /locus_tag="ACHKJF_000075"
     CDS             63069..63833
                     /gene="yfeN"
                     /locus_tag="ACHKJF_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP0947504.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(63872..64798)
                     /gene="yfeR"
                     /locus_tag="ACHKJF_000076"
     CDS             complement(63872..64798)
                     /gene="yfeR"
                     /locus_tag="ACHKJF_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0947505.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            64888..65886
                     /gene="yfeH"
                     /locus_tag="ACHKJF_000077"
     CDS             64888..65886
                     /gene="yfeH"
                     /locus_tag="ACHKJF_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP0947506.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(65883..66101)
                     /gene="ypeB"
                     /locus_tag="ACHKJF_000078"
     CDS             complement(65883..66101)
                     /gene="ypeB"
                     /locus_tag="ACHKJF_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP0947507.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(66103..68118)
                     /gene="ligA"
                     /locus_tag="ACHKJF_000079"
     CDS             complement(66103..68118)
                     /gene="ligA"
                     /locus_tag="ACHKJF_000079"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP0947508.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPLAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(68189..69175)
                     /gene="zipA"
                     /locus_tag="ACHKJF_000080"
     CDS             complement(68189..69175)
                     /gene="zipA"
                     /locus_tag="ACHKJF_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP0947509.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            69405..70166
                     /gene="cysZ"
                     /locus_tag="ACHKJF_000081"
     CDS             69405..70166
                     /gene="cysZ"
                     /locus_tag="ACHKJF_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP0947510.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            70351..71322
                     /gene="cysK"
                     /locus_tag="ACHKJF_000082"
     CDS             70351..71322
                     /gene="cysK"
                     /locus_tag="ACHKJF_000082"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP0947511.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            71706..71963
                     /gene="ptsH"
                     /locus_tag="ACHKJF_000083"
     CDS             71706..71963
                     /gene="ptsH"
                     /locus_tag="ACHKJF_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP0947512.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            72008..73735
                     /gene="ptsI"
                     /locus_tag="ACHKJF_000084"
     CDS             72008..73735
                     /gene="ptsI"
                     /locus_tag="ACHKJF_000084"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP0947513.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            73776..74285
                     /gene="crr"
                     /locus_tag="ACHKJF_000085"
     CDS             73776..74285
                     /gene="crr"
                     /locus_tag="ACHKJF_000085"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP0947514.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(74327..75178)
                     /gene="pdxK"
                     /locus_tag="ACHKJF_000086"
                     /gene_synonym="thiJ"
     CDS             complement(74327..75178)
                     /gene="pdxK"
                     /locus_tag="ACHKJF_000086"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP0947515.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            75283..75651
                     /gene="yfeK"
                     /locus_tag="ACHKJF_000087"
     CDS             75283..75651
                     /gene="yfeK"
                     /locus_tag="ACHKJF_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP0947516.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(75654..76565)
                     /gene="cysM"
                     /locus_tag="ACHKJF_000088"
     CDS             complement(75654..76565)
                     /gene="cysM"
                     /locus_tag="ACHKJF_000088"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP0947517.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(76699..77796)
                     /gene="cysA"
                     /locus_tag="ACHKJF_000089"
     CDS             complement(76699..77796)
                     /gene="cysA"
                     /locus_tag="ACHKJF_000089"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP0947518.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(77786..78661)
                     /gene="cysW"
                     /locus_tag="ACHKJF_000090"
     CDS             complement(77786..78661)
                     /gene="cysW"
                     /locus_tag="ACHKJF_000090"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP0947519.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(78661..79494)
                     /gene="cysT"
                     /locus_tag="ACHKJF_000091"
     CDS             complement(78661..79494)
                     /gene="cysT"
                     /locus_tag="ACHKJF_000091"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP0947520.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLILLFSINTLQSRFGRRVVG
                     H"
     gene            complement(79494..80510)
                     /gene="cysP"
                     /locus_tag="ACHKJF_000092"
     CDS             complement(79494..80510)
                     /gene="cysP"
                     /locus_tag="ACHKJF_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP0947521.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(80681..81472)
                     /gene="ucpA"
                     /locus_tag="ACHKJF_000093"
     CDS             complement(80681..81472)
                     /gene="ucpA"
                     /locus_tag="ACHKJF_000093"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP0947522.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(81601..82458)
                     /gene="murR"
                     /locus_tag="ACHKJF_000094"
     CDS             complement(81601..82458)
                     /gene="murR"
                     /locus_tag="ACHKJF_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP0947523.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            82622..83518
                     /gene="murQ"
                     /locus_tag="ACHKJF_000095"
     CDS             82622..83518
                     /gene="murQ"
                     /locus_tag="ACHKJF_000095"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP0947524.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            83522..84946
                     /gene="murP"
                     /locus_tag="ACHKJF_000096"
     CDS             83522..84946
                     /gene="murP"
                     /locus_tag="ACHKJF_000096"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP0947525.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGCWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            84951..86255
                     /gene="pbp4b"
                     /locus_tag="ACHKJF_000097"
     CDS             84951..86255
                     /gene="pbp4b"
                     /locus_tag="ACHKJF_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP0947526.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(86313..87212)
                     /gene="yfeX"
                     /locus_tag="ACHKJF_000098"
     CDS             complement(86313..87212)
                     /gene="yfeX"
                     /locus_tag="ACHKJF_000098"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP0947527.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(87308..87883)
                     /gene="yfeY"
                     /locus_tag="ACHKJF_000099"
     CDS             complement(87308..87883)
                     /gene="yfeY"
                     /locus_tag="ACHKJF_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP0947528.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(87944..88393)
                     /gene="yfeZ"
                     /locus_tag="ACHKJF_000100"
     CDS             complement(87944..88393)
                     /gene="yfeZ"
                     /locus_tag="ACHKJF_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP0947529.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            complement(88380..88805)
                     /gene="ypeA"
                     /locus_tag="ACHKJF_000101"
     CDS             complement(88380..88805)
                     /gene="ypeA"
                     /locus_tag="ACHKJF_000101"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP0947530.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            89019..89888
                     /gene="amiA"
                     /locus_tag="ACHKJF_000102"
     CDS             89019..89888
                     /gene="amiA"
                     /locus_tag="ACHKJF_000102"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP0947531.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            89892..90791
                     /gene="hemF"
                     /locus_tag="ACHKJF_000103"
     CDS             89892..90791
                     /gene="hemF"
                     /locus_tag="ACHKJF_000103"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP0947532.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQRKAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(90797..91849)
                     /gene="eutR"
                     /locus_tag="ACHKJF_000104"
     CDS             complement(90797..91849)
                     /gene="eutR"
                     /locus_tag="ACHKJF_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP0947533.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(91895..92395)
                     /gene="eutK"
                     /locus_tag="ACHKJF_000105"
     CDS             complement(91895..92395)
                     /gene="eutK"
                     /locus_tag="ACHKJF_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP0947534.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(92408..93067)
                     /gene="eutL"
                     /locus_tag="ACHKJF_000106"
     CDS             complement(92408..93067)
                     /gene="eutL"
                     /locus_tag="ACHKJF_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP0947535.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(93077..93964)
                     /gene="eutC"
                     /locus_tag="ACHKJF_000107"
     CDS             complement(93077..93964)
                     /gene="eutC"
                     /locus_tag="ACHKJF_000107"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP0947536.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(93985..95346)
                     /gene="eutB"
                     /locus_tag="ACHKJF_000108"
     CDS             complement(93985..95346)
                     /gene="eutB"
                     /locus_tag="ACHKJF_000108"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP0947537.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(95358..96761)
                     /gene="eutA"
                     /locus_tag="ACHKJF_000109"
     CDS             complement(95358..96761)
                     /gene="eutA"
                     /locus_tag="ACHKJF_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP0947538.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLEEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(96758..97984)
                     /gene="eutH"
                     /locus_tag="ACHKJF_000110"
     CDS             complement(96758..97984)
                     /gene="eutH"
                     /locus_tag="ACHKJF_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP0947539.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKDDATAAKTEAEAQS"
     gene            complement(98101..99288)
                     /gene="eutG"
                     /locus_tag="ACHKJF_000111"
     CDS             complement(98101..99288)
                     /gene="eutG"
                     /locus_tag="ACHKJF_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP0947540.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(99278..100114)
                     /gene="eutJ"
                     /locus_tag="ACHKJF_000112"
     CDS             complement(99278..100114)
                     /gene="eutJ"
                     /locus_tag="ACHKJF_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP0947541.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(100125..101528)
                     /gene="eutE"
                     /locus_tag="ACHKJF_000113"
     CDS             complement(100125..101528)
                     /gene="eutE"
                     /locus_tag="ACHKJF_000113"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP0947542.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(101540..101827)
                     /gene="eutN"
                     /locus_tag="ACHKJF_000114"
     CDS             complement(101540..101827)
                     /gene="eutN"
                     /locus_tag="ACHKJF_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP0947543.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(101934..102227)
                     /gene="eutM"
                     /locus_tag="ACHKJF_000115"
     CDS             complement(101934..102227)
                     /gene="eutM"
                     /locus_tag="ACHKJF_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP0947544.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(102266..103282)
                     /gene="pta"
                     /locus_tag="ACHKJF_000116"
     CDS             complement(102266..103282)
                     /gene="pta"
                     /locus_tag="ACHKJF_000116"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0947545.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            complement(103279..104082)
                     /gene="eutT"
                     /locus_tag="ACHKJF_000117"
     CDS             complement(103279..104082)
                     /gene="eutT"
                     /locus_tag="ACHKJF_000117"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP0947546.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(104079..104780)
                     /gene="eutQ"
                     /locus_tag="ACHKJF_000118"
     CDS             complement(104079..104780)
                     /gene="eutQ"
                     /locus_tag="ACHKJF_000118"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP0947547.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(104755..105234)
                     /gene="eutP"
                     /locus_tag="ACHKJF_000119"
     CDS             complement(104755..105234)
                     /gene="eutP"
                     /locus_tag="ACHKJF_000119"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP0947548.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(105247..105582)
                     /gene="eutS"
                     /locus_tag="ACHKJF_000120"
     CDS             complement(105247..105582)
                     /gene="eutS"
                     /locus_tag="ACHKJF_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP0947549.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(105875..108154)
                     /gene="maeB"
                     /locus_tag="ACHKJF_000121"
     CDS             complement(105875..108154)
                     /gene="maeB"
                     /locus_tag="ACHKJF_000121"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP0947550.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            108443..109393
                     /gene="tal"
                     /locus_tag="ACHKJF_000122"
     CDS             108443..109393
                     /gene="tal"
                     /locus_tag="ACHKJF_000122"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0947551.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            109413..111416
                     /gene="tkt"
                     /locus_tag="ACHKJF_000123"
     CDS             109413..111416
                     /gene="tkt"
                     /locus_tag="ACHKJF_000123"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0947552.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(111493..112535)
                     /gene="ypfG"
                     /locus_tag="ACHKJF_000124"
                     /pseudo
     CDS             complement(111493..112535)
                     /gene="ypfG"
                     /locus_tag="ACHKJF_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
     gene            complement(112661..113236)
                     /gene="nudK"
                     /locus_tag="ACHKJF_000125"
     CDS             complement(112661..113236)
                     /gene="nudK"
                     /locus_tag="ACHKJF_000125"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP0947553.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEVI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(113304..115283)
                     /gene="aegA"
                     /locus_tag="ACHKJF_000126"
     CDS             complement(113304..115283)
                     /gene="aegA"
                     /locus_tag="ACHKJF_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP0947554.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRHPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            115489..117189
                     /gene="narQ"
                     /locus_tag="ACHKJF_000127"
     CDS             115489..117189
                     /gene="narQ"
                     /locus_tag="ACHKJF_000127"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP0947555.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            117353..120466
                     /gene="acrD"
                     /locus_tag="ACHKJF_000128"
     CDS             117353..120466
                     /gene="acrD"
                     /locus_tag="ACHKJF_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP0947556.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(120565..120624)
                     /gene="ypfM"
                     /locus_tag="ACHKJF_000129"
     CDS             complement(120565..120624)
                     /gene="ypfM"
                     /locus_tag="ACHKJF_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP0947557.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        27360 a        32558 c        32146 g        28766 t
ORIGIN      
        1 tccatgtggg ctgccccggc cttcggggaa cgcttcagcg attttgacgc caccaacacc
       61 cgtgcggttt gtaacgtcga agataaaaca accagggcag ataaaatgcc aatgctaatt
      121 aattacgttt ttccagcagg gtccggtaaa tcagaccacc gataatgccg ccgacaattg
      181 gcaccaccca gaagaaccac agttgttcta atgcccagcc gccctggaag atagcaaccg
      241 cggtgctgcg cgccgggtta acagaagtgt tagtcaccgg aatactaatt aagtgaatca
      301 gggttaaggc cagaccaata gcgatcggcg caaaacctgc cggcgcgaat ttgtcggttg
      361 cgccgtggat caccaacagg aaacctgcac tcaataccag ttcaactacc agcgcggaaa
      421 gcatggaata accgcctggt gaatgctcgc cataaccgtt agaagcaaaa ccgctggctg
      481 ccgcgtcaaa acccgtttta ccactggcaa ttaaatacag cagcgccgct gcaacaatac
      541 cgccgacaac ctgggcaatt acgtagccaa cgacttcttt tgccggaaaa cgtccgccag
      601 cccataaacc aatagtgacc gccgggttaa aatgaccacc agaaatatga ccaacagcaa
      661 aggccatcgt cagaacggtc agaccgaacg ccaacgccac gccggcaaaa ccaatgccta
      721 attccgggaa gcctgcggcc agtacagcac taccacagcc accaaaaaca agccagaaag
      781 taccaaaaca ttcagctgct aattttctga acatatccac ctcaattaaa aattgaccct
      841 gtgaaaaata tggtcgtttt atagggtcgt cgtaaaaaag tgacgacgga aataatgcgc
      901 ggctatttta aaaacgaagg cgagtcattc accagataaa taaatccagt aaatttgatt
      961 tagggcaaca gcgggttgcc ccatatagtc atttgtctga ttgacagtgt agtgcaagca
     1021 aaagatttaa tcctttaggc gtaataaaaa ataatttatc atgctaatta tttgattttg
     1081 ttgtttttgc agacttatca gcaagaggga gtataacgcg attattcgct catttttcag
     1141 acatttgcca tgcttaaatg tgatgtcatc acgtattagc aaggcctttc ccgttatact
     1201 gccagcgtaa aggataagtc acatatttct ggaggggata tgattcttga gcgcgttgaa
     1261 attgtgggtt ttcgcggtat caaccgtttg tcgttgatgc tggaacaaaa caacgtcctg
     1321 attggggaga acgcgtgggg taaatccagc ttgctggacg ccttaaccct gctgctatcg
     1381 ccagaatcag atctctacca ttttgagcgc gacgatttct ggttcccgcc gggagatatc
     1441 aacgggcgag aacatcatct gcatattatt ttgaccttcc gcgaatcgct gccaggccga
     1501 catcgggttc gccgttatcg gccgctggaa gcgtgctgga cgccatgcac cgatggctat
     1561 caccgtattt tttatcgtct ggaaggggag agtgcggaag acggcagcgt gatgacactg
     1621 cgcagttttc tcgataaaga cggacatccg attgatgtcg aggatattaa cgatcaggca
     1681 cgccatctgg tgcgtttaat gccggtgctg cgcttgcgtg atgcccgttt tatgcgccgt
     1741 attcgtaacg gcacggtgcc aaatgtcccc aatgtggaag tcaccgcgcg ccagctcgat
     1801 ttcctcgccc gtgagttgtc ctcacatccg caaaatctct ctgatggaca gattcgtcag
     1861 ggactttccg caatggtgca gttgcttgag cattatttct ctgagcaggg ggctggacag
     1921 gcgcgatatc gtttaatgcg gcggcgggcc agcaatgagc aacgaagctg gcgctatctg
     1981 gatatcatca accggatgat tgaccgacct ggagggcgct cgtatcgggt tattttgctc
     2041 ggcctgtttg caactttatt gcaggcaaaa ggcacattgc gactggataa agacgcccgt
     2101 ccattgttgc tgattgaaga tccagaaact cgtttacacc ccattatgct ttcagttgcc
     2161 tggcatctgt tgaatcttct gccattgcag cgcattgcca ccaccaactc gggtgagttg
     2221 ctttcgttaa ctccagtgga acatgtttgc cgcctggtgc gtgaatcttc tcgtgttgcc
     2281 gcctggcgtc tggggccaag tggcttgagc accgaagaca gccgccgcat ctcttttcac
     2341 attcgtttta atcgtccgtc atcgctgttt gctcgctgct ggttgctggt ggaaggggaa
     2401 acagaaacct gggttatcaa cgaactggcg cgccagtgtg gacaccattt cgatgccgaa
     2461 gggatcaagg tcattgagtt tgcccagtcc gggctaaagc cactggttaa atttgcccgc
     2521 cgaatgggga ttgaatggca tgtactggtc gatggcgatg aagcagggaa gaaatatgcc
     2581 gctacggtac gcagcctgtt gaataacgat cgggaagccg aacgagaaca tttaacggcg
     2641 ttaccggcgc tggatatgga acattttatg tatcgccagg gattttccga tgtgttccac
     2701 cgcgtggcgc aaatcccgga aaatgtaccg atgaatctgc gcaaaattat ctcgaaagcg
     2761 atccatcgct cttccaaacc cgatcttgcc attgaagtgg caatggaggc cggacgtcgt
     2821 ggtgtggact ccgtaccgac gctgctgaaa aaaatgttct cacgcgtgct gtggctggcg
     2881 cgcggtcgcg cggattaacc gcgaaacatc gtggccattt gtggctgaat agcgtcgagc
     2941 atctcatagc gccgacggta ttcagcccgt tttttactgg cgatttcggc aatctctttt
     3001 cgtgctatct gtgctggaag gcggtaatgg cgttcagcat cacatacgcc gccaaccgat
     3061 tcccagaaag cgttgtaatc ggcatggatc ttgccttctt tatcacgata acgcaggctg
     3121 cggtaaatat gcgtttcatt gctgacggca ataatctgct ctacctgcaa acgttgggca
     3181 aacagacagg ccgcttccat cacgaggcgt ttgggaaata gcccgtggca ggctttcgtc
     3241 gcattctgga tttcctgatg tggaatttcc cattttgcgc cttgcagtcc gccaataaac
     3301 atcgttcttt tcccctgata ttcacacagg gtaaacgtga tctctgccag aggaatacct
     3361 tcgctgttgc gaaacaggat tgtgctgtca ccttctttat ccattgagat catcatggtc
     3421 agctcaagcg tgaactgctc gccgtttttg ccttccagct tcgccagttg cagcccgggg
     3481 gtattcaaat ataagctgaa ttcttccgcc gacatacatc cgcggagtaa cgcataatgg
     3541 taacgtaacg cctccagcaa ttgcttacgg ctaagattcg ccgcaaggta agggcgatgc
     3601 agacgcacag gcagtcgcgg ttggcgcgtt aacaacacat tgagattagg ccagtgggaa
     3661 agttcgttca tccactcaac gcttaaacgc ggcataatca acgagcgcag caaaaatttc
     3721 tggcgaaaac tacggcgatg ccagaattta cccggccgac actgtccacg tgccagacta
     3781 agaaaaagtg acaggctgct gagagattca gatggcgtaa aggtccgttc agttagctgc
     3841 gacatattca tgaaatcaat ggttatacat gacgtcgatt tcaccattgc gtatcttaac
     3901 caaacatcaa tagtgtgatt actaacgtaa attttagggt tttgttgata tttcgttgaa
     3961 gttaatgacc cggattggca tatggagtat tcagaatatt tatgaaaaag cggaaaaccg
     4021 tgaagaagcg ttacgttatt gcgctggtga tagtcatcgc cggactgatt acgttatgga
     4081 gaattcttaa cgcacccgtg ccgacttatc agacactgat tgtgcgcccc ggtgatttac
     4141 agcaaagcgt gctggcgacc ggaaagctgg acgcgctgcg taaggttgac gtgggcgcgc
     4201 aggtcagcgg tcagttgaaa actctgtcgg tggcgattgg cgataaagta aaaaaagacc
     4261 agcttttagg ggttattgat cctgaacagg ctgaaaacca gattaaggag gtcgaagcaa
     4321 cgctgatgga gctacgtgcg cagcggcagc aggcggaagc ggagctgaaa ctggcgcggg
     4381 tgacgtattc ccgtcagcaa cgtctggcac aaacgcaggc ggtttcacag caggatctcg
     4441 acaccgccgc gacggagatg gctgtgaaac aggcgcaaat tgacaccatt gacgcgcaaa
     4501 tcaagcgcaa tcaggcttct ctcgatacgg ctaaaaccaa tctcgattac actcgcatcg
     4561 ttgccccgat ggccggggaa gtcacgcaaa tcaccactct gcaaggccag acggtgattg
     4621 ccgcacaaca agcaccgaac attctgacgc tggcagatat gagcaccatg ctggtaaaag
     4681 cgcaggtttc tgaagcggat gtaatccacc tgaagccggg gcaaaaagcc tggtttacgg
     4741 tgcttggcga tccactgacg cgctacgagg ggcaaatcaa ggatgtacta ccgacgccgg
     4801 aaaaggttaa cgacgctatt ttctattacg cccgttttga agtccccaac cccaatggtt
     4861 tgctgcggct ggatatgact gcgcaagtgc atattcagct caccgatgtg aaaaatgtgc
     4921 tgacgatccc tctgtcggcg ttaggcgatc cggttggcga taatcgttat aaagtcaaat
     4981 tgttgcgtaa tggtgaaaca cgcgagcgtg aagtgacgat tggcgcacgt aacgataccg
     5041 atgttgagat tgtcaaaggg cttgaagcgg gcgatgaagt ggtgattggt gaggccaaac
     5101 caggagctgc acaatgacgc ctttgctcga attaaaggat attcgtcgca gctatcctgc
     5161 cggtgatgag caggttgagg tgctgaaggg catcaccctc gatatttatg ccggtgagat
     5221 ggtagcgatt gttggcgctt caggttccgg taaatcgacc ctgatgaata ttctcggctg
     5281 tctggataag gccaccagcg gcacctatcg cgtcgccggt caggatgttg ccacactgga
     5341 cgccgatgcg ctggcgcaac tgcgccgcga gcatttcggc tttattttcc agcgttacca
     5401 tttactttcg catttaaccg ccgagcagaa cgttgaagta cccgccgtct atgccggtct
     5461 tgagcggaaa cagcggctgc ttcgcgccca ggggttactg caacgactgg ggctggaaga
     5521 ccgtacagag tattatccgg cacagctttc gggtggtcag caacagcgcg tcagcatcgc
     5581 gcgggcattg atgaacggcg gtcaggtaat tcttgccgat gaaccaaccg gcgcactgga
     5641 cagccattct ggcgaagagg tgatggcgat cctgcatcag ctgcgcgatc gcgggcatac
     5701 ggtgattatc gtcacccacg atccgcaggt cgctgctcag gccgagcggg tgatcgaaat
     5761 tcgcgacggc gaaattgtgc gcaatcctcc cgccattgaa aaagtgaatg ttgctggcgg
     5821 gacggagcct gttgtcaaca cggtgtctgg ctggcggcag tttgtcagcg gttttaacga
     5881 ggcgctgacg atggcatggc gggcgctggc agcgaataaa atgcgtactt tactgaccat
     5941 gctggggatt attatcggta ttgcgtcggt ggtttccatt gtcgtggtgg gtgacgccgc
     6001 caaacaaatg gtgctggcgg atattcgttc tattggtacg aatactattg atgtctatcc
     6061 cgggaaagat tttggcgatg acgatccgca atatcagcag gcgctgaagt acgacgactt
     6121 aatcgccatc caaaaacaac cgtgggtcgc ctcagccaca cctgccgtct cgcaaaacct
     6181 gcgcctgcgt tataacaatg ttgatgttgc tgccagtgcc aatggcgtga gcggcgatta
     6241 ttttaatgtc tatggcatga ccttcagtga aggaaacacc tttaatcagg agcagctgaa
     6301 cggtcgtgcg caggtcgtgg ttctcgacag taatactcgc cgccagcttt tcccccataa
     6361 agcagatgtg gttggcgagg tgattctggt cggcaatatg cccgccagag tcattggtgt
     6421 ggcggaagaa aaacagtcga tgtttggtag cagtaaagtg ctgcgtgtct ggctacctta
     6481 cagcacgatg tccgggcgag ttatgggcca gtcgtggctg aactccatta ctgtcagggt
     6541 aaaagaagga tttgacagcg ccgagacgga acagcaactc acgcgtttac tttcactgcg
     6601 ccacggaaag aaggatttct ttacctggaa catggacggc gtcttgaaaa ctgttgaaaa
     6661 gaccacacgt aatttacaac tgtttctgac gctggtggcg gtgatttcgc tggtggtggg
     6721 cggtattggt gtaatgaata ttatgctggt gtcagtgacc gagcggacgc gggaaattgg
     6781 cattcgcatg gctgtaggtg cgcgagcaag ggatgttttg caacagttcc tgatcgaagc
     6841 cgtactggtt tgcctggtcg gtggcgcgtt gggaataact ctgtcactgt taattgcttt
     6901 caccttgcag cttttcttac ccggctggga gattggtttt tcaccgttgg cactgctgct
     6961 ggcgtttctc tgctcgacgg ccaccgggat tttatttgga tggttacccg cacgaaatgc
     7021 ggcacgactg gatccagtag atgctctggc acgagagtaa tttttgagat aaaaatgcca
     7081 gccgatcggg ctggcatttt gcctttagga tgtacacaat gagacagaag agctatgcga
     7141 ctgccgcttc tacttcgacg ggcacaataa cactggcgtg attgcctttt ggcccctggt
     7201 ggacatcaaa ctgaacggat tgtccagctt ttagcgttct gtaaccatcc atctgaatgg
     7261 tggaataatg agcgaaaata tcttcgccgc cgccttcagg gcagatgaaa ccaaaccctt
     7321 tggcattgtt gaaccactta acagtaccct tttccatgct tcgacatcct tcgcaaatct
     7381 tatacaagta agatggaata aaccggggtc agagaggggg ctgttcaaaa cctcgccaac
     7441 tctagaaata caatttagag aattagggcg agccgtcaag cgtttgacag gggccagggg
     7501 gcaggtatga atcaaaaatt tgaagcagtt aacgctattg acaggaatgt gacagatgtc
     7561 gctgatgcca acgatagatg atagttatct atcatgtgga gtagattggt caggcaaata
     7621 agctcttgtc agcggcaggg cgttctgccg ataaccgtaa ccgaagatga taactgacaa
     7681 tgggtaaaac gaacgactgg ctggactttg atcaactggc ggaagaaaaa gttcgcgacg
     7741 cgctaaaacc gccatctatg tataaagtga tattagtcaa tgatgattac actccgatgg
     7801 agtttgttat tgacgtgtta caaaaattct tttcttatga tgtagaacgt gcaacgcaat
     7861 tgatgctcgc tgttcactac caggggaagg ccatttgcgg agtctttacc gccgaggttg
     7921 cagaaaccaa agtggcgatg gtgaacaagt acgcgaggga gaatgagcat ccattgctgt
     7981 gtacgctaga aaaagcctga atgcaggcat aaaaattggg ggaggtgcct atgctcaatc
     8041 aagaactgga actcagttta aatatggctt tcgccagagc gcgcgagcac cgtcatgagt
     8101 ttatgaccgt cgagcacttg ttactggcgc tgctcagtaa cccatctgcc cgggaggcgc
     8161 tggaagcgtg ttctgtggat ttggttgcgc tccgtcagga actggaagcc tttattgaac
     8221 aaaccacacc cgttctgcct gccagtgaag aggagcgcga cacacagccg acgctgagtt
     8281 ttcagcgtgt actgcaacgt gcggtcttcc atgtccaatc ctccggtcgc aatgaggtaa
     8341 ccggtgcaaa cgttctggtc gctatcttta gcgaacagga gtcgcaggcg gcatatctgt
     8401 tgcgtaaaca tgaagtcagc cgtctcgatg tggtgaactt tatctctcat ggcacgcgta
     8461 aagacgagcc gacacagtct tctgatcctg gcagccagcc aaacagcgaa gaacaagctg
     8521 gtggggagga acgtatggag aatttcacga cgaacctgaa tcagcttgcg cgcgtgggcg
     8581 gaatcgaccc actgattggt cgtgagaagg agctggagcg tgctattcag gttctctgcc
     8641 gtcgccgtaa aaacaacccg ctgctggtgg gggaatctgg tgtcggtaaa accgcgattg
     8701 cggaaggtct tgcctggcga attgttcagg gcgatgtgcc ggaagtgatg gctgactgta
     8761 cgatttactc tctcgatatc ggttctctgt tagcgggcac taaatatcgc ggcgactttg
     8821 aaaaacgttt taaagcgttg ctcaagcagc tggagcagga cactaacagc atcctgttta
     8881 ttgatgagat ccacaccatt atcggtgcgg gggcagcgtc tggtggccag gtcgatgcgg
     8941 ctaacctgat caaaccgttg ctctccagcg gtaaaattcg cgtaattggt tcgacaacct
     9001 atcaggagtt cagcaacatt ttcgagaaag accgtgctct ggcgcgtcgc ttccagaaaa
     9061 ttgatattac tgaaccgtcg atcgaagaaa ctgttcaaat catcaatggc ctgaaaccga
     9121 agtatgaagc gcaccacgac gtgcgttata ccgcaaaagc ggtgcgtgca gcggtagagc
     9181 tggcggtgaa atacattaac gatcgtcatc tgccggataa agccattgac gttatcgacg
     9241 aagcgggcgc tcgcgcacgc ctgatgccgg taagcaaacg caagaaaacc gttaatgtgg
     9301 cggatattga gtccgtggtg gcccgtattg cgcgcattcc agagaagagt gtttctcaga
     9361 gtgaccgcga taccctgaaa aacctcggcg atcgcctgaa aatgctggtc ttcggtcagg
     9421 ataaagccat tgaggcgctg actgaagcca ttaagatggc gcgtgcaggt ttaggtcacg
     9481 aacataaacc ggttggttcg ttcctgtttg ccggccctac cggggtcggg aaaacagagg
     9541 tgacggtaca gctttcgaaa gcgttgggca ttgagctgct gcgctttgat atgtccgagt
     9601 atatggaacg ccataccgtc agccgtctga ttggtgcgcc tccgggatac gttggttttg
     9661 atcagggagg tttgctgact gatgcggtca tcaagcatcc acatgcggtg ctgttgctgg
     9721 acgaaatcga gaaagcgcat ccggacgtgt tcaatattct gttgcaggtg atggacaacg
     9781 gtacgctgac cgataacaac ggacgcaaag cggacttccg taacgtggtg ctggtgatga
     9841 ccaccaacgc cggggtacgt gaaactgagc gtaaatccat tggtcttatc caccaggata
     9901 acagcaccga tgcgatggag gagatcaaga agatctttac accggaattc cgtaaccgtc
     9961 tcgacaacat tatctggttt gatcatctgt caaccgacgt gatccatcag gtggtggata
    10021 aattcatcgt cgagttgcag gttcagctgg atcagaaagg tgtttctctg gaagtgagcc
    10081 aggaagcgcg taactggctg gccgagaaag gttacgaccg ggcaatgggc gcacgtccga
    10141 tggcgcgtgt catccaggac aacctgaaaa aaccgctcgc caacgaactg ctgtttggtt
    10201 cgctggtgga cggcggtcag gtcaccgtcg cgctggataa agagaaaaat gagctgactt
    10261 acggattcca gagtgcacaa aagcacaagg cggaagcagc gcattaatct gattgttagg
    10321 taggttggtc aagtccgtaa tctcgaaaga ggttacggac tttttgttta tggggtggag
    10381 gaggttcaga cccttttttt aatgatgatg gtaagttgtt gataattagt gctgcgggaa
    10441 ggtaaggata aaaaagggtg ctgcaggaga atgggatggt tttgctttat taacaacggg
    10501 ctaaacgtgt agtatttgag ttcactgccg tacaggcagc ttagaaatcg acggggtgcg
    10561 gtaaaacctt tgcgaacgcg ttcactgccg tacagacaga taaaatgcga aaaaaaagct
    10621 cgcactttcg tacgagctct tctttaaata tggcggtgag ggggggattc gaacccccga
    10681 tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa
    10741 attgttttgt cgcctgacct catgggtggc aacggggcgc tactataggg agttggagta
    10801 aaacggtcaa gaagaatttt aatgataatt attgtttgct catactgtaa acaagttgtg
    10861 cagtatatct acatcgagac aagttacgga cttatacttc caaagtactt catacatatc
    10921 acaaaataaa aaggccggtt aaaccgacct tttactcgtt ctttctcttc gcccatcagg
    10981 cggtaaaaca atcagcgact acggaagaca atgcggcctt tgctcaggtc gtacggggtc
    11041 agttcaacag tcactttgtc gcccgtcagg atgcggatgt agtttttgcg cattttaccg
    11101 gagatgtgtg cagtaaccac gtgaccgttt tctaactcta cgcggaacat ggtattaggc
    11161 aacgtttcaa gaacggtacc ttgcatttca atattgtctt ctttggccat ctaatcctct
    11221 ggggtatcac taccgtaatt tgaaccggca agataatgcc gaagttctgt aaataagtaa
    11281 agatttgcgc gctaaatcgc aacaaacagg ttcggcacat tactccgaaa acacacggct
    11341 aagccgcacc aaaagcgcaa cgtataaggg agcggtgaga taaacgatgg gcgttacctg
    11401 acgcgaaaaa ttccttatcg gcagcggggt aatgagcgta accaactctg cgaccgcaat
    11461 tataacactc tggggagaaa tgtgccgaaa acattcattc ttgtggtgaa aacaagcatc
    11521 gtggtaccca gaaattattc ggcaatcgtc cgaggctcat ttgattgaga taattaaggt
    11581 aatcccggcg gggaatttcg caggcaccaa gcgatgctgt gtgatcgtta aggacctggc
    11641 agtcgataag cttaccgcca tgaccgataa attcctcaca gaataccaga agcgccgttt
    11701 tagacgcatt ttccatccgg ctgaacatgg actcgccaca aaatagcgtt ccctgggcca
    11761 cgccgtacat accgccgaca agctcatctt cacgccagac ttcaatggag tgggcatggc
    11821 cgagttcgtg aaggcgatgg taggcttcga ccacgccacg cgtgatccag gttccttctt
    11881 cgcgatcgct ggcacagcct tcaatgacct gaccaaaagc gtaattcatc gtgacacgat
    11941 agggcgagcg tttatgaaat cgcttcatac tacggctgat atgcagtgat tctggccata
    12001 gcaccgcgcg gggatcgggc gaccaccaga ggatggggtc gcctggagaa aaccacggaa
    12061 aaataccacg ctggtaagcc attaacaggc gcgcagggct aagatcgccc ccaagtgcca
    12121 gcaggccgtt aggctcacgt aatgcgcctt ccggggaagg gaaggctatt gaatggcgag
    12181 aaagctgaac caggcgcatg accgcaaaac tccacgcaag tcggatcgtt caataatagc
    12241 ttacaaaccc tgcttgaact ggtaataacg cccctgtctg gcaagcagct ctgcgtgagt
    12301 accttgctca ataatttgcc cgttgtccat cactattatt tgttggaaac gagagagtcc
    12361 gcgaagtcga tgggtgacca ttaacaccgt tttctcacgc atcatttctg caagcaattc
    12421 aaggatctgg ctttcggttg tggcatctaa gccttcggta ggttcatcca gcaacaccag
    12481 tggcgcatca tgtaacagcg cacgggcgat agccagacgg cgcagttcac caccggagag
    12541 ctggcgtccg ccttcaccta accaactgtt gagacctgca tcctcgagca gcttctccag
    12601 gccaacgcga cgcaagctct ccgccagagc ctcatcacta ctgccaggcg aggcgagtaa
    12661 cagattatca cgcagcgtgg cgctaaacag atgcactcgc tgaggaacaa cgctgatggt
    12721 ctgtcgtaga gccgcttcat tcaggctggc tatggggcta tcgttaagca aaatctcgcc
    12781 ctgttgcggg tcccaagcgc gggtcagcag ttgtaacagt gttgatttgc cgcatccggt
    12841 tcgcccgaga atcgcaatat gttccccggc gtttacctga agagaaatcc ctttaagtgc
    12901 ctgttgggat tgctccggat aagtgaactg aacatcccgt aatgtcagcg aaacgcgatc
    12961 ggcaacacga gtttgggtat cagggaaggt gacttccggt ttttgatccg ttaaatcagt
    13021 gatccgaacg gcagaggcaa tgacttgccc cagatgctga aatgcacccg ttactggtgc
    13081 cagtgcttca aacgcggcta acgcgcagaa gacaaacagg gcaattaacg cgccgggttg
    13141 agcattgccg ccaacgccgc cagacgccat ccacagcatc aggatcaccg ctaacgcgcc
    13201 aatgagtagc attatcgctt gcgacaatgc ggtcagttca gattgacggc gttgcgcttc
    13261 cagccattga atttctgtat tctctagttg cgtgcgataa cgatcgctgg caccaaaaat
    13321 ggtcagctca gcttgccctt gcagccaggc cgtcagttgt tggcgatact gtccgcgaag
    13381 atgagtcaga ttttgcccgg tgctttttcc cgcacgataa aacagcggtg gcatcaggaa
    13441 aagcgtcagt aacataatgc cgcccagcgt aaaggcgagg gtgaaatcaa ggaaacttaa
    13501 cccgattgtc accaccataa tcaccacaaa agcgcccacc agcggcgaga taacgcgcag
    13561 ataaagatga tcgagcgtat caacatccgc caccacgcga ttgagcaatt cgccctgacg
    13621 atagcgcgcc agtccggcag gggagagggg cagcaatttg ctgaaggtgt aaatgcgcag
    13681 atgctgcaac acgcggaaag tcgcgtcgtg acttaccaga cgttcaaaat aacgcccggc
    13741 agtacgggtg attgctgcgc cacgcacgcc cgcagcgggt agcatatagt tgaagctgta
    13801 cagtccggca acccccgcaa ccgctgaggc cgagaggaac cagccggaaa gtgtcaacag
    13861 accgatactg gcgagcagcg tgacaattgc cagcacgata ccaagactta acatccattt
    13921 atgacgttta tacagtgcca gatagggtag caaagcgcgc atttaaatct cctcctgacg
    13981 atgggccagt aatgtggcga atgggccacc agccacactt aattccgcgt aacgtccttg
    14041 ctcaataatc cggccatcct gcataaccca aatgacatcc cagtcagcaa gatcttctaa
    14101 ctggtgggtg accattaacg ttgtctggcg cagagaggcg gcattcagcg cctccattac
    14161 gcgctgttca ctgtgagcat caaggctggc agcgggttca tccaacagta atagcgaaca
    14221 gggatttagt aacgcacggg ccaccgccac gcgctgcgcc tgccccacgg aaaggcgggc
    14281 agcctggtcg ccaacaggcg tatcaacgcc ttgtggcagg agcggtagaa actcgctgac
    14341 ccaggcgtta tccagcgctg cttgtaattc ttgttcgctg gcatcaggtc gcgccagtag
    14401 tacgttatcc cgcaatgttg ctgccggtaa ttgtgggttt tgcccaaccc aggagagatg
    14461 tttacgccat gattctggtg ataaatcgcg taattctatc ccgttgattc gtaatgatcc
    14521 ctgatatgaa agaaaaccag aaagcgcgtt cagcagtgag cttttacctg aaccgctgcg
    14581 accaaccaac actgcacgtt ggcctgctgg caaagtaaag ttcagcggtc cggccagcgt
    14641 tttaccttcc ggcgacgtga taaacagatc ctcggcctca atggtcaccg gatcggtcga
    14701 tgctaattcc gcctcaccgc gctgcggatg ggcgagcggg gtttccataa acgttttcag
    14761 actgtcagct gcaccaacag cctgggcttt agcatgataa aacgtaccga gatcgcgcaa
    14821 tggctggaaa aactctggcg caaggatcag ggccagaaaa cccgcagcca gcgtcacacc
    14881 ggtatcgtag tgaccaaaat ccagctcgcc gagataggaa aaaccaaagt agaccgccac
    14941 cagagcaatt gacagcgagg taaaaaattc gagaatgccg gaggataaaa acgccagccg
    15001 tagcacttcc attgtccgtt ggcggaaatc ttccgaagca gaacgaatac tttcaatttc
    15061 agcttcacca cgaccaaaaa tacgcaatgt ttccatgccg cgcaggcgat cgaggaaatg
    15121 cccacttaag cgagcaagag cgagaaagtt acgccggtta gcatcggcag cccccatccc
    15181 aaccagcgcc ataaacaacg gaattaacgg tgctgtgccc agcagaatga gcgccgcagc
    15241 ccagttagag gggaagatcg ccaccacaat cagcaacggc accgacactg ccagcgccat
    15301 ttgcggcagg tagcgtgcat agtaatcatg catatcgtca atttgctcga gtaccaacgt
    15361 cgcccagctc cccgcaggtt taccctgaat ccacgctggc cctgcttgtt gcagacggtc
    15421 gagaacctga cggcggatgg caaagcggat atgctgcccg gcgtgataac ccacccgttc
    15481 gcgtaaccag accacccatg cgcgcagtac aaaggtcaga accagtaacg taaagggaag
    15541 cagcagggct tcacggggaa tattctccat aatcatatgt tgcagaattc gcgccatgaa
    15601 ccaggcctgg gcaatgatca atatgccgct tacaaagccc agcagacgag aaatattcag
    15661 ccaacgttgg gagatgacgc tttgctgttt taaccagcgg gttaactctt tttgacggga
    15721 tttattcatt gcacgcttag caggtgagtt atcagaatta tttgcagagc aatgttacaa
    15781 cggggaaaaa ataaaggcga cccatagtcg catggtgtcg ccttctttac ttttgttact
    15841 gatttgtaaa attattttgc gtcagctaaa ccatcgaggt agcgttccgc atcaagtgct
    15901 gccatgcagc ctgtaccggc cgaagtaatg gcctgacgat aaatatgatc catcacgtca
    15961 cccgcggcaa agacgccagg gatgctggtc tgggtggcat taccatgaat acccgactgc
    16021 actttgatgt agccgttttc cagttccagc tgcccttcga aaatggcagt attcgggctg
    16081 tgaccgatag caacaaacag accagcaacg tcgagtgact cgatgttatc gctgttttgc
    16141 gtatcgcgca gacgaacgcc agtgacaccc atttgatcgc cggtcacttc ttccagcgta
    16201 cggttggtgt gcagaatgat gttgccgttc tccactttat ccatcagacg cttaataagg
    16261 attttttccg cgcggaaacc gtcacggcgg tgaatcagat gcacttccga agcgatgtta
    16321 gacagataca gcgcctcttc aaccgcggta ttgccgccgc cgatgaccgc aactttctgg
    16381 ttgcgataga agaaaccgtc gcaggttgca caagcagaaa ccccacggcc tttaaaggct
    16441 tcttcagagg gcaggccgag atagcgtgca gaagctccgg tggcaataat cagcgcgtcg
    16501 caagtgtatt cgccgttatc gccattcaga cggaacggac ggttttgcag atccaccttg
    16561 ttgatatgat caaaaatgat ctcagtttca aacttggtgg catgttcgtg catgcgctcc
    16621 attaataacg gaccggtcag atcgtttgga tcgccaggcc agttttccac ttccgtggtg
    16681 gtggtcagtt ggccgccttt ttccatgccg gtaatcagca caggttgcag gttggcgcgc
    16741 gccgcgtaga cagcagcggt gtatcccgcc gggcctgaac ccaggataag cagtttactg
    16801 tgtttggtcg tgcccatgag atccccatag ttgttggcag acaatgggca ggattgtagg
    16861 gaatttacag acgtaaaaaa agagtatgac gattttgtta acaatttgtg caatcggtag
    16921 catcgataag caggtcaaat tctcccgtca ttatcacctc tgctacttaa atttcccgct
    16981 ttataagccg attaaatgat gaataaacgc ccctgttaat gaatatctgg catgttgtac
    17041 taaaaatcga tgttttgctt tgacaatccc ctggtgtttt gcgaaaacat tcgaggaaga
    17101 aaaaaaacag tattcttata tgcgcataac catgcatgta aataccatgt ttaccgtgct
    17161 agtgaaatct acgtatggcg tggacagacg ccattcgtga tgtcgatagc tgccacaagg
    17221 caacggtctt ctcaccgtag acccaggcat tgcgcgccgt gaatcttcat gatttcggtc
    17281 tatcgtgacg ggtagcgact ctgaacagtg atgtttcagg gtcagacagg agtagggaag
    17341 gaatacagag agacaataat aatggtagat agcaagaagc gccctggcaa agatctcgac
    17401 cgtatcgatc gtaacattct taatgagttg caaaaggatg ggcgtatttc taacgtcgag
    17461 ctttctaaac gtgtgggact ttccccaacg ccgtgccttg agcgtgtgcg tcggctggaa
    17521 agacaagggt ttattcaggg ctatacggcg ctgcttaacc cccattatct ggatgcatca
    17581 cttctggtat tcgttgagat tactctgaat cgtggcgcac cggatgtgtt tgaacaattc
    17641 aataccgctg tacaaaaact tgaagaaatt caggagtgtc atttagtatc tggtgatttc
    17701 gactacctgt tgaaaacacg cgtgccggat atgtcagctt accgtaagtt gctgggggaa
    17761 accctgctgc gtctgcctgg cgtcaatgac acacggacat acgttgttat ggaagaagtc
    17821 aagcagagta atcgtctggt tattaagacg cgctaacacg gaacaggtgc aaaatcggcg
    17881 tattttgatt acactcctgt taatccatac agcaacagta ctggggtaac ctggtactgt
    17941 tgtccgtttt agcatcgggc aggaaaagcc tgtaacctgg agagcctttc ttgagccagg
    18001 aatacactga agacaaagaa gtcacattga caaagttaag tagcggccgt cgccttctgg
    18061 aagcgttgct gatccttatt gtcctgtttg ccgtctggtt gatggctgcc ttactaagct
    18121 ttaacccttc ggaccccagc tggtcgcaaa cggcctggca tgaacctatc cataatttag
    18181 gtgggatgcc cggtgcgtgg ctggcagata cgctgttctt tatttttggc gtgatggctt
    18241 acaccattcc cgtcattatt gtcggcggtt gttggtttgc ctggcgtcat cagtccagtg
    18301 acgaatacat tgattatttt gccgtttcgc tacgcattat tggcgttttg gcgctcatcc
    18361 ttacctcctg tggtctggcg gcaatcaacg ctgacgatat ctggtatttt gcctccggtg
    18421 gcgtcattgg cagcttacta agcactacgc tacaaccact gctacacagt agcgggggaa
    18481 ctattgcgct gctctgcgtt tgggctgcgg gcctgacgct gttcaccggt tggtcatggg
    18541 tgaccattgc tgaaaaactc ggcggctgga ttttaaacat tctcaccttc gccagtaatc
    18601 gtacccgtcg cgatgatacc tgggtcgatg aagatgaata tgaagatgac gaagagtatg
    18661 aagatgaaaa ccacggcaaa cagcatgaat cacgccgtgc ccgtattctt cgcggcgcgc
    18721 tagcgcgtcg taaacggttg gcggaaaaat tcattaatcc gatggggcgg caaacagacg
    18781 ctgcgttgtt ctccggtaag cggatggatg atgacgaaga gattatctac actgcacgcg
    18841 gtgtggctgc cgacccggac gacgtcctat tttcgggcaa tcgtgcaacg cagccagaat
    18901 atgacgaata cgatccatta ttaaacggtg cgccaattac cgaacctgtc gctgtagcag
    18961 ctgctgctac cacggcgaca caaagctggg ctgcgccggt tgaacctgtg actcagacgc
    19021 cgcctgttgc ctctgttgat gttccacctt cgcaacctac agtagcctgg cagcctgtac
    19081 cgggtccaca aacgggagag ccggttattg ctcctgcacc ggaaggttac ccacagcagt
    19141 cacaatatgc gcagcctgca gtgcaatata atgagccgct gcaacaacca gtacagccgc
    19201 agcagccgta ttatgcacct gcagctgaac aacctgcgca acaaccttac tacgctcctg
    19261 cggcggaaca acctgttcag caaccgtatt acgcccctgc gccagaacaa ccggtggcag
    19321 gtaacgcctg gcaagccgaa gagcagcaat ccacttttgc tccacagtct acataccaga
    19381 ctgagcaaac ttatcagcaa ccagccgctc aggagccgtt gtaccaacag ccgcaatccg
    19441 ttgaacagca gcctgttgtg gagcctgaac ccgttgtaga agagacaaaa cccgcgcgtc
    19501 cgccgcttta ctattttgaa gaagtggaag agaagcgagc ccgtgaacgt gaacaacttg
    19561 cggcctggta tcaaccgatt ccagaaccgg ttaaagaacc agaaccgatc aaatcttcgt
    19621 tgaaagcgcc ttctgttgca gcagtacctc cagtagaagc cgctgccgct gtttctccgc
    19681 tggcatctgg cgtgaaaaaa gcgacactgg cgacgggggc tgccgcaacc gttgccgcgc
    19741 cagtcttcag tctggcaaat agcggtggac cgcgtccgca agtcaaagag gggattggcc
    19801 cgcagttgcc acgaccgaaa cgtatccgcg tgccaactcg tcgtgaactg gcgtcttacg
    19861 gtattaagct gccctcacag cgtgcggcgg aagagaaagc ccgtgaagcc cagcgcaatc
    19921 agtatgattc tggcgatcag tataacgatg atgaaatcga tgcgatgcag caggatgaac
    19981 tggcacgtca gttcgcccag acacagcagc aacgctatgg cgaacagtat caacatgatg
    20041 tgcccgtaaa cgcagaagat gcagatgctg cggcagaggc tgaactggct cgtcagtttg
    20101 cgcaaactca acaacaacgt tattccggcg aacaaccggc tggggcgaat ccgttctcgc
    20161 tggatgattt tgaattttcg ccaatgaaag cgttgctgga tgatggtcca cacgaaccgt
    20221 tgtttacgcc aattgttgaa cctgtacagc agccgcaaca accggttgca ccgcagcagc
    20281 agtatcagca gccgcaacaa ccagttccgc cacagcagca atatcagcag ccgcaacaac
    20341 cggttgcacc gcagcagcag tatcagcagc cacaacagca ggttgcgccg cagccacaat
    20401 atcagcagcc gcaacaaccg gttgcgccac agccgcaata tcagcagccg caacaaccag
    20461 ttgcgccgca gcagcagtat cagcagccgc aacaaccggt tgcaccgcgg cagcaggata
    20521 ccctgcttca tccgctgttg atgcgtaatg gcgacagccg tccgttgcat aaaccgacga
    20581 cgccgctgcc ttctctggat ttgctgacac cgccgccgag cgaagtggag ccggtagata
    20641 cctttgcgct tgaacaaatg gctcgtctgg tggaagcgcg tctggctgat ttccgtatta
    20701 aagccgatgt cgtcaattac tctccggggc cggttatcac tcgctttgaa cttaacctgg
    20761 ctcctggcgt aaaagcggcg cgcatttcta acttgtcacg ggaccttgcc cgttcacttt
    20821 cgacggtggc ggtgcgtgtc gttgaagtta ttcctggcaa accctatgta ggtctggagt
    20881 taccaaataa aaaacgacaa accgtttatc tgcgcgaagt tttggataac gccaaattcc
    20941 gcgataatcc gtcgccatta accgtggtgc tgggtaaaga tatcgccggt gagccggtgg
    21001 ttgccgatct ggcgaaaatg ccgcacttgt tggttgcggg gactaccggt tccggtaaat
    21061 ctgtcggtgt gaacgcgatg atcctgagca tgctttataa agcacagcca gaagatgtgc
    21121 gtttcatcat gatcgacccg aaaatgctgg agctttcggt gtatgaaggc attccgcacc
    21181 tgttaacgga agtcgttact gatatgaaag atgccgccaa cgcgctgcgc tggtgtgtta
    21241 acgagatgga gcgtcggtat aaactgatgt ctgcgctggg tgtgcgtaat ctggcgggtt
    21301 ataacgaaaa aattgctgaa gccgatcgca tgatgcgtcc gattccagac ccgtactgga
    21361 agccgggtga cagtatggat gcccagcatc cggtgctgaa aaaagaacca tacattgtgg
    21421 tgttggttga cgaatttgcc gacctgatga tgacggtagg taaaaaagtg gaagagctga
    21481 tagcacgtct ggcgcaaaaa gcccgtgccg cgggtatcca cctcgtactg gcaactcagc
    21541 gtccatcggt tgatgttatt actggtctga ttaaagcgaa tattccgacc cgtatcgcct
    21601 ttaccgtatc cagtaagatt gactcacgta ccattcttga tcaggctggc gcggaatcac
    21661 tgctgggtat gggggatatg ctctactctg ggccgaactc cacgttgccg gtacgtgtcc
    21721 atggtgcttt tgttcgcgat caggaagttc atgccgtggt gcaggactgg aaagcgcgtg
    21781 gtcgcccaca gtatgttgat ggcatcacct ccgacagcga aagcgaaggt ggtgcgggtg
    21841 gtttcgatgg cgctgaagaa ctggatccgt tgttcgatca ggcggtgcag tttgtcactg
    21901 aaaaacgcaa agcgtcaatt tctggcgtac agcgtcagtt ccgcattggt tataaccgtg
    21961 cagcacgtat tatcgaacag atggaagcgc aggggattgt cagcgaacag gggcacaacg
    22021 gtaatcgtga agtgctggcc ccaccgccgt ttgactaatt aatgcattgc cggataaggc
    22081 gcggtagcgt cgcatccggc actctatcaa ctgaaaattc agtattttct tctttcctca
    22141 agctgattat tagcctggaa tagagagtag agggaactcc cgatcgggag tgacgtaatt
    22201 tgaggaataa tgatgaaaaa aattgccatc acctgtgcat tactctcaag cttagtagca
    22261 agcagcgttt gggctgatgc cgcaagcgat ctgaaaagcc gcctggataa agtcagcagc
    22321 ttccacgcca gcttcacaca aaaagtgact gacggtagcg gcgcggcggt gcaggaaggt
    22381 cagggcgatc tgtgggtgaa acgtccaaac ttattcaact ggcatatgac acaacctgat
    22441 gaaagcattc tggtttctga cggtaaaaca ctgtggttct ataacccgtt cgttgagcaa
    22501 gctacggcaa cctggctgaa agatgccacc ggtaatacgc cgtttatgct gattgcccgc
    22561 aaccagtcca gcgactggca gcagtacaat atcaaacaga atggcgatga ctttgtcctg
    22621 acgccgaaag ccagcaatgg caatctgaag cagttcacca ttaacgtggg acgtgatggc
    22681 acaatccatc agtttaccgc ggtggagcag gacgatcagc gcagcagtta tcaactgaaa
    22741 tcccagcaaa atggggctgt ggatgcagcg aaatttacct tcaccccgcc gcaaggcgtc
    22801 acggtagatg atcaacgtaa gtagaggcac ctgagtgagc aatctgtcgc tcgatttttc
    22861 ggataatact tttcaacctc tggccgcgcg tatgcggcca gaaaatttag cacagtatat
    22921 cggccagcaa catttgctgg ctgcggggaa gccgttgccg cgcgctatcg aagccgggca
    22981 tttacattct atgatcctct gggggccgcc gggtaccggc aaaacaaccc tcgctgaagt
    23041 gattgcccgc tatgcgaacg ctgatgtgga acgtatttct gccgtcacct ctggcgtgaa
    23101 agagattcgc gaggcgatcg agcgcgcccg gcaaaaccgc aatgcaggtc gccgcactat
    23161 tctttttgtt gacgaagtgc accgattcaa caaaagccag caggatgcat ttctgccaca
    23221 tattgaagac ggcaccatca cttttattgg cgcaaccact gaaaacccgt cgtttgagct
    23281 taattcggca ctgctttccc gtgcccgtgt ttatctgttg aaatccctga gtacagagga
    23341 tattgagcaa gtactaactc aggcgatgga agacaaaacc cgtggctatg gtggtcagga
    23401 tattgttctg ccagatgaaa cacgacgtgc cattgcagaa ctggtgaatg gcgacgcgcg
    23461 gcgggcgtta aatacgctgg aaatgatggc ggatatggcc gaagtcgatg atcgcggtaa
    23521 gcgggtcctg aagcctgaat tactgaccga aatcgccggt gaacgtagcg cccgctttga
    23581 taacaaaggc gatcgctttt acgatctcat ttccgcactg cataagtcgg tacgtggtag
    23641 cgcacccgat gcggcgctgt actggtatgc gcgaattatt accgctggtg gcgatccgtt
    23701 atatgtcgcg cgtcgctgtt tggcgatagc gtctgaagat gtgggtaacg ctgatccacg
    23761 agcgatgcag gtggcaattg cggcctggga ttgctttact cgcgttggcc cggcggaagg
    23821 tgaacgcgcc attgctcagg cgattgttta cctggcctgc gcgccaaaaa gcaatgctgt
    23881 ctacactgcg tttaaagccg cgctggccga tgctcgcgaa cgcccggatt atgacgtacc
    23941 ggttcatttg cgtaatgcgc cgacgaaatt aatgaaggaa atgggctatg ggcaggaata
    24001 tcgttacgct catgatgaag caaacgctta tgctgccggt gaggtttact tcccgccgga
    24061 aatagcacaa acacgctatt atttcccgac aaacaggggc cttgaaggca agattggcga
    24121 aaagctcgcc tggctggctg aacaggatca aaatagcccc ataaaacgct accgttaatg
    24181 ttatcgttgc ggtaatgttg ttactgtatc cctgtggtcg caggctgtgg ccacatctcc
    24241 catttaattc gataagcaca ggataagcat gctcgatccc aatctgctgc gtaatgagcc
    24301 agacgcagtc gctgaaaaac tggcacgccg gggctttaag ctggatgtag ataagctggg
    24361 cgctcttgaa gagcgtcgta aagtattgca ggtcaaaacg gaaaacctgc aagcggagcg
    24421 taactcccga tcgaaatcca ttggccaggc gaaagcgcgc ggggaagata tcgagccttt
    24481 acgtctggaa gtgaacaaac tgggcgaaga gctggatgca gcaaaagccg agctggatgc
    24541 tttacaggct gaaattcgcg atatcgcgct aaccatccct aacttgcctg cagatgaagt
    24601 gccggtaggt aaagacgaaa atgacaacgt tgaagtcagc cgctggggca ccccgcgtga
    24661 gtttgacttt gaagttcgtg accatgtgac gctgggtgaa atgcactctg gcctcgactt
    24721 tgcagctgca gttaagctga ctggttcccg ctttgtggta atgaaagggc agattgctcg
    24781 catgcaccgc gcactgtcgc agtttatgct ggatctgcat accgaacagc atggctacag
    24841 tgagaactat gttccgtacc tggttaacca ggacacgctg tacggtacgg gtcagctgcc
    24901 gaaatttgct ggcgatctgt ttcatactcg tccgctggaa gaagaagcag acaccagtaa
    24961 ctatgcgctg atcccaacgg cagaagttcc gctgactaac ctggtgcgcg gtgaaatcat
    25021 cgatgaagat gatctgccaa ttaagatgac cgcccacacc ccatgcttcc gttctgaagc
    25081 cggttcatat ggtcgtgaca cccgtggtct gatccgtatg caccagttcg acaaagttga
    25141 aatggtgcag atcgtgcgcc cggaagactc aatggcggcg ctggaagaga tgactggtca
    25201 tgcagaaaaa gtcctgcagt tgttgggcct gccgtaccgt aaaatcatcc tttgcactgg
    25261 cgacatgggc tttggcgctt gcaaaactta cgacctggaa gtatggatcc cggcacagaa
    25321 cacctaccgt gagatctctt cctgctccaa cgtttgggat ttccaggcgc gtcgtatgca
    25381 ggcacgctgc cgcagcaagt cggacaagaa aacccgtctg gttcataccc tgaacggttc
    25441 tggactggct gttggtcgta cgctggttgc agtaatggaa aactatcagc aggctgatgg
    25501 tcgtattgaa gtaccagaag ttctgcgtcc gtatatgaac ggactggaat atattggcta
    25561 atacccaatt tttctgaatc taaaaagcgc ctgcgggcgg tttttgcatg agattaatta
    25621 actgaaaata aaagggattt attttcaggt gtccatataa tgtccatctt tgaaacaaaa
    25681 aagcccgcag tttttacgct acgggctttg tttttctttg tgacttgcgt gtgagggtgc
    25741 tactttcgac cgaacctttg gcaaccggat agcgggggct ttcacccccg ctgcggtttc
    25801 ttacggttta cactgcaaga acgccgcaaa ttctgaccac atgatgttaa gcctgaactc
    25861 acacggagaa cggtgtatca tccagacggt caacaacatg gttaagcata aaatgctcac
    25921 gatgatcgtt ttttgcggca tagcgcttga ctccttttac agggaggcgc taaccttcca
    25981 cttgcttagg gtagatggtc agggcctcgg gttaacatta aagttgactc ggggcctttc
    26041 cacatccggc cttcaggtaa tccttccagc catcagccga aaggcacccg cgtgtaatgt
    26101 actgatttcc ctttgtgtgc gcaatgcctg gcgttgaagc agacaacgct atcgcactgg
    26161 tgatattctt tcgcacaggc ttactctgtc ctgtgtcggg gcgatgaaaa tgattctttc
    26221 aagaaaaaaa attgaaattg aggttgtgca aagtggaatg gctgataatc ctgagactat
    26281 tacaaaaata aaggtttcac gcccggaaga tcttgaagcg tgtggtgtta tcgtggaaaa
    26341 aatttgtgct attcatgcaa aatcagttaa taccactgcc agtaacgacc acaataaata
    26401 caactgaaca aaaaggcccg catgtcgcgg gcaaatacgt ctgtaatcca acggagaaag
    26461 ggtgccatat cgaccgaacc cggcaaccgg atagcggggg ctttcacccc cgctgcggtt
    26521 tcttactgtt tacactgcaa gaacgccgca aattgtgacc acatgatgtt aagcctgaac
    26581 tcacacggtg acgtgtgtat catccagaca atcatcaaaa tagttatgca taaaatgctc
    26641 actatgatcg ttttttgcgg catagcgctt gactcctttt acagggaggc gctaaccttc
    26701 cacttgcgag gtagatgggt tagggcctcg ggttaaacaa atgtttgact cggggccttt
    26761 ccacatccgg ccttcaggta atccttccag ccgtcagccg aaaggcaccc gcgcgtaatt
    26821 taccttttac gtccgttacg tgcaacggat gacgtggctg tttttccttc cccttcgcca
    26881 tcctttttcg gagcagttgt gcaaaagtgt gcaaaagtga tttttgctgc cactccccgc
    26941 caggccgcgc cagttctggc tttcctgatt ttttcgtttt cgtcgccgga tcctttttca
    27001 gatcttcgat cgtgacgtaa ttttaaaaat ctcttgttat tcagtgcatt aaaatttttc
    27061 gggatcctca gaaagccgga gtttttgcac acttttgcaa aaccttgcac aaagtgcaaa
    27121 accattcaga agggaaaacc tcagtaatgg cgcgccctgg cggggtgttt tgcacccatt
    27181 tcgatttgca aaatttatcg tacacaaata ccggaggcgt gtgcggggta gcgccgatta
    27241 cgtcacttta cgcgttacgt cgcccccgaa aatttccggc catttgcggc gattctgggg
    27301 cgcagaaatc gttttctgat gtcaggggat cgaatcgggc tggcggggtt atctcttcgt
    27361 tacgtggcgt tacgtgacgt tttacgcggg gattacgtgg ggcgtttcgt ccatcttttt
    27421 ttctgctgcg gacggttttt cgtggtgggt gatgtccgtt gatgaatatt ttttatcgtc
    27481 cgggaataaa aaaggccaca ttgcgcggcc tgttaaagtg aatgattatt cgattattgg
    27541 tgaatattta tttttaagct ggcggcactc ctgagcgacc tggctgaact gtgcggcctg
    27601 cggagacgtg cccgtttttg ggtgggtatg tgatgctgta atatcggcca gctcttccac
    27661 cacatcggca aggtccgtca gcatggtcag tacattaatg ccctcgtttc ccagcttcac
    27721 cgtatcgcca accagcttct gcgccagcgc aatactgtga cggacgccct taatcttttc
    27781 ggtaaggctg ccgcccacgg tgtcctgggt gtcgccttcc acgtcgcgct cacaggtacc
    27841 ggcaaccacc atcatgtgcc cgcccgtgct gatgctgtag ttctcttcag tgatatgggt
    27901 gaccgcgcca gcctgaagcg tggatacgcc caggacggtg gttgtgtcgg ttgcctgtat
    27961 ggtggtctcc cggctggtca ggctccgctt ttcctcgtct gcgctcacca cgcgctcgcg
    28021 tgatgtctcc tgtatccgct ggtctgtctg gcgctcccag tctccggcga ccgtcacgcg
    28081 ctgcgatacg ccgtcgcgct gttgctgcaa ctgctcgccc ggcttcacgt cgggcagatt
    28141 gtggccctct gccattatct ggcggatgaa gggcttatcc gcccggcctt ccacaaatcc
    28201 cacttccacc agggttccgg gtggcgggaa ctggaacatg ccggactcac tgcccgccat
    28261 gggaacgggg aggggaacgg cgttatagac gggcgtatct gctgccgggt tgccgttctc
    28321 gtcaagcagc tggagattca ccgcgtagcg gggtctgaac gggtcggcaa tattgcccgc
    28381 gctgacggct tcggtcggtg cctcaacacg tgccagtttg ggcaggtgaa gccctgatgc
    28441 cagctcaggg aaggcctttt cagcctggcg acggaaaggg gacttctgga ggggagcgcc
    28501 ggtagcttta ttgcgcggtg tccacgtgat ggtcatgttg tcatcttcca gcatgaccat
    28561 cgtcaggcgc tgaccgttca cctccacacc cggtcgcagc gtctgaatca tcgggagggt
    28621 catactgtgc ccgcccgttg atgcctggct gaactcgtgc gggatggtga cggggcgacc
    28681 ggaaaacatc ccctgcgcgg cagcagcggc cataatgccg ccgtccggta acggatgcca
    28741 gatgtaatcc ggtacggaga aaatacgccc cagactggcc agcagttcgt agcccgtgcc
    28801 gctgtgtgtg aaatgcggaa cgggtctgtc agtgtaagcc gcgttgtccg gcaggatgat
    28861 ttccagcccg ctggtctcac tcagccactg tgtgaccgtg cgcattgtcg ggtgctgaaa
    28921 tgaacagggg aaggggtgat caaggatccc cgccatttca cgaatcagaa gccgcaccga
    28981 gccgttttca gcgggctggc tgcgttcaac gtatccggta aaccagcgca ccaggtcgtt
    29041 tccgtatccg atatcgagac gaacagggcg cccggtgtag ttctgctccg ttctggccgt
    29101 gataaatccg cgtccgcacg cgttcagctc aagcatgacg gaacagctga ccagctgaat
    29161 ggtgtcgtta cccagtaaca gacgtttagt ggggttcatg gttactccag cgcatcattg
    29221 acgggtttga ggaccttttc ttcgaaccag gtcattttct ccggtgtttc gtcggccacg
    29281 gtcccgcctt ttgcgccggg cgtctgcggc gtctgtttac ggctgctgcc gggtgcggcg
    29341 cgtgcctggc gtttttccgg tacgcttcgt ctttcgcgta atgtgaagct gacctgccag
    29401 gcgaggcggt cggtctgctg ctgtgcgtct atctgcccgg aaaatgtccc ttcacgaaag
    29461 ttaatggcgg cagcggtggc gtttgcgata cggtaaactt tcagggcacc gtccccggtg
    29521 gtggcagagg ccagctggaa aatgcgctga agggcccgct cattgtcaaa ggtgatcatc
    29581 ccggttacgc gaagctcttt ggccttgatg ccctgctcgg cgttcgcggt gctcgatgtc
    29641 tgaccggact ggtccttttc ctggaactgc atcgagggcg aaatcaccat gttctgcatg
    29701 aaaataccct caccatcaag ggcgagcagt gcggtctggc tcatggatca ttttccccag
    29761 ttcggttaat gagtctgccg ccaggagtgc ggccacagta tgtacggcgg tcggtgccgg
    29821 gatatccttc accatggccg ccgctgcggc gcggtgatac ccccggtact gaaataccca
    29881 ggcctgcgcc ttctgttctg ccagtgttct gatcccgtct tccagtccgg caagtgcgcc
    29941 agcccgtctg ctgataaagc ggcttatctg gcggcgaagc cctgcggcat ccgggcgttg
    30001 tgctgccagc tgcctggcct gtgctgcacg ggcggcactg actgcgctgc gcattgtggc
    30061 aacggaaaga tgcacaggtg cgggcagggt gtcggtggtg atcgggattt gcattttgtc
    30121 agtgctcaga ctggcggcag cctgtgccat gcgctgtgcc tgcgtgaata ccggcacggg
    30181 cagaacggcg gtcagtgccg ccattgactg tataaaaaga tcgtgggtgt tttcggcaat
    30241 catgataatg gtcacgtcgc cggatgcacg gcttccggcc attttcccgg ccagccagtt
    30301 tatggcgttc ggagggctta aatatacccc cgattcttca tatcgtccca gcccgtaagt
    30361 ccacggtgtg acggggatga cggaacagct cagcgggggc atgtcagcgc tgacgctgat
    30421 tgtttttctt gtccacgtca tggtgcctcc ggtcagcctg cgtgatgatc cggcgaaggg
    30481 gattcaggcc agacaatatc cggcgcgttg cccgtgtctg tccggttcag taatacccgg
    30541 tattttttcc acaggggaag cagggcgctt tcatcgtcgg tcgccatatt taaatccacg
    30601 gcatcctgga ggatgtttat tgtatttgtt gcttcctgta ttaattcccg ttttttgttc
    30661 tccgcctcgc tcacccggta agcctgttct gctgcttcat ccttaaccca tgatgaacca
    30721 tcccaccggc agaactcccc gtccggtttt atttctgtca cgttttccgg taacgggccc
    30781 ggctcagtaa tcagtaaggc gctccccgac tccagacgcc agacggcttt accccggtga
    30841 tcttccatga ggtgccaggc gttttcatcc tcatcaaaga tagccacgtg tccttcagga
    30901 atgtcaggcg gggcaatgtc cgttgagtgc gcaggcagtc cggtatgcgg agggatccag
    30961 gcatcgcctt cgccgataaa ttcatttgtt ccggctgaca ggttgtagat tttaattgtc
    31021 tgcgggtgtt cgctcatttt aaaggtcatt atgcaagcct cacgatgtag ttaaatgcga
    31081 tgtttttgac ggtgttttcc gcattaccgg tggggttaat ggtcaggccg tggccatgtt
    31141 gacccagtgc gagcgtgtgg gtgtgttcgc caatgcttac cgaatgtgtg tgttccgaaa
    31201 ctctggcgat caggttcgcc ccggagcccg gcccgtatgc ggtccagtca ccgctgcctt
    31261 ccggtgacgc ggtggcggtt cttaatgata ttgttgcgct gaacggcccc gcgctgtttg
    31321 ttgtttttgt gccgtaattg aaggatgagg tcgtttttgt gccaaggtca gtatttgaga
    31381 cgctccctcc gtgggtgtgc gatttgatgc cgtcctgctc ctgtgacaat accgcacgac
    31441 cgctggcagg ttttcccttg attgtccagc cacgcatatc cgggatgcga ccgtccggat
    31501 aggcttttgc cagcagggga tattgcgctt tatcgaatgt ctgccccacc atgatggcgt
    31561 aaccggcagg tggggtgtct gacggccacg gcaggggaac acctgcgggt aacaactcac
    31621 ttgcaggcag tgcgccgatg tcctgtggtg tgggtttgtt tttgtccgtg taagtgcggt
    31681 accagatggc gcttttccct ttacttaaaa taactccggt gaactgtgtg cggtgaggtc
    31741 tggtgatgtc ttcaggggat tcaatgcctg tagtgatcct gatgagcccc ccgttgatgt
    31801 cgcctgatac ttcgataagg cagcctccaa ggcagatatc gccatagccc gtatcagtga
    31861 gcacgctgtg ctgtgacagt gaaatattgg ctttgaatat ccagagtcct ggtctgaagg
    31921 cgccacgttc attcagccat tccacgaatt gttccgttgt ccagcgttgc cctgctttac
    31981 ccacgtaccc gtcttttgca taaacgcgtg gtgcgcccac gttgatcagg aacagctcct
    32041 tgttggggat gtcctcacca ttgcggtttt tcgcaagccg accatcggcg ttgtccatgg
    32101 cgatctttat agcctttggc gtggctgcct gtgtctctga tgtactggtg gtggcgctgc
    32161 ttaactgtgt gaagcccttt tctttcagcg ttgcgtccgg gtggttgcgt gatttctcgt
    32221 gttttttcag ggcgtccaga ctgcggcgct cttccggggt tcctctgggg cggaggtcag
    32281 tcacgttgcc gtcttcatca atttcagcga tggcacaaaa atattgctga atgccgtccc
    32341 tcggatacgt tttccgtaaa cgctcttccg taacgtccgg gtcggctgtt atgatttcgt
    32401 gctctgtcgc ccattcactg gtgagcgtgc cggtccacca cacattcagc cagatacggg
    32461 acgggaaggc gggcgtggtc atggggtggt gagtgctcag ctctgcgcgt agcccctcca
    32521 cgtaacccac ccctttgttg actgtccagg tttcgccttt cttaaccagc cacccgtcat
    32581 caaaaaaagt tgccttcccg tagatatcga cgttttcgcg gcgctggcgt tcgtccatgg
    32641 atgccaggcg tgccgtgaag tctatctgcc aggtctgcgc cggggtggtg atacctgtgg
    32701 cttccttcgc accgctgtac tccatgagca tggaacggac cagcacatta ccttcgttac
    32761 cgttgtgggt tctgattttt ttctgtgtgg gggcgtgaac aatcatggcc agcgtgctgg
    32821 ttgctttgct gaccagtccc acccagttaa attcaaaatc acccagctct gcccccaggg
    32881 tgaccgaata aaccacggca ttctcattca ccacgccggt tttgccaacg gcctggcggt
    32941 gaacaatctg atcttcaggt ggcagggttt cgtcggggga taccggtgtt tccgggttga
    33001 ggtccgggac gtttgcaaaa acaaactcat ccagcacaac gggggtgcct tccgccgcct
    33061 gctgtgcctt gagccggata aaggcgctgg taattacaga tttcgccata aagtcatact
    33121 ccttcagtca ttcactctgc cctggccgtc cagcagatat actccccttc gtatgcgcca
    33181 taatggatga tgaaggggtt aagggtttcc acctcaaaac ggtaacggcg gcatgtcctg
    33241 ccatatttgc ggatgatctc cagaagaaga tcgctgttgt cggctatctg gctgtctgtc
    33301 acctgcacca ggatcacatc ccagtccacg ccgggcttgc gctcctgcaa ctccacatgt
    33361 ccaatgccca gccgttcaaa aatggcaata aatcctgcca cctctccggc gtcctgtgca
    33421 ttgacaaagg cgtgggcgat acggcggcgg tacagtgatg tgggttcacc tttaaaacgg
    33481 gtgatgtccc gctcccatgc cagtaaatca agtacgggct ctgcacatgt catggggtca
    33541 aactggcgca cgggccaggt gatccattcc catacgcggg accagaacgc ctgcgcggcc
    33601 cgcagcagtt tgtcgggttc ccccctgtcc atccaggagg gcagtttcag cgagcgaagc
    33661 cgctgcaaaa agtcattcaa tgaccacctc cactgtcagg gattcgagcc gtggcacggc
    33721 cagatcgctg atgatgtctt ccagtgaaaa ctgaaccgaa gccaccaccg ggaaggtctg
    33781 atgaatttcc ttgcccagcg tggaaaacga aaagcgcgaa tagggccaga cttttttcac
    33841 gttaaagcgg gcgttctccc ggaaagcgca gcgtatcagg tcgccgatat cctgcttcag
    33901 cccttccgcg ccgtccggtg gcagggtggt ggaacttctg atgtatacgg tgacctccag
    33961 acggtgacgc gtctccggca tggcgaaaca ctgcaaatca tccccgtgtc cgtggtgccc
    34021 ctcttcgttg atgtagcggt tgactgcttc aataaaaggg gcagaggcct cgccgctgtc
    34081 cagtaacagg taggcgttgg ccgttcccgg tcctctcggg gcgtcgtgct gaaagtagat
    34141 ccggtcgatg ctcaggcccg ccacgctggc gataagtccc cggtagacgg cgtccgtgtg
    34201 gtaactgctc acgaggttaa actggttgcg tattctgtcg cgcagttcgt cgtcgctttc
    34261 ctcatcggca cccggtacgg tcagccagtc ttcccggttc accacggatg caatgccggg
    34321 aatggcaacg ggaaggatac ggaagtaccc cggcgcgagg ttgtgcccgc tgccttcggt
    34381 ggtggcttcc acgggaacgg atgcgccgct tttaccgctg ctgataagcg tttcctccgt
    34441 gacggccatg ctgtatatca cgccgttaat tctggcggtc tggacgatgg tcccttcggg
    34501 gatgaggacg tcatcatttg ccctggtttt ggcaaaccag atcacgccac tggccttact
    34561 ggccggtttt ggggtgatgt tgacgtcgtg cgcatgaagc agaagaagtg accggtcagc
    34621 cgtggcgaca aacataccgg gcagcacaac gtcaaccagc gcatcacgca gccactgaac
    34681 gggtcgtatg acaatggtca gaatgagccg ccagaaggga ctcatgggcg aggtgttggt
    34741 gatgagtccg gttttctgta catcctcatg aaagagggtg gcgatctctt cttctgtttc
    34801 cggcataccg ctttcacgga atacctgatt aaaatcaacg tccggtttcc ctttcatagt
    34861 tcaccctggt gtttatgcgt ccaaaatcct gtgtctctgc cgtcacatgc agcctgcccg
    34921 gtgcctcttc gttaatgaac accgtccccg gaaccagccg ttcatcatcc tcaaccagga
    34981 ggataagccg ggtaatgatg tcgctgcgca gcgtcgggct gcgttcgccg ataagcaggg
    35041 cggcgatccc gctttcgata atgctgtgca ccacgtcctg gccgatgctg acccggttat
    35101 cacatcgctg tggctcgttt ccgctgttca gcgtaaaact gccgtcagtg ataagcaggt
    35161 cgatgtaaag cgatggcgtg ttcatcccgc gtaaagctcc gtccattcat ccagttgccc
    35221 cggcgtcatc gggttctgcg tgttgattgt cagattgccg aacgtccggc tgttgtcgat
    35281 acgggttgag gtgtccgtgc gtatctggtt gcgtatgcct ccgggtggca gctctgcgct
    35341 gatgcggttg cctgccagtg ccgggacggc ggtttctgca atctgcggag gtacggtgct
    35401 gcctgttgcg ccccgcgtca tggtgttcac ctgctccgtg ggcttcatgt cgatactgat
    35461 accggggatt ttgttcagct tttccgcaag ccagttccac gtccggttaa acgagttttt
    35521 aatgacgttc cataagtccc cgaagacgtt accgatgatc cccgccattt tccggaagga
    35581 ttcgaccggg gcagtcgggt caaatgcagc caccacgtcc tgccaccctc tgatgacgat
    35641 ggcgaagatg tcgaccaggg ttctgatcac gctgataacc acctccagcg cgggggatat
    35701 caggcttacc gctgcggcca ccacgcgacc gcatgtttcc ccggcacggg tgacgctgtt
    35761 catctgtccc gtggtcagct gaaccggtgt gaacagatca gtgaaggcgc cgaacagggt
    35821 ttttatgccc tgccataccg gggccagcgc acgggcaatt tcaccgaacg cggcagtcac
    35881 cgggctcatg gcgtccagtg cgccggtaaa accatcaata aagccgcgaa taaaggcttt
    35941 gacgggctgc cagaacttaa ttgccaccac caccagagcg gcgatggcgg cggcaaccag
    36001 gaggaccggg gcgctcatgg tcagaaacga cacgcccgcc agacgcgcgg caagactggt
    36061 ggcaatgatg gcgctgcgta cttttgtgag tgcgagcgtg tacagactcc agactgccct
    36121 ggcgcccttt atgatggcct tatggccttc catgatgaag ctgaacacac ccagcacgat
    36181 gttcgtgacc gcaccggctg cgcccagcgc cagcatggtc agcgtgatgt aacccagcca
    36241 gcgggcgatg ttcgggaaca tgttcagcca gcgcacgaac gtcgcgccga tatcggcaaa
    36301 acggccagcc agtggtgcga gaatggggta gagggcgtta ccgatggcgg cccggatgtt
    36361 gtagaaggag gccacaaggc ggtcccacgg gtcagtcatg gcttccgcca tttccgtggc
    36421 acgcttcatg ccgtcattac gccccagcgc tgatatgctc cggttaagca tttcctgctg
    36481 tccccagagt tttttaatca cgtccgcgcc actgccgaag gcgtcatcca gcgcctgttg
    36541 tgccgccacg ttaccctcga tgttcctgcc gaacctcgcc tgcagacggt cgaggatgtc
    36601 acccatgggt aaaatcttcc cggtggcatc gacaaaactc atccccattt tttgtgcggc
    36661 agcgggggca ctgcgcagga actcctcata gatgcctgat gcctcggtgc ccatggtccg
    36721 tgacagggtg cccagtatgg caaactgctc tgccatgctg acgccaaaat ccgcgcccgc
    36781 gtttttggtg ccctcaataa gctcctgcat ggtgctcatt tccacgccga actgctgaac
    36841 catccacgcg gttttacctg ccagatcttc agcaaacccc acggcaccca tggcagaaaa
    36901 ctcactgcgg aaacgggcac ccatggcggc aaggtagccg ctcgcctctt cgctgctgac
    36961 gcgggcagcc acggccaggg tactggttgc tgccgtcaca cggggtaaat ccccgtcgct
    37021 cagcccgtcc agtgcgccgc gaatatgcca tgcggaacgg atgatctccg tggaagactg
    37081 accgaatgcg gtccccagtt tcatggcctg cgtggtgagg gcggtcagcg cctgcgctcc
    37141 tacgcctttg gcctcaagct cgcccagcgc cttctgcatc tcccacgccg gatccatcgc
    37201 ggcgcggata ccttcggcca cgccccacag tccggccaca cccacaccga tacgcgcgaa
    37261 cgcgccctgc gagcgttcgg cgaatcccgt cacggattcc tgaacctgcc gcagcgggcg
    37321 ggtaagctgg tctgtcagat tgataatgaa atccagctgg ctggccatgt caccttccgt
    37381 taaatgcgat cgctatacct tcagcggtct tgttggcgcg ggtacgggca aaatattcat
    37441 ccagccagat ggcacgggcg atgctctctt cgtcgtccgg ctctccgggg aggtagtgac
    37501 gccgcaatgc cagatactgc tcaaggggat tgcggcggat gctttcgacc cgcgcggtca
    37561 gttttttaac tcaatctcca gtttcgggga gtaaacctcg ttaatcttct cggcgatctg
    37621 catctcgcag ccggggtaac gatccatgag gtcggccagt gcatcccggc agtcagcggt
    37681 cacgatacgg ttaagatagg tggtcattgg cgctatcttg ttggtcatcg tgatttcgtt
    37741 gatgaggttg ttaaatgccg tcttgttcgg ctcaaaggtc agggttacgc ccgcgacggt
    37801 cagggtgatg gtgccggtgc tcttcgtggt cattgcagtt tctccgtgtt tcgtatggtt
    37861 tccaccaggc tgttatgcct ggcggcgcat tcggtgtagc ggctcgtgag ggttcgcagt
    37921 gccgcatcaa aatcagcgcc tgtcgggcct gccagtctcg gcagtgtctt cgggcattcc
    37981 gtcagctggt tttcctgcca gggtacgttc agcggcttca gcacgggcgt tgaacagccg
    38041 gacgtaatca tcagtagcgc acacattgcg gaataccggg cggacggttt ccgtgtgaat
    38101 aacgcgttca gtgtgaattt cattgccctt taacgcctcc agccgttttt ccagtatgcg
    38161 ggcggactgc tgcccggctt ctgtcaccgc ccgtcgcgtg gcttccatgg cttcctgttc
    38221 ggcaagcgcc accctggcgt catgccagtc atgggccagc cagcccgccc acatggcggc
    38281 aaagaggatc accaggcagc ccgccagctg tttcatcatt tcaccccgtt gtgctcaagg
    38341 ctgaaatgat tgccgtctgc ccggctgaag cgcccgcccc atgtgccgcc gatggattcc
    38401 cagtattccc ccagcggcct gtaggcgtcg gtgtcggcct ggtatttgcc gttaatgaac
    38461 aggttgaagt ccaccgcgag gcgcagcgtg tgaaggctgt tacgaatgcc ttttccgctt
    38521 ttcgcattca gtgcggcctg ctccggcgtg cggtatgcct cgccgaaggt caggcgatag
    38581 ccgtgctcct gtgcccagtg gatgagatcg gcaatcatga cggcaaacag ttgttgtttt
    38641 tcgctcagtt tcatcagtca ttcctctgtt ccgtccccgt aaggcgcgcc tgtgccacgc
    38701 tgaaatagtg ggcgttcagt tcaatacccg taaaacggca gccccggcga agggcggcca
    38761 cgcccgtggt gccggacccc ataaacggat caaggatcag ccctccgggt tcacacacgc
    38821 gcacaagctc atccatcaac gattccggtt tacctgtcgg gtgctgtttc tcctgtgggc
    38881 gtgtgtggtg cgtgatgcag ccgtcgaacg gccctgaagg ggatttgccc agcgcgcctt
    38941 tgctgcccca gaccacatat tcgcactggt gacgaaaata accggtatgc ggtgttcttg
    39001 agccccgccc cttgttccat gccacgatcc cccgccaggt aaagccggcg gcctgaaata
    39061 cgtccgtcag cgcgggcaac tgccgccagt cggtgaagac cagggcgtaa ccaccgggtc
    39121 gtaccgtgcg ccatgcctgt gtcatccaca gatgcgtcca gtatccccat gaacgggcgt
    39181 ccatgttctc gccttcgaag ccttcaaagc ggcgaagatc ttcatcgttg aggtatttgg
    39241 cgtggctgcc ctggcatgtc cgccccgcct tgtgtgtggc acctgaactg tatggcgggt
    39301 ctgtgatgag cgcgtccaca ctgttttccg gcagcatccg cagaacgtca tgcgcctgcg
    39361 cgtgatacag gctcagggaa ggcgaaatgt aaggcttatg ggtcatttct gcgttccttc
    39421 tgcttcaggt agcggtctgc cagacggcaa aagcattctt caataaaggc gctgcccata
    39481 atgcccagcg cgcacgccag accgaccaca accagttccg gcatgtccgg aaatttaaac
    39541 agcggaatgg cggcaaccgg cgccacggca gagcccataa tcattcgccc gataagcaga
    39601 cgggctgtga tgcgatcgcg gctcaccatg agacgcccca gcccgatggc agcaccgatg
    39661 ataatcagtt ttgccaggag tgaagtctga ccgtccggca tggcatcacc ccttcagatc
    39721 gcgggtgtca cgggctgaca ggtagggcac gccgtcaatg ctgataaagt ccgggcttgt
    39781 gaccatgaac ttgatttttt tcgtggtctt tccggcttca cctgcgctga tgttcaccac
    39841 gtccgacaac atggggacgc agccgaacac ctcgattttt tcctcctcgc tgccgttgtt
    39901 ggcataaaag agaaaatcct tttcaggaat ggcacgccac gaccccgcag agcgggccac
    39961 ggcaccaaac tttttaaagt tctgcgcgtc gacttccacc tccacatcgg cagaaacaga
    40021 accacgggtg tagccgttcg gcacgccgcg cgtctgtgcc acggcgctgt tgtcggtgat
    40081 ggtgacggtg gcgctttcca cgtggatcat gatcccgtcg tagttgatat caaacgcgcc
    40141 gccgctgata cgttcatttc cggccatgtc agatctccag tgctgtgtcg agttcaatac
    40201 ttacgccaat ggcttttgcg ctctcgtagg ggcgtaccac aatgtaaatc tgcacgtgtg
    40261 tactgctggt ccagacgatg gtgatatccc cgtcctgtgg cggtttaacc tcgccgggga
    40321 acggtacgcc gttaatctcc gtggcgagcg ccatttcacg cagcgtgcgg ctgaaatatg
    40381 tctggctggc ggcaatgctg cccggtgtgc tgttgagtgc gcgatccgca aggcgggcaa
    40441 tggcgcgcag acgtacacgg cgggagacct tatccaccac gcgcacgttt tcgatgacct
    40501 ggtaatcgcc cccctccaca tcaagggtgc gtccgtctga ccagtagagc ccgtcgaaat
    40561 cgtgatacca catgggcacg ctgaagcgtt tgccttccag cgagcgcagc gtggccagtt
    40621 caaggggctg tttcttgccg tcctgcggga tctcatcgtt acccatcgcc accagggcac
    40681 cggttgccac acgtgccgga ctgtccgcaa cggtgacgga aggattacag agacggcccg
    40741 ccagtacgcc gggctcattt ccccataagc ggggaaccac catcacgcca ggtgctgcca
    40801 caccgtcctg tatggcactg atgcgggtga cgtatgccgc ccatgcttca tccttgcccg
    40861 gtccggccac gcacagcatg aaccagacaa aacggccata agcggactga agcagggcgc
    40921 gggtttcggt ggcgcggtta acggcatctc tggccgtgat gtcaatggca atcaccacgc
    40981 cttcggcaga ggcgatggtc tgcgcttttt tcaccgcatc cacccatgcg gcgttagcgt
    41041 cgccgccctc cggtgccggg ggtttgggtt cagacagaac gtgaacatag gcaaaccagt
    41101 tctggccacc gttggcgatt gcggcggtaa gcgtggattt aagcaggctt tcatccggtc
    41161 ccagcacgtc gtccaggtcg gtctcagggg agacggaaag ggtgttacct gtgttctttt
    41221 tgccatagcc cacaaaaagc agcgtgcgct ccacttcacg ggtggcaccg ttgaaacggt
    41281 tctgatggct tacggttaca ttcggccagc tcacggtgtg actccttatc tgatatcctg
    41341 tgcgtcaacg tccaggccgt agcccattgc gttcatctgg cgtgccagga tgtgtgaaaa
    41401 ttcttcgttt gttaccccca gaaagacgcg cgacggaagg gtaatggtcc atgtccttgg
    41461 gttgttacgt tgtcgtattc cctgaccatc actgctgtaa aggtcgcgaa aatcttccca
    41521 gtcgtcgcgc tcaaatcttg ccagtagcca gccagcctgt cggttagata cccttctccg
    41581 tatcattgac agggtggctt ttcgccagcg cttgccgctt ttcagctcaa gcccccacgc
    41641 tctcagcgcc tgtgcctgct tcagggaaca cggtacggaa cgcgccggtt ttttgtgccg
    41701 gaagctgtcg ccgtgatagc ggatggtggc accgttgtga tgtgcccacg cgacgttact
    41761 cgccgacgct gtcggtccgt atcttacagc gcggaaatac accttcaccg cgtcctgccc
    41821 cggtatctcc cgcactgcca taaggcgcgg gagtttttta agcatctttc cggtgttctt
    41881 gcctgccttc ttttttttgt ttttccgtgg tgcccacggc gtgccgtctg gtgcgcactg
    41941 gttgcgctga tggcgtctgg ctgcggcgat aatgccgtgc cgggcaatac gccataacag
    42001 tcgctggcgt tttttcgggg gcagatccag cgactgaagc atcgcctgcg cttctgccag
    42061 ttgctgcggg ttcagctggc cgcttatcat tcgcggctcc tgctgacaaa cagatccatg
    42121 gtttccgccg tccagatttc agggttaacc agtgtccagc gtttccccct gtacggaacg
    42181 gggccgtctt cctgcacccg gatgatcagc gggtcagcca gtcccaccgt gatatcgaga
    42241 aggcacgagt cctcatcgtt aaactgcggg tcgacgttca catcctccgg gcagaattcc
    42301 tccctcagcg ggtccgcgtg ctcttccagc caggccatga ccagcgcgta aaccagcccc
    42361 ggtgagtatt tgcggaacgg gaagttatcc catgcaatca cgccggtgta cgtcatcacg
    42421 cccatgcgga tctggtcgtt acccagtgcc ttctgacttc ggataagctg cgcgtcttcc
    42481 tgactgctca tgaagaactg catggcgtca gacggcagat tttcggtaat gaaggcggtc
    42541 aggctttcca gctggttcat atgatgtgca ctcccgcgcg tctgtgcccc agcatgttac
    42601 gcatgaccag cgaggcttca gccagtaagg tggcgcgtgt gtcctcgctt tcctgccccg
    42661 gataggtttc acggcgacca atggtggcga actcgcccat aagatcggcc ttggcacggg
    42721 cataaacggc ctttttgtac tgtgcgacca gctgggtttc atcgcccagc ctggcccccg
    42781 gtacgtctgc ggcgcgttca tgcccctgcg ccttccagtg caccacaacc ggcgccagcg
    42841 tggtgttcac ctcggccact gcggcaagca gcgccatggc cgccgtgtcg tcgggtaaat
    42901 cagccggaag cgtgcgtgac ccctgaagct cagccagatc aaggtccggc cagaacggct
    42961 cgccgtttgt caccgtctgc ggtgtggatt tcagcggttt accgctgacg ctgaagctcg
    43021 gttcgctcat gacgtgcctc atatgaataa aaaggcgggc tgtccgggat tccacggcca
    43081 tacagtttcc gtcactgatg cctcctcccg cgcccgcccg gccctgcggg tagtcgttta
    43141 cgctgcatta agggcgctga tgcgtgccct gatgcgcgcc ctgatacttt ttacgccagc
    43201 ggtacggctc agtgctgccg cctgtgccag gtatgcgtct gccttttcca gtacggcagt
    43261 atcattaatc gcggtggctc tcggtttgcc cgcctcgtcg cgcagaagat acagcccggc
    43321 aaatctgaac cacttcgcgc ggatttcctc atgcagccgc cacgtggtgg tgacgttcct
    43381 gaaggtgcgc gaaaaatacg gctctatgct gtgtccggcc tcgctgttgt cggtggccca
    43441 ctgcagcacc gtgtcggcca cgaaggcggg cagggtgctg cggaagttgt ccggcgtggc
    43501 ctggccttca ctgatggcaa tgtctgccca gtcaagggcg cgactcaggt cgcccgtgtc
    43561 gaacagccag atgatgcagt acaccagtac cgggcaggcg tggcgctttc cgtcagcgat
    43621 atagcgctcc gcgacgggca gccagcgcgg caggagaatg tcgcgcttga acgccagccg
    43681 ctcttcacgg accggaagac tgcgcagccg tgccacatcg cgatcaagct ccgcgcgtaa
    43741 caggtggaag ctgaccgggg aggccgtcag cgcctcccgg cgttcgagtg cctcctgtgt
    43801 gcggattttc gcccggtgtc gctggcaggg tgtcattgcg ccgccctctg tgcttatgcg
    43861 ccgccagatg cgggctcttc tgccgcttct gcgcccttga tggtcagctt gtcgtaggcc
    43921 gcgtaaattt cgtcgtgctc cacggcgtaa ccttccatgc gcagatagtt gttttcaaaa
    43981 cgcttgcgat cgtcgttcca ttccgcacgg cgctgacggg tgccgcgctg cgtgtagatg
    44041 tgaaggtttg ccagcgtggt gacaatcagg cgcccttccg gcatgaacgg cggggtgtat
    44101 gctgtcagcc cggcgatctg gcgaccgata agctgcgcgg ccactttttc cgtgggggcg
    44161 tcaatggcgt taagcagcgt caccttgtcc tgtgccacaa gatcgggcgc aaccagtacc
    44221 accagatccg gatgaccgcg atacgactca tcaatgaggg tgtgcacgag gtctgccacg
    44281 gctgcatcca gcccgataaa gtcgccaccc ttgcccagcg tcgggccagc ctccacgatc
    44341 tgtttttcgc tgcggccttt gacgatggca tgccagccgg tgttgacgtc ctcgccgttt
    44401 ttgttggttg ccgggtcggt atcttcggcc acgctggtac cgttaaaggc gatgcgcagg
    44461 atatccagcg cgatctgtct gttaacaaaa ttctgaatgc ggttaaagaa ctcgttttca
    44521 ccgcctgcgt ttgcccaggt caccagtgtg gtgtacagga ggtgggcgcc ggaatcggtt
    44581 tcagccagct tgtattcatt cccggtaaca cccagcggaa gggtgaagcg ggcatttttt
    44641 ttacgtccgg tatggaggcc cggaacgccg gtggtgacca cctggcctgt cacctgttca
    44701 acatccagca cgttcggcag ttttgataaa aactcgtcgg agtgcatgat ggcgtcacgc
    44761 agcgcggtct ccttcgggtc agacagcgca aaatagcgtg tgacgtcgtc aaccccgttt
    44821 gccgcgccca gcccggcagc gtacttacga agcagtgatt cggttttctg gttcagttgc
    44881 atgttgcgtg tccttaaaca aagtcaaacg gtttttcgct ttctgccggt gcgccggccg
    44941 ggcgtggcgt gatgccggtt gctgacagct tcgtggtcag gtcgttaagt ttttcggcaa
    45001 tagctttgat ttcttctttg tcggtgtcgt cccggcgacg ggcggcaaag cggcggtaac
    45061 gttcgccttt ttccggtgtg atggcactga acgctttaat cgcgtcttcc agcttcgctt
    45121 tcgcggcgct gaaggcttca gccttaacct catcttccgg ggcggcttcc acttcttctg
    45181 ccagctctgc cacctctgcg gcggtatcgg cgatttcggc ggcgatttcc gcgacttcgg
    45241 cttttgcttc ttccggcgtg tctgctgtgc cctcgccagt ggcagcggcg ctgccgcctt
    45301 ccaccagggt gatcagcttt tcaagcagtg ctttaatttc gtccattttt gcgtcctcgc
    45361 ctgtatcagg cttactggta tcggtgttgt cggtctgtcc tgcgctgaac aggcgtttca
    45421 ggaatgattc aggtttacgg ggggcagggg aggtgtccgg tgacaggctg cccagcgtga
    45481 acgtctggca gttcgccctc tgtgccgtgt attcctcgtt gttcagggtg aagcggattt
    45541 tttccgtccc cagactggcg gggatatccg tcaccgccac gcccaggaga tagtcgcgtc
    45601 cgctccgggc gaagttctcc actatctcgg cggatgtgaa cagcttctgc cccagtctgt
    45661 tggcttcaat caggtactgg ttagggacca gccgtgcgta gaggcgggtt ttaccgtcct
    45721 cgccggtttc cgctttcagt gagtccactt cacccagatt actggtgtac tggcggtgtc
    45781 cgatttcttc ctcatgccag ggccacagca gcgccgtgta agtgttgcgg gaataggttt
    45841 cagcggcatc agtcagccac tgcggatcaa tttcgcggcc gtcaacggtc ggtcctgatg
    45901 tggcgataca tatccagtcg gtcatcaacg ttggctgcgg catcgtggcc tcttttacgt
    45961 gaaaatctga agaactccgg tgagtgtcgg gatttttaca gcggggggct actggattaa
    46021 ttcggttgca ttcggataac acgggatagc cgaacacagc cgaaattttc tgcctgaatg
    46081 cgggccggat gcgccgccat aatgggcgca tggctaaata ttctgacgaa atcaaagagg
    46141 cggcgcgatc gctttacctg aagcggtggc acccccgcga tattgcggcg gaactcggta
    46201 ttccgccgcg cacggtttac cactggtgtg atgtggggca gtgggctacc ctgctgcccg
    46261 ctgaatccgt tgaggacgtg atcgcccggc gtattgatgt gctgaccgga cgggagggca
    46321 agacagagtg tgaacttgcc gaactgcgtg aacttatcgc acagcacgtg aagatcatcg
    46381 cccagaaaaa caaacacgct gaaaggatgg cggaaattgc cgccctgcgt ccggcagggt
    46441 atgaggacgg cgcggctgcg gggacctgcg gcgggggaaa agggaaaaag cggcgctacc
    46501 ggaaaaacga cgtttccgga ctgactgctg aaatgctgga tgaatgggcg cgtaaaaatc
    46561 tttatgccta ccagctgcac tgccgcgaga accggcaccg tgagcgccgt ttcatcctca
    46621 agagccgcca gatcggcatg acctggtatt ttgcatggga ggcgttcgaa gatgcggtgg
    46681 tgaccggtga taaccagatt ttcttttctg cgtcacgggt acaggcggaa atcttccgtg
    46741 aatatattat taacttcgcc ctgcagttcg ggatcacgct cacgggtaag catatccgtc
    46801 tgagcaatgg tgcaatgctg cgtttcctgt ccaccaatgc cagtaccgcc cagggattta
    46861 acggccacct ttacggcgat gaggtgttct ggatccccaa attcacgcga ctgcatgaag
    46921 tggcctcggc aatggcgacc cacgacaaat accgcacgac gtatttttcc acacccagcg
    46981 ccaaaaccca ccaggcttat ctgatctgga atggtgacga ctggcgcggg gatgacccgg
    47041 cgcgtcgtgc tgttgagttt ccgaaagaaa gtgcgatgcg tgtgggctgt gaatgccctg
    47101 acgggatctg gcgctacatt atccggctgg aagaggcggt ggcgggtggc ctgtctgcac
    47161 gggttgatat tgaacgtatc aggaacctct acaacccgac cacctatgcg atgctgtacg
    47221 gctgcgagtt tgttgacagc aaggatgcgg tatttaagtt ctccgaactg gtcaggtgcg
    47281 aggtggagat ggagacgtgg ggtgactacg atcccacggc ggcacgtccg ttcggcaatc
    47341 gtgaggtgtg ggcgggcttt gacccgtcaa ggtccgggga taattccacg tttgtgattg
    47401 tggctccgcc tgtccatgaa ggcgagcggt tccgtgtgct cgccgtctgg cagtggcagg
    47461 gatttaactt cacctggcag gccgatcaga tccgcgaact gatgcagcgc tttaacatta
    47521 cctatatcgg catagatatc accggtatcg ggaaaggggt gtttgatatc gtcagtcgct
    47581 ttgcgccacg tgaggctaac gccattcttt acagcgtgga gagcaaaaac cgcctggtga
    47641 tgaaaatgat agacgtggtg gcccataagc gtatcgagtg ggcgaaagac gccattgatg
    47701 aggccagccg tgaacgcacg gagatccccg cgtcgttcat gtccatacgc cgcaccacca
    47761 caaaaagcgg taacgcgctg acgttcgtgg cggaacggtc ggatgttacg gggcatgcgg
    47821 atgtgttttt tgccatttcg cacgccgtga ttaatgagcc tgtagattat gactatgaac
    47881 gcccgtccac atgggccttc gggaaagccg catgagcaag aaacgcagaa acaaaacgtc
    47941 acgcgtgacg aaaaaagcat tcacgcccgc acgtggcagc acaatcacgt ttggtcagcc
    48001 ggaaccggtg ctgaccaccg gcacggacta ccataacatc tggtatgaca acgaacacaa
    48061 ccactggcag ctgccgatta accgcctggc actggcgcag ctcccgaacc ttaacgccca
    48121 gcacggtggg gtgctgtatg cccgccgtaa catggttgcg gggggctata ccggtggtgg
    48181 cctcacaacg gaccagatgg aacaggcggt atttgactac ctgttgttcg gcgatctcgc
    48241 cctgctcaag gtgcgtaacg tattcggcga cgtcgtggca cttgagccgc ttccgtcgct
    48301 gtatctccgg cgccgcaaaa ccggtgagtt cgtggtgctt caggaaggcc ccgcgctggt
    48361 ctatacgccg gaagatatta tctttctgcg gatgtatgat ccgcgtcagc aaatttacgg
    48421 gctgccggat tatatcggtg gcattcactc ggcgctgctg aacagcgagg ccagcctttt
    48481 tcgtcgccgt tactacaaca acggggcgca tatggggttt ttgctgtata cgaacgaccc
    48541 ggggatcacc acggagatgg aggatgagat ccgcgagaag gtggcggagt ctaaggggct
    48601 gggaaatttc cgcaatctgt acatcaacat ccccaaaggt tcgccggatg gggtgaaaat
    48661 tattccgatt ggtgaggtga gcgccaagga tgaattttca aacatcaagg ggatcagcgc
    48721 ccaggatgtg tttacggcac accgctttcc tgccggtctt gcggggatca tccccacgaa
    48781 cggggccacc atccctaacc cggaaacgac gcgtgaaacg taccgcaagg atgaggtgat
    48841 accgcttcag cgtaaaatca tgaacgccgt gaacagcgat cccgatatac cggaacacct
    48901 ttatcttcgg tttgatgtgg aagctgggca tgtggcggaa gaaaaaacgg tgccggtcgg
    48961 gtaaaatgtc ttccccggga tacgtgttat cgggaggtaa gatgcggatt tttaaagccc
    49021 ggtgtcctga ctgtggcgca cctgctgtca tccggaagac agactggaaa acccctgaac
    49081 ttgcggatct gtactgtgca tgcagtgatg tcgaatgcgg gcataccttc gtctgtacga
    49141 tgaccttttc ccataccctc agccccggcg cccgctccgg tcgcaatatg gtgaagtttc
    49201 ttgttgatgc catgcgcccg gaagatcgcc ggtatgcgct tgaacttctg tcgcggcgtt
    49261 cctgacgcgc ttcgcttgtc tgctgcttcg ccggggcaaa aagaattttt accccgactc
    49321 accagctcct ttaacggaat gcagcgccgt agataaacag aattcgcttc atgatttcgc
    49381 tgcgcttgca ggcttcgatg actgattttt cctgctgccg ttcagcccgt tgcgccagtt
    49441 cctgtcgttc cttttccgtt ggcttccggt aatgcgttat cttgtccgtg tacccgtcca
    49501 caaccagcaa cccttcccgt accatttttc cggcggcctg attaatgacg gttcttttca
    49561 cgcccagttt ttctaccagt tctggggcgc taaaacgtgt gtgcgtttcc aggtagcgta
    49621 aaatgatttc ctttccggtc attcctgggg tcttcatttg gcggctcctt cgttgttccg
    49681 gcgttcccgg agttcggtca gtgctgcaag taaattctcc caccacgttt cttccacgac
    49741 cgggttttgt cgtgaaagca gctgctgcgc cgactcaatc attttcgtaa gctctctgtc
    49801 gctgatcctc ctggtgctgt tcatggtgcg ctcctttagt tggctgtttc tggttatgtt
    49861 catggtcttt gtttacctgc gtgattgtct gccagcggct gaatgcacat agcgggcaat
    49921 tgccgctttg cggttaatcg atgccagtgt tctggtcagt tctgcgttgt gtttgtgtgc
    49981 ctgctcttcg tggtactgcc gcatgatttt tccggcaggc gtgagcggct ccggctctcc
    50041 gggcggacgg cgcggacgaa cggcgacaag ccagctgcgg ttggtgctgt ccggtcgtcc
    50101 gttgagtatg tcggtgatga tggccgtggt ggtgcgatcg ggactgacga ccgtgatttc
    50161 cagctcatcg cccggttgtg gccccgtggc aatgttgttc agggtcagta agggcgtgcg
    50221 ctggcgcatc atttcgtcaa aacgctgtcg ctgcatggcg caataaaagc gggtcattgt
    50281 ggcgcctccg tattcggtgc gggggtgata tcaatgcggc gggtgctgtc attccagtac
    50341 gcggtgcgtc cgtcagccag gcgcacgcgg gcaccgtgac ggacggaaac gacggcgctt
    50401 tcatgcagct gcacgcccag cgcatcaagc tcgcgggtga tttgtgcggt gatgtgtggc
    50461 tcagcggtca gtgcgacact tacgcgaacg ccgccagcgg gcgcactgat gccgccgttt
    50521 ttcacggttt ccgtgtcagg tggcgggttt ttcagctgcc tggtgagttg ttttcgctct
    50581 gtgggggtca gtgaccttaa ccattcaccc aaatcacctt ccggcagggt aagtcgctgc
    50641 tccctgcgtc cgtccggctg cctttctggc gggtcgttta tggtcagatt ctggtcaggg
    50701 ggacagttat tgccacgagt ccaaggtgcg gcaaagccgc actcaacgtc aaaaccctcg
    50761 tccgtgcggt cttcgttttc cggcttacgt ctgaccagtt tccaggtgtc cgggtgggtg
    50821 catatggtgt agcgctcccc gagtgacggg gcgcggacgc cgtaaatgcg ttgcgggaat
    50881 tcgccgtagt cgttggcctc ttcggcagtt tcgtatgcgg tgcggaccag gtacgtgttg
    50941 cggggaatga gcacaccacc ctggcgggtg atataggtgg caaaacatcc cacgtcggcg
    51001 gcggccagta tggcgtcaat gtcgtcatgg tccagacgct gcggggcagc cgggttacgg
    51061 gccatctgcc cggcaaggcg gcggagttcg cgccatacct ggcggggagg gataccgaag
    51121 aactggaact ggcggatgcg gtgaagggcc gcccagccga tggcgttctc cacggtttcg
    51181 gccatggttt tgcctgattc cttatcgacg taaggtttgc cggttttcgg gtcattcccc
    51241 atgaaggcgc tggcgtccag gttcttaccg atataggtcg cgatatagct cgccggtgtg
    51301 ccttttgccg ggtcgatttt ttcgcattta aaacgcgggg agatatcatc acccagctct
    51361 gcgcggtcct ccctgatggc ggcgttgcgc atgatttcag tgatacgggc ttcgttttcc
    51421 tggctggtga ataccagcat gtgccagtgc ggtgtaccgt cgtggtgggg ctccacggtg
    51481 cgcacgccgt accagcccag cccggccttg tcgatggtcc tgcggacggc ggcgaaaaag
    51541 cggtaaacca gataatcact ggcatcgcgt accgttgctc cgcagtattt cgggttcagg
    51601 ctgccgtcat cgttcacgct gtgaaaacgg gaaggggctg tgacggtgac gaaaaacgcc
    51661 acgtcgccgc gtgcttcggc gacctgctcc attccccgga cgcaggccat catctcgtta
    51721 cggcggttac gcggattaga gagacctgcc cagtagatat cctcaaggct gaaccgttct
    51781 ccttcttcgt tctcgatgtc ccaggccttc agaaaatcgc gggtgcgctg gcgtttttcg
    51841 cgccagtcca gtaacgcttc atggctgata tataccgacg tcttacgtga caccaggcct
    51901 gcggcgcgta acagctcttc acgccagatg tcccggaggt gtcttaactg actgttccac
    51961 catgtcgcac aggaaaggcg catgaccgca cccggcacct gttccgggtc gggggcggtg
    52021 cggcggtcgg gcctgatttc cagcgacggc cagcagggtg gcgtgatacc cagttttaac
    52081 gccatgtgtg ccagtggctg gtaggtcagt aaccaggtga acaggtcatc gtcacccctg
    52141 ccggtctttt cgatgacctc atcacatgcg cgctccagtg cggcggaaaa cgcggtggcg
    52201 gtcagggtgg caaggtgctg gatttcgtca cggctcagct gcggcagcat aagcagttca
    52261 tccagccgct cgcgcgcggc ggtggcctta aaggccgggg tggcaaagcg ttcattcaca
    52321 cgggcgatac gtttcaggcg ggcacgcagc ccgtgacgaa cgaagttatc cgccgcatat
    52381 gcgctgttat tcttaagccc ttcccggcgt gcgctctcct gtttgcggct caggcgggcc
    52441 aggtcacgga tgagcggacc ggcaagaaac ggcggcaggg cgcgcagctc tgccatggct
    52501 tcagatacca gcgtgggtgg attttcgcgt ttgtgtcgcg tcagtttttc cagtctgatc
    52561 cgacgctctt cgtcgtgatg aagatgcgaa ttaattcgct tcccttcgtc tgtggttgtc
    52621 aggaaggcga cggtttcttc atccagtctt gcatcaatgc gaatgtcttc gttgatgtag
    52681 ctctgaatgg cctggcgcaa tgcggttggt tgcgctctgc cgaatgtttc aggatcgata
    52741 accggtagcg gggcgttcca cgggtacgcc agttgtgcgt tatggtcgtt catgcgtcgc
    52801 actccccggc agtcgccgcc tgaaacgccg cgcggtacag cacgagaagg cgctggtatt
    52861 caccgataaa gtcttctgcg gtgtatatac ctttcacggc cacgctgtgg tttgtcattt
    52921 ccagcacaag cgtcatctct gacgacgacc agctgatggc ggtgctgtcg gttccgttga
    52981 tgtgaaataa accaaaacca tcaccatcgc ctttcacgtc cacgcggtaa cggccgtcaa
    53041 cggtgaagga gaaatccccg tatgtggtga cgcgtctggc ggcctgttta caggcgcgtc
    53101 ggtaatagtc gtgccagtcg ttcaggcggg aagccagccg gggatccact gcccacatcc
    53161 agttaaaaaa gtcctgaata tctgaaagtc ccttaacgct cttcatgaga acctccggta
    53221 acagacgtgc ggaagcctcc cgcgcacggg tgcgggatgg cttcaggatg ttgtttagtg
    53281 gtggtgttgc tggttgataa ggtcctgcag ttctgccagg tcatccgcca gatagctgaa
    53341 gacggcggca cagtaacggt ctgacagcgc gtgtgcgtgt tcatgcagca tgttgatgta
    53401 catgatttgc gcgacgcgtg atgcgcgcca gagtctgcgg ttgatttcgg tgcggatgtg
    53461 aagacgctca gcgcgggcgc gctgttgcct gcggtttgcc atggtgtggc ctctttgttg
    53521 gtaagttctg aaaactcacc atccagaggt tgcaattctg ggtggcgaga cgtacggggt
    53581 tgcaaccacc ggtcaacaaa gaacccggcc cgaccgaagt cggccccgta cgccccacca
    53641 taatttcggt gcgaaaaaga tgtgacgata cggtacgcac aaaaaaaccg ctggcgcggt
    53701 tgtgcgcttt gttgatcagc gggggttgca atcccggcac ccgttttatg aggtgcggga
    53761 aaactgtaac ccgaccaggc gaacgctggc aagcggtttt tttgtgtgtg catgttctgg
    53821 tttcttagtg gttcagaaaa aaatcaaaaa ccttgtcaat gcgttgcagc agctctttct
    53881 gtattgcttc cggcgtttcc ggttcgcctg gcgcctccaa cgtcgcgcag aaatcctcga
    53941 tttcatgacg gagcgtcagg cgaatggcga gaggtgtggt tctggcgtgc tccagctcat
    54001 ccagcagcgc cagcacagca gacggcgaga gcattgcgcg aaacgccagt aatttttgag
    54061 gcgttgccat tcgttgcagg gcaaatgcca gttcgcgtag cttctggtgg ttgatggtgc
    54121 tcatgctctg gcttccttca gtagctggtt aaacatgtga gtaagtggat tgctacaccc
    54181 gaacggcatc gggtttacgt ggtaagaagc ctggcctcct gttttgcgag cgcgaccacc
    54241 tgtgctgcgg tttgttctga tgactaagcc gccgcgccag agtcggcgta actcagcatt
    54301 aatggctgtg gtctgggtat tcagtgccgt ggcgatttca cttccgctgc accccggatt
    54361 ggctgcgatg tagtccagaa tggtcatcgt gagatctcct ttgcagttcc ggttattcag
    54421 tcgggttcag ggtgtaatca atggcgtttt caatacggcg tttaaggtcc cggtggatct
    54481 cacctggtgt tactggtgcg cccggaccgc tcagcgttgc cgtgaagtcg tcgatttcct
    54541 ccagtaacag attgcggagt acggcgatgt ggtggcgtgc gtctttaccg ggtttacgtg
    54601 cgttggtggt gtacttgctg atcatggccg cagctctccg tgggcttgtg tttctgattt
    54661 ccatgcccct ctgatggtca ttacttcgcg aacggtactg gcaatcgcgg ttatttgtgt
    54721 gccgtcagcc agatggatgt agagataatc agcgtctgac gggcacggcg tggtcgttgt
    54781 gatcaaataa ttttccacta tgaactcgcg gcctgcggca tcggtgaaat gcgtcagcag
    54841 ggttcgtctt gtggcggtaa atatgctgtt catcgtcctg cccctcacgc tgaaaactgg
    54901 ccggactgtg cccggagttc tgccagttcg cgctcgaggc gcaggttttt catatagagt
    54961 tcactgatgg tctgttttgc tgtcaccatt gccatgcttt gcatttgcag ggccatgtag
    55021 gtggcaaaat gcttgtcttt atctatttct gatattcttt catccagtac attggttatt
    55081 tcagttagcg ttttttgcgt aaattcaata tgcgccgggt cggtaaatag tttgtttatt
    55141 tctgtaggtg tattgatgct catagctgtt tctccgtttc tggattgagt gaagccctcc
    55201 gcagatgcgg atggttttaa tgttttctgt ccggttggtt atttatttac ggcgttcttc
    55261 gccagtaaat aaagattcga tatttttcag cgcttcgata attcgcataa tgccaatcgc
    55321 cataagcacg gaaataatca gaatcaccca tgaaagaaat acgctcatgc tgccacctct
    55381 gtgaaataag gctcaacgat ttcaccttca aacagttcgg ccacaataac ttttaattct
    55441 tccagtgctt cgcggtcatc gctgcccagt gcgcagttat acagtcccat aattgcgccc
    55501 cgcagtgagt tacgaagtcc ttcgtcacgg gttacacccc ggtttgcaag ttcgaagtga
    55561 taacgctgca acgcattatt aataagtatt ttgtaggctt cgatcatggc tgaatccttt
    55621 tatttatcag cggcgatgtt ccgggtgctg aaaactgcga ccgctgatta accggtctgc
    55681 cgttcttaat gcttcgtgca gggtgaaatc ctgcccgaac tgctcatctc cgcgagtcag
    55741 ggagtaacag gtatttccgg taatcgggtt gcgtgcgcct ctgtgtatga caattccggc
    55801 atcattaatc agccaggtgt gtttaccggt ttgtttaacc agatgcccgt caggtgtggc
    55861 gtgcgtttcc tgcaggctgt agtacgagtt gctgcgtgat gcgctggttg agaaacgggc
    55921 tgcgtggcgt tctgtgccag tgcggaagct gcgtctctgt gtcatatcgg aaaactccgt
    55981 ttaagttaaa tgaaaatcag tgccgggatg tttaaccacg cccggcgcgt ggtattctgg
    56041 tgtttccaca caaccagaaa aaaggtaact aacatgattg ataaaagcgt taaggacgct
    56101 atcactgaat acctggttag catttacaca actaatcttg ctgtggctcc tgttgataag
    56161 ttgaccgacc caagagactc agtgggagtg gtgtccgatc tgcttatttc gctcctcatt
    56221 agcgaacttc ccgatgataa aagggatgca ttgataaaaa ctgcgtcaga aattttgcgt
    56281 cagatagagg ggcaacaagc ctttgctccc tatgccgtga atataaggac acgctttcat
    56341 agtattaatc atcttgcgtg ctttattcgc gcctctaaag acaaaccaga tctctgactt
    56401 gttcaacaat atatttttcc accccttctt tgactggcag gttccggtca agggagggga
    56461 aaagaatcaa tggtttttgt aaaaatccca ttaacgtcga taaaaactca attttcacat
    56521 tgaagccttc tgattttcgt cgcattattt cgcgttgtac gattggccac atgtagtcca
    56581 ggttaatttc ctttagctcc gcaacataat taaggtatgc ctcgcacccc tgaatgctta
    56641 caggtctaaa agaaatatcc agataattat caggaataat cgtactggtt gaataaataa
    56701 ttccacccac aaccctatct ttgtgcttta ttgctctgac aaaaaatgtt ttgccttcta
    56761 aatccctttc tgttgccagc caggggtctc ctgttatcag gcaatcctca tcaatggcga
    56821 ttacttcgcc agccttactc ccgtgttcgc tgtatttctt atgcatacct tcttcttctg
    56881 gtgtaatcgt catatatcct ccttcgtgcc gggttattcc gtcgttttgc ttttgtaaat
    56941 agtggtttta ttaagtgcca tttctccgaa tatatccaca gcctgctcaa cgggcagatt
    57001 aatcattatt ccacgggttg cgtatagcat tattccgatg catccatttt ctctactttc
    57061 gcaaaagtgt ccgaactctg cttctggttt aagtatcagg tcatcaatgt tttcattcgc
    57121 ttttagcttt gaacaaaagt taattaatcc ttcaagctct ttgtgtaact cgctataggt
    57181 tgttgtgatt gttattttcg gtgagccttc cagatgcatc agtatcgtgg cgtccttgtg
    57241 ttcctctttc aggaggctta actgcttaag gacggttgcg tttttttctg atttattttt
    57301 gccgctcatc gttcatcacc gttcgtttac atcctggctc tgttccagct ttgactttct
    57361 gcctttgtaa acccgttttc cattgacggt ctggcaatct tccttatcag ccggggtaaa
    57421 ctttcttttc tggcggtcgt atgtccagta gcttttccag ttgtgccatt ctggtggctc
    57481 gcattccggg agttcgtcgg ccagcttgtc ccactctctg acgttaaacc accaaacacc
    57541 atccgaattc tcatcttcag ggtttgagcg ggttttatgc cctggcatct ggttacgctg
    57601 gagagctgcg cgtaatgctg tttttgttaa tccgatgtag gcggcccctt tttcaagtga
    57661 gcaaagcccg gctattgggc catccagccg cagggttgcc ccttttttat atgtgcgctg
    57721 tggcttttgt tcgtcgttgt tatctgttgc ttcttgttga ctggtatcgg cgttgatgta
    57781 cgccgctcca gttgtgtgat cggtgtcaga taactcttta tcggcaatga agtcccgcat
    57841 ttgttatcct ctcgcacagg tgcgcctatt gcaccttatg aaggtttgtt ttggtttgtt
    57901 taatgtcgaa attacgacac ttttgcgagg attttagtgg tatgaattcg acactgtcaa
    57961 taagtatcgg tgaaaaaatc ttgatgatcc ggaaaagtga gaggcttaca aggaagcaat
    58021 tctttgagct tacagggatt cctgttaata cgcagaggta ttacgaaacg ggaaggcaag
    58081 atggaatcgg aagtgagatc ttattaaaga tcacaaaaaa tttacgtttt aaaaaatatg
    58141 cactttggtt aacaactgat gaaacgtttg aagctggggg tcaaattgcc ccggctctct
    58201 ctcttgatgg atcttctcag tcggagggcg atctggcttc aatcgacgtt acccaaaaat
    58261 catcccgatc aggccgcaat gctggctgac gttacacatc gagcgggttt catcccgcag
    58321 ggagtgtttg gttaatttat ccgttggagg ggctaatcat gtcgattaag caactcaaag
    58381 acggacgtta taaggttgat gtaaggccac aagggacgga aggaaagcgg attcgtaaga
    58441 tttttaccct gaagtcaaag gcgcaggagt ttgaaaggta tgttttgcag aattttcatg
    58501 ataagccatg gcaggctaag ccagcagatc agcgtcggtt atccgaactg atagaggcct
    58561 ggtggttgct tgatggtcgt aaccaggatt acggggatac ctacagggtc agactggaaa
    58621 agataaaccg tgagatgggt gatcctcgtg ccagtcagat gacgcgaaaa tttatgctcg
    58681 ggtaccggtc cgacaaatta caggccgggt taatgccttc cagcattaac cgcgatttgt
    58741 gtgccttgtc tgcaatgttc agctcgctga ttgagggtga ggtgttccat aacgaaaacc
    58801 cggttcgggg aatacgtaag ctgaaagtca ggaacacaga gatggccttt ctttcagatg
    58861 aggagattga acgactgctt gaacgactcg atggagatgc gcggcgtgtt gccattctgt
    58921 gtctttccac gggggcaagg tggggtgaag cagctggttt acgtggggaa catattgtag
    58981 gtaaccgcgt gatgttcttt aacaccaaga acggaaaatc tcgcgccgtt cccgtttctg
    59041 atgcggtact tgcgttaatt aaaacccgca gaaccggatt gttgtaccag gctgattacc
    59101 tcagatttcg ggagatcctt caggaggtga agccggactt accgaaaggg caggccaccc
    59161 atgtcatgcg tcatacattc gctacacatt tcatgatgaa tggcggaaat atcgttacgc
    59221 tacaacgaat cctgggccat gccacgattc aacaaacgat gacgtatgcg catttttcgc
    59281 cggacttttt acaggatgca atcagcttta atccgctggc tgaaagtgtc cataaactgt
    59341 ccatcgatta gttgtaactt gtgcttattt gctccccttg ttttgattta ctcattgaaa
    59401 aataagcaaa tcatcaaggg gagtaagtag cgttaaacct cctaaaggcg cttttttgct
    59461 atctgcgatt tgcgaaattg cctgatgcgc ttcacttagc agacgactat ttccggcaat
    59521 tcctgtctcc tcacctactg tgtcaatgca gccaacagct taaccatcgc gggcgtcacc
    59581 tgctgtgttt cataaacaat atataaatct gcagggatgc gctgtttgag cggacggaaa
    59641 atgacacctg gccagttcat ttgtgcgtag ctgtccgcta tcaatgtgat accaataccc
    59701 atactgacca tggcgagtac cgtttgcggt tcattaactt cgcgaataac aaccggtgaa
    59761 aatcccacca gctggcaaac tcgctgcaaa aaatcccagt cagtgtacac gggcggcatt
    59821 gtaacaaaat actcgtcacg tagcgcttcc agcggaacgg tggaaaatga tgagagatga
    59881 tgctcttcag gcatcgccac cagaaacgcc gattcatgca accgtaagct ggtaaaacct
    59941 gtcggtggtt ctgtcgccat tcgccagatc ccggcatcga gttcacgtcg ttccagcagg
    60001 gccatttgca tcgcgggcat cttttcgcga aaaagaactt caacgttagg attttccctg
    60061 aggaatcgcc gcataaccgg gcgcatccgt ccccacatag ccgttcccac tacgccgagt
    60121 tcaatccgcc ctgcttctcc ccgacctatt tgttcaaccc gagccaatac attattagca
    60181 ttcaccagca atcgacgcga ttcttccatc aagattttgc ccgcgtgtgt cagtacgacg
    60241 ctgcgcgaat ggcgaataaa aagctgcgtg ccgagttgat tttccagctc tttaatatga
    60301 atactgagcg gaggctgaga catatttaaa cgcgctgctg cgcggccaaa atgcaactct
    60361 tccgctacgg caagaaaata acggagcaac ttaagatctg ttctgtatac gcgttccata
    60421 attaagtgct ccatataata aatacatcgt attaaattat catggcataa agtatttcac
    60481 caccagatat ccgacataca taaacacaat ggataatata cctggtaata catttggtac
    60541 aggaataggc aagcgcacga ttgtaaacaa agcaccaatg ccaaaaccaa cgccggaaga
    60601 aaataaaata tctttcacta attaaccttt atcataaaag cagctctgaa gagcagagct
    60661 gcgaatcctt ttaatgagcc accgctcgat gctttatctt ttcagggtca tgattatatt
    60721 taaacccaaa gaaaaatatc actgcgagaa aaagagcata tcctgcaaac accagccaga
    60781 tagtttgcca gtcttttacg ccatccaccg aaaagtaatc tactgccatg ccactcagaa
    60841 tcgagccaac ccatgcgccg acaccattta ccatggtcat aaagagcccc tgcgcgctgg
    60901 cacgaatgct ggaatcaact tcctgttcga caaataccga accagaaata ttgaagaaat
    60961 cgaatgcaca gccataaaca atcatcgaca gcagcagcaa aataaatccg gttgttgacg
    61021 gatcgccata ggcgaagaag ccaaagcgca gcgtccaggc caccatactc atcagcatga
    61081 cggctttaat gccaaatcgc tttaaaaaga atgggatagt cagtataaag cccacttctg
    61141 ccatctgtga aactgacagt aaaatggagg gatatttcac cacaaaactg tcagcaaact
    61201 ccgggttacg ggcgaaatca tgtaggaacg gattaccaaa aacgttggta atttgcagta
    61261 ccgcacccag catcatggca aagaggaaaa agatggccat gcgtggattt ttaaacagca
    61321 cgaaggcatc cagacccagc ttgctggcaa gcgatgtggt cgcttttttc tccgcaaccg
    61381 gaatcttcgg caaagtcagc gcataagccg acagcagcaa tgacgcaccg gacgcgatat
    61441 acagctgcag actactcaat tccagatgca gcaggcttac tgcccacatc gcgacaatga
    61501 accccaccgt accaaaaacg cgaatgggcg ggaaagcggt caccgggtca agccctgcct
    61561 gggcaagaca ggaataagag acgctgttcg ataacgcaat agtcggcata aacgccatcg
    61621 cattgactaa catcacccaa aacatcatat ccggatcagt tacggatgcc gcataaaaaa
    61681 gtacgcccgc acacaccagg tgacacagca tgtatgcacg ttctgcgcgc agccatttgt
    61741 ctgcgatgat ccccattata ccaggcataa taatcgcggc gatccctttg gaactgtaaa
    61801 ccatgccaac attagcgccg gtgaaatgaa gggtattaat catgtaagag ccgagggtaa
    61861 ccagccagct cccccagata aaatattgca aaaaggacat tacctttaag cgcatcgcga
    61921 tgctcataat cgtttccttg tcgcatcatt tggcctcacc cgcgaggccg tttcttattg
    61981 tttttctcag gcaattttgc gcagaaagcc acaaataagg ttgatgaagt tctgcttaga
    62041 gagctccgca gccgccagcg tttgcacatg tgacaatttc acatcgctta aaccttccgc
    62101 catattggta atcgcagaga ccgcaacgac tttaaggtcg caatggcgag ctgaaataac
    62161 ctcaggcacc acagacatac caacaacatc cccaccaata atttgcatca tgcgaatttc
    62221 cgccgcagtc tcgaaattcg gccccggata cgagacgaac acgccctccg tcagagggaa
    62281 accctcttct ttcgccactt tttgtaacag tgcgcggtat tccgcatcgt aggcatttgc
    62341 cagtgagaag aagcgctctc caaaacgatc atcgttaaga cccaccatcg gcgtaccggg
    62401 catggtgttg atatgatctt tcaatgcgac caggctgcct gcccccactt ccggacgcag
    62461 cgagcctgcc gcattggtgc agaacagtag ctcgcagccc agcagcttaa aggtacgaat
    62521 tgcgtcagtc ataatggtca ttccacgacc ttcgtagaaa tgtccgcgac ctttcataca
    62581 taccaccggt acgccctgga gatggcccag cactaactcc cccgcatgac catgtaccgt
    62641 acttaccggg aagcccggca gcttttcgta ggaaattgcg acagcgttct caatctgatc
    62701 ggccagcgcg ccgagcccgg aacctaaaat aaaggccact cgtggcgtga aatcaggttt
    62761 ataagtcttg ataatatcta tgcaaaacag cgggttatga gaaaattgaa cctgagacat
    62821 acatatcctt tttctgtagg gtggaatcta acgccttttt atagcgaggt tgtcccggct
    62881 cttaccaata cacttttctc acgcattcaa tagcgattcg atatcgatgg atttatatct
    62941 tttctctatt aatccacggt aaaaactgta ttggcgatat taattccttt gcattaacaa
    63001 tatgtcgttt ttagcaaacc actttattaa gcaatctact aataacgatc ggcataggga
    63061 attatgctat gaaaaaacat cttttaactc tgacactttc ctctatatta gcgatacctg
    63121 ttgtatctca tgcagaattt aaaggcggtt ttgcagatat cggcgtgcat tatctggact
    63181 ggaccagccg gactaccgaa aaatcgtcga ccaaatcaca caaagatgat tttggttatc
    63241 ttgaatttga aggtggcgct aactttagtt ggggagaaat gtacgggttc ttcgactggg
    63301 aaaacttcta taacggtcgc cataataaac caggtagcga gcagcgttat acctttaaaa
    63361 ataccaaccg tatttacctg ggtgacaccg gatttaatct ctacttgcat gcgtatggca
    63421 cctacggttc tgcgaatcgc gtgaatttcc acgacgatat gttcctgtac ggtatcggct
    63481 acaatttcac cggcagcggt tggtggttca aaccgttctt tgctaagcgt tatacagatc
    63541 aaacctatta cactggcgac aacggctatg tcgccggctg ggttgcaggt tacaacttta
    63601 tgctgggcag cgagaaattc actcttacca actggaacga gtacgagttt gaccgtgacg
    63661 ctacctatgc agcgggtaat ggcggtaaag aagggctgaa tggcgcagtt gcactgtggt
    63721 ggaatgcaac atcacacatt actacgggga ttcagtatcg atatgcggat gacaaactgg
    63781 gcgaagattt ttaccaggat gcgataatct actccatcaa atttaacttc tgattgatta
    63841 ctgccggggc tcccggcagt atgaattcac tctatatctg atacaacgga tccccttccc
    63901 gaccagccat tagcgcattg ccctggtctc gtaccacgtc ccataacgct tcagcggcgg
    63961 tggaaagtga acggttcttc ctgcgcacca gcatcaactg gcgttcaaca accggcgtaa
    64021 tgcgctttac caccagtggg ctaccttcag gcaatggtag cgccagtgcg ggaagaatac
    64081 tgatgccgat gcctgcggct accatcggga acagcgtcgc cggatgtcca atctcctgca
    64141 caatattcgc ctgaatacca ttgcgcgcca gcgctgcgtc aatcagcggt cggctgcctg
    64201 acgcgtaatc ctgcaacacc aatttcgctc cctgtaatgc ctgccagggt acataatctt
    64261 cgacggccaa agcgctatcg cgatggcaaa gcagaaagaa aggctcggaa agaatcgctt
    64321 cacattgcag atcgcccact gggcctggat cgatgacgat gccaaagtcg acatctccct
    64381 ggcgaatact ttccatcacc cactgctgcg gacgatcgtg caggacaaac tgaatatctg
    64441 gatagcggcg atggctttca gcaatgcatt gcggaataag atgcgcggaa atggtctggc
    64501 tggcagcgac ccgaactttt ccgctcagtt gttgccccat acgcccggta tcgcgcaacg
    64561 tgctgttcag ttcatccagc agtcgctcaa gacgcaaagc cagctgctgc cctgcatctg
    64621 taagcaccac ttcacgcgtg gttctgtcca gcaggcgaac accagtatga ttttccagtt
    64681 ccttcacact gtgactcact gccgactggc tcaggccgat acgctctcct gcacgactaa
    64741 aacttttctc ctgcgctact gtgacgaaaa cctttaattg ttttaaagaa taattcatct
    64801 gttttcttca tgaatggatg caataaatca attttatttc tcaaactgga agaagcacaa
    64861 tagaaccatc gatcatctgg agtctttatg aaactttttc gtatcctcga tcctttcacc
    64921 ttaaccctga tcacggtggt gttgctggcc tctttctttc cggccagagg cgatttcgtc
    64981 cccttctttg aaaatctgac caccgcagcg attgccctgc tgttctttat gcacggcgcg
    65041 aagttgtcgc gtgaggcgat tattgctggc ggtggtcact ggcgactgca tttgtgggta
    65101 atgtgcagca ccttcgtgct gtttccgatt ctgggtgtac tgtttgcctg gtggaaaccg
    65161 gtaaatgtcg acccgatgct ctactccggt tttctctact tgtgcattct cccggctacc
    65221 gtgcagtctg caatcgcctt cacgtcaatg gcgggcggta acgtcgcggc ggcggtttgt
    65281 tctgcgtcag catccagcct gctggggatt ttcctttcac cattgctggt tggtctggtg
    65341 atgaatgttc acggtgcagg gggcagcctt gagcaggtcg gtaaaattat gctgcaactg
    65401 ctgctgccgt ttgtgttggg gcatctttcc cggccgtgga ttggtgactg ggtgtcgcgc
    65461 aataaaaaat ggattgcgaa aactgaccag acgtccattc tgttggtggt ttatacagcg
    65521 ttcagcgaag ccgtcgttaa tggtatctgg cataaagttg gctggggatc attgctgttt
    65581 atcgtggtgg tcagctgcgt tcttctggct atcgtgattg tagttaacgt ctttatggca
    65641 cgccgactgg gcttcaataa ggcagatgaa attactatcg tcttttgtgg ttcgaaaaag
    65701 agtctggcaa atggcatccc gatggcaaac attctgttcc ccacatcggt gatcggtatg
    65761 atggtgctgc ccctgatgat tttccatcag atccaattga tggtctgtgc ggtgctggcg
    65821 cgtcgataca aacgccagac cgaacagtta caggcgcagc aggaaagcag caccgataaa
    65881 gcttaaagcg gacgcttcag gggctggacc agttgcgtca gcccctcggt tttaatcagc
    65941 agcgtgattt gcattagctc accgagcttc cctgccggga actcatcttt gcgggcaaac
    66001 cacagcagat actcctccgg caggtcgatt aagcgacgcc ctttgtattt gccaaacggc
    66061 attatcgtat tggctatttc aatcagctgc tctttttcca tctcagctac ccagcaaacg
    66121 cagcatttcc gcttcgtcga tgacttcaat gcccagttcc tgcgccttcg ccagtttaga
    66181 tcctgcagct tcacccgcta tcaccagatc ggttttcttc gacacgctgc ccgcgacttt
    66241 cgcgcccagt tcgaccagtc gagctttagc gtcatcacgt gacatctgac ttaagctgcc
    66301 cgtaagcacc acggttttac cagcaaacgg gctgtcaatc tcttccgcgt tgataacgat
    66361 cggcgcaggc cagtggacac cttccgccaa cagctcgctg ataacattgc ggttgctttc
    66421 ttcggcaaag aagttgtgaa cgtgggatgc aacgacaatg ccaacatcag gcaccttttg
    66481 cagctcttca atcgaagcgg cttccagcgc ttccagcgtg ccgaaatatg ccgccagacc
    66541 tgctgcggtg gcctcgccga cttcacggat gccaagtgca tagaggaagc gagcaaaggt
    66601 ggtttctttc gctttttcta gcgcgttaac cacgttttgt gccgattttg gccccatacg
    66661 ctccagtccg gtcagtttgc ctgcggtgag tttgaacaga tctgccggag tatggacata
    66721 ttctttttca accagctgat cgatgatttt gtcgcccatt ccgtcaacat ccatcgcacg
    66781 gcgggaaaca aagtgtttca gcgactcttt acgctgcgca ccgcaaatca ggccaccggt
    66841 acagcgggca accgcttcac cttccacacg ctcaacgtca gaaccacata ccggacaatg
    66901 cgttgggaat acaacctcac gggtatcttc cgggcgttca gaaagcacga cgttaaccac
    66961 ctgcgggatc acgtcgccag cgcggcgaat caccacttta tcgccaatgc gtaaaccaag
    67021 acgttcgatt tcatccgcat tgtgtaaggt tgcgttactc accagcacgc cagcaacatg
    67081 gacaggttcc agacgcgcaa caggcgtaat cgcgccagta cgcccaacct gaaactcgac
    67141 gtcacgcaca aaggtcatct gctcctgcgc cgggaattta aacgctaccg cccagcgcgg
    67201 ggcacgcgcg acaaagccaa gctgctcctg ctgttccagt gagttgacct taatcaccac
    67261 gccgtcgata tcaaagccca gcgtcgggcg gtcttcttcc actttgtgat agaacgccag
    67321 cacttcttcc gccgattcac aaagcgttac ccgatcgctg accggcaacc cccacttttt
    67381 aaattgcagt aaacggccaa gatgagtatc cggcagctcg ccaccttcca gaacaccaac
    67441 gccatagcag aaaaaagtga gcggtcgctt cgctgtaata cgcggatcaa gctgacgcag
    67501 tgaaccagct gccgcattac gtgggttagc aaaaactttc ccgcccgtgc gtcgcgcatc
    67561 ttcgttaatc ttttcgaacc ccgccagcgg caggaacact tcaccacgca cttccagacg
    67621 cgccgggata ttctctccgt gcagcttcag cggaatagcg cgaatagtac gcacattaga
    67681 cgtgatatct tccccggtgg tgccatcgcc acgggtcgcg gcactgacta aaacgccatt
    67741 ttcatacaga atactgacgg caaggccatc cagcttcagc tcacagcacc aggtgacttt
    67801 ctcgttgttt ttcagacggt cctgcacacg tttgttgaaa gcaagaaagc tttcttcatc
    67861 aaaaacgtta tccagtgaca gcattggtac ttcatggcgt atctggctga aagccgccag
    67921 cggcgcagcg cctacacgtt gggtaggcga atcaggcgta atcagttctg gatgtttggt
    67981 ttccagctcg cgcagttcgc gcatcagcct gtcgtattca gcgtcgggaa tttccggcgc
    68041 atccatcaca tgataaagat attcatgatg gcgaagcgtc gttcgcagtt ctgtcagttg
    68101 ttgttcgatt gattccatat cgcaccatca atgctaaaaa cccccgacaa gcgggggttc
    68161 gaagaggagt taatttgcct taagtgtatc aggcgttggc gtctttgact tcgcggatga
    68221 tgtcctggta ctcgcgcaat ttctgcggag tcatcatacg gcgctgatcg tcaagcacga
    68281 caccgcccac ttcatcggca atatgctgcg cagattgcag catcagcttg aagttctgca
    68341 gctcgtcacc gtaagacggt acctgcataa agatggtgac acccggagta gtgaaatcct
    68401 tcatttcagg atcaaaggtt cccggtttca ccatattcgc caggctgaat aacgccgggc
    68461 cgctgccatc cgggctaaga tgacgatggt aaatattcat atcgccaaaa atgaagcccg
    68521 cttgttgaat gctgttaaga agcagttcac cgtttagctc gctaccgtga tgcgccgcga
    68581 cgttcatgat aatcaccgct tctttgcgct tcggtttatc cgtaactgga gccggttccg
    68641 ctacaggctc aggctgtggt gccgctacgg gttctgcagg ctggaaagcc tgttgtgccg
    68701 gttgcggtgc tgaatgcaca ggctgcggcg ctggcgcgac ctgtggcgaa actggctgct
    68761 gcaacggctg ttcaggctgc ggctgatagg caggctgctg caccggctgc gctggacgcg
    68821 gagcatgttg cggcggtacc tgcgcttcag gcggctgctg gaccggttga cgcggctgcg
    68881 cagacgcata aggcggttgg tactggtgtt gcggcgacgg acgagcagcc tcatgctcct
    68941 gagcgttagc cggggcatga ttcacgcggt gaacacgaac ctcaccaacg ccctcatcat
    69001 cttcgacatc ctcgtcataa gaatcgtcgt cacgttttga cttcattcgt tttaatggcc
    69061 gatcgcggaa catagaagat cgttctttac ggctggtcca gaaaccatgt accagtaaag
    69121 cgattatggc gatcgcgcca acaatgatta atatcagacg caaatcctgc atcattatat
    69181 tctctgttgt tctaacacct tgccaccacg gcaaacattt actcactaag agtatttgcc
    69241 gattacctca agtgcaagtg cactattaac tttcacagca caaagataga tgaaatcgtg
    69301 ctttttgctg ttttttcgaa catatcctaa ctgtccattg cgcaattacc cggtaaaata
    69361 cgcagaattt tcctgggatt ggtcaaaagg agctcatcct gattatggtt tcatcattca
    69421 catctgcccc acgcagcggt ttttactatt ttgcgcaagg ctggaagctc gtctcgcaac
    69481 ctgggattcg gcgtttcgtt attttaccgc tgctggtcaa tattttgttg atggggggcg
    69541 cattctggtg gctctttaca cagctcgatg tctggatccc gactctcatg agttacgttc
    69601 cggactggct acaatggctg agttatctgt tgtggcctct ggctgtcatc tctgtgctgt
    69661 tagtgtttgg ctatttcttc tccacgattg ctaactggat tgccgctccg tttaacggtt
    69721 tattggctga acaactggaa gcacgattga ctggcgctac accgccagat accgggattt
    69781 tcggtatcat gaaagatgtg ccgcgaatca tgaaacgcga atggcaaaaa tttgcctggt
    69841 atctgccgcg cgcaattgta ttgctaattc tttacttaat ccctggtatt gggcaaaccg
    69901 tcgcgccggt actgtggttc ctgtttagcg cctggatgtt agccatccag tattgcgatt
    69961 accccttcga taaccacaaa gtgccgttta aagagatgcg caccgccctg cgcacacgca
    70021 aaatcaccaa tatgcagttt ggtgctttaa ccagcctgtt tacgatgatc ccgctgctta
    70081 atctgttcat catgcccgtt gccgtttgtg gcgcgacggc gatgtgggtc gattgctatc
    70141 gcgataaaca cgcgatgtgg cggtaacaat ctaccggtta ttttgtaaac cgtttgtgtg
    70201 aaacaggggt ggcttatgcc gccccttatt ccatcttgca tgtcattatt tcccttctgt
    70261 atatagatat gctaaatcct tacttccgca tattctctga gcgggtatgc tacctgttgt
    70321 atcccaattt catacagtta aggacaggcc atgagtaaga tttttgaaga taactcgctg
    70381 actatcggtc acacgccgct ggttcgcctg aatcgcatcg gtaacggacg cattctggcg
    70441 aaggtggaat ctcgtaaccc cagcttcagc gttaagtgcc gtatcggtgc caacatgatt
    70501 tgggatgccg aaaagcgcgg cgtgctgaaa ccaggcgttg aactggttga accaaccagc
    70561 ggtaataccg ggattgcact ggcctatgta gctgccgctc gcggttacaa actcaccctg
    70621 accatgccag aaaccatgag tattgaacgc cgcaagctgc tgaaagcgtt aggtgcaaac
    70681 ctggtgctga cggaaggtgc taaaggcatg aaaggcgcaa tccaaaaagc agaagaaatt
    70741 gtcgccagca atccagagaa atacctgctg ctgcaacaat tcagcaatcc ggcaaaccct
    70801 gaaattcacg aaaaaaccac cggtccggag atctgggaag ataccgacgg tcaggttgat
    70861 gtatttattg ctggcgttgg gactggcggt acgcttactg gcgtcagccg ctacattaaa
    70921 ggcaccaaag gcaagaccga tcttatctct gtcgccgttg agccaaccga ttctccagtt
    70981 atcgcccagg cactggctgg tgaagagatt aaacctggcc cgcataaaat tcagggtatt
    71041 ggcgctggtt ttatcccagc taacctcgat ctcaagctgg tcgataaagt cattggcatc
    71101 accaatgaag aagcgatttc taccgcgcgt cgtctgatgg aagaagaagg tattcttgca
    71161 ggtatctctt ctggagcagc tgttgctgcg gcgttgaaac tacaagaaga tgaaagcttt
    71221 accaacaaga atattgtggt tattctaccg tcatcgggtg agcgttattt aagcaccgca
    71281 ttgtttgccg atctcttcac tgagaaagaa ctgcaacagt aatgccagct tgttaaaaat
    71341 gcgtaaaaaa gcaccttttt aggtgctttt ttgtggcctg cttcaaactt tcgcccctcc
    71401 tggcattgat tcagcctgtc ggaactggta tttaaccaga ctaattattt tgatgcgcga
    71461 aattaatcgt tacaggaaaa gccaaagctg aatcgatttt atgatttggt tcaattcttc
    71521 ctttagcggc ataatgttta atgacgtacg aaacgtcagc ggtcaacacc cgccagcaat
    71581 ggactgtatt gcgctcttcg tgcgtcgcgt ctgttaaaaa ctggcgctaa caatacaggc
    71641 taaagtcgaa ccgccaggct agactttagt tccacaacac taaacctata agttggggaa
    71701 atacaatgtt ccagcaagaa gttaccatta ccgctccgaa cggtctgcac acccgccctg
    71761 ctgcccagtt tgtaaaagaa gctaagggct tcacttctga aattactgtg acttccaacg
    71821 gcaaaagcgc cagcgcgaaa agcctgttta aactgcagac tctgggcctg actcaaggta
    71881 ccgttgtgac tatctccgca gaaggcgaag acgagcagaa agcggttgaa catctggtta
    71941 aactgatggc ggaactcgag taatttcccg ggttctttta aaaatcagtc acaagtaagg
    72001 tagggttatg atttcaggca ttttagcatc cccgggtatc gctttcggta aagctctgct
    72061 tctgaaagaa gacgaaattg tcattgaccg gaaaaaaatt tctgccgacc aggttgatca
    72121 ggaagttgaa cgttttctga gcggtcgtgc caaggcatca gcccagctgg aaacgatcaa
    72181 aacgaaagct ggtgaaacgt tcggtgaaga aaaagaagcc atctttgaag ggcatattat
    72241 gctgctcgaa gatgaggagc tggagcagga aatcatagcc ctgattaaag ataagcacat
    72301 gacagctgac gcagctgctc atgaagttat cgaaggtcag gcttctgccc tggaagagct
    72361 ggatgatgaa tacctgaaag aacgtgcggc tgacgtacgt gatatcggta agcgcctgct
    72421 gcgcaacatc ctgggcctga agattatcga cctgagcgcc attcaggatg aagtcattct
    72481 ggttgccgct gacctgacgc cgtccgaaac cgcacagctg aacctgaaga aggtgctggg
    72541 tttcatcacc gacgcgggtg gccgtacttc ccacacctct atcatggcgc gttctctgga
    72601 actgcctgct atcgtgggta ccggtagcgt cacctctcag gtgaaaaatg acgactatct
    72661 gattctggat gccgtaaata atcaggttta cgtcaatcca accaacgaag ttattgataa
    72721 aatgcgcgct gttcaggagc aagtggcttc tgaaaaagca gagcttgcta aactgaaaga
    72781 tctgccagct attacgctgg acggtcacca ggtagaagtg tgcgctaaca ttggtacggt
    72841 tcgtgacgtt gaaggtgcag agcgtaacgg cgctgaaggc gttggtctgt atcgtactga
    72901 gttcctgttc atggaccgcg acgcgctgcc cactgaagaa gaacagtttg ctgcttacaa
    72961 agcagtggct gaagcgtgtg gctcgcaggc ggttatcgtt cgtaccatgg acatcggcgg
    73021 cgacaaagag ctgccataca tgaacttccc gaaagaagag aacccgttcc tcggctggcg
    73081 cgctatccgt atcgcgatgg atcgtaaaga gatcctgcgc gatcagctcc gcgctatcct
    73141 gcgtgcctcg gctttcggta aattgcgcat tatgttcccg atgatcatct ctgttgaaga
    73201 agtgcgtgca ctgcgcaaag agatcgaaat ctacaaacag gaactgcgcg acgaaggtaa
    73261 agcgtttgac gagtcaattg aaatcggcgt aatggtggaa acaccggctg ccgcaacaat
    73321 tgcacgtcat ttagccaaag aagttgattt ctttagtatc ggcaccaatg atttaacgca
    73381 gtacactctg gcagttgacc gtggtaatga tatgatttca cacctttacc aaccaatgtc
    73441 accgtccgtg ctgaacttga tcaagcaagt tattgatgct tctcatgctg aaggcaaatg
    73501 gactggcatg tgtggtgagc ttgctggcga tgaacgtgct acacttctgt tgctggggat
    73561 gggtctggac gaattctcta tgagcgccat ttctatcccg cgcattaaga agattatccg
    73621 taacacgaac ttcgaagatg cgaaggtgtt agcagagcag gctcttgctc aaccgacaac
    73681 ggacgagtta atgacgctgg ttaacaagtt cattgaagaa aaaacaatct gctaatccac
    73741 gagatgcggc ccaatttact gcttaggaga agatcatggg tttgttcgat aaactgaaat
    73801 ctctggtttc cgacgacaag aaggataccg gaactattga gatcattgct ccgctctctg
    73861 gcgagatcgt caatatcgaa gacgtgccgg atgtcgtttt tgcggaaaaa atcgttggtg
    73921 atggtattgc tatcaaacca acgggtaaca aaatggtcgc gcctgtagac ggtaccattg
    73981 gtaaaatctt tgaaaccaat cacgcgttct ctatcgaatc tgatagcggc gttgaactgt
    74041 tcgtccactt cggtatcgac accgttgaac tgaaaggcga aggcttcaag cgtattgctg
    74101 aagaaggtca gcgcgtgaaa gttggcgata ctgtcattga atttgatctg ccgctactgg
    74161 aagagaaagc caagtctacc ctgactccgg ttgttatctc caacatggac gaaatcaaag
    74221 aactgatcaa actgtccggt agcgtaaccg tgggtgaaac cccggttatc cgcatcaaga
    74281 agtaattctt gccgcagtga aaaatggcgc ccatcggcgc cattttttat gcttccgcca
    74341 gcggcggcaa aatcaattca tcgctctcat gctgctgggt gtagcgcatc acttccagta
    74401 cgcgcaaccc cgcacggtgc accgcatcgt ttaacgcctt ccctttcagc aaaccactga
    74461 taagctgagc acaaaacagg tcgccagtcc ctttcaggtc ggtttttacc cgtgaatggg
    74521 aaatgacatt cacgctgtcg gcactgacca ccaccacctg catctcctga ttttcttcat
    74581 taccggaggc gctggtaatc accacccatt ttaatgtgtc tgaaagcaga ctttttgcgg
    74641 cagcaatggc actgtcgaga tcgcggcaat ttttaccggt caggatttcc agctcaaaga
    74701 tattgggggt aattccctgc gccagcggca gtaaatattg tcgatacgcc tcgggaaggt
    74761 caggtttgac ataaattccg ctatcaatat cgccaatcac cggatcgacc atgatcaata
    74821 ggtcaggatg gtctttgcgt agcgcagtca gccactcggc aaggattttg atttgcgatg
    74881 ccgttcccat atagcccgtg gttacagcgc gcagctggcg cagcgcatca cgctcctgaa
    74941 gcgcacgcaa ataaccgcta aaccattcat ccggaatcgc accaccgtag aaagtgtcat
    75001 aatgcggcgt attgctcagc aataccgtcg gcacggcaaa aacattcaga ccgttctgtt
    75061 tgatagcagg cacggcaatg ctgttgccca cgctgccgta aaccacctgc gactgcacgg
    75121 cgacgatatc cgcctgcagc gccctgctct tatcgttaaa caacaacaaa ctactcattt
    75181 aattttttct ccttgccgat gatcctcatc gtattccaac cgaaacttta cctgattctg
    75241 gcagtcaaat cggctatcac aaaacaagga taaggtaatt caatgaagaa aatcatttgt
    75301 ctggtcatta cactactaat gacactcccc gcttacgcga agttaactgc ccatgaagaa
    75361 gcccgcatca acgccatgct ggagggatta gcacagaaaa aggatttgat atttgtgcgc
    75421 aacggtgatg aacatacctg cgatgaagcg gtttctcatc tgcgtctgaa gctgggcaat
    75481 acccgtaacc gcattgacac tgccgagcag tttattgata aggttgcttc gtcgtcatcg
    75541 attaccggga agccgtatat tgtgaagatc cccggtaaga gcgatgagaa cgcacagcct
    75601 tttttacatg cgctaattgc gcagacggat aaaacggtgc ctgcgcaata atcttaaatc
    75661 cccgccccct ggctaaaatg ctcttcccca aacaccccgg tggaaaggta gcgatcgcca
    75721 cgatcgcaga tgatcgctac caccaccgca ccagggttag cttttgccac ccgcagtgct
    75781 ccggcaaccg cgccgccgga gctgacgcca cagaatattc cttcccgcac tgccagttcg
    75841 cgcatggtgt tttctgcatc gcgctgatga atatccagca cctcatccac cagagaagcg
    75901 ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa tgccgggaat actgctgcct
    75961 tcttccggtt gcaggccaac aatggtcacc ggtttggatt gttcgcgcat aaagcgtgaa
    76021 acgccggtga tagtgccggt cgtccccata ctggagacaa aatgagtgat gcgcccgccg
    76081 gtttgctgcc agatttccgg cccggtggtg gtgtaatgcg cataagggtt atcgggatta
    76141 ttgaactgat cgagcagctt tccttcgcca cgattcgcca tctccagcgc cagatcgcgc
    76201 gcaccttcca tgccctgctc tttggtgaca agaatcagtt ccgcaccata agcacgcatc
    76261 gccgcacggc gttcctggct catgttgtcg ggcatcagca atttcatgcg atagccttta
    76321 agcgcggcaa tcattgccag cgcaatgccg gtgttaccac tggtggcttc gattaagaca
    76381 tcacccggtt taatttcccc gcgcttttcc gcctcgacga tcatcgaaag tgccgcacga
    76441 tctttcaccg aacctgccgg gttattgcct tccagtttta accacacttc actgccgtta
    76501 tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc ctattgtttg ttctaatgta
    76561 ctcacgatct ctatccatac gtggtgttgc ctgatgcgac gcttgcgcgt cttatcaggt
    76621 ctacaggtta caaaccttgc cattaaaaag cccggatcgc gggaagcgcc tccgggcgtt
    76681 taacattcac tcaacctatc aggcgctttg tgcgagagca agttcctcat cgcgggtttc
    76741 gatacgctcg tcgccgttat acagccgcgc atgttgcaga ccaacgaata aacgctcgcc
    76801 acgctgcggg gcatcgtcgc catgcatcac gaccgtcagc ggttcgttgt accaccccag
    76861 cggctgcacc actaattggg tgtagtgacc tttcgggctg gcttccagta cctgtaccgg
    76921 cagcggcgaa tcgagactgg tacggcggct gatatccact tcccacgggc gcaggaagag
    76981 atccaccggc ccctgataag caggtgtgta gcccagcggc cagcgatgtg cgccaacatg
    77041 gaactgcccg ccgcgaatgg ttccctgcag gcggttcact tcgcccataa attcgagtac
    77101 aaaacgggtc gctggttcgc gccatacctg attcggcgca tcagcctgtt caatattccc
    77161 ctgactcatc accactacac gatcggctac ttcggtcgcc tcttcctggt cgtgggtcac
    77221 aaacacgctg gtgaatttca gctcttcatg cagttgacgc agccagcgac gcagctcttt
    77281 acgcacctgc gcatccagcg cgccaaacgg ttcatcaagc agcagaattt gcggttctac
    77341 agccagcgcg cgcgccagcg ccacacgctg tttctggccg ccggaaagct gcgccggata
    77401 acgatccgcc agatgcgcaa gctggaccat ttccagcaat tttgtcactt tcgctttgat
    77461 ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc aggccaaaag cgatattgtc
    77521 gaataccgtc atatggcgga acagcgcgta atgctggaac acgaaaccga ccttgcgatc
    77581 gcgcgcgtgc aggcggctga cgtcggtgcc gtggaagcga atatgcccgc tggtttgatg
    77641 ctccagcccg gcgataatgc gcagcagcgt ggttttcccg gaaccggacg gccccagcaa
    77701 cgcgaccatc tgacctgaag gaatatccag tgagatatcg ttcagcacct gggtgcgacc
    77761 aaacgacttc ttaatattgg caatctcaat gctcatgatg ttcctcctgc tgtgcgcgtt
    77821 tttcctgatt ctccaggcgc cactgcaaca tactttttaa aaacagggtg ataatcgcca
    77881 tcagcgttaa cagtgccgca gcggtaaagg agccgacggt gttgtagtcc tgctccagca
    77941 attcaatctg taacggcagc gaaagtgttt cgccgcgaat cgagccggaa accaccgaca
    78001 ccgcgccaaa ctcgccaatc gcgcgggcgt tggtcagcac tacgccataa agcagcgccc
    78061 agcggatgtt cggcaatgtg acacgacgga acatctgcca gccagacgcg ccaagcaaaa
    78121 tcgccgcctc gtcttcctga ctgccctggc ttaacatcac cggcaccagt tcgcgcacca
    78181 caaacggaca cgtcacgaag atggtgacca gcaccattcc cggccaggag aacataattt
    78241 gcagattatg ttcatccagc caaccgccca gcgggccgtt agagccgtag aacagcagat
    78301 acaccagacc ggcaaccacc ggcgatacgg caaacggaat gtccagtagc gtcagcagta
    78361 actggcgtcc agggaagtta aagcgcgtca ccagccaggc cagcagaatg ccgaacacca
    78421 ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag ccagatggcg tgcagcatat
    78481 ccggatcggc cagattctgt aaaacaggca tcagcccttt gctgaacgcc tgtacgaaga
    78541 tataaatcat cggcaccagc aggatgaacg ccgataccag catcccgatg ccaatcagaa
    78601 accatttgcc ccagttaatc gggcgcgcgt cataacgctt caattgtgtc acttccgcca
    78661 ttaatgacct accacacgcc gaccaaagcg actttgcaga gtgttaattg agaacagcag
    78721 tatcagggat gccgcgagga tcaccgaagc aatcgcgctc gccgccgggt aatcaaactc
    78781 ctgcaagcgc acaaaaatca tcagcgacgt cacttccgtc ttccacgcga tatttccggc
    78841 gataaaaatc accgcgccaa attcaccaag actacgggta aacgacagcg ccacgcccgc
    78901 caccagcgcc ggagaaagct ccggcagcac cactttgcag aaactctgcc agcgcgttgc
    78961 accaagcgtt tccgccgctt cttcatattc cgggcctaac tcttccagca ccggctgcac
    79021 ggtacgcacc acaaacggaa tgctggtaaa ggccatcgcc accgcaatcc ccagccatgt
    79081 ataggtgact ttgatatcaa acttcgccag ccattcaccg taaaaaccgt ttacggaaaa
    79141 gagcgaggcc agcgttaaac cggcgacagc cgttggcagc gcaaagggta aatccatcag
    79201 cgcatcaagc agcgtgcggc ctgggaagcg atagcgggtt aggatccacg ccatcagcag
    79261 accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac agcagcgtta ctttgtaggc
    79321 cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc gcccagctca tctgggccag
    79381 ttgcatcacc agcgcggaga gcggcagcag caaaatcagg cacacaaaca gcagactggt
    79441 gccgaggctt aaggtaaagc ccggcagcac gcgtctggag gagacagcaa acatcagtta
    79501 cgccccgccg ctaacagctt gtctaactcg ccgccgctgg tgaagtgggt tttcatcact
    79561 tccggccagg agccaaattt gtcttccacg cggaacagct cggtctgcgg gaatttgtct
    79621 ttcagtttgt ccatcacctc cgggttattc acgcggtagt aatagtcggt gatgatggtt
    79681 tgcgcctgcg ggctatagag ccagttcaga taggctttgg cggctttttc cgtaccgttg
    79741 gcctgcacgt ttttatcaac ccacgccacc gggaattccg ccagaatgtt ggttttcgga
    79801 atcaccactt caaagccctg cgcttcatac tgtttacgga tgttgttcac ttccgattcg
    79861 aagctaatca gcacatcgcc caggccacgc tcggcaaaag tggtggttgc gccacgaccg
    79921 ccggtatcga acacttcaac gtttttcagg aactgggtca taaactgttc ggttttggct
    79981 ttatcgccac cgtcagcttt atccgctgcg ccccatgccg ccagataggt ataacgcgcg
    80041 ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt cggagcgcac caggtcgttc
    80101 caatcgtgga tattcttcgg gttaccctta cgcaccagga agcccatggt ggagtagaac
    80161 ggcgagctat tattcggcag gcgcgactgc cagtcggccg ggatcagctt gcctttatcg
    80221 tgcaggattt gtacgtcggt cacctggtta taagtgacaa cgtcggcttt taagccctgt
    80281 aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt gttttatcgt cagtttgtcg
    80341 ccgccgttat cttttgccca ttgttgctca aacggcggat tcagggcggc aaacagctcg
    80401 cgggagacgt cataagaact gttcagcagt tccgttgcct gtacatggcc cgccagcagc
    80461 agagaagcga ccagcgcgag tgagttcttt ttcagtaagt taacggccat tgcgcaccct
    80521 tataaattta atgactttct aatagccatc atatttataa cggacgtgaa aggagtaacg
    80581 gttttatata ccgtttggtg atttggaagt tgaaaaggga ataagaaccg ggtttaaaac
    80641 gaaatcctcc ccacaaatgc agggaggaaa cagaggtgaa tcagataccg acgctaaccg
    80701 tctccggcag tgtgctgccg ccatcaatca cattctgtgt accggttaaa tagctggatt
    80761 catccgatgc gaggaaggcc gccagttcgc cgacttccag cggatcggcg aggcgacgca
    80821 tcgggattgc tttcgccatt tcagtcagca ccgactctgg atcttccggg ttcgactggc
    80881 gggcaatgct ttccgccatt ggtgtgcgca cgtatcccgg gcaaatggcg ttaacgcgaa
    80941 taccagactg cgcgtactcc accgccagcg attttgtcag gccaacaatc gccgctttcg
    81001 ttaaggcgta cgccgtttcg ccaggatcgg ccaccatatc gccagtgacc gaagacatca
    81061 tcacaatgcg accatctttg cgggcaatca tctccggcag caccgccttc gtgacgttcc
    81121 atacgccttt aatattgatg tcaatatgga aatcgcgatc gtcatcgctc atatcgagga
    81181 aactgcccag acgacaaacg cctgcgttat tcaccaggat atcaatgcgc ccttcttttt
    81241 ccttcgcgcg tttgatagct gcggctaccg acgccgggtc acgcacatcg gcgacaaccg
    81301 ccgtacagcg atgaccacga ccacacagtt cgtccgccag cttttcgatc tcaggggaga
    81361 tatccagcaa gattaggttt gcgccatgac gcgcaaaagt tctggcaatt ccttcgccaa
    81421 ttccctgcaa tgcgcccgta atcagtgctg tcttgcccgt gagtttaccc attttaatgc
    81481 tctccttgtt aatacagagc gttacactcc attccctctg aaaatacagg ataaacaaaa
    81541 tattgctgcg gatcagctca ctaattcagg aaaatctcaa tccagtcgct gctttatgca
    81601 tcatttcagg cgttgtgtta gctcactgct gcgctgaatc attttcaatg actccacatc
    81661 attgagttgc acaagaccaa caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt
    81721 agacatcgac gagctacgcc attcggtttc accggaaacg gtatcgagcg tgaaatgcgc
    81781 cagccgccgc agcggagaat cagccagaga agtaatggca atcaccgttg ccccctgctt
    81841 tcgtgccgct tctgcgcata agacaatctc ttttttactg ccgctgtaag agatagcgat
    81901 ttgtacatcg ccttttttca acgcctggga aacggtggct tgtacgtgcg tgtcggcttc
    81961 gcaggccacg cgatagccaa ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc
    82021 cgatccgccc aggcccgtta tctggataaa cggtgcttta ctgatgacct caatgatttt
    82081 ttgcagccgc gcgtagtcga acaacgcaca ggtttgctcc agcgccagct ctttttcgcg
    82141 attcaatttg cgggcaatca cttccagcga atcatcactg gtaatcgaac tgtgcaggtg
    82201 cagcgccgtg gcattcgttt tctcccggct ggcactgtat tcgcctatta acgccatacg
    82261 caattcagta aatccttgcg cgccgagttt ttgggcaaat ttcactatgc tcgactggct
    82321 aatgccgagc tgtttcgcca tctgccgtga agaaacggat tgcagctcac tcacgttggc
    82381 ctgcagaaag tcagcgattt tttgctcgtt ttcggtgaat tcactccccg cattgcttat
    82441 tttcgtcagg tacaacatct tccactctcc tgggctggca gcataaattc cgcttaagtt
    82501 cttatgacgc tcttcacact ctgcgagtgt agactcaatg attcctttaa gtctgtcaat
    82561 tcgggatata tgattccata catctgcaat tatttgatcg taaatagtaa ggtcaccacc
    82621 gatgcaacta gaaaagatga ttactgaagg ctcgaacacc gcctcggctg aaattgaccg
    82681 cgtatcgacg ctggaaatgt gccggattat caacgatgaa gataaaaccg taccgcttgc
    82741 cgttgagcgc gtcctgccgg atatcgccgc ggcgatcgat gttatccacg cccaggtcag
    82801 cggcggcggt cgtctgattt atctcggtgc gggaacatcc ggtcgtctgg ggattctgga
    82861 tgccagcgaa tgtccgccca cctacggcgt gaaaccgggt ctggtggttg gtttgattgc
    82921 tggaggcgaa tatgccattc agcacgcggt ggaaggcgct gaagatagcc gggaaggtgg
    82981 tgttaacgat ctgaaaaata ttaatttaac ggcacaggat gtggtggttg gcattgctgc
    83041 cagcggtcgc acgccgtatg tgattgccgg actggaatac gcacgccagc tcggctgccg
    83101 cacagtggga atttcctgta atccggggag tgccgtttcc accaccgccg agtttgccat
    83161 tacaccgatt gtaggtgccg aagtggtcac tggttcttcg cgaatgaaag caggcacagc
    83221 gcagaaactg gtgctcaata tgctttccac cgggctgatg attaaatctg gcaaagtgtt
    83281 cggcaacctg atggtcgatg tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa
    83341 tatcgttaaa aacgccaccg gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg
    83401 cgagcgcaac tgtaaaacgg ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc
    83461 taaaaaacgc ctggatcaac acggcggctt tattcgtcag gttttagaca aggaataacc
    83521 catggccaaa gagatcagca gtgaacttct gaacaccatt cttacccgtg tcggcggacc
    83581 gggaaatatc gccagttgtg gtaactgtat gacgcgcctg cgtctgggtg tacatgacag
    83641 ttcactggtt gatcccaata tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag
    83701 cgaccaggtg caggtcgttt ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag
    83761 cgagctgctg ggcgaggcac cggtacagga tgccgcagaa atcgccgccc agaacaaacg
    83821 tcagttaaaa gccaaacaaa cctccggcgt gcaacaattt ctcgccaaat tcgccaccat
    83881 cttcacgccg ctgatccccg gttttattgc cgccggtctg ctgctgggga tagcgacgtt
    83941 aattgccacg gtgatgcacg ttccggcaga tgctcagggc acgctacccg acgcgctgaa
    84001 ttttatgaag gtgttcagca aaggtttgtt cactttcctg gtgattctgg tgggctataa
    84061 cgccgcccag gcatttggcg gcacgggcgt caatggcgca attattgccg cactcttttt
    84121 gctcggttat aaccccgctg caaccaccgg ttactacgcc ggttttcacg atttctttgg
    84181 tctgcccatc gatccgcgcg gcaatattat cggcgtgctg attgccgcgt gggcctgcgc
    84241 ccgcattgaa ggcatggtgc gccgctttat gccggacgat ctcgacatgc tgctgacctc
    84301 gttaatcacc ctgctgatca ccgctacgct tgcgtacctg atcattatgc cgctgggttg
    84361 ctggttattc gaaggtatgt catggctgtt tatgcacctg aacagtaatc cgttcggttg
    84421 tgcggtttta gccgggctgt tcctgatcgc cgtggtattt ggcgtgcatc agggctttat
    84481 tcctgtttac ctcgcgttaa tggacagcca gggattcaac agcttatttc ccatcctttc
    84541 aatggcaggc gcgggccagg tgggcgcggc gctggcactc tactggcggg cgcaaccgca
    84601 cagtgcatta cgcagtcagg tacgcggggc gattattccc ggtctgctgg gcgttggtga
    84661 accgttgatt tacggcgtca ccctgccccg catgaaaccg tttattaccg cctgtttagg
    84721 cggcgcagcg ggcggtttgt ttatcggcct gatagcctgg tggggtctgc cgatgggctt
    84781 aaacagcgcc tttgggccgt ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg
    84841 catccttccg gcaatggcgg tttatgctgg cgggattctg gtggcatggg tttgcgggtt
    84901 tattttcacc acgctctttg gctgccgtaa cgtcaatctg gactgatatc atgaaacgga
    84961 caatgctcta tctttctctg ctggctgtta gttgtagcgt cagcgccgca aaataccctg
    85021 ttctgacaga aagttcgcca gagaaagcag ggttcaacgt cgaacggctt aaccagatgg
    85081 atcgctggat tagccagcaa gtggacgccg gttatcccgg cgtaaacctg ctgatcatta
    85141 aagataatca gattgtgtat cgcaaagcct ggggagcggc gaaaaagtac gatggcagcg
    85201 tgttgatgga acagcctgtc aaagccacca ccgggacgct gtatgacctg gcctcaaaca
    85261 ccaaaatgta cgccacgaac ttcgccctgc aaaagctgat gagcgaaggc aaactgcatc
    85321 ctgacgatcg gattgcgaaa tatattccgg ggtttgccga tagtccaaat gacaccatta
    85381 aaggcaaaaa caccctgcgg atttctgacc tgctgcatca cagcggcggt ttcccggcag
    85441 atccgcaata cccgaataaa gcggtcgcgg gcgcgttata ttcccaggat aaaggccaga
    85501 cgctggagat gatcaagcgc acgccgctag agtatcagcc cggcagcaag catatttaca
    85561 gtgatgtcga ttatatgctg cttggattta tcgtcgagtc ggtcaccggt cagccgcttg
    85621 accgctatgt tgaagagtcg atttatcgcc cgctcggcct gacacatacg gtatttaacc
    85681 cgctgctgaa aggctttaaa ccgcaacaaa ttgccgccac ggaattgaac ggcaataccc
    85741 gcgatggcgt gatccatttc ccgaatatcc gcacctccac tctctggggt caggtgcacg
    85801 atgaaaaagc cttttattcg atgggcggcg tttccgggca cgcaggtttg ttttccaata
    85861 ccggcgatat tgcggtgtta atgcaaacga tgctgaacgg cgggggctat ggcgatgtgc
    85921 agctgttcag tgcggaaacg gtgaagatgt tcaccaccag ctctaaggaa gatgccactt
    85981 ttggcctcgg ctggcgcgtg aatggtaatg ccaccatgac gccgacgttt ggcacgctgg
    86041 caagcccgca aacctacggt cacactggct ggaccggaac ggtgaccgtt atcgatccgg
    86101 tgaatcatat ggcgattgtg atgttaagca acaagccaca ttcgccggtt gccgatccgc
    86161 aaaagaatcc caatatgttc gaaagcggtc agctgccgat tgcaacgtat ggttgggtag
    86221 ttgatcaggt gtatgcggcg ttaaagcaaa agtaataaaa aggccagtca gtaatgttac
    86281 tgcctggcct ttaatcaatg aatcagaaac gcttacagcg ccatcaactt gtccagcgac
    86341 ggtgcgaaat aatagccgcc ggttaccggt ttggtgaaac gcaacatcgc atcacgctta
    86401 ccatcggtat cgccaaacat gctcagcagt tgctgctcaa tgttatgcag acgcgcgcag
    86461 taggcgcaga agtacagacc gtgagtgcca ctggcagtgc cgtacggcag gctctggcga
    86521 acaatcttca gccctttgcc atcttctttc agatcaacgc gggtgaggtg agaggtttcc
    86581 ggacgttcgt cgccgtcgat ctcttcgttg gcctctttgg tgcgcccgat catcatctcc
    86641 tgatcgtgaa cgctcatccg gttgagctgc ttcaggttgt gttcccaacg ctggacaaac
    86701 acatagctgc cgcccgcatc cacgccgtct ttgataaccg ccacttcgcg acgcgtctct
    86761 tcacccgccg ggttttccgt accgtcaaca aagccgctca ggtcacgctc ttcaacccaa
    86821 cggaagccgt ggatctcttc tttcacttca atgcagtcac caaaggcttc catcgccgcc
    86881 tgggcgacag agaagtttac gtcgtgacgc agagaaagaa tgtggatcaa cacatcgaac
    86941 tgggtggtcg gcgcaaggcc tttaccgtag cccggaaaat ctttcagctc ttctgcccca
    87001 acgccgccgc tcagagcgcg ccaggtgttg ttaccaaagg caaccaccgc accaagatgc
    87061 gcgtccggga atttcgcttc aaaagttgcc agtttgtcgg caaatgtttt actggccgca
    87121 cgcagggcgt caacttcccc tttcacgttg gcttcgatcc aaatcgccgc gcggcaatgt
    87181 tctggcaaaa tgccactctg aacctgagac attgttcctc ctgaaaataa taatgccacg
    87241 caatccgtgg cattgttgag cgctatttta cgtgtttttt cgcggcggcg gtttgttcag
    87301 acgcaaatta acgccgccag ataattttac tgactttcca gtttttcagg gtatcgtccg
    87361 aaggcattaa cccttcagga ccactccatt ccccgctgaa ctggtagcta atatgttgac
    87421 tgccttcggc tttacattcg acggcacgat tatcatcacc atcagctttt tggcaattgc
    87481 caaatgcttt gctgtaaagg tcgctaaacg gtgtaccgat tttaacaccg gtgtcagcag
    87541 gaatatcgct atccagcacg tcaatgcgac tgatcgtgcc ttgatcgccg ttaatcacca
    87601 tcgccacgtt gtcgcccttc atcacttcaa aaaagcgcac cacgttgccg ttcgcggttt
    87661 tcattccgct gcgcaggcga taatcgccat caagcgcatc ggcaatggct tgttcttgca
    87721 gtggtgtgga cgccgttaat tcaccaacgc cctgctcgct cactttggtg gacgacccaa
    87781 accagttcca cgggttagcg gcagaccagt taactgacga catcgtggaa cagccggtca
    87841 gcatcaatgg catagcgcat aacattaaac gcagcgattt catgtcactt cctttggtta
    87901 ttcaataacg ttgcttggag tgcaaattca ccaaaaagtg ccgttattct tttacttcat
    87961 taaaacaagc gcgtaaacgt cgattggtca gcagccagat taacgccaca atatccgcca
    88021 ctaccagcgc cagaccgata ccgctaacgg attcaccgtt cagccacagc cacggttgcc
    88081 agcaaagtaa aaccacctgc gccaacagca acagaaaata gagtacacgc caggtgcgag
    88141 gaaacgtagc ccgccgaccg ctcaacaaaa acgccagcac cgccggaatg ccaggaatca
    88201 gccccagcca gaaattatcg tgatcgggat aaaacagatt tagcagcgca gtaccctgct
    88261 cacgcgacgc accggcaatg acaaacagca cccaggttcg cgcctgaagc aatagcacaa
    88321 gccagaagag caagggtaaa cgcaggcgtc cgtgcgcatc ataatggaca ggatggaact
    88381 cagtactctt catcttcaat caaacgctta cccagactca gcacgtcggc gtgttcatat
    88441 cccaggcgtt catacattcc gagcaccatg tcgttatctt ccggcacatt gatctgaatt
    88501 ttcgggcagc cacgagcaat cagctttttc tccagccgat taagcaacgc attggcaatc
    88561 ccacgcccac gaaactctgg atgcacgcca agataataag cagacccgcg atgcccgtca
    88621 taaccgccca tcaccgttcc gaccacttca ccgtttacct cagcgaccaa aaacaaactg
    88681 acgtcatggt tcatcttacg ctcgatgtcc atttccggat cgttccacgg acgcagcaag
    88741 tcgcaacgct cccaaagggt gatgacctct tcgaaatctt cctggcgaaa tacgcgtatc
    88801 tccatggtat tcgttacctt tttgcgggtt aaaaggctga ttatggcgtg aacggtcgaa
    88861 ttagccaata tctgacgaaa atcggttgaa aaagtggcat aatggggagt tgtcaactat
    88921 tgaaatgaaa agtaaaacaa ttctcaacat caaaccgtcg taacggatta cgcgatacga
    88981 tataacatct ggaactttat tattacaact caggccgtat gagcactttt aaaccactaa
    89041 aaacactcac ttcgcgccgc caggtgctga aagccggttt ggctgccctg acgttgtcag
    89101 gaatgtcgca agccatcgcc aaagacgaac ctttaaaaac cagcaacgga cacagcaagc
    89161 cgaaagccaa aaaatctggc ggcaaacgtg tcgttgttct cgatccaggc cacggcggaa
    89221 ttgataccgg agcgatcgga cgcaacggtt cgaaagaaaa acatgtggtg ctggcgattg
    89281 ctaaaaacgt ccgttccatt ttgcgtaatc atgggattga tgcgcgttta acgcgttctg
    89341 gcgatacgtt tatcccactt tacgatcgcg ttgaaatcgc ccataaacat ggcgcagatc
    89401 tgtttatgtc aattcatgcc gatggcttta ccaacccgaa agctgccggt gcttcggtat
    89461 ttgccctctc taaccgtggg gcaagtagcg caatggcgaa atacctgtct gaacgcgaaa
    89521 accgcgccga tgaagttgcc ggtaaaaagg cgactgacaa ggatcaccta ttgcagcaag
    89581 tgctgtttga tctggtgcaa accgatacta ttaaaaacag cctgacgctc ggctcgcata
    89641 ttctgaaaaa gattaagccg gtgcataaac tgcacagccg caacaccgaa caagccgcgt
    89701 ttgtggtgtt gaaatcgccg tcggttcctt cggtgctggt ggaaacctcg tttatcacca
    89761 acccggaaga agagcggctg ttaggcacgg cggcgtttcg tcagaaaatc gccacagcga
    89821 ttgctgaagg cgtgatcagt tatttccact ggttcgacaa ccagaaagca cattcgaaaa
    89881 agcgataagt tatgaaaccc gacgcacacc aggttaaaca gtttctgctc aaccttcagg
    89941 atacgatttg tcagcagctg accgccgtcg atggcgcaga atttgtcgaa gatagttggc
    90001 agcgcaaagc tggcggcggc gggcgtagtc gggtgttgcg taatggtggt gttttcgaac
    90061 aggcaggcgt caacttttct cacgtccacg gcgaggcgat gccagcttcc gccaccgctc
    90121 atcgcccgga acttgccggg cgcagtttcg aggcgatggg cgtttcactg gtagtgcatc
    90181 cgcataaccc gtatgttccc accagccacg cgaatgtgcg tttttttatt gccgaaaaac
    90241 cgggtgcaga gcccgtctgg tggtttggcg gcggcttcga tttaacccct ttctatggtt
    90301 ttgaagaaga cgccattcac tggcaccgca ccgcccgtga cctgtgcctg ccatttggtg
    90361 aagacgttta tccccgttac aaaaagtggt gcgacgatta cttctacctc aaacatcgca
    90421 acgaacagcg cggtattggc gggctgttct ttgatgatct gaacacgcca gatttcgacc
    90481 actgttttgc ctttatgcag gcggtaggca aaggctacac cgacgcttat ttaccaattg
    90541 tagagcgacg taaagcgatg gcctacggcg agcgcgagcg caattttcag ctctaccgtc
    90601 gcggtcgtta tgtcgagttc aatctggtct gggatcgcgg cactctgttt ggcctgcaaa
    90661 ctggcgggcg caccgagtcg atcctgatgt caatgccgcc actggtacgc tgggagtatg
    90721 attatcagcc aaaagatggc agcccagaag cggcgttaag tgagtttatt aaggtcaggg
    90781 attgggtgta actccctcac ccccattccc gcatccgctg atgcaacgtc aacgacggct
    90841 tctcggcaaa cagctgctgg taatccgtgg caaattgccc cagatgccag aatccccact
    90901 gcatggcggc gtcttttacc gttgtgcttt gcgaccacgg gcttatcagt tcgcggcgta
    90961 cggcgttcag gcgaatgcgt ttcagccacg cgtttgggcc aatgcctaaa atagcgtgaa
    91021 acgcgttttg tagcgtgcgg cgactgacat gcagttgatt acacaagtcc agcaccgtca
    91081 ccggctcaga catattttcc agcacatatt cacgggcgcg ggaaagtaat ctacggtaac
    91141 tctgatgact gatgctttcc gccgtcacca ttggttgcgc gtcttccagc atcgccccca
    91201 tcgccattag caaattatcc cccagcactt ttcgcactgc aggctgatgg agattttccg
    91261 ggttctcgca aaacgtcgcc agcgcctgtt ggacaaagcc ccacagcgcg gctttatgct
    91321 gctctttcac ttccagcgcc gactggctac gcagcatatg taatacccga tccgggttat
    91381 gcaaaaagtt agcctgccgg gtgatgacat cttcagaaag caccacgccc aggatcgtgt
    91441 aatcatccgg cgtgctcagt tcaaattcag tgccaccagg gcgggtggcg atttccgcgc
    91501 ttcccagaca ttgcgaaccg ataaatccct gctcaccgcg cgtcgccgga atgccaaacc
    91561 agaacgagtt cggccagacc aggcacgact gacgcagcgc cagaccggtg tattcacgaa
    91621 aaacctgaat atcatcgagt agaatttccg taaattcacc atgaaacttg cccggatgca
    91681 gctgatcgta aatctgctgc caggcggtaa tcgttaaagc atgttcatag acatccgttg
    91741 tctgtcgttg atgaacatta tccacttcga ccttcggcgt gagcttcagg ttttcgggta
    91801 agggttcatg ataaagatgg tgcaaattgg ctgtacgggt ctttttcatg atgttaatgc
    91861 cgggtgttgt aggacacccg gcacctccga caggttaatg gggcttgaga cgataacgac
    91921 tactgcgttt acgtaacgtc ccggcagaaa agagctgttc gagcgcattt ctggcttttt
    91981 ccagcggcca gccaaagtgg gcagccactt ctcctgccgt cattccctga cgtactgatg
    92041 ttaacagcgc caacaactcg tcagcagatt ccgcgacgat aactggcgcg tcgggttccc
    92101 ttacgggttg cttcggctgg cggttaaagc cagtaaccag ccactgggtg tcatcgtctg
    92161 gccgaccgat ctccttgcgg ctgatgacac gcccggtacg catcgcggca gcacaacctg
    92221 cgtccagcgc cgcacgacac gccgccagat cgccttccac caccagcgtt aagcgaccag
    92281 ggtcaagcac ttcgtgactg agcagacgaa cgttagctgc tttgagcatg gcatccgctg
    92341 catctatcgc ggcgaccatg ccgtccactt ccagcaatcc cagtgcattg atcatcggca
    92401 acctccgtta cgcacgctgg attggattac gcgcgatttc cagcactgca tcggtaaagg
    92461 cgttacaggc tgctttacac gcggcctggc taccggttaa aaatgctgcc gagtagttgg
    92521 tttcagacgg tggcgggaca taggtcacca gctgtacgtc ggcagatttc aacgctgcat
    92581 caatgccata ggtcgcttcc agcggcggtg ccaccagata cgccatcgga tcgccaagcg
    92641 tgatcccggc ggttgatgag agataagaac cggtacgcga aaccacatgc gccaggaagg
    92701 cggtgttttc tgcgtcattg gcccactgga atgctgcacc attttcaata tgtgcaacca
    92761 tcgcatccag accggcacgc acttccgccg ggtttggccc accaagcata atcagcactt
    92821 caccggcagt cggtgacggg ccgtgtgcag cgcccgcata cagcgagcga ccatatacca
    92881 cttccaccat cgcctgcttg gtcgcttcat cagcggcaat gtaggtgacg tcatcggaat
    92941 cagcagaaat cagcccgagg ctacgaatat gcggcggcaa ttttagttca cgcgcaaatt
    93001 cagcgttaac ggaggcaatc acccgcatgg cggttaccga cggtcgaatc aaatctaaag
    93061 ctggcatgat gcctccttat cgggtcatgt tgatgccgga tgctttctgc tccagcatac
    93121 gtttcgccaa atccacaatg acggctgcgg cttcaaccgg cggcgtgccg ccctggtgaa
    93181 tgttagagat acaggtgcga tcggcctcaa cggtggtcgc catacgcggc gagtaaacgg
    93241 cgtagcagga gaggctttct gactgcccta accccggacg ttcccccacc agcaggatca
    93301 ccactttcgc gccgaggatc tcaccgatct gatcttcaat cttcacgcga ccataacgaa
    93361 caaagaatgg cgtgccgact ttcagcccgg cctgtttcag gcccgccatc agcggcggca
    93421 ggatctcttc gtagttcacg gtgatggcat cagttgacag gccatcagaa ataacgacct
    93481 gtacgtccgg attagcaaca cactgtgctt tcagcgcttc aacagcttct gcacacaggc
    93541 ggcggcccat atccgggcga gtcaggtaga gatttttgtc gctaatttca gagcgtactt
    93601 ccagcaagcc ctgcgctttc acccactctt ccggcacttc tttcagtacg gtatctttcg
    93661 aacgagagtg atcggccagg aaacgcagca gcgcctgggt acgtggacgc ggaccggcac
    93721 gaccggtaca aacgcgggcc acggtgctgc ggcgcagttc tgttaatacg tctgcgcgat
    93781 gcgggttttc aacaccaatc cacgcttttg cttcagcgga acctaaatcc agcgcacagc
    93841 tttccgaggt caccggtgcc gcacagttgg tggtggcgca ctttgcttct gacggcgcgg
    93901 gggccgtttg tcccattgac gccatcacgc tgcgtacaat ttcttcaatc tgtttttgat
    93961 ccatgatgtg ttatctccgc gtcatcagaa gaacagtgac ggatcgcccg cccgtttggt
    94021 caggcgaccg tttgccataa tgcccatgct ttccagccag cgttcaaact ccggtgacgg
    94081 acgcaggttg agtaactgac gcacagtggc ggtatcgtgg aatgcggtgg tctggtagtt
    94141 gagcatgatg tcatcaccca gcggcatccc catgatgtag ttgcagcctg cggtggcgag
    94201 caggatcatc aggttttcgt tgaggttctg gtcagcgtca gcgtggttgg tgtaacagca
    94261 gtcacagccc atagagatgc cgctcagctt gcccataaag tgatcttcta agccagcacg
    94321 gataatctgg cggtcgttgt agagatactc cggcccaata aagccgacca cggtgttgac
    94381 gataaacgga tcgtaatgac gcgccagccc atagttacgt gcttccatcg tcacctggtc
    94441 agcgccgaag ttagcgccag cggatagcgc agagccttgt ccggtttcga agtagaggca
    94501 gttttccccg gcgatacggt tgaactctgc acccactgcg cgcgcttcgt cgagcatcgc
    94561 cagttccacg ccaaactctt tcagcccttt ttcgctgcca cagatactct ggaaaatcag
    94621 tccgccaggc gcgccgcgac gaatcgcttc gatctgggtg gtgacgtgcg ccagtacgca
    94681 gccctgagtt gggatgttga atttgtcgat cacgccgtaa atggtgtcca acacgcggct
    94741 taagttttcc acgtcgtcag tcaccgggtt aacgccgatc accgcatcgc ccaccccgaa
    94801 ggaaagccct tcgtagattt gcgcggcgat actttgcacg tcgtcacggg tgtcgtttgg
    94861 ctgcaagcgg gcgctgaagg tgcccggaat accgatggtg gtattggcct ttttgattac
    94921 cggcattttc ttcgcgccgt agatcaggtc cgcgttggag caaatcttcg ctaccgccgc
    94981 gaccacttcc gaggtcagcc ctttgcgggt aaaggcaatg tcgtccacgc tggtttcatc
    95041 gctcagcaca tactcacgca gttcgctgat gctccagttt ttaatctggt tgtaggccgt
    95101 ttcgttaaca tcgtcctgaa tcagccgcgt cacgcagtca tcttcatagg caatcaccgg
    95161 attattgcgg atgtccgcga cggtcatttc cgacaacacc tgctttgcag ccacgcgctc
    95221 ctgtgaactt gccgctgcaa cgcctgccag cacatccccc gaacgcagtt cgttggcttt
    95281 agccagcacc tcttttacat ccttaaactg atatacattg ccgaacaatg tggtctttag
    95341 tttcataagt cgttccctca ggaaggaaat gcgagtgatt tcaccgtcac cggcacaacc
    95401 gatccgccaa aaagaggcgt accaatgtcg atatagtccc ccgcgcggac aatcacttcg
    95461 tcaatgactg ccaacgggag ttgttgtagc tgtgggcgca acaacatgcc cagagcttta
    95521 ccaaagtcct gcccggccac caccagcagg ggatgcggat tcggaaaacg cgcgacgaaa
    95581 tcgaccagcg cgttgatgac cgtcagtacc gcggcgtaac gcacaggcag cgaggcggga
    95641 agcgccagca cgtacgcgtc agttttggga tcaagatcca gctgaagcag cgcctgttgc
    95701 caggcactca ccagatccgt ttcatcaatc gggatcgcca ccggcaaatt gcgcagcggc
    95761 agttgtacgc cctcaagcca gattgtgctg ccagagagcg aaagggtatg tgcccccgcg
    95821 ccaatcaccg tggcacgcac ggtttgcgcc ggaaactgca cgttcatctc acgcaggcgc
    95881 ggatggtcat gcagcgccgt tgccagaagc gggccaatat cggcaaaaca gaacgggtcg
    95941 gcgggctggt ggcgataaca ttcgcccacg ccgccagaaa gcgtaatgat ttcgggcgta
    96001 acacctgcgg gcagcaaacc ggtttgcatc aatgcctgcg cgagcggcga aagcgttccg
    96061 tcaatcactt cgacaatcag ctctgccatc cgccgggtaa cctgcaccag ctgcgcgccg
    96121 gtcagcgaac gggcgtcagt gcctgcaccg aagcattcat ccacaatcat ctgccccggt
    96181 ttatgagcgt aaaccacgcg cccctggctg tcggtttcca gcaggcgacc accgacgttg
    96241 aggcaggcgg tgccgctgat ttttccggca tcgaacaggg cgtagttcgc ggtgccaccg
    96301 ccgatgtcga tattcagtac ccgacacagc cgttgttcag aaagggtttg cgccccagct
    96361 ccgtgaccgg cgatcacgga ttcgaggtgc ggcccggcgc tggcaacgac aaaatccccc
    96421 agcgattgag agagcgccat caccgccggg cgagcattgc gggttttcgc gctttcaccg
    96481 gtgatgatga tggcaccaga atcaacgctt tccggcgcaa tgcccgcagc ctgatattgc
    96541 tctaaaatca gcgacttcag ttccgcttct tctaaaccac cctgtttatc gacaggggta
    96601 aagaacaccg ggctttgcca gctaatttcg cgtttgatga attcgtagcg cggcacctgc
    96661 gacaccgccg cacggttaac cagttccagg tgggagaaaa tcacctgggt ggtggtggtg
    96721 ccgatatcga taccgacgct cagtagctgg cgagtgttca cgattgtgcc tccgcttcgg
    96781 ttttagctgc ggtcgcgtcg tcttttggca ccagcatcat cgccacgcca atcgccgtta
    96841 cgccgccgat caacttgccg acaatcatcg ggaagatcat ggcgttcatg ttggcagcgg
    96901 cgaagcctaa gtggtcgccc agggcgaaag cagcggaaac ggcgaaggcg cagttgatga
    96961 ctttgccgcg ggtatccatc tgcttcatca tgccaaacat cgggatgttg ttggcaagcg
    97021 ttgccaccat gccggctgcc gcgatgttgt tcatattcag cactttaccg acgctcatca
    97081 gcggtttttc aaaccagcga gtcagcagca gcaccatcgg atacgcccct aacagaacgc
    97141 aggagataga accgataact tcaatggcgc gcatcacctc accgggttta tcgccagggg
    97201 ccataaagat agggtccaga ccggggatca gttcccagcc aaggaggaat ttcactaccg
    97261 cagcggcaag accgagggtg atcaatgcaa cgaggaattt ggcaaagatc tggaagccgt
    97321 tgatcatttt ttccgggatg aatttcagcc ccagcgccac cagaatcgcg acaatgatca
    97381 ccgggatcat gttcatcagg atcagggcga aagtgaattc caccggctga ccgttgatct
    97441 gcacaccgga gtacatagca accagaccac cagcaataca gccaatcgga atggtcacaa
    97501 tgcccgccag cacgccgagc gccagataac gacggtcaga aggttcgata atgccgagcg
    97561 ccaccggaat ggaaaacaca atcgttggcc ccatcatcga cccgagaatt aacccagagt
    97621 atagccacgc tgctacgtcg ccgcccgcca gctctttggc gaggaagaag ccgcccatat
    97681 cgcacgccag cagcgttccg gcgaacattg acgggtttgc gccgagcatt tcgtaaaccg
    97741 ggataattac cggcccgagt acgtgagcca gtaccggtgc cagcgcggtc ataccgacca
    97801 tcgccaggcc cagtgcgccc attgccataa agccttcttc aaactgaccg ccggatcctt
    97861 cgatactttt accgaactta ccgaggaaac gagcagaacc gccgaactgc gacaggatcc
    97921 tgtctacggc agctatcagc ataaagaaca tcatgatgta catgatgatt tcgttaattc
    97981 ccatcgcctt tactccctgt tagttgttat ttattggcgg atgcggcgta aacgccttat
    98041 ccgccctaca tgtgcaatcc cgaaggccgg atcagacgcg gcaagcgtcg catccggcat
    98101 ttattgcgcc gctgcgtaca agccgacaat ctgctccagg ctggcggtac gcgggttgct
    98161 gcgcagacaa atatcttcct gcgcggcctg cgcccatgca ccgtaatgcg cagatgtcgc
    98221 gccaacatcg cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt
    98281 aatagcgtca tgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg
    98341 ttcacgacaa accatccggt taaattccat cactgttggc agcaacatgg cgttcgccag
    98401 accgtgcgga atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag
    98461 acccgcactg gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc
    98521 ggcaaggtcg tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc
    98581 accaatcgcc agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc
    98641 atgggttaac gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa
    98701 tgcggcgtcg aggatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg
    98761 cccgctcacc gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt
    98821 agttggaatg gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc
    98881 cagcgtgcta tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga
    98941 gccgccgcca aatgcgatca ccccgtcaca acctgactca cgcaactgcg ccacggctgc
    99001 acacacgtcg gtaatgcacg gttcgcccac cggacatagc cagagcgtca tggcgatacc
    99061 tttaacggcc aggctacgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc
    99121 tgccatcacg aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac
    99181 cgagcccgga ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt
    99241 cagggtatcg aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg
    99301 ctttctctcg cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta
    99361 aatgcacttg taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac
    99421 aggagccgcc cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa
    99481 tgtccgccat tttttcgtac accggtttca ccgcaggcca aatctcttca ccgtgaccgc
    99541 gcttgtactg ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga
    99601 tgtgatgtcc gccggtggct tcatccgccg agtacgtcac tttgcccttt ttcacgatgg
    99661 caatgccggt agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat
    99721 ccgcaactgc ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca
    99781 cgttgatgga aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac
    99841 ggcgaccgaa ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga
    99901 agaaatccca gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg
    99961 gctgaccgtc gcggtcaaca accatcgaca ccacatcgca ggtgcccaga tccaccccca
   100021 gccacagcgg agattccgtc gcggcaggcg tctggttaca cagcgttgcc gccgtttgca
   100081 gacgtggggt gagccattgt tcgtcgtgcg ccatcggtta ctccttaaac aatgcgaaac
   100141 gcatcgacta atacacagcg acgcagacgg acaaacgtcc gcgcgctggt taccccttca
   100201 ccggttggcg tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca
   100261 atgcacggtc cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg
   100321 ttttcgatgt tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc
   100381 gccagcgcaa tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc
   100441 agttcggtca cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct
   100501 tgcggcacgt tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca
   100561 cggctgacgg tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc
   100621 tgcgcctgtt ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca
   100681 tcggctacgc tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg
   100741 aaagaagcgc ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc
   100801 actaccggcg ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc
   100861 gcttctacta ccgcttcgcc gccggtcacc accagcaggc cgatacccgg aaacttgaac
   100921 aagcgttgcg cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggccca
   100981 cctgcggcaa caatcgcctg gttgagcagc gtaatcgccc gctgggagac ttttttcgcc
   101041 gccggatgcg gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta
   101101 attacggttg ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg
   101161 ttttcaatta aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg
   101221 cctggcgtgc cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg
   101281 ccggtttcac tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga
   101341 atggcagcaa tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg
   101401 gctgccgcaa cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcggac
   101461 ggcgtgtcac tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt
   101521 tgattcatga tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat
   101581 cgacaatgcc aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg
   101641 cggaactgcc actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg
   101701 cgacggcgca ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt
   101761 tgtcatgcgc cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca
   101821 gtttcatacc cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag
   101881 gtaaaatgaa aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg
   101941 ttgctgctat cgcctttcag gccgatcggg aagacttctt ccaggtcacc gtgcggacgc
   102001 gggataacgt gtacggatac cagctcgcca atacgctgtg ccgctgcggc accggcatcg
   102061 gtcgccgctt tacaggctgc aacatcaccg cgaaccatgg cagtacacag gccgccgcca
   102121 atctgcttaa caccaaccag cttgacgcgc gcagctttca ccatcgcgtc agaagcctca
   102181 atcagtgcaa ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct
   102241 ttatcagggt ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt
   102301 tcacttttgt ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc
   102361 tacaaccacg agagagatcg tgcatcggcg cggcaagtcc ttgtatcagt ggcccgacgg
   102421 cacgatatcc gccgagtcgt tgtgcgattt tgtaaccaat atttccggct tccagcgacg
   102481 gaaaaaccat cacattggcc ttgccctgta gcgggctggc aggcgctttt tgcgccgcca
   102541 cttccggcac gaaggcggcg tcaaactgta actcgccatc aaccaccagc tttggtgcgc
   102601 gctcacggac gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac
   102661 cgttactgga aaacgatagc atcgccacgc gcggctcttc tccggtgatg gcgcgccagg
   102721 tttcggcact ggcaagcgcg atatccgcca gctgcgccgc cgtcggctgt ggcaccacgc
   102781 tgcaatcggc aaagcccaac gccgggccgc tgtactgtgg caacatcagg aaaatggaag
   102841 agagcgtttt acagcctggc tgcaagccga taatgcgtaa tccggcacgc agcacatttg
   102901 ccgtggaaga gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg
   102961 cggcgaacat cagagggtcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg
   103021 cgcgggccag ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa
   103081 tcacctgtag cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg
   103141 gattggcgac cagaatgggc gttgccagac cttgttgatg taaatattgc gcggctttca
   103201 gcacacgttg gtctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt
   103261 cacgacaacg ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt
   103321 tagcggctgc ttcgttacgc tcaggatcat catcacgtag accgtgctcg aaaggcggtt
   103381 cagcgcctgc aaaatgtccg ggcgtaatac ttcaaagctg cgggtgataa agacctgcgc
   103441 cgctacggtt tcggtttccc gcactttggt gcgtagcaga ttgagcaacg cagcatcgcg
   103501 cccgtggctg gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctgatgaga
   103561 aagccgatgc agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc
   103621 cagcgcatcg gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg
   103681 ccacggttcc gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac
   103741 cgcgcgaaaa cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt
   103801 cagcgtatcc ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg
   103861 ttcatcgcta ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa
   103921 caggcggccc tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg
   103981 ggcagagggc gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt
   104041 atggttcgct cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc
   104101 ggccaggcga catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct
   104161 ttcgggataa acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca
   104221 tgtagctccc cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat
   104281 gcgttctccc actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga
   104341 tcagtcaagc ccacgcaatg cggctctgcg ccgtcgaaac gaccaaactt gacgctgctg
   104401 ccatcaatca ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg
   104461 ccctgctcca gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt
   104521 tcggtaaact ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg
   104581 gttttgtcgg ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat
   104641 tcggtgatgg taaaacccag tagatccgcc acttcacggg cttccggggt aataatgctg
   104701 gcgcgcagaa ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc
   104761 gctgtgatga gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac
   104821 cagttgctgc acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc
   104881 cgtctcaagc aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact
   104941 gatgacggcg atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact
   105001 ttccgggtca tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat
   105061 taaggcgtga taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt
   105121 atcattaaat tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt
   105181 aaataacgtt gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc
   105241 ctcgtattaa cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct
   105301 gacggtttgt gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc
   105361 gctgaagcgg tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccggc
   105421 gatcatcgcc gtttcgccgg gagttagcgt cataatgcca attgcgcccg catccggaac
   105481 gccgattttc ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg
   105541 tttgccgggc acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa
   105601 aactttcgtg tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt
   105661 ttaaaaggtg aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc
   105721 atatgcaacg catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt
   105781 attattcgca aactcagtaa taagctttac taatatattg cagccaaacc gcttaccctg
   105841 aatattcagg gtaagcgtga gagttaaaaa agaattacag cggttgggtt tgcgcttcaa
   105901 ccacggccag cgccaccatg ttgacgatac gacgcaccga tgcgatcggc gttaacacgt
   105961 gaaccggttt cgccacgccc atcagcaccg gaccgacagt cacaccttcc gagctggaaa
   106021 cgcgcagtaa gttgtaacta atgcgggcag cttccatgtt cggcatcacc agaatattgg
   106081 cagaaccttt caaagggctg tccggcatac ggtcgttgcg aatcgcttcc accagcgctg
   106141 catcgccgtg catttcacca tcaatcatca gatctggcgc acgatccctg accagttcca
   106201 gcgcctgacg cattttgctc gacgacgggc agtcagaaga accaaagttg gagtgcgaca
   106261 acagagcaac gcgcggctca ataccaaaac gacggacagt ttctgccgcc atcaaggtga
   106321 tctccgccag ctcttctgcg tctggttcgt cattgacgta ggtatcggca ataaaggtgt
   106381 taccactcgg cagcagcagc gcgttcattg cacctgcggt gtgaacgcca tcgcgataac
   106441 caaagacatt tttcaccacg ctaaaatgtt catgataatc gcccaccgta ccgcagatca
   106501 ttgcatcggc ttccccacgc tgaaccatga tcgcgccgat cactgtcggg ttactgatca
   106561 gcgcccgctg cgcctgttcc tgagtgacac cgcgacgctt catgatctgg aagtattcgg
   106621 tccagtactc tttaaagcgc ggatcggatt cgttattgac gatctcaaaa tcaacgcccg
   106681 ctttgatctg caagcccagt ttctgaatgc gcatttcgat cacgttcgga cgaccgataa
   106741 ggatcggttt cgccaatccc agcgttacca gttcctgagt ggcatgcaga acgcgcgcct
   106801 cttccccttc tggcagaaca acgcgcttcg gcgctttgcg agcctgggag aaaatcggct
   106861 tcataaacag gttggttttg taaacgaact cagtcagctt gtcgatgtag acgtcgaaat
   106921 cagcaatcgg acgcgtcgcc acgcccgact ccatcgcggc tttagcgacc gcaggagcga
   106981 tcttaacgat caagcgcgga tcaaacggtt ttggaatgat gtattccgga ccaaagctca
   107041 gatcctgatc gccatacgct gaagccacca cttcgctctg ttcggcgtgg gccagttccg
   107101 caatcgcgcg taccgccgcc agtttcatct cttcattgat agccgttgcg ccaacatcca
   107161 gcgcgccacg gaagataaac gggaagcaaa ggacgttgtt gacctggttt ggatagtcag
   107221 aacgaccggt gcaaatgatg gcatccggac gcacttcttt cgccagcggc ggcagaattt
   107281 ccggttccgg gttcgccagc gccaggatca ttggcgcacg agccattttc ttcaccattt
   107341 cctgggtcag cactttcggg ccggaacatc caaggaaaat atccgcgcct tcaatcacat
   107401 catcgagggt acgtttgccg tcatccacca ccgcatatgc ggctttggtt tccgccatgt
   107461 ttggctcacg gccctgatag ataacgcctt ttgaatcgca aaccacgatg ttatgttttt
   107521 gcagacccag cgctaccagc aggttcatac aggcgattgc tgcggcaccc gcgccggaaa
   107581 ccaccatccg cacgtcggag atgtttttct ccaccacgcg caagccgttg aggatggcgg
   107641 cagtgctgat gattgccgtg ccgtgctgat cgtcgtggaa taccggaata ttcatccgct
   107701 cgcgcagttt ctgttcaata tagaaacatt ctggcgcttt aatgtcttcg aggttgattc
   107761 cgccgaaggt cggttcgagc gcagcgacaa cttcaataaa tttgtccggg tcgagttcat
   107821 caacttcaat gtcaaataca tcaatcccgg cgaatttctt aaacagaacg cccttgcctt
   107881 ccatcaccgg tttgccagcc agcgcgccaa tgttgcctaa ccccagcacc gccgtaccgt
   107941 tagagatcac cgccaccagg ttaccgcgcg cggtatattt gtaggctttt aacgggtctt
   108001 tttcgatttc aagacaaggt gcggcaacgc ctggtgagta ggccagcgcc agatcgcgct
   108061 gcgttgccag aggcttggtt ggagaaacct ggattttccc tggaactgga aattcatgga
   108121 aatcaagtgc actttgtttt aactggtcat ccatttggtt gttcctttca cgtaacgttc
   108181 acaaataaag tgtgtgggca acagcccctg cccacaacgt ggcgcacatt attaccctgc
   108241 cggagtctac agactttgag caagtccaaa ctctcaccat taatataatg ttttggtaat
   108301 aatcttataa cactgatgtt acctgcttaa tccagcaata ccatgcctgt ctgctatgct
   108361 tttttgatgc gtttagcgaa atttctcaga agtgtgaatt aacgcactca tctaacactt
   108421 tacttttcaa ggagtatttc ctatgaacga gttagacggc atcaaacagt tcaccactgt
   108481 cgtggcagac agcggcgata ttgagtccat tcgccattat catccccagg atgccaccac
   108541 caatccttcg ctgttactca aagctgccgg attatcacaa tatgagcatt taatagacga
   108601 tgctatcgcc tggggtaaaa aaaatggcaa gacccaggaa caacaggtgg tcgcagcgtg
   108661 tgacaaactg gcagtcaatt tcggtgctga aattctcaaa atcgtacccg gtcgcgtgtc
   108721 aacagaagtt gatgcacgcc tctcttttga taaagaaaag agtattgaga aggcgcgcca
   108781 tctggtggac ttgtatcagc aacaaggcgt tgagaaatca cgcattttga tcaaactggc
   108841 ttcgacctgg gaaggaattc gcgcggccga agagctggaa aaagaaggta ttaactgcaa
   108901 cctgacgctg ctgttttctt ttgcccaggc gcgggcctgt gcagaagcgg gcgtttttct
   108961 gatttcgccg tttgtcgggc gtatttatga ctggtatcag gcacgcaagc cgatggaccc
   109021 gtatgtggtg gaagaagatc cgggcgttaa atcggtgcgc aatatctacg actactataa
   109081 gcaacaccac tatgaaacca ttgtgatggg cgcgagcttc cgtcgcaccg aacaaatcct
   109141 cgccttaacc ggctgcgatc gactgactat cgcaccgaat ttgctgaagg agctgcagga
   109201 aaaagtttcg ccagtggtac gtaaattaat cccgccttct cagacgttcc cacgcccagc
   109261 acccatgagc gaagcggagt tccgttggga gcacaatcag gatgcgatgg cggtagaaaa
   109321 actgtctgaa ggcattcgtc tattcgccgt tgatcaacgc aaactggaag atcttcttgc
   109381 cgccaaacta taaaccagcc acggagtgtt atatgtcccg aaaagacctt gccaatgcga
   109441 ttcgcgcact cagtatggat gcggtacaaa aagccaattc tggtcatccc ggcgcgccga
   109501 tgggcatggc tgatattgcc gaagtgctgt ggaacgattt tcttaaacat aatcctaccg
   109561 acccaacctg gtatgatcgc gaccgcttta ttctttccaa cggtcacgcg tcgatgctgc
   109621 tctacagttt gctacatctg accggttacg acctgccgct ggaagaactg aagaacttcc
   109681 gtcagttgca ttcgaaaacc ccaggccacc cggagattgg ctatacgcca ggcgttgaaa
   109741 ccaccaccgg cccgcttgga caaggtttgg cgaacgccgt cgggctggcg atagcggaac
   109801 gtacactggc ggcgcagttt aaccagccag accatgagat cgtcgatcac ttcacctatg
   109861 tgtttatggg cgacggctgc ctgatggaag gtatttccca cgaagtctgt tcgctggcag
   109921 gcacgctggg actgggcaag ctgattggtt tttacgatca caacggtatt tccatcgacg
   109981 gtgaaacaga aggctggttt accgacgata cggcaaaacg ttttgaagcc tatcactggc
   110041 atgtgatcca tgaaatcgac ggtcacgatc cgcaggcggt gaaggaagcg atccttgaag
   110101 cgcaaagcgt gaaagataag ccgtcgctga ttatctgccg tacggtgatt ggctttggtt
   110161 cgccgaataa agcaggtaag gaagaggcgc acggcgcacc actgggggaa gaagaagtgg
   110221 cgctggcacg gcaaaaactg ggctggcacc atccgccatt tgagatccct aaaaagattt
   110281 atcacgcctg ggatgcccgt gaaaaaggcg aaaaagcgca gcagagctgg aatgagaagt
   110341 ttgccgccta taaaaaggct catccgcaac tggcagaaga gtttacccga cggatgagcg
   110401 gtggtttacc gaaggactgg gagaaaacga ctcagaaata tatcaatgag ttacaggcaa
   110461 atccggcgaa aatcgctacc cgtaaggctt cgcaaaatac gcttaacgct tacgggccga
   110521 tgctgcctga gttgctcggc ggttcggcgg atctggctcc cagcaacctg accatctgga
   110581 aaggttctgt ttcgctgaag gaagatccag cgggcaacta cattcactac ggggtgcgtg
   110641 aatttggcat gaccgctatc gccaacggca tcgcgcacca cggcggcttt gtgccgtata
   110701 ccgcgacgtt cctgatgttt gttgaatacg cccgtaacgc cgcgcggatg gcggcactga
   110761 tgaaagcgcg gcagattatg gtttataccc acgactcaat tggcctgggc gaagatggtc
   110821 cgacgcacca ggctgttgag caactggcca gcctgcgctt aacgccaaat ttcagcacct
   110881 ggcgaccgtg cgatcaggtg gaagcggcgg tgggctggaa gctggcggtt gagcgccaca
   110941 acggaccgac ggcactgatc ctctcaaggc agaatctggc ccaggtggaa cgtacgccgg
   111001 atcaggttaa agagattgct cgtggcggtt atgtgctgaa agacagcggc ggtaagccag
   111061 atattattct gattgccacc ggttcagaga tggaaattac cctgcaagcg gcagagaaat
   111121 tagcaggaga aggtcgcaat gtacgcgtag tttccctgcc ctcgaccgat attttcgacg
   111181 cccaggatga ggaatatcgg gagtcggtgt tgccttctaa cgttgcggct cgcgtggcgg
   111241 tggaagcagg tattgccgat tactggtaca agtatgttgg tctgaaaggg gcaattgtcg
   111301 ggatgacggg ttacggggaa tctgctccgg cggataagct gttcccgttc tttggcttta
   111361 ccgccgagaa tattgtggca aaagcgcata aggtgctggg agtgaaaggt gcctgatggt
   111421 gatcgccgga cgctgattgc cggatgcgac gctgacgcgt cttatccggc ctacatgtcc
   111481 ccgccatttt gtttaacggg tgatccacaa cgtgggccag gcatctggcc catgccagtt
   111541 atcgcaggtc ggttctgcgg cgtaacgcac caggcgaaaa cgctgaccgt caaagcgcca
   111601 gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca ccagctcacg
   111661 cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct gaccgttgtt
   111721 gaacggcaaa cgcaaccgaa ccgggcgcga ggccagcggc tttttacgcg acacaatcca
   111781 tgccaaatca atggtgttat aggcccctgc ctcacagcta atcatcatca gcgctttatc
   111841 atcagtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc agcgcagacc
   111901 attcatccgc cagtttccat aatccagcaa atcgttgcgt tcttcgagtg agagtggcgt
   111961 cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta cgctgagcgg
   112021 cggttcgtcc cctttcttga tccaggcggt ttcactgcca acgcgctttt gctgagcatc
   112081 aataaacaac aacgccgctt ttaagccgct cagagaaatg gtctgattgc cgtcgcgtaa
   112141 ggtgatcgcc ttcccttcct gaattatctg caaaaacgcg gtgatggttg ccgtatcatc
   112201 ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaacgcca acggctcacc
   112261 atctaacagc agccgtggcg ctatctcccc ttcagacgca tccggcgact tcaacccgcc
   112321 gcgctcaata cgtaaaacgg catcggtatg cgccccggcg ctgcggctca gggtcatcac
   112381 cagtccatta tgatcgcccg tattgcgcgc cacacaaaaa ttttgattat tacaggtgac
   112441 ctgccagtcg gaaaacgccc gttgcgctgg tgccgcccac accaaagacg tcggcaacaa
   112501 agcgaaaaaa agagaaggaa aatgcgatag cgcatggacg gtacgacccc agaagttcaa
   112561 acaaacagca caagtcgtat cttcgttgcg caggtagcgc ggctcaatcg gatttgtcgg
   112621 attgagccaa caataaacaa cgaaatatta ttgttatttt tcagtccatt aaatgtgacg
   112681 tttgcaaata gttaagcaat aacaccgtct taccgtcacg tatctcgccg gttttgatca
   112741 tctccagcgc ctggctgaac ggcagctcga gcacttcaat atcttcatct tcaacaccgc
   112801 caccggcgtt agcgcgctga ttgtcactgt attcggcgat aaaaaagtgg attagctcag
   112861 tcacaccgcc tggcgacata tacagttcaa ataatttgcg cacttcgccc acttcatagc
   112921 ccgtctcttc aatcacttct ttgcgaatgc acacttccgg ttcgtcgtta tccagcagcc
   112981 cggcgcaggt ttcaatcagc tgcccgcttt cattgccatt aacccaggta gcgacacgga
   113041 actgacgaat cagaaccacg gtctttttct tcgcgttgta caggaggatc gtcgcaccat
   113101 taccgcgatc gtaaacttca cgtttatggc ggataacttc gccatctttg cgcgtgagat
   113161 cgtaggtaat gttgtgcagg gtgaaatagt tatcggagag aattttgtct ttaatgaggg
   113221 tgatttgttg cgtcataccg actccacagc gcgaaatgaa caattatatt acgctgtgaa
   113281 gtcgggtttg tctgcgcagg ctatcagtga gatttgactg attttacccc cagccagtca
   113341 ataatcccct gtgccgcatg acgtccttct gccattgcgg taaccaccag atccgcacca
   113401 cgcacggcgt cgccaccggc gaagattttc ggattggtgg tctggtaacg gtactggctt
   113461 tccacatccg cgatgatgcg gccccatttg tctaccgtta caccgtgcga ctccagccac
   113521 ggcatcccgt gcggattgaa gccaaacgcc ataatcaccg cgtcggctgg catgacaaat
   113581 tcactgcctt ccaccggtac tggatgccga cgcccctggg catccggctc gccaagacgc
   113641 gtacgcagga aacgaatccc gcaaacgtga ccttgttcat tcagctcaag cgccaccggc
   113701 tggacgttaa attcgaagtt ggccccctct tcgcgggcgt tcttcacctc tttcttcgag
   113761 cctggcatgt tagcttcatc acgacgataa gcgcaggtga cgttactcgc gccgtggcgc
   113821 agcgcagtac gcacacagtc catcgcggtg tcgccgccgc ccagtaccac gacgttaagt
   113881 ccggcggtat tgataaacgg ctcttccggt agctcttcga gtcccatcac ctgtttagtg
   113941 ttggcaatga ggaacggcag cgcgtcataa acgcccggcg catcttcatt gggtaaaccc
   114001 gctttcatgg aacggtaagt gcctacgcca acgaagaccg cgtcgtattg ttccaaaagc
   114061 gaatccaaag agacatcttt acccacttca caattgagtt cgaagtgaat ccccatcgcg
   114121 ctgaagattt cccgacggcg tgccagcagg gatttatcca gtttgaaaga aggaatgccg
   114181 aaagtgagca agccaccgat ttctggatgg cgatcgtaca ccgtcaccgc cacgccattg
   114241 cgggtcagaa catccgcaca ggccagcccc gccggacctg ccccgataat cgccacccgc
   114301 ttgtccactt tggtgacatg acttaagtca ggacgccaac ctttcgccaa cgcctgatct
   114361 gaaatatagc gttcaatgtt gccgatagtt accgcaccgt gctcatcgcg aatagtacag
   114421 gcaccttcac acaaacggtc ttgcggacaa acgcgtccgg taatttccgg cagggtgttg
   114481 gtctggtgag aaagctcgac ggcggcgtcg atgtttccgg ctttcaccag ttcaatccac
   114541 tgcggaatat ggttatgcag cgggcaggtc cattcacaaa cgctgtgctc gccgcactta
   114601 aggcagcgcg aggcttcccg ttgtgcctgg tcggcgcgaa atggcagata aatttcatca
   114661 aaaccggttt tgcgcgcttc aatcgccagt ttatccggct cgccacgcgc gggcgttgcc
   114721 tgcatttgtt cgactttact cattaccggc atttcttgcg ccgcggtact ggcatgccac
   114781 ggttgatgtt cctgacgcgc ggtgcgcaag cggcgggatt tcgccatgcc ggagagtgcg
   114841 acgtcagtga ccagttgcag cgcgtccgcc gggcaattct caacacaggc aggaccgttt
   114901 tcgcgccctg cacaaaggtc gcatttatgc gccgtggctt ttacttttcc tgccgcgacg
   114961 ggtgtcagga cgatttgcat cgtaccaaac ggacaggcca ccacgcagga tttacagcca
   115021 atacactttt gctgattgac ctgaatgctg tcatcaacgt ggctgattgc gccattaggg
   115081 cagctacggg cgcagggcgc atcttcacaa tggtgacagg tcactgcact acgttgctgt
   115141 tgatgtttga taaccgtaat tcggggatga aaatggtgtt ggctcaggac atgttgctca
   115201 tcattgtgag ccatgacaca ggcgatttca caagcatgac aacccagaca ttgctgactg
   115261 ttggccataa taaaacgatt cataacgacc ttcttttttg gttgtaaaaa ccttattctt
   115321 tatatgagtg ttgttattac ccgacttaca ggggaatcgg caatgttcat gtgcccagaa
   115381 taagtaacta tttcgctata aactgtggca gatcaaataa tcccatcact gactaaattg
   115441 tgtttcagct aactggaaca ttaatgattt tttgtggaga agacgcgtgt gattgttaaa
   115501 cgacccgtct cggccagtct ggcccgggcc tttttttaca ttgtgctgct gtcgattctt
   115561 tccacgggta tcgctctgct aactctggcg agcagtttgc gcgacgctga ggctatcaat
   115621 attgccggat cgctacgtat gcagagttac cgcctgggct acgacttgca aagtggcagt
   115681 ccacaactca atgcacatcg ccagttgttt cagcaggcac tgcattcacc ggtattaacc
   115741 aatctcaacg tctggtatgt gccagaagca gtaaaaactc gctatgcgca tctgaatgcc
   115801 aactggctgg agatgaataa tcggctcagc aagggcgatt tgccgtggta tcaggccaat
   115861 attaataatt atgttaatca gatagacctg ttcgtgctgg ctttacagca ctacgctgaa
   115921 cgcaaaatgc tgctggtggt ggcgatttcc ctggctggcg gtatcggtat tttcacgctg
   115981 gtctttttta ctttgcgccg tatacgccat caggtggttg ccccgctgaa tcagctggtt
   116041 accgccagtc aacgtattga acacgggcag ttcgactcgc cgccgctgga taccagtctg
   116101 ccgaatgagc ttggtctgct tgcaaaaacc tttaaccaga tgtcgagcga gctgcataaa
   116161 ttgtaccttt cgctggaggc gtcagtagaa gaaaagaccc gcgatctcca cgaggccaag
   116221 cgtcgtctgg aggtgttgta tcaatgttcc caggcgctga acaccagcca gattgatgtg
   116281 cattgtttcc gccatatttt gcagattgtt cgcgacaatg aagcggctga atatctggag
   116341 ttaaatgtcg gtgaaaactg gcggattagc gaagggcaac caaacccgga attgccgatg
   116401 cagattttac cggtgacaat gcaagagacg gtttacggcg aactgcactg gcaaaatagt
   116461 cacgtttcat catcagaacc gctgcttaac agtgtttcgt cgatgctggg acgcggtttg
   116521 tactttaatc aggcgcagaa gcattttcag cagttattgt tgatggaaga acgtgcaacc
   116581 atcgcccgcg aattgcacga ctcgctggct caggtacttt cttacttacg tatccagttg
   116641 acgttactga agcgttcgat accggaagat aacgccaccg cacaaagtat catggccgat
   116701 ttttcccagg cgttgaatga tgcttatcgg cagttacgcg agctgttgac tactttccgc
   116761 ctgacgctgc agcaggcgga tctcccctcc gcgttgaggg aaatgctgga tacgttacaa
   116821 aatcaaacca gcgccaaact gaccctcgac tgccgtctgc caaccctggc gctggatgcg
   116881 caaatgcagg tgcatttgtt gcaaattatt cgcgaagcgg tgctgaatgc gatgaagcac
   116941 gccaacgcca gcgaaatcgc cgtcagttgc gtcaccgcgc cggacggcaa tcacacggtt
   117001 tatatccgtg ataacgggat tggcatcggt gaaccgaaag agcccgaagg tcattatggt
   117061 ctgaatatca tgcgcgaacg cgcggaacgg ctgggtggga cgctgacttt ttcacaacct
   117121 tccggcggcg gcacgttagt gagtattagc tttcgctccg cggagggtga ggaaagtcag
   117181 ctgatgtaat gcctcctact gaccaaagaa tacttgcact taaggttcag tataaaaggg
   117241 catgataatt tacattaact cctttttttc tccacgattg gctcgtacct tgccgctaca
   117301 gtgaagcaag tcaagcctac aacgatacgc agaaacacga ggtcctcttt taatggcgaa
   117361 tttctttatt gatcgcccca tttttgcctg ggtgctggca atcctgttgt gtctgacagg
   117421 taccctggcg attttctcat tgcccgttga acaatacccc gatctcgcgc caccgaatgt
   117481 gcgagtgacc gctaactatc ccggcgcatc ggcccagacg ctggaaaaca ccgtgaccca
   117541 ggttatcgag caaaatatga ccggcctcga taatctcatg tatatgtcat ctcagagcag
   117601 tggcaccggt caggcatctg tcactttaag ttttaaagca ggcaccgatc cggacgaagc
   117661 cgtgcagcaa gtacaaaacc agctgcaatc agccatgcga aagttaccgc aggcggtgca
   117721 aaatcagggc gtgacggtgc gtaaaaccgg cgataccaat attctgacca ttgctttcgt
   117781 ctctaccgat ggttcgatgg ataaacagga tattgccgat tatgttgcca gtaatattca
   117841 ggacccgtta agccgcgtga atggcgtcgg ggatatcgat gcctatggtt cgcaatattc
   117901 catgcgtatc tggctggacc cggcgaaact caacagtttc cagatgacgg ctaaagatgt
   117961 cactgatgcc attgagtcac agaacgcgca gattgcggtt gggcaacttg gtggtacacc
   118021 ttccgtcgat aagcaggcgc tcaacgccac cattaacgcc cagtcactgc tgcaaacacc
   118081 agaacagttc cgcgatatca ccttgcgggt gaatcaagac ggctcagagg taaggctggg
   118141 cgatgtcgcc accgtcgaaa tgggggcgga gaaatacgat tatcttagcc gcttcaatgg
   118201 taagccagcc tccgggctgg gggtaaaact ggcctccggc gcaaacgaaa tggcgacagc
   118261 ggagctggtg ctcaatcgtc tcgacgagct ggcgcagtac ttcccgcacg gactggaata
   118321 caaggtggcg tatgaaacca cctcgtttgt taaagcctcc attgaagacg tggtgaaaac
   118381 gctgctggaa gctatcgctc tggttttcct cgttatgtat ctgttcctgc aaaacttccg
   118441 cgccacgctg atacccacta tcgccgtgcc ggtggtgttg atgggaacct tctccgtact
   118501 ttacgccttc ggttacagcg tcaacacctt aaccatgttc gcgatggtac tggcgatcgg
   118561 tctgctggtg gatgacgcca tcgtggtggt ggaaaacgtc gaacgtatta tgagtgagga
   118621 aggactcact cctcgcgaag ccacacgtaa atcgatgggg cagatccagg gggcactggt
   118681 cgggattgcg atggtgcttt cggcggtatt tgtgccaatg gccttcttcg gcggcaccac
   118741 cggtgccatc tatcgccagt tctctattac cattgttgca gcgatggtgt tgtcagtact
   118801 ggtagcgatg atcctcactc cggctctgtg tgccacgcta cttaagccac tgaaaaaagg
   118861 tgaacatcac gggcaaaaag gcttttttgc ctggtttaac cagatgttta accgcaacgc
   118921 cgaacgctac gaaaaagggg tggcgaaaat tctccaccgt agcctgcgct ggattgtgat
   118981 ttatgtcctg ctgcttggcg gcatggtgtt cctgttcctg cgtttgccga cgtcgttctt
   119041 accgctggaa gaccggggca tgtttactac ctcggtacaa ttgcccagcg gttcaaccca
   119101 acaacagacc ctgaaagtcg ttgagcaaat cgagaaatat tacttcaccc atgaaaaaga
   119161 caacatcatg tcggtgtttg ccaccgttgg ttctggccct gggggtaacg ggcaaaacgt
   119221 ggcgcgaatg tttatccgcc tgaaagactg gagcgaacgc gacagtaaga ccggcacctc
   119281 gtttgccatt atcgagcgtg caacgaaggc gtttaaccaa attaaagaag ctcgcgttat
   119341 cgccagcagc ccgccagcaa ttagcggtct tggtagttct gcgggttttg atatggagtt
   119401 gcaggaccac gctggagcag gtcacgatgc gctgatggca gcacgtaacc agttgctggc
   119461 gctggcggcg gaaaacccgg agctaacccg tgtgcgccat aacggcctcg atgacagtcc
   119521 gcagttgcag attgatatcg accagcgtaa agctcaggcg ctgggcgttg ctatcgacga
   119581 tattaacgac acactgcaaa ccgcctgggg ttcgagctat gtgaatgact ttatggatcg
   119641 cggtcgcgtg aagaaagtct atgtgcaggc agctgcgccg tatcgcatgc tgccagatga
   119701 catcaatctc tggtatgtcc gaaataaaga tggcggcatg gtgcccttct ctgctttcgc
   119761 gacctcacgc tgggaaacag gctcgccgcg tctggaacgc tataacggtt attctgcggt
   119821 tgagattgtt ggggaagccg caccgggggt cagtaccggt acggcgatgg atattatgga
   119881 atcgttagtg aagcagctgc caaacggctt tggtctggag tggacggcga tgtcgtatca
   119941 ggagcggctt tccggcgcgc aggctccggc gctgtacgcc atttccttgc tggtggtatt
   120001 cctgtgtctg gctgcgttgt atgaaagctg gtcggtgccg ttctcggtaa tgctggtcgt
   120061 gccgctgggg gtaatcggcg cgctgctggc aacctggatg cgcgggctgg aaaacgacgt
   120121 ttacttccag gtgggcctgt taacggtcat tggtttatcg gcgaaaaacg ccatcctgat
   120181 cgtcgagttt gctaacgaga tgaaccaaaa aggccacgac ctgtttgaag cgacgctcca
   120241 cgcctgccgt cagcgtttac gcccgattct gatgacctcg ctggcattta tcttcggcgt
   120301 attgccaatg gcaaccagca cgggtgccgg ttccggtggt cagcatgcgg tgggtactgg
   120361 cgtaatgggc gggatgattt cagctactat tctggctatt tacttcgtgc cgctgttctt
   120421 tgtgctggtg cgccgccgct tcccgctgaa gccgcgcccg gaataagcaa taaaaaaggc
   120481 gacatgccaa tgtgtcgcct ttttcaactt tccgattaag aacctgctca gcgggttctt
   120541 gctgtttata ctttgtctcg ggaattactt acgaagcata acttcgataa agtctttcca
   120601 gttccccagt tcacgttcaa tcataacaac ctctcttata attatgggta ttctacggaa
   120661 acaatatacc gtggtgaagc taatttactc gattgctgcg atgactacct ccgggggaca
   120721 aaccttatgt aaatactatg gtcctgcggt gataatttgt atgtgataca cagcaacatt
   120781 tcgagatatt catacggcat ctaatactga tttaattctg gttaaaatac
//
LOCUS       DAZGXZ010000002       117967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000002 DAZGXZ010000000
VERSION     DAZGXZ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 117967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 117967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..117967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.002"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHKJF_000130"
     CDS             complement(<1..209)
                     /locus_tag="ACHKJF_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0947558.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKJF_000131"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKJF_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP0947559.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKJF_000132"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKJF_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP0947560.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKJF_000133"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKJF_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP0947561.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKJF_000134"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKJF_000134"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP0947562.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKJF_000135"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKJF_000135"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP0947563.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDQQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKJF_000136"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKJF_000136"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP0947564.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKJF_000137"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKJF_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0947565.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKJF_000138"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKJF_000138"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0947566.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKJF_000139"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKJF_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP0947567.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKJF_000140"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKJF_000140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0947568.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKJF_000141"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKJF_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP0947569.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKJF_000142"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKJF_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP0947570.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKJF_000143"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKJF_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP0947571.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKJF_000144"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKJF_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0947572.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKJF_000145"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKJF_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0947573.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRRLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKJF_000146"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKJF_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP0947574.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKJF_000147"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKJF_000147"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP0947575.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKJF_000148"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKJF_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP0947576.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKJF_000149"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKJF_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP0947577.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKJF_000150"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKJF_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP0947578.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKJF_000151"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKJF_000151"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP0947579.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKJF_000152"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKJF_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP0947580.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKJF_000153"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKJF_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP0947581.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKJF_000154"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKJF_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP0947582.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKJF_000155"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHKJF_000155"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP0947583.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21449..22630
                     /gene="tsgA"
                     /locus_tag="ACHKJF_000156"
     CDS             21449..22630
                     /gene="tsgA"
                     /locus_tag="ACHKJF_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP0947584.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDARVAKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22892..25435
                     /gene="nirB"
                     /locus_tag="ACHKJF_000157"
     CDS             22892..25435
                     /gene="nirB"
                     /locus_tag="ACHKJF_000157"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP0947585.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25432..25758
                     /gene="nirD"
                     /locus_tag="ACHKJF_000158"
     CDS             25432..25758
                     /gene="nirD"
                     /locus_tag="ACHKJF_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP0947586.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="ACHKJF_000159"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="ACHKJF_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP0947587.1"
                     /translation="MFTDTINKCAANAARIERLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cysG"
                     /locus_tag="ACHKJF_000160"
     CDS             26709..28082
                     /gene="cysG"
                     /locus_tag="ACHKJF_000160"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP0947588.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28329..28496
                     /gene="yhfL"
                     /locus_tag="ACHKJF_000161"
     CDS             28329..28496
                     /gene="yhfL"
                     /locus_tag="ACHKJF_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP0947589.1"
                     /translation="MNKFIKVALVGAVLATLTACTDHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28793..30130
                     /gene="frlA"
                     /locus_tag="ACHKJF_000162"
     CDS             28793..30130
                     /gene="frlA"
                     /locus_tag="ACHKJF_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP0947590.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFASPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPNVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30151..31173
                     /gene="frlB"
                     /locus_tag="ACHKJF_000163"
     CDS             30151..31173
                     /gene="frlB"
                     /locus_tag="ACHKJF_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP0947591.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKLLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPDVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYTEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31223..32053
                     /gene="frlC"
                     /locus_tag="ACHKJF_000164"
     CDS             31223..32053
                     /gene="frlC"
                     /locus_tag="ACHKJF_000164"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP0947592.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYMLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32050..32835
                     /gene="frlD"
                     /locus_tag="ACHKJF_000165"
     CDS             32050..32835
                     /gene="frlD"
                     /locus_tag="ACHKJF_000165"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP0947593.1"
                     /translation="MKPLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAQTIQYHGAW"
     gene            32935..33666
                     /gene="frlR"
                     /locus_tag="ACHKJF_000166"
                     /gene_synonym="yhfR"
     CDS             32935..33666
                     /gene="frlR"
                     /locus_tag="ACHKJF_000166"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP0947594.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERISAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33818..34903)
                     /gene="yhfS"
                     /locus_tag="ACHKJF_000167"
     CDS             complement(33818..34903)
                     /gene="yhfS"
                     /locus_tag="ACHKJF_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0947595.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPCITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAASVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34915..36219)
                     /gene="yhfT"
                     /locus_tag="ACHKJF_000168"
     CDS             complement(34915..36219)
                     /gene="yhfT"
                     /locus_tag="ACHKJF_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP0947596.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36231..36584)
                     /gene="yhfU"
                     /locus_tag="ACHKJF_000169"
     CDS             complement(36231..36584)
                     /gene="yhfU"
                     /locus_tag="ACHKJF_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP0947597.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36595..37473)
                     /gene="php"
                     /locus_tag="ACHKJF_000170"
     CDS             complement(36595..37473)
                     /gene="php"
                     /locus_tag="ACHKJF_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP0947598.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNCYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKHIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37470..38696)
                     /gene="yhfW"
                     /locus_tag="ACHKJF_000171"
     CDS             complement(37470..38696)
                     /gene="yhfW"
                     /locus_tag="ACHKJF_000171"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0947599.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDHVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38696..39859)
                     /gene="yhfX"
                     /locus_tag="ACHKJF_000172"
     CDS             complement(38696..39859)
                     /gene="yhfX"
                     /locus_tag="ACHKJF_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP0947600.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRADLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(39943..40305)
                     /gene="yhfY"
                     /locus_tag="ACHKJF_000173"
     CDS             complement(39943..40305)
                     /gene="yhfY"
                     /locus_tag="ACHKJF_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0947601.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40322..41227)
                     /gene="yhfZ"
                     /locus_tag="ACHKJF_000174"
     CDS             complement(40322..41227)
                     /gene="yhfZ"
                     /locus_tag="ACHKJF_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP0947602.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHSLLAHQQRVVSGEQEPSY"
     gene            complement(41496..42500)
                     /gene="trpS"
                     /locus_tag="ACHKJF_000175"
     CDS             complement(41496..42500)
                     /gene="trpS"
                     /locus_tag="ACHKJF_000175"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP0947603.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42493..43251)
                     /gene="gph"
                     /locus_tag="ACHKJF_000176"
     CDS             complement(42493..43251)
                     /gene="gph"
                     /locus_tag="ACHKJF_000176"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP0947604.1"
                     /translation="MSKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43244..43921)
                     /gene="rpe"
                     /locus_tag="ACHKJF_000177"
     CDS             complement(43244..43921)
                     /gene="rpe"
                     /locus_tag="ACHKJF_000177"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP0947605.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43939..44775)
                     /gene="dam"
                     /locus_tag="ACHKJF_000178"
     CDS             complement(43939..44775)
                     /gene="dam"
                     /locus_tag="ACHKJF_000178"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0947606.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44882..46168)
                     /gene="damX"
                     /locus_tag="ACHKJF_000179"
     CDS             complement(44882..46168)
                     /gene="damX"
                     /locus_tag="ACHKJF_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP0947607.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46260..47348)
                     /gene="aroB"
                     /locus_tag="ACHKJF_000180"
     CDS             complement(46260..47348)
                     /gene="aroB"
                     /locus_tag="ACHKJF_000180"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP0947608.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47405..47926)
                     /gene="aroK"
                     /locus_tag="ACHKJF_000181"
     CDS             complement(47405..47926)
                     /gene="aroK"
                     /locus_tag="ACHKJF_000181"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP0947609.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48327..49565)
                     /gene="hofQ"
                     /locus_tag="ACHKJF_000182"
     CDS             complement(48327..49565)
                     /gene="hofQ"
                     /locus_tag="ACHKJF_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP0947610.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49477..49881)
                     /gene="hofP"
                     /locus_tag="ACHKJF_000183"
     CDS             complement(49477..49881)
                     /gene="hofP"
                     /locus_tag="ACHKJF_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP0947611.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49871..50311)
                     /gene="hofO"
                     /locus_tag="ACHKJF_000184"
     CDS             complement(49871..50311)
                     /gene="hofO"
                     /locus_tag="ACHKJF_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP0947612.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50295..50834)
                     /gene="hofN"
                     /locus_tag="ACHKJF_000185"
     CDS             complement(50295..50834)
                     /gene="hofN"
                     /locus_tag="ACHKJF_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP0947613.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50834..51613)
                     /gene="hofM"
                     /locus_tag="ACHKJF_000186"
     CDS             complement(50834..51613)
                     /gene="hofM"
                     /locus_tag="ACHKJF_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP0947614.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51733..54285
                     /gene="mrcA"
                     /locus_tag="ACHKJF_000187"
     CDS             51733..54285
                     /gene="mrcA"
                     /locus_tag="ACHKJF_000187"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP0947615.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNIVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQLARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54450..55010)
                     /gene="nudE"
                     /locus_tag="ACHKJF_000188"
     CDS             complement(54450..55010)
                     /gene="nudE"
                     /locus_tag="ACHKJF_000188"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP0947616.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55330..57465
                     /gene="igaA"
                     /locus_tag="ACHKJF_000189"
     CDS             55330..57465
                     /gene="igaA"
                     /locus_tag="ACHKJF_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP0947617.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57530..58198
                     /gene="yrfG"
                     /locus_tag="ACHKJF_000190"
     CDS             57530..58198
                     /gene="yrfG"
                     /locus_tag="ACHKJF_000190"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP0947618.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58209..58610
                     /gene="hslR"
                     /locus_tag="ACHKJF_000191"
     CDS             58209..58610
                     /gene="hslR"
                     /locus_tag="ACHKJF_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP0947619.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58635..59513
                     /gene="hslO"
                     /locus_tag="ACHKJF_000192"
     CDS             58635..59513
                     /gene="hslO"
                     /locus_tag="ACHKJF_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP0947620.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59648..61372)
                     /gene="yhgE"
                     /locus_tag="ACHKJF_000193"
     CDS             complement(59648..61372)
                     /gene="yhgE"
                     /locus_tag="ACHKJF_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP0947621.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            61751..63373
                     /gene="pckA"
                     /locus_tag="ACHKJF_000194"
     CDS             61751..63373
                     /gene="pckA"
                     /locus_tag="ACHKJF_000194"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP0947622.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPIQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            63490..63807
                     /locus_tag="ACHKJF_000195"
     CDS             63490..63807
                     /locus_tag="ACHKJF_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0947623.1"
                     /translation="MFTFIELQGFSKRRQLLLPDDEFRIFQELLIQDPTAGDIIAGTG
                     SFRKIRWNRSGMGKRGGIRIIYYNVTRNGRIYLALIYPKNEQDDLTEEQRKALKLLSE
                     KLL"
     gene            63866..64162
                     /gene="nadS"
                     /locus_tag="ACHKJF_000196"
     CDS             63866..64162
                     /gene="nadS"
                     /locus_tag="ACHKJF_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0947624.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSWETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(64193..65545)
                     /gene="envZ"
                     /locus_tag="ACHKJF_000197"
     CDS             complement(64193..65545)
                     /gene="envZ"
                     /locus_tag="ACHKJF_000197"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP0947625.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(65542..66261)
                     /gene="ompR"
                     /locus_tag="ACHKJF_000198"
     CDS             complement(65542..66261)
                     /gene="ompR"
                     /locus_tag="ACHKJF_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP0947626.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            66489..66965
                     /gene="greB"
                     /locus_tag="ACHKJF_000199"
     CDS             66489..66965
                     /gene="greB"
                     /locus_tag="ACHKJF_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP0947627.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            67062..69383
                     /gene="yhgF"
                     /locus_tag="ACHKJF_000200"
     CDS             67062..69383
                     /gene="yhgF"
                     /locus_tag="ACHKJF_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP0947628.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFEEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQTAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMSKKR"
     gene            69821..70048
                     /gene="feoA"
                     /locus_tag="ACHKJF_000201"
     CDS             69821..70048
                     /gene="feoA"
                     /locus_tag="ACHKJF_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP0947629.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            70065..72386
                     /gene="feoB"
                     /locus_tag="ACHKJF_000202"
     CDS             70065..72386
                     /gene="feoB"
                     /locus_tag="ACHKJF_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP0947630.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALDRLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFMGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            72386..72622
                     /gene="feoC"
                     /locus_tag="ACHKJF_000203"
     CDS             72386..72622
                     /gene="feoC"
                     /locus_tag="ACHKJF_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP0947631.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72825..73703
                     /gene="rpnA"
                     /locus_tag="ACHKJF_000204"
     CDS             72825..73703
                     /gene="rpnA"
                     /locus_tag="ACHKJF_000204"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP0947632.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(73732..74502)
                     /gene="bioH"
                     /locus_tag="ACHKJF_000205"
     CDS             complement(73732..74502)
                     /gene="bioH"
                     /locus_tag="ACHKJF_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP0947633.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            74540..75223
                     /gene="gntX"
                     /locus_tag="ACHKJF_000206"
     CDS             74540..75223
                     /gene="gntX"
                     /locus_tag="ACHKJF_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP0947634.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            75282..75857
                     /gene="nfuA"
                     /locus_tag="ACHKJF_000207"
     CDS             75282..75857
                     /gene="nfuA"
                     /locus_tag="ACHKJF_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP0947635.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            76218..77534
                     /gene="gntT"
                     /locus_tag="ACHKJF_000208"
     CDS             76218..77534
                     /gene="gntT"
                     /locus_tag="ACHKJF_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0947636.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(77579..79663)
                     /gene="malQ"
                     /locus_tag="ACHKJF_000209"
     CDS             complement(77579..79663)
                     /gene="malQ"
                     /locus_tag="ACHKJF_000209"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP0947637.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(79673..82066)
                     /gene="malP"
                     /locus_tag="ACHKJF_000210"
     CDS             complement(79673..82066)
                     /gene="malP"
                     /locus_tag="ACHKJF_000210"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP0947638.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            82678..85383
                     /gene="malT"
                     /locus_tag="ACHKJF_000211"
     CDS             82678..85383
                     /gene="malT"
                     /locus_tag="ACHKJF_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP0947639.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(85426..86442)
                     /gene="rtcA"
                     /locus_tag="ACHKJF_000212"
                     /pseudo
     CDS             complement(85426..86442)
                     /gene="rtcA"
                     /locus_tag="ACHKJF_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
     gene            complement(86446..87672)
                     /gene="rtcB"
                     /locus_tag="ACHKJF_000213"
     CDS             complement(86446..87672)
                     /gene="rtcB"
                     /locus_tag="ACHKJF_000213"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP0947640.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            87861..89230
                     /locus_tag="ACHKJF_000214"
                     /pseudo
     CDS             87861..89230
                     /locus_tag="ACHKJF_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA repair transcriptional activator RtcR family
                     protein"
     gene            complement(89212..89970)
                     /locus_tag="ACHKJF_000215"
     CDS             complement(89212..89970)
                     /locus_tag="ACHKJF_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP0947641.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(89987..90817)
                     /gene="glpG"
                     /locus_tag="ACHKJF_000216"
     CDS             complement(89987..90817)
                     /gene="glpG"
                     /locus_tag="ACHKJF_000216"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP0947642.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(90862..91188)
                     /gene="glpE"
                     /locus_tag="ACHKJF_000217"
     CDS             complement(90862..91188)
                     /gene="glpE"
                     /locus_tag="ACHKJF_000217"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP0947643.1"
                     /translation="MDQFECINVAVAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            91378..92883
                     /gene="glpD"
                     /locus_tag="ACHKJF_000218"
     CDS             91378..92883
                     /gene="glpD"
                     /locus_tag="ACHKJF_000218"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP0947644.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(92937..93542)
                     /locus_tag="ACHKJF_000219"
     CDS             complement(92937..93542)
                     /locus_tag="ACHKJF_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947645.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(93545..94312)
                     /locus_tag="ACHKJF_000220"
     CDS             complement(93545..94312)
                     /locus_tag="ACHKJF_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947646.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(94315..95025)
                     /locus_tag="ACHKJF_000221"
     CDS             complement(94315..95025)
                     /locus_tag="ACHKJF_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947647.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(95041..96549)
                     /locus_tag="ACHKJF_000222"
     CDS             complement(95041..96549)
                     /locus_tag="ACHKJF_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0947648.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(96674..99121)
                     /gene="glgP"
                     /locus_tag="ACHKJF_000223"
     CDS             complement(96674..99121)
                     /gene="glgP"
                     /locus_tag="ACHKJF_000223"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP0947649.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(99140..100573)
                     /gene="glgA"
                     /locus_tag="ACHKJF_000224"
     CDS             complement(99140..100573)
                     /gene="glgA"
                     /locus_tag="ACHKJF_000224"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP0947650.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(100573..101868)
                     /gene="glgC"
                     /locus_tag="ACHKJF_000225"
     CDS             complement(100573..101868)
                     /gene="glgC"
                     /locus_tag="ACHKJF_000225"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP0947651.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(101886..103859)
                     /gene="glgX"
                     /locus_tag="ACHKJF_000226"
     CDS             complement(101886..103859)
                     /gene="glgX"
                     /locus_tag="ACHKJF_000226"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP0947652.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(103856..106042)
                     /gene="glgB"
                     /locus_tag="ACHKJF_000227"
     CDS             complement(103856..106042)
                     /gene="glgB"
                     /locus_tag="ACHKJF_000227"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP0947653.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(106315..107418)
                     /gene="asd"
                     /locus_tag="ACHKJF_000228"
     CDS             complement(106315..107418)
                     /gene="asd"
                     /locus_tag="ACHKJF_000228"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0947654.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            107611..108204
                     /gene="yhgN"
                     /locus_tag="ACHKJF_000229"
     CDS             107611..108204
                     /gene="yhgN"
                     /locus_tag="ACHKJF_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP0947655.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(108250..109545)
                     /locus_tag="ACHKJF_000230"
     CDS             complement(108250..109545)
                     /locus_tag="ACHKJF_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFP0947656.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEQKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(109452..111548)
                     /locus_tag="ACHKJF_000231"
     CDS             complement(109452..111548)
                     /locus_tag="ACHKJF_000231"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFP0947657.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(111688..113028)
                     /gene="gntU"
                     /locus_tag="ACHKJF_000232"
     CDS             complement(111688..113028)
                     /gene="gntU"
                     /locus_tag="ACHKJF_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0947658.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(113032..113559)
                     /gene="gntK"
                     /locus_tag="ACHKJF_000233"
     CDS             complement(113032..113559)
                     /gene="gntK"
                     /locus_tag="ACHKJF_000233"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0947659.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(113698..114693)
                     /gene="gntR"
                     /locus_tag="ACHKJF_000234"
     CDS             complement(113698..114693)
                     /gene="gntR"
                     /locus_tag="ACHKJF_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP0947660.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(114917..115612)
                     /gene="yhhW"
                     /locus_tag="ACHKJF_000235"
     CDS             complement(114917..115612)
                     /gene="yhhW"
                     /locus_tag="ACHKJF_000235"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP0947661.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(115735..116772)
                     /gene="yhhX"
                     /locus_tag="ACHKJF_000236"
     CDS             complement(115735..116772)
                     /gene="yhhX"
                     /locus_tag="ACHKJF_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0947662.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            117105..117593
                     /gene="yhhY"
                     /locus_tag="ACHKJF_000237"
     CDS             117105..117593
                     /gene="yhhY"
                     /locus_tag="ACHKJF_000237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0947663.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            117830..>117967
                     /locus_tag="ACHKJF_000238"
     CDS             117830..>117967
                     /locus_tag="ACHKJF_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP0947664.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQCGHEDEIFTFSL
                     LN"
BASE COUNT        28590 a        31988 c        29552 g        27837 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggttcga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtcgccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taatgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggggtc tgctgatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctggca gcagctgaaa
     4081 aacgccatca gctataaaga agacagccag atcatcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggc gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagtcttccg
     4321 gcctccgttt catcaacaac gccacggcgt aatgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat aaaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcatttact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccacccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct atatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg ctgagctgga gagcagactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcaac cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt ccaccgcagt gatttcaacc ggtaccggac cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 cacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcga taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caatcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gtaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcggtcgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag cttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatccccagc cgcgatgcca
     9421 acggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacgt cgctcatggg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg tgatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacggcttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tagccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcaaggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ctggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtggcac atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgcttgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaataggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctccggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgca ggctgcaacg cgagtttggt catgcgccaa cagctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccgattgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcacgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcgg ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg cagaatagaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcaga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagacgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaattgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ttgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcactgcga caaacgctgg tgctatgttt gcccgtggct gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttgctg acacagctac tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcta aattgcttcc
    16021 cgcatcgctg ctcgccgcca tttttaccct cagtctggca ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gcgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcgtcttatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggcgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cctctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gtactgtggt ttcagctccg cgccaattaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcaatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattaatgat atcaaatgcc gcccccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac gccgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcactg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagccagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtaag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctctagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattcttaaa tagccttcaa
    21241 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21301 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21361 aacatttaaa cagatcacaa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21421 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21481 gattagcttt ctctcttacg cactgaccgg tgcgttggtt attgtcaccg ggatggtgat
    21541 gggaaatatc gccgattatt tcaatctgcc ggtttccagt atgagtaaca ccttcacctt
    21601 cctcaacgcc ggcattttaa tctctatctt cctcaacgca tggctgatgg aaattgtccc
    21661 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21721 gttcagccac agcctggcgc tgttctcggc ggcgatgttc attctcgggg tggtcagtgg
    21781 catcaccatg tcgattggta cattcctggt aacacaaatg tatgaagggc gtcagcgcgg
    21841 ttcccgcctg ttatttaccg actccttctt cagtatggct gggatgattt tcccaatgat
    21901 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21961 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaaca
    22021 tgcaccaaaa acggatgctc gggtagcgaa agaaaagtgg gggatcggcg tactgtttct
    22081 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22141 gtatgccaaa ggcctgggca tgagcctgaa tgatgctggc acgctggtga gtaacttctg
    22201 gatgtcatac atggtcggta tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22261 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22321 aacaccagca catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22381 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22441 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcccgat
    22501 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22561 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22621 ctctcattaa ttgctcatgc cggacggcac tatcgtcgtc cggccttttc ctctcttacc
    22681 ccgctacgtg catctatttc tataaacccg ttcattttgt ctattttttg cacaaacatg
    22741 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22801 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22861 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22921 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22981 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    23041 gtcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23101 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23161 ggtgattcac tccagcgccg ggcgcaccgt tttttatgac aagctgatca tggcgaccgg
    23221 ttcctacccg tggatcccgc caatcaaagg ttctgatact caggactgct ttgtctatcg
    23281 cactattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaagc gcggtgccgt
    23341 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggcattga
    23401 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23461 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23521 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23581 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23641 aacccagtgt ggtctggacg ttgctccgcg tgggggtatc gtcattaatg attcctgcca
    23701 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23761 tggtctggta gcacctggct acaaaatggc gcaggtcgcc gttgaccata ttctcggtag
    23821 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23881 aggcggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctacg tttacctcga
    23941 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    24001 tgcggtactg gtgggcgata ccagcgacta cggtaacctg ctgcaactgg tgctgaacgc
    24061 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24121 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24181 caccaaaggt gatctgattg ctgccatcaa caaaggctgc cacaccgttg cggcgctgaa
    24241 agccgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24301 gaacgcggaa ctggcgaaac aggggatcga agttaacaac aacctgtgcg aacactttgc
    24361 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24421 gctgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccga ccgtcggttc
    24481 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24541 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24601 ttctccgggt ggtgaaatca ccccggaagg gctgatggcg gtaggtcgta tcgcgcgtga
    24661 atttaatctc tacaccaaaa tcaccggctc tcagcgcctg gcgatgtttg gcgcacagaa
    24721 agacgatctg ccggagatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24781 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24841 cgttggcgac agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24901 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24961 taaagacgtg ggtatcatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    25021 cggcatgaaa ccgcgtcatg cggatctgct ggcggcggat atcgatcgcg aaacgctgat
    25081 taaatatctc gaccgcttca tgatgttcta catccgtact gccgacaaac tgacgcgtac
    25141 cgcaccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25201 cgacaaactg gggctgaacg cacatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25261 agtgtgtgag tggactgaaa cggtcaatac gccgtctgcg cagactcgct tcaaacactt
    25321 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25381 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25441 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcgctgtta
    25501 ggtgacgagc aggtagcaat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25561 aacatcgacc cgttcttcga gtccagcgtg ctgtcacgcg ggctgattgc ggaacaccag
    25621 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25681 tgcatggaag acgaacagtt ttccgtcaag cattacgaag cacgagtgaa agacggcgtg
    25741 gtacagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25801 ctgtaataac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25921 ctgcgcgcat tgaacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccgggc acaccatgtt cctcaccttt ggggtgaaag
    26161 cgggcaccat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggtggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgtct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcaa tctggtggtg tctgctggca tttatcgcgt
    26461 ccggctacga gcactccatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc gggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggtc gcggtctcct ctggcggcac ctctccggtt ctggcacgcc
    27121 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgcaac aatgggtgaa cgtcgccgtt
    27241 tctgggaaaa attgttcgtt aacgaccgcc tggcgcagtc gctggccaac aacgatcaga
    27301 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cccaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacaaacgc
    27841 tggtgttcta tatggggctg aatcaggccg cgaccattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27961 tgattgacgg tacgctcaca cagctgggcg aactggcgca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28261 atgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28321 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28381 ctgcatgtac tgatcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28441 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28501 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28561 gtatttgttt ctatatcctt cttgctcccc acccgtaaaa atattttcac tttaatttca
    28621 attggataac cacattcatt agccacagcg cgatcctgtg cgaaattttg tgatcttcct
    28681 ccacattaca taacatcata tgttgttata ttcatcatgc attgtcatgt taccttttaa
    28741 atgactgcaa actctccccc tacaacgtgt tggacaagaa aaaggtatcg ctatgggaag
    28801 ccaggaactc caacgcaagc tcggattttg ggccgttctt gcaatcgccg tcgggacaac
    28861 cgtcggctcc ggtatttttg tatctgtggg tgaagtggca aaagcagcgg gcacgccgtg
    28921 gctgacggtg ctcgcgtttg tcattggcgg gttaattgtg atcccgcaaa tgtgcgtcta
    28981 tgcggaacta tccaccgctt atccggaaaa tggcgcagat tatgtttatc tgaaaaatgc
    29041 cggaagccga ccgctggctt tcctctccgg ctgggccagc ttctgggcca acgatgcgcc
    29101 gtcattgtcg attatggcgc tggcgattgt cagcaatctt ggctttttaa cgcctatcga
    29161 tccgttgctc ggtaaattta tcgccgccgg attaattatc gcctttatgt tgctgcacct
    29221 gcgctccgtt gaaggcggcg cagcgtttca gacgctaatt accatcgcca aaattatccc
    29281 gttcactatc gtcattggtc ttgggatctt ctggtttaaa gcggagaatt ttgcctcccc
    29341 taccaccact gcgattggcg caacgggcag ctttatggcg ctgctggcgg ggatctctgc
    29401 caccagttgg tcgtataccg gcatggcctc tatctgttat atgaccggcg aaattaaaaa
    29461 ccccggcaaa accatgccac gagcgctgat tggctcctgt ctgctggttc tggtgctcta
    29521 caccctgctg gcgttggtga tttccggcct gatgcccttc gacaaactcg ccaactctga
    29581 aacgccgatt tccgacgccc tgacctggat cccggcactc ggcagcaccg ccgggatctt
    29641 tgttgccatc acggcgatga tcgtcattct tggttcgctt tccagctgcg tgatgtacca
    29701 gccgcggctg gaatacgcga tggcgaaaga caacctgttc ttcaaatgct tcggccatgt
    29761 gcatccgaaa tacaacacgc cgaatgtctc catcatcctg caaggggcgc tggggatctt
    29821 cttcatcttc gtttccgatc tcaccagcct gctgggttat ttcaccctgg tgatgtgttt
    29881 caaaaatacc ctcaccttcg gctccatcat ctggtgtcgt aaacgcgacg attacaaacc
    29941 gctgtggcgt actccggctt tcgggctgat gaccaccctc gccattgcat caagcctcat
    30001 tctggtcgcc tcaacctttg tctgggcacc gattcccggc cttatctgcg ccgtcatcgt
    30061 tattgccact ggtctgcctg cttacgcctt ctgggcgaag cgtagccgcc agctcaacgc
    30121 tttgtcgtaa tgttatctgg agaaaataaa atgttggata ttgataaaag caccgtggac
    30181 tttctggtca ccgaaaatat ggttcaggaa gtggaaaaag ttctcagcca tgacgttccg
    30241 ctggtgcacg ccatcgtgga agagatggtg aagcgcgaca ttgatcgtat ttatttcgtt
    30301 gcctgcggct cgccactcaa cgcggcgcaa acggcgaaac atctggcgga tcgcttttcc
    30361 gatcttcagg tctacgccat ttccggctgg gagttctgcg ataacacccc gtatcgcctc
    30421 gacgatcgtt gcgcggtgat tggcgtttct gactacggta aaacggaaga ggtaatcaaa
    30481 gcgctggaac tgggtcgggc ctgcggcgcg cttactgcgg cattcaccaa acgcgcggac
    30541 agcccgatta cctcggcggc ggaatttagc attgattatc aggccgactg tatctgggaa
    30601 attcacctgc tgctctgcta cagcgtggtg ctggagatga tcacccgcct cgcgccgaac
    30661 gcggaaatcg gcaagatcaa aaacgatctc aagctgttgc cgaatgcgct cggtcatctg
    30721 gtacgcacct gggaagaaaa aggccgccag cttggtgaac tggccagcca gtggccgatg
    30781 atttataccg ttgctgcggg tccgctgcgt ccgctgggtt acaaagaagg cattgtaacg
    30841 ctgatggaat ttacctggac gcacggctgc gtgattgaga gcggagagtt ccgccatggc
    30901 ccgctggaga ttgtcgaacc ggacgttccg ttcctgttcc tgctcggcaa tgatgaaagt
    30961 cgccacacca ccgaacgcgc cattaacttt gttaaacagc gtactgacaa cgtgatcgtc
    31021 atcgattaca ccgaaatttc gcaagggctg cacccgtggc tggcaccgtt cctgatgttc
    31081 gtgccgatgg agtggctctg ctactacctg tctatttaca aagatcacaa cccggatgaa
    31141 cgccgctatt acggtggtct ggtggaatat taatccctct cccggcccgg caacgggccg
    31201 gatttacgca aggagttacc cgatgaaaac aggtatgttt acctgcggcc accagcggct
    31261 gcctattgaa cacgcattcc gtgatgcaag cgagctgggt tacgacggca tcgaaatttg
    31321 gggcggtcgc ccgcacgcgt tcgcgccgga cttaaaagcg ggcggcatca aacaaatcaa
    31381 ggcgctggcg cagacgtatc agatgccgat tatcggctat acgccagaaa ccaacggcta
    31441 tccgtataac atgatgctgg gcgatgaaca tatgcgtcgc gaaagcctcg acatgatcaa
    31501 gctggcgatg gatatggcaa aagagatgaa cgcgggttat atgctgattt ccgcggccca
    31561 cgcgggctat ctcacgccac ctaatgttat ctggggacgg ctggcagaga acctaagcga
    31621 actgtgtgag tacgcggaaa acatcggcat ggatctgatc ctcgaaccgt taacgccgta
    31681 tgaatcgaac gtcgtgtgta atgctaatga tgtgcttcat gcgctggcgc tggtgccttc
    31741 gccgcgcctg ttcagcatgg tcgacatttg cgcgccgtat gtccaggcgg aaccggtgat
    31801 gagttatttc gacaaactgg gcgataaatt acgtcatctg catattgtcg acagcgacgg
    31861 ggccagcgac acgcattaca ttcctggaga aggcaaaatg ccgctgcggg aactgatgcg
    31921 cgatattatt gatcggggct atgagggtta ctgtacggtg gagctggtga cgatgtatat
    31981 gaacgagccc agactctatg cccgccaggc gctggaacgc tttcgcgcgc tgctgccgga
    32041 ggatgagaga tgaaacccct ggcgacaatc ggcgataact gcgtcgatat ctacccgcaa
    32101 ctgaataaag cgttttctgg cggtaatgcg gtcaatgtgg cggtgtactg cactcgctac
    32161 ggcatacagc cgggatgcat tacctgggtg ggtgacgatg actacggcac aaagctgaag
    32221 caggatctcg cccgcatggg cgtcgatatc agccatgtcc atacgaaaca cggcgttacc
    32281 gcacaaactc aggtggaact gcacgacaat gatcgcgttt ttggcgacta caccgaaggc
    32341 gtgatggccg actttgccct gagtgaagag gattacgcct ggctggcgca gtatgacatt
    32401 gtgcacgcgg caatctgggg acatgcggaa gacgcattcc cacagctgca cgctgcgggc
    32461 aaacttaccg ctttcgactt ctccgacaag tgggacagcc cgctctggca gacactggtg
    32521 ccgcatctcg attttgcctt tgcctccgca ccgcaagaag acgaagcgct gcgtctgaag
    32581 atgaaagcga ttgttgcccg tggcgcagga acagtgattg tcacgctggg tgaaaacggc
    32641 agcattgcct gggatggcgc gcagttctgg cgtcaggctc ctgaaccggt gacggttatc
    32701 gacaccatgg gtgccggaga ttcgttcatt gccggattcc tttgcggctg gtctgcgggg
    32761 atgacattac cgcaggcgat ggcgcaggga acggcgtgcg cggcacaaac tattcagtac
    32821 cacggtgcct ggtaggcata acgttggcgt gagcatcttc acgccaacgt gctgttactt
    32881 gccggaaaac gaccctataa tccgtgtaat tcattcttta tttcagggtc gattatgtca
    32941 gctacggacc gctactctca tcaactcctc tacgctaccg tccgccagcg gttgctggat
    33001 gatatcgcgc agggggttta ccaggccggg caacagatcc ctaccgaaaa cgagctttgt
    33061 acgcaatata acgtcagccg cattactatt cgcaaagcta tcagcgactt agtggcagac
    33121 ggcgtactga tccgctggca gggcaaaggc acctttgtac aaagccagaa agtagaaaac
    33181 gccctgctta ctgtcagtgg ttttaccgat tttggcgtct cacaaggcaa ggcgacgaaa
    33241 gagaaagtga tcgagcagga acggatcagc gccgcgccgt tttgcgaaaa gctgaacatc
    33301 cccggtaaca gcgaagtgtt ccatctctgc cgggtgatgt atctcgataa agagccgctg
    33361 tttattgata gttcgtggat cccgctgtcg cgttatcctg actttgatga gatttacgtc
    33421 gaaggaagct ccacctatca gttatttcag gagcgtttcg acacgcgagt ggtcagcgac
    33481 aaaaagacca tcgatatctt tgccgccacc cgcccgcagg caaaatggct gaaatgcgaa
    33541 ctgggcgaac cgttgtttcg catcagcaaa atcgcctttg atcagaatga taaaccggtg
    33601 cacgtctccg aactcttctg ccgcgccaat cgcatcacct taacgattga taataaaaga
    33661 cattaaccgt aggccggata agatgcgcca gcatcgcatc cggcgatgct ggcgcactga
    33721 attttacatc ccgcacattc ccctcaccct aaccctctcc ccagaggggc gaggggaccg
    33781 ttcagtacgg aatttagctt gacggtgcta tccagcatta aatactggca atactctcac
    33841 gcaaaatccg cagcaccgtc tcttcaccgc tgcgattcgg gttaatgcga atcgcgcaat
    33901 gttcgagctg tggattcgcc tggcgaaacg tcccggaaag tcgataaaaa agtggcggga
    33961 tttcatattt cgactctgca ccgaccgggt aaggcaacgc gccacgcttt tgcgcttctt
    34021 ccagcaccct ggcggcaatc ggctgatgaa actcgacaat caacaccttc gactgcgcat
    34081 tggcaatgac ggcgcttttc acttccgcca ccgcgccgcc gttaagcaaa gccagcaacc
    34141 gttcggatac tcccgcctgc accgcatgca tcactggcgc gaacaccaga ccacgcaaga
    34201 cttccagcgc ctgtgagccc tggatctggc taccgccgga gtaaagcgtg gcgcgaatcc
    34261 gactaataac atcggcatcg ccaaccactg caccaacgcc ctccggccca aacagcttga
    34321 agcaggagaa tgtcgagaca ttcgcgccgc attcacagcc gattcgcgct accttcatca
    34381 ccgcatagtt gtcatcggtt aaggctggaa cacttgccgc gcgcaacgtt gccagcacat
    34441 ctgccagcac gtagctgtcc tgcggctgct ggcgcgtatg ctgcaccagc gccgcatccg
    34501 gttgttgctc gtcgacgacc tgcttcagtg ccgacaggtc attgaaatca acagtaataa
    34561 gcgtcagccc catctgctca ataataaccc gtgtcgtcgg gtaaacaggc gcgtcatgca
    34621 ccagaagacg ctgccccggt ttgagcaaag ccgccagccc ggcgcgaatc gcgccagtcc
    34681 ccgcgccctg caccagcgcc gcagcctgtg cgtgaaatgc atcagccagc acctgctcca
    34741 cccgttgggt aatacacggt tgattcagcc ccggcgttaa gcccaaatca ccgctggtta
    34801 gaaactccgc gccgggaaag tgacggcaaa tcgtgtccac cagcgcaaac tgcttttgct
    34861 gcgcctcaat aagcgtcagg ctttgcagag gaaacgtctt cataggtgcc ttccttaagc
    34921 cggaacgaac aggccaagcc agtaaagaac attcagcaga ataccggtga tcattactgc
    34981 cacgaccggt gccgccattt tctgtaccgg acggcccagg gattcgttga ggaagtaaat
    35041 ggcaaccgcg atagagaatc cggtataacc cgccatctta attgctgcga aaatcgaacc
    35101 gaccagcagc gccacttcca tcagcatatt catggcgtta cggatgttat ccgacgcatt
    35161 acgcaccgat gggtagcgtc ccagccattt gccgatcgaa cgaagcaata agacttccgc
    35221 cgaaataacc actgcgccta ataccgctgc aaccatcgga ttcggcgaga gatagcccac
    35281 cgcataaaca aaggtaaagc ccgcaactgc atatacgccg gtggctaacg cggtggtggc
    35341 aatcaacggc acaaaaccca gtccgcgcat aaattccgcc agagcagcct ggttgatcag
    35401 cgtttgtgat tgttccggcg tcacgcctgc ggaatatgct ttctccagag tgaagatcga
    35461 cacttcacta ccggcgaaaa tcttcatgct ggcaacggcg gcaatcaatg ctcccacgat
    35521 ggcgatatac ggtaagtttt tgataatccg cgacgtacgt tcttcgaaca ccgaaagccc
    35581 gctggcatca atgtcatttt catcacgatg acgcaggtcg tgagttatgg cgatccccag
    35641 cagcatcacc atgccaataa agatttcgat ggattccgga ttgagatgcg ggaaatagcg
    35701 cacgactacc acacgggtca tcagtaccac cacggcagcg atcagacttt gcttccagcc
    35761 aaactggtag aaaattgcca ccagcgggaa cagtgcaaaa gcagaaacca ccggcgagct
    35821 taattccccg aggctaccta atacatccac cggcagcgcg gtcagcagct ggtttactgg
    35881 caacagacaa gtaaggatca acacgcccca gatagcgcca agaccaaacg ccatcaggct
    35941 gtttatcgcc agtacgccga ggatatcggt aggaagaaag agtaaccatg cgtttagcag
    36001 cccggttttc agggtaaaag agatccccac cgaggccaca aaaccaatgc tcagaccaaa
    36061 agcgatactc cccgcctcgc gacggttcat atagccttca atcagttgcg gcaggatcgg
    36121 gcggatgccg tcatgaaaaa cagccgccga gcgatgcgcc agaagcgatg tcatacccgt
    36181 caggcacgcc accacgataa tctgaatata cagatccata gtcgtgcctt ttatttcaga
    36241 tggttaatca acatcggaat cgcgtgttcg acgtgctcaa cggaaaggcc aaacgccact
    36301 tttccttcgg cgatcatttt ggcgatatgc tcgtctttcg ctttaatgcc tggtttggca
    36361 atggtgcaac ttttgttata gccaatcacc gcgatggcaa tcgacaatgc cgcgcccgca
    36421 ccggtattac acgcgccgat gtaataatcc agttgcccgg atttcacctt cattgccgct
    36481 tccatgtcgt tgtgaatgaa aacttcaaaa cagccaggag ccgtcgcttc aatagttttt
    36541 ttaatctgct cacgctgtaa gcctgcaacg ccaatctttt tcatgagtct gtccttattg
    36601 gaaaaattga gagggatttt cacgtaacat cacatccaca tcggcctgac tgaatcctga
    36661 ctggcgcaat tgcggaataa aggtggtcaa taaatagtca tagccataac caccattggc
    36721 ttttaaatgg gagcggcgcg taatatccat cgacagcatg acgcggttca gcaacccacg
    36781 gtcacgtagc gcgtggagca tcgcaatgtg cttttcgtcc gggtagtaac tgttcttacc
    36841 aatggtgtcg aactgcacgt acgcgccgag atcgatcatc ttcaaaatgt tgtcgaggtt
    36901 gtctttcaga tcgcagtgac caacggtgac gcgcgaaaga tcaactccgt gggcttgcaa
    36961 cagagccagt tgttccagcc ccatggtgct gaacgacgta tgcgtggaga tcgggcgtcc
    37021 ggtctggtta tgcgccagcg cagcggcaat aaataccttc tcttccagcg gcgtaatctt
    37081 tccttcgctg gagccgatct ccgcgatgat cccggctttc agctccgtgc catcgatacc
    37141 ctgttcaatt tcatcgacca tctcctgcgc cagttcctgc acgctgcggg tcaccacatg
    37201 ttccgggaaa aacgcatcct ggtaataacc agtacaggcc accacgttga tcccggtttc
    37261 gcgcattaca tcgagcataa attgcgcatt gcgccccatg taacagttgg tcatctcaat
    37321 cacattacgt acaccccggg tcatcaggtc gttcatctcc tggcaaatga acgcatactg
    37381 atcaaggcgg cagtccacgt tgtttttaaa gccggagaga tcaatatgca gatgctcatg
    37441 ggccagggtg taacccgtcg gatcaaaact catagggtgt ctcctgcaaa ccggagggag
    37501 gaaagaaaag agcgaccatt ttgtggcggt ggcgcacgga aaaattcaca cacggtagct
    37561 cccacatcag aaagcgtggt gcgcacaccg agctgcgtgg cgaccagccc ttgctgataa
    37621 accagcactg gcaccacttc acgggtatgg tggctgtgac caatggtcgg atcgttaccg
    37681 tgatccgcca tcacgaccag gcaatcatct ggctgcatcg cctcaacaag ccgggcaagg
    37741 ttacggtcaa cgacctgcaa acgttcggca taacgtgcga cgtcttctgc atgaccagcg
    37801 aggtcggttt cctggatatt ggtgcaaata aacgccgtcg gatgcgtgtt aaattcgtcg
    37861 agggtgatat ccataatccg ctggctatcc accaggtttt gccagctcac gccataagga
    37921 ttgctgacga tatctgccac cttacccacc agcacggttg gcacgcccgc ctcatacagt
    37981 ttttgcggca cctgcacttt ttcatcaaca ccgtagccca tatgcacgac ctggaaaccg
    38041 ttgtcataag cgccagaacg cggcgcattg atgccaataa agcgcccttc tttgctttct
    38101 gcggcatcga gaatacgctg actgtcggtt aacaagccgc caaatgtaat gacccgaccg
    38161 acctgtacct gctcacgcac gatacgacca attttgattg cgtcgtcaaa agagatcaca
    38221 gagagattgg cggtaatgtt atagacctgg cctaaatccg cctcgagatt atcgccaatc
    38281 gcaaccgcct gattgaccca cagaaattgc agatcatcgc cacggcgctc cacctgccag
    38341 ccagcggaaa ctaatgcctg ctcaacatgg tcaatcacat cgcgaaaagg catccgcagc
    38401 ggcggtaacg ggcgcgtgcc taaaatttcc tgatgcccca taaaggtatc gccaccttca
    38461 tgttgcagct ctgccacgcc ccaggttgcg gaatctgacg gctgcatatc gcctggcgca
    38521 taacccaatg cgttgattag ccccagcttc tccagcgttg gtagctgcaa atgcggcaac
    38581 tggctcagga tgtgaccaca ggtattcgct cccgcatctt gcggacgcac cagcgtgaca
    38641 tctttcattg cccctacgcc aaagctatca atcactaaca ccacaaatcg cgccattacg
    38701 cccccagaga gtttccaaga ctgtcataac gaccaacgat tttcggttcg ccacggtgaa
    38761 taccggacac cagcaccaca tcgctacggg tgacaaaaat ctgcgtgcgg aaacagagca
    38821 ccactgcact gcttaccgga aactcgcctg ccagcgacag ggtgtagtcg atactgctgt
    38881 catccacagt tttgagattg gtttcagtaa tcttttgatt ttctggcgta aacaccaacg
    38941 catgttgcgc atgaccacga cggtagtaac cgccgccgta gcagtagctg tcgccacgga
    39001 aatgatggga gatttcactc aaccagagca tcgcgatacg ttcagactga tcgccctgct
    39061 ggtttgccgg aatagtgccc gtcagcgcat gaccgggttc ggcatgagtc acaccgtatt
    39121 gcgccagtaa tggcagcgaa gtgcagctgg tcgctgaagg cgcgttcagt tgctcaatcg
    39181 caataccaga tttcgccagt tgatcccgtg cttgtaccag cgtgtgaaga ttcggtgtcg
    39241 gcaaaacttt cccggcagcc tcatcccaaa gcaggcaagg gaaatgggta agtccggcta
    39301 aatgcagccc cggcagtttc ttaatttcag caaccacctc gtgcagcgaa tgctgtacaa
    39361 aaccgctctc ctgccccgga taaagaaaat catcgtcgct ataaactttg agcagcacag
    39421 actggattcg cccggccttc accgccgccg cagaaacttc ccgcgctttg tcgagagtaa
    39481 acacggtgat gacgtcggtg ccctgttcaa cggcgtcagc aacctgatga caagggattt
    39541 gtaccagatg cccctgatgc gccaccggca aatcagcgcg gcgcatgact cgcgcctctt
    39601 tgtaatccac cgccacaatg ccgctgtagc ccaacgccag cagtttttcc gccagccatg
    39661 ggttacgacc gaattgtttg gtcatcagat acagttcaat gccgtaaagc cgcgccgtct
    39721 caatcagccg cttaccgttt tccagtatct gatccacgtc gatcacccag ctgtccgggg
    39781 cgattttgcc ctgctgccac aaacttagtg cggcggaaat cagcgccggg ttctggcgtt
    39841 tcaatgcttc tacaaacatc ttgttgcctc gtttctgttc cttgaattaa atattcattt
    39901 ttttgaatat ttaggttgtg cgacgtggat ttgtcgggag cttcaaacct cttcgttagc
    39961 agccatccat aatccataaa ggttggccag caaatagcct tcttcacacg ggttaacttc
    40021 cagtgcgaat tccttcaaca acacctgatg caattgcaca acggcttgcc agtggctgga
    40081 ttgcgccagt tctgccagca actcgttatc cagcggctct atttcttcac cacggcgact
    40141 gcgcatcagt gcactcgcca tatgtgtcat cgccatcgtt ccttgctcac tgcgcaccgg
    40201 cagatggcac tctgtttcca gtacgttgac gacctgcatc atgcctttgc agatgtcctt
    40261 atcaataacg cctgcctcgc aaagcaggtt gagtctggtt tccatatcag atgcctttcg
    40321 tttaataact cggttcctgt tccccactca ctacccgttg ttgatgggcg agcagggagt
    40381 gtttatctac aacggcacgc agcagttgtt gcattgggta atcatcgact cgcgtcagga
    40441 ccacggcttc ggtggcctgt aaaaatcgcg gatcgtctgt gagcggcgtc gcctctaatc
    40501 ccagcatggt cagttcgttt tccgccacca cgttccagat caccgcatcg acatcgcctt
    40561 taacaatgcg ttgtaaactc tcgtgataag agagatcgac acgttccaca tcactatcgc
    40621 caaaaaagac atcggtcatg attttctgat ccgccgaacg gttatccagc cccacgcgct
    40681 tcacgtttgc ggactcgcct ttacggcaaa tcaactggtg ctcgccaacg taggtgtgcg
    40741 gccccaactc cagcgcgagg cataaccctt tttgcgtgag ataactttcc gccgccagtc
    40801 gggaaaccac cgccatgtca tacacgccgt taagtaaaca ctccacgcga atatccgcgc
    40861 cgcgcatatg cgcatagtaa aaaggaatgc catcaaactg agctttcagc ccgctcgcta
    40921 aaccttcgta caagcgggta tagggcaaag gcatcgcaca taccacgttg ttgatatcca
    40981 catgactcag caatgctttg ttatccatct cgaccagata actgccattg cgcccacggc
    41041 gttcaatccg tatcgctccg cttgattcca gcgttttcaa cgcggcctgc gtcaggccaa
    41101 ccgatgaacg gcattcagtt gccagctcat ctatggtttt caacctatta ccgcattttt
    41161 cacccaacaa ataacgggcc agcgtcgtga taacgacgcc ttcttttttg ataaacgttc
    41221 gacgcataat atattttcaa taaaatgaat gtttatatct tcattaaaat gaagataagt
    41281 ggcaactgtg agatgtggaa acggcgaggc gcttcacatt ttttcggatt attgcaaatg
    41341 aggataaaaa aaccgggttt ccccggtttc agagtgatga cgaagtgatt tattcgacaa
    41401 tgcgagcaaa gggtaattcc cactggcgct tgcctgctcc cctcacccta accctctccc
    41461 caaaggggcg aggggaccgt ccgagcgttt tgatattacg gcttcgccac aaaaccaatc
    41521 gcttcgtaca ccgctttcag cgtacgggaa gcgtgtacac tggctttttc tgcgccgtct
    41581 ttcatcacct gttgcaggaa ggcttcatcg ttgcggaaac ggtgatagcg ttcctgcaat
    41641 tcagtcagca taccggaaac ggcatcagcc acttcacctt tcagatgacc atacatcttg
    41701 ccttcgaact gtttttccag ttctgggatg ctctggcccg ttaccgcaga aaggatatcc
    41761 agcaggttgg aaacgcccgc tttgttctgc acatcgtagc gaactaccgg cggctcgtca
    41821 gaatcagtga ccgcgcgttt gattttcttc actaccgatt tcggatcttc cagcaggccg
    41881 ataacgttat tgcggttatc gtcagacttg gacatcttct tggtcggctc cagcagcgac
    41941 attacgcgcg cgccagattt cggaataaac ggctccggca ccttaaagat ctcgccatac
    42001 agcgcgttga aacgctgagc gatatcgcgg ctcagttcca ggtgctgttt ctggtcttca
    42061 cccaccggta ccagattagt ttgatacagc aggatgtccg cagccatcag caccggatag
    42121 tcaaacagac cagcgttgat gttctcggca taacgcgcag atttatcttt aaactgcgtc
    42181 atgcggctca gttcgccgaa gtaggtatag cagttcagtg cccagcctaa ctgcgcatgt
    42241 tctggtacgt gggactgaac aaaaatggtg cttttctcag gatcgatacc acaagccaga
    42301 tacaaggcca gcgtatccaa cgtcgcttta cgcagcttct gtgcatcctg gcgcacggtg
    42361 atcgcgtgtt ggtcaacgat acagtaaatg caatggtagt catcctgcat gtttacccac
    42421 tgacgcagcg cacccatgta gttaccaatg gtcaattcac ctgagggctg tgcgccacta
    42481 aaaacgatgg gcttagtcat ttttcgattc ctgattttcg ctatgcggaa gccctaatgc
    42541 gggcagaagg tcatttatag actgataaat tacatcaggc tggctgagat cgatagcctc
    42601 gccgtagtta tatccgtagg ttaagccaac tgatgggcaa cctgccgctt ttgccgcctg
    42661 aatatcattg cgtgagtcgc cgacaaacag catctgttgt ggggcaattc ccatccgctc
    42721 agccaccagt aacagcgggt ccggatgcgg ttttttgttt tgcacatcat cgccgccgat
    42781 aaccacgcta aagtatttgg cgatatctaa ggcttcgagc agcggcgcga cgaacggcgt
    42841 cggtttgttg gtgaccaggc ctagcggcag gcctttagcc tgcaacgcgc ccaacgtatc
    42901 ggcaacgtgc gggaacaaaa acgtcccctc ttcggcaacc tcgccatagt agcgatcgaa
    42961 cagtttacgc agaatacgta cctgttcttc tgccggaatg tcgtcatcaa cgggcggttt
    43021 acccattgtt ttacgcagag tcgcacgttc ctgacgcgcc caggtcaatg cgcgctccat
    43081 cagaacatct gcgccgttac caatccaggt aataacacgt tcttcacctg caacgggcaa
    43141 ctccagcgca tacagcgcca tatctaccgc agcagcaaga ccgggagcac tgtccaccag
    43201 cgtaccgtcg agatcaaaag cgacgccgcg aatatcttca aacttactca tgacttacct
    43261 ttgccagttc actgcgcatt tcatcaatga cttttttgta gtctggctgg tcgaagattg
    43321 ccgaaccggc aacgaacata tccgcgcccg ccgcggcgat ttcgccaatg ttgttcacct
    43381 tcacgccacc gtccacttcc agacgaatgt caaagccaga ctcgtcgata cgacgacgta
    43441 cttcgcgcag tttatccagt gtttgaggaa tgaaagactg accgccgaaa ccaggattga
    43501 cggacatcag caggatcaca tccagcttat ccatcacgta atccagatag ctcagaggtg
    43561 tcgccgggtt aaataccaga cccgctttac agccattttc tttaatcagt tgcagcgtgc
    43621 ggtcaacatg ctcggaggct tctggatgaa aggtaatgat gctggcacca gcggcagcga
    43681 aatcaggcac aatgcgatcg acaggtttca ccatcaggtg tacgtcgata ggggcggtaa
    43741 tgccatagtt acgcaaggat ttcagcacca ttggcccaat cgtcagattg ggaacatagt
    43801 ggttatccat gacgtcaaaa tgcacgacat cagcgccagc tgccagggct tttgcggtat
    43861 cttcacccag gcgggcaaaa tccgccgaca gaattgaggg ggcaatcaaa tactgtttca
    43921 tccgcttctc cttgagaatt attttttcgc gggtgaaacg actcctggtt tgtacaaagc
    43981 cagcagttcg tccacctttt tacgtgtgcc gccgttgctg cttatactgc gtcgaacttt
    44041 gacgacatgc aattttgcgc gctgatacca ctcacgcgtt aacatcgtat cgtgattgga
    44101 gatcagcact ggaatatggc gatcaaccag accttcggcg atctccgcca gatgcgcttg
    44161 ttgttcaagc gtaaaactgt ttgtgtgata cgccgtaaag ttggcggtcg cagatagcgg
    44221 tgcataaggc ggatcgcaat agacgacgga tgcatcatct gcgcgcgcca tgctatcggc
    44281 gtaagactca caatagaaaa aggcattctg cgctttttca gcgaagtgat acaactctgc
    44341 ttccgggaaa tagggttttt tgtagcggcc aaacggcacg ttaaactcac cgcgcagatt
    44401 gtaacgacac aggccgttgt aaccgtagcg gttcaaatat aaaaacagta ccgcccgacg
    44461 gaacggatcc tggcttttgt tgaactcttc gcggaactga tagtaaacct cggcgcaatt
    44521 tgtttcggga acaaacagct cgcgtgcggc ctgtacgtac tcatcagtac gcatcttcac
    44581 aatgttatag agactgatca ggtcgctatt gatatcggca aggatataac gagaaaagtc
    44641 ggtgttgaga aacaccgacc cggcacctac aaaaggctca accagacatt cgcccttggg
    44701 caaatgccgt ttaatatcat caagcagggg atacttgccc cctgcccact tcaaaaaagc
    44761 gcgatttttc ttcatgctga ctaactaatt acaccttctc cggctgtgga gaaagctccg
    44821 acagcatcca gcgcttcagg caactcccgc aacgataaac cattgcggga gataaccttg
    44881 attacttcag atcggcctgt acctgacgca gcggtttcgc ccacgggttt ttggcctgga
    44941 catctgctgg caatgtagat accgcttttt tcgcctcttc tttcgaagcg tacacgccag
    45001 aaaccaggac ataccacggc tgaccattac gcgtcgtttc atagacaacg tagtttttca
    45061 gattctcttt cttcgcccaa ccgttcaggt tgtcgtagtt agaggaactg ctcagctgca
    45121 gagtgtaatg gctggacggt gccgatttca acgaacccac attacctgcg gtcttccctc
    45181 cagcggctgg tgttgccgtg gtttgcgccg gggatgccgt ctgtactgga gccgtagtcg
    45241 ccgtctcttt tggtgctggc gtagaagtcg cagccggtgc cttcgtgctc gccacaggag
    45301 cagctggttc agtacgcttc ggcgtctgtg ctaccggctt cggttccgtt ttcacggttg
    45361 cttgcggttt tttcggttca atcaccgcct gctgacgagc tggacgcgta gtggccggac
    45421 gttcagcagt ttgcgttttc gccgtgtcac gcgatgcatt gccattgcgt actggcgcga
    45481 cagttgctgg ttcagtcggc aatgtagaat tgaccgccac attgttcaac tgttgctgat
    45541 tttgtggctg ggtcagcgca ttgttcaggt caccctgcac ttcaacacgt tgttgaccat
    45601 ccgttgccgc cggggtttgc ccttgagtcg gcgtagaaga gatcggtggc agagaaacat
    45661 cctgctgagt attttccgca gacgtggttc ccggcgctgg ctgcacccca ttcgcctgat
    45721 cggtcgcatt gcctgcaaga tcaatactct tctcgccaga cgcggtttga tcgctggaag
    45781 aggtcgaggg ggcttttagc gcagaaccga taccgatgat caacagcagt agaaccagaa
    45841 tgccgacgcc catcatcata tactgacgag aagcgggttt actggctgct tttttgcgct
    45901 tacgcggacg acgctctacg cgctcttcat ccacggtttc atcttcggat tcgtcaattt
    45961 cttcttcgat ttccggttcc tcattgcgct ctttttgcgc acgagtcgga cggcgatcgt
    46021 cagtgtcatc aagttcaata tcatcaaaat tgatctgcgg ttcgccacgt tcagtacgct
    46081 cagaacgttc agaagattga cgagaacgac cagtacgacg atcgctggga tcgggtttca
    46141 gctcgtcttc tggtttgaat tcatccattt aacaccccac taaaaggtta atgcttacca
    46201 cgttgcaatt aacctgaagc taatagaccg cttgataagc ggcctgacct ttcttgttgt
    46261 tacgctgatt gacaatcggc aatggcgtta agaacaagct cgtgcgaaac gccgctgcga
    46321 acttcactct taccaattgc caacggaaga attaagcgca tctctcccgc aaggactttc
    46381 ttgtcacgca gcatatgcgg taaatacgcc tgcgcggaca tttcgcgcgg cccattgacc
    46441 ggtaacccag cccgcgtgag cagggttata atacgctgcg tttcggcaga actaaactgc
    46501 ccgagacgtt ccgacgtccg cgccgccatc accatacccg cagcgaccgc ttcaccatgt
    46561 aaccaattgc cataccccat ttcagcttca atggcatgac caaaggtgtg tcccagattc
    46621 agtaaagcac gtaacccggt ttcgcgctcg tcggcggcga caacttctgc cttcagttca
    46681 caacaacggc gaatacagta cgccattgcc ggaccgtcca gacgcaacaa cgcatccaga
    46741 ttctcttcca gccagttaaa aaacgcaccg tcaagaataa tgccgtattt gatgacttct
    46801 gccagccccg acgctaactc acgcggggga agcgttttca gacagtcgag atccaccacc
    46861 actgaagcag gctggtagaa cgcgccaatc atgtttttac cgaggggatg gttgaccgca
    46921 gttttgccgc caacggagga atcgacctgc gacagtaacg tcgtcgggac ttgaatgaaa
    46981 cgaacaccgc gctgataact cgccgccgcg aagccggtca gatcgcccac tacgccgccg
    47041 ccaagcgcca ccagcgtagt atcgcgacca tgcggctttt gtaacaacgc cgtaaagacg
    47101 gtatcgagta cagccaggct tttatactgc tcgccgtcag ggaggataac gctatcgacg
    47161 ttaacacccg cctgttcaag tacgccgcgg accttatcga gatacagagg agccagggtt
    47221 tcgttggtga ccaacatgac ctgctcgccc gatttcagcg gtaagaatga agctggttca
    47281 ttaaacaaac cagatgcgat ggtaattggg taactacgtt ccccgagagt aacgacaatc
    47341 ctctccataa cgcgacatcc accttaatta ctgtacccgc agacgagtgt atataaagcc
    47401 agaattagtt gctttccagc atgtgaataa tctggtttgc aaccacttta gcgctttgat
    47461 catcagtacg aatggtcacg tcggcaatct cttcatacag cggattgcgt tcattggcca
    47521 acgcttccag aacttcacgc ggcggtgttt caacgtgcag caacgggcgt tttttatcac
    47581 gctgcgtgcg tgcaagttgc ttttcgatgg tcgtttcaag ataaacgaca acgccacgag
    47641 cagaaagacg gttacgcgtt tcacgggatt tcacagagcc gccgccagta gccagcacaa
    47701 taccctgttt ctcggtcaac tcattgatga ccttttcttc gcgatcgcgg aagccttctt
    47761 cgccttctaa atcgaaaacc cagcccacat cagctccggt tcgtttctca atctcttgat
    47821 cggaatcgta aaattccata ttgagttgtt gagctaactg gcgcccaata gtgctttttc
    47881 cggcacccat aggcccaacc agaaagatat tgcgtttctc tgccattttt tcggtactac
    47941 taagactatt cgttaatgat aaacccgctt cgctcagaga gcgccgcagg acatgaactg
    48001 aaacctcata agatattgcg agagtcagac tgaaaattat ctcaatactc cagcgggttt
    48061 ggcaactgaa taaatcaccc atccctctgc atatctggtc gctgcaagcg cgctgccttg
    48121 ctaccaccgc tctggcgata aatcaccggg taagattagc gtaaaaaaga cagcaaaatg
    48181 ccgcctgaat gataaatcat catcatggaa cgcaggcagc gaagagcacc aactctcaaa
    48241 tcggtactcc ttgtatgcta aatacctgcg cgcgtcaaat agatgaaaca cgttcagcat
    48301 aaaaacatta ccgcttttac ggctgtttac tcactggaaa ccagtcgtgg cgtgataaac
    48361 accactaact cgcgtcgttc atcttctttt ccgtcatgac gaaataattg cccgaaccag
    48421 ggaatgtcgc caagcaacgg tacgctatcc tgacccgatt tatttttacg ggtaaaaatg
    48481 ccgcccagcg ccaacgtttc tccgcttttg acctcgacct gcgtttcgat ctcctgctta
    48541 tcaatcgcca gcacttcgcc atcggcctgc tgtagcacct gccccggaac gttctggctg
    48601 atgtgtaatt tcagccggat gcgacctttt tgtaacaccg tgggcgtgac ctccatcccc
    48661 aggacggcct ctttaaattc caccgacgtc gcgccacttt ccccgctgga aacctgatat
    48721 ggaatttcgc tcccctgttt aatgctggca ggctgaagat gtgaggccag cagacgcgga
    48781 ctggcgataa tatccagctg ctgtttttgt tcgagcgcag aaagctcaag atccagcaag
    48841 cgtccgttga tgcgcccaat gttaaaaccg acatgcgttg tcgccgtcgc tacggagagg
    48901 tcgctaccaa gcgtggtgac ttgcccaacg ccaccagcgt gttgcgcatc ggccagcgtc
    48961 catttcacgc ctaactcacg caaacttttt tcattaatgg tgacaatatg cgccgacagc
    49021 tcaacctgcc cgaccggcag atccatttgc gctacccact gttcaagcgc gcttaacgcc
    49081 gttttgttat ctcgtagcaa aaggcgattg gtgcgtttat cgacggtcat actccctttg
    49141 gcactcagta gcttctcccc cgctttcgcc agttctcccg cgtcggcgta ttgcagggtt
    49201 atactgcgat tttccagcgg cagatttgcc tgcgcccgcg cctgctccgc ctcctggcgg
    49261 gcgatattgt tattctgcca ggcaatggaa tgcactgaga gaatgttgtc ttcctgccgc
    49321 gttatcagtc cggcgctttt cactacagtt tgtagtgcct gcttccaggg aacatctgtt
    49381 agatgtaacg acaccgtacc gctgacgtct ggcgacacga ccaggttcaa cttctcctgt
    49441 tcagccagcg cctgcaacac ctgagctacc ggaacgtcat ccaccatcag cgtcactttt
    49501 tgcggctttg ccgcctgtac gccgggtatc agcatcaaca gtagtgcggc tatccattgc
    49561 ttcatttgta tctccttgcc gttgccacaa ccattgtggc ggttcgcagt ttgtcccggt
    49621 tcccagcgtt agtgcgtctg gcgtcagctg taaaattgtc cagccgtttt ccagcacatc
    49681 gttttgctgc acccgtcgcc atttcttttg cccgtctttt attacaccga tgatgtgctc
    49741 gcctcgccct accatcccct gatagcgcca ctggctaagt tcgctaatcc ggcatagatc
    49801 ttccggcggt ttaaaagggt cacgcatacc ggttaaaagg cacaatgcaa tacctgccaa
    49861 caaccagcgt ttaaccctca tgcggtgtct ccagttgtag cgtgaacaaa agatcatcac
    49921 cttccacgct taacgaaaaa cggctcacgc tgacgttgcg ctctgccagc cgtgtaaatg
    49981 ccgacggcac tgcttcccac agcgttttca acgccaactc acctccctgc gcggatggat
    50041 gccaggaaac cagttgcgcg ccggataact gaaaatccag aggcgaaaag ggcagcgttt
    50101 tttcctcgct gaagggagtg gtgtctacca ggcgatacag tgcggcccac tgctggtgat
    50161 gacttgcccg tagtcgaatt aatgcgtcgc gctcttcatg gtgtgtcgat gatagaaaaa
    50221 tgagcgtaac taacatcagc aaccagaatg cccagcaaag ctggcggagg cggggtgatg
    50281 tggcgaacca ccagtcaaag aacatgttca tcgctaacct tccttgttaa ctgatactca
    50341 aattgccagc gtccctgcgc atcctgctgc gtggctcccc gctgattgag atgaaaagac
    50401 gcatcctggc ggagtgacgt ttctagtgcg tttaacgcgg taatgcttgt tgtcagcccc
    50461 ttgatctcca gcgttccctg ctgccagctt atcgttgtca gccaggcgtg ctcgggtaaa
    50521 agcgccgcca gtgcttccag cgcagattgc cagtcgcggg taaattgtcg ctggcgctgc
    50581 cgttgcgaac gctgttcgcg taactgctgt tgctccagca aacgtggctt cgttatctgt
    50641 aagctgcggg cgagttgttg ttccgcctga agcaaaactg cgtctatgcg cgcttcggcg
    50701 ctgcctgtca gacgcagtat tagcgttatc ccgacggcca gcagcagagg cgcaacgaac
    50761 atcagcaacc agaaacgcag aaaagcggtc cggcgttgct gtcgccaggg caaaaaatta
    50821 attggcgggt tcatcagtac gccttcccaa gcgccagccc cagcgcgatg gcaaagtctc
    50881 cacctggcgg cggtagcggc ggctgacgaa cagaaacggc ctcccaggga tcaaatccgc
    50941 cttcgccaca tatcgcgacg ctttcgggat caacggataa cgctgccgcc agctccttcg
    51001 cgctagtcat ccccaccgcc agtttgcgcc cccagcgaca gcgtgtcgcc cacagccact
    51061 gttcgttatc acgccaggcc agacattgct gatgagaagg taaaaaaggc aggaattgct
    51121 gtaatgcact ggcatccggg gtgatcgcgc tcacatgaac acgcaacctt tctgccagag
    51181 taagtagcgt tgccagctct ttgctttgcg cggcagtcac gttataggcg gggctgagtg
    51241 agtcttcgct gtaatcgaag cgcagggagt ccggatccat atccagctcg cgggccatcg
    51301 tccctgacag ccaggccgtt tgctcccgct caccaaggga catcgacggg cgcggaaatg
    51361 accgctgtaa tgtgcgactg gcgggaaacg ccaacataat gtgatgacgc tgcggcagtt
    51421 cgcgactcca aggtaacaac gttttagcca gctgctgcgc atcaacaatc cgcccatctt
    51481 tgataatgtc gttctccagc ggcaaccgcc accagcgttg caaaaagcat tcttttgcgc
    51541 cccgcacgat cgcaaccgct accgcttctt gctgttgtaa atgcaaacca atttgccaga
    51601 tcttaaatgc cattgtgatg atctccttat cacccgtcac tctgacgggt atatcaatgc
    51661 gtctggcttg cctttatact accgcgcgtt tgtttataaa ctgcccaaat gaaactaaat
    51721 gggaaatttc cagtgaagtt cgtaaagtat tttttgatcc ttgcagtctg ttgcattctg
    51781 ctgggagcag gctcgattta tggcctatac cgctacatcg agccacaact gccggatgtg
    51841 gcgacattaa aagatgttcg cctgcaaatt ccgatgcaga tttacagcgc cgatggcgag
    51901 ctgattgctc aatacggtga gaaacgtcgt attccggtta cgttggatca aatcccaccg
    51961 gagatggtga aagcctttat cgcgacggaa gacagccgct tctacgagca tcacggcgtt
    52021 gacccggtgg ggatcttccg tgcggcaagt gtggcgctgt tctccggcca cgcgtcacaa
    52081 ggggcgagta ccattaccca gcagctggcg agaaacttct tcctcagtcc agaacgcacg
    52141 ctgatgcgta agattaagga agtcttcctc gcgattcgca ttgaacagtt gctgacgaaa
    52201 gacgagatcc tcgagcttta tctgaacaag atttacctcg gttaccgcgc ctatggtgtc
    52261 ggtgctgcgg cacaagtcta tttcggtaaa acggtcgacc aactgacgct gaacgaaatg
    52321 gctgtgatag ccgggctgcc gaaagcgcct tccaccttta atccgctcta ctcaatggat
    52381 cgtgccgtcg cgcggcgtaa catcgtactg tcgcggatgc tggatgaagg gtatatcacc
    52441 caacaacagt tcgatcagac acgcactgag gcgattaacg ctaactatca cgcgccggag
    52501 attgctttct ctgcgccgta cctgagcgaa atggtgcgcc aggagatgta taaccgttat
    52561 ggcgaaagtg cctatgaaga cggttatcgc atttacacca ccatcacccg caaagtgcag
    52621 caggccgcac agcaggcggt acgtaataac gtgctggact acgacatgcg ccacggctat
    52681 cgcggcccgg caaatgtgct gtggaaagtg ggcgagtcgg cgtgggataa caacaagatt
    52741 accgatacgc tgaaggcgct gccaacctat ggtccgctgc tgcctgccgc agtcaccagc
    52801 gccaatcctc agcaagcgac ggcgatgctg gcggacgggt cgaccgtcgc attgagtatg
    52861 gaaggcgttc gctgggcgcg tccttaccgt tcggatactc agcaaggacc gacgccgcgt
    52921 aaagtgaccg atgttctgca aacgggtcag caaatctggg ttcgtcaggt tggcgatgca
    52981 tggtggctgg cacaagtgcc ggaagtgaac tcggcgctgg tgtcgatcaa tccgcaaaac
    53041 ggtgccgtta tggcgctggt cggtggcttt gatttcaatc agagcaagtt taaccgcgcc
    53101 acccaggcac tgcgtcaggt gggttccaac atcaaaccgt tcctctacac cgcggcgatg
    53161 gataaaggtc tgacgctggc aagtatgttg aacgatgtgc caatttctcg ctgggatgca
    53221 ggtgccggtt ctgactggca gccgaagaac tcaccaccgc agtatgctgg tccaattcgc
    53281 ttacgtcagg ggctgggtca gtcgaaaaac gtggtgatgg tacgcgcaat gcgggcgatg
    53341 ggcgtcgact acgctgcaga atatctgcaa cgcttcggct tcccggcgca aaacattgtc
    53401 cacaccgaat cgctggcgct gggttcagcg tccttcaccc caatgcagct ggcgcgcggc
    53461 tacgcggtca tggcgaacgg tggcttcctg gtggacccgt ggtttatcag caaaattgaa
    53521 aacgatcagg gcggcgtgat tttcgaagcg aaaccgaaag tagcctgccc ggaatgcgat
    53581 attccggtga tttacggtga tacgcagaaa tcgaacgtgc tggaaaataa cgatgttgaa
    53641 gatgtcgcta tctcccgcga gcagcagaat gtttctgtac caatgccgca gctggagcag
    53701 gcaaatcagg cgttagtggc gaagactggc gcgcaggagt acgcaccgca cgtcatcaac
    53761 actccgctgg cattcctgat taagagtgct ttgaacacca atatctttgg tgaaccaggc
    53821 tggcagggta ccggctggcg tgcaggtcgt gatttgcagc gtcgcgatat cggcgggaaa
    53881 accggtacca ctaacagttc gaaagatgcg tggttctcgg gttacggtcc gggcgttgtg
    53941 acctcggtct ggattggctt tgatgatcac cgtcgtaatc tcggtcatac aacggcttcc
    54001 ggagcgatta aagatcagat ctcaggttac gaaggcggtg ccaagagtgc ccagcctgca
    54061 tgggacgctt atatgaaagc cgttcttgaa ggtgtgccgg agcagccgct gacgccgcct
    54121 ccgggtattg tgacggtgaa tatcgatcgc agcaccgggc agttagctaa tggtggcaac
    54181 agccgcgaag agtatttcat cgaaggtacg cagccgacac aacaggcggt gcacgaggtg
    54241 ggaacgacca ttatcgataa tggtgaggca caggaattgt tctgattaaa aaggcgcttc
    54301 ggcgcctttt cagttggctg acaaagtgca tttgtttatg ccggatgcgg gtgaacgcgt
    54361 tatccggcta acaaaatcgt gaaaaatcaa tatattgcag gaaccccata agcctgataa
    54421 gcgttgtgca tcaggcaaac ttcacgcatt tacactcgcc cctgcccttt caaccattcg
    54481 cgcacgagga acagcgcgct gacattacgc gcttcgttga agtcagggtc ttccagcaaa
    54541 tccatcatat gcgccagcgg ccagcgcacc tgtggtagcg gctctggctc atcgccttcc
    54601 agcgattccg ggtagagatc ttgcgcgacc acgatattca ttttgctgga aaagtaagac
    54661 ggtgccatgc tgagcttctt caaaaaagtc agatcgttcg caccaaatcc aacctcttct
    54721 tttagctcgc ggttagcggc ttcgtagacg ctttcacccg gatcaattaa tcctttcgaa
    54781 aaacctaatt cgtaggattc agttcccact gcgtattcgc ggatcaggat caggtgatcg
    54841 tccacaatcg gcacaatcat cactgcttcc cggttggttg gacgcattcg ttcataaaca
    54901 cgccgcacgc cattgctgaa ctccagatcc acgctctcga cggtaaacag tcgggaacgg
    54961 gctacagttt caacattcag aatggtgggt ttttgtaatg atttgctcat cgtgggatct
    55021 atgctgtgaa atcagcggtt attgtgcgat atcggacacg ctttcggcaa tgtgaattgc
    55081 atgttattta catttatgta acttaataaa taatcgtcct caaatcaaat taaaagtcaa
    55141 taggttgaaa taactccagg aatttgctga tattccgcct tcagagggtt tgctatgatc
    55201 agcggttact gtgatgtgct taatgatgct caagttaaac tccacgcttg ccgatagcca
    55261 accgcagaat catgtattgt gtctggtgcg actgaccacg cctgacagac taagtaagat
    55321 ggggaaagca tgagcaccat tgtgattttt ttagctgctt tgctggcctg ctcactactt
    55381 gcgggatggc tgataaaagt gcgatccaga cggcgtcagc tgccctggac caacgccttc
    55441 gcggatgcgc aaacgcgtaa actcacacct gaagaacgta gcgccgttga aaattatctt
    55501 gagagcctga cgcaggtatt acaggtgcct ggcccaacgg gagctagcgc ggcaccgatc
    55561 tctctggcgc tgaatgccga aagcaacaac gtcatgatgc tgacacacgc tatcacgcgt
    55621 tacggcatct ctaccgacga tccgaataaa tggcgttact acctcgattc ggtagaagtc
    55681 cacctgcccc ctttctggga acaatacatc aacgatgaga ataccgttga actgatttat
    55741 accgattcgc tgccgctggt tatttcactc aacggtcata cgctgcagga gtacatgcag
    55801 gaaacgcgcg gctatgcctt gcaacctgtt ccgtccacgc aggcgtcgat tcgcggggaa
    55861 gagagtgagc aaatcgagct actcaatatt cgcaaagaaa cgcatgaaga atatgcgctg
    55921 agtcgtccgc gcgggctgcg tgaagcgttg ctgatcgtcg cctccttcct gatgttcttt
    55981 ttctgcctga ttaccccgga tgtatttgtt ccgtggctgg caggcggcgc attactgctg
    56041 ctgggcgcag gtctgtgggg gctattcgcg cccccggcaa aatcctccct gcgagaaatt
    56101 cattgtctgc gcggtacacc ccgtcgttgg ggattgtttg gcgaaaacga tcaggaacag
    56161 atcaacaata tttcactcgg tattatcgac ctggtctatc ccgcacactg gcagccatac
    56221 attgctcagg atctcggtca acaaaccgat attgatatct atctcgaccg ccacgtagtg
    56281 cgtcaggggc gatatctttc gctgcatgat gaagtaaaaa actttccgtt acagcactgg
    56341 ctgcgcagta cgattatcgc tgcgggctcg ctgctggtgc tgtttatgct gttattctgg
    56401 atcccgctgg atatgccgct gaaattcact ctctcatgga tgaaaggcgc gcagaccatt
    56461 gaagccacca gcgtaaaaca actggctgac gctggcgtgc gggtaggcga tacattgcgt
    56521 attagcggta cgggaatgtg taatattcgc acttccggaa cctggagcgc gaaaaccaat
    56581 tcaccttttt taccgtttga ctgctcgcag atcatctgga atgacgcccg ctcattacca
    56641 ttaccagaat ctgaactggt caacaaagcg acggcattga ctgaagcagt taatcgccag
    56701 ctgcacccta aaccggaaga tgaatctcgc gtcagtgcct cattacgttc agcgattcaa
    56761 aaatccggca tggtattgct tgatgatttt ggcgacattg tactgaagac agcggattta
    56821 tgttctgcca aagatgactg tgtgcgactg aaaaatgcgc tggtcaatct cggcaacagt
    56881 aaagactggg acgcgctggt aaaacgcgcc aacgccggga agctcgatgg cgtgaatgtg
    56941 ttattacgcc cggtgagtgc agaatcgctg gataacctgg tggcaacctc caccgcgccg
    57001 tttatcacgc atgaaacggc gcgagcggca caatcactaa acagtccggc ccccggcgga
    57061 ttcctgattg tcagcgacga aggcagcgat tttgttgatc agccctggcc ttcggcatca
    57121 ctttacgact acccgccgca agaacagtgg aacgctttcc agaaactggc acaaatgctg
    57181 atgcatacgc cgtttaacgc cgaaggtatc gtcacaaaaa tcttcactga cgccaatggt
    57241 acgcagcata ttggccttca tccgatcccg gatcgttccg gcctgtggcg ctatctcagc
    57301 accacattgc tgctactgac gatgctgggt agcgccattt acaatggcgt acaggcctgg
    57361 cgtcgttacc agcgtcatcg cactcgcatg atggagattc aggcctatta tgaaagctgc
    57421 ctgaacccgc aactgatcac cccttcagaa agccttatcg aataacacgt ttgcgcggca
    57481 ggttatgcta ccctgtcgcg caaattgctt cactctggag atttccctca tgcatatcaa
    57541 cattgcctgg caggacgtag ataccgttct gctggatatg gacggcacgt tgctcgacct
    57601 cgccttcgat aactatttct ggcaaaagct ggtgcctgaa acatggggcg cgaaaaacgg
    57661 ggttacgcca caggaagcga tggaatatat gcgccagcaa tatcacgacg tacagcatac
    57721 gctaaactgg tactgtcttg attactggag tgagcaactg ggtctggata tctgtgcgat
    57781 gaccaccgag atgggaccgc gtgccgtact gcgtgaagat accattccgt ttcttgaggc
    57841 actgaaagcc agcggtaagc agcgaatttt gctcaccaat gcgcatccgc acaacctggc
    57901 ggtaaaactt gagcataccg gtctggacgc acaccttgat ttattacttt ccacccacac
    57961 atttggttat ccgaaagagg atcagcggtt atggcatgcg gtggccgaag ctacgggtct
    58021 gaaagctgaa agaacgctgt ttattgatga cagcgaagcg attctcgatg ctgccgcgca
    58081 atttggtatt cgttactgcc tcggcgtgac taatcctgat tccgggattg ccgagaaaca
    58141 gtatcaacgc catccgtcac tgaatgacta ccgccgcctg atcccctcgc taatgtgaag
    58201 gagacgccat gaaagaaaaa cctgctgttg aggttcgact ggataaatgg ctatgggctg
    58261 cccgttttta taaaacccgc gcgctggccc gtgaaatgat tgaaggcggt aaggtgcatt
    58321 acaacgggca gcgcagcaag ccgagcaaaa tcgtcgagct gaatgccacg ctcactctgc
    58381 gccagggaaa tgacgaacgc acggtgattg taaaggcgat tactgaacag cgtcgccccg
    58441 ccagcgaggc agccttgctg tatgaagaga ctgcggaaag tgtagagaaa cgcgaaaaaa
    58501 tggcgatggc acgtaaactt aatgccttaa ccatgccgca cccggaccga cgcccggaca
    58561 aaaaagagcg ccgcgacctg ttacgattta aacacggcga cagtgaataa ctgtcacctg
    58621 caagagagat gattatgccg caacatgacc aattacatcg ctatctgttt gaaaattttg
    58681 ccgttcgcgg cgaactggta accgtttcgg aaaccctgca acagatcctt gagaaccacg
    58741 attatccgca gcccgttaaa aacgtgctgg cagaactgct ggttgcgacc agcctgttaa
    58801 ccgctacgct gaagtttgat ggtgatatca ccgtacagct gcaaggcgac ggtccgatga
    58861 atctggcggt tattaacggt aacaataacc agcaaatgcg cggtgtggcg cgcgtgcagg
    58921 gagaaattcc agaaaatgcc gacctgaaaa cgctggtcgg caatggttac gtggtgatca
    58981 ccattacccc gagcgaaggc gaacgctatc agggcgtggt tggtctggaa ggtgataccc
    59041 tggcggcctg cctggaagat tactttatgc gttctgaaca gctgccgacg cgcctgttta
    59101 ttcgcaccgg cgacgtagac ggcaaaccgg ctgcaggcgg tatgttgttg caggtaatgc
    59161 ctgcgcaaaa tgcccagcag gacgactttg accacctggc gacgctaacc gaaaccatca
    59221 aaaccgaaga actgctgacc ttaccggcaa acgaagtgtt gtggcgtttg tatcacgaag
    59281 aagaggtgac ggtttacgat ccgcaggatg tggagttcaa atgcacctgc tcgcgtgaac
    59341 gttgcgccga tgcgctgaaa acgctgcctg atgaagaagt tgatagcatc ctggcggaag
    59401 atggcgaaat tgacatgcat tgtgattact gcggtaacca ctatctgttc aatgcgatgg
    59461 atattgctga aatccgcaac aacgcgtctc cggcagatcc gcaagttcat taatgagtat
    59521 gtccggcaga gagggctctg ccggatttta cgacgtaatg gcctgatgtg ctgcgcttac
    59581 caggcctgtg aaataaatcg caaattattg ttggtcagat acagcgtcgt atccgttaaa
    59641 agagttgtca cttacttaag tttatttcca gcgtttcacc atctaccgtc agatcgcgcc
    59701 acgctttcgg cctcgtcccc agttttccat ccttcgcagc tgtgagtaat ttttctttcg
    59761 ttatttcgtt cggaagtaaa ctcatatcaa gcgcgtccca ttcttttctg tctgagtcaa
    59821 agccatagac aatgactctg tcatcattaa aagcaaacag gatccgctcc gcctgaccat
    59881 cactattcag atcttgctcg accaggacgc aggcgtcttt ttcaatacag gtcatcacgt
    59941 tatagcgatc ctggattaag gccgaccaga atgtcgcatc cggtttaaca gaaccagggg
    60001 caattaacac attttcggct aatacttttt ccgatacctg ttcctgaaga tgttgctctc
    60061 catctaacgc catcagcaga tcccgcgcgc gtttcgggtc tttcataaac ccggcatcgc
    60121 ttttcagcga ctcaagcgca gcacgtccat agcgaccgct ctgctcgagc atgtagatag
    60181 ttacctggtc tgacgtgttt ttgccgctct gataacgcgc catatggctg ttcacgctaa
    60241 tgcgcatact gtccagcacc ggcgaattaa gaagcaccag tatcaccaac accaataacg
    60301 aaaccgcaag gttcactttg ccctgaagaa caacgggatt ttgcccttta cgccagacga
    60361 tgctgacaaa ataccccagc gaccacacca gtaacaccag caccgccagc acgccctgca
    60421 agcggtcgac agtccagccg tactgagcga cccgcagcca taacgcccag gcggcgacga
    60481 acacatacag cggagcaacc agcaaagcgg ttttaatcag gcaacgcaac ggccctgtcc
    60541 agggaagtga cgctttttgc ggatcgcgga caatagccat taagatcaat tgcaaaaagg
    60601 ccagcgttaa cagcaacccg gcggcggaga tgtggcgaga aatcgcgctc aggcccgtaa
    60661 acggcagagt gatgataaac atcagggtta gcaatgatac taacggcagc aaccccgtgg
    60721 cgattaacgt gaacaacttt tgaatagagt cgattaaacg tgactgtgtt cgcgcgagga
    60781 tcaccgccag cgcggtaacc agacctaacg tgagataaat aaaccagtcg gttgcaaaaa
    60841 agagcgtatt gaaaaacgtg atgccaacaa gtttaaacaa ctcactccac agtaaaagca
    60901 ccagccacgt caagccattg gcgagaaaaa tcaccagtaa tataagtacg ttatgccata
    60961 ctgactggta aaaatagcga tagcggctac tgtcattgcg gatgcgaagg ctttgttgta
    61021 tccacggtag caacaacatt gccatcagca gcagatagca accaaaatcc caaagcgcct
    61081 tttcagctct ccaggggttc atgccatcag tttgccactt cagccagccg ctcatcccca
    61141 gcgtggcaat aaacaccagc gccagccatc cccaaaggcg cttctgttta aaagagatca
    61201 cggagaagag taaaacggat gaaaacgcaa cggtcgcagg cacgccataa acaatccaac
    61261 tgtgattttt tccggcaagc caggcgatca acaggtagca aaccagcccc tgaagtaatc
    61321 cggtagcaat catcccccag cgtgttgccg gtgaaagttc aacgttatcc atgttggtta
    61381 tccagaatca aaaggtgggt taattatcgc atccgggcag tagtattttg cttttttcag
    61441 aaaataatca aaaaaagtta gcgtggtgaa tcgatacttt accgattgaa tttgcatcaa
    61501 tttcattaag gaatgcgatt ccactcacaa tattcccgcc atacaaacca agatttaacc
    61561 ttttgagaac attttccaca cctaaaatgc tatttctgcg ataatagcaa ccgtttcgtg
    61621 acaggaatca cggagttttt tgtcaaatat gaatttctcc agatacgtaa atctatgagc
    61681 cttgtcgcgg ttaacacccc caaaaagact ttactattca ggcaatacat attggctaag
    61741 gagcagtgaa atgcgcgtta acaatggttt gaccccgcaa gaactcgagg cttatggtat
    61801 cagtgacgta catgatatcg tttacaaccc aagctacgac ctgctgtatc aggaagagct
    61861 cgatccgagc ctgacaggtt atgagcgcgg ggtgttaact aatctgggtg ccgttgccgt
    61921 cgataccggg atcttcaccg gtcgttcacc aaaagataag tatatcgtcc gtgacgatac
    61981 cactcgcgat actttctggt gggcagacaa aggcaaaggt aagaacgaca acaaacctct
    62041 ctctccggaa acctggcaac atctgaaagg cctggtgacc aaacagcttt ccggcaaacg
    62101 tctgttcgtt gtcgacgcct tctgtggtgc gaacccggat actcgtcttt ccgtccgttt
    62161 catcaccgaa gtggcctggc aggcgcattt tgtcaaaaac atgtttattc gcccgagcga
    62221 tgaagagctg gcaggtttca aaccagactt tatcgttatg aacggcgcga agtgcactaa
    62281 cccgcagtgg aaagaacagg gtctgaattc cgaaaacttc gtggcgttta acctgaccga
    62341 gcgcatgcag ctgattggcg gcacctggta cggcggcgaa atgaagaaag ggatgttctc
    62401 gatgatgaac tacctgctgc cgctgaaagg tatcgcttct atgcactgct ccgccaacgt
    62461 tggcgagaaa ggcgatgttg cggtgttctt cggcctttcc ggcaccggta aaaccaccct
    62521 ttccaccgac ccgaaacgtc gcctgattgg cgatgacgaa cacggctggg acgatgacgg
    62581 cgtgtttaac ttcgaaggcg gctgctacgc aaaaactatc aagctgtcga aagaagcgga
    62641 acctgaaatc tacaacgcta tccgtcgtga tgcgttgctg gaaaacgtca ccgtgcgtga
    62701 agatggcact atcgactttg atgatggttc aaaaaccgag aacacccgcg tttcttatcc
    62761 gatctatcac atcgataaca ttgttaagcc ggtttccaaa gcgggccacg cgactaaggt
    62821 tatcttcctg actgctgatg ctttcggcgt gttgccgccg gtttctcgcc tgactgccga
    62881 tcaaacccag tatcacttcc tctctggctt caccgccaaa ctggccggta ctgagcgtgg
    62941 catcaccgaa ccgacgccaa ccttctccgc ttgcttcggc gcggcgttcc tgtcgctgca
    63001 cccaattcag tacgcagaag tgctggtaaa acgtatgcaa gcggcaggtg cgcaggcgta
    63061 tctggttaac accggctgga acggcactgg aaaacgtatc tcgattaaag atacccgcgc
    63121 tattatcgac gccatcctca acggttcgct ggataacgca gaaactttca ctctgccgat
    63181 gtttaacctg gcgatcccaa ccgaactgcc gggcgtagac acgaagattc tcgatccgcg
    63241 taacacctac gcttccccgg aacagtggca ggaaaaagcc gaaaccctgg cgaaactgtt
    63301 tatcgacaac ttcgataaat acaccgacac ccctgcgggt gccgcgctgg tagcggctgg
    63361 tccgaagctg taatcagctg atatctcatt gaccccttat ccaaacggat aaggggtttt
    63421 tttatatcta attagctacc aatatgtaca cggtacactt ataattatca atagaccgca
    63481 ggatgcatta tgttcacctt tattgaacta caaggattta gcaaacgccg ccagttattg
    63541 ctacctgatg atgagttccg gatttttcag gaactactca ttcaagatcc cactgccggt
    63601 gacattatcg caggcacagg cagttttaga aaaattcgct ggaaccgttc tggaatgggg
    63661 aaacggggtg gcattcggat aatttattac aacgtaacac gcaatggccg aatttatctg
    63721 gcgttgattt atcctaaaaa tgaacaggat gatttaacag aagaacaaag aaaggcattg
    63781 aaactattgt ccgagaagct gctttaatgt tgaagctcca caaattccac catggtcgaa
    63841 atgccaacgg agacattgaa aacagatgaa agatgaatta tttgctgatc ttcttgccag
    63901 cgcagaagaa atggtacgca tcgaaaaggg tgaagaaacg cctgaaccag agcacgtcca
    63961 tacttttagc gagatcgatg taaaagcgat ccgtgaagca acgggtttac gtcagcaaga
    64021 ttttgctgtc gccgttgggg taagttatga cctggtcaaa agctgggaaa ccaaacgccg
    64081 tcaacctacc ggagcacctc gaaaattgct cctgcttttg caagcgaatc ctttcattat
    64141 caatcaattg aaaacaatct aaaaacggga ggcaccttcg cctcccgttt atttaccctt
    64201 cttttgtcgt gccctgcgcc cgcgttaccg gcactggcag ccaggcgcga atggaaagcc
    64261 cgccccgctc gctggtgcca agctccagca tcccgttatg gttatccacg atacgctgca
    64321 caattgccag ccctaatccc gtgccgctaa tggtgcgcgc actgtcgccg cggacaaacg
    64381 gctggaacag gtgcttacgt tgttccggcg caattcccgg accgtcatct tccacctgga
    64441 accaggcgcg attcggctcc gtcccgctgc tgactttgat ccagccattg ccgtaacggg
    64501 cggcgttgac caccatattc gccaccgcgc gtttgatcga cagcgggtgc attttcactt
    64561 caatgctgcc ggggtaaagc gcggtttcaa tttcccgctc atagccactt tcggcagcaa
    64621 taacctcacc gagtactgcg ttgagatccg ccatttccat cggcatctcc tgtccggtgc
    64681 gcaggtagtc gataaactgc tcaatgatgg cattgcactc ctcgatatct ttattgatcg
    64741 attctgccag atagccatcc tgctcgctca tcatctcggt cgccaggcga atacgcgtca
    64801 gcggtgtacg caagtcgtga cttacccccg ccatcagcag cgtgcggtca tccgccagtt
    64861 gcttaacacc agccgccata tggttaaagg cgcgggtaac ggaacgcacc tcggacgcgc
    64921 catactcacg cagcggcggc ggaataatcc ctttaccgac ctgcaaagca gcatgttcga
    64981 gatcgaccaa cggtcggttc tggatacgaa taaacagcca cgccccgcct atcgccaata
    65041 gcataatcgc cagcgtatag cggaacagcg gagagaaatc gccctgatga atttcggtca
    65101 gcggcacgcg tacccagata ttgggcgaca gccaggtttt cagccagacg acaggcgaac
    65161 ttttgttgac ctcaacgcgc acttccgtcg ggccgcccag ttgctgcgcc atctgatggc
    65221 ttaagaattc atagtgttgc gcccaacgca gacctgcctc ttcggcagcc tcgttggagt
    65281 agagagagat ccccagctca cggtagatct cccgacggaa agcgggaggc acaaccaact
    65341 gcgtgccgtc ctccagttgc agtttgtcgg tcatcaacat acgcacttcg tacgcgagga
    65401 ctttattaaa ctgctggagg ctcggcaaaa tcgcgaagtt cagcaccacc agataagtcg
    65461 tcaccaggct ggcgaacagc aaggtgacga tgagcaataa cgtacgggca aatgaacttc
    65521 gtggcgagaa gcgcaatcgc ctcatgcttt agagccgtcc ggtacaaaga cgtagcccag
    65581 accccagacg gtctgaatgt aacgcggatg cgctggatct tcttccacca tgcggcgcag
    65641 acgcgaaatc tgcacgtcga tggagcgttc cattgcggaa tattcacgac cacgggcaag
    65701 gttcatcagc ttatcgcggg agagcggctc acgcggatgg ctgaccagtg ccttcagtac
    65761 cgcaaactca ccgctggtga gcggcatcgg ctcgtcttcg cggaacattt cgcgcgtacc
    65821 gaggttaagt ttgaacttac cgaaagcaat taccgcctct tcctgtgacg gtgcgcctgg
    65881 tagctcgttc gcctgacgac gcagcaccgc acggatacgg gccagcagtt cacgcgggtt
    65941 aaacggtttt ggaatgtaat cgtcagcgcc aatctccagg ccaacgatac gatccacttc
    66001 ttcccctttc gccgtcacca taatgatcgg catcgggttg ctctgactac gaagacgtcg
    66061 gcaaatcgac aagccatctt caccaggtaa cattaaatcc agtaccataa gatggaaaga
    66121 ttcacgagtc agcaggcgat ccatctgttc tgcattagcg acgcttcgaa cctggaagcc
    66181 ttgttcggtg agataacgtt ccagcagcgc acgcaggcgc atgtcgtcat cgaccaccag
    66241 aatcttgtag ttctcttgca ttgtttgtac tcccaaaggt tcgcaacaat ttgtaagcgt
    66301 gtattcttaa aaaagctcac gttcgtcacc agctaaatct ggtatgaatt tcagcctaaa
    66361 ttgttacaaa gcatattaaa cagcagctta agtatacaat ttattcggcg aaacattatt
    66421 gattctgttg atatgatcac gttataccca atgtgcgcat tatcaaacag acaaagggaa
    66481 tcaacgagat gaaaacgccc ctggttaccc gggaagggta tgaaaaactc aaacaagagc
    66541 ttaattatct ctggcgtgaa gaacgcccgg aggtcacaaa aaaggtgacc tgggccgcaa
    66601 gtctgggcga ccgcagcgaa aatgctgact atcagtataa taaaaagcgt ctgcgtgaaa
    66661 tcgaccgtcg cgtgcgctat ctcactaaat gcctggaaaa tctcaaaatc gtcgattact
    66721 cccctcagca ggaaggcaaa gtcttttttg gcgcgtgggt ggagattgaa aacgacgatg
    66781 gcgtgactca ccgtttccgt attgtcggct acgatgaaat ttttggccgt aaagattaca
    66841 tctctatcga ttccccgatg gcccgcgcat tgctgaaaaa agaagtcggc gatctggcgg
    66901 tggtgaatac ccctgccggg gaagcgagct ggtatgttaa tgctatcgag tacgtgaaac
    66961 cgtaaggaag agtcttaacc tcctgccgat ggctggcatt tttgccagcc agtccgtata
    67021 actatcccct gatttttgat ccgaaaagat gaactcaaac catgatgaat gattcgttct
    67081 gccgcattat tgcgggtgaa attcaggcgc gcccggaaca ggttgacgct gccgttcgcc
    67141 tgcttgacga agggaatacc gtgccgttta tcgcacgtta tcgtaaggaa atcaccggcg
    67201 gtctggatga cacgcagctg cgtaatctgg aaacgcgtct gagctatctg cgcgagctgg
    67261 aagagagacg tcaggcgatc ctcaaatcca tttccgagca aggcaaactc accgatgatc
    67321 tggcgaaggc catcaacgcc accctaagca aaaccgaact cgaagacctc tacctgccct
    67381 acaaacctaa acgccgcacc cgcgggcaaa tcgccattga agcagggctt gagccgttgg
    67441 ctgacctgct gtggagcgat ccgtcacaca cgccagaagt cgccgctgca caatatgttg
    67501 atgccgataa aggcgtggca gataccaaag ccgcgctgga tggcgcgcgc tatatcctga
    67561 tggaacggtt tgccgaagat gccgcgctgc tggcgaaagt gcgtgattat ctgtggaaga
    67621 acgcgcattt ggtttctacg gtggtgagcg gtaaagaaga ggaaggggcg aaattccgcg
    67681 actatttcga tcatcacgaa ccgttgtcca cggtgccttc tcaccgcgcg ctggcgatgt
    67741 tccgtgggcg taacgaaggc gtactccagc tttcgctgaa tgccgatccg cagttcgaag
    67801 agccgccaaa agagagctat tgcgagcaaa tcatcatgga tcatctcggc ctgcgcctga
    67861 acaatgctcc ggcggatagc tggcgcaaag gcgtggtgag ctggacctgg cgcatcaagg
    67921 tgctgatgca tctggaaacc gaactgatgg gcaccgtgcg cgaacgcgcg gaagatgaag
    67981 caatcaacgt ctttgcccgt aacctgcacg atctgctgat ggcggcccct gccggactgc
    68041 gtgccacgat gggcctcgat ccgggtctgc gtaccggggt aaaagtagcg gtggtcgatg
    68101 ccactggcaa actggtggcg accgatacca tttacccgca caccggacaa acagcaaaag
    68161 cagcgatgac cgttgctgcc ttgtgtgaaa agcataacgt tgaactggtg gcgatcggca
    68221 acggtacagc ttcccgcgaa accgaacgtt tctatctcga cgtgcagaag cagttcccga
    68281 aagtgaccgc gcagaaagtg atcgtcagcg aagctggcgc gtcggtttac tcggcttccg
    68341 agctggcagc gcaggagttc ccggatctcg acgtttcgct gcgtggtgcg gtatctatcg
    68401 cccgccgttt gcaggatccg ctggcggagc tggtgaaaat cgatccgaaa tctatcggcg
    68461 taggtcagta tcagcatgac gtcagccaga cgcaactggc ccgcaaactg gatgcagtag
    68521 tagaagactg cgtaaacgcc gttggcgtcg atctcaacac cgcttctgtt ccgctattaa
    68581 cccgcgtggc gggcctgacg cgcatgatgg cgcaaaacat cgtggcctgg cgcgatgaga
    68641 acggccagtt caagaaccgt cagcaactgc tgaaagtcag ccgtctgggg ccgaaagcct
    68701 tcgagcagtg cgcgggcttc ctgcgcatta accacggtga taacccgctg gacgcctcta
    68761 ccgttcaccc ggaagcctat ccggtggtgg aacgcattct ggcagcaaca cagcaggcac
    68821 tgaaagatct gatgggtaac agcagcgaac tgcgtaacct gaaagcgtct gactttactg
    68881 atgaaaaatt cggtgtgccg acagtaactg acatcatcaa agagctggaa aaaccgggtc
    68941 gcgatccgcg tccggaattt aaaaccgctc agtttgccga tggcgtcgag acaatgaacg
    69001 acctgcaacc gggtatgatc ctcgaaggcg ctgtcaccaa cgtcaccaac tttggcgcgt
    69061 ttgtcgatat tggcgtgcat caggacggcc tggttcacat ctcttcattg tcgaacaagt
    69121 ttgtggaaga tccgcatacc gtggtgaaag cgggcgacat tgtgaaggtg aaagtgctgg
    69181 aagtggatct tcagcgtaaa cgtatcgccc tgaccatgcg tctggacgag cagcctggcg
    69241 aaaccaacgc ccgtcgcggc ggcggtaatg aacgcccgca aaacaaccgc ccggcagcca
    69301 aaccacgcgg tcgtgaagcg cagcctgccg gtaatagcgc gatgatggat gcgctggcgg
    69361 cggcaatgag caaaaaacgt taaacgccct gattgacccc taaacctgcg cgataaggtg
    69421 tccacgccgc atctggcaac gaacgccttg tctgacataa aagtgccgga gaatatctcc
    69481 ggcattttta ttccacagcc aaactcacaa tatattccgg caacatttat cacttcatta
    69541 acaactgaaa ccttaattaa acattagcca gtcctggtaa ttcgctattc gaattatatt
    69601 ttcgctgcga tataaccttg agccacatca acattgagtc agattattat tcaaaccaac
    69661 attcgcacac attttaagta ttgctgatag aaaccattct cattatcatt gtgctgttga
    69721 ttatttaatc tctccttcgt tggcaaatca tctggtctca tgtcgctgtc aaacgcccca
    69781 tgaggtagtt atccagttaa tgagaaacaa gtaggcacct atgcaataca ctccagatac
    69841 tgcgtggaaa atcactggct tttcccgtga aatcagcccg gcatatcgcc aaaaactgct
    69901 ttctcttggc atgttacctg gctcctcttt taatgtggtg cgcgtcgctc cactcggcga
    69961 ccccattcat atcgaaaccc gtcgtgtgag cctggtatta cgcaaaaaag atctggcctt
    70021 attagaagtg gaagcggttt cctgttaata cggtgataac aacaatgaaa aaattaacca
    70081 taggcttaat tggtaatcca aattctggca agacaacctt atttaaccag ctcactggcg
    70141 cacgtcagcg tgtaggtaac tgggctggcg ttaccgtcga acgtaaagaa gggcaattct
    70201 ccaccaccga tcatcaggtc acgctggtgg acctgcccgg cacctattct ctgaccacca
    70261 tctcatcgca gacctcgctc gatgagcaaa tcgcctgtca ctacattttg agtggcgacg
    70321 ccgacctgct gattaacgtg gtggatgcgt ctaaccttga gcgtaacctg tacctgacgc
    70381 tacaactgct ggaactcggc attccctgca tcgtggcact gaacatgctc gacattgccg
    70441 agaagcaaaa tattcgtatt gaaattgatg ctctgtcggc gcgtcttggc tgcccagtga
    70501 tcccgctggt ttcaacccgt ggtcgcggta ttgaagcgct caagctggcg attgatcgct
    70561 ataaagctaa cgagaatgtg gaactggtgc attacgcaca gccgctactc aacgaagcag
    70621 attcactggc aaaagtgatg ccttccgaca tcccgctgaa acaacgtcgc tggctgggcc
    70681 tgcaaatgct ggaaggcgat atctacagcc gcgcctacgc cggtgaagcg tcgcagcatc
    70741 tggatgccgc cctcgaccgt ctgcgtaatg agatggacga tccggcgctg cacattgccg
    70801 atgcgcgtta ccagtgcatt gctgccatct gtgatgtggt aagcaacacc ctgacggcag
    70861 aacccagccg tttcaccact gcggtagata aaatcgtgct caaccgtttc ctcggtctgc
    70921 cgattttcct ctttgtgatg tacctgatgt tcctgctggc tatcaacatc ggcggggcgt
    70981 tacagccgct gtttgacgtc ggctccgtgg cgctatttgt gcatggtatt caatggattg
    71041 gctacacgct ccacttcccg gactggctga ctatcttcct cgcccagggc ctgggtggcg
    71101 gcattaacac cgtgctgcca ctggtgccgc agattggcat gatgtacctg ttcctctcct
    71161 tccttgagga ctccgggtat atggcgcgtg cggcgtttgt gatggaccgt ctgatgcagg
    71221 cgctgggctt gccggggaaa tcctttgtgc cgttgatcgt cggtttcggt tgtaacgtac
    71281 cgtcggtaat gggtgcacgt acgcttgatg caccgcgtga acgtctgatg accatcatga
    71341 tggcaccgtt tatgtcctgc ggcgcgcgtc tggctatctt cgcagtattc gcggctgcct
    71401 tcttcgggca gaacggtgcg ctggcggtct tctcgctgta tatgctgggt attgtgatgg
    71461 cggtgctgac tggcctgatg ctcaagtaca ccatcatgcg cggtgaagcg acgccgtttg
    71521 tcatggagtt gccggtctat catgtaccac acgttaaaag cctgattatc cagacctggc
    71581 agcgtctgaa aggcttcgtt ctgcgtgctg gtaaagtgat catcatcgtc agcattttcc
    71641 tgagcgcttt caacagcttc tcgctgagcg ggaaaatcgt cgataacatc aacgactcgg
    71701 cgctggcttc cgtcagccgg gtgatcaccc cggtcttcaa gccgattggc gtacatgaag
    71761 ataactggca ggcaacggtt ggcctgttta tgggcgcaat ggcgaaagaa gtggtggtgg
    71821 gtacgctcaa caccctctac accgcagaaa atattcagga cgaagagttc aatccggcgg
    71881 aattcaacct cggtgaagag ctgttcagtg cggtagatga aacctggcag agcctgaaag
    71941 acaccttcag ccttagcgta ctgatgaacc ccattgaagc tagcaaaggc gacggcgaaa
    72001 tgggtaccgg ggcgatgggc gtgatggatc agaaattcgg tagcgcagct gccgcataca
    72061 gctacctgat tttcgtcctg ctgtatgtac cgtgtatctc ggtgatgggg gctatcgccc
    72121 gtgaatcaag ccgtggctgg atgggcttct ccatcctgtg ggggctgaat atcgcttact
    72181 cactggcaac attgttctat caggtcgcca gctacagtca gcatccaact tacagcctgg
    72241 tgtgcattct ggcggttatc ctgtttaaca tcgtggttat cggtctgctg cgccgcgcgc
    72301 gtagccgggt ggatatcgaa ctgctggcaa cccgcaagtc ggtaagcagt tgctgcgcag
    72361 ccagcaccac cggtgattgc cattaatggc ttcacttatt caggtgcgcg atttgctggc
    72421 gttacggggc cgtatggaag cggcccagat aagccagaca ttgaacactc cacagccaat
    72481 gattaacgcc atgctgcaac aactggaaag tatgggcaaa gccgtgcgga ttcaggaaga
    72541 acctgacggc tgcctctctg gcagttgtaa aagctgcccg gaaggaaaag cctgtctgcg
    72601 cgagtggtgg gcgctgcgtt aaccttactc catcgcctgt tttggaaagc ccggtatgcg
    72661 tctgcatccg ggcttttttg cgtgcggctt tccataaaaa tgcaactctt gcagcacggc
    72721 gtaagttcct ttgaaagcat ctcgcaggga tgaaaactcg ctaatacaca ggagtgaagt
    72781 ggcgcgtaga gtcgcggcat tcaaacaaca ggtgaaggaa cgccatgagc aaaaagcaga
    72841 gttccacccc acacgatgcg ctgttcaaac tctttttacg ccaaccggac acggctcgtg
    72901 attttcttgc gtttcattta ccggcaccca ttcacgcgct ttgtgatatg aaaaccctca
    72961 agctggagtc gagcagcttt attgatgacg atctgcgtga aagctattcc gatgtgctgt
    73021 ggtcggtgaa aacggaacaa ggaccaggat acatctattg tctgattgaa catcaaagca
    73081 cctcaaacaa actgatcgca tttcgcatga tgcgttacgc tattgccgca atgcaaaatc
    73141 accttgatgc tggatacaaa acgttgccga tggtggtgct attgttgttt taccacggta
    73201 ttgaaagccc ctatccctat tcgctgtgtt ggctggattg tttcgccgat cccaaactgg
    73261 caaggcagct ttatgcctcc gcatttccgc tgattgatgt caccgtcatg cctgatgatg
    73321 aaatcatgca gcaccgacgt atggcgctgc tggagttaat tcaaaaacat attcgtcaac
    73381 gcgacctgat ggggctggta gagcaaatgg cctgcttatt aagtagtgga tacgctaatg
    73441 acagacaaat caaagggctg tttaattaca tactgcaaac cggcgacgcg gtacgtttta
    73501 acgattttat cgacggcgtt gccgaacgtt caccgaaaca caaggagagt ttaatgacaa
    73561 ttgcggaaag attgcggcag gagggggaac aatccaaagc cctgcatata gccaaaataa
    73621 tgcttgaatc cggagtccct cttacagaca tcatgcgctt taccggggtg tcagaagaag
    73681 agttggctgc ggcgagccag taaatttctg tctcgccatt tcaaaagttg cctacaccct
    73741 ctgcttcaac gccaccagca ggtgacaaaa ctcgaccgga tgcgaaataa acggcgcatg
    73801 ggccgctttg gcgaagatat atgattcgct gtgaggccaa agtttatcca gcatcggcac
    73861 cactttgcgc ggcaccagac cgtcgagata gccatacaat cgcaaaaacg gcatggacac
    73921 gttttgcagc ggctgacgga gatcgaccgt tttcaggatt tccagcccgc cattaagcac
    73981 gtcaacctcc ggcatcggta acgccagaac ggttttcttc aacgcccgcg catcctggcg
    74041 cgccgtttca gtccccatgg tttgtaacgc caggaaccgc tccactgtac gctgaaaatc
    74101 atcactgagt tgctgctgaa atcccgccag cacgtccggt tttatccccg gccactcgtc
    74161 acgagcacta aaacaaggtg acgacgccac ggtgaccagc gcctgaacac gctcgggatg
    74221 ggttaaaaca atttggcttg ccaccagccc gcccagactc cagcctaacc aaatggcttt
    74281 atcaggtgcc tgttgcagca cggcttcggc catatcagca agtgacagcg caccaaatcc
    74341 ccggctacgc ccgaagccgg gcaggtcaac aaggtgcagc gtaaaatgcg agctaagttc
    74401 ctcgtcaatg caacgccaca cttcggcatt cagtccccat ccgtgcagca gcacaagatg
    74461 aacattcccc tgacctttgg tctgccacca gatgttattc atccgctatt gttctctttt
    74521 gacttacaag gatgaacata tgctaacagt accgggatta tgctggctat gccgaatgcc
    74581 actggcgtta ggtcattggg ggatttgttc ggtctgttca cgcgccaccc gcacagataa
    74641 aacgttatgc ccacaatgtg gattacccgc cacacactcc catcttccct gcggtcgctg
    74701 cctgcaaaaa ccgccgccct ggcaaagact ggtcacggtt gccgactatg cgccgccgtt
    74761 aagtccgctt atccaccagc ttaaattttc ccggcgcagt gaaatcgcca gcgccctgtc
    74821 acgtctgtta ctattggaag tcttacacgc tcgtcgcacc accgggttgc aattgccgga
    74881 tcgcatcatc agcgttccgt tatggcagcg gcgtcactgg cgtcggggat ttaatcagag
    74941 cgatttgctg tgtcagccgt tatcacgctg gttgcactgc caatgggata gcgaagccgt
    75001 cacacgtaca cgggccactg cgacccagca ttttctcagt gcccggctgc gcaagcgcaa
    75061 cctgaaaaat gcctttcgtc ttgaattgcc cgtgcaaggt cgccatatgg tgattgtgga
    75121 tgatgtcgtt accaccggaa gtaccgtcgc agagattgcg cagttgcttt tacgcaatgg
    75181 tgcggcgact gtccaggtct ggtgcctttg tcgaaccttg tagagcctcg atgatgggcg
    75241 tattataacc aactaaaata gtcaactatt aggccattac tatgatccgt atttccgatg
    75301 ctgcacaagc gcactttgcc aaactgctgg caaatcagga agaagggaca caaatccgcg
    75361 tatttgtgat taaccctggc acgcctaacg ctgaatgtgg tgtttcttat tgtccgccgg
    75421 atgccgtgga agccaccgac actgccctga aatttgacct gctgaccgcg tatgttgatg
    75481 agttaagcgc tccttacctg gaagatgcag agatcgattt tgttaccgac cagttgggtt
    75541 ctcagctaac actgaaagcg cctaacgcca aaatgcgtaa agtggcagac gatgcaccac
    75601 tgatggagcg cgtggagtat atgctgcagt cgcagatcaa cccacagctt gctggtcacg
    75661 gtggccgcgt ttcactgatg gaaatcaccg aagacggtta cgccattctg caatttggcg
    75721 gcggctgtaa cggttgttcc atggtcgatg tgacgctgaa agaagggatc gagaagcagc
    75781 tgctaaacga attcccggag ctgaaaggtg tgcgcgatct caccgaacac cagcgcggcg
    75841 aacactccta ctactaagtt atcccctctt ttgtagattg cccgatgcga cgctaaagcg
    75901 tcatttcggg cctacgttga acatgtgcca gacgttggca cagcattgct ttggcaaatc
    75961 cctcccttcg tttccccgcg ataatatgac caacctctca taatttaaat ttaccccgct
    76021 ctggtgattc tcaaacgcca gatgttaccc gtatcattca catgggtacc aaacatactc
    76081 ctgacatctg actacaataa ttagttttag tgggtatcag tcgtggtgcc gcaatatctc
    76141 tgttcccgat tgggataatt agagtttgtc gtcagaaaat tgacgttacc cataacaaat
    76201 gaaaggccag gtaaatcatg ccattagtca ttgttgctat cggtgtaatc ttgttgttgc
    76261 tcctgatgat ccgcttcaaa atgaacggct tcatcgctct cgtcctcgtg gcgcttgctg
    76321 ttggattaat gcaaggaatg ccgctggata aagttattgg ctccatcaaa gccggtgtcg
    76381 gcgggacgct cggtagcctt gccctgatca tgggttttgg cgcaatgctg ggcaaaatgc
    76441 tggcagactg cggtggcgca caacgtatcg ccaccacgct gattgccaaa tttggtaaaa
    76501 aacacatcca gtgggcggtg gtactgaccg gttttaccgt tggttttgcc ctgttctatg
    76561 aagtgggctt tgtgctgatg ctgccgctgg tgtttaccat cgcggcttct gcgaatattc
    76621 cactgttgta tgttggtgta ccaatggcag ctgcactgtc tgtgacccac ggcttcctgc
    76681 caccgcatcc gggtccgact gcgattgcca ccattttcaa tgccgatatg ggtaaaaccc
    76741 tgctgtacgg tactattctg gcaatcccga ccgtgattct cgccggtccg gtttacgctc
    76801 gcgtgttgaa aggtatcgat aagccaattc cggaaggtct ctacagcgcg aaaaccttca
    76861 gcgaagaaga gatgccgagc tttggcgtca gcgtctggac ctctctggtg ccggtagtac
    76921 tgatggcgat gcgtgcgatt gccgaaatga tcctgccgaa aggtcacgct ttcctgccgg
    76981 tagcggagtt cctcggtgac ccggtaatgg ctacgctgat tgccgtgctg attgccatgt
    77041 tcacctttgg tctgaaccgt ggtcgttcaa tggatcagat taacgacacg ctggtttctt
    77101 ccatcaaaat cattgcgatg atgctgttga tcatcggtgg tggcggtgcg ttcaagcagg
    77161 tgctggtaga cagcggcgtg gacaaataca ttgcttccat gatgcacgaa accaacattt
    77221 ctccgctgct gatggcctgg tcgattgctg ccgtactgcg tatcgcgctg ggttccgcaa
    77281 ccgttgcggc aatcactgcg ggtggtatcg cggcaccgct gattgcaacg acgggtgtta
    77341 gcccggagct gatggttatt gcggtgggtt ccggtagtgt gattttctct catgtgaacg
    77401 atccgggctt ctggctgttc aaagagtact ttaacctgac tatcggcgag accatcaaat
    77461 cctggtcgat gctggaaacc attatctcgg tgtgcggtct ggtaggctgt ctgctgctga
    77521 atatggtgat ttgataacac tgactgccgg atgcatcgca tccggcatca accgcactct
    77581 acttcttctt cgctgcagct ctgcgccgtc tgtccaaatc cttcagcaac ttgttcacgc
    77641 catcatcggc aaacatcgac tcaagcgttg cggaaagctt gcgtcgccag tttttatact
    77701 ggtaactggt gccaggaata ttcaccggtt cggccatatc cagccagtct tccggctgta
    77761 gtcctaacag agcactgtta ctgtcggcaa tgtagcgctg caaaccacgg ttcagcgtcg
    77821 gcgtcatcga catcaacgat gccttatgcc cggcacgttt tggcagacaa ccatatttat
    77881 gcagtgcatc cagcagccct tgcttcgcca gttcgcgatc ctgatacaga ccgcgcagta
    77941 ccacttcatc cggatacagc cccagggttt tgcccaacgt tagatccccg ctctcccagt
    78001 aaccgcgcag cgtaggcagg tcatgtgtcg ccgcaaccgc catcgactgc tccggatacg
    78061 ctttcggtgc acggaacgtc ttctcgtggt cgttttcgaa atagagcact ttgtaggagt
    78121 acacaccgct gctgcgcagc ttaccgacaa tctctaccgg tacggtaccg agatcttcac
    78181 caatcaccat acagcgatga cgtttacttt cgagtgccag aatcgagagc agatcatcca
    78241 ccggatagtg aacatacgcg ccctgatctg ccgtctcgcc atacggtatc caccacaaac
    78301 gcagcatcga catcacatgg tcaattcgta atgcgccgca gttttgcata ttggcacgca
    78361 acagctcgat aaacggttca taggcacgcg cggtgatgat atgcgggtcc attggcggta
    78421 atccccagtt ctgccccaac gggccgagga tatccggcgg cgcgccaacc gacgctttca
    78481 ggcaatatag ttcacgatca caccaggttt ccgccccacc ttccgctacg ccaaccgcca
    78541 gatcacgata caagccaatc ggcatttcat agccctggct tatctcccag caggcggcaa
    78601 actggctgta agccagccac tgcaaccaga gataaaaatc gacgtcatca cgatgttctt
    78661 cgcagaactg acgcacttct ggtgaatcca cgttctgata catctctggc catgcaggcc
    78721 agccccagcg catttcgtcc tctttcactt gctgggcatg tagcgcatca aaggctgcct
    78781 gccagaacag gctgtcgccc tgctctgcaa caaactggcg aaacgcggtc atctgctcat
    78841 catcacgttg cgcgaaacct ttccacgcca ttcgtaatgc tgtcattttt agggcggtaa
    78901 ccgtggagta atcgacccag tcggcatcgc gcgcctgttg cagcgtctgt tgcgtggtcg
    78961 gcaactgcca ccaggcctga gcctcttcgc taagatggaa atcttcaacg gcgttaacgt
    79021 cgatataaat cacattcagc caacggcgag aagacgggct gtatgggctg gcactctccg
    79081 gatttgccgg atagagcgca tgaatcgggt tcaggccgat gaacgacccg ccacgttttg
    79141 ccacatccac cagcatcgct ttgagatcgc caaaatcccc aataccccag tttttttccg
    79201 atcgcagcgt ataaagctga acgcaggcac cccacagctt ttgtttattc agcaacgcct
    79261 gcggttcgta acagcgtttc ggggcgacaa tcacccggca atgcgcgcgc tggtcgtcct
    79321 gggtgagtgt cagcgtgtga taaccttccg gcagcttcgt cggtagattg aacgctttgc
    79381 cccccgttac atggcctttg tactgtgttc cttcttcggt ggtcagcagc cagctatatt
    79441 cgccgctgcc ctccaccacc atcggcattt ttttgccgct ggtataaacc atgacattcg
    79501 ggactggcgt taccgccact ttcgtggcgg tacgttgatg catcgcgtca agcaaacgcc
    79561 gtttggtttc ggcgctaatc gactgcggtt taccgtgggc attgatgtaa ttggggctaa
    79621 tccccgccgc cagcgcggca ttatccagac gtttgctttc catcgagctt ccttagcgtt
    79681 ttgcctgcca gatacgagcc tgataatcgc gaatagagcg atccgagcta aacataccgc
    79741 agcgggcggt attgaggatc gccgcgcgag tccaggcctc ctggtcgcgg tacagcacat
    79801 ccacctgctt ttgtgcctca acataggctg cgaaatccgc catcaccaga tacggatcgc
    79861 cgccttgttt gccgatactg tgcagcatct ggtcgaaggc atgcttatcg ccgtcgctgt
    79921 atttaccgct ttccagctct ttcaatactg cgtccagcac cttatctttc ttccgccatt
    79981 tcaccgggtc gtagcctttg gccagaattg ccttcacttg ttccacggta tgaccaaaaa
    80041 taaagatatt ttcttcaccg actttctcgg cgatttcaac gttcgcacca tccagcgtac
    80101 cgacagtaag cgcaccattg agcgccagtt tcatattgcc ggtaccggaa gcttctttac
    80161 ctgcagtcga aatttgttcg gagatatccg ccgccgggat cagtttttcc gccgccgaaa
    80221 cgcaataatc cggcaggaac accaccttca acttatcgcc aaccagtgga tcgttgttga
    80281 tcacgtcagc cactttgttg atcgcaaaga taatattctt cgccaggtag tagcccggtg
    80341 ccgctttcgc gccgaagagg aagacgcgcg gtacgcgatc agcctgcggg ttttcacgaa
    80401 tttctttgta caacgccaga atatgcagca gattcaggtg ctggcgtttg tactcatgca
    80461 gacgtttgat ctgaatatcg aaaatcgcct gtggattgat ctcaatacca gtacgaactt
    80521 tcacaaactc cgccagacgg actttattcg cctgcttgat ctcgcgatat tgctgacgga
    80581 atttcgcatc atcagcgaat ttttccagat tgatcagctg atcgagatcg ttagcccact
    80641 ctttttgcag tgatttatcc aacagagccg ccagtgcagg gttgcactgt ttgatccagc
    80701 gacgtggggt aataccgttg gtgacgttat ggaatttgtt cggccatagc tggtgatatt
    80761 ccgggaacag atctttcacc accagatccg agtgcagcgc cgcaacaccg ttcaccgcga
    80821 aaccgccaac cacacacagg ttcgccatat gcacttgttt atcgtgcacc accgccagtt
    80881 tggcccacac tttttcatcg cccggccagg ttttctccac cagcgtttta aagcgagtat
    80941 taatttcgtt aataatctgc atatggcgcg gcagtaagcc tttcaccagt ttcacatccc
    81001 agcgttccag cgcttctggc atcagggtat ggttggtgta agcgaaagtt ttgctggtaa
    81061 tggcccaggc gtcatcccag ctcatctggt gctcatcgat cagcacgcgc agcagttctg
    81121 gaatcgcgat agttgggtgg gtatcgttca gctgaataac ttcgtaatcc gccagttcgt
    81181 gcagtttacg ccccgccaga tgatggcgac gcaaaatatc cgctaccgaa caggcacact
    81241 ggaagtactg ctgcatcaag cgcagctttt taccggcagt atggttgtcg tttggataga
    81301 gaactttggt cagtttttcc gcattgatgc cctgctgttc ggcacgcagg aaatcaccgt
    81361 cgttaaattt agtcagatca aacggatgcg cgtgcgtcgc ctgccacaga cgcagcggct
    81421 gcgccacgcc attacgatag ccgacaacgg ggagatccca cgcttgaccg gtaatggtaa
    81481 actccggctc ccagcgtccg tctttcgtca ctttaccgcc aatccctacc tgcacatcca
    81541 gtgcttcgtt gtggcggaac cacgggtagt tactgcgatg ccagtcatcc ggcgcttcaa
    81601 cctgtttgcc atcgacaaaa gactggcgga acaaaccata ttgatagttc agaccgtaac
    81661 ccgtcgcaga ctgaccgaca gttgccattg agtcgaggaa gcacgccgcc agacgtccca
    81721 gaccaccgtt acccagcgcc gggtcgatct cttcttccag caggtccgtc agattgatgt
    81781 cataagcctt caacgaatcc tgtacatcct gataccagcc gagattcaac aggttgttgc
    81841 ccgtcaggcg accaatcaaa aactccattg agatgtagtt aacatgtcgc tgattcgcca
    81901 ccggcttggc gaatggctga gcacgcagca tttcggccag tgcttcactc actgctagcc
    81961 accactggcg aggagtcatt tcagccgcag aatttaagcc ataacgctgc cactgacgtg
    82021 aaagcgcttc ctgaaattgc ttatcgttaa aaataggttg tgacatagga gttccacttt
    82081 tcttagattt tcaacacaac gttatcgcta gtttgccagg ctcgatgttg accttcctca
    82141 tcctgcgggg gattaggcag ggaggagttg cggggatgag caaggaaatg tgatctcaac
    82201 cacttaaagc tagtgcaaac cacaggatta gcagcaaatc aatgcaatac agcgcagaaa
    82261 atctgtatct aagtgcaaaa aatggccgtt gcgtattttc aaaaagcgga aggtaactct
    82321 ataaattaag taaaggagtg aaacagtttc ataagtaaaa tatccagtgt gctccatctc
    82381 attcttaata gatttattaa gatcatcttt ttagatggca ctttcatcag gaatgaagaa
    82441 gaaacccttg cttaaatgaa tctgatgaac ataagggaaa ccagtattca cgctggatca
    82501 gcgtcgtttt aggtgagttg ttaataaaga tttggaattg tgacacagtg caaattcaga
    82561 cacataaaaa aacgtcatcg cttgcattag aaaggtttct ggccgacctt ataaccatta
    82621 attacgaagc gcaaaaaaaa taatatttcc tcattttcca cagtgaagtg attaactatg
    82681 ctgattccgt caaaactaag tcgtccggtt cgactcgacc ataccgtggt tcgtgagcgc
    82741 ctgctggcta aactttccgg cgcgaacaac ttccggctgg cgctgatcac gagtcctgcg
    82801 ggctacggaa agaccaccct catttcccag tgggcggcag gcaaaaacga tatcggctgg
    82861 tactcgctgg atgaaggtga taaccagcaa gagcgtttcg ccagctatct cattgccgcc
    82921 gtgcagcagg caaccaacgg tcactgtgcg atatgtgaga cgatggcgca aaaacggcaa
    82981 tatgccagcc tgacgtcact cttcgcccag cttttcattg agctggcgga atggcatagc
    83041 ccactttatc tggtcatcga tgactatcat ctgatcacta atccagtgat ccacgagtca
    83101 atgcgcttct ttattcgcca tcaaccagaa aatctcaccc tggtggtgtt gtcacgcaac
    83161 cttccgcaac tgggcattgc caatctgcgt gttcgtgatc aactgctgga aattggcagt
    83221 cagcaactgg catttaccca tcaggaagcg aagcagtttt ttgattgccg tctgtcatcg
    83281 ccgattgaag ccgcagaaag cagtcggatt tgcgatgacg tttccggttg ggcgacggca
    83341 ctacagctaa tcgccctctc cgcccggcag aatacccact cagcccataa gtcggcacgc
    83401 cgcctggcgg gaatcaatgc cagccatctt tcggattatc tggtcgatga ggttttggat
    83461 aacgtcgatc tcgcaacgcg ccattttctg ttgaaaagcg ccattttgcg ctcaatgaac
    83521 gatgcactca tcacccgtgt gaccggcgaa gaaaacgggc aaatgcgcct cgaagagatt
    83581 gagcgtcagg ggctgttttt acagcggatg gatgataccg gcgagtggtt ctgctatcac
    83641 ccgctgtttg gcaacttcct gcgccagcgc tgccagtggg aactggcggc ggagctgccg
    83701 gaaatccacc gtgccgccgc agaaagctgg atggcccagg gatttcccag cgaagcgatt
    83761 catcatgcgc tggcggcagg cgatgcgctg atgctgcgcg atattctgct taatcacgcc
    83821 tggagtttgt tcaaccatag cgaactgtcg ctgctggaag agtcgcttaa ggccctgccg
    83881 tgggacagtt tgctggaaaa tccgcagtta gtgttattgc aggcgtggct aatgcaaagc
    83941 caacatcgct atggcgaagt taacaccctg ctagcccgtg ctgaacatga aatcaaggac
    84001 atcagagaag gcaccatgca cgcagaattt aacgctctgc gcgcccaggt ggcgattaac
    84061 gatggtaatc cggatgaagc ggaacggctg gcaaaactgg cactggaaga gctgccgccg
    84121 ggctggttct atagccgcat tgtggcaacc tcagtgctgg gtgaagtgct gcactgcaaa
    84181 ggcgaattga cccgctcact ggcgctaatg cagcaaaccg aacagatggc acgccagcac
    84241 gacgtctggc actacgcctt atggagttta atccagcaaa gtgaaattct gtttgcccaa
    84301 gggttcctgc aaaccgcgtg ggaaacacag gaaaaagcgt tccagctgat caacgagcag
    84361 catctggaac agctgccgat gcatgagttt ctggtgcgca tccgagcaca actgttatgg
    84421 gcctgggcac ggctggatga agccgaagcg tcggcgcgta gcgggattga agtcttgtcg
    84481 tcttatcagc cacagcaaca gcttcagtgc ctggcaatgt tgattcaatg ctcgctggcc
    84541 cgtggtgatt tagataacgc ccgtagccag ctgaaccgtc tggaaaacct gctggggaat
    84601 ggcaaatatc acagcgactg gatctctaac gccaacaaag tccgggtgat ttactggcaa
    84661 atgaccggcg ataaagccgc cgctgccaac tggttgcgtc atacggctaa accggagttt
    84721 gcgaacaacc acttcctgca aggtcaatgg cgcaacattg cccgcgcaca aatcttgctg
    84781 ggcgagtttg aacctgcgga aattgttctc gaagaactca atgaaaatgc ccgcagtctg
    84841 cggttgatga gcgatctcaa ccgtaacctg ttgctgctta atcaactgta ctggcaggcc
    84901 ggacgtaaaa gtgacgccca gcgcgtgttg ctggacgcat taaaactggc gaatcgcacc
    84961 ggatttatca gccattttgt catcgaaggc gaagcgatgg cgcaacaact gcgccagctg
    85021 attcaactca atacgctgcc ggaactggaa cagcatcgcg cacagcgtat tctgcgagaa
    85081 atcaatcaac atcatcggca taaattcgcc catttcgatg agaatttcgt tgaacgtctg
    85141 ctaaatcatc cggaagtgcc agaactgatc cgcaccagcc cgttgaccca acgtgaatgg
    85201 caggtcctgg ggctgattta ctccggttac agcaatgaac aaattgcggg cgaactggaa
    85261 gtggcggcaa ccaccatcaa gacgcatatc cgcaatctgt accaaaaact cggcgtcgcc
    85321 caccgtcagg ctgccgtgca gcacgcgcag aaactgctga agatgatggg ttatggggtg
    85381 tgagtttagc cggataatgc attggatccg gtttacatct ttacatcatt caatactcac
    85441 ccgcgttacg ccatctgttt ctatcaaacc aaaccgcacc ggcaagaaac gctccaccac
    85501 cgcgatattg gtcagcagat ggcatgaagg atgggcgacc ttaaattctc ccgcgcccgc
    85561 cagcgccatc ggcagtacca gttggtcggc gagatattcc cccaccgccg ctgggcttgc
    85621 caggtagcgt ttcacctctt tcactaactg cgcggctacc acctcggcac tgacgcgctt
    85681 ttcaccgacg acgaaaaagc gttcagtgat attttcactt tcgacttcca gcgagacggt
    85741 attacccggc ccctggtcgc gcggcaggtt atgaatattc tgttcatgca gggaaaaact
    85801 ccccgccagt gtagcgattt cacgctcagc aacatggcgc ggcacaccag ctaatagaac
    85861 ttctccacac atctgcacaa tgttcccgcg ctcgccaagt tgcaaggtgt taaacgatgc
    85921 cacgggcgag acttccgttg ccaccacacc gcctccggca ggataaaaac cgtgacgtaa
    85981 cagcgtggtt tgctgatgaa ttcctatttt cgccagcagc ggctccagca cccggcggat
    86041 aaaatccgca ggcggggccg acgggttatc ggtgcctccg ctcacttcaa cacgcgaagg
    86101 tccatcggca aaccacagcg cgggcagcac cgtttgcagc accagcgtac agcttccggc
    86161 actaccgata gcaaagcggt actcgccgcc gcgcacggtg ccaggccgga agaccagacg
    86221 ctgctacccc agctccgcgc cttccaccgt tgccctacaa atttccgcag ccgcttttac
    86281 cgcggtcaga tgctggcgca acagccccgg tttcgctcgc ccggcacgaa tgctggtgat
    86341 ggtaaatggc tggccggtta tcatcgacag gctcagcgcc gagcgcagga tctgcccgcc
    86401 accttcgccc tgtgcgccat ccagcgcaat catccttttc atcatttatc cttttacgca
    86461 caccacctga cgcagggtat agataacttc caccagatcg ctttgtgccg ccatcaccgc
    86521 atcaatatct ttatacgcca tcgggatttc gtcgatcact tcggcatctt tacggcattc
    86581 cacatgcgcg gtggcacgaa tttgatcttc cacgctgaac agttttttcg ctttagtacg
    86641 gctcattacc cgcccggcac cgtggctgca cgaacagaac gactcttcat ttcccagccc
    86701 acggacgata aagcttttcg ctcccatcga ccctggaata attccatatt gaccagcacg
    86761 cgcagacacc gcgcctttac gcgtcacgta gatctcttcg ccaaagtgct gttctttttg
    86821 cacatagttg tggtgacagt tgatctcttc catcgccagc gtttgctgtt gtcttaccgt
    86881 tttctgcgta acgctctgca acgctgttac tacgttttcc atcattgcat cgcggttaag
    86941 gctggcaaaa agctgcgccc aggccaccgc tttcaggtaa tcatcaaagt attccgtacc
    87001 ttccataaag tacgccagat cacgcgacgg caacgtttca agctggtcct gcatctcttt
    87061 ttgcgccaga tcgataaagt aagtcccgat ggcgttacca attccgcgtg aaccggagtg
    87121 cagcataatc cacacctgat ccgactcatc aagacagatt tcaataaaat ggttacccgt
    87181 tcccagcgtt cccagatgtt tatagttatt ggtattcagg aaacgcggat atttttgcgt
    87241 taaccactga taaccggctt ccagctcagc ccatttagca tcgacgttaa caggtggatt
    87301 ttcccaggca cctttatcac gtttacaacg gccagtggta cgcccgtgcg gcacggccgt
    87361 ttcaatcgcc tgacgcagct ctgccaggtt ttcaggcagg tcttccgccg ttaacgcggt
    87421 acgcagcgcg ttcattccac agccaatatc cacgcccacc gccgccggaa taatcgcccc
    87481 tttggtcggg atcacgctac caatggtgga acctttaccc aggtgtacat caggcattac
    87541 cgcaatatgt ttgaaaataa acggcatctt cgccgtatta ataagttgct gacgcgcatc
    87601 ggcctctacc ggcacgcctt tggtccacat ttttaccggg gcattttcag tggtcagtaa
    87661 ttcgtaattc attttgtttt tctcttttcg ttgtttgctg tcctgataat tgcaaccgtc
    87721 gtgccagaaa attaaataag cagcttaatt ttttaattca ttgttttaaa aaagattata
    87781 tctttacgtc cgtaaccgga gatttcccgc aaagccaatt taccgataat gaaatatcgt
    87841 cttttataag gatatctaag atgcgtaaaa cagtggcttt tggctttgtc ggtaccgtac
    87901 tggattatgc cgggcgcggc agtcagcgct ggtcaaaatg gcgtccgaca ctctgtttat
    87961 gccagcaaga atcgttggtc atcgatcgac tggaattgtt gcacgacgcc cgctcgcgct
    88021 cgctatttga aacgcttaaa cgcgatatcg ccagcgtttc gccagaaaca gaagtggtga
    88081 gcgttgagat tgaactgcat aacccgtggg atttcgaaga ggtctacgcc tgcctgcatg
    88141 atttcgcccg tggttacgag tttcagccag aaaaagaaga ctatttaatt cacatcacca
    88201 ccggtaccca cgtcgcgcag atttgctggt ttctgctggc agaagcgcgt tacctgcccg
    88261 cccggctgat acaatcttca cctccgcgca aaaaagaaca gccgcgcgac gcatcttcga
    88321 gttaaaacag gcgcggcatc agtttagcgg cgcgtttgtg gaagtgaact gcgccaccct
    88381 gcgcggcgat accgccatgt cgacgctgtt tggtcatgta aaaggcgcgt ttaccggggc
    88441 gcgggaatct cgtgaaggtt tattacgcag cgccaacggc ggaatgttgt ttcttgatga
    88501 gattggcgaa ctgggcgcag acgaacaagc aatgctgcta aaagccattg aagagaaaac
    88561 cttttacccg tttggcagcg atcgccaggt gagcagtgat tttcagctta tcgccggaac
    88621 ggtgcgcgat ttgcgccagc tggttgccga aggcaaattt cgcgaagatc tgtacgcgcg
    88681 gatcaatctc tggaccttca ccctgccggg tctacgccag cgccaggaag atattgaacc
    88741 gaaccttgat tatgaagtgg agcgccacgc cacactcact ggcgacagcg tgcgttttaa
    88801 caccgaagcg cggcgcgcct ggttggcttt tgcgacctct ccccaggcaa catggcgcgg
    88861 taactttcgc gaactttctg ccagcgtcac gcggatggcc acctttgcca ctagcggacg
    88921 catcactctg gacgtggttg aagatgagat aaaccgtctg cgctataact ggcaggagag
    88981 ccgcccctcc gcgcttacgg cgttgctggg cgcagaggca gaaaacatcg acctttttga
    89041 cagactgcaa ctggaacacg tgatagctct ctgtcgccag gcaaagtcgc tttccgctgc
    89101 cggacgcctg ctttttgatg tttcgcgcca gggcaaagcc agcgtcaatg acgcggatcg
    89161 gctacgcaaa tacctggcgc gttttggtct gacgtgggaa gccgtgcagg atcagcacag
    89221 ctccagttga atatgatggt ccgtcagcac ctgcattacg cttactggcg gcggggcgtc
    89281 ggtgtagacg gcatctacca tgctgatgct gcccatattg accatcgcgt tacggccaaa
    89341 tttcgagtga tcgacaacca gcataacgtg gcgcgagttc tcaataatgg cgcgcttggt
    89401 gcgaacttcg tggtaatcga actccagcag cgagccgtcg ctatcgatgc cgcttatccc
    89461 cagaatgccg aaatcaaggc ggaactggga gataaaatcg agcgtcgctt cgccaatgat
    89521 cccgccatcg cggctgcgta attcgccacc ggcgagaata atgcggaaat cttcttttac
    89581 catcaacgtg ttagcaacat tgagattgtt ggtgacaata cgcaaattgc tgtgattaag
    89641 cagcgcgtgc gccaccgctt ccggcgtggt gccgatatcg ataaacagtg tcgagccgtt
    89701 ggggatttgc tccgccactt tgcgggcaat cctctctttt tcttcggtct gggtggcttt
    89761 gcgatcgtgc cacggcgtgt taaccgaact ggaaggcagc gccgcaccgc catggtgacg
    89821 caggatcagg ttttgctccg ccagctcatt gaggtcgcgg cgaatagtct gcgggctgac
    89881 ggagaaatgc tctaccagct cttcagtact gacataaccc tgctgtttaa ccagttcgat
    89941 aataccgttg tgacgttgtg tttgtttcat ttataaatcc ctggaattat tttcgttttc
    90001 gcgcattgag cgaatcaaca aaagccatcg ctaaacccac ggctaacccg gcgatgtgtg
    90061 ctccgttcgc catcgacatc ccaaacaaat caaaccatcc ggcgacaatc cagatcagcg
    90121 caaagataat taacccacgt tgcaggtaaa tgccactttg cggatcgcgt tcaccacgta
    90181 gccagacgta gcccatcagc gcatacacca cgccagaaag cccgccaaac cacggcccgc
    90241 tgaatttttg ctgcacatag ccgcttaaca gggcgctaat gagagtaata acaattagct
    90301 taccgctacc gaggcgtttt tccaccgcac cgccgagata ccaccaccag agcaggttaa
    90361 agaggatatg catcagcgag aagtgcatta acgcgtgggt gaagtaacgc cagaactcaa
    90421 atttcagtgt tggatcgaat ggccaggcca gccataacat cacttcctga tcgccgagaa
    90481 tttgcatggc gataaacacc accacgcagg cgatcatcac cacccaggtt accggacctg
    90541 cgcgttcacg caaggcagca aagaaaggat aacggcgata atgcagacca ctgccggtat
    90601 ggcccgcctg ccagctggcc gccagataac gcggatctgc cgggttttcg agaaaacgcg
    90661 ccagctccgc ccgcacgcgc tcggcctggg actcatccgc cagccagaca tcgctttggt
    90721 tatgttgttg aatcgtaagg ataacaccct gcgtcgccat gtaatcaaca aacgcctgcg
    90781 ccacgcgggg gttagcaaaa gaggtaatca tcaacatcgt tgctgtcgct tattccacac
    90841 aaaaggggac agtataaagc gttacgcgcc gtacgccacc tctgcgggaa actgacgctg
    90901 ccaggcttca aaaccgccgt caatgctata gaccacatcg tagccctgtt gcagcagata
    90961 ctgcgccgcg cctttgctgc tattgccgtg ataacacatc accatcaccg gagtgtcaaa
    91021 gtcgttatca cgcataaacg cgcccagcgt gtcgttggtt aaatggaaag cctgcaccgc
    91081 atgccccatt gcgaaactct gtggatcgcg aatatcgacc agcaccgcct ctttttcctg
    91141 caacttctgg tgcgcgacgg caacgttaat acattcgaac tgatccatgc gtctctcttt
    91201 ctttacaaac aagtgggcaa atttaccgaa cagtttacgt cgaagcggca gataaacgcc
    91261 ataatgttat acatatcact ctaaaatgtt ttttcaatgt tacctaaagc gcgattcttt
    91321 gctaatatgt tcgataacga acatttatga gctttaacga aagtgaatga gggcagcatg
    91381 gaaaccaaag atctgattgt gatagggggc ggcatcaatg gtgctggtat cgcggcagac
    91441 gccgctggac gcggtttatc cgtgctgatg ctggaggcgc aggatctcgc ttgcgcgacc
    91501 tcttccgcca gttcaaaact cattcacggt ggcctgcgct accttgagca ctatgaattc
    91561 cgcctggtca gcgaggcgct ggctgaacgt gaagtgctgc tgaaaatggc tccgcatatc
    91621 gccttcccga tgcgttttcg cctgccacat cgtccgcatc tgcgcccggc gtggatgatt
    91681 cgcattggtc tgtttatgta cgatcatctg ggtaaacgca ccagcctgcc gggatcaact
    91741 ggtttgcgtt ttggcgcaaa ttcagtgtta aaaccggaaa ttaagcgcgg attcgaatat
    91801 tctgactgtt gggtagacga cgcccgtctg gtactcgcca acgcccagat ggtggtgcgt
    91861 aaaggcggcg aagtgcttac tcggactcgc gccacctctg ctcgccgcga aaacggcctg
    91921 tggattgtgg aagcggaaga tatcgatacc ggcaaaaaat atagctggca agcgcgcggc
    91981 ctggttaacg ccaccggccc gtgggtgaaa cagttcttcg acgaggggat gcatctgcct
    92041 tcgccttatg gcattcgcct gatcaaaggc agccatattg tggtgccgcg tgtgcatacc
    92101 cagaagcagg cctacattct gcaaaacgaa gataaacgta ttgtgttcgt gatcccgtgg
    92161 atggacgagt tttccatcat cggcaccacc gatgtggaat acaaaggcga tccgaaagcg
    92221 gtgaagattg aagagagtga aatcaattac ctgctgaaag tgtataacac gcactttaaa
    92281 aagcagttaa gccgtgacga tatcgtctgg acctactccg gtgtgcgtcc gctgtgtgat
    92341 gatgagtccg actcgccgca ggctattacc cgtgattaca cccttgatat ccatgatgaa
    92401 aacggcaaag caccgctgct gtcggtattc ggcggtaagt tgaccaccta ccgcaaactg
    92461 gcggaacatg cgctggaaaa actgacgccg tattatcagg gcattggccc ggcgtggacg
    92521 aaagagagcg tgctgccggg tggtgccatt gaaggcgacc gcgacgatta tgccgctcgc
    92581 ctgcgccgcc gctatccgtt cctgactgaa tcgctggcgc gtcattacgc tcgcacttac
    92641 ggcagcaaca gcgagctgct gctcggcaat gcgggaacgg taagcgatct cggggaagat
    92701 ttcggtcatg agttctacga agcggagctg aaatacctgg tggatcacga atgggtccgc
    92761 cgcgccgacg acgccctgtg gcgtcgcaca aaacaaggca tgtggctaaa tgcggatcaa
    92821 caatctcgtg tgagtcagtg gctggtggag tatacgcagc agaagttgtc gctggcgtcg
    92881 taaggatgat gcccggttgt gaagtgcaac cgggctattt ggattcctta gactattcat
    92941 tgtaaataat tgaataacgg cccctgagct aaaatcgcca aacttaagca cgttacccaa
    93001 tcgccccact tcttcctctg gtttctattt tttctgcacc aatggcaata atatcccaat
    93061 gcaaaaccaa gctgtgcact aatcgggccg acaaaatcag tccaaggctg cggccaaaaa
    93121 ggggaaaaat tcaggggaag ccagacaaac gtcagaaaga aatttcggat attttccagc
    93181 aacgctgatt tctctggaga aatataaaga atgaaattcg agagaattaa ccagaaatag
    93241 accggcgaaa agaccagcca gcaatgcagt ctgtttttta attcaggagg cgttctgcgg
    93301 gagaattgcc aaccgatgcc agccattaca ctgaaaaaaa gtgcatattc aaccattaac
    93361 tcaagttgta gttcctgaag agaaatacgc acaaccagga gcggaaagga gaatattatt
    93421 tctgccagaa aagttaacgt gaatataagt acggctaaga caataaccgt cggccatacc
    93481 aataatggca cgataacaaa accgagaatt acagggggaa tataaagccg gaatttaatc
    93541 atttttattt tcctggtgat tcggatgatg cgtcatacat cgcgcataaa cactcatcaa
    93601 ttatccagtc gtctacccag acctgatgct gtgtgccctg ttctggctgc tttggtgggt
    93661 tctcaatcgc ttgccgcgct ctttctttca gtaaaggatg attatttcta gcccaggcat
    93721 tgatttgggt ggtaatggcg ttatcgatat cgtcctggtt atctctgata taatcctcac
    93781 aatatctgtt caataaacag gcaagagaaa cggtcgacca aaagccggga actgaattaa
    93841 tggcatccag caatggcgca agagcgatat cggcacggaa aaacatttcc agcatatact
    93901 gtaattcgac agcttcatcg ccggataccc aatcagtcat atactggcag gcaaatcgct
    93961 gtaaaattgc ccgttcatcc gcacgccagt tttctttgtt ggcaaggttg atacggcgca
    94021 gagaaaaaac tacgtgaata ttgtcaaaat gcccctgtgc aacaaattcc agtaaacgcg
    94081 gcagaaaata gcggacctca tcgctatttt gcggatcagg ccctgggcta ctattccagg
    94141 cattgattaa ggaatacggg atcgctcgta acgatgtact ccgtaatgcc ttttgttcct
    94201 gctgactcag gcaatattca caacaaacca caaatttctg cggcacccga taacgacgaa
    94261 aggtgtgata aatctgctca attaatgcct gcatggcggg gctgggttgc atagttaccc
    94321 cctcgcccgc tgatcgcgtg taaacggttg atagacaaaa cgagcattcg ccgcgttcag
    94381 gcgaataagg gcgctacgcg aaagggaaaa gctggggcga atgcaactat tgcagtctcg
    94441 acagctttcc tctgcgcaat tttccactga gtaatgacgc gtgtagagtt tcagttcggc
    94501 ctgcttatcg cgaaatccgc gtaaactgtt attgttatga aatttccagg catctttaaa
    94561 gacctgaaag atatagacat cctcaatcca ggagtcacat tttttaagcg ggcgctttgc
    94621 tattatcacc cgctcatccg gatttagcat cgccgtaaat tgtacggtga cgtcatttgt
    94681 cgtgttcagg tcacattgaa catctggtac atcactgata taggggtcaa atccaaaatc
    94741 agcaccacag gcacaaagac ggctaatggt ggcggtatcg agggaaaatc ccggccatac
    94801 cttgctggag gttaagccaa tatttaatgt gacatcatat cgaagcaatc cctcaagacc
    94861 gtcagcaccg acgaactttt ccccttcatc cagggcctgg ttaataagtt gctgaacaat
    94921 gccttcatcc cagtatgccg atttgcagtt ggcaatcgta atatccacgc ctgtgaaggg
    94981 atcagtaccg gcaaggcttg caaataatgt attaactaat tccattttta ttatccagct
    95041 ttaaggtagc gtcttataca tcgggacata acctacatct tccaccaaac tctgtccctg
    95101 cggcgttaaa aaccactcca acaatttttg tgtctctgac gtcgtatttt cccgcgttac
    95161 cataaaggca tcgacgatat agggatattt gccgttgcga atgttttcgg cagtcggtgc
    95221 aataccgtta atcgctagca gttttatatt tttatcgtcg ttcatttgtg ttgcgtaata
    95281 acggaaggtg taacctattg cgttgtttgt attacggtat tcggcaacga ctttaatcat
    95341 tccctccatc atacttgcca cttctgtttc ctgcggcgag atcatgcgga catttttcat
    95401 gacctgggat tgcatcaccg tctggctacc agagtcttcc gggcgttgcc aggtctggat
    95461 ctcctgatcg ttaccgccaa ccgtgcgcca gttggtaatt gcaccactga agatgtcacg
    95521 cacttgttgt tcggtcaggg aattaaccgg gttatctgca ttgacgatga aaacaaacgc
    95581 ttcacgggca aaaggcgtgt aaatcaaagt gacgcccgat tcctccgcgc gttttttctg
    95641 cccaccggaa ggttgagcca caaagataat atcggcatta cctttaacaa tcttgttata
    95701 tgcttcggga gtacgagagt tcgccagata ttcccattcg tgaaaatctt ccggcaaaac
    95761 gcttagtgca taaaaggcag aggcataaat ggggtacgcc gccgttgcgc catcaagtcg
    95821 cggccagttt tgcgtgaact gaatttgcgg tttaccacgc agtggtgtca gttgattatt
    95881 gagtttatcg ggtcgccagg cccaggtatc aatattttca cgcacaccga cgccgggatt
    95941 aagctgttta tataaataag cctggtaacc ggcgacaaaa ccaagcatga gaaggataaa
    96001 caagagagaa ttacggcaac gtaacccgga cgtgcccgtt aaaggatatc tggtcggcca
    96061 gccgtacccc agcgaaaaac tgatatgcgc gccaacaggt ataatgacta accaccagta
    96121 ctcaccaccg actaatgagg ctaacaaaac gcccagccag gggagcataa gatcagccaa
    96181 ccaacccgct cccatctggt gaaattgata atctgccaga tgcagcgcga ttatccagac
    96241 ccccagggtt aataatgcgg gtactaaaaa aagacattgt cgccagacgg ctatttctct
    96301 tttttctggt gcactatcgt ccgcccacac tcgccccgag aaaaatagca ttaatgcgcc
    96361 cagcgccagc ataaaaatgt tgagcagaag gtaaattgtt ggcgtgataa agatattaca
    96421 aacttcaata ataccggcaa gtccgacacc cagcatggca ataaccgccg ccaaaaactg
    96481 cgccagaatg gggatgccga aaaaagtcat taccagcgag gtcaaaatcc attttctgtt
    96541 ttgcattatt ctttccattc tttctgaatg gcgaaattat actccccgag tccccttgcc
    96601 ccttctggac acttttccga aatgatggcg aaaaaaacgg gaccctttgg ccccgttcta
    96661 tttattagtg aaattacaat ctcaccggat caatatgcca gatatgatcg gcgtactctt
    96721 tgatagtacg gtcagaagag aagtagccca tattggcaat gttcagcatc gcttttgcgg
    96781 tccactcttc ctgaagctcg tagagttcgt cgactttatc ctgacaatcg acatagctgc
    96841 gataatccgc cagaacctga tagtgatcgc cgaagttgat cagcgaatca accagatcgc
    96901 gatagcgacc cggatcttcc ggactgaata caccgctgcc gatttgcgtc agcacctgat
    96961 gcagctcctc atctttctcg tagtattcac gcggtttgta gccctgacga cgcagttctt
    97021 ccacttcttc cgctgtgtta ccaaagataa agatattgtc agcaccgaca tgatccagca
    97081 tctcgacatt cgcaccgtcc aacgtaccga tagtcagcgc accgttaagc gcaaacttca
    97141 tgttactggt gccggaagct tccgtccccg ccagcgaaat ctgttcagac agatcggccg
    97201 ccggaatgat caactgcgcc aggctaacgc tgtagttcgg gatgaacacg actttcagct
    97261 tgtcgccaat ctgcggatcg ttgttgatca ctttcgctac gtcattgatc aaatgaataa
    97321 tgtgcttcgc catgtaatag gccgaagccg ccttaccgcc aaaaatattc acgcgcggta
    97381 cccacttcgc atccgggtcg gccttgatgc ggttatagcg ggtaatcaca tgcaacacat
    97441 tcatcaattg acgtttgtat tcgtgaatac gtttgatttg tacatcgaac aacgcctttg
    97501 gattcaccac cacattcagc tgctgggcga tatactctgc cagacgcttt ttgttctcca
    97561 gcttcgcctg atgcacagcg tgattaacca ttgggaaatc acagtgttgt tgcagctcat
    97621 taagcaggct aaggtcggtg cgccagttac ggcccaggtg ttcgtccagc acggctgaaa
    97681 gcgatgggtt cgctaccgct agccagcgac gcggcgtcac accgttggtg acgttggtga
    97741 aacgacccgg gaagattttc gcaaagtcgg caaacaacga ttgcaccatc agattagagt
    97801 gcagttccga tacaccgtta actttgtggc tcacaacaac cgccagccag gccatacgca
    97861 cacgacgacc gttggattca tcaatgatcg acgcccgtcc cagcagatcg gtatcgttcg
    97921 gatactgttc ctgcaaggtt ttcaggaaat agtcgttgat ttcaaagatg atctgcaggt
    97981 gacgcggcag aattttaccc agcatatcaa ccggccaggt ttccagcgcc tcgctcatca
    98041 gcgtgtggtt agtgtaggag aagacctgac aacacacctc aaacgcgtcg tcccagctaa
    98101 attggtgctc atcgatcagc agacgcatca tctcaggaat cgacagtacc ggatgggtat
    98161 cattgagatg aatcgcgatt ttatccgcca ggttatcgta ggttttatgc aactgataat
    98221 ggcggcttaa aatgtcctga atggtcgagg aaaccaggaa gtattcctga cgcaggcgca
    98281 gctcacgccc ggagtaggtg gagtcatccg gatacagtac gcgagatacg ttctcggagt
    98341 ggtttttatc ttccactgcc gcgaagtagt caccctggtt gaatttaccg aggttaattt
    98401 cgctactggc ttgcgcactc cacaaacgca acgtgttggt cgcgtcggta tcgtaaccag
    98461 ggattatctg atcgtaagcg actcccagaa tctcttcggt ttcaatccag cgcgtttttt
    98521 taccttcctg ctgaatgcga ccaccaaaac ggactttata gcgcgtgttg tggcgtttga
    98581 attcccacgg gttaccgtat tccagccagt agtctggcga ctctttctgg ctaccgttaa
    98641 cgatgttctg cttgaacata ccgtagtcat agcggatgcc gtaaccgcgc cccggcaacc
    98701 ctaacgtcgc cagagaatca aggaagcaag ccgccagacg tcccaggcca ccgttaccga
    98761 ggcccggatc attttcttca tcaatcagct cttcgagatt taaccccatc gcttccagtg
    98821 cgccctgtac atcttcgtaa attcctagcg acaacatggc gttggagagc gtacggccaa
    98881 tcaaaaactc catcgacagg tagtaaacct gacgagtttc ttgcgacaac tgggcacggt
    98941 ttgaacgtaa ccagcgctcc acgagacgat cgcgcacagc aaataacgtt gcgttcagcc
    99001 attcatgttt attggcgacg accgggtcct ttccaatcgt aaacatcagc ttgtaagcga
    99061 tagagtgctt aagagcttct acgctaagcg tgggcgatga atatgtaaac ggagcattca
    99121 tataggcgtt tcctgaaaac tatttcaagc gatagtaaag ctcacggtac gacttcgccg
    99181 cgacctgcca gctaaaatcc attgccatag cctgacgttg cacaaaccgc cacagtgaag
    99241 gacgggacca cagtacaaaa gcacgccgaa tagcccgtaa cagcgaccag gcattactat
    99301 cttcaaagac aaacccactg gcgacgccat ctgcaaggtt ctcaagagaa cagtcagaaa
    99361 ccgtatcagc aagcccaccg gtgcgccgca ctaacggcag cgtaccgtac ttcaatccat
    99421 aaagttgcgt taagccgcac ggttcaaaac ggctgggcac cagaatgacg tccgcgccgc
    99481 ccataatgcg atgcgaaaat gcttcgtgat agccaatctg aacgcccacc tgaccggggt
    99541 attccgctgc cgccgcaagg aaaccttcct gcagcaccgg atcgcccgcg ccgagtagcg
    99601 ccagctgccc gccctgctcc agaagacccg gtaaggcttc cagcaccagg tcgagacctt
    99661 tctggctggt cagacggctc accactgcaa aaagcggcac tttatcgtca accttaagcc
    99721 ccattgcgat ttgtaactgg cgcttatttt ccgctttatc ttccaacgta tcgcgggtgt
    99781 aacgcgaggc caacagtaag tccgtctctg gactccagat tttctcgtcc acgccgttca
    99841 gtacgccgga aagacgccct tcacggtgac gctgttgcaa cagaccttcc ataccgtagg
    99901 caaactgcgg ttcggtgatc tcgcgagcgt aggttggact gaccgccgta atgtgatcgg
    99961 catagtacag accggccttc aggaaagaga tttgtccgtt gaattccagc ccatgaatat
   100021 taaagaatga ccatggcaat tggatgtcat tcatatgatg tgcataaaac atgccttgat
   100081 aggccaggtt gtgcacagta aacaccgact tcgccggacg cccgcgcgcc gccagatacg
   100141 caggcgcaag gcctgcatgc cagtcgtgcg catgcaccac atcaggacgc cagaatgggt
   100201 caagcccgct ggccatttct gccccaaccc accccagcag cgcaaaacgc aatacgttgt
   100261 cggtataggc aaataagttg gtatcgtgat acgggcttcc cggacgatca tagagatgcg
   100321 gcgcgtcaat caggtatatg ccaaccccgt tgtaatgacc gaacaacagc gtgatatgtc
   100381 cggcgaaggt atcacgacgg gatactacct gcgcatcggt aacgccacgg cgaatatcgg
   100441 gaaatgcagg caacagtacg cgagcgtcaa cgccgtctgc gatttgtgct gcgggtaatg
   100501 ccccaataac atcagccaga ccgccggttt taagcagcgg gaacatctct gaacatacat
   100561 gtaaaacctg cattatcgct cctgtttatg ccctaacttc cgtagcattt cgcgcgttac
   100621 cagcacgatg ccttcttctg aacgatagaa acgacgtgca tcttcctctg cgttttcacc
   100681 aatcaccatg ccttccggaa taacacaagc acgatcgatg acgcagcggc gcagacggca
   100741 cgagcgacct acccatactt ccggtaacaa tacggcggaa tcaatgttgc agaatgaatt
   100801 cacgcgaacg cgcgagaaca gaacggactg caccaccacc gaaccggaga tcacacagcc
   100861 gccggatacc agtgagttaa gagtcatccc gtggctaccg gagcgatcct gcacgaattt
   100921 cgctggcggt aatgattcat tgtaggtgcg aattggccaa ttgcgatcgt acatatccag
   100981 ttccggcacc acagaggcca gatcgaggtt cgctttccag taagcttcca gcgtacccac
   101041 atcgcgccag tacggctcgg catccgggtc ggattgtacg caagagagcg ggaacgggtg
   101101 cgcataggcc agaccggctt cggtgatctt gggaatcaaa tctttgccaa agtcgtggct
   101161 ggagttctca tcacgatcgt cttcttccag cagttcatac agatagtcgg cgtcaaagac
   101221 gtagataccc atactcgcca gagatttgct cggatcgttc ggcattgacg gtgggttagc
   101281 aggtttttca acgaattcga taattttatc gttctcatca accgccataa cgccaaatgc
   101341 ggaggcttct tcaatcggta ctggcataca agcaacggtg caacgtgcgc ctttttcgac
   101401 gtgatcgata agcatacgcg agtagtcttg cttgtagata tggtcgcccg ccaggatcac
   101461 cacgtattcc gctttataac ggcggataat gtcgaggttt tgggtgaccg catctgcggt
   101521 gccgcgatac cagttttccc ctttcattct ctgctgtgct ggcagcagat cgacaaactc
   101581 gttcatttct tcattgaaga atgaccagcc gcgctgaatg tgctgcacca gagtgtggga
   101641 ctggtactgg gtgatcacgc ccatacgacg gatcccggag ttgatgcagt tagacagcgc
   101701 aaagtcgata atgcggaact taccgccgaa gtgtacggcc ggttttgctc gcttattggt
   101761 taaatccttc aggcgggtac cacgtcctcc cgccagtatc agggcaacag atttcaatgg
   101821 cagctggcgc gccaacatta agtgatcgtt cttctctaaa ctaaccatga ctaactcctt
   101881 ttttatcatc tctggaacac acacaatccg tgtgcaggtc cctgccagac agccgtaatc
   101941 actgggttat cctctccagc gaatggggga atggcgtgcc actccccagc aggtaaaaca
   102001 atctctgtta cctcaagcgt ggcgttaatt gcgatcaaaa agcgatccga gagcagaatt
   102061 tgcagctgtt tcggcccgtt ttgccactca tccgtgctta aaggttgagc atatcgattt
   102121 agccaacgga cattgccgtc gccttcttcc caccagcgat tctccaccaa agcgggaatg
   102181 cgcttgcgca gatggattaa cgcggcggta aatgcggtta aaccactgct tgcctgcgac
   102241 cagtccaacc aggttaattg gttatcctga cagtaggcat tgttattgcc atgctggctg
   102301 tgaccatgtt cgtcaccggc cagtaacatc ggcgtaccct gggagagcaa caacgttgtt
   102361 aacagggcgt gaatgctgtc gcgccgccgt tcaaccaggt caagagaacc gcctaaccct
   102421 tctttaccat gattgttact gtaattgttg ttggtcccgt cgcgattttc ttctccgttt
   102481 gcttcattgt gtttatggtt gaagcaaacg cagtcgcgaa gcgtaaaacc gtcatgcgcg
   102541 gtgacgagat taatcgcggc actcggcaga cgaccattac gtttaaaaac atcgctggag
   102601 gcagcaaaac gcccggcaaa cgcccccaga ggcaaatcat aatgtagcca gaaacgacgg
   102661 gcagcatcgc ggaaatgatc gttccactcg gcaaacagcg gcgggaaatt tcccacctga
   102721 taaccaccag gagcgatatc ccacggttca gcaattaact tcacctgcga gagcaccggg
   102781 cagttctgga tagcggtaaa caacggcgca tcctgacgga actctggcgt acggcccatg
   102841 actgccgcca gatcaaagcg gaaaccatcg acgtggcagg tttctaccca ataacgcagg
   102901 caggcgctgg cataatccac caccgccgga tgactcaaat tgagcgtgtt gccgcaaccg
   102961 gtccagttgt gataatcgcc gtcttctctt atccaataat agctacggtt atcgatccca
   103021 cgcagcgaga ataacgggcc gtcgaggtcc agttccgcac tatggttgag cacgatatca
   103081 agaatgactt cgatacccgc tttatgcagt gctttgattg catcgcgaaa ctcatccagc
   103141 gccgtttctg gcgagcaggc atacgccgga tgcagcgcaa acatcgccac cgggttgtaa
   103201 ccccagtagt tacttagccc catgcgttgc agacgtggtt cactggcaaa ctgcgccact
   103261 ggcagcagtt ccagcgcggt aatgcccaat tgtttcaaat agttgatcat caccggatgc
   103321 ccgagggctt tataagtgcc acggatctcg accgggatct ccgggtgcaa gtacgttaat
   103381 cctttgacat gggcttcata aatgatggtg ctgccccacg gcgtgcgcgg cggggcatca
   103441 tcttcccagt catagtgatc aaccaccact acgcatttcg gcgcaatggc ggcgttgtcg
   103501 cgatagtcag gttcattatg accggcgtgc agcagcgggt tatctttaaa ctccccctca
   103561 atttgccgcg cgcaaggatc aatcaacaac ttcgccgggt taaagcgatg cccctcggcg
   103621 ggttgccagg ggccatgaac gcgataacca taacgcaaac ccgggcgcgc atccggcaga
   103681 taaccgtgcc aaatgtcgcc actgtgccct ggcaagtcat agcgatgttc ctggccattg
   103741 gcgtcaaaga cacacagttc tacccgctcg gcatgagcgg agaaaagtgt gaagttgacg
   103801 ccctgaccgt cgtaatgcgc gccgagggga gcgggtttgc caatggcgag ttgtgtcatt
   103861 ctgcctcccg aaccagccag atagtggcca gcggtggtag cgtcaggctt agtgaatgct
   103921 gacgaccgtg gctggcaatc tcatcgctgt gtaccgtgcc gccattgcct gcattactgc
   103981 cgtgatagtg catggaatcg gtattgagga tttcacgcca tttgcccggc tggtttatgc
   104041 cgaagcgata atcatgacgc ggcaccggcg taaagttact ggcaacgatg atttcgttac
   104101 cctctttatc gcgacgcaca aagatcagca ccgagcgttc tttgtcatcc accaccagcc
   104161 attcaaagcc gtacgggtca aaatccagtt catgcattgc tttatggtgg cggtaggtga
   104221 ggttcagatc gcgcaccaga cgctggacac cgtggtgcca gttatcgccg ccttccaaca
   104281 gatgccagtc gaggctggcg tcatggttcc actcgcggcc ctgggcaaat tcgttaccca
   104341 tgaacagtag tttcttgccc gggaatgccc acatccagcc atagtaggcg cgcaggttcg
   104401 cgaatttctg ccatgcgtcg cccggcatgc ggtcgagaat cgatttttta ccgtggacca
   104461 cttcatcatg cgacaacggc aggacgaagt tttcagtgta gttgtagaga atcccgaagg
   104521 tcagtttatc gtgatgatac tgacgataaa ccgggtcgag cttcatgtag tccagggtgt
   104581 catgcatcca gccgaggttc cacttgtacc agaagcccag accgcccata tcctgcggac
   104641 gagaaacgcc agggaaatcg gtagactcct cagccattgt caccgcaccg gaaacctgct
   104701 caccaagaat acggttggta ttacgcaaga attcaatcgc ttcaagattc tcgcgcccgc
   104761 caaattcgtt cgggatccac tccccctctt tacggctgta gtcgcgataa atcattgacg
   104821 ccaccgcatc gacgcgcagc gcatcaatac caaaacgttc aatccagtaa agcgcgttac
   104881 cgacgaggaa gttactgact tcacggcgac catagttgta gatcagcgtg ttccagtcct
   104941 gatgatagcc ttcacgcgga tcgctgtgtt catacaagtt cgtgccatca aattcggcaa
   105001 gcgcaaagtc atcagtcggg aagtggcctg gcacccagtc gagaatcacg ttcagaccag
   105061 ctgcgtgtgc ggcatcaatg aaataacgga agtcgtcgcg agtaccaaaa cggcgggttg
   105121 gtgcatacag gccggttggc tgataacccc aactgccatc gaagggatgc tcgttaatgg
   105181 gcagtagttc gaggtgggta aagcccatcc atttagcata aggcaccagt tgatcggcca
   105241 gctcgcggta gctcaaccag aaattgttgt cggtgtggcg acgccaggaa cccaggtgaa
   105301 cttcataaat agagattggc gcatcaaact gattcgcttt tttgcgctct tcagtctgta
   105361 caaccttttc cggcagcccg caaataagag acgcggtttc cgggcgcatt tgcgcttcga
   105421 aggcataagg gtcggacttc agacgcaagt tgccattggc atcaatcatc tcgtatttat
   105481 agagctgacc gttatgcgcc ccagggataa acagttccca gatgccgctc tctttacgca
   105541 ggcgcatcgg gtgacggcga ccgtcccagt agttgaattg cccaaccacc gagacccgac
   105601 gggcgtttgg agcccagaca gagaaacgcg tacctgtgac gccatccata gtatctgcat
   105661 gcgcgcctaa ggtttcatac gggcgcaggt gagtaccttc agataatagc caggcatcca
   105721 tttcctggat tagcggacca aaacggtaag gatcatcaat caggttttgc tgaccatgcc
   105781 agacaacagc caactgatag cggaaaaaat tcttacgtcg cggaatgacg ccgctaaaga
   105841 atccccgtga gtcgagacac tccagttttg cgagtttgcg cccggttttc ggttcaatca
   105901 cccacacatc ggtagcgtcg ggtaaaaggg cacggacttc cagtcccgcg gtggttttat
   105961 gcattcccag tacggaaaaa ggatccgcaa aatggcctgc aattagcgcg ttaatcacgt
   106021 ctctatcgat acgatcggac atgcttgtct tcctgtttta ttgtgtcacc ccatccaatt
   106081 ggatttttga cttctggttg tgacattttt ttgacctgaa cggcgcagca ctctgtgcat
   106141 cctctctgcg tcgtcctcac ttcaggtaag gctgtgaata ctcatgtatt cagccaccct
   106201 taaagaatag ccaatgctct atttaactcc cggtaaatca tgaaacatct gcgcttactc
   106261 ctgtattacg cactaacagg ggcggcatcg cgccccagat ttaatgaata aagattacgc
   106321 cagttgacga agcatccgac gcagcggctc cgcggccccc cacagcagct ggtcgcccac
   106381 ggtaaaggct gacaggaact ctggtcccat attcagctta cgcaggcggc ctaccggcgt
   106441 ggtcagcgtg ccggtaacgg cagctggggt tagctcacgc atagtgattt cccgatcgtt
   106501 cggaacgact ttcgcccacg gattgtgcgc agccagcagt tcttccacgg tcggaataga
   106561 cacatctttt ttcaatttaa tagtgaatgc ctggctgtgg cagcgcaatg ccccgacacg
   106621 cacacataaa ccatctaccg gaattacgga agatgtgttg aggatcttgt tggtttccgc
   106681 ctgccctttc cactcttcgc ggctctgacc gttatcgagc tgtttgtcga tccacggaat
   106741 caggctaccc gccagcggca cgccaaagtt atccaccggc agctcaccgc tacgggttaa
   106801 ggttgtgact ttgcgttcga tatcgagaat agcagaggac ggggtcgcga gttcatctgc
   106861 cacatggcca tacagatggc ccatctgggt taataactca cgcatatgtc gcgcaccacc
   106921 gccggaagcg gcctggtagg ttgcaacgga cacccaatca acaagatcat tggcgaataa
   106981 accacccaac gacatcaaca tcaggcttac ggtacagtta ccgccaacaa aagtcctgat
   107041 gccattattt aatccgtcgg taatgacgtc ctgattgacg gggtcaagaa tgatgatggc
   107101 gtcatctttc atgcgcagag acgatgctgc gtcaatccag taaccttgcc atccgctttc
   107161 acgaagcttt ggatagattt cgttggtata atcgccgccc tgacaggtca caatgatatc
   107221 gagggccttt agcgcctcca gatcaaaggc atcctgaagt gtgccagtgg ttccgccaaa
   107281 agacggcgca gcctggccaa gctgagaagt agaaaagaag acagggcgaa tggcgtcgaa
   107341 gtcgcgctct tcaaccatgc gttgcatgag aacggagccg accataccgc gccagccgat
   107401 aaaaccaaca tttttcataa gcgttttttt cctgcaaaga tgtgtgctgt ataaatgtgc
   107461 cggtctcctc ttggcacatc tttcaccata caaaaagcag ccaaagtcgc aagtgaaatt
   107521 aatcaatgat agcgaagcca tcagtaatgc gacttatcct gctttgttag cacgcagaaa
   107581 gtccgcggca attatcaggg aatttgagtt atgaatgaaa tcatttctgc agcagtttta
   107641 ttgatcctga ttatggatcc gctcggaaac ctacctattt tcatgtccgt actgaaacat
   107701 actgaaccga aaagacggcg ggcaatcatg gtgcgagagt tgcttattgc tctcctggtg
   107761 atgctggtgt tcctgtttgc gggtgagaaa attctggcat ttctcagcct gcgagcagaa
   107821 accgtctcca tttctggcgg catcattctg tttctaattg ccattaagat gattttcccc
   107881 agcgcttcag gaaatagcag cgggcttccg gcaggtgaag agccatttat cgtgccgttg
   107941 gcaattccgt tagtcgccgg gccgactatt ctcgccacgc tgatgttgtt gtctcatcag
   108001 tacccgaatc agatggggca tctggtgatt gctctgctgc tggcctgggg cggcaccttt
   108061 gtcatcctgc tacagtcttc gctattttta cgtctgctgg gcgagaaagg ggtgaacgca
   108121 cttgaacgcc tgatgggatt gattctggtg atgatggcaa cccagatgtt cctcgacggc
   108181 attcgaatgt ggatgaaggg gtaaggttca aacaggtgct gcacacaacc agacgcggca
   108241 cctgttttat tacctgaact gataatgacc ttcgcccaat cggataaccc ttccctcttc
   108301 ttctgcgcgg ttcagccaca cactaatttg ttcgatggtt aaaccagtac aagacgacag
   108361 cctttcaata cttatcgatt cctttgcaaa gatcgccagt tccgccacaa agagttgata
   108421 aaaatcgaca ggcgtatgcg cgggcattac tgcgtaattt gcgtcatctt catgcagcga
   108481 aaacagttct ggttgagcgt tacggagtgc tctgtcatac atcagcccag gaacgtctga
   108541 cagtctgatc ggcacattct cagcctgttc agcactccat gacgccccat tttctactaa
   108601 tcgtgaattg gctgattgca tatcctgatt tggtttaacc tgcacaggca gccactgatg
   108661 ttttaatgcc tctatcgccc cggttatcgt tccaccggtc attcccgcac gtacaaccat
   108721 tgcgctttca gcaaggcaat aaatatattt atttcgcgcc atcgcattgc cgacggtgaa
   108781 acgcacctct gggtaaaacg gcgaaatcaa caccaggttg cctgctataa gcccttcacg
   108841 ccatttcact aacgtactcg tttttaacaa gctatcagct aatacgccaa cggcagttcc
   108901 cccggcctcc agtgccgacg ccattgcaca ttcatcaata cctcgcgcac caccagagat
   108961 aacgcaaatc ccctgttggg ccagtttcgc ggccagttgt tgggtatagc gcaaatcgtc
   109021 agtcggagca tctcgcgagc caacaatcgc cataccttcc gcttgcagta atgctttatt
   109081 cccgcagcca aataaaacgg gaggtgcatc cgttcgcaat cggtttttca agcgaacggg
   109141 ataatcagca tctcctcggg ttatgatcca taaaccggcg cgttgccact tatctaccgc
   109201 caacgccaga ctatgaccgc gcgccagcaa gccaagaata cgctcctgag aaatacgcgg
   109261 atcgctccag cctgtaagtt tctcttgcgg ttgcggcacc agaagctcgg cgggactgat
   109321 acgttggtgc ttcagccata atgcaaactt cccccattca ctattactaa gaggtttctg
   109381 ctcactcgcc gccgcgcgag aaaaatcgct ggttagcagg agagtcgctt gtgcattggc
   109441 tgaaagattc atcaattttt taccgaggta gaagcaaggg caagaggata aaccgtcggg
   109501 caacctgcct ggcgtaacag tgctgtcaga actgtcagcg tccatgcaga atccacgata
   109561 tcgtcaacca gcaacgccgg acctggcatc aaaggagggg taatcacaaa cgccccgtcg
   109621 agattttgac actggtgaaa acggttttgc tgcattttct gcggtggatt gtccacgact
   109681 ttttcaatgg catcaataaa aggtaagcca agtttcgccg ccagtcgccg ggcaaaatca
   109741 ggaaccaggt cgaggtgcct taatgaagga acgcagcata cccaggttgg ttcaggatgc
   109801 ggattccagc gttggcgaac catttccgca catgcctcca ccagctcatc actgaatcgc
   109861 cccgtttttt tctcctgtgc tacctgcttt ccccatccgg aatctcccca acgagaaagg
   109921 actcgccccg tagatccttg taaacccgca ggcaagttcc ctttaaatcc gtattgcgta
   109981 aaagctccag atgctacctg tttattgaga tttaatggca ggtcagcgtg tttaataaat
   110041 aaatttgccg catggagtaa gccgctatca atatcgggcg acaacaacgg atgctgaaga
   110101 caactgctac atttaccaca acgttcggca cttggctcat cgagtgcgcg gcgcagaaat
   110161 tgcattttgc actccttggt cgtgatatag ctttcaacat ctgccagctc attttttctc
   110221 tgattcatca aatggttaat tcgttcatgg tcaggagaga aactgacgat agttctgcgc
   110281 cataatttct cggtatacac cacaggcgat ggattttcag ctaccaataa tttcagtgct
   110341 ttttctattt gcccataacg gagattcgtc cgttgttcaa tgcctcgtag ggtaagaccg
   110401 tcattctcgc taagtacgtt gagtatttca tgaatttgcg cctccgcagg aaaggcgctt
   110461 tcacggaaga atttatgaat agcgcggtct tcaccaccac aaagcaatat gccaacagcg
   110521 gaatctatag cacgcccggc acgccctacc tgttggtagt aaccgacaat agaccccggc
   110581 atctggtaat gaataacaaa ccccaaatcc ggtttgtcaa agcccattcc cagcgccgtc
   110641 gtcgcgacca gaactttaat tttatttgcc agcaacgcct gctccagata ttccctggca
   110701 gtattagaat cttccgcgcc ttcacaagtc acgccgctgt agtaagcaaa tgcactgata
   110761 ccatttgtcc ttaaccactc ggcgacaagg tcggcatcgc gagtagtcag ggtataaaca
   110821 attcccgatt tggaaaactg agggataacc gttgctaacc atgccagacg ggatgactgt
   110881 tcacctaaga ctaaggcatc aagcgccaaa ctttcgcgag ccagcgttcc acgctgaatc
   110941 acaatgtcac ccaattgctg acggatatcc tcaacgacac ggttattcgc tgtcgcggtt
   111001 gtacccagaa ctggggtatt cgcaggcagt tggcgcaaaa tatctaatat acgtcggtaa
   111061 tccgggcgaa aatcgtggcc ccaatcagag atgcagtgcg cctcgtcgac cacaagcaag
   111121 ccaatacgat cggctatagg atataaaacc gtttcgataa agtcttggtt tgccaaacgt
   111181 tccggggaaa ccagcaagca atcgacctca ccttgcagca acttatcgct aatacgctgc
   111241 cattcttccc tgtttgtaga attcaacgtt tcagccgtaa tacctaaccg ttcagcggcg
   111301 gcaacctggt tacgcatcag ggcaagcaaa ggagagatga taattgtcgg gccagcaccg
   111361 cgatcgcgga agattttact ggcgatgaag tacacggcac ttttccccca gccagtgcgt
   111421 tggacaacca gcagttttcg tcgttggttc accaccgcgt ctatggcttc ccattgccct
   111481 tccctgaaag tagcggtggt atttcctaac atccgctgta aaagcaattc agctgcgtgc
   111541 ttttccatct ttatctcctg cgatctttag gcagtgatta gaacggatta ttagcaggta
   111601 aatttgcgag agggatcaaa aagatgaaga gaggagataa gagaaatgaa ttactgcgag
   111661 caagctcgca gtaatttgtc gcagtaatta actcaacagc tggaacgcaa tcatcccaac
   111721 gatggcaccg acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
   111781 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaaccg gcgtcgttaa cgtggctgac
   111841 aacaatcgaa ccaccagcga tacaaatcga cagcgccgcc atttgcgcac cggagtagtt
   111901 cagttgttca ataaccggca tcaccagtcc taccgccgtt aaacaggcta cggtggcaga
   111961 accctgaatg atgcgcactg cagcggccag cacgaagcag gtgatggcta tcggcaggcc
   112021 catgccagtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
   112081 gaacacgcca cctgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
   112141 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
   112201 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
   112261 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
   112321 cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttgcctt cgccgagatg
   112381 cggttcgctg atgtcgtcag gaatatgcaa ctccacgtag cggctgatga aattacccca
   112441 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
   112501 gccaaaatcg gcgttcatct gcgatgccag cagcattggc gctggccctg gcaccaggaa
   112561 cgccgccgct gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
   112621 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
   112681 cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc ggctgtgacc
   112741 gaaggattta agcattttga cggcaatctg atcgactgcg ccggtttcat gtaagatctt
   112801 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
   112861 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
   112921 ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
   112981 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
   113041 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
   113101 caccagtaca tcagtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
   113161 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cggctttcaa tcacgtcaaa
   113221 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
   113281 gtgttttttc aatgcagaac agacgatcag tgacacttta ttagtgcgct gcatagcaaa
   113341 cgcggcgtcg ttcagcgcct gcaaccacgg tttgcgatcg tcgtcattca gcggttcgcc
   113401 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
   113461 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcctg agacgcccat
   113521 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
   113581 gaattaaaaa tgttacgggt aactgttatc ggtaacattg tccggccgga caatatcaga
   113641 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
   113701 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
   113761 gccacgaata cgcgccagca ggcgttcagc gccgatactg cccatccgct cacgcggcgt
   113821 cagcacgctc gccagtcgtg gctccatcac ctgaccaatg tcatgaccgt ggaaaccggc
   113881 aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg ccgcgccgac
   113941 cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
   114001 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatacgg
   114061 caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
   114121 gtcgagacgt gcgccgagat aggcaatgtg gcgatgcccg cgcgcaataa tggcggtagt
   114181 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactg
   114241 gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
   114301 cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggacagca tggattcgag
   114361 acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatcgtct gataaccgtg
   114421 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
   114481 aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg gcgcacgatt
   114541 gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
   114601 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
   114661 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
   114721 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
   114781 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
   114841 gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
   114901 aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
   114961 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
   115021 tcacgccgtt aatggtgaca ttgcctttca ccacctggat ccagacgcgg cgttcagcgg
   115081 caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt tccatatcct
   115141 gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt ttgccctgca
   115201 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca ggcatgatcc
   115261 agatctgata cagatgcaga cgctcggtgc tgtttgggtt gtactctgag tgacgaatac
   115321 ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta ttgcccatgc
   115381 tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
   115441 ggtgagtgcc gaagccctgc cctgcttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
   115501 agaagcccat aaagttcgga tcgtaatagt tggcaaaaga gaaagtatgc caggagtcca
   115561 gccagccatg atttgcatga ccacgttcat ttgctttgcg gaagtagatc atttgtattc
   115621 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
   115681 tgaaaattga cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttactt
   115741 agcgagagtt actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
   115801 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
   115861 cgcatcataa acgcgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
   115921 gccctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc
   115981 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
   116041 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
   116101 tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
   116161 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
   116221 aataatctgg tccatcgtat gcacaccaag gccataaaac gcgccatcct gcggcagccc
   116281 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt caacaatctc
   116341 tcccaacttg ccactttcaa tcgctttttt cgctgtcagg aagcaggagt caaagcgacg
   116401 attctgatac ggcgtgacgg tcagcccttt gcttttcgcc aacgcaaaca gctccttcgc
   116461 ctgcgcaagt gtcggagtga acggtttttc gaccagcaca tttttcccgg cttccagcgc
   116521 gcgtttcgcg tactcgaaat ggctgtccgc gtgggtgcag acaacaacca gcttaacatc
   116581 gggatcgttt agtacttcgt cgagatcgct ggtgaaatgg atatgggaat aaatgggagc
   116641 ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc tatccttgcg
   116701 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
   116761 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
   116821 cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata aattgagaac
   116881 gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag caatgtgagc
   116941 aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa taataatcat
   117001 tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg catttgaaag
   117061 tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt gagatagtaa
   117121 tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc cagccggagg
   117181 tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag gagcgactcg
   117241 ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc gtgggccatc
   117301 tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg
   117361 tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa
   117421 tgtgcgacaa ctggttgcgg gtagatcgca ttgaactaac cgtgtttgtc gataacgccc
   117481 cggcaattaa ggtctataaa aaatacggct ttgaaattga agggactggt aagaagtacg
   117541 cattgcgtaa tggtgaatat gtcgatgcat attatatggc gcgggtgaag taagatagtg
   117601 ccctttttct gagatggaaa aagggtgtca ttcaaaatcg acataccttc ctttaaggta
   117661 tttatttgcc caatacataa attgagattt aactcataat tacttcctaa agtgtaatat
   117721 tttatttttt aatatatacg cctacaattt cctggagtaa ataaataaca attaacaagc
   117781 ataatattgc cattgataaa atagcatgcc ataaaaggac ttttcaggga tgagtaatat
   117841 tgtttacctg acagtaacgg gagaacaaca aggaagcatc tccgcaggtt gtgggacttc
   117901 tgagtctaca ggtaatcgtt ggcagtgcgg gcatgaggat gaaatattta cattctcact
   117961 cttaaat
//
LOCUS       DAZGXZ010000003        95662 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000003 DAZGXZ010000000
VERSION     DAZGXZ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95662
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.003"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            45..563
                     /gene="yfgH"
                     /locus_tag="ACHKJF_000239"
     CDS             45..563
                     /gene="yfgH"
                     /locus_tag="ACHKJF_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0947665.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            579..1118
                     /gene="yfgI"
                     /locus_tag="ACHKJF_000240"
     CDS             579..1118
                     /gene="yfgI"
                     /locus_tag="ACHKJF_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP0947666.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1213..2790)
                     /gene="guaA"
                     /locus_tag="ACHKJF_000241"
     CDS             complement(1213..2790)
                     /gene="guaA"
                     /locus_tag="ACHKJF_000241"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP0947667.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2859..4325)
                     /gene="guaB"
                     /locus_tag="ACHKJF_000242"
     CDS             complement(2859..4325)
                     /gene="guaB"
                     /locus_tag="ACHKJF_000242"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP0947668.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4487..5857
                     /gene="xseA"
                     /locus_tag="ACHKJF_000243"
     CDS             4487..5857
                     /gene="xseA"
                     /locus_tag="ACHKJF_000243"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP0947669.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5854..6069)
                     /gene="yfgJ"
                     /locus_tag="ACHKJF_000244"
     CDS             complement(5854..6069)
                     /gene="yfgJ"
                     /locus_tag="ACHKJF_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0947670.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6138..7610)
                     /gene="der"
                     /locus_tag="ACHKJF_000245"
     CDS             complement(6138..7610)
                     /gene="der"
                     /locus_tag="ACHKJF_000245"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP0947671.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7728..8906)
                     /gene="bamB"
                     /locus_tag="ACHKJF_000246"
     CDS             complement(7728..8906)
                     /gene="bamB"
                     /locus_tag="ACHKJF_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP0947672.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8917..9537)
                     /gene="yfgM"
                     /locus_tag="ACHKJF_000247"
     CDS             complement(8917..9537)
                     /gene="yfgM"
                     /locus_tag="ACHKJF_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP0947673.1"
                     /translation="MEICENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9555..10829)
                     /gene="hisS"
                     /locus_tag="ACHKJF_000248"
     CDS             complement(9555..10829)
                     /gene="hisS"
                     /locus_tag="ACHKJF_000248"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP0947674.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10940..12058)
                     /gene="ispG"
                     /locus_tag="ACHKJF_000249"
                     /gene_synonym="gcpE"
     CDS             complement(10940..12058)
                     /gene="ispG"
                     /locus_tag="ACHKJF_000249"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP0947675.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12085..13098)
                     /gene="rodZ"
                     /locus_tag="ACHKJF_000250"
     CDS             complement(12085..13098)
                     /gene="rodZ"
                     /locus_tag="ACHKJF_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP0947676.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13383..14537)
                     /gene="rlmN"
                     /locus_tag="ACHKJF_000251"
     CDS             complement(13383..14537)
                     /gene="rlmN"
                     /locus_tag="ACHKJF_000251"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP0947677.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14687..15118)
                     /gene="ndk"
                     /locus_tag="ACHKJF_000252"
     CDS             complement(14687..15118)
                     /gene="ndk"
                     /locus_tag="ACHKJF_000252"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP0947678.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15267..17579)
                     /gene="pbpC"
                     /locus_tag="ACHKJF_000253"
     CDS             complement(15267..17579)
                     /gene="pbpC"
                     /locus_tag="ACHKJF_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP0947679.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17580..22541)
                     /gene="yfhM"
                     /locus_tag="ACHKJF_000254"
     CDS             complement(17580..22541)
                     /gene="yfhM"
                     /locus_tag="ACHKJF_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP0947680.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLLENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLYLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22748..23593
                     /gene="sseA"
                     /locus_tag="ACHKJF_000255"
     CDS             22748..23593
                     /gene="sseA"
                     /locus_tag="ACHKJF_000255"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP0947681.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23929..24054
                     /gene="timP"
                     /locus_tag="ACHKJF_000256"
     CDS             23929..24054
                     /gene="timP"
                     /locus_tag="ACHKJF_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP0947682.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24086..24862)
                     /gene="sseB"
                     /locus_tag="ACHKJF_000257"
     CDS             complement(24086..24862)
                     /gene="sseB"
                     /locus_tag="ACHKJF_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP0947683.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25004..26287)
                     /gene="pepB"
                     /locus_tag="ACHKJF_000258"
     CDS             complement(25004..26287)
                     /gene="pepB"
                     /locus_tag="ACHKJF_000258"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP0947684.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26465..26665)
                     /gene="iscX"
                     /locus_tag="ACHKJF_000259"
     CDS             complement(26465..26665)
                     /gene="iscX"
                     /locus_tag="ACHKJF_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP0947685.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26677..27012)
                     /gene="fdx"
                     /locus_tag="ACHKJF_000260"
     CDS             complement(26677..27012)
                     /gene="fdx"
                     /locus_tag="ACHKJF_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP0947686.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27014..28864)
                     /gene="hscA"
                     /locus_tag="ACHKJF_000261"
     CDS             complement(27014..28864)
                     /gene="hscA"
                     /locus_tag="ACHKJF_000261"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP0947687.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28881..29396)
                     /gene="hscB"
                     /locus_tag="ACHKJF_000262"
     CDS             complement(28881..29396)
                     /gene="hscB"
                     /locus_tag="ACHKJF_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP0947688.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29492..29815)
                     /gene="iscA"
                     /locus_tag="ACHKJF_000263"
     CDS             complement(29492..29815)
                     /gene="iscA"
                     /locus_tag="ACHKJF_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP0947689.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29832..30218)
                     /gene="iscU"
                     /locus_tag="ACHKJF_000264"
     CDS             complement(29832..30218)
                     /gene="iscU"
                     /locus_tag="ACHKJF_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP0947690.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30246..31460)
                     /gene="iscS"
                     /locus_tag="ACHKJF_000265"
     CDS             complement(30246..31460)
                     /gene="iscS"
                     /locus_tag="ACHKJF_000265"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP0947691.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31572..32060)
                     /gene="iscR"
                     /locus_tag="ACHKJF_000266"
     CDS             complement(31572..32060)
                     /gene="iscR"
                     /locus_tag="ACHKJF_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP0947692.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32340..33080)
                     /gene="trmJ"
                     /locus_tag="ACHKJF_000267"
     CDS             complement(32340..33080)
                     /gene="trmJ"
                     /locus_tag="ACHKJF_000267"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP0947693.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33199..34002
                     /gene="suhB"
                     /locus_tag="ACHKJF_000268"
     CDS             33199..34002
                     /gene="suhB"
                     /locus_tag="ACHKJF_000268"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP0947694.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34147..35001
                     /gene="yfhR"
                     /locus_tag="ACHKJF_000269"
     CDS             34147..35001
                     /gene="yfhR"
                     /locus_tag="ACHKJF_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0947695.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35192..36472
                     /gene="csiE"
                     /locus_tag="ACHKJF_000270"
     CDS             35192..36472
                     /gene="csiE"
                     /locus_tag="ACHKJF_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP0947696.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36464..37603)
                     /gene="hcaT"
                     /locus_tag="ACHKJF_000271"
     CDS             complement(36464..37603)
                     /gene="hcaT"
                     /locus_tag="ACHKJF_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP0947697.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37763..38653)
                     /gene="hcaR"
                     /locus_tag="ACHKJF_000272"
     CDS             complement(37763..38653)
                     /gene="hcaR"
                     /locus_tag="ACHKJF_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP0947698.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPVYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38789..40150
                     /gene="hcaE"
                     /locus_tag="ACHKJF_000273"
     CDS             38789..40150
                     /gene="hcaE"
                     /locus_tag="ACHKJF_000273"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP0947699.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40147..40665
                     /gene="hcaF"
                     /locus_tag="ACHKJF_000274"
     CDS             40147..40665
                     /gene="hcaF"
                     /locus_tag="ACHKJF_000274"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP0947700.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40665..40985
                     /gene="hcaC"
                     /locus_tag="ACHKJF_000275"
     CDS             40665..40985
                     /gene="hcaC"
                     /locus_tag="ACHKJF_000275"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP0947701.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            40982..41794
                     /gene="hcaB"
                     /locus_tag="ACHKJF_000276"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             40982..41794
                     /gene="hcaB"
                     /locus_tag="ACHKJF_000276"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP0947702.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41804..43006
                     /gene="hcaD"
                     /locus_tag="ACHKJF_000277"
     CDS             41804..43006
                     /gene="hcaD"
                     /locus_tag="ACHKJF_000277"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP0947703.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43103..43525
                     /gene="yphA"
                     /locus_tag="ACHKJF_000278"
     CDS             43103..43525
                     /gene="yphA"
                     /locus_tag="ACHKJF_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0947704.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43573..44445)
                     /gene="yphB"
                     /locus_tag="ACHKJF_000279"
     CDS             complement(43573..44445)
                     /gene="yphB"
                     /locus_tag="ACHKJF_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0947705.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44457..45551)
                     /gene="yphC"
                     /locus_tag="ACHKJF_000280"
     CDS             complement(44457..45551)
                     /gene="yphC"
                     /locus_tag="ACHKJF_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0947706.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDKVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINSPD"
     gene            complement(45584..46582)
                     /gene="yphD"
                     /locus_tag="ACHKJF_000281"
     CDS             complement(45584..46582)
                     /gene="yphD"
                     /locus_tag="ACHKJF_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0947707.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     LGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46607..48118)
                     /gene="yphE"
                     /locus_tag="ACHKJF_000282"
     CDS             complement(46607..48118)
                     /gene="yphE"
                     /locus_tag="ACHKJF_000282"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0947708.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48141..49124)
                     /gene="yphF"
                     /locus_tag="ACHKJF_000283"
     CDS             complement(48141..49124)
                     /gene="yphF"
                     /locus_tag="ACHKJF_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0947709.1"
                     /translation="MPTKIRTTRNLLLMATLLGSALFARAAEKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGGDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAKIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVLAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49221..52502)
                     /gene="yphG"
                     /locus_tag="ACHKJF_000284"
     CDS             complement(49221..52502)
                     /gene="yphG"
                     /locus_tag="ACHKJF_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP0947710.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDYQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52620..53813
                     /gene="yphH"
                     /locus_tag="ACHKJF_000285"
     CDS             52620..53813
                     /gene="yphH"
                     /locus_tag="ACHKJF_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP0947711.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53877..55130)
                     /gene="glyA"
                     /locus_tag="ACHKJF_000286"
     CDS             complement(53877..55130)
                     /gene="glyA"
                     /locus_tag="ACHKJF_000286"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP0947712.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55458..56648
                     /gene="hmpA"
                     /locus_tag="ACHKJF_000287"
     CDS             55458..56648
                     /gene="hmpA"
                     /locus_tag="ACHKJF_000287"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP0947713.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56693..57031)
                     /gene="glnB"
                     /locus_tag="ACHKJF_000288"
     CDS             complement(56693..57031)
                     /gene="glnB"
                     /locus_tag="ACHKJF_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP0947714.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57092..58426)
                     /gene="glrR"
                     /locus_tag="ACHKJF_000289"
     CDS             complement(57092..58426)
                     /gene="glrR"
                     /locus_tag="ACHKJF_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP0947715.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58416..59129)
                     /gene="qseG"
                     /locus_tag="ACHKJF_000290"
     CDS             complement(58416..59129)
                     /gene="qseG"
                     /locus_tag="ACHKJF_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP0947716.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTLAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PREGEKPAPSTHEVTPDEP"
     gene            complement(59293..60720)
                     /gene="qseE"
                     /locus_tag="ACHKJF_000291"
     CDS             complement(59293..60720)
                     /gene="qseE"
                     /locus_tag="ACHKJF_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP0947717.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61296..65183)
                     /gene="purL"
                     /locus_tag="ACHKJF_000292"
                     /gene_synonym="purI"
     CDS             complement(61296..65183)
                     /gene="purL"
                     /locus_tag="ACHKJF_000292"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP0947718.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65441..66997
                     /gene="mltF"
                     /locus_tag="ACHKJF_000293"
     CDS             65441..66997
                     /gene="mltF"
                     /locus_tag="ACHKJF_000293"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP0947719.1"
                     /translation="MKKLKINYLFIGILALLLVVALWPSIPWFGKADNRIAAIQARGQ
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66994..67497)
                     /gene="tadA"
                     /locus_tag="ACHKJF_000294"
     CDS             complement(66994..67497)
                     /gene="tadA"
                     /locus_tag="ACHKJF_000294"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP0947720.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67555..68190)
                     /gene="yfhb"
                     /locus_tag="ACHKJF_000295"
     CDS             complement(67555..68190)
                     /gene="yfhb"
                     /locus_tag="ACHKJF_000295"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP0947721.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68399..69247
                     /gene="yfhH"
                     /locus_tag="ACHKJF_000296"
     CDS             68399..69247
                     /gene="yfhH"
                     /locus_tag="ACHKJF_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0947722.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69303..69563
                     /gene="yfhL"
                     /locus_tag="ACHKJF_000297"
     CDS             69303..69563
                     /gene="yfhL"
                     /locus_tag="ACHKJF_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP0947723.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69757..69837)
                     /gene="shoB"
                     /locus_tag="ACHKJF_000298"
     CDS             complement(69757..69837)
                     /gene="shoB"
                     /locus_tag="ACHKJF_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP0947724.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70257..70637)
                     /gene="acpS"
                     /locus_tag="ACHKJF_000299"
     CDS             complement(70257..70637)
                     /gene="acpS"
                     /locus_tag="ACHKJF_000299"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP0947725.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70637..71368)
                     /gene="pdxJ"
                     /locus_tag="ACHKJF_000300"
     CDS             complement(70637..71368)
                     /gene="pdxJ"
                     /locus_tag="ACHKJF_000300"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP0947726.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71380..72108)
                     /gene="recO"
                     /locus_tag="ACHKJF_000301"
     CDS             complement(71380..72108)
                     /gene="recO"
                     /locus_tag="ACHKJF_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP0947727.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72120..73025)
                     /gene="era"
                     /locus_tag="ACHKJF_000302"
     CDS             complement(72120..73025)
                     /gene="era"
                     /locus_tag="ACHKJF_000302"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP0947728.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73022..73702)
                     /gene="rnc"
                     /locus_tag="ACHKJF_000303"
     CDS             complement(73022..73702)
                     /gene="rnc"
                     /locus_tag="ACHKJF_000303"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP0947729.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73975..74949)
                     /gene="lepB"
                     /locus_tag="ACHKJF_000304"
     CDS             complement(73975..74949)
                     /gene="lepB"
                     /locus_tag="ACHKJF_000304"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP0947730.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(74965..76764)
                     /gene="lepA"
                     /locus_tag="ACHKJF_000305"
     CDS             complement(74965..76764)
                     /gene="lepA"
                     /locus_tag="ACHKJF_000305"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP0947731.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76962..77441)
                     /gene="rseC"
                     /locus_tag="ACHKJF_000306"
     CDS             complement(76962..77441)
                     /gene="rseC"
                     /locus_tag="ACHKJF_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP0947732.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77438..78394)
                     /gene="rseB"
                     /locus_tag="ACHKJF_000307"
     CDS             complement(77438..78394)
                     /gene="rseB"
                     /locus_tag="ACHKJF_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP0947733.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKVNLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78394..79044)
                     /gene="rseA"
                     /locus_tag="ACHKJF_000308"
     CDS             complement(78394..79044)
                     /gene="rseA"
                     /locus_tag="ACHKJF_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP0947734.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79077..79652)
                     /gene="rpoE"
                     /locus_tag="ACHKJF_000309"
     CDS             complement(79077..79652)
                     /gene="rpoE"
                     /locus_tag="ACHKJF_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP0947735.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79649..79804)
                     /gene="rseD"
                     /locus_tag="ACHKJF_000310"
     CDS             complement(79649..79804)
                     /gene="rseD"
                     /locus_tag="ACHKJF_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP0947736.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80060..81682
                     /gene="nadB"
                     /locus_tag="ACHKJF_000311"
     CDS             80060..81682
                     /gene="nadB"
                     /locus_tag="ACHKJF_000311"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP0947737.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81667..82404)
                     /gene="trmN"
                     /locus_tag="ACHKJF_000312"
     CDS             complement(81667..82404)
                     /gene="trmN"
                     /locus_tag="ACHKJF_000312"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP0947738.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSGSVIIDAVELESEAAAQAQENINQSPWVERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82536..83870
                     /gene="srmB"
                     /locus_tag="ACHKJF_000313"
     CDS             82536..83870
                     /gene="srmB"
                     /locus_tag="ACHKJF_000313"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP0947739.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84079..84960)
                     /gene="yfiE"
                     /locus_tag="ACHKJF_000314"
     CDS             complement(84079..84960)
                     /gene="yfiE"
                     /locus_tag="ACHKJF_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0947740.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRSTVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEESFVAG"
     gene            85063..85650
                     /gene="eamB"
                     /locus_tag="ACHKJF_000315"
     CDS             85063..85650
                     /gene="eamB"
                     /locus_tag="ACHKJF_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP0947741.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            complement(85706..86089)
                     /gene="grcA"
                     /locus_tag="ACHKJF_000316"
     CDS             complement(85706..86089)
                     /gene="grcA"
                     /locus_tag="ACHKJF_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP0947742.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86394..87083
                     /gene="ung"
                     /locus_tag="ACHKJF_000317"
     CDS             86394..87083
                     /gene="ung"
                     /locus_tag="ACHKJF_000317"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP0947743.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87131..88168)
                     /gene="yfiF"
                     /locus_tag="ACHKJF_000318"
     CDS             complement(87131..88168)
                     /gene="yfiF"
                     /locus_tag="ACHKJF_000318"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0947744.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88375..88794
                     /gene="trxC"
                     /locus_tag="ACHKJF_000319"
     CDS             88375..88794
                     /gene="trxC"
                     /locus_tag="ACHKJF_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP0947745.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            88863..89561
                     /gene="tapT"
                     /locus_tag="ACHKJF_000320"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88863..89561
                     /gene="tapT"
                     /locus_tag="ACHKJF_000320"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP0947746.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89593..92253
                     /gene="pat"
                     /locus_tag="ACHKJF_000321"
     CDS             89593..92253
                     /gene="pat"
                     /locus_tag="ACHKJF_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP0947747.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92367..93722
                     /gene="pssA"
                     /locus_tag="ACHKJF_000322"
     CDS             92367..93722
                     /gene="pssA"
                     /locus_tag="ACHKJF_000322"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP0947748.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93768..94091
                     /gene="yfiM"
                     /locus_tag="ACHKJF_000323"
     CDS             93768..94091
                     /gene="yfiM"
                     /locus_tag="ACHKJF_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP0947749.1"
                     /translation="MRIPFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTIW
                     QLTRH"
     gene            complement(94088..95386)
                     /gene="kgtP"
                     /locus_tag="ACHKJF_000324"
     CDS             complement(94088..95386)
                     /gene="kgtP"
                     /locus_tag="ACHKJF_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP0947750.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        22825 a        26080 c        24043 g        22714 t
ORIGIN      
        1 gatcttaaat gtcttatttt tcgtaatgtg tataacaagg aatagtgatg aaatttaaaa
       61 aatgtcttct gcctgtggca atgttagcgt cattcactct ggcaggatgc cagtcaaatg
      121 ctgacgatca tgccgccgat gtttatcaaa ccgatcaact gaataccaaa caagaaacta
      181 aaaccgttaa tattatttcc attcttcccg caaaagttgc cgtagacaac tcccaaaata
      241 aacggaacgc acaagccttc ggcgcgctta ttggcgcagt cgctggcggt gttatcggcc
      301 acaacgtcgg ttctggcagc aattccggaa cgacggcagg ggcagttggc ggcggagctg
      361 taggcgcggc agtgggttct atggtgaatg ataaaacctt agtggaaggt gtttctttaa
      421 catataagga aggcaccaaa gtgtatacct ctacccaggt gggtaaagag tgccagttta
      481 cgacaggttt agccgttgtt attaccacaa cgtataacga aacgcgtatt cagccaaata
      541 ctaaatgtcc tgaaaagagc taataatcag gaggagtcat gaagaaagtt tttctttgcg
      601 ccatcttggc ctccttaagc tatccggcta tcgcctcatc attgcaggat caactctctg
      661 ctgtagcaga agctgaacag caaggtaaaa atgaagagca aaggcagcat gacgaatggg
      721 tcgcggagcg caacagggaa atccagcaag agaagcaacg tcgcgcaaat gcccaggccg
      781 ccgctaacaa aagagcggca acggcagcgg caaataagaa agctcgtcag gataaactgg
      841 acgccgaagc ctctgcggac aaaaaacgcg atcaaagtta tgaagatgag ctacgcagct
      901 tagagattca gaaacaaaaa ctggcgctgg caaaagaaga agcccgcgtt aagcgagaaa
      961 acgaatttat cgatcaggaa ctgaagcaca aagctgcgca aaccgatgtg gtgcaatctg
     1021 aagctgacgc caacagaaat atgactgaag gcggtcgcga tctgatgaaa agcgtgggca
     1081 aagcagaaga gaacaaatcg gacagctggt ttaattaatc gatgttagta acttcaagcc
     1141 tatgattctt gaagataaaa aaccctctgt agtaacagag ggttttgttc attcatagtg
     1201 cagggattaa aatcattccc actcaatggt agctggcggc ttgccgctga tgtcatacac
     1261 cacgcgggaa ataccgttca cttcattgat aatgcggttg gaaacgcgac cgaggaaatc
     1321 gtacggcaga tgcgcccagt gtgcggtcat aaagtcgatg gtttcgacag cacgcagaga
     1381 gacaacccag tcatacttac gaccatcgcc cattacgcca acggaacgta ctggcaggaa
     1441 gacggtgaac gcctggctga ctttgtcgta caggtccgct ttacgcagtt cttcaatgaa
     1501 gatggcgtca gcacggcgca gcaggtcaca gtactctttc ttcacttcac ccagaacacg
     1561 aacgccaagg cctggtcccg ggaacgggtg acggtacagc atgtcgtacg gtagacccag
     1621 ctccagacca atcttacgca cttcgtcttt gaacagctct ttcagcggtt caaccaggcc
     1681 catcttcatc tctttcggca ggccgcccac gttgtggtga gatttgatga cgtgtgcttt
     1741 accggttgca gacgccgcag attcgataac gtcagggtag atggtgccct gtgccagcca
     1801 cttcacgtct tccagtttca gcgcttcttc atcgaatact tcaacgaaaa cgcgcccgat
     1861 gattttacgt tttgcttccg gatcgttttc gccagccagc gctgacagga agcgatcttc
     1921 tgccggtacg tgaacaatgt taagaccaaa gtgatcgcca aacatatcca gcacctgctc
     1981 tgcttcgttg agacgcagca ggccgttgtc gacgaatacg caagtcaggt ttttaccgat
     2041 agcgcggtgc agcagcattg cggttacgga ggaatccaca ccaccagaga ggccgaggat
     2101 gactttatcg tcgcctacct gctcgcggat gcgagctaca gcatcgtcga taattttcgc
     2161 tggcgtccac agggcttcac actggcagat atcacgcaca aaacgctcca gcatgcgcat
     2221 accctggcgg gtgtgagtca cttccgggtg gaactgtacg ccatagaagc gtttttcttc
     2281 gttagccata atggcaaacg ggcagctttc ggtgctggct acggtgacga agtcggacgg
     2341 gatagcggta actttatcgc cgtggctcat ccagacatcg agcagcggtt taccgtctgc
     2401 ggtcagcgca tcttcgatac cgcgaaccag tgcgctgtcg tttacgactt caacctgcgc
     2461 gtagccaaat tcacgttcgt tagaggcttc aacgtgaccg cccaactgca ttgccatggt
     2521 ctgcatgcca tagcaaacgc cgaataccgg tacgcctgct tcaaagacat actgcggcgc
     2581 acgcgggctg ttttcttcgg tggtgctttc cgggccgccg gaaagaataa tgccgcttgg
     2641 attgaagtca cgaatttgtg cttctgtcac atcccacgcc cacagttcgc agtaaacacc
     2701 cagctcacgc acgcggcgcg caaccagttg agtgtactga gaaccgaagt ccagaatgag
     2761 gatgcgatgc ttatgaatgt tttccgtcat tgacgcttat tccgaggcaa gtgaaacaga
     2821 taatataaat cgcccgacat gaagtcgggc gaagagaatc aggagcccag acggtagttc
     2881 ggggactctt tagtaatggt cacgtcgtga acgtggcttt cctgaatgcc cgcaccgctg
     2941 atacgtacaa actccgcttt agtacgcagt tcgtcgatag taccacagcc ggtcagaccc
     3001 atacaggagc gcaggccgcc catctgctgg tgaatgatct ctttcaggcg acctttataa
     3061 gctacgcgac cttcgatacc ttccggcacc agtttgtcgg cagcgttatc gctctggaaa
     3121 taacggtcag aggaaccttt ggacatcgcg cccagggaac ccataccacg gtaagatttg
     3181 taagaacggc cctggtagag ttcgatttca cccggagatt cttcagtacc cgccagcatg
     3241 gaacctacca tcaccgcgct tgcgccagcg gcgatagctt tggcgatgtc gccggagaag
     3301 cgaataccac catctgcgat aaccggaata ccggtgcctt ccagggcttc aactgcgtca
     3361 gcaacggcag tgatctgcgg aacacctacg ccagtaacga tacgagtcgt acagatagaa
     3421 ccagggccga taccgacttt aaccgcgctg caaccagctt ctgccaaagc gcgtgcacct
     3481 gcagctgttg ccacgttgcc gccgatgatt tgcagatccg gatatttagc acgggtttca
     3541 cggatacgct gcaaaacgcc ttctgagtga ccgtgggagg agtcgatcag cagaacgtca
     3601 acgccagcgg caaccagcgc gtcaacacgc tcttcgttac ccgcacctgc gccaactgca
     3661 gcaccaacac gcagacggcc ttgctcgtct ttacaggcgt tcggtttacg ttccgctttc
     3721 tggaagtctt tcacggtgat catgccgatc aggtggaatt cgtcatcaac caccagcgct
     3781 ttttcaacgc gtttttcgtg catttttgcc agcaccactt cacgggcttc accttcacgc
     3841 acggtgacca gacgctcttt cggcgtcatg taaacgctaa ccggctggtt caggtcggta
     3901 acaaaacgca cgtcacgacc ggtgataata cccaccagtt cgttttcttc ggtaacgacc
     3961 ggatagcccg caaaaccgtt acgctcagtc agttctttca cttcgcgcag cgtcgtggtt
     4021 ggcagaacag tctgcggatc agtcaccaca ccagattcgt gttttttcac acggcgaact
     4081 tcttctgcct ggcgttcaat ggacatgttt ttgtggataa agccgatacc gccttcctga
     4141 gccagagcaa tagccaggcg cgcttccgtt acggtatcca ttgctgcgga aagcataggg
     4201 atattcagac gaatagtttt cgtcagctgg gtgctgaggt cagcagtatt cggcagaacg
     4261 gtagaatgag caggaacgag gagaacgtcg tcaaacgtca gagcttcttt agcgatacgt
     4321 agcatgggca atatctcgac cagagtggtt aataaatatt gccgcggcat tatacagagc
     4381 gtaaccgatt gcatctaccc ctttttgcaa aaaatgcttg ctatccccga agggcgggtt
     4441 actatcgact gaataacctg ctgatttaga atttgatctc gctcacatgt taccttctca
     4501 atcccctgca atttttaccg ttagtcgcct gaatcaaacg gttcgtctgc tgcttgagca
     4561 tgagatggga caggtttgga tcagcggcga aatctctaat ttcacacaac cggcttccgg
     4621 tcactggtac tttacactca aagacgacac cgcccaggta cgctgcgcga tgttccgcaa
     4681 cagcaaccgc cgggtgacct tccgtccaca gcatggacaa caagttttag ttcgcgccaa
     4741 tattacgctc tacgagccgc gcggtgacta ccagataatc gttgagagta tgcagccggc
     4801 cggtgaaggg ctgctgcaac agaagtacga acagctcaaa gcgaagttgc aggctgaagg
     4861 tttgttcgat cagcaataca aaaaaccact tccctcccct gcgcattgcg ttggtgtgat
     4921 cacctcaaaa accggtgctg cgctacatga tattttgcat gtgttaaaac gtcgcgatcc
     4981 ttctctaccg gtgatcatct accccaccgc cgttcagggc gatgacgcac cggggcaaat
     5041 tgttcgcgcc attgaactgg cgaatcagcg caatgagtgc gacgtgttga tcgttgggcg
     5101 cggcggcggt tcgctggaag atttatggag ttttaacgac gaacgcgtag cgcgggcgat
     5161 ttttgccagc cgcattccgg tcgtcagcgc cgtcgggcat gagacggatg tgaccattgc
     5221 cgattttgtt gccgatctgc gtgcgccaac gccgtctgcc gccgctgaag tagtgagccg
     5281 taatcagcaa gagttactgc gccaggtgca atcgacccgt caacggctgg agatggcgat
     5341 ggattattat ctcgccaacc gcacgcgtcg ctttacgcag atccatcacc gattacagca
     5401 acagcatccg cagctccggc tggcacgcca gcaaaccatg cttgagcgcc tgcaaaagcg
     5461 gatgagcttt gcgctggaaa atcaacttaa gcgtaccggg caacagcagc agcggttaac
     5521 acagcggctg aatcagcaaa atccacagcc gaagattcat cgcgcgcaaa cgcgcattca
     5581 gcaactggaa tatcgtttag cagaaaccct gcgcgtacag cttagcgcca cgcgtgaacg
     5641 tttcggtaat gcagtaacgc acctcgaagc cgtaagccca ctgtcaacgc tggcgcgtgg
     5701 atacagcgtt actactgcta ctgacggcaa tgtactgaaa aaagtgaagc aagttaaagc
     5761 gggtgaaatg ctaaccacac gtctggaaga cggctggata gaaagtgaag taaaaaacat
     5821 ccagccggta aaaaaatcgc gtaaaaaggt gcattaagcc agcacgaatt caacgcgttt
     5881 tttcgatatc aagccatgcc cgtgctgaca gaagtaatct actgcaccgc aggcttttaa
     5941 cacctgcaac ggttgatggc agtctggaca aagagctttc atttcaataa tttccccgca
     6001 ggatgggcaa cgcgcatggt cgttatcctg atcaagcaca tgctgacatt gtggacaatg
     6061 cagttccata tgaattcctc cggtagccat ccctctacat tcatagaggg aatggcagat
     6121 aaaatactta cggataatta tttatttttc ttgatgtgct tcatcagacg cttacgttta
     6181 cgcatctggg ttggcgtcag ggtgttacgc ttgttcgcat acgggttttc cccttctttg
     6241 aactgaatac gaatcggcga tcccattacg tccagcgatt tgcggaagta gttcatcaag
     6301 tagcgcttgt aggaatcagg caggtctttc acctgattac cgtgaatcac cacaatcggc
     6361 gggttataac caccagcgtg ggcatatttc agcttcacac gacgaccgcg taccagcggc
     6421 ggttggtgat cttcaacagc catcgtcatg atgcgcgtca gcatagaggt ccccacacga
     6481 cgggtggagc tgtcatacgc ttcacgtact gattcaaaca agttaccaac accactgccg
     6541 tgcaaggcag agataaagtg cacacgagca aaatcgataa agcccagacg gaagtccagc
     6601 gtttctttca cctgctcttt cacttcctgg ctcaggccat cccacttatt caccacaatg
     6661 acaagtgagc gcccactatt gagaataaag cccagcagcg agagatcctg atcggaaata
     6721 ccttcgcgcg catcaatcac caacatcacc acgttggcat cttcaatggc ctgcaacgtt
     6781 ttgattacgg agaatttctc tacagcatcg gtgattttgc cgcgtttacg tacgccagcg
     6841 gtgtcaatga gcacatactc acgtccatcg cgttccattg ggatatagat gctgtcacgc
     6901 gtcgtaccag gcatgtcata aacaacaacg cgctcttcac caagaatacg gttagtgagt
     6961 gtagacttac ctacgttcgg acgacccaca atcgccagtt tgatcggcag actttgcggg
     7021 tcaaagtcgt cttcctcttc ttcttcgccg ttctcttccg cttcaaattg cgcccagtat
     7081 tcagcgtctt cgtcgacttc ctcttgcggt gcgagatctt ccatccacgg cagcagcaca
     7141 tgctccagca gacttaatac gccacgaccg tgagacgcgg cgatcgggta gatttcacct
     7201 aagccaagcg agtagaaatc aaccactgcc tgatcgggat ccagaccgtc agttttgttt
     7261 gccaccagga aggtcggttt ttcacgggag cgcagatgtt tggcaatcgc ttcatctgcc
     7321 ggcatcaggc ccgcgcgcgc atccaccata aacagtacga cgtccgcttc ttcaatcgcc
     7381 agcagcgact gttccgccat gcgggtttct acaccgtctt ctgtgccatc aatcccgccg
     7441 gtatcaatac agataaactc acggccttca atttccgcac gaccgtactt acggtcacga
     7501 gtcagacccg ggaaatccgc aaccagcgca tctcgggtgc gagttagacg gttaaataac
     7561 gtggattttc ctacgttagg gcgcccgaca agcgcgacca caggtaccat gtttaaagcc
     7621 tcatttttat aaatcatcag acaacgcacg ctatattcgc gtcgttgtta aaaacaggaa
     7681 aacggcccct gtccaggagc cgttttcaaa gtgaacgaca gagacgatta acgtgtaata
     7741 gagtacacgg ttccgtcttt tgcctggatc agcagtttgc cgtcagcggc aaccggttca
     7801 gtctggaaac cggaactatc aactttttgc tgggcaacga aacgaccatc ttcgacgtta
     7861 atccagtgca gataaccttc actgtcaccg accaccaggt tgccattata cagcaccgga
     7921 gaagtcagca ggcgatgcag cagatcgctt tgtgtccaca gcgtaacgcc gccatcaatg
     7981 gtcaacgcca tcaccctgtc attttgatcg accagataga tgcgattgcc gtcgacgatg
     8041 aaatcattca ccgaacccag ttcgcgtttc cacataatct gaccactgcg cagatcaagc
     8101 gccgtcaggt taccattata ggccagcgcg aaaacaacgc cgttaacaac gacgggagtc
     8161 gtgtcaacat cgctcagacg gtcaatttcg gtagaaccgg tcgcctggga aatacgctgc
     8221 tgccaaatca tctggccctg ttccatcagc actgcgctga cgcgaccatt atcgccaccc
     8281 acgacggccg caccaaaagc cgttgccggc gcagactcgc cacgcaaaga gagcgaaggc
     8341 atatcgaggt taactgtcca tttgacagcg ccgtcagcct cgttcagcgc ttgtaactga
     8401 ccgttactgg tgtggattaa caccagaccg tcgctgacca ccgggcgcga aagtgcttca
     8461 cccgcgactt tagtttgcca tgccacagta ccatcgctgg tattcagcgc gtaaacctgc
     8521 gccttttcgc tgccaatgta gacatgccca ccagacacgg tcagaccgcc agaaagtaat
     8581 gcaggctctt tagagaacca gccatctttc tcggccaggc tgacagacca gatttctttg
     8641 ccatcatccg cattcagcgc ttttaccaaa ccagcgcggt ccgctgcata gacaacgttg
     8701 tccgccagtg ccggatgaag attggaatag aagttgccaa tgccgctacc aacggaagtg
     8761 ctccacgccg tggtcggcgt aaactggttt tcaacggttg gcaatgggga catctttacc
     8821 acatcttctt cgctgttaaa cagcgaacag ccgcttaaaa gggtaacgga aagcagtcct
     8881 ggcagcagta atttacgcaa ttgcatcggg tccctctcag atggacaaat tattaatttt
     8941 catctgcatc atttcgctca gtgccggagt aacatcgctt ttcacgcctg cttcccatgc
     9001 actacgcgca ccttgcttat cacctttgct cagcaatgct tcaccacgca ggtcggcaac
     9061 aatggcagcc cacccttcac ctttgatggt atcaagggtt ttcagcgcgg catcagcctg
     9121 cttgagctgt acctgaacgc gagcaagacg cagatttatc acggctttga gattttcatc
     9181 gctcgtgtct gccagcccct gttgtaactg ggcggcagct ttctctagtt catttttgtc
     9241 aacaaattgc tgcgcaagtt ccaaagaagc cagcgcacca taagtatttt tattttcagc
     9301 agcaaatttt tccgccgccg ggatgctatc cggtttgcct tcgctcactg cggtaaccgc
     9361 attttgatag gcaagagaag cggagcgtgc agaatcaacc tgatggctgt tccagtagcg
     9421 ccagccaatc agtgcgccaa cgcccaaaat caccccaaca gccagtgctt tgccattttc
     9481 agcaaaaaag cgtttaaccg cttctacctg gtcgttttcg ttctcgcaaa tttccacgct
     9541 gtccttctcc ttccttaacc cagtaacgtg cgcaaatgcg cggctacgct atcctgcgca
     9601 actgccgttt gctcaccaga gcgcaaatcc ttcactactg ctgtgccgtt agccacttca
     9661 gactcaccca gcaccacagc aacgcgggca ccccatttat cagcacgggc aaactgtttc
     9721 ttaaagttgc cgccgccgtg gttggtcatc aatttcacgc ccggtaattc atcacgcaga
     9781 cgctcagcta atgccatagc cgcagattgt gtatcagcac ctgaagccac caggtatata
     9841 tcgacaacag gatcggcttt aaattccgga ttaacggcct gtactaacaa tacaagacgt
     9901 tcgaggccca tcgcaaaacc gacagccggt gttgcacgac cgcccagttg ttccacaaga
     9961 ccgtcataac gaccgcctgc acacacggtg ccctgggagc cgagactgtt agtcacccac
    10021 tcgaaaacgg tacggttata gtaatccaga ccacgcacca gacgctggtt tacggtgtaa
    10081 gcgatccccg cgctctcaag cagtttgcac agaccggcaa aatgctcacg agattcctcg
    10141 tccagataat cacctaatgc cggagcgtcg ttgagaagcg cctgcacttc cggatttttt
    10201 gaatccagca cgcgcagcgg gttagtgtac atgcggcgtt tgcagtcttc gtccagcttt
    10261 tctttatgct gctcaaggaa tgccaccagc gcatcgcggt aattggcgcg tgcttccagc
    10321 gaaccgatag aattcagctc aagagttacg tgttcggaaa tacccagcgc gcgccaccag
    10381 cgggcggtga gcataatcag ttcagcgtcg atatccggac cttgcagacc gaaaacttcg
    10441 cagcccaact gatggaactg acgataacgc cctttctgcg gacgctcgtg acggaacatc
    10501 ggcccgatat accacagacg ctgttcctga ttgtacagaa gaccatgctc gatgccggcg
    10561 cgtacacagc ccgccgtccc ttcagggcgc agagtcaggc tgtcgccatt gcgatcctca
    10621 aaggtgtaca tctctttttc aaccacgtcg gtgacttcac caatcgcacg tttgaatagc
    10681 ggggtctgct ctacaatcgg caagcggatt tcactgtaac cgtagctgcc gagcacgttt
    10741 ttcagtgtgc cttcaatgcg ctgccagatg gccgtttcgc caggcaggta atcgttcatg
    10801 ccgcgaatgg cttgaatgtt ttttgccacg tttattctct ttctgaatat aaaaatgaac
    10861 cctcaacgct tccctcaatg tttcgggagc tatgcgggtt caatcataca cgggaagcga
    10921 ggcgcttccc atcacgttat tatttttcaa cctgctgaac gtcaattcga cgcgcttcgt
    10981 ccagctgact ggctttcgca cgaatgcgtg cttccagctg gtcgatcata tcgttgttgt
    11041 ccagacggtc tttgcgcacg ccatcttcat agaggccgct tttcttgttg ccgccggtga
    11101 cgccgagtgt agaaaccagc gcctcacctg ggccattcac cacgcagccg ataatcgaaa
    11161 cgtccatcgg agtgatgata tcttccaggc gttgctccag cgcgttaacc gtaccgataa
    11221 catcaaattc ctgacgcgaa caggtcgggc aggcgatgaa gttgatacct cgcgaacgga
    11281 tacgcagcga tttcaaaata tcgaaaccga ctttgatctc ttcgaccgga tcggccgcca
    11341 gtgatacgcg cagcgtgtcg ccgatgcctt cagacagcag cagacctaaa ccaatggcgg
    11401 attttactgc cccgctgcgt gcaccaccgg cttcggtgat ccccagatgc agcggctgat
    11461 cgatctgttt tgccagcaaa cgataagact caacagcgag gaagacgtca gacgctttca
    11521 cgctgacttt gaactgatcg aagttcaggc gatcgagatg atcaacatgg cgcatggcgg
    11581 attccagcaa cgcctgcggc gtcggttcgc catacttttc ttgcagatct ttttccagcg
    11641 atccggcgtt aacgccaata cggatcggaa tgtttttatc gcgcgcacag tcaaccacca
    11701 tgcgaatacg ctcttcatta ccgatattgc cagggttaat acgcagacaa tcgacgccgt
    11761 attccgctac tttcagtgca atgcgatagt cgaagtggat gtcagccacc agcggcacgt
    11821 taacctgctg tttgatgagt ttgaacgctt ctgccgcgtc catcgtcggt acggagacac
    11881 ggacgatatc agcgccaacg cgttccagcg ccttgatttg attgaccgtt gcttcgacgt
    11941 ctgtcgtacg cgtattggtc atggactgta cggcgatggg agcaccatcg ccaatcggca
    12001 cattcccaac gtaaatacgt gttgattttc tacgttgaat tggagcctgg ttatgcatga
    12061 aaaatctccc gcgttacccg tctgttactg cgccggtgat tgttcggcat tgagggtcag
    12121 acgcgcaacc tggttagttc tgataaaacg actcagatcg acaggtttcc cttgatactg
    12181 gatctgtact gcggctggcg caccaatttt cagtttgtat ggtgcctggc cggttaagtt
    12241 caaattaccg tctttacgct gcataccgct aaacaatttt ttaccggtag catcagtaac
    12301 ctccagccag caatcggcag taaagttcat caccagcgca ttcggatcag ccgccggcgt
    12361 agtcacgcct gcctgatcgg ttggcaacgg cgcagcgcca tctggcgttg ttgttgccgt
    12421 tggtgccggg gtcgcggcgg tatcaacatt tgcttgcgaa ggcgaaacaa ccgcattctg
    12481 ttgcggatca acagctggtg ctggcgcagt tacggcaggt gtttgcgtgt tggttgcggt
    12541 agtatccaca gacgctggcg gcgtggacgt tgtagccggg tctgtagttg tcgacgtatt
    12601 taacggaaca ctctgcccct gctcgctatt actgctcagt tccgccgaag attgatcggc
    12661 catagtggtg atctcttcct gctgagcttt gtggtcttgc caccaccagg caccgctcag
    12721 gccgataacc acaaacaaca ccagccaggt gaaggtcatc agccagccgt cacgtttttt
    12781 gcggcgttta ccgagggaaa aactctgcat aggcgcaact tttgcagccc gtagtggagc
    12841 ctgcttttcc agccctggca gcagttcttc ttctggaata tgtaccagac gcgcataaga
    12901 gcggatatat ccgcgcagga atgttgaagc aagatcggcg ggtgccttat cttcttcaat
    12961 gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca acggcctgct gactaagtcc
    13021 tagttgttcg cgagcattac gcaggcgagc gccggtagta agtgcttcat tttggtcgtg
    13081 cgtggcttca gtattcattc gctgcaggta cgtttaaatg agaattgaga tgccggtgaa
    13141 ccatcatgct cacccacacc gcgaaacatc cgttaagtta accgttatca tacagtataa
    13201 gaccgtctgt gtgctcgtga caaagcccac attcactaag gctaacttac tgttgcatcg
    13261 ttacatactg ccttaaagtc agcaaaaacg caccgttaat attgaccaga caattgcaac
    13321 ttaatacttc attcataaaa gattacgcca cggtacataa agtaaccgtg gcgtaatggc
    13381 tatcagaccg ctttaatgtc gatggcttca ccctgcatcc gtttacgcag ggtacgtttc
    13441 gtacggtcga taacatcgcc cgccaactga ccacaggcag catcgatatc atcaccacgg
    13501 gttttacgca caatagtggt gaaaccgtag ctcatcagca cttttgagaa acggtcgata
    13561 cggctgttcg agctgcgtcc atacggcgcg cccgggaagg ggttccacgg gatcaggttg
    13621 atcttacacg gcgtatcttt cagcagttcc gccagttggt gcgcgtgttc agtgccgtcg
    13681 ttaacgtggt caagcatcac atactcaata gtgacacggc cctgattggc gttggatttc
    13741 tccagataac ggcgcaccgc agcaaggaac gtttcgatat tgtacttttt gttgatcggc
    13801 acaatttcgt cacgaatttc gtcgttcggc gcgtgcaggg aaatggccag tgcaacgtcg
    13861 atcatatcgc ccagtttatc cagcgccgga actacaccgg aagtagaaag cgtgacgcga
    13921 cgtttagaca ggccaaaacc gaaatcatca agcatgattt ccatcgccgg aacgacgttg
    13981 ttcaggttga gcagcggctc gcccatgccc atcatcacta cgttagtgat tgggcgctga
    14041 ccggtgactt ttgctgcgcc gacgattttc gccgcacgcc acacctggcc aataatttcc
    14101 gatacccgca ggttgcggtt aaagccctgc tgggcggtgg aacagaattt acactccagc
    14161 gcacacccca cctgcgaaga gacgcagagc gtggcacggt cgtcttccgg gatatacacc
    14221 gtttcgacgc gctgatcgcc aacggcgatc gcccatttaa tggtgccgtc ggatgaacgc
    14281 tgttcttcaa ccacttccgg tgcgcggatc tccgccacct ctttcagttt gccgcgcaac
    14341 actttgttga tgtcggtcat ctcatcaaag ttgtcgcagc aatagtgata catccacttc
    14401 atcacctgat cggcgcggaa gggtttttca cctaaatctt taaaaaactc ccgcatctgc
    14461 tgacggttga gatccagcag gttgattttt ccatctttcg tggtgacgtt ttcaggtgtg
    14521 actaattgtt cagacatatg ctattccggc ctcgttatta cacgttatgg cccctggagg
    14581 gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg ggggcgctgc attgtacaaa
    14641 ttctggcgca cggatgccac gtttgcacgc ggcatttacg aaattattaa cgggtgcgcg
    14701 ggcacacttc gccttcgcca aagaaataag cgatttcgcg agcggcagat tcgacggaat
    14761 cagaaccgtg ggtaccgttt tcggtcaggc tgtcagcgta atcagcgcgc agagtaccag
    14821 ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg gtgacgctga acggcgtttt
    14881 caccttccag cacggaaacc acgatcgggc cagaggtcat gaattcaacc agaccatcaa
    14941 agaacggttt tccatcgtgt tcagcataaa agccacgtgc ctgttcaacg gtcaggtgca
    15001 gcattttggt gccgacaatt ttgaaccctg cagcttcaaa gcgcgcaaag atattaccaa
    15061 tgacgttttt tgctaccgcg ttcggtttga tgatggaaaa agtacgttca atagccattt
    15121 ttacctctgt aaattgttct gttgttgtct gtaccagcgt acgagatggc gcggattata
    15181 atgagcaaca gggccgttga ctattgatga aggtaacatt tttttaaaat aaaatgagtt
    15241 ttagcaacaa tcaggggcaa aacagactat tgcatgacaa atttcactgt cgcgatttgc
    15301 cccacatcat ccatcaccag caattgataa tcacctttat ccgtcaaatg cagggtcacg
    15361 ttgcgcccgc gttcagttaa cggttcgcca ttcagaaacc accagcgttc acctgcccct
    15421 ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg gtaaacgttt aataatcgcg
    15481 ccatcgcgaa cgcctgtcag ctgcagaggg agcttggcat cgtgaccgta tggcgggcaa
    15541 attgtcgagg ccggtggtaa gcgcacagcg cgacgttctg atgcgggcag ccagggttcc
    15601 agcggtagcg gccagacatt tatcatttct tgtcgcgctt gcgggcaatc agcggcaaca
    15661 cgtttgccat tttcatccag ccagatgggg aaacgaatgc cattaatgcc ttcctgctcc
    15721 ggcagtaata gagttggcgg ctgactcccg tccagcagcc aggttgccag gcggcggcga
    15781 cagttaccgt caccttccgg caaagactgt ccgcccggcc agcagataac gccacgagtg
    15841 actgagttcg ggcgcgggtc ttccggcaga ttcgcactgc gcgacagtaa gatattattg
    15901 acctgattca acaatggtac ggcactggca aagccaaact gaccaacaac gggcgtgccg
    15961 tccggtctgc cagtccagat cccaatgaca tagcgagcgt taaccccaat cgcccaggcg
    16021 tcacgatagc catagctggt gcccgttttc catgccagtg gggcgacgcg cggcaaggca
    16081 ctatccggca agggtttcgc ttcatcagcc ataatccggc gaatgatcca cgccgccccc
    16141 gacgacatta aaggccgttc aagcagcgga tcgtcaggct gtaagcgcaa tttgcctgcc
    16201 ttgccgtggc gagcaaacgc ggtatacgct gccgccatat cttccagttt tgcaccagcg
    16261 ccgccgagaa tgagtgaaag attcggcgca gcaccgttgg gcaaatataa cggcaatcca
    16321 acattgcgta actttgccgc aaaccgtttc ggtccatagg cttccagcac ctgcacagca
    16381 ggtaagttca gcgagcgcac cagcgcctcg ctcatgctga tcgggccatg aaaaccgcta
    16441 tcaaagttac ctggtcgata atcaccggtg cgccggggga cgtcttgcag cagtgatgcc
    16501 gggtggatca agccttcatc cagcgccaga ccataaacaa acggtttgag caccgatcct
    16561 ggcgatcgga tcgcattgac catatcaaca tgaccaaagc gtgaatcatc gttgagatca
    16621 accgatccca cccagccgcg aacacgcata tcggtatgat caaccacgat catcgccagt
    16681 gagctgcgcg gtggcaaccg ccctttccag ttttgcgcca gttcttccag acgtcgttga
    16741 agaccggcat ccagcgtagt aacgattttg tcgcttttgc ttttaccgag catcatgcgc
    16801 gaaaacagcg gtgccagttg cggcatttgt cggggtgcca gccagatggg ttcttccctt
    16861 gactctttta cctgctcacg ggaccacaca ccttgcacgg ccatccgttc gagcacttta
    16921 ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc gaagacggct gggcgcttgc
    16981 ggcaaaaccg ccagcattgc cgcctcggaa tagcttaaat tcgcaggcga ctttccgaga
    17041 taagcccaac ttgccgcacc gatcccctgc aacgtaccgc caaacggagc gcggttaaga
    17101 tacaaggtca gaatttcacg cttagacaga tgccattcca gttgcaacgc gcgccagagc
    17161 tggcgaattt tgccgccaaa tgttttgggg tgaggatcaa gcagacgagc aacctgcata
    17221 gtgagcgtgc tgccaccgga aataacccgt cccgaagtga gatcttgcca tgctgcgcgc
    17281 gccaccgaga atggattcac ccccggatgc ttccagaacc agcgatcttc ataattgatc
    17341 agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta ccggataacg ccagatgccg
    17401 tcagcatcgg cgaagcgcca tagcggcgta ccatcctgcg ccacgaccac tcgtgcggga
    17461 ttgacttcat gcagcggtag aggccagagt ttatctgctc cccacgctgc aagaaaaagg
    17521 agaaatggcg cagctgccag cgttatccag cagccgcgtt tggttaacaa gcgaggcatt
    17581 tacggtcgga caatcagcag atcttcagcc gcgccggtcg cccgccattg gggaacatac
    17641 attgattcca ccatcggttg cggtacctga tacgttcccg gcgtcaccgc ccgcgccaga
    17701 tacaccagcg ttaccggttg gtattcatca acggcaaccg ccgccacaaa gcgatcgtca
    17761 cggaactcaa tgtgcttaat actcgcctgc tgcatctggt tcagtaagtt ttgcacttcg
    17821 ccaccacttt gctccaggct ggcgctaccg ttcgccagat tctgattttc cagttccaga
    17881 cccgcaggca gcagatccac gactaacgca tccggcacgc tgttactggc ttttacctgc
    17941 aaccagacca gcaccagatc gccgctacgt aacgagtcca gcgatttgct cttaccatca
    18001 gtaccaagaa tatgacgctc gatttgcagc acattgttcg ccggtaaagg tgcggattgc
    18061 ggataaccgc tggcatccac acgcagccat aacggctgat cgccactgtt tctcacctgc
    18121 aaggtggcaa gttgatcgct attcagattg ctgtttagcg ttttctcgcc tgtcagcggc
    18181 tcagctgaga aagaggtttg cgcctgccat ttacccggta aatcctgaat cgtacgggca
    18241 gccaggaaca acgcgttact ttcctgcgtc gatagccagc gttcaccaaa tgcctgctgc
    18301 gaaagcgtgt tcagcaaagt gttctgctca tcgggtagca gtttattttc ttccagcaag
    18361 tagagcatta acgcgttgtc gcgcagtgga ctaccgtaat cacccagcca tatccgctca
    18421 tcactatttc tcggcgtttt cagcgccagc acaatcgctt cttcaccacg cgttgcatca
    18481 cccatggttt tcagcgcaac gccaagttgc agcagcggta aaccagaagc ggcatctgca
    18541 cgatgctccc agatttcacg cagcgcaccc agtggtgctt tttgctgacg ggccagcacc
    18601 agcgcagcgt aagactgaac ggcgaattta ctggctttga gattatccgc gtacgggatc
    18661 gacatcatgc ccggatcttg taaatagcgc agcagacgct cattaccccg gttaatggcg
    18721 tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca ggaaatccat cacgtaagcc
    18781 gtcagccagt actcttcgtc accgttttta tcccacagcg caaagccgcc gttatcacgt
    18841 tgcatttgca gcaaacggga aatgccgata tcgaccgatg cacggcgttt ctcatcactg
    18901 tcgcctttga tgcccaacgc ctgcagttgg gctgcgttgg tataaagtga cgggaacagg
    18961 ccgctggcgg tttgctcaag acagccgtac ggatacgctt ttaactcttt gatataacgt
    19021 gcaatgttca gcggtggttt gccgctcaac aacaattgcc cttccagcgt aacaggcgag
    19081 aagttttgca atccatccgc cggaatcgcc catgtctcac caggctgtaa cgccgtaccg
    19141 taattaaccg tttgtgccgg gaacgccgga cggacgccga ttttccactg cttatgctga
    19201 tcggcaacgg tttcacccgg taacgctaac ccgctaatgg tggcctgaat ttctccatcg
    19261 ccataacccg gcaatgctcg caccgggata aacagcgtag tacgcacacc tggcgctaat
    19321 tcaacggctg cgggtgaatc gctgaccagt tcaagcaaac cactggcggt cagggcaacg
    19381 ttcagttttt gcggtttatc ggtaagatta gtgatatcca gcgtcagacg cgaggtatcg
    19441 ccactcgcca taaagcgcgg catgttcagt tcagcaatca ccggtgcggc aactatcact
    19501 ttgctttcgt tgctaccgaa atcatctgcc gtccaggctt gcgccatgac gcgcaattca
    19561 ccgttaaaat cgccaatcgg cagtgtaacc gagccttcgc cctgttcgtt gagcgttacc
    19621 ggcagcgcct gctgcgcgac aatattgacg tgattgaccg gcggtttacc accacgtttc
    19681 agctcatcac catcgccacc gaaacgcaga gctgccagac gcccctgacc ttcaataacc
    19741 tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct gaccaaagaa cgcctgccac
    19801 ggatctggcg tgacgtagtc agtaatattc agcacaccac tatcaacggc agacaccagc
    19861 acattcacct gtttaggctt ctcgccattt ttagtgctgg ctttaatttt cacggttaat
    19921 ggttgattgg gacgcatttt tgctggtgtt tccagcgcca gatcgaggcg acggttttca
    19981 tcgccaagcg gcagatgcaa cacaccaacc gcgcgttttg gcgtcgcgga gcgagattta
    20041 tcgccaggac gtaccaccag cgtacttaaa tacagatcat gacgattcca ggttttatcg
    20101 accggaatcg tcagatccag cccttgagcc ggaacatcaa tctcttgcca ccacagcggc
    20161 ccttcactgg actcgaccat cgcataacct ttacccgccg ttggcgcggc gatatgcaac
    20221 ttaatggtgt cgccagggcg ataactggct ttatccagtt tcagcgtgac acggtcgggt
    20281 cgcactgcgc cgctaccgtc gctgttgtcc tgccagctat agccagccca gaaacgaaca
    20341 ctactgaccg cttcattcgg cgctttgact tccagacgat aagcgcccca ctctaccgga
    20401 aaactgacct tgccggtttc gtccgctttc agatccagag tttgttcatt ttcgagcaga
    20461 tctttttgat caaactgtga ctgccagcct tcatcttctg accagttcca gtagtaatcg
    20521 cggcgttcgc gaatcaggcg cacctgcaag cccgacacgg cttttttcac gccttgcgca
    20581 tcgctataaa cgatgtcaaa agcggcgtta ctgccttcat caacaatggg ctgtttcacc
    20641 gtgctgtcag tacgataatc gtaaaccgat ttcgaggcga actgcggacg gatccccggc
    20701 aatgcatcgg caggccagat agcctgctca gcgcggcgcg tcaccgggcg accgcccgat
    20761 tccagcaaac taccctggaa aataacctgt aatggggaat gcgtttcctt ccactggctt
    20821 tctgtagaaa cttcgccgcg ccctttatca tccagcgtca actgaacttc atccagcgtg
    20881 cgggaaagat tttcggcagc tatatcgccg aattcaaaac caggtaaggc tgacacagct
    20941 tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat taccattagc aggtgcacca
    21001 tacaggtagt accccaccac ggagaatttc acttcatctt tcggcgttag cggggttttc
    21061 tcaccggtca gattcagcgc catgcgctct ggcataaaat cttcgacgtg gaaatcccac
    21121 atccgatact ggttatcgcc cgtgttagcg cgaatatgcc acataccggt tgccgcattg
    21181 ctatcgagtg gccaggtaaa gtggtagagg ccattctccg gctgactaac gacgctcctg
    21241 agtacctgcc catcgggttt aatcacgtct aacttgatgg gttgattggg caacgcttta
    21301 ccgtctgcat cacgcagcaa accattgagg attaccgttt cacctgggcg ataaagatcg
    21361 cgtgggccaa acatgaaaaa ctgtttgcta tagcctggcg cgccagcaat gttaaattct
    21421 gctaagtcca gcgccggaag ttttaaatcg agtagcgttg tctgaccgtc tttacgcgcc
    21481 aacagtaatg ccgcgttttt atcattttcc agctgcacat gcccctgagc gtcactggtt
    21541 gcctgagtca gagtctgccc tttctcattt aataaagaga cttcaattcc ttgctgggcc
    21601 gcgccgtttt ccagactttg ggtaaagata tccagacgat tgtgataacg gtgagctgaa
    21661 acgccgatat cacttaacgt aaacagcgtc gcgggattac tgtaatcgta acgtccagcc
    21721 tgattcatca cagccagata cacgcccgcc tgctgaagcg gtttgatatc gcccagcggc
    21781 agcaataatt tttcacgggt gttacgcgca ggattgagat caaaccgtcc ggtgtagacc
    21841 agatccgcca tctgcagcag tttgtctgac tgccagttcg ccagcgaatt gcggtattcc
    21901 cattggctaa tgaatgctgg cagagattct ggcttaacgc ggaagaagtt aacatcgaca
    21961 ttattaacgt tgagcgccat taccggcagc ccttcaacga ctttgccagg cagcagcgaa
    22021 ccacggctgg caaaaccgac gctgggttgg atgtcgcggg tagttatagt tttttcgtaa
    22081 tctttactga aggttgcgtt gttgagtgct ttgacctcct tgccaatagt aacgatcaaa
    22141 tcacgtttcg gttcgaggtg gcgtaaacgt aactctttaa gattatctga cagctcccag
    22201 gcaccatcca ctttgccgct ttttttatcg acgacatgaa taacgcgtga gaaatcctga
    22261 tccgggtcga gagggatgga gaacgtcagc accagcgtgg ctgcaccatc aagttggact
    22321 tcagagagat ccagcaatgt cagcacctta ccggcactct gttgggccag tttttgtctc
    22381 tccggcacgg agagttttgc tgaactcgcg ttttctgaag aggccgcttt agtaacttca
    22441 gaaggcgcat cttttttcac cgctgttggc gcgttatcgt tgttgtcgca ccctgccagc
    22501 gccagcatta gcatgcaggc ggctacgcgt aactttttca tttttcatcc ctgccacaat
    22561 ggcccgttag caacgtcgaa taattattat gcgtgagaat ttacgttatg taattcagta
    22621 tcacgcccat acaatccgaa ttttagtgag aattgtggct cagacagcca aatcagcctc
    22681 tttcgcttgt cgcttttcac taaacatccg acaatttagc ctaaccccgg caaaaatgga
    22741 gatgcctatg tccacgacat ggtttgtagg agccgactgg ctcgccgaac atattgatga
    22801 cccggaaatt cagattattg atgcccgcat ggcgtcgcct ggacaggaag atcgtaacgt
    22861 tgctcaggag tatctgaatg gacatattcc cggcgcagtg ttttttgata tcgaagcgct
    22921 ttctgatcac acttccccgc ttccgcacat gctgccgcgc ccggaaacgt tcgccgtggc
    22981 gatgcgtgaa ttaggcgtta accaggataa gcacctgatt gtctatgacg aaggtaatct
    23041 tttctcagcc ccacgagcat ggtggatgct gcgcaccttt ggtgtagaga aagtgtcgat
    23101 tctggggggt ggacttgcag gctggcagcg cgatgatctg ctgttagaag aaggtgcagt
    23161 agagctgccg gaaggagagt ttaacgccgc gtttaatcct gaagccgtgg tgaaagtaac
    23221 cgatgtatta ttggcaagcc atgaaaatac ggcgcaaatt attgatgccc gcccggctgc
    23281 acgttttaac gcagaagttg atgaacctcg cccaggttta cgtcgcggac atattcccgg
    23341 agcactgaat gttccgtgga cggaactggt gcgcgaaggc gaactaaaaa cgaccgatga
    23401 actggatgcg atattttttg gtcgcggcgt cagctacgac aaaccaatta tcgtcagctg
    23461 cggctctggt gtaacggcag ccgtggtttt gttagcactc gcgacgctgg atgtgccaaa
    23521 cgtgaaactg tacgacggcg catggagtga atggggcgcg cgggcagatt taccggttga
    23581 gccagtgaaa taagtatttt acaggcaata aaaaaccgcc gaatttggcg gttttttatt
    23641 gctggtctgg ttcgcggcct ttccagcagg ttgtattacc gtagtaatgc aagcgcgtct
    23701 cagcggagac aatactcgcc agtaactctc tttttgtcaa gcaaaagaga gttattattg
    23761 ttctgttagt gtattatcca ctgcggccct ttccgccgtc tcgcaagcgg gcgctggctt
    23821 aaggaaagga tgttccgcga ccgttagagc aagcgcgtct cagcgcttgc tatcgcggca
    23881 atatcgccag tggtgctgtc gtgatgcggt cttcgcatgg accgcacaat gaagatacgg
    23941 tgcttctgta tcgtacttat tgtttctggt gcgctgtttt cccaagtaaa taataaccgg
    24001 agtctctccg gcgacaattt actggtggtt aacaaccttc agagcagcaa gtaagcccga
    24061 atgccgccct ttggggcggc atattttaga ttatccgatt ctgtttaaag tcacgcaaaa
    24121 aaccacccca gcgacgttca tagaatggca caatatgttc ggtaataaag tggctaattc
    24181 ctttttcccc ttttttcacc tgacaaatat cgattggttc atcgccaggt aatgtatcgg
    24241 tcgctacact tcccgtcgcc tgaataattt cttcgatatc accatcggct tcaatgccaa
    24301 taagtaaatt aggctgtgcc tcttcgttct ctttaattga acaaataaaa gcacgcttca
    24361 ccgacttaat ggttttaaat aaggtggtga gtgaatcaat catttgtgct ggcggctctg
    24421 cgacttccga taatatcagc gattcaccgc cttccaggat ttcctggctg ctcagcggat
    24481 ttccctcttc accaatcaac aaactgattt cacgcggcat aaattcttta ccggtcggca
    24541 gtttggcatt aaggaagagc gtttcgccaa gggtcatctc aaacaatgtg cgaacgggca
    24601 ttacgacaaa tgcctgttcg tcttcaaccg cctgttgaag cgcttctaac gaggtgaaaa
    24661 aaggaatgac gctggtgccg tcttcttttt cccagtgctg taaatcaagc gcactatctt
    24721 caaccacagc ctcgccctgc gccgccgtac caggcaccca gacggtggat tccagtagag
    24781 tacggaaaaa ggccgggcgg tgcgccggtt cggttgctgc tttttccagc aggtcttcaa
    24841 gttcgttttt tgtttcggac ataagaacca caattcattc aacgttcggg gcgcaaatgc
    24901 ctgatgcgct acgcttatca ggcctacaag ggattcgcaa tttgttgaat ttgcagaatt
    24961 tgtaggccgg ataaggcgtt tacgccgcat ccggcatatt agtttacgcc gttaacagat
    25021 tagcaatcgt gcgcacacca agtcccgtag cgcccgcaga ccactgttca accggcgctt
    25081 tacggtaagt cgccgagcag tcgatatgca gccagccttg ctgatagttc tcaacaaagt
    25141 gcgacaggaa gcccgccgcc gtgctcgcgc ctgccggata cgccgcgctt ccggtattgt
    25201 tcagttcggc aaagttagac ggcagctggc tgcggtggaa ttccgccagc ggcagacgcc
    25261 agaacggttc gttctcttgt gaggcactcg ccagcaaacg accggcaagc gcatcgtcaa
    25321 aactgaacag cgcgtgataa tcattaccca gcgcagtttt cgccgccccg gtgagggtcg
    25381 ccgcatcaat gatcagttcc ggtttctgcg cactggcatc aatcagacca tcggccagca
    25441 ccagacgccc ttcggcatca gtgttcatca cttcaacttt tttaccgttg cgataggtga
    25501 tgatatcgcc cagcttgaac gcattgccgc taatcaggtt atccgcacag cagaggaaca
    25561 gcttcacgcg cttgttcagt ccgcgcgtaa tggcaaatgc cagcgccccg gtaaccgttg
    25621 ccgcgccgcc catgtccgac ttcatcgagt ccataaacgc agtctgtttt atgctgtagc
    25681 cgccggagtc aaaagtgata cctttaccta ccaggcacgc gtacaccggt gcttctttat
    25741 cgccagttgg gttgtaatcc agcgccagca ataccggaga acgttctgaa ccgcgcccga
    25801 cagtgtgcag ccccatataa ccttgctcac gcagatcttc gcctttggtg atccgataag
    25861 tcacacgatc gcccgcgacg ttgctgatca gatcaacagc acgctgtgcc agttgcgatg
    25921 gtcccaattc ttctgccggt gcgttgatgg tgtcacgcac ccagtcgatg atcatcaggc
    25981 ggttatccag ttcctggcgc tgggcatcgt ccagatccgg ccactccact ttacgcgtgc
    26041 ctttcggggc tttgtaacct tgccagaatg cccagcagcg atccgcatcc cagccttcac
    26101 cgcttaactg aacatgcttg atgcccagac cgtcaatctt gcgcgccgca cgctggatca
    26161 gccccagatc gtctgccccg ttcaaatgca gggtaatgcc gtcattatta atgctgtaag
    26221 ttgctttttc tccccagcgc gcgtcggcag gttgggtaga gagggtaatc ttcatcgctt
    26281 ctgtcatttt agttatcctt ctttgtaaaa gggccgccag ttggcagccc caggattttt
    26341 ccgctaaatg attcgcgttg caggaaggcg gcaagtgagt gaagccccag gagcatagat
    26401 aactatgtga ctggggtgaa cgagcgcagc caacgcatct gcggcgtgaa gcatgacgcg
    26461 gaaattattc ggcctcgtcc agccagacta acaaaatcgc ttcgaggatt ttctcgttgg
    26521 atgcctgcgg gtcgtcgtcg aaatcttcca aatcgcaaat ccactgatgc atatcggtga
    26581 atcgaaccgt tttcggatca agatcgggat acgcatcgta cagtgcttcg ccaatttcgc
    26641 ggctatcggt ccacttaagt cccatactaa cctctgttaa tgctcacgcg catggttgat
    26701 agtgtaacgc gggatttcga ccactaaatc ttcgtcggtg acgcgcgcct ggcagcttaa
    26761 acggctttcc ggctccagtc cccaggcttt gtccagcatg tcgtcttcct gctctgagct
    26821 ttccggcagc gagtcaaaac cttcacgaac gatgcagtgg caggtggtgc aagcacagga
    26881 tttttcacag gcgtgttcaa tctcgatacc gttacgcaga gctgcgtcga gaatggtttc
    26941 accgctatta gcttccagaa cagcgccatc agggcagaga tcctgatgag gcaaaataac
    27001 aatctttggc atattaaacc tcgtccacgg aatggccttt cagcgcacga cgaaccgact
    27061 ggtccatgcg gcgagcggcg aaatcctggg tttgtttgtc tacgttttta atcgcttgtt
    27121 cgatggcgtc aacgtcatcg ccctgcgcca cttcactcag gtgagcggca gcatcgtcaa
    27181 tgacctgacg ttctgcggcg cttaacagcg cggcatcagc agccagcgcg ccgtgcagac
    27241 tttccagcac acgcgccgct tctacttttt gttctgccag cattcgggct tttacgtcct
    27301 gctcggcata gctcattgag tctttgatca tcgaagcgat ttcgctatcg gtcagaccgt
    27361 aagacggttt gacctgaata gacgcctcaa cgccggtgga tttctccatc gccgtcacgc
    27421 tcaaaagacc gtcggcatcg acctggaacg tcacgcgaat atgcgcacca ccagccggta
    27481 gcgccggaat accacgcagc gcaaaacgcg ccagtgagcg gcagtcctgc accagttcgc
    27541 gctcaccctg cattacatgg atagacatcg ccgtctgacc atctttaaag gtggtgaaat
    27601 cctgagcgcg ggccaccgga atcgtggtat tacgcggaat cactttctcc accaggccgc
    27661 ccatcgtttc gaggcccagc gacagtggga tcacatcaag cagcagcatt tcgctgtccg
    27721 gcttgttacc caccagaata tccgcctgaa tcgccgcgcc aatagcgacg actttatccg
    27781 ggtcgatgga agtcagcggt ggacgaccga aaaattcgcc tacccgttca cgcaccagcg
    27841 gcacgcgagt agaaccgccc accatcacca cttccagcac ttcatcagct tctacacccg
    27901 cgtctttcag cgcgcgacga caagccagta aggttcgttt taccagtggc gcgatcagtt
    27961 cattgaattg ttcacggctg atttcgccct gccagcccgc aacgttaacg gtcacggagt
    28021 ccgcatcgct cagcgcgatt ttggctgcaa tggcggcatc cagcagttca cgctgaacgc
    28081 ggttatcgct acgatcagga atgcccgcct gctcgcgaat gtaatccgcc agcagatggt
    28141 cgaaatcatc gccgccgagc gcggaatcac cgccggttgc cagcacttca aacacgccgc
    28201 gacttaagcg cagaatggaa atatcaaacg tcccgccacc gaggtcataa acagcgatca
    28261 cgccttcctg accggaatcc agcccgtagg cgatagccgc agcggtcggt tcgttaagta
    28321 agcgcagaac atgcaacccc gccagacgcg ccgcgtcttt ggtgccctga cgctgggcat
    28381 cgtcaaagta cgccggaacg gtgataacta caccatccag ctcgcctgcc agggcttcag
    28441 ttgcccgcgc cgccagtgct ttgaggatgt ccgcagaaac gcgcaccggg ttcagcagcc
    28501 ccgccgccgt ttcaatcata ggcaggccgt tttcgctggc ctggaattga taaggcagat
    28561 gcggatagcg ttgctggata tcagccagcg agcgccccat caggcgttta acagaactaa
    28621 tggtgttggc ggtatcaagc gctgcgttag tacgcgcgtc ataacccacc gaatgccctt
    28681 gctgttgata gtgaacaaca gatggcagca ggtgacggcc ttcatggtcg gctaacgttt
    28741 cggcctgacc gctgcgcact gtcgccacca gcgagttggt tgtgcccagg tcaataccag
    28801 ccgccagacg acgctgatgc ggcgcagcac tcaaaccagg ttcactaatt tgtaataagg
    28861 ccatgtttag cttccagaaa ttaaaaatcg agcagttttt cttcgagttg ttcggcactg
    28921 ctccgcagtt tatcgagaaa acgcagctta cgcacggtat ccgccgccgc gtcccacgcc
    28981 tcgttgtcta actgttcaac catcaactga tggcgggtat caaacatctt tttcacacgt
    29041 ttgataaagc tttccagccg cgcttcatct ttcgcctgtt cgatctcgtc cagctcttcg
    29101 cgcagctcca actgttccat caggaacgcg gtgtcgcgca cagtatgctg ctcgctggcg
    29161 agatcaaaac cgtgtaaaga aagcaaatat tccgcgcgca ttaacggatg acgcagcgtt
    29221 tgccaggcct ggttaatggt tgcagattgc tgtacggcgg cgagttgttc cgcctggctt
    29281 ccgctggcga atttatcagg atgatactga cgttgtagat cctgaaaacg caggctcagc
    29341 gcctgggtat cgagttgata acgggcaggc aagccaaaga gggtgaagta atccataaca
    29401 ttctcagggc tgcgatccgc cgcgataacg gcgaatcagg taaaacaaac cccacgcgca
    29461 ggcgaccacg gtggggttat cggtatgcgc atcaaacgtg gaagctttcg ccgcaaccac
    29521 actcatcttt gacgttcggg ttggtgaatt taaacccttc gttcaggcct tcttttacga
    29581 agtccagctg cgtaccgtcc agaaattgca ggcttttgcc atcgaccacg actttcacgc
    29641 ctttgtcttc aaacacgatg tcttccggcg tcggttcgtc aacaaattcc agtacataag
    29701 ccatacctga acatccggag gttctcacgc ccagacgcag gccaaaccct ttgccgcggt
    29761 tagccagaaa ggtatttact cgcgctgctg cactgtcgct cagtgtaatc gacataacca
    29821 aacctcaact cttattttgc ttcacgtttg cttttatagt ccgcaatggc ggctttgatc
    29881 gcgtcttctg ccagaataga acagtgaatt ttcaccggcg gcagttcaag ttcttcagca
    29941 atatcggtat ttttgatcgc ctgcgcttcg tcgagagact tccctttcac ccattcggtg
    30001 accagggagc tggaagcgat agcagaaccg cagccgtaag ttttaaaacg cgcgtcttca
    30061 atgatacctt catcgttgac tttaatctgc aacttcatca cgtcgccaca ggccggtgcc
    30121 cccaccatgc cgctgccgac gttctcgtcg ttgttgtcaa aggaacccac gttacgcgga
    30181 ttctcgtaat ggtcgataac tttttcgctg taagccatta taaattctcc tgattccgat
    30241 accgattaat gatgagccca ttcgatgctg ttcagatcca cgccctgctt gtacatttcc
    30301 cacagcggag aaaggtcacg cagacgaccg atggatttac gaactaactc gatggtgtag
    30361 tcgatctctt cttcagtagt aaaacgacct aaagagaaac ggatagagct atgtgccagc
    30421 tcgtcgttca gccccagcgc gcgcagcacg taggacggtt cgaggcttgc tgacgtacag
    30481 gcggaacctg aagaaactgc gaggtctttc agcgccataa tcagcgactc accttcaacg
    30541 tagttgaagc tgacgttgag aatgttcggc gcaccgtgtt ccaggtcacc gttcaggtaa
    30601 acttcttcga tatctttgat gccgttccac agacggttac gcaggccgcg cagacgttcc
    30661 atctcggtcg ccatctcttc ttttgcgatg cgataggcct cgcccattcc gacgatctgg
    30721 tgaacaggca gagtgccgga acgcataccg cgctcgtgac cgccgccgtg catttgcgct
    30781 tcgatgcgta cgcgcggttt acgacgtaca tacagcgcac cgataccttt cgggccatag
    30841 attttgtgac cggagaaaga catcaggtca actttcaact ggctcaggtc gataggcagt
    30901 ttgcccacgc tctgggttgc atcaacgtga tagataatgc cacgagcacg gcacatttcg
    30961 ccgatagccg cgatatcctg caccacgccg atttcgttat ttacgtgcat gatggaaaca
    31021 aggatggtgt cgtcacgcat cgctgcttca agttctttca ggtcgataat gccgttacgc
    31081 tgcggtgcca ggtaggtgac ttcaaaacct tcgcgctcca gctgacggca ggtatccagt
    31141 accgctttgt gttcggtttt gctggtgatg atgtgcttgc cttttttctg ataaaagttg
    31201 gctgcacctt tgatcgccag gttgtcagat tcggttgcac cagaggtaaa gacgatttca
    31261 cgcggatcag cgccgaccag atcggcaatc tgattacggg cgatatctac cgcttcttca
    31321 gcctgccagc cgaaacggtg agaacgagag gccgggttac caaaggttcc gtccatcgtc
    31381 ataaactgca tcattttctc ggcaacacgc gggtccaccg gcgtggttgc ggagtagtcg
    31441 agataaatcg gtaatttcat tgctctataa actccgtaca tcactcaatg caaggaacca
    31501 ggctaccggc tggatgtacg accgtgttta cggagcgcga tacgccccgg cctgattctg
    31561 aattcttttt attaagcgcg taacttaacg tcgatcgcgt cttgtatgcg ggtgcgtggc
    31621 gcgtcgtgag tatgctgacg accagacaca tccagcactt cctggttatt aaccagttcg
    31681 cctaaagtaa tgttgttgag aaaaccggtg agacggtcgc tcaaatcacg ccacagcgcg
    31741 tgggtcaggc atttatcgcc gccctggcag ccgcctttac cctgacaacg ggtggcatct
    31801 acagattcgt caacggcgct aatcacttcg ccaacggcga tgctgctggc atctttgcct
    31861 aacagataac caccgcctgg tccacgtacg ctggaaacca gaccattttt acgcagacgg
    31921 gaaaacagtt gttccagata agaaagggaa attccctgac gttcggaaat atcagccaac
    31981 ggtaccgggc ccgcttcaga gttgagcgca acgtcaagca ttgcggtcac ggcatagcgc
    32041 cctttagatg tcagtctcat gtcttacttc acctcaaact cgcccctgcc cggggttttt
    32101 tattgtaaaa gtgggggtat tgcatagcag ggtcaagtct gacattcccg agtaaattgg
    32161 tcaactattt acttgactga tttagtcggg tatttaacct tcagtgccat ttttttatcg
    32221 tggtatttgt acctgttgtc ggatgcggcg tgaacgcctt atccgaccta cgattgcgtg
    32281 cacttccagc acttgtaggc ctgataagac gcgttaagcg tcgcatcagg caaccactgt
    32341 tattcggcct tattaccttt attctgctgc tctatagaag ccagaatccc gcgcaggata
    32401 ttcaattcct ggctttccgg gcgcgcacgg gtaaacagac ggcgcaattt attcatcacc
    32461 tgccccggat ggttttcacg gataaaaccg gttgccagca gcgtttgttc gagatgacca
    32521 taaaaacgct ccagatcatc gaccagcgga tacggcgtct cttcatgctc gacctgctcg
    32581 ccattttcct gagtcgccag ccaggccata cgcacttcat aggcaataac ctgaaccgcc
    32641 atcgccaggt tcagcgagct gtattccggg ttcgcggcaa tcgcgacatg ataatggcat
    32701 ttctgcaact cttcattggt caagccgacg cgctcacgac caaacaccag cgccaccggg
    32761 gtatttgccg cttcagcgac gcttttcaga ccgcattcgc gcgggtcgag catcggccac
    32821 ggcagcgtgc gggagcgtgc gctggtgccc accaccaggc tacaaccggc taacgcttcg
    32881 tcgagcgtat cgacgatgtg agcattacca atcacatcgc tggcccctgc tgccagggca
    32941 atcgcctggg agtcgggttt caccagtgga ttaaccagcc acagattggt taatcccatt
    33001 gttttcatgg cacgggcaac agaacccata ttgccggtgt gtgacgtctc caccagcaca
    33061 attcgaatat tttgcagcat tgtctttctt catctaaaga ttattcacgc atcttatcat
    33121 aaaacgaaga cagatgccga tctcgctgct atactctgcg ccgttttccc gttctttaac
    33181 atccagtgag agagaccgat gcatccgatg ctgaacatcg ccgtgcgcgc agcgcgcaag
    33241 gcgggtaatt taattgccaa aaactatgaa accccggacg ctgtagaagc gagccagaaa
    33301 ggcagtaacg atttcgtgac caacgtagat aaagctgccg aagcggtgat tatcgacacg
    33361 attcgtaaat cttacccaca gcacaccatc atcaccgaag aaagcggtga acttgaaggt
    33421 actgatcagg atgttcaatg ggttatcgat ccactggatg gtactaccaa ctttatcaaa
    33481 cgtctgccgc acttcgcggt atctatcgcc gttcgtatca aaggccgcac cgaagttgct
    33541 gtggtatacg atcctatgcg taacgaactg ttcaccgcca ctcgcggtca gggcgcacag
    33601 ctgaacggct accgtctgcg cggtagcacc gctcgcgatc tcgacggtac cattctggcg
    33661 accggcttcc cgttcaaagc aaaacagtac gccactacct acatcaacat cgtcggcaaa
    33721 ctgttcaacg aatgtgcaga cttccgtcgt accggttctg cggcgctgga tctggcttac
    33781 gtcgctgcgg gtcgtgttga cggtttcttt gaaatcggtc tgcgtccgtg ggatttcgcg
    33841 gcaggcgagc tgctggttcg tgaagcgggc ggcatcgtca gcgacttcac cggtggtcat
    33901 aactatatgc tgaccggtaa catcgttgct ggtaacccac gtgttgtgaa agccatgctg
    33961 gcgaacatgc gtgacgagtt aagcgacgct ctgaagcgtt aatgactcag gcgggtgatg
    34021 tcactcaccc gccctcgcct ttcaggcgct attctgaaat acttcctcac cgctttactt
    34081 tctttcgtca ctctcccacc atctttcccc gattaatgga tgataaagaa gtcgcaagga
    34141 aaggttatgg cactgccagt gaacaaacgc gttcccaaaa ttctgtttat tctctttgtt
    34201 gttgccttct gcgtttattt agtgccgcgc gttgccatca acttcttcta ttatcctgac
    34261 gataaaattt acggtcccga cccctggtcg gcggaatccg tcgaatttac ggctaaggac
    34321 ggtactcgtc tgcaaggctg gtttattcct tcttcgacgg gccctgctga caacgccatc
    34381 gcaaccatca ttcatgctca cggcaatgcc ggaaatatgt ccgcccactg gccgctggtc
    34441 agttggttac ctgagcgtaa tttcaacgtt tttatgtttg attatcgcgg gtttggtaaa
    34501 tcaaaaggca cgccgtccca agccggattg ctggacgata cgcaaagtgc catcaatgtg
    34561 gtgcgccatc gcagtgatgt aaacccacaa cgtctggtgc tgttcgggca gagtattggc
    34621 ggggcgaata ttctggatgt tattggtcgg ggtgatcgtg aaggcatacg tgcggtgatc
    34681 ctcgactcca catttgcctc ttatgcaacc atcgccaacc agatgatccc cggcagtggc
    34741 tacttacttg atgagagtta cagcggcgaa aattatatcg ccagcgtcag cccgatcccg
    34801 cttttactca ttcacggtaa agctgatcac gttatcccat ggcagcacag cgaaaagttg
    34861 tatagcctgg caaaagagcc aaaacggttg atcctaatcc cggatggcga acacattgat
    34921 gctttttccg atcgtcacgg cgatgtttat cgcgaacaga tggtggactt tatccttagc
    34981 gcgttgaatc cacagaacta acccatgatc gctagcacga taatcattca caaaaccacc
    35041 ttaagacatg ctaatccact ggtcagaaca gtttaagatg agaaaaattc tgtgacgctt
    35101 gccaacattt ctgatgatta gcattccctt cgccatttcc ctgagcaaac tttagctatt
    35161 cttatcaatt atgcttatgg gagatctaca gatgatgcct acgcttgctc caccatctgt
    35221 cctttcggct ccccagcgcc gctgccagat cttgctgacg ctctttcagc cggggttgac
    35281 cgccaccacg gcaactttca gcgagcttaa tggtgtggat gatgatattg ccagtcttga
    35341 tatcagcgaa acaggacagg agatcctgcg ctatcatcaa ctcacactga cggctggtta
    35401 tgacggtagc taccgggttg aaggtacagt gcttaaccaa cgtttgtgtt tatttcactg
    35461 gctacgacgt ggtttccgtc tgtgtccatc atttattacc agccatttca cccccgccct
    35521 gaagagtgaa ctgaagcggc gcggaattgc gcgtaacttt tacgacgata ccaatctaca
    35581 agcgttagtg aatctctgct cccgacgact gcaaaaacgc tttgaaacgc gcgatattca
    35641 tttcctgtgt ctgtatctgc aatattgttt gctgcaacac cacgctggaa ttacgcctca
    35701 gtttaatccg ctccaacgtc gctgggcgga atcctgcctt gaatttcagg tagcgcagga
    35761 aattggacgc cactggcagc gtcgggcgct ccagcctgta ccgcctgatg agccactgtt
    35821 tatggcatta cttttttcca tgttgcgggt tcccgatcca ttgcgtgatg cgcatcagcg
    35881 agacagacaa ttgcgtcagt ctatcaaacg tctggtaaac cattttcgtg agctgggaaa
    35941 tgttcgtttt tatgatgaac aggggttatg cgatcagctt tatacccacc tcgcccaggc
    36001 gttaaatcgc agtttttttg ccatcggtat tgataatacc ctgccggaag agttcgccag
    36061 actgtaccca cgcctggtgc gcaccacccg cgcggcgctg gccggatttg aaagtgaata
    36121 cggcgtccat ctttctgatg aggaaagtgg tctggtcgcg gtgattttcg gtgcctggct
    36181 aatgcaggaa aacgatctgc atgaaaaaca gattattcta ctgaccggga atgatagcga
    36241 gcgagaagcg cagattgagc agcagttacg cgaactaacg ttactgccgc tcaacattaa
    36301 gcatatgtcg gtaaaggcat ttttgcagac aggcgctccg cgtggcgcgg cactgattat
    36361 tgcgccttat accatgccat taccgctctt ttcaccaccg ctgatctata cggacctgac
    36421 gctgacaaca catcaacagg agcagatccg caaaatgctc gaatcagcat gagggaacaa
    36481 ctttcgggcg caaaaacatt gccggcagcg ccaccagcgc cattacccag aacacgccgt
    36541 ggcccagata ttgatacagg aaaccggcga aaacggtcat gatagcgata ctgccgccca
    36601 tcgcgacggc agagtaaacc gcctgtaaac ggatgacttc gctaccctgg cgagcagcaa
    36661 tataacgcat ggcggccagg tggcagaccg tgaaggtgcc gcaatgcaga atttgcacca
    36721 ctatcaacca cggcaacgcc gtagttgctc ccataatgcc ccagcgcact acgccgcaaa
    36781 tcgccgagat caacagcata tcgcgtgcac tacaacggcg gaaaagttta ttactcagcg
    36841 caaagataat gacttccgcc accacgccca gcgaccacaa ataccccacc gccgaggccg
    36901 agtagccagc tgcctgccag taaatggcgc taaaaccgta ataggccgca tgtgccccct
    36961 gcaataaaca aacgcaggcc agaaagcgcc agttctggcg aaccagcgcc aaccacgctg
    37021 accaaccggt gctctcctgc tggcggcttg ccccttgtgg ctgaatcgtc ggacggatga
    37081 gaaagccgag cagcatggat gccacgccca acgtcaacag cgcgaggatc acccgataat
    37141 caaacatagt gaccagtttg cccgtcagcg ccgagccaat gacaaacgcc accgagcccc
    37201 acagtcgcac tttgccgtaa tcaagcggga actgcttttg ccacgtattc gccagtgcat
    37261 cggtcaacgg taccagcggt gagaaaaaga ggttaaagcc aatcatcacc agcatcagcc
    37321 acgctacgtg cgcccccgcc cagaaggcga cagcaaagag aagtgtcagc agtgccagca
    37381 cgcgcaaggc ggaaatcagg cgggaaggat cgctgacgcg gggcgcgatg agcaaactcc
    37441 cgaggaaacg ggcaaccaga cctgccccca ataacaggcc gatggtttct ggcgttaaac
    37501 caatcccttt aagccagacg ctccagaaag gtagaaaaat gccgtaacta aaaaagtatg
    37561 tgaaatagcc gagcgccaac cagcgcgtgg attgcaaaac catgagtccc tcccgtcagg
    37621 aggcgttagt ctggggttaa tcgaatgttg gtgcaagttg aaaaataaga atattcgagc
    37681 ctgcttcccg atgtctgtaa cgccagttca caagcactaa aaaactgaga tccgcgacgc
    37741 atgttgcaaa aataatgcgt aattatgccg ttacgcttgc caaacgttcc tgcacaatgg
    37801 cgatgaaatc gcgtaatgcc ggcttcatct cccctttctt ccacgccatc agtaaagcaa
    37861 tggaaggcac atttccggca atgggacgaa aaacaacctg tccggtatta aaattattca
    37921 tataaccggg tatcaaagtg acgcccagcc ccatgcccac cagattcatg gtcaccagaa
    37981 tattcgttgc cacctggacg atatttggct ggctgttttc ttgcgcaaac cacgctttaa
    38041 cgatcggcgc aagcgaaccg gaatacaccg gatcggtact gacgaaattc acgccatcca
    38101 gttgcgcggc ggtgatctct ttttcatgtg ctaaagggtg atcaaccggt aacacaacca
    38161 ctaatggttc gtcaaaaagc tccagataat caatctccgg gctataaaca ggatggcgca
    38221 ttaagccgac gtcgagttca ccacggcgaa ttttttcctc ctgttgcgtg gtgattaaac
    38281 tcaccagctc aatcaaggtg tctggctgtc tgagacgaaa catcggtaat acttttggca
    38341 gtaaattcac ttccgccgat ggcacaaagc caatggttaa ttgtctgtct tcctgaacaa
    38401 ttttccgcgc ccgtaatttg gcattttccg cttgttcgag gattgccagc gcgtcctgga
    38461 gaaaacattc tcccgccgcc gtcaacgcga ctttgcgctt atccctcacc agtaacggaa
    38521 caccgacaca gttttcaaga tcgcggatct ggctgcttaa cgaaggctgt gaggtatgca
    38581 gtttttccgc cgcacgggta aagttcagtg cctgcgccac tgcgacgaaa tagcgtaaat
    38641 gccgtagttc catcaccttc cccttgttat cgaaaaaacg tctcagctgg tagaaattaa
    38701 ctatttcaca tattagcaac caaccagcaa catccttatg gcacaaaaat agaaggtcaa
    38761 tacatcttat ctttcaggat taaaaaatat gaccacaccc tcagatttga acatttacca
    38821 actgattgac acccaaaatg gtcgggtcac tccgcgtatt tataccgacc cggatattta
    38881 ccaactggag cttgagcgta ttttcggtcg ttgctggcta tttctcgccc acgaaagcca
    38941 gatcccaaaa cccggtgatt tctttaacac ctacatggga gaagatgcgg ttgtcgtagt
    39001 gcgtcagaaa gacggcagca tcaaggcgtt tctcaaccaa tgccgccacc gggccatgcg
    39061 tgtgagttat gcagattgcg gcaacactcg cgcctttacc tgcccgtatc acggctggtc
    39121 ttatggcatt aacggcgagt tgatcgatgt accgctggaa cctcgcgcct acccacaagg
    39181 gttgtgtaaa tcccactggg gactaaacga agttccttgt gtggagagtt ataaagggct
    39241 gatttttggc aactgggata ccagcgcacc gggcctgcgt gattacctgg gtgacattgc
    39301 ctggtatctg gatggcatgc tggatcgtcg cgaaggcggc accgaaattg tcggcggcgt
    39361 acaaaagtgg gtgatcaact gtaactggaa attcccggca gagcagttcg ccagtgacca
    39421 gtatcatgct ctgttcagcc atgcttctgc cgttcaggta ttaggggcga aagatgatgg
    39481 cagcgataag cgcctcggtg atggacaaac cgcccgcccg gtgtgggaaa ccgccaaaga
    39541 tgcgctgcaa tttggtcagg acggtcacgg tagcggtttc ttctttactg aaaaaccgga
    39601 tgctaatgtc tgggtcgatg gcgcagtttc aagctattac cgcgaaacct atgccgaagc
    39661 agaacaacgt ttaggtgaag ttcgcgccct gcgcctggcg ggtcataaca atatattccc
    39721 cacgctttca tggctaaacg gcactgccac actccgcgtc tggcatccgc gcggccctga
    39781 tcaagttgaa gtgtgggcgt tctgtattac tgacaaagcc gcctccgatg aagttaaagc
    39841 cgcttttgaa aacagcgcca ctcgtgcttt tggtcctgct ggttttctcg agcaggatga
    39901 ctcggagaac tggtgtgaaa tccagaaatt gcttaaaggc caccgcgccc gcaacagcaa
    39961 actgtgtctg gaaatggggc ttggtcagga aaagcgccgc gacgacggca ttcctggcat
    40021 tactaactat atcttttcag aaactgccgc tcgtggaatg taccaacgct gggccgatct
    40081 tctgagtagc gaaagctggc aggaagtgct cgataaaacc gccgcttacc agcaggaggt
    40141 gatgaaatga gtgcgcaagt ttcactagag ttacatcacc gcattagcca gtttctcttt
    40201 cacgaagcca gcttactgga cgactggaaa tttcgtgact ggctggcgca gctcgacgaa
    40261 gagattcgtt acaccatgcg caccacagtt aacgcgcaaa cacgcgaccg ccgcaaaggc
    40321 gtccagccac cgacaacctg gatttttaat gacaccaaag accagctgga gcggcgaatc
    40381 gcccgtctgg aaacgggcat ggcatgggca gaagagccgc cgtcacgcac ccgccactta
    40441 atcagcaact gccagataag cgaaaccgac atcccaaacg tatttgctgt tcgggtgaat
    40501 tatctgcttt atcgggcaca aaaagagcgc gatgaaacgt tctatgtcgg gactcgtttt
    40561 gacaaagttc gccgtctgga agatgacaac tggcgcttgc tggaacggga tatcgtcctg
    40621 gatcaagcgg taatcacttc ccataacctg agtgtactgt tctgatgaat cgaatttatg
    40681 cgtgtcccgt tgcggatgtg ccggagggtg aggctctccg gatcgatacc tcgcccgtca
    40741 tcgccctgtt caacgttggc ggcgagtttt atgccattaa cgatcgttgc agccatggta
    40801 atgcgtcaat gtcagaaggg tatctggaag atgacgccac ggtggagtgc ccgctacacg
    40861 ccgccagttt ttgcctgaaa acggggaaag cgttatgcct gcccgccacc gatccgctca
    40921 ccacttatcc agtacacgtt gaaggtggtg acattttcat cgacttaccg gaggcgcagc
    40981 catgagcgat ctgcataacg agtccatttt tattaccggc ggcggatcgg gattagggct
    41041 ggcgctggtc gagcgattta tcgaagaagg cgcgcaggtt gccacgctgg aactgtcggc
    41101 ggcaaaagtc gccagtctgc gtcagcgatt tggcgaacat attctggcgg tggaaggtaa
    41161 cgtgacctgt tatgccgatt atcaacgcgc ggtcgatcag atcctgactc gttccggcaa
    41221 gctggattgt tttatcggca atgcaggcat ctgggatcac aatgcctcac tggttaatac
    41281 tcccgcagag acgctcgaaa ccggcttcca cgagctgttt aacgtcaatg ttctcggtta
    41341 cctgctgggc gcaaaagcct gcgctccggc gttaatcgcc agtgaaggca gcatgatttt
    41401 cacactgtca aatgccgcct ggtatcctgg cggcggtggc ccgctgtaca ccgccagtaa
    41461 acatgccgca accggactta ttcgccaact ggcttatgaa ctggcaccga aagtgcgggt
    41521 gaatggcgtc ggcccgtgtg gtatggccag cgacctgcgc ggcccacagg cgctcgggca
    41581 aagtgaaacc tcgataatgc agtctctgac gccggagaaa attgccgcca ttttaccgct
    41641 gcaatttttc ccgcaaccgg cggattttac gggtccgtat gtgatgttgg catcgcggcg
    41701 caataatcgc gcattaagcg gtgtgatgat caacgctgat gcgggtttag cgattcgcgg
    41761 cattcgccac gtagcggctg ggctggatct ttaaggaagc acgatgaaag aaaaaaccat
    41821 cattattgtc ggtggcgggc aagcggcggc aatggctgcg gcctcgctac gccagcaagg
    41881 gttcaccggt gagctgcatc tgttttccga tgagcgacat cttccttatg aacgacctcc
    41941 actctcgaaa tccatgttgc tggaagattc cccgcagtta cagcaggtgt tacccgctaa
    42001 ctggtggcag gaaaacaatg ttcatctgca ttccggtgta accatcaaaa cgctgggtcg
    42061 cgacacacga gagttagtgt taaccaacgg cgaaagctgg cactgggatc agctttttat
    42121 agcaaccggc gcggcagctc gaccgctgcc gttgcttgat gcactgggag aacgctgctt
    42181 taccctacgc catgccggtg atgccgccag actgcgagaa gttctgcagc ccgaacggtc
    42241 agtcgtgatt gtcggtgccg gaactattgg tctggaactg gctgccagcg ccacgcagcg
    42301 cagatgtaag gtgacagtga ttgaactggc ggcaaccgtc atgggccgta atgcaccacc
    42361 gcccgtgcaa cgctatcttt tacagcgcca ccagcaggct ggtgtgcgca ttctgctcaa
    42421 taatgccatt gaacatgttg tcgatggtga aaaagtagaa ctgacgctgc aaagtgggga
    42481 aacgcttcag gctgatgtgg tgatttacgg tattggtatc agcgccaacg agcaactggc
    42541 tcgcgaggcc aaccttgata ctgccaatgg cattgtcatt gatgaggctt gccgcacctg
    42601 cgatcccgcg atctttgccg gtggcgatgt ggcaatcact cgtcttgata atggtgcact
    42661 acaccgctgc gaaagctggg aaaacgccaa taaccaggcg caaattgccg ctgccgcaat
    42721 gttagggcta ccgctaccgc tactgccgcc gccgtggttc tggagcgatc agtacagtga
    42781 taacttacag tttattggcg atatgcgtgg cgatgactgg ctttgtcgtg gcaacccgga
    42841 aactcagaag gcgatttggt ttaatctgca aaacggcgtg cttatcggtg cggtcacgct
    42901 gaatcagggg cgtgagattc gcccaattcg caaatggatc cagagcggca aaacgtttga
    42961 tgcgaaactg ctgatagatg agaacatcgc gcttaaatca ctgtaaccag gataattagc
    43021 gaatatctca atgcctgggg cgtggcgagg tgcaagagtg tgtattacgt ttaaatcaca
    43081 ttatcttgca aagggattgg ttatgaacac actacgttat tttgattttg gagctgcccg
    43141 ccccgttttg ttattaattg cccgtatcgc cgtggtctta attttcatta tttttggttt
    43201 tcccaaaatg atgggctttg acggtacggt ccaatatatg gcctcgttgg gcgcgccaat
    43261 gccgatgctg gcagcgatta ttgcggtagt tatggaagtg cccgccgcga tattaatcgt
    43321 gcttggcttt ttcacccgtc cgctggcggt gctgtttatt ttctacacgc tgggtacggc
    43381 ggtgattggt caccattact gggatatgac cggcgatgcg gttgggccaa atatgattaa
    43441 tttctggaag aatgtcagta tcgctggcgc attcctgctg ctggcaatta ccgggccggg
    43501 ggcaatttct ctcgatcggc gttaggaaag atgccggatg cggcgtgaac gccttatccg
    43561 gcatttaata aattacagcc actctacccg caacgacatc tctgaagttg ttgattcccc
    43621 tggtgctagc gcaatgagat cacccccttc cgggcgatga tgatcatccg gcgcatggct
    43681 catcggttcc agacagaaga aatcaaacgc atatccttta tcaaacgcag ggtcggaaac
    43741 aaagatgaaa taacacggtg caggtggcgt cgtttccatg atgatggcat aaccttcctg
    43801 cggctgctcg atgcgggcct gaccattcca tccggcaaaa ccattgttca cccactggcg
    43861 cggcaacggc gcgggctggt taaaatccag ttcctgcggt agctgctcgc aaaactcacc
    43921 cgccagccac tgctcccgct ccagccagta accgctcgcc tgtgcctgaa tccgcgtttg
    43981 cggcgacaac ggaaaataag gatgccagcc ggtaccaaat ggcagcgtct ctgccccttg
    44041 attggtgaca gagagcgtca ccgtcagcgt atccgccgtt aaatgaaacg cctgacttac
    44101 ccgatagtga tagacaccgc tgcgatgttc atacaccaga cacaaactat catcgctgtg
    44161 cgagacacat tgccactcgc ccagccagcc atcgccgtgt agatagtgcg catcccactc
    44221 aacattcggt tgcagttgat actcacgccc ctgccagaca aaacgattgc cgctcacccg
    44281 gtttgcaaat ggcaccagcg gaaaacatga ggcatcggtt gccacaccgc tttttttacc
    44341 aggacgtaag agcggcgtcg tatcgcgcca gaagccttcg attacgccgc cctggtcaga
    44401 aacgtccagc tttagcgacc cgtgggataa ggtatagatg gtcatccgct aactccttaa
    44461 tccggggagt taatcacaac tttcccgcat ttgccgctcg ccatcagcgc ataggcatca
    44521 cctgcctgtt ccagcgagaa gcgatgggta atggcgttac gcggccacag cttccagtcc
    44581 gtcagatcat gggcgcattt ttccatatgg aacagactgg tcacccagga gccgataatc
    44641 cgccgttgat ggtgcatcag atcggcgctg acctcgaatt ccacttttcc ggtttcacca
    44701 atgtaaacca cccgtcccca gtcagcggtg gattgcagtg ccagcaagcg acctgcggca
    44761 ttaccggaac aatcgagcgc aacatccgcg ccaccgtggg tgagttcggc gataatctgc
    44821 ggcagacctt cggtggttgc taaatagccg tgatccatca cccctaactg ttttgccatc
    44881 gccagacgtt ccggcagcat atcaacgccg atgatccgtt ttgcaccgcg acctttcgcc
    44941 agcatcatcg ccatcatgcc gaccggcccc agaccgacca ccagcacttt atcgctgccg
    45001 gaaacttcgc cgcgcaaaat cccttcatag gccgtgccaa cgccgcaact gataaacgca
    45061 ccatcttcgt agctcagcgc atccggcagg aggatcagat ctttttcttc cgccagcaga
    45121 tattcggcat gaccgccgtc acgctgccag ccgtaagccg cttttccttt gccagtacaa
    45181 gagataggaa agccgcggcg gcagttcggg caaaaaccac agccagaaat gtgatacacc
    45241 agcacgcgat caccctcttt aaaatggcga cagccttgcc ccatcgccac aatctgcccg
    45301 cacggttcat gaccgttgat aaatccctgg tataacggtt tatcgggtgc cgccgccgtg
    45361 gcacggtgtt gatgatagat atagtggaca tcgcttccgc aaatcccgga ggatttcatt
    45421 ttgatcagta cctggttaat ccccggcgtc ggcaccgcaa cttcccgcag atcgacggtc
    45481 gaatttcctg gtaaataagc tgccagcatc gttttcataa atccctcatt aacaatacga
    45541 ttaattttca tccctgccgc acccgcgcca ggggcgttaa ggtttagcgt ttcgctttac
    45601 tgctacgctg ggtcagcaag atattcgcca gcaccgccac cacgatgatg acgccgcgca
    45661 ccacctgctg gaaaaaggag ttaatcccga gcagcaccag accgttaccg attagcgtaa
    45721 tcaccagcac accaagcaat gtaccgaaca gggaaccgcg accgccggaa agtgccgtac
    45781 cgccgaccac gaccgcggcg atgacgtcaa actccagacc gtttgcggca cctgcgttac
    45841 cagaaccgag gcgcgccgcc aacaaaatgc cggtcaccgc cgctaataat cccgaaaggg
    45901 taaagataag aatgcgcacc cgacgaacgt tgatgccgca caactgcgcc gccgtagcat
    45961 taccgccaac ggcaaaaacc gagcgcccga aggcggtttt gcggctgatg aacacaaaca
    46021 gcgcaaacaa cacaatcatg atcagcgcgg ataccggcac accgagaaat tgtccgccca
    46081 gccagtccag cacctcgttt tcgtcaatcg gcactggcag tgcgttcgtc ataaacagcc
    46141 ccattccgcg cagggcgctc cacagcccca gtgtggcaac gaaacttggt acgttaaaca
    46201 cgccgcgcag caccccggcc agcgttccca tcagcgcgcc taacagcaac accagcagac
    46261 acgccaccgc cagcggaact tcaaattgca gcaaaaatgc caggcacacc gagacaaaag
    46321 ccaccatcgg cccaacgctg acatcaattt caccggagat aataatcagc gtcatcgccc
    46381 aggcggcaat cccaatggtg gcggcatcgc gcagcacgtt catctggtta ttcaatgaga
    46441 taaagccaag cgcgttcagg gagaagacca gataaagaat ggcgattacc accagcaaac
    46501 cgatctcatt aatatggcga ctgacaaatt gtttgagcga gacgctcttg ccctgcggca
    46561 atgataatga cgaagcagac atcttcaggt tcctcggtaa aattcatcag tgcacagaca
    46621 gaatggcgga catcagctca tccacattga ccggcgagtg aaactcctgc gagaacgtac
    46681 cgtgctgtaa caacaggatg cggtcgcaca ccagcggcaa ctcctccact tcactggaga
    46741 taaacaccac gctttttcct tcggcagcca gctcacggac aatacggtaa atctgctgtt
    46801 tggcttcgat atcgacgccg cgcgttggct cgtcgagcaa caaaatctgg ctggcggcat
    46861 agacccaacg accgatcacc actttttgct gattgccgcc agaaagcgtg ccgatgggcg
    46921 tttcgctact ggcggccttg accgtcatcc gctgcatcac ctcttcggtc aggcggcgga
    46981 tggtggacca ttgcagtacg ccgttggcgc tgattttttg ccgattggtc agcactgtat
    47041 tttcgtcaac gcccaaccag ggaatgatcc ccgcttcttt gcggttttct ggcgtatagc
    47101 caatgccgcg tttcagcatg tcgccgtaat cggggcgcgt gattttctcg ccgttgataa
    47161 caatttcgcc ctgttcatac tcctccagcc caacaatcgc cttcagcaat tcactgcgcc
    47221 ctgcccccag caggccagca atgccaagca cttcgccgcg acgtaacgta aagctgatat
    47281 cctccagctt gggcttatgg cgtaatgcac ggacttccag cacggcctga tcgacaattt
    47341 cctgaggggc taccggcgca atatcaacgt gatcgcgccc tagcattagc gacacaatat
    47401 gatgcgtgga ggtgttttcg agcatcacat cgcccgccac ctgaccatcg cgcataacgg
    47461 tggcacagga ggcaatgcgg cgaatttctt ccatccggtg gctgacataa atcaccgcca
    47521 cgcccagtgc cgacatcttt ttcaccgcgc tgatcaccag ttcaacttcc gcactggcaa
    47581 gcgaactcgt aggttcatca agaatgacca cgcgcggctc gcccttcatc acccgtgcaa
    47641 tttccaccag ttgcttttgc gccgggctta gcgttgaaac aagttgttca ggactaacgt
    47701 caacgcccag cgcctgtaag caacgttggg catcctgcgc catttgcagg taatcaatca
    47761 tgccgttgcg gcgaggccat tgaccaaggc agaggttttc tgccaccgtc agcccttcca
    47821 ccagacttaa ttcctgataa accgcacgaa cccccagttc agcggcacgg cgagtcaacg
    47881 tagcttcgtc accttccagt cgcgtctcgc caatccagat atcaccgcta tccgggcgtt
    47941 cgctgccggt aagcattcga atgagagtcg atttgcccgc gccgttttta cctaacagcg
    48001 cacgaacttc gcctttattg agcgtgaagt taacgttatc cagcgcaacg acgccggggt
    48061 aacgcttatt tcctgccacc acttttgcta ccgggactgc ctctgttgcc gtgaacatag
    48121 ggacctctgc gaatcagcga ttagggcaga ccatcaacgt gcgttgccag ccactgttta
    48181 ccatcttccg ttttggtata gagatcgatt ggcacctgaa tcactttttc accgtcggct
    48241 tgtttattga taaccttcaa tgtttgcgcg agaacagcat tgcccatttt cttaccggaa
    48301 atatccacta ccgctttcag cacctgattg ttttccagct cctgagcaat ttcagtggtc
    48361 atatccgaac cgaaaacagc aatttttccg gcctgatttt gattacgtac cgcttttacc
    48421 gcgccgagtg tcgcaccgcc cgactccccc ataatggcgt tgagatccgg cgtggagata
    48481 atcagttttt caccaacgga aatcgcttta tctaaaacag tcccttcctg attagcgaca
    48541 attttcgcgc cgggaacgcg ggattttaat acttcttcaa atcctttacg tcgctgcaca
    48601 caaacttcaa aggcttcgca attgatgacg gcaattttcg gctggtcaat tttattggca
    48661 ataaaataat cggcggcagc gttacccagt tttttaccaa attccagcgg atcgccgacc
    48721 agatacgccg agacatattt atcgcccccc ttttgattaa tacaggtgtt gtagcaaatc
    48781 accggaatac ccgcttcact ggcgcgacga acggtacggc tactgccatt ttcagacact
    48841 gccgataaaa taatggcatc gacattacgc gccacgaggg tatcaacaaa ggtactttct
    48901 ttcgaaatat caccctgggc gttagtttca attaactgta cctgtactga agaatctttt
    48961 gccgcatcct gaacgccctg acgcactcca gcgtaatatc cctgagtatc aaggtatatt
    49021 gcgccaatgg tcatttcttt ttccgcagcc ctggcaaata gtgcgctacc taatagcgta
    49081 gccattaata atagattacg ggttgttctg atttttgtag gcatagagcc tcctgtaggg
    49141 tttttattaa caacggctta ttctaattat tttgtgatga gcggcagcgc gtgccgctca
    49201 aatattacag ttaatagggt ttagtgaacg tagttaaaaa taaaaggcat cacggtggtg
    49261 aataatgccg ctttcggcca ggccggatta atcgccgtca attcagcgcg agcagactca
    49321 tattgcgcaa cctccccgag tcccgcggac gccagcatcg ccaccatcag gcatttttct
    49381 ttatgctgct gttgtaaatc gccatacagc gataacaggt caggttgtga aacagcaaag
    49441 aaatccgcct caatactggt tttcgccatc tcttgcgccc actgtttcat ttcactaaac
    49501 agttgctgtg cggtgtgttg ttcgcccagc agtcgcagcg ccattccttg ccagaagaga
    49561 taatcaaccg gctgatcgtt gtaataactg tggatgttaa tggtgcgatc gccggtcgcc
    49621 gccagacgta aacaacgcat cgcttcagtt tcatcgccct gcgcgttggc gcatatcgcc
    49681 tgccagaacc agatgtcgtt atcagtttgc cccggtaaac ggccttcgct taaattctcc
    49741 ggataatgca gcgcggcatg aagcagttcg ctggcctgct gcggctgtct ggcatcaagg
    49801 tgctgccagg cgcgtaataa ctggttgagg ataaactgac tggtgacctt cccttccccg
    49861 ccttcccacg ggtggaattt gcgcgtggcg agaatgtccg ccgctttgtc tgcctgaccc
    49921 gtgagatgcc acaaattgag caactctgct gtcatgtcgt cgcgtttcag cgcaatttcc
    49981 agattatttt ccagacgcgc cagtcgtttc tccggtgtgg ctccgcttaa cttatcaagc
    50041 aaatcccgtt cgaaaagcag acgtgcatcc tgcggcgcaa gctgataagc attatcaaga
    50101 taacgcgcgg ccagctcata atcgtgttgc ttattccacg catggatcgc taacccgcgc
    50161 cagccgtcgg caaactccgg cgacatctct acgcaacgtt gccataaggt aatggctttg
    50221 ttgtagctac gcttgttgta gtagaagcag gccagtaaat gacgggcaaa ccagcactct
    50281 tcaatactct ccagcgccgc cacttcttcc agcgtattcg ggaaacggac aaactgcggg
    50341 aagacatcaa tggcttttgc gaccagttcg ccgcgttcgg ctttcggcaa taaactcgcc
    50401 tgcaggtaaa gcggcagcgt gcgctggtag tccagtgcgt tcagcatctc tgccgccagg
    50461 gtgggcattc cccagttaat cagttgcccg gcggtcatca gagcgttaac gtcgcgtccc
    50521 tgacacagac cgcgccactg cgcgagggct gattcgctac gaccatcgaa ccagttcagc
    50581 caccacagag tggcgttcag cggataatca cgcagcagtt tcgcgcgctg caaacgcgcg
    50641 ttgtcctgac gaccacttaa caccagcagc agattatgca ggcaaagcac ttcctgattg
    50701 gttgggcagg cacgaagact ttgttggcaa aaatccagac cagcgtcgaa gttaccatta
    50761 cgcgccgcca gtcgtgccag gccataatag ccaccggctt tgctgttgcc gctccagacc
    50821 gcacgccaga aatcctcttc ggcttgttga tattgtccct gacgttcgta agcactggcg
    50881 cgaatcaaac tggcctgtcc gcactgcgga tttttgttca gcgcatgtgc gcgtttcagc
    50941 gcctgagtgg catacgccac cgcttgcggg aaatctgcgc ggttatattc cagcatcgcc
    51001 agcgccaggt tacagcggta atccagcgga tccagcacca cgccgcgcag gtagtaatcg
    51061 aacggtgaac ggctggcgtg atgatattgc tccagatgct gaccgataaa ccaggcttca
    51121 tctgtactgg taatgtcttg tgctgcgagt ggcgctttgg cgacgtccgg caacggcaac
    51181 tcttgcggct gatgttcctg ataactcagg acaatattgc cgtcggcatc ggagagctca
    51241 atggtcagcc tttcaggatt gataccgtgc aacacgccct ggatggcggt cgcaggcatc
    51301 agtgccacgg catcatcaag taacgcgttg catttgccga tttcgcggat cgccaggcgg
    51361 tatccgttca acggagagat ggcatacagc ccccactcaa tcccccgctc actacgctgg
    51421 agttttatca ccgcatcgcg ggaggcattt tgcaccatgc ccaaagaatg ataaggcagg
    51481 aaatactgct caaaacgctt ctcttcgtaa gcatcaagcc aggtaaaatc aggctggtta
    51541 tcggcaaaaa taccggtcat cagttcgata tacgggccgt tattgtcggt cagactcttg
    51601 tcccacgcct ggccgaattc actgtgtccc cagctccact gttttttacc tggcgcgata
    51661 tggtggttgg caacgtgtag caaaccgcca tcttcatcgt gacaccacgc gccaacaaaa
    51721 tcgtactgtg atttttcagc catatatgag gttggaacgg gcacattttt atagcgagaa
    51781 atgtccactc cggcggagta gtccactttg tagtaagtgc cggtggcgat ggggaaagcg
    51841 gagacggccc gtttgccgtg atcaaacacc gcagtcacat ccggtgggaa aacgctctga
    51901 tgcccttccc cccctttcac tgccgggttg gcccaccaca agaaatgacg cggcgtggcg
    51961 ttaccgttat agacgcggct ggcgatttcc agcgccgccc ggtcagggcg cagggtgaaa
    52021 cctgtcatca cctgtaaacc atgcatcggc tccgtttcgc ctacccacac cgtctgtgca
    52081 ccgtcttcat gggcttcgag ggtgaaatca acgggcataa aggtggtcgg gcgatgatgt
    52141 tgcggccagt taaactcaat cccaccggaa atccacggcc ccagcagccc caccagcgca
    52201 ggtttaatga cttcattgtg ataaacaaaa tcgcgctgtt tcactttatc ccacgcgcga
    52261 tgcacccgac cgcccagttc cggcaggatc atcactttga tgtagtcgtt ttccagccac
    52321 accgcctgcc aggatttcag agttttctgc tcgctcagcg tatcggtcac gccgtaagga
    52381 taaaccgcgc cggacgatcc ctgataaacg cgattttcca ggaacatggg atgtatatcc
    52441 tgcggcccgg tttcataggt cgggatctca acgcgctctt gccacacttt tactggagtc
    52501 atggtgccct caatattaac aagacatact gaattaaaag atttgtggca gtgtattgaa
    52561 caatctggca atgttttcgc ggaataatca cgcaattaac taaacaaggt ttagtgaaga
    52621 tgagagcctg cattaacaat caacagattc gccaccataa caaatgcgtg attctggaac
    52681 tgctgtaccg gcaaaagcgc gccaataaat caacgctggc ccggctggcg caaatttcga
    52741 ttccggcagt cagtaatatt ttgcaggaac tggaaagcga aaaacgggtg gtgaatattg
    52801 acgatgaaag ccagacgcgc gggcatagta gcggtacatg gctgattgcg ccggaaggtg
    52861 actggacgct gtgcctgaac gtaacgccca ccagtattga gtgtcaggtt gctaatgctt
    52921 gtttaagtcc gaaaggtgaa tttgagtatt tacagattga tgcaccgacg ccgcaggcgc
    52981 tgctgtccga aatcgaaaaa tgctggcatc gtcatcgcaa actgtggccg ggccgcacca
    53041 tcaatctggc gctggcaatc cacgggcagg ttgatccggt gaccggcgta tcgcaaacca
    53101 tgccgcaagc gccgtggaca acgccggttg aggtgaagta tctgctggaa gagaagctgg
    53161 gcattcgggt gatggtcgat aatgactgcg tgatgctggc gctggcggag aaatggcaaa
    53221 ataattcgca ggaacgggat ttctgcgtga tcaacgttga ttacggcatt ggctcgtcgt
    53281 tcgtgattaa cgagcaaatt tatcgcggca gtttgtatgg tagcggacag attggtcaca
    53341 ccatcgttaa tccggatggc gtcgtctgcg actgtggacg ttacggctgc ctggaaaccg
    53401 tcgcctcgtt aagtgcatta aaaaaacagg cgcgggtatg gctaaaatca caaccggtta
    53461 atactcagct tgatcctgaa aaactgacaa ccgcgcagtt aatcgctgcc tggcagagtg
    53521 gagaaccgtg gatcaccagc tgggttgatc gctctgccaa tgccattggt ttgagtctgt
    53581 ataacttcct caacatcctc aatattaatc agatttggtt gtacggtcgc agttgtgcct
    53641 ttggtgagaa ctggcttaat actattattc gccagacagg atttaacccg ttcgaccgcg
    53701 acgaaggacc gagcgtgaaa gcgacgcaaa ttggctttgg gcaattaagc cgcgcacaac
    53761 aggtgctggg aattggctat ttgtatgttg aggcgcagtt acgacagatt tgatggcgcg
    53821 ataacgtaga aaggcttccc gaaggaagcc ttttttcaac agattaaccg tttcgcttat
    53881 gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac ctttgatgcg ctcgataacg
    53941 gcttcatcat tgatgctgtc cagcacgtca cacatccagc cagccagttc tttcgcttct
    54001 gcttctttga agccgcgacg cgtaattgcc ggagtaccca cgcgaatacc ggaggtcaca
    54061 aacgggctct tcggatcgtt cggtacgctg tttttgttga cggtgatgtt agcacggccc
    54121 agagcggcgt ctgcttcttt accggtcagg tttttatcga ccagatcaac caggaacaga
    54181 tggttatcag taccgccgga aaccactttg tagccgcgtt ccaggaacac ttctaccatc
    54241 gctttagcgt ttttagcgac ctgctgctgg taagttttga actcaggctc catcgcttct
    54301 ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg gaccgccctg accaccaggg
    54361 aaaacggcag agttcagttt tttgtacagc tcttcgctac cacctttcgc caggatcagg
    54421 ccgccgcgcg gacccgccag ggttttgtga gtggtggtag taacgacgtg agcatgagga
    54481 accgggttcg ggtagacgcc agcagcaacc aggcccgcaa cgtgcgccat atcaacgaac
    54541 aggtaagcac cgatgctgtc agcgatttca cgcattttcg cccagtccac cacgccggaa
    54601 tatgcagaga aaccaccgat aatcattttc ggcttgtgtt ctttggcttg tttttccaga
    54661 tcggcgtagt cgatatgacc ggtagcatcg ataccgtaag gaacgatgtt gtacagttta
    54721 ccggagaagt taaccggaga accgtgagtc aggtgaccgc catgcgccag gttcataccc
    54781 agaacggtat cacctggttc cagcagcgcg gtgtagaccg caaagttagc ctgggagccg
    54841 gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt ctttcgcacg atcgatcgcc
    54901 agttgttcaa cgatatcaac atactcgcaa ccgccgtagt agcgtttgcc cggataacct
    54961 tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca ttacgcgcgg gctggtgtag
    55021 ttttcggagg cgatcagttc gatgtgctct tcctgacgta ctttttcctg ctccatagcc
    55081 tgccacagtt cggcatcata atcggcaatg ttcatttcac gctttaacat ccgcatctcc
    55141 tgactcagct aacaataaaa tttttggcct ttataggcgg tcctgttgga caacggcgaa
    55201 cagtataacc gaatcattgt gcgataacag gtcttgacaa aggaatttac gcaaacgatt
    55261 accttcaggc tacgcaaggc tttggagaat aaagagcttg caaccggaaa cggatttctt
    55321 ttcaggtttg tgatgcaaat ttttcacttc atcacattct ttctgaaaaa caccaaagaa
    55381 ccatttacat tgcagggcta ttttttataa gatgcatttg agatacatca attaagatgc
    55441 aaaaaaagga agaccatatg cttgacgctc aaaccatcgc tacagtaaaa gccaccatcc
    55501 ctttactggt ggaaacgggg ccaaagttaa ccgcccattt ctacgaccgt atgtttactc
    55561 ataacccaga actcaaagaa atttttaaca tgagtaacca gcgtaatggc gatcaacgtg
    55621 aagccctgtt taacgctatt gccgcctacg ccagtaatat tgaaaacctg cctgcgctgc
    55681 tgccagcggt agaaaaaatc gcgcagaagc acaccagctt ccagatcaaa ccggaacagt
    55741 acaacatcgt cggtgaacac ctgttggcaa cgctggacga aatgttcagc ccggggcagg
    55801 aagtgctgga cgcgtggggt aaagcctatg gtgtactggc taatgtattt atcaatcgcg
    55861 aggcggaaat ctataacgaa aacgccagca aagccggtgg ttgggaaggt actcgcgatt
    55921 tccgcattgt ggctaaaaca ccgcgcagcg cgcttatcac cagcttcgaa ctggagccgg
    55981 tcgacggtgg cgcagtggca gaataccgtc cggggcaata tctcggcgtc tggctgaagc
    56041 cggaaggttt cccgcatcag gaaattcgtc agtactcttt gactcgcaaa ccggatggca
    56101 aaggctatcg tattgcggtg aaacgcgaag agggtgggca ggtatccaac tggttgcaca
    56161 atcacgccaa tgttggcgat gtcgtgaaac tggttgctcc ggcaggtgat ttctttatgg
    56221 ctgtcgcaga tgacacacca gtgacgttaa tctctgccgg tgttggtcaa acgccaatgc
    56281 tggcaatgct cgacacgctg gcaaaagcag gccacacagc acaagtgaac tggttccatg
    56341 cggcagaaaa tggcgatgtt cacgcctttg ccgatgaagt taaggaactg gggcagtcac
    56401 tgccgcgctt taccgcgcac acctggtatc gtcagccgag cgaagccgat cgcgctaaag
    56461 gtcagtttga tagcgaaggt ctgatggatt tgagcaaact ggaaggtgcg ttcagcgatc
    56521 cgacaatgca gttctatctc tgcggcccgg ttggcttcat gcagtttacc gcgaaacagt
    56581 tagtggatct gggcgtgaag caggaaaaca ttcattacga atgctttggc ccgcataagg
    56641 tgctgtaatt tgatgttgcc ggatggaaac atccggcaac ccttgacgcg gtttaaattg
    56701 ccgcgtcgtc ctcttcaccg gtacggatgc gaatgacccg tgccacgtca aagacgaaga
    56761 ttttaccgtc accgattttg ccggtttgcg ccgtgcgaat aatggtatcg acacaggtat
    56821 cgacaatgtc gtccggtacg acaatctcaa ttttcacttt cggcagaaaa tccaccatat
    56881 actccgcgcc gcggtacagc tcggtatggc ctttctggcg accaaagcct ttcacttcgg
    56941 tcaccgtcat gccggtaata ccgacttcgg ccagtgcttc gcggacatcg tccagcttga
    57001 agggttttat aatcgcatca atctttttca tgctattcct tgaaaaggtc gcctgtcttt
    57061 tgatctgcta aacgtaacac ataacgccaa ttcattcctt gaaatcgttt gcatccagct
    57121 cgtgtcggga aagcagttta taaaattctg tccggttgcg ccccgccatt ctcgccgcgt
    57181 gggtgacgtt gcctttggta atttgcagca gcttacgcaa atagttgagt tcaaactgat
    57241 tacgtgcctc aacaaaggtt ggcagcgccg tgttttcacc ctccagcgcc tgctctacta
    57301 atgcatcgct aataaccgga gatgaggtca gcgccacgca ctgttcaatc acgttgacca
    57361 actggcgcac attacccggc cagctcgcgg tcatcaggcg tttcatcgca tcagtggaga
    57421 acgcgcggac aaacggttta tgtcgctctg ccgcctggcg caacaggtga tttgccagaa
    57481 gcggaatgtc ttctgtgcgc tctgccagag ccggaatttt caggctgaca acgttgaggc
    57541 ggtaatagag atcttcacgg aattccccgc gcgccatcgc ttttggcagg tcgcggtgag
    57601 tggcggaaat aatccgcacg tcgatatcaa tatcgcggtt gctgcccagc gggcgcactt
    57661 tacgctcctg caacacgcgc agcagtttga cctgcaatgg tgcgggcata tcgccaatct
    57721 catcgagaaa tagcgtaccg ccttccgccg cctggaataa accttcgcga ttgctgacag
    57781 cgccagtaaa cgcgccacgc gcatgaccaa acagctccga ctccagcaac tgctcgggca
    57841 atgcgccaca gttaatagca ataaatggtt tgctgttgcg cgggctggcg ttgtggatag
    57901 cctgggcgaa aatctctttc ccggtgccgc tctggccatt aatcaacacg ctgacgtctg
    57961 attgcgccac cagccgcgcc tgttccagca aacgcagcat cagcgggctg cgggtgacaa
    58021 tcgcctcgcg ccagcgttcg tcggtggctg gcgcggattg ttccagcgca tcgtcaattg
    58081 cctgatatag cgcatcttta tcgacaggct tggtgaggaa actgaacacg ccctgctgtg
    58141 ttgcagcaac ggcatcggga atagaaccat gcgcggtaag aataattact ggcattcccg
    58201 gctgcacttt ctgaatttca gcaaacagct gcataccgtc catttcatcc atccgcaggt
    58261 cgctgatgac taaatctact ttttcgcgat tcagtacccg taatccttca gcgccacttt
    58321 ccgccgtgac cacgctgtag ccttcgctgg tcaggcgcag gccaagcagt ttcagcaatc
    58381 ccggatcgtc atcgaccaat aataaatgcg caggtttatg gctcatcagg agtgacctca
    58441 tgggtggatg gcgcgggctt ttcaccctcg cgcggcgtat ccggcgagaa atttccagcc
    58501 ggtttgcggg tcgagagctg gcgttcaata tcagtcaggt tttccagctt gcgggtggtg
    58561 agttccagtt gctgctgtag aacgtgatgt tgctggcgca atgtatccag ctcgctgtcg
    58621 ctggcctgct ggagcttgcc gtagcgttgg cgctcttccg ccagttgcag ttgcagcgcc
    58681 tgaccgtcgc gccagagttg atacagaggg cgaacctgtg ccgggatctc ggtacttagc
    58741 gcatcaatgc gcgcgaccag ctggcggcgc tcatacggcg taattttggc gtcggcgagc
    58801 aaaatccctt gtttaaaggt attttgccag ctgccgtcgt catattggcg agcttgctga
    58861 cgtgactgcg caggcattaa acgatcagca caatccatcg cccgcagcca gtaaagcgga
    58921 ttggtttcgg ttgatttacc ttgcagcgcc cagatgtcgc tacattcagt agaaagatag
    58981 tcagccagtt gataaaccgg aattttttct tctgctagcg tatcaatggc tggcttattg
    59041 tgactctgca cgcaacccag caatgccaga catggcagcc ctgccagcca cagccgtcgg
    59101 ggcaataatc gttgaaaaat gtgtcgcata ttcaccagac ttaaagccta tcccagtggg
    59161 cgtaattgtt gcagacagtc tggacatgga cagcgcggag aaaccggagc gtacatatcg
    59221 tacgtgaaga tttcgagcac tgcccggggc cgaactgaca aataaaatag cctataggat
    59281 aggctgttag cattatttcg tgtttttcga cgacggtaat tcaatgcgga aacaaacgtc
    59341 ttgcccgctc tcgtcgacca gatacagttc cccttgcata cggcgaatac aatccctggc
    59401 aatgcttaat cccagaccgc tgcccttcac cgcccctttt cgctggtggc ttccctgaaa
    59461 aaagggttcg aagatcatgg cgcgttcctc ttgcggaatg ggcgtgcctg tattgatgac
    59521 atcaatataa acccgcgcac catgtgaact gctgcgaagg caaatgttac cggattcagc
    59581 cccgtagtgc accgcattgg agtaaagatt atccagtacg ctcatcagca gcattggctc
    59641 cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc atcattttag cccgtgcggg
    59701 caggctatga gcagaaacca ctgtctccac cagcggtgct aattcaacat tctccagttc
    59761 caccgcactg tccgcctgtt tacggttgta atcaagcagt tgttcgatca gtttttgcaa
    59821 attgcggctg ctgctatcaa gaatgctcac cacctctttt tgctctggcg taagcggccc
    59881 gacaacctgg tcagccagta attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa
    59941 ttcatgagat aaatgtctta aaaattgatg gcgttgggat tccagccatg acaggcgctc
    60001 acttaaccaa agaatacgtt gcccaaccga gcgtaactcg ctcggtccac tgaacgagac
    60061 gctattgccc agagaacgcc cttcccccag acggttgatc atgcgctcga tatttttcac
    60121 cggaccaata atcatccgcg tgaaaagcag taccattacc agactcacca gaaatagcac
    60181 cagcgattgc caaccaaaat attgcccacg ttcggcgatt tcacgctgaa gttgctgccc
    60241 acgagagaac accactgtgc gcgtggcctg caccatttcg gtattggcac tggcaaaggc
    60301 ttcaagacgc gcggcggcgg cagcatcggg accactgttg ttacactgaa gttgggccag
    60361 attgttcaaa tcctgacgca atgcctggta aagtttatcg tctggcagta cgcctgcgtg
    60421 ggcatcgagc atttcgctgt aacgcttgcg ctggctttga taaaccttcg caagcgtagg
    60481 gtcgtccagc acgcaatact gacggtagct acgctccatc tccagcgccg cgttggtcat
    60541 tgcttcgctg cgccgggcat cgataagcgt ggtgcggttg accagcgccg cctggtcgct
    60601 aagcgcattc aggctttgcc aggcttgcca tgccagcacc aacaagggca gcagaatcag
    60661 caaaaatgcc agcattacca gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa
    60721 ggtgttactc tcgtcagacg cgaatagcct gatgctaacc gaggggaagt tcagatacaa
    60781 caaagccggg aattacccgg ctttgttatg gaataaggcg gtgcctaact cgacgtttcg
    60841 cccgatggtt gatatagcta cgctgatatc agaagttgga cggcaggcac cttgttgtgc
    60901 gtcattcgta ttttatgtag cacgtcccga aggggctgac ataagtcggt gaatgagcca
    60961 ctgcttacta ttatgcagta actgtgccaa taaagaaaat agtttggtaa cgtactgatt
    61021 atacgctgct ttgaggggtt tatggcttct ccgctgtttg aatgtaaatc agccactgtg
    61081 tcgctaaaaa gagacaactt aagataaact tattagatta cattaaaatc aatcatttaa
    61141 gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt gatttacaga cacaaaaaag
    61201 ctccctgagt tgggagctta taatagcggt tggtatttaa tgccggatgc ggcgtgaatg
    61261 ccgcatccgg gctgcaaaac caatgggctg acgacttacc ccaactgctt acgcgcattg
    61321 cggaaaatac gcatccatgg gccatcctcg ccccagtttt ccggatgcca ggagttgctg
    61381 acagtacgga aaacacgttc cgggtgcggc atcataatgg tgactcgacc actttcagtc
    61441 gtgactgccg taataccgtt cggagaaccg ttcgggttag ccgggtaggt ttcagtgact
    61501 ttgccgaagt tatcgacata gcgcagtgcc accagccctt tgctttccag tgccgccaga
    61561 tgcgccgcat cacgcacttc cacgcgccct tcaccgtgag agacagcaat cggcatttgc
    61621 gagcccacca tcccctgcaa cagcagcgac gggctttggg tcacttcaac caggctgaaa
    61681 cgcgcttcaa agcgatcgga ggtattgcgc acaaaacgtg gccacaactc actacctggg
    61741 atcagttcac gcagattaga catcatctgg caaccgttac atacccccag cgccagcgtt
    61801 tgcggacggt ggaagaaggt tgcaaactca tcgcgtacac ggtcattgaa caggattgac
    61861 ttcgcccaac cttcaccggc acccagcaca tcaccgtagg agaaaccacc gcacgcgacc
    61921 agggcgtgga aatcttccag gcccgtgcgt ccagccagca ggtcactcat atgcacgtcg
    61981 atagcatcaa agcctgcacg gtggaaagct gccgccattt caacatgcga gttcacgccc
    62041 tgctcacgca gtacggcaac tttcgggcgc gcgccagtgg caataaacgg tgctgccaca
    62101 tcttcgttga tatcgaacga cagttttaca ttcaggcccg gatcggcgtc gtttgatttc
    62161 gcctggtgct cctggtcagc acactccggg ttgtcacgca ggcgctgcat ctgccaggta
    62221 gtttctgccc accagacacg caacgtagtg cggctttcgc tgaatacagt ctgcccgttg
    62281 gcggtaatca caaaacggtc accggaaacc gcctgcccaa cataatggac gcaatcagca
    62341 agcccatgct gtgccagtac ggactcgacc gcttcacggt cagcggcacg aacctgaatc
    62401 accgcaccca gttcttcgtt aaacaacgcc gccaggcgat cgtcacccag agagccgata
    62461 tccgcatcaa tgccacaatg accagcaaag gccatttccg ccagtgtgac caacagaccg
    62521 ccatcagagc ggtcgtgata cgccagcagc ttacgctgtg caaccagcgc ctgaatcgcg
    62581 tcatagaagc ctttcagttg cgcgacatcg cgtacatctg ccggtttgtc gccaagctga
    62641 cgataaacct gcgccagcgc cgttgcgccc agcgcgttat tgcctttgcc caaatcaatc
    62701 agcagcagtg cgttatcttc ggtagaaagc tgcggcgtga tggtgtgacg tacatcttcc
    62761 acgcgggcaa atgcagaaat caccagcgac agcggcgacg tcatttcgcg ctcttcgtta
    62821 ccttcctgcc agcgggtttt catcgacata gagtctttac ccaccgggat cgtcaggccc
    62881 agcgccggac aaagctcttc gcccacggct ttaacggctt catacaggcc cgcatcttca
    62941 ccagggtggc ctgccgccgc catccagttg gcggaaagtt taatgcgttt gatatcgcca
    63001 atctgtgttg cggcaatatt ggttaacgct tcaccgaccg ccagacgggc agaggcggcg
    63061 aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca tcgcttcacc gtagtagctg
    63121 tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca cctgccacgg acccaccatc
    63181 tgatcgcgcg ctaccatgcc agttacgctg cggtcaccga tggtcaccag gaaagttttc
    63241 tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt ctgcaatggt gatcccttca
    63301 cggaccagcg catcgccctt cgctttcagc gtttgtacat cgcgggtcat cttcggcgtt
    63361 ttaccaagca ggacgtccag cggcagatcg atcggctgat tatcaaaatg acgatcgtgc
    63421 agagaaagat gcagttcttc ggtcgcttca ccaatcaccg cgtagggtgc gcgctcacgc
    63481 ttacacagtt cgtcaaacag cggtaattga tcggcagcaa ccgccagcac gtagcgttcc
    63541 tgggattcgt tacaccagat ttccagcggg ctcatgcctg gctcgtcgct cagaatgtcg
    63601 cgcagttcaa atttaccgcc acgcccgccg tcgctcacca gttccggcat agcgttagaa
    63661 agaccgcctg cgccaacgtc gtggataaac agtattgggt tggcatcacc aagctgccag
    63721 caacggtcaa tcacttcctg gcagcgacgc tccatctccg ggttgtcgcg ctgtacggaa
    63781 gcaaagtcga ggtcggcatc agactgacca gacgccatgg aagacgccgc gccgccgcca
    63841 agaccgatgt tcattgccgg gccgccgaga acgaccagct tcgcgccaac gttgatctcg
    63901 cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc ccgccagcat gatcggtttg
    63961 tgataaccgc gcagctcttc gccgttgtgg ctgttcactt tttcttcata agtacggaag
    64021 tagccgttca gtgccggacg accaaattcg ttgttaaacg ccgcaccgcc cagcgggcct
    64081 tcggtcatga tgtccagcgc ggtgacaatg cgctcaggct taccgaaatc ttcttcccac
    64141 ggctgttcga agccaggaat tcgcaggttg gaaacggaga aaccaaccaa acccgctttc
    64201 ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt caccgccgga accggtcgcc
    64261 gcgcccggcc acggagaaat cgccgtcggg tggttgtgag tttcgacttt catcagaata
    64321 tgcgccggtt cctgatggaa atcatagcgg cccgtttcgt ggtcagcaaa gtagcggccc
    64381 acttcagaac cttccattac ggcggcgtta tctttataag cagagagaac gtgatctggc
    64441 gtggtttcga aagtattttt gatcatcctg aacagcgatt tcggctgttg ttcaccgtcg
    64501 ataatccagt cggcgttaaa aattttgtgg cggcagtgct cagagttcgc ctgagcgaac
    64561 atatacagct cgatgtcgtt cgggttacga ccaagctttg tgaaagcatc ctgcagatag
    64621 tcaatttcat cttccgccag agccaggcca agacgcaggt tagcgtcgat cagcgcctga
    64681 cggccctgcc ccagcaaatc aacgcttgtg accggagtcg gttgatggtg ggcaaacaac
    64741 tgctctgcat catctaaatc aaaaaagacc gtttccatca tgcggtcgtg cagttcagcg
    64801 gtaacctgct gccattgttc attggtcagc gtaccggctt ctatatagta agcaacgccg
    64861 cgctcaaggc ggtttacctg ttgtagcccg cagttatggg caatatcggt cgctttcgaa
    64921 gaccagggag agatggtgcc aggacgcggg gtcaccagca ggagtttgcc ttgcggggcg
    64981 tggctggcga gtgccggacc atatttcagc aggcgttcaa gttgtgcgtg ctcatcatcg
    65041 tttaacggcg cattgaggtc agcaaaatgg acatactcgg cgtaaatatt gtgaaccggg
    65101 agcctggcag cctgaaaacg tgccagcagt ttgttgattc ggaatgccga cagtgcaggc
    65161 gaaccacgca gaatttccat cataagtctc tcgtcttcta agctttcggt gtacccaagg
    65221 gggggaaacg ggcgtcatta taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa
    65281 taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg gctgtaataa gttgccatct
    65341 ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa cctttcacta ttattacgtt
    65401 ttttcaagct gggacgcgca cgacacagag aattaactaa ttgaaaaaat taaagattaa
    65461 ttatctgttc atcggcattc tggcactgct gctcgtggtc gctctctggc catccattcc
    65521 ctggtttggt aaagccgaca accgtatcgc cgccattcaa gcgcggggac agttgcgtgt
    65581 aagcaccatt catactcccc tgacttataa cgaaatcaac gggaaacctt ttggcctgga
    65641 ttacgaactg gcgaaacagt ttgccgatta cctcggtgta aaactgaaag tgaccgtgcg
    65701 gcagaatatc agccagctgt ttgacgacct cgataatggt aacgccgacc tgctggcggc
    65761 aggacttgtc tataacagtg agcgggtaaa aaattatcag cctggcccta cctattattc
    65821 cgtgtcacaa caactggttt ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct
    65881 gacggcggag caactcaccg ttgcaccggg tcatgtggtg gttaacgatc tccagaccct
    65941 gaaagaaaca aaattcccgg aattaagctg gaaggtagac gacaaaaaag gctctgcgga
    66001 attaatggaa gatgtcatcg aaggaaaact cgattacacc attgctgatt ctgtcgccat
    66061 cagcctgttt cagcgcgttc acccggagct cgccgtagcg ctcgatatca ccgatgaaca
    66121 accggtgact tggtttagcc cgttagatgg cgataatacc ctttccgccg ccctgctcga
    66181 cttcttcaac gaaatgaatg aagacggtac gctggcacgc attgaagaga aatacctggg
    66241 gcatggcgat gattttgatt acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt
    66301 actgccgcag ttaaagcccc tgtttgagaa atacgccgaa gaaattgact ggcgtttgct
    66361 ggccgctatt gcttatcagg aatcgcactg ggatgcacag gccacttcac cgacgggtgt
    66421 gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac
    66481 cgatgccgaa cagagcatca gcggtggcgt gcgttatttg caggatatga tgagtaaagt
    66541 gccggaaagt gtgccggaga acgagcggat ctggtttgcc ctcgctgcgt acaatatggg
    66601 ctatgcgcat atgctggatg cccgcgccct gacggcaaaa accaaaggga atcctgacag
    66661 ttgggctgac gtaaaacagc gtctgccttt acttagccag aaaccctatt acagcaagct
    66721 gacttacggc tacgctcgtg gacatgaagc ctacgcttat gtcgaaaata ttcgtaagta
    66781 tcagattagc ctggtgggtt atctgcaaga gaaagagaag caggctacag aagcggcgat
    66841 gcaactggcg caggattatc cggcggtatc gcctacggag ttgggcaaag agaaatttcc
    66901 ttttctctcg tttctttccc agtcgtcatc aaactatttg acccattctc cctctctgct
    66961 gttttccagg aaagggagtg aagagaaaca aaattaatcc gtcgaggatt gcgctttttt
    67021 ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc
    67081 atccgccagt attccttccg taatttccac tcggtgattc atacccggat gatgcagcac
    67141 atccattaaa gatcccgcag cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc
    67201 aatgcgactg tggatcatcg ctccggcaca cattacacat ggttcaagcg tgacatacaa
    67261 cgtggcgtcg atcagacgat aattttgcat caccagacca ccctgccgca gggccatgat
    67321 ttctgcatgt gcggtgggat catggcgacc aatcgggcgg ttccagcctt cgccgattac
    67381 ccgattgtta tgcactaata ccgcgccgac cggcacttcc cgctcatccc aggcacgttt
    67441 cgccagcgtc agcgcgtgac gcatccagta ttcgtggcta aattcgactt cagacaaaaa
    67501 gaaaactccg gttataaaag cgcggcgcat tatacacgga cgctatgctt tactctattc
    67561 cagttgctgg agttcaccgc gcggggttac tcgccaacga tgctgacaga aataaagcag
    67621 cggattgtcc tgattactgt cgctatagcc actgtacagc cgcagcggag tgccgatttt
    67681 gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc atcgtcaata cccaaccacc
    67741 atagccacgc tgaatttggc tggcgataag attaacccgc ggcagccagg gcgtatcgaa
    67801 ataaaccgct tcaaccagcg gctgcggaga gccggtaatc aaccagatat cagcatcgga
    67861 acttaacagg taggtggtta atcgctcctg aaccagcgga aaggcggtaa cattgtcgcg
    67921 aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt gcttcgctgt gaccaaaagt
    67981 gcacccccac agaagcagac tcatcggcca gcgtgccgca cgacctttta tcaataacgc
    68041 aatggctata atcggtaaca acggcaggac aagtaacgca ttcagcggtt ggcgacgcag
    68101 taaatagcgc agaaaactgc cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa
    68161 aaacaccaca cgacgctcgt gagttgccaa accatactcc tctggactga aacatcctga
    68221 ttaattttcg ctactacata gcctaacaga tagatcatca cttttccggc aacaatcgac
    68281 cgcatcatgg ctaactggaa tttttaattc atggcaatta gcggcaatgg aatataaaat
    68341 tcactcgcgt gtctctcata ttgcctgttg tcgccactat ttcatcgcga ggaaagatat
    68401 gaacggctta cttcgtatcc gtcagcgtta ccaggggctt gcccagagtg ataaaaaact
    68461 ggcggattat ctgctgctac aacctgatac ggcgcgccat ttaagctctc agcaactggc
    68521 caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa
    68581 aggttttccg gcgcttaagt tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc
    68641 ctccgtgccc attcataacc aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa
    68701 actgattaaa gaaaatactg ccgctatgta cgcaacgcta aacgttaata gtgaagagaa
    68761 actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg ataattctga ccggtattgg
    68821 cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg atgaagattg gcttcaatgc
    68881 tgccgcagtg cgcgatatgc atgcgctact cgcaacagta caggcgtcgt cccctgacga
    68941 tctgttatta gccatttcct acaccggtgt acgacgcgag ttaaacctgg cggcagatga
    69001 gatgctgcga gtgggcggaa aagtgctggc gattaccggc tttactccga atgccctgca
    69061 acagcgtgct tctcattgcc tgtataccat tgccgaagaa caagcgacaa acagtgcttc
    69121 aatctctgct tgtcacgctc agggaatgtt aacggatttg ctgttcattg cgctgattca
    69181 gcaggatctg gaactggcac cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact
    69241 ggtctgagta aagaatgcgc gtataatgcc cgcccggttt gtgttgtttt gagagtttcc
    69301 ttatggcgtt gttaatcact aaaaaatgca tcaattgtga tatgtgtgaa cccgaatgcc
    69361 cgaatgaggc gatttcaatg ggagatcata tctacgagat taacagcgat aagtgtaccg
    69421 aatgcgtagg gcactacgag acaccaacct gccagaaggt gtgcccgatc cccaatacta
    69481 ttgtgaaaga tccggcgcat gtcgagacag aagaacagtt gtgggataaa tttgtgctga
    69541 tgcaccacgc ggataaaatt taatattcta ctctggaagt agagtattaa ttatattact
    69601 gggaagcctt aacgccatta tatttattta attgatgaca ttagcataat cattcactaa
    69661 gttaatttat atagtatctg ccaagacact tatttatagt tattaaaggc gcgtccgatt
    69721 ggttcaccgg acgcacctta agtacgtttc cttgtgttat aagaacagaa ggatcagctg
    69781 taaaacagca atgatgattt tgatgacccg tttaatcagg tatcggcaat cagtcattcg
    69841 tttttcctta aacaaggaat gcagctgttc agattagccc ttacatctcc ccaaaactga
    69901 acgtgcgagt tattgagggt gcatgctgca ctccacacca gagctttgac gacaccactc
    69961 gtttcaatgg gggaattctg tggcatggtg taaagcacag caaaatcttc aataacgaag
    70021 ccaattttaa tgtacttacg aattggcggt cacgttaatc tttccatcaa tattgctttc
    70081 ttcgtaaagg ctcgagtttt tatgctaaag attgcaagtt gcttgtaaaa gataagtaca
    70141 ctgatccata atcgctgttg ttgagggtgc atgctgcaca aaattaaagt taaaaagtaa
    70201 aacccccgtt ccttaccagt tcgggggttt tactttttaa agagaacggt attattttaa
    70261 ctttcaataa ttaccgtggc acaagcatag tggcgttcat ctgccagcgt tacatgcata
    70321 tttacaacgc cgagcttttc cgccagtttt aatgcctcac cccatagccg tagccgtggt
    70381 ttgccgagct catcattgaa tacttcaaat tgattaaacg ccagaccatt gcggatcccg
    70441 gtgccaaacg cttttgctgc ggcttctttc acagcaaaac gcttcgccag aaaacgcacc
    70501 ggctggtggt gcgttttcca gattgcccat tcgttatcgc ttaatacgcg gcgtgccagg
    70561 cgctcaccgg atcgggcgat cactgcttct atgcgagcga tctctacgat atccgtgcct
    70621 aaacctaata ttgccattag ccacgcgctt ccagcatcag acgcttcatt tctgccaccg
    70681 catctttcag tccggtcatc actgcacgac caataatggc atgaccgata ttcagttcat
    70741 gcatctcagg gatggcggca atagctttca cgttgtgata ggtcagaccg tgaccagcgt
    70801 taactttcag accgagacta gcggcaaagg tcgcggcttt ggcgatacgc gccagctctt
    70861 gcgcctgttc ggcgtcagtt ttggcatcag catagcaacc ggtgtggatc tcgataaacg
    70921 gtgcgccaac ctctgccgca gctttgatct gctcttcatc ggcgtcgata aagagagaaa
    70981 cctggatccc ggcatctgcc agacgtttgc aggcatcgcg cattttgtca cgctgccctg
    71041 cgacgtccag gccgccttcg gttgttactt cctgacgctt ttccggtacc agacagcaaa
    71101 aatgtggctt cgtctcaacg gcgatcgcca gcatctcttc ggtcaccgcc atctccagat
    71161 tcatgcgggt atccagcgtc tgacgcagga tgcgcacatc acggtcagta atgtggcggc
    71221 gatcttcacg taaatgcacg gtaatgccgt ccgctcccgc ctgctcagca ataaacgccg
    71281 cctgcaccgg atccgggtaa gcagtaccgc gtgcgttacg cagcgttgca atatggtcaa
    71341 tgttgacgcc taacagtaat tcagccatga caatcctcat cattcataat gtgttttcac
    71401 cgttcgctta ggcataaact gccggaacag ttccctgctc tttaatggtt taccgccaag
    71461 atacggctta agcgccatgc gggtaaagcg tttcgcggcg cgtagtgtgt ctgcgtcagg
    71521 aaattcccgc gcgtttaacg cttttaactg ccttccggtg aacgttttat tgtcgataac
    71581 gacgcttgcg ataaaccctt tttcttcgcg ataacgatac gtcatggtgt catctaccgg
    71641 ctcgccgcta cccgcacaat gggtaaaatt gacgccatag cccagatgcc cgagcagtgc
    71701 caattcaaag cggcgcagcg cgggttctgg cgtaccagtg gcacctgcaa gagactgaat
    71761 gcagtgcaag taatcgaaaa agagttcaga gaagcgcgtc tcgtattcca gtacgcggga
    71821 gagaagttcg ttgatgtaca gaccgctgta aagcgtgata ccgcttaatg gcagcgccag
    71881 cgagacagct tcagcactgc gcagcgtttt gacttcgcca cgcccgccga aacgtagcaa
    71941 gagaggggtg aaaggctgta atgcaccttt cagagtagag cgtttagagc gtgcgccttt
    72001 ggcaaccaga cgcacgcgac ccgattcctc cgtgaagacg tccagcatca ggctggtttc
    72061 gctccacggg cgactatgca ggacaaatgc gcgctgccag ccttccatcg gagtgactct
    72121 taaagatcgt caacgtaacc gagactgcgc agtgcgcgtt cgtcgtcggc ccaaccggat
    72181 ttcactttta cccacagttc aaggtggaca ggcgcttcga acatttcctg catgtcttta
    72241 cgcgcttcaa tcccgatggt tttgatcttg gcccctttgt tgccaataac catcttcttc
    72301 tgcccttcac gctcaacgag aatcaaacca ttgatgtcat aaccaccgcg ttcgttagag
    72361 acgaaacgtt cgatctccac ggtcacggag tacggcagtt cagcgccgag gaaacgcatc
    72421 agtttttcgc ggatgatttc agacgccata aaacgctgtg agcgatcggt gatgtaatct
    72481 tccgggaagt gatgggtcgc ttcagggagg tgcttacgca cgattgctgc gatagtgtcg
    72541 acattcagcc cggtttcagc agagatcggc acgatatcga ggaagttcat ctgacttgcc
    72601 aggaactgta agtgcggcag cagatcggct ttttcctgca cgttatccac tttgttcacc
    72661 gcgaggatca ccggcgcttt gccatcgcgc agtttgttga gcaccatttc gtcgtccggc
    72721 gtccagcggg tgccttcaac gacaaaaatc accagctcaa cgtcgccaat agagctgctc
    72781 gccgctttgt tcatcaggcg gttaatagcg cgtttttctt ccatatgcag gccaggggta
    72841 tcaacgtaga tcgcctgata cgcgccttca gtatggatcc ccacaatgcg gtgacgagtt
    72901 gtttgcgctt tgcgggaggt gatggagatc ttctgcccca gcagtttgtt caacaatgtg
    72961 gatttgccaa cgttcggacg tccgacgatg gcaataaatc cgcagtaact tttatcgatg
    73021 ctcattccag ctccagtttt ttcaacgcct gttcggcggc agcctgctca gccttacgac
    73081 ggcttgaacc tgtgccaacc accggttcac tcaggccgct gacctggcag tggatagtaa
    73141 attcctgatc gtgcgcttcg ccacgtacct ggactaccag ataagtcggc agcggcagat
    73201 ggcgaccctg caaatattct tgcaagcgcg ttttcggatc tttttgttta tcgcctgggc
    73261 taatttcgtc caaacgagtt tgataccagt tgaggattaa tttctcgacg gtttgaatat
    73321 cactgtcgag gaatacgcca ccaattaatg cttcgacggt gtcggcgaga attgactcac
    73381 gacgaaatcc accgctttta agttcacctg gccctaaacg taagcactcg cctaactcaa
    73441 attcgcgcgc cagttccgcc agcgtattgc cacggaccag cgtggcgcgc atccggctca
    73501 tatcgccttc atccacacga gggaaacggt gataaagcgc attggcgata acgtagctca
    73561 gaatagagtc gcctaaaaat tctaaacgct cgttatgttt actgctggca ctacgatgag
    73621 ttaatgcctg ctgcaacagt tcctgatgat taaaagtgta gcccagcttc cgttgaagcc
    73681 gattaattac gatggggttc atgcgatacc aataaataaa tgcgtcaaca attcagcaca
    73741 cgaaacagac ctgatataca tggctctgct aactgcttcg ctgcagtttc agtttaccgg
    73801 tatatgggga ccaacgctgt ttcgtgtgcc gtggcaacct ggaggtgcca accttaaact
    73861 tcgggggaat attctataca caacgacggg ggatgtcgtt agccacggga gatttatctc
    73921 ataaataatt cacgttgtcg ccataacggc gacaacgtga acgaagatga gctattaatg
    73981 gatgccgcca atgcgactta agcgcacacc agtcggccat tcgccttctt gcttatcgaa
    74041 gctcatccag atagccgttg cccgaccgac cagattcgct tcaggcacaa acccccaata
    74101 acggctgtcc gcgctgttgt cgcggttgtc gcccatcatg aagtattgtc ccggaggaac
    74161 aatccaggtt gccagttgtt gccctggctg ctggtaatac atccccacct gatcctgcgc
    74221 aatcggcact gtcagaattc ggtgcgtcac atcacccaat gtctctttac gctcggaaag
    74281 acgaattcca ttttctttgg tttcgttttt cggcacttca aagaatccgc tggtcgcttc
    74341 cccaccatta cggcgtgaga aggtctgaac gaaatcgctc ggttccacgt ttgagtaggt
    74401 gaccggcagc gcgttttcac acgcctggcc ggaactgcat cccggttgaa tcgtcagctc
    74461 tttcgagacc ggatcgtaag tgactttatc gcccggtaaa cccaccgcgc gcttgatgta
    74521 atcaagcttt ggatcttccg gatatttaaa gaccacgata tctccgcgtt tcggatgacc
    74581 ggtttcgatc agcgttttct ggtagatagg atctttaatg ccataagcaa acttttctac
    74641 cagaataaaa tcaccgatta acagagtcgg catcatcgaa cctgacggga tctggaacgg
    74701 ttcataaata aacgaacgca caatcaatac gatagccagt accggaaaaa cagaagcacc
    74761 ggtttccagc cagccaggct tcggcgcaac ctttttcaac gttgctttat ccagtgagtc
    74821 accggcagcc gcctgcgccg ctgcctgacg ttcccgccgt ttaggtgcga aaaagaattt
    74881 atccacgcac cataaaatgc ccgtcaccag tgtggcaatc accagaatca gggcaaacat
    74941 attcgccatg ccaactccta agggttattt gttgtctttg ccgacgtgca gaatggcgag
    75001 gaacgcttcc tgcggcagct cgacgttacc gatctgcttc atgcgtttct taccttcttt
    75061 ctgcttctgc agcagctttt tcttacggct gatatcgccg ccataacatt tagccagtac
    75121 gtttttacgc agctgtttca cggtggatcg cgcaataatg tgcgtaccaa tcgctgcctg
    75181 aatggcgata tcaaactgct ggcgtgggat cagatctttc atcttctcca ccaactcgcg
    75241 accgcggttt tgcgaattat cacggtgggt gatcagcgcc aacgcatcaa cacgttcacc
    75301 gttgattaat acgtctacac gtaccatgtc ggacgcctgg aagcgtttga agttgtaatc
    75361 cagagacgca taaccacgcg aggtagattt caggcgatcg aagaagtcga gcaccacttc
    75421 cgccatcggg atctcgtacg tcagcgccac ctgattaccg tggtaaacca tattggtctg
    75481 cacgccgcgt ttttctacgc acagcgtaat aacgttgccg agatatgcct gcggcagcag
    75541 catgtgacat tcggcaatcg gctcgcgcag ttcgtagatg ttatttaccg ctggcagctt
    75601 ggatggactg tcgacgtaaa taacttctct tgacgtggtt tcaacttcat acactacggt
    75661 cggcgcagtg gtgatcagat ccagatcgta ttcacgttcc agacgttcct ggatgatctc
    75721 catgtgcagc aggccgagga agccgcagcg gaaaccaaag cccagcgcgc tggagctttc
    75781 cggctcatag aacagtgaag catcgttcag gctgagttta cccagcgcgt cacggaaggc
    75841 ttcatagtcg tcggaactta ccgggaacag accggcgtat acctgcggtt tgactttctt
    75901 aaagccaggc agcgcctttt ctgccggatt acgcgccagc gttaaggtat cgccgactgg
    75961 agcgccgtgg atatctttaa tcgcacatac cagccagcct acttcgccac atttcagttc
    76021 agtgcggtca acctgtttcg gcgtgaagat gcccagacgg tcggcgttat aggtctgccc
    76081 ggtactcatg actttcactt tgtcgccctt acgcagggtg ccgtttttaa tacggataag
    76141 tgaaacaacg cccaggtagt tgtcgaacca tgagtcgata attagtgcct gcaacgggcc
    76201 ttccggatcg ccttccggcg gcggaatgtc gcgcaccaga cgttcgagaa cgtcctgcac
    76261 gccaacgccg gttttcgctg aacagcgcac cgcgtcagtg gcgtcgatgc cgacgatatc
    76321 ttcaatttct tccgccacgc gttcaggatc ggctgccggc aggtcaatct tgttcagtac
    76381 tggcaccact tcgagatcca tttccatggc ggtgtagcag tttgccaggg tttgcgcttc
    76441 cacgccctgc ccggcgtcga ccaccagcaa tgcaccttca caggcagcca gcgaacggga
    76501 aacttcatag gagaagtcta cgtggcccgg ggtgtcgata aagttaagct gataggtttc
    76561 gccgtcagac gctttgtagt ccagcgtcac gctttgcgct ttgatggtaa tgccacgctc
    76621 acgctcaaga tccatggaat cgagaacctg cgcctccatt tcacggtcag acaggccacc
    76681 gcagatctgg ataatacggt cagacagcgt cgatttaccg tggtcaatgt gagctatgat
    76741 cgaaaagtta cgtatattct tcataaagta tgattattgt gccttaatgc ccggttaacc
    76801 aggcttttag aagtcgctgt tctgagctta acgtctgtat taatagaaac gccgcattct
    76861 acactacaac attgaggcga ggaaatgttc ataccgtatg gattgtggta tctggaaacg
    76921 tcctcgcatt tgttatgcaa aatgcaacaa agccagtgaa atcactggct cgcgtcttcc
    76981 gaagatgttt caaatcgcac aaggccaggc ggcaatgcca cgcttaagat gatcggttgc
    77041 cattctgccc gggcagcaaa cttacgcgag tagccgcgcg caatcaggaa cccgccaata
    77101 ccaccgagga tcgcaccaca taatgctgcg acgtcagaag caaagagtag ctgaaatagc
    77161 gaagcgataa ggaataatcc caccagcggc gacatataaa ccagtaatgc ggagctaagc
    77221 aggctgcctt cggcgatccc caattccact ttttgccccg gcactaacgg ttcatcacag
    77281 ggtacgacaa tggtatgcgt ggtttgcggg ccaagtttat tcaacacgcg gctaccgcaa
    77341 ccggcgcgtg aagcgcagct gctgcatgaa gctttaacat cacaactgac cagcgcctgc
    77401 ccgttttgcc aggagacgac ggtagcccac tctttgatca ttgcgctgcc ccgaacttaa
    77461 tattctcggc aatgcgtttc gccgtttgcg gcggcagttc accgacaatg gtgatttcgg
    77521 cgttatcacg tacgcttgta ctgacggttc tgcgtccggt gcgcaacatc tgatcggtgc
    77581 tcgatggcgt agcgcggtta acgtttaccg agaagctgaa taatccgtca gaatagagac
    77641 gtgattcgat aggcatgttg tccatcgtcg gtagcggacg tcgactactg gaaacttcgc
    77701 taaaaccctg tggcaaccag gttggcgtcc agctgaattt agctttttca cctacgggaa
    77761 cagaaagcaa cggcggcaaa tttaccttcg ccagcgtctg catactgctg ctgatatcct
    77821 gattgacgtt aaaagcaatc acgcgaaatt gttccagcgt ttcaccatcg cgatcaagga
    77881 gatcaacccg catcggtaat ttcgattcgg tgtccatcca cacgatgtag ctgtagcgtg
    77941 taccatctcg ggcaaccacg cgaatgactt cgcaaagacg atcagcaata cgcgtgcgcc
    78001 cgacggagat aaagtcgtag taaggagaaa ggcgtttgaa atcggtatag ataagcgatg
    78061 gcagagaatc aacaatgtaa tcgccattaa gcgtgaacgg ttcaagtccc ggttcaaaat
    78121 agctgatttc attgccgcgc tgtaccactt cccggcgcgg gccatccatt tgcaacaatt
    78181 gtgcaagagg acggttatcg aggcgtgcat gtcgataacg cagagactca acaccctgtt
    78241 tattgatgct gatgaatgac agctcgtaat tcagtgactg actggccagg ttcatctgct
    78301 gtaataacgc cccggacgcg ggagtggccg aggcgttagc agagaataac aggctacctg
    78361 tcactaatga catggcaaac caaagttgct tcattactgc gattgcgttc ctaaagtttg
    78421 aattcctggc acctgtacag cggcttgctg ggtttgcgcc tgctcaaact gaagctgttc
    78481 agagtggagt cggcgttgca gttcgtaatc ctgcaacatt gcattaatgc gacgacgctg
    78541 ctcctgtacc tgctgctgtt gaccattgtt tgcggtcgct tcagaaggta ctcccaggct
    78601 taccgggctg gctttaccca tcatcggcag tgtattaaat accggcgttt cgggctgctg
    78661 ggacgtttca gattgtccat tatagtgctg gacgccaacg ataactgcaa gcgatacgca
    78721 tgctgctacg cccatttggg taagctgtgc cgcccacgga cgtactttct gccagaatgg
    78781 cattttctgc cattgatgcg gcgcaggctg ggcttccggg atcaatgtcg ccggttgacg
    78841 tactggctct tcttcaatgg cggccatcac gcgtgaagag atatcgaaat ggagcacctc
    78901 gggagtatca ccccgcattg aatcacggat taagtgatag ctttcccagg ttttctgcat
    78961 ttctgggtta tgagccagtt cgttaagcag ctcactatcc agcgtttcgc catccattaa
    79021 agcggaaagt tgttctttct gcatgcctaa tacccttatc cagtatcccg ctatcgtcaa
    79081 cgcctgataa gcggttgaac tttgttatca atagcttccc tcgctcggaa gatacgtgaa
    79141 cgcaccgtac ctaccggaca atccatgata gcggctatct cttcatagct caggccatcc
    79201 agctcccgca aggttattgc catgcgcaaa tcttccggga gggactcaat agttcggaaa
    79261 actatctgtc tcagttcttc tgacaacatt aagttctcag ggttcgaaat ttctttcaac
    79321 gcgccgccac tttcgaagtt ttcagcttca atggcatcca catcactgga aggtggacga
    79381 cgcccctgag caaccaggta atttttcgct gtatttacag caatccgata cagccatgta
    79441 taaaaagcgc tatctccccg gaacgaatcc agcgcacgat aggctttaat aaaagcttct
    79501 tgtaccacat cgggaacatc acccgacggc acatagcggg aaaccagact cgccacttta
    79561 tgctgatagc gcactaccag taagttaaag gctttctgat ctcccttctg gacccgttca
    79621 accaggacct ggtccgttaa ctgctcgctc atccgaggta aagtctcccc aaaccaaatt
    79681 tccacgcgct atcgaaacgc cactccatta gctgcaattt gagcaagcaa agggttagag
    79741 tgtctcgttt ttgtaaagtt ccgtaacgca tctgtttttg tttgtcatgc tgtagacgga
    79801 tcattatcta tcattataag tctacagaat ctgaacatcg cattatctgt gtagaaatgc
    79861 ccatttaact gcctgaagag taacccaacg gcctttttat ttcaccacct aatcctccac
    79921 cagccagtaa cttctctttt tctcgccgcc ctgcatcagc gtgtttagca actgtaacaa
    79981 atattaaaat agcaggtgtt tattcgcaca acatgatgct atgctgacca aaccatgttt
    80041 agtaaattaa acaaagaaaa tgaatactct ccctgaacat tcatgtgacg tgttgattat
    80101 tggtagcggc gcagccggac tttcactggc gctacgcctg gctgaccagc atcaggtcat
    80161 cgttctaagt aaaggcccgg taacggaagg ttcaacattt tatgcccagg gcggtattgc
    80221 cgccgtgttt gatgaaactg acagcattga ctcgcatgtg gaagacacat tgattgccgg
    80281 ggctggtatt tgcgatcgcc atgcagttga atttgtcgcc agcaatgcac gatcctgtgt
    80341 gcaatggtta attgaccagg gggttttgtt tgatacccac gttcaaccga atggcgaaga
    80401 aagctaccat ctgacccgtg aaggtggaca tagtcaccgt cgtattcttc atgccgccga
    80461 cgccaccggt agagaagtac aatccacgct ggtgagcaag gcgcagaacc atccgaatat
    80521 tcgtgtgctg gagcgcagca acgcggttga tctgattgtc tctgacaaaa ttggcctgcc
    80581 gggcacgcga cgggttgttg gcgcgtgggt atggaaccga aataaagaaa cggtggaaac
    80641 ctgccacgca aaagcagtgg tactggcaac cggcggtgcg tcgaaggttt atcagtacac
    80701 caccaatccg gatatttctt ctggcgatgg cattgctatg gcgtggcgcg cgggctgtcg
    80761 ggttgccaat ctcgaattta atcagttcca ccctaccgcg ctgtatcacc cacaggcacg
    80821 caatttcctg ttaacggaag cactgcgcgg cgaaggcgct tatctcaagc gcccggatgg
    80881 cacgcgtttt atgcccgatt ttgatgagcg cggcgaactg gccccgcgcg atattgtcgc
    80941 ccgcgccatt gaccatgaaa tgaaacgcct cggcgcagat tgtatgttcc tcgacatcag
    81001 ccataagccc gccgatttta ttcgccagca tttcccgatg atttatgaaa aattgctcgg
    81061 gctggggatt gatctcacac aagaaccggt accgattgtg cctgctgcac attatacctg
    81121 cggtggtgta atggttgatg atcatgggcg tacggacgtc gagggcttgt atgccattgg
    81181 cgaggtgagt tataccggct tacacggcgc taaccgcatg gcctcgaatt cattgctgga
    81241 gtgtctggtc tatggctggt cggcggcaga agatatcacc agacgtatgc ctgatgccca
    81301 cggcgtcagt acgttaccgc cgtgggatga aagccgcgtt gagaaccctg acgaacgggt
    81361 agtaattcag cataactggc acgagctacg gctgtttatg tgggattacg ttggcattgt
    81421 gcgcacaacg aagcgcctgg aacgcgccct gcggcggata accatgctcc aacaagaaat
    81481 agacgaatat tacgcccatt tccgcgtctc aaataatttg ctggagctgc gtaatctggt
    81541 acaggttgcc gagttgattg ttcgctgcgc gatgatgcgc aaagaaagcc gggggctgca
    81601 ttttacgctg gattacccgg aactgctcac gcattccggt ccatcgatcc tttcccccgg
    81661 caatcattac ataaacagat aaaaagcctg ggtcagcgcc gtatacgctt cggaatagtt
    81721 ctggtctggc ccacgaatga ctaagcgatc gctaaagcat tctcccgcct gcggggagaa
    81781 tgccagcagc acccgatgcg gcagtcgcgc ttcgttttcc gccacatccg tccgcaaacg
    81841 taaatgccag cccatgctta atgccagttc agtaaaacca ttaccaatct gctctggcag
    81901 caccacgcag aaaaatccct cttcggtaat gcactccgcc gcacaggtca gcagcgatgg
    81961 gtgatcaagc gtagtggtat agcgagcctg ttcccgttga ggtgtcgcgc actctactcc
    82021 ctgctgatag taaggtgggt tactgatgat taaatcgaag cgaactgtct gctgtgtgat
    82081 ccactgctga atatccgccg tatggacgtt aatccgctct acccacgggg actggttgat
    82141 attttcctgc gcctgcgcgg cagcttcact ttccagctca acggcatcaa ttatcacgct
    82201 gccactggtt cgctgcgcca gcatcagtgc cagcaacccg ctacccgcgc cgatatcaag
    82261 gcaacgtttc accccagcca ccggtgccca tgcgcccagc aaaatgccat ccgttcccac
    82321 tttcatcgca cagcgatcgt gagcaacaaa aaactgttta aaagtaaatc cattacgacg
    82381 aagcacggat gtagactgtg acatgaaaat aaaaccttgc aggaaaaacg gcgatagcac
    82441 cgggtgagaa caatacccga gaagcgatat ccatacaaac agatgaagat tgcagccgta
    82501 acgtctataa tcagcgcccc acacagaggt agaacatgac tgtaacgact ttttccgaac
    82561 ttgaactcga cgaaagcctg ctggaagccc tccaggataa aggtttcact cgcccgaccg
    82621 ccattcaggc tgccgccatt ccgcctgcgc tcgatggccg tgatgtactc ggttctgcgc
    82681 cgacaggcac cggtaaaacg gcggcgtatc tgctgccagc gttgcagcac ctgctcgatt
    82741 tcccacgtaa gaaatccggt ccaccgcgta ttttgatcct caccccaact cgcgagctgg
    82801 cgatgcaggt ggccgatcat gcccgcgaac tggcgaaaca tacgcatctg gatatcgcca
    82861 ccatcaccgg cggcgtagcc tatatgaacc acgcggaagt gttcagcgaa aatcaggaca
    82921 tcgtggtcgc cacgaccgga cgtctgctgc aatacataaa agaagagaac ttcgattgcc
    82981 gcgcggttga aacgctgatc ctcgacgaag cagaccgtat gctggatatg ggcttcgctc
    83041 aggatatcga acatattgct ggcgaaacgc gctggcgtaa acagaccctg ctcttttcgg
    83101 caacgctgga aggcgatgcg attcaggact ttgccgagcg tctgctggaa gatccggtgg
    83161 aggtttctgc caatccgtcc acccgtgagc gcaaaaaaat tcatcagtgg tattaccgcg
    83221 ccgatgacct ggaacataaa accgcattgc tggtgcatct gttaaaacag ccggaagcga
    83281 cccgctcaat tgtgtttgtg cgtaagcgtg aacgtgtgca tgagctggca aactggctgc
    83341 gcgaagcggg catcaacaac tgctatctcg aaggtgagat ggtacaaggc aagcgtaacg
    83401 aagcgatcaa gcgtttgacc gaaggtcgcg taaacgtact ggttgcaacc gatgttgccg
    83461 cgcgcggtat cgacattcct gacgtcagcc acgtctttaa cttcgatatg ccgcgcagtg
    83521 gcgatactta tttgcaccgt atcggacgta ccgcgcgcgc cggtcgtaaa ggcaccgcaa
    83581 tttcgctggt ggaagcccat gaccatttgc tgctgggcaa ggttggccgt tacattgaag
    83641 agccgattaa agctcgcgtt attgatgagt tacgcccgaa aacgcgtgcg ccaagcgaga
    83701 agcagaccgg caagccatcg aagaaagtac tggcaaaacg cgccgagaag aaaaaagcta
    83761 aagagaaaga gaaaccgcgg gtgaaaaaac gccatcgcga caccaaaaat attggtaagc
    83821 gccgtaaacc aagcggaacg ggcgtgccac cgcaaacgac agaagagtaa tctcaatgcc
    83881 gggtttaagc ctggtattaa aagtgctggc aaacgcaaaa ctgcctgatg cgctacgctt
    83941 atcaggccta cgtggctcat gcaatatatt gaatttgcac gatcttgtag gccggataag
    84001 gcgttcacgc cgcatccggc atgaataaag cgcactttgt cgacaagtta aatgccgggt
    84061 ttaatcccga cattattttt atccagccac aaaactctct tcaacacact gaataaaaat
    84121 gtgcatcgcc gggctaaccg cttttccagc atgatgtgcg cacattgccg taatggtctg
    84181 cgactgttcg ccaaaaggca attcaattaa ttctccgctc tcaagctctt tttccaccgc
    84241 aaagcggggc aagtaactga caccgatatt cgccgcgaca caacgtttga tactttcaat
    84301 gcttaacagc tcaatggtgt tttccaccgt gctccgccgc tggcgcaatg tactttcaaa
    84361 gatctgccga aagacacatt gcggttcgtt gataataaag ctacaggcgt tatgtcttcc
    84421 cggctcagta aaatcgacat ctgcaatttg cggtgaagcc accagcacca gcgattgttc
    84481 acccaactct cgtcggttca gggcatcatc attccctacg cgataaaaga cgccgacatc
    84541 ggcctcatca ttcagtagcg catcacggat cacgtaacag ttcagcgact gcaacgataa
    84601 acgcactttt ggtgcgcgtt gccgaaaacg ctgcaacacc tgcggcattc ggtaagagag
    84661 caacgtttcg cccgaaacaa cgcgtagttc cccgtccgga tctgactcct ttttggcagc
    84721 ctcacgaagc gtatccatca ctcgggtaag ttcataaatg tgcggcagca acttttttcc
    84781 ttcgcgggta aggcacatcc gtcggccaat tttctcaaat aactggactg aaaactcctg
    84841 ctcaagctgc tgaatatgaa aagtcaccgt cgattgtgta cagcacaatt tttgcgaagc
    84901 tcgcaaaaag gaaccctctt ccaccacggt tttaagcgta ataaaacggc gcagatccat
    84961 aaccccaaac ctatcgaaaa tatcgaatct agaatataaa aacattcatt tttttaaatg
    85021 ttccgtgtcg ggtactgtct accaaaacag aggagataac aagtgacacc gaccctttta
    85081 agtgcttttt ggacttacac cctgattacc gctatgacgc caggaccgaa caatattctc
    85141 gcccttagct ctgctacgtc gcatggattt cgtcaaagta cccgcgtgct ggcagggatg
    85201 agtctgggat ttttgattgt gatgttactg tgtgcgggca tttcattttc actggcagtg
    85261 attgacccgg cagcggtaca ccttttgagt tgggcggggg cggcatatat tgtctggctg
    85321 gcgtggaaaa tcgccaccag cccaacaaag gaagacggac ttcaggcaaa accaatcagc
    85381 ttttgggcca gctttgcttt gcagtttgtg aacgtcaaaa tcattttgta cggtgttacg
    85441 gcactgtcga cgtttgttct gccgcaaaca caggcgctaa gctgggtagt tggcgtcagc
    85501 gttttgctgg cgatgattgg gacgtttggc aatgtgtgct gggcgctggc ggggcatctg
    85561 tttcagcgat tgtttcgcca gtatggtcgc cagttaaata tcgtgcttgc cctgttgctg
    85621 gtctattgcg cggtacgcat tttctattaa cgaaaaaaag cggaagaggt cgccctcttc
    85681 cgcttagtaa cttgctactt aagccttaca ggctttcagt aaaggtacga gcgataacgt
    85741 cgcgctgctg ttccggagtc agagagttaa agcgaactgc ataaccggat acacggatgg
    85801 tcagctgcgg atatttttcc ggatgcttaa ctgcatcttc cagagtttcg cgacgcagaa
    85861 cgttaacgtt caggtgttga ccaccttcaa cgcgaacttc tggtttcact tctactggaa
    85921 cttcacggta ttcaatgtca cccagtttgc ttactgcaac cacttcatct tctgcataac
    85981 ctgcttttgc aacgatgcaa cgcgcttcgc ctttttcgct gtccagcagc cagaaagagt
    86041 tcagcagatc gtcgttagcg gctttagtaa tctggatacc tgtaatcatg tgatgcctcc
    86101 ccggcaaaat tatttgattt gttcagcctg tcgcggccaa ttggtaaaac cattgttgct
    86161 tgagtgtata tatactcctc aaacaccctt gaatctttga tttaaatcaa taaaaaccac
    86221 acatcaagta ttgtcgcaaa tggattttat tgttttacat caacttatgc gggtgtgaaa
    86281 ttttaccaat ttacattttt ttgcactcgt ttaagtctaa aaaatgagca tgattttgtt
    86341 ctgtagaaag aagcagttaa gctaggcgga ttgaagattc gcaggagagc gagatggcta
    86401 acgaattaac ctggcatgac gtgctggctg aagagaagca gcaaccctat tttcttaata
    86461 cccttcagac cgtcgccagc gagcggcagt ccggcgtcac tatctatcca ccacaaaaag
    86521 atgtctttaa cgcgttccgc tttaccgagt tgggtgacgt taaagtggtg attctcggcc
    86581 aggatcctta tcacggaccg ggacaggcgc atggtctggc attttccgtt cgtcccggca
    86641 ttgccattcc tccgtcatta ttgaatatgt ataaagagct ggaaaatact attccgggct
    86701 tcacccgccc taatcatggc tatctcgaaa gctgggcgcg tcagggcgtt ctgctgctca
    86761 atactgtgtt gacggttcgc gcaggtcagg cgcattccca cgccagcctc ggttgggaga
    86821 cgtttactga taaagtcatc agcctgatta accagcaccg cgaaggcgtg gtgtttttgt
    86881 tgtggggatc acatgcgcaa aagaaagggg cgattataga taagcaacgc catcatgtac
    86941 tgaaagcacc gcatccgtcg ccgctttcgg cgcatcgtgg attctttggc tgcaaccatt
    87001 ttgtgctggc aaatcagtgg ctggaacaac gtggcgagac gccgattgac tggatgccag
    87061 tattaccggc agagagtgag taaatttgcg gggaaatgcc ggatggcaga gttgccaccc
    87121 ggctgattta tcaggcttta ttctgacgcc accattcacc aagcaaaacg ccggttgcga
    87181 cagagatatt cagcccggca acgttgcccg taccgtcaat cttcacgcgc agatcgttcg
    87241 gatcgcgtgc ggcatccggt aacccttcat attcctgacc cagcaccagt accattttcg
    87301 ctggcagact agttttgaac agcggtttac cctgctcgct ggaagtggtc actacggtgt
    87361 aacctgcctg acggaaatca tccagcacgt taacaatgtt gtcgccagta atcggctgaa
    87421 cgtgctctgc gccgccttct gcggtacgga tagccgcccc cgactccagc agtgccgcat
    87481 cctgcaccac gacacctttc acgccgaagt gcgcgcagct gcgcatcatg ccacccaggt
    87541 tatgcgggtt agattcgttt tccagtgcca gaacgcaatc ctgcgcgcct gcctggctta
    87601 cccactgctg cacggttgta ccgttacgct ttttgatcaa gaagcaaacg ccgccgtgat
    87661 gttccgtgcc tgacgctttt gtcaattccg cttcatccac cacatggtac gctttgcggt
    87721 ttgctgccat ccagcgcaag gcttctttaa aacgcggcgt tacactctgg ataaaccagg
    87781 cgcgaacaat cgcttccgga cggctctgga aaagtgcctg acatgcattt tcgccgtaga
    87841 cgcgggtttc ttccgcacgc tgacgacgca acacttccgg atcaataaaa cttttaccac
    87901 tgatgccacc gtgatcggcc ttttccggcg tctcatcacc cggcgcgcgg gaaaccgtgc
    87961 gccacggcga atcttcccac ttgcggtcac ggggctgact ctgtttgtca tcgcgggcgg
    88021 ggcgacggcc accgtcagca cgagattttc ctggacgccc gccccctttc ccggtacgcg
    88081 ggttgtgggt acgtttatca gaatcatcat cactgcggac atacatcact ttgaccttgc
    88141 cgcttttacc tttcatttca tcgttcatgc ttttctccac cagcgctgcg cgaagcgcgc
    88201 agattacccg aagtccgcgc ggttcgccat gatttcgtac caaagcctgc gactatcata
    88261 cctattgaat aaaacagatt gttgtctgga acaatgtccc cgataatatg taacatatta
    88321 gaaacatacc ggcgtcgttg ccgataagtc tccttactca tcccgaggtt agttatgaat
    88381 accgtttgta cccattgtca ggccatcaat cgcattcccg acgatcggat cgaagatgcg
    88441 gcaaaatgcg gacgctgcgg tcacgacttg tttgacggag aggtgattaa tgcgaccggt
    88501 gaaacactcg acaaattgct gaaggatgat ctacctgtgg tgatcgactt ctgggcaccg
    88561 tggtgcggcc cctgccgtaa tttcgcacca atttttgaag atgtcgcgca agagcgtagc
    88621 ggtaaagtgc gctttgtgaa agtgaatacc gaagctgaac gtgaattgag cagtcgcttt
    88681 ggaattcgta gtataccgac gatcatgatt ttcaaaaacg gtcaggttgt cgacatgctt
    88741 aatggcgcag taccgaaagc gccgttcgat agctggctga acgaatctct ttaatcttac
    88801 cggggcgcat cttgtgcccc gttttctcct ctgcgacaat ggcgtttttt cgacgctctc
    88861 ttatgaccga aaacgctgtt ctccagttac gcgccgagcg tattgcgcgc gcaacacgtc
    88921 cttttcttgc ccgcggtaat cgcgttcgtc gctgccaacg ctgtcttttg ccagagaaat
    88981 tatgtctctg ttcgacaatt accccagcac aagcaaaaag tcgcttctgt ttgctgatgt
    89041 tcgacaccga gccaatgaag cccagtaata ccgggcgtct gattgctgat attttgcctg
    89101 ataccgttgc gtttcaatgg tcgcgtaccg aaccctcgca agatttactg gatctggtac
    89161 aaaacccgga ctatcagcca atggtggtct ttcccgcttc gtatgctgat gagcaacggg
    89221 aagtgatctt cacaccacct gccggtaagc caccgctgtt tatcatgctc gatggtacct
    89281 ggccggaagc tcgcaagatg tttcgtaaaa gtccgtatct ggataatctt cccgtcattt
    89341 ccgtcgatct ttcccggctt tctgcctatc gcctgcgtga agcccaggct gaaggccaat
    89401 attgtactgc cgaggtagcc atcgcactgt tagatatggc cggcgatacc ggggcggcag
    89461 cagggttagg cgagcatttt acccgcttta aaacacgcta tctggcagga aaaacgcaac
    89521 atctgggtag catcacagca gaacagttag aaagcgttta aaatcattcg gtcacttctg
    89581 cgggagaccg gtatgagtca gcgaggactg gaagcactac tgcgaccaaa atcgatagcg
    89641 gtaattggcg cgtcgatgaa acccaatcgc gcaggttacc tgatgatgcg taacctgctg
    89701 gcgggaggct ttaacggacc ggtactcccg gtgacgccag cctggaaagc ggtgttgggt
    89761 gtgttggcct ggccggatat tgccagcttg ccctttacac ccgaccttgc ggttttatgt
    89821 accaatgcca gccgtaatct tgctcttctg gaagagctcg gcgagaaagg ctgtaaaacc
    89881 tgcattattc tttccgcccc ggcatcgcaa cacgaagatc tccgcgcctg cgccctgcgc
    89941 cataacatgc gcctgcttgg accaaacagt ctgggtttac tggctccctg gcaaggtctg
    90001 aatgccagct tttcgcctgt gccgattaaa cgcggcaagc tggcgtttat ttcacaatcg
    90061 gcagccgtct ccaacaccat cctcgactgg gcgcaacagc gtgagatggg cttttcctac
    90121 tttattgcgc tcggcgacag cctggatatc gacgttgatg aattgctcga ctatctggcg
    90181 cgcgacagta aaaccagcgc catcctgctc tatctcgaac agttaagcga cgcgcgacgc
    90241 tttgtttcgg cagcccgtag tgcctcgcgt aataaaccga ttctggtgat taaaagcgga
    90301 cgtagcccgg cggcacagcg actgctcaac acgacggcag gaatggaccc ggcatgggat
    90361 gcggctattc agcgtgccgg tttgttgcgg gtacaggata cccacgaact gttttcggcg
    90421 gtggaaaccc ttagccatat gcgcccgctg cgtggcgacc ggttgatgat tatcagcaac
    90481 ggtgctgcgc ctgccgcgct ggcgctggat gccttatggt cacgcaatgg caagctggca
    90541 acgctaagcg aagaaacctg ccagaaactg cgcgatgcac tgccaggaca tgtggcaatc
    90601 tctaacccgc tcgatctgcg cgatgacgcc agcagtgagc actatattaa aacgctggac
    90661 attctgctcc acagccagga ttttgatgcg ctgatggtta ttcattcgcc cagcgccgct
    90721 gctcccgcaa cagaaagcgc gcaagtatta attgaagcgg taaagcatca tccccgcagc
    90781 aagtatgttt ctctgctgac gaactggtgt ggcgaacatt cctcgcaaga ggcacgacga
    90841 ttattcagcg aagccgggct gccgacctac cgtaccccgg aaggaaccat cactgctttt
    90901 atgcatatgg tggagtaccg gcgtaatcag aagcaactac gcgaaacgcc ggcgttgccc
    90961 agcaatctga cctccaatac cgcagaagcg catcttctgt tgcaacaggc gattgccgaa
    91021 ggagcaacgt cactcgatac ccatgaagtt cagcctatcc tgcaagcata tggcatgaac
    91081 acgctcccca cctggattgc cagcgatagc accgaagcgg tgcatattgc cgaacagatt
    91141 ggttatccgg tggcgctgaa attgcgttcg ccggatattc cacataaatc ggaagttcag
    91201 ggcgtcatgc tctacctgcg tacagccaat gaagtccagc aagcggcaaa cgccattttc
    91261 gatcgcgtaa aaatggcctg gccgcaggca cgggtccacg gcctgttggt gcaaagtatg
    91321 gctaaccgtg ctggtgctca ggagttgcgg gttgtggttg agcacgatcc ggttttcggg
    91381 ccgttgatca tgctgggcga aggcggtgtg gagtggcgtc ctgaagatca agccgtcgtc
    91441 gcgttaccgc cgctgaacat gaacctggcc cgctatctgg ttattcaggg gatcaaaagt
    91501 aaaaagattc gtgcgcgcag tgcgctacgc ccattggatg ttgcaggctt gagccagctt
    91561 ctggtgcagg tttccaactt gattgtcgat tgcccggaaa ttcagcgtct ggatattcat
    91621 cctttgctgg cttctggcag tgaatttacc gcgctggatg tcacgctgga tatctcgccg
    91681 tttgaaggcg ataacgagag tcggctggca gtgcgccctt atccgcatca gctggaagaa
    91741 tgggtagaat tgaaaaacgg tgaacgctgc ttgttccgcc cgattttgcc agaagatgag
    91801 ccacaacttc agcaattcat ttcgcgagtc accaaagaag atctttatta ccgctacttt
    91861 agcgagatca acgaatttac ccatgaagat ttagccaaca tgacacagat cgactacgat
    91921 cgggaaatgg cgtttgtagc ggtacgacgt attgatcaaa cggaagagat cctcggcgtc
    91981 acgcgtgcga tttccgatcc tgataacatc gatgccgaat ttgctgtgct ggttcgctcg
    92041 gatctcaaag ggttaggctt aggtcgacgc ttaatggaaa agttgattac ctatacgcga
    92101 gatcacggac tacaacgtct gaatggtatt acgatgccaa acaatcgtgg catggtggcg
    92161 ctagcccgca agctcgggtt taacgttgat atccagctcg aagaggggat cgttgggctt
    92221 acgctaaatc ttgcccaacg cgaggaatca tgagtaaggt actggaaatg ttgaccactt
    92281 aatcgggact ggtgttatta ttgcccgctt atgtcgtctg cattgcacag aggacccttc
    92341 aatgaacaga gaagaaatgc actgtgatgt tgtcaaaatt taagcgtaat aaacatcaac
    92401 aacaccttgc ccaactaccc aagatttctc aatcagttga tgatgtcgat ttcttttacg
    92461 ctcccgccga cttccgggag acgctgctgg aaaaaatagc cagcgcgaag cagcgcattt
    92521 gcattgtcgc cctgtatctc gaacaggatg acggtggcaa aggcattctg aacgcgttgt
    92581 atgaagctaa aaggcagcgt ccggaactgg atgtgcgggt gctggtcgac tggcatcgtg
    92641 cacaacgtgg acgcattggc gctgcggcat ctaacactaa cgctgactgg tactgccgca
    92701 tggcgcagga aaatccgggc gtagatgttc cggtttatgg cgttccaatc aatacccgtg
    92761 aagcccttgg tgttctgcac tttaaaggct ttatcatcga cgatagcgta ctttatagcg
    92821 gtgccagcct gaacgatgtt tacctgcatc agcacgataa atatcgctac gaccgttatc
    92881 atctgatccg taaccgtaag atgtcagaca ttatgtttga atgggttaca cagaatatta
    92941 tgaatggccg cggcgttaat cgtctggatg atgttaatcg gccaaaaagc ccggaaatca
    93001 agaacgatat tcgtctgttc cgccaggagc tgcgtgatgc cgcttatcat ttccagggcg
    93061 atgccgacaa cgatcagctt tccgtaacgc cgctagtggg gctggggaaa tcgagtctgt
    93121 tgaacaagac cattttccat cttatgcctt gtgccgagca gaaactaacc atctgtacgc
    93181 catacttcaa cctgccagca atccttgtgc gcaatattat ccagttgctg cgcgaaggga
    93241 aaaaggtcga aattattgtt ggtgataaaa ccgcgaacga cttctacatt ccggaagatg
    93301 aacctttcaa gataattggc gcattgcctt atctctatga gatcaatctg cgtcgtttcc
    93361 tgagccgttt gcagtattac gtcaatactg accagctagt ggttcggtta tggaaagatg
    93421 acgacaacac ctatcaccta aaagggatgt gggttgatga taagtggatg ttgatcaccg
    93481 gtaataacct gaacccgcgc gcctggcgtc tggatctgga aaacgccatt ttgatccacg
    93541 atccacaact ggagcttgcg ccgcagcgag agaaagaact ggagctgatc cgcgagcata
    93601 ccaccatcgt taagcactat cgcgatctgc aaagtattgc cgattatccg gtgaaggttc
    93661 gtaaactcat ccgccgtttg cgccgtatcc gcatcgaccg attaattagc cgcatcctgt
    93721 aatcacaacc ccgtcctgta cggggtttgt tttttggagg ccacgttttg cgtattcctt
    93781 ttgtctgttc gctattgtta ctttctggat gcagccatat ggctaacgat agctggagcg
    93841 ggcaggataa agctcaacac tttatcgcct cggcgatgct ttccgccgcc ggaaatgaat
    93901 attcacagca tcaggggatg agccgggatc gcagtgccat gtttggattg atgttctctg
    93961 tcagtttggg ggcgtcaaaa gagctttggg atagccgccc cgaagggagc ggctggagct
    94021 ggaaggattt ggcctgggat gtcgccggtg caagcaccgg ctataccatc tggcaactga
    94081 cccgtcacta aagacgcatc cccttccctt tgcgatgtag catcaaagaa accagaaacg
    94141 ccaccacggc catcaaggtc acataccaga agaaggctgt ttccattcct attgatttca
    94201 gcgacaacgc tacgtactcc gccgaaccac caaatatagc attagcgacc gcatatgaca
    94261 gaccaacgcc taatgcgcga acctgtgccg ggaacatctc agccttcagt attccactga
    94321 ttgatgtata aaaactcact atcagcaggg cacacatcac cagaccaaaa gcggcataag
    94381 gcgaggaaac gttttgcaat gctgagagaa taggaacggt aaaaatggct gccagcgaac
    94441 cgaaacataa cattgaggta cggcgaccaa tcttatccga cagcgcgcca atgagtggtt
    94501 gaataagcat gaatacaaac aatgcggcag tcataatgcc actcgccacg ttggcatgca
    94561 ttcccgcagt atttaccaga tacttctgca tataagtggt gaaggtatag aaacaaaggg
    94621 agcccgcagc ggtaaaaccg agaaccatga tgaatgcacg gcgattgcgc cataatcctt
    94681 tcagagatcc agcttctttt aaagcgcgcg tttcttgttg cgaagtttca tctaactgac
    94741 gacgtaacca caacgccaca acagctaaca cagctcctaa cgcgaaagga atacgccatc
    94801 cccactctct gagtgcagcg tcttccatgg tgtgttgtaa aaccacgaca accagtaggg
    94861 ctagcagttg tccgccgatc aacgtcacat actgaaatga tgcgtaaaaa cctttgcgcc
    94921 cttcaacggc aacttcactc atataggtgg cgctggtgcc atattctccg ccaacagata
    94981 atccctgaaa taaacgcgcg agaagcaata atgccggagc ccacgtacct atagtttcat
    95041 aacctgggag gcaggcgata accagcgatc cgaaacacat catacacacc gataacagca
    95101 tcgatttttt gcgaccatgt ttatcggcta tgcggccaaa tagccaaccg cctattgggc
    95161 gcatcaggaa tcccgcagca aaaacacctg ctgtttgtag tagttgagtc gtcgtgttcc
    95221 cggaagggaa gaaaatgtgg gcaaagtaga gtgaacagaa cgagtagaca tagaaatcga
    95281 accactcgac cagattacct gaagaggccc ccacaatcgc ccaaatgcgg cgacgagtat
    95341 cactacttgt cagtttgctg tctgccgtta cagtactttc agccatgcca ttatgtctcc
    95401 tgccgtaatc cgatgctttt gtcggtcgct tttgtttatt tcttttggta aagaaaatgt
    95461 tatacatttg ttgcgtatca ttatgcaacc ttaaccatga atttagttag caggaaaatg
    95521 gttattgagg agcttaagga taaatttctg gtgaggagaa cacgtatgga gatgggcaag
    95581 ttggggaagc cgtattcgct gctgaatctg gcatatgtgg gagtataaga cgcgcagcgt
    95641 cgcatcaggc atttttttct gc
//
LOCUS       DAZGXZ010000004        94499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000004 DAZGXZ010000000
VERSION     DAZGXZ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 94499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.004"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            123..665
                     /locus_tag="ACHKJF_000325"
     CDS             123..665
                     /locus_tag="ACHKJF_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0947751.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            748..1449
                     /gene="elfD"
                     /locus_tag="ACHKJF_000326"
     CDS             748..1449
                     /gene="elfD"
                     /locus_tag="ACHKJF_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0947752.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1474..4074
                     /gene="elfC"
                     /locus_tag="ACHKJF_000327"
     CDS             1474..4074
                     /gene="elfC"
                     /locus_tag="ACHKJF_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP0947753.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            4065..5135
                     /gene="elfG"
                     /locus_tag="ACHKJF_000328"
     CDS             4065..5135
                     /gene="elfG"
                     /locus_tag="ACHKJF_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0947754.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5147..5689
                     /gene="ycbU"
                     /locus_tag="ACHKJF_000329"
     CDS             5147..5689
                     /gene="ycbU"
                     /locus_tag="ACHKJF_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0947755.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5697..6212
                     /gene="ycbV"
                     /locus_tag="ACHKJF_000330"
     CDS             5697..6212
                     /gene="ycbV"
                     /locus_tag="ACHKJF_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0947756.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFSHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6178..6915
                     /gene="ycbF"
                     /locus_tag="ACHKJF_000331"
     CDS             6178..6915
                     /gene="ycbF"
                     /locus_tag="ACHKJF_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0947757.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            7026..8036
                     /gene="pyrD"
                     /locus_tag="ACHKJF_000332"
     CDS             7026..8036
                     /gene="pyrD"
                     /locus_tag="ACHKJF_000332"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP0947758.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8210..8752
                     /gene="zapC"
                     /locus_tag="ACHKJF_000333"
     CDS             8210..8752
                     /gene="zapC"
                     /locus_tag="ACHKJF_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP0947759.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8749..9858)
                     /gene="ycbX"
                     /locus_tag="ACHKJF_000334"
     CDS             complement(8749..9858)
                     /gene="ycbX"
                     /locus_tag="ACHKJF_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP0947760.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10102..12210
                     /gene="rlmKL"
                     /locus_tag="ACHKJF_000335"
     CDS             10102..12210
                     /gene="rlmKL"
                     /locus_tag="ACHKJF_000335"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP0947761.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12221..14128
                     /gene="uup"
                     /locus_tag="ACHKJF_000336"
     CDS             12221..14128
                     /gene="uup"
                     /locus_tag="ACHKJF_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0947762.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14258..15511
                     /gene="pqiA"
                     /locus_tag="ACHKJF_000337"
     CDS             14258..15511
                     /gene="pqiA"
                     /locus_tag="ACHKJF_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP0947763.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15516..17156
                     /gene="pqiB"
                     /locus_tag="ACHKJF_000338"
     CDS             15516..17156
                     /gene="pqiB"
                     /locus_tag="ACHKJF_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP0947764.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17153..17716
                     /gene="pqiC"
                     /locus_tag="ACHKJF_000339"
     CDS             17153..17716
                     /gene="pqiC"
                     /locus_tag="ACHKJF_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP0947765.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17972..18139
                     /gene="rmf"
                     /locus_tag="ACHKJF_000340"
     CDS             17972..18139
                     /gene="rmf"
                     /locus_tag="ACHKJF_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP0947766.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18209..18727)
                     /gene="fabA"
                     /locus_tag="ACHKJF_000341"
     CDS             complement(18209..18727)
                     /gene="fabA"
                     /locus_tag="ACHKJF_000341"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP0947767.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18796..20556)
                     /gene="ycbZ"
                     /locus_tag="ACHKJF_000342"
     CDS             complement(18796..20556)
                     /gene="ycbZ"
                     /locus_tag="ACHKJF_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0947768.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20742..21194
                     /gene="matP"
                     /locus_tag="ACHKJF_000343"
     CDS             20742..21194
                     /gene="matP"
                     /locus_tag="ACHKJF_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP0947769.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21270..22310)
                     /gene="ompA"
                     /locus_tag="ACHKJF_000344"
     CDS             complement(21270..22310)
                     /gene="ompA"
                     /locus_tag="ACHKJF_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP0947770.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22667..23176)
                     /gene="sulA"
                     /locus_tag="ACHKJF_000345"
     CDS             complement(22667..23176)
                     /gene="sulA"
                     /locus_tag="ACHKJF_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP0947771.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23395..24024
                     /gene="sxy"
                     /locus_tag="ACHKJF_000346"
     CDS             23395..24024
                     /gene="sxy"
                     /locus_tag="ACHKJF_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP0947772.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23987..26149)
                     /gene="yccS"
                     /locus_tag="ACHKJF_000347"
     CDS             complement(23987..26149)
                     /gene="yccS"
                     /locus_tag="ACHKJF_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP0947773.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26159..26605)
                     /gene="yccF"
                     /locus_tag="ACHKJF_000348"
     CDS             complement(26159..26605)
                     /gene="yccF"
                     /locus_tag="ACHKJF_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP0947774.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26728..28782
                     /gene="helD"
                     /locus_tag="ACHKJF_000349"
     CDS             26728..28782
                     /gene="helD"
                     /locus_tag="ACHKJF_000349"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP0947775.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28814..29272)
                     /gene="mgsA"
                     /locus_tag="ACHKJF_000350"
     CDS             complement(28814..29272)
                     /gene="mgsA"
                     /locus_tag="ACHKJF_000350"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP0947776.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29368..30030)
                     /gene="yccT"
                     /locus_tag="ACHKJF_000351"
     CDS             complement(29368..30030)
                     /gene="yccT"
                     /locus_tag="ACHKJF_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP0947777.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATTIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30203..30616
                     /gene="yccU"
                     /locus_tag="ACHKJF_000352"
     CDS             30203..30616
                     /gene="yccU"
                     /locus_tag="ACHKJF_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP0947778.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30661..30978)
                     /gene="hspQ"
                     /locus_tag="ACHKJF_000353"
     CDS             complement(30661..30978)
                     /gene="hspQ"
                     /locus_tag="ACHKJF_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP0947779.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(31036..32226)
                     /gene="rlmI"
                     /locus_tag="ACHKJF_000354"
     CDS             complement(31036..32226)
                     /gene="rlmI"
                     /locus_tag="ACHKJF_000354"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP0947780.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDIRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32321..32599
                     /gene="yccX"
                     /locus_tag="ACHKJF_000355"
     CDS             32321..32599
                     /gene="yccX"
                     /locus_tag="ACHKJF_000355"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP0947781.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLIEPHHPSGELADFRIH"
     gene            complement(32596..32925)
                     /gene="tusE"
                     /locus_tag="ACHKJF_000356"
     CDS             complement(32596..32925)
                     /gene="tusE"
                     /locus_tag="ACHKJF_000356"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP0947782.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(33016..33675)
                     /gene="yccA"
                     /locus_tag="ACHKJF_000357"
     CDS             complement(33016..33675)
                     /gene="yccA"
                     /locus_tag="ACHKJF_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP0947783.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33882..33969)
                     /locus_tag="ACHKJF_000358"
     tRNA            complement(33882..33969)
                     /locus_tag="ACHKJF_000358"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33933..33935),aa:Ser,seq:tga)
     gene            34396..35514
                     /gene="hyaA"
                     /locus_tag="ACHKJF_000359"
     CDS             34396..35514
                     /gene="hyaA"
                     /locus_tag="ACHKJF_000359"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP0947784.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            35511..37304
                     /gene="hyaB"
                     /locus_tag="ACHKJF_000360"
     CDS             35511..37304
                     /gene="hyaB"
                     /locus_tag="ACHKJF_000360"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP0947785.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            37323..38030
                     /gene="hyaC"
                     /locus_tag="ACHKJF_000361"
     CDS             37323..38030
                     /gene="hyaC"
                     /locus_tag="ACHKJF_000361"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP0947786.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            38027..38614
                     /gene="hyaD"
                     /locus_tag="ACHKJF_000362"
     CDS             38027..38614
                     /gene="hyaD"
                     /locus_tag="ACHKJF_000362"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP0947787.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            38611..39009
                     /gene="hyaE"
                     /locus_tag="ACHKJF_000363"
     CDS             38611..39009
                     /gene="hyaE"
                     /locus_tag="ACHKJF_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP0947788.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            39006..39863
                     /gene="hyaF"
                     /locus_tag="ACHKJF_000364"
     CDS             39006..39863
                     /gene="hyaF"
                     /locus_tag="ACHKJF_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP0947789.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAVTQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39997..41541
                     /gene="appC"
                     /locus_tag="ACHKJF_000365"
     CDS             39997..41541
                     /gene="appC"
                     /locus_tag="ACHKJF_000365"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP0947790.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQALRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            41553..42689
                     /gene="appB"
                     /locus_tag="ACHKJF_000366"
     CDS             41553..42689
                     /gene="appB"
                     /locus_tag="ACHKJF_000366"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0947791.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            42702..42794
                     /gene="cbdX"
                     /locus_tag="ACHKJF_000367"
     CDS             42702..42794
                     /gene="cbdX"
                     /locus_tag="ACHKJF_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP0947792.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42874..44172
                     /gene="appA"
                     /locus_tag="ACHKJF_000368"
     CDS             42874..44172
                     /gene="appA"
                     /locus_tag="ACHKJF_000368"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP0947793.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(44287..46467)
                     /gene="etk"
                     /locus_tag="ACHKJF_000369"
     CDS             complement(44287..46467)
                     /gene="etk"
                     /locus_tag="ACHKJF_000369"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP0947794.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(46487..46933)
                     /gene="etp"
                     /locus_tag="ACHKJF_000370"
                     /gene_synonym="yccY"
     CDS             complement(46487..46933)
                     /gene="etp"
                     /locus_tag="ACHKJF_000370"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP0947795.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46921..48060)
                     /gene="gfcE"
                     /locus_tag="ACHKJF_000371"
     CDS             complement(46921..48060)
                     /gene="gfcE"
                     /locus_tag="ACHKJF_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP0947796.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(48106..50202)
                     /gene="gfcD"
                     /locus_tag="ACHKJF_000372"
     CDS             complement(48106..50202)
                     /gene="gfcD"
                     /locus_tag="ACHKJF_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0947797.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(50202..50948)
                     /gene="gfcC"
                     /locus_tag="ACHKJF_000373"
     CDS             complement(50202..50948)
                     /gene="gfcC"
                     /locus_tag="ACHKJF_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0947798.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50945..51589)
                     /gene="gfcB"
                     /locus_tag="ACHKJF_000374"
     CDS             complement(50945..51589)
                     /gene="gfcB"
                     /locus_tag="ACHKJF_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP0947799.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(51696..52001)
                     /gene="gfcA"
                     /locus_tag="ACHKJF_000375"
     CDS             complement(51696..52001)
                     /gene="gfcA"
                     /locus_tag="ACHKJF_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFP0947800.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            52291..52988
                     /locus_tag="ACHKJF_000376"
     CDS             join(52291..52540,52540..52988)
                     /locus_tag="ACHKJF_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFP0947801.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRVTARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(53220..53432)
                     /gene="cspH"
                     /locus_tag="ACHKJF_000377"
     CDS             complement(53220..53432)
                     /gene="cspH"
                     /locus_tag="ACHKJF_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP0947802.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            53718..53930
                     /gene="cspG"
                     /locus_tag="ACHKJF_000378"
     CDS             53718..53930
                     /gene="cspG"
                     /locus_tag="ACHKJF_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP0947803.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            53941..54129
                     /gene="ymcF"
                     /locus_tag="ACHKJF_000379"
     CDS             53941..54129
                     /gene="ymcF"
                     /locus_tag="ACHKJF_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0947804.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            54104..54334
                     /gene="ymcE"
                     /locus_tag="ACHKJF_000380"
     CDS             54104..54334
                     /gene="ymcE"
                     /locus_tag="ACHKJF_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP0947805.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            54324..54497
                     /gene="gnsA"
                     /locus_tag="ACHKJF_000381"
     CDS             54324..54497
                     /gene="gnsA"
                     /locus_tag="ACHKJF_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP0947806.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(54545..55618)
                     /gene="yccM"
                     /locus_tag="ACHKJF_000382"
     CDS             complement(54545..55618)
                     /gene="yccM"
                     /locus_tag="ACHKJF_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0947807.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(55690..58434)
                     /gene="torS"
                     /locus_tag="ACHKJF_000383"
     CDS             complement(55690..58434)
                     /gene="torS"
                     /locus_tag="ACHKJF_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP0947808.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QRQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            58517..59545
                     /gene="torT"
                     /locus_tag="ACHKJF_000384"
     CDS             58517..59545
                     /gene="torT"
                     /locus_tag="ACHKJF_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP0947809.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQFATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(59518..60210)
                     /gene="torR"
                     /locus_tag="ACHKJF_000385"
     CDS             complement(59518..60210)
                     /gene="torR"
                     /locus_tag="ACHKJF_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP0947810.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            60340..61512
                     /gene="torC"
                     /locus_tag="ACHKJF_000386"
     CDS             60340..61512
                     /gene="torC"
                     /locus_tag="ACHKJF_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP0947811.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            61512..64058
                     /gene="torA"
                     /locus_tag="ACHKJF_000387"
     CDS             61512..64058
                     /gene="torA"
                     /locus_tag="ACHKJF_000387"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP0947812.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            64055..64654
                     /gene="torD"
                     /locus_tag="ACHKJF_000388"
     CDS             64055..64654
                     /gene="torD"
                     /locus_tag="ACHKJF_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP0947813.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(64747..65052)
                     /gene="cbpM"
                     /locus_tag="ACHKJF_000389"
     CDS             complement(64747..65052)
                     /gene="cbpM"
                     /locus_tag="ACHKJF_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP0947814.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(65052..65972)
                     /gene="cbpA"
                     /locus_tag="ACHKJF_000390"
     CDS             complement(65052..65972)
                     /gene="cbpA"
                     /locus_tag="ACHKJF_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP0947815.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            66232..67488
                     /locus_tag="ACHKJF_000391"
                     /pseudo
     CDS             66232..67488
                     /locus_tag="ACHKJF_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
     gene            67781..69022
                     /gene="agp"
                     /locus_tag="ACHKJF_000392"
     CDS             67781..69022
                     /gene="agp"
                     /locus_tag="ACHKJF_000392"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP0947816.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(69060..69287)
                     /gene="yccJ"
                     /locus_tag="ACHKJF_000393"
     CDS             complement(69060..69287)
                     /gene="yccJ"
                     /locus_tag="ACHKJF_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP0947817.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(69308..69904)
                     /gene="wrbA"
                     /locus_tag="ACHKJF_000394"
     CDS             complement(69308..69904)
                     /gene="wrbA"
                     /locus_tag="ACHKJF_000394"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0947818.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            70277..70450
                     /gene="ymdF"
                     /locus_tag="ACHKJF_000395"
     CDS             70277..70450
                     /gene="ymdF"
                     /locus_tag="ACHKJF_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0947819.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(70533..71861)
                     /gene="rutG"
                     /locus_tag="ACHKJF_000396"
     CDS             complement(70533..71861)
                     /gene="rutG"
                     /locus_tag="ACHKJF_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP0947820.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAIMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(71882..72376)
                     /gene="rutF"
                     /locus_tag="ACHKJF_000397"
     CDS             complement(71882..72376)
                     /gene="rutF"
                     /locus_tag="ACHKJF_000397"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP0947821.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(72387..72977)
                     /gene="rutE"
                     /locus_tag="ACHKJF_000398"
     CDS             complement(72387..72977)
                     /gene="rutE"
                     /locus_tag="ACHKJF_000398"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP0947822.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDRSKLFARLPRLSFEEACGLL"
     gene            complement(72987..73787)
                     /gene="rutD"
                     /locus_tag="ACHKJF_000399"
     CDS             complement(72987..73787)
                     /gene="rutD"
                     /locus_tag="ACHKJF_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP0947823.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSITQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(73795..74181)
                     /gene="rutC"
                     /locus_tag="ACHKJF_000400"
     CDS             complement(73795..74181)
                     /gene="rutC"
                     /locus_tag="ACHKJF_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP0947824.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTVNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            complement(74193..74884)
                     /gene="rutB"
                     /locus_tag="ACHKJF_000401"
                     /pseudo
     CDS             complement(74193..74884)
                     /gene="rutB"
                     /locus_tag="ACHKJF_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
     gene            complement(74884..75975)
                     /gene="rutA"
                     /locus_tag="ACHKJF_000402"
     CDS             complement(74884..75975)
                     /gene="rutA"
                     /locus_tag="ACHKJF_000402"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP0947825.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            76263..76901
                     /gene="rutR"
                     /locus_tag="ACHKJF_000403"
     CDS             76263..76901
                     /gene="rutR"
                     /locus_tag="ACHKJF_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP0947826.1"
                     /translation="MTQGAVKTTGKRSRTVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            complement(76941..80903)
                     /gene="putA"
                     /locus_tag="ACHKJF_000404"
     CDS             complement(76941..80903)
                     /gene="putA"
                     /locus_tag="ACHKJF_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0947827.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAAQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSSHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            80958..81167
                     /locus_tag="ACHKJF_000405"
     CDS             80958..81167
                     /locus_tag="ACHKJF_000405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947828.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKINQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            81326..82834
                     /gene="putP"
                     /locus_tag="ACHKJF_000406"
     CDS             81326..82834
                     /gene="putP"
                     /locus_tag="ACHKJF_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP0947829.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            83377..84207
                     /gene="efeU"
                     /locus_tag="ACHKJF_000407"
     CDS             83377..84207
                     /gene="efeU"
                     /locus_tag="ACHKJF_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP0947830.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            84265..85392
                     /gene="efeO"
                     /locus_tag="ACHKJF_000408"
     CDS             84265..85392
                     /gene="efeO"
                     /locus_tag="ACHKJF_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP0947831.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            85398..86669
                     /gene="efeB"
                     /locus_tag="ACHKJF_000409"
     CDS             85398..86669
                     /gene="efeB"
                     /locus_tag="ACHKJF_000409"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP0947832.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            87288..88076
                     /gene="phoH"
                     /locus_tag="ACHKJF_000410"
     CDS             87288..88076
                     /gene="phoH"
                     /locus_tag="ACHKJF_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP0947833.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(88126..88539)
                     /gene="pgaD"
                     /locus_tag="ACHKJF_000411"
     CDS             complement(88126..88539)
                     /gene="pgaD"
                     /locus_tag="ACHKJF_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP0947834.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(88541..91849)
                     /gene="pgaB"
                     /locus_tag="ACHKJF_000412"
     CDS             complement(88541..91849)
                     /gene="pgaB"
                     /locus_tag="ACHKJF_000412"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011177.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP0947835.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKILCLVLCIPLCV
                     AYFHSGELMMRFVFFWPFFMSIMWIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISI
                     IIPCFNEEKNVEETIHAALAQRYENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHL
                     AQNQGKAIALKTGAAAAKSEYLVCIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRI
                     RTRSTLVGKIQVGEYSSIIGLIKRTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMI
                     TEDIDISWKLQLNQWTIFYEPRALCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRL
                     WRKENFRMWPLFFEYCLTTIWAFTCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILL
                     CTLCLLQFIVSLMIENRYEHNLTSSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQ
                     RARWVSPDRGILRG"
     gene            complement(91858..94281)
                     /gene="pgaA"
                     /locus_tag="ACHKJF_000413"
     CDS             complement(91858..94281)
                     /gene="pgaA"
                     /locus_tag="ACHKJF_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP0947836.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIK
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            complement(94288..>94499)
                     /locus_tag="ACHKJF_000414"
     CDS             complement(94288..>94499)
                     /locus_tag="ACHKJF_000414"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947837.1"
                     /translation="RIPEDPHHWNGFSGEKRILYGHSAHHFFFSSSTIHVSLPRLITD
                     YEVQKYLSFQLPVIRYRERFWQYME"
BASE COUNT        23352 a        23419 c        24652 g        23076 t
ORIGIN      
        1 aaagatttgt cacttattgg atttagtatg cttgatttaa atggcggtca attattgtta
       61 ttattaagtc taatgtcatt taaaggcatt atcatattta cgaagagatc aggatgatga
      121 cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg tccagcgtta
      181 tggcagctga tgataatgct atcacggatg gcaaagtaac atttaatggt aaagttattg
      241 ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg ccaaatgtca
      301 gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat gtaccaattg
      361 ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt agtggtacgg
      421 ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca gcgacgaatg
      481 ttgctctaca aatgtatctg ccagatggtt ctacatcggt aactcccggt accgaaacgt
      541 cgaatattca gttagcggat tctgctgagc aaacagtgac ctttaaagtt gattatatcg
      601 ctacggggaa agcgacttct ggtaatgtga atgcggtaac aaatttccat attaactatt
      661 attaatagaa ctcattaatt tttttattaa ttagtacccc tctagtgttc tggaggggat
      721 attcatattt tttaagagtg actatttatg aaaacttgca taacaaaggg aattgtgacc
      781 gtaagtttaa cggcaatatt actcagctgc tcatccgcat gggcggcagg taaaggcggg
      841 attggacttg cagcaacacg tcttgtttat tcagaaggtg aagagcaaat ttcactgggt
      901 gtacgtaaca ccagtccgga tgttccttat cttattcagt catgggtgat gaccccagat
      961 aataaaaaat cagcagattt tattattaca ccaccgctat ttgtgctgaa tccggctaat
     1021 gagaatctgt tacgcattat gtacattggt gcgccgttgg cgaaagacag agaaaccctt
     1081 ttcttcacta gcgtacgggc agtcccttca acaacgaagc gggaagaggg aaataccctg
     1141 aagattgcca cacaaagcgt catcaaactt ttctggcgac caaaaggttt agcgtatccc
     1201 ttaggcgagg ctccggcgaa actgcgttgc acttcgtcag ctgacatggt tacggtcagt
     1261 aacccaacac cttatttcat taccctgaca gacctgaaaa taggtggaaa agtagttaaa
     1321 aatcaaatga tttccccctt tgataaatac caattttctc tgccaaaggg ggccaaaaat
     1381 agcagcgtaa cgtatcgaac catcaatgac tacggggcgg aaacgccgca actcaactgt
     1441 aaatcgtaag ccgtcttcag ttaagagagc gagatgtata gaactcatcg acaacacagc
     1501 ctgttaagct ctggtggagt gccatcgttt attggtgggc tggtggtgtt tgtgtcggca
     1561 gcgttcaatg cacaagctga aacctggttc gatcctgctt ttttcaaaga tgatccctca
     1621 atggtggccg atttgtctcg tttcgaaaaa ggacaaaaaa taacgccagg ggtttatcgt
     1681 gtcgatattg ttctgaatca gacaattgta gatacgcgca acgtcaattt tgttgagata
     1741 acgccagaga aggggattgc cgcctgtttg acgactgaaa gcctggatgc aatgggtgtt
     1801 aatactgatg cgtttccggc ttttaaacaa ctggacaaac aagcgtgtgt gccattggcg
     1861 gagattattc cggatgccag cgtaacgttt aatgtgaata aactccgtct ggaaatttca
     1921 gtaccgcaaa tcgccatcaa aagtaatgcc cgtggttatg tcccccctga acgttgggat
     1981 gaagggatca acgcgctatt actgggatat tcgtttagcg gggctaacag tattcatagc
     2041 agcgcagaca gtgattctgg cgacagctat tttctgaatt taaacagtgg cgttaattta
     2101 ggcccatgga gattgcgcaa caattcaaca tggagtcgca gtagtggcca aaccgcagaa
     2161 tggaagaatc tcagcagcta tttgcagcgg gcggttattc cactaaaagg cgaactgacc
     2221 gtaggtgatg attatactgc aggcgatttt ttcgatagtg tcagctttcg tggtgtgcag
     2281 ctggcgtcag atgacaacat gctgccagac agcctgaaag ggtttgcgcc tgtggtgcgt
     2341 ggtatcgcca aaagcaatgc ccagataacg attaagcaaa atggttacac catttaccaa
     2401 acttatgtat cgcctggtgc ttttgaaatt agtgatctct attccacgtc gtcgagcggt
     2461 gatttgttag ttgaaatcaa agaagcggac ggcagcgtca atagctacag cgtaccgttt
     2521 tccagcgtgc cattactcca gcgtcagggg cgaatcaaat acgcggtgac actggcgaaa
     2581 tacagaacca atagtaatga acagcaggag agcaaatttg cccaggccac gttgcagtgg
     2641 ggcggaccgt ggggaacgac atggtatggt ggtggacaat atgctgaata ttaccgtgcc
     2701 gccatgtttg gtctgggatt taaccttggc gatttcggag caatttcgtt cgatgcgacc
     2761 caggcgaaga gtacgctggc agaccaaagc gaacataaag gtcagtcata tcgttttctg
     2821 tatgccaaaa cgctcaacca attgggcact aattttcaat tgatgggcta tcgctattcg
     2881 acgtcgggtt tctacaccct ttccgacacc atgtataaac atatggatgg ctacgaattt
     2941 aatgacggtg atgatgaaga tacgccgatg tggtcgcgtt attacaattt gttttacacc
     3001 aaacgtggca aactgcaggt caatatctcc cagcaattag gcgagtacgg ttcgttttat
     3061 ttaagtggta gccagcaaac ttactggcat accgatcaac aggatcggct attacagttt
     3121 ggctacaaca cgcaaattaa agatctctcg ctgggggttt cctggaacta cagtaagtcc
     3181 cgtggtcaac ctgatgctga tcaggtgttt gcactaaatt tttccctgcc gctcaatctg
     3241 ttgctcccca gaagtaatga tagctatacc aggaaaaaaa attacgcctg gatgacctct
     3301 aacaccagta tcgataacga agggcacatt acacaaaacc tgggtttaac ggagacacta
     3361 ctcgatgacg gtaacctgag ctacagcgtg caacagggat ataacagcga ggggaaaacg
     3421 gctaatggta gcgccagcat ggactacaaa ggggcgtttg cagatgcccg agtgggctac
     3481 aactacagcg ataacggcag tcaacaacaa ctgaactacg ctctttcagg cagtttagtt
     3541 gcccattcac agggcattac cctggggcaa tcgctggggg aaactaacgt gctgattgca
     3601 gcaccaggcg cagagaatac tcgtgtggcg aacagcaccg ggctgaaaac tgactggcgc
     3661 ggatataccg ttgtccccta tgccacttct tatcgggaaa atcgaatcgc acttgatgcg
     3721 gcatcgttaa aacgtaacgt ggatcttgaa aatgcagtag tcaacgtggt tcccaccaaa
     3781 ggggcgttgg ttctggcgga gttcaatgcc catgcgggag caagggtatt aatgaaaaca
     3841 tcaaagcagg gtataccgct gcgttttggc gcgatagcga cgctggacgg catacagact
     3901 aatagcggca ttattgatga tgatggctcg ctctatatgt ccggtttgcc cgctcaggga
     3961 gcgataactg tacgctgggg cgaagctccc gatcaaattt gtcatatcag ttaccagctt
     4021 accgaacaac aaattaactc tgcgattacg cgaatggatg ccatatgcag ataatctttg
     4081 gagaaaaatg cgtgttatta ctacgactat tttttgccgc cgtcttaatg ctatggtgcg
     4141 ctcaaactgc tgcttatagc gggcagtgtc ataccactca ggggaatccg tatattggcg
     4201 tcaattttgg cgttaaaacc ctggaggaag aagaaaatac ggctggagta gttaaagaca
     4261 aattttatca gtggaacgaa tcgaatgatt attatgtttc ctgtgattgc gataaagaca
     4321 atgtcagaag tggccgatgg gcattcgccg cggattcacc gttagtctat ttaggcgaca
     4381 actggtacaa aattaatgac tatcttgccg ccaaagtttt attgcaggtt aaaggcagtt
     4441 ctcctactgc ggttcctttc gaaaacgtgg gcacaggggc agatacacga tggcatattt
     4501 gcgatcccgg cggtcaacgt ttaggtggcc agggggctag cggtaatagc ggtagctttt
     4561 ccctgaaaat attgcagccg ttcgttggtt cggtcgtcat tcctcctatg gcgctggcgc
     4621 gattatttga atgctacaac atacccgcag gtgattcctg cacgactaca ggtacatcgg
     4681 ttttagtgta ttacctgtct ggtacgatca attcacttgg ctcatgttcc gtcaatgccg
     4741 gagagactat cgaggtcgat ctgggcgacg tatttgcggc taactttcgt gttgtagggc
     4801 ataaacccct tggggccaga acggcagaac tcgcaattcc agtcaggtgt aacacgggaa
     4861 acgcggggtt agttaacgtc aacctgagtc tgacggcaac cacagacccc agctatcccc
     4921 aggcgattaa gacgtcacgt cctggcgtgg gcgtggtggt gaccgatagc cagaacaaca
     4981 ttatttcccc tgctggtgga acattaccgc tctctattcc tgatgatgca gacagtatcg
     5041 cgcgaatgaa tgtctatcca gtcagcacga caggtgtacc accagaaacc gggcgatttg
     5101 aagccacggc aacggtgaga ataaattttg attaacgcgt gaacgtatga agaaaaaaac
     5161 gatatttcag tgcgttatct tgttctttag cattcttaac atccatgtcg ggatggctgg
     5221 gcctgaacaa gttagtatgc atatttatgg gaatgtggtc gatcagggct gtgatgtcgc
     5281 caccaaaagt gcattacaaa atattcatat tggtgatttt aatatcagtg attttcaggc
     5341 cgcgaatacc gtaagcactg ctgctgattt gaatatcgat atcaccggtt gtgccgctgg
     5401 tattactggc gcggacgtcc tttttagcgg cgaggctgac acccttgcgc cgacactgct
     5461 caaactaact gacacaggcg gaagcggtgg tatggcaacg gggattgccg tgcaaattct
     5521 tgatgcgcaa agtcagcaag aaatcccgct caatcaggtc cagcctctta cgcccttaaa
     5581 agccggggat aacacactca aatatcaact tcgttataag tccacaaagg cgggagcaac
     5641 gggcggtaat gcgacggcgg ttctctattt tgatctggtt taccagtgaa ggagtgatgt
     5701 tgaaacggat aatctggatt ctgttcttac tgggattaac ggggggctgt gagctatttg
     5761 cccatgatgg cacggtcaac attagcggat cgtttcgccg taatacatgc gtgctggcac
     5821 aggatagcaa gcaaatcaat gtgcagttag gcgatgtttc gctgacacgt ttttctcatg
     5881 ggaattatgg cccggagaaa tctttcatca ttaatttgca ggattgcgga actgatgtca
     5941 gcacggttga cgtcaccttt tcaggaaccc ccgacggagt gcagagcgaa atgctgtcca
     6001 ttgaaagtgg tacggatgca gccagcgggc tggcaattgc gattctggat gatgcgaaga
     6061 tattgattcc gcttaaccag gcgagtaaag actacagcct acatagcggt aaggtgccat
     6121 taacttttta tgcgcaattg cgacctgtca atagcgatgt gcagtcgggt aaagtgaatg
     6181 ccagcgcgac atttgtactt cattatgact aatacgtgga acagattggc gctcttgatt
     6241 ttcgccgttt tatcgctgct ggtggcgggt gaattgcagg caggagtggt ggtcggcgga
     6301 acgcgattta tctttccggc agacagagaa tcgatatcta ttttactgac taataccagt
     6361 caggaatcct ggcttattaa cagcaaaatc aaccgcccaa cgcgttgggc cgggggggaa
     6421 gcgtcgacag tgccagcacc attactggcc gctccgccac ttattctcct gaagcccggt
     6481 actacaggca cgttgcgctt gctgagaacg gaaagcgaca tcttgcctgt ggatcgcgaa
     6541 acgctatttg agttaagcat tgccagcgtg ccatccggca aagttgaaaa tcagagcgta
     6601 aaagtggcga tacgctcggt atttaaactg ttctggcgac ccgaaggttt gccgggcgac
     6661 ccgctggaag cttatcaaca attacgctgg acatggaatt cgcagggtgt acaactcact
     6721 aacccaacgc cttactacat taacctgatt caggtgagcg taaatggtaa agcgttaagt
     6781 aatgcgggag ttgtgccgcc taaaagccag cgtcagacaa gctggtgtca ggcaatcgca
     6841 ccgtgtcatg tcgcctggcg ggcgataaat gattacggcg ggttgagtgc aaagaaggag
     6901 caaaatctgc cctgaaacag gttcggaaaa cgtttgcgtt ttttttgccg caggtcaatt
     6961 cccttttggt ccgaactcgc acataatacg cccccggttt gcacaccggg aatccaggag
     7021 agttcatgta ctaccccttc gttcgtaaag cccttttcca gctcgatcca gagcgcgctc
     7081 atgagtttac ttttcagcaa ttacgccgta ttacaggaac gccgtttgaa gcactggtgc
     7141 ggcagaaagt gcctgcgaaa cctgttaact gcatgggcct gacgtttaaa aatccgcttg
     7201 gtctggcagc cggtcttgat aaagacgggg agtgcattga cgcgttaggc gcgatgggat
     7261 ttggatcgat cgagatcggt accgtcacgc cacgtccaca gccaggtaat gacaagccgc
     7321 gtctctttcg tctggtagat gccgaaggtt tgatcaaccg tatgggcttt aataatcttg
     7381 gcgttgataa cctcgtagag aacgtaaaaa aggcccatta tgacggcgtc ctaggtatta
     7441 acatcggcaa aaataaagat acgccagtgg agcagggcaa agatgactat ctgatttgta
     7501 tggaaaaaat ctatgcctat gcgggatata tcgccatcaa tatttcatcg ccgaataccc
     7561 caggattacg cacactgcaa tatggtgaag cgctggatga tctcttaacc gcgattaaaa
     7621 ataagcaaaa tgatttgcaa gcgatgcacc ataaatatgt gccgatcgca gtgaagatcg
     7681 cgccggatct ttctgaagaa gaattgatcc aggttgccga tagtttagtt cgccataata
     7741 ttgatggcgt tattgcaacc aataccacac tcgatcgttc tcttgttcag ggaatgaaaa
     7801 attgcgatca aaccggtggc ttaagtggtc gtccgcttca gttaaaaagc accgaaatta
     7861 ttcgccgctt gtcactggaa ttaaacggtc gcttaccgat catcggtgtt ggcggcatcg
     7921 actcggttat cgctgcgcgt gaaaagattg ctgcgggtgc ctcactggtg caaatttatt
     7981 ctggttttat ttttaaaggt ccgccgctga ttaaagaaat cgttacccat atctaattat
     8041 ttcttcgaat tcctctttat aagataatca gggctttatt ttcagccctg gttgttttat
     8101 attcattgct gttgcttatt tagacatttt gtacttttat tgttgaggtt attaagcgaa
     8161 gcgacaatgg attgtgttgc tgcggtttta tagggtaggg gagaggcaga tgcgaattaa
     8221 accagacgat aactggcgtt ggtattacga tgaagagcac gatcgaatga tgctcgattt
     8281 agccaatggt atgctatttc gctcacgttt tgcgcgcaag atgttgactc cagatgcctt
     8341 ttctcccgca ggcttttgcg ttgacgacgc cgcgctctat ttctcttttg aagaaaagtg
     8401 ccgcgatttt aatttatcta aagaacaaaa agccgaactg gtgctgaatg cactggtagc
     8461 aattcgctat ctcaaaccgc aaatgccgaa aagctggcat tttgtttccc atggtgaaat
     8521 gtgggtgccc atgccgggcg atgcggcctg cgtttggttg agcgatactc acgagcaggt
     8581 caatttgctg gtcgttgaat ctggcgaaaa tgccgcacta tgcctgctgg cacaaccctg
     8641 cgttgttatt gcgggtcgcg cgatgcagtt gggcgacgcc attaaaatca tgaacgacag
     8701 gctgaaaccg caggttaatg ttgacagctt cagcctcgaa caggcagtct aacgcgccaa
     8761 cttaagtgca gtcttcggta cacagctaca gcaaagaatg gtgccatcat cgcccattgc
     8821 tgattttttc agcggcgtga cttcgccttc taaaagctga acacggcaac ttccacaaat
     8881 gcccgcgcgg caagaataag ggatacgaat tccctgattt tctaattgct ccagcaacac
     8941 ctgttggtta tttccacgaa atgcctgtcc ctgccagtca atatctacat ttgcgtccgg
     9001 ttgttgcgtg atgttggcgg tatcatcagc ggcagctgcg ccgtaaattt ttgccggagc
     9061 cgttgccaga atttccacct catcgccaac gcgaatcacg ccgctattac gggcaattaa
     9121 attctgacca aaatcgacat cgccgttatc ctgggcagtg cggaaagatt gcaatgtttt
     9181 taatggttcg cctgccggat gtttttgccc tttttctggg ctgacggtgg tgaaaataca
     9241 gcggctacaa ggtttaacca catcaaacac cacatcacca atgcgaatca ctttccagcg
     9301 atcttcttcc caggctgacg cgccggaaac caccagattg gggcggaatt gctccatttt
     9361 tacactggcc ggacaacgtt gttggagatc acgtaacgag gcttcgttag caagaaggta
     9421 aggatagcca tcagcaaatg acagaggtac agtgttgtgg cgtttcacgc gccgagtcat
     9481 ttgtggcccc acccagcgta attgcacttc gcgggagaaa aatccactta gccatttgtt
     9541 gatcgcgtct ggcgcaattc gcgcggtaaa atgtgtgccc caaacttcgg ttggtgcgtc
     9601 ttgtgtggcg aaatcagcaa aacgaacata tgcgctactg ccatctggtg cggtgagatg
     9661 caagccatca tgcacaggtg aaggagtaaa ccgtaccatc tggggaaact ggcgagcggt
     9721 aataaacgta ccgtcaggct ccgtgatcat aaagatgcga tcgaaggcca gaccactgac
     9781 atcagccaga gcatgtgtaa gaccaatgcc gcgcatcgat ttaacaggat gaataaaaag
     9841 ccgggttaat gtcaccacgg tgcggtcctc aaatgaaaat aagccctcaa ctttatgaca
     9901 tgacgcgctt attagctata atgcgcaaca attttcttag cgcctgtccc attaggctat
     9961 tttatttgtc agtttggccc tgggttgtgc ttgaaatcct cacgtacttg tgtacgctcc
    10021 ggtttctccg cgctgtccat gtccagactg actgcaacaa ttacgcctac tgcggtaggc
    10081 tcttagagta aaagtgacga tatgaattct ctgtttgcca gtacggcccg tgggctggaa
    10141 gagctgttaa aaactgaact ggaaaacctg ggggccgttg aatgccaggt ggttcagggt
    10201 ggggtccatt tcaagggcga cacacggctt gtttaccaga gcctgatgtg gagccgcctg
    10261 gcctcgcgta ttatgttgcc gctgggcgag tgtaaggttt acagcgattt agacctctat
    10321 ctcggtgttc aggcgatcaa ctggacagag atgtttaatc ctggcgcgac cttcgctgtc
    10381 cacttcagtg gtttgaatga caccatccgc aacagtcagt acggcgcgat gaaagtgaaa
    10441 gacgcgatcg tcgatgcttt cacgcggaaa aatctgccgc gtccaaatgt tgatcgcgat
    10501 gcgccggata tccgcgttaa cgtctggctg cataaagaaa ccgccagtat cgctcttgat
    10561 ctgagtggtg atggtttaca tctgcgtggc tatcgcgatc gtgctggtat tgcgccgatc
    10621 aaagaaaccc tggcagccgc tattgtgatg cgatccggct ggcagccagg aacaccgctg
    10681 ctcgatccga tgtgtggttc cggtacgttg ctgattgaag cggcgatgct agcgaccgat
    10741 cgcgcaccag gcttgcaccg tgggcgttgg ggctttagcg gctgggtgca gcatgatgaa
    10801 gatatctggc aggaagtgaa agcggaagcg caaactcgtg cccgtaaagg cctggctgag
    10861 tatagctctc atttttacgg ttcggacagc gacgcacggg tgattcaacg tgcgcgcact
    10921 aacgcccgtc ttgcggggat tggtgaactg atcacctttg aggtgaaaga tgtcgcgcaa
    10981 ctgaccaatc cgctgccgaa agggccgtac ggcacagtgt tgagcaaccc gccatacggg
    11041 gaacgtctgg acagcgaacc ggcgctgatt gcgctgcata gcctgctggg tcggatcatg
    11101 aaaaaccagt ttggtggctg gaatctctct ttgtttagtg cctcgccgga tctgctaagc
    11161 tgcttgcagc tgcgtgcaga caaacagtac aaggcgaaaa acggcccgct ggactgcgta
    11221 cagaaaaatt accacgttgc cgaaagcact ccagacagca aaccggcgat ggtagcggaa
    11281 gactacgcca accgcctgcg taagaacctc aaaaaattcg agaagtgggc tcgccaggaa
    11341 ggaattgaat gttaccgcct gtatgacgcc gatctaccgg aatataacgt tgccgttgac
    11401 cgttatgccg actgggtggt ggtgcaggag tatgcgccgc caaaaactat tgatgctcac
    11461 aaagcgcgtc agcgtctgtt cgatattatc gctgcaacca tttcggtact ggggattgcg
    11521 ccaaacaaac tggtgctgaa aacccgtgaa cgccagaagg gcaaaaatca gtaccagaaa
    11581 ctgggcgaga agggcgagtt tcttgaagtt accgaatata acgctcactt gttggtgaac
    11641 ctgacggatt acctcgatac cggtctgttt ctcgatcacc gtatcgcccg tcgtatgctc
    11701 ggtcagatga gcaaaggcaa agatttcctc aacctgttct cttataccgg cagcgccacc
    11761 gtgcatgcgg gattaggcgg tgcacgcagc accaccaccg tggatatgtc gcgtacttat
    11821 ctggagtggg cagaacgcaa cctgcgtttg aatggcctga ccgggcgtgc gcatcgcctg
    11881 attcaggccg attgcctggc gtggctgcgt gaggcaaatg aacagttcga tctgatcttt
    11941 atcgatccgc caaccttctc taactcaaaa cgaatggaag atgcgtttga tgttcagcgc
    12001 gatcatctgg cgctgatgaa agatttgaaa cgtctgctgc gtgcaggtgg gacgattatg
    12061 ttctcgaaca ataaacgtgg cttccgtatg gatctcgacg gcctggcaaa actgggactg
    12121 aaagcacaag aaattacgca aaaaacgctc tcccaggatt tcgcccgtaa ccgccagatc
    12181 cacaactgct ggctgattac cgcagcctaa aggaatagta atgtcattaa tcagtatgca
    12241 tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat aacgcagaac tgcatatcga
    12301 agataacgaa cgtgtttgtc tggtgggccg caacggcgcg ggcaaatcga cgttaatgaa
    12361 aatcctcaac cgtgaacaag ggctggatga cggtcgcatt atttacgagc aagatttgat
    12421 tgtagctcgt ctgcaacagg atccgccgcg taacgttgag ggtagcgttt atgatttcgt
    12481 tgccgaaggc attgaagaac aagcggaata tctgaaacgc tatcacgata tttcgcgcct
    12541 ggtgatgaac gacccgagcg agaaaaatct caacgaactg gcgaaggttc aggaacagct
    12601 ggatcaccac aacctgtggc agctggaaaa ccgcatcaac gaagtgctgg cgcaactggg
    12661 gttagatcct aacgttgcgc tgtcgtcgct ttccggcggc tggttgcgta aagcggcatt
    12721 aggacgcgcg ctggtgagta atccgcgcgt gctgttgctt gatgaaccga caaaccacct
    12781 ggatattgaa accatcgact ggctggaagg gtttttgaaa actttcaacg ggacgattat
    12841 tttcatctcc cacgaccgtt cgtttatccg caatatggcg acgcgcattg ttgatctcga
    12901 tcgcggcaag ctggtgacct atccagggaa ttacgaccag tacctgctgg aaaaagaaga
    12961 agccctgcgc gtggaagaat tacaaaatgc cgagttcgat cgcaaactgg cgcaggaaga
    13021 ggtgtggatc cgccagggga tcaaagcacg ccgtacccgt aatgaaggcc gcgtacgcgc
    13081 cctgaaagcg atgcgtcgcg agcgtggtga acgtcgcgaa gtgatgggta ccgcaaagat
    13141 gcaggtggaa gaggccagcc gctccggtaa gatcgttttc gaaatggaag acgtttgcta
    13201 ccaggttgac ggtaagcaac tggtgaaaga tttttcggcc caggttctac gtggcgacaa
    13261 aattgccctg attggtccga atgggtgcgg caaaaccacg ctgctgaaac tgatgctcgg
    13321 tcagcttcaa gcggacagcg ggcgtattca cgttggcacc aaactggaag tggcttattt
    13381 cgatcagcac cgcgcggaac tggatcccga taaaacggtg atggataacc ttgccgaagg
    13441 taagcaagag gtgatggtta acggcaagcc acgccacgta ttgggctatt tgcaggactt
    13501 tctgttccat ccgaaacggg cgatgacgcc ggtacgtgcg ctttctggcg gtgagcggaa
    13561 ccgcttgctg ctggcgcgtt tgttcctcaa accaagcaac ttattgattc ttgacgaacc
    13621 gaccaacgat cttgatgtcg aaacgctgga actgctggaa gaactgatcg acagctatca
    13681 gggcacggta ttgctggtta gccacgatcg tcagtttgtc gataacaccg ttacagaatg
    13741 ttggatcttc gaaggcggcg gtaaaattgg tcgttatgtc ggcggttatc atgatgcccg
    13801 tggtcagcaa gagcagtatg tggcgctcaa acagcctgcg gtgaaaaaaa ccgaagaagc
    13861 cgccgcggca aaagcagaaa ctgtaaaacg cagcagtagc aaactaagct ataaattgca
    13921 gcgcgaactg gagcagctac cgcaattgct cgaagatctg gaggcgaagc tggaagccct
    13981 acagacgcag gtggcggatg cttccttctt cagtcagcca catgagcaga cacaaaaagt
    14041 gctcgccgat atggctgctg cagagcagga gctggagcaa gcctttgaac gctgggagta
    14101 tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt cgatttaaac agagacagct
    14161 gacaacggta aaattgcctg atgaactggg tttatcaggc atgaaaacca acactttgtt
    14221 agtcatctgt aaaatagcgc atcattaagg agtaccaatg tgcgaacatc atcatgccgc
    14281 gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg ttaccgcgcc ttgagcatgg
    14341 tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc gtggcgtggg atgcccctcg
    14401 gcagcgtccc accgcctatg cgttggctgc actgttcatg ctgttgctgt ccaacttgtt
    14461 tccttttgtg aatatgaacg ttgcaggagt taccagtgaa attacattac tggaaattcc
    14521 cggcgtgctt ttttctgagg actacgccag cctcggcacc tttttcctgt tgtttgtgca
    14581 actggttccc gcgttttgtc tgataaccat tctgttactg gtgaatcgcg cggaattacc
    14641 ggtccgttta aaagagcaac tggcacgggt gctttttcaa ctcaaaacct ggggaatggc
    14701 ggagattttc ctcgctggtg tgctggtcag tttcgttaaa ctgatggctt acggcagcat
    14761 tggggtaggc agcagctttc tcccctggtg tttattttgt gtcctgcaac tgcgcgcttt
    14821 tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc ccgatgccag aactgcgcca
    14881 gccgctaaaa ccaggcgtca cggggatacg tcaggggctg cgttcgtgct cctgttgtac
    14941 ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt agtaccaaag ggtacgttcg
    15001 gcgtagaaac agcctgcagt ggacactcgc gctgcttgta acgtccatca tgctgtatct
    15061 tccggctaat attttgccca tcatggtgac ggatttatta ggctcgaaga tgccgtcgac
    15121 gattctcgct ggggtcattc tgttatggag cgaaggctct tatcccgtcg ctgcggtgat
    15181 ctttctggcc agtattatgg tgccaacgtt aaagatgatc gccatcgcgt ggctgtgttg
    15241 ggatgccaaa gggcatggca agcgcgacag tgaaagaatg catttgattt atgaagttgt
    15301 tgagtttgta ggccgctggt cgatgattga cgttttcgtt atcgcggtgc tctcggcgct
    15361 ggtgcgtatg ggaggtttaa tgagtattta tccggcaatg ggtgcattaa tgtttgcttt
    15421 agtcgtcata atgacaatgt tttctgctat gacgtttgac ccgcgtttgt cgtgggatcg
    15481 tcaacctgaa tcagagcatg aggagtcctg acagtatgga atctaataat ggggaagcca
    15541 aaatccagaa agtgaagaac tggtctcccg tgtggatatt tcctatcgtc acggcgctca
    15601 ttggggcctg ggttcttttt tatcattaca gccatcaggg accggaagtg accctgatca
    15661 ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat taaaagccgt agcgttgacg
    15721 tcggcgtggt tgaaagcgcc acactggctg atgatttgac gcacgttgaa atcaaagcgc
    15781 ggctgaattc cggtatggaa aaattgctgc ataaagacac cgtcttttgg gtggtgaaac
    15841 cgcagattgg tcgcgaaggg attagcggcc tgggaacgct gctgtctgga gtttatatcg
    15901 aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata cgatttgctg gactcgccac
    15961 cgttggcccc gcctgatgcg aaaggtatcc gtgtgattct cgatagcaaa aaagccgggc
    16021 agctctcgcc aggagatccg gtgctgttcc gtggctatcg ggtaggttcg gttgaaacca
    16081 gcaccttcga tacacaaaaa cgcaatatca gctatcaact gttcatcaat gcaccttatg
    16141 accgactggt gaccaacaat gttcgcttct ggaaagatag tggcattgcg gttgatctga
    16201 cgtcagcagg gatgcgtgtg gagatgggct cattgacaac gctgctgagt ggcggtgtca
    16261 gctttgatgt gccggaaggt ctggatttag ggcagccagt ggcaccgaaa acagctttcg
    16321 ttttgtatga tgatcagaag agcattcagg attcgttgta caccgatcac attgattatc
    16381 tgatgttctt taaagattcg gtacgcggtc tgcaaccggg agctccggta gagttccggg
    16441 gtattcgcct gggtaccgta agcaaagtgc cattctttgc gccgaatatg cgtcagacat
    16501 ttaacgatga ttaccgtatt ccggtactga ttcgtatcga gccagagcgg ctgaaaatgc
    16561 agcttggcga aaatgcggat gttgttgagc accttggcga attgttgaaa cgtggtttac
    16621 gcggatcgct gaaaaccgga aacctggtca ctggtgcact gtatgttgat ctcgatttct
    16681 atccaaatac gcctgcaata accggtattc gtgaatttaa tggttatcag attatcccga
    16741 ccgttagcgg cggcctggcg caaatccagc aacgactgat ggaagcgttg gataagatca
    16801 acaaactgcc attgaatccg atgattgaac aggcaaccag tacgctttct gaaagtcagc
    16861 gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa caagatcctc gctagccagt
    16921 cgatgcagca gttgccgacg gatatgcagt caacgttgcg tgaattgaat cgcagcatgc
    16981 agggcttcca gcctggctcc gcagcctaca acaagatggt ggcggatatg cagcgccttg
    17041 atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct caatgagaag agtaacgcgc
    17101 tggtatttga agcgaaggac aaaaaagatc cagagccgaa gagggcgaaa caatgaaaaa
    17161 gtggctagtg acgattgcag cactgtggct ggtcggatgc agctccggcg aaattaataa
    17221 aaactattac cagttacctg tggtgcagag cggtacacaa agtaccgcca gccagggcaa
    17281 tcgtctgtta tgggtagagc aggtcactgt tcctgactat ctggcgggga atggtgtggt
    17341 ttatcaaacc agtgatgtga agtatgtgat tgccaacaac aacttgtggg ccagcccgtt
    17401 ggatcaacag ttgcgcaaca ccctggttgc caacctgagt acgcaactgc ccggctgggt
    17461 ggttgcctcc cagcctctgg gaagcgccca ggacacgctc aatgttaccg taacggagtt
    17521 taacggtcgc tatgatggca aggtcattgt cagtggtgag tggctgttga accaccaggg
    17581 acaactgatc aaacgtccgt tccgtctgga aggagtgcaa actcaggatg gttacgatga
    17641 gatggttaaa gtgctggccg gtgtctggag tcaggaagcc gcttctattg cacaagagat
    17701 aaagcgtcta ccttaattat aaagatttgt aaatataacc gtctccggta tgttgcctga
    17761 ggcggttttt ttgtttctaa cgtgcggaaa aatttgttcc tcttcacatt ttttgtacaa
    17821 ccgacatgcc cgtgtagctc acaaatatga cagtggcgtg aattttgcgc attgacggca
    17881 gttatgattc gcggtattgc ttaactgtga ttgcacattt agtaatcact gttttctttt
    17941 ccaccagaaa ccagtatgag ggaaacgagg catgaagaga caaaaacgag atcgcctgga
    18001 acgggcacat caacgtggtt atcaggccgg catcgccgga cgctcaaaag aaatgtgtcc
    18061 ctatcagacg ctgaatcaaa ggtcacaatg gctgggaggc tggcgagaag ccatggcgga
    18121 cagggtagta atggcctgat tctgtctctt taaaaagaaa cctccgcatt gcggaggttt
    18181 cgccttttga tactctgtct gattataatc agaaggcaga cgtatcctgg aacagaccga
    18241 ctttcaggtc gctggcggta tagataagac gaccatcaac cagcacttcg ccatccgcca
    18301 ggcccataat cagacgacgg ttaacaatgc gtttaaagtg aatacggtag gtcacttttt
    18361 tcgctgtcgg cagtacctga ccagtgaatt tcacttcgcc aacgcccagc gcgcggcctt
    18421 taccttcgcc gcccagccag ccgaggtaga accctaccag ctgccacatt gcgtccaggc
    18481 ccaggcatcc cggcataacc ggatcgccaa taaagtggca tccgaagaac cacagatccg
    18541 gattgatatc cagttctgct tcaacatacc ctttgtcgaa gttaccaccc gtttcggtca
    18601 ttttgaccac acggtccatc atcagcatgt tcggtgctgg caattgcggg cctttagcgc
    18661 caaacagttc accgcgacca gaggcaagaa ggtcttcttt tgtataggat tcgcgtttat
    18721 ctaccatgtt ctctgtaagc cttattttat tgaagcacgc aggatagcta acacgtgtac
    18781 gctgaacaag tccgatcagt tcggaataaa ccagttcagc caacgtaatg gccatggaaa
    18841 acggtgacgt ccttcctgtt gcgatgcttg cgcgatacgt tcctggatgg tttgcatcag
    18901 cgtcgtttgg ccttcgccat cccacaccag atttaataat aacggcagtg cgtcagtcac
    18961 atcgtctact gcccagatgg tgaatttgcc ttcttctacc gctttcacca gttcactgtg
    19021 aagacttaaa tggcgaacgt tagctgtggg gataatgaca ccttgtttcc cggttaactc
    19081 acgttgctgg caaatagcaa agaagccttc gattttctca tttaaaccac cgaccggctg
    19141 ggcgcgaccg aactgatcga ctgaacctgt gatagcgata ctctgattca ccggcacatc
    19201 ggcgagggcg cttatcaggg cgcagagttc agccatcgag gcactatctc catcaacttc
    19261 actgtatgac tgctcaaatg tcagcgatgc tgagaagggg atctgttgct caagctgtag
    19321 ttccgacatc aggaacgctt gcatgatcat catccctttc gcatggatat taccgccaag
    19381 ctccgctttg cgttcgatgt cggtgaattc accatcgcca atatgcacaa cgcagctaat
    19441 gcgagaaggt tcgccaaaag cgcgtggatg acccggaaat tcaatgaccg aaagggcgtt
    19501 aatttgcccg atgcgttcgc cttcggtttc aatcaggatt tgctcctgaa ggatctcatc
    19561 ctgcatacgt tcagcgagga aaccttcgcg ccattcacgc tgctgcagca ttaagtttag
    19621 ctgctcgccg gagaaggtgt cgccatcaca cagggaggcg acctctttac actggcggag
    19681 gatccactgc gggctaagcg gaagtgtttc ttgttcaccg gtgtagcgtg ctgcttcgcg
    19741 gataagtacc ggccaggcat ccgctcccgg tgcaggtaag tgattatgtc tggcggtaaa
    19801 tgtcacccag cgacaccact gggttactga ctccgcatcg acaatctgca gagtatcttc
    19861 aaattcgcta taaatagcct gctctgaaag ctctggctcc atctcctgga aatcagccaa
    19921 tgattcgcgt tcgcctacca gaatgacttt cagcttcaat ggcatcgaag gcacagagac
    19981 ggggagaggg cgcgactcat caaacgcaac ccagtcaaaa cgctcgcggt taacgatatt
    20041 tttcagccgc atccacagca gaggttgcgc cagcagtgta cgcaaagaga tgatgagaat
    20101 accgccattt gcctgatgca ccagaccagg ctgcagggta atgtcgccat taaactggcg
    20161 caggcagcca aagagttgct ccgcttctac ccagtcggca gcgacaactt gcgttaaagt
    20221 cgcaaaatta tcatctgcac tcactgcgtg acgtaagcgg atggagtggc cggaaacctc
    20281 atagtgaccg cccaccagtt ggcctgcatc gctttgtagc gtacgcgcgg cattggcaat
    20341 aagattcaga tactcagact cttccggggc cttcgccagc ataaaagagg aggatgctcg
    20401 cgtatgcagc aactgctcca gcgcaaattg cagccgcggt tgagtatcac tgaataaagg
    20461 atcgttttcg tcaatcaaat gtggctgagc aaatatttcc tgatagctat cggtatcagg
    20521 aaccaggtca cgccatgcaa gtttcgtaat ggtcaaagtt gatgtttttt agtctgttgt
    20581 taaagccgcg attataccgt aaccggcact acagcacacg tagaaagcac cgacaatact
    20641 cctggcatgg gcgttaaagc tcacaggatg gagattcttt tcttcactgg cctaaaaagc
    20701 tgatattctg taaagagtta cacggtaaca ttgagatcgc tatgaaatat caacaacttg
    20761 aaaatcttga aagcggttgg aaatggaagt atctggttaa gaagcatcgc gaaggggagt
    20821 taatcacccg ttacatagaa gccagtgccg cccaggaagc cgtcgatgtg ttgctctcgc
    20881 tggaaaatga acccgtgctg gtaaatggct ggattgacaa gcatatgaat ccggaactgg
    20941 tcaatcgcat gaagcaaacc attcgggcaa gacgaaagcg ccattttaat gcggaacacc
    21001 agcatacgcg caaaaaatcg atcgatctgg aatttattgt ctggcaacgt ctggctggtc
    21061 ttgcgcagcg tcgcggtaaa acgctttctg aaacgattgt tcagctgatt gaagatgcgg
    21121 aaaacaaaga gaaatacgcg aataaaatgt cttctctgaa gcaggatctg caggcattgc
    21181 tgggtaagga ataactgacg aaagtcagtt caatttacta aaggcaaaaa aaaccccgca
    21241 gcagcggggt ttttctacca gacgagaact taagcctgcg gctgagttac aacgtctttg
    21301 atacctttaa cttcgatctc tacgcgacga tccggagcca ggcagtcgat cagtgcagca
    21361 cgctgtttca cgttgtcaca ggtgttgcca gtaaccgggt tggattcgcc cataccacgt
    21421 gcggagatct tgtctgccgg gatacctttg gagatcaggt aatcaacaac agactgagca
    21481 cggcgctcgg acagaccctg gttgtaagcg tcagaaccga tgcggtcggt gtaacccaga
    21541 acaactacgg aaccgtcttt cggatccagg ttgctcagct ggctgtacag ctgatccaga
    21601 gcagcctgac cttccggttt cagggttgct ttgttgaagt tgaacagaac gtcagacttc
    21661 agagtgaagt gcttggtctg tacttccggt gccggagctg gagccggagc aactactgga
    21721 gctgcttcgc cctgaccgaa acggtaggaa acacccaggc tcagcatgcc gttgtccgga
    21781 cgagtgccga tggtgtgtgc gtcaccgatg ttgttggtcc actggtattc cagacgggta
    21841 gcgatttcag gagtgatcgc gtactcaaca ccgccagcga agaccggaga aacgccggtg
    21901 tcgtggtttt taccataaac gttggattta gtgtctgcac gccataccat accacccaga
    21961 cgagtgtaga tgtccaggtc gtcagtgatt gggtaaccca gtttagcggt cagttgaacg
    22021 ccctgagctt tgtatgcacc gttttcaacg ctgcctttgt acggcatacg acctaaccag
    22081 tcgtaaccca tttcaaagcc aacatacggg ttaacctggt aaccaccaaa agcaccagcg
    22141 cccagttggt tttcatgggt cgggccattg ttgttgatga aaccagtgtc atggtactgg
    22201 gaccagccca gtttagcacc agtgtaccag gtgttatctt tcggagcggc ctgcgctacg
    22261 gtagcgaaac cagccagtgc cactgcaatc gcgatagctg tctttttcat tttttgcgcc
    22321 tcgttatcat ccaaaatacg ccatgaatat ctccaacgag ataacacggt taaatccttc
    22381 accgggggat ctgctcaata ttaactctac cgatatcttc ggcttatgcc gagcacccct
    22441 agcgatgtaa agtctacaac gtagttgaaa acttacaagt gtgaactccg tcaggcatat
    22501 gaaaaaaaag tcttgtataa ggtatgttta atcttttttg tcagcgacaa tttacagaag
    22561 agaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa aattagtcac gactgaaagc
    22621 attggctggg cgacaaaaaa agttccagga ttaatcctaa atttacttaa tgatacaaat
    22681 tagagtgaat ttttagcccg gaaagttgtc tcgtggcgtg agaggatgcg cttaccggac
    22741 gcataataaa ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt
    22801 cttcagtcaa atcatctgcc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag
    22861 cgcgaaccat tgactccaca gtgtggcaag gggagagctg gctaatctgc attactttcg
    22921 ttaagggtag cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc
    22981 agagttgcca gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg
    23041 tcatcatggg ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt
    23101 ccgtagagac acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat
    23161 agcctgaagt gtacataatc aatccagccc ctgtgagtta ctgtatggat gtacagtaca
    23221 tccagtgaca acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg
    23281 tgacgggaaa acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta
    23341 acggatccgt taatgtgaat cattttttta tgttatgatt ttaaaaggaa ttttatgaaa
    23401 agcctctcct ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt
    23461 gaataccgat cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg
    23521 gtttctgatg gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa
    23581 catccgcctg tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat
    23641 cgggttgatg aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct
    23701 ctcgatgcag cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg
    23761 cccaatatgt cttttcatct ggaagcgatt ctcggggagg tggggattaa ggatgtacgg
    23821 gcgttacgta tacttggggc aaaaatgtgt tggttgcgac tgcggcagca aaacagtctg
    23881 gtgacagaaa agattctgtt tatgcttgaa ggtgccatta tcggcattca tgaagctgcg
    23941 ctcccggtgg cacgccgcca ggagcttgca gaatgggctg actctcttac gccgaaacag
    24001 gagtttcctg cggaacttga gtaatctggc gttgcagacg accaatctca ggcagtaatg
    24061 caatcaataa tccaacttgt tgtacgacca gaggttcttt gctgtctgcc cgtggttcaa
    24121 gttgctgcat ccgctgtttc aggctagcta atgcctcatt gacgcgttct tcatcagcag
    24181 gttgatgatg taacgcgtca tcaacatagc aaactgcgtc atcaagaaac gccagaattt
    24241 caggattagt taactgctcc cggtgagcac cgagggctga gatatagctg gtaaacgtat
    24301 ggttaaggca cagcaaccga aacgcggctt cgcgaatttg cggggtaacg ttcggctcgc
    24361 tggacatatt tgataccacc gacgccagct cagcatcacg gttgtgtgca tcgcggcggg
    24421 caatacgata cgccagacgg ttatcacgcc cctgatggta ttgctccagt atggcatcga
    24481 gataccgaca gttggcctct gtggcgcgtt cgagcatgcg cggcagattg cgaaactgcc
    24541 agtcaggcca gatgtagctc actgccgccc acgcaatggc acaaccaatc agcgtatcga
    24601 ttacgcgagg taacgctact tcaaaacctt cacccagtaa gttaaaacac agtagcacca
    24661 aaagtgtgat gaacatcgtt gcatgagcgt attgcacgtt acggaaggca aaaaagagca
    24721 cgccggtaat aaccagcagc accagctgcc cttccagtga tggcacaaac cacagcacag
    24781 gaatgccaat ggcgatacct accagcgtac caataatcct taacttcagg cggtggcgcg
    24841 tggcgttata gtttggctgg cagacaaaca aacttgtcag caagatccaa tacccgtgat
    24901 gcattccggt tatttgaatg atggcgtagc cgaagcacaa caccagcgac attcttaccg
    24961 catgacggaa gagggcggat tccggcgtga agtgacggct aagacgcagc cagatatcac
    25021 tcaacccgtg cgggctgtca tcagcgagct cattttcatc attattatgg ggtagtgcct
    25081 gggcctgttc tgattcaatt gttgccagtt gggcatcaat ggcgcgtaaa ttgttcagca
    25141 aaaatcccag tgttttgagt aaatcggcgg gtgcgccgtt atcgcgcatc cgctccagcg
    25201 cagcatcaat atgcgtaaaa gcgcgctcaa aatgcggatc atgttgataa ggctgacgca
    25261 acaaaataca gcgtgacagt tgctggcacg cctggccctg catcgacatc agccgctgaa
    25321 aacggaacag cacgtcgctg tggcgaaaat gttcacgcaa tgtttgatac tgaatatgag
    25381 aagagctggc acgctcgtga atatcctgtg cgacaaagta ataatgcagc gtgcgacgcg
    25441 ttccccgttg accacgatcg ccacgtaagc gggtcagcag cgagagtttc gtctgattca
    25501 atgtcgccat cagcagaccg ttggcgagag ccaaatcgta cagcggtgcc tggctttcat
    25561 cttcaatatc aggatcaaac atgcgcgact tgagctcaag ataacgcgcc agttgttcat
    25621 agcaacgcgc caggttgtcc tgcagcgggc ggaccgggaa cagcagatga ccaataagtg
    25681 tcaggacgtt gtaccagacg gcaccggcca gcagatacat cggctgctga taccagtgct
    25741 catacagtga tgttcccaac atagtgtaaa tggcgatcag caatgcaccg aaggcaattg
    25801 ttgcatagcg ttgacccaga ccgccgagca aaatgaagcc gctggtagag agcgttaagc
    25861 caatcgcaaa tagccagggc cagggaaaca gcaattctac tgaggccgag gcgataaaaa
    25921 agcagaacag cgtaatgatg aggttacgca aacgtcccgc cagtcggtca tcgagatcgg
    25981 tcagcgccgc tgccaccatc cccagcgtta gcggaatcgt cagttttaca tcacccagcc
    26041 accacggaaa cgctgtggtt ccacaaagcg caataaaaat acgcgcgtaa tacagccagg
    26101 cgctgttcca ggtatagcgt ttgagcaaag gacttagcat aaaggccata ttagtggatt
    26161 attcaaaacg acgacgtgca ttggcttcac gcgcagcttg cgctgtttct accgatacca
    26221 cgcgacgacc aaccggccat agtgcaatag cggcaatttt aaagttcgca atgccgacag
    26281 gaatgccaat gattgaaata cattgtgcga tgcccgttgc aatgtgcatc aggcataacc
    26341 accagccaaa gaaaatcagc cagaaaatat tcaataccgt accgccagta ttcagcagca
    26401 catttttgcc agccgggttc agttcatcga catgaatagc ttcattgcca taaggcacca
    26461 gagacagttt agtgatctcc cagcaggatc gtgtcagcgg taaggtaaaa atcagcataa
    26521 tactgaccag agtcgccaac agccagccca gagtggtggc aaatccgcca agcacaaagt
    26581 tcagaatgtt caaaacggta cgcataaaac ctcgctttac tgtggttttc agtaatgggc
    26641 ggcaattgta acgttttttt gggctggagc acgttttctc tgacggttac actgataaga
    26701 aataatttcg tgtggatcta cagagtcatg gaactgaaag cgacaacgct tggaaaacgt
    26761 ctggcacagc acccttacga tcgggcggtg atcctcaatg ccgggattaa agtctccggc
    26821 gatcgccacg aataccttat tcctttcaat caattactgg cgattcactg taagcgcggt
    26881 ctggtatggg gcgagctgga atttgtactg ccggacgaaa aagtggtgcg tctgcacggc
    26941 accgaatggg gcgagacgca gcgtttttac catcatcttg atgctcactg gcggcggtgg
    27001 agtggcgaga tgagcgaaat tgcgtctggt gttttacgcc agcaactgga tttgattgcc
    27061 acgcgcactg gagaaaataa atggctgacg cgtgagcaaa cctctggcgt gcagcaacaa
    27121 atccgccagg ctttgtcggc gttgccgttg ccggttaacc gactggaaga attcgataac
    27181 tgccgtgagg cgtggcgtaa atgtcaggcc tggttgaaag atattgaaag cgctcggttg
    27241 cagcataacc aggcgtatac cgaagccatg cttaccgagt atgcggattt tttccgccag
    27301 gtcgagtctt caccgctgaa tccggcgcag gcccgggcag tcgttaatgg cgagcattct
    27361 ctgttagtgc tggcaggtgc aggaagcgga aaaacgtcgg tgctggtggc ccgtgcaggc
    27421 tggttgctgg cgcgtggtga agcgtcccct gagcaaattt tattgctggc gtttggtcgc
    27481 aaagccgctg aagagatgga cgagcggatt cgcgaacggc tacataccga agacattacc
    27541 gcacgcacgt ttcatgcgct ggcgctgcat attattcagc agggcagcaa aaaagttccg
    27601 atagtcagca aactggaaaa tgataccgct gcccgtcatg aactctttat tgctgagtgg
    27661 cgcaagcaat gcagcgaaaa gaaagcgcag gcgaagggct ggcggcaatg gctgacggaa
    27721 gaaatgcagt ggtcagtgcc agaaggtaac ttctgggatg atgaaaaatt acagcgtcgc
    27781 cttgcctctc gcctcgatcg ctgggtaagt ctgatgcgga tgcacggtgg tgcacaggca
    27841 gaaatgattg ccagtgcacc cgaagagatt cgcgatctgt tcagtaaacg tatcaagttg
    27901 atggccccgt tattaaaagc ctggaaaggt gcgctgaagg cagaaaacgc tgtcgatttt
    27961 tcgggcctta ttcatcaggc gattgtgatt ctggagaaag gtcgctttat cagcccgtgg
    28021 aagcatattc tggttgatga atttcaggat atctcgccgc agcgggcagc gttgttagcg
    28081 gcattacgca agcaaaacag tcagacgacg ttgttcgccg ttggtgatga ctggcaggcg
    28141 atttaccgat tcagcggtgc gcaaatgtcg ctcaccaccg ctttccatga aaactttggt
    28201 gaaggcgatc gctgtgattt agacacgact taccgtttta acagtcgtat cggtgaggtg
    28261 gcaaaccggt ttattcagca gaacccaggc cagttgaaaa agccgctaaa cagcttaacc
    28321 aatggagacg aaaaagccgt cacgttattg gatgagagtc aacttgacgc tttgctggat
    28381 aagctctctg gttatgccaa accggaagag cgcattctta tcctggcgcg ttaccatcac
    28441 atgaggcctg ccagcctgga aaaagcggca acacgctggc cgaagttgca aatcgacttt
    28501 atgaccattc atgccagcaa agggcaacag gcggattacg tcatcatcgt tggcttgcag
    28561 gaggggagtg atggttttcc ggctgcggcg cgggagtcga ttatggaaga ggcgctactg
    28621 ccaccggttg aggatttccc ggacgctgaa gaacggcggt taatgtacgt ggcgctgacc
    28681 cgggcacgcc atcgggtatg ggcactgttt aacaaagaga atccctctcc ctttgtggaa
    28741 atactgaaaa atctggatgt gccggtagcg agaaaaccgt aagaaacagg tggcgtttgc
    28801 cacctgtgca atattacttc agacggtccg cgagataacg ctgataatcg gggatcagaa
    28861 tatcgaccgc gtcgttgaaa tgcggcgact ggattatgaa gtctgccgtt gccacgttgg
    28921 tggcgaccgg aatgttccat accgtcgcca gacgcaacaa ggctttcacg tcaggatcgt
    28981 gcggcacggc atttagtggg tcccagaaga aaatcaatac atcaattttc ccttctgaga
    29041 tcaatgcgcc aacctgctgg tcacccccca ttgggccact caacatcgcg ttgacgttca
    29101 tgccggtcgc gcgggaaatt aagttaccgg tagtgcctgt tgcatacagt acgtgttgtt
    29161 ccagtaacgg ttgatgccgt tccacccagc tcatcagcat ttgtttgcag tgatcgtgtg
    29221 ccaccagcgc aatatgtttc cgcgcaggta aagtgcgagt cgtcagttcc ataatgtaca
    29281 tccgtagtta actttcctac agattactgt aagcacttat cgctgcaaga taaagaccga
    29341 aaaagcctgc gcacaggcac aaaaatctca ggaagatggt tgtttttccg cccattgtag
    29401 gaaagtattt cgcgtttgtg ggtcagccag tttaaaccaa tacttcagcc gttgttctgt
    29461 gagcacctga gactgcggtg gaatagcatc cagctcggaa acacccgaaa gcagcgtact
    29521 gtcatctgcc atcatcgtgg caaattgcgg tagtgaagcg cgtttcgcgg atttgttata
    29581 gcgttcaacc tcgacctcat aatcaatcgt ttttgccgtt gaggtaatgg cgagaatatc
    29641 cagctttacc ggaatcgtcg tcgcatcgcc atccaacaat tcaaggcgcg gcgcggcatc
    29701 aaaatggtta gcttcccgct cattctccag gcgaggcagg cgaaaattga cctggttgat
    29761 gagctgggta ttaaaactga ccaccagcgg tggggagata tacagccgtt cttcactatt
    29821 ggaaagatga atcgtcttct caacgcgaaa cactaactga tgcggtccat tatccagttc
    29881 aatgctgtcc gcaccgcgca gcagagagct ggaaaccttc ttgccgtcga gtaccagaag
    29941 atcgacatcg gttgaaagcc gcagcgtggt ggcaaaaacg gataccggca aacataatgc
    30001 aatcaaggtg gtcacgatgc cggttttcat aggtagctcc tgtcaaaaga ccgcccgaat
    30061 aataatgtgt caaaattttt ctttaatcat acttactaac atatagacat atttcccgct
    30121 tttgctctca ttcattcgta ttagctgcat ggttggcatg tcggctttgt cgtacacttt
    30181 gttaaacagt ctggaggaaa taatgaaaga aaccgatatt gcaggcattt taacgtccac
    30241 tcacaccatt gcgctggtgg gggcaagcga caaacccgat cgtcccagct atcgcgtgat
    30301 gaaatatcta ctcgaccagg ggtatcacgt cattccggtt tcgccaaaag ttgccggcaa
    30361 aacgctgctg ggccagcagg ggtatggcac gctggcagac gttccggaaa aggtcgatat
    30421 ggtcgatgtt ttccgtaatt cagaagcggc gtggggcgtg gcacaggaag ctattgccat
    30481 tggcgcgaaa accctctgga tgcaactggg ggtgattaat gagcaagcgg cagtactggc
    30541 acgggatgcc gggttgaatg tggtgatgga tcgctgcccg gctattgaga tccctcgcct
    30601 gggcctggcc aaataaaaaa tccccggaag gcaaaaacct tccggggatt cgttcaggga
    30661 tcagttacgc agacgcgggg cctggagttg tttgcggatg gtctgcgcca gctcatccat
    30721 ggatggctgt tcaggatgtt catcctgcaa ttcgctactt agctgggctt cagccaggta
    30781 ggtatgaacc ggtaggccgt tatcgtcctc catcactacg tgataccacg gagcggcgcg
    30841 aagctcgtca tttaccgcca gctcatcagg cgacggttca gagagcgaat aaaccgggtc
    30901 gatatccacg accactccga gataacctaa cagggaatgg cggacctgct ggccgatacc
    30961 gaatttgctg gcaatcatag tcccctcccg ggaaatcttt acctttgata taagggtaaa
    31021 attccacatt tcaagttaca tgacgcgaca ggcaaaccct ttcagatata gcccttccgg
    31081 ataggtagcg atcaccggat gatcggctgc ctgacggaac tgctctataa attgtacatc
    31141 acggccggca tcaattgcgg catccgcgat gattttctga aataaatcgc tggtcatcag
    31201 accggaacag gagaaagtca ggagaatacc gccttcattc agcaactgaa tcgccagcat
    31261 gttgatatct ttatagccac gacacgcgcc catcaactgg cttttattct caacaaactt
    31321 cggcgggtcc atcacgataa cgtcaaattt ttcaccgcga tcgcgataag tacgcagcaa
    31381 tttaaagaca tcatcacgga caaactcagc cttgctcaga tccagtttgt tcagctcaac
    31441 gttctgccgt gcaatatcca gcgcttcctg ggaggtatca acgctgacaa cctggctgca
    31501 accgcccatc agtgccgata cggcgaaacc accggtatag gagaaacagt tcagcacacg
    31561 tttattttca acgtagcggc gggtagccag gcggatgtct cgctggtcca ggtagtagcc
    31621 cgttttgtgt ccgtgctgaa tatccaccag cagcttcatg ccgtgttctt caatcggcag
    31681 cagggcaggc ggcaactcgc cggtgacgag gccctgggtc agctccatcc cttctttttt
    31741 acgtaccgca acatcgctgc gatcgtaaat cgcacattcc gggtacagcg tttgcagggc
    31801 actaattaat gccgcgcgct gatattctgc cccagcactc agcagttgca gcaccagaaa
    31861 attaccgaaa cgatcgatag taatacccgg caggccatca gattctccgg cgattaaacg
    31921 atagctgtcg aggccatctt tttgcgccag ccagtcacgc catttttgtg cttgttgcaa
    31981 acggcgggaa aaaaaagcaa tgtcgataga ctcagacggg tcaaacgtcc agacgcgcgc
    32041 ccggatttgc gaagctggcg aataagcgcc gcgtgctaac cattttccct gatgatcaac
    32101 aatatcgatg gtttcaccga ggctggcttt accttccatg cgggcaacgg ccccggaaaa
    32161 gacccacgga tggcgacgaa gtaatgattt ttcgcgccct ttggctaaca ctaaacgtac
    32221 actcataatt tacttttctg tcgatgcaaa gaaatgggcg tcattgtccg gagttttcca
    32281 tggatttgca acgcgtccat taaggataag gagagcagca atgtcgaaag tctgcataat
    32341 tgcctgggtt tacgggcgag ttcagggcgt aggatttcgc tacaccacac agtacgaagc
    32401 gaaaagactg ggtttaaccg ggtacgccaa aaatcttgac gatggcagcg tggaagtggt
    32461 tgcctgcggt gaagaagggc aggtggaaaa attaatgcag tggttaaaga gtggcggccc
    32521 gcgttctgcg cgtgtggaac gggtgcttat cgagccgcat catccctcgg gggaattagc
    32581 cgattttcgt atccattaaa tacattttac cggttttggc aggccagcaa ttttggtggc
    32641 ttgcttagcc ggacctttcg gaaacagtcg atataaatag cggctgttac ctttttcttc
    32701 gccaaattta ttcgccatcg cttttaccag catacgaatc gccggagaag tattgaattc
    32761 cagatagaaa tcacgcacaa aacgcaccac ttcccagtgt tctggcgaca gcgaaatccc
    32821 ttcgttctct gcaatcacca cagccagcgg ctcactccac tggctgcttt ctttgagata
    32881 gccttcggta tccgtttcta tctctttacc ttcgaagatc agcataatta ttactacctt
    32941 aatcagactg gcggcagtgt aacaaaaaac aaagccccgc ataaagcgag gctatgaaag
    33001 tgttagcggg tgagattaat cgcggctagc gaagcccaga atgctcagca ggctgacgaa
    33061 gatgttgtac agcgaaacat acaggctaac cgtggcacga atatagttcg tctcaccgcc
    33121 atgaatgatg ttgctggttt caaacaagat agcgccagag gagatcagaa tgaagaccgc
    33181 gctgatcgcc agatgcagag caggcagctg caggaagata ttcgcaacca taccaatcag
    33241 caccaccaca atacccgcca tcagcatacc gccgaggaac gacatatctt tgcgggtggt
    33301 cagcacatat gcagagcagc agaagaacac taacgccgtt ccgcccagtg ccatagcgat
    33361 tacgtcaccc attccggcag acagataggt gttcagaata ggtccgagga tataacccag
    33421 aaaaccggta aaggcgaatg cggagataat cccggtcggc ttattcgccg ttttataggt
    33481 caggaacatc aaaccataca tacccaccag cgtcagaatc agacccggag atggcagcat
    33541 cagcaccgta ctggcagttg cggtaatcgc cgaaaaggcc agcgtcaggc tcagcagaaa
    33601 ataggtatta cgcagcacct tatgggtgct aagcagtgat gtacggtcat gtgaagaact
    33661 aacaatacga tccattgagt cactctctat gacagatgta attaattaag cagcataatg
    33721 ataatgcgta agggcaccca gaagttttac ccatctttac gcatttgatc tggaacaggt
    33781 ttaacagcgg attatcaggt cattaagcaa atataacgcc ctgagaattt cgacaggcaa
    33841 aagaaaaagg ggttagcatt tagctaaccc cttatcttat ttggcggaag cgcagagatt
    33901 cgaactctgg aaccctttcg ggtcgccggt tttcaagacc ggtgccttca accgctcggc
    33961 cacacttccg gaatgacgcg cactataaac atcccgatgc ggcgtgtaaa cccctaattt
    34021 gtttgtttgc ctgaaaaaca gccaaaagtg cattgatagc gtgaaataac agcagattga
    34081 tcatttcatc accatgaatt ccttctcttt tactcgttta gcaaccggct aaacatcccc
    34141 accgcccggc aaaaagaaaa ataggtccat ttttatcgct aaaagataaa tccacacagt
    34201 ttgtattgtt ttgtgcaaaa gtttcactac gctttattaa caatactttc tggcgacgtg
    34261 tgccagtgca gaaggatgag ctttcgtttt cagcatctca cgtgaagcga tggtttgcct
    34321 tgctacaggg acgtcgcttg ccgaccataa gcgcccggtg tcctgccggt gttgctagga
    34381 gaagagacgt gcgatatgaa taacgaggaa acattttacc aggccatgcg gcgtcagggc
    34441 gttacccggc gcagctttct caaatattgt agtctggctg ccacgtcgct gggattaggc
    34501 gcgggaatgg caccaaagat tgcctgggcg ctggagaaca aaccccgcat tccggtggta
    34561 tggatccacg gtctggaatg cacctgctgt accgaatctt ttatccgctc cgctcaccca
    34621 ctggcgaagg acgtcatcct ttccctgatt tccctcgatt atgacgatac tttgatggct
    34681 gccgccggaa cccaggcgga agaagtcttc gaagacatca tcacgcaata caatggcaaa
    34741 tatatcctcg cagtagaagg taatccgccg ctgggcgagc aggggatgtt ctgtatcagc
    34801 agcggtcgac cgtttattga gaaactcaaa cgtgccgctg ccggagccag cgcgattatc
    34861 gcctggggaa cctgcgcgtc ctggggctgc gtgcaggccg cgcggcccaa tccgacgcag
    34921 gcaacgccta tcgacaaagt catcaccgac aaacccatta tcaaagtacc tggctgcccg
    34981 ccgatcccgg atgtgatgag cgccatcatt acttacatgg tgacctttga tcgcttgcca
    35041 gatgtcgaca gaatgggccg tccactgatg ttttatggtc agcgtatcca tgataaatgc
    35101 tatcgccgcg ctcacttcga cgccggagag ttcgtccaga gttgggatga tgacgctgcc
    35161 cgcaaaggtt actgcctgta caaaatgggc tgcaaagggc ctaccaccta taacgcctgt
    35221 tcctccacac gctggaatga tggcgtttct tttccaatcc agtctggtca cggctgcctg
    35281 ggctgtgcgg aaaatggttt ctgggatcgc ggttcgttct acagccgcgt ggtcgatatt
    35341 ccgcaaatgg gtactcattc cactgccgat accgtcggtt taaccgcgct tggcgtggtg
    35401 gcagcggctg ttggtgtgca cgcagtcgcc agcgccgttg accagcgcag acgtcataac
    35461 cagcaaccta cagaaaccga acatcagcca ggcaatgagg ataaacaggc atgagcactc
    35521 agtacgaaac tcagggatac accatcaata atgccggacg ccgcctggtg gtcgacccga
    35581 ttacgcgcat cgaaggccac atgcgctgcg aagtgaatat taacgatcag aatgtgatca
    35641 ccaatgccgt ctcctgcggc accatgtttc gcgggctgga gatcattctg caagggcgcg
    35701 acccgcgcga tgcgtgggcg ttcgttgaac gtatctgcgg cgtctgtact ggcgtacacg
    35761 ccctggcttc ggtttacgcc atcgaagatg ctatcggtat taaagtgccg gacaacgcca
    35821 atatcatccg caacattatg ctggcaacgc tctggtgcca cgatcatctg gtgcacttct
    35881 atcagcttgc cgggatggac tggatcgatg tgttagatgc gctgaaagcc gacccgcgga
    35941 aaacctccga actggcgcaa agtctctcct cttggccgaa atcatccccg ggctatttct
    36001 tcgacgtaca aaaccgcctg aaaaaattcg ttgaaggcgg gcagttgggg atcttccgca
    36061 atggctactg ggggcacccg cagtacaagt tgccgccaga agccaacctg atgggctttg
    36121 cccactacct cgaagctctc gatttccagc gtgaaattgt gaaaatccac gcggtctttg
    36181 gcggtaaaaa cccgcatcca aactggattg tcggcgggat gccttgcgcc atcaacattg
    36241 acgaaagcgg cgcggtcggg gcagtcaata tggaacgcct gaacctggtg cagtcaatta
    36301 tcacccgcac ggcggacttc attaacaacg tgatgatccc cgacgcctta gccatcggtc
    36361 agttcaacaa accgtggagc gaaatcggca ctggtctttc tgataaatgc gttctcagct
    36421 acggcgcatt cccggatatt gccaacgact ttggcgagaa aagtctgctg atgcctggcg
    36481 gcgcggtgat taacggcgac ttcaacaatg tgctgccagt ggatttggtt gatccgcagc
    36541 aggtgcagga gtttgtcgac cacgcctggt atcgatatcc caacgatcag gtcgggcgtc
    36601 atccgttcga tggcatcacc gacccgtggt acaaccccgg cgatgtcaaa ggcagcgata
    36661 ccaacattca gcagctgaat gaacaggaac gctactcgtg gatcaaagcg ccacgctggc
    36721 gcggtaacgc gatggaagtg gggccgctgg cgcgcacgtt aatcgcttat cacaaaggcg
    36781 atgctgcgac cgttgagtcg gtcgatcgca tgatgtcggc gttgaacctg ccgctttccg
    36841 gtatccagtc aacgttaggc cgcattttgt gccgcgcgca cgaagcgcag tgggccgcag
    36901 gtaagttgca gtatttcttc gacaagctga tgaccaacct gaaaaacggc aatctcgcca
    36961 ctgcttccac ggaaaaatgg gaacctgcaa cctggccgac agagtgccgt ggtgtcggtt
    37021 ttaccgaagc gccgcgcggg gcgttaggcc actgggccgc cattcgcgat ggcaagattg
    37081 atctctacca gtgcgtggtg ccgaccacct ggaacgccag cccgcgcgat cccaaagggc
    37141 agattggcgc ttatgaagcg gcgctgatga acaccaaaat ggcgatcccc gagcaaccgc
    37201 tggagatcct gcgtactctg cacagctttg acccgtgcct cgcctgttca acacacgtgc
    37261 tgggcgacga cggtagcgag ctgatctccg tgcaggtgcg ttaacagcga aggagaatca
    37321 tcatgcaaca gaaaagcgac aacgttgtca gccactatgt ctttgaagcg ccagtccgca
    37381 tctggcactg gttgacggtg ttatgcatgg cggtgttgat ggtcaccgga tactttatcg
    37441 gcaagccgct accttccgtc agcggcgagg cgacgtatct gttctatatg ggctacatca
    37501 ggttaattca cttcagcgcc gggatggttt ttaccgtggt tttgctgatg cggatctact
    37561 gggcttttgt tggcaatcga tactcccgcg agctgtttat cgtgccggta tggcgtaaaa
    37621 gctggtggca gggcgtgtgg tatgaaatcc gctggtatct gtttctggca aaacgtccga
    37681 gtgccgatat aggccataat cccatcgccc aggcggcgat gttcggctat ttcctgatgt
    37741 cggtctttat gatcatcact ggttttgcgc tgtacagcga acacagccag tacgctattt
    37801 ttgcgccgtt ccgttatgtg gtggaatttt tctactggac gggtggcaac tcaatggaca
    37861 ttcacagctg gcatcggctg gggatgtggc tgattggcgc gtttgtgatc ggtcatgtct
    37921 acatggcgct gcgtgaagac atcatgtccg acgacacggt gatctccacc atggtcaacg
    37981 gctaccgtag ccacaaattt ggcaaaataa gtaacaagga gcgttcatga gcgagcaacg
    38041 cgtggtggtc atggggctgg gcaacctgct gtgggccgat gaaggcttcg gcgtgcgggt
    38101 ggcggaacgg ctgtatgccc attaccactg gcccgagtat gtggagattg tcgatggcgg
    38161 tactcaggga ctgaacttgc tggggtatgt cgaaagcgcc agccatctgt tgattctcga
    38221 tgccattgac tacgggctgg aacctggaac gctgcgaacc tatgccggag aacgcattcc
    38281 ggcttatctc agcgcgaaga aaatgagcct gcatcagaac agtttctccg aagtgttggc
    38341 gctggcggat atccgcggac atctgccagc acatattgcc ctcgtcggtc tgcaacccgc
    38401 aatgctcgac gactacggcg gtagcctgag cgaactggca cgggagcaac tgcccgctgc
    38461 ggaacaggcg gcgctggcgc agctggctgc gtggggaatt gtgccgcaac cggctaatga
    38521 atcgcgctgt ctcaattatg actgtctgtc gatggaaaat tacgaaggcg ttcgcttgcg
    38581 ccagtaccgg atgacacagg aggagcaggg atgagcaacg acacgccatt tgatgcgttg
    38641 tggcaacgaa tgctggcgcg cggctggacg ccagtcagtg aatcccgtct tgacgactgg
    38701 cttacgcaag cgccagacgg cgtggtgtta ttaagcagtg acccgaaacg cacgccagag
    38761 gtcagcgata atccggtaat gattggcgaa ttactgcgcg agtttcccga ctatacatgg
    38821 caggtggcga ttgctgacct tgagcagagc gaagccatcg gcgatcgctt tggcgtcttt
    38881 cgctttcctg ccactttagt gtttaccggc ggaaactatc gcggcgtgct gaatggtatt
    38941 cacccgtggg cggaactgat aaacctgatg cgcgggcttg tcgaaccgca gcaggagcgt
    39001 gcctcatgag cgaaactttt ttccatctgc tggggccagg aacgcaaccg aacgatgaca
    39061 gtttcagcat gaatccactg ccgatcacct gtcaggtgaa tgatgaaccg agtatggcgg
    39121 ccctggagca atgtgctcac agcccgcagg tgattgcgct gttaaacgag ttacaacatc
    39181 aactaagcga acgccaaccg ccgttgggcg aggtgctggc agtcgatctg ttaaatctca
    39241 acgccgacga tcgtcacttt atcaatacgc ttctcgggga aggggaagtg tcagtgcgca
    39301 ttcagcaggc tgacgacagt gaaagtgaaa tacaggaggc gatcttctgc ggattatggc
    39361 gggtgcgcag acgtcgcggc gaaaagttgc tggaggacaa actggaggct ggctgcgcgc
    39421 cgctggcgtt gtggcaggcg gtaacgcaaa atctcttgcc gacagattcg ctgttaccgc
    39481 cgcccattga tggcctgatg aatggcctac cgttggcgca tgagttactg gcacatgtac
    39541 gtaaccccga cgcgcagccg cacagcatta atctgacgca attacccatc agcgaggctg
    39601 atcggctttt tctctcacgt ctctgtgggc cgggaaatat tcagattcgt accattggct
    39661 atggcgagag ctatatcaac gccacggggt tacgccatgt ctggcattta cgctgtacgg
    39721 acaccttaaa aggcccgtta ctggaaagtt atgaaatctg cccaataccg gaagtggtgc
    39781 tggcagcgcc agaagatttg gtcgactctg cgcagcggct tagcgaggta tgtcagtggc
    39841 tggcggaagc tgcaccgacg taaaatgacg gtaagtatcg ctttcagtct tatgaatatc
    39901 gcaatcggcg aatacctctg gtcgtagagt ttcaggataa agagggagat ctaccattat
    39961 cgggttattt ttctctcttc gcctacagga gtgcgcatgt gggatgtcat tgatttatcg
    40021 cgctggcagt ttgctctgac cgcgctgtat cactttttat ttgtacccct taccctgggg
    40081 ctgatttttt tgctggctat catggaaacc atttacgtgg tcaccggcaa aacaatctac
    40141 cgcgatatga cgcgcttctg gggtaagctc ttcggtatca attttgctct tggcgtggct
    40201 accggcctga ccatggagtt tcagtttggt actaactggt cattctattc caactatgtg
    40261 ggcgatattt ttggcgcacc actggcgatg gaagcattaa tggccttctt cctcgaatcc
    40321 acctttgtcg ggctgttctt cttcggctgg cagcggctga ataaatacca gcacctgctg
    40381 gtgacgtggc tggtggcgtt cggttcaaat ctctctgcat tgtggatatt gaatgccaac
    40441 ggttggatgc aatacccgac cggtgcgcat tttgatatcg acaccctgcg tatggagatg
    40501 accagcttca gcgagctggt ctttaacccg gtcagccagg tgaaatttgt gcacaccgta
    40561 atggcgggct acgtgaccgg ggccatgttt attatggcga tcagcgcctg gtatttactg
    40621 cgcggacggg agcgcgatgt cgcattacgt tcgtttgcca tcggttccgt cttcggtact
    40681 ctggcgatta tcggtaccct gcaactcgga gacagttctg cgtatgaagt cgcgcaagta
    40741 caaccggtaa aactggcggc gatggaaggg gagtggcaaa cggaacctgc acctgcaccg
    40801 ttccatgtgg ttgcctggcc ggaacaggat caagagcgta acgcctttgc catcaaaatt
    40861 cccgcgctgc tagggatcct cgccactcac tcattagata aacccgtgcc gggtctgaag
    40921 aatttgatgg ctgaaaccta cccacgcttg caacgcggac gtatggcctg gctgttaatg
    40981 caggaaatat cgcaaggcaa tcgtgagccg catgtgttgc aggcattccg ggaactggaa
    41041 ggtgacctgg gctacggcat gttgctctcc cgctatgcgc cggatatgaa tcatgtcaca
    41101 gccgcacagt accaggcggc gatgcgtggc gcgatacctc aggttgcgcc ggtattctgg
    41161 agtttccgca tcatggtggg ctgtggttcc ctgctgctac tggtgatgct gattgcgctt
    41221 gtccaggcgc tgcgtggcaa aatcgaccag catcgctggg tgctgaaaat ggcgctctgg
    41281 agtttgccgt tgccgtggat tgcgattgaa gccgggtggt ttatgaccga gtttggtcgt
    41341 cagccgtggg cgatacagga catcttaccg acatactccg cgcactccgc tttaaccaca
    41401 ggacaactgg ctttctcact gatcatgatc gtagggcttt acaccctgtt cttaatcgcc
    41461 gaagtctacc tgatgcagaa atatgcccgt ctggggccga gcgcgatgca gagtgaacaa
    41521 ccgacgcagc aacaggggta gaggagaaaa tcatgtttga ttatgaaaca ttgcgcttca
    41581 tctggtggct gctgattggc gtgatcctgg tggtctttat gatctccgac ggatttgaca
    41641 tggggatcgg ctgtctgctg ccgctggtgg cgcgtaatga tgatgaacgc cggatagtga
    41701 taaacagcgt tggtgcacac tgggaaggca accaggtctg gttgatcctc gctggtgggg
    41761 cattatttgc cgcctggccc agagtgtatg cagcggcgtt ttccggcttt tatgtggcga
    41821 tgatcctggt gctgtgctca ctgttcttcc gcccgctggc ctttgattat cgcggaaaaa
    41881 tcgccgatgc acgctggcgt aaaatgtggg acgccggtct ggtcatcggc agtctggtgc
    41941 cgccggtagt cttcggtatc gccttcggca acttgttgct cggcgtgccg tttgccttca
    42001 caccgcaatt acgcgtggag tatcttggca gcttctggca actgctgacg ccattccctt
    42061 tattgtgcgg attgctcagc cttgggatgg tgattttgca aggtggcgtc tggttacaac
    42121 tgaaaactgt tggtgtgatt catctgcgtt cacagctggc caccaaacgc gctgcactgt
    42181 tggtgatgct gtgctttttg ctggcgggtt actggctgtg ggtcggtatt gatggctttg
    42241 tactgctcgc ccaggatgct aacggtcctt ccaatccgtt aatgaaactg gtggcagtgc
    42301 tacctggtgc ctggatgaat aatttcgtcg aatcgcccgt tttgtggatc ttcccgctgc
    42361 tggggttctt ctgcccattg ctgacggtga tggcgattta tcgtggtcgc ccgggttggg
    42421 gatttttaat ggcgtcattg atgcaatttg gcgtgatttt cacggcaggc atcacgctgt
    42481 tcccctttgt catgccgtca agcgtgagtc caatctccag cctgacgttg tgggacagca
    42541 cttccagtca gctgacgctg agcattatgt tggtaatcgt gctgatattt ttgcccattg
    42601 tgttgctcta cactctctgg agctactaca aaatgtgggg acgcatgaca acagaaactc
    42661 tccgccgtaa cgaaaacgag ttgtactaag gagcaggaac aatgtggtat ttactttggt
    42721 tcgtcggcat tttgttgatg tgttcgctct ccacccttgt gttggtatgg ctggacccgc
    42781 gtctgaaaag ttaacgaacg taggcctgat gcggcgcatt agcatcgcat caggcaatca
    42841 ataatgtcag atatgaaaag cggaaacata tcgatgaaag cgatcttaat cccattttta
    42901 tctcttctga ttccgttaac cccgcaatct gcattcgctc agagtgagcc ggagctgaag
    42961 ctggaaagtg tggtgattgt cagtcgtcat ggtgtgcgtg ctccaaccaa ggccacgcaa
    43021 ctgatgcagg atgtcacccc agacgcatgg ccaacctggc cggtaaaact gggttggctg
    43081 acaccgcgcg gtggtgagct aatcgcctat ctcggacatt accaacgcca gcgtctggta
    43141 gccgacggat tgctggcgaa aaagggctgc ccgcagtctg gtcaggtcgc gattattgct
    43201 gatgtcgacg agcgtacccg taaaacaggc gaagccttcg ccgccgggct ggcacctgac
    43261 tgtgcaataa ccgtacatac ccaggcagat acgtccagtc ccgatccgtt atttaatcct
    43321 ctaaaaactg gcgtttgcca actggataat gcgaacgtga ctgacgcgat cctcagcagg
    43381 gcaggagggt caattgctga ctttaccggg catcggcaaa cggcgtttcg cgaactggaa
    43441 cgggtgctta attttccgca atcaaacttg tgccttaaac gtgagaaaca ggacgaaagc
    43501 tgttcattaa cgcaggcatt accatcggaa ctcaaggtga gcgccgacaa tgtctcatta
    43561 accggtgcgg taagcctcgc atcaatgctg acggagatat ttctcctgca acaagcacag
    43621 ggaatgccgg agccggggtg gggaaggatc accgattcac accagtggaa caccttgcta
    43681 agtttgcata acgcgcaatt ttatttgcta caacgcacgc cagaggttgc ccgcagccgc
    43741 gccaccccgt tattagattt gatcaagaca gcgttgacgc cccatccacc gcaaaaacag
    43801 gcgtatggtg tgacattacc cacttcagtg ctgtttatcg ccggacacga tactaatctg
    43861 gcaaatctcg gcggcgcact ggagctcaac tggacgcttc ccggtcagcc ggataacacg
    43921 ccgccaggtg gtgaactggt gtttgaacgc tggcgtcggc taagcgataa cagccagtgg
    43981 attcaggttt cgctggtctt ccagacttta cagcagatgc gtgataaaac gccgctgtca
    44041 ttaaatacgc cgcccggaga ggtgaaactg accctggcag gatgtgaaga gcgaaatgcg
    44101 cagggcatgt gttcgttggc aggttttacg caaatcgtga atgaagcacg cataccggcg
    44161 tgcagtttgt aatgcataaa aaagagcatt cagttacctg aatgctctga ggctgatgac
    44221 aaacgaagaa ctgtctaatg cgtagaccgg aaaaggcgtt cacgccgcat ccggccactt
    44281 tcagttttac tctttctcgg agtaactata accgtaatag ttatagccgt aactgtaagc
    44341 ggtgctggcg cgtttaatca caccattgag gatagcgcct ttaatattga cgcctgcctg
    44401 ttccagacgc tgcattgaca aactcacctc tttggcggtg ttcaagccaa aacgcgcaac
    44461 cagcaggctg gtgccaacag aacgccccac gaccgcggca tcactcaccg ccagcatcgg
    44521 cggcgtatcg acaatcacca gatcgtaatg gtcgttcgcc cattccagta attgacgcat
    44581 ccgatcgcgc atcagcagtt cagacgggtt aggtggcacc tgaccgcgag taatcacatc
    44641 aaagcctcct ttgccaaaat gctggatcac tttgttgagc tcatctttac ctgccagata
    44701 ttccgacaag ccatgttcat tactcacggt aaacaggtta tgcgaataac cacggcgtaa
    44761 gtcggcatca ataaataaca ctttttgatc ggactgggcg atcaccgctg ccagagttga
    44821 actgacaaac gttttaccac tgtctggcgt cgcaccggtg atcatcagaa tgttattctc
    44881 cgtctccatc atagcgaaat gcagactggt tcgtagcgca cgtacggctt ccacagcaga
    44941 atccgccggg ttatccaccg ccaggaaggg gatattttta gtacgatggc gctgctgatt
    45001 agaaaataaa tttttcttac gcagacgggt gcgtttatcc agccactcgg acattgggat
    45061 agtggcataa acgctgatgc cgtgctcttc cagttgttcc ggggcttcta caccacgacg
    45121 caacatcgca cgcgccagca cggcacccac agaaataaac aggccaagaa taaaaccaag
    45181 caccacattc aacgcttttt tcggtttcac tggctgcggc tgagtgactg ccgggtcgat
    45241 aatccgcacg ttaccaatgg cactggattt cgaaatactc aactcctgct ggcggttaag
    45301 taattgcaga tataccgcac ggcccgcttc tacgtcacga cttaaacgca acacttcctg
    45361 ttgggtggaa ggcattgccg atacccgctt attcaggcgt ttgcgttctt gctccagcgt
    45421 ctggcgtttt tccagcagcg cacgataagt tgggtgatct ttcttataca gctgggagat
    45481 ctctgcctcg cggaaagtca gctcattgag ttgattatca acgttcacaa tctgctcaag
    45541 aacggctttg gcttccaggt taaggtcaac cgaatcgcgc tgctggcgat aaacgttgag
    45601 tttttcttcc gcctggtcca gctcgctgcg cacttcaggc aactggcgct gtaagaattc
    45661 aaggctttgt gaatcctgcg ccgcctggcg agcgatattc tgttgcaaat agttgttagc
    45721 gatgctgttc agaatacgag taatcaactg gggatcatca ccggtcatgg taagttccag
    45781 catcccactt tctttactgc gttcgctaac ggtaaaggtt tcctgcaacg cgttaatcgc
    45841 ttccagttcg gtacgctggc tcaggacaaa ctgtgttcct ggtttggcct taatatccgc
    45901 gatagtcagc gcaacgccat ctttttccag acgctggccg accataccat tgacggtgaa
    45961 ctcttcacct tccagcgtat agtggccgtt ttccccaacc gtgagtgtca gttgctgatc
    46021 ctgaccattc agttgtggaa tatgcatcca gctgatcgcc agctcacctg gtttctcttt
    46081 ggttaatctc gcccagccgc gacccacaat cggaaaatac ttctgctcaa ccatgtcgcg
    46141 cagattcagt tcagcaatgg ttttaccgag aatcatgcgc gattgcagca gttggatctc
    46201 cggtgcagac tcgggcgatg agttagggat catatcgctc aggccactga gaatggcgtt
    46261 gccctgtttc tgctcaacct ggaccagagt atctgcctga taaattggcg tgcttaacag
    46321 cgagtaagcg acagcgatca gcgtgaataa cgcggtcacg ctgataataa acttacggtg
    46381 atcccataac tcgccgacca gacgaagcag atcgatctca ttttcctgag tgctgcctgg
    46441 tggcgtattc atatttttag ttgtcatcgt attttataaa ctcaaattac cggctgaggc
    46501 gcttcgccca ttcctgactg gcgcgctcca acataccgta gacatgttca aatgcgtcct
    46561 gacttttacg atagggatcc gggatctctt tctgttccag ccattgccca aacagcattg
    46621 tcttcccgcg gacctcggga gcgattgcgg taacctgagc aatatgttcc gactccattg
    46681 ccagaatcag atcgtaattt cgtgccatct cagcagtgag cttacgtccg gcatgtcctt
    46741 caagagatac gccatgatta gcggcaacat ctgccgctgt cgcatctgca gggtgtttta
    46801 ccagaccatg aacacctgcc gatttgactt ttacgcccgg cagacgttta cgcagcaagc
    46861 gctcgccaat gggcgaacgg caaatgttgc cggtacaaac caccaggatt gagttaaatt
    46921 ttagttgggc catgtttgaa tccaccgtga agtctctgtc agatcatgca ccccggaaat
    46981 cgtcggtacc agcagagaaa tcacacggtt ccaacgagcc agcggcgcgg tagtgacata
    47041 aacaatgtcg taaggttcaa gctggaattc cgttccgagt atcattgccg atgcgtcctt
    47101 cgcattcagc tgataaatat tggcgatttt gccattctgc ttgttttgcg ttgcacgaat
    47161 aacaaaaatc cccgtcgcat cagcgacatc ctgattcata ccctcagcgt tacccagcgc
    47221 ttccgccagg gtcataccgc tgcggtccat tttcagcgtg ctctgtttac ccacttcacc
    47281 catcacgaag actttcagat catcgttgcg cggaataaat aaaatatcgc ccgggtgcag
    47341 cagtttgttc tggcgcaagt cgccacgctg catcagcgca tagaggttca ctttggtctt
    47401 tacgccattc tgggtgagca ccacgttgcg ccagtctgca tctgcggtca gaccgcccgc
    47461 ggcgttaatc gcatccatga tggtcagagg aatattggta atcggctgct gacccgattt
    47521 gctgacttcg ccggtgacgt acgctttttg cgatcggaac gccgcgacgc tgacatcgac
    47581 ttgcgggctt tcaatgactg aatcgagacg cgctgtaatc tcgttgcgta cctgggtaag
    47641 tgttttcccg gccactttca ggcgaccaat ataggggtag aaaatcgcac catcggcgtt
    47701 aacccagttg ccggtatcac tggcgctacg gtattgccct gcgggggtgg ttagttccgg
    47761 gtgatcccag accgtaacca tcaacacatc accaatcccg atacgatatt catagtttgc
    47821 gatttgctgc tccagctccg ggttagcctg cgacataatc ggtttggcgc gcaactgatc
    47881 aatcaatcct ggcgtgacgg gatagatatt gaccattttg tccaggtcat actgattgtc
    47941 aggtagttca atgacatcct tgttactggt cgataaattc tgaccaggaa caagggtaca
    48001 tgctgtcaaa gacagaacag ccaatgtcag taccgaaaac ttaaaaatgt tctttttcat
    48061 tggtttatat ttatcagtca atgtaatgtt atgaattacg tcaggttatt gtccaaccgg
    48121 tacttctcgc tctgccgtca ttggatagag ctggtatttg cgtgacagca tttgtccacc
    48181 atcacgcgtc aatggtgtcc acgaaaccac cgcgcggttg cggttaggtc caatggtcat
    48241 caagtcgaat ggaatcgaga tataaaaacc tttactaaag ccgccttcgc cgtagtcatc
    48301 tttagatacg ttactgattg ccgcccatac ccctaccgcc acgccgctgt caaagcgttt
    48361 ggcgacgtcg atcgttgcgc ctttatcttt tgccagatat tgcccaacgc taagtttcat
    48421 cagtacgcca ttgagcgtcg gcgggttcca gtaagccgtc acgaaacctg ttggcgtgga
    48481 ataatcggtg aaacgcatca tgttgtccca gtcacgctgc ttcacgtagt taacgtccac
    48541 acccagcgcc cagctggcat ctagcgggcg ataaagcagc tcggaaccga caccggcata
    48601 catcgtttcc agataaccgc catacacctg gccatagaaa ccattgccta agtcagcaaa
    48661 atagttcgcc tgcaagttgt tgagataaac gtcattgcga acgtagtcac ggatatgcgt
    48721 gcgcacgcgg ggcagggtag agtccgcggg caacagcgaa gacttaaact tgtcgtagtt
    48781 gttgtaaata ttggtgaaaa taccgccatc aagcagcagg tggtcggtaa accagtagcg
    48841 ggcactggac atcagcccca gctggaacat atagaaatct tccgggccgc ccagcgactg
    48901 tgaaagtgtt gggttgaaag agtagctaaa gcgatcttca cgaatacggt agccccgacc
    48961 aaaggcagaa agatcttctg cttcaacacg ttgttgttgc agttgctctg attgaccagg
    49021 cgctgtacca gccagctgtt tgcgcaggct ggctacgtcg gtttcggtag tcaccatcgc
    49081 catatgctcg cgcttttgcg tcacgctaat cttctcaacg ccttgcggca ggttattcac
    49141 cagaatccgg ttggcacgat cgaccgcttc acgagagtca cggtatttgt attgctgacc
    49201 agacatatac agcgtcttat cgcgtagctg aatttctggc gcgtcaaagc ccgcgttata
    49261 cttcagggcg gtaagttggt ttgctaccgt ggtgtactgc aatccttcag attcaggcgc
    49321 aggttgatat gccggttttg gcgtatcgcg cagggcaggg cgcagatcgt tgaaattggt
    49381 ccgtaaagtg aaaccaaaca tcaacgtgtt accgcgttca taactcaggt tgagatctgc
    49441 ccagctggca gcgcgataaa ctgcaccgac gttgaaatgg cttgcctgag gcagtttgcc
    49501 agcgaaatca ttctggtaat tgttgccgtc gtattcgagt ttcagacgca ggggattcca
    49561 cggcgtttga tactcaatgc cgccaaagat ggaagccggg ccacgaaaga tatcgctaaa
    49621 gctgatatcg cccgcatcgt gagactctgc gcgatgacaa tatttatcgc tcacccggca
    49681 aaacgggttg gtaatattgc ccgcattacc ggcgtagccc catgccatcc cgagggtgaa
    49741 atcaaatggc cccgcttgct tgctggccac cagatactca ccgtcaaaca gacccgtacc
    49801 agcaatatca cgtttaccaa acgccacttg cggtagccag taaccttctt cccacaggcg
    49861 aagtttaaaa tcgaatgatt tgtctttgta tgactgatcg ccgctgaaat cttcccactg
    49921 gctatatttg cgtgtgcgca catccgtata acgaatggtg ccttccagcc atgggaacag
    49981 cgccacggag gtggagtaga accggtattg atcgttatcc cggtagttga cgctgaattc
    50041 accttccggt gcgatgcgcg catttggcat ctgcaacaat cctgtgccgc caaagtctga
    50101 ttgcgacgaa cccagcggat ccgggtaggt taatacttca gcatgacagg cactggagac
    50161 ggcaatggcc aggcagctta aaagataaga attcttcttc atcagtcagg aacacgttgc
    50221 gttaaaacag acacaatctg gtcatttaaa tcagcatatt tttcgggcag tacatgcgca
    50281 gaaaagccca accaaagctg gctgccaggc ggtggttcca catgtctttt attccacagg
    50341 gcaacagggg cgacgacagt ctcaccctca ggcgtaatca ccataacgtt attcttgtcc
    50401 gcgcctgcaa ggcgggggtg atcttgcaga taatcggtaa cgctacggcc tgcctgccag
    50461 ggcaattgcc cagccccaga aaccgcaccc agcagagtaa tagtcaccgg acgctgtacg
    50521 gtatagagcg tatagtcccc gaccaacggg ggattgctgt tctcatccac tcgtacaaaa
    50581 tcagggtcca gtttgaccgg taagcgacca gtgatattga gattgagtaa ctgctggcgc
    50641 acggatttaa tcgttgccgc cacatcgtcg tcagcttctg cttcccagga agcaagctgt
    50701 gccatgacat gttggtagtc tttaagcgct ttagctttcg ccgcagagtc ggtcagcaag
    50761 gcaccaggcc accagaggcg atcgcgcagt tgcggttgtg tcaccaactg gacaacattt
    50821 tccacggggc caacggaaag tgtctgttgt tcgccaggca gataaatagt caccgttcct
    50881 tgcgcaaagg catggggtgt cattacgtaa agtacgctgg caatgaaata cgactgtaat
    50941 ttattcatgg tgcgggttta agaaacgtca tttctacggg aataacccct gcgcctaaca
    51001 tttgccgact ttgacgaacc tggccagtag ccgaatcgac ccagaatgtg ttttgccact
    51061 gtttttctgg ccgcgtggaa gtcacctctt cctgccagac attacacagc acattgcgac
    51121 ccgcaatatt cagcgtctct aatccagcaa aggaaaaagt agaactcagg gtcgcagagc
    51181 gaaaatggtt gtcttcactc cagagaatat cgcgcgtcca gcggctgcca tcttttattg
    51241 ccagcgcgtt acgcagaggg tcctgcccgg aatttgtcac ttccagcaga ttattattaa
    51301 gtttgacggt ttttaaaaga cgtccattgt gggtaaccag catggcgtta tcctgggata
    51361 accatttgct ttgttcttgt tctatatatc ccagtaccac aaagattcgt tgcccttcat
    51421 taaggcgtaa atacatcgtg gaataaggca acgcctggat ctgctgatcc gctacggtga
    51481 tatcctgatt atcgaaaagg ctggcgcgaa atgtatcgac catactttgc tggctgtgcg
    51541 tacatcccgc aagaaatagt gcgaaaatcg ataaaataag agggcgcacc tttaatcctt
    51601 gaaaggttaa ttaaaaaaat gcaaatctct aaagcccggt acgaatatcg caaccgggta
    51661 tcatttaact ttaatttcga cgcgttaaag cagtattagc gtgtagtgga cgtggtggtt
    51721 gaggtgttat gagaaccgcc atcgccactg tcatttgctg caacaactgc caacgtcgcc
    51781 acaccaacag ccgttgccac accgacaact gtcgccgtgc cgacagcgcc agcagccacg
    51841 ccaccagtgg tagtagcagt cgtggttgcg ccggtagtgg tcgttgtggt gccagtagta
    51901 cccgtcgttg cctcggtccc gtttgttgct tcaggggcag caaacgcagc tggcgtcgtc
    51961 aacatgaagg ccataaggat tgcagaaagc ttgtgtttca taacttttcc tttattcatc
    52021 gcatggacaa tacgtacgct tgagatcagg attgtctact ttgagtataa catccttgtg
    52081 aaatctccct ttcctcatac cgcttgcact atatactgcg ggtaggaaag gcgcgcagag
    52141 ggaaatataa gattgttaac tatccccctc tggaaatacg acttgtgagc ataagataaa
    52201 tcttagcaaa atacaaatta aagataagga aattgggtga tgctgccaac ttactgattt
    52261 agtgtatgat ggtgtttttg aggtgctcca gtggcttctg tttctatcag ctgtccctcc
    52321 tgttcagcta ctgacggggt ggtgcgtaac ggcaaaagca ccgccggaca tcagcgctat
    52381 ctctgctctc actgccgtaa aacatggcaa ctgcagttca cttacaccgc ttctcaaccc
    52441 ggtacgcacc agaaaatcat tgatatggcc atgaatggcg ttggatgccg ggtaactgcc
    52501 cgcattatgg gcgttggcct caacacgatt ttacgtcact taaaaaactc aggccgcagt
    52561 cggtaacctc gcgcatacag ccgggcagtg acgtcatcgt ctgcgcggaa atggacgaac
    52621 agtggggcta tgtcggggct aaatcgcgcc agcgctggct gttttacgcg tatgacagtc
    52681 tccggaagac ggttgttgcg cacgtattcg gtgaacgcac tatggcgacg ctggggcgtc
    52741 ttatgagcct gctgtcaccc tttgacgtgg tgatatggat gacggatggc tggccgctgt
    52801 atgaatcccg cctgaaggga aagctgcacg taatcagcaa gcgatatacg cagcgaattg
    52861 agcggcataa cctgaatctg aggcagcacc tggcacggct gggacggaag tcgctgtcgt
    52921 tctcaaaatc ggtggagctg catgacaaag tcatcgggca ttatctgaac ataaaacact
    52981 atcaataagt tggagtcatt accaggaaat tgttacgaaa gctattaatt attgttggta
    53041 atatagtttc aagtggtact ataactggct gttaaatggc atttataact attaggtgca
    53101 gagatattcg cttaaatgga ttagtttctt tgtggaaaag taactgatat tataatgtat
    53161 ggtgttgact attgcactat tccaataaaa ccagtcagct ttaaacaagc agcgtcatat
    53221 taagagagat aaacatttgc cgctgttggt cctcgcaggc catttacgcg gcaaaattcc
    53281 acacgtaatc ctggtataag cacttctgcg tcgcggggag tgaatgcgga aatatggacc
    53341 tgaacttctt tacgaccgtc ggaggggata atgaatcctt tgccgctttt gcgatcaaag
    53401 gttttgacaa ttcctgtcat tttacgggac aaacaaattc cttactgaaa atactgcgct
    53461 gcactatacg gggttaataa aataaagcca gcgatattta agaccgccgg acggctaaaa
    53521 taaaatttgc ttaatctcaa ttatcatgcg ttaatagctg cgtcggtttg aaagacagac
    53581 agcatacaaa gtagtttact aaagcagttc tcattatcag gcattatccc cttcttttga
    53641 gtctctctcc tgaacactaa gtagtttctg tattaaagcc ctgtttgccg aaaggcccaa
    53701 aatgaaggaa gtaaaatatg tctaataaaa tgactggttt agtaaaatgg tttaacgcag
    53761 ataaaggttt tggctttatc actcctgatg atggcagcaa agacgttttc gtccatttca
    53821 ctgccatcca gagcaatgaa ttccgcacgc tgaacgaaaa tcagaaagtt gaattttcta
    53881 ttgagcaggg gcaacgtggc cccgcggcag cgaacgttgt tacgctctaa ggttgccgtt
    53941 attactcaac atctccattt ccgctgtcca tgttgtcatg gttcacagta ccgtacatcg
    54001 gcattcgatg tgacggagcg aaaccctttg ggcgctaagt gtattttttg taaatcgacg
    54061 atgatcacct ttgataacgt cgcgctgcaa atacgcacgg accatgcgcc gctggatttc
    54121 acaaaataat atcaggctcc ctcgtggagc cttttttata tctgccttat ttttcttcaa
    54181 cgctgtatgt atagtaagcg ataacctgtt gattattgaa tctttcgggg agatggctta
    54241 taacatttct tacctgacca gggtaccggg aaccaacacc ttactggcgt gttgctgtct
    54301 ttcaagacca gaagaggtta acagtgaata ttgaagagtt aaaaaaacaa gccgaaacgg
    54361 aaatcgccga ctttatcgcg caaaaaatcg ccgagctgaa caagaataca gggaaagaag
    54421 tctctgaaat tcgcttcacc gcacgagaaa aaatgaccgg gcttgaaagt tatgatgtca
    54481 aaatcaaaat aatgtgattt tgtgaacatc acccgtgcga ggtgatgttc cgcttgttgc
    54541 taatttagtg accaatcatt ggcgcttgtg gaattaagcg tcggtacaat tcatccggta
    54601 ccgggctttg ccatactccc gcatacattg cgtaaccaat caccgcaaac ataatcccca
    54661 gaaccagtag cgtcattaac cagccagaca acgcaaaggc ttttttattt gccgcaggtt
    54721 tttgcagtga aaaggtcaat gttgaggcta ccggacatga ctctacgcaa gtcatacagc
    54781 cggtacattc cactgttcgt acctgaatta atttatcgac cgggatccgt gatgggcaat
    54841 tttttgcgca tttgccacag tcgatacaac tttcggcatt gcgacgaatc ttaaacggcg
    54901 acaatagcga aaccacgccc atcagcgcgc catatgggca aagataacga caccaggcat
    54961 ggcgaataaa caggctggca atcagcaaaa cggtcacgct gattaatgtc gcggtcccca
    55021 tatgacgaaa gaaatcgagc attttaacgt ccatcaccac gctgtagggc gacaacataa
    55081 aatagtgaat cgcctgagcg ggcatcaata acgcgatata gagaaaaaaa ctcaacagca
    55141 aatacttcac gccgcgcaga ggaatatcca gccagcgggg aaggacacat tgccgaccaa
    55201 acagtttgtt accgagatcg ccgattaatt cagaaagcgt accaaccggg cataaccatg
    55261 agcaaaaggc ctttttgagt aatagactga tgacgataaa agcgaccaat aacagcatcg
    55321 cggcggcgtg gacggacggt aactgacctg ttacaaggct atatttcaga ttcatcagcc
    55381 cggcaatcgg tagccagcct tcgatacctc ccggtctggc gacaaatgtc gtgctacttg
    55441 ccgtttcgta atagcgcacc caataccaga acgtgatggc aatataaata ttcattgcca
    55501 acagtaataa ttgcgtcgct ttacgccagg tcgtggcatt acgccagtca ttccacggta
    55561 atttgccgcc cgtcgtgcct ggccgccgct gccagcgggt tcttttattc tctgccatga
    55621 ttttgccagt ccgttaagtt gtataccaaa tgccactatt ctagttgttc ttaactggct
    55681 gatattgatt caaatcgcgt tcaggtcttt cttatgcaac catgcttcca gagcggcaac
    55741 actgcgtgta atttcttcgt gtggaagggg ggcagataat ggctgctgct ccagttgtgc
    55801 gcatagctgg ctggcgatac gcattcccag actcgagcaa ctgcttttta gctgatgcgc
    55861 ggcacgcttt attttctcgc tatcctgact ggcgcgggca atgtcgattt catcgagaag
    55921 cggcagggca tgttgtgtaa ataataccag ccattcgtgg atcttctccg tccccattaa
    55981 ctgagcatct tcattgagtt gcgatacatc cagcgattga tcgttattga cttgccgttg
    56041 gagatagtgc gccagtaact gaccgagcac ttcacgcggc accggtttag ggataatccc
    56101 gcggaataat gaactggtac gctggcgcag cgtttcgtca atgacatggg cgctaaagcc
    56161 aatcaaaacc agcgacggat attgctgtgc cagttgtcgg gcaagcgtaa tgccgtcgac
    56221 atccggcaga tcaaaatcca ccagtgcggc agcaaacggt tcgctatttt gcagtgtctc
    56281 taaagcctgc gcggcattgc caacagcaac aacctgcgca ccactggtgt tcagcatctc
    56341 aacggtaatt cgctgggtta gcgggttatc ttcaattaac agcaaacgta aaccgtcaag
    56401 acgcaccgcc tgattgactg tttttggcac gggtgccgtg gcaacacgta acggcaagcg
    56461 taaacaaaaa cagcttccaa cctccggcgt gctggtggcg ctcagttcgc cgcccatcgc
    56521 ctgggccaga cggctactga tagtcagccc cagcccggtg ccgccgcgtt tgccgcttac
    56581 ctgcacaaat ggctggaaga tttctgccag tttcgcggga tcaataccgc agccgctgtc
    56641 ttccacttcg accagccatt gctcgccatc agtgcgacta cgcaggataa tgtacccttc
    56701 gtcagtaaaa cgcagggcgt tgctcaacag gttggttata acctgacgaa tacgtcgtgg
    56761 atcgcccatt aacgcgcacg gcatatcatc ggcaattgcc gttgccaggc gaatcgggcg
    56821 acctttcacc cgtccgctca ttaattgcag ggtactttcc agcagcgggc gcggttcaaa
    56881 gggctcatcg ctgaccgaaa cattcttgcc acctgcttcg atagcggaat aatcgagaat
    56941 atcgttgagg atggtcagca acgattcacc agagtcagta attgcccgca aatcatcacg
    57001 ctgggcgtta agtgcggggt tatctgccag cagttgagca gtgccgagaa taccgtacag
    57061 cggtgtgcgg atctcatggc tcatcgccgc cagaaacgcc gattttgcct ggctggcttt
    57121 ttctgcttcc gcccgtgcct gtcggtgttc tatcaccagt tcctgcaatt cagctgtacg
    57181 cgctttgacc tgcgccgcca gctgttcgcg gtggcgattc agtgcatgaa cattgctgcg
    57241 aaacgcatcc atcagccgcc cgatggtatc cagctcccgt acgccagcgg tttccgggaa
    57301 aggggagtca atatcaccgt ccagcagccg ttgcagcgcc tgcgtttgtt cggcaagtgg
    57361 acgcgtgact gagcgataaa ccacgcgcca aaggatcaga atcagtgcgc aaagtgaaac
    57421 catccccagc aataacaggc tgtattgccc gcgtgcactg gctttttcca gatgcgccag
    57481 tccgtgctga ttacgcagct caatggtgtc gaccagctga ctgacttcgc tactaaactg
    57541 cgcgaactgg gcgatgttat tttgtgcgag agtttgtagg tgattgctga tttcactgtc
    57601 ctgctgatac agcgccagca aatcgctata ttggctaacg gtagttaacg ttgttgcgac
    57661 ctgcgcacga acacccggat cttcaatgcg tatttgccga cgttgcagaa ttttcaccgc
    57721 attattgagc tgcttttcca gcgttggtgc atttttctgg atctgctcca gccccagatt
    57781 catcaccatt tgctgcaccc gcagagcgct aaggcgcagt tcattcatct ggttaacata
    57841 ctcaagatcg atatcaatca gccgatcgag tgcactttca gcagcctgac gctgatcttg
    57901 ttcgatcaaa tcgtaaatcc cggcctgggt cgctccagcg gaagttgtcg cattattcgc
    57961 ctgaccttgc gccaggcgtg cgatctcatc ggcggcagcg actatctgct gactgagttg
    58021 ccgttgttgc tggcgtagtt gcagacgccg ccccaccagt tccccttgct gacgtaatga
    58081 acgggagatc tcctgctcct gttgttcaat agcggtggta tcaaaacctt gttcccgtaa
    58141 cgcttgcagc aacgcattaa tcttcaggct ttgtgcggtg agcattcgcc cctgcgcctg
    58201 ccacatcttt tcgttatcgg cactggtcag gttctgcgcg gcgaaaagtt cccaggcgct
    58261 ggcttcgctc aactggcgcg ccatattcat ggtaggaatc aatgcctgag tgttgtcttt
    58321 ttccacctgg ctgataaagc gcaggttgta ccatcccacc agggtactgg tcagggttaa
    58381 cagcgccatc agggcaaagc ccatccagag tcttcgggtc agggttaaat tcacggtcgg
    58441 tgcacttaag gtgaaaaagg ttgagtcgca aagcggaatg catctagcat aaagccttat
    58501 tattgatgag gctatcatgc gcgtactgct atttttactt ctttcccttt tcatgttgcc
    58561 ggcattttcg gctgataacc tgttgcgctg gcatgatgcg cagcatttca cggtgcaagc
    58621 ctctatgccg cttaaagcca aacgcgcatg gaaactgtgc gcgctttatc ccagcctgaa
    58681 agattcatac tggttatcgt tgaactatgg tatgcaggag gcagctcgcc gctatggtgt
    58741 ggatttaaaa gtgctggagg caggcggcta cagccagttt gctacccagc aagcacaaat
    58801 cgaccagtgt aaacagtggg gcgcagaggc cattttactc ggtagtagca cgacctcatt
    58861 tcccgatctg caaaagcagg tagcaagtct gccggtgatc gaactggtaa atgctattga
    58921 tgctccccag gtgaaaagcc gcgttggtgt accctggttt cagatgggct atcaacctgg
    58981 gcgatatttg gtgcaatggg cgcacggtaa accactgaac gtgctgttga tgcccggacc
    59041 cgataatgcc gggggcagta aagagatggt cgagggtttt cgcgcagcca ttgccggaag
    59101 cccggtgcgt attgttgata ttgcgcttgg tgataacgat attgaaatcc agcgtaacct
    59161 gttgcaggag atgctggaac gccacccaga aatcgacgtc gttgccggaa cggccattgc
    59221 ggcagaggcg gcaatgggtg aagggcgtaa cctgaaaacg ccgcttaccg tggtgtcgtt
    59281 ttatctttca catcaggtgt atcgcgggct gaagcgggga agagtgatta tggctgccag
    59341 cgatcaaatg gtctggcagg gggaactggc agttgagcag gccatcaggc aattacaggg
    59401 gcaatcggtg tctgataatg tcagcccacc gattttagtt ctgacgccga aaaatgccga
    59461 ccgagaacat atccgccgct cgctgtcacc agggggattt cgtccggtct atttttatca
    59521 gcacacatca gcggctaaga aataaccttc accatgttgc gtcaccagta aatccgcgct
    59581 gagtttatga cgtaaacgac gaattaacac atcgacggtg cgcaggtcag ggttttccac
    59641 ccgacgcgca gaaagcatac gtagcagacg ttcacggctg agaatttcgc ccggattcgt
    59701 cacaaatgcc accaacattt catactctgc gcgggtcagt ttaatcggct cgccatcccg
    59761 ctccagcgta tggcgcgaca cattcaggca ataaccggca aagcgatagc agttgtcctg
    59821 agtgtgcggt tgagcttgtc gcgcgaggtc gattcgccag agcagatttt tcacccgtac
    59881 taccagttcg cgcagttcca gcggtttggt gacgtaatcg tctgcgccca tttccagccc
    59941 aacaatacgg tcaatccgat cgctgcgtcc ggtaaccaga ataatcccca ccgttgagcg
    60001 ttctcgcagg gcgcgggtta acatcaggcc attttcatcg ggtaagttga tatccagcag
    60061 aattaaatct accggctgat tctgcataat ttcccgcagc ccggcaccgc tcgctgtaac
    60121 ggaaacggta tacccctcct gagtgaagta agattgtaat cgcgcctggg taaccggctc
    60181 atcttcaaca ataacaatgt gatgtggcat cagagggttt tactcattct gttcatatct
    60241 gttcatattc tgccgtaagc cgttcatcct gaccagtgcc gctgttcata tttgctcatt
    60301 aagatcgctt cactaaacca taattttaca ggggctatta tgcggaaact ctggaacgcg
    60361 ctacgccgac ccagtgctcg ttggtcggta ctggcgctgg tcgcaattgg gattgtgatt
    60421 ggcattgcgc tgattgtatt gccacacgtt gggatcaaag tcaccagcac aaccgaattt
    60481 tgtgtcagtt gccacagtat gcaaccggtg tatgaagaat ataaacagtc ggtgcatttc
    60541 cagaacgcct ccggcgtgcg agctgaatgc catgactgtc atatcccgcc ggatattcca
    60601 ggcatggtga agcgcaaact ggaagcgagc aatgatatct accagacctt tattgctcac
    60661 tccattgata cacctgaaaa attcgaagcc aaacgcgcgg aacttgccga gcgtgaatgg
    60721 gcgcgaatga aagaaaacaa ctcggcaacc tgccgctcct gccataacta cgatgcgatg
    60781 gatcatgcga agcagcatcc tgaagcggca cgtcagatga aggtggcagc gaaagataat
    60841 caatcctgca tcgactgtca taaaggtatt gcccaccagt taccggatat gagtagcggc
    60901 ttccgtaagc agttcgatga gctgcgcgcc agtgctaatg acagtggtga cacgctgtac
    60961 tctattgata ttaagccaat ttatgcggcg aaaggcgata aagaagcctc tggttctctg
    61021 ctgcctgctt cggaagtgaa agtccttaaa cgtgacggcg actggctgca aattgaaatt
    61081 accggctgga cggaaagcgc cggacgtcag cgtgtactca cccaattccc aggtaaacgc
    61141 atctttgttg cctcgattcg tggtgatgtg cagcagcagg taaaaacgct ggagaaaacc
    61201 accgttgccg acaccaatac cgagtggagc aagttgcagg ccactgcgtg gatgaagaaa
    61261 ggcgacatgg tgaacgatat caaaccgatc tgggcttatg cggattcgtt gtacaacggc
    61321 acctgtaacc agtgccacgg cgcaccggaa atcgcccact ttgacgctaa cggttggatc
    61381 ggcacgctca acggcatgat tggctttacc agtctcgata aacgtgaaga acgcaccttg
    61441 ttgaaatatc tgcaaatgaa tgcgtctgac accgcaggta aggctcacgg cgataagaag
    61501 gaagaaaaat aatgaacaat aacgatctct ttcaggcatc acgtcggcgt tttctggcac
    61561 aactcggcgg cttaaccgtc gccgggatgc tggggccgtc attgttaacg ccgcgccgtg
    61621 cgactgcggc gcaagcggcg actgacgctg tcatctcgaa agagggcatt cttaccgggt
    61681 cgcactgggg ggctatccgc gcgacggtga aggatggtcg ctttgtggcg gcaaaaccgt
    61741 tcgaactgga taaatatccg tcgaaaatga ttgccggatt gccggatcac gtacacaacg
    61801 cggcgcgtat tcgttatccg atggtacgcg tggactggct gcgtaagcgc catctcagcg
    61861 atacctccca gcgcggtgat aaccgttttg tgcgcgtgag ctgggatgaa gccctcgaca
    61921 tgttctatga agaactggaa cgcgtgcaga aaactcacgg gccgagtgcc ttgctgaccg
    61981 ccagtggttg gcaatcgacg gggatgttcc ataacgcttc ggggatgctg gcgaaagcta
    62041 ttgccttgca tggtaatagc gttggtacgg gcggagatta ctctaccggt gctgcgcagg
    62101 tgatcctgcc gcgcgtggtt ggctcaatgg aagtgtatga acagcaaacc tcctggccgc
    62161 tggtattgca gaacagcaaa accattgtgc tgtggggctc tgatttgctg aaaaaccagc
    62221 aagcgaactg gtggtgcccg gatcacgatg tttatgaata ttacgcgcag ctaaaagcga
    62281 aagtcgccgc cggtgaaatt gaggtcatca gcatcgatcc ggttgtcaca tccacccatg
    62341 agtatctggg gcgcgagcat gtgaagcaca ttgcggttaa cccgcaaact gatgtgccgc
    62401 tgcaactggc gctggcgtat acgctgtaca gtgaaaacct gtacgacaaa aacttcctcg
    62461 ctaactactg tgtgggtttt gagcagttcc tgccgtacct gctgggtgag aaagacggtc
    62521 agccgaaaga tgccgcatgg gctgaaaaac tgaccggcat tgatgccgaa accattcgtg
    62581 ggctggcgcg gcagatggcg gcgaacagaa cgcaaattat tgctggctgg tgcgtgcagc
    62641 gtatgcagca cggtgaacag tgggcgtgga tgattgtggt tctggcggcg atgctggggc
    62701 aaattggcct gccaggtggt ggctttggtt ttggctggca ctataacggc gcaggcacgc
    62761 cggggcgtaa aggcgttatt ctgagtggtt tctccggctc tacgtcgatt ccgcctgttc
    62821 acgacaacag tgattacaaa ggctacagca gcaccattcc gattgcccgt tttatcgatg
    62881 cgatcctcga accggggaaa gtgatcaact ggaacggtaa atcggtaaaa ctgccgccgc
    62941 tgaaaatgtg tatttttgcc ggaactaacc cattccatcg ccatcagcag atcaaccgca
    63001 ttattgaagg ctggcgcaag ctggaaacgg ttatcgccat agataaccag tggacctcaa
    63061 cctgccgctt tgccgatatc gtgctgcctg cgaccacgca gtttgagcgt aacgatctcg
    63121 accagtacgg caaccactcc aaccgtggca ttatcgccat gaaacaggtg gtgccgccgc
    63181 agttcgaggc gcgcaacgac tttgatattt tccgcgagct gtgccgtcgc tttaatcgcg
    63241 aagaagcctt taccgaaggg ctggacgaaa tgggctggct gaaacgcatc tggcaggaag
    63301 gtgtacaaca aggcaaagga cgcggcgttc atctgccagc gtttgatgac ttctggaata
    63361 acaaagagta cgtcgagttt gaccatccgc agatgtttgt tcgccaccag gcattccgcg
    63421 aagatccgga tctcgaaccg ctgggcacgc cgagtggcct gattgagatc tactcgaaaa
    63481 ccatcgccga tatgaactac gacgattgtc aggggcatcc gatgtggttt gagaaaatcg
    63541 aacgctccca cggtgggccc ggctcgcaga agtatccgtt gcatctgcaa tctgtgcatc
    63601 cggatttccg acttcactcg cagttatgtg agtcggaaac tctgcgtcag caatatacgg
    63661 tagcgggtaa agagccagtg ttcattaacc cgcaggatgc cagcgcgcgc ggtattcgta
    63721 acggtgatgt ggtacgcgtc tttaacgctc gcggtcaggt gttggcaggg gcagtagttt
    63781 ctgaccgcta tgcacccggc gtggcacgaa ttcacgaagg ggcatggtac gatccagata
    63841 aaggcggcga gcctggtgcg ctgtgcaaat acggtaaccc caacgtgctg accatcgaca
    63901 tcggtacttc gcagctggcg caggcgacca gtgcgcacac tacgctggtg gaaattgaga
    63961 agtacaatgg agcagtggag caggtgacgg cgtttaacgg ccctgtggag atggtggcgc
    64021 agtgcgaata tgttcccgcg tcgcaggtga aatcatgacc acgctgacag cacaacagat
    64081 tgcctgtgtt tacgcctggc tggcgcagtt gttctcccgt gaactggacg atgaacaact
    64141 gacgcaaatc gccagtgcgc agatggctga atggttttcg ttgctgaaaa gcgaaccgcc
    64201 gctggctgcg gcggtgaatg agctggaaaa ctgtattgcc acgctgacag tacgtgacga
    64261 tgcccgtctg gaactggccg ctgacttttg cggcctgttt ctgatgaccg acaaacaagc
    64321 ggcgctgccg tatgcatcgg cctacaaaca ggacgagcaa gagattaaac gcttgttagt
    64381 tgaggcaggg atggaaacca gcggcaattt caacgaaccg gcagatcatc tggcgatcta
    64441 tctcgaattg ctcagccatc tgcatttttc gctgggagag gggaccgttc ctgcgcgaag
    64501 aatcgacagt ttgcggcaaa aaacactgac ggcgctgtgg caatggttac cagagtttgt
    64561 tgtgcgttgt cgtcagtatg acagctttgg tttttacgcg gcactaagcc agttattgct
    64621 ggtgttagtg gagagcgacc accaaaacag ataacgtcgt ttgtgcgcct gaaaagacgc
    64681 gtttagcgtc gcatcaggca ttatggggca gttgccggat gcgtcatcac atccggcaaa
    64741 cacacatcac ggatgagcta caaaccggga aagtcgctgg cgcagcaggc ggttttcctg
    64801 cttcaggtgc gcaatatcat ccattaacgt cagcgccacc gcgatcccag gccagtccag
    64861 agccagttca tgacgcaggc gtactgcgcg ttgcaccacg atggcggcat ggtcgtcaaa
    64921 tacccaggtt gtttcctgga tctcacgcgg ttcaaccacc cccaaaccga caatttcatt
    64981 caactcctct tcagagatgc cggtatgcag gcaaaattcg gtaatagtaa aagtcaccgt
    65041 aacattagcc attatgcttt cccccaatct ttacgtggat caaaagacga ctgggcgtct
    65101 gccagttgct gccacagcgc ggcagtgttt tcatccggtt tcggcggcat cacgattttc
    65161 agtaccgcat acagatcgcc ggtctgtttt ttgctcacca gacctttgcc tttaacgcgc
    65221 aatcgttgcc cggcctggct gcctggcggg atagtcagca aaatgctttc tttcagtgtt
    65281 ggaacggtga ctttagcacc cagcgccgct tcccacgggc taaccggcac cacaatttcc
    65341 agatcctggc cgacaatatc gaacagcgga tgtggcgcaa tatgaatcac cagccacaaa
    65401 tcgccatttg gaccaccgtt ttcgcccggc gtcccctggc ctttcagacg gatgcgttga
    65461 ccattgccga cgcccgccgg gatcttcaca ttcagcgttt tcgggatttc ctgttcgatc
    65521 atgccaaagg cgttataaac cggcaggtta tagctgatgg tacgcttatg ctcagtcagc
    65581 gtttcttcga ggaataccgc cacttcgatt tcaatatcgt ggccgcgtgc ggcggggcgt
    65641 tgacggctct ggcgggcatg ctgaccgaaa attgacgaga agatatcgtc aaaatcttcg
    65701 gcattaaaac tctgaccgtc gccatgatgg aactgacggt taaattgcgg atcgttgcga
    65761 tgttgccaca tctgatcata ctcagcgcga cgttgttcat cacttaacac ttcccaggct
    65821 tcagcgacct ctttgaagcg ggcttcggca tccggttctt tgctgacatc aggatggtat
    65881 ttgcgggcca gtcgacgata ggcggtcttg attgtcttga gatcgtccgt cggtttcacg
    65941 cccatgatgg cgtaataatc ctttaattcc atagcgttat ctcgcgtaaa tcaacacaaa
    66001 ttgaaggaac ccctgtaagg taactcctat aagtgtaggg taatcctcaa aatttcatat
    66061 gccaacacag aatatgttat tgaaatcatc gcggagagga ggtcgcctca aggtggggtg
    66121 ctgaacatat tttaaacagg tgaaaaaggg tgagcgattt ttgatagtgg aaccaggcac
    66181 tttaagttta actggggcgt cattatttat taaattttat aggcgctata tatgagtagt
    66241 aatatacatg gaattagttg cactgcaaat aattatttga aacaagcctg gaacaatata
    66301 aaaaatgagc acgaaaaaaa tcaaaaatat tcaatcacgc tttttgaaaa cacactggtg
    66361 tgttttatgc ggttgtataa agaaatcaga cgtcaaaaag ctgaagacta cattccatgc
    66421 ctggaatgcg attcactaga aaaagaattt gaggaaatgc agaatgataa tgatctatca
    66481 ttatttatga gaacattgcg tactaatgat acacaaattt attcaggggt ttcaggaggt
    66541 attacatata ctatacaata tgttcaaaat gttgatattg ttagagtgtc cttgccgggc
    66601 agaggttcag agtctatcac agattttaaa ggttattatt ggtatggctt tatggaatat
    66661 attgaaaaca ttactgcgtg tgatgatgtt ttttctgagt attgtttaga tgatgaaaat
    66721 atgagtatcc agccagaaca gataaatacg ccgggaatat ctgatttaga ttctggtatt
    66781 gatttgtctg gtatatcttt tattcagagt gaaattaata aaacttatgg attaaaatac
    66841 gctccagtag atggcgatgg ctattgtctg ttaagggcca tactggtttt aaaagaacat
    66901 gagtattcat gggcgctggg cagccataag acgcaaaagc aagtatacga agagtttatt
    66961 aaaattgttg ataaacaaac gatcgaggct cttgttgata cggcattcta taatctcagg
    67021 gaagatgtaa agacgttatt tggcgttgat ctacaatctg acaacaaaat tcagggggag
    67081 ggtagtctta tgtcatggaa ctttctgttt tttaaaaaat aatttattga tagttgcttg
    67141 aatgacaaaa aatgcatact gcatttaccc gagtttatat ttaatgataa caaggcacgc
    67201 cttgttttag acaccgaccc agagaagagg attaatgagg tgaaaaattt cctcactgct
    67261 ctttcagata gcatttgctc attatttatt gttaatagta atgtggcatc aatctcctta
    67321 gggaatgaat ccttttcaac agatgaagat cttgagtatg gttatttaat aaacactggc
    67381 aatcattatg atgtttacct ccctcctgaa ctttttgctc aggcttacaa gttaaaaaat
    67441 aaggaaatga atgcgcaact cgactattta aatcgttatg caatttaatg gcaaaggcat
    67501 atgctaaaaa ccattgttat tagtctcaca cttttttatt ggtaaatatt gtctctgtat
    67561 tggtaacgcc gcagatattc tgtttagcca caggtgcaat tatcagcggc gtacgcgagg
    67621 caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttaaaga tgtatataga
    67681 aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt catatttctg
    67741 tcacactctt tagtgattga taacaaaaga ggtgccagga atgaacaaaa cgctaatcgc
    67801 cgcagctgtg gcagggatag ttttactcgc ttcaaacgct caggcacaaa ccgtaccgga
    67861 aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg cgccgctggc
    67921 gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat gggacgtccc
    67981 cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc attacatgcg
    68041 tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc cgtacaccgt
    68101 ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct ttattaccgg
    68161 cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca ctatggaccc
    68221 aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg cggtggcggc
    68281 aatggagaaa gagctcagca aactccagct taccgacagc taccagctac tggaaaaaat
    68341 cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc tggtggatgg
    68401 caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc cgctgaaagt
    68461 cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc cgatggatca
    68521 ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga agcttaaaaa
    68581 cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg cgaaaccgct
    68641 ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga aaattacagt
    68701 gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt tcaaaccgta
    68761 tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt tccagcgttg
    68821 gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc agagtgcgga
    68881 acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg tgacgctgga
    68941 attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat ttgatagcgt
    69001 gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg agtcgctggt
    69061 taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt atcggtctta
    69121 gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc cgccattttt
    69181 tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt attgcgcaga
    69241 gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg tatcctcctg
    69301 ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct tgataacgag
    69361 caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg atggtggttg
    69421 cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc gctgcgtagc
    69481 caatggggac aattaccatg ccgtgatgcg caagggtcgt ccaggttgaa gtaatagttt
    69541 gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt ccgtatagtg
    69601 cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga ccggacatgt
    69661 tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt tcttgcgggg
    69721 ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc ggcggcatgg
    69781 tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca ccctcagcga
    69841 ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcacc agaactttag
    69901 ccatttctaa ccactcctcg tgttatctct attccgtagc gattcgctac cacttattta
    69961 aagataagac gtccttttca gagtgcaaat ttcacaacca cttatttgat ttataacaac
    70021 tttcacaagc acgtaatttt gtcgcaaaat gacacatttt tatctcatcg cgttttttta
    70081 atcataagag cggcttatgg ataattattt gagatgatat ctattctcgc taagaagctg
    70141 ttgcaggata ttaccaaacg cgggtctgcc cgcgtcagtt cactaagctt agtcccacgt
    70201 agcgaaaata tggcagccgc catacgccgc gttaattcta tgcaatatga tgtctatacc
    70261 cagacggagg tcagtaatgg caaaccatcg aggcggttcc ggcaattttg cagaagaccg
    70321 cgaaagagca tcagaagcag gtaaaaaagg tggacagcac agcgggggta atttcaagaa
    70381 tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc acggcaaaag
    70441 cgacaactag ccgggctaat caatgacgaa tgcatttttt tcttaggctc gtcaaaaagt
    70501 catcaccgcc ggtaatcccg gcggttttct gtttatggct cctgatgaac aacttctggc
    70561 ggtggaacgt caaccaactt tctgcttaac aacgcattga gtaaaatcgc gccaaaggtt
    70621 gctgtaccaa tccctcccaa cgtaaaaccg cccagcgtga gagcaaaatc acccgcgccc
    70681 agcactaagg ttactgcgac cataatcaaa ttaccgttct ggcttaaatc gacacggttt
    70741 tgtacccata tccttgcgcc tgcgacggca atcagcccga acacaacaat tgatgcacca
    70801 ccaatcaccg cggccggaat ggtatgaatc agcgcgccaa atttcggtga aaagcccaac
    70861 agcatggcga tgacggcagc agcgacaaac accagcgtcg agtagacttt tgtcacggcc
    70921 attacaccga tattctcggc gtaggtggtt acgccgctgc caccgacaga accagaaagc
    70981 atcgtcgcca gcccatcacc gacgaatgcc cgccccatat acgggtccat attgcgtccg
    71041 gtcatcccgg cgaccgcctt gagatgacct aagttttccg ccaccagaat caccgccacg
    71101 ggcgcaatca gcatcattgc ctgactatta aaagcaggag tggaaaaatg tggcaaaccg
    71161 aaccaggcag catggctgac gagagtaaaa tcgacggctt ttcccagccc taaaacgttg
    71221 gtcatcacgc catacagcag acaggcgaca attaaaccta cgagaatcaa taaccgctgg
    71281 atcatgccac gggtaaacac cgccaccagc ccaatacaca gcaccgtcat taccgccatc
    71341 cagctatcaa aggccgaagc cgatacactt ttcactgcga taggcgctaa gttcaggcca
    71401 atcgccatca ccaccgcacc cgtcaccacc ggcggcatta gtcgttcaat ccagcgtgta
    71461 ccaattttca tcaccaccag gccaatgacg gtataaacca gcccacaggc gataatcccg
    71521 cccagcgcaa tgctgatatt cgggttaatg ccctgaccgt taaagcccgt cgcggcaatc
    71581 accacgccga caaaagccgc gctggagccg agataactgg ggacacgccc gccggtaata
    71641 aagaaaaaca gtaacgtgcc gatccccgac attaaaatgg aaagattggg atccagcccc
    71701 atcagaatcg gcattaacac cgtcgcgcca aacatcgcca ccgcgtgttg aacgcccatt
    71761 attgccgtct gagcaaacgg caatcgttca tccggcgcga ccacgccgct ctctgtagag
    71821 gtcgatttta actgccagtg aggaaaaccg aacattgcca tcagctgtct ccttaaggag
    71881 gttaacaagc agggcgcatc agcgcgtgat aactgcgatc gaaccacacc agcccgtagg
    71941 gtgtggcgtg acgatgaata gcttcgatgg cgcaaaacaa aatgtcgtgg gtgccgacgc
    72001 tcaccacctg gctgatacgg cagtcaaacg aaaccagtgc ctcttccagt tgcgggcatc
    72061 cggtcacacc tgtctgccag cgggcggcgg caaagcggtg ttccatgggc gttttgccgc
    72121 caaaaaggtt ggaaagcggc tcctgcccgg cgctaagtgt atttacacac agcgttcgat
    72181 tttcattgaa taccggccag acggacgccc cacgattcag gcacaccagt aatgtgggcg
    72241 gcgtatcggt cacgctgcag acggcgctgg cggtgaaccc ggcgcgcccg gctggaccgt
    72301 ccgtggtgat gatattgacc gccgcgccca tgcaggacat cgcatcgcga aaagtttgtt
    72361 gatcgacaat gttcatagtt tgctccttac aacagcccgc aggcttcttc aaaggacaga
    72421 cgtggcaggc gcgcaaaaag tttgcttcta tcgccatagc cgatattaat cagcagattg
    72481 ctcttcagcg tgctgcccgc aaaaaaggcg tcgtcaacgt gttgacggtc aaaacccgac
    72541 atcgggccgg tatccagtcc cagcgcacgg caggcgacga tcagataggc cgcctgcatg
    72601 gaactgttgc gaaacgctgt ttcttccgca agttgtgggc tggaggtaaa ccaactgcgg
    72661 gcatcgccgt gaggaaacag ttgtggtaac cgttcataaa attcactgtc ccaggcaacg
    72721 atagcggtga cgggcgcggt cagggttttt tgtagattgc cgctggaaag cgccgggcgc
    72781 agacgttctt ttccttctgc agtgcggata aacacgatcc gtgccggaga acagttagct
    72841 gatgtcggcc cccatttcat cagggcataa agctcccgta acgtctcatc gctgacgggt
    72901 gtctcccgcc agccgttgtg agtgcgggca tcggtgaaca gggtgctaag cgcacctggg
    72961 ctaacggctt cgttcatagc aattccttac agggcggctt cacggtgatg taacaggctg
    73021 gcaagcccgt tgagtaacag agcattaaac gtttcgggat cggtcacgtt gcaggcgtgt
    73081 ccgccatagc gcatcaccat tttctggcta tcgggcaggg cggcatgaag ttcactggaa
    73141 catgcggatg gcaccagcag atcatcactg gcgcagatga tttgcaccgg gcagcggatg
    73201 cgatccgcat ggtgactaaa gtcagcgcgt ttgagggcgt taagtcgacg cagtaaatta
    73261 tttttgccct gaaaatgcgc cagtgccagc gcgtcttctg cctccaggcg aggtgcgcgg
    73321 gccgccatcc agtcggcggg atagaggaac aacggctgcg cttccaccca tgcctgtgcg
    73381 ccgccgctat acagtaatcg ttcgcgaacc tgaaaacagc ggcgcgtatg ggcgtttatt
    73441 cgtagccagc cgttaacgct gaccagcata gttaccgacg cgggataatc cagcgccagc
    73501 tgcatcccca ccagcgcacc gagcgcatgg ccgaccaccg cgtaacgctc aatccctgcg
    73561 gctaccagcg cctgatgcag ttccgctgcc atctgggtga tactgtaatc ttctgccagc
    73621 gtgtcgggat tattgccggt gccgcgctgg tcgtaacaga ctacctgata ctcctgctcc
    73681 agcaccgcca gttgcggtaa ccagtaactg ccgctaccgc caagacccga aatcaacacc
    73741 actacgggtg catcagcata agggggaggt gagagtgata gtttcatcgc ggcctcactt
    73801 ggcgatatgc gcaattgtgg cgatttccac cagcgcgtca ggttttacca gtccgcactg
    73861 aatgcagaat cgcgccggtt tatcacccgg aaaaaactcg gcgtagattt cgttaatcgc
    73921 ggcgtaattt ttccagtcgg taataaagat gctgttgacg gtcacatccg ccatcgtgcc
    73981 acccgccgtc tcgatcacct tgcggatagt ttccagaacg tggcgggttt gcgcctttgg
    74041 gtcatcggca aacagcacgt tattatgttg atcaaaagcc agcgtaccgg agacatacac
    74101 cacgccatca gccagcgtgc cgggaacgaa gggggccagc ggtgcgctgc tgccagcggg
    74161 aataattacg gattttggca tcgttaaact ccttaagcga tacgagcaaa ggacgtggga
    74221 gaaagcgcgt cgcagaacgt ttcgacgtcg ctgacccagc caaaaaaggt ttcgatattg
    74281 aacaacgcgg ctttctgtac aaattccggc cccgcctggt gtgttgcgtc ttccagcacc
    74341 acgccgaaat actccagaaa aaagccatcg cgtagcgtcg attcgacgca gacgttggta
    74401 gcgataccgg tgaaaaccag atggcgtatt ccacggctgc gcaaaatgct gtccagcggc
    74461 gtattgaaga accgctgtag cgcggcttcg gcagcacaat atcgccaggc tgcggcacca
    74521 gttcatccac cagttgataa tcccaggagc ctttcgccag caatttcccc tgcagctgcg
    74581 gctgcttacg catggttttc agggcgttcg atttatgaaa attcggtgag ccgggtccgc
    74641 ctgcttcgac atactgttca tcccagccat tttgaaacca gatgatcagc atccctgccg
    74701 ctcgcgctgc ggtcacggcg gtttgaatgt tggcaatgac cgggcgagtg gttgagacat
    74761 caaacccggc gagatctaag tagcctcctg gcgtggcata agcgttttgc atatccacca
    74821 cgatcagcgc actttgctgc ggatcgaagg taatggcttc cggtcgagcg gttaaggtcg
    74881 tcatcatgcc acctcctgag tcagcgcagg gagatgggcg cggcactgca tcagtggttg
    74941 aatgcgctcg ccgaaggttt cgattcccga cagaaaatca tcgaaggtca acagcacgcc
    75001 ttcggcacca ggcacgcttg cgacttcatc taacatgcgc gcgacactgg cgtaagaacc
    75061 gactaacgtc cccatattga tgtttaccgc cgaagtggga tcggccatct gacggacgtt
    75121 ggtgtcagta ccggagcggg tatctttctg actttgttcg gttagccagc ttaacgcctc
    75181 ttcatccgcg cccgctttgt agtgttccca tttggcgcga gcggcatcgt cggtttcatc
    75241 ggcaatcacc ataaacaata cataagagcc aacgtcgcgc ccggtttgct ctgcggcctg
    75301 tttcatccgc gcagcggtcg gggcgaaagc cgtgggtgta tttacgcctt tgccgaaaca
    75361 gaagttgaaa tcggcatact gggcggagaa cgccatgcca gcgtcgcttt gcccggcgca
    75421 gatcactttc atgggtacac tcggttgcgg actgacgcga caatcattca tggtgaaaaa
    75481 atcgccttta aaatcgcttt tccccgagcc ccacaggtcg cgcagcacct gaacatattc
    75541 ggtgagatag tcgtaacgac gggagaaata gtcatcgcca ggccagatac ccatctgctc
    75601 atactcgggc ttttgccagc cagtcacgag gttgacgcca aaacgcccgc cagagatgga
    75661 gtcaatggtt gcggccatac gggcgacgat tgccggaggt aacgttaagg tggcagctgt
    75721 cgcgtaaatc tgaatgcgtg aggtgacggc tgccagcccc gccatcaagg tgaacgactc
    75781 aaggttgtga tcccagaact cagttttgcc gccaaagcca cgcagtttga tcatcgacag
    75841 ggcgaaatcg aaatggtagt gctccgcttt ttgcacaatg gctttattca gttcaaaggt
    75901 cggcatgtac tgcggcgcgt gggtcgaaat gagccagccg ttgttgccaa taggtacgaa
    75961 tacgccaatt ttcatcatca acctctcttc gtctcgtaaa gtgaaagtca gacggggcac
    76021 tgcgtcctgc atatcctttt cagccgcgta atggcttgtt tgcaaagtgg atgccagttt
    76081 ttaaaaagtg aatgttatta atgtgttaat gctaagttat ctaaaatatc tggtaaaaag
    76141 tggactaaac ggtcaaaaca gttgcacata aaacatgcat ccgtgcgcga tgagagtgca
    76201 gaaggtcgag gccggacggg ggttttgcta tcctgttgcc aatctacaag aggggagagc
    76261 gcatgacgca aggcgcagtc aaaacaacgg gtaaacgttc gcgtacagta agcgcgaaga
    76321 aaaaagcgat tcttagcgca gcgctggaca ctttttcaca attcggtttt cacggcacaa
    76381 ggctggagca gatcgcagag ttggcgggtg tttcaaaaac caatctgctg tattactttc
    76441 cgtcaaaaga ggcgctgtat attgccgtgc tgcggcagat tctcgatatc tggctggcac
    76501 cgttaaaagc gtttcgtgaa gatttcgccc cgctggcggc gatcaaagag tacatccgtc
    76561 tgaagctgga agtctcacgc gattatccgc aggcttcgcg cctgttctgt atggagatgc
    76621 tggcaggcgc gccgttgtta atggatgaac tgacgggcga tttgaaggca ttaattgatg
    76681 agaaatcggc gctgattgcc ggttgggtca aaagcggcaa actcgcgccg attgatccgc
    76741 agcatttgat ttttatgatt tgggcttcca ctcaacatta cgccgatttc gcccctcagg
    76801 tggaggcggt gacaggcgcg acgttgcgcg atgaggtatt tttcaatcaa acggttgaaa
    76861 acgtgcagcg gattattatt gaggggattc gaccacgtta aagatgccgg aggaggttgt
    76921 aacatcctcc ggctacctgt ttaacctata gtcattaagc tggcgttacc gccagcggca
    76981 gcggtattca cactcagcga acgctcgata tacagccgtt ccagaaggat attgctttcg
    77041 ccacgggcaa aaccctgcac cgaaacaatt gcgccatccc gcgcggcaac ggcttcacac
    77101 aatgcgcgaa gctgatccga atcaccgtgg aagatcaccg catcaaacgg ttgagcggtt
    77161 atattttccg ctttcgccag ttgaatacgt tcgctgactg ccgatggcaa tgccttcact
    77221 agctgacgat gcagcgcgtc atccggccac agtacctggc tgcccaccgc cagcacggcg
    77281 gcgagctgag tcagcgcatc ctgctcatca tcggcaatac acaacacgcg ctcacgcggc
    77341 agcagcgtcc aggtgttgcg ttcacccgtc ggccccggca gcaatcgttg tgttcctgcc
    77401 tgcgccagct cgccatattg cgtacataac gcctgcaatt ctggacgatt tgctgcccat
    77461 tcccgcagtg catttagcgg ctgagtcaat gcggctttca actgtgcatc gaccggatac
    77521 tctgcatcct gacgcgcgag cgtcactgcc agcgcacttt ccgggcgatt cgccagcaga
    77581 cggtagagat agagcggacc gcctgctttc ggcccggtac cggacaaccc ttcgccgccg
    77641 aatggctgca cacccaccac tgcgcccacc atattacggt taacatacag gttaccaaca
    77701 tgggacgagc cagtgacctg ggcgatggtt tcatcaatac gcgtatggac accaagcgtc
    77761 agaccatagc cggaagcgtt aatctgctcg atcagctctg gtagctggtt acggttgtaa
    77821 cgcaccacat gcagcaccgg accaaagacc tctttttgca attcggcaaa gtcatccagt
    77881 tcgatcagcg tcggggcgac aaaggtgccg ctttgccatt cacgggcatc ttcgctgttt
    77941 tcccgcaccg cctggaacac cggacggcct ttgctacgca tggtctgaat atggcgctca
    78001 atattggctt tcgcttcgct atcaatcacc ggaccgatat cggtggtcag gcgacccgga
    78061 ttacccatcc ggcattcggc cattgcaccg cgcagcattt tcaacgtgtg gtcggcaatc
    78121 tcatcttgca ggcacagcac gcgcagtgcc gaacaacgct gacccgcact gtcgaacgcc
    78181 gaggccagca catccacgac gacttgttcg gtcagtgctg aagaatcgac aatcatcgcg
    78241 ttcatgccgc cggtttcagc gatgagcgga atagggcgac cctgagcgtc caggcggctg
    78301 gcgatattac gttgcagtaa cgtagcgact tcggttgaac cggtaaacat caccccgcgc
    78361 acgcgatcat cacccgtcag ttgcgcgccc acggtttccc cctgacctgg cagcaattgc
    78421 accacgccag ggggtacacc cgcttccagc aaaatggcga tcccttgcgc ggcaatcagc
    78481 ggcgtttgtt ctgccggttt tgccagcacg ctgttacctg ccgccagtgc ggcagcgatc
    78541 tgcccggtga aaatagccag cgggaagttc cacggactga tacacaccac aggccctaat
    78601 ggacggtggg tttcgttagc gaaatcatcc cgcacctgtc cggcgtagta gtggagaaaa
    78661 tcgaccgctt cgcgcacttc ggcaatggcg ttactgaagg tttttccggc ttcacgcacc
    78721 agaataccaa tcagttgctg catctggctt tccattagca cggcagcgcg atgcaaaatc
    78781 gccgcgcgtt cagccggagg cgtggcaaac cagattggcg cgttattaac cgcactttcc
    78841 agcgcctgtt ctacttcacg cggcgtggct tcacgcacaa agcccacaat atctttcggt
    78901 tccgcagggt taataacggg cgacatctca cctgccgcta ccggttgttc cagcattggc
    78961 aaggcctgcc atttttgcag tgcactattg agcagggcag aggagagcga ggccagacgg
    79021 tgttcgttag cgagatccag ccctgccgag ttgtcgcgcc cgtgaccgta aagatcgcgc
    79081 ggcaggggaa ttttcggatg cggtaatcca gtttgccctt cctgttgcgc cagtttttct
    79141 acagcagtga ccggatcggc gaccagttca tccagtggca aagaggtgtc ggcaatacgg
    79201 ttaacaaacg aggtgttagc accgttttcc agcaggcgac gcaccagata cgccagcagc
    79261 gtttcatgtg tgccaaccgg ggcataaata cgacacggtc ggttaagttt accgtcggca
    79321 actttcccgg tgacctgctc gtacagtggc tcgcccatac catgcaggca ctggaactcg
    79381 tactgacccg gatagtagtt ctgacctgcc agttgataaa tcgctgccag cgtatgggcg
    79441 ttgtgcgtcg cgaactgcgg gtagattaga ttcggcaccg ccagcagttt tttcgcacag
    79501 gcgagataag aaacgtcggt atacaccttg cgggtataaa ccggataacc ttcaaggccg
    79561 tccatctgcg cacgcttaat ttcactatcc cagtacgcgc ctttcaccag acgaatcatc
    79621 agacggcgac ggctacgggt ggcgagatca atcaggtaat caatcaccaa cgggcagcgt
    79681 ttttgataag cctgaataac aaaaccgatg ccgttccagc ctgccagttc cggctcgaaa
    79741 cagagttttt ccagcagatc gagggagatc tccagacgat cggcctcttc ggcatcgatg
    79801 ttaataccaa tatcatactg acgcgccagc agggtgagtg atttcagacg cgggtaaagt
    79861 tcttccatta cccggtcata ctgggcgcgg ctataacgcg gatgcagcgc cgacagtttg
    79921 attgaaatgc ccggcccttc atagatgcca cgaccgttag acgctttacc gatggcgtga
    79981 atcgcctgct gataggaaac catatacgcc tgtgcgtctg cggcagtcag tgcagcttcg
    80041 cccagcatat cgtaagagta acggaaacct ttctcttcca gcttgcgggc attggctaac
    80101 gcttccgcga tggtttcacc cgtgacgaac tgctcgccca tcaggcgcat cgccatatcc
    80161 acacctttgc ggatcagcgg ttcaccgctt ttaccgataa tgcggttcag cgagcgggag
    80221 aggatggctt cgttatgggt ggaaaccagt ttgccagtaa acagcagccc ccaggtggcg
    80281 gcattaacaa acagtgacgg gctacgacca atgtgtgact gccagttacc gttgctgatt
    80341 ttgtcgcgaa ttaacgcgtc gcgggtggct ttgtcgggaa tacgcaacaa cgcttctgcc
    80401 agacacatca gcgccacgcc ttcctgcgat gacagcgaaa actcctgcaa taacccctgg
    80461 accatacctg cgcgaccact ggcatttttt tgattacgca gtttatcggc cagttgatac
    80521 gccagtttgt gcgcctgtgc agcaactggc tgcggcaggc gggcttgttc cagcagcata
    80581 gaaaccgctt cggtttccgg gcgacgatag gccgcggtga tcgcggcgcg ggaaaccgac
    80641 tggggcaaaa tttgctcggc aaagtcgagg aatggctggt gtggttcctc tgccggagtc
    80701 ggtgcttcat cgctctcatt ggcagcgcca gaaagcagcg caggtagctc tggcagagta
    80761 tcgctgtttt ccagttgttc gagataagaa aaaatcgcct gcttaattaa ccagtgtggt
    80821 gtgcgatcga tacgtgtcgc ggcagactta atacgctcac gcgtcgcgtc gtccagctta
    80881 acccccatgg tggtggttcc catgccatta ctcctgttgt tctgaaaggt gcaacttaac
    80941 gttatcgtga aatatccatg atgttgcaac tttgtgcaac catgttaaat gtgacctgcg
    81001 tagcaagctt aaaaatgaat gaaatgttaa taaaagaaat cgatatgaca gggattaaaa
    81061 aaataaatca gactttttct ctgcggcagt taacattttt gaaaggtgca accgcaaaaa
    81121 atgtgagaga gtgcaacctg atgaaaaata gtgtcgctga gcactaaaat ttaatgtaaa
    81181 tggtgtgtta aatcgattgt gaataaccag cgcttccggc aggatacggt cgccctggta
    81241 aaacataaac tctgttaccc cgttccggtg gcagatataa cggcaagttt cgacattgcc
    81301 gataataatt ttttggagac tttagatggc tattagcaca ccgatgttgg tgacattttg
    81361 tgtctatatc tttggcatga tattgattgg gtttatcgcc tggcgatcaa cgaaaaactt
    81421 tgacgactat attctgggcg gtcgtagtct tgggccattc gtgacggcat tatcggcggg
    81481 tgcgtcggat atgagcggct ggctgttaat ggggttgccg ggcgcggttt ttctttccgg
    81541 gatttccgaa agctggatcg ccattggcct gacattaggc gcgtggatca actggaagct
    81601 ggtggccggg cggttgcgtg tgcataccga atacaacaat aacgccttaa ccctgccgga
    81661 ttatttcacc gggcgctttg aagataaaag ccgcattttg cgcattatct ccgcgctggt
    81721 tattttgctg ttcttcacca tttattgcgc ttcgggcatt gtggcaggcg cgcgtctgtt
    81781 tgaaagtacc tttggcatga gctacgaaac ggctctgtgg gcgggagctg cggcgacgat
    81841 cctttacacc tttattggcg gtttcctcgc ggtgagctgg actgatactg tacaggccag
    81901 cctgatgatt tttgccctga tcctgacacc ggttatcgtc attatcagtg tcggtggctt
    81961 tggtgactcg ctggaagtga tcaaacaaaa gagcatcgaa aacgtggata tgctcaaagg
    82021 gctgaacttt gttgccatta tctcattgat gggttggggg ctgggttact tcggtcagcc
    82081 tcatattctg gcgcgtttta tggcggcgga ttctcaccac agcattgtcc atgcgcgtcg
    82141 tatcagtatg acctggatga tcctctgcct ggcaggggcg gtggctgtcg gcttctttgg
    82201 gattgcttac tttaatgagc acccggcggt agcaggtgcg gtaaaccaga acgctgaacg
    82261 tgtgtttatc gaactggcgc aaattctgtt taacccgtgg attgccggga ttctgctgtc
    82321 ggcaattctg gcggcggtaa tgtcaacgtt aagctgccag ctgctggtgt gctccagtgc
    82381 gattaccgaa gatttataca aagcgtttct gcgtaaacat gccagccaga aagagctggt
    82441 gtgggtaggg cgtgtgatgg tgctggtggt ggcgctggtg gcgattgcgc tggcggcaaa
    82501 cccggaaaac cgcgtgctgg gcttagtgag ctacgcgtgg gcaggctttg gcgcggcgtt
    82561 tggtccggtg gtgctgttct cggtgatgtg gtcacgcatg acgcgcaacg gtgcgctggc
    82621 ggggatgatc atcggtgcgc tgacggttat cgtctggaag cagttcggct ggctgggact
    82681 ctacgaaatt attccgggct ttatcttcgg cagtattggg attgtagtgt ttagtttgct
    82741 gggtaaagcg ccgtcagcgg cgatgcaaaa acgctttgcc gaggccgatg cgcactatca
    82801 ttcggctccg ccgtcacggt tgcaggaaag ctaagggact tagcctgcgg cggttttgtt
    82861 tggcttcagc agcgggttgc gctcccttaa tgtgcctcgc catataaatt gaatggtgca
    82921 gggagcgcgc agggggcggc caatcgccgc cgccccctgc tgtcccggcc ttcggggaac
    82981 gcttcagcga ttttgacgcc accaacaccc gagctgttat tatgttccgg gcaaaaagtt
    83041 agatttgata atcgcggatg gacgaaattg cttgatacac ccgcttatca gttttacatg
    83101 gaagctctga tgcattgagt ctggacagtt ttgtcggctg gatacggcgt ttacgcggca
    83161 tccggcaaga acacatggtt ctttgcaaac aatcccatct ttctaccctg gaataatcgt
    83221 ttatatccct tggcattacc tctctttgtt tacattccaa catcatttta taaacattcc
    83281 gcttgtattt ttctttaccg taatgataat cgctatcact gcgatttact tttctttgca
    83341 tagattgact cagaaaaacg tttaagggtg ggtggcatgt ttgttccgtt tctcattatg
    83401 ttgcgcgaag gacttgaagc cgcgctgatt gtcagtttga ttgccagcta tcttaagcgt
    83461 acccagcgag gccgatggat tggtgtgatg tggattggcg tgttgcttgc cgctgcgttg
    83521 tgcctgggcc tgggtatctt cattaacgaa accaccggcg aatttccgca aaaagaacag
    83581 gaactgtttg aaggtatcgt ggcggtgatc gccgtggtga tccttacctg gatggttttc
    83641 tggatgcgca aagtgtcgcg caacgtcaaa gtgcaactgg aacaggcagt cgatagcgca
    83701 ttgcagcgtg gaaatcatca tggctgggcg ctggtgatga tggtcttttt tgccgttgca
    83761 agggaagggc tggagtcggt ctttttcctg ctggcggcat ttcaacaaga tgtcgggatc
    83821 tggccgccgc tgggtgcaat gctcggtctt gctactgccg tggtgctcgg cttcctgctc
    83881 tactggggcg gtattcgcct caatcttggt gcatttttta aatggaccag cctgtttatt
    83941 ctcttcgtcg ccgcagggct ggcggctggt gccattcgcg catttcatga agccggattg
    84001 tggaaccact ttcaggaaat cgccttcgat atgagcgcgg tcctctcaac tcactcgctg
    84061 tttggcacgc tgatggaagg gatttttggc tatcaggaag caccgagcgt cagcgaagtc
    84121 gccgtctggt ttatttatct catcccggcg ctggtggcat ttgccctgcc accacgcgca
    84181 ggggcgacag cgtctcgctc cgcgtagcaa atacgacgca aactcttgct tagttacaac
    84241 atactttaaa gggatagtct cgtcatgacc attaacttcc gccgtaacgc attgcagttg
    84301 agcgtggctg cgctgttctc ttctgctttt atggctaacg ccgctaatgt gccgcaggtc
    84361 aaggtgaccg tgaccgataa gcagtgcgaa ccgatgacca ttacggttaa cgccgggaaa
    84421 acacagttca ttattcagaa ccacagccag aaggcgctgg agtgggagat cctcaaaggc
    84481 gtgatggtgg tggaagagcg ggaaaatatc gcccctggct ttagccagaa aatgacggcg
    84541 aatttacagc ctggcgaata cgatatgacc tgcggtctgc tgactaaccc gaaagggaag
    84601 ttgatcgtca aaggtgaggc aacggcggat gcggcgcaaa gtgatgcgct gttaagtctt
    84661 ggtggtgcaa ttactgcata taaagcgtat gtcatggcgg aaaccacgca gctggtgacc
    84721 gacacgaaag cctttactga cgctattaaa gcaggcgata tcgaaaaagc gaaagcactg
    84781 tatgcgccga cgcgccagca ctatgagcgc atcgaaccga ttgctgaact gttctccgat
    84841 ctggatggca gcattgacgc ccgtgaagat gattacgagc aaaaagccgc cgatccgaaa
    84901 ttcaccggtt tccaccgtct ggaaaaagca ttgtttggcg acaacaccac caaaggcatg
    84961 gatcagtacg ctgaccagct ttataccgat gtggtcgatt tgcaaaaacg catcagtgaa
    85021 ctggctttcc caccttcaaa agtggtcggc ggtgcagccg gactgattga ggaagtggca
    85081 gccagcaaaa tcagcggtga agaagatcgc tacagccaca ccgatctgtg ggatttccag
    85141 gctaacgttg aaggctcgca gaaaattgtc gatctgctgc gtccacaact gcaaaaagcc
    85201 aacccggaac tgttggcaaa agtcgatgcc aactttaaaa aggtcgatac cattctggcg
    85261 aaataccgta ctaaagacgg ttttgaaacc tacgacaaat tgaccgatgc cgaccggaat
    85321 gcactgaaag gaccgattac tgcgctggcg gaagatctgg cgcaacttcg cggtgtgctg
    85381 ggattggatt aagcattatg cagtatgaag ataaaaacgg cgtgaatgaa ccgtcacgcc
    85441 gacgtttact gaaaggcatt ggtgcgctgg cgctggcggg aagttgtccg gtcgctcatg
    85501 cacaaaaaac gcaaagtgcg ccgggtacgc tttcaccgga tgcacgcaat gagaaacagc
    85561 cgttttatgg tgagcatcag gcagggatcc tgacgccaca acaggccgca atgatgctgg
    85621 tggcgtttga tgtgcttgcc agcgataaac ccgatcttga acggttgttt cgcttgttga
    85681 ctcagcgttt tgcttttctg actcagggcg gagcggcacc agaaacgcca aatccgcgcc
    85741 tgccaccact cgattccggc attcttggcg gctacattgc gcccgataat ctcaccatca
    85801 cgttatcggt gggtcactca ttgtttgatg agcgctttgg ccttgcgcca cagatgccga
    85861 aaaagttgca gaagatgacg cgttttccca acgactcgct ggatgcggcg ttatgtcatg
    85921 gtgatgtgtt gctacaaatt tgcgccaaca cccaggacac ggtgatccat gcgctgcgcg
    85981 atatcatcaa acatacgcca gatttgctca gcgtgcgctg gaagcgggaa gggtttattt
    86041 ccgatcacgc ggcgcgtagt aaaggcaaag agacgccgat taatttgctg ggtttcaaag
    86101 acggcactgc caatcccgat agccagaatg ataagttgat gcaaaaagtg gtgtgggtaa
    86161 cggcagatca gcaggagcca gcgtggacaa tcggtggcag ctatcaggca gtacgcttga
    86221 ttcagtttcg agtggaattt tgggacagaa ctccgctgaa agaacagcag acgatttttg
    86281 gtcgtgataa gcaaaccggt gcgccgctgg gaatgcagca tgagcatgat gttcccgatt
    86341 acgccagcga tccggaaggg aaggtgattg cgctggacag ccatatccgg ctggcgaatc
    86401 cccgcacggc ggagagtgag tccagcctga tgctgcgtcg tggctacagt tattcactgg
    86461 gcgtcaccaa ctccgggcaa ctggatatgg ggctactgtt tgtctgttac caacacgatc
    86521 tggaaaaagg cttcctgact gtacaaaaaa ggctcaatgg cgaagcgctg gaggaatacg
    86581 ttaaacctat cggcggcggt tatttttttg cgctgccggg agtgaaggac gcgaacgatt
    86641 atctcggaag cgcgttattg cgggtttaat gtttatagac ggataaggcg ttcttgcgca
    86701 gatgcctgat gcgatgcttg cgcgtcttct tatgcctaca atcggtgcgg gttcggtagg
    86761 cggataaggc gttcacgccg catccggcgt tcgtgcgcag atgcctgatg cgaatcctgc
    86821 tgaaagcaca cagctttttt catatctgtc atcactctgt catctttcca gtagaaacta
    86881 atgttactga aatggtgttt tatggttaaa tataagtgaa tatattgttg caatgaatgc
    86941 gagatctgtt gtaattatta agtagcagcg gaagttcccg gcagtgatag tcagtcacta
    87001 tggagatcgc ggatggtaac gtcctgttct ggacaatgtc ttgttaagct acgctccacc
    87061 acttgcggag atttctcctc cggtagcaac ttcgtcactc tgtatttttc tcacttcctc
    87121 tctttactcg tgttaccgac gccgcaaacg gtgtgcgtag ccgtttttcg gcattttatc
    87181 caaaagcaaa ctatggctta caaggaagcc aaccctctga tgttcgtgcg cataatcgcg
    87241 ctgccaacgg cgcgtgtgat gaatacaaac aactcaaggt gctctccatg ggaagacaaa
    87301 aagcagtgat caaagctcgt cgtgaggcaa aacgtgtgct gagacgggat tcgcgcagcc
    87361 ataagcagcg tgaagaagaa tcggtcacct cgcttgtgca gatgggcggc gtagaagcca
    87421 ttggtatggc ccgcgacagt cgcgatactt cgcccatcct cgcgcgaaat gaagcgcaat
    87481 tgcactatct gaaggctatt gagagtaagc agctgatatt cgccacgggc gaagccgggt
    87541 gcggaaaaac ctggatcagc gcagcaaaag cggcagaggc cctgatacat aaggatgtcg
    87601 acaggattat cgtcacccgt ccagttctgc aagccgatga agatcttggc ttcttacctg
    87661 gagatatcgc agaaaagttt gctccctatt ttcgcccggt ctacgacgtg ctggtccggc
    87721 gcttaggggc ttcctttatg cagtactgcc tgcgaccgga aatagggaag gtggaaattg
    87781 cgccgttcgc ctatatgcgt ggacgtacct ttgaaaatgc agtcgtcatt cttgacgagg
    87841 cgcagaatgt gactgccgcg caaatgaaaa tgtttttaac ccgcctcggg gagaacgtga
    87901 cggttatcgt caacggtgat atcacgcaat gcgatttgcc tcgcggcgtg tgctccggat
    87961 taagtgacgc gctggaacgt ttcgaagaag atgaaatggt tgggatcgtc cggttcggta
    88021 aagaggactg cgtacgttcg gcactttgcc aacgtacgct gcatgcatac agttaagtgt
    88081 attatcggtg cagagcccgg gcgaaccggg ctttgttttg ggtgtttatg cccggactag
    88141 cgctttttct gaaacaacca tttttatttg cccctggctg gtgaagtgta cgctcatcct
    88201 gtggcttttt tgtagttgct gatagagctc atcaggtatt gctaagctct ctgcatattc
    88261 ttgcggcgta tattggtagg ctgcatgatg ctgctgtttt tgaaaacgca gcttattgta
    88321 cagcgcccag acaattaaca cgacggcatt cgccactgcc agcaaaaaat agaactgaag
    88381 tcggcttctg gcctcgcttt gccagtaata acccgtcagc agatccatgg cgaataagaa
    88441 tatgaacaac gcaaataatg tccacaagat ggttgtggca acataatcaa ccagtaaacg
    88501 tactggtgat tgtcgggtcg taataattaa attgttcata ttaacctctc agaatcccgc
    88561 gatcgggact tacccaacgg gcgcgttgct ttttaggcat caacatgact cgtgtaaatg
    88621 ataccaatgt cgttgccagg ctcagcatcc agaaaataac cgggaaccaa ataatccaga
    88681 aaagcgatga agtcagatta tgctcatagc gattctcgat catcaggctg acaataaatt
    88741 gcagtaaaca taacgtacac aataatattc cggcagtatg tgtcgcagcg atatgtgtca
    88801 attcaatatt taacggtaca ccggcaagtt ggactgcgta aataatgaaa ccgaccaggc
    88861 aggtgaaggc ccatattgtc gtcaggcagt attcaaaaaa cagcggccac attcgaaagt
    88921 tttctttgcg ccacaacctt gtcatatttt tgaggaatac ttctgcaccg ccctgagccc
    88981 agcgcaggcg ctgtttccac agccctttta acgtttcagg cattaatatc cagcacagcg
    89041 cccgtggctc gtaaaaaatc gtccactgat tcaactgcag cttccagcta atatcaatat
    89101 cttcggtgat catatcgtca ctccagtaac ccacttctgc cagggcgctg cgacgaaatg
    89161 cggcaataac accggaaacg gtaaatacgt ttccatagat acgctgggtt cgcttgatca
    89221 aaccaataat tgaggaatac tcgccaacct gaattttacc caccagggta gaacgtgttc
    89281 gaatacgagg attaccggtt acggcaccca cacgcgggtt gtacaacatc ggttccacaa
    89341 tatatgccgc cgcatcgcgg tctaataacg catcgccatc aatgcacacc agatattcac
    89401 ttttcgccgc ggcagctccg gttttaagcg caatggcttt cccctggttt tgcgccagat
    89461 gaatgacccg caaatgggga atttgtgcag ccatgcgatc caggatggca cgggttttat
    89521 ctgttgaacc gtcatttacg gcaataactt caatgttctc ataacgctgt gctaaagcgg
    89581 cgtgtatggt ttcctcaacg tttttctcct cattaaaaca gggaatgata atggagatag
    89641 acggattatc tttcaactgg ggagctggtg cgttttctcc ccacggccag tggcgttcac
    89701 gatagaccca gaaatagacg ccgccaacaa tccacataat ggacataaaa aacggccaga
    89761 agaaaacgaa cctcatcatc agttcaccag agtgaaagta cgctacgcat aggggtatgc
    89821 ataacaccag acataatatt ttcggatacc aggctgttga aaactcagga cgaataaggt
    89881 ctatttcagg ttggttatgc agaaaattgt cgggataata accatagttt ttcactccat
    89941 tcagttgtaa taggctcatc cagtgagcga gttgttgcga agaaatagcc tgatgctgac
    90001 catttttctg ccagttttgt gcctgtaatt ctaaaataga tttgtcttta gcctgaggga
    90061 tgtttttaat ttgattggtc aattgtatta accattggtc agccgatttt tctgcgacac
    90121 cttccagata aggcatagcc ataatagcgg tccagtcata gctttttagg aaatcagcat
    90181 aattctgtgc aaaccaggct tcactttcag gttgtattac cggaagtgca aaaatatttc
    90241 gtgcagtttt aatatgtgga ccgcgaatgg cttttacgcg cgcactaagt tctaaagtga
    90301 agtcagttaa cgcacgactt ttaaagcggg cccactgttt aaattgctcc gggttttgtc
    90361 gaatttcgct cagactcccg ctaaagcctg cttgctgata agccgtgata gccggtgcac
    90421 tggcatcttc ataatctgaa agcaaagcat catcgtggaa caatatgcca tcaaaagcag
    90481 catgtccggc aagatcttca tataacatgc caacttgtgc tctgactctg tcatcgaaag
    90541 gagagagacg gtgatattgt tcaggatgaa tttgtgcttt tttctcccct gttggtaagt
    90601 attttactcg cgttaatgtg ggatctaaat cccagcttaa taccggcatc cacgcataga
    90661 tgtttacacc tgagcgggta cgtaattgcc aggcaacccg actaaaaata tctgctttca
    90721 ttggtagcaa acgatttgga aaccagacct ctttgaccag cccatcacca tcgggatcag
    90781 caaatgcctg caaatacacg gttgatattt gcatatcttt cacccgctga attagcacat
    90841 caatattgcg atccatttgc tggaggtttt cgtcataaac gtaatcaaga tcgatatgca
    90901 ttatccgttg tggtgatttt tcctgtacgg taataatttg ctgggcaaac tcttttaatg
    90961 agggattatt ggcgattaat acccgcggaa tggaatccaa ttgcgacgca tttgccaaac
    91021 ctgattcaag ggtgaagaac atgtcataac cgagtttttt taattcctct atcgctatgc
    91081 cattcgcttc gccataaggc caaacaaaaa cgtgtggatt tacctcaacc tttgtacgca
    91141 ggtattccgt catttttaca gcatccagac gaattctttc ccggtattct gctgcggttt
    91201 cataccgtgc gtggtcagta aaatatgcac gatttacata tacaggcaat aagctgccgg
    91261 tggcattagc ctgaataccg tagtgagaat tccatgtatg agaagcgagc tcaacgagcc
    91321 gggaacgcgc aacttctcgc acttgttgcc acgtggcaaa atattctcga tcgaccaact
    91381 catcgccaaa ttttacttgt ttatccgctg gcgtatcgac ccaactgccg acgggggccc
    91441 atacagcagg ccactggaag gcctgaagaa ttgggaagac gcgggtataa aaactctggt
    91501 agccgtcatc aaaagtcagc actacagctt tttccggtag cggttttcct cctcgatgtg
    91561 cttcacgaat ttgagcaata ctgaccggtt gataaccgtt ctcgcgcagc caggcaaatt
    91621 gttcacgcag tgctgatgtc cgcactgaca taaaacgctg gtcggcagct tcgtcttcaa
    91681 cgttatgcca tgaaatcgct acaaaaccat tatgcggcca cggttgctcg gcgagtaaag
    91741 attcgcgatc ctgtggcggt ataaatgatg ttcttgactg gctaatgcac gcggtgagca
    91801 taattatact caccagcatc aggagatatt tatttccatt acgtaacata tttatcctta
    91861 aaatctgaat gtcatatcga attcaacgta taagttgtgt tctctgtcac cgtcataagg
    91921 tcgtttttcc cagcgtagcg ttgcgccagc atcaatcacg tcattccagc taatgcgttg
    91981 cccgtagccg agttgggtga cgacatccgt gccataatgt ttttgccagg aggcaccaac
    92041 acctgcgctg aatatttgct cccagctatt ttcatagctt cgccataaca aatggcttgc
    92101 ctcaaatgcc ggaacaatat cgaacgtttt tatagggttg tagtatgggg tatcgtgttc
    92161 tgtattttgt tcgtaataca gactgggtag gaaatcgaca atcaaatatg gtgaagacca
    92221 gatgcgttcc tgaccctcaa gtgagacttc atgacgctgg ttactgtcgg aaaaatcagt
    92281 gaaagcccag gagacaccgt acttacgccg ctcattttga taccagcgaa cataagcctg
    92341 agcactgttg cctgtaacac catttttcat tgcccgtaat ggaacgcggt gagagaggcg
    92401 ttccagttgc gaaccaatac gccagttatc attaaaatca taccagccag acaggcgcgc
    92461 gccgggttta tgctcatgat tgaaaacgcg ttcagcgtac tctgcctcga gccagatatt
    92521 acgtgaccgc cactcaacac ccgcaagcca gtcgcgaaca atcccttttc cttcgctaaa
    92581 ttgtccatcg gcataaccga atccagcaaa accgcgccag ttatccttca gcggtggtga
    92641 ataaacgatg gtggttaagt cgacatcatg tttaccacta tccgggcctt cggcatcaat
    92701 tcctgttgag ccagcgatac gaagctctgc aagattatgt acatcaaccg cacgttttaa
    92761 tcgtacaacg ccgggatctt gcggttcacg ttcgacaaca tcgtgcgtta agacagctgc
    92821 ctgctgccat tcttgtaacg ttaatgctgt ccaggcttgt tcaacctcca gattaatatt
    92881 acgtggctcg atcacttctg ctttttttaa ttcattttct gctgcacgag gccaaccgcg
    92941 ggcttgtaac acactcgcgt aatcaatgcg cagtccctga tttcctggtg cgttataagc
    93001 aagctctctg gctgtcattt cagcctgagg aagatcatta ctatattttg ctacggttga
    93061 gagaaacgaa tgcccctgta accaggtatc attcgggatg ctcgtaggcg tgcccattaa
    93121 ccgaaggaaa ggcggcgaag tattaatggt atgttgggtg acagttagcg cgcccggata
    93181 attttcactc tccaggtggc tgtaaaagag atccgcaagt tcttcatcgg ataaatccgg
    93241 ggcaatggtc tccttgtgat aaaagagctc ggtcattatt gactgtgctt ttttcggctg
    93301 atgatctttg agataagccg atgcaaccca atattgcccc cagggcggaa taatttgccc
    93361 cgtctttttt aatcgctgat agtgagaaat aacgtcttta taacgatcgc gagttaataa
    93421 cgcgccaaga tgatcaacct gaatacgctg gtactgggca gtacggtctg ggttatcgtg
    93481 ccacagaatt tctaatgcag cgtattgggc gagggcgcga tcggcaatgg cataacgttc
    93541 actttcactg cgcgtaggca taaacgacag tctgaccagt tcggcatgaa tatcagcgcg
    93601 aatatctggc gttaaattgg catcgtcaat cgcggcagca agttgattat tacgtaatgc
    93661 ctgcacgtat tctgtgggat attgttgcgt agatgcattt tcaggtaatg actctgtcat
    93721 cgcccgtaat tcatcctgat gacgccccgc cagtttatag atataggctt ctgcgagtaa
    93781 attggctttg tccggtgctc cagagttaag ctgcttaagt ttaaccagcg cagtatcata
    93841 gtgaccagca tctgccaggg ttaaaatttg tccccgttga taatccttat tttgcggctc
    93901 cagagagagc gccttttgcc acagtgtaag cgagttttgc cattgttgca ggttacgata
    93961 agcgacggcg acagctgcat aaccacgcgc tggtaattgc tgatgacggt agcggttgta
    94021 aacggtaata acctgtttat cttgcccggc ccataaggca atctgtaacc agtcagcaat
    94081 ttggttattg ctgagtgctg attttagtgc aaaccatgac aaagctggct gagtattacc
    94141 cttgcgagct ttaataatca atgcatcata ggcgttatta acagcactct tcgccgcggg
    94201 actcgctgct aaaaatgcgg cagtaagaag tttcaaagcc catttggttt tcgggcacct
    94261 ttttctgcta cttgaataca tcctgtatta ctccatgtat tgccaaaatc tctctctgta
    94321 tctaattaca ggtaactgaa aagaaagata tttttgcacc tcataatccg ttattaaacg
    94381 cggaagagag acgtgaattg ttgatgatga gaagaagaaa tgatgagcag agtgtccata
    94441 taaaatcctt ttctcgcccg aaaatccatt ccaatgatga ggatcttcag gaatacggc
//
LOCUS       DAZGXZ010000005        80119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000005 DAZGXZ010000000
VERSION     DAZGXZ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.005"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..568
                     /gene="yraH"
                     /locus_tag="ACHKJF_000415"
     CDS             <1..568
                     /gene="yraH"
                     /locus_tag="ACHKJF_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0947838.1"
                     /translation="TAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSDKKIEVA
                     LGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPN
                     FPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGIVNVDLI
                     AYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            648..1343
                     /gene="yraI"
                     /locus_tag="ACHKJF_000416"
     CDS             648..1343
                     /gene="yraI"
                     /locus_tag="ACHKJF_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0947839.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1373..3889
                     /gene="yraJ"
                     /locus_tag="ACHKJF_000417"
     CDS             1373..3889
                     /gene="yraJ"
                     /locus_tag="ACHKJF_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0947840.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3900..4991
                     /gene="yraK"
                     /locus_tag="ACHKJF_000418"
     CDS             3900..4991
                     /gene="yraK"
                     /locus_tag="ACHKJF_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0947841.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GTNTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFRNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(5034..5894)
                     /gene="rsmI"
                     /locus_tag="ACHKJF_000419"
     CDS             complement(5034..5894)
                     /gene="rsmI"
                     /locus_tag="ACHKJF_000419"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP0947842.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            5958..7994
                     /gene="lpoA"
                     /locus_tag="ACHKJF_000420"
     CDS             5958..7994
                     /gene="lpoA"
                     /locus_tag="ACHKJF_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP0947843.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            7952..8347
                     /gene="yraN"
                     /locus_tag="ACHKJF_000421"
     CDS             7952..8347
                     /gene="yraN"
                     /locus_tag="ACHKJF_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP0947844.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8367..8957
                     /gene="diaA"
                     /locus_tag="ACHKJF_000422"
     CDS             8367..8957
                     /gene="diaA"
                     /locus_tag="ACHKJF_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP0947845.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            8967..9542
                     /gene="dolP"
                     /locus_tag="ACHKJF_000423"
     CDS             8967..9542
                     /gene="dolP"
                     /locus_tag="ACHKJF_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0947846.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9656..10696)
                     /gene="yraQ"
                     /locus_tag="ACHKJF_000424"
     CDS             complement(9656..10696)
                     /gene="yraQ"
                     /locus_tag="ACHKJF_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0947847.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGTVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10769..11404)
                     /gene="yraR"
                     /locus_tag="ACHKJF_000425"
     CDS             complement(10769..11404)
                     /gene="yraR"
                     /locus_tag="ACHKJF_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0947848.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11532..12050
                     /gene="yhbO"
                     /locus_tag="ACHKJF_000426"
     CDS             11532..12050
                     /gene="yhbO"
                     /locus_tag="ACHKJF_000426"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP0947849.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12030..12473)
                     /gene="yhbP"
                     /locus_tag="ACHKJF_000427"
     CDS             complement(12030..12473)
                     /gene="yhbP"
                     /locus_tag="ACHKJF_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP0947850.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12524..12826
                     /gene="yhbQ"
                     /locus_tag="ACHKJF_000428"
     CDS             12524..12826
                     /gene="yhbQ"
                     /locus_tag="ACHKJF_000428"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP0947851.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12813..13316)
                     /gene="yhbS"
                     /locus_tag="ACHKJF_000429"
     CDS             complement(12813..13316)
                     /gene="yhbS"
                     /locus_tag="ACHKJF_000429"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0947852.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13310..13834)
                     /gene="ubiT"
                     /locus_tag="ACHKJF_000430"
     CDS             complement(13310..13834)
                     /gene="ubiT"
                     /locus_tag="ACHKJF_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP0947853.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14043..15038
                     /gene="ubiU"
                     /locus_tag="ACHKJF_000431"
     CDS             14043..15038
                     /gene="ubiU"
                     /locus_tag="ACHKJF_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP0947854.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15047..15925
                     /gene="ubiV"
                     /locus_tag="ACHKJF_000432"
     CDS             15047..15925
                     /gene="ubiV"
                     /locus_tag="ACHKJF_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP0947855.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16131..17138
                     /gene="yhbW"
                     /locus_tag="ACHKJF_000433"
     CDS             16131..17138
                     /gene="yhbW"
                     /locus_tag="ACHKJF_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP0947856.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17256..18500)
                     /gene="mtr"
                     /locus_tag="ACHKJF_000434"
     CDS             complement(17256..18500)
                     /gene="mtr"
                     /locus_tag="ACHKJF_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP0947857.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVLMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18654..20543)
                     /gene="deaD"
                     /locus_tag="ACHKJF_000435"
     CDS             complement(18654..20543)
                     /gene="deaD"
                     /locus_tag="ACHKJF_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP0947858.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20536..20616)
                     /gene="yrbN"
                     /locus_tag="ACHKJF_000436"
     CDS             complement(20536..20616)
                     /gene="yrbN"
                     /locus_tag="ACHKJF_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP0947859.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20723..21607)
                     /gene="nlpI"
                     /locus_tag="ACHKJF_000437"
     CDS             complement(20723..21607)
                     /gene="nlpI"
                     /locus_tag="ACHKJF_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP0947860.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21716..23851)
                     /gene="pnp"
                     /locus_tag="ACHKJF_000438"
     CDS             complement(21716..23851)
                     /gene="pnp"
                     /locus_tag="ACHKJF_000438"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP0947861.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24098..24367)
                     /gene="rpsO"
                     /locus_tag="ACHKJF_000439"
     CDS             complement(24098..24367)
                     /gene="rpsO"
                     /locus_tag="ACHKJF_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP0947862.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24516..25460)
                     /gene="truB"
                     /locus_tag="ACHKJF_000440"
     CDS             complement(24516..25460)
                     /gene="truB"
                     /locus_tag="ACHKJF_000440"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP0947863.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25460..25861)
                     /gene="rbfA"
                     /locus_tag="ACHKJF_000441"
     CDS             complement(25460..25861)
                     /gene="rbfA"
                     /locus_tag="ACHKJF_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP0947864.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26025..28697)
                     /gene="infB"
                     /locus_tag="ACHKJF_000442"
     CDS             complement(26025..28697)
                     /gene="infB"
                     /locus_tag="ACHKJF_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP0947865.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28722..30209)
                     /gene="nusA"
                     /locus_tag="ACHKJF_000443"
     CDS             complement(28722..30209)
                     /gene="nusA"
                     /locus_tag="ACHKJF_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP0947866.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30237..30689)
                     /gene="rimP"
                     /locus_tag="ACHKJF_000444"
     CDS             complement(30237..30689)
                     /gene="rimP"
                     /locus_tag="ACHKJF_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP0947867.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30896..30972)
                     /locus_tag="ACHKJF_000445"
     tRNA            complement(30896..30972)
                     /locus_tag="ACHKJF_000445"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30936..30938),aa:Met,seq:cat)
     gene            31320..32663
                     /gene="argG"
                     /locus_tag="ACHKJF_000446"
     CDS             31320..32663
                     /gene="argG"
                     /locus_tag="ACHKJF_000446"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP0947868.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(32671..34296)
                     /gene="yhbX"
                     /locus_tag="ACHKJF_000447"
     CDS             complement(32671..34296)
                     /gene="yhbX"
                     /locus_tag="ACHKJF_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0947869.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34755..34841)
                     /locus_tag="ACHKJF_000448"
     tRNA            complement(34755..34841)
                     /locus_tag="ACHKJF_000448"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34805..34807),aa:Leu,seq:gag)
     gene            complement(34856..35188)
                     /gene="secG"
                     /locus_tag="ACHKJF_000449"
     CDS             complement(34856..35188)
                     /gene="secG"
                     /locus_tag="ACHKJF_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP0947870.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35416..36753)
                     /gene="glmM"
                     /locus_tag="ACHKJF_000450"
     CDS             complement(35416..36753)
                     /gene="glmM"
                     /locus_tag="ACHKJF_000450"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP0947871.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMSRNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36746..37594)
                     /gene="folP"
                     /locus_tag="ACHKJF_000451"
     CDS             complement(36746..37594)
                     /gene="folP"
                     /locus_tag="ACHKJF_000451"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP0947872.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37684..39618)
                     /gene="ftsH"
                     /locus_tag="ACHKJF_000452"
     CDS             complement(37684..39618)
                     /gene="ftsH"
                     /locus_tag="ACHKJF_000452"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP0947873.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39718..40347)
                     /gene="rlmE"
                     /locus_tag="ACHKJF_000453"
     CDS             complement(39718..40347)
                     /gene="rlmE"
                     /locus_tag="ACHKJF_000453"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP0947874.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40473..40766
                     /gene="yhbY"
                     /locus_tag="ACHKJF_000454"
     CDS             40473..40766
                     /gene="yhbY"
                     /locus_tag="ACHKJF_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP0947875.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40922..41398)
                     /gene="greA"
                     /locus_tag="ACHKJF_000455"
     CDS             complement(40922..41398)
                     /gene="greA"
                     /locus_tag="ACHKJF_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP0947876.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41646..43079
                     /gene="dacB"
                     /locus_tag="ACHKJF_000456"
     CDS             41646..43079
                     /gene="dacB"
                     /locus_tag="ACHKJF_000456"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0947877.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43119..44291)
                     /gene="cgtA"
                     /locus_tag="ACHKJF_000457"
     CDS             complement(43119..44291)
                     /gene="cgtA"
                     /locus_tag="ACHKJF_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP0947878.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44307..45272)
                     /gene="yhbE"
                     /locus_tag="ACHKJF_000458"
     CDS             complement(44307..45272)
                     /gene="yhbE"
                     /locus_tag="ACHKJF_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0947879.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45399..45656)
                     /gene="rpmA"
                     /locus_tag="ACHKJF_000459"
     CDS             complement(45399..45656)
                     /gene="rpmA"
                     /locus_tag="ACHKJF_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP0947880.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45677..45988)
                     /gene="rplU"
                     /locus_tag="ACHKJF_000460"
     CDS             complement(45677..45988)
                     /gene="rplU"
                     /locus_tag="ACHKJF_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP0947881.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46247..47218
                     /gene="ispB"
                     /locus_tag="ACHKJF_000461"
     CDS             46247..47218
                     /gene="ispB"
                     /locus_tag="ACHKJF_000461"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP0947882.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47446..47724
                     /gene="sfsB"
                     /locus_tag="ACHKJF_000462"
     CDS             47446..47724
                     /gene="sfsB"
                     /locus_tag="ACHKJF_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP0947883.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47772..49031)
                     /gene="murA"
                     /locus_tag="ACHKJF_000463"
     CDS             complement(47772..49031)
                     /gene="murA"
                     /locus_tag="ACHKJF_000463"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP0947884.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49086..49340)
                     /gene="ibaG"
                     /locus_tag="ACHKJF_000464"
     CDS             complement(49086..49340)
                     /gene="ibaG"
                     /locus_tag="ACHKJF_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP0947885.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49500..49793)
                     /gene="mlaB"
                     /locus_tag="ACHKJF_000465"
     CDS             complement(49500..49793)
                     /gene="mlaB"
                     /locus_tag="ACHKJF_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP0947886.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49793..50428)
                     /gene="mlaC"
                     /locus_tag="ACHKJF_000466"
     CDS             complement(49793..50428)
                     /gene="mlaC"
                     /locus_tag="ACHKJF_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP0947887.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50447..50998)
                     /gene="mlaD"
                     /locus_tag="ACHKJF_000467"
     CDS             complement(50447..50998)
                     /gene="mlaD"
                     /locus_tag="ACHKJF_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP0947888.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51003..51785)
                     /gene="mlaE"
                     /locus_tag="ACHKJF_000468"
     CDS             complement(51003..51785)
                     /gene="mlaE"
                     /locus_tag="ACHKJF_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP0947889.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51793..52602)
                     /gene="mlaF"
                     /locus_tag="ACHKJF_000469"
     CDS             complement(51793..52602)
                     /gene="mlaF"
                     /locus_tag="ACHKJF_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP0947890.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52812..53789
                     /gene="yrbG"
                     /locus_tag="ACHKJF_000470"
     CDS             52812..53789
                     /gene="yrbG"
                     /locus_tag="ACHKJF_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP0947891.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGGRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53803..54789
                     /gene="kdsD"
                     /locus_tag="ACHKJF_000471"
     CDS             53803..54789
                     /gene="kdsD"
                     /locus_tag="ACHKJF_000471"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP0947892.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54810..55376
                     /gene="kdsC"
                     /locus_tag="ACHKJF_000472"
     CDS             54810..55376
                     /gene="kdsC"
                     /locus_tag="ACHKJF_000472"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP0947893.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55373..55948
                     /gene="lptC"
                     /locus_tag="ACHKJF_000473"
     CDS             55373..55948
                     /gene="lptC"
                     /locus_tag="ACHKJF_000473"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP0947894.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55917..56474
                     /gene="lptA"
                     /locus_tag="ACHKJF_000474"
     CDS             55917..56474
                     /gene="lptA"
                     /locus_tag="ACHKJF_000474"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP0947895.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56481..57206
                     /gene="lptB"
                     /locus_tag="ACHKJF_000475"
     CDS             56481..57206
                     /gene="lptB"
                     /locus_tag="ACHKJF_000475"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP0947896.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57254..58687
                     /gene="rpoN"
                     /locus_tag="ACHKJF_000476"
     CDS             57254..58687
                     /gene="rpoN"
                     /locus_tag="ACHKJF_000476"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP0947897.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58710..58997
                     /gene="hpf"
                     /locus_tag="ACHKJF_000477"
     CDS             58710..58997
                     /gene="hpf"
                     /locus_tag="ACHKJF_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP0947898.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59115..59606
                     /gene="ptsN"
                     /locus_tag="ACHKJF_000478"
     CDS             59115..59606
                     /gene="ptsN"
                     /locus_tag="ACHKJF_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP0947899.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59652..60506
                     /gene="rapZ"
                     /locus_tag="ACHKJF_000479"
     CDS             59652..60506
                     /gene="rapZ"
                     /locus_tag="ACHKJF_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP0947900.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60503..60775
                     /gene="npr"
                     /locus_tag="ACHKJF_000480"
     CDS             60503..60775
                     /gene="npr"
                     /locus_tag="ACHKJF_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP0947901.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60989..61621
                     /gene="yrbL"
                     /locus_tag="ACHKJF_000481"
     CDS             60989..61621
                     /gene="yrbL"
                     /locus_tag="ACHKJF_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP0947902.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61618..62346)
                     /gene="mtgA"
                     /locus_tag="ACHKJF_000482"
     CDS             complement(61618..62346)
                     /gene="mtgA"
                     /locus_tag="ACHKJF_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP0947903.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62343..62996)
                     /gene="elbB"
                     /locus_tag="ACHKJF_000483"
     CDS             complement(62343..62996)
                     /gene="elbB"
                     /locus_tag="ACHKJF_000483"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP0947904.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63226..65562)
                     /gene="arcB"
                     /locus_tag="ACHKJF_000484"
     CDS             complement(63226..65562)
                     /gene="arcB"
                     /locus_tag="ACHKJF_000484"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP0947905.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65658..66587)
                     /gene="yhcC"
                     /locus_tag="ACHKJF_000485"
     CDS             complement(65658..66587)
                     /gene="yhcC"
                     /locus_tag="ACHKJF_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP0947906.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67262..71722
                     /gene="gltB"
                     /locus_tag="ACHKJF_000486"
     CDS             67262..71722
                     /gene="gltB"
                     /locus_tag="ACHKJF_000486"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP0947907.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71735..73153
                     /gene="gltD"
                     /locus_tag="ACHKJF_000487"
     CDS             71735..73153
                     /gene="gltD"
                     /locus_tag="ACHKJF_000487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP0947908.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TNDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73337..74464
                     /gene="yhcG"
                     /locus_tag="ACHKJF_000488"
     CDS             73337..74464
                     /gene="yhcG"
                     /locus_tag="ACHKJF_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP0947909.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74524..74988)
                     /gene="nanQ"
                     /locus_tag="ACHKJF_000489"
     CDS             complement(74524..74988)
                     /gene="nanQ"
                     /locus_tag="ACHKJF_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP0947910.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74985..75860)
                     /gene="nanK"
                     /locus_tag="ACHKJF_000490"
     CDS             complement(74985..75860)
                     /gene="nanK"
                     /locus_tag="ACHKJF_000490"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0947911.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75857..76546)
                     /gene="nanE"
                     /locus_tag="ACHKJF_000491"
     CDS             complement(75857..76546)
                     /gene="nanE"
                     /locus_tag="ACHKJF_000491"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP0947912.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76594..78084)
                     /gene="nanT"
                     /locus_tag="ACHKJF_000492"
     CDS             complement(76594..78084)
                     /gene="nanT"
                     /locus_tag="ACHKJF_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP0947913.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78193..79086)
                     /gene="nanA"
                     /locus_tag="ACHKJF_000493"
     CDS             complement(78193..79086)
                     /gene="nanA"
                     /locus_tag="ACHKJF_000493"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0947914.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79208..79999)
                     /gene="nanR"
                     /locus_tag="ACHKJF_000494"
     CDS             complement(79208..79999)
                     /gene="nanR"
                     /locus_tag="ACHKJF_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0947915.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19726 a        21659 c        19891 g        18843 t
ORIGIN      
        1 aacagctatt gtgggcttac ttgcgctttt cgcaggtaat gccgctgcaa ccgatggtga
       61 aatagttttt gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa
      121 aattgaagtt gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag
      181 cccaaaaatt ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga
      241 taacggcaac gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt
      301 ccctaacttc cctaatctgg caaaagtggg ctcttttgct ggcacagcgg caacaggtgt
      361 gggtatccgt attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa
      421 tgataacacg gtttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat
      481 cgcttactac gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt
      541 cgttaacgta acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa
      601 cgcccttttc cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa
      661 cattcgcggt gataataacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg
      721 gtatcgtttt acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac
      781 ctgtcgcaaa caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag
      841 atggtacaag ccgtgcccca tttattataa ccccaccgct atttcgtctt gaagctggcg
      901 atgactcatc actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc
      961 tgttctacat taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta
     1021 acgagttgac gctggtattt aaaacacgga tcaaaatgtt ttatcgcccc gcacacctga
     1081 agggacgggt aaacgatgcg tggacatcac tggaatttaa acgtagtgac cattcactca
     1141 atatatataa cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg
     1201 atctcacaag caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttcctg
     1261 catctggcgg aaagaacgtg aaatgggctg caatcaatga ttatggcggc agttccggga
     1321 cagaaactcg tccactgcaa taacaaatat aaaaaacaca ggtcatcagg gaatgccaca
     1381 acgacaccac cagggacata aacgcacacc gaaacagttg gcgctcatca tcaaacgctg
     1441 tttgccgatg gtgctcactg gcagcggcat gctttgcact accgctaacg ccgaagagta
     1501 ttatttcgac cccattatgc tggaaactac aaaaagtggt atgcaaacaa ctgatctgtc
     1561 acgtttttca aaaaaatacg cacaactacc aggaacttat caggttgata tctggctgaa
     1621 taaaaagaag gtttcacaga aaaaaattac atttaccgcc aatgcagagc aacttctgca
     1681 gccacagttt acggtagaac aactacgtga gctgggtatt aaggtggatg aaatcccggc
     1741 gctggctgaa aaagatgacg atagcgtgat caactcgctt gaacaaatca ttcccggtac
     1801 agctgctgaa tttgatttca atcatcagcg acttaatttg agcattcccc aaattgcact
     1861 gtaccgtgat gcaagaggtt acgtctcccc ttctcgttgg gacgatggta taccaacgct
     1921 gtttaccaac tactcgttta caggttctga taaccgttac cgccagggca atcgtagcca
     1981 acgacagtac ctgaatatgc aaaatggtgc taattttggc ccctggcgat tacgtaacta
     2041 ttctacgtgg acacgcaacg atcaggcgtc aagctggaac actatcagta gttatttaca
     2101 acgtgatatc aaggcgttga agtctcagtt gcttctggga gaaagcgcca ccagcggcag
     2161 tattttttcc agctacacct ttactggcgt gcaactcgct tccgacgata atatgttgcc
     2221 aaacagccag cgcggatttg ccccaacggt acgcggtatc gcaaacagta gtgcaatcgt
     2281 gactatcagg caaaatggtt atgtgatcta tcaaagcaac gtgccagcgg gtgcctttga
     2341 aattaacgat ctctacccct cttccaacag cggcgattta gaagtcacga ttgaagaaag
     2401 tgacggtacg caacgtcgct ttatccagcc ttattcttca ttacccatga tgcagcgacc
     2461 tgggcatcta aaatatagcg cgaccgctgg acgctatcgc gctgatgcaa acagtgatag
     2521 caaggaaccc gaatttgctg aagccacggc aatatatggt ttgaataata cttttacgct
     2581 gtatggcggc ctgctcggtt ctgaagatta ttatgcgctg gggatcggta tcggcggcac
     2641 acttggcgca ctgggcgcgt tgtcgatgga tatcaacaga gctgacaccc aattcgataa
     2701 ccggcactct tttcatggct atcaatggcg tacgcagtac atcaaagata tcccggaaac
     2761 caacaccaat atcgctgtta gctactatcg ctataccaac gatggctatt ttagttttga
     2821 tgaagccaat acccgcaatt gggactataa cagtcgccaa aaaagtgaaa ttcaattcaa
     2881 catcagccag acaatatttg atggggtaag tctgtatgcc tccggttcgc agcaagacta
     2941 ttggggcaat aacgataaaa acaggaatat ctcagttggg gtttccggcc agcaatgggg
     3001 aattggttac agcctgaatt atcaatacag tcgctacacg gatcaaaata atgaccgcgc
     3061 actctctttg aatctcagta ttccgttaga acgctggtta ccgcgtagcc gggtttccta
     3121 tcagatgacc agccagaaag atcgcccaac ccaacatgaa atgcgtcttg atggctcact
     3181 gctggatgat ggtcgcctga gctatagcct ggaacaaagt ctggatgacg ataacaacca
     3241 taacagtagc ctgaacgcca gttaccgttc accttatggc accttcagtg ccggatacag
     3301 ttacggtaat gacagcagcc aatacaatta cggcgttacc ggcggcgtgg ttatccatcc
     3361 tcatggcgtg acgctctcgc aatatctggg caacgctttt gcgcttatcg atgctaatgg
     3421 ggcttctggc gtgaggatac aaaactatcc ggggattgct actgatccct ttggctatgc
     3481 agtggttcct tatctcacga cttaccagga aaaccgtctc tcggtagata ctacgcagct
     3541 gcccgataac gtcgatcttg agcaaacaac acagtttgtg gtgcccaaca gaggtgcaat
     3601 ggtagcggcg cgtttcaacg ccaatatcgg ttatcgcgta cttgttacag tcagcgatcg
     3661 caacggtaaa ccgttgccct ttggcgctct tgccagcaac gatgagacgg ggcaacaaag
     3721 tatcgtcgat gagggcggca tactatatct ctctgggata tcgagtaaat cacaaagctg
     3781 gactgtacgc tggggaaatc aggcagatca acaatgtcag tttgctttta gtacaccgga
     3841 ttcagaacca acaacctctg tattacaagg cacggcgcag tgccattaag gataaaaaaa
     3901 tgaaaagagc gcctcttata acaggacttt tgttgatatc cacatcctgc gcttatgcct
     3961 cctcaggagg gtgtggagcc gacagcacta gcggtgcgac aaattacagc agtgtggttg
     4021 atgatgttac ggtgaaccag acagataacg tgacaggacg ggagtttacc tctgcaacgc
     4081 taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca gttaaacttg
     4141 tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat tacaaactca
     4201 atgacagcct ggatattaaa accacattac aggcaaacga cattccagga ctcacaaccg
     4261 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
     4321 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
     4381 gtacaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
     4441 cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga agcccgtcta
     4501 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
     4561 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
     4621 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
     4681 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
     4741 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
     4801 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcggaacg
     4861 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
     4921 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
     4981 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
     5041 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
     5101 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
     5161 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
     5221 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     5281 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     5341 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     5401 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     5461 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     5521 gcagcacacg gcccgggtag cggcaccaca cgaatccccg cttcacggca ggtacgcacc
     5581 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     5641 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     5701 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     5761 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgctcgctg ggtgatatcc
     5821 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     5881 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     5941 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     6001 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     6061 ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt cagcagatgc
     6121 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     6181 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     6241 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     6301 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     6361 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     6421 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaacag cagaatattg
     6481 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     6541 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     6601 gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt tatccgaaca
     6661 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     6721 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     6781 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     6841 tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc gtggatggcg
     6901 ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgaacagcct gcggcacagc
     6961 cagcgcctgt aagcaccccg gcagcaacca ccacagcggt aagcgcgcca gcgaatccat
     7021 ccgcagaact gaaaatctac gatacctcat cacaaccact tagccagatc ttaagccagg
     7081 ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac gttgaagagt
     7141 tgcttaagag caacaccccg ctgaacgtgc tggcactgaa ccagccggag aatatcgaaa
     7201 accgcgtcaa tatttgttac ttcgcgcttt caccggaaga cgaagcgcgc gatgcagcgc
     7261 gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc agttcattgg
     7321 gcgatcgcgt agccaatgcg tttgcgcagg agtggcagaa actgggcggc ggcaccgttc
     7381 tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc ggttctggta
     7441 tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc atgacgacca
     7501 acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat ggcggtcgtg
     7561 tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa ccgatgatcg
     7621 ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc cagctcccgc agtgcgcaag
     7681 ggaccgctgg cccggatttc cgactggaga tggaaggctt gcagtacagc gaaatcccga
     7741 tgctggcagg cggtaatctg ccgttaatgc agcaggcgct cagcgcggtg aacaacgatt
     7801 attcgctggc tcgcatgtat gcgatgggcg ttgatgcctg gtcgttggca aatcatttct
     7861 ctcaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc ctgacggcaa
     7921 acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa ggtcaggtag
     7981 tccccgccag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag cgcgtcgctg
     8041 gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg gcggcgagat
     8101 cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct atcgccgctc
     8161 tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca aattattaca
     8221 gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg attgccggtt
     8281 cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct ttaatgacca
     8341 ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc ttcactgaaa
     8401 gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc cgtgcagcca
     8461 tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt aatggaactt
     8521 ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc
     8581 ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg attgccaacg
     8641 atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat gcgggagatg
     8701 tattgttagc catttccacg cgtggcaaca gccgcgatat tgtaaaagcg gtagaagctg
     8761 ccgttacgcg tgatatgacc attgtggcat taaccggcta tgacggcggt gaacttgcag
     8821 gtttgttagg gccacaggat gtggaaatcc gcattccttc gcatcgtagt gctcgcattc
     8881 aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac acgcttttcc
     8941 ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca gtccttattt
     9001 ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct gctgtgggta
     9061 ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt accctggaag
     9121 tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaagcg cgcattaatg
     9181 taacggccta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct gaactttcgg
     9241 ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat aacgagattc
     9301 gtcagggcca accgattggt ctgggcgaag cgtctaacga tacgtggatc accaccaaag
     9361 tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg
     9421 aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa gcggcggcag
     9481 atattgccag ccgggtaagc ggcgtgaaac gggtaactac ggcgtttacg tttattaaat
     9541 agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgata
     9601 caaaccgtag gtcagataag gcgtttacgc cgcatccgac atgttttccc tcaaatcaga
     9661 acaacagcgc cagcccgccg acaatcacac cagacactgc caccatcgcc cccgtcagcc
     9721 ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa ctcaccgccg
     9781 ggagcgtcat caacaatgcc agcgccggag cagttcccat acctgccagc atcatcgttt
     9841 gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgcc accgccatcg
     9901 ccaccaccca catcaggctg ttatcgacag taccatcggc atgggggaat aaccagacgc
     9961 gagcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc cagaaaagcg
    10021 tccatagcgc cctgccccag cggctaaaaa aaccgccctg tgcttccggt atgtcaattt
    10081 cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca
    10141 gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag ccgaggacaa
    10201 agcccataaa caccagcgtc gccgggttta acaccggatt gcccatccag aatgccagcg
    10261 caccgcccat cgacacctgt tggcgacgca ttcccgccgc gaccggagcc gcacagcagg
    10321 tacacatcat gcccggcaac gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt
    10381 gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga
    10441 tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg taatccaacg
    10501 ccgcctgcca tgggttagca tccgcctgcg caaggataga tttaccgata ctgtgggttt
    10561 cggcagcagt aaacgctttg ccgtagtaag gttcccattt cacataccag agaccggcaa
    10621 tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc gcctgagatg
    10681 aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc gcgtgaatat
    10741 tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat tctgaagagc
    10801 ttaaaatcgt cacgccttca tgctcaggcc gcatcgactc tgccagcatg acccgcgcca
    10861 cgtctctcgc atcaatggat ttccagttac ctggtaacaa acggaacagc ggcgcaaaaa
    10921 gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa catcgacggg cgagcaatgg
    10981 tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg acgcggttat
    11041 agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata tgctgcgcgc
    11101 ccagccgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca tgaataaacg
    11161 cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg
    11221 gatcggtgac ctgcgccagc gcgtcagtca gttgcggatc atggggattg aacactcctg
    11281 gcatatctcc caacggacgt cgcgtcggtg cagcaatagc gttaactttc ggttcgttaa
    11341 tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc agtacctgac
    11401 tcatcttcgc tcctttacag aattgtctgc cttgcgctcc ccggctcatg caccaggctt
    11461 aatagaccat gaggtaatta tccccgattg tgagaaattc gcctcatcca atgcaacaac
    11521 gcggaggaag catgagtaag aaaattgccg ttttaatcac tgatgaattt gaggattcag
    11581 aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt accattgaaa
    11641 aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat
    11701 ccatcgatga agtgacgcca gcggagtttg atgccctgtt gctaccgggc ggtcattcac
    11761 cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt gtgaatagcg
    11821 gcaaacctgt tttcgccatc tgtcacggtc cgcagttgct gatcagcgcc gatgtgattc
    11881 gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat gcgggcgcgg
    11941 aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc cggacaccgg
    12001 acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga gtcgcgtagc
    12061 caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc atcgagccgg
    12121 atttcccata ccggggccga cagcattctg gcaaccggaa agcgacgatt gtacgccttg
    12181 cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa ctgcacaccg
    12241 cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaactgc cgcctgcggc
    12301 ccgctcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc
    12361 gcatcaaaaa gataaaaggc attagcgcac cagagttccc cttcctgctg cacacaccag
    12421 gtgacaacat gttgttttgc cagccaacgg ctgatggcga tgagtgtttc cattgctgtt
    12481 ctcttttata ctgtgggcct gaccttaaca tgcgatgcag acgatgacac cctggtttct
    12541 ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg
    12601 ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact
    12661 gacgctggcg ttttctgctc cagtgggcga tcgttcgctg gcgttacggg cggaatatcg
    12721 cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg cagggtttgc
    12781 agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac
    12841 tcaaccaggc cggtaacgcc attcagcgcg tcatccgcta aacgatgtac ctggaaggcg
    12901 ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc aaagccgaaa
    12961 cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc
    13021 gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca
    13081 accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta
    13141 aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga
    13201 aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga
    13261 cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt
    13321 cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg
    13381 ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca
    13441 tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc cgacgctgga
    13501 agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg
    13561 cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg
    13621 aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa
    13681 actccagctc gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa
    13741 cttggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca
    13801 aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc
    13861 atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa
    13921 attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt
    13981 taactaaaat cccagttcgt taacattttt gcgttttgat agcgcaacct tcaggaaaaa
    14041 ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg
    14101 gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac ttcgccggcc
    14161 ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca
    14221 aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt tggcagcgcg
    14281 ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc
    14341 tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg caggcttcgg
    14401 cgaccaatga agaggcgatt aacttttatc atcgccattt tgacgttgct cgcgtggtgc
    14461 tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggttact cctgtcccgc
    14521 tggaagtgtt tgctttcggc agcctgtgca ttatgtcgga aggtcgttgc tatctgtcgt
    14581 cgtatctgac gggtgagtcg cccaacaccg tgggagcgtg ttctccggcc cgtttcgtgc
    14641 gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt
    14701 atcaggacgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat ctggtggacg
    14761 gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg
    14821 agttaatggc tgcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt
    14881 atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc
    14941 aaaactttgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc
    15001 aaaccactct tggcgcatat caccgtaaat ggcagtgaga aaagcaatga aatattcctt
    15061 agggccagtg ctgtggtact ggccaaaaga gacgctggaa gaattttatc agcaggccgc
    15121 caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc gtcgggcaac
    15181 caaagttggc gactggctgg agatggcaaa atcgctcgcc gggagtggta agcagattgt
    15241 gctctccacg ctggcgctgg tgcaggcatc atctgaactg ggcgaactga aacgctatgt
    15301 tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga
    15361 acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacactgaa
    15421 aatattgctc aaacagggca tgatgcgctg gtgtatgcca gtggagcttt cccgcgactg
    15481 gctggtgaat ctgcttaatc agtgcgatga gctgggcatt cgtaaccagt ttgaagtgga
    15541 agtcctgagc tacggtcatc tgccgctggc ctactccgcc cgctgcttta ccgcgcgttc
    15601 ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa
    15661 cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg
    15721 ctacgtttac aacctcggta acgagctggc atccatgcag ggcctggttg atgtggtccg
    15781 cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa
    15841 tggcgctgca ccactgccgt tgacggcgaa cagcgactgt aacggctact ggcggcggct
    15901 ggccgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag ttgcatgtgc
    15961 tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg attcactctc
    16021 ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta acaactttaa
    16081 ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt atgactgata
    16141 aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct tcagcgcgag
    16201 aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc tatcatcgct
    16261 actggctggc ggaacaccac aatatgaccg gcattgccag tgctgcaaca tcggtattga
    16321 ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc gtgatgttgc
    16381 ctaaccactc accgttggtc attgccgaac agttcggcac gctcaatacg ctctatccgg
    16441 ggcgaatcga tttggggctg ggtcgtgctc cgggtagcga tcagcgaacc atgatggcgc
    16501 tacgtcgtca tatgagcggc gatattgata atttcccccg cgatgtcgcg gagctggtgg
    16561 actggtttga cgcccgcgat cccaatccga atgtgcgccc ggtaccaggc tatggcgaaa
    16621 aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg gcgcagcttg
    16681 gtctgccgtt tgcgtttgcc tcacacttcg cgccggatat gttgttccag gcgctgcatc
    16741 tttatcgcag caacttcaaa ccgtcggctc gactggaaaa accatacgcg atggtgtgca
    16801 tcaatattat cgccgccgat agcaaccgcg acgctgagtt tctgtttacc tcaatgcagc
    16861 aagcctttgt gaagttgcgc cgtggcgaaa ccgggcaact gccgccaccg attcaaaata
    16921 tggatcagtt ctggtcgccg tccgaacagt atggtgtgca gcaggcgctg agtatgtcgt
    16981 tggtaggtga taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg
    17041 ccgatgagat tatggtcaac gggcagattt tcgaccacca ggcgcggctg cattcgtttg
    17101 agctggcgat ggatgttaag gaagagttgt tgggatagtg tgtcttaacg cgggaagcct
    17161 tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat caggcattgt
    17221 gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg cagtaaatta
    17281 aagctcgata aaatatggac cagtgcgttg ccaacgccaa acaccagaat cagcgtaatc
    17341 atcggcttgc cgccccagac gcggaatttc gggctgccaa agcgtttacg cgatgcgcgg
    17401 gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc ataaccaatg
    17461 gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc aaaggtcagc
    17521 aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc tgccagatag
    17581 tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt tgagaacacg
    17641 accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg taccagcaca
    17701 tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat gttacccatc
    17761 gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta caccaggcat
    17821 ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggcttggcac gttaccgtga
    17881 taaccaaacg acgccaggca gaacggcaat gtcatcagca gatacggcgc ataagatgca
    17941 ttgctttctg caacgttgaa caatgtcgca ggctgcacat gccccaacag gctgccaaag
    18001 gtgaggaaga aggtaatgac tttcgccccc agcacaatcg ccgtcatgcg gctgacggcc
    18061 ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc cgccgcccgt
    18121 gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaatcgaacc actggcagaa
    18181 atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt gaccacgttc
    18241 cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat tcggtaattc
    18301 aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt aaagatcagc
    18361 gccgccattg accagaaaaa ccacgccccg gacatgagca ctggcagaga aaacatccct
    18421 gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg tgacgtttgg
    18481 gtggtggtta gtgttgccat gagggcttct ctccagtgaa aaatagtgcg actgcgttgt
    18541 tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc ccgatggtaa
    18601 aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga tgattacgca
    18661 tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg gccttcacga
    18721 cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc accgctgaag
    18781 ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg acgctcaccg
    18841 ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt gagaatgcga
    18901 gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc gatggtggag
    18961 tgagaagcaa acagcttgat gttaccaatg taacggctgc tgatgtcgcc ttcgttagca
    19021 atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc aatgcggtac
    19081 agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg gtcaccacgc
    19141 gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc
    19201 ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc cattttcagc
    19261 agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg ctgaattttg
    19321 ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg tactttagcg
    19381 gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc tacttccgga
    19441 atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg gttctcaacg
    19501 aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg gtgaacgtaa
    19561 gactcagaat ccatcggaat atcgtagtta actaccaggc tgatacgctc aacgtccagg
    19621 ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt caggcgttcc
    19681 agtgtctgtt cacgaagcgc ctggttcatg tcaccgttca gcgcggcgct gttgtagccg
    19741 ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac gaagataatc
    19801 gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt gcgcataccc
    19861 cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc
    19921 acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt
    19981 gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt ttcaacgtct
    20041 tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc gctcagttta
    20101 gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt accgacaacg
    20161 atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc gccgtacaga
    20221 gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc agcaacctgt
    20281 accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag ctcaggatca
    20341 agattctgca gcagaggtaa agagaatgct gcagtttttc cgctccccgt ctgggccata
    20401 cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat tggagatggt
    20461 ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc cagatctgca
    20521 aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg cggccagtct
    20581 acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac cggctcaaag
    20641 taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat caaaaaagat
    20701 tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca tcttggtcct
    20761 ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt
    20821 tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga cttaggtagt
    20881 acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg ttatccgttg
    20941 cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg tagaactcga
    21001 caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc aacacttctt
    21061 tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta cggaaaggat
    21121 cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg tcacgaccgc
    21181 cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa
    21241 gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt aaatatatgc
    21301 ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt
    21361 tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca tataaaagct
    21421 gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata cgtgccagaa
    21481 tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt ttacgccagg
    21541 aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag
    21601 gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc ggttgctaac
    21661 aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg caaccttact
    21721 cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt
    21781 ctttaatgct cagacggata cggccctggc gatcaacttc cagaactttc accggtactt
    21841 cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg atttgagaga
    21901 tgtgtaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag tcaacgatac
    21961 gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc tcttcgatac
    22021 gacgaatagc gtgtttcgct ttctcgccgt cggtcgctgc gatcttcaca gtaccgtcat
    22081 cttcgatttc gatggtggtg ccagtttctt cggtcagggc acggataaca gaaccgcctt
    22141 taccgataac gtctttgatc ttgtccgggt tgatcttgat ggtatggata cgcggtgcga
    22201 actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc aggatatgca
    22261 gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg ataccttcaa
    22321 ttttgatatc catctgcaat gcagagatac cgtcgcggga acccgcaact ttgaagtcca
    22381 tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt
    22441 ctttcaccag acccattgcg atacccgcaa cggcagcttt gatcggcaca cctgcgtcca
    22501 tcagcgccag agacgcgccg cacacggaag ccatagaaga ggaaccgttg gattcagtga
    22561 tttcagacac aacacgtacg gtgtacggga atttgtccat atccggcatg actgccagca
    22621 cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc
    22681 cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggtg tcggtacgtt
    22741 cgcccatcag ttcatcaaga acctgcgcgt cacgagcagt acccagcgtt gcggtaacca
    22801 gcgcctgcgt ttcaccacgg gtgaacagcg cagaaccgtg agtacgcggc agcacgccag
    22861 tacgcacatc cagaccacga atcatatctt tttcacgacc gtcgatacgc ggttcgcctg
    22921 ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca cccagttcgt
    22981 tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat ttgatgacat
    23041 caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc agacgagctt
    23101 cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc tgccagtccc
    23161 aacgtggttt accggcttct ttcaccagtt cattgatgtt ctgaataaca acctgctgtt
    23221 gttcatgacc gaacactact gcgcccagca tctggtcttc gctcagcaat tcagcttcag
    23281 attcaaccat cagtacagcg gcttcagtac cagcaacaac cagatccagt ttgctctctt
    23341 tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct acgcgggcag
    23401 caccaatcgg accattgaac ggaataccag acagagacag tgctgcggaa gcaccaatca
    23461 tcgcgacgat atccggatta acttgcgggt taacagaaac cacggtggcg ataacctgaa
    23521 cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga
    23581 tcagggtttc gccttcgctt gggcggcctt cacgacggaa gaagctaccc gggatacgac
    23641 cagcagcgta agtacgctcc tgatagttaa cggtcagtgg gaagaagtcc tgacctggtt
    23701 tggctttttt ctggccaaca acggtaacga ataccgcggt gtcatccatg ctaaccataa
    23761 cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg tgttggccgt
    23821 actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt cttagacagt
    23881 accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact aatgacaacc
    23941 ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct gcaacggaag
    24001 atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag
    24061 gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga cccaggcgct
    24121 cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc agtttacgac
    24181 gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg tgctctgcaa
    24241 agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact tcggtagaac
    24301 cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct tcagtactta
    24361 gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct aattcagcga
    24421 tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg gcagtattct
    24481 actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc aaccaccagg
    24541 cgacgaggcg caacgcggcc ttcatcgtca atttcgccca taccgataaa tttgccgttc
    24601 tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg aactgggtta
    24661 ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta gtccgaagct
    24721 ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat atcctgctgt
    24781 tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc aaccggatat
    24841 ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag tttttcaccc
    24901 aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc cagctccagc
    24961 tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg cgcttcacgc
    25021 ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata tttcagtgct
    25081 gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc cgccgccagt
    25141 tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc atcagaagta
    25201 tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc cagcagatac
    25261 tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgccagttgc cagcgggtcc
    25321 agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt ttgcagcgca
    25381 tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg
    25441 cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt
    25501 cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca accagagagt
    25561 tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc cccagcaggc
    25621 tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc gcgtcttcat
    25681 ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga gacatttcga
    25741 caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc tgcaggatga
    25801 gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca
    25861 taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta atttcaggtg
    25921 gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc aacggctgcc
    25981 ggatgcggcg taaaagcctt atccggccta caacccagac aaccttaagc gatggtacgt
    26041 tggatttcga tgatctcgaa tacttcgatc acatcgccag tgcggacatc gttgtagttc
    26101 ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc tttgaagcgg
    26161 cgcagggact ccagctcgcc ttcgtagata accacgttgt cgcgcagaac gcggatcggg
    26221 ttgtgacgtt taaccacacc ttcggtaacc atacaacctg caatggcacc aaatttcggt
    26281 gatttgaata cgtcacgaac ttccgccaga ccgataatct gttgtttcag ttccggagac
    26341 agcataccgc tcattgccgc tttcacttcg tcgatcaggt tatagatgac ggagtagtaa
    26401 cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc acgtacatta
    26461 aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc ggtgatacca
    26521 cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt cagcaaggag
    26581 tcggagatcg cttccacaga accctgtacg tctgccttca ggacaatgtt cacttcgtga
    26641 acttcacctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg ctgacgcgcc
    26701 agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc tttcttctcg
    26761 tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc gaggatttcc
    26821 accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg catcgcacga
    26881 acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt accttcacgt
    26941 accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat aaccgcaccg
    27001 ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag caggatagcg
    27061 tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa ctggctttca
    27121 ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac acgatccgga
    27181 tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg cgccgctttc
    27241 gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc aacaaccagg
    27301 actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc ggcgtgcccc
    27361 ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc accaatgtgc
    27421 tgggtaatgc cgcccgcttc gccagaggcc actttcgttg aacgaatgta gtccagcaga
    27481 gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc
    27541 gcagcccccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc acgacgcagg
    27601 ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc gataacctgg
    27661 ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga gcctttaacc
    27721 gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac gtcacggtta
    27781 acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg ttttccgcct
    27841 ttaccgccac gtactgctgc gcgagcttct tcacgatcag cttttgattc agcgtgtttg
    27901 ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacggcc gccttcgact
    27961 tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac gtgataatcg
    28021 ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc catacgacgt
    28081 gcttcttcag caacgcgacg tgcttcttct tcgagtttac gacgcgcttc ttcttcagct
    28141 ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg ggcgttttta
    28201 gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg tttcgcttgt
    28261 tcagcagctt cacgcttagc ttgttctgcg gcctcacgtt cagctttttg ttgcgcctcg
    28321 cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc
    28381 tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg
    28441 cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg
    28501 cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca
    28561 atcaaagtct gtttctcttg tgcggacaca gagtcgtcag cagacttccg gatacctgca
    28621 tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta
    28681 atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac
    28741 cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg
    28801 atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg
    28861 gcggccagtt tgaatgccaa atcacgatct atcccttcaa ggttcagcag atcgtcagcc
    28921 ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag tgcattttta
    28981 gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct
    29041 ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac cagaacagtc
    29101 gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc
    29161 gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca accgctcagc
    29221 tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccggct
    29281 tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc
    29341 ggtgccattg cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata
    29401 cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacacag
    29461 gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc
    29521 ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg cacttcaata
    29581 cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc
    29641 gcttccgggc gaacggaata gagcacgcca cgaacgcggt cgccagggcg gaagttttca
    29701 cgcggcagca tatcttcgcg caggatcacg gcttcagcat tgttgcccag atccagagag
    29761 atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg
    29821 aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt
    29881 gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg
    29941 cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc
    30001 tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga
    30061 tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc tgtcgccagc
    30121 gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct
    30181 tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa
    30241 aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct
    30301 tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga
    30361 cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag
    30421 tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca
    30481 acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa
    30541 tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg
    30601 cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc agtaatcatc
    30661 tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggccta
    30721 aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa
    30781 ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc
    30841 cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt
    30901 gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg
    30961 ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaag atgcaaatac
    31021 tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg
    31081 ccacattttg tcaacgttta ttgctaataa cgtgaatgaa tatccagttc actttcattt
    31141 gttgaatact tttgccttct cctgctctcc cttaagcgca ttattttaca aaaaacacac
    31201 taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa
    31261 aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta
    31321 tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttccggcg
    31381 gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat
    31441 atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca
    31501 tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag
    31561 gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca
    31621 acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag
    31681 atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct
    31741 atcgttatgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg
    31801 actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt
    31861 tcgactacaa aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa
    31921 cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccgatta
    31981 tgggcgtgaa attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct
    32041 ttgagcaagg tcatccggta gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga
    32101 tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaaa
    32161 accgtatcat cgaagcgaaa agccgtggta tttacgaggc tccggggatg gctctgctac
    32221 atatcgctta tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc
    32281 atgcacatgg tcgtcagttg ggccgtctgc tgtaccaggg gcgttggttt gactcccagg
    32341 cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta
    32401 ccctggagct gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga
    32461 cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc
    32521 gtattggtca attgaccatg cgtaatctgg atatcactga tacccgagag aaactttttg
    32581 gttatgccaa aactggcctg ctctcctcct ctgccacttc aggcgtgccg caggtggaga
    32641 atatggaaaa caaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc
    32701 ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc
    32761 atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctcaagcg tttgcggctg
    32821 ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtaag ctgtcgaaaa
    32881 gatgttgttt tccgttgtgc gatccacgcc atccccaaga acggggctat accagataaa
    32941 catcggaaca tgatatgcct gctggctagc ctccctgcct ccatgaaaat agacgttctt
    33001 cttcgtaggg tcacgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc
    33061 ttttaataat tcaaaaacct gacctagcaa actatctgtg taatgaatgg agttgtcata
    33121 gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat aggcgctaca
    33181 agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct gcgtattttg
    33241 ctgtaatgct tgcgataaat gcggcaacaa caattcatca aatcctctga cataaactgt
    33301 ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa aagcggattg
    33361 cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa tgttgtcggg
    33421 gtaattatga atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg gaaccgacag
    33481 cgcagtgtaa ggtgcgccgc ttattgcttg attaaacagt ttgatctgtt ttctttgtgc
    33541 ttcaacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt cgacacgtac
    33601 agactccccc acaatcaaca cgtaggtatc aatacctgta tccctgactg ataattgaaa
    33661 atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc tctttcgctg ctaaagcaaa
    33721 atagttgaga ttgaaaaacg gcgtataggt agcaaatcgc gacgctagta tatatggact
    33781 gaacgctttt ttattttttg catctttata agcaaattga caagcggaaa ataaactgcc
    33841 cgaaatgaca atcagcaata atattcctgt cacttttttt gtcggcaagg aaacatcata
    33901 ttttattatt actgccaaaa aaagaaggga taaaaaagca aaggcacata gataagggat
    33961 atacatcccc agcattttga caacttcatc cggatcactc tggagcacac taatcgcaaa
    34021 tccatcattg aaagttgtac caaaggtaca ccatgaatag aggctaatac tcatgtctgc
    34081 cgcagtcagt aacacaaatg gtgcggcaac aagcaggcga agcggaaggg atgatatacg
    34141 ttttacaacc aggatggtca ggacgaagaa cagcaatcgc tgaaccatat gcgcgccgga
    34201 agaggcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca accaatgaga
    34261 tttaaaactt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    34321 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
    34381 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcggccatt gcgttctcag
    34441 taattaaata acattatcaa tgcgtctcta aacattacgc agaacgtagc catcagtagt
    34501 tatactgaca ggagcaggat ggtttatcga gaagtaaaca tatccgtatt cgtaccggaa
    34561 ctggagttga cacaataaag tgccaattat gtcagcagaa gggaaaagtt tataactaag
    34621 gcgtaccggc accatcgttt caaggtacca gctacgagta aagcaactgg acgagataca
    34681 gatacctgac aaccattcct cagaccagga ccaaaacgaa aaaagacgct tttcagcgtc
    34741 tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta ttgggcacta
    34801 ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact actttttagt
    34861 tcgggatatc gctggtcggc ttagccggag cagctggttg agtttgttcg gttttcaccg
    34921 gtgcactcag attttcccat tcgctacctt tattggtttt gttgctattg atgttaccca
    34981 gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg gtcatgaagt
    35041 taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag gaggctccca
    35101 tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccaatt gccacaataa
    35161 ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag ttgccgcgta
    35221 ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc gggtgtgaat
    35281 actaaccaaa gcgaatgacc ttcgcaaggg caattttatc gcattgtatc aactgcggaa
    35341 aaaaacagca aaaccgatgt gttcgctgag aaaaaaggcg acaatcgccg cctttttagc
    35401 cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg caaattcagt
    35461 cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg tgccggattt
    35521 acgcagcaac acgcgtccac ggttgcccag cgcagcttca acctctgcgg tcacggcttt
    35581 aactgactca tgctcaagtg gatcgccgct acctgcggtg taacgtacgt taaccagaat
    35641 ctgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatggt tacgtgacat
    35701 cgccgccagc acctgcaagc cagcaacgat gccgtcaccg gtagtagttt tatccagtag
    35761 gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca ttttttccag
    35821 tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt tcagcgccag
    35881 ttcaagcccc atgttgctca tcaatgtacc cacagcgcca ccacgcagct ggccctgacg
    35941 aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat tgccttcatg
    36001 gtcgaccata atcacgcgat cgccatcgcc gtcgaaggca ataccgagat ccgctttttc
    36061 agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc cccacttcgg cattgatgtt
    36121 tacaccgttt ggctcacaac cgatagcgat aacgttcgcc cccagttcgc gcagcacgtt
    36181 cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca gttcactgag
    36241 gctaagttcg ttcgggaacg tggctttgca aaactcgata tagcgacccg cggcatcaac
    36301 gatacggctg gctttaccca gttctgccga atcaacgcag ctgatctcct tttccatttc
    36361 cgcttcgatg gcctcttcta ccgcatccgg cagtttggtg ccgtcgatag agaagaattt
    36421 aatcccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg cctctgcgcg
    36481 gaaggtacgc gtcagataag ccacggccgg tgttggcatc gggccagtga agagtgcgga
    36541 aaggcccgct gccgccagac ccgcttccag tgctgactcc agcatatagc cagaaatacg
    36601 cgtgtcttta ccaataataa tcttacggga gccgtggcgc gccagcactt tacccgcggc
    36661 ccaacccagc ttaagcacaa aatcaggtgt gatcggcgca tcccctacac gaccacgaat
    36721 cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt gcagacagag
    36781 tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca cgaatgatgt
    36841 gcgcgccttg cattgcggca atgaccgcac aggccagact accgctcagg cgctcggacg
    36901 gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc aacagcggca
    36961 ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg gagagatttt
    37021 taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc gcctgctcgc
    37081 aacgtgctat ttgctcaata aagtagcgat tcacttctgc aaagacatcg tcatacttcg
    37141 gagcttcctg catggttttt ggatttccct gcatatgcat cagacaaacc ggtaaaccgg
    37201 tttctgcagc cgcctccaga gcgccaggtt cggaaaggga gcggatatca ttaataatgt
    37261 gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg accgagatcc
    37321 agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc aactcttctt
    37381 caacgctaac ttccgccgcc cctgggcgcg tggactcgcc accaacgtca atgatcgtcg
    37441 cgccagcatt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag ttatgcgtgc
    37501 caccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga ggatggctaa
    37561 ggtccagtga agtaccctgg gcaaagagtt tcatgatgtt atccctggta tgaattgata
    37621 agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat ctgatgcggg
    37681 aacttacttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg tacgcggttc
    37741 atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag aagcgcctgg
    37801 ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat caatctgcgg
    37861 tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa tatccatatt
    37921 gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt tcacttcctg
    37981 gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc tacggccgag
    38041 gaatacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag agaagcccca
    38101 ctgggtcacc atgttacgtg ccagattggt cgcaacttta atatcgttgg acgcaccggt
    38161 agaaacatgt tccggcccat agatgatctc ttctgccaga cgaccaccgt acagcgtaga
    38221 aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag gcaagaagaa
    38281 agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttgtgcaccg gatcgtgttc
    38341 cggcaccagg cgaccgataa tcgcatgacc cgcttcgtgg taagccgttg attctttctg
    38401 cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt ctttcgcttt
    38461 ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca gtgccgcttc
    38521 gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacggg caatgattgc
    38581 cgcgtcgata tcgggggcca atggtacgcg acgcatgtga actttcagga tctgctcacg
    38641 accgcgaaca tctggcaagc cgaccacaac ctgacggtcg aaacggccag gacgcagcag
    38701 ggccgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac cttcgttacc
    38761 ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt catcgtgacc
    38821 accgcccaga ccagcgccac gctggcggcc tacggcgtcg atttcatcga taaagatgat
    38881 gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg cacccacacc
    38941 gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt tcgcttcgcc
    39001 tgcaatcgct ttcgccagca acgttttacc ggtacccgga ggaccgacca tcaggacgcc
    39061 tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat actctaccag
    39121 ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg tcgttttgat
    39181 ctgatcttcc gtcagcatgc gcgctttgct cttaccaaac gacatggcac ctttgccacc
    39241 gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca gcatcgggaa
    39301 ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac cgacaacctt
    39361 gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa tgtaagtggt
    39421 ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac gcgcttcacg
    39481 aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac ggccattaga
    39541 ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga ccagccagag
    39601 tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact gtgttaaaaa
    39661 cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat ctcccggtta
    39721 gggtttacgc ccggtcgcta caatatacac ttcccgcgaa cgtgcacgag aagagtccgg
    39781 cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact catcgaaacc
    39841 ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat cacgacacat
    39901 ttctaacgcc agttccacca gatacatggc acgggggata tccaccgccg gtgttccgct
    39961 catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa cgcgctccag
    40021 cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca caccaacgat
    40081 aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa tttgggtgac
    40141 cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca ttcccggttt
    40201 aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg aacgtaaccc
    40261 ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc agcggctgga
    40321 gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact catcgtaacc
    40381 aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg tagaatgacc
    40441 cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa caaaaacagc
    40501 acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt aatggtttga
    40561 ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc atcaaggtga
    40621 aaatcgccac cgaagatcgc gaaactaaaa ccttgatcgt ggaagctatc gtgcgcgaaa
    40681 ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc ccaactaaag
    40741 aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt tgctgtgtaa
    40801 aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt tgataaaagg
    40861 ccgcatagcg gcctttttcc tttctttaca atacatcaac atcttgagta ttgggtaatt
    40921 cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc gttttgatga
    40981 ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag tttacagaaa
    41041 tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc tgttcttcgt
    41101 cagaatccag attcagcacc gttacggtag caccaaaaat aacgcgccca ttgttgggca
    41161 ttttggtgac atcaatcacc tgcgcgttcg acagcttggc ttcgatgtct ttaatacggc
    41221 cttcgcagaa accctgctgt tcacgagctg cgtggtattc ggcgttttct ttcaggtcgc
    41281 catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca gatttcagaa
    41341 aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata gcttgcattt
    41401 gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc tgttcggcct
    41461 gcccggcata actcctcacc gggaccagaa gcaaaaaaat accgacccgg gtacaagtcc
    41521 caggtcagct acaattcaca ttttgatagt cattttaccc tgaagttccc gaagggtcat
    41581 cgtttacttt atagggcgtt gcgccgtagt atgacggctc gattccaggt tgttagcgcg
    41641 agattatgcg attttccaga tttatcatcg gattgaccag ctgtatagcg ttcagtgttc
    41701 aggccgcgaa tgttgatgag tacgttactc aactccccgc tggtgccaac cttgccctga
    41761 tggtgcaaaa agtcggcgcg tcggcccccg ctattgatta ccacagtcag cagatggcgc
    41821 tgcccgccag tacgcagaaa gtgattactg cgctggcggc gttgattcaa ctcggccccg
    41881 attttcgttt caccacgacg cttgaaacca aaggcaatgt ggaaaacggc gtacttaagg
    41941 gtgacttagt ggcgcgattt ggtgccgatc cgacgttaaa acgtcaggat attcgcaata
    42001 tggtcgcgac tttgaaaaaa tctggcgtca accaaatcga tggcaatgtg ttgatagata
    42061 cctccatttt cgccagccac gataaagccc ccggctggcc atggaatgac atgacacaat
    42121 gctttagcgc tccgcctgcc gctgccatag ttgaccgcaa ctgtttctcc gtctcgctct
    42181 acagtgcccc aaaacctggt gatatggctt ttatacgcgt ggcatcttat taccccgtta
    42241 cgatgttcag ccaggtacgc accctccccc gaggttctgc cgaagcgcaa tactgcgaac
    42301 tggatgtcgt accaggcgac ctgaaccgct ttacgctgac gggatgcctg ccacaacgtt
    42361 ctgagccgct cccgttggct tttgccgtgc aggatggagc cagctatgcc ggggcaattc
    42421 tgaaagatga gttaaaacag gcaggtatca cctggagcgg aacactgctg cgccagactc
    42481 aggttaacga acctggaacg gtagttgcca gtaaacagtc ggccccgttg cacgatctgc
    42541 ttaagattat gctgaaaaag tcggacaaca tgatcgccga tacggttttc cgcatgatag
    42601 gccatgcgcg cttcaatgtg cctggaacat ggcgggccgg gtcggacgcc gtgcgtcaga
    42661 tcctgcgcca gcaagccggt gtcgatattg gaaacaccat tattgccgat ggttcagggc
    42721 tttcgcggca taacctgatt gcccccgcca ccatgatgca ggtgctgcaa tacattgccc
    42781 aacacgacaa tgaacttaac tttatctcca tgctgccgct tgcgggctat gacggctctt
    42841 tgcagtaccg tgcaggtctg catcaggcgg gcgtggatgg aaaagtctca gcgaaaaccg
    42901 gttcgttgca gggggtatat aacctggcgg gattcattac cacagcgagc gggcaacgaa
    42961 tggcgtttgt acaatatctt tctggctatg cagtagaacc ggcggatcag cgtaatcgcc
    43021 gtattccgtt agtgcgtttt gaaagccgtt tgtataaaga tatttatcag aacaattagt
    43081 caaaaagaaa ccccggcaca tggccggggt tttgctgatt aacgcttgta aatgaactca
    43141 acgccttctt cgtcgtcttc gtcccagtca tcatcccagt cctcatcatc ctcttcagca
    43201 atctcttcaa gctgctggcg atgataatca tcccacatga attcgacttt ctctggctgt
    43261 ttcgcttctt cagcctgcac gaccgggttt tcaatgataa aggtcatcac atcccagcag
    43321 agatctttca cgcccagtcc actcgccgca gagatcagat aatatttatc ttcccagccc
    43381 agcgcttcag caatcgcttt cgctttctct tcagcttctg ccttatccag cagatcgatc
    43441 ttattgaaca ctaaccaacg tggtttcgcc gccagatcct ggctgtattt ttccagctcg
    43501 ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt caatcggatc gatatcgatg
    43561 aggtgcaaca ggacgcggca acgttccagg tgcttcagga agcgaatgcc cagacctgcg
    43621 ccttccgcag cgccttcaat cagtcctgga atatcggcaa caacgaagct cttttcgttg
    43681 tccattcgta ccacacccag acttggcacc agagtggtaa acggataatc cgccactttc
    43741 ggtttagccg ccgataccgc acgaataaag gtcgatttac ccgcgtttgg catccccaac
    43801 ataccgacgt cagccagcag catcagctcc agcagcagct cacgcttatc gcccggcgta
    43861 ccgttagttt tctgccgtgg tgtacggtta actgacgatt tgaaacgggt attgcccaga
    43921 ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt gtttggtcat atcgcccatg
    43981 gtttcaccag taccctggtc gattacacgc gtacctaccg gcactttaat tgtgacgtct
    44041 ttaccgcgct taccggtaca gtcgcggctt gcgccattct gaccgcgctc tgcacggaaa
    44101 gatttttcaa aacgataatc gataagcgtg ttcaggttct cgtcggcttc catccatacg
    44161 tcaccaccat caccgccgtc gccgccatcc gggccgcctt tcggaatata cttttcgcgg
    44221 cggaagctca cgcaaccatt accgccatca cctgcaacga ccagaatcga tgcttcatca
    44281 acaaacttca ttttattctc cgtaaatcat tcgcctgcgc ggggttgcga taccaccgtt
    44341 gtatgcttac gtaatccgcc ccaaatacga tgaccaatgg cggaatacat cgcgcccgca
    44401 accacgacaa acgcaccgag ataacctaaa aggtttaaca tcggtctggc gaagaaatcg
    44461 ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa acagtggggt gagcgtgatg
    44521 atcgcgctca cctgcgctgc ctgccagcga gccatcgctt ccgccagggc gccatatcct
    44581 accaaggtat tcagtccgca aaaaattaaa catgcgagct gccagtggct aagctgcgct
    44641 atcactccag gctttgccag agggaagagc gcaattgtac ataaagtgta cagtaaaaac
    44701 aggatctgcg gtgaggccag ccgacgcaat aaaacctttt gcgccacgcc ataactcacc
    44761 caaaccgtcg ccgcaccgac cccaaagata actccccagg tgtaatcggt gagctttgta
    44821 aatatctcga ccagactggt gttaaaaaac atcaccaggc cgctcaggag catcaatgcc
    44881 cctacaacct gagtgctgcg cattttctct ttcaggataa atacgctggc aaccatcatg
    44941 ccaactggcg agagttgccc aatcacctgc gaagcggtcg gactcaggta ttgcaaggat
    45001 gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg ccactgccaa caaaatcaac
    45061 cagcgtggct tacgaaacac gcgtaatggc ggcaacctct tcttcaccgc aagaatggca
    45121 ccaaggccaa tactcgccat caagaaacgg taaaacacga ttgtcggagg ttccatcacc
    45181 tccagcacct gcttcattgc gattggcaac gccccccagc aaattgctgt ggtgagcgcc
    45241 aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt ccctgcagag cgcatttgcg
    45301 gattaacgtc gcaccatgcg catttttacc ggttatcgaa tgtaaaaagc cccgcaacgt
    45361 gttgcggggc tttcatccgt taccgggacg cgaaaaactt attcagcttc gatgctgata
    45421 aatttacggt ttttcgggcc tttaacttcg aatttcactt taccgtctgc tttagcaaac
    45481 agagtgtggt cacgaccgca acctacgtta gcgccagcgt ggaatttggt accacgttga
    45541 cgaacgatga tgctacccgc cagaacggat tcgccaccga aacgcttaac gcccaggcgt
    45601 ttagcttctg aatcgcgacc gttacgtgtg gagccgccag cctttttatg tgccatttga
    45661 aatctctcct caggtcttag gcgctgatgc cagtaatttt cacatcagtg aaccactgac
    45721 gatggccctg ctgcttacga tagtgtttac gacgacgaaa cttaacgatt ttaactttct
    45781 cgccacgacc gtgagcaaca acttcagctt tgattacgcc gccatcaacg aaaggaacgc
    45841 cgattttgac ttcttcaccg tttgcgatca tcagcacttc agcgaactca acagtttcgc
    45901 cagttgcgat gtccagcttt tccaggcgaa cggtctgacc ttcgcttact cggtgttgtt
    45961 taccaccact ttggaaaacc gcgtacatat aaaactccgc ttccgcgcac accttttcaa
    46021 tgattcagag tgcgctataa atattcacaa tagggcgcga atattacgca aaacgcacgc
    46081 ctttgacaag tgctacagtc aatacacgaa gaaaaaaaac acaacttgta cggtaacgtt
    46141 tatctgtgcc attttttcag tacaatcact ctatattcct aaccataaac cctaagttgc
    46201 ctttgttcac agtaaggtaa tcggggcgaa aagcccggct tttgcgatga atttagaaaa
    46261 aatcaatgag ttaaccgcgc aagatatggc gggtgttaat gcggcaatcc ttgagcagct
    46321 taattccgac gtccagctga tcaatcagtt aggctattac atcgtcagcg gcggcggtaa
    46381 acgtattcgt ccgatgattg ctgtactggc tgcacgagct gttggctatg agggaaatgc
    46441 gcatgtcacc attgctgccc tgatcgagtt tatccacacg gcgactctgc tgcacgacga
    46501 cgttgtggat gaatcagata tgcgcagggg taaagctacc gccaacgctg catttggcaa
    46561 tgccgccagc gtgctggtag gcgattttat ttatacccgc gcgttccaga tgatgaccag
    46621 cctcggttcg ctcaaagtgc tggaagtcat gtcagaagcc gtaaacgtca tcgcagaagg
    46681 tgaagttctg caactgatga acgttaacga tccggacatc actgaagaaa actacatgcg
    46741 cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc gcgcagtgtt ccgggattct
    46801 ggctggctgt acgccggaag aggagaaagg cctgcaggat tatgggcgct atctcggcac
    46861 tgctttccag ttgatcgacg atttactcga ttacaatgcc gatggcgaac agttaggtaa
    46921 aaatgtcggc gacgatctga acgaaggtaa accgacgctg ccgctgctgc atgcgatgca
    46981 tcatggcaca ccagaacagg cacagatgat ccgtaccgcc atcgaacagg gtaacggtcg
    47041 ccatcttctg gaaccggttc tggaagcaat gaacgcttgt ggatctcttg aatggacgcg
    47101 tcagcgtgcc gaggaagaag cagacaaagc catcgcagca ttacaggtgc tcccggacac
    47161 cccttggcga gaagcactca tcggcctcgc gcacatcgct gttcaacgcg atcgttaatc
    47221 ccctcccttc atcccgcgca gtgcgcggga tgattccagt aaattccata aaaacactaa
    47281 ttcagcgcta taacacctga aaaaaagggc gatgaatctt tatatattct gaatattcac
    47341 actctttaca ggaacttttt agagcaatag gccatcagga gtatagtgat gcccgacaga
    47401 agaagtgttc tgaatgaaag cgactaactt aaggagtgag gaaagatgga aagtaatttc
    47461 attgactggc atcccgctga catcattgcg ggtttgcgca agaagggaac gtcaatggcg
    47521 gcggagtctc gcagaaatgg tttgagttcc tcaacgctgg caaacgcatt atcgcgccca
    47581 tggccgaaag gagagatgat tattgcgaaa gccctgggaa ctgacccctg ggttatctgg
    47641 ccatcacgct accatgatcc gcagacccat gagtttatcg acagaacgca gttgatgcgt
    47701 agctacacta aaccgaaaaa atgagtggtc tggcggtagc cccgcgaacg gggctgccag
    47761 ctctcagacg attactcgcc tttcacacgc tcaatatttg cacctaaagc gcgcagtttg
    47821 tcttcaatgc gttcgtagcc acgatcgatg tgataaatac gatcaaccac cgtcgtccct
    47881 tccgcaatac agccagccag caccaggctt gctgatgcac gcagatcggt tgccataacc
    47941 tgtgcgccag aaagtttttc aacaccgtga caaataacgg tattgctttc gatttcggca
    48001 tgcgcgccca tacggctaag ctccggcaca tgcataaagc ggttttcaaa gaccgtttcg
    48061 gtgatgaatc cggtcccttc tgccaccagg ttcaacagcg tgaactgggc ctgcatatcg
    48121 gtcgggaatg ccggatgcgg cgcggtacgt acgttaacag ccttcggacg tttgccatgc
    48181 atatccaggc taatccagtc ttcgccgact tcgatgtccg ctccagcgtc acgcagtttc
    48241 gccagcacgg cgtcgagagt atctggctgc gcgttacggc agataatttt gccgcgagaa
    48301 atcgccgccg ccaccaggaa agtaccggtt tcgatacgat ccggtagcac gcgatagaca
    48361 ccgccgccta aacgttccac accttcgatg acgatacgat cggtgccctg accgctaatt
    48421 ttcgcaccca gcgtaatcag gaagttcgcg gtatcgacga tttccggttc acgcgctgcg
    48481 ttttcaataa tcgtggtacc ttccgccagg gttgcagcac acatgatggt caccgttgcg
    48541 ccaacgctga ctttatccat cacgatatgt gcgcctttca gacgaccatc gacggaagct
    48601 ttaacgtaac cttcttccag tttgatggtc gcgcctaatt gttcgaggcc agaaatgtgt
    48661 agatcaaccg gacgcgcacc gatcgtacaa ccgccaggca gtgaaacttg cccctgacca
    48721 aagcgcgcta ccagcggccc cagtgcccag atagaagcac gcatggtttt aaccagatcg
    48781 taaggtgcgc agaatacatt aacgtcgcgg gcatcaatat gcacagaacc attacgttct
    48841 actttcgcac ccagctggct tagcagcttc attgatgtat cgacgtcttt cagtttcggg
    48901 acgttctgga tctctaccgg ttcttccgcc agtagagcgg caaaaaggat aggcagagca
    48961 gcatttttag cgccggaaat tgtgacttcg ccctggagct tcgttggccc ctgaacacga
    49021 aatttatcca tttagtttgt tctcagttaa caattcatat ccgctaccgg cgaatcgccc
    49081 atagctcaaa agccgttcag tttgcgatcg cgcgcccact ccgcaggggt atacgctttg
    49141 atcgacacag catgaatgcg gttatccgca atatattcca tcagcgggcc atagaccgtc
    49201 tgctgttttt taacccgact catgccgtca aacaactcac ccacggcaat aacctgaaag
    49261 tggctgccat cgccggaaac gtggacttcc tggagggaga gagcgttcat caacacgctc
    49321 tgaatttcat tattttccat gggatcttca atcatcagtt aataaaccag cgaaacatct
    49381 tagagcaaag ttgcgctggc ataaataagc aaaaagcctc gctgataaat cagacaaggc
    49441 tcgacttgca ggcaggtttg ccggacaggc ggttaacgcc atatccggcc tgaaaaaatt
    49501 taacgaggca gaacatcagc aggcaaatta tacaatttcg cgagggtata cactttgtcg
    49561 tttaccccct gaagcgtcac gttgttgccc tgctttttcg ccagatcgat aagatggagc
    49621 agcagtgcca gtccacccgt atccacgcgg gacacacggc taagatcgat gcaggtaatc
    49681 cccttcaccg cttcctcacg catttcccaa agcggtagca gaacgtcctg atccagctct
    49741 ccggataacg ccagcgtgtc acccgtctgc atccagctca gtgactcgct cattattttt
    49801 tctcttccag agtgattttc tgttgagaaa tcgatttcag ttgcgcagtc aggccgtcga
    49861 tacctttggt acgcagcagc gttccccact cgttttgttt ggtggtgatc atactgacgc
    49921 cttcagcaat catgtcgtaa gcctgccagt tgcccgtctg ggagttttta cgccactgga
    49981 agtccagacg caccggcgga cggccattcg ggtcaataat ggtaacgcga ataggcacaa
    50041 tggttttatc gcccagcggc tgttctggcg caatctgata ggtttgaccg tgatacatcg
    50101 ccagcgcctg accgtaagcc tgcttcaggt actcacggaa agcggcaaag taggcttcac
    50161 gttgagcagg ggtcgcactc ttgtaatact ggcccagcac cagcgcaccg gcgtatttca
    50221 cctgtacgta tggcagcagt tcctgatcaa caatggtacg cagataatcc gggttggccc
    50281 gaatttgcgg ttgctcattc ttcaggcgat cgaacgtttt ctgcgccgcc tcgtccatca
    50341 gcttataagg attggtctgg tctgccgcgg ttgccgcact cagaggtgca atcaccagca
    50401 aagcgaccat cattaaacgt ttaaacatgc gtcggttctc ctgaaattat ttcgttgtac
    50461 ccacaggttc agtggtttca ttattacctg gcgcagcagc tggcgcatcg ccactattct
    50521 tattgtcatc gcctttacta ccgtaaagga actgaccaat gagatcttcc agcaccatcg
    50581 cagacttagt gtcctgaatt gtatcgccat ccttcaggat agcagtcccc agttccgggt
    50641 cttcaaaacc gacgtttaat gccagatatt gttcccccag caggccggaa gtacgaatgc
    50701 tcagcgaact ggtatctgga atgtggttat aacgttgttc aatttccagc gttacgcgcg
    50761 gcagataggt tttcgggtcc agcgtaatat ccgccacccg acccacaaca acgccaccaa
    50821 tactgaccgg agagcgcgct ttcaggccgc caatgttatc gaacgtcgca taaagcgtgt
    50881 aggtcggttc agtacgtatg gacgtcacgt tcgccgcctt caggcaaaca aacagcgccg
    50941 ccagcagtgc tgctaataaa aagataccca cccaaatttc attttttttc gtttgcatga
    51001 actcaattcc caaacatcaa tgcggtcagc acaaaatcca gccccagaac agccagagac
    51061 gagtggacaa cggtgcgagt ggttgcccgg ctaatcccgg cagacgtcgg gatggcgtcg
    51121 tagccgttaa acaacgaaat ccacgtcacc gtgatggcga acaccacgct cttaatcaga
    51181 cagttgacca gatccatacg ccagtcgacg gcattttgca ttgccgacca gaagaacccg
    51241 ctatcaatgc ctttccaact gacgccgacc agagatccgc cccagatccc cacggcgacg
    51301 aaaataaccg tcaacagtgg taatgaaata accccagccc agaaacgggg agaaataacc
    51361 cgacgcagcg gatccaccgc catcatctcc atactggaga gttgctctgt agcgcgcatc
    51421 aggccgattt ctgcggttag cgccgaacca gcacgcccgg caaacaacaa cgcggcaacc
    51481 accggcccca gttcacgcag tagcgataac gccaccagca tacccagact ggtttccgca
    51541 ctataagtgg tcagaaccag ataaccttgc agccccaaca ccattccgat gaacacgcca
    51601 gaaaccacaa taatcagcat cgacaggacg ccgacattat agagctggcg caccagcagc
    51661 ggcgcatgtt tgcgaaattc cggtttgccg accagcgcat tgaataacat taacccggcc
    51721 cgcccgaacg ttctcagggt tttaatccct ttatgtccga gcgacgccag cgcatttaac
    51781 agcatgagtg gcttaactcc ctggtaaaag atcagcgtga taatcgccgg caggatagcg
    51841 gaacggaaca ggcccgtcag ctatcccgtc cagaaactga cgtacgcgcg gatcaggatt
    51901 cgcctgcaac gcctgggcac tgccatgagc gacaattttt ttgtccgcca ggatccaggc
    51961 gtgatccgca atacttaaca cttccggcac atcgtgagaa accaccacac aagtcacgcc
    52021 cagcgcgctg ttcagctcag aaatcagctt caccagtacg cccatggtga tgggatcttg
    52081 cccaacaaaa ggttcatcaa acatgatgag atccggctcc agcgcaatcg cacgcgccag
    52141 cgctgcacgc cgcgccatcc caccagaaag ttcagaaggc attagcttag ccgctccacg
    52201 cagccccacg gcctccagct tcatcatcac tgtactatgc aacaatggcg cgggaagttg
    52261 ggtatgttcg cgcagtggat aggcgacgtt gtcaaatacg ttcatatcag tgaacaacgc
    52321 cccggactga aataacatgc tcatccgttt gcgcactgta tacaggcgcg aacgagacat
    52381 cgccggaata ttctcaccat cgaaaaggat ctcaccatga tctggtgcga tttgcccgcc
    52441 aatcagacgg agtagcgtcg ttttaccgat gcccgatggc cccatgattg ccgtgatctt
    52501 ccctcgcggc acggtcaggg aaatattatc gaagatgcag cgattgccac gcgtaaaact
    52561 gacatcgcgc atatcgacta aattcgccac agactgctcc ataattcacc cttcgtcttg
    52621 cgttgatttt ctaagcatgg cgctcaattt aaccttgaac ccaacatatt tacagaatat
    52681 tacccgccgt ggttagcgaa agctggcatt tgttttactt tttagccgca taaagtcaaa
    52741 attaagaatc cgttacggct ttctgaaaat cttcagcgga ccggcgagta tacctgaaga
    52801 aaggacgtta gatgctttta gctacggcac tgttaattgt tggtttactt ttggtcgttt
    52861 acagtgccga ccgcctggtt tttgccgcgt ctattctttg ccgaaccttt ggcatcccgc
    52921 cgctgatcat cggcatgacg gtggtcagta ttggtacatc gttaccagaa atcatcgtct
    52981 cgcttgccgc gtctctgcac gaacaacgcg atttagccgt tggtacagcc ctcggctcaa
    53041 acattatcaa tatattgctg atcctcggtc tggctgcgct ggttcgtcct tttaccgtcc
    53101 attctgatgt tctacgccgt gaattaccct taatgttgtt ggtcagcgtg gtggccggtt
    53161 ccgtactcta tgacggacaa cttagtcgca gcgatggtat ctttctcctc tttctggctg
    53221 tgctatggct gctgttcatt gttaaacttg cacgtcaggc tgaacgtcag gggactgaca
    53281 gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc gtcgcgtttt
    53341 tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg gttgataacg
    53401 ccacggtgct ggcgaattac tttgccatca gcgagttgac gatgggtctg acggcaattg
    53461 ctatcggaac cagcctgccg gaactggcaa ccgcaatagc ggggggtcgc aaaggtgaaa
    53521 acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc atcgtgttgg
    53581 gtttacccgc gctgataacg ccaggagaga ttgatccatt ggcgtacagt cgtgactaca
    53641 gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc tccccgcaac
    53701 cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg gcgatgttgt
    53761 actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca cgtagagtta
    53821 caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga acgtgaatgc
    53881 ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga aaagatgttc
    53941 tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat cgggcgcaaa
    54001 atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc tggtgaagcc
    54061 gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat ctctaactca
    54121 ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca cgtaccgtta
    54181 atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt gcatctgtgt
    54241 gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag caccaccgcc
    54301 acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg ctttactgct
    54361 gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct gctgcgcgta
    54421 aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc cagtctgcgt
    54481 gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg cgatgacaat
    54541 atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga tatgggcgtg
    54601 gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg tgtgcgccct
    54661 ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac ctccgtgatg
    54721 gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact gcgtgcaggc
    54781 gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg cttgcgacct
    54841 gttacggccc tgtcagcgcc gacgttatag caaaagcaga gaacattcgt ctgctgatcc
    54901 tcgatgtcga tggcgtactg tcagatggcc tgatttatat gggcaataat ggcgaagagc
    54961 tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc tctgatattg
    55021 aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt gccacattgg
    55081 ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc gatctgctgg
    55141 aaaaactggc gattgccccg gaaaatgtgg cttatgtcgg cgatgatctc atcgactggc
    55201 cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca ctgttgatcc
    55261 cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc gaagtttgcg
    55321 acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg atatgagtaa
    55381 agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga tcggcattaa
    55441 tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc ccacctataa
    55501 aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct atcgattgat
    55561 tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc agccggtact
    55621 taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata aagccaagct
    55681 gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac ttgtgccaga
    55741 ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga cgcaggatgt
    55801 tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg gtctgaaaat
    55861 gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa catcctatga
    55921 aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt ctggccgcca
    55981 gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt gaatcggacc
    56041 agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc atcgtcaccc
    56101 agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc ggcgaacaag
    56161 gtaaagaagt gattgacggc tacggtaaac cggcaacgtt ctaccagatg caggacaacg
    56221 gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa gattttgtcg
    56281 ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc gataagatca
    56341 cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag cgcgtaacca
    56401 ccgttctggt gccgtcgcag ctgcaggata aaaacaacaa aggccagacc ccggcacaga
    56461 agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca aaagcctata
    56521 aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa attgtcggtc
    56581 tgctggggcc aaacggtgcc ggtaagacca ccactttcta tatggttgta ggcattgtgc
    56641 cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg cctctgcatg
    56701 cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc cgtcgcctca
    56761 gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct gctgaacaac
    56821 gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg cgtgacagca
    56881 tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc gcactggctg
    56941 cgaatccgaa atttattctg ctcgacgaac cgtttgccgg tgttgacccg atctcggtta
    57001 tcgacattaa acgcatcatt gagcacctgc gcgacagcgg cctgggcgtg ctgatcactg
    57061 accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc agtcaggggc
    57121 atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt aagcgtgtat
    57181 accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt agcaggagag
    57241 tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac tggcgatgac
    57301 gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac ttcagcagga
    57361 gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc atgaagaaat
    57421 cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc tcgaacaaaa
    57481 agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca ccgctggtac
    57541 accatccggc accagcggtg actacattga cgatgagctg ccggtctatc agggcgaaac
    57601 gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt tttccgacac
    57661 tgaccgcgct attgctacct ctatcgtcga tgccgttgat gacaccggtt atctgactgt
    57721 cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg acgaggttga
    57781 agccgtcctt aagcggatcc aacggtttga tccggtcggt gtagcggcaa aagatctgcg
    57841 tgactgcctg ctaatccaac tctcccaatt cgataaaacc acgccgtggc tggaagaggc
    57901 cagactgatc attagcgatc atctcgatct gttagccaat cacgacttcc gcactttaat
    57961 gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga tccagtcgct
    58021 cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca ttccagatgt
    58081 gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca gcattccgcg
    58141 tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg atgatgacag
    58201 ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc tggaaagccg
    58261 taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag ccttctttga
    58321 gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg ctgtcgaaat
    58381 gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc cacgaggcat
    58441 ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg gcgaagcttc
    58501 ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc cagcgaaacc
    58561 gttgagcgac agcaagttaa cctctttact gtcggaacaa ggtatcatgg tggcacgccg
    58621 cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc gtaaacagct
    58681 cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga aataacgtcg
    58741 agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt gagcaatatt
    58801 ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc cacacctcag
    58861 atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt caggacatgt
    58921 acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa cataaagata
    58981 aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct gttgtgcggc
    59041 acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga gtgggcaggt
    59101 tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt gttcttaaca
    59161 gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg gaaatcatca
    59221 gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa gctatcctga
    59281 cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg catggcaaac
    59341 tggaagaaga tactctgcgc gccgttggcg ttttcgttca gctcgaaacg cctatagctt
    59401 tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg ccggcagacc
    59461 aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg gacaaaacca
    59521 tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc attacggata
    59581 ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga cgttgtgagg
    59641 agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt aaatctgtcg
    59701 ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc gtagtgttgt
    59761 tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc agcattgatg
    59821 ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac ctgcctgacg
    59881 ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt cgtcgttaca
    59941 gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa agtgctatcg
    60001 acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt gtcgatacct
    60061 cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg ggtaaacgtg
    60121 aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc cctatcgatg
    60181 cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg aaactgcgtc
    60241 caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca gaagtacaca
    60301 attttatcta ccagacgcga agctatcttg aactatggtt acctatgctg gaaaccaaca
    60361 accgtagcta cttgacggtc gccattggtt gtaccggcgg gaagcaccgt tcggtgtata
    60421 ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag tcacgccatc
    60481 gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca caaacaagct
    60541 gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt ttgacgctga
    60601 agtgctgtta cgtaatgacg aaggcaccga ggccgaagcc aacagcgtta ttgcgctgct
    60661 gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggcc cacaggaaga
    60721 ggaagcactg gccgccgtta tcgccctctt taactctggt tttgatgaag attaatcttc
    60781 atcactttga catacaactc ccttcaaact ccccccgaca ataagaaaat cacgtactga
    60841 aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc agaagaacct
    60901 taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc atactggaga
    60961 tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa gtcccctggg
    61021 caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct gtatcaagat
    61081 tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa agtactacgc
    61141 gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg gtaccgttga
    61201 gacggattgc ggaaccggtt atgtctacga tgtgattgca gattttgacg gtaaaccctc
    61261 cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg cacaactgcg
    61321 ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga cgatgtcgct
    61381 gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc cggtcgtctg
    61441 tgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt ggtgttgtct
    61501 gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg ctattgccct
    61561 gcaaaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc gcgaagctta
    61621 atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct gccgtaaaat
    61681 ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac gaagtggatt
    61741 aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg cgggtttgtg
    61801 gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt cggcgatatt
    61861 caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca gcgttaatcc
    61921 agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat ttttggccgt
    61981 ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt tgtgcgccag
    62041 ggctttctca atggaagcga catcaaagcc ccagtgctca ggaaatttct gatcttctgc
    62101 ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc agtcagaatg
    62161 tgccacgtaa cgaaaattgc catgcagcca ggcgctgacc tgtcgctcga ccattaccgc
    62221 tgagaaggga acaggcgcaa cactaaacaa cgcgatgccc ccgccccaga aaacggcgag
    62281 gacaaccatt aaccgcaaaa gaaaacggcg aacgaaacta aacaccgtta agcggctttt
    62341 actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct cgccgcttct
    62401 gcaatgttct gcgccagcat atatgctggg gtggtgacaa tcttattgtc ttcatcaacc
    62461 acgatatcat cgacaggaca gggcacatgc tccgcgccca tctcttccag cacttctgcg
    62521 gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt cggcagcatc
    62581 gccggggcaa tacacataaa accaagcggt tttccggcct gatgcatcgc ttgtgccagc
    62641 gcctttaatt cacggtcaac ggtgcattcg ctaccaagag tggcaaaatt gcttaaattc
    62701 ttcgccgcgc caaacccccc cggcacaatc aacgcatcca gttcagcggc atcggcctgg
    62761 gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac gttgcgcgtt
    62821 tccgtcatcg cttcgccagt taaatggttg ataacatcaa cctgctgctt atccggtgca
    62881 aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac cgcttcatga
    62941 atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt cttcattgtg
    63001 atcatccttt cgcaattgac tgaaacacat taattttttt aataaaaatg gtacgcatca
    63061 cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac tgcgtgcggc
    63121 ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttacggcgc agccaagatt
    63181 tccctggtgt tggcgcagta ttcgcgcccc ccggtctagc cggggtcatt ttttagtggc
    63241 ttttgccacc cacgctttca gcacttctac gtcgtgacgc cactcttctt tcatctcttc
    63301 aatccattca ccgacgttat cttcccaggc aggaaggtcc ggagactgaa tttgctgacc
    63361 cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt tatgtccttc
    63421 ctctacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc tgacatagcc
    63481 cggcatcatc ttctcaaaca ccgctaaccc gtcggtgatc agcttcggtc ctacaagttc
    63541 gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt tctcttctgt
    63601 cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca tcgcggttag
    63661 cgccggaacc gaaagcggct tactcagcac atcatccatt ccagcattga ggtactcttg
    63721 tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct cgcgcggata
    63781 acgcttcgtc agttcacgag agatatccag cccggtcata tctggcaact gaatatccag
    63841 caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc cggtcatggc
    63901 gacatcaacg ctgttaccta atttttccag cacagaacgc gcaacaatca cgttcagttc
    63961 aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt catcaaacgc
    64021 atcatcgacc tcttctgcta ccgacggtgc gtggatcgtc aacgtaaagg ttgaaccttt
    64081 gccctgttcg ctggtaaccg taatatcgcc gcccatattt ttcgccagac gacgagaaac
    64141 ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt tcacctggta
    64201 atacatggcg aaaattttat ccagctcatc ctgcggaatg ccgataccag agtcttccac
    64261 ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct ggccttgctg
    64321 ggtgaatttg acggcgttac tgatgaggtt ccacaggatc tgccgtaaac gcgtcccgtc
    64381 ggtaatgacc tgatgcggta atggcagcgt cggctccagg ttaaagcgca atcctttttg
    64441 ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga aatcaaccgg
    64501 ttgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa tatcgttaaa
    64561 gatattcccc agcgtgacgg ccgaaacatg gatggtcttg agatattttt cctgctcggc
    64621 ggtgagttcg gtatccagca gaatacggct caggccgacg ataccgttca gcggtgtacg
    64681 caattcgtga ctgatggtgg aaataaacgt cgttttgtcg cggctggccc gttcaagcgc
    64741 atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac cgtgacgttt
    64801 acccacgcgg tcgtagtacg gcactttacg gatttcaaag caggctttgc gcccgtccgg
    64861 gtaatccagc cactgttcat aggtcagtga cacattatga cggaacactt tttcatcggt
    64921 ttcaatgact tttgcggcgg cttccggtga gtaaacatca gcaggtttca ggtgaaccag
    64981 ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg aaaactcttt
    65041 atcttcgtta cgataaaaaa ccaggtcggg tgaagcatca aggaaggaac gtaagaatga
    65101 ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa ttttcagttg
    65161 gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcaattt cctgatttag
    65221 ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc gcatctcctc
    65281 cagtttttgc accagccgtg acagacgttg tcgtgactcc tccagttgct cgacgaccac
    65341 cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag aacgaataac
    65401 atcaatgctt tcgacctgac catgcagcac catggttacc gccatttgta ccacaatggc
    65461 aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta acttcatcat
    65521 caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat tccttcacga
    65581 caacctgccc caataatacc caattctgac agttacgtta taaattgtgt gagaaatgcg
    65641 gaattcccct caacgaccta ctccgttgga ggtatccacg gacgttccag cgccgatccc
    65701 tgtacgccat gttcattcag atagcgatcc agctcgacca ttcccgtcca tcgattttcg
    65761 caccacagag gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg gtggtagatc
    65821 acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtgtaatc ctccagttca
    65881 ataccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt cacaatatgc
    65941 agcggatgca gctttatgcc gtccacgccg gtttcaacca ctcgctccag cgtttgcaaa
    66001 cattcagcct gcccttcgcc aggcaggccg acaattaagt gggaacacac cttcagcccg
    66061 cgctgacgtg ccagctgggt tgtacgctga taacaggcaa aatcatggcc gcggttgatg
    66121 cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac ttcgtaaccc
    66181 tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc cgggcgggta
    66241 ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat agaacgcagc
    66301 acctgaactt ccgcaaaggt gctggtatac gcctgaaagt aggccagata gcgtttagcg
    66361 cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg ctgctgcgct
    66421 tcatcggcaa acgaggcaac attacagaat gtgcagccac cacgcccgat ggtaccgtca
    66481 cggttagggc agctaaaacc gccgtgtagc gtcagcttat gcaccttttg cccataacga
    66541 cgggtgagat caccaccaaa catattgact aatttctgta actgcataat ctgatagacc
    66601 gcgccttgaa aagaggccaa agcctgccat ttttagctca attcggcgat gacctggatc
    66661 aatcgtcccg gcctgctttt atcaactgca taatcaatca aaattaccga aatttcatgc
    66721 ataatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc cttatggatt
    66781 ttaggcaaaa acagtagcat gaaacgtcat taccaattaa ggcagtataa aatgctggtt
    66841 ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc aggaattgac
    66901 tgtttctcta acgacttccc ttttagcctt aaagataaaa tccattttaa tttcagtaat
    66961 ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc atttgacctg
    67021 tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag cagcctgctc
    67081 atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg cgaaaaggac
    67141 aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc acgctctttc
    67201 tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg aggttcacga
    67261 tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga tcgcccacat
    67321 agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg cccgcatgca
    67381 gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct tgctgttaca
    67441 aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt tagcaaaaaa
    67501 ctacgccgtc gggatgctat tcctgaataa agatcctgaa ctcgccgctg ccgcacgccg
    67561 catcgttgaa gaagaactgc aacgcgaaac cttgtcgatt gtgggctggc gtgatgtccc
    67621 cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccacgca ttgagcaaat
    67681 ttttgtgaac gccccggcag gctggcgtcc gcgcgatatg gagcgccgtc tgtttatcgc
    67741 ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct gtagcctgtc
    67801 gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc gcttttatct
    67861 ggatcttgcg gacctgcgtc tggaatcggc catctgcctg ttccaccagc gcttctccac
    67921 taacaccgta ccgcgctggc cgctggcgca gccgttccgc tacctggcgc ataacggtga
    67981 aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata aattccagac
    68041 gccgcttatc cctgatctgc acgacgccgc accgttcgtc aatgaaaccg gctctgactc
    68101 cagttcgatg gataacatgc tggaactgct gctggcaggc ggaatggata tcatccgcgc
    68161 catgcgtctg ttagtgccac ccgcctggca gaacaacccg gatatggacc cggaactgcg
    68221 tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggcccgg cgggcatcgt
    68281 gatgtccgac ggtcgttttg ccgcctgtaa cctcgaccgt aacggtctgc gtccggcgcg
    68341 ctacgtcatc accaaagata agctcatcac ctgcgcctct gaagtcggta tctgggatta
    68401 ccagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga tggttatcga
    68461 cacccgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga aaagccgcca
    68521 tccatataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg aagatctgcc
    68581 cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg ctcgccagct atcagaaaca
    68641 gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg aaaacggtca
    68701 ggaagcggtc ggttcgatgg gcgacgacac cccattcgcc gtgctctcca gtcagccgcg
    68761 cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc caatcgaccc
    68821 gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa tgaacgtctt
    68881 ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc tgctctactc
    68941 cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata cgctggatat
    69001 cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctgt gcgacaaagc
    69061 cgaaaaaatg gtacgtagcg gcaccgtgct gctggtgctc tccgaccgga atatcgctaa
    69121 agatcgcctg ccggttccag ccccgatggc ggttggcgcg atccagaccc gtctggtcga
    69181 tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc gcgatccgca
    69241 ccacttcgcc gtgttgctgg gcttcggcgc gacggctatt tatccatacc ttgcctatga
    69301 aacgctgggc cgcctggtag acacccatgc gattgccaaa gattatcgta ccgtgatgct
    69361 caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa tgggcatctc
    69421 caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctgc acgatgatgt
    69481 agtgggcctg tgcttccagg gggcggtcag ccgcattggt ggggcgagct ttgaagactt
    69541 ccagcaggat ctgctgaatc tgtcgaaacg tgcctggctg gcgcgcaagc ccatcagcca
    69601 gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcctacaacc cggacgtggt
    69661 gcgcacgctg caacaggcgg tacagagcgg cgagtatagc gactatcagg aatacgcgaa
    69721 gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta cgccgggtga
    69781 aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac gctttgatac
    69841 cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg cggaagcgat
    69901 gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg cgcgctatgg
    69961 cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg ttactccggc
    70021 gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga agccaggcga
    70081 aggcggtcag ttgccgggtg ataaagtcac tccttacatc gccaaactgc gctattcggt
    70141 gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta tcgaggactt
    70201 agcgcagctc attttcgacc tcaagcaggt taacccgaaa gcgatgatct ccgtgaagct
    70261 ggtttccgaa ccgggcgttg gcaccatcgc caccggtgtg gcgaaagctt atgccgattt
    70321 aatcaccatc gcaggctatg acggtggcac cggcgcaagc ccgctgtcgt cagtgaaata
    70381 cgcaggctgt ccgtgggagc tggggcttgt tgaaacccag caggcgctgg ttgctaacgg
    70441 cttgcgtcac aagatccgtt tgcaggtcga tggcggcctg aaaacgggtg tcgatatcat
    70501 caaggcggcg attctcggcg cagaaagctt cggcttcggc actggcccga tggtggcgct
    70561 cggctgtaaa tatctacgta tttgccatct gaacaactgc gcaacgggtg tagcaactca
    70621 ggatgacaaa ctgcgtaaga accactatca cggcctgccg ttcaaggtta ccaattactt
    70681 tgaatttatt gcccgtgaaa cccgcgagct gatggcacag ctgggcgtaa cgcgtctggt
    70741 ggatctgatt ggtcgcaccg acctgctgaa agaactggac ggtttcaccg ccaaacagca
    70801 aaaactggcg ctgtcgaagc tgctggagac cgccgaaccg catccaggta aggcactcta
    70861 ctgcaccgaa aacaacccgc cgtttgataa cggcctgctg aacgcgcagt tgctgcaaca
    70921 ggcgaaaccg tttgtcgatg agcgccagag caaaaccttc tggttcgata ttcgcaacac
    70981 cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg gcgatcaggg
    71041 gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga gcttcggcgt
    71101 gtggaacgcg ggcggcgtgg aactgtacct gaccggtgat gccaacgact atgtcggtaa
    71161 aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct tccgcagcca
    71221 tgaagcaagc attatcggca acacctgcct gtatggcgcg accggtggtc gtctgtatgc
    71281 cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca ccgtggtaga
    71341 aggcattggc gacaacggct gtgaatatat gacgggcggt atagtctgta ttctgggtaa
    71401 aaccggcgtg aacttcggcg cgggcatgac tggcggcttc gcttacgttc tcgatgaaag
    71461 cggcgatttc cgcaaacgcg ttaacccgga actggtcgag gtcttaagcg ttgacgctct
    71521 ggcgatccat gaagagcatc tgcgtggtct tatcaccgag catgtgcagc ataccggctc
    71581 tcagcgcggt gaagagattc tggcgaactg gtcaaccttc gccactaaat ttgcgctggt
    71641 taaaccgaag tccagtgatg taaaagcact gctgggtcac cgtagtcgta gcgcagctga
    71701 gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt atcaatttat
    71761 cgacctgcag cgcgttgatc cgccaaagaa accgctgaag atccgcaaaa ttgagtttgt
    71821 tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggctgacc gctgcctgtc
    71881 gtgcggcaac ccatactgcg agtggaaatg cccggtacac aactacatcc cgaactggct
    71941 gaagctcgcc aatgaggggc gtatttttga agcggcagaa ctgtcgcacc agactaacac
    72001 cctgccggaa gtttgcggac gtgtctgccc gcaagaccgt ctgtgcgaag gttcctgcac
    72061 cctgaacgat gagttcggcg cagtgaccat cggcaacatt gagcgttata tcaatgataa
    72121 agcgttcgaa atgggctggc gtccggatat gtccggcgtg aaacagaccg gtaaaaaagt
    72181 ggcgattatc ggcgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga cgcgcaacgg
    72241 cgtaaaagcg gtcgtcttcg accgtcaccc ggaaattggc ggcttgctga ccttcggtat
    72301 tccggctttc aagctggaaa aagaggtaat gacgcgccgc cgtgaaatct tcaccggcat
    72361 gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtacagctgg acgatctgct
    72421 gagtgattac gatgccgtgt tccttggcgt cgggacttat cagtcaatgc gcggcgggct
    72481 ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttcctcatcg ccaacaccaa
    72541 acagttaatg ggctttggtg aaaccaacga cgaaccgttc gtcagcatgg aaggtaaacg
    72601 cgtggtagta cttggcggtg gcgacactgc gatggactgc gtgcgcacgt ctgtacgcca
    72661 gggagcgaag cacgtaacct gtgcctatcg tcgtgatgaa gagaacatgc cgggttcccg
    72721 ccgcgaagtg aaaaacgcgc gggaagaagg tgttgagttc aaattcaacg tccagccact
    72781 ggggattgaa gtgaacggta acggcaaagt cagcggcgta aaaatggtac gtaccgaaat
    72841 gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt ccgaacatat
    72901 cgttccggca gatgcggtga tcatggcgtt tggtttccgt ccacacaaca tggaatggct
    72961 ggcaaaacac agcgtcgagc tggattcaca aggccgcatc atcgccccgg aaggcagcga
    73021 caacgccttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg tccgtggttc
    73081 cgatctggtg gtgaccgcta ttgccgaagg tcgtaaggcg gcagacggta ttatgaactg
    73141 gctggaagtt taagctctgt cccagcgccg cctcctcaga aaagagtcgg cgctgaaatt
    73201 ctcctttctt tctggaaagc gcctcgcata atctgaagaa aaccaatgta atacgctcat
    73261 ctggattgtt accggtagat gcccggcgta tagctgattc cattattcaa cgcagcgcca
    73321 tataaaggga cagataatgg aatcgctctc agagagaacc tcaacaggct accagcaaat
    73381 ccacgacggt attattcatc tggtcgatag cgcccggacg gaaacggtac gtagcgttaa
    73441 cgcgctgatg accgcgacat actgggaaat tggccgacga attgtcgaat ttgaacaagg
    73501 tggcgaggcc agggctgcgt atggtgcgca gctaatcaag cgactatcaa aggatttaag
    73561 tctaaggtat aagcgtgggt tctctgcaaa aaacttacgc caaatgaggc ttttttacct
    73621 cttttttcaa catgttgaaa ttcgccagac agtgtctggc gaattaacac cattgggaat
    73681 tccgcagaca ccatctgcag aattcccatc ggcaaagatt tggcagacgc tgtctgccaa
    73741 atctttcccc ctgccctggt ccacctacgt ccgtttactt tccgttaaaa acgctgacgc
    73801 ccgcagcttt tatgaaaaag agacgctccg ctgtggctgg tctgttcgcc agctagaacg
    73861 gcaaattgcg acccaatttt atgagcgaac actactgtca catgacaaat cagccatgct
    73921 gcaacaacac gctcctaccg agacgcatat tcttccgcaa caggcgatac gcgatccctt
    73981 tgtgctcgaa tttctggaat tgaaagatga atattcagaa tctgatttcg aggaggcgct
    74041 gatcaaccac ctgatggatt tcatgctgga acttggggat gattttgcct ttgttggtcg
    74101 gcagcgaagg ttacgcattg atgacaactg gtttcgtgtc gatctgctgt ttttccaccg
    74161 ccgtttacgc tgcctactaa tcgtcgatct aaaagtgggc aaattcagct atagcgatgc
    74221 cggacagatg aatatgtatc tcaactacgc caaagagcac tggacgctac cggatgaaaa
    74281 tccgcccatc ggtctggttc tctgtgcaga gaaaggagcc ggagaagcac attatgctct
    74341 ggcaggtttg cctaacaccg ttctggcaag cgaatataag atgcaactac ctgatgagaa
    74401 acgactcgca gatgaactcg ttcgaacaca ggcggtgcta gaggaaggct atagacgccg
    74461 ttaatttcag cggtttcgaa ttctgcagac agtgtctgca gaatctttaa aacgaactaa
    74521 gttttaagcc attaaatcag ccttaacctt caccacaacc tttttaatct ctccaggctc
    74581 gccgacaacg catcctggtt tatgcggttc acctggcata aacacggcga acattcccgg
    74641 ttttaagatg atggcttgct cgttatcaat ggtgctgcaa agctgataat catcctcatg
    74701 gtggaactct tcacactggc gcgcagtgcc tgccatgcca aacagaatcc gttcctcacc
    74761 gtttaataac agttggatat caatgtattg ctcgtgcaat tccgcttttt tctcgacggg
    74821 cgattgagtg ttaaacgtca tgacattcat aaagatattg tcgccctgta attcgtaacg
    74881 acccggcgct ttttcttgcg gtctggcagc taatgccagc gttaacgcgt cctgtaacgc
    74941 aggatgtaac ccagcagacg gtaatgactg tacttcaccc atcatcataa tttttctccc
    75001 tgggccaaca gcgcagcccc aagtaaacct gcatcgtggc ggtagtgcgc cgccagtaaa
    75061 tcaacatgaa atgccgctgg ctcctgcgcc agatacgttt ccaccagcgc cagataccct
    75121 tctgccagcc caacgctgcc accgaccacc acgcactggc aatcagttgt ggctttaata
    75181 tcagcgatca gccttgcaag cgtacgtgcg gagcggtgaa tcagctgctg cgcctgctcg
    75241 tcgccctgcc cggcgcgtgt gaaaatagtt ctcgcatccg cgcccgccag ttcaccctgc
    75301 gctgccgccg caatgccgcg accagaagca atcgcctcca cgcaacctgt gcgtccacag
    75361 ccacagaccg ggccgtgtgg atcggcaagc gtatgcccga tgtgccccgc cagaccgcca
    75421 gggccggtaa gcagtttgcc gccgctcact acaccgccgc cgacgccagt ggaaacggtg
    75481 ataaagacca tatcggttat atcgccttcc agcgcctgat actctgccca tgctgcggcc
    75541 tgcgcgtcgt taatggcaat ggtcggcaaa ttggtaagtt gttccagcgt tttgactaac
    75601 ggaaagtgta gcaatccacc gagattatgc ggattcagcg ccagcaaact gccgtcgcga
    75661 ataattccgg ttgaagcgat ggcaacccgc tgcgcatgag cttgcaacgg agagactaat
    75721 gcggataagg catcacgcaa ggcttctggt gtctggctgg ctggcgtagg aagttcacga
    75781 cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg caagtttagt accgccgata
    75841 tcaatcgcca gcgtggtcat agcaccgcct ttttcatcgc tgtgttgtac cactgacaaa
    75901 tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc ccacgcgccg tggcgcatcg
    75961 catctgccgc ctgagcaggc gtgttgtaac gcccttcggc aatcacccta catccggcgt
    76021 cgctcaacgt tttcaccagc gccagatccg gctcttctgg cgtttcaggc gtggtatagc
    76081 cagaaagcgt agtgccaata atttcggctc ccagcttttg gcatgccagg ccgtcttccg
    76141 gcgttgagca gtcggtcatc gccagtaaac cgtgatggtg aatacgtgcc agcagcgttt
    76201 caacaggcac cggacgcggg cggtcggtgc cgtcaatggc gataatgtcc gcgcccgcct
    76261 gcgccagcgc atcaacatct tcaatatagg ccgtgatgcg taccggagaa tcctccagat
    76321 cgcgtttcac aattccaata atcggcacgc tcaccaccgc acgcgtggct tgcagatttg
    76381 ccacaccttc aatgcgaatg gcaaccgcgc ccgcctgttc tgccgctaat gccatggcgg
    76441 cgacgatttc gggtttatcg agcgggctgt ccggaaccgg ctggcaggag acaatcaggc
    76501 caccgttagc agcgattttt tgatccagtt gtgcaagtaa cgacatacat cttcccttag
    76561 cgaaaggccc ggtacataga ccgggcaaca ggattaactt ttggttttga ctaaatcgtt
    76621 tttggcgctg ccaaatggca cggcaccgct aaatggtttg ccgtcgatag cgtcatgagt
    76681 acgcaatgct tccgggcgca gccaacgctg aacgcgggaa ggcatatcca gaccaatcag
    76741 caggatcacc acaaatgtca gactgaacga tagcgatgcc agcgcagtac ccagatccag
    76801 acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg ccgcccaatg cgccaacgtt
    76861 gtaggtaaag cccaggcccg ctgcacgctg gtcggtatcg aaataaccgc caatcagttt
    76921 cggtaagatc ccggcgatcc cttgtccaag catttgctgg aagaacagta acagaccgag
    76981 cacccagacg tttgcgccgc caatcgcaaa taccggaata atcaacagct gcgaggccaa
    77041 caggctacaa acgtaggctt tgcgggttcc cagccagtca ccgaggaagc cgccgacaca
    77101 gcaccccacc gcagccccaa agccgctaaa gaacagcaca ttggcgacag tatgcgggtc
    77161 ataagccaga tcggttttca gatacgttgg cagcagcgcc tgaatcggcc atgagtagag
    77221 gaaagcaaac aacacgacca ccatcagcat tacgcccgtt ggccagcgtt tgcccgtact
    77281 ctgcaccata aagctgataa agattgcggc gcataacagc ccaagaacag cgacgatcgc
    77341 ggcattttgc aggttaccgg cgaagcagaa ccacagcgca gtagccgccg ccagtgtcat
    77401 tacgatattg gcaatgcgat gttcaccacg gtagagaata tccaccattg tgcgtaccgg
    77461 tgctttaccc ccgtgtttct ctttccagtc ttccgcttcc gggatgtttt tacgcagcca
    77521 gagagcaaag atgattggca aaatgccgat aaagaacagc gcacgccagc cccagaccgg
    77581 aaccaccagg ctatagacct gagcggcaac gacggccccc acagagaagc ctgaaatcag
    77641 aaaaccactg gctttgttac gcagatgttt tggccagctt tcaatgacat aggtggcgct
    77701 ggaaccgtat tcacccgcca tccccatgcc gatgaccaga cgcgcgataa acatggtgat
    77761 gtagcctggc gcaaagccgc aggccagcgt cccggccgag aagagaacga tgctggtgac
    77821 cattgccaga cgacgcccgt agcggtcacc catagcgccg agcatcaggc cgccgaacca
    77881 gcgagagata aaggctgcgg agatcagact tgccgcctgc accgtcgtca gcccgaattc
    77941 accctgtact tcggtgagta ccagggcgat taaaacgaaa tcaaaaccgt caagcagata
    78001 tcccaaccag gcagcggaaa atgcgcgcca ttgtgcacgg ttgagatggc gataccacgg
    78061 gatattctgg gttgtagtac tcattgtgag tctcccgcgg tgggcgatgc ccacacgctt
    78121 tggtataaaa attgtagggt acagatgcgt ttatttcccc tcacccggta ggggcgagcg
    78181 aggggaaaca actcacccgc gctcttgcat caactgctgg gccagcgcct tcagttctgg
    78241 cagatatttt tcatctaccg gtccaaacgg tttgcggcac agcggcacag aaacgacatc
    78301 catataatgg aggacagttt tcaggccgcg gaatacgccc gttttgatca gtaaatcaat
    78361 gactttattg cattcagttt gcagtttctg cgcggtctgg atatcgcctt ctttcagcgc
    78421 cttaacgatc ccctgatagc gccagcccat gatgttgtag gtactaccga taccaccatc
    78481 agcgcccgcc agcagaccag aggcgaagat ttcgtcgtaa ccgttataga gcacaagatc
    78541 aggatgttca cgacggatct gctccatctg atagagatcg ccagaggtct gtttcagcgc
    78601 acctacgcca ggcaatgtaa caagtgtgtt gatctgatcc agggtcagtt ttaccccact
    78661 cagggctgga atgttgtaca ccaccatcgg caaaccatcc gctgaatcaa taattgcccg
    78721 atagtgatcg cagtgttctt caaagctgaa aggatagtag aacggcgtga cggcggagac
    78781 ggcatcgaag ccataacgtt tagccgatgc cgcaagttgt tggctttcgg cggtgctgac
    78841 gcaaccgacg tgggcgatga gtttaatctt acctttcgcc tcttcggcga cgatttccag
    78901 tacctgttca cgctcggaaa ggctttgtac aaaggcctcg ccggtcgaac cacccacgta
    78961 taaaccgtcg atgccctgct gaatattgaa ctgaaccagg cgacgcagac tcgctttatc
    79021 cagtgcttgt tgttgatcaa aaggagtcag gagtgcagcc attacgccac gtaaattcgt
    79081 tgccataaat acctctgaag tgatgcttgt ctgataaacg atataccttt atacctgtta
    79141 taccagatca attaagcaac accccataca gaaagcttat aatgcgatct gcttcactaa
    79201 agtggcatta tttctttttg ttggtggtct gaccgaaagc gtgccaggta gcagagacgc
    79261 tgttgagatg cgattgcaac gcacgatcgg cttcgtcagg atcatgacgg cggatcgcat
    79321 caacgatcgc aatatgctgt tgataactaa cgttgttatg ttcgtgcagt gcctgatcgg
    79381 taaccgttgg gcgtgcggca ataagccagt cgagcagggc aacgtggatc gccatgaaga
    79441 ttgggttacc ggggatctcc gccagcacgc ggtggaaatc aacgtctgaa cgaatgaatg
    79501 ccgcgttgtt atccagcgac tgactgttaa tttccagtgc ttttgccagc aaatcgattt
    79561 gctcatcggt ggcatgttca gccgcatagc gcaccaggct ggattcaaag aacagacgta
    79621 attgttcgaa atgggcaatc ccaccaggat gagaaaggaa atctttcgcc atgccggaaa
    79681 gctcgccgat gatggtgtcc gcagaaggac gtgagacgcg ggcacgttcg ccgttgttga
    79741 tttgcaccag acctttgcgt tttaacgctg ccagcgcttc gcgcacagaa ggacgcccga
    79801 cgttaaagaa cgccatcagt tcgcgttcag acggtaattg ttcaccttcg ccaaattcac
    79861 gacggcggat catctgttcc agctcttctt ccaccatttc ggagagtttt ttacgcgcca
    79921 gcgggcggct acgcaagttg cgaccaattg caggtgaaga atcttcggtt tgcgaatcaa
    79981 atgcgttcat aaggcccatt ctgtaaggtc agtgtgatta acatcatcag tgacatccta
    80041 tcacaggatt gaaagtaggg gaaaatggca ggtttttctc tttgtgcctc atcattacca
    80101 taattaacgg aataattaa
//
LOCUS       DAZGXZ010000006        73058 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000006 DAZGXZ010000000
VERSION     DAZGXZ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73058
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.006"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(208..1191)
                     /gene="zntB"
                     /locus_tag="ACHKJF_000495"
     CDS             complement(208..1191)
                     /gene="zntB"
                     /locus_tag="ACHKJF_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP0947916.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(1205..1252)
                     /gene="ydaN"
                     /locus_tag="ACHKJF_000496"
     CDS             complement(1205..1252)
                     /gene="ydaN"
                     /locus_tag="ACHKJF_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP0947917.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            1446..2678
                     /gene="dgcM"
                     /locus_tag="ACHKJF_000497"
     CDS             1446..2678
                     /gene="dgcM"
                     /locus_tag="ACHKJF_000497"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP0947918.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKLLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(2699..3262)
                     /gene="smrA"
                     /locus_tag="ACHKJF_000498"
     CDS             complement(2699..3262)
                     /gene="smrA"
                     /locus_tag="ACHKJF_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP0947919.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(3592..4500)
                     /gene="abgR"
                     /locus_tag="ACHKJF_000499"
     CDS             complement(3592..4500)
                     /gene="abgR"
                     /locus_tag="ACHKJF_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0947920.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            4676..5986
                     /gene="abgA"
                     /locus_tag="ACHKJF_000500"
     CDS             4676..5986
                     /gene="abgA"
                     /locus_tag="ACHKJF_000500"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP0947921.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            5986..7431
                     /gene="abgB"
                     /locus_tag="ACHKJF_000501"
     CDS             5986..7431
                     /gene="abgB"
                     /locus_tag="ACHKJF_000501"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP0947922.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            7465..8991
                     /gene="abgT"
                     /locus_tag="ACHKJF_000502"
     CDS             7465..8991
                     /gene="abgT"
                     /locus_tag="ACHKJF_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP0947923.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            9002..9517
                     /gene="ogt"
                     /locus_tag="ACHKJF_000503"
     CDS             9002..9517
                     /gene="ogt"
                     /locus_tag="ACHKJF_000503"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP0947924.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            9712..10464
                     /gene="fnr"
                     /locus_tag="ACHKJF_000504"
     CDS             9712..10464
                     /gene="fnr"
                     /locus_tag="ACHKJF_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP0947925.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            10616..11566
                     /gene="uspE"
                     /locus_tag="ACHKJF_000505"
     CDS             10616..11566
                     /gene="uspE"
                     /locus_tag="ACHKJF_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP0947926.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(11616..11873)
                     /gene="ynaJ"
                     /locus_tag="ACHKJF_000506"
     CDS             complement(11616..11873)
                     /gene="ynaJ"
                     /locus_tag="ACHKJF_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP0947927.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            12117..13148
                     /gene="ynaI"
                     /locus_tag="ACHKJF_000507"
     CDS             12117..13148
                     /gene="ynaI"
                     /locus_tag="ACHKJF_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP0947928.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(13199..14812)
                     /gene="mppA"
                     /locus_tag="ACHKJF_000508"
     CDS             complement(13199..14812)
                     /gene="mppA"
                     /locus_tag="ACHKJF_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP0947929.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(15149..16048)
                     /gene="pgrR"
                     /locus_tag="ACHKJF_000509"
     CDS             complement(15149..16048)
                     /gene="pgrR"
                     /locus_tag="ACHKJF_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP0947930.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            16186..17106
                     /locus_tag="ACHKJF_000510"
     CDS             16186..17106
                     /locus_tag="ACHKJF_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0947931.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            17106..18038
                     /locus_tag="ACHKJF_000511"
     CDS             17106..18038
                     /locus_tag="ACHKJF_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0947932.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            18056..18694
                     /locus_tag="ACHKJF_000512"
     CDS             18056..18694
                     /locus_tag="ACHKJF_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0947933.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            19003..19731
                     /gene="mpaA"
                     /locus_tag="ACHKJF_000513"
     CDS             19003..19731
                     /gene="mpaA"
                     /locus_tag="ACHKJF_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP0947934.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19706..20671)
                     /gene="ycjG"
                     /locus_tag="ACHKJF_000514"
     CDS             complement(19706..20671)
                     /gene="ycjG"
                     /locus_tag="ACHKJF_000514"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP0947935.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            20790..21296
                     /gene="tpx"
                     /locus_tag="ACHKJF_000515"
     CDS             20790..21296
                     /gene="tpx"
                     /locus_tag="ACHKJF_000515"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP0947936.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(21340..22881)
                     /gene="tyrR"
                     /locus_tag="ACHKJF_000516"
     CDS             complement(21340..22881)
                     /gene="tyrR"
                     /locus_tag="ACHKJF_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP0947937.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(23029..24090)
                     /gene="ycjF"
                     /locus_tag="ACHKJF_000517"
     CDS             complement(23029..24090)
                     /gene="ycjF"
                     /locus_tag="ACHKJF_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP0947938.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(24087..25484)
                     /gene="ycjX"
                     /locus_tag="ACHKJF_000518"
     CDS             complement(24087..25484)
                     /gene="ycjX"
                     /locus_tag="ACHKJF_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP0947939.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            25639..26637
                     /gene="ycjW"
                     /locus_tag="ACHKJF_000519"
     CDS             25639..26637
                     /gene="ycjW"
                     /locus_tag="ACHKJF_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0947940.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEESFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(26748..27653)
                     /gene="ompG"
                     /locus_tag="ACHKJF_000520"
     CDS             complement(26748..27653)
                     /gene="ompG"
                     /locus_tag="ACHKJF_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP0947941.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(27698..28780)
                     /locus_tag="ACHKJF_000521"
     CDS             complement(27698..28780)
                     /locus_tag="ACHKJF_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0947942.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(28794..29453)
                     /gene="pgmB"
                     /locus_tag="ACHKJF_000522"
     CDS             complement(28794..29453)
                     /gene="pgmB"
                     /locus_tag="ACHKJF_000522"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP0947943.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFLAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(29450..31717)
                     /gene="ycjT"
                     /locus_tag="ACHKJF_000523"
     CDS             complement(29450..31717)
                     /gene="ycjT"
                     /locus_tag="ACHKJF_000523"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP0947944.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSHEAWQRELDFASGELR
                     RNVVWRTSNGAGYTIASRRFVSADQLPLIALEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQKCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     LFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEGE"
     gene            complement(31714..32769)
                     /gene="ycjS"
                     /locus_tag="ACHKJF_000524"
     CDS             complement(31714..32769)
                     /gene="ycjS"
                     /locus_tag="ACHKJF_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0947945.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNGHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(32779..33566)
                     /gene="ycjR"
                     /locus_tag="ACHKJF_000525"
                     /pseudo
     CDS             complement(32779..33566)
                     /gene="ycjR"
                     /locus_tag="ACHKJF_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
     gene            complement(33585..34637)
                     /gene="ycjQ"
                     /locus_tag="ACHKJF_000526"
     CDS             complement(33585..34637)
                     /gene="ycjQ"
                     /locus_tag="ACHKJF_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP0947946.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGHADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(34668..35510)
                     /gene="ycjP"
                     /locus_tag="ACHKJF_000527"
     CDS             complement(34668..35510)
                     /gene="ycjP"
                     /locus_tag="ACHKJF_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0947947.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(35497..36378)
                     /gene="ycjO"
                     /locus_tag="ACHKJF_000528"
     CDS             complement(35497..36378)
                     /gene="ycjO"
                     /locus_tag="ACHKJF_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0947948.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(36399..37691)
                     /gene="ycjN"
                     /locus_tag="ACHKJF_000529"
     CDS             complement(36399..37691)
                     /gene="ycjN"
                     /locus_tag="ACHKJF_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0947949.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPSAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(37705..39384)
                     /gene="ycjM"
                     /locus_tag="ACHKJF_000530"
     CDS             complement(37705..39384)
                     /gene="ycjM"
                     /locus_tag="ACHKJF_000530"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFP0947950.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     NESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(39596..39910)
                     /gene="pspE"
                     /locus_tag="ACHKJF_000531"
     CDS             complement(39596..39910)
                     /gene="pspE"
                     /locus_tag="ACHKJF_000531"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP0947951.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(39985..40206)
                     /gene="pspD"
                     /locus_tag="ACHKJF_000532"
     CDS             complement(39985..40206)
                     /gene="pspD"
                     /locus_tag="ACHKJF_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP0947952.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(40215..40574)
                     /gene="pspC"
                     /locus_tag="ACHKJF_000533"
     CDS             complement(40215..40574)
                     /gene="pspC"
                     /locus_tag="ACHKJF_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP0947953.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(40574..40798)
                     /gene="pspB"
                     /locus_tag="ACHKJF_000534"
     CDS             complement(40574..40798)
                     /gene="pspB"
                     /locus_tag="ACHKJF_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP0947954.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(40852..41520)
                     /gene="pspA"
                     /locus_tag="ACHKJF_000535"
     CDS             complement(40852..41520)
                     /gene="pspA"
                     /locus_tag="ACHKJF_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP0947955.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            41687..42664
                     /gene="pspF"
                     /locus_tag="ACHKJF_000536"
     CDS             41687..42664
                     /gene="pspF"
                     /locus_tag="ACHKJF_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP0947956.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(42784..44049)
                     /gene="puuE"
                     /locus_tag="ACHKJF_000537"
     CDS             complement(42784..44049)
                     /gene="puuE"
                     /locus_tag="ACHKJF_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP0947957.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(44087..45367)
                     /gene="puuB"
                     /locus_tag="ACHKJF_000538"
     CDS             complement(44087..45367)
                     /gene="puuB"
                     /locus_tag="ACHKJF_000538"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP0947958.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHNTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(45369..46856)
                     /gene="puuC"
                     /locus_tag="ACHKJF_000539"
     CDS             complement(45369..46856)
                     /gene="puuC"
                     /locus_tag="ACHKJF_000539"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP0947959.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGESNMKRVWLEAGGKSANIV
                     FADCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(47131..47688)
                     /gene="puuR"
                     /locus_tag="ACHKJF_000540"
     CDS             complement(47131..47688)
                     /gene="puuR"
                     /locus_tag="ACHKJF_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP0947960.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(47715..48479)
                     /gene="puuD"
                     /locus_tag="ACHKJF_000541"
     CDS             complement(47715..48479)
                     /gene="puuD"
                     /locus_tag="ACHKJF_000541"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP0947961.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            48691..50109
                     /gene="puuA"
                     /locus_tag="ACHKJF_000542"
                     /pseudo
     CDS             48691..50109
                     /gene="puuA"
                     /locus_tag="ACHKJF_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
     gene            50258..50422
                     /locus_tag="ACHKJF_000543"
     CDS             50258..50422
                     /locus_tag="ACHKJF_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947962.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            50412..51800
                     /gene="puuP"
                     /locus_tag="ACHKJF_000544"
     CDS             50412..51800
                     /gene="puuP"
                     /locus_tag="ACHKJF_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP0947963.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSETL"
     gene            51931..52176
                     /gene="ymjA"
                     /locus_tag="ACHKJF_000545"
     CDS             51931..52176
                     /gene="ymjA"
                     /locus_tag="ACHKJF_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFP0947964.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            52489..54132
                     /gene="sapA"
                     /locus_tag="ACHKJF_000546"
     CDS             52489..54132
                     /gene="sapA"
                     /locus_tag="ACHKJF_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP0947965.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            54129..55094
                     /gene="sapB"
                     /locus_tag="ACHKJF_000547"
     CDS             54129..55094
                     /gene="sapB"
                     /locus_tag="ACHKJF_000547"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP0947966.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            55081..55971
                     /gene="sapC"
                     /locus_tag="ACHKJF_000548"
     CDS             55081..55971
                     /gene="sapC"
                     /locus_tag="ACHKJF_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP0947967.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            55971..56963
                     /gene="sapD"
                     /locus_tag="ACHKJF_000549"
     CDS             55971..56963
                     /gene="sapD"
                     /locus_tag="ACHKJF_000549"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP0947968.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            56965..57771
                     /gene="sapF"
                     /locus_tag="ACHKJF_000550"
     CDS             56965..57771
                     /gene="sapF"
                     /locus_tag="ACHKJF_000550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP0947969.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            57839..58192
                     /gene="ycjD"
                     /locus_tag="ACHKJF_000551"
     CDS             57839..58192
                     /gene="ycjD"
                     /locus_tag="ACHKJF_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFP0947970.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQSWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            58560..59348
                     /gene="fabI"
                     /locus_tag="ACHKJF_000552"
     CDS             58560..59348
                     /gene="fabI"
                     /locus_tag="ACHKJF_000552"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP0947971.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            59493..60620
                     /gene="yciW"
                     /locus_tag="ACHKJF_000553"
     CDS             59493..60620
                     /gene="yciW"
                     /locus_tag="ACHKJF_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP0947972.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISALVRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            60688..62622
                     /gene="rnb"
                     /locus_tag="ACHKJF_000554"
     CDS             60688..62622
                     /gene="rnb"
                     /locus_tag="ACHKJF_000554"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP0947973.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(62700..62804)
                     /locus_tag="ACHKJF_000555"
     CDS             complement(62700..62804)
                     /locus_tag="ACHKJF_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0947974.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            62858..64843
                     /gene="pdeR"
                     /locus_tag="ACHKJF_000556"
     CDS             62858..64843
                     /gene="pdeR"
                     /locus_tag="ACHKJF_000556"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0947975.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTPLKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(64825..64953)
                     /gene="ymiD"
                     /locus_tag="ACHKJF_000557"
     CDS             complement(64825..64953)
                     /gene="ymiD"
                     /locus_tag="ACHKJF_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP0947976.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            64990..65163
                     /gene="yciZ"
                     /locus_tag="ACHKJF_000558"
     CDS             64990..65163
                     /gene="yciZ"
                     /locus_tag="ACHKJF_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP0947977.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            65253..66002
                     /gene="yciT"
                     /locus_tag="ACHKJF_000559"
     CDS             65253..66002
                     /gene="yciT"
                     /locus_tag="ACHKJF_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP0947978.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            66271..66489
                     /gene="osmB"
                     /locus_tag="ACHKJF_000560"
     CDS             66271..66489
                     /gene="osmB"
                     /locus_tag="ACHKJF_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP0947979.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(66615..66941)
                     /gene="yciH"
                     /locus_tag="ACHKJF_000561"
     CDS             complement(66615..66941)
                     /gene="yciH"
                     /locus_tag="ACHKJF_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP0947980.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(66941..67678)
                     /gene="pyrF"
                     /locus_tag="ACHKJF_000562"
     CDS             complement(66941..67678)
                     /gene="pyrF"
                     /locus_tag="ACHKJF_000562"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP0947981.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(67871..69040)
                     /gene="lapB"
                     /locus_tag="ACHKJF_000563"
     CDS             complement(67871..69040)
                     /gene="lapB"
                     /locus_tag="ACHKJF_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP0947982.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(69047..69355)
                     /gene="lapA"
                     /locus_tag="ACHKJF_000564"
     CDS             complement(69047..69355)
                     /gene="lapA"
                     /locus_tag="ACHKJF_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP0947983.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(69504..70268)
                     /gene="pgpB"
                     /locus_tag="ACHKJF_000565"
     CDS             complement(69504..70268)
                     /gene="pgpB"
                     /locus_tag="ACHKJF_000565"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP0947984.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            70438..71028
                     /gene="ribA"
                     /locus_tag="ACHKJF_000566"
     CDS             70438..71028
                     /gene="ribA"
                     /locus_tag="ACHKJF_000566"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP0947985.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(71092..>73058)
                     /gene="acnA"
                     /locus_tag="ACHKJF_000567"
     CDS             complement(71092..>73058)
                     /gene="acnA"
                     /locus_tag="ACHKJF_000567"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP0947986.1"
                     /translation="LGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVV
                     GKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVEL
                     VEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASN
                     ELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAV
                     TLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPD
                     PIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASE
                     PIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSD
                     TYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDS
                     PAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDS
                     DVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHR
                     SNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVV
                     PCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        17750 a        19038 c        18024 g        18246 t
ORIGIN      
        1 gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa
       61 gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg caaggtgaga
      121 tattgccctg aatgggtaga gagtttattg acttcgctca aactttgcgg cgtttttgta
      181 tacaaacagc cggaaaaatt gcttttgtta caaccattta ctacgatgca accataaagc
      241 aacaccacca ataagaacaa ctaacagaat acaaaaaatt gaaaatccga attgccaccc
      301 gccgccaggg atcccaccaa ggttgacgcc aaataaccct gtcagaaagg tactgggtaa
      361 aaagaccatt gccatcaatg acattgtata ggtacgacga gctaaatttt cctgcatcac
      421 ctgagcgatt tcatccgcca tcacgccagt ccgtgctata caggcgtcga tttcgtcaag
      481 accgcgccca aggcgatcgg caatatcctg catccgacgg cgttggtcat cgctcatcca
      541 cggcaaacgt tcactggcaa gacgagcata aacatcacgt tgcggtgcca tatagcgacg
      601 catcacgatt aattgtttgc gcagcagagc caggaatcca cgcggtggaa tttgctgatc
      661 aaggagatta tcttcaaggt cgataatttt atcgtgcagc tgctcgataa attcgctgga
      721 atgatcggtc aacgcatcgc acacatccac cagccaccct ccgcaatcgg tcggacccgt
      781 gccctcttcc agatcgctca ccacatcgtc cagcgccagc actttgcgtt gtcgggtcga
      841 aacaattaac cgcccgtcca tatatacacg catggcgacc agttgatcgg ggcgttcatc
      901 ggtgctgccg tttatacagc gcaatgtaat cagcgtgcct tcaccgagac ggctgactcg
      961 gggccgagtg ctctcgcctg ccagcgcatc acgtacgtta ttgggaagca gcggtgttgt
     1021 cgccagccat tgggcgcttt catggtgtac ataattaagg tggagccagc agggatgcgc
     1081 ttcatcaatc acatctgtat tttccagcgg tttaacgccg cctctaccat ccagcatcca
     1141 ggcaaatact gcatccggga cattaacgtc cgatccctta atcgcttcca cagtgcctcc
     1201 atcatcaacg cattattttg tagtctagcc ttctggccct gttacgcaac atctcatcac
     1261 cccattaccc tgaaatgatt aataaaattc tgtctaaatt gaatacaaaa agcaaaatgc
     1321 ttttccgtat acaaaccgtg tgaagtgtta aatagcgtct atcattatca gaattatctg
     1381 atcatatgac gtggcttttt tgcgatcgga tagcaacaaa aattgataaa aataacggga
     1441 tctcaatgat tacgcacaac ttcaatacct tggacttact caccagtcct gtctggattg
     1501 tttcgccctt tgaggaacag ttaatttatg ccaatagcgc ggcgcgactg ttgatgcaag
     1561 acctcacgtt tagtcagcta cgaaccggac cctattccgt ctcctcacaa aaagaactgc
     1621 cgaaatacct ctccgatctg caaaaccaac acgatattat cgaaatcctc actgttcagc
     1681 gtaaagaaga ggaaacagca ttaagctgtc ggcttgtttt gcgagagctg acagaaacag
     1741 aaccggtgat tattttcgaa ggtatcgaag cgccggcaac gctgggttta aaagccagtc
     1801 gctcggcaaa ttatcagcgc aaaaaacaag gtttttatgc gcgctttttt ctgactaact
     1861 ctgcaccaat gttgttgatt gacccctcac gagatggcca aatcgtcgat gctaacctcg
     1921 ccgcgctcaa tttctatggt tataaccatg aaacgatgtg ccagaaacat acctgggaaa
     1981 ttaatatgct cgggcgtcgc gtcatgccta tcatgcatga aatttcgcat ttacccggcg
     2041 gtcataaacc tttgaatttt gttcataaac tggcggatgg atctactcgt catgtgcaga
     2101 cctatgccgg gccgattgaa atttatggcg acaagctcat gttatgtatt gtgcatgata
     2161 ttactgagca aaaactgctg gaggagcagc tggaacatgc tgctcaccat gacgcgatga
     2221 ccggattact gaatcggcga cagttttatc acattaccga gccaggccaa atgcagcatc
     2281 tcgccatcgc tcaggattac agcttgttgc tcatcgacac cgatcgtttt aaacacatta
     2341 acgatctcta tgggcattct aaaggtgatg aggtgttatg cgccctcgcc cgcacccttg
     2401 aaagttgcgc tcgcaaaggc gatttggtgt ttcgttgggg aggcgaagag tttgtcttat
     2461 tgctaccaag aaccccactg gataccgcgc tttcgctggc tgaaactatc cgtgtaagcg
     2521 tggcaaaagt gagtatttcg ggcttaccac gctttaccgt cagcattggt gtggcgcatc
     2581 acgaaggaaa tgaaagcatc gatgaactgt ttaaacgcgt tgatgatgct ttgtatcggg
     2641 cgaaaaatga tggacgcaac cgcgtgctgg cggcataagc cgcggatgcg tctcgagatc
     2701 aacgactgcg cttagcgtgg cgctcccagt tttcttgctt cgcctgcgcc gttttacgta
     2761 gtgcgacgta acacgccccg ctgccgccat gatgcggtag cgcagtgcaa tatgcctgaa
     2821 catcatcaaa ttcggtcagc cagcgcgcca catagctgcg gacaatattg gcatgcgatt
     2881 tatcatcccg tcctttgcca tgaataatca gcacgttacg caaaccatca gccatggctt
     2941 gttgaatgaa actgaacatc attttgcggc actcttccac cggctggcgc aaaagattca
     3001 ggctcgcctg ttgcggatat ttaccactgc gcagcttatc cagcacccca tgttgcaacc
     3061 cttcccgccg aaactccagc ggctgactta gtgggatgat gtcgagaaat ccggtggtga
     3121 ggaaattatc aagctgcagc gtgtcgatac gctgcggcgc acgttggtta cgcgttggat
     3181 gccagtggac atcggtagca cgtttcagcg gctggacatc ttccatggcg tcaagaaaca
     3241 gcgatttgtc gtcaaggttc atgttacatc ctcccgcaat taagagcgcg atatgataac
     3301 cagaccgggt cggtccaaca acgtattacc caaaattcca gtaataagtt ccaaatattg
     3361 ccgatatttt aagcaaaatg cttatgcatg attattcatt cacgatatta ataatgtaac
     3421 ttatattttc gtgaaatctg tcactgaaga aaattggcaa ctaaaggtta aaaccgttat
     3481 aacacagtca ccggcgcaga ggagacaatg ccggacttaa gacgcggatg cactgctgtg
     3541 tgtactgtag agtctggcgg atgtcgacag actctatttt tttatgcagt tttaactttg
     3601 cagatagccg cattctcgcc ggaattgcgt gattaatgac gcggtcagtg gtgtctggcg
     3661 actatcacgc cgctgaatca aataataggc cgctttcggt aaaatttcgc taaccggcaa
     3721 catcaccagc ccctgtccgt gcaagggatc gcagcccatt tcttcaggca gtatgctgag
     3781 aaaatcgctt tttgccacca gactgataca ggctgagaac gtctcgcaga ctacaccgac
     3841 ctgtggcgtt tgcgcctgat cgtcaagcaa ttcactcaac tgtttgtagt aactgccgtg
     3901 tggcgtcggc attgtccagc tgtaatccag taactgtttg atcgaacggg caccaatggc
     3961 ggggtgtccc gggcggcaaa agatcgcgaa ttgcttttcc agtaatttct caaaagtaaa
     4021 ttcgtggtcg tacggtccct gataataggt attgatggtg aaatccaatt ctccctgacg
     4081 caattcatta atcatcgaca ccagttgccc ttccataatg cgtactttta cctgcggatg
     4141 ctgctgatga aaacgagata tgacagctgg catcagactg cgggaaatac tggcccccat
     4201 gccgatatta atctgccctg ccagttgccc ttgtcgttgg cgaatttcct cttgcgctgc
     4261 gcgcagctct tcaagaataa gactggcgtg ctgataaaaa ctttcaccgg catcagttaa
     4321 cgtcacgcct ttactacggc gaaaaaagag ttgtgccgct aatccttctt ccagctcctg
     4381 aatagattta ctcagtgccg gttgcgacat attcaacatt cggctcgctc cgcgaatgct
     4441 gccctgacga gccacttcaa caaaagcacg aatttgatga atttttacct gaaaagccat
     4501 gacgccaccg ataaccgtta tttatcagac caaagaaact ggcatctact ttaatgcaga
     4561 tgattgtgtc agggtaattt atgaacggtt aaaactgtga aaaatcagtt agtgataagt
     4621 aaaaactatc gctacgtgaa ccgggtcaca ctttttactg atgacgggaa aggttatgga
     4681 gtctttgaat caatttgtta attcgcttgc cccaaaatta tcgcactggc gacgtgattt
     4741 tcatcactat gcagagtctg gctgggtgga attccgcact gccacccttg ttgcggaaga
     4801 attgcagcag ctcggctatt cactggcgct gggccgcgaa gtagttaatg aaagtagccg
     4861 gatgggacta cctgatgaac tcactctaca acgcgaattc gagcgcgctc gtcaacaggg
     4921 tgcgctagca caatggattg cggtttttga aggtggtttc actggtatcg tcgccaccct
     4981 ggataccggt cgccccggtc cggtgatggc tttccgtgtc gatatggacg cgctggatct
     5041 cagtgaagag caggatgtca gccatcgccc ctaccgcgac ggttttgcgt catgtaacgc
     5101 cggaatgatg catgcctgtg gtcatgatgg acataccgcc attgggcttg ggctggcgca
     5161 tacccttaaa caattcgagt ccggactaca tggcgtcatc aaactgattt ttcagcctgc
     5221 agaggaaggt acgcgtggcg cgcgggcgat ggtcgatgca ggtgtcgtag atgatgttga
     5281 ttattttact gccgtgcaca ttggcactgg cgtacctgcg ggcaccgtgg tgtgcggcag
     5341 tgataatttt atggcaacca ccaaatttga cgcgcacttc accggtaccg ccgctcacgc
     5401 aggcgcaaaa ccagaagacg gtcacaatgc cttgttggcg gcagcacaag ccactcttgc
     5461 actgcatgca atcgccccgc acagcgaagg agcttccaga gtaaacgtgg gcgttatgca
     5521 ggcaggaagc ggtcgtaacg ttgttcctgc ctcggcgttg ctgaaagtgg aaacacgcgg
     5581 ggccagcgac gtcattaatc aatatgtttt tgaccgtgca caacaagcga ttcagggcgc
     5641 agcaaccatg tatggtgtcg gcgttgaaac tcgtctgatg ggtgcagcta ccgccagttc
     5701 tccttcgccg caatgggtcg catggttgca aagtcaggcg gctcaggtcg cgggggtcaa
     5761 tcaggccatt gaacgtgttg aagcgcctgc gggttccgaa gatgccacat taatgatggc
     5821 ccgcgtgcag caacatcaag ggcaagcctc ctacgtggtg tttggcacac agctggcggc
     5881 aggtcatcac aacgaaaaat tcgattttga cgagcaggtt ctcgctattg ccgtcgaaac
     5941 gctggcgcgc accgcgctca attttccctg gacgcgaggt atctgatgca ggaaatctat
     6001 cgttttatcg acgatgcgat tgaagccgat cgccaacgtt ataccgatat tgccgatcaa
     6061 atctgggatc atccagaaac acgttttgaa gagttctggt cagcggagca tctggcttcg
     6121 gcgctggaat ctgcaagctt caccgttacc cgcaacgtag gcaatatccc aaatgcattt
     6181 attgcttcgt ttggtcaagg caaaccggtt atcgccctgc tgggagaata tgacgccctg
     6241 gcaggtttaa gtcagcaagc tggttgcgcg caacctacat ccgcgacgcc cggtgaaaat
     6301 ggtcacggtt gcggacacaa tttgctggga accgccgcct ttgccgctgc aatagccgtc
     6361 aagaaatggc tggaacaata tgggcaaggc ggcacagtgc gcttttatgg ttgtcctggc
     6421 gaagaaggcg gctcgggtaa aacgttcatg gtccgcgagg gggtatttga tgatgtggat
     6481 gcggcactca cctggcaccc ggaagccttt gccggcatgt tcaatacccg cacgctggca
     6541 aacattcagg catcatggtg ctttaaaggg atcgcagcac atgccgcgaa ttcccctcat
     6601 ttgggacgca gcgcccttga tgccgtaacg ttgatgacca ctggcaccaa cttcctcaac
     6661 gaacatatta ttgaaaaagc gcgcgtacac tatgccatca caaatagcgg cgggatctcg
     6721 cccaacgtgg tccaggcgca ggcagaagtg ctttatctta tccgtgcccc cgaaatgacc
     6781 gacgtgcagc atatttatga tcgggtcgcc aaaatcgccg aaggtgcggc attgatgacc
     6841 gaaaccacgg ttgaatgccg cttcgacaaa gcctgttcca gttatctccc gaatcgcacc
     6901 ttagaaaatg ccatgtacca tgccctatcc cattttggta ccccggaatg gaactccgaa
     6961 gaactggctt ttgcgaaaca aattcaggct acgctcaccc ccaacgatcg gcaaaacagt
     7021 ctgaataata tcgccgcaac cggtggcgaa aacggcaagg tttttgcact acgtcatcgt
     7081 gaaacggtac tggcgaatga ggtcgctcca tatgccgcca ccgataacgt gcttgcggca
     7141 tcgactgatg tcggcgacgt cagttggaaa ctgcctgttg cccagtgttt cagcccctgt
     7201 tttgccgtcg gtacaccgct acatacgtgg caactggtta gccaggggcg aacatctatt
     7261 gctcataaag gaatgctgct ggcggcgaaa actatggcag caaccacagt caatctcttc
     7321 attgattcag ggctattgca agaatgccaa caagagcatc agcaagttac ggacacgcaa
     7381 ccgtatcact gccctatccc gaaaaacgtg acaccgtcac ctttaaaata acaacaacgc
     7441 aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc ctcccaatcc
     7501 gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttccccatcc ttttttgctc
     7561 tttatctatt tgataatcgt actcatggtg acgacggcaa ttttgtcagc ctttggcgtc
     7621 agtgcgaaaa acccgaccga tggtacgccg gtggtggtga aaaacctgct cagtgtggaa
     7681 ggattacact ggtttttacc caatgtgatt aaaaacttta gcggttttgc tccacttgga
     7741 gcgatcctgg cgctggtttt aggtgccggc ctagcggagc gcgtcggctt actgccagcg
     7801 ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat ggtgctgttt
     7861 attgcttttt tcagccacat ttcttccgat gcggcgttag tgatcatgcc accgatgggt
     7921 gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc tgccattgca
     7981 ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt gttgctgtcg
     8041 gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag tgtaattgat
     8101 aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg cttgataacc
     8161 gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga gaaactgcag
     8221 acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc gctacttttt
     8281 attgctgcga tcgcgctgat ggtgatcccg gaaaacggga tattgcgcga tccgattaat
     8341 cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactaatcat tctttttttc
     8401 tttgtggttt cgctggctta tggcatcgct acccgcacaa ttcgacgtca ggcggattta
     8461 ccgcatttaa tgattgaacc gatgaaagag atggcgggat ttatcgtgat ggtttttccc
     8521 ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg ggaaattcat cgccgtgggg
     8581 ctgaccgata tacttgaaag ttcagggctt agcggcatcc cggcgtttgt cggtctggcg
     8641 ttgctttcct ctttcttatg catgtttatt gccagcggtt ccgcaatctg gtcgattctg
     8701 gcccccattt tcgtaccaat gtttatgcta cttggctttc acccggcatt tgcgcaaatc
     8761 ctctttcgta ttgccgactc atccgtattg cctttagcgc cggtatctcc ttttgttcca
     8821 ctgtttcttg gattcctgca acgctacaaa ccagacgcga aactgggtac ttactattcg
     8881 ttagtcttgc cctatccgct tatctttttg gtggtatggc tgctgatgtt gctggcgtgg
     8941 tatcttgttg gtctgccgat aggtccgggg atttacccac gtttgtctta agagagaacg
     9001 gatgctgaga ttacttgaag aaaaaattgc cacgccactg ggtccactgt gggtgatttg
     9061 cgatgagcaa tttcgcctgc gggcggttga atgggaagag tacagcgaac gcatggtgca
     9121 gctgctggac atccattatc gcaaagaagg ctatgagcgc atttctgcca ccaacccagg
     9181 cggtttaagc gacaagcttc gtgattattt tgccggtaat cttagcatta ttgatacgct
     9241 tcccactgcc acagggggga cgccatttca gcgcgaagtc tggaaaactc tacgcactat
     9301 cccctgcggg caggtaatgc attacggcca actggctgaa caattgggcc gccctggcgc
     9361 ggcgcgtgcc gttggtgcgg caaacggatc gaatcccatc agcatcgtcg taccttgcca
     9421 tcgggttatt ggccgaaacg gcaccatgac cggatatgca ggcggagttc agcgaaaaga
     9481 gtggttattg cgccacgaag gttatctttt gctgtaaaca ttaaacaatt tgtgccagct
     9541 tgttcacact tttatgtaaa gttaccctta acaacttaag ggttttcaaa tagatagaca
     9601 tatatttaca tctaatatcg gaattctctg ctgttaaggt ttgcttagac ttacttgctc
     9661 cctaaaaaga tgttaaaatt gacaaatatc aattacggct tgagcagacc tatgatcccg
     9721 gaaaagcgaa ttatacggcg cattcagtct ggcggttgtg ctatccattg ccaggattgc
     9781 agcatcagcc agctttgcat cccgttcaca ctcaacgaac atgagcttga tcagcttgat
     9841 aatatcattg agcggaagaa gcctattcag aaaggccaga cgctgtttaa ggctggtgat
     9901 gaacttaaat cgctttatgc catccgctcc ggtacgatta aaagttatac catcactgag
     9961 caaggcgacg agcaaatcac tggtttccat ttagcaggcg acctggtggg atttgacgcc
    10021 atcggcagcg gccatcaccc gagcttcgcg caggcgctgg aaacctcgat ggtatgtgaa
    10081 atcccgttcg aaacgctgga cgatttgtct ggtaaaatgc cgaatctgcg tcagcagatg
    10141 atgcgtctga tgagcggtga aatcaaaggc gatcaggaca tgatcctgct gttgtcgaag
    10201 aaaaatgccg aggaacgtct ggctgcattc atctacaacc tgtcccgtcg ttttgcccaa
    10261 cgcggcttct cccctcgtga attccgcctg acgatgactc gtggcgatat cggtaactat
    10321 ctgggcctga cggtagaaac catcagccgt ctgctgggtc gcttccagaa aagcggtatg
    10381 ctggcagtca aaggtaaata catcaccatc gaaaataacg atgcgctggc ccagcttgct
    10441 ggtcatacgc gtaacgttgc ctgatttttc cgcataactc actatccttc tgtcatatca
    10501 ttaaattttt ctgatttatt gatctggcag aaggttcatc actgtttcat tcaccagata
    10561 tgggttaatc ttttaattac aaactgcgtt gacagttgtt gtaaggagac cctgtatggc
    10621 tatgtatcag aacatgctcg ttgttatcga tcctaaccag gacgaccaac cagcattgcg
    10681 gcgagctgtt tatttacatc aacggattgg tggcaaaatt aaagcctttt tgccgatcta
    10741 tgacttctca tacgaaatga ccaccctgct ctccccggac gaacgtaccg ctatgcgtca
    10801 gggcgtcatc agccagcgta cagcctggat ccacgagcag gcaaaatatt atctcaatgc
    10861 tggcgttccc attgaaatta aagtggtctg gcataaccgt cccttcgaag ccatcattca
    10921 ggaagtgatc agcggcgggc acgatttggt gctaaaaatg gcgcaccaac atgaccgtct
    10981 ggaagcggtg atttttacgc caacggactg gcatctgtta cgcaaatgcc caagcccggt
    11041 gtggatggtg aaagaccagc cgtggccgga aggaggcaag gcgctggtgg cggtgaatct
    11101 cgccagtgaa gagccgtatc ataatgcgct caatgaaaaa ctggtcaaag agacgatcga
    11161 actggcagaa caagtcaacc ataccgaagt tcatctggtt ggcgcttatc cggtaacgcc
    11221 aattaatatc gcgattgaac tgccggaatt tgatccgagc gtttataacg atgccattcg
    11281 tgggcaacat ttgctggcaa tgaaagctct gcggcagaaa ttcggcatta atgaaaacat
    11341 gacgcacgta gaaaaaggtc tgccagaaga ggtgattcct gatttggcgg agcacttaca
    11401 ggcggggatt gtggttctgg gcacggtagg acgcaccggt atttcagcag cattcctcgg
    11461 caacacggcg gaacaggtga ttgatcatct tcgctgcgat ctgttggtca ttaaacctga
    11521 ccagtatcag acacccgttg aactggatga cgaagaagac gattaacgac tcttcaataa
    11581 ctgacaaggc ccgagttatc gggcctattt tttacttact gctcttcgcc gccaaggaaa
    11641 taaattccca acgggattgc cagcaacaca gtaaagacca ggctataaac aaaaatcatc
    11701 gatgactgaa tcacatacat tgatgtcgtc cactcagaca gcggaatatt gtactgttct
    11761 ataacgccgc cgatagtcgc gcgagtcaca accgacgccg caataatgaa aaccgccaac
    11821 aaaccaaaga gaaatttctt ccctttcgct gacttcagtt tcgccataat catgatgtaa
    11881 tccttgtgca gacaaatttc ttgttaccta aacgtaacaa taccatgaca tttcaccgtt
    11941 gtctgctctg aaaacctcac ctcttagctg gatttacaat caacgcattg attagttagc
    12001 taatgttgct acttccttcc ctgactattg atgatctgaa tgaattgtcg cggtaaaata
    12061 gccccgccag gggtttccgg catgacgaca attatctgga ggagcctgat aaaacaatga
    12121 tcgctgaact gtttacaaat aatgcgctta atctggtcat tattttcggt agctgcgcag
    12181 cattgattct gatgagcttt tggtttcgcc gcggaaatcg taaaagaaaa ggttttttat
    12241 tccatgcggt gcaattttta atctacacca taattatcag tgctgttggt agcattatta
    12301 attatgttat agaaaactat aaactcaaat ttatcacccc aggcgttatc gattttatct
    12361 gtacgtccct gattgcggtt attctgacga ttaagctatt tttgctgatt aatcagtttg
    12421 aaaaacagca gattaaaaaa ggtcgcgata tcaccagtgc gcggattatg tcgcgcatta
    12481 tcaaaatcac cattattgtg gtgcttgttc tgctttatgg cgaacatttc ggcatgagcc
    12541 tttctggctt gctgaccttt ggtggtattg gtggtctggc tgtcggtatg gccggtaaag
    12601 atattctgag taatttcttt tccgggatca tgctctattt cgaccgtcct ttcagtattg
    12661 gcgactggat ccgttcaccg gacagaaata tcgaaggtac agtagcggaa attggctggc
    12721 gaattaccaa aattacgacc tttgataatc gtccattgta cgtaccgaac tcgctgtttt
    12781 cgtcgatcag cgtagaaaac ccaggacgaa tgaccaaccg ccgcattacc acgaccattg
    12841 gtttacgtta tgaggatgcg gcaaaagtgg gcgttattgt cgaagccgta cgtgagatgc
    12901 tgaaaaatca cccggccatc gaccagcgac aaaccttact ggtttatttc aaccagtttg
    12961 ccgactcgtc attgaatatt atggtttatt gctttaccaa aaccacggta tgggctgaat
    13021 ggcttgctgc acagcaagac gtttatttga agattatcga tattgtgcag tcacacggcg
    13081 cggactttgc cttccccagc cagacgctgt atatggataa cattacaccg caggaacagg
    13141 ggcgctaaat cagacatcac tggcgttatc cacaagggta acgccagttc ccatcacatc
    13201 aatgcttcac aatatacata gtccggctgt acgccacatc ttccgggtta ttaatgggat
    13261 aacctttcag ccacggcttg attaatcgtc cattggtata ttgataaatt ggtgcaatcg
    13321 gtgcttgctc catgaggatt ttttctgccg cgttgtaatc ggcattacgc gctttaacgg
    13381 tattttccgt cgatgcctgg gccagaactt tgtcgtatgc cggattgtta aagcgtgaaa
    13441 tatttcctga atgcgttgac gttaataatg tcaggaaagt ggagggttca ttataatccc
    13501 ccacccatga ggcgcggata acatcaaaat tgccggtgtt acggctatcg atataggttt
    13561 tccattcctg attttgcaat ttaacatcta cgccaaggtt ctttttccac atcgatgcta
    13621 cagcaatcgc aattttttga tggttttctg aagtgttata caaaagcgtc agcttcagcg
    13681 gtttttgcgg accataacca gctgcgctca acaaagtttt tgcctgcgca ttcagttctt
    13741 cctgactcat ttgttcaaac ggcgaaggtt ccggcgtaaa tccggcggta acatctggtg
    13801 taaaatgcca cgctggcttt tcgcccgtcc ctaatacttt ttcggtcatc aggcggcgat
    13861 ctatcgtcat acttaatgcc agacgaacgc gctgatctgc cgtcggccct ttttgcgtgt
    13921 taaacgcata ataataggtc ccgagctgcg gcggcgtata aacctgcccc ggaatatcct
    13981 tcaacagctt ctgatacata tttttcggga aggattcggt gatatcaata tcccccgcga
    14041 ggtaacgctt agtggctgcg gattcctgat taattggcag gaaggtcact ttttgcagta
    14101 ccgttttggc gttatcccaa taatgggtat tcggtaccac gaccagtttt tcattgacta
    14161 cgcgatcttt aagaacataa gcgccattgc cgatcagatt tccgggtttc gtccactctt
    14221 taccgctttc tacgttggct ttttgcaccg ggaagaaggc aaagttagcg gttaaattca
    14281 caaaccacgg caacggttta tcaagctgaa ttttcaaggt atgggcatca actgcggtga
    14341 cgccaagctg gtcaggcgta gctttaccat caataatcgc ctgtgcgttg ttgattcccg
    14401 ccagcgcggc aaaccatgca aatggcgata gcgttttcgg gtccaccaga cgctgccagc
    14461 tgtagacaaa atcttgcgcc gttaccggtg tgccatccgc ccattttgcg ttatcgcgca
    14521 gggtaaaagt ccagatacgg ttgtcattac ttttccactg agtcgcaacg ccggggacaa
    14581 tctccccttt ctcgttctga ttcaccagtc cttcaaacag atcgcgaatg acctgaatct
    14641 ctggcaggcc cacggcttta gcgggatcca gcgacgcagg ctcatcttta atgtggcgca
    14701 ccagctcctg cttctctgcc agtactgtgc cgctcggaac ttctgcagca tacgaaagag
    14761 aaatgctgct gaccaacagc gcacaacacg tgactgaaac agagtgcttc ataagtttcc
    14821 ctctaaccga cattaaaatt gatgcgtaat tatttgtttc gccttaaaga aatgcaaaca
    14881 acttccatca aaaaagtgac acaggaagca taaaatgttt tatgtatcaa tatattaaat
    14941 cagatttagt gtaaataatc gacgccaaaa tgcggtaaag cacatagaac accgaacact
    15001 acgttcaggc ttcgcacgtt caaaccagtt ttcggtatca taatcagcat ctgtcgccat
    15061 tgtacgaaga ataaaaaata accaaaacgt tgaaaggaac tgagaatacg ttaagagaga
    15121 aattaaatta atgtatcagc aagtagtgct aaacagcacc tttatacttc agcctatcta
    15181 taaataatga gaatgccgag ccagcatgtc gacggtgtgg atagtacagg tgataaccgg
    15241 gtaaatctgg tgtgaattta tccaaaacac gtatcagttt tttttcttta atagcccgct
    15301 caacctgatc ataaggtaga tacgccaatc catgcccatc aattgcagca tcaatgatca
    15361 ggtctatcgt attcagtaaa agctgacctt ccatgcgaac acgaacttca cgtccaccgc
    15421 gtattaatct ccagcgattt gctgtacccg atgtgggaag atacaaatta attgcctgat
    15481 gatctattaa ttgtgacact gacgttggaa cacttcggcg agaaaaataa tccggtgagc
    15541 caacaatagc cattggtata tctggcccga ttcgaatggc gatcatatct ttatccatct
    15601 ccccacccag acggacgcct gcatcaaaac gttcagaaac gacatcggtc aaaccataat
    15661 caatggtgag ctgaatatca atttcaggat gcgatttcag gaatgtgcgc attgctggta
    15721 acaatatcgt ttttgctgca tgttctacag tagtaatacg tattgtcccg gatgggcggt
    15781 tctgcagatc gctcagggat gccatggctg aatctatgtc atgcaacata gggccaagaa
    15841 cagacaaaag atgctcgccc gcttcagttg gaacaacgct gcgcgtggtt cgcgtcagaa
    15901 gccgcaatcc caatcgttct tctatacgac gcactatctg gcttaaagct gactgcgcca
    15961 tgctcaggcg ggctgctgca cgagtgaagc tacgctcctc tgcaacgacg acaaacgcca
    16021 tcagatcagc gatttcttca cgcttcattc agtatctcca tgcaatctat taatcattta
    16081 aatgataggt ctaatcatga ttttgggtct aatcaactta atgatcaata tctatagtga
    16141 tatcaccaac cgttaaaacg aaccgacaac tccatgagga acggaatgat gaataataaa
    16201 gtcagcttca ctaacagcaa taatccaacc atctctttgt ctgcagtgat ctatttccct
    16261 ccaaaatttg atgaaacccg tcagtatcag gcaattgtgt tatctcaccc aggtgggggc
    16321 gttaaagaac aaacggccgg aacctatgcc aaaaaactgg cggaaaaggg atttgtcact
    16381 attgcttatg acgcatctta tcaaggtgaa agtggcggcg agccgcgtca gttagaaaac
    16441 ccatacattc gtactgaaga catcagtgcc gtgattgatt acctgacaac gctttcttat
    16501 gtcgacaata cccgaattgg tgcgatggga atttgtgccg gtgcaggata tacagccaat
    16561 gctgcaattc aggatcgccg catcaaggcc atcggtaccg ttagtgcggt caatattggt
    16621 tcgatgttcc gtaatggttg ggaaaacaat gtgaaatcta ttgatgccct gccatacgtt
    16681 gaagcgggtt caaacgcccg aaccagcgat attagcagcg gagaatatgc cgttatgcca
    16741 ctggcaccaa tgaaggagtc cgatgcgccg aacgaagaac tgcgtcaggc ctgggagtat
    16801 taccacaccc ctcgcgcgca gtatccaaca gcaccgggtt acgctacttt gcgcagcctt
    16861 aaccagatta ttacctatga tgcttaccat atggcggaag tgtacctgac tcagccgatg
    16921 cagattgtgg cgggcagcca ggcagggagc aaatggatga gtgacgacct gtacgatcgg
    16981 gcatcaagcc aggataaacg ctatcacatt gttgaaggcg caaaccatat ggatctttat
    17041 gacggcaaag cctatgtggc tgaagcaatt tcagtattag ctccgttctt tgaggaaact
    17101 ctgtaatgaa acatattcaa atccgcaata gtgatatggc ctggcatatt gctgccaata
    17161 ttcagtttcc accaaatttt gatgagtcaa aacagtatcc ggcgattatc agcgtccatc
    17221 cttttggcag ttgtaaggag cagacgtctg gcaatatcta tggcaaagca ttggctgaaa
    17281 aaggctatct cgttctcgct tatgacgcca gtttccaggg agaatctggt ggggagcctc
    17341 gctggataga agatccgaca cagcgcgtag aggatattag ccgtgttatc gattatgcag
    17401 taactctgcc atatgttaat gctgaacgta ttggcgtgct gggagtgtgt gggggtggtg
    17461 gctatgccat taatgccgcg ctgacagaaa aacgaattaa agcagtagtg agtattacgg
    17521 gtgttaatat cgggcgtctt tttcgggaag gtttcagcaa ttacgatccg attggcgcat
    17581 tgaatgcaat ggcgtcgcaa cgaaccaaag aggctcgtgg tggagaatta cagatcaacg
    17641 aattacttcc cgccagcctg gatgcagcaa aagcccatgg actgaccgaa cgcgatgttt
    17701 atgaagcaac tgactactat aaaacaccac gcgggcaaca accgggaggt gcgacaaaaa
    17761 tgttgttctc acatgcacag aaaacgctgg cctgggatgc cttcgcattt actgaagtgt
    17821 tactgactca gccggtgatg gtagtagttg gtgaaaaagt cggtgccttc ggtgcttatc
    17881 gtgatggact ggaagtctat ggccgggcga tggtttcgca ggatcgtcag ttagtttcat
    17941 tacccgattt ctcacattat gaactgtatg acaaaccgga agcagttcag gaggcgctgg
    18001 aaaaagttat tccttttttc aacacccatc tgggataagt ttcatgagaa atattatgaa
    18061 aaatgtactg attcttggtg ctggcggtca gatagcccgc catgtgatta atcaactggc
    18121 agataaacaa accattaaac aaactttatt tgcccgtcag ccagcaaaaa tccacaaacc
    18181 ctatccaact aatagcaaga ttattatggg agatgtactg aatcatgcag cactgaagca
    18241 ggccatgcag ggacaggacg ttgtgtatgc caaccttacg ggggaagatt tagatattca
    18301 ggcaaattca gtgatcgctg cgatgaaagc ctgtgatgtt aaacgtctga tctttgttct
    18361 ttccctcggt atttatgatg aagttccagg aaaatttggc gaatggaata atgcagttat
    18421 tggtgagccc ctaaaaccat tccgcagggc tgcggatgca attgaggctt ctggactgga
    18481 atatacaatt cttcgccctg cctggctgac tgatgaagat attattgatt atgagcttac
    18541 ctcacgcaat gaaccattca aaggtacgat cgtatcacgt aagagtgtcg cagcattaat
    18601 cacagatatc atcgataaac ctgagaaaca cattggtgat aatataggta tcaatcaacc
    18661 cgtgactgat ggtgacaagc cattattcat gtaaatcaat tacatagtta ccatcgtaat
    18721 gtactatgtg cagtacgatt tattaaactt attaaggtga actgatcaat ccgatgcgat
    18781 taacatgtgt agaaccagta agaataaaat acgccatgta tataacatta cgacttacat
    18841 ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac ttccggcaaa
    18901 aaagtgatac aagatcgatg acatagcgtt gaagaaaagg atttgataat ccgcaaaatt
    18961 tgcacaacac tgcctgtaat acttcctgaa ccagagacga tcatgaccgt aaaccgccca
    19021 cgcgccgaac gcggcgcatt tccgcccgga acagaacatt acggacgttc attattgggc
    19081 gcgccgttaa tctggtttcc tgcccctgca gccagtcgcg aaagtggttt gattctggct
    19141 ggcactcacg gtgatgaaaa ctcttcggtc gtcacgctct cctgtgctct gcggactttg
    19201 acaccttctt tgcgccgcca tcatgtggtg ctgtgtgtga atcctgacgg ctgccagctg
    19261 gggttacggg ccaatgctaa tggtgtggat ttaaacagaa actttccggc ggcgaactgg
    19321 aaggaaggtg aaacggttta tcgctggaac agcgccgctg aagaacgtga tgtcgtatta
    19381 ctgacaggtg acaaacctgg ctcagaacct gaaacccagg cgctgtgcca gcttatacac
    19441 cgcattcaac ctgcctgggt ggtctcattc cacgatccac tggcctgtat tgaagatccc
    19501 agacacagcg aattaggcga atggctggcc caggcgtttg aattgcctct ggtaaccagc
    19561 gtcggttatg aaacgcctgg ctctttcggc agttggtgtg cagatctcaa cttgcattgc
    19621 atcaccgcag agtttccgcc catctcctcc gacgaagcca gcgaaaaata tctctttgcg
    19681 atggcaaact tgctgcgctg gcatcctaaa gatgcaattc gcccgtcgtg aactgaagcg
    19741 ccggttccac atctaccgcc agccaggtcg gtccgtcaag atcggcgaaa ctgacctgcg
    19801 gcaccagcgg taaagcggcg ctaatggcac gagaggtaca caacatgcag cccagcatca
    19861 gactgaaacc ttgtgcacgc gcttcagtcg ccagcgccag cgcttccgtc agaccaccgg
    19921 ttttatcgag cttaatgtta accatctcat agcgcccttt cagcgccttc agattgctac
    19981 gtgtatgaca actctcatca gcacaaatcg gcaacggatg aataaaattc tccagcgccg
    20041 catcgtcctg cgcaggaagg ggttgttcaa gcatcgcaac gcctaaatcc gccaatagct
    20101 ggcaacgcgc cgccaaccct tctgcacgcc aggattcatt tgcatcaacg atcagcgtcg
    20161 catcgggcac agctgtgcga attgccacca tccgctcact aataagatgg ttatccagct
    20221 tcactttcag taatttcgcg cctgcctgcc agagtgttga tgcactattg gccatctgat
    20281 caggcgtacc gatgactacc gtctgtgcag tgataactgt ctcgggaagc gttatgccga
    20341 tcaaatcagc cagcgattgc tgctgttttc gcgcggccag atcccacaat gcacaatcca
    20401 gcgcattacg tgctgcgccg gcagggagaa ttttttgcaa ctcctcccgt gtcagccctt
    20461 tctctaattg cgacacgacg ctcataattt gcgccattac cgaggcatca ctttctccat
    20521 aacgcggata cggcgtgcat tcgccggtgc ctttaatacc ctcttcttcc agttcaacca
    20581 ccaccacgcg cgcttcactg cgacttcccc gggcaatcac aaacggggta tgtaagggcc
    20641 aggcttcctc aaatacctta acggttctca tctttactcc ttgttgtcgc ggtggtctgc
    20701 ggattaagcg caaatagcgt ttgctgtgtt attgacagtt agcataaact aggtgtgacg
    20761 ttaactatat gtaaacagga aagataatca tgtcacaaac cgttcatttc cagggcaacc
    20821 cggttacagt cgccaattcc atcccgcagg cgggtagcaa agcgcagact tttactctcg
    20881 tagcaaaaga tctgtctgac gtcaccctcg gtcagtttgc gggtaaacgc aaagtgctga
    20941 acattttccc gagtattgat accggagttt gcgccgcatc agtacgtaag tttaaccaac
    21001 tggcaaccga gatcgacaac accgttgtgc tgtgtatttc tgccgatctg ccgttcgccc
    21061 agtctcgttt ctgcggcgca gaaggtctga acaacgttat caccctctcc actttccgta
    21121 acgctgaatt cctgcaagcc tacggtgtgg caattgctga tggcccactg aaaggtctgg
    21181 cagcacgtgc cgttgtggtt attgacgaaa atgacaatgt gattttcagc cagctggtgg
    21241 atgaaatcac caccgagccg gattacgaag cagctctggc tgtactgaaa gcataattta
    21301 atatgcctga tggtgttgca ccatcaggca tattcgcgct tactcttcgt tcttcttctg
    21361 actcagacca tattcccgca acttattggc aatcgcggta tgtgaaacgc cgagacgttt
    21421 tgccagtttg cgcgtgctgg gataattgcg ataaagctgg gttaataccg agcgttcaaa
    21481 acggctggtg atttcgtcca gcgaaccttc catcgcatct tcgcccacgg ctaccgttgc
    21541 ggcgtcataa tccggcaaca aaatatcctg tggacgcagc tcataaccgt ccagttgtgt
    21601 cagtgcgcga tagatagcgt tctttaactg ccgcacattt cccggccacg cataacgcgt
    21661 aagtacagta ttcaggtcag cggccagttt cggacgcggc acgccctgct cgtcggcaaa
    21721 gcgggcgacg aacagctcag ttaacggcat gatgtcctgc ggacagtcac gtagcggcgg
    21781 cagattgagc gtcaacacgt tcagacgata atagagatct tcacggaaca tgcctttttg
    21841 caccagttcg accagattct tctgcgtagc gcaaatcacc cgcacatcga catgcacctc
    21901 atggtcttcg ccaacccgac ggaaagtgcc atcattaagg aaacgcagta atttcgcctg
    21961 catccgtggt gacatttccc ctatttcatc caacagcacc gaaccaccgt tcgcctgctc
    22021 aaagaatcct ttcttccctt ccggagcatg accaaacagt tcactctcga ccgcatcttc
    22081 cggtatagac gcacagttca gcgccaggta aggtttgccc gctctggggc ttgcctgatg
    22141 gcaggcgtag gcaaagagat ctttacctgt acctgtgtca cccgtaatca gcagcggcgc
    22201 gcttagcatc gccagtttct gcgcctgttc gacaacatgc ttcattttcg ggctgacggc
    22261 gacaatttga ctgaaggcgc tgacgtcctg ggcggcgaca ttttgcaact ggcggcccat
    22321 acgaatcgtt gatcgcaaca tcaccaccgc accggtcagg acgtgttgat cattttcatc
    22381 ctgaagataa acaggcgtaa tctccatcag gaaattctgc ccattaataa cgacatgctc
    22441 gttatgggaa tcttgcggtt cgctttccag ccaacgtaaa aaattaaagc cgttaatcaa
    22501 ttgtgcggcg gtatggttgc gcaggcgatc caatttttgc gcaaaaagct gacagctcgc
    22561 cgggttcgcc atatccactt tgcttttcat atcgacagag agcacaggtt cgggcaacgc
    22621 ctccagtaac gcgctcaacg ccagatgctc acgttcggaa ggcatccacg ggacagtacg
    22681 cacatcggta acacctgcaa tacggcgtat ttcggccatc agactgctga aactctcaaa
    22741 ctccagttca gcaaaattga ggtagattcg cccaatggga tcaatctcaa taccgcgtaa
    22801 atcaatgcct cttagcacga gtagatcgag taattcgcgg gtcagaccga gtcggtcttc
    22861 acaaaagact tccagacgca tgggaacctt cacctgaaaa aagaacaatt aatatgatga
    22921 tatgacacta tttggatagc aggaaggttt ctgtcaacaa tcattgacac aaagacggaa
    22981 aaacgggcac ttcggcgtaa agatatccgg tcagcacccg atattgcatt atttttcgct
    23041 gggcgttttg cctttttgca gcgtttcttt cacctgaccg ataagctgac gacggaaatc
    23101 cccgaggcga ggtttgtcat cgtcaatcca cggcagcggg cggcaaagct ccatagcttt
    23161 aatcccgagt cgtgccgtca gaagtccagc accaattccc tgagctgcgc gggtagacaa
    23221 acgagcagcg agatcttgcg acatccagtc catccccact tcgcgcacca gttcgctggc
    23281 tccggcaaaa gcgatattca gcaataccag cttaaacaga cgcaaacggc tgtaataccc
    23341 cagttcaatg ccatacagcg tggcgatgcg attaattaaa cgcagattgc gccaggcgat
    23401 aaacgccata tcgaccaacg ccagcgggct gaccgcaatc atcaacgttg attccgccgc
    23461 cgaacggctg atttcgcgcc gcgcctgggc atctaaaact ggctggacca aatgcgcata
    23521 caaactgacc acttcacggt cgttttgcgt ttcatggatt gaggcatacc agcgttgcag
    23581 cgctggatga gactgatcaa tacccgcctg ctgcgccagt ttttcgcaaa atgcgcggcc
    23641 tttgcccgtg ccgtggctgt gcaacaaatc gcgcgcttcg tcgcgttcat gggcgcgctg
    23701 tcgcaagcgc cataagcgcc gccactctgt taccacagaa cctacgccag cgccgataat
    23761 caatgcccca gcggcacatc cacccagcgc cacccagtcc tgagtttgcc aggcattcat
    23821 tgtccactgt acaccctgcc cgacaacgct tgcgccaaac agagccagcc cgcccatcac
    23881 cattttgcgc cacaggctgc gtttcggacg taacgctgca tccattaccg cttcaacttg
    23941 cccctcttct tcaggcgctt catcgagcgt ggccggggca aaattttgcg cctgattttc
    24001 gtcaaaggtc tgctgcgccc tgaattttgg attctgatcg acctccagcg gaccgtcgaa
    24061 atcaatacgt ggttttaacg gttcggtcat cgcaatttat ctcctattaa aaattcaagc
    24121 gcggcatcaa ggcgaatatg cggcagcggt ttgtcgacat ccatcacctg cggacgaaac
    24181 gcctcaaact ggaagccttg cttatcccag aacgcctgac cgggcaaacg agcgggaact
    24241 tcgccaggat aaacagtgag cggtgcgcca tcgctgagtc gattaccgcg tagcgccggg
    24301 attttctcac cgttaacatc aataatgccg ctggtggtcg cctgcaccga ggccagcccc
    24361 aggcaatcca tactaatccc ttcaaacgcc gcattttgcc aggcatcctg aatcagttgt
    24421 tgcagtaatg aaaccatatt ggcgtgctga tcgatggtca catggtccgc tttagtggca
    24481 gcaaacaata gcttatcgat aaccggcgaa aacaaacgcc ggaacaaggt acgctgcccg
    24541 tagtggaaac tttgcatcag ctgcgtcagt gccagacgca tatcattaaa tgcctgtggc
    24601 ccactgttga gaggttgcag gcaatcaacc agcacaatct ggcggtcaaa gcgcagaaaa
    24661 tgattcttat agaacccctt caccaccttc tcgcagtaat aattaaaccg ctcgcgcagc
    24721 attccggcat tggtgtgctt atcggcctgc gccagtttgg actcgcccca ggcatcgaca
    24781 tccggccacg ggaagaattg cagcgcgggc gcacctgcca tatctcccgg caagacaaag
    24841 cgcccaggct gaataaagtg cagcccctgc tgtttacagt ggtggagata atcggtccac
    24901 gcggcagcaa tgtctgccag ccggttttcg tcggcaggcg ctagcgggtc cagcccttcg
    24961 cacatcattc gccatttgac cgaccattcg ccgcgctgac cattgagtaa gcccgtcatc
    25021 tggcgtgacc agcttaaata gtcctgcgcc agcatcggca ggtcgagcaa ccattcgccg
    25081 ggataatcca caatctccag atacagcgtg gaggtgtcct tgaagtggcg tagcagcgaa
    25141 tcgttcgatt taaaacgtag cgccaggcgg atttcactga cgccgcgcgt tggcgtcggc
    25201 caggcgggag gatcgccata taactgcgcc agtccttcat catatgtgaa gcgcggaatg
    25261 ccaaagtcac gctgaggaat gcgttttacg cccagcaggc gctcttcacg caccgcgctt
    25321 aacagcggca agcgtgctcc ggcgtgaata ttgagcaact gattgaccat cgcagtgata
    25381 aacgctgttt tgccgctgcg gcttagtccg gttacggcga ggcgcagatg tctgtcgaca
    25441 ccccgattca ccagcgcatt aagttcattt ttaagtcgct tcattgctgt cctgtgctgc
    25501 tctgaagacc agaaaaaaat cattcgtaag gatatgaatg tgggggccag gccattgatt
    25561 atcaactgcc cgtgttgtca tcgacgggcg aactccataa gatgtgcagc attcacgttc
    25621 ggacggagtt cctgacctat gtcccctact atttatgata ttgccagggt tgcaggcgta
    25681 tcaaaatcca ccgtatcacg cgtgctgaat aagcaaacta atatctcccc ggaagcgcgc
    25741 gaaaaagtgt tacgggccat tgaagaatta cagtatcagc caaacaagct ggcccgcgcg
    25801 ctgacctctt cgggttttga tgccattatg gtgatttcta cccgttcgac caaaactacg
    25861 gcgggtaatc cgtttttctc tgaagttttg catgccatca ccgccaaagc tgaagaggaa
    25921 agtttcgacg tgatattgca gacgtcgcac aatccggcag aagacttaca aaaatgtgaa
    25981 agcaaaatta agcagaaaat gattaaaggc attattatgc tcagttcgcc agcggatgag
    26041 tcattttttg cccaactcga taaatacgat attccggtgg tggtaattgg caaagttgaa
    26101 ggtcaatatg cccatgttta ttctgtcgat accgataatt ttggcgacag cattgcgttg
    26161 accgatgcat taattgaaag tgggcatcaa aatattgcct gcctgcatgc tccgcttgat
    26221 gtccatgttt cggttgatcg cgttaatggc tataagcaaa gtctgtccgc gcataatatt
    26281 gcagtgcgtg ataaatggat tgttgacggc ggttataccc atgaaacagc actgcaagca
    26341 gcacggcaat tattaagtca gtcaccgttg cctgaggcag tgtttgccac tgacagcctg
    26401 aaattaatga gcatttatcg tgcggcggca gagaaaaata ttgctattcc gcagcagtta
    26461 gcggtggtgg gttatagtaa tgaaacgctg tcgtttattt taacgcctgc accaggcggc
    26521 atcgatgttc cgacgcagga gttaggtcaa caaagctgcg agttattatt ccgcttaatt
    26581 tctggaaaac cgtcaccaca gaatattacc gttgccacgc atatgacgct gaaataaata
    26641 ccagctacct tgcctgaatg ccggatgcga tgctagcgca tcttatccgg cctacattta
    26701 tagatcggat aaagtactag cgccgcatcc ggcaatttag cccattatca gaacgagtaa
    26761 tttacgccga cacctgcata atggaattta tcgctgtcgc cttcgtcgtg atcctgccac
    26821 tcaaacgcgt attccagcga aacggaaaga ccattctgga aatcataacc gtaaaataaa
    26881 cccacacggt taaaatcatg gccttcacgt tcgatatcat cctgccagtc ccagttactc
    26941 cagcgatcca gcccaatgcg cgtatacggc gtcaccgagt ggttgccgag cgtcagcggc
    27001 aaataggcgc gaatttcttg cgtagaaaac tcaccgttat tgcggctgtc gtccatatta
    27061 aagccgcgct cgagataata gttcactcgc aaggcaaccg tttcgttgaa ggtatattgc
    27121 aggcctgttt ccgtttcgac tcgggtatca gcgtaaccgg tcgtgttcag atcgttggca
    27181 aatttataca tcgacaacca accgttgaaa cgtaaatcgt cagtcagttt cacatcccag
    27241 tctggcgcga ttttccagcg ctgcatattc gccgtgtctt tacccggttc atcaacgtag
    27301 tgataaccat aattacggaa accgccggtc aggccgaaac tgaaatcatc gttttcgagg
    27361 aactgataat gcacctccag ctccgggcga tcaaaccacg ttccacgttt acccgcgcta
    27421 taatctaccg gcccttcctg ataataggcc agagcaattc tccacggccc gttggcggca
    27481 ttaaaataga ctgaaggctc cgccagccca tccatatctt cgccataacc ctcgacgttt
    27541 tctatttcgt acatcgcgcc gatattaaag tgccagtcgt tcctttcctc ggcctgtgcg
    27601 caggccattc ccgcacacat caccagtgcg gtacagggta ataacttttt cattattgtt
    27661 ccttgtatta ttctggggga atgccttccc cctgtattta gcgaattgct atttccgttt
    27721 ctgcatcaaa gaaatgacat ttcgtcatat caaaatgaat agtgatattt tctcctgcat
    27781 gataatcatt taacgcacca gcacggacca ctaactcgtg ccccccaacc gtggtgtaga
    27841 gcataaattc cgcaccggtt aattctgcca cgctaatttt ggcggaaatg ttattttcct
    27901 cttgcgcgtc cggatgaata tcttccggac gtattcccat cacgatgggc ttatgcaaac
    27961 ttccctgtgt tttcagaacc gctaatttct cttcgggaat ggttaattta agcgtttccg
    28021 taacgaattt atcgccatcg atcgtgccac gaataaaatt catcgccggt gatccaataa
    28081 atccggcaac aaacatattc gctggttggt tataaaccgt tttcggcgca ccaacttgct
    28141 gaacaatccc atccttcata atcacaatcc gcgtcgccat ggtcatcgct tcggtctgat
    28201 cgtgggtcac gtagatcatg gtggtattca gcttctggtg tagcttgctg atctctgcgc
    28261 gcatttgcac gcgcagcttg gcatcaaggt tagagagcgg ttcatccatt aaaaacacgc
    28321 ccgcttcgcg cacaatcgcc cgcccgagcg ccacacgctg gcgttgcccg ccggaaagcg
    28381 cgcccggctt gcgtttcagg tactcacgca ggccgagaat ttgcgccgcc cagttcaccc
    28441 gctcatcaat cacctctttg gcgatttttt gcatcttcag gccaaacgcc atgttgtcgt
    28501 aaaccgtcat atgcggatac aacgcgtagt tctggaacac catcgctata ttgcgtgctt
    28561 tggctggaac gtcattcatt cgtttgccgt cgatcaacag atcgccgccg ctgatctcct
    28621 caagcccggc aatcatgcgc agggtggtcg acttaccgca gcccgacggg ccgacaaaca
    28681 cgatgaactc tttatcggca atttccaggt tgaagtcctt caccacatgc acctggttat
    28741 cgtagatttt ttgaatatgt tgtaacgaaa gctgagccat gttgattcct ttgctatacg
    28801 ttttgccaga aggccgataa ccgcgaccag gtcagagagt ccgttgaagg caacagtaat
    28861 tgcgccccgg ttaagcccgc gccgatcccc accgagcgca taccgctggc gttaatggcg
    28921 tcaatgcccg cctgcgcatc ttcaatgccg atacatgcct gcggcggcac gcccagcccg
    28981 gcacaggcag cgagaaagat ttccggatcc ggtttcgagt ttttaagctg ggaagcatcc
    29041 gcgcagaagg tgaaaaactc gcgcagctcc agcgccgcta aaatcgtcgg cgcattcagg
    29101 gagacggaag ccagccctac cggaatctgc tgtgcacgga gctctgccag caaattgcga
    29161 ataccgggca gcacagcatt aaccgtcagc tcgcgcagtg agtggacgta aagcagattt
    29221 ttgcgatacg ccagttgcgc ccgctcctgc gggttaaagt cgccctcttt gcccccgtgt
    29281 tgcagaatgc gccgcagaga ctcatcgcgg ctgatccctt ttagggattc gttaaactgc
    29341 gcatcaatgc tgatgccaat ttcagcggca atctgctgcc acgccaggaa atgcagatgc
    29401 gcggtatcgg tgattacacc atccagatcg aaaattaccc cttgcagttt cattcaccct
    29461 cctgatgcgt ggtagcggtc ccattgaagg gcaaaataaa atcacccaaa cagaaaacag
    29521 attcttcagc cacggatata agctgaccgt tcaaacgcag tgaaacgggc gcagaagttc
    29581 gaatcgcaat acgctgcgcg tcgagagtga cctgtaattc gcagccctgc cagaacagag
    29641 ggaaagacaa ctgttgccac tgctcaggta acgccggatt gagatgcaat tcaccgtcac
    29701 gcacgctcac cccggcaaaa ccctgaatcg cccccagcca gatagcgcca gttgcggcag
    29761 catggatacc atcatcacaa ctatgcggat cagcaccaag atcgatttca gtcccctcgc
    29821 gccagaactg ataactttgg gtcagcaggc cacagcgtgc ggcaacaatg ccgtggattg
    29881 ctttacttaa tgacgagtcg tgaatagtgc gcggttcata aaattgcaga ttggcaagac
    29941 acgatgccgc tgagaactgc tccggcagca tgtaattgag catcaccaca tctgcctgct
    30001 tgaggatctg catctcgttc acttctgcgc gtgaataatc cagcagtatg gtttgcttcc
    30061 ccgccgccgc tttgtatttc gccaggttaa tcgccggctt agccataaac gaatcatcct
    30121 gcggcaaaac gccgtcgggc tgaatttctg gcagccgcag ttctttaagg aacatttcgg
    30181 cacgatgaat aaacgcgtcg tcgctacagc caaactggcg ggcaatactc agcgcctgct
    30241 ggacgttgta atacgccata tagctggtga aggcgttatt attgacatgt tcggtatatt
    30301 cgtcaggccc aataacatca tgaatttcca gacggtcgtt aaccctcacc gcgcggctaa
    30361 tccagaactt tgcagtttcc agaagtagcg ccatgccttc atgcgcaatg aaactttcat
    30421 cccccgtagt ctgccagtat tgaataaccg cccaggcgat atcggccacc agatgatgtt
    30481 ccgcctgcgc cgaggccact ttttgccgca gcccggtgcg aatgttaatg gcggcaaaaa
    30541 gtggcgtctc ttcttcgccg ctgcgcgcgc tttcccacgg gaatagcgcg ccctgccagc
    30601 cattgcgtcg cgctttctcc tgcgcgccag gcaagttgtg ccagcgataa cgcagtaaac
    30661 ttctggcaat cgtcggatcg ctaaacagat ggaacggcag caaaaatact tccgtatccc
    30721 agaaaacgtg gcctttgtag ccttcgccgg ttaagccttt tgccgcaatg ctgctacgct
    30781 cgtcgtgagc aggcgtcatg atgcgcagat gataaagcgc ataatctaac gcttgctgat
    30841 cgtgcgcatc gccgccgttt accgtgatac gacgtttctg ccaccactga cgccagtttt
    30901 ctgttgatgc tgcaagaagt tggtcgtaac tctgctgcgc gcacatttca agctggcgaa
    30961 gcgacgcgct gccccactcg tctaaaacgg cttgcctgtc atcccgccag tcgatccaca
    31021 ccagtttttg caacgtcact gtctcgcctg catgaagctg cgcactggta tgttgcagta
    31081 aacggcgctc tttggcggta tagcatttct gcacatcacc gctcacctta cagcaacagc
    31141 tgatggccac atcactgcgt ccatcctggg tggtgtagat cccctgcatc aaatgctgac
    31201 caaacacccg cacctgggtt tcgtcgagat gttggcgacc gtggttggtt tgcgtggcgt
    31261 cgatacctgt tgaaatcagc actgacgcgt cggcgtccag tggcgtaata gtgatttcca
    31321 gcgcaatgag cggcagttgg tctgccgaaa caaagcgacg gctggcgatg gtgtaacctg
    31381 cgccgttgct ggtacgccag acaacgttgc ggcgtaattc gccactggca aagtcaagct
    31441 cacgctgcca ggcttcgtgg gataacgaga aaacctcacc atttatggca atctccatcc
    31501 ccaagatatc aggcaggttc accagttcgt tgatttcacc tttacccgcc cgatgataca
    31561 gccccgccag atacatccct cgcgtctggc gcgtgtaatc ttcttcatgg ctggcgcgaa
    31621 gcccaagata gccgttcccc tgcgccatca gcgatgcata cttgttcagg gtatgctggc
    31681 taaaatgggg ttctgataac gttactggcc tggtcataat tccacacgcg tccctgtttc
    31741 tgcggattga tataacgccg ccaccagttg ctggatgatg tacccctgct cggcgtcggc
    31801 tatcatcacg ggcttgccct gtacgtgatt gataaaggct tccatgctgc gcaaatggcg
    31861 gttgtcgtct gccatttccc gttgcatcag cgtcattaat tcaccgttgt tatcggtgta
    31921 gatatgtgct ggaaacagcg tcgcaccggc tttatcacca caaaagctga cgttcataat
    31981 cgactgttcg cggatgttga gtgcaaatga cgtttccagc cacaggatgc cgccgttatg
    32041 aaattcaatg gtgccaaaca gcgaatcttc gacgctgtaa gttgccggat cccactcgcc
    32101 aaattgacca cagctctttt gcgtgccgat cttttgaaag ctatgcccat tcacgctttt
    32161 caccgccgga aagcccagca catacatcgc agcatccagc atatgaatgc cgatgtcgat
    32221 cagcgggcca ccaccctgca gttctttatt ggtaaagaca ccccagccgg gaacgccgca
    32281 gcgacgcagg gcgcgggcgg tggtaacgta aatctctccc aaaacgccgt tggtcacctg
    32341 ttcacgcagc tgttgcgtat cgagcgcaaa acgatggtga aagtcgtagg ccagcacctt
    32401 gccctgtttg cgcgcggtat cgcacatttc ccgcgcctgt tctggcgtca tggcgggcgg
    32461 tttttcgcac atcacatggc agcccgcttc cagtgccatc agggtatgtt cgtaatgaaa
    32521 acggttaggt gagcagacgc taaccacatc aggtttcacc gccagcagca tggcctgcgg
    32581 atcgtcccac acggatgcat taccgtattt atctgccagc gcctgcgcct gggaaaggcg
    32641 gctatcacag acagccacca gttccagatc gttgcgggtg cagtagtacg aagcatgaac
    32701 cttatccgcc acctggcctg cgcctattat cgcgacccgc agcggagagc ttgtcattgc
    32761 acttttcaca aagacctctt agcaggtacg caaccaggcc aacgaatcac ggtacgcctg
    32821 ggcaggatct tctgcccgga tacgcccttc atacaccaca taaccctgat agttatcggc
    32881 gcgcagctgt tcaaacagcg cgtggaaatc gagggtgcca ctgcccggct ggtaacgatg
    32941 gttatcggca atatgcacat gaccgagcaa gtcacggttg tcgtgcagcg cctgcgccag
    33001 gttatcttct tcgatgttca tgtgatagaa atcgccgata atctgcacat gtttaagatt
    33061 gttttcgacg atgtaacgac gagcatcggc gagggtgttg atcatatgat cctgatagcg
    33121 gtttaacggt tcgagataca ccacggttcc ggtacgcgcg gcgacctgtt ccagtacgcg
    33181 cagggaatca ctcaccattt tgcggtcgcc gtccaggcta cgcggcgagg tcatcggcgg
    33241 taagcggaag gtaaacatgc cccacgctgc cggaacgacg atgcctttac cccccacttc
    33301 tgccagcgct tcgagaatgc gttcgatctg ctttaagcca ttaagacgac gctttcgata
    33361 aagtcaccaa tccagccgtc atagccaccg caggcggtgg tcaccggtaa accagtttct
    33421 ttgatcgccg ctttgacttc ttcgagattg ttgaccagca gtttgccgtc gatctcaaaa
    33481 ccatcgaaac ccatctcttt gatataacga aatttctcca gaatattttc cggaaagaac
    33541 gcctgattct gtgtgcctat tttcattttt ttcattcctt tgaattaaaa cgtaacgccc
    33601 attttgatgc tctgttccgg atgctggtcg acatactgca tgtagctttc cgggctattg
    33661 gcaaaggtca ccaccgggtc gattagatct tcgcaattga gataaccgtt catcagcagt
    33721 tcccagcagg tttcttcaat acgcttacgg ctccagcgcg gataatccgg gttcggttcg
    33781 ctgcacgcgc gggagaaaac aattttggcg ttattgaaat gcgcttcgcg tccgaggtta
    33841 aaaccttcag caaatggttt ggcaaacgcg acataagaga tggtgccgcc ataagcaagg
    33901 ccacgcagcg ccgattgcag cgcgtcggcg tgaccgctgg tttcgataat cacatcggca
    33961 ccctgcttgc cggtcagcgt tttgatctct ttacctacgt cagtgccgat ggggttaagg
    34021 cagaaatccg caccgtggcg acgggcaata tcacagcgat gggcgatagg atcgacgcca
    34081 attaccaccg atgcgccagc gcgtttcgcc agttggatgg ctatttgacc aatcgcgcca
    34141 agccctacca ccaccacaaa atcacccacg cgcacgttgg catcgcgcac gccgctcatg
    34201 gcaaactgcg ccgggtcgta acagacggcg tttttccagg agctgccttg cggcattttg
    34261 cgcagcttgt agttattcac cgcgttaatg atgaccgtct cggagagtgg gccgtagccg
    34321 catacgctgt cgcccaccgc gtagtcggtt acgtcgctgc cgcactcgat aatgtcgcca
    34381 accaccatgt tgccaagctg gaatttgcca aactcaatgc cgcgcggcgc atctgccgga
    34441 cgcggagtga acatctgcca ttcaccgtta aagtcttcat cgataaacgg gctggcggcg
    34501 cggaagtcga ccacttccgt tccgtgtttc ggtgcgccga aacgcacgcg gatcttaact
    34561 tcattggcta aaatcgcccg atcttcatac tcaaccagcg cagcaacgcg cggtgctgtg
    34621 gctactaact ttttcattta aaactccttg taaagaaact ggccgcttta gcccttcacg
    34681 ccaccggcgg tcaaaccact cttaataaaa cgttccgaaa gcgcatacat aatcaccacc
    34741 ggcaatgcgg tcaccagtga ggccgccatc atccgtcccc agatgtaatc tggcgtactg
    34801 aacagtgcgt tcaggcccac cggtaaggtg aaattgctgg cgctggaaag gaaaatcgac
    34861 gcaaacagat agtcgttcca cgccaccata aagcagtaga cgaacaccga tatcagcccg
    34921 gacatcgcca ggggcacggt gatacggaag atgatttgca gacggttgag gccatccatc
    34981 atcgccgctt cttcgatctc atcagggatg gtgtcgaagt agcttttcag cataaacacg
    35041 gcggtgggca gggtttgcgt caccatggta atgatcagcg ccatctcggt gtcgtaaatg
    35101 cccagcgcgg tgatgatttt gaacagcggc accaccagca aaatgccaga gaacatatac
    35161 accgtgtaaa agctggcatt gatggtcatc cgacctttaa agcgcaggcg ggaaagcgcg
    35221 taagccccaa gaatgccgag gaataccgcc accacggatg aaaccaccga caccaccaga
    35281 ctgttacgga agtagtcgac aaacgggaaa atcatcgggt taaaaatgtc gacgtaatgc
    35341 tccagcgtcc actgctgcgg cagcagcgta ggatgcagtg agatcgcctc tttcgcgccc
    35401 ttgaacgagg tcatcagcat cacaaaaaat ggaaacagcg tgatgatgag aaacagcgcc
    35461 agcccgcagt aaaaaccgat gcgactgagt gtgcgtttat ttgttgccat tgaggttcac
    35521 ccgtttcctg gtcagcagaa tgacagcgaa aataatgatg aagagcacca ccgagatcgc
    35581 cgccgctttt cctaaatcat taaaggcaaa ggcggttttg tagagatata caccgagaat
    35641 atcgaccttg gtcgtcagca aataaacatc cgcgaacatg tagaacatcc agatggtgcg
    35701 cagggtcacc accgtcgcca ggaccggcat aatcgcgggc agcgtgacga tacgaaaccg
    35761 ttgccaggca ttagcgccat ccatctctgc cgcttcgtac agcgatttgt cgatggtctg
    35821 caaaatcgcc agaaacgaga taaaggcata cgggaagtag cgccagatgg cgaacagcac
    35881 caccagcaca aagctactgc ccggattatc gaaccacagc ggtgcctgct catagagatg
    35941 cagaagatcg acgccgaggt agttcacaat gccgtagccg ttgttgaaca tgtatttcca
    36001 ggcaaacacc agcgaaatgg acggcgttac gtaggagagg atcaccagcg aacgcgcggt
    36061 tttgcgcagg cggaactcgc ggttgaaaaa catcgccact gccagcccga gcacggtgct
    36121 gcccgccacc accagcgcgg tataccagac cgtcatccac agcgagtgcc agaagccggg
    36181 atcggagagg atacgcacat agttactcac cccaacaaag gttgactcga tgttgggatt
    36241 gagcggcaga cgtaaaaaac tgatttcgat attcgacacc attggccagg ccaccagacc
    36301 acccagcagt aataagctgg gcgcgagaag cagcagcgca aagggcatat cggaacgacc
    36361 tgaaaaaagc ctgttcattg cggattcctg tcctgggttt agtgctgttc gatcagttca
    36421 tccagctttt tctggctcgc ttgcagcgtg ccagggagat cggctttacc cacggtgacg
    36481 ttatgcacca tggagctcac cacgccagaa cccgtcacat cacccatgcg ggtaaagttt
    36541 ttatccccta ctgcaccaaa gacctgaata tttggcagtt caccgattag ctgattcggt
    36601 agttcaccca gcgccttaat aacgtcgttg tctttccagg tggcggtagt gacaaccgct
    36661 ttattcaccg gcagcgcagc acttggcgac atcatcaccc agtcggcaat gttgtctgcc
    36721 tgctccataa aggtgacaaa cttttcagct gcttccgtct cttcggtctt ttgcccggcg
    36781 gtaatggtca gcgaggtcaa catgccgtag accgcagagt ttttctcggt tggcaccacg
    36841 aaaccgacgt ttttcgggtc gccttcttta atcacagccg gaaggatata ggtggagtaa
    36901 atcgccatcg gcgcggtgcc gttcataaag gcgtctttca cttccatgat gtcgttagaa
    36961 cccggcatag tgttggcagc aaggtcgcgg taataggtca gtgcctgcat catctctggt
    37021 gtatcaaggg tgattttgcc ttcggcgtta aagacgttag cttggttgga taacgcaaac
    37081 tgggagaagg attgttccgt caacacgctt tctgctgtag gcagcgcaat gccgtatttt
    37141 ttattcgccg ggtcattcag tttctgtgca acgtccagca gctgttgcca gtttttcggc
    37201 tcctcaagcc ccgcttttgc cagcacatct ttgcgatacc agataccgcc aatccaggcg
    37261 ctgacaggaa caccggtcca tgcgctacca tcttcggtac gcacaatacg cagtacgcca
    37321 tcgtaaaacg cgccttcacc aacgttgctg atgactgtgg caaccgcttt gcgatcgata
    37381 agctgctctt tgtccatcac tttggcgtag tcatggctgg tttctatcac ttccggcagc
    37441 gaaccgctac gtgaaagagt aatgacttta gtgttatagg catcttcttc cacgggcact
    37501 tgcttaacgg tgatgccagg gttttctttt tcaaaacggg caatcaattt actgataacg
    37561 gcctggcgct cctgctccac cgaagaatgc ataaattcga tggatacatt cgttttattt
    37621 tcttctttac agcctgaaat cagggcgcag gaaaccagtg ctgataacag cacgattttt
    37681 gatttaatca tgagatgttc ctttttattt aatccacata acctgccagg ggcgcagcgt
    37741 tatttcatta cccaatatat caacttcact aatcagatcc cgaccatgca gattagtaat
    37801 atttacatgc tgaatatttt tactgacatt aaacaatcca gtcagacaat taccatcagc
    37861 gttacttctt tgaatacgca ttacggatga attaatcgta tcgatggtaa aattattatc
    37921 cggatgaaac tcgttatggc tgcgacgaag tgtaattaaa cgcgacaact catgatatac
    37981 cgcatgcctt aatgtagctt catcgttcag ttctcgggtt atctctttac tgtgatattt
    38041 tttacggtta atcgcacggt tatatccgag tttttcgaca cctgcgtaat cattacgcga
    38101 gccaagaata ctttgaatat atatcgctgg cacaccgggg aaacttaaca aaatcgcatg
    38161 ggcgagaata aacctggcgc aacgttcttc atcgctactc tcacggcggc ttaacgcatc
    38221 catataggtc acatttattt catacggact gcgtgtaccg tcgggattat ttttccagtt
    38281 tactaatgca ccttcctgct gtaacgcctc gaccagctct aatatttcgc tttcaggcaa
    38341 caagccccgt agcgggttta gcccgatgcc atcgtgagag gcgaggaagt taaaccaggt
    38401 ggtgttgctg gaaggtagtg tcaggttttg cgcccacgca caaagcgcct caacgttctg
    38461 tttttgcacc gcatgcagca ccagtggcgg cagcgagaac tggtacacca tatgtgcttc
    38521 gtcatcgcct gcgccaaagt aagcaatgtt gtctttatgc ggaacattgg tctcggtaat
    38581 gatcactgta cctggcgcaa cgttatcaat aatcgaccgt aacagtttga taatcagatg
    38641 tgttttttcc agatggatgc agcttgttcc cggctctttc cacataaagc caacggcatc
    38701 caggcggaca tactcagcac ctttcgcaag gtaacagagt aaaacatcca ccatcgccag
    38761 caacacttca gggctacggt aattcaggtc aatttgatcg tcactaaagg tggtccataa
    38821 atggcgcgtt gaatgatcgc gcatctggaa tggcgttaat aacggtaacg cacgcgggcg
    38881 agtgacggcg ctgagatcgg tttgcgggtc aacggcaata aaaaaatctt caaaacctgg
    38941 atgctgttgt aaatagtttt taaaccattc actttttgcc gacatatggt tgcagacaaa
    39001 atcaaacatt aaatgactgc attcaccgag ttgctgaata tcctgccact cccccgcttc
    39061 actggcgacc tgatgataat caattaccga aaagccatca tcagatgacc atggataaaa
    39121 cggcaacaaa tgaacatgtg aaaaaatgct ttgcagccat tgatggtaaa actgattaaa
    39181 tgtgggtaat ggttttaaat cattgctgtg aaattgatcg gcataggtaa ttaacacgac
    39241 atcactttca ttccagtgtt ttttacgtcg ctgtgtaatt aatcgtttcg cactctcaag
    39301 acgcgttaca agtttctgta aatgagttgc ggtaaatgtt ccaccgtaga tttcgtccag
    39361 gtaatccgta attttctgtt tcatgttgcc tttccgtggt agcggtccca tgaaacgaga
    39421 gactactccg cagaaaataa actgtcaatt gagcacaagg gttttagtca cgcaggaaat
    39481 cattttttac cagggaaaaa gcgtatttgt gagctgacgc aaataatcta tggggatgta
    39541 aataaggtgt gacagaaaaa aaacggcgca taagcgccgc tcatggtgaa ttcttttaac
    39601 ctttgacctt cggcattgcg atgtctttca ggccaccggc gttctcaacg tgggtatatc
    39661 ccatctcgct aaggatctct tttgcttgcc ctgactggcg tccggcattg caatacactt
    39721 tcacggtgtc gtttttatcc ggaacggcgg tggcaatgcg ctctttcact tccttcaggg
    39781 gaatattgat ggccccctga acgtgctctt gctgatactg ctctggaaca cgaacatcga
    39841 tccagtgttc agcggcgaaa acgggcagtg aaaacaccag tgccagagca agtaagcctt
    39901 ttttaaacat gtaagcgtcc ttctatggat gaaaattgcc atccattata cgcctgagat
    39961 ttaacaattt ttgcgaaact cccctcacct tttataacgc tgtgccagtt tattagcagc
    40021 ccgacttaac agcggttcca gcgccacagc cagcaacatt ttcagcggtc ggcgagcaac
    40081 tgattttatc gcccagcccg ctacacccgc cgggccatag cgcagtgcgg taagaagtac
    40141 cagcttgcct gctaatttga aaccaggctt taccttttgc ccggcctgtt gccagcgagt
    40201 attcataact ttcctcacag ttgacggaaa cggctacgta acgtgaaagt atcggaagtg
    40261 acataacgtt ccatctcgcg taaacgcgtt tcacttgccg ccagttcacg gtcgacttca
    40321 tccagcaatt cgctgctgga aggtagctgc tcaccaaagg ccatgttgtc cggcattgga
    40381 tcaagcgcaa atgacaaaat gatgtaagca accagggtaa acagcgccag accgaagaaa
    40441 atcgacagca ccaccaggat acgcaccagt tttgccggta catcaaaata gttggcaatc
    40501 ccggcgcaga cgccgcggac catgccctgc tgtggaatac gccataattt tttattgaga
    40561 ttaatgcccg ccattagcga tccctccagt tcggatgttc tgcatcaaga atagattcca
    40621 gcgcctgaat acgttcgcgc atccgttttg cttcatcagc cagttgcgct aatcgctgct
    40681 gctcactttg cgacaattca ctgcgaccag aacgattgct gtaatgcagc cataaccaga
    40741 tcggtaaaac aaacagcaca aaaatggtta acggaatagc cagaaatagc gcgctcataa
    40801 gtactcctta gacgatgagc ggcggcgcgt tcggacgccg cctggatgtt attattgatt
    40861 gtcttgcttc attttggctt ttaattgtgc cagttgttcg ctgattgcat catcggcttt
    40921 cagttcggca aactgatcgt ccagcgattt ttgtttaccg aagctgtggc tttctgcttc
    40981 cgcttccatc tggtcaatac gacgttcgaa agattcgaaa cgagccattg cttcatccag
    41041 tttgccactg tccagctgac gacgcacatc gcgcgacgag tttgccgcct gatgacgtaa
    41101 catcaatgcc tgctggcgag cgcgtgtttc gctcaatttg ttttccagct caccgatctc
    41161 tttcttcatg cgtgccagcg tgtcgtccac cagcgtcact tcatgttcca ggctcttaat
    41221 cagatcggtc agtttctgtt tttcaattaa cgctgcacgt gccagatcct ctctctcttt
    41281 cagcagcgcc agttcggctt tttcctgcca ttcaacttca cgcgccgacg cttgttcaat
    41341 acggcgagtc agctgtttct tttctgccaa cgcgcgcgcc gaagtagaac gtacttcaac
    41401 cagtgtatct tccatctcct ggatcatcag acgaaccagt ttctgtggat cttccgcttt
    41461 ctctaacaga gcgttgatgt tggcattcac gatgtcggca aagcgagaaa aaatacccat
    41521 aatgttgtcc tcttgatttc tgcgttcagg attgtcctgc tgaactgtta atacaatttg
    41581 cgtgccaatt ttttatcttt ttgatttata aagatctgat tgaagaatca acagcaacat
    41641 gccaggatga gttagcgaat tacactaaca agtggcgaat ttcatcatgg cagaatacaa
    41701 agataattta cttggtgagg cgaacagctt tctcgaagtg ctggaacagg tttcgcatct
    41761 cgcaccgctg gacaaaccgg tgctcatcat cggcgaacgc ggcaccggta aagagctgat
    41821 tgccagccgc ctgcattatc tctcctcccg ttggcaaggg ccgtttattt cccttaactg
    41881 cgcggcgtta aatgaaaatc tgctggattc cgaactgttt ggtcacgaag cgggggcgtt
    41941 taccggtgcg caaaaacgtc atccagggag atttgaacgt gccgacggcg gtacgctatt
    42001 tcttgatgaa ctcgctacgg caccgatgat ggtgcaggag aaattattgc gcgtgattga
    42061 gtacggtgaa ctggagcgcg ttggcggcag ccagccattg caggtgaatg tgcggttggt
    42121 atgcgcgacg aatgccgatc tcccggcgat ggtcaatgaa ggcacttttc gcgctgacct
    42181 gctcgaccga ctggcttttg atgttgtaca actgccacca ctgcgcgagc gcgaaagcga
    42241 cataatgttg atggcagaac actttgccat ccagatgtgt cgggaaatca agctgcctct
    42301 gttcccgggg tttacggagc gcgccagaga aacattgctg aattatcgtt ggccgggaaa
    42361 tattcgtgaa ttgaaaaacg tggtggaacg ttcagtgtat cgccacggca ccagcgatta
    42421 tccgcttgat gacatcatta ttgatccctt taaacggcgt ccgcctgaag acgctatcgc
    42481 cgtttcagaa accacctcgc ttccaacact gccgctggat ttacgtgagt ttcagatgca
    42541 gcaggaaaaa gagttgctgc aactcagttt gcaacaaggg aaatataacc agaagcgcgc
    42601 ggctgaatta ctggggttaa cttatcatca gttccgcgcg ttgttgaaaa agcaccagat
    42661 ttagcgaagt tgagcaatac aacttgccgg atgcggcgtg aatgctttat tcggcataca
    42721 taatcaatac gtcgtttgca ggcctgataa gcgcagcgca tcagacatta ttgcgttggg
    42781 ctattaatcg ctcagcgcat cctgcaaaat tttcattgcc gcatcgaatt gcacatccgg
    42841 gatggtcagc ggatacagga agcgaatcac gttgccgtat gcgccacagg tcagcaggag
    42901 cagcccctgc gccagcgcgc gttgctggat tttctgtgca atcgccgctg acggcttgcc
    42961 cgtttgcgga tcgttaaact ctgccgcaat catcgacccc aggccgcgta ccgcagcaat
    43021 ggccggaacg ctttctttgg catcaatcaa cgtgtttttg agacgctggc ccagttgatt
    43081 cgcgcgttcg cagagtgatt ctttgtcgat aatattgagc accgcgtgcg cggcagccac
    43141 cgccagcggg ttcccggcgt aggtgccacc caacccgccc ggcgcgggcg cgtccataat
    43201 attcgcgtta ccgaccacgc ccgaaagcgg catcccgccc gcgaggcttt tcgccatcgt
    43261 cattaagtcc ggcttatcgg cgtaatgatc catggcaaac agtttaccgg tacgcgcaaa
    43321 gccgctttgc acttcatcgg caatcatcac aataccgtgc tcgtcgcaca ggcggcgaat
    43381 ggcggcaacc agctcttttg gcgcaacgtt gaaaccgccc tcgccctgca ccggttcgaa
    43441 aataatggcc gccacctgct tcgcttcaat gtccgattta aacaagcgtt cgatggcgtc
    43501 gagggagtcc tgtgttgaaa tgccgtgtaa atctgacgga taaggtacgt gatacaccga
    43561 accagggaac gggccgaagc cgattttgta cggcgcaact tttccggtca gcgccatggt
    43621 catatatgta cgaccgtgga agccgccgct aaacgcaatc acgccaggtc gtccggtatg
    43681 ggcgcgagca attttcaccg cgttttccac cgcttccgcg ccggtagtga agaatgcggt
    43741 tttagcctgc ccgctcactg gggcaagggc gttaattttc tccgccagcg tgacgtagct
    43801 ttcgtacggc acaatctgat acgccgtgtg ggtaaactgt tgcaattgct gctccaccgc
    43861 cgcgaccaga tcagggtggc gatgcccggt attcagcacc gcaatgcctg cggcgaaatc
    43921 gatgtattcg ttgccctcta cgtccttcag cgtagcgttt tctgccgact gggcgaagaa
    43981 gttacacatc acgccaaccc cgcgtggggt agcagaaaga cgacgctgat ggaattcatt
    44041 gttgctcatg tttcacacct tttgactctt cactgttaat cggaaattaa acgcccagac
    44101 gatcgcgcag gctgtaataa gccgcgccca tcgcggtaaa tggcacgcgc agcgtgcgcc
    44161 cgccggggaa cgggtaatgc ggcagattgg cgaaggcatc gaaacgttcg gcgtcgccgc
    44221 gcagcagttc ggcaatcaga cgcccggcaa gatgggtaca ggtgacgccg tggccgctgt
    44281 agccctgcat gtaatagatg ttggtatcaa ggcgaccgaa ctgcggcatg cgcgacaagg
    44341 tcagcaggaa gttgcccgtc cagcggtaat caattttcac gcccttcagc tgcgggaagg
    44401 ttttcagcag tttcggcacc acaaggcgct caacgtcatc cgggtcgcgc gcgccgtaga
    44461 ccacgccgcc gccgtacagc aggcggttgt cggcggtaag acggtagtaa tcaagcagat
    44521 agttacagtc ttccacacag tagtttttcg ggatcagcga acgggctaaa tcttccgaca
    44581 gccgttcggt ggtgatcacc tgcgtgccgc acggcatgct gcgtttcgcc agttccggct
    44641 ctactttatc gcccagatac gcattcccgg cgacaatcac atacttcgcc gtcacctggc
    44701 ctttggcagt gcgcacaacg gctggcgtgt tgtgctggat ctgcgtcacg gcagaaagtt
    44761 catacacgcg cccgccgttg aggcggatgg cgtccgcttc gccaatggcg aggtttagcg
    44821 ggtgaatatg cccaccgctg tgatccagca gcgcaccggt atagcgatcg ctggctactt
    44881 cacggcgaat ggcgttggcg tccagcaatt ccagctgttt attgccgtag cgttcccagt
    44941 tctctttctg ctcttcaagt gtggcgagct gtttatcatt catcgccaca aacaggccgc
    45001 cggggcggta gtcgcagtca atctgataac gtttgatacg ttcgcggatg atctcaccac
    45061 cttcgaacat catgctgccg agcatacggg cggtgtccat gccgtagctt ttttcgatca
    45121 cgtcgatgtc gcggctgtag gagttcacaa gctgcccgcc attgcgcccg cttgcgccaa
    45181 agccgatgcg tgaggcttcg agaaccacta cgtcaaagcc cgcttccgcc agatgcagcg
    45241 cggaggagag cccggtatag ccgccgccaa ccacgcaaac gtcgcaggtg attgactcat
    45301 tcagcgtgtc gaatggcgcg tatttattcg cactggcggc gtaataactg ctggtatgtt
    45361 cggtcatttc aggcctccag gcttatccag atggttttca gttcggtgaa tttttcaagg
    45421 gcatgcaggg atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc
    45481 atatcgccgt cgttgtagtt attgacgaag acggaaccgg ctttcaggcg gcggctcata
    45541 cggtgcgcgc gggagaggtc gcgcgtccat accgccgcgc caaggccgta ctggctgtcg
    45601 ttggcaagct gtagcgcctg ttcttctgat gtgaaacgcg tgaccaccag caccggaccg
    45661 aaaatctctt cgcggcttaa ggacgcattc gggtctacat ccacaaagat ggtcgggccg
    45721 atggcggcag ccagcccggc gttacggcca tccagcagca gttgcccttt gctttcgcct
    45781 tcccgaataa agctatggac cgagtcggcg tgggcgcagt cgattaaagt gcccatggtg
    45841 gttgcggggt caagcggatg gcccggctgc cagttttgcg cctgctgttt taacagggct
    45901 aagaattcat cggcaatgct ctcttccagc aacaggcgtg ttccggcgat gcacacctga
    45961 ccctggttgt agaagatgcc tgctgcggtg gagctcgccg ctttttgcag atccgaacaa
    46021 tcggcgaaaa cgatgttggc gcttttgccg cccgcttcca gccagacgcg tttcatgttg
    46081 ctctcgcccg catctttcag cagctgtttc ccggtacggg ttgaaccggt aaaggcaatg
    46141 gcgtcgatat cgttatgacg cgacagcgcc tgcccggctt catgaccaaa acccgtcacc
    46201 acgttcaaca caccatccgg caagcctgct tctttcgcca gccccgcgag acgaatcgca
    46261 ctgagcggtg atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg
    46321 ccgagtttcc agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg
    46381 ccgaccggtt cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca
    46441 tacactttgt cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca
    46501 tcacgcagac tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct
    46561 tcggcgtggg cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga
    46621 gaagagagtg accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcat cgcacggtcg
    46681 atatcgacgc tcttgccgcg ggcaattttc gccagcggtg cctgggtgac cggatcaacg
    46741 gtttcaaagg tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt
    46801 tcaatggcga gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg
    46861 actcctgttt cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc
    46921 cgggccgtat gcccgtttat ttctggcgac attctttata tacacttcat ccttcaagcc
    46981 gcctctgcct tggctgcgtt cgctcccccg aatcacttac ctgagtaagc tcatcgggat
    47041 tcgctcactt gccgccttga tgcaacttga atgattttgt gtatagattg ccgggcgcat
    47101 tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat
    47161 aattcggcaa atacctgccg acgtattact gaaactgtgc ggtatgccgg tattaatggc
    47221 ataactttgc cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc
    47281 ttccagtact gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc
    47341 cggctggtac gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag
    47401 cttcattgac acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg
    47461 cggctcatca ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt
    47521 cagcagcttt tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat
    47581 agcactgtga gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg
    47641 gcggatttcc gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc
    47701 ttaaactgta gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata
    47761 aagccctcga acaatatacg cgaaagcgcg tactcgctac tgttccattc cgggtgccac
    47821 tgtacgccca gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc
    47881 gagcgagctt caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgtagagag
    47941 tttacccaaa agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc
    48001 tgaacttcat gcgacggtgc atattgctgt tccaccggca gttcgggatc ttcccgatgt
    48061 tccagcaatt caggctgttc gcacagcttg cgatgcaacg aaccaccggt tgccaccact
    48121 agttcttgta aaccccggca gatggcgaaa atgggaatgc gcctttcgag tgcggcatta
    48181 attatcgcca tgctcagaag atcacgcccg ggatcggcgt caggctcatc gccgttttca
    48241 ccatataggt gcggctgcac attgctggga ctaccaggaa gataaatgcc atcgagtttc
    48301 ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat gtggtagcgc aattggcaag
    48361 ccgcctgcgt ggatgatggc attcaggtac ttttcttgca gagtctgggt cgcatgaccc
    48421 ttaagcctgt tcctgcacat tacgacaccg ataaccggat tgttcattat attttccatg
    48481 ctcaatctca caaagtggac taaattatcg ccattactgc gctttaaatg acctttatgt
    48541 tcaatatttt ttcaatctag cagtgagttt tcatttttgt aaactcaatt taacatttga
    48601 caaacattta gtttgcatac agattcgaat ggtggtcatt atattttacg ctttgataac
    48661 gagcggaaaa caaaccaaag gcgaagaatc atggaaacca atatcgttga agtagagaac
    48721 tttgttcagc agtcagaaga gaggcggggt agcgccttta cgcaggaagt gaaacgctac
    48781 ctggagcgct acccgaatac gcaatatgtt gatgttttac ttactgattt gaatggttgc
    48841 ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagt tggagaaagg gtgttatttc
    48901 ccggcctcgg tgtttgcaat ggatattctg ggcaacgtag tagaagaagc gggtctgggt
    48961 caggaaatgg gcgagccgga tcgtacttgt gttcctgttc tcggttcctt aaccccttct
    49021 gccgccgatc cagagtttat cggtcagatg ctcctgacca tggtcgatga agatggcgct
    49081 ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt ggcagcagct acgccagcgc
    49141 ggattgttcc cggttgtagc ggtagagctg gagttctatt tactggatcg tcagcgcgac
    49201 gctgaagggt atctgcaacc gccctgcgcg cccggcaccg atgaccgcaa tacacaaagc
    49261 caggtttact ccgttgataa cctcaaccac ttcgccgacg tgctcaatga tattgatgaa
    49321 ctggcgcagt tacagttaat tcccgccgac ggtgcggtcg ccgaagcctc gccgggtcag
    49381 tttgaaatca acctttacca taccgataac gtgctggaag cctgcgatga tgcgctggcg
    49441 ctaaaacgcc tggtgcgtct gatggcagaa aagcataaga tgcacgccac ttttatggcg
    49501 aagccgtatg aagagcacgc gggcagcggg atgcatatcc atatcagtat gcaaaataat
    49561 cgtggcgaga atgtgctttc tgacacagaa ggcgaagatt cgccgctgct gaaaaagatg
    49621 ctcgccggga tgattgacct gatgccgtcg tcgatggcgt tgctggcacc aaacgtgaac
    49681 tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc aggcgtcgta gggccataac
    49741 aaccgcaccg tcgccctgcg tattccgtgc ggcgaccgtc ataatcaccg cgtggaatat
    49801 cgcgtggcgg gtgccgatgc caacccatat ctggtgatgg cagcgatttt tgccggtatt
    49861 ttgcatggcc ttgataacga gctgccgttg caggaagaag tcgaaggcaa cgggctggaa
    49921 caggaaggct tacccttccc gattcgccag agcgatgccc tgggtgagtt tatcgagaat
    49981 gatcacctgc gccgctattt aggcgaacgg ttttgccatg tgtatcacgc ctgtaaaaat
    50041 gatgagctgt tgcagtttga gcgcctcatc acagaaacag aaattgagtg gatgttgaaa
    50101 aacgcgtaat tccccatgcg gggttgtttc atcggcctga gcggagccag gcctgacatt
    50161 tgctggtaat ccagacattt cccggatgtc gcgttatgga gcgcaggcgg caacgggctt
    50221 ccgttaacga ttaagactgt gacgaggaaa tgagatgatg ccgatgatta aatccccgca
    50281 tggcgaggga ggctgcgtat gcgcccctcc tgctactgac tggactccgc cccctttgct
    50341 tcccttgcta aacaggtttg atttcaggtt gacacgaccg caaaccttat tacgcagggg
    50401 aggcagcaat tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc
    50461 gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat gggtctggcc tatctcacgc
    50521 cgatgaccgt atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg
    50581 cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg
    50641 ttcgccagtt tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaatccgc
    50701 acgtcggatt tatggtcggc tggtcatcac tgctggatta tctctttttg ccaatgatca
    50761 acgttttgtt ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt
    50821 gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct gaagagcgtc aacctggtcg
    50881 ctaacttcaa taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc
    50941 tggtagttca gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt
    51001 ttatcagcga gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct
    51061 cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg
    51121 tgatcccgaa agccatcttc ctgacggcgg tatacggcgg cgttatcttt atcgctgcgt
    51181 cgttctttat gcagctgttc tttcctgata tcagccgttt taaagacccg gacgccgcac
    51241 tgcctgaaat tgcgctgtac gtcggcggca agctcttcca gtcgattttc ctctgcacca
    51301 cgtttgtgaa cacgttagcc tctggcctgg cttcacatgc cagcgtgtcg cgtctgctgt
    51361 atgtgatggg acgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat
    51421 ggcggacccc ggcactcaac gtcattatgg tcgggattgt cgcgttgtcg gcgctgttct
    51481 tcgatttagt taccgccaca gcgttaatta acttcggtgc tctggtggcg tttaccttcg
    51541 tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg
    51601 atcacttcca ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg
    51661 ttaacctaga gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat
    51721 atttgtggta tctgatccgc cgctatcgca aagtgccgct gtacgaaggt gacagaacgc
    51781 cggtgagcga aacgttatag ccggatgcgc ggcgcattat cctgcctgag ggtaacgcgc
    51841 cgcgccatta actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta
    51901 tgcttagcgc attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt
    51961 gatattgccg atgagtacgc gaccgaatgc gcagagcctg tcgcagaggc tgaacgcaca
    52021 ccgctggccc actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc
    52081 gaagaagccc aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag
    52141 atcgcagagt ttttgaatca atggggtaat gagtaatgat ttaattgcag acgatcggtc
    52201 agtatcgttg tccggataaa gcgttcacgc cgcatctggc taacaatgac acgcactgca
    52261 gcgtgcttta atgggtttct cacgatcgta ccgctctgct cctcgctaag ttgttctgtg
    52321 agttatagtt atagagaaat cacgggtacg aatgttgaac agatggcgac gccacctgtt
    52381 caacgaccaa ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt
    52441 cattaaatat tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta
    52501 ttatcgtctc ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa
    52561 tcccccccgc atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc
    52621 aacaccttta acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag
    52681 ttttatgatc gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc
    52741 gaaagctggg aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt
    52801 ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg
    52861 tttaccttcc agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac
    52921 ttcccctact tcgacagcct gcaatttgcc gataacgtga aaagcgtccg caaactggat
    52981 aatcataccg ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctcgca
    53041 acccattatg cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc
    53101 caggagcaac tcgaccgtca accggtcggc accggtccgt atcagttgtc ggaataccgc
    53161 gccgggcaat ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg
    53221 ccgcaggtag tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc
    53281 ggggaatgcg acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac
    53341 ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc
    53401 gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac tggcactggc gattaataac
    53461 cagcgcctga tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg
    53521 cgcgcctcgt gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg
    53581 cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca
    53641 cgttcgcagg cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg
    53701 gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg
    53761 ttgatggata tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg
    53821 gacagtttct tccgtccgtt actgagctgc gcggcaattc attcacagac caacctcgcc
    53881 cactggtgcg atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg
    53941 gcagcgcgta ttgaagccta tgacgaagcg cagagtattc tggcgcagga attacctatt
    54001 ctgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta
    54061 cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg
    54121 aaaaaaccat gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc
    54181 tgctgacctt cgttggcttc agtttgagct atttcactcc acacgcgccg ttacaaggcg
    54241 cgtcactgtg gaatgcctgg gtgttctggt ttaacggcct gatccactgg gattttggtg
    54301 tttccagcat taacggtcaa ccgattgccg aacagttaaa agaggtcttc cctgcgacga
    54361 tggaactgtg catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga
    54421 ttgccgggat tacgcgccat aagtggcagg ataacttgat caacgccatc gccttgctgg
    54481 gcttttcaat cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc
    54541 taggttggtt gcccgtttca gggcgtttcg atctgctgta cgaagtgaaa ccgattaccg
    54601 ggtttgcgtt gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga
    54661 gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag
    54721 tgatccgact gatgcgtatc agcactatcg aagtgtatga ccagaactat gttaaagcgg
    54781 cggctacccg cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt
    54841 taccgccggt tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga
    54901 tcaccgaaat ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc
    54961 agcaggatta cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg
    55021 ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt
    55081 atgccttacg atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg
    55141 cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct
    55201 gtactgtgta tttttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt
    55261 taccaattat tgccgccgtc atggtcgcgc tatggcgaag tttctttctt cctggggact
    55321 gacgacctcg gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt
    55381 ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc tggtactggg caccttcgcc
    55441 ggggcgacgc acggcctacg ctcagcggtg cttaaccata ttctggatac cttactggcg
    55501 atcccttcgc tgctgctggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac
    55561 gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg
    55621 gtgcatgacg aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg
    55681 ctgaatattc tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc
    55741 acccgcgcac tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc
    55801 gcacaactcc cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat
    55861 gtcgccccgt ggactgtcat gctgccaggt gcggcaatta tgattagcgt gttgttggtt
    55921 aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata atgccattac
    55981 tcgatattcg taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg
    56041 accgggtaag tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt
    56101 ccggcaagag tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta
    56161 ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc
    56221 gcaaactggt tggccataac gtgtcgatga ttttccagga accgcagtcg tgtcttgacc
    56281 cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc
    56341 gttggtggca gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg
    56401 ggattaaaga tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat
    56461 gtcagaaagt gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg
    56521 aaccgaccaa ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc
    56581 tcaaccaaaa cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc
    56641 aatgggcgga taaaattaac gtgctttact gcggtcaaac agtggaaacc gcgccgagta
    56701 aggagttggt gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag
    56761 acttcggcag cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac
    56821 tgctggaaca gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag
    56881 aatgcattgt gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc
    56941 cgctgaacat ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt
    57001 aaaacgttcc gctaccggac cggctggttt cgtcgtcaga ccgtagaagc ggtaaaaccc
    57061 ttgagcttta cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt
    57121 aaatccacgc tggcaaaaat gctggcggga atgatagagc ctaccagcgg cgagttattg
    57181 attgacgatc atccactgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg
    57241 atttttcagg atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aattctggat
    57301 tttccactgc gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa
    57361 acgatgcgta tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca
    57421 cccggacaaa aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt
    57481 attgccgatg aagccctcgc ttcactggat atgtcgatgc gttcgcagtt gattaatctg
    57541 atgctggagt tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga
    57601 atgatgaagc acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga
    57661 ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc
    57721 gggcactttg gcgaagcatt gacagcggat gcgtggcgta aagatcgcta attttctgaa
    57781 tcctccttcc agattatcca cctcattctg acaagagatt aaatcttgtc agaatgaggt
    57841 gatggataaa ataaaatcaa atgcgcgtga tttaagacgc aatctcactt tgcaggaacg
    57901 aaagctctgg cgatatcttc gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca
    57961 tccggtgggg agctacattc tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct
    58021 ggatggtggg cagcatgatt tggctgttgc ctatgatacc aggcgcacta gctggcttga
    58081 gtcgcagagc tggaccgtgc tgcgtttctg gcataacgag attgattgta atgaggaggc
    58141 ggtgctggag attattctgc aggaactgaa ccgccggtca ccctcaccct gaaagggcga
    58201 gggggccgac cgagccgaat agctgttgtg gtgaaaacat ggagacagtg ctggagaata
    58261 ttcggcaagg tctgaaccat cccagccatc gccataaaag ggttaggggc tgtatgagcc
    58321 tgtttgttgc tggggtaaca atatttgcac aatatggtcc cctcgcccct ctggggagag
    58381 ggttagggta aggggaaaag cgcccaccct gccgcagcct gctccggtcg gacctggcaa
    58441 ctatagctac tcacagccag gttgattata ataaccgttt atctgttcgt actgtttact
    58501 aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta
    58561 tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctatccatcg
    58621 cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga
    58681 acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc
    58741 tgcagtgcga tgttgcagaa gatgccagca tcgacaccat gttcgctgaa ctggggaaag
    58801 tttggccgaa atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg
    58861 atggtgacta tgttaacgcc gttacccgtg aaggcttcaa aattgcccac gacatcagct
    58921 cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc
    58981 tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc
    59041 tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag
    59101 gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac tctggcggcc tccggtatca
    59161 aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtaccgtta
    59221 ctattgaaga tgtgggtaac tccgcggcat tcctgtgctc cgatctctct gccggtatct
    59281 ccggtgaagt ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaat
    59341 tgaaataatc attctgttgg taaatattgg gcggcgttct gccgcccgtt atctctgtta
    59401 tacctttctg atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg
    59461 ataacgattt cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa
    59521 gccactggta tcatgaaacg caatccagta ctgcggagta tgacgttctg cctctggtcc
    59581 cggaagccgc aaaggtcagc gatccctttc tgctcgacgt gatccttgat gaagaaacgc
    59641 tggctccctt cctttcatgg ctggtccctg cgcgcgttct tgcagtggaa ttgttccctg
    59701 accagcttac cgtgacccgt tcacagacct tcaccgctta tgaacgcttg tctacggccc
    59761 tgacggttgc tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta
    59821 cgccgctccc cgggcctgat tcctccaggg aaagcaatca taggttggca caaatcacgc
    59881 aatatgcccg ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc
    59941 tgaatgacgt cggtcttact gccggggact gtgtgatcat taaccaaatc attggtttta
    60001 ttggctttca ggcgcggacc attgcgacat ttcaggctta tctcgggcac ccggtacgct
    60061 ggttacccgg tctggaaata caaaactacg ccgacgcgtc actgtttgct gatgaatcaa
    60121 tacgctggcg aagcagctat gaggtggaaa aactacctga agagtacaca aaaagttcaa
    60181 ctgcagaact ttgccaactg gccgaaacac tctctctcca ccctatttca ctttcccttc
    60241 tcgaaaggtt gttaaacagt acacgggtta atacacagcc ggataataag cttgcggcgt
    60301 tgttatgcgc gcggataaat ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa
    60361 atgaatataa aaaaatcagc gcccttgtgc gcaagggcga aaatgaaatt aaccaatggg
    60421 ctgaccgtca ttctgttgag cacgctaccg ttcaggcgat acaatggctg acccgagcac
    60481 ccgatcgctt tagcgccgcc cagttcagcc ctttactcga acacgaaaaa tcatcaacgc
    60541 agattattaa tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg
    60601 cgttgggtga gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg
    60661 ctctaatggc caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt
    60721 aaacagcaac tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa
    60781 ggctttggct tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg
    60841 aaaaaagtca tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa
    60901 tccgcagagc cagaagaact ggttgaaccg ttcctgactc gtttcgtggg taaagttcag
    60961 ggcaaaaatg accgtctggc catcgttcct gatcatccac tcttaaaaga cgccattcct
    61021 tgccgcgcag cccgtggcct gaaccacgag tttaaagaag gcgactgggc ggttgccgaa
    61081 atgcgccgtc atccgctgaa aggcgatcgt tctttctatg ctgaactgac acaatacatc
    61141 acttttggtg acgaccactt tgtaccgtgg tgggttacac ttgcgcgcca taatctggaa
    61201 aaagaagcac cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat
    61261 ctgaccgcgc tggattttgt caccatcgac agtgccagca cagaagatat ggatgacgcc
    61321 cttttcgcta aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca
    61381 accgcgtgga ttgcagaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc
    61441 aactatctgc ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc
    61501 tcactgcgcg ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat
    61561 ggcaccattg aagataatat cgaattcttt gccgccacca tcgaatccaa agcgaagctg
    61621 gtgtatgacc aggtttctga ctggctggaa aataccggtg actggcagcc tgaaagtgaa
    61681 gcaattgccg aacaagtccg tttgttagcg caaatttgcc aacgccgcgg cgagtggcgt
    61741 cataaccacg cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa
    61801 ggtgaagtgc tggatatcgt cgccgagccc cgtcgcattg ccaaccgtat cgtcgaagaa
    61861 gcgatgattg ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct cggttttggc
    61921 atctataacg tgcatatggg ctttgatccg gcaaatgccg acgcgctggc agcgttgctg
    61981 aaaacgcacg gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa
    62041 ctgcgtcgtg aactggacgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc
    62101 cagtcatttg ctgaaattag cactgaaccc ggtcctcact ttggcctcgg tctggaagca
    62161 tacgccacct ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg
    62221 aaagcggtta tcaaaggcga aactgcgacg cgcccacagg atgaaatcac tgtccaaatg
    62281 gccgagcgtc gccgtctcaa ccggatggca gaacgtgatg ttggcgactg gttatacgca
    62341 cgcttcctga aagacaaagc cgggaccgac acccgtttcg cggcggaaat tgtcgatatc
    62401 agccgtggcg gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt tattccggca
    62461 cctttcttac acgctgtgcg cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa
    62521 attaaaggtg aaacggttta caaagtaact gacgttattg acgtcaccat tgccgaagtc
    62581 cgcatggaaa cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcccat
    62641 tcctcatgga tgggccgttt atttccccgc tctaccttcg ttatatccag gcagattttt
    62701 taactgactt tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata
    62761 aaaaaaacaa tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt
    62821 aaccgcggac tccgcagtta accggaggat atgcatcatg aaaaccgtta gggagtccac
    62881 aacgttgtac aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga
    62941 tgttttgcgc ttttctacca ccgaaaccac agaacctgat cgtactttgc agttatctgc
    63001 cgaacaggct gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct
    63061 gaccgtcgat gagagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga
    63121 atgggctggc aacgcgtctg catggcatga cacaccggca gttgctcgtg atttatcaca
    63181 cgggctttcc tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga
    63241 cagccggggg aatatccaac gcttcaatcg gttatgtgaa gattacactg ggttgaaaga
    63301 acacgacgtc attggccaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc
    63361 atccaggcgc aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg
    63421 gataccaaca cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg
    63481 cagtggcaaa aacgagattt ttttaatctg ttccggcacc gacattaccg aagagcgccg
    63541 cgctcaggag cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg
    63601 taacgcaatg caggatttaa tcgatcacgc tattaatcat gcagataaca ataaagttgg
    63661 ggttgtgtat cttgatttgg ataatttcaa aaaggtcaac gacgcctatg ggcatttgtt
    63721 tggtgaccag ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca
    63781 ggtgttggcg cgtccaggtg gcgatgagtt tctggtactg gcatccaaca cctcacaaag
    63841 cgcgctggaa gcaatggcat cacgaatttt gacccgctta cggctcccct ttcgcattgg
    63901 tttaattgaa gtttatacca gctgttcagt aggtattgca ctctctcccg aacatggttc
    63961 agacagcacg gctattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg
    64021 acgaggacaa ttttgcgttt ttaccccaga aatgaatcaa cgggtatttg aatatctctg
    64081 gctggatacc aacttgcgta aagcactgga aaacgatcag ttggttattc actatcaacc
    64141 gaaaatcacc tggcgtggcg aagtgcgcag tctggaagca ctagtacgtt ggcagtcacc
    64201 tgaacgtggg ttgattccac cgttggactt catttcctac gccgaagagt cagggctaat
    64261 tgtgccttta ggccgttggg tgattctcga tgtcgtacgc caggtggcga agtggcggga
    64321 taaaggtatt aacctgcgag tggcggtaaa tatttctgca cgtcagctcg ccgatcaaac
    64381 cattttcacc cccctgaaac aggttctcca ggaactcaat tttgaatact gccctataga
    64441 tgttgaactg acagagagtt gtctgattga gaatgatgaa ctggcactgt ctgttattca
    64501 acaatttagc caactaggtg cgcaagtgca tctggatgat tttggtaccg gctactcttc
    64561 actttcgcaa ctggcgcgct ttccgatcga tgccatcaaa cttgaccagg tttttgttcg
    64621 agatattcac aaacaacctg tctcgcagtc actggtccgg gcgatcgtcg ctgtggccca
    64681 ggcattgaat cttcaggtga tcgccgaagg tgtagagagt gcaaaggaag atgctttttt
    64741 aaccaagaac gggatcaatg agcggcaagg atttttgttt gccaaaccga tgcccgccgt
    64801 cgccttcgaa cgctggtata aacgctatct gaagcgcgca taagtagatg aatatatata
    64861 cttagcgcta tatcccgtca ttatatatcg tattcctgga tgataattag ccgttgctgt
    64921 taaacacttc tcttttagcg gcaatttttg cattattcat tttccacttt tgctataagg
    64981 atgtccttga tgtctgaatt tgatgcccag cgcgttgccg aacgtattga tattgtgtta
    65041 gatatccttg tggcaggcga ttaccactct gcgatccata atctcgagat acttaaagca
    65101 gaactcttgc gccaggttgc tgaatctacg ccagacatcc cgaaggcacc gtgggagatt
    65161 taacaatcgt gccttcaggt tttcctattt gaggatgccc ggcgcgtcag tattttttta
    65221 tttagtatta taacgttata agaattacag cgatgaattc ccgacaacaa actattctac
    65281 agatggtcat tgaccagggt caggttagcg taaccgatct ggcaaaagcc actggagttt
    65341 ctgaagttac cattcgccag gatctcaaca ccctcgaaaa actgagttac ctccgccgtg
    65401 cacatggctt tgcagtttcg cttgatagtg atgacgtcga aacccgtatg atgagcaact
    65461 atacgctgaa gcgtgaactc gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca
    65521 tctttatcga aaatggtagc agcaatgccc tgctcgcccg gactctgggc gaacagaaga
    65581 aaaatgtcac tatcatcaca gtcagcagct acattgcgca tttgctgaaa gacgcgcctt
    65641 gtgaagtcat tttgctcggt ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt
    65701 tgacacgcca gtgcatccag caggtacatt tcagcaaagc atttattggt attgatggct
    65761 ggcaacctga aactggattc accggtcgcg atatgatgcg taccgatgtg gtcaatgccg
    65821 tgctggaaaa agagtgcgaa gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac
    65881 attcatactc catcggtccc gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac
    65941 gcgccagcga tctgatgcat cttgagcaca gtaaactcac cgttcacgtc gttgacattt
    66001 aaccatttcc cttctctacg gatgatttgc agtttggcaa atcatccgct ctaagatgat
    66061 tcctggttga taattaagac tatttacctg ttattaacac tctcaagata taaaattatt
    66121 atcagcgata taacaggaag tcattatcac ctgcgtgata taaccctgcg cgcgagcaga
    66181 tttcacggaa taatttcacc aggcttattc ttagctatta tagttataga gagcttactt
    66241 ccgtgaatca taaattcagg agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg
    66301 gctgttctgg caattaccct ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg
    66361 gaccgcaaca ccgcaatcgg cgcgggtgca ggggcattag gcggtgcagt actgaccgat
    66421 ggcagtacgt tgggtacatt aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc
    66481 ggtaaataat cgtaaataaa tgtcgattat cgactcgcaa ttataatacg cttgattctg
    66541 ttcagtacga aataactgaa taatacacac agtaaaatat tcgaaagcca cggatatatc
    66601 cgtggctttt tatgttaacc acctgcgagt tttaccttca tccctttcgc ttcaagtagt
    66661 gattttaata aatcacgctt atcgccctgg atttcaataa ctccatcttt aactgctccg
    66721 ccgcagccgc attttttctt cagttccgct gcaagttttg tcagttcggc atcatcgaga
    66781 tcgacaccgg taatcaggca aacgccctta cctttacgtc cactggtctg acgctgaata
    66841 cgcaccacac cgtcgccttt agggcgtaca ggggccgctt tgggctcatc aatacgtccg
    66901 gtttccgttg agtagaccag acggctgttg gaatcactca tcatgcactc cgctgtaaag
    66961 aggcgttgat cgctttcagc gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa
    67021 tcaccatata atcaacacca gccgacaacg cctgttctgg cgtcataatg cggcgctggt
    67081 caccagcttc actcccctgc ggacgaatgc ccggcgtaac cagtttgaac tcctgaccga
    67141 atacctgttt aaagcgcaca gcttcctgag cagaacacac cacaccatca aggccacatt
    67201 tttgcgtcag tgccgccaga cgttctgcat aatctgcagg tgacagtgtc atgccaagat
    67261 cgaccaggtc gctggcttcc atgctggtca acactgtcac agcaatcaaa agcggtgcat
    67321 ctttgccaaa cggaaccagt gcctcacgcg ctgcggtcat catacgcgcc ccaccagagg
    67381 catgaacatt caccatccac acgcctaagt cagctgcagc agcgacagcg tgcgcagcag
    67441 tgttaggaat atcgtggaat ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt
    67501 cgcgcacaaa ctgtggccca aacaatgtaa acatctcttt gccgaccttc agacgacaat
    67561 cgcgtgggtc gatcttgtcg acaaaggaca gcgcgtcatc acgattatga taatcaaggg
    67621 caacaaccac aggagaattc gtaacagcgc gggaagaaga tgaagcagtt aacgtcatga
    67681 ccagaccttc ttgatgatgg gcaccggaag tggcgcgaaa caggtaaacg gcgagcattc
    67741 tacccgtgga aataaaaaat taacagttgc gatttttccc ctggcaggct ttttctgcgc
    67801 cgtcgttgcc ggagcacaat ggaacatcaa taactagtat gttgtaacta aagtcggatt
    67861 tttttaaaaa ttacaggcca tcaagaccgc gaatcggttt aatggttgac caggcccgac
    67921 aagacggaca atgccagtag agggtgtatg cggtaaaacc acatttctgg cagcgataac
    67981 gaggcttact acgtaccttt tcgccaacca tgtcacgcag caccatcagg ctctctttgg
    68041 cacgcccttc ttccgcttca tttaagtggt aatccattaa cttatggaac acacgcatgg
    68101 tcggatgacg ctgaagctgg cgcgtaatat agacctgtgc ggcctcacta ccgtcgcgcg
    68161 cttcgatgat atcagcaagc atcaattcag catcggcacc ggtgttctct tccaccgcgc
    68221 gttgcaggaa ttctgcccat tcggcagttt tacccaactg ctgatagcag gtttgcaaca
    68281 tttccagcgt ttcgctgacc agttctctgt cctgggatat cacgcgttgc aaactttcga
    68341 cggctttggc gtattctcct ttcgccataa acacgcgtcc catcatgatg gatacgcggg
    68401 cgctgttttt atctgccgcc gccccttttt ttagcaaggt catggcacga tcgagatcgt
    68461 cgctggccat atgctgcagg gctaactcac agtagaaatg ggcaatttcg acgcgctgtt
    68521 tatctttacc cagcttcacc aggcgttcgg caacatcaat tgctttctgc cactcactgg
    68581 tagcctggta gatttgtagc aactgttgca gcgcgccaat gcggaagtca gtttcatcgg
    68641 tcagctgatt gaacatgtct tccgcgcggt cgtataaccc ggcggccatg taatcacgcc
    68701 ctagttgttg aatcgccaac agacgctgtt cataggtcag cgaggcgctt tccattaggg
    68761 tctgatggat gcgaatagcg cgatcaactt cgccacgcga acggaacagg tttccgagcg
    68821 taaggtgggc ttcaacggtg cctgtatcct ctttaagcat atcgagaaac aggtctaccg
    68881 ctttatcctg ttgattacta agtaggaagt taacccccgc tacgtaatca cgcgacaagc
    68941 ggttggcttc atcttgcttg ttttgttgcg cacttctgcg gcccatatac cagccatagg
    69001 cagcggctac aggcaaaagc agaaacaaca actccagcat agaaagttat tccttcgccg
    69061 ctgacgagtg cggcactaca gccacgtcag tcgcgggtga aagctggttt tccagtcgct
    69121 ttattttacg ttcagcgcgc gccagggaaa cacgaactcg cagccagaac aggccacaaa
    69181 tcaaccaacc gatagcaaac cccgcagcaa acaataccgc cagcaatgtg gaaatacggt
    69241 actccccttg cgctaacaga taattaaacg tcacctgttg atcgttctgc gcacccaacg
    69301 tgaccgaaat cacgaagatc gctaacacca gtaagaaaat gagtaaatat ttcacattac
    69361 ttcccgttat gtggttagag cgaataaatt atggtcaact taccgcaata gccctttaaa
    69421 gtaccatttt cgcgccgtct gcgaaatggc atccacgcgc attgttgatg atttaagggc
    69481 taaattcgga aaatcaacca gcgttaactt tcttgttctc gttgcgctat ttcgcgattt
    69541 tcttccgcag gtggtgttaa tggcccacaa attcgttgcg caagccacgt tgccaccgcc
    69601 accagcgccc acgaaatcaa cgtagctact accagatcgc gtggccaatg catcccgagc
    69661 agcaggcggc ttcccatgac tcccgttgcc cagaccagca agatagcaat ggttaacgtt
    69721 cgccgacgcg gccacagcaa accaacggcc agcagtgccc aactggcagc aaacatcgtg
    69781 tgaccggaag gaaaggcaaa ccccgtctct ttctgccagt gtgaacgcaa atattgtggg
    69841 atatttttct cttcagccaa ctgttctttc actaaatttc cgcgttctgc tcgctttaaa
    69901 gtgtagaact catcaaccgg aatatgatgt gttttttcca gccagataac aaaaggtcgt
    69961 ggttcctgga ctttgtcttt gatccaggat ttaacgcctt gtcccacaag gattgcggcc
    70021 gccagaatgg caaataatac aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac
    70081 cagccgaata aaatcaaatg tgtaatgacg ccccagggct gggtgacagt ttcagtaacc
    70141 caaaaagccg cttttagtag ccaactttgt tctccaggtt gccaacgcca gccagaaatc
    70201 catacggcta ctggcatgac aagcaatagt gcagctccca ctgcggtacg tctggcaatc
    70261 gaacgcatgg cctctccttt ttgataagtc ccacaatcat aactgaaaac gccagttcca
    70321 ggaaaaattg acagatttgt gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac
    70381 cccttctcgt tatggcaaaa taagccaata cagaaccagc attatctgga gaatttcatg
    70441 cagcttaaac gtgtggcaga agccaaactg ccaaccccat ggggcgattt cctgatggtg
    70501 ggatttgaag aactggcaac cggacacgat catgtcgcgc tagtctatgg cgatatttcc
    70561 gggcataccc cggtacttgc gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc
    70621 agcttgcgct gcgattgtgg cttccagctc gaagcggcat tgacgcaaat tgccgaggaa
    70681 ggccgtggta ttttgctgta tcaccgtcag gaaggtcgta acattggtct gctgaataaa
    70741 atccgcgctt acgcactgca ggatcaaggt tacgataccg tagaggctaa ccaccagtta
    70801 ggcttcgccg ctgatgagcg cgacttcact ctttgcgctg atatgttcaa actccttggc
    70861 gtcaatgaag tccgcttgtt aaccaataac ccgaaaaaag tcgaaattct gaccgaagca
    70921 gggattaata ttgttgaacg cgtaccattg attgtaggtc gtaaccccaa taacgaacat
    70981 tatctcgata ccaaagccga gaaaatgggt catttgctga acaaataacc ctctcgcatt
    71041 gtggaattaa tttgcttgcc ggaagcaaaa taaccggcaa gcaaatactt gttacttcag
    71101 catattacga atgacataat gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc
    71161 ggtgtcgata cgacaacggc agggtacgac ctcctggcta ccatccgcac gcgtaagcgt
    71221 caccggaacc gtcgcaccgg gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc
    71281 cccggttagt cctaacgttt tacgcgttac gccttgcgga aattccagcg gcaggatgcc
    71341 catgccaatt aaattcgaac ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat
    71401 tccaagcaga cgaggacctt ttgccgccca gtcacggctt gagcctgaac catactcttt
    71461 cccggcaatc accgccagcg gcgtttgttc ctgcttatag cgcatcgcag catcataaat
    71521 agagactacg tcgctgtcag gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat
    71581 ttcattacgg atgcgaatat tggcgaaggt gccgcgcatc atcacttcat ggttaccacg
    71641 ccgcgaaccg taggagttaa agtcttttcg ctcaacaccc cgaccttgta gatatcgacc
    71701 cgctgggctg tcgggcttaa tactgcccgc cggagagata tggtcagtgg tgactgaatc
    71761 ccccagcatt gcgaggatcc gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg
    71821 catttcatca aagaaaggcg ataagcgaat ataggttgag tcctcctgcc aaccgtaggt
    71881 atcggatcgt gtgacgttaa tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta
    71941 ctctttgcgg aacatttctg tggagacttg ttctaccgca cgggcaattt cttgtgccga
    72001 tggccagata tctttcagat ataccggatc gcctttacga tcatggccga taggctcaga
    72061 agccaggttg atattcatat ttcccgccag cgcataggca accaccagcg gcggcgaggc
    72121 cagccagttg gttttaacca gcggatggat acggccttca aagttacggt tgccggacag
    72181 cactgcaccg acggttaaat cgcctttttt gattgccgtt tcgataggat cgggcagcgg
    72241 cccagagtta ccaatacagg tggtacaacc gtatcccaca aggttaaacc ccagttcgtc
    72301 gagatacggt gtcaatttcg cttttgccag ataatcagaa acaactttcg agcctggtgc
    72361 cagcgacgct ttgacccacg gttgccgctt gaggccaaga gttacggctt ttttcgccag
    72421 caagcctgca gccatcagca cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc
    72481 aatgaccaca gcgccatcag gtaactgata ctgatgtccg ttcataacat aatcgaccgg
    72541 ctggcgatct ttatgcgtgg cattcacttc cagttcgtta ctggcggcaa atgcttttgg
    72601 tacatcgggc agtgcaacgc gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac
    72661 gtcattcata tccagttcta acgtactggt aaaaatcggt tcatcgcccg ggttacgcca
    72721 catgccctgc gctttggcat atttttcgac caactcaacc tgatcttcgc tgcgcccgct
    72781 taaacgcatg taatcgaggg ttacagcatc gattgggaag aagccacagg tggcaccata
    72841 ttctggcgac atattggcaa tggtggcgcg atccgccagc ggtagtgaat ccagaccatc
    72901 accataaaat tcgacgaatt tccccaccac gccatgtttg cgcagcattt gggtaacagt
    72961 gagaaccagg tctgtggcgg taataccttc acgtaattta ccggtaagtt taaagcccac
    73021 tacgtccggg ataagcatgg aaaccggctg gcctaaca
//
LOCUS       DAZGXZ010000007        68884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000007 DAZGXZ010000000
VERSION     DAZGXZ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.007"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            162..833
                     /locus_tag="ACHKJF_000568"
     CDS             162..833
                     /locus_tag="ACHKJF_000568"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP0947987.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            843..2039
                     /gene="yaiP"
                     /locus_tag="ACHKJF_000569"
     CDS             843..2039
                     /gene="yaiP"
                     /locus_tag="ACHKJF_000569"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP0947988.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2017..2598
                     /locus_tag="ACHKJF_000570"
     CDS             2017..2598
                     /locus_tag="ACHKJF_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0947989.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2600..3373
                     /gene="yaiO"
                     /locus_tag="ACHKJF_000571"
     CDS             2600..3373
                     /gene="yaiO"
                     /locus_tag="ACHKJF_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP0947990.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETENFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLTLLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3557..3832
                     /gene="frmR"
                     /locus_tag="ACHKJF_000572"
     CDS             3557..3832
                     /gene="frmR"
                     /locus_tag="ACHKJF_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP0947991.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3867..4976
                     /gene="frmA"
                     /locus_tag="ACHKJF_000573"
     CDS             3867..4976
                     /gene="frmA"
                     /locus_tag="ACHKJF_000573"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP0947992.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5069..5902
                     /gene="fghA"
                     /locus_tag="ACHKJF_000574"
     CDS             5069..5902
                     /gene="fghA"
                     /locus_tag="ACHKJF_000574"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0947993.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6127..6666)
                     /gene="yaiL"
                     /locus_tag="ACHKJF_000575"
     CDS             complement(6127..6666)
                     /gene="yaiL"
                     /locus_tag="ACHKJF_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP0947994.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6768..7979)
                     /gene="mhpT"
                     /locus_tag="ACHKJF_000576"
     CDS             complement(6768..7979)
                     /gene="mhpT"
                     /locus_tag="ACHKJF_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP0947995.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8155..9168)
                     /gene="mhpE"
                     /locus_tag="ACHKJF_000577"
                     /pseudo
     CDS             complement(8155..9168)
                     /gene="mhpE"
                     /locus_tag="ACHKJF_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
     gene            complement(9165..10115)
                     /gene="mhpF"
                     /locus_tag="ACHKJF_000578"
     CDS             complement(9165..10115)
                     /gene="mhpF"
                     /locus_tag="ACHKJF_000578"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP0947996.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10112..10921)
                     /gene="mhpD"
                     /locus_tag="ACHKJF_000579"
     CDS             complement(10112..10921)
                     /gene="mhpD"
                     /locus_tag="ACHKJF_000579"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP0947997.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10931..11797)
                     /gene="mhpC"
                     /locus_tag="ACHKJF_000580"
     CDS             complement(10931..11797)
                     /gene="mhpC"
                     /locus_tag="ACHKJF_000580"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP0947998.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11815..12759)
                     /gene="mhpB"
                     /locus_tag="ACHKJF_000581"
     CDS             complement(11815..12759)
                     /gene="mhpB"
                     /locus_tag="ACHKJF_000581"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP0947999.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12761..14425)
                     /gene="mhpA"
                     /locus_tag="ACHKJF_000582"
     CDS             complement(12761..14425)
                     /gene="mhpA"
                     /locus_tag="ACHKJF_000582"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP0948000.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGVSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14616..15449
                     /gene="mhpR"
                     /locus_tag="ACHKJF_000583"
     CDS             14616..15449
                     /gene="mhpR"
                     /locus_tag="ACHKJF_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP0948001.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15526..16608
                     /gene="lacI"
                     /locus_tag="ACHKJF_000584"
     CDS             15526..16608
                     /gene="lacI"
                     /locus_tag="ACHKJF_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP0948002.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLLINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16731..19805
                     /gene="lacZ"
                     /locus_tag="ACHKJF_000585"
     CDS             16731..19805
                     /gene="lacZ"
                     /locus_tag="ACHKJF_000585"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP0948003.1"
                     /translation="MSMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19857..21110
                     /gene="lacY"
                     /locus_tag="ACHKJF_000586"
     CDS             19857..21110
                     /gene="lacY"
                     /locus_tag="ACHKJF_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP0948004.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            complement(21189..22370)
                     /gene="cynX"
                     /locus_tag="ACHKJF_000587"
     CDS             complement(21189..22370)
                     /gene="cynX"
                     /locus_tag="ACHKJF_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP0948005.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSALLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSTREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPMQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGLLRSISGNYLMDWAFHTLCVVGLMIITLRFAPARFPQLWVKE
                     V"
     gene            complement(22376..22846)
                     /gene="cynS"
                     /locus_tag="ACHKJF_000588"
     CDS             complement(22376..22846)
                     /gene="cynS"
                     /locus_tag="ACHKJF_000588"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP0948006.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(22877..23536)
                     /gene="cynT"
                     /locus_tag="ACHKJF_000589"
     CDS             complement(22877..23536)
                     /gene="cynT"
                     /locus_tag="ACHKJF_000589"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP0948007.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALYGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            23646..24545
                     /gene="cynR"
                     /locus_tag="ACHKJF_000590"
     CDS             23646..24545
                     /gene="cynR"
                     /locus_tag="ACHKJF_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP0948008.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(24585..25868)
                     /gene="codA"
                     /locus_tag="ACHKJF_000591"
     CDS             complement(24585..25868)
                     /gene="codA"
                     /locus_tag="ACHKJF_000591"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP0948009.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(25858..27117)
                     /gene="codB"
                     /locus_tag="ACHKJF_000592"
     CDS             complement(25858..27117)
                     /gene="codB"
                     /locus_tag="ACHKJF_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP0948010.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(27354..29240)
                     /gene="prpE"
                     /locus_tag="ACHKJF_000593"
     CDS             complement(27354..29240)
                     /gene="prpE"
                     /locus_tag="ACHKJF_000593"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP0948011.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRYQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWADCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVSVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDGRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(29280..30731)
                     /gene="prpD"
                     /locus_tag="ACHKJF_000594"
     CDS             complement(29280..30731)
                     /gene="prpD"
                     /locus_tag="ACHKJF_000594"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP0948012.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(30765..31934)
                     /gene="prpC"
                     /locus_tag="ACHKJF_000595"
     CDS             complement(30765..31934)
                     /gene="prpC"
                     /locus_tag="ACHKJF_000595"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP0948013.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLNLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(31979..32869)
                     /gene="prpB"
                     /locus_tag="ACHKJF_000596"
     CDS             complement(31979..32869)
                     /gene="prpB"
                     /locus_tag="ACHKJF_000596"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP0948014.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33108..34694
                     /gene="prpR"
                     /locus_tag="ACHKJF_000597"
     CDS             33108..34694
                     /gene="prpR"
                     /locus_tag="ACHKJF_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP0948015.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFTQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            complement(34795..35070)
                     /gene="yahO"
                     /locus_tag="ACHKJF_000598"
     CDS             complement(34795..35070)
                     /gene="yahO"
                     /locus_tag="ACHKJF_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP0948016.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            35217..35888
                     /gene="yahN"
                     /locus_tag="ACHKJF_000599"
     CDS             35217..35888
                     /gene="yahN"
                     /locus_tag="ACHKJF_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP0948017.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(35905..36150)
                     /gene="yahM"
                     /locus_tag="ACHKJF_000600"
     CDS             complement(35905..36150)
                     /gene="yahM"
                     /locus_tag="ACHKJF_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP0948018.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(36562..37377)
                     /gene="yahL"
                     /locus_tag="ACHKJF_000601"
     CDS             complement(36562..37377)
                     /gene="yahL"
                     /locus_tag="ACHKJF_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP0948019.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(37620..38669)
                     /gene="yahK"
                     /locus_tag="ACHKJF_000602"
     CDS             complement(37620..38669)
                     /gene="yahK"
                     /locus_tag="ACHKJF_000602"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP0948020.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(38756..39712)
                     /locus_tag="ACHKJF_000603"
     CDS             complement(38756..39712)
                     /locus_tag="ACHKJF_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0948021.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(39709..40680)
                     /locus_tag="ACHKJF_000604"
     CDS             complement(39709..40680)
                     /locus_tag="ACHKJF_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0948022.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(40673..42157)
                     /locus_tag="ACHKJF_000605"
     CDS             complement(40673..42157)
                     /locus_tag="ACHKJF_000605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0948023.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(42206..43189)
                     /locus_tag="ACHKJF_000606"
     CDS             complement(42206..43189)
                     /locus_tag="ACHKJF_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0948024.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(43444..43854)
                     /locus_tag="ACHKJF_000607"
     CDS             complement(43444..43854)
                     /locus_tag="ACHKJF_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0948025.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44153..45535)
                     /gene="yahJ"
                     /locus_tag="ACHKJF_000608"
     CDS             complement(44153..45535)
                     /gene="yahJ"
                     /locus_tag="ACHKJF_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0948026.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(45545..46495)
                     /gene="yahI"
                     /locus_tag="ACHKJF_000609"
     CDS             complement(45545..46495)
                     /gene="yahI"
                     /locus_tag="ACHKJF_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP0948027.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(46638..48056)
                     /gene="yahG"
                     /locus_tag="ACHKJF_000610"
     CDS             complement(46638..48056)
                     /gene="yahG"
                     /locus_tag="ACHKJF_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0948028.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48056..49603)
                     /gene="fdrA"
                     /locus_tag="ACHKJF_000611"
     CDS             complement(48056..49603)
                     /gene="fdrA"
                     /locus_tag="ACHKJF_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0948029.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(49593..50456)
                     /gene="yahE"
                     /locus_tag="ACHKJF_000612"
     CDS             complement(49593..50456)
                     /gene="yahE"
                     /locus_tag="ACHKJF_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0948030.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKEPHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(50496..51101)
                     /gene="yahD"
                     /locus_tag="ACHKJF_000613"
     CDS             complement(50496..51101)
                     /gene="yahD"
                     /locus_tag="ACHKJF_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0948031.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            51359..51856
                     /gene="yahC"
                     /locus_tag="ACHKJF_000614"
     CDS             51359..51856
                     /gene="yahC"
                     /locus_tag="ACHKJF_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0948032.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFACGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            51948..52880
                     /gene="yahB"
                     /locus_tag="ACHKJF_000615"
     CDS             51948..52880
                     /gene="yahB"
                     /locus_tag="ACHKJF_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0948033.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(52922..54010)
                     /gene="pdeL"
                     /locus_tag="ACHKJF_000616"
     CDS             complement(52922..54010)
                     /gene="pdeL"
                     /locus_tag="ACHKJF_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0948034.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKESLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54152..58135)
                     /gene="ehaA"
                     /locus_tag="ACHKJF_000617"
     CDS             complement(54152..58135)
                     /gene="ehaA"
                     /locus_tag="ACHKJF_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFP0948035.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVIVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
     gene            complement(58708..60741)
                     /gene="betT"
                     /locus_tag="ACHKJF_000618"
     CDS             complement(58708..60741)
                     /gene="betT"
                     /locus_tag="ACHKJF_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP0948036.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            60870..61457
                     /gene="betI"
                     /locus_tag="ACHKJF_000619"
     CDS             60870..61457
                     /gene="betI"
                     /locus_tag="ACHKJF_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP0948037.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            61471..62943
                     /gene="betB"
                     /locus_tag="ACHKJF_000620"
     CDS             61471..62943
                     /gene="betB"
                     /locus_tag="ACHKJF_000620"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP0948038.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            62957..64627
                     /gene="betA"
                     /locus_tag="ACHKJF_000621"
     CDS             62957..64627
                     /gene="betA"
                     /locus_tag="ACHKJF_000621"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP0948039.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GVECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMAVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            64840..65505
                     /gene="ykgH"
                     /locus_tag="ACHKJF_000622"
     CDS             64840..65505
                     /gene="ykgH"
                     /locus_tag="ACHKJF_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP0948040.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLFFLYINK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFAGQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAETEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(65751..66446)
                     /gene="ykgG"
                     /locus_tag="ACHKJF_000623"
     CDS             complement(65751..66446)
                     /gene="ykgG"
                     /locus_tag="ACHKJF_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0948041.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(66439..67866)
                     /gene="ykgF"
                     /locus_tag="ACHKJF_000624"
     CDS             complement(66439..67866)
                     /gene="ykgF"
                     /locus_tag="ACHKJF_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0948042.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(67877..68596)
                     /gene="ykgE"
                     /locus_tag="ACHKJF_000625"
     CDS             complement(67877..68596)
                     /gene="ykgE"
                     /locus_tag="ACHKJF_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0948043.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT        16563 a        18656 c        17157 g        16508 t
ORIGIN      
        1 taaaaacatt acccagtcga acctccgggt ctgaaagccc ttgcttttca gttattagca
       61 acatttaaca tttataaatt aacatctggc tgcgccagag attacgtctc tgagtgcatc
      121 gttgtgcttg tagtataata aataaaatgg agtgtcatta agtggataag gttttagatt
      181 cagccctcct ttcttcggca aataaaagaa agggtatcct tgctattggc gcacatcctg
      241 acgatataga attaggctgt ggcgcatcgc ttgctcgtct tgcgcaaaaa ggaatttata
      301 tcgcagcagt ggtgatgact accggcaact ctggtacaga tggaataatc gatcgccatg
      361 aagaatcgcg caacgcccta aagatattag ggtgccacca aactattcat cttaattttg
      421 ctgacacccg cgctcattta cagctcaatg atatgatttc cgccctggaa gacatcatta
      481 aaaatcaaat tccttctgat gttgaaatca tgcgggtata taccatgcat gatgccgacc
      541 gccatcagga tcatctcgct gtttatcaag cttcaatggt tgcctgccgc actattccac
      601 aaattctcgg ctacgaaacc ccaagtacct ggctttcatt tatgcctcag gtttttgaat
      661 ccgttaaaga agaatatttc acagtcaaac ttgctgcatt aaaaaaacat aaaagccagg
      721 aacgacgtga ttatatgcgt catgatcgcc tgcgtgcagt tgcacaattt cgcgggcaac
      781 aggtcaatag cgatctgggt gaaggctttg taattcataa aatgattctt tgagcaaatt
      841 gcatgaaaac ctggatattt atctgtatgt ccatagcaat gttgctatgg tttttaagta
      901 cgctaagacg taaacccagt caaaagaaag gctgtattga cgccattata cctgcgtata
      961 acgaaggccc gtgtctggcg cagtcactgg ataatctact gcgtaaccct tatttttgcc
     1021 gggtaatttg cgttaacgac ggctccacgg acaataccga agcggtcatg gcggaagtca
     1081 aacgcaaatg gggcgaccgc tttgttgccg tcacgcaaaa aaataccggt aaaggtggtg
     1141 cgctgatgaa tggcctcaat tatgccacct gcgaccaggt ttttttaagt gatgccgaca
     1201 cctatgttcc gcccgatcaa gacggaatgg gctatatgct ggcagaaatt gagcgcggtg
     1261 ctgatgccgt aggcggcatt ccctctactg cgttgaaagg cgcggggctg ttaccgcaca
     1321 tccgcgcgac cgtaaagttg ccgatgattg ttatgaagcg cacgctacag cagctcctgg
     1381 gcggcgcacc gtttattatc agcggtgcct gcgggatgtt ccgtactgat gtattgcgta
     1441 agttcggttt ctcggatcgt actaaagtcg aagaccttga tctcacctgg acattagtgg
     1501 caaacggcta ccgtattcgg caggcgaatc gctgcatcgt atacccacag gaatgcaaca
     1561 gtccgcgtga ggagtggcgt cgctggcggc gttggattgt gggatacgcg gtctgtatgc
     1621 gcctgcataa aagactttta tttagccgct tcggtatctt cagtatattt cctatgctgt
     1681 tggttgtgat ttatggcgtt gggatttatc tcactacctg gtttaatgaa ttcatcacca
     1741 ccgggccgca tggtgtggtg ttggcaatgt ttccgcttat ctggatcggc gtagtttgtg
     1801 ttattggtgc ttttagcgcc tggtttcatc gttgctggtt gttggtgcct ttagcgccgc
     1861 tttccgttgt gtatgtatta ttagcttatg ccatctggat tatttatgga cttattgcct
     1921 tttttactgg acgcgaacct cagcgcgaca aacccacccg ctattccgca ctggtggaag
     1981 cgtcaaccgc ttattcccaa ccttctgtca caggaactga aaaactatct gaagcttaat
     2041 gttaaagaga aaaatattca gattgcagac caggtaatta ttgatgaaac tgcaggtgaa
     2101 gttgttatcg gcgcgaatac ccgtatttgt catggtgccg ttattcaggg tccggtagtg
     2161 attggcgcaa actgcctgat aggtaattat gcgtttattc gtcctggcac aataatcagc
     2221 aatggtgtaa aaattggttt tgccaccgaa attaaaaatg cggttattga agcggaagca
     2281 acgattggtc cgcaatgttt tattgccgac tcggtagttg caaaccaggc atatttgggc
     2341 gcacaagtac gtaccagtaa tcatcgtctg gatgaacagc ctgtgtctgt tcgaactcca
     2401 gagggaatta tcgctaccgg atgcgataaa ttaggttgtt atatcgggca gcgttcacgc
     2461 cttggcgtac aagttataat tttgcctggg cgaattattt ccccgaacac gcaacttggc
     2521 ccgcgcgtga ttgtagaacg taatttacct gccggaactt attcactccg acaagaactt
     2581 atccgtacag gagattaaaa tgataaaacg gacgttatta gcggcggcca tttttagcgc
     2641 attgcccgct tatgccgggt taacttccat taccgcgggc tacgatttta ccgattattc
     2701 tggcgatcat ggcaaccgta atttagcgta tgctgaactg gtggcgaaag ttgaaaacgc
     2761 aacgctgctt tttaatcttt cccaggggcg tcgtgattat gaaactgaaa atttcaatgc
     2821 cactcgcggt cagggtgcgg tctggtataa atggaataac tggctgacaa cccgaacggg
     2881 tattgccttt gcggataata cgccggtctt tgcccgccag gattttcgtc aggatattaa
     2941 cctgaccctg ttgccaaaaa cgcttttcac gaccggttat cgctacacta aatattacga
     3001 tgatgtcgaa gtcgatgcct ggcaaggcgg cgtatcactc tatactggcc cggtgatcac
     3061 cagctaccgc tatacccatt atgactccag cgatgcaggt ggtagttata gcaatatgat
     3121 ttccgtgcgt ctgaatgacc cgcgcggcac tggttatacg caactatggc taagccgcgg
     3181 aacaggcgct tacacctatg actggacgcc agaaacacgc tacggcagca tgaagagcgt
     3241 cagtttgcaa cgtattcaac cgctgactga gcaacttaat ctcgggctga cggcaggtaa
     3301 agtgtggtac gacaccccaa ccgatgatta taacggtctg caacttgcag cccatctgac
     3361 ctggaaattc tgattccttc tgccgcccgc tatccggggc ggccttccct gccgattagc
     3421 cccccctttc ctctttgttt tccgaccaca ttcaccggat aaattttatt ctccagtgtt
     3481 atatactata ggggggtatg cattgacata tagcataccc cctatagtat attgcgtgca
     3541 gatgatgagg tgcgaaatgc ccagtactcc ggaagagaag aaaaaggtcc ttactcgagt
     3601 tcgtcgtatt cgagggcaga ttgatgctct ggaacggtcg ctggagggtg atgccgaatg
     3661 ccgtgccata ctccaacaga tcgctgccgt tcggggcgcg gctaatgggc tgatggcaga
     3721 agtgcttgaa agccatatcc gggaaacgtt tgaccgaaat gactgctaca gccgcgaagt
     3781 cagccaatcc gttgacgaca ctattgaact ggttcgagcc tatcttaaat agctgaatct
     3841 attaccatat tgaggaagag cgagagatga aatcacgtgc tgccgttgca tttgctcccg
     3901 gtaaaccgct ggaaatcgtt gaaattgacg ttgcaccacc gaaaaaaggt gaagtgctga
     3961 ttaaagtcac ccataccggc gtttgccata ccgacgcatt taccctctcc ggggatgacc
     4021 cggaaggtgt attcccggtg gttctcggtc acgaaggggc cggcgttgtg gttgaagtcg
     4081 gtgaaggcgt aaccagcgtc aaacctggcg accatgtgat cccgctttac accgcggagt
     4141 gcggcgagtg tgagttctgt cgttctggca aaactaacct ctgtgttgcg gttcgcgaaa
     4201 ctcagggtaa aggcctgatg ccagacggca ccacccgttt ttcttacaac gggcagccgc
     4261 tttatcacta tatggggtgc tctacattca gtgaatacac cgtggtcgcg gaagtgtctc
     4321 tggccaaaat taatccagaa gcaaaccatg aacacgtctg cctgctgggc tgtggcgtga
     4381 ccaccggtat tggcgcggta cacaacacag ctaaagtcca gccaggtgat tctgttgccg
     4441 tatttggtct tggcgcgatt ggtctggcag tggttcaggg cgcgcgtcag gcgaaagcgg
     4501 gacggattat cgctattgat acgaacccga agaaattcga tctggcgcgt cgcttcggtg
     4561 ctaccgactg cattaacccg aatgactacg acaaaccgat taaagatgtc ctgctggata
     4621 tcaacaaatg gggtatcgac catacctttg aatgcatcgg taatgtcaac gtgatgcgtg
     4681 cggcgctgga aagtgcgcac cgcggctggg gtcagtcggt gatcatcggg gtggcaggtt
     4741 ctggtcagga aatctccacc cgtccattcc agttggtcac tggtcgcgta tggaaaggtt
     4801 ccgcgtttgg cggcgtgaaa ggtcgttccc agttaccggg tatggttgaa gatgcgatga
     4861 aaggtgatat cgatctggaa ccgtttgtca cgcataccat gagccttgat gaaattaatg
     4921 acgccttcga cctgatgcat gaaggcaaat ccattcgaac cgtaattcgt tactgatttc
     4981 ccgcaggtat accccgtcca cttcagacgg ggttcttaat actctccctg ggcagccgtc
     5041 cgggggatta accgtgagat aatgactgat ggaactcatt gaaaaacatg ccagctttgg
     5101 cggctggcaa aatgtgtatc ggcattattc ccaatcactg aaatgtgaaa tgaatgtcgg
     5161 cgtctatctc ccaccaaaag ccgcgaatga aaaattgccg gtgctgtact ggctttcggg
     5221 cctgacctgc aacgagcaga atttcattac taaatcgggg atgcagcgtt acgcggctga
     5281 acacaacatt attgttgttg cgccggacac cagtccgcga ggcagtcatg tcgcagatgc
     5341 cgaccgttac gatctcgggc aaggtgccgg gttttacctg aacgcaacgc aagcgccgtg
     5401 gaatgaacat tacaaaatgt atgactatat ccgcaacgag ctgccggatt tagtgatgca
     5461 acattttccg gcaacgacca gaaagtctat ctctggtcat tctatgggcg ggctgggcgc
     5521 gctggtgctg gcgttacgta acccagatga atatgtcagc gtctcggcgt tttcgcccat
     5581 tgtctcccca tcgcaagtac cgtggggaca gcaagccttt gccgcatatc ttggtgaaaa
     5641 taaagatgcc tggttgaatt acgatccggt gagtcttatt tcacaaggtc aacgcgttgc
     5701 ggaaatcatg gttgatcagg ggttgagtga tgatttttac gcggaacagc tgcggactcc
     5761 aaatcttgaa aagatctgcc aggagatgaa tatcaagacg ttaatccgtt atcacgaggg
     5821 ttacgaccac agctattatt ttgtctccag ttttattggc gagcatattg cctaccacgc
     5881 caataaactg aatatgcgtt gataatagtg cacgactgcc ggatgcggcg tgaacgcctt
     5941 atccgcctac atttcgcccg caggccgtag gcatgataag acgcgcaaag cgtcgcatca
     6001 ggcatggcgc acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgccca
     6061 taaaccgtag gcctgataag acgcgcatag cgtcgcatca ggcatctgcg cacgacttat
     6121 ctatgtttac cacatcaaat catcaggcac tttgaagtcg gcatacgggt cgtcttcatc
     6181 ctgctcttcg gcactgagcg cgctgtgcaa cacaatactg ctagcatcac gctgagcaat
     6241 tttatcggca acgctggcgg gaataatggc gtattcacct tcgctattgt tatcgaccaa
     6301 caagcgggca atcgccagac gaccattaat caactgcgct tgtgtgagct tatcgacaaa
     6361 aatcttctta atcagattgc cgtcagtgaa gttaaaacca atatcgccat tggcaatggt
     6421 gattcggttc atttcaatga gctgcttcac ctgagcttta tattctttcg ccaacgccgc
     6481 ttgtttttgc tgttcgctaa gctgtttatc acgctcaagc tgtgcctttt tattttcttc
     6541 taccgccgcc cgagcttcac gcgcctgaac gcgcgatttt ttcgccgttc tctcgacctt
     6601 cgccgctttt ttgctggtga ctaatcctgc tttgagcaac tgctcttgta aggtaagttt
     6661 tgccatcttt gtttccaaac ccgctgaata atttttagcg agtatacctg taaacgccgt
     6721 gggtgtattg ggggcaagtc cctttcgccc ctgcttaata aggcgcgtca ggcatcggca
     6781 cacggctgca ttcttgatct ccgactcatc aaaataaaca ccgccaaccc agcaacaaga
     6841 atacccggtg cagaagcggc cattacgccg accgtgccag tgcctaatgc cagcattttc
     6901 ccggccagta acggaccgct catagccccc agacgcccta ccgccacggc tgttcccaca
     6961 cctgttgcgc ggatctgcga actgtaaaac aacggtgcca gggcatacaa aacgctttgt
     7021 ccacctgtcg caaacaaccc cgcgacaaat cccgccagca acataccgtt aaatgacgac
     7081 accgttccaa gcgccagcag cgaagctaac atgccgctat aaatcagtag cgacatggtt
     7141 actggacgca gcttatccat caatgcgccc aacattaacg tcccgcttgc cgcccccatt
     7201 tgcagggcaa acatcacccc tgccgcctgc gatggctgga atccttgctc caccaaaagt
     7261 agcggtagcc agttgatcaa catataaacc accagcagag tgaagaaata acacaaccac
     7321 agcagcagcg tcgcggttgc cgtttctggc gcaaataagg cacgcagtgg tggcgcagcc
     7381 tgtttttcgc cagcgaaaac cgctgactcc ggcagccagc gcattaatag cggcaccaga
     7441 atcaacggca ccacaccacc tacccaaaac accgtttgcc atgctaagtt tgcccccgcg
     7501 aaacccagtg tcgccgccag cgccgcgcca atgggaacac cgcaatacat caggctcact
     7561 gccgtcccac gaaaacgtgg acccgcggct tcagacgtca gggcgataag attcggcaac
     7621 gccgccccca gcccgacacc ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag
     7681 gcaatcgccg ttgccagtga gaacaaacca aacagcgcaa ctgagccaat caaaatacgc
     7741 ttacgaccat aacggtctgc cagcatcccg ccaaccaacg cgccgggtag caaaccgagt
     7801 attccggcgc taaatatcca gcccatttgc attttatcga gtgcgaaagc ctgggcgatg
     7861 ccacccgccg caatgccagc cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa
     7921 caaagcccga tggtcagcat caggcgggat gaagatgatg aaggggtacg agtcgacata
     7981 agatgaacct cattgcgagt gcaggtgaat cgcgactgat acattgccag ccgcgtaatc
     8041 agaactattt aatgactgcc tacgggcgaa atgtaggccg gataaggcgt tcacgccgca
     8101 tccggcagtc gtgctgggat gggcatacaa aatgcacgtt ctcggcagtc atcattattt
     8161 gttgttgcgc agatccagcg ccacgtcaac gatcatatcc tcctggccgc caaccatccg
     8221 gcgtttgccc agctcaacga gaatatccac cgcacttaag ccataacgcg ccgccgccgt
     8281 ttcacagtga cgcaggaagc tcgagtaaac accagcgtat cccagcgcca gcgtttcgcg
     8341 atcgactcgt accggtcgat cctgcaacgg acgcaccagg tcgtcggcgg catccattaa
     8401 cgcatagaga tcggtcccat gctgccagcc cagtttatcc gcggcggcaa taaacacttc
     8461 cagcggtgcg ttacctgcgc ccgcgcccat tcccgcgagg ctggcgtcga ttcggtcgca
     8521 gccctcttcc accgccgcga tagagttcgc cacgccaaga ctcaggttat ggtgagcgtg
     8581 catgccagtt tacgtttctg gtttcagctc tgctttcagg gcgcggaaac ggtcacggat
     8641 atcgctcatg ttcatcgcac cgccagaatc caccacataa atacaggtcg caccgtagcc
     8701 ttccatcagc tttgcctgct ttgcgagatt ctccggcgtg gtcatatggc tcatcatcag
     8761 aaaaccaacg gtgtccattc cgagctcgcg ggcatactga atatgctggg cggaaacatc
     8821 agcttcggta cagtgcgttg ccacacgaac cacccgcgcg ccagcctgcc aggcattttt
     8881 cagatcgtga atagtgccga ttcctggcag caacaacgtc gcgattttgg cgtgcttcac
     8941 cacatccgcc gctgcttcaa tccattcaag gtcgctatgt gcgccgaaac catagttaaa
     9001 gctggaaccc tgcaaaccgt cgccatgggc cacttcaatc gaatccacgc gggcatcgtc
     9061 cagtgctttg gcaatctggc gaacgttttc cagcgaatac tgatgacgaa tggcgtgcat
     9121 accgtcacgc aacgtgacgt ccgagatata aagtttttta ccgttcatgc cgcttctcct
     9181 gccttgcgcg ccagtgactg ggccattttt tccgctgtcg ccagcgcact ggaagtcata
     9241 atgtcgaggt tgcccgcata ggcaggcaga taatgcgctg cgccttcgac ttccagccag
     9301 accgctgttt tcagtccgga gaattgcccc acgcccggta aattgaccgg tttatcctgc
     9361 gggataactt caaactgcac gcgctgtttc aggcgataac ccggtacgta agcctgcacc
     9421 gcctcagcca tttcattgat tgaggcttcg atatcatctt gtgaagcttc gtcgctcaat
     9481 acatacaccg tgtcacgcat catcaacggt ggctctgctg ggttaagcac aataatcgcc
     9541 ttcccttttg ccgcgccgcc caccacttca atggctcggg aagtggtttc cgtaaattca
     9601 tcgatattgg cacgcgtgcc aggtccggca gatttactgg cgatagaagc gataatttcg
     9661 gcgtaatgaa cacgcgccac gcgtgaaact gccgccacca ttggaatggt ggcctggccg
     9721 ccgcaggtga ccatgttgac gttcagttga tcgacgttcg cctcgaggtt aaccaccggc
     9781 acgcagtaag ggccgatggc agcaggcgtc aggtcaatta agcgaatatc cggtttcgct
     9841 tcgcgtaaag cggcatcgtt tttcacatgc gcgcccgcgc tggtcgcatc aaatacaatg
     9901 tcgatatcag caaattcagg catgttcatc agcccgatca ccccttcatg ggtggtggcg
     9961 acgcccatac gtctggcacg cgccagaccg tcggactgag gatcaatgcc gaccatcacc
    10021 gccatctcca gatgctgacc gtgacgcaaa attttaatca tcagatcggt gccaatgttg
    10081 ccagaaccga taatggcgac tttacgctta ctcatgacag acttcctttt ggggctgcgc
    10141 ttgaaaatgt cgcagcaact gaacctatgc cttcaatatg ggcttcaaaa cgatcgcccg
    10201 cattcaccgc caccatcggg cctaatgccc cggtaagaat gatatctccg gcgcgcagcg
    10261 gttcacccag actggccatt ttgcgtgcca gccagacggc ggcattaagc ggatgtccca
    10321 ggcattcgct gccgcgcccg ctggaaacct cttcgttatt acgcgtcatc ttcatggcgc
    10381 agtttttcag gtctaacccc gccggacgtt gcgccggacc gccgatgaca tacaccccgc
    10441 aggaagcgtt atctgccacg gtatcgacaa actgaatcga ccagtcgcga atgcggctcc
    10501 ccaccacttc cagcgccgga agtacccatt caatggcgtt atacagttcg tcgaaggtga
    10561 tatcagttgc gggtaaatcg cggttcaaca ccagtgcgat ctccgcttca atgcggggtt
    10621 gcagaacacg ggaaaaagga atgatttcgt tatcgccata acacatgtcg gcaaataacg
    10681 tcccaaaatc cggttgatca acgcccagtt gttgttgcac tttcggatgc gtcaggccca
    10741 ctttacgccc taccacgcga cgcccctgca caacgtcata ttgcacattt atgtgctgaa
    10801 tggcgtaggc cgcttcagcg ttatcgatac caatcagatc gcgcagcggc gcaatcgctt
    10861 cgccctgctc tgcggcgcgg cgtaaatccg ccgccagttg ctcaagagta tgcttcgtca
    10921 tgaccattcc ttaagggcgt gcgaggaaat tcagcaccaa ttgattgaaa gcgtcggcat
    10981 gttcccactg cgcccagtga ccacagtcgc ggaagatatg cagttcagaa ccggcaatgc
    11041 cggacagcag acgcagaccc gcatccatcg gcacaaagcg gtcgttgcgc ccccagacaa
    11101 tcagggtttg cgctttgatt tccgccagac gtgggccaaa atccgggaac tgtttcggat
    11161 tagcttccag gctcttaacg aagttttcca ggtgatcgcg gcgcgacagc atattattca
    11221 ggcgcgcttc aaacagggcg tcggtcaaat cgctggtatc gaaaacgaag atatccatca
    11281 tcagcttcag gttttcgata gtcggctgac gataaagctg attcagtcgc ttaatacctt
    11341 cggttggcat cggcgtaaac aaactcatgc cgcccgtacc gccgcccatc agcaccagtt
    11401 tgccgacgcg ctccggccag ttaagggtga acgccacaga actatggccg cccatcgagt
    11461 tgcccagcag gtggattttg gcgatatcca gttgatccac cacgcttttc aggattcgtg
    11521 cattaagatc cgatcgtgaa ccactattaa cgatcgaatc gcttttgccc caacccggac
    11581 aatccagcag gattacccga tagcccgcct ctaccagcgg atcgatattg cggctgaagt
    11641 tcgcccagcc agtagcaccc gggccggaac catgcagcag gacaacggtt tcgtcgcctt
    11701 gtccgcagtc attaaaatgg atgcgcagcg ttttacccgc ttcttctaca ttcagaaaac
    11761 ggctggtggc ggcttcggtt tgtggctgat aactcatcat cttctcctgc atattcagtt
    11821 ctctgttctg gcgcttaacg agccaaatcc ggcaatccac tccgggattg ggcggtaata
    11881 acgcccttcg ctacgccagt tgccaaacgc agaaatagcg gcaaaagcgg cgacccaggt
    11941 tttgatttca tgtgtcgact ttccggcaat ggcggaaagc tcttcgttac tgacggcatc
    12001 cagttcctgt atgcgtccct gctccagcaa agtcatgaac tggttatccc aaatcgggtt
    12061 gagcggatgc agagttctct gatcctcaac aaacttctca gcggcgctaa tcacccgttg
    12121 ctgacgcaat tcgcgctcac tggcgggtaa atctttcccg ctccccaaca gacggtcgcg
    12181 catatgggca tcggctttcg ccagttcggg caccggcggc tgatgggaaa gcccaccgga
    12241 acccaggaac agcacgcgtt tattgagagt gctggtgaaa cgtccaatgg cttcacccaa
    12301 catgcgggta cgctggaaac cgggcagcgg cgtggcgaca ccgttgatga acacaggcag
    12361 aactggcacc ttatccagcc caccgagcag gaactccagc ggctgggcga acccgtggtc
    12421 cacctgcata cagtaagaaa cggcaagatc gatcccgctc ttcatgacag catgcgcaca
    12481 ggcctccgcc agctccacag gcacgggcag ctctcctgcc gcactgccga aatcaccaat
    12541 tgccgtcgct ccaacgccta aacagaacgg tggcatcacg tcatagaaaa agccgttgta
    12601 gtgatctggc gcaaacagca ccaccagttc aggggagaaa gccgcaatac gctcgcgggc
    12661 gctggcaatc acgccattga cctcatcgag tacctcttgc gccgggtcga cataccccac
    12721 caacggcgag tgggaaagac agtgaagata agcgtgcata tcaggctacc ttttcgacag
    12781 aaacgtcggc atcagggcgg gtcagcgtca tcaccgacgc cagtttattc agcgtattgc
    12841 ccagggtttg cggaatggcg gtggcggcaa caaagcgatc cgggcgcatc accaccagcg
    12901 aagcattgtg ttgcgcgaac cagctacgca gacgaccttg cgtatcgccc acgcgtagca
    12961 cgccgtcgtg gttatcctgt gcggtatgaa tttgcacttc cggcaccacc tgaatgaagc
    13021 gtgtgcccaa cgcgcgccac tgctggattt gctcgtcgct catcccccac agtggattgc
    13081 atccccagcc aattaccgcg aagttcgcgc caatcgcgtt atcgagcagc gtcacgtcgc
    13141 cgttttccag cgtgactttc ggctgaataa acatcttgcc gaccggagag tgcttcgctt
    13201 cgccctcacg taccagcgca ccgccgtaat attgcggcat cggcttgaag cgcatttcga
    13261 ggaagtagcg ttttactggc ggcagataat tcagtagcca ggaaacgccg tcacgtaacg
    13321 taccctgcca gcgtttcggc ggagccagca cgttgcccgc cgtcacggac aggtcaatca
    13381 tcgctttggc gtgatcgcga cgttcttgtt gataggtatc gagcagcgca tcgcgggctt
    13441 tcccctggat aaccaacgcc agtttccagg cgaggttaaa ggcgtcgcgc ataccactgt
    13501 tatagccctg cccctgccac accggcatga tgtgcgcggc atcgcccgcc agcagtacgc
    13561 gatcaatacg gaaacgttgc gccagtcgcg cgttatgggt gtagacacgc tggcgaatca
    13621 attcaacatt gtccggatta ggcagcactt tgcttaacag cttgcgcata ttttgcggct
    13681 cacgcagctg ctcttcggtt tctcccggca tcaccataaa ttcaaagcga cgtaccgcat
    13741 gaggcagcgc ggcagaaaca tacgggcgca ccggatcgca acacaaatag atatgcggcg
    13801 tacttaacgg atcgttggcg atatctacca caatccactg atttggcgca gttttacctt
    13861 caaacggcac attcagagtg cgacggacaa aacttgctcc accgtcacag gctaccagcc
    13921 actgggcttt gactatttcc cgctgccctt ctgccgtttt caggtgcaag gtcacttcgt
    13981 cattttgctg gctgaaggcc tccagctcgc gggaaaacaa gcagcgcaca ttaggaaaac
    14041 gcgacacccc ttccagcatc accgcatcga cctgcggctg aataaaggcg ttacggcgcg
    14101 gccagccaaa ttcatcggtc attggctgaa tatcagcaaa acagcggcct ttcggggtga
    14161 gaaaacgcat cgcgtgccac ggcgtagtgt gcggcagaac attctcgacc aggccaaccg
    14221 actgcatggt gcgcagcgcc tcgtcatcaa taccaatcgc acgcgggtag tcgatcaact
    14281 tatcgagttt ctccaccacc agcacgtcaa tgcccatctg accgagatag ttcgccatca
    14341 tcagcccgac cggaccggca cctgcgatcg ccacctgaac gctatggtta acagcaggct
    14401 ggatgtcagg gtgttgtatt gccatttcag tacctcacga ctcggacaaa atgccgttgc
    14461 gcgcacagta cggcgcaact tattttgtta aaaacatgta aatgattttt tattgtgcgc
    14521 tcagtatagg aagggtgttt tcggctacaa tcaaaacatg cccgaatgtg caccaggtgc
    14581 acaacgttgt tttaactata gaaatgtcaa ttaatatgca gaacaatgag cagacggaat
    14641 acaaaaccgt gcgcggctta acccgcggtc taatgttatt aaatatgtta aataaacttg
    14701 atggcggtgc cagcgtcggg ctgctggcgg aactcagcgg cctgcatcgc accactgtgc
    14761 ggcgactgct ggagacgctg caggaagagg gatatgtccg ccgtagcccc tccgatgata
    14821 gttttcgact gaccatcaaa gtgcggcaat taagcgaagg atttcgtgac gaacagtgga
    14881 tttctgcact ggcggcccca ctgctgggcg atctgttgcg cgaagtggta tggccgacag
    14941 atgtgtccac gctggatgtt gatgcaatgg tggtacgcga aaccactcac cgtttcagcc
    15001 gcttatcctt tcaccgggca atggtcgggc gacgtttgcc gcttctgaaa accgcctcgg
    15061 gcctgacctg gctggccttt tgcccggaac aagaccgcaa ggaattaatc gaaatgttag
    15121 cctcccgccc cggtgatgac tatcaactgg cacgggaacc gttaaagctg gaagccattc
    15181 tggcgcgcgc gcgcaaagag ggttacggac agaactaccg cggctgggat caggaggaga
    15241 agatcgcctc tatcgccgta ccgctgcgca gtgaacaacg ggtgattggc tgtctgaatc
    15301 tggtgtatat ggcgagcgca atgaccattg aacaggcagc ggaaaagcat cttccggcgc
    15361 tacaacgggt agcaaaacag atcgaagaag gggttgaatc gcaggctatt ctggtggccg
    15421 gacggcgaag cggcgtgcat ttacgttgac accatcgaat ggcgcaaaac ctttcgcggt
    15481 atggcatgat agcgtccgga agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg
    15541 ctatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac
    15601 caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg
    15661 aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc
    15721 gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct
    15781 cgcgctgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa
    15841 gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gctcattaac
    15901 tatccgctgg atgaccagga tgccactgct gtggaagcag cctgcgctaa tgttccggcg
    15961 ttatttcttg atgtctctga ccagactccc atcaacagta ttattttctc ccatgaagac
    16021 ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta
    16081 gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg cgggctggca taaatatctc
    16141 acacgcaatc aaattcagcc gatagcggaa cgggagggcg actggagtgc catgtccggt
    16201 tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc
    16261 aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt
    16321 gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg
    16381 ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg
    16441 caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa
    16501 agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
    16561 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
    16621 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
    16681 tatgttgtgt gaaattgtga gcggataaca atttcacaca ggaaacagct atgtccatga
    16741 ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttacac
    16801 aacttaatcg ccttgcggca catccccctt tcgccagctg gcgtaatagc gaagaagccc
    16861 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
    16921 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
    16981 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
    17041 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
    17101 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
    17161 ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg tcgctgggtc ggttacggtc
    17221 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc
    17281 gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt
    17341 ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca
    17401 gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg
    17461 aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg
    17521 gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg
    17581 gtagttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
    17641 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga
    17701 ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc
    17761 tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc
    17821 atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca
    17881 actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg
    17941 accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa
    18001 tgaatcgtct gactgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
    18061 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag
    18121 gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc
    18181 cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt
    18241 acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat
    18301 ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg
    18361 gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac
    18421 agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
    18481 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta
    18541 tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc
    18601 agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc
    18661 tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc
    18721 tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc
    18781 ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac
    18841 cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg
    18901 cggaaaacct cagtgtgacg ctcccctccg cgtcccatat catcccgcaa ctaaccacca
    18961 gcgaaacgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgtcagtcag
    19021 gccttctttc acagatgtgg attggcgatg aaaaacaact gctgacgccg ctgcgcgatc
    19081 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
    19141 ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt
    19201 tgcagtgctc ggcagataca cttgccgacg cggtgctgat tacgaccgct cacgcgtggc
    19261 agcatcaggg aaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
    19321 aaatggcgat taccgttgat gttgaagtag cgagcgatac accgcatccg gcacggattg
    19381 gcctgacctg ccagctggcg caggtcgcag agcgggtaaa ctggctcgga ttagggccgc
    19441 aagaaaacta tcccgaccgc cttactgcgg cctgttttga ccgctgggat ctgccattgt
    19501 cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg
    19561 aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca
    19621 gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat
    19681 ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag
    19741 tgtcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
    19801 aataataata accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt
    19861 actatttaaa aaacacaaac ttttggatgt tcggtttatt ctttttcttt tactttttta
    19921 tcatgggagc ctacttcccg tttttcccga tttggctaca tgacatcaac catatcagca
    19981 aaagtgatac gggtattatt tttgccgcta tttctctgtt ctcgctatta ttccaaccgc
    20041 tgtttggtct gctttctgac aaactcgggc tgcgcaaata cctgctgtgg attattaccg
    20101 gcatgttagt gatgtttgcg ccgttcttta tttttatctt cgggccactg ttacaataca
    20161 acattttagt aggatcgatt gttggtggta tttatctagg cttttgtttt aacgccggtg
    20221 cgccagcagt agaggcattt attgagaaag tcagccgtcg cagtaatttc gaatttggtc
    20281 gcgcgcggat gtttggctgt gttggctggg cgttgtgtgc ctcgattgtc ggcatcatgt
    20341 tcaccatcaa taatcagttt gttttctggc tgggttctgg ctgtgcactc atcctcgccg
    20401 ttttactctt tttcgccaaa acggatgcgc cctcttccgc cacggttgcc aatgcggtag
    20461 gtgccaacca ttcggcattt agccttaagc tggcgctgga actgttcaga cagccaaaac
    20521 tgtggttttt gtcactgtat gttattggcg tttcctgcac ctacgatgtt tttgaccaac
    20581 agtttgctaa tttctttact tcgttctttg ctaccggtga acagggtacg cgggtatttg
    20641 gctacgtaac gacaatgggc gaattactta acgcctcgat tatgttcttt gcgccactga
    20701 ttattaatcg catcggtggg aaaaatgccc tgctgctggc tggcactatt atgtctgtac
    20761 gtattattgg ctcatcgttc gccacctcag cgctggaagt ggttattctg aaaacgctgc
    20821 atatgtttga agtaccgttc ctgctggtgg gctgctttaa atatattacc agccagtttg
    20881 aagtgcgttt ttcagcgacg atttatctgg tctgtttctg cttctttaag caactggcga
    20941 tgatttttat gtctgtactg gcgggtaata tgtatgaaag catcggtttc cagggcgctt
    21001 atctggtgct gggtctggtg gcgctgggct tcaccttaat ttccgtgttc acgcttagcg
    21061 gccccggccc gctttctcta ctgcgtcgtc aggtgaatga agtcgcttaa gcaattaatg
    21121 tcggatgcgc cactgggcac atccgacaat tatttatagc tccatcatct ggcgaaacaa
    21181 gcgtcgcatc atacctcttt gacccacagc tgcggaaaac gtgctggtgc aaaacgcagg
    21241 gttatgatca tcagcccaac gacgcacagc gtatgaaatg cccagtccat caggtaattg
    21301 ccgctgatac tacgcagcaa accagaaaac cacggggcaa gcccggcgat gataaaaccg
    21361 atcccctgca taaacgccac cagcttgcca gcaatagccg gttgcgcaga gtgatcgagc
    21421 gccagcagca aacagagcgg aaacgcgccg cccagaccta acccacacac catcgcccac
    21481 aataccggca attgcatcgg cagccagata aagccgcaga accccaccag ttgtaacacc
    21541 agcgccagca ttaacagttt gcgccgatcc tgatggcgag ccatagcagg catcagcaaa
    21601 gctcctgcgg cttgcccaag cgtcatcaat gccagcaagg aaccgctgta ctgcgcgctg
    21661 gcaccaatct caatatagaa ggcgggtaac caggcaatca ggctggcgta accaccgtta
    21721 atcagaccaa agtaaacacc cagcgtccac gcgcgaggag tgaaaaccac gcgaaccgga
    21781 gtggttgttg tcttgtggga agaggcgacc tcgcgggtgc tttgccacca ccaggcaaag
    21841 agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt gataccaggt ttcgctatgt
    21901 tgaactaacc agggcgttat ggcggcacca agtccaccgc cgcccatcag agccgcggac
    21961 cacagcccca tcaccagtgg cgtgcgctgc tgaaaccgcc gtttaatcac cgaaggcatc
    22021 accgcctgaa tgatgccgat ccccacccca ccaagcagtg cgctgctaag cagcagcgca
    22081 ctttgggggt aaagctcacg catcaatgca ccgacggcaa tcagcaacag actgatggcg
    22141 acactgcgac gttcgctgac atgctgatga agccagcttc cggccagcgc cagcccgccc
    22201 atggtgacca ccggcagagc ggtcaacagg gcagccacgc taaagctcat tccgctcgcc
    22261 tggcgcaatt gcggtagcag tggcccgacg gaggtgagca gtggtcgcat attaagacca
    22321 atcagcacca gaaccagcag catcgtgaac gtcttttgat gattgcgcat ggctgtcaga
    22381 acggcttggt cggcagatat ttaccatcta aggtaatgac cgcacgttcg ccaccttccg
    22441 ggtccgccac tttcttaacg tcgagtttga agttaatcgc gctgataatg ccatcgccaa
    22501 acttctcatg aaccaacgct ttcagggttg tgccgtacac ctgcaacatt tcatagaaac
    22561 gatacatcgt tgggtcggtt ggaatacggt catcaatgca gccacgcagt ggaatcatct
    22621 gcaacagtag aatggcgtct tcgtcgagat ccagcttcgc cccgaccagg cgggcggcgt
    22681 cggcaggaag cgcttgctgc cccagcaaag ccgcggttac gaaggcttct gccagaccgg
    22741 taccgtcggc aatctcggca aatgagagat cttttttagc tttgctgagc aaaatggcat
    22801 cggcaagatc aagacgaata ttgcggttaa tttgtgactg aatcatggtg gatctcctga
    22861 tggtttaaaa ataaggttac gctgcggtcg gttggcgtag cggtatggca caaacgcgag
    22921 gattagcggc cagtggcaca aactggcggg ttgcgccgtc aaaagctgcg atgctgccgc
    22981 tttcaatgtc gtagacccag ccgtacaggg cgatccgccc ctcttcgagc gccaggcgca
    23041 ccgatggatg agtttgcaaa ttagccaact gagcaatgac gttttcacgt accatcgccg
    23101 cagcttttga cggtaaatcg gaatgcgggc gcgcctcatt aacgacgcgg gctgaatcgg
    23161 cataacgcag ccagtgggag acggcaggca tatggtccat gcactgacag ctggcaatgg
    23221 cggtcatcgc gccacagttg gaatgaccac aaatcacaat gtcagatacc cgaagcgcag
    23281 cgacggcata ctccaccgaa gcagaaacgc caccgggttc cggcccgtag gaagggacga
    23341 tattgcccgc gttgcgaata acgaacagat cgccaggctc acgttgcgtc accagctcag
    23401 ggaccagacg gctgtcggag caggagataa aaagtgtgcg cgggctttgc tgtgtcgcca
    23461 gctgtttaaa caaggcttcc cgcttcggaa atgcctcgcg ctggaattta aggaatccat
    23521 caataatctc tttcaccgtt aacctctgtc tgtctctgga atgagaggca gactactcgc
    23581 cgattgtcat aaggtaaaag tctcatttat gatgagttct attggattta cttataggtt
    23641 ggcgaatgct ctctcgacat atcaactatt ttcttgccgt ggctgaacat ggcagcttta
    23701 cccgtgccgc cagtgcgttg cacgtctccc agcctgcgct ttcccagcag attcgccagt
    23761 tagaggagag tttaggcgtg ccgctgtttg accgtagcgg gcgaacgatt cgtctcactg
    23821 atgcaggaga agtctggcga cagtacgcca gccgggcgtt acaggaactg ggggcgggta
    23881 aacgggcgat tcatgatgtt gccgatctga cgcgaggatc gctgcgtatc gccgtcactc
    23941 ccacctttac aagctacttt atcggccctt taatggcgga tttctatgcg cgctaccccg
    24001 gcatcacgct ccagctacag gaaatgtcgc aggagaaaat cgaagatctg ctttgtcgcg
    24061 acgagctgga cgttgggatt gccttcgcgc ctgtgcattc gccggagctg gaggcaattc
    24121 ctttactgac agaaagttta gcgttggtgg tggcgaaaca tcatccgctg gctgcctgtg
    24181 aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt cctgctcagc gcggaatttg
    24241 ccaccagaga gcaaattgac cactactgcg agaaagcggg gctacatcca caggtggtca
    24301 ttgaggcgaa ctcaattagc gcggttctgg agctgattcg ccgcacttcc ctttccacat
    24361 tgttaccagc agcgattgcc acacaacatg acgggcttaa agcgatttct cttgccccgc
    24421 cactactgga gagaacggcg gttttgttgc ggcggaaaaa tagctggcag acagccgccg
    24481 cgaaggcatt tttgcacatg gcgttggagg aatgtgcgga tgttggcgag aatgaatcac
    24541 ggtagccact gacacaggtc agtggctctt caacacagag gaaattaccg tttgtaatcg
    24601 atggcttctg gctgctccag atatacggtg gtttgtgccg gttgtgtact ggcaatcacc
    24661 ttgccgccac gtaccgaata acgtaccgga acctgacggc gcagcgcatc aaatccattt
    24721 tcagccggca ggataatcag gtttgcgctg tttccggcgg caatgccgta atcctgcaaa
    24781 ttcaatgttc tggcactgtg gtgggtgatt aaattcaggc catcattaat ctgcccgtag
    24841 cccatcaact ggcaaacatg cagccccata tgcagcactt gcagcatatt cgccgttccc
    24901 agcggatacc acggatcgaa gacatcatcg tgaccaaagc agacgttaat gccggactcc
    24961 agcatctctt taacgcgcgt gatgccgcga cgttttggat acgtatcgaa acgtccttgc
    25021 agatgaatat tgaccagcgg gttggcgaca aagttaatac cggacatttt cagcaagcgg
    25081 aacaggcgtg aggtatacgc cccgttatag gagtgcattg ccgtggtgtg gctggcggtg
    25141 actcgcgcgc ccatgccttc acggtgcgcc agggcagcaa cggtttcgac aaagcgcgac
    25201 tgctcgtcat cgatctcatc acagtgaacg tcgatgagac ggtcgtattt ttgcgccagg
    25261 gcgaaggttt tatgcagcga ctccacgccg tattcacggg taaattcaaa atgcggaatc
    25321 gccccaacaa catctgcccc taagcgtaac gcctcttcca gcaacgcttc accgttggga
    25381 tacgacaaaa tcccttcctg agggaaggcg acgatttgca gatcaatcca cggcgcgact
    25441 tcctgcttca cttccagcat tgctttcagc gcggtcaggg ttgcatccga aacatcgaca
    25501 tgggtacgca catgctgaat gccgttggca atctgccatt tcagcgtttg ccatgcgcgt
    25561 tgtttcacat cgtcatgggt taataacgct ttgcgctcgg cccagcgttc aatgccttca
    25621 aacagcgtgc cggactgatt ccagttcggt tgtccggcgg tttgcgtggt gtccaggtga
    25681 atatgcggct ccacaaacgg cggtataact aaaccttgtt cggcatccag gctgttttca
    25741 gttatgggca tcacgccgga ttgcgcatca atggcgctga tttttccgtc ctgcagatga
    25801 atctgccaca gcccctcttt gcctggtaac cgggcgttaa taattgtttg taaagcgtta
    25861 ttcgacactg ttaacctcca catgcgtcat tgctgctgtc gttttacgat tcaaaatcgg
    25921 gttaaggatc agatagctca gcgcgccacc taataccgcg ttgaccggaa caattcccgg
    25981 taaccagtgg cctgcggcaa tccccagcgc gaccgccaga atcgccaccc aattgacact
    26041 catcatacgc gtggtcgcaa agtgctcata gcggcgacgg ttcatcagat agtcggcgat
    26101 gatcacgcca cctaccggag gaatagctgc cgaaaggaag gtcaaccagc cgacaaaatt
    26161 gttatacagc cataatgcgc agaccgtacc gataataccg ttgattaccg aaagggtttt
    26221 gctcgacata ccggtaatgt tggcgaaacc taaacccgac gcatagagtg cgttatcgtt
    26281 ggtggtccag atattcagcc ccagcaccac aatcgcaggc agcagcaggc cctgagcaat
    26341 catcacatca gagatatccg ccatgcccag tgccgcagcg cccgctgcac cgaaaataaa
    26401 catcaacgag ttgccgagga aaaaggccac catcgccacc agcaccgcca gtttggcatt
    26461 gcgaccaaac cggacaaagt cagcggtgag cgtacccgca ctgataaatg accccacaac
    26521 cagcgccagc gcgacattga aatctaacgg ttgtgcggga acgaccgctt ttaatgcgtc
    26581 caggccgccc atgccgttaa cagccagcca cacggaataa ccgcccaggc aggcgatagc
    26641 cggaaccgca atcaccgaaa gaaccgtcag cgccgaaata ccaaaaaaga cggtgacggt
    26701 catcagtaaa ccggaaacgg caatcagcaa attaatatcc agcccggttg ccttacccac
    26761 cggaatggca aacatcgcca caccgacgcc aaaccagcca acctgagttc cgcccagtag
    26821 cagtgaaggc agccatgagc ctttaacacc aaacgagaag cgagcaagaa gatgagtggt
    26881 caggccggtt tttgcgccaa tgtaaccgag aaatgaagtg taaataccga ggagaagatt
    26941 accgatgaga actgcgagga agaaatcatg atagctaaga ccggttccga gagtaccgcc
    27001 ggtccacata ctggcggaaa agaaggttaa tcccagcatg acgaacgtca atgccaatac
    27061 ccctttccgc gccgactgcg ggactggccc ctggctaaag ttgttatctt gcgacacgaa
    27121 attccccctt tttgctgttt gaaacccaaa aaatccgccg cattctattc atctgaagat
    27181 aaaaagcaat cgttttcgtg gggaaatata ttttttatat gaatgagtgc aaaaaggtgg
    27241 gggaaaaatt atcggtgtaa aaacagtggg taggcataat aagacgcggt aagcgtcgca
    27301 tcaggcatcg gcgcatggtg tcggatgcgc cacgcgacgc atccgacaat accttactct
    27361 tccatcgcct ggcggatctg atccaacgac gcaggatcat caatggtcgt cagatcccca
    27421 ggatcgcgtc cttcgcaaat cgcctggatc gtgcggcgca gcatttttcc ggatcgcgtt
    27481 tttggcaatt gcgagacaaa ccagacgtgc gccgggcggc caaagttgcc aatctggctg
    27541 tccaccagcg ccataatcgc cttctcttgc gagtgcgcca catcacgatc ttccagactg
    27601 tcgctctctt tcggaatgac aaacgccacc gccacctgcc ctttcagcgc atctttcacc
    27661 ccaaccaccg ccacttcggc aacgcccgga tggctggaga tactctcttc aatctcgcgc
    27721 gtccccagcc gatgcccggc aacgttaatt acatcgtcag tgcgcccgag aataaagtga
    27781 taaccgtcag cgtcacggat gccccagtca aaggtggcgt acaccgggcg ggaaaacagc
    27841 gaccagtaag tcttcacaaa gcggccgtcg tcgccccaga tggtctgaat acaccccggc
    27901 ggcagcggcc cttccaccac cagcatccct ttctcgttga cgccacacgg ttcgccggtg
    27961 acttcattaa gcaactgcac gttatagcca tacatcggca cgccggggct tcccagacgc
    28021 gtcggcctgt cgtccagacc gcgagcaatc gccataatcg gccagccgga ttcggtctgc
    28081 cagtagttgt cgatgaccga cacatccagc gtattgctca cccaactggc ggtcggctcg
    28141 tccagcggtt ctccagccag atagagcact tccagcgacg agagatcgtg tttacgaatt
    28201 tcagcggtag ggaatttttt cagcacgcga atagcggtcg gcgctgagaa catccggcta
    28261 acctgatatt tctcgacgat tttccaccac acgccgcagt ccgcccaggt cggcaatcct
    28321 tcgtaaacga tagtcgccat ccccgccagc agcggcgcgt aaacgatata cgaatgcccc
    28381 accacccagc cgatatccga tgcgcaaaag aacacgccgc ccgctttgcc gccaaaaatg
    28441 gtgtccatcg aggtcgccag cgccaccgca tatccgccga cgtcacgctg cacgccttta
    28501 ggtttgccgg tcgtgccgga agtgtagaga atgcaggagg tttcgttgga ttccagccac
    28561 gccactggta cccgcgcgcc gatgtgttga tagcgcaacg acgcgaaatc gacatcccgc
    28621 ccgctgacgc gcgccatttt cgccagtccg cgatccacca gcaaaacatg gcgtggctgg
    28681 tgctgcgcct gacttatcgc atcgtcgagc aattttttat agggaatgat tttgccaccg
    28741 cgcgctccgg catcagccga aacaatcagc accggtttag catcatcaat tcgcgccgcc
    28801 acgctgtgcg aggcaaatcc accaaacacc accgagtgaa tagcgccaat gcgcgcgcag
    28861 gccagcagag taatatgcgc ttcggcaatc atcggcatat acaccagcac ccgatcgcca
    28921 cgctgcacgc ccaatgaacg caacattgag gccaccgcgt ttacttcgtc atgaagctga
    28981 cgaaaggtaa aggtgcgctc ttcttctgtt tccgaagaga cggcaatcag cgccagcgcc
    29041 tctggttgtt tctccagcca gcggtcgata gcgttgtggc acaagttggt tcggccttca
    29101 caaaaccaac gggcaaacgg cggattgctg tgatcgagcg tttgcgtaaa gggcgtctgc
    29161 cagtcaatac gccgggcctg ctcggcccag aactgctccg gttcgttaat cgaacgctga
    29221 taaaattcgc taaaagacat aatgctctcc tgttgaactt acgccttacc gctgtttgct
    29281 taaatgacgt acaggtcgag atactcattg accggcatct gttccaggcg agttctgtcg
    29341 agagaaacct ccagaatgcg ctgctgttgg cgagtcggga actggcgcgc gagattgatt
    29401 ttgaatttat cgaccagttt cggaatacca tcctgacggc ggcgagcatg accaatgggg
    29461 tactccacca ccacttcttc aaatcgtgtg ccgtcggtga actcaagggt aatggcattg
    29521 gcgatggcgc gtttttccgg gtcgtggtag tcagcggtaa atgccggatc ttcaaagcaa
    29581 ttgatcttct cgcgcagggc gtcgatacgt ttatcttgcg ccacgttgtc ctcgtaatct
    29641 gccgccgtta agcgcccgaa cagcagcggg atcgccacca tgtactgaat gcagtgatcg
    29701 cggtctgccg ggttattgag cggtcctttt ttgtcgatga tgcgaataca ggcttcgtgg
    29761 gtgcgaatgg tcactttttc gatatccgcc gccgttttgc ctgctgcctg catctgttca
    29821 tagagcgtca tcgctgcttc aactgccgtc tgggagtgga actccgccgg gaaggagatt
    29881 ttgaacagca cattttccat aacgtaggaa ccgtacgggc gctggaagcg gaacgattca
    29941 cctttaaagg agacgtcgta gaagccccac accggcgcag tcagggctga cgggtaaccc
    30001 atttcgcccg ttttcgccat cagtgccaga cgtaccgcgc gggaagtggc atcgcccgcc
    30061 gcccaggatt tacgcgtgcc ggtgttcggc gcatggcgat aggtgcgcag cgactgaccg
    30121 tccacccacg ccagcgaaac ggcgttgaga atttcctcgc gggtcaggcc gagcatttcg
    30181 gcgaccacgg cggtggaagc cactttcact aacagaacgt ggtcgaggcc gacgcggtta
    30241 aaggagtttt ccagcgcgat gcagccctga atttcatggg ctttgatcat tgcggtcagc
    30301 acctgtttca tggtcaacgg cgctttgccg ctggcgaccg cgttgcgcga aagccagtcc
    30361 gccgttgcca gaatgccgcc gaggttgtcg gaaggatggc cccactccgc cgccagccag
    30421 gtatcgttga aatcgagcca gcggatcatc gcgccgatgt taaatgccgc ctggacgggg
    30481 tcgagctgga actgagttcc ggggacgcgc acgccgttgg gtacgacggt gccgggaaca
    30541 attggcccca gcagtttttt acaggccggg tattcgagag cttccagacc gcagccgagc
    30601 gtgtcgagca ggcagtaatg tgcggtgtcg taggccactt tagagctgat ttcgtagttc
    30661 atgacgtaat cgacgatatc aacgatttca cgatcaaatt ccgggcggat gttgttgatt
    30721 tgagctgaca ttgggtacgt ttccttattg ttattcgtag aggtttactg gcgcttatcc
    30781 agcgcgacaa acgggcggtc ttccggtcca acataattgg cggaaggacg gataattttg
    30841 ttgtcctgac gttgttcgat aatgtgcgcc gcccagccgg tgacgcgggc gataacaaac
    30901 agtggtgtaa acatctcggt gggaacgccc atcatgttgt aggaaacagc agagaaccag
    30961 tctagattgg ggaacatctt tttgctctcc cacatcaccg tttccaggcg atcggcgatg
    31021 ttgtacatct tcagcgagcc gccttcctgc gagagctgct tcgccacacg tttaatcacc
    31081 tggtggcgcg ggtcagcgat ggtgtaaacc ggatgaccaa aaccaatgac cacttctttg
    31141 ttttccacgc gcttgcggat atctgcttcg gcttcgtccg gcgtttcgta gcgttgctgg
    31201 atctccagcg acacttcatt cgccccgccg tgttttggcc cgcgcagtgc gccaatcgcg
    31261 ccaataatcg cggaatacat atcagagccg gtgcccgcaa tcacccgact ggtaaaggtg
    31321 gaggcgttaa actcgtgttc ggcgtacagc accagcgaga tatgcatcgc cttttcccag
    31381 ctttgcgatg gcttttcgcc gtgcagcaga ttcaggaagt gaccgccgat ggagtcgtca
    31441 tcggtttccg gttggatgcg ttcgccgttg tggctgtagt gataccaata aaggagaatc
    31501 gagctaagcg acgccagcag tttgtcggca atatcccgcg cgccagagac ggtatgcccc
    31561 tctttttctg gcagcgtgca gccgagcgcg gaaacaccgg tgcgcataac atccatcggg
    31621 tgtgacgccg ccggtaaggc ttccagcacg gtacgcacgt tagccggtaa accacgcagg
    31681 gctttcagtt tcgttttgta agcggcgagt tcgtcacggg tcggcagttt gccgtggatc
    31741 agcagatgag ccacttcttc aaattcgcaa tgttccgcca gatcaagaat atcgtagcca
    31801 cggtaatgca ggtcattgcc acttttaccc acggtgcaga gcgccgtatt gcccgccgga
    31861 acgccggaaa gtgccaccga ttttttcggt ttaatgacat gggtactgtt ttgcaggatc
    31921 gttgtgtcgc tcatattgtc ctcgtcattt gtagggtcga gatatttttc gttgtgtttt
    31981 atttcacctg gctacgggca aacaggtcgt cgagcttctc ttcgtactgg tagtagttga
    32041 tgctttcgta cagttcgttg cgggtctgca tggtgtcgat gacgcttttc tgcgtgcctt
    32101 cctggcgcag gacgttgtag acatgttcag cggcgcggtt catggcgcgg aacgctgaaa
    32161 gcgggtacag cgccattgcg acatgggcgc tgcgtaattc gtcggtggta aacagcggcg
    32221 tggcaccaaa ttcggtaatg ttggcgagga ttggcacctg aaccgcatcg gcaaactggc
    32281 ggtacatggc gagttcggta atcgcctccg ggaacaacat ctcggcaccc gcttcaacat
    32341 aggcctgtgc acgctcgatc gccgcatcca gcccctctac cgccagcgca tcggtgcgcg
    32401 ccatgatcac aaaatcagga tcggttttcg catccaccgc cgcgcggatc cgatccacca
    32461 tctcctcttt cgagacgatc gctttgttcg ggcgatgacc gcagcgtttc gcaccaacct
    32521 gatcttcaat atgcaatcct gccgcaccgg ctttaatcat cgatttcacc gtacgcgcca
    32581 cgttaaaggc cgaagaacca aaaccgatat ccgcatccac cagcagcggc agcgaacaaa
    32641 cgtcggtgat acggcgaatg tcggtcagca catcatcaag ggtagaaata ccgagatcgg
    32701 gcagccccag cgaacctgcc gccacgccac cgccggagag ataaattgcc tgatatccgg
    32761 cacgctgcgc caacagcgca tgattagcgt tgatggtgcc aacaatctgc aatggatttt
    32821 ctttagacag tgcagcgcga aacgctttac ctggagagtg tagagacata gcccatcctt
    32881 tgttatcaac ttgttacttg cgttgataaa gacaaagcaa ggggtgtgcc acaattaact
    32941 gaataaaatt aaatgtttaa tttcaatttg ttataaataa agtcatgaac taatccgcaa
    33001 atatgcgttt cagttaacgt ttcaggcaat gtttcaccgc gtttcattgc aacaattatg
    33061 aaacaagact aaacccaata ttcggtttct taactttgcg gtgcgctatg gcacatccac
    33121 cacggcttaa tgacgacaaa ccggttatct ggacggtatc tgtaacgcgc ctgttcgagc
    33181 tgtttcgcga tatcagcctc gagtttgatc acctggcgaa cattacccct atccagcttg
    33241 gctttgaaaa agcagtgacc tatatccgca agaaactggc aaacgaacgc tgcgacgccg
    33301 tcatcgccgc tggctctaac ggcgcgtacc tgaaaagccg cctgtcagtg ccagttattt
    33361 tgattaaacc gagcggctac gatgtgttac aggcactggc aaaagccgga aagctcacct
    33421 cttctatcgg cgttgtcact tatcaggaaa ctattccggc actggtggcg tttcaaaaaa
    33481 cctttaattt gcgcctcgat caacgtagct acattaccga agaagacgcc cgcgggcaga
    33541 ttaacgagct caaagctaac ggcaccgaag cggtggtcgg tgcggggctg attaccgatc
    33601 tggcagaaga agccggaatg accggaattt ttatctattc cgccgtcacc gtgcgccagg
    33661 cgttcagcga tgcgctggat atgacgcgca tgtcgttacg ccataacact cacgatgcca
    33721 cccgcaacgc cctgagaact cgttacgtgc tgggcgatat gctcggtcaa tcaccacaga
    33781 tggaacaagt acggcaaact attttgctgt atgcccgctc cagtgcggcg gtgttgattg
    33841 aaggggaaac ggggacaggc aaagagctgg cggcccaggc gattcatcgg gaatattttg
    33901 cccgccacga tgcgcgacag ggcaaaaagt cgcatccgtt tgttgccgtc aactgcgggg
    33961 cgattgccga atcgctgctg gaagcggagc tgtttggcta tgaggaaggg gcgtttaccg
    34021 gctcgcgacg cggaggtcgc gccgggctgt tcgaaattgc ccacggcggt acgctgtttc
    34081 tggatgagat tggcgaaatg ccgctacctt tgcagactcg cctgttacgg gtgctggaag
    34141 aaaaagaggt tactcgcgtc ggcgggcatc agcctgttcc ggtggatgtg cgggtcatta
    34201 gcgccactca ctgcaatctg gaagaagata tgcggcaagg gcagtttcgc cgtgatctgt
    34261 tttatcggct gagtattttg cgtctgcaat tgccaccact gcgcgagcgg gtggcggata
    34321 ttcttccgct ggcggaaagc tttttgaaag tgtctctggc ggcgctctcc gccccgtttt
    34381 ctgccgcatt acgccaggga ttacaggcaa gcgaaaccgt gctggtgcac tacgactggc
    34441 caggcaatat tcgtgaactg cgcaatatga tggaacgact ggcgctgttt ttaagtgtgg
    34501 aaccgacgcc ggatttaacg ccgcagttta cgcaactgct actgccggag ctggcgcgcg
    34561 agtcggcgaa aattcccgct ccacgcttac tgacaccaca acaggcactg gagaaattta
    34621 aaggcgataa aacagcagcg gcgaattatt taggcataag ccggacgacg ttctggcggc
    34681 ggctgaaaaa ctgaactgcc gaatgcagcg cgaacgcttt atccggcata aatttcaata
    34741 gatgatgatc accgataaat ttacgcatcg gggatgttta cataggtttc agaattactt
    34801 cttcttataa atatttgccg tgccgtggat cttattgtca gtttgaccag aggtcagtac
    34861 caacacatca gccccttttt catccgcttt tttgatcaaa tcctctttcg catctgcggt
    34921 cgacatttca ttactggttg aaatatcacc tattttttca tactgcgatt caactttttc
    34981 aaattccgct ttggtcatca attcagcggc atagacattg gtaacggcta acgctaacgc
    35041 accgactaac attttagaga ttattttcat attacggctc cagagttgtt atcacactat
    35101 cacccaccca cattattggg ttgattaata attagttcag tcagggcata aacgccagta
    35161 accggaaata agacactttg tgatctctct cgcacccgat tatttttggt agaaatatga
    35221 tgaagttact tcacttattt atggatgaaa tcactatgga tcctttgcac gccgtttacc
    35281 tgaccgtagg actgttcgtg attacttttt ttaatccggg agccaatctc tttgtggtag
    35341 tacaaaccag cctggcttcc ggtcgacgcg caggggtgct gaccggactg ggcgtggcgc
    35401 tgggcgatgc attttattcc gggttgggtt tgtttggtct tgcaacgcta attacgcagt
    35461 gtgaggagat tttttcgctt atcagaatcg tcggcggcgc ttatctctta tggtttgcgt
    35521 ggtgcagcat gcgccgccag tcaacaccgc aaatgagcac actacaacaa ccgattagcg
    35581 cctcctggta tgtctttttt cgccgcggat taattaccga tctctctaac ccgcaaaccg
    35641 ttttattttt tatcagtatt ttctcagtaa cattaaatgc cgaaacacca acatgggcac
    35701 gtttaatggc ctgggcgggg attgtgctcg catcagttat ctggcgagtc tttcttagtc
    35761 aggcgttttc tttgcccgct gtgcgtcgtg cttatgggcg tatgcaacgc gttgccagtc
    35821 gggttattgg tgcaattatt ggtgtattcg cgctacgcct gatttacgaa ggggtgacgc
    35881 agcggtgagc agataattta ctctctacgt aatcaacctg atttgccgaa tcccggccat
    35941 cggcgatgat agcccccgta atgcgtggtc cttatttccg ataagtttac ttgagtcttt
    36001 agcttcccat ttcagcccac tctcccctgt gtgggctttt ttcattggtc tgccccgtcc
    36061 tgaatagcca aacaatggca atgttccact ttgcagggat gataataagg ggatctgatt
    36121 cagaagagtt ttgaaaagtt ggaccgccat cgaggcctcg aaccccgtac catacaacaa
    36181 tcctggttgt cgtctgctct tcctgatgag ctaatggcgg tttttgatat gagccttaca
    36241 gcatattttc tgtcaggctt gagtgcgatg ctaaggtaca gaaaagaccc cacacaaaca
    36301 tgatggaatt atgatttttt tcatatgaat gtcaacccac acattcacaa aacatctgca
    36361 tgagatcatt ttttatacac cgcatggctg cgaaaccact actggtgctt ttttaagcga
    36421 agtgtcgccg atcacaacaa aatatcggcg aaaaaagaga tggaatgcca gttggcatac
    36481 agatatacag atgaaatgat tacctgcatt gcgattcact ctcaggtgga ataacaactt
    36541 gcaggtggaa ccctttctga actaaagagt gtagcggatt ttaacgtgct tatttacagg
    36601 ataatatctg tcgcctaatt cctggactct attagatatc cattgattta atcgcgcatc
    36661 atctctgtat gtttttacgt ccagaacaga gagcaataac atcaccagtg attcaggaaa
    36721 aacagggtag atatttttta agtgtaataa taatccatgc gcaagcgcct gcttaatggg
    36781 cgcgtctgtc tccggaatag acgacatttc gcgaagccat ttttccacat gcatacactc
    36841 tttcccttta gggaaaattc tcagcaaagg catatggtgt tcaagaagaa tacatttgca
    36901 atctgttcgc actgaatcga taaactgcac taatttctta agcccggtaa agcgtccagc
    36961 ctgcacgttt gctttaatat caagaaacgc atcatcctgg tcatataccc aaactgtccc
    37021 tttttgattt tcaagtatgc gaaaaacaaa cttaaccggg tgatcagggt aatccatcca
    37081 gtcataaaat ccagggaaat cattaaactg cacatttatg gtgagtgcgt tatcgtctga
    37141 ccactcacag tctcgtacgg cagccatgag atgaatgagt ttgtcgtcat cgactaaatc
    37201 atcacgacaa tcataaggat aatcataata ttgtttaatc aaaaccttga atatatcgac
    37261 acaggaaacc agggagcttt tgatatcctc tggtaactga ttgtttttaa ccgtattaag
    37321 tatgctttgt tgcgtatatg gcatcaaatt attgtgcgga gcttttaatg atatcatcgc
    37381 gatctctctt aaataaaaat aaacgtcgca gataataaag ctctgattaa accaggcact
    37441 atcagaaatc gctcataact aacgtgtgaa gtattgtgta ctggagggag ttaatttaat
    37501 aatttgaatt tacgttatag gttttactgc tactttgata ttatttcgct ctccttaact
    37561 aaaaagttaa gtggagagcg aagttaatat gttaaaccac agcgtattta ttaatttttt
    37621 cagtctgtta gtgtgcgatt atcgataaca aaacgatatt tcacatcacc gcgcagcatt
    37681 cgctcatagg cttcattaat ttgatcggcc cgaatcatct ctatatcagc cacaatgcca
    37741 tgttcggcgc aaaaatcgag catctcctga gtttctggaa tgccgccaat catagaaccg
    37801 gctatcgcac ggcgtttcat gatcaggttg aaaacttccg gcgatttatg cggtgtcgca
    37861 ggcgcaccaa ccagcgtcat ggtgccatca cgcttcagca aggtggtaaa atcgtcgaga
    37921 ttatgtggcg cagctactgt attcaaaatg aaatcgaaac tcttcagatg agccgccatc
    37981 tcatcggcat tgcgtgagtt aacaacttca tcggccccca gggcttttgc cgcttcgcgt
    38041 tttgcctcag aagtggtaaa tgccaccaca tgtgccccca tcgcgtgggc cagcttaatc
    38101 cccatatgtc ccagaccgcc gatgccgacc acgcccactt ttttacccgg cccggcctgc
    38161 cagtgacgta gcggcgaata cgtggtgatc cctgcacaca acaaaggagc caccgccgcc
    38221 agctgctctt gcgggtgacg aatacgcaga acatatcgct catgaacgac gatctgttgt
    38281 gagtagccgc ccagagtatg gcccggttcg tccggcgtcg gcgagttata ggtgccggtc
    38341 atgtgatcac agtagttttc caacccgtct tcacactctt cgcaatgttt acaactgtcg
    38401 acaatgcagc cgacaccgac cagatcgccc ggcgcatatt tttctacctg atcaccaacg
    38461 gctaccacac gccccacaat ttcatgaccc ggcacgcagg ggtaaaccgt ccccgcccac
    38521 tcggaacgga cctggtggag atcggaatgg caaacgccac agtaagcgat ttcgattttg
    38581 acatcattcg gtcccggttc acgccgggtg atatccatcg gttcaagtgg ttgtttagcg
    38641 gaatatgcac caacagcttt gatcttcatt gtgtttactc ctgattagct atgtgtattt
    38701 ggcgacgcct gatatgtcat cgcggcaaaa cgcgtccatt gaatatagcc aatatttaaa
    38761 ccacccgatc cagccccaac atgccgcgag cctgaatgaa acagagcagc aacgttcccc
    38821 ataaggccat cgtcaggtag gggcttatac cgagaatatt gaagccgctt tcaataattt
    38881 gcagaagaaa cagcgccagc accataccaa ttatccgccc gctgccgccg tccggattaa
    38941 ccccgccgag tactgccgcg aggattgaca ccagcaagta cgattcgcca taagaggcct
    39001 tagccgagtt gagtttcgac atcatcaaaa atgccgctac cgcacaaagc aaggcggaaa
    39061 tgacataaac ccaaatcagc acccgacgag tgttaatgcc ggagtaaagt gttgctcgtt
    39121 cgttagaccc aatcaggtaa atggctttac cgagcggcgt tttctccagc aatatccata
    39181 gccccagtgc cactgcggca aacaaccaca tcggtagcgg aataccgaac cattgcgcat
    39241 gattcaacca cagcacccat gagggataat tggcaatggc gctaccacct gtcaccagaa
    39301 tatttatccc tttgagcaac gtcatcatcc caagcgttgc aagaattggc gacactcgaa
    39361 taccagcaat caatacgccg ttacataaac cgattatcac cgccgcgcca gcgcccgcca
    39421 gtaacgtagc gacaaccgta gcaatacccg gtggatactg agtcgccacc cacgccatca
    39481 ccagtgaaca ggcgttagca gtggctataa ttgacaagtt aatgccaccg caaagcatgg
    39541 tcaccgccat agccaacgcc agaatgccca gcacgggcat ttgcgaagcg acggactgga
    39601 aattacttat cgaccagaaa atctccggca tagtaatgct gaacgcggca acagttagca
    39661 ccagcaggcc gatcaagtaa aactcaacgt tattacgcca cgattttttc atagcagact
    39721 ccggttctga tgctgactcc acgccgtggc actaatgctg gcaacgatga tgatgccggt
    39781 gatcaatgtt tgccagtaag acgagactcc caataaattt agcccgtttt gcatcactgc
    39841 caacaacacc acgccgagca aagtaccggt taacgtgccg cgcccgccga gcaaactagt
    39901 gccgccaagc accaccgcag ccagtactgt cagctcataa cccagaagag aatcgggggc
    39961 gacagtcatc accgtccacg actgcactac acccgcagcg ccagacatca atcccatata
    40021 gccatagacg aaaagttgca gtttcagcac gctaaagccg atgcgactgg ctgattcgcg
    40081 gttcccgcca agggcataaa ttttgcgccc tacactggtg aaattcatga taaacgctgt
    40141 tagcagcacc accgtaatca tggcgatcag cggcagacca aggccatagt catagccatc
    40201 agcatcggtg tacttgaaca acataacccc ctgctcaaac cacggcggaa agtcgtacag
    40261 ccacacacct ttactcaacc ataacagcag gccatagaaa atattcagcg tcgagatagt
    40321 gatgatgata gaaggcaccc gtaggcgatt aaccagtaag gcgttaatta agccaagtaa
    40381 aatgccgatg cagcccgcca gtgcaaacgc gacagcgaag ttgccaccaa ttttttgcag
    40441 caacagcacc atgccgtatt gcgcaatgat ggtcattgct ggaaacgaaa tatcaattcc
    40501 accggaaatc aacaccacaa acaaaccaca ggcgagaata gtcaacatgg catagttatt
    40561 ggcgagatcg tacaaattac cgaatgtcag aaactcgtcg ctgcgccagg caaggccaac
    40621 aaacagcacc acaatcagta atgccagcca gaattcatgg cgttttttca gttcagccat
    40681 ttaccacctc cgcaatcctg gcctgagaac tgctgtcagg caggaagcta tgggtgatct
    40741 gacctttttg cattaccaaa atccggtggc tttgatacca cgcttcttca atttcatcgc
    40801 agatcatcaa taccgcaatg ccatgagccg ccagatcgct gataatgccg tatattcctg
    40861 ctttgttggc gatatcaaca ccgactgtcg ggctatcgag aatcaacagt cgtggcccaa
    40921 tcgccagcca tttggcgatc gatactcgct gcgcgttacc accagaaagc gtattcaccg
    40981 gaagatgagg atcagagact ttgatggtca atgccttgat tagcctgtcc accaggtcac
    41041 gaactttagt ttcactcaga aagccgaagc gatcttttac cttgtgaaaa acagtgctaa
    41101 tgatgttgtc ttcaatagat tgcgccatca ccaaaccgcg cgacatgcga tcttccgaaa
    41161 cataaccaat accgtggcgg atagcgtcct ggttactatg cagtgtaacc ggctggccgt
    41221 tgatgaggat ctcgccagca tccggtcggg tcataccaaa cagactcaga cataattccg
    41281 tgcgccccgc accaagcagg ccaacaatcg ataccacttc gcccgcttcc acccgcaagt
    41341 taatatttag atattcccca tgacgactta aattacgcac ctccagcacc ggggtgctgg
    41401 attttccctg ccacaactct cgcacctgat attcaaactc ctggccggtc atcaggaaac
    41461 cgagttgttt tgtggtcatc tctgcagccg gaaaagtccc gaccagctca ccatctttca
    41521 gcactgaaat acggtcggaa acttccatca cttcttccag acgatgactg acgaagacca
    41581 cgcagatccc gcgttcacgc aattgatgca ccacctgtaa taacccttgc acttcctgat
    41641 gggtcagcga agcggttggt tcatccatca cgatcaaccg tgcgtcctgc gccagtgcac
    41701 ggcaaatagc caccagctga cagcgggcaa ttgaaagctc agaaacgagt gtatccagtg
    41761 gcaatgtgac gttaatactg gtcattgccg cctgcgccac ctcacgcaga cgacggcggt
    41821 taacaaacag accgtggtga tagtgattca cggcgatatt ctcccagaca ctcaggttag
    41881 gaaataaaga gaggtcctga taaatcacct gaatcccctg cgccaccgaa gcggcgggtg
    41941 tcagctttga ccagtttttc ccaccaagcg taatttccgc tccctcatcc gggcgataaa
    42001 caccggacat aattttaatc aacgtcgatt taccacagcc gttttgcccg gcaaggcagt
    42061 gcacttcacc cgacataaag tcgagagaaa catcgcgcag cgctcgcgtg gcatgaaacg
    42121 ttttgttgat atgacgaagg gaaaggaaag tttccataac atcccctggc aataagaatc
    42181 ggggctttcg ccccgaagaa atggattaat aaagtgagtc gatattgtct ttgttgacca
    42241 gtaatacctt atggaaacgg ataattcgct tatccatatc tacgtcggcc ttacccagat
    42301 tctgcatttc taatccaggt ttgatctctt cacccttcag caacgtgctg gcgacggctg
    42361 cgagtgcgta acctgcagag gccggatcgt aagtaatccc ttcggtgata tcaccacttt
    42421 taatcagtga tgccgcctgt gaagggatca tcatgccata gactgcgact ttatttttcg
    42481 cccgtttctc tttcaccgca cgtcccgcgc caatcggacc gtttgaacca aaggagacaa
    42541 ccgctttcaa gtcaggatag gttttcatca ggtccagtgt ggtacgacgt gagtcatcca
    42601 cactctcggc aaccggcatg cggcgggtaa cttcatgcat atccgggtaa tgctctttct
    42661 ggtatttcac cagcaagtca gcccataagt tatgctgcgg cacggtcaaa ctacccacgt
    42721 aaatcacata gccgcccttg ccccccatgc gtttcgccat atgctcaaca tattcagcgg
    42781 caaatttttc gttatcaatg atttcaatat cccagttagc acttggctga ccgggggatt
    42841 cgttggtcag aaccacaatt ccggcatctc gcgctttttt gaataccggt tccagcacgt
    42901 tggcatcgtt tggcacgata gtaattgcat taaccttacg ggcgattaaa tcctcaataa
    42961 ttttaacttg ttgcggagca tcagtacttg aaggccccac ctgtgaggca ttaacaccaa
    43021 aggctttacc cgcctcaacc acaccttcgc ccatgcgatt aaaccacggc ataccatcga
    43081 ctttagaaat attcaccacg actttttccg ctgcctggag cggcgcagaa attagcgcag
    43141 cgcctaataa cagcgaagac accatattga taacgaaacg tttattcatc atttcagcct
    43201 cattgcaggg tagtcaggaa gtgttcgccc aaattagcgc gctggagaga ataagaattt
    43261 tcgaatattt ttatgcagaa taaacagccc tatttcaggc agctattaaa aatatggtta
    43321 taaatatgga caaaacgggc agcaacaata agtgtgacat tcatcacgta aaaaaatagc
    43381 aaaataaaca aaattagcaa tattttatgt gtgtgagttt atttcgcgta gataaatagt
    43441 tatctacaca acgtcaataa gggcatattc ttcaaatata acgtccattt ctggtgaata
    43501 gcttccttct gcctggaaaa aacgacggtg ttcattacgc caatattcaa gactgaggtc
    43561 accttcacct tccttacggg caagctccac ggtgacatca gaaaagcgca gtaaatgcag
    43621 gccaatcact ctgataacac atacaggctc accacggcta ttttctacaa tcttatactc
    43681 accaatcatc ggaaatgcgt tgtcttcaat acatcccgcc agcgaaccac aggaagctgt
    43741 tttaacacca gtcgcaatca acgttgcgag gtgatcggcc aacgctgctg aatcgccaaa
    43801 agtacaaaag gaagcatggg gatatttttg tgagaatttt tcttttatgg tcatggcaat
    43861 atttttgcga ttacggaaaa acaaatcaca tcttaattac tgttggtaac catcagcaag
    43921 agtatcattg gtttttcgat actgaatctt ttttatccga agcaggtcac atttatggcc
    43981 tgataaatac ctttgttaaa actcactttt gtgtatttct ctcaaataat cccctggaaa
    44041 ttatgccgcg agattataaa gttcaggatc attcattagc aaaccattcc cgccccactc
    44101 cctggggctt aaatctctta caattcaccg ttgcaggcgt ttcaaatgcg catcaacctg
    44161 ccacactccc ccacaccagt tgccctttat ggaacgttgc ggttctcggc gagatacgcg
    44221 ccaccgcctc ggcggaacag gaggcgtcca ccagcacaaa gctggcatca tcctgcgctt
    44281 ttggccatac acgctcgcct ttttcattca gcggcaatac atcgccagtg gctagaaaca
    44341 gcgaacggga gaggttctgt tcgttaggac gaatatagag ctgcgcgtag agattggctt
    44401 tttccagcat gtcgcccaga ccataaggcg accagtggtc gataacactg tcagtgccag
    44461 tcatcacttt tacgcctttg tcgtgcaact gtttgagcgg catatgcagc gtgccaatcg
    44521 gcaccgtcga ggcgatagaa atttgctgcg ccgccatccg attcgccagc gcatcgacct
    44581 gttgttcgtt gagcgtagcc agcgcaaagg cgtggctgat ggtcagctta cctttcagtt
    44641 gcggcgtttt ctctaccgtt tcaaccatat aattgatggc tgccacgccc gacggagtgg
    44701 tttcgtgcag gtgaatatcg acacctttgt cgtagtccag cgcaatctga aacatggtgt
    44761 cgagggattt ttccatcgcg ccatcgacac tggtcgggtc cagcccgccg acgtaatgcg
    44821 cacccgcctg catcgcttcg cgcattaagg gttccgattt cgacagcagc aaaccgtgct
    44881 gcgggaaggc gacgatttca cactcgaagc ccgcctgacg tcgcgccagc accgcctgca
    44941 aattctgcag atttttcagg ccggaaaccg gttcgatatt gcagtggctg cgggcaatgg
    45001 tggtgccttt cgactgcaat agatcgatca gtttttctgc ccgttcctgc gtgtacggtt
    45061 gcagttccgg cagcattttc tgctcaagct tgatcatgtc ctggatagtg gtgcctgccg
    45121 gacgattgag cgagcgccac ggcccgccgt agaaggtttt gtcgagatga atatgcatgt
    45181 cgcgagtggt gggtagcatc aacttaccgc cagcgtcata gtgcggcagc gtggcgtccg
    45241 gatgctgctt gttctcgcgc agggcgacaa ttttgccgtc ctgaatctcc acggtctggc
    45301 gcgccgtgcg ggtctgcacc gccacaccgt tttcgtagtc aaaaccggtt tccagcagca
    45361 cgttatcgag atagtaatgc gggtcagtga ttttcatggt gttgttcgct tcgcagttta
    45421 gggtgccagc taccgccgca tgggcgagcg gcacagaggt accaaacagc gcggcggcgg
    45481 tgaccatctt accgctctgg ctcagaaatt cacggcggct attgctttct ttcatcctac
    45541 gtccttacgt tttaataata tgggtgcccg tttcaccgcg cagcgccgca ggcaggcatt
    45601 ccggcgtggt gataatcact tctttgccgc cctgctccag gaacgtcagg ctggcgatga
    45661 tttttggcaa catgctgccg ggcggaaaat gcccttcctg catatagcga gtcatggtgg
    45721 caatatccac ccgatccagc gcctgctgct gcggtttacc aaagtggata cagacttttt
    45781 cgacgccggt ggtgatcacc agaatgtcgg cgtgaatttc acgggccagc agcgcggtag
    45841 agagatcttt gtcaataacc gcgtccacgc tttggtaatc ccccgcttca gtacgcacta
    45901 ccggaattcc accgccgccc gcgccaatta cgacaaaacc ttgttggatc agcgccttaa
    45961 tggcgggtgc ttcgacaata cgtttcggtt ccggcgaggc gaccacgcgg cgatagcccc
    46021 gtccggcatc ttcaacaaaa caccagtcag ggtttgcctt ttgtagtttg tcacgctggc
    46081 tttcactgaa gaatgcacca atgggcttgg tgggatgggc aaaacctgga tcgtttttat
    46141 ccacttccac ctgagttacc acggtgacgg cttttttctc gccgtgacgc gccagccggt
    46201 tattcagcgc ctgttggatc agatagccga tgccgccctg cgtatctgcc acacagttcg
    46261 ccagcggcgt taagggcagc ccttcgcgct cgtgggcaat ctccgcacgg cgtaaatcca
    46321 gcccgacctg cggcccgtta ccgtgggtca gcacaatgtc gtaatcggaa gccagcattt
    46381 ccagcaccgt atcggccacg gctttcaccg cctccgcctg atgctcaatc gactggctgg
    46441 cgttatcttt gataatgctg ttgccaccaa tggcaacgac cacaagctct ttcatgttgt
    46501 tatgtccgct acagagggtg attaggttat gaccaacgcc agactgcctg atgcgctacg
    46561 cttatcaggc ctacaattct cgggccatcc gtaggccgga taaggcgttc acaccgcatc
    46621 cggcataaac atgtaagtca gacgccgtaa cgttcgcacc agccgagaat ggctttctcg
    46681 aagcaggcga atggtggatg cacaatgccc gcgccaatca tgccgacgcc cgcatcttta
    46741 tgggcgatgg cggtgttgat caccggcaga atgccgctgc tacccacgcg ggtgatgtcg
    46801 attgccgacg gcacgcccat aaaaccgagc agcggaatgg tgacgttggg gttttcgcca
    46861 agggtgattt cgcgcatctg acgggagaaa tcgatagcct cttccaccgt gccgcccacc
    46921 agtgcgacga ttgcaggcgc ggtcgccatg gcaaacccgc caataccgta ggtttctgtg
    46981 atggcgctgt cgccgatatc cagccccgaa tcttccggct tataaccagc aaacatcggg
    47041 ccgatgacct gctgcgctgg gccggtaaac cactgccccg gcaggccgct gacccgcagg
    47101 ccgaactcga cgccgttacg cgccatggtg gtgaccacgg tgctgtattc gatgccgtgc
    47161 gccgcatcca tcgccgcttt acacatcgcc atccacgtcg ggccggagaa gtagtcgctg
    47221 ctggcgacaa actcgaacac ttcgcgctgt tgctcgacgg aatagcctgc ctgaatgatc
    47281 cccggcgtca gtgcctgaat cagcagcgtc gtcccggcgt tattgcggtt atggcactca
    47341 tcgcccatat gcagcgcctg cgccagcatt aagcgcagat cgatctcacc gataattttc
    47401 atcgcgtcgc gcagcattgg cccctgcaca tcgcgcatcc agttgaggcg gtcgatcacg
    47461 ctctgatcgt tggcacccat acgcaaaatc ttcgccatct gctcgctcat attggtataa
    47521 gcgatgttgc cgtaggtttt gtttttcacg atgtgcataa acatcgaggc cgaggtaacg
    47581 cccgccatcg atcccacgca gtcgtgttcg tggcacggcg agaaggtgat ctccccggag
    47641 gccgccagtt cagtcgcctc atcgagatct ttcgccagcc cttcgaacac caacgccccg
    47701 gtgaccgcgc ctttcatcgc gccgcacatt ttttcccagg tgatcggcgg cccggcgtga
    47761 agaatggttt tcggcgtcat gcccggcacc acgttaatcg cctgatcaaa accaatcagc
    47821 accggatgcg actggataat gcgctccagc gcctgctggt tagcggcggc gattttgtct
    47881 gccagcggcg aatcggcaat gttatccagc gcctgcacca cctgcatatt gccctgcccc
    47941 ggcggcgtcc agtcgagttg agtcacttct acatgctgct ttttcaggtc atcgctaaac
    48001 atggcgatac cgacgttaat aacgttcaac ggttggctaa acagtgactg gctcatcagg
    48061 cttcctcccc tttgcagata aattcacgcg ccagcaatcc ggtattggtg ctgctgctcg
    48121 ccagaatcac tccggcatca agcagcatct ggctttgttg ttctaatgat ggcgtatcga
    48181 gatcggtgcc cagcacatag gcgagaatga tcaactcgcg tccttcggcg gcggcgatcg
    48241 ctttcgcttc tttgatcgcc tcgatggtgg agccgaccgg atcttcatgc gatccaaatc
    48301 cgagcacaaa atccatcacg atcaccgcca cttctggatc gcgcgcctct tcgatcaagc
    48361 gactgatgcg gttggtgggg tcaatcattg gatgcggctt gccgttggtg aagtcgtcat
    48421 cgccaaagtc gaggaaggtg tgtttgatgc tacggttgat atcttgcagg cggaattccg
    48481 gatcgggctg aatgttgctg tagacatcgc cgtgtttttc catcaccgcg aacatggttt
    48541 cgtcacacag cgtgccgcca cagaacaggc cacgaatgta cttctgctgc ggttgcaggc
    48601 gagcacgtac gtcagcaatc aacggctggt tcagcgtatg caggtcgaga tgttcctgtt
    48661 tcacgccgga gagcatcacc gctttcaatg ccgcctcttt gctgccgcgg gcaaactgta
    48721 gcccctgctc atccactggc gtttcgccac gaccgaggaa acagaccacc accggcttgc
    48781 ggcaggcgcg cgcgcgttcc agcactttgc gggccaccgc aggcgcgggc ggtttggaga
    48841 taagcacaat gatttcagtt tgcggatcgt tttccagcat gccgatggcg tcgagcatca
    48901 tcaggccgcc gattttctcg ctcaggtcgc gcccgccggt gccaatcagt tgcgaaacgc
    48961 cgccgccaaa ttcatgaatg cggacgctca gctcctggct gccggtgccg gatgcgccaa
    49021 caataccgat gttgccgcga cgcacggcgt taccaaaaca gagcgccgcg ccgttaataa
    49081 tcgccgtgcc acagtctggc cccatcatca gcagcccttt ttcgtgggcc agttgcttga
    49141 gcgccaattc atcttcaact gagacgttat cggaaaacag catcacgttg agatcgtttt
    49201 gcagcgcctg acgcgcttcg cgagcggcaa acagaccgtt gaccgaaatc accgccaggt
    49261 tactttccgg gatatgcttt ttggcgctgg caatagtggc gtaacgcgcc tcatgcgagc
    49321 cgctttgcgc tttggtgttg aacagttctt caatctccac cagcaactgt tcgttgtccg
    49381 cgcccgcttt gccgttgatg acaatcatca ggtcgccgtt tttcgcctgt tccagctccg
    49441 gcgtcagcag tcctaaattt ttcagtacac ctttgttcat ttcggtcgcc atcgccacaa
    49501 atgcctgctc gacgccgtcg agtttatttg cacgcgtgga gatagacatc agcgagacag
    49561 aatcaaaata ggtattcggt ttaatgacta ttttaactga cataattccc tccgtaggtt
    49621 tggctcagcg cacaaccatc ggccatgcca tacagcatgt cgcagccgga actggagcca
    49681 atatgtttaa ttttttctat tgcctgagtt gcgttcccag ggatgtcata aataatgttg
    49741 tgaataaaac gatgaatatt ttcccggcaa cgttgttgta atgcggcttc cagcgttata
    49801 gcacttaata atgtggtgtt atttctgccg cgctgcatac cgagataaaa ttcctctttg
    49861 tatttttccg acggatgccc gggaataaat aaaataagcg ccagacctgt taaataatcg
    49921 tcggctgatg gcgtaaggcc aatccctaac ccaatcatac tggcgaccgc tgcggcgata
    49981 ttttgttttt cgtttactgc ctgaataact gcccgtcgtt taatatgtgg ttctttattt
    50041 aacgcctgat aaaacggatt atcgccgcga taattaaaca gggtttcatt ttcccccagg
    50101 tgctgatgaa taatgtcgtg ccactgtagc caggcgataa ggtgaaaatt cacagcggtc
    50161 atttgcaagg ttggggacag ccaacgccga caacgactca tctctatatg aagatgttga
    50221 ccaaccgtaa taccttgatc gtcaaactga accttatcac catgccggaa cagatcatca
    50281 aagtgagtga gtgccaaccg acaactgttt ggcgcattgt cgtactcttc acaaagcagc
    50341 gtcagcaact gctggcgagc cgggagtgcg atatttaccg ctctggcaaa gacctgttca
    50401 acctgtcctt gcccccgctg cgccagaaaa tccgcatccg cagttagcgc ccacacacac
    50461 cgtctttctg gtgtgttgat agcaagcctc ccggtttatg cacctgcggc aatcagtaac
    50521 tgcgcaatct cttcaaagcc ccgttcccgc gccagttcca gtggcgtttt gccatattta
    50581 tcggtcagat gcgggctggc accgtgttcc agcaataact gcacaatcgc ctgctgttta
    50641 ataccaccat cattaagcac aatcgcttcc agtagcggcg tccagcctac atggttggtc
    50701 tggttaacgt taatctccgt atgcgccaaa agctctttta caatacttaa atggcctttt
    50761 tcacaggcag gcgtcaggcc gacaccgcca aaacgggtta cgcaattaag atcgggttta
    50821 gccggtaaaa taattcgtag tagcgttaaa tcatcgttca gacagctaat taaaaaagga
    50881 tttaaacagg tatgatcttg cttattaata tccgctccgg catcaattaa tgcctgaacg
    50941 caagcatatt gctgatataa acttgccagc gtaattgccg ttttcccctg acgatcgcag
    51001 gtattaatat cgacacccag cgcaaggcag gtttttactt tatcgatatc accctgttgt
    51061 gcagccaata aatagtcggc aggtagattt tttatactca ttcacattcc cctggttttt
    51121 gtggctgttt tcgatgaatt gagaatagca acgataatct gataaaagaa cccgcttaaa
    51181 tttaattcgc tgtatttcgc ctttttgttg aatgatgttc aacaatcttt tcgcagtgtg
    51241 cggtagtgca atatttgttt ctttgacccg ataccctttc actcaatcag gttttatttc
    51301 tctatagtct gcacatgtaa gagtagcttt cgtccatgtc taaccagagg ggaacaagat
    51361 gaacggactc acggcaacgg gtgtcaccgt cggtatttgt gcagggcttt ggcagttagt
    51421 gtcttcacat gtcgggctct ctcaaggatg ggaattatta ggaaccattg gcttcgtcgc
    51481 cttttgcagt ttttatgccg caggcggcgg taaatcgggg tttatcagaa gtctggcggt
    51541 taattattcc gggatggtgt gggcgttttt cgccgcgctg actgccggtt ggctggcctc
    51601 ggtgagtggg atttctgggt tctgggcgag cgtgattact accgtgcctt tctcagcggt
    51661 agtggtctgg caaggacgtt tctggttact gtcattcatt cccggtggct ttctcggcat
    51721 gacgctgttt tttgcctgcg ggatgaactg gacggtgacg ttactgggtt ttctggcggg
    51781 taactgcgtg gggataattt cagaatatgg cggacaaaaa ttaagcgaag cgacgactaa
    51841 acgcgacggg tattaaaagt tattcaaaaa tgattatcta tctttaatcg atacctctac
    51901 agtcatggct ggagaaattc atattagata atggcgggaa tggtttaatg aactcagttt
    51961 ttaccgaaga gaatctgttg gcatttacta ccgctgcgcg ttttggcagt ttcagtaaag
    52021 ccgcagaaga gttgggttta accacttccg ccattagcta caccatcaag cgaatggaaa
    52081 cagggctgga tgtggtgctg ttcactcgca gtacccgcag cattgagtta acggagtccg
    52141 ggcgttattt tttccgcaaa gctaccgacc tgctgaatga tttttatgcc atcaaacgta
    52201 gcatagatac tatttcgcag ggcatagaag cgcgtgtgcg catttgtatt aatcagctgc
    52261 tttatacgcc caaacacacc gcgcgcttat tacaggtgct gaaaaagcag tttcctacct
    52321 gccagattac cgtcaccacc gaagtgtata acggcgtctg ggatgcgatt attaataatc
    52381 aggccaatat cgccatcggc gcacccgata cactactgga cggcggcggt attgattaca
    52441 ctgaaattgg cgcgattcgc tgggcttttg ctatcgcccc ggatcacccg ttagcctttg
    52501 tgccggaacc catcgccgaa agccaactgc gtctctaccc taatattatg gtggaggaca
    52561 ccgcgcatac gattaataaa aaagtgggct ggttgctgca cgggcaggag tcaattctgg
    52621 tgccagattt caacaccaaa tgtcagtgcc agattttagg tgaaggaatt ggttttttgc
    52681 cggattacat ggtccgtgag gcgatgacgc aatccctgct ggttacccga caaatccata
    52741 atccgcgcca ggattcgcgc atgttactgg cgactcagca ttctgctacg ggtcaggtga
    52801 cgcagtggat taaaaaacaa tttgcgccaa atggcatatt aacggggatc tatcaggatt
    52861 tactgcatcg ggagaattaa tcgctgccaa cctcccgatg cgctgtgaat agcggtttac
    52921 atcaaccacc tgctttcatt acccattcag agataaattt attacccggt actggcggag
    52981 agtacaaata gccctgcaaa aagtgaacgc ctttaccgat cattaaatcc gcctgctcct
    53041 gggtttctac cccttccgcc acgatactca gaccaggctt acgcgctagt tcgacaatat
    53101 tgtccacaat atgaccggag atttcgtcga cactcgccat ttgcacaaat gacttatcga
    53161 tcttaataaa atcgaccggg aacgcctgca agtaacgata ggtcgcataa cccgtaccaa
    53221 agtcatccag cgcaaaggta atgttgtgct gatgaaggct gtcaaatatc gctctggctt
    53281 ctggcgttac cggaataggg ttacgttccg ttagctcgag aaccagcttg attttatcgt
    53341 tacctaattt cttaaccagg ttcagactct ctttttcaaa tcccgctgcc aaaaaacaac
    53401 ccgccgagac gttgatgcca atatggaaat tgtccggcag caaatgtttt accggcatca
    53461 gaatatccgc agtctgtttc atcagttggc gggtcattat gacaataaga ccggatgact
    53521 ccgccagagg aataaactga tccggtggga taattcccgt ttgtggatgt tcccagcgga
    53581 caagtacctc acagcccgtc agtaccccag tctgcgcgca gaaaaccggt tggatccacg
    53641 gtttgaattc gtggttttcc aacgccagac tgatggcttc aggcgtgacg atatggtgac
    53701 aacggttatc agggatataa aaattatttt tatttcccgt gccactgatg acttgcggat
    53761 gatattgaaa aaagatatcc cgccagaagg taatatcact ctggatgcct agtttttcat
    53821 acagctgctt cttttgatgt gaaattgtct ttgcactgcg atttctgtac tgtgagattt
    53881 ctgtaaccga cattccctcc attagcagct tgagtaaaag ccgttctttt tcggacacga
    53941 taccaggcaa cgtcaaattc accgttgccc cgaactcttc caaaaaaaca ctcaaatcat
    54001 gcgaagccat cgaacacacc ttgattaaat cttgtgcgcc agctaaatct gcgagtcttc
    54061 actctgactg ataaagctgg ctgcaagaaa atgacacaga gagccgaatt atctcccgtt
    54121 cggctctctc tccttttact cattattcag atcagaattg ccacttaatg ccaaccattg
    54181 cagaggtgtc attatatccc ctatcggcaa cctgaacgcc cacattcccc cagacattca
    54241 ggttggcatt tagctgtcct tccaccccgg tttttatctc agcaatattt cgggctccat
    54301 cctgagtgac ggacacgcca tccatactgg tgctgaaatc cttactatta tgcagccagt
    54361 tcacttctac aaacggctgg aactcgcggg atttaccgtc atccatttta tggtggctct
    54421 tcagccaggt ttttacgcca agtcgggttt gaacattgcc atcaccgttg ctatgaacga
    54481 gggttccgtt gctttcgcgg tgcttatcgg ctttgactcc catccaggta acctgtgctt
    54541 gcggctgaac ataccattca ttacgcgttc cctggctgcc attaaattca gctaatttgt
    54601 gtttgtatcc agcttccagt gaagcggtaa atccttttga tttataggat tcactttgta
    54661 agtcatcccc tttcactgtg ttatcaaacc agctgtactg cgcccaactg tcgagatacg
    54721 cgccattacg cgactcgtca tcggcatacc aggtggcata gaggccggtg ctatatccgt
    54781 tcacactcgc ttttgcacga taaccggttc gcgaggaaat ggttttgctg tcgctgttgc
    54841 catatcccgc catgacccca acatgccagc tgtcgctgcc gttttggctc cactgcgcga
    54901 catcgcctcc cagttgcaga acatagcgat tgctttgggt tttcagctgg ccgctgccat
    54961 cacgccattt attatgacca ccttcatggc gcatccacat agtggtttgt ttctgctcac
    55021 ccgtcaccat gtcggtatag tacgtattac ccagacgctc atgcagacgc gtggtgaaca
    55081 ttgtattcgc tgctgcaagg ttcgcaatgt agctacccgc ctccggtcgc aggtcattat
    55141 ccacattcag atccggaccc ggcgtaggtg tcgggttagg gttcggctct ggattcgggt
    55201 ttggctccgg attcggcatc gggtctggct ccggctgcag ttcaggagaa tcactgccgc
    55261 tggtcagata ccagttacca ctatttgccc cttgtccacg cgcgagagtg tagtcatacg
    55321 cccccgcaac gatacgacca gcctgaacaa attcgccctc agacttaccg tctacatgga
    55381 taagttcgat accgttaagg gttttcgcac ctgtaccgcc agcgttattc accgtaacgg
    55441 cagtcgtgcc gctagtatcg ccctcgacaa ccaatttatc ggttactgag tcatcgccat
    55501 tcagtaccgt attcattact agttgaccgt tattaccgtg gtagttcccc ttcaccacta
    55561 acacattgcc agcttgctgt cctgacattc cggtcatcag cgtaccgttg ttggtcagat
    55621 tgccgcccag ggtgtaattc acagacgcag ccgctctttt ttcagcgcta ttattcaacg
    55681 ccagggtacc gttgttctca acgtttgcgc tacccaatcc ctgcgcatga cttaccagga
    55741 cattagccga attagcaatg ttcacattcg ccgtcaggtt gctgttatcg cccgcgatgt
    55801 ccagcgtgcc gccctgaata ttcagctctc cgctccccgt taacgaaccg gttgaagtgc
    55861 ccccgttggt aaccgtcaga ctgccgccat ttaagctcag cagtgaatcg gcgctgccat
    55921 tgagcgtgcc cacggtctgg ctgtgaccat tcatatccag ttcagtctct gccgccagac
    55981 gcagttcatg ggtattaccc aacacgttat cgttattcaa caaaagcgtc ccactgcgca
    56041 gatcggtaac accggtatag tcgttgtctt tgttagaaag cgatacggtc tgacccttct
    56101 ggctgctgaa tgccaggtca ccactgccgg taatctttgc gctgagatcg gcggcattct
    56161 cgcttttacc gttagagctc agcaccagag catcgctgtc ggtagcgtgt aaatcaagct
    56221 gggtcaggcc gtaaccgata tacagaccgt cattgtttac cccactcgtc agacgatagt
    56281 cgtaagtgcc gacggccgct ttttgcccat tctgctgaat atcacgttgg gcactgctgg
    56341 agaggatttg cccattttta tcctgcaatt gcagattgcc cgcatcgccc agaacttccg
    56401 caccttgcgc atcaaccaac ttaatggtgc tgttaccatc atcgacttca gtgagcgata
    56461 acgcagagtc aatatcacga gatactgagc ttacaacgtc actgtcagaa acctgaatca
    56521 caccctcgcc gcgcaaatcc agcgttttac taacgttgac cgtcccttct gtcatctgtg
    56581 cacctgctgt cagggcacca aaatcaacgg ttccgccgga gaatcccaaa gtgctaatgg
    56641 agtgagcgcc cttacccact attacctggc ttccctgacc cgcagtgatc ccgcctctct
    56701 gcagaaactc agcattcttt tccaggttaa atgtcgtgtt ctggaacgcc agtgaaccct
    56761 ggaaccagtc tgtataagca aactcatcga aggagaattc gtgattcccc atgtttattt
    56821 caacaatacc tctaccttga agatcgttat caaataccca atctttattg gcattgattt
    56881 gcagtttgcc atcaacatcc aggtccgcac ggccgatgtt cttgtcttca gaaataaccg
    56941 cgacagcacc agaagcgaca tcaatatatc cgctgaatgc atagttattg ccggtcagtg
    57001 ccactgtccc actgtcgata cttattgtac cattgccgga aatcttattg gcaaaggtca
    57061 tgctatcgtc agccttcagg tgtaatttcc cgcggttgtt gatctctgct gtacctatat
    57121 tttgggtgat ccctaaagcg ccattttgct caattttgac tttaccacta aaggcagagt
    57181 tatcaccggt taagtaagtc gtgccattga gcgcattgat atagcccagg ccctcgctgc
    57241 cactgacaat atccgcggaa atagtcgcgt ttttatccgt gtggtttaag gtgattttct
    57301 tatcggatgt ctttgcccac acaaactcaa ttttttcggc atcaataatc cctgcagcct
    57361 tcgcagcact tccttcctgt gcacctaacg ctaactcaga gttggtgctt aaactaattt
    57421 tcggtgcgga tattttacct ccatctgata cgactaatgt tgtatcagaa cgttggtcgt
    57481 aataacccag gctaatatgt tcacgagcat taacttcacc gccgttatta actgacaggt
    57541 actgattacc accgtaactg ccgtaaacgc ccataaagaa ggttttagaa tttacctggc
    57601 tgccttcacc actgacattt ccctgcaaat aggtcagacg gctggtgccc ttaacatgga
    57661 ttttgccgcc gttggagaca ttaaaatagc cgcgatcgtg tctcatgcct gagtttgact
    57721 catacaaatc atcagcatat aactcgctgc cagcattgga gacattcagg aaccccgtac
    57781 ctttcagcaa aaagttaagt gggccattga tagttacctt tcctccggta atatccaacc
    57841 caccaccaga agcgagagtc agacgcggac gggaagtatc tgtcgcattg attaccagat
    57901 tggcattgtc agtcaccgta atacggtaat cgatgctctg gttctctttg ccaaaatcaa
    57961 tagttttatc ctggtttacg atgacatcat cggcgaatac cagagatgaa tataaactta
    58021 tcgcgctaat taaaacagcc cgggactttt ttccttttac tctgctcccc agctccgagc
    58081 agacgctcca acagttacgg gacctgttcc actttagccg atatatttta ttcatcacaa
    58141 tctccagcaa tacatccaaa taaaaccaag attgcataaa cagcaacgca ttaaatgcca
    58201 ttctggttac tgttcatcgc atataaaaat tcaaaaattt tgcatagcat tggcaaccac
    58261 atatctgagt atgtagttca gagggtaata aaatagcaag ttattactta aaacttattt
    58321 taagttaaca ctcttaactt tatcattaca ttcctcccag tctctaatac ctgcgcatta
    58381 tatgtatcca ggaactcaaa tcaactcaaa atataaacaa aagcaagagg aatacattta
    58441 atatacagcc gataaaagcg gtaactctta ttaggtatca aaatataaga atcgtcttat
    58501 taataaatat ataacactat aggactgaat ccattcagct aatacaaaat aaaatcgtta
    58561 attaataaaa tataaaatca tgaagtgata caagaggaat tgcaggatat gacatgacaa
    58621 tcatatataa tatatgcata attttttaca gcgagtcaat gattcccgcg ccatatgcag
    58681 acatggcgcg gttttatgca aaaacaatca cgcgtccggg aacatcacgc tatggcccgg
    58741 cgcttcacga tggagatgaa taaagttaag gtgccgctcg tactggtcaa gaatatcggt
    58801 gatcacctgc tctttgctgt agtccatcag gtcgttgccc tggctgcctt ctaacaggaa
    58861 ggtttccagc cggtagtagg tcgatttacc gctacgtgcg cggtaggtaa agcccggcac
    58921 cgaatattgc tgcggccaaa tctgatagac aaagttttgc tcttcgccca tatgcaccaa
    58981 caaatccaga tgccccaact gttgtccctc ttccggcggc aggcttttta gctccacgta
    59041 cgcgccgcgc aaccgcagct cctgcgccac ttcttccatt gccgggtaac agaccgtctc
    59101 catcatctgt ttagtgtaac gcgtgcccgg ataattcatc aggcgcgaga gacgtttttt
    59161 ccagctcagg cgatcctgaa gccccagcgg tcgcggcgcg gtatcgcggt tggcactttc
    59221 acggcggtaa tcttctacct tcagagattt atacaacccc gccatcacga agaaaatcac
    59281 aaagctgaac ggcagcccca tgattaccgt ggtgttttgc agcgcggata tcccgttggt
    59341 catcagcatg ccgagcgtca gcagaccaat cgccaccgac cagaagacgc gcagccagcc
    59401 gggggcgtcg ctgttgatat ctttaagctg cgaggtgaaa ttccccagca ccagcgcacc
    59461 ggagtccgcc gaggtcacat aaaacagcag gccagtaatg gtggcgacgg aggcgctaaa
    59521 ggtaaacgcc ggatactgcg ccagcaggct gtagaagccg cgctccggat ggaccatcgc
    59581 ttcctcggca aatgccgcgc cgccgtggat gatttcatac agcgcgctat tgccgaacac
    59641 cgagagccat aacagcgtga aggtaaacgg aataatcaac gtgcccagca cgaactggcg
    59701 aatggtacgc ccacgcgaga tacgcgccag gaacaagccg acaaacggcg accatgccac
    59761 ccaccatgcc cagaagaaga gcgtccagtt attcatccac tcaactggac ggtcgaaggc
    59821 aaaactgttg agcgtcatgc ccataaagcg attcacatag tcgccaacat tcagcaccag
    59881 cgcattaagc aggaacgagg tgtcgcccat aaacaatacg aacaggatca atcccagcgc
    59941 cagcgcaacg ttaagttccg ataacacacg aatcccctta tcgacaccgg aggtgacaga
    60001 gatcgtggcg attatcaccg acaaggcgat cagtgccgct tttgccgcca tcgaatccgg
    60061 aatatcaaac agtacgctca agccatagtt aagctgcacc acaccgatac cgagcgtagt
    60121 ggcaataccg aagatggtgc cgatcaccgc tgcaatatcc actgagtgcc ctatcggccc
    60181 gttaatccgt ttaccgaaga tcgggtacag cgccgagcgg atggtgagcg gcaaattata
    60241 acgatagcta aagtatccga gcgccatgcc catcagcgca tacatcgacc agccggttaa
    60301 gccgtagtga aacagcgtcc agaccatcgc ctgacgcgcg gcctcaatcg tctgtcccgc
    60361 gccttccggc ggctgcatat actgcgttac cggttcggct acggagaaga acatcaggtc
    60421 gataccgatc ccggcagcaa acagcatcgc cgcccaactc agcaggctga attccggttt
    60481 ggattgttct ggcccgagct tcaccgaacc aaaacgcgaa caagcgatac agaccacaaa
    60541 gacaatatag agcgttgccg ccagtagata gtaccaaccg aaggttttag aaacccagtc
    60601 cagcgtgcgg ccaatccaca gggccgagaa gtcgcgaaac aggatcgttg tcagggaaaa
    60661 caacaaaatc agtccggcgg aggtgtaaaa caccaccgga ttgattttgt ccttttccct
    60721 gctgtgtgaa aggtctgtca tccagtatcc ccactgttat tgttaccatt taaaatcaaa
    60781 ttcgtaacaa ttaagacaca ttttatattg aacgtccaat caataaccgc tttaatagat
    60841 aaacaacgct gatgaatgga gtggcgaaaa tgcccaaatt ggggatgcag tcgatccggc
    60901 gcagacaact gatcgacgcc acactggaag caataaatga agtgggcatg cacgatgcaa
    60961 cgatcgcgca gatcgcccgc cgtgcaggcg tttctacggg gatcatcagc cactatttca
    61021 gggacaaaaa tggtctgctg gaagcaacca tgcgcgatat caccagtcag ctgcgtgacg
    61081 cggttttgaa tcgattacat gcacttccgc agggcagtgc agagcagcga ttacaggcga
    61141 ttgttggcgg aaacttcgat gaaacgcagg tgagcagtgc ggcgatgaaa gcctggctgg
    61201 cgttctgggc cagcagtatg catcagccga tgctctatcg tttacagcag gtcagcagcc
    61261 gccgcttgct gtcgaatctg gtgagcgagt ttcgccgcga attgccgcgc gaacaggcac
    61321 aggaagcggg ctacggcctt gccgcgctga ttgacggatt atggctgcgc gcggcactga
    61381 gcggcaaacc gctggataaa cctctcgcac actcgctgac ccgtcacttt attactcagc
    61441 atttacccac cgattaaccg aggagacgtg atgtcccgaa tggcagaaca gcagctttat
    61501 atacatggtg gttatacctc cgccaccagc ggtcgcacct tcgagaccat taacccggcc
    61561 aacggtaacg tgctggcgac cgtgcaggcc gccgggcgcg aggatgtcga tcgcgccgtg
    61621 aagagtgccc aacaggggca aaaaatctgg gcggcgatga ccgctatgga acgctcgcgt
    61681 attctgcgtc gggccgttga tattctgcgt gaacgcaatg acgaactcgc aaaacttgaa
    61741 accctcgaca ccggaaaagc atattcggaa acctcaaccg tcgatatcgt taccggtgcg
    61801 gacgtgctgg agtactacgc cgggctgatc ccggctctgg aaggcagcca gatcccgttg
    61861 cgtgagacgt tatttgttta tacccgccgc gaacctctgg gtgtggtggc ggggattggc
    61921 gcatggaact acccgattca gattgccctg tggaaatccg ccccggcgct ggcggcaggc
    61981 aacgcaatga ttttcaaacc gagcgaagtc accccgctta ccgcgttaaa gctggctgaa
    62041 atttacagcg aagcgggcct gccggacggc gtatttaacg tgttgccggg ggtgggcgcg
    62101 gagaccgggc aatatctgac cgagcatccg ggcattgcca aagtgtcatt taccggcggt
    62161 gtcgccagcg gcaaaaaagt gatggctaac tcggcggcct cttccctgaa agaagtgacc
    62221 atggaactgg gcggtaaatc accgctgatc gttttcgatg atgcggatct cgatctcgcc
    62281 gccgatatcg ccatgatggc aaacttcttc agctccggtc aggtgtgtac caatggcacc
    62341 cgcgtcttcg ttccggcgaa atgcaaagcc gcatttgagc agaaaattct ggcgcgcgtt
    62401 gagcgcattc gcgcgggcga cgttttcgat ccgcaaacta acttcggccc gctggtcagc
    62461 ttcccgcatc gcgataacgt gctgcgctat atcgccaaag gcaaagagga aggcgcgcgc
    62521 gtactgtgcg gcggcgatgt actgaaaggc gatggcttcg ataacggcgc atgggttgca
    62581 ccgaccgtgt tcaccgattg ccgcgacgaa atgaccatcg tgcgcgaaga gatcttcggt
    62641 ccggtgatgt cgcttctcac ttatgaatct gaggacgaag tgattcgccg cgccaatgat
    62701 accgactacg gcctggcggc gggcatcgtg acggcggacc tgaaccgcgc gcatcgcgtc
    62761 attcatcagc tggaagcggg tatttgctgg atcaacacct ggggcgaatc cccggcagag
    62821 atgcccgttg gcggctacaa acactccggc attggtcgcg agaacggcgt gatgacgctc
    62881 cagagttaca cccaggtgaa gtccatccag gttgagatgg ctaaattcca gtccatattc
    62941 taaccaggag gtttatttgc aatttgacta catcattatt ggtgccggct cagccggcaa
    63001 cgttctcgct acccgtctga ctgaagatcc gaatacctcc gtgctgctgc ttgaagcggg
    63061 cggcccggac tatcgctttg acttccgcac ccagatgccc gcagcgctgg cgttcccact
    63121 acagggcaaa cgctacaact gggcctatga aacagagcct gaaccgttta tgaataaccg
    63181 ccgcatggag tgcggacgcg gtaaaggcct gggtggatcg tcgctgatca acggcatgtg
    63241 ctacatccgt ggcaatgcgc tggatctcga taactgggcg caagaacccg gtctggagaa
    63301 ctggagttat ctcgactgcc tgccctacta ccgcaaggcc gagactcgcg atgtgggcga
    63361 gaacgactac cacggtggcg atggcccggt gagcgtcacc acctccaaac ccggcgtcaa
    63421 tccgctgttt gaagcgatga ttgaagcggg cgtgcaggca ggctacccgc gcacggacga
    63481 tctcaacggc tatcagcagg aaggttttgg cccgatggat cgcaccgtca cgccgcaggg
    63541 ccgtcgcgcc agtaccgcgc gcggttatct cgatcaggcc aaatcgcgcc ctaacctgac
    63601 cattcgtact cacgctatga ccgatcacat catttttgac ggcaaacgcg cggtgggcgt
    63661 cgaatggctg gaaggcgaca gcaccatccc aacccgcgca acggccaaca aagaagtgct
    63721 gttatgtgca ggcgcgattg cctcaccgca gatcctgcaa cgctccggcg tcggcaacgc
    63781 tgaactgctg gcggagtttg atattccgct ggtgcatgaa ttacccggcg tcggcgaaaa
    63841 tcttcaggat catctggaga tgtatctgca atatgagtgc aaagaaccgg tttccctcta
    63901 ccctgccctg cagtggtgga accagccgaa aatcggtgcg gagtggctgt ttggcggcac
    63961 tggcgttggt gccagcaacc actttgaagc aggtggattt attcgcagcc gtgaggaatt
    64021 tgcgtggccg aatattcagt accatttcct gccagtagcg attaactata acggctcgaa
    64081 tgcagtgaaa gagcacggtt tccagtgcca cgtcggctca atgcgctcgc caagccgtgg
    64141 gcatgtgcgg attaaatccc gcgacccgca ccagcatccg gcgattctgt ttaactacat
    64201 gtcgcacgag caggactggc aggagttccg cgacgcaatt cgcatcaccc gcgagatcat
    64261 gcatcaaccg gcgctggatc agtatcgtgg ccgcgaaatc agccccggtg tcgaatgcca
    64321 gacggatgag cagctcgatg agttcgtgcg taaccacgcc gaaaccgcgt ttcatccgtg
    64381 cggtacctgc aaaatgggtt atgacgagat ggctgtggtt gacggcgaag gccgcgtaca
    64441 cgggctggaa ggcctgcgcg tggtggatgc gtcgattatg ccgcagatta tcaccgggaa
    64501 tttgaacgcc acgacaatta tgattggcga gaaaatagcg gatatgattc gtggacagga
    64561 agcgctgccg aggagcacgg cgggatattt tgtggcaaat gggatgccgg tgagagcgaa
    64621 aaaatgagtc gtgatgtgaa ctaacgcagg aaccgatcag attgagagtt accgttccag
    64681 agagggggac cgaatcctta tataaacact gaggtaactc tcatgcttca tatccagtat
    64741 tcatgatgct ggctttttgc tgatatttaa cccgaacatt cctgatacag actattaata
    64801 caaccttatc tgcgacagat tttattttct ggatatatta tgcgtgaaca aatcaaacag
    64861 gatatcgatc tgattgagat tttattttat ctgaagaaaa agattcgcgt tatccttttt
    64921 attatagcta tctgtatggc aatggtgctg ttttttctgt atatcaataa agataatata
    64981 aaagtgagtt acagcctaaa aataaaccag acaacaccag gcatacttgt taactgtgat
    65041 agcaataata actttgccgg tcagaccaca atgactgaag atgttataca gcgaattact
    65101 acattttttc acaccagccc agatgtcaag aacagagaaa taaagctgga atggtcagga
    65161 gataagagag atttaccaac tgctgaaacg gaaatatctc gcgtgcaggc gtctattatc
    65221 aaatggtatg cgtcagaata ccataatggc aggcaagttc tcgatgagat acaaacacct
    65281 tcagcaatta acagtgagct ttatacaaaa atgatatacc tgaccaggaa ctggtcattg
    65341 tatccgaacg gtgatggctg tgtaactata agctcaccag aaataaaaaa taaataccct
    65401 gctgccattt gcctggctct gggatttttt ctaagcattg taatttctgt aatgttttgc
    65461 cttgtcaaaa aaatggtaga tgaataccaa caaaactctg ggtagtaata aacagcattc
    65521 tgatagagag tgcctgtata aagctgaagt gacaatgacc gggaagggaa cccgttttca
    65581 ggaatggatg cctgtctttt tcactgtaaa tatcctggca ttactaatgc caggatattt
    65641 acagaagagc agcataacca tgacggaaca gggttaagtc aggttgtcct taacgctatt
    65701 ttcaacctcg tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc
    65761 tcaataatca gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca
    65821 atatccgccg ttgaactagg gccgctaatg atgttaatgc aggacggcat tcgttcaccg
    65881 gcctgcgctt tctgatgcaa tttttctgcg agttgcgcta cacgcggcaa gatagtgctt
    65941 ttacgcagga taaaaagaga agattccggg agcaggctca atgaacgccc gcgcccggcg
    66001 gcggaaaaaa gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc
    66061 actttagcct gctcggcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg
    66121 gcattgcatt cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg
    66181 ctaatcacga ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc
    66241 ttcgcctcgc tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac
    66301 gcgtcacagc gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc
    66361 ggcgcatctt ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta
    66421 ttcaaaaatt cgcctcgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca
    66481 actacggaaa ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat
    66541 cgcgccaaat ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc
    66601 ggccatcatc ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat
    66661 cgcccgttgc tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa
    66721 aatcagtttt gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga
    66781 gcaggcgtag ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc
    66841 accaattggc cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg
    66901 acaagtattc atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc
    66961 cagcacctca gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac
    67021 gtgccccgct tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac
    67081 ggcactgcgc gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg
    67141 ctccattccc atcactgcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc
    67201 ttcattggtc accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat
    67261 acctatttca gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc
    67321 ttcaggcgtt tccggcccct catagcccag acgttcatgt agcactcgac ggatctgatg
    67381 gcgatcttta tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag
    67441 aatatattca cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt
    67501 gacaccaatc tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg
    67561 ttgggcaacc tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg
    67621 accgccgttt tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag
    67681 aacatgatca cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc
    67741 gaccattttt tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat
    67801 gatcggatct tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat
    67861 cgacatcttt atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt
    67921 tctgcccttc ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa
    67981 ttaaatactc agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg
    68041 atatttcggc cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag
    68101 caaaggtcaa cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca
    68161 ctcccagctt acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg
    68221 cacctacatc gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg
    68281 cggcaacctt ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac
    68341 tttttacggc ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca
    68401 gtgcggcgat cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg
    68461 caggctgacc gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca
    68521 gtagcagcac ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa
    68581 agaaattgac attcacaact aatactccat tacttcatgc ccatttatgc ccgatgtttc
    68641 ccccccaccg ggggatcaat catcaataaa tatggcgttc ttttttaatt ggctagcaac
    68701 ataccacccc tcatttgtat gacaaatgat aatctttcat ccaaacttca aatgcaataa
    68761 ctaattttat ttctttcggc atcgctcaca ttttatgggg aaatcgacgt tcatttttgg
    68821 cataagaaat ccatttaaaa aggctacatc ccttatacct attcagagct acttattcag
    68881 gggt
//
LOCUS       DAZGXZ010000008        67779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000008 DAZGXZ010000000
VERSION     DAZGXZ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.008"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            204..1151
                     /gene="cyoA"
                     /locus_tag="ACHKJF_000626"
     CDS             204..1151
                     /gene="cyoA"
                     /locus_tag="ACHKJF_000626"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP0948044.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1173..3164
                     /gene="cyoB"
                     /locus_tag="ACHKJF_000627"
     CDS             1173..3164
                     /gene="cyoB"
                     /locus_tag="ACHKJF_000627"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP0948045.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3154..3768
                     /gene="cyoC"
                     /locus_tag="ACHKJF_000628"
     CDS             3154..3768
                     /gene="cyoC"
                     /locus_tag="ACHKJF_000628"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP0948046.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3768..4097
                     /gene="cyoD"
                     /locus_tag="ACHKJF_000629"
     CDS             3768..4097
                     /gene="cyoD"
                     /locus_tag="ACHKJF_000629"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP0948047.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4109..4999
                     /gene="cyoE"
                     /locus_tag="ACHKJF_000630"
     CDS             4109..4999
                     /gene="cyoE"
                     /locus_tag="ACHKJF_000630"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP0948048.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRVWARKLFGFSIVAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5184..5495
                     /locus_tag="ACHKJF_000631"
     CDS             5184..5495
                     /locus_tag="ACHKJF_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0948049.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            5479..6021
                     /locus_tag="ACHKJF_000632"
     CDS             5479..6021
                     /locus_tag="ACHKJF_000632"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0948050.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(6077..7012)
                     /locus_tag="ACHKJF_000633"
     CDS             complement(6077..7012)
                     /locus_tag="ACHKJF_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0948051.1"
                     /translation="MLTKRILFSLMLIVSPGVVASEKAHELYDSIYGGKPAPNVINTL
                     HKMAESGDADAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKHGDREAPLALGSIYYD
                     GEQVRVDYAKAYALFNQAAQYGVNLAWSRLGMMYANGQYVEVDCKKVKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDEGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            7420..8784
                     /gene="yajR"
                     /locus_tag="ACHKJF_000634"
     CDS             7420..8784
                     /gene="yajR"
                     /locus_tag="ACHKJF_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0948052.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRVEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(8912..9403)
                     /gene="yajQ"
                     /locus_tag="ACHKJF_000635"
     CDS             complement(8912..9403)
                     /gene="yajQ"
                     /locus_tag="ACHKJF_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP0948053.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            9571..10482
                     /gene="panE"
                     /locus_tag="ACHKJF_000636"
     CDS             9571..10482
                     /gene="panE"
                     /locus_tag="ACHKJF_000636"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP0948054.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            10445..11035
                     /gene="yajL"
                     /locus_tag="ACHKJF_000637"
     CDS             10445..11035
                     /gene="yajL"
                     /locus_tag="ACHKJF_000637"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP0948055.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(11089..12537)
                     /gene="thiI"
                     /locus_tag="ACHKJF_000638"
     CDS             complement(11089..12537)
                     /gene="thiI"
                     /locus_tag="ACHKJF_000638"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP0948056.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNDFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            12743..12985
                     /gene="xseB"
                     /locus_tag="ACHKJF_000639"
     CDS             12743..12985
                     /gene="xseB"
                     /locus_tag="ACHKJF_000639"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP0948057.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            12985..13884
                     /gene="ispA"
                     /locus_tag="ACHKJF_000640"
     CDS             12985..13884
                     /gene="ispA"
                     /locus_tag="ACHKJF_000640"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP0948058.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            13909..15771
                     /gene="dxs"
                     /locus_tag="ACHKJF_000641"
     CDS             13909..15771
                     /gene="dxs"
                     /locus_tag="ACHKJF_000641"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP0948059.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGFKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            15826..16800
                     /gene="yajO"
                     /locus_tag="ACHKJF_000642"
     CDS             15826..16800
                     /gene="yajO"
                     /locus_tag="ACHKJF_000642"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP0948060.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(16854..17372)
                     /gene="pgpA"
                     /locus_tag="ACHKJF_000643"
     CDS             complement(16854..17372)
                     /gene="pgpA"
                     /locus_tag="ACHKJF_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP0948061.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(17350..18327)
                     /gene="thiL"
                     /locus_tag="ACHKJF_000644"
     CDS             complement(17350..18327)
                     /gene="thiL"
                     /locus_tag="ACHKJF_000644"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP0948062.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLGLLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(18405..18824)
                     /gene="nusB"
                     /locus_tag="ACHKJF_000645"
     CDS             complement(18405..18824)
                     /gene="nusB"
                     /locus_tag="ACHKJF_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP0948063.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(18844..19314)
                     /gene="ribE"
                     /locus_tag="ACHKJF_000646"
     CDS             complement(18844..19314)
                     /gene="ribE"
                     /locus_tag="ACHKJF_000646"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP0948064.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(19403..20506)
                     /gene="ribD"
                     /locus_tag="ACHKJF_000647"
     CDS             complement(19403..20506)
                     /gene="ribD"
                     /locus_tag="ACHKJF_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP0948065.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQSGIDVSHGLMMSEVEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQSFRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(20510..20959)
                     /gene="nrdR"
                     /locus_tag="ACHKJF_000648"
     CDS             complement(20510..20959)
                     /gene="nrdR"
                     /locus_tag="ACHKJF_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP0948066.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            21110..21649
                     /gene="yajI"
                     /locus_tag="ACHKJF_000649"
     CDS             21110..21649
                     /gene="yajI"
                     /locus_tag="ACHKJF_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0948067.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESRIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     IPVEQLDFVRIHDIQPVMQ"
     gene            21948..22832
                     /gene="tsx"
                     /locus_tag="ACHKJF_000650"
     CDS             21948..22832
                     /gene="tsx"
                     /locus_tag="ACHKJF_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP0948068.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(23009..23356)
                     /gene="yajD"
                     /locus_tag="ACHKJF_000651"
     CDS             complement(23009..23356)
                     /gene="yajD"
                     /locus_tag="ACHKJF_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP0948069.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(23485..24456)
                     /gene="secF"
                     /locus_tag="ACHKJF_000652"
     CDS             complement(23485..24456)
                     /gene="secF"
                     /locus_tag="ACHKJF_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP0948070.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(24467..26314)
                     /gene="secD"
                     /locus_tag="ACHKJF_000653"
     CDS             complement(24467..26314)
                     /gene="secD"
                     /locus_tag="ACHKJF_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP0948071.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(26342..26674)
                     /gene="yajC"
                     /locus_tag="ACHKJF_000654"
     CDS             complement(26342..26674)
                     /gene="yajC"
                     /locus_tag="ACHKJF_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP0948072.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(26697..27824)
                     /gene="tgt"
                     /locus_tag="ACHKJF_000655"
     CDS             complement(26697..27824)
                     /gene="tgt"
                     /locus_tag="ACHKJF_000655"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP0948073.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(27880..28950)
                     /gene="queA"
                     /locus_tag="ACHKJF_000656"
     CDS             complement(27880..28950)
                     /gene="queA"
                     /locus_tag="ACHKJF_000656"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP0948074.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            29043..29624
                     /gene="acpH"
                     /locus_tag="ACHKJF_000657"
     CDS             29043..29624
                     /gene="acpH"
                     /locus_tag="ACHKJF_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP0948075.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(29629..31446)
                     /gene="malZ"
                     /locus_tag="ACHKJF_000658"
     CDS             complement(29629..31446)
                     /gene="malZ"
                     /locus_tag="ACHKJF_000658"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP0948076.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(31602..32975)
                     /gene="proY"
                     /locus_tag="ACHKJF_000659"
     CDS             complement(31602..32975)
                     /gene="proY"
                     /locus_tag="ACHKJF_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP0948077.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(33051..34370)
                     /gene="brnQ"
                     /locus_tag="ACHKJF_000660"
     CDS             complement(33051..34370)
                     /gene="brnQ"
                     /locus_tag="ACHKJF_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP0948078.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(34777..36072)
                     /gene="phoR"
                     /locus_tag="ACHKJF_000661"
     CDS             complement(34777..36072)
                     /gene="phoR"
                     /locus_tag="ACHKJF_000661"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP0948079.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDSGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAAEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(36130..36819)
                     /gene="phoB"
                     /locus_tag="ACHKJF_000662"
     CDS             complement(36130..36819)
                     /gene="phoB"
                     /locus_tag="ACHKJF_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP0948080.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            37009..38211
                     /gene="sbcD"
                     /locus_tag="ACHKJF_000663"
     CDS             37009..38211
                     /gene="sbcD"
                     /locus_tag="ACHKJF_000663"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP0948081.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            38208..41351
                     /gene="sbcC"
                     /locus_tag="ACHKJF_000664"
     CDS             38208..41351
                     /gene="sbcC"
                     /locus_tag="ACHKJF_000664"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP0948082.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            41477..42661
                     /gene="araJ"
                     /locus_tag="ACHKJF_000665"
     CDS             41477..42661
                     /gene="araJ"
                     /locus_tag="ACHKJF_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP0948083.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(42804..43712)
                     /gene="mak"
                     /locus_tag="ACHKJF_000666"
     CDS             complement(42804..43712)
                     /gene="mak"
                     /locus_tag="ACHKJF_000666"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP0948084.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            43837..44748
                     /gene="rdgC"
                     /locus_tag="ACHKJF_000667"
     CDS             43837..44748
                     /gene="rdgC"
                     /locus_tag="ACHKJF_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP0948085.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(44826..45245)
                     /locus_tag="ACHKJF_000668"
     CDS             complement(44826..45245)
                     /locus_tag="ACHKJF_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948086.1"
                     /translation="MYDLLTPTINLQIHCTRYPMRSQNGGSTDLPRYWITLDKNVIWD
                     YPKDFIAGNGGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAELLTKQFTSDKWGL
                     VNILRAADRRIGMRRLDQLQHKTHNIAALKIIARRSE"
     gene            complement(45449..45733)
                     /gene="ppnP"
                     /locus_tag="ACHKJF_000669"
     CDS             complement(45449..45733)
                     /gene="ppnP"
                     /locus_tag="ACHKJF_000669"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP0948087.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(45805..46482)
                     /gene="aroM"
                     /locus_tag="ACHKJF_000670"
     CDS             complement(45805..46482)
                     /gene="aroM"
                     /locus_tag="ACHKJF_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP0948088.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(46740..46931)
                     /gene="yaiA"
                     /locus_tag="ACHKJF_000671"
     CDS             complement(46740..46931)
                     /gene="yaiA"
                     /locus_tag="ACHKJF_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP0948089.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(46981..47505)
                     /gene="aroL"
                     /locus_tag="ACHKJF_000672"
     CDS             complement(46981..47505)
                     /gene="aroL"
                     /locus_tag="ACHKJF_000672"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP0948090.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(47688..48146)
                     /gene="yaiI"
                     /locus_tag="ACHKJF_000673"
     CDS             complement(47688..48146)
                     /gene="yaiI"
                     /locus_tag="ACHKJF_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP0948091.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            48266..49075
                     /gene="proC"
                     /locus_tag="ACHKJF_000674"
     CDS             48266..49075
                     /gene="proC"
                     /locus_tag="ACHKJF_000674"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP0948092.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(49092..50207)
                     /gene="adrA"
                     /locus_tag="ACHKJF_000675"
                     /gene_synonym="dgcC"
     CDS             complement(49092..50207)
                     /gene="adrA"
                     /locus_tag="ACHKJF_000675"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP0948093.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(50309..50629)
                     /gene="psiF"
                     /locus_tag="ACHKJF_000676"
     CDS             complement(50309..50629)
                     /gene="psiF"
                     /locus_tag="ACHKJF_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP0948094.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRITSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(50748..52163)
                     /gene="phoA"
                     /locus_tag="ACHKJF_000677"
     CDS             complement(50748..52163)
                     /gene="phoA"
                     /locus_tag="ACHKJF_000677"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP0948095.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(52264..52524)
                     /gene="iraP"
                     /locus_tag="ACHKJF_000678"
     CDS             complement(52264..52524)
                     /gene="iraP"
                     /locus_tag="ACHKJF_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP0948096.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(52706..52909)
                     /locus_tag="ACHKJF_000679"
     CDS             complement(52706..52909)
                     /locus_tag="ACHKJF_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948097.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYFWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            52987..54081
                     /gene="ddlA"
                     /locus_tag="ACHKJF_000680"
     CDS             52987..54081
                     /gene="ddlA"
                     /locus_tag="ACHKJF_000680"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0948098.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(54105..54317)
                     /gene="yaiZ"
                     /locus_tag="ACHKJF_000681"
     CDS             complement(54105..54317)
                     /gene="yaiZ"
                     /locus_tag="ACHKJF_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP0948099.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            54577..54885
                     /gene="yaiY"
                     /locus_tag="ACHKJF_000682"
     CDS             54577..54885
                     /gene="yaiY"
                     /locus_tag="ACHKJF_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP0948100.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(54944..56038)
                     /gene="yaiW"
                     /locus_tag="ACHKJF_000683"
     CDS             complement(54944..56038)
                     /gene="yaiW"
                     /locus_tag="ACHKJF_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP0948101.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAAKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(56051..57271)
                     /gene="sbmA"
                     /locus_tag="ACHKJF_000684"
     CDS             complement(56051..57271)
                     /gene="sbmA"
                     /locus_tag="ACHKJF_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP0948102.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            57623..58780
                     /gene="ampH"
                     /locus_tag="ACHKJF_000685"
     CDS             57623..58780
                     /gene="ampH"
                     /locus_tag="ACHKJF_000685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP0948103.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(58781..59404)
                     /gene="iprA"
                     /locus_tag="ACHKJF_000686"
     CDS             complement(58781..59404)
                     /gene="iprA"
                     /locus_tag="ACHKJF_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP0948104.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(59492..62470)
                     /gene="ehaB"
                     /locus_tag="ACHKJF_000687"
     CDS             complement(59492..62470)
                     /gene="ehaB"
                     /locus_tag="ACHKJF_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP0948105.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERANDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANS
                     LWPSSTVGVENSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINS
                     GSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGD
                     AALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLD
                     ADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVA
                     AATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
     gene            62996..63970
                     /gene="hemB"
                     /locus_tag="ACHKJF_000688"
     CDS             62996..63970
                     /gene="hemB"
                     /locus_tag="ACHKJF_000688"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP0948106.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(64076..64927)
                     /gene="tauD"
                     /locus_tag="ACHKJF_000689"
     CDS             complement(64076..64927)
                     /gene="tauD"
                     /locus_tag="ACHKJF_000689"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP0948107.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(64924..65751)
                     /gene="tauC"
                     /locus_tag="ACHKJF_000690"
     CDS             complement(64924..65751)
                     /gene="tauC"
                     /locus_tag="ACHKJF_000690"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP0948108.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(65748..66515)
                     /gene="tauB"
                     /locus_tag="ACHKJF_000691"
     CDS             complement(65748..66515)
                     /gene="tauB"
                     /locus_tag="ACHKJF_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP0948109.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(66528..67490)
                     /gene="tauA"
                     /locus_tag="ACHKJF_000692"
     CDS             complement(66528..67490)
                     /gene="tauA"
                     /locus_tag="ACHKJF_000692"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0948110.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT        16737 a        17860 c        16835 g        16347 t
ORIGIN      
        1 aaactccttc tgcactcccc tttcgttata acgccctttt gcaacagctt cttaaaatca
       61 acttgatatg ttttgcaaca tatgtgacct gacagccaaa tccaagtaac aggaatttaa
      121 tcatgtttac agtaatgtaa ccttcccgta aaatgccaac acactttaaa cgccaccaga
      181 tcccgtggaa ttgaggtcgt taaatgagac tcaggaaata caataaaagt ttgggatggt
      241 tgtcattatt tgcaggcact gtattgctca gtggctgtaa ttctgcgctg ttagatccca
      301 aaggacagat tggtctggag caacgttcac tgatactgac ggcatttggc ctgatgttga
      361 ttgtcgttat tcccgcaatc ttgatggctg ttggtttcgc ctggaagtat cgtgcgagca
      421 ataaagatgc taagtacagc ccgaactggt cacactccaa taaagtggaa gctgtggtct
      481 ggacggtacc tatcttaatc atcatcttcc ttgcggtact gacctggaaa accactcacg
      541 ctcttgagcc tagcaagccg ctggcacacg acgagaagcc cattaccatc gaagtggttt
      601 ccatggactg gaaatggttc ttcatctacc cggaacaggg cattgcaacc gtgaatgaaa
      661 tcgctttccc ggcgaacact ccggtgtact tcaaagtgac ctccaactcc gtgatgaact
      721 ccttcttcat tccgcgtctg ggtagccaga tttatgccat ggctggtatg cagactcgcc
      781 tgcatctgat cgccaacgaa cccggtactt atgacggtat ctccgccagc tacagcggcc
      841 cgggcttctc aggcatgaag ttcaaagcta ttgcaacacc ggatcgcgcc gaattcgacc
      901 aatgggtcgc aaaagcgaaa cagtcgccga acaccatgtc tgacatggct gcgttcgaaa
      961 aactggccgc gcctagcgaa tacaaccagg tggaatattt ctccaacgtg aaaccagact
     1021 tgtttgccga tgtgattaac aagtttatgg ctcacggtaa gagcatggac atgacccagc
     1081 cagaaggtga gcacagcgca cacgaaggta tggaaggcat ggacatgagc cacgcggaat
     1141 ccgcccatta aaggggttga ggaagaataa agatgttcgg aaaattatca cttgatgcag
     1201 tcccgttcca tgaacctatc gtcatggtta cgatcgctgg cattattttg ggaggtctgg
     1261 cgctcgttgg cctgatcact tacttcggta agtggaccta cctgtggaaa gagtggctga
     1321 cctccgtcga ccataaacgc ctcggtatca tgtatatcat cgtggcgatt gtgatgttgc
     1381 tgcgtggttt tgctgacgcc attatgatgc gtagccagca ggctcttgcc tcggcgggcg
     1441 aagcgggctt cctgccacct caccactacg atcagatctt caccgcgcac ggcgtgatta
     1501 tgatcttctt cgtggcgatg cctttcgtta tcggtctgat gaacctggtg gttccgctgc
     1561 agatcggcgc gcgtgacgtt gcgttcccgt tcctcaacaa cttaagcttc tggtttactg
     1621 ttgttggtgt gattctggtt aacgtttctc tcggcgtggg cgaatttgcg cagaccggct
     1681 ggctggccta tccaccgcta tcgggaatag agtacagtcc gggagtcggt gtcgattact
     1741 ggatatggag tctccagcta tccggtatag gtacgacgct taccggtatc aacttcttcg
     1801 ttaccattct gaagatgcgc gcaccgggca tgaccatgtt caagatgcca gtatttacct
     1861 gggcatcact gtgcgcgaac gtactgatta ttgcttcctt cccaattctg acggttaccg
     1921 tcgcgttgtt gaccctggat cgctatctgg gcacccattt ctttaccaac gatatgggtg
     1981 gcaacatgat gatgtacatc aacctgattt gggcctgggg ccacccagaa gtttacatcc
     2041 tgatcctgcc tgttttcggt gtgttctccg aaattgcggc aaccttctcg cgtaaacgtc
     2101 tgtttggtta tacctcgctg gtatgggcaa ccgtctgtat caccgtgctg tcgttcatcg
     2161 tttggctgca ccacttcttt acgatgggtg cgggcgcgaa cgtaaacgcc ttctttggta
     2221 tcaccacaat gattatcgcc atcccgaccg gggtgaagat cttcaactgg ctgttcacca
     2281 tgtatcaggg ccgcatcgtg ttccattctg cgatgctgtg gactatcggt tttatcgtca
     2341 ccttctcggt gggcggtatg actggcgtgc tgctggcagt accgggcgcg gacttcgttc
     2401 tacataacag cctgttcctg attgcgcact tccataacgt gatcatcggc ggcgtggtct
     2461 tcggctgctt cgcagggatg acctactggt ggcctaaagc gttcggtttc aaactgaacg
     2521 aaacctgggg taaacgcgcg ttctggttct ggatcatcgg cttcttcgtt gcctttatgc
     2581 cactgtacgc gctgggcttc atgggcatga cccgtcgttt gagccagcag attgacccgc
     2641 agttccacac catgctgatg attgcagcca gcggtgcagt actgattgcg ctaggtattc
     2701 tctgcctcgt tattcagatg tacgtttcta ttcgtgaccg cgaccagaac cgtgacctga
     2761 ctggcgaccc gtggggtggc cgtacgctgg agtgggcaac ctcttccccg cctccgttct
     2821 ataactttgc cgttgtgccg cacgttcacg agcgtgatgc attctgggaa atgaaagaga
     2881 aaggcgaagc gtataaaaag cctgaccact atgaagaaat tcatatgccg aaaaacagcg
     2941 gtgcaggtat cgtcattgca gctttctcca ccatcttcgg tttcgccatg atctggcata
     3001 tctggtggct ggcgattgtt ggcttcgcag gcatgatcat cacctggatc gtgaaaagct
     3061 tcgacgagga cgtggattac tacgtgccgg tggcagaaat cgaaaaactg gaaaaccagc
     3121 atttcgatga gattactaag gcagggctga aaaatggcaa ctgatacttt gacgcacgcg
     3181 actgcccacg cgcacgaaca cgggcaccac gatgcaggcg gaaccaaaat cttcggattt
     3241 tggatctacc tgatgagcga ctgcattctg ttctctatct tgtttgctac ctatgccgtt
     3301 ctggtgaacg gcaccgcagg cggcccgaca ggtaaggaca ttttcgaact gccgttcgtt
     3361 ctggttgaaa ctttcttgct gttgttcagc tccatcacct acggcatggc ggctatcgcc
     3421 atgtacaaaa acaacaaaag ccaggttatc tcctggctgg cgttgacctg gttgtttggt
     3481 gccggattta tcgggatgga aatctatgaa ttccatcacc tgattgttaa cggcatgggt
     3541 ccggatcgca gcggcttcct gtcagcgttc tttgcgctgg tcggcacgca cggtctgcac
     3601 gtcacttctg gtcttatctg gatggcggtg ctgatggtgc aaatcgcccg tcgcggcctg
     3661 accagcacta accgtacccg catcatgtgc ctgagcctgt tctggcactt cctggatgtg
     3721 gtttggatct gtgtgttcac tgttgtttat ctgatggggg cgatgtaatg agtcattcta
     3781 ccgatcacag cggcgcgtcc catggcagcg taaaaaccta catgacaggc tttatcctgt
     3841 cgatcattct gacggtgatt ccgttctgga tggtgatgac aggagctgcc tctccggccg
     3901 taattctggg aacaatcctg gcaatggcag tggtacaggt tctggtgcat ctggtgtgct
     3961 tcctgcacat gaataccaaa tcagatgaag gctggaacat gacggcgttt gtcttcaccg
     4021 tgctaatcat cgctatcctg gttgtaggct ccatctggat tatgtggaac ctcaactaca
     4081 acatgatgat gcactaagag cggcggttat gatgtttaag caatacctgc aagtaacgaa
     4141 accaggcatc atctttggca acctgatctc ggtgattggg ggattcctgc tggcctcaaa
     4201 gggcagcatt gattatcccc tgtttatcta cacgctggtt ggggtgtcac tggttgtggc
     4261 gtcgggttgt gtgtttaaca actacatcga cagggatatc gacagaaaga tggaaaggac
     4321 gaagaatcgg gtgctggtga aaggcctgat ctctcctgct gtctcgctgg tgtacgccac
     4381 gttgctgggt attgctggct ttatgctgct gtggtttggc gcgaatccgc tggcctgctg
     4441 gctgggggtg atgggctttg tggtttatgt cggcgtttat agcctgtaca tgaaacgcca
     4501 ctcggtctac ggcacgttga ttggttcgct ctccggcgct gcgccgccgg tgatcggcta
     4561 ctgtgcggta accggtgagt tcgatagcgg cgcagcgatc ctgctggcta tcttcagcct
     4621 gtggcagatg cctcactcct atgccatcgc cattttccgt tttaaggatt accaggcggc
     4681 aaacattccg gtattgccag tggtaaaagg catttcggtg gcgaagaatc acatcacgct
     4741 gtatatcatc gcctttgccg ttgccacgct gatgctctct cttggcggtt acgctgggta
     4801 taaatatctg gtggtcgccg cggcggttag cgtctggtgg ttaggtatgg ccctgcgcgg
     4861 ttataaagtt gctgatgaca gagtttgggc gcgcaagctg ttcggcttct cgatagtcgc
     4921 catcactgcc ctctcggtga tgatgtccgt tgattttatg gtgccggact cgcatacgct
     4981 gctggctgct gtttggtaac acaacctctc tattaaaaag gtgctacggc acctttttta
     5041 ttgtctgaga tcagagatac ggctaataac cgtatgtctt gacagagtga gcagatgaac
     5101 ataaattaat catacggttg atagccgtat tatgtatgag atcgactccc tacgatttca
     5161 ttcagttaat caaagaggtc ataatgcctg gaaaaacagc cactctaccc gatgtcgata
     5221 aaacgctgaa aaatgcgcgg gtcgaactca aaactagtcc cgacgccaaa aacaaacttc
     5281 gtgaagctgc gcaagccgtt ggggtagatt taagcgcttt tattttgagc gctgctatgg
     5341 aacgcgccga aagtgtgctt gataaccaac gtcgccgtga gctttcgaat caaagctggg
     5401 aactgatgaa ccaactcatc gctgaacctg ctcaaccgac gctcgccctc aaggcgttaa
     5461 tgaaaaggaa aaacagcgat ggtcgacaag catgaagaga ttactctgcc catagtcctc
     5521 tcctgtaatt atcagtctga tattacttat cctgggcaaa aacagtttga ttgcggtaac
     5581 cctgttatcg ataaatttgt acgcgcatcg ctaaagaaaa gtgtgcgtaa tagcgactgt
     5641 gcggctaaag cacttattga cagacaaagt ggtgaactga tcggcatctg tacttttacg
     5701 gcatattcgc tggaaaaaca acgcgtttct ggcgtccttc agggttcaca accttcagaa
     5761 attggtgttg tcagattagt catgttgggg gtagcacgga agtatcaaaa gcggggcttt
     5821 ggtcaggacc tactatgtga tttttttgaa catgtaaaaa taattcacca ggcattacca
     5881 attaaagggg tttatcttga tgctgaccct gccgccatta atttttatgc tcgtctcggc
     5941 tttgttcagc tttcagcgac accaaatgct tttggtgctg tacctatgtt tttggcgatt
     6001 cagcatattc tcgcggctta gattattatc gccacccgcc agggcaagtt ttgcctcagc
     6061 cgggggctga gcagcgttac cacttttgtt caattgcccc cgctttcagc aactccacat
     6121 ccttgccgtt aatggttgcc gttgcggcaa cgattgtggt ccttacagtt gcacaagatt
     6181 ttcgctcaaa ttccggttta taaagaataa gagacgaatt actggtaaat gaaccaaata
     6241 ggtaatcagt aaagctaata ttcagccggt ttaaaccgaa aggcgcgaag ccaaccgttt
     6301 ggttaatctc cttaccggat gggcgacgaa tgctgaatgt cacgcgaagg ttggccactt
     6361 cgcctaattt attggtggtg gcgaagaggc tatccatgca ggaaaaacct tcatctaaaa
     6421 aattatcccg catcccagaa cgggtaaccg tgatcacggg taacgtatcg tcaaccgttt
     6481 ttctgtcaat catgtctttt cgacaagtag ccagaaagtc ttccgggcca ccataaatgt
     6541 ggacaccttt atcaagatat tccttcactt tcttacaatc tacctcaaca tactgaccat
     6601 tggcgtacat catgcccaac ctggaccatg ctaaattcac accgtattgc gcggcctgat
     6661 taaatagcgc atacgctttg gcataatcca cccgcacctg ctcgccgtcg tagtagatac
     6721 tccccagcgc taacggagct tctctgtcac cgtgtttagc ggctaactcg aaccatttaa
     6781 tcgcttcctg aacatcaggt ttggtatcat agcgaggctg ataatattcc cagcctaaca
     6841 ggctctgcgc gtcggcatcc cccgattcag ccattttatg cagtgtattt ataacattgg
     6901 gcgcaggttt accaccgtat atgctgtcat acaattcatg tgctttctcg cttgccacca
     6961 cgcctggtga aactattagc attaaagaaa atagtattct tttagttaac atgtatacct
     7021 ttatgttctc tctgtcatat atttccataa taattaacca ccaaaaatcc atttaatgaa
     7081 taaatacagt gccataaagg cccaagtaat aagcacacat ttaacctgaa acattatcat
     7141 cgatgtcacc aaatcgttta tttagtgaga taaaaatagc atgttcgtaa gataaaaaat
     7201 gcacatagca atcaagaaat aatattatta cttttgatgt aaaactattt agctttaaga
     7261 ttcatttttt tcaacatata gagcattcat tttatgaatg caaaataaac atacctgaca
     7321 cacaaactat tctcctctgt aatattttct cattaacact ctgtttaccc aacatgcgct
     7381 ccgcactaca ctatgtgggc atttttttaa ggggtggtaa tgaacgatta taaaatgacg
     7441 ccaggtgaga ggcgcgcgac ctggggttta gggaccgtat tctcgttgcg catgctgggc
     7501 atgtttatgg ttctgccggt tctgaccacg tacggcatgg ctctgcaagg tgccagcgaa
     7561 gcattaatcg gtattgccat tggtatttat ggtctgactc aggccgtttt tcagattccg
     7621 tttggcctgc tttccgaccg cattggtcgc aaaccattaa ttgtcggtgg gctggcggtg
     7681 tttgccgccg gtagcgttat cgctgcgctt tccgactcta tctggggaat tattctgggc
     7741 cgggcgttac aaggctccgg tgcgattgcc gctgccgtta tggcgctgct ttccgatctc
     7801 acgcgcgaac aaaaccgcac caaagcaatg gcgtttatcg gcgtgagctt tggcattacc
     7861 tttgccattg cgatggtgct tggcccgatc atcactcaca aacttgggct gcacgcgctg
     7921 ttctggatga tcgctattct ggcaacgacc ggcattgcat tgaccatttg ggttgtgccc
     7981 aacagtagca ctcacgtact taatcgtgag tccggaatgg tgaaaggcag tttcagtaaa
     8041 gtgctggcgg aaccgcggct actgaaactc aactttggca ttatgtgtct gcatattttg
     8101 ctgatgtcga cgtttgttgc cctgcccgga cagctggctg atgcggggtt cccggcggct
     8161 gaacactgga aggtctacct ggcaacgatg ctaatcgcct ttggctcggt cgtgcctttc
     8221 attatctacg ctgaagttaa gcgcaaaatg aagcaagtct ttgtcttctg cgtcgggttg
     8281 atcgtagttg cggaaattgt gttgtggaac gcacaaacgc agttctggca acttgtggtc
     8341 ggcgtgcagc ttttctttgt ggcgtttaat ttgatggaag cccttctgcc ctcacttatc
     8401 agtaaagagt cgccagcagg ttacaaaggt acggcgatgg gtgtctactc caccagccag
     8461 tttcttggcg tggcgattgg cggttcgctg ggcggctgga ttgacggcat gtttgacggt
     8521 cagggggtat ttctcgctgg cgcaatgctg gccgcagtgt ggctggcagt cgccagtacc
     8581 atgaaagaac cgccgtatgt cagcagcttg cgcgttgaaa tcccagcgga cattgccgca
     8641 aacgaagcgt taaaagtacg tttgctggaa actgaaggcg tcaaagaagt gttgattgca
     8701 gaagaagaac attcagctta tgtgaaaatc gacagcaaag tgacgaatcg ctttgaggtt
     8761 gaacaggcaa ttcgtcaggc ataaaaaacg aggcgaattg cctgctgcgc ttagcttacc
     8821 aggcctacaa agtttctgaa ttacaaacct ttgtaggccg aatcaggcgt tcatcccgca
     8881 tctgacatga acaaaacgca catagtcgcg attaatcgcg gaagtttttg aactggaacg
     8941 gctgaccgag atcgccaccg cgtaccatcg ccatgacagc ctgcaaatca tcacgagatt
     9001 tgcccgttac gcggatttca tcgccctgaa tttgcgcctg cactttcagt ttgctgtctt
     9061 tgatcatctt gacgattttc ttctgggtcg cgctctcaat gccctgtttc agtttcgctt
     9121 ccacaaacca ggttttaccg ctatgaacga tattctccgg tacatccagc gaactgcctt
     9181 caatgccgcg cttcagcagc ttggcacgca gaatatccag caactgattg acctggaaat
     9241 cggactcgct caacactttg atggttttgc tggcgtcgtt cagctcaaat gaggcttcaa
     9301 cattacggaa gtcaaaacgg gactccactt cgcggctcgc gttatcgacc gcgttacgtg
     9361 cttcctgaag atcaacttca gagacaatat cgaaagatgg catcttttcc tctcccttca
     9421 tttttgatgc gaagcataat acccgcaaag ttaaataacc gacagtcctg caccaggacg
     9481 tatcccttaa ttgtgcatag tcgcaacagc atgcacaatt aagggatagc cttattgaac
     9541 acccggagtg gttgcgggtg aggaggaaca atgaaaatta ccgtattggg atgcggtgcc
     9601 ttagggcaat tatggcttac agcactttgt aaacagggtc acgacgttca gggctggctg
     9661 cgcgtaccgc aaccttattg tagtgtgaat ctggttgaga cagatggttc gatatttaac
     9721 gaatcgctga ccgctaacga tcccgatttt ctcgccacca gcgatctgct cctggtgacg
     9781 ctgaaagcat ggcaggtttc cgatgccgtc aaaagcctcg cgtccacact gcctgtaacc
     9841 acgccaatac tgttaattca caacggcatg ggcaccatcg aagagttgca aaacattcag
     9901 cagccattac tgatgggcac caccacccat gccgcccgcc gcgacggcaa tgtcattatt
     9961 catgtggcaa acggtatcac gcatattggc ccggcacggc aacaggacgg cgattacagt
    10021 tatctggcgg atattttgca aaccgtgttg cctgacgtcg cgtggcataa caatattcga
    10081 gccgagttgt ggcgcaagct ggcagtcaac tgcgtgatta atccactgac cgccatctgg
    10141 aattgcccga acggtgaatt acgtcatcat ccgcaagaaa ttatgcaaat atgcgaagaa
    10201 gtcgcggcag tgatcgaacg cgaagggcat catacttcag cggaagattt gcgtgattac
    10261 gtgatgcagg tgattgatgc aacagcggaa aatatctcgt cgatgttgca ggatatccgc
    10321 gcgctgcgcc acaccgaaat cgactatatc aatggttttc tcttacgccg cgcccgcgcg
    10381 catgggattg ccgtaccgga aaacacccgc ctgtttgaaa tggtaaaaag aaaggagagt
    10441 gaatatgagc gcatcggcac tggtttgcct cgcccctggt agtgaagaga ctgaagccgt
    10501 caccacgatc gacctcttgg ttcgcggcgg tatcaaagtc accactgcca gcgtagccag
    10561 cgatggcaac ctggcaatta cctgctcgcg cggcgtgaag ctgctggcgg atgcgccgct
    10621 ggtcgaagtg gctgatggcg aatatgacgt gatcgtgctg cctggtggca ttaaaggcgc
    10681 ggagtgtttt cgcgatagca ccctgctggt tgaaaccgtt aaacagttcc accgttccgg
    10741 gcgtatcgtc gcggctattt gcgccgcgcc agccaccgtg ctggtgccgc acgatatctt
    10801 cccgattggt aatatgaccg gcttcccgac gctgaaagac aaaattcccg ccgaacaatg
    10861 gcaggacaag cgcgtcgtct gggatgcacg ggtaaaattg ctgaccagcc aggggccggg
    10921 tacggctatt gattttggtc tgaaaattat cgacctgctg gttgggcgcg agaaagccca
    10981 tgaagtggca tcacaactgg tgatggcggc agggatttat aattattacg agtagtgtcg
    11041 gatgcggcaa acgtcgcatc tgaccagatg cgacgtaaaa accacaaatt acgggcgata
    11101 caccttcaca ttattaaagc cctgctcgcg cagatagagc gcctgcaggc ggctcatcac
    11161 cccacgctca caccacaata gccaggtttt gctctggtcg agatcgccaa atttggtgct
    11221 cagtttatag aacggcagag aaaccacatc aatcccttcg actttcagtg gcttatcttc
    11281 ctgttcatcg acagaacgga tatcgagaat cacgtcgttc gggccgaagt cattgacggt
    11341 ttccacttcc accacttcct gctcggtctg ctgggcgatt tcgcggatat caacgttatt
    11401 cgcttcttca accactttat cgagaatgct gaagtcgaac ttctcttctt ccgcttcaat
    11461 cttcgattta accgctttca ccgtcgggct tttggagatc acgccacaat attccggcat
    11521 cgtgcgagca aagtcttcgg tgccaatctg gcgagccagg ttgatgatgt gctctttgtc
    11581 gtaagagatc agcggacgca ggatcaacgt atcggagaca ttatcaatta aacgcagatt
    11641 ggtcagggtc tggctggaca cctggcccag tgcttcgccg gtgaccagcg cctgtacgcc
    11701 gtaacgttca gccactttag acgcggcacg caccatcata cgtttgagga taacgcccat
    11761 ctgaccgtcg tcgattttct cgagaatttc cccgacgacc ggctcgaaat taatagcgac
    11821 aaaacgcacg cggtgggagc tgccaaagcg gttccacaga taatgtgcca cctgacgcac
    11881 gccaatttca tgcgccgcgc cgccaaggtt aaagaagcag taatgcacgc ggcagccgcg
    11941 acgcatcaac atataactgg aaacaccgga gtcgaaacca ccggaaatga gcgacagcac
    12001 atcttcctgg gtgccgatcg ggaaaccgcc aataccttcg tagcggcctt taatcagcag
    12061 gagacgatcg tcttccactt ccagatggac agtcacttcc ggattggtca gcttcacgcg
    12121 cgcggattca atatgctgat ttaaaccgcc gccgacgtaa cgttccacat caatcgagct
    12181 aaaatcatgt ttgccacggc gcttcacgcg tacgcagaag gttttgcctt ccagctgatc
    12241 gcgatactga accaacgctt tctcgaaaat atcgtgcatg tcggtaaacg gcacgtcttc
    12301 gacttcgaga atatggtgga tacccggaat acgggtcaga gcgtcgcgaa tagccagacg
    12361 ctggttttca tctttcgcgc gaacttcgat gttatcccag tggcggacga cagcgagcgt
    12421 ctcatcatag tgctttaaaa cgttacgaat gttcccggta aggattttta taaagcgcaa
    12481 gcgcacagat tggcttttga tggtgatttc cgggaacaat ttaatgataa acttcatggc
    12541 ggcaatggtt cgttggcaag ccttaagcga cttgtatagg gaaaaataca gcagcccaca
    12601 cctgcggctg catccaggcg cggaagtata ccactaacat cgctttgctg cgcacatcac
    12661 cttaccattg cgcgttattt gctatttgcc ctgagtccgt taccatgacg gggcgaaaaa
    12721 tattgagagt cagacattca ttatgccgaa gaaaaatgag gcgcccgcca gctttgaaaa
    12781 ggcgctgagc gagctggaac agattgtaac ccgtctggaa agtggcgacc tgccgctgga
    12841 agaggcgctg aacgagttcg aacgcggcgt gcagctggca cgtcaggggc aggccaaatt
    12901 acaacaagcc gaacagcgcg tacaaattct gctgtctgac aatgaagacg cctctctaac
    12961 cccttttaca ccggacaatg agtaatggac tttccgcagc aactcgaagc ctgcgttaag
    13021 caggccaacc aggcgctgag ccgttttatc gccccactgc cctttcagaa cactcccgtg
    13081 gtcgaaacca tgcagtatgg cgcattatta ggtggtaagc gcctgcgacc tttcctggtt
    13141 tatgccaccg gtcatatgtt cggcgttagc acaaacacgc tggacgcacc cgctgccgcc
    13201 gtagagtgta tccacgctta ctcattaatt catgatgatt taccggcaat ggatgatgac
    13261 gatctgcgtc gcggtttgcc aacctgccat gtgaagtttg gcgaagcaaa cgcgattctc
    13321 gctggtgacg ctttacaaac gctggcgttc tcgattttaa gcgatgccga tatgccggaa
    13381 gtgtcggacc gcgacagaat ttcgatgatt tctgaactgg cgagcgccag tggtattgcc
    13441 ggaatgtgcg gtggtcaggc attagattta gacgcggaag gcaaacacgt acctctggac
    13501 gcgcttgagc gtattcatcg tcataaaacc ggcgcattga ttcgcgccgc cgttcgcctt
    13561 ggtgcattaa gcgccggaga taaaggacgt cgtgctctgc cagtactcga caagtacgca
    13621 gagagcatcg gccttgcctt ccaggttcag gatgacatcc tggatgtggt aggagatact
    13681 gcaacgttgg gaaaacgcca gggtgccgac cagcaacttg gtaaaagtac ctaccctgca
    13741 cttctgggtc ttgagcaagc ccggaagaaa gcccgggatc tgatcgacga tgcccgtcag
    13801 tcgctgaaac aactggctga acagtcactc gatacctcgg cactggaagc gctagcggac
    13861 tacatcatcc agcgtaataa ataaacaata agtattaata ggcccctgat gagttttgat
    13921 attgccaaat acccgaccct ggcactggtc gactccaccc aggagttacg actgttgccg
    13981 aaagagagtt taccgaaact ctgcgacgaa ctgcgccgct atttactcga cagcgtgagc
    14041 cgttccagcg ggcacttcgc ctccgggctg ggcacggtcg agctgaccgt ggcgctgcac
    14101 tacgtctata acactccgtt tgaccaattg atttgggatg tggggcatca ggcttatccg
    14161 cataaaattt tgaccggacg ccgcgacaaa atcggcacca tccgtcagaa aggtggtctg
    14221 cacccgttcc cgtggcgcgg cgaaagcgaa tatgacgtat taagcgtcgg gcattcatca
    14281 acctccatca gtgccggaat tggtattgcg gttgctgccg agaaagaagg caaaaatcgc
    14341 cgcaccgtct gtgtcattgg cgatggcgcg attaccgctg gcatggcgtt tgaagcgatg
    14401 aatcacgcgg gcgatatccg tcctgatatg ctggtggtcc tcaacgacaa tgaaatgtcg
    14461 atttccgaaa atgtcggcgc gctcaataac catctggcac agctgctttc cggtaagctt
    14521 tactcttcgc tgcgcgaagg cgggaaaaaa gttttctctg gcgttccgcc cattaaagag
    14581 ctgctcaaac gtaccgaaga acatattaaa ggcatggtag tgcctggcac gttgtttgaa
    14641 gagctgggct ttaactacat cggcccggtt gacggtcacg atgtgctggg gcttatcacc
    14701 acgctgaaga acatgcgcga cctgaaaggc ccgcagttcc tgcatatcat gaccaaaaaa
    14761 ggtcgtggtt atgaaccggc agaaaaagac cccatcactt tccacgccgt gcctaaattt
    14821 gatccctcca gcggttgttt gccgaaaagt agcggcggtt tgccgagcta ttcaaaaatc
    14881 tttggcgact ggttgtgcga aacggcagcg aaagacaaca agctgatggc gattactccg
    14941 gcgatgcgtg aaggttccgg catggtcgag ttttcacgta aattcccgga tcgttacttc
    15001 gacgtggcaa tcgccgagca acacgcggtg acctttgccg ccggtctggc gattggtggg
    15061 ttcaaaccca ttgtcgcgat ttactccact ttcctgcaac gcgcctatga tcaggtgctg
    15121 catgacgtgg cgattcaaaa gctcccggtc ctgttcgcca tcgaccgcgc gggcattgtt
    15181 ggtgctgacg gtcaaaccca tcagggcgct tttgacctct cttacctgcg ctgcataccg
    15241 gaaatggtca ttatgacccc gagcgatgaa aacgaatgtc gccagatgct ctataccggc
    15301 tatcactata acgacggccc gtccgcggtg cgctacccgc gcggtaacgc ggttggcgtg
    15361 gaactgacgc cgctggaaaa actgccaatt ggcaaaggca ttgtgaagcg tcgtggcgag
    15421 aaactggcga tccttaactt tggtacgctg atgccagacg cggcgaaagt cgctgaatcg
    15481 ctgaacgcta cgctggtcga tatgcgtttt gtgaaaccgc ttgatgaagc gttaattctg
    15541 gaaatggccg ccagccatga agcgctggtc accgtagaag aaaacgccat tatgggcggc
    15601 gcaggcagcg gcgtgaacga agtgctaatg gcccatcgta aaccagtacc cgtgctgaac
    15661 attggcctgc ctgacttctt tattccacaa ggaactcagg aagaaatgcg cgccgaactc
    15721 ggcctcgatg ccgccggtat ggaagccaaa atcaaggcct ggctggcata atccctactc
    15781 cactcctgct atgcttaaga aattattcat ggcaggagtg gcagcatgca atacaacccc
    15841 ttaggaaaaa ccgaccttcg cgtttcccga ctttgcctcg gctgtatgac ctttggcgag
    15901 ccagatcgtg gtaatcacgc atggacactg ccggaagaga gtagccgtcc catcattaaa
    15961 cgcgcgctgg aaggcggcat aaatttcttt gataccgcca atagctattc cgatggcagc
    16021 agcgaagaga tcgttggtcg cgcactgcgg gatttcgccc gtcgtgaaga cgtggtcgtt
    16081 gcgacgaaag tgttccatcg cgttggtgat ttaccggaag gattatcccg tgcacaaatt
    16141 ttgcgctcta tcgacgacag cctgcgccgt ctcggcatgg attatgtcga tatcctgcaa
    16201 attcatcgct gggattacaa cacgccgatc gaagagacgc tggaagccct gaacgacgtg
    16261 gtaaaagccg ggaaagcgcg ttatatcggc gcgtcatcca tgcacgcttc gcagtttgct
    16321 caggccctga agctacaaaa acagcacggc tgggcgcagt ttatcagtat gcaggatcac
    16381 tacaatctga tttatcgcga agaagagcgc gagatgctgc ctctgtgtta tcaggagggc
    16441 gtggcggtga ttccgtggag cccgctggcg cgggggcgac tgacgcgtcc gtggggagaa
    16501 actaccgcac gactggtgtc tgatgaggtg gggaaaaatc tctataaaga aagcgatgaa
    16561 aatgacgcgc agatcgcaga gcggttaacg ggcgtcagtg aagaactcgg tgcaacacga
    16621 gcacaagttg cgctggcctg gttgttgagt aaaccgggca ttgccgcacc gattatcggt
    16681 acatcgcggg aagaacagct tgatgagctg ctgaacgcgg tggatatcac tttgaagcca
    16741 gaacagattg ccgaactgga aacgccgtat aaaccgcatg cggtcgtagg atttaaataa
    16801 gagtccaggc ctgatgagac gtgacaagcg tcacatcagg catcggtgca caactacgac
    16861 agaataccca gcggccagtg atgaccgata aagtacagga tgcctgcgga aatcaccccg
    16921 gcgacaatat cgtcgatcat gatccccatg ccgccatgta cattgcgatc aaaccagcgg
    16981 atcggccacg gcttccacat atccagaata cggaaaatca caaacccggc ggcaacccac
    17041 tgccagtcat tggtcggcag cgccatgagc gtgatccaca taccaataaa ttcgtcccag
    17101 acaatgctgc cgtgatcgtg cacgcccata tctttcgccg tttgatgaca aagatagacg
    17161 ccgatacaga tccccaacat caccaccagc gagtagagct gccagggtaa aaaggtcatc
    17221 agataccaga acggaatcgc tgccagcgat cccatcgtgc caggaacgat tgggcttaat
    17281 ccacttccga atccgacagc aagcagatgc cacggattac tcatcttcag gcgacttttc
    17341 gcgacatctt tatggcgtgg caaaatggtc atatcctttc cagtcaaacg tgacaggttc
    17401 gccgtcacga ataaaacaaa gcccttcgat atcggcggtc atttgcccga tacaggtaaa
    17461 cggtacgcca aggtgtccga gagcaacatc cagcgcgcca cggttcagtt ccggcaccgt
    17521 gaaacacaac tcgtaatctt caccgccaga gagcgcccag cgcagcgcct gttccggttc
    17581 aacatggcga gaaagcgcat cagaaaacgg cagcaatccc aggtcaatac gtgcgccgca
    17641 gtcgctggct ttcacgatat gcccgagatc ggaaatcaga ccgtcagaga gatcgatagc
    17701 tgaatttgcc agatcgcgca gtgcctgccc ctgtaaaata cgcggcgatg gacggagatg
    17761 acgtttgatc aagtagtcag catctttagc atcggcaacc tgcaaacggt tttgcaaaat
    17821 cgccagcccg gcggcgctat cgcccggtgt accggtcaca tagatccaat cacccggttt
    17881 cgccccagag cgcgttaagg ctcgtcccat cggaacaaag ccgtggatac ccaacgtcat
    17941 tgataatggc ccacgcgtgg tatcgccgcc aatgagttgc atatcgtaat aattgagaag
    18001 atcaaacaaa ctgtcgctga aggactcaag ccacgcttcg tctacgtccg gtaaggttaa
    18061 tgccagcgtc agccaggccg gatcggcccc catcgctgcc agatcgctta ggttcaccgc
    18121 cagtgcttta taagccagat cagcaggatc gatatcaggg aggaaatggt tacccgccac
    18181 cagcgtatca gtgctgatcg ccagggtctg tttctcgggg atattgagaa gtgcgcaatc
    18241 gtcgccgatg cccagttcga catcaagacg agaacttctt acacggtcaa aataacgggc
    18301 aatcagggag aactcgccac atgccatacg ttatgcctca gcaggtaaaa aagaaaaggc
    18361 cggcgatcgc ggaacggtct tccgtgaatc taccggcctg gatatcactt tttgttaggg
    18421 cgaatcacag gtgctgcttt atcgagtacg ccgttgacga acttatggct gtcttctgcg
    18481 ccgaacgatt tcgccagttc gatcgcttcg ttaatggcca ctttgtatgg cacatcgcta
    18541 cgtttagaca gctcgtacag cgcaatgcgc agtactgctt tttctacctg gcccagttct
    18601 tccagcaggc gggacaggta tggcttcatc agtccgtcga ggtatgcggt gttagtcgcc
    18661 accccggcca gcagctcacg gaagtacagg acgtcaacgt cttttacatc ctgttcagcc
    18721 aggaactggt attcaacatc agcgatgtcg ttctgggaca actgccagga gtagagcgcc
    18781 tggacggcac actcacgagc gcggcgacga gcagcaggtt tcacggattt ccccttacta
    18841 atttcaggcc ttgatggctt tcaatacatt aatcatttca agcgcggtca gtgcagcttc
    18901 tgcacctttg ttgccagctt tggtgccagc acgttcgatc gcttgttcaa tgctttcagt
    18961 ggtcagaacc ccaaaagcaa ccggaatttc gctgtcctgg gcaacatgcg ccaggccgtt
    19021 gcttgcacca ccagcgacat attcaaagtg ggcagtgcca ccacgaataa ccgtacccag
    19081 cgcaatcacc gcgtcgtatt taccggtttt agccagtgca cccgccgcca gcggcagctc
    19141 ataggcacca ggcacccaaa caacggtaat gttttcatct tttacctgac cgatacgttt
    19201 cagtgcgtca attgcacctt ccagcaggct gtcattgata aagttgttga aacgcgcgat
    19261 ggtgatggcg acgcgagcgt ccggggtagc aacgttagct tcaataatgt tcattttctt
    19321 ccttcgggtt cgagtatggc cccgcagggg ggcggatttt agcataatat ttcgtgcgct
    19381 gcttcccttt cgagccggga gatcatgcac ccactaaatg caggcaaaca tccgggccta
    19441 catgacgtat ctctttgaat ttaaattggg gggcgtcggc taatttctca agccctggca
    19501 gcgagcataa tccacgggcg tcgctgccta atagtttagg tgcgatatag acaatcagct
    19561 catcgactaa acccacctgc agcaatgcgc cagcgagcgt tggccccgct tccacccaga
    19621 tgctgttaat ttgctgttta cccagttgca tcatcagtac aaccagatcc agatgacctt
    19681 tatgctctgg aatcagcaag gtacgcaccg tttccggcca ctcacgagaa tcttcctgcg
    19741 tacgcgcgaa ccaggtttcg ccgggctgct gcacaatgcg atgttccggc gtcacgcgat
    19801 tttggctatc aatcacaata cgtaccggct gacggaaact ttgttgcggg tagagcgcct
    19861 gagtttgttc atccagttcg gaccaacgca ccgttaaggc aggatcatcc gccagcaccg
    19921 tggcgctgct ggttaaaatg gcatgacttt gcgcgcgcag tcgttgtaca tcacgccgcg
    19981 cctggggcga agtgatccac tggctttcgc cgctcgccat cgccgtgcga ccatcaagcg
    20041 atgcgccaag ttttaactga atataaggaa agccggtgcg catccgcttg agaaagcctt
    20101 tattcaattg ctcgacttca ctcatcatca gcccgtggct gacgtcaatg ccagactgtt
    20161 gcagacggta aagtccacgc ccagcgacct gcgggttagg atcttgcatc gctgcgacca
    20221 cgcgcgctac gccagcggcg atgagtgcgt cacagcacgg tggcgtacga ccatgatggc
    20281 tacagggttc gagtgtgaca taggcggtcg cacctttggc tttttcaccc gccatacgca
    20341 acgcgtgtac ttcggcatgt ggttcacccg ctcgttggtg gtaaccttca ccgacaattt
    20401 cgccatcttt gacaatgacg cacccgacat tcgggttggg gtgggtggta aaacgtcctc
    20461 gttgcgccag ctttagcgcc cgcgccatgt aatactcgtc ctgcacggct tagtcctcca
    20521 ggcgcgcgat ctcttcgcca aattctttga tatcttcgaa actgcggtag acagaggcaa
    20581 aacggatata ggcgacttta tcgagctttt tcaattgctc catcaccaga ttgccaatca
    20641 tcttgctcgg tacttcgcgc tcaccggtgg cgcgcagctg cgatttaata tgattgattg
    20701 ccatttcaac gtcatcggaa ctcaccggac gtttttccag cgcccgcagc attccgctgc
    20761 gcaatttctc ttcattaaac ggttcacgca cgtcgttgct ttttacaaca cgcggcataa
    20821 ccagctccgc cacttcaaag gtggtgaaac gttcattaca caccagacac tgccgacggc
    20881 ggcgtacgga tgaaccctcg cccacgagac gagagtcaat taccttagtg tccacggcga
    20941 aacagaatgg gcaatgcata cggcgtcctg accaggtggt taacggaaat ctattttacc
    21001 ctgaactgac gatacaacaa aggcgcaacg cttttgagat aaaggatcga tgctttcgct
    21061 gcttttaccc tctaccatta ggctaattgc gacgagaaag ggacgaccta tgaatactaa
    21121 cgtttttcga ctgctcctgc tgggaagcct gttcagcctt agcgcctgtg tgcagcaaag
    21181 cgaagtgcga cagatgaaac acagcgtcag cacgctgaac caggagatga cgcaactcaa
    21241 tcaagaaaca gtcaaaatca ctcagcaaaa caggctgaat gcaaaatcca gcagtggggt
    21301 ttaccttctg cctggagcga aaacaccggc aagactggaa agccggattg gtactttacg
    21361 tatgtcgctg gtgaatatta cgcctgatac agatggcacc accctgacgc tacgtattca
    21421 gggcgagtcg aatgatccct tgcccgcatt cagcggaacc gttgaatatg ggcaaattca
    21481 gggaacaata gacaactttc aggaaatcaa tgtgcagaat caattgatta acgcccctgc
    21541 cagcgtcctc gcccccagcg atgttgatat tccgttacag ttaaagggga ttcctgtaga
    21601 acagttagac tttgtacgta tccacgacat tcaacctgtt atgcagtaaa cgtatttcgg
    21661 ggcgattttg tgcgtctcgc aacatctttc cccgtcattt tgttactccg cttacatcac
    21721 cctgattgat agtaaaagtt tgcaacaagg gtgaaagtca gtacaatccc tgcccgaatg
    21781 tgtgtaaacg tgaacgcaat cgattacgta aatgatagaa ctgtgaaacg aaacatattt
    21841 ttgtgagcaa tgatttttat aataggctcc tctgtatacg aaatatttag aaacgcaatt
    21901 tgcgcctttt tcactcccgc aagggatttt caaacagtgg catacatatg aaaaaaacat
    21961 tactggcagc cggtgcggta ctggcgctct cttcgtcttt tactgtcaac gcagctgaaa
    22021 acgacaaacc gcagtatctt tccgactggt ggcaccagag cgttaacgtt gtcggaagct
    22081 atcacacccg tttcggaccg cagatccgca acgataccta ccttgagtac gaagcattcg
    22141 ctaaaaaaga ctggttcgac ttctatggtt atgcggatgc gccggtattc ttcggcggta
    22201 actccgatgc taaaggtatc tggaaccacg gttctccgct gtttatggaa atcgaaccac
    22261 gtttctccat cgacaagctg accaatactg accttagctt cggtccgttc aaagagtggt
    22321 acttcgcgaa caactacatt tacgacatgg gtcgtaataa agatggtcgc cagagcacct
    22381 ggtacatggg tctgggtacc gacatcgata ctggtctgcc gatgagcctg tccatgaacg
    22441 tctatgcgaa ataccagtgg cagaactatg gcgcagcgaa cgaaaacgag tgggacggtt
    22501 accgtttcaa aattaaatac tttgtgccga ttaccgatct gtggggcggt cagctgagct
    22561 acatcggctt caccaacttc gactggggtt ccgatttagg ggatgacagc ggtaacgcaa
    22621 tcaacggtat taagacccgt actaataact ctatcgcttc cagccatatt ctggctctga
    22681 actacgatca ctggcactac tctgtcgtag ctcgttactg gcacgacggt ggtcagtgga
    22741 acgacgatgc agaactgaac ttcggcaacg gcaacttcaa cgttcgctct accggctggg
    22801 gtggttacct ggtagtaggt tacaacttct gattatgaaa atgccgggat ttattcccgg
    22861 catttcagat tgttaacaac gtgcgctttg ttcatgccgg atgcggcgta aacgctttgt
    22921 ccgacccaca aaagcacgta aattcaatat attgctgaaa taacgtagtc ctgataagcg
    22981 tagcgcatca ggcaatttta cgttttaatc acttcttctt gttcatcatc gctttcaggt
    23041 cagcgaatgg gttgtacttc gcttcaccga catctttctg cgcatcttcc cctgcgataa
    23101 cggtcgtacc atactgatcc gcttcggtat atttcgaatg ctcatgatcg tggcaataga
    23161 gacaaagcaa ttcccagtta ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt
    23221 ggtgaacagt aagttcacgc aggttggaat aaacaaactc gcgggaacag cgaccgcaga
    23281 cccacggata tatttttaat gctttttcgc gatagccgct ttctaaccgc gcatagtttt
    23341 ttgggatgat agccatgaag agttacctgg atgaaatgta gggttacgcg cagtttccca
    23401 agatgcgggg attttagcaa tctccataca tcaaaaaaaa tcccgatctt ctgaccggga
    23461 ttttcaacat caacgggaac ttgattacgg cagaattgac ggctgatccg ccccttcttt
    23521 ttccactttc tgctgcaaca tgtgttcgcg cttcataccc agtttcagag ccaacgcaga
    23581 tgccacatag atggaagatg cagtaccgat ggaaacaccg ataagcatgg tcagcgagaa
    23641 gccttccagt accggaccac cgaagaggta cagcatcagg ataaccatca aggtagtacc
    23701 ggatgtgatc aaggtacggt gcagcgtctg ggtcaaggac acgttaaaga tttcgtaagg
    23761 cgtaccgcga cggatcttgc ggaagttttc acgaatacgg tccgatacca cgatactgtc
    23821 gttaagcgag taaccgataa ccgacatcaa cgatgccaca atggtcaggt caatctcgat
    23881 atggaataac gacaaaatac ccagcgtaat gataacgtcg tgcgccagcg caataacgac
    23941 ccctgccgcc agtcgccact caaagcgaaa acctacgtac acaaggatag acagcagcgc
    24001 cgccatcaac gccatcgcac cggtttgcgc aaggtctgcc cccacgctcg gaccgacgaa
    24061 ctcaatacgc ttcactgctg cattctgatt ggtggattcg ttaatcacct tcagaacctg
    24121 gctgcccagc acctgaccgc cggtttcgcc ttcagcaggc ggcatacgga ccatgatgtc
    24181 gtggctgcta ccaaagtttt gcagcattgg ctcttcaaaa ccggcttttt gcaatgcatc
    24241 acgcattacg tcaatttcag ccggtttttc gagcgtaatt tcgataaccg taccaccggt
    24301 gaaatccagc ccccagttaa agccgcgcac gcccataata acgatagcgg cgattaacag
    24361 cagaccagag atgccgaaag cccagtagtc ccagcgcata aagtcataga ctttacggcc
    24421 gtggtttagt tgttcaacag tatattcctg tgccacatcg cactcctcag attgacagct
    24481 tcttgacgcg cttgccgcca tatagcaggt ttacgatggc acgcgtaccg acaatcgcgg
    24541 taaacatcga cgtcgccaca ccgataccgg tagtaatcgc gaacccttta attgccccgg
    24601 tacccactgc gtacaggatg atgactttaa tcagcgtggt gatgttcgca tcgaagatag
    24661 aactgaatgc gccacgataa ccttcatcaa ttgcctgttg aacagtacgt ccgttgctca
    24721 actcttcttt aatacgttcg ttgatcagta cgttcgcatc gaccgccacc gcaagggtta
    24781 agacgatacc cgcgatacct ggcatactca gcgttgcgcc tggcaacagc gacataatgc
    24841 cgacgattaa gatcaagttg gcaatcagag cactggtcgc aatcagacca aacttcttat
    24901 agaagatgat catgaacaga atagaaacca gcagaccggc aaggcaagcc tccagcccct
    24961 gttcaatgtt ctgcataccc agggttgggc caatggtacg ttcttcaaca atctgaatcg
    25021 gcgcgatcaa cgcaccggca cgcagcagca gtgacagctg acgggcttcg ttcgggttgt
    25081 tgatgccggt gatacggaag ctgttaccca gacgagactg gatgttggcg atgttaatca
    25141 cctcttcctg tttcaccaga accgcacgac catttgcatc tttcttaccg ctgtctttgt
    25201 actccacaaa cagggttgcc atcggtttgc cgatgttgtc cttagtgaag ttagacatga
    25261 tgttaccacc agcgctatcg agcgagatgt taacctgcgg ttggttgtat tcgtcctggc
    25321 tggaagtgga gtcggtgata tggtcaccgg tcagaattac gcgtttgtac agcacaactg
    25381 gctgaccttc gcgggtctgt ttcacttcag agtcgcccgg tacgcgaccg gatgccgcag
    25441 cggcctggtc aacgttggtg tttaccagac ggaattccag cgttgccgtc gcacccagaa
    25501 tctctttcgc acgcgcagtg tcctgaatac ctggcagttc aacaacgata cggtcagcac
    25561 cctgacgctg aaccaccggc tccgccacgc caagttggtt tacacggtta cgcaggatat
    25621 tgatgttctg ctgcaccgca tattcacgcg cttcactcag acgagcatcg ctcattaccg
    25681 cacgcagctg gttgctgccc tggctactaa tcaccaggtc cggatggcgc ttgctcagat
    25741 acgcaatggc ttcatcacga gctttagcat cgcggaaagt aatgctcagg ccgtagttgt
    25801 tttctttacg aacggtggta tacgggatgc ccttttcacg cagatcgctg cgcaggctat
    25861 cgatattttg ttcctggagt ttgccaagcg cggtgtccat atccacttcc atcaggaagt
    25921 gaacgccgcc acgcaggtca aggccgagct tcatcggctc agcgtgaata gctgccagcc
    25981 agcgcggcgt tgccggggca aggttaagcg ccacgacgta tttgtcaccc ataacgccca
    26041 ttaatgcttc acgagcgcgc agctgggtgt cagtggagtc gaagcgcgca agaatagcgc
    26101 cctcttccag tgccacagac ttagcagtta ttttttcttc ttgtaacgtt ttctggacct
    26161 ggatcagcgt ttgctcactg gcggcgactc cgcgcgcacc agtgatctga acagccggat
    26221 cctcaccaaa caggttggga agcgcataca gcagaccgat gacaatcacc acgatcagca
    26281 tgacgtactt ccacaaagga taacggttta acacggcaat tcccttaggg aaaaatttta
    26341 attacagcgc cttcatggtg cctttcggca ggacggcagc tacgaagtca cgtttaataa
    26401 ctacttcagt ggtgtcattc agcgcgatag caatgtagcc gttttccgct actttggtta
    26461 cgcgaccaac caggccaccg ttcgtcagaa cttcatcacc tttggcaatg gagtccatca
    26521 gctttttgtg ttctttggtg cgcttctgct gtggacgcag gatcatgaaa tagaaaatca
    26581 gaccgaatac caccagcatc aaaatcaaag acatcgggct accttgcgcc ggtgcacccg
    26641 ttgccgctac cgcatcagaa ataaaaaagc tcattaaatt tccctcatta ttaatattaa
    26701 tcaacgttca aaggtggtac ttctcgcccc tgacgctggt aaaaatcagt tacgaagctc
    26761 tctaatttac cctcttcaat agccttgcgt aaacccgcca tcaaacgctg gtagtaacga
    26821 aggttatgaa tggtgttgag tcgcgcgcct aatatttcgt tgcaacggtc aagatgatgc
    26881 aagtaagcgc gtgaataatt gcgacaggtg tagcaatcac actcaggatc gagtgggcca
    26941 gtatcgctct tatacttcgc attgcggatt ttcaccacgc catcggtcac gaacaaatga
    27001 ccatttcggg cgttgcgggt tggcattacg cagtcaaaca tatcgatacc acgacgtacg
    27061 ccttcaacca ggtcttctgg tttaccaacg cccatcaggt aacgcggttt gtctgccgga
    27121 atttgcgggc atacatgctc cagaatgcgg tgcatatctg ctttcggctc acccacagcc
    27181 agaccgccga cagcgtagcc atcaaaaccg atatctacca gacctttaac agaaatatca
    27241 cgtaaatctt cgtaaacgct gccctgaatg ataccaaaca gcgcattttt gtttccgaga
    27301 ctgtcaaaac gctcacggct acgcttcgcc caacgcagag acatctccat ggagcgtttt
    27361 gcgtaatccc agtcagcagg atacggcgta cactcatcaa agatcatgac gatatccgaa
    27421 ccaagatcgt actgaatctc cattgatttt tcaggatcga ggaaaatcgg atcgccgttg
    27481 atcgggttac ggaagtgcac gccctgttcg gtgattttac gaatatcgcc aaggctgaag
    27541 acctggaagc cgccggagtc ggtgaggatc ggccccttcc actgcataaa atcgtgcaga
    27601 tcgccgtgca gtttcatgat ttcctggccc gggcgcagcc acaggtggaa ggtgttgccg
    27661 aggataattt gcgcgccagt ggcttcaact tcttccggcg tcatcccttt tacggtgccg
    27721 taggtgccaa caggcataaa acaaggcgtt tccactacgc cacgatcaaa gaccaggcgg
    27781 ccacggcgtg cgcgaccgtc ggtggtgtcc agttcaaatt tcattttttc tccactacgt
    27841 cagaaaaaca gtccaacgtt ttaaaccagc gccgcggaat tactccccga cgcgctcatt
    27901 aattgcctgc ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt
    27961 ctcttctacc gctgctttat aggcattcat ggtgtgttga taaccggcaa aggccgaaac
    28021 cagcataatc agcgtcgatt ccggcaggtg gaagtttgtc accagcgcat cgaccacttt
    28081 gtactggaag cccggataga taaagatttg ggtatcgtca aagaacggtt cgataagatc
    28141 gtttttcgcc gcctgagccg cgctttccag cgaacgtact gaggtggtgc caaccgcaat
    28201 cacccggtta ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggcacttc
    28261 agcgtattcc gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg gctggaaggt
    28321 gcccgcacca acgtgcaacg tcacaaacgc catctccacg cctttggcgc gtaatttttc
    28381 cagcaaaggt tcgtcaaaat gcagacctgc ggtcggggct gcaaccgcgc ccggtttttc
    28441 gctataaacg gtttgataaa gttcgcggtc agcgtcttcg tccggacggt cgatatacgg
    28501 cggcagcggc atatggccga tgctgttgag aatatccagc accgagcgtt catcattaaa
    28561 ttcgacttca aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac tttcgtcatc
    28621 gcccagcagc agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat
    28681 gcgtttgtcg tcgagcatcc gttcaaccag cacttcaatc ttgccgccgc tggctttacg
    28741 cccaaacagg cgcgccggga tcacgcgggt attattaaaa accagaagat cgccggggtt
    28801 gagcttatca agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg gcccgtccag
    28861 cgacagtaaa cgacagctac tgcgttcagg catgggatag tgggcaatca gggattcggg
    28921 caattcaaag gagaaatcgg taacgcgcat gacactgact cagactaaaa taagaggcgg
    28981 gtagtctagt gccggggcgc tctccctgca acctttaccc ccttttggat agaatataat
    29041 cgatgaattt tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttccggca
    29101 atttactggc tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg
    29161 ctggcattca tatgcatcga cgtatcgacg tattaaccga caatctgccg gaagtccgcg
    29221 aagcacggga atggtttcgt agtgaaactc gccgcgttgc gcctattacg ctggatgtca
    29281 tgtgggatca ctttctttcc cgccactggt cgcagctgtc gccggacttt ccgctacagg
    29341 aatttgtctg ttatgcccgc gagcaagtga tgacgatttt gccggactca ccgccacgtt
    29401 ttatcaatct gaacaattac ttgtggtcag agcagtggct ggtgcgctat cgcgatatgg
    29461 atttcatcca gaacgtgtta aacggcatgg caagccgccg cccacgtctg gatgccctgc
    29521 gtgactcctg gtacgattta gacgctcatt atgacgccct cgaaacccgc ttctggcagt
    29581 tttatccgcg gatgatggcg caggcgtcac acaaggcgtt ataaaacgtt agttcatcca
    29641 taccgtagcc gaaatggcag gcaaagccag aatcccgtca gtcagttgcc catgcccttc
    29701 tttgcattgc cattgcacgg cattgagcaa cggtgacgcg ggtagcacga cttcacaagc
    29761 ttcgccacgg ttgattgcca ccagtacacg ctgctgattc agcacgcgga caaataccac
    29821 cacgttatct tccgcataca gcacctgaca gccgccatga cgtagcgcct gacttttctt
    29881 acgcagcgca atcattcgct ggtacagcgc gaataacgcc gtatcctgct tttccacctg
    29941 ccaagggaac ggtttacggc aaaacggatc gtttttgcca tccagtccta cttcatcacc
    30001 gtaataaatg cacggtacac caggccaggt gaacagccag accaccgcca gcggcaggcg
    30061 cgcaatatcc cgaccgagca gcgttttaaa tcgcgcagta tcgtggctgt cgagctgatt
    30121 aaacatacgt aattgttgtt gatgagaaag ccctgcgcgg taattatcca tccaggccat
    30181 acaggtttgg gcatcaattt gctgcggatc gtaagagata tcggtattgg caagaaatcc
    30241 ccacaacggg aatgtgaagc cacgatagtt catggcggca tcttccacat cggcctgtaa
    30301 ccattgccgt gcatcgccaa aatgttcgcc gacaatatac gcttccggct gggtttcttt
    30361 cgccgcttcg gtgatcccag caacgtgctg catattattg cgcgccccac ccgcctcccc
    30421 cagcatatgc accacatcca gccgccagcc gtccatactc cacggcgctt tcagccaatg
    30481 gcggacaata ctgtcttccc cgcgataaat ttcattcacc agactttccg actgataatc
    30541 cagcttcggc aagctggcat agccaagcca gtcgagcgcc gtgccatcat cactaaacga
    30601 gtaccagtcg cgccagggcg attcagggtt gtgacaagct ccccccgtgc cacgattgtg
    30661 cctgtcaaac caggcatggg aatcgccact gtggttaaac acgccgtcca gcaccagccg
    30721 cattcccagc tgctgcgtat tgtgtcgcaa acgcagcaac gccccatcac cgccaaactg
    30781 cggatcgaca tggcgataat cctcggtatc gtatttatgt acgctgggag ctttaaacac
    30841 cggattgaga tacagcgccg tcacgccaag ctttttcaga tacggcagtt tttcgcttat
    30901 cccgtccaga tcgccgccat agaacgttga tccgcccgcc tgcgccgtga ccggttcatc
    30961 ccagtcacgc aagatgatct cttgtccggc tgcatgatgg taatagacat gatcctgttc
    31021 agcttcacga ggaagactac gcgcaaaacg atcagggaag atctgataaa aaatctgatc
    31081 cgcagcccat tgtgggccga tatccggtac atcgacggca aactgctcca gtcgtgccgg
    31141 cggcattcgg ctgaagccct gcggtgtaaa ccaacgctgg cgatcgtgcc acagcagttt
    31201 gaaactgtaa cgccgccggg gttgtccgct ggagagatca atcgccgcac gccatgcggt
    31261 gacgccaggc tgctgctgac tgcgctgctt atgcatcgat actgacattt cttcgttatc
    31321 gtgttctgta cgcagcataa tgcgctgcgg tgggtcttcg cccgtcagcc acagtgtaat
    31381 gagcagttga tctttgcttt gtttaacaaa tgggggcacc ggcaggtgcc atgcatttaa
    31441 catcataatt cccctgtgat gaaaatctga tcaccttgcc atagcctaat gcaggatcac
    31501 tcatcatctg aaggatattt gggggaggag gacgggggcg gataactttc ggaatagccg
    31561 gatcaggatt tcctcctgaa ccggcttaca gaacgcaagt attattggtt ttcagccagc
    31621 tgacgatcgt ggcggcgttt aaacatccag ccaatcaaca gcacaacaat ccacgcgaaa
    31681 ccgacataca gcgagatacg cgtatccggg tgataaccaa tcaacccgat aataaagagc
    31741 aggaaaatca gcccgccgat ggtcgttgct accccacccg gcactttaaa tttcagcgcc
    31801 ttaacttctt ctggcggcaa acggcgacgg aaggcaattt gcgacagcag gatcataatc
    31861 cacacccaca ccgtggcgaa ggttgccagc gaagcgatca ccaggaagac gttttccggc
    31921 atgatgtagt tcagatacac cgcaaacagc agcgcggtag tcatcaccag caccgtaacc
    31981 cacggaatac cgcgacgcga cgttttgctg aaaattttcg gcgcgctgcc ctgctctgcc
    32041 ataccgtgga gcatacggcc tacgccaaat acatcactgt taattgccga cagcgaagca
    32101 gtcagcacaa caaagttaag aatgctggcg gcaaaggtaa tgcccatatg ctggaacgtc
    32161 agcacgaacg ggctaccggc agtgccaacc tgattccacg ggtagataga cataatgacg
    32221 aacagcgtac cgacgtagaa caccagaata cgcatcggca cggagttaat cgcacgcggt
    32281 atcgatttct caggatcttt cgcttcaccg gcggtaatcc cgataatttc gatcccaccg
    32341 taagcaaaca tcaccatttg caacgacatt accatgccaa gccagccgtt actgaagaag
    32401 ccgccgttgc tccacaggtt atgaataccg gtcggttgcc cgccgttgcc aatcccccag
    32461 atgatgatgc cgaaaccggc gacaatcatg atgatgatgg tggcgacttt aaagaacgag
    32521 aaccagaatt ccagctcacc gaagaccttc acgctcatca ggtttacggc gcaaatgatc
    32581 agcaccacgc tcagtaccca aatccagtgc ggcaccgtcg ggaaccagac acccatatag
    32641 ataccaaaag cggtcacatc ggcgatggcg acaataagga tttcaaagca gtaggtccat
    32701 ccggtaatgt aacctgccag cgggccgagg ttttcctgcg cataacgcga gaaagagctg
    32761 gcggccgggt tatgtaccga catttccccc agcgcacgca taatgatata cgccgcgata
    32821 ccaccgataa tataggccaa caacacgctc ggaccggcca ttttgatggc gtctgccgaa
    32881 ccgtaaaaca gcccggtgcc aattgctgaa cccagtgcca taaagcgtat gtggcgggta
    32941 cttagcccac gctttagctt gttcttactt tccatcaatt cctaacccat caacacaata
    33001 aaaaaccacg gggcatcgca gccccgtggt taaaacaaat gttcagtgat ttagtgagcg
    33061 ctggaggtca cctgacgacc tgccgcgcga tcccagataa tggccagaac caccatcacc
    33121 actgttggca ttaaccacgc cagaccttgt tcggccagcg gtaaacgctg cgcccaggac
    33181 ggtaagatat cgctgaatgc agatgctttg atcccgtcga gaataccaaa aagcaggctg
    33241 ataaacatcg gcggagcaat cacgcgggac gaattatgcc accatgagcg tgtaaaactt
    33301 aatacaacta gtgcgataca cggcggataa atagcggtca gcaccggtac ggagatctgg
    33361 atcagctggc ttaagccgag gttagaaacc accatcgaga agccgccgag gataaacacc
    33421 agcgtacgat aagagagcgg tacgtactgg gcaaagaatt ctgcacaagc acaggtcagg
    33481 ccaactgccg ttaccaggca ggcgatgaag attaacgccg ccagcaggaa gctaccgccg
    33541 ccgccaaagg tgtgctgaac gtaagcatgc agaatagcag cgccgtttgc agactgatcg
    33601 accagcgacg cgctgtctga acccagacgg aacagcgcca ggtacagcag agtcagacca
    33661 acacccgcca tcaggccagc ccagacggta taacgggtca gcagacgcgc ttcggtaacg
    33721 ccacgagaac gcgccgcgtt aacaataacg ataccaaaca ccattgcgcc cagcgtatcc
    33781 atggtcagat agccgttaac gaagccgtta gaaaacgcag cgttttgata agcctcagtc
    33841 gccgtgctga tagaacccgc cggccaaaca atcgcggcaa cagacaggat gaccagcgcg
    33901 ataattttca gcggcgcaag gaagttgccc acggtatcca gcagcttgcc cggatagagc
    33961 gaaaccagaa taacgatagc gaaatagacc aggctgtaga taaacagcgg cagcgcggaa
    34021 tcaccagtca gcggcgcaat ccccacttca aaggaaacgg tagctgtacg cggcgtagcg
    34081 aaaagcggcc ccaccgccag gtaacaaacc gttgccagca gcacgccagc gactttaccg
    34141 attggcgtgc tgaggctgtc aacaccgccg ccaacttttg ccagcgccac taccgttaat
    34201 accggcaggc caacggcagt aatgaggaag ccgaatgccg cagtccagac gtgttcgcct
    34261 gcctgcaagc cgaccattgg agggaaaata atgttacctg cgccgacgaa caacgcaaat
    34321 gtcataaagc ccagagcgat gatatcgcgc gatcttaatt gatgggtcat aaatcatact
    34381 gcctgtggat gtggtgttgt aacgttaaat ttttgtgcca atccctgggg atgatacagc
    34441 ggtatattta aacaatttta gcgggaaata ctcccaacca ggcgcggcga agaatagttt
    34501 ttcgatttac cgcgttaatg cagtcagtat gacagcacct gaagcgcaat ttaaacgctt
    34561 ataacgtttt aaagcaagga gggatctaaa aaccagaggt atatagcgat aagaaatcac
    34621 caaccagtat aaaacactat ttccgtgaaa aagacatggc taatcatgcg aacaaaaaat
    34681 caccaaccgt tgtaaaatct aacgcccata ttttgactgc aaagaaaata agccagcatc
    34741 ggctggctta tggcaataaa agatgacaaa ggcggattaa tcgctgtttt tggcaattaa
    34801 acgttccggg ataacaaaac tgaaacgtgt tccttttcct actgtactct caatattcag
    34861 gcgactttcg tgatgattca cagcatgttt cacgatcgct aaccctaatc cgctaccgcc
    34921 ggtttgccgg gaacgcgctt tatcaacgcg ataaaaacgc tcggtcaggc gcggaatatg
    34981 ctccgctgca atgcccggtc cgttatcttc aacgctaaat tcggcaccgt gcggcactcg
    35041 ctgccagcgt acggtgatat gcgtgccttc cggcgtatga ttcacggcgt tatagaccag
    35101 gttcgaaatc gcactgcgta gctgatcttc gttgccagac accttgaggc cgctatctat
    35161 ctcaaaggta aatgtctgtt ttttctgact cagagtctga gcctcacgct caacaacgcg
    35221 cagcatcatc ggcacatcaa ccttttcatt gagcaaatgc gtcggtgcgg cttctatttt
    35281 cgacagcgtc agcaactgct tcaccagccc ttccatccgc tgggtctgct cgcgcatggt
    35341 gtgcaacgct ttttcgcgta ccgcgccttc cagcggctgc tcatccatca tctccaggta
    35401 accctgtaac acggtcaatg gcgtacgtaa ctcatggctc acgttggcaa aaaagttacg
    35461 ccgcgcccct tccagttgat gcatttgcgt gacatcacgc gccaccatca gcaactgttt
    35521 gtgggtataa ggcatgacgc gaatttccag atgccgcccg gtgttgagca ccagattgag
    35581 cgggcgagaa aaatcacgcg ttttcaaata ttgcgtaaac tccgggtaac gcagcaggtt
    35641 aaggatgttc tgcccgttat cttccggcca gcgcaaacca agaatctgtt gcgccagacc
    35701 gttacaccag aaaataccgc cctcttccgt ggtcagcacc accgcgtcgg gcagcgactc
    35761 cgcgccgcta cgaaagcgtt taatcagatt gcccagttca cggcggcgtt ttttatttcg
    35821 cagctgcatc tggtgtaagc cgtatagtag cggttcccag ctaccacgcc ccggtggcgg
    35881 ggtcatactg cgatccaccc acagccacca tgaaaggcgc aataaattcc agaaatgcca
    35941 gataagcagt cctgttaccg atgccagcaa aaaccagggc aggtaaccaa aaaatgcacc
    36001 caggatgaaa gccgggaggc agcaaagtag cagctccagc accagccttt tccacgacag
    36061 ccgttccagc acgtaagata ctccagttaa gaaatcataa gccctgctct gcgtccgatg
    36121 agcaaggcgt taaaagcggg ttgaaaaacg atatcctgta ccgcgcacgg tctgcaccat
    36181 gcgatcatgc ccgcctggct ccagtgcttt acgcaggcga cgaatgtgga catcgaccgt
    36241 acgatcttcc acatacacgt tggttcccca gacgtggttt aacagctgct cgcggctgta
    36301 tacgcgctca ggatgcgtca taaaaaagtg cagcagttta aattctgtcg gccccatctc
    36361 cagcggctct tcacccgcca tcacccggtg agatgtcggg tcgagactta gcccctgcat
    36421 ctcgatcacc tcttccaccg ccattggcga aatacggcgc attaccgctt tgattcgcgc
    36481 caccagctcc ttcggcgaaa acggcttggt gatatagtca tccgccccgg tttcaaggcc
    36541 gcgcacgcga tcttcttctt cccctctggc ggtcaacatc accactggaa tatcccgggt
    36601 catcgactcg cgcttgaggt gtttgatgaa ctggataccg gagccgccag gtaacatcca
    36661 gtcaaggaga attaaatccg gccagggttc attcagttga ttcacagcac tgtcataatc
    36721 ttccgcttcg accggctgaa agccattttg ttcgagcacg aagcagacca tttcgcgaat
    36781 tggagcttca tcttctacga ccagaatacg tctcgccatg atttgccctg ttgtaataaa
    36841 taggttgcga tcattaatgc gacgtcatta tgcgtcagat ttatgacaga tttatgaaaa
    36901 gctcgtcgca catatcttca ggttattgat ttccgtggcg cagaaaaaag caaatggcac
    36961 atctgtttgg gtataatcgc gcccatgctt tttcgccagg gaaccgttat gcgcatcctt
    37021 cacacctcag actggcatct cggccagaac ttctacagta aaagccgcga agctgaacat
    37081 caggcttttc ttgactggct gctggagaca gcacaaaccc atcaggtgga tgcgattatt
    37141 gttgccggtg atgttttcga taccggctcg ccgcccagtt acgcccgcac gttatacaac
    37201 cgttttgttg tcaatttaca gcaaactggc tgtcatctgg tggtactggc aggaaaccat
    37261 gactcggtcg ccacgctgaa tgaatcgcgc gatatcatgg cgttcctcaa tactaccgtg
    37321 gtcgccagcg ccggacatgc gccgcaaatc ttgcctcgtc gcgacgggac gccaggcgca
    37381 gtgctgtgcc ccattccgtt tttacgtccg cgtgacatta ttaccagcca ggcggggctt
    37441 aacggtattg aaaaacagca gcatttactg gcagcgatta ccgattatta ccaacaacac
    37501 tatgccgatg cctgcaaact gcgcggcgat cagcctctgc ccatcatcgc cacgggacat
    37561 ttaacgaccg tgggggccag taaaagtgac gccgtgcgtg acatttatat tggcacgctg
    37621 gacgcgtttc cggcacaaaa ctttccacca gccgactaca tcgcgctcgg gcatattcac
    37681 cgcgcacaga ttattggcgg catggaacat gttcgctatt gcggctcccc cattccactg
    37741 agttttgatg aatgcggtaa gagtaaatat gtccatctgg tgacattttc aaacggcaaa
    37801 ttagagagcg tggaaaacct gaacgtaccg gtaacgcaac ccatggcagt gctgaaaggc
    37861 gatctggcgt cgattaccgc acagctggaa cagtggcgcg atgtatcgca ggagccacct
    37921 gtctggctgg atatcgaaat cactactgat gagtatctgc atgatattca gcgcaaaatc
    37981 caggcattaa ccgaatcatt gcctgtcgaa gtattgctgg tacgtcggag tcgtgaacag
    38041 cgcgagcgtg tgttagccag ccaacagcgt gaaaccctca gcgaactcag cgtcgaagag
    38101 gtgttcaatc gccgtctggc actggaagaa ctggatgaat cgcagcagca acgtctgcag
    38161 catcttttca ccacgacgtt gcataccctc gccggagaac acgaagcatg aaaattctca
    38221 gcctgcgcct gaaaaacctg aactcattaa aaggcgaatg gaagattgat ttcacccgcg
    38281 agccgttcgc cagcaacggg ctgtttgcta ttaccggccc aacaggtgcg gggaaaacca
    38341 ccctgctgga cgccatttgt ctggcgctgt atcacgaaac tccgcgtctc tctaacgttt
    38401 cacaatcgca aaatgatctc atgacccgcg ataccgccga atgtctggcg gaggtggagt
    38461 ttgaagtgaa aggtgaagcg taccgtgcat tctggagcca gaatcgggcg cgtaaccaac
    38521 ccgacggtaa tttgcaggtg ccacgcgtag agctggcgcg ctgcgccgac ggcaaaattc
    38581 tcgccgacaa agtgaaagat aagctggaac tgacagcgac gttaaccggg ctggattacg
    38641 ggcgcttcac ccgttcgatg ctgctttcgc aggggcaatt tgctgccttc ctgaatgcca
    38701 aacccaaaga acgcgcggaa ttgctcgagg agttaaccgg cactgaaatc tacgggcaaa
    38761 tctcggcgat ggtttttgag cagcacaaat cggcccgcac agagctggag aagctgcaag
    38821 cgcaggccag cggcgtcgcg ttgctcacgc cggaacaagt gcaatcgctg acagcgagtt
    38881 tgcaggtact tactgacgaa gaaaaacagt tacttaccgc gcagcagcaa gaacaacaat
    38941 cactaaactg gttaacgcgt ctggacgaat tgcagcaaga agccagccgc cgtcagcagg
    39001 ccttgcaaca ggcgttagcc gaagaagaaa aagcgcaacc tcaactggcg gcgctcagtc
    39061 tggcacaacc ggcacgaaat cttcgtccgc actgggaacg catcgcagaa cacagcgcgg
    39121 cgctggcgca tactcgccag cagattgaag aagtaaatac tcgcttacag agcacaatgg
    39181 cgcttcgcgc gagcattcgc caccacgcgg cgaatcagtc agcagaatta cagcagcagc
    39241 aacaaagcct gaatacctgg ttacaggaac acgaccgctt ccgtcagtgg aacaacgaac
    39301 tggcgggttg gcgtgcgcag ttctcccaac aaaccagcga tcgcgagcat ctgcggcaat
    39361 ggcagcaaca gttaacccat gctgagcaaa aacttaatac gcttgcggcg atcacgttga
    39421 cgttaaccgc cgatgaagtt gctagcgccc tggcgcaaca tgccgagcaa cgcccactgc
    39481 gtcagcgcct ggtcgcgctg catgggcaga ttgttcccca acaaaaacgt ctggcgcagt
    39541 tacaggtcgc tatccagaat gtcacgcaag aacagacgca acgtaacgcc gcacttaacg
    39601 aaatgcgcca gcgttataaa gaaaagacgc agcaacttgc cgatgtgaaa accatttgcg
    39661 agcaggaagc gcgcatcaaa acgctggaag ctcaacgtgc acagttacag gcgggtcagc
    39721 cttgtccact ttgtggttcc accagccacc cggcggtcga ggcgtatcag gcgctggagc
    39781 ctggcgttaa tcagtctcga ttactggcgc tggaaaacga agttaaaaag ctcggcgaag
    39841 aaggtgcggc gctgcgcggg caactggatg cattaacgaa gcagcttcag cgcgatgaaa
    39901 acgaagcgca aagcctccga caagatgagc aagcacttac tcaacaatgg caagccgtca
    39961 cggccagcct caatatcacc ttgcagccac aggacgatat tcaaccgtgg cttgatgcac
    40021 aagatgagca cgaacgccag ctgcggttac tcagccaacg acatgaatta caagggcaga
    40081 ttgccgcgca taatcagcaa attatccagt atcaacagca aattgaacaa cgccagcagc
    40141 agctcttaac ggcattggcg ggttatgcac tgacattgcc acaggaagat gaagaagaga
    40201 gctggttggc gacacgtcag caagaagcgc agagctggca gcaacgccag aacgaattaa
    40261 ccgcgctgca aaaccgtatt cagcagctga cgccgattct ggaaacgttg ccgcaaagtg
    40321 atgatctccc gcacagcgaa gaaactgtgg cgctggataa ctggcggcag gttcatgaac
    40381 aatgtctcgc attacacagc cagcagcaga cgttacagca acaggatgtt ctggcggcgc
    40441 aaagtctgca aaaagcccag gcgcagtttg acaccgcgct acaggccagc gtctttgacg
    40501 atcagcaggc gttccttgcg gcgctaatgg atgaacaaac actaacgcag ctggaacagc
    40561 tcaagcagaa tctggaaaac cagcgccgtc aggcgcaaac gctggtcact cagacagcag
    40621 aaacgctggc acagcatcaa caacaccgac ctgacgggtt ggctctcact gtgacggtgg
    40681 agcagattca gcaagagtta gcgcaaactc accaaaagtt gcgtgaaaac accacgagtc
    40741 aaggcgagat tcgccagcag ctgaagcagg atgcagataa ccgtcagcaa caacaaacct
    40801 taatgcagca aattgctcaa atgacgcagc aggttgagga ctggggatat ctgaattcgc
    40861 taataggttc caaagagggc gataaattcc gcaagtttgc ccaggggctg acgctggata
    40921 atttagtcca tctcgctaat cagcaactta cccggctgca cgggcgctat ctgttacagc
    40981 gcaaagccag cgaggcgctg gaagtcgagg ttgttgatac ctggcaggca gatgcggtac
    41041 gcgatacccg taccctttcc ggcggcgaaa gtttcctcgt tagtctggcg ctggcgctgg
    41101 cgctttcgga tctggtcagc cataaaacac gtattgactc gctgttcctt gatgaaggtt
    41161 ttggcacgct ggatagcgaa acgctggata ccgcccttga tgcgctggat gccctgaacg
    41221 ccagtggcaa aaccatcggt gtgattagcc acgtagaagc gatgaaagag cgtattccgg
    41281 tgcagatcaa agtgaaaaag atcaacggcc taggctacag caaactggaa agtacgtttg
    41341 cagtgaaata actattcagc aggataatga atacagaggg gcgaattatc tcttggcctt
    41401 gctggtcgtt atcctgcaag ctatcacttt attggctacg gtgattggta gccgttctgg
    41461 tagttgtgat ggtggtatga aaaaagtcat tttatctttg gctctgggca cgtttggttt
    41521 ggggatggcc gaatttggca ttatgggcgt gctcacggag ctggcgcata acgtaggaat
    41581 ttcgattcct gctgccgggc atatgatctc gtattatgcg ctgggagtgg tggtcggtgc
    41641 gccaatcatc gcactctttt ccagccgcta ctcactcaaa catatcttgt tgtttctggt
    41701 ggcgttgtgc gtcattggca acgccatgtt cacgctctct tcgtcttacc tgatgcttgc
    41761 cattggtcgc ctggtttccg gctttccgca tggcgcattt tttggcgtcg gcgcgatcgt
    41821 gttatcaaaa attatcaaac ccggaaaagt caccgccgcc gtggcgggga tggtttccgg
    41881 gatgacagtc gccaatttgc tgggcattcc gctgggaacg tatttaagtc aggaatttag
    41941 ctggcgttac acctttttat tgatcgctgt ttttaatatt gcggtgatgg catcggtcta
    42001 tttttgggtg ccggatattc gcgacgaggc gaaaggaaag ctgcgcgaac aatttcactt
    42061 tttacgcagc ccggccccgt ggttaatttt cgccgccacg atgtttggca acgcaggtgt
    42121 gtttgcctgg ttcagctacg taaagccata catgatgttt atttccggtt tttcggaaac
    42181 ggcgatgacc tttattatga tgttagttgg gctagggatg gtgttgggaa atatgctaag
    42241 tggcaggatt tcaggacgtt attcaccact gcgcattgca gcagtgactg actttataat
    42301 tgtactggca ctgctgatgc tctttttctg cggcggcatg aaaacaacgt cgcttatttt
    42361 tgcttttatt tgttgcgcgg gattatttgc cctttcagca cctctgcaaa tattattact
    42421 acaaaatgcc aaaggcggag agttattagg tgccgcaggt gggcaaatag cgtttaacct
    42481 tggtagcgcc gtcggcgcat attgcggtgg tatgatgctg acgctggggc tggcatataa
    42541 ttacgtggcg ctgcctgccg ccctgctttc gtttgctgcg atgtcgtcat tgctgctgta
    42601 tggtcgctat aagcgccagc aagcggcgga tagtccggtg ctggcgaaac cactggggta
    42661 gattgcagtc acgttgcgcg ttgcttattg ccggatgcgg cgtgaacgcc ttatccgccc
    42721 tacgcggttc cggcacattt tgtaggcctg ataagacgcg gcaagcgtcg catcaagcat
    42781 gatgcgccaa ttgcctacgt tttttactct tgtggccata accacgcagc gccgcgtacg
    42841 ccgctggaat caccgtgctt cgccttacgc accggcgttt cacattcgcc gccgaagaca
    42901 aattgtttaa tcaactgccc aaccgtttga tataaacggt ctacattgct catcccgccc
    42961 cccaggacaa tcacatccgg atcgagaata ttcacgacat gtgccagcga ttttgccagc
    43021 cgcagctcgt agcgacgcaa tgccagttcc gctaccggat cgctttcttc aaccaggcgg
    43081 ataatttcac tgcctttcag cgcatgtccg ctcaaacgac gataatcagt cgcgaatccc
    43141 gtgcccgaaa taaaggtttc aatacaacct tgtttaccgc aataacaagg gacttcctcg
    43201 cgataacgca gttcgtcttc gtccatccac ggtagcggat tgtgtcccca ctcacctgcc
    43261 gtgccattgc cgccgatatg cgcccgccca ttgaatgcca cgcccgcgcc gcatcccgtg
    43321 ccgataatca cggcaaatac tgtctgcgct cccgctgccg cgccatctac tgcttctgaa
    43381 accgccagac agttggcgtc atttgccagc cgcacttccc gctgcaacct cgcgcttaag
    43441 tctttatcga atggctgacc gttgagccag gttgaattgg cattcttcac cacaccggtg
    43501 taaggcgaaa ttgagccagg aatgcccata cctaccgttc cgcgctgccc tgtcgcctgc
    43561 tccgccatat caaccaacgt ggcgatcgtt tcaatagttt gccggtaatc atcacgcggc
    43621 gtgggcagac gatggcggta caactgctcc cctgcatcgc ccagtgcaat cacttcagtt
    43681 ttggtgccgc ctaaatcgat acctatacgc acggtactct ccttattttt ttcaatatca
    43741 atagcgtaga gacggacaac cggattggca atgcaaggcc gccgacaatt cgttatcatg
    43801 cccgctaaat ttaacgacaa ggccgtggaa attatcatgc tgtggttcaa aaatttaatg
    43861 gtttaccgtc ttagccgcga gatttcgctg cgtgcagaag agatggaaaa acagctagcc
    43921 tcgatggcat ttaccccatg cggcagccag gacatggcga agatgggctg ggttcctccg
    43981 atgggatcgc acagcgatgc gttaacgcac gttgccaatg gtcaaattgt tatctgcgcg
    44041 cgcaaagaag aaaaaatcct cccgtctccg gtgattaaac aggcgcttga agcgaaaatc
    44101 gccaaactgg aagcggaaca ggcgcgtaag ctgaagaaaa ccgaaaaaga ttcgctgaaa
    44161 gacgaagtgc tgcactctct gctgccgcgt gctttcagcc gttttagcca gacaatgatg
    44221 tggatcgaca cggttaacgg tttgattatg gtggactgcg ccagtgccaa aaaagcggaa
    44281 gatacgctgg cattactgcg taaaagcctg gggtcgttac cggttgtacc attgagcatg
    44341 gaaaacccga ttgaactgac gctgaccgaa tgggttcgct ccggtagtgc ggcacagggc
    44401 ttccagctgc ttgatgaagc cgagctgaaa tcgttgctgg aagatggcgg cgtgatccgc
    44461 gcgaagaaac aagatctgac cagcgaagag atcaccaatc acattgaagc cggaaaagtg
    44521 gtgactaaac tggcgctcga ctggcagcag cgcattcagt ttgtgatgtg cgacgatggt
    44581 tcgcttaagc gtctgaagtt ctgcgacgag ctgcgcgatc aaaacgaaga tatcgaccgt
    44641 gaagatttcg cccagcgttt tgatgccgat ttcatcctga tgactggtga actggcagcg
    44701 ttaattcaaa acctgattga aggattaggt ggcgaagcac aacgttaatt gctgattttc
    44761 ctttaatgcc ggatgcgacg cctgccgcgt cttatccggc gtacgaagcc acaccaggca
    44821 tataattatt cgctacggcg ggcaataatt tttagcgcag caatattatg cgttttatgc
    44881 tgtaactgat ctaatcgcct catccctatt cgcctgtcag cagcacgcag aatattcact
    44941 aacccccatt tatcgctggt aaattgtttt gttaacaact ccgcttttgg ggtgtcaata
    45001 tattcacgca ataaatcaga aattgaacaa atatccgtca ggtaaggata ccagcaagtt
    45061 tcaccgtgaa aattacgcac tccaccgtta cctgcaataa agtctttcgg gtaatcccag
    45121 atcacgtttt tatccaacgt gatccaataa cgcggtaaat cggtgcttcc accattttgg
    45181 ctgcgcatgg gataacgggt acagtgaatt tgcaggttga tcgttggcgt cagtaagtca
    45241 tagatctccc gctgtaactt gctccagggg gcgttgtgtc atcagttccg caaaaaatac
    45301 ctggagatct tcactgatat ttatatcaac cttgatgaat tatgaatata tcggaaattg
    45361 aaaaaatcga agcaaaggaa atattattat gggttaaaga gaggatattt cagaaaaatg
    45421 ccctcccgtt caaggggaag gcgaggaatt acagatagcg gcacagataa gaggtgggtt
    45481 cggcaacttg cagatgaaac tcactgtgac cgggaacatt aaacaccgaa ccggcttcat
    45541 acacttgcca gtcggtcgcg tcaggcagta acacattcag cgcgccactg attaccgtca
    45601 tctcttccgg ctcagcggtg ctgaaggtgt attcgccttc aaccataaca cccacgctgg
    45661 cgcgaccagt gctgctgctg gaaaagccga ttgatttcac tttgccggaa aagtactcat
    45721 tactttgaag cataaactgg ccctgtttgg tagttgaaca tctacttcac tataggggcc
    45781 agaggcgctg actgtcacgc aaaattacac cagtaattcc gcagccagcc gtgcaatcaa
    45841 tacgttagac agcaagacag gaacatcgag ctgtttttgc agtaaatcgc gatgacgttg
    45901 gtgaaatcct aaacaatcca gcatgatgac atcagcccct tttgccagta attctttccc
    45961 ggcatcaatg attttttgtt ctgaatcatg aatggggtta cccaatgaaa ataccggcga
    46021 tttctgcaaa atttgccatt tttgcgcctg aacgggcagc atctcctcaa ccggaacgat
    46081 aacccccacc tgatgatctt caacaataga ggaaaccagt ggaggcaata ttcgcgacgg
    46141 ctcaagaaag atcgtattac gcgcagtcat actactaatg tttgctgtac tcattaataa
    46201 aatgacgtca taaccctgat tatcgagcac ttcaaccaca ccttgcaggt ctcgctccac
    46261 tttacgacgc gaaacatggg ccagctggtt gtcatttaat aatgtgagaa tggtgtcttc
    46321 gcctgcttct ggcgcgtact ccgccatcac ttcttcacga cttaacttcc ccagcaggct
    46381 atgatgggaa atattatctt cgtcaatgta ttccgtcagg agcggcaaaa cctcctgcat
    46441 aggtacaatg ccgatggtca ggatcgccaa cgacgcactc attgttcatc caccttactt
    46501 ttctttcgtc gttaattacc gggcaagtgt gaagcaccac tgctgacact tacttgtgct
    46561 acaaatgaca aaaagcgtag cagcagacgt gcacggcata atgtaaagat tccaaatgat
    46621 tccagtaatg gattgttatt gtttaatatt ctaattatta gaaaaacatg aattatgaaa
    46681 aatgtgacgc agatcccttg tagtggcact cagaatccct tccgagcagt ctgatgcggt
    46741 cactctttgt cagggtcctc atagcgtttt ttcgcatcca tcgcttcctg agccgtcgga
    46801 tgttcactga tcaacgagtc tggtttagga tgatcggctc tgagttggta ccaggttacc
    46861 gtctgtcctg gctttccttt ttcaatcgtc actatatatg cttcacgagg ataaggcggt
    46921 ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt taagtatagg cgctctaaaa
    46981 tcaacaattg atcgtctgtg ccagggcgct acgaatttca gaaatcacct ggctgggttc
    47041 gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat agcgcatcgc gttcttccag
    47101 cacttcctga acttcttcgc tcagcggttt tcccgttaag gttggccgta aatcttcttc
    47161 cggtgcagct tgcagtcggt taaccaggac tgatactggc gcacacaaat aaaccacgat
    47221 cccgttattt tgcataaagt gacgattaaa ttccgtcaga ataatgccgc cgcctgtagc
    47281 gataacggtg gatggcgcag ttaccgcttc cagcgccgcc gtttctctgg cgcgaaatcc
    47341 cgcccactct tccctttcga cgatctccgc gaccgtcata ttgagctgtg attgcaacca
    47401 ctgatcggta tcgacaaacc gacggttaag cgaatcggca agggccattc cgaccgttgt
    47461 tttaccacag ccccgaggcc cgatcagaaa aagaggttgt gtcatcgtgg gttttcccca
    47521 ataggtcgca atgcggcgaa agccggtgtc atgagaatag cgatcatact atcaaactag
    47581 tacaatttcg attgtaaaga aaaaattcca cttaaagtga aaatctcaat acatccctta
    47641 ctataccaat aaatattcaa gaatgaagtg taaataataa attacattta gccacgacta
    47701 cgttgcactt ccagccacca cttctccagc tccgcggcaa aggcctggcg gtcacgttgt
    47761 gaaaggctat ctggtccgcc ggtctggatc ccactggcac gtaaggtatc cataaaatcg
    47821 cgcatcgtca ggcgctcacg aatggtcgct ggcgtgtaac gttcgccgcg cggattaagc
    47881 gccgctgcgc ctttctcgat ggcttcagca gccaaaggta tatctgcggt gatcaccaaa
    47941 tcgcccgctt cacactgccg gacaatttcg ttatcggcaa cgtcgaaacc tgccgcgacg
    48001 cgcagcgtac gaataaatcg cgatggcggc acgcgtaaac tctggtttgc taccagtacc
    48061 agcggcatct gcatacgttc cgccgcgcga tacaaaatct ctttaattac attgggacac
    48121 gcgtcggcat ccacccaaat tgtcataaag tcatcctttg ttgggtaatc ctctattgtg
    48181 tcgcgctttt gccttccggc atagttctgt ttatgcttct gccagcgatt atcaaaacaa
    48241 tgaatttcac ggcaggagtg aggcaatgga aaagaaaatc ggttttattg gctgcggcaa
    48301 tatgggaaaa gccattctcg gcggtctgat tgccagcggt caggtgcttc cagggcaaat
    48361 ctgggtatac accccctccc cggataaagt cgccgccctg catgaccagt tcggcatcaa
    48421 cgccgcagaa tcggcgcaag aagtggcgca aatcgccgac atcatttttg ccgccgttaa
    48481 acctggcatc atgattaaag tgcttagcga aatcacctcc agcctgaata aagactctct
    48541 ggtcgtttct attgctgcag gtgtcacgct cgaccagctt gcccgcgcgc tgggccatga
    48601 ccggaaaatt attcgcgcca tgccgaacac tcccgcgctg gttaatgccg ggatgacctc
    48661 cgtaacgcca aacgcgctgg taacccctga agataccgct gatgtgctga atattttccg
    48721 ctgctttggc gaagcggaag taattgctga gccgatgatc cacccggtgg tcggtgtgag
    48781 cggttcttcg ccagcctacg tatttatgtt tatcgaagcg atggccgacg ccgccgtgct
    48841 gggcgggatg ccacgcgccc aggcgtataa atttgccgct caggcggtaa tgggttccgc
    48901 aaaaatggtg ctggaaacgg gagaacatcc gggggcactg aaagatatgg tctgctcacc
    48961 gggaggcacc accattgaag cggtacgcgt actggaagag aaaggcttcc gtgccgcagt
    49021 gatcgaagcg atgacgaagt gtatggaaaa atcagaaaaa ctcagcaaat cctgatgact
    49081 ttcgccggac gtcaggccgc cacttcggtg cggttacgtc cggctttctt tgctttgtaa
    49141 agcgccaaat ctgccgattt caaccactca cgatagtgac tcatttgtgg gttcagcggc
    49201 gcaaccccca cactaatccg taaagttacc tgtggcgtat tcggcaagcg taacgtgttt
    49261 agcccttcac gcacccgtaa catggcggta atggcgctct cagctggcgt accggacatg
    49321 attactgcaa actcatcgcc gccaaaccga ccaatcacat cgctaccgcg cagggtaatt
    49381 tgtaactgtc gggtaagcgc cacaatcgct tcatcgccca catcatgacc ccaggtatcg
    49441 ttgatgctct tgaaatggtc gatatcgata atcagtaacg ttgcatcgcg attatgccgc
    49501 cgacagttat caaattcatt gcgtaacata gtttcccaat gacgtcggtt atacacgccc
    49561 gtcatgccgt cgcgggtact catgacctgc aacctgcgtt tatgttccgc cagtttggtt
    49621 gccgtctggt agctgaccca gccaaacagc agaggataaa cgacaataat gggaagggag
    49681 agccaccatt ccagcggcgc actattgaac gacacggtaa tgcccgtaag ctcgagggtg
    49741 acaaggcagg aaaccaccat caacaccaga cccgcgacaa acagacgggg gccgcctgcc
    49801 cccatcaaat tcagacacat aatcatcaac atagcggtgg aaggcagcac gtttacgccc
    49861 attacgccta cccacattcc cgctaatact gcatcggttt ttaagttgta aatttcccgg
    49921 ctaagcggat cgacggccct gctcgctatc tgccaggcta aatgcggcca gacgaacgcc
    49981 cagccgacca acaccagcca ccaccagccc ggcggcgggt gcgaaaccag cgttgaagca
    50041 atcggtaaga acatgccagc caggccaacc gcacggggta gtctgacgcg acgggcgaag
    50101 cgcagcccgg accgctgatg ttcgttttga ggagttaaag gaggttcctc cccatgcgcc
    50161 gccgcttttt tgaaaaagtt ttcatcattc attatttttg ggaacattca gaaacaattt
    50221 tcccaaatta tagagacggg gctaaaggca gaagtatgaa atttcgggtg agccactttc
    50281 gtagctcacc cgttagcact atcaggtatc aggcggcttt cttgaggcag gcactcataa
    50341 acttattacg atcatcacct ttcagagatt gttgcgttgc ttgattatta cattcgcgca
    50401 tcttttgctg ctgtggcgtc aaactttttt caccaggcgc agacttgctg ttcttcaggc
    50461 aatcactcat gtaggtctta cgagcatccc ctttcaacgc ctgcgccgtc gcctgctgat
    50521 tacaggatgt aatacgctgt tgttgtgggg ttaaggttct ctcggcagcg ccgacggtgg
    50581 ttaaaaaaac cagaccaaaa agcaaggtaa ccagtaatgt tattttcata gcaccatccc
    50641 tcttcatgtt ttaaccatga gcgtatgcgc ccgtgatctg ccattaagtc tgattgctaa
    50701 cagcaaaaaa accacccggc agcgaaaatt cactgccggg cgcggtttta tttcagcccc
    50761 agggcggctt tcatggtgta gaagagatcg gtctggtcgg tcagtccaac aacattggcg
    50821 gcatgcgggc catacgccgc aatacgcaac tgactgccgg tatgttcttg tgaatcctct
    50881 tcggagttcc cgtagctcat caccatcact gcgccatctt tggtatttag cgcctgggtg
    50941 aggcccggag ctttggtatc cggcgcgaca atctggctgg cgtgggcgtg atcagcggtg
    51001 actatgacca gcgtattgcc atccttttta gcgaattcca gcgcccgttg tacggcttca
    51061 tcgagatcga ccgtctcgcc aatttgccca caaggattcg cagcgtgatc ctgtttatcg
    51121 attgacgcac cttcaacttg caggaaaaat cctttctcat ttttactcaa caattcaatg
    51181 gctttgtcgg tcatctgcgc cagggttggt acactgtcat tacgttgcgg attaggcgta
    51241 caggtgactg cgggcttatc gatattgccg tggtacgttg ctttcggtcc tagccagcgc
    51301 actggcatat tgccgtcagc aaacagtcca agcagaggtt tttgctgatt cgcttccgtc
    51361 accgaattca gtgaggcagc atcgctgacc aactgataac cacgcgcctg tgcctgttca
    51421 cgcagcgttt ttccctgcca ttcaccggcg gttgccgttt cagcaaaggt ttttgcgcca
    51481 ccgccaagcg taacgtcggc acgagcgtta agcagctgtt cggtaatcga tccttttccg
    51541 cctttttcca gagcgttacc cggacatttt tcactggtcg cgctcggacc gtagcatttg
    51601 cgcgaagtca catgtgccac cagcgcagcg ggcgtggcat cctgcaactc tgcggtagaa
    51661 acgttaccgg tcgccagacc tgcggctttt gccatttcca gaatcgttgg gtgatctttt
    51721 tcgtgaatat cgacgcccag cgcgccgtta taggttttga caccggttga ccaggcggtt
    51781 gctgatgcag ccgaatcggt gacgtagtcc ggtttgccgg tttttttatt cagcgcatag
    51841 tgagtgtatt gcccggtaag cggtaaggca tcgatacctt taaaaaagcc gcccgcacct
    51901 tcggcataat tacgtgcggc tgtaatttcc gaatccccca tcccatcgcc aatcagcaaa
    51961 ataatatttt ttgcaggttt atcgctaaga gaatcacgca gagcggcagt ctgatcaccc
    52021 gttaaacggc gagcaccgcc gggtgcagta atatcgccct gagcagcccg gttttccaga
    52081 acaggcattt ctggtgtccg ggcttttgtc acaggggtaa acagtaacga taagagtgcc
    52141 agtgcaatag tgctttgttt cactttattt tctccatgta caaatacatt aaaaaataaa
    52201 aacaaagcga ctataagtct cggccgtgac aactttatga cagctgttga aaagattaac
    52261 tttttactga cgaggatgct tcaataactt ctttacgtaa tcgcgcagca gctccgtatc
    52321 gtcgtcagga atgctggcat cgggctttac ctcgtacagc gccccctcta cctgatcaat
    52381 caaccgctgt tggtcatttt gcgccatatt gcgaagcatt gcagtgacga taatctccaa
    52441 agcttctacc tgcgcacaca gttctttcga ctcttcttct ttttgggcaa gcttaaataa
    52501 caactcagca atgagatttt tcatgtctgt atttccttat ccaaagtatg gagaagttaa
    52561 cattatgttt cacaacttca tagtgtctgg aagtattatt tttatgacaa aggaaatact
    52621 ttgcgcaaat attttttgtt tgattaccag cgaaacgttt ctcttattta aataactacc
    52681 cacacaaaat ataggctttt aattatcaac ctttattata aataaaagtt atgaaatata
    52741 ttctcttcgt taacttataa ttaagagaaa aaaactcacc gcatggccat ttaagccaga
    52801 aataaattag cttcccgttc atcatttttg tgatcttgcg cacatctttg agataacgca
    52861 ctttcttcac cagtacgttg cacgctttgt cagcagaaga ttgcaacaat ctttcagaag
    52921 gccgcgaatc ccccgttttg tcgtggtaat gtatgcactt tgtgttagac gggataggtt
    52981 tttaagatgg aaaaactgcg ggtaggaatc gtttttggtg gtaaatcagc ggaacatgaa
    53041 gtgtctctgc aatcggcaaa aaacattgtc gatgccattg ataaaagtcg cttcgacgtt
    53101 gtgctgctgg gcattgataa acaagggcaa tggcacgtca gcgatgccag caattatctg
    53161 ctaaatgcag acgatcctgc ccatattgcg ttgcgccctt cggcgaccag ccttgcgcag
    53221 gtgccaggta aacatgagca tcagcttatc gacgcgcaaa acggtcagcc gttgccgacg
    53281 gtggatgtca ttttcccgat tgtccacggt acgctgggcg aagatggttc cttgcaggga
    53341 atgctgcggg tcgccaattt accgtttgta ggttctgatg ttctggcttc agcagcctgt
    53401 atggataaag atgtcaccaa acgtctgcta cgcgatgccg ggctgaacat tgcgccattt
    53461 attaccctga cgcgcgctaa tcgtcacaac atcagttttg ccgaagtgga gtctaaactg
    53521 gggttaccac tgtttgtaaa accggctaat cagggctctt ctgttggtgt cagcaaagta
    53581 accagtgaag aacagtacgc aattgccgtc gatctggcgt tcgagttcga tcataaagtg
    53641 atcgttgagc aagggatcaa aggtcgtgag atcgaatgcg cagttctggg caacgacaat
    53701 ccgcaagcca gcacctgtgg cgagatcgta ctcaccagcg atttctatgc ctacgacacc
    53761 aagtacattg acgaagatgg cgcgaaagtg gtagttccgg cagccattgc accagagatc
    53821 aacgataaga tccgggcgat tgccgttcag gcttatcaga cgttgggatg cgcaggcatg
    53881 gcgcgtgtag acgtgttttt aaccccagag aacgaagtgg tgatcaacga gatcaacaca
    53941 ctgcctggct tcaccaacat cagtatgtat ccgaagctgt ggcaagccag cggtctcggt
    54001 tacaccgatc tgatcacgcg tttgattgag ctggcgctgg agcgtcacgc tgcggataac
    54061 gcattgaaaa ccacaatgta atattttaac gcccgataat attcttattg ggcgttttca
    54121 gtttcttcat cgcgacgacg aataatcagc cccgccagcc agaaactaat cacccacgtc
    54181 accagaccga cggcataggt ctgccaacct tttgcttcaa atcccagtaa ccccaccacg
    54241 ccattaagaa tgaatataag gccaatggcg aacgcatagt agtgccagtc acggcggatt
    54301 tttacaggca gattcatggc agctccgtta acgaaaaaca acatccttgc atattctgta
    54361 acaaccgtct gtggtgtaga caacactttc gtaattgtta ataaatttaa cttaaggttg
    54421 ccattttgtg actcacaacg aagattcacg cttcgggaat attcccaaca tcaatttacc
    54481 atgattctga tattgacaat ctaaatgacc tgtcagactt gccataagcc gctggtatca
    54541 caccagaaga gaagcagatg acttccggag gtttttatgg ctgatttcac cctgtcaaaa
    54601 tcgctgttta gcggaaaata tcgcaatgcc tcttcaacgc ctggcaacat tgcctatgcg
    54661 ttgtttgtgc tgttttgctt ttgggctggg gcgcaattgc tgaacctgtt agtgcatgcg
    54721 cccggcgtct atgagcgttt aatgcaggtc caggaaacag gtcgcccacg ggtggaaatt
    54781 ggtttaggtg tcggcaccat tttcgggctg atcccgtttt tagtaggctg cctcattttt
    54841 gcagtggtgg cgctatggct gcactggcga catcgccgcc agtaagattt acgttgccag
    54901 atacggccta aaacgcctta tctggcatga cattcctgtt tgatcactgt ttcatacaac
    54961 gcgcccgccg ttcgtctacg cgcttcgcaa accaggccgt agtcaggttg cgggtgattt
    55021 tcgggctttc cagttgaatg ccaggcaaca tttcgcgggg tagtgattta cccgttttcg
    55081 cttcggcaag ttgataaact ttcttgtaca gcgccgtctc ctcaaaagag aagctgtcac
    55141 ctttctctaa ctgacggcga atttcgctgt cgctcatccc cagttttgcc gccagtttgc
    55201 gcgttgccag ttcggttttc ccgggttctt tactgtcgta acgaattaaa tcaccgtcca
    55261 gtgccagctt cacgccgctg gccttactga ccgcgttctg aaacgcggca ttacggctgg
    55321 cgtaccagcc agcgttaaaa tcagcaaagc ggtatatcgg tgcgctatag ctggcgggat
    55381 aattcagtaa atggtaagta ccaaaccaca acccgccgcg gcgactgaag acttcctgac
    55441 gaactgtacc gtccattttc cacggatacc ctttggtatg ctgttcggca aaagcaatgc
    55501 tgacctgcat cggcccaccg gtgcgcacag gattaagcga accaaacagc gtctgcccca
    55561 ttggtaccat gctgatcaga tcgtcaaaaa tcgcgctcaa ttgcttttct gtacgcaccg
    55621 aatctaaccg ttcgctataa cttttgccgt ttggcgattt gatttttagc gcggtatgaa
    55681 ccagaaaagc aggaatgtgc atccgttcag cacgacggtc aatctcttgc caggcgatct
    55741 tgcttaagcc cggaacggcc ggatctgcct gataatttga ctcctgctgc gccaccgcca
    55801 gcaccgagca gacgttttcc agcgtcggtg ccagcccctg actttcaaag gtggtggcga
    55861 gatctttcgc ccaggcatcg cggtctttca cgctcgcagg catcttctgc cgcacgacgc
    55921 tcgctacatc tatcgctttt tcgccctttt tcaacggttg cggagcctga ctactacacc
    55981 ccgctaaaac cagaaccgcc aacaatgaca gcgaagatag atgattgacg cgcgacatta
    56041 cttctccttt ttagctcaag gtatgggtta cttcctgaat tttgtcacca tccagctcat
    56101 gttcaaagct gcgcagacgt ttgtagatag acatcaactc aaccagtgtg gtccatgagt
    56161 taatcaggta ctggaaagca ccgcgaacct gaccaaaaac gttggtaatc tgcgtcatca
    56221 ggccgagcgt aatcgtaccg gcaacaattg acggaaacag caagaacaaa ccgaaaacgt
    56281 tatcgacctg caaatagaga atgcgggcga tgttgaaata catatagtga aaatagaggc
    56341 gaaaatagtt tttccgtacg gcgctaaaca gctcgcgtac cgtaggcggc gtcgcgcgcg
    56401 tggcatcgtc ttcaccataa accagctctt tacggtaggc ggcctctact cgctggtttt
    56461 taaactccag ccccggcagt ttgatcccta ccactgccag caatccggtc cccatcagcg
    56521 accagacgat agcggcaatc accagaccat acggaatgtg cccgataatc ggcagctccg
    56581 gcacatgcgc ggagagcgtt accagcaccg gcagaaaggc gatcaacgtc atgatggcgt
    56641 tgataaaact gacgcccata ttctccagcg ttgaagcaaa acgcatggtg tcttcctgca
    56701 cacgctgtgc ggcaccttcg atatgacgca gttgttgcca gttcgccatg taatattcgt
    56761 tcatcgccgt acgccagcgg aacacatagt gactgacaaa gaagttgttc agtacactga
    56821 tcaccaccgc gatcagcgca atccccagaa agacgcccac ttcgcggtaa aattgttcga
    56881 tagtgacttt atgcggcgaa cttagcgcgg tttgaatcag atcatagaac ggtgcatacc
    56941 aggcgttgac ggcgaccccg acttccacca aaaaccaggt gacgaagatg atcagtgcag
    57001 taccgagtat tgaccagtat tgccaacggt gcgggctgta gataaaccag aaaaatgcaa
    57061 aaagtcctac gcaaacaatg tagtaagcgt aaaaaatcag gaaatccaac gaccagaaac
    57121 gcgcggcgct aatcgggatc tgcccggaag cgccggtgat acgcgccacc cagtcccccc
    57181 caccggcttg ccagaagata acggcaatca atgcccaaac aaaggccgag agaaaaaacg
    57241 ctcccggctt tgggaaaaaa gacttaaaca tatgcactcc tgctaacttc ttatcgtttt
    57301 aacaactgct gtgtattaac cgcatgacca ctacaaagcg tcagccaaat gaaaccgccc
    57361 attgtgaccc atagcaaggc gcttagttcg ccagcctgac cataagaatt gtttcgacgg
    57421 atttcactaa ccaaagattg tttacataag cggattaaca cgcgcaggat cacaaatgca
    57481 ggcattggtg agcaggaaaa agatcgtgtt tacgcgccgt tacgttttta acactattat
    57541 tgcactaatt cgccctttgc aatccatcaa tgagtagtat aaatacgctc agttaccttc
    57601 attcaatcta tggacaccac cgttgaaacg tagtctgctt ttttctgccg tgctgtgtgc
    57661 ggcgtcattg acctccgtcc atgcggcgca gccgatcact gaaccggagt ttgcctctga
    57721 tattgtcgat cgttatgccg atcatatttt ttacggcagc ggtgccacgg ggatggcgct
    57781 ggttgttatc gacggtaacc agcgcgtctt tcgtagttat ggcgaaacgc gacctggtaa
    57841 taacgttcgc ccgcagctgg attcggtcgt gcgtattgct tccctcacca aactaatgac
    57901 cagtgaaatg ctggtgaaat tgctcgacca ggggaccgta aagttaaacg atccgctaag
    57961 taaatatgct ccgccaggcg cacgcgtgcc gacctacaat gggacgccca ttaccctagt
    58021 gaatctggca acccatacca gcgccctgcc ccgtgaacag cccggtggcg cggcacatcg
    58081 tccggtgttt gtctggccaa cgcgcgaaca acgctggaaa tacctttcta cggcgaagct
    58141 aaaagccgcg ccaggcagtc aggcggcgta ctctaacctt gcgttcgacc tgctggcaga
    58201 tgcactggcg aatgcgtccg gcaagcctta tacccagttg tttgaagagc agattacccg
    58261 tccattgggg atgaaagaca ccacctacac cccctcaccg gatcagtgcc gccgtttaat
    58321 ggttgctgag cgcggtgcca gtccgtgcaa taacacgctg gcggcaattg gcagcggcgg
    58381 cgtctattcc acgcctggcg atatgatgcg ctggatgcag cagtatttgt catctgattt
    58441 ttatcagcgc agtaaccagg cagatcgcat gcagacgttg atttatcagc gcgcgcagtt
    58501 tacgaaagtg attggcatgg atgtccccgg caaagcggat gcgcttggtt taggctgggt
    58561 atatatggcc ccgaaagagg gtcgtccggg gattattcag aagacaggag gtggtggcgg
    58621 tttcattacc tatatggcga tgatcccgca gaagaatatt ggcgcgtttg tggtggtcac
    58681 tcgttcgccg ttgacgcgct ttaaaaatat gagcgatggc atcaatgatc tggtgaccga
    58741 gctaagcggg aataaaccgt tggttatccc cgcatcctga ttaatactct gaaggcaaac
    58801 ggttgatagc gaccagtttg cctttattca tttcgatata gccgccttta cgcaaagcag
    58861 cgagaacttc tgcgacgaca gaacgcgata tgcgtgtacg ttgatggata taattcatca
    58921 caccaatacg tgagcgcaat tcttcattcc agtcaatcat tgataataat gttgcgcgga
    58981 tttgttcgta ggaattatgc ccaatgagct gcacgtcgcg taattccaga attctatttt
    59041 gccaggctaa ccagtaaaaa gcgtctcgcc agagttgatt ttgttcaata agcgtaatgg
    59101 tttgtttggc tggtagatga tatcccgtac aatttccttc tgatattaat ttgtatggta
    59161 tatcgttttt cattaaacca tcagccagcc ccataatata aggagcctgg gtaataccga
    59221 taagtacgtt ttcttctcta cgcagagaga taacgccctc taaaataacg aaagtatctt
    59281 cgctattgac atcactggaa aatataactt gcttttcatt attaaactcg aagcgcgtac
    59341 cgtatctgga caaacattta tcgagcttac caaattcctg aagaggttta actacagata
    59401 acatttgcgc gtcctttgca gtaatgcccg tcaaatcctt gacgggcatt atttagatta
    59461 aattaccaga atttcttcgg agtgaagaat attaccaggt atatttaaca cccacgttcg
    59521 cggaccagtc ttgatctacg tcaccaccac cgaggtagtt agcatcggta taggcgctga
    59581 agttcttggt gaagctgaac tgagtaccca gaccaacacg taccgcagac ccttcagtac
    59641 cgttatcgat ggaatcaccg ttcacatcgt tatcgttgtt agagtcgtcg tagacgtaag
    59701 ccagtttgaa gtacggagtc agagcctggt cttcgctgta ggtgaaggta taacctgcat
    59761 ctacacccag ttcataacgc atgctgtcgt aagactgacc gtcaactttc atgtcgttgc
    59821 tcagctggta gtcatcacca gactggaaca gaccagaaat gctgccgtaa ggagtcacgt
    59881 aaccagcatc acccagtttg aagtcgtaac cggctttcaa accgaagccc caagcgtcgg
    59941 agttggtgct accgtcaacg taagtaccgt tgctcatggt tgcagacagg tcgttgttga
    60001 agtgagagta actcaagcta ccatcaacaa agacgttgtt cgcgaagtga gcagaagagt
    60061 agatgtaggc agtctggctg tcttgatcca cctgaccaga acggtcattc atgtcacctt
    60121 tagcgaagcc tgcagccgca ccgacgatcc acttagcgtt gttaccgtca attttggtat
    60181 caacaccgac catgatgccg ttaacatcct gatcatagtt gatggtgccg ttgtcgccgt
    60241 tgaagttacc accgaagtag cttacccatg cgccgccgtt atcagccagg ccatgacgag
    60301 agttggtcag acgagtacca acggtgtctt gttccaggtt ccagatattg gtgttcgcag
    60361 acgggatgct cagcgccatg ttagcgtagt cggtcagctc catctgttgc agaacaacgg
    60421 tgttaccgcg ctgttcagcc tgataggtgt atgcacccag gtcagctttg ttagcagcag
    60481 agaaggtcgc gttgctgttt ttgtcgttga cgtagatcag ctcgttgccg ttgtagtcag
    60541 cgatagaacc agcaccagta gcgttgtcga tacgaacttt gtagttaccg gttgcagctg
    60601 caacatggtc accgttgtta tcaacttcgt tctgaccagc ttcgatagaa gcagtgttgt
    60661 cgttatcgcc gttaccgtta atggtcaggt gaccatcaga gttcattgcg attaagccat
    60721 aaccgtagtt tgacttggta gtatcgttgg tacggtcgtt ggtcaggtct gcatcaagta
    60781 cgaagcggtt gctttcgatg ttgaacacac cagcgtgaat gttaccactg gtatcagtag
    60841 tgctggtcag gttcagagta tcaacctgca gagcattcgc cgcagtgtta ccgacatcca
    60901 gaataccgtc attagtcact ttgatgttgt tggcatacag agcagcatca cctacagccc
    60961 aaccagcaga aacttcatca gaaatggtta cggtactgtt gtcgacagtc agtgaatcag
    61021 ttgcaacatg accatcttcg ttgatgttca gcgcagaaag acctgtcaga gagatagagt
    61081 cagaaatcag cgttgaatca gcaacattaa cagttgaacc actgttgatg ctcagagtat
    61141 caatcagaga agttttggtg gtatcccact gtgaaccggt attcagggtc acattgaaca
    61201 gaccgctctg gtatacttcg ttaccgatga tgtgatcaaa ttcgctgtat tcagagtcgc
    61261 tgttttctac accaacagtt gatgaaggcc acaggctgtt agctgcgatg tcgatcagag
    61321 ttgcagttgc ttcggtgcct gtactggtat caataacaac cccgttgtca ttgtaaacag
    61381 gaccgtaagc atctggcaca ccatcatcat tggtatcaac agttaagtgc gcagacattg
    61441 ccgcaccaac ccacttgctg ccattgttca gggtcagatc caggcgatca gtaccatccc
    61501 aaccgttagt atctacatca ccgtcagcat cacgatagct atcacgacca tgcgggaaga
    61561 agttctcatc aaagttactt gagaagaata catcgcccat cagtgtagaa ttatcgaatg
    61621 ttgcagtggt ttgcattgcg ttatcagcag caggatgtgc aataacggct aatgcgatat
    61681 catctctatt ccagtcaccg ccattgtaat cgctggcatt gccggtatta ccaaaccaac
    61741 cactagtacc ttcatcagac catgaaccag aggtcacagt agagtcttta acagtgatgg
    61801 tgttgttaaa cacttcgctg ctgttaacac cagtgctaac cgtgttgtcg tcgatatctt
    61861 cccactcata accctgagtc aacgtaatac ccgcaacgtg agagttatta gtgatggaca
    61921 gatcaacttc ctgatccaga gtaatagccg taccaatgtt gtaaacgtct acaacatgag
    61981 tatcagcggc attattgtaa gtgccgttat atgtgtagtg ttcgtagttg tcgtcgatcg
    62041 ttgaattatc aacagtcagc gccagacggt catggtaata atcattcgca cgttcagcac
    62101 aatcagttgt catgcactcg gaataaatca taccgtgaat agtgctgttt ttgattgtca
    62161 gactattggc gttggtattg gtagaaatac cgtcgtccag atagtaggta gaaatcacgc
    62221 cgttaacagt agcgttatta atgaccgggt agatatcgcc gttgtaataa ctatcagcgg
    62281 catagttgtt ccagcctaca taaccatcat agtaaacgtc atcagcatag gtggcgtcgt
    62341 tgtagtgatg gaaagtattg taagtttgac cagaaatatc gttggtcgct gcattagcct
    62401 gagaggtgat agccagagtg caagccaatg ctaattgtga tactacaagt ttctttttcc
    62461 aggagtgcat ttgtcatccc tcctcaggga cgtaataagt tgatccatca attatcaatg
    62521 catagaaaac atattatgtt tcgcgctaac gattatgctg cgttacagac gaatggttca
    62581 attattcttt cccctaagtc cgaatgtgga catatataat ttatggagaa aaacacgcat
    62641 caaattgata aaaggacgat atttcaacat gttgcagata atgttatttt tgatacatac
    62701 gcgttataaa tatcaaaaac acaaaccaga taaatatctt atcaatatac aacaaagttt
    62761 gaattattca atttatagca gcacaaccaa aggccaaaaa aaataataac caagagctat
    62821 aaataaagat taaaaatatt ttcagtgaat atgaaattat tgctatttct attacaaaga
    62881 tgtcctggtc tctctttcat tctcttcttg tcaacatcac gacaactttc gtaaaacact
    62941 cctgccccgc ttcaggtata ctgtgcacct taattccaca aacatcaggc agaccatgac
    63001 agacttaatc caacgccctc gtcgcctgcg caaatctcct gcgctgcgcg ctatgtttga
    63061 agagacaaca cttagcctta acgacctggt gttgccgatc tttgttgaag aagaaattga
    63121 cgactacaaa gccgttgaag ccatgccagg cgtgatgcgc attccagaga aacatctggc
    63181 acgcgaaatt gaacgcatcg ccaacgccgg tatccgttcc gtaatgactt ttggcatctc
    63241 tcaccatacc gatgaaaccg gcagcgacgc ctggcgggaa gatggactgg tggcgcgtat
    63301 gtcgcgcatc tgcaagcaga ctgtgccaga aatgatcgtc atgtcagaca cttgtttctg
    63361 tgaatacact tctcacggtc actgcggtgt gctgtgcgag catggcgtcg acaacgacgc
    63421 gactctggaa aatttaggca agcaagccgt ggttgcagct gctgcaggcg cagacttcat
    63481 cgccccttcc gccgcgatgg acggccaggt acaggcgatt cgtcaggcgc tggacgctgc
    63541 aggctttaaa gatacggcga ttatgtcgta ttcgaccaag ttcgcctctt ccttttatgg
    63601 tccgttccgt gaagctgccg gaagcgcatt aaaaggcgac cgcaaaagct atcagatgaa
    63661 cccaatgaac cgtcgcgagg cgattcgtga atcactgctg gatgaagccc agggcgcaga
    63721 ctgcctgatg gttaaacctg ctggagcata cctcgacatc gtgcgtgagc tgcgtgaacg
    63781 tactgaattg ccgattggcg cgtatcaggt gagcggtgag tacgcgatga ttaagttcgc
    63841 cgcgctggcg ggtgctatag atgaagagaa agtcgtgctc gaaagcttag gttcaattaa
    63901 gcgtgcgggt gcggatctga ttttcagcta ctttgcgctg gatttggctg agaagaagat
    63961 tctgcgttaa tttttttcct ctcagaaagg gggagttaac ctgttgaaag tacggtgaac
    64021 gccccctcac cctaaccctc tccccaaggg ggcgagggaa ccgtccgcac tcgtatcacc
    64081 ccgcccgata aaacggttta tccccaagga tcgtcgcccg atgcataatc cgccgctgtg
    64141 gcaggtaatc ggcattggca tagtgctgag tcacgcggtt atcccaaatc gcaatatcat
    64201 tcggttgcca gcgccaacgc acctgaaact ccggtttagt gatatgggcg aataaaaagc
    64261 ctaacaaggc ttcgctctct ttctcgctga catcgacaat tcgcgtagta aagccttcat
    64321 tcacaaacag cgcctgttta ccgctcaccg gatgcgttcg caccaccgga tgtagcaacg
    64381 gaggattttt cgcgaccgcc tcgcgccagc gttgatgttc ctcctcggtt ttgcggtatt
    64441 tgtattccgg gaacgattta cggaaatcat gctccgcacg cagcccgctc agcagctggc
    64501 ggaagggaac agagagcgcc tcataggccg caataccgct ggtccagagc gtatcaccgc
    64561 cggtcgaagg taactcttta gctgccagaa tcgcccctgc gggtggcgtt tcaataaatg
    64621 tcacatcggt atgccagttg tcgttatccg gcggattatc gttatgggta tccagcacga
    64681 tgatctcgtc aaccccttcg gcatgagggt aaacagggtg aatatgcagt tcgccaaaac
    64741 gctgggccag cgcgcgttgc tgctgcggcg taatagtttg atcgcgcaga aacaccacct
    64801 gatggcgcag caccgcatgg taaagctgtt caaactgatt atcgcttaac gggcgcgtca
    64861 ggtcggcacc cgaaatttgt gcgccgatat acggccccag cggggtaatg ctcagacgtt
    64921 cactcattgt acttctccat gccagggcgt cagacggcgc tgtaacgcgc gcaaacccag
    64981 ttctaaaaga aaggcgataa tcgcaatcac cgcgattccc gccagcacca cgtcagtcgc
    65041 gagaaattca cccgctgact gaaccataaa tcctaaaccg cgcgtcgcgg caatcagctc
    65101 cgccgctacc agcgtagacc agcccacacc cagaccaatg cgtaatccgg tgaggatttc
    65161 cggcagcgcg ccgggcaaaa tgacaaacca cagcacctgc gcacggctgg cacccagcga
    65221 ctgggcggca cgaatgcgaa cctgctgcgc gcttttcacc cccgccagcg ccgacatcgc
    65281 caccggtgca aaaatcgcta aatagatcag taagatcttc gaggtttcac caataccaaa
    65341 ccagatcacc atcagcggca aataagccag cggcggcacg ggacgataaa gttcgattac
    65401 cggatccaga atgccgcgca ccgtcgggct tagccccatg gcgatcccga ccggaatacc
    65461 gatcaccacc gccgcgagta gcgccagcac aatgcgcgtc aggctggctg ccagatgctg
    65521 ccacagcgtg gcgtccataa atccctgcgg tccggcaatg gtgagcagtt ttgccagtac
    65581 ttgttgcggc ggcggcaaaa acagcgggct aatcagttgc agcgccgcca ccgtccacca
    65641 tacagtgagt aaaaccgcta acgtgccaat gcttaaggtc acctgacgcg agagcggcca
    65701 gcgccatttc agccgccgcg aatgcagttt ttcattaatg agcacactca tgagaacgcc
    65761 tcccgttgct caaatacgcg gcttaaaaca tattcgcgca tggcgatgaa ttgtggatcg
    65821 gacttgatgc tgcggctcga ctctcccgca acaaagcggc gagcaaagtt gagcggcagc
    65881 cgctccagca cacggccagg gccggatgaa agcagaacca gttcagtcgc cataaacacc
    65941 gcttcttcta tatcgtgggt aatcaacagc acctgcttgc ccgtctcctg ccagagtttc
    66001 agcagcagag tttgcatctg gtcgcgggtg aaggcgtcca gcgcaccaaa cggttcgtcg
    66061 agtaataaca gctggggatt cgccgccagc gcgcgggcaa tccccacccg ctgacgttga
    66121 ccaccggaaa gctgccagat atagcgtttt tctgcgcctt ccagccccac ttttttcagc
    66181 atctggtgcg cgatttccag tcgctgcatt ttctctatac ctgccaattg caggccgaac
    66241 gccacgttgt cctgtacatt gcgccacggt agtagccctt cattctgaaa aaccaccccg
    66301 cgctctgctc ccggtccctc aatacgctta cccgccagtt gaatgctgcc atgctgataa
    66361 ggcacaaaac cggcaatcag attcagcagg gtggttttac cgcagccgga cggccccagc
    66421 accaccagta gctcgccgct ttccagcgtc aggttgatat cttccagtgc cggtttgccg
    66481 ccataatcgg catagagatg agagatttgc agcatctgcg cctcctttta ttgcacgaag
    66541 cgcgaggtaa cgtactggct gtaatcattc gctacagccg ggaccttgcc ctgctctttc
    66601 aaaaactgcg cggtgtcgat gattgctttg ttcaccggtc cggtcagttc tgccgtttgt
    66661 tgctgcggcg tcagataggt atttcccttc accagccccg gaacgtcacc ttcaggcacg
    66721 ccgcttaaac gcgccagttt gctgatgttt tccggctgtt tcagccacgc gtctgggtta
    66781 gcaatgtacg gttgctgagc atcgatggcg cttttagcga acgctttcac gacctcagga
    66841 tgtttctcgg caaaatcttt gcgcaccacc cagacgtcca gcgttggcgc accccactgc
    66901 ccgacctgtt cagaatcggt caacaccttg ccgtcttttt ccagggcgtt aaccgccggt
    66961 gcccagacat aagcaccatc aatatctccc cgctgccagg cagcgataat cgcgggcggc
    67021 tgcaggttca caatctccac ttgcccgggt ttaatgcccc agtgtttcag cgccgccagc
    67081 aggctgtagt gggtggtaga gataaacggt acggctatgc gtttgccaat cagatcttcc
    67141 ggtttgctga tagttttctt taccaccagc gcttcggagt tacccagttt tgacgccagc
    67201 aagaaaactt caatcggcac ctgttggctg gctgcaaccg ctaacgggct ggaaccgagg
    67261 ttgccgattt gcacatcgcc agaagccagc gcccgcacga tgctggctcc gctgtcaaac
    67321 ttacgccagt ccacggttgc tccgctttct ttagcaaagg tgttgtcggc ctgagccact
    67381 ttcgccggtt cggctgaggt ttgatacgcc acggtgacgt tcaccgcctg tgcctgaaaa
    67441 gcgatgaatg ccagtgcggc aagaagtgtg ttacgcgatg aaattgccat gattgtctgc
    67501 tcccctgtct tgttatggga gcagtattca ggaataaaaa cattcattaa aagaattagt
    67561 cgttatcgca cagatgattt tattcttagc aaaaaaacgg ctaaggaaag attaaatatg
    67621 caattactcc gttctaacaa ttgggaaaat tactacactt cattttagtg atgcataaac
    67681 aaccacatta cgagatttat tacttcatta tttctatcaa tatcaataca tataataaaa
    67741 ctattatatt cgcattgtat atatcactgt atcattctc
//
LOCUS       DAZGXZ010000009        64692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000009 DAZGXZ010000000
VERSION     DAZGXZ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.009"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(163..1143)
                     /gene="ygfZ"
                     /locus_tag="ACHKJF_000693"
     CDS             complement(163..1143)
                     /gene="ygfZ"
                     /locus_tag="ACHKJF_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP0948111.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1386..1652
                     /gene="sdhE"
                     /locus_tag="ACHKJF_000694"
     CDS             1386..1652
                     /gene="sdhE"
                     /locus_tag="ACHKJF_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP0948112.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            1633..2040
                     /gene="ygfX"
                     /locus_tag="ACHKJF_000695"
     CDS             1633..2040
                     /gene="ygfX"
                     /locus_tag="ACHKJF_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP0948113.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2080..2601)
                     /gene="fldB"
                     /locus_tag="ACHKJF_000696"
     CDS             complement(2080..2601)
                     /gene="fldB"
                     /locus_tag="ACHKJF_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP0948114.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2713..3609
                     /gene="xerD"
                     /locus_tag="ACHKJF_000697"
     CDS             2713..3609
                     /gene="xerD"
                     /locus_tag="ACHKJF_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP0948115.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3634..4344
                     /gene="dsbC"
                     /locus_tag="ACHKJF_000698"
     CDS             3634..4344
                     /gene="dsbC"
                     /locus_tag="ACHKJF_000698"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP0948116.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            4350..6083
                     /gene="recJ"
                     /locus_tag="ACHKJF_000699"
     CDS             4350..6083
                     /gene="recJ"
                     /locus_tag="ACHKJF_000699"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP0948117.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMILKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6174..7272
                     /gene="prfB"
                     /locus_tag="ACHKJF_000700"
     CDS             join(6174..6248,6250..7272)
                     /gene="prfB"
                     /locus_tag="ACHKJF_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP0948118.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7282..8799
                     /gene="lysS"
                     /locus_tag="ACHKJF_000701"
     CDS             7282..8799
                     /gene="lysS"
                     /locus_tag="ACHKJF_000701"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0948119.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8842..9390)
                     /gene="idi"
                     /locus_tag="ACHKJF_000702"
     CDS             complement(8842..9390)
                     /gene="idi"
                     /locus_tag="ACHKJF_000702"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP0948120.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            9445..9516
                     /locus_tag="ACHKJF_000703"
     CDS             9445..9516
                     /locus_tag="ACHKJF_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948121.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(9513..9638)
                     /gene="yqfG"
                     /locus_tag="ACHKJF_000704"
     CDS             complement(9513..9638)
                     /gene="yqfG"
                     /locus_tag="ACHKJF_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP0948122.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(9640..11088)
                     /gene="uacT"
                     /locus_tag="ACHKJF_000705"
     CDS             complement(9640..11088)
                     /gene="uacT"
                     /locus_tag="ACHKJF_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP0948123.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            11524..13443
                     /gene="ygfT"
                     /locus_tag="ACHKJF_000706"
     CDS             11524..13443
                     /gene="ygfT"
                     /locus_tag="ACHKJF_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP0948124.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDCLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTQAS"
     gene            13443..13931
                     /gene="ygfS"
                     /locus_tag="ACHKJF_000707"
     CDS             13443..13931
                     /gene="ygfS"
                     /locus_tag="ACHKJF_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0948125.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(13967..15334)
                     /gene="ghxQ"
                     /locus_tag="ACHKJF_000708"
                     /gene_synonym="ygfQ"
     CDS             complement(13967..15334)
                     /gene="ghxQ"
                     /locus_tag="ACHKJF_000708"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP0948126.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(15370..16686)
                     /gene="guaD"
                     /locus_tag="ACHKJF_000709"
     CDS             complement(15370..16686)
                     /gene="guaD"
                     /locus_tag="ACHKJF_000709"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP0948127.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(16704..18104)
                     /gene="xanQ"
                     /locus_tag="ACHKJF_000710"
     CDS             complement(16704..18104)
                     /gene="xanQ"
                     /locus_tag="ACHKJF_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP0948128.1"
                     /translation="MSDINHAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEEVIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(18269..21139)
                     /gene="xdhD"
                     /locus_tag="ACHKJF_000711"
     CDS             complement(18269..21139)
                     /gene="xdhD"
                     /locus_tag="ACHKJF_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP0948129.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(21136..21915)
                     /gene="ygfM"
                     /locus_tag="ACHKJF_000712"
     CDS             complement(21136..21915)
                     /gene="ygfM"
                     /locus_tag="ACHKJF_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP0948130.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(21966..23294)
                     /gene="ssnA"
                     /locus_tag="ACHKJF_000713"
     CDS             complement(21966..23294)
                     /gene="ssnA"
                     /locus_tag="ACHKJF_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP0948131.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVN
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(23297..26395)
                     /gene="ygfK"
                     /locus_tag="ACHKJF_000714"
     CDS             complement(23297..26395)
                     /gene="ygfK"
                     /locus_tag="ACHKJF_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP0948132.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGYPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(26717..27295)
                     /gene="mocA"
                     /locus_tag="ACHKJF_000715"
     CDS             complement(26717..27295)
                     /gene="mocA"
                     /locus_tag="ACHKJF_000715"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP0948133.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            27398..28168
                     /gene="yqeC"
                     /locus_tag="ACHKJF_000716"
     CDS             27398..28168
                     /gene="yqeC"
                     /locus_tag="ACHKJF_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP0948134.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            28216..29841
                     /gene="yqeB"
                     /locus_tag="ACHKJF_000717"
     CDS             28216..29841
                     /gene="yqeB"
                     /locus_tag="ACHKJF_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP0948135.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(29882..30814)
                     /gene="arcC"
                     /locus_tag="ACHKJF_000718"
     CDS             complement(29882..30814)
                     /gene="arcC"
                     /locus_tag="ACHKJF_000718"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0948136.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(30862..32247)
                     /gene="hyuA"
                     /locus_tag="ACHKJF_000719"
     CDS             complement(30862..32247)
                     /gene="hyuA"
                     /locus_tag="ACHKJF_000719"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP0948137.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(32300..33511)
                     /gene="ygeY"
                     /locus_tag="ACHKJF_000720"
     CDS             complement(32300..33511)
                     /gene="ygeY"
                     /locus_tag="ACHKJF_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP0948138.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGIE
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(33569..34765)
                     /gene="dpaL"
                     /locus_tag="ACHKJF_000721"
     CDS             complement(33569..34765)
                     /gene="dpaL"
                     /locus_tag="ACHKJF_000721"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP0948139.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(34823..36010)
                     /gene="ygeW"
                     /locus_tag="ACHKJF_000722"
     CDS             complement(34823..36010)
                     /gene="ygeW"
                     /locus_tag="ACHKJF_000722"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP0948140.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQAPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            36489..38267
                     /gene="ygeV"
                     /locus_tag="ACHKJF_000723"
     CDS             36489..38267
                     /gene="ygeV"
                     /locus_tag="ACHKJF_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP0948141.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(38307..38786)
                     /gene="xdhC"
                     /locus_tag="ACHKJF_000724"
     CDS             complement(38307..38786)
                     /gene="xdhC"
                     /locus_tag="ACHKJF_000724"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP0948142.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(38783..39661)
                     /gene="xdhB"
                     /locus_tag="ACHKJF_000725"
     CDS             complement(38783..39661)
                     /gene="xdhB"
                     /locus_tag="ACHKJF_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP0948143.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(39672..41969)
                     /gene="xdhA"
                     /locus_tag="ACHKJF_000726"
     CDS             complement(39672..41969)
                     /gene="xdhA"
                     /locus_tag="ACHKJF_000726"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP0948144.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            42384..43139
                     /gene="actS"
                     /locus_tag="ACHKJF_000727"
     CDS             42384..43139
                     /gene="actS"
                     /locus_tag="ACHKJF_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP0948145.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            43218..43291
                     /locus_tag="ACHKJF_000728"
     tRNA            43218..43291
                     /locus_tag="ACHKJF_000728"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43250..43252,aa:Gly,seq:ccc)
     gene            43456..44670
                     /locus_tag="ACHKJF_000729"
     CDS             43456..44670
                     /locus_tag="ACHKJF_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0948146.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLVVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGVCA"
     gene            45214..45417
                     /locus_tag="ACHKJF_000730"
     CDS             45214..45417
                     /locus_tag="ACHKJF_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0948147.1"
                     /translation="MANNNSFIRLSEVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSV
                     AFIESEVDEWINQRIDASRNNAA"
     gene            complement(45997..46233)
                     /locus_tag="ACHKJF_000731"
     CDS             complement(45997..46233)
                     /locus_tag="ACHKJF_000731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948148.1"
                     /translation="MNKKYQSITDIFAEQRRQLLHRILERQLELKRAKEISEPEELTP
                     LPGVKKHPQKRFALEDFVEKYPRRLKATKNRPPG"
     gene            complement(46291..46470)
                     /locus_tag="ACHKJF_000732"
     CDS             complement(46291..46470)
                     /locus_tag="ACHKJF_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0948149.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKESE"
     gene            46667..46867
                     /locus_tag="ACHKJF_000733"
     CDS             46667..46867
                     /locus_tag="ACHKJF_000733"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0948150.1"
                     /translation="MRNHITHPQGRDSHNLNKFTWRFMQCLTDSIAIFTVTAATEHEA
                     RAQLPHAHLIFVARIRQEVTHA"
     gene            46860..47045
                     /locus_tag="ACHKJF_000734"
     CDS             46860..47045
                     /locus_tag="ACHKJF_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948151.1"
                     /translation="MLKTFRVFSRAVNKRGYTVGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVHEIKGVQ"
     gene            47045..47248
                     /locus_tag="ACHKJF_000735"
     CDS             47045..47248
                     /locus_tag="ACHKJF_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948152.1"
                     /translation="MQEMTLAEASLKAQQIEVISRMIERMIERNHEEIDDHDLSVIAG
                     LIKQLSSNVAVWLMTEEEKRGEQ"
     gene            47306..47601
                     /locus_tag="ACHKJF_000736"
                     /pseudo
     CDS             47306..47601
                     /locus_tag="ACHKJF_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            47598..49736
                     /locus_tag="ACHKJF_000737"
     CDS             47598..49736
                     /locus_tag="ACHKJF_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP0948153.1"
                     /translation="MKKAPNLKLQPKDKMTEVIIFAGSDAWAHAKEWSEWAGKHIAAD
                     DTPPVILGPKQLASLEDTKIIDKGRNYVRVYRAGEISETALLQIATLLAVAGVKEARC
                     YSGFVDQQPEDWTPRMAGLKDDAERGNSLVINLPAKTNFTGNYDDELKPRVECRPDGV
                     YWVTPKVDKQSGEIIRPETWLCASVELLGAGVIGNEHYRVMRWTDSTTNRLVTMAVPC
                     CGIGDSDGWRLLKANGLKVTTNGKHRIPLADWMQLGGQHEEWHLSTKAGWHFGAYIMP
                     DGTVIGESDKPVLFTGKSAAINGYSVAGTADSWRDSVARLAGGNPFMMLGIATSLSAP
                     LVGLVGADGFGVHFFENSTAGKTTTQSVASSLWGDPEAQRLTWYGTALGIANEAEAHN
                     HGLLPLDEIGQAASARDVYVSAYTLFNGFGKLQGAKDGGNRELKNWRAVAISTGEVDI
                     ETFLKTEGIKVKVGQLVRLLNVPMEKATKFHEYSNGKEHADALKEAWKENHGAAGREW
                     VKWLSGHQQEAKDTVRECRERWRNLIPESYGEQVHRVGERFAILEAALVLSGHVTGWA
                     VQECRDAILHNFNAWVKVFGTGNKEHKQIIEQAEAFLAAYGMSRFAPVNYDPASLPIP
                     ELYGYRESDGRYDEPVLFYVLPDPFGSHVANGFNKDAAAKVLHEAGMLKRPSSGRGWQ
                     IRTPRLKHLKGARLRVYGLLLAQDHDTESD"
     gene            50097..50426
                     /locus_tag="ACHKJF_000738"
     CDS             50097..50426
                     /locus_tag="ACHKJF_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948154.1"
                     /translation="MSEITVKNISPAVAGWWAKFRDDDGTEWYSPIAAWALCEVDYFG
                     AGNTRREILPVLTSELGMGPHPSDEGYCACLYLPDEKFTLSGEQGSFAWYPVNSNSNN
                     GNSGAES"
     gene            50423..50842
                     /locus_tag="ACHKJF_000739"
     CDS             50423..50842
                     /locus_tag="ACHKJF_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948155.1"
                     /translation="MTAKHTKNHNSVVKFVSARGRPTLLVPGKHLHYCDENHIPIVIV
                     WKRTVYADVTWLYDSLALMHRDLFEREEFRLNIEERAEKIYEQYAANSKRAARAITHH
                     FMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNRERRP"
     gene            50839..51288
                     /locus_tag="ACHKJF_000740"
     CDS             50839..51288
                     /locus_tag="ACHKJF_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0948156.1"
                     /translation="MTAQIAAYGRLVDDPQEKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALDRAKRQTGQQGNQPPAGGDDPYGDDIPF"
     gene            51456..51752
                     /locus_tag="ACHKJF_000741"
     CDS             51456..51752
                     /locus_tag="ACHKJF_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP0948157.1"
                     /translation="MKPLKTGVIRDVLKDAKERELPIGHGVLRGALISYIHTTRYLNA
                     VIAGGARYDLNGQPCGEVTEEDKSVASELLKRRLAQIKQQNSQQQTTGDDGDDS"
     gene            51742..52035
                     /locus_tag="ACHKJF_000742"
     CDS             51742..52035
                     /locus_tag="ACHKJF_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0948158.1"
                     /translation="MIADSVAQKLEERGLWRRAATRWTEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLAEMGINPQDEWRNYSFSDAGDDLALP"
     gene            52861..53100
                     /locus_tag="ACHKJF_000743"
     CDS             52861..53100
                     /locus_tag="ACHKJF_000743"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948159.1"
                     /translation="MRKKLRKKVRKSIIKPITKSITQTNRAKPDMTRLAGGKATGKSA
                     QKNAQNETQQPRKTKPIRDDRKSAQIYRIRVSNFA"
     gene            53169..53330
                     /locus_tag="ACHKJF_000744"
     CDS             53169..53330
                     /locus_tag="ACHKJF_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948160.1"
                     /translation="MTERSPTEIIHGIIEEQEERELILMIEEQRERQRKQEATQPDSV
                     SAENQADVT"
     gene            53828..54220
                     /locus_tag="ACHKJF_000745"
     CDS             53828..54220
                     /locus_tag="ACHKJF_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0948161.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            complement(55052..55426)
                     /locus_tag="ACHKJF_000746"
     CDS             complement(55052..55426)
                     /locus_tag="ACHKJF_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948162.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDLTVVDAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            56034..57632
                     /locus_tag="ACHKJF_000747"
     CDS             56034..57632
                     /locus_tag="ACHKJF_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0948163.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKK"
     gene            57675..58283
                     /locus_tag="ACHKJF_000748"
     CDS             57675..58283
                     /locus_tag="ACHKJF_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948164.1"
                     /translation="MVKEFLVSKENKPSYECMNTAETCLGLAGMASEDWQREHHCRTG
                     IIFTAFAIEAMFIFYRKQVDPGYDKTQKECRKTMHKNTLKLCGINNYMGTKPYQIIKE
                     CLEVRDAIAHGDSYTSSFNFSADHLDNQDDIAKSVLAVNSEQFRGLTVERLAKYIEMA
                     KHIDHEISDNGYRPSEGYLPAAERRSLMLAFGVSGVSMWPSA"
     gene            59116..59616
                     /locus_tag="ACHKJF_000749"
     CDS             59116..59616
                     /locus_tag="ACHKJF_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948165.1"
                     /translation="MSIKLTTMQAAYLTRAMTTGGGLIELGELAKGLGKQKAHVVRKL
                     EANFDKERLSKMETHLTVMGGNHKNVAQTVKTYMLDYKTAGALAMSYDLQLGVEVLTL
                     LEEAVQTIGAMTGAVQAGDTQAALEASLLFSERMRNRLEYTSGETENETRSVALKRLG
                     RKGASV"
     gene            59613..59798
                     /locus_tag="ACHKJF_000750"
     CDS             59613..59798
                     /locus_tag="ACHKJF_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948166.1"
                     /translation="MIDPRTPEGRLTLRYRGLRTSLLLSMLGLDKDATDNRPFYSRNE
                     LIERLVIRDMEFNRGNK"
     gene            complement(59779..60087)
                     /locus_tag="ACHKJF_000751"
     CDS             complement(59779..60087)
                     /locus_tag="ACHKJF_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948167.1"
                     /translation="MPKLAISLLLYLACFYAVGYGAGYITGGIAWHTLYMLERIITIA
                     LLAFIPVFIFAVLIKASKEQRIIKSLSVAAINIIPSAIALLVGFTDAVTRGFLTYFLG
                     "
     gene            60310..60801
                     /locus_tag="ACHKJF_000752"
     CDS             60310..60801
                     /locus_tag="ACHKJF_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948168.1"
                     /translation="MLFNNNDWKLSVTDINPYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQQNKRVEAERIAKEQRIQAD
                     RMREENMTDEEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            60885..61874
                     /locus_tag="ACHKJF_000753"
     CDS             60885..61874
                     /locus_tag="ACHKJF_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948169.1"
                     /translation="MNMTKNAFQFGIEPVHITDNDNLQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDTLYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            62548..64157
                     /locus_tag="ACHKJF_000754"
                     /pseudo
     CDS             62548..64157
                     /locus_tag="ACHKJF_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            64170..64289
                     /locus_tag="ACHKJF_000755"
                     /pseudo
     CDS             64170..64289
                     /locus_tag="ACHKJF_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            64249..64692
                     /locus_tag="ACHKJF_000756"
                     /pseudo
     CDS             64249..64692
                     /locus_tag="ACHKJF_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT        16640 a        15647 c        16753 g        15652 t
ORIGIN      
        1 cagcgtcgca tcaggcatcg gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat
       61 ctacgggcct cgtccctgtt gtgataaagg catcaaatcc cagacatgat aagacgcgtc
      121 agcgtcgcat caggcgataa atgcctgatg cgatacagag atttactctt cgagcgaata
      181 cggcagcggc tcgatatgca gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg
      241 ttccatatcg ttattcatga cgacctgtac cacgacctga ccatcttcca gttttaccgc
      301 agccagcacc gtaccggtac gacgccagtt ctcgcccatt ttcagctcta agtcttcacc
      361 agcttccggc agtcggctgg cgctacctgc cagcaaccag agcgcacgtt tattggcacc
      421 acggaatttt gctcgcgcca ccatctcttg tccggtataa cagcctttct taaagctgat
      481 accgcccagc gcctggagat tggtcgcctg tgggataaac tgcccgctgt tggcggcatc
      541 aatcaccggg aaacccgctt caatgtttaa tgccagccac tgttggctat tgttcagttc
      601 cgcttcaccg cgcagtttat cggtcagcat attagcagta gcttcatcgg ttacgatcag
      661 gaaacgttct gccgggtgtt caaaccatag caaagtggtc gcgccttctt tgactacctg
      721 tttttctttc gaaggcagtt cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc
      781 ggcaacacca agcagcacac gctcgtcgtc tggcgcgatg gtcactttag agaataccgc
      841 atattttttc agttcagtca gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa
      901 gccatcgccg tcgcggaaca gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg
      961 ggcggcgagc aggtgctgat cttctgccat ctggctgaca tctgctgtta cctgaccctg
     1021 catatatttt tcgctgtccg cgccagtaat ggtggcaagc gcccagtcat caagcgtcat
     1081 cagcgtcagc ggtaaacggg cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc
     1141 cataatagag tcctgagttg cttaacggcg tattaatgac taatggtaaa agagctatga
     1201 ggcaatgcaa gtgctttcac tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa
     1261 tgaagccatt gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc
     1321 cgtaaaagaa ggcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga
     1381 agaagatgga cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg
     1441 aactcgatat ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg
     1501 aaaaacgcat ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga
     1561 tgaatcacgg taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac
     1621 ggaaccggga acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg
     1681 ctttccttgc tgattcatgg gctggttgcc gctgttattt tactcatgcc ctggccactc
     1741 agttacaccc cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc
     1801 cagcggcgta ttaatgctcg ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt
     1861 tggcaagggc aggagtggag catcgtcaaa gcaccgtgga tgattaagag cggcatgatg
     1921 ctgcgtttac gttctgatgg cggtaaacgg caacatttat ggctggcagc cgacagcatg
     1981 gacgaagccg aatggcggga tttacggcgg attttgttgc aacaagagac gcaaagataa
     2041 agatgacggc aaagcatatg cgctaagccg tcagtgagat caggcgtaat gctctgccat
     2101 ttcgttgagg atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt
     2161 agtttcatcc agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct
     2221 ggtaaattca tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag
     2281 tttgtcatgc agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc
     2341 aagcccatac agcgcaacaa ttttaccttc aaggttcagg tcgtcgagct gatcccagac
     2401 ggcttcccag tcttcctgga tttcaccaaa atcccaggtc gggataccca gaatgagcac
     2461 atcgtactgc tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc
     2521 tgggccgata atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc
     2581 gtaaaaaaga cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcggcta
     2641 gtgtatcaga atcgctttag tcacggcaca ataaggcata atggatcaaa tgagtgacca
     2701 taaggggcgc aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg
     2761 ctggaaaaaa atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg
     2821 gtggagtggt tgcatcaccg cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag
     2881 gcattactgg cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg
     2941 agtgcagtgc gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agacgatccc
     3001 agtgcgcatc tcgcttcacc gaaattgccc cagcgtttgc caaaagattt aagcgaagcg
     3061 caggttgaac gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa
     3121 gccatgcttg aagtgttgta tgctaccggg ctgcgtgtct ctgaactggt cggactgaca
     3181 atgagtgata tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag
     3241 cgcctggtac cgttaggtga agaggcggtt tactggctgg aaacctatct ggaacatggg
     3301 cgtccgtggc tgctgaatgg tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag
     3361 cagatgacgc gacagacctt ctggcaccgc attaaacatt atgctgtgct ggcgggtatc
     3421 gacagcgaaa agctgtcacc gcatgtgttg cgtcacgctt ttgccaccca tttattaaat
     3481 catggtgcgg atttacgcgt ggtgcagatg ctactgggcc acagcgacct ctccaccacg
     3541 caaatttata cgcatgtcgc taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg
     3601 cgggcgtgat gtctgaaaag aacgggaaga tttatgaaga aaggttttat gttgtttact
     3661 ttgttagcgg cgttttcagg ttttgctcag gctgatgacg cggcaattca acaaacgtta
     3721 gccaaaatgg gcatcaaaag cagcgatatt cagcccgcgc ccgtagctgg catgaagaca
     3781 gttctgacta acagcggcgt gttgtacatc accgatgatg gtaaacatat cattcagggg
     3841 ccaatgtatg acgttagcgg cacggctccg gtcaatgtca ccaataagat gctgttaaag
     3901 cagttgaatg cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc
     3961 atcaccgtgt ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca
     4021 gactataacg cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac
     4081 agcgatgcag agaaagaaat gaaagctatc tggtgtgcga aagataaaaa caaagcgttt
     4141 gatgatgtga tggcaggtaa aagcgtcgca ccagccagct gcgacgtgga tattgccgac
     4201 cattacgcac ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat
     4261 ggcacacttg ttccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac
     4321 caaaaaatga ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg
     4381 aagtcgatga aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg
     4441 ccagccgggg cgtgcgcagt gcgcaagaac tggaacgcag tgttaaaggt atgttgccct
     4501 ggcagcaact gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag
     4561 gaacgcggat tattgtggtc ggcgattttg acgccgacgg cgcgaccagc acggctctaa
     4621 gcgtgctggc gatgcgctcg cttggttgca gcaatatcga ctatctggta ccaaaccgtt
     4681 tcgaagacgg ttacggctta agcccggaag tagtcgatca ggcccatgcc cgtggcgcgc
     4741 agttaattgt cacggtggat aacggtattt cctcccatgc gggcgttgaa cacgctcgct
     4801 cgttgggcat tccggttatt gttaccgatc accatttgcc gggcgaaaca ttacccgcag
     4861 cggaagcgat cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag
     4921 gcgtgggtgt ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct
     4981 ggtttgatga gcgcggcatc gcaattccta acctggcaga actgctggat ctggtagcgc
     5041 tggggacagt agcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg
     5101 ggatgagtcg catccgtgcc ggaaagtgcc gtccggggat taaagcgctg ctggaagtgg
     5161 caaaccgtga tgcacaaaaa ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc
     5221 tcaatgctgc cggacgactg gacgatatgt ccgtcggtgt ggcgctcttg ctgtgcgaca
     5281 acatcggcga agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag
     5341 agatcgaaca aggaatgcaa gttgaagccc tgaccctgtg cgagaaactg gagcgaagtc
     5401 gcgacacgct acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg
     5461 gtattctggc ttcgcgcatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag
     5521 caggtgatgg tacgctgaaa ggttcaggtc gctccattca ggggctgcat atgcgtgatg
     5581 cactggagcg attagacaca ctctaccctg gcatgatact gaagtttggc ggtcatgcga
     5641 tggcggcggg tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg
     5701 aactggttac agagtggctg gacccttcgc tattgcaagg cgaagtggtg tcagacggtc
     5761 cgttaagccc ggccgaaatg accatggaag tggcgcagtt gctgcgcgat gctggcccgt
     5821 gggggcagat gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc
     5881 tggtgggcga acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccgctgctgg
     5941 atggtattgc ttttaatgtc gataccgccc tctggccgga taacggcgtg cgcgaagtgc
     6001 aactggctta caagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca
     6061 tcgacaatat ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta
     6121 caatcccgct cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg
     6181 aaattaatcc ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg
     6241 ggtatctttg actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa
     6301 cagccggatg tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc
     6361 ctcgaagccg ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt
     6421 ctgctggaac tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa
     6481 ctcgacgccc tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa
     6541 tatgacagcg ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag
     6601 gactgggcga gcatgcttga gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa
     6661 actgaaatca tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa
     6721 atctccggcg attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg
     6781 cgtaaaagcc cgtttgactc cggcggtcgt cgccacacat cgttcagctc tgcgtttgtt
     6841 tacccggaag ttgatgatga tattgatatc gaaatcaacc cggcggatct gcgcattgac
     6901 gtttatcgcg cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt
     6961 attacccaca tcccaaccgg gatcgtgacc caatgccaga acgaccgttc ccagcacaag
     7021 aacaaagacc aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag
     7081 aaaaatgccg agaaacaggc gatggaagat aacaaatccg acatcggctg gggcagccag
     7141 attcgttctt atgtccttga tgactcccgc attaaagatc tgcgtaccgg ggtagaaacc
     7201 cgcaacacgc aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa
     7261 gcagggttat gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc
     7321 gatcttaaca atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg cgagcagggg
     7381 attgccttcc cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc
     7441 gacggtaaag agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc
     7501 atgatgaccc gccgtattat gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc
     7561 cgcattcagc tgtacgttgc ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc
     7621 aaaaaatggg accttggcga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc
     7681 ggcgaactgt ctatccactg taccgagctg cgtctgctga ccaaagcact gcgtccgctg
     7741 ccggataaat tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatctggat
     7801 ctcatctcta acgatgaatc ccgtaacacc tttaaagtac gctcgcagat cctctctggt
     7861 attcgccagt tcatggtgaa ccgcggcttt atggaagttg aaacgccgat gatgcaggtg
     7921 atccctggcg gtgccgctgc gcgtccgttt atcacccacc ataacgcgct ggatctcgac
     7981 atgtacctgc gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag
     8041 cgtgtattcg aaatcaaccg taacttccgt aacgaaggta tctccgtacg tcataaccca
     8101 gagttcacca tgatggaact ctacatggct tacgcagatt acaaagatct gatcgagctg
     8161 accgaatcgc tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac
     8221 ggcgacgtga cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc
     8281 aagaaatatc gcccggaaac cgacatggcg gatctggata acttcgactc tgcgaaagcg
     8341 attgctgaat ctatcggtat ccacgttgag aagagctggg gtctgggccg tatcgttacc
     8401 gagatcttcg aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat
     8461 ccggcagaag tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc
     8521 tttgagttct tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg
     8581 gaagatcagg cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa
     8641 gcgatgttct acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca
     8701 ggtctgggga ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc
     8761 gacgttattc tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac
     8821 cccggcaaat gtcggggttt tttaattgtg ctgcgcgaaa gtagacaaca attttcttgc
     8881 ttcactattg gctgcctgca tcaccatcca cggactgaac gcccacggcg tggcatcaat
     8941 accgtgtaat acatcagcta aatcacacca ttgataatcc atcacttcat catcgttgat
     9001 ctgtaacgca ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc
     9061 actcggatcg gtggcgcggt agcgaaagtc aggatagata gattcaggag gcgtaatttc
     9121 cacgccaagc tcataacggc aacggcggat caccgcgtct tcgctgcttt ctcccagttg
     9181 tgggtgccca caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg
     9241 gcgggtaact aataattgcc ccttggcatt aaacagccaa ctggagaacg cgagatgtaa
     9301 gagggtgtct gccgtgtgtg cggcatactt ttccagcgta ccagtgggaa ctccctgtgc
     9361 attcaataaa atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat
     9421 cattatagat aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc
     9481 gttttgagcg gatgccgaca ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag
     9541 cgactttcaa tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc
     9601 aacagcagac tgaatatagc gcgcattaaa agattcatat tattctccat gctcattttt
     9661 cttcagcttg cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga
     9721 gacggattcc ttcaccaggt cagcatgatg ctgatagcca ttaaagaaga cgttcagcac
     9781 aacggcgcta agggttgcga gcataatgcc gctatgtagc agcggttgca gtacggccgg
     9841 taacttagaa aagaaatcgt gagagagcgt cggagtcatg ccaacgccga gactgatcgc
     9901 cacaatatag aggttgtaac ggttggtggt gtagttacag cgcgacagaa ttcgaatccc
     9961 tgtcgccagt accatgccga acatcaccag cccagcaccg cccagcacaa attgcggaat
    10021 ggaggcgacc agcaccgcca tttttggcac catgccaaac aggattaata taattcccga
    10081 agaaatacac acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgtga
    10141 aaatgacgtg tgggggaagc tgttaaacgt tccgcctatc attgtcccta cgccatcgac
    10201 acgcagcccg cgaataatat cgtgtgaaga gagtttacga ccgactattt cacccagtgc
    10261 caggaacatc cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat
    10321 ggaaacggga tcgaaaatcg gcataccaaa tgacatcggc gtgacaatcg caaaccatga
    10381 agcatcatgt agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa
    10441 tacaatcccc agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag
    10501 caagataaaa attaagacag caaaggagat acctaaataa acgggattac catattgcgg
    10561 attcccttta cctccggcgg cccagtcaat acccacctga atgatgctaa gcccgataga
    10621 agtaataacc acaccggtaa ccagtggcgg gaataaaggc atcaagcgac cgataagtgg
    10681 tgctaataat gtggtgataa aacccgcggc gatagtggca ccaaatatcc ccagcaggcc
    10741 gatatccggg ttcatcccaa tggctatcat tggtgttaca gcagcaaagg tcaccgacat
    10801 aatcaccggc aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac
    10861 gatcccgcag caaaagagat ccgagctaat gagcatcgca atagcttctt ttgagaggcc
    10921 cagtcggtca ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg
    10981 ttgcagaccg agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg
    11041 gcggtcttgc ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc
    11101 tttcgcccgc cagagtaaaa agcaaccgtt tacgttaata aaactgtcta aacacatgct
    11161 cgtggagtgc cagtttttgc gaggaagggg agttagagac aggaattgcg gaggcaatca
    11221 cataatcgtg gttaaaccac gaaaatgaga agagaacctg acaatgaaat tatctgtaat
    11281 tttctcatat tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt
    11341 tttatctgga tagcgctctt ttggtcggca atgttgaaat aatgaaagcg gcatagattg
    11401 aggggcaata atttgaactc agtcgcaaaa ttataattaa aacggtcatg ctacctaata
    11461 tatgcccgtt tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg
    11521 caaatgaata agtttatcgc tgctgaagct gcggaatgta taggctgtca tgcttgtgaa
    11581 attgcctgtg cggtggcaca caatcaagaa aactggccgc tgagtcacag tgactttcga
    11641 ccgcgtatcc acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc
    11701 aacaatgccc cttgcgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc
    11761 gtacaactgg acgagcaaaa atgtattggc tgtaaaagat gcgcaatcgc ttgccccttt
    11821 ggcgtcgttg agatggtcga tacgattgcg cagaaatgcg acctttgtaa ccagcgcagt
    11881 tccggcacgc aagcctgtat tgaagtctgc ccaacacagg cgttacgact gatggacgat
    11941 aaagggttac agtggataaa ggtggctcgc cagcgcaaaa cggcagcagg aaaagcgtca
    12001 tcagacgctc agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca
    12061 gataaaattt cagcgagtga acggaaaacc cactttggcg aaatatattg cgggctggat
    12121 ccactgcaag cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc
    12181 aactggcatt gcccgctgca taacgctatt ccggattaca tccgtctggt acaggaagga
    12241 aagattattg aagcggcaga actttgccac cagaccagtt ccttaccaga aatttgcggc
    12301 agggtatgtc cacaggactg tctttgtgaa ggcgcatgta ctttgaaaga tcactcgggc
    12361 gcagtctcta tcggtaatct ggaacgctac atcaccgata ccgcgctggc gatgggctgg
    12421 cgtcccgatg tcagcaaagt tgttccccgt agcgaaaaag tggcggtgat tggcgctggg
    12481 ccagcagggt tagggtgtgc tgatattctg gcgcgcgcgg gagttcaggt tgatgtcttt
    12541 gatcgccatc cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat
    12601 aaaacggtat taagccagcg gcgagagata ttcaccgcaa tgggaatcga ctttcatctt
    12661 aactgtgaaa ttggccgcga tattaccttt agcgatttaa cttctgaata tgatgcagtt
    12721 ttcatcggcg tggggactta cgggatgatg cgagcagatc tgccgcatga agatgcgccc
    12781 ggtgtcattc aggctctacc gttcctgact gcccataccc gccagctcat gggattgccg
    12841 gagtctgaag agtatccgct gacggacgtg gaaggtaagc gagtcgtggt attgggcggt
    12901 ggcgatacga caatggattg tttgcggact tccatccgcc tcaatgccgc cagcgtgacc
    12961 tgcgcgtatc gtcgtgatga agtcagtatg ccgggctcgc gcaaagaggt ggttaatgcg
    13021 cgcgaggaag gtgtcgagtt tcagttcaat gttcaaccgc aatatatcgc ttgtgacgaa
    13081 gatgggcgtt taactgcggt gggcctgatt cgtaccgcca tgggtgagcc ggggccggat
    13141 ggtcgccgtc gtcctcgtcc ggtagcgggt tcagagtttg aattgcccgc cgatgttctg
    13201 attatggcct ttggtttcca ggcgcatgcc atgccgtggt tgcagggcag cggaattaaa
    13261 ctcgataaat ggggcctgat tcaaaccggt gacgtcgggt atttacctac ccagacgcat
    13321 ctgaaaaaag tctttgctgg tggtgatgca gttcatggcg cggatctggt tgtcactgca
    13381 atggccgcag gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gcaggcatcg
    13441 tgatgaaatc gttaattatc gttaatccgg ctgactgtat tggctgccgt acttgtgaag
    13501 tagcctgtgt ggtcgctcac ccttcagaac aggagttgaa tgccgatgtc tttttacccc
    13561 ggctgaaggt acagcgactg gacagcatca gtgcgccggt gatgtgccat cagtgtgaaa
    13621 acgccccttg tgttggcgct tgccccgtgg gggcgctgac gatgggcgag caggtggtgc
    13681 agaccaattc tgcccgttgt attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa
    13741 tgatcaccat tcagtcgttg ccgggggata cccgacaaca aattgtgaaa tgcgatctct
    13801 gtgaacagcg ggaagagggg ccagcctgtg ttgaatcttg cccgacacag gcgctgcagt
    13861 tgcttactga aagagaactc aggcgagtcc gccagcagcg tattgttgcc agcggtgaga
    13921 atcctctgtg aaagaaatct ccgcgctgaa aggcgcggag aatctattag attgcccaac
    13981 cacccgcgta aaatgcgacc agtgcggcag taatgatcac cgtaccaata ttcaactttt
    14041 gccattcgcg agcaaagaca cggcctacga ccagtgtcac aaagcccagc ataataccgg
    14101 taacgatatt acaagtcaga acgatgaaca cggcacacac caggccagcc attgcgtcaa
    14161 taaaatcgtt gaaatccagc ttcgagacgt tacttaacat cagcaaaccc acgtacatca
    14221 gagcgggtgc agtggcgtaa ccagggatca aaaatgacag cggtgataag aacagaatca
    14281 gcaggaataa cgcccccact acggttgcgg tcaaccctgt tttacccccg gcggcggttc
    14341 ctgccgctga ttcgatataa accgctgcgg gcgctgcgcc caccagaccg gagaatattg
    14401 aacttactga gtcactggtc agggctttgc cgccattgat gatctggttg tctttatcca
    14461 acaaattcgc ctgaccggcg acggcacgga tggtgccagt ggcgtcgaac actgcggtca
    14521 tcaccaatgc cagtacactc ggaagtacgg ttggctggag tgcgcccata atatcgaggc
    14581 tgaaaatcag agacttacca tcttcgccag tcagacttgg catcgccacc agaccgtggt
    14641 atttcaccgc tgggtcaaag attaagccga taatcgaaat tgcgataatc accaacaaga
    14701 tcccgccggg tacgcggcac ttctccagac cgaagatgac cgccagcccc agcaagctca
    14761 tcatcaccgg gaaggaggta aacgcaccga gcgccactgg caagccttca atcgggtttt
    14821 tgataaccat acccacaccg ttagcagcaa tcagcagcag aaacaggccg ataccgatac
    14881 ctgtaccgtg agcgataccc atcggtaaat tacgtaagat ccaggtacgc acaccggtta
    14941 cggaaatagc ggtgaagatg acgcccatca gaaagaccgc gcccagtgcg acaggaacgc
    15001 taatttgttg cccgagtacc agactgaatg cggtaaatgc cgtcaaggaa atcgcacaac
    15061 caatcgccat tggcaaattg gcccacaacc ccatcagcaa cgagccgaag cccgcgacca
    15121 gacaggtggc aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa
    15181 cgacgataac ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac
    15241 gaacggtact gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg
    15301 gtgcgtccgg tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa
    15361 gttttattat tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga
    15421 acggtcatcg cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc
    15481 atagcgcagc tgctgtagcg gagtggcggt gggttccatc accacgaaat cagcctcttt
    15541 gccaggtaaa aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt
    15601 ggccaggtaa aacgcttcat atgccgagag gcgatagcct tgtaattgca atactttgta
    15661 ggcttcgttc agcgtttgca gcatgttgaa agtggttccg gcaccgatat ccgttcccat
    15721 gcccacttta actttcttct gccatgcttt tttcaagttg aataagccgc tgccgaggta
    15781 aaggttggag gtcggacaga aagcaatgct ggatttggtt tcgctgagac gatcccactc
    15841 tttttcttcg agatggacgc agtgagcaaa gacacagttt ttaccggtca ggccgtactg
    15901 atggtaaaca tccagataac catcatggtc aggataaagc gatttcaccc aggcaatttc
    15961 atctttgttt tcacagagat gggtatgtac ccacgtatcc ggatattctt ctttcaggcg
    16021 ttgcgccatc gccatctgtt caggagatga ggtcggggcg aagcgtggcg taatcgcata
    16081 tagcagacga ccatttttgt gccagcgttc aatcagttct ttgctttggt gatagctgct
    16141 ttcggcagtg tcgagcagat aatccggtgc gttgcggtcc atcatcacct taccggcaat
    16201 catacgcata ttgatatgac tggcggcttc aaacagcgca tcaacagatt gcggatgaac
    16261 agtgccaaac accagcgccg tggtggttcc gttacgtaaa agctgcttga tgaagaatgc
    16321 cgacatttcg cgggcgtact ctaaatcctc ataacgacgt tcagtaggga aggtgtgttt
    16381 attcaaccac tccagcaatt gttcaccata ggcccccacc atttcacttt gcggataatg
    16441 gatatgtgta tcgacaaagc ccggtactat cagtttgccg cgatagtcgc gcacgcgaat
    16501 agtgtcagga atttgatgct ttccgtcttc ccattcgcca aaccattcca cttttccctg
    16561 tttaatgagt aataaaccat cctcaataaa ccgcagcgca gaggcaatct cttccgggtt
    16621 atcgaccgta cgggtgacat caataaaact gcctcgtacc gctttcaacg tgtgttctcc
    16681 tgacatcatt gactccttta ctgttaatcc atctcttccg ctgaggtaat accaggcaga
    16741 acgttttcct gtcggtagcc accagggagg ataatattta ataaaatcgc agttaacccg
    16801 ccagcacaaa tagggttttc aactagtaca taaatagagg ctggcaatat tttaaaaatt
    16861 tcgggatcgt aggagacgcc aagtcctaaa cctaaagaag tggcgacaat aagtgtttct
    16921 cggcgcttta aaccgttggt gatgattatg cgaatcccag cgatggcaat catggaaaac
    16981 atcaacgtca ttgcgcctcc cagaactgcc gagggaatgg tcgtgaagaa gccgccaatc
    17041 atcggaaaca aaccgaggat aaccagcatt accgcgatgg ttcgcccgac ataacgtgaa
    17101 gcgacgccag tcatctgaat aaccccatta ttttgcgcaa acgtggttaa gggtaatgac
    17161 ccgacagcgg aggcgataac agaaaccaga ccatctgcca gcacgccgcc tttcagtcgg
    17221 gactgatact cttccccctg aatggggcgg cgggaaacca ttgccgtggc ggtgatatcg
    17281 ccgactgctt ccagcacgct aagcagataa atcgtgccaa ccaccaggaa ctgatggaag
    17341 ctaaaactaa agccgtattt gaacggatgc gggatggtga ttaacggcaa attgcgcata
    17401 ctgctgaaat ccaccatgcc caggcataac gatgcgatat agccgacaca tagcccaatg
    17461 gcgatccctc ccatgcgtag caacggactg cgacagcagt taaagccgat caccacaatt
    17521 aaaaccaata aaccaacgcc gagatgttcg taattgccga acgtaccgct gcttttggct
    17581 gcaaatcctc caccaaaatc gataatgccg actttaatca ggcttaagcc gatcatcagt
    17641 accacaatac cgctgacggt aggcgtaata acccgacgta aatagggcaa gataaatgaa
    17701 gatccgacaa ccagaaatgc gccaacgaag gagacgccga gaagcgacga catgattacc
    17761 tcttcgtgaa aaccgtcgct tttcatgctg ctgcccagcg caatcatgac cgtaacaaat
    17821 gaaaaattga ctgactgaat tgagagtagg ccagaaccga cgatgccgta gcggtttact
    17881 tgtaaccagg taccaatacc agaggcgatc atcgccatag aaacaagata ggcggttgtt
    17941 tcagcggaaa gctgtaaggc cgcacccacg attaacgcgg gggttaccat cggaacgaaa
    18001 attgccaaca gatgggtaat ggcaccaacg agagcctgat gaaagggagg gcgatcctcc
    18061 agttcaaata taaggtcaga acctgcatgg tttatatcag acatcccttc actcctttta
    18121 aagtaaattt gatcatctga attagtaaaa taaattccca cgaatttggg gcgcaggacg
    18181 ccttgagaac ggcttatctc attttcgcag tcactattta gtcatcgtcc aaacagagaa
    18241 acaggccgcc gaagcggcca tttattattt atattttttc cagcgcagtg agtattttct
    18301 ccggtgtgaa atgccattcg cgtaaccaga tgccgcatgc atcgtgaatt gcggtagcaa
    18361 tcgccggagc tgcgccattt acaccgattt ccgagatcga tttcgccccg aacgggccga
    18421 ctttatcgtc gctcggcacc agcacagcgc ggaaatcacg cggaatgtca ccaattttcg
    18481 gtgcgccgta actgcgtaaa tcacgcgtta acgggtgacc ttcggcgtca taaatgatct
    18541 cttcgctcat actgtggccg atagctcgca gggtagcacc gtagatttgt cccaatgcta
    18601 attccgggtt gaccggtgta ccgcagtcca gcaaggcgta gaatttatcc aggcggattt
    18661 cacccgtacg cgtgttgacg gcaacttcag cgaagtttgc gccatacggg aaggcgaaat
    18721 caggcgtgat ataactgcca gttcccacca gtgaaccaaa gccggtgccg gtttcgcctt
    18781 tatgagcaat atccccgaag ctgacttcgc ctttcttgcc gcgcacgacg cccggcgttg
    18841 ccagttgaac atctgccact gcctcaccca acatttgcgc gccgtggaac agaattttct
    18901 cccgcagatt ttccgctgcc aaacgcgccg cgttacctga gaagcaagta ccggacgagg
    18961 catatgcgcc tttatcaaac aacgcatgat cggtatcacc ggagataaca tgcacgtcct
    19021 gcggtgggca gtgcagcact tctgctgcca gtttcgtcac tacagtatcc agaccagtac
    19081 caatatccgc accgccagaa tgaacgataa aggtgccgtc cgattccagt ttgatcatgc
    19141 agttagcctg atcgatatcc gggatacccg atttctgcat gataatggcg acgccgcgac
    19201 cgatgtgcca gtcaccattt tgcggtttcg gtgacgacca ttggatcatc tcgcgaccct
    19261 gacgcaggat ctcttctagt gcacagctgg cggcggaagg aacggaggtc ggcgctttac
    19321 cttcaccgat tgcaccgaga attttcagct cttgcccttc gtgtacccgg ttacgttcga
    19381 taatttccag ttggtcgatc tgtaactgtt cagccagttc cgctaatgcc atggtgatag
    19441 cgaagttacc tttcggtgcg ccataaccct gataagcacc atttgggcaa atgttgctgt
    19501 agtaggtggt gacctggaaa tcgacgttat cgcacggata taacggcagc gacagcgccg
    19561 gtccgttaca cggtacggtg agtgagtggt tgccgtaagg gccagtgttg gcgcggaaat
    19621 ccatcttcac tgccgtcagg cgaccatctt ttttcgctcc cagtttgacg gtgactttcg
    19681 cgacgtgacg agaggtgtta gcaataaact cttcttcacg ggtgtagcgg aacagtaccg
    19741 gacgcccggt cacgcaggtt gcccaggcgc acacttcttc cagcaggatg tcctgtttgg
    19801 aaccaaaacc gccgccaact cgctctttaa tgacatgaac tttatgctgt ttcatgccca
    19861 cgaggcgcgc gacctggcgg cgtaagtgcc atggtacctg ggtggaggcg tggataacca
    19921 gacgatcgcc gtccatccgg gtaaagcaga tatgtgtttc agtcgggcac tgctgtgcct
    19981 gcgttgagtt ataggttcgc tcaatgatca catcggcatc ggcaaagcct ttgtccatat
    20041 cgccaatatg accatgaata ctggcggcga tatttttgcg agggcgagaa ccgatcggga
    20101 agttgatgat catatgctcg ccgcgctggg ctgcatggct gttatcgtct tccagagtat
    20161 ctggcgcacc agcaacatac accaccggtt catcgtgcac gacaggcgca tcttccgcca
    20221 ttgcttcgtc gatcgacatt accggcttaa gcacttcata ttcaacgtcg atgagcttca
    20281 atgcttcgag cgcaatttct tcactttctg cgacgaccgc agcaacacga tcgccgacgt
    20341 gacgcatttt cttgccgaac atacggcggt caagcggtga cggttccggt gcgctctgac
    20401 cacccggggt atagtagata tccgggcaat tcaggtgagt aataacgtga acgacgcccg
    20461 gtaaggcttc agctttgctg acatccagat gagtaatcag tgcgtgagcg tgtgggctac
    20521 gtaacatttt aatgacgcag gcgtcagccg ttacgcggtc ttcaacatag cagggtttcg
    20581 cctgcaccat tttcgcggca tcagttttag gataatgctt gccaatgact tcaaggtcgt
    20641 cacggaaagt cggagcgata tcaatggtgg cctgcggatt atttttacgt gcaaccgcca
    20701 gttcaatgac ctgatagtat tgttgccagc ctgcatcgcg gctgaacaaa ccagaaagcg
    20761 cgtcgtcgat ctcttcgcgg gtaggtgcgg cgatgcgatc gagaagatcg gtgataatca
    20821 gagctgcagc tggatcgtta taaccagact gcaccacgcc aacatcaacc attgcctgtt
    20881 gaaccagact taactcgttc catttgccca aagattctgc ggtacgaata tctgccttct
    20941 ctaactgtgc ggcaataagc aaggacgcgt taacgatatt accgttaaag attattgcgt
    21001 cagaaccggc aaacccgaag ccatcatcac tgttgcgtac agagtgcatt cccatgttaa
    21061 acaacagctt ttgcacgttt tcgcctggat taacggttag ctcctgaggc gcgccattta
    21121 aagtaaagtg gatgatcata cggcttcctc cccagcctgt tggcagtcgg catacaggtc
    21181 ggctaccaga actcccgtga tatagcgttt ataggccacg ctgccccgca aatcttcctg
    21241 cgggaaaatg gcgttggcga cagcctgttc aagtgcattg ccttccagat tttgtttttc
    21301 gacatcatgc agacgcagtg ctttactggc gacgccatcc agcgcaattc gcataccgtc
    21361 gtggtctgtc attgcaacgg ctgccgtcac gacggttaaa cctgcctgag aacggctaat
    21421 tttgcgggtc gcacaggtgc gatacggatc tttaatgata atttcggtta gcaggcgatc
    21481 gcatgggcag gccaggtagt cctcgattga cagcgtttcg ccgttgccaa aaaccagttc
    21541 agcatccagt gccaacagga cgggaagcag caccgactct tcctggcggg cggcgatttc
    21601 accaccaatg gtcgactgat tacgaacatg gcgtgagtaa acaaaaccga gggcttcacg
    21661 cagcgctgca ggaataaatc gcgcatcacg cagtggctgc aagcgagaca ttgcgccaat
    21721 ccgcagtgca ccgttatccc agtcaaccca gtccagttcc agatcctgta aggaaatggc
    21781 aatcttttta tcggtacggg ttggtgtagc attgagtttg ctgcccccgg cgaaccagac
    21841 ggcttcatcc tggtagcggc gcttcagttc cagcgcctgt tcgacagagt cgggcctgaa
    21901 aaattgttca atcatcttct ttccttattt aaccctcttc cctgcgggaa gagggcatct
    21961 gtcatttatg ccagcgcatc catccgacgc cacatactgg cagcggcttt tctggcttgt
    22021 gcataaatgg aatcgcaatc gaagttaaac tgacggtctt catagaccat cacaccattg
    22081 accatcacgc tgtgaacgct gcctgagccc ataccgaaag cgatatgccc ggcgatattg
    22141 tctgccagca gcggcgtcgg tgagttgtaa tcgcaaatgg ttaaatcagc tttgtaaccg
    22201 gcttccagaa gtccaaattt cgcgccaaag ttgcggctca tcagttcgtt accgttagtc
    22261 agggctttgg caaaactgtc aggccacagc ggaccacccg catcgcgatg tttaaagaag
    22321 gcaaatttca tctcttcaaa catgtccgaa ccaatgccgt ccgttcccaa cgccagattg
    22381 cggatgtcgc taaggtgatg gttgtagccg acatggttgt tcatgtttga acgggcgtta
    22441 tgcaccagga acgcatcgcg ctgattgagt agggcgatgt catctttcga caagtacagc
    22501 ccatgagcga ccagcgtttt gctatcgatg agatcgaatt gcgctagtcg tgccagcagg
    22561 tctttgccgt accagtggtg actgtaggaa acgtcgtaaa ggtcttccgc agcgtgaata
    22621 tgcaaaccac ggcctgtggc gttcacggct tcacgtagca tctccagacc ggcatccggc
    22681 acggtaaacg gcgcgtgagc accgatatgt gcttccacca gatacggctc ggttgctgct
    22741 ttcttcgcct catcaatctg acgggcgaag cggatgtttt cttctacacc ttcctgcaac
    22801 tctttgatgc cgttgttacg gtcagtagtt tcaaaacagg tcatcgcgcg caggccaact
    22861 tttaaaaatg cgtcgcgcaa tgtggagagc gacccgccga tatacgccgg agaggcatgg
    22921 tgatcgataa ccgatgtaca tccgctctta atcgcttcca gggaacaaat cagcccgctg
    22981 taatagagcg actcttcatc aagggcgcga tcgagccgcc accagagatt tttcagcgtt
    23041 gagatgaaat ccgggcaggg ggcgatgttt gccataattc cgcgggaaag ccccgagtaa
    23101 aaatggttgt gcgagcagac aattcccggc atcacaatcc gaccatgcat ctctttgtag
    23161 ctggcatcgg ggtagcgttg cgtcagggca tcgccgatag cgacaatcac atcattttcg
    23221 atagcgatat caacgccttc ctgcactttc gccgggtgta actgcactgc ggtgacattc
    23281 ttcagaatca acatgattac acctccacgc ggcccagcag ataatgatga tgctggtgga
    23341 catggctgat gatgcggcac atatcgttca gctccggtgg tacgttgttg aactgacctt
    23401 cgctgtcgat gtttaacacc cagctttggt tattcagtcg tactcgtacc cggcaatctt
    23461 ccacaaggaa gcctgggttg ctgctgttat caaagtcttg cgccaggctg aaaacggtga
    23521 ttttgtcttt gtatggttta ccgttccacg gacagaactg agcgcagttg ccgcattcgt
    23581 tacagtaagc gtcgaggtgc agcgtctgga atctattctg gaagcctggg accgcaatgg
    23641 agacgttggc gcggttcggg cagacatcca cacacttgct gcaaacgtag ttacattcga
    23701 ggcagcgagc ggcttcctgg gcgacaaacg cgtcacgatc gtcactgttc accagagtga
    23761 tagagatatc gcctttacgt tgatagattt ccgccggatt gacgttgttc cagtatttat
    23821 cgttctggtg ggaacggata ttttcccgac taaggatggc atcggtcgcc cgacgcgcgg
    23881 ttccgacagc agcgacaatg gaggatggtc cgcgctgtac gtcgccgatc ataaagacgt
    23941 cagtcagacg agtttcgcca ttgtggtcga cgtctggcca gccgtttttg tccagcggca
    24001 cgcccatcgc attcagggct tcagtatcct gctgttcacc aatggcggtg atcaggctgt
    24061 ctacatgcag tgttactgtt tcgttggttt caaccggacg gcgacggcct ttctcatcgg
    24121 gttcgccaag cgacataacg cgcaaggtta aggtgccatc agcatcgaaa cgttccggat
    24181 tattcaggaa acggaactcc acgccgtcgt gcaacgcttc ttcatactct tcgcgccatg
    24241 cgggcatctc ttgcagtgaa cgacggtaaa cgatcgttgc tttttctacg cctggaacgc
    24301 gtaacgccgc acgagcgcag tccattgcgg tgttacccgc cccgacaacg accacatgtt
    24361 tgcccagctt gagcgctgta cccttgttgt attcacgcag gaaggggagt gatttccaaa
    24421 cattttggtt gtcacccgcc agtttcacac cgctattttt atcagtgccg gtggcaatca
    24481 gaacatagtg gaagccctga ttttttaact gttcaacggt caaatcgggt gagcagccat
    24541 actcaaattt cacgccgtga tcggcaacaa aatcgatatc gtgctgaatt aactcagccg
    24601 gaattcggaa ctgaggaatg atatttttca ccacgccgcc cgcattggct tcgcgttcaa
    24661 acagcgtaac cggatagccc gctctggcaa ggaagtaacc tgctgccaga cccgccggac
    24721 ctgcaccaat cacggcaacc ggatggcgtg aaccagaacc ggctggtttg tgccagcgtt
    24781 gcttatattc atcccaacct ttttccagcg cgactttttt cagttcgcgg atattcagcg
    24841 cactgtcgta atccaggcgg gtacagttgt attggcactg gtgatcgcaa atatgaccgg
    24901 taatggcggg cagagcgttg cgttggtaga tgagttccag cgcgtcggca tagcggtgtt
    24961 cgccaagcag acggatgtat tccggaatat cttgcttgat agcgcaggca gtaacacagg
    25021 gggcaacgta gcagtcggtc agcggcaggt cttctgccac ttcaatacgc tcttctggct
    25081 tccagtgttt ctgggtgtat tccatggtta acgcatctgc tgccagtctg ttcagtcgtt
    25141 cgacgtcaac atggtcaagc ccccaggcgt cggagccttc cagctcgcgc atgcaggcac
    25201 ttaagcgcag atagccgcca ggtttcagca ggtcggttgc catagtaata gggcgaatac
    25261 cagtatcaaa aatatcgcgg atagtcagct gactggcacc accggaataa gaaatgggca
    25321 gtttgccgtc aaaggcgcga gagagaactg ctgcaacatt gatggagagc gggaacagcg
    25381 cacggcctga catatacatc tcttcaccag gcagtgcgcc tttattgttg atggtgccga
    25441 gagtgttagt cagttttacg ccaaagccga gtgatttttc ttttgccagt gccatcaggc
    25501 gttccagcat ttccagtgct tgcgtcagct tgaggtcgtg atcaaatgac tcttctttta
    25561 agcctatgta accgaaaccg cagacatcga ggatctcacg aacacgcgcg taccccagta
    25621 aggtcgggtt gagtttcaca aaggtgttga gccctttttc ttccagcatg tagcggcaaa
    25681 tggcttcgat ttcatgcgga ggacagccgt gcatggtgga gagggtgacg ccctgcacca
    25741 tactggtggg gatgcgagcg ggtaaggctt gcaagctttc gcgtttttcc tgcaatccgt
    25801 ggcgggctaa aaatgcgtca tcctggagta atttattcag cgtatcgcga tattgagcga
    25861 atttcggatg gtcagatgcg tccatcatat tgtcgatgaa ctgttgcatc ggcggttgct
    25921 taataccttc gaggttgtaa ccgacgctca tattaaagat gaacgattta ccggaatcag
    25981 aaggctggaa catcgcttcg agaaggtgca gggcaaacca ggctttgagg tattcatccc
    26041 aggctttaag cagggtaaat tcggtagacc attcggtgtt aaaacactcg tcttcggcat
    26101 cgatacaggg cttttccagc tccaggcggt caagaatttg gacggttttt agttcgatga
    26161 agcgtccgcc agtcagccag gaagtgacaa tattttgcgc gagctgcgtg tgcgggccag
    26221 cggcagggcc gacgggagtg gcacaggttt caccgaagac gctaacagtt ttacctttta
    26281 cgggtgagta aaactgttgc tcgggaatac caaagattga gcgttgttgt tggtattcat
    26341 caaatatgcg cgtcaaaagt tcctcaaacg gaatgggacg cataatatcc cccataaccc
    26401 tctccttagc ttatacagat atcattgagt gaccggtgtt gagaaatccg gtggtttagg
    26461 gggatagagc aacaatcgca ccactcttgc caggtttggc gggaaatata aaaaaatgtg
    26521 aggcggctcg aaacttaagg gctttttgtg aatgaactca caggtgccaa atgagaatta
    26581 aaatcactcc tgaaaattat tattgccagc ccatttatca atttttctct tttctcaaaa
    26641 tcagtgattc cttatcatta tgataatgag tgccggaagg attaaatatt tgaacgcaat
    26701 cgtttaacta cgttttctaa atttcagtat acctttcctt cgctgtaata aagtcgtccg
    26761 gagtatcaat atctaaaatt atttctgcat tttctatttc gacggaataa tgatctccca
    26821 taaggagtgc ctgacgcata ttggtgacat tgggtcgttg gattgcctgt atcaagcatg
    26881 gttttgacac taaaatcgga tggccgggga tgccattatg gagtggcagt attgcgccat
    26941 catttcgtaa cgaccagatt tttctaaaaa tatcgatggt gagggttggc atgtcaccgt
    27001 gggtgagaaa acaatgttct gtttgcactg cgggtactgc ggccttgacg gatgttagta
    27061 aaccctgcgc ataatctggg ttgtgaataa tagtgatatt gctctggttc gcataacgtt
    27121 cgtgcagttc attaccacga tagccggtga ctaaaataat tcggctacaa aactgcaacg
    27181 catttttgat acttgtatca agaattgttc cctgttccca gggtaacatc attttccatt
    27241 gccccattct tgatgataat ccggcagcgg taattataca gtcgatggct gacatagtgg
    27301 ttccattatt tataggggct aataacgcct gataattgcg ttacttcctg atggattaag
    27361 ttggggataa tttatcccag agaggtcata aagactcgtg aaaagtatag ttgacccatc
    27421 ggcattagtc attgatttag gtgcgcagaa acgtcccact gtgatttctg ttgttggtgc
    27481 tggggggaaa accagcttgc ttttttggct ggcagaactg ctccaggcta gcggcaggcg
    27541 tgtgttaatc acgacgacga cacatatgtt tatgccaacg tctcactggc ccgtggtttt
    27601 ctgtcgtgat cccgccatgc ttcctcatgc gtctcttaca tcccccattt cattttgttt
    27661 tcacagctgg aaagcgaacc agggaaaagt gcagggattt acgccagaag cgattgatgc
    27721 actggtgcaa cgaccagagt gtgacgtaat tctcattgaa gcagatggct cgcgtggaat
    27781 gccgttaaaa gcgcctgatg agcacgaacc ttgcataccc aaaagcagtt gctgcgtgat
    27841 tgctgtgatg ggaggacata ctttgggcgc gaaagtgagc acagaaaatg tccatcgctg
    27901 gtcgcagttt gctgatatta ccgggttaac acctgatgca accttgcaac tgagcgatct
    27961 cgttgcgctg gttcgccacc ctcagggggc gtttaaaaac gtaccgcaag gttgtcggcg
    28021 agtctggttc attaaccgtt tttctcaatg tgagaatgcg attgcacaaa gcgagctcct
    28081 tcaaccgctg caacaacacg acgtagaggc aatctggctg ggcgatatac aagaacatcc
    28141 tgcaatcgcg cgcagatttg tgaattagcg gcccgggtat tccgggttac tgaaaggata
    28201 tccattgagg ttggtatgaa tattttcaca gaggctgcaa aactcgaaga gcaaaattgt
    28261 ccgtttgcga tggcgcaaat tgttgatagc cgaggctcga ctccccgcca ttctgcacaa
    28321 atgttagtgc gcgccgatgg ttctatcgtc ggtacaattg gtggcggaat ggttgagcgg
    28381 aaggtgattg aagagtcact tcaggcattg caggaacgta agccgcgatt attccatgga
    28441 cgtatggctc gtaacggtgc ggatgctgtc gggtcagatt gtggaggtgc aatgtcagta
    28501 tttatcagcg tccatggtat gcgtccacgt ctggtgttga ttggcgcggg gcatgtcaac
    28561 cgggcgatag cccagagtgc ggcgctatta ggatttgata ttgccgttgc cgatatttat
    28621 cgcgaaagcc tcaatcctga actattccct ccatcaacca cgcttctcca tgctgagtcg
    28681 tttggtgcag cagtggaagc actggatatt cgccctgata attttgtcct gattgccacg
    28741 aataatcagg atcgtgaagc cctcgacaaa ctcattgaac agcccattgc atggttgggg
    28801 ttgctggcaa gtcgccgcaa ggttcagctt ttcctgcgtc aattgcgtga gaaaggcgtg
    28861 gctgaagaac atattgcccg tttacatgcg cccgttggtt acaacatagg tgcggaaacg
    28921 ccgcaggaga tcgccatcag cgtgctggca gaaatattac aggtgaaaaa taacgcgccg
    28981 ggtgggctga tgatgaaacc ttctcatcct tccggacacc agctggtggt gattcgcggt
    29041 gcgggagata tcgccagtgg tgtggcgcta cgtctgtatc atgcgggttt taaagtgatc
    29101 atgctggaag tggaaaaacc gacagtgatt cgttgtaccg tggcgtttgc ccaggccgtg
    29161 ttcgatggcg aaatgacggt cgaaggcgtt actgctcgcc tggcaaccag ctctgcggaa
    29221 gcgatgaaac ttaccgaacg cggattcatc cctgtgatgg cagatcccgc ctgttcattg
    29281 cttgatgaac tgaaaccgct ttgcgtggtg gacgctattc tggcgaaaca gaatttggga
    29341 acgcgggcag atatggcacc agtaacaatc gcgcttgggc cgggctttac tgcagggaag
    29401 gattgtcatg cggtaattga aacaaatcgc gggcactggc tcggtcaggt gatttactct
    29461 ggttgtgcgc aggagaatac cggtgttcct ggcaatatta tggggcatac cacccgacgg
    29521 gtgatccgtg ctcctgctgc aggcattatg cgatccaacg tgaaattagg cgatctggtg
    29581 aaagagggcg atgtgattgc ctggattggt gagcatgaaa ttaaagcacc gttgacgggg
    29641 atggtgcgtg gcttgttgaa cgacggcctg gcagtggtcg gtggttttaa aattggtgat
    29701 atcgatcctc gtggtgaaac ggctgatttc accagcgttt ctgataaagc ccgggcgatt
    29761 ggcggcggcg tacttgaggc gttaatgatg ttgatgcatc agggcgtgaa agcgacaaaa
    29821 gaagtgctgg aagtggctta aatgaaaatg gccggatggt attacccacc cggccttaaa
    29881 attactgaca aatcaccgtt ccggttttgc cttcaatccc ctctttcgct ttgctcaata
    29941 cggtaatcag tgcttcacga cctgcgcgag agcgagcaaa cgaggcggca gcttccactt
    30001 taggcagcat agaacctttc gcaaaatgac cttcttcgat aaagcgttca gcatcactta
    30061 atgacaggcg atcgagccac tgttcattct ctttaccgaa gttaatggcc actttttcta
    30121 ccgcagtcag aatgatcagc atatcggcat caatcatttc tgctaaacgg gcgctggccc
    30181 agtctttatc gataaccgcg ctggcaccac gcagatggtt accttcacga ataacaggaa
    30241 taccaccacc gccaacggtg atcaccacct ggcctgcatc taccagagct ttaaccgttt
    30301 ctttttcaat aatatcaacc ggttttggcg aggcaacaac acggcgatag ccgcgaccag
    30361 catcttcttt cagagtgtaa ccctgttttg tcagttgctc tgcttcttgc tcggtaaaga
    30421 acgaaccgat cggcttggtc gggttgagga atgccggatc gttagcgtcg acttcgacct
    30481 gagtaaccag cgttgctacc ggtttattaa tgccccgaga aagcagttct tctcttaagg
    30541 cgttttgtag atcgtaacca atataaccct ggcttaaggc aacgcagaca gacatcggca
    30601 gcattggcga gtgcgcttca gtttttgcag cagcttcaaa ggcctggtta atcatgccca
    30661 cctgcggccc gttaccatga gtgacgataa cttcatgtcc ttgggcaatt aaatcaacaa
    30721 ttgcctgaga agtaattttt accgctttca tttgtccggc caggtcatcg cccagcgcat
    30781 tcccgcccag ggcgagaaca attttcttac tcatttttcc tcactcatca ttcttgattg
    30841 tgtatagatc cctgacagtg gttagagcac gggagggaca aacggtttgc gtcgcaggaa
    30901 acgacctcgc ccggctttgc ctgtaaaggt gccgtcacag aaaatcgttt caccacggga
    30961 taaggttctg acaatcgcgc cctgacaggt aaaaccctcc catggcgagt agtcggcgtt
    31021 gtcgtggaga tggcgatgct gaatttgttg gctctgacgt gggtcgataa tcaccacgtc
    31081 gccatcggaa ccgggcgcta ataatccttt ttgcggccac aggccaaaca gcctggcggg
    31141 cattgcgctg gttaattcaa caaagcgttc cggtgttata cgtcccgtca tcacgccact
    31201 ggaaaacagt aactgcatgc ggttttccac accgggtaag ccatttgggc agcgactgaa
    31261 atcgccttta gaaatttgca ggcgttgagc catcgagaag gtgcagtgat cggtcgccac
    31321 cacgtcaatc gcaccatcgc tgatgccaca ccacagtttg tcctgctcgc gtacgttacg
    31381 cagcggtggg ctaagaatga acttcatgcc gtcttctgta tcgtaactgc gttcgtccaa
    31441 caggagatat tgtgggcagg tttcaaccca gactggctgg tgattcgcac gggcaagacg
    31501 cagataatcc agacctaagc cgttagacag gtgcacgata tagagcgggg cgttaccggc
    31561 aatttgtgcc aggttaatca tgcgggcgat ggcttccgct tcgcattcca gagggcgact
    31621 caaggcatga tagcgcggcg cggttaaccc tgcggcgata aattccgccc gcttgctggc
    31681 gatagccgca tcattttccg ggtgcacggt ggtcagcgcg ccggattcat gcagacggcg
    31741 taatgcctgc aaaacctcgt catcgttgag tttgtattga taggttaaat agagtttaaa
    31801 actgctcagt ccttcctcga ccatcatcgg gatttcgtcg aggattgcgt gattaatgtg
    31861 ctggatcaca ccgtgaaagc tgtaatcgat gaccgcttta tgggcggcat aaccacgata
    31921 aacctccagt tgatggcgta accgacagcc gtttgggcca aatcccatat ggtcaataat
    31981 ggttgttgta ccgccacacg cagctgcgcg ggtaccggta aaaaaatcat cacaactgcg
    32041 cgcgatgccg acatcaatat tgaaatgcgt atggacatcc acgccgccag ggaaaacgta
    32101 acagccagtg gcatcaattt cttcatacgg gagctgcggc gaaatattgt tgcccaactg
    32161 gcgaacaatc ccgctttcaa tcagcaaatc ctgtttggct tgtccatctg cgttaacgac
    32221 agtgccgttt ttgatcaata cgcgcatagc aaactccaaa aaccctccgg cgaaccggag
    32281 gggaataagt gaagagtaat tactctgttg ccagccagct taacgggatt gcagcgtaca
    32341 tcgcggcaca ggtcaccagg tgagatttcc aggttttttc gttaggtgca tgcgcttcag
    32401 gttctttacc cgggccaaag ccgatcaccg gaatgccgtg acggcccatg atagatacgc
    32461 cgttagttga gaaggtccac ttatcaacaa ccggcgcttt gccaaacagc ccttcgtagg
    32521 cattcaccag tgctttaacg gtgaagtgat cttcttccac tttccaggtc gggaagtagc
    32581 attcggttgg gtaaaccagg ccagtccagg acggacggtc gtagttgtac atagaaacaa
    32641 ccgcgttagc tttctgtact gcaggcaggg cgcggatttc gtccagcgcg ccttcccagg
    32701 tttcgcccca ggtcagacgg cggtcaatag agacggcgca gctatctgct acagcgcaac
    32761 ggcttgggga tgtgaagaag atttcagaaa cggtgagggt gcctttgccg aggaattcgt
    32821 cataacccag acgttgggag agttcttgta attcgccaag aatcggaccc attttgaaaa
    32881 tggcgttgtc accgcgttct ggcgcagaac cgtggcagct aacaccctga acatcaatac
    32941 gaatttccat acgaccgcgc tgaccacggt atacctggca gtcggttggt tcggtactga
    33001 ccacaaattc cgggcgaatg ccggattgtt caataatgta ctgccagcac agaccgtcgc
    33061 agtcttcttc ctgcacagta ccggtaacca gcagggtata ttcatcttcc agaccgaggt
    33121 ctttaatgat tttaccggca taaaccatag atgccatgcc gccttcctgg tcggaagtac
    33181 cgcgaccacc gatcagctca tcagtttcta tgccttcgta cggatcgaag tcccagtttt
    33241 tgatgttgcc aatgccgacg gtatcgatat gagcgtccat tgccaccaga cgcgggccgt
    33301 ggccgatata accgagaacg ttgcccatcg ggtcgatttc aactttatcg aagccgactt
    33361 tttccatctc ttctttaata cgatgtacta cgcgtttctc gtcgcagctt tcactgggaa
    33421 tagcaaccat gtcgcgcagg aagcgagtca tatccgcctg gtaatctttt gctttttcaa
    33481 gaatcagttt gaatggaata ttcttagcca ttatttttct ccagattttt tactccccgt
    33541 tttgcaggga gcagttccaa tagggtgatt aaggtgctac agcgtgtttg ccttcccaga
    33601 caacttcgcg gtagtgcttc acgtcggtgt cgccttcagt gctgataacc agcaccacgg
    33661 catctttgtt cagcgccagt ttttccatca ggctttgacg ttgcgggtga taatgaaccg
    33721 ctgcgagaac gcccaaaccg acagcgccgg attcaccgga gatgatgcgc gggtcgttgc
    33781 cgtacggatt acccagcacg cgcataccta atgcggcaac gctgtcctgg caggagatga
    33841 attgggtggc gcagttacgt aggatttccc agcccagcgg gttaggttcg ccacaggcca
    33901 ggcctgccat gatggtggcc atatcaccgc caacgttgac gatgtcgcct ttgacgccgg
    33961 agcgataaat acagtcagct ttgtcaggtt caacaataat gctgtgcaga ttttgcgggc
    34021 tatagacgtc gaccagataa cccagcacac caccggccat tgctccgaca ccggcttgca
    34081 gcagaacgtg cgtcggggtt acgcccattt cacgcatttg ctcgacggct tcatctgcca
    34141 gggttgcgta gccttgcatg atccaggttg ggattttggt gtaaccttcc catgccgtgt
    34201 cctgtaccac ttcccagccg tgctgctgcg cgtgttgcat ggtcaggcga acggtatcgt
    34261 catagttcat atctgtgacg atgcactcgg caccgaggtt cagaatggcg tcaacgcgtt
    34321 cctgagcaga acctttcggc atgtaaatca ccgcattctg tccgagttgc tgcgctgccc
    34381 acgccacacc gcgcccgtgg ttgccgtcgg tggtggtcgc gaaagtcatt ttttcgccga
    34441 tggcattttt caggtgctca aatgacagcg tttcgatatc aagatgatat ttttcacaca
    34501 ataattgagc gatagcgtac gcaccgccga gcattttgaa ggcgttcaga ccgaatcgtt
    34561 ttgattcgtc tttgacaaga attttcttca ccccaaaaag gtttgcgaga tcgtccagcg
    34621 cacaaagcgg tgttgggcga taaccagcta ttttctggtg gaactggcgc gccagtttgg
    34681 cctggctttg cgaaaagagc ggtgatgttt cgccgttgaa aaatttgtta tcggcgatat
    34741 caatcttcaa tgagaaaacg gacatatcct atccttttgg cctctggaaa tagagggccg
    34801 accagctgat cggcccgagg ggttatttca cgcgttcttg cgcttctttc agcagttgct
    34861 cgagcagtgc acctggtttg gcgtatttac gggacaggat catcgcggcg atgatataag
    34921 gcttccagct ggcttctttg taggtagcga tacggtattt ttcgaatacg ccttcagtca
    34981 cttcaccttc tttacaggat acgccgctga tatcagctgg caggcagtgc atgtacaggg
    35041 cttcgccatc acgggtcagt tccatcatct cttcagtaca atgccagtct ttgtgttgcg
    35101 cgttctgtgc cagacactgt ttttccagtg cttttaagcc ttcgtgatcg ttagcgcgca
    35161 gcaattcggt acgctgttcc atcactttgt aaggtgccca tgacttcgga taaacgatgt
    35221 ctgcatcttt gaacgcttct tccatgctgg tgacctgacg gaagctaccg ccagatgctt
    35281 tagcattgtt tttcgccact tcaaccacat ccgggatcag gtcgtagcct tccggatggg
    35341 ccagggtgac atccataccg aagcgagtca tcagaccgat gatgccttgt ggtacagaga
    35401 gcggtttgcc atagcttgga gagtaggccc aggtcatggc gattttttta cctttcaggt
    35461 tttccagtga accaaagtgt tcacgtaacc acgcgaggtc agccattgac tgagtcgggt
    35521 ggtcaatatc gcattgcagg ttcactaaag ccggacgctg tggcagtaca ccctgcttgt
    35581 aaccgtcatc aagtgcagcg ccaacttcac gcatataggc gttgcctgcg cccagataca
    35641 tatcgtcgcg aataccaata gcgtcggcgc agaaggagat catattggcg gtttcacgca
    35701 cggtttcgcc gtgagcgatt tgtgatttgc cttcgtcgag atcttgttgt gcaaggccga
    35761 gcaggttaag cgcggaagca taagagaagc gggtacgggt ggagttgtcg cggaatacgg
    35821 aaatacctaa tccactatta aagactttgg ttgagatgtt ttcagcacgc agtgctttta
    35881 atgctgcggc aacgtccagt acttgtttca gttcatctgg cgcctgttcc cacgttaaca
    35941 aaaaatcttt ctcgtgaagg tgagaggtca gcgaattgat atccttaatc agctcattaa
    36001 cagttttcat cataaagtcc cggtagtgga atgacaggca aagtggttta tttcactgtg
    36061 acttgccaaa gtgattggtg ttttcacagc tatacctggg actaacaata agtgtgccag
    36121 aattatccac ggggctgcaa aagactaatt tgacggataa tgtgtggggt tgatcacaaa
    36181 ttgatgccag cttccggttg tttgtaaaat acgaaaacgt ttggcttttt ttatcgcgtt
    36241 atttatttgc tgctaagtat cagaaataag ataaagggac aggctatctc tcttcagttt
    36301 gataaactca ccgccgtaat tctcatattg atatagaaat gtgatatctc ttccataatt
    36361 tcattagaaa atgataaaaa tcttatgttt ttatcaaagc caatttcgcc tgagttaata
    36421 tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag
    36481 gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc
    36541 agcgttttgc cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa
    36601 acttgtgtcg cgtcgccgga acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg
    36661 gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt
    36721 ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgt gaaaaagcat
    36781 ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg
    36841 ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct gattatgttc
    36901 gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca
    36961 tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta gttgttgatg
    37021 atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt gttgtacaaa
    37081 ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt aattttactc
    37141 atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc attggtcaat
    37201 tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg cataccagtt
    37261 tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc gagtgccgtg
    37321 ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg
    37381 tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat aagttgagcg
    37441 gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag cagcttctgg
    37501 aaagcgaact gttcggttat gttaaaggcg catttactgg cgcttctgcc aacggtaaaa
    37561 cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata ggtgatatgc
    37621 cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt ctgccgattg
    37681 gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaat cagaatttgg
    37741 cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc
    37801 cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg gtgcattact
    37861 ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct cccgatgtcg
    37921 tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg
    37981 aatatctggt taacgtagtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa
    38041 atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc
    38101 tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg
    38161 aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc atcggcattg
    38221 ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct cagtgaatcc
    38281 ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag aactgtattt
    38341 acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg acgaatttcc
    38401 gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg
    38461 ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg ctgaacatga
    38521 gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag
    38581 gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccaacaccgt acaggcacca
    38641 cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc
    38701 gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg
    38761 atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc
    38821 taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc
    38881 atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc agcgtttgca
    38941 ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg
    39001 cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg
    39061 cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc
    39121 tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt tcgtcatgct
    39181 caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc
    39241 gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc
    39301 tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc tgcggtccag
    39361 caatggacgt ggccgcagca cataacgccg ggagatgacg ttgagttata ggatcttcta
    39421 ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc gccagcgtaa
    39481 ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat
    39541 ggtggagctg aatcagtacg tcagtgccac cggcgagcag tttggcctgc gggttgtcag
    39601 ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca
    39661 tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg ttttggcgtc
    39721 agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg
    39781 gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca
    39841 ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa
    39901 tccagcagat tggggttacg gaccacgccg cttttagcat cgatgatcat ctcttcaaat
    39961 agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc cagcagtgga
    40021 ttaagaatat gccctgaatc atgaacgttg aggatgcggt tgatggtgac tttgcacagc
    40081 gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ccggtgggtt agtggtggtt
    40141 ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc
    40201 gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct
    40261 tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt
    40321 ttctctttta ataatagtgc cgcactgcgc agcgcaggcg cggcaacata gctctggcgt
    40381 gaggcaaatg cgccgggatc gaacggcgta acgtcggtat cttgagttga aataacgcga
    40441 acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac ggtgtcggca
    40501 ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaga
    40561 aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtgta gctaaaacag
    40621 gcgacgccaa cgccgcggcg caaattgcct tgctggttct ggcattctgc acggcgtttt
    40681 tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata
    40741 cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca
    40801 acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg
    40861 acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc
    40921 tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt cccccccgca
    40981 gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta
    41041 taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatggt aaaagcgtgg
    41101 cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg
    41161 ccaagcttgc tggtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca
    41221 aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat atccagcgcc
    41281 tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc
    41341 gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg ttgaataacg
    41401 ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg ttggacatta
    41461 cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct
    41521 tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc aatgctgacc
    41581 aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc aacggcgtca
    41641 ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg
    41701 tcaagtgtcc aggcatgccc agcagtagca aatggaatat caggcacatc ttcccaggta
    41761 aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact tacggcataa
    41821 ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat aacataatcg
    41881 tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca
    41941 cccgtagcgg ttgcttcccg cgcttccata gaaatcccct cgatttgaac atttggttct
    42001 tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttag
    42061 gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg
    42121 aataggggca gtgatacgta tcaatatgag tggcgataaa atcaatgtga taaattggga
    42181 ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata cctctacagc
    42241 gaatattttg ctgatgatac ttccctttgc caaatgaaat taacctcttt ctctattacc
    42301 acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga taaggcgtct
    42361 tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa tctctgggta
    42421 tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc aaatcatccg
    42481 atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg ctatatcgta
    42541 tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc atttcccccc
    42601 cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt agcagtagta
    42661 cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg
    42721 taccgaaatc ttcctggccg ccagtagggc aacgttgttg gttatggcca acgacaggga
    42781 aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat atctcagctc
    42841 cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc
    42901 tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt acggcttacg
    42961 cccataatga cacgatgctg gtaaataatg ggcaaagcgt gaaggctggg caaaaaatcg
    43021 ccactatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt cgttaccgtg
    43081 caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca aaatgctgat
    43141 ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa ggtttataat
    43201 gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc ccaagctcta
    43261 tacgagggtt cgattccctt cgcccgctcc aaacaaacat ctaccaacgt ctactaaagt
    43321 ctaccaatcc cccgaatatc aaggcctcca gcgattttcc tcgtctacaa ccgtctacag
    43381 tcatctacct aaatctacac acagaagtgt atagtaatgc gtataggaca aaatcgtttt
    43441 ttgggggcta tacacatgtt gctttcagac cttgctatcc gtcgcgcgaa gccaaaagag
    43501 aaagcctata cactcaatga cggcaacggg ctttcgttac tcattgagcc gaacggttcg
    43561 aagggctggc gattgcgata caggtttgca ggcaagccca aaatgttgtc gctgggtgtt
    43621 tatcctgagg tgtccctttc tgatgctagg acggcaaggg atgaagccaa aaggcttatc
    43681 gctggtggag ttaatccgag tgaggtcaga aaggcccaaa agcgagaaca ggcgaacaaa
    43741 tgcggcaaca cctttgaggc gatcgcccgt gagtggtatg aaaaacgcct ggaccgatgg
    43801 tctccctcat atgcagagga aatgatagag acgtttgaaa aagacgtttt tccgtacatt
    43861 ggaggtaggc ccatcgcaga gattaagccg atggaattgc tcgtggttct atcccgtctt
    43921 aatgaccgtg gcgcaactga aaaattgcgt aaggttcgcc agcgatgtgg cgaagtgttc
    43981 cggtacgcaa taattaccgg acgcgctgaa tataaccccg ctcctgatct ggcaagcgca
    44041 tttgcaccac acaaaaaaga gcattacgcg tatctgaaag ttgatgagct accggaattt
    44101 ttccgcgttt ttaatacgta caccggaagc cagatcgtca agctgggtat gcgattgttg
    44161 attcttacag gacttcgccc aggtgaatta cggcaggcag agtggcagga gattgatttt
    44221 gataatcgtc tgtgggaagt gccgaaagaa cgaatgaaaa tgcgccgtcc tcattgcgtg
    44281 ccactttcag aacaggcgat cgctattctg gaacagttaa aacacatgac ggggcagtat
    44341 ccgtttattt tccctggtcg aatacagcac agtaagccga tgagtgaaat ggctatgaac
    44401 gtgcttattc gtcgtattgg ctatgcaggg cgcgttactg gtcatgggtt ccggcatacc
    44461 atgagtacga ttttgcatga gcagggttat aactccgcgt ggatagaaac gcagctggcg
    44521 cacgtcgata aaaactcgat ccgtggcaca tacaatcacg cgcaatacct ggacggacgg
    44581 cgggagatgc tccagtggta cgccgactat atggactcac tggaacaggg cggtaacgtg
    44641 gtgcacggca agtttggtgt atgtgcataa aaacgactgg acaagtatac agatgtatac
    44701 taacgtacac ggaaagctac aggtaatatc cttacagatg gtttttcacg cagttacccc
    44761 atcatgtatg gacggcgctc cttgtaacaa cgaagatgga gtcttctacg cagctgaaaa
    44821 gctggatgct ctttaacagc ctttggtgct ttcctgatgc aggaaagaat agggatacat
    44881 gcacctttac aatgcatgta atgattcacc aatgaggctg cggagcgctg acaaagcgca
    44941 attgctctct tgcctgccca ccatgccctg aaaaagggcg ctctttaaaa ctactggacg
    45001 tcacgcctaa tcgtgtgacc ccgccgaaaa gcgggaatag ctctaccttg ctggagcata
    45061 ccaaccacca accaaacaat ctgaatttat cttatgcaat accacaacat gttttcatgt
    45121 tgtggttttt gtcgtgttcg taatttgacg caaataaaac tcttgctaag ccacaggagg
    45181 ggattttcgc gtcaacatta aggagattga taaatggcta ataacaattc attcatccgt
    45241 ctttctgagg ttatgcgccg tactggttac ggtcgcgcat ggatttatcg cctgatcagc
    45301 ctggggcgtt tccctaaacc ggtaaaaatt ggttcccgct ccgtggcttt tattgaaagt
    45361 gaagtcgacg aatggattaa ccagcgtatt gatgcttctc gcaacaacgc cgcgtaataa
    45421 tcttttggga gggggatatt ctcccccagc actaaacctg attcgatagc aggagttttc
    45481 aatgaaacgt ccgaatgtgc ccgtacaggg gcagggcttc gttcagcctg aaatcagcca
    45541 gaacgatata ttgattcgcc acaaagcaga gtcgcgtatt gattcccgcc tgtttgctga
    45601 acgcatcggc ataaagcaca aaagccttta ttcgcttatt cagaagcaca aaaccggatt
    45661 gcgtgaactg ggtatccttc cgtttcaaac ggagcgatta aaaaccaatc aacacggaga
    45721 gcgtgaacat aagtttgctc tgctgaatag cgatcagttt gattacatgc gccatatcgt
    45781 gagccatttc gccgctgagc gtatgaaaca cgttaagcaa tacttagagg aggttttatc
    45841 aaattatcgc gctggtggct ctgtacggcg cgaatatttg catgatgcag gaatggcagg
    45901 caaaacaaag gcgaccacga aagggtcgcc agtggagata aaggaaatca agcgcatcac
    45961 caactatgcc atgagtgtag ctggtggaca aggctatcag ccaggcggtc ggtttttagt
    46021 ggcttttaac cgccgtgggt atttctcgac aaaatcttca agggcaaatc gtttttgtgg
    46081 gtgttttttc actccgggaa gaggcgttaa ctcttcgggt tctgatatct ctttagcgcg
    46141 tttcagttcc agttggcgct caagtatgcg gtgcaaaagc tgtcttcgtt gttcggcaaa
    46201 aatatctgta atggattggt actttttgtt catgctgctg ccctgtaaag caatgcgccg
    46261 tagtacctca caccacggcg ctgatagtga ttattctgat tctttggcct tgcggcgctg
    46321 gcggcgtttg atctcgcctc gcatggctgt aacgataaat tgcgcagtgc tctcgcctgt
    46381 ttcttttact tgctccatag cctcaacaac ttcatgcggg gcgcgagcct gtaatttctg
    46441 tgatttgtta ttgatatggt ctctttccat ttctgcttcc ctttgtgatt actggaatcc
    46501 agtatacacg aaaaagacca aaacaaaacg cttgaagtgg attccactta gtgattatac
    46561 tggaatccag ttgagtgttt atcactcaat gcaaacgagg ctgaaaagga ttgccgtcct
    46621 ttcgcagcct ctgaccacca acgatagcaa gagtatcgag gcagctatga gaaatcatat
    46681 cacacacccg caagggcggg actcgcacaa cctgaataaa ttcacctggc gctttatgca
    46741 gtgcctgaca gacagcattg caatattcac cgttaccgct gccactgagc acgaagcacg
    46801 cgcccagctg ccgcacgcac atcttatttt tgtcgcccgt atccgtcagg aggtgactca
    46861 tgcttaaaac cttccgcgtc ttttcccgtg ctgttaacaa gcgtggctat accgttggca
    46921 tcacgcagaa cgtgaaggcc gaaaacgtcc aggctgcaat cagcacagta attaacaaat
    46981 cagctaaagc cggtttatcc ggcgtaatta tccacgccgt tcatgaaatt aagggggtgc
    47041 agtaatgcag gaaatgacac tggcagaagc ctcactgaaa gcgcaacaga tcgaagtaat
    47101 cagccgcatg attgaacgca tgattgaacg caaccacgaa gaaatagacg accatgacct
    47161 ttctgttatt gcgggactta tcaaacaact ttccagcaat gtggcggtgt ggttaatgac
    47221 ggaggaagaa aaacgcggcg aacaataacg gctaaaaata aacgggaaat aaatatcaag
    47281 acgactcctt caggaggcta attacatgaa atcgaaaata tctggcttaa ctgccagtag
    47341 caaaactcac cccgaattta tgtgcgggga tatttatcgc gataagtacg gcggcatcgt
    47401 gacgattatc agcacgagag aacgacgcat cacctaccgc cgtgaagggt atgactatga
    47461 ctgcgtgatg cccctgtatc agttcagtcg tgatttttcc ctgatgcagg ccgtgccacg
    47521 tggtaagcca gcaatacaaa agcagaggcg aacatccgaa agatgaaaaa catgattaac
    47581 gtattcagga gcaaaaaatg aaaaaagcac cgaatttaaa gctccagccg aaagacaaaa
    47641 tgaccgaagt cattatcttt gcaggtagtg atgcatgggc acatgcgaaa gagtggagcg
    47701 aatgggccgg aaagcatatt gcagcagatg atacaccgcc cgttattctt ggaccgaaac
    47761 aactggcgag cctggaagac acaaaaatca tcgacaaagg gcgcaattac gtgcgcgttt
    47821 atcgtgctgg tgaaatatca gagacagcac ttttacagat agcaacactg cttgcggtgg
    47881 cgggtgtaaa ggaggcacgt tgctacagcg gctttgttga ccagcagccg gaggactgga
    47941 caccgcgcat ggctggactg aaggatgatg cggaacgcgg caacagcctg gtgatcaatc
    48001 ttccggcaaa gacaaatttc accggaaatt atgatgacga attaaaaccc cgcgttgaat
    48061 gccgtcctga tggcgtttat tgggttacgc caaaggttga taagcaatca ggcgaaatta
    48121 tacggcctga aacgtggtta tgtgcttcgg ttgaattact gggcgctggt gtcattggta
    48181 acgagcatta ccgcgtgatg cgctggacag attcgacaac aaatcggctt gtcactatgg
    48241 ccgtgccttg ctgcggaata ggcgacagtg acggctggcg gttactgaaa gccaacgggc
    48301 ttaaagtcac gacaaacgga aaacacagga tccccctggc tgactggatg cagttaggcg
    48361 gacagcatga ggaatggcac ttaagcacga aagctggctg gcactttggc gcgtacatca
    48421 tgccggacgg tacggttatc ggcgaatctg acaagcccgt tttgtttacc ggaaaaagtg
    48481 ctgccattaa cggttattcc gtggcgggta cggcggatag ctggcgcgac agcgtggcgc
    48541 ggctggctgg tggtaatccg ttcatgatgc tgggcatagc gacgtcatta tccgcgccat
    48601 tagtcgggct ggtcggtgct gatggctttg gtgtgcattt tttcgaaaac tccacagcag
    48661 gtaagaccac aacacaaagc gtggcatcga gtttatgggg tgatcctgaa gcgcagcgat
    48721 taacctggta cggaacagcg ttaggcattg ctaacgaggc tgaagcacac aaccacgggc
    48781 ttttaccact ggatgagata ggacaggcag caagcgcccg tgatgtgtac gtatcggctt
    48841 atacgctgtt taacggcttc gggaaattac agggggcaaa ggacggcggc aacagagagc
    48901 tgaaaaactg gcgggccgtt gctatcagca ccggagaggt ggacattgaa acattcctca
    48961 aaaccgaggg gataaaggta aaagtagggc agcttgtccg cctgcttaat gtaccgatgg
    49021 agaaagccac gaaatttcac gaatacagca acggtaagga acatgcggac gcgttaaaag
    49081 aagcctggaa ggaaaaccac ggggcggcgg gtcgtgagtg ggttaaatgg ctgtcaggcc
    49141 accagcagga ggcaaaggat acggtaaggg aatgccgcga acggtggcgc aacctgatac
    49201 cggagagcta cggcgagcag gtccaccgcg tgggtgaacg tttcgccata ctggaggccg
    49261 cgcttgtgct ttccggtcat gtaactggct gggccgtgca ggaatgccgc gacgcgatac
    49321 tgcacaactt taatgcctgg gtgaaggtgt tcggtacggg gaacaaggag cacaagcaga
    49381 ttattgaaca ggctgaggcg tttcttgcgg cctatgggat gagcaggttt gcccctgtca
    49441 attatgaccc cgccagtctg ccgattcctg aactgtacgg ctacagggaa agcgacgggc
    49501 gttatgacga acctgttttg ttctacgtgc tgccggatcc gttcggctca catgtggcga
    49561 atggatttaa caaggatgcc gcggcaaaag ttcttcatga agccggaatg ctgaaaagac
    49621 catccagcgg tcgtgggtgg caaatcagga caccacggct aaaacacctg aaaggggcgc
    49681 gcctgagagt gtacggcctt ttgctggcgc aggaccacga cacggaaagc gattaatctc
    49741 gcgtataggc gatgaaaaaa tgttgtccca gttgtcccgc tacgtattaa ttgattgatt
    49801 ttactaaaaa tacaataaaa aagtgggaca acattacagc attttgggac gacatcgggg
    49861 tattttggga cgacatagcg acattttggg acgacacaac gagcgttaat taccctggct
    49921 ggtgactggg acaacataaa acccctcatg ttgtcccaaa atcggctaat gttgtcccag
    49981 ttttcggagt gtatttttaa ttaaatcaat ggattatgtg taagtgggac aactgggaca
    50041 actgggacga cgtgtttttg tatatatata aaaaaataaa ttgagaggat gaattcatga
    50101 gtgaaattac tgttaaaaat atctcacctg cggtagctgg ctggtgggcg aagttcagag
    50161 acgacgacgg cacggaatgg tacagcccga ttgctgcatg ggcgctttgt gaggttgatt
    50221 atttcggtgc ggggaacacc cgccgcgaaa tcttacccgt gctgaccagt gagcttggta
    50281 tgggtccgca cccatccgat gaaggctatt gcgcgtgtct gtatctgcct gatgaaaagt
    50341 tcacgctctc aggtgagcag gggtcttttg cctggtatcc ggtgaacagc aacagtaata
    50401 acggcaattc gggggctgaa tcatgacggc gaagcacaca aaaaaccata attccgtggt
    50461 gaagttcgta tctgcccgtg gcaggccaac gcttttagtg ccaggaaaac atctgcatta
    50521 ctgcgatgaa aatcatatcc cgatcgtgat tgtctggaag cgaacggtat acgccgatgt
    50581 gacatggctg tatgactcgc tggcgttgat gcaccgtgac ctgtttgagc gtgaagagtt
    50641 ccggctgaat attgaggaac gggcagaaaa aatctatgag caatatgcag caaacagcaa
    50701 aagagccgca cgggcaatca ctcaccattt catgacgctg tatgacctga aagcagaaga
    50761 tgcagaaaaa gcggcatgtg atttattcga tatgacaatg ggtattatcc aggagtaccg
    50821 gaacagggag cgcagaccat gacagcacag attgcagctt acgggcggct ggtggacgac
    50881 ccgcaggaaa aacagaccag caagggcaca ccgatgacgc tggcgcgtat ggcggtatct
    50941 ttgccatgca gtcaggcaca ggacggacag gcgacgttat ggctatcagt cctggcattt
    51001 ggtaagcagg ccgacgcgct ggcgagacat gaaaaaggcg accttatcag cgtggcgggt
    51061 aacatgcaga tcagtcagtg gaccggacag aacggagaaa tacggcaggg ctggcaggtt
    51121 atcgctgaca gcgtaatcag tgcccgttcg gcgcgtcctg gcgggaagcg ggggcagcag
    51181 gggaaggcga ccgaagcact ggacagagca aaacgacaga ccggacagca gggaaatcag
    51241 ccaccagcgg gaggtgatga cccttacggt gatgatattc cgttctgagc gacaggagca
    51301 caacatgaac cagcagacca cacaacagga ccagacagta cgtaaaccat cacggcgcat
    51361 accgtggcag cacatgacaa agcggcagcg aaaaaaacca cagacggatt aaccgcctgg
    51421 ctgaatactg gcccgagatt ttcaacgtgg agaatatgaa gcccctgaaa actggcgtga
    51481 tccgtgatgt gcttaaggac gctaaggaac gggaattgcc gattggtcac ggcgtattgc
    51541 gcggcgcgtt aatatcgtat atccacacaa ccagatacct gaatgctgtt atcgctggtg
    51601 gtgctcggta tgacctgaac gggcagcctt gcggcgaagt cacggaagaa gataaatcag
    51661 tcgccagtga actactgaaa cggagactgg cgcaaattaa gcagcagaac agccagcagc
    51721 aaacaaccgg agacgatggt gatgatagct gacagcgtgg cacagaaact tgaggagcgc
    51781 ggtttatggc gacgtgcggc gacccgatgg actgaggttc ttcttcatgc agaaacggac
    51841 agggaacgcg aggaagctgc cagacgtcgg gcgatatgta tcattaaatc ccgtcgtatg
    51901 cctgagcagt tcgtcacctt tggcgatgtg aaaaaggccg ctgacagaac gctggcagag
    51961 atggggatta atccacagga tgaatggcga aattacagct tcagtgatgc aggcgacgat
    52021 ttagcgctac cgtgatacgc atctcattgc cgcatttgct caattatcgc gattcgctat
    52081 aatcaccacc gaaaagccgt tgatggtgtt ctcctcgggc aactggagcc cgactgaatg
    52141 aagccattca acggtttttc ggcactcatt ccggtgtatc tgatcccaca catcgggatg
    52201 agtgaacgac gaaaacatca cggtgatttc gtcatgccgc gataagaatg ttcccctttg
    52261 ggcaactgga gccctctgtg cagaaggtta tcgcggtcag gttgtgataa aaacgcgcct
    52321 ctttgagcaa ttgaagctca ctgtaaagcg gcttatcaca gcaggctgta gcgggtgctc
    52381 atctctgggc aactgaagcc ctctgtacag aggcccgtta cggctgaccc tgaatgattt
    52441 cgccccgtgc aattgaagca cccagcggag ttattcaggg ttttttattg cccgtccacc
    52501 agcaccacat ctgtagcatt gatgcgtata cattcgccgt caattgcacc attccggccc
    52561 tcaccgatcc gcagcgagag catatagcca gacggcggga aatatgcctt gcgaacttca
    52621 ggatgctgta aataagactg ttgacgatat gggaatcaga cgggatcggc gacattacgg
    52681 gttatggaaa taacagccag gtaaattggt gaatatacgg caggctgcaa tcagagccga
    52741 acatagcggc ctccgtcacc attatcagac agtagcggta gtccttccat cacagcaaaa
    52801 tgctacccac ttcacagccc cttggaagcc tcttcgatgg ggtgaaaaat ccctgaaaaa
    52861 atgcgcaaaa aactgcgcaa aaaagtgcgc aaatcgatca taaaaccgat cacaaaatcg
    52921 atcacacaga caaacagagc aaagccagat atgacgcggc ttgcaggggg aaaggccacc
    52981 ggaaaaagtg cgcaaaaaaa tgcgcaaaac gaaacgcaac agccgcgcaa aacgaaaccg
    53041 atacgcgatg atcgaaaaag cgcgcaaatt taccggattc gggtaagcaa ttttgcctga
    53101 tttgggcaaa atacggtgac gccgtaacag tgacattgac accaaaacag tgacacacag
    53161 gagggccgat gaccgaacgt tcaccaacag agataattca cgggattatt gaagagcagg
    53221 aggaacgcga gttaatcctc atgattgaag agcagagaga acgccagcgc aaacaggagg
    53281 ccacgcagcc ggacagcgtg agcgctgaaa atcaggctga tgtgacgtga tggtgtcgaa
    53341 ctgagtagct ttaagcgact ctgtgactgg ttttaaacga gtcgcaaaaa gagccggaga
    53401 aaaaccacga atctggggtt ttttctaagc acgctgtaca cggtggaaca tatggtggtc
    53461 aggagacggc aggagcaacg ccagaacgcg cccccgtacc agtggcataa gccggagcgg
    53521 ttacggcggt aacttgttga ttaaaaggcg gctcaattct gagccgggta gctgacggcg
    53581 ttaactggct gatattgtta caaagctcat ttttgagctt tggcctaacc tactgatatt
    53641 ttcggaaacc tcaatttgag gagaccgcaa acgcaaaatt gcgctggcta cattcctcac
    53701 ttctgaggac tgttaacaag ctggctgaaa gacagtcaca agctacgaaa ttttcgtagt
    53761 ttaactttca tccagtgcat ccctgaatta cgggtatgta accacagcta ttacgaggtg
    53821 attgttgatg cgtgatattc agcaggttct ggaacgctgg ggagcgtggg cggcaaataa
    53881 tcgcgagaat gtgacctggt catccgttgc cgcaggattt aaggggatta ttccggcaaa
    53941 gataacatca cgctcacagt gctgtgacga tgatgcgatg gtgatatgcg ggtgcatagc
    54001 ccgtctttac cggaacaatc gcgatctgca tgacttgctg gttgattatt acgtgttggg
    54061 ggagacgttc atggcgctgg cacggaaaca tgggtgctct gacacctgta taggtaaacg
    54121 ccttcacaaa gcggaggggg ttgttgaagg catgttgatg atgctggatg tgaagctgga
    54181 gatggatcgg tatgttgagc gcgattcagt gacggcataa cgcgcataaa aaaaccgcag
    54241 tcatgaggaa actgcggtgg agaaatacgg cttgccaatc aatcatgaaa aacgtaatcg
    54301 cctgataatt atgccacgag acaaaggacg gtgcaccctg aatggtcata atgaccactc
    54361 agaccatcga ggcaaccata acgaacatcg ttacagttgg agcttttacc ccgttggggg
    54421 aaaaggtgca tacctgaatc atcattatgg tgatcaaggg catgtaaagc ccaccagcct
    54481 gattaaaggt taaccggaaa aagccaggta tggagggagt gattagccac gaacaagccg
    54541 gacaccacca gcagaaggta ttatgatttt cgtaatacct ttagcaaagc gtgaatcatc
    54601 attaccgtag ggatggtcat aatgaccaca ccttccccca ccggaacaag gaaggctggc
    54661 ttacgaaaat ttcgtaaccc tccccagcac aatgtattaa ataatcacta caggccattg
    54721 attaaactta attatcgcga atcgcgagaa ttagccccgc cggaacaagg aaagggaaat
    54781 tacgaaaatt tcgtaaaatc cccccacatc tcccacggtt gcccgttaag tgcaagcgca
    54841 caggcacgac ctcattaacc agttaccagc gctcacgctc aaacatccac agcaatgggg
    54901 ataaatcgca ctctggcgca atgttcacta tcggggcgag cattcaccgg aaaacgtcat
    54961 acaggcttac aggcggctta aaattcgatg ggcggaggac cggatgggaa tcgggagacc
    55021 tggacaaaaa gtgcaggcct ccatgacacg gtcaggacag tttgtgttta accagaatat
    55081 ttagcgcctg tattggggct tcgtggagct cccgcagaca ttcagggtca gtaacatgtt
    55141 cctggtatct tgttttctca agtaacaagc ggtgcatatg cagcatacgg gcaagagccg
    55201 cgcacacctc ctcaaatgcg gtatcccgct taccgtcact gtcagagcca caaaacagcg
    55261 gtacaacctc cacgccgtcg ctgtgggcct ttagccaggc atcaaccacg gttaaatcat
    55321 cggtgaagaa ccacgcactg ttagcgctga atcgcatttt ccaggcttcc ggtactggtt
    55381 gcggggagta tgaatatgct ggtggtatct tgtcgtgagt atccataggc tatcgttccg
    55441 tcattctgtt attcaccaca gcgctaaaaa cgccatgaag gagcagaata ccgattgcct
    55501 cgtttgctgt atgaggtgct taccacacat tgggtacaca gatgaatcag gcggaagtgt
    55561 gattaccgga aaaaatccat ttcagtttga gggaaaaaaa accgtagtgc gataacacta
    55621 cggcatgcca ccaaagaaac acaacaagag gtcaacaatg agtaatggac atgaccagac
    55681 gattttatca tgccggaaca aaagttatgt gacacagtcg gataaaaaca ggtaaagccc
    55741 accagcttca taggggataa ccggaaaaat tccggtaaat atttgcctga gtaagtcatc
    55801 aagcgcctgc taaatgtgta tagaaatgtg tataggattg attttagaac atgtggaaat
    55861 ggctttatta ctggtggcat ggcttgttgt tctgttccct tcgccttctt aaagtctact
    55921 aacttactga atcctaaggg atacggctta acgccttccg atagttttac tcactgtaac
    55981 caacactgtg actaactttg ttgaagtagt gagcagacct aaggtctcag tacatgctca
    56041 agtcaagaac ctacctttat cagcgtaatg gtgtctttta catccgtctt aggatgaaga
    56101 ccactagtcg cctgaccgct tcgcttcctt ctcacaaccg ttataaacta gcgtcagtat
    56161 ctttacggac caaggataga cgtaccgcta tggctcactc acggcacatc aagtcagcac
    56221 ttaaagcaat ccatgcagac aaccctaacg cctcttatga ggagctacgg gagcacctga
    56281 agaccattgt cgagtgggaa cttagtgtca gccgtgatga cctgaacgac ccggagtcct
    56341 accagcttta cgttgaccag tacgatgaca ttaagtccaa ccttcgggaa gctgtagcga
    56401 ccgagcgtct gactgtagac cagcatcgct atattaatga cgttatcggt gtgcttaagg
    56461 cctgtcagga tagactcaat ggcgatagct ctggcctatt gtcttacctt gagcctgaga
    56521 ctggcagtct aagaccgtcc gtctccctat ctgtattggc agaacctgaa gtccctgaac
    56581 ctaaagcact gaccctcgcg tctctcattg agcagtacga gcaggagaac gcccagaact
    56641 ggaagccagc gaccctaagc gaaaaccgag cgtcacactc tacgctaatc gagatattcg
    56701 actatctgga tattcaggat gtcggcaagg cgacccgtgc agatatgctc agagttcgtg
    56761 aagtcctcca gcagctaccg aagaaccgca agcaacgctt taagtctatg ccgctgtctg
    56821 acttgctgaa ccgggagtct aagactgact gtctggacgt cgttaccatt aacaataaat
    56881 atcttatcaa gatggctgct gtgtttaagt gggcggtacg caacgacttg atagctaaga
    56941 acctgaccga aggtcttgag ctgaaagtcc ctcagcgtaa agcctccgac gctcgtgatg
    57001 cgttcagtcc tgagcaggta gggcaactac tggtcgcagc taaagcgtac tcgcagaaga
    57061 cctcaggtaa gccataccat tattacgtca ccgctctggc tgcgattaca ggtgcgagac
    57121 ttaacgaggt ggcccaactt caggttaaag atgtcaggac cactgaggct gggaccgttt
    57181 ttatccatat caacgaagac gatagcagcc tgccgggtaa gagtatcaag aacgcccaca
    57241 gtgaccgctg tgtcccgttg gtagatggtg cttatggttt tgtattggct gactttatgt
    57301 cactcgtgga agaccgtaga aagactgagg gtgataatgc tatggtgttc aatggcctca
    57361 agctaatgaa gaacggctac ggtgagcagg ttagcaagtg gtttaaccga acgttgctgc
    57421 ccaaagtcct cgctgaccgt agcggcttag cttttcactc gtttagacat acagttgcga
    57481 cccagttaaa acaacatggg gtcgagttag cgtatgctca ggcgattatg ggtcatagct
    57541 cagggtctat aacttacgac cgttacgcga aagaggtcga ggtggaaacg ctgaaagaaa
    57601 aattagcgga gtcattgtca gtaaaaaaat agacggaaag taatcgcata gggatagcgg
    57661 aacgtgagga cgtaatggtc aaagaatttt tagttagcaa agaaaataag ccttcatatg
    57721 aatgtatgaa tactgctgaa acttgtttgg ggctggctgg aatggcttcg gaagattggc
    57781 aacgtgagca tcattgccgc acaggtataa tatttactgc ttttgctatt gaggcaatgt
    57841 tcatcttcta tagaaaacag gttgaccccg gctacgataa gacgcagaaa gagtgcagaa
    57901 agacaatgca caagaatacg ttaaagttgt gcggtataaa caattatatg gggacaaaac
    57961 cttaccaaat tatcaaagag tgtcttgagg taagagacgc tattgctcac ggcgattcgt
    58021 atacttcgag ctttaatttc tcggcggatc atttggataa ccaagacgat attgcaaaaa
    58081 gtgtcctggc ggtaaactct gaacaatttc ggggattaac tgttgagcgc cttgcaaaat
    58141 acattgaaat ggcaaagcat attgaccatg agatttctga taatggttac aggccttctg
    58201 aaggttacct tccagcagcc gagcgcagaa gtctgatgtt agcttttggc gtcagtggtg
    58261 tgtcaatgtg gcccagtgct tgagcgataa tacacctcac tatagaggaa tggaccctta
    58321 actatcacta taggggagag accctaagtc cccaccttaa tagcagtcac gacccataca
    58381 cacattcatt aagtctcagg cttccggtag cggaccgatt ggaaaccatc cgcagacgcc
    58441 tagtgttaaa tcaccttggc ctgagaccat tccttttaac gtaagctatc ttcccttttg
    58501 acaggcgggg agcagtagac cacatccgac tttaagttgg ctctgtgata atcgctgcgc
    58561 ggataactga agaaccaaga caccattact gaggtctgcc atccatttgt ggacaggcgg
    58621 aaactcaaag aggttagatc agtggtgtct gaggtcggag ctttgagtcc tgaccaggca
    58681 ggcttgagga ggccatgagt tcatcttgtt atggattgtg ttatcacaat tccaataaga
    58741 agaactttaa gtccagcctc cagtcctgtc agcccgaagt atactacggt cgctatgatg
    58801 tggttctcca tacttcgggt ctaactggag gtcctgaaca ctttcgagtc aagttctccg
    58861 aagttcatta ctgtttgact aaaatatcac tataggaatg acaccgagta aataactatt
    58921 tctgcctctg gccttgatgc gtcaagtcat gcggaaatat tcttctgtgt cttccacttt
    58981 aattacgtgg tcatccccga ccaatcaatc caattcgctt tcgtaagcac tttcggcggc
    59041 tccacttggt tgcgctgaat tggtgtggat acccgcaggg gacgagagtg cttaactaaa
    59101 ggagcagcct ttattatgtc tatcaaatta acgaccatgc aagccgctta ccttactcgt
    59161 gcaatgacta caggcggtgg attaattgag cttggagaat tggctaaggg attgggtaaa
    59221 cagaaagccc atgtggtccg taagctggaa gctaattttg acaaagagcg gctttccaaa
    59281 atggaaaccc acttaaccgt aatgggtggc aaccataaga atgtagccca gaccgtcaaa
    59341 acctatatgc tcgactacaa gactgctggc gctctggcga tgagctacga tcttcagtta
    59401 ggtgtagaag ttctcacgct tctagaggag gccgttcaga ctatcggcgc tatgaccggg
    59461 gccgtgcaag caggagatac acaagcagca cttgaggcgt cattattgtt ctctgagcgt
    59521 atgcgtaacc gtctggaata tacgtccggt gagacagaaa atgaaacccg tagcgtagct
    59581 ttgaagcgtc tgggacgtaa aggagctagc gtatgattga cccgcgtact cccgagggac
    59641 gtttgaccct acgttaccgt ggtctccgta cttcactact tttgtcgatg cttggtttgg
    59701 ataaagacgc cacggataac cgaccgtttt attctcgtaa tgagctaata gaaagattgg
    59761 ttatccgtga tatggaattt aaccgaggaa ataagtaagg aatcctctgg tcactgcgtc
    59821 agtaaatccc acaagtaatg cgatagccga agggatgata ttaattgctg cgacagaaag
    59881 tgattttatt attctctgtt ctttggatgc ctttattaaa accgcaaaga taaacactgg
    59941 aatgaatgca agtagggcta tggtgattat acgttcaagc atatacagtg tgtgccaagc
    60001 tatcccacct gttatatatc cagcgccgta tccaactgca taaaagcagg ccaagtaaag
    60061 caataagcta atagctaatt tgggcataaa tcattcctga gtgtcttggt gaaagatggg
    60121 cgtagcttag caaatagcca tatccctatt gatcgtttgt tccaatcata atgtcttcaa
    60181 ttgatcgttc agagtgatca aacctaaccc tatttaacct tttgagatga ccgatgcggg
    60241 gagatgccgc aggactaaac acgcctgtgg tcgaccgata gtctcaaccc tcaagaggag
    60301 taccaactta tgttattcaa caacaacgac tggaaactat cagtcaccga catcaatccc
    60361 tacgaaaata ctgtgagtct ggacggtcag tcctatccgc tgtctctggc gattaagacc
    60421 ctgattccgg gctacctgtc aggcttaccg tccaccagcc gcgaggcgat ggagttactg
    60481 gaagctctgg ctgaagctgg cgtgaccatt ggtaacttct tcagtaatga cctgatgacc
    60541 gcctatggtc gccgccagca gaacaagcga gtcgaagccg agcgtatcgc taaagagcag
    60601 cgcattcagg cggaccgtat gcgtgaagag aacatgaccg acgaagagtg gcagaaagag
    60661 ctacagcgtc gcgagcaggt caaagctgaa cgccggacct atggtgagaa cctccgcagc
    60721 gctactcatt ccgctggtcg ctcacgtgcc gctatcgtcg ctgaccttga gtcaggcggt
    60781 aactggatgg acagcttgta agcaagcgtc cctcatctga gaacacacgg tccctgactg
    60841 cctgttaggg acttcaagta tttgactcaa ggagattaat tataatgaat atgactaaaa
    60901 acgctttcca gtttggcatt gagcctgttc atatcaccga caacgacaac cttcaggtta
    60961 atgagggcct tccgactaac gccgaccctc aggtctatgc cctccagtta gctaagactg
    61021 ttaaggctat gctgaacggt gtacttaaag acgctcaaga taacatcccg ttccctgtag
    61081 aagtcctgcc gacccgtaac agcttaccga ctccaatcat tgctcacact ctggctgacc
    61141 gctctgtgct ggtccctgtc cgtggtggta agcgacctga agttgtgacc gctccgtctg
    61201 gcactgaaat caccgttgag ccgattgaac aggcgatcct cgtgtctcac caaacaaagc
    61261 tttgggacca gaagtcgacc actggtttca ctcaggggac gctccagcag gacgcactga
    61321 acatctgcga taacgtgatt cgtaccatta actctaagat ggttgacgtg ctggagtcct
    61381 caaagctgct gaagaccgta gaacttccgg ccctgactgg tagcctgacc gctaaggctg
    61441 acgctatcat ggataccctg tacgagaaca cggagtcttc tttcggttct gaggtttctg
    61501 actacggcat tatcgcccat gagtcacacc tcaaggctct ctcccgtctg gctgctaagc
    61561 agggcttcgg tggtgaagac gctatcgtcg atatgctggg gactgacgtc gcctattaca
    61621 acggtgaaga ccgtggcgtg ttcatgatgg ctaagcgttt tacggctctg agcttcggtt
    61681 gcttccgtca cgatggcgag agcattactg tggtcctttc acgtgatgga gactcccagt
    61741 ctcacgacct tgagattctc ggcaaggtgt ttgttgtagc tgaagctgct acgaccatca
    61801 agatgggtac tggctcggct actgctgtcc tgcctgtagt caaacgcctg agcttcacta
    61861 aagaagctaa ctaacaataa cgactattgg tcagacttcc gaactggatg tctggccttt
    61921 attttgtact agtaatctag ttcgtgaata cagaaagtag gagggccaaa tggacctaca
    61981 caaagtctat ctcgtggtct tcctgctaaa gctgggactg gtggtctggc tatgggctat
    62041 tagacgatga gttttgactg aaaaatgtga gacctcatct aatataaaga gcgagccaaa
    62101 agcccccata gcctgacctg agccagacca aaagggccgg gtagtcagac cgtgagttct
    62161 attcctttag agggaatatt acgtgcttca agtcttgacc ttctatctct aaatgtgtga
    62221 ctatcactac aaccaactgg attcacctgt ctttaagaaa caataggtta gctggttgtc
    62281 ttcgatttgt cgtaggctcc cttcgcccgc tccaatttat cttcacctga tcaatctgcc
    62341 atttttgctg tatctgattt tctatattat cttaattcaa tcgcttcaga cttttcgcaa
    62401 tcgccgtaat acttccccat tttgttgtaa ataaaaagcg tttacgcaat gcaatccttt
    62461 tgtaacaacc caggtaaaac accttatttt caagttgtta aataagtggt tatctacgct
    62521 tttaacaaac gattgatgga atattttatg aaagggaaat ctgcattaac tcttctgctc
    62581 gctggtatat tttcgtgcgg cacatgccag gcgacagggg cagaagtgac gagtgaatct
    62641 gtatttaaca ttcttaattc cacgggggca gcaacagata aaagttattt atcgttaaat
    62701 ccagataagt atcctaatta tcgtcttttg attcactcag ccaaattaca aaatgaaata
    62761 aagagccact ataccaaaga cgaaattcaa ggattgctaa cattaacaga aaacacacga
    62821 aagttaacgt tgacggagaa accctgggga acgtttattc ttgcttctac tttcgaagat
    62881 gacaaaacag cggcagaaac ccactatgat gctgtctggc tgagggatag tttatggggc
    62941 tatatggcat tagtatcaga tcagggaaat agtgtagccg caaaaaaggt tctgctcacc
    63001 ctgtgggatt atatgtctac gcccgaccag attaagcgga tgcaggacgt cattagcaac
    63061 cccaaacgac tggatggagt tcccgggcaa atgaatgttg tgcatatccg atttgacagc
    63121 aactcccccg tgatggcaga tgtgcaggaa gaaggcaagc cgcagctgtg gaaccataaa
    63181 caaaatgatg cgttaggtct ttatcttgat ctccttatac aagcaatcga cactgataca
    63241 attaacgctg aagattggca aaaaggtgac cgattaaaat cggttgcgct gctgattgct
    63301 tatctggata aagccaattt ctatgttatg gaagattccg gagcatggga agaagatgcc
    63361 cgattaaaca catcttccgt tgcactggtg acctctggac tggaacgttt gtcaaaccta
    63421 ctctcgaaga aagactctgt ttttgtctct gatttactac gcgaagctaa agtcaatgaa
    63481 ctggatgaaa ctctttcaac aacgaggttg aaccacctca tagataaagg ttatgaaaga
    63541 ataacgttac agcttgattt agggggagaa tccccaggtt atctggaaaa agataaacat
    63601 tacagagaag ccgatgcggc tttacttaat gttatttatc ctacaaatct ttcaaagata
    63661 aacaccagaa gaaaagagca ggttctaaaa atagttaaaa aactagcagg tccgtatggt
    63721 ataaaaagat acgagaagga taattatcag tcggcaaatt tttggttcaa tgatattaaa
    63781 accgataccg atcaaaattc gcatgcaaaa agagaaaaga gcttcattcc atcaacagaa
    63841 gcagaatggt tttttgactc atggtatgca aaatcagcag caattgttta taaagaatca
    63901 agaaaagaag agtatttgaa tgactcagta caatttatga atagatcatt ggcccaaata
    63961 acaggcgaga atatgattgg tgcaaatggc aggagcgtac cagaaatggc acttcccgaa
    64021 agctataact atatccacaa gtcgggaact tgcatgaagc gccaagccct atcattccgc
    64081 taaattggtc gaaagcttca atgactctca tgttgaaaga gatgtccaac cttatcaatg
    64141 atgagggaat taaatagttt tcataatgag ttacagcaca acgttgggta ttgactgagc
    64201 aagatgaacg acaaggacaa cgacatcagc cttatgagga gcaagaaaat gaaagcaaat
    64261 tcgaaaacga tcagaaggat gaaagttaat gttcggtctg aggaaagtaa atcgaaacac
    64321 tcatacgttt gaaaccactt ttcaaaattg gaaagaaaat ggcgaagatg ttgcattatt
    64381 aatgcccgaa tttagtgcta aatggctacc gatagcagaa gagagtggca gctggtctgg
    64441 atgggtattg cttggagaaa tatttccctt aatttcatct gagctcgctg ggatggcttt
    64501 aatgccagaa acggaaaggt taatagggga atggctcagt ttatccagtt ccccgttaaa
    64561 tctaaagcat cctgaactaa aatataatcg tttgcgcgta ggtaaagtat ttgatggagt
    64621 attgagccct gctcaaccac taataagaat atggacaggg gaattgaatc tttggttaga
    64681 taaagtcaca gt
//
LOCUS       DAZGXZ010000010        61824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000010 DAZGXZ010000000
VERSION     DAZGXZ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.010"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            306..1691
                     /gene="dcuC"
                     /locus_tag="ACHKJF_000757"
     CDS             306..1691
                     /gene="dcuC"
                     /locus_tag="ACHKJF_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0948170.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(1732..2412)
                     /gene="dpiA"
                     /locus_tag="ACHKJF_000758"
                     /gene_synonym="citB"
     CDS             complement(1732..2412)
                     /gene="dpiA"
                     /locus_tag="ACHKJF_000758"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP0948171.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2381..4039)
                     /gene="dpiB"
                     /locus_tag="ACHKJF_000759"
     CDS             complement(2381..4039)
                     /gene="dpiB"
                     /locus_tag="ACHKJF_000759"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP0948172.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4419..5477
                     /gene="citC"
                     /locus_tag="ACHKJF_000760"
     CDS             4419..5477
                     /gene="citC"
                     /locus_tag="ACHKJF_000760"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP0948173.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            5492..5788
                     /gene="citD"
                     /locus_tag="ACHKJF_000761"
     CDS             5492..5788
                     /gene="citD"
                     /locus_tag="ACHKJF_000761"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP0948174.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            5785..6693
                     /gene="citE"
                     /locus_tag="ACHKJF_000762"
     CDS             5785..6693
                     /gene="citE"
                     /locus_tag="ACHKJF_000762"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP0948175.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            6704..8236
                     /gene="citF"
                     /locus_tag="ACHKJF_000763"
     CDS             6704..8236
                     /gene="citF"
                     /locus_tag="ACHKJF_000763"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP0948176.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            8240..8791
                     /gene="citX"
                     /locus_tag="ACHKJF_000764"
     CDS             8240..8791
                     /gene="citX"
                     /locus_tag="ACHKJF_000764"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP0948177.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            8766..9136
                     /locus_tag="ACHKJF_000765"
                     /pseudo
     CDS             8766..9136
                     /locus_tag="ACHKJF_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046917 - triphosphoribosyl-dephospho-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase"
     gene            9187..10650
                     /gene="citT"
                     /locus_tag="ACHKJF_000766"
     CDS             9187..10650
                     /gene="citT"
                     /locus_tag="ACHKJF_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP0948178.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            10764..11570
                     /gene="rna"
                     /locus_tag="ACHKJF_000767"
     CDS             10764..11570
                     /gene="rna"
                     /locus_tag="ACHKJF_000767"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP0948179.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            11800..12210
                     /gene="rnk"
                     /locus_tag="ACHKJF_000768"
     CDS             11800..12210
                     /gene="rnk"
                     /locus_tag="ACHKJF_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP0948180.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            12348..12437
                     /gene="yldA"
                     /locus_tag="ACHKJF_000769"
     CDS             12348..12437
                     /gene="yldA"
                     /locus_tag="ACHKJF_000769"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP0948181.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(12441..13679)
                     /gene="ybdR"
                     /locus_tag="ACHKJF_000770"
     CDS             complement(12441..13679)
                     /gene="ybdR"
                     /locus_tag="ACHKJF_000770"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP0948182.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPSAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            13900..14328
                     /gene="uspG"
                     /locus_tag="ACHKJF_000771"
     CDS             13900..14328
                     /gene="uspG"
                     /locus_tag="ACHKJF_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP0948183.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(14449..16014)
                     /gene="ahpF"
                     /locus_tag="ACHKJF_000772"
     CDS             complement(14449..16014)
                     /gene="ahpF"
                     /locus_tag="ACHKJF_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP0948184.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(16185..16748)
                     /gene="ahpC"
                     /locus_tag="ACHKJF_000773"
     CDS             complement(16185..16748)
                     /gene="ahpC"
                     /locus_tag="ACHKJF_000773"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP0948185.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            17120..17866
                     /gene="dsbG"
                     /locus_tag="ACHKJF_000774"
     CDS             17120..17866
                     /gene="dsbG"
                     /locus_tag="ACHKJF_000774"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP0948186.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            18075..18977
                     /gene="ybdO"
                     /locus_tag="ACHKJF_000775"
     CDS             18075..18977
                     /gene="ybdO"
                     /locus_tag="ACHKJF_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0948187.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLRGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDSSVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKPEHLLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            19124..20344
                     /gene="ybdN"
                     /locus_tag="ACHKJF_000776"
     CDS             19124..20344
                     /gene="ybdN"
                     /locus_tag="ACHKJF_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0948188.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKSKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            20317..20946
                     /gene="ybdM"
                     /locus_tag="ACHKJF_000777"
     CDS             20317..20946
                     /gene="ybdM"
                     /locus_tag="ACHKJF_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP0948189.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(20947..22107)
                     /gene="ybdL"
                     /locus_tag="ACHKJF_000778"
     CDS             complement(20947..22107)
                     /gene="ybdL"
                     /locus_tag="ACHKJF_000778"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP0948190.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            22216..23304
                     /gene="hcxA"
                     /locus_tag="ACHKJF_000779"
     CDS             22216..23304
                     /gene="hcxA"
                     /locus_tag="ACHKJF_000779"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP0948191.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(23314..23511)
                     /gene="ybdD"
                     /locus_tag="ACHKJF_000780"
     CDS             complement(23314..23511)
                     /gene="ybdD"
                     /locus_tag="ACHKJF_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0948192.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(23693..25798)
                     /gene="cstA"
                     /locus_tag="ACHKJF_000781"
     CDS             complement(23693..25798)
                     /gene="cstA"
                     /locus_tag="ACHKJF_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP0948193.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(25979..26392)
                     /gene="entH"
                     /locus_tag="ACHKJF_000782"
     CDS             complement(25979..26392)
                     /gene="entH"
                     /locus_tag="ACHKJF_000782"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP0948194.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(26395..27141)
                     /gene="entA"
                     /locus_tag="ACHKJF_000783"
     CDS             complement(26395..27141)
                     /gene="entA"
                     /locus_tag="ACHKJF_000783"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP0948195.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDALVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(27141..27998)
                     /gene="entB"
                     /locus_tag="ACHKJF_000784"
     CDS             complement(27141..27998)
                     /gene="entB"
                     /locus_tag="ACHKJF_000784"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP0948196.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(28012..29622)
                     /gene="entE"
                     /locus_tag="ACHKJF_000785"
     CDS             complement(28012..29622)
                     /gene="entE"
                     /locus_tag="ACHKJF_000785"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP0948197.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(29632..30807)
                     /gene="entC"
                     /locus_tag="ACHKJF_000786"
     CDS             complement(29632..30807)
                     /gene="entC"
                     /locus_tag="ACHKJF_000786"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP0948198.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            31182..32138
                     /gene="fepB"
                     /locus_tag="ACHKJF_000787"
     CDS             31182..32138
                     /gene="fepB"
                     /locus_tag="ACHKJF_000787"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0948199.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(32142..33392)
                     /gene="entS"
                     /locus_tag="ACHKJF_000788"
     CDS             complement(32142..33392)
                     /gene="entS"
                     /locus_tag="ACHKJF_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP0948200.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAKIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDG"
     gene            33503..34507
                     /gene="fepD"
                     /locus_tag="ACHKJF_000789"
     CDS             33503..34507
                     /gene="fepD"
                     /locus_tag="ACHKJF_000789"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP0948201.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTYQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            34504..35496
                     /gene="fepG"
                     /locus_tag="ACHKJF_000790"
     CDS             34504..35496
                     /gene="fepG"
                     /locus_tag="ACHKJF_000790"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP0948202.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            35493..36308
                     /gene="fepC"
                     /locus_tag="ACHKJF_000791"
     CDS             35493..36308
                     /gene="fepC"
                     /locus_tag="ACHKJF_000791"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0948203.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(36305..37438)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKJF_000792"
     CDS             complement(36305..37438)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKJF_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP0948204.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(37654..41535)
                     /gene="entF"
                     /locus_tag="ACHKJF_000793"
     CDS             complement(37654..41535)
                     /gene="entF"
                     /locus_tag="ACHKJF_000793"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP0948205.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSEKIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSARRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQIATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(41532..41750)
                     /gene="ybdZ"
                     /locus_tag="ACHKJF_000794"
     CDS             complement(41532..41750)
                     /gene="ybdZ"
                     /locus_tag="ACHKJF_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP0948206.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(41753..42955)
                     /gene="fes"
                     /locus_tag="ACHKJF_000795"
     CDS             complement(41753..42955)
                     /gene="fes"
                     /locus_tag="ACHKJF_000795"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP0948207.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWKTQLNANWRGSYCFIPTERDDIF
                     SVPSPDRLELREGWRKLLPQAIADPLNLQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKKSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            43198..45422
                     /gene="fepA"
                     /locus_tag="ACHKJF_000796"
                     /pseudo
     CDS             43198..45422
                     /gene="fepA"
                     /locus_tag="ACHKJF_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
     gene            45462..45560
                     /locus_tag="ACHKJF_000797"
                     /pseudo
     CDS             45462..45560
                     /locus_tag="ACHKJF_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            45597..46217
                     /gene="entD"
                     /locus_tag="ACHKJF_000798"
     CDS             45597..46217
                     /gene="entD"
                     /locus_tag="ACHKJF_000798"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP0948208.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(46339..46491)
                     /gene="hokE"
                     /locus_tag="ACHKJF_000799"
     CDS             complement(46339..46491)
                     /gene="hokE"
                     /locus_tag="ACHKJF_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0948209.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(46842..46994)
                     /gene="hokE"
                     /locus_tag="ACHKJF_000800"
     CDS             complement(46842..46994)
                     /gene="hokE"
                     /locus_tag="ACHKJF_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0948210.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            46963..47232
                     /locus_tag="ACHKJF_000801"
     CDS             46963..47232
                     /locus_tag="ACHKJF_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948211.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            47436..48554
                     /gene="ybdK"
                     /locus_tag="ACHKJF_000802"
     CDS             47436..48554
                     /gene="ybdK"
                     /locus_tag="ACHKJF_000802"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP0948212.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFTSSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            48620..48868
                     /gene="ybdJ"
                     /locus_tag="ACHKJF_000803"
     CDS             48620..48868
                     /gene="ybdJ"
                     /locus_tag="ACHKJF_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP0948213.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            48933..49301
                     /gene="ybdF"
                     /locus_tag="ACHKJF_000804"
     CDS             48933..49301
                     /gene="ybdF"
                     /locus_tag="ACHKJF_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0948214.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            49395..50048
                     /gene="nfsB"
                     /locus_tag="ACHKJF_000805"
     CDS             49395..50048
                     /gene="nfsB"
                     /locus_tag="ACHKJF_000805"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP0948215.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            50156..51403
                     /gene="ybdG"
                     /locus_tag="ACHKJF_000806"
     CDS             50156..51403
                     /gene="ybdG"
                     /locus_tag="ACHKJF_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP0948216.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(51471..52847)
                     /gene="pheP"
                     /locus_tag="ACHKJF_000807"
     CDS             complement(51471..52847)
                     /gene="pheP"
                     /locus_tag="ACHKJF_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP0948217.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(52949..56092)
                     /gene="cusA"
                     /locus_tag="ACHKJF_000808"
     CDS             complement(52949..56092)
                     /gene="cusA"
                     /locus_tag="ACHKJF_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP0948218.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSTVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(56104..57327)
                     /gene="cusB"
                     /locus_tag="ACHKJF_000809"
     CDS             complement(56104..57327)
                     /gene="cusB"
                     /locus_tag="ACHKJF_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP0948219.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(57343..57675)
                     /gene="cusF"
                     /locus_tag="ACHKJF_000810"
     CDS             complement(57343..57675)
                     /gene="cusF"
                     /locus_tag="ACHKJF_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP0948220.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(57833..59206)
                     /gene="cusC"
                     /locus_tag="ACHKJF_000811"
     CDS             complement(57833..59206)
                     /gene="cusC"
                     /locus_tag="ACHKJF_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP0948221.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGG"
     gene            59363..60046
                     /gene="cusR"
                     /locus_tag="ACHKJF_000812"
     CDS             59363..60046
                     /gene="cusR"
                     /locus_tag="ACHKJF_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP0948222.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            60036..61484
                     /gene="cusS"
                     /locus_tag="ACHKJF_000813"
     CDS             60036..61484
                     /gene="cusS"
                     /locus_tag="ACHKJF_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP0948223.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        14895 a        16722 c        15491 g        14716 t
ORIGIN      
        1 ataatataaa tcgtcaacct atgctcatgt tagattattt tttgagcgca cagccgcagc
       61 taacaccatt gcaattaaca aatttgcatc aatccaccat caatttacac acattattat
      121 gtgataattg ccaaccgcta aatatgcgtt ttgttatcta tgtataaaaa cagcaacttc
      181 aatgtcttaa tggcagtttt tcttgatttt aatcagcatt catcgccaat ttattgggca
      241 tattttttcc ttaagcttta ggaatttttt attatttact ttggggcctg gagacaggaa
      301 aaattatgct gacattcatt gaactcctta ttggggttgt ggttattgtg ggtgtagctc
      361 gctacatcat taaagggtat tccgccactg gtgtgttatt tgtcggtggc ctgttattgc
      421 tgattatcag tgccattatg gggcacaaag tgttaccgtc cagccaggct tcaacaggct
      481 acagcgccac ggatatcgtt gaatacgtta aaatattact aatgagccgc ggcggcgacc
      541 tcggcatgat gattatgatg ctgtgtggat ttgccgctta catgacccat atcggcgcga
      601 atgatatggt ggtcaagctg gcgtcaaaac cattgcagta tattaactcc ccttacctgc
      661 tgatgattgc cgcctatttt gtcgcctgtc tgatgtctct ggccgtctct tccgcaaccg
      721 gtctgggtgt tttgctgatg gcaaccctat ttccggtgat ggtaaacgtt ggtatcagtc
      781 gtggcgcagc tgctgccatt tgtgcctccc cggcggcgat tattctcgca ccgacttcag
      841 gggatgtggt gctggcggcg caagcttccg aaatgtcgct gattgacttc gccttcaaaa
      901 cgacgctgcc tatctcaatt gctgcaatta tcggcatggc gatcgcccac ttcttctggc
      961 aacgttatct ggataaaaaa gagcacatct ctcatgaaat gttagatgtc agtgaaatca
     1021 ccaccactgc tcctgcgttt tatgccattt tgccgttcac gccgatcatc ggtgtactga
     1081 tttttgacgg taaatggggt ccgcaattac acatcatcac tattctggtg atttgtatgc
     1141 tgattgcctc cattctggag ttcctccgca gctttaatac ccagaaagtt ttctctggtc
     1201 tggaagtggc ttatcgcgga atggcagatg cgtttgctaa cgtggtgatg ctgctggttg
     1261 ccgctggggt attcgctcag gggcttagca ccatcggctt tattcaaagt ctgatttcta
     1321 tcgctacctc gtttggttcg gcgagtatca tcctgatgct ggtattggtg attctgacca
     1381 tgctggcggc agtcacgacc ggttcaggca atgcgccgtt ttatgcgttt gttgagatga
     1441 tcccgaaact ggcgcactct tccggcatta acccggcgta tttgactatc ccgatgctgc
     1501 aggcgtcaaa cctgggccgt accctttcgc ccgtttctgg cgtagtcgtt gcggttgccg
     1561 ggatggcgaa gatctcgccg tttgaagtcg taaaacgcac ctcggtaccg gtgcttgttg
     1621 gtttggtgat tgttatcgtt gctacagagc tgatggtgcc aggaacggca gcagcggtca
     1681 caggcaagta aatagtaatg ccggaggcaa gtttcctccg gctttatcat gtcacccact
     1741 gtggtatatg cgttgcggtc tgccaacttt gccgtgaaca atttcagcaa taatcagatg
     1801 gcggctggcg caatattcaa gataacgcct ggcagtggtg cgactgatgg tcagtgcctg
     1861 cgccaccgtt tctgccgtat gttgcacacc aggctcttta aacagttttc gcaccgcgtt
     1921 tagcgtcaag ggatcaatgc cggtcggcag ttcgtcttta ggttcaccgc gagcataggc
     1981 attaaacatc tcatcaattt gcttctggct ggcgctatca atactttcca gcatatgttt
     2041 acgctggcgg aaacgggtta gcgtttgccc cagccgttca taggcaatgg gcttaatgag
     2101 ataatcaaat acaccacaac gtacggcttc agacaccgtt tccatatcgc tggctgcagt
     2161 ggtaaacacc acgtcgccgg gataatgcgc ctgcaccagt tcatgcaata aattaatccc
     2221 tctgccgtca ggaagatagt tatcgagcaa gattagcccc ggcttaaaac gctcgatcat
     2281 cattcgggcc tgcgccaggt ttcccgccag taatatctga ctgaatccgg gaatgtgacg
     2341 aatatattcc gcatgcatct ctgccagcgg cgtttcgtcc tcaacgatca atagggttaa
     2401 tggagctgtc attaggtttc actttcggaa tatagattga aaacaaggta ccgcagggat
     2461 cattatcttc gagagtgata acaccaccgc agcgcgttac gtagctggca atcaagtaca
     2521 acccaatgcc atgttcaccg ggctcgtcag cacgcgtact gaccccctgc tcaaatattt
     2581 tgtctcgtag agactctgga acgccgcagc cctgatcggc aacttcaatc accacatcat
     2641 cgccttcatc gctgaggaat aattcaacga tcttgtttcc ttcatcgcta cgcaggctgg
     2701 cttcgaaggc gttatcaagt aaattgccca caatggctgc aaactcggtg ctatccagtc
     2761 ctggcggcag ttgcgaaagc tggctaccgg ggacaatgat catttttagc cccagttccc
     2821 gggcgcgctg cactttacca aaaagcagcc ccgccacctg gcgatcggca aacgcctcac
     2881 gcaggctgtc aataagctgt tgctgggcct gagactcccc ctgcaccatc gccagcacgc
     2941 gatcatactc tttcatctgc aacagaccat tgagcgtcga catccaattg agatgctcgt
     3001 gtcgcaatgt acgaaggctc tcgacgtatt gttttatttg cgtcagttgc gcattgaggg
     3061 tggaaatttc gtctttacta cgaaagctga taatggcccc cagcaaatca tcacctgaac
     3121 gaatagcttc ccgattggca ataacgctca gaccgttaaa gttagccacc acatcctgac
     3181 gtttttcatc aatctgttcg gtaaagaaat cggcgggcct gaccacttca gcaatgggtt
     3241 tacccaacca ttgccgtccg ggagagctca gccccagcat ctttcttgcg ttacgattga
     3301 tggcggtaat gtaaccatgc ggatccaccg caatcagccc ttcataaacc gaactaaaca
     3361 gcgcctcttg ctgacggacc acgcgtgcga tttgctttgg ctccatgccc atcatttgcc
     3421 gacggatatg cgctgccagg aaccacgaca gcaacatcag aatccctaac agcacgacaa
     3481 acacgcctgc catcggtaat aaaaactcag cccgccagct atcgatttta ctcaccaggt
     3541 agccaatcga caccacgccg atgacttttc catcgtcatc aaagattggc gttttggcgc
     3601 gcatcgccat ccccattgac cctttcccgg taatgaagta gctctcccct ttctccagcg
     3661 cgccctgttt ggtgaactgc ataggataac caattttctc cggattagga tggtaaaggc
     3721 ggatcgagtg ccggtcccca atcaccacat aatcaaaatc ggtatctctt tgtaatttgt
     3781 tagcgatggt cgccagccgt ttgtagtcac gcgttttcac cgcagagatg acactgtcat
     3841 tggaggcaat aattttcgcc tgattcattg ccatgtcgcg tacatgaagc gttaaatagt
     3901 cctcaaaact ggccgtaaaa tattgcgcca gggctgcaat gacaaatatt gagaacacca
     3961 gaatcagcaa aaagatacgc agcggaaatg ccagtctttg gaaaaatgca aactgtttat
     4021 tctcgttaag ctgcaacatt gttttccttg cgggttgtcc cgagcctctt tgacggatgt
     4081 aattaaaatg ttgaatcaaa atacatagat attaattatg atcgtggcaa attttattca
     4141 ttaaatcaat taaatcactt aaacctgctg gtcatttcgt aattacatta attaaaaaac
     4201 ttaaaaccat taaataaata aaaaaccact aactctatgt gaaataaatc aaaatttcac
     4261 gccgaaatac tccttaggat gtatagcgaa aggagaaaaa gatatacctc gatcaccccc
     4321 ctttctccca agtgaaaata aaaggttatc agtttgcaac attgaacaac attcgttgca
     4381 aatcgataac aacatgcacc ttcaggatac tatttattat gttcggcaat gatattttca
     4441 cccgcgtaaa acgttcagaa aataaaaaaa tggcggaaat cgcccaattc ctgcatgaaa
     4501 atgatttgag cgttgacacc acagtcgaag tatttattac cgtaacccgc gatgaaaagc
     4561 ttatcgcgtg cggtggaatt gccggaaata ttattaaatg cgttgctatc agtgaatccg
     4621 ttcgcggtga aggactggcg ctgacattag ccactgaact gataaacctc gcctatgagc
     4681 ggcacagcac gcatctgttt atttatacca aaaccgaata cgaggcgctg ttccgccagt
     4741 gcggtttttc cacgctgacc agcgtacccg gcgtgatggt gctgatggaa aacagcgcca
     4801 cgcggctgaa acgctatgcc gaatcgctga aaaaatttcg tcatccaggg aacaagattg
     4861 gctgcattgt gatgaacgcc aatcccttca cgaatggtca ccgttatctg attcaacaag
     4921 ctgcggcaca gtgcgactgg ttgcatctgt ttttagtcaa agaagattct tcacgcttcc
     4981 cctatgaaga ccggttggat ctggtgttaa aaggcaccgc cgatattcca cgcctgactg
     5041 tgcatcgcgg ctccgaatac atcatctccc gcgctacgtt cccttgctac ttcattaaag
     5101 aacagagcgt cattaaccat tgttacaccg aaattgatct gaagattttc cgtcagtacc
     5161 tcgctcccgc gctgggtgta actcaccgct ttgtcggtac tgaacccttt tgtcgcgtta
     5221 ccgcccagta caaccaggat atgcgctact ggctggaaac gccgactatc tccgcaccgc
     5281 ccatcgaact ggttgaaatt gagcggctgc gttaccagga gatgccgata tccgcttccc
     5341 gggtacgtca actgctggcg aaaaacgatc tcacggctat cgcgccgctg gtccctgcag
     5401 tcacgctgca ttatttgcag aacctgcttg agcactcccg ccaggacgcg gcagctcgtc
     5461 aaaagacccc cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg ccgttgcagg
     5521 cacccttgag tctggggatg tgatgatacg catcgcccca ctcgatacgc aggatatcga
     5581 cctgcaaatc aatagcagcg ttgagaaaca gtttggcgat gcaattcgca ccaccattct
     5641 ggacgttctc gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg acaaaggcgc
     5701 actggactgc attttacgtg cacgactgga agccctgctg gcacgcgcca gcggtatccc
     5761 ggctctgcca tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg taaaactcgc
     5821 acccgccgca gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag caactccttc
     5881 atctacccgg ctgatgccct gatgtttgac ctcgaagact ccgtggcatt acgtgaaaaa
     5941 gacaccgccc gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg cgatattgaa
     6001 accattgtgc gtgtcaacgc gctggattcc gaatggggtg ttaacgacct ggaagccgtc
     6061 gttcgcggtg gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca ggatgttctg
     6121 gatattgaaa aagagatcct gcgtatcgaa aaagcctgtg gtcgtgaacc cggcagcacc
     6181 ggcctgctgg cggcgattga atctccgctg ggcattaccc gcgcagtgga aatcgctcac
     6241 gcttccgagc gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg caacctgcgt
     6301 acagaacgct ccccggaagg aactgaactg ctgttcgcac gctgttccat tttgcaggcc
     6361 gcgcgctctg cgggtattca ggcgttcgat accgtctatt ccgacgctaa caacgaagcc
     6421 ggattcctgc aagaagccgc ccacatcaaa cagctgggct ttgacggcaa atcgctgatc
     6481 aacccgcgtc agattgatct gttgcacaac ctctacgcac caacccagaa agaagtggat
     6541 cacgcccgcc gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct cggcgtggtt
     6601 tccctgaacg gcaagatggt ggacggtccg gttatcgatc gcgcccgtct ggtgctctct
     6661 cgtgcagaac tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc agaaaattga
     6721 acaatctcaa cgacaagaac gggtagcggc ctggaatcgt cgcgctgaat gcgatcttgc
     6781 cgctttccag aactcgccaa agcaaaccta ccaggctgaa aaagcgcgcg atcgcaaact
     6841 gtgcgccaac ctggaagaag cgattcgtcg ctctggttta caggacggca tgacggtttc
     6901 cttccatcac gctttccgtg gcggtgacct gaccgtcaat atggtgatgg acgtcatcgc
     6961 gaagatgggc tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt gccatgcgcc
     7021 gttggtagaa cacattcgcc agggcgtggt tacccgcatt tatacctccg gcctgcgcgg
     7081 tccactggcg gaagaaatct cccgcggtct gctggcggaa ccggtgcaga tccactctca
     7141 cggcggtcgt gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg ctttcctcgg
     7201 cgtcccgtcc tgtgatgaat tcggtaatgc caacggctac accggtaaag cctgctgcgg
     7261 ctccctcggc tatgcaatgg ttgatgccga caacgcaaaa caggttgtga tgcttaccga
     7321 agaactgctg ccttatccgc ataatccggc aagcattgag caagatcagg ttgatttaat
     7381 cgtcaaagtt gatcgcgttg gcgatgctgc aaaaatcggc gctggtgcta cccgtatgac
     7441 cactaacccg cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg tcaactcagg
     7501 ctacttcaaa gaaggtttct ccatgcaaac cggcaccggc ggcgcatcgc tggcggtaac
     7561 ccgtttcctg gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg ccctcggtgg
     7621 tattaccgcg acgatggttg atctgcacga aaaaggtctg atccgcaaac tgctggatgt
     7681 acagagcttt gacagccatg ctgcgcaatc gctggcccgt aaccccaatc acatcgaaat
     7741 cagcgccaac cagtacgcta actggggttc gaaaggcgca tcggttgatc gtctcgacgt
     7801 ggtggtactg agcgcgctgg aaattgacac ccagttcaac gttaacgtgc tgaccggctc
     7861 tgacggcgta ctgcgtggtg cttccggtgg tcactgcgat accgcgattg cctctgcgct
     7921 ttccatcatc gtcgcgccgc tggtacgcgg tcgtattccg actctggtgg ataacgtgct
     7981 gacctgcatc accccgggct ccagtgtcga tattctggtc acagaccacg gtatcgcagt
     8041 taacccggca cgtccggaac tggcagaacg tctgcaggaa gcgggcatta aagtggtttc
     8101 cattgagtgg ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc cgattgaatt
     8161 caccgaccgc gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg atgttgtgca
     8221 tcaggtgaag gaataagcca tgcacctgct tcctgaactc gccagccacc atgcggtgtc
     8281 aattcccgag ctgctcgtca gccgggatga aaggcaagca cggcaacacg tctggctcaa
     8341 gcgccatcct gttccactgg tctcctttac cgtggttgcg cctgggccga ttaaagacag
     8401 cgaggtcaca cgccgaattt ttaatcatgg cgtgacagcc ttgcgtgcct tagccgcaaa
     8461 acagggctgg caaattcagg agcaggctgc actggtttcc gccagcgggc cggagggcat
     8521 gttgagcatt gccgccccgg ctcgcgacct caagctcgcc accattgagc ttgaacatag
     8581 tcatcctctc gggcggttat gggatatcga tgtcctgacg cccgaaggcg aaattctctc
     8641 ccgccgcgac tattcactgc cgcctcgccg ctgcctgttg tgcgagcaaa gcgcagccgt
     8701 ctgcgcgcgt ggaaaaaccc atcaactgac cgatttactc aaccgcatgg aggcactgct
     8761 gaacgatgtc gatgcctgca acgtcaacta aaaccacaaa gcttgcgacg tcattaatcg
     8821 atgagtacgc cctgctgggc tggcgcgcca tgctgactga agtcaatctg tcaccgaaac
     8881 caggcctcgt ggatcgcatt aactgcggtg cgcacaaaga tatggcgctg gaagatttcc
     8941 accgcagcgc gctggcgagg ggggcctgcg ctggctacag cgcgaggcgc aaacattatt
     9001 gcaaaaaggg ggcattcgaa cccccgccga tctcgattat ctccggcagt tcgacaggga
     9061 gtgtatcgaa cgaaatctca gtccaggcgg cagtgccgac ctgctgatcc ttacctggtt
     9121 tttagcacag atttaattat ttaagcactt gataaatttg aaaatattaa ttttcggaga
     9181 acccgtatgt ctttagcaaa agataatata tggaaactat tggccccact ggtggtgatg
     9241 ggtgtcatgt ttcttatccc tgtccccgac ggtatgccac cacaggcatg gcattacttc
     9301 gctgtgtttg tggcaatgat tgtcggcatg atcctcgagc caattccggc aacagcgatc
     9361 agttttattg cggttactat ttgcgttatt ggcagtaatt acctgctctt tgatgccaaa
     9421 gaattagctg acccagcgtt taatgcgcaa aaacaggcgc tgaaatgggg tctggctggt
     9481 ttttccagca ccacggtatg gctggtattt ggcgcattta tttttgcatt agggtatgaa
     9541 gtttccgggt taggtcgtcg cattgccctt ttcctggtga aattcatggg caaacgcacg
     9601 ctgacgttgg gttatgcgat tgtcattatc gacattctgc tggcaccgtt tacaccgtcc
     9661 aacaccgcgc gtaccggggg tacggttttt ccggtcatta aaaacctgcc gccgttgttt
     9721 aaatcattcc ccaacgatcc gtccgcgcgt cgtattggcg gctatttgat gtggatgatg
     9781 gtcattagta ccagtctgag ttcgtccatg tttgtcaccg gtgcggcacc aaacgtgctg
     9841 ggtctggagt tcgtcagcaa aattgccggt atccagatta gctggttgca gtggttcctc
     9901 tgcttcctgc cggttggggt tatcttgctt atcattgcgc cgtggctttc ctacgtgctg
     9961 tacaaaccgg aaatcacaca cagtgaagaa gtggcaacct gggcgggtga tgaactgaaa
    10021 accatgggtg cgctgacacg cagagagtgg acactgattg gccttgtatt gctcagctta
    10081 ggtttgtggg tttttggcag tgaagtcatt aatgctactg cagttggtct gctggcagtt
    10141 tcgctaatgc tggccctgca cgttgtgcct tggaaagata ttacccgcta taacagcgca
    10201 tggaacacgc tggtcaacct ggcaactctg gttgtgatgg ctaacggcct gactcgttct
    10261 ggctttattg actggttcgc cggtaccatg agtacgcacc tggaaggatt ctcaccaaac
    10321 gcaacggtga ttgtactggt tctggtgttc tactttgcac actacctgtt tgccagcctg
    10381 tctgcgcaca ccgcaaccat gctgccggtt attctggccg tcggtaaagg tattccgggc
    10441 gtaccaatgg aacaactgtg tatcctgctg gtgctgtcta tcggtatcat gggctgtctg
    10501 acgccgtatg caaccggtcc tggggtgatt atttacggct gtggctatgt gaaatcaaaa
    10561 gattactggc gtcttggcgc aatcttcggg gtgatttaca tctctatgtt gctgttggtt
    10621 ggctggccga ttctcgccat gtggaactaa tacgttcgcc aaacgctgaa taaatcatca
    10681 cgcccgccag ataagcccac ctggcgggct tttttatgat ttaatagata gtattcagtg
    10741 tatgtatgag ttccacaccc attatgaaag cattctggcg taacgccgcg ttgctcgcgg
    10801 tttctctgct tcccttctct tctgccaacg ccttagcgtt gcaggcaaaa cagtatggcg
    10861 attttgatcg ctatgtcctg gccctctcct ggcaaaccgg attttgccag agtcaacacg
    10921 atcgaaatcg taacgaacga gatgaatgtc gcctgcaaac cgaaacgacc aacaaagctg
    10981 attttctgac cgtacatggt ctgtggccag gattgcctaa atcggttgct gcccgtggtg
    11041 ttgatgaacg ccgctggatg cgcttcggtt gcgctactcg cccaatcccg aatctaccag
    11101 aagcgcacgc cagccggatg tgttcatcgc cggaaaccgg attatcactg gaaacggccg
    11161 ctaaactaag tgaagtcatg ccaggagctg gcggacgttc ctgcctggaa cgctacgaat
    11221 atgccaaaca cggtgcctgc tttggttttg atccggacgc atacttcggt acgatggtac
    11281 gcctgaatca agaaattaaa gagagcgaag ccggaaaatt ccttgcggat aattacggta
    11341 aaacagtgag ccgccgtgac tttgacgccg cctttgccaa aagctgggga aaagagaacg
    11401 tgaaagcagt taagctaacg tgccagggta accctgcgta tttgactgaa attcagatct
    11461 cgatcaaagc tgacgccatc aacgctccgc tttctgcaaa ctcattcttg ccacaaccac
    11521 acccaggcaa ctgtggcaaa acctttgtga ttgataaagt gggttattaa tcatctgatt
    11581 tacaaaacac gctaattcgt cttccataaa ccgtgacgat taagaattat acataatgcg
    11641 ctgcattgag gaattccctg aatgtgacgc aaatcacttc aaagagtgag caacagtcag
    11701 tatcatcatg tgagttaaac cctcgccgcc tgacggtgag ggttttcttt tgggtatgtt
    11761 tatctgcgac actcgcagta ccgacgacat ggagtaaaaa tgtccagacc aactatcatc
    11821 attaacgacc tggatgccga acgcatcgat attctgctgg agcaacccgc ctatgctggt
    11881 ttgccaatcg ccgacgcgtt aaacgcagag ttggatcgcg cccaaatgtg ttcgccagaa
    11941 gagatgccac acgacgtggt gacaatgaac agccgggtta aattccgcaa tcttagcgat
    12001 ggcgaagtgc gtgtgcgcac gctggtgtat ccggcaaaaa tgaccgatag caatactcag
    12061 ctttccgtta tggctccggt aggtgccgca ctgctggggc tgcgcgttgg cgattccatt
    12121 cactgggaac ttccgggcgg cgttgcaacc caccttgaag tgctggaact cgaataccag
    12181 ccagaagctg ctggcgacta cctgctttaa tcccatctgc acaagcgata actcgctttg
    12241 cccagaggat gcactcgcat cctctttacg caaaatcagc aatttttcct ggttctcact
    12301 gttcactttt gctgcaaaat tattccgttt cgtttgtggg aataaccatg gcagaagcat
    12361 tttacatcct gataggattt ctgattatgg cggcgattat cgtcatggct gtcctttacc
    12421 tcgaaaacca ctcctgacga tcatattgtt ccccccggca tcgcattcac cagacctgaa
    12481 accgccttct gcgccgcctc tgcgctttgt gcactgggta ccagaatcac cttacggcac
    12541 tcctcttcac gtttttcgaa aatctcatat ccccgggcgg cctcttcaaa cggcatatag
    12601 tgggtaacaa tttcttctgg tttcagtaat cctttctcaa ttaacggtaa taattctccc
    12661 agccatgcgt gaacgtgggt ctgtcccatt ttaaacgtca accctttatc aaaggcgtcg
    12721 ccaaacagga aaccgtgaat aaatccagcg tagacgcccg gtacgctaac aatgccgcca
    12781 cgcctgaccg ccgcaataca ctggcgcaac gctttaccgc tgctgccctc cagtttcagg
    12841 ttagtcagca ccgtttccgt ggtgctgcct ttcgcttcaa aaccgacggc gtctattact
    12901 gcatccacgc cccggtgacc tgccgtttgt tcaataattg actgtgccgg atcgctatct
    12961 tcatcgaaat taatcgggat cgcgccgtag cggtcagcgg cgaaacgcaa gcggtaggga
    13021 tgatgatcaa caacaaaaat ctgttccgca ccgagcaacc gtgcacaggc gattgtcaac
    13081 aatcccactg gaccggcacc atagactgca acgcttgaac cttgttggat ctgcgcattt
    13141 tttgctgcct gccatgccgt tggcagaata tcagaaagga aaagggcttt atcatctgaa
    13201 agcaaaggcg gtactttaaa cggccccaca ttccctttag ggacgcggac atattccgcc
    13261 tgtccaccag gaacgccacc atacaggtga ctataaccaa acaatgccgc gggagctggt
    13321 atctgttttt tattgagcgc agcgccttta cccgcattgg tattttcgca ggcggcatat
    13381 tgctgcattc gacagaaaaa acagtcgcca caagcaatga caaacggaat taccacccgg
    13441 tcgccttttt gcaaattttt tacgtccttt ccggtttcaa cgacttcccc cataaattca
    13501 tgaccaaaaa tatcgccatg tttaacctgg ggtattttgc ctcgataaag atggaggtca
    13561 gagccacaga tcgccgttgc cgtaatacgc agaataatat catctgcctg ttcaatgccc
    13621 ggatcgggaa cattttctac ctgaacgtga tgtgggccgt gataagtcaa tgctttcatg
    13681 caacctccag agacagttca aaaaagagtc agtctttaag ggtagctatc acagcgggaa
    13741 aacgggcggt accgcaggtt ttgggagaat tcctggtgtt cagtgagaat atattaatca
    13801 cagaaaccgt tcgtaaggaa aaagaaccat acaaatatca gggataaata ttataatcat
    13861 ggcgaatgat ttgttcatga ttaacaggga gaaagggtta tgtataagac aatcattatg
    13921 ccagttgatg tatttgaaat ggaattgagc gacaaagctg ttcgccacgc tgaattcctc
    13981 gcccaggatg acggagttat tcatctactt cacgtactac ccgggtcagc cagcctgagc
    14041 ctgcaccgtt ttgccgctga tgtgcgtcgt tttgaagagc atctgcaaca tgaagcagaa
    14101 gaacgtctgc aaacgatggt cagccacttc accatcgatc cttcccgcat taaacaacat
    14161 gtccgttttg gtagcgtgcg ggatgaagtc aatgagttgg cagaagaact gggggctgat
    14221 gttgtagtta ttggttctcg aaacccatcg atttcgaccc atctgttagg ttctaacgcc
    14281 tcgagcgtga tccgccatgc caatctgccg gtgctggttg tgcgttaatt gccagaataa
    14341 atatcccgcc ctgcccggag ccatcccggc agggcttttt aatgccataa aaaagccgcc
    14401 aggtttgacc ccggcggcta agcaattgca ggtgaatctt acttcttctt atgcagtttt
    14461 ggtgcgaatc aggtagtcaa aagcactcag agaggctttg gcaccttcgc cagtggcgat
    14521 gataatctgc ttgtacggaa ccgtcgtaca gtcacccgct gcgaacacgc ctttgacgtt
    14581 ggtttcgcat ttcgcatcaa tgataatctc acccatgcgg ttacgttcga ctgcgccttc
    14641 cagccagttg gtgttcggca gcagaccaat ctggacgaaa ataccggcca gttcgatgtt
    14701 gtgaatatcg ccgctgacac gatctcgata ttccagacca acgactttgc tgccgtcgcc
    14761 tttcacttcc gtggtttgcg cattcagaat aatgtcgacg tttttcaggc tgcgcagttt
    14821 gtcctgcaga acctggtcgg ctttcatttc tggcgcaaat tcgagcagcg ttacgtgctc
    14881 aacgataccc gccaggtcaa ttgccgcttc cacgccggag ttaccgccgc cgataaccgc
    14941 tacgcgtttg cctttaaaca gcgggccgtc gcagtgcggg cagtaggtca cgcctttggt
    15001 gcgatactga tcttcgccag gaacgttcat gttgcgccat tttgcaccgg tcgccacgat
    15061 aatgctgcgt gctttcagta ccgcgccaga agctgtttca atctgatgca ggccaccttc
    15121 aaccgctgcc gggatcagtt tgctggcgct ctggctgtcg atcacatcaa cgtcgtattc
    15181 atcaacgtga actttcagcg cacctgccag tttctgccct tcggtcttcg gtacagaaat
    15241 gtagttttcg atatcaacgg tatcgaggat ctgaccacca aaacgttcgc ccatcagacc
    15301 ggtacggatg cctttacgtg cggagtaaat tgctgccgct gcacccgccg ggccggaacc
    15361 gacgattaat acgtcataag catcacgctt gttcagctct tctgccgcac gtttttccgc
    15421 gccagtatca attttggcaa cgatttcagt caacgtcatg cggccctgac caaactcttt
    15481 cccgtttacg aacactgccg gaacgcccat cacgttgcga tcggtgattt cgttctggaa
    15541 ggtgccgccg tcaattgcag tgtgcttgat gcgcgggttc agtacgctca tcaggttcag
    15601 cgcctgcacc acgtccgggc agttgtggca agagagcgag taataggttt cgaattcaaa
    15661 atcaccgtca atatggcgaa tctgctccag cagagactgc gcttctttcg acggatgacc
    15721 accggtccac agcaacgcca gtaccagcga ggtgaactcg tggcccagcg gggagcctgc
    15781 aaaacgtggc ccctggttgg aacctgggtt ggtgatcagg aaagacggct tacgcaccgg
    15841 caagctgtta tcttctttaa aggtgacttt gtctgacagt tctgcgattt cagccaacag
    15901 ttccttgatt tctgccgatt tagcgctgtc atccagcgtg gcaattaact caacaggctt
    15961 ggtcaatttc tcaaggtaag ccttgagttg agttttcata tttgtgtcga gcatgcttat
    16021 ctcctgggct ttaaacatca tcatgcaagc ggcctctgcg gctacctgga tgcagcttgc
    16081 atcatggtgc ggggaagaaa atcgggtgcc gcagcacccg aagaattagg tgaatttccg
    16141 aagtatttcg cgccggagtt ggtagcgcga aagacgaagg aaatttagat tttaccaacc
    16201 aggtccagag acggagccag agttgcttca ccttctttcc atttagccgg gcaaacttca
    16261 cctgggtgag aagctacgta ctgtgctgct ttgattttac gcagcaggtc agacgcgtca
    16321 cggccaatgc cttcagcggt aacttcgatt gcctggatga taccctgcgg gtcaacaacg
    16381 aaggtcgcac ggtcagccag accttcatct tcacgcatgt tgtcgaagtt acgggtcagg
    16441 gcgccagtcg ggtcgccgat catcgcatat ttgattttag cgatggtttc agagctgctg
    16501 tgccatgctt tgtgggtgaa gtgagtatcg gtagatactg cgtatacgtc tacgcccagt
    16561 ttctgcagtt cttcgtagtg gtcagcaacg tcacccagtt cggtcgggca tacgaaagta
    16621 aagtcagccg ggtagaagaa gaagacgctc cagcggcctt cggtatcttt ttcggtgatt
    16681 tcgatgaatt cgccgttttt gaatgcctgg tttttaaaag gtttaatttt ggtgttaatc
    16741 aaggacatct atacttcctc cgtgttttcg atgagatgta aggtaaccaa tttttgccga
    16801 ttcggctaat gcgtttccat tatcaaatcg ataagtttta ccttacaacc ttcgtaagac
    16861 aactttgtga actttgcacc gccagcggcg ataaaaagta aaatggcccc ctttccaggt
    16921 gaccgtgtaa tctgaactac cctttggtat cttcagcgcc tgcctgcagg ctgaggtgtt
    16981 gcccttacaa atgcaacaac gacatggatt acaacaccct cataaacaaa gggcaatcac
    17041 ctgatctaag ctcttaccta tgacagtgat aggttatgcc ttttactcga cttttgcact
    17101 gactgaaaag gacaaattaa tgttaaaaaa gatactttta ctggctctgc ttcctgcaat
    17161 cgccttcgca gaggaacttc cttccccagt aaaagcgatt gaaaaacagg gcattacaat
    17221 catcaaaacg ttcgatgccc ccggaggaat gaaaggttat ctcggaaagt atcaggatat
    17281 gggcgtcacc atctacctga ctcccgatgg taagcacgct atctctggtt acatgtacaa
    17341 cgagaaaggt gaaaacctga gtaacacact tatcgaaaaa gaaatttacg caccagccgg
    17401 acgcgaaatg tggcaacgga tggaacaatc ccactggctc ctcgacggta aaaaagatgc
    17461 gccggtcatt gtctacgtgt tcgccgatcc gttctgccca tattgtaaac agttctggca
    17521 acaggcgcgc ccgtgggtag attctggcaa agtgcaatta agaacattgt tggttggggt
    17581 tatcaagcca gaaagcccgg cgacagcagc ggcaattctt gcctccaaag atcccgcaaa
    17641 aacctggcaa caatatgaag cctctggtgg caagcttaag ctaaacgtgc ctgcaaacgt
    17701 aagtacagag caaatgaaag tgttaagcga taacgagaaa ctcatggacg atctgggggc
    17761 aaatgtcact ccggctatct attacatgag taaggaaaat acgctacaac aggccgtggg
    17821 gttgcccgat cagaaaacgc ttaatatcat tatggggaat aaataaaagg taattcatgg
    17881 atggttgaac tatatccctg cttttcaacc atcctaaaaa agcatgaatt gctcgctctg
    17941 ccctaagtgg ctgtgctatt ttagaaaaac ttaatcctga gttaagcaaa aaaacataat
    18001 caataaaaat atatacgcaa aaaacattga ttaagtgaat atatcatgga agaaaaatat
    18061 aaccggagta gtgtatggcc aatctctacg acttgaaaaa gttcgactta aatcttcttg
    18121 tcatatttga gtgtatttac cagcatttaa gcattagcaa agctgctgaa tcactatata
    18181 tcaccccatc agccgtgagc cagtcgttac aacgcttacg tgcgcaattt aatgaccctt
    18241 tattcattcg ctcaggaaaa ggtatcgcgc cgacgacaac agggttaaat ctgcatcacc
    18301 atcttgagaa aaatctcaga ggccttgaac aaacaattaa tatagtcaat aagtccgaac
    18361 taaagaaaaa ctttattatc tacggccctc agctcatttc ttgctctaat aatagtatgc
    18421 tgatccgctg cctacggcaa gattcctctg tcgagattga gtgccatgat atcctcatgt
    18481 cagcagagaa tgctgaagaa ctgttggttc accgcaaagc cgatctggtg atcacccaaa
    18541 tgccagtgat cagccgttcc gtcatttgca tgccattgca tacgattcgc aatactctaa
    18601 tttgtagtaa taggcatcca cgaattactg acaatagcac ttatgaacaa attatggcag
    18661 aagagttcac ccaattaata tctaaatcag caggggttga tgatattcaa atggaaatag
    18721 acgagagatt tatgaatcgc aaaatctcat ttcgtggttc ctctttgctg acaatcatta
    18781 atagcattgc agttacagat ttactgggaa tagtccccta tgaactttat aattcctacc
    18841 gtgactttct taacttaaaa gagataaaac cagaacatct gttaccttca ataaagcttt
    18901 acatttccta taataaatct tctttaaata atttggtgtt ctcccgattt attgaccgtc
    18961 taaatgagag tttttgatat ataactacag acagctacta taactttcat ccatttattc
    19021 acagcgcgaa tgaaaaataa aacactctgc tttacagtgt ttactttata attaacagac
    19081 ttatctcact gatcaccctg taacgttcag agagcgtctt cagatgtcta tttataaaat
    19141 tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga cactcaacac
    19201 cctaccccgc gtttgtgtct cattttctgg cggcaaagac tctggtttaa tgctgcatct
    19261 gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta tcgactggga
    19321 ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt acaccgatgt
    19381 catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc tttcacaata
    19441 ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtacgtcaac ctccccaaga
    19501 tgcgataacc gaccctgact ttttctcctt ttaccagcca ggcatgacct tcgaacaatt
    19561 tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gcggcgatga tgatcggcat
    19621 ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata aacaacgttt
    19681 tgccgacgat aaaccctgga ctacggccgc accaggcggt catagctggt acatttaccc
    19741 catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc aaagtctctg
    19801 caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata tgcgaatttg
    19861 cgaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta tcgaaccgga
    19921 tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca tttacgccgg
    19981 acatgacaat catttctatg ggcatcggaa aatcctcaag ccagaacatt tagactggca
    20041 agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc attaccgcaa
    20101 taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc cacaaaccca
    20161 gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca aagttttact
    20221 caataacgat tactggtgtc gggcattatc atttagcccc acaaaatcga agaactatca
    20281 gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat gcaacaacga
    20341 ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg catcaaagcg
    20401 attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga gccagtggat
    20461 tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc aaacaacgtg
    20521 gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt tacccaaccc
    20581 attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt tcaccgccat
    20641 gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt gccggtgacc
    20701 tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa tcgcgctcgt
    20761 ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca gttgggatgg
    20821 gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct gcgcctgaag
    20881 caaattaacg gcctgcaaga gttgtttgcc gaccgtcaat attcccgcgc ctggacggtt
    20941 aaatagctaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt ccttcttggc
    21001 aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg acagcggaat
    21061 cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat ccagggtaga
    21121 aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa tctccagccg
    21181 gctttcattt aaagcattca ccagaatatc gcgcttctgg cgataaaagt ccggtaacgc
    21241 aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg ccggggtatt
    21301 caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg gcgctggcgc
    21361 aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg aagaaaccgc
    21421 cactgcccgc tcacgcagct gcggatgcgc cagcacactg gcatggccct gttgtgaaaa
    21481 gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg cgatcgcctg
    21541 ccacaaagcg gcgaaatcag cctgctgcca gatagttgca ctggggttat gcggagtgtt
    21601 gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc agtcaacgcg
    21661 aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg cgatggcggg
    21721 ggagtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc gcaccagtgc
    21781 ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat cgctatcggc
    21841 atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc gcaaggcctg
    21901 cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg ccagccgctc
    21961 ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa tcgcctggtg
    22021 ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg gaagtttgct
    22081 ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg cgtgttgaag
    22141 gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta aataaaacaa
    22201 taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc ggctaactac
    22261 ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga acaactttct
    22321 cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa acttccgcca
    22381 gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag cgaaagcgat
    22441 gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg cgtcggcggc
    22501 ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc atttgttgcc
    22561 gctccgacga tcgccgccac ctgcgcagcc tggacaccgc tctccgtctg gtataacgat
    22621 gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt gctggtggaa
    22681 ccggagatta tcctcaatgc accgcaacaa tatctgctgg cggggatcgg tgacacgctg
    22741 gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc gctaaccgtg
    22801 cggctgggga tcaataatgc gcaggccatt cgcgacgtct tgttaaacag tagcgaacag
    22861 gcacttgccg atcagcaaaa tcagcagtta acgcaatcat tttgcgatgt ggtggatgcg
    22921 attatcgccg ggggtggaat ggtcggtggt ctgggcgatc gttttacgcg tgtggctgcg
    22981 gcccatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt tctccacggc
    23041 accaaagtcg cttacggaat tctggtgcaa agcgccttgc tgggtcagga tgatgtgctg
    23101 gcgcaattaa ccggggcgta tcagcgtttt catctgccga ctacgctggc ggagctggaa
    23161 gtggatatca ataatcaggc ggagatcgac aaagtgattg cccacacctt gcgcccggtg
    23221 gagtccattc attacctgcc agtcacgctg acaccagata cgttgcgtgc agcgttcgaa
    23281 aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc cgccttttcc
    23341 accgtagcgc gcgtcctgcc gctcacggaa aaactcctcg taggtcatcg gcgtttgatc
    23401 gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac caatcatcag
    23461 cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca tcttggcctc
    23521 cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgctttatc cggcctacaa
    23581 atgctatcaa cgcactcggt cagtcccctc gccccatcgg ggagagggtt agggtgaggg
    23641 ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga ctttagtgcg
    23701 cacctttcgc ctgcgctacg atctcctcaa cattttccgg cattggctca tacggcgttt
    23761 ctttcgccgt tggcttcggc tctttcaatg ccgcaagtgc cgtcttaatc gagaacagtg
    23821 ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga cggttgttga
    23881 acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg ctgtcgatca
    23941 ttgcctggaa cttgttagca atggccagga agccgacttt cgcatccggg ctaaacgctt
    24001 tctgccagcc tgcggtcagg gtacaaatca gtagccaggc cgttggtacc agcgccaccc
    24061 aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc agcgccatcc
    24121 ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaata ccgcccagcg
    24181 gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc gccgttgcca
    24241 gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg cccagcagat
    24301 cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga ataaacagag
    24361 cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca cccagcgcac
    24421 cgtgcaggat gtaagccatc cccaccgcca acgtcggcgc accgcccgca cgggaaatga
    24481 tcgactgttc acccacttcg ctggcaatct ggttgagcgt atctggtgta atgctaaagc
    24541 cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct gccggagcca
    24601 gcaccgccat tgggctgttc atggcaaagt aaacgcccgg atcgatgata caggcggaaa
    24661 ccagcgccat aatcgccacg aaggattcca tcaacattcc accgtagccg ataaaacaag
    24721 cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc gcatggaagc
    24781 cagacaccgc gccacaggcg atggtgataa acaggaacgg gaacaggtta ccggtccata
    24841 ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc gggcgcataa
    24901 tcaaaatgcc taccgccaga ccgacgatcg tcccgatttt caggaaggta gagaggtagt
    24961 cacgcggggc cagcagcaac cacaccggca gcaccgccgc cacaaaaccg taacccacca
    25021 gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc ggactttctg
    25081 ccacccagcc gccagagata atggcgaaaa tcaggaatac cagaccgatg accgacactt
    25141 caccaatacg ccccggacgc agatagcgca ggtagatccc cataaacagc gccagcggaa
    25201 tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc accacgatca
    25261 tcgccagtac tgcaaggata atgaccatga tcataaagca ggccaccagc gctatcaccc
    25321 cggcggttgg ccccatctct tctttgacca gctcacccag cgatcgaccg tcacggcgcg
    25381 tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc ccggcgagca
    25441 gccagatcat ccctggcagg taacccattt gcgccgccag taccggccca accaacggac
    25501 ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc gtcggcacat
    25561 agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca accgccagca
    25621 catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata cagaccgacg
    25681 ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg tatcccagag
    25741 caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat ttgttcatag
    25801 ttgttatccg tgtgcgtgtc catagatatg ttacattttg tttctataac atctttgtac
    25861 gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa cttaacaatg
    25921 ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc tgtcgggctc
    25981 atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt tcatcgaaaa
    26041 cgacgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag acgccgcgta
    26101 ccttgccttc agacaccggg cgatgatggg tggcgttaag ttccgtaccc accacacact
    26161 gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc agcgccgctg
    26221 acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc ggcatttcgg
    26281 cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc accattgtgt
    26341 tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc atgcttatgc
    26401 ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc tggcgaggtc
    26461 agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt tccccagcgg
    26521 aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg cgtcatcgct
    26581 cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca cattacagcg
    26641 cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca gcgccgcttt
    26701 cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg acgccacagt
    26761 gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa acaggttaaa
    26821 cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttgctca gttggtctgt
    26881 cgcgcccatg cgtaaaattc ccgccgcatt gaccagcgcg tccagtcgct ccgtttcagc
    26941 taacagtcgc tgacacactt gcgccacctg cgcagcgtcg gcaacatcca tcacttcggt
    27001 cgcaaaggga tattgctcct gagtgaacgc ttgatcaaaa cctgtaactt tcgctcccgc
    27061 ctcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg ttacccagac
    27121 atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca ccaggcgtcg
    27181 atggtcgggt ttttcgccag catgacaaaa tcgatatcac catgcacttt gcgccagcgc
    27241 gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt gtcgtcatcg
    27301 aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc cgctttgctg
    27361 gcggggacag gtgctggcag taattcttca gtcatcacca cccggccaga ccgcccggcc
    27421 acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc atccgccacc
    27481 ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca gccaatgtgg
    27541 gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat ttgctccagc
    27601 ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc ggcatctggc
    27661 gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg cccccacata
    27721 tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt gtaataaacc
    27781 gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat cacctgctcc
    27841 atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc atggattaac
    27901 aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat atcgtgagac
    27961 tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc ttcaggctga
    28021 tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga ccgccgtcag
    28081 cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa taccctgttc
    28141 acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca gataagcaca
    28201 gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga tcaccgccgg
    28261 atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc cacggttgat
    28321 ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag agatcagatc
    28381 gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg gacttttgta
    28441 ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc cttgcggcag
    28501 tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg ggtaaccctg
    28561 ggtatggata attttctccg cgctatcatc aagacgggtg tagttcacca gcccttccgc
    28621 catgccaaac acctgctgca actggcagcc aatctcagcg ggaatacgcg ccgcaagggt
    28681 ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct gcgcccggct
    28741 ttcaccttcg gtcagcgcct gcaaccacag gctgactgcc ggtggcacca gcgcggtgac
    28801 gttcacctga tgtttttcaa tcaatgggaa gcaaagcgtg gcgctgggat cggccgccag
    28861 aacaaccgtt cctccggcaa gaaagacgcc cagcgatccc ggcgaactca tggcgtagtt
    28921 atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac aaatctcgac
    28981 gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagtt tcggtgtgcc
    29041 ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg gcgtggcagt
    29101 aaaatcctca gccggatggt taatcgcatc ctgcaagtta tgctcaccgc tgtcgttgtg
    29161 cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga aatcatcccc
    29221 gctaaacagc gcatgttggc gatcggcaat cagcaatgcg ggttcaatct ggctggcata
    29281 ggcgttcagt tcactacgct gatggctgaa cagcgccagc accggcgcaa cgcccagttt
    29341 cagcagcgcg aaaaaggtaa tatacaattc agcgacgtta cccagttgta ccagcgcggt
    29401 ttcaccaggt ttaatgccct gacggcgtaa actacacgcg aggttatccg ccgcctgatt
    29461 cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt cactcgcagc
    29521 gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt cccgatagcg
    29581 acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc cttaatgcaa
    29641 tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact cacccaacgg
    29701 tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt cccgcagctt
    29761 cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc aacccacaat
    29821 gccgccaaac agttcgcggt cgaatggctc cagttcagca ataacctggg tcgcggcctg
    29881 atgcgggaaa ccgctcagcg cgggggttgg atgcagcaga caggccagag tcagtgcgtt
    29941 ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg tcggcgtggt
    30001 aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca gtacctcttt
    30061 catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca gcagacgatt
    30121 acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg ctaacggaat
    30181 ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc ccagcaggac
    30241 gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg caatcaaccg
    30301 ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca atcgtgacaa
    30361 caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa ccatctgttc
    30421 aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact ggctgcgggt
    30481 aaaacggcgg gctgaggtct gtttttcctg gcgggagaac gactgccagg attcgggaat
    30541 atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca ccggattttt
    30601 gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga agggactgtc
    30661 gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg acgtcgtaaa
    30721 actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca tggtctgctg
    30781 tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa ggctttaata
    30841 atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact atgtcaactc
    30901 ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa tgaaaatgag
    30961 aagcattatt gatggattcg cataagcgca atgtgatgtc ctgcgccgtt ctgccccctc
    31021 tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct ggctgttccc
    31081 ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt gtcgaatttg
    31141 tcattacgcc cttaacctta ttaataacag gaagctgatt tgtgagactc gccccgctct
    31201 accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgca gttcaggccg
    31261 ctgactggcc gcgtcagatt actgacagcc gtggcacaca tacactggaa agccagccgc
    31321 agcgtattgt ttccaccagc gtcaccctga ccggctcact gctggcgatt gatgcaccag
    31381 tgatcgccag cggcgcgacc acgccaaata accgcgtcgc ggatgaccag ggctttttac
    31441 gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc ggcgaaccga
    31501 gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca actggcgggg
    31561 attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta atcatcaatt
    31621 acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc gggcatgaga
    31681 aacaagcggc agagcggatt gcgcagtttg ataagcaact ggcggcggcg aaagagcaaa
    31741 tcaaattacc gccgcagccg gtcactgcca ttgtctatac tgccgctgca cacagtgcca
    31801 atctctggac gccagaatca gcacaagggc agatgctgga acaactcggc tttacgctgg
    31861 cgaagttacc cgcagggtta aatgccagcc aaagtcaggg taaacgccat gacatcattc
    31921 agcttggtgg ggaaaatctg gctgcagggt taaatggcga gtcactattc ctgttcgccg
    31981 gtgatcagaa agatgccgat gctatttatg ccaatccact gctcgcgcac ctgcccgcag
    32041 tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt ccgtctggat tactacagcg
    32101 ccatgcaagt gctggaacga cttaaggcgc tgttttaagc attaaccgtc agacgctgtc
    32161 acctgcggcg gcgtctggcg aaaacgtcgc aactccacca gcaccagcaa taacaacacg
    32221 ccgataatca acaaaccaaa accgctcgcg cttgcggaag caaccggtgt catcatcgct
    32281 cccaaaccgc ccagcagcgc cgcgcctatg gcatcgcccg tcacgttttg tgccgtccac
    32341 aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg tttgcagcat tgtgtattgc
    32401 agcaacgagc tgaccgcact caaccagccg aacagcgcca gacaaaccac gcctaaaatc
    32461 cacatcggca tcaggccaaa cagaccaatg gcgaggaacg atcccagcgt ggagagcagc
    32521 atcaataacc ctggtcgcgc gctatgcgcc agcttcccgc tggttaacgc gccaatagcc
    32581 gcgccgagcg ggatcgccgc gtagagaaaa ccaatctttg ccgctgacat ctgccagttg
    32641 tcagccagcg ccggatacag tacccgcacc gcgctcgcca tcgtcaataa cccccccagc
    32701 agcgcaatgc ctcccaccag cgggctggcg agcagaaaac gaaatcctgc cagtaatgat
    32761 ttcaacggat gctcacgcgg ctgcggtggc ggtggcagtg ccggaaggct taacaacggt
    32821 agcaaggtaa taaacgtgcc cgccgccgcc agtccgtagt tccaggccac accgccagtc
    32881 gccagcagta aaccgccaat catgggcgaa atcaccgacc ccagacgcac ggtcaacatg
    32941 gtgatcgccc cggcctgcat taagttttca cgccctacca gtgcaggtgt cgccgccagt
    33001 agcgccgtaa cgccaagtga tgcgaaaaaa ccatcccata aaccaagtaa atagattgcc
    33061 agcaatgacg gttcaggcag cagtgcatta aggcacagtc caatgaagcc aatgccacag
    33121 gtgccgcgcg ccagcaaaat cacttttttg cgttcatagc gatccgccag cacaccgccg
    33181 accatcaggc caacaaacat cgcgccgccg gtcagcgtca ccgaaagccc cacctgccag
    33241 gtagaatgtg tcatcatctg gatctgcacc ggcaccgcga cgccgagcaa acccagagaa
    33301 acaatagaga tgaaacgagc gaggaatact gcgcgaaacg ccgggtgcgt tttcaacagg
    33361 ctgaggttaa gcagccaaga ttgtttattc attacaatgc cttgccatca aatattttaa
    33421 atacctattc ttaggctgca catgctaaca tatccaaata agatcgataa cgataattaa
    33481 tttcattatc atggaagttc gtatgtctgg ttctgttgcc gtgacacgcg ccattgccgt
    33541 gcccggattg ctgttattac tgattatcgc gacggcatta agtctgctca ttggggcaaa
    33601 atcactcccc gcttccgtag tgctggaggc cttctccggc acctaccaga gcgccgactg
    33661 caccatcgtg ctcgacgcgc gtctgccgcg tacccttgcc ggtttactgg caggcggcgc
    33721 gcttggcctt gccggggcgt taatgcaaac cctcacccgt aacccacttg ccgaccccgg
    33781 cttgcttggc gtgaacgccg gagccagctt tgccattgtg ctgggtgcgg cgctgtttgg
    33841 ttactcttcc gcacaggaac aactggcgat ggccttcgcc ggggcgctgg tggcctcgtt
    33901 gattgttgcc tttaccggca gccagggcgg cgggcagtta agtccggtgc gtttaaccct
    33961 ggcgggcgtg gcgctggcgg cggtgctgga aggactgacc agcggcatcg ccctgcttaa
    34021 tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt tcgctggata ttcgcaatct
    34081 acatacctta aaagtggtgc tgatcccggt gctgatcgcc ggagcaactg cgctattact
    34141 gagtcgcgcg ctgaacagtt tgagcctcgg cagcgacacc gcgacggcgc tgggcagtcg
    34201 cgtggcgcgc acacagttga ttggtctgct ggcgattacc gtgctttgtg gtagtgcgac
    34261 ggcagtagtt ggcccgattg cctttattgg cctgatgatg ccgcatatgg cgcgttggct
    34321 ggtgggtgcc gatcatcgct ggtcgctgcc cgtcacgcta cttgctaccc ctgccctgct
    34381 gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggc gaactgcgcg tttctgtggt
    34441 cagtgcgttt attggtgcac cggtgctgat cttcctcgtg cgacgtaaaa cgcgaggtgg
    34501 tgcatgattt acgtctctcg ccgattactc atcacctgtt tgctgctggt ttccgcctgt
    34561 gtggttgcag gtatctgggg attacgcagc ggtgccgtca cgctggaaac ctcgcaggta
    34621 ttcgccgcgc tgatgggcga tgcgccgcgc agtatgacga tggtggtcac cgaatggcgt
    34681 ttaccacgcg tgctgatggc gctgttgatt ggcgcagcac tgggcgtcag tggcgcgatt
    34741 tttcagtcgc tgatgcgtaa cccgctcggc agccctgacg taatgggctt taacaccggg
    34801 gcgtggagcg gcgtgctggt ggcgatggtg ctgtttggtc aggacctgac ggctatcgcg
    34861 ctgtcagcaa tggtgggcgg cattgtcact tcgctgctgg tctggctgct cgcctggcgc
    34921 aacggcatcg acacctttcg gttgattatt atcggtatcg gcgttcgcgc catgctggtg
    34981 gcctttaata cctggctgtt gctgaaagcg tctttagaaa cggcgctaac agcaggtttg
    35041 tggaatgccg gatcgctcaa cggcctgacg tgggcaaaaa cctcgccttc cgcacccatc
    35101 attatattga tgctcattgc cgccgcctta ctggtacgcc ggatgcgctt gctggaaatg
    35161 ggcgatgata ccgcgtgtgc gctgggcgtc agcgtcgaac gttcgcgtct gttaatgatg
    35221 ctggttgcag tggtgcttac cgctgcggca acagcgcttg ccgggccgat ttcctttatt
    35281 gctttagtcg caccgcacat tgcccgacgc attagcggca ccgctcgctg ggggctaacc
    35341 caggcggcgc tatgcggggc gctgttactg ctggcggccg atctctgcgc ccaacaactg
    35401 tttatgccgt atcaacttcc ggttggcgtc gttaccgtca gcctcggcgg tatttacctt
    35461 atcgtcttgt taattcagga gtctcgcaaa aaatgaccga atcagtagcc cgtttgcgcg
    35521 gcgaacagtt aaccctcgga tatggcaaat ataccgttgc ggaaaatctg actgtagaaa
    35581 tacctgatgg tcacttcacg gcaattatcg ggccaaatgg ctgcggtaaa tccacgttac
    35641 tgcgtacctt aagccgcctg atgacgcctg ctcatgggca tgtctggctg gatggcgagc
    35701 acattcaaca ttacgccagt aaagaggttg cacgccggat tggtctgttg gcgcaaaatg
    35761 ctaccacgcc gggcgatatc accgtgcagg agctggtggc gcgtggacgt tatccgcatc
    35821 aaccgctgtt tacccgctgg cgcaaagagg atgaagaagc ggtaacgaaa gcaatgcagg
    35881 ccacgggaat aactcatctg gcagatcaaa gcgtggatac cctttctggc ggacaacgcc
    35941 agcgcgcgtg gatcgcgatg gtgctggccc aggaaacggc aattatgctg ctcgacgaac
    36001 ccacgacctg gctggatatc agtcatcaga ttgatttgct ggagttgtta agcgaactga
    36061 accgcgaaaa aggctatacc ctggcggcgg tgctgcacga tcttaatcag gcctgtcgtt
    36121 acgccagcca tttgattgca ttgcgggaag ggaaaattgt tgctcaggga gcgccgaagg
    36181 agattgtcac tgctgaactg attgagcgca tttatggtct gcgctgcatg atcattgacg
    36241 atccagttgc cggaacgccg cttgtggtgc cgctcggacg aacggcacct tcaaccgcaa
    36301 atagttaaac taagtggtct gccatcatgg catcctgttt tctggatgcc atcgcatagc
    36361 gcaataacac gctaccacaa gccaccatcc cgccgatcag cgcggaaagg atcacaataa
    36421 tcgccttacc cggaccgtct tttttcactg gcaatgacgg gcttaactga tatttaaacg
    36481 gcgtaaaatt cacatcgttg atatttgctt ttgttaactg ctcaacaaga tactgacgat
    36541 tacgtaattc accgttcagt tccgcaacgt cagtgaccgc tttttcaatt tccagtttgc
    36601 gttcaatacc gtctgcgccg agagaaatag agaaatcggg gtcatcttta acggcctgac
    36661 cattactgta tacgggcttt ttaattcccg ccgcgttggc aatgtcgagt gaataattga
    36721 ggcgctgaat gtttgcatca agttgatttt tcgttttaat gcgatcctga gccagttttt
    36781 ctttttcaaa ctgggttttg atttccagtt tattacggac gttttctagt gactctttca
    36841 ccaccaacgc agagatataa tcgatatacc cgctcaaaac ggtctgcgcc tcttcactgg
    36901 ttggcgcggt aaaacttagc gtccaggagg tatacagtga cggttcatct tttttcttac
    36961 tggcattgtc atcaaccgct ttcatttttt cgctcaatgc aacaatagcg cgatgcaaat
    37021 ccagttcatc gattttcgcc tcttttaatt ggtccatcac ataaggtgat gaacgcaggt
    37081 actcttccag caagctaacc gactgaaact tcttgataaa caggttaaat gcttctgtac
    37141 gatcaatttt gatatccaga tccagcactc gaagcttagt gaatgttttc tccagctctt
    37201 gccactgaac aggttctgga ggcgtgacaa ccgccgcgct ggtccatttt tgcggcagga
    37261 tgaaagagat cagcaagcct gcgcaggcaa acgcaaaaat gaccgccatg accgtttttt
    37321 tggcccgcca taaaactgcg attagattaa gtaaatcaat ttcattatta ctgggcgacg
    37381 ccagaggata ctcaggaaaa tgagcgtcac ttccctgttt aatattcagt gatgacatgg
    37441 ggtataacct gaaaatgagt ccaatagggg aaatctccac ggcgctcact ttaccagttg
    37501 gtgataatta tccgaagctg aagtttgtaa attccttcca ctgaatgcaa cattcactct
    37561 atttaagaga aatattcatc aaacctataa atcaaacaaa taaaaaaacc cataattatt
    37621 ttctgtaatt atgggtttat agatattatt aatttacctg tttagcgttg cgcgaataat
    37681 cggcccaatt tttacaaacg cccctggaga gataatatcc acatgcgcac aatcctgacg
    37741 atagatatcc agctcggcta tccacggcga ccaggcgcgt tcggggctca taccttcctg
    37801 aagcgtgcgt tcagcaacaa acagcgtcgc tttaccatca aacggtacgc tatgagcagt
    37861 cgtcagcagg cgcacagcat cagcgtagtt gccttcaatg gtggtaaaca actccgttga
    37921 agtacttccc tgctgtgccg ccaggaaggc ctcgcgctcg cggttaatct ccgccagcac
    37981 ttccgggtcc agaccattag cctctttttc ctgccagttt tgcgtttctg gcggccaggt
    38041 atccagcaag ccaagaaatg ccacctgttc gccacgggca cgcagtcgcg ccgcaatgcc
    38101 ctgcgccagc gtaccgccaa gggaataccc cagcaggtaa taagggccgt gcggttgttg
    38161 ctcaagtagc gttgccagat gcgcttcgca gacttcatcc aagtttgtcg ctatctgcat
    38221 ggggccatga gggcgcggtg actgaatgcc gataatcgac cattgtggat cgagataacg
    38281 cgagagcacg ctgaactgcc aggcaaaacc ggacgcagga tggaaacaaa acagcgtcgg
    38341 gccattacct tcacgcaacg gcagaatggt ttcgaatccc atgcgccggg cgctgtcttc
    38401 ttcaccatca ataatcgttg ccagtttggc gacggttgac gcgaccatca cctgccccgg
    38461 cgtcacctga cgagcaaact gtcgacttaa ctgcgctgcc agtttcattg ccagtagcga
    38521 atgaccgcca agcgcaaaga aatcagcatc ggcatcctgc acgtcacaac ccagcaacga
    38581 cgcgaatgcc gcggcgataa tcttttcact gcccgctttc ggtgcacgtc ccgacgcttg
    38641 tgtcttcagt tcaggcaacg gtaaggcttt gcgatccagc ttgccgttgg cgctaagagg
    38701 taactgtgga agttgcagca gaaccaccgg caccatatgc ggcggcaatg tttcgcgaag
    38761 ctgtgcctgt aatgcgctgg tatccaacgg cagacctgat tgcgacacca gatagcccac
    38821 caactgacgc gcatcaccac cggtggctgc cgccaggtta atcacacagg cgtgggtaac
    38881 ggcttgtttg acatccggca gcgcctgcat cacgcgatcg atttcgccca gttcgatacg
    38941 ctgcccgcga atttttagct gatcgtcact gcgcccgagg tactccaccg cgccgttatc
    39001 cagccagcgg gcgacgtctc cggtacgata catccgttca cctggggcaa aaggatcggc
    39061 aataaaacgg ctggcggtca gatcgggtcg tccaagatac ccctgcgcca gttggatgcc
    39121 ggtgagatag agatcacccg ccacacccgg cggcaccgga tgcatcatcg catcaagaat
    39181 acgcagaccc gtattccata ccggataacc aatcggcaca ctgctgccgc gcacctgtgc
    39241 cagttcctcg ccaaaagccg gataccagct gacatctacc gccgcttccg tcgggccata
    39301 caggttatgc aacggcgcgc cggttaactg ttgccattcg cggcataaat cagccggtaa
    39361 ggcctcacca ctacagaaaa cctgtttcaa cgtcgcgcaa ttctggcgag cggtttgcgg
    39421 cgtcagcgat gcaacaaatg ccgccagcat cgacggcaca aagtgcgtgg tcgttacgcc
    39481 atattcggca aagaattgct gcatagcgag cgggtcacgg tgcgcttccg gttcagccat
    39541 caccagtttt gcccctgcga taaacggcca gaaaaactcc cacaccgaga catcaaaact
    39601 gcacggcgtt ttttgggcaa cgacatcttc gcctgtaagc gggtagtgat tttgcatcca
    39661 taacaggcgg ttaacgatag ccgtctgccc gaccattacc cctttcggcc tgccggtgga
    39721 gccagaggta aagatgatat aagccgtgtg gtgcggttgt gaaagttgca gcggcgcact
    39781 gccctggggg gtaagcgggg cgttatagca aaggcttgtt aaattgggaa catcgctaaa
    39841 gcgcggcagt tgatcgtcgg tggtaattaa cagcgacgga cgcgcatctt ccagcatcat
    39901 tttcaggcga tcgtccggat agccggtatc cagcggtagc caggccgcgc cagcttcaac
    39961 tattgcatgg agtgccaggg tcaaaaagac cgagcgcggt agtgccaccg ccacgctgtc
    40021 ccctggttta acgccgcgct cacgcagcag attcgccagc gccaccacct gctcgcgcat
    40081 ttcccgatag ctgaacaggt aacgcgcatc tgccagcgcc ggagcatccg gtgtttttgc
    40141 cgcttgttct gccaccagcg cgctcagcgt ggtttctgga atctcaacct gagtggcgtt
    40201 gatctgcgcc agctgcgcat actcacctgg cagcataata tcgacatcgc cgcacaacag
    40261 cgccggatcc gcggcgaact gggcaatcag cattttcagg cgttcagcat gctggattaa
    40321 cgttggctca tcgtaacgct gtttattggc gaggatctca atactcaaat caccgtgttc
    40381 atccgggaac agggccagtt caaggtcatt aaccggaccg gttgccaggg tatgggtttg
    40441 cgtctgaaca ccaggaatat ccagttggta atcaaatacc ttgatattga gtaccggacc
    40501 gaacagcggt tcctctcctg ccgctcgccc gctgtcacgg acaatttgtt cggcatcgta
    40561 acgttgatga cgacgcattt ttttcagttg tgctgccagt cgggttgcca gttccggcag
    40621 cgtttcttgc gccgcaatgt gaatacccaa cggtaaaacg ttgagcacgg gtccgctagc
    40681 cgtcagcgcc gccgagccca gtcgacgcat aaagataaat ccggcggcat agtccatgcg
    40741 attgcacaat cgccccagcc acaaggctgc cagcgcaagg gctaaatcgg tacgctgcac
    40801 acctgaaagt tgcgtagcca gctggcggaa ttccccgtcg gtaaattcca gtttcaggcg
    40861 cagaatatct gccgaagcgc tgcgccccgc taaaggcgcc ggagaaagtg acgcgggcgg
    40921 cggcagttga cgacgctgtt ctgcccagaa tgccgcatca cgctgccagg cttcgctttc
    40981 gcggtattgc tggtactctt ccactacatc agcgaaaggc gtaaacggcg aagcaggcgt
    41041 tggttcgcca cgcagccatg tgcagtaaat attggcgatc tggcgagtaa tggccgggaa
    41101 actgaagcca tcgaccagca aatggtgata acgctgatac cagtaccagc ggttatccgc
    41161 aacatgtatc agctgatgaa agaccagtgg tttaccgcta tcgacgcgca aatcttgttg
    41221 caaatccgcc agcattaatg cctgcgcagt accgtgcgga tcaatattgg ttcgcaggtc
    41281 gataatttct ggcagttcga atatcagcgc atcatcgacc cactgccaga cttcgccgtt
    41341 atcttccgta aaacgcatcc gcaacgtatc tgcttgcgct agtccgacaa ccaccgcgcg
    41401 ggccagtaat ggcgcatcaa catctccagt taactcaacg taatgcgcca cgctccaggc
    41461 ggagggtaaa tccgacagtt tttctgccat ccagatgccg ggctgtgcgg cgaccaaagg
    41521 taaatgctgg ctcattgtgc ctcctgcaac tgggtgaaat tggccggtgt cagagtacgc
    41581 cagtggactt ccagccactg ctggcaggac gcctgtgact gcggctgaca cacaatgtcc
    41641 cagcctgccg gtaaggcgca ttgttgcggc cacagactga attgcccctg cgcattgcgc
    41701 aaaatgtaaa acgctccctg tggatcatcg aagggattac tgaatgccat attcaactcc
    41761 tgtcatggaa aagtggttgc cagaggtcga ttagcccctg catcaagcca ccgcgccaac
    41821 aaagcgcatc atgtccgccg tcaacctgac gccagaaaat ggattctttt atggggtgta
    41881 attgcgcata cagcgcctga ttggcccgca taatcatcgg ctcgcgaata cccgcttcca
    41941 gcacaatgcg cagaccttcg gcgctaactt caccagcttt aagcttttca agtaacacgc
    42001 cctcttgctg cccgccccga tgcggccacc agtacgatcc tgactggctt aatacacagc
    42061 caaagcgttc aggccagtgc agtccggcat acagcgcgga cagcccacca aaactctgcc
    42121 cggcgaccac ggtgcgatcg gcgcgatcgc taaaaggggc aatagctttc accaggggta
    42181 ataactcttg ctgtactgcc agccagaaat ccgcattaca cggcagttcg tgggcgcggt
    42241 gcgtggtgtc gatagcgtcg atcaacacat acacggcggg aggaagttgc tgacgatggg
    42301 tcagcgaagt cagcactggc cagacgggca tactttgcgc ccaaaattcg ccatcgagca
    42361 aaactgccag cgggcgttct tctgctgttg catcgccggt ggtaaaaatc catacacgcc
    42421 gtgacttttt caaccgttca cttttccaga taatttcttt ggcaggtatt tctggcgctt
    42481 gcggacaatc ccatcccggt tgcagaggcg cttgcggcat ttcgagtgca gaaacagcgt
    42541 gccctcgccc gcctttccag ctttgtaggt tcagcggatc ggctatcgcc tggggtaata
    42601 gttttcgcca gccttcgcgc aattcgaggc gatcggggga tggtacagaa aaaatgtcat
    42661 cgcgttcggt gggaataaag cagtagctgc cgcgccagtt ggcattgagt tgtgtcttcc
    42721 actgccagac gttagtgcct gcaattcgct gcatcgactg gggctggctg ttctgatggt
    42781 gatcggtcac accagtgatg tagacccata cgcgctttat cgtcgagtat tcttcagaac
    42841 cttggggatc acgccaccag aaagtgacct caaacatttc gtcattcaga cgctgccatt
    42901 ccgggccatg tttcgactgc caccagctct cacttcctac ttttaacgcc gtcacaccat
    42961 aaccccatgt ttactgtgca atttttcatt gattgcagaa atatattgat aatattattg
    43021 ataactattt gcatttgcaa tagcgtaatg gcgcgccgtg ggaagcgcgg acattaatta
    43081 accaactgca ctgcgtgtct ttcaggatca aaggttttcg cggtagcggg atgcgtcgtg
    43141 ttgatgacga ccatgcccga cagttgcaat tcgtggcaaa aatgcaggaa taaaacaatg
    43201 aacaagaaga ttcattccct ggccttgttg gtcaatctgg ggatttatgg ggtagcgcag
    43261 gcacaagagc cgaccgatac tcctgtttca catgacgata ctattgtcgt taccgccgcc
    43321 gagcagaact tacaggcgcc tggcgtttcg accatcaccg cagatgaaat ccgcaaaaac
    43381 cccgttgccc gcgatgtgtc ggagatcatc cgtaccatgc caggcgttaa cctgaccggt
    43441 aactccacca gtggtcagcg tgggaataac cgacagattg atattcgcgg tatgggtccg
    43501 gaaaacacgc tgattttgat tgacggcaaa ccggtaagca gccgtaactc ggtgcgtcag
    43561 ggctggcgtg gcgagcgcga tacccgtggt gatacctcct gggtgccacc tgaaatgatt
    43621 gaacgtattg aagttctgcg tggtccggca acggcgcggc gggcggcgtg gttaacataa
    43681 ttaccaaaaa aggcagcggt gagtggcacg gctcctggga cgcttatttc aatgcgccag
    43741 aacataaaga ggaaggtgcc actaaacgca ccaacttcag cctgaccggt ccgctgggcg
    43801 acgaattcag cttccgcttg tatggcaacc tcgacaaaac ccaggctgac gcgtgggata
    43861 tcaaccaggg ccatcagtcc gcgcgtgccg gaacgtatgc cacgacgtta ccagccgggc
    43921 gcgaaggggt gatcaataaa gatattaacg gcgtggtgcg ctgggacttc gcaccattgc
    43981 aatcgctgga actggaagcg ggttacagcc gccagggtaa cctgtatgcg ggcgataccc
    44041 agaacaccaa ctctgacgct tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt
    44101 atcgccagaa ctactcgctg acctggaacg gtggctggga taacggcgtg accaccagca
    44161 actgggtgca gtacgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta
    44221 ccgaagggaa atttaacgaa aaagcgacac aggatttcgt cgatatcgat cttgatgacg
    44281 tgatgctgca cagcgaagtt aacctgccga ttgatttcct cgttaaccag acgctgacgc
    44341 tgggtacgga gtggaatcag caacggatga aggacttgag ttccaatacc caggcgctga
    44401 ccggggcgaa caccggcggc gctattgatg gtgtgagtgc caccgaccgt agcccgtatt
    44461 caaaagcaga aattttctcg ctgtttgccg aaaacaacat ggagctgact gacagcacca
    44521 tcgtaacgcc ggggctgcgt ttcgatcatc acagtattgt cggcaataac tggagcccgg
    44581 cgctgaacat atcgcaaggt ttaggcgatg acttcacgct gaaaatgggc attgcccgtg
    44641 cttataaagc accgagcctg taccagacta acccgaacta cattctctac agtaaaggtc
    44701 agggttgcta tgccagcgcg ggcggctgct atctgcaagg taacgatgac ctgaaagcag
    44761 aaaccagcat caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggcg
    44821 tcacctggtt ccgtaacgat tatcgcaata agattgaagc gggctatgtg gctgtaggac
    44881 aaaacgcagt cggcaccgat ctctatcagt gggataacgt gccgaaagca gtggttgaag
    44941 gtctggaagg atcgttaaac gtaccggtta gcgaaacggt gatgtggacc aataacatca
    45001 cttatatgct gaagagtgaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt
    45061 atacgttgaa ctcaacgctg agctggcagg cacgagaaga tttgtcgatg caaacgacct
    45121 tcacctggta cggtaagcag cagccgaaga agtacaacta taaaggtcag ccagcggttg
    45181 gcccggaaac caaagaaatc agtccttaca gcattgtggg tctgagcgcg acctgggatg
    45241 tgacgaagaa tgtcagtctg accggcggcg tggacaacct gttcgacaaa cgtttgtggc
    45301 gtgcgggtaa tgcccagacc acgggcgatc tggcaggggc caactatatc gcgggtgccg
    45361 gggcgtatac ctataacgag ccgggacgta cgtggtacat gagcgtaaac actcacttct
    45421 gatgctaacg tcagattgtt gacaaagtgc gcgtcgttca tgccggatgc ggcgtgaacg
    45481 ccttatccgg cctacaaaaa tcttgccaat tcaatatatt gcaggaccat gtaggcctga
    45541 taagcgtagc gcatcaggca gtttagcgtt tatcatcagt ctcggatatg gtcgatatga
    45601 aaactacgca tacctccctc ccctttgccg gacatacgct gcattttgtt gagttcgatc
    45661 cggcgaattt ttgtgagcag gatttactct ggctgccgca ctacgcacaa ttgcaacacg
    45721 ctggacgtaa acgtaaaaca gagcatttag ccggacggat cgctgctgtt tatgccttac
    45781 gggaatatgg ctataaatgt gtgcccgcaa tcggcgagct acgccaacct gtctggcctg
    45841 cggaggtata cggcagtatt agccactgtg ggactacggc attagccgtg gtatctcgtc
    45901 aaccgattgg cattgatata gaagaaattt tttctgtaca aaccgcaaga gaattgacag
    45961 acaacattat tacaccagca gaacacgagc gactcgcgga ctgtggttta gccttttctc
    46021 tggcgctgac actggcattt tccgccaaag agagcgcatt taaggcatcg gagagacaaa
    46081 ctgaagcagg ttttctggat tatcagataa ttagctggaa taaacagcag gtcatcattc
    46141 atcgtgagaa tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataactc
    46201 tgtgccaaca cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgaccg
    46261 cgggcgaaag tgtaaagcag gtgccttacc actctgacct gacaaccgga tatgcgggga
    46321 ttgctccccg cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat
    46381 attacgttcc tttacggtga actcacacag cgagtctccg accagaagcg taaatcccag
    46441 taccgtcaaa cacagcacta tgactgccac aagggcatat ttcgtcagca tgtttatatt
    46501 gcctccatgc gaagaaggga ctaccatccg agttgttgag gctcggagtg gcagccccag
    46561 gattgataga aatatctcct ggggcttcat ctttccagac ctcagagtta acctgaaacc
    46621 ggaaagcttc aggcacccgc cgttatctta cccttcctca acaaaatagc tgtttattta
    46681 tacagtgaat cctgtttcgg acttctgtcg gcaaaactca tcattgctct taaaacgaaa
    46741 tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag caggtgcctt accatcctga
    46801 cctgacaacc ggatatgcgg ggattgctcc ccgcacaacg gctacttctt cggttcgtaa
    46861 gcgagaacag ccttaaactc aatattacgt tcctttacgg tgaactcaca cagcgaatcc
    46921 ccgaccagaa gcgtaaatcc cagcaccgtt aaacacagca ctatgactgc cgcaagggca
    46981 tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat ccaaattgtt
    47041 caggtttggg taggtagccc cagggttgat agaaatatcg tcctggggct tcatctttcc
    47101 agacctcaga gtaaacctga aaccggaaag cttcaggcac ccgcagttat cttacccttg
    47161 cttagcaaaa tagctgttta tttatacagc aagtcctgtt tcggacttct cactccccat
    47221 ttgatcaact gaagctgaac ccgttttttt ctgcatgcct caatcgtcaa aattcttcgc
    47281 caccagccag ccgtgccata acttgtcaca ttgcaaaagt agccaaattg cttcagtcac
    47341 catcaccaaa aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct
    47401 atactgaata gaaaacgcca acttaagaga aacctatgcc attacccgat tttcatgttt
    47461 ctgaaccttt taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg
    47521 acttaagcca ggactcttca atgctgattg acgcggttaa aaataagatc acggccggag
    47581 aggtaaagca cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata
    47641 tcaaccaggc tgccgggcaa ttttcagcga tgcagaaagt cgtattgcag gcagccgcag
    47701 accatcatct ggaaatttgc ggcggtggca cacacccgtt tcagaaatgg cagcgacagg
    47761 aggtatgcga taacgagcgc tatcaacgaa cgctggaaaa ctttggttat ctcattcaac
    47821 aggcgaccgt ttttggtcag catgtccatg ttggctgcgc cagtggcgat gacgccattt
    47881 atttgctgca cggcctgtca cggtttgtgc cgcacttcat cgccctttct gccgcgtcgc
    47941 cgtatatgca gggaacggat acgcgtttta cctcctcacg accgaatatt ttttccgcct
    48001 ttcctgataa tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc
    48061 gctgtctgag ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc
    48121 ccagtcctca ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc
    48181 acgcggtaaa tatggcggga ttaattcagg ctaccgccca ctggttactg acagaacgcc
    48241 cgttcaaaca tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgtc
    48301 gctatgggct ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg
    48361 aagacacctt gcgattgctg gaaaaaatcg ccccttccgc acataaaatt ggtgcatcga
    48421 gcgcgattga ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc
    48481 gcgatttcgt cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct
    48541 gggccggtga ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta
    48601 acttacattt aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat
    48661 tttgctgatg gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct
    48721 ggcacaaaaa ctggtgacca tgttccatct gatggatctt agccagcttt acactttatt
    48781 gttttgtctg tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg
    48841 gcgacgctgg ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact
    48901 ttgcttattc tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg
    48961 cgaaacgcct ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt
    49021 tcgatgtttt taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc
    49081 cctttatcaa tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga
    49141 gcattaagcc agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg
    49201 aaatctccga agcgttactt cgcgatctga tcaacgattc gtggaatctg gtggttgatg
    49261 gtctggctaa acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca
    49321 tttttctcgc ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt
    49381 cacatggagt ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat
    49441 ttgatgccag taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat
    49501 acagcccatc cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag
    49561 gtaaagcgcg tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgcaaaatac
    49621 ttgatgcctc gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga
    49681 agctggttgt tgaccaggaa gatgctgatg gccgctttgc cacgccggaa gcgaaagccg
    49741 cgaacgataa aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg
    49801 cagagtggat ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg
    49861 cgctgggtct ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat
    49921 ttggtctgaa agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg
    49981 ttgaagattt taacgctacg ctgccgaaat cacgtctgcc gcaaaacatc accttaaccg
    50041 aagtgtaatt ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt
    50101 tgcataaccc tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca
    50161 ggatttaata tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat
    50221 ggtgatgatt ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg
    50281 ggtggtactg cggaccttcg aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat
    50341 cattacccag aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa
    50401 tattcaggcg gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg
    50461 cgcgcagttg tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgtgat
    50521 tttgaatctg gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca
    50581 ggggattaaa ctgatcggcg cgattctggt cggcattttg atgatctcgc tgctgattgg
    50641 tcagtcgcca gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt
    50701 ttttaaagat ccgattcttg gtctggtggc aggtattcag ctttccgcga acgatatgct
    50761 gaaactgggc gactggctgg agatgccgaa atacggcgcg gatggcgcgg tgatcgatat
    50821 tgggttaacc accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg
    50881 gtctctggtt tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg
    50941 tattaagcgc agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat
    51001 gcaacgtctg aataaagcgc atttgttaaa gccttattta accagccgcc atcaggaaat
    51061 taatgagtgg aatcgccagc aaggttctac ggagtcgata ttaaacctgc gccgaatgac
    51121 caatattgga acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg
    51181 taaagatatg actttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga
    51241 gatctatgcg tttaccaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat
    51301 attcgatcac atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac
    51361 cggcaatgat attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc
    51421 atcgaatgga tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga
    51481 cgcagcgttt taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac
    51541 aagcgcatat catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcaa
    51601 agatagttgc cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc
    51661 atcgctgcac gaaaacgcag atgcgccagg cagatcataa tccagttcaa cagcagcgtt
    51721 gctaccacca gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaat
    51781 accaccagcg aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg
    51841 ctgacgcgag tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg
    51901 ctgttagagt aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc
    51961 agcgcagaag ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg
    52021 ttggatttca cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa
    52081 aacagcagga tgcgatacac cacctgattt accgcttttg gaatgctttt ttccggatcg
    52141 cgcgcttcag cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc
    52201 gccagcgaca aaatcagccc attccagccg gtggcaaaga aaccaccgta gcgccagagg
    52261 ttgtcgatac tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg
    52321 atcataccga tgatcgccag cactttaatc agcgcaaacc agaactcggt ttcaccatat
    52381 aagcgcacgt tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc
    52441 cacgttggaa catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc
    52501 attcccacca gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt
    52561 ccccagtatt tataggcaaa gtgggcaaat gaaccggata ccggctcttc gaccaccatc
    52621 tcgccaagct ggcgcataat caggaaagcg atgatcccgg cgacgccgta gcccagcaat
    52681 acagccggac ccgccatctg aatcgccggg ccaatgccaa gaaacagacc agtaccaatt
    52741 gcgccaccca gcgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc
    52801 tcttgattcg acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt
    52861 gtgtcttttt tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat
    52921 ccacacggtt aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca
    52981 tcagcttata cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc
    53041 caatcatcgg tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa
    53101 tcggcagcag accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagga
    53161 ccgcgccgtg atataacgcc tcatccagct tctgctcgct gaatgtttgt ggattattca
    53221 acgacggctc ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg
    53281 cggcgacccc ggcgagggcg ataaagccag tgcccgtcgc cacggaaaga tgaaagccca
    53341 tccaccacag caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca
    53401 acgcttcgcc cacccgacgg aacgccagat acaacagcac gaagataatc atcaacgtca
    53461 tcggcaccat gagtttaagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga
    53521 atgccacgct ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt
    53581 gaaccaccga caccatgtca cgatcgcggg catcgatata aatccagctc gtcgggcgcg
    53641 cattctcggt tttcagcatt gacggtccgg tagagacttt aatgtcggcc acgtctgcca
    53701 gggtgatttg ctgcttcatc ggcgtcagaa tcggcagctg gcgcagtgcc tgcggactat
    53761 cgcgccagct ttgcggataa cgcagattaa ttggataacg ggcaattcct tccaccgttt
    53821 cgccaaccat cgccccgccc accgtagaag tcacaaacaa ctgcacatcc gccaccgtca
    53881 taccgtaacg cgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca
    53941 gacgctcggc aagcgcagaa gccacgcctg gtaccgttcg tgccacctct tcaatttgct
    54001 ccgccatcgc gtcgatatcc gccagcacag tgccggaaac tttaattccg atggggcttt
    54061 taatgccagt cgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca
    54121 gccccggcag acgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgcctg
    54181 gccgccactg ctcctgcggc ttaagctgga tggtcgtttc taccatctcc agcggagcag
    54241 aatcggtggc ggtttccgct ttcccggttt tcccaaatac ccgcgccact tcaggtacgc
    54301 tcataattag cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg
    54361 gcagcgtcga tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc
    54421 caactttatt gagcggccag agaaccgtca gcaccgaaag cgccgccacc agcagcgtgg
    54481 ttttcggcca gtgcagtact ttcagcaaca gcggatgata aacacgaatc aaaaagcgat
    54541 tgagcgggtt actgctttcc ggcggaattt tgccacggat ccagtagccc atcaggatcg
    54601 ggatcactac gatcgccagc agcgccgcac ccgccatcgc atacgttttg gtgaacgcca
    54661 acgggccaaa cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg
    54721 tgataatcag cagactgata aatagcgccg gacccacttc aacagatgca tcggtgatca
    54781 cctgccagcg cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca
    54841 gccgtttatg tgcattttcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa
    54901 tcgcaatgcc gcccaacgac ataatattgg cattcagtcc ctggaagtgc atgacaataa
    54961 aagcaataca caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc
    55021 agagaaacag cgcacagact accgccacca caataaactc ttccagcaac ttgccgctga
    55081 ggttgtcgat ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc
    55141 cttccggcag actacttttc agcgtttcca gtttgtcctt cacggcggcg atcacttctc
    55201 gggcgttttt gccggatcgc aggatcacca ccccgcccgc cacttcacct tcgccattca
    55261 gttcggcaat gccccggcgc atctccgggc caatctggac cttcgcaaca tcacgcagat
    55321 aaacgggcac gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt
    55381 gcagatagcc gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc
    55441 ccgcttcctg gtttgaagca tccagcgcgc tttttacttc ggctagactg atgccatatt
    55501 gcgccaggcg ctggggatcg ataaccacct gatactcttt caccacgccg cccaccgacg
    55561 ccacttccgc aacgtcaggg atggttttta gctcatattt gagaaaccag tcctgcaatg
    55621 agcgtaaatc ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc
    55681 agccaacacc cgtggcatct ggtcccagct cggcgctgac tcccgcaggc agcttaccct
    55741 gtacctggtt gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtaccatctt
    55801 cgaaaatgac atacacataa gagtcgccaa actgtgagaa accgcgcaca gtcttcgcgc
    55861 caggcaccga caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgcg
    55921 gtgcctgacc gggatagctg gttttaataa tcacctgcac atcggagaga tccggcagcg
    55981 catccactgg cgtgttaatg atggtccagg tgccccagat gctcagaaac aacgcgccca
    56041 tcagcaccaa aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt
    56101 ccctcaatgc gcattggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt
    56161 ggcttcggaa tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc
    56221 agagtgtaac gcggtgacgc cttgcgatgc ctggaaaacg gcaacgcgtt tcggtacaaa
    56281 gcgtccgtcg gcatcaacgg taatcacccg ctgttcgttg ccggtatcaa tcagcgcctg
    56341 tgacggaatg agcagcatcg gttcgctggc ggtattgagt tgcaaccagg cgttcattcc
    56401 cggttttagc gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc
    56461 ggcatccacg ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgcgc
    56521 cggaacggtg agggtaaact gcgaggcatc tttcaccagc caggcgatgg actccgggat
    56581 cgcagcagtg acccacaccg ggtccatacc ctgaattttc gcgaccacgt tatctttggc
    56641 gatattcatt cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gcgctttgag
    56701 cgtaaagcga gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat
    56761 tcccgccagt cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc
    56821 gcgcagcagt aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag
    56881 cggtgtgccc ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc
    56941 ggcagcgcga gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa
    57001 actctgggca aaagtcagcg gcccgcgcgt gacggtagcc gttttcaccc ccagattctg
    57061 cgtctgagtc gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg
    57121 caccagatcc atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta
    57181 catcgggtcg taccagaata agattttacg ttctgcggtc gacgtttttt ctgcgggtgg
    57241 ttcagccttt gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct
    57301 gccgataata agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat
    57361 atcctgtaat aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccagt
    57421 tttaatttca ctcattttcg tctgcggagt gatggtaaag cgcatggtca tctccggcca
    57481 gttcacggca gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaatacc
    57541 ctttaccacg ccagtggcgc taataacctg cggttgtact tcgctcatgg tttcatgatg
    57601 atgttcgtta gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg
    57661 cagtgctttt ttcatttatt ctctcctgga gttaaaagtc atactctaaa taattcgagt
    57721 tgcaggaagg cgacaagcga gtgaatcccc aggagcttac ataagtaagt gactggggtg
    57781 agcgaacgca gacgcagcac atgcaacttg aagtatgacg agtatattgc tgtcaaccgc
    57841 cacctaaggc ggtatacaaa gaaatttcgt taacctgacg ggcataattc aggtcgagta
    57901 aagtttgtcg ggttgcaaat aaagaacgct cggcatccag cacttccaga taacttaccg
    57961 cgccgtgctg atataatgcc cgcgcccgtt gcaaagtaat ttgcagcgac gccagataac
    58021 gctgctgggc gctgatttga tcgttcaggc tttgacgtaa tgccagcgca tctgccactt
    58081 ctttaaaggc gttctggatt ttctgttcat aattcactac cgactgctgc tggcgaattt
    58141 cggcgatatc cagattggct tggttgcgtc cggcattaaa aatggggatc tcaattttag
    58201 gaataaaatt ccacatcccg ctgctagcgt taaataatga agacagatcg ctgctggcgg
    58261 ttgatattcc gctggtcagg ctgatagacg ggaaaaatgc cgcacgcgca gcgccaatat
    58321 tggcattagc cgccattaac gcgtgttcag cttccataat atccgggcgc tgcaataaga
    58381 tttgcgacga caatcccgcc ggtaatttaa cgctttgcag gctgtcgctg tttactgtct
    58441 gcgcttgcgg cagcttgccg tagcttccca ataacagttg caatgcatta ttcgcctgcg
    58501 ccagttcccc ctgacgttta gcgatgtcgc tgcgggtact ttctatcacc ccgcgagcct
    58561 gttccagcgc cagaacatta ctgctacctg tcaacagttg tttttcgaca aacgcatatg
    58621 actgctgata attacgcagc gtttcttcgg ctatttgcaa ttgcgcatac gccagttgct
    58681 gattgaaata gctttgcgcg acattagaaa ccagcagaat atgcaccgcg cgctgagctt
    58741 cctcagtggc taaataattt tgtcgctcgg cttcgctcat gttctttaag cgaccgaaaa
    58801 aatcgaggtc aaagctggcg ttaaggccgg tcgaaaactc ccgtgtcgtg gcggtattcc
    58861 ctttaagatt accgctccag ctgccgctgc cctcgccatt gagctgtggg tagcggtcgg
    58921 catcggtcag acgatattgc gcccgtgcct cctgcacttt cagcgtcgcc atgcgcaaat
    58981 cccgattatt caccagcgcc tcgctaatca gcgtcttcac ctgattatca acaaaaaagg
    59041 tgcgccagcc cgcgttctga tagttatctg ctgcgttaac caggccgttc tggctgagtg
    59101 agaactgctg cggcacgggc attgccggac gctgataatc cggtgccagt gaacaaccgg
    59161 ttagcgcaag ggccacacaa aatggcagaa gtttacaagg agacataggc tcataatttc
    59221 tggtgatttt atgccgccaa ctttactcgc caggctctga ttttccggtg acagaaaaat
    59281 gacaaaattg tcattttgcc aataagcgat tgccatctga tcccgctact ctagaattgc
    59341 ccgggcaaca tgcggaggaa atatgaaact gttgattgtc gaagatgaaa agaaaaccgg
    59401 agaatacttg accaaagggt taaccgaagc cggttttgtg gtcgatttgg ccgacaacgg
    59461 gttgaatggc taccatctgg cgatgaccgg tgattatgat ctgataatcc tcgatattat
    59521 gctgccggac gtgaacggct gggatatcgt gcgcatgttg cgctccgcca ataaagggat
    59581 gccgattctg ttgcttaccg cgcttggcac cattgaacat cgcgtcaagg ggctggagtt
    59641 gggggccgat gactatctgg tgaagccgtt cgcttttgct gaactgctgg cgcgggtgcg
    59701 cactctcctg cggcgcgggg cggcggtgat tatcgaaagt cagtttcagg ttgccgatct
    59761 gatggtcgat ctcgtcagcc gcaaagtcac ccgcagcggc acgcgcatca ctctgaccag
    59821 taaagagttt actctgctgg agttttttct ccgccatcag ggcgaagtgc tgccccgctc
    59881 gcttatcgcc tcgcaggtgt gggacatgaa tttcgacagc gacactaacg ccattgatgt
    59941 ggcggtgaag cggctacgcg gcaaaatcga caacgacttt gagccgaagc tgattcagac
    60001 cgtgcgcggc gtgggctata tgcttgaggt gccggatggt cagtaagcca tttcagcgcc
    60061 cgttttcgct ggcaacccgt ctgacctttt ttatcagcct ggccaccatc gcggcgtttt
    60121 tcgcctttgc atggatcatg atccactcag tgaaagtgca ttttgccgag caggatatta
    60181 atgatttaaa agaaattagc gccacccttg aacgggtact taatcatcct gacgagacgc
    60241 aagcccgacg cttaatgacg ctggaagata tcgtcagtgg ttattccaac gtgttgattt
    60301 ccctggcaga tagccacggc aaaacggtgt atcactcccc cggtgcgccg gatatccgcg
    60361 agtttacgcg tgacgccata cccgataaag acgctcaggg tggcgaggtg tatctccttt
    60421 ccggcccgac gattatgatg ccaggccacg gtcacgggca tatggaacac agcaactggc
    60481 ggatgattaa cttgccggtt ggcccgttgg tggacggcaa accgatttat acgctctaca
    60541 tcgcgctttc gatcgatttt catcttcatt acataaatga tttgatgaat aaacttatta
    60601 tgaccgcatc tgtcatcagc atcctgatcg tctttatcgt gctattggcg gtacataaag
    60661 gtcacgcgcc gatccgcagc gtcagccgtc aaatccagaa tatcacctcg aaagatctcg
    60721 acgttcgcct tgacccgcag accgtgccca ttgagctgga acagctggta ctgtcgttca
    60781 accatatgat cgagcgtatt gaggatgtct ttacccgcca gtccaatttc tcagcggata
    60841 tcgctcacga aattcgcacg ccgattacca atctcatcac gcaaacggaa atcgccctca
    60901 gccagtcgcg cagccagaag gaactggaag atgtgctcta ctctaatctc gaagagctga
    60961 cgcgaatggc gaaaatggtc agcgatatgc tgtttctcgc tcaggccgat aacaaccagc
    61021 taatccccga aaagaaaatg ctcaacctgg cggatgaagt cggcaaagtg ttcgattttt
    61081 tcgaggcgtt agcggaagat cgcggcgtgg agttgcggtt tgttggcgac aaatgtcagg
    61141 ttgcgggcga tccgctgatg ctgcgtcggg cgttaagcaa cctgctttct aatgccctgc
    61201 gttatacgcc acccagtgag gctattgtag tgcgctgcca gacggtcaat catcaggtgc
    61261 aagtttccgt cgaaaacccc ggtacgccca ttgcgcccga gcacttaccg cgattgtttg
    61321 accgtttcta tcgcgttgac ccttcccgcc agcgaaaagg tgaaggtagc ggtattgggc
    61381 tggcgatagt gaaatcgatt gttgtcgcgc ataaaggcac ggttgcggta acgtcagatg
    61441 cgcgggggac aaggtttgtg attatgctgc cggagagaga gtgattatta agttcaaatt
    61501 cagggcgtgg tgcatcacct cctggtattg ttgatcaaca aattagagaa aaacttatta
    61561 aacatatgaa atatcgaagg cttttatttt tcaaagtttc acgagatgtt tgtatatgaa
    61621 acttagttaa atctgtgttt cttccaattg tagatcacaa tgccctttct aaattttcat
    61681 cgttataaac aagccatcta tcatataaag caatgaataa tttaatcgcg attaatcatc
    61741 attaattgtt atattattca ctcaatctat ttatcaagtg aatttgataa attcctaatt
    61801 taaaatatcc actattgagc cagt
//
LOCUS       DAZGXZ010000011        60517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000011 DAZGXZ010000000
VERSION     DAZGXZ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.011"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(158..1633)
                     /gene="ampG"
                     /locus_tag="ACHKJF_000814"
     CDS             complement(158..1633)
                     /gene="ampG"
                     /locus_tag="ACHKJF_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP0948224.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1677..2255)
                     /gene="yajG"
                     /locus_tag="ACHKJF_000815"
     CDS             complement(1677..2255)
                     /gene="yajG"
                     /locus_tag="ACHKJF_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0948225.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2560..2877
                     /gene="bolA"
                     /locus_tag="ACHKJF_000816"
     CDS             2560..2877
                     /gene="bolA"
                     /locus_tag="ACHKJF_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP0948226.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3221..4519
                     /gene="tig"
                     /locus_tag="ACHKJF_000817"
     CDS             3221..4519
                     /gene="tig"
                     /locus_tag="ACHKJF_000817"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0948227.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4765..5388
                     /gene="clpP"
                     /locus_tag="ACHKJF_000818"
     CDS             4765..5388
                     /gene="clpP"
                     /locus_tag="ACHKJF_000818"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP0948228.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5514..6788
                     /gene="clpX"
                     /locus_tag="ACHKJF_000819"
     CDS             5514..6788
                     /gene="clpX"
                     /locus_tag="ACHKJF_000819"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP0948229.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6976..9330
                     /gene="lon"
                     /locus_tag="ACHKJF_000820"
     CDS             6976..9330
                     /gene="lon"
                     /locus_tag="ACHKJF_000820"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP0948230.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9521..9811
                     /gene="hupB"
                     /locus_tag="ACHKJF_000821"
     CDS             9521..9811
                     /gene="hupB"
                     /locus_tag="ACHKJF_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP0948231.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            10003..11874
                     /gene="ppiD"
                     /locus_tag="ACHKJF_000822"
     CDS             10003..11874
                     /gene="ppiD"
                     /locus_tag="ACHKJF_000822"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0948232.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            12025..12396
                     /gene="ybaV"
                     /locus_tag="ACHKJF_000823"
     CDS             12025..12396
                     /gene="ybaV"
                     /locus_tag="ACHKJF_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP0948233.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKMVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12490..12888
                     /gene="fadM"
                     /locus_tag="ACHKJF_000824"
     CDS             12490..12888
                     /gene="fadM"
                     /locus_tag="ACHKJF_000824"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP0948234.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12940..13635)
                     /gene="queC"
                     /locus_tag="ACHKJF_000825"
     CDS             complement(12940..13635)
                     /gene="queC"
                     /locus_tag="ACHKJF_000825"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP0948235.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13700..15400)
                     /gene="ybaE"
                     /locus_tag="ACHKJF_000826"
     CDS             complement(13700..15400)
                     /gene="ybaE"
                     /locus_tag="ACHKJF_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP0948236.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15500..16318
                     /gene="cof"
                     /locus_tag="ACHKJF_000827"
     CDS             15500..16318
                     /gene="cof"
                     /locus_tag="ACHKJF_000827"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP0948237.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16470..16928
                     /gene="decR"
                     /locus_tag="ACHKJF_000828"
     CDS             16470..16928
                     /gene="decR"
                     /locus_tag="ACHKJF_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP0948238.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16958..18730
                     /gene="mdlA"
                     /locus_tag="ACHKJF_000829"
     CDS             16958..18730
                     /gene="mdlA"
                     /locus_tag="ACHKJF_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0948239.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALMEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18723..20504
                     /gene="mdlB"
                     /locus_tag="ACHKJF_000830"
     CDS             18723..20504
                     /gene="mdlB"
                     /locus_tag="ACHKJF_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0948240.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVVKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20685..21023
                     /gene="glnK"
                     /locus_tag="ACHKJF_000831"
     CDS             20685..21023
                     /gene="glnK"
                     /locus_tag="ACHKJF_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP0948241.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            21053..22339
                     /gene="amtB"
                     /locus_tag="ACHKJF_000832"
     CDS             21053..22339
                     /gene="amtB"
                     /locus_tag="ACHKJF_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP0948242.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22388..23248)
                     /gene="tesB"
                     /locus_tag="ACHKJF_000833"
     CDS             complement(22388..23248)
                     /gene="tesB"
                     /locus_tag="ACHKJF_000833"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP0948243.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23466..24038
                     /gene="ybaY"
                     /locus_tag="ACHKJF_000834"
     CDS             23466..24038
                     /gene="ybaY"
                     /locus_tag="ACHKJF_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP0948244.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24069..24380)
                     /locus_tag="ACHKJF_000835"
     CDS             complement(24069..24380)
                     /locus_tag="ACHKJF_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP0948245.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24543..24639
                     /gene="ffs"
                     /locus_tag="ACHKJF_000836"
     ncRNA           24543..24639
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKJF_000836"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24759..25112
                     /gene="ybaA"
                     /locus_tag="ACHKJF_000837"
     CDS             24759..25112
                     /gene="ybaA"
                     /locus_tag="ACHKJF_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP0948246.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25154..26704)
                     /gene="pdeB"
                     /locus_tag="ACHKJF_000838"
     CDS             complement(25154..26704)
                     /gene="pdeB"
                     /locus_tag="ACHKJF_000838"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP0948247.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26868..27338)
                     /gene="ylaC"
                     /locus_tag="ACHKJF_000839"
     CDS             complement(26868..27338)
                     /gene="ylaC"
                     /locus_tag="ACHKJF_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP0948248.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27454..28005)
                     /gene="maa"
                     /locus_tag="ACHKJF_000840"
     CDS             complement(27454..28005)
                     /gene="maa"
                     /locus_tag="ACHKJF_000840"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP0948249.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSQAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(28176..28394)
                     /gene="hha"
                     /locus_tag="ACHKJF_000841"
     CDS             complement(28176..28394)
                     /gene="hha"
                     /locus_tag="ACHKJF_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP0948250.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(28420..28794)
                     /gene="tomB"
                     /locus_tag="ACHKJF_000842"
     CDS             complement(28420..28794)
                     /gene="tomB"
                     /locus_tag="ACHKJF_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP0948251.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(29340..32489)
                     /gene="acrB"
                     /locus_tag="ACHKJF_000843"
     CDS             complement(29340..32489)
                     /gene="acrB"
                     /locus_tag="ACHKJF_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP0948252.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(32512..33705)
                     /gene="acrA"
                     /locus_tag="ACHKJF_000844"
     CDS             complement(32512..33705)
                     /gene="acrA"
                     /locus_tag="ACHKJF_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP0948253.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            33847..34494
                     /gene="acrR"
                     /locus_tag="ACHKJF_000845"
     CDS             33847..34494
                     /gene="acrR"
                     /locus_tag="ACHKJF_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP0948254.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            34622..37984
                     /gene="mscK"
                     /locus_tag="ACHKJF_000846"
     CDS             34622..37984
                     /gene="mscK"
                     /locus_tag="ACHKJF_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP0948255.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            38045..38143
                     /locus_tag="ACHKJF_000847"
                     /pseudo
     CDS             38045..38143
                     /locus_tag="ACHKJF_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(38196..38357)
                     /gene="rsmS"
                     /locus_tag="ACHKJF_000848"
     CDS             complement(38196..38357)
                     /gene="rsmS"
                     /locus_tag="ACHKJF_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP0948256.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(38371..38898)
                     /gene="priC"
                     /locus_tag="ACHKJF_000849"
     CDS             complement(38371..38898)
                     /gene="priC"
                     /locus_tag="ACHKJF_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP0948257.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38968..39345
                     /gene="ybaN"
                     /locus_tag="ACHKJF_000850"
     CDS             38968..39345
                     /gene="ybaN"
                     /locus_tag="ACHKJF_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP0948258.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            39498..40049
                     /gene="apt"
                     /locus_tag="ACHKJF_000851"
     CDS             39498..40049
                     /gene="apt"
                     /locus_tag="ACHKJF_000851"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP0948259.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            40178..42109
                     /gene="dnaX"
                     /locus_tag="ACHKJF_000852"
     CDS             40178..42109
                     /gene="dnaX"
                     /locus_tag="ACHKJF_000852"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP0948260.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    41442..41506
                     /gene="dnaX"
                     /locus_tag="ACHKJF_000852"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            42162..42491
                     /gene="ybaB"
                     /locus_tag="ACHKJF_000853"
     CDS             42162..42491
                     /gene="ybaB"
                     /locus_tag="ACHKJF_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP0948261.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            42491..43096
                     /gene="recR"
                     /locus_tag="ACHKJF_000854"
     CDS             42491..43096
                     /gene="recR"
                     /locus_tag="ACHKJF_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP0948262.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            43206..45080
                     /gene="htpG"
                     /locus_tag="ACHKJF_000855"
     CDS             43206..45080
                     /gene="htpG"
                     /locus_tag="ACHKJF_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP0948263.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            45261..45905
                     /gene="adk"
                     /locus_tag="ACHKJF_000856"
     CDS             45261..45905
                     /gene="adk"
                     /locus_tag="ACHKJF_000856"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP0948264.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            46141..47103
                     /gene="hemH"
                     /locus_tag="ACHKJF_000857"
     CDS             46141..47103
                     /gene="hemH"
                     /locus_tag="ACHKJF_000857"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP0948265.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(47100..48059)
                     /gene="aes"
                     /locus_tag="ACHKJF_000858"
     CDS             complement(47100..48059)
                     /gene="aes"
                     /locus_tag="ACHKJF_000858"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP0948266.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            48211..49515
                     /gene="gsk"
                     /locus_tag="ACHKJF_000859"
     CDS             48211..49515
                     /gene="gsk"
                     /locus_tag="ACHKJF_000859"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP0948267.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(49648..51324)
                     /gene="ybaL"
                     /locus_tag="ACHKJF_000860"
     CDS             complement(49648..51324)
                     /gene="ybaL"
                     /locus_tag="ACHKJF_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP0948268.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLMVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(51562..52782)
                     /gene="fsr"
                     /locus_tag="ACHKJF_000861"
     CDS             complement(51562..52782)
                     /gene="fsr"
                     /locus_tag="ACHKJF_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP0948269.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            53000..54652
                     /gene="ushA"
                     /locus_tag="ACHKJF_000862"
     CDS             53000..54652
                     /gene="ushA"
                     /locus_tag="ACHKJF_000862"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0948270.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFASVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(54689..55168)
                     /gene="ybaK"
                     /locus_tag="ACHKJF_000863"
     CDS             complement(54689..55168)
                     /gene="ybaK"
                     /locus_tag="ACHKJF_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP0948271.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVDVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(55372..56166)
                     /gene="ybaP"
                     /locus_tag="ACHKJF_000864"
     CDS             complement(55372..56166)
                     /gene="ybaP"
                     /locus_tag="ACHKJF_000864"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP0948272.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            56304..56645
                     /gene="ybaQ"
                     /locus_tag="ACHKJF_000865"
     CDS             56304..56645
                     /gene="ybaQ"
                     /locus_tag="ACHKJF_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0948273.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(56759..59263)
                     /gene="copA"
                     /locus_tag="ACHKJF_000866"
     CDS             complement(56759..59263)
                     /gene="copA"
                     /locus_tag="ACHKJF_000866"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP0948274.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSCHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            59525..60457
                     /gene="glsA"
                     /locus_tag="ACHKJF_000867"
     CDS             59525..60457
                     /gene="glsA"
                     /locus_tag="ACHKJF_000867"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP0948275.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT        15009 a        15315 c        16068 g        14125 t
ORIGIN      
        1 agctgaatcg tgtaagataa atagaattca taaatagcgt caataatttc caaacgctaa
       61 gccgcacaaa agaacaaata ttaataagcg gtgctgatgt ttatttagcc gtaataatta
      121 cggcgggtga ttactacagc taaataatat ttacagatta cgtcagatgc gtttttcgta
      181 gcgccagata atccagcaaa ccaccaagca cgacgccaga aagggcgact aaaaccccga
      241 cttccagcag agcaggcagg aaagagaagt gcgtcaaatc cagcgcgtcc atcgtcaaca
      301 gcagtaacca cacggccaac aggctgacgc ccgccgccag tgtccacatg gcaaaggcat
      361 aacctgccgg atattcggta cgggagataa agttgtcatt tactcgtgta tattcaagcg
      421 tctggcggca aaccagcagc aaaataagcc ccggtacggc agcggcgacg gagaatagat
      481 agaatgtcga ccagccgtgt gcttcaacaa accaacccgc cacggggccg acataaactc
      541 gccctacagc ggaaagcgct gagagcaggg caaattgagt tgcggaaaat gacttattac
      601 acagcgtcat taacagcgcg acaaaggctg atgtgcccat cccgccacag aggttttcga
      661 aaaagacggc tgcgcccatg ctgtagagat gcttatcagt aatcgacagc agccagtaac
      721 cagcgttaga cgcaccttgt aaaatgccga aaatcagcag cgcccggaac agcgacaggc
      781 gctgcatcaa aatcccaccg tacaatgcgc ccacaatggt cgccagtaag ccaagcgttt
      841 tgttaaccac gcctacttca cccgcatcaa acccgacgcc gcgaatcaaa aacgtggttg
      901 tcaggctcat ggcgaatgcg tcgcccagct tatacagcac gataagaagc aaaataagcc
      961 aggcattatt gcgaccaaag aaatctcgca gaggtgcaac aaccgcttgt tccagcgttt
     1021 ttggcacagg aatggtgtcg gtcggttctg gtgcaagcaa cgtcgcgata atacaaggga
     1081 tcaacagcgc cgccatcaac cagtacatgc cctgccagcc cagccattta tctgccagcc
     1141 acagggctaa gccgccggaa accagcatcc ctaaacggta acccagcacg ctgattgccg
     1201 cgcccgcacc acgttcttct gccgggagca catcggtttt ccacgcatcg aagacaatat
     1261 cctgggaggc cgagcaaaaa gcgatcacca ccgccagtgc cgccatccag cggagttggg
     1321 tgccaggttc gagaaaaccc atcgccgcaa tggcgactaa taacaggatt tgcgtggcga
     1381 gcagccaacc gcgccgccgc ccaaaaaatg ggggcgtgta gcggtccatc agcggtgacc
     1441 agaggaattt aaaaacgtaa gcctggccta ccagagagaa gaaaccaatg gttttgagat
     1501 caatattctc gaccgtcatc caggcctgta aggtgccgga tgtcagggcg agcggtagcc
     1561 cggaagcaaa acccaggatc agcaatatgg ctgaacgcgg ctgttgaaaa atacgtaaat
     1621 attgactgga catgggcgtc tacaggcccg gcttgcaccg ggccagaggg cagaaattaa
     1681 cgcgcgttct gcttgatgaa ttcgtgaatg ctggtgtcct gagacatatc agcgatggta
     1741 tcggtcagca cactgttaac cgcatcggcg atatttttgt tggaggcctg gaacgcacct
     1801 tcaacgttgt agctggcacg atagtttttg gtcattttgt tgccattctg cgcggtagcg
     1861 atgatggcga tatccgcttt ggtagcgatg ttgtagcgca cgttgccctg ggacacgtca
     1921 gcatacagtt ggctaacgat gatttgcaga ttaaccgggc cattcggacc aaccatgtaa
     1981 ccacgcgcgg tcatctgttt ttccagcact tcttgcagca ggaaacgcag atcgcgggag
     2041 gcggtcaggg taacgatttg attatcgcgg gtgacttttg ccagcgcctg atcggtacgc
     2101 tgatcggcac cattaatgct tacggtgacg cccatcaggc ttggatcctg ctgtggcagt
     2161 gtaatcgtcg gggaaacttc aatagttgtt ggcggttttg cgcatcctgc aagcataaac
     2221 agagcaacta acgggaagag gatttttttg aacatgttcg ggctctcaga gactcttaag
     2281 cgtgtttggt aaaaattccc gccatcataa cattgccaac ggcgagggga agtgggtaag
     2341 gcatgtaaat tcatcatgtt gacgaaataa tcgcccctgg caaaagaaac actgatgcga
     2401 ggcctgtgtt tcaatcttta aatcagtaaa cttcatacgc ttgacggaaa aaccaggacg
     2461 aaacctaaat atttgttgtt aagctgcaat ggaaacggta aaagcggcta gtatttaaag
     2521 ggatggatga catctcagcg ttgtcggagg agatatttca tgatgatacg tgagcggata
     2581 gaagaaaaat taagggcggc gttccaaccc gtattcctcg aagtagtgga tgaaagctat
     2641 cgtcacaatg tcccagccgg ctctgaaagc cattttaaag ttgtgctggt cagcgatcgt
     2701 tttacgggtg aacgttttct gaatcgtcat cgaatgattt acagtacttt agcggaggaa
     2761 ctctctacta ccgttcatgc gctggctctg catacttaca ctattaagga gtgggaaggg
     2821 ttgcaggaca ccgtctttgc ctctcctccc tgtcgtggag caggaagcat cgcgtaaaaa
     2881 cgcatttgca actgtcggcg cttttccagt atgttgctaa agattttatg aaaaacggcc
     2941 tgcgggccgt tttgttttgt ctggattttg cgctttttgc ccagcattca gacgaaaatt
     3001 gcccgggaat tgtgaaaaaa tacgcgacag cgcgcaataa ccgttctcga ctcataaaag
     3061 tgatgccgct ataatgccgc gtcctatttg aatgctttcg ggatgattct ggtaacaggg
     3121 aatgtgattg attataagaa catcccggtt ccgcgaagcc aacaacctgt gcttgcgggg
     3181 taagagttga ccgagcactg tgattttttg aggtaacaag atgcaagttt cagttgaaac
     3241 cactcaaggc cttggccgcc gtgtaacgat tactatcgct gctgacagca tcgagaccgc
     3301 tgttaaaagc gagctggtca acgttgcgaa aaaagtacgt attgacggct tccgcaaagg
     3361 caaagtgcca atgaatatcg ttgctcagcg ttatggcgcg tctgtacgcc aggacgttct
     3421 gggtgacctg atgagccgta acttcattga cgccatcatt aaagaaaaaa tcaatccggc
     3481 tggcgcaccg acttatgttc cgggcgaata caagctgggt gaagacttca cttactctgt
     3541 agagtttgaa gtttatccgg aagttgaact gcagggtctg gaagcgatcg aagttgaaaa
     3601 accgatcgtt gaagtgaccg acgctgacgt tgacggcatg ctggatactc tgcgtaaaca
     3661 gcaggcgacc tggaaagaaa aagacggcgc tgttgaagca gaagaccgcg taaccatcga
     3721 cttcaccggt tctgtagacg gcgaagagtt cgaaggcggt aaagcgtctg atttcgtact
     3781 ggcgatgggc cagggtcgta tgatcccggg ctttgaagac ggtatcaaag gtcacaaagc
     3841 tggcgaagag ttcaccatcg acgtgacctt cccggaagaa taccacgcag aaaacctgaa
     3901 aggtaaagca gcgaaattcg ctatcaacct gaagaaagtt gaagagcgtg aactgccgga
     3961 actgactgca gaattcatca aacgtttcgg cgttgaagat ggttccgtag aaggtctgcg
     4021 cgctgaagtg cgtaaaaaca tggagcgcga gctgaagagc gccatccgta accgcgttaa
     4081 gtctcaggcg atcgaaggtc tggtaaaagc taacgacatc gacgtaccgg ctgcgctgat
     4141 tgacagcgaa atcgacgttc tgcgtcgtca ggctgcacag cgtttcggtg gcaacgaaaa
     4201 acaagctctg gaactgccgc gcgaactgtt cgaagaacag gctaaacgcc gcgtagttgt
     4261 tggcctgctg ctgggcgaag ttatccgcac caacgagctg aaagctgacg aagagcgcgt
     4321 gaaaggcctg atcgaagaga tggcttctgc gtacgaagat ccgaaagaag ttatcgagtt
     4381 ctacagcaaa aacaaagaac tgatggacaa catgcgcaat gttgctctgg aagaacaggc
     4441 tgttgaagct gtactggcga aagcgaaagt gactgaaaaa gaaaccactt tcaacgagct
     4501 gatgaaccag caggcgtaat ttacgcagca taacgcgcta aattcgcaca aaggcccgtc
     4561 accgccaggt ggtgggcttt tttttgtcat gaattttgca tggaaccgtg cgaaaagcct
     4621 ctttcggtgt tagcgtaaca acaaaagatt gttatgcttg aaatatggtg atgccgtacc
     4681 cataacacag ggactagctg ataatccgtc cataaggtta caatcggtac agcaggtttt
     4741 ttcaatttta tccaggagac ggaaatgtca tacagcggcg aacgagataa ctttgcaccc
     4801 catatggcgc tggtgccgat ggtcattgaa cagacctcac gcggtgagcg ctcttttgat
     4861 atctattctc gtctacttaa ggaacgcgtc atttttctga ctggccaggt tgaagaccac
     4921 atggctaacc tgattgtggc gcagatgctg ttcctggaag cggaaaaccc agaaaaagat
     4981 atctatctgt acattaactc cccaggcggg gtgatcactg ccgggatgtc tatctatgac
     5041 accatgcagt ttatcaagcc tgatgtcagc accatctgta tgggccaggc ggcctcgatg
     5101 ggcgctttct tgttgaccgc aggggcaaaa ggtaaacgtt tttgcctgcc gaattcgcgc
     5161 gtgatgattc accaaccgtt gggcggctac cagggccagg cgaccgatat cgaaattcat
     5221 gcccgtgaaa ttctgaaagt taaagggcgc atgaatgaac ttatggcgct tcatacgggt
     5281 caatcattag aacagattga acgtgatacc gagcgcgatc gcttcctttc cgcccctgaa
     5341 gcggtggaat acggtctggt cgattcgatt ctgacccatc gtaattgatg ccagaggcgc
     5401 aactgtgccg ctatacttat ccagggcggc acaacgctgt aagcggcttg cgcctgagaa
     5461 tggcatttgc gtcgtcgtgt gcggcacaaa gaacaaagaa gaggttttga cccatgacag
     5521 ataaacgcaa agatggctca ggcaaattgc tgtattgctc tttttgcggc aaaagccagc
     5581 atgaagtgcg caagctgatt gccggtccat ccgtgtatat ctgcgacgaa tgtgttgatt
     5641 tatgtaacga catcattcgc gaagagatta aagaagttgc accgcatcgt gaacgcagtg
     5701 cgctaccgac gccgcatgaa attcgcaacc acctggacga ttacgttatc ggccaggaac
     5761 aggcgaaaaa agtgctggcg gtcgcggtat acaaccatta caaacgtctg cgcaacggcg
     5821 ataccagcaa tggtgtcgag ttgggcaaaa gtaacattct gctgatcggt ccgaccggtt
     5881 ccggtaaaac gctgctggct gaaacgctgg cgcgcctgct ggacgtcccg ttcaccatgg
     5941 ccgacgcaac cacgctgacc gaagccggtt atgtgggcga agacgttgaa aacatcattc
     6001 agaagctgtt gcagaagtgc gattacgacg tacagaaagc gcagcgcggg attgtctaca
     6061 tcgatgaaat cgacaagatt tctcgtaagt cagacaaccc gtccattacc cgagacgttt
     6121 ccggtgaagg cgtacagcag gcactgttga aactgatcga aggtacggta gctgctgttc
     6181 caccgcaagg tgggcgtaaa catccgcagc aggaattctt gcaggttgat acctctaaga
     6241 tcctgtttat ttgtggcggt gcgtttgccg gtctggataa agtgatttcc caccgtgtag
     6301 aaaccggctc cggcattggt tttggcgcga cggtaaaagc gaagtccgac aaagcaagcg
     6361 aaggcgagct gctggcgcag gttgaaccgg aagatctgat caagtttggt cttatccctg
     6421 agtttattgg tcgtctgccg gttgtcgcaa cgttgaatga actgagcgaa gaagctctga
     6481 ttcagatcct caaagagccg aaaaacgccc tgaccaagca gtatcaggcg ctgtttaatc
     6541 tggaaggcgt ggatctggaa ttccgtgacg aggcgctgga tgctatcgct aagaaagcga
     6601 tggcgcgtaa aaccggtgcc cgtggcctgc gttccatcgt agaagccgca ctgctcgata
     6661 ccatgtacga tctgccgtcc atggaagatg tcgaaaaagt ggttatcgac gagtcggtaa
     6721 ttgatggtca aagcaaaccg ttgctgattt atggtaagcc ggaagcgcaa caggcatctg
     6781 gtgaataatt aaccattccc atacaattag ttaaccaaaa aggggggatt ttatctcccc
     6841 tttaattttt cctctattct cggcgttgaa tgtgggggaa acatccccat atactgacgt
     6901 acatgttaat agatggcgtg aagcacagtc gtgtcatctg attacctggc ggaaattaaa
     6961 ctaagagaga gctctatgaa tcctgagcgt tctgaacgca ttgaaatccc cgtattgccg
     7021 ctgcgcgatg tggtggttta tccgcacatg gtcatcccct tatttgtcgg gcgggaaaaa
     7081 tctatccgtt gtctggaagc ggcgatggac catgataaaa aaattatgct ggtcgcgcag
     7141 aaagaagctt caacggatga gccgggtgta aacgatcttt tcaccgtcgg gaccgtggcc
     7201 tctatattgc agatgctgaa actgcctgac ggcaccgtca aagtgctggt cgaggggtta
     7261 cagcgcgcgc gtatttctgc gctctctgac aatggcgaac acttttctgc gaaggcggag
     7321 tatctggagt cgccgaccat tgatgagcgg gaacaggaag tgctggtgcg tactgcaatc
     7381 agccagttcg aaggctacat caagctgaac aaaaaaatcc caccagaagt gctgacgtcg
     7441 ctgaatagca tcgacgatcc ggcgcgtctg gcggatacca ttgctgcaca tatgccgctg
     7501 aaactggctg acaaacagtc cgttctggag atgtccgacg ttaacgaacg tctggaatat
     7561 ctgatggcaa tgatggaatc ggaaatcgat ctgctgcagg ttgagaaacg cattcgcaac
     7621 cgcgttaaaa agcagatgga gaaatcccag cgtgagtact atctgaacga gcaaatgaaa
     7681 gctattcaga aagaactcgg tgaaatggac gacgcgccgg acgaaaacga agccctgaag
     7741 cgcaaaatcg acgcggcgaa gatgccgaaa gaggcaaaag agaaagcgga agcagagttg
     7801 cagaagctga aaatgatgtc tccgatgtcg gcagaagcga ccgtagtgcg tggttatatc
     7861 gactggatgg tacaggtacc gtggaatgcg cgcagcaagg tcaaaaaaga cctgcgtcag
     7921 gcgcaggaaa tccttgatac cgaccattat ggtctggagc gcgtgaaaga tcgcatcctt
     7981 gagtaccttg cggttcaaag ccgtgtcaac aaaatcaagg gaccgatcct ctgcctggta
     8041 gggccgccgg gggtaggtaa aacctctctt ggtcagtcca ttgccaaagc caccgggcgt
     8101 aaatatgtcc gtatggcgct gggcggcgtg cgtgatgaag cggaaatccg tggtcaccgc
     8161 cgtacttaca tcggttctat gccgggtaaa ctgatccaga aaatggcgaa agtgggcgtg
     8221 aaaaacccgc tgttcctgct cgatgagatc gacaaaatgt cttctgacat gcgaggcgat
     8281 ccggcctctg cactgcttga agtgctggat ccagagcaga acgtagcgtt cagcgaccac
     8341 tacctggaag tggattacga tctcagcgac gtgatgtttg tcgcgacgtc gaactccatg
     8401 aacattccgg caccgctgct ggatcgtatg gaagtgattc gcctctccgg ttataccgaa
     8461 gatgaaaaac tgaacatcgc caaacgtcac ctgctgccga agcagattga acgtaatgca
     8521 ctgaaaaaag gtgagctgac cgtcgacgat agcgccatta tcggcattat tcgttactac
     8581 acccgtgagg cgggcgtgcg tggtctggag cgtgaaatct ccaaactgtg ccgcaaagcg
     8641 gttaagcagt tactgctcga taagtcatta aaacatatcg aaattaacgg cgataacctg
     8701 catgactacc tcggtgttca gcgtttcgac tatggtcgcg ctgataacga aaaccgtgtc
     8761 ggtcaggtaa ccggtctggc gtggacggaa gtgggcggtg acttgctgac cattgaaacc
     8821 gcatgtgttc cgggtaaagg caaactgacc tataccggtt cgctcggcga agtgatgcag
     8881 gagtctattc aggcggcgtt aacggtggtt cgtgcgcgtg cggaaaaact ggggatcaac
     8941 cctgattttt acgaaaaacg tgacatccac gtccacgtac cggaaggtgc gacgccgaaa
     9001 gatggtccga gtgccggtat tgctatgtgc accgcgctgg tttcttgcct gaccggtaac
     9061 ccggttcgtg ccgatgtggc aatgaccggt gagatcactc tgcgtggtca ggtactgccg
     9121 atcggtggtt tgaaagaaaa actcctggca gcgcatcgcg gcgggattaa aacagtgcta
     9181 attccgttcg aaaataaacg cgatctggaa gagattcctg acaacgtaat tgccgatctg
     9241 gacattcatc ctgtgaagcg cattgaggaa gttctgactc tggcgctgca aaatgaaccg
     9301 tctggcatgc aggttgtgac tgcaaaatag tgacctcgcg caaaatgcac taataaaaac
     9361 agggctggca ggctaattcg ggcttgccag cctttttttg tctcgctaag ttagatggcg
     9421 gatcgggctt gcccttatta aggggtgttg taaggggatg gctggcctga tataactgct
     9481 gcgcgttcgt accttgaagg attcaagtgc gatataaatt ataaagagga agagaagagt
     9541 gaataaatct caattgatcg acaagattgc tgcaggggct gatatctcta aagctgcggc
     9601 tggccgtgcg ttagatgcta ttattgcttc cgtaactgaa tctctgaaag aaggggatga
     9661 tgtagcactg gtaggttttg gtacttttgc cgttaaagag cgtgctgccc gtactggccg
     9721 caacccgcag accggtaaag agatcaccat cgctgctgct aaagtaccga gcttccgtgc
     9781 aggtaaagca ctgaaagacg cggtaaacta agcgttgtcc ccggtgggga tgtgacgaag
     9841 ttcaagggcg catctactga tgtgcctttt ttatttgtat tcggtgactt tctgcgtctt
     9901 gtgggctgac aattgccccc gtttcttgtc acaataggcc tttgcgcgca tcgatacgtt
     9961 gcgtgaggta cacagtcatc tacagcggag tgttgttaca ccatgatgga cagcttacgc
    10021 acggctgcaa acagtctcgt gctcaagatt attttcggta tcattatcgt gtcgttcata
    10081 ttgaccggcg tgagtggtta cctgattggc ggaggcaata actacgccgc aaaagtgaat
    10141 gaccaggaaa tcagccgtgg gcagttcgag aacgccttta acagcgagcg taatcgcatg
    10201 cagcaacagc tgggcgatca atactccgag ctggcagcga acgaaggcta tatgaaaacc
    10261 ctgcgtcaac aggtgctgaa tcgtctgatc gacgaggcgc tgttggatca gtacgctcgt
    10321 gagctgaaac tgggtatcag tgatgagcag gttaaacagg cgattttcgc gaccccagcc
    10381 ttccaggttg acggcaaatt tgataacagc cgctataacg gtatcctcaa ccagatgggg
    10441 atgaccgccg atcagtacgc ccaggcgctg cgtaaccagc tcactaccca acagctgatt
    10501 aacggtgttg ccggtaccga ttttatgctg aaaggtgaaa ccgacgagct ggcggcactg
    10561 gtcgcgcaac aacgcgtggt gcgtgaggcg actatcgatg ttaacgcgct ggcggcgaag
    10621 cagcctgtga ccgaacagga gattgccagc tactacgaac aaaacaaaaa caatttcatg
    10681 acgccggaac aattccgcgt gagttacatc aagctggatg ccgcaacgat gcagcaaccg
    10741 gttagcgatg cggatatcca gagctactac gatcagcatc aggatcaatt cacccagccg
    10801 cagcgtaccc gctacagcat catccagacc aaaactgaag atgaagcgaa agcggtactt
    10861 gatgagctga ataaaggcgg tgattttgct gcattagcca aagaaaaatc tgccgatatt
    10921 atctctgccc gtaacggcgg cgatatgggt tggttagaag atgccactat ccccgacgaa
    10981 ctgaaaaatg ctggtctgaa agaaaaaggg caactgtctg gtgtcatcaa atcttcggtc
    11041 ggtttcctga ttgtacgtct ggacgacatt cagccagcga aagtgaaatc gttagacgaa
    11101 gtacgtgacg acattgcggc gaaagtgaaa cacgaaaaag ccctcgatgc gtactatgcg
    11161 ctgcagcaga aagtgagcga tgcggcaagc aacgacaccg agtctctggc cggtgcagag
    11221 caagctgccg gcgttaaagc cactcagacg ggttggttca gcaaagataa cctgccggaa
    11281 gagttgaact tcaagccggt tgccgacgct atctttaacg gcggtctggt aggtgaaaac
    11341 ggcgcgccgg gcatcaactc tgacatcatc accgtagacg gcgaccgcgc attcgtgctg
    11401 cgcatcagcg agcacaaacc ggaagcggtg aaaccgttgg cagatgttca ggaacaagtt
    11461 aaggcattgg ttcagcacaa caaagctgaa caacaggcga aagtggatgc tgagaaactg
    11521 ctggttgatt tgaaagccgg caaaggtgcg gaagctatgc aggctgccgg tctgaaattt
    11581 ggcgagccga aaaccttaag ccgttccggt cgtgacccga ttagccaggc ggcgtttgca
    11641 ctgccactgc cagcgaaaga caaaccgagc tacggtatgg cgaccgatat gcaaggtaat
    11701 gtggttctgc tggcgctgga tgaagtgaaa caaggttcaa tgccggaaga tcagaaaaaa
    11761 gcgatggtgc agggtatcac ccagaacaac gcacaaatcg tctttgaagc tctgatgagt
    11821 aacctgcgta aagaggcgaa aatcaaaatt ggcgatgcgc tggaacagca ataatcctga
    11881 agccgcctcg caaaaaaatg cgttgcagct gtaacaagaa aaggtcgctt tcgcggcctt
    11941 ttccatttct gaacattgcc atttgtttac tgttttcact gccgttaagg tgattccact
    12001 gttaacaaac aaggagaaaa cagtatgaaa cacggaatta aagcactgct cattaccctg
    12061 tccctggcct gtgccggaat gtctcatagc gcgctggcgg cagcttctgt ggcgaaaccg
    12121 acaacggtag aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg
    12181 aaagccagtg acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta
    12241 gcccgcgcga tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa
    12301 gagtacggtc cgtttaaaat ggtggaggat ctaaagcagg tgccggggat gggcaattcg
    12361 ctggtggaac gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt
    12421 tgccagactg aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt
    12481 tattgcgcta tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac
    12541 gtcaacaacg cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat
    12601 agcgacagtt ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat
    12661 attaactatc gtcgcccagc ggtattaagt gacctgttaa ctattaccag tcagttgcag
    12721 caattaaacg gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag
    12781 gtggtagcgg atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta
    12841 gctctggaag gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt
    12901 gccggatgct caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg
    12961 cttcatcgct gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg
    13021 taaattacat gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa
    13081 cgtttcgtta cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc
    13141 tttatcaatc cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac
    13201 ggcatggttt agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt
    13261 ttcacagacg ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag
    13321 cgtcaggaac aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg
    13381 ttcataatca ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag
    13441 cagcgttaca tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc
    13501 cacgtcgatt tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc
    13561 atattgttgt aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac
    13621 gacagcacgt ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg
    13681 attaacccgg accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa
    13741 accagccgcg cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt
    13801 gatagttgaa cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca
    13861 gggcattaaa tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca
    13921 gatgtgcata tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt
    13981 gctccagagt atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt
    14041 gtgccagtag ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac
    14101 agccttccgc tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta
    14161 ggtgataaac cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg
    14221 gaatagtcca gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta
    14281 acgtttgtaa taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg
    14341 agagtcgggg acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga
    14401 cctggctgac ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac
    14461 aactggttcc caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa
    14521 gcagcggatg acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga
    14581 attgtgttaa gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc
    14641 tgcaatagct cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca
    14701 gacactgcgg atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg
    14761 gcagttgtaa cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt
    14821 tatgccagtg tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt
    14881 cccagtgatg cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc
    14941 cggaaaatat ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta
    15001 gcggttcgag cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc
    15061 cgcccataaa cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca
    15121 gcacatcttg ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt
    15181 ccggcgtgac cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact
    15241 ccagccatcc cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa
    15301 aacagcgttc ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc
    15361 acagacgttg atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta
    15421 tttaactatt cactattact tccgtatata tcaggtgata ctcaatcacc attaaccgtg
    15481 tcacagagtg gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt
    15541 gatgcctgac catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg
    15601 cgacattacc ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg
    15661 ggcgctatcg ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga
    15721 aggtgaactt ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca
    15781 gcaatgggat acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa
    15841 agagatccct gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga
    15901 tgtcaaaaaa atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga
    15961 tcttacacgc ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt
    16021 ttccgccacg gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac
    16081 ggtgctgacc caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat
    16141 gaacgatcgc gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc
    16201 gcaactgcgc gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt
    16261 ctctcactat ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg
    16321 agatcccttc cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg
    16381 ccgtggtgtt taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa
    16441 aagagaaaaa aattctctgt ggaagggcta tgttagataa aattgaccgt aagctgctgg
    16501 ccttactgca gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga
    16561 caaccacccc ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca
    16621 aagtcgccct gctggatccg gaaaaaatag gcctcggcct gaccgctttt gtgctgataa
    16681 aaacgcaaca tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc
    16741 cagaagtgct ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg
    16801 ttgccgacat gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc
    16861 tgtcggacgt cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca
    16921 tcgaataaat atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag
    16981 ctggtatttc cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat
    17041 cgcgatgctg caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga
    17101 acaacacttt actaccgggc agatcctgat gtggatcgcc accatggtgc tgattgccgt
    17161 tgtggtttat ctcctgcgtt acgtctggcg ggtattgctg tttggtgcgt cttatcaact
    17221 ggctgttgaa ctgcgtgaag attattaccg tcagctaagc cggcagcatc ctgagtttta
    17281 cctgcgtcat cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg atcgcgtcgt
    17341 gtttgccgcc ggagaagggg tgctgacgct ggtggattca ctggtgatgg gctgcgctgt
    17401 gttgattatg atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat
    17461 gccagtgatg gcgatcatga tcaagcgcaa cggcgatgcc ttgcatgaac gctttaagct
    17521 ggcacaggcg gcgttttcta gtcttaatga ccgcacccag gaaagcctca ccagtatccg
    17581 catgatcaaa gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga
    17641 agataccggc aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccaaccat
    17701 ctatatcgcg attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggc
    17761 gcagggcagt ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat
    17821 ttggccaatg ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta
    17881 cagccgtatt cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt
    17941 gccggaaggg cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga
    18001 ccatcctgcg ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg
    18061 cgggccgact ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt
    18121 cagcgagggg gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg
    18181 gcgtagccgc ctggcggtag ttagccagac accgttcctt ttttccgaca ccgtagcgaa
    18241 caacatcgcg ttgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt
    18301 agccagcgta catgacgata ttttgcgtct accgcaaggt tacgatacag aagtgggcga
    18361 gcgcggcgtg atgctttccg gtgggcaaaa acagcgtatc tccattgctc gagcgttatt
    18421 agtcaacgcg gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga
    18481 gcaccagatc ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc
    18541 ccatcgcctt tcggcactga tggaagccag tgaaattatt gtgatgcagc acggacatat
    18601 cgcccagcgt ggcaatcatg atgagctggc gcaacaaagc ggctggtatc gcgatatgta
    18661 tcgctatcaa caactggagg cggcgctcga cgacgctccg gaaattcgcg aggaggccgt
    18721 cgatgcgtag ttttagccaa ctgtggccga ctctcaagcg tctgttagcg tacggttcgc
    18781 cgtggcgtaa accgctgggg attgcggtcc tgatgatgtg ggttgcggca gcggcagaag
    18841 tcagtgggcc gctgcttatc agctatttta tcgacaatat ggtagtgaaa aataacctgc
    18901 cgttgaaaat ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg
    18961 ggctacatta cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt
    19021 tgcgtaccga cgtgatggat gccgcattgc gccagccatt aagcgagttt gatacccaac
    19081 ccgtcgggca ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg
    19141 ttaccgtagt ggcaactgtc ctgcgtagcg ccgcactggt gggcgcgatg ctggtggcga
    19201 tgttcagcct cgactggcga atggcgctgg tggcgataat gattttcccg gtggtgctgg
    19261 tggtaatggt gatataccag cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc
    19321 tagcggatat caacgacggc tttaacgaaa tcatcaatgg catgagcgtt atccagcagt
    19381 tccgtcagca ggcgcgattt ggcgaacgta tgggggaggc cagtcgttca cactatatgg
    19441 ccaggatgca aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt
    19501 catcgctcat tctttgtggc ttgttgatgc tgtttggctt ctccgctagc ggcaccattg
    19561 aagtgggcgt gctgtatgcg tttatcagct atcttgggcg acttaacgaa ccattaatcg
    19621 aactgaccac gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg
    19681 aactgatgga cggaccgcgc cagcaatatg gcaatgatga tcgcccgtta cagagtggca
    19741 ccatcgaagt cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca
    19801 ttaatctctc tgtgccttcg cgtaactttg tggcactggt cgggcatacc ggcagtggca
    19861 aaagcaccct cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgtc
    19921 ttgatggtcg tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg
    19981 tgcagcaaga tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcgtg
    20041 atatctccga agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc
    20101 gcagcatgag tgatggtatt tatacgccgc tgggcgagca ggggaataat ctctccgtcg
    20161 ggcaaaagca actgctggca ctggcacgcg tgttggtgga gacgccgcaa atcctgatcc
    20221 ttgatgaggc aaccgccagc attgactccg gtactgaaca ggcgattcaa catgctctgg
    20281 cggctgtgcg tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg
    20341 atgccgacac cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc
    20401 aactgctggc ggcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag
    20461 agctggcagc cagcgtgcgt gaagaagaat cattgagcgc ctgaatagcg caatatttca
    20521 tcgttggtgc aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaatct
    20581 tcttaacttc ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc
    20641 ttcgtgtagc agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg
    20701 tgataatcaa accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc
    20761 agggcctgac cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt
    20821 accggggggc ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg
    20881 ctgatgacca actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa
    20941 ttggcgacgg caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg
    21001 aagccgacga agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat
    21061 agcgacgata aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc
    21121 acctgcggtg gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct
    21181 gtttatgact attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt
    21241 gctgtcgatg ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt
    21301 ttacggttac tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt
    21361 gatgctaaaa aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt
    21421 ggcgtttcag ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga
    21481 acgaatccgc ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat
    21541 tccgattgcg catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt
    21601 cgcgggtggc acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct
    21661 gataggcaaa cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt
    21721 cttcaccggt actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg
    21781 cacggcgaat gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc
    21841 aattcttggc tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc
    21901 gtgttctggc gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt
    21961 tggcggcgcg ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat
    22021 gctcaaacgc ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg
    22081 tggcattgtc ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg
    22141 cttcgctgaa ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat
    22201 tacgatcgtc tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt
    22261 tggtctgcgt gtaccggaag agcaggagcg agaagggctg gatgtcaaca gtcacggcga
    22321 gaatgcctat aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc
    22381 tattttttta attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagtacgc
    22441 cgtcctgggt ataaaactca ccgcgcacaa agccacgtgc gctggacgcc gaggtgctct
    22501 ccacgctata cagcagccat tcattcaaat taaacgggcg atggaaccac atggaatggt
    22561 caatggtggc aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta
    22621 ccggcaggaa gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat
    22681 ccggcacgct accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt
    22741 tcagtgggtt atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat
    22801 ctttaagcac tggcggcagc aggtgcgcca gcgattgggc gatttgcgtt tccgaaggca
    22861 ggccatcagg cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttctggtg
    22921 cctggaaaga ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc
    22981 gggcgctgaa gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac
    23041 tatcgccagg gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg
    23101 tctcttttgc agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac
    23161 ctaaatcttc actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg
    23221 tcagtaaatt ttttagcgcc tgactcatat aactctccag taacaaagct gccgcagcaa
    23281 gccaaagtga gttgagtata acgcaaattt gctactggtc cgatgggtgc aatggtctga
    23341 attacgggct aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat
    23401 tttgttaacc actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga
    23461 aatgaatgaa actcgtgcac atggccagtg gtttagcggt tgcgattgcg ttggcggctt
    23521 gcgcagataa aagcgcggat attcagacgc cagccccggc tgcaaatacg tctatttcag
    23581 caacacaaca acctgctatc cagcaaccga atgtctccgg taccgtctgg atccggcaga
    23641 aagtcgcact gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg
    23701 atgcaccgtc aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat
    23761 tcagctttgt tctgccattt aacccggcag atgttcagcc gaacgcgcgt attctgttga
    23821 gtgcggcgat taccgtgaat gacaaactgg tatttatcac cgataccgtt cagccggtga
    23881 tcaaccaggg cggaactaaa gccgacctga cattggtgcc ggtacagcaa accgccgtgc
    23941 cggttcaggc cagcggtggc gcaacgacta ccgtaccttc gacttcacca actcaggtga
    24001 atccgtcttc ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc
    24061 ggagagggtt agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc
    24121 atcacacctt ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa
    24181 attgtgccgt ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc
    24241 tttaacacac cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca
    24301 ccgtaagtgg tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga
    24361 aatgaatctt ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa
    24421 acgcccgagt gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat
    24481 tagccggggc agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc
    24541 tctgttggtt ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc
    24601 aaggcagatg acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc
    24661 caccgtttcg tcaaaaaaaa ccaacatggc taaactttaa ccacaactga cgtcgcaaga
    24721 attgtctggc tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg
    24781 tggttgccgt tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc
    24841 cattgtttaa agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg
    24901 atggcaaagt gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct
    24961 ttagctggat tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt
    25021 cggacccacg gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct
    25081 atggcggatt cgagtcaatc atcgacgaat agttagcgtg acgggctgtg cggcgtgccg
    25141 tcagcccaaa aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg
    25201 ccttgctgta gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt
    25261 gtttactggt ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga
    25321 tgatatgcgg cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca
    25381 gaatgtcgac atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat
    25441 caagataaat ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg
    25501 gatcggcaaa ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgatagt
    25561 gattgatcat ttcacgcagc aattgcggga ttttttccga ggtgagcacg gtggattcaa
    25621 gattgatcga aatatgctgc tgtggatgct gacgcagcca gtcgcccata tcttcaaaga
    25681 cgcttcttat aatcagtagc gtcaatggct cagaaaggcc cgtttgctga gccagcggaa
    25741 taaaactatc tggtgacaac caactaccgt ctgtctgcgg ccagcgcgcc agtgcctcag
    25801 caccgacaat tttgccattg gctaaggaga caatcggctg atagtgcacg caaatatcac
    25861 gattttcgat agcatcctgc agccgatgat gcggtgactg gatacggcgc aaaatacgca
    25921 gcacaaacat cgctgccagc aggccaatca ccaacccgag cggtaaccaa ataaagactt
    25981 gccgatgcca gcctttctgc aacattttcg ttgaagccca ggtgatgatc gaaatattca
    26041 tctccgggaa gggcaggata tcgtaaatga ttccattatt ttcgatatgc tcaccgggtg
    26101 ttttttgtag cctggtaata attccctgag caatttcatc gctgctggtt atgacaacgt
    26161 tatgggcatt gccaataatg gcggcatcaa tttgccatga gctataggga atgacatcaa
    26221 taaaggaagc ggggtcgatc atgacgacat aatgtgccgt tcccatggcg accatgtaac
    26281 ggataatgcc taaatcgtta tgcgatgtta accagacacg ataaccatct ttcgaaattt
    26341 tacctggctc ggggaaggta tcgggcggac tttcatgctc cagagacgaa cactggggaa
    26401 cgttgttatc gatataaacc acttcctgaa tatagcgata actgtaagat acccgacgca
    26461 tttccatgag atgggcttcg ctacaggcag cgccttgcca tctttccagc tcttgcagcg
    26521 catctttccc ttgtgtcgcc accttattgg cccgaatagc gacgcgggag gaataggtat
    26581 ccagctcttc aataaacgat gtttctacct gctgatgggc cagccagatg cttaagccga
    26641 caggcagcaa tactgaaaga atcagtactc ccgaaataag gccgaccaga tgtcgtgttc
    26701 tcaccagcat atccttatta acacagtgta gggaaagtat atctgattgt ggaaaaaggc
    26761 tggcaggaga taaggaggta gagctggtat ttcatgaagt gatgggtgga aacgatcatt
    26821 gtcatttgac caggcgcaga gctttacgcc tggtatgtaa ccctaactta agatgttgat
    26881 tcggcgcgag cgagggtaaa aatatcgtaa aaagacagtt cacctttacg gacgatcatt
    26941 ttttgcagtt gttccttatg aacatccgcg acacgcggag agttcaagat ggcttcaacc
    27001 agcgcggcag gtacaaagcg cgtgttgtac cattcgccgt tatagcaaac gcggaaatcc
    27061 agcacgtcga tatcttcata gcggtagcgt gggtagacat cgaaaaagaa gaaaccatta
    27121 agcaggataa ataatacaac cagtagccag acagagcctg acagcgtttc ggactgcaac
    27181 atcaccgcca gggtggcaaa aaaagcaacg tacataccga taaacagccc cggatgttta
    27241 cggataaaac tgatactaaa gcggggtttg ttgtcgcgtt tttcgcgggt attcagagac
    27301 tcaatcgttt cggtcagcag gcgttgtatt tcggtcatcg tttgccttcg tggtatctgc
    27361 aactttccag ggaacaccct attttaacgt gggaatagta gagaggaagt aacagattta
    27421 ccacaatttt gcataacagt tgcgaaaaac tgattacaat tttttaatta ttctggctgg
    27481 attaccgccc acgacaacgt tgtccgggac atcttttgtg acaactgcac ctgaggctac
    27541 cacgacgtta tcaccaatgg tcacaccagg gttaatgacc gcgcgtccgc caatccagac
    27601 gttattaccg atggtgacgg gtttccccag ttcagcaccg ctattacgtg ctacagggtc
    27661 gatgggatgt gttgccgtgt agatatgaac gcctggtgcc aacatacagt tatcaccgat
    27721 gcgaataggg cagacatcaa gcatcacgca atcgaagttg gcgaaaaaat tattaccgag
    27781 aaaaatgtta tagccatagt cacagcgaaa cgttggctca atataagcct ctgtcacctg
    27841 accgaataga tcagcgagaa tttgctggcg taatgtgtgc tcttccgcct gggaatgatt
    27901 gtatcggtga ataagctgac gagcgcgcag gcgatcgcga gataacgtct catctgccga
    27961 gcgatacaac tcaccagcaa tcatcttttc tttttctgtg ctcattactg acctccggtt
    28021 gctgaaaatg cggcaacaca gtaagacgaa aagtcagtga agagaacgtt cccattacat
    28081 ctgtgtgata aagatcacat agggtatgtc ttcatggtga aaagtataaa attcttaata
    28141 aacagccggt tatagctccg aaagcgaatt atttattagc gaataaattt ccatactgag
    28201 gaagggatct tgtcgtatag tttattcatg gtcaattcgg cgaggcggtg atctgcggct
    28261 gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc
    28321 tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa
    28381 ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata aactcgcagg
    28441 attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt
    28501 ctgaagatcc tgcatattaa taccataact actgaacaac ataaaggtgt catccagata
    28561 ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc
    28621 gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc
    28681 gttaacccag ccatgattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc
    28741 acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg
    28801 tccccttctt agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct
    28861 ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt tttcatcgca
    28921 ttattacgaa aaattacaat taggaaggaa tcgggacagg actcactttt tcatactagc
    28981 actaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt caggattatc
    29041 gccagatgaa attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag
    29101 gaatgaataa agaattaccg caaataatta agaatagcct ctaaatgatt atggattatt
    29161 aaatctatta gcgctactta atataaatta actaacaatc agtatttttt atgaatttta
    29221 tccgtggtta atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa
    29281 tgttcgtagg ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat
    29341 caatgatgat cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg
    29401 cgaaccacca caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc
    29461 ccgcccatta caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata
    29521 accagcggca taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc
    29581 atccgcactg catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct
    29641 ttggcgaatt cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg
    29701 cctacctgga agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg
    29761 ataaccccca gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc
    29821 gccgccagac acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg
    29881 ccggagaggc gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt
    29941 ttgctcgcca gttgttccat cagctccatt gcttcaccgg tacttttacc cggtgccgcc
    30001 tggcctaaga tttccatgga tggcaggcca ttgtaacgtt ccagacgcgg cgaaccgtac
    30061 tcccaacgag aagaggagaa tgccgagaac ggcaccatct gaccatcagc agcacgaaca
    30121 taccagtcgc cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact
    30181 ttcttcacac gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc
    30241 agagtggtgt taatgtcatt gatagaaaca cccagcgcct gcgctttttc ctggtcgata
    30301 tcaatcttaa actgcggggt atcttccaga ccgtttggac gtacgctggt caacatatca
    30361 gggtgcttcg ctgcttctgc aagcaactgg ttacgcgcct gagtcagttt ttcgtgacca
    30421 aggccagcct ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt
    30481 gcgggcaggt taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca
    30541 cgcatggtaa tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag
    30601 gaaacgaacg caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg
    30661 aacaccgact caacgttgtt tttttctttg gtcagatagt aatgcgttac ctcattgagc
    30721 actttctgcg tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg
    30781 ccctggtcct catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg
    30841 accacgatga tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg
    30901 ctgtcggtgt agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct
    30961 tttttacctt ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc
    31021 ggagtcagga tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag
    31081 aactgacgat agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgct
    31141 gacagtacca tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta
    31201 gcttcttttg gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg
    31261 atagcgtcat ccaccaacaa gccgatggcg agcaccatcc cgaacattgt tagcgtgttt
    31321 atcgagaagc caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca
    31381 atggtcggaa tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg
    31441 aggatgatcg cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc
    31501 gtggtgtcgt acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc
    31561 agttcagcac ggattgccgc agcggtatcc agcgcgtttg caccggtcgc cagcttgatc
    31621 cccagaccgg aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc
    31681 agctcaatct tcgccacatc acgcagcagt acgcgggaac catcctgatt cactttcagc
    31741 aggattttgc cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta
    31801 agctgttggc ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc
    31861 tgcgctttga tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc
    31921 gggttcatcc agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc
    31981 gacgtacggc tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc
    32041 gtcatggtgc catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc
    32101 tcaacgctca ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt
    32161 ttgttctgca cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg
    32221 atctgcacgg tacccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg
    32281 ttcatattct gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg
    32341 gggtaggagg cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc
    32401 agtttgagga tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa
    32461 atcgggcgat cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt
    32521 ggactgttca ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg
    32581 tgcttttacc tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc
    32641 tgctttcaga ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg
    32701 acgggtttcc actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt
    32761 acgggttacg ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg
    32821 tgcgcgcacg aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag
    32881 ggtgatagac ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg
    32941 cgggaactta atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt
    33001 cagcgtgcca ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac
    33061 atcaacgtag atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg
    33121 taccaatgcg ccttccgtca cgttcgactt accaatgcga ccgctaatcg gagaggtgac
    33181 tttggtgtaa gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc
    33241 agcattcgcc tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg
    33301 agtaccgagc agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg
    33361 ggctttcgcc agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat
    33421 ctgatagaga gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat
    33481 aatcccgcta acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag
    33541 ctcggttgtg atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg
    33601 ctggccacct tgttgggcct gtttgtcgtc acatcctgtt agggctaagc tgcctgagag
    33661 catcagaacg accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt
    33721 gtccgatttc aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta
    33781 tcgtcgtgct atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac
    33841 gaacatatgg cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat
    33901 gtggctctac gtcttttctc acagcagggg gtatcatcca cctcgctggg cgagattgca
    33961 aaagcagctg gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg
    34021 ttcagtgaga tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag
    34081 gcaaaattcc ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa
    34141 tccacggtga cagaagaacg gcgtcgatta ttgatggaga ttatattcca caaatgcgaa
    34201 tttgtcggag aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat
    34261 gaccgtatag aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta
    34321 atgacgcgtc gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg
    34381 ctctttgccc cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta
    34441 ctggagatgt atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga
    34501 atctgactcc aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg
    34561 tgatattctt gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat
    34621 catgactatg ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc
    34681 ttttgttttt gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga
    34741 tctgccgaca aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct
    34801 ttctgctcag gacaaactgg tgcagcagga tctgacagat acattagcca ccctcgataa
    34861 aatcgatcgc ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga
    34921 aaaaatgcgc caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga
    34981 aacgcgcaaa attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc
    35041 gctggacgat ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc
    35101 gttacagacg cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca
    35161 acaaattcgc agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca
    35221 gaaagtgtta atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa
    35281 aagcctggaa gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac
    35341 ggcgaacagc gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa
    35401 gcgcctgact ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg
    35461 tattcaggct aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg
    35521 tctgattacc gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa
    35581 ctggctggag cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa
    35641 gggcagcctg ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga
    35701 tgaactggaa aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa
    35761 ccagcagcgt gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca
    35821 caccaacgaa gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg
    35881 cgaattgctg gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct
    35941 gcaaatcaac cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca
    36001 gcaaatcttt tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc
    36061 gcaaagcctg aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg
    36121 gcctgccgtt tttatcgctt tcctcgccgg tttgccgctg ctgttgattg ccgggctgat
    36181 ccactggcgt ctgggctggc tgaaagcgta tcaacagaaa ctggcttccg ctgtgggttc
    36241 cctgcgtaac gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc
    36301 gctgccggtg tgcctgatta ttctcgcggt tggcctgatt ctgttgacca tgcagctcaa
    36361 catcagcgaa ctgctatggt cgttcagtaa aaaactggcg attttctggc tggtgtttgg
    36421 cctgtgctgg aaggtactgg agaaaaatgg cgttgccgtg cgtcacttcg gcatgccgga
    36481 gcagcagacc agccactggc gtcggcaaat tgtccgcatc agtctcgcgt tgctgcctat
    36541 ccatttctgg tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg
    36601 gcaggcgatg attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg
    36661 cgaaagctgg cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc
    36721 gattatcccg atcgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg
    36781 cctctccggg cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca
    36841 gacggtattg cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc
    36901 gcgtcggcag aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac
    36961 cattgcactg gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc
    37021 gctgttcggt gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatct
    37081 cgacagcatc acgctctggc attacaacgg cactgaagct ggcgctgcgg tggtgaaaaa
    37141 cgtcaccatg ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat
    37201 tcgcaacctg cctggtttac tggaagtgct ggtgctctcg cgactgaata tgcgtcaggg
    37261 cgcgtcgtat gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac
    37321 ggtgttcgga tcgctgggcg tctcttggga taaactccag tggctggccg cagcattatc
    37381 cgtaggtctt ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat
    37441 tctgttcgaa cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac
    37501 ggtaagtaag atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat
    37561 catcccgaac aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac
    37621 tacgcgtctg gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa
    37681 agtgttactg aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt
    37741 cttctttacg gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga
    37801 actgcgtgac cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg
    37861 tgaaaacgac atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg
    37921 cgatgaggtg acggaagtca aacgcgacta caaaggcgat gacccgacgc cagcggtagg
    37981 gtaataacca aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt
    38041 tcatgccgga tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta
    38101 ttgcaggagc ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac
    38161 aatcttaggg gacatttagc ggccccattt atttctcact tttccgcctc atcatcgcgc
    38221 gttaatttct tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct
    38281 gggatctgat tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg
    38341 gcattttcca gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc
    38401 gattttttcc agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg
    38461 atgcagcgtt tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgttcggc
    38521 aaccatctca cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg
    38581 ggcaattttc ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc
    38641 gatggcttcc agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg
    38701 ctgctcaact gcatgacgaa gcgcagccag attatcgccc gcctcatcga gacaagcctg
    38761 cagtgttgtc gcacgggttt gaaaaaggtg tctgtcaaaa cgggcactta gcgtggcaaa
    38821 ttgcgctacc ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag
    38881 cagcagggcg gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag
    38941 ccactctgta agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg
    39001 ctggcggtag tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt
    39061 atcctgctgg cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg
    39121 taccgctcat ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc
    39181 ggcgtcaaac cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc
    39241 gtccagatgc catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat
    39301 atgtggcgaa ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca
    39361 gttgcaatta ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt
    39421 caaaggttaa acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa
    39481 caagcaggca tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca
    39541 tcaaaagcat tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac
    39601 tggaagaccc gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg
    39661 cgggcattac caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag
    39721 ctctgggtct gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa
    39781 ccatcagtga aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg
    39841 ccatcaaacc gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta
    39901 tcgaagcgac cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca
    39961 ttatcaacct gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct
    40021 acagccttgt cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg
    40081 gacagcacaa gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc
    40141 ttcgtgaatc caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg
    40201 aaaatggcgc ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact
    40261 ggcgaacggc ttgtcgttag ggcgtattca tcatgcttat cttttttccg gcacccgtgg
    40321 cgtcggaaaa acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat
    40381 taccgcgacg ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt
    40441 cgatctgatt gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct
    40501 ggataacgtc cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctca tcgacgaagt
    40561 gcatatgctg tcgcgccaca gctttaacgc actgttaaaa actcttgaag agccgccgga
    40621 gcatgttaag tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc
    40681 acgctgtctg caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcagcttga
    40741 gcacatcctc aacgaagaac atatcgctca cgagccgcgg gcgctgcaat tgctggcacg
    40801 cgccgctgaa ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagtgg
    40861 tgacggccag gtttcaaccc aggcggtcag tgcgatgctg ggtacgcttg acgacgatca
    40921 ggcgctgtcg ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat
    40981 taatgaagcc gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct
    41041 gttgcatcgt attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc
    41101 cattgagctg cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta
    41161 ctatcagacg ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg
    41221 cgttgagatg acgctgctgc gcgcgctggc gttccatcca cgtatgcctc tgcctgagcc
    41281 agaagtgcca cgccagtcct ttgcgcccgt cgcgccaacg gcagtaatga cgccaaccca
    41341 ggtgccgccg caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac
    41401 caccagccag gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc
    41461 aaaaaagagt gaaccggcag ccgctacccg cgcgcggccg gtgaataacg ctgcgctgga
    41521 aagactggct tcggtcaccg atcgcgttca ggcgcgtccg gtgccatcgg cgctggaaaa
    41581 agcgccagcc aaaaaagaag cgtatcgctg gaaggctacc actccagtga tgcagcaaaa
    41641 agaagtggtc gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga
    41701 actggcggcg aagctggcgg cagaagccat tgagcgcgac gcgtgggcgg cacaggtgag
    41761 tcaactttcg ctaccaaaac tggtcgaaca ggtggcctta aatgcctgga aagaggagag
    41821 cgacaacgca gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc
    41881 acagcaaaaa ctggctgaag cgttgagcat gttaaaaggt tcaacggttg aactgactat
    41941 cgttgaagat gataatcccg cggtgcgtac gccgctggag tggcgtcagg cgatatacga
    42001 agaaaaactt gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg
    42061 tcggttcttc gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca
    42121 gcttacgttc gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc
    42181 tgggtaacct gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag
    42241 agatcgcgca gctggaagtc accggcgaat ctggcgcagg tctggtaaaa gtgaccatca
    42301 acggtgcaca caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag
    42361 agatgctgga agacctggtg gctgcagcat tcaacgacgc agcacgtcgt attgaagaaa
    42421 cgcagaaaga aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga
    42481 tgccgttctg atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct
    42541 gccgggcgtt ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg
    42601 tagcggcggg atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg
    42661 cgccgattgc cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg
    42721 tcaggaaaac ggtcaaatct gtgtggtgga gagtccggcg gacatttacg ccattgagca
    42781 gacggggcag ttttcaggtc gttattttgt gttgatgggg cacctgtcac cgctggacgg
    42841 catcggtccg gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat
    42901 cactgaagtg atcctcgcca ccaatcccac ggttgaaggt gaagctaccg ctaactacat
    42961 tgccgagctt tgcgcgcaat atgacgtgga agccagccga atcgctcatg gcgtaccggt
    43021 tggcggcgaa ctggaaatgg tcgatggcac cacgctgtca cactcccttg ccgggcgtca
    43081 taagattcgt ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattctc
    43141 ccttgtcccc atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga
    43201 cctacatgaa aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc
    43261 tgatgatcca ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg
    43321 cctccgatgc ggcggacaag ctgcgtttcc gtgcgctatc taacccggac ctgtacgaag
    43381 gtgatggcga actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct
    43441 ccgataacgg cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta
    43501 aatccggtac caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc
    43561 tgatcggtca gttcggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgaccg
    43621 tgcgtactcg cgcggcaggc gaaaaaccag aaaatggcgt gttctgggaa tcggctggcg
    43681 aaggtgaata caccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc
    43741 atctgcgtga aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcattagca
    43801 aatactccga ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagacggcg
    43861 aaaccgttat ctcctgggaa aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt
    43921 cggaaatcac cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaacg
    43981 atccgctgac ctggagccac aaccgtgttg aaggtaagca ggagtacacc agcctgctgt
    44041 acattccgtc ccaggctccg tgggatatgt ggaaccgcga tcataaacac ggcctgaaac
    44101 tgtacgtcca gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc
    44161 gcttcgtgcg tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc
    44221 tccaggacag cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa
    44281 tgctggaaaa actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg
    44341 gcctggtact gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac
    44401 tgctgcgttt tgcttctact tataccgatt cttctgcgca gaccgtgtct ctggaagact
    44461 acgtttcccg catgaaagaa gggcaggaga aaatctacta catcactgca gacagctatg
    44521 cggcagcgaa gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc
    44581 tgctttccga ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac
    44641 cgttccagtc ggtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg
    44701 agagcgcgaa agaagcggag aaagcactga ctccgttcat cgaccgtgtg aaagccctgc
    44761 tcggcgagcg cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg
    44821 tttcgaccga cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc
    44881 agaaagtgcc agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac
    44941 gtgcggcaga tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc
    45001 aggcgctgct ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga
    45061 accagctgct ggtttcctga tgtcatgccg gatgaccttt gtgtcatccg gcatttttct
    45121 tttcatcatc tgcactttcc gcaaattatc tcgccattaa ccgtttcagc ctcaggtgcc
    45181 tttcttgagg caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac
    45241 attttaaggg gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag
    45301 ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata
    45361 tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg
    45421 atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc
    45481 aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag
    45541 acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg
    45601 aactgattgt tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt cgtgtttatc
    45661 acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga
    45721 ctacccgtaa agacgatcag gaagaaaccg tgcgtaaacg tctggttgaa taccatcaga
    45781 tgactgcacc gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg
    45841 cgaaagttga cggcaccaag ccggttgctg aagttcgcgc tgatctggaa aaaatcctcg
    45901 gctaattcga aagcgcgcac ggacagtccc ctcgccccct cggggagagg gttagggtga
    45961 ggggaacagg cccgcacaag caaacttatc agcaatctca ggccggatat tcattcggcc
    46021 ttttacaaaa acctcaatct gcgctattcc tttttctgat ttgacctctc acagcaatta
    46081 gttcttcttc ctcacttttc cgctacaatt atcaacaagt tgaatcgata agaggcggta
    46141 atgcgtcaga ctaaaaccgg tatcctgctg gcaaacctgg gtacgcccga tgcccccaca
    46201 cctgaagcgg taaaacgcta tctgaaacaa tttttaagcg acagacgcgt ggttgatacc
    46261 tcacggttgt tatggtggcc gttgctgcgc ggcgtgattt tgccgctgcg ctcgccgcgt
    46321 gtggcgaagc tgtacgcctc ggtctggatg gaagacggct cgccgctgat ggtttacagc
    46381 cgccagcaac agcaggcgtt ggcacaacgt ttaccggata cgcccgtagc gctgggcatg
    46441 agctacggct cgccatcact ggaaagcgcc gtcgatgaac tcctggcaga gcatgtagat
    46501 catattgtgg tgctgccgct ttatccgcaa ttctcctgtt ctacggtcgg tgcggtatgg
    46561 gatgaactgg cacgcattct ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt
    46621 gattacgccg ataaccacga ttacattaat gcactggcga acagcgtacg cgcttctttt
    46681 gccaaacatg gcgaaccgga tctgctgctg ctctcttatc atggcattcc ccagcgttat
    46741 gcagatgaag gcgatgatta cccgcaacgt tgccgcacaa cgactcgcga actggcttcc
    46801 gcactgggga tggcaccgga aaaagtgatg atgacctttc agtcgcgctt tggccgggaa
    46861 ccctggctga tgccttatac cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtaggt
    46921 catatacagg tgatgtgccc gggctttgct gcggattgtc tggagacgct ggaagagatt
    46981 gccgagcaaa accgtgaggt cttcctcggt gccggcggga aaaaatatga atatattcca
    47041 gcgcttaatg ccacgccgga acatatcgaa atgatggcta atcttgttgc cgcgtatcgc
    47101 taaagctgag cggtaaagaa ctgagcgccg tcgcgaagag cctcgtcggc ggttttcatc
    47161 attcgtgaat aatgcaaaaa ggcatgcagc gtacctgggt agagtttgaa ctcacagggc
    47221 tgctgatgcg ccgctaacgt ctggtaaagc agacggctgt catccagcag cggatcgaac
    47281 tccgccccgg caataaaaca gggcggaact tcgcgagtga gatcattatt aaacagacag
    47341 taatacggtg actcgcggtc cgcgtcgttg cttaaatacg cctcttcata catctgcaaa
    47401 tcctgttgcg ttaagccatc ccagacaccg cccaacagac gacgagtcac ggaatcccgt
    47461 aatccgtaaa gcccatacca cagcaaaaca cccgcaattt taccgcaatc gatctgttta
    47521 tcacgcaacc acaacgcact ggcgagcgcc agcatggcac ctgcggaatc accggcaaag
    47581 ccaatgcggg acatattgat ttgataatcc tccgcctgct ggtggaaata acaacaagca
    47641 gccacaattt cctctatcgc ttgcggaaaa cgcgcttcag gtgaaagggg gtaatcaata
    47701 ccaatcaccg tacattggct gtagcttgcc agcaggcgca tgatgcgatc gtgggtatcg
    47761 agattgccga gaataaaacc gcctccatgc aaataaaata gcgtcgccgg gctatctggc
    47821 tgcggacaaa agagacgtgt ttccacctgc ccatattttg ttggaaccat gtaagctctg
    47881 gttgccattt ctggagcgcc cgcattccag aatcggcgct caagcgtgta atactgtcgt
    47941 tgctcagcaa tcgttcccgt tgcgggccag gacggtaaat ccgactgaag agtattcaca
    48001 acggtcttca tttcagcaga aataaggtcc agaacaggta gtttgttttc cggcttcata
    48061 caaaaactcc tttcaaatta cgtcattgta aggaaccact gccatgaaaa tgcgatcccg
    48121 cctgctgata ttgaaactgg ctgcgtctcg cgcgctcccg tcagattgtg ttaacattcg
    48181 ccgctcagtt aaccacccgt aaaaacaacc atgaaatttc ccggtaaacg taaatccaaa
    48241 cattacttcc ccgtaaatgc acgcgatccg ctgcttcagc aattccagcc agaaaacgaa
    48301 accagcgctg cctgggtagt gggtatcgat caaacgctgg tcgatattga agcgaaagtg
    48361 gatgatgaat tcattgagcg ttatggatta agcgccgggc attcactggt gattgaggat
    48421 gatgtagccg aagcgcttta tcaggaacta aaacagaaaa acctgattac ccatcagttt
    48481 gcgggtggca ccattggtaa caccatgcat aactactcgg tgctcgcgga cgaccgttcg
    48541 gtgctgctgg gcgtcatgtg cagcaatatt gaaattggca gctatgccta tcgttacctg
    48601 tgtaacacct ccagccgtac cgatcttaac tatctacaag gcgtggatgg cccgattggt
    48661 cgttgcttta cgctgattgg cgagtccggg gaacgtacct ttgctatcag ccctggccac
    48721 atgaaccagc tgcgggctga aagtattccg gaagatgtga ttgccggagc ctcggctctg
    48781 gttctcacct cttatctggt gcgttgcaag ccgggtgaac ccatgccgga agcaaccatg
    48841 aaagccattg agtacgcgaa gaaatataac gtaccggtgg tgctgacgct gggaactaag
    48901 tttgtcattg ccgagaatcc gcagtggtgg cagcaattcc tcaaagacca cgtctctatc
    48961 cttgcgatga acgaagatga agccgaagcg ttgaccggag aaagcgatcc gttgttggca
    49021 tctgacaagg cgctggactg ggtagatctg gtgctgtgca ccgccgggcc aatcggcttg
    49081 tatatggcgg gctttaccga agacgaagcg aaacgtaaaa cccagcatcc gctgctgccg
    49141 ggcgctatag cggaattcaa ccagtatgag tttagccgcg ccatgcgcca caaggattgc
    49201 cagaatccgc tgcgtgtata ttcgcacatt gcgccgtaca tgggcgggcc ggaaaaaatc
    49261 atgaacacta atggagcggg ggatggcgca ttggcagcgt tgctgcatga cattaccgcc
    49321 aacagctacc atcgtagcaa cgtaccaaac tccagcaaac ataaattcac ctggttaact
    49381 tattcatcgt tagcgcaggt gtgtaaatat gctaaccgtg tgagctatca ggtactgaac
    49441 cagcattcac ctcgtttaac gcgcggcttg ccggagcgtg aagacagcct ggaagagtct
    49501 tactgggatc gttaagttat cgtcggttcg taggccagat aaggcgttca cgccgcatct
    49561 ggcatttggc tctcgatgcc cgatgcgacg ctggcgcgtc ttatcatgcc tacatatttt
    49621 tcatatttta catccggcaa ccaccgttta ccccgtcacc acttcacccg ccggtggcgt
    49681 ttccagcagt tccagcatag tacgggcaat ttcacgttca cccatcacta cctgattcgc
    49741 gccacgttcg gtgatatacg tcacttcatc gtcataatgg gcgcgggcga taatctcaat
    49801 atccgggttt ttcgcgcggg cagatgccac aatctcgcct gcttcataac cgttgggaat
    49861 cgtcaggatc agccattttg cacattccag atgcgccagt tgcataattt cttcgttcgc
    49921 cgcattgccc aatactgcgc ggaccccacg ctctcgcagc tcatcaacac gggttcgtga
    49981 cgtctcaatc accatcagcg gaatatcaga ggcgagcaat ttctccccca gcaggctgcc
    50041 tacacgaccg taacccacca gtagcgcatg gttgcaaata tccactggga tctgcttctc
    50101 ttcttcgatt gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg ccagatattt
    50161 ctccagtagt gcgaacagta ccgggttgag cataatcgac aggatcgccc ctgccagtac
    50221 caggttttgt ccggcctgcg gcagtaaatt caatgccatt cccagtcccg ccaggataaa
    50281 cgcgaactca ccaatctgcg ccaggctggc ggcgatggtt aatgccgtac gttgggagtg
    50341 accaaacagt cgcaccagga aaaatgcggc taacgactta ccaaacagaa taatcgccag
    50401 cgtcgccagc actgccagcg gttgctgaat cagaattaac ggatcaaaca acatcccgac
    50461 ggagacaaaa aacagcaccg caaacgcgtc gcgcaatggc agcgtatcgt gggcggcacg
    50521 gtgactcagt tcagactcgt tcagtaccat cccggcaaag aacgcaccga gtgcaaagga
    50581 gacatcaaac agctctaccg caccaaaggc aatccctaac gccagcgcca gcaccgacag
    50641 ggtaaacagc tcgcgagaac cggttgccgc gctgcgtgcc ataatccacg gcaccagacg
    50701 gcgacctacc agcatcataa tggcgataaa tgcgatcact ttgccgatgg tgatccccat
    50761 atcgactgca agagtggcaa agcccacatc gccctgttcc atcattcctg ccactgcggg
    50821 cagcaacacc agcgtcagaa ccattaccag gtcttccaca atcaaccaac cgatggcgat
    50881 ttgcccacgc tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta acaccacggt
    50941 actggcggtg gaaagacata aaccgaacac gataccggtc attaacgacc agcccagcac
    51001 ggcagagagc gccataccca gcagcgtcgc cacggctatc tgggcgatcg caccgggaat
    51061 ggcgatggcc tttaccgcca tcaaatcctt cagcgaaaag tgcaaaccga cgccaaacat
    51121 caacagaatg acgcccagtt cagccagttc cggcgcaagc ttggtatcgg caacaaagcc
    51181 cggagtgaat ggtcctgcca gcacacccgc taacagatat cccaccagag gagaaatacg
    51241 tagtttattg gccagcatgc cgaggataaa ggcgagcaca aggccgccaa caatggtggt
    51301 gataagcggg gtggcgtgat gcattccgtc tccttttcct ggtggttatt gtccattttt
    51361 ggccgggaaa accaaaatta caggtaatag tttatgacaa tttcattgat gatgttcatg
    51421 aataattatt gaattttgca gaaaaatgga attagctgca aaaaaagcac ggatcggaaa
    51481 acagaaggcg gtttaacaga ggaaaggctt atggcgactg taaaacgatg cagccaaagt
    51541 ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta tcaggcagga atatggtcaa
    51601 catccccaat agtggcagga aagcacagat tttatagact aactcgatgc tggtgtgatc
    51661 ggcgataagc cccagaactg ccgctcccag acctcccatg ccaaaagcaa aaccgaaaaa
    51721 gagtccagaa accataccga tacgtcccgg aagtagctcc tgagcgtaga ccagaatggc
    51781 agagaatgcc gaagcgagga taaatccaat aatcaccgtt aaaacccccg tccagtgcag
    51841 gctggcgtag ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc cccaaatcac
    51901 atatttccgt ccaattttat cccctacagg cccgccgatc accgtacctg ccgcaacggc
    51961 aaacaggaag gcaaacagat gaagctgggc attctggata gataatccga atttttgcat
    52021 cagataaaag gtgtaatagc tgctgatgct cgccatatag aaatatttcg agaaaatgag
    52081 gattaacaga atgctgactg ccagtaccac tttgttgcgc ggcagtggat tgattatcgt
    52141 cgctttggat tttcctttat tcattcggtg ctgtgccgag taccaacggc taatttgcgc
    52201 caacaccacg atcgccagca gtgccgcaag cacaaaccag gcaacgttgc ctttgccata
    52261 aggcgcgata atcaccgccg ccagcaaggg tcccagggaa ctgccaaagt tgccgccgac
    52321 ctgaaagata gattgcgcca ggccatgccg cccgccggaa gccatacggg ctacgcgaga
    52381 agattccgga tgaaagaccg atgaaccggt accgaccagc gccgccgcca gcagaactgc
    52441 gccaaaactg cccgccagcg caagcagcac cagaccactt aaggtaaagc acatgccaat
    52501 tggcaacgac cacggcatcg gatatttatc ggtccagtag ccgaccactg gttgcagtag
    52561 cgaagaggcg agctggaagg tgagggttat catgccaatc tgcataaatg tcagagaaaa
    52621 ttctgactga agcagcggat aaatcgccag aatcagcgat tggatcatgt cgttcagcag
    52681 atgtgagagg ctgatagcac ctaaaatacc aaacgatgtt cgggccttgg tcgttgacgc
    52741 agccgcgccc gccacaggct ggggttgttc actcattgcc ataggaaagt cactttttca
    52801 gggttgcgat gtaaagaatg atcttatttg tgattattac ccgactaaca tacctgtatg
    52861 cgtcgcctga aggaagtctc aacgccgaat acagaatttc taatctggat gcagatttat
    52921 cttcaccgga cgcagacttg tctatgatgt cgcgtcatac tatttttcaa cacgttgaaa
    52981 tcaggtcagg gagagaagta tgaaattatt gcagcggggc gtggcgttag cgctgttaac
    53041 cacatttaca ctggcgagtg aaactgctct ggcgtatgag caggataaaa cctacaaaat
    53101 tacagttctg cataccaatg atcatcatgg gcatttttgg cgcaatgaat atggcgaata
    53161 tggtctggcg gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg cggctgaagg
    53221 cggtagcgtg ctgctacttt ccggtggcga cattaacact ggcgtgcccg agtctgactt
    53281 acaggatgcc gaacctgatt ttcgcggtat gaatctggtg ggctatgacg cgatggcgat
    53341 cggtaatcat gaatttgata atccgctcac cgtattacgc cagcaggaaa agtgggccaa
    53401 gttcccgttg ctttccgcga atatctacca gaaaagtact ggcgagcgcc tgtttaaacc
    53461 gtgggcgctg tttaagcgtc aggatctgaa aattgccgtc attgggctga caaccgatga
    53521 cacagcaaaa attggtaacc cggaatactt cactgatgtc gaatttcgta agcccgccga
    53581 tgaagcgaag ctggtgattc aggagctgca acagacagaa aagccagaca ttattatcgc
    53641 ggcgacccat atggggcatt acgataatgg tgagcacggc tctaacgcac cgggcgatgt
    53701 ggagatggca cgcgcgctgc ctgccggatc gctggcgatg atcgtcggtg gtcactcgca
    53761 agatccggtc tgcatggcgg cagaaaacaa aaaacaggtc gattacgtgc cgggtacgcc
    53821 atgcaagcca gatcaacaaa acggcatctg gattgtgcag gcgcatgagt ggggcaaata
    53881 cgtgggacgg gctgattttg agtttcgtaa tggcgaaatg aaaatggtta actaccagct
    53941 gattccggtg aacctgaaga agaaagtgac ctgggaagac gggaaaagcg agcgcgtgct
    54001 ttacactcct gaaatcgctg aaaaccagca aatgatctcg ctgttatcac cgttccagaa
    54061 caaaggcaaa gcgcagctgg aagtgaaaat aggcgaaacc aatggtcgtc tggaaggcga
    54121 tcgtgacaaa gtgcgttttg tacagactaa tatggggcgg ttgattctgg cagcccaaat
    54181 ggatcgcact ggtgccgact ttgcggtgat gagcggaggc ggaattcgtg attctatcga
    54241 agcaggcgat atcagctata aaaacgtgct gaaagtgcag ccattcggca atgtggtggt
    54301 gtatgccgac atgaccggta aagaggtgat tgattacctg accgccgtcg cgcagatgaa
    54361 gccagattca ggtgcctacc cgcaatttgc cagcgttagc tttgtggcga aagacggcaa
    54421 actgaacgac cttaaaatca aaggcgaacc ggtcgatccg gcgaaaactt accgtatggc
    54481 gacattaaac ttcaatgcca ccggcggtga tggatatccg cgccttgata acaaaccggg
    54541 ctatgtgaat accggcttta ttgatgccga agtgctgaaa gcgtatatcc agaaaagctc
    54601 gccgctggat gtgagtgttt atgaaccgaa aggtgaggtg agctggcagt aatccgaaag
    54661 tgccggatgt ttgcatccgg cacaatgctt aatcgcggcg ggcgatatca gcaaatttgg
    54721 catcgaggat ctttgccaga tcgcctgccg ccagttcgat atccagtccg cgcttgccgc
    54781 cggaaacata aatagtggca aattcttgtg cgggggcgtc gataatcgtt ggcagacgtt
    54841 ttttctgccc cagtgggcta atccccccaa ccaggtatcc cgtcgaacgc tgcgcgacca
    54901 tcggatcggc catctcaact ttcttggcac ccagcgcttt tgctactttt ttaagatcca
    54961 gttgacctgc gaccggcgta acgtccacgg caaggtgttt catatcaccg ttcactgcca
    55021 ccagcagcgt tttgtagacc tgatccggat tcaaacctaa ttttttgacg acttcatcgc
    55081 caaaattggt ttcagccgga tcgtgctcgt aggtatggat ttgaaacgaa atcttgtttt
    55141 tttcgagtaa tttaactgcg ggtgtcatgg taattttcct gccttaaaca aaaaaacgaa
    55201 gcaagcatac gcctgatggc ggtccaaaaa aaagagtcat cttgcctaag agtattggca
    55261 ggatggtgag attgagcgac aatcgagtta caccgtcgct taaagtgacg gcataataat
    55321 aaaaaaatga aattcctctt tgacgggcca atagcgatat tggccatttt tttagcgcaa
    55381 catttgcggc aaattccctt ctccatacag gtgtagtgca ccgaccgcga ccacatatcg
    55441 ccccggcggc atggcgcgta atttatcccg ccaggcgaga tttcgctgat gcatcagcac
    55501 atcgtacagc gactgactga acgtattggg cagcgttata tcattatttt gcggcggtgc
    55561 attcagccac cagctcatca tttgttgcag caaccgtgcg ttggtatgcc agtgggtcag
    55621 cgtatcgtcc agcagcgcca gtcctttgtc agggagctgg agcaacatgg caatctggtt
    55681 ttcagcccct tccagttcaa tcacgggttt atgttgttgc ttcgccgcct gcaataactg
    55741 gtaatcgata ccgtattctg cccgcagccc cagtttttgt gcctgcgtcg cctgaagaac
    55801 catcgcgatt tgccacagcg gttgagtaga aaagagcgac ggagaaatgc ccatctcctg
    55861 gctaatgtgc tgcagatttt ggagttgctc ctcgttaatg cgctcttcca gcgcctcgca
    55921 agtaggcaaa ttagcaaaag gcgtatcgct ggtggaaaca tccgcctcga cgatcagcgc
    55981 atcggcgttt ttgagctttt tgagcaaacg ggtgggcagg ggagccatat cgtggctacc
    56041 catatgaata ctgccaatca gatgaaaatg gcgattgccg ggaagggtga tatcgatggc
    56101 aggccaggtg taatgattac cgcgcagcgc ggcccaaagc gtttttaccc ggtacaacag
    56161 atccatacga cctccctttg tgaaatatca tgctagcgcg cggtgaggga tggcgcaaat
    56221 ggagttgggg acttgtttaa tgtgtttgta tgattcagta tgttcttgca tcgctattca
    56281 cagggaagca acagttaaaa accatgaaac aggcaacaag aaaaccgacg acacccggag
    56341 atattcttct ctatgaatat ctggaaccgc tcgatttgaa aatcaatgag ttagcagagt
    56401 tgctgcatgt tcatcgtaat agcgtcagtg cactgatcaa taacaatcgt aaactcacca
    56461 ctgagatggc attccgtctg gcgaaagttt ttgataccac agtcgatttt aggctaaacc
    56521 tccaggcggc agttgatctt tgggaagttg aaaacaacat gcgcacccag gaagaattgg
    56581 gacggattga aacagtggct gaatatttgg cacgccgtga agagcgtgca aaaaaggtcg
    56641 cgtaaggcca aaacatcgct tcatctcgta ggccggataa ggcgtttacg ccgcatccgg
    56701 cagtcatgcg tcgatgccaa atgtgccacc ctaaagcagc gcatccgcaa tgacatactt
    56761 attccttcgg tttaaaccgc agcaaccggt tggcgttact cactacggta atcgacgaga
    56821 gcgccattgc cgctccggca actaccgggt taagcagtgt tccagtaaac ggccacaaaa
    56881 taccggcggc gaccggaata ccgatactgt tgtagataaa ggcaccgagc aggttctgct
    56941 tcatgttgcg cagcgttgcg cgggaaatcg cgagcgcatc cgcaacgccc atcaggctat
    57001 ggcgcatcag ggtaatcgcc gcggtttcaa tggcaacatc actgccgccc cccatcgcaa
    57061 taccgacatc cgcctgggcc agcgctggcg catcgttaat gccgtcgccc accattgcca
    57121 cctgacgtcc ttcactttgc aggcgtttaa tcgcttcggc tttaccgtcc ggcagcaccc
    57181 cggcgatcac ttcatcaatc ccggcttctt tggcgatcgc attggcggtg gttgggttat
    57241 ccccggtcaa catcaccaga cgatatcccg ctttatgcag gcgttgcagc gccgccacgc
    57301 tatcactacg caatggatcg cgtactgcca gcagggctac cgctttcccg tcaaccgcca
    57361 gcagcacagg cgttgcccct tgcgatgcct gagcagtaat ctccgcttcg atagctttgg
    57421 taccaacctg ttgctcattt aacagcgcct gattgcccag caataacgca tgaccttcag
    57481 cttcaccgct cacgcccagc ccgcgcaatg tgcggaaacc gttgacctgc ggcagctgca
    57541 tatcacctgc tttatcgagg atcgcccgtg ccagcggatg gcaggaacct tgctccagtg
    57601 ccgccgccag acgcaatgcc tgcgcttcat caacatcagc aaacgttttc actgcgacaa
    57661 cctgcggctt cccttcggtc agcgtcccgg ttttatcgaa cacgacggta tcgagtgtac
    57721 tggcgcgttg cagcgcgtcg gcgtcccgca ccagcacgcc aaactcagcc gcccgcccga
    57781 cgccggaaat aatcgacatc ggcgtcgcca gccccagcgc acacggacag gcgataatca
    57841 gtaccgtggt ggcaatcacc agggtataaa caatttgcgg tgccggacca aagaagtacc
    57901 agattgccgc actgaccagc gcaatcacca ccaccacagg tacaaatacg gctgagattt
    57961 tatccgccag ctgaccgatt tctggcttgc tgctctgggc ctggcgcacc atgcgaatga
    58021 tccgtgacag cgtggtatgg ctgccaaccg cactggcacg aaacagcacg ctaccgtcct
    58081 gtaccactgt cccggcatgg acgctttcgc cttcgccttt ttgctgcggg attggttcgc
    58141 ccgtcagcat cgcttcatcc agccatgctt cgccctgggt aatctcgcca tctaccggca
    58201 cgcgatcgcc ggtcgtcagg cgcagcaaca tacctggctg cacttccgcc agaggcacgc
    58261 ttttttcacc ttcgtcagta accaggcgcg ccgtcggtgg ggttaaatcg agtaatttct
    58321 ccagcgcctt agaagaacgc tgacgtgcgc gtgcttccag catatggccg agattgatca
    58381 gaccgataat catcgcgctg gcttcgtaat aaagatgtcg cgcttccatc gggaaccact
    58441 gcggccacag gttgacgctc atcgaataga gccacgccac gccagtaccc agcgccacca
    58501 gcgtatccat cgtcgctgca ccgttcagca ggcttttcca tgcactgcgg taaaaatggc
    58561 cgccggcgaa aaccatcacc gccagggtta tcaggccgat aaccaaccac aggctgcggt
    58621 tgtcagcggt gaccatcatg ttatcgccaa tcatccccca gaccatcacc gggataccca
    58681 ccgccagtgc gacaattgcc tgccagcgga agcgcttcat cgtagcaacg gcggtttcct
    58741 gctggcgctc acggcgtttc gcgtcatctt caatcgcttc cgcgccgtag cccgcttttt
    58801 ccaccgcctg cactaaatct tgtggggagg cactgcccat caccagcgca gtacgctccg
    58861 ccaggtttac ccgtgcctga gtgacgcccg gtacgctttg cagcgcattt tgtacgcggg
    58921 tgacacagct ggcgcagctc atgccgctca gcagcaactg ctggctgtca tcgtcatcgg
    58981 cggtcgctgc cggaagcgcc tcagaaaccg ctgtcagtgc ttccgacggg attgatgact
    59041 ccgccagcgg tttagccttt gggtggctta cagatgcgtc ataacccgct tgtttgatgg
    59101 tttcgatcag ctgttctgca ctggcagtcc cggtaacgtg cgcttcagtg atagacacat
    59161 ccgcttgttc cacatccggg cgctgttcga gactttcttt cacgcgttta acgcagtgac
    59221 cgcaggacag gccgtccagg gtcaggtcga tagtttgtga cataaaacac tcctttaaga
    59281 cagttttgac tggctgtgat aaaggttaaa ccttccagca aggggaaggt caagaaatta
    59341 ataaaccagg cgagtaaaag tccgtaaaga ttaaaaaatt ggctcaattt gcatcaggat
    59401 tagacattta tctctttgtt ttcctgaagt taagttgcgg gtgctaagtt aaatcaagat
    59461 gcctgaaaat cggcaccggg gtgaggaatt acctcccgca tctataaaaa ggagttaaca
    59521 aaagatgtta gatgcaaaca aattacagca ggcagtggat caggcttaca cccaatttca
    59581 ctcacttaac ggcggacaaa atgccgacta cattcccttt ctggcgaatg tacctagtca
    59641 actggcagca gtggctattg tgacctgcga tggcaacatc tatcgcgcgg gtgatagtga
    59701 ttaccgcttt gcgctggaat ccatctcgaa agtctgtacg ttagcccttg cgttagagga
    59761 tgtcggcccg caggcggtac aggacaaaat tggcgctgac ccgaccggat tgccctttaa
    59821 ctcagttatc gccttagagt tgcatggcgg caaaccgctt tcgccactgg taaatgctgg
    59881 cgccattgcc accaccagcc tgattaacgc tgaaaatgtt gaacaacgct ggcagcgaat
    59941 tttacatatc caacagcaac tggctggtga gcaggtagcg ctctctgacg aagtcaacca
    60001 gtcggaacaa acaaccaact tccataaccg ggccattgcc tggctgctgt actccgccgg
    60061 atatctctat tgtgatgcaa tggaagcctg tgatgtgtac acccgtcagt gctccacgct
    60121 cctcaatact gttgaactgg caacacttgg cgcgacgctg gcggcggggg gtgtgaatcc
    60181 gctgacgcat gaacgcgttc ttcaggccga caacgtgccg tacattctgg ccgaaatgat
    60241 gatggaaggg ctgtatggtc gctccggtga ctgggcgtat cgcgttggtt taccgggcaa
    60301 aagcggtgta ggtggcggta ttctggcggt cgtccctggc gtgatgggaa ttgccgcgtt
    60361 ctcaccaccg ctggacgaag aaggcaacag tgttcgcggt caaaaaatgg tggcatcggt
    60421 cgctaagcaa ctcggctata acgtgtttaa gggctgatca tgatgaacac ggaaggtaat
    60481 aacggtaaca aacctctcgg tctatggaac gtcgttt
//
LOCUS       DAZGXZ010000012        58559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000012 DAZGXZ010000000
VERSION     DAZGXZ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.012"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            326..1096
                     /gene="yjjP"
                     /locus_tag="ACHKJF_000868"
     CDS             326..1096
                     /gene="yjjP"
                     /locus_tag="ACHKJF_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP0948276.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1087..1560
                     /gene="yjjB"
                     /locus_tag="ACHKJF_000869"
     CDS             1087..1560
                     /gene="yjjB"
                     /locus_tag="ACHKJF_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP0948277.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1667..2206
                     /gene="dnaT"
                     /locus_tag="ACHKJF_000870"
     CDS             1667..2206
                     /gene="dnaT"
                     /locus_tag="ACHKJF_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP0948278.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2209..2946
                     /gene="dnaC"
                     /locus_tag="ACHKJF_000871"
     CDS             2209..2946
                     /gene="dnaC"
                     /locus_tag="ACHKJF_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP0948279.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2995..3489
                     /gene="yjjA"
                     /locus_tag="ACHKJF_000872"
     CDS             2995..3489
                     /gene="yjjA"
                     /locus_tag="ACHKJF_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP0948280.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3743..6034
                     /gene="opgB"
                     /locus_tag="ACHKJF_000873"
     CDS             3743..6034
                     /gene="opgB"
                     /locus_tag="ACHKJF_000873"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP0948281.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRHR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6173..7195)
                     /gene="lgoD"
                     /locus_tag="ACHKJF_000874"
     CDS             complement(6173..7195)
                     /gene="lgoD"
                     /locus_tag="ACHKJF_000874"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP0948282.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKKIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7334..8248
                     /gene="lgoR"
                     /locus_tag="ACHKJF_000875"
     CDS             7334..8248
                     /gene="lgoR"
                     /locus_tag="ACHKJF_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP0948283.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8463..9824
                     /gene="lgoT"
                     /locus_tag="ACHKJF_000876"
     CDS             8463..9824
                     /gene="lgoT"
                     /locus_tag="ACHKJF_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0948284.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9873..11528)
                     /gene="tsr"
                     /locus_tag="ACHKJF_000877"
     CDS             complement(9873..11528)
                     /gene="tsr"
                     /locus_tag="ACHKJF_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP0948285.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(11647..12093)
                     /gene="hpaR"
                     /locus_tag="ACHKJF_000878"
     CDS             complement(11647..12093)
                     /gene="hpaR"
                     /locus_tag="ACHKJF_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFP0948286.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12365..13654
                     /gene="hpaG"
                     /locus_tag="ACHKJF_000879"
     CDS             12365..13654
                     /gene="hpaG"
                     /locus_tag="ACHKJF_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFP0948287.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13651..15117
                     /gene="hpaE"
                     /locus_tag="ACHKJF_000880"
     CDS             13651..15117
                     /gene="hpaE"
                     /locus_tag="ACHKJF_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0948288.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEDIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15119..15970
                     /gene="hpaD"
                     /locus_tag="ACHKJF_000881"
     CDS             15119..15970
                     /gene="hpaD"
                     /locus_tag="ACHKJF_000881"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFP0948289.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYDGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15980..16360
                     /locus_tag="ACHKJF_000882"
     CDS             15980..16360
                     /locus_tag="ACHKJF_000882"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFP0948290.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16428..17231
                     /gene="hpaH"
                     /locus_tag="ACHKJF_000883"
     CDS             16428..17231
                     /gene="hpaH"
                     /locus_tag="ACHKJF_000883"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFP0948291.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17242..18030
                     /gene="hpaI"
                     /locus_tag="ACHKJF_000884"
     CDS             17242..18030
                     /gene="hpaI"
                     /locus_tag="ACHKJF_000884"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFP0948292.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            18205..19581
                     /gene="hpaX"
                     /locus_tag="ACHKJF_000885"
     CDS             18205..19581
                     /gene="hpaX"
                     /locus_tag="ACHKJF_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFP0948293.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            19591..20481
                     /gene="hpaA"
                     /locus_tag="ACHKJF_000886"
     CDS             19591..20481
                     /gene="hpaA"
                     /locus_tag="ACHKJF_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFP0948294.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFHMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLRETKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            20732..22294
                     /gene="hpaB"
                     /locus_tag="ACHKJF_000887"
     CDS             20732..22294
                     /gene="hpaB"
                     /locus_tag="ACHKJF_000887"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFP0948295.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            22312..22824
                     /locus_tag="ACHKJF_000888"
     CDS             22312..22824
                     /locus_tag="ACHKJF_000888"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFP0948296.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            23217..25367
                     /gene="btsT"
                     /locus_tag="ACHKJF_000889"
     CDS             23217..25367
                     /gene="btsT"
                     /locus_tag="ACHKJF_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP0948297.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLNVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            25417..25620
                     /gene="yjiX"
                     /locus_tag="ACHKJF_000890"
     CDS             25417..25620
                     /gene="yjiX"
                     /locus_tag="ACHKJF_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0948298.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            25631..26587
                     /gene="yjiA"
                     /locus_tag="ACHKJF_000891"
     CDS             25631..26587
                     /gene="yjiA"
                     /locus_tag="ACHKJF_000891"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0948299.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(26665..29205)
                     /locus_tag="ACHKJF_000892"
     CDS             complement(26665..29205)
                     /locus_tag="ACHKJF_000892"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFP0948300.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(29557..29721)
                     /gene="yjiS"
                     /locus_tag="ACHKJF_000893"
     CDS             complement(29557..29721)
                     /gene="yjiS"
                     /locus_tag="ACHKJF_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP0948301.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            29898..31310
                     /gene="yjiR"
                     /locus_tag="ACHKJF_000894"
     CDS             29898..31310
                     /gene="yjiR"
                     /locus_tag="ACHKJF_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0948302.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQSYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(31552..32496)
                     /locus_tag="ACHKJF_000895"
     CDS             complement(31552..32496)
                     /locus_tag="ACHKJF_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0948303.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVILLVTECANDSQITALLNYIL
                     LTGDEARFKKFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            32963..34195
                     /gene="mdtM"
                     /locus_tag="ACHKJF_000896"
     CDS             32963..34195
                     /gene="mdtM"
                     /locus_tag="ACHKJF_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP0948304.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            34236..35516
                     /gene="yjiN"
                     /locus_tag="ACHKJF_000897"
     CDS             34236..35516
                     /gene="yjiN"
                     /locus_tag="ACHKJF_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP0948305.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKLIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            35632..36783
                     /gene="yjiM"
                     /locus_tag="ACHKJF_000898"
     CDS             35632..36783
                     /gene="yjiM"
                     /locus_tag="ACHKJF_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP0948306.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDTSRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            36793..37560
                     /gene="yjiL"
                     /locus_tag="ACHKJF_000899"
     CDS             36793..37560
                     /gene="yjiL"
                     /locus_tag="ACHKJF_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP0948307.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            37557..37814
                     /gene="ytiA"
                     /locus_tag="ACHKJF_000900"
     CDS             37557..37814
                     /gene="ytiA"
                     /locus_tag="ACHKJF_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP0948308.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQSAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            37879..38739
                     /gene="yjiK"
                     /locus_tag="ACHKJF_000901"
     CDS             37879..38739
                     /gene="yjiK"
                     /locus_tag="ACHKJF_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0948309.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            38807..39985
                     /gene="yjiJ"
                     /locus_tag="ACHKJF_000902"
     CDS             38807..39985
                     /gene="yjiJ"
                     /locus_tag="ACHKJF_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0948310.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMNWWLLAIL
                     YGLAGFGYIIVATFLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(39998..40552)
                     /gene="kptA"
                     /locus_tag="ACHKJF_000903"
     CDS             complement(39998..40552)
                     /gene="kptA"
                     /locus_tag="ACHKJF_000903"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP0948311.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            40802..41485
                     /gene="yjiH"
                     /locus_tag="ACHKJF_000904"
     CDS             40802..41485
                     /gene="yjiH"
                     /locus_tag="ACHKJF_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP0948312.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            41482..41943
                     /gene="yjiG"
                     /locus_tag="ACHKJF_000905"
     CDS             41482..41943
                     /gene="yjiG"
                     /locus_tag="ACHKJF_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP0948313.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            41956..43128
                     /gene="iadA"
                     /locus_tag="ACHKJF_000906"
     CDS             41956..43128
                     /gene="iadA"
                     /locus_tag="ACHKJF_000906"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0948314.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            43193..44104
                     /gene="hypT"
                     /locus_tag="ACHKJF_000907"
                     /gene_synonym="qseD"
     CDS             43193..44104
                     /gene="hypT"
                     /locus_tag="ACHKJF_000907"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP0948315.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(44097..44489)
                     /gene="iraD"
                     /locus_tag="ACHKJF_000908"
     CDS             complement(44097..44489)
                     /gene="iraD"
                     /locus_tag="ACHKJF_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP0948316.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(44486..44569)
                     /gene="idlP"
                     /locus_tag="ACHKJF_000909"
     CDS             complement(44486..44569)
                     /gene="idlP"
                     /locus_tag="ACHKJF_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP0948317.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(44685..44828)
                     /gene="ytiD"
                     /locus_tag="ACHKJF_000910"
     CDS             complement(44685..44828)
                     /gene="ytiD"
                     /locus_tag="ACHKJF_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFP0948318.1"
                     /translation="MADYAEINNFPPELSSSGDKYFHLRNYSEYSEYTSGFFLSLMIF
                     IKS"
     gene            45162..45992
                     /gene="yjiC"
                     /locus_tag="ACHKJF_000911"
     CDS             45162..45992
                     /gene="yjiC"
                     /locus_tag="ACHKJF_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0948319.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(46133..46906)
                     /gene="uxuR"
                     /locus_tag="ACHKJF_000912"
     CDS             complement(46133..46906)
                     /gene="uxuR"
                     /locus_tag="ACHKJF_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP0948320.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(47121..48581)
                     /gene="uxuB"
                     /locus_tag="ACHKJF_000913"
     CDS             complement(47121..48581)
                     /gene="uxuB"
                     /locus_tag="ACHKJF_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP0948321.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRAKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(48662..49846)
                     /gene="uxuA"
                     /locus_tag="ACHKJF_000914"
     CDS             complement(48662..49846)
                     /gene="uxuA"
                     /locus_tag="ACHKJF_000914"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP0948322.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            50186..51529
                     /gene="gntP"
                     /locus_tag="ACHKJF_000915"
     CDS             50186..51529
                     /gene="gntP"
                     /locus_tag="ACHKJF_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP0948323.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(51772..52674)
                     /gene="fimH"
                     /locus_tag="ACHKJF_000916"
     CDS             complement(51772..52674)
                     /gene="fimH"
                     /locus_tag="ACHKJF_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0948324.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(52694..53137)
                     /locus_tag="ACHKJF_000917"
     CDS             complement(52694..53137)
                     /locus_tag="ACHKJF_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0948325.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(53210..53740)
                     /gene="fimF"
                     /locus_tag="ACHKJF_000918"
     CDS             complement(53210..53740)
                     /gene="fimF"
                     /locus_tag="ACHKJF_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP0948326.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(53751..56387)
                     /gene="fimD"
                     /locus_tag="ACHKJF_000919"
                     /pseudo
     CDS             complement(53751..56387)
                     /gene="fimD"
                     /locus_tag="ACHKJF_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(56454..57179)
                     /gene="fimC"
                     /locus_tag="ACHKJF_000920"
     CDS             complement(56454..57179)
                     /gene="fimC"
                     /locus_tag="ACHKJF_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0948327.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(57216..57755)
                     /gene="fimI"
                     /locus_tag="ACHKJF_000921"
     CDS             complement(57216..57755)
                     /gene="fimI"
                     /locus_tag="ACHKJF_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0948328.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(57820..58368)
                     /gene="fimA"
                     /locus_tag="ACHKJF_000922"
     CDS             complement(57820..58368)
                     /gene="fimA"
                     /locus_tag="ACHKJF_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0948329.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT        13853 a        15330 c        14771 g        14605 t
ORIGIN      
        1 tcccgcagaa tgaaaaatca cacaaccaat atttattcaa tgaaatctga tggttttaac
       61 ttgttaacat taaaaaacgt gcccggttat ctattatcct gattcgtaaa ggccaaacaa
      121 taaattcatt atagtgataa tattcatgtg atagtttgtt tgcggcgaga gataattcgc
      181 tttttatcac cgagcttgtc ccgataacca ggattaatca gcgcaatttt gatcttaacc
      241 taccgcaaaa caaccgtgct tcatgataca aatggattct cattatctga acaatacgca
      301 gcacgtctat gacaaagggc gagttatgca aactgagcaa cagcgagccg taacgcggct
      361 ttgtatccag tgtggattat ttcttttgca acatggtgcg gaaagcgcgt tggttgatga
      421 gctttcctca cgactgggtc gggcgctggg aatggacagc gttgaaagtt ctatctcttc
      481 gaacgccata gtgctgacaa ccattaaaga tgggcaatgc ctgacatcga cacgtaaaaa
      541 tcacgatcgc ggcattaata tgcatgtggt gactgaagtc cagcacattg tgattctggc
      601 ggagcaccat ctgctggatt acaaaggcgt agagaaacga tttagccaaa ttcagccatt
      661 acgttaccca agatggctgg tagccttaat ggttggcctt tcttgcgcct gtttctgtaa
      721 actcaataac ggtggctggg atggtgccgt catcaccttc tttgccagta cggccgcgat
      781 gtatatccgc cagctgctgg cacaacgtca tcttcatcca cagatcaact tttgccttac
      841 cgctttcgcc gccaccacca tttccggatt gcttttgcaa ctccccactt tcagcaatac
      901 ccccaccatt gcgatggccg ccagcgttct gctgctggtg ccgggctttc cgttgattaa
      961 tgccgtcgcc gatatgttta aaggccacat caataccgga ctggcacgct gggcgatcgc
     1021 cagtctgctg acactggcta cctgcgtcgg cgtagtgatg gcactgacga tttgggggct
     1081 acgcggatgg gtgtgatcga atttctgtta gcgttggcgc aggatatgat cctcgccgcc
     1141 attcctgcgg tcggctttgc gatggtgttc aacgttcccg ttcgggcgtt acgctggtgt
     1201 gcgctgcttg gcgcgattgg tcatggttca cgaatgatct taatgaccag cgggctgaat
     1261 attgagtggt caacttttat ggcttctatg ctggtcggca ccattggtat tcaatggtcg
     1321 cgctggtatc tggcgcatcc gaaagtgttt accgtggcgg ccgttatccc tatgttcccg
     1381 ggcatatcgg cttataccgc aatgatttcg gcggtaaaaa tcagccagtt aggttacagc
     1441 gaaccgttga tgattaccct gttaaccaac tttcttacag cttcatcgat tgttggtgcg
     1501 ttatccatcg gtctttccat tcctggatta tggttgtacc gcaagcgccc tcgcgtataa
     1561 aattgcctcg cttaagcaca cggatgagag acagcctcct ctcctccgtg tgttactata
     1621 aaagttatct cccttctcgt tcatcgttcc atatttgaga aacagtatgt cttccagagt
     1681 tttgaccccg gacgtcgttg gtattgacgc cctggtacac gatcaccaaa ccgttctggc
     1741 aaaagctgaa ggcggtgtgg ttgccgtatt tgctaacaat gccccggcgt tttatgccgt
     1801 cacgcctgca cgcctggctg aactgctggc gctggaagaa aagctggcgc gtccgggaag
     1861 cgatgtcgct ctggacgatc aactctatca ggaaccgcaa gccgctcccg ttgctgtccc
     1921 catggggaaa ttcgccatgt atccggactg gcaacccgat gccgacttta tccgcctggc
     1981 ggcgctatgg ggcgtggcgc taagagagcc ggtgaccacc gaagaactgg cctcattcat
     2041 tgcctactgg caggcggaag gtaaagtctt tcaccatgtg cagtggcaac aaaaactggc
     2101 gcgcagcctg caaatcggtc gtgccagcaa cggcggactg ccgaaacgag atgtgaatac
     2161 ggtcagcgaa cctgacagcc aaattccacc aggattcaga gggtaacgat gaaaaacgtt
     2221 ggcgacctga tgcaacgcct gcaaaaaatg atgcctgccc atatcaaacc tgcattcaaa
     2281 acgggtgaag agctactggc ctggcagaaa gaacaaggag cgatccgctc cgccgctctc
     2341 gaacgtgaaa atcgggcgat gaaaatgcag cgcaccttta accgctccgg tattcggcca
     2401 ctgcatcaga actgctcctt tgagaactat cgcgttgagt gtgaagggca gatgaatgcg
     2461 ttaagcaaag cgcgccagta cgtcgaagag tttgacggca acatcgccag cttcatcttt
     2521 tctggtaagc caggaaccgg caaaaaccat ctggcggcgg caatctgcaa cgagctgctg
     2581 ctacgcggta aatccgtatt gatcatcacc gtggccgata ttatgtcggc gatgaaagat
     2641 accttcagga atagcggtac cagtgaagaa caactgctta acgatctgag caacgttgat
     2701 ctgctggtga tcgatgagat cggcgtgcag accgaatcga aatacgaaaa agtgatcatc
     2761 aaccagatcg tcgatcgccg ctcttcttcc aaacgcccaa ccgggatgct gaccaacagt
     2821 aatatggaag agatgaccaa gttgctgggc gaacgcgtga tggaccgtat gcgcctgggt
     2881 aacagtttgt gggtgatctt caactgggat agctaccgta gccgggtaac aggtaaagag
     2941 tattaatccc cgtttctggc ggtatactgc gacacaattc caccttgagt aatgatgatg
     3001 aaaactgtta aacatcttct gtgctgtgcc attgccgcca gcgcattaat ttccaccggg
     3061 gtgcatgctg cgtcctggaa agatgcgctc tccagcgccg ccagcgaact tggcaaccaa
     3121 aacagcacga cacaggaagg cggttggtcg ctcgcgtcat taactaactt gcttagcagc
     3181 ggaaaccagg ccttaagcgc agataacatg aacaatgccg caggcattct gcaatactgc
     3241 gcgaagcaaa agctggcttc ggtaaccgat gccgaaaaca tcaagaacca gatgctggaa
     3301 aagctgggcc tgaacagtga agagcaaaaa gaagacacca actatctgga cggcattcag
     3361 ggtttgctga aaaccaaaga tggtcagcaa ctcaatctgg ataacatcgg aacgactccg
     3421 ctggcagaaa aggtgaaaac caaagcctgc gatctggtgt taaagcaggg gctgaacttc
     3481 atttcctgaa ccgcgaaacg ccgcgtttta gctgttaccc ggctgacgcg gctctctcct
     3541 gcccttttcc ctgctcctgg acggttttcc ccctgttaca cgggaagcga ccgcgatcaa
     3601 aaaagtctgt ttataaacta attcctaaca acatcgcatt tttttgacac tagaattgca
     3661 gcaatatggc gacgccatta cattgtgctt ctcaaaaagc ttaaacgttg gctaaacggc
     3721 ccggtttagt gaggatcgcc cgttgtcaga actactctct ttcgcccttt ttctcgcctc
     3781 tgtgctgatt tacgcatgga aagcgggacg taacacctgg tggtttgcag ccacattaac
     3841 ggtgctgggg ctatttgtcg ttttaaatat caccctgttt gccagcgact attttactgg
     3901 cgatggtatt aacgacgcgg ttctctatac cttaaccaac agcctgaccg gtgctggcgt
     3961 cagcaaatac atcctgccgg gtatcggcat tgtgctgggg ctgacagcgg tgttcggtgc
     4021 gctgggctgg atcctgcgcc gtcatcgcca tcatccgcac cattttggtt acagcctgct
     4081 ggcgctctta ctggcgctgg gttcggtgga cgccagcccg gcatttcgtc agataacgga
     4141 actggtgaaa tcccagtcac gcgatggcga cccggacttt gcggcttatt ataaagagcc
     4201 gtcgaaaact atccctgacc cgaaactcaa cctggtttat atctacggcg aaagtctcga
     4261 gcggacctat tttgataacg aagctttccc ggatctcacg cctgaactgg gcgcgttgaa
     4321 aaatgaaggc ctggatttca gccacacgca gcagctgcca ggaacggatt acacgattgc
     4381 gggcatggtg gcttctcagt gcggcatacc gctgtttgcc ccctttgaag gcaacgcctc
     4441 cgcctctgtc tccagcttct tcccgcagaa catctgtctg ggcgatatcc tgaaaaactc
     4501 gggttatcag aactatttcg tgcagggcgc gaatctgcgt tttgccggta aagatgtgtt
     4561 cctgaagtcg catggcttcg accacttata cggctcagaa gagctgaaaa gcgtggtggc
     4621 cgacccgcac tatcgcaacg actggggatt ctacgacgat accgttctcg atgaagcgtg
     4681 gaaaaagttt gaagagcttt cccgctcagg tcagcgattc tcactgttta ccctgacagt
     4741 cgatacccat cacccggatg gttttatctc gcgtacctgt aaccgcaaaa aatatgattt
     4801 tgacggtaag ccgaaccagt cattcagcgc ggtaagttgc agccaggaga atatcgcggc
     4861 gtttatcaac aaaatcaaag cgtcaccgtg gtttaaagat accgtcatcg tcgtctcttc
     4921 tgaccattta gcgatgaaca acacggcgtg gaaatacctt aataagcagg atcgcaataa
     4981 cctgtttttt gtcattcgtg gcgacaagcc gcagcaagag acgctggcag tgaagcgtaa
     5041 cacgatggat aacggcgcga cggtgctgga cattctcggt ggcgataact atctcggact
     5101 tggtcgtagc agtttatccg ggcagtcgat gtcggaaatc ttcctcaata tcaaagagaa
     5161 aacattggcg tggaagccgg atatcatccg cctgtggaaa ttccctaaag agatgaaaga
     5221 gttcactatc gaccagcaga aaaacatgat tgccttctcg ggtagccatt tccgtttgcc
     5281 gctgctgttg cgggtttcag acaagcgcgt agaaccgctg ccggaaagtg aatactcagc
     5341 accgctgcgt ttccagctgg ccgatttcgc tccacgcgac aatttcgtct gggttgaccg
     5401 ttgctacaag atggcacaac tctgggctcc ggaactggca ctctccaccg actggtgtgt
     5461 ctcgcagggg cagcttggcg gtcagcaaat tgtccagcat gttgataaaa caatgtggaa
     5521 gggcaaaacg gcatttaaag atacggtcat cgacatggcg cgttacaaaa gcaatgtcga
     5581 tacgctgaaa attgttgata acgatattcg ctacaaagcc gacagtttca tcttcaacgt
     5641 cgccggtgcg ccagaagagg tgaaacagtt tagcgggatt tcccgtccgg agtcgtgggg
     5701 ccgctggtcc aacgctcagc tgggcgatga agtaaaaatc gagtacaagc atccgctgcc
     5761 gaagaaattt gacctggtga ttaccgccaa agcatacggc aataacgcca gccgtcctat
     5821 tccggtacgc gtaggcaatg aagaacaaac tcttgtgctg ggcaatgaag taaccaccac
     5881 cacactgcat ttcgataacc caaccgatgc cgacacactg gtaattgtgc cgccggaacc
     5941 tgtctcaacc aacgaaggga atatcctcgg acactcgccg cgtaagctcg ggatcggcat
     6001 ggtggaaatt aaagttgtgg aacgtgaagg gtaatttctg actgatgtgt aggtcgcata
     6061 aggtgcgacc tacacacctt ctccggccta caaaacatca ttgagaaaca gaaaaaaaca
     6121 gcgcccaaca cttccccgga cgctgcatgg caactttaat aatctcgcct tatcagaaag
     6181 taattacacc cttaatcaac tcacgattgt taatcacatc gcgctcgtag gtttctgcca
     6241 gcgtggcgaa cggatagcga tgggttaaca tcatgtcggc agtgattttc ccttctgcca
     6301 tcagtcgacc gactttagcg aaatcttccg gcgtggcgtt gcggctgccc atcatcgtcg
     6361 tttctttttt atggaattcc ggatcggaaa actgcaactc acctttaaac aggccgacaa
     6421 ataccaccgt gccgccgtga cgaatcagat tcacggtgtt attcatcgca tgttgattac
     6481 ctgtcgcgtc gatcactttc tgcgccagcg aaccaccaaa ctgcgcccgt agctgcgcgt
     6541 caaaatcttc ggctgacggg tccagtaaag gtaattccag acgcgttgcc acatgttcac
     6601 ggcgcgccgg actggtatcc gccaccacca cctgtgcgcc atcggcttta gcgattgccg
     6661 ccgcgcccag accgattggc cccgccccga ccaccagcac ctgctcgccg ggagcaatgg
     6721 ctgcgcgacg caccgcatga gcgctaatag cgaaaggttc aatcaatgct gccgcctgcg
     6781 ggtcaatacc gtctgcgggc aaaatgttcg ccaccggcac gctcaaatac tcactaaaac
     6841 cgccatcctg atgcacgcca atgactgaaa ttttttcaca gcaattggta cgcccgcttt
     6901 tacacgccgg gcattgctga caggcaacat aagggatcac agcaacttgc tgaccatttt
     6961 taagatcagc aatcttttta cccagcccaa caatctcccc acatatttca tggcctaaaa
     7021 cacgtggata actaaaaaat ggttgatttc caccccaggc atgaatatcg gtaccgcaaa
     7081 tcccgacaga cttaattttt attaatgctt cattgtcacc cggaataggt atctcgcgtt
     7141 gtttccagac taattctttc ggctgctggc aaattaaaac attcatcgta gacatgattc
     7201 ggctctccat tttttgttgc cttagatatc aatgaaaatg aaaaatggca gcggaacgga
     7261 aactctgttt tgtgaaaaca cacgcataaa aatgttttaa atcgggtttt aatggactca
     7321 caaggaggaa aatatgagtc gttcacaaaa tttacgccac aatgtgatta accaggtcat
     7381 tgatgatatg gcgcggggtc atatcccttc cccacttcca tcgcaaagtg cgctggcgga
     7441 aatgtacaac atcagccgca ccacagtgcg tcatattctc agccatttac gcgaatgcgg
     7501 cgtcttgacg caggttggaa acgactatgt tattgcgcgt aaacctgacc acgatgacgg
     7561 tttcgcctgt actaccgctt caatgagtga acaaaacaaa gtgtttgagc aggctttttt
     7621 cacgatgata aaccagcgcc agttacgccc aggggaaact ttttctgaac tgcaactggc
     7681 tcgggccgcg ggtgtcagcc cggtggttgt aagagaatat cttttaaaat tcggccgtta
     7741 taatcttatt caaagcgaaa agcgcggcca gtggagcatg aagcaattcg atcaatccta
     7801 cgccgagcag ctgtttgagc tacgcgaaat gctcgaaaca cattcacttc agcactttct
     7861 caacctgcct gatcatgatc cccgctggct gcaggcgaaa accatgctcg aacgtcatcg
     7921 gctactgcgt gacaacatcg gcaatagctt tcgcatgttc tcgcaacttg acagagattt
     7981 tcactcctta ctgctttcag ctgccgacaa tatctttttt gatcaatcgc ttgagatcat
     8041 ctcagtcatc tttcattttc actatcaatg ggacgaaagc gatcttaaac agcgcaatat
     8101 cattgccgtc gatgagcata tgaccattct cagcgctctg atttgccgca gtgaccttga
     8161 tgccaccctt gcgctccgta accatttgaa ttcagctaaa caatcaatga ttcgatccat
     8221 taatgagaac acgcgttatg ctcattaagt acggattaaa aacagcaaac tattagcggg
     8281 tgcacggttt tatcaaaaga taaataccgg gaaattcccg cttacctatg ctcacaatca
     8341 gtcgactaaa aatgtttttg tgagcggtag taaagtccta aaactttaac ctgccgttaa
     8401 tgtcaacctt gcgtaatgca caattacgta aacaattaaa attataaata caggagtccg
     8461 gcgtggaaaa agaaaatatc accctcgatc cgcgttcttc atttactcca tcttcgtcgg
     8521 cagatattcc cgtgccacca gatggattag ttcaacgcag tacccgaatt aaacgcattc
     8581 aaaccaccgc catgttgtta ttattttttg cggcggtaat caattatctc gaccgcagtt
     8641 cgctgtcggt agcaaattta acgattcgtg aagaattggg attaagtgcc accgaaatcg
     8701 gcgctttgct ctccgtgttt tcactcgctt acgggattgc gcaacttcct tgcggcccac
     8761 tattggatcg taaaggccca cgcctgatgc tgggactggg gatgttcttc tggtcactgt
     8821 tccaggcaat gtctggcatg gtgcacaact ttacgcagtt cgtgttggtg cgtatcggta
     8881 tggggattgg tgaagcgccg atgaacccat gcggtgtaaa agtcattaac gactggttca
     8941 acatcaaaga gcgcggacgc ccgatgggct tcttcaacgc agcttctacc attggcgttg
     9001 ccgtaagccc accgattctg gcggcgatga tgctggtgat gggctggcgc gggatgttta
     9061 ttaccattgg tgtactgggg atttttctcg ccatcggctg gtatatgctc tatcgcaacc
     9121 gcgagcacgt agaactgact gccgttgaac aagcttatct caatgcaggt agcgtcaatg
     9181 cccgccgaga tccgctcagt tttgccgaat ggcgcagcct gttccgtaac cgtacaatgt
     9241 ggggaatgat gctcggattc agtggcatca actacactgc gtggctgtat ctggcctggc
     9301 ttcctggtta cctgcaaaca gcctataacc tggatttaaa aagcacaggg ttgatggcgg
     9361 ctatcccttt cctgtttggg gctgccggga tgctggtcaa cggttacgtt actgactggc
     9421 tggtcaaagg gggaatggct ccgattaaaa gccgtaagat ctgcattatt gccgggatgt
     9481 tctgttctgc cgcctttacg ctggtcgtac cacaagcgac aacatccatg acggcggttc
     9541 tgctgattgg catggcactg ttctgtattc actttgccgg aacatcctgc tggggcttga
     9601 tccacgtcgc agttgcttct cgcatgactg cgtcggtggg cagtatccag aactttgcca
     9661 gcttcatctg cgcctctttt gcgccgatca ttactggttt tattgttgat accacccact
     9721 cattccgtct ggcactaatc atctgcggtt gcgtcaccgc agcgggggca ctggcgtaca
     9781 tcttcctggt tcgtcagccg atcaacgacc cacgtaaaga ttaatctcct tatgcccgat
     9841 aacattttgc ttatcgggca ttttcatggc gattaaaatg tttcccagtt ctcctcgcta
     9901 tctgccacgg ccattttacg cggcgcagct ggcgtcacgg tttttaccac agccgatgtt
     9961 tcacgctgct gttgctgaat ccggaacact gccactgctt cggtcaggcg actggcctgc
    10021 tcttccagcg cggcggcggc ggcggcagat tcttccacca gcgcggcgtt ctgttgagtt
    10081 acccggtcca tctcagcaac cgctaagcca acctgatcga taccacggct ctgctcatca
    10141 gaagcagaag ctatttcgcc cataatgtcc gtcacgcggg tcacagcact gacaatctcc
    10201 gccattgttt ccccggcgct ttcgaccagt gtagagccaa catccacttt ccccaccgag
    10261 tcttcaataa ggcttttaat ttcacgagcc gcctgagcgc tacgctgggc cagattacgc
    10321 acttctcccg caaccaccgc aaaaccgcgc ccttgctcac ccgcacgcgc tgcttcaacc
    10381 gccgcgttca aagccagaat attggtctgg aaggcaatgc cgtcaattac gctgataata
    10441 tcggcgattt tctgcgaact ggtggagata tcgcgcatag tctgcaccac gttatctacc
    10501 actttgccgc cccgttgcgc cgtttcagaa gcacttaatg ccagatggct ggcctggcgc
    10561 gcattctcgg cgttctgttt caccgttgcg gtcagttgct ccatgctggc tgccgtctct
    10621 tccagcgaag cggcctgttg ctcggtgcgc gaagagagat cgttattgcc ggtagcgatt
    10681 tcgctggcac cgctatagat ggcattggcc ccgttgcgca catcaccgac ggtacgcatc
    10741 agctctccct gcatatggcg caaactctct gccagttgcc ccatctcatt agagccatcc
    10801 acctcaatcg gtttcaccag atcgccgcct gcaatatgac ggatgctgtc aatcaggcga
    10861 ttcattggcg ctaccagcga ggctttaata ccgaaccaga cggcgaagat gaccgccagt
    10921 acgacgatca tcacgcccac cagaatccac atcgcctggc tgtaggaggc attgttatcg
    10981 ctgacggcga tatcatagag ccgatcgttt tgctccatgt aagccacata ctgcttctcg
    11041 aaaccgtctt gatatccctg ggtcggctga tcaaagaact cgttgatttt gcctgcacct
    11101 aacagttgga tcagctccgc cagcgcattg tgataaatat cgtaattacg tttgatctct
    11161 gccgctgcgg cggtgctctg acgcgggtca cgcggcaacg cttcgtaatc cgcccagttt
    11221 ttttccgcct gtttcagcga aatactggca ctctgcatca gctcagcaac ggttgaaccg
    11281 ctaccaatat tattctgatc catcatgtag cggatacccg cgcggttgag ggtgttacgc
    11341 gtctgcaaca acgcgaccca gctgccattc agcgtggatt gctgctggcg aatggtttgt
    11401 aaaacagtga aattttcttt gtcattcttt aaggcattaa agaacagacc gcctgatgtc
    11461 agttgtaaaa ggccaaaaac ggccaaaacc agcagtaagc tggtcacgat cttgatacgt
    11521 tttaacatgg tttctctttc ctgtggaccg atgcaagatt ttcggcctgg aaagcaaaaa
    11581 ctttatgaaa aggtgtaacc cgccatccat ggaaaaacca ggcgctggtt atcagatact
    11641 aaaaagttat tcattatcgc ccgggatggc ttcttgtcgg gaattgccca gagcaataaa
    11701 ttcctctaac agatgggtta actgttgcat tttctcggca gtaaactgag cttcaatctg
    11761 ccgataggct tcttcaatct gtgtctgagc gcggttatac agcgcctgcc cctctttggt
    11821 tagcgagata tagagcttcc gctgatcatt gatcggcttt aatcgcaaca ctaaaccgtc
    11881 gcgttccatc cgcgtaagga tcccggtcag gcttgggcgc aaaatgcagg cgcgatacgc
    11941 cagatcgtga aaatccattg atgggctttc cgccagaata cgcacgatgc gccactgttg
    12001 ctcagtcaaa ttatgccgtt taacgattgg gcgaaaataa ctcatcgccg cttctcgcgc
    12061 ctggagcaac gcaatggtta gagagtcgtg cattatcttt cccctgaaaa attcaaaatc
    12121 attaatatag aaacagttaa tcatcacgtt accgggactg cgctgcccct gcaattcttc
    12181 attttatcaa aattgagcat attttgttaa cttgctgata tttaatattt tattatcttt
    12241 aaatgtaaat agtttgttaa ttagatcaca tttacatcac ttacttttgc caaactacgc
    12301 aatcgggaat aaaagtaatc attaacatat taatgattaa aatgatcgaa tccgaggagt
    12361 ggtaatgaaa ggcactatct tcgccgtagc gttgaaccat cgcagccaac ttgatgcatg
    12421 gcaggaagcg ttccagcaat ccccctacaa agccccgcct aaaactgcgg tctggtttat
    12481 taaaccgcgc aatacggtga ttggttgcgg tgaaccgatt ccctttccac agggtgaaaa
    12541 ggtactgagc ggtgcgactg ttgcgctgat tgtgggaaaa acggcgacga aagtacgtga
    12601 agaagatgcg gcagagtaca tcgccggata tgcgctggct aacgacgtca gcctgccgga
    12661 agagagcttt taccgcccgg caatcaaagc aaaatgtcgt gatggattct gccccattgg
    12721 cgaaaccgtg gctctcagca atgtcgataa tctgaccatc tataccgaga tcaacgggcg
    12781 tcctgccgat cactggaata ccgccgattt acaacgtaac gccgcgcagt tgctgagcgc
    12841 cctgagcgaa tttgccacgc tgaatccagg cgacgccatt ctgctcggca cgccacaggc
    12901 gcgcgtggaa atacagccag gcgatcgcgt tcgtgttctc gcagaaggtt tcccgccgct
    12961 ggaaaatccg gtagtggacg aacgtgaagt gaccacgcgc aagagcttcc caacgcagcc
    13021 acacccgcac ggcacgctgt ttgccctcgg cctgaactac gccgaccacg ccagcgaact
    13081 ggaatttaag ccaccggaag aaccgctggt gttcctgaaa gcgccaaata ccctcactgg
    13141 cgataaccag acctccgtgc gtccaaacaa tattgaatac atgcactatg aagcggagct
    13201 ggtggtagtt attggcaaac aggcgcgtaa cgtcagcgaa gccgatgcca tggattatgt
    13261 cgcgggctac accgtgtgta acgactacgc cattcgcgac tatctggaaa actactaccg
    13321 ccctaacctg cgggtcaaaa gccgcgacgg actgacgccg atgctttcaa ccatcgtgcc
    13381 gaaagaggcg atcccggacc cgcataatct gacccttcgc accttcgtca acggcgagtt
    13441 acgccagcaa ggcaccaccg ccgatctgat cttcagcgtg cccttcctga tcgcctactt
    13501 aagcgaattt atgaccctga atccgggcga catgatcgcc accggcacac caaaaggctt
    13561 atctgacgtg gtgcctggcg atgaagtggt ggtggaagta gaaggcgtgg gccgcctggt
    13621 gaaccgaatt gtgagtgagg aaacagcgaa atgaaaaaag taaatcattg gatcaacggt
    13681 aaaaatgttg caggtaacga ctacttccag actaccaatc cggcaacggg tgaagtgctg
    13741 gcggatgtgg cctccggcgg tgaagcggag atcaatcagg cggtagcggc agcgaaagag
    13801 gcgttcccga aatgggccaa tctgccgatg aaagagcgcg cgcgcctgat gcgccgtctg
    13861 ggcgatctga tcgaccagaa cgtgccggag atcgccgcga tggaaaccgc ggacacgggc
    13921 ctgccgatcc atcagaccaa aaatgtgttg atcccacgcg cttctcacaa ctttgaattt
    13981 ttcgcggaag tctgccagca gatgaacggc aagacttatc cggtcgacga caagatgctc
    14041 aactacacgc tggtgcagcc ggtaggcgtt tgtgcactgg tgtcaccgtg gaacgtgccg
    14101 tttatgaccg ccacctggaa ggtcgcgccg tgtctggcgc tgggcaatac cgcggtactg
    14161 aaaatgtcgg aactctcccc gctgaccgcc gaccgcctgg gtgagctggc gctggaagct
    14221 ggtattccgg cgggcgtgct gaacgtggta cagggctacg gcgcaaccgc aggcgatgcg
    14281 ctggtccgtc atcatgacgt gcgtgccgtg tcgttcaccg gcggtacggc gaccgggcgc
    14341 aacatcatga aaaacgccgg gctgaaaaaa tactccatgg aactgggcgg taaatcgccg
    14401 gtgctgattt ttgaagatgc tgatattgaa cgcgcgctgg acgccgccct gttcaccatc
    14461 ttctcgatca acggcgaacg ctgcaccgcc ggttcgcgca tctttattca gcaaagcatc
    14521 tacccggaat tcgttaaacg ctttgccgaa cgcgccaacc gtctgcgcgt gggcgatccg
    14581 aacgatccga atacccaggt tggcgcgctt atcagccagc agcactggga aaaagtctcc
    14641 ggctatatcc gtctcggcat tgaagaaggc gcaaccctgc tggcgggcgg cccggataaa
    14701 ccgtccgacc tgcctgcgca cctgaaaggc ggcaacttcc tgcgcccaac cgtgctggca
    14761 gacgttgata accgtatgcg cgtcgcccag gaagatattt tcgggccggt cgcctgcctg
    14821 ctgccgttta aagacgaagc tgaaggctta cgcctagcaa acgacgtgga gtacggcctc
    14881 gcgtcgtaca tctggactca ggatgtcagc aaagtgctgc gtctggcgcg tggcattgaa
    14941 gctggcatgg tgttcgtcaa cacccagaac gtgcgtgatt tgcgccagcc gtttggcggc
    15001 gtaaaagcct ccggcaccgg gcgtgaaggc ggtgagtaca gcttcgaagt gttcgcggaa
    15061 atgaagaacg tctgcatttc catgggcgac catccaattc cgaaatgggg agtctgatat
    15121 gggtaagtta gcgttagccg ccaagatcac gcacgtaccg tcgatgtatc tctctgaact
    15181 gccagggaaa aaccacggtt gccgccaggg cgcgatcgac gggcataaag agatcagcaa
    15241 gcgttgccgg gaaatgggcg tcgataccat tatcgttttc gatacccact ggctggtcaa
    15301 cagtgcttat cacatcaact gtgcagacca ttttgaaggc atctacacca gcaacgagct
    15361 gccgcatttt atccgcgata tgacctacaa ctacgatggc aacccggagt tggggcagct
    15421 tattgccgat gaagccttaa agctcggcgt gcgggcaaaa gcgcacaaca ttcccagtct
    15481 gaaactggaa tacggcacgc tggtaccgat gcgctacatg aatgaagaca agcacttcaa
    15541 agtggtctcc atttcggctt tctgcacggt tcacgatttt gctgacagcc gcaagctggg
    15601 cgaagcgatt ctgaaagcga tcgaacagta cgacggcacc gtggcggtcc ttgccagcgg
    15661 ttcgttatcg caccgcttta ttgacgatca gcgtgcagaa gaagggatga acagctacac
    15721 ccgcgagttc gaccgccaga tggacgagcg cgtggtgaag ttgtggcgcg aaggccagtt
    15781 caaagagttc tgcaatatgc tgccggagta cgccgactac tgctacggcg aaggcaatat
    15841 gcacgacacg gtgatgctgc tggggatgct cggctgggat aaatacgacg gcaaggtgga
    15901 gtttatcacc gagctattcc caagctctgg caccggtcag gttaacgctg ttttcccgct
    15961 tcccgcgtaa ggaaccacca tgccgcactt tatcgtggaa tgtactgaaa atattcgtga
    16021 agaagcccgc ttacctgagc tgtttgccag tgttaacact gcactggcgg caacggggat
    16081 ttttccgctt gggggaatac gtagccgggc gcactggatc gatacctggc aaatggctga
    16141 tggcaagcac gattacgcct ttgtgcatat gacgctaaaa atcggcagcg ggcgcagcct
    16201 ggaaagccgt caggaagtgg gcgaaatgct gtttgatctg attaaaacgc acttcgcctc
    16261 actgatggag agccgttacc tggcgctttc atttgagatt gcagagctgc acccgacgct
    16321 gaattttaaa caaaacaacg tgcacgcgtt gtttaaataa atttgcacca gcttccaggc
    16381 cggatgataa ggcgcagccg catccggcaa tgccacagga tatcgctatg ttcgataaac
    16441 acacccacac cctgatcgcc cagcgtctgg atcaggcaga aaaacagcgc gaacagatcc
    16501 gcgcgatctc gctggattac ccggagatca ccatcgaaga cgcttacgcg gtgcagcgtg
    16561 aatgggttcg actgaaaatc gccgaaggtc gcacgctgaa aggccacaaa atcggcctga
    16621 cctcgaaagc gatgcaggcc agctcacaaa tcagcgagcc ggattacggt gccctgctgg
    16681 atgacatgtt cttccacgat ggcagcgata tcccgaccga tcgctttatc gtgccgcgca
    16741 ttgaagtgga gctggctttc gtgctggcaa aaccgctgcg tgggccaaac tgcacgctgt
    16801 tcgacgtcta caacgccacg gactatgtga tcccggcgct ggagctgatc gacgctcgct
    16861 gccacaacat cgatccggaa acccagcgcc cgcgcaaagt gttcgacacc atttccgata
    16921 acgccgccaa cgccggggtg atcctcggcg gtcgtccgat caaaccagac gaactggatc
    16981 tgcgttggat ctccgccctg atgtatcgca atggcgtgat tgaagaaacc ggcgtcgccg
    17041 ctggtgtgct gaatcatccg gcaaacggcg tggcctggct ggcgaacaaa ctcgcaccgt
    17101 atgacgtaca actggaagcc gggcaaatca ttctcggcgg ttcgttcacc cgcccggttc
    17161 cggcgcgtaa gggcgacacc ttccacgtcg attacggcaa catgggctcc attagctgcc
    17221 gctttgttta aggagagaac gatggaaaac agttttaaag cggcgctgaa agcaggccgt
    17281 ccgcagattg gattatggct ggggctgagc agcagctaca gcgcggagtt actggccgga
    17341 gcaggattcg actggttgtt gatcgacggt gagcacgcgc cgaataacgt gcaaaccgtg
    17401 ctcacccagc tacaggcgat tgcgccctac cccagccagc cggtagtacg tccgtcgtgg
    17461 aacgatccgg tgcaaatcaa acaactgctg gacgtcggca cacaaacctt gctggtgccg
    17521 atggtacaaa acgctgacga agcccgtgaa gcggtacgcg ccacccgtta tccccccgcc
    17581 ggtattcgcg gtgtgggcag tgcgctggcc cgagcctcgc gctggaatcg tattcctgat
    17641 tacctgcaaa aagccaacga tcaaatgtgc gtgctggtgc agatcgaaac gcgtgaggca
    17701 atgaagaact taccgcagat tctggacgtg gaaggggtcg acggcgtgtt tatcggcccg
    17761 gcggatctga gcgccgatat gggttatgcc ggtaatccac agcacccgga agtacaggcc
    17821 gcgattgagc aggcgatcgt gcagatccgc gaagcgggca aagcgccggg gatcctgatc
    17881 gccaatgagc aactggcaaa acgctatctg gaactgggcg cgctgtttgt cgccgtcggc
    17941 gttgacacca ccctgctcgc ccgcgccgct gaagcgctgg cagcacggtt tggcgcacag
    18001 gccacgaccg ttaagcccgg cgtgtattaa tgccggatgc gctgcactta tccggcctac
    18061 actcgcacca aaccgtaggc cggataaggc gtttacgccg catccggcaa aaagccgtac
    18121 caaatcgcgg accggataag gcgtttgtgc gcatccgcca taaaccttgt cgtaccctac
    18181 aaaaatccca ttagaggaag aaaaatgagc gacacctcac ctgccatacc ggagagtatc
    18241 gatccggcga atcagcataa agcgctgact gccggacaac aggcggttat taagaagctg
    18301 tttcgccgcc tgatcgtctt tctgttcgtg ctgtttatct tctcgttcct tgatcgcatc
    18361 aacatcggct ttgccggact cacgatggga cgcgacctcg gtctgagcgc caccatgttt
    18421 ggcctcgcta ccaccctgtt ctatgccgct tatgtcatct tcggcattcc cagcaacatt
    18481 atgctgagta ttgtcggtgc gcggcgctgg atcgccacca tcatggtgct ctggggcatc
    18541 gcctctactg ccaccatgtt tgccactggc cccaccagct tgtacgtact gcgtatactg
    18601 gttggcatta ccgaagccgg ctttctgcct ggtattctgc tgtatttaac cttctggttc
    18661 ccggcctatt tccgcgcccg tgccaacgcc ttgtttatgg ttgcaatgcc ggtaacgaca
    18721 gcgttgggat cgctcgtttc cggctacatt ttgtcgctgg atggcgtaat ggcattaaaa
    18781 ggctggcagt ggctgttttt gctggaaggc ttcccgtcgg tattactcgg cgtcatggtg
    18841 tggttctggc ttgatgactc accggacaaa gctaagtggc tgacgaaaga agacaaaaaa
    18901 tgcctgcaag agatgatgga taacgatcgt ctgacgctgg ttcagccaga gggagccatc
    18961 agccaccacg ccatgcaaca acgcagcatg tggcgggaga tcttcactcc ggtggtgatg
    19021 atgtataccc tggcgtattt ctgcctgacc aacacactta gtgcgatcag catctggaca
    19081 ccgcagatcc tgcaaagctt taatcagggc agcagtaata tcaccatcgg cctgctggcc
    19141 gccgtaccgc agatttgtac cattctcggg atgatctact ggagccgtca ctcagatcgc
    19201 cgccaggaac gaaggcatca caccgccctt ccttatttgt tcgctgccgc gggttggtta
    19261 ctggcttcgg caactgacca caacatgatc cagatgctgg ggatcattat ggcttcgacc
    19321 ggatcattca gcgcaatggc gattttctgg acaacacctg atcagtccat cagcctgcgg
    19381 gcacgagcga tcggtattgc ggtgatcaac gccactggca acattggctc agcgttaagt
    19441 ccgtttatga tcggctggtt gaaagatctg accggcagct ttaacagtgg attgtggttt
    19501 gttgccgcgc tgctggtgat tggtgcgggg attatctggg caattccaat gcagtcctcc
    19561 cgtccgcgag cgaccccgta aggaacgacg atgtgtgacc gtcagattgc caatattgat
    19621 atcagcaaag agtacgatga aagcctgggc acggacgatg tgcattatca gtccttcgcc
    19681 cgcatggcgg cattttttgg ccgccatatg ctgccacatc gccacgaaca gtactttcag
    19741 atgcatttcc tcaatagcgg acagattgag ctacagcttg acgatcatcg ctactcggtg
    19801 gaagcgcccc tgtttgtcct gacgccgccg tcagtacctc atgcgtttat tacggagtct
    19861 gacgccgacg gtcatgtgtt gacggtacgg gaagatctga tctggcccct gctggaagtt
    19921 ctttatccag gcactcggga aaccttcggc ctgccgggga tttgcctgtc actggcagat
    19981 aaacccgacg aactggcggc gctggaacac tattggcaac tgatagagcg ggaatcggta
    20041 gaacaactgc ctggacggga acacaccctg acgttactgg cacaggcagt gttcacccta
    20101 ctgctgcgta acgcaaaact cgacgaccat gccgccagcg gaatgcgcgg agaattaaaa
    20161 ctgttccagc gttttcatat gctgattgaa agccattttc atcagcactg gacagtaccg
    20221 gattacgcta acgaactgca tatcaccgaa tcacgcctca cggacatctg ccgccgcttt
    20281 gccaaccgtc cgccaaaacg gttgattttc gacaggcagc tgcgagaaac caagcggctg
    20341 ctgctgtttt ctgataacgc cgtgaacaat attgcctggc aactcggttt taaggatcca
    20401 gcttattttg cgcgcttttt taatcgctta gtcggttgct cgcccagtgc ttatcgtgcc
    20461 aaaaaagtac ctgtgacgtg aaaatccctt agccttaacg ggaaaccagg caccacctgc
    20521 tattcccctt tctccgcctg gagcaagggg atttagtaac gtcaaaaatc gctaaaagcg
    20581 acctcgatca caaaaccgta cccgacctga aaagtaccag ccattgtccc aaaagtgtct
    20641 tcccgtaatc gcccctgcaa gcttcaattg aataacaaaa acaacacaca aaattaacaa
    20701 tcgtattccg attaatactg tagaggtcga catgaaacca gaagatttcc gcgccagtac
    20761 ccaacgtccg ttcaccgggg aagagtatct gaaaagcctg caggatggtc gcgagatcta
    20821 tatctatggc gagcgagtga aagacgtcac tactcatccg gcatttcgta atgcggcagc
    20881 gtctgttgcc caactgtacg acgcgctgca caaaccggag atgcaggact ctctgtgctg
    20941 gaacaccgac accggcagcg gcggctatac ccataaattc ttccgcgtgg cgaaaagtgc
    21001 cgacgacctg cgccagcaac gtgacgccat cgctgagtgg tcacgcctga gctatggctg
    21061 gatgggccgt accccagact acaaagctgc tttcggttgc gcactgggcg cgaatccggg
    21121 cttttacggt cagttcgagc agaacgcccg taactggtac acccgtattc aggaaactgg
    21181 cctctacttt aaccacgcga ttgttaaccc accgatcgat cgtcatttgc cgaccgataa
    21241 agtgaaagac gtttacatca agctggaaaa agagactgac gccgggatta tcgtcagcgg
    21301 tgcgaaagtg gttgccacca actcggcgct gactcactac aacatgattg gcttcggctc
    21361 ggcacaagtg atgggcgaaa acccggactt cgcactgatg ttcgttgcgc caatggatgc
    21421 cgatggcgtg aaattaatct cccgcgcctc ttatgagatg gtcgcgggtg ctaccggctc
    21481 gccgtacgac tacccgctct ccagccgctt tgatgagaat gatgcgattc tggtgatgga
    21541 taacgtgctg atcccatggg aaaacgtact gatctaccgc gattttgatc gctgccgtcg
    21601 ctggacgatg gaaggcggtt tcgcccgtat gtatcctctg caagcctgtg tgcgcctggc
    21661 agtgaaactc gacttcatta cggcactgct gaaaaaatca ctcgaatgta ccggcaccct
    21721 ggagttccgt ggtgtgcagg ccgatctcgg tgaagtggtg gcgtggcgca acaccttctg
    21781 ggcattgagt gactcgatgt gttctgaagc gacgccgtgg gtcaacgggg cttatttacc
    21841 ggatcatgcc gcactacaaa cctatcgcgt actggcacca atggcctacg cgaagatcaa
    21901 aaacattatc gaacgcaacg ttaccagtgg cctgatctat ctcccttcca gtgcccgtga
    21961 cctgaacaat ccgcagatcg accagtatct ggcgaagtat gtgcgcggtt cgaacgggat
    22021 ggatcacgtc cagcgcatca agatcctcaa actgatgtgg gatgccattg gcagcgagtt
    22081 tggtggtcgt cacgaactgt atgaaatcaa ctactccggt agccaggatg agattcgcct
    22141 gcagtgtctg cgccaggcac aaagctccgg caatatggac aagatgatgg cgatggttga
    22201 tcgctgcctg tcggaatacg accagaacgg ctggactgtg cctcacctgc acaacaacga
    22261 cgatatcaac atgctggata agctgctgaa ataacgcagc aggaggttaa gatgcaatta
    22321 gatgaacaac gcctgcgctt tcgtgacgcg atggccagcc tgtcggcagc ggtaaatatt
    22381 atcaccaccg agggcgacgc cggacaatgc gggattacgg caacggctgt ctgctcggtc
    22441 acggatacac caccgtcgct gatggtgtgc attaacgcca acagtgcgat gaacccggtt
    22501 tttcagggca acggcaagtt gtgcgtcaac gtcctcaacc atgagcagga actgatggca
    22561 cgccacttcg cgggcatgac aggcatggcg atggaagagc gttttagcct ctcatgctgg
    22621 caaaaaggtc cgctggcgca gccggtgctg aaaggttcgc tggccagtct tgaaggtgag
    22681 atccgcgatg tgcaggcaat tggcacacat ctggtgtatc tggtggagat taaaaacatc
    22741 atcctcagtg cagaaggtca cggacttatc tactttaaac gccgtttcca tccggtgatg
    22801 ctggaaatgg aagctgcgat ttaagctcca gccccttcac gcattcgtcg aaggggcatt
    22861 tttattgtga tctcaatcac atatccaccc gatgaataat tcttgcccaa ccgctcaccg
    22921 ccattcctga ccgcttacgt ccctaaaata accactcagt tatttacctt actttacgcg
    22981 cgcgtaactc tggcaacatc actgcaggat agcggtcaat ttacctcctc aaacgcaacg
    23041 caaacctaga acggcttcgg cctatgatta acaaattact ctatgaattt tggtttgcag
    23101 gaaggcagca agtgagtgaa tccccgggag cttacaacag taagtgacag gggtgaacga
    23161 acgcaactgc cgcacctgta agctaaaaga cgacgagtaa aatgccaggt tttactatgg
    23221 atacgaaaaa actattcaag cacataccct gggtgattct cggaatcatc ggtgcattct
    23281 gcctcgcggt agttgcatta cgtcgggggg agcacgtcag cgccctgtgg atcgtggtcg
    23341 cctctgtgtc ggtgtatctg gtggcgtatc gctactacag tctgtacatc gcccagaagg
    23401 tgatgaaact cgaccccacg cgcgcgacgc ctgcggttat taacaacgac ggtctgaact
    23461 acgttccgac caaccgttac gtgttgtttg gtcaccactt cgccgctatc gccggtgctg
    23521 gtccgctggt cggtccggtt ctcgccgcac agatgggcta cctgcctggc acgctgtggc
    23581 tgctggcggg ggtagtactg gccggtgcgg ttcaggactt tatggtgctg tttatctcct
    23641 ctcgccgtaa cggcgcatct ctcggtgaga tgataaaaga agagatgggg ccagttccgg
    23701 ggactatcgc gctgtttggc tgtttcttaa tcatgatcat catcctcgcc gttctggcgc
    23761 tgattgtggt taaagccctg gccgaaagtc cgtggggtgt cttcaccgtt tgctcaaccg
    23821 taccgattgc gctgtttatg ggtatctaca tgcgctttat ccgtccgggg cgtgtgggtg
    23881 aagtctctgt cattggtatc gtgctgctgg ttgcctctat ctacttcggt ggcgtgattg
    23941 ctcacgatcc gtactggggt ccggcactga cctttaaaga caccaccatt accttcgcgc
    24001 tgattggcta tgcgtttgtt tccgcactgc tgccggtgtg gctgatcctc gcaccgcgcg
    24061 actatctggc aaccttcctg aaaatcggcg ttatcgtcgg cctggcgctg ggtatcgtgg
    24121 tgctgaaccc ggaactgaaa atgcctgcga tgacccagta cattgacggt actggcccgc
    24181 tgtggaaagg cgctctgttc ccgttcctgt tcatcaccat cgcctgtggt gcggtatctg
    24241 gcttccacgc gctgatctct tccggtacga cgccaaaact gctggctaac gaaaccgacg
    24301 cgcgtttcat tggctacggc gcaatgctga tggagtcctt cgtggcgatt atggcgctgg
    24361 ttgcagcgtc catcatcgaa ccgggtcttt acttcgcgat gaataccccg cctgctggcc
    24421 ttggcatcac catgcctaac ctgcatgaaa tgggtggcga gaacgcgccg atcatcatgg
    24481 cgcagctgaa agacgttacc gcacacgcgg cagcgaccgt cagctcctgg ggcttcgtga
    24541 tttcgccaga gcagatcctg caaaccgcga aagacattgg tgagccttct gtcctgaacc
    24601 gtgcaggtgg tgcgccaacg ctggcggtag gtatcgctca cgtgttccac aaagtgctgc
    24661 cgatggctga catgggcttc tggtatcact tcggtattct gttcgaagcc ctgttcatcc
    24721 tgaccgcgct ggatgcgggt actcgttctg gccgctttat gctgcaagac ctgctgggta
    24781 acttcatccc gttcctgaaa aaaaccgact ctctggttgc cggtatcatc ggtactgcgg
    24841 gctgtgtggg tctgtggggc tacctgctgt atcagggcgt ggtcgatccg ctgggcggcg
    24901 ttaagagcct gtggccgctg ttcggtattt ccaaccagat gctggcagcc gtagcgctgg
    24961 tactgggcac cgttgtgctg attaagatga agcgcaccca atacatctgg gtaactgttg
    25021 ttccggctgt atggctgctt atctgcacca cctgggcgct gggtctgaaa ctgttcagca
    25081 ccaacccgca gatggaaggc ttcttctaca tggcaagcca gtacaaagag aagattgcta
    25141 acggtactga cctgacggcg cagcagattg ccaacatgaa ccacatcgtt gtgaacaact
    25201 acaccaacgc aggcctgagt attctgttcc tgattgtggt gtacagcatc atcttctacg
    25261 gtttcaaaac ctggctgaat gtgcgtaaca gcgacaaacg tactgacaaa gaaacaccgt
    25321 acgttccaat cccggaaggc ggcgtgaaga tctcttcgca ccactaaccg tatttagccc
    25381 cgcttcggcg gggctttgtt cttatcagag tgaactatgt ttggtaactt aggacaggca
    25441 aaaaaatatc tcggccaggc ggcgaagatg ttgattggca ttccagacta cgacaactac
    25501 gtcgagcata tgaagaccaa ccatcccgac aagccgtaca tgagctatga agaattcttc
    25561 cgcgaacgcc agaacgcacg ctacggcggc gatggtaaag gcggtatgcg ctgttgttaa
    25621 tggagagagc atgaacccga ttgcagttac cctactcacc ggttttttag gcgcgggcaa
    25681 aaccaccctg ctgcgccata ttcttaatga acaacacggc tacaagattg ccgtgattga
    25741 aaacgaattc ggcgaagtct ccgttgatga tcaattgatt ggtgatcgcg caacgcagat
    25801 caaaacgctg accaacggct gcatctgctg ttcgcgctcc aacgagctgg aggacgcgct
    25861 actcgacctg ctggataatc tcgacaaggg caatattcag ttcgatcgtc tggtcattga
    25921 atgcaccggc atggccgatc ctggcccgat tattcaaacc tttttctccc atgagatttt
    25981 atgccagcgc tacctgctgg acggcgtgat tgcgctggtg gatgcggtac atgccgatga
    26041 gcagatgaac cagttcacca tcgcccagtc ccaggtgggc tacgccgacc gtattctgct
    26101 gactaaaacc gacgtcgcag gcgaagcaga aaaactgcgt gaacgtctgg cacgcatcaa
    26161 cgcccgtgca ccggtctaca ccgtcaccca cggcgacatc gacctgggtc tgctgttcaa
    26221 caccaacggt tttatgctgg aagaaaacgt cgtcagcacc aaaccgcgtt tccattttat
    26281 cgcggacaaa caaaacgata tttcgtcgat tgtggtggaa ctggattacc cggtagatat
    26341 cagcgaagtt tcccgggtga tggaaaacct gctgctggaa tcggcggata aactgctgcg
    26401 ttacaaaggg atgctgtgga ttgacggcga acctaaccgc ctgctgttcc agggcgtcca
    26461 gcgcctctac agcgccgact gggacaggcc atggggcgat gaaacaccgc atagcacgat
    26521 ggtgtttatc gggattcagt tgccggagga tgagattcgg gcggcgtttg cgggactcaa
    26581 aaagtaacca gccctccaac atcacaatac ctgagacttt atcaccctca tattcctcca
    26641 gaatatgagg gctttcgggc ttaattatct ctgaacgtgc aacacttcct gcgactcctt
    26701 atgcgcctcc acatcctgct tcgttaacca gagcgactta cggccaaaat tttcgtacat
    26761 tttcagctga tcttcatagt gcttatcaac tgttccatcg ggagcaataa acccactctg
    26821 accgggtgcg accacatccc aggcaagcac aggacgatcg cttgtcgttg gtgagaaaac
    26881 aatcatatcg ttttctgttc cacggttttg atactccgcc tgatgacgcg tttcttccgc
    26941 tgcggcctgc ggtacaccaa agaaattatt tgcccggaac gttaaggcca ttgcaggtgt
    27001 tttccagtta ctcacattat tgccatagcg tttggaaaga gtctcccagg tatcttccag
    27061 cgcagccaac acaacctcct gctgtggttt cccagcaaac agatcaaccg cctgtgggat
    27121 tggtgatttg tctccctgca ccgcctcata caaaattttt gctccaacac ttatattcag
    27181 cgaaccagtt gggccgtcct gggttgtttc gtagccactg gcgctgtacc acttatcaaa
    27241 tggcataggt acggcagcca ctacggtacg cttcaacata ctggtcagcc aaacgttcag
    27301 gatggcagag cctggctgct gccaggtttt accatcatca ttaagcaaat tgatgccatc
    27361 ccaacgtgtt aatgtttcta ccaactgacg acgcggatcg ctctgtgtca aaccagatgt
    27421 cgctgcttgc agagtaggta aaaaaagcct caggttaaga tcctgacgac tggtttggcg
    27481 aataacatcc catgcctgat cagcagttaa gcgtggcttt tgctcaagca gtcggtcgat
    27541 ctccgtaacg cgatctgcac caccccacaa aaaggcaaac agatctgaag cgggataatc
    27601 tttttgggga gaattgttcc agttagcaat atatcccgac tgggggttat acaccttagg
    27661 gttcatttca aaaggcaata gccctttcca gtcccatttt cccgtaccag gaacgggtaa
    27721 tcgcggatca tggcctgatt gacgatctgg ataagcacca gtatgaacat aaccaatatt
    27781 gccgtttaca tcagcatagt accagttgat ggtcagtgct tgtttcgctg cctgctgtgt
    27841 ccactcctgc caatttttgg ccttcatctg atgagtccag gccagcaaag acgccacctc
    27901 tttaccatcc catgcgcggg atttagcgta agccgtttgt gtcgtctggt cagtttggag
    27961 aatgttgcca tgcaccgtac gccagacagt aaaggtctct gcctgaccat ttttcaccgt
    28021 aatggtttcc tcacggctta acattttcac ccacttacca ttatgcaagt agtagcctgg
    28081 tttctctgcc gacagccgtt cagcaaaaat atcgacatca tcgccgaaac ctgccgttga
    28141 tccccaggaa atcacaccat tatgaccaaa aaccagccca ggataggcaa atggtgtatt
    28201 gccagtgaca tcataaccag caccgtgcag accaatacca taagtatacg caggcgcata
    28261 ccagccaaac tgcggaccat ttaccatgat tgctttcgca tcctgagctt tgcttttgcc
    28321 aatcacccac atattgctgg tcgttggata ccccgccaga ccattggcac caccctgtgc
    28381 aaattgtgca gcaatagttt cccggttctt ccctgctgtt aacgccagca gtgcgccatc
    28441 cgcccctttt gctggtcggt caagcattgg tgcaggtaaa tcgtagcgtg gcaacagagc
    28501 tgctgtttgc gagttttgct gattaaattt aagtgggtag ttactctctt gtacggcaat
    28561 agtggttggc gctgatgggt ttaccagcca tttcaactga ttaaataccg ccatgccttg
    28621 tgatacacca tatttatctt ttaaagccgt tagcagtgcc agattatcaa tttcgctagt
    28681 gctatcagag aagcggtttg ccatggtgcc cacaaatatc atcgcgacat caaacggttc
    28741 ccagcgctta ggagtaaagc caaatgtatt aaactgtttt ggtaagagcg tctctggatt
    28801 ggtatttacc ttatcaatcc aggcattcat tccatcagcg tagccttgca gaatggacat
    28861 atcctctggg gaaagggcag caatttgcgc ccggatagca tccggccagt agttacgacg
    28921 gatatcttta tcaaatttca caaaatcttt gccaagcact tccgcgacag tcccttgagt
    28981 actgcgacgt gccatttcca tctgaaaaag gcgatcttgt gctactacat agccatagcc
    29041 ataaaatagg tgccatgtat cattggcata aatatgcggc atgccgtatt catcgcgaac
    29101 aatctttatc tcacttgacg actgctcagc cagtgcaggt aagctccaat aatacatcag
    29161 ggaagcagta acacagttca cgatcatacg atttctattt ttcattgtat cctctggcag
    29221 gtgtataatt agcgaattga tactagcaac gaagcttcgg ttatctattt gcagagaatt
    29281 gtttcattat gaactgtgag cgcgataact aatttttgat acaaaaattt cattaatgtg
    29341 atctaaaacg aatcgcttta ttcaccgctg atgattttgt tctattcctt tttgtgacag
    29401 atcccccgga cgcatagatg atgaccccca gagagacagc tcaggtcatt atctgtatgt
    29461 acatcagaaa aatatgttgt aaacgacttt tatggttgga ttttgtagca attttgaatg
    29521 cagacaggca gcatttgcca acacgccgcc catagttcac tccacatcct ccctgcgtaa
    29581 cccaatatcc ctcaaccgtt catcactcat ctgctgtaac actcgtcggg tctgcatttg
    29641 ccgccgccaa cgcctcaccg catgccagag ttgcacaagt ctgataaacg gcgttttggc
    29701 tttgttttcg tgaaattcca tctgctgcct cctcatcttt cagaggcttc atcatccgcc
    29761 ttctggcagt tcacaataca gatgcaaata ttagttttat ttaacataca gacaggttaa
    29821 aacagcatct gcatccggtt ttttagcctc atctgtaccg gtttttcttt ctgtatgggc
    29881 caaataagga atacagcatg acgcgttatc aacatctggc gactctactt gccgagcgga
    29941 ttgagcaagg gctgtatcgt cacggggaga aattgccgtc ggtgcgcagc ttaagtcagg
    30001 agcacggcgt cagcatcagc accgtgcaac aggcgtatca gacgctggaa acgatgaggc
    30061 tcatcactcc gcagccgcgt tcgggttatt ttgtcgcaca acgtaaagcc cagccgcctg
    30121 taccgccgat gacgcgtccg gtgcagcgcc cggtggaaat tacccagtgg gatcaggtgc
    30181 tggatatgct ggaggcgcat agcgacaatt ccattgttcc gttaagcaaa agcacgccgg
    30241 atgtcgaaac gcccagcctg aaaccgctgt ggcgtgagct aagccgggtg gtgcagcata
    30301 atctgcaaac cgttctcggt tatgacttgt tagccggtca gcgagtgttg cgggagcaga
    30361 ttgcccgcct gatgctcgac agcggctcgg tggtcaccgc cgatgacatc atcatcacca
    30421 gcggctgcca taactcgatg tcgctggcgt taatggcggt gtgtaaaccg ggcgatattg
    30481 tcgcggtcga atctccctgt tattacggtt cgatgcagat gctgcgaggc atgggcgtga
    30541 aagtgattga aatcccaacc gatccagaaa ctggcatcag cgttgaagca ctggaactgg
    30601 cgctggaaca gtggccgatt aaaggcatca ttctggtgcc aaactgtaat aatccgctgg
    30661 gatttattat gccggacgca cgcaaacggg ccgttctctc tctcgctcag cgtcatgata
    30721 ttgtgatttt tgaagatgat gtctacggcg aactggcaac ggagtatccg cgcccgcgga
    30781 ccatccattc atgggatatc gacgggcgag tgttgttgtg cagctcgttc agtaaaagta
    30841 ttgctccagg cctgcgcgtg ggttgggtcg caccggggcg ttatcacgat aaactgatgc
    30901 atatgaaata cgccatcagc agctttaatg tgccgtccac gcaaatggcg gcggcaacgt
    30961 ttgtgctgga aggtcactat catcgccata tccggcggat gcggcagagc tatcagcgca
    31021 atttggcgct ttatacctgc tggatacggg aatattttcc ctgcgaaatc tgtattacgc
    31081 gcccgaaagg cggattttta ctgtggatag aattgcctga acaggtcgat atggtctgcg
    31141 ttgcgcggca gctgtgccgc atgaatatcc aggtggcagc aggctcgatt ttctcggctt
    31201 ccggcaaata ccgtaattgt ctacgcatca actgcgcttt gccgctcagc gaaacctatc
    31261 gcgaagcact aaagcaaatt ggcgaggccg tgtatcgggc aatggaataa attcgaatta
    31321 gtggcgaagc acctgatttc ctctcacgaa tcgcctgtaa ctcgttgtat ctttgcaggg
    31381 tcatcgccac gttccagcat ggcttaggca aagtgtgctt cgttcatacc ggatgcggcg
    31441 tgaacgcctt atccggccta caaaccgcgc aaattcaata gattgcagag gaaatgtagg
    31501 tctgataagc gtagcgcatc aggcagcttt gcgtttgtca tccgtctctg attagtattc
    31561 gatccatgga tcgcgcttga tttcaggcaa gggctgctct aatgcctgca tttgctctgt
    31621 cgaaagtccg gtaatctttt gtatccattc aggatctgcc ccattctgca acaacaatct
    31681 aaggaggcgt tgttcccctt cttctttccc cttttccatc cccaacagcc atccatcatt
    31741 ataaattcgc tctgcaatcg tcattattcg ctccctgtgt tgtggcattc gacgggtaag
    31801 ttcgctgata aacttcttaa aacgcgcttc atcgccagtc agtaaaatgt aatttaacag
    31861 cgcagttatc tggctgtcat tagcacactc tgtaaccagt aatattacca gttgatcgat
    31921 aagccccatc agatcgcgct ggcgaatatg cttttggatc aactccaaca gggcgactct
    31981 gcggtgctgc acaatctcgt cgtctggcac gacagtgaca tccaccagcg ggaacgctgc
    32041 ggtataaagc ttccgtgcgg tggtcgggtc agcaaattcg tccagccagc acagggacca
    32101 gggataagga ctacggctac cgtgataaaa cagcatcggg atgaccagcg gtagcggccg
    32161 gcgtttatca tgctcgatat ggcgctgcat caccgccatg gaatatcgca tcaggcgaaa
    32221 ggccatatgg atatcctcgc ggctctgatg ttcaatcact acgtaaatat aaccatcacc
    32281 ttcacgggtc tttaccgacc acagaatatc ggagtgtagc gcccgcaatt tttcatcgac
    32341 gaagctggcg gattccagtt ttaagctgtc gagatcgcac agttcacgta aatctttggg
    32401 taagtggatc tccataaaat cccgcgcggt gtcagggtgc gtgagaaagg atttaaataa
    32461 tgcgtcatgc ggcgtgctgg ttgtgaagtt tgtcatggcg atccgtcacc tgtagataag
    32521 atggcccgac tgtagcgact aacaaaacca ttctcacccg gcaatttctc gttccccgag
    32581 gcgctttcca ggctatttgt gttcctctgc aaacgataca tttttccgga aacaggctcg
    32641 cgttttacat ttcctgtacc cggcagatat tcccctttcc accgcgctct tttgattaac
    32701 cgcaaatttt aattacactt aaggtgtata ttttctatgc aacccatcaa ttaaagaggt
    32761 gtaatgtgct gatgactatt tgtaatcgtt atacattctg acctggagtc agaaggtatt
    32821 tctctgtctg tgtgttcaca ggcagtgtgg ttgattacat gaaattcagt acatttgtcc
    32881 ttctcgcctg cctttcgtca ttaccgacgg tattgaattt cgttttcccc gttggggttc
    32941 tccggacaag gagttgtttg ttatgccacg tttttttgcc cgccatgccg ctacgctgtt
    33001 tttcccgatg gcgttgattt tgtatgactt tgccgcctat ctttcgacgg atctgatcca
    33061 gccggggatc attaatgtgg tccgtgattt taatgccgat gtcagtctgg ctccggctgc
    33121 cgtcagtctc tatctcgcgg ggggtatggc gttacagtgg ctgctggggc cgctttccga
    33181 caggattggc cgcaggccgg tgctgattac cggggcgcta atatttaccc ttgcctgcgc
    33241 tgcgacaatg ttcacaacgt ctatgacaca gtttcttatc gcgcgtgcaa ttcagggcac
    33301 cagtatctgt tttattgcta ccgttggtta tgtcacggtg caggaggcgt tcggacagac
    33361 aaaagggatt aagttgatgg cgattatcac ctccatcgta ctgattgcgc cgattattgg
    33421 cccgctttcc ggcgcagctc tgatgcactt tgtgcactgg aaagtccttt ttgccatcat
    33481 tgcggttatg gggtttatct catttgttgg cttactgctg gcgatgccag agacggtgaa
    33541 gcgcggcgcg gttccattta gcgccaaaag cgtcttgcgc gattttcgta atgtcttttg
    33601 caaccggctg ttcctctttg gcgcagcaac catctcttta agctatatcc cgatgatgag
    33661 ttgggtggct gtctcgccgg tgatcctgat tgacgcaggc ggcttaacaa cttcgcagtt
    33721 cgcctggaca caggttccgg tgttcggcgc ggtgattgtc gcgaatgcca tcgtggcgcg
    33781 tttcgttaaa gatccgaccg aaccgcgctt tatctggcgt gctgtaccca ttcaactggt
    33841 cggcctcgcg ctgttgatta tcggcaatct gctgtcaccg cacgtctggc tgtggtcggt
    33901 gctgggcacc agtttgtatg ctttcgggat tggtttgatc ttcccgacct tattccgctt
    33961 tacgcttttt tccaataact taccgaaagg aaccgtctcc gcatcgctga atatggtgat
    34021 cctgatggtg atgtctgtct cggtcgaaat cggccgctgg ctgtggttta acggcggtcg
    34081 cttgccgttt catctgttag ccgtcgtggc gggcgttatc gtcgttttca ccctggcagg
    34141 attgctcaat cgcgtgcgcc aacatcaggc tgccgagctg gcggaggagc ggtgattttt
    34201 gcgcgatcct gccgtcaggc tctattctta acgttatgaa taaactcatt gaactcagac
    34261 gcgccaaaat gttggcgctc tctttactgc ttatcgccgc tgctaccttt gtcgttacgc
    34321 tgtttttgcc gcccaatttt tgggtgagcg gcgtgaaggc gattgctgaa gcggcgatgg
    34381 tcggcgcgct ggcggactgg tttgcggtgg tggcgctgtt tcgccgcgtg ccgattccga
    34441 tcatttctcg tcatacggcg attatcccgc gtaataaaga ccggattggc gaaaatctcg
    34501 gccagttcgt gcaggaaaaa tttctcgata cccagtcgct ggtggcattg attcgacgtc
    34561 acgaaccggc gttgttgatt ggcaactggt ttagtcagcc agaaaacgcc cgccgcgttg
    34621 gtcagcatct gttgcagatc atgagcggtt ttcttgaact gaccgatgat gcgcgtattc
    34681 agcgcctgct taagcgcgcg gtccatcggg cgattgataa ggtagatctt tccggcacca
    34741 gtgcgttgat gctggagagt atgaccaaaa acgatcgtca tcaggtgctg ctggatacgc
    34801 tgatcgcaca gttgatcgcc cttctccagc gcgataaatc gcgcaagtta atcgcccagc
    34861 aaattgttcg ctggctggag agcgagcatc cactaaaagc caaaattctc cccaccgaat
    34921 ggctgggcga acatagcgcg gagttggttt ctgacgcggt gaattctttg cttgatgata
    34981 tcagccgcga tcgtgcgcat cagatccgcc atgcgtttga tcgcgccact tttgccctga
    35041 tcgacaagtt gaaaaacgat cctgaaatgg cagcgcgagc tgatgccgta aaaagctatc
    35101 tgaaagaaga tgaagctttt aaccgctatc tcagtgaatt gtggggggat ttacgggaat
    35161 ggctgaaagc ggatatcaac agtgaagatt ctcgtgtgaa agaacgtatc gcgcgggcgg
    35221 gtcaatggtt tggcgaaacg ttaattgccg atgatgcctt gcgggcgtcg ttaaatggtc
    35281 atctggaaca agccgcgcac cgcgtcgcgc ctgagttttc cgcattcctg acgcgccata
    35341 tcagcgatac ggtaaaaagc tgggatgcgc gggatatgtc gcggcaaata gagttaaata
    35401 tcggcaaaga tctgcagttt atccgtgtca acggtacgct ggttggcggt tgtattgggc
    35461 taattttata tttgttgtcg cagctcccgg ccttgttccc cctcggcaat ttttagaaat
    35521 ccatgaaaaa ctaagtagct aaccgcgaga gagatcaaat aaaccacact aaaagaatgg
    35581 taataatgtg gcctcttttc attatctccc gtggtacggg gaaggaaaat catgtcactt
    35641 gtcaccgatc tacccgccat tttcgatcag ttctctgaag ctcgccagaa aggctttctc
    35701 accgtcatgg atctcaagga gcgcggcatt ccgctggttg gcacttactg cacctttatg
    35761 ccgcaagaga tcccgatggc agccggtgcg gttgtggttt cgctctgttc cacctctgat
    35821 gaaaccattg aagaagcgga gaaagatctg ccgcgcaacc tctgcccgct gattaaaagc
    35881 agctacggct tcggcaaaac cgataaatgc ccctacttct acttttctga tctggtggtc
    35941 ggtgaaacca cctgcgacgg caaaaagaaa atgtatgaat acatggcgga gtttaagccc
    36001 gttcatgtga tgcagctgcc gaacagcgtt aaggacgata cctcgcgtgc gttatggaaa
    36061 gccgagatgc tgcgcttgca aaaaacggta gaagaacgtt ttgggcacga gattagcgaa
    36121 gatgctctgc gcgatgccat tgctctgaaa aaccgcgaac gtcgcgcact ggctaatttt
    36181 tatcatcttg ggcagttaaa tcctccggcg cttagcggca gcgacattct gaaagtggtt
    36241 tacggcgcaa cattccggtt cgataaagag gcgttgatca atgaactgga cgcgatgaca
    36301 gcccgcgttc gtcagcagtg ggaagaaggc cagcgactgg acccgcgtcc acgcatttta
    36361 atcaccggct gcccgattgg cggcgcagca gaaaaagtgg tgcgcgcgat tgaagagaat
    36421 ggcggctggg ttgtcggtta tgaaaactgc accggggcga aagcgaccga gcaatgcgtg
    36481 gcagaaacgg gcgatgtcta cgacgcactg gcggataaat atctggcgat tggctgctcc
    36541 tgtgtttcgc cgaacgatca gcgcctgcaa atgctcagcc agatggtgga agaatatcag
    36601 gtcgatggcg tagttgatgt gattttgcag gcgtgccata cctacgcggt ggaatcgctg
    36661 gcgattaaac gtcatgtgcg tcagcagcac aacattcctt atatcgctat tgaaacagac
    36721 tactccacct cagatgtcgg gcagctcagt acccgtgtcg cggcctttat tgagatgctg
    36781 taaggagtgg cagtgacata ttcgattggc attgattctg gttctaccgc caccaaaggg
    36841 atcttactgg cagacggcgt gattacgcgc cgtttcctcg ttccaacacc ctttcgtccg
    36901 gcaacagcaa ttactgaagc ctgggaaact ctgcgcgaag ggttagagac aacgccgttt
    36961 ctgacgctca ccggctatgg acggcaactg gtggattttg ccgataaaca agtgacggaa
    37021 atctcctgtc atgggctggg cgcacggttt cttgcgccag caacgcgcgc ggtaatcgac
    37081 atcggtggtc aggacagcaa agtgattcag cttgatgatg acggtaacct gtgcgatttc
    37141 ctgatgaatg acaaatgcgc ggcgggcacc gggcgtttcc tggaggtgat ctcgcgcacg
    37201 cttggcacca gcgtcgagca actcgacagc attaccgaaa atgtcacgcc gcacgccatc
    37261 acgagtatgt gcacagtgtt tgccgaatca gaagtgatca gcctgcgctc agcgggcgtc
    37321 gcgccagaag cgattctcgc cggagtgatt aacgcgatgg cgcggaggag tgccaatttc
    37381 attgctcgtc tctcctgtga agcgccgatt ctgtttactg gtggcgttag tcattgccag
    37441 acgtttgccc ggatgctgga atctcatctg ggaatgccgg taaataccca tcctgatgcg
    37501 caatttgctg gcgcaattgg cgcggcggta attggtcaac gagtgaggac acgccgatga
    37561 aagagttttt atttttattt cactccacag tcggcgtcat acaaacccgc aaagcgttgc
    37621 agtcagcggg catgactttt cgcgtcagcg atattccccg tgatttacgc ggcggctgcg
    37681 ggttatgcat ttggctgacc tgtccccccg gcgaggaaat acaatgggtg atccctgggc
    37741 tgactgagtc aatttattgt cagcaggatg gtgtctggcg ctgcattgca cattaccggg
    37801 tttctccgcg ttaaggattc attaaggtcg ctacgtatac ttctcgatat cttattgatt
    37861 tgccttaaag ggattaccgt gacaaaaagt atttcgttaa gtaaacgcat ctccgttatt
    37921 gtgattcttt ttgccattgt ggcagtgtgt actttcttcg tgcaatcttg cgcccgtaaa
    37981 agcaatcatg cagcatcatt tcagaattac cacgccacca ttgacggcaa agaaattgca
    38041 ggcataacga ataatatctc ttcgctgacc tggtcggcac aaagcaatac tctttttagc
    38101 actatcaaca aacctgccgc cattgttgaa atgaccacca atggtgattt tatccgcaca
    38161 attcctctgg attttgttaa ggatctcgag acgatcgaat acattggcga taatcagttt
    38221 gtcattagcg atgagcgcga ttacgccatt tatgtgattt cactgacccc aaactcggaa
    38281 gtaaaaatcc tcaaaaaaat aaaaatcccg ctgcaagact ccccaaccaa ttgcggtttt
    38341 gaagggttgg cttattctcg ccaggatcac actttctggt tttttaaaga gaaaaatccg
    38401 attgaggtct acaaagttaa cgggctgtta agtagcaatg aattacacat cagcaaagac
    38461 gaggcactac aacggcaatt tactttagat gatgtgtccg gcgcagaatt taaccagcaa
    38521 aaaaatacgc ttctggtgct gtctcatgaa tctcgcgcac tccaggaagt aaccctggtt
    38581 ggcgaggtta tcggtgaaat atcactaacc aaaggtagtc gaggtttgtc gcataacatc
    38641 aaacaggccg aagggattgc gatggatgcc tctggtaata tttatatcgt tagcgaacca
    38701 aatcgttttt atcgctttac ccctaaatca tcgcattaac aggctggcat atttcacctt
    38761 tttgttatta acttgttaac aacaaaaatg acatacaagg ttaaacatgc cttcgtcctc
    38821 gcatcccgta gaacgctttt ctttcagcac cgcgcttttc gggatgctgg ttctgaccct
    38881 aggtatggga ttaggtcgct tcctttatac gcctatgttg cccgtcatga tggcggaagg
    38941 ggcgttttca tttagccagc tctcgtggat tgccagcggc aactatgcgg ggtatctggc
    39001 tggcagtctg ctattttcgt ttggcgcatt tcaccagccg tcgcgcctac gcccatttct
    39061 gttggtttct gccctggcga gcgggttgtt gatcctcgca atggcatggt tgccgccatt
    39121 tattctggtg ttattgattc gcttcctggc gggtgtcgcc agcgccggta tgctgatttt
    39181 tggttcgacg ctgattatgc agcacacgcg ccatcctttt gtgctggcag ctttgttttc
    39241 tggcgttggc attggcattg cactgggcaa cgaatatgtt ctggcaggcc tgcattttgc
    39301 cttctcctcg caaacgttat ggcaaggcgc cggggcgctt tctggcatga tgctgattgc
    39361 acttacgctt ttaatgccct cgaaaaaaca cgccatcgca ccaatgccat tggcaaaaac
    39421 ggagcaacag ataatgaact ggtggttact ggctattctg tacggcctgg cgggatttgg
    39481 ttatatcatc gtcgcgactt tcctgccgct catggcgaaa gatgcaggct caccattgtt
    39541 aaccgcccat ctctggacgt tagtcggctt atcaattgtg ccaggctgct ttggctggct
    39601 atgggcagca aaacgttggg gggctttgcc ttgcctgacg gcgaatttgc tggtgcaggc
    39661 tatcagtgtg ctgcttactc tcgccagcga ctctcctctc ttgcttatca ttagtagtat
    39721 cggttttggc ggtaccttta tgggcacgac atctctggtg atgactatcg cccgccaact
    39781 gagcgttccg ggaaatctca atcttttagg ctttgtgacg ctcatttacg gcattggaca
    39841 aatcctcggc ccggcgctga ccagtatgct cggcaatgga acatcggcgc ttgccggcgc
    39901 cactctctgc ggcgcggcgg cgttgtttat tgctgcgctt atcagcacag tacaattatt
    39961 taagctgcaa gtggtcactt catgaggagt gtttccctta ccactcaaga aactcaacag
    40021 caaccgttga tgtcagccag accccgtttt ccgcttgcca gaaagggatg cctcgtttcg
    40081 ccatttcctg tgctttgacg gttaagataa ccggcgatcc gtgtcttgct cccactttgc
    40141 gtgccgttgc ttcatcagca gagagatgta cgtaatgacg ctcccctgca atcagcccct
    40201 gtttttttat ctcgtcaagg aaacggcttg ccgtaccgtg gtagagaaac tgtggcggtg
    40261 ttttttcggc aaaggaaatg gcgacctgcg aagtcgaatg cccctgaacc gcgcggatgc
    40321 acctaccatc actggaataa ctaaaacgtt ttttatcact ggtagcgacc acggtatcca
    40381 gcaatgcacg ggttaaccgc ttgcccgctt tttgggcaca aagtatgagc ttatcgatat
    40441 ccgcccatcc ttcacggtcc agtacgatac ctatcgcctc cggtttgtgt cggaggacaa
    40501 aacttaaaaa cttgctcgta tccgccagct ctttttcgtt gtactttgcc atcgcgtttt
    40561 cgtccctgga ttttctgttt ttatttactg atgtcattgt cactgggttt atgaattttc
    40621 tgcaactcaa tcctgcgcag agccttttcc acacagcata atttttccgc tatgcaaaaa
    40681 acgcatagcc agaaacatac ttgcattttc cccataccca catggctgat aaggtttttc
    40741 cgctccagga attttcctgg ctcaagcaac acacaaacac aaaaaaacaa tggggattgt
    40801 tatgacacaa caaggggatg ctgtagccgg ggagctggcg acagaaaaag tgggcattaa
    40861 agggtatctg gctttttttc tcaccataat ctttttttcc ggtgtttttt ccggaacaga
    40921 cagctggtgg cgcgtttttg atttttccgt tctgaacgga tcgtttgggc aactgcctgg
    40981 tgcgaacgga gccaccacct catttcgcgg ggcgggtggc gcgggggcta aagatggctt
    41041 tctctttgcg ctggagctgg caccgtcagt cattctctct ctggggatta tttcaattac
    41101 cgatggtctt ggtggattac gcgccgcgca gcagttaatg acgcctgtgc taaaaccgtt
    41161 gttgggcatt ccagggatct gctcgctggc gctgatcgct aacctgcaaa acactgatgc
    41221 cgccgccggt atgaccaaag agctggcgca ggaaggggaa attaccgaac gcgataaagt
    41281 gattttcgcc gcatatcaaa ccagcggtag cgccatcatc accaactact tctcttccgg
    41341 cgtcgcggtg tttgcctttc tgggcacgtc cgtaatcgta cctttagccg tcatcctggt
    41401 gtttaaattt gtcggagcca acattttgcg cgtctggctt aacttcgaag aacgtcgcaa
    41461 cccaacgcaa ggagcgcaag catgacaact caggtccgca aaaacgtcat ggatatgttt
    41521 atcgatggcg cgcgccgtgg ctttactatc gccacgacca acctactgcc aaacgtggtg
    41581 atggcgtttg tcatcattca ggcgctgaaa attaccggcc tgctcgactg ggtggggcat
    41641 atctgtgaac ccgtaatggc gctgtgggga ttacccggtg aagcggcaac cgtcctttta
    41701 gccgcattaa tgagtatggg cggcgcagtc ggtgttgccg ccagtctggc gaccgccggc
    41761 gcattaaccg gtcacgatgt caccgtttta ctgcccgcga tgtatttaat gggcaacccg
    41821 gtgcaaaatg tcggtcgctg cctggggaca gccgaagtga atgccaaata ttacccgcat
    41881 atcattacgg tctgcgtgat taacgcattg ctgtcgatct gggttatgca gcttattgtt
    41941 taacaaggag ttaccatgat tgattatacc gcagccggtt ttaccctgct gcagggagcg
    42001 catttgtatg cgccggaaga tcggggaatt tgcgatgtcc tcgtcgctaa cggcaaaatt
    42061 atcgccgttg ccagcaatat cccttctgac attgtaccgg actgcacggt tgtcgatctc
    42121 agcgggcaga tcctctgtcc tggttttatt gatcaacacg tccatttgat tggtggtggc
    42181 ggcgaagcag gtcccacgac gcgcacgcca gaagtggcgc taagtcgcct gacggaagcg
    42241 ggcgtcacgt cagtggttgg tctgctgggc accgactcta tctctcgcca cccggaatcc
    42301 ctgctcgcca agacccgtgc gctcaatgaa gaaggcatca gcgcctggat gctgaccggc
    42361 gcttatcatg tcccttcccg caccattaca ggttccgtgg aaaaagacgt ggcgattatc
    42421 gatcgtgtga ttggcgtgaa atgcgccatc tctgatcacc gttctgccgc accggacgtt
    42481 tatcacctgg ctaatatggc ggcggaatcc cgcgttggcg gtttgctcgg cggtaaacct
    42541 ggcgtcaccg tgttccacat gggcgacagc aaaaaggcgt tacagcctgt ctatgacctg
    42601 ctggaaaact gcgatgtgcc gatcagcaag ttgctgccga cccacgttaa ccgcaacgtt
    42661 ccgttgtttg agcaggcgct ggagttcgcg cgcaaaggcg gcactatcga tatcaccagc
    42721 agcattgacg aaccggtcgc ccctgccgaa ggtattgccc gcgccgttca ggcgggtatt
    42781 ccgctggcac gcgtcaccct cagctccgac ggcaacggta gccagccgtt cttcgatgac
    42841 gaagggaatt taacccatat cggtgttgcc ggttttgaaa cgttgctgga aaccgtgcag
    42901 gtgctggtca aagactatga tttcagtatc agcgatgccc tgcgcccgct caccagtagc
    42961 gtagccggtt tccttaacct gaccgggaaa ggcgaaattc tgccaggcaa tgatgctgac
    43021 ttgctggtca tgacgccaga actgcgcatt gagcaggtat acgctcgcgg caaactgatg
    43081 gtcaaagacg gcaaagcctg cgtgaaagga acgtttgaaa cggcttaaac ggttgtcagc
    43141 cgcccgaatg gacgtagaat gcccgcaagg gcggctgaca gagtaaaacg taatggatga
    43201 ctgtggtgcg attttgcata atattgaaac caaatggctt tatgattttc tgaccctgga
    43261 aaaatgccgc aatttttccc aggcggcagt cagtcgcaac gtctcgcaac cggcattcag
    43321 ccgccgcatc cgtgcgctgg aacaggcgat tggtgttgaa ttgtttaacc gccaggtaac
    43381 gccgctgcaa ctctcagaac aagggaaaat cttccattcg cagatccgcc atctgttgca
    43441 acagttagag agcaatctgg cagagctgcg tggcggcagc gattacgcgc aacgaaaaat
    43501 caagatagcc gctgcacact ctctttccct cgggctgtta ccgtccatta tcagccagat
    43561 gccgccgctc tttacctggg caattgaagc tattgatgtc gatgaagcgg tcgataaact
    43621 gcgtgaaggg caaagtgact gtattttttc ctttcacgac gaagatttgc tggaagcgcc
    43681 gtttgaccac attcgcttat ttgaatctca attgttccct gtctgcgcca gtgacgaaca
    43741 cggagaagca ctttttaacc tcgcgcagcc acactttccg ttactgaatt acagccgcaa
    43801 ctcctacatg gggcgattga ttaatcgcac cctgacgcgc cacagtgagt taagtttcag
    43861 cacctttttt gtctcttcga tgagcgagct tttaaagcag gttgccctcg acggctgtgg
    43921 gattgcctgg ctgccggagt acgccatcca acaagaaatt cgcagcggga agctcgttgt
    43981 actgaatcgg gacgaactgg tgatcccgat tcaggcctat gcataccgaa tgaatacccg
    44041 catgaatcca gttgccgaac gcttctggcg tgaactgcgc gagctggaga ttgtgcttag
    44101 ctgacattct ccagcatcgc actacaccaa ccgccattat cccagaccag atcaaacgtt
    44161 agcggttcac cgtcgtggta cagcattatc tcaaggctga tcgccagagt gtggcagtac
    44221 gcttctttaa aattaacctg caacgccgca atccgtggct catgaaataa cagtgcagaa
    44281 cgcagctgac ggcaatacca gctctggaga tgtttgtcgc tctggttatg cacttgccac
    44341 tctggcatgc ctgaaacaag taatgacggt aatgccgaat gaggagaatt aaaaagagtg
    44401 aggagatcgc tgcataccga tttacgcagc aatgacgttt tattgcctga aatttcaggt
    44461 aaaacagcct gaagtgattg gcgcatcatt ttgcgcactc ctgacgttta gcaacatatg
    44521 cggcttgccc tgaacaccgg gcaccactgt attgatgctc attttccata gccattccct
    44581 tttgttcatt acatccatgt cagtctgaga caggcctgaa tcctgtcagc aaagtataaa
    44641 accttacttg cctatgaata tctactgact tttcaggaaa aaagtcatga tttgataaaa
    44701 atcatcaaac tcaaaaaaaa accgctagta tattctgaat attccgaata gttacgtaga
    44761 tgaaaatact tgtcaccgct gctacttaat tccggtggga aattatttat ttccgcataa
    44821 tcagccatgt attatttgaa acaattagtt tgtaaataat ataaattgat aacatatcaa
    44881 atacatcaaa aataccattc acaggcatta ttaatataga catcaatata gcaacatcaa
    44941 attccaatta gtaataagat atgcttttta attgcatgaa aataatttca aatgagcgtt
    45001 ttttgatact ttagagtgtt tcctaccctt taatatcacc gtgtagagtt gatatcacta
    45061 caacagaaac atgcatgtca tttgtttact tctcttttta ctacagaaaa atttatctat
    45121 ttttctaaac atcagtgaaa tactcctaaa aggtaactat tatgtctatg ccattaagca
    45181 atgcccttca gagtcaaatt attacagata atcacttcct tcatcaccca aaagtcgaca
    45241 atgagttaac acgtaaatat gaacgagctc gcctggatac ggaaaatatc tatcttctgc
    45301 ctcttgctcg tggcaataac cataattacg atggaaaaag cgttgtcgaa attagaaaac
    45361 ttgatattag taaagagtcc tggccattta attatgtgac agaagcatgc agagaatccg
    45421 atggtattac aacaacagga cgtatgctct atcgaaactt gaaaatcacc tcagctttag
    45481 acgaaatata tggtggtata tgtaaaaagg cgcatgctgc tacagaatta gcagaaggtt
    45541 tacggctaaa tttatttatg aagagccctt tcgatcctgt tgaggactat acagttcatg
    45601 aaataacatt aggccctggt tgcaatgttc caggttacgc cggaaccacg ataggatata
    45661 tttcaaccct tccagtttct caggcaaaaa gatggaccaa tgagcagcca agaattgata
    45721 tctatattga tcagataata actgtctcag gggtagcaaa ttcttccgga ttcgcccttg
    45781 ccgcattact taatgctaat attggaatgg gaaatgaccc aataattggt atagaagcct
    45841 atcccggtac cgcggagatc catgcaaaaa tgggatataa cgttatcccc ggtgatgaag
    45901 atgcgccact caaacgcatg actctgcaac cgtcatctct tccagaactg tttgaattaa
    45961 aaaatggtga atggaattat attggtaaat agccactgag aagaatgagt actaatattc
    46021 aggttataca gatatttaaa caataccctg caatttacaa agtaatactc attgcgactt
    46081 cttaatttaa gaaagcgcaa ggaacgttta cccttgcgct tattataata agtcaggcgt
    46141 cgactttatc cagcggccat gaatcaaaca gatagccgtc aaaataaata tcgtcaacgt
    46201 tcgagaattc cagcagacgt tgcttaacgt tttccagatg ctgccacatt gccagcttcg
    46261 ccgctcgggc atcttttttg attaacgcgg cgaggatctg tttgtgatcg cccaaccact
    46321 ctttgcgata caggctgtca tccagatggc tgtgcaactg gatccacatt gggttgtttt
    46381 cccgccactg ccaggactga cggaacagct ccaccagcat gctgttatgc gttgcttcgg
    46441 caatagcgag atggaactgc atgtcaccgc tttcgctgct gcccggcgca ctggaagcca
    46501 gttcacgctc ttccagttgc aatgcctgac gcattttgac gatatcttcg cgggtagcct
    46561 gcagggcggc aaactcggcg atgttacttt ccagtaactg acgcgcctgt aacagctcaa
    46621 aaggacccgc atcattgcag acgttggcat ccggactgtc tgtgttgtgg ctgcctgagc
    46681 tatcaagaac atagataccg gcaccccggc gcacttccac cagccctttg atctccagca
    46741 tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag catttctgca atttcacgct
    46801 ccggcggcag ccgttcgcca gggttgtacg gcgtctttat gatcagatcg cggatcatcg
    46861 ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga tttcatggtg ttgtgtccgt
    46921 taggtgcggt taatcaaatt tttgccggat gcggcgtgaa cgccttatcc ggcctacaac
    46981 gggcaatttt acggcccccg ttaatctcaa actataccat tagcactgga agttggcacg
    47041 tacaggtggg caaaaaaacg aaaactggtc aaccaaatat cagtttgatt gaccagtaag
    47101 tctgtagggt aatcagttag ttacagcgca gccacacact cgcgcgcacc gcgttcgcac
    47161 agctgctgat atgccgccgt cactgcgcca acaaagtcgg cattctgcgg cagatcatcg
    47221 gcaaaaatac cgctcaggcc cagcagcgct ttcgcgcggt ctgcgccctg atactgcgcg
    47281 ttgatcttct ggaactccgc cagcatcggg tcgaccacgt caatggcatt accctgctca
    47341 tccacgccct gggtgtaacg catccagcca gccacgccca gcgccaggtg acgccagctg
    47401 ccgccgtttt gcaggtgcag acgcaccggg tccagcagac gctgcggtaa cttctggctg
    47461 ccgtccatcg caatctgcca ggtacggtga cgcagagacg ggttgctgaa acgctcgatc
    47521 agcaacgtcg cataggcgtt caggtccgta ccttccggca tcgacagcgt tggcgcttgt
    47581 tcctgcatca tcagggagaa ggccgctttg cgataagccg ggttagtcat ggtgtcggca
    47641 atggtttcat agccgccgag gtaaccgagg tacgccagaa aagagtggct gccgttcagc
    47701 atacgcagct tcatcatttc gaacggcaca acgtctgcaa cgaactgtgc gcccacttta
    47761 tcccagtccg ggcgaccatt aacgaagtta tcttcaatca cccactgacg gaacggttcg
    47821 caggcaatgg cgcacgggtc gtaaacaccc agctggtcag caatttcctg taaggtttct
    47881 ggcgtcgccg ctggaacgat gcggtcaacc atggtgcatg ggaaggtgac gttctcttca
    47941 atccatgccg ccagctgcgg atcgcgtgcc tgagccagtc ccagtaccgc gaccttcgcc
    48001 acatgaccgt tttcacgcac gttatcgcag gacatcaccg taaacgcttt caaccctttt
    48061 tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg cggacttcgg cgcagtcggg
    48121 ttttccagat cgtgcttgat cagtgggtta ttgagatcca gctgaccgct tgccgcatca
    48181 gcgcagtagc ctttttccgt gaccgtcagg gagacaatcg ccgtctgcgg acgcgccatc
    48241 gcgttgagaa taccttcgca accgtcgatt tccggatgca gcgcttcttt catcgaaccg
    48301 ataattttca gctcggtgct ctctgcgcct ttttccgcta cggtgtacag cagttgctgt
    48361 tttttcaggt tttcgatcag cacgcggtcg ttgcctggca tcaggttaac ttcgcagatg
    48421 ccccagtcgc tgtcggtgct ttccagcaga tgatgggtat acagcgcctg gtgcgcgcgg
    48481 tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca gacgagaatg atcccatgac
    48541 gggcgggcaa ccggcagatt gctgtcaaca atagtagtca tcgtaaactc cagaatccgt
    48601 cttcaaagag tgacggcaag gaagaaggaa ccggcacggc gcgcagccat gccggtggat
    48661 attaacggct aaagaaagcg cgctggatcg ccagttcgac accgcgaact tcggccaggc
    48721 ctttcagacg accaattgcg gagtaacctg ggttggtttt cttcttcagg tcgtccagca
    48781 tctgatgacc gtggtccgga cgcatcggga tcaggtcttc tttgccttcc gctttacgac
    48841 ggtgttcttc ttcaacaatc gctttcacca cttcgtacat atcaacgtca ccgttcaggt
    48901 gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat ggtggagcgc agatgggtga
    48961 agtaaatacg cggaccgaac tgcttgatca tatcaaccag atcgttgtca gcacgcacgc
    49021 cgtaggaacc ggtgcacatg gtaaaaccgt ttgccatgct gtttacggta tcaaccatcc
    49081 actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc gaggatcggg cgcggcggat
    49141 cgtccgggtg aacagccata cgcacgccaa cttcttcggc aaccggaata atcgctttca
    49201 ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat atctttgtac agctccaggt
    49261 gcttacggaa ctggtcgagg gtgtaccctt cttccgcgcc cggcagacca gcgatgatgt
    49321 tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc gaagcgttca gctgcctgag
    49381 caatttcttc ttcggtgtaa tccgcttccg cgcctggacg tttcaggata tgcatttcga
    49441 atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga gccgtctggc agcacgtatt
    49501 cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt gtagcacacg gtgcgaatgc
    49561 cacactgcgc caggttgcgc agagtctgct gatagttagc gatccactgc tcatagttgc
    49621 cagtgtgggt tttgatatct tcgtgaattg gtacgctttc tacgacagac cacaccaggc
    49681 ctgcgtcttc aacgattgcc ttgcgtttga ggatctcttc tacggaccat acttcgccgt
    49741 tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc cgcctgacgg acatcagcta
    49801 aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg ttccatgatt tttcctcttt
    49861 cacatagtgg gttccacagc aaagagacgc ggaaccctgg agttgtttag gtatccggat
    49921 gaacaaccag accgcagccg tggcgttgca tcggatgaga tggcgtataa gttctacctt
    49981 ttggtaagac atgtcaaccc gaatcctgaa attggttaac cacatcacaa gaattttact
    50041 ctcctggttg ttgctgcaaa ttgcagcaaa aggttatcga tggttattga tcagaggatg
    50101 tgacattcat cgcaacaatg gttgaccaat ttacataaca tgtcgcccaa aataacaacg
    50161 gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac attctctggg tagtattcgg
    50221 cattggcctg atgctggtac tgaatttgaa gttcaaaatc aattcaatgg tggctttgtt
    50281 ggtggcggcg ctgtccgtcg ggatgctggc gggcatggat ttgatgtcgc tgctgcacac
    50341 tatgaaagcg ggcttcggca acacgctggg ggaactggct atcatcgtgg tgttcggtgc
    50401 ggtcatcggt aaattgatgg tcgactccgg cgcggctcac cagatagcgc atacgctgct
    50461 ggcgcgtctc ggtctgcgct atgtacagct gtcggtgatt atcatcggct tgatttttgg
    50521 tctggcgatg ttctatgaag tggcctttat catgttagcg ccgctggtta ttgttattgc
    50581 cgccgaagct aaaattccgt tcctgaaact ggcgatcccg gcagtagcag ctgccactac
    50641 cgcacattca ctgttcccac cgcagccggg tccggtggcg ctggtgaatg cttatggcgc
    50701 ggatatgggg atggtttata tctatggcgt actggtgacg atcccaagtg taatctgcgc
    50761 aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc ccaacgccat cattcctgaa
    50821 agcagatcaa ccggtagata tgaataatct gccctctttc ggcgtttcga ttctggtgcc
    50881 gctgatcccg gcgatcatta tgatctctac caccatcgcc aatatctggc tggtaaaaga
    50941 tacccctgcc tgggaagtgg ttaactttat cggttcctcg ccgattgcaa tgtttattgc
    51001 gatggtggtt gcattcgtac tctttggcac cgcacgtggt catgacatgc agtgggtgat
    51061 gaacgctttt gaaagcgcgg tgaagagtat tgcaatggtg attctgatca tcggtgcggg
    51121 tggcgtgctg aagcagacca tcatcgacac cggcattggc gacaccatcg gcatgttgat
    51181 gtcccacggc aatatctcgc cctacatcat ggcatggctg atcactgtgc taattcgtct
    51241 ggcgacgggt cagggtgtcg tttcggcgat gaccgccgcc gggattataa gtgctgcaat
    51301 cctagatcca gcaactggtc agctggttgg cgtgaatccg gcgctgctgg tactggcgac
    51361 ggctgcgggt tccaacaccc tcacccacat taacgatgca tctttctggt tgttcaaagg
    51421 ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg ggtctgctgg agctggtcaa
    51481 ctccgtggtt gggctgatta ttgtgctgat tattagcatg gtagcgtaaa aggacaccag
    51541 agcctgccaa tggcaggctc tgactgatga caaagtctaa aaatgtgccc agacagtcct
    51601 ctcgcccctc tggggagagg gttagggtga ggggaaaaga ccagcaaagt gcgctttgtt
    51661 tatgccagat gcgacgctaa cgcgtcttat ctggcctaca aagggctaac gtgcaggttt
    51721 ttagcttcag gtaatattgc gtacctgcat tagcaatgcc ctgtgatttc tttattgata
    51781 aacaaaagtc acgccaataa tcgattgcac attccctgca gtcacctgcc ctccggtacg
    51841 tgcgtaattt gccgttaatc ccagacttac cgccgaagtc cctactgctc ctaacgatac
    51901 cgtgttattc gctggaataa tcgtaccgtt gcgcgtcaac tgtacgccga cgccctgcgc
    51961 tggtgaaaac gacgcggtat tggtgaaaat cgagttgccc gcatctgcgg ttgtgccgga
    52021 gaggtaatac cccaggtttt ggcttttcgc acaataaacg gtaagaggaa ttggcactga
    52081 accagggtag tccggcagag taacggtgac atcacgagca gaaacatcgc agccgccagt
    52141 aggcaccacc acatcattat tggcgtaaat attccacaca aactggaaat catcgctgtt
    52201 atagttgttg gtctgtcgca aaataagcac ggcaattaat gagccagctt taatcgccac
    52261 cccgcccgca ctgctcacag gcgtcaaata aagcgccacc ggccacggct tatccgttct
    52321 cgaattataa acaacccgcg gcgtttcgct ggtggtcgga aatggatagc tactgccact
    52381 atattttacg gtcccggaaa aattagataa cacgccgcca taagccgagc ctcgttgcag
    52441 tgtgacatag tctgtaatgg tttccggata atcgttatgg caaaagattt gcgtcgaaag
    52501 atctacgacc aggttttgcc ccacattcac ggcaggcgca aggtttacat aaacattagc
    52561 gctgccaccg ccaataggga tagctgtacc attggcggtt ttacaggcga atgaccaggc
    52621 atttaccgac cagcccatca gcagtacagc aaacagggta ataactcgtt tcattacaat
    52681 catctcttcg ggttcagctg taggtatagg tgatgctaat cactgcctga atggttccct
    52741 gagtggctcc gccatttact gtcaatgctc tgacctgtaa cgggaagtgc gctgattgtg
    52801 aggaatcatc cacctgaact gttttggttg cgccagtatt caatgtgttg ccactgtcat
    52861 cctgtagctc taactggatg ttttgcgcgg tcccctggtt tttataatat ccggtactgt
    52921 cggctgcccc gctgaagctg gcagtgaccc tcgacgttcc caccggacaa ttagtcaact
    52981 caagcgcaac atcatgccag gccgatgccg ccccggcaga catcagactg aaagaataaa
    53041 gatcgccgag atcaaccgtg gcattggtgg tggaaactgt gcacggtttg gcgacgacct
    53101 taccgttcac cgtgatggtg acatcggctg cctgtatcgt cgcacttgcg agcgccaata
    53161 ttgccgccaa tacataccca cgtttgcacc atttcatgag catctccagt tactgatatt
    53221 caagagtgaa ggtagccgtg gcattgatat gccccgcagt gacaggcacc tgtgtcgcca
    53281 ttagccgggc gtaaaaattc aacgtatttg gtttacccgg cgtcagggtc gtccacgaaa
    53341 tcgcggacga tggagcatta aggggtatct gattttgctg ctcattcaga agctgtattc
    53401 ccagtcccga agccgctgat accgtatttt caagtgcaag caggttggca ttgtggctat
    53461 ctgcaacgcc ggtaaaccca acctttacgg cagaaacggc gttaccacag ggtgacagca
    53521 aaatacgaaa tggaacgaca ggagtcgttg cgccaatgtt gttaaattgc ttcgccgcgt
    53581 tttccatcag atcaacagta aaattggttg attcagcggc cacactacag ccgttatctc
    53641 tgacatagcc gcggatagta atcgtgctat ccgcagccaa aacgcgactt accgccagcc
    53701 acaaaaaagc gcacagaaga taaaaaggtt tgtttctcat cacgcccccc ttaacgacat
    53761 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
    53821 atacagtgag cattttcctc ttctcctcat ttcacctgaa cttttcccgc taaaggcatt
    53881 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
    53941 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
    54001 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
    54061 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgccactcta
    54121 ttttcccgat attcagtggc ataaggcagc acggcataac cacgccagtc ggtacgcacc
    54181 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
    54241 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
    54301 ccgtaataga gctgcttaat atcatcacta tggctgtaac cgatattggc attgccgtaa
    54361 ccaccgcgat aattcagcgt ggcgtagcct gtgcttccgc tattaccatc gcctcccccg
    54421 gcatagccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
    54481 ccagccagat tggtcattcg accgttgaga tcgtgtgaca tgctgtagct ggcactggca
    54541 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
    54601 agcgctaaca tctgatcacg tcctttttgc caggcgtttt tcgtcaggct atagctgagc
    54661 gtccagttga tatcttcgaa cgcagtattt aatccagcct ggaattgctc atcgacatta
    54721 ctcgttcccc aataagtttg atggctacca ctcaaataca gtgttgatga gcgcccgagt
    54781 tgctgagtaa cggtgagttg taatttcccg cgtttgttat aagcgaggtt gtaatagtcg
    54841 gtgaatttcg gcttaacctg aataactccg tcctgtgttt cgatgttgta gccattcatt
    54901 cgactgtatg ttgtatcagc gaaattaaaa tatccgctgg tcgaataacg gtaacccact
    54961 aactgaatat tcgtgcctga ctcattgagc gatttgttat agagaaaacg caccgattgt
    55021 ccgtcatgct gactgtcatc gggaagtgtg gaattagcct gcgtcatatc cacagacaga
    55081 gcgcccagtg cccccatgtt tttcccgata ccgaaattaa aagcacgata acgatccgcc
    55141 agttgcgttc caccatatat tgtccagcca gccggaaggc cgtggagtaa tgtactctgg
    55201 aaaaagcggg gtttttcctg ctgcgcattt ccactacggt attctcctgc cgtaatggaa
    55261 taacgagtat gcccttcacg ttgcaaaagc gggactgacg aatagggtac ggtaataatc
    55321 tgcgtgctgc cgtcagcctc tttgatcgtt acctgcaagt caccactatt acctgcggca
    55381 tagatatcgt tgatggtaaa aggccccggt ggcaccgtac tattataaat gtcataccca
    55441 ttttgtttaa tagtgacctg tgcagtacca cgagcaatac cgtggatcac cggggcaaat
    55501 cctctttggc tatcgggtaa catattgtca tctgaggcca attgtgcgcc gcgaaagtta
    55561 ataccatcga aaatatcgcc ctgagtataa ccatcaccca gcgtcagccg ggaacgtaac
    55621 ggtattatgt ctcgctcaag ccaggtattg atatgctgcc atttattttt gctacctgat
    55681 gatctgtcgc tactgttata actccaggtg gtattgtcgc gtaaacgcca cgcaccaata
    55741 tttaacccac tctgtaggtt taaatatgca taatggctgt tacccccaat ccgattctgt
    55801 acactatttc cgctgaaatt ataattgagc aatccggcat taataccggg atcccataac
    55861 tcaggaggaa tataaccacg cgcgcgatta ctcataaatg cctgagggat cgtcaggttg
    55921 agtcgctgct gaccaacatc taaatgcgca gtagcgtcct ggaccattgt ggttaatggc
    55981 acacaggcat catccgcgag gagattcata ccggcgacag aagccatatt cagccccata
    56041 ctggcgagtt gcgcgcgtgt caggcaggga acaatccctt gttcactgtc gcccgtatta
    56101 aatgtgacat cacgcgttgc catataacca ttattcaaat agatatcgac gcgatacgtc
    56161 cctggcggta attcttgccc attttcaaaa cgcgataaat cggccacagc ctggggatca
    56221 tccgctaaaa agcgcgggtt aaaatagagt tcggcagatg acaaaggtgc ctgtgcgaca
    56281 aaagcacagg caacaaagag ccggacaaaa aaaccagcca aatgatgctt acgaatatgc
    56341 aagcattgtg tgtttcgctg gtaaagtctt aaattcagat atgacattat tcctccggtt
    56401 taaaatcacc cggcagtcaa ttctttttat tcaggcgaaa aattccccct gcgttattcc
    56461 attacgcccg tcattttggg ggtaagtgcg ccataatcat ttattgttcg gtaagtaata
    56521 ttgcttcctg catcagaagg caatttaacc gtgctttcgc ccattggagg caccaatgca
    56581 ttttcaagaa cccgggttcc ggcattcaac tctgttaccg tcaggtaata gggtgtcggg
    56641 ttaatcagcg tcagggaatt cgcgctacga cgaaatctta atttttctgc ggcctgatcg
    56701 ggtggcaacg ctaatttagc cggacgatag tacagtttaa tgcggctgat aattgcgagc
    56761 tgtagcgtat tctcagtcaa ttttgattta tccattgacg gaatcgcttt aacattcatc
    56821 cagaataaac tttcccgatc ctgtggcaat tggttattag ttgcatcaag aatacgcaag
    56881 gtattctctc tttttccctt catcgcaaac agaggaggcg tcacgataaa acgaccatcc
    56941 tttacaccat cggcattttc cacccatgat tgaattaaat aggtactatt ttcatcatta
    57001 tttgtcacgg caagttgcac ttgtttttgc cctgccggat aaattacgcg agtcgcacct
    57061 aaggccactc ccgcttcagc gcgtccggca accatcattg ccatgaacat caggatacct
    57121 gccagcaagc agaatgttat ttcctgcgat ttccttacat tgacgttttt attactcact
    57181 gtcctgttcc tgttatcaca ttatctgctg aacaattact gataggttaa agagaaccag
    57241 gcctgggcat tggcgatgcc gccagtaacc cgacgcccgg tagcacgata tttggcgatg
    57301 aaatgtagcg aagtagagcc tgagtaaagc cgtttccagt ttgctggagg acgattaatc
    57361 ggtacgaggt ttccttcatc atcaaacaac gctacgccaa tattggtggc tatccctggc
    57421 ccctctccca cggaaagcac atccggattt ttaccatccg cgacaccgtg aaacgccaca
    57481 cccacacgtt cactcaccac cgtgctacat tcccgtaaat gaataacaaa aggcaccggt
    57541 gcgctatctt cccctaccgc atgaaaccgg ttactgctga tttgccccat attgaccgtc
    57601 atttgtttat caccggcttc aatccggcaa gtttccgcaa taatgacgcc ctgaaattgc
    57661 atatttccgc cgggcagcgt ggtattccat ttatttccgg ccagagcaaa cattggcagc
    57721 aacgacgcta atagaaataa tctcttcctt ttcatcgtta tttttatcgc acaagggtgg
    57781 gcatccctgc ccgtaatgac gtccctgaac ctgggtaggt tattgatact gaaccttgaa
    57841 ggtcgcatcc gcattagcag cacccggggt tgcggcaccg gttgcaaaat aacgcgcctg
    57901 gaacggaatg gtgttggttc cgttattcag ggttgtttct gaactaaatg tcgcaccgtc
    57961 cagcgccagc gcagcacccg ttctgtccag gatctgcaca ccaacgtttg ttgcgctacc
    58021 cgcagctgaa ctctgcagag ccagtacatc agtatgcgta gcatcaattg ccgtacctaa
    58081 gaaggcaaca gcggcttttg tggcaacagt ggtatcgcaa tcattcagct gaatgttaaa
    58141 accaacggca gagctggttg ctccagcctg cttcaggcta gcggtacgaa cctggcccaa
    58201 ctgaacggtt tgatcaacag agcctgcatc aactgcgcaa gcggcgttaa caacttcccc
    58261 tttaaagtga acggtcccac catttaccgt cgtagtatcg gccagagccg ctgcggaact
    58321 gagggacaga gccgacagaa caacgattgc cagagtttta attttcatgc tgctttcctt
    58381 taaaaaacta tttctaaatc gacatgggca gtcgttctgt acactttgtt ttgtcaacga
    58441 gtttgactgc caacactgca cagttttccc ccaaaagatg aaacatttgg ggccattttg
    58501 actcatagag gaaagcatcg cgcacaaact ttttcagttt atttgctggc ttaatattc
//
LOCUS       DAZGXZ010000013        57116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000013 DAZGXZ010000000
VERSION     DAZGXZ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.013"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..955)
                     /gene="yghU"
                     /locus_tag="ACHKJF_000923"
     CDS             complement(89..955)
                     /gene="yghU"
                     /locus_tag="ACHKJF_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP0948330.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            1160..3019
                     /gene="gss"
                     /locus_tag="ACHKJF_000924"
     CDS             1160..3019
                     /gene="gss"
                     /locus_tag="ACHKJF_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP0948331.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            3311..4810
                     /gene="pitB"
                     /locus_tag="ACHKJF_000925"
     CDS             3311..4810
                     /gene="pitB"
                     /locus_tag="ACHKJF_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP0948332.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPHLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(4859..5551)
                     /gene="yghT"
                     /locus_tag="ACHKJF_000926"
     CDS             complement(4859..5551)
                     /gene="yghT"
                     /locus_tag="ACHKJF_000926"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0948333.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLDVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            5725..6501
                     /gene="yghS"
                     /locus_tag="ACHKJF_000927"
     CDS             5725..6501
                     /gene="yghS"
                     /locus_tag="ACHKJF_000927"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0948334.1"
                     /translation="MNQRNMSIINSTPVHVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILEVDGR
                     QPADEILQASLRAIHAALSRSPKWIGDDLTNGCTTNTNCY"
     gene            6470..7228
                     /locus_tag="ACHKJF_000928"
     CDS             6470..7228
                     /locus_tag="ACHKJF_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948335.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            7274..8623
                     /locus_tag="ACHKJF_000929"
     CDS             7274..8623
                     /locus_tag="ACHKJF_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP0948336.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            8623..9447
                     /locus_tag="ACHKJF_000930"
     CDS             8623..9447
                     /locus_tag="ACHKJF_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948337.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            9459..10019
                     /locus_tag="ACHKJF_000931"
     CDS             9459..10019
                     /locus_tag="ACHKJF_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0948338.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            10050..11120
                     /gene="lptF"
                     /locus_tag="ACHKJF_000932"
     CDS             10050..11120
                     /gene="lptF"
                     /locus_tag="ACHKJF_000932"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0948339.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSSANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLKPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRIKPRQGRFSTLLPLTLLFIAIFYGGDVCRSLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            11117..12196
                     /gene="lptG"
                     /locus_tag="ACHKJF_000933"
     CDS             11117..12196
                     /gene="lptG"
                     /locus_tag="ACHKJF_000933"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0948340.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     EILDNLAWQSTFTSMNLEELSMPGNSFSVRQLNHYINYLQETGQPSSEYRLALWEKLG
                     HPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VAALGLPMAFVLAALILVYLFDRQH"
     gene            complement(12231..13403)
                     /locus_tag="ACHKJF_000934"
     CDS             complement(12231..13403)
                     /locus_tag="ACHKJF_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0948341.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(13403..13651)
                     /locus_tag="ACHKJF_000935"
     CDS             complement(13403..13651)
                     /locus_tag="ACHKJF_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0948342.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(13683..14597)
                     /locus_tag="ACHKJF_000936"
     CDS             complement(13683..14597)
                     /locus_tag="ACHKJF_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0948343.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(14594..16315)
                     /locus_tag="ACHKJF_000937"
     CDS             complement(14594..16315)
                     /locus_tag="ACHKJF_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP0948344.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKAL
                     PEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRC
                     VSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPF
                     GYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCY
                     GLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHG
                     IEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGY
                     LYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTVQEKIILQIQCRISD
                     EERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASL
                     HVQESLA"
     gene            16686..17828
                     /gene="yghO"
                     /locus_tag="ACHKJF_000938"
     CDS             16686..17828
                     /gene="yghO"
                     /locus_tag="ACHKJF_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP0948345.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(17835..18599)
                     /gene="glcC"
                     /locus_tag="ACHKJF_000939"
     CDS             complement(17835..18599)
                     /gene="glcC"
                     /locus_tag="ACHKJF_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP0948346.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            18850..20349
                     /gene="glcD"
                     /locus_tag="ACHKJF_000940"
     CDS             18850..20349
                     /gene="glcD"
                     /locus_tag="ACHKJF_000940"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP0948347.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            20349..21401
                     /gene="glcE"
                     /locus_tag="ACHKJF_000941"
     CDS             20349..21401
                     /gene="glcE"
                     /locus_tag="ACHKJF_000941"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP0948348.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            21412..22635
                     /gene="glcF"
                     /locus_tag="ACHKJF_000942"
     CDS             21412..22635
                     /gene="glcF"
                     /locus_tag="ACHKJF_000942"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP0948349.1"
                     /translation="MQTRLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            22640..23044
                     /gene="glcG"
                     /locus_tag="ACHKJF_000943"
     CDS             22640..23044
                     /gene="glcG"
                     /locus_tag="ACHKJF_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP0948350.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            23066..25237
                     /gene="glcB"
                     /locus_tag="ACHKJF_000944"
     CDS             23066..25237
                     /gene="glcB"
                     /locus_tag="ACHKJF_000944"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP0948351.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGAQVIAWVRRFLDESLPLEKGSYQDVVAFKVVDKQLRIQLKNVKETTLRTPAQFVG
                     YRGDTAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRVETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            25593..27275
                     /gene="glcA"
                     /locus_tag="ACHKJF_000945"
     CDS             25593..27275
                     /gene="glcA"
                     /locus_tag="ACHKJF_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP0948352.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGIITLLQAYVFTGMLVS"
     gene            27689..29185
                     /locus_tag="ACHKJF_000946"
                     /pseudo
     CDS             27689..29185
                     /locus_tag="ACHKJF_000946"
                     /inference="COORDINATES: protein motif:HMM:NF023283.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3375 domain-containing protein"
     gene            29191..29940
                     /locus_tag="ACHKJF_000947"
     CDS             29191..29940
                     /locus_tag="ACHKJF_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HFP0948353.1"
                     /translation="MTGFFDRLINRSVTANAGCEPEPSDEEVTDESVEESLASSETRT
                     LKKIREATQELLKYGLLEEASKPNLYRIVLSHPEEVTRILEPLDLDIGIDEIRGLLYV
                     KVRLDETPAQDEWAHLLVRRQRLNLEQSLLVAILRQHFVAWEQESGTGASQAQIAIDD
                     LLPQLQIYLGDPGSESKERTRLLTLLDQLKGHGLVTSPDAHERIVIRPIIAHLADPIN
                     LQALLAWLREQIAQQTSPNDAREKDSSEEDV"
     gene            29937..30140
                     /locus_tag="ACHKJF_000948"
                     /pseudo
     CDS             29937..30140
                     /locus_tag="ACHKJF_000948"
                     /inference="COORDINATES: protein motif:HMM:NF024944.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            31878..33224
                     /locus_tag="ACHKJF_000949"
     CDS             31878..33224
                     /locus_tag="ACHKJF_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HFP0948354.1"
                     /translation="MQNQAASFQKIEQIDFDSIDVPGAKSQLDALRERLENLTRPDSD
                     ASVAKAKLDEAQTVESELDKQLRAANKVTNVLDTELTSARAAERKAQQTAQQGMKEEE
                     RELCASHFPVVTLEQLPDIRDLERQHERGIQHEIERVKAELHRLNIELTKRMSEAKRV
                     DTGALVEAGADLDDITVYLQRLQELTEEALPEKLNRFLDYLNRSSDDGVTQLLSHIEH
                     EVLVIEERLNELNETMFRVDFQPYRYLRLDTKKVVHESLRTLEKAQRQLNAARFVDDN
                     GESHYKALQILVAQLRDACERNRTLGAKALLDPRFRLEFAVSVMDRQSGNVIESRTGS
                     QGGSGGEKEIIASYVLTASLSYALCPAGSRYPLFGTIILDEAFSRSSHAVAGRIIAAL
                     REFGLHAVFITPNKEMRLLRDHTRSAIVVHRRGQNSNMASLSWEELERHYQRRGNA"
     gene            33226..34374
                     /locus_tag="ACHKJF_000950"
     CDS             33226..34374
                     /locus_tag="ACHKJF_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3322 domain-containing protein"
                     /protein_id="HFP0948355.1"
                     /translation="MYSPDELREKLARQWDSAKLRAERLLSPGNWPLCLPIGKPSTKI
                     FAEQTQRVLQHVQRWRQVAVGHVEWEAVSYRASDTPVLMPLRWILNSPSEWINAAADP
                     VVSREFRLLEGIIEQVNPIFHPLLVKHRSLWRHKDPQGVISAATLACRLEPGCAKGLP
                     LRLLSGQGVDTKFIENNISLLTRLLDVRFSGEASEQGLTTFLDAFDESSHWVLVVPLS
                     PGLLPFKKCRVTTAELAETTLPASQVLVVENEQCLHHLPALSDTIAILGCGLDVQWLS
                     SSVLDEKRVAYWGDMDSWGLLMLARARRCRPTLDALLMNRELFEQYASHSAVPEPVIA
                     KEAVPDGLLNEEADFYRYLTRLPCGRLEQEFLPVGIAEEVLLRWGKES"
     gene            complement(34733..34808)
                     /locus_tag="ACHKJF_000951"
     tRNA            complement(34733..34808)
                     /locus_tag="ACHKJF_000951"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34773..34775),aa:Phe,seq:gaa)
     gene            complement(34914..35621)
                     /gene="yqgA"
                     /locus_tag="ACHKJF_000952"
     CDS             complement(34914..35621)
                     /gene="yqgA"
                     /locus_tag="ACHKJF_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP0948356.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            35774..35857
                     /gene="yqgH"
                     /locus_tag="ACHKJF_000953"
     CDS             35774..35857
                     /gene="yqgH"
                     /locus_tag="ACHKJF_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFP0948357.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            36019..38154
                     /gene="speC"
                     /locus_tag="ACHKJF_000954"
     CDS             36019..38154
                     /gene="speC"
                     /locus_tag="ACHKJF_000954"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP0948358.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMEDSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(38204..39460)
                     /gene="nupG"
                     /locus_tag="ACHKJF_000955"
     CDS             complement(38204..39460)
                     /gene="nupG"
                     /locus_tag="ACHKJF_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP0948359.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAVLVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(39662..40741)
                     /gene="mltC"
                     /locus_tag="ACHKJF_000956"
     CDS             complement(39662..40741)
                     /gene="mltC"
                     /locus_tag="ACHKJF_000956"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP0948360.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(40806..41081)
                     /gene="yggX"
                     /locus_tag="ACHKJF_000957"
     CDS             complement(40806..41081)
                     /gene="yggX"
                     /locus_tag="ACHKJF_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP0948361.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(41109..42161)
                     /gene="mutY"
                     /locus_tag="ACHKJF_000958"
     CDS             complement(41109..42161)
                     /gene="mutY"
                     /locus_tag="ACHKJF_000958"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP0948362.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            42322..43041
                     /gene="trmB"
                     /locus_tag="ACHKJF_000959"
     CDS             42322..43041
                     /gene="trmB"
                     /locus_tag="ACHKJF_000959"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP0948363.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            43041..43367
                     /gene="yggL"
                     /locus_tag="ACHKJF_000960"
     CDS             43041..43367
                     /gene="yggL"
                     /locus_tag="ACHKJF_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP0948364.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            43551..44270
                     /gene="yggN"
                     /locus_tag="ACHKJF_000961"
     CDS             43551..44270
                     /gene="yggN"
                     /locus_tag="ACHKJF_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP0948365.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NLAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            44446..45492
                     /gene="ansB"
                     /locus_tag="ACHKJF_000962"
     CDS             44446..45492
                     /gene="ansB"
                     /locus_tag="ACHKJF_000962"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP0948366.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDISKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            45609..46616
                     /gene="yggM"
                     /locus_tag="ACHKJF_000963"
     CDS             45609..46616
                     /gene="yggM"
                     /locus_tag="ACHKJF_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP0948367.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSNDDLYTWVGQLADYELLEQTWTKDKAVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREDAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(46854..47990)
                     /gene="hemW"
                     /locus_tag="ACHKJF_000964"
     CDS             complement(46854..47990)
                     /gene="hemW"
                     /locus_tag="ACHKJF_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP0948368.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMHTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPAQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(47983..48576)
                     /gene="rdgB"
                     /locus_tag="ACHKJF_000965"
     CDS             complement(47983..48576)
                     /gene="rdgB"
                     /locus_tag="ACHKJF_000965"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP0948369.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(48584..48874)
                     /gene="yggU"
                     /locus_tag="ACHKJF_000966"
     CDS             complement(48584..48874)
                     /gene="yggU"
                     /locus_tag="ACHKJF_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP0948370.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(48871..49437)
                     /gene="yggT"
                     /locus_tag="ACHKJF_000967"
     CDS             complement(48871..49437)
                     /gene="yggT"
                     /locus_tag="ACHKJF_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP0948371.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(49455..50159)
                     /gene="yggS"
                     /locus_tag="ACHKJF_000968"
     CDS             complement(49455..50159)
                     /gene="yggS"
                     /locus_tag="ACHKJF_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP0948372.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            50177..51157
                     /gene="yggR"
                     /locus_tag="ACHKJF_000969"
     CDS             50177..51157
                     /gene="yggR"
                     /locus_tag="ACHKJF_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP0948373.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(51333..51749)
                     /gene="ruvX"
                     /locus_tag="ACHKJF_000970"
     CDS             complement(51333..51749)
                     /gene="ruvX"
                     /locus_tag="ACHKJF_000970"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP0948374.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(51749..52312)
                     /gene="yqgE"
                     /locus_tag="ACHKJF_000971"
     CDS             complement(51749..52312)
                     /gene="yqgE"
                     /locus_tag="ACHKJF_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP0948375.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(52421..53371)
                     /gene="gshB"
                     /locus_tag="ACHKJF_000972"
     CDS             complement(52421..53371)
                     /gene="gshB"
                     /locus_tag="ACHKJF_000972"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP0948376.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(53384..54115)
                     /gene="rsmE"
                     /locus_tag="ACHKJF_000973"
     CDS             complement(53384..54115)
                     /gene="rsmE"
                     /locus_tag="ACHKJF_000973"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP0948377.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(54195..54902)
                     /gene="endA"
                     /locus_tag="ACHKJF_000974"
     CDS             complement(54195..54902)
                     /gene="endA"
                     /locus_tag="ACHKJF_000974"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP0948378.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAAGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(54997..55494)
                     /gene="yggI"
                     /locus_tag="ACHKJF_000975"
     CDS             complement(54997..55494)
                     /gene="yggI"
                     /locus_tag="ACHKJF_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP0948379.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(55571..56965)
                     /gene="galP"
                     /locus_tag="ACHKJF_000976"
     CDS             complement(55571..56965)
                     /gene="galP"
                     /locus_tag="ACHKJF_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP0948380.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT        13915 a        14159 c        15256 g        13786 t
ORIGIN      
        1 gcgtcgcatc aggcattgtg cacgattgcc ggatgcggcg tgaacgcctt atccggcctg
       61 gatggcttgc tgcgacgaac accaaccctt acccctgacg cttatcttcc gtattcgtct
      121 cgaaatcact ggcgtcatgg cgctcatgca actgctcatt cagcggtccg ttggtgcggt
      181 taacaatacg cccacgtttc accgccggac gttcgcctac ttctttcgcc cagcgttgta
      241 catgcttata actgcccgca tcaagaaact cagcggcatc atacacacca cctaacacca
      301 cgttgccaaa ccacggccaa atcgccatat ccgcaatggt gtactcatcg cccgcaacaa
      361 acttatgctg cgccagttgc ttatccagca cgtcgagcag acgtttggct tccatggtaa
      421 agcggttgat ggcgtactca atctttaccg gtgcgtaatg gtaaaagtga ccaaaaccac
      481 cgccgaggaa cggtgccgcg ccctgtaacc agaacagcca gttcatcgtt tcagtacgct
      541 ttgccaaatc ctgcggcagg aagtagccaa atttctccgc cagataaagc aggatcgaac
      601 cagattcaaa cacgcggatc ggcggattat gcgtatgatc gcgcagcgcc gggatcttcg
      661 agtttgggtt cacttcgaca aagccgctgg agaattgatc gccatcgcca atacgaatca
      721 gccaggcgtc gtactctgca ccagtaacgc ccagcgccag cagctcctca agcataatcg
      781 ttactttctg accgttcggc gttcccagcg aataaagttg caatgggtgt ttgccaacgg
      841 gcagcgtttt ttcatgcgtc ggaccagaaa ccgggcgatt gatattggcg aacgcgccgc
      901 cagcggattt atcccacgtc cagactttcg cgggctgata agtattgtct gtcatagtga
      961 gttgccttct gagtggtagt gttgaagcag tgtagcaggt cacttgctgt acagaatgtg
     1021 aattttttaa ccctttgcgc cagccgctgc gatgaggtgt atgattagtc tgacctctgt
     1081 ccggttaatg tcgctgagca ggtaatcaga gcacaacaat tacgccaact gcttaggttg
     1141 tattgttaaa ggtaaagtga tgagcaaagg aacgaccagc caggatgccc cgttcgggac
     1201 attattgggc tacgccccag gtggggtagc aatctactct tcagattaca gttctctcga
     1261 tccgcaggaa tacgaagatg acgccgtatt ccgtagctat atcgacgacg aatatatggg
     1321 ccacaagtgg caatgcgttg aatttgctcg ccgttttctc tttctgaatt acggtgtggt
     1381 ctttactgac gtgggtatgg cgtgggagat tttctcgctg cgcttcctgc gagaagtggt
     1441 taatgacaac atcctgccat tgcaggcatt tcctaacggc tcgccgcgtg cgccggtcgc
     1501 gggtgcgctt cttatctggg ataaaggcgg tgaatttaaa gacactggcc atgtcgccat
     1561 cattacccaa ttgcatggca acaaagtccg tattgcggaa cagaacgtga ttcattcccc
     1621 gttgccgcaa gggcaacagt ggacgcgtga gctggagatg gtggtcgaaa acggctgcta
     1681 taccctgaaa gacacttttg atgacaccac cattctgggc tggatgatcc agacggaaga
     1741 tactgaatac agcttaccgc agccggaaat tgcaggcgag ctgctgaaaa tcagcggagc
     1801 gcgcctggaa aacaaaggcc agtttgacgg taaatggctg gatgaaaaag atccgctgca
     1861 aaacgcctat gtgcaggcca acggtcaggt gatcaatcag gatccttatc attactacac
     1921 cattaccgag agtgccgagc aggagctaat taaagccacc aacgagctgc acctgatgta
     1981 tcttcacgca accgacaagg tgctgaaaga tgacaacctg ctggcgctgt tcgacatccc
     2041 gaaaatcctc tggccacgtt tgcgtctctc ctggcagcgt cgccgtcacc atatgatcac
     2101 tggtcgtatg gatttctgca tggatgagcg tggcctgaag gtttacgaat acaacgccga
     2161 ctctgcctca tgtcataccg aagcgggctt gatcctcgaa cgttgggcgg agcagggcta
     2221 taaaggcaac ggcttcaatc cggcggaagg gctgattaac gaactggctg gtgcctggaa
     2281 acacagtcgt gcacgtccgt ttgtccatat catgcaggac aaagatatcg aggaaaacta
     2341 tcacgcgcag tttatggagc aggcgctgca ccaggcgggc tttgaaacgc gtatcttgcg
     2401 cgggctggat gaactgggct gggatgctgc cgggcaactg attgatgggg aaggacggct
     2461 ggttaactgc gtgtggaaaa cctgggcgtg ggaaaccgcg tttgatcaga ttcgtgaagt
     2521 tagcgaccgt gagtttgctg cggtgccaat ccgtaccggt catccgcaaa acgaagtgcg
     2581 tcttatcgac gtattgctgc gcccggaagt gctggtcttt gagccgctgt ggacggtgat
     2641 ccccggcaac aaagcgattc tgccgatcct ctggtcgctg ttcccgcacc atcgttacct
     2701 gctggatacc gatttcactg ttaatgatga actggtgaaa acaggttacg cagtgaaacc
     2761 gatcgccggt cgctgtggca gcaatatcga cctcgtcagc catcatgaag aggtgctgga
     2821 caaaaccagc ggtaaatttg ccgagcagaa aaacatctat cagcaactgt ggtgtttgcc
     2881 gaaagtggac ggtaaataca ttcaggtatg taccttcacc gttggcggca actacggtgg
     2941 gacgtgtttg cgcggtgatg aatcactggt catcaaaaaa gagagtgata ttgaaccgtt
     3001 aattgtggtg aaaaagtaat acatgttgta gcaaccccgt actgattatg aagaataatc
     3061 agtacgggga ttaatcaaaa tattcaatat ttatatattc caatatttat tcatttcata
     3121 tgtgaatata ttaaccagtg gaatacctgt gttttgatta tttctaaaga ttttgaatga
     3181 atgcttattg tctgatacac aagaaataac actcttttta tcgttaaaag atgatatttc
     3241 actttgccca cgccgtgaaa gtcactgata atgcgtccgt tcgtaaattc aaaatggcgt
     3301 aatctaatat atgctaaatt tatttgttgg ccttgatata tatacggggc ttttgttatt
     3361 acttgctctg gcatttgtgt tgttctacga agcaatcaat ggttttcacg acacggcgaa
     3421 cgcggtggca accgttattt atactcgtgc catgcagccg catcttgcgg tagtaatggc
     3481 ggcgtttttt aacttttttg gtgtgttgct tggcgggctt agcgtcgcct atgccatcgt
     3541 ccatatgttg ccaaccgatt tgttgctgaa tatggggtca actcacggtc tggcgatggt
     3601 cttttccatg ctgctggcgg cgattatctg gaacctggga acgtggttct ttggtttacc
     3661 ggcctccagt tcgcacacct tgattggtgc gattattggc atcggtttaa ccaatgcgct
     3721 gttaaccggc tcatcggtga tggatgcgtt aaacctgcgt gaagtgacca agattttctc
     3781 ctcactgatt gtttccccta tcgtcggcct ggtcattgcg ggggggctga tatttctgct
     3841 gcgacgctac tggagcggga cgaaaaagcg tgaccgtatt caccgcattc cggaagatcg
     3901 caaaaagaaa aaaggcaaac gtaaaccgcc attctggacg cgtattgcgc tgattgtttc
     3961 cgctgcgggc gtggcgtttt cgcacggcgc gaacgacgga caaaaaggga tcggcctggt
     4021 gatgctggta ctggtgggga ttgcccctgc tggcttcgtc gtcaatatga acgcgtccgg
     4081 ctatgaaatt acccgtaccc gcgatgccgt caccaacttc gaacactacc tgcaacagca
     4141 tcctgaactg ccgcagaagt tgattgcgat ggaacctcca ttgcctgcag catcgactga
     4201 tggcacgcaa gtaacagagt ttcactgtca tccggcaaat acctttgatg cgattgcgcg
     4261 cgttaaaacg atgctgccag gcaatatgga aagttacgag ccgttaagcg tgagtcagcg
     4321 cagccagctg cgccgcatta tgctgtgcat ctctgatacc tccgcgaagc tggcgaaact
     4381 gccaggcgtc agtaaagaag accagaacct gctgaaaaaa ctgcgcagcg atatgttaag
     4441 caccattgag tacgctccgg tgtggatcat catggcggta gcactggcgc tcggcattgg
     4501 caccatgatt ggctggcgtc gtgtagcgat gaccatcggt gagaagattg gtaagcgcgg
     4561 catgacgtat gcgcaaggca tggcggcaca aatgacggcg gcagtgtcta tcggtcttgc
     4621 cagttatatt gggatgcccg tctccacaac acacgtcctc tcgtctgcag ttgcagggac
     4681 gatggtggtg gacggcggtg ggttacagcg taaaacggta accagcatcc tgatggcgtg
     4741 ggtatttact ttaccggcgg caatttttct ttctggtggg ctgtactgga tagcattgca
     4801 gttgatttaa ccttcctgaa aatgcccggt cctggcgatc gggcatttcc atttttgact
     4861 agtgataacc acgcgcggtc ataaaatccg taatcgcttt ttctgcatca accaacacct
     4921 gttccagtgg ctgattggca tcgatatcaa ccagttgtgc accaccaaag gttaactgtg
     4981 gcgttatggc tatctttctt gccagcgatt cccttttgtg gtcaggctta cgcgcgcagg
     5041 ccacgtctag atcgacattg agtttgatga ccagatcagg cttatggctc gccatccagt
     5101 gaaacgcttt acgttcctgg cttgccagcc atgagacaaa acgaccacct tcaacgttag
     5161 gtgggaacac cgtaccatcg taagcgccag gaatttggtc ctgaggataa cggtcggtta
     5221 gaacaattaa cccgcgacga cgacaggcaa gcatatgacg aaagcgcagt aagcgacggg
     5281 cgacaaacgc tgtaattacc agcgccggaa ctggtcctgg caattttttt gctgttttca
     5341 cctgatttcg ttcaattgtt ttatgtaagg attttcccat caacggtaat tttgtcactg
     5401 cacgaccgac atttccggcc tgttttccta aatgaactct ttcggcagca ccatattttt
     5461 cgacaacggt aataagatgt tcacacaccg ttgacttgcc tgaaccatcg ctaccaataa
     5521 cggcaattaa tggaggtgtt attgactgca tgaatatatc ctttaattta aatatccatt
     5581 aaaaatattt atttggttaa tatgttttta tgaaagcgta attcaggtca atgtcacaat
     5641 taaccattgt cacaataagg ttgaacggat attctgagca cggaccacgt aatattcaat
     5701 acattattta ctgccgggtt tttcgtgaat caacgtaata tgtcaataat taattccacg
     5761 ccagtgcatg ttattgccat tgtaggatgt gatggttcag gtaaatcgac cctcacggca
     5821 agcctggtaa atgaactggc agcaagaatg ccaacagaac acatttatct cgggcaatcg
     5881 tccgggcgaa ttggcgaatg gatttcacag ctccctgtta ttggcgcacc ttttgggcgt
     5941 tatctgcgaa gtaaagcggc acatgtgcac gaaaagccct caacaccgcc tggcaatatt
     6001 actgcactgg ttatctatct gctttcctgc tggcgggcgt acaagtttcg caaaatgttg
     6061 tgtaaaagcc agcaaggctt tctgctcatc accgaccgct acccgcaggt tgaagtgccg
     6121 gggtttcgct ttgatggccc gcaattggca aaaaccacgg gcggtaacgg ttggataaaa
     6181 atgttaaggc agcgcgagct gaagctgtac caatggatgg catcttattt gcccgtattg
     6241 ttgattcgtc ttggcattga tgaacaaacg gcgtttgcgc gtaaacctga ccaccaactg
     6301 gcagcgttac aggaaaaaat cgccgttacg ccgcaactga cattcaatgg tgcaaagatc
     6361 cttgaagtgg atggtcggca accagccgat gaaattctgc aagcgtcact acgcgcaatt
     6421 cacgccgcgc tttcccgatc accaaaatgg ataggggatg atttaacgaa tggatgcact
     6481 acaaacacaa actgttatta gtacaaccgc gccgcagccc agctacattc cggggctaat
     6541 tgcggtggtc gggtgtgatg gcaccggtaa atccacactg accaccgacc tggtgaaatc
     6601 gctgcaacaa cactggcaaa ccgagcggcg ctatctgggg ctgctctccg gcgaagacgg
     6661 cgacaaaatc aaacgattgc cgttggttgg cgtctggctg gaacggcgac tggcggccaa
     6721 atcctcgaaa acccaaagca tgaaaaccaa atctccggcg ctatgggcgg cggtgattat
     6781 gtactgcttc tcgctgcgaa gaatggcgaa tctacgcaag gttcagcgac tggcgcaaag
     6841 tggcgttctg gtggtcagcg atcgcttccc gcaggctgaa atttcgggct tttattatga
     6901 tggaccgggg attggcgtcg aacgtgcgac cgggaaaatc agcatgtttc tggcgcagcg
     6961 cgaacggcgt ttataccaac aaatggcgca atatcgcccg gaattaatta ttcgcctggg
     7021 cattgatatt gagactgcca tctcccgcaa gcctgaccat gactatgccg agctgcagga
     7081 caaaatcggt gtcatgtcga agattggcta taacggcaca aaaattcttg agattgattc
     7141 cagagcgccc tacagcgaag tgctggaaca ggcacagaag gcggtttctc tggttgccat
     7201 cgtttctgac cgccgaagtt taacttaagg ctggcgggat actttttact atcaacgata
     7261 aggtcaggct gaattggcgg gttttaacat caaacattgg tttgcagatg gcgcgtttcg
     7321 caccattatt cgcaatagcg cctggttagg ctccagtaat gtcgtgagcg ccttgctggg
     7381 gctgttggcg ctctcgtgtg ccggtaaagg gatgacgccc gccatgtttg gcgtactggt
     7441 gattgtgcaa tcgtacgcca agtcgatcag cgattttatt aagtttcaga catggcaact
     7501 ggtggttcag tacggaacac cagcattaac caacaataat ccgcagcaat tccgcaatgt
     7561 cgtctcattt tccttctcgc tggatatcgt cagcggcgcg gtggcgattg tcggtggcat
     7621 tgccttactg ccattccttt cccattcatt aggtctggat gaccaaagct tttggctggc
     7681 ggcgctgtat tgcacgctca ttccttcaat ggcttcctcc acgccgaccg gcattctgcg
     7741 tgcggtagat cgcttcgatt taattgctgt acagcaggcg acgaaacctt ttctgcgcgc
     7801 agcggggagc gtcgtagcct ggtattttga ctttggtttt gcgggttttg ttattgcctg
     7861 gtacgtgtcg aatctggttg gcggcaccat gtactggtgg tttgcagcgc gcgaattacg
     7921 ccgcagaaat atccataatg ccttcaaatt gaatctgttt gagtctgccc gacacatcaa
     7981 aggcgcgtgg agttttgtct ggtcaaccaa cattgcccac tccatctggt cggcgcgtaa
     8041 ctcgtgcagc acagtgctgg tggggatcgt gttaggaccc gctgccgccg ggttatttaa
     8101 aatcgccatg acattcttcg acgccgccgg aacgccagcg ggtttgctgg gtaaaagttt
     8161 ttacccagag gtgatgcgtt tagatccgcg caccaccaga ccgtggttgt taggtgtgaa
     8221 gtccggttta ctggcgggcg gaatcggtat tctggtggcg ctcgcggtgt ttatcgtcgg
     8281 caagccgctc atttcgctgg tgtttggcgt taagtatctc gaagcgtatg acctgattca
     8341 ggtgatgctg ggcgcaattg tgatttcgat gctgggcttc cctcaggagt cattgctatt
     8401 gatggcggga aaacagcgcg cttttctcgt ggcgcaaacc atcgcctcaa tcggctacat
     8461 cgtattgctg ttcatgttct gtcatctgtt tggtgtgctg ggagcagcgt ttgcttactt
     8521 cggtgggcaa tgtctggacg tggcgctctc gttaattcca accttaaagg cattttttca
     8581 gcgccattcc ttgctttata acgcagccgg agagaaatcc tgatgatgag aaaatatttc
     8641 ccgcttgagg cgagcgagcg tctatttgtt gcgattgaag aagatgatgt cgttgatgca
     8701 caggttagtt tgccgccgac tattgcacta agttgtacca ccgaaatcat tcatgataat
     8761 tacgcgttat gcctgcaatt ctggctcaat ggcgttgatc gtcaggaatt actgcgttta
     8821 gttcgcaaac aagccaaagg cgatgagctg actgctgatg agcgcaaaca atttaaatac
     8881 atgcgagcgc gctacaagca cctgcgtttt gcgcaacggc tgtatttaaa gaagcatcag
     8941 gccggatttt tattcggcaa aacgaccgtt tttctggggc gttttcagga cggctttcgt
     9001 aacggtaaga aaaatattgt gtcgtattac ggcaacctgt tacggattta tttaagctct
     9061 ccggtctggt cgttggtgaa ctattcatta cgccatagcc agttagaaag cgtaagcagt
     9121 tttattgcct atcggcaaaa gcaaatgcat accctgaaag agattattgc caaaccgcga
     9181 ctaaccggca gggaatttca tgatgtgcga aaaatcatca gccaacaggt ttcttattac
     9241 gacaccttaa gatcgcttga tcctgaaaat aaagaggcgt tacagatttc ccgtttcctc
     9301 gccgccatta atggcctgat gggagataaa cacgacgaca tggtggcgga tgatatggaa
     9361 aaccgccagt cgtatgatgc accgttggcg ctggatagcg atattcgtca gcgcttagag
     9421 ctgctgattt cgcgttttcc gctatagccg gagcaatgat gtcatctcgt ttgattattg
     9481 cgttgattat catgctctta gccccgggtg tgcaggcaca caatttcgtc actggcaaaa
     9541 cggttacccc tgtttatatc caggaaggcg gcgagctgct tctgaatagc gacgacgaaa
     9601 ttcactacca aaagtggaac agcacacaat tagcgggaaa agtccgcatt attcagtata
     9661 tcgccgggcg taagtcggcg aagaaaaaga actcactgtt aattaaagcg gttgaagcgg
     9721 caaactttcc gcaggatcgt tttcagccca ccaccattgt gaacaccgat gatgccatct
     9781 ttggtaccgg ctattttgtc gttggcaaaa tcgaaaaaaa taaacgccgc tatccgtggg
     9841 cgcaattcgt tattgacggc aatggacagg gacgcgttgc ctggcgcttg ccggagcaga
     9901 gttcaaccat tctggtgctg aataaagccg ggcaaattca gtgggcaaag gacggcagcc
     9961 tgacgccgga agaggtagat catgtcattg cgttggcgca gaaactgatc aatgaataaa
    10021 aaactcaaag gattgagggc gttagtgaga tgaagctggt tgaacactac atcatgcgtg
    10081 gtacgcgccg tctggtgctg attatcgtcg gttttctgat ctttattttc gccagctact
    10141 ccgcacagcg ttatctgacc gaagcggcaa atgggacatt agctcttgat gttgtgctgg
    10201 atatcgtttt ctacaaagta ctgattgcac tggagatgtt gttacctgtt ggtctgtatg
    10261 tgtcagttgg cgtgacgcta gggcagatgt ataccgactc ggaaattacc gctatctctg
    10321 cggcgggggg cagtccggga cgcttgtaca aagccgttct ttatctggcg ataccgctaa
    10381 gtatttttgt cacccttctg tcgatgtatg gtcgtccgtg ggcttatacg cagatttatc
    10441 aactggagca gcagtcgcag tcggagctgg atgttcgcca gttgcgggca aagaaattta
    10501 acactaacga taacggacga atgatccttt cgcagacggt tgatcaggat aataatcgcc
    10561 tgactgacgc gctgatttat acttcttctg ccaatcgaac ccgcattttc cgcgcccgtt
    10621 cggttgatgt ggttgaccca tcacccgaga aaccgaccgt tatgttgcac aacggcactg
    10681 cctatcttct cgatcatcag gggcgtgacg acaacgaaca gatctaccgt aatctgcaat
    10741 tacatctgaa accgctggat caaagcccta acgtcaaacg caaagcgaaa tcggtcacgg
    10801 agctggcgcg ctccgccttt cctgccgatc atgccgaatt gcaatggcga caaagccgtg
    10861 gcctgacagc attgttgatg gcgctgctgg ccatttcatt aagtcggata aaaccgcggc
    10921 aagggcgatt ttcaacatta ttgccactga cgttgctgtt tattgccatt ttttatggcg
    10981 gcgacgtctg ccgttcgctg gtggctaacg gcgcgattcc cctcattcct ggtttgtggt
    11041 tagtacccgg actcatgcta atgggcctgc tgatgctggt cgcacgcgac ttctctttgc
    11101 tgcagaaatt ttcccgatga atgttttcag tcgctattta atccgtcatc tctttctcgg
    11161 ttttgccgcc gccgcagggt tattgctgcc gctttttact accttcaacc tgattaacga
    11221 actggatgat gtcagcccgg gcggttatcg ctggactcag gcggtgctgg tggtgctgat
    11281 gaccttaccg cgtacactgg tcgaactttc gccgtttatc gcattattgg gagggattgt
    11341 tggcctgggg cagttatcga aaaacagtga gcttaccgcc attcgcagca ctgggttttc
    11401 tatcttccgt attgcactgg tggcgctggt tgccgggata ttgtggactg tttcgttagg
    11461 cgcgatagat gagtgggtag cgtcgccatt gcagcaacag gcgctgcaaa tcaaatcgac
    11521 cgccactgcg ttgggggagg acgatgatat taccggcaat atgctgtggg ccaggcgcgg
    11581 taatgaattt gtgacggtga aatcgctgaa tgaacagggc cagcctgtgg gcgtggagat
    11641 atttcattat cgcgacgatc tctcgctcga atcctacatt tatgcacgca gtgccaccat
    11701 tgaagacgac aaaacgtgga ttctgcatgg tgtgaatcat aaaaaatggc tgaatggcaa
    11761 agaaacgctg gaaatattag ataatcttgc ctggcaatcg acattcacca gtatgaatct
    11821 tgaagaactg tcgatgccgg gcaatagctt ttccgttcgt cagctcaacc attacatcaa
    11881 ttacttgcag gaaaccggac aaccgagcag tgagtatcgc ctggcattgt gggaaaagct
    11941 ggggcatccc attttaaccc tggcgatgat tttgctggca gtgcctttta cctttagcgc
    12001 cccgcgctcg ccggggatgg gcagccgtct tgctgtaggc gtcatcgttg gcttacttac
    12061 ctggataagc taccaaataa tggtcaatct gggattatta tttgcgctga gtgcgcccgt
    12121 tgctgcactc ggtttaccta tggcatttgt gctggcggcg ttgattctgg tgtatctatt
    12181 tgataggcaa cattaaaccc gccgtggcgc aacaccacgg cgggctcaca ttactgcttc
    12241 agagtagcaa atgcctgaat gatggcatcg atttgctcac gactgtgggc agcgtttacg
    12301 ctacagcgca gcagcgtgat gcctgcaggc gcggcaggtg gcaggataag attcacataa
    12361 acgcccagcg aaataagctc gcgccagatg cgtaaacctt cctctttcga accgattatt
    12421 accggcacta ccgggctaat atgtgaactt aactcataac ccagtttttg cagaccgtca
    12481 tacagatggt tggcgttatc catcagcttt tgccgcagct ctggttgtgt ggcaatagtt
    12541 cttaacgacg agcgcacggt cgcaatgcag gacggagaag gagaggcggt aaaaatatac
    12601 gggcggctgc cgtagcgcag cacttccatc gcttccgaac cgacagcaaa accgccaatc
    12661 gaggccagac ttttactgaa agtaccaacg ataatatcca catcatcttc aacgccgaca
    12721 gcttcagcca gtccgcgccc cgttgcgcct aacacgccaa acgaatgcgc ttcatcgaca
    12781 atcaaataac caccgagacg acgtttgata tcaacaattt ccgccagcgg cgcaacgtca
    12841 ccgagcatgc tgtagatgcc ctcaacgatg ataatcgcct ctttcgcccg ttcaccgagg
    12901 cgaaccatgc gccgttccag atctttagca tcgttatggc ggaaacgaat aatttccgcg
    12961 ccaccgagag aacaggcgtc ataaatactg gcgtggctgt cggcatccag taacaccacg
    13021 gcgttatggt cggcaagtgc gctgatgacg cctaaattag cggtataacc ggttgaaaaa
    13081 acgatagcgg tagggcgctt gaaaaactcg gcaatttctt tctctaacgc caggtgcggc
    13141 gcataactgc cgtttgccat acgagaaccg gtggtgccgg tgccttgggc cgctaacgct
    13201 gcctgacctt cgctgatggc atcatgatta aaggttaaac cgagataatt attggtcccg
    13261 gccagaatca cttttttatt gccgatccgg ccttcggtgg cggaatacac ttcatcaata
    13321 caggtgccaa acggtgttaa cccgttgtca ctaaattgca gacgctcgcc agcaaggcgc
    13381 gcatatttat cgtatagccc cattatttat tctccagcca tggaagtaga gtttccatta
    13441 attgcgcagg ggttttgaca tccagcagaa tattaatagg aatggaaata tcgaatctgt
    13501 cttccagcat cattaagaga tccatcactt tgatcgactc caggccaaga tcgttgacga
    13561 gatcgctgtc gggcttaatt tccacaccgt tttcgaccaa atcctgtaaa caggaaagga
    13621 tgtaatccat tactatttca cgatttacca tagaaacgtt aacacacctt taaacaagac
    13681 aatcaaaata gcccgttgcg taatgcttgt tccaggctaa tcccgggaaa ccaattaata
    13741 tcttcagaaa tacggttatt acttgccgac cagtcggcgt gggttaattc acgaattttc
    13801 gagcgggtca gcataggttc tttacctgcc aggcgactca acgcggtact gatatccgca
    13861 aggcaggtga gtaccggcag aggaatacca accattcgca cggaaccgca acggacgttg
    13921 gcggcaagtt gctgtatgcg ttgccagtca tagccgccag cgacgccatc gcataattca
    13981 taggtttgcg tctgtacagt ttcggcgctt aaccactgac ccactgcttg cgcaaaatcg
    14041 gtgacgtgca ggaaagagag ctgtgtatct ggtgcaccaa gtcgtggcag caggccgcgc
    14101 agcatccagt caaacagcgg ttttaactct ttatcgccgg gaccatacac ggctgtcggg
    14161 cgaaaaacgc ccagcgtaat ttcgtcagcc attgcagtca gccgttgttc ggcgatgtgt
    14221 ttggaatttg cgtaccagga gagctcggga tggcgcgccg ccagcgaaga gataaacaga
    14281 aaacgttggc aaaagcagct ctcttttgct gcttgcatca ggcgcaggct gccgtcaacg
    14341 ttacagcggg tgaaaatctc ttctttgtgc ccgcgcactt gcccggcgca atggaccact
    14401 gcgctggctc cggcaaccag ctcgctaagt gaatgagtat cttccagcga accgcgcacc
    14461 caggtgagat tatcattgac gtgagcgcgg gcagtacgcg tcaatgcgcg aacgtgaaag
    14521 ccgcgggcga gcaggttatc gataatatat ttaccgataa acccggtagc gcccgtcacc
    14581 gcgacagttt gattcatgcc agggattcct gcacatgaag gcttgcagca taagccttct
    14641 gataacgttt tttcgcttcc gcacgggcag gcttgccgga ggatgttcgg ggaatactgt
    14701 ggggcggcaa cagatcgata gccgcggtta cgccaaattc gctttggatc cgtgccgcca
    14761 gcgcgtggat aagctgcccg cgacgttctt cgtcgctgat ccgacactgg atctgcaaaa
    14821 tgattttttc ctggacggta acaaaagcaa tcgcatcgcc agaatgaatt tccggttctt
    14881 gttccgcaat atattcaata tcctgcggcc agatattacg gccacgaata ataatcagat
    14941 ctttaatacg tccggtgaca tacagataac cgtccagcag ataaccgagg tcgccggtgt
    15001 ctaaccagcc cgtcgcggca atctcgtctt gcgaaacctg gtcgccaaag taaccgctca
    15061 tcaggctggg gccggagatg caaatatggc ctaccacacg ttccgcgacc ggcatacctg
    15121 cttcattgcg gatttcaatg ccatgttccg gcaacgcttt gccgcagttg acgaaagtcg
    15181 atacggcgcg tgtttcagca cccggcgcga cggctttgcc ctgatattct aggatgtcgc
    15241 gatccacttc gttaaccaca accccagagc cttcatcaga gaagctgaca gccagcgcat
    15301 tttctgccag tccgtagcac ggcatgaacg ttttatcgtc aaagttaacc tgacggaaac
    15361 attcagcgaa ttgatggagt tgttctgcgg agatcggctc agcaccaata ccagcgacgc
    15421 gccagcagga aagatccagt tcagcgagat ctttttcatt cacgcggcgt tggcacaatt
    15481 catagccaaa cggcggcgca acggaaacgg tgccgcgatt tttactgatc aatttaagcc
    15541 attgcaaagg acgcatggca aaatcctgag tgcgcaaata atctactgaa agttgcgtgg
    15601 cgacgggggt caggagaaag ccgaccagtc ccatatcatg gtagaaaggc agccaggaga
    15661 cgcagcggtc gccagggcgt aatttaatgc catcgtggct tatagcacgt agattagcca
    15721 tcacttcgcg atgggtgata atgacgccac ggggaaaacg ggtgctgccg gaggtgtact
    15781 ggaggtaggc gatatcgttc ggaactggac gctggagcgc aacatcggct tccggtaatg
    15841 ccttaaacca ggcgtggctt aaaacatgta attcggggtt gttatgcgtc gcggcattga
    15901 ccagtggcaa ccactcatca ccagtgataa tggctgcggg ctggcaactt gccagtaaac
    15961 cctggagttt ggcgctccag gaatcccgct gaccgacgcc cattggaatc gccaacggga
    16021 cggcgactaa gccagcatac tgacaggcaa aaaaagcctc tacaaacccg ctactcgttt
    16081 cggcaatcag cgccacgcgt tcgccttttt tcaggttcag cgataacaac cgcttcgcac
    16141 cagcttcggc acgcgttttt aatgtctgat attccagttg atcttcaagt tggcaacgtc
    16201 tgtcataaaa attcattccg gcgctactca gagcggcgta gtccaaagca tcaaccagcg
    16261 ttggaaaatc ggcatagcgc ataggtaggg aatgcgtaaa gattttatta gacatataca
    16321 ccacgaaaaa attggatatc aaaaataaaa tgacaataat gcggtggatt atataggcat
    16381 aaaagataaa tgagatagta ttttcatcgg tgtgaaaatg aacattgatt taaataatga
    16441 gtttaagttt tttgttttta ttgtgaatga tatttaattt atgaatatat taagaagaag
    16501 ccttgtccgg tcgccttaaa tcgacaaaag gcattgaggc gcttctgtgt agtctctaac
    16561 cttatcataa tcattaataa atactattta ttctttgttt gtattcattg ggttgattat
    16621 taaggtgtcg ttcgttgctg ttgcaacctg tttttcaccg ttaattattt gttatccaca
    16681 gagatatgga atgcgatctg ctgatgatta aaattgaaaa agtaattaat aaaaatgacc
    16741 ttaaggcatt tatcgctttt ccgtcatcac tttatcccga cgatccaaac tggatacccc
    16801 ctttattcat agaacgcaac gagcatttgt ctgcgaaaaa tccggggacg gaccatatca
    16861 tctggcaggc gtgggtggca aaaaaagcgg ggcagatagt tgggcgtatt accgcgcaaa
    16921 tagatacctt gcaccgtgag cgttacggtg aagataccgg tcatttcggc atgattgacg
    16981 ccattgatga tccgcaggtt tttgccgcgt tgtttggcgc agcggaagcg tggttgaagt
    17041 cacaaggtgc aagtaagatc agcggtcctt tcagcctgaa tatcaatcag gaaagcggat
    17101 tactgattga aggttttgac acaccaccct gtgcgatgat gccacacggc aaaccgtggt
    17161 atgccgcgca tattgaacaa ctgggttatc acaaaggcat tgatttactg gcgtggtgga
    17221 tgcagcgaac cgatctcact ttctctccgg cgctaaaaaa actgatggat caggtgcgca
    17281 aaaaggtgac cattcgctgc atcaatcgtc agcggtttgc cgaagaaatg cagatcctgc
    17341 gtgagatttt caattctggc tggcagcaca actggggatt tgtgccgttt accgaacatg
    17401 aattcgcgac catgggcgat caacttaaat atctggtgcc ggacgatatg atctatatcg
    17461 ctgagattga ttctgcaccc tgcgcgttta ttgtcggctt gccgaacatc aacgaggcga
    17521 ttgccgatct gaacggatcg ctctttccct tcggctgggc aaaattgctg tggcgtttga
    17581 aagtcagcgg tgtgcgaact gcgcgagtac cgctgatggg cgtgcgcgac gagtatcagt
    17641 tcagccgcat cggcccggtg attgcgctgt tattgattga agccttacgc gatccgtttg
    17701 cccgccggaa gatcgatgcg ctggagatgt catggatcct cgaaaccaat acaggcatga
    17761 ataacatgct ggaacgcatt ggggcggagc cgtacaagcg ttaccgattg tatgaaaagc
    17821 agatttaata ccagctaact caggttcatc tccagcggca ccgccgagcg aatcaaatgg
    17881 tggccttcca gctcgatatc gtggagattc tttttcacgg tccgcacatg atcgcgtgct
    17941 gcgcgctggg cgacgtgcgg cagccgctgc aacaccgcgt tgtagatcct cgcatgctgg
    18001 cgatcgatct gctttttttg ctgtggtcga tggtagagat tatttacgct ggcaaacact
    18061 gaattaaaca tcagatcggt caatgattgc agcgtaaaca ccagcacctg attgtgagaa
    18121 gcctgacaaa tggcgagatg gaaagcgtga tccaactgcg catgttcgat cagcgaaatc
    18181 tctttgttgt tctcactggc ggcgagcatt ttttcataac agcgggttat cacaacaaaa
    18241 tcagcctgcg ttcccagcgt tgccgccagc cttgccgatt cgccctccag taatgcgcga
    18301 acgtcgagca gatcgtacag cgttcgcggc tgcgtactga acagatggat cagcgggctg
    18361 gtgtcctgca cccgattaag ccgtgcgaca cgagaatcgc gaccctgcgc cgtttcaata
    18421 atcccgcgcc cgcgcagcac ggtcagccct tcacgcagtg cggagcgtga gaagccgagc
    18481 ttttcacaca gtcgacgttc cgagggaagt ggctgaccga ccttcagtac gccgtcgata
    18541 attaaccgtt cgatactctc tgcaaccact tcgcaaatag ggcgacgttc atctttcatt
    18601 cccggacctc gtgcacaggt aggaccaatt tttctgttct taactcgcaa aacacgcaca
    18661 tcacgtaagt gattatgtta catcaattta acattaagtt aaccaagaca aggtcacaga
    18721 gctggaaaaa aaatggtctg accggtagct aaagagatag acgaaaacga aaagcccgct
    18781 taataactgt tcacagaagc agcgcgcaaa aatcagctgc cacacaacac aacaaagcga
    18841 agcctactca tgagcatctt gtacgaagag cgtcttgatg gcgctttacc cgatgtcgac
    18901 cgcacatcgg tactgatggc actgcgtgag catgtccctg gactggagat cctgcatacc
    18961 gatgaggaga tcattcctta cgagtgtgac gggttgagcg cgtatcgcac gcgtccatta
    19021 ctggttgttc tgcctaagca aatggaacag gtgacagcga ttctggctgt ctgccatcgc
    19081 ctgcgtgtac cggtggtgac ccgtggtgca ggcaccgggc tttctggtgg cgcgctgccg
    19141 ctggaaaaag gtgtgttgtt ggtgatggcg cgctttaaag agatcctcga cattaacccc
    19201 gttggtcgcc gcgcgcgcgt gcagcctgga gtgcgtaacc tggcgatctc ccaggccgtt
    19261 gcgccgcata atctctacta cgcaccggac ccttcctcac aaatagcctg ttcaattggc
    19321 ggcaatgtgg cggaaaatgc cggtggcgtc cactgcctga aatatggtct gaccgtacat
    19381 aacctgctga aaattgaagt gcaaacgctg gacggcgagg cgctgacgct gggatcggac
    19441 gcgctggatt cacctggttt tgacctgctg gcgctgttca ccggatcgga aggtatgctc
    19501 ggcgtgacca ccgaagtgac ggtaaaactg ctgccgaagc cgcccgtggc gcgggttctg
    19561 ttagccagct ttgactcggt agaaaaagcc ggacttgcag ttggtgacat catcgccaat
    19621 ggcattatcc ccggcgggct ggagatgatg gataacctgt cgatccgcgc ggcggaagat
    19681 tttattcatg ccggttatcc cgtcgacgcc gaagcgattt tgttatgcga gctggacggc
    19741 gtggagtctg acgtacagga agactgcgag cgggttaacg acatcttgtt gaaagcgggc
    19801 gcgactgacg tccgtctggc acaggacgaa gcagagcgcg tacgtttctg ggccggtcgc
    19861 aaaaatgcgt tcccggcggt aggacgtatc tccccggatt actactgcat ggatggcacc
    19921 atcccgcgtc gcgccctgcc tggcgtactg gaaggcattg cccgtttatc gcagcaatat
    19981 gatttacgtg ttgccaacgt ctttcatgcc ggagatggca acatgcaccc gttaatcctt
    20041 ttcgatgcca acgaacccgg tgaatttgcc cgcgcggaag agctgggtgg gaagatcctc
    20101 gaactctgcg ttgaagttgg cggcagcatc agtggggaac atggcatcgg gcgagaaaaa
    20161 atcaatcaaa tgtgcgccca gttcaacagc gatgaaatca cgaccttcca tgcggtcaag
    20221 gcggcgtttg accccgatgg cttgctgaac cctgggaaaa acattcccac gctacaccgc
    20281 tgtgctgaat ttggtgccat gcatgtgcat cacggtcatt tacctttccc tgaactggag
    20341 cgtttctgat gctacgcgag tgtgattaca gccaggcgct gctggagcag gtgaatcagg
    20401 caattagcga taaaacgccg ctggtgattc agggcagcaa tagcaaagcc tttttaggtc
    20461 gccctgtcac cgggcaaacg ctggatgtgc gttgtcatcg cggcattgtt aattacgacc
    20521 cgaccgagct ggtgataacc gcgcgtgccg gaacgccgct ggtggcgatt gaagcggcgc
    20581 tggagagcgc cgggcaaatg ctcccctgcg agccgccgca ttatggtgaa gaagccacct
    20641 ggggcgggat ggtcgcctgc gggctggcgg ggccgcgtcg cccgtggagc ggttcggtcc
    20701 gcgattttgt cctcggcacg cgcatcatta ccggggctgg aaaacatctg cgttttggtg
    20761 gcgaagtgat gaaaaacgtt gccggatacg atctctcacg gttaatggcc ggaagctacg
    20821 gttgtcttgg cgtgctcact gaaatctcga tgaaagtgtt accgcgaccg cgcgcctccc
    20881 tgagcctgcg tcgggaaatc agcctgcaag aagccatgag tgaaatcgcc gagtggcaac
    20941 tccagccatt acccattagt ggcttatgtt acttcgacaa tgcgttgtgg atccgccttg
    21001 agggcggcga aggatcggta aaagcggcgc gtgaactgct ggggggcgaa gaggttgcgg
    21061 gtcagttctg gcagcaattg cgtgaacaac aactgccgtt cttctcgtta ccaggtacct
    21121 tatggcgcat ttcattaccc agcgatgcgc cgatgatgga tttacccggc gagcaactga
    21181 tcgactgggg cggagcgtta cgctggctga aatcgacagc cgaggacaat caaatccatc
    21241 gcatcgcccg caacgctggc ggtcatgcga cccgctttag tgccggagat ggtggctttg
    21301 ccccgctatc ggctccttta ttccgctatc accagcagct taaacagcag ctcgaccctt
    21361 gcggcgtgtt taaccccggt cgcatgtacg cggaactttg aggagcaggc tatgcaaacc
    21421 cgattaaccg aagagatgcg gcagaacgcg cgcgcgctgg aagccgacag catcctgcgc
    21481 gcctgtgttc actgcggatt ttgtaccgca acctgcccaa cctatcagct tctgggcgat
    21541 gaactggacg ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga aggcaacgaa
    21601 gtcacgctta aaacacagga gcatctcgat cgctgcctca cttgccgcaa ttgtgaaacc
    21661 acctgtcctt ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga tattgtcgag
    21721 cagaaagtga aacgcccact gccggagcga atactgcgcg aaggattgcg ccaggtagtg
    21781 ccgcgtccgg cggtcttccg tgcgctgacg caggtagggc tggtgctgcg accgttttta
    21841 ccggaacagg tcagagcaaa actgcctgcc gaaacggtga aagctaaacc gcgtccgccg
    21901 ctgcgccata agcgtcgggt tttaatgctg gaaggctgcg cccagcctac gctttcgccc
    21961 aacaccaacg cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt catgtcagct
    22021 aacgaagcag gctgttgtgg cgcggtggac tatcatctta atgcgcagga gaaagggctg
    22081 gcgcgggcgc gcaataatat tgatgcctgg tggcccgcga ttgaagcagg tgccgaggca
    22141 attttgcaaa ccgccagcgg ctgcggtgcg tttgtcaaag agtacgggca gatgctgaaa
    22201 aacgatgcgc tgtatgccga taaagcgcgt caggtcagtg aactggcggt cgatttagtc
    22261 gagcttctgc gcgaggaacc gctggaaaaa ctggcaattc gcggcgataa aaaactggcc
    22321 ttccactgcc cgtgtaccct acaacatgcg caaaagctga acggcgaagt ggaaaaagtg
    22381 ttgcttcgtc ttggatttac cttaacggac gttcccgaca gccatctgtg ctgcggttca
    22441 gcgggaacat atgcgttaac gcatcccgat ctggcacgcc agctgcggga taacaaaatg
    22501 aatgcgctgg aaagcggcaa accggaaatg atcgtcaccg ccaacattgg ttgccagacg
    22561 catctggcga gcgccggtcg tacctctgtg cgtcactgga ttgaaattgt agaacaagcc
    22621 cttgaaaagg aataacaaaa tgaaaactaa agtcattctt agccagcaaa tggcgagtgc
    22681 aattattgcc gcaggtcagg aagaggcgca gaaaaataac tggtcggttt ccattgctgt
    22741 tgccgatgac ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg cgccgattgc
    22801 ggcttacatc tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg aaactaaggg
    22861 ctatgaagag atggtgaaca acggacgtac cgcgttcgtg actgcgccgt tactgacgtc
    22921 gctggaaggt ggcgtaccgg ttgttgttga tgggcaaatt attggtgccg tgggcgtttc
    22981 tggtttaacc ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg tgttggcgaa
    23041 ataagcgaaa acgaggagat aaacaatgag tcaaaccata acccagggcc gtttacgcat
    23101 tgacgccaat tttaaacgtt ttgtggatga agaagttttg ccgggcgtgg aactggacgc
    23161 tgccgcattc tggcacaatg ttgatgagat cgttcacgat ctggcaccag aaaatcgtca
    23221 gttgctggca gagcgcgatc gcattcaggc ggcgcttgat gagtggcatc gcagtaatcc
    23281 ggggccggta aaagataaag cggcctataa atctttcctg cgtgaactgg gctacctggt
    23341 gccgcaaccg gagcgcgtga cggtggaaac cacgggcatt gacagcgaaa tcaccagcca
    23401 ggcggggcca cagctggtgg ttccggcaat gaacgcccgc tacgcgctga acgcggcgaa
    23461 cgctcgctgg ggctcactgt acgatgcgtt atacggcagc gacatcatcc cgcaggaagg
    23521 ggcgatggtc agcggctacg atccgcagcg cggtgcgcag gttatcgcct gggttcggcg
    23581 tttcctcgat gaatcactac cgctggaaaa aggcagctat caggatgtgg tggcgtttaa
    23641 ggtggttgac aaacaattac gcatccagtt gaaaaatgtt aaagaaacca cgttacgtac
    23701 cccggcacag tttgtcggtt atcgtggcga taccgctgcg ccgacctgca ttttgctgaa
    23761 aaataacggc ctgcatattg agctgcaaat cgatgccaac gggcggattg gcaaagacga
    23821 tccggcgcac atcaacgatg ttatcgtcga agctgccatc agtaccattc tcgactgcga
    23881 agattcggtc gcggcggttg atgcggaaga taaaatcctg ctgtaccgca atctgctggg
    23941 cctgatgcag gggactctgc aagagaaaat ggaaaaaaac ggtcggcaaa tcgtgcgtaa
    24001 actgaatgac gatcgtcatt acaccgccgc cgatggctct gaaatttctc tgcacggacg
    24061 ctcgttgctg tttatccgca acgtggggca tttgatgacc attcctgtga tttgggacag
    24121 cgaaggcaat gaaatcccgg aaggcattct tgatggcgtc atgacaggcg cgattgccct
    24181 ctatgattta aaagtgcaga aaaactcgcg cactggcagc gtctatattg tgaaaccgaa
    24241 aatgcacggt ccgcaggaag tggcgtttgc caacaaactg tttacccgcg ttgagacaat
    24301 gctcggtatg gcaccgaata ccctgaaaat gggcattatg gatgaagaac gccggacctc
    24361 gctgaacttg cgtagctgta tcgctcaggc gcgtaaccgc gtggcgttca tcaataccgg
    24421 tttcctcgac cgtaccggcg atgaaatgca ttcggtgatg gaagctggcc cgatgctgcg
    24481 taaaaaccag atgaaatcga cgccttggat caaagcctac gaacgtaata acgtgctttc
    24541 cggcctgttc tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt gggcaatgcc
    24601 ggacctgatg gcagacatgt acagccagaa gggcgaccaa ctgcgtgccg gggcaaacac
    24661 agcctgggtt ccgtcgccaa ctgccgctac gctccatgcg atgcactacc accaaaccaa
    24721 cgtacagagc gtgcaagcca acattgccca gaccgagttc aatgctgaat ttgaaccgct
    24781 gctggacgat ctgctgacta ttccggttgc tgaaaacgct aactggtcgg cgcaagagat
    24841 ccaacaagag ctggataaca acgtgcaggg gattctgggg tacgtggtgc gctgggtgga
    24901 gcaggggatt ggttgttcaa aagtgccgga tattcacaat gtggcgctga tggaagaccg
    24961 cgcaacgctg cgtatctcca gccagcatat cgccaactgg ttacgtcacg gtattctgac
    25021 caaagaacag gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc agcaaaacgc
    25081 tgacgatccg gcttatcgtc cgatggcggg gaatttcgct aactcgtgtg cttttaaagc
    25141 tgccagcgat ttaatcttcc tcggcgtgaa acagccaaac ggctataccg aaccgttatt
    25201 acacgcctgg cgtttacgcg aaaaagaaag tcattaatag agtcaaaaaa agccccggca
    25261 agttttattg tcggggctat tatagtaact gcggtagtac atttatcccg gaaataaccg
    25321 gagcgattat cagggtttaa caatattaaa tacccgtagc gcattactag cgggcctata
    25381 acaacaatat ggacatctta acgatactct gcgcctgcgt gtgcagaaca gaaaccctta
    25441 cccgatatac ttgttatgcc agaggtagca attgcgttag cggcgtactg ttgctgtaaa
    25501 gtataacgcc tatattttac agttcagtcc tcttttgctt attacaacct taagtatatc
    25561 aagcatataa agataataag agactgaaca atatggttac ctggacccaa atgtatatgc
    25621 cgatgggagg actggggcta tccgctctgg tcgccctgat cccgataata ttcttcttcg
    25681 ttgcactcgc ggtattacgt ctgaaaggac atgtcgctgg agcaataacc cttatattat
    25741 ctatcctgat tgcaatattc gcctttaaaa tgccgattga tatggcattt gctgctgcgg
    25801 gctatggctt tatttatgga ttatggccaa ttgcgtggat tattgtcgcg gcggtgttcc
    25861 tgtataaatt aaccgttgcc agcgggcagt tcgatattat ccgcagctcg gttatctcca
    25921 tcaccgacga tcagcgtttg caggtgttac tgattggttt ctcctttggt gcgttgctgg
    25981 aaggagcggc tggctttggt gcgccggtgg cgattaccgg tgcgctgctg gtgggcctgg
    26041 gcttcaaacc gttatacgcg gcggggctgt gtctgattgc caatactgcg ccggtggcgt
    26101 ttggtgcgtt gggcgtgccg attctggtcg ccggtcaggt aacgggaatc gatccgttcc
    26161 acattggcgc aatggcggga cgtcagttac cgttcctgtc ggttcttgtg ccgttctggc
    26221 tggtagcaat gatggacggc tggaaagggg tgaaagagac gtggccagcg gcgctggttg
    26281 ctgggggaag cttcgctgtc actcagttct ttacctctaa ctatattggt ccggaactgc
    26341 cggatattac ttcggcgctg gtgagtatcg tctcactcgc tttattcctt aaagtctggc
    26401 ggccgaaaaa taccgaaacg gcaatcagca tggggcaatc cgcaggtgcg atggtggtaa
    26461 ataagccatc ttctggcggt cccgtgcctt cagaatatag tctggggcaa atcattcgtg
    26521 cgtggtcacc gtttttaatc ttaacggtgc tggtcaccat ctggaccatg aagccgttta
    26581 aagcgttatt tgctccgggc ggcgcgtttt attcactggt gattaatttc cagatccctc
    26641 atttgcatca acaagtgttg aaagcggcac ccattgtcgc ccaaccaacg ccaatggatg
    26701 cggtgtttaa attcgaccct ctctcggctg gcggcaccgc tatttttatt gcggcgatta
    26761 tctctatctt catcctcggc gtgggcatca agaaagggat tggcgtcttt gccgaaacgc
    26821 taattagtct gaagtggccg atattgtcga ttggcatggt gctggcgttc gccttcgtca
    26881 ccaactattc cggcatgtcc accacgctgg cgctggtact ggcaggtaca ggcgtgatgt
    26941 tcccgttctt ttcgccattc ctcggctggc tgggggtatt ccttaccggc tcggacactt
    27001 cctctaacgc cctgtttggt tcactgcaat cgaccacggc gcaacaaatc aacgtctctg
    27061 acactctgct ggtggcagca aataccagcg gcggcgtaac tggcaagatg atctccccgc
    27121 aatctatcgc cgtggcctgc gccgcgacag gcatggtggg ccgagaatct gaactgttcc
    27181 gctacaccgt gaagcacagt ctgatttttg ccagcgttat cggcattatc actctgctgc
    27241 aggcgtatgt gtttaccggg atgttggtaa gttaatagaa tagttatgcc tgacgttaat
    27301 acaaacccag tctatcagac tgggtttgtt agtatttccg atatcagaca gggaaaatgc
    27361 ctggtcagaa atgaggcgta attcggtttt ctaaaaaaag taaaaaaaac gccttgtggg
    27421 tggggattat cttacgacat gcactttgtt ccatactttt aaactcccca ttgttatgaa
    27481 gtaaaggcgt tgtcttttta tccgttcaaa ctttagctca aatttgtagc atgcagattc
    27541 cggtattttt cgtgatacca gaaaaaagtc actggaggaa atgttgtgat aaaaatcaca
    27601 aagtaagtca ggttactgga gataaagtct gcaacagaga attatcgtag tctgtgtacc
    27661 tcaaccagta ttttaaaggc ccagcttcat ggaagaaaac acaaggcagc gtacagaaaa
    27721 ttatatttca gcaaaaaacc agcatcccgc ctggatactc cttgccactc gccgtgctcc
    27781 actggtattg agttgcctga aaacgctttt tgaaaaatca catgatggca ttccccttga
    27841 ggaagctatt cagtctcttt ccagtattct cattgagcat gtgagtcagg aacagtatga
    27901 cattaatcag gacaatccat ttcttcaggc cagtcgtgaa ctgcgggagt ggattaaacg
    27961 ccgtttaatt gtcgaacggg atggccgtat ttttgctacc gatgcgcttg aggtcgcgat
    28021 tacgtttgta gagtctctgg ataatcgttt tatgacctct acggcttccc gtttgtccac
    28081 cgttcagcga gaaattgaaa acctggaaac acggcttaat cctaacccgg caaaccgggt
    28141 tgcaacctta cgtcgtcgta tttctgaact ggaacgcgaa ctacaggagg cagaagccgg
    28201 gcatattgag gtgctggaaa cgcaccaggc ggttgagcat attcgtgagg tctacaattt
    28261 agcttccagc ctgcgggctg actttcgtcg tgttgaagac tcctagcgtg aggctgaccg
    28321 cgctcttcgc caatccatta ttggtgagca atatcatcgc ggcgatatag tagaacgcct
    28381 gctcaacgat caggacgcgc tgcttaatac gccggaagga agagttttcg acagctttca
    28441 gcaacagctc cgtcaatctt ctgaacttaa agcgatgagt gaacgattac gtgtgatcct
    28501 tagccatcct tctgcttctg atgcgcttaa ccgtctacag cgtcacgatc tgcgctggct
    28561 ggttaaacgt ctggtcgatg aatcgcaaac tgtcctccag gctcgcgccc gtagcgagcg
    28621 tgatgtccgt ggtttcatga aaaccggtct tgctgctgaa catcatcgtg ttggtcattt
    28681 gcttaatgag tttctgaatc tggcgttaaa actcgactgg cagcgccaga tgattcgtaa
    28741 acaagaggtg ccgctacctg ccgttggtgt ggcggtaaca ggcatccccg ctattgaacg
    28801 tttacgtttt aaagaggtgg atgatgaagc agagcagacg ttggatctca gtaatcatgc
    28861 ggcggattta acgcagattg gcgatgattt ctgggatgcc ttcaatggcc tcgatcgtga
    28921 ggtgttgatt cagcagacgt tgcagcttct tgcaaaagaa aatcgccccg ttggcctggc
    28981 tgaactggcg gcgctgttac ccccggctca cgatctcgaa acattcgccg tctgggttgg
    29041 tatggcaaga gaagcgggta ttgaggttat tgattcacag cgtgaatttg cagagcttag
    29101 tgatggggaa ggtcgccgct ggcgatttaa tctgcccacc accggtctgg aaagtcaggc
    29161 attaatggat atcgattggg agtaacaggt atgacgggct tttttgacag gctgattaac
    29221 cggagcgtta ccgcaaatgc aggctgtgaa ccagaaccat cggatgagga ggttaccgat
    29281 gagtcggttg aagaaagttt ggccagtagt gaaacgcgga cgctaaaaaa aatacgtgaa
    29341 gcgactcagg aattgctcaa atacggcctg ctggaagagg ccagtaagcc caatctgtac
    29401 cgcatcgtac tgagccatcc tgaagaggtt actcgtattc tggagcctct cgatctggat
    29461 atcggcattg atgagatccg tggcctgctg tatgtcaaag tcaggcttga tgaaacgcct
    29521 gcgcaggatg aatgggccca ccttctggtg aggcggcaac gactaaacct ggaacaatct
    29581 ttattagttg ccatactgcg ccaacatttt gtggcctggg agcaggaaag cggaaccggt
    29641 gccagtcagg cacagatcgc catcgatgat cttctccctc aactccagat ttatcttggc
    29701 gacccgggca gtgaatcaaa agaacgcacc cgattgctta ccctgctgga tcaattaaag
    29761 gggcatgggc tggtgacgtc gccggatgca catgagcgga tcgtcatccg tccgatcatt
    29821 gctcatcttg ccgatccgat caatcttcag gcattactgg catggctgcg agagcaaatt
    29881 gcccagcaaa cctctccaaa tgacgcgcga gaaaaggata gcagcgagga ggacgtatga
    29941 atcaggtatc tggcctggct ggaaaggaat ctttcattct tacccgtatt gagttgttca
    30001 actggggcgg ttttcatgga ttgcatcagg cagcaatcca tcaggatggt accgccgtta
    30061 ttggcccaac cgggagcgga aaaacaacgt tggtcgatgc cctgatgact ctgctttgtg
    30121 ccaatcctcg ctacaatctg gcctcaactg gcgggcatga aagcgacagg gatcttatct
    30181 cttacgtccg cggcgtatcc ggtcctggcg atggcggtga agggcaatcg catattgcca
    30241 gaccaggtaa aaccgtgaca ggtattgctg ctacgctgga gcgcgaagga aaacaggtcc
    30301 gtctgggggc gttattgtgg tttgattcca ccagttccag cgtgacggat atgaaaaggc
    30361 tctggctgtt cagcgataat cccgggcaga cgctggagca ttggctaaac gtttaccatg
    30421 agggaggtac acgtttgctc cgacagatgg aaaaagaagc catcgggctc tggacatacc
    30481 cgaataaaaa gcagtatctg gcgcgcctgc gggatttctt cgaggtgggg gaaaacgcgt
    30541 ttacgttgtt aaaccgggcg gcagggttaa aacaactcaa cagtattgat gagatttttc
    30601 gcgagctggt gttagacgat cactccgcct ttgatcgcgc ggctgaagtg gccaatagtt
    30661 ttgatggtct gactgagatt catcaggaac tggaaaccgc tcgcaaacaa cagcagtctt
    30721 tacaacctgt cgccctgagc tgggagaagt accaaaagca agagcgtcaa ctggctgact
    30781 ggcagacgct ggaatcgctg ttacccctct ggtttgcaca acaggcaaac cacctctggc
    30841 gggaaaagat taaccttctt aacgcaaggc tcgctgaggc acaaaccagc gaagaacagc
    30901 tccagtcaca gcttgatttg caaaaaaagg tggttagcga ctgtatgcag cgttatctcc
    30961 aggtcggcgg tgccaatatt gatgaactga acgagcgtat caaggactgg caaaaaacgc
    31021 tcggcagtcg agaggcattg gcgcgtcaat atcaacagct gacccgtaac cttggattgc
    31081 cgtctgatct gatccaacca cagctggagg cgaatcagca tgaagccgcg gccctatgtg
    31141 agcagatcgc cgttgatata aagttgaaac aggaagaagc gtatcaaaaa ggtgcgctta
    31201 gccatcacat taccgaagag cttcgggaac gggaaaatga acgcgccgag atcgcgcgtc
    31261 gtccggattc taaccttccc gcacattatc aggcgtttcg tagcgagctg gcaaaagcgt
    31321 tgaacgtgga tgagtctgaa ctgcctttcg tcgctgaact gatacaggtt aaaccggaag
    31381 aggcgcaatg gcgcggtgcg attgagcgtg ctgtcgggag taacaggctg cgtattctgg
    31441 ttgcgcctga atcggcacag gaagcgctgc gctgggtaaa tcagcgcaac aaccgtctgc
    31501 atgtgcggct actggaggtc aaattaccgc actctcccgc ccggttcttc gacgatggct
    31561 ttacccgcaa gctgttgtgg aaagatcatc cgtggcgaga ggccgtcaaa gcattactgg
    31621 ccgaaagcga caggcattgt gttgacagcc ctgaacaact gcgtgacacg cctcatgcta
    31681 tgacggtgca gggattaatg tctggtaaac agcgttttta tgacaagcat gatcagaaac
    31741 gccttgatga agactggctg acgggattcg ataaccgcga tcgcctgaac ttccttgcta
    31801 aagaaattac cacgcttcag gagcaggtta aaaccgcaaa cgcggcattt gagtttgcga
    31861 aaggcgaggt ggggcttttg caaaatcagg ctgcttcctt ccagaaaata gaacaaattg
    31921 atttcgatag catcgatgtt cccggcgcga agagccaact ggacgcgctc agggagcggc
    31981 tggaaaatct gacccgcccg gattctgacg ccagtgtagc gaaagcaaaa cttgatgagg
    32041 ctcaaacggt tgaatctgaa ctcgataagc agctcagagc tgccaataaa gtcactaacg
    32101 tgctggatac agaactcaca tcagcccgtg ccgcggaacg taaggcccag caaactgcac
    32161 aacaaggtat gaaggaagaa gagcgagagt tgtgcgcgtc acattttcca gtggtgactt
    32221 tggagcaatt gcctgacatt cgcgatttag aacgtcaaca tgagcgggga attcagcatg
    32281 aaattgaaag agttaaagct gaactacatc gactgaacat tgagttgacc aaacgtatgt
    32341 ctgaagccaa acgtgtggat accggggcgc tcgttgaggc aggggctgat ttggacgata
    32401 ttactgttta tttacagcgt ttgcaggaac tgaccgaaga agccctgccg gaaaagctca
    32461 atcgcttcct tgattatctt aaccgttcat ccgatgacgg cgtaacacaa ctgcttagcc
    32521 atattgagca tgaggtgctg gtgattgagg agcgcctcaa tgaactcaat gaaactatgt
    32581 tcagggttga ttttcagccg tatcgctacc tgcgtctgga taccaaaaaa gttgtccatg
    32641 aaagcctgcg cacgcttgaa aaagcacagc gtcagctaaa tgccgcccga tttgttgatg
    32701 ataatggcga gagccattat aaggctttgc agatactggt ggcgcaattg cgggatgctt
    32761 gtgaacgaaa ccgtaccctg ggagcaaaag cgctactgga tccccgtttt cgactggagt
    32821 tcgccgtatc ggtgatggat cgtcagagcg gaaatgtgat tgagtctcgt accggctcac
    32881 agggtggtag cggtggagaa aaagagatta tcgcttctta cgtgttaaca gcttcactta
    32941 gctacgcact ttgtcctgcg gggagccgtt atccgctttt cggtaccatt atccttgatg
    33001 aagccttctc ccgcagttct catgcggtgg cgggtaggat cattgccgcg cttagagaat
    33061 ttggcttaca tgcagtattt atcaccccca ataaggaaat gcgcctgctg cgtgaccata
    33121 ctcgttcggc gattgttgtc caccggcgcg gtcagaattc caatatggct tctctgagct
    33181 gggaagagct tgaacgacac taccagcgac gggggaacgc gtaggatgta ttccccagat
    33241 gaattgcgtg aaaagcttgc ccggcagtgg gatagcgcaa aacttcgggc tgaacgctta
    33301 ctgtcccccg gtaactggcc gctctgcctg cctattggta aaccttccac gaaaatattt
    33361 gcagagcaga ctcagcgcgt tttgcagcac gtacagcgat ggaggcaagt tgcggttggg
    33421 cacgtggaat gggaagcggt gagttatcgg gcaagtgata caccagtatt aatgccgttg
    33481 cgctggattc tgaatagccc ttctgagtgg atcaacgccg ctgcggatcc ggttgtgagc
    33541 cgggaatttc gcttgctgga aggaattatt gaacaagtta atcccatttt tcacccgctg
    33601 ttggtaaagc atcgctctct ctggcggcac aaagatccgc agggcgtcat cagcgccgcc
    33661 acgctggcct gccgattaga gccgggctgc gcaaaagggc tgccgttaag attgctttcc
    33721 gggcaaggag tggataccaa atttatcgag aataatattt cgttattaac ccgactgtta
    33781 gacgtgcggt tttcaggaga agccagcgag cagggattaa caacttttct tgatgctttt
    33841 gatgagtcca gtcactgggt tttagttgta ccattatcac ccggcttact tccttttaaa
    33901 aaatgccggg taacaacggc ggaactggca gaaacaacat tacctgcgtc gcaagtactg
    33961 gtggttgaaa atgagcagtg tctgcatcat cttccagcat tatctgacac tatcgcgatt
    34021 ttaggctgtg gcctggacgt gcaatggcta tccagttctg ttctggatga aaaacgcgtt
    34081 gcttattggg gggacatgga tagctgggga ttactgatgc tggcgagagc caggcgctgt
    34141 cgtccgacgc ttgatgccct gctcatgaac cgtgagttgt ttgagcagta tgccagtcat
    34201 agcgccgttc cagaaccggt catagcgaag gaagctgtac ctgatggctt gcttaacgaa
    34261 gaggccgatt tttatcgcta cttaacccgt ttaccgtgtg gtcgtctgga gcaggagttt
    34321 ttgccagtgg ggattgcgga agaggtgctt ctcaggtggg gaaaagagag ttaaaaaagt
    34381 catgcttgct ctggaaggtt atttacgcgg tattttgtag tggttgttgc gaaagtctgc
    34441 gttgatggtc ctgatacctt accgggtaga gggactttcc atcagtcagt ttagatactg
    34501 tggcaactgt cgcctttagg tggacaaatg cataaattat taaactatta aatttcttgt
    34561 tttcaggcag cagagagaag gtatatcaga gggtactgag gtgtataaac gtcacgaaca
    34621 tccgtgaaaa ggcgaaaaaa accagccaat agataatgct ttgcaatggc cagaaatcta
    34681 ccgtagccag aaagtgaaaa agccccgctt tgcagcgagg ccttatgaat agtggtgccc
    34741 ggactcggaa tcgaaccaag gacacgggga ttttcaatcc cctgctctac cgactgagct
    34801 atccgggcaa cggggcgcat taaacctgat tcgcctcgtc tcgtcaatca aatttcttca
    34861 atttactgca gactgcacaa tctatcatca ctttgccgct attgcacgca ttgtcaggca
    34921 aaccaggcgg tccaggcggc ggaaagcggc attgccagaa ggagtgctgg aagcatgttg
    34981 accaccggga acattttaat gccacagatg cgtaatccgg ttgccagcag caataaaccg
    35041 cctacggcgc tgaagtctgc catcatcgac ggtgtggtca gcggtaatat cagcgcggca
    35101 gcccacgcca gcgttaactg gatgatcagt aatgggatac taattaccga caccgcaata
    35161 cccagcgagc aggcgaagat catcgccgta aagaaatcaa gaaatgactt ggcgattaaa
    35221 atactcggat cgccggtcat cccttcgttc atcgccccga agatcccggt gccgctggcg
    35281 caaaacagga caataatcgc gacataattc tgaataaaag attcatgcgt tggcttctta
    35341 cgtgagtgac gaaacagatt ttgtgctttg gcgactgctg tattgacacc tttctccagc
    35401 aaacaaattt cgccaattag agcgccaagt agcgtagcta aaaccatcgc tggaaggttg
    35461 gcgcatttca caaccagtaa aataccaatc cccagcgatg ccagaccaaa aatagatgtc
    35521 atggagacgc ggatacgttc cggtaagcgt tggctgagca gtgcgccgag aacgccaccc
    35581 agtaagactg cactagcgtt gataaaaggg ccgatgacca caagagttcc tgttagctgt
    35641 attgtttgat tttcttatta tgaacttttt gccttgttga tggcttgtct ggtcgcaaag
    35701 tcctggcttg cacgctttag cgaaaggtgc catgattgcg cgaattttct cctctctgta
    35761 cggagtttgc ccgatgcacg ccacctcctt acattctctc gcttatcgcc gtttcgcgcg
    35821 aaacgtttcc ctttctatgt tactgctcat gcggtgaagt taacgcacgc tcactgcagg
    35881 acaacagtaa aatcagagcg tttctgcttt tactgatgtc tggcggtcgg agctggtgac
    35941 cagtttgacc catatctcat ggggcagggt tttccacctt gtccggtatt cttacttccc
    36001 cgaaacgggt ttgcgcttat gaaatcaatg aatattgccg ccagtagtga actggtatcc
    36061 cgactttctt ctcatcgtcg cgtggtggcg ttgggagata ctgattttac ggacgtcgcg
    36121 gcagtcgtca ttaccgctgc ggatagtcgc agtggcattc ttgcgttgct taagcgcacc
    36181 ggttttcatc taccggtgtt tttgtattcc gaacatgctg ttgaattacc tgcgggcgtt
    36241 acggcggtaa tcaacggcaa cgagcagcag tggctggagc tggaatccgc agcctgtcag
    36301 tatgaagaga atttgctgcc accgttttat gacacgctga cgcagtacgt tgagatgggc
    36361 aacagcacct ttgcttgccc tggacatcaa catggtgcgt tttttaaaaa gcatcctgcc
    36421 ggacgccatt tttacgattt ctttggtgag aacgtctttc gcgccgatat gtgtaacgct
    36481 gacgtaaaat tgggcgatct gcttattcat gaaggatcgg cgaaagatgc gcagaaattc
    36541 gcagccaaag tctttcatgc cgataaaacc tattttgtgc tgaacggcac atcggcagcg
    36601 aataaagtgg tgacgaatgc gctgttaacg cgtggcgatc tggtgctctt cgaccgtaac
    36661 aaccataagt cgaatcatca cggcgcgctg attcaggcgg gggcgacgcc ggtctatctg
    36721 gaagcttcac gcaacccgtt tggtttcatt ggcggtattg atgcgcactg ttttaatgaa
    36781 gagtatctgc gccagcaaat tcgcgacgtt gcgccagaaa aagctgagtt gccgcgcccg
    36841 tttcgcctgg cgattattca gctgggaacc tatgacggca ctgtctataa cgcccgtcag
    36901 gtgatcgata ccgttgggca tctgtgtgat tacattctgt ttgattccgc gtgggtcggt
    36961 tatgaacaat ttatcccgat gatggaggat agctcgccgc tgctgttaga acttaacgaa
    37021 aacgatccgg ggatctttgt gactcagtcg gtgcacaaac agcaggcggg attctcacag
    37081 acgtcgcaga tccataaaaa agataaccat atccgcggac aggcgcgttt ttgcccgcat
    37141 aagcggttga ataatgcctt tatgctccat gcttctacca gcccgttcta cccgctgttt
    37201 gccgcgctgg acgttaacgc taaaattcat gaaggagaga gtgggcgtcg gctgtgggct
    37261 gagtgcgttg agttgggtat tgagtcgcgc aaggctattc ttgcgcgctg taagctgttc
    37321 cgcccgttta tcccgcccgt tgttgatggc aaattgtggc aggattatcc gacatctgtg
    37381 ttagccagcg accgccgttt tttcagtttt gagccggggg cgaagtggca cggctttgaa
    37441 ggatatgccg cggatcagta ttttgttgat ccgtgcaagc tgttactcac tacaccaggt
    37501 atcaatgccg aaactggcga atatagcgac tttggcgttc cggcgacgat tctggcgcac
    37561 tatctgcgtg agaacggcat tgtgccggag aagtgcgatc tcaactccat tctgttctta
    37621 ttaactccgg cggaaagcca cgagaagctg gcacaactgg tggcgatgct ggcgcaattt
    37681 gaacagcata ttgaggatga ctcgccgctg gctgaggtgt tgccgagcgt ttataacaaa
    37741 tatccggtgc gctatcgcga ctacactttg cgccagttgt gtcaggagat gcacgatctg
    37801 tatgtcagtt tcgacgtcaa agacctacaa aaagcgatgt tccgccagca gagtttcccg
    37861 tcagtggtga tgaaccccca ggatgcgcat agcgcttata ttcgcggtga agtggagttg
    37921 gtgcggattc gtgatgccga agggcgaatt gcggcagaag gggcgttgcc ttatccacct
    37981 ggcgtgcttt gcgtggtacc cggggaagtc tggggtgggg cggttcaacg ttatttcctt
    38041 gcactggaag aaggggtgaa tttgttgccg ggattttcgc cggagctgca aggtgtttat
    38101 agcgaaaccg atgcggatgg catgaaacgg ttgtacggtt atgtgttgaa gtaagaataa
    38161 aaaaaacggg tcgccttctg gcgacccgat tttctttgcg taattagtgg ctaaccgtct
    38221 gtgtacctgt cgggacacga acgtgtttat atttgaacat cgccatgaac gcgaaggcca
    38281 gaaccacgga gtaaccagcg aaaatcaacc atacggtctg ccagtcggta atgccgtttt
    38341 gggtgtacat ctcaacaact ttaccgctca cgatgccgcc gaggatacag ccgaagccgt
    38401 tagtcatcat caggaacatc ccttgtgcac tggcgcgaat tgccgggcta acttcttttt
    38461 cgacaaacac cgaaccagag atgttgaaga agtcgaatgc gcaaccgtaa acgatcatcg
    38521 acagtaccag cagtacagta ccgaacggag tcgggtcgcc gtaagcaaac agcgcaaaac
    38581 gcaggatcca cgccacaata ctgatcatca ttacgttctt aataccgtag cggcttaaga
    38641 agaacgggat ggtcagaatg aacagggttt cagagatctg cgaaatcgac atgatgattg
    38701 acgcatgctg cacaataaag ctgctggcaa acatcggatc tttgtcgaag ctgtgcagga
    38761 aggtattacc gaacatgttg gtaatctgca gttccgcgcc cagcagcatt gagaagatga
    38821 agaagattgc catacgcttg tttttaaaca gcgcgaatgc atcgaggccc agcagggttg
    38881 tccagctctg attcgcttgc tgtttagcaa ccggaatatg cggcagagtc agggtaaaca
    38941 gaaccagaac ggcggaaaga gctgcgccaa tatacagctg catgtggctt aattcgaagc
    39001 cagacaggct caccacccac attgccatga taaagccgat ggtgccccag atacggattg
    39061 gcgggaagtc agtaacgata tccatcccgg cattttgcag gcgatagtaa gagatggtgt
    39121 tgattaaccc aagcgttggc atataagcaa acgagttaat caatatcaca aggaacatcg
    39181 cctccggcgt cgtgacctgt gccgccatga acagcgtgat agcgccaatg gtgtggcaaa
    39241 tggcatatac ccatttcgca cttaaccatt tgtcggccac aatccccagc agcgcaggca
    39301 taaagaccgc ggcgataccc agtgagctat aaactgcgcc aatagaagca ccgtcaaact
    39361 tcagggtaac aaacatatag gagccgaggg tcgtcagcca acttccccac agacagaact
    39421 gcagaaaaga gaggattttc agctgcagct taagattcat gttaatttcc tcacatcgtg
    39481 atgcggatga gtgtttgaaa aagcacattt gaccgtgcga gatgatggga ttctatcaac
    39541 ttcgcggact ggttttttgt tacctgtggc aaataattgc aaacattccc aatttgcaaa
    39601 acgaaattgt gatatggata acatttttgc ccctgagcat cgtcaggggc ggttaatgga
    39661 attatcggcg gcggtaggat ttttgcgcgg tattcacttt ataaagataa cggcgagatt
    39721 ccgcagaggg atggcgggtc gtcagcgtct gataaacatc gcccggcgtc atggtgttaa
    39781 taatattggc cgcctgaatc ttatcattcg aaaagactcg cagcacgctg cctgcgccgc
    39841 cgttataggc ggtgatgacg gcataacgcc gtgacgttgg gttatcaatt ccgccgagat
    39901 aaacattgtt cagcatcgcc agatacgcgg tgccggtatc aatattgctg gcaggatcaa
    39961 acaagaaact gcggctcggc gtgccggatt tcccctgcga gcggaacaca tctttcccgg
    40021 cagtatgttg caccacctgc attaacccca gcgcatcgga acggctgacc gcatacgggt
    40081 taaaggaaga ttcagtctgc ataattgcca gaatcagcga ctcatcaacg ccatatttcc
    40141 gtgacgcctg gcggaccatg ccgagatatt tgtgcgcacg tttatcaagg tggttcggca
    40201 ccatgttaat ggtgacgctg tagatgatac gcagcccgtt gctgcggctt ttcagacggt
    40261 ttttcagcag ataatccgcg aagttgcttg cgcgaccttc ccagcgaatc ggctgcccgg
    40321 tgttgtccac cacctgaccg taaaggaaag gttctttcga aattgtaata tcatcaacgt
    40381 cggaatagag atcgaccgaa ctcggatcgt cacccatcag caacgttttg ataattgccc
    40441 ggcgcaaatg cgcggcaggt tctgtcccgg cgatggtttc gatagtaatc gtaccgtcat
    40501 cgaagttgat gtggctgcgg gtctgatatt gatcggtgta tttcacgtag tccttaggac
    40561 cagcgatcac cacctctttg aagccccaga tgttctcaat attgtgggca aattgcccca
    40621 tcagaatatc aaaaccgttg gtgtctttga cccaggcttc gttataggta tcgccttttt
    40681 tggtcgtcga acaggagatg agcaacggcg caatcaaagc cagcgcgaga tattttttca
    40741 tcattccggg tgcgtgttgt gtttgttttt atgcaagctg ccggaggcgt gctccggcac
    40801 tgtttttatt ttttatcttc cggcgtatag ccctcgatat gcacctcttt accctcgaac
    40861 aggaagttga ccatctcctg ctccagcagc ttgcggtgct cggcattcat catgttgagt
    40921 ttcttttcat taatcagcat ggtttgtttg tgctgccact gcgcccaggc ttctttggag
    40981 atctcgttat agatgcgttt tcccagttcg ccggggtaca gctgaaaatc ctgaccttct
    41041 gcttcacgtt gcaggaaagt acaaaaaatc gttctgctca taaatcatcc tctttatcga
    41101 ctcacgcgct aaaccggcgc gccagtgcgt aactgctgta acaaacgctc cacgggagcc
    41161 gccaggccaa ctgacggcgg ttgcgctaag ttataccaga gcgcattgcc ttcatccatg
    41221 cagccggtga atgacgacac gggaagccac ataggcacaa tatctaagtg gaaatggctg
    41281 aaggtatggc gaaacgcggt cagctgcgtc aggttatcgg cagcaatctg ccgttgcgcc
    41341 agccactgcc gcaaactttc ttcgtcggca aactgcggga aacagtataa accgccccac
    41401 aatccgctcg gcggacgctg cgccagtaat acttcatctt cgtgctgtaa cagcaaaaag
    41461 tagccggtgc gctccggcag agtctgtttc ggttttttgc ccggataaag cgaccagcta
    41521 ttgttggtgg cggcaataca tccgttttgt agcggacaga gcgaacattt cggcttcgaa
    41581 cgcgtgcaaa tcatcgcgcc caaatccatc atcgcctgat taaaccgttc cacgccgacc
    41641 gcgggcgtca cctgctcgct taaactccat aatttattct cgacctcttt tttcccaggc
    41701 cagccgctta cagcatagca gcgcgccagc acccgtttga cgttaccgtc gagaatcgga
    41761 aagtgcttac ccagagaaag cgagagaatc gcgcctgcgg tggaacgccc gacgcccggc
    41821 agtgctgcaa cttcctcaaa ggtttccggg aatttaccgc cgtgtaaggt cgccacttgt
    41881 tgtgccgctt tatgcagatt gcgcgcgcgg gcgtaatagc caagcccggt ccacaagtgg
    41941 agaacttcgt cgagcggcgc attggcgaga tcggtcaccg tcgggaagcg cgccataaag
    42001 cgttcaaaat aggggataac ggtcgcaacc tgagtttgtt gcaacatcac ttctgagagc
    42061 catactttgt agggcgtctt gtcaatttgc cagggcagag tttttcgccc gtatttatcg
    42121 taccagtcca gaacctgggc tgaaaattgc gacgcttgca tggtcaccga attcactgtt
    42181 gttgggggca agattgcagc acagcgacgg cagggtgtaa accggaactt tccgcagcca
    42241 cggccttatc atgcttgcat ccggcaacta actttggata atgcccgttt tcagaacact
    42301 ttcacaagcg actaaatctt tatgaaaaac gacgtcattt caccggaatt tgatgaaaac
    42361 ggccgcccac tgcgccgtat ccgtagtttt gtgcgccgcc aggggcgact gaccaaaggc
    42421 caggaacatg cgctggaaaa ctactggccg gtgatgggcg ttgagttcag cgaagatatg
    42481 ctggatttcc ccgcgctttt tggccgtgaa gcgccggtga cgcttgagat tggttttggc
    42541 atgggggcgt cgctggtggc aatggctaaa gatcgccccg agcaggactt cctcggcatt
    42601 gaagtgcatt caccgggcgt tggtgcgtgc ctggcttctg cgcatgaaga gggcttaagc
    42661 aacctgcgcg tgatgtgtca cgatgcggtt gaagtgctgc ataaaatgat tcctgacaat
    42721 tcattgcgca tggtgcagct ctttttccct gacccgtggc acaaagcgcg ccataataaa
    42781 cgccgtatcg ttcaggtgcc gtttgccgaa ctggtaaaaa gcaaactgca gctggggggc
    42841 gtattccata tggcgaccga ctgggaacct tatgcggaac atatgcttga agtgatgtct
    42901 tctattgacg gttataaaaa cctgtcagag agcaatgatt acgtaccgcg tccggcatca
    42961 cgtccggtga cgaaatttga acaacgtggt catcgtcttg gtcacggagt atgggactta
    43021 atgttcgaga gggtgaaata atggcaaaga accgtagccg tcgtctgcgt aaaaaaatgc
    43081 acatcgacga attccaggaa ttaggatttt cggtggcatg gcgattcccg gaaggtacat
    43141 cggaagaaca gattgataaa actgttgatg attttattaa cgaggttatc gaaccgaaca
    43201 aactggcctt tgacggcagc ggttatctgg cctgggaagg tctgatctgc atgcaggaaa
    43261 tcggcaaatg caccgaagaa catcaggcga ttgtgcgtaa gtggctggaa gagcgcaaac
    43321 tggatgaggt acgcaccagc gaacttttcg acgtttggtg ggactaagaa agcatacggg
    43381 cgatgacaaa tgcaaaactg cctgatgcgc tacgcttatc atgcctacaa agatgcacga
    43441 tcgagtaggc gggataaggc gtttacgccg catccggcat ggaaaacgcg tactttgtta
    43501 tcaatctggg gccagcaaat gctggcctga tttgttcttg agggaagact atgatgcgca
    43561 aaatgctgct ggcggcagca ctttcagtga cggcaatgac cgctcacgcc gactaccagt
    43621 gcagcgtcac gccgcgtgac gatgtgattg tcagcccgca aaccgtgcag gtgaagggcg
    43681 aaaacggcaa tctggtgatc acgccagacg gcaacgtgat gtataacggt aagcaatatt
    43741 ccctgaatgc cgcccagcgc gagcaggcga aggattatca ggctgaacta cgtagcacgc
    43801 tgccgtggat tgatgaaggc gcgaaaagcc gcgtcgaaaa agcccgtatt gctctggata
    43861 aaattatcgt tcaggagatg ggcgaaagca gcaaaatgcg cagccgtctg accaaacttg
    43921 atgcgcagct gaaagagcag atgaaccgca ttatcgaaac gcgcagcgat ggcctgacgt
    43981 tccactataa agccattgat caggttcgcg ccgaaggcca gcaattagtg aatctggcaa
    44041 tggggggaat tttacaggac agcattaatg aaatgggcgc gaaagcggtg ctgaaaagcg
    44101 gcggtaaccc attacagaat gtgctgggaa gcctgggggg gctgcaatcc tcaatccaaa
    44161 ccgagtggaa aaagcaggaa aaagatttcc agcagtttgg caaagatgtt tgtagccgcg
    44221 ttgtgactct ggaagatagc cgcaaagccc tggtcgggaa tttaaaataa tcctctattt
    44281 taagacggca taatactttt ttatgccgtt taattcttcg ttttgttacc tgcctctaac
    44341 tttgtagatc tccaaaatat attcacgttg taaattgttt aacgtcaaat ttcccataca
    44401 aagctaaggg ataatgcgta gcgttcacgt aactggagga atgaaatgga gtttttcaaa
    44461 aagacggcac ttgccgcact ggttatgggt tttagtggtg cagcattggc attacccaat
    44521 atcaccattt tagcaaccgg cgggaccatt gccggtggtg gtgactccgc aaccaaatct
    44581 aactacacag cgggtaaagt tggcgtagaa aatctggtta atgcggtgcc gcaactgaag
    44641 gacattgcga acgttaaagg cgagcaggta gtgaatatcg gctcccagga catgaacgat
    44701 aatgtctggc tgacactggc gaaaaaaatt aacaccgact gcgataagac cgacggcttc
    44761 gtcattaccc acggtaccga cacgatggaa gaaactgctt acttcctcga cctgacggtg
    44821 aaatgcgaca aaccggtggt gatggtcggc gcaatgcgtc cgtccacgtc tatgagcgca
    44881 gacggtccat tcaacctgta taacgcggta gtaactgcgg cagataaagc ctccgccaac
    44941 cgtggcgtgc tggtggtgat gaacgacacc gtgctggatg ggcgggatgt caccaaaacc
    45001 aacaccaccg acgtagcaac cttcaagtct gttaactacg gtcctctggg ttacattcac
    45061 aacggtaaga ttgactacca acgtaccccg gcacgtaagc acaccagcga tacgccattc
    45121 gatatctcta agctgaatga gctgccgaaa gtcggcatcg tttataacta cgctaacgca
    45181 tccgatcttc cggctaaagc actggtagat gcgggctatg atggcatcgt tagcgctggt
    45241 gtgggtaacg gcaacctgta taaaaccgta tttgacaccc tggcaaccgc cgcgaaaaac
    45301 ggcactgcgg tagtgcgttc ttcccgcgta ccaacgggcg caaccactca ggatgccgaa
    45361 gtggatgatg cgaaatacgg cttcgtcgcc tctggcacgc tgaacccgca aaaagcgcgc
    45421 gttctgctgc aactggctct gacgcaaacc aaagatccgc agcagatcca gcagatcttc
    45481 aatcagtact aatcgcctcg ccccggtatc gtgccggggc tttttcactt cagacttacg
    45541 tccattgcca attttcatta ccctaatgat aatcaccgga ataaattatt ccgcgcgagg
    45601 gttttcgggt gaaaaagcaa tggattgttg gcacggcgct gcttatgttg atgactggta
    45661 atgcctgggc agatggcgaa ccgccaactg aaaatatctt aaaagaccaa ttcaaaaagc
    45721 agtatcacgg cattctcaag cttgatgcta tcaccttaaa aaatcttgat gctaagggta
    45781 atcaggccac ctggtcagcg gaaggcgatg tctcttccaa tgacgatctc tatacctggg
    45841 tcggtcagtt ggcagattac gaactgctcg aacagacctg gacgaaagat aaagcggtaa
    45901 aattctcggc gatgttaacc agtaaaggaa cgcccgcgtc tggctggtcg gtgaactttt
    45961 actcttttca ggcggcagcc agcgatcgtg ggcgggtggt cgacgatatc aaaacgaata
    46021 ataaatatct gatcgtgaat agcgaagatt tcaattatcg ctttagtcag cttgaatctg
    46081 cgttgaatac ccagaagaac tcgattcctg cgctggaaaa ggaagtgaaa gcgcttgata
    46141 agcaaatggt ggcggcgcag aaagcagcgg atgcatattg gggaaaagat gcaaacggta
    46201 agcaaatgac ccgcgaagac gcctttaaaa aaattcacca acagcgtgat gagtttaata
    46261 aacagaacga tagcgaggcg ttcgcggtta aatatgacaa agaggtttat caaccggcga
    46321 ttgcggcatg tcataaacag agtgaagagt gttatgaagt gccgattcag cagaagcgag
    46381 atttcgatat caacgaacaa cggcgacaga cttttctgca atcacaaaaa cttagccgta
    46441 aattgcagga tgactgggta acgctcgaaa aagggcaata tccgttaacg atgaaagttt
    46501 cagaaataaa cagtaaaaaa gtcgcgattt tgatgaagat tgacgatatc aaccaggcta
    46561 atgaacgctg gaaaaaagat accgaacaat tgcggcgcaa tggggtgatt aagtaagttt
    46621 gatatagtgc acgatttgta gcaggatgag gcgttgaccc cgcatccggc aatcaacgtc
    46681 tgatgcgacg cttgcgcgtc ttatcagacc tacaaccgct gccacatgtc ggctgaggcg
    46741 ctcacgccgc atccggcaat caacgtctga tgcgatgctt gcgcgtctta tcagacctac
    46801 aaccgctgcc acatgtcggc tgaggcgctc acgccgcatc cggcaataca agtttactca
    46861 gccagaaaaa gctccagcag cgaatttaaa aacagcttcc catgttccgt aatctgccag
    46921 taatccgcac attcggtgag ataaccctgg gcaattgcct cgtctaactg tgggcgaatc
    46981 acatcttcgc aaagcccggt atacgcacta aactctgcgc gtggcgcggc ctccagcaag
    47041 cgaaagcggt tcataaagaa ctcaaacggc ttatctgcag cttcgacatc acgctggctt
    47101 tccagatacc ttccctgcat aaaaccacgc ggatgacgcg ttttggtggt acgcagaatg
    47161 cgcccatccg ggaaggtcac tttgccgtgc gcgccgcagc caataccgat gtagtcacca
    47221 aagcgccagt agttgagatt gtgctggcac tgataactgg gtttggcgta agcggaagtt
    47281 tcatattgct gataacccgc tgcggttaat aactgatgcc cctgttcgaa tatatcccac
    47341 agcgcgtcgt cgtccggcag taccggcggg cgtgaaccaa acagcgtatt aggttcgatg
    47401 gtcagttgat accaggaaag atgcggcgga ttcagttcaa tggcctggcg tagatcgcca
    47461 agcgcctctt ccagtgattg agccggtagt ccatgcatca aatcgaggtt aaagctacgc
    47521 agccccagcc cgctcgccag ctttgccgcg cgtttcgctt cttgcgggcc atgaatacgc
    47581 ccaagtcgtt tcagcttttc ttcgctaaaa ctctgcacac caatagagat gcggttcaca
    47641 ccagcacgct gataatcgac aaagcgatcg gcttctaccg taccagggtt cgcttccata
    47701 gtaatttctg catccgctgc cagcggcaaa cgcgcacgca cgccgtccag cagcgtgtgc
    47761 atcgccgggc cggaaagcag gctcggcgta ccaccgccaa taaagattgt ctttacttca
    47821 cggccctgag cgtaagccac atcgttgtcc agatcgttaa gcagatgctg aacataatcg
    47881 tcgtgcggca cttctccttt caacgcgtga gagttgaaat cgcagtacgg gcatttctgc
    47941 acgcaccacg ggatgtgaat gtagagactc agcggcggta atttaaccat tacgtaaagc
    48001 gtccagcagc agtttcaatg cctgaccacg gtgggaaatg gcgctctttt cttcgcgggt
    48061 cagttcggca gcggttttcc cttcggaagg aacgaagaag attggatcat aaccaaagcc
    48121 accagtgccc gccggttcgc gagtaatcac gcccggccag ctgccgtggc ataccagcgg
    48181 agtcggatct tccgcgtgac gcagatatac cagcacgcag tggaaacgcg cctgacgttg
    48241 gtcgtccggt acgtctttca gtgtttccaa cagtttttgc agattctttt gatcggtcgc
    48301 gtcttcaccg gaataacgcg cggagtaaat acccggcgcg ccacccagcg catctaccgc
    48361 cagaccagag tcgtcggcaa ttgccggtaa accggtcact tttgccgcat ggcgcgcttt
    48421 cagaatcgcg ttttcgataa aggtcaggcc ggtttcttca gcggaatcaa cgccgaggtc
    48481 tgtttgggcc acgatatcaa gaccgaagtc gctaagcagc gacgccagct cacgcacttt
    48541 accggcattg ccggttgcga ggacaacttt ttgcatagga tacctaatta attaacgccg
    48601 cgatttctgg cgggatttgt tgcggattaa tgattttaat ttgtttgtgg cggccaagtt
    48661 cgcctttttc aattaccacc tggcttttgg ctacccggaa ttgcttaccg agaaacttca
    48721 ccagatggct gttggcctgg ccgtcaaccg gcggcgcggt aatggcgact ttgacttcgt
    48781 cgccatgtaa accgacaata gaatcacggc tggctttcgg ctgaatatag agccgtaaaa
    48841 ccagaccgtc atcattaact gttacggcac tcataacgcc atccacagcc ccggcagcag
    48901 catgtttccg gttgcctgta atacttctgc gacacccata ttgatgacat acagcagcag
    48961 aacgaggatc atcggcgaga aatcaatccc acccattgcc ggtagcaggc ggcgaatcgg
    49021 gcgcagcagc ggatcggcca gctgaatcag cacgtattca atcgggctac gaccctggct
    49081 tacccagctc ataatcgcca tcaccagcag gacccagaaa atcagcaggc cgatggtttt
    49141 cagcagaatc agtaaaccag caatccagat gattggcagg aaggtcacca ctttaaacag
    49201 cacgatggct ttgataaaac tgagaatgta cgcaaccagc agcgaggcgc tatcaatggg
    49261 acccattgcc ggaataacgc ggcgcagtgg cccgataatt ggctgtgtta ccttcactac
    49321 aaactgtgag aaggggttgt aaaaatcaca atgagcccac tgcatccaga tgcgtaataa
    49381 cagcaccatg gtatacagct caatgaccgt tgaaagcagg aaagtcaacg tattcatggc
    49441 gttcctttaa ttccttattt tttagagtaa tcacgcgcac caaaaattgc agtgccgata
    49501 cgaaccatcg tgctacctgc cgcaatggcg gcttccatat cgtccgacat tcccagagag
    49561 agcgtgtcga tatgcgggta gcgcgttttc agtccggcaa atgctacagc catttggcgt
    49621 gcaacttcaa actgccttac atattctgac tcaggcgcag ggattgccat cagcccgcgc
    49681 agacgtaaac gcggtagttc agcgaccgcc gctgccagct cgtccagttc tgccagttga
    49741 atcccggact tactgttttc atcactaata ttaatttgaa tcagtacgtt aagaggggga
    49801 agttctgccg ggcgctgatc gttgaggcgg gtagcaatgc gcaaacggtc gatggtatga
    49861 caccagtcga aatgctctgc caccaggcgg cttttattag actgcaacgg gccaataaaa
    49921 tgccattcta atcctgttac gcccagttcc tgaaagtggc gaattttatc taccccttcc
    49981 tgaacgtagt tttcaccaaa ttgacgctgc ccggcatcaa tggcttctgc gatggcgctc
    50041 gcaggttttg ttttactgac tgcaagcagc gtaatttctt ctggagaacg gccgcaacgc
    50101 gttgcagccg ctgagatttt gtcccggacc tgtgccaggt tatgcgcaat atcgttcatt
    50161 ttccgaggat cttagtatga atatggaaga aattgtggcc cttagtgtaa agcataacgt
    50221 ctcggatcta cacctgtgca gcgcctggcc cgcacgatgg cgcattcgcg ggctaatgga
    50281 agctgcgccg tttgatgcgc cggacgtcga agagctactg cgggagtggc tggatgacga
    50341 tcagcgggca atattgctgg aaaatggcca gctggatttt gccgtgtcgc tggcggaaaa
    50401 ccagcggttg cgtggcagtg cgttcgcgca acggcaaggt atttctctgg cattacggtt
    50461 gttaccttcg cactgtccac agctcgaaca gcttggcgca ccaacggtat tgccggaatt
    50521 actcaagagc gagaatggcc tgattctggt gacgggggcg acggggagcg gcaaatctac
    50581 cacgctggcg gcgatggttg gctatctcaa tcaacatgcc gatgcgcata ttctgacgct
    50641 ggaagatcct gttgaatata tctatgccag ccagcgatgt ttgatccagc agcgggaaat
    50701 tggtttgcac tgtatgacgt tcgcatcggg attgcgggcc gcattgcggg aagatcctga
    50761 tgtgattttg ctcggagagc tgcgtgacag cgagacaatc cgtctggcac tgacggcggc
    50821 agaaaccggg catttggtgc tggcaacatt acatacgcgt ggtgccgcgc aggcagttga
    50881 gcgactggtg gattcatttc cggcgcagga aaaagatccc gtacgtaatc aactggcagg
    50941 tagtttacgg gccgtgttgt cacaaaagct ggaagtggat aaacaggaag gacgcgtggc
    51001 gctgtttgaa ttattgatta acactcccgc ggtggggaat ttgattcgcg aagggaaaac
    51061 ccaccagtta ccgcatgtta ttcaaaccgg gcagcaggtg gggatgttaa cgtttcagca
    51121 gagttatcag cagcgggtgg gggaagggcg tttgtgacgt tgccatgccc gatgcgcaag
    51181 tttatagggc ctataaaggc aataaatgtc tgatgcgacg ctaacgcgtc ttatcagacc
    51241 tacagccgct gccagatgtg tactggataa ggcgttcacg ccgcatccgg caagatggcg
    51301 gtaacgacta tccgccaggc ttttagatcg ccttaatatc cctgctcgaa atagctttcg
    51361 agaataataa ccgcagaggc agagtcgact ttgcctttgt tgagcgcccg atagccgccc
    51421 tgttcaaaca gaccggaacg ggcttccacg gtgctaagac gttcgtcatg gagctttact
    51481 tcaacaccga aacgaccatg aatacggttg gcaaatttac gcgctctggc agtcaatggt
    51541 tgctcggtgc cgtccatatt cagcggcaaa ccgacgatga tttcgtccgg ctgccactct
    51601 ttcagtaaac gttcgatgag gttccagtcc ggcgtaccgt cctgtgcttt aattgcaggc
    51661 aaagggcgag cagtgccggt aatgcgttgg ccgaccgcta cgccaatgct tttggtgccg
    51721 aagtcgaagg cgagtaaggt tccactcatc aggcgtgccc tgccacacca ggcatggtga
    51781 gaatatccac accaatcagt tttgccgcct cgcgccagcg gtcggcaatc ggcgttttga
    51841 acagaatatt cagatccgcc ggggccgtta gccacgcgtt atcgagaatt tcttgttcca
    51901 gttgaccttt ttcccaggag gcataaccca gagccaccaa tacgtcagac ggttgtttat
    51961 cggtgccgag cgtttccagc acatcgcggg aagtggtcat cactgtgttg tctgaaatgc
    52021 gaatgctgga agcaaaattg gagggcggag tatgcaaaat aaacccgcga tcttcagcca
    52081 gcggaccgcc gagcataacc ggtttatcca gacggattga ttcatcacgc ggctccggcg
    52141 tgatcttcag cttttccaga atcccttcaa ttttgagatt ttccagcggc ttgttgacga
    52201 tgatccccat tgcaccattg gtattatgtt cgcaaatgta gaccacggaa cgacggaaaa
    52261 tcggatcctg gagagcaggc atggcaataa gaaagtgatg ctgtaaattc attgtcagag
    52321 gttctgtttc ctggttcaaa aaagcaacag cgcccagtat gcgtggaaag aagggcgctg
    52381 tcactcagag tctcaacgag atccttctcg ctaaggtggg ttactgctgc tgtaaacgtg
    52441 cttcgatggc atccattaac attccggtga tcgacaccgg aaactctgct tcaatctcac
    52501 gaatacaggt tgggctggtg acgttaattt cagtcagacg gtcgccgatg atatccagac
    52561 caacaaaaat cagccctttt tctttcagcg tcggcccgat ctgacgggcg attttccagt
    52621 cactttccgt cagcggacga ggttcaccgc gaccaccggc agccagattg ccacgggttt
    52681 cgcccccctg cggaatacgc gccaggcagt acggtaccgg ctcgccatcc accaccagca
    52741 cgcgtttgtc gccatcttta atggctggca ggtaattttg cgccatgcag tagcgagtgc
    52801 catgctcagt cagggtttcg gcaatcacgc cgaggtttgg atcgccttct ttcacgcgga
    52861 aaatcgacgc gccgcccata ccgtccagcg gcttaagaat gatgtcgctg tgtttctccc
    52921 agaacgcttt tagctgcgct ttattgcgcg taaccagcgt ttctggcgtt aagtcagaga
    52981 accaggcggt aaacagtttc tcgttacagt cgcgcaggct ctgcggcttg ttaacgatca
    53041 gcgtcccttt ctcttcggca cgttccagaa tataggtcgc gtagataaac tcggtatcaa
    53101 acggcggatc tttacgcatc aggatcacat cgagatcggc cagcggcaga tcctgttcac
    53161 cgacgaatga aaaccactct tcgtagttct gcttcacgtt cagcgtgcgg gtatgggcgc
    53221 gggcttcgcc attgatcaga tacagatcgc ccatctccat atagtgaagt tcgtaaccac
    53281 gacgctgtgc ttccagcaac atagcgaaac tggaatcttt cttgatgttg atgtttgcga
    53341 tggggtccat cacgatgccg agcttgatca ttattcttct ccgttagccc aaatcgccaa
    53401 atcgtacttg tagcgcggta atggcggtga gcgcagttgt ctctgtacgc aaaacgcgag
    53461 gtcccaacag gatatcagta aattgatagc gggcagtcat ggcaatttca tctgccgata
    53521 aaccgccttc cgggccaatc agcaggcgga cgcgttcaac cggtaacggc aacgtattga
    53581 tgctgttact ggcgcgcggg tgaagattca gtttcagtcc ttcatcctgc tctgcacacc
    53641 aggcttccag atccatcgct gggcggattt ccggcacccg gttacgacca cactgctcac
    53701 aggcagcaat tgcaatcttc tgccactgct ggagcttctt gttcagacgt tcactatcca
    53761 gtttaacgcc gcagcgctca gaaaaaagtg gcgtaatgag gcttacaccg agttcgatcg
    53821 atttctggat cgtaaattcc attttttcac cacgcgacat cacctgaccg aggtgaatat
    53881 gcagcggaga ttcgcgatcg tcgatcttgc cttccagcac cttcacttcc acgctttttt
    53941 tgctggcgct ggtaatttcg gcgtcaaaga cctggttgct accgtcaaac aattgcaacg
    54001 cctgccccgg ccccatgcgc agtacgcgcc cgatatggtt ggcggcatct tcgcaaagcg
    54061 cgatgtgaga atggctggtc agtggttcag gatgataaat gcgggggata cgcatagtta
    54121 aaaatccgcg tcgtctcccc acgcggttgt acgcgtgggg taggggttaa caaaaagagt
    54181 cccgctagtg taggttagct ctttcgcgcc tggcaagcgc gttgcacata cggattatga
    54241 ttgccttgca ccttcgcgat gcgttcatcg cgctcgcact cccagtcggt aaccggatac
    54301 atcttgttcc atgcgttgaa cagctgcgtt tgctggcgag agagtgtcag gttgtattgg
    54361 tcgcgcatat agaagtaggt gcgtgcaatg gcaccgcgtg cacgcgctgg tggttcggca
    54421 gctttttctt tgaaatcgac cttcatggcg cattgaccgt actggccttc gccgccattc
    54481 cactggctgt acataaagtt gccgcgatcg ccattcacct caccgactga cggctgcagg
    54541 ttatgcatat cgctttccat cttgcgatag accggatctt tagcgcagtt tttacgtcca
    54601 ccgtcctgcc agcactggcg ctggtgaccg aactgccagg cgggaacgac gtgttcccac
    54661 tctacgcggc tggctcggtt ttcatttttg cgcacctgat agccgcacga ttgcagatca
    54721 acaacgcctt ttttgccctg ccagttaatt ttacatccgc aataaaacgt accgggcgcg
    54781 tcagcgtgga cttttaccgc cgcggctttc gcctgagaaa aactattgat acctgcggcc
    54841 aacgccgggc cggaaaatgc tgcgctcagt accaccgcag caatagacaa ataacggtac
    54901 atcacttact ccgtgttaaa acgagccagc aacgtagcga aagttgtttt ggtatgcaat
    54961 cagccagatc aggaaagttc ctgatagttc agatggttat ttcgcaacca gctgttcacc
    55021 gcagtgaaca cagcgataga cggcctcgcc acgaactacg cgattatggc ggcgtacggt
    55081 aagctgatgc tcctggcact tgcagcggta ggggaaggtg ttgcgacgca cggattgcag
    55141 ttcgaactga tgcgtacgac gggcgggaac gcccagcacg ctttccatca tccacttcca
    55201 ctctttgcca tgtggcgcta cgcggccgaa atgtttccat accagcaaat gtgccagttc
    55261 gtgcggtacc acttcttcaa taaaagcttc actgttttcc aacagcaaaa cgggattgag
    55321 gcggatttca tagctttcca gccaggctgt tccggcggag gttccgcgct gggtgtaaga
    55381 gagttttggc tccgggtagt tacgccctag cttcaggttg gcctgggcga gtttttcccg
    55441 caggcggcgc ataacggcct gctggatggc gatagggaga cgggatgttt tcatagcggc
    55501 agaggataga gcgaagaaaa gatgactgca agaggtggct tcctccgcga tgggaggaag
    55561 cctgaggaga ttaatcgtga gcgccgattt cgcgcagttt acgacctttc atcagattac
    55621 gttcaatatg ttccagcgaa acgtgtttgg tttccggtac cagccacaat gtcagcagga
    55681 taaacagtac gttcagagcc gcatacaccc agaaggtgtt ggcgttaccc agcgtgttga
    55741 gcatggtcag gaacgttgcg ccaacgatca tgttggcaat ccagttggtg gcggtggagc
    55801 aggtgatgcc aaaatcgcgg cctttcagcg gctgaatttc ggagcacagt acccaaatca
    55861 gcggaccggc actcatggca aaaccgacaa taaacatcag cagcatggcg atggcgaaat
    55921 actgcgctga cggagagtga ataccgatat gcatcattgt accgagtacg cccatgccag
    55981 cagccatcac caggaagccc agcgttagcg ttggtttacg tccccagcgg tcaacaaggc
    56041 cgattgcgat aaaggtggca agtacgttgg tcaggccgac aatcacggtc ccccacattt
    56101 gctcggtagt gttggtataa cccgccagtt cgaagatttt cggcgcgtaa tacatgatga
    56161 cgttcatccc ggtgaattgc tgcattacct gcaacagtac gccaaggaac accgcgcggc
    56221 ggaagttgct gttctcttta aacagcgccc agccactctg tttaacctgc aaactttcac
    56281 ggatttcatc cagttcgcgt ttcgcttccg cgctggtgtc acgcaggcgt agcagcacgc
    56341 gttcggcatc aacaaaacgg cgtttggcgg caaaccaacg tgggctgtct ggcaggaaga
    56401 agacaccaat cagcagcaaa attgccggga tgataatcac acccagcatc cagcgccatg
    56461 caccggtgta gctgaaggcg gtatcagaaa gataagcacc gaggatcccg atagtgatca
    56521 tcaactgata catcgagatc atactgccac gaattttttc cggcgcaatt tcagagaggt
    56581 acagcggtgc ggtataagag gccacaccca ccgccagccc cagtagaacg cgggaaagaa
    56641 tcagtacttc aacgtttggc gcagccgcag agaacagcga accggcaaca aacaaaattg
    56701 cgccgatcat caggctcttt ttgcgcccga gtttaaagga gagccagccg ctgcccaccg
    56761 caccgactgc cgcaccgaac atcatggagc ttacgaccca ttcttgcgta tgcgaagtaa
    56821 tctggaattc atctgcaata aacggcagtg cgccagcaat tacaccgata tccaggccaa
    56881 agagtaatcc cgccagagcg gcaaggaagc agacgaaaaa cgtcattgcc ttgtttgacc
    56941 gcccctgttt tttagcgtca ggcatgatgc cctccaatat ggttattttt tattgtgaat
    57001 taagataggt gagtacgacg taaaaagatg tgaagcaaat cacatcagtg taatcgctta
    57061 cactaacatt tatcttagtt atggtttaag tacatgaaaa taattgagtt atatca
//
LOCUS       DAZGXZ010000014        52573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000014 DAZGXZ010000000
VERSION     DAZGXZ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.014"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..284
                     /locus_tag="ACHKJF_000977"
     CDS             <1..284
                     /locus_tag="ACHKJF_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0948381.1"
                     /translation="DNLADNGFYRVSPSCAQAGDILLCCFGSSVPNHAAIYCGNGELL
                     HHIPEQLSKRERYSEKWQRRTHSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            281..829
                     /locus_tag="ACHKJF_000978"
     CDS             281..829
                     /locus_tag="ACHKJF_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0948382.1"
                     /translation="MNTAAEAIRALSLQLPGFRRQMNEGWYRVRIAGEDTAPDQISTR
                     LHEPLSPGDVIHLVPRTEGAKSGGVFQTVLGVALVAAAIWMPGIGIAASNIMFSMGSA
                     MALGGVAQMLAPKAKTPEYRATDNGKQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRI
                     SQDISTRDEGGGGKVVVIGRQG"
     gene            1064..3934
                     /locus_tag="ACHKJF_000979"
                     /pseudo
     CDS             1064..3934
                     /locus_tag="ACHKJF_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            4447..5058
                     /locus_tag="ACHKJF_000980"
     CDS             4447..5058
                     /locus_tag="ACHKJF_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948383.1"
                     /translation="MHGMSITQPDGSVWLSPEFTPACLINKGNLAASKGALYRTSIPS
                     GKSCFFFIKSTARANMMFLHEQSGGVHTLKLHQLSGNPGTITVYAFSDMVLPHSGYGI
                     AMYNKNGEMIYHGEMMPLDAKLVNVDVQFSINMGYPCAIMPAMVGVYNYRRTDYDRPV
                     YVTMTGATGNQIYNGEWYSSNVTWDVRKSYTRQVLVIDTSKYD"
     gene            5163..5894
                     /locus_tag="ACHKJF_000981"
     CDS             5163..5894
                     /locus_tag="ACHKJF_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0948384.1"
                     /translation="MKISGFIAGFYFTGMSFFPVMSWAAKGEHTLSQGYVWLHVSGLK
                     NFVKNRSDYNRMALEQVVNDGFHSRENDNGGFSNYVGNDKPLQGINVRYRYEITDKLG
                     VMSSFTWVHSKTSAKSIADIHPAGAGEATSHVSARTDIIASYRSFLAGPVWRVNEYAS
                     LYAMAGAGMTKFSADLKISKNIKGSKCEPLGDKRYSKSKISFAGAVGGQFNLNETVVI
                     DVTYEYSGYGQWHASGFGAGIGFKF"
     gene            5969..7558
                     /locus_tag="ACHKJF_000982"
     CDS             5969..7558
                     /locus_tag="ACHKJF_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0948385.1"
                     /translation="MTVKISGVLKDGTGKAVPGCTIELKALRTTETVIVTTVAEGEPG
                     ENGSYSMDVEPGYYRVTLNTEGYPPVHVGNITVSAESAPGTLNTFLLNEETAAAYPKA
                     LVELEAAAAEILARAEAAARSAEVAEAKAANARGPKGDKGDPGPQGVQGPAGPTGATG
                     PAGPRGERGEQGIQGPVGATGPAGPRGEQGIQGPAGPVGPRGETGARGEKGDPGGPPG
                     PKGDTGAKGDAGPAGPAGEKGEPGERGPQGLQGPPGPAGERGPTGEAGPAGQPGHKGD
                     PGPAGPQGPAGERGPQGPQGVAGPVGPAGEPGAKGDKGDPGPAGPQGQPGPQGPAGET
                     GPAGEPGPRGEPGPQGLKGDRGEPGPTGPQGPAGERGPQGPQGVAGPVGPAGEPGAKG
                     DKGDPGGTELLASANTWTQPQTINGDLTVTGNGSFNDVQIRSDKRNKRNLVKLDNALD
                     RLEALTGYLYEIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNYNGI
                     IALLVEGFKTLRHEIKELREK"
     gene            7644..8156
                     /locus_tag="ACHKJF_000983"
     CDS             7644..8156
                     /locus_tag="ACHKJF_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948386.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLDKPFMMSQMVGRAM
                     GCKIETAYYKWNSSDQVENWGAVGADWPLEEKSKGTITNAENCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVTQDLKIS"
     gene            8229..8444
                     /locus_tag="ACHKJF_000984"
                     /pseudo
     CDS             8229..8444
                     /locus_tag="ACHKJF_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(8708..8956)
                     /locus_tag="ACHKJF_000985"
     CDS             complement(8708..8956)
                     /locus_tag="ACHKJF_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0948387.1"
                     /translation="MRVEICIAKEKISKMPKGSVPALQQEMLRQISKRYDDVEVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQNVWETADDWFIH"
     gene            complement(9182..9277)
                     /locus_tag="ACHKJF_000986"
                     /pseudo
     CDS             complement(9182..9277)
                     /locus_tag="ACHKJF_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9274..9840)
                     /gene="yecD"
                     /locus_tag="ACHKJF_000987"
     CDS             complement(9274..9840)
                     /gene="yecD"
                     /locus_tag="ACHKJF_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0948388.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            10150..11922
                     /gene="aspS"
                     /locus_tag="ACHKJF_000988"
     CDS             10150..11922
                     /gene="aspS"
                     /locus_tag="ACHKJF_000988"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP0948389.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            12040..12492
                     /gene="nudB"
                     /locus_tag="ACHKJF_000989"
     CDS             12040..12492
                     /gene="nudB"
                     /locus_tag="ACHKJF_000989"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP0948390.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            12521..13261
                     /gene="yebC"
                     /locus_tag="ACHKJF_000990"
     CDS             12521..13261
                     /gene="yebC"
                     /locus_tag="ACHKJF_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0948391.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            13296..13817
                     /gene="ruvC"
                     /locus_tag="ACHKJF_000991"
     CDS             13296..13817
                     /gene="ruvC"
                     /locus_tag="ACHKJF_000991"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP0948392.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(13819..14421)
                     /gene="yebB"
                     /locus_tag="ACHKJF_000992"
     CDS             complement(13819..14421)
                     /gene="yebB"
                     /locus_tag="ACHKJF_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP0948393.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            14492..14557
                     /gene="yobI"
                     /locus_tag="ACHKJF_000993"
     CDS             14492..14557
                     /gene="yobI"
                     /locus_tag="ACHKJF_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFP0948394.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            14696..15307
                     /gene="ruvA"
                     /locus_tag="ACHKJF_000994"
     CDS             14696..15307
                     /gene="ruvA"
                     /locus_tag="ACHKJF_000994"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP0948395.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPTTDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            15316..16326
                     /gene="ruvB"
                     /locus_tag="ACHKJF_000995"
     CDS             15316..16326
                     /gene="ruvB"
                     /locus_tag="ACHKJF_000995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP0948396.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(16473..17258)
                     /gene="znuB"
                     /locus_tag="ACHKJF_000996"
     CDS             complement(16473..17258)
                     /gene="znuB"
                     /locus_tag="ACHKJF_000996"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP0948397.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(17255..18010)
                     /gene="znuC"
                     /locus_tag="ACHKJF_000997"
     CDS             complement(17255..18010)
                     /gene="znuC"
                     /locus_tag="ACHKJF_000997"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP0948398.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            18089..19021
                     /gene="znuA"
                     /locus_tag="ACHKJF_000998"
     CDS             18089..19021
                     /gene="znuA"
                     /locus_tag="ACHKJF_000998"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP0948399.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            19037..20359
                     /gene="mepM"
                     /locus_tag="ACHKJF_000999"
     CDS             19037..20359
                     /gene="mepM"
                     /locus_tag="ACHKJF_000999"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP0948400.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            20479..21450
                     /gene="lpxM"
                     /locus_tag="ACHKJF_001000"
                     /gene_synonym="msbB"
     CDS             20479..21450
                     /gene="lpxM"
                     /locus_tag="ACHKJF_001000"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0948401.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(21582..23024)
                     /gene="pyk"
                     /locus_tag="ACHKJF_001001"
     CDS             complement(21582..23024)
                     /gene="pyk"
                     /locus_tag="ACHKJF_001001"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP0948402.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(23152..24021)
                     /gene="hexR"
                     /locus_tag="ACHKJF_001002"
                     /gene_synonym="yebK"
     CDS             complement(23152..24021)
                     /gene="hexR"
                     /locus_tag="ACHKJF_001002"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP0948403.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            24359..25834
                     /gene="zwf"
                     /locus_tag="ACHKJF_001003"
     CDS             24359..25834
                     /gene="zwf"
                     /locus_tag="ACHKJF_001003"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP0948404.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDKDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            26069..27880
                     /gene="edd"
                     /locus_tag="ACHKJF_001004"
     CDS             26069..27880
                     /gene="edd"
                     /locus_tag="ACHKJF_001004"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP0948405.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            27917..28558
                     /gene="kdgA"
                     /locus_tag="ACHKJF_001005"
                     /gene_synonym="eda"
     CDS             27917..28558
                     /gene="kdgA"
                     /locus_tag="ACHKJF_001005"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0948406.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(28614..29792)
                     /gene="purT"
                     /locus_tag="ACHKJF_001006"
     CDS             complement(28614..29792)
                     /gene="purT"
                     /locus_tag="ACHKJF_001006"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP0948407.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLSTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            29926..30216
                     /gene="yebG"
                     /locus_tag="ACHKJF_001007"
     CDS             29926..30216
                     /gene="yebG"
                     /locus_tag="ACHKJF_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP0948408.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            30283..30639
                     /gene="yebF"
                     /locus_tag="ACHKJF_001008"
     CDS             30283..30639
                     /gene="yebF"
                     /locus_tag="ACHKJF_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP0948409.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            30965..31624
                     /gene="yebE"
                     /locus_tag="ACHKJF_001009"
     CDS             30965..31624
                     /gene="yebE"
                     /locus_tag="ACHKJF_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0948410.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            31833..33893
                     /gene="ptrB"
                     /locus_tag="ACHKJF_001010"
     CDS             31833..33893
                     /gene="ptrB"
                     /locus_tag="ACHKJF_001010"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP0948411.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPESETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLGYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(33890..34552)
                     /gene="exoX"
                     /locus_tag="ACHKJF_001011"
     CDS             complement(33890..34552)
                     /gene="exoX"
                     /locus_tag="ACHKJF_001011"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP0948412.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(34576..35232)
                     /gene="yobB"
                     /locus_tag="ACHKJF_001012"
     CDS             complement(34576..35232)
                     /gene="yobB"
                     /locus_tag="ACHKJF_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP0948413.1"
                     /translation="MSFWKVATAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(35334..35564)
                     /gene="holE"
                     /locus_tag="ACHKJF_001013"
     CDS             complement(35334..35564)
                     /gene="holE"
                     /locus_tag="ACHKJF_001013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP0948414.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            35703..36077
                     /gene="yobA"
                     /locus_tag="ACHKJF_001014"
     CDS             35703..36077
                     /gene="yobA"
                     /locus_tag="ACHKJF_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP0948415.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            36081..36953
                     /gene="copD"
                     /locus_tag="ACHKJF_001015"
     CDS             36081..36953
                     /gene="copD"
                     /locus_tag="ACHKJF_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP0948416.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            36966..37307
                     /gene="yebY"
                     /locus_tag="ACHKJF_001016"
     CDS             36966..37307
                     /gene="yebY"
                     /locus_tag="ACHKJF_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP0948417.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            37703..38359
                     /gene="pphA"
                     /locus_tag="ACHKJF_001017"
     CDS             37703..38359
                     /gene="pphA"
                     /locus_tag="ACHKJF_001017"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0948418.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(38360..38551)
                     /gene="yebW"
                     /locus_tag="ACHKJF_001018"
     CDS             complement(38360..38551)
                     /gene="yebW"
                     /locus_tag="ACHKJF_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP0948419.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(38656..38892)
                     /gene="yebV"
                     /locus_tag="ACHKJF_001019"
     CDS             complement(38656..38892)
                     /gene="yebV"
                     /locus_tag="ACHKJF_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP0948420.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(39010..40449)
                     /gene="rsmF"
                     /locus_tag="ACHKJF_001020"
     CDS             complement(39010..40449)
                     /gene="rsmF"
                     /locus_tag="ACHKJF_001020"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP0948421.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(40529..43162)
                     /gene="yebT"
                     /locus_tag="ACHKJF_001021"
     CDS             complement(40529..43162)
                     /gene="yebT"
                     /locus_tag="ACHKJF_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP0948422.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLRPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVTGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFKQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(43131..44414)
                     /gene="yebS"
                     /locus_tag="ACHKJF_001022"
     CDS             complement(43131..44414)
                     /gene="yebS"
                     /locus_tag="ACHKJF_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP0948423.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            44544..45041
                     /gene="msrC"
                     /locus_tag="ACHKJF_001023"
     CDS             44544..45041
                     /gene="msrC"
                     /locus_tag="ACHKJF_001023"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP0948424.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            45138..45836
                     /gene="proQ"
                     /locus_tag="ACHKJF_001024"
     CDS             45138..45836
                     /gene="proQ"
                     /locus_tag="ACHKJF_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP0948425.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            45856..47904
                     /gene="prc"
                     /locus_tag="ACHKJF_001025"
     CDS             45856..47904
                     /gene="prc"
                     /locus_tag="ACHKJF_001025"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP0948426.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            48096..48977
                     /gene="htpX"
                     /locus_tag="ACHKJF_001026"
     CDS             48096..48977
                     /gene="htpX"
                     /locus_tag="ACHKJF_001026"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP0948427.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(49022..50395)
                     /gene="yebQ"
                     /locus_tag="ACHKJF_001027"
     CDS             complement(49022..50395)
                     /gene="yebQ"
                     /locus_tag="ACHKJF_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0948428.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTMLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LQGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            50572..51363
                     /gene="kdgR"
                     /locus_tag="ACHKJF_001028"
     CDS             50572..51363
                     /gene="kdgR"
                     /locus_tag="ACHKJF_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP0948429.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(51506..51745)
                     /gene="yobH"
                     /locus_tag="ACHKJF_001029"
     CDS             complement(51506..51745)
                     /gene="yobH"
                     /locus_tag="ACHKJF_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP0948430.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            51904..52047
                     /gene="mgrB"
                     /locus_tag="ACHKJF_001030"
     CDS             51904..52047
                     /gene="mgrB"
                     /locus_tag="ACHKJF_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP0948431.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            52122..52409
                     /gene="yebO"
                     /locus_tag="ACHKJF_001031"
     CDS             52122..52409
                     /gene="yebO"
                     /locus_tag="ACHKJF_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP0948432.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
BASE COUNT        13387 a        12760 c        13985 g        12441 t
ORIGIN      
        1 tggacaacct ggcagacaac ggcttttacc gggtgtctcc gtcctgtgca caggcaggcg
       61 atatcctgct gtgctgcttt ggttcatcgg tgccgaatca tgccgccatt tactgtggca
      121 acggtgaact gcttcaccat atacctgaac aactgagtaa acgggagagg tattcagaga
      181 aatggcaacg acgaacgcat tctgtctggc gtcaccgcca ctggtccgca tctgccttca
      241 cggggattta caacgatttg gtcgccgcat cagcctgtat gtgaacacgg cagcagaggc
      301 catccgtgcc ctgtcgctgc agctgccggg attccgccgt cagatgaacg aaggctggta
      361 ccgggtacgt attgccggtg aggatactgc gccggaccag atatcgaccc gactgcatga
      421 gccactgagc ccgggggatg tcattcatct ggtaccccgg actgaaggag ccaaaagtgg
      481 cggtgtgttt cagactgtgc tgggcgttgc gctggttgca gctgctatct ggatgccggg
      541 tatcggtatt gcagccagca atattatgtt ttccatgggt tctgcaatgg cgctgggtgg
      601 tgtggctcag atgctggccc cgaaggcaaa aacaccggag tacagggcga cggataacgg
      661 taaacagaac acgtattttt cctcactgga taacatgatt gcccagggga acccgatgcc
      721 ggtgccttac ggtgaaatgc tggttggctc ccggcgaatc tcccaggaca tcagcacccg
      781 tgatgaaggt ggtggcggga aggtggtggt tatcgggcgg caggggtaaa gcataaaaaa
      841 aaagtcccgc agtgttgcgg agctgcggga gagaacgatg aagattaacg ttatggagtt
      901 atttttcagg cactaaaaag taacgcagcg tatttatcag cgcaacagtc ttttttttca
      961 gaatgtgaag aaattcagaa tttttattca gtcatgatac aggcatcctc cgggatgcct
     1021 gttgtttttg tgcgtaacag ttatcgcagt aaagggtgag acaatgggca aaggtggcgg
     1081 caaggggcac acgccggttg aggcaaagga caatctcaaa tccacgcaga tgatgagcgt
     1141 gattgatgcg attggtgagg gaccgataga aggcccggtg aaggggctgc agagtattct
     1201 ggtgaacaaa accccgctga cggacacgga cggtaatccc gtgatacacg gtgtgaccgc
     1261 ggtctggcgt gccggggagc aggagcagac accacccgaa ggctttgagt cctccggagc
     1321 tgaaaccgca ctgggcgtgg aagtgacgaa ggcaaagccg gtgacgcgca ccattacgtc
     1381 cgcgaacatt gaccgcctgc gggtcacctt cggggtgcag tcactggtgc agaccacgtc
     1441 agagggtgac cgaaacccgg catccgtccg cctgctgatt cagttacagc gtaacggtaa
     1501 ctgggtgacg gaaaaggatg tcaccattaa tggcaagacc acctcgcagt ttctggcgtc
     1561 ggtgattctg gataacctgc ctgagcgtcc ttttaacatc cggatggtcc gggagacggc
     1621 ggacagcacc acggaccagc tgcagaacag aacgctgtgg tcgtcataca ccgaaatcat
     1681 cgatgtgaaa cagtgctacc cgaacacggc gattgtgggc ctgcaggtgg atgcggagca
     1741 gtttggcggt cagcagatga cggtgaacta ccatatccgc ggtcgcatca tccaggtgcc
     1801 gtcaaactat gacccggaaa aacgcactta cagcggcatc tgggacggca gcctgaaacc
     1861 ggcatacagc aacaacccgg cctggtgcct gtgggacatg ctgacccacc cgcgttacgg
     1921 aatgggaaaa cgccaggggg cggcggatgt ggacaaatgg gcgctgtatg ccatcgggca
     1981 gtactgcgac cagatggtcc cggatggttt cgggggcaca gagccgcgga tgacctttaa
     2041 tgcatacctg tcacaacagc gtaaggcgtg ggacgtcctc agtgatttct gctcggcgat
     2101 gcgctgtatg ccggtatgga acggccagac gctgacgttc gttcaggacc gctcgtcgga
     2161 tgtggtgtgg ccgtacacca acagcgatgt ggtggtggat gataacggcg tggggttccg
     2221 ctacagcttc agtgccctga aggaccgtca cacggcggtg gaggtgaatt acaccgaccc
     2281 gcagaacggc tggcagacct ccacggaact ggtggaagac ccggacgcca tactgcgcta
     2341 cggacgcaac ctgctgaaga tggatgcgtt cggctgcacc agtcgcggtc aggcccaccg
     2401 tgccgggctg tgggtgataa agaccgaact gctggaaacg cagacggtgg atttcacgct
     2461 cgggtcacag gggctgcgtc acacacccgg tgacattatt gaaatctgtg ataacgacta
     2521 tgccgggacc ctgaccggcg gacgcatcct gtccattgat gccgccagcc gcaccctgac
     2581 actggaccgt gaggtgaccc tgccggagac aggtacttcg acggtgaacc tgattaacgg
     2641 cagcggtaag ccggtgagcg tggacatcac tgcacacccc gcgccggacc ggatacaggt
     2701 cagtaccctg ccggatggtg tggagacata cggggtgtgg ggactctccc tgccgtcact
     2761 gcgtcgtcgc ctgttccgct gtgtctccat ccgggaaaac acggacggca cctttgccat
     2821 cacggcagtg cagcatgtgc cggaaaaaga agccatcgtg gataacgggg ccagctttga
     2881 gccgcagtca ggcaccctga acagcgttat cccaccggca gtgcagcacc tcacggtgga
     2941 ggtgagcgcg gctgacggcc agtatctggc acaggcgaaa tgggacacgc cgcgggtggt
     3001 gaagggggtg cgcttcagtc tgcgactgac cagcggaagc ggagaagaca gccgtctggt
     3061 gaccaccgcc atcactgcgg atacagagca tcgtttcagt ggtctgccgc tcggggaata
     3121 caccctgacg gtcagggcga ttaacagcta tggccagcag ggcgaaccgg ccaccaccac
     3181 cttccggatt aacgcgccag caaaacccgc caccattgaa ctgacgccgg ggtattttca
     3241 gataacggcg gtcccgcgtc ttgcggtgta tgacccgacg gtacagtttg aattctggtt
     3301 ttcggaggca aaaatcgcag acaccgcaca ggtggaaacc gctgcccgtt atctggggac
     3361 cggcagccag tggagcgtct ccggcagccg gattaagccg gggacggatt tctggtttta
     3421 tgtgcgcagc gtcaacctgg tgggcaagtc tgcgtttgtg gaagccggcg ggcaggccag
     3481 caatgatgcg gaaggttatc tggacttttt cagaggagaa atagggaaaa cgcatctggc
     3541 tcaggggctg tgggagctga tagataacag ccagcttgcg gatgatatgg cggaaatgca
     3601 gaccaccatc accgaaaccc gcaatgaaat cacacagacg gtcagtaaaa cgctggagag
     3661 ccagagcgcc accatacagc agatacagcg cgtgcagacg ggcacaaatg atgacctggc
     3721 tgcactctac atgctgaagg tacagaaaac gaaagacggc attccctatg tggccgggat
     3781 aggtgcgggg attgaggata ctgatggtca gacgctgagc aacatactgc tgcaggctga
     3841 ccgtatagcg atgattaacc cgcaggacgg caacaccacg ccgttatttg tggcgcaggg
     3901 gaatcagttg ttcctgaatg atgttctgat gaaatcgctg attgttgatt ttgccagtat
     3961 tacaggtgag atccagtctg atggttttaa atccggatca cccggctggc gtttttcacg
     4021 taacggaaaa ctggaaatta acagcagtaa tgacggtggg cgcatgactt ttaacggcga
     4081 tcgtatcgat gtgtatgatc agaatggtgt gctgcgtgtc cggatgggga aactctgatg
     4141 ttcattcttc aggagcagtg atatgggata cggttattct gtttatgatg gcaaaggaag
     4201 ggatatcagc gggtttctca ctccgatatt ttttcttgac agattcacgg catcgtcagg
     4261 ctcaaaaaca tacgcagatc caccacgggg aaagtcattg tgtgctgtat gttcattaat
     4321 gcctttgaat aatgataatc atataaatat tccgtctcct cagattgtta ttgaaggtaa
     4381 cacggttcgc tggtcaaacc tgtatcaggg attaggttca tatatttata cattctggag
     4441 gtgagcatgc atggaatgag tattacacag ccggatggaa gtgtatggct gagcccggaa
     4501 tttacccctg cctgtttaat taataagggg aatctggcag cctcaaaggg cgcgctatac
     4561 agaacatcaa taccttccgg gaagtcgtgt tttttcttta taaaaagcac cgccagggca
     4621 aatatgatgt ttttacatga acagtctggt ggtgtgcata cattaaaact ccatcagtta
     4681 tcaggtaacc caggaacaat tactgtttat gcgttttccg atatggtgtt accgcattct
     4741 ggttatggta ttgccatgta taataaaaac ggagagatga tttatcatgg cgagatgatg
     4801 ccgcttgatg caaaactggt taatgttgat gtccagtttt caattaacat ggggtatccg
     4861 tgtgcaataa tgcctgcaat ggttggcgta tataattaca gacgaacaga ttatgacagg
     4921 cctgtatatg tcaccatgac gggggcaacg ggaaatcaga tatataacgg ggaatggtat
     4981 tcgagtaatg tcacctggga tgtgcgtaaa tcctatacca gacaggttct tgttattgat
     5041 acatcaaaat atgattaaat cactgtgcag atagtttatt ttgatggtca gttaatttta
     5101 tgcgtaaaca gcatgtgatg cattatcgaa tctgttctgt ttaattatgg ctgggtatta
     5161 aaatgaaaat atcaggtttt attgccgggt tttattttac tggcatgagt ttctttcccg
     5221 tgatgtcgtg ggctgcaaag ggggagcata cgttgtcgca ggggtatgtc tggcttcatg
     5281 tgtccggtct gaaaaatttt gtgaaaaaca ggtctgatta taacagaatg gcacttgaac
     5341 aggttgtaaa tgacggtttt cattcccggg aaaatgataa tggtggtttc agcaattatg
     5401 tgggaaacga taagccactg caggggataa acgtcaggta tcgttatgaa ataacggata
     5461 agctgggtgt gatgagttct tttacctggg tacactcaaa aaccagtgca aaatccatag
     5521 ctgatattca tcccgccggg gcaggagagg caacatccca tgtatcagcc aggactgata
     5581 tcattgccag ttaccggagt ttcctggcag ggccggtatg gcgggttaat gagtatgcca
     5641 gtctctatgc aatggctggt gctggcatga caaaattttc tgcggatttg aaaatcagta
     5701 aaaatatcaa aggcagtaaa tgtgaaccat tgggggataa acgttacagc aaaagtaaaa
     5761 tatctttcgc cggggcggtg ggagggcagt ttaacctgaa cgaaaccgtt gttattgatg
     5821 tgacatacga atattcaggt tatggtcagt ggcatgccag tggctttggt gcaggcatcg
     5881 gttttaaatt ctgacggcca aaatacgacg gttttagtct gcccgtagca tcattgctgc
     5941 gggctttttt tattattggt ggaagagtat gacagtaaaa atttctggcg tgcttaaaga
     6001 tggcaccggc aaagcggtac ctggatgcac gattgaactc aaagccctgc ggacgacgga
     6061 gacggtgatt gtcaccaccg tggcagaggg agagccggga gaaaacggca gttacagtat
     6121 ggatgtggag ccggggtatt accgtgtgac gctgaacaca gaggggtatc caccggtgca
     6181 cgtgggaaac atcaccgtca gtgctgaatc agcaccgggg acactgaata cgtttttgct
     6241 gaatgaagag actgcggcgg cgtacccgaa ggcgctggtc gaactggagg ccgctgccgc
     6301 ggaaattctg gcgcgcgcgg aagcggcggc gcgtagtgcg gaggtggcgg aggcaaaggc
     6361 agcgaatgcc cgggggccaa agggagataa gggtgacccg ggacctcagg gggtacaggg
     6421 tccggcaggc ccgacaggag caaccggccc cgcggggcca cggggtgaac gtggtgaaca
     6481 gggcatacag gggccagttg gtgcaaccgg accggcgggg ccgcgaggag agcagggtat
     6541 tcagggaccg gcaggtccgg tcggtccccg aggagaaacc ggtgccagag gagaaaaggg
     6601 cgacccgggc ggcccgccag ggccgaaggg tgacacaggc gctaaaggag atgccggacc
     6661 tgctggccct gccggagaaa aaggcgaacc cggtgagcgt gggcctcagg gattacaggg
     6721 accaccgggt cctgccggag agagagggcc gacaggcgaa gctggacctg cgggtcaacc
     6781 ggggcacaaa ggagacccgg gtcctgctgg cccacaggga ccggcgggag agcggggacc
     6841 tcaggggccg cagggtgtag ctggtcctgt cggcccggca ggcgaaccgg gagcgaaagg
     6901 cgataaaggt gaccccggac ctgctggtcc tcaggggcag cccggtcctc agggacctgc
     6961 tggcgaaacc ggacccgcgg gtgaacccgg gccaagaggt gagcccggtc cacagggact
     7021 taaaggtgac aggggcgagc caggacctac aggcccacag ggaccggcgg gagagcgggg
     7081 acctcagggg ccacagggtg tagctggtcc tgtcggcccg gcaggcgaac cgggagcgaa
     7141 aggcgataaa ggtgaccccg gaggaaccga actgcttgct tcagccaata catggactca
     7201 accacaaact attaatggcg atttgacggt aaccggaaac ggctcattta acgacgttca
     7261 gatccgctcg gataaacgca acaagcgaaa tctggtaaaa ctggataatg cgttagatcg
     7321 tctggaggca cttactggtt atctttacga gatacagtac tctgccgacg gttggcaaac
     7381 gtcggttggt ttaattgctc aggatgcaca aaaagcattg cctgaactgg taactgaaga
     7441 cgcagacgtt atatctggtg aaaaacgtct gcgtcttaac tacaacggca taattgcatt
     7501 gttagtcgaa ggctttaaaa cacttcgtca tgagattaaa gaactccggg agaagtgaac
     7561 gacagctgtt gtagtttctg gtttctactg aatttaaatt gtggggtgac actcacccca
     7621 cgcattcaga agggggagat gagatggggg taacatcggg atgggtaggc tcttcggcta
     7681 agagcgaaac aggtgagcag tggatgggag ccgccggaac caaactggga ttggataaac
     7741 cttttatgat gagtcaaatg gtggggcgag ctatgggttg taaaatagaa accgcttact
     7801 ataaatggaa ctcttcggat caagttgaaa actggggggc ggttggagct gattggccat
     7861 tagaagaaaa aagcaaaggg acaattacaa acgctgaaaa ctgtggttct gggagactgg
     7921 tgggggctgt cgttacactt tctcactttt tgacgaactc tacaccgaca gctgctgttt
     7981 atttagccgg tggtaaagca ggtaacatca ccgtaaacgt aggtggtgcc acacaaacca
     8041 tgatttatca gggcgttgtt agtgggttcc agtattactg gtcaggttct gttagttccg
     8101 ctttcgtgga ggcaatgaaa aagacgggag taacccagga tctaaaaatt agttaaatgg
     8161 taaatgaata attttaaaaa cttcacgttc tacataccgg agacgccgga tatataggat
     8221 attgttttaa gttgccagag aaatttttcc ggacggatgc tgataataat gatgtgccaa
     8281 tgacggcagc atcattgatg gcactgagtg aagcggcaga gcaggcgatg ttcagaaaag
     8341 ggatggaaat ccacgtaagg cagcgcacca tgaagaagga gctggaaaag ctgagcagtg
     8401 cggatgagat actggcatac agggttggct gggtgcagga ataacagaca aaaagatggg
     8461 gagcatcacc tcccccatga actgagtcaa ttatacactt gtgaagtatt tactatgcga
     8521 ataaatactg tcacggaaag tgcaggcctg gatgtgatgg aaatcatgtt tccggcgcat
     8581 cctgttgatg tggatgattt ttcatatttc cggaagtccg tattttgtcc gggcgcggtt
     8641 gcatgcttca ttcgcgatgc catttttgcc ggatatagcg agttttctgc atgttgacgt
     8701 tttttagtca gtgaataaac cagtcgtccg ctgtttccca gacattttga agcgtttctt
     8761 gtacaaactc ttgcgcggat tctttatcca cggctcttaa aatagtgagt ccatcattgc
     8821 tggcagattt aacgatcact tctacatcgt cataacgctt actgatttgt cgcagcattt
     8881 cttgctgtag ggcaggaaca gaaccttttg gcattttgct gattttttct ttagcaatac
     8941 agatctcaac acgcataaca ccctcctgta actgtggtta tatacaggtg ttatttttat
     9001 ctgtatggat aaccagtgtc aattcctgat attgtttatg gggcatcaat ggggcatgta
     9061 tgggacactt tttatcggcg aaattcgtcg aagttcgtcg acatggtaaa cgaatcatct
     9121 atccaaccct tgaaaaacgg cgctcctgga cgatcttcgt cgatttttaa aaatgttgcg
     9181 tcacgcagtt aaagtggcgg gcatactctt caaggctggt gatccccaac cgcacccatt
     9241 taggatgcga ccattgcggt agaccgatgt aaatcataac gcgtgaagga tctcttccac
     9301 gctacgcaca cgggcgatgc gtgggtagat atggttgatg ctgttattgt gctgctcggc
     9361 gctggcggca ctacaggcgt cttcggcaat caccagatta aagccaagtt cccaggcatt
     9421 gcgggcggtg gattcaacac cgatattggt cgagatccca cataacacta ttgtatcgat
     9481 acctcggcgg cgtaattgca actccagatc cgtaccgtaa aacgcacccc actgacgttt
     9541 gatgatttcg atatcactgt cagttgcacc taatgcagct ggatgttgcc accagttttc
     9601 gggcaacact ttagcggggg agggggcatc aaccggctgt tttaatgctt cggcgtaatc
     9661 ggcagaccag ccaactcgca ccagaaacac gggctgaccg ctggcgcgaa atttcgccgc
     9721 cagcttcccg gcgcgattaa ctacctcatc ggcagtatgt ggaccaccgg caaaaggtaa
     9781 gatgccttct tgtaaatcga tcaccaccaa cgcagtggtt ttagcattaa gttcaagcat
     9841 gctgactcct gaaaaaacaa aatcgtgcct cacaccttaa tgcgattagc cgatggcgga
     9901 acgacaaatt ttgttaattt ttatgagttc gggcaacacc tttatagcaa actggcgtag
     9961 agtctgattt caggcccgat ataaagtggt aacgaggcgt tccagcgcgg gccaggctct
    10021 tacggtgcgg cagaatttcc agtataatag ccgccttttt tcattcagtt gtgacataca
    10081 gctaacgctg cgacttggtc acctgcgcag cagggggcat ccgccggcag ctaagttaag
    10141 ggatatctca tgcgtacaga atattgtgga cagctccgtt tgtcccacgt ggggcagcag
    10201 gtgactctgt gtggttgggt caaccgtcgt cgtgatcttg gtagcctgat cttcatcgat
    10261 atgcgcgacc gcgaaggtat cgtgcaggta tttttcgatc cggatcgtgc ggacgcgtta
    10321 aagctggcct ctgaactgcg taatgagttc tgcattcagg tcacgggcac cgtacgtgcg
    10381 cgtgacgaaa aaaatattaa ccgcgatatg gcgaccggcg aaatcgaagt gctggcgtcc
    10441 tcgctgacta tcatcaaccg cgcagatgtt ctgccgcttg actctaacca cgtcaacacc
    10501 gaagaagcgc gtctgaaata ccgctacctc gacctgcgtc gtccggaaat ggctcagcgc
    10561 ctgaaaaccc gcgctaaaat caccagcctg gtgcgccgtt ttatggatga ccacggcttc
    10621 ctcgacatcg aaactccgat gctgaccaaa gccacgccgg aaggcgcgcg tgactacctg
    10681 gtgccttctc gtgtgcacaa aggtaaattc tacgcactgc cgcaatcccc acagttgttc
    10741 aaacagctgc tgatgatgtc cggttttgac cgttactatc agatcgttaa atgcttccgt
    10801 gacgaagacc tgcgcgctga tcgtcagcct gaatttactc agatcgatgt ggaaacttct
    10861 ttcatgaccg cgccgcaagt gcgtgaagtg atggaagcgc tggtgcgtca tttgtggctg
    10921 gaagtgaagg gcgtggatct gggcgatttc ccggtaatga cctttgcgga agcagaacgc
    10981 cgttatggtt ctgataaacc ggatctgcgt aacccgatgg aactgaccga cgttgctgat
    11041 ctgctgaaat ctgttgagtt tgcagtattt gcaggtccgg cgaacgatcc gaaaggtcgc
    11101 gttgccgctc tgcgcgttcc gggcggcgca tcgctgaccc gtaagcagat cgacgaatac
    11161 ggtaacttcg ttaaaatcta cggcgcgaaa ggtctggctt acatcaaagt taacgaacgc
    11221 gcgaaaggtc tggaaggtat caacagcccg gtagcgaagt tccttaatgc agaaatcatc
    11281 gaagccatcc tggatcgtac tgccgcgcaa gatggcgata tgattttctt cggtgccgac
    11341 aacaagaaaa ttgttgccga cgcgatgggc gcactgcgcc tgaaagtggg taaagacctt
    11401 ggtctgaccg acgaaagcaa atgggcaccg ctgtgggtta tcgacttccc gatgtttgaa
    11461 gacgacggtg aaggcggcct gacggcaatg caccatccgt tcacctcacc gaaagacatg
    11521 actgctgcag aactgaaagc tgcaccggaa aatgcggtgg cgaacgctta cgatatggtc
    11581 atcaatggtt acgaagtggg cggtggttca gtacgtatcc ataatggtga tatgcagcag
    11641 acggtgtttg gtattctggg tatcaacgaa gaggaacagc gcgagaaatt cggcttcctg
    11701 ctcgacgctc tgaaatacgg tactccgccg cacgcaggtc tggcattcgg tcttgaccgt
    11761 ctgaccatgc tgctgaccgg caccgacaat atccgtgacg ttattgcctt cccgaaaacc
    11821 acggcggcag cgtgtctgat gactgaagca ccgagctttg ctaacccgac tgcactggct
    11881 gagctgagca ttcaggttgt gaagaaggct gagaataact gatatgactc aaatacacga
    11941 aatcattcgc gttgcatcaa ggcggcaact gagtgaactc ccatgagcat agataactat
    12001 gtgaatggga tgagcgaagg cagtcaacga agaggcagcg tgaaggataa agtgtataag
    12061 cgtcccgttt cgatcttagt ggtcatctat gcacaagata cgaaacgggt gctgatgttg
    12121 cagcggcgtg acgatcccga tttctggcag tcggtaaccg gcagcgtgga agagggtgaa
    12181 accgcgccgc aagctgccat gcgcgaagta aaggaagagg tcaccattga tgttgtcgct
    12241 gaacaactga ccttaattga ctgtcagcgc acggtagagt ttgaaatttt ttcacattta
    12301 cgtcatcgct atgcgccggg cgtgacgcgt aatacggaat catggttctg tcttgcgctt
    12361 ccgcacgagc ggcagatcgt tttcactgaa catctggctt acaagtggct tgatgcgcct
    12421 gctgcggcgg cgctcactaa gtcctggagc aaccggcagg cgattgaaca gtttgtcatt
    12481 aacgctgcct gaacaggcag ggctatctgg agatattttt atggcaggtc atagtaaatg
    12541 ggccaacacc agacatcgta aagctgcgca ggatgcaaag cgcggtaaaa tcttcactaa
    12601 aatcattcgt gagctggtaa ccgcggctaa gctgggcggt ggcgatccgg acgctaaccc
    12661 gcgtctgcgt gcagcaattg ataaagcact gtctaacaac atgacccgcg acacactgaa
    12721 ccgcgcaatt gcacgtggtg tgggcggtga tgatgatgca aacatggaaa ccatcatcta
    12781 cgaaggttac ggtcctggcg gcacggcaat catgattgaa tgtctgtctg acaaccgcaa
    12841 ccgtaccgtt gctgaagtgc gtcatgcatt cagcaaatgt ggcggtaacc tcggtactga
    12901 tggttccgta gcctatctgt tcagcaaaaa aggcgtgatc tccttcgaga aaggcgatga
    12961 agacaccatc atggaagcag ctttggaagc aggtgctgaa gacgttgtga cctatgatga
    13021 cggcgcgatt gatgtctaca ccgcatggga agaaatgggt aaagtgcgcg acgctctgga
    13081 agcggcaggt ctgaaagcag acagcgcgga agtttccatg atcccgtcta ccaaagctga
    13141 tatggatgca gaaaccgcac cgaaactgat gcgtctgatc gatatgctgg aagattgcga
    13201 cgacgtgcag gaagtttacc ataacggtga aatctctgat gaggttgcag cgactctctg
    13261 atgaggcctg ctaaacagca aaacggagac gcgtgatggc tattattctc ggcattgatc
    13321 cgggttcgcg cgtgaccggc tatggcgtca tccgccaggt aggcaggcaa ctgtcctacc
    13381 tgggtagtgg atgcatacgc accaaagtgg atgatttacc gtctcgtctg aagctcatct
    13441 atgcgggcgt gacggaaatc atcacccagt tccagcctga ttatttcgcc attgaacaag
    13501 tctttatggc gaagaacgct gactcagccc tgaaactggg ccaggcgcgc ggcgtggcga
    13561 ttgtggcggc ggtgaatcag gaattgccgg tatttgaata cgcggcacgt caggtaaagc
    13621 aaacggtggt aggtattggt agcgccgaga aaagccaggt gcagcatatg gtccgcacct
    13681 tgctgaaact gcccgctaat ccacaagcgg atgccgctga tgcgttagct atcgcgatta
    13741 cccattgtca cgtcagccag aatgcaatgc agatgagcga atcgcggctg aacctggcga
    13801 gagggcgact gcgttaagtt ataccgcctc ggtcagttca ggctgaggcg tttcaactcc
    13861 ctccgcgtga atcaacacca aacccggatg atgccacaaa ctggctggcg tgacggtttt
    13921 acgctcccac ggaatagaac ctaagaacca gaatttccag aaagtgagtt ttgcattcgg
    13981 attgctattt aacagttgtc caaacgtctc tatttcaccc accggaatac atagcgcctc
    14041 tttataaata tcaaagacaa attttgaaca gaactggcgg gaagattcgt atttaaaacc
    14101 cgtgtggtaa agtttgcgta gccgggaggg aacctgttca acaattcgtt gtttctgttg
    14161 ttctgtcagc ccggcgtcta atcgctttat agcatagcgt tgattagcag agcgtttaat
    14221 aaaacgggat agcgtagtga tggttgagag gggaacacgg ctttctgcaa ccagaaagtc
    14281 ttcaccgtta tgaccgataa tgatcccgac gtgattactc cagcagtttg atgcagctga
    14341 tatttgacca aataaggcag cacctataca tgtaaagacg atatcaccaa tttcatattc
    14401 agcagggtaa ttaatattca cgttgtcatt ccattgaaat agatatgaat atacgtaata
    14461 gcataaaaaa tttattattt caggagtcgc aatgtatatt tttattacgc atttcttcac
    14521 tgaatatgta atattcaaat atttgcttcc aatataacct gtagaataaa ttatactgcg
    14581 ccatttttca gatcgttggg acgcctcgca agttttcttc atccttcgct ggatatctat
    14641 ccagcatttt tttatcatac agcattatct ttgattcatt acgcaggagc gtcatgtgat
    14701 aggcagactc agaggcatca tcattgaaaa acaacccccg ctggtgttaa ttgaagtggg
    14761 cggcgtaggc tatgaagtgc atatgccgat gacctgtttt tatgaactcc ctgaagcggg
    14821 tcaggaagcg atcgttttca cccactttgt ggtgcgtgaa gacgcgcaac tgctgtacgg
    14881 ttttaacaat aaacaagagc gcacattgtt caaagagttg atcaaaacca acggcgtcgg
    14941 cccgaagttg gcgctggcga tcctctccgg aatgtcagcg cagcagttcg ttaatgccgt
    15001 tgagcgtgaa gaagtggggg cactggtgaa actgccgggt attggcaaaa aaaccgccga
    15061 acgcttgatt gttgaaatga aagaccgatt taaaggtttg catggcgatc tctttacgcc
    15121 agccgccgac ctggtactca cgtcacctgc cagcccgacg accgacgatg ctgaacagga
    15181 agcggttgcc gcgctggtgg cgctgggcta taaaccacaa gaagcaagcc gcatggtgag
    15241 caaaatcgct cgccctgacg ccagcagtga aacattaatt cgcgaagccc tacgcgccgc
    15301 gttatgaggt aaaggatgat tgaagcagac cgtctgattt ctgccggtac cactttgccg
    15361 gaagatgtag cagatcgcgc cattcgccct aaattactgg aagagtatgt tggtcagccg
    15421 caggttcgtt cacagatgga gattttcatc aaagcagcga aactgcgcgg cgatgccctc
    15481 gatcatctgt tgatttttgg tcctccgggg ttgggtaaaa ctacgcttgc caacattgtc
    15541 gccaatgaaa tgggcgttaa tttacgcacg acttctggtc cggtgctgga aaaggcgggc
    15601 gatttggctg cgatgctcac taaccttgaa ccgcatgacg tgctgtttat tgatgagatc
    15661 caccgtttat cgccagttgt tgaagaagtg ctgtacccgg caatggaaga ctaccaactg
    15721 gatatcatga ttggtgaagg tccggcggca cgctccatta aaattgattt gccgccgttt
    15781 accctgattg gtgcaaccac gcgcgcaggt tcgctgacat caccgttgcg cgaccgtttt
    15841 ggtattgtgc aacgtctgga gttttatcag gtgccggatc tgcaatatat cgtcagtcgc
    15901 agcgcacgct ttatggggct tgagatgagt gatgacggcg cgctggaagt tgctcgtcga
    15961 gctcgcggta cgccgcgcat tgccaaccgt ctgctgcgtc gagtgcgtga tttcgccgaa
    16021 gtgaagcacg atggcaccat ctcggcagat atcgctgctc aggcgttgga tatgttgaat
    16081 gtcgatgctg aaggtttcga ttatatggac cgcaagttat tgctggcggt aatcgataag
    16141 ttctttggtg ggccggtagg tctggataac cttgcggcag ccattggcga agaacgtgaa
    16201 accattgagg atgtgctgga accttatttg attcagcaag gctttttgca gcgtacaccg
    16261 cgtgggcgta tggcgacgac gcgggcgtgg aatcactttg gcataacgcc gccagaaatg
    16321 ccgtaagtcg gattggtcgc tcgcgccgca tccgacactg gcagtgtgtc tgatgcgaca
    16381 ctgttcgcgt cttatcaggc cagccagaga tagcgaaccg taggtcggat aaggcgctcg
    16441 cgccgcatcc gacaaatgtg ttcagcgata gattagctgg cctgcttttt catcatactg
    16501 agaataaata acagtgccgc acatagcacc accgacggac ccgccggcgt atcgtaaaac
    16561 gcggaaaagg ttaaaccgcc agttactgcc accatcccca ccaaaacagc gacaccagcc
    16621 atctgttccg gcgtacgggc aaagcgacgc gcagtagctg caggaataat cagcagtgaa
    16681 gtaataatca acgcgccaac aaatttcatc gctacaccaa tcgtcaatgc cgtcaccagc
    16741 atcaacaaca atttcacgcg ctgtaatttc acaccatcaa caaacgccag atccgggcta
    16801 atcgtcatcg acagcaaatt gcgccattgc cagaacaaaa tagccaccac gatgaccacg
    16861 ccaatcgcaa tagcgatgag atcttctggc gtcactgcca gcaaatcacc gaacaggtaa
    16921 gccatcaaat caacacgaat gttagacatc agactaacga ccaccaggcc cagcgacagg
    16981 gcactgtgcg ccataatccc taataacgtg tcaatcgcca gctgtggacg cttctccagc
    17041 cataccagac cgcccgccag gagcagcgta acggcaatta ccgcatagaa tggattcacg
    17101 tccagcaaca aaccaaacgc gacgccaagt aatgaggcat gagccagcgt atcaccgaaa
    17161 taagacatac gacgccagac tacaaacgaa cccagcggac ccgcggcaca ggcgagcatg
    17221 atcccggcta accaaccggg aaataataat tcaatcatga gcgatcattt ccccgacgca
    17281 aaacaattcg tccctgtaaa tcgtgacgat gattatgatg atggcgatag atacccagtt
    17341 gttcagcacc acgaggacca aacatagaaa taaactccgg atgcagggaa acaacttccg
    17401 gtgtgccgga acaacaaatg tggtgattca gacacagcac ttcatcggtt tttgccatta
    17461 ccagatgcag atcgtgagaa accattaaaa cgccacaatc cagttcacga cgcagttggt
    17521 caataaggtc atataacgcc acctggccat ttacatccac gccttgagtg ggttcatcca
    17581 gcactaataa ttgcgggcga tttaacaatg ctcgcgctaa tagtacacgc tgcgtttcgc
    17641 caccagagag cttttgcatc ggtgcgttaa tcaggtgccc ggcctggaca cgtttcagtg
    17701 caggcaaaat atcttcttta tgtgtaccag ggcgtaagcg taaaaaacgg tttacggtca
    17761 gtggcaacgt ggtgtcgaga tacagcttct gcggtacata gccgatgcgc agttttccgt
    17821 tgcgcttgat aaccccttca tcgggtgtta ccagcccgag cactacccgt accagtgtcg
    17881 acttacctgc gccatttggc ccaagtaaag tcaaaatttt tccaggttta agttccagcg
    17941 acacatcaga gaggacgcgg cgttggccaa aagaaaccga gacattttcc agggaaacca
    18001 gacttgtcat gttaatttta gtcttgcagt agtcatgaaa tgttataata tcacacttct
    18061 catattcatt acgatgattg gtcgcattat gttacataaa aaaacgcttc ttttcgcagc
    18121 attatccgcc gctctctggg gaggtgcaac acaggccgca gatgccgccg ttgtcgcttc
    18181 gcttaaaccc gttgggttca tcgcttctgc cattgctgat ggtgtaacag aaacgcaggt
    18241 tttactgcct gacggcgctt cagaacatga ttattcactg cgtccatcgg atgtaaaacg
    18301 cttacagaac gcggacttag tcgtttgggt tggcccggag atggaagcgt ttatgcaaaa
    18361 accggtaagc aaattaccag aagcgaagca ggtaacgatt gcgcagcttg aagatgtgaa
    18421 accgctactg atgaaaagta ttcacggcga tgatgatgat cacgaccacg cggaaaaaag
    18481 tgacgaagat caccatcacg gcgatttcaa catgcatctt tggctttccc cagagatagc
    18541 gcgggctaca gcggttgcaa tccatggaaa attagtggaa cttatgccgc aaagtcgagc
    18601 caaacttgac gccaacctga aggattttga ggcacaatta gcctcaaccg aaacgcaggt
    18661 tggtaacgag ctcgcgccgc tcaaggggaa aggttatttc gtttttcacg atgcttacgg
    18721 ctatttcgaa aaacagtttg gactgacacc gcttggtcat tttaccgtta accctgagat
    18781 tcaacctggc gcgcagcgtt tacatgaaat aagaacacag ttggttgagc aaaaagcaac
    18841 ctgcgttttt gctgagccac agttcaggcc agcggtcgtt gagagcgtcg cacgagggac
    18901 atccgttcgt atgggaacgt tggatcccct ggggacgaat atcaaactgg gtaaaacaag
    18961 ctattcagaa ttcctgagtc aattagccaa ccagtatgcg agctgcctga aaggagatta
    19021 atgaggaagt gattacgtgc aacagatagc ccgctctgtc gccctggcgt ttaataattt
    19081 accgcgacca caccgcgtta tgttggggtc gctcaccgtt cttactctgg ccgtcgctgt
    19141 ctggcggccc tatgtttatc accgcgatgc cacgccaatt gtcaaaacca ttgagctgga
    19201 acagaacgaa attcgttcgc tcttacctga agccagtgag ccgattgatc aagctgcaca
    19261 agaagatgaa gccattcctc aggacgaact ggatgacaaa atcgccggtg aagcgggcgt
    19321 gcatgaatat gttgtttcca ctggcgatac gctaagcagc attctcaatc agtatggtat
    19381 tgatatgggt gatatcaccc aactggctgc ggccgacaaa gaattgcgta acctgaaaat
    19441 cggtcaacaa ctctcctgga cattaaccgc ggacggcgaa ctgcagcgcc tcacctggga
    19501 agtgtctcgt cgtgaaaccc gaacctatga ccgtactgcc gctaacggtt ttaaaatgac
    19561 cagcgaaatg cagcaaggag agtgggttaa caatctgctg aaaggtaccg tcggggggag
    19621 ctttgttgcc agcgccagaa acgccggttt aaccagcgcc gaagtgagcg cagtgattaa
    19681 agccatgcag tggcaaatgg atttccgcaa actgaaaaaa ggcgatgaat ttgcggtatt
    19741 aatgtctcga gaaatgcttg atggtaaacg tgagcaaagc cagctgctgg gcgtacgttt
    19801 gcgttcagaa ggtaaagatt attacgctat ccgcgctgaa gatggcaaat tctacgatcg
    19861 taacggtact ggtctggcga aaggattctt gcgattcccg acggcgaaac agttccgtat
    19921 ctcgtctaac tttaacccgc gtcgtactaa tccggtgacc ggtcgcgttg caccacacag
    19981 aggtgttgat ttcgccatgc cacaaggtac gccagtgctt tcagtgggtg acggtgaagt
    20041 ggtggttgcc aaacgcagtg gtgcagcagg ttattatgtg gctattcgtc atggtcgcag
    20101 ctacaccacg cgttatatgc acttgcgcaa gattctggtg aaaccgggac agaaggtgaa
    20161 acgtggcgac cgtatcgcgc tttccggtaa taccggacgt tcaaccgggc cgcatctgca
    20221 ctatgaagta tggataaacc agcaggccgt aaacccgctg acggcaaaac tgccgcgtac
    20281 cgaagggctg accggctccg atcgtcgcga attcctggcg caggccaaag agattgtgcc
    20341 gcagctacgg tttgattaat taacatccat tcgcagccgg tacgaagtca gtaccggctt
    20401 tttttatttg gtgcggggca agttgcgccg ctacactatc accagattga tttttgcctt
    20461 atccgaaact ggaaaagcat ggaaacgaaa aaaaataata gcgaatacat tcctgagttt
    20521 gataaatcct ttcgccaccc gcgctactgg ggagcatggc tgggcgtagc agcgatggcg
    20581 ggtatcgctt taacgccgcc aaagttccgt gatcccattc tggcacggct gggacgtttt
    20641 gccggacgac tgggaaaaag ctcacgccgt cgtgcgttaa tcaatctgtc gctctgcttt
    20701 ccagaacgta gtgaagctga acgcgaagcg attgttgatg agatgtttgc caccgcgccg
    20761 caagcgatgg caatgatggc tgagttggca atacgcgggc cggagaaaat tcagccgcgc
    20821 gttgactggc aagggctgga gatcatcgaa gagatgcggc gtaataacga gaaagttatc
    20881 tttctggtgc cgcacggttg ggccgtcgat attcctgcca tgctgatggc ctcgcaaggg
    20941 cagaaaatgg cagcgatgtt ccataatcag ggcaacccgg tttttgatta tgtctggaac
    21001 acggtgcgtc gtcgttttgg cggtcgtctg catgcgagaa atgatggtat taaaccattc
    21061 atccagtcgg tacgtcaggg ctactgggga tattatttac ccgatcagga tcatggccct
    21121 gagcacagcg aatttgttga tttctttgcc acctataaag cgacgttgcc cgcgattggt
    21181 cgtttgatga aagtgtgccg tgcgcgcgtt gttccgctgt ttccgattta tgatggcaag
    21241 acgcaccgtc tgacgattca ggtgcgccca ccgatggatg atctgttaga ggcggatgat
    21301 catacgattg cgcggcggat gaatgaagaa gtcgagattt ttgttggtcc gcgaccagaa
    21361 caatacacct ggatactaaa attgctgaaa actcgcaaac cgggcgaaat ccagccgtat
    21421 aagcgcaaag atctttatcc catcaaataa aaaaagcctc tcgcgaggag aggccttcgc
    21481 ctgatgataa gttcaagttt gcttcagaat attcgaaatc tgttgaacta tcattgaact
    21541 gtaggccgga tgtggcgttt tcgccgcatc cggcaacgta cttactctac cgttaaaata
    21601 cgcgtggtat tagtagaacc cacggtactc atcacgtcgc cctgggtgac aatcactagg
    21661 tcaccagaca tcaagtaacc tttatcgcgc agcagattaa ccgcttcgct ggcagctgct
    21721 acgccgtcat tagcgctatc aaagtgcacc ggcgtaacgc cacgatagag agcagtcagg
    21781 ttcagcgtac gttcatggcg cgacatggcg aaaattggca gaccagagct gatacgggag
    21841 gtcatcagcg cggtacgacc cgattcggtc atggtaatga tcgccgtaac gcccttcagg
    21901 tggttagccg cgtacattgc cgacatggca atagcttctt ccacattgtc gaactgaacg
    21961 tccagacggt gtttagaaac gttgatgctc gggatttttt ccgcacccag gcaaacgcgt
    22021 gccatggctg caacggtttc tgacggatac tgcccagcgg cagtttctgc agacagcatc
    22081 acagcgtcag taccatccag aacggcgttt gctacgtcca tgacttctgc acgcgtcggc
    22141 atcgggttag taatcattga ctccatcatc tgggtcgccg tgattaccgc tcggtttagc
    22201 tgacgtgcac ggcggatcaa cgctttctga atgccgacca gttccgggtc gccaatttcc
    22261 acaccgaggt cgccacgtgc aaccattacc acgtcagagg cgaggatgat gtcatccatt
    22321 gcatcctggc tgcaaacggc ttccgcacgt tcaaccttgg caacaatttt cgcatcacat
    22381 cctgcatcgc gtgccaggcg acgggcatag ttcagatctt cgccacagcg tgggaaggag
    22441 acagccaggt aatctacgcc aatcaacgcc gcagtcttaa tgtctgcttt gtctttttcg
    22501 gtcagcgctt cagccgacaa accgccgcca agtttgttga tacctttatt gttggagagg
    22561 ggaccaccga cggtgacttc ggtgaacact ttcatgccct gaacttccag tacttttaac
    22621 tggacgcgac catcgtccag cagcaggatg tcaccaggca cgacgtcagc aggcaggcct
    22681 ttgtagtcga taccgacttt ttctttgtcg ccttcacctt tacccaggtt ggcgtcgagc
    22741 aggaatttat ccccaatatt gaggaaaact ttgccttctt taaaggtgga tacacggatt
    22801 ttgggcccct ggaggtcacc cagaatagcc acatgacgcc ccagtcttgc ggcaatctca
    22861 cgaactttat ccgcgcgcat tttgtgatct tcaggcgagc cgtgagaaaa gttcatacgt
    22921 acaacgttgg cacccgccgc gataactttt tcaagattat tatcgcgatc tgttgctggg
    22981 cctaacgtgg taacgatttt tgttctgcga agccttctgg acatgtaata ctccgttgac
    23041 tgaaacaacc aggtgttgct tgaacatgaa atccgaatga aataacgccg cgatggcgat
    23101 aaattaatct cattcggatg tcgtataaga ttaggacagt gacagtcgtt tttagcgatc
    23161 gtcacttaaa ttaagtaact gcttatcaaa acgcgattcc ttcagcgctt ccttgacccg
    23221 cttcaagtta tctctgaatt ttgcaccgcg tcgcaaagta aatcctgtcg ccagcacatc
    23281 tatcacggtc agctgtgcaa gtcgagaaac catgggcata taaatgtcag tatcttccgg
    23341 tacgtcgagg gtaattgcca gcgttgcttc ccgggcgagc ggggtacccg cagaggtgag
    23401 ggcaatcacc attgcgtcgt tttcgcgagc cagctgcgcc agctcgacca gattttttgt
    23461 tcttccagtg tgagaaatca gcaccaccac gtctccgtcg ctacaattca tacaactcat
    23521 gcgttgcagc acgatatcat cggagtacac caccggaaca ttaaaacgaa agaacttatt
    23581 catcgcatcg tgggcaacgg cggctgaaga gcctaatccg aaaaaggcga ttttttttgc
    23641 ctgagtgagc aagtcgacgg cgcggttgat ggcagattta tccagtgaat gacggacatg
    23701 atcaagcgtt gccattgcgg actcaaatat tttccctgtg tatgattcaa cgctgtcatc
    23761 ttcattgaca ttgcgattaa catagggagt gccattcgcc agactttgtg ccagatgaag
    23821 tttaaaatca ggaaaaccgc gcgtgtccat gctgcgacag aaacgattca ccgtcggttc
    23881 gctaacattg gcttccagcg ccagagcagc aatactcgaa tggatcgcgt tatcgggcga
    23941 agccagaatg acctcggcaa ccttgcgctc tgatttgctc aaatgttcca gctgagactg
    24001 gattttttcc agcatattca tgatgtaaag agactcacgg gtaatgacga tttccgcact
    24061 gaaagaaatc gaaatgcagt tttgtcagat attacgcctg tgtgctgtgt taatgacaaa
    24121 agcagataaa aaagttgtta ttttttttca taacatgatc agtgtcagat ttttacccaa
    24181 tggaaaacga tgattttttt atcagttttg ccgcactttg cgcgctttta ccgtaatcgc
    24241 acgggtggat aagcgtttac agttttcgca agctcgtaaa agcagtacag tgcaccgtaa
    24301 gaaaattaca agtataccct ggcttaagta ccgggttagt taacttaagg agaatgacat
    24361 ggcggtaacg caaacagccc aggcctgtga cctggtcatt ttcggcgcga aaggcgacct
    24421 tgcgcgtcgt aaattgctgc cttccctgta tcaactggaa aaagccggtc agctcaaccc
    24481 ggacacccgg attatcggcg tagggcgtgc tgactgggat aaagcggctt ataccaaagt
    24541 tgtccgtgag gcgctcgaaa ctttcatgaa agaaactatt gatgaaggtt tatgggacac
    24601 cctgagtgca cgtctggatt tttgtaatct cgatgtcaat gacactgctg cattcagccg
    24661 tctcggcgcg atgctggatc aaaaaaatcg tatcaccatt aactactttg ccatgccgcc
    24721 cagcactttt ggcgcaattt gcaaagggct tggtgaggca aaactgaatg ctaaaccggc
    24781 acgcgtagtt atggagaaac cgctggggac gtcgctggcg acctcgcagg aaatcaatga
    24841 tcaggttggc gaatacttcg aggagtgcca ggtttaccgt atcgaccact atcttggtaa
    24901 agaaacggtg ctgaacctgt tggcgctgcg ttttgctaac tccctgtttg tgaataactg
    24961 ggacaatcgc accattgatc atgttgagat taccgtggca gaagaagtgg ggatcgaagg
    25021 gcgctggggc tattttgata aagccggtca gatgcgcgat atgatccaaa accacctgct
    25081 gcaaattctc tgcatgattg cgatgtctcc gccgtctgac ctgagcgcag acagcatccg
    25141 cgatgaaaaa gtgaaagtac tgaagtctct gcgccgcatc gaccgctcca acgtacgcga
    25201 aaaaaccgta cgcgggcaat atactgcggg cttcgcccag ggcaaaaaag tgccgggata
    25261 tctggaagaa gagggcgcga acaagagcag caatacagaa accttcgtgg cgatccgcgt
    25321 cgacattgat aactggcgct gggccggtgt gccattctac ctgcgtactg gtaaacgtct
    25381 gccgaccaaa tgttctgaag tcgtggtcta tttcaaaaca cctgaactga atctgtttaa
    25441 agagtcgtgg caggatctgc cgcagaataa actgactatc cgtctgcaac ctgatgaagg
    25501 cgtggatatc caggtactga ataaagttcc tggccttgac cacaaacata acctgcaaat
    25561 caccaagctg gatctgagct attcagaaac ctttaatcag acgcatctgg cggatgccta
    25621 tgaacgtttg ctgctggaaa ccatgcgtgg tattcaggca ctgtttgttc gtcgcgacga
    25681 agtggaagaa gcctggaaat gggtagactc cattactgag gcgtgggcga tggacaaaga
    25741 tgcgccgaaa ccgtatcagg ccggaacctg gggacccgtt gcctcggtgg cgatgattac
    25801 ccgtgatggt cgttcctgga atgagtttga gtaatatctg cgcttatcct ttatggttat
    25861 tttaccggta acatgatctt gcacagattg tagaacaatt tttacacttt caggcctcgt
    25921 gcggattcac ccacgaggct ttttttatta cactgactga aacgtttttg ccctatgagc
    25981 tccggttaca ggcgtttcag tcataaatcc tctgaatgaa acgcgttgtg aatcatcctg
    26041 ctctgacaac tcaatttcag gagcctttat gaatccacaa ttgttacgcg taacaaatcg
    26101 aatcattgaa cgttcgcgcg agactcgctc tgcttatctc gcccggatag aacaagcgaa
    26161 aacgtcgact gttcatcgtt cgcagttggc atgcggtaac ctggcacacg gtttcgctgc
    26221 ctgccagcca gaagacaaag cctctttgaa aagcatgttg cgtaacaata tcgccatcat
    26281 cacctcctat aacgacatgc tctccgctca ccagccttat gaacactatc cagaaatcat
    26341 tcgtaaagcc ctgcatgaag cgaatgcggt tggtcaggtt gcgggcggtg ttccggcgat
    26401 gtgtgatggt gtcacccagg ggcaggatgg aatggaattg tcgctgctaa gccgcgaagt
    26461 gatagcgatg tctgcggcgg tcgggctgtc ccataacatg tttgatggtg ctctgttcct
    26521 cggtgtgtgc gacaagattg tcccgggtct gacgatggca gccctgtcgt ttggtcattt
    26581 gcctgcggtg tttgtgccgt ctggaccgat ggcaagcggt ttgccaaata aagaaaaagt
    26641 gcgtattcgc cagctttatg ccgaaggtaa agtggaccgc atggcactac tggagtcaga
    26701 agccgcgtct tatcacgcgc cgggaacatg tactttctac ggtactgcca acaccaacca
    26761 gatggtggtg gagtttatgg ggatgcagtt gccaggctct tcttttgttc atccggattc
    26821 tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag gttacacgca tgaccggtaa
    26881 tggtaatgaa tggatgccga tcggtaagat gatcgatgag aaagtggtgg tgaacggtat
    26941 cgttgcactg ctggcgaccg gtggttccac taaccacacc atgcacctgg tggcgatggc
    27001 gcgcgcggcc ggtattcaga ttaactggga tgacttctct gacctttctg atgttgtacc
    27061 gctgatggca cgtctctacc cgaacggtcc ggccgatatt aaccacttcc aggcggcagg
    27121 tggcgtaccg gttctggtgc gtgaactgct caaagcaggc ctgctgcatg aagatgtcaa
    27181 tacggtggca ggctttggtc tgtctcgtta tacccttgaa ccatggctga ataatggtga
    27241 actggactgg cgggaagggg cggaaaaatc actcgacagc aatgtgatcg cttccttcga
    27301 acaacctttc tctcatcatg gtgggacaaa agtgttaagc ggtaacctgg gccgtgcggt
    27361 tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt gaagcgccag cggttgtttt
    27421 tgaaagccag catgacgtta tgccggcctt tgaagcgggt ttgctggacc gcgattgtgt
    27481 cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg ccagaattac ataaactcat
    27541 gccgccactt ggtgtattat tggaccggtg tttcaaaatt gcgttagtta ccgatggacg
    27601 actctccggc gcttcaggta aagtgccgtc agctatccac gtaacaccag aagcctacga
    27661 tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt cgtgtgaatg gacagacagg
    27721 cgaactgacg ctgctggtag acgaagcgga actggctgct cgcgaaccgc acattcctga
    27781 cctgagcgcg tcacgcgtgg ggacaggacg tgaattattc agcgccttgc gtgaaaaact
    27841 gtccggtgcc gaacagggcg caacctgtat cactttttaa gacgacaaat ttgtaatcag
    27901 gcgagagaaa actctgatga aaaactggaa aacaagtgca gaatcaatcc tgaccaccgg
    27961 cccggttgta ccggttatcg tggtaaaaaa actggaacac gcagtgccga tggcaaaagc
    28021 gttggttgct ggtggggtgc gcgttctgga agtgactctg cgtaccgagt gtgcagttga
    28081 cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt gtgggtgccg gtacggtact
    28141 gaatccacag cagctggcag aagtcactga agcgggtgca cagttcgcaa ttagcccggg
    28201 tctgaccgag ccgctgctga aagctgctac cgaagggact attcctctaa ttccggggat
    28261 cagcactgtt tccgaactga tgctgggtat ggactacggt ttgaaagagt tcaaattctt
    28321 cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg atcgcgggtc cgttctccca
    28381 ggtccgtttc tgcccgacgg gtggtatttc tccggctaac taccgtgact acctggcgct
    28441 gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg gcagatgcgc tggaagcggg
    28501 cgattacgac cgcattacta agctggcgcg tgaagctgta gaaggcgcta agctgtaaaa
    28561 gtcaaaacgc ccgatctacg atcgggcatt tttgtagcgt ttttttgccg ggtttaaccc
    28621 tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa tggcgtcaac aacactctca
    28681 gcggtcgcca gcgcaacacc cagacgacgg ctgccatcaa tttccggctt accaaataaa
    28741 cgaatctgca aatctgcgcc tacggcattc tgcacattat caaacgtgac attctgactg
    28801 gtcagttgtg gcagaataac ggcagaagct gcaggaccat actgacggat cccgccaaca
    28861 ggaagtccga ggaaggcacg tacatgcagg gcaaactctg agagatcttg agaaattaac
    28921 gtcaccatcc cggtatcatg tggacgaggg gagacctcac tgaaaatcac ctcatcacca
    28981 cagacaaaca gctcgacacc aaacagacca taaccgccca gtgccagcac cactttacgg
    29041 gcaatctcct gcgcacgttc aagggcaagc gggctcattt gctgtggttg ccaggattca
    29101 cggtagtcgc catcttcctg gcgatgacct actggtgcac agaaatggac gccatccacc
    29161 gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa cgacgccttc aacaattacg
    29221 cggcccgctc cggcgcgacc gccttgctga gcgtattccc atgcctgagc aagttgctct
    29281 gcagagcgaa taaacgtctg ccccttgccg gaagagctca tcaccggttt tacaatgcag
    29341 ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt cgctatcggc aaaacgataa
    29401 gtggaagtgg acagctgcag ctcttctgcc gccaggcgac ggataccctc gcgattcatc
    29461 gttaatttcg ttgcgcgagc gcaggggaca acattcagtc cttcctcttc aagttggatc
    29521 agcatatcgg tggcaatagc ttcgatctcc ggcacgatat aatgtggttt ttccagttca
    29581 accacacggc gtaatgcatc accatcaagc atattaatga catgggagcg atgcgcgaca
    29641 tgcatggctg gtgcgtcggc atagcgatcg acggcaatca cctctacgcc gagacgctga
    29701 cactcgattg ccacttcttt acccagttca ccggagccta ataacatcac gcgagttgct
    29761 gccggacgca gcgcagtgcc taataacgtc ataacgaata tcccctgatt aattccgcgc
    29821 gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg gtgttgattt ctcaaccgaa
    29881 aagaaatata ctgtataaaa tcacagttat tatgagaggc tgcaaatggc ggttgaagtt
    29941 aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt ttaccagcaa aaaggaagcc
    30001 gatgcttatg acaaaatgct tgatacggcg gatctgcttg acacctggct gacaaattct
    30061 ccagtgcaaa tggaagacga gcaacgtgaa gctctttcgc tatggctggc agaacaaaaa
    30121 gatgtgctga gcaccattct gaaaaccggc aaattaccgt ccccacaggt cgtcggtgct
    30181 gaatccgaag aggaagatgc ctcacacgcg gcctgaagca acttgcgctc ctgacaattt
    30241 gtaccatgct ttctcctgaa tcattgtgct atggagaaaa acatgaaaaa aagaggggcg
    30301 tttttagggc tgttgttggt ttctgcctgc gcatcagttt tcgctgccaa taatgaaacc
    30361 agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg ctgccggaat tgccgcgagc
    30421 gtaaaacgtg attatcaaca aaatcgcgtg gcgcgttggg cagatgatca aaaaattgtc
    30481 ggtcaggccg atcccgtggc ttgggtcagt ttgcaggaca ttcagggtaa agatgataaa
    30541 tggtcagtac cgctaaccgt acgtggtaaa agtgccgata ttcattacca ggtcagcgtg
    30601 gactgcaaag cgggaatggc ggaatatcag cggcgttaat ggcttctgat aattcgattg
    30661 accgttggcg gatgcggcgc gagtgccttg tccgcccaca acacaccaca accgtaggcc
    30721 tgagaagacg caacagcgta gcatcggaca atcagcatca tacagtgcca ctcaaaacgt
    30781 caaactactt tcacctaaaa tacccttatc acctgatata cagtaaacac cttacctggc
    30841 gcgaatttct ttgaataacg gcctaccgcc ccgcttattt gcgcatcttt tgtctcgata
    30901 tacattttct gacatcctca tccgctatgc tacaggcact aaaaatggtt attgaggagc
    30961 aaatatggct aactggttaa atcaactgca atccctgctt gggcaaagca gttcttctac
    31021 ctcctcgtct gcggatcagg gattgggcaa actgttagtg ccaggcgcat taggcggact
    31081 ggctgggctg ctggtcgcaa ataaatcagc acgtaaactt cttacgaaat atggcaccaa
    31141 cgcgttactg gttggcggcg gagcggtagc gggtacggtg ctgtggaata aatacaaaga
    31201 taaaattcgc gcggcgcatc aggacgaacc gcagtttggc gcgcaaagta cgccgctgga
    31261 tgagcgtaca gaacgtttga tccttgcgct ggtctttgcc gctaaaagtg atggtcatat
    31321 tgatgccaaa gaacgtgcgg caatcgacca gcaattgcgt gaagccggcg tggaagagca
    31381 ggggcgtgta ctcattgagc aggcaatcga acaaccgctg gatccacaac gcctggctac
    31441 cggggtccgc aatgaagaag aggcgctgga aatctatttc ctgagttgcg cggctattga
    31501 tattgaccat tttatggaac gcagttacct gaatgcactg ggtgacgcgc tgaaaatccc
    31561 ccaggatgtt cgtgacggca ttgaacgcga tctcgaacag caaaaacgca ctttggcgga
    31621 ataattccgc tctctggata gcctgaagtt gactatttcg ggctatccag aatcgcacag
    31681 ccatttgaac aggctgattt tctctgtctg cataacattc gatatccgcg ccattacctg
    31741 tcatcatcta agcaatgact cccctgtttc gcttgcatcc ccggtgagtt ttgccaccct
    31801 tataagatgt ttcaaccaga aagaacaata acatgctacc aaaagccgcc cgcattcccc
    31861 acgccatgac gcttcatggc gatacgcgca tcgataatta ctactggctg cgggacgata
    31921 cgcgttctca gccagaagtc ctggactacc tgcaacaaga aaatagttac ggtcatcggg
    31981 tgatggcctc acaacaagcc ttgcaggatc gcatcttaaa ggaaatcatc gaccgcattc
    32041 cgcaacgaga agtttctgcg ccctacatca aaaatggcta ccgctatcgg catatttatg
    32101 aaccaggctg tgaatatgct atctaccagc gtcaatcggc attcagtgaa gagtgggatg
    32161 agtgggaaac attgctcgat gccaataagc gcgcagctca tagtgagttt tattcgatgg
    32221 gcggaatggc gattacgccc gataacacca ttatggcgct ggcagaagat tttctttccc
    32281 gacgccagta cggcattcgt tttcgtaatc tggaaactgg taactggtac ccggaactgc
    32341 tggataacgt tgaacccagc tttgtctggg caaatgactc ctggactttc tactatgttc
    32401 gcaagcatcc ggtgacgctg ctgccttatc aggtctggcg tcacgccatc ggtacgccag
    32461 catcgcaaga taaactgatc tacgaagaaa aagacgatac ctattacgtc agcctgcata
    32521 aaacgacctc gaagcactat gtagtcattc atttggccag cgccaccacc agtgaagttc
    32581 gcctgctgga cgcggaaatg gccgatgccg agccgtttgt ttttctgccg cgccgcaaag
    32641 atcacgaata cagccttgat cactaccagc atcgttttta tctgcgttcc aaccgccacg
    32701 gcaaaaactt tggcttatac cgtacccgta tgcgtgatga gcaacagtgg gaagagttaa
    32761 ttccgccacg cgaaaacatc atgctggaag ggtttacgct gtttaccgac tggctggtgg
    32821 ttgaagagcg tcagcgcggg ttaaccagtt tgcgccaaat taaccgcaag acccgggaag
    32881 tcattggtat tgcctttgat gatccggcct atgtgacctg gattgcctac aatccagaat
    32941 ctgaaaccgc gcgattgcgt tatggttatt cttccatgac cacaccagac actttgtttg
    33001 aactggatat ggataccggt gagcgtcgtg tattaaaaca aacggaagtt cctggttttg
    33061 atgcggcgaa ttaccgcagt gaacacctgt ggatagtcgc ccgtgatggc gtcgaagttc
    33121 cggtttcgtt gggctaccat cgcaaacatt ttcgcaaagg acacaacccg ttgctggtgt
    33181 atggctatgg ttcttacggc gcaagtattg atgccgattt cagttttagc cgcttgagtt
    33241 tgttagatcg tggctttgtc tacgccattg tccatgttcg cggcggtggt gagctggggc
    33301 aacaatggta cgaagacgga aaatttctga aaaagaaaaa tacgtttaat gattatcttg
    33361 atgcctgcga tgcattgtta aaactgggct atggctctcc ttcgctttgt tatgcgatgg
    33421 gcgggagtgc ggggggcatg ttgatgggcg ttgcaattaa tcaacgcccg gaattattcc
    33481 acggcgttat cgcccaggta ccgtttgttg atgttgtaac aacgatgctt gatgaatcaa
    33541 ttcctcttac cactggtgag tttgaagagt gggggaatcc gcaggatccg caatattacg
    33601 agtacatgaa aagctacagc ccatatgaca acgtcaccgc acaggcttat ccgcatttac
    33661 tggtaacgac cggtttgcac gattctcagg tgcaatattg ggaaccggca aaatgggtcg
    33721 ctaaattgcg cgagctgaaa accgatgacc atcttttatt gctctgtacc gacatggact
    33781 caggccatgg cggtaaatct ggtcgcttta aatcgtacga aggcgtagcg atggaatatg
    33841 cttttctggt cgcgctggcg cagggaacat tacccgctac gcctgcggac taagtatttt
    33901 ccagataatg tttcagtgtt aaacgcagct ccgggctcat gctgtccagg ttattaaata
    33961 accagcgcag atagcccgga tcgcgttcgg caacgtcgga aaccgctttg ccacggtatt
    34021 tgccaaaggt gaaggtcgtc atcaacgacg gacgtccggt gatatcggcc atctgttctg
    34081 ccgtccagcc ggaggtgttc ataatatcga taagcaacgc ggcggtgata taacagtcat
    34141 acaacgcgcg gtgatgatgc aggcccggcg gcgtctgtac attgagcttg cgtgttttat
    34201 ataacgccat attgctgtac ttgatcccag gccacaaacg acgggccagt ttcatagtgc
    34261 aaatccactc accgggcatc tcaggcagta cgcggcggtc aaagctggcg ttatgcgcga
    34321 cataccattc actaccgtaa tagtgtggga tcacatcttc aatccacggt ttatcggcga
    34381 ccatggcttc ggtgatgcga tgaatcgcca tcgcttgtgg actaatagga cgatcggggc
    34441 gcaccaggtg gctcatgggg ttgacgattt ttccgtcaat gacatcaaca gaggcaatct
    34501 caacgatccc tccctgcaaa ccgcaggttt ctgtatcgat aatgcgcaac atggcatgct
    34561 ccaggccgaa aaagcctagc gtaatggaat gatatcgcct tgccaaccct gtgatgaacg
    34621 ctgaccgact aacaataatg aaccacgatc ggcgcgaacg atgagacgac catattcatc
    34681 ccatagtgca ctgttaccac gggcatttgc catcagcacg gcaatcgagt actgatgtga
    34741 aaaaaattgt aagcgacggg ccgacgccag caggtcgggt tctcccagac attgccctgt
    34801 agtaaacagt gaacaggtcg ggtccatatc cataccttcc ggttgttcat cgaccaccgt
    34861 gatggttctg gaacgacgac ccagacatgc accatgactc tgatggtaga tcccaggcgt
    34921 ttttcgccac ggtgcgaaca ccgcaattcc acgaataaag cgatcgttat attcaacggg
    34981 aaggccggca atgatggtca ttcgccaggt cgttgcggca taacaaagcg ggtctaacag
    35041 tgagagatcg ggtggggcag gaagggcacg tcgcgaataa tcacacccca gtaaagaaag
    35101 cgatggaaaa actaacagct gacattgttg cctggcggcg gccctcacaa actccagatg
    35161 atgagctacc tgttcggtga gtgaggtttt acgaggctca tattgcgctg ttgcaacttt
    35221 ccagaacgac ataatgactt ccttttcaaa atgactgcaa attatgggtg cggtttagaa
    35281 aatactcata atttagcagg cgttatgtaa gaaagtgtaa ctctgtataa gttttattta
    35341 agtttgggct cgtaaggtaa acgtgacaga ttgaccgaag ccaaacggtg ggcaataagc
    35401 cgctcgcgaa accagctgcg caaatgttca ggctgttcac gttcaaccgc ttcagcgatc
    35461 accggcatat tgtagcgttc tttaaatgcc accccggccg ccgccaaatc gacattcact
    35521 ttatccattt ctgtttgatc cagtttagcc agattcttca gcattcttaa tctcctcatc
    35581 attcgcgggc aaaagttact aagagtgagc caggatggca agccggaatt gatgatcctc
    35641 gactcagaca ttagacaagg ttatgctctg taaaaaagtt attacaaaaa ggaatagata
    35701 atatgacttc aactgcacgc tcccttcgct acgcgctggc aatcctgacc acttcgttgg
    35761 tgacaccttc cgtctgggca catgcgcatt taacgcatca gtatcctgcg gcaaacgcgc
    35821 aagtgacagc tgcaccgcag gcaatcactt taaacttctc ggaaggtgtt gaaacaggat
    35881 tcagtggtgc aaaaatcacg gggccaaaaa acgaaaatat taaaacatta ccggcgaagc
    35941 gaaatgagca ggaccaaaag caactgattg ttccactggc cgattcgctg aaacccggaa
    36001 cctacatcgt tgactggcat gttgtttcgg tggacgggca taaaacgaaa ggacactaca
    36061 cctttagcgt gaaataaatc atgctggcgt ttacctggat cgcgctgcga tttatccatt
    36121 tcacctcgct gatgctggtc tttggcttcg caatgtacgg tgcctggctg gcccccttaa
    36181 cgattcgtcg cttgctggcg aagcgttttc tgcgtttaca acagcacgct gccgtctgga
    36241 gtttgatcag tgccactgca atgctcgccg ttcagggcgg gttgatggga acgggatggg
    36301 cagatgtatt ttcgccaaac atttggcagg ctgttttgca aacgcagttt ggtggcgtct
    36361 ggttatggca aattgttctc gccctcgtaa cgttgatcgt cgcccttatg caaccgcgga
    36421 atatgccacg tttgctgttt atgctcacta ccgcgcaatt tattctgctg gcgggaatcg
    36481 ggcatgcgac actgaatgaa ggggtaacgg cgaaaatcca tcagactaat cacgcgattc
    36541 acctgatttg tgcggcggcg tggtttgggg gattactgcc ggtgctctgg tgtatgcaac
    36601 tcatcaaagg ccgttggcga caccaggcca ttcaggcgct gatgcgtttt tcctggtgcg
    36661 ggcattttgc ggtgatcggc gtactggcaa gcggcgtgct taatgcattg ttgattaccg
    36721 gatttccccc cacgcttacg acttactggg gccaactctt attgctcaaa gccatcctgg
    36781 tgatgattat ggtggtcatt gcgctggcta atcgttatgt tcttgtaccg cgcatgaggc
    36841 aagatgaaga tcgtgcggca ccctggtttg agtggatgac taaactcgaa tgggcgatag
    36901 gtgcggtagt gctggtgatc atcagcctgc ttgcgactct cgaacctttt tgatggatta
    36961 cgaaaatgat gaaaaaaagt attctgacgt ttctgttact caccagttct gcagcggcgc
    37021 tggctgcacc gcaggtgatt accgtcagtc gttttgaagt gggtaaagac aaatgggcgt
    37081 ttaatcgcga agaggtgatg ctgacttgca gaccgggtaa tgctttgtat gtcattaacc
    37141 caagtaccct cgtgcagtat cctttaaacg atatcgcaca aaaggaagtt gccagtggga
    37201 agactaaagc ccaacccatt tcggtgattc agattgatga tcctaacaat cccggcgaaa
    37261 aaatgagtct ggcaccgttt atagaacgag ctgaaaaact ctgttaatta cctaaaatag
    37321 ccttttgatt tccaataaaa aaaccgcctc agttctttca ccagaacggg cggtttttaa
    37381 catttcagct gatgaccacc acgcttttta ttgaccattt tgcacgcaaa ctggaaaacc
    37441 tggcgtcgtc atctattctt aaagggcaag gcaactaagc ctgcattaat gccaactttt
    37501 agcgcacggc tctctcccaa gagccatttc cctggaccga atacaggaat cgtgttcggt
    37561 ctctttttat ctgttaaaag ccagaagcat ttccttcgct gactttatag tcaaccataa
    37621 cacacactct actgtctgag tccagcgttt tttaacattc ttgttaagat tatgtgatct
    37681 ttagcgcggg aggaaaatat tgatgaaaca gcctgcgccc gtttatcaga gaattgcggg
    37741 tcatcaatgg cgacatatct ggctttctgg cgatatacac ggttgtcttg agcagttgcg
    37801 ccgcaaatta tggcattgtc gttttgatcc gtggcgagat ttacttatct cagtaggaga
    37861 cgttatcgat cgtgggccgc aaagtttacg ttgtctgcag ttactggaac aacattgggt
    37921 ttgtgcggta agaggcaatc atgaacagat ggcgatggat gcgctggcat cccagcagat
    37981 gtctttgtgg ttgatgaatg gcggcgactg gtttattgcg ctggcagata atcaacagaa
    38041 acaagcgaaa acggcgctgg aaaaatgtca gcatttgccc tttattcttg aagtacacag
    38101 tcgtaccggc aaacatgtta ttgctcatgc cgattatcca gatgatgttt atgaatggca
    38161 aaaggacgtt gatttgcatc aggtcttgtg gagccgctcg cgattaggtg aacgccaaaa
    38221 agggcaggga attacaggtg ctgatcattt ctggtttggt catacaccgt tgcgacatcg
    38281 cgtggatatt ggcaacctgc attatattga taccggtgct gtctttgggg gcgaactgac
    38341 tcttgtgcaa ttgcaataat taaaaatcac catactcctg tgctggtcgc cagaaaccat
    38401 ctataaaatc ctcaatcgga aaacaaccgc catggcggat ccgttgatcg ctcatagaat
    38461 aaagacactg ctgttccgtg ttgtagacat ccacaacaat atcttcacaa ccgccatcca
    38521 ggtagcaaac aaaaagtacc agcgcgaaca tttcatcccc gaagtgtggt gccgtaccgt
    38581 taagtttagg agagatttta caacggggga ataaccagga caaataaccc gccatgccga
    38641 cgggttcttt ttggatcagg caagacgcat aatccaggca tcagattgtt gatcgtaacg
    38701 ggtacggtaa aggacagagt gttcgccatt tagcagggca gggatactgg tttcagcatc
    38761 ccaggcatcc agttgcatac ataaatcggg gtcagattta caaggaatgg ttaacgttcg
    38821 atcacctggc gattcaccgt gtaattcgcc gtcgtcgatt tcaaaagcgc ctatgcgcac
    38881 actggttttc atcttcgtgc tctccattta gctgccggtt gtggtacgac cagtccgatc
    38941 gcaccatttt tcagcctagt tgaggcagcc gaatgtgcca gtcaaaaagt gcgctttttt
    39001 tgtgcgctgt caggcgttac cggtaaaaag tttcccatcg cgcaccagtt cacgcggata
    39061 gctgtttttc aatcgcgaac caatccgttt ggctaaacca atcggttgat gctggaaagt
    39121 aaccaacacg tcatccgcca ctggcgcggc ttgcgggtaa acatcgcgcc cgcgatacca
    39181 ctcctccgct tcctgcggtg tcagttcaaa agcgttcaca ttgtcggggg tggcaagggc
    39241 aataactgct tcatgctgcc agcgataacc tttgttgtgc gtttcggcaa gtttaatccc
    39301 caagcgagaa aatcggactt taccgatcag ggcttcaatg cccaccggga acaaccacag
    39361 ttctttgtcg cgttgccaga gtcgcaggtt tccatcccag tttaagccaa cacttgcagc
    39421 cgcctgacga atttgtccgg cttcgcgatc tttcaccggg ctgaacggga aattgcccac
    39481 tttgtatttg ggggcgggta aggcggggat cgcctgagtt ttacgcagac gagcaacgaa
    39541 gaagccttcg cagtcgtaaa tttgtgggaa aacatgcaaa aagccttctt cggtcagcgc
    39601 tttgtttgca ccagggaaga gatcgccaag tggtaaaaac tctactgcgt cggggtaagt
    39661 ctctttcagc cacaggcaaa cggcttcgtt ttcttcctgg tttaaggtac aggtcgagta
    39721 aaccagcgta ccaccaggac gtaatgcatg aaaggcgctg tcgataagct cccgttgtgt
    39781 agctgcgatt tcctgattgc tttctggtga ccagtttttt agcgcatcgg gatctttacg
    39841 caccacgcct tcgccagagc agggagcgtc cagcaaaatg gcatcgaaca tttctggcac
    39901 tgccgcacca aacacgcggc catcaaaatg tgtgagcgca acattactga tgccacagcg
    39961 gctgatattg gcatgtaaca cttttacccg actggcggaa aactcattgg caaggattgc
    40021 cccttcgtta ttcatccgcg cggcaatttg cgtcgttttg gagcctggcg cagctgcgac
    40081 atccatcacc cgctgtggtg cattatcgtc agcaaacaag gcggcaacgg gcaacattga
    40141 actggcttcc tgaatataaa acaggccgct taaatgctcg gcggtactac ccaatggcaa
    40201 tgcatcttca ttgtcgcgtt caatccagaa accttcttca caccacggaa ttggcgtaag
    40261 cgtccagcca taaggagcgg ttaattgcag gaaatcagca acggagattt tcagcgtatt
    40321 aacgcgaatg ctgcggcgca acgggcgctg acaggcggca agaaaatcat caaatgagag
    40381 cgtcgaaggc atcgcttcgc gcatttgtgt cagaaaggcg tccgggaaat aaacggtgtg
    40441 ttgggccacg agcatgtacc actggcaaaa aaacaggcgc gcagtttagc ataaacgctc
    40501 cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagttccc cattcacgcc
    40561 actctttcgg ttcactttcc tgcaacagga agtgtttgcc ttcctgggct ttcggtgcca
    40621 gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgc ttaaaggtgc
    40681 cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat gccaaccaga
    40741 agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc gcaatcatca
    40801 cgcgatctga caatgtcccc agcgtcattc ctgtaaccgt accgacttcc agaccacgga
    40861 acagcacagg cgtaccaata cctaacgaac cggcttccgg cgcttcaaca ataatgctta
    40921 agccatccag gtaacgcgaa tcagtaatgg tggcctcttg taattcaaag tcgcggcgag
    40981 gattgccccg gcctggttcg acgttgatat acggctggag gatagtatct agatgctcaa
    41041 cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg cgggcgaagg
    41101 tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc gcggtaatca
    41161 gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc atcccgaccg
    41221 ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca cgggccgctg
    41281 tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg gcaccgctga
    41341 ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc tgtacggtca
    41401 ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg ctattgctgg
    41461 tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg ttagctcgcg
    41521 gacggacggt aataacttca ccaacttcaa atttacggta gagcactacc gatcctgcct
    41581 gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa tcgctaaggc
    41641 tgttctccag cgctttttcc agattggcat acagtggata gctggctttc atctcgcctt
    41701 tatcgcccgg cagaatacgt atcccgccgt taatccattc tgaggcgctg gcaccgagaa
    41761 actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca aatttgctat
    41821 cgccttttac cagttctcga tgctgaggct cgatggcgac ggtaaaggtg acgcctttgc
    41881 tggtgagttt acgatcgata acctggccta cctgcacgcc gtgaagaatg agcggctgac
    41941 cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca tcaggttcat
    42001 gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg ccatcgccgg
    42061 gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg ctaagggata
    42121 atttcggatt acgtaattcg atgcgggtat tttcacgaag cagggtaacg acgctgggat
    42181 caactgtcat ttccccagtg actttaccac caggatttaa atccagttta gttagctgtc
    42241 cgacttccag cccctgatac attaacggcg tcgagtcggc ggtcaatccg gccccactcg
    42301 gcagttccag ttttattatt acgccacgct ggctgtgggc cagatcttca tacagaccaa
    42361 aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca atcgcaccgt
    42421 taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgacactg acgttggcat
    42481 caacgccgga aacgttccag aaacggctac cttttttcac cagatcggta aaacgccgct
    42541 cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag tcgtagactt
    42601 ttcccaccgg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa ccgagatcgg
    42661 gagcttgcag gtggatcatc agatcgccgt tatccagccg atacttcggt tgggtatcga
    42721 gtgcgacaaa gtgatcctgc tctttacctt tacccggcat catgccgata tagttcccac
    42781 cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc accagccaga
    42841 attgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc ttgacttcaa
    42901 tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca acgccctgat
    42961 aacgaacagg cgtacggccc gtaacaatac catccgccga cataaagtcg atggtgacgg
    43021 tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt agcgcgatga
    43081 aaggccgcag ccagaaaggt gagatacggc gtttattttt aatctgcgct tcagtcgtcg
    43141 aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag ccattccaca
    43201 gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc catagtaaaa
    43261 gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa caggtcgagc
    43321 atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt gcgtaaacct
    43381 tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac tttagtaaac
    43441 ggtaccagaa tactggcgat aaacacgatt cccgcaaccg caatgttgct acttgccagc
    43501 gacataattc cggaaagaat tgtatcttcc tgccgtcctc cgttcagata aatgatagaa
    43561 ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc cgcccagcat
    43621 ttttgcagac tatgacggcg acgcaggcgt agcgggatat ggcaacgcgg gcagcgacca
    43681 cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt ctcgtcccta
    43741 cgcgtagcgg ggcgctgcgg ataatatctc tcccacagct cctcaacatt aagatgtgac
    43801 aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac accagcctgg
    43861 atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag gtagatatcc
    43921 agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa attcattccc
    43981 agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg ggcaccaata
    44041 acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat ttgccagatg
    44101 ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa cagcggttcg
    44161 ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag gcgcgttagc
    44221 gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc actctgatga
    44281 gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg gtaatcacca
    44341 cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt aatttgtggt
    44401 gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatatt aacccatgat
    44461 tggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg cagttccagg
    44521 taagacaacc aagtgattat ataatgaaca aaacagaatt ttacgcggat ttaaatcgcg
    44581 actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg aacaccagtg
    44641 cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg cttgaggacg
    44701 atacactggt actaggacca tttcagggca aaattgcctg tgtccggata cccgtcgggc
    44761 gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag gatgttcacg
    44821 cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt ctgccgctgg
    44881 tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc ggtcgcttta
    44941 cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa gtgcttgcaa
    45001 cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat aacgtagcat
    45061 ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg ctacgtccgt
    45121 tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa gaagtaatcg
    45181 cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg cgtccgctga
    45241 aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac ctgagcaaaa
    45301 cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt tacggtgtta
    45361 aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg gacgagcaac
    45421 atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag gcacagcgtg
    45481 ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa gaagacgcac
    45541 cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc gctgaacgta
    45601 aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca cctcgcgaag
    45661 aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc ctgaaggtga
    45721 aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagac ggcgtccgcg
    45781 tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg ttctgaaacg
    45841 gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg cctgcttgca
    45901 atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat tccggtatta
    45961 aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt cacccgttct
    46021 cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga ccgctacctg
    46081 aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca gttcgcgaaa
    46141 aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt ctacgatctc
    46201 tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc ggtactggaa
    46261 aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa agcgccctgg
    46321 ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt cgacgagtta
    46381 agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac acgccgctac
    46441 aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc gctggcaatg
    46501 acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg taataccgaa
    46561 cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct gcaaatggat
    46621 gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa gagtaaagct
    46681 atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat ggttgacgtg
    46741 attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg cagtaaagtt
    46801 cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac attgacccgt
    46861 gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt cggtaaagag
    46921 aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga tgtcaaagtt
    46981 caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct gcgtagcaat
    47041 ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc tgcgggtccc
    47101 attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac cgacgggcag
    47161 gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc ggcttcagaa
    47221 atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga accgacgttt
    47281 ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca gatgttacgt
    47341 cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta tcgcgttaac
    47401 ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac gggtaatgaa
    47461 gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag cattgatgcc
    47521 gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct gaaggaacat
    47581 aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat cgcgcgcttc
    47641 aacgctatga aggacaagcg caatatcgtt tctctgaact acgctgtgcg tgagaaagag
    47701 aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa acgcgaaggt
    47761 aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc ggatccttat
    47821 ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc cagacccgcg
    47881 gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc ctgatttttt
    47941 aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt ctttctggtg
    48001 acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg ggtatcgcat
    48061 attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg ctcttcctgc
    48121 taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca gggatacagt
    48181 cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt ggttccttcg
    48241 tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa gtgatcgagc
    48301 aaccgcgtaa cgaaagggaa cgttggctgg ttaatactgt agcaacccag gctcgtcagg
    48361 cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac gcttttgcaa
    48421 ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg cagaacatga
    48481 gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc aatggtgata
    48541 tggttaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt atttcccgta
    48601 ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt gaagagagca
    48661 acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg tttggtattc
    48721 tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct gatgccggtt
    48781 cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg aaaaccagct
    48841 atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag tcgaaatcgc
    48901 tcagtgagtt gttcatgact cacccgccgt tggataaacg aattgaagct ctgcgtacgg
    48961 gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgtcg acgcgctttt
    49021 tttatgccct ggatcgtggc tgagtgatac gtaaaccact cacacaggca gcaatcactg
    49081 ccagaatagc cgcagccatc agcgaaacgt gcgtaccatt atcaccgaac tgatttagca
    49141 tcagcgccac cagcgccgcg ccactactct gacccagtag acgagccgtt cctaacatgc
    49201 cactggcccc accgctacgt tcgcgcggag cggaggtaat aatggtgtgg ttattgggtg
    49261 actggaataa gccaaatcca gcgccacata agatcatcgg ccagataata ttgatatccg
    49321 caggtgacgc gggcagcaga accagggaaa aaagccccgc agccatgatg aacaacccta
    49381 aagcaccctg caatcctgca tgtacgcgtt caatcaaata gcctgccagc ggagccatca
    49441 ccatcgttgc taacggccac ggtgtcagaa gtaaacctgt ttcgacttca ctacgcccga
    49501 gcatggtttg caggtaaaag ggcagggaaa ccattgccag catttgtgcg cagaaagagc
    49561 aaacagatgt gcaaatagaa agtgaaaaca gcgggatacg cagtaaatcc accggtagca
    49621 gcggtacggg aagagaaagc tggcggcgaa taaagaaaat accaacaaca accattacca
    49681 ccagttccgc agcaattaac gtcagcgatt gcccctgagc gaaaccactc aacgcagtga
    49741 taagcaggcc gaaggttaac gcgttcatca cggcgctggg caggtcgaaa cggggtttac
    49801 tggcgcgaga accattgggt ggcagaaaac gcatcgccag aagcagggcg ataataccta
    49861 acggtacgtt gattaaaaat aaccatttcc aggatgcgat ggagaggatt gctgcagcaa
    49921 ttgtcggccc ggcagcagaa gagacggcaa caataaacga gtttatgccc atccctctcc
    49981 ccagaaaacg ttgtggatag atcaggcgga taagtgcggt attaacgctc atcaacgctg
    50041 caccgccgaa accttgtatg acacgcgcaa gggtgagcat ttgcagcgaa tcagaaaggg
    50101 cgcagaacag tgaagacaac agaaaaacga ccagaccgca tttataaata cgtcgatagc
    50161 caaacatatc gcccagaaac gaaaacgaga gcagggagat gacaatggcg atttgatagg
    50221 cgttcactac ccagatggaa ctggctggcg tggcatgaag gtccgtggcg attgttggca
    50281 gggcgacgtt ggcgattgcg ccgtcaagga cagccatcga aataccaatc acaatggtta
    50341 atatcgcacc gtatcgctgg ggcaatggca ggccgtcggc ctgaactttt ggcataggaa
    50401 ttttatatct ttggtgaata atcaacaggg gtatgattct agcactgata ttcacggtgg
    50461 atgtcgcaga attgtaacta accggcaaaa gatggattgc gaccaccctg gtgcgaaatt
    50521 ataataaaaa acagttctga tttttataaa acactcgcaa tgaggtgata aatggctaac
    50581 gcagatctgg ataaacagcc tgattctgta tcttccgtgc taaaagtttt tggcattttg
    50641 caggcgctgg gtgaagagcg cgaaataggg ataaccgagc tgtcgcagcg cgtcatgatg
    50701 tcaaaaagca ccgtttatcg ctttttacag accatgaaaa ccttaggtta tgtggcgcag
    50761 gaaggggagt cggagaaata ttccctgacc ctgaaattgt ttgaactggg cgctcgcgcg
    50821 ttacaaaacg tcgatttaat tcgtagcgca gatatccaga tgcgtgagat ctcccgcctg
    50881 accaaagaaa ctatccacct cggcgcactg gacgaagaca gtattgttta cattcacaaa
    50941 attgactcta tgtacaattt gcgcatgtat tcacggattg ggcgtcgtaa tccgctgtac
    51001 agcaccgcga ttggtaaggt actgctggca tggcgcgatc gcgatgaagt gaagcaaatt
    51061 cttgagggcg tggagtataa acgcagtacc gagcggacca tcaccagtac agaagcgtta
    51121 ttacccgttc tggaccaggt gcgcgagcag gggtatggcg aagataacga agagcaggaa
    51181 gaagggctgc gatgcattgc ggtaccggta tttgatcgct ttggcgtggt cattgccggt
    51241 ttgagcatct ccttcccgac gttgcgtttc tctgaagagc gtttacagga atatgtcgca
    51301 atgttgcata ccgcagcgcg caaaatttct gcccaaatgg gttatcacga ctatccgttc
    51361 tgatgagagt aagaacctgt cggaatatca aacagacagg ttctttattt agcatgagaa
    51421 aaataaagtt gaaggtgcgt tatattaaac gcgcttgcta taagaatatt ttactcatga
    51481 gtgagaatct ggttatttat tacccttaac cattatcgac cacgatattg cttttacgta
    51541 acagtgggca atctgttatc cccaagaaac cacttttagt gtgcaagtat tgtaccgtgc
    51601 tggtgcctct ggcagtcaga taggtacatt gcaaacctaa tcctgcggca ttctctttgc
    51661 ttccaatcaa aacgccatat ccgctgagta ataatcctat ccataccagt gctatcagca
    51721 taactgtgcg aatgatgaat cgcattacaa cctcttctct ttttatgttc gcttaatcgt
    51781 agcggcaata tgcgctgaag caagctactc attccgaaaa agcacgaata tcgacatagt
    51841 taggcgctgt ttaactaacg catgctagtt taatgacata aggtaggtga agcggagatt
    51901 ggagtgaaaa agtttcgatg ggtcgttctg gttgtcgtgg tgttggcttg cttgctgctt
    51961 tgggcgcagg tattcaacat gatgtgcgat caggatgtac aatttttcag cggaatttgt
    52021 gccattaacc agtttatccc gtggtgatat cattttttca gcactagatt ttcttcctct
    52081 ccagcagtgg tagaatagcg cctctactca gaggtggtga aatgaacgaa gttgtaaatt
    52141 caggcgtgat gaacattgcg tctttggttg tatcggtggt ggttcttctt atcgggctca
    52201 tcttgtggtt ttttatcaat cgtgccagtt ctcggactaa cgaacagatt gaactgcttg
    52261 aggcgttgct ggatcagcaa aaacgtcaaa atgcactgtt acgtcgtttg tgcgaagcaa
    52321 acgaacctga gaaagcagat aaaaagaccg ttgagagtca aaaatcggtt gaagacgaag
    52381 atattattcg tctggtcgcc gaacgataat ccttcctcaa tcttgagtaa ccccgtcgtt
    52441 gaagggttac tcaattcatc ctgattatac gcactgccat ggtgcaactg cttcaaatta
    52501 aaactctggt ctgacttcaa ataattatca tattatttat ttgcgtaacg ggatatcaga
    52561 aaaagatatt ttc
//
LOCUS       DAZGXZ010000015        51620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000015 DAZGXZ010000000
VERSION     DAZGXZ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.015"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            232..651
                     /gene="umuD"
                     /locus_tag="ACHKJF_001032"
     CDS             232..651
                     /gene="umuD"
                     /locus_tag="ACHKJF_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0948433.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            651..1919
                     /gene="umuC"
                     /locus_tag="ACHKJF_001033"
     CDS             651..1919
                     /gene="umuC"
                     /locus_tag="ACHKJF_001033"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP0948434.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1965..2495)
                     /gene="dsbB"
                     /locus_tag="ACHKJF_001034"
     CDS             complement(1965..2495)
                     /gene="dsbB"
                     /locus_tag="ACHKJF_001034"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP0948435.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2641..4182)
                     /gene="nhaB"
                     /locus_tag="ACHKJF_001035"
     CDS             complement(2641..4182)
                     /gene="nhaB"
                     /locus_tag="ACHKJF_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP0948436.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4404..5123
                     /gene="fadR"
                     /locus_tag="ACHKJF_001036"
     CDS             4404..5123
                     /gene="fadR"
                     /locus_tag="ACHKJF_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP0948437.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5175..6707)
                     /gene="ycgB"
                     /locus_tag="ACHKJF_001037"
     CDS             complement(5175..6707)
                     /gene="ycgB"
                     /locus_tag="ACHKJF_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP0948438.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7037..8335
                     /gene="dadA"
                     /locus_tag="ACHKJF_001038"
     CDS             7037..8335
                     /gene="dadA"
                     /locus_tag="ACHKJF_001038"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP0948439.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8345..9415
                     /gene="dadX"
                     /locus_tag="ACHKJF_001039"
     CDS             8345..9415
                     /gene="dadX"
                     /locus_tag="ACHKJF_001039"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP0948440.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9801..11537)
                     /gene="cvrA"
                     /locus_tag="ACHKJF_001040"
     CDS             complement(9801..11537)
                     /gene="cvrA"
                     /locus_tag="ACHKJF_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP0948441.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11632..12546)
                     /gene="ldcA"
                     /locus_tag="ACHKJF_001041"
     CDS             complement(11632..12546)
                     /gene="ldcA"
                     /locus_tag="ACHKJF_001041"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP0948442.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12646..13257
                     /gene="emtA"
                     /locus_tag="ACHKJF_001042"
     CDS             12646..13257
                     /gene="emtA"
                     /locus_tag="ACHKJF_001042"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP0948443.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13259..13993)
                     /gene="ycgR"
                     /locus_tag="ACHKJF_001043"
     CDS             complement(13259..13993)
                     /gene="ycgR"
                     /locus_tag="ACHKJF_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP0948444.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14194..14448
                     /gene="ymgE"
                     /locus_tag="ACHKJF_001044"
     CDS             14194..14448
                     /gene="ymgE"
                     /locus_tag="ACHKJF_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0948445.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14626..15066
                     /gene="ycgY"
                     /locus_tag="ACHKJF_001045"
     CDS             14626..15066
                     /gene="ycgY"
                     /locus_tag="ACHKJF_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFP0948446.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15145..16842)
                     /gene="treA"
                     /locus_tag="ACHKJF_001046"
     CDS             complement(15145..16842)
                     /gene="treA"
                     /locus_tag="ACHKJF_001046"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0948447.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWEEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17162..18580)
                     /gene="dhaM"
                     /locus_tag="ACHKJF_001047"
     CDS             complement(17162..18580)
                     /gene="dhaM"
                     /locus_tag="ACHKJF_001047"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP0948448.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18591..19223)
                     /gene="dhaL"
                     /locus_tag="ACHKJF_001048"
     CDS             complement(18591..19223)
                     /gene="dhaL"
                     /locus_tag="ACHKJF_001048"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP0948449.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19234..20304)
                     /gene="dhaK"
                     /locus_tag="ACHKJF_001049"
     CDS             complement(19234..20304)
                     /gene="dhaK"
                     /locus_tag="ACHKJF_001049"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP0948450.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDTPVHTPALNWGK"
     gene            20532..22451
                     /gene="dhaR"
                     /locus_tag="ACHKJF_001050"
     CDS             20532..22451
                     /gene="dhaR"
                     /locus_tag="ACHKJF_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP0948451.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMSPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDVQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22551..25436)
                     /gene="ycgV"
                     /locus_tag="ACHKJF_001051"
     CDS             complement(22551..25436)
                     /gene="ycgV"
                     /locus_tag="ACHKJF_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0948452.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPDPTPNPTPTPKPTTTADAGGNYLNVGYLLNYVENR
                     TLMQRMGDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMP
                     LYVGLYIGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVL
                     DSQNNGVNANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNG
                     LNIHLNHYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYS
                     FKGNGWNNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(26277..27368)
                     /gene="ychF"
                     /locus_tag="ACHKJF_001052"
     CDS             complement(26277..27368)
                     /gene="ychF"
                     /locus_tag="ACHKJF_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP0948453.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27485..28069)
                     /gene="pth"
                     /locus_tag="ACHKJF_001053"
     CDS             complement(27485..28069)
                     /gene="pth"
                     /locus_tag="ACHKJF_001053"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP0948454.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28347..28625
                     /gene="ychH"
                     /locus_tag="ACHKJF_001054"
     CDS             28347..28625
                     /gene="ychH"
                     /locus_tag="ACHKJF_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP0948455.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28680..30359)
                     /gene="dauA"
                     /locus_tag="ACHKJF_001055"
     CDS             complement(28680..30359)
                     /gene="dauA"
                     /locus_tag="ACHKJF_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP0948456.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30484..31431)
                     /gene="prs"
                     /locus_tag="ACHKJF_001056"
     CDS             complement(30484..31431)
                     /gene="prs"
                     /locus_tag="ACHKJF_001056"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP0948457.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31582..32433)
                     /gene="ispE"
                     /locus_tag="ACHKJF_001057"
     CDS             complement(31582..32433)
                     /gene="ispE"
                     /locus_tag="ACHKJF_001057"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP0948458.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32433..33056)
                     /gene="lolB"
                     /locus_tag="ACHKJF_001058"
     CDS             complement(32433..33056)
                     /gene="lolB"
                     /locus_tag="ACHKJF_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP0948459.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33270..34526
                     /gene="hemA"
                     /locus_tag="ACHKJF_001059"
     CDS             33270..34526
                     /gene="hemA"
                     /locus_tag="ACHKJF_001059"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP0948460.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34568..35650
                     /gene="prfA"
                     /locus_tag="ACHKJF_001060"
     CDS             34568..35650
                     /gene="prfA"
                     /locus_tag="ACHKJF_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP0948461.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35650..36483
                     /gene="prmC"
                     /locus_tag="ACHKJF_001061"
     CDS             35650..36483
                     /gene="prmC"
                     /locus_tag="ACHKJF_001061"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0948462.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36480..36872
                     /gene="sirB2"
                     /locus_tag="ACHKJF_001062"
     CDS             36480..36872
                     /gene="sirB2"
                     /locus_tag="ACHKJF_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP0948463.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36876..37685
                     /gene="sirB1"
                     /locus_tag="ACHKJF_001063"
     CDS             36876..37685
                     /gene="sirB1"
                     /locus_tag="ACHKJF_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP0948464.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37721..38575
                     /gene="kdsA"
                     /locus_tag="ACHKJF_001064"
     CDS             37721..38575
                     /gene="kdsA"
                     /locus_tag="ACHKJF_001064"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP0948465.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38724..38831)
                     /gene="ldrA"
                     /locus_tag="ACHKJF_001065"
     CDS             complement(38724..38831)
                     /gene="ldrA"
                     /locus_tag="ACHKJF_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0948466.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(39276..40013)
                     /locus_tag="ACHKJF_001066"
     CDS             complement(39276..40013)
                     /locus_tag="ACHKJF_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFP0948467.1"
                     /translation="MGNRKQVTSRIISTPELIRYNDNIVGYGSRELRVETISCWLARL
                     VIVNKHYSHRFVNNSYLHLGIFSERELVGVMQWGYALNPNSGARVVTGTQNREYMELN
                     RLWMHDCMPRNSESRAISYALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVG
                     SHESTFYELDGEWYHEICRSAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRAR
                     KRLNTKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(39967..40167)
                     /locus_tag="ACHKJF_001067"
     CDS             complement(39967..40167)
                     /locus_tag="ACHKJF_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0948468.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSPSATEHP
                     TYTRKSCRGEQKASHIPYHQHA"
     gene            40282..40744
                     /locus_tag="ACHKJF_001068"
                     /pseudo
     CDS             40282..40744
                     /locus_tag="ACHKJF_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(40783..41028)
                     /locus_tag="ACHKJF_001069"
     CDS             complement(40783..41028)
                     /locus_tag="ACHKJF_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0948469.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGRSPAENSTENEPQPVEQPEPVQPGTDGTMM"
     gene            complement(41064..41243)
                     /locus_tag="ACHKJF_001070"
     CDS             complement(41064..41243)
                     /locus_tag="ACHKJF_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0948470.1"
                     /translation="MTYINVCLLYFCTVVCCLLLVSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            complement(41301..43358)
                     /locus_tag="ACHKJF_001071"
     CDS             complement(41301..43358)
                     /locus_tag="ACHKJF_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0948471.1"
                     /translation="MNIMTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMT
                     FADCLISFNTQDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDWDNAETHT
                     RPAGNETASSSPSWYYVIVIAGQSNASSYGEGLPLPDSYDRPDPRIMQLARRNTQTPG
                     GIPCKYNEIIPADHCLHDVQNMSLLNHPKADLKKGQYGCVGQGLHIAKKVLPVIPADA
                     GILLVPCARGGSAFTTGAVGSFDPASGAAEASLRWGVDTPLYQDLVSRTKAALEANPK
                     NVLLSVVWIQGEGDLKATPAQHGAQFTAMVKKFRADLGAFSGQCIGGSSDSVPWLCGD
                     TTYDWKEKNPAAYETIYSGYKNRASENIHFVPLMHDEFGNKTATNLPEHDPDIASAGY
                     YGAESRGDGNWTTASRATHFSSWARRFLVSDRFASAILKYAGLTSAFIAGVPPDGEAL
                     PQPAPGGKPEAPTGSQPGGSESPHPTSPSLRTLLSLKASAGEAPAQGWKITDGTIQLT
                     DEGKALTVSKQSGKTWTLTHGTDAAGELLKHGGQLTCKFRLSGEVKEGQFALGIYLHT
                     DTVPEGVTMSGTGTPFLMAYYLQTTGGQLNLMHHKTGGNTKLGSFGAYNNQWHTLVLK
                     FTAGSAQVTPVLDGTPGQAFQMVKDALSPAKDTLTLTDVTKGATYGVSFESVVLEVNT
                     PAA"
     gene            complement(43449..44009)
                     /locus_tag="ACHKJF_001072"
     CDS             complement(43449..44009)
                     /locus_tag="ACHKJF_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0948472.1"
                     /translation="MLIGYIRVSTNDQNTGLQRNALECAGCEQIFEDKMSGKTTQRPG
                     LKRALKKLKPGDTLMVWKLDRLGRSMRHLVALTDELQQQGINFRSLTDNIDTSTPMGR
                     FFFHIMGALAEMERELIVERTRAGLAAARERGRTGGRPRRLTQEQYEQVGRLLAGGVS
                     RRQVALIFDIGLSTLYRYYPASVSEK"
     gene            complement(44023..44208)
                     /locus_tag="ACHKJF_001073"
     CDS             complement(44023..44208)
                     /locus_tag="ACHKJF_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948473.1"
                     /translation="MFISCVPSKATPLILRAFASLLAATAVPFDGRAFAALFTVSRKP
                     RFQGCRISALKGLSISF"
     gene            44565..45086
                     /locus_tag="ACHKJF_001074"
     CDS             44565..45086
                     /locus_tag="ACHKJF_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0948474.1"
                     /translation="MQLINVKRYYPKHKPYGEDVQYFQSEDGRDFYESIPLFTKKYKL
                     CISPVTGIICSVAEDVSALYPAGFTVVEVDELPEGVNIDGNWQFSDGLISKVPVNWKT
                     VAEKRRSSLLQEANETVDDWKTELKLDMISDENKLQLTRWMAYIRQLKEMHFNDIASE
                     GHYQAIPWPEKPE"
     gene            complement(45121..46032)
                     /locus_tag="ACHKJF_001075"
     CDS             complement(45121..46032)
                     /locus_tag="ACHKJF_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0948475.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGALATKNSLGPGDVNALAKDQNLADLENAGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNKAADALPSGGTAVAANRLATPRNINGVPFDGTQD
                     INITSGMTQSTADGRYVQNVQLGAQSYHSPGGNEISWNYSAPSGCMLSGINVQETGSR
                     SADNIGGVYYRPVQIYINNAWRTVSSV"
     gene            complement(46032..46592)
                     /locus_tag="ACHKJF_001076"
     CDS             complement(46032..46592)
                     /locus_tag="ACHKJF_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFP0948476.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWTGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYEIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            complement(46583..47665)
                     /locus_tag="ACHKJF_001077"
     CDS             complement(46583..47665)
                     /locus_tag="ACHKJF_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0948477.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNTPTGTRLSFITPQAGINQ
                     TATVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCKP
                     EWPEAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FEVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(47665..48102)
                     /locus_tag="ACHKJF_001078"
     CDS             complement(47665..48102)
                     /locus_tag="ACHKJF_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFP0948478.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPVGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHARLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(48095..48709)
                     /locus_tag="ACHKJF_001079"
     CDS             complement(48095..48709)
                     /locus_tag="ACHKJF_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0948479.1"
                     /translation="MMNDEVISRLLAPVMRGVRLLFGRGVLTGTTDTLKIQNVQITGM
                     DGETFDDVERPQQYGQISVPLPGAEVFLACAGGQRDQAVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKNGRIIVTCKTLEIYADEGVQVDTPEAHFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(48699..49823)
                     /locus_tag="ACHKJF_001080"
     CDS             complement(48699..49823)
                     /locus_tag="ACHKJF_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFP0948480.1"
                     /translation="MSSRVELYLGGEIFSGWLTVSVRRSLEHLAGSFELGVMMPGVRL
                     PSSVRAGQSLELRIDGQPVITGWLDQVRQRISATRFQITLSGRDKTGDLVDCSAIHPG
                     SQWRNRTLEHIASDLCAPFGVTVRWQVNDATAARPFSTFTLENSETVADALTRAARHR
                     GVLVTSNAAGELVFTQAGSQRGDTLTLGENLLDLDHNVDHRLRYSEYRVRGHGRGGGH
                     AGDALTAGTLAAPVGTVTDSAIHRYRPKIVLADHAVDADGARQRAVREMRRAVARSVR
                     LTATVRHWFRENGQLWDINLLTAVTAPRTGVEERDLLVCQVEFSLDANHGETTRLILA
                     PRDGFIVPAEPGNSGSGNAGDVDAFVRAQMKKQGIKFNDE"
     gene            complement(49807..51156)
                     /locus_tag="ACHKJF_001081"
     CDS             complement(49807..51156)
                     /locus_tag="ACHKJF_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFP0948481.1"
                     /translation="MASGDGRGAFRGVPFLVWREQRERGGRNIVRREYPLRETGGADD
                     LGPKLAEFTFSVLVMGDDVQTQRNRLRDALRAPGAGELLHPDYGTLNVLINSFESRYN
                     AAEQGVVEFTINVTPVSDDTAPAVTQDTAAILEQKSTTALGKVFETLEAGWTVISDGM
                     HDVQAMTETISDKVSALENAVSGMGIVQDISAFTATFTALKGNATALLTAPSRMASSF
                     AGLFSALITLPSLPSLSSGGLNTRPGGSIGRTTSAVSQGMPQLYRTLSSLRYVLDEQD
                     NPQTLIGLTPAAQKNIRLIRAVMQSAAVVAQAQTVGKLLDQVLSQETLPDSDAAHRTW
                     PVWLESSVDLQRINRDLSEALERQVMTLSGQGYTATALTLRDARLALTEDLNTRGVQL
                     PGATVVTVRTTEPALVTLYRATGNSTGWQRFVRRNGIVDPLFIPGGHSVEVISEQQG"
     gene            complement(51143..>51620)
                     /locus_tag="ACHKJF_001082"
     CDS             complement(51143..>51620)
                     /locus_tag="ACHKJF_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFP0948482.1"
                     /translation="LLSSVGSRMGLGRFSGFFRSAGGLAGRLGGGALWAGAMAAPVLL
                     DGSASATDKGEAVGSLAGSIAGGALGAAAGPVGIAIGSTVGSYLGNYLGGWLTEAWQK
                     LRGSDDESSGQAVQKASARVELVAPEGWQARSIDIDDTSGHGLDVNVWNGGNYGLW"
BASE COUNT        12664 a        13218 c        13109 g        12629 t
ORIGIN      
        1 caatgtttaa tgtcattatg gcgaatgctt ctattctatt tttttgccgg gtgatatttt
       61 tcatttctgc tggatgagcg tcgtcgccag aaggccacgt gagcacagga taagagaacg
      121 aaaaatcagc agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg
      181 gcaagaacag actactgtat ataaaaacag tataacttca ggcagattat tatgttgttt
      241 atcaagcctg cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag
      301 tgtggctttc cttcaccggc agcagattac gttgaacagc gcatcgatct gaatcaactg
      361 ttgatccagc atcccagcgc gacttacttc gtcaaagcaa gtggtgattc tatgattgat
      421 ggtggaatta gtgacggtga tttactgatt gtcgatagcg ctattaccgc cagccatggt
      481 gatattgtca tcgctgctgt tgacggcgag tttacggtga aaaaattgca actacgcccg
      541 acggtacagc ttattcccat gaacagtgcg tactcgccca ttaccatcag tagcgaagat
      601 acgctggatg tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgctc
      661 tctgtgatgt aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg
      721 gtaaaccggt ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg
      781 caaaggcgct gggtgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc
      841 gctgtggcgt ggtttgcttt agcagcaatt atgagcttta cgcagacatg agcaatcggg
      901 tgatgtcgac gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat
      961 tctgcgatct gacaggtgtg cgtaattgtc gcgatctgac tgattttggc agagaaattc
     1021 gcgcaacggt gctacaacgt acccatctta ctgttggtgt ggggatcgcc cagaccaaaa
     1081 cgttggctaa gcttgccaat catgcggcaa aaaaatggca gcggcagacg ggtggggtgg
     1141 tggatttatc aaatctggaa cgccagcgta aattaatgtc tgctctcccc gtggatgacg
     1201 tctgggggat tggacggcgg atcagcaaaa aactggacgc gatggggatc aaaaccgttc
     1261 tcgatttggc ggatacagat atccggttta tccgtaaaca ttttaatgtc gtgctcgaaa
     1321 gaacggtgcg tgaactgcgc ggcgaaccct gtttgcaact ggaagagttt gcaccgacga
     1381 agcaggaaat tatctgttcc cgctcgtttg gtgaacgcat cacggattat ccatcgatgc
     1441 ggcaggccat ttgtagttac gctgcccggg cggcggaaaa acttcgcagc gagcatcaat
     1501 attgtcggtt tatctccaca tttattaaga cgtcaccatt tgcgctcaat gaaccttatt
     1561 acggcaatag cgcgtcggta aaactgctga cgcccactca ggacagcagg gatatcatta
     1621 acgctgctac gcgatctctg gatgccatct ggcaagcggg ccatcgctac caaaaagcgg
     1681 gcgtgatgct gggggatttc ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca
     1741 acgcaccgcg ccccgggagt gagcaattga tgacggtaat ggatacactg aatgctaaag
     1801 agggcagagg aacactctat tttgccgggc aggggatcca gcaacaatgg cagatgaagc
     1861 gtgccatgct ttcaccacgt tatacaacgc gaagttctga tttactgcgg gtcaaataaa
     1921 tatagcggca ggaaaaaagc gctcccgcag gagcgctgaa gggattagcg accgaacagg
     1981 tcacgttttt tcgctttaaa cggctgggaa atcaccacca gcaccgcgac aatcaggtaa
     2041 gcgataaaaa taccgagcag ccactgcggc atttccagac ctaaaaaatc ccactgacgc
     2101 tcggcacaat cgccagaggc gacaaacact tgcggcaccc atttatccag cggcagccat
     2161 tccgggaaac gaaccataaa atcacaggtg gcaaacggcg aaggatagag ctgaagcatg
     2221 gtgtgctcgt aagttaactg cacaccgcgg aacgcactat acaaccagat aaccatcgct
     2281 acataacgca gcggagtttt cggggcgatc gcgccaatca gcgcagcacc cagaacgccg
     2341 aataacgcac aacgttcata aatacagagc acgcaaggtt tcagtaacat cacatgctgg
     2401 aaccacagcg ccgtcagttc cagtgccaga gcagtaaacg ccatcaacag ccacgcgccc
     2461 cggccttgtg aacattggtt caaaaatcgc aacataatca tttccctgca atatgcatag
     2521 agtgcgcagt ttaaaccaat tcattcgctg cgccaccagg aggcagcaaa aataggaagc
     2581 aattggacaa ttatcgggcg aaaaggcaaa ccgggcattt atatgcccgg taagttgtta
     2641 tcaaagcgtt gctatccagc ccatttgcat aaaccattcg gttacagggg caagcgtaaa
     2701 ctcgacgcag agcaagccga cgagtgtcag tacgagggtg taaggcaggg ccatccacac
     2761 catgcggcca taagagaggc gaatcaatgg cgcgagtgca gaggtcagca ggaacaggaa
     2821 cgcagcctga ccgttcggcg tagcgacgga gggcagattg gtaccggtat taatggcgac
     2881 cgccagcagc tcgtattgct tcaacgtgat agcgccactt tccattgccg ctttcgcttc
     2941 gttgatataa atcgtcccca cgaagacgtt atccgaaatg gatgacagca gaccgttgaa
     3001 aatatagaac agcgacagct gagcatgttc cgatgcctgc aacacaaact gaataattgg
     3061 cgaaaacagt tgttggtcga taatcaccgc gactaccgag aaaaacaccg tcaacagtgc
     3121 ggtgaatggc agagattcgg tgaaggcttt accgatagca tgttcatcgg tgacaccggt
     3181 caatgatgtc gccagaataa tgactgacaa accaatcaag ccaacttccg ccagatgcaa
     3241 cgccagcgca gtcaccagcc agacgccaat aatcgcctgg acaatcagac ggattttatc
     3301 ctgacgggta cgctggtggc ggctttgatc gtcaaactgt tgcaacactt cgcggacttt
     3361 ctccggcagc gtttcaccgt agccaaacca acgcagcttc tctaccagca ggcaggttaa
     3421 caggccacaa atcagaaccg gaacggtcac cggcgacatg cgcaggaaga aatcgccaaa
     3481 atgccagcca gccgctttag cgatgatcag gttctgtggt tcgcccacca tggtcatcac
     3541 gccgcctaat gcagtgccga caccggcatg catcatcagg ctacgcagaa agccacggaa
     3601 ctgttccaga accactttgt aatgcttgtc gatatgacta tcgtcttgca ggtcggtatc
     3661 ttcggtacgg gaagaggcta cgcggtgata aataccataa aaaccgacag caacgctgat
     3721 caccaccgcc acgacggtta aggcatcgag gaacgcggag aggaacgctg ccgccacgca
     3781 aaaagagagc gacagcagca ttttggagcg aatgcttaac agcaaacggg taaatatgaa
     3841 cagcaacagc tgtttcataa aatagatacc cgccaccata aacatcaata acagcaagac
     3901 ttcaagattt gccgccacct cttcacggac gtgttccgcg ctggtcatgc cgatgaatac
     3961 cgcttcgata gccaacagac caccggggag cagcgggtag catttcaggg ccatcgccag
     4021 agtgaaaata aattccgcta ccagcaacca gcccgcgacg aaagggctga tgaggaaaat
     4081 taacgggttt acgattaaga aaatgatgag ggcgagtttg taccagtcgg gggactggcc
     4141 caaaaaattg cgccatagcg cgcggcccca ggagatctcc atgatggttt cccttacctt
     4201 acaaataatc aatgatgttt ttatgtttaa acgcaaagct taacggtcag gcaggagtga
     4261 ggcaagtctt gatagtcaag gggaaagaga tgcggaaaat gaagccttga tccctttttt
     4321 cttctttttg tctgctatca gcgtagttag ccctctggta tgatgagtcc aactttgttt
     4381 tgctgtgtta tggaaatctc actatggtca ttaaggcgca aagcccggcg ggtttcgcgg
     4441 aagagtacat tattgaaagt atctggaata accgcttccc tcccgggact attttgcccg
     4501 cagaacgtga actttcagaa ttaattggcg taacgcgtac tacgttacgt gaagtgttac
     4561 agcgtctggc acgagatggc tggttgacca ttcaacatgg caagccgacg aaggtgaata
     4621 atttctggga aacctctggt ttaaatatcc ttgaaacact ggcgcgactg gatcacgaaa
     4681 gtgtgccgca gcttattgat aatttgctgt cggtgcgtac caatatttcc actattttta
     4741 ttcgcaccgc gtttcgtcag catcccgata aagcgcagga agtgctggct accgctaatg
     4801 aagtggccga tcacgccgat gcctttgccg agctggatta caacatattc cgcggcctgg
     4861 cgtttgcttc cggcaacccg atttacggtc tgatccttaa cgggatgaaa gggctgtata
     4921 cgcgcattgg tcgtcactat ttcgccaatc cggaagcgcg cagtctggcg ctgggcttct
     4981 accacaaact gtcggcgttg tgcagtgaag gcgcgcacga tcaggtgtac gaaacagtgc
     5041 gtcgctatgg gcatgagagt ggcgagattt ggcaccggat gcagaaaaat ctgccgggtg
     5101 atttagccat tcaggggcga taatcccttc cgtttaaaga gcaaacccct caaacgaggg
     5161 gttttttgtt gtttttacag atttcccatt cttggcgggc aacgttccag caactcgatg
     5221 ctgccgtctt cgttttgctg ttcgagcatc acatcaaatc cccacaggcg atgcacatgc
     5281 ttcagcactt ctttgcgccc ccgatccagc ggtgcgcgat tatgtggaat gtaacgcagc
     5341 gtcagcgaac ggtcgccgcg caaatccacg ttccagatct gaatattcgg ctccagatta
     5401 cttaagttat attgcgacga taaccggtta cggatctccc gataaccttc ttcattatga
     5461 atagcggaaa tctccagata attatgccga tcatcatcca gcacggtgaa gaagcggaaa
     5521 tcacgcatca ctttcggtga cagaaactgg ctgataaaac tctcatcttt gaaatcacgc
     5581 atcgcgaaat gcagcgtttc cagccagtcg gaaccggcga tatccgggaa ccagtatttg
     5641 tcttcttccg ttggcgactg acaaatccgt ttaatatcct ggaacatggc gaacccgagg
     5701 gcatacgggt tgatgccgct gtaccacggg ctgttatagg ggggctggaa gaccacattg
     5761 gtgtggctgt gcaaaaactc cagcataaaa cgttccgtta ctttcccttc atcatacaga
     5821 tggttaagga tggtatagtg ccagaaggtc gcccagcctt cgttcatcac ctgagtctgt
     5881 ttttgcggat aaaaatactg gctcacctta cgcacaatac gcaggatttc acgctgccat
     5941 gattccagca gtggcgcatt tttctccata aaatagagca ggttttcttg tggttcggac
     6001 ggatagcggc gcgcttcagc aaccgttttc tcttcctcgc gcttcggcag ggtacgccag
     6061 agcatattga cctgactttg tagatactct tcgcgacttt tctgccgggc tttctcttct
     6121 tgcagcgaga ttttttgcgg gcgtttgtac cggtccacgc cgtagttcat cagcgcgtgg
     6181 cacgagtcca gaagccgttc tacttcatca acgccataac gttcttcgca ctcggtaata
     6241 tatttacggg caaaaatcag ataatcgaca atcgaactgg cgtcggtcca actgcggaat
     6301 aagtaattgt ttttaaagaa agagttatgc ccatagcagg cgtgtgccat caccagcgcc
     6361 tgcatggtaa tggtgttctc ttccatcagg taagcgatac acgggttaga gttaatgacg
     6421 atttcatagg ccagtccttg ctgaccgtgc ttatacagcc gttcagtctc gataaacttt
     6481 ttaccgaatg accagtgcgg atagttaatt ggcatgccga cgctggagta ggcatccatc
     6541 atctgttctg aggttatcac ttcaatctgg tgcgggtagg tatccagccg gtagagtttc
     6601 gccacccggt ctatctctgc cagataaaca tccagcaggt cgaacgtcca gtcgggtcca
     6661 tcgctcaaac gtgtggtgtc cttattcata gaatcgatcg tcgccatacg cgcacctcat
     6721 tgttgtcggc gctctctgtg tggagcacct catttcaagc atagaacacc tgttaaaaac
     6781 cgcgtcgccg gagaattttt ttctttgcga tttcttatta tcagagtgcc actaatccgc
     6841 ttctgaacgg aattttatgc tggataaaaa gggcgttcag caggagatac taaagacgcc
     6901 atattgccgc agagtcaggg agatgtgagc cagctcacca taaaaaagcc gtatgttgaa
     6961 taatattttc aactgagtta tcaggatgta attagattat tattctttta ctgtatctac
     7021 cgttatcgga gtggctatgc gagttgtcat actgggaagt ggtgtggtag gcgttgccag
     7081 cgcctggtac ttaaatcagg caggacatga ggtcaccgtc attgatcggg agccgggggc
     7141 cgccctggaa accagcgccg cgaatgccgg gcaaatctcc cccggatatg ctgcaccgtg
     7201 ggcggcacca ggtgtgcctt taaaagcgat caaatggatg ttccagcgcc atgcgccgct
     7261 ggcggtccgt ctcgacggta cgcagtttca gttgaaatgg atgtggcaaa tgttacgtaa
     7321 ctgcgacacc agccactaca tggaaaacaa agggcggatg gtgcgtctgg cggaatacag
     7381 ccgtgattgc ctgaaagcat tgcgcgccga aaccaatatt cagtatgaag ggcgtcaggg
     7441 tgggacgctg caactgttcc gtaccgaaca acagtatgaa aatgcgaccc gcgatatcgc
     7501 cgtgctggaa gatgccggcg taccgtatca gctgctggaa tccagccgcc tggcggaagt
     7561 ggagcccgcg ctggcagaag tagcgcacaa actgacgggt ggtctgcaac tgcccaatga
     7621 tgaaaccgga gactgccagc tgtttaccca gaatctggcg cggatggcag agcaggcggg
     7681 ggttaaattc cgctttaata cgcccgttga ccaactgctt tgcgacggcg agcaaatcta
     7741 cggcgtgaag tgtggcgatg aagtgattaa ggccgatgcg tatgtgatgg cgtttggttc
     7801 ttactcgacg gcgatgctca aaggcattgt tgatattccg gtgtatccgc tgaaaggcta
     7861 ctcgctgact attccaattg cgcaggaaga tggtgcgcca gtatccacta ttcttgatga
     7921 aacctacaaa atcgccatta cccgtttcga taaccgcatt cgtgttggcg gaatggcgga
     7981 aattgttggt tttaacaccg agctgttgca accgcgtcgt gaaacgctgg aaatggtggt
     8041 tcgcgatctc tatccacgcg gcggtcatgt cgagcaggcg actttctgga cgggtttgcg
     8101 cccgatgacg ccagacggta cgccggttgt cgggcgtaca cgctttaaaa atctgtggct
     8161 gaataccggt cacggcacgc tcggctggac gatggcttgc ggttccggtc agttgttaag
     8221 cgatctgctc tctggtcgca cgccagcgat cccatatgag gatctaagcg tagcgcgcta
     8281 cagccgtgga tttacgccat cacgtccggg ccatttacat ggcgcacaca gctaaggaaa
     8341 cgagatgacc cgtccgatac aggccagcct cgatctgcag gcattaaaac agaatctgtc
     8401 cattgtccgc caggccgcgc cgcacgcgcg cgtctggtcg gtggtaaaag cgaacgctta
     8461 cgggcatggt attgagcgta tctggagcgc gctcggggcc accgatggct ttgcattact
     8521 taacctggaa gaggcaataa cgttacgtga gcgcggctgg aaggggccga tcctgatgct
     8581 ggaaggattt ttccatgctc aggatctgga gatttatgac cagcaccgcc tgaccacctg
     8641 cgtacacagc aactggcagc tcaaagcact gcaaaatgcg cggctaaaag caccgttgga
     8701 tatttatctt aaagtgaaca gtgggatgaa tcggttgggc ttccagcccg atcgcgtgct
     8761 taccgtctgg cagcagttgc gggcaatggc gaatgttggc gagatgaccc tgatgtcgca
     8821 ttttgccgaa gcggaacatc ctgatggaat ttccagcgcg atggcgcgta ttgagcaggc
     8881 ggcggaaggg ctggagtgtc ggcgttcgtt gtccaattcg gcggcgactc tgtggcaccc
     8941 ggaagcgcat tttgactggg ttcggcctgg cattattttg tatggcgctt cgccgtccgg
     9001 tcagtggcgt gatatcgcca ataccggatt acgtccggtg atgactctaa gcagtgagat
     9061 tattggtgtc cagacgctaa aagcgggtga gcgtgtgggc tacggcggtc gctatactgc
     9121 gcgcgatgaa cagcgaatcg gcattgtcgc cgcagggtac gccgacggtt atccgcgcca
     9181 cgcgcctacc ggtacccctg ttttagtgga cggcgtgcgc accatgacgg tggggaccgt
     9241 ctcgatggat atgctggcgg tcgatttaac gccttgcccg caggcgggta ttggtacgcc
     9301 ggttgagctg tggggcaagg agatcaaaat tgatgatgtc gccgccgctg ccggaacggt
     9361 gggctatgag ttgatgtgcg cgctggcgtt acgcgtcccg gttgtgacag tgtaacttgt
     9421 tgtaagccgg atcggaggca gcgtcttctg gttgcgaaaa aatcatccat ccggctggtc
     9481 agcaactgta gttgttaatg tgacagagcc attgcccatg atagtgtcca ttaaaaggat
     9541 ggacactatt tccccggaac ctgaactcac cgcacaggcg ttctacataa aacgcttacg
     9601 cttcattgtt gactcggagt cgacttcaga taaatcacgc tttacccttg atggagcctg
     9661 tacatagatt tgtgtaattg cctgattttg atatgttcaa ttcaacatca aatgaaggtt
     9721 aaattatgga cgacaaacaa ttgcaggctc aggctgcgtt cagcaaagca tcgcaaccgg
     9781 cgatagatgc ttcattaaat ttaagattca gcttcctctt cagccacccg tacgccaatc
     9841 ttcaacactt cattatcttc tttctcggcc accgtccaga tcatcccggc aaactctacc
     9901 tggtcaccga caaccggtgc cgcgcctaac aactgctgga caatttcacc cagcgtttgc
     9961 tgcttatcac gatattctcg cccgtcttcc aggccatata tcagcgcaac atcagcatat
    10021 ttggcgctgg cttcgagaat gaagtcacca aagaagcgtt gatccagcgc gaccggcggc
    10081 gactggctga acagtttacc gagcgccgga agatcgcgtt cccgaccaat tacacacaac
    10141 acatcgcctt cgcgcagtcg ggtgctgccg gtaggatgaa gcaactggtt atcacgaaac
    10201 agtgccgcaa tacgcgtctc ttttggcata tgcaaatcgc gcagtgccgc gcccacgcac
    10261 catttatcgg cactcaattg ataaacaaac tgctcccacg gattttccgg atgaatatcc
    10321 aggccaacgc gtgacaccgg acgtcccact ggcggaacca ccactttggc ttttttagcc
    10381 gcccacgaga gtgatgttcc ctgcaagagc agtgaaacca gaaccacaaa gaaggcgaca
    10441 ttaaagaaca gacgtgcatt ctccagcccc gccatcatcg ggaacacggc caggatgatt
    10501 ggcaccgcgc cgcgtaatcc tacccagctg ataaacacgc gctcacgcag attgaaaccg
    10561 cggaagggga gcaatccagc aaataccgaa aggggacggg cgaagaatat catccatgcc
    10621 gacaaaatga gcgccggaat ggcaataggc agcagatcgc ttgggttaac caatagcccc
    10681 agcaccagga acatggcgat ttgcgccagc caggcgaggc cgtcgaaatt ttgcaggatg
    10741 ccgtagcggt tgcgaatcgg ccgattaccc agcagaaaac cgcacagata aacagccaga
    10801 ataccgctgc cttccagcgc cgtggttaaa gcaaaaatta aaataccgcc gcttaatgcc
    10861 agcaatggat ataatccggc aggcagggcg atgcgattaa tcatttgcag cagtaaataa
    10921 ccaccgccaa gtccgatgac aatcccgagg ccaaattgct gcagaatatc gaccacgaac
    10981 atccaactga cactgctttc atgttgctgg atcatcgcaa tcagggtaat cgtcagaaag
    11041 accgccattg gatcattact gccggattca atttccagcg tcgagccaac acgttcgtta
    11101 agccccttac cacccagcag agaaaagacc gctgcagcat cggtagagcc gacgatagcg
    11161 ccgattaata agccttcaat caaatcaaga ttaaacagcc acgccgccat catgccggtt
    11221 aaaccagagg tgataagcac gcccagcgtc gccagcgaca gtgccggtcc taacgccaca
    11281 cgaaaggaac tggcctgagt gcgcatcccg ccgtcgagca aaataatcgc cagtgccagg
    11341 ttacttacca tgtaggcgaa ggggtaatta tcaaacggga taccgccgac gccatcgact
    11401 cctgccagca tgccgatcgc cagaaaaata accagaatag gaatgccaag acgggaagaa
    11461 aatgaactaa gtaatatact gctggtgaca aggatggatc ctaagatgaa aaggctaatt
    11521 attgttgtgg catccaacgg tcggttactc ctgattacgc tgtctcttat ataaacccta
    11581 ccatattagc ggcagagaca gcgttttact tagccctgaa gcgtgatttt tttacatttt
    11641 aagaacagga tgaccgctaa tcgtcaactg agtgccttcc cgggtgttat tcagaatggc
    11701 atgtgcgccc aacggcagcg taaccgtgcg ttgttcatga ccaaaatcga ggccggtaat
    11761 gagtggaatc gacaggcggg aacgcaaaaa cgcgtacact gactccaggt tgtaacctgc
    11821 gtcataatca ttgggcgtgc tgccgctaaa gctaccgaga atgatcgcct tctgacgtgg
    11881 caaaattccc gcatgataga gctgcaacag catacgttcg acccggaaag ggtgctcgtt
    11941 aatatcttcc agcaccagaa taccgttctc aatttttggc atccacggtg taccaatcag
    12001 tgaaatcagc atcgcaagat tgcctcccca caacgtgcct tcggtctgac atgtcgggcc
    12061 ttccccttgc cattcaatgg tgaaggtttt attgcgtaac gccagccaga aatggtgctc
    12121 ggtaaaggcg ttcagttcat ccgcgccaaa gtttgctacc agcatggggc cgctaaaggt
    12181 gatgacattg ccttgcgcca gaagaccgca ctgaatggcg gtaaaatcgc tatgcccgca
    12241 aatgagcaac gggtcatgtt gttggcgagc caccagtgcc tgccagtcaa tatctgccag
    12301 taaacgactg gcaccgtaac cgccgcgtac agccagcacg atggtgttgg gggtagtcag
    12361 tctggcaagg gaattgagat cttccagacg ctccgtttcc gtacccgcaa aacgctcaca
    12421 acgacgggca ataacctcga cgttattgac ctgatgcccc tcgtcggtaa ggcgctggat
    12481 accacgcagc gcggcgtgct gtttaatgca gtaacccgat ggggcaatta agtgaaacag
    12541 agacatggca attccttgct gacaacagaa acgaaatgta tatcatgccg cttaggtgtg
    12601 ccgttgtcac ctcaacggcg attccaggct ataaggatag aagaagtgaa attgagatgg
    12661 tttgcctttt tgattgtgtt attagcgggt tgttcatcaa agcatgacta tacgaacccg
    12721 ccgtggaacg cgaaagttcc ggtgcaacgt gcgatgcagt ggatgccaat aagccagaaa
    12781 gccggtgcag cctggggcgt cgatccacaa ttgatcacgg cgattatcgc tatcgaatcg
    12841 ggtggtaatc cgaacgcggt gagtaaatcg aatgccattg gtttgatgca gttaaaagct
    12901 tcaacctccg gacgtgatgt ttatcgccgt atgggctgga gtggtgagcc gacgaccagc
    12961 gaactgaaaa atccagagcg taatatttca atgggggcgg cttacctgaa tattctggaa
    13021 accggcccgc tggcaggcat tgaagatccg aaggtactgc aatatgcgct ggtggtgtca
    13081 tacgctaacg gggcaggtgc gctgctacgg actttctcgt cagatcggaa aaaggcgatc
    13141 agcaaaatca acgatttgga tgctgacgag ttcctcgaac acgtagcgcg aaatcaccct
    13201 gcgccgcagg ctccgcgcta tatctacaaa cttgagcagg cactggacgc gatgtaaatc
    13261 agtcgcgtac tttgtccgct ttctcccggg cttcccgctc gagagagaaa ataatccgtt
    13321 gtaattgccg ctccaccgtc gggctgacgt taagaaaacg gaagctcaga cggggagtgg
    13381 tgatggtttc attcttgcca tcaatcactt tgcgctcgct gatggagatt aactgggcgt
    13441 caaagtgaaa aacaccccat tgccccatgt tgacttcaat ctgagcgaag cgcatgcctt
    13501 cttgtaattc ggcaggcttt gctgtttcca gtaatgcgcc catgccgcct aacgacaaat
    13561 catacaggcg gaaacgtaac gtactgttat ccgccagttt ggtctggcaa aaataaggcg
    13621 gatggagtgg ggcggagatg cggaaatatc ggcgtcgttg tacaaaccat aaggtgggag
    13681 gcggtacggt aataaatgcc ggaagctgca agtattcact ctgctgtagt tgttcaacag
    13741 taaactcgac tttcgcaccc tgagtttcgg cggtaatggt aatgtgctgc gcctttagca
    13801 cggcgatgtt gtcttcggct tgactgccga aatccagcac cagtttatcc ggggttattg
    13861 ccagtatttt gctgatcagc tgcccgccat tccaactgag ccgcaaagga attgcggctt
    13921 tgtgcaaatc gcgtaacacg cccaggacgg ctaacggatt ttgtttcagg aactgctcat
    13981 ggtaatgact cacgcggaaa aactcctgac tgacaaactg tctttggttt atcggtcaca
    14041 gttaacaaaa cttaatacaa atgtgtgaat attttttaca tgctgttctt aaatcagccg
    14101 acatacgccc agcatcgatc ccgcccttgc ctatacttag agcgttgatg taagcatttc
    14161 ttgcgtccga ttcatcgaaa cgagggcctg aacatgggaa ttattgcctg gattattttt
    14221 ggcctgatag ccggcattat cgccaagcta atcatgccgg ggcgtgatgg tggtggattt
    14281 ttcctgacct gtattctcgg gatagtcggt gcggtggtcg gcggctggct ggcgaccatg
    14341 tttggcattg gcggctccat cagtggtttt aatctgcaca gcttcctggt ggcggtggtg
    14401 ggagctattc tcgttctggg catattccgc ctcctgcgaa gagaataaga ttttcataag
    14461 gcggatagcg atacagatgc cgctatccgc tttcacatca gaacgtatac tcgacacctg
    14521 ctttacgggt gaaaaaaatc aatatagcac ttcgatatcc gataaagtcg gttcccggta
    14581 acaacaacag aatccttttt taattattgt ttcgttgttt ttggtgtgat tcagaaaata
    14641 aatattttag acagtagcca gtcaggcgtt aaaaatgggg cggatagtga ggcggttttg
    14701 gctgcgctta ttgaacattt agaattgatc aatccttctg gcagattaag ccaaaatacc
    14761 cgttcggcaa tgcttcagtt acgcgaagag gaatggtctg aattcttttt ttggttgcta
    14821 aattctttgg agtgtctgga ttatgtgatt attaatttaa ctccagaaag caaaaaaacg
    14881 ttgatgagtg aacaccgtaa caatattcag gtagcaattg atgctttata tagccaacgt
    14941 cggcgtaaat cgccgggtga tgaaagtgaa acacttaccc gtagaaatga cgctatcttc
    15001 ggcaaccacg tatggcaaac atttgcgcaa tacttcccgc caggacttga aaaaccgtct
    15061 gtgtagaaaa aaccatagct atggtgttat ctgccgacaa cgccatccgg agaagccgaa
    15121 tgacgttgtc ggagtaagcg ctggttaagg tgtgggttgt gcctctttgg ttgagggttg
    15181 cgtcgttgct gacttaacgg tcggacgcgt cgccggaaca ttgtcacacg gttgctcttt
    15241 cgggcagatc aaatccagca ttttcagcgt cacgccattg gtccagccaa agccatcctg
    15301 taatggatat tcgccaccgc cgccccccgt tccggtggtg ctgacatcat atttttccac
    15361 cagctttttc tcccggtcat aggtgtgctg aacattggtc aggaagtgcc agctaatgtc
    15421 catcgccacc tctttttgcc cgtagttttg taatccttct gtcgcgaccc actgtaacgg
    15481 tgcccagcca tttggcgcat cccattgttg cccacttttc accgacgtgg tgttcaggcc
    15541 gccgggttgc agcagatgtg ttttcgtcgc cgtcgccatt ttgttggcgc gatctttcgc
    15601 tgccgcattg acgtacagcg ggaacagggc ggccgcggtt aactgattgc gcactttatg
    15661 acttttcagg tcgtaatcgg cataccagcc ttgttgatcg ttccacaggt atttttcgat
    15721 ccctttttga cgggcatttg ccagcgtttc gtactggttt gccatcgcgt tatctccggc
    15781 agctttgctg gcgcgggcga ggattttttc cattttaaac atcaggctgt tcagatcgac
    15841 cggtacgatg ctggtggtgc gtaaggtatt taactgctgc gggttgtcca tccagcgcga
    15901 gctgaaatcc cagccagacg cagcggcaga gcgcaggtcg cggtaaattt cagtggcagg
    15961 tcgattcgga ttgcttttgg cggtggcaat atcttcctcc catgactctg gtcgtggcgt
    16021 atcgcgatcg tcccagtagc ggttgagaag ggtaccatcc tgaagtttta caacgcgttt
    16081 ttcctgttgt ccggcttgca ggttttcaac accgtccatc cagtaagcat attctttttg
    16141 catttgcggc aggtattgct tcaacgcggc atcgccttca tgctgcgcca gtaactctac
    16201 catcagggca aagaagggcg gttgcgagcg gcttaaatag taactgcggt tgccgttggg
    16261 aatatgaccg taagtgtcta tttcatgagc aaaattggcc accatatccg cgactttatc
    16321 ccagtgaccg ctttcggcaa gtcctaacat ggtgaagtaa ctgtcccagt aatatacctc
    16381 gcgaaaacgt ccgcccggca cgacataagg ttccggcagc ggtaacagag aatcccattt
    16441 ttcggtgttt tcggtagaac gcgttaatac cggccaaagt ccgtcaatat gttcgcgcag
    16501 tgactgcccc tctggcggaa catatttctc gccctctttc ggcagggtga aattgacgtt
    16561 aacgaaatgg cgcagatcaa atccgctctg gttttgctgc atccgataat cagcaaggat
    16621 catcagcgga tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg gaaaaagttt
    16681 ggcgttttgc acatcattaa acagcggccc taataaaata tcaggcggct gtggtgttac
    16741 cggtgtttct tctgcctgca ccgatagcgc agcgaaacac aaaaagatac aggctggaat
    16801 taacgccatt ttttgcgggc gagaaggtgc gggggatttc atcaatcatt ctcctttggc
    16861 gaaaccgaat aaaacggtat tcaagtatca gaaaacctta gttcaggatc gcactagctg
    16921 catgatcgaa atctcattct gcgaactgtc aggcatttta gaaaagggaa ttaaagggat
    16981 aaataacggt gaaatagagt gatcaaataa cgtctgttta ttagccagtc agcgacgttg
    17041 ggttcgcgat tagcgaagaa aaatcatcat aaattggtaa aaaatataac aggtagttaa
    17101 aaaacatcag tgctgggtaa attgccggat gacatcagaa cgatgccatc cgaacggtgg
    17161 cttaaccctg acggttgaaa cgttgcgttt taacgtccag cgttagcgtt tcttctggtt
    17221 gtatcgcata cagtttctca ccctgctggc aaatccagcc aatccccagt tcacgggcga
    17281 ttagggcgct gtgggatacc ggacttccgg cgctaaggca gatacctttt acaaccgccg
    17341 gatccagttg cagtactgtg gaaggataaa tgttctccgc cagtagaata gttggcgagt
    17401 taaactgcgg gagttcttct ttcgtttggg tcaggtggac cagggtgcga tgcagaagat
    17461 cgtccacatc aatatagcga gcttgtagat attcatcatc cagttgctgg tattgctggc
    17521 taagttcttt aagaacttgc tgccaggcat attctgccgt gcaatgttca tgctgaagga
    17581 gttcgcttgc cgccgccagc agttccggat catctaacag tgtatggtga ccagaaaaga
    17641 ttgcggcaat atcgtcaagc ccgctggctt ctgcttttgc tgttaacgtc atcagatcta
    17701 ataacgtgaa gtcaatagcc tggcgtaatc gatcttgttc ttcttccacg gtcagggttg
    17761 attttgcctg taccgtacat aaaactggtt gataataaaa ggctttaccc gaaacaggcg
    17821 gaacgggacg cagagtaggt ggagcgactt cctccgtttc accaaagtta tcttcagcca
    17881 gctgacggaa agcgatcagt gcctcttcag cttctggccc tttcgcaatc aggcgcagcg
    17941 tatcgttata gcgaacttgt agtaacgcaa tctggttaat actctctggt gtgacgcatt
    18001 tgccgttttt ttccagcaac atatcggcat taaatgtcga taaggtataa accagccggg
    18061 aggccggacg tacatgcagg ccgttacggt tttttatgac caccgccaga gaacgggctt
    18121 cttcatcgta cgcaggacat gtgtcagaga ttttagtgtc ggaggacggt aaacccagtt
    18181 gttcacgttt ggcttccagc gcatgcatag cgtcaaagat aactttgtcg atatccgctc
    18241 ccgaggccgc gctgaccgtt gctgccagtg taccttcgac caacggcgca gcacacaaac
    18301 gtacttttgc ggcgatcttg ggagccagca attccagcgc agtttcagca ctcaataatg
    18361 cgctacccat atccatcatg accagcacat ggtcggcatc agcaacagat tcgatggcct
    18421 ccatcacttt gacggcatcg gtaccaatag gattttgtgg atcgtcaatt cccgcggcaa
    18481 tggcgatttt acaactatca ctcattaaca tctgacgggc taattcaccg acaccttctc
    18541 ccagtcggct gctatgtgaa actatgacca ggtttaccat caccaattcc ttactctttt
    18601 gcggctaacg ccaacatttg catcataaac atcaccgagg tcgcgccggg atcctggtga
    18661 ccaatactgc gttcaccgag ataactggcg cggcctttgc gggcttgcat cgtaatcgta
    18721 ctttgtgcag cggattcggc gattctactg gcagcttcga gcgccaccgg aacagagaga
    18781 ttttgctcgc tggactgacg taacgattcc accaccggca cccacacatc acacatggtt
    18841 ttatcgccag gttcggcttt cccgcgactg attacgccgt ccacgccatc gcggaacatc
    18901 tgataaagct cttccagtgt caggctttgc cgtgcctggg tcgcctgtgc ggcgcggata
    18961 aagaaggtac cgaacagcgg accactggca ccaccgacgc tggaaagcag cgtcataccg
    19021 gtattcttga gaatgaaacc gatatcttta tctgcgatag cagggagttt ttccaccact
    19081 ttgctaaagc ctcggttcat atttagcccg tggtcagcgt cgccaatttc gcgatccagt
    19141 ccggtaagat actcgctctc ggtgctgaaa atatcgccac aacgagtgag ccagttaaca
    19201 atttgagttc tgctcagtga cattgctttc tccttattta ccccagttaa gggccggggt
    19261 gtggaccggg gtgtcccaga gtgccagcgt ttcgtcatca actttcagta aggtgattga
    19321 gaaaccggtc atatccagtg aggtgcagta cgcgccaatt aaattacgtt cgatagtcaa
    19381 tcccgcttgc tggcaacgtg tggtcaggcg gttatagacg ccgtacagct cagaaagcgg
    19441 agttgcgcca agattgttaa ccagcgcaat cacccgatcg ccagactgga gcggttgttt
    19501 ggtttgttgt tcttcctgcc aactgccttg ttgataatcc cagaaacgca aagtgcgatg
    19561 gtatgagcca ttttccagca gggtgtcgaa catttcatcg acggtttgat caagggaaga
    19621 gaaggagcgg cggtcaatac ccggctcacc atgaatgccg acgccaaact ccatctcatt
    19681 atccgccagg gtaaaagaag gtttgcccgc ggcaggaacg gtacaggcac cgagagcgat
    19741 acctattgag tggccttgat tattcaactt acgccccagt tccgcacagg cgtccagtga
    19801 gtcgccacgc tccgccgctg cgcctacgag tttttcaatt aatacggtgt tggcaacgcc
    19861 gcgtcgcccg gcggtataaa gactgtcttt taccgcaacg tcgtcatcaa tgaccacagt
    19921 ggtcactttt acgccgctat cgtgcagtaa ctcggtcgct gtttcaaagt taagaatatc
    19981 gccggtgtaa tttttgataa tcaacagtac accttcgccg ccatcaattt gcatggagca
    20041 ttcaaagatt ttatcgggcg tcggtgaggt gaaaatttcg cccggacagg cccccgaaag
    20101 catcccctga ccgatataac cacagtgcat cggctcgtgt ccgctgccgc cacccgacag
    20161 cagggcgact tttcctgcaa caggggcatc agctcgggtg acatacaccg gatcctgatg
    20221 cagtgtcagc gatggatgcg ctttcgccag tcctgccagt tgttcgtcca gtacgtcttg
    20281 cacatcattg atcaattttt tcattatttt gctccagcaa ttacggtagg gcatggatga
    20341 tgttcaacga cacggcgacc actgactgcc gatgaatcca ttgtgcatca ggcaagggaa
    20401 aagaaaatta gcccaaatta tgtttcatag tgaaacatat gctttaatga atgttccata
    20461 ttgaaacttt tacgtgtatt aatacttaaa attgcgagcc ggaacacctt ttgtcataag
    20521 ggatgcggga tatgagtggc gcttttaaca acgatggtcg gggcatatct cccttaattg
    20581 caacctcctg ggagcgatgc aataagctga tgaaacggga gacatggaac gtaccacatc
    20641 aggcccaggg cgtgacattt gcttctattt atcggcgtaa gaaagcgatg ctgacgctcg
    20701 ggcaggctgc gctggaagat gcctgggaat atatgtcacc gcgagagtgt gcgctgttta
    20761 tcctcgatga aaccgcctgc attctcagcc gtaatggcga tccgcaaacc ttgcagcagc
    20821 taagtgcact gggattcaat gacggcacgt attgcgccga gggaattatt ggtacttgtg
    20881 cgctatcgtt agcggctatc tctggtcagg ccgtgaaaac gatggccgat caacatttca
    20941 aacaggcact ctggaactgg gccttttgtg caacgccgtt gtttgacagc aagggccgat
    21001 tgacgggaac aatagcgctg gcgtgtccgg ttgagcaaac taccgcagct gatttgccgt
    21061 tgacgttggc aatcgcccgc gaggtcggaa atttactgct gacggacagt ttgctcgctg
    21121 aaactaaccg tcatttaaat caacttaatg ccctgttaga aagtatggat gatggcgtga
    21181 ttagctggga cgtgcagggt aatttgcaat ttattaatgc ccaggcggcg cgggtcttgc
    21241 gccttgacgc gacggcaagt cagggaaggg caatcactga actcttaacg ttacccgccg
    21301 tattgcaaca agcaataaaa caggcacatc cgctcaaaca cgtagaagca acctttgaaa
    21361 gtcagcacca gtttattgat gcggtgataa cccttaaacc gataatagaa acgcagggaa
    21421 ccagttttat tttgttgctc catcctgtgg aacagatgcg gcagttgatg accagtcaat
    21481 taggaaaagt cagccatacc ttcgctcata tgccacagga cgatccgcaa acccgccgct
    21541 tgattcattt tggtcgccag gcggcgcgca gtagctttcc tgtcctgctt tgtggagaag
    21601 agggcgtggg caaggcactg ctaagtcagg caattcataa tgaaagcgag cgtgctgcag
    21661 gtccttatat cgccgtcaat tgtgagttat atggtgatgc ggcactggcg gaagaattta
    21721 ttggtggcga tcgcacggac aatgaaaatg gccgtctgag tcggctggaa ctggcgcacg
    21781 gcggcacgct gtttcttgaa aagattgaat atctggcggt ggagttacag tctgctttgc
    21841 ttcaggttat caagcagggt gttatcacgc gactggatgc gcggcgctta ataccaattg
    21901 atgtcaaagt gattgccaca acgaccgcgg acctcgcaat gctggtggaa caaaatcgtt
    21961 ttagtcgcca gctgtattac gcgttgcatg catttgaaat taccatcccg cctctgcgta
    22021 tgcggcgtgg cagcattccg gcgctggtga ataacaaatt acgcagtctt gaaaaacgct
    22081 tctctacgcg gctgaaaatt gatgacgatg ccctcgctcg cctggtttcc tgtgcatggc
    22141 ctggcaacga ttttgaactt tacagcgtca tcgagaatct tgctctgagt agtgataatg
    22201 ggcgcattcg cgtcagtgat ttgccggaac atctgtttac cgagcaggca acagatgatg
    22261 tcagcgccac acgcctttcc accagtctgt catttgcgga agttgaaaaa gaggcaatta
    22321 ttaacgcagc ccaggtcaca ggcggtcgca ttcaggaaat gtcggcttta cttgggatcg
    22381 gccgcactac gctgtggcgg aaaatgaagc aacatggcat tgatgcaggg cagtttaagc
    22441 gccgggtatg aaagacagag acgatttctg atacatcaga gtgatctata tttcattccg
    22501 gcgcacgcta acatttttca gcatcgttta agggctggtc tatcccgcac ttaaaagctg
    22561 aagcgatatc ctccgttgac ttgcttctga tcaaagaggt tactctgcgt gtaatccgct
    22621 tcgaggtaga atgtgtgctg tttgttatac tgtgcactga ctcccacgcc gttattccag
    22681 ccattacctt tgaaactgta cttctcccgg gagttgttca acagatagtc tgtatcccct
    22741 gaaaactcgc ggatagctcc agtcttcaca tacatattca gctggctgtt gcctgcggtg
    22801 atgtcatatc ccagtatcat gctggcacgc cccagcagcg attcgtagtg attcagatgt
    22861 atattgagac cattactcgc cttcatagcc atctcattct ggtggctgta tgtgagctgg
    22921 gtttgcggct ctatatagaa cccataacca gtaggggtca ggttgaacct ctgcccggct
    22981 tccagggaga tgctcaatcc attcgcagtg ccgttggcgt taacgccgtt gttctgactg
    23041 tccagtacgt gaaaactatt tttctggcgc gatgctttta caacgagatc gctgtaaaaa
    23101 ccgttgtgtg ccatgtaact ggcatacatt cccatgtagt ctgaacgtgc ggtaccgtcg
    23161 cctccgctat agtccggcga tgcatgtgtt gagccaatat acagaccgac atacaacggc
    23221 attacatcag agagacgttt atccccacca aactggatac cgctgtaacc catgtcaaag
    23281 ccgctcagtt tgccactggc gaaggagtcc aggcttcccc cataactgcg cagccagata
    23341 ttaccgtctt tactctgatt tcgcaggtca cccattcgtt gcatcagcgt acggttttca
    23401 acatagttta ataagtaacc gacattgaga taattgccgc cagcatctgc ggtcgtggtg
    23461 ggtttaggtg tgggagtggg gttaggcgta ggatcgggtt caggcgtagg atcggggttg
    23521 actatgggag gctgagcggg agccggtgtt ggttcaggat taggagtcgg ttccggaact
    23581 gtcccggaag cgtaaagctc ccagttagtg ccatttttac gcacatcgta cagatatccc
    23641 cccaactcaa cctgagaaga cgcgctgaac gaggccgcac cgtcagtggt ttttaccact
    23701 gtcagaactt catttcccgt agtggcctcg ctgccctggt tgcggatagc caatacgtga
    23761 ttaccagcac tacttccgct gatattcaat aaatcccctt tattattaac gccattaccc
    23821 tcgccaacaa catcagcacg cataataaag gtactgttac cgctcaggtt ctctacgttt
    23881 aatgtggcaa atgtgccggc agttgacccg tggctggcaa aatcgacagt tgaatggctc
    23941 agcgccagcg tgtcgagatt agaattactt gttacgttcc agacgctgtt attcattgca
    24001 acgtccagtt tcccgccatt gacattatcg ctgagggagg aacctgtcca aaccgaccct
    24061 gggtgcatat ctagattgat tagcccacct ttggaaagaa cgctaccgtt gataagcata
    24121 cgacccgagg cgttgatgcg gctggcggca taaccatcgt catgttgcgt tgcaatggcc
    24181 atctggtcgg gtgtactcat atcaatgacc agatcgctcg tgaggtcgat ctggctgttg
    24241 gtcatggcat aaattcccct ggctcctgcc gcgccggtga tagccaaact gtctccggtt
    24301 attctaccgc cgctgagcgc ccacaaaccc agcgccagac tgccattacg atcaactgta
    24361 atatcggtat tttgcatgtt gacaacagcc gttgccgtct gggccgaggc accataagaa
    24421 ccgccggaaa agatgctgtt tcgttgcgct gccgtgccag aaaaattgat tgtcgcgtct
    24481 gaaccactgg tgacgaggcc accgccctgc gcggaagaaa tatggctatc tgcaccgata
    24541 gtggttgtac caccacgaac ttcgacgcca ttggccgcgg ctccagttac atcaacagtg
    24601 agtgcattcg cgctaacctg gccaaagctc cagaggccgt gtgcattatc gccattggtt
    24661 ttaatggtac tgtttgttcc gagatcgaca acagagtttt tctgtacgtt tatccccatg
    24721 gcgctgtagc cctgaacatc gattgtcagg tctgtcgccg taaaacgcgc agcaccattg
    24781 gcgttattgc cgttgagacc accgatataa acacctgtac ttccatcggt cgtgatttta
    24841 cttccgcttc caagatcgac actggtgcca tagtcattga tggttaggcc tataccgttc
    24901 gagttttcaa tggtgaattg agtggctgtt agtgttgagc tatgcccaat aatgatgccg
    24961 tcatcgttac tcttaatggt gctgcctgtg cctaagtcag catgggtata gtcaccaatt
    25021 aagttaatgc cgatggcaga ggtttgacca acaacatcta ctgtgagtcg gttagctgtc
    25081 agggagctgt ttttgccctg aataatgatt ccttttgcat atccagaggc gtcgttgaca
    25141 ttgacggtga catcattacc caggttgata ttgccaggcg tgtttcctgc tggggtcatt
    25201 acaccataga ggtttcctga tggatcggcg gtatctcctg taattctgtc accatcatta
    25261 atagtaatat tggtgccata ttgacttcct gtatcagcag ctatcagcag gggggaaaag
    25321 ataattgcat tcaaaccaat agcgccaaat tttatcagtt gaattgaggg cgaacggatt
    25381 tttaattcag cgagacgatc tgctttggta ttcccattgt gttgtttgat gcccatacca
    25441 ctcctatata gtacccagac tatgggtcta tggattttgt cattttgttt aaatcaatga
    25501 ataatatcct cttatcatca tgatatttgt atggtgcaat catgatgagc ttctcagaga
    25561 ataattctct attaccgggc aaaatcagga acacaggaat taatatatat ttaacatccc
    25621 ccttacaaag gtgggtttat atcgagttat tccctaagtt gtcgttgctt attccaattc
    25681 gttactataa atatggcaaa aatattacaa caaacagcgg ttaaaaaatt tggtgaagat
    25741 tttttgtaat aatcctaatt agttatggga atttcgcttg atgtatctgc ataacaaaaa
    25801 caatatgcgc catttttgca ttactaataa gaaaaagcaa aaaacatcga tattcatttt
    25861 tggtcaatgg gttttctggc ctatcgttgt tattgtgcta ctggttatca ttttcatctt
    25921 gccaggatcg cacgtaatca cgttattcgg ggactttatc tgcgggatat tacccttcag
    25981 gtaagtcctt ttctcgccag catattggag cgtgatcaat attgatcagc tgtgaagggg
    26041 gtgatgtgta gtggaccaaa acttacgtta tatcactact aatgagatat ccgtcatctg
    26101 acactgatgt ctttaattat tctcgtggtt cactgtaggc aataagcaca aaagtgtagg
    26161 atgttacaag aatgattagg actcggtgaa atgaaaaatc cacgcaattg cgtggattat
    26221 atatactttt gcgctcttca tgagatttag cgaaacctca tgagacaaca aattaattag
    26281 acgttgaaaa ggaagttcat cacatcgcca tctttaacga tgtaatcttt accttctgca
    26341 cgcattttgc ctgcttcttt cgcgccttgt tcacctttgt aagtgatgaa atcttcaaac
    26401 gagatggttt gtgcacggat aaagcctttt tcaaaatcag tatggatttt gcccgctgcc
    26461 tgcggcgcgg ttgctccaac cggaatggtc catgcacgca cttctttcac cccagcggtg
    26521 aagtaagttt gcaggttcag cagcttataa ccggcacgga tcacacggtt caggcccggc
    26581 tcttccagcc caagctcctg cataaactcg tcacgttctt cgtcgtccag ttcggcaatg
    26641 tctgcttcaa cagcagcaca aaccggaacc acaacagaac cttctttcgc cgcgatttca
    26701 cgcacctggt caagatatgg gttgttttca aaaccgtctt cgttgacgtt ggcgatgtac
    26761 attgttggtt tcagcgtcag gaagctcagg taacgaatag ccgctttctc ttcagcgctt
    26821 aaatccagcg cgcgcagcat acctgcattt tccaactggg gcaggcattt ttccaggacc
    26881 gccagctcag ctttcgcgtc tttatcgcca cctttggctt tcttctgtac gcgatgaatc
    26941 gcacgttcgc aggtgtcgag gtctgccagc gccagttcgg tgttgataac ttcaatatcg
    27001 tcagccgggt taactttgcc ggaaacgtga atgatgttgt cattttcaaa gcagcgaaca
    27061 acgtgaccga tcgcttcggt ttcacggatg ttggtcagga actggttacc cagaccttcg
    27121 cctttcgatg cgccttttac cagaccggcg atatcgacaa attccatggt cgtgggaagc
    27181 gtacgttgcg gttttacgat ttcagccagt tgatccaggc gagggtcagg cattggtacg
    27241 acgcctgtgt tcggctcaat ggtgcagaat ggaaagttgg ccgcttcaat accggctttg
    27301 gtcagcgcgt tgaacagggt agatttcccg acgttgggca aaccgacgat accgcatttg
    27361 aatcccatga tttaaatcac cttaatatct taataatcaa cctgttattg ctaacagatt
    27421 gcagaaatgg aaataacttt gcctattata cacggcactc ggcaaaaatg ccgcagacaa
    27481 cgacttattg cgctttaaag gcgtgcaatc ggttcgttgc tttggtcaag ccatctgtaa
    27541 accacatttc agtacaacgc gccgcttcgt cgatggcttc atcaattaac ttctgttcac
    27601 taacaggcgg tttgcctaac acaaaaccga caactttatt tttatcgccc ggatgaccga
    27661 ttccgatgcg taaacggtga aagttagggt tattacccaa tttactgatg atgtctttca
    27721 gtccattgtg accaccatgg ccaccgccca atttaaattt ggcgacgcca ggaggcagat
    27781 ccagttcgtc gtgggccacc agaatttcgt ccggattaat gcggaaaaaa ctggccatcg
    27841 ccgcaacggc tttgccgctg agattcataa atgtagtcgg gactaacagg cggacatctt
    27901 cgcctccaag agtgactcgc gaagtataac caaagaattt agcctcttcg cgcagcggag
    27961 cgcgcaaacg ctctgccagt aagtcaacga accaggcacc agcattatgt cgcgttgcgg
    28021 cgtattcagc accggggttc gccaggccga caatcaattt aatcgtcacg tttttttgtc
    28081 ctgagtgtgt acataactgg cgcgtagttt actggttgcg gccccgcttg acaaaaaact
    28141 gcgtatcaaa tgcagataac gtaataattg catgagtgga ctattagaaa gtcaaggtgt
    28201 tcaggcgttt atttgtaaag ttttgttgaa ataagggttg taattgtgat cacgcccgca
    28261 cataacccac agggtgttgt ctatacttta cacataagga agaggggtat tccctgttac
    28321 aacccagaaa gttccggagg tgacatatga aacgcaaaaa cgcttcgtta ctcggtaacg
    28381 tgctcatggg gttgggtctg gtggtaatgg tggtcggcgt ggggtattca atcctcaacc
    28441 agttaccaca gtttaatatg ccccagtatt tcgcacatgg tgcagtgcta agtattttcg
    28501 tcggtgccat tctctggctg gcgggtgccc gtgttggcgg gcatgaacag gtgtgcgacc
    28561 gttactggtg ggttcgccac tatgacaaac gttgccgccg tagcgataat cgccgtcata
    28621 gctaacaaat aatgcagttt gctgaccagt cagtttgcgc tgactggtca atctcgtact
    28681 tataaatccg ccatcgccgc gcgacgattc gggaagaacg ccaggcgtcc cgggatcggt
    28741 tgaatgccag cgcgagccat agtgcgcagt ggctggaatt ccacgttgca cacgcgcagt
    28801 tcacatccct cgggcagacg cttcacaaaa cgctggaacg catcaagacc accagcatca
    28861 agtaccggaa ccgcatccca cttcagaatc acaatccgtt tgccttcaag acgtgactcc
    28921 aggtccgtga acaagccttc agcagcagca aaaaacagcg ggccaataac gcgcagaacc
    28981 aggacatcgt ctggaacatc tacgactacc ggtgccaggc gagtcatacg tgcgatacga
    29041 cgcataaaca gcagcgatgc cagcacgatc cccacgctga tggcaataac catatcaaac
    29101 agcacggtca gcgacatgca cagcagcatg acaatgatgt catctttcgg cgcatgacgc
    29161 agcaagtcga ccactttgtg cgcttcactc atgttccacg ccaccatcaa cagcagggca
    29221 gccatggcgg aaagcggcag ccaggagagc agcggtgcca gtaccagcag ggcaagaata
    29281 accagaatag agtggatcac cgccgagata ggggaagttg ccccggcacg gacgttagcg
    29341 gcagaacgcg cgatggcggc tgtagcggta atgccaccaa agaacggagc gatgatattc
    29401 cccagtccct gtccaaccag ttcgctattc gccttgtgtt tcgtcccggt cataccatcc
    29461 agcaccacgg cgcagagcag agattcgatt gcgccgagca ttgccattga gaatgccgca
    29521 ggcagcagtg tgcgaataga atcccaggtt agcgtgaatt ctgaattagg cagatcccac
    29581 ggcagcacca gttgcggcag cagttgcgga ataccgttac cctgagaacc atcggccagg
    29641 acgtagtgga attgcgaacc gatggtagca acatgtccgc cgagcaggtt aacaatcccc
    29701 atcaccgcgc aaccagccag caaggccgga aggtgaccgg gtaaacgaat gcccagacgc
    29761 ggccaaaaaa caagaatacc tagcgtcaca atgccaatgg cagcatcacc cacattaatg
    29821 gtcggcagcg ccataaataa tgcgccgact ttttgtagat aatgttccgg gacatgggcc
    29881 atttgcagac cgagaaaatc tttaatctgc atggtaccga tggtgatccc gatacccgag
    29941 gtgaaaccta aggtgacgga aaccggaata tactcaatca ggcgaccaaa gcgtgccaga
    30001 cccatcagaa tcaaaaagat ccccgacagc aaggtcgcaa ccagcagtcc tgccagtcca
    30061 aactgttgcg acacgggata gagaattacc acaaatgccg cagtcggacc ggaaacgcta
    30121 aagcgtgacc cacccgtcag agcaatgaca atccccgcaa cagctgcggt atataaaccg
    30181 tactggggtg ccacaccact accaatagcc aacgccatcg ccagcgggat agcaataatc
    30241 ccgacggtta tcccggcaat caggtcacgg gtaaaccgtg cggcagtata tttttctttc
    30301 cagcaagcgt cgatcagagc gcggaaaggc atcacatgtg aggaaaatat tttgttcaca
    30361 ataatgtttc atccgtgagc gcatcatctg tcaactaaat ggcaggtgaa ggaggcatag
    30421 gtcatacaaa tggatattac agacaaaaaa acccgccgca gcgggtcttt gagccgggtt
    30481 cgattagtgt tcgaacatgg cagagatcga ttcttcgttg ctgatacgac gaatcgcttc
    30541 ggccagcata cctgacaggg tcagagtacg cacgttcggc agtgatttga tttcatcgct
    30601 cagcggaatg gtatcgcaga caacgacttc atcaattaca gagttacgca ggttgttcgc
    30661 cgcgttgcca gagaagatcg ggtgagtcgc gtacgcaaat acacgtttag caccacgttc
    30721 tttcagagct tcagcagctt tacacagcgt accgccagtg tcgatcatat catcgaccag
    30781 tacgcagtca cgacctgcaa cgtcaccgat gatatgcatc acctgggaaa cgttcgcacg
    30841 cggacgacgt ttgtcgatga ttgccatatc ggtatcgttc agcagcttag cgatagcgcg
    30901 ggcacgcaca acgccgccga tgtccggaga aaccacaatt gggttatcca gattcagctg
    30961 cagcatgtct tccagcagga tcgggctacc aaatacgtta tcaaccggaa cgtcgaagaa
    31021 accctgaatc tgttcagcgt gcagatccac tgtcagcaca cggtcaacac cgacgctgga
    31081 gaggaagtct gcaaccactt tcgcagtgat tggtacacga gcggaacgga cgcgacggtc
    31141 ctggcgcgca tagccaaagt aggggataac agcggtgata cgacctgcgg aagcacgacg
    31201 cagggcatca accataacga ctaattccat caggttgtcg ttagtagggg cacaagtgga
    31261 ctggatgatg aaaatatcac caccgcgtac attttcatta atttgtacgc tgacttcgcc
    31321 atcgctaaag cgacctacag cggcgtcgcc gagtgaagtg tacaggcggt tggcaatacg
    31381 ttgtgctagt tccggggtgg cgttaccagc aaaaagcttc atatcaggca cgagaagaac
    31441 ctcaggcatg cgtccattgg tggaaagaat ctgccgaaaa ctgtgcgggc caggcatgat
    31501 cctttccagg cggtgtatta aagagcgcga tgcaacgtct ggaacaaggt gacgttgtca
    31561 ccgaaactca gcttgcccgg cttaaagcat ggctctgtgc aatggggaaa gattaacgcc
    31621 tttcgccaca aagccattga gccattccgg ggcttgctct agcacctggc gggcttcaga
    31681 ctctgtatca aattcagcaa agacacaggc ccctgtccca gtcaggcgcg acggggcgta
    31741 ttctaacagc caggaaagca ccgcatcaac ctcgcgaaaa cgttttcttg cgataacctc
    31801 gcaatcattg ctgaattcac attttagcaa cgtttctatt gacctttttg gcgtattgcg
    31861 cgggagttca ggatctttaa aaatcaccgg agtcggaata cttacaccag ggtgcgccac
    31921 cagataccac ttctctggcg gatccaccgg cgttagtatt tcaccaacgc cttcggcaaa
    31981 cgcggcatgc ccccgaacaa agacaggaac atctgcgccc agcgtcagcc ccatttccgc
    32041 cagctcatcc atgcttagcc cgcattgcca gagatgattt aatgccacca ggaccgtcgc
    32101 ggcattggat gaaccaccgc cgagaccgcc gcccatcggc aaacgcttgt caatgctgat
    32161 attcgcaccg cttcccgtcg gaagacgccc gctgtctgcc gcagttttca tcaacaaccg
    32221 cgctgcgcga acgatcaggt tatcttcatg ttccacgcct tcaacgggcg ttaacagacg
    32281 aatatcccca tcgtcacgaa gctcaatgct gatggtgtcg ccgtaatcaa gaaactgaaa
    32341 cagcgtttgc agcgtgtggt aaccatccgc acgctgaccg gtaatgtata aaaacagatt
    32401 aagttttgcc ggagagggcc actgtgtccg cattatttca ctatccagtt atccattttt
    32461 aacttgatgc gttgaccacc gtcggtgagt tccatattgg ctggcatcgc aggttgcgtt
    32521 ttggtgtcat aaccaccata aacaaccttc cagtttttgc cattctggct gtaggtaatt
    32581 tcgctcaggc ggtactggtc gtccagtttg tagtcggttg catcacccgg taaacctaaa
    32641 atccactggc gcaagctgtt gagcggaatt ggcattccgg tcaatttgcc aatcatctct
    32701 tcggcgtcat cggcggtata acgctgacct ttattgtcga ctaactgcac gttacccggt
    32761 tgagcattca gctccagttc cgtgctgccc aatgggttag tgagcagcag acggtagcga
    32821 tcctggccgg tttgctgcca gaaaaagcgg gcgtacactt tttgttggtc agaaatataa
    32881 gcgaacgcgc cgcgagtctg atactgatta agattgcgca cgtcttgctg atgctgacgc
    32941 cattgtggcg aatccgggct tttgccagga cctttgggcg tggtaacgga acaggcagta
    33001 agcacaagag cagccagcgg tagcaggcgg ataagacgaa aatcgggcag gggcatagtg
    33061 atgacaagtc cttgagatac gttgcagtta taacccttaa tgctagcgtt accgtccgct
    33121 atcgtctatg ttcaagttgt cttaattgcc agaatctaac ggctttcggc aattactcca
    33181 aaagggggcg ctctctttta ttgatcttac gcatcctgta tgatgcaagc agactaaccc
    33241 tatcaacgtt ggtattattt cccgcagaca tgaccctttt agcactcggt atcaaccata
    33301 aaacggcacc tgtatcgctg cgagaacgtg tatcgttttc gccggataag ctcgatcagg
    33361 cgcttgacag cctgcttgcg cagccgatgg tgcagggcgg cgtggtgctg tcgacgtgca
    33421 accgcacgga actttatctt agcgttgaag agcaggataa cctgcaagag gcgttaatcc
    33481 gctggctttg cgattatcac aatcttaatg aagaagatct gcgtaaaagc ctctactggc
    33541 atcaggataa cgacgcggtt agccatttaa tgcgtgttgc cagcggcctg gattcactgg
    33601 ttcttgggga gccgcagatc ctcggtcagg ttaaaaaagc gtttgccgat tcgcaaaaag
    33661 gtcatatgaa ggccagcgaa ctggaacgca tgttccagaa atctttctct gtcgcgaaac
    33721 gcgttcgcac tgaaacagat atcggtgcca gtgctgtgtc tgtcgctttt gcggcttgta
    33781 cgctggcgcg gcagatcttt gaatcgctct ctacggtcac agtgttgctg gtaggcgcgg
    33841 gcgaaaccat cgagctggta gcgcgtcatc tgcgcgaaca taaagtacag aagatgatta
    33901 tcgccaaccg cactcgcgaa cgtgcccaaa ttctggcaga tgaagttggc gcggaagtga
    33961 ttgccctgag tgagatcgac gaacgtctgc gcgaagccga tatcatcatc agttccaccg
    34021 ccagcccgtt accgattatc gggaaaggca tggtggagcg cgcattaaaa agccgtcgca
    34081 accaaccaat gctgttggtg gatattgccg ttccgcgcga tgttgagccg gaagttggca
    34141 aactggcgaa tgcttatctt tatagtgtgg acgatctgca aagcatcatt tcgcacaacc
    34201 tggcgcagcg taaagccgca gcggttgagg cggaaactat tgtcgctcag gaaaccagcg
    34261 aatttatggc gtggctgcga gcacaaagcg ccagcgaaac cattcgcgag tatcgcagcc
    34321 aggcagagca cgttcgcgat gagttaaccg ccaaagcgtt agcggccctt gagcagggcg
    34381 gcgacgcgca agccattatg caggatctgg catggaaact gactaaccgc ttgatccatg
    34441 cgccaacgaa atcacttcaa caggccgccc gtgacgggga taacgaacgc ctgaatattc
    34501 tgcgcgacag cctcgggctg gagtagcagt acatcatttt ctttttttac agggtgcatt
    34561 tacgcctatg aagccttcta tcgttgccaa actggaagcc ctgcatgaac gccatgaaga
    34621 agttcaggcg ttgctgggtg acgcgcaaac tatcgccgac caggaacgtt ttcgcgcatt
    34681 atcacgcgaa tatgcgcagt taagtgatgt ttcgcgctgt tttaccgact ggcaacaggt
    34741 tcaggaagat atcgaaaccg cacagatgat gctcgatgat cctgaaatgc gtgagatggc
    34801 gcaggatgaa ctgcgcgaag ctaaagaaaa aagcgagcaa ctggaacagc aattacaggt
    34861 tctgttactg ccaaaagatc ctgatgacga acgtaacgcc ttcctcgaag tccgagccgg
    34921 aaccggcggc gacgaagcgg cgctgttcgc aggcgatctg ttccgtatgt acagccgtta
    34981 cgccgaagcc cgccgctggc gggtagaaat catgagcgcc agcgagggtg aacatggtgg
    35041 ttataaagag atcatcgcca aaattagcgg tgatggtgtg tatggtcgtc tgaaatttga
    35101 atctggcggt catcgcgtgc agcgtgttcc tgctacggaa tcgcagggtc gtattcatac
    35161 ttctgcttgt accgttgcgg taatgccaga actgcctgac gcagaactgc cggacatcaa
    35221 cccagcagat ttacgcattg atactttccg ctcgtcaggg gcgggtggtc agcacgttaa
    35281 caccaccgat tcggcaattc gtattactca cttgccgacc gggattgttg ttgaatgtca
    35341 ggatgaacgt tcacaacata aaaacaaagc gaaggcgctt tcagtgctcg gtgctcgcat
    35401 ccacgctgct gaaatggcaa aacgccaaca ggccgaagcg tctacccgtc gtaacctgct
    35461 ggggagtggc gatcgcagcg accgtaaccg tacttacaat ttcccgcagg ggcgcgttac
    35521 cgatcaccgc atcaacctga cgctctaccg cctggatgaa gtgatggaag gtaagctgga
    35581 tatgctgatt gaaccgatta tccaggaaca tcaggccgac caactggcgg cgttgtccga
    35641 gcaggaataa tggaatatca acactggtta cgtgaagcaa taagccaact tcaggcgagc
    35701 gaaagcccac gccgtgacgc tgaaatctta ttggagtatg tcactggcag ggggcggact
    35761 tttattctgg cctttggtga aacgcagctg actgacgaac aatgtcagca acttgatgcg
    35821 ctactgacac gtcgtcgcga tggtgaaccc attgctcatt taaccggggt gcgagaattc
    35881 tggtcgttgc cgttatttgt ttcgccagcg accttaattc cgcgcccgga tacggagtgt
    35941 ctggtggagc aggcactggc gcggttgcct gaacaacctt gccgtattct cgatctcggg
    36001 acgggtaccg gggcgattgc gcttgcgctg gctagcgagc gcccggactg cgaaattatc
    36061 gctgtagatc gtatgcctga tgctgtctcc ctggcacaac gtaatgccca gcatctggcg
    36121 atcaaaaata tccacattct gcaaagcgac tggtttagcg cgctagccgg gcagcagttt
    36181 gcgatgattg tcagcaatcc gccgtatatt gacgagcagg acccacatct tcaacaaggc
    36241 gatgtccgct ttgagccgct cactgcgctg gttgcggcag acagtggaat ggcagacatc
    36301 gtgcatatca tcgaacagtc gcgtaacgcg ctggtatccg gcggctttct gcttctggaa
    36361 catggctggc agcagggcga agcggtgcga caagcattta tcctcgcggg gtatcatgac
    36421 gtcgaaacct gccgtgacta tggtgataac gagcgcgtaa cgctcggccg ctattatcaa
    36481 tgacaagttt ttctacactg cttagtgttc atcttattag tatcgcgctt tctgttgggc
    36541 tattaacctt acgtttctgg ctacgttatc agaagcatcc acaggcattt gctcgctgga
    36601 cgcgcattgt gccgccggtt gtcgatacgg tgttattgtt aagcggcatt gcgttgatgg
    36661 ctaaagcgca catcctgcca ttttccgggc aggcacagtg gctgactgaa aagctgtttg
    36721 gagttatcat ttatatcgtt ttgggtttta ttgcactcga ttatcgtcgt atgcacagtc
    36781 agcaggcgcg cattattgcc ttcccgctgg cgttggtggt gctgtacatc atcattaaac
    36841 tcgccaccac aaaagtaccg ttactggggt aagtcatgag atcgttagct gatttcgaat
    36901 ttaataaagc gccattgtgc gaaggcatga tcctggcttg cgaagcaatc cgccgcgatt
    36961 ttccctcgca agatgtttac gacgaactgg agcgtctcgt tagtctggcg aaggaagaaa
    37021 tcagccagct tctgccttta gaagagcagc tggaaaaact gatcgcgctg ttttacggcg
    37081 actggggatt taaagcctca cgcggcgttt atcgtctttc cgatgcatta tggctggacc
    37141 aggtgttaaa gaatcgacag ggcagtgcgg tatcattagg tgcggtttta ttatgggtcg
    37201 cgaatcgtct cgatttgccg ctgctgccgg tgattttccc tacgcagctg atattgcgca
    37261 ttgaatgtcc ggatggcgaa atttggctga ttaatccttt taacggtgaa tcgttaagcg
    37321 aacatatgct ggacgtatgg ttaaagggaa atatcagccc gtcggcggaa ctgttttatg
    37381 aagaccttga tgaagctgat aacattgagg taatccgcaa attgctggat acactcaaag
    37441 cctcgttgat ggaagaaaat cagatggagc tggcgttacg caccagcgaa gctttattac
    37501 aattcaaccc tgaagatccc tatgaaattc gcgatcgcgg gttgatttat gcgcaactgg
    37561 attgcgaaca cgttgcgttg aacgatttaa gttatttcgt tgaacagtgt ccggaagacc
    37621 cgatcagcga aatgatccgt gcgcaaataa ataacatcgc gcataaacat attgtgctgc
    37681 attaattaat cgacatttta ctcaagatta aggcgatcct atgaaacaaa aagtggttag
    37741 cattggcgac atcaacgtag caaatgacct gccgttcgta ctgtttggcg gtatgaacgt
    37801 gctggaatct cgcgatctgg cgatgcgcat ttgcgagcac tacgtaactg tgacccagaa
    37861 actgggtatc ccttacgtgt tcaaagcctc ttttgacaaa gccaaccgct cctccatcca
    37921 ctcttatcgt ggaccgggcc tggaagaagg gatgaaaatc ttccaggagt tgaagcagac
    37981 ttttggcgtg aaaattatca ccgacgttca cgaaccaagt caggcacagc ccgttgctga
    38041 tgtcgtggat gtgattcagt tgccggcgtt tcttgctcgc cagactgacc tggttgaagc
    38101 catggcgaaa accggtgcgg taattaacgt caagaaacca cagtttgtca gcccgggaca
    38161 gatgggtaat atcgttgata aattcaaaga aggcggcaac gaaaaagtga ttctttgcga
    38221 tcgtggtgct aacttcggct atgacaacct ggttgtcgat atgctgggct tcagcattat
    38281 gaagaaagtg tctggtaact cgccggtgat tttcgacgtg acccacgcac tgcaatgccg
    38341 cgatccgttt ggcgcagctt ccggtggtcg tcgtgctcag gtggctgagc tggcacgagc
    38401 cggtatggcg gtaggtctgg cggggctgtt tattgaagcg catccggatc cggaacatgc
    38461 gaaatgtgat ggtccatccg cgctgccgct ggctaaactg gaaccgttcc tcaagcagat
    38521 gaaagcgatt gatgatctgg tgaaaggttt cgaagaactg gataccagca agtaatcttt
    38581 tttgcttgaa aaataaagtc ttagcgttct gcgttaagac ttttttcatg ggtgccagat
    38641 acaaaaaagg ccgcaggctg ttacccctgc ggccggtttc gggcgcatat tgccatcacg
    38701 gcagcctgac gcccgttttc accttacttc cggttacgcc accagccgac aatcgctgcg
    38761 gtaataattc ccgccaggat cggtgctgcc aggtcgtgcc agaaaatcat ggcaaactgc
    38821 gcgagcgtca tatagccgcc ttgttgtaat gacaacattt tgcggctatt cttgaattgt
    38881 tctggttcaa gattagcccc cgttctgttg tcaggttaaa cctctcaacg tgcgggggtt
    38941 ttctctttcc agcaaccaat gccaccaggg ataaagcccc cgcaacattg cgcctcaccg
    39001 gataacgccg gcttgatgtg gatactacgt ctcaattcat cttcacttca tccctgaaat
    39061 gtttgcaatg aagagtgcat tccggttttt caacagctgt tacagtcgtt tcatgagggc
    39121 tctggatgat gcttccagct cgggttgtat agcgcggcga tttttttgat accgatttgc
    39181 atttcatttg ataccgctga aaaacgccga tacgcaactt atttgatact aaatcggcgc
    39241 ttttcttttg agttttagaa tgagttttta agcgctcatt cactctcttt caaaccagtt
    39301 ttaactgttt gtggttttgg gtaaggctgg actttgaata gctttgtgtt gaggcgtttt
    39361 ctggccctct tgttcagaaa ccggatgtaa cgaaactgtc ggaacttatg cacactggct
    39421 ctgtcgatat tagccctcag atgttcacct cgttgtccgc ctcgtttaat ggcactcctg
    39481 caaatctcgt gataccactc gccatcaagt tcatagaatg ttgattcatg gctgcctaca
    39541 taatcaaaat tgcttgcctg atacacgaca ccaagacagc cacaacgctc atctgcaaac
    39601 gactgaaccc actgtacctg cggataaagc tgcctgatta gcttgagcgc gtaactgatt
    39661 gcccttgatt cggagtttct cggcatacag tcatgcatcc atagccggtt aagctccata
    39721 tattctcggt tctgcgtacc cgttacgacg cgtgcaccac tgtttgggtt aagggcgtaa
    39781 ccccattgca ttacgccaac aagttcccgt tctgaaaata tacccagatg aaggtaggaa
    39841 ttatttacga aacggtggct gtaatgttta ttgacgataa ccagtctggc cagccagcaa
    39901 cttattgtct caacccgcaa ctcacgggag ccataaccaa cgatattgtc gttatagcga
    39961 ataagttcag gcgtgctgat gatacgggat gtgacttgct ttctgttccc cacgacagga
    40021 tttccttgtg tatgtggggt gctctgtggc gctcggagat gtgatttgat tgagggtttt
    40081 acagcgcgga cattttactt ccagataact gaaactggcc cgtgccagta gcttattgca
    40141 gtgtcggcac cgtatgtttc gatacatggc cgcataacct cctgctttgt tgttgatgcc
    40201 tttctcatgc aatcgatcga caaaaacgat cgattcgatt tgtttaattg atacaacaaa
    40261 tagatttaaa agtcatcaac aatgattcag ggcggtgatt atttacttaa agaaacgaag
    40321 agttaggtgg tgaaaacgat cataaaatga tcattttatt gactttttat acagtgaagg
    40381 taggatcgcg cgtatatgta gtatgtttca aatcaacagg atactacata aaagggaaac
    40441 ccagcatagc tggaactatg ctgggtcaat ctgtaaaaac ttgcatcaat gctttacttc
    40501 gagctcgtgg cgtatcctat gatattcccc ggtctcctgt aaagcagaga tgcataaaaa
    40561 ggagatcttt atcatggata aagagcctgt ttgccctgcc tgtaagactc ccttacagct
    40621 catctatcgc cgttctcgat ctcaaaatgg caagacatac aaaccaaaaa atgcttcctg
    40681 tttcaggatg ctgatatgtc cggcctgttc tccccggagg gaaaatacac aaaacgtgca
    40741 ttaacaaatt cacccggcag ggaaacctgc cggtcggtat tgttacatca tagtaccgtc
    40801 agttccgggt tgaaccggct ctggttgctc tacgggttga ggctcatttt ccgtgctgtt
    40861 ttcagccgga gaacgaccaa ccattcgggc cagcacttca tcaacatatg cgtcgatttg
    40921 tgcctcaaaa tccttgcgaa cctgcgcctt cagcacgcgg ttcacttctt cagaataaag
    40981 tgcctgtttg acaagctctt cagtaaccgt gcctttaatc tctggcatga atattctcct
    41041 ttccttttct ggttcacaaa aaattaaatc tgaccggagt ctttcggctg cggcattaca
    41101 gcaccgcttt ttatcttttc ctcggcagct gcatcaattt tatttcgaaa ataattgcga
    41161 ataattttat aaccaccgct gacaagcaac agacaacaaa ccaccgtgca gaaatacagc
    41221 aggcagacgt ttatatatgt catataagcc tcactcttat taactgatgt tacagccgga
    41281 taaattcccg gccgtcacga ttatgccgca ggggtattaa cctcaaggac aacgctctca
    41341 aaagacacgc cataagttgc ccctttggtg acatctgtca gtgtcagcgt atctttggca
    41401 ggggaaagtg catctttcac catctggaat gcctggcccg gagtgccatc cagcacaggc
    41461 gttacctggg cacttcccgc cgtaaacttc aggacaagtg tatgccactg gttattgtag
    41521 gctccaaatg atcccagttt cgtattcccc cctgttttat ggtgcatcag atttaactgg
    41581 ccgcctgttg tctggagata atacgccatc aggaacggtg tgcctgtacc actcatggtt
    41641 acaccttccg ggaccgtatc ggtgtggagg taaataccca gagcaaactg gccttctttt
    41701 acttcacctg acagacggaa tttacaggtc aactgaccac catgcttcag aagttcaccg
    41761 gctgcatcag ttccatgggt aagcgtccag gtttttccgc tttgtttgga tacggtcaac
    41821 gctttacctt cgtccgtcag ctggattgta ccatctgtaa ttttccagcc ctgagccggg
    41881 gcttctcctg ctgatgcttt aagtgaaagc agcgtacgga gtgacgggga tgtcggatgg
    41941 ggggactcac tgcctcctgg ctgactacct gtcggcgctt ccggtttccc acccggcgcg
    42001 ggctgaggca gggcttcacc atcagggggc acaccggcaa taaatgcaga ggtcagacct
    42061 gcatatttaa gaattgctga tgcaaaacga tctgagacca gaaaacggcg cgcccaggag
    42121 ctaaaatggg ttgccctgga tgctgttgtc cagttgccat ctccccgtga ttccgcacca
    42181 taatacccgg cagaagcgat atccgggtcg tgctccggca ggttggtcgc ggttttatta
    42241 ccgaactcat cgtgcatcag tggaacaaag tgaatgttct cggaagcccg gtttttatat
    42301 ccgctataaa tggtttcata tgccgccggg ttcttctctt tccagtcata ggttgtgtca
    42361 ccacataacc acggcacact gtctgatgaa ccaccaatac actgaccgga gaaagcacca
    42421 agatccgcac ggaatttttt caccatcgca gtaaactggg caccatgctg ggccggggtc
    42481 gctttaaggt ctccttcccc ctgaatccat acgacagata acagcacatt ctttggattc
    42541 gcctcaagtg ccgctttagt tcgactgaca agatcctgat acagtggggt atccactccc
    42601 cagcgtagtg acgcctcggc tgcaccactt gccggatcga atgagccaac tgcgccggtt
    42661 gtaaaagcag agccgccacg ggcacaggga accagtaaaa tcccggcatc agcaggaatc
    42721 accggtaata cttttttcgc aatatgaagt ccctgcccca cacaaccata ctgccctttt
    42781 ttcaggtctg cttttggatg gttcagcaga ctcatattct gaacatcatg aaggcagtga
    42841 tctgctggaa tgatttcatt gtatttgcag ggaatacctc cgggggtttg tgtattcctg
    42901 cgtgccagct gcataatacg tggatcagga cgatcataag aatccggaag gggcaaccct
    42961 tcaccataag atgaagcatt ggactggccg gctataacaa tcacgtaata ccaggaaggg
    43021 gagctggaag cagtttcatt tcctgccgga cgggtatgtg tttctgcatt atcccagtct
    43081 gacggttcag tagtaccggc aacatcagca cttaacggaa tgacagcacc aatacccgct
    43141 ccggtaattt ccacgttcgg atcaccggat actggcctta cccacaggca atcctgtgta
    43201 ttaaaactga tgagacagtc agcaaatgtc attcctcctt tcgtttcagc aggaggaaag
    43261 ggagcctcaa gaaatgccgc tgtaccaccg gaaactttaa cggtaaagtt tccgggcatt
    43321 gatgatagtt gaacccattt ggacgtcata atattcatcc tgttcctgaa taaacagaat
    43381 tatcatatcc acagaccgat aaacgccatt ttgcgttaat tcagtaaagg cccaacaaag
    43441 ggagcgtctt atttttccga aacgctcgca ggataataac gataaagggt tgaaaggccg
    43501 atatcaaaaa taagtgccac ctgtcttcga ctgacacccc cggccagtaa acgcccgacc
    43561 tgctcgtatt gttcctgcgt gagacgacgg ggtctgccgc cggttcgtcc cctttcacgg
    43621 gcagctgcaa gaccagcccg tgtccgttcg acaattaatt cacgctccat ttctgccagc
    43681 gcacccatta tgtgaaaaaa gaagcgcccc atgggggttg acgtatcaat gttgtcagta
    43741 agactacgaa aatttatccc ctgttgctgt aattcatctg tcagcgcaac aaggtggcgc
    43801 atactgcgcc ccagcctgtc cagtttccag accatcagcg tgtcaccggg ttttagtttc
    43861 ttcagtgccc gcttcagtcc cggccgctgc gttgtttttc cgctcatttt atcttcaaaa
    43921 atctgctcac atcctgcaca ttccagcgca tttctttgta aacctgtgtt ctggtcattt
    43981 gttgatacgc gtatatagcc aatcagcatg tatttacctt ccttaaaaag agatggaaag
    44041 gccttttaaa gcagatatac ggcatccctg aaacctcggt ttacgggata ctgtaaacag
    44101 ggccgcgaat gccctgccat caaacggtac ggctgttgca gcaagcaggc tggcaaatgc
    44161 ccgcagaatc aatggtgtcg cctttgacgg cacgcaggat ataaacatta cgtcaggaat
    44221 gccggaatca acggccaatc agcgttatgt tacaggggta agactgggag cgcaggcatt
    44281 aagcggcgga ctggaatata attactccct gtcatcagga aatgtgatta ccggttttaa
    44341 gactaacgga gactgggaaa tgcgtggtgg tgatgaccgt gtttattaca gacagataca
    44401 atattgtatt aatgggcatt gggtatccgc tgcaagtatt taattaacgg gggcatagca
    44461 cacagcagcc agcatctgaa atatgcgtta tttcagacaa gcattgcctg tatatggcga
    44521 tagtattatt cctgttttta tcattgattt acatggtgat gtttatgcaa ctgattaatg
    44581 tcaaacgata ttacccgaaa cacaaaccct atggtgagga tgttcagtat ttccagagtg
    44641 aggacggaag ggatttttat gaatctatcc cgcttttcac aaagaaatac aaactctgta
    44701 tttcgcctgt aacgggtatt atctgctctg ttgctgaaga tgtctctgct ttatatccgg
    44761 caggttttac tgttgttgaa gtggacgaac tgccggaagg tgtgaatatt gacggtaact
    44821 ggcagttttc tgatggcctg atatcaaagg tgccggtaaa ctggaagacg gttgctgaaa
    44881 agcgtcgctc atcgctgctc caggaagcca acgaaaccgt ggatgactgg aaaacagaat
    44941 taaaactgga catgattagc gatgaaaata aattacagct gacgcgctgg atggcgtata
    45001 tccgacagct taaagaaatg cactttaatg atattgccag tgaggggcat taccaggcga
    45061 ttccctggcc ggaaaaaccg gaataattca gagggtgcat tcagcaccct tttctgtggt
    45121 ttacactgaa cttaccgttc tccaggcatt attaatataa atctgaaccg ggcgataata
    45181 gaccccaccg atattgtccg cagaccggga gccggtttcc tgcacgttaa taccggaaag
    45241 catacaaccg gaaggtgcgc tgtaattcca tgatatttca ttgcctccgg gtgaatgata
    45301 gctctgtgct ccaagctgaa cattctgtac ataccggcca tctgcggttg actgcgtcat
    45361 tcctgacgtg atgttgatat cctgcgttcc gtcaaaagga acgccattaa tatttcttgg
    45421 ggtggcgagt ctgtttgctg ccacggcggt tccacccgat ggcagggcat cagcagcctt
    45481 atttaccgtt tcccgtaaac cgacgttatc gataaaagcg cctttatccg gaatgtctgc
    45541 gccgttctgc tccctgcgca ggtaacggtt atcaccttcg cttttgctgt aaacatccag
    45601 attattacgg gcggttcccg cattctccag gtcggcaagg ttctgatcct tcgccagagc
    45661 attaacgtca ccgggaccaa gactgttttt tgtggcaagc gccccaagac caagataacc
    45721 acgggcaaga cgctgggctt ccgcaccggc atcagcaatt tctttcaggt tgtttttccg
    45781 ggaaaaggca ttcgcagcga taatggcttt aatggacagc gtaagctggt ttaactgcgc
    45841 cttatccggc tgaatactgg cttcagccag aatattcagc agttcggcct ggagaatatt
    45901 cagccagtcc tgaccaatcc agctgatacc tttttgtccg tcaccttcgg taaaccaggt
    45961 ggtggtatta ctctgcgccg gtgccagcgc aggcatattc gccacgccgg aattattatc
    46021 aacatgaaac attaaatagt ctcctcttca tggctgtttg catacacgta aataaacgtc
    46081 tgccatgcag gcttataacg gttcagaata cattccagcg caccaccctc ataaatacgc
    46141 agcggggtga gaatatcatc cagcacattc atattccggt agccggtttc actgttaatc
    46201 gtgacaatac tgacccactg tgaatccggt gatggctgaa tatctatttc ataaccaaac
    46261 tctgccgcaa accggatata aaattcacgg ttaagagagg gtttcatccg gtatttattc
    46321 ccggcataac gctgacgctc ctgaatggtt gcgccggtca tatcacattc cggtaacccc
    46381 agataacgct cccagtcttc cagcagcaga cgggatgaat caggaaaacg ttctgccagc
    46441 atctggttac cggtccagga aacacgctcc gtggaatgac tgaggcccag gcacaaagcc
    46501 gcaagaacgg atgacggccg cttatcccat gccagcccgt caggcagcaa ctgcaacagg
    46561 gcacgctgat gcggggtcag ggttacagcc atgtgatttc ccccacggtc agcaactctg
    46621 tatctccggc catcacggac ttcagcgggc tgcgaacttc aaaatcctcc agtcctttca
    46681 cccctgcaac agcccgccag aatgatgaag gcaatacaag tccacccggc cgcgattcgt
    46741 tataaagcag gtccgttaat gcctgtttta cggcagcctg attttccggc gttttgggta
    46801 caatcctgat ctcaaacgcc accggcttat tggtcagctt aaacacgctg attgtcggcc
    46861 caagtggctg accaacgggc tggcccgtcg ccggatcatc atgactgcgg atataatccg
    46921 ccacccgctt cacatcacct tctccgggga aaatgtcagg gttattatcc tgaacaaaag
    46981 tcacaccaac actacccgcc tcaggccatt caggcttaca ccatgcccgc gtcacgcccg
    47041 gtacttcccg tgcccagcgt tcaaaatcat actgtgttcc ccctgatggc gggttctgta
    47101 cccggaatac cagcctggac agcaactccg gcaccgtttc cacatccgca ccaccggtga
    47161 gtcccgtgcc ggtgaccgtg gctgtctggt tgatgcccgc ctgtggcgtg ataaaggaca
    47221 ggcgggttcc ggtcggggta tttccggcgc gccccgcact ttccgcctcc acattaacat
    47281 tcagtgttcc ggcttttccg gtcgcagagc cggtgatgcg gtacacaaca ccatcgcttc
    47341 gctgaaggag tacgccttcc gtgatccctg catccgtggt cagcatcagt tgtaccggtc
    47401 cgtcagcgcg ggaagccggt ttacggatga caccccagaa tgcgcagtgt ttcaggagtt
    47461 cggcttcatc agcttctgtc ggaattatct gccgggagat ccaggccaga tgttcatgct
    47521 cctgtgctga cagacccgcg agtgcatacg caatggcatt cagggtggtt tcattcacgc
    47581 ccggctgcga accgggcagg cgctggctga tatcctgttg tgtctgtgtg atcagttgcg
    47641 ccagtggtaa gggctgataa ggcatcattc ccccttcata tcagcataaa aaatcatcgg
    47701 atgtctgctt ccgtccggca gggtaatttt cacggaaagc gaaaggcgag catgccccac
    47761 acgcatggca tgacatacca cggatgtggc agcaccgctt ttatgcagcc acgccagcgc
    47821 ttcatcagca taggcctgtg cacggctgac cacggaggac agcgtctttt cacggcttaa
    47881 cagccagaga cgggagccaa cggggcgctc ccggaaactg tctccccacc atccccgacg
    47941 gtcacgggtg ccgtcaggga tttcatcgga atccagcgcc cgcctgtccg taaacagtga
    48001 aataatgacc gcagtcagca ggctgttatc cgtcagaaga tctgcgccgt tcagttgcag
    48061 ggaaccgcaa ccctgatccc agacgacagc aatatcagcc attctctggt cctcccgttt
    48121 tgctgccttc accgttatcg tgatgcacat gacctgaata ggtgatcccg gcaataaccg
    48181 cttcggacat cgtgaacgtc ccctgtgatc ttccggtgcc gtcaatggac acattgcctt
    48241 tgacatgcag gttcttatcc actgtgacat taccggtaaa gtgagcctcc ggtgtatcca
    48301 cctgaacacc ttcatcggcg taaatctcca gcgtcttaca ggtcacaatg atccggccgt
    48361 tctttgtcag acgtatacgg tgcccctcat ggtgatacac gccggtatct ccggcggtaa
    48421 gtccggtcgg gcgactgcgg cggtcttcca ccacaagcac cacggcctga tcccgttgtc
    48481 cgccagcaca ggccagaaaa acttccgcac caggcagggg gacgctgatt tgcccgtact
    48541 gctgggggcg ttcaacgtca tcaaaggttt caccatccat accggttatt tgcacattct
    48601 ggattttcag cgtgtccgtt gtgccggtca gtacaccacg cccgaacagc agacgaacac
    48661 cccgcattac gggggccaga aggcggctga tgacttcgtc attcatcatt gaatttaatc
    48721 ccctgttttt tcatctgtgc ccgcacgaag gcgtccacgt cacccgcatt tccgcttccg
    48781 ctgtttcccg gctctgccgg aacaataaag ccgtcacggg gtgccagaat cagacgggtg
    48841 gtttcgccgt gatttgcatc cagcgaaaac tccacctgac agacaagaag atcacgctct
    48901 tccactccgg tgcggggagc cgtgacagcc gtcagcaggt tgatatccca caactggccg
    48961 ttctccctaa accagtgccg cacggtggct gtcaggcgca cagagcgggc aaccgcccgg
    49021 cgcatttccc ggacagccct ctggcgtgca ccgtctgcat caacagcatg atccgccagc
    49081 acaattttcg gtctgtaacg gtggatggca ctgtctgtca ccgtaccaac gggtgcggcc
    49141 agcgttccgg ctgtcagtgc atccccggca tgaccaccac cacgcccgtg cccccgtaca
    49201 cggtattcac tgtagcgcag acggtgatcc acgttgtgat ccaaatccag cagattttcg
    49261 cccagcgtca gcgtgtcgcc acgctgactg ccggcctgag tgaaaaccag ttcaccggcg
    49321 gcattactgg ttaccaggac gcctctgtgt ctcgcggccc ttgtcagcgc atctgccacg
    49381 gtttctgagt tttccagtgt gaaggtggaa aagggccggg ctgccgttgc atcatttacc
    49441 tgccagcgca ccgtgacccc gaacggagca cacaaatctg aagcaatgtg ctccagcgtg
    49501 cggttcctcc actggctgcc cggatgaatg gctgaacagt ccaccaggtc accggtttta
    49561 tcccgtccgc tgagcgtgat ctgaaaacgc gtggcgctga tgcgctgccg gacctgatcc
    49621 agccagccag tgatcacagg ctgaccgtca atgcgcaatt ccagagactg accggcacgg
    49681 acggatgacg gaaggcgtac accgggcatc attaccccca gttcaaagga gcccgccaga
    49741 tgttcaagag agcgacgaac actcaccgtc agccagccgg aaaaaatctc accgcccaga
    49801 tacagttcaa ccctgctgct cactaatcac ctccactgaa tggcctccgg gaatgaacag
    49861 cggatcaaca ataccgttac ggcgcacaaa acgttgccag ccggtactgt tcccggtggc
    49921 acggtacagg gtcaccagtg caggctcggt ggtacgtaca gtcactactg ttgcaccggg
    49981 tagctgaact ccccgtgtat tcagatcctc tgtcagtgcc agtctggcat cacgaagcgt
    50041 cagcgccgtg gcggtatacc cctgcccgga cagtgtcatc acctgccgtt cgagcgcttc
    50101 gcttaagtca cggttaatgc gctgaagatc aactgaactt tccagccaga caggccaggt
    50161 acggtgggct gcgtcactgt ccggcagggt ttcctgactg agaacctgat ccagcagttt
    50221 tcccacggtc tgtgcctggg ccaccacggc agcactctgc attaccgccc gtatcagacg
    50281 aatattcttt tgtgctgccg gtgtcagacc gatgagagtc tgcggattat cctgctcatc
    50341 cagcacgtaa cgcagggaag ataatgtcct gtacagttgc ggcattccct gagagacggc
    50401 ggatgtcgtc cggccgatac tgccaccggg ccgggtattc aggcctcctg aagacagtga
    50461 cggcagcgat ggcagggtga tcagggcact gaaaagcccc gcaaaagatg aggccatgcg
    50521 ggaaggtgct gtcagtaatg ccgtggcatt ccctttcagc gccgtgaacg tggcagtaaa
    50581 ggcactgata tcctgtacaa tccccatgcc ggaaacggca ttttccaggg cggacacctt
    50641 atcgctgatg gtttcggtca ttgcctggac atcgtgcatt ccgtcagaaa tcaccgtcca
    50701 gcccgcttcc agggtttcaa aaaccttccc gagcgcggtg gtactcttct gctccagaat
    50761 ggcggccgtg tcctgtgtga cagcaggtgc ggtatcatca cttaccggcg tgacattaat
    50821 ggtgaactca accacgccct gttcagcagc gttataacga ctttcaaagc tgtttatcag
    50881 cacatttaac gtgccgtaat ccgggtgaag cagctccccg gcaccaggag cacgaagggc
    50941 atcacgcaga cgatttcgct gtgtctgaac atcatccccc atcacaagta cactgaacgt
    51001 aaattcagcc agtttaggtc ccagatcgtc tgcaccaccg gtttcccgta acggatattc
    51061 ccgacggaca atgtttcgcc cgccacgttc gcgctgctca cgccagacga ggaacggcac
    51121 accacggaac gcaccacgac cgtcaccaga ggccataatt ccctccgttc cagacattca
    51181 catccagtcc gtgaccggac gtgtcatcaa tatcaatact gcgagcctgc cacccttccg
    51241 gggcaaccag ctccacacgg gcagaggctt tttgtaccgc ctgtccactg ctttcatcat
    51301 cactgccccg taatttctgc caggcttcag tcagccatcc ccccagatag ttaccgagat
    51361 agctgcccac cgtagaacca atggcaatcc ccacaggtcc ggctgccgcc cccagcgcac
    51421 caccggcaat actgcctgca agagagccga ccgcttctcc cttgtctgtg gcactggcgc
    51481 tgccatccag cagaaccggg gctgccatcg cgccagccca cagggcaccg ccacccagcc
    51541 ggccggccag tccaccggca ctgcggaaaa agccggagaa acgccccagc cccatgcggc
    51601 taccgacaga cgaaagtaac
//
LOCUS       DAZGXZ010000016        50527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000016 DAZGXZ010000000
VERSION     DAZGXZ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.016"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            125..2659
                     /gene="mrcB"
                     /locus_tag="ACHKJF_001083"
     CDS             125..2659
                     /gene="mrcB"
                     /locus_tag="ACHKJF_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP0948483.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2879..5068
                     /gene="fhuA"
                     /locus_tag="ACHKJF_001084"
     CDS             2879..5068
                     /gene="fhuA"
                     /locus_tag="ACHKJF_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP0948484.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            5119..5916
                     /gene="fhuC"
                     /locus_tag="ACHKJF_001085"
     CDS             5119..5916
                     /gene="fhuC"
                     /locus_tag="ACHKJF_001085"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP0948485.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5916..6806
                     /gene="fhuD"
                     /locus_tag="ACHKJF_001086"
     CDS             5916..6806
                     /gene="fhuD"
                     /locus_tag="ACHKJF_001086"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP0948486.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSESPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6803..8785
                     /gene="fhuB"
                     /locus_tag="ACHKJF_001087"
     CDS             6803..8785
                     /gene="fhuB"
                     /locus_tag="ACHKJF_001087"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP0948487.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFVLAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWT
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8820..10100)
                     /gene="hemL"
                     /locus_tag="ACHKJF_001088"
     CDS             complement(8820..10100)
                     /gene="hemL"
                     /locus_tag="ACHKJF_001088"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP0948488.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10266..10313
                     /gene="yadX"
                     /locus_tag="ACHKJF_001089"
     CDS             10266..10313
                     /gene="yadX"
                     /locus_tag="ACHKJF_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP0948489.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10325..11746
                     /gene="clcA"
                     /locus_tag="ACHKJF_001090"
     CDS             10325..11746
                     /gene="clcA"
                     /locus_tag="ACHKJF_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP0948490.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11770..11835
                     /gene="yadW"
                     /locus_tag="ACHKJF_001091"
     CDS             11770..11835
                     /gene="yadW"
                     /locus_tag="ACHKJF_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP0948491.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11828..12172
                     /gene="erpA"
                     /locus_tag="ACHKJF_001092"
     CDS             11828..12172
                     /gene="erpA"
                     /locus_tag="ACHKJF_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP0948492.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12219..12842)
                     /gene="yadS"
                     /locus_tag="ACHKJF_001093"
     CDS             complement(12219..12842)
                     /gene="yadS"
                     /locus_tag="ACHKJF_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP0948493.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12880..13680)
                     /gene="btuF"
                     /locus_tag="ACHKJF_001094"
     CDS             complement(12880..13680)
                     /gene="btuF"
                     /locus_tag="ACHKJF_001094"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP0948494.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13673..14371)
                     /gene="mtnN"
                     /locus_tag="ACHKJF_001095"
     CDS             complement(13673..14371)
                     /gene="mtnN"
                     /locus_tag="ACHKJF_001095"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP0948495.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14455..15972
                     /gene="dgt"
                     /locus_tag="ACHKJF_001096"
     CDS             14455..15972
                     /gene="dgt"
                     /locus_tag="ACHKJF_001096"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP0948496.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16102..17526
                     /gene="degP"
                     /locus_tag="ACHKJF_001097"
     CDS             16102..17526
                     /gene="degP"
                     /locus_tag="ACHKJF_001097"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP0948497.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            17681..18838
                     /gene="cdaR"
                     /locus_tag="ACHKJF_001098"
     CDS             17681..18838
                     /gene="cdaR"
                     /locus_tag="ACHKJF_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP0948498.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18927..19313)
                     /gene="yaeH"
                     /locus_tag="ACHKJF_001099"
     CDS             complement(18927..19313)
                     /gene="yaeH"
                     /locus_tag="ACHKJF_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP0948499.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19628..20452)
                     /gene="dapD"
                     /locus_tag="ACHKJF_001100"
     CDS             complement(19628..20452)
                     /gene="dapD"
                     /locus_tag="ACHKJF_001100"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP0948500.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20483..23155)
                     /gene="glnD"
                     /locus_tag="ACHKJF_001101"
     CDS             complement(20483..23155)
                     /gene="glnD"
                     /locus_tag="ACHKJF_001101"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP0948501.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23217..24011)
                     /gene="map"
                     /locus_tag="ACHKJF_001102"
     CDS             complement(23217..24011)
                     /gene="map"
                     /locus_tag="ACHKJF_001102"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP0948502.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24379..25104
                     /gene="rpsB"
                     /locus_tag="ACHKJF_001103"
     CDS             24379..25104
                     /gene="rpsB"
                     /locus_tag="ACHKJF_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP0948503.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25362..26213
                     /gene="tsf"
                     /locus_tag="ACHKJF_001104"
     CDS             25362..26213
                     /gene="tsf"
                     /locus_tag="ACHKJF_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP0948504.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26360..27085
                     /gene="pyrH"
                     /locus_tag="ACHKJF_001105"
     CDS             26360..27085
                     /gene="pyrH"
                     /locus_tag="ACHKJF_001105"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP0948505.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27377..27934
                     /gene="frr"
                     /locus_tag="ACHKJF_001106"
     CDS             27377..27934
                     /gene="frr"
                     /locus_tag="ACHKJF_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP0948506.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28026..29222
                     /gene="ispC"
                     /locus_tag="ACHKJF_001107"
     CDS             28026..29222
                     /gene="ispC"
                     /locus_tag="ACHKJF_001107"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP0948507.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29411..30169
                     /gene="ispU"
                     /locus_tag="ACHKJF_001108"
                     /gene_synonym="uppS"
     CDS             29411..30169
                     /gene="ispU"
                     /locus_tag="ACHKJF_001108"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP0948508.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30182..31039
                     /gene="cdsA"
                     /locus_tag="ACHKJF_001109"
     CDS             30182..31039
                     /gene="cdsA"
                     /locus_tag="ACHKJF_001109"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0948509.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31051..32403
                     /gene="rseP"
                     /locus_tag="ACHKJF_001110"
     CDS             31051..32403
                     /gene="rseP"
                     /locus_tag="ACHKJF_001110"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP0948510.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32433..34865
                     /gene="bamA"
                     /locus_tag="ACHKJF_001111"
     CDS             32433..34865
                     /gene="bamA"
                     /locus_tag="ACHKJF_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP0948511.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            34987..35472
                     /gene="skp"
                     /locus_tag="ACHKJF_001112"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             34987..35472
                     /gene="skp"
                     /locus_tag="ACHKJF_001112"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP0948512.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35476..36501
                     /gene="lpxD"
                     /locus_tag="ACHKJF_001113"
     CDS             35476..36501
                     /gene="lpxD"
                     /locus_tag="ACHKJF_001113"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP0948513.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36606..37061
                     /gene="fabZ"
                     /locus_tag="ACHKJF_001114"
     CDS             36606..37061
                     /gene="fabZ"
                     /locus_tag="ACHKJF_001114"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP0948514.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37065..37853
                     /gene="lpxA"
                     /locus_tag="ACHKJF_001115"
     CDS             37065..37853
                     /gene="lpxA"
                     /locus_tag="ACHKJF_001115"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP0948515.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37853..39001
                     /gene="lpxB"
                     /locus_tag="ACHKJF_001116"
     CDS             37853..39001
                     /gene="lpxB"
                     /locus_tag="ACHKJF_001116"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP0948516.1"
                     /translation="MTDQRSLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            38998..39594
                     /gene="rnhB"
                     /locus_tag="ACHKJF_001117"
     CDS             38998..39594
                     /gene="rnhB"
                     /locus_tag="ACHKJF_001117"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP0948517.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            39631..43113
                     /gene="dnaE"
                     /locus_tag="ACHKJF_001118"
     CDS             39631..43113
                     /gene="dnaE"
                     /locus_tag="ACHKJF_001118"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP0948518.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43126..44085
                     /gene="accA"
                     /locus_tag="ACHKJF_001119"
     CDS             43126..44085
                     /gene="accA"
                     /locus_tag="ACHKJF_001119"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP0948519.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44184..46325
                     /gene="ldcC"
                     /locus_tag="ACHKJF_001120"
     CDS             44184..46325
                     /gene="ldcC"
                     /locus_tag="ACHKJF_001120"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP0948520.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46382..46771
                     /gene="yaeR"
                     /locus_tag="ACHKJF_001121"
     CDS             46382..46771
                     /gene="yaeR"
                     /locus_tag="ACHKJF_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0948521.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46836..48134
                     /gene="tilS"
                     /locus_tag="ACHKJF_001122"
     CDS             46836..48134
                     /gene="tilS"
                     /locus_tag="ACHKJF_001122"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP0948522.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48183..48443)
                     /gene="rof"
                     /locus_tag="ACHKJF_001123"
     CDS             complement(48183..48443)
                     /gene="rof"
                     /locus_tag="ACHKJF_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP0948523.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48430..48630)
                     /gene="yaeP"
                     /locus_tag="ACHKJF_001124"
     CDS             complement(48430..48630)
                     /gene="yaeP"
                     /locus_tag="ACHKJF_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP0948524.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48796..49341
                     /gene="yaeQ"
                     /locus_tag="ACHKJF_001125"
     CDS             48796..49341
                     /gene="yaeQ"
                     /locus_tag="ACHKJF_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP0948525.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHMGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49338..49760
                     /gene="arfB"
                     /locus_tag="ACHKJF_001126"
     CDS             49338..49760
                     /gene="arfB"
                     /locus_tag="ACHKJF_001126"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP0948526.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLADSHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49774..50484
                     /gene="nlpE"
                     /locus_tag="ACHKJF_001127"
                     /gene_synonym="cutF"
     CDS             49774..50484
                     /gene="nlpE"
                     /locus_tag="ACHKJF_001127"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP0948527.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPLTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        11988 a        12363 c        14005 g        12171 t
ORIGIN      
        1 ctaaccctct ccctcaaggg agaggggacc gatcgagcac aaattttgag agatatcttc
       61 ttctgtcttg taacagaaga acagaaaatc gggcttttgc gcctgaatat tgcggagaaa
      121 aagcatggcc gggaatgacc gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt
      181 caaacaaaag gtaagccgtc gtcgttacga agatgacgat gattacgacg attatgatga
      241 ctatgaggat gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc
      301 tcgtggcaaa cgcggctggc tatggctgct gttaaaactg gctatcgttt ttgccgtgct
      361 gatcgccatt tacggcgttt atctcgatca aaaaattcgt agccgtattg atggcaaggt
      421 ctggcaactg cctgcggcag tttatggccg aatggtcaac cttgagccag atatgaccat
      481 cagcaagaac gagatggtga agctgctgga ggcgactcag tatcgtcagg tgtcgaaaat
      541 gacccgtcct ggcgaattta ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt
      601 tgatttcccg gacagtaaag aagggcaggt gcgtgcgcgt ctgacctttg atggcgatca
      661 tctggcgacg atcgtcaata tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc
      721 gcgtctgatc accatgatct cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag
      781 tggtttccct gatttgctgg tggatacttt gctggcgaca gaagaccgtc atttttacga
      841 gcatgacggc atcagcctct actccatcgg acgtgcggtg ctggcaaacc tgaccgccgg
      901 acgcacggtg cagggagcga gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc
      961 cagtgagcgt tcttactggc gtaaagcgaa cgaagcttac atggcgctga tcatggacgc
     1021 gcgttacagc aaagaccgta ttcttgagct gtatatgaac gaggtgtatc tcggtcagag
     1081 cggcgacaac gaaatccgcg gcttcccgct ggcgagcttg tattactttg gtcgcccggt
     1141 agaagagcta agcctcgacc agcaggcgct gttagtcggc atggtgaaag gagcgtccat
     1201 ctacaacccg tggcgtaacc caaaactggc gctggagcga cgtaatctgg tgctgcgtct
     1261 gctgcaacag caacagatta ttgatcaaga actctatgac atgttgagtg cacgtccgct
     1321 gggggttcag ccgcgcggtg gggtaatctc tccacagcca gcctttatgc aactggtgcg
     1381 tcaggagctg caggcaaaac tgggcgataa ggtaaaagat ctctccggcg tgaagatctt
     1441 cactaccttt gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc
     1501 ggcactgaag aaacagcgta agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg
     1561 ctttagtggt gaagttcgtg cgatggtcgg tggttctgag ccgcagtttg cgggctacaa
     1621 ccgtgcgatg caggcgcgtc gttcgattgg ttcccttgca aaaccagcga cttatctgac
     1681 ggctttaagc cagccgaaaa tctatcgtct gaatacgtgg attgcggatg cgccaatcgc
     1741 gctgcgtcag ccgaatggcc aggtctggtc accgcagaac gatgatcgtc gttatagcga
     1801 aagtggcaga gtgatgctgg tggatgcgtt gacccgttcg atgaacgtgc cgacggtaaa
     1861 tctggggatg gcgctggggc tgcctgcggt tacggagacc tggatcaaac tgggcgtgcc
     1921 gaaagatcag ttgcatccgg ttccggcaat gctgctgggg gcgttgaact taacgccaat
     1981 cgaagtggcg caggcattcc agaccatcgc cagcggtggt aaccgtgcac cgctttctgc
     2041 actgcgttcg gtaatcgcgg aagatggcaa ggtgctgtat cagagcttcc cgcaggcaga
     2101 acgcgctgtt ccggcgcagg cggcgtatct gacactatgg accatgcagc aggtggtaca
     2161 acgtggtacg ggtcgtcaac ttggggcgaa atacccgaac ctgcatctgg cagggaaaac
     2221 agggactacc aacaataacg tagatacttg gtttgcgggc attgacggca gcacggtgac
     2281 catcacctgg gtaggccgtg ataacaacca gccgaccaaa ctgtatggtg ccagcggggc
     2341 aatgtcgatt tatcagcgtt atctggctaa ccagacgcca acgccgctga atcttgttcc
     2401 gccagaagat attgcagata tgggcgtgga ctacgacggc aactttgttt gcagcggtgg
     2461 catgcgtgtc ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc agagcgagat
     2521 gcagcagcag ccgtcaggca atccgtttga tcagtcttct cagccgcagc aacagccgca
     2581 acagcaacct gctcagcaag agcagaaaga cagcgacggt gtagccggtt ggatcaagga
     2641 tatgtttggt agtaattaac atctaagcgt gaaataccgg atggcgaatt gccatccggt
     2701 aaaataacat cccacctaaa gtattaaccc tttcttttca tctggttgtt tattaaccct
     2761 tcaggaacgc tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa tattatctta
     2821 tctttataat aatcattctc gtttacgtta tcattcactt tacatcagag atataccaat
     2881 ggcgcgttcc aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc
     2941 cacagcggtt agcggcatgt ctgtttatgc acaggcagcg gttgaaccga aagaagacac
     3001 tatcaccgtt accgctgcac ctgcgccgca agaaagcgca tgggggcctg ctgcaactat
     3061 tgcggcgaag cactctgcta ctgcgactaa aacggataca ccaattgaaa aaacgccgca
     3121 gtctatttcg gttgtgacaa atgaagagat gcagatgcat caatttcagt ctgtaaaaga
     3181 agcattaggt tatacacctg gcgttacggt tagcagtcgt ggtgcttcta atacatatga
     3241 ttttgttatc attcgtggtt tctcatctgt tggtctgaat caaaataatt accttgatgg
     3301 actaaaactt cagggcaact tttataacga tgctgtgatt gatccttaca tgctcgagag
     3361 ggttgaactg atgcgtgggc cgacgtctgt tctctatgga aaaagtaatc ctggtgggat
     3421 tatttctatg gtgagtaagc gtccgaccac tgaacccctg aaagaaattc agtttaaaat
     3481 ggggacggat aatctgtttc agaccggatt cgatttcagt gatgcactgg atgataacgg
     3541 tgagttctct taccgtttga cgggccttgc tcgctcaaca aacgaacagc agaaaaactc
     3601 tgaatctcag cgttatacca ttgctccatc attctcatgg cgtccagacg acaaaaccaa
     3661 ttttactttc ctgtcctatt tccagaatga accagaaacg ggttattacg gttggttgcc
     3721 gaaagagggg accgttgagc cattgcctaa tggtaagcgt ctaccgactg acttcaatga
     3781 aggtgcatcg aataatacat actcccgtaa ccagaaaatg gtgggatata gttttgaaca
     3841 tggttttaat gacaccttca ccgtgcgtca gaatctgcgt ttcagtgaaa tgaaaacctc
     3901 acagaaaagt gtttatggca cagggattgc tggtgatggt cataccctaa accgcgggac
     3961 agtggtggat aatgagcgtc tacaaaactt tagcgttgat acccaacttg aaagtaagtt
     4021 tgctacaggt gacgtcgagc atactttgct gacaggggta gacttcatgc ggatgcgtaa
     4081 tgatatcaat gccagctttg gatccgcacc atccatcgat ctttataaca aatatcaccc
     4141 tgaatacttt gcatttggta gcgcagagcc ataccagatg aatgaaagca aacaaacagg
     4201 tatttatgtt caggatcagg cggaatggaa taaatgggta ttcactctgg ggggacgcta
     4261 cgattggtct aagcaagcga ccactgttcg tgaaaactct tatacgccga ctgaaggtta
     4321 tattgagcgc aatgatcatc agttcacctg gcgcggtggt gtaaattact tattcgataa
     4381 tggtatttca ccttacttta gctatagcca gtcctttgaa ccgagtgctt tcgatctgtg
     4441 gagcaacccg cgcatttcct ataagccatc gaaaggtgaa cagtatgaag ctggcgtaaa
     4501 atatgttccg aatgatatgc cggtcgttgt tacgggcgca gtctatcaat tgacgaaaac
     4561 aaataacctg acagcagacc caacaaaccc attagcgcaa gtcccagcag gtgagattcg
     4621 cgctcgtggt gtggaacttg aagcaaaagc ggcgttaaat gccaatatta acttgacggc
     4681 ttcttatacc tacaccgatg cggaatacac caaagacacc aatctcaaag gtaaaactcc
     4741 agaacaagta ccggagcata tggcatctct ctggggggat tataccttca atgaagggcc
     4801 gctttctggt ttaacattgg gaacaggtgg tcgttttatt ggttccagct atggtgatcc
     4861 ggcaaacact tttaaagtgg gtagcgcagc tgtaatggat gctgttgtaa aatatgatct
     4921 ggcacgcttt ggtatggcgg gatccagcct tgctgtgaac gtcaacaatt tgctcgatcg
     4981 tgagtatgtt gccagttgct tccagactta tggctgcttc tggggcgcag aacgtcaggt
     5041 cgttgcaacc gcaaccttcc gtttctaatt tctcttttgg ggcacggatt tccgtgccca
     5101 tttcacaagt tggctgttat gcaggaatac acgaatcatt ccgataccac ttttgcactg
     5161 cgtaatatct cctttcgtgt gcccgggcgc acgcttttgc atccgctgtc gttaaccttt
     5221 cctgccggga aagtgaccgg tctgattggt cacaacggtt ctggtaaatc cactctgctc
     5281 aaaatgcttg gccgtcatca gcctccgtca gaaggggaga ttcttcttga tgcccagccg
     5341 ctggaaagct ggagcagcaa agcgtttgcc cgcaaagtgg cttatttgcc gcagcagctt
     5401 cctccggcag aagggatgac cgtgcgtgaa ctggtggcga ttggtcgtta cccgtggcat
     5461 ggcgcgttgg ggcgctttgg ggcggcagat cgcgaaaaag tcgaggaagc tatctcgctg
     5521 gttggcttaa aaccgctggc gcatcggctg gtcgatagtc tctctggcgg cgaacgtcaa
     5581 cgggcgtgga tcgccatgct ggtggcgcag gatagctgtt gtctgttgct cgacgaaccg
     5641 acttcggcgc tggatatcgc ccaccaggtt gatgtgctgg cgctggtgca ccgtttaagt
     5701 caggagcgtg gcctgacggt cattgccgtg ttgcacgata tcaatatggc ggcacgctac
     5761 tgtgattatc tggtcgccct gcgcggtggt gaaatgattg ctcagggaac gcctgcggaa
     5821 attatgcgcg gcgaaactct cgaaatgatt tatggcatcc cgatgggtat tttgccgcat
     5881 ccggcgggtg ctgcacctgt gagttttgtt tattgatgag cggcttacct cttatttcgc
     5941 gccgtcgact gttaacggcg atggcgcttt ctccgttgtt atggcagatg aataccgccc
     6001 acgcggcggc tattgacccc aatcgtattg tggcgctgga gtggttgccg gtggaattac
     6061 tgctggcgct cggcatcgtg ccttacggcg tggcggatac catcaactat cgcctgtggg
     6121 tcagcgaatc accattgccg gactcagtga ttgacgtcgg tttgcgtaca gaacctaacc
     6181 ttgaactgct gaccgaaatg aaaccgtcgt ttatggtctg gtcggcagga tatggccctt
     6241 catcagaaat gctggcacgt attgcgccgg gtcgcggatt taacttcagc gatggcaaac
     6301 agccgttggc gatggcgcgt aaatcgctga cggaaatggc agatttactt aacctgcaaa
     6361 gcgcagcgga aacgcattta gcgcaatacg aagactttat ccgcagcatg aaaccccgct
     6421 ttgtgaagcg tggtgcacgc ccgttactgc tgacgacgct tatcgatccg cgccatatgc
     6481 tggtcttcgg tccaaacagc ttgttccagg aaattctcga tgagtacggc atcccaaatg
     6541 cctggcaagg ggaaaccaac ttctggggca gtaccgccgt cagtatcgat cgtctggcgg
     6601 cgtataaaga cgttgatgtg ctctgttttg atcacgacaa cagcaaagac atggatgcgc
     6661 taatggcaac cccgctgtgg caggccatgc cgtttgtccg cgccggacgc tttcagcgcg
     6721 tacccgcagt ctggttttat ggtgcgacgc tctcggcaat gcactttgtg cgcgttctgg
     6781 ataacgccat cggaggtaaa gcgtgagtaa acgaattgcg ctttttccgg cgttattgct
     6841 ggcgctgctg gtgattgtcg ctacggcgct gacctggatg aacttttcgc aggcgctgcc
     6901 gcgtagccag tgggcgcagg ccgcctggtc gccggatatt aacgtcatcg agcagatgat
     6961 ttttcactac agcttgttac cacgtctggc gatttcgctg ctggtgggcg caggcctggg
     7021 gctggtgggc gtgctgtttc agcaagtgct gcgtaatccg ctggcggaac cgacgacact
     7081 tggcgttgct acaggcgcgc aactgggaat taccgtcacc acactttggg cgatcccagg
     7141 tgctatggcg agccagtttg ccgcactggt gggcgcttgt gtcgttggct taatcgtctt
     7201 tggcgtcgcg tgggggaaac ggctttcgcc ggttacgctt attctcgcgg ggctggtggt
     7261 gagcctttat tgcggggcaa ttaatcagct actggttatc ttccatcacg accagctgca
     7321 aagcatgttc ctgtggagca ccggaacgct gacgcaaacc gactggggcg gcgttgagcg
     7381 tttatggccg cagttgctgg gcggtgtgat gctgacgttg ctgctacttc gtccgttaac
     7441 cctgatgggg cttgatgatg gcgtggcgcg caatctcggg ctggccttgt cgcttgcgcg
     7501 tctggcggcg ctgtcgctgg cgattgtcat cagtgcgctg ctggtgaacg ctgtggggat
     7561 tatcggcttt atcgggttgt tcgcgccgct gctggcaaaa atgctggggg cgcggcgtct
     7621 gctgccacga ctgatgctgg cgtcgttgat tggtgcgctg atcctctggc tttccgatca
     7681 aatcatcctc tggctgactc gcgtttggat ggaagtgtcc accggttcgg tcactgcgtt
     7741 gatcggtgcg ccgctgctac tgtggctgtt gccgcgttta cgcagcatta gcgcgccgga
     7801 tatgaaggtc aacgatcgtg tcgcggctga acgccaacat gtgctggcgt ttgtcctcgc
     7861 gggcggcgtg ctgctgttga tggctgtggt agtggcgctg tcgtttggtc gtgatgcgca
     7921 cggctggacg tgggcgagcg gggcgttgct cgatgattta atgccctggc gctggacgcg
     7981 aattatggcg gcgctgtttg cgggcgtcat gctggcggtg gcgggctgta ttattcagcg
     8041 gctgaccggc aacccgatgg caagcccgga agtgctgggg attagctccg gcgcagcgtt
     8101 tggcgtggtg ttgatgctgt ttctggtgcc gggtaatgcc tttggctggc tgttacctgc
     8161 aggcagtctc ggggcggcgg tgacgctgtt gatcattatg atcgccgccg gacgcggtgg
     8221 attttcccca caccgcatgt tactggcggg gatggcgtta agtaccgcgt tcaccatgct
     8281 tttgatgatg ttgcaggcaa gtggtgaccc gcgaatggcg caagtgctga cctggatttc
     8341 cggttcgacc tacaacgcga ccgatgcgca ggtctggcgc acgggaattg tgatggtaat
     8401 tttgctggcg attaccccgc tgtgccgccg ctggctgacc attttaccgc tgggtggtga
     8461 taccgcccga gccgtaggaa tggcgctgac gccgacgcga attgcgctgc tgctgttagc
     8521 ggcttgcctg acggcgaccg cgacgatgac tattggaccg ttgagttttg ttggtttaat
     8581 ggcaccgcat attgcgcgga tgatgggctt tcgacggacg atgccacaca tcgtaatttc
     8641 ggcgctggtg ggtggtttac tgctggtatt cgctgactgg tgtgggcgga tggtgctgtt
     8701 tccattccag atcccggcag ggctgctgtc gacctttatc ggcgcgccat attttatcta
     8761 tttgttgaga aagcagagcc gttaatagaa tgcctcgccc ggtgggcgag gcaaaatcat
     8821 cacaacttcg caaacacccg tcgtgcagca tcgatggtgt tattgatatc ttccatgctg
     8881 tgcgccacgg acataaagcc cgcttcaaac gctgacggtg ccaggtaaac accttcgtcc
     8941 agcatcatat ggaagaaacg cttaaagcgt tccacgtcac aggccatcac atcctgatag
     9001 cacgtcacgg actcggcgtc ggtaaagaaa ataccgaaca tgccgccaac gtggttaacg
     9061 accagcggaa ttccggcttc ttctgccgct tccagcagac cttctgccag acgtgttgtc
     9121 agctcatcca gcgtttcgtg aacgcccggc tgcgcgactt cattcagaca ggcgaaaccc
     9181 gctgccatcg caattgggtt accggaaagc gtacccgcct gatagaccgg acccgtcggg
     9241 gccagcgcat ccattacatc acgacgacca ccgaatgcgc ctaccggcat tccaccgccg
     9301 atgattttgc ccaggcaggt gagatccggt tccacaccgt aataatcctg tgcgccagct
     9361 agcgctacgc ggaagccagt cattacttca tcgatgatca gcaatgcgcc aaattcgtcg
     9421 cacagcgcgc gcagacctgg caggaactct ggcagcggtg gaacgcagtt catattgcct
     9481 gccaccggct caacgataat acaggcaatc tcttgcgggt attgctcaaa cgcggcgcgt
     9541 acagaagcca gatcgttata agtacaggtt aaggtatgtt tggcgaaatc tgccggaaca
     9601 cccggcgagt ttggctggcc taacgtgagt gcgccagaac cggctttcac cagcaggcag
     9661 tcagcgtgac cgtggtaaca accttcaaat ttaatgattt tgtcgcgacc ggtaaaacca
     9721 cgggccaggc ggatggcgct catcgtcgct tcagtgccgg agttcaccat gcgcaccata
     9781 tccatggtcg ggaccagttc ggtcaccagt tgcgccattt tcacttccat ttcggtcggt
     9841 gcaccaaagc ttaaaccacg ctcggcggct tcaatcacgg cattgcggat agccggatgg
     9901 ttatggccca gcaccatcgg cccccaggaa ccgacataat cgatataggc tttgccatca
     9961 acatcgtaca gataagcgcc gtccgctttt tcgataaaca gtggagtgcc gcccacgcca
    10021 gtaaaggcgc gaacagggga gttcacaccg ccagggatca gctcgcgcgc tgcgctgtaa
    10081 agattttcag acttactcat ggagggttcc tgattcgtag aaaaagtgaa tggctgctat
    10141 tctatgttat tcataacaag ttaaatactc gtcaaacatc aggctgcttg tactggtgca
    10201 atcctgattt cttagagtat aaaagttttg tgcatttgaa acattacgct ttgcaaagga
    10261 ttttcatgga acgtgcgagt aaaatgccgt catcttattt gtatgaccaa taagtgatca
    10321 ttggatgaaa actgatactc cctctttaga aacaccgcag gccgcgcgcc tgcgacgcag
    10381 acaactgatt cgccaacttc ttgagcgcga taaaaccccg ttagccattt tgtttatggc
    10441 ggcagtcgtc ggcacgcttg tcgggctggc tgcggttgct tttgacaaag gtgtcgcctg
    10501 gttgcagaac caacgtatgg gggcgctggt acatactgct gataattatc cgctactgtt
    10561 aaccgtcgct tttctctgtt cggcggtgct ggcgatgttc ggctactttt tggtgcgcaa
    10621 atacgcgccc gaagcagggg gatcggggat cccggaaatt gaaggggcgc tggaagatca
    10681 acgtcctgtt cgctggtggc gtgtattgcc ggtgaaattc tttggcgggc tggggacact
    10741 cggcggaggc atggtgctag ggcgcgaagg accaaccgtg cagatcggcg gcaatatcgg
    10801 tcgcatggtg cttgatattt tccgcctgaa aggtgatgaa gctcgccata cgctgctggc
    10861 aaccggtgct gctgcggggc tggctgcggc ctttaacgcg ccgctggcgg gtattttgtt
    10921 tattatcgaa gagatgcgtc cgcagtttcg ctatacgtta atttcgatta aagcggtatt
    10981 tattggcgtc attatgtcga ccattatgta ccggattttt aatcatgaag ttgcgttgat
    11041 tgacgtcggt aaactttctg atgcgccgct taatacgctg tggctttatc tgatcctcgg
    11101 tattattttt ggcattttcg gccctatttt taataaatgg gtgctgggga tgcaggattt
    11161 gctgcaccgt gtgcacggcg gcaatattac caaatgggtg ctaatgggcg gtgcgattgg
    11221 cggtctgtgt ggattgctgg ggtttgtggc accagcaacg tcgggcggcg gttttaacct
    11281 gattcctatc gctaccgcgg ggaatttcag catgggaatg ctggtgttta tcttcgtcgc
    11341 gcgggtcatt accaccttac tctgcttctc ttccggcgcg ccgggcggta tttttgcccc
    11401 gatgctggcg ctgggtactg tgctgggaac cgctttcgga atggttgccg ttgagctgtt
    11461 tccgcaatat caccttgagg cgggaacgtt tgctattgcc ggaatggggg cgttactggc
    11521 ggcatctatt cgcgcgccgt taacggggat cattctggtt ctggagatga ccgataacta
    11581 ccagctcatt ttgccaatga ttattaccgg tcttggcgca acactattag cgcaatttac
    11641 cggcgggaaa ccgctatact cggcgattct tgcgcgcacg ctggcaaaac aggaagctga
    11701 gcaactggcg cgaagcaagg ccgcatcagc cagcgagaat acttgaacga aataccaggg
    11761 tattagataa tggcgattat tattgggtta gaatttgccc aattgccgat gtcgtttgga
    11821 gcaaaatatg agtgatgacg tagcactgcc gctggagttt accgacgcag cagccaacaa
    11881 agttaaaagc ctgatcgctg acgaagataa cccgaatctg aaattacgcg tgtatatcac
    11941 cggtggcggt tgcagcggct tccagtatgg tttcaccttt gatgatcagg tgaacgaagg
    12001 cgatatgacc atcgaaaaac agggcgttgg cctggtggtt gatccgatga gcctgcaata
    12061 tctggtcggc ggttccgttg actataccga aggactggaa ggttctcgtt tcatcgtgac
    12121 caacccgaac gcgaaaagca cctgcggttg cggttcttcc tttagcatct aatctgttgt
    12181 tcatgtgccg gataaactat ccggcacatc ttctctcttc aacgcccatt ctcatccagc
    12241 gcaaacgtcg gtagcttaag atgccaacga atagccgcca gccgaatcaa tagcgtcacg
    12301 accatgccca tcatactggc tgtttccagt ggtacggaaa atgtgtaata agccgtagcg
    12361 tggacaatac cgccgataat acaggcagtt gcgtagattt ctgtacgtaa aatcatgggg
    12421 atctcgcggg ccagtacatc acgaataatc ccgccgccaa cgccagtaat gacgcccata
    12481 caaaccgcga ttaacggacc ggcttccgca ttaaaggctt tattcacgcc aatgccgaca
    12541 aacaccgcca gaccaacggc gtccagcacc ggcaacatcc attttggtaa gcgtcttggc
    12601 tggcgcacca gcacgatggt cagcatgctg gtgaccattg caacgaccag atcggtggga
    12661 tctttcaccc aaaataccgg gccgtgatcc agcgccatgt cgcgaattgt tccgccgcct
    12721 actgcggtta ccacgcccag taccagaaca ccaaaagggt ccatacgcaa ttttccggct
    12781 aacaaaacgc cggagatggc aaataccgct gtgccgacta tatccagcca atagacgagc
    12841 attgttcaat ccccactgag cacctgaaaa ggtcaggcac taatctacct gtgaaagcgc
    12901 attacagagc tgttgtgctg cgaggataat acgtgggctt gcacgttcaa accagtcact
    12961 cgtgagagga ataacgggaa ttttgagctg ttcaccccag tattgtttga ttttaggaat
    13021 ttggtccggt ccgcctgtaa tgacaatcgc ctgtggcgag cgtgctaaca cctgttcgcg
    13081 gctaacttgc ggccagggaa cccggctgtc tttaaagatg ttttctccgc cacaaacttc
    13141 gagtacctgg ttctgaatcg actcttttcc actggtaaat ggcggattaa tgccgaattg
    13201 cagaaaaaca cgttttttag gtttatcagc atattgcgct ttcaattgcg cgtactgatc
    13261 cagcagggat tgcgcggctt gttcggcctt gtctggttgc ggactccagg gggccagttg
    13321 acgtaacgca ttggcaattt gttcaatgct tgtcgcatcg acccacatca cttttattcc
    13381 cagcgaagcc agctggtcaa cctgccgctc ggcattacct ccacgccagg caatcaccag
    13441 atcgggcttc agcgcgacaa tgcgttccag attcatcccc tgccaggtgg aaacctgctc
    13501 aatcttttgc gcttgtggag gatagtcgga atagctgctg accccaaccg gcgtgatccc
    13561 ggcagcaaag gcaagttcag tgttggcggg agaaagcgtg atgacgcgcg gcgcggcgtt
    13621 gagccacagt ggcgcaagaa aagacagggc gaccagcgcc ctgaacagtg acttagccat
    13681 gtgcaagttt ctgcaccagt gactcaacca tcaggctgga ctgtttagcg gcaacagcca
    13741 ggaactcatc gaagctaaga tgagactgtt gatcggccac gtcggagatg gcgcgtacga
    13801 caacaaacgg gacgttgaaa ttgtggcaga catgggcgat tgccgtcgct tccatctcta
    13861 cagcaatggc ctgtgggaag ttgtggcgga ttttcgccag accaacagaa ccgttgatga
    13921 aagcgtcgcc gctaacaatc aggccacgta cagcgttaag attcagttcg gcaatgcagg
    13981 cctcagcggc agcgatcagt ttatcgtcag ctttaaagcc tgccggacag cctggtaact
    14041 gaccgtattc ataaccaaat gccgtgacat ccgcgtcgtg ataacgtgct tcgtccgaga
    14101 caacgatatc gcccactttc aacgttggtg ccaggccacc ggcagaaccg gtgttaataa
    14161 tcacatctgg cttgcagtgt tccaacagca aagtggcacc cagcgccgca gcgactttac
    14221 cgatgcccga tttcagaagc gcaacctcgg ttccattcag ttggccggta tagatttcgc
    14281 aaccgccgag actgatagtt tgacggtttt cgattttgtc acgcagcagc gtaacttctt
    14341 cttccattgc accaatgatg ccgattttca tagatttact cgcgataagc ccgatttgaa
    14401 ggcatagttt accatgcgct tacggggaag cgtatttctc acgcgggaga ggacatggca
    14461 cagattgatt tccgaaaaaa aataaactgg catcgtcgtt accgttcacc gcagggcgtt
    14521 aaaaccgaac atgagatcct gcggatcttc gagagcgatc gcgggcgtat catcaactct
    14581 ccggcaattc gtcgtctgca acaaaagacc caggtttttc cactggagcg caatgccgcc
    14641 gtgcgcacgc gtcttaccca ctcgatggaa gtccagcagg tggggcgcta catcgccaaa
    14701 gaaattttaa gccgtctgaa agagcttaaa ttgctggaag catacggcct ggatgaactg
    14761 accggacctt ttgaaagcat tgttgagatg tcatgcctga tgcacgatat cggcaatccg
    14821 ccgtttggtc attttggcga agcggcgata aatgactggt ttcgccagcg tttatacccg
    14881 gaagatgccg aaagccagcc tctgactgac gatcgctgca gcgtggcggc actacgttta
    14941 cgggacgggg aagaaccgct taacgagctg cggcgcaaga ttcgtcagga cttatgtcat
    15001 tttgagggga atgcacaagg cattcgtctg gtgcatacat tgatgaggat gaatctcacc
    15061 tgggcacagg ttggcggtat tttaaaatat acccgtccgg cgtggtggcg tggcgaaacg
    15121 cctgagacac atcactattt aatgaaaaag ccgggttatt atctttctga agaagcctat
    15181 attgcccggt tgcgtaaaga acttaatttg gcgctttaca gtcgttttcc attaacgtgg
    15241 attatggaag ctgccgacga catctcctat tgtgtggcag accttgaaga tgcggtagag
    15301 aaaagaatat ttaccgttga gcagctttat catcatttgc acgaggcgtg gggtcagcat
    15361 gagaaaggtt cgctcttttc gctagtggtt gaaaatgcct gggaaaaatc acgctcaaat
    15421 agtttaagcc gcagtacgga agatcagttt tttatgtatt tacgggtaaa caccctaaat
    15481 aaactggtac cctacgcggc acaacgattt attgataatc tgcctgcgat tttcgccgga
    15541 acgtttaatc atgcattatt ggaagatgcc agcgaatgca gcgatcttct taagctatat
    15601 aaaaatgtcg ctgtaaaaca tgtgtttagc catccagatg tcgagcagct tgaattgcag
    15661 ggctatcggg tcattagcgg attattagag atttatcgtc ctttattaag cctgtcgtta
    15721 tcagacttta ctgaactggt agaaaaagaa cgggtgaaac gtttccctat tgaatcgcgc
    15781 ttattccaca aactctcgac gcgccatcgg ctggcctatg tcgaggctgt cagtaaatta
    15841 ccgtcagatt ctcctgagtt tccgctatgg gaatattatt accgttgccg cctgctgcag
    15901 gattatatca gcggtatgac cgacctctat gcgtgggatg aataccgacg tctgatggcc
    15961 gtagaacaat aaccaggctt ttgtaaagac gaacaataaa tttttacctt ttgcagaaac
    16021 tttagttcgg aacttcgcgc tataaaacga atctgaagaa cacagcaatt ttgcgttatc
    16081 tgttaatcga gactgaaata catgaaaaaa accacattag cactgagtgc actggctctg
    16141 agtttaggtt tggcgttatc tccgctctct gcaacggcgg ctgagacttc ttcagcaacg
    16201 acagcccagc agatgccaag ccttgcaccg atgctcgaaa aggtgatgcc ttcagtggtc
    16261 agcattaacg tagaaggtag cacaaccgtt aatacgccgc gtatgccgcg taatttccag
    16321 cagttcttcg gtgatgattc tccgttctgc caggaaggtt caccgttcca gagctctccg
    16381 ttctgccagg ggggccaggg cggtaatggc ggcggccagc aacagaaatt catggcgctg
    16441 ggttccggcg tcatcattga tgccgataaa ggctatgtcg tcaccaacaa ccacgttgtt
    16501 gataacgcga cggtgattaa agtccagctg agcgatggtc gtaagttcga cgcgaagatg
    16561 gttggcaaag atccgcgctc tgatatcgcg ctgattcaga tccagaaccc gaaaaacctg
    16621 accgcaatta agatggcgga ttctgatgca ctgcgcgtgg gtgattacac cgtagcgatt
    16681 ggtaacccgt ttggcctggg agagacggta acttccggga ttgtctctgc gctggggcgt
    16741 agcggcctga atgccgaaaa ctacgaaaac ttcatccaga ccgatgcagc gatcaaccgt
    16801 ggtaactccg gtggtgcgct ggttaacctg aacggcgaac tgatcggtat caacaccgcg
    16861 atcctcgcac cggacggcgg caacatcggt atcggttttg ctatcccgag caacatggtg
    16921 aaaaacctga cctcgcagat ggtggaatac ggccaggtga aacgcggtga gctgggtatt
    16981 atggggactg agctgaattc cgaactggcg aaagcgatga aagttgacgc ccagcgcggt
    17041 gctttcgtaa gccaggttct gcctaattcc tccgctgcaa aagcgggcat taaagcgggt
    17101 gatgtgatca cctcactgaa cggtaagcca atcagcagct ttgccgcact gcgtgctcag
    17161 gtgggtacta tgccggtggg cagcaaactg accctgggct tgctgcgcga cggtaagcag
    17221 gtcaacgtga atctggaact gcagcagagc agccagaatc aggttgattc cagctccatc
    17281 ttcaacggca ttgaaggtgc tgagatgagc aacaaaggca aagatcaggg cgtggtagtg
    17341 aacaacgtga aaacgggcac tccggctgcg cagatcggcc tgaagaaagg tgatgtgatt
    17401 attggcgcga accagcaggc agtgaaaaac atcgctgaac tgcgtaaagt tctcgacagc
    17461 aaaccgtctg tgctggcact gaacattcag cgcggcgaca gctccatcta cctgttaatg
    17521 cagtaatctc cctcaacccc ttcctgaaaa cgggaagggg ttctccttac aatctgtgaa
    17581 cttcaccaca actccataca tcttcatcat cctttaggca tttgcacaat gccgtacgtt
    17641 acgtacttcc ttatgctaag ccgtgcataa cggaggactt atggctggct ggcatcttga
    17701 taccaaaatg gcgcaggata tcgtggcacg taccatgcgc atcatcgata ccaatatcaa
    17761 cgtaatggat gcccgtgggc gaattatcgg cagcggcgat cgtgagcgta ttggtgaatt
    17821 gcacgaaggt gcattgctgg tactttcaca gggacgagtc gtcgatatcg atgacgcggt
    17881 ggcacgtcat ctgcacggtg tgcggcaagg gattaatcta ccgttacggc tggaaggtga
    17941 aattgtcggc gtaattggcc tgacaggtga accagagaat ctgcgtaaat atggcgaact
    18001 ggtctgcatg acggctgaaa tgatgctgga acagtcgcgg ttgatgcact tgttggctca
    18061 ggatagccgt ttgcgggagg aactggtgat gaacctgatt caggcagagg agaatactcc
    18121 cgcacttact gaatgggcgc aacggctggg gatcgatctc aatcaaccgc gagtggtggc
    18181 tattgttgag gtcgacagcg gtcagcttgg tgtggacagc gcaatggcgg agttacaaca
    18241 actgcaaaac gcgctgacta cgcccgagcg taataatctg gtggcgattg tctcgctaac
    18301 cgaaatggtg gtgttgaaac cggcgttgaa ctcttttggg cgctgggatg cagaagatca
    18361 tcgtaagcga gttgaacaac tgattacccg catgaaagag tacggccagc tgcgttttcg
    18421 cgtttcactg ggcaactatt ttaccggtcc tggcagtatt gcccgatcct atcgtacggc
    18481 gaaaacgacg atggtggtgg gtaaacagcg gatgccagaa agtcgctgct atttttatca
    18541 ggatctgatg ttacctgtgt tactcgacag tttgcgtggc gactggcagg ccaacgaact
    18601 ggcgcgaccg ctggcgcggc tgaaagcgat ggacaataac ggcttgctgc gacgaacgct
    18661 ggcggcgtgg tttcgtcaca atgtgcaacc gctggcaacg tcaaaggcgt tgtttattca
    18721 tcgtaatact ctggagtatc ggcttaatcg tatatcggaa ctgaccgggc ttgatttggg
    18781 taattttgat gacaggttgc tgctgtatgt ggcgttgcag ctggatgaag agcggtaggt
    18841 tatgcgttaa ggtcgaggcg gcgctggctc attcccctca gcccgaaggg tagaggggaa
    18901 tgtccgagtt aaccgtcagt tcggcattat ttacgcgtta gtttttccag atcggcttca
    18961 atctcgctga tcttattggt tacgactgac tccaggtgac gtaagtcgtc gaggatctta
    19021 cgcttaagat caacttcgct gcggtcacgc tggcagattt gatcaagctc atcaatgata
    19081 taccgtagat tcgggctgat ttcctggact tctttataac cctgacccac accatcagcg
    19141 acgaccgttt tacgctgacg cggatattta aacttaacgc tcttggcgaa aaactcgcct
    19201 ttgtctttct ggaaatagat tttcagaata tcgttgttgg cttcctgccg gaggctgtaa
    19261 cgatcaattt cttcaggatt ggtaataccc agacttttca gattgtcgta catagcgtta
    19321 cctcaaaatg agtcagtaaa ttgtgcttat tttagcattt ggcctggccc gcccggctcg
    19381 actgtgatcg cagaaagcct ttttcttcca gacttctatc atggcgtaaa aatcaaaaaa
    19441 ttacctgctt tattgtggcg atattccatg ggatggatta acacatttat cttcgttttg
    19501 cgaattacat gacagtccga aacaagatga aaaagtattt aaaaaagggg ggaagaagaa
    19561 tgagtaggac gaattattct gagccagaaa gcatacgggc agcatcatgc tgcccgtata
    19621 tgtactctta atcgatggta cgcagcagtt cgttaatgcc gactttgccg cgagttttcg
    19681 cgtcaacttt cttaacgata accgcacagt agaggctgta tttgccatct ttcgacggca
    19741 gattacctga aacaaccaca gaccccgccg gaacgcgacc gtagtggatt tcgccggttt
    19801 cacggtcgta aatacgggtg ctctgaccaa tgtatacgcc catagagatg acggaacctt
    19861 cttcgacaat caccccttca accacttcgg agcgtgcgcc aatgaagcag ttatcttcaa
    19921 taatggtcgg gttagcctgc agcggttcca gcacaccgcc gatgcccacg ccaccggaaa
    19981 ggtggacgtt tttaccaatc tgcgcacaag aaccgacggt cgcccaggta tcaaccatgg
    20041 tgccttcatc aacatatgcg ccgatgttga cgtaagacgg catcagcacg gtgttacggg
    20101 caataaatgc accctgacgt accgccgctg gtggcacaac gcggaagcct tctttctgaa
    20161 aacgcgcttc atcgtagtcg gcgaatttca tcggcacttt atcgaagtag cggctttctg
    20221 ccccttcgat cacctgatta tcattaatac ggaaagagag cagcaccgct tttttcaacc
    20281 actgatgcgt cacccactga ccgtcaattt tttccgctac acgcagtgca ccggaatcca
    20341 gcagggcgat cacctgattt accgcttcgc gggtaacggt gtctgcgttg gctggcgtga
    20401 tctcggcacg gcgttcaaaa gcggtttcaa taatgttctg taactgctgc attgttaaac
    20461 tcttttcata tcagtaaaca catcaccctt tatcgtttgg attgagggcc tctgtcaacc
    20521 gctgatgcac ttcctgctgc aactcgttat taagtgcacg ccggtcagcg gtggcaatta
    20581 tgaataaatc ttctactcgc tcgccaatgg ttgtaattcg ggcaccatga agcgaaattc
    20641 ccagatcggc aaaaattttc ccgactctcg ccagcagtcc tggttggtcg agcgcgataa
    20701 gctcaaggaa cgatttgcgg tcggtatggg ttggcaaaaa cgtcacttcg gtttcaacag
    20761 taaagtgacg tagtttagcc ggttgacgac gcggctgtgg tggctgccag ctactttgtg
    20821 tcagtacctg ctccagacca aaccgaataa cctcatgacg atctgcggat agcgggctac
    20881 catcgggttc cagcacgatg aaggtatcca tcgccatacc gtcgcgagtg gtgaaaattt
    20941 gtgcgtcgtg aacacttaaa ttgcggcggt ctaattcggc acagacggcg gcaaacagat
    21001 aggggcggtc cgggctccag ataaaaatct cggtgcctcc acgcgtagcc tgcgggctaa
    21061 gcaataccag cggtttgctt aaatcatgct gtaataaatg gcgggcatgc caggccagtt
    21121 gatttgggct atggcggaca aaatagttag cacgacagcg tgaccaaatt tggtgcagcg
    21181 cctcttcgtc gatgttatcc atgcgcagta gtgccagtgc ctggagttgg tgatggcgaa
    21241 cccgttcgcg catatccggc gtgttttgca ttccgcgtcg tagctgtttt tcggtggcaa
    21301 aatagagttc gcgcaacaga ctttgcttcc agctattcca cagcgtttcg ttggtggcgc
    21361 aaatgtcagc cactgtcagg cataccagat aacgaagtcg gttttccgtt tgaacttctt
    21421 cggcaaactg cttgatgact tcagggtcct gaatatcgcg gcgttgtgct gtcaccgaca
    21481 tcagcaagtg ctggcgaacc agccaggcaa ccagctgtgt ttcgcgtgag ttcagcccgt
    21541 ggagttctgc aaaatgcact acatcctgag caccgagaat ggagtggtcg ccgccgcgtc
    21601 ctttggcgat atcgtgaaac agcgcggcga tgaaaatcag ctcagttgac ggcaggcgcg
    21661 gccagacgtc cacacacaac ggatggcgct ggcgcgtttc ttcactggca aaactctcca
    21721 gtttcagcat cacgcggata gtatgttcat ccaccgtgta ggcgtggaac agatcaaact
    21781 gcatctgccc gacgatatgc gaccattgcg gcatatacgc gccgagcacg ctatggcgat
    21841 gcattggcaa tagcccgcgc cgcaccgctc cggggtgacg cagaatgctc aaaaacagtt
    21901 ttcgtgcttc cggaatatta cacagcggtt gttgcagatg gcgacgggca tggcgtaact
    21961 ggcgcagcgt ggtggagtaa atgccggtga tcgcactgtt gcgcaccatg gtgtagaaca
    22021 tacgcaagat ggcttccggc tggcgcataa atagtgtttc atcacgcagg tcgattagcg
    22081 taccgcgtag ctgaaactca tcgtcgattg gacgtggttt ttcgtcggcg ggaagggcga
    22141 ggatggcttc atcgaacagt tgcagcagca tctggttgag ttcactgacg cggcgtgtaa
    22201 cgcggaagta atccttcatc atccgctcga ccggttcgtt gccttcacca ctgtaattca
    22261 gacgctgagc gacgctaagt tggcgatcga ataacagacg attatcgtaa cggctgacga
    22321 ccagatgcag ggcaaagcga atacgccaca gtatatgcag acattcgttt aattccgccc
    22381 gctccgctga ggttaagaag ccaaacccga ccatttcatc cagcgatgtt gcgccaaaat
    22441 gacggcgggc cacccattgc agagtgtgaa tatcgcgcaa gccgccaggg ctacttttga
    22501 tgtctggttc aaggttgtag ctggtgccat ggtaacgctg atggcgctgg ttctgttctt
    22561 caactttcgc cgcgtagaac ttgtcggaag gccagaatcc ttcgctaaaa atatgttttt
    22621 gcagttcgag gaagagcgca acatcgccaa ttaataagcg ggattcgatt aaattggtgg
    22681 cgacggttaa atccgataac ccttccagca tgcactcttc aagcgtgcgc acactatgac
    22741 cgacttccag ctttacatcc cagagcagcg ttaacagctc gcccactttt tgcgcctgat
    22801 cgtccgggag ctttttacgg cttaaaatca gtaaatcgat gtcagaaagt ggatgcagct
    22861 cgccacgacc atagccaccg acggcgacca atgccaggtc ggcaatctgg ctgaatcccg
    22921 cttcaatcca taatcgttgc aggagctggt cgataaactc ggtgcgcgcc tcaatcaact
    22981 gttctgcaga gatcccattg tcaaaggcat cacccagcca acgctggaaa gtatcgatat
    23041 gggcttttat cccaccgacg gttaattcat cacggggcca gacgcatgga ttttgcggtt
    23101 gaccgggcag ggtggggaga gcggtgtttg cgtactgttc tggaagggta ttcattgtgc
    23161 gccacccata agattaaatt atcgcattaa aaaagccggc attcgccggc ttcatcttat
    23221 tcgtcgtgcg agattatcgc cgggatggtg tcatccttgc gtagcgtcaa aatttcgcag
    23281 ccgttatcag tcaccacaat agtatgctca tattgtgcag acaagctgcg atctttggtt
    23341 tttaccgtcc agccatcttt catggtgcgg atctcttttt tacccgcgtt gaccattggc
    23401 tcaatggtga acgtcatccc cggtttcagc acgacgttgg tttcacggga gtcatagtgc
    23461 agcacctgcg gttcttcatg gaagccgcga ccaataccgt gtccacaata ttcacgaacg
    23521 acggagaagc cttctgcttc gacaaacttc tgaatcgccg caccgatttc gcgcaggtta
    23581 atgcctggtt tgaccatgcg cagcgccagg tacaggcttt cttgcgtgat gcggcacaga
    23641 cgttcgccca tgatggtcgg cttaccgacg ataaacattt tcgaggtatc gccgtggaaa
    23701 ccatctttga ttacggtgac atcaatgtta acgatatcgc catctttcag cagcttagca
    23761 tcgtccggga taccgtggca caccacttca ttaatagaga tgcaaacgga tttcggatag
    23821 ccgtgatagc cgaggcaggc agaaaccgcg tgttgttcat taacaatgta atcattacag
    23881 atgcgatcca gctcgccggt gctgacgccc ggtttaacat acggttcgat catctccagc
    23941 acttcggcag ccagtcggcc agcgacgcgc attttttcga tatcttctgg ggtcttgatt
    24001 gagatagcca ttaattctgt ccatcagcgt cggtgatacc gacaatatat atgtaagtgc
    24061 cgtcaatggt atcacacccg ggcaaattga gaatcattct gaatttcgcc aaacgtgcca
    24121 ctgaaggttt tctataatag aaaattcgac atctgatgct gtacacagcg ccaacaatta
    24181 ttggtgtcca tgacgtattt gtggtataaa gcgcgccgga cttccgatcc atttcgtata
    24241 cacagactgg acggaagcga caatctcact ttgtgtaaca acacacacgt atcggcacat
    24301 attccggggt gccctttggg gtcggtaata tgggatacgt ggaggcataa ccccaacttt
    24361 tatatagagg ttttaatcat ggcaactgtt tccatgcgcg acatgctcaa ggctggtgtt
    24421 cacttcggtc accagacccg ttactggaac ccgaaaatga agccgttcat cttcggtgcg
    24481 cgtaacaaag ttcacatcat caaccttgag aaaactgtac cgatgttcaa cgaagctctg
    24541 gctgaactga acaagattgc ttctcgcaaa ggtaaaatcc ttttcgttgg tactaaacgc
    24601 gctgcaagcg aagcggtgaa agacgctgct ctgagctgcg accagttctt cgtgaaccat
    24661 cgctggctgg gcggtatgct gactaactgg aaaaccgttc gtcagtccat caaacgtctg
    24721 aaagacctgg aaactcagtc tcaggacggt actttcgaaa agctgaccaa gaaagaagcg
    24781 ctgatgcgca ctcgtgagct ggagaaactg gaaaacagcc tgggcggtat caaagacatg
    24841 ggcggtctgc cggacgctct gtttgtaatc gatgctgacc acgaacacat tgctatcaaa
    24901 gaagcaaaca acctgggtat tccggtattt gctatcgttg ataccaactc tgatccggac
    24961 ggtgttgact tcgttatccc gggtaacgac gacgcaatcc gtgctgtgac cctgtacctg
    25021 ggcgctgttg ctgcaaccgt acgtgaaggc cgttctcagg atctggcttc ccaggcggaa
    25081 gaaagcttcg tagaagctga gtaataaggc ttgataactc ccccaaaata gttcgagttg
    25141 cagaaaggcg gcaagctcga gaattcccgg gagcttacat cagtaagtga ccgggatgag
    25201 cgagcgaaga taacgcatct gcggcgcgaa atatgaaggg ggagagccct tatagaccag
    25261 gtagtacacg tttggttagg gggcctgcat atggccccct ttttcacttt tatatctgtg
    25321 cggtttaatg ccgggcagat cacatctccg aggattttag aatggctgaa attaccgcat
    25381 ccctggtaaa agagctgcgt gagcgtactg gcgcaggcat gatggattgc aaaaaagcac
    25441 tgactgaagc taacggcgac atcgagctgg caatcgaaaa catgcgtaag tccggtgcta
    25501 ttaaagcagc gaaaaaagca ggcaacgttg ctgctgacgg cgtgatcaaa accaaaatcg
    25561 acggcaacta cggcatcatt ctggaagtta actgccagac tgacttcgtt gcaaaagacg
    25621 ctggtttcca ggcgttcgca gacaaagttc tggacgcagc tgttgctggc aaaatcactg
    25681 acgttgaagt tctgaaagca cagttcgaag aagaacgtgt tgcgctggta gcgaaaattg
    25741 gtgagaacat caacattcgc cgcgttgctg cgctggaagg cgacgttctg ggttcttatc
    25801 agcacggtgc gcgtatcggc gttctggttg ctgctaaagg cgctgacgaa gagctggtta
    25861 aacacatcgc tatgcacgtt gctgcaagca agccagaatt catcaaacca gaagacgtat
    25921 ccgctgaagt ggtagaaaaa gaataccagg tacagctgga tatcgcgatg cagtctggta
    25981 agccgaaaga aatcgcagag aaaatggttg aaggccgcat gaagaaattc accggcgaag
    26041 tttctctgac cggtcagccg ttcgttatgg aaccaagcaa aactgttggt cagctgctga
    26101 aagagcataa cgctgaagtg actggcttca tccgcttcga agtgggtgaa ggcatcgaga
    26161 aagttgagac tgactttgca gcagaagttg ctgcgatgtc caagcagtct taattatcaa
    26221 aaaggagccg cctgagggcg gcttcttttt gtgcccatct tgtaaattca gctaaccctt
    26281 gtggggctgc gctgaaaagc gacgtacaat gtcgctagta ttaattcatt tcaatcgttg
    26341 acagtctcag gaaagaaaca tggctaccaa tgcaaaaccc gtctataaac gcattctgct
    26401 taagttgagt ggcgaagctc tgcagggcac tgaaggcttc ggtattgatg caagcatact
    26461 ggatcgtatg gctcaggaaa tcaaagaact ggttgaactg ggtattcagg ttggtgtggt
    26521 gattggtggg ggtaacctgt tccgtggcgc tggtctggcg aaagcgggta tgaaccgcgt
    26581 tgtgggcgac cacatgggga tgctggcgac cgtaatgaac ggcctggcaa tgcgtgatgc
    26641 actgcaccgc gcctatgtga acgctcgtct gatgtccgct attccattga atggcgtgtg
    26701 cgacagctac agctgggcag aagctatcag cctgttgcgc aacaaccgtg tggtgatcct
    26761 ctccgccggt acaggtaacc cgttctttac caccgactca gcagcttgcc tgcgtggtat
    26821 cgaaattgaa gccgatgtgg tgctgaaagc aaccaaagtt gacggcgtgt ttaccgctga
    26881 tccggcgaaa gatccaaccg caaccatgta cgagcaactg acttacagcg aagtgctgga
    26941 aaaagagctg aaagtcatgg acctggcggc cttcacgctg gctcgtgacc ataaattacc
    27001 gattcgtgtt ttcaacatga acaaaccggg ggcgctgcgc cgtgtggtaa tgggtgaaaa
    27061 agaagggact ttaatcacgg aataattccc gtgatggata aataagggta agattccgcg
    27121 taagtatcgc gggggcgtta gtctggttat aaggcgttat tgttgcaggc agtttggtca
    27181 cggccagcgc gcagcaaccg gagcgtacaa aagtacgtga ggatggcgag cactgcccgg
    27241 ggccaaaatg gcaaataaaa tagcctaata atccagacga ttacccgtaa tatgtttaat
    27301 cagggctata cttagcacac ttccactgtg tgtgactgtc tggtctgact gagacaagtt
    27361 ttcaaggatt cgtaacgtga ttagcgatat cagaaaagat gctgaagtac gcatggacaa
    27421 atgcgtagaa gcgttcaaaa cccaaatcag caaaatacgc acgggtcgtg cttctcccag
    27481 cctgctggat ggcattgtcg tggaatatta cggcacgccg acgccgctgc gtcagctggc
    27541 aagcgtaacg gtagaagatt cccgtacact gaaaatcaac gtgtttgatc gttcaatgtc
    27601 tccggccgtt gaaaaagcga ttatggcgtc cgatcttggc ctgaacccga actctgcggg
    27661 tagcgacatc cgtgttccgc tgccgccgct gacggaagaa cgtcgtaaag atctgaccaa
    27721 aatcgttcgt ggtgaagcag aacaagcgcg tgttgcagta cgtaacgtgc gtcgtgacgc
    27781 gaacgacaaa gtgaaagcac tgttgaaaga taaagagatc agcgaagacg acgatcgccg
    27841 ttctcaggac gatgtacaga aactgactga tgctgcaatc aagaaaattg aagcggcgct
    27901 ggcagacaaa gaagcagaac tgatgcagtt ctgatttctt gaacgacaaa aacgccgctc
    27961 agtagatcct tgcggatcgg ctggcggcgt tttgcttttt attctgtctc aactctggat
    28021 gtttcatgaa gcaactcacc attctgggct cgaccggctc gattggttgc agcacgctgg
    28081 acgtggtgcg ccataatccc gaacacttcc gcgtagttgc gctggtggca ggcaaaaatg
    28141 tcactcgcat ggtagaacag tgcctggaat tctctccccg ctatgccgta atggacgatg
    28201 aagcgagtgc gaaacttctt aaaacgatgc tacagcaaca gggtagccgc accgaagtct
    28261 taagtgggca acaagccgct tgcgatatgg cagcgcttga ggatgttgat caggtgatgg
    28321 cagccattgt tggcgctgct gggctgttac ctacgcttgc tgcgatccgc gcgggtaaaa
    28381 ccattttgct ggccaataaa gaatcactgg ttacctgcgg acgtctgttt atggacgccg
    28441 taaagcagag caaagcgcaa ttgttaccgg tcgatagcga acataacgcc atttttcaga
    28501 gtttaccgca acctatccag cataatctgg gatacgctga ccttgagcaa aatggcgtgg
    28561 tgtccatttt acttaccggg tctggtggcc ctttccgtga gacgccattg cgcgatttgg
    28621 caacaatgac gccggatcaa gcctgccgtc atccgaactg gtcgatgggg cgtaaaattt
    28681 ctgtcgattc ggctaccatg atgaacaaag gtctggaata cattgaagcg cgttggctgt
    28741 ttaacgccag cgccagccag atggaagtgc tgattcaccc gcagtcagtg attcactcaa
    28801 tggtgcgcta tcaggacggc agtgttctgg cgcagctggg ggaaccggat atgcgtacgc
    28861 caattgccca caccatggca tggccgaatc gcgtgaactc tggcgtgaag ccgctcgatt
    28921 tttgcaaact aagtgcgttg acatttgccg caccggatta tgatcgttat ccatgcctga
    28981 aactggcgat ggaggcgttc gaacaaggtc aggcagcgac gacagcattg aatgccgcaa
    29041 acgaaatcac cgttgctgct tttcttgcgc aacaaatccg ctttacggat atcgctgcgt
    29101 tgaatttatc cgtactggaa aaaatggata tgcgcgaacc acaatgtgtg gacgatgtgt
    29161 tatctgttga tgcgaacgcg cgtgaagtcg ccagaaaaga ggtgatgcgt ctcgcaagct
    29221 gaggataatc cggctacaga gagtcgcgct atttgttagc gtagggcttc agtgatatag
    29281 tctgcgccat ctgatcgtaa gtagttggct ttataaggtc aggtatgccg tggttttaca
    29341 cggctttttt ttgtataggc ttcagtattc ctgagtaccg taaaccctgt cagggaataa
    29401 aaaacgcgtg atgttgtctg ctactcaacc acttagcgaa aaattgccag cgcatggctg
    29461 ccgtcatgtt gcgatcatta tggacggcaa tggtcgctgg gcaaaaaagc aagggaagat
    29521 tcgtgccttt gggcataaag ccggggcaaa atccgtccgc cgggctgtct cttttgcggc
    29581 caacaacggt attgaggcgt taacgctgta tgcctttagt agtgaaaact ggaaccgacc
    29641 agcgcaggaa gtcagtgcgt taatggaact gtttgtgtgg gcgctcgata gcgaagtaaa
    29701 aagtctgcac cgacataacg tgcgtctgcg tattattggc gataccagtc gctttaactc
    29761 gcgtttgcaa gaacgtattc gtaaatctga agcgctaaca gccgggaata ccggtctgac
    29821 gctgaatatt gcggcgaact acggtggacg ttgggatata gtccagggag tcaggcaact
    29881 ggctgaaaag gtgcagcaag gaaacctgca accagatcag atagatgaag agatgctaaa
    29941 ccagcatgtc tgtatgcatg aactggcccc tgtagattta gtaattagga ctggggggga
    30001 gcatcgcatt agtaactttt tgctttggca aattgcctat gccgaacttt actttacaga
    30061 tgttctctgg cccgatttcg atgaacaaga ctttgaaggg gcgttaaatg cctttgctaa
    30121 tcgagagcgt cgtttcggcg gcaccgagcc cggtgatgaa acagcctgat gggggtcgct
    30181 tttgctgaag tatcgcctga tatctgcttt tgtgttaata cccgtcgtca tcgcggcgtt
    30241 gtttctgttg ccgccggtgg ggttcgccat cgtaacgctg gtggtctgca tgctggcagc
    30301 gtgggaatgg ggacagctta gcggttttac cactcgttcg cagcgagtat ggttggcggt
    30361 gttatgcggg ttattgttgg cgctgatgct ttttctgttg ccggaatatc accgaaatat
    30421 tcatcaaccg ctggttgaaa tctcactttg ggcttcgctg ggttggtgga ttgtcgcgct
    30481 attgctggtg ctgttttacc caggttccgc agcaatctgg cgaaactcta aaacattgcg
    30541 ccttattttt ggcgtgctaa ccattgttcc cttcttctgg ggcatgctgg cgttacgggc
    30601 ctggcactat gacgagaatc attacagtgg cgcaatatgg ctgctctatg tcatgatcct
    30661 ggtatggggc gctgactctg gcgcatatat gtttggcaaa ttgtttggta aacataagct
    30721 ggcaccgaag gtttctccgg gtaaaacctg gcaaggcttt ataggtggac tagcgaccgc
    30781 agcggtaatc tcatggggtt atggcatgtg ggcgaatctc gacgtcgctc ccgtcacctt
    30841 actcatttgc tctattgtcg cagcgttagc ctcagtgctc ggcgatctga ccgagagtat
    30901 gtttaagcgt gaagcaggaa ttaaggacag cggtcattta attccaggac acggtggtat
    30961 tttagatcgt attgatagcc tgacggctgc ggtaccggtc tttgcttgct tgttgttact
    31021 ggtattcagg acgctttaac ggaaggtaat atgctgagtt ttctctggga tttggcttcg
    31081 ttcatcgttg cactgggtgt acttatcacc gtgcatgaat ttggtcattt ctgggttgcc
    31141 cggcgttgtg gtgttcgcgt tgagcgtttc tcaatagggt ttggtaaggc gctctggcgg
    31201 cgaactgata agctcggcac cgaatatgtt atcgccctga tcccgctggg cggttatgtc
    31261 aaaatgctgg atgagcgcgc agaaccggtc gttccggaac tccgccacca tgccttcaat
    31321 aataaatctg ttggccaacg agcggcgatt attgccgcag gtccggttgc aaacttcatt
    31381 tttgctatct ttgcctactg gctggttttt attattggtg tgcctggcgt acgtccggtg
    31441 gttggtgaaa tagcagccaa ttcgatagct gcggaagcac aaattgcacc aggtacggaa
    31501 ctaaaagccg tagatggtat cgaaacgcct gattgggatg ccgtgcgttt gcagttggtc
    31561 gataaaattg gcgatgaaag caccaccatt acagtagcgc catttggcag cgatcaacgg
    31621 cgggatgtaa agctcgattt acgtcactgg gcgtttgagc ctgataaaga agatccggta
    31681 tcttcgctgg ggattcgtcc tcgtgggccg caaattgaac ctgtactgga aaatgtgcag
    31741 ccaaactcgg cggcaagcaa ggcaggtttg caagcaggcg acaggatcgt taaagtcgat
    31801 ggtcagccct taacgcagtg ggtgaccttt gtgatgcttg tccgggataa cccgggtaaa
    31861 tccttagcgt tagaaatcga aaggcaggga agtcccttgt ctttgacatt aatcccggag
    31921 agtaaaccgg gtaatggtaa agcgattggt tttgtcggta ttgagccgaa agtcattcct
    31981 ttgccagatg agtataaagt tgtacgccag tatgggccgt tcaacgccat cgtcgaagcc
    32041 acggacaaaa cgtggcagct gatgaagctg acggtcagta tgctgggaaa attgatcacc
    32101 ggtgatgtga aactgaacaa cctcagtggg ccgatctcta tcgccaaggg ggctgggatg
    32161 acagcggaac tcggggtagt ttattacctg ccgtttcttg cgcttattag cgtgaactta
    32221 gggataatta acctgtttcc gttgcccgta cttgacgggg ggcatctgct gttccttgcg
    32281 atcgaaaaga tcaagggcgg accggtatcc gagcgggttc aagacttttg ttatcgcatt
    32341 ggctcgattc tgctggtgct gttaatgggg cttgcacttt tcaatgattt ctctcggtta
    32401 tgagagttag ttaggaagaa cgcataataa cgatggcgat gaaaaagttg ctcatagcgt
    32461 cgctgctgtt tagcagcgcc accgtatacg gtgctgaagg gttcgtagtg aaagatattc
    32521 atttcgaagg ccttcagcgt gtcgccgttg gtgcggccct cctcagtatg ccggtgcgca
    32581 caggcgacac ggttaatgat gaagatatca gtaataccat tcgcgctctg tttgctaccg
    32641 gcaactttga ggatgttcgc gtccttcgtg atggtgatac ccttctggtt caggtaaaag
    32701 aacgtccgac cattgccagc attactttct ccggtaacaa atcggtgaaa gatgacatgc
    32761 tgaagcaaaa cctcgaggct tctggtgtgc gtgtgggcga atccctcgat cgcaccacca
    32821 ttgccgatat cgagaaaggt ctggaagact tctactacag cgtcggtaaa tatagcgcca
    32881 gcgttaaagc tgtcgtgacc ccgctgccgc gcaaccgtgt tgacctaaaa ctggtgttcc
    32941 aggaaggtgt gtcagctgaa atccagcaaa ttaacattgt tggtaaccat gctttcacca
    33001 ccgacgaact gatctctcat ttccaactgc gtgacgaagt gccgtggtgg aacgtggtag
    33061 gcgatcgtaa ataccagaaa cagaaactgg cgggcgacct tgaaaccctg cgcagctact
    33121 atctggatcg cggttatgcc cgtttcaaca tcgactctac ccaggtcagt ctgacgccag
    33181 ataaaaaagg tatttacgtc acggtgaaca ttaccgaagg cgatcagtac aagctttctg
    33241 gcgttgaagt gagcggcaac cttgccgggc actccgctga aattgagcag ctgactaaga
    33301 tcgagccggg cgagctgtat aacggcacca aagtgaccaa gatggaagat gacatcaaaa
    33361 agcttctcgg tcgctatggt tatgcctatc cgcgcgtaca gtcgatgcct gaaattaacg
    33421 atgccgacaa aaccgttaaa ttacgcgtga acgttgatgc gggtaaccgt ttctacgtgc
    33481 gtaagatccg ttttgaaggt aacgatacct cgaaagatgc cgtcctgcgt cgcgaaatgc
    33541 gtcagatgga aggtgcatgg ctgggaagcg atctggtcga tcagggtaag gagcgtctga
    33601 atcgtctggg cttctttgaa actgtcgata ccgataccca acgtgttccg ggtagcccgg
    33661 accaggtaga tgtcgtctac aaggtaaaag agcgcaacac cggtagcttc aactttggta
    33721 ttggttacgg tactgaaagt ggcgtgagct tccaggctgg tgtgcagcag gataactggt
    33781 taggtacagg ttatgctgtt ggtatcaacg ggaccaaaaa cgattaccag acctatgctg
    33841 aactgtcggt aaccaacccg tacttcactg tagatggtgt aagcctcggt ggtcgtctct
    33901 tctataatga cttccaggca gatgacgccg acctgtccga ctataccaac aagagttatg
    33961 gtacagacgt gacgttgggc ttcccgatca acgaatataa ctcgctgcgt gcaggtctgg
    34021 gttatgtaca taactccctg tccaacatgc agcctcaggt tgcgatgtgg cgttatctgt
    34081 actctatggg tgaacatccg agcacctctg atcaggataa tagcttcaaa acggacgact
    34141 tcacgttcaa ctatggttgg acctataaca agcttgaccg tggttacttc ccgacagatg
    34201 gttcacgtgt caacctgacc ggtaaagtga ccattcctgg atcggataac gaatactaca
    34261 aagtgacgtt agacacggcg acttatgtgc cgatcgatga cgatcacaaa tgggttgttc
    34321 tggggcgtac ccgctggggt tatggtgatg gtttaggcgg caaagagatg ccgttctacg
    34381 agaacttcta tgccggtggt tccagcaccg tgcgtggctt ccagtccaat acaattggtc
    34441 cgaaagcagt ttacttcccg catcaggcca gtaattatga tccggactat gattacgaat
    34501 gtgcgactca ggacggcgcg aaagacctgt gtaaatcgga tgatgctgta ggcggtaacg
    34561 ccatggcggt tgccagcctc gagttcatca ccccgacgcc gtttattagc gataagtatg
    34621 ctaactcggt tcgtacttcc ttcttctggg atatgggtac cgtttgggat acaaactggg
    34681 attccagcca atattctgga tatccggact atagtgatcc aagcaatatc cgtatgtctg
    34741 cgggtatcgc attacaatgg atgtccccat tggggccgtt ggtgttctcc tacgcccagc
    34801 cgttcaaaaa gtacgatgga gacaaggcag aacagttcca gtttaacatc ggtaaaacct
    34861 ggtaagtgtt ctccacaaag gaatgtagtg gtagtgtagc gatgacttta ggcgatcaat
    34921 ataagatcgc cgggccacgc aaagaactgc accctccggt gcaaatggga tggtaaggag
    34981 tttattgtga aaaagtggtt attagctgca ggtctcggtt tagcactggc aacttctgct
    35041 caggcggctg acaaaattgc aatcgtcaac atgggcagcc tgttccagca ggtagcgcag
    35101 aaaaccggtg tttctaacac gctggaaaat gagttcaaag gccgtgccag cgaactgcag
    35161 cgtatggaaa ccgatctgca ggctaaaatg aaaaagctgc agtccatgaa agcgggcagc
    35221 gatcgcacta agctggaaaa agacgtgatg gctcagcgcc agacttttgc tcagaaagcg
    35281 caggcttttg agcaggatcg cgcacgtcgt tccaacgaag aacgcggcaa actggttact
    35341 cgtatccaga ctgctgtgaa atccgttgcc aacagccagg atatcgatct ggttgttgat
    35401 gcaaacgccg ttgcttacaa cagcagcgat gtaaaagaca tcactgccga cgtactgaaa
    35461 caggttaaat aagtaatgcc ttcaattcga ctggctgatt tagcgcagca gttggatgca
    35521 gaactacacg gtgatggcga tatcgtcatc accggcgttg cgtccatgca atctgcacaa
    35581 acaggtcaca ttacgttcat ggttaaccca aaataccgtg agcatttagg cttgtgccag
    35641 gcgtccgcgg ttgtcatgac ccaggacgat cttcctttcg cgaaaagtgc cgcgctggta
    35701 gtgaagaatc cctacctgac ttacgcgcgc atggcgcaaa ttttagatac cacgccgcag
    35761 cccgcgcaga acattgcacc cagtgcggtg atcgacgcga cggcgaagct gggtaacaac
    35821 gtatcgattg gcgctaacgc ggtgattgag tccggcgttg aactgggcga taacgtgatt
    35881 atcggtgccg gttgcttcgt aggtaaaaac agcaaaatcg gtgcaggttc gcgtctctgg
    35941 gcgaacgtaa ccatttacca tgagatccag atcggtcaga attgcctgat ccagtccgga
    36001 acagtggtag gcgcagacgg ctttggttat gccaacgatc gtggtaactg ggtgaagatc
    36061 ccacagattg gtcgcgtaat tattggcgat cgcgtggaga tcggtgcctg cacaaccatc
    36121 gatcgcggcg cgctggatga cactattatt ggcaatggcg tgatcattga taaccagtgc
    36181 cagattgcac ataacgtcgt gattggcgac aatacggcgg ttgccggtgg cgtcattatg
    36241 gcgggcagcc tgaaaattgg tcgttactgc atgatcggcg gagccagcgt aatcaacggg
    36301 catatggaaa tatgcgacaa agtgacggtt acgggcatgg gtatggtgat gcgtcccatc
    36361 actgaaccag gcgtctattc ctcaggcatt ccgctgcaac ccaacaaagt ctggcgcaaa
    36421 accgctgcac tggtgatgaa cattgatgac atgagcaagc gtctgaaatc gcttgagcgc
    36481 aaggttaatc aacaagacta acgttccatc ttttgttcgc caaactttac ggcctgtctc
    36541 attcttacga ttgcggcagg ccgtgttatt attgtcgttt cttatatttt gacaggaaga
    36601 gtatcttgac tactaacact catactctgc agattgaaga gattttagaa cttctgccgc
    36661 accgtttccc gttcttactg gtggatcgcg tgctggattt tgaagaaggt cgttttctgc
    36721 gcgcagtaaa aaatgtctct gtcaatgagc cattcttcca gggccatttc cctggaaaac
    36781 cgattttccc gggtgtgctg attctggaag caatggcaca ggcaacaggt attctggcgt
    36841 ttaaaagcgt aggaaaactg gaaccgggtg aactgtacta cttcgctggt attgacgaag
    36901 cgcgcttcaa gcgcccggtc gtgcctggcg atcaaatgat catggaagtc actttcgaaa
    36961 aaacgcgccg cggcctgacc cgttttaaag gggttgctct ggtcgatggt aaagtagttt
    37021 gcgaagcaac gatgatgtgt gctcgtagcc gggaggcctg atacgtgatt gataaatccg
    37081 cctttgtgca tccaaccgcc attgtggaag agggcgcgtc aattggcgcg aacgcacaca
    37141 ttggtccttt ttgtatcgtt ggaccccatg tcgaaattgg tgagggtacc gtactgaaat
    37201 ctcacgttgt cgtgaatggt catactaaaa ttggccgcga taatgagatt tatcagttcg
    37261 cctccatcgg cgaagttaac caggatttga aatatgctgg cgaaccgacc cgtgtggaaa
    37321 tcggcgatcg taaccgcatt cgcgaaagcg tcaccattca tcgtggcaca gtccagggcg
    37381 gtggattgac gaaggtgggc agcgacaact tactgatgat caacgcgcac attgcgcacg
    37441 attgtacggt aggtaaccgc tgtattctcg ccaacaacgc aacgctggcg ggtcacgtat
    37501 cggttgacga cttcgcgatc atcggcggca tgaccgcagt ccatcagttc tgcatcattg
    37561 gtgcgcatgt gatggttggc ggctgctccg gtgtggcgca ggacgtccct ccttatgtta
    37621 ttgcgcaggg taaccacgca acgccgttcg gtgtcaatat cgaagggctg aagcgccgcg
    37681 gattcagccg tgaggcgatt accgctatcc gcaatgcgta taagctgatt tatcgtagcg
    37741 gtaaaacgct cgatgaagtg aaaccggaaa ttgctgaact ggcggaaaca tatccggaag
    37801 tgaaagcctt taccgatttc tttgcacgct caacgcgcgg tctgattcgt taatgactga
    37861 ccagcgttca ttaacgattg ccctggtcgc cggagaaacc tccggcgata tcctgggggc
    37921 cggtttaatc cgcgctctga aagaacgtgt gcccaacgcc cgctttgttg gtgttgccgg
    37981 gccacgaatg caggctgaag gctgcgaagc ctggtacgaa atggaagaac tggcggtgat
    38041 gggcattgtt gaagtgctcg gtcgtctgcg tcgcttactg catattcgtg ccgatctgac
    38101 aaagcgtttt ggcgaactga agccagatgt ttttgttggt attgatgcgc ccgacttcaa
    38161 tattactctt gaaggtaacc tcaaaaagca gggtatcaaa accattcatt acgtcagtcc
    38221 atccgtctgg gcgtggcgac agaaacgcgt tttcaaaata ggcagagcca ccgatctggt
    38281 gctcgcattt ctgcctttcg aaaaagcgtt ttatgacaaa tacaatgtac cgtgccgctt
    38341 tatcggtcat accatggctg atgccatgcc tttagatcca gataaaaatg ccgcccgtga
    38401 tgtgctgggg atccctcacg atgcccactg cctggcgttg ctaccgggga gccgtggtgc
    38461 agaagtcgaa atgcttagcg ccgatttcct gaaaactgcc cagcttttgc gccagacata
    38521 tccggatctc gaaatcgtgg tgccgctggt gaatgccaaa cgccgcgagc agtttgaacg
    38581 catcaaagct gaagtcgcgc cagacctgtc ggttcatttg ctggatggaa tgggccgtga
    38641 ggcgatggtc gccagcgatg cggcactact ggcgtcgggg acggcagccc tggagtgtat
    38701 gctggcgaaa tgcccgatgg tggtgggata tcgcatgaag cctttcacct tctggctggc
    38761 gaagcgactg gtgaaaactg attatgtctc gctgccaaat ctgctggcgg gcagagagtt
    38821 agtcaaagag ttattgcagg aagagtgtga gccgcaaaaa ctggctgcgg cgctgttacc
    38881 gctgttggcg aacgggaaaa ccagccacgc gatgcacgat accttccgtg aactgcatca
    38941 gcagatccgc tgcaatgccg atgagcaggc ggcacaagcc gttctggagt tagcacaatg
    39001 atcgaatttg tttatccgca cacgcagctg gttgcgggtg tggatgaagt cggacgcggg
    39061 ccgttagttg gcgcggtcgt caccgctgcg gtgatccttg acccggcgcg cccgattgcc
    39121 gggctgaatg attccaaaaa gctgagcgaa aaacgtcgtc tggcgctctg tgaagagatc
    39181 aaagagaaag cgttgagctg gagtctgggc cgcgcggaac ctcacgaaat cgacgagctg
    39241 aacattcttc atgcgaccat gctggcgatg cagcgtgccg tcgctgggct gcatattgcg
    39301 ccggaatatg tgttgattga tggtaaccgc tgcccgaaat taccgatgcc tgcgatggct
    39361 gtggtgaaag gcgatagccg cgtaccggaa atcagtgccg cgtctatcct ggcgaaagtg
    39421 acgcgtgacg ccgaaatggc ggcgctggat attgttttcc cgcaatatgg ttttgcccaa
    39481 cacaaagggt acccaaccgc ttttcatctg gaaaaactgg ctgaacacgg cgcgaccgaa
    39541 caccatcggc gcagctttgg gcctgtcaaa cgcgcactgg gacttgcgtc ctgattcttg
    39601 tgtcgagatt aagtaaaccg gaatctgaag atgtctgaac cacgtttcgt acacctgcgg
    39661 gtgcacagcg actactcgat gatcgatggc ctggccaaaa ccgcaccgtt ggtaaaaaag
    39721 gcggcggcgt tgggtatgcc agcactggcg atcaccgatt tcaccaacct ttgtggtctg
    39781 gtgaagttct acggagcggg acatggcgca gggattaagc ctatcgtcgg ggcagatttt
    39841 aacgtccagt gcgacctgct gggtgatgag ttaacccacc tgacggtact ggcggcgaac
    39901 aataccggct atcagaatct gacgttgctg atctcaaaag cgtatcagcg cgggtacggt
    39961 gccgccgggc cgatcatcga tcgcgactgg cttatcgaat taaacgaagg gttgatcctt
    40021 ctttccggcg gacgcatggg cgacgtcgga cgcagtcttt tgcgtggtaa cagcgcgctg
    40081 gtagatgagt gtgtcgcgtt ttatgaagaa cacttcccgg atcgctattt tctcgagctg
    40141 atccgcaccg gcaggccgga tgaagaaagc tatctgcacg cggcggtgga actggcggaa
    40201 gcgcgcggtt tgcccgtcgt ggcgaccaac gacgtgcgct ttatcgacag cagcgacttt
    40261 gacgcacacg aaatccgcgt cgcgatccac gacggcttta ccctcgacga tcctaaacgc
    40321 ccgcgtaact attcgccgca gcaatatatg cgtagcgaag aggagatgtg tgagctgttt
    40381 gccgacatcc ccgaagccct tgccaacacc gttgagatcg ccaaacgctg taacgtaacc
    40441 gtgcgtcttg gtgaatactt cctgccgcag ttcccgaccg gggacatgag caccgaagat
    40501 tatctggtca agcgtgcaaa agagggcctg gaagagcgtc tggccttttt attccctgac
    40561 gaggaagaac gtgttaagcg ccgcccggaa tatgacgaac gtctggagac tgaacttcag
    40621 gttatcaacc agatgggctt cccgggctac ttcctcatcg ttatggaatt tatccagtgg
    40681 tcgaaagata acggcgtacc ggtagggcca ggccgtggct ccggtgcggg ttcactggtg
    40741 gcctacgcgc tgaaaatcac cgacctcgat ccgctggaat ttgacctgct gttcgaacgt
    40801 ttccttaacc cggaacgtgt ctccatgcct gacttcgacg ttgacttctg tatggagaaa
    40861 cgcgatcagg ttatcgagca cgtagcggac atgtacggtc gtgatgcggt atcgcagatc
    40921 atcaccttcg gtacaatggc ggcgaaagcg gtgatccgcg acgtaggccg cgtgctgggg
    40981 catccgtacg gctttgtcga tcgtatctcg aaactgatcc cgcccgatcc ggggatgacg
    41041 ctggcgaaag cgtttgaagc cgagccgcag ctgccggaaa tctacgaagc ggatgaagaa
    41101 gttaaggcgc tgatcgacat ggcgcgcaaa ctggaagggg tcacccgtaa cgccggtaag
    41161 cacgccggtg gggtggttat cgcaccgacc aaaattaccg attttgcgcc gctttattgc
    41221 gatgaagagg gcaaacatcc ggtcacccag tttgataaaa gcgacgttga atacgccgga
    41281 ctggtgaagt tcgacttcct tggtttgcgt acgctcacca tcatcaactg ggcgctggag
    41341 atgatcaaca agcggcgggc gaagaatggc gagccgccgc tggatatcgc cgcgatcccg
    41401 ctggatgaca agaaaagctt cgacatgctg caacgctcgg aaaccactgc ggtattccag
    41461 cttgaatcgc gcggcatgaa ggacctgatc aagcgtctgc aacctgactg cttcgaagat
    41521 atgatcgccc tggtggcact gttccgcccc ggtccgttgc aatcagggat ggtggataac
    41581 tttatcgacc gtaaacatgg tcgtgaagag atctcctatc cggacgtgca gtggcagcat
    41641 gaaagcttga aaccggtact ggagccaacc tacggcatca tcctgtatca ggaacaggtc
    41701 atgcagattg cccaggtgct ttctggttat accctcggtg gcgcggatat gctgcgtcgt
    41761 gcgatgggta agaaaaagcc ggaagagatg gctaagcagc gttctgtatt tgctgaaggt
    41821 gcagaaaaga acggaatcaa cgccgaactg gcgatgaaaa tcttcgacct ggtggagaaa
    41881 ttcgcgggtt acggatttaa caaatcgcac tctgcggcct atgctttggt gtcatatcaa
    41941 acgttatggc tgaaagcaca ctatcctgcg gagtttatgg cggcggtaat gaccgccgat
    42001 atggacaaca ccgagaaggt ggtgggcctg gtggatgagt gctggcggat ggggctgaaa
    42061 atcctgccac cagatataaa ctccggtctt taccatttcc acgtcaacga cgacggcgaa
    42121 atcgtgtatg gtattggcgc catcaaaggg gtaggtgaag gtccgattga ggccatcatc
    42181 gaagcccgta ataaaggcgg ctacttccgc gaactgtttg atctttgcgc ccgtaccgac
    42241 accaaaaagt taaaccggcg ggtgctggaa aaactgatca tgtccggggc gtttgaccgt
    42301 cttggaccac accgcgcggc actgatgaac tcgctgggcg atgcgttaaa agcggcagat
    42361 caacacgcga aagcggaagc tatcggtcag gccgatatgt tcggcgtgct ggccgaagag
    42421 ccggaacaaa ttgaacaatc ctacgccagc tgccaaccgt ggccggagca ggtggtatta
    42481 gatggggaac gtgaaacgtt aggcctgtac ctgaccggac accctatcaa ccagtattta
    42541 aaagagattg agcgttatgt cggaggcgta aggctgaaag acatgcaccc gacagaacgt
    42601 ggtaaagtca tcacggctgc ggggctcgtt gttgccgcgc gggttatggt caccaagcgc
    42661 ggcaatcgta tcggtatctg cacgctggat gaccgttccg ggcggctgga agtgatgttg
    42721 tttactgacg ccctggataa ataccagcaa ttgctggaaa aagaccgcat acttatcgtc
    42781 agcggacagg tcagctttga tgacttcagc ggtgggctta aaatgaccgc tcgcgaagtg
    42841 atggatattg acgaagcccg ggaaaaatat gctcgcgggc ttgctatctc gctgacggac
    42901 aggcaaattg atgaccagct tttaaaccga ctccgtcagt ctctggaacc ccaccgctct
    42961 gggacaattc cagtacatct ctactatcag agggcggatg cacgcgcgcg gttgcgtttt
    43021 ggcgcgacgt ggcgtgtctc tccgagcgat cgtttattaa acgatctccg tggcctcatt
    43081 ggttcggagc aggtggaact ggagtttgac taatacagga atactatgag tctgaatttc
    43141 cttgattttg aacagccgat tgcagagctg gaagcgaaaa tcgattctct gactgcggtt
    43201 agccgtcagg atgagaaact ggatattaac atcgatgaag aagtgcatcg tctgcgtgaa
    43261 aaaagcgtag aactgacgcg taaaatcttc gccgatctcg gtgcatggca gattgcgcaa
    43321 ctggcacgcc atccacagcg tccttatacc ctggattacg ttcgcctggc atttgatgaa
    43381 tttgacgaac tggctggcga ccgcgcgtat gcagacgata aagctatcgt cggtggtatc
    43441 gcccgtctcg atggtcgtcc ggtgatgatc attggtcatc aaaaaggtcg tgaaaccaaa
    43501 gaaaaaattc gccgtaactt tggtatgcca gcgccagaag gttaccgcaa agcactgcgt
    43561 ctgatgcaaa tggctgaacg ctttaagatg ccaatcatca cctttatcga caccccgggg
    43621 gcttatccgg gcgtgggcgc agaagagcgt ggtcagtctg aagccattgc acgcaacctg
    43681 cgtgaaatgt ctcgcctcgg cgtaccggta gtttgtacgg ttatcggtga aggtggttct
    43741 ggcggtgcgc tggcgattgg cgtgggcgat aaagtgaata tgctgcaata cagcacctat
    43801 tccgttatct cgccggaagg ttgtgcgtcc attctgtgga agagcgccga caaagcgccg
    43861 ctggcggctg aagcgatggg tatcatcgct ccgcgtctga aagagctgaa actgatcgac
    43921 tccatcatcc cggaaccgct gggcggcgct caccgtaacc cggaagcgat ggcggcatcg
    43981 ttgaaagcgc aactgctggc ggatctggcc gatctcgatg tgttaagcac tgaagattta
    44041 aaaaatcgtc gttatcagcg cctgatgagc tacggttacg cgtaattcgc aaaagttctg
    44101 aaaaagggtc acttcggtgg ccctttttta tcgccacggt ttgagcaggc tatgattaag
    44161 gaaggatttt ccaggaggaa cacatgaaca tcattgccat tatgggaccg catggcgtct
    44221 tttataaaga tgagcccatc aaagaactgg agtcggcgct ggtggcgcaa ggctttcaga
    44281 ttatctggcc acaaaacagc gttgatttgc tgaaatttat cgaacataac cctcgaattt
    44341 gcggcgtgat ttttgactgg gatgagtaca gtctcgattt atgcagcgat atcaatcaac
    44401 ttaatgaata tctcccgctt tatgccttca tcaacaccca ctcgacgatg gatgtcagcg
    44461 tgcaggatat gcggatggcg ctctggtttt ttgaatatgc gctggggcag gcggaagata
    44521 tcgccattcg tatgcgtcag tacaccgacg aatatcttga taacattaca ccgccgttca
    44581 cgaaagcctt gtttacctac gtcaaagagc ggaagtacac cttttgtacg ccggggcata
    44641 tgggcggcac cgcatatcaa aaaagcccgg ttggctgcct gttttatgat tttttcggcg
    44701 ggaataccct caaggctgat gtctctattt cggtcaccga gcttggttcg ttgctcgacc
    44761 acaccgggcc acacctggaa gcggaagagt acatcgcgcg gacttttggc gcggaacaga
    44821 gttatatcgt taccaacgga acatcgacgt cgaacaaaat tgtgggtatg tacgccgcgc
    44881 catccggcag tacgctgttg atcgaccgca attgtcataa atcgctggcg catctgttga
    44941 tgatgaacga tgtagtgcca gtctggctga aaccgacgcg taatgcgttg gggattcttg
    45001 gtgggatccc gcgccgtgaa tttactcgcg acagcatcga agagaaagtc gctgccacca
    45061 cgcaggcaca atggccggtt catgcggtga ttaccaactc cacctatgat ggcttgctct
    45121 acaacaccga ctggatcaaa cagacgctgg atgtcccgtc gatccacttc gattccgcct
    45181 gggtgccgta cacccatttt catccgatct accaggggaa aagtggtatg agcggcgagc
    45241 gtgttgcggg aaaagtgatc ttcgaaacgc aatcgaccca caaaatgctg gcggcgttat
    45301 cgcaggcttc gctgatccac attaaaggcg agtatgacga agaggcgttt aacgaagcct
    45361 ttatgatgca taccaccacc tcgcccagtt atcccattgt tgcttcggtt gagacggcgg
    45421 cggcgatgct gcgtggtaat ccgggcaaac ggctgattaa ccgttcggtg gagcgggcgc
    45481 tgcattttcg caaagaggtc cagcggttgc gggaagagtc tgacggttgg tttttcgata
    45541 tctggcaacc gccgcaggtg gatgaagccg aatgctggcc cgttgcgcct ggcgaacagt
    45601 ggcacggctt taacgatgcg gatgccgatc atatgtttct cgatccggtt aaagtcacta
    45661 ttttgacacc ggggatggac gaacagggca atatgagcga ggaggggatc ccggcggcgc
    45721 tggtggcaaa attcctcgac gaacgtggga tcgtagtaga gaaaaccggc ccttataacc
    45781 tgctgtttct ctttagtatt ggtatcgata aaaccaaagc aatgggatta ttgcgtgggt
    45841 tgacggaatt taaacgctct tacgatctca acctgcggat caaaaatatg ctacccgatc
    45901 tctatgctga agatcccgat ttctaccgca atatgcgtat ccaggatctg gcgcaaggga
    45961 tccataagct gattcgtaaa cacgatcttc ccggtttgat gttgcgggca ttcgatactt
    46021 tgccggaaat gatcatgacg ccacatcagg catggcagag acagattaaa ggtgaagtag
    46081 aaaccattgc gctggaacaa ctggtcggta gagtatcggc aaatatgatc ctgccttatc
    46141 caccgggcgt accgctgttg atgccaggag aaatgctgac caaagagagc cgcacggtac
    46201 tcgattttct actgatgctt tgttccgtag ggcaacatta ccccggtttt gaaacggata
    46261 ttcacggcgc gaaacaggac gaagacggcg tttaccgcgt acgagtccta aaaatggcgg
    46321 gataacttgc cagagcggct tccgggcgag taacgtgctg ttaacaaata aaggagacgt
    46381 tatgctgggt ttaaaacagg ttcaccatat tgcgattatt gcgacggatt atgcggtgag
    46441 caaagctttc tactgcgata ttcttggttt cacgctgcaa agcgaagtct atcgcgaagc
    46501 gcgcgactcg tggaaagggg atttggcgct taatgggcaa tatgtgattg agcttttctc
    46561 ttttccgttc ccgccggagc gccccagccg accggaagct tgcggcctgc gtcatctggc
    46621 ttttagcgtt gatgacatcg atgcggcagt ggcgcacctt gaaagccata acgtgaagtg
    46681 tgaagccatc cgtgtcgatc catacacgca aaaacgcttc accttcttta acgatccgga
    46741 cgggctgccg ttggaactgt atgagcagta aggcttgtca tcgccgcatt tgcccggtaa
    46801 cgtgccgggc attgctactg taaaatcgca ccatcatgac actcacgctc aatagacaac
    46861 ttctcacctc acgccagatt ctggtggcct ttagcggcgg gcttgactcc accgttctgc
    46921 tgcatcagtt ggtgcagtgg cggacggaaa atccgggcgt cactctgcgc gctattcatg
    46981 tgcatcacgg tttaagtgcc aatgccgatg cctgggttac gcattgcgaa aacgtctgcc
    47041 aacagtggca ggtgccgctg gtggtcgaac gcgtacaact tgcgcaagag gggctgggga
    47101 ttgaggccca ggcgcggcag gcgcgttatc aggcatttgc ccgtacccta ttgcccggcg
    47161 aagtgctggt caccgcgcaa catctcgacg atcaatgtga aacctttctg ctggcgctaa
    47221 aacgcggtag cggccctgcc gggctttcgg ctatggggga agtctcggag tttgccggaa
    47281 cacggcttat ccgcccgttg cttgcccgca cgcgggggga actggcgcag tgggcattgg
    47341 cgcatggttt acgctggatt gaagacgaaa gtaatcagga cgactcatac gatcgtaact
    47401 ttctgcgcct gcgtgtagtg ccgttattgc agcagcgttg gccgcatttt gccgaagcaa
    47461 cggcccgcag cgccgcactt tgtgctgaac aagagagtct gttggatgaa ctgctggcgg
    47521 atgatttagc acactgccaa acgtcgcagg ggacgctgca gattgcgcca atgctagcga
    47581 tgagtgatgc ccgtcgcgcg gcgattatcc gccgctggct ggcagggcag aatgcaccga
    47641 tgccttcccg cgacgcgctg gtgcggatct ggcaggaagt ggcgctggcg cgggaagatg
    47701 cctccccctg tttacgcttg ggcgcgtttg aaatccgccg atatcagtcg caactgtggt
    47761 ggattaaatc cgtcaccggg caaagtgaaa ccattgtgcc gtggcagacg tggctacaac
    47821 cgctggaatt accggcgggg ctgggaagcg tacagcttac tgcgggaggc gatattcgcc
    47881 ctccgcgtgc agacgaagcg gtcagcgtgc gtttcaaagc ggcaggattg ctgcatattg
    47941 tcgggcgcaa cggcggacgt aagctgaaga aaatctggca agagttaggc gtgccgccgt
    48001 ggctacgtga caccacgcca ctgctgtttt atggcgaaac gctgattgcg gcggcagggg
    48061 tatttgtgac gcaagaaggt gtggctgaag gtgagaatgg cgtaagtttt gtctggcaga
    48121 aaacgcttag ttaagtgaaa gccggataag acgcatcaaa cgtcgcatcc ggcgaaagtc
    48181 aatcaggact cgctcaccac caccgtaccg atttccgggt ggctaaagct ggtaatttta
    48241 tccagacgta gctcacgggt ttcgccagcg gcctcgacga ccagatactc cacatttttg
    48301 cgggagacta aatcgctggc ttttgcctgc aatttttcgc catctttcag ctcaagtgtc
    48361 agcattaaat gatgctggca ggcgagctca agattatcgt aatcatcaca attgattggt
    48421 tgatacgtat cattcattga cataatcgct caccagtaag tttgccgcag cgtatgctgc
    48481 tttttccctg acagcctcag aaagggcgtc gtcggcagcc atttcattca gcactttcaa
    48541 aacgcagccc agcgcgtccg gaacgtatcc taagtctccg ctggcgattt ccgcgtaccg
    48601 cttgcgtatt aactcacaat atttttccac atgccctcct gtcagcactc tgacttaacc
    48661 gtggatgcaa gtctaagcct acgaagataa actctgtttc gcaaggtgac tataccacac
    48721 tcatttctgc aatatcagcg tcgcaactgc acgtattccg ttacaatggc ctcctgattc
    48781 gaaaggagtt ttcttatggc gcttaaagcg acaatttata aagcgacggt taatgtggcc
    48841 gatctcgacc gcaaccagtt tctcgatgcc tctctgacgc tggcgcgcca cccttcagaa
    48901 acccaggagc gtatgatgct gcgcttgctg gcgtggctga aatatgctga tgaacgtctg
    48961 caatttaccc gtggtttgtg tgccgatgat gagccggaag cgtggctgcg taacgatcac
    49021 atgggcattg atttgtggat tgagctgggg ctgccggatg agcggcggat taagaaagcc
    49081 tgcacccagg ccgcagaagt ggcgctgttt acctataata gtcgggcggc gcaaatctgg
    49141 tggcagcaaa atcagagcaa atgtgtgcag tttgccaatc tttccgtctg gtatctggac
    49201 gatgaacaac tggcgaaagt aagcgccttt gccgatcgta ccatgacgct gcaggcaacg
    49261 attcaggatg gcgtgatctg gttatcggac gataagaata atctggaagt taacttaacc
    49321 gcctggcaac aaccttcatg attgtgattt cccgacatgt tgctattccc gatggtgagc
    49381 ttgagatcac cgccattcgt gcgcagggcg cgggcgggca gcatgttaat aagacctcaa
    49441 cggccattca tctgcgtttt gacattcggg cgtccagcct gccagagtat tacaaagagc
    49501 gtctactcgc cgacagccat catttgatca gcagtgatgg cgtgattgtc attaaggcac
    49561 aggaataccg cagtcaggaa ctgaaccgcg aagccgcgct agcccggctg gtggcagtga
    49621 ttaaagattt aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga
    49681 aagagcgcag gctggcatcg aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag
    49741 tacgcagcgg tcgggaataa aaagaaggaa tagatggtga aaaaagcgat agtgacagcg
    49801 atggctgtaa tcagcctctt tactctgatg ggatgtaata accgggccga agtcgatacg
    49861 ctttctccag cacaggctgc cgaactgaaa ccgatgccgc aaagttggcg cggcgtgctg
    49921 ccgtgtgctg attgcgaagg aatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg
    49981 gtgatgaatg agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt
    50041 acatgggcgc gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat
    50101 tatcgggcga aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc gattgaatcg
    50161 cagttcaact atacgctgga accggcacaa tccagtttac ccctgacgcc gatgaccctg
    50221 cggggcatgt atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa
    50281 cgtttcatgg tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac
    50341 agtgaaaaac cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat
    50401 actggtgcgc caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag
    50461 gattgcagta gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg
    50521 atgcgct
//
LOCUS       DAZGXZ010000017        48391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000017 DAZGXZ010000000
VERSION     DAZGXZ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.017"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHKJF_001128"
     CDS             complement(<1..296)
                     /locus_tag="ACHKJF_001128"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0948528.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESP"
     gene            complement(323..871)
                     /gene="wcaF"
                     /locus_tag="ACHKJF_001129"
     CDS             complement(323..871)
                     /gene="wcaF"
                     /locus_tag="ACHKJF_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP0948529.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATLFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(887..1633)
                     /gene="wcaE"
                     /locus_tag="ACHKJF_001130"
     CDS             complement(887..1633)
                     /gene="wcaE"
                     /locus_tag="ACHKJF_001130"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP0948530.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNSIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            complement(1644..2861)
                     /gene="wcaD"
                     /locus_tag="ACHKJF_001131"
     CDS             complement(1644..2861)
                     /gene="wcaD"
                     /locus_tag="ACHKJF_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP0948531.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGVGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(2836..4053)
                     /gene="wcaC"
                     /locus_tag="ACHKJF_001132"
     CDS             complement(2836..4053)
                     /gene="wcaC"
                     /locus_tag="ACHKJF_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP0948532.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(4050..4538)
                     /gene="wcaB"
                     /locus_tag="ACHKJF_001133"
     CDS             complement(4050..4538)
                     /gene="wcaB"
                     /locus_tag="ACHKJF_001133"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP0948533.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     AENMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(4541..5380)
                     /gene="wcaA"
                     /locus_tag="ACHKJF_001134"
     CDS             complement(4541..5380)
                     /gene="wcaA"
                     /locus_tag="ACHKJF_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP0948534.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(5558..7720)
                     /gene="wzc"
                     /locus_tag="ACHKJF_001135"
     CDS             complement(5558..7720)
                     /gene="wzc"
                     /locus_tag="ACHKJF_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP0948535.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(7723..8166)
                     /gene="wzb"
                     /locus_tag="ACHKJF_001136"
     CDS             complement(7723..8166)
                     /gene="wzb"
                     /locus_tag="ACHKJF_001136"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP0948536.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(8172..9311)
                     /gene="wza"
                     /locus_tag="ACHKJF_001137"
     CDS             complement(8172..9311)
                     /gene="wza"
                     /locus_tag="ACHKJF_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP0948537.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            9620..9769
                     /locus_tag="ACHKJF_001138"
     CDS             9620..9769
                     /locus_tag="ACHKJF_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948538.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYINCHSIWFL
                     GVSLG"
     gene            9970..11553
                     /gene="yegH"
                     /locus_tag="ACHKJF_001139"
     CDS             9970..11553
                     /gene="yegH"
                     /locus_tag="ACHKJF_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0948539.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEVEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(11827..13680)
                     /gene="asmA"
                     /locus_tag="ACHKJF_001140"
     CDS             complement(11827..13680)
                     /gene="asmA"
                     /locus_tag="ACHKJF_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP0948540.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVKMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(13702..14283)
                     /gene="dcd"
                     /locus_tag="ACHKJF_001141"
     CDS             complement(13702..14283)
                     /gene="dcd"
                     /locus_tag="ACHKJF_001141"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP0948541.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(14375..15016)
                     /gene="udk"
                     /locus_tag="ACHKJF_001142"
     CDS             complement(14375..15016)
                     /gene="udk"
                     /locus_tag="ACHKJF_001142"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP0948542.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            15334..18651
                     /gene="dgcE"
                     /locus_tag="ACHKJF_001143"
     CDS             15334..18651
                     /gene="dgcE"
                     /locus_tag="ACHKJF_001143"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0948543.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(18690..19547)
                     /gene="alkA"
                     /locus_tag="ACHKJF_001144"
     CDS             complement(18690..19547)
                     /gene="alkA"
                     /locus_tag="ACHKJF_001144"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP0948544.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            19681..21033
                     /gene="yegD"
                     /locus_tag="ACHKJF_001145"
     CDS             19681..21033
                     /gene="yegD"
                     /locus_tag="ACHKJF_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0948545.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(21045..22991)
                     /gene="yegI"
                     /locus_tag="ACHKJF_001146"
     CDS             complement(21045..22991)
                     /gene="yegI"
                     /locus_tag="ACHKJF_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP0948546.1"
                     /translation="MKTNIKVFTSTGEMTTLGLELGKGGEGAVYDINEFVDSVAKIYH
                     TPPPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMI
                     YSPAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLI
                     DSDSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTKNHDNFGLALLIFHVLFG
                     GRHPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMF
                     QQAFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGV
                     IYFIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRRE
                     YIILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEIQQRREAFN
                     RAKMDYDHLVSQSQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQ
                     KQKFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVID
                     WKASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPART
                     MPLMEPLRQAAEKLAQAQADLSRC"
     gene            23189..23650
                     /gene="yegJ"
                     /locus_tag="ACHKJF_001147"
     CDS             23189..23650
                     /gene="yegJ"
                     /locus_tag="ACHKJF_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFP0948547.1"
                     /translation="MKKILLMLSLLFFTTAGFSEVSDTLVTGGYDKQAMSDAIKHARK
                     ETDKFIEVMNKKDADTFAVKAPITDHGRTEHFWLTDVTYSNGMFIGVISNDPGIVTNV
                     EYGQEWKIKKEDISDWMYTRGDKIYGGYTIDPLLVTYPKEEADELRAKLVR"
     gene            complement(23715..24476)
                     /gene="pphC"
                     /locus_tag="ACHKJF_001148"
     CDS             complement(23715..24476)
                     /gene="pphC"
                     /locus_tag="ACHKJF_001148"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFP0948548.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWAE"
     gene            complement(24473..25132)
                     /gene="yegL"
                     /locus_tag="ACHKJF_001149"
     CDS             complement(24473..25132)
                     /gene="yegL"
                     /locus_tag="ACHKJF_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0948549.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(25353..25412)
                     /gene="ibsA"
                     /locus_tag="ACHKJF_001150"
     CDS             complement(25353..25412)
                     /gene="ibsA"
                     /locus_tag="ACHKJF_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP0948550.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(25685..25741)
                     /locus_tag="ACHKJF_001151"
     CDS             complement(25685..25741)
                     /locus_tag="ACHKJF_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0948551.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(26013..26069)
                     /locus_tag="ACHKJF_001152"
     CDS             complement(26013..26069)
                     /locus_tag="ACHKJF_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0948552.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            26348..27595
                     /gene="mdtA"
                     /locus_tag="ACHKJF_001153"
     CDS             26348..27595
                     /gene="mdtA"
                     /locus_tag="ACHKJF_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP0948553.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSTTTSEEKATSREYAKKGARS"
     gene            27595..30717
                     /gene="mdtB"
                     /locus_tag="ACHKJF_001154"
     CDS             27595..30717
                     /gene="mdtB"
                     /locus_tag="ACHKJF_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP0948554.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            30718..33795
                     /gene="mdtC"
                     /locus_tag="ACHKJF_001155"
     CDS             30718..33795
                     /gene="mdtC"
                     /locus_tag="ACHKJF_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP0948555.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSIQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIILVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPCEQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANNLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            33796..35211
                     /gene="mdtD"
                     /locus_tag="ACHKJF_001156"
     CDS             33796..35211
                     /gene="mdtD"
                     /locus_tag="ACHKJF_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0948556.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNHRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            35208..36611
                     /gene="baeS"
                     /locus_tag="ACHKJF_001157"
     CDS             35208..36611
                     /gene="baeS"
                     /locus_tag="ACHKJF_001157"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP0948557.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            36608..37330
                     /gene="baeR"
                     /locus_tag="ACHKJF_001158"
     CDS             36608..37330
                     /gene="baeR"
                     /locus_tag="ACHKJF_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP0948558.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRVKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            37521..37853
                     /gene="yegP"
                     /locus_tag="ACHKJF_001159"
     CDS             37521..37853
                     /gene="yegP"
                     /locus_tag="ACHKJF_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP0948559.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            38062..38358
                     /locus_tag="ACHKJF_001160"
     CDS             38062..38358
                     /locus_tag="ACHKJF_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0948560.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            38360..38656
                     /locus_tag="ACHKJF_001161"
     CDS             38360..38656
                     /locus_tag="ACHKJF_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0948561.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            38759..40120
                     /gene="yegQ"
                     /locus_tag="ACHKJF_001162"
     CDS             38759..40120
                     /gene="yegQ"
                     /locus_tag="ACHKJF_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP0948562.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(40450..40767)
                     /gene="yegR"
                     /locus_tag="ACHKJF_001163"
     CDS             complement(40450..40767)
                     /gene="yegR"
                     /locus_tag="ACHKJF_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP0948563.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            41173..42072
                     /gene="yegS"
                     /locus_tag="ACHKJF_001164"
     CDS             41173..42072
                     /gene="yegS"
                     /locus_tag="ACHKJF_001164"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP0948564.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVATALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(42154..42927)
                     /locus_tag="ACHKJF_001165"
     CDS             complement(42154..42927)
                     /locus_tag="ACHKJF_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0948565.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(43033..44073)
                     /gene="gatD"
                     /locus_tag="ACHKJF_001166"
     CDS             complement(43033..44073)
                     /gene="gatD"
                     /locus_tag="ACHKJF_001166"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0948566.1"
                     /translation="MKSVVNDTDGVVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVNRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQGVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(44121..45476)
                     /locus_tag="ACHKJF_001167"
     CDS             complement(44121..45476)
                     /locus_tag="ACHKJF_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP0948567.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDTIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            complement(45480..45764)
                     /gene="gatB"
                     /locus_tag="ACHKJF_001168"
     CDS             complement(45480..45764)
                     /gene="gatB"
                     /locus_tag="ACHKJF_001168"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP0948568.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(45795..46247)
                     /gene="gatA"
                     /locus_tag="ACHKJF_001169"
     CDS             complement(45795..46247)
                     /gene="gatA"
                     /locus_tag="ACHKJF_001169"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP0948569.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(46257..47519)
                     /gene="gatZ"
                     /locus_tag="ACHKJF_001170"
     CDS             complement(46257..47519)
                     /gene="gatZ"
                     /locus_tag="ACHKJF_001170"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP0948570.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(47548..>48391)
                     /gene="gatY"
                     /locus_tag="ACHKJF_001171"
     CDS             complement(47548..>48391)
                     /gene="gatY"
                     /locus_tag="ACHKJF_001171"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP0948571.1"
                     /translation="STKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVIIA
                     GTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMIDAS
                     HLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQAREFA
                     EATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTI
                     KVGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGC
                     EGRA"
BASE COUNT        11640 a        12689 c        12189 g        11873 t
ORIGIN      
        1 ggtgactcaa aagaaaccgc aacgtggctc attgccccca ggttgtacac ttcatccggc
       61 tgtacttcac gcaaaatgcg cgtcaggttg gaggtatcac tcaggtcgcc ataatgcaga
      121 tggaattttg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg
      181 aatgacgatg cacgacgctt aataccatgc acctcgtaac ctttttccag cagaaactct
      241 gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt tgacatgtct
      301 tattcctctg tattttttga atttattcag tttcaacgcg ttcgcgtatc accactgcgg
      361 gattcccccg gcaaaccaca tttgccggaa gcgatttaaa aacactgctt cgcgccccca
      421 cgacggtgcc gtcgccgatt gtgacgcctg gggcaacaaa gacatcggtt gccagccagc
      481 atttctcgcc aatcacaata ggcgtggcgt taatggtgaa atgttgactt gcatggtcgt
      541 ggctaccggt gcataaataa cttttttgcg atatcaccga atgtgcgcca atggttattt
      601 caccgagggt atataaattg acctcatcgc cgacccacgc gtaatcacct aaggttaatt
      661 tccacggata ggtaattttt actgacggac gaataactac gtttttacct atttttgccc
      721 cgaataaacg taataaaaat gcccgccagc gatacaatac ttgtggcgac caggcaaata
      781 atgttgcctg tactgcccac cacaattgca ctttaatagc gttgccgccc cggaaccctt
      841 tcggcaccga gaaaccgctt aaatcttgca tcgttttccc ttatattcag cttttgttat
      901 ataaggcttt cgtctttgag gtagtacgtt ggcgtaaatg ccaggataat tctgcccaga
      961 agccaggcac atgtaatatt tgccgttgga cttttttggc gtcagcacac aattccatat
     1021 tattggtggt agatacccca cccatagaaa attcagacac caggccattg agttttttaa
     1081 atgcataacc tgctttatac attttggctg ccagcgcgta atcagaagaa actttatatt
     1141 ccaggtcata acgccatttt ttcaagccgg ataccgggaa aaatatcgcc tgatggctgg
     1201 cgggcaggct gtgataaata taccaacccg gtttggcgct acgtttaatt ttatgcccgt
     1261 cgccaaaatc cagcagcgca tcgccggtga tcatcatgtt atctttttgc atttttaact
     1321 tacggacaaa atttgcggca tcctgatgaa aaatatcgcc cgaattgaga aacaacgcga
     1381 acttgccttg tgccatcgca atgcccttgt tcatggcgtc gtagataccg ttatctggct
     1441 cgctgacaaa gcgtaggtta tagatactat tgagattttc cagatactca cgggtaccgt
     1501 cgttggaacc gccatcgaca acaatccatt cgaagctgat atcgtccgcc tgcgccagat
     1561 gcgccagcga ggcatgtgtt ttgactatcc cttcgaggtt acgaaacgcg acagtgatta
     1621 tgctaagcaa catgttctta ttcttacctc gtaatattta acgcttttcg caaaataaac
     1681 ggacagacga ttaaaaatgc atattccggg ctaaatatcg aaccggtaaa aaacagcgat
     1741 accggagtaa aaagatataa ttgcacgcga aaattgcggt tatcaccaaa agcgttgatc
     1801 atcattttta tcactttccc catgtaccac agcgataaaa acaccgcgaa ccaggaaaaa
     1861 taaataatca gcagatacaa accattgtct atggtttttc cgacatccgc accgttaaat
     1921 attccgaatg atgcgacata ttcataaagt gagccaaatc tgactacacc gtcaatatgg
     1981 gtcaaagaat aaccgaccat caccagcgga ccgacaatac gataatacga agacgatcct
     2041 tccgtaccta aatcgcccag acgggtggaa atataaggaa acgcgattac cacaccaacc
     2101 aggaatacag ccagagaaat caatgctaac ggtaactttt tcttaatcgc ctctttattc
     2161 agatattgga atgcccactc cagcaaataa aacaggataa aggtcataac ccctgaaaac
     2221 gatccggata atattatccc tgcgagaatc atagcatctg ttttaggcgt tttgatacca
     2281 aactgtttga tgctgagcca aattgagatt aatgccagag cgaaaaatgc cggttcgaaa
     2341 taaagtgctg tcgtgcgctt gccgccgaat ttaatgaaat tcagtacata gctgttgcta
     2401 taaatcagat atttcgaaat cgactccata atactgctgc caccggtgag gataatttgc
     2461 gccatctcca ccgccgccag cgccaccacc agcccgacca ccagataaaa gaaacgtaat
     2521 atcttgcgat ggttgtgtgg cgaaatcgtt ttaaagcgaa tactccacac catgccaata
     2581 atgatcacaa tatagacaaa cagcatggtt gaagtgacgt atttactggc atccaacgac
     2641 tgaccaaaca gatagttgaa cgccgtgagc cccgcgccaa cccctaaggc aatcatcaat
     2701 tttttaacgc tgatgcgttc taaaaacagc agtaacaaga caggtaaaaa agtgacgata
     2761 gtgatgggga aactttcacc aagctgggcg attttgacgt taaccagcag gtagatcagc
     2821 ggcagcagca ggtagctaca gattctgata gaagttgaca tactcctcca gcatctgttg
     2881 tccactgtag gcggcgcggc tacgttggct gaacccggcc aacgtggtac caaatatcgc
     2941 ctgcgcgatt tccggtttgc ttaattgcac cagttgcagc acctcttctt cgctgacggt
     3001 tttaccgccg gatttttgca acacttcacg cgccgcgtcg ctatgggtgg caatcaccgg
     3061 cacgccaatc gatagcgcct cacacaaaat cagcgggtag ttatcgacgc gagaactgaa
     3121 caccagcgca tccatctgat tgagcgcgct catcaacttg cgcttgtcag tttcaaagcc
     3181 gtgattaacc acgttgccag cggtgaacgg cgagaactta ccaaaggtat gcagttcaat
     3241 tttgtcgccc agcgccatca tctcacgcac cagttgctgg ttagtcttgc cgtcgtaacg
     3301 caggtcatgc gccaccaccg cgattttcgg cttaccctgg gtttcgcgca ccggaggcag
     3361 atccgccaga atcgcttcgg ttgccatatc aataccgtta ttgataatcc ggcaacgccc
     3421 tggaccgtac aggctattga aagcgtcagc cacatgctgg ctgggtgaaa taaactgaca
     3481 gcccagcgcc agcatctcac ggaataactg gcgtttgccc gccaccagct ggtgcgcccg
     3541 atcaatcttc accggcggat aattatttaa ggtcgggcat ttctggcagc ccgttttcca
     3601 gccttcacaa ccgtcggtaa aggcgcagcg cccggtaacg ctccagtggt catgcagcgt
     3661 ccagaccaga gtgacatccg gtttgtggtt tttcaccttt tcgcaaaagc gcaccacgct
     3721 cttaagattt agccagtagc tgtgcagcac atgaaaatgc aggaccaccg gacccggtgt
     3781 gcgagtaatg gtgcgatata actcattgaa attgccaaac agatcgcgat taaacagacg
     3841 aaacagggca atattcgcca ttgcggtcat ccgcggcgta tgtttgatta cctgcggata
     3901 gttctgatgg ctgacgctct ctttgccgcc tttgccgtag ccgtagacaa aatgtgacgc
     3961 cagcccctgt tgcagcgcac gctggtggag atctaacgcc acacctgccg ccccgccttc
     4021 cgccagtcgc acattaaatt gcagaatatt catttaatta ccttcactcg cgctttttct
     4081 cccaccacca gcgcgttgtc cgggacagag tcgagcacca cgctgcccgc gcccacggtg
     4141 acgttgttac caagcgtgat atcaccaaga ataatgacgt tggcaccgag ttcgacgccg
     4201 ttgccaatgt gtggacatgc catgttttcg gcaccacgat tgccgatagt gacgccgtgg
     4261 cgaatggtaa aatcatcccc cgctactacg tttttattga tcactacggc gtaaccgtga
     4321 tggatagtaa agcggcggcc aatggtcgcg gcggcctgga tttcataacc gaaaaagcat
     4381 tcggtgataa tgcgatacag caccagcagc ggggccgccc acagattgtt gaggacgttc
     4441 tttttgcgcc acaccgaaca aaaatgagca acacgatagg caagaaccat gcagcacggg
     4501 cgtaaactcc agctgttggc acgcagatct tccagcatct ttagcgcccc cgaataccat
     4561 cagccagacg tttgccgtta cgcaccgaca gcagcgtcag caaagtgcgc caggtcatac
     4621 gtttattgcg gatctggtag agggtaaaca gctgatattt tttgcttgca cggtcaaatt
     4681 tatctttgtg cttgcggtaa aagtggaagt acccggagaa ttttttcggt gacgaggtga
     4741 tctgcatttc accgtgattg atatgcagga tctgcgtcgc ctcttccact ttccacggtt
     4801 cgccatactc caccaccatc cgcaggaaga tgtcgtaatc ctgtgctgct ttcagttcgg
     4861 tatcgaacaa gcactctttg aaacgccacg cccaggtaaa aacctgattg ccaatgatat
     4921 tgcgtttata gaacaggcgg cgtgagtacg gcgatttggg atacagtggt aagcttgccg
     4981 gttgggaata aacttctccc tggcatacgt agtcgttagc gtacaaaaag gcgtgagtga
     5041 ccagttgctg tttatgggcg aggaagacgc tcaggcggtt gggcgtccat tcatcatcgt
     5101 catcgatccc ggtaatgtat tccccttgcg ccagcataat cgcctggtta cgtaccgcgc
     5161 acgccccgct gttaatgtcg ttgtgaatgt aggtaatacg cggatcgttg agggcagtga
     5221 cgtactgttg cagttgttcc caggaggtgg agcaatcatc cacgatgatc atctcccagt
     5281 tgctgtagtc ctggcgcagg accgatttta tcgcccgaat cgccagttgt tggcggttcc
     5341 aggtcggcat atagattgaa atcagcggat tgtctttcat aggttgttct cccggagtgt
     5401 cgagatgttc tgccggttta taagcttgtt tacgggttct gttgccggat gcggcgtgag
     5461 cgccttatcc ggcctacgga gctggtttgc ttgccggatg cggcgtgaag gtaggtcgga
     5521 taagatgcgt cagcatcgca tccgacagcg gatgtcgtta tttcgcatcc gacttatatt
     5581 cgtattcgta atagccataa tcctgatacg cgctggcgcg gcggaagatg gagttcagaa
     5641 tcaccccttt caccggaata ccattttgct caaagcggct cagactggtt tccacttctt
     5701 tcaatgtgtt gaccgcataa cgcgccacca ttaacgtggt tccaacatga cgaccaacaa
     5761 ttgccgcatc ggtcactgcc agaatcggcg gcgtatcaat caacaccagg tcatagtttt
     5821 tactcgccca gttcaccagt tcggcaaagc gttcgctcat caacagttca gaaggatttg
     5881 gcggtacctg accgcgcggg atcaggtcaa atttggcaat agaggtcggt ttagctgcgg
     5941 tagtaatatc gccctggcca atcagaattt ccgacaggcc attgacgtta ttggtaccca
     6001 acagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat caacaacacc cgtttattgg
     6061 tctgactgat caccgccgcc aggttggcgc agacaaaggt tttaccgatt gacgggctca
     6121 cccctgtcat catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc agactggtac
     6181 gcagactgcg gatggcttca atcgccagat cggttggatt ccccaccgcc agtagctggc
     6241 tctgtttata gcgtttaatc cctttgatgg ttttgacgct atcgcgcgct ttctgccatt
     6301 ccgacaacgg gatgctggca tagacgctga taccatgttc ttccagcacc tgcgggcttt
     6361 cgatgccgcg attaaacaac gagcgcagca gcacacccac gatagagagc atcaggccga
     6421 ggataattgc gccgaggata atcagtcctt tcttcggttt cagcacgcca ggctgggtga
     6481 ttgccgggtc aacaatgcgc acatcgccga cggtgctggc ttcggtgatt ttcagctcct
     6541 gctctttatt cagcagttgc atatagacct gctgaccaga ctcgacatcg cgggtcagac
     6601 ggacaatttc ctgctgggtt ttcggcatcg ccgttacgcg accattaagt ttggcttttt
     6661 cgtcttccag cgcctgacgt ttctccagca gcgtgcggta cgcggggtga actttggtgt
     6721 acagcttgga gatttccgcc tctttaaagg tcagttcgtt caactgcgcg tcgatgttca
     6781 ccatcgaatc gagcaccgct ttcgcttcca gcggcagatc aacagaatct ttatcctgac
     6841 ggaaggcatt cagtttgttt tcggcaacat cgaggcggct acgtacttcc ggtaactgtt
     6901 gcgcgaggaa agcgaggctt ttcgacgctt ccgccgattt gcgctcaata ttttgttcct
     6961 gatagttacg ggcgatgctg ttaagaatgt cgcggatctg ttcgcgatct tcaccggtat
     7021 aagtcaggct cagtacgcct gcgtctttgc cgttctccgt taccgtcagg ctgttttgca
     7081 gttggttgat catccccaac gtggagtatt tggtgacggt aaactcactg cccgggcggg
     7141 cgtgaatggc ttcaaccatc agcgtgacgc cttctttttt cagcatctgg cccgcttgcc
     7201 cacgggcgct aaagccgcca tcactgctca gagtgtagtt tttgtcatcc agcacattaa
     7261 gcgtaaacac ctggtccgcc atctctttcg ggcggttaaa ggtggtcact ttcaccgtct
     7321 cgttctgacg tcccatcagg cgatcccaac ccgcaccgaa aatcgggaac gtgtttttgc
     7381 tcaccgcaat atcgaggtct agatcgtcca ccgttttacc aagcaccaga cgcgaacgaa
     7441 tcaactggat ctcggcgtcc gatgcaggcg gtttgttggc taatgccgag ccaatgtcct
     7501 gcactaacga attgccgctg ttttgttcga tttgtaccag tgcgtcggca ctataaatcg
     7561 gcgtggcgaa gaaagtgtaa accacagcac agagggcaaa tacagcggtg atgccaatca
     7621 cccaccagcg cgcttcaatg acggtgccga ccaggcgacc aatatcgatt tcatcactgc
     7681 ccgttaccgg agcggcatgt tgttttactt tttctgtcat tcttatacct gctctgcgtt
     7741 caatgcctgc gcccactggc gggcagaccg ttcaagtaat gtgtacaccg ctgcaaacgt
     7801 ttcccggctt ttgcgatacg gatcggggat ttcacattcg ttatcccagt gaccaaacag
     7861 catcactttg ccgcgcatct caggtgccat ctcgcataag cgttcgatat ggcgcttttc
     7921 catggtcaaa atcaggtcat agttgcgaca caggcggcgg ctgatttgcc gtgcacagtg
     7981 accttccaga gacagttgat gttctgcggc gacgctgata gcggtaggat cagcgccctt
     8041 accgaccagc gcgccgagtc cagcggactc cactttcagc tccgggtgat aacgttgcag
     8101 taagcgttcc gccgtcgggg aacggcaaat attgccgaca cagacaacta agatgttgtt
     8161 aaacatgacg attaccagtt atgaatgtcg ctggctgtat ccgtcatgta acggacaccg
     8221 ctaatagttg gcagcaactg attgatcaga cggttccagc gggaaaccgg ggcggtggta
     8281 acatacacca cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag cgacgtcgca
     8341 tcggacatat ccagctggta gatattggca atcttgccat tacgcccgcc ctcgcctttc
     8401 agcggacgaa tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat accttcggca
     8461 ttgcccaggg cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt gctctgtttc
     8521 ttcacttcac ccatcacaaa cactttcaga tcatcattac gcggcacgta gagaatatcg
     8581 ccgggataaa gcagtcggtt ctgattgagg tcgccgtttt gcatcagcgc ctgcaaagaa
     8641 atgcgttctt cacgaccatt gtgtgttagc accacgttgc gccagtcagc ggtgtcggtc
     8701 aggccacctg cggcgttgat ggcgtcgaga atggtcagtg gtacgttggt gatcgcctgt
     8761 tgaccggatt tattcacctg tccggagata taggcctttt gtgagcggaa ggcggcgata
     8821 ttaacgtcca cctgcgggtc agcgatgtac gtcgctaagc gcccggtaat atcactgcgg
     8881 atttcagcga gcgttttccc gactacgtga accttgccga tatacgggta aaacatagtg
     8941 ccgtcaggct gtacccagtt gccggtgtca ctggagctgc ggtactgacc ggctggcgtg
     9001 gtgagttccg ggtgatccca tacggtgaca ttcagaacgt cgcccggccc gacgcgatac
     9061 tggtaattcg cgatctcact ttccagcgtc atattggggc gcgctacgtt gggtcgtgga
     9121 cgtaattgat caatcaggcg cggggtcaac ggataaacat tcaccatttt gtcgagatcg
     9181 aaatcagcgt cctgctgttt gatgacgtct ttgcccatcg tcgacatatt gctgcccgga
     9241 agtactgtgc aaccgcttat caaggttact gacaccaata atggcatcaa tttcattttg
     9301 gatttcatca ttgtttattt atcactttgg cagagtaatt atcctgtgca ctattaatag
     9361 caatgtcgcc atgcacattt accttgcagt taattgaata caaatttaac tggcatcagt
     9421 cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat aattcagact ggtcattcag
     9481 gcatccagac acgctaccgc ccctggcttt ttagctacca atacactgat ttagcttaat
     9541 ttttcaaacc ctctctgcat acaatcgttg atgagaaagg gttattgcgg aaattaactt
     9601 ccgaatataa ggtgacatta tggtaattga atattggctc tccaataatg caggaggaag
     9661 tgttacagct aacggaatag caggcaaggt aacaattcgg caattggcta tttttaagaa
     9721 ttatattaat tgtcattcaa tatggttttt aggagtttct ttaggttgac aatatttaat
     9781 attgcgcctc cacatgcgat atttcttaaa taatgtttta ttattacccc ttttaattca
     9841 gggataatgt gaggttatta ccctcaaata atatgagata aatagtgctg caacattgca
     9901 tttttgcccc gatttatcca tgatcgaatt gtgacatttg tcatacaacg aataggtttt
     9961 gtacttacta tggaatggat tgccgatccg tctatctggg ccgggttaat cacgctgatt
    10021 gtgatcgaac tggtcctcgg cattgataac ctggtcttta ttgccatcct cgccgaaaaa
    10081 ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg
    10141 cgcctgttac tgctggcgtc aatctcctgg ctggtcaccc tgactcaacc gctgttcagc
    10201 ttccgctcgt ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg
    10261 ttgttcaaag ccacgatgga gctgaacgaa cggctggaag ggaaagacag caataatccc
    10321 acacaacgca aaggtgcgaa gttctggggc gtggtgacgc aaattgtggt actggacgcc
    10381 attttctcac tcgactcagt gattaccgcc gtcgggatgg tcgaccattt actggtcatg
    10441 atggccgccg tggttatcgc tatcagcctg atgttgatgg ccagcaagcc gttaacccag
    10501 ttcgttaata gccacccgac catcgttatt ctctgcttaa gcttcctgtt gatgattggc
    10561 tttagcctgg tggcagaagg tttcggcttc gtcattccga aaggctacct gtacgctgcc
    10621 attggtttct cggtgatgat cgaggcactc aatcagttgg ctatctttaa ccggcgacgt
    10681 tttctttccg ctaatcagac gctacgccag cggactaccg aagcggtaat gcgcctgctt
    10741 agcgggcaaa aagaagatgc ggaactggac gccgaaaccg cgtccatgct ggtggatcat
    10801 ggaaaccagc agatctttaa tccgcaggaa cggcggatga ttgagcgggt acttaatctt
    10861 aaccagcgta ccgtcagcag cattatgacg tcgcgccacg atattgagca tatcgatctc
    10921 aacgcgccgg aagtagaaat ccgccagttg ctggagcgaa atcagcatac gcggctggtg
    10981 gttaccgacg gtgatgacgc agaagatttg ctcggtgttg ttcacgttat cgacctgcta
    11041 caacagtctc tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca gccgctggtg
    11101 ttcccggaaa ccttgccgtt gttacctgcc ctggagcagt tccgtaatgc ccgcacgcac
    11161 tttgcttttg tggtggatga gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc
    11221 actgaaacca ttgccggtaa cttaccgaac gaagtggaag agatcgacgc ccgccatgat
    11281 attcagaaga atgcggacgg ttcctggacg gcgaatggtc atatgccgct ggaggatctg
    11341 gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat atcacaccat tgccgggctg
    11401 ttgatggaat atttacagcg tattccaaaa cctggcgaag aagttcaggt gggggattat
    11461 ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga aggtgcagat tataccgctg
    11521 cgtaaggatg gcgagatgga gtacgaggtg tgatggtatc ggtcatttta cccgatagcc
    11581 gaaaggaata agatgaggga accgcgccag cggcgattta ccgccgggag cccgggttgc
    11641 cagggtgata gcgattgagc caccctggca cgttcaccgg cttgagcgtt ccatagtagc
    11701 gaggaacatc aagtgaacgg aacagccttc gatcttacat attcagaata taatgatgcc
    11761 gccacgaacg cctgatgcga ctctttacgc gtcttatcag gtctacagga caggtcatct
    11821 ggtaacctac atcttctcca gcaacttctt cacatccttg ccattgcggg aatctttatt
    11881 ccgctcggcc cagtcattca gacgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa
    11941 ctgatccact tgcaggctgt aattgagttg ctgccagttg ccataaaccc gcagcggtac
    12001 tggcgtttct ttcaggaaat caatcagttt actttccccg ttccagccac ccacgacccg
    12061 aatatcaaac tgggtgtcgc aggtttgatc tgccagattc aacatgcctt cccctgtcag
    12121 cgccagcaca ggcgattgac cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt
    12181 caaatcggtg gtaaagcggt caagccgcgt cacgttatca aagttttcag cggccttcac
    12241 atcaccgcca ttacgctcta ccgcttgctg aatcatctgc tggaagttca tcccttccat
    12301 gcgcgtgtcg gtcattttga catgtgcctg tccttgccag ttgtggcgga atgcgtcggc
    12361 atctatgtca gcaccggaga aatcaccagc cagtgacatt tttccggtca acgaaatcgg
    12421 atagttaaac gccttcagaa tggtaccaat ctcaacgttt tccagccgtg gctggaagtt
    12481 tatccgcgga tttattgatg tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt
    12541 aagcttgccc tgcagttgag taatttccag caaacccgac ttgttggtca tttgcgtggc
    12601 aacatctgta aaattcattc cgcgccagcg cacgttactg gcctgcaaca aaatatcagc
    12661 cgtaaagcct tgcagtccct gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg
    12721 cggcaatgtt gattggctct gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc
    12781 attaagcggg atgaggttgt caagattcag ttgcgggaat tgcagtctca gctgccattc
    12841 aggtttctct gtcagcgtga cctgtgcttg cccgctcagc gtgctgtcat tggcggtcaa
    12901 gctgatttgg ttaaatgaaa ggcgtttatg tgactcctgc cactgggcct ggaaactccc
    12961 ctgcccctga ataccttgtt ttggtaaatc tgcaccctgc aactgccagt taatttgttc
    13021 aatagccgcc gttaaatcat gcggataatc agaagcatct accgtaccgt taagggatat
    13081 cgtgagatcg cgctgatcgc gattaacccg cccggagaac tcaaatgagc cacgatgttg
    13141 gggatcttgt tccatttgca ggcggatatt gcggattgtc acctgctcgt catcttcatg
    13201 ctggaacacc agcacgctgt ccgccacctt aagactggat atatcaaacg accatccgcg
    13261 atcgtctgac agatccggca aggtattgtc gcgcggtgca accggagcgt cttcactgcg
    13321 caccgcttcc gtctgcggcg tcagttggat cactgcccct tttagcatca cctgcttaac
    13381 gctcagttga tgactcagta gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg
    13441 aaccagtggc tggcttgcgc cctgggcggt gagagacatt cgcccggaga ggatactaag
    13501 ctgcggccag acgtgccaac gcagtggccc gtcgagctgc aattgataac cgctacgtgc
    13561 agcaacttgc ttgaccatat agtcgcggaa atcattcgga ttcaccagca acactaacgc
    13621 agataacccg gccaccagca cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat
    13681 ggtatcctca atgggcctga attagtcttt atcgattcgg ctggctaccg cgccctgctg
    13741 gttgcgatat ttcgcatctt cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg
    13801 ctcaaagctc agcgcaccaa ttaacatgcc cggacgcagc gccagcggca gcttaccgga
    13861 gttgtagaac tccagcacaa tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac
    13921 gtgcaccatc agccccagac gcgccaaaga ggaacgcccg tccagccagc ccaccagatc
    13981 ggctggcagc gtcaccgact ccagcgtcac cgccagcgcc agctctcctg ggtgaagata
    14041 gaacgcctcg ccctcgtcga gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct
    14101 cacttcatct ttgggaccgc tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg
    14161 aaatttattg cccaggcgta catccaccgt cgcgccgtta atacgctcca ctggcggacg
    14221 tgggttgatc gacaaacggc cttcatcaag ccaggcttca atatctcggt cacacagacg
    14281 catggcattt tctcctttcg cgcatcactc ccttaacgcc aattacgtca agggcatacc
    14341 aggttatcac tgaacggtac acaatttatc aagcttattc aaagaactga cttattttcg
    14401 ctttcaatat atcgatcgcg atgcggtttt tcccgccgcg cggcacgata atgtccgcat
    14461 attgtttaga aggctcaatg aattgcagga acatcgggcg cacggttttt tgatattgcg
    14521 ccatcacgga atccattgaa cgcccacgct cgttaacgtc acgcttgatg cggcgcatca
    14581 ggcagatatc cagcggggta tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg
    14641 catccgtcag caacaaaatg ccttcgagaa tgatgacctt cttcggctca accgtcaccg
    14701 tttctttcat acgcgtatgt tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc
    14761 gtttcaacgc ttgtaaatgc tcaagcaaca ggctgtgatc catcgcgctg ggatggtcgt
    14821 agttggtctt aacgcgttct tccatcgaca gatggctttg atctttgtaa tagcagtctt
    14881 cgggaattac gccgatgtgt tcatcaccga cttgctcacg taattcacga taaagggtac
    14941 tggcaataag actcttgccg gaagccgatg cgccagcgat accgataatg acgcactgat
    15001 gagactgatc agtcatatat ttagcgacct gattaacctg gatgttagga agggggcgac
    15061 gaagcgccaa acgcggcaat tatagggatt tcatccgcct gataccagtc gaatagcgct
    15121 gccgcgcgct cagagttaat tgttgacgaa gaattcccgg tggcaaatta cgttgatcag
    15181 ttttatacaa ggtaaaaaat gttatacgca gttgcgcaaa ttatccgcct ttacgtcact
    15241 ttatgagcaa ttcgcatata aaatgtaaaa cttttgtact agcataaaca cagaaacgaa
    15301 tactggcgac ctggtcttgc ggataaagcg gtaatgagca aacaatcaca gcatgtatta
    15361 attgccctgc cccacccgct gcttcacctg gtcagtttag gtttagtctc gtttatcttt
    15421 acccttttct cgcttgagct ttcgcagttt ggcacccaac tcgccccact gtggttcccg
    15481 acgtccatca tgatggtggc gttttatcgc catgccgggc gcatgtggcc gggaattgcg
    15541 ctgagctgct cgctgggaaa tatcgccgca tccatcctgc ttttttccac cagctcgctg
    15601 aacatgacct ggacgaccat caatattgtt gaagccgtgg tcggggcagt gctgctacgt
    15661 aaattgctgc cgtggtataa ccccttgcaa aatctggctg actggctgcg tctggcattc
    15721 ggcagcgcca ttgttccgcc tctgctaggg ggtgttctgg ttgtcctgct gacgcccgga
    15781 gacgatcctc tcagggcatt tttgatatgg gtactgtcag aatccatcgg cgctctggca
    15841 ctggtgccgc tgggattgtt atttaaacca cactatctgc tgcgccatcg caacccacgg
    15901 ttgctttttg agtcgctgct cacgttagcc atcacactga cgttaagctg gctttcgatg
    15961 ctgtatctgc cgtggccttt tactttcatt attgtgctgt tgatgtggag cgccgtgcgc
    16021 ctgccacgaa tggaagcctt tttgatcttc cttaccacgg tgatgatggt gtcgctgatg
    16081 atggccgcgg atccctccct gcttgctacg ccgcgtacat acctgatgag ccatatgccg
    16141 tggctaccgt ttttgctgat cctgctgccc gccaacatca tgacgatggt gatgtatgcc
    16201 tttcgtgcgg aacgcaaaca catttccgaa agcgaaaccc gttttcggaa cgccatggaa
    16261 tattccgcta tcggtatggc gttagtgggc accgagggac aatggctgca aaccaacaaa
    16321 gcgctctgcc agtttctcgg gtacagtcag gaagagctgc gcggactcac ctttcagcaa
    16381 ctgacctggc cggaggatct caataaagat ctccaacagg ttgaaaagct gataagcggt
    16441 gaaataaaca cctattcaat ggaaaaacgc tactacaacc gcaatggcga tgttgtctgg
    16501 gcgttgcttg ccgtctcact ggtgcgccac acggatggca cgccgctcta ttttatcgct
    16561 cagattgaag acattaacga gctaaaacgc accgaacagg tgaatcagca actgatggag
    16621 cgcatcacgc tggctaacga agcgggcggg attggcatct gggagtggga gttgaagccg
    16681 aatattttta gctgggataa gcggatgttc gagctgtatg aaattcctcc acatatcaaa
    16741 ccgaactggc aggtgtggta cgagtgcgtg ctgccggaag atcgccagca tgccgaaaaa
    16801 gtgattcgtg attcgttgca atcacgctcg ccctttaaac tggaatttcg cattaccgta
    16861 aaagacggta ttcgccatat ccgcgccctc gccaaccggg tactgaataa agaaggcgaa
    16921 gtcgaacgcc tgctcggcat taatatggat atgactgagg ttaaacagct taacgaggca
    16981 ttgtttcagg aaaaagagcg cctgcacata acgctggact ccatcggcga agccgtggtc
    17041 tgtattgata tggcaatgaa aattaccttt atgaatccag tggcggagaa gatgagcggc
    17101 tggacgcagg aagaagcatt aggtgttccg ctcctgacgg tgttgcatat tacctttggc
    17161 gacaatggtc ccctgatgga gaacatttac agtgccgaca cctcacgttc cgcgattgaa
    17221 caagatgtgg tgttgcactg ccggagcggc ggcagctacg acgtgcatta cagtattacg
    17281 ccgttaagta ctctggacgg cagcaatatt ggttcggttc tggtgattca ggacgttacc
    17341 gaatcacgca aaatgctgcg ccagctgagc tacagcgcct cccatgatgc actgacgcat
    17401 ctcgccaatc gcgccagttt tgagaagcaa ctgcgtatgc tgctgcaaac ggtaaacagt
    17461 acacatcagc gacatgccct ggtgtttatc gatcttgatc gctttaaagc ggtgaatgac
    17521 agcgccgggc atgcggcggg cgacgctttg ctgcgcgaac tggcgtcgtt gatgctgagt
    17581 atgctgcgtt ccagcgacgt gctggcgcga ctcggtgggg atgaatttgg tctgctgcta
    17641 ccagactgta atgttgaaag cgcgcgtttt atcgctacac gcattatcag tgccgtgaat
    17701 gactatcact ttatatggga aggccgtgtg catcgggtag gtgccagtgc cgggattacc
    17761 ttgattgatg acaacaatca tcaggcggca gaagtgatgt cgcaggctga tatcgcctgt
    17821 tatgcctcca aaaatggtgg acggggccgg gtgacggttt acgaaccaca gcaagctgcc
    17881 gcacatagcg aacgggcggc gatgtcgctt gatgaacagt ggcggatgat taaagagaat
    17941 cagttgatga tgatggccca cggtgtcgct tcgccgcgga tcccggaagc gcgtaatttg
    18001 tggctgattt cgcttaaact ctggagttgc gaaggcgaga ttattgatga acaaaaattt
    18061 cgtcgtagct tcagcgatcc ggcacttagc catgctcttg accgacgggt attccacgaa
    18121 tttttccagc tggccgcaaa agcggttgcc agtaaaggca taagcatcgc cctccccctt
    18181 tccgttgccg gtttgagtag cgccacgctg gtgaatgatc tgcttgagca gctggaaaat
    18241 agccctttac caccacggtt attacacctg attattccgg ctgaagcgat tttagatcac
    18301 gcagaaagcg tgcaaaaact gcggctggcg ggatgtcgga tagtgctcag ccaggtgggc
    18361 cgcgatctgc aaatcttcaa ctcgctgaaa gcgaatatgg cagattacct gctacttgat
    18421 ggtgagttat gcgccaacgt gcagggtaat ttgatggatg agatgctgat tacgattatt
    18481 caggggcacg ctcagcgact cgggatgaaa accatcgccg ggccagtcgt tttaccctta
    18541 gtgatggata cgctttctgg catcggcgtc gatctgattt atggtgatgt gattgccgat
    18601 gcccaaccgc tggatttgct ggtgaatagc agttatttcg cgattaactg agggagcgtt
    18661 tgcccattgc ctgatgcgac gtatcaggcc tacaattcgt ccgttccgtc tggttgccag
    18721 ccttccgtat accagatatg caacagcgca taagaacgcc agggcttcca gcgctcggca
    18781 taacggcgga tttgtgccgg tgtcatcccc ggaaatcgct gtttaatcag ataatcatcc
    18841 ggcagaaaaa catctttcgc ctgccagcca cgcaaagcga aataattcgc cgtccagcgc
    18901 ccgatacccg gaaaagtttg cagcgttttc atcgcctgct ccacatcgcc cggaattgtc
    18961 attggtaagc tgccctccag cgccgcattt gccagatgaa tcagtgcctc tgcccgtttc
    19021 aacggcatgc ctaacgcttt taatgcctgc gggtcggctg ctgccagccg ctgaggcgtg
    19081 gggaagcaga tatactccgg aaaatcatcc agccgttcgc cataaagctg tacaactttg
    19141 gcagtcaatt ttgccgccat cgccacgctc accagttggc ctaaaatcgc ccgcacaccc
    19201 tgctcaaaag catcaataca gccgggtaaa cgcaatccgg gccgcgccgc gcctaacttg
    19261 cccaacgcac cgttaacaat ctgcgggtta cattgcagat caaacaggcg gctcattttc
    19321 gccagacact cagcggcaac gggttctaaa cctgcactta aatttatgtg cagagtatgg
    19381 cgggctatat ccggaatagc agtcaccacg ccgcgatatt cgcccaccgc cagactacgg
    19441 gcataataat cctcagcgac cgtttccaca ccactcaccg cacgggcggc gagaaatccc
    19501 aacatccacg accagtcata cggcggctgc cagttcaggg tatacatcgc atctccttat
    19561 tcatccgctt tcagcataaa cgttattcag acgctacgct ttgctttcat attccggttg
    19621 tcgcgacggc aacatttcgc taaagtcacg ccccttcttc accggcatgg ggattaattc
    19681 gtgtttattg gttttgatta cggtacagca aactgttcag tggcggtcat gcgtgacggt
    19741 aaaccgcaat tactgaaaat ggaaaacgac agcacgctgc tgccttcaat gctttgcgcg
    19801 ccaacgcgtg aagcagtaag tgaatggctg taccgccatc atgatgttcc ggcagacgac
    19861 gatgaaacgc aggcgctgtt acgtcgggcg attcgttata accgcgaaga agatatcgat
    19921 gttacggcga aaagcgtgca gttcggtctt tcctcactgg cacagtacat tgatgatcca
    19981 gaagaagtgt ggtttgtgaa atcaccaaaa tcgttcctcg gtgccagcgg cttaaaaccg
    20041 cagcaggtag cgctgtttga ggatctggtc tgcgcaatga tgttgcacat tcgccagcag
    20101 gcgcaggcgc agctgccaga agcgattact caggcggtga ttggtcgccc gatcaacttc
    20161 caggggctgg gcggcgatga agcaaacgcc caggcgcaag ggattctgga acgcgcggcg
    20221 aagcgtgccg gattcaggga cgtggtattc cagtacgagc cggtcgcggc tgggctggat
    20281 tacgaagcca ccttgcagga agaaaaacgg gtgctggtgg tggatatcgg cggtggtacg
    20341 actgactgtt cattgctgct gatggggccg cagtggcgtt cgcgtctcga tcgtgaagcc
    20401 agcctgctgg gtcacagtgg ttgccgtatt ggcggtaacg atctggacat cgcactggcg
    20461 tttaaaaacc tgatgccgtt actgggcatg ggtggcgaaa ccgaaaaagg catcgccctg
    20521 ccgattctgc cgtggtggaa tgcggttgcc atcaacgacg tacctgcgca gagtgatttc
    20581 tacagtagtg ccaacggtcg tctgcttaac gatctggtac gcgatgcccg cgaaccggaa
    20641 aaagtggccc tgctacagaa agtctggcgt cagcgtttaa gctatcgcct ggtgcgtagc
    20701 gcagaagagt gcaaaattgc tctttcaagc gtagcggaaa cccgcgcctc actgccgttt
    20761 atcagcgatg aactggctac gctgattagc cagcaagggc tggaaagcgc cctcagccag
    20821 ccactggcgc ggattctgga acaggtgcaa ctggccctgg ataacgccca ggaaaaaccg
    20881 gacgttatct acctgaccgg cggtagcgcc cgttcgccgc tgattaaaaa agcgctggca
    20941 gaacagttgc cgggaattcc gattgcaggc ggcgatgact ttggctccgt caccgccggg
    21001 ctggcacgct gggcggaagt ggtgtttcgt taaaagaaaa tggctcagca gcggcttaaa
    21061 tctgcctgcg cctgagccag tttttctgcc gcctgacgta aaggttccat caacggcatg
    21121 gtccgtgctg gagcatgaag gcggaatcgc tgcaactctg tcgcgccgac agtcaacgtc
    21181 gattccagcc gatgtcgttt ggcggtcatt ttcgccatta ccgcttgtct gtctgctggc
    21241 gttatcgctt cattcggcct gaacacaaaa cggcgttcac agctcgcttt ccagtcgata
    21301 accgcctggg tcagatggtc gccaaaccct ttcacttgct taacgccacg acgggtaaca
    21361 tctgcggctg tttcaatacc aaaagaccgt aacgccgctt tacgcgcagg gccaacgccg
    21421 ggaatagagg caacatcaat aaaaaatccc tccagaaact tctgcttctg ccgttccctt
    21481 gcagtgtcat gaagtgctgc cagagcgcgt ttctcttctt ccggtaaccc gagaatttcg
    21541 tccttcattt tttcgagcat cgcccgtttg gcgataaaac cttccagccc gcccaactgt
    21601 tggctctggc tgactaaatg gtcatagtcc attttcgcgc gattaaatgc ctcacggcgt
    21661 tgctggattt ctgctttata agctttgctt gtcagactgc caataatcca gatagccgcc
    21721 agcacaggaa ccaaaataat ataacgcggt tctgcctgaa ggccgcaaag cagcagcgat
    21781 aatgctgaca gtgcgatctc aagcagaatg atgtattcgc gccgtaacag gcctaaagga
    21841 agcggcctgc cagtcggttg gaaatgatcg ggtaatggca gttgcaatgc tggcggtgct
    21901 actgacgcca tcaccatcgc ccagactttc gccagcacaa aatcaccgcc ggtggtaatg
    21961 acctcttcgc cgagatcaat aaaatagata acgccttgat tatccagcgt acaccacggg
    22021 cagtcggtca aatgagcggg ataaacatgc attgccgaaa cgggacattt ctttaactgt
    22081 tggcgcagag aatcaagtgc cgctacccac gctttagccg tcggacgccc ggttgccacg
    22141 ccactttccg tgaatgcctg ctgaaacatg gcttcaacat cactcggtaa catcgatagc
    22201 ggaatcgatc gtggcggcgg ttttaaacca cgtcgctgat tatctgacgc gtaggcataa
    22261 cggaaatggg taatatccgt ctccagcgca ttacccgcat cagagataag cggcacacca
    22321 gaataaggat gccggccacc aaacaagacg tgaaaaatca gcaacgcaag gccaaaatta
    22381 tcgtgatttt ttgtgcgttc aaagccgaca aatgacgaca aggtttgtag ctctggcggc
    22441 gtaaaatgcg acacgccgac ttcgcataaa tgcagtgtgc cattggcgtt aatctgaaag
    22501 gagtcgctat caatcagtac cactttgctg tcgcgaccta ccataaaact gttctggttt
    22561 acgtcaccca cgacgtgccc gtgctcgtga accgtagcaa aagatgaagc aatattgcgc
    22621 gcaacataga gtagaaaatc ccacgcacaa tgagggtaac gttgacgacg atgtgccggg
    22681 ctatagatca tatgaatcgg ttctttacca gaaacttttg gcatcataaa accgataact
    22741 tttccgcctc gtccaccgtg aagcgttgcc tgcggccagg cgacataatt caacaattgc
    22801 gcgtcagctg tcgcagccat aaaggcaagt ttgtcctgtt ttaaggcggg tggcggcgtg
    22861 tgataaatct tggcgacgct atcgacaaac tcattgatat cataaaccgc gccttcaccg
    22921 cctttgccca gttcaagacc gagagtggtc atttcacctg ttgatgtaaa tacttttata
    22981 tttgtcttca taatgtttca agggcagcaa tattatattt caatgaataa taacactgcc
    23041 cattataatt atttctatat gggaagagcc cactcaagaa taaaaatgat aatgtaattt
    23101 catcaggata caattcaatt ccaacctgat aatgattcac ttccacaggt tttgctaact
    23161 tttactacac agagattctg gagaaaaaat gaaaaagata ttactcatgt tgagcctgct
    23221 attttttact accgcgggct ttagtgaagt ttcggatacg ttagtgacag gaggttatga
    23281 taaacaagcg atgtctgacg cgattaaaca tgcgcggaaa gaaaccgata aattcatcga
    23341 ggttatgaat aaaaaagatg ccgatacttt cgcagtcaaa gccccgataa ccgatcacgg
    23401 aagaaccgag catttctggc tcacggatgt cacttattct aacggcatgt ttattggcgt
    23461 aatcagcaat gaccctggta ttgttacaaa cgtagaatat ggccaggagt ggaaaatcaa
    23521 gaaagaagat atttcagact ggatgtatac gcgcggggat aaaatttacg gcggttatac
    23581 cattgatcct ttattggtca cctatccaaa agaagaagcc gacgagctta gagcaaaatt
    23641 agttcgctaa tcagccgtta tatttattgt ttaaactggc ggttgaattg ttgtcttcaa
    23701 ccgccatgag aaactcactc tgcccacagc gccagtgcta acgttttatc atcatcggta
    23761 cgctcgttta ccgctgggct ggacaaaaac tgctttaaca attcaggtaa taaatcgagt
    23821 tgttcctgcg ttgctgacgc cagtccattg aaaaacgggg taaagaaagg cacatgcgga
    23881 gaattatcca acatatttaa cgccagtcgc tggataccat ccgtaaatgc tgcaacttta
    23941 tgcacacgct cagtgctggt gaaagtttcc agtctggaaa cggcatcttc atcggtaatg
    24001 aagtgcgtca tattggcata ttcacccacc atcggcgtaa gcggcaattg cagaccgtga
    24061 ccaaaatcca ccaccacgcc gccatcgcca atctgcataa tcaaggtgcc attagccgat
    24121 gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaaccg ctaattcttt ggcctctgcc
    24181 tcggcaaaaa gtcgctgacg aatagtcagc acaatatccg tggcgaggat atcattaagc
    24241 cccaattccc caccctgcac tttttgcgac atataagcca tcgcttcatt gacggcgagc
    24301 atcgcgcctt cgccaccctg cgagacgctg cctgcaccat cagcaagaaa catcaccagt
    24361 aatggttgct gatcgtttaa ccaggcaacc tgcatctgac aggcatcctg acaaggtaaa
    24421 tcagcgctaa tgtgcgacgt tccaacagcg gatgcataaa ccagacgcca gctcacactg
    24481 acgtccagcc ttttggcgct tccagcacaa cttccgttcc cggcgtggag cgggaaaccg
    24541 aacgcagcga actggataac cagctaaaca gttcacggaa ttgtaatcct tgcagaggca
    24601 aaggctgacg aacgctgatt tgtgccagcg tcttcatatc cgccccctga acagcgatgg
    24661 aaaagaaggc aaacttctta tcttcttccc cctgaaacac tttgttggcg gcagcctgcc
    24721 actcatcggt tggagcgcca tcggtgatca ggaaaatcca cgggcgataa tacgaaatac
    24781 catttgcgcg gtactcacgt ttcctctctt caaccatatc cagggctttg gtaatggcgg
    24841 cccccattgg cgtatccccc tgggcaaaca ggatgggcgg gaaaaagtta gcggcgctgg
    24901 taaagggttg ttccacatgc accgggccga acgtcacaat accgagttcc actcttttta
    24961 aggccagcga atcggcaagc agctcatcgc gaaaggtaac caatccggcg ttaagttcat
    25021 taatgggtct gccgctcatc gagccagaaa catccagcag caaaatgcag gggcaacgtg
    25081 gttccgggtt actggcaaaa tcgctggtgg caaacgtgat ttgttcgctc ataagaaatc
    25141 ctttcttaga gaattattga ggtccggtat cataccgact gcaataatga aataagagca
    25201 aacgaagaaa ctttcagaat taggcccggc ggcgcagaaa attttgctat tcgcctcgca
    25261 agaacggaga gccgtcgatt gacactaagg gcggagtgac ataatttcag gagtgagggt
    25321 tagggagagg tttccccctc cccctggtgt tcttagtaag cctggaagct aatcactaag
    25381 agtatcacca gtatgatgac gtgcttcatc ataacccttt ccttattaaa agccctcttc
    25441 tccgggagag gctttcccgt ttcagcgtcc cgctgaaatc atcggcttac ctcctttcgc
    25501 cctgaatgca gtctatcgct aacgcgtgaa taaaatagtt tcctctgtga ttattgcgta
    25561 acgactcgca aagaatattt gctggcagga tcgcagacta caaagcctgc ggattgacaa
    25621 tcttatcgtg aaggcatact ttcaggagtg agggtagagc ggggtttccc ccgccctggt
    25681 agtcttagta agcggggaag cttatgacta agagcaccac gatgatgagt agcttcatca
    25741 tgaccctttc cttatttaag gccccttcct cgggaggggc tttcccgttt cagcgtcccg
    25801 ctgaaatcgt cggcttacct cctttcgcca tgcacttagt ctatcgctga cttgcagata
    25861 aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa gaatttttgc tggcaggatc
    25921 gcagacggca aagcctgcgg attgacaatc ttatcgtgaa ggcatacttt caggagtgag
    25981 ggtagagcgg ggtttccccc gccctggtag tcttagtatg ctgggtagct aatcactaag
    26041 agcaccacga tgatgagtag cttcatcatg accctttcct tatttaaggc cccttcctcg
    26101 ggaggggctt tcccgtttca gcatccggct gaaatctgcg gcttacctcc tttcgccctg
    26161 tatgcagtct atcgctaacg cgtagataaa atagtttcct gtgttattac tggatgcgtg
    26221 ctcgcaaatg tgcccgccat tcagacgatt cccgatagtg tttcataatt cctccatttt
    26281 tctcccttat tggctggcta cactagtatc attccgcgaa acgtttcagg aagagaaact
    26341 cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc gtaatcgtgg tggttatcgc
    26401 cgccatcgcc gcattctggt tctggcaagg ccgcaatgac tcccagagtg cagccccagg
    26461 ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc cgtggtatgc gttccggccc
    26521 attagccccg gttcaggcgg cgaccgccgt agaacaggca gttccgcgtt acctcaccgg
    26581 gcttggcacc attaccgccg ctaatactgt tacggtgcgc agccgcgtgg acggtcaact
    26641 gatggcgtta catttccagg aaggccagca ggtcaaagca ggcgatttac tggcagaaat
    26701 tgaccccagc cagttcaaag ttgcattagc acaagcccag ggccaactgg caaaagataa
    26761 agccacgctt gctaacgccc gccgtgatct ggcgcgttat cagcaactgg caaaaaccaa
    26821 tctcgtatcc cgtcaggagc tggatgccca acaggcactg gtcagtgaaa ccgaaggcac
    26881 cattaaggct gatgaagcaa gcgtcgccag cgcgcagctg caactcgact ggagccgcat
    26941 caccgcacca gtcgatggtc gcgttggtct caagcaggtt gatgttggta accaaatctc
    27001 cagtggtgat accaccggaa ttgtggtgat cacccagacg catcctatcg atttagtctt
    27061 taccctgccg gaaagcgata tcgctaccgt cgtgcaggcg caaaaagccg ggaaaccgct
    27121 ggtggtagaa gcctgggatc gcaccaattc aaagaaatta agcgaaggca cgctgttaag
    27181 tctcgataac caaatcgatg ccactaccgg tacgattaaa gtgaaagcac gctttaataa
    27241 tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg cgcatgttag tcgacaccga
    27301 acaaaacgcc gtagtgatcc caacagccgc cctgcaaatg ggcaatgaag gccattttgt
    27361 ctgggtgctg aatagcgaaa acaaggtcag caaacatctg gtgacgccgg gcattcagga
    27421 cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc gatcgcgtgg tgacagacgg
    27481 cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg gaagcccaga gcaccaccac
    27541 ttcggaagag aaagccacca gccgcgaata cgcgaaaaaa ggagcacgct cctgatgcag
    27601 gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt ttattatgcg tcctgtcgcc
    27661 accacgctgc tgatggtggc gatcttactc gccggaatta tcggttatcg cgccctgccc
    27721 gtttcggcac tgccggaagt ggattatccg actattcagg tggttacact ctaccctggc
    27781 gccagcccgg atgtgatgac ctctgccgtt accgcgccgc tcgaacgcca gttcgggcag
    27841 atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg gtgcgtcagt tatcactttg
    27901 cagttccagc taacattacc gctcgatgtc gccgagcagg aagtgcaggc cgcgattaac
    27961 gctgcgacca acttgttgcc gagcgatctg cctaacccgc cggtttacag caaagtgaac
    28021 ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa ccgccatgcc gatgacccag
    28081 gtagaagata tggtggaaac ccgcgtcgcg cagaaaatct cgcagatttc cggcgtcggc
    28141 ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg tcaaacttaa cgctcaggcg
    28201 attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg ccattaccgg cgctaacgtt
    28261 aactcggcaa aaggtagcct cgacggccct tcccgtgcgg tcacgctttc cgcgaacgac
    28321 cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg cctaccagaa cggcgcgcca
    28381 attcgtctgg gcgatgtcgc aactgtagag caaggtgcag aaaacagctg gctcggcgcg
    28441 tgggcgaaca aagaacaggc cattgtgatg aatgttcagc gccagcccgg tgctaacatt
    28501 atctccaccg ccgacagcat tcggcagatg ctgccacagc tcactgagag tctgccgaaa
    28561 tcggtgaagg tgacagtgct ttccgatcgc accaccaata tccgcgcatc cgtcgatgat
    28621 actcagtttg aattgatgat ggctatcgcg ctggtagtca tgattatcta cctgtttttg
    28681 cgcaatattc cggcgaccat cattcccggt gttgctgtac cgctgtcgtt aatcggcact
    28741 ttcgcggtta tggtgtttct cgatttttca atcaataacc tgacactgat ggcgttaact
    28801 atcgccaccg gattcgtggt cgatgacgcc atcgtggtga tcgaaaacat ttcccgctat
    28861 atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaagg gcgcaggtga aatcggcttt
    28921 accattatct cgctgacctt ctcactgatt gcggtgttga tcccactgct gtttatgggc
    28981 gatatcgtcg ggcgactgtt ccgcgaattt gctattacgc tggcggtagc gattttgatc
    29041 tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg cgcggatgct cagccaggag
    29101 tcgttgcgta aacagaaccg cttctcccgt gcctcggaaa aaatgttcga caggataatc
    29161 gccgcctatg gtcgtggact ggcgaaagtg ctgaatcatc cgtggctgac cttaagcgtg
    29221 gcactcagca cgctgctgct tagcgtgctg ctgtgggtgt tcattccgaa aggtttcttc
    29281 ccggtacagg acaatggcat tattcagggc actttgcagg caccgcaatc cagctccttt
    29341 gccaatatgg cccagcgaca acgccaggtc gcggacgtga ttttgcagga tccggcagtg
    29401 caaagcctga cctcatttgt tggcgttgat ggcactaacc cgtcgctgaa cagtgcacgt
    29461 ttacaaatca acctcaaacc gttggatgaa cgtgatgatc gggtgcaaaa agtcatcgcc
    29521 cgtctgcaaa cggcggtaga taaagtgccg ggcgtcgatc tcttcctgca accaacgcag
    29581 gatctgacta ttgatactca ggtcagccgc acccagtacc agtttacctt gcaggccacc
    29641 tcactggatg cgctcagtac ctgggtgcca cagttgatgg aaaaactcca gcaactgcca
    29701 cagctttctg atgtctccag cgactggcag gacaaagggc tggtggcgta tgtcaatgtt
    29761 gatcgcgaca gcgccagccg tctggggatc agcatggcgg atgtcgataa cgccctgtac
    29821 aacgcgtttg gtcagcggct gatttccact atttatactc aggccaacca gtatcgcgtg
    29881 gtgctggagc acaacaccga aaatacccca ggcctcgcgg cgctggatac cattcgcctg
    29941 accagcagcg acggcggcgt ggtgccgcta agctcaattg ccaaaattga gcagcgtttt
    30001 gcgccgctct ccatcaacca tctggatcag ttcccggtaa cgaccatctc ctttaacgtg
    30061 ccggataact attcgctggg cgatgcggtg caggcgatta tggacaccga aaagacgctg
    30121 aatctgccgg tggatatcac cacgcagttc cagggcagca ccctcgcttt ccagtcggcg
    30181 ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg cgatgtatat cgtgctcggc
    30241 attctgtacg agagctttat tcacccgatc accattctct cgacgctacc caccgcaggg
    30301 gttggcgcac tgctggcgtt gctgattgct ggtagcgaac tggatgtgat tgcgattatc
    30361 ggcattattt tgctgatcgg tatcgtgaag aagaacgcca tcatgatgat cgacttcgcg
    30421 ctggctgccg agcgcgagca aggcatgtcg ccgcgcgagg caatctacca ggcttgtctg
    30481 ttgcgttttc gtccgatcct gatgaccact ctggcggctc tgcttggcgc gctgccgctg
    30541 atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt taggtatcgg catggtcggc
    30601 ggtctgattg tcagccaggt gctgacgctg tttaccacgc cggtgattta tttgctgttc
    30661 gaccgcctgg cattgtggac caaaagccgc tttgcccgtc atgaagagga ggcgtaagtg
    30721 aagttttttg ccctcttcat ttaccgcccg gtggcgacga ttttactgtc ggttgccatt
    30781 accctgtgcg gcatactggg cttccgtatg ctgccggtcg ccccgctgcc gcaggtcgat
    30841 tttccggtga ttatggtcag cgcctcgctg cccggtgcgt caccagaaac aatggcgtct
    30901 tccgttgcca cgccgctgga gcgctcactt gggcgcattg ccggagtcag tgaaatgacc
    30961 tccagcagtt cgctcggcag cacgcgtatt attttgcagt ttgattttga ccgggatatc
    31021 aacggcgcgg cgcgcgatgt gcaggcggcg atcaacgctg cacaaagttt gctgcccagc
    31081 gggatgccca gccgtccgac ctatcgcaaa gctaacccgt cggatgcgcc aattatgatc
    31141 ctcacgctga catccgatac ttattcgcag ggtgaactgt acgattttgc ctcgacgcag
    31201 ctggctccga cgatttcgca aatcgacggt gttggtgatg tcgatgtcgg cggcagctca
    31261 ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt ttaatcaggg cgtctcgctg
    31321 gacgacgtac gcaccgccat cagcaatgcc aacgtgcgta aaccgcaggg ggcgctggaa
    31381 gatggcactc accgctggca gatccagacc aatgatgagc taaaaaccgc cgccgaatat
    31441 cagccgctga ttattcatta caacaacggc ggcgcggttc gtctgggcga tgtggcgaca
    31501 gtgaccgact ctatacagga tgtacgtaac gccgggatga ccaatgccaa accggctatt
    31561 ttgctgatga tccgcaagct gccggaagcc aatattatcc agacggttga cagcatccgg
    31621 gcaaaattac cggagttgca ggaaaccatt ccggcggcga ttgatctgca aattgctcag
    31681 gatcgctccc ccaccattcg cgcctcgctg gaagaagtcg agcaaacgct gattatcttg
    31741 gtggcgctgg tgattctggt ggtgttttta ttcctgcgct cgggtcgcgc aactattatt
    31801 cccgccgttg cggtgccggt ttcgctgatt ggtacgtttg cggcgatgta cctgtgcggt
    31861 ttcagtctca ataatctttc gttaatggcg ctcaccatcg ctactggttt cgtggtggat
    31921 gacgccatcg tggtgctgga aaacattgcc cgtcatctgg aagcgggaat gaaaccgttg
    31981 caagccgcac tgcaaggtac tcgcgaagtc ggttttacgg tgctgtcgat gagtctgtca
    32041 ctggtggcgg tgttcctgcc gctgctgttg atgggcggat tgccgggccg actgttacgc
    32101 gaatttgccg tgacgctttc tgtcgccatt ggtatatcgt tgctggtttc tctgacatta
    32161 acgccaatga tgtgtggctg gatgctgaaa gccagcaagc cgtgcgagca aaagcgactg
    32221 cgtggttttg gtcgcatgtt ggtagccctg caacaaggct acggcaagtc actgaaatgg
    32281 gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg gcaccattgc gctgaatatc
    32341 tggctgtata tctcgatccc gaaaaccttc ttcccggagc aggacactgg cgtgttgatg
    32401 ggcgggattc aggcggatca gagtatttcg tttcaggcga tgcgcggtaa gttgcaggat
    32461 ttcatgaaaa ttatccgtga cgatccggca gtggataatg tcaccggctt tacaggcggt
    32521 tcgcgagtga acagcgggat gatgtttatc accctcaagc cacgcgacga acgcagcgaa
    32581 acggcgcagc aaattatcga ccgtctgcgc gtaaaactgg cgaaagaacc gggggcgaat
    32641 ctgttcctga tggcggtaca ggatattcgc gttggtgggc gtcagtcgaa cgccagctac
    32701 cagtacacgt tgttatccga cgacctggcg gcactgcgag aatgggagcc gaaaatccgc
    32761 aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact ccgatcagca ggataacggc
    32821 gcggagatga atctggttta cgaccgcgac accatggcac ggctgggaat cgacgtacaa
    32881 gccgccaaca acctgttaaa taacgccttc ggtcagcggc aaatctcgac catttaccag
    32941 ccgatgaacc agtataaagt ggtgatggaa gtggatccgc gctataccca ggacatcagc
    33001 gcgctggaaa aaatgttcgt tatcaataac gaaggaaaag cgatcccgct gtcgtatttc
    33061 gctaaatggc aaccggcgaa tgccccacta tcggtgaatc atcagggatt atcggcggcc
    33121 tcgaccattt cgttcaacct gccgaccgga aaatcgctct cggacgccag tgcggcgatc
    33181 gatcgcgcaa tgacccagct tggtgtgcct tcgacggtgc gcggcagttt tgccggaaca
    33241 gcgcaggtgt tccaggagac gatgaactca caggtgatcc tgattatcgc cgccatcgcc
    33301 acggtgtata tcgtgctggg tatcctttac gagagttatg tgcacccgct gacgattctc
    33361 tccaccctgc cctcggcggg tgttggagcg ctgctggcgc tggagctgtt caatgccccg
    33421 ttcagcctaa tcgccctgat agggatcatg ctattaatcg gcatcgtgaa gaaaaacgcc
    33481 attatgatgg tcgattttgc gcttgaagcc caacggcacg gtaacctgac gccgcaggaa
    33541 gctattttcc aggcctgtct gctgcgtttt cgcccgatta tgatgactac cctggcggcg
    33601 ctgtttggcg cgctgccgct ggtgttgtcg ggcggcgacg gctcggagct gcggcaaccc
    33661 ctggggatca ccattgtcgg cggactggta atgagccagc tccttacgct gtataccacg
    33721 ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt tttcgcgtaa acctaaacaa
    33781 acggtaaccg agtaaatgac agatcttccc gacagcaccc gttggcaatt gtggattgtg
    33841 gctttcggct tctttatgca gtcgctggac accaccatcg taaacaccgc ccttccctca
    33901 atggcgcaaa gcctcgggga aagtccgttg catatgcaca tggtcattgt ctcttatgtg
    33961 ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg cggacaaagt cggcgtgcgc
    34021 aatattttct ttaccgccat cgtgctgttt actctcggtt cactgttttg cgcgctttcc
    34081 ggcacgctga acgaactgtt gctggcacgc gcgttacagg gcgttggcgg cgcgatgatg
    34141 gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc gcgagcaata tatggcggcg
    34201 atgacctttg tcacgttacc cggtcaggtc ggtccgctgc tcggtccggc gctcggcggt
    34261 ctgctggtgg agtacgcgtc gtggcactgg atctttttga tcaacattcc ggtggggatt
    34321 atcggtgcga tcgccacatt gctgttaatg ccgaactaca ccatgcagac gcggcgcttt
    34381 gatctctccg gatttttatt gctggcggtt ggcatggcgg tattaaccct ggcgctggac
    34441 ggcagtaaag gtacaggttt atcgccgctg acgattgcag gcctggtcgc agttggcgtg
    34501 gtggcactgg tgctttatct gctgcacgcc agaaataacc accgcgctct tttcagtctg
    34561 aaactgttcc gcactcgtac cttttcgctg ggcctggcgg ggagctttgc cggacgtatt
    34621 ggcagtggca tgttgccctt tatgacaccg gttttcctgc aaattggcct cggtttctcg
    34681 ccttttcatg ccggactgat gatgatcccg atggtgctcg gcagcatggg gatgaagcga
    34741 attgtggtac aggtggtgaa tcgctttggt tatcgtcggg tactggtagc gaccacgctg
    34801 ggtctgtcgc tggtcaccct gttgtttatg actaccgccc tgctgggctg gtactacgtt
    34861 ttgccgttcg tcctgttttt acaagggatg gtcaactcga cgcgtttctc ctccatgaac
    34921 accctgacgc tgaaagatct cccggacaat ctggcgagca gcggcaacag cctgctgtcg
    34981 atgattatgc aattgtcgat gagtatcggc gtcactatcg ccgggctgtt gctgggactt
    35041 tttggttcac agcatgtcag cgtcgacagc ggcaccacac aaaccgtctt tatgtacacc
    35101 tggcttagca tggcgttgat catcgccctt ccggcgttca tctttgccag agtgccgaac
    35161 gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga gcgcgcaatg aagttctggc
    35221 gacccggtat taccggcaaa ctgtttctgg cgattttcgc cacctgcatt gtcttgctga
    35281 tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg ctttattgat tacatcaagc
    35341 atggtaatga acagcgatta caactgttaa gtgatgcgct tggcgagcag tatgcgcagc
    35401 atggcaactg gcgcttcctg cgcaacaatg atcgctttgt ctttcagatc ctgcgttcat
    35461 ttgaacacga taattcggaa gataaacccg gcccgggtat gccaccgcac ggctggcgta
    35521 cccagttctg ggtggttgat caaaacaaca aagtgctggt tggtccgcga gcgccgattc
    35581 cacctgacgg tacacggcga cccattctgg tcaacggtgc ggaagttggc gcggtgatcg
    35641 cctcccccgt tgagcggtta acgcgcaata ctgatatcaa tttcgataaa caacagcggc
    35701 aaaccagctg gttgattgtc gccctggcaa cgttactcgc ggcacttgcc acttttctgc
    35761 tggcgcgcgg tttactggca ccggtaaaac gacttgtcga tggcacgcac aaactggcgg
    35821 cgggcgattt cactacccgc gtaacgccca ccagtgaaga tgaactgggc aagctggcgc
    35881 aagacttcaa ccagcttgcc agcacgctgg agaaaaacca gcaaatgcgg cgcgatttta
    35941 tggccgatat ttctcacgaa ctgcgtacgc cattagcggt gctgcgcggt gaactggaag
    36001 ccattcagga tggcgtgcgt aaattcacgc cggagacggt ggcgtcttta caggcggagg
    36061 tcggtacact gaccaaactg gttgacgatc tccatcagtt gtcgatgtct gatgaaggcg
    36121 ctctcgccta tcaaaaagca ccggtagatt tgatcccact gctggaagtg gcgggcggcg
    36181 catttcgcga acgatttgcc agccgtggcc tgaaactgca attttccctg ccagacagta
    36241 ttaccgtatt tggcgatcgc gaccgtttaa tgcagttatt caataactta cttgaaaaca
    36301 gcctgcgcta cactgacagc ggcggcagcc tgaaaatctc tgccgagcag cacgacaaaa
    36361 cggtgcgcct gacctttgcc gacagcgcac cgggcgtcag tgacgatcag ctacaaaaat
    36421 tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg tgccagcggc ggttccgggc
    36481 tggggctggc gatttgcctg aacattgttg aagcacataa tggccgcatt attgctgccc
    36541 attcgccttt tggcggggta agcattacag tagagttacc gctggaacgg gatttacaga
    36601 gagaagtatg accgagttac caatcgacga aaacacaccg cgtattttga tcgtggaaga
    36661 tgaaccgaag ctggggcagt tgctcattga ttatctgcgt gctgcgagct atgcgccgac
    36721 gcttatcagc cacggcgatc aggtactggc gtatgtgcgc cagacaccac cggatctgat
    36781 cctgttggat ctgatgctcc ctggcaccga tggcttgacg ctgtgccggg aaattcgtcg
    36841 tttttctgac attcctatcg tgatggtgac ggcaaaaatc gaagagatcg accgcctgct
    36901 cggactggag attggcgcag atgactacat ctgcaagccg tacagcccac gggaagtggt
    36961 agcgcgcgtc aaaaccattt tgcgccgttg caaaccgcag cgtgagttgc agcaacagga
    37021 tgctgaaagc ccgttgatta tcgacgaagg tcgttttcag gcttcatggc gcgttaaaat
    37081 gcttgacctg acgcctgcgg aatttcgtct gctgaaaacg ctctctcatg aaccaggaaa
    37141 agtgttctcc cgcgagcaat tgctcaatca tctttatgac gactaccgcg tagtaaccga
    37201 ccgcaccatt gacagccaca ttaaaaacct gcgccgcaag ctggaatctc tcgacgccga
    37261 acagtcattt atccgcgccg tttacggcgt tggttaccgc tgggaagccg acgcctgccg
    37321 catcgtttag ttttagcgac atgactttgt tagccggaga tggcgttcag accagatccg
    37381 gcaacattat cccacgcatg gtcagcaaac tgacattatc tctccgccct ggcaggcatc
    37441 agaaaatcgc tcatacttta atcggtaaac agcaccttta gatgctgttt tcgatacaca
    37501 atttcaatca aggagtcatt atggctggtt ggtttgaact cagtaagagc agtgataatc
    37561 agttccggtt tgtgctaaaa gcgggcaatg gtgagactat cctcaccagc gagctgtata
    37621 cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg tagcaacagc ccgcaagaag
    37681 aacgctatga gaaaaaaacg gcaagtaacg gcaaattcta tttcaatctg aaagccgcta
    37741 atcatcaaat tatcggctcc agccagatgt acgccaccgc gcaatctcgt gaaaccggaa
    37801 ttgcctccgt taaagccaat ggcacaagcc agacggtgaa agacaatacg taattacgat
    37861 gccgggcgtg ggttgttggc gtccggcttt atatccaaat aacgcttagt tattaataat
    37921 taagagtaac cgccgccatt aaaatagtgg gcactgacga gcactcgttg tgctttcttt
    37981 gtttaccatc ccatacttat cctcctgaac atgttcatgt gccaaaatat cttctaatac
    38041 tttcgtgacg ctatccggtt tatgacatac actgatgtaa gatttttact tacaggcggg
    38101 cgtaaaatgg ccagaacgat gacggttgat ctcggcgatg agttgcgtga atttatcgag
    38161 tccttaattg aatccggcga ctatcgcacc caaagcgaag tgatccgtga gtcactgcga
    38221 ttacttcgag aaaagcaagc agagtctcgc ctgcaagcgt tacgtgacat gctcgcagaa
    38281 gggttgagta gtggtgaagc tcagccgtgg gaaaaggacg cgttcttacg gaaggtgaaa
    38341 gcagggataa ggaaataatg tgcgtattat taaactcatg ccaaaagcca atgaggattt
    38401 agaaggcatc tggtattaca gctatcacca tttcggcgaa ccacaagccg acagatatgt
    38461 ggaacatctt tcagacgttc tccagattct gagtaacaat aatataggta cgcccagacc
    38521 tgaattaggc gaaggtattt ttgttcttcc ttttgaacgc cacgtcattt atttcctgca
    38581 atcaccaggg gaaattatcg tcattcgtat ccttaaccaa aaccaggacg ccaccagaca
    38641 tttacactgg agctaaaccc gccgattgcg ctacaatgcc cgcccttaaa gtgggggcac
    38701 tcccctaacc gcttcatcag gtgaagcgga tctgacctgt catcagaacg agagaattat
    38761 gtttaaaccg gaactccttt ccccggcggg aacgctgaaa aatatgcgtt acgctttcgc
    38821 ttatggcgca gatgctgttt atgcgggcca gccgcgttat tccctgcgtg tgcgcaacaa
    38881 cgaattcaac cacgaaaatc ttcagctcgg catcaatgaa gcccacgcgc tggggaaaaa
    38941 gttttatgtc gtggtcaaca ttgcaccgca caacgccaag ctgaaaacct ttatccgtga
    39001 cctgaaaccg gtggtggaaa tggggccgga tgcgctgatt atgtccgatc cagggctgat
    39061 tatgctggtg cgtgagcact tccctgaaat gccgatccac ctttcggtgc aggctaacgc
    39121 cgtgaactgg gcgacggtga aattctggca gcaaatgggc ctgacccgcg tgatcctctc
    39181 tcgcgagctg tcgctggaag agattgaaga gatccgcaat caggtgccgg atatggagat
    39241 cgagatcttc gttcacggcg cgctgtgcat ggcctactcc ggtcgctgcc tgctctctgg
    39301 ctatatcaac aagcgcgacc cgaaccaggg cacctgcacc aacgcctgcc gctgggagta
    39361 caacgtccag gaagggaaag aagatgatgt tggcaacatc gtacacaagt acgaaccgat
    39421 tccggtgcaa aatgttgagc cgacgctggg tatcggcgcg ccaaccgaca aagtgtttat
    39481 gatcgaagaa gcccagcgtc cgggcgagta tatgaccgcg tttgaagatg agcacggcac
    39541 ttacatcatg aactcgaaag atctgcgcgc tatcgcccat gttgaacgcc tgaccaaaat
    39601 gggcgtgcat tcgctgaaaa tcgaaggtcg taccaaatct ttctactatt gtgcacgcac
    39661 cgcacaggtt taccgcaaag ctatcgatga cgccgctgcg ggaaaaccgt tcgataccag
    39721 cctgctggaa actctggaag gtctggcgca tcgtggctat accgaaggtt tcctgcgtcg
    39781 tcatactcac gacgattatc agaactacga atacggttat tcagtttctg accgccagca
    39841 gtttgttggt gagtttaccg gtgagcgcaa gggggacctc gcggcggtag cggtgaaaaa
    39901 taaattctcc gttggcgaca gccttgagct gatgacgccg caaggcaaca ttaattttac
    39961 ccttgagcac atggaaaacg ccaaaggcga agctatgccg atagcaccag gcgatggtta
    40021 tactgtgtgg ctcccggtcc cgcaggatct tgagctcaat tacgcgctgc tgatgcgtaa
    40081 tttctccggg gaaaccacgc gtaatcccca cggtaagtga ttaatttcga ttatttttcc
    40141 cggatggaaa attcttagaa accgatcaca tacagctgta tttattaagg ttatcatccg
    40201 tttcgctgaa aaacataacc cataaaatgc tagctgtacc aggaaccacc tccttagcct
    40261 gtgtaatctc ccttacacgg gcttattttt tatgcataag ccctatccct ggtcaccgtc
    40321 ttccattgac cacatcgata gaatctccct tcatagcacg atgcctttca cgtaacgtca
    40381 tcgtgctcgc acaggttccg gctaagcaca accagaacgc gcatgtttga cgcttaccaa
    40441 aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc
    40501 ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc
    40561 atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag aagcttcggc
    40621 ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa
    40681 tttaccgata cttgtctcat cattatgcac agcacaacca gacataataa aaatactaat
    40741 taatgatatt gcagctatct ttttcatctc accttccccc attaaatacc aacgacactc
    40801 tctagtgttt aaatataata atggcatgat tattataatt gaataggatt ataataaatg
    40861 ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct tcagggtaac
    40921 tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa tattaggtat
    40981 atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc cattgcaagc
    41041 tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct ccgactttca
    41101 tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc aatcataaag
    41161 gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg caaaagtact
    41221 gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat
    41281 gtgcgggtca cctgggagaa aggcgatgcc gcacgatatg tagaggaggc ccggaagttg
    41341 ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt tgctacggcg
    41401 ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt aggaaccgcc
    41461 aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct gaaactggca
    41521 attgccggta acgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt
    41581 aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa
    41641 gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa
    41701 ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt
    41761 ggtattggta acgggcgtca ggccggtggc ggtcagcaat tgtgtccgaa cgcgttaatt
    41821 aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc ggctctcgta
    41881 tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt
    41941 gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagcggacaa
    42001 aactttcata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcca
    42061 ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgaaat gccggatgcg
    42121 gtgtaaatac cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt
    42181 cttctgcaat gccagtttat ccacctctga tacagcatca tcggtaataa taatgtcaat
    42241 tttttcgatt ggcagtacct gattaaaacc gcgacggtta aatttcgatg aatcgagaac
    42301 ggcaatcact ttattagcgg cggtgaccat cgcaccgcta atcgaataac cttcattaaa
    42361 ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt
    42421 aatatcctgc agggaacgtt cagcaataga tccatgcata gatcgcgttt tatggcggac
    42481 cgtgccaccg cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgcagccgg
    42541 gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg cgataagcat
    42601 tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt tgactgccgc
    42661 ctgcgctatt ctgtttttgg gcgcgctggc gagctgaaat cgctctttaa acccgacttc
    42721 ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt
    42781 aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc
    42841 aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat
    42901 cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt
    42961 aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc
    43021 tggcccgcgg tttcagggaa tgagcaacac tttgcccggc atggcattac gagcgatgtc
    43081 acgcaccgcc tgggcgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct
    43141 taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct
    43201 ggagtagttc atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt
    43261 tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt gggcatgagg
    43321 accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag
    43381 ctgattaaag cgcagctcgc gtaaaacgcc ctgcatttgc ggcgcgctca tttcgctact
    43441 gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc
    43501 gatagccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca gcaggccaat
    43561 ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc
    43621 atgcagacca acggtaatcg gctcaataaa agccccatcc tcaataggca tatccgtggg
    43681 tagagcaaag acattttttc ggttaacgac aatatattcg gcaaatccac catcacgccg
    43741 cgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac actctggaca
    43801 agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta aatcatcaac
    43861 accggatcct accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata
    43921 atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat
    43981 ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg caacgcgcac
    44041 gacaccatca gtatcattca ctactgattt cataaaaact cctgattgtt aagggggata
    44101 acctccccct caggtaaaaa ttattctgcg agaacggctt tctcttgttt agtaaaacca
    44161 cgcgctctac gccaggtcat gaaaataccg gtcagataaa ttgcgccgat aataatgaaa
    44221 ccgggaatat tttgcgggga gaaaacctga atcagtaacc aggtaatggg agaaccgccc
    44281 tgatccattg aagccaccat acccccggct tttaacgccc cagcattagc cgccagttgg
    44341 gtgtgcaaac caatagtttg cgtcgcgatc cacagggtga tgctcataat gatgacaccc
    44401 gagattaagg tgcggaacag atttccacga tgcaccgcga ccgccatcgc cacgaagaag
    44461 ccgatagtgg caagatcgcc aaacggcagt acctgattac ccggaacaca aacagcaatt
    44521 aaaatggtga gcggaataaa aatcaggctt gccgatacca ccgccgtatg ccccagcagt
    44581 aatgcgggat ccaggccaat caggaactcc tgaccgccga acttcgcctg taaacgacta
    44641 cgggcctgct tagcgatggg cgttaaacca tccatgatgg gtttaatcac ccgtggcatc
    44701 agcagcatca ccgccgccgt ttttaccgcc agctgcaata cacctttgac atcgtaaccc
    44761 gcgaggatgc cgataatcag ccccatcaca aaacccacgg tgacaggctc gccaaacgga
    44821 ccaaatttac gctgaatatc gtcggcgcta aatttaattc ggttaacgcc tgggattttc
    44881 tcgatgatag tatcgaccag caccgcaatc ggccccatat acgccgacgt accgtgcgga
    44941 atagcaatgc cttccagctc aaagaaattt cgggtatcgc gggcaaacca gtcgccgagc
    45001 ttataaacaa acgccgcgtg aattaccaca cccgccatcc ctatcatcca tgaaccggtt
    45061 gccagatgca gcaacgcgcc ggtgaaggtc atatgccaga tattccagat atcaacattt
    45121 accacccgcg tcatacgggt cagtaacatc gccacgttaa ccagaatcgc aatcggaatc
    45181 gccaccagcg caatttgcga cgcccaggtc attggtgaag agcccggcca gccgacatcg
    45241 accacatgca gattcaggtc gaaattttcc gccatcgctt tcgccgccgg accaatggaa
    45301 tccagcatta agccaatcac aaggccaatg ccaacaaagc caatcccgat atgcagaccc
    45361 gctttaaagc aatcgcctgc cttcatgcct aatattttag aaaaaataat gatgacaatc
    45421 ggaagcatca ccgtagggcc gaggtcgaga atataacgca tgacttctga aaacataggt
    45481 cacccctgta agatagtcag aattttattt tgtaatgctt cgataccgac accagaaata
    45541 aaaggcatgc cgtgaactaa cggaatatcg ccaaaactgc gatccaccct ggcagtggta
    45601 catatcaaat gcacaccatc catataggtt tctatttcat taacccgaca ctggattaat
    45661 tcaacaggaa tattatgact ctgacacaac tctttaattt cttccgccgc catcgtagag
    45721 gtcgcaaccg cgcctccgca agcgacaata atcttgcgtt tcataaatag tcctttttat
    45781 tgttacagag ggatttattc gtctgaatct aaaacatact ttgtgaagta ttcctttaac
    45841 tgggtttcag gaagagtgat tagtgtctcg acgatatcgg gctgttgtaa cttgccaaat
    45901 aaacagcgta aaagtttcaa ttgctgctgc ggattttcca caatcaacgc aataaccaac
    45961 gataccgcca cgtcgttatc atcatccgct tgctgaaaat gaactttatt tgttggcctt
    46021 aacagataaa tggctgacga cttagcatga atcgcctcac aatgcggtat tgcaatggcg
    46081 tgctgctcaa gcattatccc ggtagggaat tctgcttctc tggcaattaa tgcctgtggc
    46141 caggtatcat gaaccacacc tttggcgagc atctcattac cgatatgggt taaaacttcg
    46201 ctacgatcga caaaagaaat tccgctacga acaaacaggt tagtcatata ccgtccttat
    46261 tccgcacagc cgtagcgata ggcgcgcaaa acatcataaa ttttatccat aatcagctga
    46321 tgcggtattg ctgataattc cccggactga atgcgttcaa attgtttggg aagatactga
    46381 ctaatcatgc ccagtgggat gtccacgcct tcaagattca ccatcatcgt ttcgacgcta
    46441 tttttaatcc gactatgcgg ccagtaataa cgaatacgat ccgacaggct gtaacgaata
    46501 tccagtaatg aatcgttaaa acccgtacga taatattttt tccagtattg cggttcgtcg
    46561 agcatcactt cttcaattac cgccaggcaa ccgctgcgat tttcaggggc gataagttcc
    46621 tgctcaattt gtgccagtgc aaatatcgcc tcgcgtaaag caaaggttaa tgcgggaccg
    46681 actttcaata ttgcaaagtg atcgcggact aattcccaat aagccgtccg ggtctggtaa
    46741 tcggtagaat gtgcttcata aaccattcgg gtgttttcta tccattgcgc cagcggctgc
    46801 gcttcctgcg gctgataatg gataatattg ctgtgatcaa attccacgcc cggctgcacc
    46861 acgatggcaa tcacgcgtgt taacgcctct gtcagcccac gggcaataaa ggccttttga
    46921 tgcgtacgta aagtattggc ggcatcttca acatgggtga tgtgtactga ctgaatggcg
    46981 ctggcctcac cgcccggaac cggaacttcg gtgccaatga catagctcag ttgctcacgc
    47041 tggcaatctg tcgccacact ttccgcagca aagcaaagca cagcagctcg ttccgcaacc
    47101 gtttctggtg ctaacggtat gggatccccc gcgcaggaca ttgacgcatc aagatgaatt
    47161 ttactgaagc cggcacgaac atattccttt accagctcga cggatttttc catcgccgca
    47221 tccgcatttt cttgctgcca gcagtttggc cccagatgat cgccgccgag aataatgcgt
    47281 tcgcgtgcaa acccaacttt atcggcaatc gtaaaaacaa attcgcgaaa gtctgccggt
    47341 gtcattccgg tataaccgcc aaattgattg acctggtttg acgttgcttc aatcagcact
    47401 ttgcgcgtgc tgttgcgatc aaatgccagc gccgcttcga taaccaacgg atgggcagaa
    47461 cagactgaac atatgccgat atgttcacca gctttatgcc gggcaattaa cgttttcatg
    47521 ttttttcctt gttaaatggc aggtgcgtta tgccctgccc tcgcagccac aatcggcaat
    47581 cactttgctc accacatcgc gcattgcgga tttagccgac tgcaaataat cccggggatc
    47641 ggtcgcttcg gggtgctcgg tcaggtaatt ttttaacgcc tgcgagaagg catttttcag
    47701 ctccgttgca acgttgattt tgcatatccc cactttgatg gtttgctgaa tatccttagt
    47761 cgataatcct gacgcgccat gcagcaccag cggtaagttc acccactggc gaatgttctc
    47821 cagtcgagaa aaatcaagca ccggtgcgct ggcatacata ccgtgagccg taccgatggc
    47881 gaccgccagg gaatcaatcc cggttgcctc ggcaaattca cgcgcctgtg cggggttggt
    47941 gtacaacgca tcggcttcat tgacttgcac atcatcttcc tggccgccaa gttgccccag
    48001 ctccgcctcg acgctgacat caaagcgatg gcaaaaatcc accacctctt tgacccgtga
    48061 aatattttgc gcaaaaggca aatgcgaggc gtcaatcatg actgagcgca cgccagaacg
    48121 aaccttctga gcgatatcgt caaatttcgt gtgatggtcg agatgaattg ccagtggatg
    48181 gtgatattgc ttcgccatcg cgctgaccag cgccaacaga ttttctgtac cagcatgagt
    48241 aaatgtgcca ggcgttccgg cgatgatgac cggcgcatgc aggttggcag cggtttccac
    48301 caccacttgc atcgtttcga gattgtgaat attgaatgcc ggaaccgcat aaccgccgcg
    48361 ctgtgcgttg ttcagcatct gctttgtcga t
//
LOCUS       DAZGXZ010000018        47132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000018 DAZGXZ010000000
VERSION     DAZGXZ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.018"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1116
                     /gene="proV"
                     /locus_tag="ACHKJF_001172"
     CDS             <1..1116
                     /gene="proV"
                     /locus_tag="ACHKJF_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP0948572.1"
                     /translation="SKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSGSGKSTMVRL
                     LNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMTVLDNTAFGM
                     ELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALAINPDILLMD
                     EAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGDRIAIMQNGEVVQVG
                     TPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGPRSALKLLQD
                     EDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQTPLSELLSHV
                     GQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
     gene            1109..2173
                     /gene="proW"
                     /locus_tag="ACHKJF_001173"
     CDS             1109..2173
                     /gene="proW"
                     /locus_tag="ACHKJF_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP0948573.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGMATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDTMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2231..3223
                     /gene="proX"
                     /locus_tag="ACHKJF_001174"
     CDS             2231..3223
                     /gene="proX"
                     /locus_tag="ACHKJF_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP0948574.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            3514..4698
                     /locus_tag="ACHKJF_001175"
     CDS             3514..4698
                     /locus_tag="ACHKJF_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0948575.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            4822..5559
                     /gene="ygaZ"
                     /locus_tag="ACHKJF_001176"
     CDS             4822..5559
                     /gene="ygaZ"
                     /locus_tag="ACHKJF_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP0948576.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            5549..5884
                     /gene="ygaH"
                     /locus_tag="ACHKJF_001177"
     CDS             5549..5884
                     /gene="ygaH"
                     /locus_tag="ACHKJF_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP0948577.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALTYG
                     LVWKVMAII"
     gene            5975..6505
                     /gene="emrR"
                     /locus_tag="ACHKJF_001178"
     CDS             5975..6505
                     /gene="emrR"
                     /locus_tag="ACHKJF_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP0948578.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            6632..7804
                     /gene="emrA"
                     /locus_tag="ACHKJF_001179"
     CDS             6632..7804
                     /gene="emrA"
                     /locus_tag="ACHKJF_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP0948579.1"
                     /translation="MSANAETQTLQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            7821..9359
                     /gene="emrB"
                     /locus_tag="ACHKJF_001180"
     CDS             7821..9359
                     /gene="emrB"
                     /locus_tag="ACHKJF_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP0948580.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9423..9938)
                     /gene="luxS"
                     /locus_tag="ACHKJF_001181"
     CDS             complement(9423..9938)
                     /gene="luxS"
                     /locus_tag="ACHKJF_001181"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP0948581.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10088..11644)
                     /gene="gshA"
                     /locus_tag="ACHKJF_001182"
     CDS             complement(10088..11644)
                     /gene="gshA"
                     /locus_tag="ACHKJF_001182"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP0948582.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(11717..12145)
                     /gene="yqaA"
                     /locus_tag="ACHKJF_001183"
     CDS             complement(11717..12145)
                     /gene="yqaA"
                     /locus_tag="ACHKJF_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP0948583.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12142..12708)
                     /gene="yqaB"
                     /locus_tag="ACHKJF_001184"
     CDS             complement(12142..12708)
                     /gene="yqaB"
                     /locus_tag="ACHKJF_001184"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP0948584.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(12989..13065)
                     /locus_tag="ACHKJF_001185"
     tRNA            complement(12989..13065)
                     /locus_tag="ACHKJF_001185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13029..13031),aa:Arg,seq:acg)
     gene            complement(13264..13340)
                     /locus_tag="ACHKJF_001186"
     tRNA            complement(13264..13340)
                     /locus_tag="ACHKJF_001186"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13304..13306),aa:Arg,seq:acg)
     gene            complement(13404..13480)
                     /locus_tag="ACHKJF_001187"
     tRNA            complement(13404..13480)
                     /locus_tag="ACHKJF_001187"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13444..13446),aa:Arg,seq:acg)
     gene            complement(13678..13754)
                     /locus_tag="ACHKJF_001188"
     tRNA            complement(13678..13754)
                     /locus_tag="ACHKJF_001188"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13718..13720),aa:Arg,seq:acg)
     gene            complement(13758..13850)
                     /locus_tag="ACHKJF_001189"
     tRNA            complement(13758..13850)
                     /locus_tag="ACHKJF_001189"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13814..13816),aa:Ser,seq:gct)
     gene            complement(14166..14351)
                     /gene="csrA"
                     /locus_tag="ACHKJF_001190"
     CDS             complement(14166..14351)
                     /gene="csrA"
                     /locus_tag="ACHKJF_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP0948585.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14586..17216)
                     /gene="alaS"
                     /locus_tag="ACHKJF_001191"
     CDS             complement(14586..17216)
                     /gene="alaS"
                     /locus_tag="ACHKJF_001191"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP0948586.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17344..17844)
                     /gene="recX"
                     /locus_tag="ACHKJF_001192"
     CDS             complement(17344..17844)
                     /gene="recX"
                     /locus_tag="ACHKJF_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP0948587.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18087..19148)
                     /gene="recA"
                     /locus_tag="ACHKJF_001193"
     CDS             complement(18087..19148)
                     /gene="recA"
                     /locus_tag="ACHKJF_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0948588.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19228..19725)
                     /gene="pncC"
                     /locus_tag="ACHKJF_001194"
     CDS             complement(19228..19725)
                     /gene="pncC"
                     /locus_tag="ACHKJF_001194"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP0948589.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(19870..20955)
                     /gene="mltB"
                     /locus_tag="ACHKJF_001195"
     CDS             complement(19870..20955)
                     /gene="mltB"
                     /locus_tag="ACHKJF_001195"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP0948590.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21211..21774
                     /gene="srlA"
                     /locus_tag="ACHKJF_001196"
                     /gene_synonym="gutA"
     CDS             21211..21774
                     /gene="srlA"
                     /locus_tag="ACHKJF_001196"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP0948591.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21771..22730
                     /gene="srlE"
                     /locus_tag="ACHKJF_001197"
                     /gene_synonym="gutE"
     CDS             21771..22730
                     /gene="srlE"
                     /locus_tag="ACHKJF_001197"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP0948592.1"
                     /translation="MTHIQIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22741..23112
                     /gene="srlB"
                     /locus_tag="ACHKJF_001198"
                     /gene_synonym="gutB"
     CDS             22741..23112
                     /gene="srlB"
                     /locus_tag="ACHKJF_001198"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP0948593.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23116..23895
                     /gene="srlD"
                     /locus_tag="ACHKJF_001199"
     CDS             23116..23895
                     /gene="srlD"
                     /locus_tag="ACHKJF_001199"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP0948594.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24000..24359
                     /gene="gutM"
                     /locus_tag="ACHKJF_001200"
     CDS             24000..24359
                     /gene="gutM"
                     /locus_tag="ACHKJF_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP0948595.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            24426..25199
                     /gene="srlR"
                     /locus_tag="ACHKJF_001201"
                     /gene_synonym="gutR"
     CDS             24426..25199
                     /gene="srlR"
                     /locus_tag="ACHKJF_001201"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP0948596.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25192..26157
                     /gene="gutQ"
                     /locus_tag="ACHKJF_001202"
     CDS             25192..26157
                     /gene="gutQ"
                     /locus_tag="ACHKJF_001202"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP0948597.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQTRGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26154..27668)
                     /gene="norR"
                     /locus_tag="ACHKJF_001203"
     CDS             complement(26154..27668)
                     /gene="norR"
                     /locus_tag="ACHKJF_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP0948598.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            27855..29294
                     /gene="norV"
                     /locus_tag="ACHKJF_001204"
     CDS             27855..29294
                     /gene="norV"
                     /locus_tag="ACHKJF_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP0948599.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29291..30424
                     /gene="norW"
                     /locus_tag="ACHKJF_001205"
     CDS             29291..30424
                     /gene="norW"
                     /locus_tag="ACHKJF_001205"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP0948600.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(30650..32902)
                     /gene="hypF"
                     /locus_tag="ACHKJF_001206"
     CDS             complement(30650..32902)
                     /gene="hypF"
                     /locus_tag="ACHKJF_001206"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP0948601.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33055..33582)
                     /gene="hydN"
                     /locus_tag="ACHKJF_001207"
     CDS             complement(33055..33582)
                     /gene="hydN"
                     /locus_tag="ACHKJF_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP0948602.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33731..34741)
                     /gene="ascG"
                     /locus_tag="ACHKJF_001208"
     CDS             complement(33731..34741)
                     /gene="ascG"
                     /locus_tag="ACHKJF_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP0948603.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            35001..36458
                     /gene="ascF"
                     /locus_tag="ACHKJF_001209"
     CDS             35001..36458
                     /gene="ascF"
                     /locus_tag="ACHKJF_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP0948604.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            36467..37891
                     /gene="ascB"
                     /locus_tag="ACHKJF_001210"
     CDS             36467..37891
                     /gene="ascB"
                     /locus_tag="ACHKJF_001210"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP0948605.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVELFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38005..38424)
                     /locus_tag="ACHKJF_001211"
     CDS             complement(38005..38424)
                     /locus_tag="ACHKJF_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0948606.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(38495..39250)
                     /locus_tag="ACHKJF_001212"
     CDS             complement(38495..39250)
                     /locus_tag="ACHKJF_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948607.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(39293..39763)
                     /gene="hycI"
                     /locus_tag="ACHKJF_001213"
     CDS             complement(39293..39763)
                     /gene="hycI"
                     /locus_tag="ACHKJF_001213"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP0948608.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(39756..40166)
                     /gene="hycH"
                     /locus_tag="ACHKJF_001214"
     CDS             complement(39756..40166)
                     /gene="hycH"
                     /locus_tag="ACHKJF_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP0948609.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(40163..40930)
                     /gene="hycG"
                     /locus_tag="ACHKJF_001215"
     CDS             complement(40163..40930)
                     /gene="hycG"
                     /locus_tag="ACHKJF_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP0948610.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(40930..41472)
                     /gene="hycF"
                     /locus_tag="ACHKJF_001216"
     CDS             complement(40930..41472)
                     /gene="hycF"
                     /locus_tag="ACHKJF_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP0948611.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(41482..43191)
                     /gene="hycE"
                     /locus_tag="ACHKJF_001217"
     CDS             complement(41482..43191)
                     /gene="hycE"
                     /locus_tag="ACHKJF_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP0948612.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAEIRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43209..44132)
                     /gene="hycD"
                     /locus_tag="ACHKJF_001218"
     CDS             complement(43209..44132)
                     /gene="hycD"
                     /locus_tag="ACHKJF_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP0948613.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVSGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44135..45961)
                     /gene="hycC"
                     /locus_tag="ACHKJF_001219"
     CDS             complement(44135..45961)
                     /gene="hycC"
                     /locus_tag="ACHKJF_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP0948614.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVTRLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(45958..46569)
                     /gene="hycB"
                     /locus_tag="ACHKJF_001220"
     CDS             complement(45958..46569)
                     /gene="hycB"
                     /locus_tag="ACHKJF_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP0948615.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSVDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(46694..>47132)
                     /gene="hycA"
                     /locus_tag="ACHKJF_001221"
     CDS             complement(46694..>47132)
                     /gene="hycA"
                     /locus_tag="ACHKJF_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP0948616.1"
                     /translation="KADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHHAMSCAPDK
                     ELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGMIDGKVNIR
                     DREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
BASE COUNT        11005 a        12972 c        12145 g        11010 t
ORIGIN      
        1 tcgaaagaac aaattctgga aaaaactggg ctatcgcttg gcgtaaaaga cgccagtctg
       61 gccattgaag aaggcgagat atttgtcatc atgggattat ccggctcggg taaatccaca
      121 atggtacgcc ttctcaatcg cctgattgaa cccacccgcg ggcaagtgct gattgatggt
      181 gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg tgcgcagaaa aaagattgcg
      241 atggtcttcc agtcctttgc cttaatgccg catatgaccg tgctggacaa tactgcgttc
      301 ggtatggaat tggccggaat taatgccgaa gaacgccggg aaaaagccct tgatgcactg
      361 cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg atgaactctc tggcgggatg
      421 cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc cggatatatt attaatggac
      481 gaagccttct cggcgctcga tccattaatt cgcaccgaga tgcaggatga gctggtaaaa
      541 ttacaggcga aacatcagcg caccattgtc tttatttccc acgatctcga tgaagctatg
      601 cgtattggcg accgaattgc cattatgcaa aatggtgaag tggtacaggt cggcacaccg
      661 gatgaaattc tcaataatcc ggcgaatgat tatgtccgta ccttcttccg tggcgttgat
      721 attagtcagg tattcagtgc gaaagatatt gcccgccgga caccgaatgg cttaattcgt
      781 aaaacccctg gcttcggccc acgttcggca ctgaaattat tgcaggatga agatcgcgaa
      841 tatggctacg ttatcgaacg cggtaataag tttgtcggcg cagtttccat cgattcgctt
      901 aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc tgattgatgc gccgttagca
      961 gtcgatgcac aaacgcctct tagcgagttg ctctctcatg tcggacaggc accctgtgcg
     1021 gtgcccgtgg tcgacgagga ccaacagtat gtcggcatca tttcgaaagg aatgctgctg
     1081 cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa aataatccgt gggataccac
     1141 gccagcggcg gacagtgccg tgcaatccgc agacgcctgg ggtacaccgg cgactgcacc
     1201 gactgacggc ggtggtgctg actggctgac cagtacgcct gcgccaaacg tcgagcattt
     1261 taatattctc gatccgttcc ataaaacgct gatcccgctc gacagttggg tcactgaagg
     1321 gatcgactgg gttgttaccc atttccgtcc cgtcttccag ggcgtgcgcg ttccggttga
     1381 ttacattctc aacggtttcc agcaattgct gctgggtatg cccgcaccgg tggcgattat
     1441 cgttttcgct ctcatcgcct ggcagatttc cggggtcgga atgggcatgg cgacgctggt
     1501 ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag gccatggtta ccctggcgct
     1561 ggtgttaacc gccctgctgt tctgtatcgt cattggtttg ccgctgggga tctggctggc
     1621 gagaagtccg cgagcggcga aaattattcg tccactgctt gataccatgc agaccacgcc
     1681 agcgtttgtt tatctggtgc caatcgtcat gctatttggt atcggtaacg tgccgggcgt
     1741 ggtggtgacg atcatctttg ctctgccgcc gattatccgt ctgaccattc tggggattaa
     1801 ccaggttccg gcggatctga ttgaagcctc gcgctcattc ggtgccagcc cgcgccagat
     1861 gctgttcaaa gttcagttac cgctggcgat gccgaccatt atggcgggcg ttaaccagac
     1921 gctgatgctg gccctttcta tggtggtcat tgcctcgatg attgccgtcg gcgggctggg
     1981 tcagatggta cttcgcggta tcggtcgtct ggatatgggg cttgccaccg ttggcggcgt
     2041 cgggattgtg atcctcgcca ttatcctcga tcgcctgacg caggccgttg ggcgcgactc
     2101 acgcagtcgc ggcaaccgtc gctggtacac cactggccct gttggtctgc tgacccgccc
     2161 attcattaag taactctgca cttgcccggt gacgccgggc attatcaccc tgccaaaaaa
     2221 aggaataaca atgcgacata gcgtactttt tgcgacagcg tttgccacgc ttatctctac
     2281 acaaactttt gctgccgatc tgccgggcaa aggtattact gtaaatccag ttcaaagcac
     2341 catcaccgaa gaaaccttcc agacgctgct ggtcagccgt gcgctggaga aattaggcta
     2401 taccgtcaac aaacccagcg aagtagatta caacgttggc tacacctcgc ttgcttccgg
     2461 cgatgcaacc ttcaccgccg tgaactggac gccactgcat gacaacatgt acgaagctgc
     2521 cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt aacggcgcgg cacagggtta
     2581 cctgatcgat aagaaaaccg ccgaccagta taaaatcacc aacatcgcac aactgaaaga
     2641 tccgaagatc gccaaactgt tcgataccaa cggcgacggt aaagcggatt taaccggttg
     2701 taaccctggc tggggctgcg aaggtgcgat caaccaccag cttgccgcgt atgaactgac
     2761 caacaccgtg acgcataatc aggggaacta cgcagcgatg atggccgaca ccatcagtcg
     2821 ctacaaagag ggcaaaccgg tgttttatta cacctggacg ccgtactggg tgagtaatga
     2881 gctgaagcca gggaaagatg tggtctggtt gcaggtgccg ttctccgcac tgccgggcga
     2941 taaaaatgcc gataccaaac tgccgaatgg cgcgaattac ggcttcccgg tcagcaccat
     3001 gcatatcgtt gccaacaaag cctggaccga gaaaaatccg gcggcagcca aactgtttgc
     3061 cattatgcag ttgccagtgg ccgatattaa cgcccagaac gccattatgc atgacggcaa
     3121 agcctcagaa ggcgatattc agggccacgt tgatggctgg atcaaagccc accagcagca
     3181 gttcgatggc tgggtgaatg aggcgctggc agcgcagaag taatttttat tcaggcggat
     3241 aaggcgtttt tgcctcatcc gccgctctgt acacaatgcc tgatgcgacg ctgacgcgtc
     3301 ttatcatgcc tacaaaatac actcattcgt atggcggata aggcgttttc gccgcatccg
     3361 ccgttctgtg cacaatgcct gatgcgacgc tggcgcgtct tatcatgcct acaaaccaca
     3421 tcgcacattt caacaatcta tctttcatcc catattcatc aacatccgct attattgatt
     3481 tccagcttaa tcatcacctg atgaacaaaa ataatgacta aacctaatca tgagcttagc
     3541 ccggcgctga tcgtgctgat gtctatcgcc accggtctgg cggtagccag taactattac
     3601 gcccagccat tgctcgacac catcgcgcgt aacttttccc tttccgccag ttcggcaggc
     3661 tttattgtta ccgccgcgca gttgggctat gccgcaggtc tactgtttct tgttcccctc
     3721 ggtgatatgt ttgaacgccg ccgcctgatt gtctcgatga ccttactggc agcgggcggt
     3781 atgttaatca ctgccagcag tcagtcactg gcgatgatga tcctcggtac ggcattaacc
     3841 ggtttattct cagtcgtggc acaaattctg gttccgctgg cagcgacgct ggcttcaccg
     3901 gacaaacgcg gcaaagtggt tggcactatt atgagcggtc tgctgttggg gatcttactc
     3961 gcacggaccg ttgccggatt gctggcgaat ctcggcggct ggcgcaccgt cttttgggtt
     4021 gcctcggtgt taatggcact gatggcgctg gcattatggc gtggtctgcc acaaatgaaa
     4081 tcagaaaccc acctcaacta cccacagttg ttaggttccg ttttcagcat gtttatcagc
     4141 gataagatcc tgcgcacccg cgcattgctg ggctgcctga cctttgccaa cttcagcatt
     4201 ctctggacct caatggcctt tttgcttgcc gctccacctt ttaactacag cgatggtgtc
     4261 attggtctgt ttggacttgc gggagctgcc ggagcgttgg gcgctcgtcc ggcgggcggt
     4321 tttgccgata agggtaaatc gcaccacacc acaactttcg gtctgctgct gctattactt
     4381 tcatggctgg cgatctggtt tggtcacact tccgtactgg cgctgattat cggcatcctg
     4441 gtgctggatc tcaccgtaca gggcgtgcat atcactaacc agacggtaat ttatcgaata
     4501 caccctgatg cgcgtaatcg cctgaccgca ggttacatga ccagctactt tattggcggt
     4561 gccgccggtt cgctaatttc agcctcagcc tggcaacatg gcggttgggc tggcgtttgt
     4621 ctggctggcg cgacgattgc cctggttaac ttactggtct ggtggcgagg ttttcatcgt
     4681 caggaagccg caaattaagc aatcagcaac cgttttcgtg gctttacact tataagggtg
     4741 ttaagaagcc catcagtctg ataaggttaa gatattcatt cagtctattt ataatattaa
     4801 caatcgttaa gcgtacactc tatggaaagc cctactccac agcctgctcc tggttcggcg
     4861 accttcatgg aaggatgcaa agacagttta ccgattgtta ttagttatat tccggtggcc
     4921 tttgcgttcg gtctgaatgc gacccgtctg ggattctctc ctctcgaaag cgtttttttc
     4981 tcctgcatca tttatgcagg cgcgagccag ttcgtcatta ccgcgatgct ggcagccggg
     5041 agtagtttgt gggttgctgc actgaccgtc atggcaatgg atgttcgcca tgtgttgtat
     5101 ggcccgtcac tgcgtagccg tattattcag cgtctgcaaa aatcgaaaac cgccctgtgg
     5161 gcgtttggcc tgacggatga ggtttttgcc gccgcaaccg caaaactggt acgcaataat
     5221 cgccgctgga gcgagaactg gatgatcggc attgccttca gttcatggtc atcgtgggta
     5281 tttggtacgg taataggggc attctccggc agcggcttgc tgcaaggtta tcccgccgtt
     5341 gaagcagcat taggttttat gcttccggca ctctttatga gtttcctgct cgcctctttc
     5401 cagcgcaaac aatctctttg cgttaccgca gcgttagttg gtgcccttgc aggcgtaacg
     5461 ctattttcta ttcccgtcgc cattctggca ggcattgtct gtggctgcct cactgcgtta
     5521 atccaggcat tctggcaagg agcgcccgat gagctatgag gttctgctgc ttgggttact
     5581 ggttggcgcg gcgaattatt gcttccgcta tttgccgctg cgcctgcgtg tgggtaatgc
     5641 ccgcccaacc aaacgtggcg cggtaggtat tttgctcgac accattggca tcgcctcgat
     5701 atgcgctctg ctggttgtct ctaccgcacc agaagtgatg cacgatacac gccgtttcgt
     5761 gcccacgctg gtcggcttcg cggtactggg tgccagtttc tataaaacac gcagcattat
     5821 catcccaaca ctgcttagtg cgctgaccta cgggctcgtc tggaaagtga tggcgattat
     5881 ataatccaca agaatcattt ttctaaaaca atacatttac tttatttgtc actgtcgtta
     5941 ctatatcggc tgaaattaat gaggtcatac ccaaatggat agttcgttta cgcccattga
     6001 acaaatgcta aaatttcgcg ccagccgcca cgaagatttt ccttatcagg agatccttct
     6061 gactcgtctt tgcatgcaca tgcaaagcaa gctgctggag aaccgcaata aaatgctgaa
     6121 ggctcagggg attaacgaga cgttgtttat ggcgttgatt acgctggagt ctcaggaaaa
     6181 ccacagtatt cagccttctg aattaagttg tgctcttgga tcatcccgta ccaacgcgac
     6241 gcgtattgcc gatgaactgg aaaaacgcgg ttggatcgaa cgtcgtgaaa gcgataacga
     6301 tcgccgctgc ctgcatctgc aattaacgga aaaaggtcac gagtttttgc gcgaggtttt
     6361 accaccgcag cataactgcc tgcatcaact ctggtccgcg ctcagcacaa cagaaaaaga
     6421 tcagctcgag caaatcaccc gcaaattgct ctcccgtctc gaccagatgg aacaagacgg
     6481 tgtggttctc gaagcgatga gctaacgcgt catctcgctc aaaaatccag atttataaaa
     6541 gaaaaaatga ctggccagca tcgcaacatg ctggcctttt tggcaagcag gtcggctcag
     6601 ccgatgagtt aagaagatcg tggagaacaa tatgagcgca aatgcggaga ctcaaaccct
     6661 gcagcaaccg gtaaagaaga gcggcaaacg taagcgtctg ctcctccttc tcaccttgct
     6721 ctttataatt attgccgtag cgatagggat ttattggttt ttggtactgc gtcatttcga
     6781 agaaaccgat gacgcatacg tggcagggaa tcagattcaa attatgtctc aggtgtctgg
     6841 cagcgtgacg aaagtctggg ccgataacac cgattttgta aaagaaggcg atgttctggt
     6901 cactctcgac ccgacagatg ctcgccaggc gtttgaaaaa gccaaaactg cactggcttc
     6961 cagcgttcgc caaacccacc agctgatgat taacagcaag cagttgcagg cgaatattga
     7021 ggtgcagaaa atcgccctcg cgaaagcaca aagcgactac aaccgccgtg tgccgctggg
     7081 caatgccaac ctgattggtc gcgaagagct gcaacacgcc cgcgacgccg tcaccagtgc
     7141 ccaggcgcaa ctggacgtcg ctattcaaca atacaatgcc aatcaggcga tgattctggg
     7201 gactaaactg gaagatcagc ctgccgtgca acaggctgcc accgaagtac gtaacgcctg
     7261 gctggcgctg gagcgtaccc gtattgtcag tccgatgact ggttatgttt cccgtcgtgc
     7321 cgtgcaaccg ggtgcgcaaa ttagcccaac gacgccgctg atggcggtcg ttccagccac
     7381 caatatgtgg gtggatgcca actttaaaga gacgcagatt gccaatatgc gcatcggtca
     7441 gccggtcacc atcaccaccg atatttacgg cgatgatgtg aaatacaccg gtaaagtggt
     7501 tggtctggat atgggcacag gtagcgcgtt ctcactgctt ccggcgcaaa atgcgaccgg
     7561 taactggatc aaagtcgttc agcgtctacc tgtgcgtatc gaactggacc agaaacagct
     7621 ggagcaatac ccgctgcgca ttggtttgtc cacgctggtg agcgtcaata ccactaaccg
     7681 tgacggtcag gtactggcaa ataaagtacg ttcaacaccg gtcgcggtaa gtaccgcgcg
     7741 tgaaatcagc ctggcacctg tcaataaact gatcgacgat atcgtaaaag ctaacgctgg
     7801 ctaatccaga ggtgcgtgtg atgcaacagc aaaaaccgct ggaaggcgcg caactggtca
     7861 ttatgacgat tgcgctgtca ctggcgacat tcatgcaggt gctggactcc accattgcta
     7921 acgtggcgat ccccactatc gccgggaatc tgggctcatc gctcagccag ggaacgtggg
     7981 taatcacttc tttcggggtg gcgaatgcca tctcgatccc gcttaccggc tggctggcaa
     8041 agcgcgtcgg ggaagtgaaa ctgttccttt ggtccaccat cgcctttgct attgcgtcgt
     8101 gggcgtgtgg tgtctccagc agcctgaata tgctgatctt cttccgcgtg attcagggga
     8161 ttgtcgccgg gccgttgatc ccgctttcgc aaagtctatt gctgaataac tacccgccag
     8221 ccaaacgctc gatcgcgctg gcgttgtggt cgatgacggt gattgtcgcg ccaatttgcg
     8281 gccctatcct cggcggttat atcagcgata attaccactg gggctggata ttcttcatca
     8341 acgtgccgat tggcgtggcg gtggtgttga tgacactgca aactctgcgc ggacgtgaaa
     8401 cccgcaccga acggcggcgg attgatgccg tggggctggc actgctggtt attggtatcg
     8461 gcagcctgca gattatgctc gaccgcggta aagagctgga ctggttttca tcacaggaaa
     8521 ttatcattct taccgtggtg gcggtggtgg ctatctgctt cctgattgtc tgggagctga
     8581 ccgacgataa cccgatagtc gatctgtcgt tgtttaagtc gcgcaacttc accatcggct
     8641 gcttgtgtat cagcctcgcg tatatgctct acttcggcgc tattgttctg ctgccgcagt
     8701 tgttgcagga ggtctacggt tacacggcga cctgggcagg tttggcctct gcgccggtag
     8761 ggattattcc ggtgatcctg tcgccgatta tcggccgctt cgcgcataaa ctggatatgc
     8821 ggcggctggt aaccttcagc tttattatgt atgccgtctg cttctactgg cgtgcctata
     8881 cctttgaacc aggtatggat tttggcgcgt cggcctggcc gcagtttatc caggggtttg
     8941 cggtggcctg cttctttatg ccgctgacca ccattacgct gtctggtttg ccaccggaac
     9001 gactggcggc ggcatcgagc ctctctaact ttacgcgaac gctggcgggg tctatcggca
     9061 cgtcgataac cacgaccatg tggaccaacc gcgagtcgat gcaccatgcg cagttgactg
     9121 agtcggtaaa cccgttcaac ccgaatgccc aggcgatgta cagtcaactg gaagggcttg
     9181 ggatgacgca acagcaggca tcaggctgga ttgcccagca gatcaccaat caggggctga
     9241 ttatttccgc caatgagatc ttctggatgt cagccgggat attcctcgta ctgctggggc
     9301 tggtgtggtt tgctaaaccg ccatttggcg caggtggcgg cggaggcggt gcgcactaag
     9361 tacaactaag ccagttcatt tgaactggct tttttcaatt aattgtgaag atagtttact
     9421 gactagatgt gcagttcctg caacttctct ttcggcagtg ccagttcttc gttgctgttg
     9481 atgcgtacgt cacgttccag aatgctacgc gcaatatcct gcgcttcctg caacgagtgc
     9541 atctggtaag tgccacactg gtagacattc agttccggga tctgattctg atcctgcact
     9601 ttcagcacgt cttccattgc cgctttccag gcatcagcaa cacgctgctc atctggcgta
     9661 ccaatcagac tcatataaaa accggtgcgg cagcccattg gcgagatatc gataatctct
     9721 acacccttac cgttaagatg gttacgcata aaaccagcaa acaggtgctc cagggtatgg
     9781 atccctcttt ctggcatcac ttctttgttc ggcacgcaga agcgcagatc gaacacggtg
     9841 attgcgtcgc catgcggggt gttcattgtt ttcgccaccc gaactgcagg cgcttccatc
     9901 cgggtatgat cgactgtgaa gctatctaac aacggcattt agccacctcc ggtaattttt
     9961 tttaaaattt tctgaactct ttcttcccag gcgagtctga gtatatgaaa gacgcgcatt
    10021 tgttatcatc atccctgaat tcagagatga aattttggcc actcacgagt ggcctttttc
    10081 ttttctgtca ggcgtgtttt tccagccaca ccgcaaacgg ttcggtatca gcggtttcca
    10141 tttcctgctg acggcgttca gatgcctcgc gctcggctac aaaatcctct tcgcgcagaa
    10201 tttccagcgg ctcttcacgc agcagattac ggtaggcttc tgcaaacgct ttgcctgttc
    10261 cgccaatacc agtatcaatc atagacctta agatacgggc agagaaagtc agatcgggat
    10321 tatcgaagca ggcaaccagt tcatcacaca ctttctgata cgcttcgccg ccgttaatgc
    10381 tatccagcgt ttgcgcgacg cgtttcagat cgcggaacag atctttaccc acctgcagta
    10441 acgggaactg cgcggtttcg cagccgatac ccagcgtcag gcccggtttg cgaccttcaa
    10501 ggatcacccg gttccagtta acgcgcgtac aggcaagttc gctactgctc atttccggtg
    10561 catcagccag cgcacaccag accataaaca ggtcgaggaa tcgcacctgc tgttcatcta
    10621 caccaatcgg cgagaacggg ttgatgtcca gcgaacgcac ttcaatatat tcaatgccgc
    10681 cacgtaacag cgcatcagaa ggtgactcgc cgctgcgggt aacgcgtttt ggacgaatcg
    10741 gcgcatacag ttcgttttca atctgcagca cgttgctatt gatttgcagc ctcttaccgt
    10801 ctttctcaat accaatcttc gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc
    10861 ccgctacgta ctcgtaaaga tcgttgaagg taataccaag attgctttgc gatttattgg
    10921 tatagccgag atcgctcaaa cgaagagagg tcgcatacgg caggtaatac ataccgcact
    10981 cggttttctc aaacggcagc gacgttggtt ttccttgcag gaaagaagaa caaatcgccg
    11041 gagatgcacc aaacagataa ggaatgaccc aaccgaaacg atagtaattg cggataacgc
    11101 ggaaatagcc cgcagaaatt ttctctttgg catcagcgcc cgagatatca ccgcacttcg
    11161 cttgccagaa tgccattggc aaagagaaat tgtagtgcac gccggaaatg gtttgcatca
    11221 gcgcgccgta gcgatttttc agcccttcac gatacagcgt tttaaagcgt ccggtgttag
    11281 aagtgccgta ctgtgccagt tcgatgtcct gaccttctgc gatgtagcat ggcatactta
    11341 acggccacat ccgctcatca cccatattgc gcgccgtata acgatgcaga tcgcgcataa
    11401 aggtcagcat atgttcaata tcaccatcca ctggtgtaat gaattccagc aatgcttccg
    11461 caaaatcggt agtaatccat ttgtgcgtca gtgcggaacc taatgcttca ggatgacctg
    11521 ttgttgccag tgtgccatca gcattaacac gcaaagtttc gcgctccagc ccacgctgta
    11581 tcccctttaa cgcctgagga tgtttttcca gccaggccag cgcctgtgat acgtccggga
    11641 tcaaattgac ctcccgcctg tcaaaatcgt tttaattagc ataactgtaa tggtgaccat
    11701 atgtgcaggc ctacaattag tgccaccaca tcatgccctg aacggtcgct gctgcaactg
    11761 caacatagcg taacgcttta ccaaggcaca aaaaaaagat tactggcccc caggagatgc
    11821 gcatccatcc cgctaacaga cacagtaaat cgccaaccac gggcatccag cttaataata
    11881 gcgtgactgc accatagcgt ttcagccagc cggtagcttt ctcttgccag cgcgatgttt
    11941 tacgcaatgg aaagaaacgc ccaaggataa cgttagttaa ccctccaagg ctattaccca
    12001 ttgttgctgt taaaactaaa acccagggat aactgatccc ggaaagcaac attgccacca
    12061 gcacgacttc ggagttgccg ggtaatagtg tagcgctgag aaaactactg gcaaacaacg
    12121 agaaaagcga taacgcttca ctcacagcaa gcgaacatcc acggcgtcca tgcctgctgc
    12181 acgggccgcc tgaataccga aatcggcatc ttcaaagacc acacactgcg tcggttgcac
    12241 gcccatacgc tgcgcgcaca acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg
    12301 atcagcagcg acgacggcgt caaaataacg gcgtaatccc aggtgcgcca gcaatgcctc
    12361 agcgatggcg ctttcactcc ccgttcctac agccattggg cgacgaccat gccaactttt
    12421 caccacttca acaagaggaa gcggctcgac actatccagc agcatacttc ttactgcttc
    12481 tgttttttca cgcgctaacg catgcgggtc gagatcggcc tgattcagct caataattgc
    12541 ctgagcaata cgccaggtgg gcgatccatt aagcgcaatc attgcctgaa catcgtactg
    12601 aagaccgtag tgccctaata cttcgcgcca cgctttacgg tgcgtaggct ccgtatccag
    12661 gattgtgcca tccatatcaa aaattaaacc tgcataacgc tcgtacatgg tcttctcgca
    12721 agtcgaaaaa tatgacgcta ctttagcgta attgcttgat tttgtcgctg atggcgaagg
    12781 agaagaaaat tcaggaaatg gataaagtag ttatttagaa atgagatatt tttgaaggaa
    12841 atttttggga gaagatggtg catccgggag gattactcac tggcgttcgc ccttcgggcc
    12901 gttgctgacg caacgttatc ctccctggtg cctgcggtta ataccgctat ctcttcgata
    12961 ccttcattgc tgaaggttac ggagaatatg gtgcatccgg gaggattcga acctccgacc
    13021 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg gaaaatttat
    13081 tttactgctg atattgatat cgctactaaa gccatatcaa gtaagagatg gtgcatccgg
    13141 gaggattact cgccttgcgg ctcgcccttc gggccgttgc taaagcaacg ttatcatccc
    13201 tggtgcctgc ggttaattcc gctacctctt cgataccttc attgctgaag atttcggaga
    13261 atatggtgca tccgggagga ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta
    13321 tccagctgag ctacggatgc atcgggaaaa tttattttac tgctgatatt gataccgcta
    13381 ctaaagccat atcaagtaag agatggtgca tccgggagga ttcgaacctc cgaccgctcg
    13441 gttcgtagcc gagtactcta tccagctgag ctacggatgc atcgggaaac ttactttact
    13501 gcagattttt tgataccgct actaaagccg tatcaagtaa gagatggtgc atccgggagg
    13561 attactcgcc ttgcggctcg cccttcgggc cgttgctaaa gcaacgttat cctccctggt
    13621 gcctgcggtt ataccgccac ctcttccata ccttcatcgc tgaaagtacg tagaatatgg
    13681 tgcatccggg aggattcgaa cctccgaccg ctcggttcgt agccgagtac tctatccagc
    13741 tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa ccccggatgc agcttttgac
    13801 cgcatactcc cttagcaggg gagcgccttc agcctctcgg ccacctcacc acacgcctct
    13861 tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc tgcgtggcgc acatattact
    13921 ttctgagact tataagtcaa acaatttttc ctacactttt atcgtttgca cacttcacgt
    13981 tcaattagtc tgtaaaaacg tcaaaaagag tgttttatca acagaaaaat ggaggtctga
    14041 cagatagtag taatgcaaaa aaatggagac ttaagttgaa tgaacgggag taaagcgaaa
    14101 agactataga gtgaaggaga aattttgagg gtgcgtctca ccgataaaga tgagacgcgg
    14161 aaagattagt aactggactg ctgggatttt tcagcctgga tacgctggta gatctcttca
    14221 cggtgaacag aaacttcctt cggggcattt acgccaatac gtacctggtt gccctttacc
    14281 cctaaaactg tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc
    14341 agaatcagca ttctttgctc cttgaaagat taaaagagtc gggtctctct gtatcccggc
    14401 attatccatc atataacgcc aaaaagtaag caatgacaaa cacattacat gtaagcagtc
    14461 atggcattac attctgttaa acctaagttt agccgatata cacaacttca acctgacttt
    14521 atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag tccacggcat tgcctgacgc
    14581 ttatattatt gcaatttcgc gctgacccag cctttcacac tggctaacgc tgcaggtaag
    14641 gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac gtccaccacc cttgccgccc
    14701 acctgctgag cgaccatacc aatcagttcc cctgctttca cacgatctgt gacgtcctta
    14761 gatacgcctg caatcagaga aaccttacct tcggctaccg ttgccagcac gataattgtc
    14821 gaccccagct gattttttaa atcgtcaacc atggtacgca acattttcgg ctcaacaccg
    14881 ctaagctcgc taaccaacag cttaacacca ttaacatcaa ttgccttact ggaaagattt
    14941 gcgctctcct gtgcggcagc ttgttcttta agctgttgta gttctttttc taactgacgc
    15001 gtacgttcca gtactgagcg cactttatcc gccagattat tgctatcgcc tttcagcaga
    15061 tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg tggtgatagc gccttctccg
    15121 gttaccgctt cgatacgacg aacgcctgca gcagtacccg attcagagat gatgcggaac
    15181 agaccaatat caccagtgcg gctggcgtga gtaccgccac acagctcggt ggagaaatcg
    15241 cccatgctca gcacgcgtac acgctcatca tacttctcgc cgaacagcgc catcgcacct
    15301 ttcgctttcg ccgcttcgag atccatgatg ttggtttcga tcggcaaatt gcgacgaatc
    15361 tgtgcgttca ccaggtcttc gaccgcacga atctcttccg gtttcatcgc ttcgttgtgt
    15421 gagaagtcga agcgcagcac cttgtcgtta accagtgaac ctttctgcga tacatgagtc
    15481 cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg cggagtgatt cagacgaata
    15541 cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt cgcccacttt cagagaaccc
    15601 gcagcaagtt taccgatgtg accaatcgcc tggccgtatt tctgcgtatc ttccaccgcg
    15661 aaggagaagt tagcgccttt cagttcgcct ttatcaccaa cctgaccgcc ggattccgca
    15721 tagaatggcg tttgatccag cacgaccaca gcttcctggc ctgcattgat ggcatcaacc
    15781 gctttaccat caacaaacag cgcggtcact ttgccgttca gttccagatg gtcatagcct
    15841 ttaaattcag atgcactatc aacacggatc attgcgttgt aatcggcacc aaagccgctg
    15901 gcttcgcgcg cacgacgacg ctgctcttcc attgctgctt caaaaccagc ttcgtcaact
    15961 ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa ccgggaagcc ataggtgtcg
    16021 tacagacgga aagccgtttc accatccagc gtatcaccag aaagttttgc cagctcttca
    16081 tccagcaacg ccagaccgcg ctccagagta cgggcaaact gctcttcttc agtcttcagc
    16141 acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac ccgcagagcc cataacgtcg
    16201 atcagcggac caaccagttt gtagaagaag gtttctttcg cgccgagcat attgccgtga
    16261 cgcactgcgc gacgaatgat acgacgcagt acataaccac ggttttcatt ggacggcatt
    16321 acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt cagcgattac gcgcagcgat
    16381 ttattgctca gatcggtcgc gccagtgact ttcgctaccg cctggatcaa cgtgcggaac
    16441 aggtcgatgt catagttaga gttaacgtgt tgcagcaccg cagcaatacg ctccagaccc
    16501 ataccggtat ctacagacgg cttcggcagc ggttccatcg tgccatctgc ctggcggttg
    16561 aactgcatga agacgatgtt ccagatctca atgtagcggt cgccgtcttc ttccggactt
    16621 cccggagggc caccccaaat gtggtcgccg tgatcgtaga agatttcggt gcacgggccg
    16681 cacggaccag tgtcacccat ctgccagaag ttgtcagatg cgtatggcgc acctttgtta
    16741 tcgccgatgc gaataatacg ttcgcgcggg atccctactt ctttttccca gatttcgtag
    16801 gcttcgtcgt cgctttcata gacggtaacc cacagacgct ctttcggcag ggcaaaccat
    16861 ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat cgtgtttgaa atagtcgccg
    16921 aagctgaagt tgcccagcat ttcgaagaag gtatggtgac gcgcggtgta accgacgttt
    16981 tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt gggaagtggt agcgcgggaa
    17041 taattacgct tgtcgagccc aaggaacaca tccttgaact ggttcatccc ggcgttggta
    17101 aacaacaaag ttgggtcgtt atggggtacc agggagctgc tggcaactac ctgatgtccc
    17161 ttactatgga aaaagtcgag aaacgcctga cggatctcag cggtgctctt gctcataatt
    17221 atcctgaaat caagctaacg aaatatcgcc accagctcca gcgtgtctta accgccgggc
    17281 tggtaactga aaagtgggaa taagataagt tttcttgact gggaagtaaa ataccgtatg
    17341 cgttcagtcg gcaaaatttc gccaaatatc ctggatatct tccatcagat agccacgata
    17401 gagcaaaaaa cgctggatct taactttttc tgaaaagaca gttggcaaag gttcaccata
    17461 ttttcgcgtc gcctgatcgc gcgccagtgc gcaccagtcg atgtcacatt cacgcatcgc
    17521 tttttctgtc gcttcgcggg aaataccttt ctgattcagt tcctggcgaa tacgcgcagg
    17581 tccataacct ttgcggctac ggctggcgat aaagcgcgca acaaatcggc tgtcatcgag
    17641 atagccatgt tcatggcacc aggcaataac gcgctcgtaa tcttctgccg tagcatcaat
    17701 ctcttctggg ccatttttgc ccataatcgg tgccgcgagt ttacgtcgca gttcttgctc
    17761 actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc aacaggcgag catatgccgg
    17821 gcgacgggac gttgattctg tcatggcata tccttacaac ttaaaaaagc aaaagggccg
    17881 cagatgcgac ccaggttttg ctgataaaca gatttaatag tcaatgagtg tgcagaatgt
    17941 tcccgcaggc ccaattcgtt agaggatcga ggtgagagta atttttcacg ccagtgctgg
    18001 cctgttcccc tcaccctaac cctctcccca aaggggcgag gggaccgtcc gtgcacacat
    18061 tatctattat catcaataac gaacgattaa aaatcttcgt tagtttctgc tacgccttcg
    18121 ctgtcatcta cagagaaatc cggcgttgag ttcgggttgc tcagcagcaa ctcacgtact
    18181 ttcttctcga tctctttcgc ggtttccggg ttatctttca gccaggcagt tgcattcgct
    18241 ttaccctgac cgatcttctc acctttgtag ctgtaccacg cgcctgcttt ctcgatcagc
    18301 ttctctttta cgcccaggtc aaccagttcg ccgtagaagt tgataccttc gccgtagagg
    18361 atctgaaatt cagcctgttt aaacggtgca gcgattttgt tcttcaccac tttcacgcgg
    18421 gtttcgctac ccaccacgtt ttcgccctct ttcaccgcgc cgatacgacg gatgtcgaga
    18481 cgaacagagg cgtagaattt cagcgcgtta ccaccggtag tggtttccgg gttaccgaac
    18541 atcacaccaa ttttcatacg gatctggttg atgaagatca gcagcgtgtt ggactgcttc
    18601 aggttacccg ccagcttacg catcgcctgg ctcatcatac gtgccgcaag gcccatgtga
    18661 gagtcgccga tttcgccttc gatttccgct ttcggcgtca gtgccgccac ggagtcaacg
    18721 acgataacgt ctactgcgcc agaacgcgcc agggcgtcac agatttccag tgcctgctcg
    18781 ccggtgtccg gctgggagca cagcaggtta tcgatatcga cgcccagttt acgtgcgtag
    18841 attgggtcca gcgcgtgttc agcatcgata aacgcacagg ttttaccttc acgctgcgct
    18901 gcggcgatca cctgcagcgt cagcgtggtt ttaccggaag attccggtcc gtagatttcg
    18961 acgatacggc ccatcggcag accacctgcc ccaagcgcga tatccagtga aagcgaaccg
    19021 gtagagatgg tttccacatc catggaacgg tcttcaccca ggcgcatgat ggagccttta
    19081 ccaaattgtt tctcaatctg gcccagtgct gccgccaacg ctttctgttt gttttcgtcg
    19141 atagccattt ttactcctgt catgccgggt aataccggat agtcaatatg ttctgttgaa
    19201 gcaattatac tgtatgctca tacagtatca agtgttttgt agaaattgtt gccacaaggt
    19261 ctgcaatgca tacgcagtag cctgacgacg caccgcatca cggtcaccgc tgaagcattc
    19321 ccgccgggta atgccttcac cgcgggcagt ggcaaaagca aaccagacgg tgccgacagg
    19381 cttctcttca ctgccgccat ccggcccggc gataccacta atagacacgg cataatcagc
    19441 acgagccgct ttcagtgcac ctatcgccat ttccaccacg acgggttcac tcaccgcgcc
    19501 atgctgcgcc agcgtctctt cgcgtacgcc gatcatctgc gctttggctt cgttactgta
    19561 ggtgacaaat ccgcgttcaa accaggcgga gctaccggca atatcggtaa tcactttcgc
    19621 tacccaacca ccggtacaag actcggcagt tgttacggtt gcgccacggg ctttcagcgc
    19681 ctgcccaacc tgttcactta actgcatcag ttcactgtca gtcatcacaa cctctgtcag
    19741 tcaaaaattt atgccggaca agatagcact ttcgtcagga agatggtgtt ggcgttgcaa
    19801 ttttacgagg aggattcaga aaaaagctga ttagccagag ggaagctcac gcccccctct
    19861 tgcaaatagt tactgcacgc gcgccagcgc cacggcttgt cctaactgcc agaccgccat
    19921 tgcgtaatgg gtgctgtggt tataacgggt aatggtgtag aagttcggca gaccgtacca
    19981 gtactggtaa ccggtaccga catccagacg cagcaggctg gcttgttgat ggttgcccag
    20041 cggctgctgt ggcgttaaac ctgccgtggc aagctgcgaa atgctgtacc tggttttgaa
    20101 gccatttggc aagcctggag cctgaccgtt tgccattacc gcgacctgat cgcctttcac
    20161 ccagccgtgc gctttgaaat agttcgccac gctaccgatc gcatcaaccg gatcccacag
    20221 gttgatatgc ccgtcgccgc tgaaatctac cgcatattgt ttgtaagacg acggcataaa
    20281 ctgtccgtag cccatcgccc cggcaaagga acctttcaga ttgagcggat cgtcctgctc
    20341 gtcgcgcgcc atcaacagga aggtttccag ctcgccagag aaatactccg cgcggcgtgg
    20401 gtagttaaat gacagcgttg ccagcgcatc gaggatgcga gttttcccca tcacgcgccc
    20461 ccagcgggtt tcaacgccga taatcccgac gataatttcc ggcggtacgc catacacctg
    20521 ccacgcgcga ttcaacgcat cttcatactg attccagaaa accacaccgt tctgcacgtt
    20581 gtccggcgta ataaattttt tgcgataacg gagccatgcg ccgttcggac ctgatggcgg
    20641 tttcaccgat gtggttggtg cctggttatc catcagccgc agtaccgaat ccagacgctt
    20701 cgcctgggag agaatttcct gcaactgctg acgatcgaaa ccgtgtttgt tcaccatttt
    20761 gtcgatgaac tgctgggcat tcgggttatt agcgaaatcg ccgcccatct gcatcacatt
    20821 gtgctgcggc tcaagcagga agccgccaga cggcgttccg gtggtcgttt cagtctcagt
    20881 aggttttggc ttgctgctac aggcggcaag caacacaaaa aggggaagca atgttacata
    20941 acgacgcttg aacatgaggg gtccatttaa cagattcaac caggggcaag tatggtaaag
    21001 catcacgccc cacacaagga agcggtagtc actgcccgat acggacttta cataactcaa
    21061 ctcattcccc tcgctatcct tttactcaaa ctttcaaatt aaaatattta tctttcattt
    21121 tgagatcaaa ataacacttt taaatctttc aatctgatta gattaggttg ccgtttggta
    21181 ataaaacaat aaatcctgaa ggagagaaca atgatagaaa ccattactca tggtgcagag
    21241 tggtttatcg ggctgttcca aaagggcgga gaggtgttta ccgggatggt gaccggcatt
    21301 cttccgctgt tgattagcct gctggttatc atgaacgcac tgattaattt tatcggtcag
    21361 catcgtattg aacgttttgc tcaacgttgc gccggtaacc ctgtttcccg ttacctactg
    21421 ttaccgtgca ttggcacgtt tgtcttttgc aatccgatga ccttaagcct gggtcgcttt
    21481 atgccggaaa agtacaaacc cagctactac gcggcggcct cttatagctg ccactcaatg
    21541 aatggcctct tcccccatat caaccctggc gaactgtttg tttatcttgg cattgccagc
    21601 ggtctgacaa cgctgaacct gccacttggc ccactggcgg tgagttatct gctggttggt
    21661 ctggtcacca atttcttccg cggctgggtg accgatctga ccaccgccat ttttgagaaa
    21721 aagatgggca ttcaacttga acaaaaagtt cacctggcag gagcaacatc atgacgcata
    21781 ttcagatcga aaaaggaacg ggtggctggg gcggcccgct tgagctgaaa gccacgccgg
    21841 gaaaaaaaat cgtctatatc accgccggta cccggcctgc gattgttgac aaactggcac
    21901 agcttactgg ctggcaggct attgacggat ttaaagaagg tgaacccgcg gaggcggaaa
    21961 ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg cggcatctat ccgaaacgac
    22021 gtattcccac cattaatatc cactcgacgg gcaagtccgg tccgctggcg cagtacattg
    22081 tggaagatat ttatgtctct ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga
    22141 caccacaacc ctcttccgtg ggccgtgact atgacaccag taagaaaatc accgaacaaa
    22201 gcgatggttt actggcgaag gtgggaatgg gcatggggtc cgccgttgcg gtgctgtttc
    22261 aatctggtcg tgacaccatc gacactgtat taaaaaccat tctgccgttt atggcattcg
    22321 tctcggcgct cattggcatc attatggctt ctggccttgg tgactggatt gcccacggtc
    22381 ttgctccgct ggcgagccat ccactgggtc tggtcatgct ggcgctcatc tgctccttcc
    22441 cactgctttc acctttcctc ggcccaggcg cagttatcgc acaggttatc ggcgtattga
    22501 ttggcgtgca gattggtctc ggcaatattc cgccgcatct ggctttaccg gcactgtttg
    22561 ccatcaacgc gcaggcggcc tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc
    22621 gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg
    22681 cgccgactgt actgatcgcc tggtttgtct ccggttttat ctatcaatag aggctgaaac
    22741 atgaccgtta tttatcagac caccatcacc cgtatcggcg cgagtgccac tgacgccctc
    22801 agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag cggacctcga agagtattgc
    22861 ttcattcatt gccacggcga gttgaaaggt gaactccatc ctggtttgca atttacgctc
    22921 gggcagcatc gctatccggt gaccgccgtt ggcagcgtgg cggaagacaa ccttcgcgaa
    22981 ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag cggaatttcc gggcactgtc
    23041 catgtggcag gccctgtacc cgacgatatc gcgccgggat cggttttgaa gtttgaatct
    23101 gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc
    23161 gcgttcctgt gccacggtct ggctgccgag gggtatcgcg tcgcggttgt cgatattcag
    23221 agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg aatatggtga aagtatggcg
    23281 tacggttttg gtgctgacgc cactagcgag caaagcgttc tggcgctctc tcgtggggta
    23341 gatgaaatct ttggtcgcgt ggatttgctg gtctatagcg ccggaatagc aaaagcagcc
    23401 tttatcagcg acttccagct cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt
    23461 tatttcctgt gtgcccgtga attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc
    23521 attattcaga tcaactcgaa atccggcaaa gtgggcagca aacacaactc tggctacagc
    23581 gcagcgaaat ttggtggcgt cgggctgact caatcactgg cgctggatct ggcggagtac
    23641 ggcattacgg tgcattcact gatgctcggt aacctgctga aatcgccgat gttccagtca
    23701 ctgttgccac aatacgcgac caagctgggt atcaaaccgg atcaagtcga gcagtattac
    23761 atcgacaaag taccgctcaa acgcggctgc gattatcaag atgtgctgaa tatgctgctg
    23821 ttctacgcca gtcctaaggc gtcgtactgc accggacagt cgatcaatgt caccggcggt
    23881 caggtgatgt tctgatcaac agcggagatc cattaaggat ctccgtgaga ctatagaatg
    23941 cctgatgcgc tacgctcatc aggcatacag gacttccgcc actacattaa ggaaaagtta
    24001 tggtatccgc actcatcacc gtcgccgtta tcgcctggtg tgcgcaactg gccttaggcg
    24061 gctggcaaat ttctcgtttt aaccgtgcct tcgacacact atgccagcaa gggcgggttg
    24121 gcgtgggccg ttccagcggg cgctttaaac cacgggtcgt ggtcgccatc gcgctggacg
    24181 atcagcagcg catcgtcgac accttgttta tgaaaggact gaccgtcttc gcccgaccgc
    24241 aaaaaattcc cgcaattacc ggtatgcatg cgggtgattt acagcccgat gtgatctttc
    24301 cccatgatcc actatcacag aatgctctat cattggcgct taaactgaaa cgtggataat
    24361 ttcgttgtga atgttacttg cttgcgaagt tatcattttg aaacctaaat caggtaatca
    24421 cgcccatgaa acctcgtcag cgtcaggccg ccattctgga gtatctgcaa aagcagggta
    24481 aatgctcggt tgaagaattg gcgcaatact ttgacaccac aggcacaacc attcgcaaag
    24541 atctggtcat tctggaacat gccggaaccg tcattcgtac ttatggcgga gtggtgttga
    24601 ataaagagga atccgatccg cctatcgatc ataaaacact catcaacacc cacaagaaag
    24661 agctgattgc agaagctgcc gttagtttta tccatgatgg cgattcgatc attcttgatg
    24721 ctggcagtac cgttttgcag atggttcccc tgctctcgcg ctttaataac atcacggtga
    24781 tgaccaacag cctgcatatc gtcaatgcgc tatccgaact ggataacgaa caaactatcc
    24841 tgatgccagg cggaacgttt cgtaaaaaat cggcctcatt tcacggacag ctggcagaga
    24901 atgccttcga acatttcagt ttcgataaat tgtttatggg caccgacggc atcgatctca
    24961 atgcgggcgt aaccaccttc aacgaggttt ataccgtcag taaggcaatg tgcaatgccg
    25021 cgcgcgaagt gattttgatg gcggactcat caaagtttgg ccgtaaaagc cccaacgtag
    25081 tttgcagtct tgaaagcgtc gataagctga ttaccgacgc aggtatcgat ccggcgtttc
    25141 gtcaggcgct ggaagagaaa gggatcgatg tgatcataac cggagagagc aatgagtgaa
    25201 gcactactga acgcgggacg tcagacgtta atgctggagt tgcaggaagc aagccgttta
    25261 ccggaacgtc tgggcgatga ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc
    25321 aaagtggtgg tttcgggaat tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca
    25381 cttgccagta ccggcacacc ggcttttttt gtccatccgg cagaagcact gcacggcgat
    25441 ctggggatga ttgaaagccg cgatgtgatg ctgtttatct cttactccgg tggcgcgaag
    25501 gaactggatc tgattattcc gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc
    25561 ggcaaaccga cgtcaccgct gggcctggcg gcaaaagcgg tgctggatat ctccgtagaa
    25621 cgcgaagcct gcccgatgca ccttgcgccg acctccagca ccgtcaatac cctgatgatg
    25681 ggtgacgcgc tggcgatggc ggtcatgcag acgcgcggat ttaatgaaga agattttgcc
    25741 cgctcccacc cagccggggc actgggcgct cgcttgctga ataaagtgca tcatctgatg
    25801 cgccgtgacg atgccatccc acaggtggcg ttaaccgcca gcgtgatgga tgcgatgctg
    25861 gaactcagcc gcaccggtct ggggctggtg gcggtatgtg acgctcaaca acaggtacaa
    25921 ggcgtcttta ccgacggcga tttacgtcgc tggctggttg gcggcggcgc actcaccacg
    25981 ccagttaatg aagcgatgac gacaggcggc accacattac aggcacaaag tcgcgccatc
    26041 gacgccaaag agatcctgat gaagcgcaaa atcactgccg caccggtggt ggatgaaaac
    26101 ggcaaactca ccggcgcaat taacctgcag gatttctatc aggccgggat tatttaatcc
    26161 ttcaatccca gacgtttcgc cagccgatgc aggttggcga cgtcggtttc cagcatccgc
    26221 gcgcaggcag cccagttgtg atgattttgt gccagtgcct ggcgaatggt ttcacgctgg
    26281 aacgcttctg tcgcttcacg caggttttgc ttaacaacgg gcaccgccgc cgcttctggc
    26341 ggcggcaacg tcacctcagg aaaagcaaaa tgttgtgcct caagaatcac ttcatcgccg
    26401 ctgcgagtgg ctctcgccag cactaccgcc cgatgaatag catgttccag ttcgcgcacg
    26461 ttccccggaa aacgatagtg ttgcagtaaa tttcgcgctc cggcacttaa taccacgcgg
    26521 gagagcccca gccgcaaacg acactgctcg cagaaatacc ccgccagcag aatgacatca
    26581 tcgccccgct cacgcagcgg cggcaccgaa agtggaaaca cgctcaggcg atgaaacaaa
    26641 tcggcgcgga atcgccctgc cagcacctct tcgcgtaagt cgcggttagt cgccgccagc
    26701 acgcgcacat cgacccgcaa actacggtca tcgccaacgc gctgaatatc gccatactgc
    26761 aacaccctca gcagcttggc ctgcaatgcc aacgacaact cgccgatctc atcgagaaac
    26821 agagtgccgt tatccgccat ttcaaacttc ccgctgcggt tactgatagc gccagtaaac
    26881 gctcctttca catgcccgaa caactcactt tccgccacac tttccggcag tgcggcacag
    26941 ttgagataga ccagcggatt caccgcccgt ggcgaggctt catgaatcgc tttcgccacc
    27001 agctccttac cggttcccgt ctcaccgctg atcagaacgt tgagatcgga cgccgccaca
    27061 atctcaatct cttttttcaa ttgcgtcatg cctggcgaca agccaatcat ctgcgtctgt
    27121 ttcaccgctt caaacggcgc ggcatcgccc ggcagcatat tctggctttc cagttgttca
    27181 atcagcaacg cattgcttaa cgctcccgcc gccagcgcgg caatcagccg taactcttcg
    27241 tcgctgaaaa catcgaactg atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg
    27301 ttttgcccgg caaacaatgg cagaccaacg caggcgtgaa ccttcagact ctcctgccca
    27361 ggaatcaaac cgtcataggg atcgggcaat tcgctgtctg caggaaagcg caccacgtcc
    27421 ccggcgcggg caatcgcttc aagccgtgga tgcccttcca gcgcaaagcg tctaccgagt
    27481 acatcctttg ccagaccgtc gatggcaagc ggaataaact gccgcgaatc gtaacgtagc
    27541 aacgcagacg catcgcactc cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga
    27601 tcctgatgac caatcccacg ctgcaattcg atggcgatat tcgccagcac atcaacggaa
    27661 aaactcatct ttgcctcact gtcaatttga ctatagatat tgtcatatcg accatttgat
    27721 tgatagtcat tttgactact cattaatgag cataatttta tttatagagt aaaaacaatc
    27781 agataaaaaa ctggcacgta atctgcaatt agcaagacat ctttttagaa cacgctgaat
    27841 aaattgaggt tgctatgtct attgtggtga aaaataacat tcattgggtt ggtcaacgtg
    27901 actgggaagt gcgtgatttt cacggtacgg aatataaaac gctgcgcggc agcagttaca
    27961 acagctacct catccgcgaa gaaaaaaacg tgctgatcga caccgtcgac cataaattca
    28021 gccgcgaatt tgtgcagaac ctgcgtaatg aaatcgatct ggcggatatc gattacatcg
    28081 tgattaacca tgcagaagag gaccacgctg gggcgctgac cgaactgatg gcgcaaattc
    28141 ccgatacgcc gatttactgt actgccaacg ctatcgactc gataaatggt catcaccatc
    28201 atccggagtg gaattttaat gtggtgaaaa ctggtgacac gctggatatc ggcaacggca
    28261 aacagctcat ttttgtcgaa acgccaatgc tgcactggcc ggacagcatg atgacttacc
    28321 tgacaggcga cgcggtgctg ttcagtaacg atgctttcgg tcagcactac tgcgacgagc
    28381 atctgttcaa cgatgaagtg gatcagacgg agcttttcga gcagtgccag cgttactacg
    28441 ccaatatcct gacgccgttc agccgcctgg taacaccgaa aattaccgag atcctgggct
    28501 ttaacttacc agtcgatatg atagcgacct ctcacggcgt ggtatggcgc gataacccga
    28561 cgcaaattgt cgagctgtac ctgaagtggg ccgctgatta tcaggaagac agaatcacca
    28621 ttttctacga caccatgtcg aataacaccc gcatgatggc tgacgctatc gcccagggaa
    28681 ttgcggaaac cgacccacgc gtggcggtga aaattttcaa cgtcgcccga agcgataaaa
    28741 acgaaatcct gacaaatgtc ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga
    28801 ataacgtgat gatgccgaaa atcgccgggc tggtggagga gatgactggt ttacgcttcc
    28861 gtaacaaacg cgccagtgct ttcggctctc acggctggag cggcggtgcg gtggatcgtc
    28921 tttccacgcg cctgcaggat gcgggtttcg aaatgtcgct tagcctgaaa gccaaatggc
    28981 gaccagacca ggacgctctg gagttatgtc gtgaacacgg tcgcgaaatc gcccgtcagt
    29041 gggcgctcgc gccgctgccg cagagcacgg tgaatacggt ggttaaagaa gaaacctctg
    29101 ccaccacgac ggctgacctc ggcccacgga tgcagtgcag cgtctgccag tggatttacg
    29161 atccggcaaa aggcgagcca atgcaggacg ttgcgccagg aacgccgtgg agtgaagtcc
    29221 cggataactt cctctgcccg gaatgctccc tcggcaaaga cgtctttgaa gaactggcat
    29281 cggaggcaaa atgagtaacg gcattgtgat catcggttcg ggcttcgccg cccgccaact
    29341 ggtgaaaaat attcgcaaac aggacgccac tattccatta accctgattg ccgccgacag
    29401 catggatgag tacaacaaac ctgacctcag ccatgttatc agtcaggggc aacgtgccga
    29461 tgaccttacc cgccagacgg cgggtgaatt tgccgagcag tttaatctgc acctgtttcc
    29521 acaaacctgg gtgacggata tcgatgccga agcccgtgtg gtgaaaagcc agaataatca
    29581 gtggcaatac gacaagctag tactggcaac cggtgccagt gcctttgtcc cgcctgtgcc
    29641 tgggcgtgag ttaatgctga cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca
    29701 actgcgggat gcccgacgcg tgttgattgt tggcggtggt ttgattggta gcgaactggc
    29761 gatggatttt tgtcgtgcag gcaaagcggt cacgctaatc gacaacgctg ccagtattct
    29821 ggcgtcatta atgccaccgg aagtaagcag ccgcttgcag catcggttga cggagatggg
    29881 cgttcatctg ttgttgaaat ctcagttgca ggggctggaa aaaacagatt ctggcattct
    29941 ggcaacgctg gaccgccagc gcagtatcga agtggatgcg gtaattgccg ccaccggact
    30001 gcgcccggaa accgccctgg cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt
    30061 cgatagttat ctgcaaacca gtaatgccga tatttacgcg ctgggcgatt gcgcggaaat
    30121 taacggtcag gtattgccgt tcctccagcc gattcaactt agcgcgatgg tgctggcaaa
    30181 aaatcttctc ggcaataaca cgccactgaa actaccggcg atgctggtga aaatcaaaac
    30241 gccggaatta ccgctgcatc tggcaggcga aacccagcgt caggatttac gctggcaaat
    30301 taataccgaa cgccagggaa tggtggcgcg cggcgttgac gatgctgacc agcttcgcgc
    30361 ctttgtggtc agtgaggatc ggatgaaaga ggcatttgga ttgttgaaaa cattgccgat
    30421 gtaggtgggc tactgtgcct aaaatgtcgg atgcgacgct ggcgcgtctt atccaaccta
    30481 cggggagcgc atgtgttggt cacatactgc gttcatgtcg catccaccta ctgtgcccaa
    30541 aatgtcggat gcgacgctgg cgcgtcttat ccgacctacg gggacgcatg tgtaggccgg
    30601 ataaggcgtt tacgccgcat ccggcaatgg tgtccaaatg caacacgttt tatccgttct
    30661 ggacttcacc cgctaaccaa cgcgccgcag caataacccc ctgccccaga gacaaaccgc
    30721 catcacccgc cggtaaactc tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca
    30781 gacgtgcacg cagcaaacgg ttatgaataa ccccgccgct aaataccagc gtagtgatac
    30841 cacgcatcgt ggcctgctca cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa
    30901 acgcccacgc gcgttgatta accggtgcct gccagttcag ccactgctgc cagaaagtgg
    30961 cgagatccag ttgattgtcc accagcggca ttgtcaccgg atgcgtcact ccgtggcacg
    31021 aagctgcgag tgcctccaga gcacaagccg cttcaccttc ataacttaac gtggctggcg
    31081 cacagcccag tgccgccgcc acggcatcga acaaccgccc acacgatgac gccagcggcg
    31141 cgttaattcc acgctcaatg gcccgcgcca gcacgctcca gttttgctgt tgcacacttg
    31201 ctgtttcaga ataattctgc cactccggca caaagcgcag gcactgcgcc agcaggtttc
    31261 gccacggctg cgtcgccgcc aaatcgccac ccggaagcgc cactgcaggc aagccgccca
    31321 ggtgctcaca ttcgcgatag ttcacccgca gacactcgcc gccccacaaa gcgccgttct
    31381 cccccatacc gataccatcg agcgtcaaag caatgacatc accgccatcc agcggccact
    31441 gatgctctgc cagacacgcc gctgcgtggg catgatgatg cagcaccgtt tgcgtcggca
    31501 tattcatttc gctcgcccac tggctggaga catagcccgg atgcacgtca tgcacaacgt
    31561 attgcggagt gaaatcgtag atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct
    31621 ggatgccatc gtcacttaaa tcgcccagat gctgactcaa caccgcttgt tcaccgcgca
    31681 ccaggcagaa ggtgtttttc agatccgcgc caagacacag cacaggcgga acatttttaa
    31741 agcccggagg caaaaccagc gcatccggca catacccgcg tgaacggcgc agcatttcgc
    31801 cgctttcgcg caccaccgaa tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga
    31861 atccgtcggc aatgccctgc aaatccgcca gcgcctgttc gttgctgata gctggtggtt
    31921 taccgctcag gttgccggag gtcatcacca gcgggcattg cagttcctgt aacagcaaat
    31981 gctggagcgg gttcgcaggc aacattaccc cgacttcgtt aaggtcaggg gcgatatcat
    32041 cacaaagctc aggaacgtat tttttatcca ccagcacaat cggcgcggcg ggcgtggtaa
    32101 gcaactggcg cgcagcgtct ggtaaaccgt cagccactgg caacatgacc gccagcggtt
    32161 tcgccgggcg atgtttgcgc gcccgcagtg tcgccaccgc gttactgtta cgtgcatcgc
    32221 aggcaagatg aaatccgcca atccctttga tggcgacaat gttgcccatt tttaactgtg
    32281 cgatagccgc ctgtaatgcc gcctcttgtt ctgcatgttc accatgactt acccattcaa
    32341 gatgtgggcc acactccggg caggccaccg gctgggcgtg gaagcgacga tcgagcgggt
    32401 cgcggtactc tttgtcacaa gccggacaca acggaaacgc cgccatcacg gtaaacgggc
    32461 ggtcgtaagg catggcgcga ataatggtga aacgcgggcc gcagtgggta cagttgataa
    32521 acggataacg ataacgccgt tcgcctgggg tattcatttc ggcaagacag gcagggcaag
    32581 tagcggcatc cgggacaatt tgcgtattca tggcacctcc cgcgctctgg cgtatagtga
    32641 actcggtggg cagttgtgac cagatgtacg gctcacgctc gacgctatca atacgcgcca
    32701 gcggcgggca gtgctgatgc aattgaacaa gaaacgtttc cgggtcttcc agcagccgga
    32761 cttctacgcc atcgccgtca ttacagacat cgccgtgaag atttaattgc tgtgccagct
    32821 gccagacaaa cggacgaaaa ccgacgccct gcactttgcc acgaatacgc agttggacac
    32881 cgcaagatgt gttttttgcc attgagttat tcccgccatc atgaattgcg taacccgccc
    32941 tgccggacac gactgcgtcg tatccggcag tctcaggtcg gcgataccgc cagctccgta
    33001 ttctacgaat atttccggga attcctttga tgccagaaca gttctataag atttttagaa
    33061 catcagcgcc gtacggcgac gtttttctgc gctcagttgt tcaagtttat tacgatcgac
    33121 acaaatcagc gcatgggtcg ggcaagccgc catacacgcc gggccgtctt cgcgatggtt
    33181 gcacaggtcg catttattgg cttcagcttt gtcagcccgt acattcagcc ccgcgccgct
    33241 gttgcggatc accggacgta ccaccacttc catcgcgcca tacgggcaag ccaccacgca
    33301 ggttttgcaa ccaatgcaac gttcctgcat cacatgaaca aaccctttat cacggctgat
    33361 agcaccattc gggcagacgt tagcgcacgg tgcatcttca cactgacggc agactgtcgc
    33421 cgtggaaatg ttcacacctt taatgacatg gattcgcggt aaaaaggttt ccggggtcag
    33481 cgatgcacag tcctgatttt cctgatgaga aaccacgcac gccacttcac aggtacggca
    33541 accaatacat ttactcgcgt cagcaatgat gaaacggttc atcaaattct ccagcaatga
    33601 cagttaatgc gccgatacat tcacaaatca tgccagtttt taattcactg ttatttaagg
    33661 aaattaattt ctgtaatgca ggaaaaaaga tgtcatcgac actagtgacg atgacatgtg
    33721 atgacaatgt ttatcgcgaa agagcaatga gtgagtcgcg gcggatcagt tttccgctga
    33781 aggttttcgg cggtgagaaa tccccgccat cgagcataaa aatcagccgt ccaataattt
    33841 cctgaatcat ctcagtcacc ggaattttta cgctggagag cgccggaacg atataggggg
    33901 caattgcgat atcatcgaat ccgataactg acacctgctc tggcaccgct acgccgcgct
    33961 cgtgcaacgc tttcatcgca cctatcgcca tatcgtcgtt actggcaact aacgcgctaa
    34021 atttagcccc acgttcgagc aacgtttcta ccccttcggc cccgctggcg ggcgtccatt
    34081 taccgttagc gataagtttt tcattgagcg caataccatg ctgcgccagc gcgtctttat
    34141 acccagcaag acgttcaatg ctggtggggg aatccatcga gccggtaagg aaagcaatct
    34201 cctgatgtcc ggcgtttatc aactctgcca cggcattaaa gctggtctgt ttatgatcgc
    34261 accagacgct atggctgcta tttttgcgca ggcggcgatt aagcaccatt atcggctgac
    34321 tgtgcgcgtc aataatgtca tcgatctcat ccacgcttaa aaaacgcggg taaatcataa
    34381 tggcgtcgca gcgcaaatcc agcagatact gaatcgcctg gcgctcttct tcggcgctgt
    34441 gtttaccatc tgccaatagc aactgccgcc ctttctcttc cgccattcgc gcggcatgaa
    34501 agagtaattc actaaaataa atgccgtggt aaagcgtgtt ggtcactacc agccccagcg
    34561 tctgagtact cttcgccgac agattgcgcg ccagcaagtt tgggcggtaa ccgctctctt
    34621 ctaccgcctg aaacacgcga tctttagtct cctggctgac gtagccatta cctgaaagca
    34681 cgcgggaaac ggtcgctttt gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca
    34741 tcattttcat ccctttacac gcaatcaacg cagtgtactg caccgtttgc cgattgtcct
    34801 tgcgcaatcg gcgggaaaaa tattcaggtg accggtttca caaatataaa aaatgaacaa
    34861 ttaaccctct tgcttattta gtgacaacta ttcatgattt tgtgaaaccg gtttcctaat
    34921 tccgtttcag catcggcatt tttccgtcac gtcgactgat aacaactaca tctaccctac
    34981 tgataacagg ataaaatccg atggccaaaa attatgcggc gctggcacgc tcggtgatag
    35041 cggcactggg cggcgttgat aacatctcgg cagtcacgca ctgtatgacg cgtttgcgct
    35101 ttgttatcaa agatgaccag cttatcgaca gcccgacgtt aaaaaccatc cccggcgtgc
    35161 tcggcgtggt acgtagtgac aaccagtgtc aggtgattat cggcaatacc gtttcacaag
    35221 cctttcagga agtcgtcagc ctgctgccgg gagatatgca gcccgcacag cccgtaggta
    35281 aacccaaact cacgctacgt cgcattggtg cggggatcct cgatgcgctg atcggcacca
    35341 tgtcaccgct gatcccggcg attatcggcg gatcgatggt caaactgctg gcgatgatcc
    35401 tcgagatgag cggcgtgctg acaaaaggat cgccgacctt aactattctg aatgtgattg
    35461 gtgacggtgc tttcttcttc ctgccgctga tggtcgcggc ttcggccgcc atcaaattta
    35521 aaaccaatat gtcgctggcg attgccatcg cgggtgtgct ggtacatccg agctttattg
    35581 aactgatggc gaaagcggcc cagggtgaac atgtcgaatt tgccctgatc ccggtcaccg
    35641 cggtgaaata cacctacaca gtgatcccgg cgctggtgat gacctggtgc ctgtcatata
    35701 tcgaacgctg ggtggatcgc attacaccgg cggtgaccaa aaacttcctc aaaccaatgc
    35761 tgattgtgtt gattgccgca ccgctggcaa tcctgctgat tggcccgatt ggtatctgga
    35821 tcggtagcgc catttcggcg ctggtttaca ccattcatgg ttatctgggc tggctttcag
    35881 tcgccattat gggcgcgctg tggcctctgc tggtaatgac cgggatgcac cgcgtcttta
    35941 cgccaaccat cattcagacc attgccgaaa ccggcaaaga agggatggtc atgccgtcag
    36001 agatcggcgc taacctgtcg ctgggcggtt catcactggc ggtggcgtgg aaaacgaaaa
    36061 acccggaact gcgccagacg gcgctggctg cggcggcatc agccattatg gcggggattt
    36121 ccgaaccggc gttatacggc gtggcgatcc gcctgaaacg tccgcttatc gccagtctta
    36181 tcagcggttt tatttgcggt gcggttgccg gtatggcggg gcttgccagc cactcaatgg
    36241 cagcgccggg gctatttacc agcgtgcagt tcttcgatcc ggcgaatcca atgagcatcg
    36301 tctgggtgtt cgcggtcatg gcgctggcgg tcgtgctgtc gtttatcctc acactgttgc
    36361 tcggctttga ggatattcct gttgaggaag cggctgccga ggcgcgaaag catcagagcg
    36421 tacaaccgac cgtcgccaaa gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc
    36481 agaaggtttt ttatggggcg gcgcgcttgc cgccaaccag tctgaaggtg cgttccgtga
    36541 aggtggcaaa ggactgacaa cggtcgatat gatcccacac ggcgagcatc gaatggcggt
    36601 gaaactgggg ctggaaaaac gttttcagtt gcgcgatgac gagttttatc ccagccatga
    36661 ggcgacggat ttttatcatc gttataaaga agatatcgcc ctgatggcag agatgggatt
    36721 caaagtgttc cgtacctcaa ttgcctggag ccgcctcttc ccgcagggcg atgaactgac
    36781 gcccaaccag cagggcattg ctttttaccg ttctgtcttt gaagagtgta aaaagtatgg
    36841 tatcgaaccg ctggtcacgt tgtgccactt cgatgtgccg atgcatctgg tcaccgaata
    36901 tggctcctgg cgtaaccgca agctggtgga gcttttcagc cgctacgccc ggacctgctt
    36961 tgaagcattt gatggtctgg tgaaatactg gctaaccttc aatgaaatca acattatgtt
    37021 gcatagcccg ttctccggcg cgggtctggt gtttgaagaa ggtgaaaatc aggatcaggt
    37081 gaaatatcag gccgcgcatc accagctggt tgccagtgcg ctagccacca aaatcgccca
    37141 tgaggttaac ccgcaaaatc aggtgggctg tatgctggcg ggcggtaact tctaccctta
    37201 cagttgcaag ccggaagatg tctgggcggc gctggagaaa gaccgggaaa acctgttttt
    37261 tatcgatgtg caggcgcggg gcgcgtatcc ggcttactct gcccgcgtat tccgcgaaaa
    37321 aggggtaacc atcgacaaag caccgggcga tgatgaaatc ctgaaaaaca ccgtcgattt
    37381 tgtctctttc agctattacg cctcgcgctg cgcctcggcg gagatgaacg ccaacaatag
    37441 cagtgcggcg aacgtagtga aatcgctgcg taacccgtat ctgcaagtga gcgactgggg
    37501 ctggggtatt gatccactcg gtctgcgtat caccatgaac atgatgtacg accgttatca
    37561 gaagccgctg tttctggtgg aaaacggcct cggcgcaaaa gatgaacttg ctgccaatgg
    37621 cgagattaac gatgactatc gcatcagcta tttacgcgaa catatccgcg caatgggcga
    37681 agcgattgca gatggcattc cgctgatggg ctacaccaca tggggctgta ttgatttagt
    37741 ttccgcctct acgggtgaaa tgagcaaacg ctacggtttt gtctacgtag accgtgacga
    37801 cgcaggcaac ggcacgctga cgcgcacgcg taagaaatcg ttctggtggt ataaaaaagt
    37861 gattgccagt aatggggaag atttagagta agtaaccgtg ccggatgcgg cgtgaacgcc
    37921 ttatccggcc tacatagttc gtgcgacatg taggcccgat aagcgcagcg catcgggcaa
    37981 tctggggttt ggcatcagtc aaaatcaaag aaaccattcc ggcttaacgc caaatcgggc
    38041 tgaaagtgcc ttaatgtggg atattgtcag ggatcgttgt ccagacaaaa tctgactgac
    38101 cagggattta gagccgattt cgttcttcaa atccgcatag gtcagattat gttgatcaat
    38161 caacgtgcga agtaacgcca cgccaaccgg cattgccgca acggctttgt cgaactctgc
    38221 aaacttttcg ttgttgtttt catactctgc aatgcggctc gccagaaagt cgataagtgg
    38281 attggtgtca tcttcttcaa tcaaatattc gaccagttcc agtgcttcac gataatcatc
    38341 ttctgagtca ctgcctccca gaaaggggac tgcattgacc agttctcttg ttgctttaac
    38401 agcgcgtgca gcattagcag tcattctttg ttctctctgt agtaacgtga cgtctgtctg
    38461 cacgtaatcc ccctcatagc cactctgcaa aaagtcagtt ctggttcacc ccataaagca
    38521 catccccctt tatcaagcaa cgctgcgtct gcgccagatc ttccgtttgc gggtcgcgcc
    38581 cggcgatggg tttgaggacg agtttacggt tgggggtata agtaaaacgc gtgagcgcgg
    38641 gatagcttaa cttgccaaac cagccgtgaa cacgctgcca tttggcgtct tttacctgat
    38701 acagactggt tatccagtaa ccggaatcaa actgcatatc cagcaattgc gtgtggccat
    38761 tcctttgtaa atccagcaaa tcatcaatgc cagtttcact tgcggtataa aaaccatcgg
    38821 actgaaaaac acacgggcga ccttgctgat taaaggtcat cagaatcaaa aacgcgttag
    38881 gtgccagacc atttcctgaa ataccccgcc agataaccag atcctgaatg ccgttacgat
    38941 caagatcggc acggtaaagt tgcgcattac ccgccatatt ttgcagcacc cccacaggca
    39001 cagaccagga tttaccggcg cgatctttac cgttcaattg ccattcacta tcatcatttg
    39061 gcatcttttc aagcgtcacg ccagcaacgc cgacttttct aaaatgccag ttttgctcgg
    39121 catcataagg catggtcagg agaggtttca gcgcccgctt ttgcgcgtat tcgctgtcac
    39181 tcatcggcag atattcggta aagctggtgg cctgtgcagg cagcactaag ctcaataaaa
    39241 aagcgcacat ccctgttctg atgttcaatt ccctgaattc cttaatgaaa aactactcta
    39301 cttccaccgc taactgcgca aatccaccat ttccttccca accttccagt cgttgataaa
    39361 cggtttcaac cgcatcttta atcggctggg tcatcgggta gtaaaagccg acgatatccg
    39421 gctggatgcc gaggaaaatc acttcgccaa tatcttcttt caactggtcg ataaggtagt
    39481 tcaacggcat gttatgggta gtcatcataa acatctcggc gatatcatcc gggtcgatga
    39541 tgcggatctc gccggggttt agccccatat cagtggcgtc gacaatcagc agtcgcgtag
    39601 ggcgcagttc acggatggcg acgatgtcgt tttccggtgc actaccgccg tcaatcacca
    39661 cccagttacc tttcggcgcg gcggcgcact tttccgccag cagcgggcct gcgccatcat
    39721 cgcccatcat gctattgcca acacagagta aaacgtcagt cacgcagtct cctcaccatc
    39781 aaatagatgg cgttttcctg atgaatatca tgcagcatac ttagcagcat tgtactccat
    39841 tcctgctgct gcgaagtttg cgctgcccgg gctttatcga atgcgttggc cagcatcggc
    39901 acatggttaa tgtcgatgac gatctcacca tatttcggca cgccttccat tttgcggcgg
    39961 gcttcgctgc ccgcctccag agtggcaatc catgccagat attcatccca cgggcaggtg
    40021 agcgccgctt ccaggcaatc gataacccca aggtggtgac caatcgccag gctgtaatag
    40081 accacctgct gcgcctcggc gggcgtggca tcgttctcat caataaattt acggctcagt
    40141 tgactgaaca ccaccttttc actcatcgga tacgcgcctc ttctacaaca tgattcagat
    40201 ggctgacaat ctcgttcaga cgcggatcgt tttccgcttc cagccagcgt gcaacctgtt
    40261 cttcgccctg ccctaactgt gtcaggaagt catcggcaat ctgacgaccg taacgataac
    40321 ctgccaggcg gcgtgcttcg cgatccactt tcacacgcag cggctgcacc atatcgccat
    40381 gcaggatttc cgccggttgt tcatcctgct cacccgggcc acgggcgtga attttctgct
    40441 ccagcaggcc gagcgccatt gcaaagccat acagcgtggc ggcaggcgtt ggcgggcaac
    40501 cagggatgta gacatctacc gggacgattt tatccgtacc gccccacacg cagtagagat
    40561 cgtggaagat cccgccactg ttaccgcagg caccgtagga gatacagatt ttcgggtccg
    40621 gcgcggactg ccacgcacgc agcgcagggg atcgcattgc acgggtgacc gcgccggtaa
    40681 acagtaaaat atccgcatga cgcggtgaag gaacgacttt aatgccgaag cgttctgcat
    40741 caaacagcgg cgaaagcgtg gcgaaaattt cgatttcgca gccgttgcag ccgccgcagt
    40801 ccacgcggta aacgtaggca gaacgtttga tttttttcag taacgacgcc ttcatgctgg
    40861 cgatggattc atccaccgtc atcgggaccg gaatgccgtt ggcgtcacgg gggcctaata
    40921 aattgctcat cagatggcct ctttcatatg gcgagtcagt tcaatacggt cggacggcac
    40981 caggcatttc tggcgcttac attccgggca agtctcaaag ctttcgcggt ggttttccgc
    41041 gcggctgtca ccattgtgct taagcagcgc aatggcgtag tcgatctctt tctggacggc
    41101 gaaaggacga ttgcagacgc gacagctgca cagcacaaaa cgggactgtt gcaggaaatc
    41161 ttctttcttc cacaccgcca gttcgtactc ttgcgacagt ttgatcgccg ccgtcgggca
    41221 aacttcttcg cagcgtccac agaagatgca gcgcccgaga ttaaactccc acgcaagctc
    41281 tcctgtggcg aggtcggttt caaccgttaa agcgtttgac gggcaggcat tgacgcaggc
    41341 cgcgcagccg atgcactgct gcgggttctg ctctggctta ccacggaagt ttttatcaac
    41401 cgcaatcggc tccagcggat aagacgaggt cgccgtgccg gttttgatga cttttttgat
    41461 aaaggtaaac atggcgattc cttatttcag cggcgagttt ttacgctcaa tgctgtaacg
    41521 ctcgagttct ttgtacggca ccactttgct cttcttctta cgtacatcga ccaccgtcat
    41581 gcggtcggta caggagtagc aagggtccag gctaccgata atcagcggcg catcagaaac
    41641 ggtgttgcca cgcagcatgt agcgcagggt cggccagttc gcgtaggtcg cggcacggca
    41701 gcgccagcgg tacagcttct ggttgtcgcc ggtcatgctc cagtggatat cgtcgccgcg
    41761 cggcgcttcg gcaaagccca gcgcgaaacg gtgcggaatg taggtaaagc cttccaccat
    41821 caacggaccg cccggcaggt tatccagacc gtagtcgatc atgttcagcg cggtatagac
    41881 ctcgttgatt cgcactttca gacgggaaat aacgtcgcag ccctgctcgc tgtggacttc
    41941 cattggcagc aggccataac caacaaacgg gtgatcggcg cgggtatcgc gagcgtgacc
    42001 gctggcacgg accatcgggc caacgttact gaagtcgcgg gcaatttccg ggtcaagacg
    42061 accaatgcca acagtgcgct gttccatgtt cggtgtgctc aacagcacat ccaccagctc
    42121 ctgcacttca cgacgcatct gttgcgccag ctggcgggtc tggatcatgt catctttcag
    42181 cagatcgcga cgaatcccgc cgatcaggtt caggccgtag gttttacgcg caccggtaag
    42241 gatctccgcc attttcatgg aggtttcacg cacgcggaag aactgcataa agccggagtc
    42301 gaagccggta aagtgacagg cgaggccgag gttgagcaga tgcgagtgca ggcgttccac
    42361 ctccagcaga atggcgcgga tcatctgcgc acgttctggc acctgaatac ccatcgcgtt
    42421 ttccaccgac gtggtgtagg cggtgctgtg agcaaagccg cagatgccgc acacacggtc
    42481 agagaggaag gtcacttcgt tgtaacccat acggatttcc gccagttttt ccatgccgcg
    42541 atggacgtag aacaggcggt agtcggcgtc gataatgttt tcgccatcga cgaacagacg
    42601 gaagtgaccc ggttcgtcag aagtgacatg cagcggacca atcggcacga cgttgttttt
    42661 cttgtcgccc agttcgttga tgaactcgta ggtttcagca tcggtggtcg gtgccggacg
    42721 ctgacgataa tccatgctgt cttttcgcag cggataaagt tcatccggcc agtcatccgg
    42781 cagcaccaga cgacgttcat ccggcagacc aaccggaatc aaaccgtaca tatcgcgcac
    42841 ttcgcgctcg ccccacaccg ccgccggaac gcgcggcgtc acggacggat attccggttt
    42901 gttggcgtca acttcgacgc gaaccgttac ccaacactta gtgcccttct ccatcgacag
    42961 cacgtaataa acggcgtaat gaccattcag tttgcgttcg tcgttaccaa acagcaccga
    43021 cagccagcca ccctgcttgt agtagagaaa ctccaccact tccggcagat agttcacctt
    43081 tacggtgaca gtcagctgat ctttggtctg ccaggcgtgg tccagcacga cgcccggaaa
    43141 tgcctcattc agcgcggcga gataatgttg acctaatttt tcttcagaca tgctcaaact
    43201 ctctttaatc acgccgccag caaggagacg aacgctaaaa atgcaaagcc aaacccagcc
    43261 caggtaatgc gcggagtaat atcaagacgc agacgggcca tgctgttttc gaacagcgcg
    43321 ataaccagga cgccgaccac cagtttgacg atggcaatca ccagcgccag cagcagccca
    43381 ccgacggtga aggtttccat ttgtccccac ggaataaaca ccccgacgaa catctgcagc
    43441 accaccagct gtttcaggct gataccccat ttcatgacgc caaagccgct gccgctgtac
    43501 tcagagagcg gaccttcctg caactcctgc tcggcttccg ccaggtcgaa cggcagtttg
    43561 cccatttcga taaaggtagc gaatgcacag gcacaaagtg ccagcaccag cgggatgctc
    43621 tggctcagcg gccagtgata aacggtgtcg gtgatgttgc tgatgttggt ggaaccggaa
    43681 acctgtgcgg caacccacag accaagcagc aacatcggtt cgaccagcac acccagcatc
    43741 gcttcacggc tcgcgccgat agcggtaaac gggctaccgg tatccagacc agaaatggca
    43801 aagaagaaac gcgcgatggc aaagagatac agtaaggtga tcaaatcacc cagttgcggc
    43861 agcggagaac caacggtcac caccggcagc gcagtagcga tagtcagcat tacgcccacc
    43921 atcacatacg gcgtcaggcg gaatacccag ccggaggcat ccgggccaac gctctggcga
    43981 cccagcagtt tgataatgtc gcgatactcc tgcaacacgc ccggcccgcg acggttatgc
    44041 aagcgggcgc gcgccacgcg ggtaataccg gagagcagcg gcgcaacggc aaataacacc
    44101 agcgcctgaa ttaacggata taaaacactc attctcaggc tcctcgtgaa acaataatca
    44161 ccaccagtac cgccagttca accagcgcca tccggcggaa cagcaacgca ctcccctcgc
    44221 actgccagcc cggcaccaga gacaccggat tcagccattt gcgtagtttc agcaccggcg
    44281 caaacgcctg tttcaccggc atggcaaaac cgtgagcggt aatcaccatt gatttttcgt
    44341 ggtcgtagcc gcacacccag gccgcaccgc gggaacgcga cggcaaacga tcgcctttgc
    44401 aaatcgccat aatgatgaat ggcagcagcg ggcaggcaat cagcagcaat gtgatcattg
    44461 gttgagaaac ggtggtgtta gctggctcca gcggcagagg tacagcagca gagagcatcg
    44521 gcagcagcca cggcgcagca acaccgccaa ttacgcagca aatcgccagt gccactacgc
    44581 ttacgctcat caggagcggc gcacaggtgg cgttttcggc ttctttggta cgcggcgcgc
    44641 cgaggaacgt gacgccatag actttcgcca tacacatcac cgccagcgca ccggtaattg
    44701 ccagacccac agcgagcagc ggtcccagca gacgggtaac aaacgcacca ctattgctca
    44761 gtttgaaaaa ggattgatag ataacccatt ccccggcaaa cccgttcagc ggcggcagcg
    44821 cagccattgc catcagcccg actaacatgg cgatggagat aaccggcatt ttcttgccaa
    44881 taccaccgag tttttcgata tcgcgatgac cggtacggaa ccagacgctc cccgccccga
    44941 ggaacagtac gcttttgaac aggctatggt taagcagatg gtacagacca ccgaccagac
    45001 caagggcaat cagcgccggt tgttcgagcg cgatacccgt tacgccagcg cccagcccca
    45061 gcaggatgat gccgatattt tccagggtgt ggtaagccag caggcgctgg atgttgtgct
    45121 ccatcagcgc atacagacca ccgacaaacg cggtgatcat gccgagcacc agcagcgcga
    45181 tcccccacca cagcggtgca ttaccgccca gcagtgacag ggttaaaatg cccagcaggc
    45241 caattttcat gactaccgtg gaaaacagtg cggcagctgg cgcagaggcg ttagcatgtg
    45301 cctgcggcac ccagccgtgc agcggaataa tcccggccag caggccaaag ccaatcacac
    45361 cgagcagcca gatatcggaa ccgagcggca gctgttgcat acgcatatcc agcaggcgca
    45421 gatccagcgt gccataacgc tgccacagca gccagcaagc aatcaccagc agcagagtgc
    45481 caagacgccc cagcgcaaac cacagtttgc cctctttgct gttgctggta aggaacaccg
    45541 cacacaaggc catgacttca gccattacca cgaacatgcc gaggttgctg gcgatgacgg
    45601 cgcagacggc ggcagccatc aacatattga tctgcaagcc gttgcacttc acctgcgcgt
    45661 ggcgatgcca gtcaatgtta tagaggctga caaacagacc gcacagaccg agcgtaatca
    45721 gccaaatcgc gttaagcgga gagatttgca catcgtagct taccagcgac agcgcaccgc
    45781 tcacgccaac cgcgccagtc agtacagtga agcccgcggc tgccgtatac aggctaccaa
    45841 ccgcgccgcc aattccggct atccagccac ttagcgcttt ttgaaaagaa aagagaaatg
    45901 ccagaacagc ggcggcgaca aaccacgcca cgccgctatt gatcagggaa attgcgctca
    45961 tttagcctct ccactttgag cctgctgaaa caaggtgaga tcgccaaagt ccgtgttaaa
    46021 ggtcagctca cgcttacgtt tgctgacgcg ggcgatatcg gtgttatcca ccagatgcag
    46081 ggctttggtc gggcacatcc gcacgcaggc cggaccttgt tcatcaacgc tacaaaggtc
    46141 acatttgacg gcgatcgcgc gaatacccgg cacccagtca agcagcgtac tgatacgcgc
    46201 cggagcaggc ggtgccggtg gcgctttcgg ggtattggcg tttgccggaa tatccagcgg
    46261 acggctgccg gaaaattcaa ttgcgccaaa cgggcaggcg atgccgcaca gtttgcagct
    46321 tacgcacagg ctttcattca actgcacggc cccatcaacg cgggtgatgg cgttaaccgg
    46381 gcagaccacc gcgcagggtg catcttcaca gtggtgacag agctgcggcg cagattcttt
    46441 ttcatttagc atcactctca ggcgcggcat tgattgcagg ccgtgctggc gatgcgtctc
    46501 tgaacaggcg gcctcacaag tgtggcagcc gatacagagc gtggagtcag caattacaaa
    46561 acgattcacc aggcattcct caggtgattg tcatttttga cgaaaacatg ccgatgaaat
    46621 gacattttcg acactcatcg acacgcccat ccccaaacag gcgtaacgcc tgcaaaacgg
    46681 gcaaagcctc agctcatgct gccgggcttt gtccctttac cagttggctt aaattcaccg
    46741 gcacattgtt ttcaatggcg gtgtataagc tgtcgtaaac gccagcgatt ttttcgagat
    46801 agtgttccag aacctgttcg cgatcgcgga tgttgacctt gccatcaatc atgccgtcaa
    46861 tgctcagttg cgcctgcgca atccgctgtt tgtcttcgcc atcttttgtt tcgacgtaat
    46921 actcgatggt gtggctgtta aagccatccg ccagggtgac gtaaatacga aaatgttcaa
    46981 aaaggacgaa acagagttct ttgtccggcg cgcagctcat ggcgtgatgc aggcgcgctt
    47041 tcgataacgt gatcccctga accagcgaat tgcagtagat gtgccactgg tcctgtaggc
    47101 gacgatgccg ctgtgcgatg taatcggctt tc
//
LOCUS       DAZGXZ010000019        46967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000019 DAZGXZ010000000
VERSION     DAZGXZ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.019"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..76)
                     /gene="ylcJ"
                     /locus_tag="ACHKJF_001222"
                     /pseudo
     CDS             complement(1..76)
                     /gene="ylcJ"
                     /locus_tag="ACHKJF_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518754.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
     gene            complement(125..457)
                     /gene="emrE"
                     /locus_tag="ACHKJF_001223"
     CDS             complement(125..457)
                     /gene="emrE"
                     /locus_tag="ACHKJF_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP0948617.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(525..827)
                     /locus_tag="ACHKJF_001224"
     CDS             complement(525..827)
                     /locus_tag="ACHKJF_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP0948618.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(824..1525)
                     /locus_tag="ACHKJF_001225"
     CDS             complement(824..1525)
                     /locus_tag="ACHKJF_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0948619.1"
                     /translation="MKNIAAQMVNFDCEQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1522..2541)
                     /locus_tag="ACHKJF_001226"
     CDS             complement(1522..2541)
                     /locus_tag="ACHKJF_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0948620.1"
                     /translation="MTGSEMAKAGLLEQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKEPRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNTGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKPKLDLTNTDWI
                     YGVEL"
     gene            complement(2538..3077)
                     /locus_tag="ACHKJF_001227"
     CDS             complement(2538..3077)
                     /locus_tag="ACHKJF_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP0948621.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQVPGHEKIRDAVRAWSAVDN
                     QDVVATLIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3147..3377)
                     /locus_tag="ACHKJF_001228"
     CDS             complement(3147..3377)
                     /locus_tag="ACHKJF_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0948622.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPKLFPHTEKNAA"
     gene            3482..4171
                     /locus_tag="ACHKJF_001229"
     CDS             3482..4171
                     /locus_tag="ACHKJF_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0948623.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSTVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            4767..4973
                     /locus_tag="ACHKJF_001230"
     CDS             4767..4973
                     /locus_tag="ACHKJF_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP0948624.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            5049..5345
                     /locus_tag="ACHKJF_001231"
     CDS             5049..5345
                     /locus_tag="ACHKJF_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0948625.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            5351..6136
                     /gene="bet"
                     /locus_tag="ACHKJF_001232"
     CDS             5351..6136
                     /gene="bet"
                     /locus_tag="ACHKJF_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP0948626.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            6133..6813
                     /locus_tag="ACHKJF_001233"
     CDS             6133..6813
                     /locus_tag="ACHKJF_001233"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0948627.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            6810..6971
                     /locus_tag="ACHKJF_001234"
     CDS             6810..6971
                     /locus_tag="ACHKJF_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFP0948628.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWAFPGLSVIRNPLKAQRLAEA
                     INNKRGLYD"
     gene            6964..7521
                     /locus_tag="ACHKJF_001235"
     CDS             6964..7521
                     /locus_tag="ACHKJF_001235"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0948629.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            7598..7813
                     /locus_tag="ACHKJF_001236"
     CDS             7598..7813
                     /locus_tag="ACHKJF_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0948630.1"
                     /translation="MTHQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            7912..8130
                     /locus_tag="ACHKJF_001237"
     CDS             7912..8130
                     /locus_tag="ACHKJF_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0948631.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            8178..8456
                     /locus_tag="ACHKJF_001238"
     CDS             8178..8456
                     /locus_tag="ACHKJF_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0948632.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            8428..8493
                     /locus_tag="ACHKJF_001239"
                     /pseudo
     CDS             8428..8493
                     /locus_tag="ACHKJF_001239"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            8655..9818
                     /gene="intD"
                     /locus_tag="ACHKJF_001240"
     CDS             8655..9818
                     /gene="intD"
                     /locus_tag="ACHKJF_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP0948633.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(9834..9910)
                     /locus_tag="ACHKJF_001241"
     tRNA            complement(9834..9910)
                     /locus_tag="ACHKJF_001241"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9874..9876),aa:Arg,seq:tct)
     gene            10153..10785
                     /gene="fimZ"
                     /locus_tag="ACHKJF_001242"
     CDS             10153..10785
                     /gene="fimZ"
                     /locus_tag="ACHKJF_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP0948634.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLSGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(10788..11303)
                     /gene="sfmF"
                     /locus_tag="ACHKJF_001243"
     CDS             complement(10788..11303)
                     /gene="sfmF"
                     /locus_tag="ACHKJF_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP0948635.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(11314..12321)
                     /gene="fimH"
                     /locus_tag="ACHKJF_001244"
     CDS             complement(11314..12321)
                     /gene="fimH"
                     /locus_tag="ACHKJF_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0948636.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(12334..14942)
                     /gene="sfmD"
                     /locus_tag="ACHKJF_001245"
                     /pseudo
     CDS             complement(12334..14942)
                     /gene="sfmD"
                     /locus_tag="ACHKJF_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(14973..15665)
                     /gene="fimC"
                     /locus_tag="ACHKJF_001246"
     CDS             complement(14973..15665)
                     /gene="fimC"
                     /locus_tag="ACHKJF_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0948637.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(15885..16427)
                     /gene="fimA"
                     /locus_tag="ACHKJF_001247"
     CDS             complement(15885..16427)
                     /gene="fimA"
                     /locus_tag="ACHKJF_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0948638.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            16908..17774
                     /gene="folD"
                     /locus_tag="ACHKJF_001248"
     CDS             16908..17774
                     /gene="folD"
                     /locus_tag="ACHKJF_001248"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP0948639.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            17776..17988
                     /gene="ybcJ"
                     /locus_tag="ACHKJF_001249"
     CDS             17776..17988
                     /gene="ybcJ"
                     /locus_tag="ACHKJF_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP0948640.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            18096..18617
                     /gene="ybcI"
                     /locus_tag="ACHKJF_001250"
     CDS             18096..18617
                     /gene="ybcI"
                     /locus_tag="ACHKJF_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0948641.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(18653..20038)
                     /gene="cysS"
                     /locus_tag="ACHKJF_001251"
     CDS             complement(18653..20038)
                     /gene="cysS"
                     /locus_tag="ACHKJF_001251"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP0948642.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            20212..20706
                     /gene="ppiB"
                     /locus_tag="ACHKJF_001252"
     CDS             20212..20706
                     /gene="ppiB"
                     /locus_tag="ACHKJF_001252"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP0948643.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            20709..21431
                     /gene="lpxH"
                     /locus_tag="ACHKJF_001253"
     CDS             20709..21431
                     /gene="lpxH"
                     /locus_tag="ACHKJF_001253"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP0948644.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            21549..22058
                     /gene="purE"
                     /locus_tag="ACHKJF_001254"
     CDS             21549..22058
                     /gene="purE"
                     /locus_tag="ACHKJF_001254"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP0948645.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPYHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATYDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            22055..23122
                     /gene="purK"
                     /locus_tag="ACHKJF_001255"
     CDS             22055..23122
                     /gene="purK"
                     /locus_tag="ACHKJF_001255"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP0948646.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(23259..24152)
                     /gene="ybcF"
                     /locus_tag="ACHKJF_001256"
     CDS             complement(23259..24152)
                     /gene="ybcF"
                     /locus_tag="ACHKJF_001256"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0948647.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(24149..24964)
                     /gene="ylbF"
                     /locus_tag="ACHKJF_001257"
     CDS             complement(24149..24964)
                     /gene="ylbF"
                     /locus_tag="ACHKJF_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0948648.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(24975..26234)
                     /gene="ylbE"
                     /locus_tag="ACHKJF_001258"
     CDS             complement(24975..26234)
                     /gene="ylbE"
                     /locus_tag="ACHKJF_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0948649.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(26244..27911)
                     /gene="fdrA"
                     /locus_tag="ACHKJF_001259"
     CDS             complement(26244..27911)
                     /gene="fdrA"
                     /locus_tag="ACHKJF_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0948650.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELTNQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            28228..29277
                     /gene="allD"
                     /locus_tag="ACHKJF_001260"
     CDS             28228..29277
                     /gene="allD"
                     /locus_tag="ACHKJF_001260"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP0948651.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLHARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFFSS
                     KLFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            29299..30534
                     /gene="allC"
                     /locus_tag="ACHKJF_001261"
     CDS             29299..30534
                     /gene="allC"
                     /locus_tag="ACHKJF_001261"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP0948652.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            30545..31330
                     /gene="allE"
                     /locus_tag="ACHKJF_001262"
     CDS             30545..31330
                     /gene="allE"
                     /locus_tag="ACHKJF_001262"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP0948653.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(31558..32712)
                     /gene="glxK"
                     /locus_tag="ACHKJF_001263"
     CDS             complement(31558..32712)
                     /gene="glxK"
                     /locus_tag="ACHKJF_001263"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP0948654.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGIGGSATVDGGMGMAQALGVRFLDAD
                     GQVLVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGAT
                     PEMVEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVL
                     NAVNLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVE
                     VVHQYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(32734..34035)
                     /gene="ybbY"
                     /locus_tag="ACHKJF_001264"
     CDS             complement(32734..34035)
                     /gene="ybbY"
                     /locus_tag="ACHKJF_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP0948655.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLLTVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(34092..35453)
                     /gene="allB"
                     /locus_tag="ACHKJF_001265"
     CDS             complement(34092..35453)
                     /gene="allB"
                     /locus_tag="ACHKJF_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP0948656.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAYTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFIGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(35513..36967)
                     /gene="ybbW"
                     /locus_tag="ACHKJF_001266"
     CDS             complement(35513..36967)
                     /gene="ybbW"
                     /locus_tag="ACHKJF_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP0948657.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLNYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(37136..38014)
                     /gene="glxR"
                     /locus_tag="ACHKJF_001267"
     CDS             complement(37136..38014)
                     /gene="glxR"
                     /locus_tag="ACHKJF_001267"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0948658.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(38114..38890)
                     /gene="hyi"
                     /locus_tag="ACHKJF_001268"
     CDS             complement(38114..38890)
                     /gene="hyi"
                     /locus_tag="ACHKJF_001268"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP0948659.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            complement(38903..40684)
                     /gene="gcl"
                     /locus_tag="ACHKJF_001269"
     CDS             complement(38903..40684)
                     /gene="gcl"
                     /locus_tag="ACHKJF_001269"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP0948660.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LHNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(40774..41589)
                     /gene="allR"
                     /locus_tag="ACHKJF_001270"
     CDS             complement(40774..41589)
                     /gene="allR"
                     /locus_tag="ACHKJF_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP0948661.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(41667..42149)
                     /gene="allA"
                     /locus_tag="ACHKJF_001271"
     CDS             complement(41667..42149)
                     /gene="allA"
                     /locus_tag="ACHKJF_001271"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP0948662.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            42379..43305
                     /gene="allS"
                     /locus_tag="ACHKJF_001272"
     CDS             42379..43305
                     /gene="allS"
                     /locus_tag="ACHKJF_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP0948663.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            43374..44468
                     /gene="mnmH"
                     /locus_tag="ACHKJF_001273"
     CDS             43374..44468
                     /gene="mnmH"
                     /locus_tag="ACHKJF_001273"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP0948664.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            44584..44949
                     /gene="ylbG"
                     /locus_tag="ACHKJF_001274"
                     /pseudo
     CDS             44584..44949
                     /gene="ylbG"
                     /locus_tag="ACHKJF_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(45470..45961)
                     /locus_tag="ACHKJF_001275"
     CDS             complement(45470..45961)
                     /locus_tag="ACHKJF_001275"
                     /inference="COORDINATES: protein motif:HMM:NF011298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948665.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFPHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQAAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFFVGEINVTHYFITNIGAGLPDA
                     CAE"
     gene            complement(45942..>46967)
                     /locus_tag="ACHKJF_001276"
     CDS             complement(45942..>46967)
                     /locus_tag="ACHKJF_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0948666.1"
                     /translation="QPGSFAPLIRIETDNGEREKAQCRSLAEKLQQEGSEDGHGVVFP
                     AELVRLLDRLEEEIRADRVSDESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDH
                     RGLPLALISEDGNTVWSAEYDEWGNQLNEENPYYLYQPYRLPGQQYDEESGLDYNRHR
                     YYDPLQGRYITQDPIGLAGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRA
                     WDILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLN
                     LFGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTIKAL
                     QDAGYLK"
BASE COUNT        12091 a        12180 c        11221 g        11475 t
ORIGIN      
        1 acgccataat cttgaaagca caaaagaaca cataaaaaaa taaagacatt aacaaaaagc
       61 ataaaacgag gctcatataa atataagagc ctccatattt tagtcgttta gaaacaaatt
      121 atttttaatg tggtgtgctt cgtgacaata gattaataac caacacaccg gcacaaatca
      181 acatcatgcc tataatggct ggcaggtcca gccgttggcc gaaaagtccc catgacagta
      241 agctaatcag gacaataccg actcctgacc agatagcata agcaatccct gtaggaatat
      301 aagccagcgt ctgagctaat aaccagaatg atgcacaata acaaataatt gtaccaacag
      361 atggccataa ccgtgtaaaa ccttctgaaa acttcattaa ggttgtacca atgacctctg
      421 caagtattgc accaccaaga taaatataag ggttcatagc atattctttc ctgttcaaac
      481 tggagagaat tgtactacag tttgaactca actcacctgt ttcatcattg tgtacccatt
      541 gatgttcttt tatataccct caatacccgt ttcatcgcgg cactctggcg acactcctta
      601 aaaatcagat tcgtgctcac ctttccttcc cgttcttctc tggtagcgaa ccggtaatac
      661 accgttcgcc agaccttacc atcaacgacc aggattcctg cccgcgccat tttagccgca
      721 gcctgattta tgctggttac ggttgcgcct gttaccgcgg caacgtcctg tgcacagaag
      781 ctcttatgcg tccccaggta atgaataatt gcctctttgc ccgtcataca cttgctcctt
      841 tcagtccgaa cttagcttta atttctgcga tcttcgccag agcctgtgca cgatttagag
      901 gtctaccgcc cataacagga agttgtttta ctggttcagg tatcgtctca ccacggttaa
      961 ttcgcgctgt catacaggtc agttcatcgg cagccttgcg ccgtaattcc gcgtcagtca
     1021 gcgcattggc ccgcatgttc tggtacaagt tggtaaccaa ccagtaatgc gcgttcgatt
     1081 tccacggata agactctgca tccggataca ggccacgctt ccggcaatac tcgtaaacca
     1141 tatcaaccag ctcgctgacg tttggcagcc cggcgttaac agatgcttct tcccggcacc
     1201 aggcgacaaa ctgcccgggt gatggcagga atggtcgatt ctgccgacgg gctacgcgca
     1261 ttccagcgtt aacctgttcc attgtggtga tcccgttttc ccggaaagcc agaacccact
     1321 ggcggcggat ttcgttcagt tcattctggt cacggttagc caggctcgcc gggaaagttg
     1381 ccagtaactg gctgaacaca ccgttgatga tctgcgctac ctgctgtacc tgtggctttt
     1441 cgtcgtactg ttccggcatg ttgttggcga tccggcgcat ctgctcacag tcaaagttaa
     1501 ccatctgtgc ggcgatgttt ttcatagctc caccccgtaa atccagtcag tgttcgtcag
     1561 gtcgagtttt ggtttgccgg ctgtcacgcc agcctgttgc ttgtttcggt tgatttcgag
     1621 ctgggtccac ttgtcgcgga gtttggccgg acttagcacg ttaccggacc agaagttgtc
     1681 ctggcatgcc cagcggaaca gcacgcacat gtcgcggtgg ttacgtccgt cacgttcacg
     1741 catcaggcgg atatcgttag cccaccctgc aaaattcggt tttctggctg atggcgcgat
     1801 ggtcttcacc atgtcaaaca tccactctgc ggcggtcagg tcttctgctg tcccccactt
     1861 gctgccgctc tgaattgcag catccggttt caccacagga agatcgtttt ctggttggtc
     1921 agaggattcg ccagaattct cggacgaaaa aggttttata ttgtcttttg ttagtttgtc
     1981 ttttgtgttt acctgattcg ggtaaacgcc tttacctgat ttgggtaaac tttttttacc
     2041 tgattcaggt aaatttacct ctttcaggta aactttattt ttcttacctg attcgggtaa
     2101 tgttgaccat tcactgacca cattattaat gccggtattc cgcccgctct gaataaaaat
     2161 cccacgcttt accagaacac tttttgcagc agaacacttg tgcggcaata tcccggttaa
     2221 ttcggaaagt tgctcgttgc taacccaatc cagtttttta ttaaagccat atgttttgcg
     2281 catgacagcc agaaagacca gaagctggtg ctgtgttaat ccggccagca tcacagcttc
     2341 cagcaactca tttgcaatgc gcgtataacc atcatcgaga tctgccacgc gcggctcctt
     2401 ttgtgccgca tccggcactg gaaaattgaa tatctcagca gtgtttgcca taattcctcc
     2461 cgcaatgagt gtgttacgat ttgcacctga aagtcggttc tgttccagca gaccggcttt
     2521 cgccatttct gaacctgtca tatcgccccc agcatggtag taaccatcgc catcaatgga
     2581 ccagccagat ctgggtccac acgaaacatc gacacaatac cttcactaat ttccttcagt
     2641 ttctggtggc gtggtgcgtt gagaatgaca gcctgttttg cctcactgag ttccttttcc
     2701 atttcagcca acctagccat gaagctatcc tgctcaacca ggtaaccgcg atattccagc
     2761 ggtagtaccg ccagaattgc cggggtcagt tcacgcacgt tatttcggta tttttcagaa
     2821 tcgaatttgt tatcgaggaa gcggaacagc ttctggcgtg cacggctgac atcatcaggg
     2881 aaatcgatgg tgccgccgcc ctgctcccga tactcattca caatgagtgt ggcaacgaca
     2941 tcctgattat ctacagccga ccaggcgcgg acggcatcac ggattttttc gtggcctggc
     3001 acctgttttg tttgagaacg atttatcacc gcagtcgggc taaatccgct agtctgttgg
     3061 tatgtaagtg gttgcataat tgactccttt agtttgaatt gactgttaag ttgattgctt
     3121 attgttaaag agcgtgaaat ggaaatttaa gctgcgttct tttcggtgtg tggaaacaac
     3181 ttcggaagat ccgggcgaat ctggtatgcc ttcactactc caccagtagc cgtaacaatg
     3241 ctgccgacat gttcagggga tacctttgct ttgttgtgaa gccacttata gacggcctgc
     3301 tgtgaaactt cgcaagcagc gcccagtttc ttttgtgaac caacgatatt gatcgctgtt
     3361 ttgatagctg ggttcataac aacctccgtg gttaatttga atcaagatta aaaccatggt
     3421 tgtttttagt caacaaccat tttcgtttga tgaaataaaa ccttggttgt acatttggac
     3481 tatgaaaaca acactctcag aaagacttaa agaagccaga ttagcgcgag gccttacaca
     3541 aaaggcgctt ggggatttgg tcggggttag ccaggctgct attcagaaaa tcgaaacagg
     3601 gaaagctaat caaacaacta aaatcgtgga gatcgcgaac gctttgggtg tgcgcgcaga
     3661 atggttatct tctggcgttg gaaatatgtc agacagtaca gtgcaaccaa tacaatcaac
     3721 tgtcagccat tccaaatact tcaagattga cgttcttgat atagaagtca gtgctgggcc
     3781 gggagtcatc aaccgtgagt ttgtagaagt tctacgctcg gttgagtact cgtttgacga
     3841 tgctcgtcac atgttcgatg gtaggaaggc ggaaaatatc cgcatcatta acgtgcgtgg
     3901 tgacagcatg tcaggaacga tcgaaccagg tgatctgctg ttcgttgata tcacagttaa
     3961 atctttcgac ggtgatggta tctatgcgtt tctgtacgac gacacagccc atgtaaagcg
     4021 cctgcaaatg atgaaggata agctgctggt catctctgat aacaaaagct actcaccgtg
     4081 ggacccgatc gagaaagacg agatgaaccg ggtgttcatc ttcggtaagg ttattgggag
     4141 catgccgcag acatatagga agcatgggta gtaccaatta aaaattatca actgggcatt
     4201 gtgctcattc agtaaagaac taattcctat ctttgctcta ggtagtaata ttaagccacc
     4261 gcaataatat ctttacctaa cggcgtaaga atcccggtca ccgtgccggg ttttcttttg
     4321 ccctcccctc atcacacaca ccgttaaaaa aaccaccata acctcgcttc agttatcgct
     4381 atgcgattca agtcacaaaa taaatccatc ctaaatacaa ccagttatat ctaaaacaac
     4441 caataaaaca acttttgttg ttgacgataa aacaactata gttttaaata agttcatcgc
     4501 aacaacacaa cgatacggca actacctgat tcaccgttgc gatgaccgct tagatccgca
     4561 gtttgaattt cagcaggctt cggggagtgc gaggggtgaa acggacgcgt gaacgtcggt
     4621 gtgaccagct gaaattaact caacatttca taccttagtc gcttcaacga ggcggcttag
     4681 ttatgacaac cggcggccat ccaccgcctg aatacgcgca gaagtctcta tatgttcagc
     4741 agcccagctt acgggcagga gtttttatgg ttcatcaaca ttatggaacg cagaccgtta
     4801 atcgaggtgc ggtcatgcca ggaatgctgg tcaaacacaa agatggtacc tggactgcat
     4861 cagctaattt acgcggacgg ctttatctgc atcgcggcat cgagcgcact tatacccgtg
     4921 atttgctcgt ggaagttttt ctcgacggac gcggtaacgg cctgaatcac taatcccctt
     4981 tcctgttttc ctaatcagcc tggcatttcg cgggcgatat tttcacagcc attttcagga
     5041 ggtcagccat gaacgcttat tacattcagg atcgtcttga ggctcagagc tgggcgcgtc
     5101 actaccagca gatcgcccgt gaagagaaag aggcagaact ggcagacgac atggaaaaag
     5161 gcctgcccca gcacctgttt gaatcgctat gcatcgatca tttgcaacgc cacggggcca
     5221 gcaaaaaagc cattacccgt gcgtttgatg acgatgttga gtttcaggag cgcatggcag
     5281 aacacatcca gtacatggtt gaaaccattg ctcaccacca ggttgatatt gattcagagg
     5341 tataaaacgg atgagtacag cactcgcaac gctggcaggg aagctggctg aacgtgtcgg
     5401 catggattct gtcgacccac aggaactgat caccactctt cgccagacgg catttaaagg
     5461 tgatgccagc gatgcgcagt tcatcgcatt gttgatcgtc gccaaccagt acggccttaa
     5521 tccgtggacg aaagaaattt acgccttccc tgataagcag aacggcatcg ttccggtggt
     5581 gggcgttgat ggctggtccc gcatcatcaa tgaaaaccag cagtttgatg gcatggactt
     5641 tgagcaggac aatgaatcct gtacatgccg gatttaccgc aaggaccgta atcatccgat
     5701 ctgcgttacc gaatggatgg atgaatgccg ccgcgaacca ttcaaaactc gcgaaggcag
     5761 agaaatcacg gggccgtggc agtcgcatcc caaacggatg ttacggcata aagccatgat
     5821 tcagtgtgcc cgtctggcct tcggatttgc tggtatctat gacaaggatg aagccgagcg
     5881 cattgtcgaa aatactgcat acactgcaga acgtcagcca gaacgcgaca tcactccggt
     5941 taacgatgaa accatgcagg agattaacac tctgctgatc gccctggata aaacatggga
     6001 tgacgactta ttgccgctct gttcccagat atttcgccgc gacattcgcg catcgtcaga
     6061 actgacacag gccgaagcag tgaaagctct tggattcctg aaacagaaag ccactgagca
     6121 gaaggtggca gcatgacacc ggacattatc ctgcagcgta ccgggatcga cgtgagagct
     6181 gtcgaacagg gggatgatgc atggcacaaa ttacggctcg gcgtcatcac cgcttcagaa
     6241 gttcacaacg tgatagcaaa gccccgctca ggaaagaagt ggcctgacat gaaaatgtcc
     6301 tacttccaca ccctgctggc tgaggtttgc accggtgtgg ctccggaagt taatgctaag
     6361 gcgctggcct ggggaaaaca gtacgagaac gacgccagaa ccctgtttga attcacttcc
     6421 ggcgtgaatg ttactgaatc cccgatcatc tatcgcgacg aaagtatgcg caccgcctgc
     6481 tctcccgatg gtttatgcag tgacggcaac ggccttgaac tgaaatgccc gtttacctcc
     6541 cgggatttca tgaagttccg gctcggtggt ttcgaggcca taaaatcggc ttacatggcc
     6601 caggtgcagt acagcatgtg ggtgacgcga aaagatgcct ggtactttgc caactatgac
     6661 ccgcgtatga agcgtgaagg cctgcattat gtcgtgattg agcggaatga aaagtacatg
     6721 gcgaattttg acgagatggt gccggagttc atcgaaaaaa tggacgtggc actggctgaa
     6781 attggttttg tatttgggga gcaatggcga tgaagcatcc tcacgataat atccgggtag
     6841 gcgcgatcac tttcatctac tccgttacaa agcgaggctg ggcatttccc ggcctttctg
     6901 ttatcagaaa tccactgaaa gcacagcggc tggctgaggc gataaataat aaacgggggc
     6961 tgtatgactg atttcaccgg aagcaatact cctgccgaac atcgcgacag ctggcgcaca
     7021 ccaccagaga tttttgctgc gcttaatgca gagttcgttt ttcaacttga tgctgccgcc
     7081 agcgaaaaaa accgactatg tcggcttttt atctcacagg agcagaacac attaaccact
     7141 tcatggcctg aagcaatggg atatgcctct ggttatgtct ggttgaatcc accatacagc
     7201 aatatttccc cttttgtgaa aaaggcagcc actgaaaaca aattcagtag tgtgggatgt
     7261 gtaatgttat tgcctgctga cacatctgtc ggatggtttc atgaagcgat acaaaccgcc
     7321 agtgaggtca gattcatcac ggcaggacga ctggcattta ttaacccact cactgagaaa
     7381 cccgtcagtg gaaataataa aggctcgatg ctcattatct ggcacccata cccccgtaca
     7441 cactgccact ttacgaccgt tgatcgtgga gagttgatgg cgttcggctc aaggattctt
     7501 gcccgtcggg aggctgcatg acaaccacgg aatgcatttt tctggcagcg ggcttcatat
     7561 tctgtgtgct tatgcttgcc gacatgggac ttgttcaatg acacatcagc aggaaaacgc
     7621 ccttcgcagc attgcccgtc aggctaattc tgaaatcaaa aaagccagac agcagtttcc
     7681 ggataaaaac gtcgatgaca tttgccgtag cgtacttaag aagcaccgcg aaacggtaac
     7741 gctgatggga ttcacaccga ctcatttaag cctggcgatc ggcatgttaa acggcgtctt
     7801 taaggaacgg tgaacatgaa aagcaaaatc atcagggagc tacaggctcc ttttttattg
     7861 ttcgcattca tcctcaagcg tattaaccaa caattcaggg attaatggaa gatggcagac
     7921 atcattgatt cagcatcaga aatcgaagaa ttacagcgca atacagcaat aaaaatgcgt
     7981 cgtcagaact accagactgt atccgcaact cattgttgtg agtgtggcga tccgatagat
     8041 gagcgaagac gcctggctgt tcagggttgt cggacttgtg caagttgcca ggaggagatc
     8101 gaacttaaga acaaacaatg gggactgtga tggcctcaaa gcagcaaatt tcaacatcgt
     8161 ccaactgagg tgtaaaaatg ttcagaatca tttttcctaa cacctggtac gtcgaccacc
     8221 acggcactcc ctgcaaaatc ctgcgttcta cccacaacaa agttcactac atccgaaaag
     8281 gcagaacatg tatcgccagc atgttccgct ttaatcatga ctttgaacct gtgaataaag
     8341 ctgatgcaga tcggatagca gaagagatcg aaacggcaga acacattaag aagttacgtg
     8401 acatgcgttc aaaaagcaga ggtaaccatg gaatcataca gcctcacact cgatgaggcc
     8461 tgtcagtttc ttaagatatc cagaccaacc gccaccaact ggatacgaac aggccgccta
     8521 caggcaacac gtaaagatcc aaccaagcca aaatctcctt acctcacaac acggcaagcc
     8581 tgcattgcgg cgcttcagtc tccgctgcat actgtccagg tgagcgcggg tgatggcata
     8641 acagaggaaa gaaaatgtca ctcttccgca gaaatgaaat atggtatgcc tcgtattcgc
     8701 tcccgggcgg gaaacgaatt aaggaatctc ttggcacaaa ggacaagcgg caagctcagg
     8761 agttgcacga caagcgaaaa gcagaactct ggcgagtaga aaagctaggg gatttacctg
     8821 atgtcacttt tgaagaggcc tgcctaagat ggcttgagga aaaagctgat aaaaaatctc
     8881 tcgattcaga taaaagccgg attgagttct ggcttgaaca ttttgagggt ataaggctta
     8941 aagatatctc ggaggcaaag atttactctg ctgtaagcag aatgcataac agaaagacga
     9001 aagaaatatg gaaacagaaa gttcaggccg ccatcaggaa aggtaaagaa ccgcctgttt
     9061 atgaaccaaa gccagtatca actcagacaa aggcaaagca tcttgccatg ataaaggcca
     9121 ttctccgtgc tgcagaacgc gactggaagt ggctggaaaa agcgcctgtc atcaagatac
     9181 cagcggtcag aaacaagcga gtcagatggc tggaaaagga ggaagcaaaa cgccttattg
     9241 atgagtgccc cgaaccactg aaatctgtcg tcaagtttgc gctggcaact ggtctgagaa
     9301 agtcgaacat cataaatctg gaatggcaac aaatcgacat gcagcgacga gttgcctggg
     9361 tgaatccaga agagagcaaa tcaaaccgcg ccattggtgt ggcgctgaac gataccgcct
     9421 gtaaagtgtt gcgtgatcaa ataggcaagc atcacaaatg ggtgtttgta cataccaagg
     9481 cggctaagcg agcagatgga acatcaacgc ctgcggtcag gaagatgcgc atcgacagca
     9541 agacatcatg gctatcagct tgtcgtcgtg caggaattga agatttccgt ttccatgacc
     9601 tcagacacac ctgggcaagc tggctgattc agtcaggcgt cccattatca gtgcttcagg
     9661 aaatgggcgg atgggagtcc atagaaatgg ttcgtaggta tgctcacctt gcgcctaatc
     9721 atttgacaga gcatgcgagg aaaatagacg acatttttgg tgataatgtc ccaaatatgt
     9781 cccactctgg aattatggag gatataaaga aggcgtaact gattgaattg taatggcgcg
     9841 ccctgcagga ttcgaacctg cggcccacga cttagaaggt cgttgctcta tccaactgag
     9901 ctaagggcgc gttgataccg caatgcggtg taatcgcgtg aattatacgg tcaacccttg
     9961 ctgagtcaat ggcttttgat ctggttgctg aacaagtgaa cgaccgcgtc tgattttctg
    10021 atttatttcg ctatagcggc aaacaaacac acaccgctgc gcgtctgaat caagaaaacc
    10081 cgtattttca tgtatcaaag tacaatttac cgccctaacg gaaaattgtc cgctcctatg
    10141 agactggtaa ctatgaaacc aacgtcggtg atcattatgg atactcatcc tatcatcaga
    10201 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
    10261 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
    10321 atagacttgt ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
    10381 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
    10441 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatgcc
    10501 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgcttaa ctatataaaa
    10561 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
    10621 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
    10681 agcaataaaa cagttagtgc gcataaatct aatatttatg gcaagctagg tttgcattca
    10741 attgtagagc ttatcgacta cgccaaatta tacgaattaa tataatatta attataattg
    10801 atcataaata tcgcatccgc tttcgccaca cctggccgaa caccgctggc taacgctcga
    10861 taattggcaa aaaagtttag tgtgacattg ccatttgcat ctacttcctc agtcgggctc
    10921 gcctccccca gtgcgagccg ggagcgatcg ctattacgta attcgatggc gacggtttgt
    10981 gccattgcgg gatcatccag agccagcagg ttggtatcgg atgccggcgt tcccgtaaat
    11041 aaaatcgcaa ctgaacccgg aggacatccc tccagccgca ggctaaaagg gacgagtgcc
    11101 gtggtatcgc cagcgttcag taattgtgtc gtaggccatc tgcctaaatc taccgtctta
    11161 tcaatatccg cagtgtttac ggtacaggag aaatcaacaa cgttaccgtg caaattgata
    11221 ttaatcgttc ccaaagggtc agctgcccat ccactggaaa tccacagtaa cccgcagaaa
    11281 cagctaaaga gtgctcttct cattctccgt acctcattga taatcgacgc gtaaataccc
    11341 cagcgcgcta aacggccctt cggtcggttt ttgaccggta atactgatag gccaggcgcg
    11401 aagtgtgaca ttggctgccg cagctgcatc cagacggaaa ggaataacgc tattgagatc
    11461 gttaggcgtg atcggcgtat cgttctgatc ggcgacaata aaacctaaat cctgattgtc
    11521 cgacaccatc gcctgaccag aaacggcact ggcttccaga cgcattgtta aataagcctg
    11581 cgcagcaaca ttcgtacatt tgaccgcaat gctcttggtt tgcggcatga caccagcagg
    11641 tcgattaccc ggccctgccg cactaaataa cgatgcgccg atatcaccaa aatcaaattc
    11701 aacaatctgc ccggcattta attcgcagtt ttgcggtact tcaacccggc caccaaaact
    11761 aatggtataa acaggtgtaa ccaacggatc gtaaggcgtt gatgtcacat acacggtaaa
    11821 catcacctgt ctggggatct ccaccatgtt aataaaggga cgaataacct ttaaacgaaa
    11881 tattaaacga gaatccatca ccccgaatgg aaggttttgc gaaacgttag gatctgttcc
    11941 catcagaata taattttgcg gggggaactg aatatccatc acactatcaa ccagactcat
    12001 cgcacctaat agataatcat ttaattgcat atatttataa ttatcgatag tttcctgaac
    12061 aataaagttg gtgacataac tacggtatgt ataattcacc agcgtaccgg gtggacaaat
    12121 tgctgagaca cctacccagc cggatttttc cggtaaaaca acaatctgcc ctggctggtt
    12181 attggtgcta ttaaagatat ttgagagatc atagtgttca tcttctgcta cgccagtact
    12241 atttgcacaa acagtagccc aggatatgtt tgccagacac aaacatgcca tcgccaataa
    12301 taatctgcag attatcttca ttgataattt cctttatgag ccaggatgtg tgcaaactgc
    12361 cgaaataaca gtgacggctt gctgtaagct ttgcttcggc aatacatagt gcgcaatgca
    12421 gcgtgagttc gcgccgtctc cccactgaac cagtaattca ccagacaatg gcgcaccact
    12481 taaataaact tgcccgtcat cacccacata ctggttattc cgatactgtt ttcccgtacc
    12541 agtgatccaa acggcaccgg ttttccgccc tgggtaacgg taatgagcgc ccgcacgcct
    12601 atgcgggtat caaaattggc acgaaccaac gcgccttgcg tcggcacaac gctgctaata
    12661 tttttttcaa catcgatgga attccccatc gtattggtat caagcgcgat acggttatac
    12721 cgataaaccg tggcatacgg catcaccgca tagccgcgcc agtcggttaa aatgccagtt
    12781 tgattttcaa tgcgtacacc gcctgcgcca ggcgctttaa tcaaaacatt ggtatcccct
    12841 aaaggctggc tcagcgttat gccattttca tgcccgacca caccgccaga cagctgccag
    12901 ttaacgtcat gttgatcgcg gtcgtagtta tacccgaccc ccagcgtagc gtaagcggcc
    12961 tgccagtttg ccgtggcgct gcccgtatac ccattattcg aggtatcacc ctggcttacg
    13021 tgataactca ggttgtggcc ttccagtaag gtcccaccta cacctgccag ccagctattt
    13081 tgcccgttgc tgttacggtt ggcgttaaag gaggcataag cgcggtcgag cgcattttcc
    13141 cgggtgtagc gacgtttggt cagaacattg aaaggcactg aaacattaag tccgacaata
    13201 cgttcgttat cggggatccc tacagattca ttccacgaaa atgagagtga ataactgatg
    13261 ccaacccagc tgctgatata ccccacctga taccaggtat ctgaatccga agtattccag
    13321 tatttttgat gggtaccaga gatataaagc gagccaaagt cattaagtga ttgtgaaata
    13381 tttaactgca aacggtcttt acggctaaag cgtaaattgt ggtaattcac gattatcggt
    13441 tcatcccgat gctcaccgtc gtaatcgtaa tcatattcgt acccctccat tcgacgatac
    13501 gcaacatcat caagggtata aaaaccttgt gtcgaatagc ggtaacccat taactgaaaa
    13561 ttggtgccga aggtgttcat cgatttcgca tagaggaagc gaatggaatc cccctcatga
    13621 cgactgtcgt cggctaactg actgcgcgca tgggtcacat ccagcgacac tgccccccag
    13681 tttccgagat tgcgccccag tcctaataaa aaggcggtgt aattggcaga taattgcgtc
    13741 ccgccgtagg cagtaaattc ctgtggtaaa ccgccgagtg ccgtgccctg aaaaaagaaa
    13801 ggcgatgatt gctgactatt accgctacga aaatcgcccg ccgtcaggtc aaatttgaaa
    13861 cgcccttcgc gttgtaaaat tggcactgtt gaatacggga tagtgtaatt ctgctgatta
    13921 ccatcgcgct cgtcgatggt gacgtccaga tcgccatttg aagatgtcgg gtgcaaatct
    13981 gtaatttcaa aagcgccggg ggaaacatag ctttgataga taataaaacc attttgccga
    14041 atcgttagct gggccgccgt acgggcaatc ccacgtaccg ttggggcaaa cccttgctgg
    14101 ctatcaggat acatattatc ggaagaataa agccgtacac cacgaaatcc aacaccatcg
    14161 aaaatatcac ttcctgtatt gccgtctccc attaccagtt cacttttcag cggaataatg
    14221 gcgcgctgta cccaggtgcc aatattattc cactgttctg aatgatatcc atttccgcga
    14281 aaatagttcc aggaaccatt gttgcgtaaa cgccacgggc caatattaat cccgctgagc
    14341 tcactaaaaa aatagctatc gttaccatta cctctgttac cggtgaaatt ataattcagg
    14401 agtaaagcag gaataccttc atcccactct tcaggcggaa tgtaaccgtg agcgctactc
    14461 aacaacgtta tctgaggaat agtgatgttc aggcgcatcg ctgcaaaatc aaagttaatt
    14521 gtggcatcag gtacagcttt cagtaaattg atgcatttat tgttttgctg ctgggctaac
    14581 tcgggaaatg cactgctatt aaggccaatt cgttcaagga gtacctggtt aaaacagggc
    14641 attaacccac ctgatttatc acctgtattt tctgttgtcg attcaaatac gatatcctgt
    14701 gaaccaatga actcatcatt acgccagaga tccacccgat acactcccgc aggttgatga
    14761 ttgcctctct caaagcgcga taaatcagca acggatgtgc cattctctaa taaaaatgcc
    14821 gggttgaaat aactttccgc atagcttaaa aaggagggta aaatggcaag agatgaataa
    14881 caaattccgg ccagacacca ggtatacctc tgcggaatat ccgtagtagt gggtattttc
    14941 ataattagct ctgatattgt cagatgattc ggttagttta agttcacttc tctgaccggg
    15001 gttactgaac cataatcatt aacggtctga aatttcagct ttccctgcac tcctgagggt
    15061 aagggaattt gtgaactaac tctgggtgcc gccatagcat tccccaactt ttggctgccc
    15121 acttgcaggt taacgagggt gacataaaaa ggtgatggat tatgaacatt gatatagtta
    15181 ccggatcgcg aaaacttgag tgtgtccggc gcttctgcgg gtaattcttg taattgaatt
    15241 ggacggagaa ataatttcat gcgcgataaa atcgccagtt gcaaaacatt cctgccgttc
    15301 aatgcatttt tatctaccga agggatcgtc ttaacattca tccagaacag agactcacga
    15361 tctgctgcca gcggtggacc ggtgtaaata atacgcaaag tattttcgct tttgggttca
    15421 ctaacaaaca gtggcggtgt aatgatgaat gacttttctt ttacaccgct gctgttttca
    15481 atccacgaat tgaccagaaa acgctcattg gtatggctat ttctaatcca taccgcagtc
    15541 tgtttagcat cagcgggata aataatacgc gtggcaccta atgcgatccc tccggcagca
    15601 tgggcgctgg ccgaaatgat taaataaaat ataatgagca tcaataactt tattttagtc
    15661 atcatagtta atacgccttg tgtattcctg acggcaaaaa taagaaatat aaagaaatga
    15721 ttgctttggt ttcttacaac acatcattcc cttttcattt aataaagcgg catgatacaa
    15781 agtgaacatg atttcgattt acattgccac cataccgctt cagacaaccg gtacattact
    15841 ttgttataag acgtgatata taattcaaaa caacgtggtt ttgattattc gtatctcata
    15901 ataaaagtcg cgtcagcatt tgcttgccct gctgatgcac ttgtaccggt gcctttataa
    15961 cgtgcagaaa aatgaagaac gttggttccg gggatcaagt tctggttggt tgagaagcta
    16021 ttcccatcag gtttgagaat tgcggatgta ttatcaagta tttcaatacc gacacccgtt
    16081 gctgttgttg tattggtact ggatgcaatg gccaataaat tatcattgat tgcatcagcc
    16141 tgaccagaaa atgcaacggc agcattagcg gcaacaacag gatcgcagtc gttcaactga
    16201 atggtgaatg gaattaatgc agaggtatta ccaacagcat tgaaaatatc ggtacgatat
    16261 tgaccgagtg tgacaacctg gtctgccgag tcagtattca ctgaacaggc agcattcacc
    16321 agttcgcctt caaagtgaat tgtaccgcca tctacaacgg cagcataact acccgtagcg
    16381 gcgaatagtg cggcagccag cgcagacgaa ataaatctta atttcatata tattccttca
    16441 atctcattta tcgactccac atccgtatat aaccgattac tttatttaag acactgatag
    16501 tagtaaaatt cctttttatc ctctaagaat gtcttaattg aaaatatgca ctctattcta
    16561 aaaaatagag agccccgtta gatgaatact tccgcgcaaa atatattcaa caaaaatata
    16621 gacctgaagc ggtaaattac caggctgaaa attcttttta tattgtcagg tatttcttaa
    16681 attatcttaa tccttaaaca aggaaataaa tcagttccag atttacaacg ccatcatgga
    16741 cgaaaaatga agctttcaat ctcagcgacc tcagcgacgg tgcgcctaac cttcgcaaga
    16801 ggtcgcttca cgcgataaat ctgaaacgaa acctgacagc gcgccccgct tctgacaaaa
    16861 tagacgcatc cccttcgacc tacgtaacag atggaatcct ctctctgatg gcagcaaaga
    16921 ttattgacgg taaaacgatt gcgcagcagg tgcgctctga agttgctcaa aaagttcagg
    16981 cgcgtattgc agccggactg cgggcaccag gactggccgt tgtgctggtg ggtagtaacc
    17041 ctgcatcgca aatttatgtc gcaagcaaac gcaaggcttg tgaagaagtc gggttcgtct
    17101 cccgctctta tgacctcccg gaaaccacca gcgaagcgga gctgctggag cttatcgata
    17161 cgctgaatgc cgacaacacc atcgatggca ttctggttca actgccgtta ccggcgggta
    17221 ttgataacgt caaagtgctg gaacgtattc atccggacaa agacgtggac ggtttccatc
    17281 cttacaacgt cggtcgtctg tgccagcgcg cgccgcgtct gcgtccctgc accccgcgcg
    17341 gtatcgtcac gctgcttgag cgttacaaca ttgatacctt cggcctcaac gccgtggtga
    17401 ttggcgcatc gaatatcgtc ggtcgcccaa tgagcatgga actgttgctc gcaggttgca
    17461 ccactaccgt gactcaccgc ttcactaaaa atctgcgtca tcacgtagaa aatgccgatc
    17521 tgttgatcgt tgccgttggc aagccaggct ttattcccgg tgactggatc aaagaaggcg
    17581 caattgtgat tgatgtcggc atcaaccgtc tggaaaatgg caaagttgtg ggcgacgtcg
    17641 tgtttgaaga cgcggctaaa cgcgcctcat acattacgcc tgttcccggc ggcgttggcc
    17701 cgatgacggt tgccacgctg attgaaaaca cgctacaggc gtgcgttgaa tatcatgatc
    17761 cacaggatga gtaacatggc gacattttct ttaggtaaac atccgcacgt tgagctgtgc
    17821 gacttgctga aactggaagg ctggagcgaa agcggcgcgc aggcgaaaat cgcgattgcc
    17881 gaaggccagg tgaaagtcga cggtgcggtt gaaacgcgca aacgctgcaa aatcgtcgcc
    17941 ggtcagacag tgagttttgc aggtcacagc gtacaggttg ttgcctgatc cctttcccgg
    18001 cctgtatcaa caggccggtt ctcttttcag acttttgccc tctacgcccc gttcatccat
    18061 gctgatagca tcgacatttt gccgaagcga ccatcatgcc aaccgttatt acgcacgccg
    18121 ccgttcccct atgcattggt ttagggttgg gttcgaaagt catccctccg cgtctgttat
    18181 ttgccggaat catcctggcg atgctgcccg acgccgacgt attgtcgttt aaatttggcg
    18241 ttgcttacgg caatgttttt ggtcatcgcg ggtttaccca ttcgctggtg tttgcgtttg
    18301 ttgtcccgct gttatgtgtg ctgattggac gccgatggtt cagggctggg ctgattcgct
    18361 gctggctgtt tttaaccgtc tcgttgctat cgcacagctt gctggattcg gtaaccactg
    18421 gcggtaaggg cgttggctgg ctgtggccgt ggtcagatga acgctttttc gctccctggc
    18481 aggtgattaa agtcgcgccg tttgcattgt ctcgttacac cacgccgtac gggcatcagg
    18541 tgataatttc tgaattgatg tgggtgtggt tgccggggat ggtgcttatg ggaatgttgt
    18601 ggtggcgcag gcgataaccg gatgcgaaaa ctcgcatccg gcaacagcgc aattacttac
    18661 gacgccaggt ggtcccttgc gggccatctt ccagcacgat ccccatctcg ttaagacggt
    18721 cacgcgccgc atctgccgcc gcccagtctt tcgctttacg ggcatccaga cgctgttgaa
    18781 ttaacgcttc aatctcagcc acttcgctgt cgtctgcctg cgcgccgctt tgcaggaacg
    18841 cttccggttc ttgctccagc aggcccaata cagcggaaag tttacgcagg tgagatgcca
    18901 ttgcattcgc cgctgccata tcttctgctt tcagacggtt tacttcacgc gccatatcaa
    18961 acagtacgga ataggcttcc ggggtgttga aatcgtcgtc catcgcttca ataaagcgcg
    19021 cttcaaacgc ttcgccaccg gcaggcgcaa cggttttatc tgtgccgcgc agcgcagtgt
    19081 agagacgctc cagcgccgca cgcgcttgct tcaggttctc ttcgctgtag ttcaactggc
    19141 tgcgatagtg gcccgacatc aggaagtaac gcacggtttc cgcgtcgtag tatttcagca
    19201 catcgcgcac ggtaaagaag ttacccagcg atttggacat cttctcgcgg tcaaccatca
    19261 ccatccccga gtgcatccag tagttcacat actggccatc atgggcacag gtggactgcg
    19321 cgatttcgtt ctcgtggtgc gggaacatca ggtctgaacc gccgccgtgg atatcaaagt
    19381 ggttacccag ctgcttgcag ttcattgccg aacattcaat gtgccagcct ggacggcccg
    19441 cgccccacgg agacggccag ctcggttcgc cctctttcga catcttccac agaacgaagt
    19501 ccatcgggtt gcgtttgtcg tcgaccacgt caacgcgcgc acctgcctgc agctggtcga
    19561 gatcctgacg cgacagcacg ccatatgtcg gatcggtcgg gacgtcgaac atcacgtcgc
    19621 cgttgtccgc cacataagcg tgacctttgg cgatcagttg ttcagtgagt tcaataattt
    19681 ctgcgatatg gtgcgtcgcg cgcggctcca tatccgggcg cagaatgttc aaagcatcaa
    19741 aatctttgtg catttcggcg atcatgcgat ccaccagcgc cacaaagctt tcgccatttt
    19801 cattggcgcg tttgatgatt ttgtcgtcga tatcggtaat gttgcgcaca tacttcagtt
    19861 tatagccgag gaaacgcaga tagcgcgcaa ccacgtcaaa agcaacaaag gtacgcccgt
    19921 gaccgatatg acagagatcg taaacggtga ttccacacac atacatgccg acttccccgg
    19981 cgtgaatagg cttaaattcc tctttttggc gtgtcagagt attgaagatt tttagcatcg
    20041 aagattccgt ttagacatgt gtgggtaatt gagttgcgta taatacccat atttcccgcg
    20101 cgaatcagca tacattgcgt gatgatccga tcatgcggtt atgctataac accaccctat
    20161 atatgacccg aactgggttg aagcaccaat caaacggaac aggatgcaaa aatggttact
    20221 ttccacacca atcacggcga tattgtcatc aaaacttttg acgataaagc acctgaaaca
    20281 gttaaaaact tcctggacta ctgccgcgaa ggtttttaca acaacaccat tttccaccgt
    20341 gttatcaacg gctttatgat tcagggcggc ggttttgaac cgggcatgaa acaaaaagcc
    20401 accaaagaac cgatcaaaaa cgaagccaac aacggcctga aaaatacccg tggtacgctg
    20461 gcaatggcac gtactcaggc tccgcactct gcaactgcac agttcttcat caacgtggtt
    20521 gataacgact tcctgaactt ctctggcgaa agcctgcaag gttggggcta ctgcgtgttt
    20581 gctgaagtgg ttgacggcat ggacgtggta gacaaaatca aaggtgttgc aaccggtcgt
    20641 agcggtatgc accaggacgt gccaaaagaa gacgttatca ttgaaagcgt gaccgttagc
    20701 gagtaatcgt ggcgacactc tttattgcag atcttcatct ctgcgtggaa gaaccggcga
    20761 tcaccgccgg ttttctgcgt tttttagcgg gggaagcccg caaggccgac gcgctgtata
    20821 ttcttggcga tctgtttgaa gcatggattg gcgacgacga tcccaaccca ctccatcgcc
    20881 agatggcggc agcgatcaaa gcggtgtccg attccggcgt tccctgttat ttcattcatg
    20941 gcaaccgtga ttttctgctc ggcaaacgct ttgcccgtga aagcggcatg acattattgc
    21001 cggaagaaaa ggtgctcgaa ctttatggtc gccgggtgtt gattatgcat ggcgacacgc
    21061 tgtgcaccga tgacgcgggt tatcaggctt ttcgcgccaa agtccacaaa ccctggctgc
    21121 aaatgctatt cctcgccctg ccgttgtttg tgcgcaaacg cattgccgcg cgaatgcgag
    21181 cgaacagtaa agaagccaac agcagtaaat cgctggcgat catggacgtt aaccaaaacg
    21241 cggtagtcag tgcgatggaa aaacatcagg tgcaatggct gatccacgga catacccatc
    21301 gcccggcggt acatgaactt atcgccaacc agcaacctgc ttttcgcgtg gtactcggtg
    21361 cctggcatac ggaaggttcg atggtgaaag tcacggcgga tgacgttgag ctgattcatt
    21421 ttccgtttta aaaaacccgc aactttgctg atttcacagc cacgcaaccg ttttccttgc
    21481 tctctttccg tgctattctc tgtgccctct aaagccgaga gttgtgcacc acaggagttt
    21541 taagacgcat gtcttcccgc aataatccgg cgcgtgtcgc catcgtgatg gggtccaaaa
    21601 gcgactgggc taccatgcag ttcgccgccg aaatcttcga aatcctgaat gtcccgtacc
    21661 acgttgaagt ggtttctgct caccgcaccc ccgataaact gttcagcttc gccgaaagcg
    21721 ccgaagagaa cggttatcag gtgattattg cgggcgcagg tggcgcagcg cacctgccag
    21781 gcatgattgc cgccaaaacg ctggtgccgg tgctgggcgt gccagtacag agcgccgcac
    21841 tgagcggtgt cgatagcctc tactccatcg tacaaatgcc gcgcggcatt ccggtgggta
    21901 cgctggcgat tggtaaagct ggcgcggcaa acgcggcatt actggcagca caaattctcg
    21961 cgacttacga taaagagcta caccagcgtc tgaatgactg gcgcaaagcc cagaccgacg
    22021 aagtgctgga aaacccggac ccgcgaggtg cggcatgaaa caggtttgcg tcctcggtaa
    22081 cgggcagtta ggccgtatgc tgcgtcaggc aggcgaaccg ttaggcattg ctgtctggcc
    22141 ggtcgggctg gacgctgaac cggcggcggt gccttttcaa caaagcgtga ttaccgctga
    22201 gatcgaacgc tggccggaaa ccgcattaac ccgcgagctg gcgcgtcatc cggcctttgt
    22261 gaaccgcgat gtgttcccga ttattgctga ccgtctgact cagaagcagc ttttcgataa
    22321 gctccacctg ccgaccgcgc cgtggcagtt acttgccgat cgcagcgagt ggcctgcggt
    22381 gtttgatcgt ttaggtgaac tggcgattgt taagcgtcgc actggtggct atgacggtcg
    22441 cggtcaatgg cgtttacgcg ccaatgaaac cgaacagtta ccggcagagt gttacggcga
    22501 atgtattgtc gagcagggca ttaacttctc tggtgaagtg tcgctggttg gcgcgcgcgg
    22561 ctttgatggc agcaccgtgt tttatccgct gacgcataac ctgcatcagg acggtatttt
    22621 gcgcaccagc gtcgcttttc cgcaggccaa cgcacagcag caggcgcaag ccgaagagat
    22681 gctgtcggcg attatgcagg agctgggcta tgtgggcgtg atggcgatgg agtgttttgt
    22741 caccccgcaa ggtctgctga tcaacgaact ggctccgcgt gtgcataaca gcggtcactg
    22801 gacacaaaac ggtgccagca tcagccagtt tgagctgcat ctgcgggcga ttaccgatct
    22861 gccgttaccg caaccagtag tgaataatcc gtcggtgatg atcaatctga ttggtagcga
    22921 tgcgaattat gactggctga aattgccgct ggtgcatctg cactggtacg acaaagaagt
    22981 ccgtccgggg cgtaaagtgg ggcatctgaa tttgaccgac agcgacacat cgcgtctgac
    23041 tgcgacgctg gaagccttaa tcccgctgct gccgccagag tatgccagcg gcgtgatttg
    23101 ggcgcagagt aagttcagtt aagcaggtgc tcatttgatt gccggatgcg gcgtaaacgc
    23161 cttatccggc ttaccgctcc ggtacccatt gtaggcctga taagacgcgt caagcgtcgc
    23221 atcaggcatg gtgcaccgat tgccggatgc tctcatcact acagcgaaat acaggtcccc
    23281 gcttcgcccg ccagcgtctc ttcaattcgc gataacgccc caatccacgc gggtttacta
    23341 cggcttctga cataaccact caccgccgtt accttcggcc ccatcgaacc atcggctttg
    23401 gcaaatggcg ctaactcatc cggtgtggca tggcgaatgg cacgttgctg cggcgttccc
    23461 cagttttcat ataccgcatc agcatcggtg aggatcacca gtccatcggc attaatctgc
    23521 tcggcgagca acgcagcggc gagatcttta tcaatcaccg cttcactccc tgccccatct
    23581 tccgtcacag gcacaccgcc gccgccactg caaatcacca catgcccctc tttgagcaac
    23641 aactcgatgg cttcgctgtc gagaatttta cgcggttgcg gagacgccac cacccggcgc
    23701 aagtatttac catcacgttt catctgccag ccgtaagctg cttccagtgc ctcttgttct
    23761 tctggctgat aaaccggacc aataaatttc tctggctgca aaaacgccgg atcatcaggc
    23821 gaaacctcaa tgcgcgtcag caccgtcgta acaggcggca tctgcggctg tgcgctcaag
    23881 ctctgcgcca gcatatagcc aatcatcccc tggctttccg ccaccagcac atccagcgga
    23941 tacggttcta cctctttcca cgccagattc tgtaacgcca gcagccccac ctgcggcccg
    24001 ttgccgtgaa caatcgccag ccgataagaa cgggccaggc gtgccagcgc gggtacagca
    24061 ctggcgatat tgcgatattg attttccgcc gtcagcgcct caccgcgctg gagtaaggcg
    24121 ttgcccccaa gagctacaac cagtgttttc atggttttcc ttgtaataat tgttggccaa
    24181 gccagaaccc cagcagcgta tctgcccctg acgtatgccc cagcgccagc agcgaatcaa
    24241 tcgcaacagc ggtacgtttc gggcaactca gagcatgaac aaagtgcagg agtggcgagg
    24301 cgaaatatcc ttgcgcggca taacgtaaat aactgacgct caccgctgtg gtaacgagtt
    24361 gaagattgtc ggaacaggca aaaaacgggc gaccagagcg cgcatctaaa gcgccataat
    24421 accaggccgc cagcagcatt ccgctcaacg tgtcatcatg acttggcgtt aatccgggtc
    24481 ctttacccag ccagtgccgc cagtcggtct taacgccatt gagcgcggcc tgaaaacagt
    24541 gacgaaactg gcgtaattca gcggtcagcg gatcgcttgc cgccaacgcc agtggcccga
    24601 aaagcccggt ttcctccgcg cgttgcatcc atgcagctgc aagcggttga ggatgcgcag
    24661 gcggcgtaat acgcagcaaa caataacgct gtggctgttt aaccgtgaaa cgcccgaggc
    24721 gaatcccttg agccacaacc tgtggtcgtt cattgccgca taatccaccg cataacgcat
    24781 cgaattgcgc gcggcgaagc acccatcctc cggggccgaa accactaccc tgacgatgca
    24841 gcgttaacag ttcgccgctt tccgtcatca ggttaatcgc ccgccgccac actcccgcta
    24901 accgccagga ctggcgataa ttcggtgcac tacggctggc aagcagagga tggatgatcg
    24961 tcattgcgcg ttcctcaacc aatccccatg ctttcagcca gtgcttccag cgcctgttca
    25021 aaacacgcca gcggtgcccg cacagtgcct gcgccaatct gcccgatccc cgcctcttta
    25081 tgggcgatac cggtattgat gagtggcgta atgccggttt ctaccacgcg acgaatgtcc
    25141 agtcccaggc acgcgccctg aaaatcccag cctgggatct gcaactgcat attgcgctca
    25201 aggtaaattt ccgccatctc ttcagatacc gctctggccg cttccatgcc acccgcaccg
    25261 acaaagcgcg ttacgccagg cgctgcgatc attgccgcac ctccgatacc aaaggtttcg
    25321 gtaatcgcgc tatcgcccat atccgggttc gcctgctcct gcgagaagcc ggtgaaaaac
    25381 agaccttgcg gagtgtttac aggcgcagta aaccagcgtt cgcccagccc gcttacccga
    25441 atcccgaaca tattgccgtt gcgggtcatt gccgtgacga tgctgcctgc gcggatcatc
    25501 gcgccagcat ccatcgccgc cttgcagtaa gccatcgcga ggttgaggaa gaactgatcg
    25561 gtcacgctga ggaaatccat cacttcggcg atgtgctgtt tatcatgatc gaggcgagca
    25621 atttgtgggg ccagcgcacg cattaacagt gcagaggaag caatattgcg ttgatggaac
    25681 tcatcgccca tcgtaatgcc ctgcgccatc atcgccgtga gatcgatacc gcgctccatg
    25741 cgccccagcg ccgcgcttaa tactggcatt aacacatcgc gcatccagcg atggcgagtc
    25801 aggacatctt cgccgtaagc gccaaaacgc atcactttgc cgataccttc gttgaggttg
    25861 cagtacgccc ggttgccgtc ggtcacgttc tcgaccacca gcatcggcat actggcagaa
    25921 gtaataccgc ccattggacc gacggcattc acatggtgac aaggaatgaa gttcacttcc
    25981 ccctgctcca gtattgccag cgcctgcgct tcatctttcg cccagccttc gaacagacat
    26041 gcgcccacgc acgccccttt catgggtccg gtcatctcct gccagcgcat tggcggcccg
    26101 gcgtgaagca gtgttttgcc ctcgtttagt tcgctgataa gtgaagaagc cggttgcaca
    26161 tccagccagt gtggacgagc gcgacgaatt tgttcgataa ccgcagcatt ggcttgcgcc
    26221 actgatgtaa acatgggaac cccttattgc aaacgttcta ataaacgagc cagtttttta
    26281 ttgccgccgg cgactggcga ccattggtaa tgcacaaccg gtttgctggc gctttgtagc
    26341 tccagcgcaa agctgcgtaa tccgatgtta atcacggcga cgttttccag taatgacggt
    26401 gtgtgttgct gtgtggcaga cgagagcgga tgaattaacg ctgccgccag caaggtggct
    26461 tccggtagcg aactcactac cgcgatcccc gcatcttcca gcgtggcgat ttgctgcgag
    26521 cggcattgcg ggtcacgttc ggtacctgtc accgtggcaa tggcgtacag cggttggctg
    26581 tctgaacgcg cggcacaggc tttttgccag gcgctcacca gcgaggcagc aggatcggcg
    26641 gtcgcaccga agccaatcac gacatcaagc agcaacacgc gcacttgcgg tttagcgccg
    26701 agatcggcaa ttaactggtt gcgtaaggct gggtcgatca tcggatgggg acgcccgacg
    26761 gtgtagaaat catcgcccag atcaatgatc tggtgaccat cggcgtccag catcatgccg
    26821 tgatgatggg tatcgtcggc ttccacgcca aggtgcccgg caagtaatcc cgccgcttcg
    26881 gcagcaagcg taccgccggt atacaaaccg caaataaatc cgctgctgac aggcgctatt
    26941 gcgttacgtc gcgccgtgac gcgtgaaagc aggcaagcca ggcgtgcggc ctcatccagc
    27001 gaggaggcaa accagacatt ctcgtcgcgg gccaccgccg gggtataacc taaaaacagc
    27061 gccaccgtcg gtttgccggt tgctttcatg gcattaacaa ttttcagacg cacagcttcg
    27121 gcaggtggtt ttgaaacaaa tgccagcact tcgcttttct cgtctgcact gagcatttcc
    27181 agcgctgtta gcgcactgat gccgcccact tcacggctga ggtcgcgccc gccaaggcca
    27241 atcgcgtgag taattccctc ccctgccagc gcaatctgcg aacacagctc ctgaatcccg
    27301 gtaccggaag cgccaatgac gccaatattg ccttctggca tcacgttagc aaaagccagc
    27361 ggtgtgccgg caatcatcga cgtaccgcag tccggcccca tcaccagcaa gcctttttcc
    27421 cgcgcgcggg ttttaagttg gatttcatct tccagcgtga cgttatcgga gaacatcatc
    27481 acgttgaggt tgcgatccag cgcctggttt gtcagctccg ccgcatactc gccagccact
    27541 gaaatcagcg ccagattggc atcgggtaat ttctgacagg cgctgtccca gcgacgcacc
    27601 tgcgtcaacg cctggctgct gcctgacccc tgcgccagtt gttttagcgc ctcttcaagc
    27661 tgctgcataa tcgcctgcgc gatccccgca tccgccgctt cgctacgaat tgccacgcaa
    27721 atatcgttcg gcgtggcgtt attaaaatcg tcatgccaga aacctgtggt atctaataac
    27781 gctttattgg cgggcgtacc catcattacg gaaacatcat caacattttc tgattcgctg
    27841 agttttcgtg aaataatcat taaactgacc gaatcctgaa aacacccttt tttaataaag
    27901 gcgtggatca taacaactcc ttaaaaattg cacccatggc aattgcacga gcagtgttaa
    27961 tgtggcgatt acggtatcga tgacaacgag taaataaggt gatgaatatc acacagggat
    28021 tattaacaaa agaattcaaa taaccgccag gctattacgc attatttttt aataagcgtg
    28081 gttgttttaa aaatcaggtt aaaagcgcga agattatcaa ataaattcta actatatttt
    28141 tttgcctgtc tggatcacat aatccagata ttttccctgt atgttaattg cagtcatgct
    28201 tcacaccgtc gttaaaaagg aagacagatg aaaatcagtc gggaaacact ccaccagcta
    28261 attgagaata aactctgcca ggctgggtta aaacgtgagc acgctgcaac cgtggctgaa
    28321 gtattggttt acgccgatgc cagagggatc cactctcatg gcgcggtgcg cgtggaatac
    28381 tacgcggaac gcatttcaaa aggcggcacc aaccgcgaac cggagtttcg tcttgaggaa
    28441 accgggccgt gctcggcaat tttacatgcc gacaatgccg ccggacaggt cgcggcgaaa
    28501 atgggtatgg aacatgccat caaaaccgcc cagcaaaatg gcgttgcggt ggtcggtatc
    28561 agccggatgg gtcacagcgg cgcaatctct tattttgtgc agcaggcagc ccgcgccgga
    28621 ttaattggta tttcgatgtg ccagtccgat ccaatggtgg tgccgtttgg cggcgcggaa
    28681 atttactacg gtactaaccc actggccttt gccgcgccgg gagaaggcga cgagatcctt
    28741 acctttgata tggcgactac cgtacaggca tggggaaaag tgctccacgc ccgctcgcgt
    28801 aatatgtcta tcccggatac ctgggcggtc gataaaaacg gtgcaccaac aaccgatccg
    28861 ttcgcggtac atgctctgct ccccgccgcc gggccaaaag ggtatggcct gatgatgatg
    28921 attgacgtcc tctcaggcgt cttactcggc ttaccgttcg ggcgacaggt tagttcgatg
    28981 tatgacgatt tacacgccgg gcgtaatttg gggcaattac atatcgttat taacccgaac
    29041 tttttcttct ccagcaaatt atttcgtcaa catcttagcc agaccatgcg cgaattaaat
    29101 gccattaccc ccgcgcccgg ttttaatcag gtttattatc ccggacagga tcaggatatt
    29161 aaacaacgca aagccgccgt cgaaggcatc gaaattgttg atgatattta ccagtattta
    29221 atttccgacg cgctttataa cacgtcatac gaaacgaaaa atccctttgc gcaataatta
    29281 ttgagacagg actttcttat gattacacat tttcgtcaag ccatagaaga aacgctgccc
    29341 tggctttcct cttttggcgc tgacccaacg ggtggtatga cccgtttact ttattcgccg
    29401 gaatggctgg aaactcagca gcaatttaaa aaaagaatgg cagcaagcgg gctggaaaca
    29461 cgtttcgatg aagtggggaa tttatatggt cgcctgagtg gcaccgaata tccacaagaa
    29521 gtggttctga gcggttcgca tatcgatacc gtggttaacg gcggtaacct cgacgggcaa
    29581 ttcggcgcgc tggcggcgtg gctggcaatt gactggctga aaacgcaata cggcgcgccg
    29641 ctacgtacgg tcgaagtggt ggcgatggca gaagaagaag gcagccgctt cccgtatgtc
    29701 ttctggggca gtaaaaatat ctttgggctg gcgaatcctg acgacgtgcg gaatatctgt
    29761 gatgccaaag gaaatagttt tgtcgatgcg atgaaggctt gcggatttac tcttccgaac
    29821 gccccactaa ctccgcgtca ggatattaaa gcctttgttg aactgcatat tgaacagggc
    29881 tgtgtgctgg aaagtaatgg gcaatcaatt ggcgtggtga atgcaattgt cgggcagcgt
    29941 cgttatacgg taacgctgaa cggcgaatca aaccatgcag gcaccacgcc gatgggttat
    30001 cgtcgtgata cagtttacgc tttcagtcgc atttgccatc agtcggtcga aaaagcgaaa
    30061 aggatgggcg atccgctggt tctgaccttt ggcaaagtag agccgcgccc gaatacggta
    30121 aatgtggtgc cgggtaaaac cacgttcacc attgattgtc gtcataccga cgctgccgtg
    30181 ctgcgcgatt tcacccaaca gttagaaaac gacatgcggg cgatttgcga tgaaatggac
    30241 attggtattg atatcgattt atggatggac gaagaacccg tgccgatgaa taaggagctg
    30301 gtcgccaccc tgacagaatt gtgtgaaaga gaaaaactga attaccgggt gatgcacagt
    30361 ggtgccgggc acgacgcgca aattttcgcg cctcgcgtac caacctgcat gattttcatt
    30421 cccagcatca acgggatcag ccataacccg gcggaacgca ccaatattac cgaccttgcc
    30481 gaaggggtca aaacgttggc actcatgctt tatcaacttg cctggcagaa ataaggagtc
    30541 ataaatggga tatttaaata acgtcaccgg ttaccgcgaa gatttactgg ctaaccgtgc
    30601 gattgttaaa cacggtaatt tcgcactgtt aaccccggat ggtctggtaa aaaatattat
    30661 tccgggcttt gaaaattgtg acgcgacaat cctctccacg ccaaagctgg gtgcctcttt
    30721 tgttgattat ctggtcacac tgcatcaaaa cggtggcaac caacagggct tcggtggcga
    30781 aggcattgaa acgttcctgt atgtgatctc tggaaatatc actgccaaag ccgaaggcaa
    30841 aacatttgcc ttaagcgaag gtggctatct ttattgccca ccaggctcct taatgacgtt
    30901 tgttaacgcc caggccgaag acagccaaat ctttttatat aagcgtcgct atgttccggt
    30961 agaaggccat gcaccgtggc tggtttctgg caatgccagc gaactggaac gcattcacta
    31021 tgaaggtatg gatgatgtga tcctgctgga ttttctgccc aaagagttag gttttgatat
    31081 gaacatgcat atcctctctt ttgcaccagg tgccagccac ggttatatcg aaacacatgt
    31141 tcaggaacac ggtgcctata ttctttccgg tcagggggtt tataacctcg acaataactg
    31201 gatcccggtg aaaaaaggcg attacatctt tatgggcgct tattctttac aggctggtta
    31261 tggtgtaggg cgtggtgaag cgttcagcta tatttactcg aaagattgca accgcgacgt
    31321 agagatttaa gtcataccac tcgcctgatt atttttagct atgttggatt ttgccacggt
    31381 ttataccgtg gctttttttg taacattgcc tgatgcgctt cgcttatcag gcctacatga
    31441 tctctgcaat atatagaatt tgcgcgattt tcaggaagga tatagtactc atgccgcatc
    31501 cggcatgagt actgcactct tatgcgatat aaatcgcatc cgctttaaag gtaagggtca
    31561 gtttttaatt ccctgaccta ttttaatggc gcaggcaata tttcgtgcgc tgttgaagag
    31621 attggtttca ccgctggcga gtacttcggc taaaggtgcc agacgtggca aaatgctgaa
    31681 taccgcatca atgccgtact ggtgcaccac ttccacgcca tcacccaata cgccagcaat
    31741 cccaatcacc ggtacattaa actgcttcgc caccgacgcc acacccagcg gcgctttacc
    31801 gcctgccgtt tgcgagtcga tgcgcccttc cccggtgatc accagtgctg caccctgcac
    31861 tgcctgcgca agattgaccg cattcaacac aatctcaata cccggtttaa tatccgcatt
    31921 gagaaatacc gccgccgcaa tacccatacc gcccgcagcg ccgccgcccg ccatctggca
    31981 gacattaatt tccgtttgct gttgtaaaac acgggcgtaa ttttgcagcc cctgttccag
    32041 ttcttcgacc atttccggcg ttgccccttt ttgcgggcca aacaccgccg ccgcgccgcg
    32101 tgcccctacc agcgggttat caacgtcaca tgctacttca atatggcaat tcgccagacg
    32161 cggatcgcat tcatccatct caatgcttgc cacgcgcgct aaattaccgc cgttagccac
    32221 cagcacctga ccgtcggcat caaggaaacg cacaccgagc gcctgcgcca tacccatacc
    32281 accgtcgacc gtcgcactgc cgccaatgcc gccaatgccg agaataatat ggcgaatgcc
    32341 gttatccagc gcatgacgaa ttaactcccc cgtaccaaaa ctggaggcca gcaacggatt
    32401 acgcttttca ggcgcgacca gcatcaggcc acttgctgcc gccatctcaa tcaccgccgt
    32461 tttcccgtcg ccggtaaggc cataaaaagc attcactttt tcgcccatcg gcccgcagac
    32521 ttcaagcgtc acgatgttgc cgcccgtcgc ggcgaccatc gcatccaccg ttccttcgcc
    32581 gccatccgct atcggcaaac agatatagtt cgcatcggga aagagggtcg aaaacccggc
    32641 tttaattgcc tgacaacatt tttctgcact taagctctct ttaaaagagt ctggcgcaat
    32701 gacaatcttc attatcaatc ctttttcaac aaattattcg atacgttccc acggaataag
    32761 gttatccata agaacagcca gtaaaatccc gaccaataag ccgttactga gcagaggacg
    32821 aagcgttaat ggaaggtctt gcagatacac aggcggtaat gccattaaaa atatgccgac
    32881 aaataacggc aatgcgagtc gataaatatt gcgagcggta aaggttattt gctggctaaa
    32941 caccagcgcg gaaaagagta aaggcagata agaaaccagc atgaccgcgc tactcacggg
    33001 taaagggata ctgcaaaaca gtcgtgtgag tgccggaacc agcgccacca gcaggcaaat
    33061 aacgctgcca taaataaacg aacgccgcgt gtaatcgcct gtctgggtta ataaaccaat
    33121 ggatgaaaca aacggtgaaa atggaattac tgccagcggt acggttagca gtgtcataaa
    33181 tccggtcgcc acaaagctac gacgataacg cgtattcccc gcgccctgct gcggataaaa
    33241 aacatccgtg ccccgaatcg caccgtaggt attgctgata ttcaccagac ctgtaatcac
    33301 cgccgtcaga ataattcccg gagacaaagc acctccactg ccgagcggga accactgcca
    33361 gtgcaactca ccggagagcg agtgcgaaga aggaaagcaa aagtaccaca acaaccagcc
    33421 ggttatggtg ccgaccagca ggccataacg ggcaaaacgt tgcggcagga agataatcat
    33481 ccccagtacc aggcacatca ccgccaccga gagcgcgaac ggcggtaact gaattttaaa
    33541 atttgggtcg gctatgccaa acggcagccc gagcatacct ttgaaaaaga tagtggtcag
    33601 ctgcgcgccc agcatcaaca taaacaagac catcaccgac ggcgtaaata accgtgccag
    33661 gcgatggcct aatccgctaa aaccaatcaa catcgtcagc acgccggaga gcgcaatccc
    33721 caccgccagg ctggtagcga tatcgttgat cggtgtcccg cgcgatgctt cgccaagggt
    33781 gatagtaagg atggttcccc accacaggcc acccggccct tccataatag cgcgacgatg
    33841 accgcagaac gcctgagcga agcaggccag tgcggtagca agaaaagcat attgcgtgag
    33901 cgtaagcagg ctactttgcg gcaactgaaa agcagaaagt agcgtaggag gaaccacaac
    33961 cgtgttgcaa aaaataaaga aaaaccactg aaatcctgat aacaggcttt cgcggctcac
    34021 tgcaaaattg aacataaaca caccttaacc ggagaatgcc cgccgcaagg acgggcattg
    34081 caggggccag attactgctg atgtttaagg ataaattgac ctttcggcgc aacagggaag
    34141 ccctgttcaa tgtcgtaaat cacatcacca cgtaagatgg ttttcgtgat acgcgcgcca
    34201 atggtacggc caacatacgg gctgactttg tggcgatatt ccagatcgtc attggtaaga
    34261 acatagctgc tattcggctg aatgaagacg aagtcggcat cttttcctgg ggcgatacgg
    34321 cctttttgct gcagaccgaa aatatctgct gcgttagtcg ccattaattt gccgaacatt
    34381 ggcagagaca ttccgcgttt ctgtaccgct tcatcgaaca tcacgtccat acagttttgc
    34441 agaccggcaa taccgcccca tgcttccatg atgttgccgg ctttcatttc cggagggcat
    34501 ggtgagtggt cggaaaccag gcagtcgatt tcaccgataa acagtttttc ccacatgcct
    34561 ttctggtttt ccagatcgcg gatcggcggt gaacacttcg ccagagtgcc gatttcttcg
    34621 aactgatcgg tatccagtac aaagtaatgc gggcaggatt cacaagtaac gtcctgacct
    34681 tcctgacgtg cacgagtcac ttcttcaacg ccttccgggc tgctgatatg gcaaacgtgc
    34741 agacggcaac cggcaacttt cgccagatac agcacgcggc gaatcgcttc cacttcggta
    34801 aataccggac gcgaagccac atagtcatgg gcagttacgc gaccttcacg tttcgcttct
    34861 tcgcccagtg cgtcacagat cagcgcgttt tcgcagtgca ccagcaccgg ctgccccagt
    34921 tcgcccagct tctgcgcacc tttgaagaac tgccagtcgt ttacatcacg gaagtcgttg
    34981 tcgataccgc gatcgccaca ggtcgcaacg aagcatttga agccgacaac gcccacttca
    35041 tccagctcat gcagacggtc gatgttgtaa gacaccaggc caccgagttg tgccgcatcg
    35101 atagtcagct tgcctttagc ggcatcgaac ttcagttcaa ttgaagcgcg gtcaaccgtt
    35161 gcaggcagct ggttgagcgg catttcgatc atggtggtga taccaccttt tgccgctgcg
    35221 cgagtaccgg tttcataacc ttcccagtgg ctacgacccg gttcagaaat atgggtgtac
    35281 gcatcaacca tgcccggcga aaccaccaga ccagacgcat ccataacttc ttttgcatcg
    35341 cccagatcct gaccgatagc agcaattttc ccgcctttaa cggcgatatc tacaacgcga
    35401 gcttcgtttt ctaaaataac ggtgccgttt ttaatgatta aatcaaaaga cataacaaac
    35461 tccttgtaaa atacagattg ttttttaact caatgttcaa tatcgggatt aattaaccta
    35521 tggttttttg ctctcctgtt ttttctgctg ttgtacgctt ctttaataag gcgtaggccg
    35581 caaaggcgac gatgacgccg acaaaccatg aaacacgcga taacggttcc ataaagggaa
    35641 taaacttacc gccaagagat aaaataacgg ccaccagagt tactgaaaac gcagtgaggt
    35701 taaaaccgtt atcgtaatat ttataatcgc caggtgcggt atacagttca tcaagattaa
    35761 ttttcccacg catcaccaca aaataatgcg ccatcatgac accaattacc ggaccaagca
    35821 ttccgccgat aatatcgagg aacagataaa tactgtcctg attttccatt aatttccacg
    35881 ggcagatcag caggctgata atactggcaa tcagtacgcc gtttttatag ttcagtttcg
    35941 tcggtgcaat agcggcaatc tgataaccgg caggaataat attaccggtg gcgttagtgg
    36001 agatagtcgt catcagaata accagtaccg caaagaacga ggcgaacaag ctgtcccaac
    36061 gctgaacaat atccagcacg ttccaggtat cagcgccgta atgaatactg gctccggcaa
    36121 taatacatac ccccgcgacc gcaaacagaa tataggccac aactaaaccc agcgtttgcc
    36181 ccagcgcttg ctcacgaaac gagtgggcgt tttgcgtaaa gtcggatgcg ctcaccgccg
    36241 gtgccgccca gaccgcaact accgcgttta tcaccaccag gaacaggaag ccactgtttt
    36301 ctgctttctg aataccgctc ggaatgtagt caaagattgg accgagcccg accagcgaaa
    36361 tcgcccaaat cgccataccg ccgaaaacga tatagatgca cgggttaaga atggcagtga
    36421 atttatttaa aactttgcca ccgccaaaac ctataccaac gttgaccagc cagaagagta
    36481 agaaagtaat taagcccggt agagaaagcc ctaacagcgt gaaatcacca ccgagagtta
    36541 aaaatcccgg ccagattttg ccaatcagaa tcaagcaggc cagtgacccc gcgtaacatt
    36601 gcaggccaaa ccacataatg gcagcaatac cgcctcttaa taatccggga aacagtgcgc
    36661 cacgtacgcc gtaagaagca cgcaggatca tggcaaacgg tacgccgtat ttactgcccg
    36721 cagcgccgtt taataccatt accgcggcaa tgaaaaaggc gctgaggata attgccagca
    36781 taatactaaa ggtagacaag ccgagaataa aaaagccgcc gaccatcaca taattgggaa
    36841 cgttatgaac cgaacccatc cataaggtaa aatagttaaa tgttttccag gtccgctggc
    36901 tttgcgtttt cggcaataga tcttcgctat agccgcgttg ctggaatagt tttctctgat
    36961 gttccataaa tccctcttaa tttcacgtat cgaataacgt tgttttaatc tagctatgag
    37021 gaacaaataa aataaaaata ttatttttaa tactgaggca acatctttgg ctgctttctt
    37081 ttttaatatt gctggccgat tcattttctg atatcggcca tttttattgc gggtatcagg
    37141 ccagtttatg gttagccatt aattccagcg cctgcactaa cgcagagtga tccaactggc
    37201 tgccaccgtt tgccgcacag gtattaaata actcctggca ggtcgcagtg tttggcaggt
    37261 tcagcgcaag tgctttcgca ctttgcagtg ccaggttgag atctttctgg tgcagagcga
    37321 ttttgaagcc cggattaaag gtgcgtttaa tcatacgctc gccatgaact tccagaatac
    37381 gtgaggaagc aaagccgccc atcagcgcct ggcgcacacg taccgggtcc gcaccggctt
    37441 ttgaagcaaa tagcagggct tcagaaaccg cttcaatatt gagcgccacg ataatctgat
    37501 ttgccacttt gcaggtttga ccgtcgccgt taccgcccac gagggtgata tttttaccga
    37561 gcagttcaaa cagcggttta acacgttcaa ataccgcttc atcaccgcca accataatcg
    37621 acaacgtccc ttcacgcgca ccgatttcac cgccggagac tggcgcatcg agataatcgc
    37681 cgcccagttc attcacctga cgagcgaaac gcttagtttc aatcggggaa atggagctca
    37741 tatcaacaat ggttttgccc ttcagcgagg ctttggtaca accattttca ccgaacagaa
    37801 cttcttcaac ctgtggtgta tctggcacca taataaaaat gatgtccgat gcttccgtta
    37861 cctggcgcgc agtttcaaca ctgacggcac ccagtgacag taattcatca gcaaccggac
    37921 caatggttgt gacatgcagt tgatgaccgg cacgcgccag attaatggcc atcggtgtac
    37981 ccataatgcc taagccaata aatcccagtt tcataattaa cctcttttaa atttcgcttt
    38041 tcctgaattc agacaacacg atcccctgcc aacttagcgg cctaaaaaag cattgcctga
    38101 acagcgttac gttttaacgg tacggatcca tccagcgtaa accggcttcc gtggtggttt
    38161 ggggtttata ttcacaccca acccaaccgt tgtagtcaga attttcgatt accttaaaga
    38221 gataatcata attaatttct ccggtccccg gttcgccgcg atgcggatta tcagcaattt
    38281 gcaggtggcc aattttatcg gcccactgag tcatggtgtt ggttaattca ccttccatcc
    38341 gctgcatgtg ataaatgtca tactgaattt tcaaattgca gcaaccaaca tcatcaatca
    38401 atttcagcgc ctgccgagtt ccggtgagat ggaaaccagg aatatcaaaa tggttaatag
    38461 gttcaatcag taataaaata tcttctttca tcagcatatt cgcggcataa cgtaggtttt
    38521 ctacaagcgt tgcgtgaatc tgttcactgc tgaaaccagc cggcgtttta ccgaccagac
    38581 agttaatttt tttattcccc agcgcacggg cataacgaat cgctgctgct acgccatccc
    38641 gaaactcttc ttcacggcca ggaatacagg caataccgcg ctcccccgcc gcccagtcac
    38701 ccgccggtag attgtgcagc gtatgttcga gtttattact cgccagcacc tgttttaatt
    38761 cttcaatgtc gtagtcataa ggaaacataa attcaacgcc gcgaaaacca cactgcgcag
    38821 ctttctcaaa acgggcgaga aaatcatatt ctccaaataa catcgataaa ttagcagaga
    38881 aacgtaacat tatttatctc ccttattcat agtgcatgaa gcaggtttca gtcggtgcgt
    38941 ccgctgcgtt atcggcgata tcttcaaatt ccatgacgtt atgcagttcg ctgcccatcg
    39001 aaatattggt cacacgctcg agaataactt ccacgactac cggtacccga tattgcgcca
    39061 ttaaggcttt cgcctgttca aaggctggcg caatatcttc cggtttgaag acgcgaatcg
    39121 ctttacaacc taaaccttcc gctactttta cgtggtcgac gccgtaaccg ttcacttcgc
    39181 tggagttgat attctcgaaa gcgagttgca cgcagtagtc catatcaaac gcgcgctgcg
    39241 actgacgaat cagccccaga taagcgttgt tgaccagcac atggatgtac ggaatgttga
    39301 actgcgcgcc aacagctaac tcttcaatca ggaactggaa gtcaaagtcg ccagaaatcg
    39361 ccaccacatt gcgtttcgga tcagcggcac aaacccctag cgcagccgga atcgtccagc
    39421 ctaacggacc agcctgacca cagttgatcc agtggcggtc tttaaagaca tgcagcattt
    39481 gcgcagcggc aatttgcgac agaccaatgg tggtgacata gcaaacatcg cgaccaaagg
    39541 ctttgttcat ctcttcatac acgcgctgcg gtttcaccgg cacgttgtcg aaatgggttt
    39601 tgcgcagcaa agtgcgtttg cgctgctggc agtcggcgac ccattcttta cgacacggca
    39661 gacgacccgc tttttgcatc tcctgcgcca cttcaaccag cagtgtcagc gccgctttag
    39721 catcagagac aatgccgaga tccggacaca gcacgcgacc aatttgcgtc ggctcaatat
    39781 cgatatgaac gattttgcgc ccttcggtgt atttctctac cgaaccggta tgacggttag
    39841 caaaacggtt accgatacca aacaccatgt cggacgccag caacgttgcg ttaccgtaac
    39901 gatgcgcggt ttgcagaccc accatcccgg ccatcagttc atgatcgtcc gggatacagc
    39961 cccagcccat cagcgttggg atcaccggaa cgctggtcag ttcagcaaac tgttgtaaca
    40021 gtgcagctgc gtcagcatta attaccccgc ccccggcaac aatcaccgga cgttcggcct
    40081 ggattaacat ttctacagct ttttcgatct gcatacggct ggcagcaggt ttgtagaccg
    40141 gcagcggttc gtacatgtca ggatcaaact cgatttccgc aacctgaacg tcgaacggta
    40201 aatccaccag taccggaccc ggacgaccag aacgcatcag gtgaaatgcc tgttgcagca
    40261 cgcgaggcac cagcgccgct tcacgaactg taaccgccat tttgctgacc ggtttagcaa
    40321 ttgcttcaat atctacggcc tgaaaatctt ctttatgcag acgggcgcgc ggtgcctggc
    40381 cggtaatgca cagaatagga atggaatcag cagaagcgga atagagcgcg gtgatcatgt
    40441 ccgtgcccgc aggaccggaa gtccccagac atacgccgat atttcctgcc gttgcgcggg
    40501 tataaccttc cgccatgtgc gaagcacctt ccacatgacg cgccagaatg tgacgaatac
    40561 cgccgtgctt acgcatcgct gagtagaacg gattgattgc agctcccgga acaccgaagg
    40621 cggtggtgat accttctttc tccagcacat acattgccgc gtcaacggct ctcatttttg
    40681 ccattttatt cctaccttta tttattggaa aaattttcca actttcaaat tttgtcaaaa
    40741 aaacccgctc cgcagagcgg gatgcgacag acattatgga tgtgctttca gtcccaacgc
    40801 cgtgctgata tcgcgggcgg tgtctctgac cagctcaccc tgactgacaa aacgatcttc
    40861 tgtcagtctt gatgaaggcc cggagatgga gatagcggca acaacactac ctacatcatc
    40921 gtaaattgct gaagctaagc aattcagacc tacaacatgc tcttctttat ctacggtata
    40981 gcccagttca cgcgcttgtt ccaggtcctt cagcaaggtg ggcatatcca caagcgtagt
    41041 tggcgtaaac tgctgcaaac cggtttgcag aatgatgctc atcaactcct cttccgccag
    41101 cggataaagc agcgctttgc ccgcaccgga agcatgcagc ggcagacgac tgcccagtgg
    41161 cgcacacatc ctgaccatcg atttacactc taactgacca attaataccg cttcattgcc
    41221 gttacggatc gcgacattga ccgtttcgcc ggaaagtaac atcaggcggc gcataaacgg
    41281 cccggcgacg gagaggacat cgcggttatg gatgtacgct gcaccgacgt taaagacacc
    41341 taatcctata tgccaccagc ctaattgact gtcctgatag acaaaatccg ctgcctgtaa
    41401 aaccttcagc aagcgaaagg tcgtggagag cggcaaatcc agattgagag aaatatcgct
    41461 aaccgacgaa cttcccccac ttttttccaa atattgcaga atcgcaattc cccgctctaa
    41521 cgcctgtgcg cccttctgtg ccacaggctc cgcctgtcct ggcctgccgc gccgtctaac
    41581 ttccgtcata cctacgttaa ctccgaccta caagtgagca taaacaatga acaattagtt
    41641 tatcttatgc aagtcggttg caggcgtcac gcaaaacaga gttcctgttc aggaatactt
    41701 tcaacatcac agttgtcact gccgccgcga tcgatggtca gaaaatcggt gacgcgctgc
    41761 caggcgaaaa gtgggtgatg ccagacgtta cgatggtaat tcactccctg ttcgccgttg
    41821 gtgataaacg cccgcagcgt tgacaggtct ggtttgtcgt cgcctaacgc cacgaccacc
    41881 acaaacactt cacctttcat cgggataaag gcctgagtgc ccagcggatg acgttcgagt
    41941 tcgtgaatgg tcagcggcag attcgccggt tgcgcgcggt taatgctgat aagcgtacgg
    42001 tcttgctcaa gaatctcaac cagcgccaaa tcgtggtaac gctccaccag gccattgtta
    42061 atatggaaaa aatcccgttg ctgcgtttcg attacgtcgc cataagcact aaaggcttcc
    42121 tgacttaacg gtaatacctg aagtttcatc tttttgtcct tgtaacataa ttccgttcaa
    42181 ccagcgccga ccggtcagct tggtttcaat aacaggcaat ctaaaacctt aagagaacaa
    42241 catcaataca aaagttggaa tatttttcca aaactagatt tagtccacaa attgaacagg
    42301 gttatcatgt aaaaatactg ataaatcaga ggataagaaa atcacgcaac ttgcggattt
    42361 aaaaggggat acctgaagat gttcgatcca gaaaccttgc ggactttcat tgcggttgct
    42421 gaaacaggaa gtttttcaaa agcggcagaa cgattatgta aaaccacggc gacgatcagt
    42481 tatcgcatta aacttctgga agagaatacc ggagtagcgc tgtttttccg tacgactcgt
    42541 agcgtaacgt tgacagcggc tggcgagcat ctactttccc aggccagaga ctggctgagc
    42601 tggctggaaa gtatgccaag cgagctgcaa caggtgaatg atggcgtgga acgccaggtg
    42661 aatattgtca tcaacaacct gctctacaac ccccaggccg tcgcccagtt gctggcgtgg
    42721 ctgaatgaac gttacccctt tacccagttt cacatctccc gacaaatcta tatgggcgtc
    42781 tgggattcgc tattgtacga aggtttttca ctggctatcg gcgtcacggg aaccgaggcg
    42841 ctggcaaata cctttagtct tgatccctta ggatcggtgc aatggcgctt tgtcatggcg
    42901 gcggatcatc cgctggcgaa cgttgaagag ccgctaacag aagcgcagtt gcggcgcttt
    42961 ccggcggtca atattgaaga cagcgcccgc accttaacca aacgcgtcgc ctggcgattg
    43021 ccagggcaaa aagagattat tgttcctgat atggaaacga aaatcgccgc ccatcttgcg
    43081 ggcgttggca ttggtttttt gccaaaatcg ctttgccagt caatgatcga taatcaacaa
    43141 ctggttagcc gggtaatccc aacgatgcgc cctccttcgc cattgagtct ggcatggcgc
    43201 aaatttggca gcggcaaagc ggtagaagat attgtgacct tgtttaccca gcgcaggccg
    43261 gaaatcagcg gatttttaga aattttcggc aacccacgca gttaaaaatc aagtttcctt
    43321 gcgcgttggc gcgtaaaata gcgcgcaagc tgatttgagg ttcactttta actatgcaag
    43381 agagacacac ggaacaggac tatcgtgccc tgctgattgc tgatacgccc attattgatg
    43441 ttcgcgcccc tatcgagttt gagcaaggcg caatgcccgc cgctatcaat ctgccgttaa
    43501 tgaataacga tgaacgcgcc gccgttggca cctgctataa acagcaaggc tcagacgcag
    43561 cgctggcgct ggggcataaa ctggtggcgg gtgaaattcg tcagcagcgc atggacgcct
    43621 ggcgggcagc gtgcctgcaa aatccgcaag gtattctctg ctgcgcccgt ggcggtcagc
    43681 gctcacatat tgtgcaaagc tggttgtatg cagcggggat tgattatccg ctggtggaag
    43741 gcggttataa ggcactgcgc cagaccgcga ttcaggcgac tattgaactg gcacaaaaac
    43801 cgatagtgct gattggcggt tgtactggca gcggtaaaac gctgttagtg cagcaacagc
    43861 cgaacggtgt tgatctggaa gggctggcgc gccatcgcgg ttcggcgttt ggtcgcacgt
    43921 tacaaccaca acttagccag gcgagttttg aaaacctgct ggctgccgaa atgctgaaaa
    43981 ccgacgcccg tcaggatttg cgcctgtggg tgctggaaga cgaaagccgg atgatcggtt
    44041 cgaatcacct gccggaatgt ctgcgcgagc gaatgactca ggcggcgatt gcggtggtag
    44101 aagatccgtt tgagatccgt cttgaacgcc tgaacgaaga gtatttcttg cgtatgcatc
    44161 atgattttac ccacgcttat ggcgacgaac agggctggca ggaatattgc gaatacctgc
    44221 atcacggact ttcggcgatt aagcgtcggc tggggttaca gcgctataac gaactcgctg
    44281 cccagctgga tacggcgctg acaacgcaac tcaccaccgg cagcaccgac ggtcatctgg
    44341 cctggctggt gccgttactt aaagaatatt acgacccaat gtatcgctat cagcttgaga
    44401 aaaaagcgga aaaagttgtc tttcgcggtg agtgggcgga agtggcggaa tgggttaagg
    44461 cgcggtaatg agggacgtat ttgtattagt cgcgacttga tctgacacat ggccttgaaa
    44521 ggttgagagt taccggtttt gatatgagtg tcgaatcctt atccaaaaca agaggtaact
    44581 ctcatgcttc atactgccaa tcccgtcatc aaacacaaag ccggtttgct caatctggct
    44641 gaagaactca gcaacgtatc aaaagcctgt aaaatcatgg gcgtttcgcg tgatacgttt
    44701 tatcgttacc gcgaactggt cgctgaaggc ggtgtggatg cgcagattaa tcgtagtcgc
    44761 cgcgctccta accttaagaa ccgtaccgat gaggcaactg aacaggctgt tgttgattac
    44821 gccgtcgctt tcccgacaca tggtcagcac cgggccagca acgagctgcg taaacagggg
    44881 gtttttatct ccgacagtgg tgtccgttct gtctggttac tccataacct tgagaatctc
    44941 aaaagacgat actgaacaaa ttttatcagg tgacgttccg taaaaagttg tatggagttc
    45001 tcgatgcagt cggcgcgact atcagtaccc ccgctgaacc ctggactatc gggccgccgt
    45061 actggggcat gtcaccctgg cgtgccgctg gttttccgct aatggtttat ttcctctttc
    45121 tttcccggca agaatttatt ttaatccgga aagattattt attttcagat agggactcac
    45181 aataaacacg taacatttta attaatgcat ggctgaatat gtattacaca atgtcagata
    45241 tcgtaaaaga ccagtgcaat tctatgtaaa ctctctgact gttcatttta tttgttgttt
    45301 cagggtcgga actcaccctc aataaacaac tgaatacgag tagatattca tcgtctgagc
    45361 tatatggctt tacacaatag ccgacatgtc ttttctcagt caggaatttt acttttcctc
    45421 actacgaagt tgacgacttc gatatgggat agactcttaa ttcaagcaat tactctgcac
    45481 aagcatcagg caatccagct ccaatatttg ttataaaata atgcgtaaca ttaatttcac
    45541 caacaaaaaa aaggtttaca ccatcccctt gccctggtat cttaccatat ttacaccaca
    45601 ctttttttgt atctggatca ttctttgata cgattttcgc cgcttgttcc ctggccctca
    45661 ctgtcaatgt tttcttaaac ctacccccat catcatttgt tagggaaaaa taagaaaaaa
    45721 aataatcatc ttctacattt gtgtatattg atatatccac cgctacgtgc ggaaaaaaat
    45781 ctgggagcca tgtaaaaact ccatcatttt ctggtattgc catatagttt atgtaatgtt
    45841 tacttacaag agattcatct ttgtataaat aaagataagt aattacgcat aaaacaacaa
    45901 tgattataat agcaaaaata aatattatca tctttgatag attacttgag atagccagca
    45961 tcttgtaaag cctttatcgt ttttttatgc tctggattaa tataatcact acatctatct
    46021 gagcaatctg ttgttgatgg acatgtcaac ccatggtcat ttacagccaa gtctttttta
    46081 ttatcttcaa tcatttcaga atgggatagc tttacttttc tgccgtacat accgaacaga
    46141 tttaacccgt aatctctaat ctctttttca taacccagcc ctttcgccga tcggctaaca
    46201 ccagcgtcat ttaatttaga cactcgacaa aatgccatac aatggaaaaa ttgatcagtc
    46261 ccacctaaat ttaatctctt catatcttca taagtatcag ataatatatc ccatgctctt
    46321 tgatggttta gttgatcttt tcttcttatt agcgctacat ctgcgggact taaccctaat
    46381 ggatcaatac cattcaccgg attcagcgga tacgcataca gactccatcc ccccgccagc
    46441 ccaatcgggt cctgggtgat ataccgcccc tgcaacggat cgtagtaccg gtgacggtta
    46501 taatccagcc ccgactcctc atcgtactgc tgccctggca gacggtatgg ctgatacagg
    46561 taatacgggt tctcttcatt cagctggttg ccccattcat cgtattctgc actccacaca
    46621 gtattgccgt cttcgctgat gagcgccagc ggcaggcccc ggtggtcgca gtggtaaaaa
    46681 tgaacttttc gcgccggtgt gtattccggc tccacctgtc tggccagttg ctccaccgtc
    46741 agcccgcact gcgcaagcca cgcccggctt tcgtcactca cgcggtctgc ccggatttct
    46801 tcctccagtc tgtccagcag ccgcaccagt tcagccggga acaccacacc gtgaccgtct
    46861 tcgctccctt cctgctgtaa cttctccgcc aggctgcggc actgcgcttt ctcccgttca
    46921 ccgttgtctg tttcgatgcg gatgagcggc gcgaagctcc ccggctg
//
LOCUS       DAZGXZ010000020        45336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000020 DAZGXZ010000000
VERSION     DAZGXZ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.020"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            317..820
                     /gene="ftnB"
                     /locus_tag="ACHKJF_001277"
     CDS             317..820
                     /gene="ftnB"
                     /locus_tag="ACHKJF_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP0948667.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(899..1149)
                     /gene="yecJ"
                     /locus_tag="ACHKJF_001278"
                     /pseudo
     CDS             complement(899..1149)
                     /gene="yecJ"
                     /locus_tag="ACHKJF_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
     gene            complement(1264..1350)
                     /gene="azuC"
                     /locus_tag="ACHKJF_001279"
     CDS             complement(1264..1350)
                     /gene="azuC"
                     /locus_tag="ACHKJF_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP0948668.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1613..1936
                     /gene="yecR"
                     /locus_tag="ACHKJF_001280"
     CDS             1613..1936
                     /gene="yecR"
                     /locus_tag="ACHKJF_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP0948669.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2107..2604
                     /gene="ftnA"
                     /locus_tag="ACHKJF_001281"
     CDS             2107..2604
                     /gene="ftnA"
                     /locus_tag="ACHKJF_001281"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP0948670.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2642..2881)
                     /gene="yecH"
                     /locus_tag="ACHKJF_001282"
     CDS             complement(2642..2881)
                     /gene="yecH"
                     /locus_tag="ACHKJF_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP0948671.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            3072..4283
                     /gene="tyrP"
                     /locus_tag="ACHKJF_001283"
     CDS             3072..4283
                     /gene="tyrP"
                     /locus_tag="ACHKJF_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP0948672.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4345..5010)
                     /gene="yecA"
                     /locus_tag="ACHKJF_001284"
     CDS             complement(4345..5010)
                     /gene="yecA"
                     /locus_tag="ACHKJF_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP0948673.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5206..5292)
                     /locus_tag="ACHKJF_001285"
     tRNA            complement(5206..5292)
                     /locus_tag="ACHKJF_001285"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5256..5258),aa:Leu,seq:taa)
     gene            complement(5305..5378)
                     /locus_tag="ACHKJF_001286"
     tRNA            complement(5305..5378)
                     /locus_tag="ACHKJF_001286"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5344..5346),aa:Cys,seq:gca)
     gene            complement(5433..5508)
                     /locus_tag="ACHKJF_001287"
     tRNA            complement(5433..5508)
                     /locus_tag="ACHKJF_001287"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5473..5475),aa:Gly,seq:gcc)
     gene            complement(5660..6208)
                     /gene="pgsA"
                     /locus_tag="ACHKJF_001288"
     CDS             complement(5660..6208)
                     /gene="pgsA"
                     /locus_tag="ACHKJF_001288"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP0948674.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6265..8097)
                     /gene="uvrC"
                     /locus_tag="ACHKJF_001289"
     CDS             complement(6265..8097)
                     /gene="uvrC"
                     /locus_tag="ACHKJF_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP0948675.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8094..8750)
                     /gene="uvrY"
                     /locus_tag="ACHKJF_001290"
     CDS             complement(8094..8750)
                     /gene="uvrY"
                     /locus_tag="ACHKJF_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP0948676.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8892..8963
                     /locus_tag="ACHKJF_001291"
                     /pseudo
     CDS             8892..8963
                     /locus_tag="ACHKJF_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            9046..9222
                     /gene="yecU"
                     /locus_tag="ACHKJF_001292"
     CDS             9046..9222
                     /gene="yecU"
                     /locus_tag="ACHKJF_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP0948677.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9209..9433
                     /gene="yecF"
                     /locus_tag="ACHKJF_001293"
     CDS             9209..9433
                     /gene="yecF"
                     /locus_tag="ACHKJF_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP0948678.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9500..10222)
                     /gene="sdiA"
                     /locus_tag="ACHKJF_001294"
     CDS             complement(9500..10222)
                     /gene="sdiA"
                     /locus_tag="ACHKJF_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP0948679.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10452..11204)
                     /gene="tcyN"
                     /locus_tag="ACHKJF_001295"
     CDS             complement(10452..11204)
                     /gene="tcyN"
                     /locus_tag="ACHKJF_001295"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP0948680.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(11201..11869)
                     /gene="tcyL"
                     /locus_tag="ACHKJF_001296"
     CDS             complement(11201..11869)
                     /gene="tcyL"
                     /locus_tag="ACHKJF_001296"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP0948681.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11884..12870)
                     /gene="dcyD"
                     /locus_tag="ACHKJF_001297"
     CDS             complement(11884..12870)
                     /gene="dcyD"
                     /locus_tag="ACHKJF_001297"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP0948682.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12975..13775)
                     /gene="tcyJ"
                     /locus_tag="ACHKJF_001298"
     CDS             complement(12975..13775)
                     /gene="tcyJ"
                     /locus_tag="ACHKJF_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0948683.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13863..14414)
                     /gene="fliZ"
                     /locus_tag="ACHKJF_001299"
     CDS             complement(13863..14414)
                     /gene="fliZ"
                     /locus_tag="ACHKJF_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP0948684.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(14460..15179)
                     /gene="fliA"
                     /locus_tag="ACHKJF_001300"
     CDS             complement(14460..15179)
                     /gene="fliA"
                     /locus_tag="ACHKJF_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP0948685.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15344..16837)
                     /locus_tag="ACHKJF_001301"
     CDS             complement(15344..16837)
                     /locus_tag="ACHKJF_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFP0948686.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            17092..18504
                     /gene="fliD"
                     /locus_tag="ACHKJF_001302"
     CDS             17092..18504
                     /gene="fliD"
                     /locus_tag="ACHKJF_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0948687.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            18519..18929
                     /gene="fliS"
                     /locus_tag="ACHKJF_001303"
     CDS             18519..18929
                     /gene="fliS"
                     /locus_tag="ACHKJF_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0948688.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            18929..19294
                     /gene="fliT"
                     /locus_tag="ACHKJF_001304"
     CDS             18929..19294
                     /gene="fliT"
                     /locus_tag="ACHKJF_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP0948689.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            19372..20859
                     /gene="amyA"
                     /locus_tag="ACHKJF_001305"
     CDS             19372..20859
                     /gene="amyA"
                     /locus_tag="ACHKJF_001305"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0948690.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20893..21306)
                     /gene="yedD"
                     /locus_tag="ACHKJF_001306"
     CDS             complement(20893..21306)
                     /gene="yedD"
                     /locus_tag="ACHKJF_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP0948691.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21493..22698
                     /gene="yedE"
                     /locus_tag="ACHKJF_001307"
     CDS             21493..22698
                     /gene="yedE"
                     /locus_tag="ACHKJF_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP0948692.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            22695..22928
                     /gene="yedF"
                     /locus_tag="ACHKJF_001308"
     CDS             22695..22928
                     /gene="yedF"
                     /locus_tag="ACHKJF_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP0948693.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            23037..23705
                     /gene="yedK"
                     /locus_tag="ACHKJF_001309"
     CDS             23037..23705
                     /gene="yedK"
                     /locus_tag="ACHKJF_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP0948694.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(24098..24412)
                     /gene="fliE"
                     /locus_tag="ACHKJF_001310"
     CDS             complement(24098..24412)
                     /gene="fliE"
                     /locus_tag="ACHKJF_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP0948695.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            24627..26285
                     /gene="fliF"
                     /locus_tag="ACHKJF_001311"
     CDS             24627..26285
                     /gene="fliF"
                     /locus_tag="ACHKJF_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP0948696.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            26278..27273
                     /gene="fliG"
                     /locus_tag="ACHKJF_001312"
     CDS             26278..27273
                     /gene="fliG"
                     /locus_tag="ACHKJF_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP0948697.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            27266..27952
                     /gene="fliH"
                     /locus_tag="ACHKJF_001313"
     CDS             27266..27952
                     /gene="fliH"
                     /locus_tag="ACHKJF_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP0948698.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            27952..29325
                     /gene="fliI"
                     /locus_tag="ACHKJF_001314"
     CDS             27952..29325
                     /gene="fliI"
                     /locus_tag="ACHKJF_001314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP0948699.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            29344..29787
                     /gene="fliJ"
                     /locus_tag="ACHKJF_001315"
     CDS             29344..29787
                     /gene="fliJ"
                     /locus_tag="ACHKJF_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP0948700.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            29784..30911
                     /gene="fliK"
                     /locus_tag="ACHKJF_001316"
     CDS             29784..30911
                     /gene="fliK"
                     /locus_tag="ACHKJF_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0948701.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            31016..31480
                     /gene="fliL"
                     /locus_tag="ACHKJF_001317"
     CDS             31016..31480
                     /gene="fliL"
                     /locus_tag="ACHKJF_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP0948702.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            31485..32489
                     /gene="fliM"
                     /locus_tag="ACHKJF_001318"
     CDS             31485..32489
                     /gene="fliM"
                     /locus_tag="ACHKJF_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP0948703.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            32486..32899
                     /gene="fliN"
                     /locus_tag="ACHKJF_001319"
     CDS             32486..32899
                     /gene="fliN"
                     /locus_tag="ACHKJF_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP0948704.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            32902..33267
                     /gene="fliO"
                     /locus_tag="ACHKJF_001320"
     CDS             32902..33267
                     /gene="fliO"
                     /locus_tag="ACHKJF_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP0948705.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            33267..34004
                     /gene="fliP"
                     /locus_tag="ACHKJF_001321"
     CDS             33267..34004
                     /gene="fliP"
                     /locus_tag="ACHKJF_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP0948706.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            34014..34283
                     /gene="fliQ"
                     /locus_tag="ACHKJF_001322"
     CDS             34014..34283
                     /gene="fliQ"
                     /locus_tag="ACHKJF_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP0948707.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            34292..35077
                     /gene="fliR"
                     /locus_tag="ACHKJF_001323"
     CDS             34292..35077
                     /gene="fliR"
                     /locus_tag="ACHKJF_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0948708.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            35367..35990
                     /gene="rcsA"
                     /locus_tag="ACHKJF_001324"
     CDS             35367..35990
                     /gene="rcsA"
                     /locus_tag="ACHKJF_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP0948709.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(36034..36222)
                     /gene="dsrB"
                     /locus_tag="ACHKJF_001325"
     CDS             complement(36034..36222)
                     /gene="dsrB"
                     /locus_tag="ACHKJF_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP0948710.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            36385..36612
                     /gene="yodD"
                     /locus_tag="ACHKJF_001326"
     CDS             36385..36612
                     /gene="yodD"
                     /locus_tag="ACHKJF_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP0948711.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            36910..37725
                     /gene="yedP"
                     /locus_tag="ACHKJF_001327"
     CDS             36910..37725
                     /gene="yedP"
                     /locus_tag="ACHKJF_001327"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP0948712.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIVENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(37722..39416)
                     /gene="dgcQ"
                     /locus_tag="ACHKJF_001328"
     CDS             complement(37722..39416)
                     /gene="dgcQ"
                     /locus_tag="ACHKJF_001328"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP0948713.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(39587..39769)
                     /gene="yodC"
                     /locus_tag="ACHKJF_001329"
     CDS             complement(39587..39769)
                     /gene="yodC"
                     /locus_tag="ACHKJF_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP0948714.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(39848..40765)
                     /gene="yedI"
                     /locus_tag="ACHKJF_001330"
     CDS             complement(39848..40765)
                     /gene="yedI"
                     /locus_tag="ACHKJF_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP0948715.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            40938..41858
                     /gene="yedA"
                     /locus_tag="ACHKJF_001331"
     CDS             40938..41858
                     /gene="yedA"
                     /locus_tag="ACHKJF_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP0948716.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(41847..42317)
                     /gene="vsr"
                     /locus_tag="ACHKJF_001332"
     CDS             complement(41847..42317)
                     /gene="vsr"
                     /locus_tag="ACHKJF_001332"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP0948717.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(42298..43716)
                     /gene="dcm"
                     /locus_tag="ACHKJF_001333"
     CDS             complement(42298..43716)
                     /gene="dcm"
                     /locus_tag="ACHKJF_001333"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP0948718.1"
                     /translation="MQENISVTDSYSTGNATQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(43783..44478)
                     /gene="yedJ"
                     /locus_tag="ACHKJF_001334"
     CDS             complement(43783..44478)
                     /gene="yedJ"
                     /locus_tag="ACHKJF_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP0948719.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(44518..44799)
                     /gene="drpB"
                     /locus_tag="ACHKJF_001335"
     CDS             complement(44518..44799)
                     /gene="drpB"
                     /locus_tag="ACHKJF_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP0948720.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT        11231 a        11633 c        11156 g        11316 t
ORIGIN      
        1 aaaaaacgtt ttataaaatc atcacttcat catgaattca aattcattga ttaatatcaa
       61 caagatacaa aaagcactat cattaaaatt cattgcagtt acattgattt catcaatgaa
      121 atgtaaaaat atataaactt gatgatttaa gcattttctt atacccgttc agacgttatt
      181 cttatttcag atcatcgtca gaattgactc cacgatcaca tttcggaccg gcagaaagga
      241 attattctgc aaacagtaat tatggtgttt tgatttatct tgcacctctc cacttctgga
      301 tataaggata ttaggtatgg caaccgctgg aatgcttctc aaactcaact ctcaaatgaa
      361 ccgcgagttt tacgcatcca atctctacct tcacctgagt aactggtgtt ctgaacagag
      421 tctgaacggc accgccactt tccttcgcgc ccaggcacag agtaatgtga cccaaatgat
      481 gcgcatgttt aactttatga agagtgtcgg cgctaccccc atcgttaaag ccattgatgt
      541 tcccggtgaa aaactgaact ctctggaaga actgttccaa aaaacgatgg aagaatacga
      601 gcaacgttct agtacgttgg cacagttagc cgatgaagcg aaagaactga atgatgattc
      661 aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag catgatggtc tgttgctgca
      721 aaccattctt gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg tgcagaccga
      781 ccaacatgtt ctgaatgtcg tgtcacacca gctgcattga tcatcatcgg cgctaatgca
      841 ttgcgccgat gaaggttttg agaaaccgct gcctcatctg tttgaagcag cggttttttt
      901 aatgggattc accctgtggg gtaaacttga gttcaataag cgcgatggct ttttggattg
      961 cccgcatggt gaccgggtct gcggcggcgg gatggttagt aaagtcgata ttcttcagct
     1021 gactggacat ttttcacgaa cttcaacggg cgcgattaca tcgagaacat ccagaatttg
     1081 tttgataacc aactggcaag caaccacatc agaaaccagt tcctgatcgg cattcagcgg
     1141 ctgggacatc gtaaactcct gatagcattt tgaaagccgt tatagtagcg gcttcacatc
     1201 ttcagcgata gtcacatcca ccgtcatcag gacacaaaaa aacctgccgg agcaggtttt
     1261 ttattatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat agttattgaa
     1321 catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg ttatacaggt
     1381 gttattctct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt tatgtgatac
     1441 aaatcacata aatacccctt taatgttata aaaatgataa tcaaaaaaca gcccccctat
     1501 ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc ggccattgtc
     1561 gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga ccatgagact
     1621 tctgattctg actctttcat taataacgct ggcggggtgt acggttactc gccaggccca
     1681 tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc aggctttttt
     1741 gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg catgccagca
     1801 ggaaggctat acccacgcgg tcccctttgg tcagccagta ggcaactgta gcctttttgc
     1861 gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc attccgcctt
     1921 ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta aagaaatagc
     1981 ggtaatgcgt tttattccca ttcgtatttt taataattga agtttatatt ttaccttttg
     2041 caaataataa aataacaaat tatagtgacg ccacagcaac aaatataacc tttgtggagc
     2101 actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa cctggaactg
     2161 tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac cttcgaaggt
     2221 gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca gcgtctgttt
     2281 gattacctga ctgataccgg caatttaccg cgtattaata ccgttgaatc tccgtttgct
     2341 gaatattcct cacttgatga attattccag gaaacctata aacacgaaca attaatcacg
     2401 cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc aacatttaat
     2461 ttcctgcaat ggtatgtttc tgagcagcat gaagaagaga aactgttcaa atcgattatt
     2521 gataaattaa gcctggcagg caaaagcggc gaaggtctgt attttatcga caaagaactc
     2581 tctaccctcg acacacaaaa ctaatgctct caggcggcag gttttctgcc gccgtttcaa
     2641 atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc ttcttctgaa ggtataaatt
     2701 tgccttttgc tgccagaaac gctaccagct ctcccgcagt catcccttct gccgagcagg
     2761 tgtgaaaacg tgcctgttca ccaaaacgcg ctttaattgc tgcttccaga ctggcatgcg
     2821 tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga atagagtcca
     2881 tcgtccctcc tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac tttgcgccag
     2941 cgcaggactg aatgcttttt attgtacatt tatatttaca ccatatgtaa cgtcggtttg
     3001 acgaagcagc cgttatgcct taacctgcgc cgcagatatc actcataaag atcgtcagga
     3061 cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg ggaaccacaa
     3121 ttggcgcagg catgctggca atgccgctgg ctgcggccgg tgttggtttt agcgttacgt
     3181 taatcttgtt gattgggctt tgggcgttga tgtgctacac ggcgctatta ctgctggagg
     3241 tgtaccagca tgttccggca gataccggtc ttggcacgct ggcaaaacgc tatctgggac
     3301 gctacggtca atggctgacg ggcttcagta tgatgttctt aatgtatgct ctgactgcgg
     3361 catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg acaggtattt
     3421 caatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc gtggtttgtg
     3481 ttggaacatc gctggtcgat ttatttaacc gttttctgtt cagcgccaaa attatttttc
     3541 tggtggtgat gctggtattg ctgctaccgc atattcacaa agtgaatctt ttaaccctgc
     3601 cgttgcaaca ggggctggct ctgtcagcaa tcccggtgat ttttacctca tttggttttc
     3661 acggtagcgt gccgagtatt gtcagctata tggatggcaa cgttcgtaag ctacgctggg
     3721 tgtttataac cggtagtgcg atccccctgg tggcatatat tttctggcag gtggcaacgc
     3781 ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgct ggattaaacg
     3841 ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg gcagtgcatt
     3901 tatttgctga tttagccctc gccacgtcat ttctcggcgt tgcgttaggc ttatttgatt
     3961 atctggctga tttatttcag cgttcaaata ccgttggtgg acggttgcaa actggtgcaa
     4021 ttacctttct gccgccgttg gcgtttgcac tgttttatcc acgaggattt gtgatggcgc
     4081 tgggttacgc cggtgtggcg ctggcggtac tggcattgat tatcccttcg ctgttgacct
     4141 ggcaaagcag aaagcacaat cctcaggcgg gttaccgggt gaaaggtggt cgtccggcgc
     4201 tggtggtggt gtttctctgt ggtattgctg tgattggcgt gcaatttttg attgcggcag
     4261 ggttgttacc agaagtgggg tgatcagata gcctcaaatt ccttattggg tgccagaatt
     4321 aacgctgaca cccaatttga cctcttaatg caggcagcac tgcttaaatt tcttaccact
     4381 accgcacggg caaggatcgt tacgccccgg tttctcttct gctttgatcg gttgctgaac
     4441 agctttttcc tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg ccagtcgaat
     4501 ggcatctacg ctctcttcaa acgcttctgg cgacattttc tctacccgct cgaagttttc
     4561 ctcagtaccg tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg gcaacgttga
     4621 ccagtcagaa agtgccacgc cccgcatata gccaaagcac cactcctcaa caatcgtcag
     4681 ctcgctgcca tcaacttctc gcaagccgaa taacggctca aactgctccg ggaattcgtt
     4741 cagacgctct gcggtatcgg ccatatgttg aaaagccaga ttcataaagc gcgtcatctc
     4801 tttctctgac gcccagcgcg gcacatagtc agccccaccc cacacggcaa ccagccactg
     4861 ttccggttca atctcttgcg gagaactcaa caccgccgtc aataaaccgt ccagctccgc
     4921 cacatcaagg atggcgtggt cagtgttgta tttggtcaga atatcgtcca gccattccaa
     4981 ctcactttcg tttaacggtc ccgttttcat acgcttttcc ttgtggatct caactcgcca
     5041 gcacctatct tacatgccgg tccgtatcag agatactttt tgagtggctt tgctggtgat
     5101 taaaaattag ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc cgaaggtggt
     5161 ttcacgacac tgcttattgc tttgatttta ttcttatctt tcccatggta cccggagcgg
     5221 gacttgaacc cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt ctaccgattc
     5281 caccatccgg gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg gactagacgg
     5341 atttgcaatc cgctacataa ccgctttgtt aacgcgccaa attcttcagg cctttcagcc
     5401 agacatccgc ttgacgccga tgtcttttaa actggagcgg gaaacgagac tcgaactcgc
     5461 gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgcat tcatcaagca
     5521 atcagttaat cacttgattt tattatcgtc tggcaatcag tgccgccgtt cgatgcgttg
     5581 cattctactt acctggcgcg atgagtcaac gatatttttc accacttttg atcgtttgct
     5641 gaaaattacg ccgaaacgat cactgatcaa gcaaatctgc acgcgcagcg ctcaaatatt
     5701 gcaacattga ccacagagtc agtaccgcag ccacaaagaa aagtgcaata ccggcgtact
     5761 caacccaaat gttcggacgc cacagcagcc aggccaacgc caccatctgg gcagtggttt
     5821 tcactttccc aatccaggag acagccacgc tactgcgttt acccaactcc gccatccatt
     5881 cgcgtagcgc agaaataata atttcacggg cgatcatcgt tgccgccggt agggtcaccc
     5941 accagctgtg ataatgctcg gttaccagca ccatggcgat agccacgaga actttatctg
     6001 ctacagggtc aaggaaagca ccaaaccggg tactctggtt ccagcggcgt gccagaaaac
     6061 catcgaacca gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca aacggcgacc
     6121 aggtgacagg cagataaaag accaatacaa agaatgggat aaggatgaca cggaacagtg
     6181 taagcaacgt agggatatta aattgcataa tgacgggtaa ctatctgttg tcagtaagat
     6241 tacccctatg ttgctacaga gacatcaatg tttcaacgac cagaagatct tttctgccag
     6301 accttgcgaa atacccggca cttttgcaat ttcctcgacg ctggcgttac gtaaaccttg
     6361 caaaccgccc atatatttca acaacatttg ccgacgtttt ggcccgacgc cttcaatggt
     6421 ttccagggag ctggtatttt tgaccttcgc ccgtttttta cggtgcccgc caatcgcgtg
     6481 atcgtgtgat tcatcgcgaa tatgctggat aacatgcagc gcgggagaat ctggcggcaa
     6541 actgaatccc tcaccttccg gctcaaagaa cagtgtttcc agcccagcct tacgatctac
     6601 tcctttggca acgccaagta gcagtggatg atttttatcc catgagacat ccagttcggc
     6661 gaagacattt ttcgcctgcg caagctggcc tttgccgccg tcgataagga tcacatccgg
     6721 gatcttactg tcgtcaatgg ctttaccata acgccgacgc agcacctgat tcatcgccgc
     6781 ataatcatcg cccggcgtga tgcctgtaat gttatagcgc cgatactccg cacgcagcgg
     6841 gccgttagca tcaaacacca cacaggaagc gacggtttgt tcgcccatgg tatggctgat
     6901 gtcaaagcac tccatccgct tcacttccgg caatttcaac acgctggcaa gcgcggtcag
     6961 tcgctggtga acggtagatt gctgcgaaag tttgctggtt aaggccgtcg ccgcattggt
     7021 gcgcgcgagt ttcagataac gcgccctatc gccgcgaggt ttggtttgaa cattaatctt
     7081 gcgtcccgcc agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa gattaaaatc
     7141 gagcaggatc tcacccggta aggtgcgcat ctggctgcct tgtaaataga actggcctac
     7201 gaaggtttct accacctcgc tcagttccgt accgccaggc actttcggga aatagctgcg
     7261 gctgccgagc actttgccct gacgaatgaa caatacgtgg acacaagcca tgcccgcatc
     7321 gaacgccaca ccaataacgt cgaggtcgtc gccggtattg gaaacgaatt gtttttcggt
     7381 gacgcgtcgc accgcctgaa tttggtcgcg aatacgtgca gcttcttcaa actccagatt
     7441 ctggctggca gtttccatac gactaatgag ttgcgtaagc acctgatcat ctttgccaga
     7501 caaaaacagg cgcacatact cgacctgctg agcgtattct tcttcactca ccagtccttc
     7561 aacgcacggt cccagacagc gccctatctg gtattgcaga cacggacgcg agcgattgcg
     7621 ataaacacta ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg ccagtgtttc
     7681 acgtacggca tagccattcg ggaacgggcc gaaatattca cctttggcat gcttcgcacc
     7741 acgatgcatc gccagacgcg ggtgggtatc accactcagg aagataaaag gatatgattt
     7801 atcatcgcgt agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt tgtgttccag
     7861 caacagcgct tcggtttctg tgtgagtaac cgttacatca atttgctgga tctgggcgac
     7921 cagcgcttcg gttttgcgcg aagcgaggtt gctacggaaa tagctggaaa gccgtttttt
     7981 caggtctttc gctttgccga catagataac cgtaccacca gcatcgtaca tgcgataaac
     8041 gcctggctgg ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat cactcactga
     8101 cttgataatg tctccgcatt acacagacca tggcgaattg ccaggtgagt cagctcaaca
     8161 tcgccatgaa tgtttagttt actgaacata cgatagcggt agctgttcac cgttttcgga
     8221 ctgagattga gctgttctga gatctcattg accttctggc ccttggtgat catcagcata
     8281 atctgcaatt cacgttcaga caaactggca aatgggcttt ctgttttttc tggttcgatc
     8341 tggcttaacg ccatttgttg agcgatgtca gaagcaatgt aacgctgccc tgaatagaca
     8401 gaacgaatcg cactcacgac ttcctgcgga gccgcgcctt tgctgaggta gcccgcagca
     8461 ccggcctgca tgactttcgc tggtaaaggg ttttctgtat ggacggtaag catgatgatt
     8521 ttgacatcag ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc aatgcccggc
     8581 atactcatgt ccattagcac cacgtcaacg gcatttgtcc ggcaccactt aacggcgtct
     8641 tcaccgcacg atgcctcacc gacgactttt atacccttta tatcttccag aatgcgtcgt
     8701 atccctgcgc gcaccagttc gtggtcatca acaagtagaa cgttgatcaa aggaatatct
     8761 ccagaaatag ggataacgct actgatagtt agtcattcgt atattagcgg tttttattgc
     8821 aactttgaaa cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg tcaattcgat
     8881 tgtccacagt tatggtcacg cccgtcacca acggcttacc ggatcgtttc acagtaagcc
     8941 ctacgatgaa atgtgacaaa aattgacttt attcagcaaa aatgaaaatc agcccgctga
     9001 atatgtaaca ttaattaacc cgaacaatac gggcaaaaaa cattcatgat aaaaatattt
     9061 attggtcatt atattaacgt attttatagc actgccgata tcacgctcaa aaaacaacca
     9121 ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattttt caccgcaaat
     9181 tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta ctgccgagaa
     9241 taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc tgatgctgca
     9301 ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac agcttgcgct
     9361 gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa ttaaacaagc
     9421 ctaccgtcag taatataaaa ccggctgaga gcgtgccttt cagccggttt ttgcatctgg
     9481 cacgcagaaa gaaaagcgat caaattaagc cagtagcggc cgcgtaacag gccacctggg
     9541 tcttatttgg tgcattgatt tttttctgca tgtttttctg atggaaattg accgtattct
     9601 cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc gtccatttca
     9661 gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt tcatcattta
     9721 aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat tgcagttcat
     9781 cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag cccagcgccc
     9841 gatttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg tgcgcggctt
     9901 cccataatgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg ccctgactaa
     9961 agttttcagg gttcagcaca ggatcaattg cgagaaaatt ttttgcctga taataactaa
    10021 cccacgactc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat ggtaccgggt
    10081 ggcgaacaca taacgaatag taatcgtact ccagctgctg agcctgaagc tctatttcat
    10141 ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg cgacgccagc
    10201 tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgagagtgag tatcatttat
    10261 aatgataatt caaactatca cataaataag atttatatat aatttatatt attcaggcaa
    10321 tgaattactt ttgcaagcca tcgcattctc ttatgttatt aatgagttat gctgatttgt
    10381 taagcagttt tatcacgctt gaaatggcgt cccgccccga caggtgaatc gtcggggctg
    10441 attttttctt attattgcag cagaaacttc tcgaggaact ggcgggtgcg aggctgctgg
    10501 ggatcagcaa ataacgcttt tgctgccccc tgctcgacta tccgcccctg gtccataaaa
    10561 atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt gcgtcacaat caccatcgtg
    10621 cgcttttcct gcgccagctg gcggattgtg ttcaggactt cgcccaccag ctctggatcc
    10681 agcgctgacg ttggctcgtc aaacaaaatc acctcaggac gcattgccag cgcacgcgca
    10741 atcgcaacac gctgctgttg accgccagac aaacgacgtg gatagctggt ttctttaccg
    10801 gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg tggcctcttc tttcggttcg
    10861 cctttgacga tcaccggccc ttcaataatg ttttccagca ccgtacgatg cggaaacaaa
    10921 ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact ggcgaatcag cgatttttgc
    10981 tgacttaatg aacgtgcagt atcaatagtg atatcgccaa cggtgatcgt tcccgcttcc
    11041 ggttgttcca gcagatttat gctgcgtagc aacgtggttt tgccggaacc actcggaccg
    11101 ataattgcca ccacttcgcc aggctttacc tcgaggtcga taccgtgcag caccgtctga
    11161 ccgtgaaatt ttttcaccag gttcttaact tcaatggcac tcattttggt tctctctcct
    11221 ggcgattaag ttgattctca aaatggttct gcaacgtcga taatactgtc gccatgatcc
    11281 agtagatgag cgaagccgcc aggtacatgg tgaacacctc cagcgtgcgc gaggtaatca
    11341 actgcgcctg acggaacagc tccggcacct ggattgtcgc ggccagcgac gtatccttta
    11401 ccaggctgat aaagctgttc gacagcggcg gcaacgccac ccgcgccgcc tgtggcaaaa
    11461 tcgcgcgacg catagtctgc catggcgtca taccaatact ggcagctgct tcccactgac
    11521 ctttatcaat agaagaaatg gcggcgcgca gtgtttcggc ggcataggcg gcagtgttca
    11581 gcgacagacc aatcattgct gacggaatgg gatctaattc gataccaaac tgcggcaggc
    11641 cgtaatagat cataaacagt tgtgcgatga gcggtgtacc acgaaaaata gagatataaa
    11701 aacgcgccag ccagcgcacg ggccagattg gcgacaggcg catcagcgcg agaataaacc
    11761 cgagcagtaa gccaaaaaac atgccgccga tactgagttg cagcgtatac ccagcccctt
    11821 tcaacaggaa cggcaaggaa tcaataacca gttgtatact ttcttgcatg agcgttcccg
    11881 gagctaaacg tggggatgat aggcgaacag cgcaggcgca ccgccggtat gaataaacag
    11941 aatcggccct tcatctttga agcgtttctg actgataccg ccaatcagcc ccgccatcgc
    12001 ttttccggta tacacaggat caagcagaat gccttccagc cgcgccagca atttcactgc
    12061 ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca aaatagtcat cccagagtaa
    12121 aatttccgct gatgcggtca gctccagttc tttcgcaatc gcctgttgta ggttaaccac
    12181 tttcggcaac tgatcggcaa cggaacgcga cacggtcacg ccaatcagtt cgctttcagg
    12241 catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt ccggcactgc ccgatgcgac
    12301 taccaccgac gaaatattaa ccgccccttc acactgttgc gcgatttcca gcgcactctc
    12361 cacataacct agcgcgccaa gagcattaga accgccaacc ggaatgacat acgggcgaaa
    12421 gccttgtgct tcgactcgcg tcgccagctc ttccagttgg gcattgggat cggtcagtgc
    12481 atcgcacatt tcaatctggg tattgaacag atccagcaac aaacgattgc cgttggttaa
    12541 atagttttct gcggttgtgc caataggatt ttccagcagc gccacgcagt gcagaccgag
    12601 tttcgccgcg actgcggcag tctggcgcac atggttagac tggatcgcgc cggcagtaat
    12661 cagcgtatcg gcaccttcgc gcagggcatc tgccgcgaga aattccagct tacgtaattt
    12721 attgccgccc attgccatgg gtgtgacgtc atcccgtttg atgaaaattt cccgtccaag
    12781 ataatcagaa aagcgcggca gatattcgag cggcgttggc gcgccgataa actccaaccg
    12841 tggaaaacgg gttaaattat gcagtggcat aacagcctcc gatgtgtgtt gttgtgattt
    12901 tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg ctagtgaaag cgcccttttt
    12961 tgtcattatg ctgattattt ggtcacatca gcaccaaacc atttttcgga aagagcttgc
    13021 agagtgccgt ctttttgcat ttccgcaatt gcatcattca ccgctttcag caggtcttca
    13081 tttcctttac gcagcgccac gccagactcc tgacgggaga atgcttcacc ggttactgcc
    13141 agcgtatcgt tggttttctt caccagatcc agcgccgcca gacgatcaac gaggatcgca
    13201 tcgatacggc ctacgcgcag atcctgatat ttggtcgggt catcatcata ggtacgcaca
    13261 tcgacgccct gaacattctg ccgcagccac tcttcatagt tggtgcccag accgacaccc
    13321 acttttttgc ctttcagatc agcagccgtt ttaatggtgc cttcgttacc ttttttcacc
    13381 agcgcctgaa taccggaaat ggtgtaaggg gtagagaaat cgtatttttt cttgcgctcg
    13441 tcagaaatgg tgacctgatt aatcaccaca tcaatgcgtt tagagtccag cgacgccagc
    13501 ataccgtccc atttggtcgg ttttagtgac gcctcaacgc caagatgttt tgccagctgt
    13561 tgggcaaatt ccacttcaaa accggttaat ttgccgtcat ccccctgaaa actgaacggc
    13621 ggataagttc cttccagccc taccagcagc gtgccgcgct ctttgacttt attaagcaga
    13681 ccttcatctg caaaactttt aacgctcatg cccgcaacca gcgccacggc catcacaccc
    13741 atcaatgcct gacgtcccag atgtgctaat ttcatattca ccccgaatgt tgttatgtct
    13801 gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa ctccgctaca tcttattctt
    13861 atttaatata tatcagaagc aggcaggctg gaggatttct gcacaagtcc ggtacacgtt
    13921 tgacgctgat agtgttgata cttccgtaat gcgatgcggt aattgttggt gctggtggtg
    13981 ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct gcagctgatc gagggaaata
    14041 ttttgctcgt gtagatgatt ccccaaacgg cgcagacgaa cgacatattc acgcaccgta
    14101 cctgggctca tttcagtttg ctcaaaaaga aattgcttaa agccgataat atcgaaaaaa
    14161 tcactctgcg ttttgcaatg taaatcaccg caaaatcggc acaatgccgc ccatgattgt
    14221 tgttccactt gccagccatt ttcatcaagc agcgtgtcca ggcgggaaat ctcgatttta
    14281 ttcactattt tgccgtcgcg aactaaggta atgcgatcga gtaatttacg gcaatgcgcg
    14341 caatgggtct ggctgtgttt aaagtcttta agatagcggc ttaatggccg tcttttcagg
    14401 tgctgcacca tcattaagaa ctcctggtag tcaaaattaa agtgcggcat ttactgacgt
    14461 tataacttac ccagtttagt gcgtaaccgt ttaatggcct ggctgtgtaa ctgactgacc
    14521 cgcgattccc cgacctccag caccgcgcca atctctttga gattcagctc ttcctggtaa
    14581 tagagggtta ataccagttt ttcgcgctcc ggcaacgttt cgatagcttc catcactcgc
    14641 tggcgcagat tactgtccag cagttgttgt agcgggtttt ctcgctgatg atcatcagta
    14701 accagttcga tgctatcgcc gtgctcttcg cgccactcat cgtaggagaa gagctggctg
    14761 ttattggtgt cgagcaacat ttggcgataa tcggcaatat cgatccctaa acgttccgct
    14821 acctcagttt ccgtggcgtt gcggccaagt tcctgctcca gttgccctat tgcctgtgcc
    14881 acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt cacggctgcg aagttcatcc
    14941 agcatagcgc cacggatacg ctgcactgcg taagttgtaa atgccgttcc ttgtagggcg
    15001 tcatagcgtt cgacggcatt aagtaaccca atgccgcccg cctgtagcag atcgtcaagt
    15061 tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt cgtgacgcac cagcgggaca
    15121 taacgctgcc acagcgagtg tttatccatt acaccttcag cggtatagag tgaattcacg
    15181 ataaacagcc ctgcgttata tgagttatcg gcatgattat ccgtttctgc agggttttta
    15241 atcggacgat tagtgggtga aatgaggggt tatttggggg ttaccggtaa attccatgca
    15301 gaaaaaaccc cgccggtggc ggggtttgag cgataagtgt aaattaacgc agcagagaca
    15361 gtacgttctg cgtggtctgg ttagcctgtg ccagaacaga agtacctgct tgttgcagga
    15421 tctgcgcacg agacatgtta gacacttcgg tcgcgtagtc agcatcttcg atacggctac
    15481 gggcagaaga caggttgttt acggtgttgc caaggttggt gatggcagag tcgaaacgat
    15541 tttgaattgc acctaggtta gaacgcaacg agtcaacctg agacagtgct ttatcgatag
    15601 cagccaaagg atcttctgtt ttctgcgtag aaattgctgc aatagaatca gttttcaggc
    15661 tcttaccttg taaatcacta acactgtaag ttttgccatc ggttgcagtt acaacttcgg
    15721 ttttgccatc gcttccaccc agttcaactt taacattgtc gtgtttgaca ccgtcagcat
    15781 cagtgtaaga cattgtttta acggaaactg ctcctgtttt ctgatcaaca ttcgctgcat
    15841 actgtttacc gtcatcacca atcaacgcaa atgaaccagt atctttgcca ttggcgtctt
    15901 ctaacttaaa gagttttgca cctgtgatag aagcggttgc ggtatcagta cccaactgaa
    15961 catcaatagc ccccttccct ttcaccgttg tgacttcctt taaattacca gcttgaggct
    16021 gggtacccgc tagagccccc agcttcagct tacctgcatc aggagatgtc ggatcatcct
    16081 ccacagtaac ttcataataa ccattttcag cgggtttatc tgaaccggca actttaacgt
    16141 acaacttacc gttagcatca gacataatat taccgtcacg ttggcccgga gtcgtagtgc
    16201 ctttaactgt agctgtaata tctgcatcaa gtgtattagc gtcagcaagc ttaactgtcg
    16261 atccaaaata ggttgtttca gccgttgcgg cttttaattt agtaggatct ttaataccaa
    16321 aaccagataa tcccagtgtg tcggaattaa tttcctgcag tttaatagta attgtttcgc
    16381 cgtcgtttgc accaacctga atagtcatat cctgatcaga agccagcact ttaacgccgt
    16441 taaactgagt ctgaccagaa acacggtcaa tttcactcag acgctgctgg atttcggact
    16501 ggatggaggt caggtcactg tcagagttag taccgttagt tgcctgaaca gaaagttcac
    16561 gaatacgctg caggttgttg ttaatttcat tcagcgcacc ttcagtggtc tgcgcaacag
    16621 aaataccatc attcgcgtta cgggaagcct gagtcagacc tttaatattt gctgtaaaac
    16681 ggttagcaat cgcctgacct gctgcgtcat ctttagcact gttaatacgc aggccagaag
    16741 agagacgttc aatggcggag ctcagagaag actgagattt gttcaggtta ttctgggtca
    16801 acagcgacag gctgtttgta ttaatgactt gtgccatgat cttttcctta tcaattacaa
    16861 cttgatgtta ttgggctgtt gcccacggtt tctcaccgta ccccttgtat cggcacctga
    16921 atttcgaact ttagaaaatt ttagtttctc tcctgttttt tcttaacggc tgaaatatcc
    16981 gtaatatata actctactct gcgcatcatc ttcgtgaatc aactattaaa ctttgcgcac
    17041 aaattgccga taacccgctt atctactgtt tgcaatcaaa aggaattagg tatggcttca
    17101 tttacatctc ttggcgttgg ttcgaatctg ccgctggata cattgctaaa caacctgacc
    17161 atcgctgaaa aaaaacgctt aaacccaatt actcagcaac agagtgacaa taccgcacgt
    17221 ctgacagcct atggcacatt aaaaagtgca ttagaaaaat tccagactgc aaatactgcg
    17281 ttaaataaag ccgatctgtt caaaagcact accgttacca gtagcaatga agaccttaaa
    17341 gtttccaccg aagcaggtgc tgctcccggt atctatacca tcagcgtaac ccaactggcg
    17401 caggcgcaat ctttacgaac tgattccccg acaatcattg catcaacaaa agatgcactg
    17461 ggtgatgagt catcagatac acgcacaata aaaattaccc aagacggtcg taaagagccg
    17521 ctggaaatca agctcaataa agatcagacg tcattggatg agatcagcaa agccattaac
    17581 gatgccgata gtggaatttc cgctagtatc gttaaagtca aagatggtaa ctatcaattg
    17641 gtattgaccg ccagtgaagg tctggccaat aaaatgacca tatctgttga gggtgacagt
    17701 aaattaaatg atctgctggc ttatgacagc aagaccaaca ccgggaatat gaaggaactg
    17761 gttaacgcgc aaaatgcaca gctaaacgtt aacggtatcg atattgaacg ttcaagtaac
    17821 aaaattaccg atgctcctca gggagtgact ctcgatttga ccaaaaaagt cactgacgtc
    17881 cgggtcacgg tgacaaaaag caacgataaa gcaaccgaag caattaaagg ctgggtggat
    17941 tcatataact ccctcattga cacctttaac accctgacaa aatataaaga agtagaccca
    18001 ggtgctgaag cacaagataa aaataatggt gcacttctcg gagacagcgt tgtacgtaca
    18061 attcaaagcg gcatccgcgc acaatttgca aacggtgcca gcgacggtgc cttcaaaaca
    18121 ttaaacgaga tcgggattaa gcaggacggc acgacgggta agctgaaaat tgatgatgat
    18181 aaactcaaaa aagtcctgaa tgaaaataca gcatccgtac gtgaattgct ggtaggcgat
    18241 ggtaaagaaa caggaattac caccaaaatt gccaccgagg taaaaggtta ccttgccgat
    18301 gatggcatta ttgatagcgc acaggacagt attaacgcca cactgaaaaa actgaccaaa
    18361 cagtatctat ccgtcagtgc cagcattgat gacaccgtgg cgcgttacac tgcacagttt
    18421 acgcaattag ataccatgat gagcaaactg aataacacca gtacctatct cagccaacaa
    18481 tttactgcaa tgagtaattc ctgatcacgg aggtcagcat gtataccgcg agcggctcaa
    18541 aagcctatgc acaaattggc gtcgaaagcg ccgtaatgag cgccagccag cagcagctgg
    18601 tcaccatgct atttgatgga gtgctgagcg cactggttag agcgcgcctg tttatgcagg
    18661 acaacaatca gcaaggcaaa ggcgtctctt tgtcaaaagc gatcaacatc attgagaacg
    18721 gacttcgggt gagtcttgat gaagagagca aagacgaatt aacccaaaac ttgattgctc
    18781 tttatagcta tatggtcagg cgcttgctgc aagccaattt acgcaacgat gtctccgcag
    18841 tcgaagaagt ggaagcatta atgcgtaata ttgccgatgc ctggaaagag tcgttactct
    18901 ccccttcttt gattcaggac ccagtctgat gaaccatgca ccgcatttgt atttcgcctg
    18961 gcaacaactc gtcgaaaaaa gccagctcat gttacgcctg gcaacggaag aacaatggga
    19021 cgaactcatc gccagcgaaa tggcgtatgt gaatgcggtg caggagatag cacacctgac
    19081 tgaagaggtt gccccgtcca ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat
    19141 tctcgacaac gaaagcaagg taaagcagtt attacagatt cgtatggatg aactggcgaa
    19201 actggtcggt cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg
    19261 ctttgtgctg gctccgcaag ataacttctc gtgaatctta acgagctaat ggttcgcgaa
    19321 taatccgatt acggctacgc ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat
    19381 cccacgctgt tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa
    19441 ctggccgagc gcgccgacgg ttttaatgat attggtatca atatggtctg gttgccgccc
    19501 gcctataaag gcgcatcggg cgggtattcg gtcggctacg actcctatga tttatttgat
    19561 ttaggcgagt ttgatcagaa aggcagcatc cctactaaat atggcgataa agcacaactg
    19621 ctggccgcca ttgacgccct gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc
    19681 aaccacaaaa tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat
    19741 gaccgtacgc aaattgacga agaaatcatt gagtgtgaag gctggacgcg ttacaccttc
    19801 cccgcgcgtg ccgggcaata ctcgaagttt atctgggatt tcaaatgttt tagcggtatc
    19861 gaccatatcg aaaaccctga tgaagatggc atatttaaaa ttgttaacga ctacaccggc
    19921 gaaggctgga acgatcaggt tgatgatgaa ttaggtaatt tcgattatct gatgggcgag
    19981 aatatcgatt ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg
    20041 atggaacaaa cgcaatgcga cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg
    20101 ttttataaag agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg
    20161 gcggagtact ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc
    20221 aaaaccatgc tgttcgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggg
    20281 cgtgactacg acatgacgca gattttcacg ggtacattag tggaagccga tcctttccac
    20341 gccgtgacgc tcgttgccaa tcacgacacc caaccgttgc aagccctcga agcgccggtc
    20401 gaaccgtggt ttaaaccgct ggcatatgcc ttaattttgt tgcgggaaaa tggcgttcct
    20461 tcggtattct atccggacct ctacggtgcg cattacgaag atgtcggcgg tgacgggcaa
    20521 acctatccga tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag
    20581 cgtttcgccc acggtgtaca gacgttattt ttcgaccatc cgaactgcat tgcctttagc
    20641 cgcagtggca ctgacgaata tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc
    20701 gaaaaaacca ttaatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg
    20761 aaccggcaag agagcgtagt aaccgacgaa aacggtgaag cgaccttctt ttgcaacggc
    20821 ggcagcgtca gcgtgtgggt tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc
    20881 ggggagattt cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca
    20941 ctctgccgtc gggcgatcca cacgctccat cgtcatacca tcgtactcaa tggtgttacc
    21001 gtcacgctca acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat
    21061 caatgtcaac ttgcccggca cggcaatcac ccgctgccac tgacggcaat ccagggtatc
    21121 gccctcttta gtgacaatca ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg
    21181 ccctttggtc tgccagtagc ccgccagcca gtccggcgcg ggggttttca cgacattgtt
    21241 atagttttcg acttcggcac agcccgccag caacatgagc gcgcctgcaa ttgcgagttt
    21301 tttcatcatt cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg
    21361 ccagcctttc caggttcaga gagaaatttg atctaactat tatttttgtg aattattacc
    21421 ccgaaaataa atttgcatga tgtatgattc gcgattcttg tttcaaacct tctgagttca
    21481 gaggctacat tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca
    21541 cccatccctg cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc
    21601 accttttggg ctgtcacggg tgaatttacc cgttggggcg gtcagctcct gcagctgttt
    21661 ggcgtccatg ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta
    21721 acccgcatcg acgggatgat gatcctcggc atgtttggcg gctgctttgc cgcagcgctg
    21781 tgggccaaca atgtcaaact gcgcatgcca cgcagccgta tccgcattat gcaggccatt
    21841 attggcggta ttatcgctgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg
    21901 ttctttaccg gtattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc
    21961 attggttcat ggtttggcgc acgctttacc ctgctgccca tcttccgtat tcccgtgaaa
    22021 atgcagaaag tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt
    22081 tttcgtctcg ggatgctggt ctttttcggt atgctgggct gggcgctgct cacggcgatg
    22141 aaccaaccaa aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa
    22201 cgtgcgcaaa tctgctttac ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat
    22261 atggcgaaag caatcattat cggtatggcg gtgagtgcca tcgggatctt cagttacgta
    22321 cagttaggcg ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat tggtggttta
    22381 ctgtttggtt ttggcatcgt gctggcaggc ggctgcgaaa ccggttggat gtaccgcgcg
    22441 gtagaaggtc aggtgcacta ctggtgggtc ggtctgggca atgtgatcgg ctcaaccatt
    22501 ctggcgtatt actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac
    22561 ctgctgaaaa cctttggccc gatgggtggc ctgctggtga catatttgct gttgtttact
    22621 gcgctgatgt tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact
    22681 gctaaggaga tcgcatgaaa aatatcattc ctgattaccg tcttgatatg gtgggtgaac
    22741 cctgccctta tccggcagtc gcaacccttg aggcgatgcc gcagttaaaa aaaggtgaga
    22801 tcctggaagt ggtgagcgac tgtccgcagt cgatcaataa tattccactg gatgctcgta
    22861 atcacggcta tacggtgctg gatattcagc aagacgggcc gaccattcgt tatttaattc
    22921 agaagtaatc cctcattccc gctgtggcca acagcgggaa tatcgttgcc tctgtaccac
    22981 atagatttta cccgccccct ctcctattct tttcagtgtg ctctcaggag gttgctatgt
    23041 gtggacgctt tgcccaatcc caaacgcgtg aagattacct tgcgcttctc gcggaagata
    23101 ttgaacgcga tattccctat gatcccgaac ccattggcag atacaacgtc gcgccgggaa
    23161 ccaaagttct tctgctcagt gaacgtgatg aacaccttca tctggatccg gttttctggg
    23221 gatatgctcc cggatggtgg gataaaccgc cgctgattaa cgcccgcgta gaaactgcgg
    23281 ccaccagtcg tatgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg
    23341 gctggtttga gtggaaaaaa gaaggcgaca aaaaacagcc ttattttatc tatcgtgctg
    23401 acggacaacc tgtttttatg gccgcgatag gtagcacacc gtttgagcgc ggtgacgaag
    23461 ccgaaggatt tttaattgtc actgcagcag cagaccaggg tctggtagat attcatgacc
    23521 gccgcccgct ggtactgtcg ccagaagctg cgcgggaatg gatgcggcaa gagattggcg
    23581 gtaaagaagc atcagaaatt gctaccaacg gctgtgtgcc agcaaaccag ttcacctggc
    23641 acccggtatc gcgcgcggta ggtaatgtca aaaaccaggg cgcggagtta attcaaccgg
    23701 tttgataagc caggagacta ttgatcaata atactgcgtc ataagaaatc tctattagac
    23761 aaagtagacg ctccttccac atgcgccaca agaaacctct attccagtga cacaattacg
    23821 cctgattatt tccctagccc ggaatatgaa taaaaacatc aacaaagatt aaattattca
    23881 aacgttactg ttgcgtctgc ggtcgtaaca acatccgacg tggtgatgtg tatcaaatga
    23941 ggcctctgat gatcagaggg tttattgcgt atacggatcg tggatggcag aaaactttca
    24001 ggatcaggta tccattttaa accagaaata gggtgagttt gccccatcca tgccccacgc
    24061 gggcagatgg gatgtaatta acaacaggtt acaaaaccta cacttgcatg gacatcactt
    24121 cctgatacgc cgccaccagc ttattacgca cctgaatccc catttgcata gaaactgagg
    24181 ctttttgcat atcggtcatc acatcgttta acgccacgcc gggttcaccg agggtgaatt
    24241 tttctgcctg cgtgcgggca gctgtttgtg tatcgctaat gcgatcgagc gcggcgtgca
    24301 gctgcccggc aaaactaatg gtcggttgcg gcagtgaatc ctgcgcgcgc gcactcatcg
    24361 ccgtagcctg taactggctg ataacccctt caatcccctg tatcgctgac attttcatct
    24421 cctggataat ttctggtagc aaagcctacc agtaagtcaa taagacaaag gcgctaaata
    24481 gcaacaaaaa aacgggttta ttggcggata gaaaaaaacg aaagcacaaa taatgggagc
    24541 gtcaattttt cgagtttgct gacccgggag tgagtcttgt tccactttgc caataacgcc
    24601 gtccataatc agccacgagg tgcgcgatga atgcgactgc agcccagaca aaatctcttg
    24661 agtggcttaa tcgcctgcgt gcgaatccga aaattccatt gattgttgcc ggttccgcgg
    24721 cagtggcggt catggtcgca ctgatcctgt gggcgaaagc ccccgactac cgcacattat
    24781 tcagcaatct ttccgatcag gatggtggcg caattgtcag ccaactgacg caaatgaata
    24841 ttccttaccg cttcagcgaa gccagcggcg ctattgaagt tccggcagat aaagttcacg
    24901 aactgcgtct gcgcctggca caacaaggtt tgcccaaagg cggcgcggtc ggtttcgaac
    24961 tactcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat tatcagcggg
    25021 cgctggaagg cgagctttct cgtaccatcg caactatcgg cccggtaaaa ggggcgcgcg
    25081 tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc ccttctgcat
    25141 cggtgacggt aaatctgtta cccggccgcg cactcgatga agggcaaatt agcgccattg
    25201 tgcatctggt ttccagcgcc gttgctggtc tgccgccggg aaacgtcacg ctggtggatc
    25261 agggcggaca tctgttaacc cagtccaata ccagcgggcg cgatcttaat gatgctcagt
    25321 tgaaatatgc cagcgatgtc gaaggccgta ttcagcggcg tattgaagca atcctgtcgc
    25381 ctattgttgg taacggtaat attcacgccc aggtcacggc gcagctggac ttcgccagta
    25441 aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg gcgcttcgtt
    25501 cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc gtaccggggg
    25561 cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct ccggcaaatc
    25621 aaaataaccg ccagcagcag gcgagcacca ccagcaatag cgggccgcgt agcacacagc
    25681 ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa atgaacgtgg
    25741 gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta caaaaccttg ccagacggta
    25801 aaccattgcc tctcagcaac gaacagatga agcaaattga agatctgacc cgcgaggcga
    25861 tgggcttttc tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg ttcaatagca
    25921 gtgacgaaag cggcggagaa ctgccgttct ggcaacagca agcgtttatc gatcagttgc
    25981 tggctgccgg tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg cggaaagcgg
    26041 tacgcccgca gttaacacgt cgcgccgagg cgatgaaagc tgtacagcaa caggcgcagg
    26101 cccgtgagga agtggaagat gcggtggaag tccgcctgag caaagacgaa caactccaac
    26161 aacgacgcgc taaccaacgt ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt
    26221 ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat aacgatcatg
    26281 agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg cgaagaccgg
    26341 gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaaccctgag cgctgcaatg
    26401 gcgaacgtca cgcagatctc caacaagcag ctaaccgatg tgctggcgga gtttgagcaa
    26461 gaagctgaac agtttgccgc actgaatatc aacgccaacg attatctgcg ttcggtattg
    26521 gtcaaagctc tgggtgagga acgtgccgcc agcctgctgg aagatattct cgaaactcgc
    26581 gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagtgc cgccgatctg
    26641 attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgaa gcgcgcccag
    26701 gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc
    26761 gccacctttg gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt actgaatggc
    26821 ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa
    26881 attatcaacc tgatgaaaac tcagcaggaa gaagccgtta ttaccgccgt gcgcgaattc
    26941 gacggcgagc tggcgcaaaa aattatcgac gagatgttcc tgttcgaaaa tctggtggat
    27001 gtcgacgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc gctgttgatc
    27061 gcgctgaaag gggccgagca gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt
    27121 gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct gtcgcaggtg
    27181 gaaaacgaac agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac tggcgagatg
    27241 gtaattggca gcggcgagga tacctatgtc tgataatctg ccgtggaaaa cctggacgcc
    27301 ggacgatctc gcgccaccac aggcagagtt tgtgcccatg gtcgagccgg aagaaaccat
    27361 cattgaagag gccgaaccca gccttgagca gcaactggcg caactgcaaa tgcaggccca
    27421 tgagcaaggt tatcaggcgg ggattgccga aggtcgccag caaggtcatg agcagggcta
    27481 tcaggaagga ctggcccggg ggctggagca aggtctggca gaggcgaagt ctcaacaagc
    27541 gccaattcat gcccggatgc agcaactggt cagcgaattt caaactaccc ttgatgcgct
    27601 tgatagtgtg attgcttcgc gcctgatgca gatggcgctg gaggcggcac gtcaggtcat
    27661 cggtcagacg ccaacagtgg ataactcggc actgatcaaa cagatccaac agttgttgca
    27721 gcaagaaccg ttattcagcg gtaaaccaca gctgcgcgtg cacccggatg atctgcaacg
    27781 tgtggatgat atgctcggcg caaccttaag tttgcatggc tggcgcttgc ggggcgatcc
    27841 cacactccat cctggcggct gtaaagtctc cgccgatgaa ggcgatctcg acgccagtgt
    27901 cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt aatgaccacg
    27961 cgcctgactc gctggctaac cacgctggat aactttgaag ccaaaatggc gcagttgcct
    28021 gcggtacgtc gctatgggcg attaacccgc gctaccgggc tggtgctgga agccaccgga
    28081 ttacaattgc cgctcggcgc aacctgtgtc attgaacgcc agaacggcag cgaaacgcac
    28141 gaagtagaaa gcgaagtcgt tggctttaac ggtcaacggc tgtttttaat gccgctggag
    28201 gaagtcgaag gtgtcctgcc cggcgcgcgg gtttatgcca aaaacatttc ggctgaagga
    28261 ctgcaaagcg gcaagcagtt gccgctcggt ccggcgttat taggtcgcgt tctggacggc
    28321 agcggtaagc cgctcgatgg cctgccctcc cccgatacga cggaaaccgg tgcgctgatt
    28381 accccgccat ttaacccgtt gcaacgtaca ccgattgaac atgtgctgga caccggcgtg
    28441 cgcccaatca atgccctgct taccgttggg cgtgggcagc gtatgggact gtttgccggg
    28501 tccggcgttg gtaaaagtgt gctgctgggg atgatggcac gttacacccg cgccgatgtc
    28561 attgtcgtgg gtttgattgg tgaacgcgga cgcgaagtaa aagattttat tgagaatatc
    28621 ctcggtgccg aagggcgtgc acgctccgtg gtgattgccg ctccggcgga tgtttctcct
    28681 ctcctgcgaa tgcagggtgc cgcctatgcc acgcgcattg ccgaagattt tcgcgatcgt
    28741 ggtcagcatg tgttgctgat tatggactcc ctcacccgct acgcgatggc ccagcgtgaa
    28801 attgcgctgg cgattggcga accacccgcc actaaaggtt atcccccgtc ggtgtttgcc
    28861 aaattaccgg cactggtcga gcgtgccgga aatggcatta gcggcggcgg atcgattact
    28921 gcattttata ccgtgctcac tgaaggcgat gaccagcagg accccattgc cgactctgcg
    28981 cgggcaatcc tcgacggtca cattgtgctg tctcgccgac tggcggaagc cgggcactat
    29041 ccggctatcg atattgaagc gtcgatcagc cgcgcaatga cggcgttgat cagtgagcaa
    29101 cattacgcgc gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg taaccgcgat
    29161 ctggttagcg tcggcgcgta tgccaaaggc agcgatccga tgctcgacaa agccatcgcc
    29221 ctgtggccgc agctggaggg ctatttgcaa caaggcattt ttgaacgcgc ggactgggaa
    29281 gcgtctctcc aggggctgga gcgtattttc ccgacagtgt cataacccag gagataacgg
    29341 cagatggcag aacatggcgc gctggcgacc ctgaaagatc tggcagaaaa agaggtagag
    29401 gatgccgcgc gcctgctggg tgaaatgcgt cgcgggtgtc agcaggcgga agaacagctc
    29461 aaaatgctga tcgattatca gaatgaatat cgcaataacc tcaacagcga tatgagtgcc
    29521 gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgct ggaaaaagcc
    29581 attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac
    29641 agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc
    29701 acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag
    29761 cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttaattacc gccgacgttg
    29821 acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt
    29881 tgagcgaagc attagcaggc gagacaacta ccgacaaagc ggccccccag ttgctggtgg
    29941 caacagataa gcccacgaca aaaggcgagc cgctggtcag cgagattctt gccgatgcgc
    30001 aacaagcgga tttactgatc cctgtggatg aaacaccgcc tgtcatcaac gacgaacaat
    30061 ccacatcaac accattaacc accgctcaaa cgatgacgtt ggctgcggtg gctggcaaca
    30121 atacggcaaa agacgaaaaa gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg
    30181 ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat gtgccgtcaa
    30241 ccgtgttacc ggcagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacaa
    30301 cagcacagcc tgatgatgcc cccggcacgc cagctcagcc attaacaccg ctggtagcag
    30361 aagcccagag taaagcggaa gtcatcagca caccttcacc ggtgaccgct gccgccagcc
    30421 cgctaatcac tccacaccag acacagccac tgcccaccgt cgccgcgcct gtgttgagtg
    30481 caccgctggg ttctcacgaa tggcaacaat cattaagcca gcatatttcg ctgttcaccc
    30541 gccaggggca acaaagtgca gagttgcgtc tgcacccgca ggatttaggt gaagtgcaaa
    30601 tctccctcaa agtggatgat aaccaggcgc aaatccagat ggtttcaccg catcaacacg
    30661 tacgcgccgc cctggaagca gcgcttccgg tactgcgaac gcagctggcc gaaagtggca
    30721 ttcagttagg gcaaagcaac atcagtggcg aaagctttag tggtcagcag caggccgctt
    30781 cccagcaaca gcaaagccaa cgcacagcaa accatgaacc tctggcgggg gaagaagacg
    30841 atacgcttcc ggttcccgtc tctttacaag ggcgcgtaac aggcaacagc ggcgttgata
    30901 ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct
    30961 caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac
    31021 tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atcccgattc tggtattcat
    31081 taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc
    31141 tgacgacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt
    31201 tacggtcaat ctgggcgatg cggatcgcgt gctgtatatc ggcataaccc tgcgcctgaa
    31261 agatgaagct acacgctcgc ggctgagtga gtatttgccg gaagtccgta gccgtttgct
    31321 gttactgttt tcgcgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct
    31381 gattgccgag attaaaacca cgctttccac cccgcttgtt gccgggcaac cgaaacagga
    31441 tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc
    31501 tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga
    31561 cagccagtgt tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg
    31621 ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc
    31681 gtatggggct gttcaacctg ctgcgtcgta gcccggatat aaccgtcggg gccatccgca
    31741 ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aaccttatcc
    31801 atctgaaacc gctgcgcggc acagggctgg tggtgttttc accgagtctg gtgtttatcg
    31861 ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt
    31921 ttacccatac cgaacagcgc gtcatcaacc gcatgttaaa gctggcgctt gaaggctata
    31981 gcgacgcctg gaaggcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg
    32041 tgaaatttac caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg
    32101 tggagattgg caacctgacc ggcgaattta atatctgtct gccattcagc atgattgagc
    32161 cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact
    32221 ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggagttggtc gccaactttg
    32281 ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga
    32341 tagaaaaacc cgatcgcatc atcgctcatg ttgacggcgt tccggtactg accagtcagt
    32401 acggcaccct caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa
    32461 attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa
    32521 cggcgcaatg gacgatctgt gggctgaagc tttgagcgaa caaaaatcaa ccagcagcaa
    32581 aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca
    32641 ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg
    32701 gatgaccatc aaagagctgt tgcgtctgac gcaagggtcc gtcgtggcgc tggacggtct
    32761 ggcgggcgaa ccactggata ttctgatcaa cggttattta attgcccagg gcgaagtggt
    32821 ggtcgttgcc gataaatatg gcgtgcggat caccgatatc attactccgt ctgagcgaat
    32881 gcgccgcctg agccgttagt gatgaataac cacgctactg tgcaatcttc cgcgccggtt
    32941 tctgctgcgc cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc
    33001 gctgctgcct ggctggtaaa acggttggga tttgtcccta aacgcactgg cgttaacggt
    33061 ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggttgtggt ggtcgatgtg
    33121 gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt
    33181 cccccttcag caccaacgga agagataccg cagcccgatt ttcagtcggt catgaaaaac
    33241 ttgcttaagc gtagcgggag atcctgatgc gtcgtttgtt gtctgtcgcc cctgtccttc
    33301 tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctgc
    33361 ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga
    33421 cgtttattcc ggcaatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg
    33481 gtttattgcg taacgcgctg ggaacaccct ccgcgccacc taaccaggta ttgctggggt
    33541 tggcgctgtt tttgaccttt tttattatgt cgccggtgat cgacaaaatt tatgtcgatg
    33601 cgtaccagcc attcagcgaa gagaaaatat caatgcagga ggcgctggaa aaaggggcgc
    33661 agccgctgcg tgagtttatg ctgcgtcaga cccgtgaggc ggatttaggg ttgtttgcca
    33721 gactggcgaa tactggcccg ttgcagggac ctgaagccgt gccgatgcgc attttgctcc
    33781 cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc
    33841 cttttttgat tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg
    33901 tcccccccgc caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct
    33961 ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac
    34021 ctgaatcggt catgatgatg gggactgaag cgatgaaagt cgcgctggca ctggctgccc
    34081 cgctgttgct ggtggcgctg gtcacgggcc ttatcatcag tattttgcag gccgccacgc
    34141 agatcaacga aatgacgctg tcgtttattc caaaaatcat cgccgtattt attgccatta
    34201 ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta
    34261 acctgccgta tatcatcggg tagcccgtac aatgatgcag gtgacaagcg atcaatggct
    34321 ttcctggcta agtctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc
    34381 gccaattctg agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat
    34441 cacgttcgct attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc
    34501 tctgtggctg gccgtgcagc agatcctgat cggcattgcg cttggtttca ccatgcaatt
    34561 tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt
    34621 tgcgacgttt gtcgatccgg ccagccatct aaatatgccc gttctagccc gtatcatgga
    34681 tatgctggcg ttactgctgt tcctgacatt taacggtcat ttatggttga tttcactgct
    34741 ggtcgatacc tttcacaccc tgccaattgg tggcgaaccg ttgaacagca atgcgtttct
    34801 ggcactcacc aaagcaggga gtttgatttt ccttaacggg ctgatgctgg cgttaccgct
    34861 cattactctg ctgctgacac tgaatctggc attaggttta cttaatcgta tggccccgca
    34921 attatccatt tttgttattg gatttccatt aactctgact gtcggtatct ctttaatggc
    34981 ggcattaatg ccgttaattg cacctttttg cgaacattta ttcagtgaga tgtttaattt
    35041 gctggctgat attattagtg aattgccatt aatataattc cgtaacgttt atcatgttat
    35101 cctaaggatt atccgaaaaa taatacctgc gaacatcttc caggatactc ctgtggcgaa
    35161 atatttgttt taagctcact cacatatcgc aacatttact ttactttaag acaattccag
    35221 gcaaattata caacacttta cgggatagta agtccgcctg aaaaatcgcg agagtggcgc
    35281 attaggtgac ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat
    35341 cgttacgcat tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca
    35401 cccgactagg tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg
    35461 acattgaaac cgttgatgac cttgccatag cttgtgattc acagcgccct tcagtggtgt
    35521 ttattaatga ggactgtttc atccatgatg cttctaacag tcagcacatc aagcacatca
    35581 ttaatcaaca tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg
    35641 atgaatatct attggtcaga aaaaatttat tgataagttc taaatcgatt aaaccggaat
    35701 ctctcgacga tatccttggc gatattctga aaaaagagac aacgataacc tcttttttaa
    35761 atatgccgac gttatcattg agccgaaccg aatcgagtat gttgcgaatg tggatggcag
    35821 gtcagggaac gattcaaatc tctgaccaaa tgaatatcaa agccaagacc gtttcatcgc
    35881 ataaaggtaa tattaaacgt aagatcaaaa cgcataataa acaggttatc taccatgtcg
    35941 tccgactgac ggataatgtg actaatggta tttttgtcaa catgcgctaa cacattctga
    36001 ctggtggttt cccaccagtc aggctgaata agattactct gctttctcca caaagatacc
    36061 gtcctgatgc cctgcttcat taaagaacca gatgccgagc gggtagtctt ccagcgaaac
    36121 caggtacatt gtgccttcac taaactcctc aactgccagt accacgccag gacgacgcgg
    36181 accgccatcc gttttgactg ttacccgatc attcaccttc atcgttttcc tcctgtggct
    36241 ttgtgccagt gtagaacaat ttcgtttttc tggcagcgcc aggcgcgcgc gagtgctgat
    36301 tttctcgacg gtctatactt aagagatgcc aggcggactt aacgactggc ggcaacaaca
    36361 gagtaacggt tgcgaggaaa gatgatgaaa accgcaaaag agtacagcga taccgcaaaa
    36421 cgtgaggtca gcgtcgatgt cgatgccctg ctggcggcga tcaatgaaat tagcgaaagc
    36481 gaagttcatc gcagccagaa cgattctgaa cacgttagcg tcgatggacg tgaatatcat
    36541 acatggcgtg aattggcgga tgccttcgaa ctggatattc atgacttcag cgtctctgaa
    36601 gtgaatcgtt gaatgcacaa taaaaaaatc ccgaccctga gggggtcggg atgaaacttg
    36661 cttaagcaag aagcacttaa aaaattcgtt acaccaggaa atctgatgtg ttcatcacct
    36721 tatccgcaat ttttttcgct gacaagaaaa tattcgcgat atgaatgatt aacgctactt
    36781 atgaatagaa atgtgacgtg cgtcacccct tggtggctct ggcgtcaacg ggatcgctcc
    36841 cgaaaaaatc atctttgatt atttgtgctg ttttgagaat catcctggta aggggagaac
    36901 tgcgcgacca tgttttcaat tcaacaacca ctactggttt ttagcgatct tgatggcacc
    36961 ctgctggaca gtcatagtta tgactggcaa ccggctgccc cctggctcag ccgtttacgc
    37021 gaagcgaatg ttcccgtcat tctctgtagc agtaaaactt cagcggaaat gctgtacttg
    37081 caaaaaacgt tggggctaca agggttacca ctgattgtcg aaaatggcgc agtgatccag
    37141 cttgctgaac aatggcagga tatagacggt tttccacgca tcatctcagg tattagccat
    37201 ggcgaaatca gccaggtttt aaatacgcta cgcgagaaag aacattttaa attcacgact
    37261 ttcgatgatg tcgacgatgc aaccatagcc gaatggacgg gattaagccg tagccaggcg
    37321 gcgctgacgc agcttcatga ggcgtcggta acgctaatct ggcgcgacag tgacgagcgt
    37381 atggcacaat ttaccgctcg tctgaacgaa ctgggcttac agtttatgca aggtgcgcgc
    37441 ttctggcacg tcctggatgc ctctgccgga aaagatcagg ctgccaactg gattatcgcg
    37501 acctatcaac aattgtcagg caaacgccca accacacttg gcctgggcga tgggccaaac
    37561 gatgcgccct tactggaggt aatggattac gcggtgattg tgaaagggct aaaccgtgaa
    37621 ggggtgcatc tgcatgatga ggatccggcc cgcgtctggc gaacgcagcg tgaaggaccg
    37681 gaaggatggc gtgaagggct ggaccatttt ttctccgccc gttaagcgtt atcgctcgcg
    37741 aatacccgat tacgcccagc ctgtttagcg agataaagcc gacggtcggc cagtgactgg
    37801 agttgttcaa aatcataatc accggtttcc tcgctgctac ttacccccaa cgaggcactg
    37861 atgcgtatcg tcgtactctt agcaatcaac atctcttttt catttaactt caggcgaata
    37921 cgttctgcga cttccgcagc ctccgtcaga ctcgcgcctg gcagaatcac acaaaactcc
    37981 tcaccaccga cccgcccggc aacgtcctgc gcacgcaagg aactgctaat taatccggca
    38041 gcatgagaaa gaacacggtc gcccgcctga tgaccaaagc ggtcattaat cgctttaaaa
    38101 tggtcaagat cgacctgaat gacagaaaaa ggatgttggt gcgtctgaca caatttcgcg
    38161 agcggacggg ctttttcaaa cagtgcgcca cgattatata aacgggttaa ggtgtcgtgc
    38221 cacgcctgcc actgcaacga gctttgcaga acatacatgt tgctaaccat ccggcgaatc
    38281 acataccagg agatgagtaa catggtggta aagagcgccc acagcagggt taatgcaatg
    38341 ctgatactgc cgaaatcgcc gcgcacgcct tcgcttagcg tatggacgcg caccagcaca
    38401 ccgtcgaaat ggtccagacg ttcccagcta acatagcgac tgtccatacg aatgccgccc
    38461 cgcgtgtcat gttccatcgc ctgcgccagc aaggccagtt cacgaggatc aaaaatattc
    38521 cctgttggat ggtcaggatt ggaagaggtc aaaaatctca gcttactgtc atagagctga
    38581 tactcaccat cgaggttttt atcgatggcg tttcttaaaa attgctgcat agtacgtacg
    38641 ggaatactca tccccagcac gccataccag tagttattac tgtctaccgg aacactgacg
    38701 gtaacctgcg gttcagtatt gctggcgtgt tccggttgcg aagtgaacca gcgtaccgcg
    38761 cggtgacgat tttctcgttg tgaatggccg ataaaccagg gttgggtgac atagccgtaa
    38821 taacgcgttg gaacattgcg cgtaaacaag gtcggctgcg tcgaaacgta aaatccggca
    38881 cgcgagacat acatcgcctg ttcaaccatc gacgaggagt tgtgcgccag tcgcagcaag
    38941 taaccaactt ccagtgcagc ggtaatttca ttgtcgaggc tttcattttc gcgagacagg
    39001 agatttccct cgctgactaa tgcatccgat acgccattga ctggcagggt gcgtcgtcgg
    39061 ttgagttcga ttttccaggc gtgctcgtcg cgatgctgtt cgaactcggt tacagcatca
    39121 cgcagagagg tgaaatccag tggcgctacg agagcttcgc gcatgccatt acgcaaaaag
    39181 atcagtttgt cgacgttata ctgcaaatgc ttatcgagcg cgttggctac gttttccaga
    39241 tgattacgct ggctggagat ataggcatct tccagcacca ctacttcacg ccaggtgagc
    39301 aaggtggaaa aaagcaatac cacgataaag cagagattaa cgacatgacc aggccccagg
    39361 cggcgtgcga gttttttcaa ccagctctgg ttttccattt ttgtctcgtg ctgcaccctg
    39421 acgactccca acctgctttt ttatgattct ggcataacct ggcgatagcg taccgcaaaa
    39481 cagcgataat tttataatgg tggcatgata agacgcgact atcattcatt gcaggcattg
    39541 gcacaccagg ccggatgtga tccggcctgg tgttaatgat accctttcaa acttcttccg
    39601 cagaacgact cccctccccc ggaacaagct cggtttcatg aaaagcttcc cgcttgaccc
    39661 cgtaaccgtc ataccaacga cactcaacca taccgctgga gtatccggtg acaatcatcc
    39721 gcgggccgcc ctctttaacc gtaacttcct cactaaccat aaagctcata ctcgcctcct
    39781 tttttcgagt gaaacttgtt caccttagtt gaagatggcg aattttgcat aatagcctgt
    39841 gcgagtatta atgtgcctga ccgcgcattt tcgctacggc tttcactccc agcaccacga
    39901 tgccgccgat gataaatcca agaatcagat ttaaaacagt cggtaatatc atcgccacca
    39961 ctgcactttg ctgcccggcg aaatgttcaa tggcgtgatg cagcggcgca ataccatgta
    40021 ccacaatacc gccgccgacg aggaacatcg ccagcgtgcc gacaatcgat aacgccttca
    40081 tcaaccaggg cgcaatgatc aataaccctt tacctaatgc ctgcatcagc gcgctggatt
    40141 tttccgccag ccaatacccc aggtcatcaa tcttaacgat aacccctacc agaccgtaga
    40201 cgcccacggt cactaccagc gcgatgcctg aaagcaccag cacctgatta agcaacggcg
    40261 cttcggccac aatccccagc gtgatggcga cgatttcggc agacaatata aaatcggtac
    40321 gaatcgcccc ttttattttg tccttttcaa acttcagcgg atcctgcgcc gccagcttct
    40381 ccagacgctg ctggctctgc gccggatctt ctttatgttt acgcgcctcc agcatatgca
    40441 gcactttctc tactccttca aagcagagaa acgcgccacc aatcatcaac agaggcgtaa
    40501 tcgcccacgg gataaacgca ctgatgatca gcgccagcgg caccagaatc actttattaa
    40561 tcagcgatcc tttcgccacg ccccagacca cgggaagttc ccggttggcc cgcacgcctg
    40621 aaacttgttg cgcattgagc gataagtcat cccctaatac accggcggtt ttcttcgccg
    40681 ccagtttgcc catcacggag atatcgtcca gtagtgtggc gatatcatcg agcaacgtca
    40741 gtaaactact cccggccaaa agcattccct ctcattttta ttgttgaagg gtgtaagtat
    40801 gaagcaaaaa cggcagctct gatacttgct tcacaggtca acattttttt aacaatcaag
    40861 tgcaattata tctctcgcca gcagaatgat tttcacgttt actatgaacc acctttttat
    40921 ttccaccgtg agggattatg cgtttccgcc agttgttacc gctttttggc gcgctgtttg
    40981 cgttgtatat catttggggc tcaacctatt ttgtcattcg gattggcgtg gaaagctggc
    41041 ctccgttaat gatggcaggc gttcgattcc tggcagccgg tattttattg atggcatttt
    41101 tgctactgcg cggacacaag ctccccccgc tacgtccgct gctcaatgcc gcgctgattg
    41161 gcctgttatt gctggctgtc ggtaacggca tggtgacggt tgccgagcat caaaatgttc
    41221 cttccggcat cgccgccgtt gtggttgcaa ccgtgcccct ctttaccctg tgcttcagcc
    41281 gcctgtttgg cattaaaacg cgcaaactgg aatgggtggg tatcgccatt gggcttgccg
    41341 gaatcatcat gctcaatagc ggtggaaatt taagcggcaa tccgtggggc gcgattctga
    41401 ttttaatcgg ctcgattagc tgggcgtttg gctcggttta tggctcgcgc attaccttac
    41461 ctgtagggat gatggcgggt gcgattgaga tgctggcggc aggcgtggtg ttaatgatcg
    41521 cgtcgatgat tgcaggtgaa aaactgacgg cgctcccttc cctttcaggc ttccttgcgg
    41581 tcggctatct ggcgctgttt ggttcgatta tcgccatcaa cgcttatatg tatttaatcc
    41641 gtaatgtcag tccggctctc gccaccagct acgcctacgt taacccggtg gtcgcggtct
    41701 tgctgggtac gggactgggc ggagagacgc tgtcgaagat tgaatggctg gcgctcggcg
    41761 taattgtctt cgcggtggta ctggtcacgt tgggaaaata tctcttcccg gcaaaacccg
    41821 tagttgcgcc agttattcag gacgcatcaa gcgagtaaat gaatcccctg cgtgtcgatc
    41881 tgcgcgctgg caccttcgcc gcagatccac tcttccagac gctcggtaag cgcttcatcc
    41941 gtcagcttct cgcgcccacg taatgcacac tcccagacaa tcagtacgcg ccagccgagt
    42001 tcctgcaaac gactgatatc gcggcgatcg cgttcaacat ttttacctat cttctccagc
    42061 cagaactcgg ttcgagtcgc aggcacttta aacagatagc agtgatgatg atgccagaag
    42121 cagccatggg taaatatcac gcagcgatat tcatcaacga caaaatccgg gcttccgggc
    42181 agactggcgt cctgaacgcg aaacgccagg ccctgcccgg ttaacagact ggcgaggcgc
    42241 ttctcaatcg ccgtatcacg cgtggcaatc gcgcgcatat ttttgctgcg agtggcctta
    42301 tcgtgaacgt cggccatgtt gtgcctcttg ctgacgcaac gccaccgcct gtttgatttt
    42361 tggctcaagc agttttgcca ccgcggcaaa gaccggcacg accaccgagt taccgaactg
    42421 gcgataggcc tgagtgtccg aaaccggaat gcggaatttc gcttctcccg gcgcttcaaa
    42481 acccattaag cgcgcgcatt cccgaggcgt taaccgacgt ggacgatgtt gctgattcag
    42541 cggatcgtca aagtctttct cacctttggc catatcccag ccgcgatcga ttaaaatttc
    42601 cgcgccatct ttgtaataac gcgcagacag cgtacgcgtg acgctttgcg gattgttcgg
    42661 ataaaccatt ccataaccga agccgttacc gcgcgcctga tgttttttcg catatcgata
    42721 gaggtacttc cacagcaccg gcgtcaggat atatttcgcc tcgaccatcg ggtccaacag
    42781 ctgcgccagc gtcactcgct gtgcagggaa acattcgctg atatcacgca gagtaaaatc
    42841 ggctttcaga ttaagatcgc gacgaaaacc caccagcacg atgcgttcac ggtgctgcgg
    42901 cagaaaatgt ttaccatcga tgattttcgg atcgtccggc ccgttatctt ctgcatcagc
    42961 cacgtcatag cccagttcgt ccagcgtctg catgatgatg cggaacgttt taccctggtc
    43021 gtgacttttc aggtttttga cgttttcgag cacaaacatc gccggacgac gcgcgtcgat
    43081 aatgcgtacc acatcaaaaa acagcgtgcc ctgggtatcg caggcaaaac cgtgcgcccg
    43141 cccgagcgag ttctttttcg atacgccagc cagcgaaaat ggctgacaag ggaaaccggc
    43201 cagtaaaaca tcgtgttcag gaatgtgttg acgaatatgt tccgccgccg cctcatcact
    43261 cacgccttct ttatggctga gggtgatatc gcggatatct tcattaaaat gatgcgtcgc
    43321 cggatcgcaa taatggttgg ctttataagt gcgtaccgca tgtttgttcc attcgctggt
    43381 aaacacgcat tgtccgccaa tcgattcaaa accgcgacgg atgccgccaa ttccggcaaa
    43441 tagatcgata aagcgaaacg cataatgcgg atgatgtgcc ggtggtttgg gtaacaacgt
    43501 ttgcagatgg gcgaactctt tctcacttaa acggtgccat gccgagtcat tcgccagcgc
    43561 acgttttaaa attgccgcgc tccagtgatt ctcacccaca ccattaagct gcgccaccag
    43621 cgttttaaca tcataaattt gcagcagttt ctccagcatt gcctgtgtgg cattcccggt
    43681 gctgtatgaa tcggttactg atatattttc ctgcatagat ttaaccggcc atctgagatg
    43741 gccgacaggt taacataatt acagccattt agggccacgc gcttatcccg cgggtgaaaa
    43801 cgcatctatc accttgtgat cgacaccttc attctccccc gccagttcgg cactgagctt
    43861 cgccataaac tcgactaaaa aatgcgcgtt gtgctgcgcc agctgcttgc ctcttgcggt
    43921 ttgcatggtt tgcggtagct tcagcaactt agtctggaaa tgatccagcg cgtagcgttt
    43981 atcatcaagc gggcgatgct gtgcgaacgg atcttcacca tcaaacagcg ccacgcccaa
    44041 tgcccctgaa acggcaaaca cgcgcgccag gccaatcgcc cccagggctt ccagccgatc
    44101 cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg
    44161 agcggcaatg gcatgacaaa cggcctcgat tttctccgcc ggaaattgct cgaactcttc
    44221 acggagcagg cgacgcgtct cttctgccgc caggattgaa gaacgctgcc tttgtggatg
    44281 atttttcgcc aggctcacaa tatcgtgaaa ataacaggcg gttaaaatca ccagcatatc
    44341 gacatcgtca tctgccgcca gcttctgtgc cgtagcccag acgcggcgaa aatgacacac
    44401 gtcatgggcc gcgtcctggt gctgatgatg gtttttcaac cagttttcga actgtgcctg
    44461 ccagtgttgt aagtccatac gcgctccgtt gccaaagagt cgccacctta gcagttttta
    44521 ttcatagcgt ctgctacgtg cgggacgagg tcgggtgtac caggcaatgc tgcctaacaa
    44581 tgccccgacc agagccataa ataaaaatcc gaccagtgcc cctaaccact ttcccgcggt
    44641 agagcctaaa tcgctttcaa atcccgacac cggggcatca gggatccggc tcatcaccca
    44701 gatgtaacgc gccattgccc agacgaaata gccacaccat gtatagaaag cccacaaagc
    44761 caatttgccg ccagggctgc gagagagtct ctcttccatc tttgtcgaac cgtattccat
    44821 tatgagataa cccggaaaat tcagacttag tactattatg tgataaaagt cacatttttc
    44881 cacattggat gagccagtca agactcaatc attttttgaa caaatagata aattttgcgc
    44941 aacggcaata attactggaa aaacagcaat aaagatttat ttatatcctt ggtaatagtt
    45001 ttattaattc acccaaacaa tatattagca ctgaatacaa ttataccttt tttgatttct
    45061 gtgtcatgat aaccccgtca ttaacctttt atcgtcttgt ttatattttt tgggccggca
    45121 tgatgccggc tttttttata ccttcattaa tgtgcgcgtg atcacgcctg ccgtttggcg
    45181 caacaatcat tgatactccc tatctttcca ttcaactcat aacatattga tatacattta
    45241 taattttcat gaatgtttat atttagaatt cataattctg aattatatta aatagattag
    45301 aaacattttg aaatctttta aatacatttg ttacat
//
LOCUS       DAZGXZ010000021        44393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000021 DAZGXZ010000000
VERSION     DAZGXZ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.021"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..416)
                     /locus_tag="ACHKJF_001336"
     CDS             complement(<1..416)
                     /locus_tag="ACHKJF_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948721.1"
                     /translation="MAGGIASLKSLIQSYTAGVLCEGVPACVSPWPFFLWDEEEMTKT
                     SKLDALRAATSREDLAKILDVKLVFLTNVLYRIGSDNQYTQFTIPKKGKGVRTISAPT
                     DRLKDIQRRICDLLSDCRDEIFAIRKISNNYSFGFE"
     gene            complement(467..661)
                     /locus_tag="ACHKJF_001337"
     CDS             complement(467..661)
                     /locus_tag="ACHKJF_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012440316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948722.1"
                     /translation="MKSHDRLRIVFAQIRQNWRAFAHVMHVHENHCIKRAGVAGIRAR
                     AITENSQNTSGKQKVEVICS"
     gene            933..1268
                     /locus_tag="ACHKJF_001338"
     CDS             933..1268
                     /locus_tag="ACHKJF_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948723.1"
                     /translation="MNNIPPIPQLGIYVSKIDPTLRITVTDVDIVDGEDDSPDDELFY
                     LVHWIEGEDESDMTAMGFELDPVEWQAFVESEQLVFERDPYMDSIPENSNLAKIRDFL
                     MKTKQNDHS"
     gene            complement(1461..1694)
                     /locus_tag="ACHKJF_001339"
     CDS             complement(1461..1694)
                     /locus_tag="ACHKJF_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0948724.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(1705..1893)
                     /locus_tag="ACHKJF_001340"
     CDS             complement(1705..1893)
                     /locus_tag="ACHKJF_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948725.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYNRSMDVIS"
     gene            complement(2046..4460)
                     /locus_tag="ACHKJF_001341"
     CDS             complement(2046..4460)
                     /locus_tag="ACHKJF_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0948726.1"
                     /translation="MSHDDMSNSSGFNEAAAAFSWNGPKKAINPYLDPAEVAPESALL
                     NLITLYAADNEQEQLRRDALSEQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPDMVILADVSAQPTHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLDCVRD
                     SQLSASFRFMASHQGLEGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYMVGMFAAFRKAMHKTGLRWYGVRVAEPHHDGTVHWHLMCF
                     MRKKDRRAITALLRKFAIRVDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPILDDPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAVADQGACAPWTRGNNCP
                     LAENLNQQGKDKSADGDSRTDITRMNDKELHDYLHSMSKKERRELAARLRQVKPKRRK
                     DYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLKEDD
                     KWRNLY"
     gene            complement(4457..5314)
                     /locus_tag="ACHKJF_001342"
     CDS             complement(4457..5314)
                     /locus_tag="ACHKJF_001342"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP0948727.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PSYLVADINPDLINLYKKVAADCEAFISRARALFEEANREVAYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEYRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVSAGESKSATEIIAVSGARCWVGFDPSCGVD
                     SSAVYEVRV"
     gene            complement(5311..5613)
                     /locus_tag="ACHKJF_001343"
     CDS             complement(5311..5613)
                     /locus_tag="ACHKJF_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3850 domain-containing protein"
                     /protein_id="HFP0948728.1"
                     /translation="MTRAVRIHQLKIAPNYFNAVVAGQKTAELRKDDRGYKVGDVLSL
                     CEWKHGVFTGREWAAVISHVLPVNDVMAVSEQWVMLSIRPLTPLEALGYVIAGGAV"
     gene            complement(5610..5837)
                     /locus_tag="ACHKJF_001344"
     CDS             complement(5610..5837)
                     /locus_tag="ACHKJF_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0948729.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            complement(5837..6070)
                     /locus_tag="ACHKJF_001345"
     CDS             complement(5837..6070)
                     /locus_tag="ACHKJF_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFP0948730.1"
                     /translation="MRNIEILTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            complement(6138..6479)
                     /locus_tag="ACHKJF_001346"
     CDS             complement(6138..6479)
                     /locus_tag="ACHKJF_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HFP0948731.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            complement(6443..6643)
                     /locus_tag="ACHKJF_001347"
     CDS             complement(6443..6643)
                     /locus_tag="ACHKJF_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="HFP0948732.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRLLFH"
     gene            complement(6651..7160)
                     /locus_tag="ACHKJF_001348"
     CDS             complement(6651..7160)
                     /locus_tag="ACHKJF_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HFP0948733.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PSQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            complement(7225..7401)
                     /locus_tag="ACHKJF_001349"
     CDS             complement(7225..7401)
                     /locus_tag="ACHKJF_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP0948734.1"
                     /translation="MPNLLPNQRYSELTGLSIDTINDMLADGRLPRHRLRKDKKREKV
                     MINLAALTVDALSA"
     gene            7413..7886
                     /locus_tag="ACHKJF_001350"
     CDS             7413..7886
                     /locus_tag="ACHKJF_001350"
                     /inference="COORDINATES: protein motif:HMM:NF019126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0948735.1"
                     /translation="MPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRG
                     EFIRGWDDGRGIDTGRALLNWQPHTILDHAHYMELWTGDGLAAGSAREGVNPGILATY
                     GDGGIVKTDEPGLKVPSSLRAISSRSVKRYGEISENVGTETRPRNIAFNYIVRAA"
     gene            7886..8488
                     /gene="tfaE"
                     /locus_tag="ACHKJF_001351"
     CDS             7886..8488
                     /gene="tfaE"
                     /locus_tag="ACHKJF_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0948736.1"
                     /translation="MNKAVLNSELIAIKAGDITVYNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSVDLNSWEYVPDHRGEVVYSTETGDTKEITVPGDYPEN
                     TTTIAPLTPYDEWDGEKWVTDTEAQHSAAVGAAEAQRQSLIDAAMASISLIQLKLQAG
                     RQLTQAEITRLNAVLDYIDTVTATDTSTAPDVIWPELPEA"
     gene            complement(8460..8903)
                     /locus_tag="ACHKJF_001352"
     CDS             complement(8460..8903)
                     /locus_tag="ACHKJF_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFP0948737.1"
                     /translation="MNSVFFSPGSKSFYLQELFPEYEDAGTLPDDVIEITRETYEQFL
                     GLHPEGKEIGADSSGRPVWINSPPPSKEDEVLTAEMKKISLVSEVNTYINTHQWPGKA
                     AIGRLKSEELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(8906..9400)
                     /locus_tag="ACHKJF_001353"
     CDS             complement(8906..9400)
                     /locus_tag="ACHKJF_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948738.1"
                     /translation="MMPVSHYLCIFINVGLGEAAKRNVGNGENQIPDMSFWTVTGGNG
                     NFVIRQPDGLIIQMVTVSISGPVAMNGMTDNAYAITGSNKSYIATATLPFVFPNKVLG
                     VIPLVSTTAYGGVSSNITGSYATAVCSFAAVRGNNTIVFKVDKPLNAAFPSDTSVSAL
                     IIGR"
     gene            9428..9994
                     /locus_tag="ACHKJF_001354"
     CDS             9428..9994
                     /locus_tag="ACHKJF_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0948739.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTMAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANKSNESP"
     gene            10137..11309
                     /locus_tag="ACHKJF_001355"
     CDS             10137..11309
                     /locus_tag="ACHKJF_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFP0948740.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTIVVRVPQGETEDETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            11319..11834
                     /locus_tag="ACHKJF_001356"
     CDS             11319..11834
                     /locus_tag="ACHKJF_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0948741.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            11889..12191
                     /locus_tag="ACHKJF_001357"
     CDS             11889..12191
                     /locus_tag="ACHKJF_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP0948742.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            12206..12325
                     /locus_tag="ACHKJF_001358"
     CDS             12206..12325
                     /locus_tag="ACHKJF_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0948743.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            12318..15395
                     /locus_tag="ACHKJF_001359"
     CDS             12318..15395
                     /locus_tag="ACHKJF_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0948744.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKASAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLSSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAALRGGLAAAGTVLRVLASGPLAMLRVALYAISGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPISAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGDFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHLLALPAKGGEAMVSRAATVPPI
                     YRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            15392..15877
                     /locus_tag="ACHKJF_001360"
     CDS             15392..15877
                     /locus_tag="ACHKJF_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0948745.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDIFGDLSAQLNNLQDTATSALSDISKTVGGLL
                     S"
     gene            15874..16974
                     /locus_tag="ACHKJF_001361"
     CDS             15874..16974
                     /locus_tag="ACHKJF_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0948746.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAVRHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKNESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            17065..17283
                     /locus_tag="ACHKJF_001362"
     CDS             17065..17283
                     /locus_tag="ACHKJF_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0948747.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(17519..19204)
                     /gene="ybjL"
                     /locus_tag="ACHKJF_001363"
     CDS             complement(17519..19204)
                     /gene="ybjL"
                     /locus_tag="ACHKJF_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP0948748.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            19474..19851
                     /gene="ybjM"
                     /locus_tag="ACHKJF_001364"
     CDS             19474..19851
                     /gene="ybjM"
                     /locus_tag="ACHKJF_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP0948749.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(19881..20138)
                     /gene="grxA"
                     /locus_tag="ACHKJF_001365"
     CDS             complement(19881..20138)
                     /gene="grxA"
                     /locus_tag="ACHKJF_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP0948750.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            20298..20585
                     /gene="ybjC"
                     /locus_tag="ACHKJF_001366"
     CDS             20298..20585
                     /gene="ybjC"
                     /locus_tag="ACHKJF_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP0948751.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            20569..21291
                     /gene="nfsA"
                     /locus_tag="ACHKJF_001367"
     CDS             20569..21291
                     /gene="nfsA"
                     /locus_tag="ACHKJF_001367"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP0948752.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            21352..22254
                     /gene="rimK"
                     /locus_tag="ACHKJF_001368"
     CDS             21352..22254
                     /gene="rimK"
                     /locus_tag="ACHKJF_001368"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP0948753.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            22342..22818
                     /gene="ybjN"
                     /locus_tag="ACHKJF_001369"
     CDS             22342..22818
                     /gene="ybjN"
                     /locus_tag="ACHKJF_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP0948754.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            23170..24282
                     /gene="potF"
                     /locus_tag="ACHKJF_001370"
     CDS             23170..24282
                     /gene="potF"
                     /locus_tag="ACHKJF_001370"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP0948755.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            24377..25510
                     /gene="potG"
                     /locus_tag="ACHKJF_001371"
     CDS             24377..25510
                     /gene="potG"
                     /locus_tag="ACHKJF_001371"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP0948756.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            25520..26473
                     /gene="potH"
                     /locus_tag="ACHKJF_001372"
     CDS             25520..26473
                     /gene="potH"
                     /locus_tag="ACHKJF_001372"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP0948757.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTLCCLLICYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            26470..27315
                     /gene="potI"
                     /locus_tag="ACHKJF_001373"
     CDS             26470..27315
                     /gene="potI"
                     /locus_tag="ACHKJF_001373"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP0948758.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            27375..27863
                     /gene="ybjO"
                     /locus_tag="ACHKJF_001374"
     CDS             27375..27863
                     /gene="ybjO"
                     /locus_tag="ACHKJF_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP0948759.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            27904..29031
                     /gene="rlmC"
                     /locus_tag="ACHKJF_001375"
     CDS             27904..29031
                     /gene="rlmC"
                     /locus_tag="ACHKJF_001375"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP0948760.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(29206..29937)
                     /gene="artJ"
                     /locus_tag="ACHKJF_001376"
     CDS             complement(29206..29937)
                     /gene="artJ"
                     /locus_tag="ACHKJF_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP0948761.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(30229..30897)
                     /gene="artM"
                     /locus_tag="ACHKJF_001377"
     CDS             complement(30229..30897)
                     /gene="artM"
                     /locus_tag="ACHKJF_001377"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP0948762.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(30897..31613)
                     /gene="artQ"
                     /locus_tag="ACHKJF_001378"
     CDS             complement(30897..31613)
                     /gene="artQ"
                     /locus_tag="ACHKJF_001378"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP0948763.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(31620..32351)
                     /gene="artI"
                     /locus_tag="ACHKJF_001379"
     CDS             complement(31620..32351)
                     /gene="artI"
                     /locus_tag="ACHKJF_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP0948764.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(32369..33097)
                     /gene="artP"
                     /locus_tag="ACHKJF_001380"
     CDS             complement(32369..33097)
                     /gene="artP"
                     /locus_tag="ACHKJF_001380"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP0948765.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(33315..33830)
                     /gene="ybjP"
                     /locus_tag="ACHKJF_001381"
     CDS             complement(33315..33830)
                     /gene="ybjP"
                     /locus_tag="ACHKJF_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0948766.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            33956..34279
                     /gene="ybjQ"
                     /locus_tag="ACHKJF_001382"
     CDS             33956..34279
                     /gene="ybjQ"
                     /locus_tag="ACHKJF_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP0948767.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            34276..35106
                     /gene="amiD"
                     /locus_tag="ACHKJF_001383"
     CDS             34276..35106
                     /gene="amiD"
                     /locus_tag="ACHKJF_001383"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP0948768.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(35103..36116)
                     /gene="ybjS"
                     /locus_tag="ACHKJF_001384"
     CDS             complement(35103..36116)
                     /gene="ybjS"
                     /locus_tag="ACHKJF_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0948769.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(36215..37645)
                     /gene="ybjT"
                     /locus_tag="ACHKJF_001385"
     CDS             complement(36215..37645)
                     /gene="ybjT"
                     /locus_tag="ACHKJF_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP0948770.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(37656..38657)
                     /gene="ltaE"
                     /locus_tag="ACHKJF_001386"
     CDS             complement(37656..38657)
                     /gene="ltaE"
                     /locus_tag="ACHKJF_001386"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP0948771.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQTAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(38694..40412)
                     /gene="poxB"
                     /locus_tag="ACHKJF_001387"
     CDS             complement(38694..40412)
                     /gene="poxB"
                     /locus_tag="ACHKJF_001387"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP0948772.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(40545..41513)
                     /gene="hcr"
                     /locus_tag="ACHKJF_001388"
     CDS             complement(40545..41513)
                     /gene="hcr"
                     /locus_tag="ACHKJF_001388"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP0948773.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(41525..43177)
                     /gene="hcp"
                     /locus_tag="ACHKJF_001389"
                     /gene_synonym="priS"
     CDS             complement(41525..43177)
                     /gene="hcp"
                     /locus_tag="ACHKJF_001389"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP0948774.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(43321..44220)
                     /gene="lysO"
                     /locus_tag="ACHKJF_001390"
     CDS             complement(43321..44220)
                     /gene="lysO"
                     /locus_tag="ACHKJF_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP0948775.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT        10828 a        11271 c        11628 g        10666 t
ORIGIN      
        1 ctctcaaaac caaaggaata gttgttacta attttcctta tagcaaagat ctcatctcta
       61 caatcagaaa gtaagtcaca tattcttcgt tggatgtcct tcaaccggtc tgtaggtgca
      121 gaaatagtcc ttaccccttt tcctttcttc ggtattgtaa attgagtgta ttgattatcc
      181 gagccgattc tatatagaac gttagttaaa aataccaact taacatctaa aattttagcc
      241 aagtcttcac gtgaagtagc agccctaagt gcgtcaagtt tagatgtttt tgtcatttct
      301 tcttcatccc agaggaaaaa aggccaagga gaaacgcatg caggcactcc ttcgcacagc
      361 acacctgccg tatagctctg aatcaaggat tttagggagg cgattcctcc tgccattaag
      421 gccactggtt gcgattcaca accaacaaat ctgcctacat gcgtgtttat gaacatatca
      481 cttcaacttt ctgttttcca gaagtatttt ggctattttc ggtgatagcg cgcgctcgta
      541 tccccgccac gcctgcccgc tttatgcagt ggttttcatg cacatgcatg acatgagcaa
      601 aagcccgcca gttctggcgg atctgagcaa agacgatcct caatcgatca tgcgatttca
      661 tgcagcatag tcatgcactg tcaaggaagt gaaaatccgt atctgaatgg ccacttgaaa
      721 aacggatcat acgggtttac aaagatgaat gttcgctgtg aacgggaagc ggaagttaac
      781 tttcagataa cataatccat atgcgcgaga acctctaaaa tagaatggga cacctaagcg
      841 gggtacttac aacgattctt tccatattca cagttaacac tctgtttggc gtatttttat
      901 tcaaatagca aacaccaata aaaggagttt ccatgaacaa tattccccct ataccacagt
      961 taggaattta tgtctcaaaa atcgatccca ccctacgtat cactgtaacc gatgttgata
     1021 ttgttgatgg tgaggatgat tctcctgatg atgaattgtt ttatttagtc cactggatcg
     1081 agggggaaga tgaaagtgat atgacagcaa tgggatttga gctagaccca gtagagtggc
     1141 aggctttcgt tgaatctgag caattagtgt ttgagcgtga tccgtacatg gattcaatcc
     1201 ccgaaaattc aaacttggca aagattcggg attttctcat gaagactaaa cagaatgatc
     1261 attcgtaagt gtaagcatcc atcaggaaaa tggttttgta agtgaatcat caacttttag
     1321 agagtctcag acactcccac ttctgcttct gacacaaagc ggacgatcac ttatcaaaat
     1381 aaccgcccac cttacgcctt atttcactca ttgcccaaac tagcccccat cagaatgaat
     1441 cctcctgggg gcaacatttc ttaatgcagc cagctgtcgt cctcccacac cttctgcata
     1501 attttcatca cttgttttct ttcttcatcc agttgcagtc cggttagttc cacaccgtta
     1561 gagctacctt tgcgaatgcg aattaccgtt ttgggataca gggggcgcag attgcggtaa
     1621 agctcggatt caagtgcgtc caaggtagac tggctaatct tctgctcttt atcgatcatt
     1681 atttcaatgc gcataaaagt cacctcagct gatgacatcc attgagcggt tgtattcgtg
     1741 gcttctgatt tttgccatga gttcatcagt caattcagaa acccactgca gagccagccc
     1801 cttctcttca tcactacact cactagccgc tacaagctta agaaaaaaat caatgcgctg
     1861 gagcttcaaa gactccaaaa aatagtcctg catctttcct cctatgacac caaaacaata
     1921 ctgtatacat aaccactgtt tatatttaca gtatataata atcttactga tgtaaaacgt
     1981 ttttttacgt tcatcggcct gatatgcctg gtattattaa gagcacgaat tgttaacccg
     2041 cgtaattaat acaggttccg ccacttatca tcttccttca gacgctggtt ccgatagaag
     2101 atacgcaggc ctgctcctga cggaatactg ccgccgcgaa ggagcaaatc gacttctttc
     2161 tcgctgccat caaatcccct ggacttcagt tcatagacga gctgctgtcg ctgatggtct
     2221 gtaattcgct gtttgtagtc tttacgccgt ttcggtttca cctggcgtaa ccttgcagcc
     2281 agttcccggc gctctttttt gctcatactg tgcaggtaat cgtgcaactc cttgtcattc
     2341 atacgggtaa tatccgttct ggagtcccca tcagctgatt tgtctttccc ttgttggttc
     2401 aaattttcag caaggggaca gttattgcca cgagtccaag gggcgcaagc gccctggtcg
     2461 gctaccgcct cctgaacgtc aacggcctta cgaaccattt tccacttcac tgcatgagtg
     2521 cagatcttgc cctctgcaat gggtgaccag atgccataaa tacgaatgcc gtgatcgcca
     2581 taggcggtcg gctcttcgtt gatttcataa gcggttctga taaggtgata tttacgggga
     2641 accagtacgc cgccctgctt catgatgtag gtggcaaaac aaccagcatc agcagcagcc
     2701 aggatggcat caaggcgcgg gtcatccagt atcggcgcac ctgctttttt gtcaccctgt
     2761 tgccttgccg cctgaccagc cagcaagcga agttcacggt aagcctgacg tcccggaata
     2821 ccaaagaagc ggaactgctg aacacgatgc agagacgccc aggcattaac gtattcggcg
     2881 ttatcacgca gggatttacc cgtttccttg ctgatctcgc cagccagacc acgcccgtca
     2941 atgttcttac tgatgtattt cgcgatgtag cttgtcggtg ttcctttgcg cgggttaatc
     3001 agctcagact taaagcgtgg ccccgtgttg ttgcccagct cctcgcggtc tacacggatg
     3061 gcaaacttac gcagtaatgc agtgatggcg cggcggtctt ttttacgcat gaagcacatg
     3121 agatgccagt gcacagtacc gtcatggtgc ggctcagcca cccgcacgcc ataccagcgc
     3181 aacccggttt tgtgcattgc cttacgaaat gcagcaaaca tgccgaccat ataatcgctg
     3241 ctttgtctta ccgtcgcatt tgtccaggtc gggttgggtc tgccgttgtt gagcgtggaa
     3301 tggaaacgtg acggacaggt gatagtgtag aaaacggcgc agtcaccgcg catttccgcg
     3361 ataagctcca gacctttaac acaggccatc atctcattgc ggcgatgcgc agggttgctg
     3421 ctgctggcgt ttaccacatc ctccatgtcc agcgtgtcgc cgtcttcgtt caccagttca
     3481 tgagaacgga aaaactccag cgacttacgg cgctgctcac gtttatgcat cacggcttca
     3541 tagctgacat agggagatgc ttttttgctg accaggcaga cagcacgcaa ctgctcttcc
     3601 cgccattcgc aacgcatctt ccataatttc cgataccacc agtcggcgca caacatacgc
     3661 gccagcgaac ccggaatgag ttcatagggc acgggtttac ggcggtttct tttccgacgg
     3721 agttgctcaa acgcaggtgg gatgacatcc agacgcaggg tttccgccgc caccttttcc
     3781 catgtcttgc ggatttcttc tggcttaacg tcatcggtgg catacaaatc gccacaagct
     3841 gcatcaaggc acatgctcat atgcgcagct accagggtgg acaggcgttt cacctgatcc
     3901 tgactcattt caggcagaat cagcaggccc tccagccctt gatggcttgc cataaaacgg
     3961 aaagaagcag acagctgact gtcacgtaca caatccagtc gctccagaca tggcttaatc
     4021 gtctcacgca aatagcggga ataagccttt ggcctgccca ggctgctgaa gtattcgata
     4081 cgttgcatca gcggcttgct aatatgggtg ggctgggcgc tgacgtccgc cagaatgacc
     4141 atgtccgggt taaaacgctg ctgttcatgc gctagctttg cccggctaat gagcttatcc
     4201 tgctccattt cgcgctggac aggatcacga gattcattaa agaaataacg ctcccagacc
     4261 tgctcactca gtgcatcgcg gcgcagctgt tcctgctcat tatcggcagc gtacagagtg
     4321 atcaggttta aaagtgcaga ctccggagca acttccgccg ggtccagata agggttaatg
     4381 gcctttttcg ggccgttcca tgaaaatgct gcagcggcct cgttaaagcc gctagagttg
     4441 ctcatatcgt catgactcat acacgcacct cgtacacagc agaactatct acgccacacg
     4501 aaggatcaaa tcccacccag cagcgcgccc cggaaacagc aatgatttct gttgcagatt
     4561 tactctcgcc agccgacacg ccgatgctgc gttttgcctt gatgtagtgg tgagtgaaat
     4621 ttcgatacag cgaacggatc agggatgtgt cactgttaga aacaatgact ggatgacctt
     4681 ctgatgatcg atattcaaga acagatgcca ggtgatactg gtcatcttca gtgaagccgt
     4741 cagtgtgata gccggaaaac gtaccgtcat aaggcggatc gcaatacacc acatccccca
     4801 cctgcagcat cgccagcgtt tcatcaaagc tggcgcagat aaacgttgct cgctgggctt
     4861 tctctgcaaa tgcgcgaatt tctttttcag ggaaatacgg atttttataa ttaccgtatg
     4921 gaatgttgaa atgcccgctc ttgttataac gacataaccc acggtaacca tgacgattga
     4981 gatacaggaa atataccgct ttcatgaaat cagtaatttc agtggagtaa ttaaactcct
     5041 gccttatgtt gtaataagcc acctccctgt ttgcttcctc aaataaagct ctggcacgag
     5101 atataaacgc ctcgcaatca gcagcaacct ttttatagag gttgattaaa tcaggattaa
     5161 tatccgcaac aagatagctg gggtaatccg tctccatcat cacagcacag gaacccgcga
     5221 aaggttcaac cagtcgcggg ccagcaggaa gatgtttttt cagttcggac ataatggcag
     5281 ttttattacc cgcccatttc aggatggtgc tcatacagca cctcctgcaa taacatatcc
     5341 taaagcttct aatggggtta atgggcgaat tgatagcatc acccattgtt ctgaaactgc
     5401 catgacgtca ttaaccggaa gcacatgaga gataacagcg gcccattccc tacccgtaaa
     5461 tacgccatgc ttccattcgc aaagagaaag aacatcacca actttatagc cacgatcgtc
     5521 tttacgaagt tcagccgtct tttgacctgc aaccacagcg ttgaaatagt taggtgcaat
     5581 ttttaattga tggatacgca ctgcccttgt catacagcac ctccgttgta atgtttgcct
     5641 ttcagctctg cgatttcctg gcaggtaatg caaagctgca ctcccggaat ggcacggcgg
     5701 cgtgctggcg gaattggcgc ttcacactca atgcaaagca cgcgagacac gcccggtgtt
     5761 ttggcacggg cagcacggat atggcgctgg cgttcttctt caacgcgctg ctgtacgaga
     5821 tccattgcat cagccattag tggatctcct gcgcttcgtt ctggattgct tcagcagtca
     5881 cacgcagcag ttctgccgct tccacgtggt ttagctgacg ggatgagata tgacacgcca
     5941 ggctatcaag gcgagctgcc attgcttcag cccttgcccg gcgttcttcc agacgagcct
     6001 ctgtcagtaa aatattaagc cctgcatcat ccggtccggt tttagtcgtg aggatttcaa
     6061 tattacgcat aatcaattct cctgaattta gataaaggga tgcccggcgg gtttacgcca
     6121 ttaatttcat tagttggtta attcggcatg gttagccgtc tgggaaataa gctcaccact
     6181 gcacgaaaat gattcattgc tttaatcaac tcccgctttt cgtcagtggt cagctcattg
     6241 atgctgatgc tatgacgttc agccggaatt tttgccataa agaatatggc tgccagtgct
     6301 cgtttatttt gttcgttatt gatatcccgt ggatcacgca tatctttaat aaaccgctca
     6361 agctctgact caatattcag gccaaaaact ttcgccctta attccgctat gtgattaagt
     6421 ccattcaggc gttcaccggg gcttaatgga acagtagccg cagcgccttc aatagccatt
     6481 tgttcccccg ttttttcgta gatagttctg ccagcaattc atcttgtgaa cggcacggat
     6541 gccagcgttt accatcctca cccatgatcc agccgtgacc gtagtgcatt gccgggcttt
     6601 gttttaccag cagcgatgca aatgatggtt ctttcgtcag cataagcacc tcacagcaaa
     6661 ccgaatgaag caccgaggcc agtcacggta tcaactgcac tcgccatcgc aggattagcc
     6721 tgtaaacggg cctgcaatga aacagcagcc agcgccatca gtcgtgttac agagttaatg
     6781 ctgctgatag catcacgacg accggcacta gtttttacat caccagatac cgcacctgct
     6841 gcaacacgtc caatctctgc agttgcgctc atgacgtaat gcggcagttt ctcttttgca
     6901 acctcattaa tcggtacaca tggcaggcag tgaatctgtg ccagaaaacc atctaccagc
     6961 gttgaatctt ctgtcagatc ggtaagcagc cagatttctg gtgcggttaa taaatgaggt
     7021 tgagatgggt tcagcttgtt ccgcagaatc tgcacattca tgccagcacg ttctgccagt
     7081 tgcaccagat tgtggcgcag tgcgaatgca cgacaggctt catcaaaatg tggatgtttg
     7141 gaaacttggt aatcaaacat ggttttcaac tccgaactta tcgcaaaatc gaactcagcg
     7201 tcttattgcg aaaatagaca tctattaagc agacaaagca tcaacagtca gagcagccag
     7261 gttaatcatt accttttcac gttttttgtc tttacgaaga cgatgacgag gtagtcggcc
     7321 atcagccaac atgtcgttaa tcgtatcaat agaaaggcca gtcagttcgc tataacgttg
     7381 gtttgggaga aggttcggca ttacccgttg gtgtgcctgt tccgtggcct tccgccactc
     7441 cgccaacagg ctggctgaaa tgcaacggtg cggctttttc tgctgaagaa tacccggaac
     7501 tggcaaaggc ttacccgacc aataaattgc ctgatttacg cggtgaattt attcgtggct
     7561 gggatgacgg tcgcgggatt gatactggtc gcgctttgct taattggcag ccacacacaa
     7621 ttttggacca tgcacactat atggaattat ggacagggga cggactcgcc gcaggaagtg
     7681 cacgggaagg agtaaaccca ggaatactgg ctacatacgg tgacggggga atagttaaaa
     7741 cggatgaacc cggtcttaag gtgccttcct cactacgagc tattagctct cgtagtgtta
     7801 aacgttatgg tgaaattagt gaaaatgtag gtacagaaac tcgtcctcgt aacatcgctt
     7861 ttaattatat tgtgagggcc gcatgatgaa taaagctgta ttaaatagcg aactcattgc
     7921 cataaaagcg ggagatatta ccgtttataa ttatgatggt gaaacgcggg aatatatttc
     7981 tacatcaact gaatatctcg ctgtcggcgt cggtatcccg gcatgttcct gtctggatgc
     8041 accaggtaca cataaagctg gttatgcaat ctgtcgctct gtagatttaa attcatggga
     8101 atatgtgcca gaccatcgcg gtgaagttgt ctatagtacc gaaacgggag ataccaaaga
     8161 aatcacagtt ccgggtgatt accctgaaaa tacaaccact atcgccccat taacgccata
     8221 cgatgaatgg gatggtgaga aatgggtgac agataccgag gcacagcata gcgccgcagt
     8281 aggcgcggca gaagcacagc gccagtcgct gattgatgct gctatggctt ccatcagtct
     8341 gattcagctg aaattgcagg ccgggcggca gctgacgcaa gcagaaatca cccgccttaa
     8401 cgctgtgctg gattacattg acacggtgac ggcaacagat accagcaccg cgccggatgt
     8461 catctggcct gaactgccgg aggcgtaggc cattcaatat ctggagcact ggaggtatca
     8521 accagttcca gtgcgtccag ataatccagc cacaaattat attgcgccag ttcctcactt
     8581 ttcagacgac caatcgccgc tttgccaggc cactgatggg tattgatgta ggtattgact
     8641 tcagaaacca aagatatttt tttcatttca gccgtcaaca cctcatcctc ttttgaaggg
     8701 ggtggggaat taatccatac tggccgtcct gaactgtcag cgccaatttc tttcccttct
     8761 ggatgcagcc caagaaattg ctcatatgtt tctctggtaa tttcaataac atcatcagga
     8821 agcgttcccg catcctcata ttctggaaac aattcttgca gataaaaact tttacttccg
     8881 ggtgaaaaga atactgagtt cattattacc gtccaatgat taatgctgag acgctggtat
     8941 ctgaaggaaa ggctgcattc agtggtttgt cgactttgaa cacaatcgta ttattccccc
     9001 tgacagcggc aaaagaacaa accgcagtcg cgtatgaacc tgtaatatta ctggatacac
     9061 caccataagc tgttgttgat accagaggga taacgcccag caccttatta ggaaatacaa
     9121 agggcaatgt ggctgtggca atataagact tattagaacc tgtaatggca taagcattat
     9181 cagtcattcc attcatcgcc accgggccgc ttatacttac agtaaccatc tgaatgatta
     9241 gcccgtcagg ttgacgaatc acaaaatttc cattgccacc agtaaccgtc cagaaagaca
     9301 tatcagggat ttggttttcc ccgttgccca cattccgttt tgccgcttct cccaaaccaa
     9361 cgtttatgaa aatgcagaga taatggctaa ctggcatcat ccacggtttt tattcagggg
     9421 attgatcatg cttattggct atgtacgcgt gtcaacaaat gaccagaaca ccgatttgca
     9481 acgtaatgca ctgaactgcg cgggatgtga gcggattttt gaggataaaa tcagtggcac
     9541 taagtccgac agaccggggc ttaaaaaact gctcaggaca ctatcggcag gagacactct
     9601 ggttgtctgg aagctggaca ggttggggcg cagtatgcgg catcttgtta cgctgataga
     9661 agagttgcgt cagcgtggcg tgaatttccg aagcctgact gacagtattg ataccagcac
     9721 cccaatgggc cgtttctttt ttcatgtcat gggggccctg gctgaaatgg aacgcgaact
     9781 gatagttgaa cgtaccatgg cagggctggc tgcagctcgt gccaaaggca gagtaggtgg
     9841 acgccgtcct aagttgacca ccgaacagtg ggcacagatt gggcgtttac tcgaggccgg
     9901 agaatcaaga cagcgtattg cactgatttt tgatgtaggc gtttctacca tttatagaaa
     9961 atttccggca aataagagca atgaatcccc ctgaatcagc attattttga ttatccctgc
    10021 aagtagacaa ataccgtcat tttgtgtgaa taacgacaca actgcgctta gctgtttgtc
    10081 aggcacaatc acttcaacat agggcgaagc ctaatccaat caggaggttc gccactatgg
    10141 ctcaggatta ccaccacggg gtgcgcgttg ttgaagtcaa cgaaggcacc cgatctatta
    10201 ccacggtgag caccgccatc gtgggtatgg tctgcacggg cgatgatgcc gatgcaaaaa
    10261 tgtttcctct taataaaccc gtgctgatca ctgatgtgct gactgccagc ggtaaagcgg
    10321 gtgagtcagg tactctggcc cgttcgctgg atgccatcgc tgaccaggca aaacccgtga
    10381 ccattgttgt gcgtgtgccg cagggtgaaa cggaagacga aaccacgacc aatatcatcg
    10441 gcgcagtgac tgctgaaggt aaaaaaacag gtatgaaagc cctgttatct gcccagtcac
    10501 agctcggcgt taaaccgcgc attctcggcg tgccaggcca cgacaccaag gcggtagcta
    10561 ctgagttgct aagcgtggcg caaagcctgc gtggatttgc ttacctgtca gcgtatggct
    10621 gcaagacagt gcaggaggcg atcacttacc gcgaaaactt cagccagcgc gaagggatgc
    10681 tgatctggcc tgactttact ggctgggaca cggtgctgaa tgccgaagca acggcatatg
    10741 ccaccgcccg tgcgcttggt ctgcgcgcca aaattgacga gcagaccgga tggcacaaaa
    10801 gcctgtccaa cgtgggcgtg aacggtgtca ccggaatttc tgcagatgtg ttctgggatc
    10861 tgcaggaccc ggcaaccgat gcaggtctgc tgaaccagaa cgacgtcacc acgcttgtgc
    10921 gtaaagacgg tttccgcttc tggggttccc gctgcctgag tgatgacccg ctctttgcct
    10981 tcgaaaacta cacccgcacg gcgcaggtgc tgatggacac gatggcagaa gcacacatgt
    11041 gggcggtgga taaaccgctt aacccgtcgc tggcccgcga cattatcgaa ggtatccgcg
    11101 ccaaaatgcg cagcctggtc agtcagggct atctcattgg tggtgattgc tggctggatg
    11161 agtcggtgaa cgacaaagac acgctgaaag ccggaaaact caccatcgac tacgactaca
    11221 cgccagtgcc gccacttgaa aacctgatgc tgcgtcagcg catcaccgat cagtacctgg
    11281 tgaatttcgc cagccaggtc agcgcgtaag gggacaacat ggctttacca cgcaaattaa
    11341 aacacctgaa cctgtttaac gacgggaaca actggcaggg gatcgttgag tcgctgacgc
    11401 tgccgaaatt tacccgcaaa tatgagaagt atcgcggcgg cggaatgccg ggtgcggtgg
    11461 atgtggacct ggggcttgat gacagtgcgc tggacacaga attttccatt ggtggtactg
    11521 aattgctgct gtttaaacag atgggtaaag ccacggtgga tggcatccag ctgcgcttta
    11581 ccggctctat tcagcgtgac gataccgggg aagtgcaggc cgtggagctt gtcgtgcgtg
    11641 gacgtcacaa agaagtggat tccggcgagt ggaagacggg cgaaagcaac accaccaaag
    11701 tgaccagtac caacagctac gcgaagctga ccatcaatgg tgaggtgctc tatgaagtgg
    11761 accttatcaa catggtggaa attgtggacg gtgtggacct gatggaagcg caccgcaacg
    11821 ccctcggcct ctgatatatc tgaacggcgc gggataccgc gccagaaccc aattgacagg
    11881 acagcaaaat gagcgataag cagactgaaa agaccattca actggatacc cccatcaagc
    11941 gcggtaaaac agaaatcacc gaaattgtgc tgcgtaaacc gcagtccggt gcgctgcgcg
    12001 gtacacgcct gcaggccatt atggatatgg atgtaaacgc gatgatgacc gtgatccccc
    12061 gcatctccag tccggcactg actgcacagg aaattgcaga gatggacccg gcagatctca
    12121 ctgccatgtc ggttgaggtt gtcacttttt tgttgaagaa gtcggtgctt gccggtttac
    12181 cgacagcctg acggttgacg atctggtggc agatatcgcc accatttttc actggccgcc
    12241 atccgttact gacgttatgc cgctgaccga agtgctggaa tggcggtata aagcgattca
    12301 gagaagcggg gccaacgatg agtgataaca acctgcgtct gcaggtcatt cttaatgcgg
    12361 ttgacaagct cacccgccca tttcgatctg cacaggccag ttcaagagaa ctggctgctg
    12421 ctgtcaaaaa atcccgcgat gcaataaagc agcttgatca ggccgggagc agtctggaca
    12481 gcttccgaaa gctgcaggca gaaaatcaga aattaggcga caggctgaac tatgcccgcc
    12541 agcgtgcaaa tttgctcagt caggaactgg gagcgatggg gccgccttcg caacgtcagg
    12601 ttgttgctct gggccgtcaa cggctggctg ttcagcgcct ggaagaacgc cagaaaaagc
    12661 tgcagcagca gacggcgctt gtgcgtgctg aactgtaccg ggcgggaatt tctgcgaaag
    12721 acgatgcggg agcaactgcc cgtttagccc gtgaaacatc acgttataac caggaacttt
    12781 cgaaacagga ggcgcggctg aagcgactgg gggaagctca gcgcaggatg aatgcagcgc
    12841 gtgccagtta tgcccgttcg ctggaggtgc gtgatcgtat tgcaggtgcc ggagccacca
    12901 ccacggctgc agggctggca atgggtgcgc cagtgatggc ggcagtaaaa agctatacca
    12961 gcatggaaga tgccatgaaa ggtgtggcaa agcaggtcaa tggtctgcgt gacgataatg
    13021 gcaaccgcac tgcacgtttt tatgaaatgc aggatgccat caaggctgcc agcgaacagt
    13081 tgccgatgga aaacggtgcg gtggacttcg ctgcactggt tgaaggtggt gcgcgcatga
    13141 acgtcgcaaa ccctgacgac agctgggaag atcagaaacg tgacctgctg gccttcgcca
    13201 gtacggcagc aaaggcggca acagcctttg agctgccagc ggatgaactg tcagaaagtc
    13261 tggggaaaat cgcccagctc tacaaaatcc ccacccgcaa tattgaacag ctcggtgatg
    13321 cgctgaacta tctggatgat aacgccatgt cgaaaggggc agacatcatt gatgtgatgc
    13381 aacgtctggg cggtgtggct gaccgtctgg attatcgtaa agcggcggcg ctgggctcca
    13441 ccttcctgac actgggcgct gcgccagagg ttgcagccag tgcagcaaac gcgatggtgc
    13501 gtgaattgtc cattgccacc atgcaaagca agagtttctt tgaagggatg aatctgctga
    13561 aactcaatcc tgaagtgatt gaaaagcaga tgacgaagga tgcgatggga accatccagc
    13621 gcgtgctgga gaaggtaaac gcgctgccgc aggacaagcg cctgtctgcc atgactatgt
    13681 tgtttggtaa agagtttggc gatgacgcgg cgaaactggc aaacaacctg ccggaactgc
    13741 agcgtcagtt aaaactgaca gcgggcaatg atgcgctcgg ctccatgcag aaagaatccg
    13801 acattaacaa ggattcactt tctgcgcagt ggttgctggt caaaaccgga gcgcagaaca
    13861 ccttcagcag cctgggcgaa acgctgcgcc agccgctgat ggatattctg tacacggtga
    13921 aaagcgtcac gggggcgttg cgtcgctggg tggaagctaa cccggaactg acaggcacac
    13981 tgatgaaagc atcggctgtt gtggctgcgg ttactgtcgg cctcggcacc ttagcggtgg
    14041 cgctggctgc agtgctgggg ccgctggcag tgatccgtct gggattctct gtgttgggta
    14101 tcaaaacgtt atcttccgtt acggcagcag taactcgaac cagcagcgcg ttgtcctggc
    14161 tggctggcgc gccactggca ctgctgcgac gcgggcttgc ttcatcgggc aacgccgcag
    14221 gtttacttac tgcgccgttg tcgtctttgc gccgcacggc atcactgacg ggaaatgtcc
    14281 tgaaaactgt agcaggtgcg ccggttgcac ttttgcggtc tggattatcc ggtttacgtg
    14341 ctgttgctgt gatgtttatg aatcctctgg cggcactgcg cggtggactg gccgccgcag
    14401 gcacggtgct gcgagtactg gcatctggtc cactggcgat gctgcgcgtt gccctgtatg
    14461 ccatatctgg tctgttaggt gctctgctca gtccgatagg tcttgtggtt actgcactgg
    14521 cgggcgtggc gctggttgtc tggaaatact ggcaacccat caccgcattt ctcggtggcg
    14581 tggtggaagg attcaaagcg gcggcaggtc ccatcagtgc agcgttcgaa ccgcttaagc
    14641 ccgtgttcca gtggattggc gacaaagtgc aggcgctgtg gggctggttt actgatctgc
    14701 tgacgcccgt taagtcgacc tctgccgaac tgcagagcgc agcggcaatg gggcgacgat
    14761 tcggggaggc actggcagaa gggctgaata tggtcatgca tccgctggac tccctgaaat
    14821 ccggcgtttc ctggttgctg gagaaactcg gcattgtcag taaagaggct gcaaaggcga
    14881 aactgccgga aagcgtgacg cgtcagcaac ctgcgacggt gaatgcagac ggtaaagtga
    14941 tgatgccatc gggtgatttt ccgtcatggg gatatggctt tgcggggatg tatgacagcg
    15001 gcggctatat cccgcgcggg cagtttggca tcgtcggtga aaacgggccg gaaattgtta
    15061 acggcccggc aaacgtgacc agccggagaa atacagctgc actggctgcc gttgtcgccg
    15121 gaatgatggg tgttgctgcc gcgccagcag agcttccacc gttgcatctt ttggcgcttc
    15181 ccgcaaaagg cggtgaagcg atggtgagtc gtgcagctac tgtgccgccc atttaccgga
    15241 ttgaagcacc gacgcagatc atcatccaga cacagccagg acaaagtgcg caggatattg
    15301 cgcgggaggt ggcccgccag cttgatgaac gtgaacgcag gctgaaggca aaagccagga
    15361 gtaactacag cgatcagggg ggatacgacg catgatgatg gtgctgggat tgtacgtgtt
    15421 tatgctgcgc accgtaccgt atcaggagct gcagtatcaa cgcagctggc gacatgcggc
    15481 aaacagccgg gtaaaccgac gtccgtccac gcagtttctg ggaccggaca acgacatgct
    15541 gacgctttcc ggtgttctta tgccggagat aacaggtggc aggctgtcgt tgctggcact
    15601 ggagcagatg gcagaacagg ggaaagcatg gcccctgatt gaaggcagtg gcacgattta
    15661 tggcatgtat gtgattgagg gactgaatca gactaaaacg gagtttttcc gcgacggtat
    15721 gccgcgccgg attgagttca ccctgtcgct caaacgggtg gatgaatccc tgtccgatat
    15781 attcggtgat ctcagtgcgc aactgaataa tctgcaggac acagcaacgt ctgccttaag
    15841 cgatatcagt aaaacggtgg gagggctgct gtcatgaatt tcagctctga actgcttaac
    15901 aaaggcaaca aaactcccgc attcagcatc agtattgagg gcagggatat caccactgtg
    15961 ctggataacc gcctgatgag tttgacgctg acggacaatc ggggctttga agcggaccag
    16021 cttgatctgg agctggacga cgccgacgga aaaatcgtgc tgccgcgccg tggtgcggtt
    16081 attacgctgg cgctgggctg gaaggggcag ccgcttttcc cgaaaggggc attcacggtg
    16141 gacgagattg aacacactgg cgcaccggac cgcctgacta tccgggcgcg aagtgctgat
    16201 tttcgtgaaa cgctgaatac ccgccgtgaa aagtcgtggc acaagaccac cgttggggaa
    16261 gtggtgaagg aaatagctgt gcggcacaaa ctgaagatgg cattgggtaa agacctgtca
    16321 gataaacccg tggaacatat agaccagacc aatgagagtg acggcagttt tctgatgcgg
    16381 ctggcgcgcc agtacggtgc tattgcgtcg gtgaaaaatg gcaatctgtt attcatccgg
    16441 cagggacagg gtaaaagcgc cagcggtaaa ccactaccgg ttatcactat cacacgtaag
    16501 gacggcgaca gtcaccgctt taccctggca gatcgcggag cctacacggg cgtaattgcc
    16561 agctggttgc atacccgcga acccgcgaag aatgaaagca ccacggtgaa gcgtaagcgc
    16621 aggactaaga agcagaagaa agagccggaa gcgaagcagg gcgattacct ggtgggtaca
    16681 gatgaaaacg tgctggtact taatcgcacc tatgccaacc ggagcaacgc cgaacgggcg
    16741 gcgaaaatgc agtgggaacg cctgcaacgc ggcgttgcgt cattctcgct acaactggca
    16801 gaaggtcggg cagatctcta cacggaaatg ccagtgaaag tcagtggctt taaacagccg
    16861 atagatgatg cggaatggac cattacgact ctgacgcata ccgtcagccc cgataacggt
    16921 tttacaacca gtctggagct tgaagtgagg attgatgatt tcgaaatgga atgattcttc
    16981 gcaatggaga acttttaagt ttgcaaaatg gaataatgcg gtatcattat tgtgaattta
    17041 gcaaaaatgg ggagaactcg aaaaatgatg atttgcccac tgtgtggaag tgccgcccat
    17101 actcgcagca gttttcaggt atcttcattg accaaagagc gttacaacca gtgccagaac
    17161 attaactgca gccatacttt tgttacccat gaaacttttg ttcgttcgat tgcaacgcca
    17221 aaagagtcaa atccggttca gccgcatcca atgaaatcag gacaggtggc gctctctctt
    17281 tgacgctgcc gccattttgt cgccatcgtt aaaaaacagt gtttctaaca tcatgatttt
    17341 aaacagctta aatttcaggc aacaaaaaac ccatcaacct tgaaccgaaa tggcggggtt
    17401 gatgggctcc acaaaatggg gacatcaaag aaaagcagtg gcactaatta agactgatgc
    17461 cctgcggaaa agttctgcgg ttgtgcaaaa aaatttcatt ttcagggcaa cttcagtttt
    17521 atcctaatcc tggccatacc atgacgatga ttgtccctgc cagcgtcagc aggacgttgg
    17581 cgattgcgta ggtgcccgca tagcccagcg cagggatgtt actgcgagct gtatcactga
    17641 tgatctccat tgccggcgcg caggtgcgtg cgcccatcat tgcgccgaac aacagtgcgc
    17701 ggttcattcg caatacataa gcaccgaaca agaaacagat aaccacgggc acaagactga
    17761 caattaatcc ggcaatcaac atctgaccgc caatcgcgcc caggccgtta ttaataccgc
    17821 taccggcgct cagaccaacg cctgccataa acaccatcaa gccgaactct ttcaccatgc
    17881 ttaatgcacc ttgcggaatg taaccgaagg tcgggtggtt agcacgcata aagcccagca
    17941 taattccggc gaataacaac ccggcagcgt tccccatgcc gaaactgaat gtgctgaact
    18001 ggaaggtgat catcccgatc atcagcccaa taacaaagaa ggcgcaaaat gccagcaggt
    18061 cagtgacctg gctgtgaatc gagataaagc cgatgcgatc ggcgatggtt tttacgcgac
    18121 gggcatcacc gctgacttgt aaaacgtcac ctttgttaag cacgacgttg tcatctatcg
    18181 gcatctcaat ctggctacga atgacgcggt taaggaagca accgtgatcg gtcaacttca
    18241 gttgtgcgag acgtttacct acagcgttat ggtttttaac gaccacttct tcagtgacga
    18301 tacgcatgtc gagaaggtca cgatcgaaaa cttctttacc gttacggaag ctgggatcga
    18361 gtcgggcatg ggcgtcggga tagcctacca acgctatttc atcgcccatt tgtagcacgg
    18421 catcaccgtc tggatttgcc agaatcccgt tacgtcgaat acgttcaatg tagcagccgg
    18481 tttgtcgata aatacccagt tcacgcagat ttttgccgtc ggtccaggcc accagctccg
    18541 ggccgacgcg ataggcgcgg atcaccggta aataaacctt acggttggca tcagtgtcca
    18601 ggccacgttc gcgggcgatt tgctgggcgc tggtctgtaa gtcctgatgc tgcaatttcg
    18661 gcaagtaacg cgcaccaaca atcaaactca ccagaccgat taaataggtt aaggcatacc
    18721 cgaggctcag attatccagt gccagtgaga gctgcctgct ttccatgccg gaatgacgca
    18781 gtgtatcgcc agcaccgacc agaaccggtg tcgacgtcat agagcctgct aacataccgg
    18841 ccgtcaggcc aatatcccag ccaaacagct tacctaaccc taaggcgatc accagcgcac
    18901 tgccaaccat caccagtgct aacattaggt aatttttccc atcgcgaaaa aaaatggaaa
    18961 aaaagttcgg tccggcttcg accccgacgc agaaaataaa cagcataaag ccaagattaa
    19021 gcgcatcggt gttaatgctg aaatgttgtt ggcctaataa cagcgatacg actaaaacgc
    19081 caatggaatt acccagttgg atcgaaccaa gtcgtaactt tccgagacat agcccaagcg
    19141 cgaggaccac aaataataac agaatgtaat tcccatttaa caattcggcg acgtttatat
    19201 tcacggaggc taacttcttg tttactagta agctgttgaa agaaatggta atttacgata
    19261 atgtttttta ccagaattca gggcgcagat tcattcagcg cacctaaacg atagtaaagt
    19321 aacaatatat tttactagtg taatcacatt aggtatcaac ggctatatga attgcgttgg
    19381 cctatattag catggaatgc gaagcggctt tatcttactg aacgccacac tggcgaaaaa
    19441 tgtgttcgat agacgcagtg tcaggaggaa cgagtgaaac ataaacaacg ttgggcgggg
    19501 gcaatctgct gttttgtcct cttcattgtg gtgtgccttt ttctggcgac gcacatgaaa
    19561 ggcgcttttc gggctgccgg gcatcctgaa atcggcttgc tatttttcat tcttcctgga
    19621 gcagttgcca gtttcttttc acagcgtaga gaagtcctga aacctctatt tggcgcaatg
    19681 ctggcggcac cctgttcaat gctcattatg cggctgtttt tttcaccgac gcgctcattc
    19741 tggcaagagc tggcatggtt actaagcgcg gtgttctggt gtgcgctggg ggcactgtgt
    19801 ttcttattta tcagtagttt gtttaaacca cagcacagaa aaaatcagta aagccctcaa
    19861 cgcgagggct tgtcagacga tcaggcgtcc agattttctt tcacccatgc agcaaaatcg
    19921 gtatagccgc cgatatgttg ctgatcgaca aaaatctgcg gcacggtttc tacgggttta
    19981 cctgcctttt gttgtagatc ttctttagtg atcccttccg cacgaatatc tacatactga
    20041 tactgaaaat catcgcgttc attgctcaat ttctcagcca gatcttttgc acgcacacag
    20101 taagggcaac ccgaacgacc aaaaataacg gtttgcatta tttctctcct catagattta
    20161 tgcctgtaat gatcacgcta aaatgtattc gctgaaagta ggtttaacct gttgcattaa
    20221 ttgctaaaag ctataactgt taaacacaat acagtgaaaa gttttagact gaaggctcac
    20281 tttgcagagg gaagcgtatg cgcgcgatcg gtaaattgcc taaaggcgtg ttgatactgg
    20341 aatttatcgg aatgatgcta ctggcggtgg cgctgctgtc ggtaagcgac tccctgtcgc
    20401 tgcctgagcc attttctcgg ccagaagtgc agattctgat gatttttctc ggtgttttgc
    20461 tcatgcttcc cgctgcggtg gtggttattc ttcaggtggc aaaacgtctt gccccacagc
    20521 tgatgaaccg tccaccgcaa tattcacgtt cagaaagaga aaaagataat gacgccaacc
    20581 attgaactta tttgtggcca tcgctccatt cgccatttca ctgatgaacc catttccgaa
    20641 gcgcagcgtg aggcgattat taacagcgcc cgtgcgacgt ccagttccag ttttttgcag
    20701 tgcagtagca ttattcgcat taccgacaaa gcgttacgtg aagaactggt gacgctgacc
    20761 ggcgggcaaa aacacgtagc gcaagcggcg gagttctggg tgttctgtgc cgactttaac
    20821 cgccatttac agatctgtcc ggatgctcag ctcggcctgg cggaacaact gttgctcggt
    20881 gtcgttgata cggcaatgat ggcgcagaat gcattaatcg cagcggaatc gctgggattg
    20941 ggcggggtat atatcggcgg cctgcgcaat aatattgaag cggtgacgaa actgcttaaa
    21001 ttaccgcagc atgttctgcc gctgtttggg ctgtgccttg gctggcctgc ggataatccg
    21061 gatcttaagc cgcgtttacc ggcctccatt ttggtgcatg aaaacagcta tcaaccgctg
    21121 gataaaggcg cactggcgca gtatgacgag caactggcgg aatattacct cacccgtggc
    21181 agcaataatc gccgggatac ctggagcgat catatccgcc gaacaatcat taaagaaagc
    21241 cgcccattta ttctggatta tttgcacaaa cagggttggg cgacgcgcta aaaccgccac
    21301 gtcgatgtat gatacgcggg cttttgacca ggtctgacag agaggtgcag ggtgaaaatt
    21361 gccatattgt cccgggatgg aacgctctat tcgtgtaagc ggctgcgtga agccgctata
    21421 cagcgcggtc acctggttga aattcttgat ccgctttctt gctacatgaa cataaatcct
    21481 gcggcgtctt ctattcacta caaaggccgc aagttacccc attttgacgc agtgatcccg
    21541 cgtattggca ccgccattac cttttatggg acggcggcac tgcgccagtt cgagatgctg
    21601 gggagctatc cgctcaatga gtcggtcgcc attgcccggg cgcgtgacaa attgcgttcc
    21661 atgcaactgc tggcgcgtca gggcatcgac ctgcctgtca cgggcattgc gcattcgccg
    21721 gatgatacca gcgatttaat cgacatggtc ggtggtgcgc cgctggtggt caagttggtt
    21781 gaaggcacgc agggaattgg cgtcgtgctg gcggagacgc gtcaggcggc ggaaagcgtg
    21841 attgacgctt tccgcggtct gaacgcgcat attctggtgc aggaatatat caaagaggcg
    21901 caagggtgcg atatccgctg tctggttgtt ggcgatgaag tggtcgctgc gattgaacgg
    21961 cgggcgaaag agggcgattt tcgttccaat ttgcatcgtg gcggcgcggc aagcgtcgcc
    22021 agtatcacac cacaggagcg tgaaatcgcg ataaaagccg cgcgaacgat ggcgctggac
    22081 gttgctggtg tggatattct gcgtgctaat cgcgggccgt tggtgatgga ggtgaatgcg
    22141 tcgccggggc tggaaggaat agaaaaaacc accggtatcg acatcgcggg taaaatgatc
    22201 cgctggatcg aacgccacgc tacgacagaa tattgcctga aaacgggtgg ttagtcgcaa
    22261 tcacattact gatcatggtt ttgcctgcgc tttttgcgta agctgtgccg gtctttttat
    22321 cgaaagaggt tgtacaaaat tatgacatcg ctggtcgttc ctggtctgga tacgctgcgt
    22381 caatggctcg atgacctggg gatgagtttt tttgaatgtg ataactgtca ggctctgcat
    22441 ctgccccata tgcagaattt cgacggtgtc tttgatgcca aaatcgatct gatcgataac
    22501 acgatcctgt tttctgccat ggcggaagtc cgaccttcag ccgtattgcc gctggcggcg
    22561 gatttatctg ccatcaatgc cagttcgctg accgtgaaag catttcttga tatgcaggat
    22621 gataatctgc caaagctggt ggtttgccag tctttatccg ttatgcaggg cgtaacctat
    22681 gagcagtttg catggttcgt gcgtcagagc gaagagcaga tttcgatggt cattcttgaa
    22741 gctaatgccc atcaactgct gttaccgact gacgatgaag ggcaaaacaa cgttaccgaa
    22801 aactattttc tccactgata attcctttcg agcacgcagt cgctgccgca gtgactgcgc
    22861 gctgcaaaat tatctgctgt ttttaacctt ttcttaaaga ttatttcact tatcttgtgt
    22921 cgatttggct ttatcacata gagcaaatac gcataaaaat ttgttaaata ccgtttttta
    22981 atccgagcta tagtctcaaa ccctggctaa agttattctt gcgatgcttt tatatagcga
    23041 gcagtgctgg ccgggagaga gttctctttt cttacaccgc gccgataaaa aaatatgcac
    23101 gtttattgca tatctttcag tgtgacaact tttgttcgtt tgttaacgaa ctttcagaag
    23161 gaaagagata tgaccgcctt aaataaaaaa tggctatcgg gtctggttgc gggtgctctg
    23221 atggccgtct ctgtcggcac gctcgcggct gaacaaaaaa cactccacat ttataactgg
    23281 tctgattata tcgcccctga cacggtggcc aattttgaaa aagaaaccgg tattaaagtc
    23341 gtctacgatg ttttcgactc taacgaagta ctggaaggca aattaatggc gggtagcacc
    23401 ggctttgact tggtggttcc atctgccagc tttcttgaac gccagctgac agcgggagtt
    23461 ttccagccgc tggacaaaag caaattgccg gagtggaaga atctcgatcc ggagctgctg
    23521 aagttggtcg ccaaacacga tcccgacaat aaatttgcta tgccctatat gtgggcgacg
    23581 accgggattg gctataacgt tgataaagtt aaagctgtgc tgggcgaaaa cgcgcccgtc
    23641 gatagctggg acttgatcct caagcctgaa aatctggaaa aactgaaaag ctgcggtgtc
    23701 tctttcctgg atgcgccaga agaagttttt gctaccgtgc tgaattatct cggcaaagat
    23761 cccaacagca ctaaagcgga tgattacacc ggaccggcta ccgatctgct gttaaagctg
    23821 cgcccgaaca ttcgttattt ccattcatct caatacatta acgacctggc aaacggcgat
    23881 atctgcgtcg ctattggctg ggcgggcgat gtctggcagg cgtcaaaccg cgcgaaggaa
    23941 gcgaagaatg gcgttaatgt ctcgttctcg attccaaaag aaggggcgat ggcgttcttt
    24001 gatgtattcg ccatgcctgc ggatgccaaa aacaaagacg aagcctatca gttcctgaat
    24061 tacctgctgc gcccggatgt agtagcgcat atttccgacc atgtgttcta tgccaacgcc
    24121 aataaagcag ccacgccgct ggtgagtgcg gaagtccgtg agaacccggg tatttatccg
    24181 cctgcggatg ttcgtgcgaa gctgttcact ctgaaagtgc aggacccgaa aatcgaccgt
    24241 gtgcgcaccc gcgcgtggac caaagtgaag agcggaaaat aatccgcagc cttagatgcc
    24301 gcgcgggcgc accacacccg ccggcaattc gcaccatcat ggtgcgcttg cacacattca
    24361 atgccggaga gcagccgtga atgacgctat ccctcgcccg caggcgaaaa cccgtaaggc
    24421 gctgacgccg ctattagaaa tccgcaacct caccaaatcc tacgatggtc aacatgcggt
    24481 ggatgatgtc agcctgacca tctacaaagg tgaaatcttc gcgctgctgg gcgcatccgg
    24541 ctgtggcaag tccacgctgc tgcgtatgct ggcaggtttc gaacaacctt ctgccggaca
    24601 gataatgctt gatggcgtcg atttgtcaca ggtgccgcct tacctgcgcc ccatcaatat
    24661 gatgtttcag tcttacgcgc tgtttccgca tatgaccgtg gaacagaaca tcgcttttgg
    24721 cctgaaacag gacaaactac cgaaagcgga aattgccagt cgggtccatg agatgctcgg
    24781 cctggtgcac atgcaggagt tcgccaaacg caaaccgcat cagctttccg gtggtcagcg
    24841 acagcgtgtg gccctggccc gaagccttgc caaacgcccg aaactattac tgctcgatga
    24901 gccgatgggc gcgctggata aaaagctgcg tgaccggatg cagcttgaag tggtggatat
    24961 tctggagcgc gtcggtgtga cttgtgtgat ggtcacccac gatcaggaag aggcgatgac
    25021 catggcgggg cgtatcgcca ttatgaatcg cgggaaattt gtccagattg gcgagccgga
    25081 agagatctac gagcatccaa ctacccgcta cagcgctgaa tttatcggtt cggttaatgt
    25141 ctttgaaggt gtactcaaag agcgtcagga agatggcctg gtgcttgatt cgccggggct
    25201 ggtgcatcca ctgaaagtcg atgcggatgc ttcagtggtc gataacgtgc cggtacacgt
    25261 agcgctgcgc ccggaaaaaa tcatgctttg cgaagagccg cccgccaatg gttgtaactt
    25321 cgcggtgggg gaggtgatac acattgccta tctcggcgat ctttcggtgt atcacgttcg
    25381 tctgaaaagt gggcagatga ttagcgccca gctacaaaac gcccatcgtc atcgtaaagg
    25441 gttaccgacc tggggcgacg aagtgcgttt gtgctgggaa gtggacagct gtgtggtgct
    25501 gacggtttaa ggagcaaaga tgaatacact tgaacctgct gcccagtcga aaccgccggg
    25561 cggatttaag ctgtggttgt cgcagctgca aatgaagcat gggcgcaaac tggtcattgc
    25621 gttgccatat atctggttga tcttgctgtt tctgctgcca tttctgattg tctttaaaat
    25681 aagcctggcg gagatggcgc gcgctattcc accttatacc gaactgatgg agtgggctga
    25741 cgggcaactg tccatcactc ttaatctcgg taattttctg caactgactg acgatccgct
    25801 ctatttcgat gcttatctcc agtcgttaca agtggcggcg atttcgacac tttgctgttt
    25861 actgatctgc tatccgctgg cgtgggcggt ggcgcacagt aagccctcga cccgtaatat
    25921 cttattacta ctggtgatcc tgccgtcgtg gacctcgttt ctgatccgcg tttatgcctg
    25981 gatgggaata ttaaaaaaca acggtgtgct gaataatttt ctgctgtggc tgggggttat
    26041 cgatcaaccg ctgaccattc tgcataccaa tctggccgtt tatatcggca ttgtttacgc
    26101 ttacgtgccg tttatggtac tgccgattta taccgcgttg attcgtattg attattcgct
    26161 ggtggaagca gcgctggatc tcggtgcacg accgctgaaa acgttcttta ctgtgatcgt
    26221 gccgctgact aaaggtggga ttattgccgg atcgatgctg gtgtttatcc cggctgtggg
    26281 cgagtttgtg atcccggaac tgctcggtgg cccggacagc atcatgatcg ggcgcgtgct
    26341 atggcaagag tttttcaata accgcgactg gccggtggcc tcggcggtag cgatcatcat
    26401 gttgctgctg ctaattgtgc cgataatgtg gttccacaaa caccagcaaa aaagcgtggg
    26461 agaacacgga tgaataattt accggtagtt cgttcaccct ggcggattgt gattttgctg
    26521 ctgggcttta cctttctcta cgcgccaatg ctgatgctgg ttatctattc gtttaacagc
    26581 tcgaagctgg tgacggtgtg ggccggctgg tcaacgcgct ggtatggtga gttattgcgc
    26641 gatgacgcga tgatgagtgc ggttggttta agcctgacaa ttgctgcctg tgcggcaacg
    26701 gcggcggcga tcctcgggac tattgcggcg gtggtgctgg tgcgctttgg caggtttcgc
    26761 ggatcaaatg gctttgcctt tatgatcacc gcgccgctgg taatgccaga tgtcatcacg
    26821 ggcttgtcgc tgttgttatt attcgtcgcg cttgctcatg ccattggctg gcctgcggac
    26881 cgcggtatgc tcaccatctg gctggcgcat gtcacttttt gtacggctta tgtggcggtc
    26941 gttatttctt caagattgcg ggaactggat cgctcgatag aagaagcagc gatggatctc
    27001 ggtgcgacgc cgctgaaagt gtttttcgtc attacgctac cgatgatcat gccagcgatc
    27061 atttctggct ggttactggc ttttactttg tcgcttgatg atctggtgat cgccagcttt
    27121 gtttccgggc cgggagccac cacgttaccg atgctggtct tttccagcgt gcggatgggg
    27181 gtgaatccgg aaatcaacgc cctggcaaca ttgatcctcg gcgcggtcgg aattgtcgga
    27241 tttatcgcct ggtatctgat ggctcgcgca gaaaaacagc ggatacgcga catccagcgt
    27301 gcaagacgtg gctgaagaca ctaaaatttg ccaacctggc tacataatgc cgcgcatgtc
    27361 gcggcattgt tttcatggaa gacgaaacgt tgggattttt taagaaaaca tcttcatctc
    27421 atgctcgcct gaatgtgcct gcgttggtgc aggtggcggc gctcgccatt attatgatcc
    27481 gtggcctcga cgtgctgatg attttcaata cgctgggcgt gcgcggtatt ggcgagttca
    27541 ttcatcgcag cgtacaaacc tggagtttaa cgctggtctt tttaagcagt ctggtgctgg
    27601 ttttcattga gatctggtgt gcgttttcac tggtgaaagg gcgtcgctgg gcgcgctggc
    27661 tatatctgct gacacaaatc accgccgcaa gttacttgtg ggcggcttcg ctggggtacg
    27721 gttatccgga gctgttcagc attcccggtg aatcaaaacg tgaaatcttc catagcctga
    27781 tgctgcagaa gctgccggat atgctcatcc tgatgctgct gttcgttccc tcgaccagtc
    27841 ggcggttctt ccagttgcaa taatgtgtat aatcgtcgcc cctgatgatg tgaaggtcaa
    27901 tgtatgcagt gcgcacttta cgacgcgggt cgctgtcgtt cctgtcagtg gataacgcag
    27961 ccgattccag agcaactctc cgctaaaacc gccgatctta aaaatctgct cgccgacttt
    28021 ccggttgagg aatggtgcgc gccggtgtca ggcccggaac aagggtttcg taataaggcc
    28081 aaaatggtgg tgagtggtag cgttgaaaaa ccactgctcg gtatgctgca tcgcgacggc
    28141 acaccagaag acctttgtga ctgcccgctt tatcctgcct catttgcgcc cgtttttgcg
    28201 gcgctaaaac cgtttattgc ccgagcgggg ttaacgccct acaacgtggc gcgtaagcgt
    28261 ggcgaactga aatacattct gctgactgaa agccagagtg atggcggcat gatgctgcgc
    28321 tttgtactgc gttctgatac caagctggcg caactgcgta aggcgctgcc gtggttacac
    28381 gaacaactac cgcagctgaa agttattacc gtcaatattc agccggtaca tatggcgatt
    28441 atggaagggg agacggagat ctacctgacc gaacaacagg cactggcaga gcgttttaat
    28501 gacgtaccgc tgtggatccg tccgcaaagt ttcttccaga cgaatccggc ggtcgccagc
    28561 cagttgtacg ccaccgcgcg cgactgggta cgacagctgc cggttaaaca tatgtgggat
    28621 ctgttctgcg gtgtgggggg ctttggttta cactgcgcga cgcctgacat gcagttaacc
    28681 gggatcgaaa ttgcgccaga ggccattgcc tgtgcaaagc agtcagccgc tgaactgggt
    28741 ttaacgcgtc tgcaatttca ggcgctggac tccactcagt ttgccaccgc tcagggggat
    28801 gtgccggagc tggtgctggt taacccgccg cgccgcggca ttggtaaacc gctgtgtgat
    28861 tatctctcaa cgatggcacc gcgttttatt atctactcca gctgtaacgc ccaaaccatg
    28921 gcgaaagata tccgcgaact gccaggttac cgcatcgaac gggtacagct ttttgatatg
    28981 ttcccgcaca ccgcgcacta tgaagtgatg acgctgctgg tgaagcaata aaaaagccgc
    29041 aggtgcggct tcagattgct gatcaggtgc gctttgttca tgccggatgc ggcgtgtacg
    29101 ccttatccgg cctacaaaat catgctaaat caatacattg cagagttgat gtgggccaga
    29161 taagcgcagc gcatcaggca atcttacttt tgtcatcagt ctcaattact gcgggaacca
    29221 ctggtcattg attttctgat atgtaccgtc agctttaatt gctgccagcg cgttattcag
    29281 tttttccagc agagctttgt tatccggacg tacagcgata cccaggccgg tgccgaagta
    29341 ttgcggatcg gtcacttttt cagtcgcaac gcccagttgc ggattggttt tcagccattc
    29401 gtttaccacc gctgtgtcac caaatacccc atcaatacga ccatttttca gatcgataaa
    29461 ggcattctga taactgtcat aggagacagt tttcacttcg gggtgctgat cctgaatata
    29521 tttctggtgc gtggtgccgt tttccatccc aatacgtttg cctttcagat cggcaaacgt
    29581 tttgtaggta tcttttttgg caatcacgac ggctgagttt tcatagtagg gcgtggtaaa
    29641 cgatacctgc ttgctacgct cgggcgtgat atccataccg gagattacgg cgtcatattt
    29701 tctgaatttc agtgacggga tcaggctgtc gaacgcgtga ttagtaaaag tacattctgc
    29761 ctgcatttgt ttgcacaagg cttttgccag atcgatatca aagccgacaa tctcattatt
    29821 agcacctata gattcaaagg gtggataggt ggctgaaacg ccaaaattga ttttctctgc
    29881 ggcagaagca ccgaaagtaa aggaagcaag taaagcggca agaactaact ttttcatgat
    29941 ggaactcccg tctgtcaatc ttatgatttt tggccgtgtc tgcggcatgg gataacaatg
    30001 ccatcaagtg aatttatatg caataaatat gattaaataa tttaaatgaa ataaaaaaag
    30061 acggatacct tagtgagttg tccgtcttca ttataagaat ttatgcacta tgtaggccgg
    30121 ataaggcgtc cacgccgcat ccggcacagg caccgtgctg atgtctgatg cgacgctggc
    30181 gcgtcttatc agacctacaa acccccccgg cgaatgtacg cagctacatt aatttcgccg
    30241 ttcgaatgcc agtgctttgc gctcgatcag acgcatcatc agcgtcagca ggccgttgac
    30301 gaccaggtaa ataatccctg ccgcaccgaa caccattaca tcgtaggtgc gtccgtacaa
    30361 caactggctg tatcccatca cttccatcag cgtaatggtg taagccagag aggtactttt
    30421 gaacaccagc accacttcgt tggaataaga agagagcgag cgtttaaagg catacggcag
    30481 caggatcgcc agcgtatctt ttttgctcat tcccagggcg ctacaggact gccactgacc
    30541 ttctgggatc gcacgaattg caccgtaaaa cagctgcgtg gtatacgccg cactgttcag
    30601 cgataacgca atcagcgcac ataaccacgg ttctgacaac aaatgccaca gtgccggata
    30661 ctcctgcaaa gtcggaaact ggcccggccc gtaataaatc aggaagatct gcaccagcag
    30721 cggcgtaccg gtaaacagcg tgatataacc ccgcaccagc cacaccagca ccggcgtttt
    30781 cagcgtcagg atgatggtaa aaatcaatgc cagaatcagt gccacaatca gcgaggcaac
    30841 ggttagcgtc aggctggtgt gtagcccttt catcagttcg ggtaaatact caaacattag
    30901 ctgggcctcc gctcaaaacg tgtcgcgcgc aggtcaatgc gtttgagaat gtactgactg
    30961 agcagggtga tcaccaggta aatcgccgcc gccacaatgt accaggtaaa tggttcctgg
    31021 gtacgagtag cgatgctttt tgtttgcagc attaaatcat tcacactaat caaactgacc
    31081 agcgcggtat ctttcagcag caccagccac tggttaccga ggccaggcag cgcatgacgc
    31141 cacatctgcg gcatcaccag acggaaaaag atagccgatt tcgacagccc cagcgcctga
    31201 ccagactccc actgacccac cggcaccgct ttcaacgcgc cccgaagcgt ttgcgaggca
    31261 taggcggcat acagcagtga cagagcgatg acaccacaaa ggaacgggct aacgtcgaag
    31321 ttctcaatgt ccatctgcac tgggatctgc acgaacccaa gattgatagt gaagccatcc
    31381 gaaagcgtca gcagcagctg cgaggagcca aaatagataa acagcaccac cagaatttct
    31441 ggcaggccac gcagaatggt taccagcgct gaacctgccc acgcgacagg acgccatttt
    31501 gccgactccc ataccgcaaa gaacatcgcc agcgccagcc cgacaatcaa tgcacaaacg
    31561 gcaaggccga cggtcatccc ggcggcgctt gctaaaggaa aaaattcatt catcaggaat
    31621 tacttctgga accatttgtt gtagatggtt tcgtaagtgc catctttctt cactttttcc
    31681 agcgcagtgt tgagtttctg ctgcagctca gtgttgccct gacgtaccgc gatgccgagg
    31741 ccagtgccga agtaatcttt atcggtcact ttgtcgccca ccgccgccag tttcgggtta
    31801 tctttcagcc actcagtgac cactgcggtg tcaccgaaga cgccgtcgat acgcccgttt
    31861 tgcagatcca gttttgcgtt ctggtagctg tcatacggaa cggtagtgat ttccgggtgc
    31921 ttatccataa tgaatttctg gtgtgtcgtc ccgttctgaa cgccgacttt tttgcctttc
    31981 agctgatcaa cactggtgta tttgccttgc tgacccacaa acagggcaga gttgtcatag
    32041 tacggggtgg taaacagtac ctgcttttca cgctccggag tgatatccat gcccgccatc
    32101 actgcttcta cgcgacggaa tttcaggctt gggatcaggc tgtcaaacgc ctggttagag
    32161 aaagtacagg ttgcatcaat ctctttacac agtgcctgtg ccaggtcgac gtcaaaacca
    32221 acgatctggt tgtttgcatc aatcgattca aacggaggat aggaggcttc ggtagcaaaa
    32281 cgaatggttt cggcagctgt ggcggaaaga ctaaaacctg caattaacgc ggcaatcaga
    32341 acttttttca ttgttgttat cccgaatctt agtgagagag atagttttta aatgcttcgg
    32401 tttgcggctc ggtaaagcag ctcgcgtcgc cttgttctac gatatgacca ttttccatat
    32461 acaccactcg gctggcggtt ttacgcgcca cttcaacttc gtgggtgacg atcacctggg
    32521 taatattcgt ttctgccagc tcacgaatga tgctgacgat ttgtgccgta atttccgggt
    32581 ccagtgcggc ggtcggttca tcgaacagca gtacctgcgg ttccatcatc aacgcacggg
    32641 caatagcaac acgctgctgc tgaccaccag aaagatgcag cgggtaacga tcgctataag
    32701 gtttgagacg cagacgttcc agcagttttt ctgcacgggc cagcgcctga tctttactca
    32761 accccagtac acggcagggc gcttcaatca ggttttgctg cacggtcaga tgcggccaca
    32821 ggttgtattg ctgaaacacc atgccaacgt tacgacgcaa atcgcgaatc gctttgtcag
    32881 agggtgtttt ggtgaaatcg aaatggttgc ctgcaatgtt gagcgtaccg gagcgcggca
    32941 tctcaagcag attgagtaca cgcagcagcg agcttttacc cgcgccgctg gggccaagta
    33001 acaccagcgt ttcgccctgt gggcaatcca gcgtgatatc gaacagcgcc tgatgcgcgc
    33061 cgtagaagca attaatgccg tttaattgaa tactcattga cactcgtata ctggcagtat
    33121 gatagctatt gaggtcgaag atagtacctt tgacagaata attatgcaat atttctgctt
    33181 taaaagttaa aagcaaagcg cattattcaa taaacatagc acaaaataac gggggcggtg
    33241 gtcggtgagc ataaatgtcg gcattcctca cgaaatgccg gacaatttac ggggtttatt
    33301 ggttgatcaa ggcgttagcg attctcgatg gactgacgga gcgtacccgc tgtggcatga
    33361 acgctgccgc ctaaatagcg tacatcgtcg atcacccaac actggccttc ctgaatcatt
    33421 aacacttcat cctgccagct ctgatcaccc tgttttagat ccacacgcag aggaatattt
    33481 cgcgcatcac gattcgggat tgttgaggca ctggcaacac gggcgctctc tggcaaggtt
    33541 gtgcgactgg agaatggatc gttggtcagt agttcccgat ggttattatc ccgggaggca
    33601 tcgctaagca gtgtcgccag tttgtcgctc agataagggc gcaaggcggt gatgtcgttg
    33661 ctgcggtgca aaatgcggta gtcataaaat tgctgggcca cgttatccgg gcctccttca
    33721 acgcaaggac cactgcgtgt gccgttatct ttataagctg gagtgactgt ggtgcaggca
    33781 ctgaggagca gtgcgcaggg gataagcatt gtcaatttgc tgtagcgcat aatgatttcc
    33841 ttataagcga tcgctctgaa agcgttctac gataataatg atatcctttc aataatagcg
    33901 tatcagtctg ataatgcttt agagatcgaa ggcttaacaa acaaggagat cgatcatgca
    33961 attttctaca accccaactc tggaaggcca gaccatcgtt gaatattgcg gtgtggtgac
    34021 cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta tccgcgatat
    34081 cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg agatcgcctt
    34141 tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtcgtcg gtattgatat
    34201 cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca gcggtacggc
    34261 ggtgaaaacg cgtcgatgag aagagtcttc tggctgatcg ctgccgctct gttactggca
    34321 gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga tacccgacgc
    34381 caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac cgcagatgat
    34441 tttgatagct cgctggcgac actgaccgat aagcaggtca gctcgcatta tctggtccct
    34501 gcggtaccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc ggaacaagaa
    34561 ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa cgacacctct
    34621 attggcattg agctggaaaa ccgtggctgg caaaaatcgg ccggagtgaa atattttgcc
    34681 ccgtttgaac cggcacagat tcaggcgctt attccactgg cgaaagatat tattgcccgt
    34741 tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca gcgcaaagac
    34801 gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg tgcctggccg
    34861 gatgcgcagc gggttaactt ttaccttgcc gggcgcgcgc cgcacacacc ggtagatacc
    34921 gcgtcattgc tggagctttt ggcgcgctac ggttatgacg ttaaacctga tatgacaccg
    34981 cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac gttatataac
    35041 ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa atacgggcag
    35101 gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc gataccttca
    35161 tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat atccagcgta
    35221 aagtcaaaat tcaacttgga gacgccgtag tgggtcagcg gcggctcttt tgctgacttg
    35281 cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaggg gacggaacga
    35341 atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg cagtgtgcga
    35401 tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca ggcttcctgg
    35461 cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac cagcgcactg
    35521 ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag acggggaata
    35581 aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa gcgcgtttgt
    35641 ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct gcgggcaaac
    35701 tcgttggcga agcggtgagg gcgaaaatct tctttaatat cgcgatggtg gtgataatcg
    35761 aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc gacagcccat
    35821 tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg tgtcccccag
    35881 ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat cactttagct
    35941 tgtgatgaaa ccagctcggt cagatccgcc ggaacaaact ctgcgcccat tttttccagc
    36001 aatttgccca ttgcctcgtt gcgaccggtc gctcgcacgc tgatgccttt ctggcataaa
    36061 aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac cttcatatca
    36121 atccactgtt gttgagaaaa taacgtgcgc attcttccgt gatttccccc atgatgcaat
    36181 gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt tctgccagtc
    36241 tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc atcaataacc
    36301 agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg atagtacgat
    36361 agtcgccttt atcttccagg ctaaaacaca gtcgtcccag ccccggcgct ttcatgccaa
    36421 ataacaacgt aagttgtttt tccggttcaa cgacaatcac tttccagcta tctaccgcat
    36481 cgccagtctg taaatattcg cgctccgggc ggcctttcgc cagcttatga ccgatcgcgc
    36541 ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc tctttaccgc
    36601 cgatttggtt cactacctgc cataaagcag caaggctggc ggacgtttta acggtaaacc
    36661 ccgcctgttt ggcaaaataa ccgtactccg gtcgccagcg ggcaaaggcc tgagcgtcgt
    36721 agccccagtc gctggagttg accagttttt cctcctcttt caacgtgcta cgtaccgcgt
    36781 catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca tccgccagca
    36841 gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg gaagtaatca
    36901 cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc aaccagcggc
    36961 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
    37021 cggcggcttc gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc accagatagt
    37081 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
    37141 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa
    37201 cgataattcc ggcccgaagt tcggtcacag gtacattcgc ttcacgaaga atgtccgccg
    37261 tagcctgacg agcacgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga
    37321 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct
    37381 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg
    37441 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
    37501 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga
    37561 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
    37621 cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag
    37681 tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca
    37741 atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca
    37801 ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
    37861 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
    37921 acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg
    37981 ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt
    38041 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
    38101 tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
    38161 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca
    38221 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
    38281 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
    38341 gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac
    38401 agatagttat gcgcggtctg accgacaata tactcttcgc cacgttcgca gtgactgagc
    38461 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
    38521 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
    38581 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca
    38641 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
    38701 agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc
    38761 aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaac
    38821 tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc
    38881 agggcttcat caacttcaga cgctttttct acacggatac ccgtaatgcc gcacgcttcg
    38941 gcaatgcggg caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata gccaccagct
    39001 ttcatctcca tcgccacaaa gcccagcacg ctgttgttaa agacgacaat tttcactggc
    39061 agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
    39121 cacatggcga ccacctgacg ttctggctct gtcgcctgcg cacccagcgc ctgcggcatg
    39181 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttt
    39241 agataacgtg ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat agcgtcatcg
    39301 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
    39361 ttagctaaat cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc tttatccaga
    39421 aacttgcgat cggctttttc ttccaccaat ggaagcaatg cacgcagagt cgacttgata
    39481 tcgccgacca gtgccatatc caccttgctg tgagcgccga tgctggctgg gttgatatca
    39541 atctgaatga ttttggcatc ggtcgggtag aaggcgcggt agggaaattg cgtgccgagt
    39601 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaagcc gattaacccg
    39661 gtcattccaa catcatacgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga
    39721 acaataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
    39781 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
    39841 aactcttctt cttccggcgt cacgactggt tgtggcgcat gataccagtg cgtggttgcc
    39901 ccttctggcg caggttttaa cgccacgtcg cctggtaaca cgacaaccga aacgccacgg
    39961 ttaagcaccg ctttgcgcat ggcaatcgcc agtacttgtg ggatctgctc cgggctggaa
    40021 accaactcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
    40081 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga
    40141 ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccggggcc gcacgatccg
    40201 gcgcagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa ggccgccact
    40261 tcttcgtggc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcactaaga
    40321 ccgttcagag agtcgccagt gactccccag atgcgtttca cccctgccga ttcgagtgtt
    40381 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
    40441 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
    40501 ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa
    40561 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
    40621 agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg
    40681 caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt
    40741 agtgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
    40801 ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
    40861 ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
    40921 acggtacgtg aagctaagtc aggtacacct gccagcagtt cgcgagtgag acgaccagcg
    40981 ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca
    41041 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt
    41101 ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc
    41161 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
    41221 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg
    41281 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
    41341 gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg
    41401 cgatatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
    41461 gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
    41521 ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
    41581 caaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
    41641 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgacg atcgctttct
    41701 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt gacaccgcag cccagtttct
    41761 ctgccagagt gacagccaga ataatcgctg agtaagcatc gttacattga cctgcatcta
    41821 ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
    41881 aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt
    41941 ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
    42001 ggctcaccag atcaatcagc gtatcagcag cgccaagcag cgtctggcga ccaaaaccca
    42061 cggtgatcag gtgcggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga
    42121 tgaccgcaga aaaatcttcg ccatccagat gacgcacacc cggccagcca acgatgcttc
    42181 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
    42241 tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag ccactgccgt
    42301 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
    42361 cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
    42421 ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct
    42481 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
    42541 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
    42601 aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac
    42661 cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca
    42721 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
    42781 ctgcctgacg ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt
    42841 tatcgacgcg cgcgttggca tctacagcca ggcattgcgc tttcagcgcc tcgcgcaggg
    42901 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
    42961 ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac
    43021 gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
    43081 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg
    43141 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
    43201 ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca
    43261 actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta
    43321 ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
    43381 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
    43441 cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg
    43501 gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat
    43561 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
    43621 ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc
    43681 aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
    43741 ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat
    43801 taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat
    43861 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
    43921 cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat
    43981 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
    44041 cgccaacagg ttactggcga ggttatcgag aaacgccaga cttataccca taaaaaagag
    44101 aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg
    44161 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
    44221 gaaaacccag tccttgcaaa gatgaagtcg aaatgcgcga tgacacacta ctgaaagcgg
    44281 aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca tcagaggggg atttttaatc
    44341 ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat aaa
//
LOCUS       DAZGXZ010000022        43858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000022 DAZGXZ010000000
VERSION     DAZGXZ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.022"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(162..959)
                     /gene="rfaP"
                     /locus_tag="ACHKJF_001391"
     CDS             complement(162..959)
                     /gene="rfaP"
                     /locus_tag="ACHKJF_001391"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP0948776.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(952..2076)
                     /gene="waaG"
                     /locus_tag="ACHKJF_001392"
     CDS             complement(952..2076)
                     /gene="waaG"
                     /locus_tag="ACHKJF_001392"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0948777.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(2073..3131)
                     /gene="rfaQ"
                     /locus_tag="ACHKJF_001393"
     CDS             complement(2073..3131)
                     /gene="rfaQ"
                     /locus_tag="ACHKJF_001393"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP0948778.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            3544..4821
                     /gene="waaA"
                     /locus_tag="ACHKJF_001394"
     CDS             3544..4821
                     /gene="waaA"
                     /locus_tag="ACHKJF_001394"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP0948779.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4829..5308
                     /gene="coaD"
                     /locus_tag="ACHKJF_001395"
     CDS             4829..5308
                     /gene="coaD"
                     /locus_tag="ACHKJF_001395"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP0948780.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(5347..6156)
                     /gene="mutM"
                     /locus_tag="ACHKJF_001396"
     CDS             complement(5347..6156)
                     /gene="mutM"
                     /locus_tag="ACHKJF_001396"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP0948781.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVELVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPCLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(6254..6421)
                     /gene="rpmG"
                     /locus_tag="ACHKJF_001397"
     CDS             complement(6254..6421)
                     /gene="rpmG"
                     /locus_tag="ACHKJF_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP0948782.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(6442..6678)
                     /gene="rpmB"
                     /locus_tag="ACHKJF_001398"
     CDS             complement(6442..6678)
                     /gene="rpmB"
                     /locus_tag="ACHKJF_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP0948783.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(6895..7563)
                     /gene="radC"
                     /locus_tag="ACHKJF_001399"
     CDS             complement(6895..7563)
                     /gene="radC"
                     /locus_tag="ACHKJF_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0948784.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            7735..8955
                     /gene="coaBC"
                     /locus_tag="ACHKJF_001400"
     CDS             7735..8955
                     /gene="coaBC"
                     /locus_tag="ACHKJF_001400"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP0948785.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8933..9391
                     /gene="dut"
                     /locus_tag="ACHKJF_001401"
     CDS             8933..9391
                     /gene="dut"
                     /locus_tag="ACHKJF_001401"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP0948786.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            9498..10094
                     /gene="slmA"
                     /locus_tag="ACHKJF_001402"
     CDS             9498..10094
                     /gene="slmA"
                     /locus_tag="ACHKJF_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP0948787.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(10131..10772)
                     /gene="pyrE"
                     /locus_tag="ACHKJF_001403"
     CDS             complement(10131..10772)
                     /gene="pyrE"
                     /locus_tag="ACHKJF_001403"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP0948788.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLILLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(10837..11553)
                     /gene="rph"
                     /locus_tag="ACHKJF_001404"
     CDS             complement(10837..11553)
                     /gene="rph"
                     /locus_tag="ACHKJF_001404"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP0948789.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            11680..12543
                     /gene="yicC"
                     /locus_tag="ACHKJF_001405"
     CDS             11680..12543
                     /gene="yicC"
                     /locus_tag="ACHKJF_001405"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP0948790.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            12731..14002
                     /locus_tag="ACHKJF_001406"
     CDS             12731..14002
                     /locus_tag="ACHKJF_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0948791.1"
                     /translation="MTKAKLTDIQIKAWIKAGERFDARADGNGLYLCFPEKYNAPFWR
                     YRYRMAGKQRAMHIGSYSDLSLSKARATVKELAARVALGHDVAGEKQARKAAALARIE
                     EEKNALRVSELAAEYYERQILPRWKHPDILRRRIDKDINPQIGSLKVEDVKPRHIDAM
                     LQRIIDRGAPTVANDVLRWTKRIFDYAIKRHALETNPAAAFELSDAGGAEKSRDRWLT
                     RDELIHFFQAMRTAKGFSRENELTMKLILCLCCRKMELCAAQWDEFDLDSAVWRLPAE
                     RAKNGDAIDIPLPPPAVAWLRELHSISGGSRWVLPARKMQQRMIPHIQESTLPVALAK
                     VRREMPGVPGFTVHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFNER
                     RHALELWADLLVALEQGDEYNVTPITKAIAK"
     gene            14098..15051
                     /locus_tag="ACHKJF_001407"
     CDS             14098..15051
                     /locus_tag="ACHKJF_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948792.1"
                     /translation="MYENTYPIDTAASILGCTQKKILTEWAKGTIKIALDFGPESSIN
                     RQSYTDYDKWGYPAKPWNIASISAPATNDYFPPELLQPEEGEKLRLFPNDNEPESILI
                     WRNVYHQYSQSGFYFDSNHSDAQVHDGKLMVVSDVLLYGLWTVPYAEFSRVHAQDTFT
                     LKPYTDTPSLSKVTATFTPRGGSRRINAPEKKNLRITDSEMMVMQKILAQRPGYQRTE
                     QPTDDRNQEKHDIHGGVERFALEREKVLAAALYVAYHFPKDVGKTLKSHAEAIEKHGY
                     LFWDGNPTPTPDAGRIAKILSDARRLPNEWKILGGNAKAKR"
     gene            15221..15427
                     /locus_tag="ACHKJF_001408"
     CDS             15221..15427
                     /locus_tag="ACHKJF_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0948793.1"
                     /translation="MDTLPKSGYMRPAQVINHFQISESTLWNWVKKGKFPSPDKLGDG
                     VSPFDVSAIRQWLADRKGTTSTRN"
     gene            15449..16054
                     /locus_tag="ACHKJF_001409"
     CDS             15449..16054
                     /locus_tag="ACHKJF_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0948794.1"
                     /translation="MKMPERPATGPGRSHAQNNHHTPHRMGGNRKYCLPAIAGGRYIS
                     PAGVNPPAGRGNPFNEQKATQTRPASFFVSCHRATMNSGKARTVSMVALVGQPKGWPV
                     SFCAGISTPASVTAPYERGNSGGDSLNLQKEAARMATTPTPLHPEFTFHFLAVRRSDL
                     NDRPHRESVNAPDETTARRMLARDFVLLFAGRVPAQGVSHA"
     gene            16047..16511
                     /locus_tag="ACHKJF_001410"
     CDS             16047..16511
                     /locus_tag="ACHKJF_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0948795.1"
                     /translation="MLKNMLIASSFGLYDAGVTHMVTGFSSLNNIADSRLHSDMRFFC
                     VRKPTHTRIMAGRDGGAFALAGFYVASLLTPLRLATPFSSVVARLLTSNIGAFHMAVF
                     ARPYFVWRFMQCQEKQTSLFTVTAATEREARAQLPHAHMIFVARIRQGARHA"
     gene            16504..16800
                     /locus_tag="ACHKJF_001411"
     CDS             16504..16800
                     /locus_tag="ACHKJF_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948796.1"
                     /translation="MRDCLRESMKAAMSSMPDEESHWILRVDADWHRVNLLAGIAFVG
                     KALEGSQLRENPITYSRDEICQLAGFLQTAPALIGCMAELMECYDQQAGEVSHV"
     gene            16793..17107
                     /locus_tag="ACHKJF_001412"
     CDS             16793..17107
                     /locus_tag="ACHKJF_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948797.1"
                     /translation="MCDMYNTRIPELLVAAIKNADAQEARALFDDADYCARKLLEGLI
                     STGRLLSGMGDNLDPSMGELRSLGDSIAVTAELVAGFSEVVEAYNWRCRTGEIREGGQ
                     HA"
     gene            17100..18143
                     /locus_tag="ACHKJF_001413"
     CDS             17100..18143
                     /locus_tag="ACHKJF_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP0948798.1"
                     /translation="MRDIATINAIRSVAAVAAQHAEILVPEWLPEGKRQATEWVCRNP
                     TRADRHAGSFFVSLVDGCWHDFATGDSGGDLVALGAYLWNVRQTDAARIVADRLGLCL
                     PALGKPDVMTREQREAQRVRVQAAEQEAGRLRQREQQQRHQRQKLTACRAFELLGRAE
                     VATPSHPYLMKKRLQPGNLYQSGNDLLVPLYNVCGEVVNVQIISPDGRKMFLRDGQVQ
                     GAFHVMGSFDLMAPEAPEHDVYVCEGWATGAALLQYWNVMAVVCAMNAGNLKHVAMAL
                     RERYGSRISLVIAGDDDRASKGNPGSRAANEAALLAGCYVTFPEWPPGASPDLSDFND
                     LMLWEMEHDPDNH"
     gene            18124..19962
                     /locus_tag="ACHKJF_001414"
     CDS             18124..19962
                     /locus_tag="ACHKJF_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HFP0948799.1"
                     /translation="MTRTTTKTPVTSGKRKPPRKKHPKVSPARPGYATYSQHVQVDGG
                     QILKPGLYWHGVRDSTNTDTFICSPLEVIAMTSDSKDENYGRLLRFLTPSGQWREWAA
                     PMEMLAGDGGELRQELLRMGVTIPYKQRQALTEYIMESAPKRRALAATATGWHSPTLF
                     VMPGGVIGDGDVVFQSTEAGSQEYATAGTPEGWRDNVAALCRGNPVAMLAVCASLAGP
                     LLWLLDVDSGGFHFMGDSSSGKSVGALVACSVWGEPRNFKRNWNATSTGLEGLATMRN
                     DTALILDEIGEAPARDIGGLIYQLGNGTGRQRGKVSGMARPVNTWRTMIISTGEMTVG
                     KHMESGGQRIKAGQEMRLLDIPAQRLYGAFDCLHGMALPHNPDNDTERRGAGRKFAET
                     LRGNAATHYGHAGPEFVRWLISRNESDTTSTDSNGQTLADMYAILREQFPVSTGQEAR
                     AAARFTLCALAGEMAITAGILPWKQGEALEAVKAMFAAWSDYRGRGQSEDVKILRAVA
                     GFIARHASRFEGGNTYSEPVSNRAGWWRPGSNGDRVYWFLPEALCEAASGYDLKRIAA
                     SLDDAGAIVDRDTDGKRRYTKRVSVNGEKHRVYAIDQNALLFAIDE"
     gene            20662..20952
                     /locus_tag="ACHKJF_001415"
     CDS             20662..20952
                     /locus_tag="ACHKJF_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948800.1"
                     /translation="MHKLFSLPDALCEFIRALPAPDLQEAHQSETWPEDNTTSNGQTL
                     ADMCAPSAAWLAARDAYISHVMTCPHCATSGVKKPRHCAEGERLRQNYDHTR"
     gene            21378..21623
                     /locus_tag="ACHKJF_001416"
                     /pseudo
     CDS             21378..21623
                     /locus_tag="ACHKJF_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            21635..21931
                     /locus_tag="ACHKJF_001417"
     CDS             21635..21931
                     /locus_tag="ACHKJF_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0948801.1"
                     /translation="MVHDRIAEELESKGFYRRAAARWAEVMMLVETDRERERVAMRRA
                     ECYGKAQRPPEPPEENYADLRKAVNRTYAEMGIGGRCKKSGVATRNKHRAGTVG"
     gene            22021..22428
                     /locus_tag="ACHKJF_001418"
     CDS             22021..22428
                     /locus_tag="ACHKJF_001418"
                     /inference="COORDINATES: protein motif:HMM:NF020345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP0948802.1"
                     /translation="MNEQTHHVPKDIERLKFMNDIDGGDKPGDAWCDSLADIVSHSVA
                     ELTRNGMEEADAFRIAKRVVAAIAFYAGGRSLYLPTGRSLFAALREYEIYYRWDNGEK
                     LQALRKEYAISETHIYDIIRRLRKKHANKITQD"
     gene            22811..22892
                     /locus_tag="ACHKJF_001419"
     tRNA            22811..22892
                     /locus_tag="ACHKJF_001419"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22849..22851,aa:Tyr,seq:gta)
     gene            23304..23906
                     /gene="dinD"
                     /locus_tag="ACHKJF_001420"
     CDS             23304..23906
                     /gene="dinD"
                     /locus_tag="ACHKJF_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP0948803.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQANTTHFD
                     VGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            24197..24814
                     /gene="yicG"
                     /locus_tag="ACHKJF_001421"
     CDS             24197..24814
                     /gene="yicG"
                     /locus_tag="ACHKJF_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP0948804.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(24811..26493)
                     /gene="ligB"
                     /locus_tag="ACHKJF_001422"
     CDS             complement(24811..26493)
                     /gene="ligB"
                     /locus_tag="ACHKJF_001422"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP0948805.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            26751..27374
                     /gene="gmk"
                     /locus_tag="ACHKJF_001423"
     CDS             26751..27374
                     /gene="gmk"
                     /locus_tag="ACHKJF_001423"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP0948806.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            27429..27704
                     /gene="rpoZ"
                     /locus_tag="ACHKJF_001424"
     CDS             27429..27704
                     /gene="rpoZ"
                     /locus_tag="ACHKJF_001424"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP0948807.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            27723..29831
                     /gene="spoT"
                     /locus_tag="ACHKJF_001425"
     CDS             27723..29831
                     /gene="spoT"
                     /locus_tag="ACHKJF_001425"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP0948808.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            29838..30527
                     /gene="trmH"
                     /locus_tag="ACHKJF_001426"
     CDS             29838..30527
                     /gene="trmH"
                     /locus_tag="ACHKJF_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP0948809.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            30533..32614
                     /gene="recG"
                     /locus_tag="ACHKJF_001427"
     CDS             30533..32614
                     /gene="recG"
                     /locus_tag="ACHKJF_001427"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP0948810.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(32599..33465)
                     /locus_tag="ACHKJF_001428"
     CDS             complement(32599..33465)
                     /locus_tag="ACHKJF_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948811.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(33468..34673)
                     /gene="gltS"
                     /locus_tag="ACHKJF_001429"
     CDS             complement(33468..34673)
                     /gene="gltS"
                     /locus_tag="ACHKJF_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP0948812.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            34953..36344
                     /gene="xanP"
                     /locus_tag="ACHKJF_001430"
     CDS             34953..36344
                     /gene="xanP"
                     /locus_tag="ACHKJF_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP0948813.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            36465..38174
                     /gene="yicH"
                     /locus_tag="ACHKJF_001431"
     CDS             36465..38174
                     /gene="yicH"
                     /locus_tag="ACHKJF_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0948814.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(38227..40545)
                     /gene="yicI"
                     /locus_tag="ACHKJF_001432"
     CDS             complement(38227..40545)
                     /gene="yicI"
                     /locus_tag="ACHKJF_001432"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP0948815.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(40555..41937)
                     /gene="yicJ"
                     /locus_tag="ACHKJF_001433"
     CDS             complement(40555..41937)
                     /gene="yicJ"
                     /locus_tag="ACHKJF_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP0948816.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            42230..42324
                     /locus_tag="ACHKJF_001434"
     tRNA            42230..42324
                     /locus_tag="ACHKJF_001434"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42264..42266,aa:Sec,seq:tca)
     gene            42624..43808
                     /locus_tag="ACHKJF_001435"
     CDS             42624..43808
                     /locus_tag="ACHKJF_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0948817.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
BASE COUNT        11135 a        11681 c        11037 g        10005 t
ORIGIN      
        1 ccaaaaagaa aatttctgtc aataccaaat gctatatcaa gagtattttt agatcttata
       61 ttttgataat gaaaattata ctcaaaaagg atgatgtcac tatcattgag ttgagacata
      121 tttattctgt atccaaaatt ttggcaaaaa taagttacgg gttacaacga ttttcgaatc
      181 gttctttccc tgatttttgt ggcttttgct tctgcttgcg acagcagtcc ttgttcctgc
      241 ttgagaatgt ctttaagcgg ggcggcaaaa tacactttca taaaccgcca gatatcccgc
      301 tgagtcaggc cgatattcat cgaagaaaaa taaagcccaa taagatcttt atcccgccaa
      361 cgacgtggaa cgcgcgtgcg aagctgcgcc cggtgcaggt caattaccga aatttttaac
      421 tcctcttcct taccggaaaa aggcaagtgc agcaggaaat gacagatata acagtcacgg
      481 tggttaatgc ccgcagcatg catatcgcgc accatcgtcg cgacacgctt aataagcata
      541 cgctttacgc gaacatctgg agggttagtc gcccagtcag cactgtaatc ttcaagactt
      601 atggttggtg tcagatcttc ggtaataata aatgaagttc tggtcagcgg attcatgcct
      661 ttttcgccaa atgccacccc atacatagta tcaacgccga catcccgcag tcgatgaatc
      721 gcattccatt cgcggtcagc gcctaatact ggcatccgca atgagagtaa atttttgatt
      781 atctctttca gggtcgtgcc gcgatgccat ttgagaaaat agcttttgcc cgccatttca
      841 aagcgcagag tacggcgagt ttccagttca cgaaatacct caccctgcaa ggttttaact
      901 tcctcaaaag gatctttgcc gcgccataac gtggcaaacg gctctttaag ttcaaccatc
      961 cagaccaccc gttatgatat ccgccgcttt ctctggcaga ctgtataaat cttgtgtatc
     1021 agcataatgt cgcgcatttt ccgcccaggc ctggcgcaat gaagattgcg ttaacgcttt
     1081 gcgtaaaatc tcattcaatg tttcctggcg gaatggctca gcaatagcct cgccgcaatt
     1141 agcgtcgaca atataatgcg catagccaca aacggctgtt gttagtaccg gtaatcctgc
     1201 agtaatcgct tccagcagca caattcccgc cgcttcctgg taggcaggat gcagtaataa
     1261 atccgccgcc gccattaatt ccgagacatc attgcgcccc gagaaaaagt gaacattact
     1321 gcgcacgccg cgtttttctg ccagcgcctc aaattttcgc ggtttatcct gcccaacaac
     1381 atatagcaat gtgttgtggc gcagcgaatc cggtaacgaa gcaagtgctt caatggaacg
     1441 atcgacacct ttacgcgtga agtctgaacc gacctgcaac aataaatatt gttgttcggt
     1501 tattccattc ttcttacgga agatttcacg gctattggct ggctgctggc tatatttacg
     1561 atcaggataa atccccggtg gcagaatatg aaaacgctcc gcttcagtct gataatgttt
     1621 ctggaaatcg gcgatttgct tatctgtcag catcagcaac tgtgtcggct taccctgttc
     1681 gaaggttgcc cgctcaaagg cggcataatg gcgataacgt gacgtcaggc gatagaaaaa
     1741 gcctttttcc tgcgctactt tctcggcata acaaacatca gcggcataat aaacgtccag
     1801 ccccggcatt ttgttgaatc caacgacttt atcgacggga tgttcgcgta aatgtttttg
     1861 cacccaggca aaatactccg cattgcgccc gtgattggta tgcgatttaa ccggcacttt
     1921 gatcagttca aatacatcag ggcattcgcc ttcccacgac tgggtataaa cccgaacatg
     1981 atgacctcgg gcggcgactg tctgagcaat acgcataaaa tcacgctgca aaccgccaaa
     2041 gggaaaatat ttatataaac aaaaagcaac gatcataacg acgtacctgt cgtggaggag
     2101 ggcagtaatt tatcgacagc agcaatgaca tctgccgccg gaataaccga aagatacatc
     2161 tcatttcggt cacgctgatc gcgcgttggc atttcccggt aatctcccgc ccagaattga
     2221 atcatgttat ttgaccaggg acgccagaaa atatgatctg tcgcaccaaa cagcgatatc
     2281 agcggcgtat taactgcagc ggcaatatgc gccggtgcgg aatcaacgcc aataaacagc
     2341 tgcgcatgat cgattaacgc accaagttcc gggaaggtca cctttccagc cagcgccgtt
     2401 actggtggcg tctggcatcc ctgcgcaatt tcattgacgc aggccagatc gtctttatct
     2461 gggccggacg tcagaacgac ttcataacca cgagcatgta aggcatcaat cacagcggaa
     2521 aacttggcgt tgtcccagca tttgaagatt tgccgcgccg taggttggat aaccacataa
     2581 ttttgtccaa caccagcgtg atcaagttcg cgacgcatac gtttccagct tgcaggcggg
     2641 taactcatgg ttgtctgctt caccaacgaa tcaagtccca atggtgtcag cacggataag
     2701 ttactttcca ccacatttcc accctgcaac ggcaccaaat gggtgaaact tttacgccaa
     2761 aaagcagact gccgatgatg ataatcctgg gaaattttca cacgagcatt taataagcga
     2821 accagtatag caaccatcca ttgatcggta agattgacga taaggtcata cttattggca
     2881 cgtaatacct tgatgagatg aaaaaagttg gcaatttttt ctgaggcttt tgctttttta
     2941 ttttttatgc cgtagagcgc gttaatctct ggattttcag acaggatcgg gatggtgtcc
     3001 tgataaagca gcacatcgat ttttgcgtca gggtaatttt ttttcagcga actaatgacg
     3061 ggagtagtta ataacatatc cccatgaaaa cgcatcttaa tgagcaaaat tcttcgaaat
     3121 ggcttatcca caagtaactc tttttgttga gattatccga ttaaggtgaa gcagaaaaag
     3181 cacgctaccg ccccaggctc aacagctacc tgaatactga taccgcactg atattgacgc
     3241 gctagtgtat cacttctttc gcggcaatcc gacccgaatg atttttaagc acattgttga
     3301 ttttcaataa agaaatcaat caaataaaat cattagcaac catattcttt cactgttgct
     3361 ctgcgattcc ttcctgcacg tacagcagaa atcaatgaat cgcataaaaa tacaaaaaaa
     3421 gtatagatgt tcagcaagaa ttggcgggta aattgctatt taagtcaaaa acgggaaaag
     3481 taatggtaaa gccacagcta aatacataga atccccagca catccataag tcagctattt
     3541 actatgctcg aattgcttta caccgccctt ctctacctta ttcagccgct gatctggata
     3601 cggctctggg tgcgcggacg taaggctccg gcctatcgaa aacgctgggg tgaacgttac
     3661 ggtttttacc gccatccgct aaaaccaggc ggcattatgc tgcactccgt ctccgtcggt
     3721 gaaactctgg cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc tgatttaccg
     3781 attaccgtta caaccatgac gccaaccggt tcggagcgcg tacaatcggc tttcgggaag
     3841 gatgttcagc acgtttatct gccgtatgac ctgcccgatg cactcaaccg tttcctgaat
     3901 aaagtcgacc ctaaactggt gttgattatg gaaaccgaac tatggcctaa cctgattgcg
     3961 gcgctacata aacgtaaaat tccgctggtg atcgctaacg cgcgactctc tgcccgctcg
     4021 gccgcaggtt atgccaaact gggtaaattc gtccgccgct tgctgcgtcg tattacgctg
     4081 attgctgcgc aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc aaaaaataac
     4141 caggtaaccg ttaccggtag cctgaaattc gatatttctg taacgccgca gttggctgct
     4201 aaagccgtga cgctacgccg ccaatgggca ccacaccgcc cggtatggat tgccaccagc
     4261 actcacgaag gtgaagagag cgtggtcatt gccgcacatc aggcattgtt acagcaattc
     4321 ccgaatttat tgctcatcct ggtaccccgt catccagaac gcttcccgga tgcgattaac
     4381 cttgtccgcc aggctggact aagctatatc acacgctctt caggggaagt cccctccacc
     4441 agcacgcagg ttgtagttgg cgatacgatg ggcgagttga tgttactgta cggcattgcc
     4501 gatctcgcct ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc gctggaagct
     4561 gccgcacacg ctattccggt attgatgggg ccgcatactt ttaactttaa agacatttgc
     4621 gcgcggctgg agcaggcaag cgggctgatt accgttaccg atgccactac gcttgcaaaa
     4681 gaggtgtcct ctttactcac cgacgccgat taccgtagtt tctatggccg tcatgccgtt
     4741 gaagtactgt atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct ggaaccttac
     4801 ctgccaccga aaacgcattg aggttgttat gcaaaaacgg gcgatttatc cgggtacttt
     4861 cgatcccatt accaatggtc atatcgatat cgtgacgcgc gccacgcaaa tgttcgatca
     4921 cgttattctg gcgattgccg ccagccccag taaaaaaccg atgtttaccc tggaagagcg
     4981 tgtggcgctg gcacagcagg caaccgcgca tctggggaac gtggaagtgg tcgggtttag
     5041 tgatttgatg gcgaacttcg cccgtaatca acacgctacg gtgctgattc gtggcctgcg
     5101 tgcggtggcg gattttgaat atgaaatgca gctggcgcat atgaatcgcc acttaatgcc
     5161 ggaactggaa agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct cttcatcgtt
     5221 ggtgaaagag gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg agaatgtcca
     5281 tcaggcgctg atggcgaagt tagcgtagcg tttatgccgg atggtatgcc atccggcgcg
     5341 catgaattac ttctggcact gccgacaata aaacgttgcc cgctgcgcat gtttagtcgc
     5401 cacaatcggc gtaccgcaca cccgacacgg ctcgcctttt cgcccgtaaa cctgcaattc
     5461 ctgagcgaaa tagcccggtt taccatcact ttgcagaaaa tctttcagcg ttgtaccacc
     5521 ctgctcaatc gaacgcagca acaccgcttt aatcacccga gctaacaatt cacactctgc
     5581 cagcgacagt gatgacgcca gccgatccgg atggatcccc gccgcaaaca gtgattcgct
     5641 ggcatagata ttccctaccc ctaccaccag cttgttatcc atcagacacg gtttaatcgc
     5701 cgtttttttc ttcgcgcact tctgatgcag atactcacca ttgaaatcgt cgctaagcgg
     5761 ctccggtcca agatgggtca gcacattatg cccttccagc tctttggtcc acagccaggc
     5821 accaaagcgg cgcggatcgg tgtagcgcag cactttgccg ttgctcatca ccaattccac
     5881 atggtcatgc ttttcagggg gaagttcttc tggaaggatg cgcaggctgc cagacatccc
     5941 taaatgaatg ataatccagc cctcaggcag ctccagcagc agatatttag cccgccgctg
     6001 cacgctaagc actggttggt cgcttaaacg gtagatctct tctgaaaccg gccagcgcaa
     6061 gcgtccgttg cgcaccactg catgaagaat ggttgcacca acgagatgcg gttctatgcc
     6121 gcggcggctg gtttcaactt cgggtaattc aggcatagca tctccaggaa tgaacagatg
     6181 cagtcaatat gggggcaagc agataacaaa aaaccccgcc ggagcgaggt tttttgttac
     6241 atcaaagcga gaattatttg attttcgctt ctttgtagat cacgtgctgg cgaacaactg
     6301 gatcgaattt tttcagttcc agtttttccg gcttagtacg tttgttcttc gtagtggtat
     6361 agaagtgacc agtaccagca gaagaaacca gcttgatttt ctcacgaata cctttagcca
     6421 tgatttattt cctctaagta cttagtactt ttcgccacgg gcacgcagtt cagccagaac
     6481 tgtatcgatg ccttttttat cgattacacg cataccttta gcagatacgc gcagggtgac
     6541 aaaacgcttc tcgctctcaa cccagaaacg gtgagagtgc aggttcggca ggaaacggcg
     6601 tttagtcgcg ttcagtgcgt gggaacggtt gttaccggtc accggacgct tgccagtaac
     6661 ttggcagact cgggacatgt ctattctcca aaaatcaaat tagctcgagc ttcgtatggg
     6721 gtattggcgc ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg aactctcgat
     6781 tgccaggccc aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac gctgactcag
     6841 cgatgtgctc aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag cgggttaaat
     6901 ccagccgcgt tcggcaaaag aaacatactc tccacgccca atcacgatat ggtcgagcac
     6961 gcgtaaatcc atgaactgac aactctttat tatccgttca gtaatgagtt tatccgcttt
     7021 actgggttca gcacaacccg aagggtgatt atgtgcaagg atcagcgccg aggcgtttat
     7081 ttttatcgct tcgcggataa tttcccgagg atggacttca acatggttta gcgtgccgga
     7141 aaaaagacgg ctatgcgtta taacccggtg ttgggagtcg agaaagatca ccataaagat
     7201 ctcccgctcc tcacccgtga gctggctttg taaaaattcc ctcgtcatct ccgggctgag
     7261 taaagggctt tcttcacgca tccgcacgtt gtagtaacgc cgcgccagtt cagcaatccc
     7321 ctttaactgg gcaaatttcg ccacgccaat tccatgaacg ccactaaatt gttcatattc
     7381 agaggttaac aagccataaa gagagccgaa attctccagc atctcttttg ccagggttaa
     7441 tacatcttta ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga catccgttaa
     7501 ggcgctaata ccaaacttca gcattttttc gcgcggcatc aacagctgtg aattgttttt
     7561 caccttcacc tcctttgtgg tgctcgcatc ctgtcatagc cgtttcgtta aatcgacggc
     7621 cagttttcaa tcctggaaag cgcctcgcaa agtgaatctc aggtgaaggc acgacaacaa
     7681 aagtattgtg ataaaatcgc caacttctgg tgtcacccta caggaaaaat catcatgagc
     7741 ctggccggta aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta taaaacccct
     7801 gaactggtgc gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat gaccgaagcg
     7861 gcaaaagcct ttatcacccc acttagcttg caggcggttt ctggttatcc cgtttccgac
     7921 agtctgctgg acccggcagc cgaagccgct atgggccata ttgagctggg taaatgggct
     7981 gatttagtga ttctcgcccc tgccacggca gatttgattg cccgtattgc tgccggaatg
     8041 gcgaatgacc tggtatcgac gatttgtctg gctacacctg cgcctgtagc cgtgctcccc
     8101 gccatgaacc agcagatgta ccgtgccgct gccacgcagc ataatttaga ggtgcttgct
     8161 tcccgtggta tgctcatctg ggggccagac agtggcagtc aggcttgtgg tgatatcggt
     8221 cctgggcgaa tgctcgatcc gttaaccatt gtggatatgg cggtggcgca tttttcgccc
     8281 gtcaacgacc tgaaacatct gaacattatg attaccgccg gcccgacgcg tgaaccgctc
     8341 gatccggtgc gttatatctc taatcacagc tccggcaaga tgggttttgc tatcgccgcc
     8401 gccgctgccc gtcgtggcgc gaacgtcacg ctggtatcag gtccggtttc actaccgacg
     8461 ccaccgtttg ttaaacgtgt tgatgtgatg actgcgctgg aaatggaagc cgccgtgaat
     8521 gcttctgtac agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga ttatcgcgca
     8581 gctaccgtgg ccccagagaa aatcaaaaag caggccacgc agggtgatga attaacaata
     8641 aaaatggtta aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga ccatcgaccc
     8701 tacgtcgttg gatttgccgc cgaaacaaat aatgtggaag aatacgcccg gcaaaaacgt
     8761 atccgtaaaa accttgatct gatctgcgcg aacgatgttt cccagccaac tcaaggattt
     8821 aacagcgaca acaacgcatt acaccttttc tggcaggacg gagataaagt cttaccgctt
     8881 gagcgcaaag agctccttgg ccaattatta ctcgacgaga tcgtgacccg ttatgatgaa
     8941 aaaaatcgac gttaagattc tggacccgcg cgttgggaag gaatttccgc tcccgactta
     9001 tgccacctct ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg ccgtagaact
     9061 ggctccgggt gacactacgc tggttccgac cgggctggcg attcatattg ccgatccttc
     9121 actggcggca atgatgctgc cgcgctccgg attgggacat aagcacggta tcgtgcttgg
     9181 taacctggta ggattgatcg attccgacta tcagggccag ttgatgattt ccgtgtggaa
     9241 ccgtggtcag gacagcttca ccattcaacc tggcgaacgc atcgcccaga tgatttttgt
     9301 tccggtagta caggctgaat ttaatctggt ggaagatttc gacgccaccg accgcggtga
     9361 aggcggcttt ggtcactctg gtcgtcagta acacatacgc atccgaataa cgtcataaca
     9421 tagccgcaaa catttcgttt gcggtcatag cgtgggtgcc gcctggcaag tgcttatttt
     9481 caggggtatt ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt cgcgaggaaa
     9541 tacttcagtc tctggcgctg atgctggaat ccagcgatgg aagccaacgt atcacgacgg
     9601 caaaactggc cgcctctgtc ggcgtttccg aagcggcact gtatcgccac ttccccagta
     9661 agacccgcat gttcgatagc ctgattgagt ttatcgaaga tagcctgatt actcgcatca
     9721 atctgattct gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc
     9781 ttctcggttt tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctaa
     9841 tgtttgaaca ggatcgcctg caagggcgca tcaaccagct gttcgagcgt attgaagcgc
     9901 agctgcgtca ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac accaccgatg
     9961 aaaccctgct ggcaagccag atcctggcct tctgtgaagg tatgctgtca cgttttgtcc
    10021 gcagcgaatt taaataccgc ccgacggatg attttgacgc ccgctggccg ctaattgcgg
    10081 cacagttgca gtaatatgac gccggatgac ttttcatccg gcgagtttct ttaaacgcca
    10141 aactcttcgc gataggcctt aaccgccgcc agatgttccg ccatttccgg cttctcttcc
    10201 aggtaagcaa tcaggtcttt cagggtgatg atagagatca ctttgcagtt gtaatcacgc
    10261 tcaacttcct gaatcgccga aatctcgccg cgcccccgtt cctgacgatc aagcaaaatc
    10321 aacacgccag caagcgtcgc gccattagcc tgaataatct ccatcgattc gcgaatcgcc
    10381 gttccggcgg tgatcacatc atctaccagc attacgcgtc cttgtaacgc gctaccaacc
    10441 agattgccgc cttcaccgtg gtcttttgcc tctttgcggt taaagcagta cggcaggtca
    10501 aggtcatgat gctccgccag cgccacggcg gtagtggtgg caatcgggat ccctttgtaa
    10561 gcagggccaa acagcagatc gaactcaagg ccggaatcca ccaacgcttc agcgtaaaaa
    10621 cggcctaaca gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa gaaatagggg
    10681 cttttgcgcc cggacttcaa cgtaaactcg ccaaacttta acacctgctt gccaagcgca
    10741 aattcaataa actggcgctg atatggtttc atgccttcgc tcctcatctt acttttctac
    10801 agacaaaaaa aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg
    10861 cgtcgctaca atggattcga ttccccctcg ggccagcgcc aacaaggtga gtaactcttc
    10921 atgggtgaac ggctcgcctt ctgccgtccc ctgcacttca ataatgcgcc cgtcttcggt
    10981 catcactacg ttcatgtcgg tctctgcggc tgagtcttca acgtattcca gatcgcaaat
    11041 tgcttcaccg tttacaatac cgacagaaac cgcggctacc atccctttca ttggattggt
    11101 tttcagcttg ccgttttcca ccagcttctg tagcgcatct gccagcgcca cacaggcacc
    11161 cgtaatcgaa gcggtacgcg tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat
    11221 ggtgaactca cccagcgctt tcaaatctac tgccgcgcga agtgcacggg cgatcagacg
    11281 ctggatttcc attgtgcgtc caccctgctt acctttcgct gcttcacgag cgttacgggt
    11341 gtgggtagaa cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt
    11401 caggaagcgc ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa
    11461 ttcgaccagc accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg
    11521 cacctgatta ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg
    11581 tggctgcgca ttatacggac ttccggcggt tattcctatc ctgacaaggc atcgatggct
    11641 ataatcctcc cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc
    11701 tacgcccggc gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta
    11761 aaccagcgtt atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct
    11821 gtcgttcgcg agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc
    11881 tatgagccag atgttagcgc acaaggtgag ctgatcctca acgaaaaact ggctaaacag
    11941 ctggtaactg ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt
    12001 gatattctac gctggccggg cgtgatggca gcccaggagc aggatctcga cgcgattgcc
    12061 gctgaaattc tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc gcgcgaaacc
    12121 gaaggtcagg cgctgaaagc attgatcgag cagcgtctgg aaggcgtcac cgccgaagtg
    12181 gtcaaagtcc gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg
    12241 aagctggaag atgctcaggt tcagttggaa aacaaccgtc tggagcagga gttggttctg
    12301 ctggcacaac gaattgacgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag
    12361 acctacaaca ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcag
    12421 gaattcaacc gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacaaac
    12481 tccgccatcg agctgaaagt gttgattgag cagatgcgcg agcagattca gaacgtggaa
    12541 taacccccag ccaactccga ggcactccac acaaaaacag caaagcccca ttctatgggg
    12601 cttttttact gtaatgcact ccgatatact ccaaagcaat ccagcaaaaa ccgtgtacat
    12661 aaacgtgtac atactgggtt tatggacaca tttttatgta cacactccca ttcaatattc
    12721 aggtggagtt atgacaaagg cgaagttaac ggatattcag ataaaggcgt ggataaaggc
    12781 cggggaacgc tttgatgccc gcgccgatgg taacggcctg tatttgtgct tccctgaaaa
    12841 atataacgcc ccattctggc gctatcgtta ccggatggcc gggaaacaac gtgctatgca
    12901 catcggctca tattccgacc tgtcactaag taaagcccgc gccaccgtta aagaactggc
    12961 cgcccgtgtt gcgctgggcc atgacgtggc cggagaaaag caggcccgaa aagcggcggc
    13021 actggcacgg attgaggaag aaaaaaacgc ccttcgcgtg tcagaactgg cagccgaata
    13081 ctacgaacgg caaatactac cccgctggaa acatcccgat attctccgcc gccgcataga
    13141 caaagatatc aaccctcaga ttggcagcct gaaagtcgaa gacgttaaac cgcgccatat
    13201 tgacgcaatg ctacagcgca ttattgaccg cggagcgcca acagtcgcca atgatgtttt
    13261 gcgatggact aaacggatat tcgactacgc catcaaacgc cacgcactgg aaacaaaccc
    13321 cgccgctgct tttgagttat cagatgctgg tggggctgaa aaaagccgcg accgatggct
    13381 aacccgtgat gaactcattc atttttttca ggccatgcgc acagctaaag gattcagccg
    13441 tgagaatgaa ctcaccatga aactgatact ttgcctttgc tgccgaaaaa tggagttgtg
    13501 cgccgcacag tgggatgaat ttgatttaga tagtgctgta tggcgactcc ctgccgaacg
    13561 ggctaaaaac ggggatgcca ttgatatccc actaccgcca ccagcggtgg catggttgcg
    13621 cgaattacac agcatttcag gcggtagccg ctgggtgctt ccggcccgca aaatgcaaca
    13681 gcgcatgatt ccacacatac aggaaagcac cctgcccgtt gcgctggcta aggttcgccg
    13741 ggaaatgcct ggtgtgccgg gttttaccgt tcacgatttc cgacgcacag ccagaactca
    13801 ccttgccgcg cttggggtag accctgtagt ggcggaacgc tgcctgaacc accgaattaa
    13861 aggcgttgag ggtatctata accgccatca gtatttcaat gaacgccgcc acgctctgga
    13921 gttatgggcc gacctgctgg tggctctgga acagggggat gaatacaacg taacccccat
    13981 cacaaaagcg attgcaaaat aaaaacatac cggggatagc gcgacggcgc gaaaagcggg
    14041 aaaccttacc gcctgccccg gtttattttt aaggtagcgt caaaggtaac gtaaaccatg
    14101 tatgaaaaca cataccctat tgatacagcc gccagcatac tggggtgtac tcaaaaaaaa
    14161 atactgactg aatgggctaa agggacaata aaaatcgcac ttgatttcgg cccggaaagt
    14221 tccataaacc gccaaagcta cacggattac gacaagtggg gatatccggc caagccgtgg
    14281 aacattgcca gtatttcagc gccagcaaca aacgattatt tcccacctga attactacaa
    14341 ccggaagaag gggagaaact caggcttttc cccaatgaca acgaacctga atcaatattg
    14401 atttggagaa atgtttacca tcaatactca caatccgggt tctactttga cagcaaccat
    14461 agtgacgcgc aggtacatga cgggaaactt atggttgtat cggatgttct cctgtatggc
    14521 ttatggactg taccttacgc cgagttctcg cgcgttcacg cacaggacac attcacatta
    14581 aaaccgtaca ccgatacccc ttcactgtcc aaagttacag caacctttac accacgtggc
    14641 ggctcacgcc ggataaacgc cccggaaaaa aagaatttgc gcattaccga ttcagaaatg
    14701 atggtcatgc aaaaaatact tgcacaaagg cccggctacc agcgcacaga acaaccgaca
    14761 gatgacagga atcaggaaaa gcatgatata cacggcggtg tggagcgatt cgcgctggaa
    14821 cgtgaaaagg ttctcgcggc tgccctgtac gtggcgtatc acttccctaa agatgttggc
    14881 aagacactta aatcacatgc cgaagccata gagaaacacg gttatctgtt ctgggatggc
    14941 aatcccaccc caacacctga cgcgggacga atagcaaaaa tattatctga tgccagacgc
    15001 ttacccaatg aatggaaaat tttgggcgga aacgcaaaag caaaaagata attttttaat
    15061 gaaattgaaa gcataaaagc gtttttttaa tacatatgcc agcattttaa agaaaccgaa
    15121 aacaccaata aaaatggcga ctaaatttag tcgcctttac tttttcttgc cttgcgtata
    15181 gcctccattc agcaccaaaa cactgtaaga ggctttaatc atggatacct tacccaaatc
    15241 cggctatatg cgcccggcac aggtgataaa ccattttcag attagcgaat cgacgctatg
    15301 gaactgggtt aaaaaaggta aattccccag cccggataag ctgggcgatg gcgtaagccc
    15361 ctttgatgtt tccgctatcc gccagtggct cgctgaccgc aaaggcacga ccagcacgcg
    15421 caattaaccc cgtcagagaa ttttaatcat gaaaatgcca gaaaggcccg caacagggcc
    15481 ggggcgttct cacgcccaaa acaaccacca cacgccccac agaatgggcg gcaaccgcaa
    15541 atattgcttg cctgccatcg ctggtgggcg ttatatttcc ccggctggtg taaatccacc
    15601 agccgggcgt ggaaacccgt ttaatgagca aaaggcgaca cagacgcgcc cagcgtcttt
    15661 ttttgtgtcg tgccatcgcg caaccatgaa cagcggcaag gcccgcactg tatctatggt
    15721 ggcgctggtg gggcagccga aaggctggcc ggtttccttt tgcgccggta tttccacccc
    15781 cgccagcgtc accgcccctt atgagcgtgg aaactcaggc ggtgactcct taaatttgca
    15841 aaaggaggct gccagaatgg ctacgacccc aaccccgtta caccctgaat ttacctttca
    15901 ctttctggcc gttcgccgtt ccgatctgaa cgaccgccca caccgcgaat ccgtcaacgc
    15961 acccgacgaa acaaccgccc gccgtatgct ggcgcgtgat tttgtgttgc tttttgctgg
    16021 ccgcgttccg gctcaggggg tgagccatgc ttaaaaacat gttgatcgcc tcttctttcg
    16081 ggctgtatga tgccggagtc acccacatgg tgaccgggtt tagcagcctg aataacatag
    16141 cggacagccg cttacattcc gatatgcggt ttttttgtgt ccgtaaacct acccataccc
    16201 gcattatggc ggggcgtgac gggggagcct ttgcgctcgc tggtttctat gttgccagtc
    16261 tgctaacccc gttacgtctc gccaccccgt ttagcagcgt agtagcgaga ctccttacct
    16321 caaacatagg agcctttcac atggctgtat tcgcacgccc ttactttgtc tggcgcttta
    16381 tgcagtgcca ggaaaaacaa acctcgctct ttaccgtcac ggcggcaaca gaacgcgaag
    16441 cacgcgcgca gctaccgcac gcgcatatga tttttgtcgc ccgtattcgt cagggggcgc
    16501 gccatgcgtg attgtctgcg tgagtcaatg aaagccgcca tgagcagtat gccggacgaa
    16561 gaaagccact ggattctgcg cgttgatgcc gactggcaca gagtgaattt actggcgggg
    16621 attgcctttg ttggtaaggc actggaggga agccagttac gcgaaaaccc catcacctat
    16681 tccagagacg agatctgcca attagccgga tttctacaga cagcccctgc cctcattggc
    16741 tgtatggccg aactgatgga gtgttacgac cagcaggccg gggaggtaag ccatgtgtga
    16801 tatgtataac acccggatac ctgaactact ggttgcggcc atcaaaaacg cagacgcaca
    16861 ggaagcccgc gccctgtttg atgatgcgga ttactgcgcc cgcaaactgc ttgaagggct
    16921 gattagcaca ggccgcctgc tttccggcat gggcgacaat ctcgacccca gcatgggcga
    16981 actacgcagc ctgggcgatt ccatcgctgt tacggctgaa cttgtggccg gattcagtga
    17041 ggttgtggag gcgtataact ggcgctgccg caccggggaa atcagggagg gcgggcaaca
    17101 tgcgtgacat agccacgatt aacgcaatcc gcagcgttgc cgcagtggcg gcacaacatg
    17161 cggaaatcct tgttcctgaa tggctgcccg aaggtaaacg gcaggccact gaatgggtgt
    17221 gccgtaaccc cacacgtgca gaccgtcacg cgggcagttt tttcgtctcg ctggtggacg
    17281 gatgctggca cgacttcgcc accggggaca gcggcggcga ccttgtggcg ctgggggctt
    17341 atttgtggaa tgtgcgtcag actgatgccg cccgtattgt ggcggacagg ctggggctgt
    17401 gcctgcccgc actgggcaaa cctgatgtaa tgacccgcga acaacgggag gcgcaacggg
    17461 tgcgggtaca ggccgcagaa caggaggcgg gcagactccg ccagcgggag cagcaacaac
    17521 gccaccagcg ccagaaactg accgcctgcc gggcctttga gttgcttggc cgggcggagg
    17581 tggcaacccc ttctcatccc tatctcatga aaaaacggct acagcccggc aatctgtatc
    17641 agtcgggtaa tgatttgctt gtcccgctgt acaacgtatg cggcgaagtg gtgaacgtgc
    17701 agattatcag cccggacggt cgaaaaatgt ttctccgtga tggtcaggtg cagggggctt
    17761 tccatgttat ggggagcttt gacctgatgg ccccggaagc gccggaacat gacgtttacg
    17821 tgtgtgaggg gtgggccacc ggggccgccc ttctccagta ctggaacgtc atggccgtgg
    17881 tgtgtgccat gaatgcggga aaccttaaac atgtggctat ggcgctgcgt gaacgatacg
    17941 gctcgcgcat atctctcgtt atcgccgggg atgatgaccg ggcatcaaaa ggcaaccccg
    18001 gttccagagc agccaacgaa gccgcattgc tggccggatg ttacgtcaca ttcccggaat
    18061 ggccgcccgg tgcatcacca gacctttccg actttaacga tttgatgcta tgggaaatgg
    18121 agcatgaccc ggacaaccac taaaaccccg gtcacatccg ggaagcgaaa accaccacgc
    18181 aagaaacacc ccaaagtcag cccggcacgt ccgggctacg ccacttattc gcagcatgta
    18241 caggtggacg gcggccagat actgaaaccc ggcctttact ggcacggcgt gcgcgactca
    18301 accaacacgg acacgtttat ctgttccccg ctggaggtta tcgccatgac cagcgacagc
    18361 aaagacgaaa actacgggcg attactccgt tttctcacgc cgtcaggcca gtggcgggaa
    18421 tgggccgcac cgatggaaat gctcgccgga gacggcggag aactccgaca ggagctgctg
    18481 cgcatgggcg ttaccattcc ctacaaacaa cggcaggcac tgaccgaata catcatggag
    18541 tcagcaccaa aacgacgggc gctcgcggct acggccaccg ggtggcactc cccgaccctg
    18601 tttgtcatgc cgggcggggt tatcggtgat ggtgatgtgg tgtttcagtc aacagaagca
    18661 ggctcacagg agtacgccac cgcaggaacg ccggagggct ggcgcgacaa cgtggccgca
    18721 ttatgccggg gaaacccggt agccatgctg gcagtgtgtg catcactcgc cgggccgttg
    18781 ctgtggctgc ttgatgtgga cagcggcgga tttcatttca tgggcgacag ttccagcggg
    18841 aaaagcgtgg gggcgctggt ggcctgtagc gtatggggag aaccccgcaa ctttaaacgc
    18901 aactggaacg ccaccagcac cgggcttgaa gggctggcga ccatgcggaa cgataccgcg
    18961 ctgatactgg atgaaattgg cgaagccccg gccagagata ttggcgggct gatttatcag
    19021 cttggcaacg gaacaggccg ccagcgtggc aaggtgtccg gcatggcccg cccggtcaac
    19081 acatggcgca ccatgattat ttccacaggg gaaatgaccg tgggtaaaca catggaaagc
    19141 ggtggtcagc gcatcaaggc cgggcaggaa atgcggttac tggatattcc ggcacagcgg
    19201 ctttacgggg cgtttgactg cctccacgga atggcactac cccacaaccc cgacaacgac
    19261 acagaacgcc gtggagcagg ccggaaattt gccgaaacgc tgcgcggtaa tgcggccacc
    19321 cattacggcc acgccgggcc ggagtttgtg cgctggctga tttcccgcaa tgaaagtgac
    19381 accaccagca ccgacagcaa cggccagacg ctggcggata tgtacgccat actgcgcgaa
    19441 caattccccg taagcaccgg gcaggaggcg cgcgccgccg cgcgtttcac actgtgcgca
    19501 ctggccggtg aaatggcgat aactgccggg attctgccgt ggaaacaggg cgaagcactg
    19561 gaggcggtga aagcgatgtt tgctgcgtgg tcggactatc gtggccgggg gcaatcggag
    19621 gacgtgaaaa tactccgggc cgtcgccggg tttatcgccc gccatgcctc acgctttgaa
    19681 gggggcaaca catacagcga accagtaagc aaccgcgccg gatggtggcg accggggagc
    19741 aacggcgacc gggtttactg gtttttaccg gaagcactat gtgaggcggc cagcggctac
    19801 gacctgaaac gcatagcggc cagtcttgat gatgctggcg cgattgttga ccgcgacacg
    19861 gacgggaaac gccgatacac aaaacgcgtt tccgttaacg gggaaaaaca ccgcgtttac
    19921 gccatcgacc agaatgcatt gttattcgca attgatgaat aattaacaaa aattgcgttt
    19981 ttaaaaactg aaaaagtggt gggactggtg ggaactgtgg gaaccgtaaa aataaaacat
    20041 atataaatca gtaaataata aaatcaccag tatgattatt gttcgttttt gagtgggaac
    20101 tctgcgggaa ctggtgggaa ccacagagaa caaaaaataa ccgccacggt tcccacaaaa
    20161 agccaccgtg ggaactggtg ggaaccactg aaagccgcgt ggttctaggc ggttcccaca
    20221 gttcccacca gtcccaccaa cttgcacaat attacgcacg gcaaaaaacg caccataaaa
    20281 acccggaaat cccttacgtg gcgctggatg caaggcaatt tcagtgtgca aaacgggtta
    20341 aacagcaaaa ccacacagaa tagcgaaaag cccagcaatg gcgcggcatg tggagcattt
    20401 accagtgaat cacgaataac gctttttaag ggcaaaaatg cactttttag cgcacaaaat
    20461 cacgcgaagc cagacgcggc gcggcttaca gccgtgttta tatgcctgat taccgttttc
    20521 tggcggcaaa aaagaacacc gaaacacacg cgcagcccgc gccacacaag ggatacagcg
    20581 aaatgagcac atcagaacac gcagaaatca cgaaaaaaca ccataaaacg cacgttttca
    20641 gcgattcaga acatcagatt tatgcataag ttattcagcc tgccggatgc attgtgtgag
    20701 tttatccggg cattacctgc accggattta caggaagcgc accagtccga aacatggccg
    20761 gaagacaaca ccaccagcaa cggccagacg ctggcggata tgtgcgcccc gtctgcggcg
    20821 tggctggctg cccgtgatgc gtatatcagc cacgtcatga cctgcccgca ttgtgctacc
    20881 agcggcgtta aaaaaccgcg tcattgcgct gagggtgaac gactacggca gaactacgac
    20941 cacaccagat aaccaccaca ccccggcgat taattccggg aacagccggg agaatgcggg
    21001 aaaaggcggg attaatccgg gaataaacgg aaataaccgg gatttcgtgg gactgcaccg
    21061 ccataaggtg gggtgcggtc attattccgc gacgttttcc ccggcaagag cacacgcggc
    21121 gcgggctgca tggaatttca ggccatgcca gaaatgaact ttttcgccca ttgttaccct
    21181 gaatatggta aaaaatgctc acccattgcc gggccatgaa caaaatcagc cccattatgc
    21241 atggcggcca cataccacca gccacatcac agagcgccat tgaatgccag agagtacacc
    21301 acgagcgcca ttttaaaaac cgtttcaaag caccataaaa tcggctttta ggcattgttt
    21361 aagaaagcgt gtttttcaga caggccgacc gactggcagg atatgagaaa ggcaacgtaa
    21421 tcagcgtatc cggcacgatg caggtaagcc agtggaccgg acagaacggg gaaacgcggc
    21481 agggttatca ggtcatagcg gacagcataa tcagtgcacg ggcgacgcgt ccaggcggga
    21541 gcagacgcag taccacaggc acacagagca atcagccacc agcgggaagt tatgacccct
    21601 gcggtgacaa tattccgttc tgagggggtg aacgatggta catgaccgca tagcggaaga
    21661 actggagtcg aaaggctttt accggagagc ggcggcgcga tgggccgaag tgatgatgct
    21721 ggtggagact gacagggaac gcgaacgggt ggcaatgcgc agggcagagt gttacgggaa
    21781 ggcacagagg ccaccggagc cgccagagga gaactacgca gacctgagga aagcagtaaa
    21841 ccgcacttac gcagaaatgg ggataggagg gagatgtaaa aaatctggcg tagctaccag
    21901 aaataagcac agagccggaa cagtcggtta gcctttctga tatgtgtgcg tgtatatcgt
    21961 tttatcgcga cgttaaatta aactggtagg accagataca ccaacacaag aggctaccgg
    22021 atgaacgagc aaacccatca cgtaccaaaa gacatcgagc ggcttaaatt catgaatgac
    22081 atagacggcg gagataaacc aggtgatgca tggtgtgatt cgcttgctga tatcgtgtcg
    22141 cattcagtgg cagaacttac ccgtaatggc atggaggagg cagacgcttt cagaatcgca
    22201 aagcgggttg ttgctgcaat cgccttctat gctggtgggc ggagtcttta ccttccaaca
    22261 ggcagatcac ttttcgcagc attgcgcgaa tacgagattt actacaggtg ggataacggc
    22321 gaaaaattgc aggcattacg taaagagtac gcgatatccg agacccatat ttacgatatc
    22381 attcggcggc ttagaaagaa gcacgccaat aaaatcacgc aagactaacc acagatacca
    22441 gcagcaaccg ttatatttgc gccgctatcg caaaaatccc ctttcagggc atcacagaca
    22501 ccacaaaaca ccaaaacgca tcagagagcg aaaggcgctt taatgataat ttgttttttg
    22561 tgaaagaagg tgaaagacgg tgatttttgt gaaattaagc gaaatcacgt gaaatcaggt
    22621 gaaataatga tacccgttca tctggtgcta ccagacaaaa cggactttac ccgcgcataa
    22681 cgcataatta ctattttgcc tggctgtatg gtgtactctt aatcggtaaa gcggcataaa
    22741 ccctaacggg ccatatgttt atgtgggttc gacccccacc accagcagcc aacaacctca
    22801 tcgctttttt gctggtgtac gctcaacggg tagagtcggt gccagcctgt acaggctgat
    22861 catgtatgcg ggttcgaccc ccgccaccag cacgccaaaa gcacaattgc gccatttcta
    22921 aacccacttt aaagccacat aaaaacggct ttcgtatccc gttttattga gcactcacga
    22981 aaaatgtgta catacgcgtg tacatataca aattaaccca acccaaagaa ccgcataata
    23041 aaagcaatta acgcacatac taaacattca gattcaagag taactcgtaa cactaattct
    23101 tacttttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt cttccccgca
    23161 acttcaattc tgttttgcgt gcctgctcca gattttgcga tgtttttttg cccagcacac
    23221 tgagaacgtg agatactcac aactgtatat aaatacagtt acagatttac tttctttgca
    23281 atcgatatca catggagtgg gcaatgaacg aacatcatca accttttgaa gagataaaac
    23341 tgattaatgc aaacggagca gaacaatggt cagcaagaca acttgggaaa ctactgggtt
    23401 attcagagta tcgtcacttt atacctgtat taacgcgcgc caaagaggcc tgtgagaaca
    23461 gtggtcacac aattgatgac catttcgagg agatcctcga tatggtcaaa attggctcaa
    23521 atgccaaaag agcattaaaa gacatcgtac tctcccgcta tgcctgttac ctggtagtac
    23581 aaaacggcga ccccgcgaaa ccggtcattg cggcagggca gacttatttt gctatccaga
    23641 cccgacggca ggagcttgct gacgatgaag catttaagca acttcgtgaa gacgaaaagc
    23701 gtctgtttct gagaaatgag ttgaaagaac ataacaaaca attggttgag gcagcacagc
    23761 aagctaatac cacacacttt gacgtgggta gcaaggtgag gcaaaccatt caggaacttg
    23821 gcggaaccat gcctgaggag ttgccgaccc cgcaggtcag catcaagcag ttggaaaaca
    23881 gtgtaaaaat tacagagaag aaatagcaga aacgaaaaac tctttttgtt gattgagaca
    23941 cccgatgcgt aagccaaggt ccaggtgcag tgaacacaat ggctaaacat tgcacctttc
    24001 ttttcccccc tcagttttaa cctatttttt cttatgcatt ttctcagaca agaagtcaga
    24061 agaatgcatc tacgctacag aaaatagcga tttcacataa ctacaagtta tcaatttcct
    24121 ctcaccttaa aaaatctcaa tcgtgacaat acgcacaaat cgctaccctg ccagacagat
    24181 ttttagggag agaaccatgc tgttacacat tttgtatttg gttggcatta ctgccgaagc
    24241 catgacgggg gctctggcgg ccgggcgacg ccgcatggat acatttggcg taattattat
    24301 tgcgaccgcc accgcaattg gcggagggtc agtgcgcgat attctgctgg gccactatcc
    24361 gctcggctgg gtcaaacacc cggaatatgt gattatcgtc gctaccgccg cagtactcac
    24421 cactatcgtt gctcctgtaa tgccttacct gcgcaaagtg tttctggtgc tcgatgcgct
    24481 tggactggtg gtcttttcta tcatcggcgc acaggttgca ctggatatgg ggcacggccc
    24541 aattattgcc gttgtcgcgg cggtaaccac cggcgtgttt ggcggcgttt tgcgcgatat
    24601 gttctgtaaa cgtatcccac tggtattcca gaaagagttg tatgccggtg tctcatttgc
    24661 ctccgccgtg ctgtacatcg cgctgcaaca ctatgtttct aaccatgatg tggtaattat
    24721 ctccaccctg gtattcggct tttttgcccg cttactggcg ctacgcctta agctgggatt
    24781 accggtcttt tactacagcc acgaaggaca ctaaggttca aaacctgtga tctgctgggc
    24841 agccagccaa ctgcccagct tcttgatttg cgcattttcc ttccattcaa taacctgtct
    24901 ggcgcgcccc gatccagtcc ccggcagctg ctgccagaac tgctccgtgc taaataaaag
    24961 ttgcgaccag gaccgttcat cactggcatt aagcgccgcc cgggttagcg gtattcccat
    25021 tgccatcacc cagcgagtaa aaggctgctt acgagccaga ttaaactgat gccatagctg
    25081 cgcactttta cttttcgcga tccccggcgt gttctgtaat tgctctggcg ttaataaaag
    25141 ccaggaaaag atatgctcaa agcgatgagt ctgatgcagc gcgcgccagc cggcctcacc
    25201 aatgccatcc agccccagaa cctgttttga ccccagccag actaagcgtg aaatgaactg
    25261 ttcctgacaa acatcagaag caaagtagca ggtcaacgag ttaaagcggt tttctggtgg
    25321 tgtcggtttt gtacgttctg caccgcgcca gaccacatca tcaatgcgag gaataccctg
    25381 accggcaagg ctgacgagga tctgatcacc aggcgcaata tcccactcct gccagcgcct
    25441 gacggaacca atattcaccc gctggacttt tttatcatcc agcatgacag gtgcgagtga
    25501 cgcaaccacc gatattttac cgctcttacc caccgcaaac tgaatcgcct tcacttcggc
    25561 aacctgagct acaggttgat atttccaggc caccagccat tctgcctggc ctggtagcca
    25621 atggcgggat tcgggttctt tcgcccctcg cacaaccacg ccatcggtga cgaagggtaa
    25681 tttcgctttc caccactcat tgcgtacgcg cgcaacctca tcagcatttt taaccgcacg
    25741 ggtatacctc tgcgttagag taaaacctgc ggtagccagc tcctttaaac gatcggtcat
    25801 taactgcggt ccatccggcc atgcccagac aaaaacgccc agagaattca gcgtgtcgct
    25861 atcgtcctgg cgcatcatca ggccagcaac ttttgcgcgg gcatttattc cccccatttg
    25921 ttgttggata tgcccctcgc gctggagaaa tatttccccc tgaagcgtac tgttggctaa
    25981 aggcccgcta acggtttgcg gcacagcgga aattaagcta actttctgcg tccagtcctc
    26041 gcctttcagg ccattaccgc gactgattgc tttgttcagt ttcccgtccc gataaaccag
    26101 ggttaccgca acgccatcaa cttttggctg cacccaaaga tcgctacgtt ctcgcatcca
    26161 cagacttaat gcgtttttat ccgccatttt gcgtacaccc gtatgggcaa ccggatgcat
    26221 aaccgcacca tttaaaggcg gcatcatcac gtcgcggggc tcgctcacaa agcagcgttg
    26281 ccactgcgta agacgggcgc ttaactgatc gtaaacaccg tcttccacct cactttttcc
    26341 ttccttccag tagtcatcgt cccactgttt tatttgctgt tgcaggcggg aaatttcttc
    26401 ctgtgctctg gctggcgacc aggccggaca gaccgcccac acagatgatt gccagcacaa
    26461 gatacttatt aatatcgcca tccatacttt catcttcacc tccgctgtag tcaggctggc
    26521 agatatacaa cgtggtgaag gcaaagccga gtggcaaaaa cggagtctgc gaggacgctt
    26581 cctgagaatc gtctttattg cagtaaatca caggcaaatg cggaaacagc tacgcaaaac
    26641 gcaacaactt tgcgcaaaaa gtgtgagcaa gggctacgtc acatggccgc gccgtgtata
    26701 ataagctcgt atgtaggctt tatttcgcta atcacatacg aaagatactc atggctcaag
    26761 gcacgcttta tattgtttct gcccccagtg gcgcgggtaa atccagcctg attcaggctt
    26821 tattaaaaac ccaaccgttg tatgacaccc aggtttctgt ttcacacacc acacgccaac
    26881 cgcgtcctgg tgaagtccac ggtgaacatt atttctttgt taatcatgat gaatttaaag
    26941 aaatgattag cagagatgcg ttcctcgaac acgcagaagt ttttggtaat tactatggca
    27001 cttcgcgtga ggccattgag caagtactgg cgaccggtgt cgatgttttt ctcgatatcg
    27061 actggcaggg cgcgcagcaa attcgccaga agatgccgca cgcgcggagt atctttattt
    27121 taccgccgtc caaaattgaa ctggaccgcc gtctacgcgg tcgcggtcag gacagcgaag
    27181 aggtcattgc aaagcgtatg gcgcaagctg ttgcagaaat gagccattac gccgaatatg
    27241 attatctgat tgtgaatgat gacttcgata ccgcgttgac cgatttgaag accattattc
    27301 gcgccgaacg tctgcgcatg agccgccaaa agcagcgtca tgacgcttta atcagcaaat
    27361 tgttggcaga ctgaacctga tttcagtatc atgcccagtc atttcttcac ctgtggagct
    27421 ttttaagtat ggcacgcgta actgttcagg acgctgtaga gaaaattggt aaccgttttg
    27481 acctggtact ggtcgccgcg cgtcgcgctc gtcagatgca ggtaggcgga aaggatccgc
    27541 tggtaccgga agaaaacgat aaaaccactg taatcgcgct gcgcgaaatc gaagaaggtc
    27601 tgatcaacaa ccagatcctc gacgttcgcg aacgccagga acagcaagag caggaagccg
    27661 ctgaattaca agccgttacc gctattgctg aaggtcgtcg ttaatcacaa agcgggtcgc
    27721 ccttgtatct gtttgaaagc ctgaatcaac tgattcaaac ctacctgccg gaagaccaaa
    27781 tcaagcgtct gcggcaggcg tatctcgttg cacgtgatgc tcacgagggg caaacacgtt
    27841 caagcggtga accctatatc acgcacccgg tagcggttgc ctgcattctg gccgagatga
    27901 aactcgacta tgaaacgctg atggcggcgc tgctgcatga cgtgattgaa gatactcccg
    27961 ccacctacca ggatatggaa cagctttttg gtaaaagcgt cgccgagctg gtagaggggg
    28021 tgtcgaaact tgataaactc aagttccgcg ataagaaaga ggcgcaggcc gaaaactttc
    28081 gcaagatgat tatggcgatg gtgcaggata tccgcgtcat cctcatcaaa cttgccgacc
    28141 gtacccacaa catgcgcacg ctgggctcac ttcgcccgga caaacgtcgc cgcatcgccc
    28201 gtgaaactct cgaaatttat agcccgctgg cgcaccgttt aggtatccac cacattaaaa
    28261 ccgaactcga agagctgggt tttgaggcgc tgtatcccaa tcgttaccgc gtaattaaag
    28321 aagtggtgaa agccgcgcgc ggcaaccgta aagagatgat ccaaaaaatc ctctctgaaa
    28381 tcgaagggcg tttgcaggaa gcgggaatac cgtgccgcgt cagtggtcgc gaaaagcatc
    28441 tttattcgat ttactgcaaa atggtgctca aagagcagcg ttttcactca atcatggaca
    28501 tctacgcttt ccgcgtgatc gtcaatgatt ctgacacctg ttatcgcgtg ctgggccaga
    28561 tgcacagcct gtacaagccg cgtccgggtc gcgtgaaaga ctatatcgcc attcctaaag
    28621 cgaacggcta tcagtcgttg cacacctcga tgattggccc gcacggcgtg ccggttgagg
    28681 tccagatccg taccgaagat atggatcaga tggcggagat gggtgttgcc gcgcactggg
    28741 cttataaaga gcacggcgaa accagtacta ccgcacaaat ccgcgcccag cgctggatgc
    28801 aaagcctgct ggagctgcaa cagagcgccg gtagttcgtt tgaatttatc gagagcgtta
    28861 aatccgatct cttcccggat gagatttacg ttttcacacc ggaagggcgc attgtcgagc
    28921 tgcctgccgg tgcaacgccc gtcgacttcg cttatgcagt gcataccgat atcggccatg
    28981 cctgcgtagg cgcacgcgtc gaccgccagc cttacccgct gtcgcagccg cttactagcg
    29041 gtcagaccgt tgaaatcatt accgctccgg gcgctcgccc gaatgccgct tggctgaact
    29101 ttgtcgttag ctcgaaagcg cgcgccaaaa ttcgtcagtt gctgaaaaac ctcaagcgtg
    29161 atgattctgt aagcctgggc cgtcgtctgc tcaaccatgc tttgggtggt agccgtaagc
    29221 tcaatgaaat cccgcaggaa aatattcagc gcgagctgga tcgcatgaag ctggcaacgc
    29281 ttgacgatct gctggcagaa atcggacttg gtaacgcaat gagcgtggtg gtcgcgaaaa
    29341 atctgcaaca tggggacgcc tccattccac cggcaaccca aagccacgga catctgccca
    29401 ttaaaggtgc cgatggcgtg ctgatcacct ttgcgaaatg ctgccgccct attcctggcg
    29461 acccgattat cgcccacgtc agccccggta aaggtctggt gatccaccat gaatcctgcc
    29521 gtaatatccg tggctaccag aaagagccag agaagtttat ggctgtggaa tgggataaag
    29581 agacggcgca ggagttcatc accgaaatca aggtggagat gttcaatcat cagggcgcgc
    29641 tggcaaacct gacggcggca attaacacca cgacttcgaa tattcaaagt ttgaatacgg
    29701 aagagaaaga tggtcgcgtc tacagcgcct ttattcgtct gaccgcccgt gaccgtgtgc
    29761 atctggcgaa tatcatgcgc aaaatccgcg tgatgccaga cgtgattaaa gtcacccgaa
    29821 accgaaatta atgttttatg aacccaacac gttatgcacg catctgcgaa atgctcgcca
    29881 ggcggcagcc tgatctgacc gtctgcatgg agcaggtcca caaacctcat aacgtttctg
    29941 cgattattcg taccgcagat gccgttggcg tacatgaagt tcacgccgtc tggcctggta
    30001 gccgcatgcg caccatggct tcggcagcgg cgggtagtaa cagctgggta caggtgaaaa
    30061 cacaccgcac cattggcgat gccgtcgctc atctcaaagg ccagggcatg cagattctgg
    30121 caacccatct ttctgataac gctgtcgatt tccgcgaaat tgattacact cgcccgacct
    30181 gcattttgat gggacaggag aaaacgggca tcacgcagga agcattggcc ctggcggatc
    30241 aggacatcat cattccgatg atcggcatgg tgcagtcgct gaatgtttcc gttgcctcag
    30301 ccctcattct ttacgaagcc cagcgtcagc ggcaaaatgc aggcatgtac ctgcgtgaaa
    30361 acagcatgtt gccggaagca gagcaacaac gcctgttgtt tgaaggcggc tatccggtgc
    30421 tggcgaaagt cgcaaaacgc aaaggcctgc cttatcccca cgtcaatcag caaggcgaga
    30481 tcgaagctga tgccgactgg tgggctacta tgcaggctgc agggtaagtg ccatgaaagg
    30541 tcgcctgtta gatgctgtcc cactcagttc cctaacgggc gttggcgcag cacttagtaa
    30601 caaactggcg aaaatcaacc tgcataccgt gcaggatcta ctcttacacc ttcccctgcg
    30661 ctacgaagat cgcacccatc tctaccccat cggagaacta ctgccgggcg tttatgccac
    30721 ggtggaaggc gaagtgctga actgcaatat ctccttcggc ggtcggcgga tgatgacctg
    30781 ccagatcagc gacggttccg gcatcctcac catgcgcttt ttcaatttca gcgcggcaat
    30841 gaaaaatagc ctggcgacgg gccgccgtgt actggcttat ggcgaagcaa agcgcggtaa
    30901 atatggtgcg gagatgatcc acccggaata ccgcgtgcag ggcgatctca gcacgccaga
    30961 attacaggaa acgctcacgc cggtttatcc aacaacggaa ggcgtaaagc aggccacgct
    31021 gcgtaaatta accgaccagg cgctggatct gctcgacacc tgcgccattg aagaactcct
    31081 gccgccggaa ctgtcacaag gaatgatgac gctaccggaa gcgttgcgca ctttgcaccg
    31141 cccgccaccg acgctacagc ttagcgatct ggaaaccggg cagcatccgg cgcaacgtcg
    31201 tctgattctg gaagaactgc tggcgcacaa cctcagcatg ttagccttac gtgccggagc
    31261 acagcgtttt catgcccagc cgctgagcgc caatgacacg ctgaaaaata aactcctcgc
    31321 cgccttaccg ttcaagcaaa cgggcgcaca ggcacgcgta gtggcggaga tcgagcgcga
    31381 tatggcgctg gatgtgccga tgatgcgtct ggtgcagggc gatgtaggtt ccggtaaaac
    31441 gctggtcgcc gccctcgccg cgttgcgtgc gattgcccac ggcaaacagg tagcattgat
    31501 ggcaccaacc gaattactcg ccgagcagca cgccaataac ttccgcaact ggtttgcacc
    31561 gctcggtatc gaagtgggct ggctcgccgg taagcagaaa ggtaaagcac ggctggcaca
    31621 gcaggaagcc atcgccagcg gtcaggtgca gatgattgtc ggtacacacg ccatcttcca
    31681 ggaacaggtg cagtttaacg gcctggcgct ggtgattatc gacgaacagc atcgttttgg
    31741 cgtacatcag cgtctggcat tgtgggagaa aggccagcag cagggcttcc atccgcatca
    31801 gttgatcatg accgccacgc cgatcccccg cacgctggca atgactgcgt atgccgatct
    31861 cgatacctcg gtgatagatg agctgccgcc aggccgcacg ccagtgacta cggtcgctat
    31921 tcctgatacc cgccgtaccg acatcattga ccgcgtgcgc cacgcctgca taactgaagg
    31981 tcgtcaggca tactgggttt gtacgttgat tgaagagtcg gaattgctgg aagcgcaggc
    32041 ggcggaagct acctgggaag agttgaaact ggcgctacca gagttgaacg ttggcctggt
    32101 acacgggcgg atgaaacctg ccgagaaaca ggcggtgatg gcgtcgttta aacaaggtga
    32161 gctacacctg ctggttgcca caaccgttat tgaagtcggc gttgatgtgc ctaacgccag
    32221 tctgatgatt atcgaaaacc cggagcgtct gggtctggcg cagttacacc agcttcgcgg
    32281 gcgtgtaggt cgtggtgcgg tggcttctca ctgcgtgctg ctctacaaaa cgccgctctc
    32341 caaaaccgcg caaattcggc tgcaagtgct gcgcgacagc aacgacggtt ttgtgattgc
    32401 acagaaagac ttagagattc gcggaccggg tgaactgtta ggtacgcgtc agacgggtaa
    32461 tgctgagttt aaagtggcgg atttactgcg cgatcaggcg atgatcccgg aagttcagcg
    32521 cctggcgcga catattcacg aacgttaccc acaacaagca aaagccctga tagaacgctg
    32581 gatgccggag acggaacgtt actccaacgc atgaccagac tcatcacaag acaagtcacg
    32641 cagtaaatct tcaatctgga tagtgaggat atccattgcc tcatacgcat cctgtgcaat
    32701 tcgcgccgcc attgcgcctt ccggttcacc acgcagaatt tcagccgctg cctgtacgcc
    32761 agccacaatc atttcaggtg ttaacatttg gcgaagaaaa gcgcgattat cagaggacca
    32821 gccatcttta tggcctaatg gatgccccca gcctatccat tcttgcgacc ataacggatt
    32881 ggcttctatg ggataaatta gatgcgcggc atactgaata gcacacaccg cttttcctgg
    32941 cgtatccagc gcccaccagc gcgaccagag agaacgaatt aacggcgcgg ccccacccag
    33001 agtattgaac accgccagcc agcaaagaac gttattgaaa ccacgctcgt tatccatacg
    33061 ctgcaacgcg gtgtccagta aaaattgccg tacctgctgg cgttgggaag cattcatcat
    33121 ctcctgccag agatagggcc gacacaatgc cttcattaag gattcttcga agtgatcacc
    33181 ataaccgcca gctaaaacgg tttcatgcca ttgcgccagg cagagcggaa acagatagcg
    33241 aaacaactct atttgcatcg gttcgttata aacgagattg agtacatagt aagcactcaa
    33301 atcccattcg ctgatctgct gccactcttt agccgccagc gccttgagtt cggcatctgc
    33361 gtaatcaaat tccgtttccc atacacagag cactggcggc tcatcaccaa aaatatggcg
    33421 acagtgttca cgtagcgttt ccggttcctt ccgacgcttt ttcattttta accggcaaaa
    33481 atcggcaaca ttaaatacag cttaatcacc agcgcattga cgatatcgat aaagaacgca
    33541 ccgaccatcg gcaccaccaa aaacgccatg tgcgacgggc caaagcgttc agtgatcgcc
    33601 tgcatgttgg cgattgccgt tggcgttgca ccgaggccaa aaccacagtg acccgcagcc
    33661 agcactgccg catcgtagtt tttgcccatc atgcgccagg taacgaagat ggcatacaac
    33721 gccatgaaga tggtctgtac caccagaatc gccagcatcg gcagcgccag cgaagccagc
    33781 tcccacagtt tcagccccat cagcgccatc gccaggaaca acgacaggct tacgttaccc
    33841 agcacggata ccgcacgctc aaagacgcgg taaaagccca tcattgacag accgttgctc
    33901 agaatcacgc caacaaacag tacacagacg aaggtcggca gttcaaaagc agtgccagcc
    33961 aaaagttgcg caacaatttt ccccaccgtc aggcagatag caatcagcgc gatagtttca
    34021 atcagcacca gcgaggtgat catgcgtccc acatccggct tttcaaacgc cgtcgggact
    34081 tcctggtcat ccggaatacc gttcggcgtg gtggagtgtt tcaccagata gcgcgccacc
    34141 ggaccgccaa tcaagccgcc cagcaccaga ccgaacgttg cacaggccat cgccacttcc
    34201 gtcgcattgg tgaagccata acgttcaatg aacaatttac tccacgcagc gcccgtaccg
    34261 tgaccgccgg aaagcgtaat agaaccggcc aacagcccca tcagcggatc aagccctaac
    34321 aggctagcca taccaatgcc aatggcattt tgcatcacca acagaccaac aaccacaatc
    34381 aagaagatgc caaccacacg cccaccggca cgcaaactgg caatgttggc gttcaggcca
    34441 atggtggcga agaaagccag cattaacgga tcgcgcaggg acatatcaaa gttgacttcc
    34501 cagcccatgc tttttttcag tactagtagc gccagcgcca ccaacaaacc acccgcaaca
    34561 ggttccggta tggtgtattt cttcaaaaag gagacggaat ggaccaactt acgcccgagc
    34621 agcaacgtca gcgttgcggc aacaagcgtt gctaaagtat cgagatgaaa catagttact
    34681 cctttgtatc cgcatcactc tttcatactc gtcatacttc atcgcgcagt ggcaacttca
    34741 tgaactatga cgggtatcga gttatgcttt cctgggtcaa attctcagct gttaatgaac
    34801 aaatggcatg gattttaagc agaaaaatac caaaagttat ataaaaaagt gcatttatct
    34861 catttaaata tttagatgag gatgtggcaa acgtttgctt ttctctttcg gtcagctaaa
    34921 atgcccgctt tgctaccacg ggattgtttt cgatgtctgt ttccaccctc gagtcagaaa
    34981 atgcgcaacc ggttgcgcag actcaaaaca gcgaactgat ttaccgtctt gaagatcgtc
    35041 cgccgcttcc tcaaaccctg tttgccgcct gtcagcatct gctggcgatg ttcgttgcgg
    35101 tgatcacgcc agcgctatta atctgccagg cgctgggttt accggcacaa gacacgcaac
    35161 acattattag tatgtcgctg tttgcctccg gtgtggcatc gattattcaa attaaggcct
    35221 ggggtccggt tggctccggg ctgttgtcta ttcagggcac cagcttcaac tttgttgccc
    35281 cgctgattat gggcggtacc gcgctgaaaa ccggtggtgc tgatgttcct accatgatgg
    35341 cggctttgtt cggcacgttg atgctggcaa gttgcaccga gatggtgatc tcccgcgttc
    35401 tgcatctggc gcgccgcatt attacgccgc tggtttctgg cgttgtggtg atgattatcg
    35461 gcctgtcgct aattcaggtt gggttaacgt ccattggcgg cggttacgca gccatgagcg
    35521 ataacacctt cggcgcaccg aaaaatctgc tgctggcagg cgtggtctta gccttaatta
    35581 tcctgcttaa ccgtcaacgt aacccttact tacgcgtggc ctcactggtg attgcgatgg
    35641 cggccggata tgcgctggcg tggtttatgg gcatgttgcc agaaagcaac gaaccgatga
    35701 cgcaagaact gattatggtg ccaacgccgc tctattacgg tcttggcatt gaatggagtc
    35761 tgctgctgcc gctgatgctg gtctttatga tcacttcgct ggaaaccatt ggcgatatca
    35821 cggcgacctc tgacgtttcc gaacagccag tgtccggtcc gctgtacatg aaacgcctga
    35881 aaggcggcgt gctggcaaac ggcctgaact cgtttgtttc ggcggtgttt aacaccttcc
    35941 cgaactcctg cttcgggcaa aacaacggag tgatccagtt gactggtgtt gccagccgct
    36001 atgtcggttt tgtcgtcgcg ctgatgttga tcgtgctggg tctgttcccg gcagtgagcg
    36061 gttttgtaca acacattcca gaaccggttc tgggcggcgc aacgcttgta atgtttggca
    36121 ccatcgccgc ctccggtgtg cgtatcgttt ctcgtgagcc gctgaaccgt cgggcgattc
    36181 tgattatcgc gctgtcgctg gcggttggtc tgggcgtgtc tcagcagccg ctgattttgc
    36241 agtttgcccc tgaatggctg aaaaacctgc tctcctccgg gatcgccgcg ggcggtatta
    36301 ctgccatcgt gctgaatctg attttcccac cagaaaaaca gtaattcact ggcggcggta
    36361 agataattac cgccgcagtc tcacctcttt caccattccc gccttgagga ttgcgcgtaa
    36421 atcgtgcata actcccttat gtgcatttca cgggatggaa gaccatgaaa tttattggga
    36481 agctgcttct ctacattctc atcgctctgt tagtggtgat cgctggcctc tattttcttc
    36541 tgcaaacccg ctggggagca gaacatatca gcgcatgggt ttccgagaat agcgactatc
    36601 atctggcctt cggggcgatg gatcaccgtt tttccgcgcc atctcatatc gtgctggaga
    36661 acgtcacgtt tggtcgtgat ggtcagcccg cgaccctggt ggcaaaaagt gtcgacattg
    36721 cgctaagcag tcggcaactg accgaaccac gccatgtcga taccatcctg ctggaaaacg
    36781 ggacgctgaa tctcaccgac cagaccgcgc cgctaccgtt caaagccgat cgtctgcaac
    36841 tgcgtgatat ggcgtttaat agcccgaata gcgaatggaa actgagcgcg cagcgggtaa
    36901 atggcggcgt ggttccgtgg tcaccagaag ccggtaaagt gctgggtacg aaggcgcaga
    36961 ttcagtttag tgccggatcg ctttcgctca atgatgttcc tgccaccaat gtactgattg
    37021 aaggcagtat tgataacgat cgcgttacgc tgactaacct gggtgccgac atcgcccgcg
    37081 ggacattaac cggaaacgcg cagcgtaacg ccgacggcag ctggcaagtg gaaaatctgc
    37141 gcatggcgga tatccgtcta caaagcgaaa aatcgctaac cgacttcttt gcgccattac
    37201 gctctgtccc gtcgttgcag attggtcgcc tggaagtgat cgatgctcgt ttgcaaggtc
    37261 cggactgggc ggtgaccgac ctcgatctca gcttgcgcaa catgaccttc agtaaagatg
    37321 actggcagac acaagaaggc aaactgtcga tgaacgctag cgagttcatt tatggttcgc
    37381 tgcatttatt tgacccgatt ataaacgcgg aattttcccc gcagggcgta gcgctgcgcc
    37441 agttcaccag ccgctgggaa gggggtatgg tcagaacgtc agggaactgg ctgcgtgacg
    37501 ggaaaacgtt gatccttgat gatgcggcaa ttgccgggct ggaatatacc ttgccgaaaa
    37561 actggcaaca gttgtggatg gaaacgacac ccggttggtt aaacagcctg caactgaaga
    37621 gatttagcgc cagccgcaat ctgatcattg atatcgaccc tgacttcccg tggcagctca
    37681 ccgcgctcga tggttacggt gccaacctga cgctggttac cgatcataaa tggggcgtct
    37741 ggagtggctc ggcgaatctg aatgccgccg ccgcgacatt caatcgtgtt gatgttcgtc
    37801 gcccgtcgct ggcgctgacc gccaacagca gcacggtgaa tatcagcgaa ctgagtgcat
    37861 ttactgaaaa aggcattctg gaagccaccg ccagtgtttc acaaacgcca caacgtcaga
    37921 cacatatcag cctgaatgga cgcggtgtgc cggtgaatat tttgcaacag tggggatggc
    37981 ctaaattacc gttgactggc gacggcaata ttcagcttac cgccagtggc gatattcagg
    38041 ccaatgtccc gttgaaacct acggttagcg ggcaattgca tgccgtgaac gccgcaaagc
    38101 agcaagtgac tcaaaccatg aatgctggca tcgtttccag cggtgaagtt acatcgacgg
    38161 agccggtgcg gtaaacatca atctcgccgg gtacgactca gacgtacccg gcattccatc
    38221 aatagattac aacgtaattg tcagcgcatt cccttgaggc ttcaccacca gcccctgctc
    38281 actttcagcc tgcgaaccgt cttgcagacc atttactttc acaacattgc gcaggcacag
    38341 cgtccagttc ttcgcctcgc ccgcaccagt cacagtaatc gtgttgccag tacgtgctgc
    38401 ttttaaagta aagatcaccg atccgtcagt agcgggcact tcacagacgg cttcatgccc
    38461 gtcttgcaga ttaaagaggt ggaatgccgt gccttcgtgc cacacgtaat cgggacgttg
    38521 atcgttgttg cccagcgcca gtagagtgtt atcacgcaca taaacgggca gactcaggaa
    38581 gccgtgctgc tgtttatgcc agcgactacc gtcgagttca tcgttgtgcc acaggtgtgt
    38641 ccagcgacct tccggcaggt agaactgcac atcgcccgct tcagtgaaca ccggcgcaac
    38701 catcacgttg tcgcctaaca tgtattgacg gtcaaggtaa tcacaagccg gatcgtccgg
    38761 gaattccatc atcatggccc gcatcatcgg cgtaccccgc gcgttcgcac gcgcagcttc
    38821 acgatacaga tacggcatca tgcggcattt cagttgcgtg aagaagcgca ccacatcaca
    38881 ggactcatca tcgtaggccc acggcacacg ataagatttg ctaccgtgta aacggctatg
    38941 gctggagagc aaaccaaacg cgcaccagcg tttgtaaacg tgcgccggag cggtattttc
    39001 aaagccgccg atatcgtggc tccagaagcc aaaacctgaa aggccaatag acaaaccacc
    39061 gcgcaggctt tccgccattg attcgtagtt agcgtaacaa tcgccacccc agtgtaccgg
    39121 gaatttctgc gcaccgacgg aggccgagcg ggcaaacaag acagcttctt cctcaccaac
    39181 ggtgtccttg agcacgttcc acaccagttc gttgtagatg tacgcataat ggttatgcat
    39241 tttctgcgga tcggaaccgt caaaccactg aacatcagtt gggatacgtt cgccaaagtc
    39301 ggtcttaaag caatcaacgc ccatcgcgac cagacctttc agtttgtcgg cgtaccattt
    39361 gcaggcatcc ggattggtaa agtcataaat cgccagacct ggctgccatt tatcccactg
    39421 ccatagcgaa ccgtccgggc gtttgagtaa atagcctttc tcttgtaact ctttaaagac
    39481 gggggatttt tgaccgatat aggggttaat ccagacgcag attttcagtc ctttcgcttt
    39541 caggcggcgg atcatccctt ccgggtcagg gaaagtcagc gggtcccact caaaatcgca
    39601 ccactggaag gctttcatcc agaaacagtc aaagtggaaa acatgcagcg gcagattgcg
    39661 ttccgccata ccatcgataa agctgtttac cgtcgcttcg tcgtagttgg tggtaaatga
    39721 agtggttagc cacaggccga aggaccacgc gggcggcagc gccggacgac cagtaaagcg
    39781 ggtataacga tcaagtaccg ctttcggcgt cgggccgtcg ataacaaagt attcgagata
    39841 ttcactctca acgctgaact gcactttgga gactttctcc gatcccactt caaaagagac
    39901 acactgggga tgattgacca gtaccccata accacggtta gtcatgtaga acgggatatt
    39961 tttatacgcc tgttcagtac ttgtgccgcc gtcccggttc caggtctcta ccgtctggcc
    40021 attgcgcacc agggcagtaa agcgctctcc cagaccgtaa actgtttcgc caacgccaag
    40081 atcaagccgc tcaaacatat aattgcgttg attattcgtg tcctgcacgt agccattatt
    40141 tttcacctga ctaccggtaa tacgttcgcc gttgcgcaga aaatccagtg accagaactc
    40201 acctttgctg acacgcgcgc ttaagttgcc acttttaaac tcagcataac gttctgtgtt
    40261 ttcgattgtg accttcacgt cctgcaaaat attgagcgga taatgaggac cgttattcag
    40321 cgccccctga aaatgctcaa tccgcacacc gacaatacct tcctgtgggg agaaaaagcg
    40381 caacgtaaat aaaggcgtat caagctgcca ggtacgttca cgcacatcac ggggggcagc
    40441 atagaccacc atttcattat cctgctgttc aacctcgaac acctgaagcg ggtgaatcaa
    40501 attgaggcca ggttgaatca accagtttcc atcgctaatt ttcattgccg ttccttagtt
    40561 ctgcacttct tgagaggtga attgttgctg gcaacggcgt ttaccctgag ccagctgttc
    40621 cataacggtt ttcagattgt gcgtcgtgag tgagtagtag cgtttagcga taatcgcgct
    40681 cagcaaataa cagatcgccg gaacaatcgt gaatagcgca ataatgatgc taatcgtggc
    40741 gctgttctgc gctttttctg ccgcatcata tccgccataa gccagcatcc agccgataag
    40801 agcgccgccg aaggccaacc ccagtttgag cacaaacagc gtgccagcaa aactgatccc
    40861 ggtcaggcgt ttaccattgc accactcgcc gtagtcgacg gtatcggaca tcattaccca
    40921 ctggataggt gtcaccagtt gatgcaacac accaatcacg aagatgaaga caaacatagt
    40981 gatgctggcc tgcatgggaa caaagaacat cgcgaggcta atcactgcca gcagggcgtt
    41041 cgtccaccag aagatagtga ctttacattt ccagtcggtc agaggttttg ccagtgcgga
    41101 accaatcagg ttacccacgc aataagtggt gagaaaagcg acaaacactt ccggcgtgcc
    41161 caaaatccat gtgacgtaat acatcatcgc cccaccgcgt acgcacaccg ccaggatatt
    41221 gaaaatggtt agtaaaccga caatccgcca ctggtcgttt tgccagatat cacgtaaatc
    41281 ttcccgcata gacgttgttg taggtggtgc ttcaacgcgt tctttagtgg tgaagaaaca
    41341 gaatgccagc atcatgaatg ccaccacgga aaggaccgcg ataccgccct ggaaaccgag
    41401 tggtttatta tcaccgccaa ttaaattaac cagtggcatc atcagaacag tagaaagcat
    41461 gcctcccgcg gtcgccagca caaaacgcca ggattgcagc gagatacgct gagtcgggtc
    41521 attggtgatt acaccaccca atgcgcagta agggatattg acgacggtat aaagtaaggt
    41581 aagtagggtg taagtaattg ctgcatagat cattttgccg ttcatactga gatctggcgt
    41641 gctataggcc agtacacaga cgatcccgaa tggcagtgcg ccaaacagta cccacggacg
    41701 aaatttaccc cagcgagagc gcgttcgatc ggccaacaac cccatgcaag gatcggaaat
    41761 cgcatccagt gcacgagcga ccaaaaacat ggttccgaca aaaccggcag gaatgccaaa
    41821 aatatcggta taaaagaaca tcatatataa cattacgtta tcgaaaataa tgtggctggc
    41881 ggcgtctccc atgccataac caattttctc tttaacggac aacacttcac tcttcatctg
    41941 cttttccttg atgctatagg ggtgctgaga ccgataacgc attttgtaaa ccatccctgt
    42001 gagagcgaat attccgatat ctggttatta atttatgatt cttgttttat gtgatcgtgg
    42061 tagcgttaat ttcgctcata tatcattgta aaatataggt tttatatgaa ctataatgct
    42121 ttcgtgataa tacgctgcgt gtattaggcg gaaaaaactg atctggggga tgtagaaact
    42181 caaggaagta gctataatgc gccccgcctc catgtagcaa tcgaggcgcg gaagatcgtc
    42241 gtctccggtg aggcggctgg acttcaaatc cagttggggc cgccagcggt cccgggcagg
    42301 ttcgactcct gtgatcttcc gccagtttac ctcttctgaa ctctcccaat gtcaataaat
    42361 ctcttctaat cccttgtata tcagtattta tttgtatcca gaggtcaacc tacttcaccc
    42421 cgtatcaagt ttcttttggg ggtactttag ggggtgttat ttcctattga aataccagca
    42481 cccccataat atgaaaaatg tgatatataa caacggcttt taacaacatc tttgttatac
    42541 aaggggtgta ttgggggtat caggttagtt actggcagaa atccccctca ggaacatgaa
    42601 ttcatcaaaa gccggaggat gtcatggcat taaccgatat caaagtgaaa accgcaaaac
    42661 ccaaagacaa accctataag cttgctgatg gcggcggtat gtatctactg attaacacta
    42721 atggttctaa atactggcgc atgaagtacc gctttgctgg taaagaaaag atgctttcta
    42781 ttggcgtata ccccgatgta acgctagccg atgctcgcga gaaacgcagt gaagccagga
    42841 aactcctcgc tgctggaggc gatccagggg aggcaaaaaa ggaagaaaaa atcgcgcagc
    42901 aaatgagtct gaaaaatacg ttcgaggcta ttgctcgcga atggcaccag tcaaaagccg
    42961 atcggtggtc attacgctat cgtgatgaaa tcattgatac ctttgaaaaa gacatttttc
    43021 cctacattgg caagcgccct attgccgaaa tcaaaccaat ggagttactg gaagcactgc
    43081 gtaaaatgga aaaacgtgga gctctggaga aaatgcgcaa ggtccgccag cgctgtggtg
    43141 aggtgttccg ctatgcaatt gttactggcc gggcagacta taacccagcc cccgatcttg
    43201 ccagtgcttt agctacaccg aaaaaagtac attttccctt tcttactgct aatgaacttc
    43261 cccacttcct caacgatctg gcgggttata ccggaagtat aatcactaag acagctactc
    43321 agatcattat gttaaccggc gtacgaacac aagaactgcg ttttgcacgc tgggaagata
    43381 tcgattttga gacaaaattg tgggagatcc cggcagaagt aatgaagatg aagcgacctc
    43441 atattgtgcc gctctctgag caggtcatta tgctatttaa gcagcttgaa ccaatctcaa
    43501 aacatcatcc cctagtcttt atcggtagaa atgatccccg caaaccaatt agtaaggaaa
    43561 gcattaacca agtaattgaa ttacttggat ataagggaag actcacaggg cacggtttca
    43621 gacacaccat gagtacaatt ctgcatgaac aaggctttaa ttccgcctgg attgaaatgc
    43681 agttagctca tgtggataaa aattccatca ggggtaccta taatcatgcc ctgtatctcg
    43741 atggccgccg tgaaatgatg caatggtacg ctgattacat tgattcactc tccagccggg
    43801 aaagttaagt cagaaatact tttccctgat gttttcctca attaagtatt ttatcctc
//
LOCUS       DAZGXZ010000023        42691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000023 DAZGXZ010000000
VERSION     DAZGXZ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.023"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..581
                     /gene="yjdM"
                     /locus_tag="ACHKJF_001436"
     CDS             246..581
                     /gene="yjdM"
                     /locus_tag="ACHKJF_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP0948818.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            741..1184
                     /gene="yjdN"
                     /locus_tag="ACHKJF_001437"
     CDS             741..1184
                     /gene="yjdN"
                     /locus_tag="ACHKJF_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP0948819.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1317..2105
                     /gene="phnC"
                     /locus_tag="ACHKJF_001438"
     CDS             1317..2105
                     /gene="phnC"
                     /locus_tag="ACHKJF_001438"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP0948820.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2130..3146
                     /gene="phnD"
                     /locus_tag="ACHKJF_001439"
     CDS             2130..3146
                     /gene="phnD"
                     /locus_tag="ACHKJF_001439"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0948821.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3207..3986
                     /gene="phnE"
                     /locus_tag="ACHKJF_001440"
     CDS             3207..3986
                     /gene="phnE"
                     /locus_tag="ACHKJF_001440"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP0948822.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4007..4732
                     /gene="phnF"
                     /locus_tag="ACHKJF_001441"
     CDS             4007..4732
                     /gene="phnF"
                     /locus_tag="ACHKJF_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP0948823.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4733..5185
                     /gene="phnG"
                     /locus_tag="ACHKJF_001442"
     CDS             4733..5185
                     /gene="phnG"
                     /locus_tag="ACHKJF_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP0948824.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5182..5766
                     /gene="phnH"
                     /locus_tag="ACHKJF_001443"
     CDS             5182..5766
                     /gene="phnH"
                     /locus_tag="ACHKJF_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP0948825.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILYE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5766..6830
                     /gene="phnI"
                     /locus_tag="ACHKJF_001444"
     CDS             5766..6830
                     /gene="phnI"
                     /locus_tag="ACHKJF_001444"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP0948826.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTAHSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6823..7668
                     /gene="phnJ"
                     /locus_tag="ACHKJF_001445"
     CDS             6823..7668
                     /gene="phnJ"
                     /locus_tag="ACHKJF_001445"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP0948827.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7665..8423
                     /gene="phnK"
                     /locus_tag="ACHKJF_001446"
     CDS             7665..8423
                     /gene="phnK"
                     /locus_tag="ACHKJF_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP0948828.1"
                     /translation="MNKPLLSVNNLTHLYAPAKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(8378..8653)
                     /locus_tag="ACHKJF_001447"
     CDS             complement(8378..8653)
                     /locus_tag="ACHKJF_001447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948829.1"
                     /translation="MEHHAFARVDGERGAVEDGQAHAVLLVQDEGFTDVLYVNHFSPR
                     GSGVGCDADASYPTYGERICRPDKAFTPHPAPAQFCKTDDTSSCVYG"
     gene            8534..9214
                     /gene="phnL"
                     /locus_tag="ACHKJF_001448"
     CDS             8534..9214
                     /gene="phnL"
                     /locus_tag="ACHKJF_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP0948830.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            9211..10347
                     /gene="phnM"
                     /locus_tag="ACHKJF_001449"
     CDS             9211..10347
                     /gene="phnM"
                     /locus_tag="ACHKJF_001449"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP0948831.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10347..10904
                     /gene="phnN"
                     /locus_tag="ACHKJF_001450"
     CDS             10347..10904
                     /gene="phnN"
                     /locus_tag="ACHKJF_001450"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP0948832.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10891..11325
                     /gene="phnO"
                     /locus_tag="ACHKJF_001451"
     CDS             10891..11325
                     /gene="phnO"
                     /locus_tag="ACHKJF_001451"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP0948833.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11327..12085
                     /gene="phnP"
                     /locus_tag="ACHKJF_001452"
     CDS             11327..12085
                     /gene="phnP"
                     /locus_tag="ACHKJF_001452"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP0948834.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12232..12561)
                     /gene="yjdP"
                     /locus_tag="ACHKJF_001453"
     CDS             complement(12232..12561)
                     /gene="yjdP"
                     /locus_tag="ACHKJF_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP0948835.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12630..13079)
                     /gene="rpiB"
                     /locus_tag="ACHKJF_001454"
                     /gene_synonym="alsI"
     CDS             complement(12630..13079)
                     /gene="rpiB"
                     /locus_tag="ACHKJF_001454"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFP0948836.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQRRN"
     gene            13439..14329
                     /gene="alsR"
                     /locus_tag="ACHKJF_001455"
     CDS             13439..14329
                     /gene="alsR"
                     /locus_tag="ACHKJF_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFP0948837.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14388..15323
                     /gene="alsB"
                     /locus_tag="ACHKJF_001456"
     CDS             14388..15323
                     /gene="alsB"
                     /locus_tag="ACHKJF_001456"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFP0948838.1"
                     /translation="MNKYLKYFSSTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15450..16982
                     /gene="alsA"
                     /locus_tag="ACHKJF_001457"
     CDS             15450..16982
                     /gene="alsA"
                     /locus_tag="ACHKJF_001457"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFP0948839.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHETVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNMAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCCPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            16961..17941
                     /gene="alsC"
                     /locus_tag="ACHKJF_001458"
     CDS             16961..17941
                     /gene="alsC"
                     /locus_tag="ACHKJF_001458"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFP0948840.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17952..18647
                     /gene="alsE"
                     /locus_tag="ACHKJF_001459"
     CDS             17952..18647
                     /gene="alsE"
                     /locus_tag="ACHKJF_001459"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFP0948841.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18631..19560
                     /gene="alsK"
                     /locus_tag="ACHKJF_001460"
     CDS             18631..19560
                     /gene="alsK"
                     /locus_tag="ACHKJF_001460"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFP0948842.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPS
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
     gene            19833..21818
                     /gene="yjcS"
                     /locus_tag="ACHKJF_001461"
     CDS             19833..21818
                     /gene="yjcS"
                     /locus_tag="ACHKJF_001461"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP0948843.1"
                     /translation="MNNSRLFRLSRIIIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            22027..22302
                     /gene="ytcA"
                     /locus_tag="ACHKJF_001462"
     CDS             22027..22302
                     /gene="ytcA"
                     /locus_tag="ACHKJF_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP0948844.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
     gene            22321..23352
                     /gene="mdtN"
                     /locus_tag="ACHKJF_001463"
     CDS             22321..23352
                     /gene="mdtN"
                     /locus_tag="ACHKJF_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP0948845.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            23352..25403
                     /gene="mdtO"
                     /locus_tag="ACHKJF_001464"
     CDS             23352..25403
                     /gene="mdtO"
                     /locus_tag="ACHKJF_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP0948846.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNTCEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            25400..26866
                     /gene="mdtP"
                     /locus_tag="ACHKJF_001465"
     CDS             25400..26866
                     /gene="mdtP"
                     /locus_tag="ACHKJF_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP0948847.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            27064..29211
                     /gene="fdhF"
                     /locus_tag="ACHKJF_001466"
     CDS             27064..29211
                     /gene="fdhF"
                     /locus_tag="ACHKJF_001466"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:27481..27483,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFP0948848.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            29305..29994
                     /gene="yjcO"
                     /locus_tag="ACHKJF_001467"
     CDS             29305..29994
                     /gene="yjcO"
                     /locus_tag="ACHKJF_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP0948849.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(30072..31385)
                     /gene="gltP"
                     /locus_tag="ACHKJF_001468"
     CDS             complement(30072..31385)
                     /gene="gltP"
                     /locus_tag="ACHKJF_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP0948850.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(31727..32323)
                     /gene="nrfG"
                     /locus_tag="ACHKJF_001469"
     CDS             complement(31727..32323)
                     /gene="nrfG"
                     /locus_tag="ACHKJF_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP0948851.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(32320..32703)
                     /gene="nrfF"
                     /locus_tag="ACHKJF_001470"
     CDS             complement(32320..32703)
                     /gene="nrfF"
                     /locus_tag="ACHKJF_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP0948852.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(32696..34414)
                     /gene="nrfE"
                     /locus_tag="ACHKJF_001471"
     CDS             complement(32696..34414)
                     /gene="nrfE"
                     /locus_tag="ACHKJF_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP0948853.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(34434..35390)
                     /gene="nrfD"
                     /locus_tag="ACHKJF_001472"
     CDS             complement(34434..35390)
                     /gene="nrfD"
                     /locus_tag="ACHKJF_001472"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP0948854.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(35387..36058)
                     /gene="nrfC"
                     /locus_tag="ACHKJF_001473"
     CDS             complement(35387..36058)
                     /gene="nrfC"
                     /locus_tag="ACHKJF_001473"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP0948855.1"
                     /translation="MTWSRRQFLTGVGVLATVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(36055..36621)
                     /gene="nrfB"
                     /locus_tag="ACHKJF_001474"
     CDS             complement(36055..36621)
                     /gene="nrfB"
                     /locus_tag="ACHKJF_001474"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP0948856.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(36666..38102)
                     /gene="nrfA"
                     /locus_tag="ACHKJF_001475"
     CDS             complement(36666..38102)
                     /gene="nrfA"
                     /locus_tag="ACHKJF_001475"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP0948857.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            38495..40453
                     /gene="acs"
                     /locus_tag="ACHKJF_001476"
     CDS             38495..40453
                     /gene="acs"
                     /locus_tag="ACHKJF_001476"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP0948858.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            40653..40967
                     /gene="yjcH"
                     /locus_tag="ACHKJF_001477"
     CDS             40653..40967
                     /gene="yjcH"
                     /locus_tag="ACHKJF_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP0948859.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            40964..42613
                     /gene="actP"
                     /locus_tag="ACHKJF_001478"
     CDS             40964..42613
                     /gene="actP"
                     /locus_tag="ACHKJF_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP0948860.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
BASE COUNT        10135 a        11771 c        11348 g         9437 t
ORIGIN      
        1 gtcagaaaga taaaaatatc atgtgaataa aaaaaagaac aagtagagca ttaacattat
       61 cttaaataat aaatagaggc aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa
      121 acgatcgtcg tctaaaatca gcagtacccc cgacaaactc agggattttg tgtataattg
      181 cggccttttt cggcaatctg ccgttttttg gcgcttttgc cctgctgact tttgaggaaa
      241 tccacatgtc attaccacac tgcccaaaat gcaactccga atacacttac gaagataacg
      301 gcatgtacat ctgcccggaa tgtgcctacg aatggaacga cgcagaacct gcacaggaaa
      361 gcgacgagct gatcgttaaa gatgctaacg gcaatctgct ggctgacggc gacagcgtta
      421 ccatcattaa agatctgaag gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag
      481 tgaaaaacat ccgcctggtt gaaggcgacc ataacatcga ttgcaaaatc gacggttttg
      541 gtccgatgaa actgaaatct gagtttgtga aaaagaactg attgtattgt gatcggtaag
      601 ccggataagg cgctcgcgcc gcatccggca acgatgccga ctgcctgatg cgacgctgac
      661 cgcgtcttat caggcttccc tctcatctgt ataaatttcg aactacactt aactggcttc
      721 tcttaactga ggtcaccatc atgccgttaa gtccctacct ctcttttgcc ggtaactgtt
      781 ccgacgcgat tgcctattat caacgtacgt tgggcgctga actactctat aaaatcagct
      841 tcggcgaaat gccaaaatca gcgcaggaca gcgccgagaa ctgcccttcc ggaatgcaat
      901 ttcccgatac cgccatcgct catgccaacg tgcgcattgc cggaagcgac atcatgatga
      961 gcgatgccat gccgtcagga aaagccagct actccggctt tacgctggtg ctcgattcgc
     1021 aacaggtcga agaaggaaaa cgctggtttg acaatcttgc cgctaacgga aaaatcgaaa
     1081 tggcctggca ggaaactttc tgggcgcatg gctttggcaa agtcaccgat aaatttggcg
     1141 taccgtggat gattaatgtc gtcaaacaac aaccaacgca ataatccgcc gggaggcccg
     1201 ccctcccgca ctgtcatcga attcccgtta actcttcatc tgttagtcac ttttaattaa
     1261 ccaaatcgtc acaataatcc gccacgatgg agccactttt ttagggaggc tgcatcatgc
     1321 aaacgattat ccgtgtcgag aagctcgcca aaaccttcaa tcagcatcag gcgctgcatg
     1381 cggttgatct gaacattcat cacggtgaaa tggtggctct gcttgggccg tcgggttccg
     1441 gcaaatccac ccttttacgt cacttaagcg gtttgattac cggcgataaa tccgccggca
     1501 gccatatcga gctgctgggc cgcacagtcc agcgcgaagg ccgtctggcg cgcgatatcc
     1561 gcaaaagccg cgccaacacc ggctacatct tccaacaatt caacctggtg aaccgcctga
     1621 gcgtactgga gaacgtgctg attggcgcgc tcggcagcac gccgttctgg cgcacctgtt
     1681 ttagctggtt caccggcgag cagaaacagc gcgcgttaca ggcgctgacc cgcgttggca
     1741 tggtgcattt tgcccatcag cgcgtttcca ccctctccgg cggacagcag cagcgtgtgg
     1801 cgattgcccg cgcgctgatg cagcaggcga aggtgattct ggccgatgaa cccatcgcct
     1861 cgctggaccc ggaatccgcc cgcatcgtga tggacaccct gcgcgacatc aatcagaacg
     1921 acggcatcac cgtggtcgtc acgctgcatc aggtggatta cgccctgcgc tactgcgaac
     1981 gcatcgtcgc cctgcgccag gggcacgttt tctacgacgg cagcagccaa cagtttgata
     2041 acgaacgttt tgaccatctc taccgcagca ttaatcgcat cgaagagaac gcgaaagctg
     2101 cctgacatcc ccatcattga ggaaaacgaa tgaacgctaa gataattgcc tcgctggcct
     2161 tcaccagcat gttcagcctc agcaccctgt taagcccggc acacgccgaa gagcaggaaa
     2221 aggcgctgaa tttcggcatt atttcaacgg aatcacagca aaacctgaaa ccgcaatgga
     2281 cgccattctt acaggatatg gagaagaagc tgggcgtgaa ggtgaacgcc ttctttgccc
     2341 cagactacgc aggcattatc cagggaatgc gcttcaataa agtggatatc gcctggtacg
     2401 gcaacctgtc ggcaatggaa gcggtggatc gcgccaacgg ccaggtcttt gcccagacgg
     2461 tcgcggcgga tggatcgccg ggttactgga gcgtgttgat cgtcaacaaa gacagcccga
     2521 tcaacaacct gaacgatctg ctggcgaagc ggaaagatct caccttcggc aatggcgatc
     2581 ctaactccac ctctggcttc ctcgtccccg gttactacgt ctttgccaaa aacaatatct
     2641 ccgccagcga ctttaagcgc accgtcaacg ctgggcatga aactaacgcg ctggccgtcg
     2701 ccaacaagca ggtggatgtt gccaccaaca acaccgaaaa cctcgacaag ctgaaaacct
     2761 ccgcgccaga gaagctgaaa gaactgaagg tgatctggaa gtcgccgctg atcccaggcg
     2821 atccgatcgt ctggcgcaag aatctctccg aaaccaccaa agacaagatc tacgacttct
     2881 ttatgaacta cggaaaaacg ccggaagaga aagcggtgct ggaacgcctg ggctgggccc
     2941 cgttccgcgc ctccagcgac ctgcaactgg tgccgattcg ccagctcgca ctgtttaaag
     3001 agatgcaggg cgtaaaaagc aataaaggac tgaatgagca ggacaagctg gcgaaaacca
     3061 ccgagattca ggcgcagctg gatgacctgg accgcctgaa caacgcgtta agcgcgatga
     3121 gttcggtgag taaagcggtg cagtaatgtg ctcgatcggt cccctctccc acagggagag
     3181 ggagaaaaca ttaaacggag ccaaacatgc aaaccatcac catcgcccca cccaagcgta
     3241 gctggttctc gcttctgagc tgggccgttg ttctcgccgt gctggtcgtc tcgtggcagg
     3301 gcgcggaaat ggccccgctt acgctgatta aagacggcgg caacatggca accttcgctg
     3361 ccgacttctt cccgcccgat ttcagccagt ggcaggatta cctcaccgaa atggccgtca
     3421 cgctgcaaat cgccgtctgg ggcaccgcgc tggcggtggt tctctccatc ccctttggcc
     3481 tgatgagcgc cgaaaacctg gtgccgtggt gggtttacca gcccgttcgc cgcctgatgg
     3541 acgcctgccg cgccattaac gaaatggtct tcgccatgct gttcgtggtc gccgtcggtc
     3601 tcggaccgtt cgctggcgtg ctggcgctat ttatccacac caccggcgtg ctctccaagc
     3661 tgctttccga ggcggtggaa gcgattgaac ccggcccggt ggaaggcatt cgcgccaccg
     3721 gtgccaacaa gctcgaagag atcctctacg gcgtgctgcc acaggtgatg ccgctgctga
     3781 tctcctactc cctctatcgc ttcgaatcca acgtccgctc ggcgaccgtc gtcggcatgg
     3841 tcggcgcagg cgggatcggc gtcaccctgt gggaagcgat tcgcggtttc cagttccagc
     3901 aaacctgcgc cctgatggtg cttatcatcg tcacggtcag cctgctggat ttcctctctc
     3961 aacggttgcg taagcacttt atctgataag cgaggcattg atatctatgc acttgtctac
     4021 acatccgacc agctacccaa cacgctatca ggagatagcc gcaaaacttg agcaggagct
     4081 tcgtcaacac tatcgctgcg gcgactatct tcccgccgag cagcagctgg cggcgcgctt
     4141 tgaggtaaat cgccacaccc tgcgccgcgc catcgaccaa ctggtggaaa aaggctgggt
     4201 acagcgccgt cagggcgtcg gcgtgctggt gctgatgcgc ccgttcgatt acccgctcaa
     4261 cgcccaggcg cgttttagcc agaacctgct ggatcagggc agccatccca ccagcgaaaa
     4321 actgctgtcg gtgctgcgcc cggcatctgg tcacgtcgct gacgcgctgg ggataaccga
     4381 gggggagaac gtcatccacc tgcgcaccct gcgccgggtc aacggcatcg cgctctgttt
     4441 aatcgaccac tactttgcgg acctcaccct ctggccgaca ctgcaacgtt tcgacagcgg
     4501 ttcgctgcac gattttctgc gcgagcaaac cggaattgcg ctgcgccgca gccagacgcg
     4561 gatcagcgcc cgccgcgccc aggccaaaga gtgccagcgc cttgaaatcc cgaatatgtc
     4621 gccgctgctg tgcgtgcgca cccttaacca ccgtgacggt gaaagcagcc cggcggagta
     4681 ctccgtcagc ctgacgcgcg ccgacatgat tgaattcact atggagcact gaatgcacgc
     4741 agataccgcg acccgccagc actggatgtc cgtactggcg cacagccaac cggctgaact
     4801 ggcagcccgc ctgaacgcgc tgaacatcac cgccgactat gaagtgatcc gcactgccga
     4861 aactggcctg gtgcagattc aggcgcgaat gggcggtacc ggcgaacgct tttttgccgg
     4921 cgacgccacg ctgacccgcg ccgccgtacg cctgactgac ggcacgctcg gctacagctg
     4981 ggtgctgggg cgcgacaaac agcacgccga acgctgcgcg ctgattgacg cgctgatgca
     5041 gcaatctcgc cactttcaaa acttatcaga aactcttatt gccccgctgg acgctgaccg
     5101 tatggcacgc attgccgcac gccaggccga agtgaacgcc agccgggtcg acttctttac
     5161 gatggttcgc ggagataacg catgaccctg gaaaccgctt ttattcttcc tgtgcaggat
     5221 gcccagcaca gttttcgtcg cctgttaaag gccatgagcg agccgggcgt gattgtcgcc
     5281 ctgcatcagc tcaaacgcgg ctggcagccg ctgaatatcg ccaccaccag cgtgctgctg
     5341 acgctggccg ataacgacac gccggtgtgg ctttccgcgc cattgagtaa cgacatcgtc
     5401 agccagagcc tgcgttttca taccaacgcg ccgctggtca gccagccgga acaggcgacc
     5461 ttcgcggtaa cggatgaggc gatttccagc gaacagctta acgccctgtc caccggcacc
     5521 gccgttgcgc cggaagcggg tgcaacgctg attttacagg tcgccagcct gagcggcgga
     5581 cgcatgttac gtctcaccgg cgcgggcatt gccgaagaac gaatgatcgc cccgcagctg
     5641 ccggagtgca ttctgtacga actcaccgag cgcccgcacc cgttcccgct cggcatcgac
     5701 ctgatcctga cctgcggcga gcgcctgctg gctattccgc gaaccacgca tgtggaggtg
     5761 tgctgatgta cgttgccgtg aaagggggcg aaaaggcgat cgatgccgcc cacgccctgc
     5821 aagagagccg acgccggggc gataccgatt tgcctgaact gagcgtcgcc cagattgaac
     5881 agcagcttaa cctcgcagta gatcgcgtga tgaccgaagg cggcattgcc gaccgcgaac
     5941 tggcggcgct ggcgctgaaa caggccagcg gcgataacgt cgaagcaatt ttcctgctgc
     6001 gcgcctaccg caccacgctg gcgaagctcg cggtaagcga gccgctcgac accacggaga
     6061 tgcgtctcga acggcgtatt tcagcggttt ataaagatat tcccggcggc cagttgcttg
     6121 gcccaaccta cgactacacc catcgcctgc tcgatttcac cctgctggca aacggcgaag
     6181 cgccgacgct gaccaccgcc cacagcgaac aacagccgtc gccgcacgtt ttcagcctgc
     6241 tggcgcgtca ggggctggcg aagtttgaag aggatagcgg cgcacagccg gatgacatca
     6301 cccgcacgcc gccggtttac ccctgctcac gctcctcccg tttacagcag ttgatgcgcg
     6361 gcgacgaagg ctatttgctg gcgctggcct actccaccca acgcggctac ggtcgcaacc
     6421 acccgttcgc aggcgagatc cgcagcggct atatcgacgt atcgattgtg ccggaagagc
     6481 tgggatttgc ggtaaacgtc ggcgaactac tgatgaccga gtgtgaaatg gtcaacggtt
     6541 ttatcgaccc gccggatgag ccgccgcact tcacgcgcgg ctacgggctg gtattcggca
     6601 tgagcgagcg caaagcgatg gcaatggcgc tggtcgatcg tgcgttgcag gctccggaat
     6661 acggcgagca cgcgacaggc ccggcgcagg atgaagagtt tgtgctggca catgccgaca
     6721 acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc ccactacgtc gatttccagg
     6781 ccgaactgga gctactcaaa cgtctgcaac aggagaagaa ccatggctaa tctgagcggc
     6841 tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc tatcttaaaa
     6901 gcggtggcga tccccggtta tcaggtgccg tttggcgggc gcgagatgcc gatgccatac
     6961 ggctggggaa ccggcggcat acagctcacc gccagcgtga ttggcgaaag cgacgtgctg
     7021 aaggtgattg accagggtgc ggatgacacc accaacgccg tgtcgattcg caacttcttt
     7081 aagcgcgtga ccggggtaaa caccactgaa cgtacggacg atgcgacgct tatccagacg
     7141 cgtcaccgca tccccgaaac gccgctgacc gaagatcaga tcattatctt ccaggtgcca
     7201 atccccgagc cgctgcgctt tatcgagccg cgcgaaacgg aaacccgcac catgcacgcg
     7261 ctggaggagt acggcgtgat gcaggtgaaa ctgtatgaag atattgcccg cttcggtcat
     7321 atcgccacta cctacgccta tccggtgaag gttaacgggc gctacgtaat ggacccgtcg
     7381 ccgatcccga aattcgataa cccgaaaatg gacatgatgc ccgccctgca actgttcggc
     7441 gcggggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga aagtctcgat
     7501 ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat ctgcggatcg
     7561 acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat gtttgtctgc
     7621 tccgataccg attattgccg ccaacagagc gaggcgaaaa gccaatgaat aaaccgttac
     7681 tttcggtcaa taacctgacc cacctttacg cgccggccaa aggctttagc gatgtctctt
     7741 ttgatttatg gccgggagaa gtgctgggca ttgtcggaga atccggctcc gggaagacca
     7801 cgctgctgaa gtcgatctcc gcgcgtctga cgccgcagca gggggagatt cactacgaga
     7861 accgttcgct gtatgcaatg agcgaggccg accgccgtcg cctgctgcgc accgaatggg
     7921 gcgtagtgca tcagcatcca ctcgacggcc tgcgccgtca ggtctcggca ggcggcaata
     7981 tcggcgagcg gctgatggcg accggggcgc gtcattacgg cgacattcgc gccaccgcgc
     8041 agaagtggct ggaagaggtg gaaattcccg ccaaccggat cgacgacctg ccgaccacct
     8101 tttccggcgg catgcagcaa cgtttgcaga ttgcccgcaa cctggtgacg catccgaagc
     8161 tggtgtttat ggatgaaccg accggcgggc tggatgtgtc ggtgcaggcc cgcctgctcg
     8221 acctgctgcg cggcctggtg gtggagctga acctggcggt ggtgattgtc acccatgatt
     8281 taggcgttgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg caagtggtgg
     8341 agagtgggtt aaccgaccgc gtgctcgacg acccgcatca tccgtataca cagctgctgg
     8401 tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc ttatccggcc
     8461 tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg acacccgaac
     8521 cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc tgcaccagca
     8581 aaacggcgtg cgcctgcccg tcctcaaccg cgcctcgctc accgtcaacg cgggcgaatg
     8641 cgtggtgctc cacggtcact ccggcagcgg caaatcaacc ctgctgcgct cgctgtacgc
     8701 caactatctg cccgatgaag gtcaaatcca gatcaaacac ggtgacgagt gggtagacct
     8761 ggtcaccgcg ccagcccgca aagtggtgga gatccgcaaa accaccgtcg gctgggtgag
     8821 ccagtttctg cgcgtcatcc cgcgtatttc agcactggaa gtggtgatgc agccgctgct
     8881 cgataccggc gttccgcgtg aagcctgcgc cgctaaagcc gcgcgtctgc tcacccgcct
     8941 gaacgtgccg gaacgcctgt ggcacctggc gccatcaacc ttttccggtg gcgaacagca
     9001 gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc tcgacgaacc
     9061 taccgcctcg ctggacgcca aaaacagcgc cgccgtagtc gagctgattc gcgaagccaa
     9121 aacccgtggc gcggccatcg tcggcatctt ccatgacgaa gccgtacgta atgacgtcgc
     9181 cgaccgcttg cacccaatgg gagcctctgc atgattatca ataacgtcaa actggtgctg
     9241 gaaaacgaag tggtacacgg ttcgctggag gtgcaggatg gcgaaatccg cgcctttgcc
     9301 gaaagccaga gccgcctgcc agaagcgatg gacggcgaag gtggctggct actacccggc
     9361 ctgattgagc tgcataccga caacctcgat aaattcttca ccccgcgccc gaaagtcgac
     9421 tggcccgccc attcggcgat gagcagccac gacgcgctga tggtggcaag cggcatcacc
     9481 accgtgctgg acgcggtggc gattggcgac gtgcgcgacg gcggcgatcg gctggagaat
     9541 ctggagaaga tgatcaacac catcgaagag acgcagaaac gcggcgtcaa ccgcgccgag
     9601 caccgtctgc acctgcgctg cgagctgccg catcacacca cactgccgct gtttgaaaaa
     9661 ctggttcagc gcgaaccggt gacgctggtg tcgctgatgg accactcacc gggccagcgc
     9721 cagttcgcta accgcgagaa gtatcgcgaa tattatcagg gcaaatactc cctcaccgat
     9781 gcgcagatgc agcagtacga agaagagcaa ttggcgctcg ccgcacgctg gtcgcagccg
     9841 aatcgcgaat ccatcgccgc cctgtgccgc gcgcgacaaa ttgcgctcgc cagccacgat
     9901 gacgccacgc acgcccacgt tgccgaatcc caccaacttg gcagcgtgat cgccgaattt
     9961 cccaccacgt tcgaagcggc ggaagcctcg cgtaagcatg gcatgaacgt gctgatgggt
    10021 gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg tggcggccag tgaactggcg
    10081 cagcttggtc tgctggatat cctctcttcc gactactacc cagccagcct gctggatgcg
    10141 gcgttccgcg tcgccgatga cgagagcaac cgctttacgc taccgcaggc ggtgaggctg
    10201 gtgaccaaaa atccggcgca ggcgctgaat cttcaggatc gcggggtgat tggcgagggt
    10261 aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc acattcatat cgaccacgtc
    10321 tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta atggggccgt
    10381 ccggctccgg gaaagacagc ctgctggcgg aactccgcct gcgggaacaa acgcagttac
    10441 tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagtgaaaac catatcgccc
    10501 tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct gctggcctta agctggcacg
    10561 ctaacggtct gtattatggc gtcggcatcg agattgacct ctggctgcac gctggattcg
    10621 acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc acgggcgcgc tatcaatcgg
    10681 cgctgctgcc cgtctgttta caggtttcgc cggagatcct gcgccagcgc ctggaaaacc
    10741 gtggccgtga aaacgccagt gaaattaacg cccgcctggc gcgcgccgcc cgctatactc
    10801 cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc gacacgctgc
    10861 tgacgctgat ccatcagaag gagaaacacc atgcctgctt gtgagcttcg cccggccacg
    10921 cagtacgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc ggagtttgac
    10981 caccatgcgt ttcgcgtggg ctttaacgcc aacctgcgcg acccaaacat gcgctaccat
    11041 ctggcgctgc tggatggcga agttgtcggc atgatcggcc tgcatttgca gtttcatctg
    11101 catcatgtca actggatcgg cgaaattcag gaactggtgg tgatgccgca ggcgcgcggt
    11161 ctgaacgtcg gcagtaaatt actggcatgg gcagaagaag aagcccgcca ggccggggcc
    11221 gaaatgaccg agctttccac caacgtgaag cgccacgacg cacaccgttt ttacctgcgt
    11281 gaaggctacg agcagagcca cttccgcttc accaaggcgc tgtaacatga gcctgaccct
    11341 cacgctcacc ggcactggcg gcgcacaggg cgttccggca tggggctgcg agtgcgcggc
    11401 ctgcgccaga gcgcggcgct cgccgcagta tcgccgccag ccatgcagcg gcgtagtgaa
    11461 gtttaacgac gcgatcaccc tgatcgacgc cgggcggcac gatctcaccg atcgctggtc
    11521 gcctggatcg ttccagcagt ttttgctgac gcactaccat atggatcacg tccaggggct
    11581 atttccgcta cgctggggcg tgggcgatgt gatcccggtc tacggcccgc cggatgaaca
    11641 gggctgcgac gatctgttta aacatccggg cctgcttgat ttcagccaca cagtggaacc
    11701 gtttgtggtg tttgatttgc aggggttgca ggtcacgccg ctgccgctta accactcaaa
    11761 actgaccttc ggttatctgc tggaaacggc acatagccgg gtggcgtggc tgtctgacac
    11821 cgctggcctg ccggaaaaaa cgctgaaatt tttactcaat aatcatccgc aggtgatggt
    11881 gattgattgc agtcacccgc cgcgcgcgga tgcaccgcgt aatcactgtg atttaaatac
    11941 cgtgcttgcg ctgaatcagg ttatccgctc gccacaggtg attctgaccc atatcagcca
    12001 ccagtttgat gcgtggctga tggaaaacgc actaccgtca gggtttgagg tggggtttga
    12061 tgggatggag attggggtgg cgtgatgaga gggaatgtgc gcgctggccc cctcacccta
    12121 accctctccc cagaggggcg aggggaccga ttgtgctcaa tattgaatat tgcgctcgtt
    12181 ttctccctct ccccattggg gtgaggggcg atgcctgctc catacccaac ctcatcgccc
    12241 atactcatct tccattctcc gctcttcatc ctccagttgc cgacgctcct gatcaagctg
    12301 gcgctggcga tcgtccagct gcctgcggcg atcttcaaac tggcggcggc ggtcgtcata
    12361 ttgtctgcgc cgatcgtcgc tcacttcacg ctgccagccg tcgtcgcgcg aatcttcata
    12421 gtctcgccca cggtcagggt tataagcgtc attaatcgcc tgctgaatat taccaatagt
    12481 atcgtcgata atatcagcct gggccggaac ggtggacagc gtgagcaggg tgaataaaag
    12541 aaatagcgga aagcgtttca ttagccaacc tcaaaaagaa actctatcca cattagtcat
    12601 tactcatcca tgcaagtagt ggatgaatct caatttctcc gctgctctat tgccgtaatc
    12661 gcctccacgc gttgttgatg acgaccgcct tcgtactgtg cgcccagcca cgcatccaca
    12721 atcatttttg ccagttcgag gccaaccact cgtgaaccaa aagccagcac gttggtgtcg
    12781 ttatgctgcc gcgaaagttg cgcggaataa ggttcgctac agacgaccgc gcgaattccg
    12841 gcaaacttgt tcgccgctat cgaaataccg acgccagtac cacaaatcaa aatcccgcca
    12901 tcaacctctc cgccagcaac agccagtgcg acttgactgg cgtaatgcgg ataatcagta
    12961 cgctctgacg accaggttcc tttatcaatc acttcaacgc cacgctcaac taaatgtgcc
    13021 actatttcat gttttaaaat gaaaccgaca tgatcacagc caaatgcaat ctttttcatt
    13081 catcttcccc gttcgtgcaa aaacttcaca atcaaaaaac atttgtgatg taaatgaatt
    13141 aaaaacaccc aaaatcgatg aaatccgcct tcctgatctg ttttacagag ttacatctga
    13201 cagcgcaata gcgttaaaga cacttactat ccagcaacat ctatcatcta aaaaaccaga
    13261 aaaacaaata acatcatgtt tttaaagtga ttgaatgaaa taaaatttta agccactatc
    13321 catttttcac aacaaaacaa actttataaa ttttattttt ttgtgaagta gccagcatct
    13381 tttctgttct tgctgtggtg atatagtggc gtcttcaatt caaggacaag agaacgtgat
    13441 gagccagtca gagtttgatt cagcgcttcc gaacggtata gggttagcgc cttacctgcg
    13501 aatgaaacag gaaggaatga cagaaaatga aagccgcatc gtggagtggt tactcaaacc
    13561 cggtaacctg agttgtgcac ccgcaattaa agatgtcgca gaagctctgg cggtatctga
    13621 agcgatgata gttaaggtat caaagctgct ggggtttagc ggctttcgta acttacgcag
    13681 tgcgctggaa gattattttt ctcagtcaga acaagtattg ccttccgagt tggcttttga
    13741 tgaagcgccg caggatgtgg tgaataaggt atttaacatc actttacgca ccattatgga
    13801 aggtcagtcg atcgtcaacg ttgatgagat ccaccgtgcc gcccgctttt tctatcaggc
    13861 cagacagcgg gatttatacg gtgccggagg atcaaatgct atctgtgctg atgtacagca
    13921 caagttcttg cgcattggcg tacgctgtca ggcctatccg gacgctcaca tcatgatgat
    13981 gtccgcttcg ttgttacagg aaggagatgt tgtgctggta gtgacccatt ccgggcgaac
    14041 cagtgatgta aaagcggccg tagaactggc aaaaaagaac ggggcaaaga ttatttgtat
    14101 aacgcatagc taccattcac caatagcgaa actggccgat tatattattt gctcaccagc
    14161 cccggaaacg ccgttattag gtcgtaatgc ctcggcaaga atattacaac taactttgct
    14221 ggacgctttt tttgtctctg tcgcccagct caacattgaa caagctaata ttaatatgca
    14281 aaaaaccggc gcaattgttg atttcttctc accaggcgcg ctgaaataag aaaaaggtaa
    14341 ttccgccatc gcggagttac ctgtcccctt accgtgagaa caatattatg aataaatatc
    14401 tgaaatattt cagcagcaca ctcgtgggct taatgttgtc aaccagcgct tttgctgccg
    14461 ccgaatatgc tgtcgtattg aaaaccctct ccaacccatt ttgggtagat atgaaaaaag
    14521 gcattgaaga tgaagcaaaa acactgggcg tcagcgttga tatttttgcc tctccttcag
    14581 aaggcgattt tcaatctcaa ttgcagttat ttgaagatct cagtaataaa aattacaaag
    14641 gtatcgcctt cgctccatta tcctcagtga atctggtcat gcctgtcgcc cgcgcatgga
    14701 aaaaaggcat ttatctggtt aatctcgatg aaaaaatcga catggataat ctgaaaaaag
    14761 ctggcggcaa tgtggaaggt tttgtcacca ccgataacgt tgctgtcggg gcgaaaggcg
    14821 cgtcgttcat tattgacaaa ttgggtgctg aaggtggtga agtcgcaatc attgagggta
    14881 aagccggtaa cgcctccggt gaagcgcgtc gtaatggtgc caccgaagcc ttcaaaaaag
    14941 caagccagat caagcttgtc gccagccagc ctgccgactg ggaccgcatt aaagcactgg
    15001 atgtcgccac taacgtgttg caacgtaatc cgaatattaa agcgatctat tgcgcgaatg
    15061 acacgatggc aatgggtgtt gctcaggcag tcgcaaacgc cggaaaaacg ggcaaaatat
    15121 tggtcgtcgg tacagatgga attccggaag cccgcaaaat ggtggaagcc ggacaaatga
    15181 ccgcgacggt tgcccagaac ccggcagata ttggcgcaac gggtctgaag ctgatggttg
    15241 acgctgagaa atccggcaag gttattccgc tggataaagc accggaattt aaactggtcg
    15301 attcaatcct ggtcactcaa taaaactgaa tgatgccgga tgatgatgct aatgaagtgt
    15361 cttatccggc caacaaaata acctgaacgt agacctgata aacgtagcca tcaggcaata
    15421 atacgatatt tcgtgcagag aggttattta tggccacgcc atatatatcg atggcgggga
    15481 tcggcaagtc ctttggtccg gttcacgcat taaagtcggt taatttaacg gtttatcctg
    15541 gtgaaataca tgcattacta ggagaaaatg gcgcgggtaa atccacgcta atgaaggttt
    15601 tatccggaat acatgagccg accaaaggca ccattactat taataacatt aactataaca
    15661 agctggatca taaattagcg gcacaactcg gtatcgggat tatttatcag gaactcagcg
    15721 ttattgatga attaaccgta ctggaaaatt tatatattgg tcgtcatctg acgaaaaaaa
    15781 tctgtggcgt caatattatc gactggcgag aaatgcgtgt ccgcgccgcc atgatgttat
    15841 tacgcgtggg cttgaaagtt gatttagatg agaaagtggc gaatttatct atcagccaca
    15901 agcagatgct ggaaattgcc aaaacgctga tgctcgatgc caaagtcatc atcatggatg
    15961 aacccacctc ctcactcacc aataaagagg tggactatct gtttctgatc atgaatcagt
    16021 tgcgtaaaga gggtacggcc atcgtctata tctcgcataa gttggcggaa attcgccgta
    16081 tttgcgaccg ctatacggtg atgaaagacg gcagcagcgt ttgcagcggc atggtgagcg
    16141 atgtgtcaaa tgacgatatc gtccgtctga tggtaggccg cgaattgcaa aaccgtttta
    16201 acgcgatgaa ggagaatgtc agcaaccttg cgcacgaaac ggtttttgag gtgcgcaacg
    16261 tcaccagccg tgacagaaaa aaggtccggg atatctcatt tagcgtctgc cggggagaaa
    16321 tattaggctt tgccgggctg gtcggttccg gacgtactga actgatgaat tgcctgtttg
    16381 gcgttgataa acgcgctggc ggagaaatcc gtcttaatgg caaagatatc tctccacgtt
    16441 cccccctgga tgccgtgaaa aaagggatgg cttacatcac tgaaagccgc cgggataacg
    16501 gttttttccc caacttttcc atcgctcaga acatggcgat cagccgcagt ctgaaagacg
    16561 gcggctataa aggcgcgatg ggcttgtttc atgaagttga cgagcaacgt accgctgaaa
    16621 atcaacgcga actgctggcg ctgaaatgtc attcggtaaa ccagaatatc accgaactct
    16681 ccgggggaaa tcagcagaaa gtcctgatct ccaaatggct gtgctgttgc ccggaagtga
    16741 ttattttcga tgaacctacc cgcggcatcg acgttggcgc gaaagccgaa atttacaaag
    16801 tgatgcgcca actggcggac gacggaaaag tcatcctgat ggtgtcatct gaactacctg
    16861 aaattatcac cgtctgcgac cgcatcgccg tgttctgcga aggacgactg acgcaaatcc
    16921 tgacgaatcg cgatgacatg agcgaagagg agattatggc atgggcttta ccacaagagt
    16981 aaaaagcgaa gcgagcgaga agaaaccgtt caactttgcg ctgttctggg ataaatacgg
    17041 cacctttttt atcctggcga tcatcgtcgc catttttggt tcgctgtcgc cagaatattt
    17101 tctgaccacc aataatatta cccagatttt tgttcaaagc tccgtgacgg tattgatcgg
    17161 catgggcgag tttttcgcta tcctggtcgc tggtatcgac ctctcggttg gcgcgattct
    17221 ggcgctttcc ggtatggtga ccgccaaact gatgttggca ggtgttgacc cgtttctcgc
    17281 agcgatgatt ggcggtgtac tggttggcgg cgcactgggg gcgatcaacg gctgcctggt
    17341 caactggacg gggctacacc cgttcatcat cacccttggc accaacgcga ttttccgtgg
    17401 gatcacgctg gtgatctccg atgccaactc ggtatacggc ttctcctttg acttcgtgaa
    17461 cttctttgcc gccagcgtaa ttgggatacc tgtccccgtt atcttctcgc taattgtcgc
    17521 gctcatcctt tggtttctga caacgcgtat gcggctcggg cgcaacatct acgcactggg
    17581 cggcaacaaa aattcggcgt tctattccgg gattgacgtg aaattccaca tcctggtggt
    17641 gtttatcatc tccggtgttt gtgcaggtct ggcaggcgtc gtctcaactg cacgactcgg
    17701 tgccgcagaa ccgcttgccg gtatgggttt tgaaacctat gccattgcca gcgccatcat
    17761 tggcggcacc agtttcttcg gcggcaaggg gcgcattttc tctgtggtga ttggcgggtt
    17821 gatcatcggc accatcaaca acggtctgaa tattttgcag gtacaaacct attaccaact
    17881 ggtggtgatg ggcggattaa ttatcgcggc tgtcgccctt gaccgtctta tcagtaagta
    17941 aggaattgaa catgaaaatc tccccctcgt taatgtgtat ggatctgctg aaatttaaag
    18001 aacagatcga atttatcgac agccatgccg attacttcca catcgatatc atggacggtc
    18061 actttgtccc caatctgaca ctctcaccgt tcttcgtaag tcaggttaaa aaactggcaa
    18121 ctaaaccgct cgactgtcat ctgatggtga cgcggccgca ggattacatt gctcaactgg
    18181 cacgtgcggg agcagatttt atcactctgc atccggaaac catcaacggc caggcgttcc
    18241 gcctgattga tgaaatccgc cgtcatgaca tgaaagtggg gctgatcctt aatccggaga
    18301 cgccagttga ggccatgaaa tactatatcc ataaggccga taaaattacg gtcatgactg
    18361 tcgatcccgg ctttgccgga caaccgttca ttcctgaaat gctggataaa cttgccgaac
    18421 tgaaggcatg gcgtgaacga gaaggtctgg agtacgaaat tgaggtggac ggttcctgca
    18481 accaggcaac ttacgaaaaa ctaatggcgg caggagcgga tgtctttatc gtcggcactt
    18541 ccggcctgtt taatcatgcg gaaaatatcg acgaagcatg gagaattatg accgcgcaga
    18601 ttctggctgc aaaaagcgag gtacagcctc atgcaaaaac agcataacgt cgtagcgggc
    18661 gtggatatgg gggcaacgca tatccgcttt tgtctgcgga cagcagaagg tgaaacgcta
    18721 cactgcgaaa aaaagcggac cgcagaagtc attgctccca gcctggtgtc gggtatcggc
    18781 gaaatgattg acgagcaact caggcgcttt aacgctcgct gtcatggtct ggtgatggga
    18841 tttccggcgc tggtcagtaa agataaacgc accataattt ctacgcctaa cctgccgtta
    18901 acagcggcgg atttatatga tctcgccgat aagctcgaaa atacgctgaa ttgtccggtt
    18961 gagttttccc gcgacgttaa cctgcaactc tcctgggacg tagtagaaaa ccgccttacg
    19021 caacaactgg ttctggcggc ctatctcggt acggggatgg ggttcgcagt gtggatgaac
    19081 ggtgcgccgt ggacgggtgc acacggtgtg gcaggcgaac tgggtcatat ccccctggga
    19141 gatatgaccc aacactgcgc gtgtggcaat cctgggtgcc tggaaaccaa ttgctctgga
    19201 atggcgctaa gacgctggta cgaacaacag ccccgaaatt acccattgag cgaccttttc
    19261 gtccatgcgg aaaacgcccc tttcgtccag agtctgcttg aaaacgcggc acgggccatt
    19321 gccaccagca ttaatctgtt cgatcccgat gcggtgattc tgggcggtgg cgtgatggat
    19381 atgcccgcct tcccacgcga gactctcatt gccatgaccc aaaagtacct gcgccgtcca
    19441 ctgccgcatc aggtcgtgcg ctttattgcc gcctcatctt ctgactttaa tggcgctcag
    19501 ggtgcagcaa tattggcgca tcaacatttt ttgccacagt cctgtgctaa agccccatga
    19561 gtaaagcgtt ggcacagtag cgcaatgtcc cgctgtgcca acgacaggta caatatatat
    19621 aataaacaca taaaacggct catattattt tctgcaatga tagttttact gtaacttttc
    19681 ctcttcagca caaatgccta ataaaatgga tcgttcagct cacacttcca cttattaaag
    19741 tcgcaaattc ccaattatga taaccccagc tttaaatgac gtattttgca tggattacat
    19801 atttacgaca taaatgataa atggagattt atatgaataa ctctcggtta ttccgtttga
    19861 gcagaattat tattgcgtta actgccgcca gcggcatgat ggtaaatacc gctaacgcga
    19921 aagaggaagc gaaagccgcc actcaatata cccaacaggt taatcagaat tacgccaaat
    19981 cattaccgtt tagcgatcgt caggattttg acgacgctca gcgtggattt atcgctccgc
    20041 tgctggatga aggtattctg cgcgatgcta atggcaaacc atattatcgc ggggaagatt
    20101 ataaatttga tatcaatgcg cccgcgcctg aaaccgttaa ccctagcctg tggcgtcagt
    20161 cgcagttaaa cggtatttcc ggcctgttta aagtcaccga cagaatgtac caggttcgcg
    20221 gtcaggatat ctccaacatc acctttatcg aaggtgatac cgggattatt gtcatcgacc
    20281 cgctggtgac gccaccgagc gcaaaagccg cccttgacct ttacttccaa aatcgcccgc
    20341 aaaaaccgat tgttgcggtt atttataccc acagccatac cgaccactat ggcggcgtga
    20401 aaggcattat ctctgaagcc gatgtgaaat ccggtaaagt gcaggttatc gcccctgctg
    20461 gctttatgga cgaagccatc agcgaaaacg tactggcggg taatattatg agccgccgtg
    20521 cactttactc ctacggcctg ctgctggcgc ataaccctca gggtaacatc ggcaatggtc
    20581 ttggcgtaac gctggcatcg ggctacccga gcatcatcgc accgaacaaa accatcacca
    20641 aaaccggtga gaagatgatt atcgacggcc tggagtttga cttcctgatg accccaggta
    20701 gcgaagcacc agccgaaatg cacttctata ttccggccct gaaagcgctg tgtaccgccg
    20761 agaacgccac gcataccctg cacaacttct acactctgcg cggcgcgaaa acccgcgaca
    20821 ccagcaagtg gaccgagtat ctgaacgaaa cgctggatat gtggggtaac gacgcggaag
    20881 tcctgtttat gccgcacacc tggccggtct ggggcaataa gcatatcaat gattatattg
    20941 gtaaatatcg cgatactatc aagtacattc acgaccagac cctgcacctg gcgaaccagg
    21001 gctacaccat gaatgaaatc ggcgacatga ttaaactgcc gcctgcactt gccaataact
    21061 gggccagccg tggctattac ggttctgtca gccacaacgc ccgcgcggtg tataacttct
    21121 atcttggcta ttacgacggt aacccggcta acctgcatcc gtatggtcag gtggagatgg
    21181 gtaaacgtta cgtgcaggcg ctgggcggtt ctgcccgtgt catcaacctg gcgcaagaag
    21241 cgaacaagca aggtgattac cgctggtcgg cagaactgct gaaacaggtg attgccgcca
    21301 acccgggtga ccaggtcgcg aagaatctgc aagcgaataa ctttgaacag ctgggctatc
    21361 aggccgagtc cgccacctgg cgcggtttct acctgaccgg cgcgaaagag ctgcgcgaag
    21421 gggtgcataa gttcagccac ggcaccaccg gttccccgga caccattcgc gggatgtcgg
    21481 tcgaaatgct gttcgacttt atgtccgttc gcctcgatag cgcgaaagcc gcgggtaaaa
    21541 atatcagcct gaacttcaat atgagcaatg gcgataacct caacctgacg ctgaacgata
    21601 gcgtgcttaa ctaccgtaaa acactgcaat cccaagctga cgcctctttc tacatcagcc
    21661 gtgaagatct gcacgccgtg ctgaccgggc aagccaaaat ggcggatctg gtaaaagcga
    21721 agaaagccaa aattattggc aatggcgcga aactggaaga aattatcgcc tgtctggata
    21781 atttcgattt gtgggtgaat atcgtaaccc caaattaatg tcactcgctt ttgggggcgc
    21841 tgaagccccc aaaagcattt attttaaaaa tatatacagc caattaataa ataaaatata
    21901 taagttatga atgcggtggc tgaataaagc tatttttatt catcaagaaa atagccatac
    21961 tgacgccttc aatggatgtt gatattcact acagatattg ttcgcagcag acaggatgta
    22021 tttaatatgc caacagttct ctctcgcatg gcgatgcaac tcaaaaaaac agcctggata
    22081 attcccgtct tcatggtttc gggatgctca ttatctccgg caatcccggt gatcggcgct
    22141 tattatccca gctggttttt ctgcgccatt gccagcctta ttttgatgct catcacgagg
    22201 cgagttattc agcgggcaaa tatcaatctg gcatttgtcg gaattattta taccgccctt
    22261 tttgctctct acgccatgct gttctggctg gcatttttct aatctatttg agacgcgctc
    22321 atggaaagta cgccgaaaaa agctcctcgc agtaaattcc ctgctctgtt agtggttgcg
    22381 ttggcgctgg ttgcccttgt tttcgttatc tggcgcgtag acagtgcgcc atcaactaat
    22441 gacgcttacg cgtcagcaga taccattgat gtggtgccgg aagtcagcgg tcgcattgta
    22501 gaactggcgg tcaccgacaa ccaggcagtc aaacagggcg atttgctgtt ccgcattgac
    22561 ccgcgcccgt acgaagccaa tctggcgaaa gctgaagcct ccctcgcggc gctggataag
    22621 caaattatgc tcacccagcg tagcgttgac gcgcaacagt ttggtgccga ctcggttaac
    22681 gccacggtag aaaaagcccg tgccgccgcg aaacaggcca cagatacatt acgccgcacc
    22741 gagccattac tgaaagaagg ttttgtctca gcggaagacg ttgaccgtgc gaggaccgcg
    22801 cagcgcgctg cagaagccga tcttaatgct gtattgttgc aggcgcagtc agccgccagc
    22861 gccgtcagcg gcgtggatgc attagttgcc cagcgtgcgg cggtcgaagc ggatattgcc
    22921 ctgaccaaac tgcatctgga aatggcgacc gttcgcgcgc cgtttgatgg ccgggtcatt
    22981 tccctcaaaa cctccgtcgg gcaatttgct tctgccatgc gccctatttt taccctaatc
    23041 gacactcgtc actggtacgt gatcgccaac ttccgcgaaa ccgatctgaa aaatattcgc
    23101 tcaggtacac ccgcaacgat tcgcctgatg agtgacagcg gcaaaacctt cgagggtaaa
    23161 gtggattcga ttggctacgg cgtgctaccg gatgacggcg gcctggtgct gggcggcctg
    23221 ccgaaagttt ctcgctctat taactgggtc cgcgttgccc agcgttttcc ggtcaaaatc
    23281 atggtcgata aacctgaccc ggaaatgttc cgcatcggcg cttcggcagt cgctaatctt
    23341 gagccgcaat aatgagcgcg ctcaactccc tgccattacc ggtggtcagg ctgctggcgt
    23401 tctttcatga agagttaagc gagcggcgac caggccgcgt gccgcagacc gtacaactct
    23461 gggtaggctg cctgctggtg attctgatct cgatgacctt tgagatccct tttgtggcgt
    23521 tatcgctggc agtgctgttt tacggtattc agtcgaacgc gttttacacc aaatttgtcg
    23581 cgatcttgtt tgtggttgcc acggtgctgg agatcggcag cctgtttttg atctacaaat
    23641 ggtcatacgg cgaaccgttg atccgattga tcatcgccgg gccgatcctg atgggctgta
    23701 tgtttttgat gcgcacccat cggctgggac tggtcttttt cgctgtcgcc attgtcgcca
    23761 tttacgggca aaccttcccc gccatgctcg actatccgga agtggtcgtg cgcttaacgc
    23821 tgtggtgtat cgttgttggc ctctatccaa ccttgctgat gacgttaatc ggcgtgctgt
    23881 ggtttcccag tcgtgccatt acgcaaatgc atcaagcgct taatgatcgg cttgatgatg
    23941 ccattagtca cctgacggac agcctcgcac cgctacccga aacgcggatt gaaagagagg
    24001 cgctggcgct gcaaaaactg aatgtctttt gtctcgcgga cgatgccaac tggcgaactc
    24061 aaagcgcatg gtggcaaagc tgcgtggcaa cggtaaccta catttactcg acgctgaatc
    24121 gctacgatcc cacctctttt gctgattctc aggcaattat tgaatttcgg caaaaattag
    24181 cctcagaaat caataagctg cagcatgccg ttgctgaagg tcagtgctgg caaagcgact
    24241 ggcggatcac tgaaagtgaa gcgatggcgg cacgggaatg taacctggag aatatctgcc
    24301 agacgttgtt acaactgggc cagatggacc cgaatacgcc gccaacgccc gccgccaaac
    24361 cgccatcaat ggtcgccgat gcttttacca atccagacta tatgcgctac gcggtaaaaa
    24421 cactgctcgc ctgtttgatc tgttacacct tctacagcgg cgtggactgg gaaggcattc
    24481 acacctgtat gctgacatgc gtgatcgtcg ctaacccaaa tgtcggttcg tcgtaccaga
    24541 agatggtgct gcgttttggc ggggcctttt gcggcgcgat tctggcgctg ttattcacgc
    24601 tactggtcat gccctggctg gacaatattg tcgaattgct gtttgtgctg gcaccgattt
    24661 tcctgttggg cgcatggatt gccaccagct ctgaacgctc ttcttatatc ggcacacaga
    24721 tggtggtcac cttcgcgctc gccacgctcg aaaacgtttt tggcccggtg tacgacctgg
    24781 tggaaattcg cgatcgcgcc atgggtatca tcattggtac cgtggtgtcc gcggtgattt
    24841 acacctttgt ctggcctgaa agtgaagcgc gcacactgcc gcaaaaactg gctggcacgc
    24901 tgggtatgtt aagtaaagta atgcggatcc cacgccagca ggaagtcacg gctctgcgca
    24961 cttatctgca aattcgtatc ggtctgcatg cggcgtttaa tacctgtgaa gagatgtgcc
    25021 aacgcgtggc gctggagcgt caactggaca gcgaagaacg cgccttactg attgaacgtt
    25081 cgcaaacggt tattcgtcag ggccgcgata ttcttcacgc ctgggatgcc acctggaact
    25141 cggcgcaggc gctggataac gcactacagc cggacagagc agctcagttt gccgacgccc
    25201 tggagaaata cgctgccggt ctggcaaccg cactcagccg ttctcctcaa ataacgcttg
    25261 aagagacacc cgcctcgcag gccatcctgc ccaccttatt aaaacaggag caacacgtct
    25321 gccagctttt cgcccgcttg ccagactgga cagccccggc attaacgccc gccacggaac
    25381 aggcacaagg agccacgcaa tgatcaatcg tcaactttca cgtctgctgt tgtgcagcat
    25441 tctcggcagc acgacgctga tttccggctg tgccctggta cgtaaggatt ctgcacctca
    25501 tcaacagctc aaaccggaac aaatcaaact ggccgacgat attcatcttg ccagctccgg
    25561 ctggccgcag gcgcagtggt ggaaacaact caatgacccg cagctggatg cgctgatcca
    25621 acggacgcta agtggttcac acaccctcgc cgaagcgaaa ctgcgggaag aaaaagcgca
    25681 gtcccaggcc gatttgttag atgccggttc acaattacag gtcgcagcgt tagggatgct
    25741 caaccgccaa cgcgtctcgg cgaacggctt tttaagccct tatgcgatgg atgcgcccgc
    25801 actgggtatg gacgggccgt actatacgga agccacagta ggtttgtttg ccggactgga
    25861 tcttgatttg tggggtgtgc atcgctcagc ggttgccgcc gccattggcg cgcataatgc
    25921 cgcgctggca gaaaccgcag cagtagagct atcgctgacc acgggcgtag cgcagcttta
    25981 ttacagtatg caggccagct atcagatgct cgatctgtta gaacaaactc gcgatgtgat
    26041 tgattacgcg gtgaaagcgc accagagtaa agtggcgcac ggtctggaag cacaagtgcc
    26101 tttccacggc gcgcgggcac agattctggc ggtcgataaa caaattgctg ccgtcaaagg
    26161 gcaaatcacc gaaacgcgag aatctctgcg tgcattgatt ggcgcggggg ccagcgatat
    26221 gccggagatc aaaccggtgg cattaccacg agtccagacc ggcattccgg cgacactctc
    26281 ttatgagttg ctcgccagac gcccggatct gcaagccatg cgctggtatg ttcaggcgtc
    26341 attagatcag gtggattccg cgcgggcgct gttctacccg agctttgata tcaaagcgtt
    26401 tttcggcctt gatgccatcc acctggatac cttattcaaa aaaaccagtc gccagttcaa
    26461 cttcatcccg ggtctgaaat tgccgctgtt tgacggtgga cggttgaatg ccaatctcga
    26521 aggcacgcgc gccgccagca acatgatgat tgaacgttac aaccagtcag tactgaacgc
    26581 ggtgcgtgac gttgccgtca acggcacgcg tctgcaaacg ctcaacgacg agcgagaaat
    26641 gcaggctgaa cgcgtggaag caacgcgctt cacccagcgc gctgccgagg ccgcctatca
    26701 gcgcggctta accagccgct tacaggccac tgaagcccgg ttgccagtac ttgccgaaga
    26761 gatgtcatta ctgatgctgg acagccgccg ggtgatccaa agcattcagt tgatgaaatc
    26821 gctgggcggc gggtatcagg cggctcccgt cgtcgagaaa aaataaaatg tctgccgcgt
    26881 gatggctgtc acgcggtatt tcgtttcgtc acgtcaaaac tgacgacagc ctgtttttcg
    26941 tcagagtttt gaataaatag tgcccgtaat atcagggaat gaccccaaat aaaatgtggc
    27001 ataaaagatg catactgtag tcgagagcgc gtatgcgtga tttgattaac tggagcgaga
    27061 ccgatgaaaa aagtcgtcac ggtttgcccc tattgcgcat caggttgcaa aatcaacctg
    27121 gtcgtcgata acggcaaaat cgtccgggcg gaggcagcgc aggggaaaac caaccagggt
    27181 accctgtgtc tgaagggtta ttatggctgg gatttcatta acgataccca gatcctgacc
    27241 ccgcgcctga aaacccccat gatccgtcgc cagcgtggcg gcaaactcga acctgtttcc
    27301 tgggatgagg cactgaatta cgttgccgag cgcctgagcg ccatcaaaga gaagtacggt
    27361 ccggatgcca tccagacgac cggctcctcg cgtggtacgg gtaacgaaac caactatgta
    27421 atgcaaaaat ttgcgcgcgc cgttattggt accaataacg ttgactgctg cgctcgtgtc
    27481 tgacacggcc catcggttgc aggtctgcac caatcggtcg gtaatggcgc aatgagcaat
    27541 gctattaacg aaattgataa taccgattta gtgttcgttt tcgggtacaa cccggcggat
    27601 tcccacccaa tcgtggcgaa tcacgtaatt aacgctaaac gtaacggggc gaaaattatc
    27661 gtctgcgatc cgcgcaaaat tgaaaccgcg cgcattgctg acatgcacat tgcactgaaa
    27721 aacggctcga acatcgcgct gttgaatgcg atgggccatg tcattattga agaaaatctg
    27781 tacgacaaag cgttcgtcgc ttcccgtaca gaaggctttg aagagtatcg taaaatcgtt
    27841 gaaggctaca cgccggagtc ggttgaagat atcaccggcg tcagcgccag tgagattcgt
    27901 caggcggcac ggatgtatgc ccaggcgaaa agcgccgcca tcctgtgggg catgggtgta
    27961 acccagttct accagggcgt ggaaaccgtg cgttctctga ccagcctcgc gatgctgacc
    28021 ggtaacctcg gtaagccgca tgcgggtgta aacccggttc gtggtcagaa caacgtgcag
    28081 ggtgcctgcg atatgggcgc gctgccggat acgtatccgg gataccagta cgtgaaagat
    28141 ccggctaacc gcgagaaatt cgccaaagcc tggggcgtgg aaagcctgcc tgctcatacc
    28201 ggttatcgca tcagcgagct gccgcaccgc gcagcgcatg gcgaagtgcg tgccgcgtac
    28261 attatgggcg aagatccgct gcaaactgac gcggagctgt cggcagtacg taaagccttt
    28321 gaagatctgg aactggttat cgttcaggac atctttatga ccaaaaccgc gtcggcggcg
    28381 gatgtcattt tgccgtcaac gtcgtggggc gagcatgaag gcgtgtttac tgcggctgac
    28441 cgtggcttcc agcgtttctt caaagcagtt gaaccgaaat gggatctgaa aacggactgg
    28501 caaatcatca gtgaaatcgc cacccgtatg ggttatccga tgcactacaa caacacccag
    28561 gagatctggg atgagttgcg tcatctgtgc ccggatttct acggtgcgac ttacgagaaa
    28621 atgggcgaac tgggcttcat tcagtggcct tgccgcgata cttcagatgc cgatcagggg
    28681 acttcttatc tgtttaaaga gaagtttgat accccgaacg gtctggcaca gttcttcacc
    28741 tgcgactggg tagcgccaat cgacaaactc accgacgagt acccgatggt actgtcaacg
    28801 gtgcgtgaag ttggtcacta ctcttgccgt tcgatgaccg gtaactgtgc ggcactggcg
    28861 gcgctggctg atgaacctgg ctacgcacaa atcaataccg aagacgccaa acgtctgggt
    28921 attgaagatg aggcattggt ttgggtgcac tcgcgtaaag gcaaaattat cacccgtgcg
    28981 caggtcagcg atcgtccgaa caaaggggcg atttacatga cctaccagtg gtggattggt
    29041 gcctgtaacg agctggttac cgaaaactta agcccgatta cgaaaacgcc ggaatacaaa
    29101 tactgcgccg tgcgcgtcga gccgatcgcc gatcagcgcg ccgccgagca gtacgtgatt
    29161 gacgagtaca acaagttgaa aactcgcctg cgcgaagcgg cactggcgta atacagtcct
    29221 ttctacagcc tcctttcgga ggctgttttt ttatccattc gaactcttta tactggttac
    29281 tccctaccca atcgtattat caaaatgaaa aaaattatcg cattgatgtt gtttttgaca
    29341 ttctttgccc acgcgaacga ctccgagcct ggcagccagt atttaaaggc agcagaggcc
    29401 ggggaccgac gcgcacaata ttttcttgcc gacagctggt ttagctccgg cgatttgagc
    29461 aaagccgaat attgggcaca gaaagccgcc gacagcggtg atgctgatgc ctgcgcgctg
    29521 ctggcgcaga tcaaaatcac caatccggtc agtctggact atccacaagc aaaagttctt
    29581 gcagagaaag cggcgcaagc gggcagtaaa gaaggtgaag taacgctggc gcatattctg
    29641 gtaaatactc aagcgggtaa accggattat ccaaaggcga tttcgctgtt agaaaacgcc
    29701 tcggaagatc tggagaatga ctccgccgtc gatgcccaaa tgctgcttgg tttgatttac
    29761 gccaacggcg tgggcattaa ggccgacgat gacaaggcaa cctggtattt caaacgcagc
    29821 tctgcaattt cccgaaccgg ttattccgag tactgggcgg gaatgatgtt cttaaacggt
    29881 gaggaaggtt ttatcgagaa gaacaaacaa aaggcgctgc actggttgaa cctgagctgt
    29941 atggaagggt ttgataccgg gtgtgaagag tttgaaaaat taaccaacgg ttaaggtagg
    30001 cctgataaga cgcaccagcg tcgcatcagg catccacaca ttgccgggtg gataaccccg
    30061 gcaatcttca attattgatc cgcagtttta tcaaatttgc ccagcacttc acgctcataa
    30121 gccagcgcct ttttgcggtc aaatttgtgt tcccacttgg caatcaccag caccgccagc
    30181 gcattaccca ccacgttcag cgcagtacgc gccatgtcga ggatacggtc aacaccagca
    30241 ataaacgcca ggccttccag cggaatacct acgctaccca gcgttgccag cagcaccaca
    30301 aacgacactc ccggcacgcc agcaatccct ttcgaggtca ccatcagcgt cagcaccaga
    30361 atgatttcct gccagatgga caggtcaatg ccatacagct gagcgatgaa aatagcggca
    30421 atactttgat acagcgtcga accatcaagg ttaaaagagt aaccggtcgg caccacgaag
    30481 ctggtgatcg acgccggtgc tccgtaggct tccatcttct caataattcg cggcagtacg
    30541 ctttcagagc tggcagtgga gtacgccaga atcagctcat ctttcagaat acgaatcagg
    30601 atccagacgc ttaacccgca caggcgcgcc acaattccca gcactaccag cgcgaagaac
    30661 agaatggcga aatgcaccag cagcaccagt ttcgccagtg gccacagaga agagaaacca
    30721 aagttagcca ccgtcaccgc aatcagcgca aacacaccca ccggtgcata acgcatcacc
    30781 atgtgagtca ctttaaacat ggtttcagag atggagcgga acacggtcac cagcggttca
    30841 cgatgcgtcg cgggcaggga ggaaagcccc agaccaaaca gcaccgagaa gaagatgatc
    30901 ggcagcattt cgcctttcgc catcgacgcc acaatgttcg tcggcaccag cgacaaaatc
    30961 gtgcccataa tgccgtggga actgctttgt accgcttccg tagtgctctg atatttcgag
    31021 atatcgacgg tcgccagctg cgacatatcc accccggcac cgggctggaa gacgttcgcc
    31081 agagtgatcc ccaaaatgat ggcgacggtg gtgatcacct cgaagtaaat aatggttttc
    31141 gcgccaatac gcccgagctg tttggcatca ccaacacccg cgatacccac caccagcgtg
    31201 gagatcacaa tcggcacaac aatcattttg atcagatgga tgaagatatc acccgccgga
    31261 gagagcaaat tgacgaccag ccagtcgcgg ctgtcgctat ggtagtgcag gtagcttccc
    31321 aggagaatgc ccagcaccat agcaaacaga atctgccagg ccaggctgaa ttttatactt
    31381 ttcatcagaa taacttcctc aatggagcgc cgttccccgt tcagaaacgc gagaacgtga
    31441 catactgcca gggcatgaat tgtgttgcgt tggtttgtag atttttttaa cgcttatgat
    31501 cagtatcatt tgcgcggtat gttgcgcaac cctttaacaa cagagctttt cgctgcatta
    31561 atgcataata acgcgagttt atggcaggat taatgaataa ccatttgtta tggaaagatt
    31621 tttatttgtt taatctgcaa taatactgat tggtgattat ttttggcata tgttcattcg
    31681 attatttttt atacaactca ataacataat cttcatcacc agatgattaa tctgatctcc
    31741 gctgcaacaa tttcgccata ttaatcgact cgaccagctg tgtccggttg atccggggtg
    31801 agttgagatc catcactttt tgccatagtt cgatggcttg cgcgtaattc gcctgcataa
    31861 acgcatcgga agccagcagc atcagagcgg tgatttcgtt agagtccagc gcgagggctt
    31921 tgtcgatcat tgcgcgagtc tgggcggtca tatgctggct ggcctggtaa taaagcaccg
    31981 tcgccagcgc cgcatacagt tcagcgttct caccacgtag ttgcaacgcc tgacggtacg
    32041 ccagcagcga attgctgtaa tcgttttgcc acagatagta ctcgcccagt aacgcccatt
    32101 gttcgctgtt ttgtggatta gcgcggattt tatcctgcaa tgcctgaagc tgcgcttccg
    32161 gggtttgctg gctggcaaac tgatgtagcg gatcgcgctg acgctgatat tccgcatgta
    32221 ccgcctgcca ttttggactt aacagataac tgccgacaca gagaaatacc atcaaaatcg
    32281 tgagtgttgt cagcattttc accggtattt tgggctcttt catcgcttcg ccctcactcg
    32341 ccagaggatc agtgccatca gcagtaacaa caccactggc agcgcccaca acaccagcgt
    32401 ctgacccgtt aacggtgggt tatagcgaac aaaatctccg tagcgttcgg tcatccagcc
    32461 aatgatttcg acttcgttct tcccctccgc caccatgctg taaacctgat ggcgcatact
    32521 gactgccacc ggcgcgttgg attccagtaa gttttgattc tggcactgcg gacaacgtaa
    32581 ctggctggca atatttaacg cctgttgctg ttgttgcgga ttggcgaatt gccaggtgtc
    32641 tacgacctga gcgcgcgcaa aacaggtaaa taacagcagt agcgtgagaa gtcctttatt
    32701 catcgcgctt cctcccccgc catccgctta acaatgcgcc cgcaatcatc aacaatcctc
    32761 ccccccagat ccagcgcaca ccgctttgta catacaagcg aaaagcgtaa cgatccgctc
    32821 cggttttttc acccatgacc gcataccagt catggatgcc gttccagcga attgacggtt
    32881 ccatcatttg ctgacgacgt gcttcgtaaa aacgccgctc cggcatcagt tcaccaatgc
    32941 gttgctgatg gtcaaacagt gccactatcg ctttttcgct ggtgtaattg cctttggctt
    33001 gcagatcgag gcgctcaaaa cggaaggtgt atcctgccag cgtcacctgc tgacccggct
    33061 gtaaattcag gctgatctcc tgacggctga cgctggaaac cacgatcccc gcggcaaata
    33121 acagcacgcc cgcatgagct accagcgccg gaagctgcac gcgtttgcca gagacaaacg
    33181 tcgccagcac aatcaccacc agcatcaaca ggccaaacgg taacgtcgcg cggttaaaat
    33241 acggcgcgcc aacggagagg cgtccccagc ccagcaggcc gtaaatcatc ggataaagcg
    33301 ttcccaccag cacgatcagt agcactgcgc aaaacagcaa cagcgtagcg aggattaaca
    33361 tttcccgcga taaccccgaa aaacgcacca ccgcaccacc gtcccgcgct cgccagccat
    33421 acagagccag agacgcaagg ctaatcagtg caaacaggct gaacaacggc acggcgcgga
    33481 cgttatccag tgcgaacgca tgaaccgaaa ccagaatgcc agaacggaca attaaggtac
    33541 ccagtagcga cagcatcaga gttactatcg ccagcaacag cgaccagtgg cggaaaatct
    33601 cccgctggcg tgtcagggac aaactgtgca gcagcgcagt ggcagaaagc cagggtaata
    33661 aagaggcgtt ttccaccgga tcccagaacc accagccgcc ccagcccagt tcgcaatagg
    33721 cccaccaaga accgaggatg atccccgccg ttaatgcgct ccagccaggt agtgcccagc
    33781 gccaacaaat tctggcgcag gcaccatcaa actcgccgcg cagtaaactc gccagcgcca
    33841 cgctcgccgc taccatcaaa ccgccatagc caaggtaaag cagcggtgga tgaaagataa
    33901 gaccgggatg ttgcagcatc ggattgagat cgcggccttc gattgctggt ggaaatatcc
    33961 gcacgaaggg atcggaccac agcaccacaa acagtagcag tgcggcgagc atgagagata
    34021 aaacggctaa cgtcagcgga aatagcggat cggtttgctg ccgataatgc caggcaaaca
    34081 gcgcgctcca ggcggaaagc agcagcaccc aaagcagcag cgaaccttca tggccgcccc
    34141 acaccgctgc cagcttgagt tcccacgaca gcaggctata gctatgttgc gcgacataaa
    34201 tgaccgagaa atcgctgatg agaaaacaat acgtcagtac gccaaaggcg agcagcagga
    34261 gcgcgaactg tgccagaatg ccgatgcaag tgagtcgcat catggcaggc cagcgcaacc
    34321 gcactcccgc gaaggccgtc aacggggtca acacgttgac cccgagactt aacaacaacg
    34381 ccagaaaacc gacttcagga aggaatacgt ccagaaacca cctcttttct ggcttacgcc
    34441 accgtcaact gtccggcgta gagaatgaaa aagcgcagca tcaacacgcc gaccagactg
    34501 gccccacacg ccgccagcac ggcaggaatg ccggaactgc gattgaccca cggtttgagc
    34561 agcattggca caatcagccc cagcccggcg acaccaagcc agaaccacca cgtccagaaa
    34621 ccgccaccta atgccgccac cagcgcacgc actttaccgt catcgcccag ggccagaccg
    34681 acaaaaaacg ccaccagcag gaagatttca ccccatacca cagggatttc catacggtga
    34741 acaaagtgcg cttccgtgga atgcggattt ttgcgttggc gtatcgccat ggcgatcagc
    34801 gctaccgcca caccggacga gatgccggag aagaggaaca gcaccggcag gatcgggtta
    34861 ttgaggaacg gatacgattt cagcgccgac agcagaaagc cggtataagc ccccagcagc
    34921 accgccaaca ccagcatcag cgtttccagt ccgcgatgaa cgggcgtcag caggctcaga
    34981 accttttgca cgatccccag cttcggcaac cagcgttgtt gcagggcaag caaatcatgt
    35041 tcaaagattt tcgccagcca cagcaccagc accaccatgt agagctgaaa cagcatcacc
    35101 cccatcgaca tcaccgaggt aaaactgtag tggaacatca gcttccagaa ggtccacggt
    35161 cttgtcaggt ggaagacgag gatcaacaga ccgaggatca ccgcgccagg tccgacaatc
    35221 agcgtggtgc gcagcaacgt gctgtctgca ccgcccgcct gcgggtagaa gcgacgtaac
    35281 agcacggcca gcgtcaccag accggcagaa ataccaatca aaaacaggta gatggcaatc
    35341 ggccagtccc acaccagcga ttcaaaatga aatgcggaag tctgcgtcat tggctcacct
    35401 ccccgtattt aaacggtacg cggtacagct tcggtttggt tcccagcgcc agcttgtagc
    35461 ggtaagtagg cttctggcgc agcagttgcg aaatctcact gttaggatcg tccagattgc
    35521 caaacgtcag cgccttcgtc gggcaagcct caacgcacgc gggcagctta ccggcctgca
    35581 aattggtctt acggcagaaa tcgcatttgt ccgccgtctt cgtgaccgga tggataaagc
    35641 gcacgcggta cggacaggcg gcgatgcagt actgacaacc gacgcagaga tcaggattaa
    35701 catcgacaat cccgctagcg gcatcacgaa aagacgcacc ggtcgggcag acgtcaacgc
    35761 acggcgcatg atcgcagtgc tggcaagact tacggaagaa gcgatatttc acatcaggaa
    35821 attcgccctg cggctcgcta cgaataatcg tcaggcgcga gacgccttcc ggcaccttgt
    35881 tcacttcccg acaagcatcc atacaggcgg tacagccgat gcataacgac tcgtcatgca
    35941 ccatgccgta acgcacccca ttgatattca acgttttcgc caccacgcgc cctgcggtgc
    36001 cgctgacggt tgccagcacg ccgacgccgg tgagaaactg gcgacgagac caggtcatgg
    36061 ctgctcctta agcaacggaa cagacgccgg gttaaagttc ggattggtgc gctgatcgct
    36121 gtggcaatcg acgcaaatct taatccgtcc tttgtcactt aacgtctgca tcgtatcttg
    36181 ctgtggatgc agggaatggc agctggcgca cgccactttg gttacgtgga catcgtgcgg
    36241 ccagaacgct ttttgcaact gttccggcag atgacaggac atacagacgc tgttctgctc
    36301 tccgaccttg tacatcggct cgttaaagcg catcacatct ttcacccctt cgcggtgttg
    36361 cggtgatggc tggccgtgac agttggtgca ggtgaccggc agtttgttat tcgggttgat
    36421 aacggaagca tgtttgccat gcataccttc gatatctggc ttatgacagt ccagacaggc
    36481 ggcatccggg ttacgctgct gggtaacttc gtagcgatca ccagatgcct gcgccgcagg
    36541 ggtagcggta attccgttca ggctccacaa caggcccgac gccagcaccc cagcagttaa
    36601 taacgaacgt aatacgctca tattcactcc attgtgtttt ccgccccttg cgaggcggaa
    36661 ctgggttatt ggcttaacag accattttta cgtgcctgtt cttcccactg cgggatcacc
    36721 gttttgatga agtcctgctt ctcggccttg atttgttcca tgttcaggcc aatggcctgc
    36781 tgggctttct ctttggtcga gatatccggg atctggattt catgggtgat gcctttggtc
    36841 gccagcaggc gcgccagttt ggtgcgtgca tccgccgctt tatccatcgc cgtaccgagc
    36901 atccgtaaac cttcttccgg tgcgtgcata tgaatgccgt gggaagcgat tgccagatcc
    36961 cagcgccact gggcatgacg gatatcgtcc tgaattggct tcatttcagc ttccgtcgcg
    37021 cctgcatcca gcgctgcttt cgcttcgaag tgagcgtgaa ccagttgatc ttcaaccttg
    37081 attttcaggt cgttaatcga ctgcttacgt tccgcgacca ctttttgcaa ggcagctttg
    37141 tcctgggtat ggcagttcgc acaagtctgg gcgaagttat caaacggatt accaatttta
    37201 tggtcggtgt agagtttgcc ttcggcgttc tgcacttttg gcatatggca gtcgatacag
    37261 gtcacgttgt ttttaccgtg aatgcccgct gtccaggttt catattccgg gtgctgcgct
    37321 ttcagcattg gcgttttcga cagggagtta gtccagtcag agaaggcaat tttgtcgtaa
    37381 tactgctcca tattttcgac tttcatgccg tcatcccacg ggaatttaac cgctttgttt
    37441 ttgccgtcga agtaatactc cacatggcac tgaccgcaaa ccatcgattg ctggtcgaaa
    37501 cgtccggctt tctcaaaagg tttaccaatg gcttccatcg cgcgagccgc atacggacgg
    37561 gaaagggtta actccggttt gcctttggcg aactctggtg aggcggtgtt atggcagtcg
    37621 gcacaaccta agttgttgac gatttccggg ccgccgcgcg cccatttacc gtggaagtag
    37681 ccatcttcgc cgtctttctg gatcagacgc gccacatcag ggcttttaca actccagcat
    37741 gccatcggta gcggaccatc ttcagcgttt ttcggcgcac cggtacgcag ggtttcacgc
    37801 acatcggtca cagcaaaggc atgtccacgc ggcttgttgt aatcgcgcga gaagggatac
    37861 cccgcccaca ggatcaccag ccgtggatct tccgccaggg cgtcaacacg ctctgactgt
    37921 tccgaggtgg ctttccagga gagatattga tcgggatgct gcggggcaaa ggtttcattc
    37981 ttcgcttcca cagttacagg ttttgcggga gcagccgttt gttcagcgtg aacagaagtg
    38041 aaaaagaaaa aaggaatcaa taagctgaag atacggcgtg cgtttatttt tatccttgtc
    38101 ataggggctt catccgaatt gcgccattgt tgcaatggcg gtttttattg tttttcacga
    38161 cagtaaccgc acctacactg tcatgacatt gctcgcccct atgtgtaaca aataaccaca
    38221 ctgtgaatgt tgtctttaat caattgtaag tgcatgtaaa ataccacttt agagttagtc
    38281 agtatcttcc tctttttcaa cagcatgcat aactgcatgt tcctcaaaga attaatcaac
    38341 ttttgttgct gaccttcaaa aattaccctg ccgtttattt gcacaattct acttttgcgt
    38401 gatctgtcgc ccaaatacta aacaaaactg ccaatacccc tacatttaac gcttatgcca
    38461 catattatta acatcctaca aggagaacaa aagcatgagc caaattcaca aacacaccat
    38521 tcctgccaac atcgcagacc gttgcctgat aaaccctcag cagtacgagg cgatgtatca
    38581 acaatctatt aacgtacctg ataccttctg gggcgaacag ggaaaaattc ttgactggat
    38641 caaaccttac cagaaggtga aaaacacctc ctttgccccc ggtaatgtgt ccattaaatg
    38701 gtacgaggac ggcacgctga atctggcggc aaactgcctt gaccgccatc tgcaagaaaa
    38761 cggcgatcgc accgccatca tctgggaagg cgacgacgcc agccagagca aacatatcag
    38821 ctataaagag ctgcaccgcg acgtctgccg cttcgccaat accctgctcg agctgggcat
    38881 taaaaaaggt gatgtggtgg cgatttatat gccgatggtg ccggaagccg cggttgcgat
    38941 gctggcctgc gcccgcattg gcgcggtgca ttcggtgatt ttcggcggct tctcgccgga
    39001 agccgttgcc gggcgcatta ttgattccaa ctcacgactg gtgatcactt ccgacgaagg
    39061 tgtgcgtgcc gggcgcagta ttccgctgaa gaaaaacgtt gatgacgcgc tgaaaaaccc
    39121 gaacgtcacc agcgtagagc atgtggtggt actgaagcgt actggcggga aaattgactg
    39181 gcaggaaggg cgcgacctgt ggtggcacga cctggttgag caagcgagcg atcagcacca
    39241 ggcggaagag atgaacgccg aagatccgct gtttattctc tacacctccg gttctaccgg
    39301 taagccaaaa ggtgtgctgc atactaccgg cggttatctg gtgtacgcgg cgctgacctt
    39361 taaatatgtc tttgattatc atccgggtga tatctactgg tgcaccgccg atgtgggctg
    39421 ggtgaccgga cacagttact tgctgtacgg cccgctggcc tgcggtgcga ccacgctgat
    39481 gtttgaaggc gtacccaact ggccgacgcc tgcccgtatg gcgcaggtgg tggacaagca
    39541 tcaggtcaat attctctata ccgcacccac ggcgatccgc gcgctgatgg cggaaggcga
    39601 taaagcgatc gaaggcaccg accgttcgtc gctgcgcatt ctcggttccg tgggcgagcc
    39661 aattaacccg gaagcgtggg agtggtactg gaaaaaaatc ggcaacgaga aatgtccggt
    39721 ggtcgatacc tggtggcaga ccgaaactgg cggtttcatg atcacgccgc tgcctggcgc
    39781 taccgagctg aaagccggtt cggcaacacg tccgttcttc ggcgtgcaac cggcgctggt
    39841 cgataacgaa ggtaacccgc tggaaggcgc taccgaaggc agcctggtga tcaccgactc
    39901 ctggccgggt caggcgcgta cgctgtttgg cgatcacgaa cgttttgaac agacctactt
    39961 ctccaccttc aaaaatatgt atttcagcgg cgacggcgcg cgtcgcgatg aagatggcta
    40021 ttactggatc accgggcgtg tggacgacgt gctgaacgtc tccggtcacc gtctggggac
    40081 ggcagagatt gagtcggcgc tggtggcgca tccgaagatt gccgaagccg ccgtagtagg
    40141 tattccgcac aatattaaag gtcaggcgat ctacgcctac gtcacgctta atcacgggga
    40201 ggaaccgtca ccagaactgt acgcagaagt ccgcaactgg gtgcgtaaag agattggccc
    40261 gctggcgacg ccagacgtgc tgcactggac cgactccctg cctaaaaccc gctccggcaa
    40321 aattatgcgc cgtattctgc gcaaaattgc ggcgggcgat accagcaacc tgggcgatac
    40381 ctcgacgctt gccgatcctg gcgtagtcga gaagctgctt gaagagaagc aggctatcgc
    40441 gatgccatcg taacccacaa ttgccggatg cgagtcggta acggtttgta ggcctgataa
    40501 gacgcgacag cgtcgcatca ggcattgatt gccggatgcg gcgtaaacgc cttatccggc
    40561 ctacattcgg caagggttac ccgagcgtta accttctccc ataagggagc gggaattaaa
    40621 acaatcccta cattacctct ggagaatctg tgatgaatgg cactatttat cagcggatag
    40681 aagacaatgc gcatttcagg gagttagtcg aaaaacggca acggtttgcc accatcctgt
    40741 cgattattat gctggcagtt tatatcggct ttattttact gatcgccttc gcgcccggct
    40801 ggctgggcac gccgctgaat ccgaacacca gcgtcacacg cggtattcca attggtgttg
    40861 gagtgattgt gatctccttt gttctcaccg gtatctacat ctggcgggcg aacggcgaat
    40921 tcgaccgtct taataatgaa gtcctgcatg aggtacaagc atcatgaaaa gagttctgac
    40981 ggcgcttgcc gccacactcc ctttcgcagc taacgccgcg gatgctatta gcggggccgt
    41041 agagcgccag ccaacgaact ggcaggcgat tattatgttc ctgattttcg tcgtgtttac
    41101 gctcggcatt acctactggg catcaaaacg cgtacgttct cgtagcgact actacaccgc
    41161 aggcggcaat atcactggct tccagaacgg gctggcgatt gccggggact atatgtccgc
    41221 cgcctcattc ttggggatct ccgcgctggt gtttacctcc ggctatgacg gcttaattta
    41281 ctcgctgggc ttcctggtgg gctggccgat cattttgttc ctgattgccg aacgtctgcg
    41341 taacctgggg cgctacacct ttgccgatgt ggcctcttac cgtctgaaac aagggccgat
    41401 tcgtattctt tcggcctgtg gttctctggt ggtggtggcg ctttacctta tcgcccagat
    41461 ggtgggcgca ggtaaactga tcgagctgct gtttggcctt aactatcaca ttgcggtggt
    41521 gctggtcggc gtgctgatga tgatgtacgt cctgttcggc ggcatgctgg cgaccacttg
    41581 ggtacagatt atcaaagccg tgctgttgct gttcggtgcc agctttatgg cctttatggt
    41641 gatgaaacac gtcggcttta gcttcaacaa tctgttcagt gaagcgatgg cggtacaccc
    41701 gaaaggtgtc gacatcatga agccgggcgg gctggtgaaa gatccgatct ccgcgctctc
    41761 tctgggtctg ggactgatgt ttggtacggc gggcttgccg cacattctga tgcgcttctt
    41821 tacagtcagc gatgcccgcg aagcacgtaa gagcgtgttc tacgccaccg gatttatggg
    41881 ctacttctat attctgacct ttattatcgg cttcggcgcg atcatgctgg ttggtgcgaa
    41941 tccggaatat aaagacgcgg cgggccatct gattggtggt aacaacatgg cggccgttca
    42001 cctggcgaat gcagtgggcg gcaacctgtt cctcggtttt atttcagcgg ttgcttttgc
    42061 caccattctt gcggtggttg ctggtctgac gctggcgggc gcatcagcgg tttcgcatga
    42121 cctgtacgct aacgtcttca aaaaaggcgc gaccgaacgt gaagaactgc gggtatcgaa
    42181 aatcaccgtg ctgattttag gtgtgattgc cattatcctc ggcgtgctgt ttgagaatca
    42241 gaacatcgcc ttcatggtag gcctggcgtt tgccatcgca gcgagctgta acttcccgat
    42301 cattctgctt tctatgtact ggtcgaaact gaccactcgt ggcgcgatga tgggtggctg
    42361 gctggggctg attaccgcag tggtactgat gatcctcggc ccgacgattt gggtacagat
    42421 ccttggtcac gaaaaagcca tcttcccgta tgaatacccg gcgctgttct ctatcagcgt
    42481 ggcattcctc ggcatctggt tcttctcggc aaccgataac tcagcggaag gtgcgcgcga
    42541 gcgtgaactg ttccgtgcac agtttatccg ctcccagacc ggctttggcg ttgagcaagg
    42601 ccgcgcacat taatctctcc ccttccccgg tcgcctgacc ggggaatact cttcctctcc
    42661 agcatgcatc accttttccc ataatattaa a
//
LOCUS       DAZGXZ010000024        41232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000024 DAZGXZ010000000
VERSION     DAZGXZ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.024"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            483..3668
                     /gene="rne"
                     /locus_tag="ACHKJF_001479"
     CDS             483..3668
                     /gene="rne"
                     /locus_tag="ACHKJF_001479"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP0948861.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3865..4818)
                     /gene="flgL"
                     /locus_tag="ACHKJF_001480"
     CDS             complement(3865..4818)
                     /gene="flgL"
                     /locus_tag="ACHKJF_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP0948862.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4830..6473)
                     /gene="flgK"
                     /locus_tag="ACHKJF_001481"
     CDS             complement(4830..6473)
                     /gene="flgK"
                     /locus_tag="ACHKJF_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP0948863.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6539..7480)
                     /gene="flgJ"
                     /locus_tag="ACHKJF_001482"
     CDS             complement(6539..7480)
                     /gene="flgJ"
                     /locus_tag="ACHKJF_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP0948864.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(7480..8577)
                     /gene="flgI"
                     /locus_tag="ACHKJF_001483"
     CDS             complement(7480..8577)
                     /gene="flgI"
                     /locus_tag="ACHKJF_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP0948865.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(8589..9287)
                     /gene="flgH"
                     /locus_tag="ACHKJF_001484"
     CDS             complement(8589..9287)
                     /gene="flgH"
                     /locus_tag="ACHKJF_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP0948866.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9340..10122)
                     /gene="flgG"
                     /locus_tag="ACHKJF_001485"
     CDS             complement(9340..10122)
                     /gene="flgG"
                     /locus_tag="ACHKJF_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP0948867.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(10260..11015)
                     /gene="flgF"
                     /locus_tag="ACHKJF_001486"
     CDS             complement(10260..11015)
                     /gene="flgF"
                     /locus_tag="ACHKJF_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP0948868.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(11035..12240)
                     /gene="flgE"
                     /locus_tag="ACHKJF_001487"
     CDS             complement(11035..12240)
                     /gene="flgE"
                     /locus_tag="ACHKJF_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP0948869.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLAFEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(12265..12960)
                     /gene="flgD"
                     /locus_tag="ACHKJF_001488"
     CDS             complement(12265..12960)
                     /gene="flgD"
                     /locus_tag="ACHKJF_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP0948870.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(12972..13376)
                     /gene="flgC"
                     /locus_tag="ACHKJF_001489"
     CDS             complement(12972..13376)
                     /gene="flgC"
                     /locus_tag="ACHKJF_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP0948871.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13380..13796)
                     /gene="flgB"
                     /locus_tag="ACHKJF_001490"
     CDS             complement(13380..13796)
                     /gene="flgB"
                     /locus_tag="ACHKJF_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP0948872.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            13951..14610
                     /gene="flgA"
                     /locus_tag="ACHKJF_001491"
     CDS             13951..14610
                     /gene="flgA"
                     /locus_tag="ACHKJF_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP0948873.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            14686..14979
                     /gene="flgM"
                     /locus_tag="ACHKJF_001492"
     CDS             14686..14979
                     /gene="flgM"
                     /locus_tag="ACHKJF_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP0948874.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            14984..15400
                     /gene="flgN"
                     /locus_tag="ACHKJF_001493"
     CDS             14984..15400
                     /gene="flgN"
                     /locus_tag="ACHKJF_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP0948875.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(15440..16975)
                     /gene="murJ"
                     /locus_tag="ACHKJF_001494"
     CDS             complement(15440..16975)
                     /gene="murJ"
                     /locus_tag="ACHKJF_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP0948876.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(17085..18008)
                     /gene="yceM"
                     /locus_tag="ACHKJF_001495"
     CDS             complement(17085..18008)
                     /gene="yceM"
                     /locus_tag="ACHKJF_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0948877.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(18010..18657)
                     /gene="yceH"
                     /locus_tag="ACHKJF_001496"
     CDS             complement(18010..18657)
                     /gene="yceH"
                     /locus_tag="ACHKJF_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP0948878.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(18668..19252)
                     /gene="rimJ"
                     /locus_tag="ACHKJF_001497"
     CDS             complement(18668..19252)
                     /gene="rimJ"
                     /locus_tag="ACHKJF_001497"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0948879.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            19488..20696
                     /gene="mdtH"
                     /locus_tag="ACHKJF_001498"
     CDS             19488..20696
                     /gene="mdtH"
                     /locus_tag="ACHKJF_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP0948880.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            20760..21407
                     /gene="grxB"
                     /locus_tag="ACHKJF_001499"
     CDS             20760..21407
                     /gene="grxB"
                     /locus_tag="ACHKJF_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP0948881.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            21541..22101
                     /gene="yceB"
                     /locus_tag="ACHKJF_001500"
     CDS             21541..22101
                     /gene="yceB"
                     /locus_tag="ACHKJF_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0948882.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            22207..23253
                     /gene="pyrC"
                     /locus_tag="ACHKJF_001501"
     CDS             22207..23253
                     /gene="pyrC"
                     /locus_tag="ACHKJF_001501"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP0948883.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            23327..23572
                     /gene="dinI"
                     /locus_tag="ACHKJF_001502"
     CDS             23327..23572
                     /gene="dinI"
                     /locus_tag="ACHKJF_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP0948884.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            23862..24116
                     /gene="bssS"
                     /locus_tag="ACHKJF_001503"
     CDS             23862..24116
                     /gene="bssS"
                     /locus_tag="ACHKJF_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP0948885.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            24231..25349
                     /gene="solA"
                     /locus_tag="ACHKJF_001504"
     CDS             24231..25349
                     /gene="solA"
                     /locus_tag="ACHKJF_001504"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP0948886.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIHHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            25397..25510
                     /locus_tag="ACHKJF_001505"
     CDS             25397..25510
                     /locus_tag="ACHKJF_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP0948887.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            25771..26337
                     /gene="yceJ"
                     /locus_tag="ACHKJF_001506"
     CDS             25771..26337
                     /gene="yceJ"
                     /locus_tag="ACHKJF_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0948888.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            26341..26916
                     /gene="yceI"
                     /locus_tag="ACHKJF_001507"
     CDS             26341..26916
                     /gene="yceI"
                     /locus_tag="ACHKJF_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP0948889.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(26958..28010)
                     /gene="trhO"
                     /locus_tag="ACHKJF_001508"
     CDS             complement(26958..28010)
                     /gene="trhO"
                     /locus_tag="ACHKJF_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP0948890.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            28235..29155
                     /gene="lpxL"
                     /locus_tag="ACHKJF_001509"
     CDS             28235..29155
                     /gene="lpxL"
                     /locus_tag="ACHKJF_001509"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP0948891.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            29327..30553
                     /gene="mdtG"
                     /locus_tag="ACHKJF_001510"
     CDS             29327..30553
                     /gene="mdtG"
                     /locus_tag="ACHKJF_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP0948892.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            30636..31010
                     /gene="msyB"
                     /locus_tag="ACHKJF_001511"
     CDS             30636..31010
                     /gene="msyB"
                     /locus_tag="ACHKJF_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0948893.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(31011..31238)
                     /gene="yceK"
                     /locus_tag="ACHKJF_001512"
     CDS             complement(31011..31238)
                     /gene="yceK"
                     /locus_tag="ACHKJF_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0948894.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(31411..33954)
                     /gene="mdoH"
                     /locus_tag="ACHKJF_001513"
     CDS             complement(31411..33954)
                     /gene="mdoH"
                     /locus_tag="ACHKJF_001513"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP0948895.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(33947..35482)
                     /gene="mdoG"
                     /locus_tag="ACHKJF_001514"
     CDS             complement(33947..35482)
                     /gene="mdoG"
                     /locus_tag="ACHKJF_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP0948896.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            35876..37033
                     /gene="mdoC"
                     /locus_tag="ACHKJF_001515"
     CDS             35876..37033
                     /gene="mdoC"
                     /locus_tag="ACHKJF_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP0948897.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(37041..38522)
                     /gene="clsC"
                     /locus_tag="ACHKJF_001516"
     CDS             complement(37041..38522)
                     /gene="clsC"
                     /locus_tag="ACHKJF_001516"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP0948898.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(38464..38997)
                     /gene="ymdB"
                     /locus_tag="ACHKJF_001517"
     CDS             complement(38464..38997)
                     /gene="ymdB"
                     /locus_tag="ACHKJF_001517"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP0948899.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHSVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(39092..39403)
                     /gene="ymdA"
                     /locus_tag="ACHKJF_001518"
     CDS             complement(39092..39403)
                     /gene="ymdA"
                     /locus_tag="ACHKJF_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP0948900.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(39524..39856)
                     /gene="csgC"
                     /locus_tag="ACHKJF_001519"
     CDS             complement(39524..39856)
                     /gene="csgC"
                     /locus_tag="ACHKJF_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP0948901.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(39915..40370)
                     /gene="csgA"
                     /locus_tag="ACHKJF_001520"
     CDS             complement(39915..40370)
                     /gene="csgA"
                     /locus_tag="ACHKJF_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP0948902.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(40411..40866)
                     /gene="csgB"
                     /locus_tag="ACHKJF_001521"
     CDS             complement(40411..40866)
                     /gene="csgB"
                     /locus_tag="ACHKJF_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP0948903.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            complement(40995..>41232)
                     /locus_tag="ACHKJF_001522"
     CDS             complement(40995..>41232)
                     /locus_tag="ACHKJF_001522"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0948904.1"
                     /translation="SAPPWTIFYCKKTRFVTACAQDISQQSATGKKNDCLVIFDSGKR
                     RFKRKQNIFCVDNSVFTWVLILWYEQKKKNTTRG"
BASE COUNT        10006 a        10837 c        10230 g        10159 t
ORIGIN      
        1 tcactttatt gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag
       61 cgggtgataa atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc
      121 cgtttccgtg tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga
      181 ccgatcatcc acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg
      241 cgggttgcag tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt
      301 ctttgataat tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt
      361 tgagctgacg ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac
      421 ccggaaaggc gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta
      481 cgatgaaaag aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag
      541 atgggcagcg tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa
      601 acatctacaa aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt
      661 acggcgctga acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg
      721 ctaactacag tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag
      781 tcattgttca gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta
      841 tcagtctggc gggtagctat ctggttctga tgccgaacaa cccgcgcgcg ggtggcattt
      901 ctcgccgtat cgaaggcgac gaccgtaccg aattaaaaga agcactggca agccttgaac
      961 tgccggaagg catggggctt atcgtgcgca ccgctggcgt cggcaaatct gctgaggcgc
     1021 tgcaatggga tttaagcttc cgtctgaaac actgggaagc catcaaaaaa gccgctgaaa
     1081 gccgcccggc cccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg
     1141 attacttacg tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg
     1201 cacgtcagca tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca
     1261 ccggcgagat cccgctgttc agccactacc agatcgagtc gcagatcgag tccgccttcc
     1321 agcgtgaagt ccgtctgcca tccggtggtt ccattgttat cgacagcacc gaagcgttaa
     1381 cggccatcga catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaaccgcgt
     1441 ttaacaccaa cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg
     1501 gcggcctgat tgttatcgac ttcatcgaca tgacgcctgt acgccaccag cgtgcggtag
     1561 aaaaccgtct gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt
     1621 ctcgctttgg cctgctggaa atgtcccgtc agcgccttag cccatcactg ggtgaatcca
     1681 gtcatcacgt ttgtccgcgt tgttctggta ctggcaccgt gcgtgacaac gaatcgctgt
     1741 cgctctctat tctgcgtctg atcgaagaag aagcgctgaa agagaacacc caggaagttc
     1801 acgccattgt tcctgtgcca atcgcttctt acctgctgaa tgaaaaacgt tctgcggtga
     1861 atgccattga aacgcgtcag gacggtgttc gctgcgtgat tgtgccaaac gatcagatgg
     1921 aaaccccgca ctaccacgtg ttgcgcgtgc gcaaagggga agaaacgccg accttaagct
     1981 acatgctgcc gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg
     2041 aacgtaagcg tccggaacaa cctgcgctgg caacctttgc catgccggat gtgccgccag
     2101 cgccaacgcc agctgaacct gccgcgcctg ttgtagcccc agcaccgaaa gctgcaccgg
     2161 caacaccagc aactcctgca caacctgggc tgttgagccg cttcttcggc gcactgaaag
     2221 cgctgttcag cggtagtgaa gaaaccaaac cgaccgagca accagcaccg aaagcagaag
     2281 cgaaaccgga acgtcaacag gatcgtcgca agcctcgtca gaacaaccgc cgtgaccgta
     2341 atgagcgccg cgacacccgt agcgaacgta ctgaaggcag cgataatcgc gaagaaaacc
     2401 gtcgtaatcg tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcaag
     2461 ctgaggtaac ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta
     2521 gccgccgccg taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg
     2581 aagagcaatc tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc
     2641 gtaaacagcg tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg
     2701 tagtcgcacc ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag
     2761 ctccacgcac agaactggtg aaagtcccgc tgccagtcgt agcgcaaacc gcaccagaac
     2821 agcaagaaga gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc
     2881 gctcgcctcg tcacctgcgc gtgagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt
     2941 atccaaccca gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg
     3001 gcaaagtctg gatccgctat ccaattgtac gtccgcaaga tgtacaagtt gaagagcagc
     3061 gcgaacagga agaagtacaa gtgcagccga tggtgactga ggtccctgtc gccgccgctg
     3121 tcgaacctgt tgttagcgcg ccagttgttg aagaaatggc tgaagtcgta gaagcccccg
     3181 taccggttgc cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgattgccg
     3241 ccgcggtaac tgaacagccg caggtgatta ccgagtctga tgttgccgta gcccaggaag
     3301 tcgcagaaca cgcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag
     3361 ttgccgaaac tgctgaggtt gtggttgctg aacctgaagt tgttgctcaa cctgccgcgc
     3421 cagtcgtcgc tgaagtcgca gcagaagttg aaacggtagc cgcggttgaa ccagagatca
     3481 ccgttgagca taaccacgct accgcgccaa tgacgcgcgc tccggcaccg gaatatgttc
     3541 cggaggcacc gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg
     3601 ccgcaggtgg tcatacggca acgcatcatg cctctgccgc tcctgcgcgt ccgcaacctg
     3661 ttgagtaata attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc
     3721 atctttgaat caacatatta aaattcatca aatggcatcc ttgctaacca acaatgcaaa
     3781 ataggcaaaa aaaatgcccg ccccggcgat cccagagcag gcagacaaaa catacccagt
     3841 ttcatgatat gttttaaagc gagcttattt gttgagctgg aagagcgaca atccctgcat
     3901 atcggtaaat gctttatacg atgcctgcaa tgccgtttgc tgcatgatgt aagatgaaat
     3961 agttgcattc cagtccacat caaccagatc gctcatctgc tgcgtttgcc ctaaagcgcg
     4021 atcgctacct aaggaatcca gcgactccag ttcgttcagc tgcgtgccta attccgcgcg
     4081 aacagtcagc acattgttca gcgagttttt cagtccgcgg ttggttttat ctaacgccgc
     4141 agcggcggtt tctttatccg cttcgctatc cgcgaccggc gttttcaggg ctgcgatggc
     4201 actatccagc atggcaaaaa gattggtttc agaagcgcta ccgtctggtt ccgctaccgc
     4261 gttgctggta atactgtcga aaattttgtc acccgtgtgc cctatcacca tcgaacgcga
     4321 agcatcgacc tgttgtttaa tactttctgc tccaccgacg tatttccctt tctcttcgct
     4381 aaacggcgca gtctctgttt tataaccggc aaaaatgtag cgcccgttac cgtcagtggt
     4441 gttcgccaga ttcagcaact ggtcacgaag cccctgaata tccgtagcca gcgaggcccg
     4501 gtcatcgtca ctcaaggtgc cattgctggc gtagacaatt ttttcctgag cattctggat
     4561 agcagtggtg acctggctaa gtacactctc ttccagtgac actttttgag tggcgaaagt
     4621 acgtgccagc gtgtactggc tgttttgcgc ctgtgcctgg gagagaacta cggcttgtga
     4681 tgctgcaatg ggatcgtcag aagggttaac gactcgctta cccgtcgaca tctgctcgcc
     4741 gtacttcatc cattctgcct gagaattggt gatgccgcgc atgttttgct ggtacatcat
     4801 ctgggtactg aaacgcattt tatctcccct tagcgaatgt taatcagcgc atcaaaaatc
     4861 gcgtttgccg tctgcagaac ctgcgcattc gccaggtaat actgctgaaa acgttgcaga
     4921 tttccgtact cctcatcgag attgacaccg gaaatcgact gctgctgatt ggaaagctgc
     4981 gtcaccacat taccttgcgt ggtgctactg gttttcaacg tcgcggtttt attaccgata
     5041 tcactcacta acgaggcata agcgtcgtta aaggatttcg caccgcccac cgttttactg
     5101 ttgctttgca gatccagcag ggcctgaccg ttgcggttgt cgctatcacc cgcatcttct
     5161 tcgctcgcca tcgcgatttt cgcttcgtcg gtgattaata catccatgtt gacgatggcg
     5221 tcacttactg gtttcagcgt gaagctgtcg ttaacggcag gcgttcctgt aaacgtcaac
     5281 tccagaccat caaatgccac tttaccgttg gcatccggcg tcaccgtaaa agtggtattg
     5341 ctggcaaggc gggtgacctg ccactgatta ttatcgaacg agattttgta atctgtcgcc
     5401 agtaccgcgg aggcatcagt taccgtggca ccgatcgcaa cgtcaccttt gtttttcgtg
     5461 ttttgcagaa ccgcgggctt accgatagca aagaaatctt caccagcatc gccgttggca
     5521 tcaaatccgg ctttgtgttg gctgttgaaa gcctcggcaa atgccagcgc cagttgtcca
     5581 agcgtattac gcgtctggtc cagatcctga gaacggaatg tcagaatgcc gcccagcgac
     5641 ccggtattca gtaatttctc cgggatctca atattgcctg ccgtcccatc aacgtaagcg
     5701 acagtcgtac gagaagggtc ggcgctggaa ggaactgccg ccagttgccg cgccgtactt
     5761 ccctgaacca gtgagtaacc attggccatc gtgatgttat aagtaccgcc atcctgaacg
     5821 ctgacttcta cgccaacaat ctggtttaat tcgctcacca gttgatcgcg ttgatccagc
     5881 agattgttag gtgacgcccc tgcccccacg cctgtcaggc gcgagatttg atcgttcagg
     5941 ctggcaattt gtttagcgta attgttgatc tgatcaacgc tggcacctat cgcgatattg
     6001 acctgtttgt cctggtcgcg cagatattga tcggtggttt taaactgatt caccaaccct
     6061 tctgatttcc caatcagcgc ctggcgcgct gccgggtctt ccgcgttact caccagcgtt
     6121 tgcaggctgg tgaagaaatc ctgcatctgt gttgccagcg aagaggtact ggtggagagc
     6181 atattgtcga ttttcgacat ctgctcatag cgggcggtca ggccgctact ttgcgtctgc
     6241 gcagcacgta actggttggt aataaacgca tcatactcac gctgcacacc agaaacgtag
     6301 acgccattgc caacccagcc gccagcgccc aacgtgctat tggcctgcgc cataatagtg
     6361 gtttggcggg tatatccggc aacgttatag ctggagatat tattacttgc cgtatttaac
     6421 gccgcctggg ccgcgttcag tccgctcatg gcgttattga tcaagctgga catggaggtt
     6481 ccttataagc cttcaattac agagtattat cggcagcgac ccgccggact tgagttattc
     6541 agaacagatt atcaatgttc atgctgtagg ttttgctcac cttgtcgctt atcgatttca
     6601 tctgctgaat catgttggtg agtttgcggg catagtgagg atcggtggca tagcccgcgt
     6661 cctgtagggc ctgcgccccc tgttccgcac tcgcggcggt cgtcacggcg gcgtagcgcg
     6721 ggttacgcgt taacagccca acgtaatccg acaaggcttc cagatacgag ctgtagacgc
     6781 gaaacttcgc ttttactttc ttcgcttcgc cgttttcata ttcagtcgtg gtgatttcag
     6841 taactggccc tttccagttg ccagaggctt tgacaccaaa caggttatag ctcggctcgc
     6901 cgttttcgcg gcggatttgc cgttgccccc aaccagattc cagtgccgcc tgagcgagga
     6961 tcaaatgatg tggcacaccg ctttgctggc ttgccagttg ggcgggcagc gagagttgcg
     7021 cgaggaatgc tttactgtca cccggcagcg aatcatcgta gttacgtggc acggcctttt
     7081 gcaccagctg cgaaagcgcc tgattttgat aacgcaccac ggtttcgagc gggaatttca
     7141 tcggtgctgc tggcgtggac tcctctggca atggttgttc tggcgtcatc tgtttaacca
     7201 tcatctctgc aagccccaga cctttgcccg ccgtcatctg ttgggcaatc tgctggtcat
     7261 acatactggt atacagacga gtgtgctcgc tgctgaacag gccatctttt ggtaaagcgt
     7321 cgcgcatgct tttcaacatc atctgcacga acatcccttc cacctggcgg gccaccggac
     7381 ggatatttgc cgccggatct tcgcccgctt tcgcctttag ttcgttgagt gattgcgcat
     7441 cccaggccgc acttgccagt agtttgctgt cgctgatcat cagatgattt ccagttttgc
     7501 ccgcagacat cccgcacttt gcattgattg caaaatagac atcagatcca tcggcgtagc
     7561 gcccagcgca ttgagcgcgc gcaccacgtt attgaggctg gcgctggaac gtacgctttg
     7621 cagcgaaccg ccgctctggc gtaaatcgat ctgcgtttgt ggagtaacca cagtctgtcc
     7681 accaccaaac ggtgtatctg gctggctgac attggcctga cgattgactg ttactgagag
     7741 atttccctgc gctaccgcgc agctgtcgag ggtcacttcg cgattcatca ccaccgaacc
     7801 ggtgcgcgag ttaatcacta ctttagcgtc ctgcggggtg acattaacct gcatattctg
     7861 gatatctgca aggaaacgga cctgggaact gttgccactt ggtacgcgca cctgaatagt
     7921 ccgggcatct aacgcggtgg cgctgccata tccacgcacg cggttgatgg tgtcagcgat
     7981 ttgctgcgcc atgctgaaat cttcgtcgtt aagttgcaaa ttaagggtat tcccgacgcc
     8041 aaactggctg ggcaattcac gttcaataac cgcaccattg gtgatccgtc caccgttcag
     8101 ttggttcacc tgaacactgc taccgccagc ggaggctcct gcgccgccaa ccagaatatt
     8161 gccctgcgcc agcgcataca cctgactgtc aacgccctta agcggcgtca tcaacaacgt
     8221 acctccacgc aagcttttgg catttcccat ggaagaaacc accacatcga tggtttgccc
     8281 ctgacgtcca aacggaggaa gtgacgctgt caccattacc gcagcgacgt tttttagctg
     8341 catattggtg cccgtcggaa cggtaattcc cagctgtgag agcatgttat taagcgtttg
     8401 tgtggtaaac ggcgtctggg ttgtctggtc accggtgcca tccagcccca ccaccagacc
     8461 atagccaatc agtgagtttt gccttacccc ctgaacactg gtgagatcgc gaatacgctc
     8521 agcctgagcc gccgtcgtaa ccagtagaag aattaatgca gagagaaatt taatcaccac
     8581 agcctcactt acattggcga caggttaagg aagaaacgct gcaaccagcc catattttgc
     8641 gcttcgttaa tgtagccatt gcctacgtat tcaatgcgcg catccgccac ctgagtagac
     8701 ggtacggtat tgctgccgct gatagtgcgt ggattaacca cgccagagaa gcgaataaat
     8761 tcggtaccct gattaatggc aatctgtttt tcacccacca catgcaggtt gccgttgacc
     8821 agtacctggt caaccgtcac cgtcaacgtg ccgctaaagg tattgctggc attggccccg
     8881 ccctttccgt tgaacgtgtt accaccggag gcttcgacat cggcacgagc gttaccaaac
     8941 agcccctgca aatagcgcgg cacagtatca aagccaaaat tagttttacc gtcacggctg
     9001 gcattcgcag aggagctttt gctggcgctg acgttctcct gcaacacgat ggtcagcgta
     9061 tcgccaatat tgcgtggtcg acgatcttca aacagcggtt gatagccata gttaatcggc
     9121 tgagcagact ggaaaataga accgttggcg acgggcgtcg gaccgggaac cggttgtgca
     9181 ctggtcgccc cctgcaccag cggcgtggag ggtatccagg cgcagccggt tagtgaaagc
     9241 accaacaagc tggaaatggc ataagtatgc gcagcgtttt tttgcatggc tatcatcttc
     9301 aaaaatcagt aaaccggtga acctgccacc ggttaagcct tagagttgcg tcagtttttg
     9361 cagcatctga tcggtggtgg acaccgcttt actgttgatt tcgtaagcgc gttgcacctg
     9421 aatcatattg accagttctt ccgccacgtt gacgttagac gtttcaacat acccttgata
     9481 cagcagtccc gcgccgttca ggcccggcgt gctttcgttc ggtgcaccag aggattgcgt
     9541 ttcggtgtag aggttttcgc caatgctctc cagcccggtg tcattcataa aggtggtgag
     9601 attgagctgc ccaacctgaa ccggagctgc ctggccttgt tgggttacgc tgaccacgcc
     9661 atcacgaccg atggtgatac ttaacgcatt cgccggaatg gtgatcgctg gctgcacctg
     9721 aaaaccacca gccgtcacca gctgcccgtt ctgatccacc tggaaagagc cgtcacgggt
     9781 ataggctgat gaaccatctg gcaacatcac ctggaaaaag ccctgccctt taatcgcgac
     9841 atctttgctg ttgttggtct gcgacaggtt tccctggctg tgtaagcgtt cagtggcgac
     9901 cgggcgtacc cccgtgccga tttgtaatcc ggagggtaag gtggtttgtt cggaagactg
     9961 tgcccccggc tggcgaatgg tttgataaag cagatcttca aacaccgcgc gctgacgctt
    10021 aaaaccgtta gtactgacgt ttgccaggtt gttggcaatg acgtccatat tggtttgctg
    10081 ggcgtcaagg cccgttttgg cgatccataa tgaactgatc ataaaatgtc ctgtagtgga
    10141 ttagtgacaa gcagagcaga agcacgcgac agtgtgaatt tcccctcacc ctaaccctct
    10201 ccccaaaggg gcgagggaat agtccgcgcg cttttattgc aatgtcatca atcgttcaat
    10261 taactcatcg acagcagttg gttggcacgg cctgcgttat catcgacgct gctgatcacc
    10321 ttcatctgca tttcaaaacg ccgcgcgctg gcaatcatgt cgctcattgc cgcaacggca
    10381 ttgacgttac tgccttccag aacccccgac atcacacgca aggttggatc tgcctgcagt
    10441 atcggcccac gcgtggcctg gctttctgcg cttaaacgaa aaatgccgtc gtcaccgcgt
    10501 tgcacttcgc tgcccgtggc tttcaccagt ttcagacgcc ccactggcgc aaccgtattt
    10561 gccggatcgc ccggattgag cgccgagatt gtgccatcgg cagcaatagt gatttccgcc
    10621 ccttccggca cagcaattgg cccagcctcg cctatcaccg gatgcccctg aattgtcagt
    10681 tgcccggtgg gatcaacctg aatgctgcca ttacgcgtat acccttcgct gccgtcagcg
    10741 gactgcacgg ccagccagcc atcctgctgc aatgcaacgt ccagcgggcg cgaggtgtaa
    10801 tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg ccgttaccag cgtgcgcgtg
    10861 ggcagtgaaa gcccttccac cggcactgca cgcaaggcgt ttaactgcgc gcgaaaaccg
    10921 ggcgtagagg cattggccag attactggcg gttaccgcct gttgattcag cgtctggctg
    10981 gctgctccca tcgcggtata aattgcgtga tccattgagc tatcccgtca gcgattagcg
    11041 taagttaacc agcgtgttga ggatctggtc ctgggttttg atggtctggg cgttagactg
    11101 atagttacgc tgggcaacga tcatattgac cagttcttta ctgagatcga cgttggacgc
    11161 ttccaacgca ccgttggtca gggtgccaaa gttgcccgtc ccggctgtcc ccaacagcgc
    11221 cacgccagaa gattgcgtcg cagaccagac gttgtcgcct tcgaatgcca gaccttcgtt
    11281 gttggcaaag ttcgccagta caatctgccc cagcagttgt gtttgttcgt tggaatagtt
    11341 gccgacaacc gtaccgtcat cattgatttg ataactcacc agatcgcccg gtttgtagcc
    11401 attctgggtg gttgccacaa tattgttagc gccggtattt tgctgcatgg agttgaggaa
    11461 gctcaggcta aacgtggcgg gatctgcgcc gttaattgcg ccagtggtaa tattctctgt
    11521 tggatttgag gtcagaacgc cattggcatt aaacaccagc ttcattgcag gctcggctgt
    11581 acctgttgga tcactgctat cctgggtgta gacctgccag ttattatccc cggtcttcac
    11641 aaagtagacg ctcatgtcat gagcattacc ctgactgtcg aaaacagtca ccgaaccttt
    11701 tttgttatag ctatccgcat tgctggcgct gaatggcgta acagtaggaa gcggatcact
    11761 ggaattcagg ttgatctgca tcgatgccgt ggtggtagtt ttcgctgcca tcagggtatt
    11821 cgggatcgaa atattggtcg gattcgcccc ttgctgaata gtcggcggcg taccggttgc
    11881 cgggtaaccc gtcagctgta aaccttgcat attcaccagg ttacggtttt catccagctt
    11941 aaattgtccg ttacggctgt agaacaccga accgttgctg tctaccagac ggaaaaaacc
    12001 gttctggctg atagcaacgt ccagacctcg cccggtgttg gtggtcgtgc catcggtaaa
    12061 gtcctgagtg ataccggcaa cttttacccc cagtcccact ttcgaaccgg caaacatatc
    12121 ggcaaaagag gccgtgcctg atttaaagcc gtaggtggcg gagttggcga tattgttgcc
    12181 aataacatcg aggttggtgg cggcagcgtt taatccgcta accgcttgag aaaaggccat
    12241 gactgactcc tgaaatgtga aggcttaaat tatctgccgt acttcgtcga gggtggtggt
    12301 gccgtaagtg ccgagatcca gcgtattacc gctgttgccg cggatcacac cctgcaccag
    12361 agcaaactgc agcggctggg caaccagttg tgtaccaccg ttactggcgc taatcgctac
    12421 attgtaagaa ccgttcggcg cagtgctgcc atcagtcaac gtaccgtccc aggtgaaact
    12481 gtgaactcca gcggtcagtt caccaatatc aatggtgcgc acaaccgcgc catttttatc
    12541 ggtgatggtg gccgtaactt tgtctgccgc ctgttgcagc tcaacaccaa acggcgtggt
    12601 cgtggtcaca gccccttctt cactgccggt tcccgcaaga acagttgtgc cggggatcat
    12661 cacgccgtga ccgatcaggt tactggcctg taacgactgg ctgttatcaa tctgtccgga
    12721 aatagatccg agtgtggtat tgagtttttc aatcccgctg accgtgctga tttgtgccaa
    12781 ttgcgacgtc agctcgttgt tttccattgg attggtcggg tcctggtttt tcagctgcgc
    12841 caccagcaaa gtcagaaaac tgctttgtaa atctgcggcg ttgctgcccg tgagcgaact
    12901 actgctggtg gtactgacgc cggtatttgt cggatcggtg gtggttaccg caatggacat
    12961 agctttctcc tttattgacc gagcgtaagg gttttcagca tcatgctttt caccgtgttg
    13021 agcacttcaa cgttggcctg atagctgcgt gacgccgaca tggtgttaac catctccccg
    13081 acgacatcaa cgttcggcat ttttacgtag ccctttgcat ctgccagcgg attacccggt
    13141 tcataaacca gtttgtccgg ggcctgactt tctataacat cggcaacctt tacgccgcct
    13201 gtcgcagcac ctggtgcagc gttaacctgg aataccacct gttttgcccg atatggctgt
    13261 ccatcaggac cggtcacgct atcagcattt gccagattac tggccgccac gttcaggcgc
    13321 tgggactggg cagttaacgc cgacccggcg atatcaaaaa tattcagcag tgccatccgt
    13381 taatttccgc tctgtaaaac gttcatcatg cctttgattt gcccgctcaa cgcgctaagg
    13441 ctcatctggt attgcaggct gttatcggca aactgggtgc gttcgcgatc catatcgacg
    13501 gtattaccgt caagcgaagg ctggtccgga atacggtatt gcagttctgc ggtaggaggc
    13561 gtcagcgcct gcgccgggat gtgttgcgtt gaggtcatcg tcagtgcaac cacactggtt
    13621 gcatcccgtc cacgttgcat gactttttta agttcactgg caaaatcgat atcgcgcgcc
    13681 tgataaccag gggtatcggc attggcgatg tttgctgcca gcacttcctg acgctgggcg
    13741 cgcagattga gcgcctcttg ttgaaaacgt aaggcggcgt cgagcttatc gagcatatct
    13801 cctccgcagg tatcaaaatt ctgccatcag cttaaatgcc tttacgcgcg cgttatcggc
    13861 ggaataaacg caaaatgggt cgctatttat gccgttgatg gtcattgcgg acaggtacaa
    13921 ttcacgttgt agaaatggct gggggcgaaa atgctgacaa taaaacgtag cgtggcgatc
    13981 atcgcgatac tgttcagtcc gttaagtacg gcgagcaatc tcacatcgca attgcacaac
    14041 ttttttagcg cccaactcgc gggggtaagt gatgaggttc gtgtttctat tcgtacagcg
    14101 cccaatctac taccgccatg cgagcagcca ttgctttcga tgagcaataa ttcccgcctg
    14161 tggggcaatg tgaatgtgtt ggcacgctgc ggtaacgaca aacgatattt acaggttaat
    14221 gtgcaggcca caggaaatta tgtggttgcc gcgatgccca ttgtgcgggg aggaaagctg
    14281 gaagctggca atgtcaaact aaaacgcgga cggctggata ccctgccacc gcgtacggtg
    14341 ctggatatca atcaacttgt tgatgccatt agcctgcgcg atctatcacc cgatcaacct
    14401 atccagttaa cccagtttcg ccaggcatgg cgggtaaaag cgggacaacg cgtcaatgtg
    14461 atcgccagcg gtgatgggtt tagcgccaac gcagaaggtc aggcgctgaa caatgcagcc
    14521 gtcgcacaga atgcgcgggt gcgcatggta tcgggacagg tagtcagcgg cgttgttgat
    14581 gcagatggga atattcttat aaacctgtaa gctgttaaag attacccgtc ccttgccgat
    14641 aaataagcaa cacatgataa aagcgccctc aatgaggaat aaaccatgag tattgatcgc
    14701 acttcgcctc tgaagcctgt aagcaccgtt caaccgcgcg aaaccactga cgcgccggta
    14761 acgaacagcc gggcggcaaa aacaaccgcc tccaccagca ccagtgtgac gttaagcgac
    14821 gcgcaagcaa aactgatgca acccggcagc agtgatatca atcttgaacg tgtcgaagcg
    14881 ttaaaactgg cgattcgtaa cggtgaacta aaaatggaca ccggcaaaat tgccgatgcg
    14941 ctgatcaacg aagcgcagca agacttgcag agtaactgat cgtatgacac gtcttgcaga
    15001 gatcctcgac cagatgtccg ctgtgcttaa cgatctcaaa acggtaatgg atcaagagca
    15061 gcaacatctc tctatggggc agatcaacgg cagccagttg caatggatta cagaacaaaa
    15121 aagctcactg ctggcgacgc tggattacct cgaacagtta cgcaagaaag aacccaatac
    15181 agcaaatagc gttgatatta gtcaacgctg gcaggaaatt actgtgaaaa cgcagcaact
    15241 acgccaaatg aatcaacata acggctggtt actggaagga cagattgagc gcaatcaaca
    15301 ggcgctggaa atgttgaaac cgcatcagga accgacgcta tatggggcga acggtcagac
    15361 ctcaacaacc catcgcggcg gtaaaaagat ttcgatctga atattatctc cggcctgcac
    15421 tgcaggccgg aatgcattgt tacaccgtcc ggcgggcaaa ttctttaact ttgaagccca
    15481 gtaccgccag tgcagcgaag tacgcggcaa tccccgccag cacgaccgcc attaaacgca
    15541 gtaagcgcca gggcatggta cccaatgacc actccggcat gatatgtaac atacctaaaa
    15601 gcacgccaga catcaccagt accgccacca ccagacgcaa cagaaacgcc atccagccgg
    15661 gttgcggagt aaagattttc tgcttacgca actgccagta aagcagcgaa gcattcagac
    15721 acgccgccag accaatagaa agtgacagcc cggcatgttt caacggacca ataaacgcca
    15781 ggttcatcaa ttgcgttaaa atcagcgtaa cgatggcaat tttcactggc gttttaatgt
    15841 cctggcggga ataaaagcca ggagccaaca ctttcactac aatcaggcca atcaaaccca
    15901 ccgagtaggc aattaacgcc cgctgggtca tcagcgcatc aaacgcggta aatttaccgt
    15961 actggaacag cgaaacggtt aacggacctg aaagaatgcc caacgcaacc gcactcggca
    16021 gcgccaacag gaaacaaaga cgcaaccccc agtccatcaa acggttgtat tcatcatgat
    16081 tgccactggc aaaacttttc gacagcgacg gcagcaaaat ggtgccaagc gccacgccca
    16141 gcacaccgga cggaaactcc attaagcggt cggcgtaata catccaagac accgaaccgg
    16201 aagcaagaaa cgaggcaaaa atggtgttga tgattaagga gatctggctc acagagacgc
    16261 caaggatcgc cggtcccatc tgtttcacca cgcgcattgc tccggcatcg tggaagttaa
    16321 tgcgcggcag gaccagcatg ccgatcttct tcaggtgcgg tagctgatac accagctgca
    16381 ggatgccgcc gaccgtaaca gcccacgcca gcgccagcac cggtgggtta aagtacggtg
    16441 cggcaaacag cgcgaaacca atcatgctga tgttaagcag tgttggagca aacgccggaa
    16501 tcgagaagcg gttccacgta ttcagaatcg ctcccaccag cgacgccagg gagatcagca
    16561 agatataggg aaaggtaatc tttagtagct ggctggtcag ggcaaatttg tcagctgtgt
    16621 cagcgaagcc tggcgcggtc accatgatca cccacggtgc ggcgagcatg ccagcgaccg
    16681 tcacaaccgc cagcgcgagt gtcagcaggc cagaaacata agagacaaag acccgcgtgg
    16741 cgtcttcacc ctgcttactt ttatattccg ccagaatcgg tacaaatgcc tgggaaaatg
    16801 ccccttcggc aaagatacgg cgtaacaagt taggaagttt aaaagcgacg aaaaaggcgt
    16861 cggttgccat ccctgcgcca aagattctgg cgacaattgc gtctcgtgcg aagccaagca
    16921 cacgcgaaaa catggtcatc gagctgacgg cggccagcga ttttaataaa ttcatcggtg
    16981 ttctaatcca gacccacggg caaaacgcct gcaaggcagg cgctgaaaga ggcgaatagt
    17041 ctacctggat tatggtgaat tgctaccgcc agatgttaca gggtttattc actcatcgca
    17101 tcgcgccaga tcttgtcaac gatacgttgc gccagcacgg cctgttcgcc ggcggtttgc
    17161 ggaactgtct gattttgcac acattcaatg aagtgccgcg cacagccgac aaatccacgt
    17221 tgttcaagag tgctctgcca accaggaatc ggtttatgca ctacgccctg cccgcgctcc
    17281 tcacgccatt cgcgcatatc cgtaatgtcg atgagcgcac cgtcagtcac ggcctgcacg
    17341 gtttcacgct gacttccggc acggcgatgc atacaggtgg tgatctgcaa aggaccggcc
    17401 gaaaaatggt gctcggcaaa cagcatttcg ccagcgtcgt tagtcagtag cgtaccgcca
    17461 tccagagagg ctttgccgcc cgacaaccac agcgcggtat ccaccacatg cagataatca
    17521 tccagcaacg tgaaataaag atcgtgtggc ccgacactat tgctacgatg tttatccatt
    17581 cttagcgaag ctgcggtagc gagttgcgtt tttaactcac cgtagagtgg tgcgaaacga
    17641 cggttaaaac cgaccatcaa cgtcagtttt ttccgcgccg ccagttccac cagccgttca
    17701 gcatcgcgca gattttctgc cagcggttta tcgacacaga catgtacacc cgcattgagt
    17761 aacgtactga ccacgtcaaa gtggctggcg gtgctggaat gcacaaaaac cgcatcgcaa
    17821 ctggcggcaa ggctggataa cgaatcggca taaggaatgc gccagctttc acaaattggc
    17881 agggctttcg cgcgcgtagg cgaccaggct ccttgtaaca tccagtcaga cgctgccgcc
    17941 agcaccggta accacgcttt ttgcgcaatg ccgcctaatc ccacaacgcc gatacgtaat
    18001 tttttcactt taatctccca gatgggccag caacgaatca agacgctgtt tcagttctgc
    18061 cacttcgatt tccagggctt cgacgcgggc ctgtaaatca ccatcaaccg cgttcgacat
    18121 atccgttacc gccggctgat cctctacctc accactgaaa agatgcatgt agcggctttc
    18181 gcgtttaccc ggttcgcggg ccagacgcac cacaaaagga ccatcttcgc gatttgccag
    18241 ttgttccagc gtcgactcca cttccgccat atcgctgaat tcatacattc gcgcggcgcg
    18301 gctgcgcagt tcacctggcg tctgggcacc acgcaataac aacgtggtga ttaacgccac
    18361 ttccgctgcg ctcagtttca gatcgccaaa ttctgaatta caaaaacgtt gctcatattt
    18421 ggtgacccga ttaccaaaac cgctcactgt gcgtagataa tgacgtttga ccagattatc
    18481 cagctgttcc tgcacttcgg attcactcag attcatgacc ggttcacggt tcgttttctg
    18541 attacaggcc gtgactacac cattgactga gagcgggtat tgctccggcg tggtcacctg
    18601 cttttccagc aagcaaccaa tcacgcgagc ttcaagggca gtaagttgat atttcatgag
    18661 attctcctta gcggccgggc gtccagtctg gggtagttaa tgccgtcagt acgtgatcgc
    18721 gccattgtcc atcaatcaac agatagtctt tcgcatagcc ttctttttca aaacccagtc
    18781 gcgccagtaa atcaccgctg cgtttattgt gcggcatata attagccata atgcgatgaa
    18841 tatgttgggt gcgctgcata taacgaatgg ctgcggtcag ggcttcaaac atgagtcctt
    18901 tgccctgcca tttttgccca atcgaataac cgagatagca ggcatgaaaa gagccacgaa
    18961 caacattgga aaaattggca acgccaataa tctctttttc gtccgggtcg aataagccaa
    19021 agtagaaagc tgaaccttgt ttatgaaatt cgttaatcat ccccagcctg gcctgccagc
    19081 ctgatggata acagtggctt tcgtcgcgca ctggctccca gggcttgagg aaatggcgat
    19141 tctctgcgta ataatccgca agacgccagg catcacgatc atgcaccaga cgcacgacca
    19201 gtcggtctgt ggttaagcgc acttttggca cgttactgcg atagccaaac attctatacc
    19261 tactccttcc cgtaacgtct ttaatacgct gtaagtatta ctatacctgc gcaaccagac
    19321 cctgtgaaaa gtccaaaatg ccattttctg cattattcac atatttgata aaaactctct
    19381 atcaaagcga cgttttgata aaaaaatatt gtcccctaag tgatgggaaa agtcttagcg
    19441 gcaatgcaga atagaaagtg ctcatctttt atttcccggg aggggaaatg tcccgcgtat
    19501 cgcaggcgag gaacctgggt aaatatttcc tgctcatcga taatatgctg gtcgtgctgg
    19561 ggttctttgt tgtcttcccg ctgatctcta tccgcttcgt tgatcaaatg ggctgggccg
    19621 ccgtcatggt cggtattgct ctcggtctac gccagtttat tcagcaaggt ctgggtattt
    19681 tcggcggcgc aattgccgac cgctttggtg ccaaaccgat gattgttacc ggtatgctga
    19741 tgcgcgccgc cggattcgcc acaatgggta tcgcccacga accgtggcta ttgtggtttt
    19801 catgcctgct ctcgggactc ggcggcacgt tgtttgatcc gccgcgttcg gcgctggtgg
    19861 tgaaactaat ccgtccacaa caacgtggtc gttttttctc gctgttgatg atgcaggaca
    19921 gtgccggtgc ggtcattggc gcattgttgg ggagctggct gttgcaatac gactttcgcc
    19981 tggtctgcgc cacaggggcc gttttgtttg tgctgtgtgc ggcgttcaat gcgtggttgt
    20041 taccggcatg gaaactctcc accgtacgca cgcccgttcg cgaaggcatg acccgcgtga
    20101 tgcgtgacaa gcgttttgtc acctatgtcc tgacgctggc gggttactac atgctggctg
    20161 tacaagtgat gctgatgctg ccaattatgg tcaacgacgt ggctggcgca ccctctgccg
    20221 ttaaatggat gtatgccatt gaagcgtgtc tgtcgttaac gttgctctac cctatcgccc
    20281 gctggagtga aaagcatttt cgtctggaac accggttgat ggctgggctg ttgataatgt
    20341 cattaagcat gatgccggtg ggcatggtca gcggcctgca acaacttttc accctgattt
    20401 gtctgtttta tatcgggtcg atcattgccg agcctgcgcg tgaaacctta agtgcttcgc
    20461 tggcggacgc aagagctcgc ggcagctata tggggtttag ccgtctgggt ctggcgattg
    20521 gcggcgctat tggttatatc ggtggtggct ggctgtttga cctgggcaaa tcggcgcacc
    20581 agccagagct tccgtggatg atgctgggaa ttattggcat cttcactttc cttgcgctgg
    20641 gttggcagtt tagtcagaaa cgcgccgcgc gtcgtttgct tgaacgcgac gcctgatttg
    20701 atactccccc ctgactcctc catactgaaa gtgaactgac agccaaatgg aggagtcacg
    20761 tgaagctata catttacgat cactgccctt actgcctcaa agcccgcatg attttcggcc
    20821 tgaaaaatat ccccgtcgaa ttacatgttc tgctcaacga cgacgcagaa acacccaccc
    20881 ggatggtcgg tcaaaaacag gttcccattc tgcaaaaaga tgacagccgc tatatgccag
    20941 aaagcatgga catcgttcac tatgtcgata aactcgacgg caaaccgtta ctgaccggca
    21001 aacgttcccc tgccattgaa gagtggctgc gcaaggtcaa tggctacgca aacaaactgc
    21061 tgttgccgcg ttttgccaaa tcggcattcg atgagttttc tactcccgcc gcgcgcaaat
    21121 atttcgtcga caagaaagag gccagcgcgg gtaattttgc cgacctgctg gcccactctg
    21181 acggtctgat taagaatatc agcgatgatt tacgtgcgct ggacaaactg atcgtcaaac
    21241 cgaacgccgt gaatggcgaa ctttcggaag atgatattca gctatttccg ctgctgcgta
    21301 atctgacgct ggtagccgga atcaactggc caagccgcgt tgctgattac cgcgataata
    21361 tggcgaaaca gacacaaatc aatttgttat catcaatggc gatttaatcc tgacccgccg
    21421 cggcagcctg tattcgcggg ttgccgccgt tttcagtttt ttctgctggc gagtttatcg
    21481 ctggtgacac atgctgcgtt gtgtcaggat attgacgcag atttcatgtc gaggactacc
    21541 atgaacaagt ttttatttgc cgctgcattg atcgtcagtg gcctgctcgt tggctgtaat
    21601 caactcaccc aatacaccat caccgaacaa gaaattaacc agtcgcttgc gaaacataat
    21661 aatttctcaa aagatatcgg tttacccggc gtggctgacg cccatattgt tctgacaaac
    21721 ttgaccagcc aaattggtcg cgaagagccg aataaggtta ccctaaccgg agacgccaat
    21781 ctggacatga actccctgtt cggtagtcag aaagcgacca tgaaactgaa gctgaaagcg
    21841 ctgcccgtgt ttgataaaga aaaaggtgcg atcttcctga aagagatgga agtggtcgat
    21901 gcgacggtac aaccggaaaa aatgcaaacg gtgatgcaaa cgttgcttcc ctatttgaac
    21961 caggcattac gcaattactt taaccagcaa cctgcttacg tcctgcgcga agatggcagt
    22021 cagggcgaag caatggcgaa aaaactggcg aaaggcattg aagtgaagcc aggcgaaatt
    22081 gtcattccgt ttacggatta atgacgaggg cgcattcgcg ccctttattt ttcgtgcaaa
    22141 ggaaaacgtt tccgcttatc ctttgtgtcc ggcaaaaaca tcccttcagc cggagcatag
    22201 agattaatga ctgcaccatc ccaggtatta aagatccgcc gcccagacga ctggcacctt
    22261 cacctccgcg atggcgacat gttaaaaact gtcgtgccgt ataccagcga aatttatgga
    22321 cgggctatcg taatgcccaa tctggctccg cccgtgacca cagttgaggc tgccgtggcg
    22381 tatcgccaac gtattcttga tgccgtacct gccgggcaca atttcacccc attgatgacc
    22441 tgttatttaa cagattcgct ggatcctaat gagctggagc gcggatttaa cgaaggcgtg
    22501 ttcaccgctg caaaacttta tccggcaaac gcaaccacta actccagcca cggcgtgacg
    22561 tcaattgacg caatcatgcc ggtacttgag cgcatggaaa aaatcggtat gccgctactg
    22621 gtgcatggtg aagtgacaca tgcagatatc gacatttttg atcgtgaagc gcgctttata
    22681 gaaagcgtga tggaacctct gcgccagcgc ctgactgcgc tgaaagtcgt ttttgagcac
    22741 atcaccacca aagatgctgc cgactatgtc cgtgacggaa atgaacggct ggctgccacc
    22801 atcactccgc agcatctgat gtttaaccgc aaccatatgc tggttggtgg cgtgcgtccg
    22861 cacctgtatt gtttacccat cctcaaacgc aatattcacc aacaggcatt gcgtgaactg
    22921 gtcgccagcg gttttaatcg agtattcctc ggtacggatt ctgcgccaca tgcacgtcat
    22981 cgcaaagaga gcagttgcgg ctgcgcgggc tgcttcaacg ccccaaccgc gctgggcagt
    23041 tacgctaccg tctttgaaga gatgaatgct ctgcagcact ttgaagcatt ctgttctgta
    23101 aacggcccgc agttctatgg cttgccggtc aacgacacat tcatcgaact ggtacgtgaa
    23161 gagcaacagg ttgctgaaag catcgcactg actgatgaca ccctggtgcc attcctcgcc
    23221 ggggaaacgg tacgctggtc cgttaaacaa taaaaattgt agcttcctgt tgtcattagg
    23281 ttattttacc tgtataaata accagtatat tcaacagggg gctattatgc gaattgaagt
    23341 caccatagcg aaaacttctc cattgccagc tggggctatt gacgccctgg ctggcgaact
    23401 ttcccgccgt attcagtatg cgtttcctga taatgaaggc cacgtatcgg tacgttatgc
    23461 cgcagcgaat aatttatcgg ttattggcgc aacaaaagaa gataaacagc gcattagcga
    23521 aattctccag gaaacgtggg aaagcgccga tgactggttt gtcagcgaat aatatgcagt
    23581 gatttttgtt tttgccgggt cgccccggct tttttatttt acttacaaat tttgagtaca
    23641 aaatttctac acttcctcaa aataattgca caatatgcta aaaaaatatg aatgacatgc
    23701 tgttttattg ttcatataaa tcttaaaagc agaaatttat ctgttccccc ctttaaaaat
    23761 tcggtgaata cccttactta ttggtatact gaagttgctt cagataaaaa cgcattgaac
    23821 ctcgaataac gttgtctagt aacacgaatt agggggccat gatggaaaaa aataatgaag
    23881 tcattcagac tcatccgctc gtagggtggg acatcagcac cgttgatagc tatgatgcgc
    23941 tgatgttgcg tttgcactac cagaccccaa ataagtccga gcaggaaggg actgaagttg
    24001 gtcagacgct ctggttaacc actgatgttg ccagacaatt tatttcgata ttagaagcag
    24061 gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga gtatcggcgt cattaacatg
    24121 acatgaccat ccacccaaaa ggcacctcac cggtgccttt accatttctg ggttgtatat
    24181 ctgcattcag ttaattactc tgcttaaacc atgagagcag agagaaaaaa atgaaatacg
    24241 atctcatcat tattggcagc ggttccgtag gcgctgccgc cgggtattat gcaacccgcg
    24301 ccggtttaaa cgtgctaatg accgacgccc atatgccacc gcatcaacac ggcagccacc
    24361 acggcgatac gcgattaatt cgccatgctt atggtgaagg cgaaaagtat gtcccgttgg
    24421 tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca caacgaagat gatcccattt
    24481 ttgtacgctc tggtgtcatt aacctcggcc cggctgactc cgcatttctc gccaacgtcg
    24541 cccacagcgc cgaacagtgg caactcaacg ttgaaaagct cgatgcgcaa gggattatgg
    24601 cccgctggcc agaaatacgc gtcccggaca actacatcgg cttatttgag actgattccg
    24661 gttttttgcg cagcgaactg gcgattaaaa cctggatcca actggcgaag gaagcgggct
    24721 gtgcgcaact gttcaactgc ccggtcaccg caattcatca tgacgatgat ggcgtaacta
    24781 ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat tgtctgcgcg ggaacatggg
    24841 taaaagacct gctcccggag ctgcctgtcc agcccgtacg taaagtattt gcctggtatc
    24901 aggccgatgg ccgctatagc gtgaagaata aattcccggc gtttaccggt gaactgccca
    24961 atggcgatca atattatggt tttccggcag aaaacgacgc gttgaagatt ggcaaacata
    25021 acggaggcca ggttatccat tcagcggatg aacgtgttcc gtttgcggaa gtggtcagcg
    25081 atggttcgga agccttcccg ttcttgcgca atgtattgcc gggtatcggt tgctgcctgt
    25141 acggcgctgc ctgcacctat gataattcgc ctgacgaaga ttttattatc gataccctac
    25201 ccggccacga taatacactg ctcattaccg gcctgagtgg gcacggtttt aaatttgcgt
    25261 cagttttagg ggaaatagct gccgattttg cgcaagacaa aaaaagcgat tttgatttga
    25321 cgccattcag gctttcccgc ttccaataat catcattacg gcctccagaa tgaggccgtt
    25381 tttacaggag tatttaatgc gtcggctgtt gcactatctc atcaataata ttcgcgagca
    25441 tctgatgctt tatctttttc tctggggatt gttggcaatt atggatctga tctatgtgtt
    25501 ttatttttag ttttccgcac tgacataaac tgacaaatat ttaatctccc tttcatttag
    25561 cgtgaaaaat tgaatgagat tattcctgct tctacagcac tcaccagaca tctctgatgc
    25621 tgaacccact aaacttatga agattctttc attattaatt caaaattatt gaaataaaaa
    25681 taaatatcat tcaatttgtt atttttgtgt tgcaattcat tctccgacac gccattttct
    25741 ttctctcgca aattcgtatt ggatgcactt atgtcattca caaatacccc tgaacgctat
    25801 ggtgttatat ctgcggcctt ccattggcta agcgcaatta ttgtctacgg catgtttgct
    25861 ttaggtctgt ggatggtcac gctcagttat tacgatggct ggtatcacaa agcacccgaa
    25921 ctgcataaaa gcatcggtat tttgttaatg atggggctgg ttattcgcgt tctgtggcgt
    25981 gtcatatctc caccgccggg tccgctgccg agttattcgc ccatgactcg tcttgccgca
    26041 aaggctggac atctcgcatt gtacctattg ctgttcgcca tcggcatcag cggctatctg
    26101 atctcaactg ccgatggtaa accgatcagc gtttttggct ggtttgacgt ccccgcgacc
    26161 cttgccgacg ctggcgcaca ggcagacttt gccggtgccc tgcatttttg gctcgcgtgg
    26221 agcgtcgtag tactgtccgt tatgcacgga tttatggccc tgaagcatca tttcatcgat
    26281 aaagacgaca ctctgaagcg catgctggga aagtcgtcat ctgactatgg agtataaaaa
    26341 atgaaaaaaa gcctgcttgg tttaaccttc gcgtccctga tgttctctgc cggttcagcg
    26401 gttgccgccg attacaaaat tgacaaagaa ggtcagcacg cctttgttaa tttccgcatc
    26461 cagcaccttg gctatagctg gttatacggc acctttaaag atttcgacgg tacttttacc
    26521 tttgacgaaa aaaatccggc tgccgataaa gtgaatgtga caattaacac caccagcgtc
    26581 gatactaatc acgccgaacg cgataaacat cttcgcagtg cagatttcct caataccgca
    26641 aaatatccac aggcaacatt cacctccacc agcgtgaaga aagacggtga cgaactggat
    26701 attaccggcg atctgacgct gaatggcgta accaaacccg tcacgctgga agcgaaatta
    26761 attggtcagg gcgacgaccc atggggtggt aaacgtgcag gcttcgaggc cgaaggcaaa
    26821 attaagctca aggacttcaa tatcaagaca gatttaggtc cagcttctca ggaagtggat
    26881 ctgattattt cagtggaagg cgtacagcag aagtaagagt gaaatgttgc cggatggcac
    26941 gcatccggca atgatattta ttccgttgga tcaggaatgc ccagtgttgt attcagacgt
    27001 ccacgagact tattaaagat cttattgcca ttttcacgtc ctgcccgacg gcgtcgctgt
    27061 tcttctggcg gtaacgcgct ttcttcgcag caaatctcac tacaacaacc tttgtatttt
    27121 tccgcacata ctggacactg aataaacagc aggtggcagc catcattttt acagttggta
    27181 tggctgtcgc acggcgcgcc gcactggtgg caatgcgcga taatctcatc cgatatacgt
    27241 tcgcccatcc gctcgtcaaa aacaaaattt ttgccaataa aacgcaccgg caagccctgc
    27301 tcgcgcgcct tacgggcgta ttcaataatt ccgccctcga tatgccagac tttgttgaat
    27361 ccgttatgtt tcatccaggc actggccttt tcacaacgaa tgccgccggt gcagtacatg
    27421 acgatttttt tatctttatg tgcctgcatc atctcgactg cttttggcag ctgctcacgg
    27481 aaggtatctg ccggaatttc gagcgcgttt tcaaagtgcc ccacttcata ctcatagtgg
    27541 ttacgcatgt cgataaatag tgcatcggga tcgtcaagca tggcgttcac ttccgccgct
    27601 tgcagatact caccaacatt gctggcatca aagtgaggat cgtcaatacc gtcggcaaca
    27661 atgcgatcgc gtaccttcat gcgcagtacc cagaaagatt tcccgtcatc atccaacgcg
    27721 atattcaggc gtaaaccctc taaagccggg tcgaaggcat agagctgcgc gcgaaatgtt
    27781 tcaacattgc tcgcaggtac gctgatttgc gcgttaatgc cctcatgcgc cagatacact
    27841 cgcccaaaaa cattcagcgc ggtaaacagc tgatataaag cgtcacgggt cgccttagga
    27901 tcggcgatgt ggaaatactt gtaaaacgaa atggtggttc gcggttcgct ctcagccaac
    27961 attttggctt ttagcgcgtc gttggaaatg cggttgtgta acactggcat ggtgtacggt
    28021 tcctgcgaga tgggaaagta aaaatccgcg gcatgatata gcaattatcg ataattaaca
    28081 tccacacatt ttacgctaca tttgcgcatt aaaaattatt tgttatttac aagcgcggca
    28141 atttcgccca gtcttcagcc acaaattttg gttgcgggcg aaaaaatgcg acaatacata
    28201 caattgcccg aataggttga aaaacaggat tgatatgacg aatctaccca agttctccac
    28261 cgcactgctt catccgcgtt attggttaac ctggttgggt attggcgtac tttggttagt
    28321 cgtgcaattg ccctacccgg ttatctaccg cctcggttgt ggattaggaa aactggcgtt
    28381 acgttttatg aaacgacgcg caaaaattgt gcatcgcaac ctggaactgt gcttcccgga
    28441 aatgagcgaa caagaacgcc gtaaaatggt ggtgaagaat ttcgaatccg ttggcatggg
    28501 cctgatggaa accggcatgg cgtggttctg gccggaccgc cgaatcgccc gctggacgga
    28561 agtgatcggc atggaacaca ttcgtgacgt gcaggcgcaa aaacgcggca tcctgttagt
    28621 tggcatccat tttctgacac tggagctggg tgcgcggcag tttggtatgc aggaaccggg
    28681 gattggcgtt taccgcccga acgataatcc actgattgac tggctacaaa cctggggccg
    28741 tttgcgctca aataaatcga tgctcgaccg caaagattta aaaggcatga ttaaagccct
    28801 gaaaaaaggc gaagtggtct ggtacgcacc ggatcatgat tacggcccgc gctcaagcgt
    28861 tttcgtcccg ttatttgccg ttgagcaggc tgcgaccacg accggaacct ggatgctggc
    28921 acggatgtcc ggcgcatgtc tggtgccctt cgttccacgc cgtaagccag atggcaaagg
    28981 gtatcaattg attatgctgc cgccagagtg ttctccgcca ctggatgatg ccgaaactac
    29041 cgccgcgtgg atgaacaaag tggtcgaaaa atgcatcatg atggcaccag agcagtatat
    29101 gtggttacac cgtcgcttta aaacacgccc ggaaggcgtt ccttcacgct attaaatctc
    29161 ccctgccgga tgcttcagaa tggcatccgg cattaccaca gcaaatcccc ctgatttagc
    29221 gataaaagct ctctggattg cgccccctgg aagtcgggcg cataattagt gtgcttatct
    29281 tttcttctta tgttcaccgc gcctggcgca ccaacagcgg attgctatgt caccctgtga
    29341 aaatgacacc cctataaact ggaaacgaaa cctgatcgtc gcctggctag gctgttttct
    29401 taccggtgcc gccttcagtc tggtaatgcc cttcttaccc ctctacgttg agcagcttgg
    29461 cgttaccggt cactccgccc tgaatatgtg gtccggtatt gtcttcagca ttacattttt
    29521 attttcggcc atcgcctcac cgttttgggg tggactcgcc gaccgtaaag gccgaaaact
    29581 catgctatta cgctctgccc tcggcatggg catcgtgatg gtgttgatgg gactggcaca
    29641 aaatatctgg cagtttttga tcctacgggc gcttcttggg ttacttggcg gatttgtccc
    29701 caacgctaat gctcttatcg ccacacaagt accgcgtaat aaaagcggct gggcgctggg
    29761 tacgctctcc acaggcggcg ttagtggtgc gttgctcggc ccaatggctg gcggcctgct
    29821 cgccgatagc tacggcttac gtccggtatt ctttattacc gccagtgtgc tcatactctg
    29881 ctttttcgtc accctgttct gcatcagaga aaaattccag ccggtcagca aaaaagagat
    29941 gctgcacatg cgggaagtgg tgacatcact taaaaacccg aaactggtac tcagcctgtt
    30001 tgtcaccacg ttaatcatcc aggtggcgac aggctcaatt gcccccattc tgacgctgta
    30061 tgtccgcgaa ctggcgggta acgtcagtaa cgtcgccttt atcagtggca tgatcgcctc
    30121 ggtgccaggc gtggcggctc tgctaagcgc accacgactc ggcaaacttg gcgatcgaat
    30181 cggacccgaa aagatcctga ttacagcgct gatcttttct gtactgctgt tgatcccaat
    30241 gtcttacgtt cagacgccat tgcaacttgg gattttacgt tttttgctcg gtgccgccga
    30301 tggtgcacta ctccccgccg tacagacact gttggtttac aactcgagca accagatcgc
    30361 cgggcgtatc ttcagctata accaatcgtt tcgtgatatt ggcaacgtta ccggaccatt
    30421 gatgggagca gcgatttcag cgaactacgg tttcagagcg gtatttctcg tcaccgctgg
    30481 cgtagtgtta ttcaacgcag tctattcatg gaacagtcta cgtcgtcgtc gaatacccca
    30541 ggtatcgaac tgatttttcg cctttcatac ttgcaaaagc ggagaatcag ctatcctttt
    30601 ccctgaaacc tcatcaactc aaagggagaa tcgtgatgac catgtacgca acgcttgaag
    30661 aagccattga cgctgcacgc gaagaatttc ttgcagacaa ccccggcatc gacgccgaag
    30721 atgcgaatgt gcaacagttc aatgcccaaa aatacgtttt gcaggacggc gacatcatgt
    30781 ggcaagttga gttttttgcc gacgaagggg aagaaggtga atgtttacct atgcttagcg
    30841 gtgaagccgc gcaaagtgtt tttgatggcg actatgatga gatagagata cgccaggagt
    30901 ggcaggaaga gaatacatta catgaatggg acgaggggga atttcagctt gagccaccgc
    30961 tggataccga ggaaggacgc gcagcagctg atgagtggga tgaacgttaa tcactcatac
    31021 gggccatgat gagtgtcgat cggcagcagt aaagtatcaa aaaccagaga gaatggcaga
    31081 tcaaggatcg tgacataacg ccaggcggag tcacgcacat cccattgcac accgggataa
    31141 tattggttgc catgcccctg ccccggtata gtgcgactaa taatgctgcc acagccactc
    31201 agcaaggtca ccatgatgct caccacaatt aaacgcattt gatacctctt tcgtaaaaaa
    31261 ataccggcat aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac
    31321 gccttattcg acccacaaaa tcttgcaaaa tcaataaatt gcaggaacaa tgtaggcctg
    31381 ataagcgtag cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa
    31441 tgccagtgga ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg
    31501 ggaattccag acgcggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag
    31561 gcgacgatca cgattcagct tctctggcgt ctcgttcagc gcctgttcaa cgtggcggtc
    31621 acgggcgatt tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag
    31681 agcgttaaat gacgggttaa acactgcgtg cataaagcca tcatcaaggg agcgttgacg
    31741 attcatctca aggaaccgat cggtatcaac cagcacctgc ggcggcgaat actcttccgg
    31801 gatcaggaac agtttccagc gtttggtgcg cagaccaacg gtggcacggc tggaaatcac
    31861 cgaaacaaac ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg
    31921 cagatccagc cacgccatcc caacagccca cactaaccct aacagcagct gtgagccgtg
    31981 gcgtttgaac gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac
    32041 cacttcccag ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg
    32101 agccagcagc acggaaaaaa gcacttccag cagcaacgat aatgtaacgc gccagaagcc
    32161 gccgtattct ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa
    32221 caacagcacc atagtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca
    32281 caccgggaac aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac
    32341 ctgcaatgca gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat
    32401 cacgcccgtc aggaacaccg cacggtgaac cgggtgcata cccttaacca ggaacagacg
    32461 gaagttcatc aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt
    32521 aggcggtaat tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc
    32581 acggcgcatc aacgccgctt ctacaaagtc atgtgacagg attgaaccgg caaaggaacc
    32641 ttcgcccggc agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc
    32701 gttatgtccc cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag
    32761 tggcccatac acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga
    32821 cgctttcggc gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag
    32881 cccgcacaaa caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta
    32941 ctggctgccc cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg
    33001 acggcggcgg cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca
    33061 ggctttttgc tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc
    33121 aaagtgtttg gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa
    33181 aacacggttc acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt
    33241 taatggttca tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag
    33301 ttgcaggaag cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc
    33361 aaagaggatc aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca
    33421 caaatcctga ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat
    33481 ggtcttcata taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta
    33541 acggcggatg gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa
    33601 acgagccaga tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac
    33661 cgggttggta cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt
    33721 cagctgatcg cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca
    33781 ggcttgttcc agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt
    33841 gcggtgttcg gcatccagcg cctgatgaac ggcgcggata tcagtcttcg gcaatgccgc
    33901 tttctcgctt gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag
    33961 gtaactggta gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca
    34021 tttcagtggt tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg
    34081 taaccgggtt atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt
    34141 gcgctgtgac cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca
    34201 caaaggcgat agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg
    34261 aacgacgcgt ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc
    34321 tgaaggtgat ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg
    34381 tccagtaagc gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa
    34441 cgctgccttt accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac
    34501 gatcatcgag atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt
    34561 gcgggttttc catagagaag ctgctgaccg ctaaatgttt cgggttattc aacggacgcc
    34621 agatccattc gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac
    34681 gatagttatt tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg
    34741 caacccccag tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg
    34801 tgtcacgccc tggcataact acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca
    34861 atgcataaat ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga
    34921 agcgtggaaa ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa
    34981 ggccataaac ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga
    35041 tttcatcgtt tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa
    35101 ggtcttttac cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt
    35161 atttgattcg tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt
    35221 acataccctg atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt
    35281 tgtcatgatt aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg
    35341 gcaagttgct tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga
    35401 catcatcaat actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac
    35461 tcaaccaacg cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc
    35521 tttgtgtgct tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga
    35581 atcccggtat cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca
    35641 ctttccgtgc ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt
    35701 tatttatcaa ccgtcttaca gacgcttcca ttctggttag tggtagcgat gatgctatgc
    35761 ataaaaacct tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc
    35821 taaaggcata acccgacagg gataagacga aaaatccgag attagttaac catatatgaa
    35881 tccagtaccc gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt
    35941 agggatccct tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc
    36001 cgaaccgtca ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgcaggt
    36061 atttttcgtt atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa
    36121 atggtggaaa gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccttact
    36181 gacattgccg caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg
    36241 gctgtcattg tatgacaaat ataatacgtt ggcctgggaa ttaatatcac atctgtggtt
    36301 tttactggtg ttagtggtca tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa
    36361 taatttagaa aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat
    36421 ttttttatgc ctcggcatcg gttatgcggt aataagaaga acgattttta ttgtgtatcc
    36481 gcccattctg agtaatggca tgttcaattt tattgtcatg caaacgctgt tttatttgcc
    36541 gttctttatc ctcggcgcac tggctttcat tttccctcat cttaaagcct tgtttaccac
    36601 gccgtctcgt ggctgtaccc ttgcagcagc attggcgttt gtcgcttatt tactcaacca
    36661 gcgctatggc agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct
    36721 cggtctgtgg atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc
    36781 agcgcgggtg acttattttg ttaacgcatc gctgtttatc tatctggttc accacccgtt
    36841 aacgctgttt ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct
    36901 ctgtggcctg atatttgttg tagggattgc gataattctg tatgaaattc atttgcgcat
    36961 cccgttactg aagtttttgt tctctggtaa accggttgtt aagcgtgaga acgataaagc
    37021 accagcccgt taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg
    37081 accataaccc gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt
    37141 gcatgacgat caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca
    37201 tcatactggc tctgaataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc
    37261 acgaagccca tttcagtatt gagcaatgtt gaacgcggat cgaaattgaa agagccgata
    37321 aacaccgttt taccatcgat gctaaaggtt ttagcatgca ggctggctcc ggaattaccg
    37381 gttatgccgc gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat
    37441 tccacgccat agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca
    37501 acatcgttag cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc
    37561 cgtaagagtt gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg
    37621 cgttcactgg gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt
    37681 gccttgccct cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc
    37741 ggcaacgttc catcaaccag atgatttata aacggactgg attccatttt gcgtaagtaa
    37801 cgatgcgtca tagcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca
    37861 ccttccggga catccagcac ttgctgtaag ggtgaaaccg atttgcaata ccagtagcgg
    37921 gcgaaatcat cggcaacgtc ctctaccacg ggccctattg ccataacatc taaatccgaa
    37981 aaaagtggct cttcccctgc cccaaaatag gcatcaccaa tatttcgccc ccctaccagc
    38041 gtcaccacgc catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag
    38101 tcggtgatat aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact
    38161 tcaatgcggg gatggctgtc aagcaagcgt aaaatgtcgt caagtccggg cgtattgttg
    38221 tcgtccagca acaaacggac gcgaacgcca cgctttgctg cggctaacag ggcggaaaac
    38281 agtaaccgac ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct
    38341 gccatttcgg ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa
    38401 agaccacact gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc
    38461 aaatcattca tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc
    38521 ataacagaca aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt
    38581 tttaactgcg atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc
    38641 aggaaaagcc actgacgtat agctgtttgc cgccaccagt cgtaggctat tgagataggc
    38701 atcctgcaaa agctggtctt cgttttgttc accaccacgc cagactggcc cgacgctgtg
    38761 cactacggct ttagcgggaa gagcgcctgc aagcgtaata acggcatgcc ccgtagggca
    38821 atcgccctgc tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg
    38881 atgaatggcc ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat
    38941 cacatcaacg gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac
    39001 gcctccttac accttcttgt ttcttaagtg tacagcaggc tttttggtta aatcgcattt
    39061 ttctgctcag caacgtgcgt tgttcagaaa cttagttata ttcgatgttc attacagcca
    39121 gctttttctg tttatcaaga tactgcactt ttactgaggc tatttgcgcg tttttaacat
    39181 taccgctcat taacgctttg ctggaatagg ttttgcccgg aacagaacca ttctgtgggc
    39241 agttcatttc tacgggtgat gactgggtgc tgacgtcaca tgccggttcc acaatttggc
    39301 cataaaaatg gatcaccccc ccttgcgcgg tgcttcctgc gatggcaata aaaggaaaaa
    39361 gcaaactgcc gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa
    39421 tgattagtca tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag
    39481 accattctct ccagattcat cttatgctcg atatttcaac aaattaagac ttttctgaag
    39541 agggcggcca ttgttgtgat aaatgaagtg actgtccatc agaaacagta acaactattt
    39601 tcacccgatc ttccggggaa atatttaaac tcaacttcgt caaagcaata ggttgattag
    39661 caggcaatga gagggtcttt tcttgcttcg tctgactttg ccctgaactg ccttcgcgca
    39721 gggacaatat ttgtactctg cacagacaag attgagtaag agtgacttca ggaataatgg
    39781 tatacacatc cccttgctgg gtcgtattaa aggttatctg gctggaaagt gccgcaagga
    39841 gtaataacgc attcatattc ttctcccgaa aaaaaacagg gcttgcgccc tgtttctgta
    39901 atacaaatga tgtattagta ctgatgagcg gtcgcgttgt taccaaagcc aacctgagtc
    39961 acgttgacgg aggagttaga tgcagtctgg tcaactgcag caccgttgcc accaccgaac
    40021 tgtttaacag tcatttcaga atttttgccg ttccactgat caagagtggc gctgttaccg
    40081 aagccacgtt gggtcagatc gattgagctg tcatctgagc cctgacctac atctgcacca
    40141 ttaccaccgc catgctgggt aatagtcaag tcagagttac gggcatcagt ttgcagagca
    40201 agtgcagagt taccgccacc gtactggtaa atgttcagct ctgaatttgg gccgctatta
    40261 ttaccgccac caccgtggtt accgccgccg ccgtactgag gaacaacacc tgccagagcg
    40321 ctaccggaga atacgattgc tgcaattgct gctactttta aaagtttcat gtaaaacccc
    40381 catcggattg atttaaaagt cgaatggaaa ttaacgttgt gtcacgcgaa tagccatttg
    40441 cgactgtctc tgcactacaa ttgccgtttt ttgagtacca tactgtgtaa tatttgcttt
    40501 attaccagaa cctttctgga taatcatcgc agtattacca taagcacctt gcgaaatact
    40561 ggcatcgttg gcactgcccg cctgatcaat atatgcaagg ttataatctc ctgtctggtc
    40621 aatctttgcc cggttgctac taccttcttg cgcaacaacc gccaaaagtt ttgagcctcc
    40681 ctgccgtaac tgagcactat tattcgtccc agcttgacca attatggctg cctgattaaa
    40741 tgaagactta ctcaattcat ttaccgcgaa gttatattct gaattagcta aatcataacc
    40801 tgctgcggct gcaatcccag gcgcacccag tattgttaac atcataaata acaatttgtt
    40861 tttcatgttg tcaccctaga cctggtcgta catttaagaa attaaatcat ttcaacttgg
    40921 ttgttaacgc aacctgtatt ttgttaacgc tgcgttacga tggaaagtat gtctgcggaa
    40981 atatttttaa taactcaccc gcgcgttgta tttttctttt tttgttcata ccaaagtatt
    41041 aaaacccacg taaatacgct gttatctacg caaaaaatat tttgttttct tttaaatctc
    41101 cgttttccgc tatcaaaaat caccagacag tcatttttct tgcccgtcgc tgattgctgc
    41161 gatatgtctt gcgcacaagc cgtgacaaac ctcgtctttt tgcagtaaaa aattgtccac
    41221 ggaggtgcgg ag
//
LOCUS       DAZGXZ010000025        41032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000025 DAZGXZ010000000
VERSION     DAZGXZ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.025"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(321..1229)
                     /locus_tag="ACHKJF_001523"
     CDS             complement(321..1229)
                     /locus_tag="ACHKJF_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFP0948905.1"
                     /translation="MLGALKLVDEPVSAMVSRIVTDLRVPRMLLSVLTGAGLAMVGAL
                     LQTTTRNDLADPFLFGLSSGASAGAVLVITRFGDRLGVLTLPVSAFVGGVCSAIAVML
                     LFHFKKQRGAEHLILCGLAISFLFGALTSYFIFSGDQRAASSVLFWSLGGLGLATWNN
                     LPFAVFSLVLLFAFVLLRWRSLDGVLAGEQTALSLGINVSRLRIEIFLCCALATSLMV
                     ALTGVIGFVGLMVPHLCRHFAGVKHLLLLPLCGVWGAILLCGGDIVSRTILAPQELPI
                     GIITAGIGGLFIIMLLARNSSRNTAC"
     gene            complement(1340..2296)
                     /locus_tag="ACHKJF_001524"
     CDS             complement(1340..2296)
                     /locus_tag="ACHKJF_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0948906.1"
                     /translation="MKKLFISLLAVLSASVASAADFPVTIESCGTPVTFAGPPKRAVI
                     NDLNMSEMAFALHLQDRIVGLTGISGWYKMTPEFKKAMGSIPELAPKYPTLETLLAAE
                     PDFFFAGWNYGMKVGGEVTPDTLSKYGIKTFVLSESCVFTTAHKNKATMDLLYNDVLT
                     LGKIFGKRNDAQSLVSGWKKRLNELPKPAAGTRPLKVFVYDSGEDKPFTSGKYAMPTA
                     IIEAAGGKNVMEAVDTSWGTTSWESVAATEPDFIILLDYQTGSGADALRHFLENHPLM
                     KLTPAVQHHRYLKLQYAELTPGPANVDAVEKLARAMYPSTAK"
     gene            complement(2318..2635)
                     /locus_tag="ACHKJF_001525"
                     /pseudo
     CDS             complement(2318..2635)
                     /locus_tag="ACHKJF_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851089.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2809..4071
                     /locus_tag="ACHKJF_001526"
     CDS             2809..4071
                     /locus_tag="ACHKJF_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0948907.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAERGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLLKGIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(4265..5569)
                     /gene="ybtS"
                     /locus_tag="ACHKJF_001527"
     CDS             complement(4265..5569)
                     /gene="ybtS"
                     /locus_tag="ACHKJF_001527"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFP0948908.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCLARLHINADGTQATFIDDAGEQQWAVDSITDCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGGRRLCEWVKEASTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCLPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDMRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(5597..6877)
                     /gene="ybtX"
                     /locus_tag="ACHKJF_001528"
     CDS             complement(5597..6877)
                     /gene="ybtX"
                     /locus_tag="ACHKJF_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFP0948909.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LMIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRAGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAMLIMALSFPLWRITEPTRTAPI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTFMMAPGHLLLPILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGVFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(6870..8672)
                     /gene="ybtQ"
                     /locus_tag="ACHKJF_001529"
     CDS             complement(6870..8672)
                     /gene="ybtQ"
                     /locus_tag="ACHKJF_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFP0948910.1"
                     /translation="MKDNNPADNLAWRVNWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIERFMAIAPLPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVIEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGVSASGEWVHE"
     gene            complement(8659..10371)
                     /gene="ybtP"
                     /locus_tag="ACHKJF_001530"
     CDS             complement(8659..10371)
                     /gene="ybtP"
                     /locus_tag="ACHKJF_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFP0948911.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSED
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            10628..11587
                     /gene="ybtA"
                     /locus_tag="ACHKJF_001531"
     CDS             10628..11587
                     /gene="ybtA"
                     /locus_tag="ACHKJF_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFP0948912.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRVM"
     gene            11778..17885
                     /gene="irp2"
                     /locus_tag="ACHKJF_001532"
     CDS             11778..17885
                     /gene="irp2"
                     /locus_tag="ACHKJF_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFP0948913.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQKLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNHHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCITDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPIRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLAAHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     AFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLT
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYVDG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQQQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKQAGQCAERSPRVCPEQSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            17973..27464
                     /gene="irp1"
                     /locus_tag="ACHKJF_001533"
     CDS             17973..27464
                     /gene="irp1"
                     /locus_tag="ACHKJF_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFP0948914.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREVLLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYQHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPTAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPERFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDQPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENPPWETLHQGLSAVSLSQRWLAARGDTLWLPSLAPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHLIGYGGVACHVLFEWDKRHDEFNLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPVVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIAGHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQSQWSQALAQYLETCI
                     ARHDALFSGRCSPLELLFNEQHRVTDALYRDNPASSCLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDTPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGEQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPARWTPAETEWQGW
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSMQPENTL"
     gene            27461..28561
                     /gene="ybtU"
                     /locus_tag="ACHKJF_001534"
     CDS             27461..28561
                     /gene="ybtU"
                     /locus_tag="ACHKJF_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFP0948915.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITKMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLYRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            28558..29361
                     /gene="ybtT"
                     /locus_tag="ACHKJF_001535"
     CDS             28558..29361
                     /gene="ybtT"
                     /locus_tag="ACHKJF_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFP0948916.1"
                     /translation="MTQSAICIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDREASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTALQKQPSTSER"
     gene            29365..30942
                     /gene="ybtE"
                     /locus_tag="ACHKJF_001536"
     CDS             29365..30942
                     /gene="ybtE"
                     /locus_tag="ACHKJF_001536"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFP0948917.1"
                     /translation="MNSSFKSLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRVGVIPVLAMPSQRALD
                     IDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETVSDDFTPFFSLHGER
                     QAWPQPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            31073..33094
                     /gene="fyuA"
                     /locus_tag="ACHKJF_001537"
     CDS             31073..33094
                     /gene="fyuA"
                     /locus_tag="ACHKJF_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFP0948918.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATMDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            33744..34493
                     /locus_tag="ACHKJF_001538"
     CDS             33744..34493
                     /locus_tag="ACHKJF_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFP0948919.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLLVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVQAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            34420..36542
                     /locus_tag="ACHKJF_001539"
                     /pseudo
     CDS             34420..36542
                     /locus_tag="ACHKJF_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(36712..37764)
                     /locus_tag="ACHKJF_001540"
     CDS             complement(36712..37764)
                     /locus_tag="ACHKJF_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP0948920.1"
                     /translation="MFLVSLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRTPVPMTWAFMMREAGFPVVRSIYELPLSDDVLDEVREEMRSLGSYIALNLDGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            38079..39395
                     /gene="shiA"
                     /locus_tag="ACHKJF_001541"
     CDS             38079..39395
                     /gene="shiA"
                     /locus_tag="ACHKJF_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP0948921.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDVQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGMLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            39497..40951
                     /gene="amn"
                     /locus_tag="ACHKJF_001542"
     CDS             39497..40951
                     /gene="amn"
                     /locus_tag="ACHKJF_001542"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP0948922.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         9422 a        11850 c        10847 g         8913 t
ORIGIN      
        1 tgaccacctc attcatactt acattgagaa aaaagcaata gattatggtt gtgctgtaca
       61 aaaaaaagat attgaagtta aagtaatttg ccctgattgt cgaggaaagg gttaaatcac
      121 caatacgaat aatcattgaa atcgagtcag gcgataaata aattttaaaa tatctaaaag
      181 ccatcacact attacctgac tctataataa atataaccgt caacaaggat tgtcggttgc
      241 tttcataatt ggcgtcaaca acctcaacct tcagaatgaa taaaagacat cagagcctta
      301 tttttctgtc ttatatgtgc ttaacaggcc gtatttctgg acgaatttcg tgcgagtaac
      361 atgataataa acaaccctcc aatgcccgcc gtaataatgc caattggtag ttcctgcggt
      421 gccagtatcg tccggctgac tatatcgccg ccacacagta atattgcacc ccagacgcca
      481 cacaaaggta acaacaacag atgcttaact cctgcaaaat gccgacacag atgaggcacc
      541 attaagccaa caaatccaat cacgccggtt aatgccacca tcaacgatgt tgccagtgcg
      601 caacaaagaa aaatctcgat gcgcagacga ctcacgttaa tccccagcga caacgctgtc
      661 tgttctcctg ccagcacacc atccagagag cgccaccgta acaggacaaa agcaaacagc
      721 aacaccaggc tgaacactgc gaaaggcaaa ttattccagg tggctaatcc cagaccaccc
      781 agtgaccaga acagcacaga gctggcagct cgctgatcgc ccgaaaaaat gaaataactg
      841 gtcagcgccc cgaacaaaaa agagatggca agaccgcaga gaattagatg ctcagctccg
      901 cgttgctttt taaaatgaaa caacagcatt actgctatgg cggaacacac accaccaaca
      961 aacgccgata cggggagcgt taatacacca agtcgatcac caaaacgggt gatcaccagc
     1021 accgcccccg ctgacgcgcc ggaggatagc ccgaacaaaa aaggatcggc cagatcgtta
     1081 cgggtggtag tttgtaacaa cgcccctacc attgccagtc ccgcccctgt cagcacagag
     1141 agcaacatcc tcggtacgcg taaatccgtc acaattctgc tgaccatggc agataccggc
     1201 tcatccacca gcttcagtgc tcccagcacc tacattagcg acagtgaaac tgtccctctg
     1261 gtaatactga ataacatcaa cccaatcagc actaccatgg ctccccacat cgcccagtga
     1321 taacggtacg acatgagcat cacttcgccg ttgacgggta catcgcccgc gcgagttttt
     1381 ccaccgcatc aacgttggca ggccccggtg tcagttcagc atattgtaat ttcaggtatc
     1441 gatgatgctg aacagccgga gtcagtttca ttaacggatg attctcaaga aaatgacgca
     1501 gtgcatccgc accgctgccc gtctggtaat ccaacaaaat aatgaagtcc ggctcagtag
     1561 cggccacgct ttcccaggaa gtcgttcccc aactggtatc caccgcctcc attacatttt
     1621 taccaccagc cgcctcaatt atggccgtag gcatcgcata tttaccactg gtaaacggtt
     1681 tatcctcgcc tgagtcgtag acgaacacct ttaaaggacg ggtacctgcc gctggcttcg
     1741 gcagttcatt caacctcttt ttccagccgc tgaccaggga ctgtgcgtca ttgcgcttgc
     1801 caaaaatctt acccagcgtg aggacatcgt tatacagcag atccatggtg gctttatttt
     1861 tgtgcgcagt ggtgaacaca cagctttcac tcagaacaaa agtcttgatg ccatatttgc
     1921 ttagcgtatc cggcgtcact tcgccaccca ccttcatccc atagttccag ccagcgaaaa
     1981 agaaatcagg ttcggctgcc agcaatgttt ccagagtggg atatttgggt gccagttctg
     2041 ggatcgagcc catcgccttt ttaaattccg gcgtcatttt gtaccagcca ctaataccag
     2101 tcagcccaac aatacgatcc tgtaaatgca gtgcaaacgc catttctgac atattaaggt
     2161 cgttgataac agcccttttc ggcggaccgg cgaatgtgac aggagtccca caactttcaa
     2221 tagtaacagg aaaatcagca gcactggcta ctgaggcaga cagtaccgcc agcagggata
     2281 taaacaattt tttcatcact gactctcaga aagtaattta tgcacaatgc ggaacttcaa
     2341 aaatacgcaa cgcctttcct gtatggggat gcggtacggt aaaactggtc aagccaaaga
     2401 tgggataaag gtattctgat accaataccc tctccggtgt gtcacagatc actgattgcc
     2461 cctgcgacaa gaggagaaca cggtcagcaa atgattctgt cagtgtcaga tcgtgcaata
     2521 ccgccacgac ggtaattccc ttggttttta caagggaaag tagttcctcg cgcccgggag
     2581 gatcgagatg attggtgggt tcatcaagca ataccaggtc gggcgtctgt gccagtcaga
     2641 ggagccaatt ttctgttttc atgcttcctt gcgaatcctt atctgatgtt gattcaacag
     2701 gttagtgtga aaactcttcc cggtgcattt tgattttacc ctacgcatcg ggaaaaattg
     2761 gtggtcaaat ctggggtcag gttagttcga taatggagtg acccccatat gtcccttacc
     2821 gacgcaaaaa tccgcaccct caagccttct gataaaccct ttaaagtctc cgattcccac
     2881 ggtctgtatc tgctggtcaa gccgggtggc tcccgccact ggtatctcaa ataccgtatt
     2941 agcggtaaag aatcccgcat tgcgctgggt gcctatccag ccatctccct gtctgatgcg
     3001 cgacagcaac gtgaaggtat ccgtaaaatg ctggcgctga atatcaaccc ggtacagcag
     3061 cgggctgctg aacgtggctc acgaacaccg gagaaagttt ttaaaaacgt ggcgctggcg
     3121 tggcataaaa gtaacaggaa atggtcgcag aacaccgccg accgtctgct tgccagcctg
     3181 aacaatcaca tctttccggt catcgggaac ctacctgtat cagaacttaa accccgtcat
     3241 ttcattgacc tgctgaaagg gatcgaggaa aaaggtctgc tggaggttgc gtcccgcaca
     3301 cggcagcacc tgagtaacat aatgcgccat gcggtccatc aggagttaat cgatacgaac
     3361 cctgcagcaa accttggcgg cgtgaccaca cctcctgtca gacggcacta tcctgccctg
     3421 ccgctggagc ggctgcctga actgcttgaa cgtattgggg catatcatca gggccgtgaa
     3481 ctgacccggc atgccgttct gctgatgctg catgtgttca ttcgctccag tgaactgcgt
     3541 ttcgcccgct ggtcagagat tgatttcaca aaccgagtct ggacgatacc cgcgacgcga
     3601 gaacccatta ttggcgtgcg ttattccggc cgcggggcaa aaatgcgaat gccgcatatc
     3661 gtccccctct cagaacagtc catcgccatt ctgaaacaga ttaaggatat caccggtaat
     3721 aatgaactga tcttccccgg cgaccataac ccgtataagc caatgtgtga aaacacggtc
     3781 aataaggcac tgcgggtgat gggttacgac acgaaaaagg atatctgcgg tcacggcttc
     3841 cgggcaatgg catgcagtgc gctgatggaa tcgggtttat gggcaaagga cgcagtagaa
     3901 cgccagatga gtcatcagga gcgcaatacc gtgcgcatgg cttatattca taaggcagag
     3961 cacctagaag cccgcaaagc gatgatgcag tggtggtcgg attatctgga agcatgccga
     4021 gaatcttatg caccgcctta tacaattggt aaaaataagt ttatcccata gtatactaac
     4081 gaatcccaca ctctggcaaa ttaccaccac ctctacagga ggtggttcta tatagacttt
     4141 tatcaacttc atgacgaaaa acacctattt tatccaaaaa gaagacaata gagtcacaga
     4201 ggaatcaaag agcgactcaa ttgtctttgg acagtctggt tgtgaggtta gaaaacagtt
     4261 actcctacac cattaaatag ggcgcaatgc tcgctaattt ctcccgggtt tccgtcagtt
     4321 cgcgttccgg tgtagattgc gcgatgattc ccgcccccgc ttgtatccag cagcgctggc
     4381 tgtcctgaaa tacggaacgc agaactagcg ccgcatcgaa gcgcatatcg tccagcagca
     4441 gaattgcgcc ggaatagagc tctcgcggcg tcttctcaat ttgcataatc gcgttcaggg
     4501 cagcgttttt agggatacct gaggcggtaa tcgacggaaa cagcacggta aacgcatccc
     4561 aggcatcctt gttttccgca agctgaccgc tcacgccgga ccccagatgc tgaacgctgc
     4621 cgcgctggcg aaccgacatt aaatcttcaa ccaccacgct gcccggcagg caaacggcct
     4681 ccagttcagc aatcgcttct ttgacagaaa ggatatgctc aagcacctct ttactgtcgt
     4741 gcagcagttc tgcctctttc gccttattat gctccgggtt tcccatgcga tcgcgggtgc
     4801 ccgcaagcgg ttcagtgacc actttattgc ccgtcactga catcaccagt tccgggctaa
     4861 agcccagcgc ttcgcgtcct tcctgacgga acataaacga gcgcacaggt gtatttgcct
     4921 gccgcccgta taacagcgtg gcgggcatat caatccgcga tggcagggga atagcgcgcg
     4981 agacaatcac tttgacatac tcgccacggc ggatctccgc aacggcgcgt gcaacctgtt
     5041 gtttatacgc ctcgccattg agggcggtat ccaccgccag tggtgcgttc tgcgtcgttg
     5101 tactggcctc ttttacccac tcacacaaac gtcggccccc gtcggcggag tccgcataaa
     5161 cggtgacatt tcccttttca aaaataagtt cttcacgggg aacggttaac gtcagcagcg
     5221 gccactcccc ggcgttaaag gcaatccccc gcgcatgcgc cgcaaagttg aacccaacct
     5281 gtccataaac ccgacgtcct ttgacctgag gatgtgccat aaaacgacgc gcgcagtcgg
     5341 taatggaatc caccgcccat tgttgctccc cggcatcatc aataaatgtc gcctgcgttc
     5401 cgtcggcatt aatgtgcaac cgtgccaggc accctttgcc taaataccaa cagggttgac
     5461 gctcatagac ataacactct tcttctgcga actggcgtaa aacgccagaa atcgtcggca
     5521 gccattgttc ctctggtaac gccaggtgta aaaattcact gattttcatc ccttacctct
     5581 ctgtgttatt cccggatcaa tcaattgcgc ttgtcatcag ttctcttgcc gaatgcagcc
     5641 ggatataagc aacccacgcc gccagcaacg tgaatacccc ggcaaacagg aagcaggccg
     5701 catagccaaa atgttgagca acaacgccgc cgataacacc agccaggatg gcgattgccg
     5761 cgtcagtaca ttgaaagagg gtgaagtcga caccggcctg caaaggcgat gaaagcgaca
     5821 tcagcgtggc gtaaagacag accagcgcgc aggccagcga aatggactgg acaatgacca
     5881 gacactggag aatcggcagc aacagatgac ccggcgccat catgaacgtc atcaccaccg
     5941 ccagcagcgc gatcccctgg acgccataag ccgtcaacag cgctctgccg ggtgaggtgt
     6001 atttcatcag taatccgccg gccagcgttc ctgctatgcc cgctgcaata ttgccgccgc
     6061 tgaacagcgc gcccaacgca gacatgctca acccatgatc caacaacagc ggcgccagaa
     6121 gaggcagcac aaaccgcatg cctgaattca gcattaatac cagcagtaag cccaggcgcg
     6181 cctgcttcct ccttagcgca taacctaacg ccggaacatg cgggataggc gctgttcgcg
     6241 tcggctccgt aatgcgccac agcgggaatg acagcgccat aatcagcatc gccatcatca
     6301 gcatggcgac aggccagccg gatgctgccg acaacatcag gaacaccccg ccgccgcaca
     6361 tcattcccag atagctgccg ccgacctgca cgctgtttcc ccagccgtaa cccgcgcgag
     6421 tcagttgatc gacgcaaaag ccgtcgctgg cgatatcaat ggtggaagcg accgtgcctg
     6481 ccaccataaa cagaacaaat aacgcgacga tttgcgtatc gggaaatcct ccttctcgcc
     6541 caaaccagcc aatcgctgcg gcaatcatca ggatcgccgc tagcgcgacc tgaccacgca
     6601 ggattaacat ccgcgaacgg cgttcctggc taccgggcgg aagtcgccag cgttcgatcc
     6661 acggcgccca aataaacttc agcgcccagg gcagcatgaa cagggttgtc gcaccggcaa
     6721 gcgccagcga tccgccagca gcgcgcacca gcgcgggtaa ggactgcata gacagcgccg
     6781 ataccagact ctgcgtcaca tagacgccgg caatagcaaa aatcacccgc ccggtcgtca
     6841 acgtcagcgg tttcacattc gactgaacat cactcatgca cccactctcc agacgcggaa
     6901 accccgtcgt cgcgccacac gcgcgcggcc atttgcgcct gccacagcgc ctgataacgt
     6961 ccatgatgtg agagcaattg cgcatgagta ccctgctcaa ccacctgtcc ctcttctatc
     7021 accagaatgt ttccggcccc ggcgatggtg gataaacggt gagcgatgat aatcaccgtc
     7081 cggttgtgaa ccaggttatc gatcgctttt tgcaccgcca gctcgctttc aatatccagc
     7141 gcggcagtcg gttcatcgag aatgacgacc ggcgcgtttt tcaataacgc tctggcaatg
     7201 gaaatccgct ggcgctcgcc gcccgatagc tggccgccca tctctcccat tggcgtcagc
     7261 cagccttgcg gaagacggga aataaactca aggcactgcg ccgcgcgggc ggcctcttct
     7321 acctcctgcc gcgtcgcctg tgggcgcgcg atacggatat tcgccagcag cgtgtcatca
     7381 aacagccaga catcctggaa aacaacagaa atcaggctat tgagctgttc cggcgtcagg
     7441 cggcgaatat cgacgccgcc aatagaaatc tgcccttgct gcggatcggc atagcgcatt
     7501 aacagtttgg tgaccgtagt tttgcctgcg ccggaggcac ccaccagcgc gctcatactg
     7561 gctgccggga acgtcaaaga aacatgatta agcgcgtggc cgtcgccttc ttcgtagcga
     7621 tagctgacgt tgtcaaagcg gatatcgtaa cgttccggca tctcgctttg ctctgcgacg
     7681 ggaagcggtg ctatcgccat aaaccgctca atccgttgca gggcgctggc gatcagttcc
     7741 acaaccgagg tgtagctgat aaacatcgcc atcggttctg cgaagcgcat aatcatcgcg
     7801 acggcggcaa tcaaaaaggc gaggttcaga gtgcccgtca ctacccagac gatcccggat
     7861 aacaccacca cctgtaggcc caactccacg acgctggcga tcagcatcgt cgcgccagcg
     7921 ccctgacggt gagtgcgggt ctgtaagttt tccagcgcat tgaaatgcgc cagcagcgcc
     7981 cggcttttat cggcatcgct gccgcaggtg cgcaatacca tcatcccctg agcaaattca
     8041 acgatatcgc cgctcagacg ctggtgcgct tcccccagcg tctgcatttg tcgccgcatc
     8101 gccgggcggc gccagtaata aaacggcacc agcagaggga agatcagcaa catcaccagc
     8161 cccagccgcc agtctatcca caatgtcgcc agcgacgccg tcagcggcgt gacaatggtg
     8221 agcagcaaaa tattggctat cgcaataaca taattgaggt tttcatccac gctgcccagc
     8281 agcaaggcgt tcatttcacc cgccctgccg cgctggagct tctccagcgg cacgcggcgt
     8341 aactgctcgc caagtcgcag gcgcaactca tgggtggcct gcgccagatg accacggtat
     8401 tcaaagccca ggccatacca gcgtagcacc agcgtcacaa ttgcggcgac gctgaaggcc
     8461 atagcccagt taagaagttg cggcgcgtct ccccgtaaca gcgcatcaat gatcggataa
     8521 agacaggcaa aggcgatccc ctgcatgaat gccgccagca acagcgccag catactgcgt
     8581 cgcagcatcc tggcctgact gccaacgctg gagataagct ggcgccagtt gacgcgccag
     8641 gccaggttat ccgcaggatt attgtctttc attttccacc acctcttcct gtgttccacc
     8701 cggcacccaa tgctgcgcct gctgatagtg ttgccagagc cgttgataca ggccgccctg
     8761 cgccaacagt tgcgtatggt tccccatttc cctgagctgt ccgtcggaaa acagcaatat
     8821 cacatcggcc tgagtcacca ttgagagacg atgcgcgacc atgatgaccg tccggccacg
     8881 catggccgcc gcgagcgcct tgataagcgc cgcttcgttt tccgggtcag caaacgccgt
     8941 cgcctcatcg agcaccagga tggggcgatc ctgcaaaagc gcccgggcga tagtaatgcg
     9001 ctgccgctgg ccgccggaga gaaatacccc acgttccccg actcgtgtgt tgtaaccttc
     9061 tggcagagcg ctaataaaat catgggcctg cgccactctg gccgccgcta ttaccgcctc
     9121 cagcggcgta tccggcgcgc ccagacgaat gttattggct atcgtgtcgg caaaaaggaa
     9181 gttgtcctga aacacaaacg agagttgctt catcagggtg tccgtctgca tatcacgcag
     9241 atccacgcct ccaatacgga tatggccttt gtccgggtcg gcgtaacgca gcagcaagcg
     9301 cgccacggtg ctttttccgg cgccgcttgg cccgaccagc gccacaattt gcccggcagg
     9361 cacatgaaag ctcacctcct gcaacgcggc gccagtacgc gcttgcggat aatgaaagct
     9421 cacctgctca aaggtaatgc tcgcctcttg cggctgctgg tcagactgcg gcagcgataa
     9481 ctccggcatc gcgagaacct gataaatacg ctgaatgctt aaacgcgttt gcgcgaccag
     9541 gttattgagc atcatcattg gcattacggc ttcggccatt ccgctgccca gcagtaaaac
     9601 ggccacccac gcgataaaat cgaaactgcc atagtgcagc aggccgtatc ccgaccagat
     9661 caggacaaac agggtcggga gaggattcag aatcgagaag gaaaaacgcg ctgaaaaacc
     9721 ggctttgcga taccaggttt tgagcacatc gacccactct tcaagggcgc gttgatagcg
     9781 caaaaaactg gtgctgccgc tatcaaacgt tctcaccacg ggcatggcct gcacgaactc
     9841 aatcaccgcc gcgctaacct gctcgcgcgc cttatgataa cgctgagcca tatcttccga
     9901 acgctggcgg gcgagcacga gaacaaccga tccaaacgcc agtaccccca gcgtcgcgat
     9961 tgccagccgc cagtccagcc agaacaagat aacgattgtc gccagcggca tgatgatcgc
    10021 gcgggcatag agcggcgtgc tgtcggccac aaaaatatgt aacgacttca cgtcatccag
    10081 catcactttc gccactgacc cgctgcccat ttgctgtaac acgccaggcg gaagctgcaa
    10141 tgctttacgg gctaacctgc tgcgcagaat gttctccaga tgaaaagccg cgtaatgaga
    10201 ggtgttgaac gcctgtaagc gcagaacaaa agttaagacc acgcagccga tcacgccgcc
    10261 cagcggccag gccggaatag cgtcaggcgt cgcgcgaatg tcacgcaggc tccacgccag
    10321 aaaaaggagc gcccccaggc tggttaatgc cgccagcccc gccagcgcca tagctaaaat
    10381 aaccttcctg cgaaccggtg taatcacctg ccagagagga aatcgggaca gcgattcagt
    10441 attggatgat tgagatgaca tgggagtaac tgaatttcct gatgaatttt tacggacgcc
    10501 aaaaacgaca attcagggtg gcgttctgag aattaatgtg aataataacc attatcaata
    10561 actcatctac cccattcggt tctatttata cccgcattgg tctaagccac agggagataa
    10621 ccaggtcatg acggagtcac cgcaaacgca atctgaaatc tctattcacc agttggtggt
    10681 cgggaaacct gcaaacgatg gcaatattcc tgctcagtgt gaactgctgc gctgttcgtt
    10741 gcaggaaggt atggatattt tgctctggag gggccatttt gcgcggcctg agacgttgca
    10801 gctacacgac gatctgggca ggattaactt ctcgtgcatc ctggaaggca cttcgcgctt
    10861 tgcgattcag ggactccggc gccacaccga ctgggagctt gcgcgcaatc gccattacat
    10921 tacccatacg ccagactgtc gcggcagcgc ctcctattgt ggccgattcg agagcattac
    10981 cctctccttc agccctgaaa ccctcgcgct gtgggtgccg gatattagcg cggtcattaa
    11041 gaataagatc gactcccact gctgttgcca gcagcagcgt tgtaatgctg aaacccactt
    11101 aacggctcag gccttgcgtc acgcgctgat gcgcatgcac ggcggcttca gtcatgaaca
    11161 aaagccgtcg acgctatggt tgcaggggca aagtctggtc atgctcagtc tggtgctgga
    11221 tgaacatcgc gaagacgcca gttgcctctc ctgccacttc aacccgatgg aacgccagaa
    11281 actgctgcgg gcgaaggatc tgctgctggc tgatctgacg caagcgccgg gcgtcgcgga
    11341 gctggccaga gagtccggtc tgagcgttct caaaattaag cgaggcttcc gcgttctgtt
    11401 caacaacagc gtctatggcc tgtttcaggc tgaacgaatg caggaagcca gaagacgcct
    11461 ggccaacggc aatacctccg tcatgacggt ggcggccgat ctgggctatg ccaacgccag
    11521 ccatttctct gccgccttcc agaaacagtt tggcgtaacc ccttcgacct ttaaacgcgt
    11581 gatgtaaccg gggtcgcgcc cccctaaact cctccctgac agaggcccgg ataccccatc
    11641 cgggcctctg ttctttccgc ctgttaccaa cccgaaaggg gcataaataa acccgtttcg
    11701 ggtagcatcc aattgttaat aattattatt ctcatatgag caatgctttt cggtaagacg
    11761 tgccatcagg aggaagaatg atttctggcg caccatctca ggattcgctg ttaccggaca
    11821 accgccacgc ggctgattac caacaattac gcgagcggct catacaggaa ctgaatttaa
    11881 cgccgcagca gttacatgaa gagagcaacc tgatccaggc cggcctggat tccataagat
    11941 tgatgagatg gttacactgg tttcgtaaaa atggctaccg ccttaccctt cgcgagctgt
    12001 atgccgcccc cacgctggcg gcatggaacc agttaatgct cagccggtcg cctgagaacg
    12061 cggaagaaga aacgccgccc gacgaatcat cctggccgaa catgaccgaa agtaccccct
    12121 tcccattgac gccagtacag cacgcctacc tgacgggccg catgccgggg cagaagcttg
    12181 gcggcgtggg ttgccacctg tatcaggagt ttgaaggcca ttgtctgacg gcgtcgcagc
    12241 tggagcaggc catcacgacc ttgctgcaac gccacccaat gctgcatatc gcctttcgcc
    12301 ccgacgggca gcaggtctgg ctaccgcaac cttactggaa cggcgtcacc gttcatgatt
    12361 tacgccataa cgacgctgaa agccgccagg cctatctgga cgcactgcgc cagcgcctga
    12421 gccaccgtct tttacgcgtg gaaatcggcg aaacgtttga ttttcagctg acgctcttgc
    12481 cggacaatca ccaccgcctc catgtcaata ttgacctgct gattatggat gcctccagct
    12541 ttacgctttt cttcgatgag cttaacgccc tgctggccgg agaatcgctg ccggctatcg
    12601 acacccgcta tgatttccgc tcgtatttgc tgcaccagca gaagatcaat caaccactga
    12661 gagacgacgc gcgcgcttac tggctggcga aagcatcgac gcttcccccc gcgcccgtct
    12721 tgccgctggc ctgcgaaccc gccacgctac gtgaagtccg taatacccga cgccgcatga
    12781 ttgtcccggc aacacgctgg cacgccttta gcaaccgggc cggcgagtat ggcgtgacgc
    12841 cgacaatggc gctggcgacc tgtttttctg ccgtgctggc tcgctggggc ggcctgacgc
    12901 gtctgctgct taacatcacc ttattcgacc gccagccgct gcacccggcg gttggcgcga
    12961 tgcttgccga cttcaccaat attcttctgc tggataccgc ctgcgatggc gataccgtca
    13021 gcaacctggc gcgtaaaaac cagctcacgt ttacggagga ctgggagcat cgccactggt
    13081 ccggcgtcga attactccgt gaactcaaac gccagcagcg ctacccccac ggcgccccgg
    13141 tggtatttac cagcaatctg gggcgttctc tctacagcag ccgcgcagaa tcgccgttgg
    13201 gcgagccgga atggggcatc tcgcaaacgc cgcaggtctg gatagatcat ctggcgttcg
    13261 agcatcacgg cgaggtctgg ctacaatggg acagcaacga cgcgctgttc cctccggcgt
    13321 tagtcgaaac attgttcgac gcctactgcc agttgattaa ccaactctgc gatgacgaaa
    13381 gcgcctggca aaagccgttc gcagatatga tgcccgccag ccagcgcgcg atacgcgaac
    13441 gggtcaacgc caccggcgcc cccattcccg aaggcttgct gcatgaaggc attttccgta
    13501 tcgctctgca acagccgcag gcgctggcgg taacggacat gcgttatcag tggaattatc
    13561 atgagctgac agactatgcc cgccgttgcg cgggcaggtt aatcgagtgc ggggttcagc
    13621 ccggcgataa tgtggctatc acgatgtcga aaggcgcagg acaacttgtt gcggttctgg
    13681 ccgtcctgct ggccggggcg gtttacgttc cggtttcgct ggatcagcct gccgcacggc
    13741 gcgagaaaat ctacgctgac gccagcgtcc ggctggtgct catttgtcag cacgacgcca
    13801 gcgccgggtc agacgatatt cccgtccttg cctggcagca ggccattgag gcggagccga
    13861 tcgccaaccc ggtagtacgc gcccccacgc aaccggccta cattatctac acctccggct
    13921 ctaccggtac gccgaaaggg gtagtcattt ctcaccgggg agcgcttaac acctgttgcg
    13981 atatcaatac ccgctatcag gttggcccgc atgacagggt gctggccctc tccgccctac
    14041 attttgattt atcggtttac gacatttttg gcgtactgcg cgcgggcggc gcgctggtga
    14101 tggtgatgga aaatcaacgg cgcgatcctc acgcatggtg tgagctgatc cagcgccatc
    14161 aggtcacgct ctggaacagc gtcccggcgc tgttcgatat gctgctgacc tggtgtgaag
    14221 gtttcgccga cgccacgccg gaaaacctgc gcgcagtgat gctttccggc gactggatcg
    14281 ggcttgacct ccccgcccgt tatcgggcct tccggccaca aggacaattt atcgcgatgg
    14341 gcggcgccac cgaggcgtct atctggtcta acgcctgcga aattcacgac gtccccgccc
    14401 actggcgttc catcccttac ggttttccgc taaccaacca acgctaccgg gtggtggatg
    14461 aacagggccg ggactgccct gactgggtgc cgggtgaatt atggattggc ggcattgggg
    14521 tcgcggaagg ctatttcaac gatcccctgc gtagcgagca gcaatttttg acgctcccgg
    14581 acgagcgctg gtatcgcacc ggcgatctcg gctgctactg gccagatggc acaatcgagt
    14641 tcctcggtcg tcgcgacaag caggtcaaag tcggaggata tcgcatcgag ctgggcgaaa
    14701 tcgaaagcgc gctcagccag ctggcggggg tgaaacaagc aaccgttctg gcgatcggcg
    14761 aaaaagaaaa aacgctggcg gcatacgttg ttcctcaagg cgaggctttt tgcattaccg
    14821 atcatcggaa cccggcactg ccgcaggcgt ggcacacgct tgcgggaacg ttgccctgtt
    14881 gcgccatctc gccagagatc tccgcagaac aggtagccga tttccttcag catcgcctgc
    14941 taaaactgaa gccgggtcac accgctggcg ccgatcctct ccccctgatg aactcactcg
    15001 ctatccagcc gcgctggcag gccgtggtgg aacgctggtt agcatttctg gtgacacaac
    15061 ggcgactgaa gcccgctgct gaaggttatc aggtctgcgc tggtgaagaa cgcgaggatg
    15121 agcacccgca cttcagcgga catgatttaa cgttatcgca aattcttcgc ggtgcccgta
    15181 acgaactgtc gttactgaac gacgcgcagt ggtcgccgga aagcctggcc tttaaccatc
    15241 cggccagcgc cccgtatatt caggaactgg cgacaatttg ccaacagctt gcacagcgct
    15301 tacagcgccc gatacgcctg cttgaggtgg gaacccgcac tggccgcgcc gcagaatcgc
    15361 tgttagcaca gctcaacgcc ggacagattg agtatgtcgg gcttgagcag agccaggaga
    15421 tgctgctgag cgcccggcag aggctcgccc cctggcctgg cgcccgtctg tccctctgga
    15481 atgcagacac gctggcggcg cacgctcact cggcggacat tatctggctt aataacgccc
    15541 tgcatcgtct gctgccggaa gatcccgggc tccttgcgac attacaacag cttgccgttc
    15601 ccggcgcgct gctctacgtg atggagtttc gccagttaac gccgtccgcc ctgctcagca
    15661 cgctcctgtt aaccaatggg cagccggagg ccttgctgca taacagcgcc gactgggcgg
    15721 cattatttag cgcggccgcc ttcaactgtc agcatggcga tgaggtcgcg gggttacaac
    15781 gcttcctcgt acaatgtcct gacaggcagg tgcgccgcga tccccgtcaa cttcaggccg
    15841 ccctcgctgg acgtctgccg gggtggatgg tgccgcaacg gatcgtcttc ctcgacgcct
    15901 taccgctgac ggctaatggg aaaattgact accaggcgct gaagcgtcgt catacccctg
    15961 aagcggaaaa cccggccgaa gcggatttac cccagggcga cattgaaaaa caggttgccg
    16021 ccctctggca gcaactctta tcaactggca atgtcaccag agaaaccgac ttcttccagc
    16081 aaggcggcga tagcctgctg gcgacccgtc tgaccgggca acttcatcag gcaggttatg
    16141 aagcgcaatt aagcgacctg tttaatcatc cccggctggc ggattttgcc gccacgctgc
    16201 ggaaaaccga cgtcccggtc gaacaaccat tcgtccactc ccctgaagat cgctaccagc
    16261 cctttgcgct taccgacgtg cagcaggctt acctggtggg gcgtcagccg ggctttgccc
    16321 tgggcggcgt cggctcacat ttctttgttg aatttgaaat tgccgatctg gacctcaccc
    16381 ggctggagac ggtctggaac cgattaatcg cccgccacga tatgctgcgc gccatcgtgc
    16441 gtgatggaca gcaacaggtg ctcgaacaga cgcccccctg ggtgataccc gcacacaccc
    16501 tccatacgcc tgaagaggcg ttgcgggtgc gcgaaaaact ggcgcatcag gtactcaacc
    16561 ccgaagtgtg gccggtattc gatctccagg tcggatacgt ggacgggatg cctgcccgcc
    16621 tgtggctgtg tctggataac ctgttgcttg acggtctgag catgcagatc ctgctggcgg
    16681 agctggagca cggctaccgc tacccgcaac agctgcttcc gccgctgccc gtcaccttca
    16741 gggattatct gcaacaaccc tcgctacagt cgcccaatcc agattctctg gcatggtggc
    16801 aggcgcagct tgatgatatt cctccggcgc ctgcgttgcc gctgcgctgc ttgcctcagg
    16861 aggttgaaac accgcgcttc gcccgcctga acggcgcgct ggacagcacg cgctggcatc
    16921 ggctgaaaaa acgggcggct gacgcccatc tcaccccgtc ggccgtactg ttgtcggtgt
    16981 ggtcaacggt tctctctgca tggagtgcac agcctgagtt cacgcttaac cttacgcttt
    17041 tcgacaggcg accgctgcac ccgcaaatca accagattct gggcgatttc acctcgctga
    17101 tgctgctgag ctggcatccc ggcgaaagct ggctgcacag cgcgcagtca ctacagcagc
    17161 ggctgagcca gaacctcaac caccgagatg tgtcagccat ccgcgtgatg cgtcaactgg
    17221 cgcaacagca aaacgtgcct gccgttccga tgcccgtcgt ctttaccagc gcgctgggct
    17281 ttgagcagga taacttcctc gcccggcgta atctgctcaa accggtctgg ggcatctccc
    17341 agacgccgca ggtctggctc gatcaccaga tttatgaatc cgaaggcgaa ctgcgcttta
    17401 actgggattt tgtcgccgcg ctgtttcctg ccgggcaggt ggagcgccag tttgaacagt
    17461 attgcgcatt gctaaaccga atggccgagg atgaaagcgg ctggcaactg ccgctcgccg
    17521 cgctggtgcc tcccgttaaa caggcagggc aatgcgcaga gcgctcaccg cgcgtatgcc
    17581 ctgagcaatc tcagccacac attgcggcgg acgagagcac cgtcagcctg atttgcgacg
    17641 ccttccgcga ggtggttggc gagtctgtca cgcccgcaga aaacttcttt gaggcgggcg
    17701 caacgtcgct gaatctggtg caactgcacg ttttgttaca acgtcacgaa ttttccaccc
    17761 tgacgttgct tgacctcttc acccaccctt ctcctgctgc cctggccgat tatctggccg
    17821 gcgtcgccac ggtggagaaa acaaaacgac ctcgccctgt tcgccgtcgt cagcggcgga
    17881 tatagcgcga agcaaactga ttttccccgg aacgccatcg cgaacgcatg gcgttccatt
    17941 gactttgtga atcttaggaa acgggaccga ttatggataa cttgcgcttc tcttctgcgc
    18001 cgacagcaga ttccattgat gcatcgatcg ctcaacacta cccggactgc gaacctgtcg
    18061 cggttatcgg ctacgcctgc cattttcctg aatcgccgga tggcgaaacg ttctggcaaa
    18121 atctgctgga aggtcgtgaa tgcagccgac gctttacgcg cgaagtgctt ctggccgtcg
    18181 gtctggatgc cgccatcatt gacgatcctc attatgtcaa tatcggtacg gtgttagaca
    18241 acgccgactg cttcgacgcc accctgtttg gctattcgcg acaggaagcg gagtcgatgg
    18301 acccgcagca gcgcctattt ttgcaggcgg tctggcatgc gctggaacat gccggttatg
    18361 cccccggcgc cgtcccccat aagaccggcg ttttcgcctc ttcccggatg agtacctacc
    18421 ccggtcgcga agcattgaac gtgacagaag tcgcgcaggt aaaaggtctg caatctctga
    18481 tgggcaatga taaagactat attgccaccc gcgccgcgta caaactcaac ctgcacggcc
    18541 cggcgttatc ggtacagacc gcctgctcca gctcgctggt tgccgtgcat ctggcctgtg
    18601 aaagcctgcg cgcaggcgaa tccgatatgg cggttgccgg cggcgtggcg ctctctttcc
    18661 cccagcaggc aggctaccgc taccagcccg gaatgatttt ctctcctgat ggtcactgtc
    18721 gtccctttga cgcctcggct gagggcacct gggccggtaa cggtctcggc tgcgtggtgc
    18781 tgcgtcgcct gagagacgcg ctgctgtcag gcgatccgat tatctcggtg atcctctcca
    18841 gcgcggtcaa caacgacggc aacagaaagg tcggctatac cgccccttcc gtcgcagggc
    18901 aacaggcagt catcgaagag gcgttaatgc tggcggccat cgacgacagg caggtaggtt
    18961 acattgaaac ccacggcacc ggcacaccgc tgggcgacgc gattgaaatt gaagcgttac
    19021 gcaacgtcta tgcgcctcgc ccgcaggatc agcgctgtgc gctcggttcc gtgaaaagta
    19081 acatgggcca tctggatacc gcggcgggca ttgccggact gctgaaaacc gttctggcag
    19141 tcagtcgcgg gcaaattcct cccttactga attttcacac ccccaacccg gcgctgaaac
    19201 ttgaagagag cccctttacc ataccggtgt cggcacaggc atggcaggac gaaatgcgct
    19261 atgcgggcgt ctcctccttt ggtattggcg gcaccaactg ccatatgatc gtcgcctcgc
    19321 tgcccgacgc gctcaacgcg cgcctcccca atacggatag cggcagaaaa agtaccgcgc
    19381 tgctgctcag cgccgccagc gacagcgcgt tgcggcggct ggcgacggat tatgccgggg
    19441 cgctgagaga gaatgcggat gccagctctc tggccttcac agccctgcac gcgcgccgtc
    19501 tcgatctccc cttccgcctg gcggcgccat taaaccgtga aaccgccgag gcgctcagcg
    19561 cctgggccgg tgagaaatcg ggggcgctgg tttacagcgg ccacggcgcc agcggcaagc
    19621 aggtgtggct gtttaccggc cagggctcgc actggcgcac tatgggtcaa acgatgtacc
    19681 agcactcaac ggcgtttgcc gacacgctgg atcgctgttt ttccgcctgt agcgaaatgc
    19741 tcacgccgtc actgcgcgaa gcgatgttta accccgattc ggcgcaactg gacaatatgg
    19801 cctgggcgca gccggcgatt gtcgcgtttg aaatcgcgat ggcggcgcac tggcgtgctg
    19861 aaggactgaa gccagacttc gccattgggc attccgtcgg tgaatttgcc gctgctgttg
    19921 tctgcggaca ctatacgatt gaacaggtca tgccactggt ttgtcggcgc ggcgcgctaa
    19981 tgcagcagtg cgcaagcggc gcgatggtgg cggtatttgc agacgaagac acgctgatgc
    20041 cgctggctcg ccagtttgag ctggatctcg ccgccaataa cggtacgcaa catacggtat
    20101 tttccgggcc ggaagcccgt ctcgcagtat tttgcgccac gctctcgcag catgacatta
    20161 actatcgtcg cctgagcgta accggtgcgg cgcactccgc tttactggag ccgatactcg
    20221 atcggttcca agacgcctgc gcgggactgc acgcggagcc ggggcaaata ccgattattt
    20281 ccacgctcac cgccgacgtc attgatgagt caacgctcaa ccaggcggat tactggcgcc
    20341 gacacatgcg ccagccggtg cgttttatcc agagtattca ggtggcgcat cagctcggcg
    20401 cccgcgtttt tctggagatg gggcccgatg cccagttggt tgcttgcggg cagcgcgaat
    20461 accgcgataa cgcatactgg atagccagcg cccggcgtaa caaagaggcg agcgatgtcc
    20521 tcaatcaggc cctgctccag ctttacgctg ccggcgtcgc cctaccgtgg gccgacctgc
    20581 tggcgggcga tggacaacgt atcgctgcgc catgttatcc gtttgatact gagcgttact
    20641 ggaaagagcg cgtctccccg gcctgcgagc ctgccgacgc agcgctgtct gccgggctgg
    20701 aggtggcgag tcgcgccgcg acagcgctcg atctccctcg cctggaagcg cttaaacagt
    20761 gcgccacgcg actgcacgcc atctacgtcg atcaactggt acaacgctgt accggcgatg
    20821 ccattgagaa cggcgtggac gccatgacca tcatgcgccg tggacgtctg ctgccccgct
    20881 accagcagct actccagcgc ctgctgaata actgcgtggt cgacggcgat taccgctgca
    20941 ccgacgggcg atacgtccgc gcccgcccca ttgaacatca acagcgggaa tcactgctga
    21001 cggaacttgc cggttattgt gaaggttttc aggctattcc cgacaccatc gcccgtgccg
    21061 gcgatcggtt atatgaaatg atgagcggcg cggaagaacc ggtggcgatt atcttcccgc
    21121 aaagcgcctc cgacggcgtg gaagtgctgt atcaggaatt cagctttggc cgctatttca
    21181 accaaatcgc cgccggggta ttacgcggca ttgtccagac gcgtcagccc cgccagccgt
    21241 tgcgtattct tgaagttggc ggcggaaccg gcggcaccac cgcgtggctg ctgccggaac
    21301 tcaacggcgt tccggcactg gagtaccatt tcaccgatat ctcggcgctg ttcacccgtc
    21361 gcgcccagca gaaattcgcc gactatgatt ttgtgaagta tagcgagctg gatctcgaaa
    21421 aagaggcgca gtctcagggt ttccaggcac agtcttacga tcttatcgtg gcagcgaacg
    21481 tgattcacgc cacccgccat attggccgca cgctcgataa tctgcgcccc ctgctcaagc
    21541 cgggcgggcg cctgctgatg cgcgaaatca cccagccaat gcgtctgttt gacttcgttt
    21601 tcggcccgct ggttcttccg ctacaggatc tcgacgcccg cgaaggtgag ttattcctca
    21661 ccaccgctca gtggcaacaa cagtgccgcc acgccggatt cagcaaagtg gcgtggctac
    21721 cgcaggatgg cagcccgacc gccgggatga gcgaacatat cattctcgcc acgctgcccg
    21781 gtcaggcggt tagcgccgta acattcaccg cgccatcaga acccgtgttg gggcaggcgc
    21841 tgacggataa cggtgattat ctcgccgact ggtctgattg cgcaggtcag cccgaacggt
    21901 ttaacgcccg ctggcaggag gcctggcgtc tgctttcaca gcgtcatggc gacgctctgc
    21961 ctgtggaacc gccccccgtc gccgccccgg agtggctggg gaaggttcgc ttaagctggc
    22021 aaaacgaagc cttttcccgc ggtcagatgc gcgttgaagc ccgtcatcct gctggcgagt
    22081 ggctgccgct atcgcccgcc gcgcctcttc ctgcgccgca gacgcattat caatggcgct
    22141 ggacgcccct caacgtcgcc agcattgacc aaccgcttac ctttagcttc agcgccggta
    22201 cgcttgcgcg cagcgacgag ctggcgcaat acggcatcat tcacgatccg cacgcctctt
    22261 cgcgactgat gattgttgag gagagcgagg atacgctggc cttagcggag aaagtgatag
    22321 cagcgctcac cgccagcgca gccggattga ttgtggttac tcgccgcgcg tggcgagtcg
    22381 aggaaaatga agcactctct gcatcccatc acgcgctatg ggccttgctt cgcgtcgcgg
    22441 ccaacgaaca gccggaacgg ttgcttgccg ccatcgatct cgccgaaaac cccccgtggg
    22501 aaacgctgca tcaagggttg agcgcagtct cactatcaca gcgctggctc gccgcacggg
    22561 gtgacaccct ttggctccct tcactggcgc ccaatacggg atgcgccgct gaattaccgg
    22621 caaacgtgtt taccggcgat agccgctggc atctggtgac cggagcgttt ggcggattag
    22681 gccgccttgc cgtgaactgg ctcagagaaa aaggggcgcg acgcatcgcc ctgctggcgc
    22741 cgcgcgtgga tgagtcatgg ctacgcgacg tggagggcgg gcagacgcgc gtctgccgtt
    22801 gtgatgtggg cgatgccggg caactggcca cggttcttga cgatctggcg gccaacggcg
    22861 gcattgccgg agcgattcat gccgctggcg tattggctga cgcgcccttg caggagcttg
    22921 atgaccacca gctggctgcc gttttcgcgg taaaagcgca ggcggcaagc cagctgttgc
    22981 aaaccctgcg caaccacgac ggacgctatc ttattctcta ctcttccgct gccgccaccc
    23041 tcggcgcgcc gggtcagagc gcccatgcgc tggcctgcgg ctacctggac gggctggccc
    23101 agcagttttc cacccttgat gcgccgaaaa cgctctctgt cgcctggggc gcatggggag
    23161 aaagcggtcg ggcggccacg ccggaaatgc tggcgacgct cgccagccga ggtatgggcg
    23221 cgttaagcga tgccgaaggc tgctggcacc tggaacaggc ggtgatgcgc ggcgccccgt
    23281 ggcgactggc gatgcgcgtt tttaccgaca aaatgccccc gttacaacag gctctgttta
    23341 acatcagcgc cacagaaaaa gccgcaacgc cggtcattcc tcctgctgat gacaacgcct
    23401 ttaacggcag cctgagcgat gaaacagcgg tgatggcatg gctgaaaaag cggattgcgg
    23461 ttcagctaag gctgagcgat ccggcgtcac tgcatccaaa ccaggatctg ttgcaactcg
    23521 gcatggactc gctgctcttc cttgaactca gtagcgatat tcagcactac ctgggcgtac
    23581 gcatcaatgc ggaacgggcg tggcaggatc tgtctcctca tggactcacg cagcttatct
    23641 gttctaagcc agaggcgacg cctgccgctt cgcagccgga agtgttgcgg cacgacgccg
    23701 acgagcgtta tgcgcccttc cctttgacgc ccattcagca cgcctactgg ctggggcgaa
    23761 cccacctcat tggctatggc ggcgtcgcct gtcacgtcct gtttgagtgg gataaacgcc
    23821 acgatgagtt caatctcgcc atactggaga aagcatggaa ccagctcatc gcacgccacg
    23881 atatgttgcg tatggtggtt gatgccgacg ggcagcagcg aatcctggcg acaacgccgg
    23941 agtatcacat cccgcgtgac gatctgcgcg cgctttcccc ggaagaacag cgcatcgcgc
    24001 tggaaaaacg gcggcatgaa ctgagctatc gcgttttgcc tgccgaccag tggcctcttt
    24061 ttgagctggt ggtcagcgaa atcgacgatt gccattaccg tctgcatatg aacctcgacc
    24121 ttttgcagtt tgatgtgcag agttttaaag tcatgatgga cgacctggcg caggtctggc
    24181 gcggtgaaac gctggcgccg ctcgctatta ccttccgtga ttatgtgatg gctgaacagg
    24241 cgcgccgaca gacatcggca tggcacgatg cctgggatta ctggcaggaa aaactgccgc
    24301 aactgccctt agcgccagag ctgccggtgg ttgagacgcc cccggaaacg ccacacttca
    24361 ccaccttcaa atcgacgatc ggcaagacag aatggcaggc cgtgaaacag cgctggcagc
    24421 agcaaggcgt cacaccgtct gccgcgctgc tcacgctgtt tgcagccacc cttgagcgct
    24481 ggagccgtac cacaacattt acgctgaacc tgacgttctt caatcgccag ccgatccatc
    24541 cgcaaatcaa ccagttgatt ggtgatttta cctccgtcac gctggttgat tttaacttct
    24601 cagcgccggt gacgttgcaa gagcagatgc aacagaccca acagcgcctc tggcaaaaca
    24661 tggcgcacag tgaaatgaac ggtgttgagg tgatccgtga gctgggccgc ctgcgcggat
    24721 cacaacgtca accgctgatg ccggtagtgt ttaccagtat gctggggatg acgctggaag
    24781 gcatgactat cgatcaggcg atgagccatc tgttcggcga accctgctat gtattcacgc
    24841 aaacgccgca ggtctggctg gatcatcagg tcatggagag cgacggcgag ttgatgttta
    24901 gctggtactg catggacaac gtgctggaac ccggcgctgc cgaggcgatg tttaatgact
    24961 attgcgccat cctgcaagcc gtcatcgccg cccctgaaag cctgaagact ctcgccagcg
    25021 gcatcgccgg gcacattccc cgccgacgct ggccgctgaa cgcgcaggcg gactacgacc
    25081 tgcgggatat tgagcaggcg acgctcgaat accccggcat ccggcaggcc agagcggaaa
    25141 taaccgaaca gggcgcgttg acgctggata tcgtgatggc cgacgatccg tcgccatcag
    25201 cggcgatgcc tgatgagcac gaacttaccc aactggcgct gccgttgcct gagcaggcgc
    25261 agcttgatga gctggaggcg acctggcgct ggctggaggc gcgtgcgcta caggggatcg
    25321 cggctacgct aaatcgtcac ggcctgttta ccacgccgga gatcgcccat cgctttagcg
    25381 caatagtaca ggcgctgtcc gcgcaagcgt ctcaccagcg tctgctgcgc cagtggctac
    25441 agtgtctgac ggaaagagag tggttaatcc gcgaaggtga aagctggcgc tgccgcattc
    25501 cgctcagcga gattcctgag cctcaggaag cgtgcccgca aagccaatgg agccaggcgc
    25561 tggcgcagta tctggaaacc tgcatcgccc ggcacgacgc cctcttctcc gggcggtgtt
    25621 ctccgctgga attgctgttc aacgagcagc atcgcgttac cgacgcgctg tatcgcgaca
    25681 accccgccag ctcctgtctg aatcgctata ccgcgcagat tgccgccttg tgcagcgcag
    25741 aacggattct ggaggttggc gccggaaccg cagccactac cgcgccggtg ctgaaggcca
    25801 cgcggaacac gcggcagtcg taccacttca cggacgtctc cgcgcagttc ctcaatgacg
    25861 ccagagcccg tttccatgat gaatcgcagg tgtcttatgc cttgttcgac atcaaccagc
    25921 cgctggattt caccgcccac ccggaggcgg gttacgacct gatcgttgcc gtcaatgtgc
    25981 tccacgacgc cagccatgtc gtccagacgt tgcgcagatt aaaactgttg ctgaaagccg
    26041 gcggacgttt gctgatcgtt gaagcgacgg agcgaaacag cgtattccag ctggcgagcg
    26101 tgggctttat tgagggatta agcggatacc gcgatttccg ccgccgggat gagaaaccga
    26161 tgctcacccg ctccgcatgg caggaggttc tcgttcaggc cgggtttgca aacgagctgg
    26221 cgtggcccgc gcaggaatcg tcgccgctgc gccagcatct gctggtggcg cgttcgcctg
    26281 gcgtaaatcg cccggataaa aaagccgtga gccgctattt acagcagcgc tttggcaccg
    26341 gtctgcccat tttacagatc cggcaaagag aagcgttatt tacgccgctg catgccccgt
    26401 ctgatacgcc gactgagcca gccaaaccca cgccagttgc cggggggaat ccggcgctgg
    26461 aaaaacaggt ggctgaactc tggcaatcgc tgctgtctcg ccccgtggca aggcatcacg
    26521 actttttcga actgggcggc gacagcctga tggcgacaag gatggtcgcg cagctaaacc
    26581 ggagagggat tgccagggct aaccttcagg atctgttcag ccattcgacg ctgagcgact
    26641 tctgcgccca tctacaggcg gctacgtcag gagaggacaa cccgataccc ctttgccagg
    26701 gcgacggtga ggaaaccctg tttgtcttcc acgcttcaga cggcgatatc agcgcctggc
    26761 tgccgctcgc tagcgcgttg aacaggcgcg ttttcggcct gcaagcaaaa tcgccgcagc
    26821 gctttgccac gctcgaccag atgatcgatg agtatgtcgg gtgcatccgt cgtcagcagc
    26881 ctcacggccc ttatgtgctg gcgggttggt cgtatggcgc gtttcttgcg gcgggcgccg
    26941 cacagcgcct gtacgccaaa ggcgagcagg ttcggatggt gttaatcgat cccgtgtgcc
    27001 gacaggattt ctgttgcgaa aaccgggcgg ccctgctgcg cctgttagcc gaaggacaaa
    27061 cgcctctggc actgcccgaa catttcgacc agcagacgcc cgacagccag cttgccgact
    27121 ttatcagcct cgctaaaacg gccggtatgg tgtcgcaaaa cctgacgctg caagcggcag
    27181 aaacgtggct cgacaacatc gcgcatctgc tgcgtttact gactgagcat acgccgggcg
    27241 aaagcgttcc ggtcccctgt ctcatggtgt atgccgccgg gagacccgcg cgctggacgc
    27301 cagcagaaac cgagtggcag ggctggataa acaacgccga cgacgctgtg attgaagcca
    27361 gccactggca aatcatgatg gaagcccccc acgttcaggc ttgtgcgcaa cacattacgc
    27421 gctggctttg cgcaacctca atgcaaccgg agaacacgtt atgatgccgt ccgcctcccc
    27481 aaaacaacgc gtactgattg tgggcgccaa atttggcgaa atgtacctga atgcctttat
    27541 gcagcccccg gaggggctgg aactggtcgg cctgctggcg cagggaagcg cccgttcaag
    27601 agagctggct catgcgtttg gcattccgct gtatacctcg ccggaacaga taaccaagat
    27661 gccggatatc gcctgtattg tcgtgcgttc gaccgtcgcc ggaggaaccg gtacgcagct
    27721 tgccagacac tttctgacgc gcggcgtaca cgtcattcag gagcatcccc tccacccgga
    27781 tgacatcagt tccttacaaa cgctggcgca ggaacagggc tgctgctatt gggtaaacac
    27841 gttttatccg catacgcgcg cgggacgcac atggcttcgc gatgctcagc agctgcgtcg
    27901 ctgcctggct aagacgccgc cggtagtcca cgccaccacc agccgacagt tgctctactc
    27961 cactctggat ttgttgttgc tggctctggg cgttgatgcc gccgccgtgg agtgcgacgt
    28021 ggttggcagc tttagcgatt ttcactgtct gagactcttc tggccggagg gagaagcctg
    28081 cctgctgctt cagcgctatc tcgatcctga cgatccagat atgcatagcc ttatcatgca
    28141 ccgcctgctg ctgggctggc cggaaggtca tctttcgctg gaggcgagct acggccccgt
    28201 catctggtca tccagcctgt ttgtcgcaga ccatcaggag aacgcccaca gcctctaccg
    28261 cagaccggag atcctgcgcg atctgccggg tctgacgcgc agcgcggcgc ccctgagctg
    28321 gcgcgactgc tgcgaaaccg tcgggccgga gggcgtcagc tggttgctac accagttgcg
    28381 cagccatctg gcgggcgaac atccaccagc ggcctgccaa agcgtacatc agatcgcgtt
    28441 atcacgttta tggcagcaga ttttacgtaa gaccggcaat gcggagatca gacgcctgac
    28501 accgccgcac cacgaccggt tagccggttt ctacaacgat gatgataagg aggcgctgtg
    28561 acgcaatctg caatatgcat cccgctgtgg cccgcccgga acggcaatac tgcgcatctg
    28621 gtcatgtgcc ctttcgctgg cggcagcagt agcgcgtttc gccactggca agctgagcaa
    28681 ctggctgatt gcgcgctttc tctggtgacc tggccggggc gcgatcgcct tcgccatctg
    28741 gaaccgctca gaagcattac acaactggcg gcactgctgg cgaacgagct ggaagcatcc
    28801 gtatcgcctg acacgccgct tttactcgcc gggcacagca tgggggcgca ggtggcgttt
    28861 gaaacctgcc gacttctgga gcaacggggg cttgcgccac aaggactgat tatttccggg
    28921 tgccatgccc cgcatctgca ttctgaacgc cagctcagcc atcgcgatga tgccgacttt
    28981 atcgctgagc tgatagacat tggcggatgt tctcctgaac tgcgggaaaa ccaggaatta
    29041 atgtcgctgt ttcttcctct tctgcgcgct gatttttacg ccaccgagag ctatcactac
    29101 gactcgcccg acgtctgtcc gccgctgcgc acgcctgcgc tgttattgtg cggcagccac
    29161 gatcgcgaag cctcctggca gcaggtcgat gcctggcgtc agtggctgag ccacgttaca
    29221 ggcccggtgg tgattgacgg cgatcatttc tatcccattc aacaagcccg gtcctttttt
    29281 acgcagattg tccgccattt tccccacgca ttttctgcaa tgaccgcgtt gcaaaaacag
    29341 cccagtactt cagaaaggtg acgcatgaat tcttccttta aatctctgat tgaacagtat
    29401 cccttaccca ttgccgaaca gttgcgccac tgggcggccc gttatgcctc gcgaattgcc
    29461 gtcgttgatg caaaggggtc gttaacctac agcgcgcttg atgcacaagt tgacgaactt
    29521 gccgcaggtc tgtcatcact gggtttgcgt tcgggggagc atgtaattgt gcagcttccc
    29581 aacgacaacg cgtttgttac cctgctgttc gccttgttaa gagtgggcgt tatccccgtg
    29641 ctggcgatgc cctcgcaacg ggcgctggat atcgacgcgc tgattgagct ggcgcaaccc
    29701 gtcgcttacg ttattcacgg ggaaaaccac gcagagctgg cccgacagat ggcgcacaaa
    29761 cacgcctgct tgcgtcatgt tctggtcgct ggagagaccg tgagcgacga ttttacgccg
    29821 ttcttctccc ttcacggtga gcgacaggca tggccgcagc ctgatgtttc caccaccgcg
    29881 ttgttgttgc tctcaggcgg cacaaccggc acgcccaaac tcatcccgcg ccgacatgcc
    29941 gactatagct ataacttcag cgcttctgct gaactgtgcg gcatcagcca acagagcgtg
    30001 tatctcgccg tcctcccggt ggcgcataac tttccgctgg cctgccccgg tattctggga
    30061 acgcttgcct gcggcggaaa agtggtgctg accgacagcg ccagctgtga tgaggtgatg
    30121 cctttaatcg cgcaggaaag agtgactcac gtcgccctgg ttccggcgct ggcgcaatta
    30181 tgggtgcagg ccagggagtg ggaagacagc gacctttcgt cgctgcgcgt cattcaggca
    30241 ggcggcgccc ggctcgaccc gacgcttgct gagcaggtta tcgccacctt tgactgtacc
    30301 ctgcaacagg ttttcggtat ggcggaaggc ctgctctgtt ttacccggct ggacgatccg
    30361 catgccacca ttctccacag ccaggggcgc ccgttgtccc ctctggatga aatccgcatc
    30421 gttgatcaag acgagaacga cgtcgcgccg ggcgaaaccg ggcaattgtt aacgcgcggc
    30481 ccttatacca tttcgggcta ttaccgcgcc cctgcccaca acgcgcaggc ctttaccgcg
    30541 caagggtttt accgcacagg cgacaatgtc aggctggatg aggtggggaa cctgcacgtt
    30601 gagggacgca taaaagagca gatcaaccgc gccggagaaa aaatagccgc ggctgaagtg
    30661 gaatcggcac tgctgcgttt agcggaagtg caagattgcg cggtggtcgc cgcgccggac
    30721 acgctgcttg gcgagcggat ttgcgcgttt atcatcgcgc agcaggtgcc aactgactat
    30781 cagcagttgc gtcaacaact gacccgtatg gggctcagcg cgtggaaaat tcctgaccaa
    30841 atcgagtttc tggaccactg gccgctcacc gccgtcggca agatagacaa aaaacgcctg
    30901 acggctctcg ccgtcgaccg ttatcgccat tctgcccaat aagcgcaaac cgacccgaaa
    30961 caggttgaaa taaacccgtt tcgggtagca ccactattag aaatagttat cattttcaat
    31021 tcaccattgt cggtattttt ggcgtttcgc cgtcttacag ggactcacaa caatgaaaat
    31081 gacacggctt tatcctctgg ccttgggggg attattgctc cccgccattg ctaatgccca
    31141 gacttcacag caagacgaaa gcacgctggt ggttaccgcc agtaaacaat cttcccgctc
    31201 ggcatcagcc aacaacgtct cgtctactgt tgtcagcgcg ccggaattaa gcgacgccgg
    31261 cgtcaccgcc agcgacaaac tccccagagt cttgcccggg ctcaatattg aaaatagcgg
    31321 caacatgctt ttttcgacga tctcgctacg cggcgtctct tcagcgcagg acttctataa
    31381 ccccgccgtc accctgtatg tcgatggcgt ccctcagctt tccaccaaca ccatccaggc
    31441 gcttaccgat gtgcaaagcg tggagttgct gcgaggccca cagggaacgt tatatggcaa
    31501 aagcgctcag ggcgggatca tcaacatcgt cacccagcag ccggacagca cgccgcgcgg
    31561 ctatattgaa ggcggcgtca gtagccgcga cagttatcga agtaagttca acctgagcgg
    31621 ccccattcag gatggcctgc tgtacggcag cgtcaccctg ttacgccagg ttgatgacgg
    31681 cgacatgatt aaccccgcga cgggaagcga tgacttaggc ggcacccgcg ccagcatagg
    31741 gaatgtgaaa ctgcgtctgg cgccggacga tcagccctgg gaaatgggct ttgccgcctc
    31801 acgcgaatgt acccgcgcca cccaggatgc ctatgtggga tggaatgata ttaagggccg
    31861 taagctgtcg atcagcgatg gttcaccaga cccgtacatg cggcgctgca ctgacagcca
    31921 aaccctgagt gggaaataca ccaccgatga ctgggttttc aacctgatca gcgcctggca
    31981 gcagcagcat tattcgcgca ccttcccttc cggttcgtta atcgtcaata tgcctcagcg
    32041 ctggaatcag gatgtgcagg agctgcgcgc cgcaaccctg ggcgatgcgc gtaccgttga
    32101 tatggtgttt gggctgtacc ggcagaacac ccgcgagaag ttaaattcag cctacgacat
    32161 gccgacaatg ccttatttaa gcagtaccgg ctataccacc gctgaaacgc tggccgcata
    32221 cagtgacctg acctggcatt taaccgatcg ttttgatatc ggcggcggcg tgcgcttctc
    32281 gcatgataaa tccagtacac aatatcacgg cagcatgctc ggcaacccgt ttggcgacca
    32341 gggtaagagc aatgacgatc aggtgctcgg gcagctatcc gcaggctata tgctgaccga
    32401 tgactggaga gtgtataccc gtgtagccca gggatataaa ccttccgggt acaacatcgt
    32461 gcctactgcg ggtcttgatg ccaaaccgtt cgtcgccgag aaatccatca actatgaact
    32521 tggcacccgc tacgaaaccg ctgacgtcac gctgcaagcc gcgacgtttt atacccacac
    32581 caaagacatg cagctttact ctggcccggt cgggatgcag acattaagca atgcgggtaa
    32641 agccgacgcc accggcgttg agcttgaagc gaagtggcgg tttgcgccag gctggtcatg
    32701 ggatatcaat ggcaacgtga tccgttccga attcaccaat gacagtgagt tgtatcacgg
    32761 taaccgggtg ccgttcgtac cacgttatgg cgcgggaagc agcgtgaacg gcgtgattga
    32821 tacgcgctat ggcgcactga tgccccgact ggcggttaat ctggtcgggc cgcattattt
    32881 cgatggcgac aaccagttgc ggcaaggcac ctatgccacc atggacagca gcctgggctg
    32941 gcaggcgact gaacggatga acatttccgt ctatgtcgat aacctgttcg accgtcgtta
    33001 ccgtacctat ggctacatga acggcagcag cgccgtcgcg caggtcaata tgggtcgcac
    33061 cgtcggtatc aatacgcgaa ttgatttctt ctgattattg taaaagggat accgaaaagg
    33121 tatccctttt acaccactag ttaaaaccag taactcagca gagtcgcaaa aaatattaat
    33181 ccatagtgat tatttaaaca atgaaattgc gattaggata aatagattta actttctcgt
    33241 tcctttctct ccttatacta aagaaataat catatcaaaa taaaaattca caacagtgca
    33301 acattaaaaa atacaaccaa caaacaatcc tatatacaag gcacatctcc agaatataaa
    33361 agcacagaca aacaacctaa aaaacaaccc catacatata aattatttta taggtaaaat
    33421 agattatata ttctcatagc taccataaac tatactagcc tgaactatat ttattctgct
    33481 gcaatcaatg catacataac acaaatatca ctcaggtaca ctactcaaac cacgctggga
    33541 tttttcctca agttataatc atcaccccga aaatcattcg gcatttactc attaaatagt
    33601 caccccatag gcctgtacat gtttactcag aaatatacat ccttttctct gtcataaacc
    33661 ctctgattaa tcataaataa atacttgtga caccaatctt tttccttaac ggaacgaatt
    33721 gttgtgtaga aggagataat attatgtcaa aaaaatatca gccattgctt ataactcatt
    33781 atatgtcaac atgggtcact ataacggaag cagttgagat cactaccaaa gccataaaac
    33841 aaaaaattac tcctagcgat atttatcgtc atgccttgag tggcaacatc ctactattgg
    33901 tctatttcca gtctcctgtg atacttaaga agatacaaac ttttaatgga aaaataaaat
    33961 tcaggcagtt tgtgggagac ctacttgata aactatgcat gcttgacagg gacggtttta
    34021 tttatggtca aaatttaaga ctctgtacag aagccagata tatttgtcct gtgcaacaaa
    34081 ttatagatac ccccctgtta cgcaaactca accagtttcg tacgtttgtt cgtaatgtcc
    34141 gcccgggtga tgaactggat gtccaggcac aagttagtga aaaaaattta accccgccgc
    34201 cgggtaacag cagtggcaac cttgagcaac agatagccag tacttcacag ctaattggat
    34261 ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa catagcacgt ggatgggcct
    34321 cttctcaggc ttcaggggta atgacagact ggttaagccg cttcggtacc gcaagaatca
    34381 cgctgggcgt ggatgaagat ttcagcctga agaactcccg tgacggcaac cctcgaaaat
    34441 ggcgattcga tgcaacaaac agtgaactat gtgccgaacg tcacgaatgc tgaaatcacg
    34501 ctggcagcct tgaaggatcc ggtgattgcc gacaataacg atctcacgac actaacagca
    34561 ccgtcgctga tacagagggc aatgcgatag ccaacactga ggtaacattt actctaccgg
    34621 aagatgtgaa ggcgaacttc acgctgagcg atggcgacaa agcggttact gatgccgatg
    34681 gcaaagcgaa agtcacgctg aaaggtacaa aagcaggcgc tcataccgtg acagcatcga
    34741 tggttggcgg taagagtgag cagttagtgg tgaactttac tgcggatacg ctcactgcgc
    34801 aggttaatct taacgttacc gaggacaatt tcatcgccaa taacattgga atgaccaggc
    34861 tacaagcaat agtgactgat ggaaacggca acccagtaga aggtataaaa gttaatttcc
    34921 gtggaacctc cgtcacgcta agcagcacca gcgttgaaac ggatgatcag gtttttgctg
    34981 aaattcttgt gacaagcacc gaggtcggac taaaaacagt ttcagcctct ctggcagata
    35041 aacctactga agtcatctcg cgattactga atgccaaggt agatgttaat tctgcgacga
    35101 ttaccagtca ggagatacct gaaggtcagg tcatggtcgc acaagacata gcagttaaag
    35161 ctcacgttaa cgaccagttt ggcaacccgg tgacgcatca agccgcgaca ttcagtgcag
    35221 cgccatcctc gcaaatgatc atcagccaga acacggtctc tactaatacg cagggagtag
    35281 ccgaggtcac catgacgtct gaaagaaacg gctcgtatac tgtaaaagca tccctagcga
    35341 atggagcttc acttgagaaa caactggagg ctattgatga aaaactgaca ctcacgtcca
    35401 gtccgcttat cggtgtcaat gctcctaaag gcgctactct gacggcaacg ctaacctctg
    35461 caaatggcac tccagtggag ggtcaggtca tcaactttag cgtaacgcta gaaggtgcga
    35521 cgttaagtgg cggaaaagtg agaaccaact cttcaggtca ggctccagtc gttctgacca
    35581 gcaataaagt cggtacatat acggtgactg catcgttcca taacggcgtc acaatacaga
    35641 cacagacaac cgtgaaggtt actggcaacc caagcaccgc acatgttgct agctttatcg
    35701 ctgatccgtc gactatcgcc gccaccaaca gtgatttaag taccttaaag gcaacggttg
    35761 aggatggcag tggtaacctg atcgaaggtc tcactgtgta cttcgcctta aaaagcggct
    35821 ccaccacatt aacgtcatta acagcggtga ccgatcaaaa cggaatcgcg acaacaaacg
    35881 tgagatgagc gataacaggg agcgtcactg taagcgcagt cataagcgca ggtggaatgc
    35941 aaacagtaga tatatcgcta gtggcaggcc cagcagacgc ctcgcagtcc atccttaaga
    36001 acaaccagtc atcactgaaa ggggacttta ccgatagtgc tgagctacat cttgttctgc
    36061 acgatatatc aggcaatccg atcaaagatt ctgaagggat ggaatttgtg caatcaggta
    36121 caaacgtgcc ctatatgaaa attagcgcaa ttgattacag tcaaaatatc aacggtgatt
    36181 acaaagctac tgttacagga gacggagagg gtatcgcaac gctgatcccc gtattgaatg
    36241 gtgttcatca ggcgggcctg agtaccacga tagaattcat tagcgcggaa accagaccaa
    36301 tgacgggtac agtgtcagtg aatagtgcaa acctaccgac agcttcattc ccttcgcagg
    36361 gatttaccgg ggcgtattat cagttgaata atgacaactt tgcaccagga aaaacggcgg
    36421 ctgattattc gttttcaagc tcggcctcct gggtaggcgt tgatgctact ggtaaagtga
    36481 cattcaaaaa tgacggtgat agcaatacag taataatcac agcgccccca agatccggtg
    36541 gtgctattta ccaaacagtt cctcctgaat cgcgttcggt gtgactatga tcattttcgg
    36601 tgagtattgc tttgatccaa aggctggttt tctgtgatga ttgaccagcc tcagagtatt
    36661 gatgacacca ccgttcataa cagatcggca ttactatgca taagtactgc ctcaaattct
    36721 cgccaaaaca ctttttaatg cctttgcgaa ttcatccaga cttatattgt caattttttg
    36781 cccaacgacg actgactccg aaacatcggc cattgcgggc catcgcgttt tgtaatcagc
    36841 ataaattgca acaacagggg tattataggc acttgccata tgtaatattg aggtgtcagg
    36901 agttattgca atataagcat cttttataat tgctgctgaa cgtttaatcg agggtgataa
    36961 ggataaacgg tagacattat tataacaatc cactaatgcc ctggctttat cttccccttt
    37021 gggtccataa acgatcacta ttggaatatc tgtttcactt tgaatttttg ctattagatt
    37081 ttctgcaatc gataatgaaa atgtacgttc ctgcgagcta ccgtctaaat tgagcgcaat
    37141 gtaagatcct aacgatcgca tttcctcgcg tacctcatcg agtacatcat cgcttaatgg
    37201 caattcatat attgacctga ctaccggaaa acccgcctca cgcatcataa atgcccatgt
    37261 catgggtaca ggagtccgga gattctgatc catccgggac gcgttcttac acaaagggga
    37321 atagcacttc atggttaaac cgacgacctg gaaattcgtt tttgctttca actgactgat
    37381 aaatatcatc gttttcaggt tctttttacg catcgcctct atacaaagat ccggcgtacc
    37441 gtattgcagt gttatattct tcgctaaatc ttttatttct tttaatgttg cgtgatcctg
    37501 catagtcatt gtgactaatg ttaatttagt ctgttcaagt ttgagcgcat taaagacttc
    37561 taaattaatt gtcgacgtaa caattaatag atgcttaatt ttatgcaatt caagcgcccg
    37621 aataacagga aagatggcca tagcatcgcc aatctgatcg ggaatatgta tgacaacaaa
    37681 gtctgttttt tcaatattaa aattataagc tttataatcg tagtaactaa atgcaatacg
    37741 tctcaacaat gatactaaaa acataactaa cctcgcctcc ctactggtta taatgcaatg
    37801 cagtctatca gaatcatcag ggtgcaattt tgtgcatatg cggactttta tgtttcatat
    37861 ctcgaacctg tgggccatct gcttaattct taaacaacac cagcaactcc tgcgctttca
    37921 tcttccatcg aatttttcat gttgccgcta atcagccata aaatcatttg cagatgcgct
    37981 ctgtcgaggt agtctcataa ggttcgttta tagatcgacg gcaatgtgag ttaccttttc
    38041 catactaatt ataaaaagac agtacaaaca ggatcattat ggactccacg ctcatctcca
    38101 ctcgtcccga tgaagggacg ctttcgttaa gtcgcgcccg acgagctgcg ttaggcagtt
    38161 tcgctggtgc cgtcgtcgac tggtatgatt ttttactcta tggcattacc gccgcactgg
    38221 tgtttaatcg cgagtttttc ccgcaagtaa gcccggcgat gggaacgctc gccgcatttg
    38281 ccacctttgg tgtcggcttc cttttccgac cgctcggcgg tgtcattttc ggtcactttg
    38341 gtgaccggct gggacgtaag cgcatgttaa tgctgaccgt ctggatgatg ggcatcgcga
    38401 cagccttgat tggtattctt ccttcattct cgaccattgg gtggtgggca cctattttgc
    38461 tggtgacact gcgtgccatt cagggatttg ccgtcggcgg cgaatgggga ggcgcagcgt
    38521 tgctttccgt tgaaagtgcg ccgaaaaata aaaaagcctt ttacagtagc ggtgtacaag
    38581 ttggctacgg agtaggttta ctgctttcaa ccggactggt ttcattgatc agtatgatga
    38641 cgactgacgt acagttttta agctggggct ggcgcattcc tttcctgttt agcatcgtac
    38701 tggtactagg agcattgtgg gtgcgcaatg gcatggagga gtccgcggaa tttgaacaac
    38761 agcaacataa tcaagcggcc gcgaaaaaac gcatcccggt tatcgaagct ctgttacgac
    38821 atcccggtgc tttcctgaag attattgcgc tacgactgtg cgagttgctg acgatgtaca
    38881 tcgttaccgc ctttgcactt aattattcaa cccagaatat ggggttaccg cgcgaacttt
    38941 tccttaatat tggtttgctg gtaggtggat taagctgcct gacaattccc tgttttgcct
    39001 ggcttgccga tcgttttggt cgccgcaggg tttatatcac aggtgcgtta atcggaatgt
    39061 tgagcgcatt tcctttcttt atggcgcttg aagcacaatc tattttctgg atagttttct
    39121 tctccataat gctggcaaac attgcgcatg acatggtggt gtgtgtgcaa caaccgatgt
    39181 ttaccgaaat gtttggtgcc agttatcgct atagtggcgc tggagtcggt tatcaggttg
    39241 ccagtgtggt tggtggtgga tttacacctt ttattgccgc tgcactcatc acttactttg
    39301 ccgggaactg gcatagcgtc gccatttatt tgctggctgg atgcctgatt tccgcaataa
    39361 ccgctttgtt gatgaaagac aatcaacgcg cttgatagcc tggcgaagat catccgatct
    39421 tcgccttaca cttttgtttc acattcctgt gacatactat cggatgtgcg gtaattgtat
    39481 ggaacaggag acacacatga ataataaggg ctccggtctg accccagctc aggcactgga
    39541 taaactcgac gcgctgtatg agcaatctgt agttgcttta cgcaacgcca ttggcaacta
    39601 tattacaagt ggcgaattac ctgatgaaaa cgcccgcaaa caaggtcttt ttgtctatcc
    39661 atcactgacc gtaacttggg atggtagcac aaccaatccc cctaaaacgc gcgcatttgg
    39721 tcgttttact cacgcaggca gctacaccac cacgattact cgccctactc tctttcgttc
    39781 gtatctcaat gaacaactta cgttgctgta tcaggattat ggtgcgcata tctcggtgca
    39841 accctcgcag catgaaatcc cttatcctta tgtcattgat ggctctgaat tgacacttga
    39901 tcgctcaatg agcgctgggt taactcgcta tttcccgaca acagaactgg cgcaaattgg
    39961 cgatgaaact gcagacggca tttatcatcc aactgaattc tccccgctat cgcattttga
    40021 tgcgcgccgc gtcgattttt ccctcgcgcg gttgcgccat tataccggta cgccagttga
    40081 acattttcag ccgttcgtct tgtttaccaa ctacacacgt tatgtggatg aattcgttcg
    40141 ttggggatgc agccagatcc tcgatcctga tagtccctac attgcccttt cttgtgctgg
    40201 cgggaactgg atcaccgccg aaaccgaagc gccagaagaa gccatttccg accttgcatg
    40261 gaaaaaacac cagatgcccg cgtggcattt aattaccgcc gatggtcagg gtattactct
    40321 ggtgaatatt ggcgtgggac cgtcaaatgc taaaaccatc tgcgatcatc tggcagtact
    40381 acgcccggat gtctggttga tgattggtca ctgtggtggc ttacgtgaaa gccaggccat
    40441 tggcgattat gtacttgcac atgcttattt acgcgatgat cacgttcttg atgcggttct
    40501 gccgcccgat attcctattc caagcattgc tgaagtgcaa cgtgcgcttt atgacgctac
    40561 caagttagtc agcggcaggc ccggtgagga agtcaaacag cggctgcgta caggtactgt
    40621 ggtaaccaca gatgacagga actgggaatt acgttactca gcttctgcac ttcgttttaa
    40681 cttaagccgg gccgtggcaa ttgatatgga aagtgcaacc attgccgcgc aaggatatcg
    40741 tttccgcgtg ccatacggga cactactgtg tgtttcagat aaaccgttgc atggcgagat
    40801 taaacttccc ggccaggcta accgttttta tgaaggcgct atttccgaac acctgcaaat
    40861 tggcattcgg gcaatcgatt tgctgcgcgc agaaggcgac cgactgcatt cacgtaaatt
    40921 acgaaccttt aatgagccgc cgttccgata ataagttctg aaccttctta tcaaaaatag
    40981 atgtgaagga ttaagtaaga catctggcaa atagcccgca ataaacgcgg gc
//
LOCUS       DAZGXZ010000026        40474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000026 DAZGXZ010000000
VERSION     DAZGXZ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.026"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..582)
                     /locus_tag="ACHKJF_001543"
     CDS             complement(<1..582)
                     /locus_tag="ACHKJF_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0948923.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQKITELIEVVYNRRVDR"
     gene            complement(842..2095)
                     /gene="amiC"
                     /locus_tag="ACHKJF_001544"
     CDS             complement(842..2095)
                     /gene="amiC"
                     /locus_tag="ACHKJF_001544"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP0948924.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            2327..3658
                     /gene="argA"
                     /locus_tag="ACHKJF_001545"
     CDS             2327..3658
                     /gene="argA"
                     /locus_tag="ACHKJF_001545"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP0948925.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3720..5546)
                     /gene="recD"
                     /locus_tag="ACHKJF_001546"
     CDS             complement(3720..5546)
                     /gene="recD"
                     /locus_tag="ACHKJF_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP0948926.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(5546..9088)
                     /gene="recB"
                     /locus_tag="ACHKJF_001547"
     CDS             complement(5546..9088)
                     /gene="recB"
                     /locus_tag="ACHKJF_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP0948927.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSVGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(9081..11969)
                     /gene="ptrA"
                     /locus_tag="ACHKJF_001548"
     CDS             complement(9081..11969)
                     /gene="ptrA"
                     /locus_tag="ACHKJF_001548"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP0948928.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12144..15512)
                     /gene="recC"
                     /locus_tag="ACHKJF_001549"
     CDS             complement(12144..15512)
                     /gene="recC"
                     /locus_tag="ACHKJF_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP0948929.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLDSSQELDAFVDVMPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLIPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15525..15848)
                     /gene="ppdC"
                     /locus_tag="ACHKJF_001550"
     CDS             complement(15525..15848)
                     /gene="ppdC"
                     /locus_tag="ACHKJF_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP0948930.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15833..16240)
                     /gene="ygdB"
                     /locus_tag="ACHKJF_001551"
     CDS             complement(15833..16240)
                     /gene="ygdB"
                     /locus_tag="ACHKJF_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP0948931.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16237..16800)
                     /gene="ppdB"
                     /locus_tag="ACHKJF_001552"
     CDS             complement(16237..16800)
                     /gene="ppdB"
                     /locus_tag="ACHKJF_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0948932.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16791..17261)
                     /gene="ppdA"
                     /locus_tag="ACHKJF_001553"
     CDS             complement(16791..17261)
                     /gene="ppdA"
                     /locus_tag="ACHKJF_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0948933.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17446..18240)
                     /gene="thyA"
                     /locus_tag="ACHKJF_001554"
     CDS             complement(17446..18240)
                     /gene="thyA"
                     /locus_tag="ACHKJF_001554"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0948934.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18247..19122)
                     /gene="lgt"
                     /locus_tag="ACHKJF_001555"
     CDS             complement(18247..19122)
                     /gene="lgt"
                     /locus_tag="ACHKJF_001555"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP0948935.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19273..21519)
                     /gene="ptsP"
                     /locus_tag="ACHKJF_001556"
     CDS             complement(19273..21519)
                     /gene="ptsP"
                     /locus_tag="ACHKJF_001556"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0948936.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21532..22062)
                     /gene="rppH"
                     /locus_tag="ACHKJF_001557"
     CDS             complement(21532..22062)
                     /gene="rppH"
                     /locus_tag="ACHKJF_001557"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP0948937.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22415..22561)
                     /gene="ygdT"
                     /locus_tag="ACHKJF_001558"
     CDS             complement(22415..22561)
                     /gene="ygdT"
                     /locus_tag="ACHKJF_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP0948938.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22747..23436
                     /gene="mutH"
                     /locus_tag="ACHKJF_001559"
     CDS             22747..23436
                     /gene="mutH"
                     /locus_tag="ACHKJF_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP0948939.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23505..24218
                     /gene="ygdQ"
                     /locus_tag="ACHKJF_001560"
     CDS             23505..24218
                     /gene="ygdQ"
                     /locus_tag="ACHKJF_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0948940.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24356..24574
                     /gene="ygdR"
                     /locus_tag="ACHKJF_001561"
     CDS             24356..24574
                     /gene="ygdR"
                     /locus_tag="ACHKJF_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP0948941.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSNDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24682..25722
                     /gene="tas"
                     /locus_tag="ACHKJF_001562"
     CDS             24682..25722
                     /gene="tas"
                     /locus_tag="ACHKJF_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP0948942.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAT
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25754..26947)
                     /gene="lplT"
                     /locus_tag="ACHKJF_001563"
     CDS             complement(25754..26947)
                     /gene="lplT"
                     /locus_tag="ACHKJF_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP0948943.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(26940..29099)
                     /gene="aas"
                     /locus_tag="ACHKJF_001564"
     CDS             complement(26940..29099)
                     /gene="aas"
                     /locus_tag="ACHKJF_001564"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP0948944.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWLDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29685..30716
                     /gene="galR"
                     /locus_tag="ACHKJF_001565"
     CDS             29685..30716
                     /gene="galR"
                     /locus_tag="ACHKJF_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP0948945.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30723..31985)
                     /gene="lysA"
                     /locus_tag="ACHKJF_001566"
     CDS             complement(30723..31985)
                     /gene="lysA"
                     /locus_tag="ACHKJF_001566"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP0948946.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32108..33043
                     /gene="lysR"
                     /locus_tag="ACHKJF_001567"
     CDS             32108..33043
                     /gene="lysR"
                     /locus_tag="ACHKJF_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP0948947.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33030..33722)
                     /gene="ygeA"
                     /locus_tag="ACHKJF_001568"
     CDS             complement(33030..33722)
                     /gene="ygeA"
                     /locus_tag="ACHKJF_001568"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP0948948.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33851..35269)
                     /gene="araE"
                     /locus_tag="ACHKJF_001569"
     CDS             complement(33851..35269)
                     /gene="araE"
                     /locus_tag="ACHKJF_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP0948949.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35584..36345)
                     /gene="kduD"
                     /locus_tag="ACHKJF_001570"
     CDS             complement(35584..36345)
                     /gene="kduD"
                     /locus_tag="ACHKJF_001570"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP0948950.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGISGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36375..37211)
                     /gene="kduI"
                     /locus_tag="ACHKJF_001571"
     CDS             complement(36375..37211)
                     /gene="kduI"
                     /locus_tag="ACHKJF_001571"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP0948951.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37499..38680)
                     /gene="yqeF"
                     /locus_tag="ACHKJF_001572"
     CDS             complement(37499..38680)
                     /gene="yqeF"
                     /locus_tag="ACHKJF_001572"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP0948952.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PHTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38935..40164
                     /gene="yqeG"
                     /locus_tag="ACHKJF_001573"
     CDS             38935..40164
                     /gene="yqeG"
                     /locus_tag="ACHKJF_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0948953.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        10077 a        10934 c         9781 g         9682 t
ORIGIN      
        1 tctgtccacc cttctgttat aaacaacttc aataagctcc gtgatttttt gagacagctc
       61 aataatcctt actgattcat aatcaactga attttcataa ttatttaaaa tatgcaatgc
      121 tataaaacca atctcatcat ccggtagttg aacgctaaat tgctcattaa tgattgttaa
      181 tgcatcacga cttattgcat actctgcttt gtacaacgct ttgatttcct caaggaaaac
      241 attattaatt tgtatgcctg agacgactcg ggatattgca ttcgaaatgt gatcagcaag
      301 aatcagtagt agcgatggat tgagtttttt ttcgagatta ctctccgcat aatgcactat
      361 cttttcggca acaaaaatat actcaatacc cacatgctca atcaatttgt agagtttatt
      421 tttttgctca tttctgacat aaaaaatccg gtttgcagga tgctcaggga ccgacatacc
      481 atatttttta ttgtaaccaa cgccaggccc agaaatgata acctcctgtc cgttcaccga
      541 tgcctgtaca caattattgt tcatgacttt ttcgatgatc attccatggc tcccaaaaaa
      601 aaaggcaagg catgtcagaa gtaataatta cttcaaacat tgccttgcct gatttaacag
      661 ttacaagata attaactaat gcgtatacct tacacggaat tgtgcttaat aaaagagatg
      721 acaggcttat tttttaagcc gatattcaaa tattcgacag cagtcacaaa accatcgttc
      781 aacatttatg taaataatat ttattcaatg aaacataaat taattgaata aattaaaaac
      841 atcatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatc cccgcaagaa
      901 tagactccgc aacttcctgc tgaaaggtcg ccgttttcag tttacgctct tcctcaacgt
      961 tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt agtacggcaa
     1021 acccggcctg ttcaacctga tttttatgca gcttgttgat tttaccgagc ttattcagta
     1081 ccgctttacc aaacttaagg ctgtcagcaa tggtcagcga ctgcaccata tcgaacatgg
     1141 tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc gaggcgttct
     1201 gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag agcgcaaaca
     1261 cagaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag acaaacaggt
     1321 cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat
     1381 tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc aaacggcgag
     1441 ctatttgcaa taccacatct ttttctcgcg ttttgtattt ccccaccgca ccggagtctt
     1501 cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct gctttacccg
     1561 gttgtggacc actttgtgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct
     1621 ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag aggtccatca
     1681 ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt tttacgtttt
     1741 gctttaattc aaaaaccatc cgtacggtct gcgggtcaaa ttgcccgacg cgcgccgact
     1801 tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt
     1861 ttacatcttc gatatccacc accacgcgtt ccgggttact caacgcgaac tgcttatatt
     1921 tcagctgacg atttgattct accgtcacac gggtgtagct ggacgcaggc cagacgcgca
     1981 ccgccacgac ctggctgacc gcagccagac tgacctgact tacgctcaat agccacatgg
     2041 cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc
     2101 tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt
     2161 tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg
     2221 cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg cgaaaaaaca
     2281 gaataaaaat acactaattt cgaataatca tgcaaagggg tgtgccgtgg taaaggaacg
     2341 taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg
     2401 aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat
     2461 cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc
     2521 acgtccgcag atcgacgcaa atctggccgc gcatcaccac gaaccgctgt atcacaagaa
     2581 tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca
     2641 actggacatc actgctcgcc tgtcgatgag tctcaataac acgccgctac agggcgcgca
     2701 tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg atgacggcgt
     2761 ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact
     2821 ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt
     2881 taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag ctgaaaagat
     2941 gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg tctccgaact
     3001 tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg attacaactc
     3061 cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg
     3121 tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg
     3181 tatcggtacg cagattgtga tggaaagcgc tgagcagatt cgtcgcgcaa caatcaacga
     3241 tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc tggtgcgccg
     3301 ttctcgcgag cagctggaga tggaaatcga taaattcacc attattcagc gcgataacac
     3361 gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg
     3421 tgtggcagtt cacccggatt accgcagctc atcacggggc gaggttctgc tggaacgcat
     3481 tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca cgcgcagtat
     3541 tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc ccgagagcaa
     3601 aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt tagggtaagc
     3661 gatgaaaatt gtcggatgcg acatgcgtaa cactcgtgcg tcgcatccgg caattacgtt
     3721 tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg agtggcgatt
     3781 gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcacc
     3841 gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc
     3901 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc
     3961 tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg
     4021 cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg
     4081 ctgtcattac gggcaatcat caccggtcga ccttcgtacc aacgagagtg cggatgacga
     4141 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
     4201 aacggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg aatgattaaa
     4261 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc aagagcttcc
     4321 tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat atcagtaaaa
     4381 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc cgcagctaac
     4441 tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg
     4501 cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt caggcggctt
     4561 agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat atcgcccagc
     4621 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
     4681 tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca
     4741 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
     4801 cccggctgcg cacccagcaa tcggtgcaaa gtactggcat cttccggaat gcgtttcttt
     4861 tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg
     4921 gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga
     4981 attaacgctg ccagtaactt cgccacggtg gtcgttttac cggtgcctgg gccgccggaa
     5041 atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca gttaatttca
     5101 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
     5161 gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca catgcgattc
     5221 aaataaagac gatcgccaca aaggatcatc ggcgtgggtt catctcccct gctgaccgct
     5281 tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
     5341 gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa acagacgtgt
     5401 ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct
     5461 cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg ctccacagct
     5521 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
     5581 catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct tgttgcggat
     5641 gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga cgatcatagt
     5701 cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa agctgatatt
     5761 gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg taagccgaac
     5821 tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
     5881 ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc agcggcgggc
     5941 agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
     6001 gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg gaaagctgac
     6061 tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc cactcggtca
     6121 acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg
     6181 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
     6241 acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg ctgaccacgc
     6301 ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct
     6361 gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agcgtcctcg
     6421 cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca ccagtttgtg
     6481 ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg
     6541 catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt
     6601 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
     6661 ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg
     6721 caagctcgac gctttctggc gcagcgttaa gatccagaac agcctcaaac gagtggcgat
     6781 cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac cagaccagcg
     6841 gatattccag cccttttgat ttatggatcg tgacaatctg caccagatgt ttatcacttt
     6901 cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt tgcgataacc
     6961 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
     7021 tatgcaagat atcggtaagt cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa
     7081 tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
     7141 gccgataacc atcgaactct tcgaccaccg cgtcccacgc atgttcgtca ttattcagcg
     7201 tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg cgcagggtgt
     7261 tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag
     7321 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg
     7381 catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
     7441 gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
     7501 gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgataa tcgccaacgc
     7561 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaata
     7621 caaaacgtaa cgcctgattt ttcccgactg atttcactgg aataaacggt atttcgcgaa
     7681 acatgaacgc gtcatcagtc tggctgaaaa gcttattcac gctgttcacc attcctggtg
     7741 cggaacgcca gttggtgtct aaagtgtagt gggcgtgaac ttcgctacgc gccttcatat
     7801 aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg ccaatcagca
     7861 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
     7921 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
     7981 acacctcacc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
     8041 ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca gccaatgcgc
     8101 gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa
     8161 acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag aaacgctggg
     8221 agaatttttc cagcgactcc ggcaactgat aactgttggt ctcttcttct gcccaggcgc
     8281 tgatcttgtc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
     8341 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtag
     8401 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg
     8461 ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
     8521 gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg tagcagtggc
     8581 gacgccagaa atcggcgcag gcctggtaac gtagcagaga ctcatcttca atcagctgct
     8641 gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaaaccgt
     8701 gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg
     8761 cttgcgcttt atcgtcgatc tcttccagca gacgttcgta cagtggattg tcggtggttt
     8821 cacgcagaca ggcgatgcgc aactcatgga tattgctacg gatacgacca cgcaattctg
     8881 ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa
     8941 aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
     9001 ttttgcctgt gccggcagag gcttcaatca ggcgctcacc ctgtaagggc aagcgcaaag
     9061 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
     9121 aacgcgctga cgttctccca cactttccag ccttcagggt gtacatattc ggctttccca
     9181 ttctggctgc cggaaatctg cgacaggata gccatgcctt gcggctcgac taccgcctga
     9241 tggaagaaat cggccagttt ttgcggcgtc agcagtttaa tctgggccac gattttatca
     9301 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
     9361 ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac
     9421 tcttctggct tcatcgcccg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
     9481 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
     9541 cgccccacgc tcattgggaa tgcaaacacg gcatagccca actgttcttc ggtacgcaac
     9601 tgattgtaga accacggctg tacgatctgc cccaacagtg agctataggc tgagctggtg
     9661 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg
     9721 gctttttcaa agatgacgga ttgtttttta tcgaccacga catctttgtt acgacaccac
     9781 tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggct
     9841 tcggtcatgt tgccaataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga
     9901 taggccagca cctctttcaa cgtaatggag ggcaaaattt tgcgccgttc atctcgcgag
     9961 aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcgaa cgctttgccc
    10021 ttttctgcgg aatccatcat ctggttatac caggactttg cctgctcaag ctgatcttcc
    10081 gtggcggtat agctaaagta gccctccagc aatgcctgga acagctgcgg cagacgctgg
    10141 gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa acttatgcca
    10201 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
    10261 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
    10321 acatcagctt tgggttcgct ggaaaaataa cggcttggcg catacaccac acgcagattc
    10381 gactcatcaa caatcagctc tggatggtca tatttcttct ctgacttaat cagcgagaaa
    10441 tcgtcaggaa tataggggtt aagctctggc aaagagagcg caatgtcggc ggcttttttc
    10501 tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc atcgacaaaa
    10561 taagccgttt tattgtgcgg ctctttcggg ctgatatacc agatacgcgc attctgcggc
    10621 gtcatcatcg ccagacgttc ttttactgct ttagcatcgt accgatcggc aatattgact
    10681 gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa
    10741 tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt cgccagttca
    10801 tcgaagtatt gtttatcaat ccctttttca cgtaacaaat tgaggtagct aaaaatggcc
    10861 gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga gatcgctaat
    10921 acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc
    10981 tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag ataggtaatc
    11041 aattcatccg ttttactacg gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc
    11101 agaactttac gcggcagtgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    11161 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaggtg
    11221 tccgccgcca tttttgccaa ctccggcaac ggtttattac tgtaaataac cgccttcatc
    11281 aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac cggattacca
    11341 ggtttgtcgc ttaaagtttc gaggttaccg cctgaaaact ttgaaccagg gtgcgccggg
    11401 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    11461 aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag caagggttct
    11521 gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc aacttccaga
    11581 tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga
    11641 tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact catatgttca
    11701 aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcaccacc
    11761 agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta
    11821 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    11881 atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac taacaataat
    11941 aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact taattttctg
    12001 tcagattcaa cacgttaacg caaatcatct gccagaattt aatcttgtgc tgcacgagtc
    12061 agcctatgtt tatataacca tcagtccgtg actggtgcgc atcataaagt aagcggatag
    12121 attgcgcaat ttttatacag cactcatgac tgattaaagc gaaacagcgg taacaggaaa
    12181 cgttgcgact gttcaacgat ggcctccatt gtctctggta ttaattgccg ccagagcctt
    12241 tgataccaga tatcatcacc ttcgccacgc accatcatgt tgccttcgta agcctgaagg
    12301 aatttcgtac gggctttttg caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg
    12361 tcataacagg tttttagcca cgcgccgcca ctttcaggta acaccagcaa tggcgcggac
    12421 atcccttcac gatacccctc aatcagttgt gagaggtaat gcaaagcctg ttcggctgca
    12481 agcggcggaa aacgccactc gccgtctttg cgtagaaaaa ggcgactttc accattacca
    12541 ccgctggcac agtagacaag gtgttccagc caaagttgca ttccctgcgc cacacttaat
    12601 aaagagggac gccagcgcaa caagccatcc ggctgcacct gcggcaacca gccagttatc
    12661 tgcacaccgt tgcaggcgag atcaatttcc atactctgcc ccggctggcg acaggcaatg
    12721 actctgtcgg caagctgctg catctcctgg cactgtgttt cccagaaaat ttcaccaaaa
    12781 gcgccatacg gtaaatcccc tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc
    12841 tgctcaacca gtgcattcaa taactgctga ttgatttgat aacgactaag cccttcaaga
    12901 ataaatggct cggtgtcggg gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc
    12961 atctggaaga acgcccgcac cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg
    13021 gtttccggta aggtaaacgg cagcggctga acaaattcag aatgtgcttt accagcctgg
    13081 ctggccgcag gtagccattc acgagcatag ctttgtcgtt cgcctggctg gtagttttgt
    13141 ggatcaaacg gcatccgggt atggaggcaa gtaagatgcg cttttaccct tgcctcgctt
    13201 tcatcacagt tgagcgcttc atcgcccggt agataatgac tttgcccgat gtagtcgatc
    13261 agttcctgca ccagtaccga cgggaaacgt tcactgttat cctgaatgga gcgcccgata
    13321 tagctgatat aaagtttttg ttgcgcggaa attaacgctt ccaggaacag atagcggtca
    13381 tcatcgcgac ggctacggtc gccacgcttc ggtttctggc tcatcaggtc aaagcccaat
    13441 ggcgcaagct gacgtggata aacgccgtcg ttcattccca gcaggcaaac cactttgaac
    13501 ggaattgaac gcattggcat cagagtacaa atgttaaccg gtccggcgag aaaacgctgg
    13561 ctgatacgtt cttgatccag gcgctgtgcc agttcatcac gcaatagtga cagcggcacc
    13621 gcgtcgccat actgcgcacc taaaccttcg gcgataatcg cctgccattg ttgttcgatc
    13681 agcgtcatcg ccgcttcggt ttccgcatcc ggcaggaaga aggcgttgag catatcgcga
    13741 caaaccggca accactcttc cagcggacgc tcctgtgcca gcccgcgacg ccagatgttt
    13801 agctgcatta gcagggaagc cagatgcccc accagttctg caattaagcc gctcgattca
    13861 tcataaggta gaaccgattg ccactcgccc tgcgcgctct ccatcgcgta acccagcaac
    13921 atgcgcgtca ggccaaaccg ccaggtgtgt tgaccggtag cggggagttc cagctcgcga
    13981 acgttgtcgt catctatccc ccaacgaatg ccggattcgt tgacccactg gcgtaaataa
    14041 cgcagccctt cttcggtgat gtcaaaccgc gccgccagca ccggcacatc cagcagcgcc
    14101 agcacatcct ctgacacaaa gcgactgtca ggcagtgata acaggctgat aaacgcttcc
    14161 agtaccggat gtgactgccg cgcacgacgg tcggaaatgg cgtaaggcag gtaacgatcc
    14221 gcaggtgcgc taccaaacac tgcctgaata aacggactgt agctgtcgat atcagccacc
    14281 atcacgatga tgtcgcgcgg agtaagtgtc gggtcttcct ccagcatcgc cagcaggcga
    14341 tcgtgtaaaa cttcaacttc acgctgcggg ctatggcaaa cgtggaaggt gatactgcta
    14401 tccagtggat caagcgggcg tttgttatcg ctacgggaaa actcttcgat gttcacacca
    14461 gcaacggcgc ggttttccag ttccagaatg tcagactgaa tgttatgcag caggttatct
    14521 ggcatcacat cgacaaaggc gtccagctcc tgactgctat ccagatcgga aaggagatag
    14581 atatagtcgc gccccaactt gccccaggag gccagtagcg ggttgccgac atcctgttca
    14641 ccgtcgctgt taaagagctg tccggcattt tcgctatcgc gaaatagcgg caattcgcga
    14701 tcttcaaaac tgtgtcgacg ctgacgagtc agcagtttcg ccagataagc aggatcttta
    14761 atatcgcccc agtaataacg gcaggggttg gtaaacagga gatggatttc aatatgttta
    14821 cccagcgcct ggagcgcctg gagataaaca ggcggtaacg cggaaatacc gcatataaag
    14881 acgcgcgaag gtaaccccgg cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc
    14941 tgatagagat tggcgcggtg ccagcgcggt tgcccgagtt gatgggtata ttccaccaac
    15001 gccttccaca acggcgcttg ccaggcctgt gcttctccca gcccttcaac caaatgtcct
    15061 gtttcccact gtgccagcca gtccggacga tagaccagat actggtcaaa caggtccgcc
    15121 gcttttgaag aaagctggaa cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc
    15181 aacagggtaa agtcttcgcg atccagcaat tgcggcagca gagtcatcag tttccagctc
    15241 atgctctgtt tgttaaaggc gctctctttg gggatttccg gtaacacccg gacgaacata
    15301 tcccagataa agctcgcagg cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc
    15361 gacagggtca tttgcagcca ctgcgccata ccggtacttt gcaccagaat catctctggt
    15421 tcgaaaggat cgtccagccg ttcgcgttcg acaataaact ccatcaacgc ttccagcacg
    15481 tccagacgat tggaatggta gacccttaac atagcggctc ctgactactg acgattcggg
    15541 caatgcaggc gggtcatctc gccttctctg cccccgggtg aaactagcgt aacgctgatg
    15601 ctgacacatc ccgcctgcga tgtctgcatt cggttgacct gccagttggc aggtggcgaa
    15661 atcgcgcgca gttgcgtttg ctgccagcca tgccgccaga gctgttggta ctggtttctg
    15721 ctggcaagac tgttcattaa tgttcgctgg aaacccgata acgcagtgac aatcatcacc
    15781 atcaacacca tcgccaacat tacctccggc aggctaaagc cttgttgatt cttcagggaa
    15841 gctgacataa cgccctctct ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa
    15901 caatgtttcc atcaatgact tcgcctgttc gccacaacga aacgccttca taaccggcaa
    15961 ccaataaggc ttcattatct gccagtaaac gcaaacaaac ccgggcatcg gttccagcgt
    16021 actgcgagca ctgaactgcg ggctgcgtct gccaggagtg cattttcccc cacgccagcg
    16081 ccgactgaac gatggcctgg cggcgcaatg actgactttc catgctcacg cgagaggcaa
    16141 aactgcgatc ctgctgactc attccttgca atagcaagct acccaaaacc agcagcatca
    16201 gaaccagagc cagtgacgaa acaccttttt cgcggttcac aggttgaatc ctgtcacgct
    16261 atagctggca tccaccacgg tttgcggttc agacttactg gcagcacgca tattaaccgt
    16321 caacaccggc gagaagccgc tgacatcctg acgtacgacc tgaaaagtgt cgataatgat
    16381 ggcatccgga ttagtgactt tatcccagcc cttaccttca caggatgtcg caccgcgtag
    16441 cgtttccagc acatgctcct tcagacgaaa tccaatctgg tcagactctt ttaccggttc
    16501 gcgatcccag ataccgttac tgttcgcatc ccactgcaca atgatacagt caccctgtcc
    16561 gacaatttcc agcccttcgc cggtacagct gccatgacaa taacccgccc tctggagatg
    16621 cttcgcgacg gtaaataccc gcagccagat ttcatcttcc agcgccagct tacgggtgct
    16681 cgttaaactt tcacgctgta acgcaggcag aaagcgtgca gcccccagca acaatacgct
    16741 actgatcgcc atagcaatca acacttccag cagagaaaaa ccttgctctt ttacaggcat
    16801 ccttctgttt ctccttgctg acaaagccgg agtcttcccc acggcgaaac caccagccac
    16861 cactcgcccg ttgagttttt gaagcgaata tgcccggccc atgcggtatt gcgcaggcca
    16921 aagaaagcaa gcgaaggtgt caggtcgctc atttcgactt cgggccagcg tggcacaaag
    16981 accaatggtg aactgccatg acaggtattg gccccagcag cggaactcac aaggcaccat
    17041 aacgtcccct ccctgataac gctgatactg tggtcgcggt tatgccagtt ggcatcttca
    17101 cgtaaataga gcaaatagtc ccgcgcctgg ctggcggttt gccatagccg ttgcgactgc
    17161 tgccagtatt gccagccata gagtccactt gcgcttagca tgaccaaaat cagcatcgcg
    17221 accagcgttt caatcagcgt ataaccacgt tgtgttttca tgccggcagt atggagcgag
    17281 gagaaaaaaa gacgagggcc agtttctatt tcttcggcgc atcttccgga ctatttacgc
    17341 cgttgcagga cgttgtaaaa tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa
    17401 aaaaccgacg cacactggcg tcggctctgg caggatgttt cgtaattaga tagccaccgg
    17461 cgctttaatg cccggatgtg gatcgtagcc ttcaatctca aagtcttcga aacggtagtc
    17521 gaagatggat tcgggtttac gtttgataat caacttcggc agcggacgcg gttcgcggct
    17581 taattgcaga tgagtttgat ccatatgatt gctgtacagg tgcgtgtcgc cccccgtcca
    17641 gacaaaatcg cccacttcca gatcacactg ctgcgccatc atatgcacca gtaacgcgta
    17701 gctggcaatg ttaaacggca ggccgaggaa aacgtcacag gagcgctgat aaagctggca
    17761 agagagtttg ccgtctgcca catagaactg gaagaacgca tggcacggtg ccagcgccat
    17821 tttatccagt tcgcctacgt tccacgctga aacaataatg cggcgcgaat ccgggtcgtt
    17881 tttcagctgg ttcagtaccg tagtgatctg gtcaatatga cgaccatctg gcgttggcca
    17941 ggcgcgccac tgtttaccat acactggtcc gaggtcgccg ttttcatcgg cccattcgtc
    18001 ccagatggtg acattgtttt cgtgtagata agcaatgtta gtgtcgccct gcagaaacca
    18061 cagcagttcg tggatgatgg aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc
    18121 atcctgcagg ttaaaacgca tctgatgacc aaaaatggaa agcgttccgg ttccggtacg
    18181 gtcgtttttc tgtgtgcctt cgtcgagcac tttttgcatc agttctaaat actgtttcat
    18241 ggttcctcag gaaacgtgtt gctgtgggct gcgacgatat gcccagacca tcatgatcac
    18301 acccgcgaca atcatcggga tggaaagaat ttgccccatg ctgatgtact gcacccaggc
    18361 accggtaaac tgcgcgtcgg gctggcggaa aaactcaaca atgatgcgaa acgcgccgta
    18421 gccaatcagg aacaagccag agacagctcc cattgggcgc ggtttacgaa tatacaggtt
    18481 gaggataata aacagcacca caccttccag cagcagctcg taaagctgtg atgggtggcg
    18541 cggcagcaca ccgtaagtgt cgaaaatgga ttgccactgc gggttggttt gcagcagcaa
    18601 aatatcttct gtacgggagc ctgggaacag catggcaaac gggaagttcg ggtcaacgcg
    18661 gccccacaat tcaccgttaa taaagttgcc cagacgcccg gcaccaagac caaacggaat
    18721 gagtggtgcg ataaaatcag agacctggaa gaaggaacgt ttagtacggc gggcgaagat
    18781 aatcatcacc acgataacgc caatcaggcc gccgtggaaa gacatgccgc cgtcccagac
    18841 acggaacaga tacagcggat cggccataaa ctgcgggaaa ttgtagaaca gaacataacc
    18901 aatacgtcca ccgaggaaga cgccgaggaa gcccgcatag agtaagtttt caacttcatt
    18961 tttggtccag ccactgcccg gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat
    19021 gaaacccacc agatacatca ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat
    19081 gaccggatca aactccggaa aatgcagata gctactggtc atctgtcacc acaagttctt
    19141 gttatttcgc tgaaagagaa cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa
    19201 aggtgcgaat aatagcacaa ggggacctgg ctggttgccg gataccgtta aaagatatgt
    19261 atatgatccg cgctataacc ctccgcgaat cagcccgccc atgccgcgac gctccataaa
    19321 ggctgcaacc tgatggcgaa cttcggtcgc cagttgcgct tccagactac gctgcgcaag
    19381 attttctgcg tcggcataat caatgcgccg cagcaggtat tttacccgcg ctacagaacg
    19441 tccgttcata gacagatggc gatacccaag cccaatgagg attgccacgc acatgggatc
    19501 gcccgccatt tcaccgcaca aacggagatc gattccatgt atttccgctt cccgggcgat
    19561 catcgccaga gctcgtaaca ttgcaggatg aagactgtca taaatgttcg ccacccgggt
    19621 attgttgcga tcaacggcca gaatgtattg agtcagatcg ttggtgccaa cagagatgaa
    19681 atcgacccgc tttgccagat gcggcagcat aaataccatt gacggcactt ccagcatgat
    19741 gccgatacgt ggtttgggaa tttcgtaacc gatcatctcc tcgacttcac gtccggcacg
    19801 ttcaatcagg cggcgtgctt catcaacttc atcgaggctt gtgaccatcg gcaacagaat
    19861 attcaggttg cccgtagcgg cattagcacg cagcatcgcc cgcacctgga tcaagaagat
    19921 ctccggctga tcgagcgtaa tgcgaatccc acgccaaccc aggcatggat tctcttcgct
    19981 gatcggcatg taaggcagct gcttatctgc tccgacatcc agcgtacgca aggtgacggg
    20041 tttatcattg aacatttgca gcatcccctg atactgcgcc acctgttctt cttccgacgg
    20101 gaaaccactt tgcagcatga atgggatttc agtgcgataa agaccgatgc catcaatacg
    20161 gctgcccagt ttttcttcat gttccgggct taaaccagca ttgagcatga cttttatacg
    20221 ctcaccgctt tttaactgcg cgggtaaatt gacgtcatct tccgccagac ggctaagctc
    20281 aatctcttca ctaattagcc gctgatattc ttgcagcagt accggctccg gatcgaccag
    20341 caattcaccg cgatagccat caacgatcag cgtcctacga tgcagcaccg aaggctgaat
    20401 atccgcgccc atcacggtag ggatccccag cgcacgtacc atgatcgcag catgggagtt
    20461 ggctgcgcca tctcgcacga caacaccgac taagcgatcc tggggcagct cagcaagagt
    20521 tgtcgctgac agttcatctg ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg
    20581 attagcgtca tcaagatgaa acagcaatcg ctgacccagc gcacgtaaat cgccagcccg
    20641 ctctttgaga tagttatcgc ttagcgcggc aaactgttcg gcaaattttt caatgaccgt
    20701 ttttaccgcc cactctgcca ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag
    20761 ccgggtgtcc gaaagcaggt gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc
    20821 tccggcggca aagcgtttgc tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc
    20881 ggtcaatcgt tcgcgttcca gagccggatc cagcgttgat gcctgataca cctgttccat
    20941 taaaggtaac gtggcatcct gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa
    21001 tgcgcggatt cgcgtctggc gatattgccc aaacaaggca gtcaactgcg actgagaaag
    21061 aatagctgcc atctgggtgg caagcgtcac caggaaggat tcttcacttt cgtcatactg
    21121 gcgcaactct cgttgctgta ccaccagtac accaagcaac tggcgacgtt gaataattgg
    21181 tacgcctaaa aacgcgcgga aacgttcttc ttttacggag gggatgtatt tgaagctggg
    21241 gtgcttttgc gcatctgcaa ggtttatcgg ttccgccagc ctgccaacca ggccgacgat
    21301 cccttcatca aacgcgagcg ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc
    21361 catcaggtag taacaacgtc gatcatgatc ggccaggtag accgaacaga cctcggtatc
    21421 catcgcaaga cagatgtcgg taaccagaat atttaacgcc tcattcaggc gtggtgcgct
    21481 ggctaccttt tcgactattt cgcgcaggcg agtgagcata attggcgtga cttaacctct
    21541 tttacgtcga taagcagatg cgttttgtgg ttttggcgta ttttcctgca gtgacatcac
    21601 cacactcgcg aactctttca ttaccctacg gtagacatca cgtttaaatg acaccacctg
    21661 tctgaccgga taccagtaac ttacccatcg ccagccgtca aactctggtg tactgctggt
    21721 ttgcatattg atttctgcat cgccgctcac cagctgcaag agaaaccatt tttgtttttg
    21781 gccgatacaa accggcttcg tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa
    21841 ccagttacgc gttgaagcaa ggattcgaac gtctttgcgg cttaatccta cttcttcaaa
    21901 caattcacgg tacatcgcct gctctgcgga ttctccgggg ttgattccgc cttgcggaaa
    21961 ttgccaggag tgctgaccaa atcgccgggc ccacattacc tgcccctggc gattacaaat
    22021 cacgatacct acgtttgggc ggtagccatc gtcatcaatc accggactac ctcaaaataa
    22081 agctttatat acgaatgatt gtttcatact ccaggaagac ggtaaaccac tctctgcagg
    22141 gcattacaca ctaataacaa ttgaataact cacagttatg tgcagagtta taaacagagg
    22201 aaggggtgga tagccgtttt tattcacctt ttctgtggat agagttgtga agaactacgg
    22261 aattactacg ggaaaacccg gagcattctg aataagcccg atatagacat tttaaaatat
    22321 tcattattag tcaatgcatt acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc
    22381 aggtcacacc tctcatttac gggttggcga aagatcaacc aatgccgtat ttatccacag
    22441 aatgtgccac taagttaagc actgaaccac taaaaactgg agtttcgtcg cacgtcaagg
    22501 ctgtaaatgg aaacagtagt ggaggttttt cacagttatc ccagctttct gtggataaca
    22561 tggtgtaaga tcctgtttat tttcagtgac cagatttgga aaacccgttg cagtgttgcg
    22621 caactcgatt accggcagcc taaaaagcaa tataaatcag ggaattgaac aacacatgtg
    22681 gaaaaagtta cactgcgaat attcggcaca taattgctgt ttgtttttta atcaaggtat
    22741 catgacatgt cccaacctcg cccactgctc tctcctcccg aaactgaaga acagttgtta
    22801 gcgcaagcac agcaactttc tggttataca ttgggagaac tggcggcact tgccgggctt
    22861 gttacgccag agaatttaaa acgcgataag ggctggattg gcgtgttact ggagatctgg
    22921 ctaggtgcca gcgcagggag taaacctgag caagattttg ccgctctggg cgtggaactt
    22981 aaaactatcc ctgtggatag tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc
    23041 ccgttaacgg gtaatagcgg ggtgacctgg gaaaccagcc atgtgcgcca caagctcaaa
    23101 cgcgtactgt ggataccggt tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta
    23161 ggatcaccgt tgctgtggag cccgaatgaa gaggaagacc gacagctacg cgaagactgg
    23221 gaagaattaa tggatatgat tgttctcggt caggttgagc ggatcaccgc tcgtcacggg
    23281 gaatatttac agatacgacc gaaagcagcg aatgcgaaag cgcttaccga agccattggt
    23341 gcccggggcg aacggattct gacgctgcca cgcggctttt atttgaagaa gaatttcacc
    23401 agtgcactac tggcccgtca ttttctgatc cagtagccat cgctttgacc tgccgctttc
    23461 cgggcatata attaccgctt catttttttg gcagggcttt ttagatgtta tttgcatgga
    23521 taaccgatcc taacgcctgg cttgcgctcg gtacgctgac gctgctggag atcgttcttg
    23581 ggatcgacaa tattattttc ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg
    23641 ctcatgcgcg ccgtctgggg ttggcgggag ccatggttat gcgtctggcg ctgctggcat
    23701 caatcgcctg ggttacgcgc ctgacaaatc cgctttttac aatattcagt caggaaattt
    23761 ctgcccgtga tttgattctg cttctgggtg gcttgttcct tatctggaaa gccagcaagg
    23821 aaatccacga atccattgaa ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc
    23881 tcggcgctat cgtgcagatt atgctgctgg atattatctt tagcctcgac tcggtgatta
    23941 cggctgtggg tctgtcagat cacctgttta ttatgatggc agccgtggtg attgccgtag
    24001 gcgtgatgat gttcgccgcg cgctcgattg gtgattttgt cgaacgccat ccttcggtaa
    24061 aaatgctggc gctctctttc ctgattctgg tgggctttac cctgattctg gaaagtttcg
    24121 acatccacgt accgaaaggt tacatctact tcgcgatgtt cttctctatt gcggttgaaa
    24181 gcctcaactt gattcgcaac aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg
    24241 gagggcgtac tcactccttt taccctcaca gtttaagatt ttcctgcttt caagatatat
    24301 aacgtcggtt tataaacaga ctattatcat aggtaggcca aatgaggata accggatgaa
    24361 aaaatgggca gtaataattt ctgcagtcgg actggcgttt gctgtttccg ggtgttccaa
    24421 tgattacgtc atggcgacca aagatggccg tatgattttg accgatggaa aacctgaaat
    24481 tgatgatgat accgggctgg tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa
    24541 ccgtgatgat gtttcgcaaa ttattgaacg ttaacaaata aggtcagcat ccggctggcc
    24601 ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc cttatgtcgt
    24661 gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc gctggaagtc
    24721 agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc cgacgcccac
    24781 gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc cgaaatgtac
    24841 ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg
    24901 gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc
    24961 aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat ccgcgaagcg
    25021 ctgcatgaca gcctcaagcg cctacagact gattacctcg atctttatca ggtgcactgg
    25081 ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga ttctgcgcct
    25141 gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc gggaaaaatt
    25201 cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct gcatctggcg
    25261 gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct gttaaaccgc
    25321 agttttgaag taggtctggc agaagtcagc cagtatgaag gggtcgaact gctggcctat
    25381 tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa acccgctggc
    25441 gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc
    25501 gtcgcgacgt atgttgatat cgccagacgt catggcctgg accctgctca gatggcgctc
    25561 gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac tacgatggat
    25621 cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt attagctgaa
    25681 attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg ccggttaaaa
    25741 ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt gccagcgcaa
    25801 acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctatcatt accgccagcg
    25861 agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt actgcaatcg
    25921 cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg agcggaacga
    25981 caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc agcagctcgt
    26041 gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc atacagcgtg
    26101 acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg ataccaatcg
    26161 ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc gccaccggta
    26221 cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa ctggtgccca
    26281 ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc aggaaactgc
    26341 gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt ttgggaatgt
    26401 agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg gcgacgagga
    26461 catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc gctatggtag
    26521 aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat tcgccgagaa
    26581 taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta tagccgagaa
    26641 acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg ttggcaaaca
    26701 tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc gcaaaaagaa
    26761 tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga tagaactgcg
    26821 ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca gagagaaact
    26881 gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg tgcactgact
    26941 cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg
    27001 ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc
    27061 ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc gttatctgtg
    27121 gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt ggcatggact
    27181 ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac catttcgcct
    27241 gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg ctcgtcaaaa
    27301 cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac attctcggcg
    27361 gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat tatgttcggc
    27421 cctttcagtt gcaggcgtcc gccctcttcg ataccaggaa ccgacaacag gcgcgcatcc
    27481 attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac gttgatagaa
    27541 acgacaggcg cgcactcggt tacaccatag ccttcaagga tgcgcaggcc aaatttatcc
    27601 agccaaagct gtttggtact ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga
    27661 cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt cgaggtaccg
    27721 aacaacacgg tgcaactgcg gtcatacacc aactccggca caatgcggta atgcagcggg
    27781 cttggataaa ggaacacttc tgcacctgta agcagtggcg taaacaggcc taccgtcagc
    27841 ccaaaggagt gaaacagcgg taatgccgac ataaagcgat cgttggtggt gaagtcggca
    27901 atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac gacgcctttt
    27961 ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg ctgctgtttt
    28021 acctgcgcca ggcgcggcat cagcaaatga gcgaagatcc atactttgtc ggcagtggtg
    28081 acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg ctccggcaga
    28141 tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt gatttcagcc
    28201 gccgtaatag cactggtcag cccttttacc ccagcagtgt agttcatcat tgcgggaatg
    28261 cggcgacgcg cgatggcccc aaaaatcact gccgcactga tgcctgcatt gggcagcatt
    28321 aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac aaacagcgtt
    28381 ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac acatttcttc
    28441 ccggctccga agcggtacat tgcactcagt aaagattcgt acagagtttc acgcgggcgc
    28501 accgccatac gcgcttccat cattatttga tgcagcattt cgccagcgat tttgcgacgg
    28561 tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat atgcagagta
    28621 atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg cgtaagttcc
    28681 gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac aaaacccgcg
    28741 ccatcgtaga ttttcatcag cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc
    28801 actggtcggc cctgttccac cagacgtacc agatgtttaa tagccatagg ttgcgtcggg
    28861 tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg ttggcttatt
    28921 gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca
    28981 ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca
    29041 cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt
    29101 ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac gagataaaga
    29161 acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca
    29221 agagacaggg tacgaagagc gtaccgcata atctcaccaa tcaatacctc tgggatcttg
    29281 attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg aagggaaatg
    29341 caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcca aaaaaaacct
    29401 gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact tcacctatca
    29461 atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagcg gtcaatcgca
    29521 acagcgtttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct tacttatttg
    29581 cgtttggctc acattcccac gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc
    29641 gtaacactga aagaatgtaa gcgtttaccc actaaggtat tttcatggcg accataaagg
    29701 atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac
    29761 ctaaagccag cgaagcttcc cggctggctg tgcatagtgc aatggagtct cttagctatc
    29821 acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg
    29881 ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa caggtggctt
    29941 atcacaccgg taatttctta ttgattggca acggttacca caacgaacaa aaagagcgtc
    30001 aggctattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga
    30061 tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg ctgatcaacc
    30121 gtatcctgcc tggctttgaa aaccgttgta ttgctcttga cgatcgttac ggtgcctggc
    30181 tggcaacgcg tcatttaatt cagcaaggtc atacccgcat tggttatctg tgctctaacc
    30241 actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt gctgaaagtg
    30301 gtattgcggc caatgaccgg ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac
    30361 aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc tgttataacg
    30421 attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat gtaccgggtg
    30481 agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgc ccgcgcctga
    30541 ccaccgtgcg ttacccaatc gtgacgatgg cgacccaggc tgccgaactg gctttggcgc
    30601 tggcggataa tcgccctctc ccggaaatta ctaatgtctt tagtccgacg ctggtacgtc
    30661 gccattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc gactaaccgc
    30721 agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg aatcaaccgc
    30781 gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta gttggatgac
    30841 attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc cttcacttcc
    30901 ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc gcccgattca
    30961 cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga acgaccatca
    31021 gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat cagatcgttg
    31081 aacccagcat caaccagcac aaagtggcgg ctacccattt gtttgacgct ccgcacctga
    31141 gtaattaata cgccagactg cgctaccagg aagcgacccg gttcaatttc cagtttcaca
    31201 gggtggccca aatggcgggc gatttgctca cgcgcggcat tccacagacc ataataatgt
    31261 tcggtatcaa ccgcctcttc accctgttga taaggaacag aaagcccacc gcccgcagaa
    31321 atagcctgta aatcctgacc gaattcgatg acctgacgca ccatagcacc acacacctgt
    31381 tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac cagctgcaga
    31441 tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg
    31501 ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc cggattaacg
    31561 cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa
    31621 cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg atcgataaca
    31681 tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc cgccaacgca
    31741 cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg cattaagcgc
    31801 aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc
    31861 gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca gccaaattca
    31921 gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt
    31981 ggcatagcaa actccagata agtgcttttt ttatgattac gccacatcat aaaaagaata
    32041 aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag
    32101 agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc
    32161 cggaagtctg actgaggcgg cacacctgct acacacctca cagccaaccg tcagccgcga
    32221 actggcgcgc tttgagaagg tgatcgggct gaaattgttt gagcgcgtac gtggacgatt
    32281 acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact
    32341 ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat
    32401 tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc
    32461 acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg
    32521 gctctcggcc cagcgtcatg atttaggact cactgagacg ctccatacgc ctgcgggaac
    32581 agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct
    32641 ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca tcagcctttc
    32701 ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg
    32761 acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt
    32821 aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt
    32881 gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc
    32941 gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcggggttac cgaaactggt
    33001 cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg
    33061 gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg caccagtaaa
    33121 ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag gcgagcaatc
    33181 acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa
    33241 ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt gatggaaaat
    33301 tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc
    33361 gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag
    33421 aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat
    33481 aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc
    33541 ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta
    33601 tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc
    33661 agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc
    33721 attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc
    33781 tcaacggcca ggtgcccaat ctctattaac gaaaaaaggg ccggatgtac agcacatccg
    33841 gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga
    33901 tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg
    33961 caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgctg tcgagcagcg
    34021 tcaggaaggt cgcgccgata atcatattcg acacccagtt cgtggtggtc gaacaggtga
    34081 tgccaaaatc gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg
    34141 cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca
    34201 agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc
    34261 ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg
    34321 taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca
    34381 tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca tagtacatga
    34441 tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac
    34501 gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt
    34561 cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca
    34621 cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga
    34681 agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac attgcgcgcc
    34741 agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga
    34801 ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa
    34861 gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acacgagcgg
    34921 cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga
    34981 tggcccccgc catcaggctg tatttacgac ccaggcggaa cgacagccaa ccattaaaca
    35041 gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc aaacgactgg
    35101 tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac
    35161 caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat
    35221 cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc
    35281 agcagagagt aagacatagt gaaaaaatac gtgaacaact cacgcaggtg tcaggtcgga
    35341 aacagcataa atatggatta aattgctgcg acatgtcgtt atgtgatgga tattccaatt
    35401 ttcaaattaa gttgaattat tgagattatt attaaccacc taattttaca gcagataaaa
    35461 ttcataaagt tcattaattg ataattaata tggattattt cataaccatg atatggatta
    35521 tgatgatcta caggtataaa aaaccctgcc atgcggcagg gtcataaaag taagaagaat
    35581 gaattaacgc gccagccaac cgccatccac ggcaatggta taaccattca cataatctga
    35641 agcgctggag gcaaggaaca ctatcggccc catcaggtca ctcggcagtc cccaacgacc
    35701 agctggaatg cggtcgagaa tttccgcgct acgttgttca tctgcccgta gttgttgagt
    35761 attgttggtc gccatgtaac ccggagctat cgcattaaca ttaatgttgt gtttagccca
    35821 ttcgttcgcc atcaatcgcg tcacacccat cacgccgctt tttgatgcgg tataagaagg
    35881 cacacggatc ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt
    35941 gccttgcgcg ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt
    36001 caggttcatg acatcgtccc agtccttttc gctgaactcg agagcatctt cgcggcgaat
    36061 caatccggcg ttattcacca ggatatcaat atgaccaaac tccgctaccg cgcgatccag
    36121 cagtccagaa atgccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc
    36181 cagcgccgtg acctgctcga tggtttcagt cggttcaacg atgttaatgc caacaatgtc
    36241 acagcccgct tgcgccagcc ccaacgccat cccctggccc agcccagtat cacaaccagt
    36301 gacgaccgca actttacctt cgagagaaaa tgcacttaaa atcataacaa taccttattc
    36361 gtttatgccc acaactagcg caaatcttta acggccacat ggtccatatc atcaaagacc
    36421 tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatga
    36481 atcgaccagc tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct
    36541 tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa
    36601 acttccatcc ggcgctcgtg agtgtgacac ggcatggtgt tccacaagtt acctggagcc
    36661 agctccgtca gccccatact caattggcag gtttccagca catccgggac aaaatatttg
    36721 ttaattgtgc gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcgtcc
    36781 ggtgtgactt ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttt
    36841 gccggagtgg cggtatcaat actggcaaag acaacttctt ttgcaccttt acccacatac
    36901 agggcgtcgc ggtgaccgat ttcatagcat tggccatcga cagtaatcgt accggcaccg
    36961 ccaatattga taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta
    37021 ccaacttccc cgccaacgga aaccgtttta gttatcggca taatgccgcc aacaataatt
    37081 cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctc ttcaaccaaa
    37141 aattcattgc gcagcccctg ggtatccagc gtttttgcgt gcgcactgtg gatgctctgt
    37201 cttacgtcca catcaacctc cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa
    37261 acactgtttc gttttctcta taaggggatg atatgcccat gggatcgggt tttcaattaa
    37321 aaaataaaac aatgtttcat ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg
    37381 aatctgtcat gagaatggaa aaatcaggga gcccggaaaa tgtatgtcga cttgccagcc
    37441 atcattattc aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct
    37501 attcgtcacg ttcaatggtc aatgccaccc cctggccccc gccgatacaa agcgttgcca
    37561 gtcctttgcg ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac
    37621 cggaagcgcc tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct
    37681 catcccactc aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct
    37741 cgataagatc aacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat
    37801 acaccggcgc aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc
    37861 gggccagcac gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag
    37921 ctgcgccatc gtttatggat gatgcattac ccgctgtcac agaaccgaga ctatcaaatg
    37981 aaggatttaa acgggctaaa ccttctgcac tggcgtcagt gtgtggctgt tcatcggtat
    38041 caacaaccaa cgtctgaccg ttactttggg tcattaccgg gacgatctca tctttaaatc
    38101 gtccggcgtc aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca
    38161 attgacggct gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat
    38221 cattgaaggc atcccataac ccatcatgca caagactgtc aaccagctgg ctattgccaa
    38281 gctgtgcacc ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc
    38341 caccggcgat gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg
    38401 ctttaagccc ggaaccgcag acgtcattaa tcgtgattgc agaaacgcta ttaggcagac
    38461 cacctttaat agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac
    38521 caagaattac ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca
    38581 cgaccagact accaagttcc acggcggaat gacccgctaa cgcaccgcga aagcagccga
    38641 taggtgtccg taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa
    38701 cagtgcaata gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt
    38761 ttatcacaaa ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg
    38821 caaccactaa cccaccaata gaggggtaga tagggcgtta atctcccata cttaacctgg
    38881 tttatggtaa attgccctcc attttgttta atttgtagat gatacgttca gataatgtct
    38941 aatatttggt ctaaagaaga aactctgtgg agtttcgcgc tctacggcac agccgttggt
    39001 gcaggcacgc tcttccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt
    39061 actgctctgg tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc
    39121 ctctcatcga aaacatcagc aggtgaaggg ataacgggcg cagtaacaca ctactatggc
    39181 aagaagattg gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg
    39241 atatatgcag tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt
    39301 gatcttcgca tccgtatgtt ggtgagtctg ggtgttgtat taatcctgaa tctcattttt
    39361 ctgatgggac gccatgcaac cattcgggta atgggttttt tggtattccc attgattgcc
    39421 tatttcttat ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacgacc
    39481 caggtagagt tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt
    39541 ttcgccttta gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat
    39601 ggcgaacacg ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc
    39661 ataagtgtac tgttctttgt ctttagctgc ctgctttcta ttccaccttc gtatattgaa
    39721 gcggctaaag aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca
    39781 gcatggctgt caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg
    39841 ggtacgtact ttggcgttat tgaaggtgcc acggaggtcg tcaaaacaac actacagcag
    39901 gttggtgtaa agaaaagtcg tgcatttaac cgcgcactat caattatgtt ggtatcgctg
    39961 atcaccttca ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc
    40021 ggcccgctca ttgccatgat acttttcatc atgcctacgt tgtcaacgta tctgatcccg
    40081 gcgcttaaac cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc
    40141 gtatcggtaa tgttctttag ctgatatcca ttgtgaatgg ttcaaaattg ctacaatttt
    40201 gaaccattcc agttttcata atgttcattc gttattattc cgcactatca cttccctcat
    40261 catatcctag tattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga
    40321 ctatttaaga tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg
    40381 cgatatagat attctcatac catgagatag ttatctgaac caccctcaac caggtaagat
    40441 taaatcagtg aaacataatc atatttaata acga
//
LOCUS       DAZGXZ010000027        40119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000027 DAZGXZ010000000
VERSION     DAZGXZ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.027"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(228..440)
                     /gene="rpmE"
                     /locus_tag="ACHKJF_001574"
     CDS             complement(228..440)
                     /gene="rpmE"
                     /locus_tag="ACHKJF_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP0948954.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            643..2841
                     /gene="priA"
                     /locus_tag="ACHKJF_001575"
     CDS             643..2841
                     /gene="priA"
                     /locus_tag="ACHKJF_001575"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP0948955.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHLL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            2997..4022
                     /gene="cytR"
                     /locus_tag="ACHKJF_001576"
     CDS             2997..4022
                     /gene="cytR"
                     /locus_tag="ACHKJF_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP0948956.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4114..5073
                     /gene="ftsN"
                     /locus_tag="ACHKJF_001577"
     CDS             4114..5073
                     /gene="ftsN"
                     /locus_tag="ACHKJF_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP0948957.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5166..5696
                     /gene="hslV"
                     /locus_tag="ACHKJF_001578"
     CDS             5166..5696
                     /gene="hslV"
                     /locus_tag="ACHKJF_001578"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP0948958.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5706..7037
                     /gene="hslU"
                     /locus_tag="ACHKJF_001579"
     CDS             5706..7037
                     /gene="hslU"
                     /locus_tag="ACHKJF_001579"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP0948959.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7104..8030
                     /gene="menA"
                     /locus_tag="ACHKJF_001580"
     CDS             7104..8030
                     /gene="menA"
                     /locus_tag="ACHKJF_001580"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP0948960.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8123..8608
                     /gene="rraA"
                     /locus_tag="ACHKJF_001581"
     CDS             8123..8608
                     /gene="rraA"
                     /locus_tag="ACHKJF_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP0948961.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8693..8938)
                     /gene="zapB"
                     /locus_tag="ACHKJF_001582"
     CDS             complement(8693..8938)
                     /gene="zapB"
                     /locus_tag="ACHKJF_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP0948962.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9363..10208
                     /gene="glpF"
                     /locus_tag="ACHKJF_001583"
     CDS             9363..10208
                     /gene="glpF"
                     /locus_tag="ACHKJF_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP0948963.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10231..11635
                     /gene="glpK"
                     /locus_tag="ACHKJF_001584"
                     /pseudo
     CDS             10231..11635
                     /gene="glpK"
                     /locus_tag="ACHKJF_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
     gene            11771..12781
                     /gene="glpX"
                     /locus_tag="ACHKJF_001585"
     CDS             11771..12781
                     /gene="glpX"
                     /locus_tag="ACHKJF_001585"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0948964.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12878..13624
                     /gene="fpr"
                     /locus_tag="ACHKJF_001586"
     CDS             12878..13624
                     /gene="fpr"
                     /locus_tag="ACHKJF_001586"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP0948965.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13629..14057)
                     /gene="uspD"
                     /locus_tag="ACHKJF_001587"
     CDS             complement(13629..14057)
                     /gene="uspD"
                     /locus_tag="ACHKJF_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP0948966.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14084..14383)
                     /gene="yiiS"
                     /locus_tag="ACHKJF_001588"
     CDS             complement(14084..14383)
                     /gene="yiiS"
                     /locus_tag="ACHKJF_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP0948967.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14595..15035)
                     /gene="yiiR"
                     /locus_tag="ACHKJF_001589"
     CDS             complement(14595..15035)
                     /gene="yiiR"
                     /locus_tag="ACHKJF_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0948968.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15136..15735
                     /gene="yiiQ"
                     /locus_tag="ACHKJF_001590"
     CDS             15136..15735
                     /gene="yiiQ"
                     /locus_tag="ACHKJF_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP0948969.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15843..16610
                     /gene="tpiA"
                     /locus_tag="ACHKJF_001591"
     CDS             15843..16610
                     /gene="tpiA"
                     /locus_tag="ACHKJF_001591"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP0948970.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16665..17420)
                     /gene="cdh"
                     /locus_tag="ACHKJF_001592"
     CDS             complement(16665..17420)
                     /gene="cdh"
                     /locus_tag="ACHKJF_001592"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP0948971.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17527..18516)
                     /gene="sbp"
                     /locus_tag="ACHKJF_001593"
     CDS             complement(17527..18516)
                     /gene="sbp"
                     /locus_tag="ACHKJF_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP0948972.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(18836..19798)
                     /gene="pfkA"
                     /locus_tag="ACHKJF_001594"
     CDS             complement(18836..19798)
                     /gene="pfkA"
                     /locus_tag="ACHKJF_001594"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP0948973.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(19979..20881)
                     /gene="fieF"
                     /locus_tag="ACHKJF_001595"
     CDS             complement(19979..20881)
                     /gene="fieF"
                     /locus_tag="ACHKJF_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP0948974.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21030..21530)
                     /gene="cpxP"
                     /locus_tag="ACHKJF_001596"
     CDS             complement(21030..21530)
                     /gene="cpxP"
                     /locus_tag="ACHKJF_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP0948975.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21680..22378
                     /gene="cpxR"
                     /locus_tag="ACHKJF_001597"
     CDS             21680..22378
                     /gene="cpxR"
                     /locus_tag="ACHKJF_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP0948976.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22375..23748
                     /gene="cpxA"
                     /locus_tag="ACHKJF_001598"
     CDS             22375..23748
                     /gene="cpxA"
                     /locus_tag="ACHKJF_001598"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP0948977.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23854..24528)
                     /gene="yiiM"
                     /locus_tag="ACHKJF_001599"
     CDS             complement(23854..24528)
                     /gene="yiiM"
                     /locus_tag="ACHKJF_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP0948978.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24677..25660)
                     /gene="kdgT"
                     /locus_tag="ACHKJF_001600"
     CDS             complement(24677..25660)
                     /gene="kdgT"
                     /locus_tag="ACHKJF_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP0948979.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(25920..26540)
                     /gene="sodA"
                     /locus_tag="ACHKJF_001601"
     CDS             complement(25920..26540)
                     /gene="sodA"
                     /locus_tag="ACHKJF_001601"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP0948980.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEASARFAAKK"
     gene            26825..27859
                     /gene="rhaT"
                     /locus_tag="ACHKJF_001602"
     CDS             26825..27859
                     /gene="rhaT"
                     /locus_tag="ACHKJF_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP0948981.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRHPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27856..28704)
                     /gene="rhaR"
                     /locus_tag="ACHKJF_001603"
     CDS             complement(27856..28704)
                     /gene="rhaR"
                     /locus_tag="ACHKJF_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP0948982.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28778..29614)
                     /gene="rhaS"
                     /locus_tag="ACHKJF_001604"
     CDS             complement(28778..29614)
                     /gene="rhaS"
                     /locus_tag="ACHKJF_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP0948983.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            29902..31371
                     /gene="rhaB"
                     /locus_tag="ACHKJF_001605"
     CDS             29902..31371
                     /gene="rhaB"
                     /locus_tag="ACHKJF_001605"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP0948984.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLKERKINDLPALIVATQSLPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVARIHSTRKTKELCA"
     gene            31368..32627
                     /gene="rhaA"
                     /locus_tag="ACHKJF_001606"
     CDS             31368..32627
                     /gene="rhaA"
                     /locus_tag="ACHKJF_001606"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP0948985.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLELD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFNRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            32869..33693
                     /gene="rhaD"
                     /locus_tag="ACHKJF_001607"
     CDS             32869..33693
                     /gene="rhaD"
                     /locus_tag="ACHKJF_001607"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP0948986.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33703..34017
                     /gene="rhaM"
                     /locus_tag="ACHKJF_001608"
     CDS             33703..34017
                     /gene="rhaM"
                     /locus_tag="ACHKJF_001608"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP0948987.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34318..34764
                     /gene="frvA"
                     /locus_tag="ACHKJF_001609"
     CDS             34318..34764
                     /gene="frvA"
                     /locus_tag="ACHKJF_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP0948988.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            34775..36226
                     /gene="frvB"
                     /locus_tag="ACHKJF_001610"
     CDS             34775..36226
                     /gene="frvB"
                     /locus_tag="ACHKJF_001610"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP0948989.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36216..37286
                     /gene="frvX"
                     /locus_tag="ACHKJF_001611"
     CDS             36216..37286
                     /gene="frvX"
                     /locus_tag="ACHKJF_001611"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0948990.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRNILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSILNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEVEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLREAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            37286..39034
                     /gene="frvR"
                     /locus_tag="ACHKJF_001612"
     CDS             37286..39034
                     /gene="frvR"
                     /locus_tag="ACHKJF_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP0948991.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(39084..>40119)
                     /gene="yiiG"
                     /locus_tag="ACHKJF_001613"
     CDS             complement(39084..>40119)
                     /gene="yiiG"
                     /locus_tag="ACHKJF_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP0948992.1"
                     /translation="RNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEA
                     PVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQG
                     PTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTIN
                     EMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELKN
                     IEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGG
                     MNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYN
                     EMVDDYNSLR"
BASE COUNT         9761 a        10398 c        10364 g         9596 t
ORIGIN      
        1 gaaccctgga caatgctacc gccatactgc gtcatgtcgc cctgacgcgc cgccggtttt
       61 ccgctcatcg taaaatcctt ttgaaataaa taagaaccag aagcctagcg ccagtcaaca
      121 acaggaaatg ataatgagtt aacaccccgg aatttttcag aatttataaa aggcaaatta
      181 caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa tccggtctta tttgctgccc
      241 gggatgttga aacgcttgtt gaagcggtca acacggccac cggtagcaac atcacgctgt
      301 ttgccagtga agaacgggtg gcacttgctg cacacgtcga ggttcaggtc atgaccaacg
      361 gtggagcgga ttttcattac gttaccgcaa gagcagctag cagtaatttc ttcgtatttc
      421 gggtgaatat cttttttcat gggaaaacct cggtttaagg ccgcgtcgct cttccagccc
      481 taacgccaga caccacgcga tgttaaaagt atagcttcaa tacgatcatt tcgtacgaag
      541 gcgcgaaatc atacagaaat taaccagcgt atgcaaactg atccgcactc ttctacggca
      601 atgtgtatac taacccaccg aatttcaagt caggatgatg ctatgcccgt tgcccacgtt
      661 gccttgcccg ttccgcttcc tcgtaccttt gactatctgc tgccagaagg catgacggtt
      721 aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc aggagcgcat cggggttgtg
      781 gtgtcagtta gcgatgtcag tgagctgccg cttaacgagc tgaaagcggt agtggaagtg
      841 ctggacgttg agccggtatt tactcactcc gtctggcggt tactgctctg ggcggcagat
      901 tactatcatc atccgattgg cgatgtgctg tttcatgcct tgccgatttt actgcgccag
      961 gggcggcctg cggcgaacgc gccgatgtgg tactggtttg ccactgaaca aggccaggcg
     1021 gtggatctga acagcctgaa acgctccccc aagcaacaac aggcgctggc ggcgttacgg
     1081 caaggcaaaa tctggcgcga ccaggtcgcc acgctcgaat ttaatgatgc cgcgttgcag
     1141 gcgctacgca aaaaaggtct gtgtgattta gcaagtgaaa caccagagtt tagcgactgg
     1201 caaacgaact atgccgtttc tggtgagcgg ttgcgattga ataccgaaca ggccaccgcc
     1261 gttggcgcaa ttcatagcgc ggcagatact ttttctgcct ggctgctggc gggcgttacc
     1321 ggttccggta aaacggaggt ttatctcagt gtactggaaa acgtgctcgc tcagggcaaa
     1381 caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc aaactatcgc ccgttttcgt
     1441 gaacgtttta atgcccccgt ggaagttctg cattccggcc tgaacgacag cgagcggctt
     1501 tcggcgtggt tgaaagcgaa aaatggcgag gcggcgattg tgatcggcac ccgctccgcg
     1561 ctgtttacgc cgtttaaaaa tctcggcgtg attgtcattg atgaagagca cgacagctcc
     1621 tacaagcagc aggaaggctg gcgctatcat gcccgcgacc tggcggtgta tcgtgcgcat
     1681 agcgagcaaa tcccgattat tcttggctcc gcaacgcccg cgctggaaac gttgtgcaac
     1741 gtccagcaga aaaaataccg cctgctgcgc ctgacgcgcc gggcggggaa tgcgcgtccg
     1801 gcaattcaac acctgctgga tttaaaaggt cagaaggtgc aggcaggtct ggctccggcg
     1861 ttaatcaccc gtatgcgcca gcatttacag gccaacaatc aggttatcct ctttcttaat
     1921 cgtcggggtt ttgcgcctgc gctactgtgc cacgactgcg gctggattgc cgaatgccca
     1981 cgttgcgatc actactacac gctgcatcag gcgcaacagc atttgcgctg tcaccactgt
     2041 gacagtcagc gtccggtgcc gcgccagtgc ccttcctgcg gttccacgca cctggtaccc
     2101 gtggggctgg gcaccgaaca gcttgaacag acgcttgcgc cgctgttccc tgatgtgccc
     2161 atttctcgta tcgaccgcga taccaccagc cgcaaagggg cgctggaaca gcaactggcg
     2221 gaagtacatc gcggcggtgc gaggattttg atcggcacac aaatgctggc gaaaggtcac
     2281 cactttccgg atgtgacgct ggtggcgtta ctggatgtgg acggcgcgct gttttctgcc
     2341 gattttcgct cggcagagcg tttcgctcag ctttacaccc aggtcgctgg tcgtgccggg
     2401 cgtgcgggta aacaaggcga agtggtgctg caaacgcacc atccagaaca tcctctgttg
     2461 caaacgttgc tctataaagg ctacgacgcc tttgccgaac aggcgctggc tgagcgacgg
     2521 atgatgcaac tgccgccatg gaccagccat gtgattgtgc gtgcggaaga tcataacaat
     2581 cagcatgcgc cgctgttcct gcaacaactg cgtaatctga tcctctccag cccgctggca
     2641 gacgacaaac tgtgggttct cggtccggtt ccggcactgg cacctaaacg tggcggtcgc
     2701 tggcgctggc agatattgtt gcagcaccct tcccgcgtgc gcttgcaaca catcattagt
     2761 ggtacgctgg cgctcatcaa tacaataccg gattcccgca aggtgaaatg ggtactggat
     2821 gttgatccga ttgagggtta aaccgcttac gatgcgaggc ggatcgaaaa attcaatatt
     2881 catcacactt ttcatgaaaa ttctgtaacc gttttcacgc gctatctgct aaaaatgttg
     2941 ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc caggcgagga gtgagtgtga
     3001 aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt tgccctcaag gcaaaagtct
     3061 ctacagcgac tgtctcccga gcattaatga atcccgataa agtctcccag gccacccgta
     3121 atcgggttga aaaagcggcc cgggaagtgg gttatttacc gcagcctatg gggcgcaacg
     3181 tcaagcgtaa tgaatcccgc accattctgg tgattgtccc ggatatctgc gatcccttct
     3241 ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa tcacggatat ctggtgctga
     3301 ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt tatcgatttg atcatcacca
     3361 agcaaattga tggcatgttg ctgctgggtt caaggctgcc gtttgatgcc agcattgagg
     3421 aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt cgcaccggaa ctggagctac
     3481 ctacagttca tatcgacaat ctgaccgccg catttgatgc agtaaattat ctatatgagc
     3541 aagggcataa acggattggc tgtatagccg gtcccgaaga gatgccgctg tgtcactacc
     3601 gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat tatggttgat ccgcaataca
     3661 tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc gatgcagcag ctgcttgatc
     3721 ttccacaacc gcctactgct gtcttctgcc atagcgatgt gatggcgctc ggcgcacttt
     3781 ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct ttccataatc ggttttgata
     3841 acatcgacct gacgcaattt tgtgatccgc cgctgacaac catcgcgcag ccgcgttacg
     3901 aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat gcaggggcaa cacgttggca
     3961 gtggctctcg tttaatggac tgcgaactta tcatccgggg atcaacacgc gcgttacctt
     4021 aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc gccgcttaag taacatggcg
     4081 ggctgacgaa cgaataaata cagcgaaacg atagtggcac aacgagatta tgtacgccgc
     4141 agccaaccgg caccttcgcg gcgaaaaaag agcacctcac ggaaaaagca acgaaatctg
     4201 cctgcggttt ctcccgctat ggtcgctatt gctgccgccg ttcttgtgac ctttatcggt
     4261 ggtctgtact tcattacgca tcacaagaaa gaagagtccg agacgctgca aagccagaaa
     4321 gtgaccggta acggactacc accaaaacca gaagaacgct ggcgctacat taaagagctg
     4381 gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt ctgccggtgg tgaagtgaaa
     4441 acgccggagc aactgacacc agaacaacgt cagcttcttg aacaaatgca ggctgatatg
     4501 cgccagcagc caacgcagct ggttgaagtt ccgtggaatg aacagacgcc ggaacagcgt
     4561 cagcaaacgc tacaacgcca gcgtcaggcg cagcaattag cggagcagca acgcctggct
     4621 cagcagtcgc gtacgactga acaaagctgg cagcagcaga cgcgtacgtc gcaagccgcg
     4681 ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta ctcagcagcc gtaccaggat
     4741 ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc cgcagcaagc tgcgccggtt
     4801 gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa aagacgaacg ccgctggatg
     4861 gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga cggtacgtgc tcagctggcg
     4921 ttcgaaggat ttgactcgaa aatcaccacc aacaatggct ggaatcgtgt ggtcattggc
     4981 ccggtgaaag gcaaagagaa cgcagacagc accctcaatc ggttgaagat ggcgggtcat
     5041 acaaactgca ttcggctcgc cgccgggggt tgaaaccctc aaaatccccc ccatctataa
     5101 ttgcattatg ccccgtactt ttgtacgggg tttgtactct gtattcgtaa ccaaggggtc
     5161 agctcgtgac aactatagta agcgtacgcc gtaacggcca tgtggtcatc gctggtgatg
     5221 gtcaggccac gttgggcaat accgtaatga aaggcaacgt gaaaaaggtc cgccgtctgt
     5281 acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc ggatgctttt acgctgttcg
     5341 aactgtttga acgtaaactg gaaatgcatc agggccatct ggtcaaagcc gccgttgagc
     5401 tggcgaaaga ctggcgtacc gatcgcatgc tgcgcaaact tgaagcactg ctggcagtcg
     5461 cggatgaaac tgcatcgctt atcatcaccg gtaacggtga cgtggtgcag ccagaaaacg
     5521 atcttattgc tatcggctcc ggcggccctt acgcccaggc tgcggcgcgt gcactgttag
     5581 aaaatactga actgagtgct cgtgaaattg ctgaaaaggc gttggatatt gcaggcgaca
     5641 tttgcatcta taccaaccat ttccacacca tcgaagaatt aagctacaaa gcgtaaggat
     5701 ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga actggataag cacatcatcg
     5761 gccaggacaa cgccaagcgt tctgtggcga ttgctctgcg taaccgctgg cgtcgcatgc
     5821 agctcaacga agagctgcgc catgaagtga ccccgaaaaa tatcctgatg atcggcccga
     5881 ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa gctggcgaat gcgccgttca
     5941 tcaaagttga agcgaccaaa ttcaccgaag tgggctacgt cggtaaggaa gtggattcta
     6001 ttattcgcga tctgaccgat gccgccgtga aaatggtacg cgtccaggct atcgagaaaa
     6061 accgttatcg cgctgaagaa ctggcagaag aacgtattct cgacgtgctg atcccacctg
     6121 ctaaaaacaa ctggggacag accgaacagc agcaggaacc gtccgctgct cgtcaggcat
     6181 tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga aatcgagatc gatcttgccg
     6241 cagcaccgat gggcgttgaa attatggctc ctccaggcat ggaagagatg accagccagc
     6301 tgcagtccat gttccagaac ctgggcggcc agaagcaaaa agcgcgtaag ctgaaaatca
     6361 aagacgccat gaagctgctg attgaagaag aagcggcgaa actggtgaac ccggaagagc
     6421 tgaagcaaga cgctatcgac gctgttgagc agcacgggat cgtgtttatc gacgaaatcg
     6481 acaaaatctg taagcgcggc gagtcttccg gtccggatgt ttctcgtgaa ggcgttcagc
     6541 gtgacctgct gccgctggta gaaggttgca ccgtttccac caaacacggg atggtcaaaa
     6601 ctgaccacat tctgtttatc gcttctggcg cgttccagat tgcgaaaccg tctgacctga
     6661 tcccggaact gcaaggtcgt ctgccaatcc gcgttgaact gcaggcgctg accaccagcg
     6721 acttcgagcg tattctgacc gagccgaatg cctctatcac cgtgcagtac aaagcactga
     6781 tggcgactga aggcgtaaat atcgagttta ccgactccgg tattaagcgc atcgcggaag
     6841 cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc tcgtcgttta cacactgttc
     6901 tggagcgttt aatggaagag atttcctacg acgccagcga tttaagcggt caaactatca
     6961 tcattgacgc agattatgtg agcaaacatc tggatgcgtt ggtggcagat gaagatctga
     7021 gccgttttat cctataatcg cgttcaatca ttttcatcat tgtttgatgg ggctgaaagg
     7081 ccccattttt attggcgcgt attatgactg aacaacaaat tagccgaact caggcgtggc
     7141 tggaaagttt acgacctaaa accctccccc tcgcctttgc tgcaattatc gtcgggacag
     7201 cgctggcatg gtggcaaggt cacttcgatc cgctggtcgc cctgctggca ctgattaccg
     7261 ccgggctatt acagatcctt tctaacctcg ccaatgatta cggcgatgcg gtaaaaggca
     7321 gcgataaacc tgaccgcatt gggccgctac gcggcatgca aaaaggggtc attacccagc
     7381 aagagatgaa acgggcgctc attattaccg tcgtgctcat ctgtctctcc gggctggcac
     7441 tggttgcagt ggcatgccat acgctggccg attttgtcgg tttcctgatt cttggcgggt
     7501 tgtcgatcat tgccgctatc acctacaccg tgggcaatcg tccttatggt tatatcggtc
     7561 tgggtgatat ttccgtactg gttttctttg gctggttgag tgtcatgggg agctggtatt
     7621 tacaggctca tacattgatt ccggcactga tccttccggc gaccgcatgc ggcctgctgg
     7681 caacggcagt actgaatatt aataacctgc gtgatatcaa tagcgaccgc gaaaatggca
     7741 aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg tcgttatcat gcctgcctgc
     7801 tgatgggctc gctggtgtgt ctggcgctgt ttaatctctt ttcgctgcat agcctgtggg
     7861 gctggctgtt cctgctggcg gcaccattac tggtgaagca agcccgttat gtgatgcggg
     7921 aaatggaccc ggtggcgatg cgaccaatgc tggaacgtac tgtcaaggga gcgttactga
     7981 ctaacctgct gtttgtttta gggatattcc taagccagtg ggcagcataa ctgacaaata
     8041 tcaattaaca attgatgatt ttgccaacag cccacatagc gcgatatact gaaaattctc
     8101 gcagcaactg aatgttaagc ctatgaaata cgatacttcc gagctttgtg acatctatca
     8161 agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt ggcggacggg cgtcgtttgg
     8221 cggacaaata atcacggtaa aatgtttcga ggacaacggg ttgctgtacg atctgctcga
     8281 acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt ggttctgttc gtcgcgcact
     8341 ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa tgggaaggtc tggtcattta
     8401 cggcgcggtg cgtcaggtag atgacctgga agagttggat atcggcatcc aggcgatggc
     8461 ggcaattccg gttggtgccg ctggcgaagg cattggcgaa agcgatgtcc gcgtcaattt
     8521 tggcggtgtc accttcttct ccggcgacca tctttatgcc gacaataccg ggattattct
     8581 ttctgaagat ccgctggata ttgaatgata gaaaaggcac cgcaaggtgc cttttttctg
     8641 cgttacctgt tggcctacac agtaaagaaa ttacgcggaa gatgaagcgt aatcagacct
     8701 cttccatgcg acccagcagg gcctgcagac gttcctgcca gccgttctgc tgttctttca
     8761 gatggttgtt ctcacgctcc agctcttcgc gctgatgctg ggcattttga acttcctgcg
     8821 acagtgagtt gtttttttct ttcagctctt cgatttccat ctgcaacaga gtgatggtat
     8881 caatcgcctg ctgtactttt gcttccagtt tctcaaacac ttctaatgac attgtcatac
     8941 ctctcctgaa ttgcaaggcg ttgatggata aaaatcctcg tcccgattac cggtgacgcc
     9001 ttaataaata cgagcgcact ttagttagct ccgattgtat gaagccgcgc catcgctgtc
     9061 cagcggcacg ccttgcagat tacggtttgc cacacttttc atccttctcc tggtgacata
     9121 atccacatca atcgaaaatg ttaataaatt tgttgcgcga atgatctaac aaacatgcat
     9181 catgtacaat cagatggaat aaatggcgcg ataacgctca ttttatgacg aagcacacac
     9241 attttaagtt cgatatttct cgtttttgct cgttaacgat aagtttacag catgcctaca
     9301 agcatcgtgg aggtccgtga ctttcacgca tacaacaaac attaactctt caggatccga
     9361 ttatgagtca aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc ggtaccgggt
     9421 tgttgatttt cttcggtgtg ggttgcgttg cagcactaaa agtcgctggt gcgtcttttg
     9481 gtcagtggga aatcagtgtc atttggggac tgggggtggc aatggccatc tacctgaccg
     9541 caggggtttc cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg ctgtttgcct
     9601 gtttcgacaa gcgcaaagtt attcctttta tcgtttcaca agttgccggc gctttctgtg
     9661 ctgcggcttt agtttacggg ctttactaca atttattttt cgacttcgag cagactcatc
     9721 acattgttcg cggcagcgtt gaaagtgttg atctggctgg cactttctct acttacccta
     9781 atcctcatat caattttgtg caggctttcg cagttgagat ggtgattacc gctattctga
     9841 tggggctgat cctggcgtta acggacgatg gcaacggtgt accacgcggc cctttggctc
     9901 ccttgctgat tggtctactg attgcggtca ttggcgcatc tatgggccca ttgacaggtt
     9961 ttgccatgaa cccagcgcgt gacttcggtc cgaaagtctt tgcctggctg gcgggctggg
    10021 gcaatgtcgc ctttaccggc ggcagagaca ttccttactt cctggtgccg cttttcggcc
    10081 ctatcgttgg cgcgattgta ggtgcatttg cctaccgcaa actgattggt cgccatttgc
    10141 cttgcgatat ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa caaaaagctt
    10201 cgctgtaata tgactacggg acaattaaac atgactgaaa aaaaatatat cgttgcgctc
    10261 gaccagggca ccaccagctc ccgcgcggtc gtaatggatc acgatgccaa tatcattagc
    10321 gtgtcgcagc gcgaatttga gcaaatctac ccaaaaccag gttgggtaga acacgaccca
    10381 atggaaatct gggccaccca aagctccacg ctggtagaag tgctggcgaa agccgatatc
    10441 agttccgatc aaattgcagc tatcggtatt acgaaccagc gtgaaaccac tattgtctgg
    10501 gaaaaagaaa ccggcaagcc tatctataac gccattgtct ggcaccaaag tgaagtggat
    10561 cctcgaccat gtggaaggct ctcgcgagcg tgcacgtcgt ggtgaattgc tgtttggtac
    10621 ggttgatacg tggcttatct ggaaaatgac tcagggccgt gtccatgtga ccgattacac
    10681 caacgcctct cgtaccatgt tgttcaacat ccataccctg gactgggacg acaaaatgct
    10741 ggaagtgctg gatattccgc gcgagatgct gccagaagtg cgtcgttctt ccgaagtata
    10801 cggtcagact aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg
    10861 tgaccagcag gccgcgctgt ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac
    10921 ctatggcact ggctgcttta tgctgatgaa cactggcgag aaagcggtga aatcagaaaa
    10981 cggcctgctg accaccatcg cctgcggccc gactggcgaa gtgaactatg cgttggaagg
    11041 tgcggtgttt atggcaggcg catccattca gtggctgcgc gatgagatga agttgattaa
    11101 cgacgcctac gattccgaat atttcgccac caaagtgcaa aacaccaatg gtgtgtatgt
    11161 ggttccggca tttaccgggc tgggtgcgcc gtactgggac ccgtatgcgc gcggggcgat
    11221 tttcggtctg actcgtgggg tgaacgctaa ccacattata cgcgcgacgc tggagtctat
    11281 tgcttatcag acgcgtgacg tgctggaagc gatgcaggcc gactctggta tccgtctgca
    11341 cgccctgcgc gtggatggtg gcgcagtagc aaacaatttc ctgatgcagt tccagtccga
    11401 tattctcggc acccgcgttg agcgcccgga agttcgcgaa gtgactgcat tgggtgcggc
    11461 ctatcttgct ggtctggcgg tcggtttctg gcagaacctc gacgagctgc aagagaaagc
    11521 ggtgattgaa cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt accgttacgc
    11581 aggctggaaa aaagcggtca aacgcgcgat ggcgtgggaa gaacacgacg agtaatgtaa
    11641 atgccggatg aagcgtttat gccgcatccg gtagtcctga aaccgtgcgg gggcaacccc
    11701 gcacacatca ataatcccac ccttcccctg tgctacactt cgcgccattc cttactgctt
    11761 agagtttgct atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatctgcggc
    11821 gctggctggc tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt
    11881 aaacgccatg cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga
    11941 aggtgaaatc gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg
    12001 cgacgcggta gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca
    12061 ggctaacgcg ctggcggtgc tggcagtagg cgataaaggc tgcttcctca atgcgccgga
    12121 tatgtatatg gagaagctga ttgtcgggcc gggagccaaa ggcaccattg atctgaacct
    12181 gccgctggcg gataacctgc gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact
    12241 gacggtaacg attctagcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact
    12301 cggcgtacgc gtatttgcta ttccggatgg cgacgttgcg gcctcaattc tcacctgtat
    12361 gccagacagc gaagttgacg tgctgtacgg tattggtggc gcgccggaag gcgtagtttc
    12421 tgcggcggtg atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga
    12481 cgtcaaaggc gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa
    12541 agcgatgggc atcgaagccg gtaaagtatt gcgcctgggc gatatggcgc gcagcgataa
    12601 cgtcatcttc tctgccaccg gtattaccaa aggcgatctg ctggaaggca ttagccgcaa
    12661 aggcaatatc gcgactaccg aaacgctgct gatccgcggc aagtcacgca ccattcgccg
    12721 cattcagtcc atccactatc tggatcgcaa agacccggaa atgcaggtgc acatcctctg
    12781 attgatttga tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa
    12841 cgaagataag gcaagttaat caaaacagga gaaaaacatg gctgattggg taacaggcaa
    12901 agtcactaaa gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt
    12961 gcttccgttt accgccgggc aattcaccaa gcttggcctt gaaatcgacg gcgaacgcgt
    13021 ccagcgcgcc tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt
    13081 caccgtcccc gatggcaaat taagcccacg attggcggca ctgaaaccag gcgatgaagt
    13141 gcaggtggtt agcgaagcag ctggcttctt tgttctggat gaagtaccgg actgcgaaac
    13201 gctatggatg ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg
    13261 caaagattta gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga
    13321 cttaagctat ttgccactga tgcaagaact ggaaaaacgc tacgaaggga aactgcgcat
    13381 tcagacggtg gtcagtcggg aaacggcagc ggggtcgctc accgggcgga taccggcgtt
    13441 aattgaaagt ggggaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca
    13501 tgtgatgctg tgcggcaatc cacagatggt gcgcgataca caacagttgc tgaaagagac
    13561 ccggcagatg acgaaacatt tacgtcgccg accgggccat atgacagcgg agcattactg
    13621 gtaagcggtt acttatcgat aaacggcacg atgagcaaat ccgcactcat cttattgatc
    13681 atcccgcgat atgccggcat caaacggttg ataaatgagt gatgatgacc acagacaagg
    13741 aggtcgcact gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca
    13801 atacgcagtt ttgtcttcgg ccattgaata tttttcgtca gtttatacag cttgttatcc
    13861 gacttattct tcaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac
    13921 aactcgctta agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg
    13981 agctccagag ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca
    14041 atgtgtttat aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca
    14101 gtgataactc aaaaataatc atctcggcct ggcatgagaa agtgaaggcc gcatcaagct
    14161 caacttgccc ctccagatcc ttaatcttgt aggccacgct ggccccttca tctgcagagg
    14221 atgcagcggc ggcaagctct ttcagtttgg tcataaaaga ctctgcttct tcgcgggtgg
    14281 caaaaaagcg cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa
    14341 tcgcacatcc tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat
    14401 tcgcctctct gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt
    14461 aatgatctta tggattacca ttttttttga tattgcttaa ggtgaagagt tcagaaggat
    14521 tttccgggca ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag
    14581 agatatatta aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgggta
    14641 tctttaccat atttattttc gccttgtgtg ccaatgaacg ctccaagatc gataagcatc
    14701 atcaccagaa tcaacgtcgg gacaaaacgc cccaccgccc attgccagac acccggtaaa
    14761 atcgcccagt tacccgccag cagcatccac gccacaatca tcagaaatgc ccatgcgccg
    14821 gaacgcccgc gatcatgcaa gcgcttaacg gttactgccg ctgttggcca aagcaagcac
    14881 acaaggcaaa acgccgcggt ctgaatatcg agtaaatttt tacccgccag tgagaaaagc
    14941 accagcatgc ctgcgaacca caggcctatc caaatccaga aatcacggcg tccaatacgc
    15001 cctttaaatg agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt
    15061 ccaggattct acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa
    15121 caaaaggtac tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg ttctgcatta
    15181 accgttacaa caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg
    15241 ctggctggag cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc
    15301 cagaatccca gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc
    15361 aatctcactc gtgctgcaag taaaattaac gagaacttgt atgcttctac agcgctggag
    15421 cgcggtacct taaaaatcaa aagcattcaa atgacctggc tacccatcca ggggccagag
    15481 caaaaagcag cgaaagcgaa agctcaggaa tacatggcag cagtgatccg cacactcacc
    15541 ccattaatga ccaaaacaca aagccagaaa aaactgcagt cgctgctaac ggcggggaaa
    15601 aacaaacgtt attacaccga gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc
    15661 gaaaaggggc tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa
    15721 ggtttgaata aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac
    15781 agacctgctg ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta
    15841 aaatgcgaca tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc
    15901 acgagctggt ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa
    15961 tcgcaccacc ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc agccacatca
    16021 tgctgggtgc gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacttctg
    16081 ccgctatgct gaaagacatc ggcgctcagt acatcatcat cggccactct gaacgtcgta
    16141 cttaccacaa agagtctgac gaactgatcg cgaaaaaatt cgcggtgctg aaagagcagg
    16201 gcctgactcc ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggcaaaactg
    16261 aagaagtttg cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg
    16321 aaggtgcggt tatcgcttac gaacctgtat gggcaatcgg tactggcaaa tctgcaactc
    16381 cggctcaggc acaggctgtt cacaaattca tccgtgacca catcgctaaa gttgacgcta
    16441 acatcgctga acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag
    16501 aactgtttgc tcagccagat atcgacggcg cgctggttgg cggtgcttct ctgaaagctg
    16561 acgctttcgc agtaatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg
    16621 tgccggattt catatccggc acttactttc cttaactctt cgccttaacg caaaatctca
    16681 cactgatgat cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag atttcgttgt
    16741 gtcgccagca atacaaatga gttatcactc tgccgtacca tcgccagccc gtagcttccc
    16801 atatgttccc gcgcctcagg tacttcttct gccagcatca taaatgggct gcgttgtacc
    16861 agttcgcttt ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt
    16921 ggcaaccagc ggctgctgat gttcgccaga ttgttatcca gttgttcgcg cacatcagga
    16981 cgaatacaag agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc
    17041 gccaaagaaa ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc
    17101 gcctgccagg ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca
    17161 gtaccgttaa tacgatacgt tggcatcaac agatattgca gtggaccatt aagatctttt
    17221 aaaaccacgt atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc
    17281 tgctgattgg gcaaacattc ctcaaggaca atcttacgta atgtatccga ctcttcaccg
    17341 gttaatttcc agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa
    17401 agaagacccg cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc
    17461 aaaatcgtga caaataaaaa acccggtcac gagaaaccac cgggtttgat aattatcctg
    17521 gggaaatcag cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg
    17581 cgctttcgtc cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc
    17641 attttcgtac tttttcgcca cctcagcgtc gcgcggacgg tagtagtttt tcgcggcaat
    17701 ttcctgacct tctggcgagt agagatattt caggtaggct tccgccacct ctttggtgcc
    17761 ttttttctcg accactttat cgaccaccga cacggttggc tctgcgagga tagactcact
    17821 cggcgtgacg atttcgaatt tatctttccc cagttcattc gctgccagca gagcttcgtt
    17881 ttcccaggcg atcagtacat cgccaatccc gcgctcgaca aaggtgttag tggagccgcg
    17941 cgcgccagaa tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt
    18001 tgcctgatcg ttgttgttgt gatgcagcgc gtagccccag gcagccaggt agttccagcg
    18061 tgcgccacca gagcttttcg gattaggcgt gatcaccgaa acacccggtt taatcagatc
    18121 gttccagtca tggatctgct tcggattgcc cttacgcacc aggaaaacaa tggtggaagt
    18181 gtacggtgcg gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc
    18241 cgcaattgcg tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt
    18301 gattaccgac gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca ccacgttatc
    18361 accagtttgc tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg
    18421 cgttggatca tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc
    18481 cagcaaaaat gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt
    18541 gtgatgagca aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct
    18601 ataactttcg ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc tcatattgct
    18661 gacaaagtgc gctttgtcca tgccggatgc ggcgcgaacg ccttatccgg cctacaaaag
    18721 tttgcaaatt caataaattg cagaattcat gtaggcctga taagcgaagc gcatcaggca
    18781 tttttgcttc tgtcatcggt ttcagggtaa aggaatctgc ctttttccga aatcattaat
    18841 acagtttttt cgcgcagtcc agccagtcgc ctttgaacgg acgcttcatg ttttcgatag
    18901 cgtcgatgat gtcgtggtga accaactgtt cgttctggat acctacgcaa cgaccgccgt
    18961 aacctgccag cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt
    19021 aaggcaccgg agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac
    19081 cggtttcttt ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg
    19141 ccacgatcgc gtgtttttta cctttcgcga tacccgcttt gatttcgttt accaggtctt
    19201 cacggctgaa ttcaacttcc ggaaccacaa cgaattcaca gccaccggca atggccgcag
    19261 ccagggtcag atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag
    19321 aagaagaggt gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga
    19381 aaccgatagt gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc agaccgatgc
    19441 acgggaagcc catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa
    19501 ccaccagcgc gtcgatacca cgttttttca ggttttcgat agccacggcg cggatgttct
    19561 cgtcgcggaa ttccgggaaa cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt
    19621 cagacacgct gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt
    19681 cataaatacc cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg
    19741 cggcgttcat gcctggcgca tcaccgccgc ttgtcaacac accgattttc ttaatcatga
    19801 ctacctctga actttggaat gcaaaatgaa atctgttgcc ggaagtcttc ttgcacatcg
    19861 aagtgatcca acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt
    19921 caggccaaat ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt
    19981 atgaaagcat agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa
    20041 ttacatccga tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat
    20101 gtgcctgaac caaaggcaga gagtcttcca tttccaaatg aatctgaata aagcgggtcg
    20161 gccctgactg ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga
    20221 tatcaataat ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat gactgtaccg
    20281 cctcatatcc catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca
    20341 gagcatcggc gcgatgccag ccgtaccagg acaaccccag cgccagcaga attgcgccgt
    20401 tcatcataac atcagactgg taatgtagca tatcagcccg caccgcctgg ctttgcgtcc
    20461 gccgcaccac ccaacgctga aacgagacaa ggataatcgt acaaattagc gccacaattg
    20521 tcacgataac cccgacgcct ggatctgtca tcggtgttgg agatatcaga tgttgaatac
    20581 ccgtcaaaaa caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga
    20641 gggactcagc tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc
    20701 gcaccaccaa taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg
    20761 cgagaatact caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcagcg
    20821 aagccatcgc cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca
    20881 taaatactcc cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt
    20941 aagcagcagg caaattgagg ataaaaaaaa cccccacatc atgtggggga agacagggat
    21001 ggtgtctatg gcaaggaaaa cagggtttac tactgggaac gtgagttgct actactcaat
    21061 agcttcaacg atgaactttt ttgccattgc gtcacgtcac gcaactgctc cattcgttgt
    21121 tgatgtttct cgtttaaaac cgcttgctgc tccggcgtta acaggcgata catttggttg
    21181 cggacttttg ccatctcaac ctgacgagca atttgctcat tcgccatttt ttctgcctgt
    21241 gcgcgcacag cgttttcatc aaaattttct gcggtgacaa ggcgatgcat tgtctccagt
    21301 tcgctaacat taacaggagg ctgttcgtgc cgggcctgtt gcataagatc tcgcatctgc
    21361 tgacgctgat gttcggttaa acttatgccg tcgaacatat ggctctgcgt actgcgctgc
    21421 gtaagttctt cacccggatg ccagttatcg cctgaaccga cttcagcagc gtggcttaat
    21481 gaactgactg ccagcgttga ggccatgacg gcagcggtaa ctatgcgcat catttgctcc
    21541 caaaatcttt ctgtcgcgat tcaacgatag agagtttacg attcaggctg caaacatgcg
    21601 tcagggggtg taaaacaacg taaagtcatg gattagcgac gtctgatgac gtaatttctg
    21661 cctcggaggt atttaaacaa tgaataaaat cctgttagtt gatgatgacc gagagctgac
    21721 ttccctatta aaggagctgc tcgagatgga aggcttcaac gtgattgttg cccacgatgg
    21781 ggaacaggcg cttgatcttc tggacgacag cattgattta cttttgcttg acgtaatgat
    21841 gccgaagaaa aatggtatcg acacattaaa agcacttcgc cagacacacc agacgcctgt
    21901 cattatgttg acggcgcgcg gcagtgaact tgatcgcgtt ctcggccttg agctgggcgc
    21961 agatgactat ctcccgaaac cgtttaatga tcgtgagctg gtggcacgta ttcgcgcgat
    22021 cctgcgccgt tcgcactgga gcgagcaaca gcaaaacaac gacaacggtt caccgacact
    22081 ggaagttgat gccttagtgc tgaatccagg ccgtcaggaa gccagcttcg acgggcaaac
    22141 gctggagtta accggtactg agtttaccct gctctatttg ctggcacagc atctgggtca
    22201 ggtggtttcc cgtgaacatt taagccagga agtgttgggc aaacgcctga cgcctttcga
    22261 ccgcgctatt gatatgcaca tttccaacct gcgtcgtaaa ctgccggatc gtaaagatgg
    22321 tcacccgtgg tttaaaacct tgcgtggtcg cggctatctg atggtttctg cttcatgata
    22381 ggcagcttaa ccgcgcgcat cttcgccatc ttctggctga cgctggcgct ggtgttgatg
    22441 ttggttttga tgttacccaa gctcgattca cgccagatga ccgagcttct ggatagcgaa
    22501 cagcgtcagg ggctgatgat tgagcagcat gtcgaagcgg agctggcgaa cgatccgccc
    22561 aacgatttaa tgtggtggcg gcgtctgttc cgggcgattg ataagtgggc accgccagga
    22621 cagcgtttgt tattggtgac caccgaaggc cgcgtgatcg gcgctgaacg cagcgaaatg
    22681 cagatcattc gtaactttat tggtcaggcc gataacgccg atcatccgca gaagaaaaag
    22741 tatggccgcg tggaactggt cggtccgttc tccgtgcgtg atggcgaaga taattaccaa
    22801 ctttatctga ttcgtccggc cagcagttct caatccgatt tcattaactt actgtttgac
    22861 cgcccgctat tactgctgat tgtcaccatg ttggtcagta cgccgctgct gttgtggttg
    22921 gcctggagtc tggcaaaacc ggcgcgtaag ctgaaaaacg ctgccgatga agttgcccag
    22981 ggaaacttac gccagcaccc ggaactggaa gcggggccac aggaattcct tgccgcaggt
    23041 gccagtttta accagatggt caccgcgctg gagcgcatga tgacctctca gcagcgtctg
    23101 ctttctgata tctctcacga gctgcgcacc ccgctgacgc gtctgcaact gggtacggcg
    23161 ttactgcgcc gtcgtagcgg tgaaagcaag gaactggagc gtattgaaac cgaagcgcaa
    23221 cgtctggaca gcatgatcaa cgatctgttg gtgatgtcac gtaatcagca aaaaaacgcg
    23281 ctggttagcg aaaccatcaa agccaaccag ttgtggagtg aagtgctgga taacgcggcg
    23341 ttcgaagccg agcaaatggg caagtcgttg acagttaact tcccgcctgg gccgtggccg
    23401 ctgtacggca atccgaacgc cctggaaagt gcgctggaaa acattgttcg taatgctctg
    23461 cgttattccc atacgaagat tgaagtgggc tttgcggtag ataaagacgg tatcaccatt
    23521 acggtggacg acgatggtcc tggcgttagc ccggaagatc gcgaacagat tttccgtccg
    23581 ttctatcgga ccgatgaagc acgcgatcgt gaatctggcg gtacaggatt ggggctggcg
    23641 attgttgaaa ccgccattca gcagcatcgt ggctgggtga aggcagaaga cagcccgctg
    23701 ggcggtttac ggctggtgat ttggttgccg ctgtataagc ggagttaaac tccgcatttg
    23761 taggcaggat aaggcgttta cgccgcatcc ggcatttgag caggatgcct gatgcgacgc
    23821 tgatagcgtc ttatcaggcc tacactccgg gggttatttt ccccacaacc ggcgggaaaa
    23881 atcttcgatc ttgccgctca gtcggcgctt ttgcatcgtc cgcgtccagc ttttcgataa
    23941 cccggcggcg gagagtaaac ggtgatattg atcgtcatca aacggcatat gccatgcgat
    24001 ggcggcagcc tcctgcacgg tcacatcgct gacacgggaa accaactcca gcggcgcgtc
    24061 cgcagacact ttccctggcg ctatcacgct atacaaccac cccaccttac ctgtgttttg
    24121 catcaactgc gctatatcgc tgatatcaaa atggtaattg agtttatagc aaggcgagcg
    24181 cggctggctg acctgaatta atgcctctcc ccagcggaaa atatcgccca tataaacatt
    24241 actttccgtc aggccgtcgg ttgagaggtt ttcaccaaac gcaggcgcaa caaacaactc
    24301 cgcctgttcc ggaaattccc gcgcccagta gagataatgc tcacgaggat aatgacacag
    24361 cgctctgtct ggcccgccgt gaaccttttt ctccgcctgc tcgtcacctt ccagccccag
    24421 ctccgtcagc atcaactcac catcaacctg gattttggca attgcgctgg gtttgccttc
    24481 gggataagcc tgaatcttgc ctgtgtatac atcaaccgga tatcgcatct gttccctctc
    24541 cacgagaaat ttccccatta gaccatattt attttatgtc aggcgtgctg ttttcgcggt
    24601 aagtatccag gcaggaactc aatagcaaac cggacgaacg ttagttcatc cggctgtggg
    24661 gattaaggaa gcaggtttat ttcaccgtac ctaaaatttc gattttcgct gctctggctt
    24721 tgactttacg tgaccagata gaggtaagaa ttggcaccag aatcgaagtc acaatgaccg
    24781 ccgtcgctac cagcgaagtt gctgccggag ccatcggttt aaacgcaggc accatttctg
    24841 caatcagcac aggtgtcgct accgcggccc ctgcggaact ggaagcggca attccggcag
    24901 tgccatcgcc accgccaatc aatttatcgg cgataatcaa cggaataccg gtcacgataa
    24961 ttactgccac acccaacagg atccccagca aaccagtctg ggcaatcaca gtcaaatcaa
    25021 tggtattgcc cagcgcgaag gcaaagaatg gaatcagcgt ttgcaccgct ttgctgaaaa
    25081 attctcgtaa ttcagggtca aggttcccca gggcaaagcc caccaggaac ggtaatactg
    25141 cgccgacgaa aacatgcggt tcaaacgagg caatccctgc agtgcccaga ataatcatcg
    25201 tcatgagcgg cccggactcc aacgacatca acacaaatgc cccagcttct tcttttgtgc
    25261 cgtactgctg catgatggaa gcgtaaagtc cgccattggt catatccatc gccgccacca
    25321 gcgccagcgt tgaaagtccg gcaaagaatc caacttcaac accatgttcc ggaataatgc
    25381 gcgaggcaat tgccgcaacc acccacgcga cggcaatttt tgttaccacc agcgtaccgg
    25441 atttacgcaa taccgttccc gtcgcgctta attttattga cgcccccatg caaaaaaacc
    25501 acaccgccag aatgggcacc gtaccggtaa tcatcccgtt ggtgaatgat ccaaaatatt
    25561 tccccgcccc cggcgagaag gtgtggcaca gtgcgccaag gaatagcggg acgagcatca
    25621 tcccccccgg gattttctca atcgagcgtt ttatctgcat ttccatcacc tgtcggcata
    25681 tttttaaggg ctgatgcgta attcctcaat ggagcatcag tgttgatgga aacgaagata
    25741 gacattcatt taatcaataa aagtgatcaa aaccacatca attgaaacac tgttttattt
    25801 ttatcggatc attgttttta ttttgaaact gatcacaaaa aaaccaccgc cgttggcgat
    25861 ggttcatttt ttaagctgat atgcggcgta tacgcctcat tgcagcaggc ggcaaataat
    25921 tattttttcg ccgcgaaacg tgccgatgct tcgtcccagt tcaccacgtt ccagaactct
    25981 ttgatgtagt ccggacggcg gttctggaat ttcaggtagt aagcatgttc ccacacatcc
    26041 aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt cacccatcag cggagaatcc
    26101 tggttagcag tagaaaccac cgccagttta tcgcctttca gcaccagcca tgcccagccg
    26161 gaaccaaagc gggaagctgc cgctttctca aattctgctt tgaagttatc aacggagccg
    26221 aagtcacgtt cgatagccgc tttcaggtca ccctgcaggg tggtgccttt tttcagacct
    26281 ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt tgcgcagtac ggttttcttg
    26341 tctgctggca gctggtccag tttggtgatc agctcttcaa ccggcaggtt ggcaaattct
    26401 ggcaggcttt ccagcgccgc gttggcgttg ttgacgtagg tctgatggtg tttggtgtgg
    26461 tggatttcca tggtctgctt atcgaagtgc ggttccaggg catcgtaagc atacggcagg
    26521 gatggcaggg tatagctcat attcatctcc agtattgtcg ggcggccgat tgttaatgcc
    26581 gcgtaagcag ttggttcatt atagttaatt aaatgatatt gaaaatgatt atcaatgccg
    26641 tacttttcgt aagggtatgg ttttgcagga aaatgcccga gatgtgaagc aaatcaccca
    26701 cttaatgccg tgattgccag taaatcgaca acggcggcaa caggcgaaag gttaatcgac
    26761 agcacgattt ttacactcat ctcgtcggag atgtgacgcg acgaaaaatg atgaggataa
    26821 gaatatgagt aacgcgatta cgatggggat attttggcat ttgatcggcg cggccagtgc
    26881 agcctgtttt tacgctccgt tcaaaaaagt aaaaaaatgg tcatgggaaa ccatgtggtc
    26941 agtcggtggg attgtttcgt ggattattct gccgtgggcc atcagcgccc tgttactacc
    27001 gaatttctgg gcgtattaca gctcgtttag tctctctacg ctgctgcctg tttttctgtt
    27061 cggcgctatg tgggggatcg gtaatatcaa ctacggcctg accatgcgtt atctcggcat
    27121 gtcgatggga attggcatcg ccattggcat tacgttgatt gtcggtacgc tgatgacgcc
    27181 aattatcaat ggcaatttcg atgtgctgat taacaccgaa ggcggacgca tgacgttgct
    27241 cggcgttctg gtggcgctga ttggcgtagg gattgtaact cgagccgggc agttgaaaga
    27301 gcgcaagatg ggcattaaag ccgaagagtt caatctgaaa aaagggctgg tgctggcggt
    27361 gatgtgcggc attttctctg ccgggatgtc ctttgcgatg aacgccgcaa aaccgatgca
    27421 tgaagccgct gccgcactag gcgtcgatcc actgtatgtc gctctgccaa gctatgtcat
    27481 catcatgggc ggcggcgcga tcatcaacct cggtttctgt tttattcgtc tggcaaaagt
    27541 gaaggatttg tcgctaaaag ccgacttctc gctggcaaaa ccgctgatca ttcacaatgt
    27601 gttactctca gcactgggcg ggttgatgtg gtatctgcaa ttctttttct atgcctgggg
    27661 ccacgcccgc attccggcgc agtatgacta catcagttgg atgctgcata tgagtttcta
    27721 tgtattgtgc ggcggtatcg tcgggctggt gctgaaagag tggaacaatg caggtcgcca
    27781 tccggtaacg gtgttgagcc tcggttgtgt ggtgattatt gtcgccgcca acatcgtcgg
    27841 catcggcatg gcgaattaat ctttctgcga attgagatga cgccactggc tgggcgtcat
    27901 cccggtttcc cgggtaaaca ccaccgaaaa atagttacta tcttcaaagc cacattcggt
    27961 cgaaatatca ctgattaaca ggcggctatg ctggagaaga tattgcgcat gacacactct
    28021 gacctgccgc agatattgat tgatggtcat tccagtctgc tggcgaaatt gctgacgcaa
    28081 aacgcgctca ctgcacgatg cctcatcaca aaatttatcc agcgcaaagg gacttttcag
    28141 gctagccgcc agccgggtaa tcagcttatc cagcaacgtt tcgctggatg ttggcggcaa
    28201 cgaatcactg gtgtaacgat ggcgattcag caacatcacc aactgcccga acagcaactc
    28261 agccatttcg ttagcaaacg acacatgctg actactttca tgctcaagct gaccgataac
    28321 ctgccgcgcc tgcgccatcc ccacgctacc taagcgccag tgtggttgcc ctgcgctggc
    28381 gctaaatccc ggaatcgctc cctgccagtc aagattcagc ttcagccgct ccgggcaata
    28441 aataatattc tgcaaaacca gatcgttaac ggaagcgtag gagtgtttat cgtcagcatg
    28501 aatgtaaaag agatcgccac gggtaatgcg ataagggcga tcgttgagta catgcagacc
    28561 attaccgcgc cagacaatca ccagctcaca aaaatcatgt gtatgttcgg caaaaacatc
    28621 ttgcggataa cggtcagcca cagcgactgc ctgctggtcg ctggcaaaaa aatcatcttt
    28681 gagaagtttt aactgatgcg ccaccgtggc tacctcggcc agagaacgaa gttgattatt
    28741 cgcaatatgg cgtacaaata cgttgagaag attcgcgtta ttgcagaaaa ccatcccgtc
    28801 cctgacgaat atcacgcggt gaccagttaa actctcggcg aaaaagcgtc gaaaagtggt
    28861 tactgtcgct gaatccacag cgataggcga tatcagtaac gctggcctcg ctgtggcgta
    28921 gtagatgtcg ggctttcatc agtcgcaggc ggttcaggta acgctgaggc gtcagtcccg
    28981 tttgctgctt aagctgccga tgtagcgtac gcagcgaaag agaaaattgc tccgccacgg
    29041 catcccaatt cacctcatcg gcaaaatggt cctccagcca ggccagaagc aagttgagac
    29101 gtgatgcgct gttttccagg ttctcctgca aactgctttt acgcagcaag agcagtaatt
    29161 gcataaacaa gatctcgcga ctggcggtcg agggtaaatc attttcccct tcctgctgtt
    29221 ccatctgtgc aaccagctgt cgcacctgct gcaatacgct gtggttaacg cgccagtggg
    29281 aaggatactg cccatccagc tcttgtggca gcaactgatt cagcccggcg agaaactgaa
    29341 atcgatccgg cgagcgatac agcacattag tcagacacag attatcggta tgttcataca
    29401 gatgccgatc atggtcgcgc acaaaacaga ccgtgccacc ggtgatggta tagggctgcc
    29461 cattaaacac atgaataccc gtgccatgtt cgacaatcac aatttcatga aaatcatgat
    29521 gatgttcagg aaaatccgcc tgcgggagcc ggggttctat cgccacggac gcgttaccag
    29581 acggaaaaaa atccacacta tgtaatacgg tcatactggc ctcctgatgt cgtcaacacg
    29641 gcgaaatagt aatcacgagg tcaggttctt accttaaatt ttcgacggaa aaccacgtaa
    29701 aaaacgtcga tttttcaaga tacagcgtga attttcagga aatgcggtga gcatcacatc
    29761 accacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
    29821 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
    29881 gaaattcagc aggatcacat tatgaccttt cgcaattgtg tcgccgtcga tctcggcgca
    29941 tccagtgggc gcgtgatgct ggcgcgttac gagcgtgaat gccgcagcct gacgctgcgc
    30001 gaaatccatc gttttaacaa tgggctgcat agtcagaacg gctatgtcac ctgggatgtg
    30061 gatagcctgg aaagtgccat tcgccttgga ttaaacaagg tgtgcgagga agggattcgt
    30121 atcgatagca ttgggattga tacctggggc gtggactttg tgctgctcga ccaacagggt
    30181 cagcgtgtgg gcctgcccgt tgcttatcgc gatagccgca ccaatggcct gatggcgcag
    30241 gcacaacaac aactcggcaa acgcgatatt tatcaacgta gcggcatcca gtttctgccc
    30301 ttcaatacgc tttatcagtt acgggcgctg acggagcaac aacctgaact tattccacac
    30361 attgctcacg ctctgctaat gccggattat tttagttatc gccttaccgg caagatgaac
    30421 tgggagtaca ccaacgccac aaccacgcaa ctggtcaata tcaatagcga cgactgggac
    30481 gagtcgctac tggcgtggag cggtgccaac aaagcctggt ttggtcgccc gacgcatccg
    30541 ggtaatgtca taggtcactg gatttgcccg cagggtaatg agattccggt ggtcgccgtt
    30601 gccagccatg ataccgccag cgcggttatc gcctcgccgt taaacggttc acgcgccgct
    30661 tatctctctt ctggtacctg gtcattgatg ggcttcgaaa gccagacgcc atttaccaat
    30721 gacacggcgc tggcagccaa catcaccaat gaaggcgggg cggaaggtcg ctatcgggtg
    30781 ctgaaaaata ttatgggctt atggctgctt cagcgggtgc ttaaggagcg gaaaatcaac
    30841 gatctcccgg cgcttatcgt cgcgacacag tcacttccgg cctgccgctt catcatcaat
    30901 cccaatgacg atcgctttat taatcctgag gcgatgtgca gcgaaattca ggctgcgtgt
    30961 cgggaaacgg cgcaaccgat cccggaaagt gatgctgaac tggcgcgctg cattttcgac
    31021 agtctggcgc tgctgtatgc cgatgtgttg catgagctgg cgcagctacg cggtgaagat
    31081 ttctcgcaac tgcatattgt cggcggaggc tgtcagaaca cgctgctcaa ccagctatgt
    31141 gccgatgcct gcggtattcg ggtgatcgcc gggcctgttg aagcctcgac gctcggcaat
    31201 atcggcatcc agttaatgac gctggatgaa ctcaacaatg tggatgattt ccgtcaggtc
    31261 gtcagcacca ccgcgaatct gaccaccttt acccctaatc ctgacagtga aattgcgcac
    31321 tacgtggcgc ggattcactc tacacgaaag acaaaggagc tttgcgcatg accactcaac
    31381 tggaacaggc ctgggagctg gcgaaacagc gtttcgcggc ggtggggatt gatgtcgagg
    31441 aggcgctgcg ccaacttgat cgtttacccg tttcaatgca ctgctggcag ggcgatgatg
    31501 tttccggttt tgaaaacccg gaaggttcat tgaccggtgg gattcaggca actggtaatt
    31561 atccgggcaa agcgcgtaat gccagtgagc tacgtgccga tctggaacag gctatgcggc
    31621 tgattccggg gccgaaacgg cttaatttac atgccatcta tctggaatta gacacgccag
    31681 tctcgcgcga tcagatcaaa ccagagcact tcaaaaactg ggttgaatgg gcgaaagcca
    31741 atcagctcgg tctggatttt aacccctcct gtttctcgca tccgctaagc gccgatggct
    31801 ttacgctttc ccatgccgac gacagcattc gccagttctg gattgatcac tgcaaagcca
    31861 gccgtcgcgt ttcggcctat tttggcgagc aactcggcac accatcggtg atgaacatct
    31921 ggatcccgga tggtatgaaa gatatcaccg ttgaccgtct cgccccgcgt cagcgtctgc
    31981 tggcagcact ggatgaggtg atcagcgaga agctaaaccc tgcgcaccat atcgacgccg
    32041 ttgagagcaa attgtttggc attggcgcag agagctacac ggttggctcc aatgagtttt
    32101 acatggggta tgccaccagc cgccagactg cgctgtgcct ggacgccggg cacttccacc
    32161 cgactgaagt gatttccgac aagatttccg ccgccatgct gtatgtgccg cagttgctgc
    32221 tgcacgtcag ccgtccggtt cgctgggaca gcgatcacgt agtgctgctg gatgatgaaa
    32281 cccaggcaat tgccagtgag attgtgcgtc acgatctgtt taaccgggtg catatcggcc
    32341 ttgacttctt cgatgcctct atcaaccgca ttgccgcgtg ggtcattggt acacgcaata
    32401 tgaaaaaagc cctgctgcgt gcgttgctgg aacctaccgc tgagctgcgc aagctggaag
    32461 cggcgggcga ttacactgcg cgtctggcac tgctggaaga gcagaaatcg ttgccgtggc
    32521 aggcggtctg ggaaatgtat tgtcagcgtc acgatacccc agcaggtagc gaatggctgg
    32581 agagcgtgcg ggcttatgaa aaagaaattt tgagccagcg tgggtaaaca ctgccggatg
    32641 cggcgcgagc gccttatccg gcctacgggt cgaatgtcca tgcgggctgt caccctcacc
    32701 ccggccctct ccctgaaagg gcgagggaga aaaacatagc ctcgtgcggc aacggagctg
    32761 aacaccgctc cggacagtcc cctcgcccct acggggagag ggttagggtg aggggaacaa
    32821 gccgcaccat gtctaaacaa ggcaacctaa tttacaggaa cacagaacat gcaaaacatt
    32881 acccagtcct ggtttgtcca gggaatgatc aaagccacca ccgatgcctg gctgaaaggc
    32941 tgggacgagc gcaacggcgg caacctgacg ctgcgcctgg atgacgccga tatcgcgcct
    33001 tataaagaca acttccatgc acaaccgcgc tatatcccgc tcagccagcc catgccttta
    33061 ctggcaaata caccgtttat tgtcaccggc tccggcaaat tcttccgtaa cgtccagctt
    33121 gatcctgcag ctaacttagg cgtcgtaaaa gtcgacagcg acggcgcggg ctaccatatt
    33181 ctctgggggt taaccaacga agccgttcca acttctgaac ttccggctca ctttctctcc
    33241 cactgcgagc gcattaaagc caccaacggt aaagatcggg tgatcatgca ctgccacgcc
    33301 accaacctga tcgccctcac ctatgtactg gaaaacgaca ccgcggtctt tactcgccaa
    33361 ctgtgggaag gcagcaccga gtgtctggta gtgttcccgg atggcgttgg cattttgccg
    33421 tggatggtgc ccggcacgga cgaaatcggc caggcgaccg cacaagagat gcaaaaacat
    33481 tcgctggtgt tgtggccctt ccacggcgtc ttcggcagcg ggtcgacgtt ggatgaaaca
    33541 ttcggtttaa tcgacaccgc agaaaaatca gcacaagtat tagtgaaggt ttattcgatg
    33601 ggcggcatga aacagaccat cagccgtgaa gagttgatag cgctcggcaa gcgcttcggc
    33661 gttacaccac tagctagtgc gctggcgctg taaggagcaa acatgatccg caaagccttt
    33721 gtcatgcagg ttaaccccga cgcccacgaa gagtatcagc gtcggcataa ccccatctgg
    33781 ccggaactgg aagcagtgtt gaaatctcac ggtgcgcata actacgccat ttacctcgac
    33841 aaagcgcgta atctgctgtt tgccacggta gagattgaat ctgaagaacg gtggaatgcg
    33901 gtagccagca ctgatgtttg ccaacgttgg tggaaatata tgaccgatgt tatgcccgct
    33961 aacccggata acagcccggt gagtagcgag ctgcaagaag tgttttacct gccgtaattg
    34021 cccaatgtgc cagagatgcc tccggcacaa tattttaaat ttcgatttga aaaattaaac
    34081 atattacatc tcccggtaag ttatatttcc ctgatacatt gtgagtaaat cacaaaaata
    34141 atgaataacc cattaatgat tcatgtggtt tatttaaata accattatgt gcattactcc
    34201 gcaaatctga cctttcactc tgtctcaaat tgtcattgtt taccgacata tcgcctctca
    34261 tcacttaatc gctccaccat tacaattgat taaaatatat tttatggagt ccgattaatg
    34321 gcagctctta ctgcaagctg tattgacctg aatattcagg gcaatggcgc ttattccgtt
    34381 ctgaagcagt tggcgacaat agcgttacaa aacggtttta tcaccgactc acaccagttc
    34441 ctgcaaaccc tgctgctgcg cgaaaaaatg cactctactg gatttggttc cggtgtcgcc
    34501 gtgccgcacg gtaaaagcgc ctgcgttaaa caaccgttcg tattattcgc ccgcaaagcg
    34561 caggctattg actggaaagc cagcgatggc gaagacgtca attgctggat ctgcctcggc
    34621 gtgccgcaaa gcggcgaaga ggatcaggtc aaaatcatcg gcacactgtg tcgcaaaatt
    34681 attcacaagg aatttattca tcaactgcaa cagggcgata ccgaccaggt gcttgccttg
    34741 ttaaatcaaa ccctcagctc ataaggaagt ggcgatggag tcatccttac gtattgtcgc
    34801 gatcaccaac tgccccgccg ggatcgctca cacctacatg gtggcggaag ccctggaaca
    34861 gaaagcccgt tctctcggtc ataccattaa agttgaaact caaggatcaa gcggcgtcga
    34921 aaaccgctta tccagcgaag agattgccgc tgccgattac gtcattctcg ctaccgggcg
    34981 tggcctgagc ggtgacgatc gcgcgcggtt tgccgggaag aaggtttatg agattgccat
    35041 ctcccaggcg ctgaaaaata tcgatcagat tttcagtgaa ttaccaacaa actcgcatct
    35101 ttttgctgca gatagcggcg tgaagcttgg caaacaggaa gtgcaaagcg gcagcgtgat
    35161 gagccacctg atggcgggcg tctctgcggc gctgccgttt gtcatcggcg gcggtattct
    35221 ggtggcgctt gccaacatgc tggtgcagtt cggtttgcca tataccgata tgtcgaaagg
    35281 tgcgccgtcg tttacctggg tagttgaatc catcggttac ctcggcttca cctttatgat
    35341 ccccatcatg ggcgcttata ttgcctcgtc gattgccgat aaaccggctt tcgctccggc
    35401 gtttctggtt tgctatctgg cgaacgacaa agcgctgctc ggcacccagt cgggtgcggg
    35461 attcctcggc gcggtggtgc tggggctggc gattggctat ttcgtcttct ggttccgcaa
    35521 agtgcgcctt ggcaaagcat tacaaccgct gctcggttca atgctgatcc cgttcgttac
    35581 tctgctggtt ttcggcgtgc tcacttacta cgttatcggg ccggtgatgt ccgacctcat
    35641 gggcgggctg ctccacttcc tgaacaccat tcctccatca atgaagtttg cggcagcgtt
    35701 tctggtcggt gcgatgctgg cgttcgatat gggcggcccc atcaataaaa ccgcctggtt
    35761 cttctgcttc tcactgctgg aaaaacacat ttatgactgg tacgccatcg tcggcgttgt
    35821 cgcgttgatg ccgcccgtcg cggcgggtct tgcgacgttc attgcgccca aactgtttac
    35881 ccgacaggaa aaagaggctg ccagtagtgc cattgtggtc ggtgccacag tcgcaaccga
    35941 accggctatt ccttacgctc tggcagctcc gttgccgatg attaccgcca ataccctggc
    36001 gggcggcatt accggcgtgc tggtgatcgc tttcggaatc aaacgtctgg cgcctggttt
    36061 gggtatcttt gacccgctga tcggcctgat gtcgccggta ggttcgtttt atctggtgct
    36121 ggcgattggc ctcgcgctga acatttcatt cattatcgtc ctgaaaggat tgtggttacg
    36181 ccgcaaagcg aaagccgcgc aacaggagct tgtccatgaa cattgagtta ctgcaacagt
    36241 tgtgcgaagc cagcgccgtt agcggcgatg aacaggaagt tcgcaacatc ctgataaaca
    36301 cgctggaacc ttgcgttaat gagatcacct ttgatggtct gggcagcttt gttgcccgta
    36361 agggaaataa aggtccaaaa gttgccgttg ttgggcatat ggatgaagtc ggctttatgg
    36421 tcacccacat cgacgagagc ggttttctgc gcttcaccac cattggtggc tggtggaatc
    36481 agtcgatact caaccaccgg gtaaccatac gcacacacaa gggagtgaaa atccctggtg
    36541 tgattggttc cgtcgcgcct catgcgttaa cggaaaagca aaagcaacaa ccgctgtcat
    36601 ttgatgagat gttcattgat attggcgcga acagtcgcga agaggtggaa aaacgcggcg
    36661 tggaaattgg cgattttatt agcccggaag ccaattttgc ctgctggggc gaagataaag
    36721 tagtcggcaa ggcgctggat aaccgcatcg gctgcgcgat gatggcggag ctactacaga
    36781 cagtaaataa cccagaaatt acgctgtatg gcgttggcag tgtggaagaa gaagttgggc
    36841 tacgcgaggc acaaacctcg gcggaacaca ttaaaccgga tgtggtgatc gtgctggata
    36901 ccgctgtcgc aggtgatgtt ccgggcattg ataacattaa atacccgctg aaactgggcc
    36961 aggggccggg gctgatgctg tttgacaagc gctacttccc caaccagaaa ctggtagcag
    37021 cgtttaaaaa ctgtgccacg cagaatgatt taccgctgca attttccacc atgaaaaccg
    37081 gagcgacgga tggcgggcgc tacaacgtga tgggcggcgg gcgtccggtt gtcgcgctgt
    37141 gtctgccaac tcgttatctg cacgccaaca gcggtatgat ttcaaaagcc gattacgatg
    37201 ctctgctcac gctgatacgg ggttttctga ccaccttaac tgcggagaaa gtcaacgcgt
    37261 ttagccagtt ccgtcaggtg gattaatgct aaacgaacgc cagttaaaga ttgtcgatct
    37321 gctggagcaa cagccgcgca cgcctggtga gctggcgcaa cagactggcg tttcaggcag
    37381 gaccatcctg cgtgatattg actatctcaa ctttaccctt aacggcaaag cccgcatttc
    37441 cgccagtggt agtgcgggct atcagctgga aatcttcgag cgccgcagct tttttcagtt
    37501 gctgcaaaag cacgataacg acgatcggct gctggcactg ttattactga atacctttac
    37561 tccccgtgcg caactcgcct cggcgcttaa tttgccagaa acgtgggtcg cagagcgtct
    37621 gccccggtta aaacagcgtt atgaacgcgc ttgttgcctg gccagccgcc ccggtttggg
    37681 ccatttcatt gatgagacag aagagaagcg cgttatcttg ctggcgaact tgctgcgcaa
    37741 agatccgttt ttaattccgc tggcgggcgt aacacgagac aaccttcagc atttatccac
    37801 ggcctgcgac aaccaacacc gctggccgct catgcagggt gattatctct ccagcctgat
    37861 tctggcgatt tacgccctgc gtaatcaact gaccgatgag tggccgcaat atcccggtga
    37921 cgagataaaa caaatcgttg aacatagcgg tctgtttctt ggtgataacg ctgtaagaac
    37981 cctgacaggt ttgatagaga aacagcatca gcaagcgcag ataatttctg ccgataatgt
    38041 gcagcggttg ctgcaaaggg tgccgggcat cgcgtcattg aatattattg atacgcagct
    38101 ggttgagaac attaccgggc atttattacg ttgccttgcc gcaccggtgt ggattgctga
    38161 gcaccgccaa agcagcatga ataacctgaa agccgcctgg cctgcggcct ttgatatgag
    38221 tctgcacttt attacgctac tgcgtgaaca gctcgatatt ccccttttcg acagcgatct
    38281 gatcggtttg tattttgcct gtgcgctgga acggcatcaa aacgaacgcc agccgatcat
    38341 tttgctctcg gaccagaacg cgattgccac tattaatcag ctcgccattg agcgcgatgt
    38401 tttacattgt cgggtgatta ttgcccgtag cttaagcgaa cttgttgcca ttcgcgaaga
    38461 gattgagccg ttattgatca ttaacaacag ccattattta ctggatgacg cggtaaataa
    38521 ttacatcacc gtaaaaaata tcattacggc tgccggtatc gaacaaataa aacattttct
    38581 ggcgacggca tttattcgcc aacagccgga gcgttttttc tctgcccccg gaagttttca
    38641 ttattcgaat gtacgcggtg aaagctggca acatattacc cggcaaattt gtgcgcaatt
    38701 agtcgcacaa caccatatta ccgccgatga agcacaacgc atcatcgccc gcgaaggcga
    38761 aggtgaaaac ctgattgtta atcgcctcgc catcccacat tgctggagcg aacaggagcg
    38821 acgttttcgt ggatttttta ttaccctcgc ccaaccagtt gaggtgaata acgaagtcat
    38881 taaccatgtc ttgatcgcct gcgccgccgc cgatgcgcgt catgagctaa aaatatttag
    38941 ctatctggca agcatattgt gtcagcatcc ggcggagatt attgccgggt taacaggata
    39001 tgaggcattt atggagttac ttcacaaggg gtgaactgtg tcggatgcgc cgcatccgac
    39061 actgcattta tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg
    39121 tactcgcgta atgctctcgg gaaagagtct tcgaccatcc agcttgagtt tgcgtcctga
    39181 agttgctctt tatcccagtc agagtacggg actttatctc tgatgcggcg aacgtatttt
    39241 ttagcctcga gttgatactg gcctgccgaa ttaataaacg agaaattcat gccgccttta
    39301 tccgcttctt tggcctgcgc cactagcgtt tccagttcag acactttctt cattgccgca
    39361 tcaacatcaa acttctcttg cgatatcaga ttattaattt gtttcgctga aatcatgact
    39421 gccagagagt agtagtggaa tgttttccct tctctttctt caatattttt cagttccgca
    39481 agctggcgct tatcgttaat ttcctgaatc gccgcatgat aagattctgc tacagcttca
    39541 aaggcttcca gattttttaa gaatgtctgg tgcagcgttt tccctttcgc aaacgcatcg
    39601 tctttataat tttcctgggt gtaatattta tccatttcgt taatggtatt acccaacgcc
    39661 acggcagcat cgatataact caccgccacg ccatcaatag gttgcagcgc aggcgttaac
    39721 gccacagcac tttttacgcc tttttcacac tcagcgaggt tggattcact aatgccgtag
    39781 atgccataaa cagtacgctc ttcaccggta ggcccctgtt taaaatcttt cagccagtca
    39841 gcataacgcg ccagactgct ctgtacaggg atctgtaact tgttatagca cttgatatag
    39901 acatccattt tttcgtcgaa aaccgcttgt tcatcgacca ccggttgcgc aggtgtttca
    39961 ggtttagctt ctgcttttgc aacgggagct tcaggagctg gtgcggcagg ttgactattg
    40021 gcaggcgggg gcgtttctgt ttcggctttt ttatcatcac aacccgtcag accgagggcc
    40081 atgatggcga ctataatagc ggaagataat aaattcctt
//
LOCUS       DAZGXZ010000028        40008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000028 DAZGXZ010000000
VERSION     DAZGXZ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.028"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..278)
                     /gene="chbB"
                     /locus_tag="ACHKJF_001614"
     CDS             complement(<1..278)
                     /gene="chbB"
                     /locus_tag="ACHKJF_001614"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP0948993.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVL"
     gene            complement(577..915)
                     /gene="osmE"
                     /locus_tag="ACHKJF_001615"
     CDS             complement(577..915)
                     /gene="osmE"
                     /locus_tag="ACHKJF_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP0948994.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1117..1944
                     /gene="nadE"
                     /locus_tag="ACHKJF_001616"
     CDS             1117..1944
                     /gene="nadE"
                     /locus_tag="ACHKJF_001616"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP0948995.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2174..3061
                     /gene="cho"
                     /locus_tag="ACHKJF_001617"
     CDS             2174..3061
                     /gene="cho"
                     /locus_tag="ACHKJF_001617"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP0948996.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHIRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3021..3596)
                     /gene="ves"
                     /locus_tag="ACHKJF_001618"
     CDS             complement(3021..3596)
                     /gene="ves"
                     /locus_tag="ACHKJF_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP0948997.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3799..4284)
                     /gene="spy"
                     /locus_tag="ACHKJF_001619"
     CDS             complement(3799..4284)
                     /gene="spy"
                     /locus_tag="ACHKJF_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP0948998.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4614..5582)
                     /gene="astE"
                     /locus_tag="ACHKJF_001620"
     CDS             complement(4614..5582)
                     /gene="astE"
                     /locus_tag="ACHKJF_001620"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP0948999.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5575..6918)
                     /gene="astB"
                     /locus_tag="ACHKJF_001621"
     CDS             complement(5575..6918)
                     /gene="astB"
                     /locus_tag="ACHKJF_001621"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP0949000.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6915..8393)
                     /gene="astD"
                     /locus_tag="ACHKJF_001622"
     CDS             complement(6915..8393)
                     /gene="astD"
                     /locus_tag="ACHKJF_001622"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP0949001.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNIPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8390..9424)
                     /gene="astA"
                     /locus_tag="ACHKJF_001623"
     CDS             complement(8390..9424)
                     /gene="astA"
                     /locus_tag="ACHKJF_001623"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP0949002.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9421..10641)
                     /gene="astC"
                     /locus_tag="ACHKJF_001624"
     CDS             complement(9421..10641)
                     /gene="astC"
                     /locus_tag="ACHKJF_001624"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP0949003.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11088..11894
                     /gene="xthA"
                     /locus_tag="ACHKJF_001625"
     CDS             11088..11894
                     /gene="xthA"
                     /locus_tag="ACHKJF_001625"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP0949004.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12061..12771
                     /gene="ydjX"
                     /locus_tag="ACHKJF_001626"
     CDS             12061..12771
                     /gene="ydjX"
                     /locus_tag="ACHKJF_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0949005.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12776..13453
                     /gene="ydjY"
                     /locus_tag="ACHKJF_001627"
     CDS             12776..13453
                     /gene="ydjY"
                     /locus_tag="ACHKJF_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP0949006.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAGKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13468..14175
                     /gene="ydjZ"
                     /locus_tag="ACHKJF_001628"
     CDS             13468..14175
                     /gene="ydjZ"
                     /locus_tag="ACHKJF_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0949007.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDCMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            14175..14723
                     /gene="ynjA"
                     /locus_tag="ACHKJF_001629"
     CDS             14175..14723
                     /gene="ynjA"
                     /locus_tag="ACHKJF_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP0949008.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14733..15899
                     /gene="ynjB"
                     /locus_tag="ACHKJF_001630"
     CDS             14733..15899
                     /gene="ynjB"
                     /locus_tag="ACHKJF_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0949009.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPSTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15872..17407
                     /gene="ynjC"
                     /locus_tag="ACHKJF_001631"
     CDS             15872..17407
                     /gene="ynjC"
                     /locus_tag="ACHKJF_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP0949010.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGTGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17407..18060
                     /gene="ynjD"
                     /locus_tag="ACHKJF_001632"
     CDS             17407..18060
                     /gene="ynjD"
                     /locus_tag="ACHKJF_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0949011.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            18127..19434
                     /gene="ynjE"
                     /locus_tag="ACHKJF_001633"
     CDS             18127..19434
                     /gene="ynjE"
                     /locus_tag="ACHKJF_001633"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP0949012.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRLSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19443..20063)
                     /gene="ynjF"
                     /locus_tag="ACHKJF_001634"
     CDS             complement(19443..20063)
                     /gene="ynjF"
                     /locus_tag="ACHKJF_001634"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0949013.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            20150..20557
                     /gene="nudG"
                     /locus_tag="ACHKJF_001635"
     CDS             20150..20557
                     /gene="nudG"
                     /locus_tag="ACHKJF_001635"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP0949014.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20523..20795)
                     /gene="ynjH"
                     /locus_tag="ACHKJF_001636"
     CDS             complement(20523..20795)
                     /gene="ynjH"
                     /locus_tag="ACHKJF_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP0949015.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21031..22374
                     /gene="gdhA"
                     /locus_tag="ACHKJF_001637"
     CDS             21031..22374
                     /gene="gdhA"
                     /locus_tag="ACHKJF_001637"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP0949016.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22491..23531)
                     /gene="ynjI"
                     /locus_tag="ACHKJF_001638"
     CDS             complement(22491..23531)
                     /gene="ynjI"
                     /locus_tag="ACHKJF_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0949017.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23659..25620)
                     /gene="topB"
                     /locus_tag="ACHKJF_001639"
     CDS             complement(23659..25620)
                     /gene="topB"
                     /locus_tag="ACHKJF_001639"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0949018.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVIERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(25625..26668)
                     /gene="selD"
                     /locus_tag="ACHKJF_001640"
     CDS             complement(25625..26668)
                     /gene="selD"
                     /locus_tag="ACHKJF_001640"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP0949019.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(26785..27336)
                     /gene="ydjA"
                     /locus_tag="ACHKJF_001641"
     CDS             complement(26785..27336)
                     /gene="ydjA"
                     /locus_tag="ACHKJF_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP0949020.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27497..29353
                     /gene="sppA"
                     /locus_tag="ACHKJF_001642"
     CDS             27497..29353
                     /gene="sppA"
                     /locus_tag="ACHKJF_001642"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP0949021.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPAVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29520..30536
                     /gene="ansA"
                     /locus_tag="ACHKJF_001643"
     CDS             29520..30536
                     /gene="ansA"
                     /locus_tag="ACHKJF_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP0949022.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30547..31188
                     /gene="pncA"
                     /locus_tag="ACHKJF_001644"
     CDS             30547..31188
                     /gene="pncA"
                     /locus_tag="ACHKJF_001644"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP0949023.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWVRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31349..31621)
                     /gene="yeaC"
                     /locus_tag="ACHKJF_001645"
     CDS             complement(31349..31621)
                     /gene="yeaC"
                     /locus_tag="ACHKJF_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP0949024.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(31662..32075)
                     /gene="msrB"
                     /locus_tag="ACHKJF_001646"
     CDS             complement(31662..32075)
                     /gene="msrB"
                     /locus_tag="ACHKJF_001646"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP0949025.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            32417..33412
                     /gene="gapA"
                     /locus_tag="ACHKJF_001647"
     CDS             32417..33412
                     /gene="gapA"
                     /locus_tag="ACHKJF_001647"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0949026.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            33496..34380
                     /gene="yeaD"
                     /locus_tag="ACHKJF_001648"
     CDS             33496..34380
                     /gene="yeaD"
                     /locus_tag="ACHKJF_001648"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP0949027.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(34431..35285)
                     /gene="yeaE"
                     /locus_tag="ACHKJF_001649"
     CDS             complement(34431..35285)
                     /gene="yeaE"
                     /locus_tag="ACHKJF_001649"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP0949028.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(35375..36121)
                     /gene="mipA"
                     /locus_tag="ACHKJF_001650"
     CDS             complement(35375..36121)
                     /gene="mipA"
                     /locus_tag="ACHKJF_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP0949029.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            36557..38491
                     /gene="yeaG"
                     /locus_tag="ACHKJF_001651"
     CDS             36557..38491
                     /gene="yeaG"
                     /locus_tag="ACHKJF_001651"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP0949030.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            38604..39887
                     /gene="yeaH"
                     /locus_tag="ACHKJF_001652"
     CDS             38604..39887
                     /gene="yeaH"
                     /locus_tag="ACHKJF_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP0949031.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         9693 a        10485 c        10049 g         9781 t
ORIGIN      
        1 agcacgccta aaccatcgac tttgccataa agcagcgagt caattacttc aaccggtttg
       61 ttgggtaaca aacgctggat ttcgggcaac atataagcaa tctgcggccc taataacacg
      121 acatcggcat tctgaccttt ttcaccagcc agtgtttccg gaaatgcttc aataatgacc
      181 ggaacttcat atttttctgc ctgtgcgcgc atttttgata ccagtaaaga ggtagacatg
      241 cccgcagaac aaaacagata aatgtgtttc ttttccataa aactgccctc atcgacgatt
      301 atctgtcagc cagacactcc gcaagcctta acctgcttcc atgctctggg taacttgcga
      361 aaccaacatg actttttttg tggctgaaag gagtatacgg tatagacctg agcaatagta
      421 tttctttgac cttataaatt gtctctcctt gacctgcggt tatgaccctc ttcacatttc
      481 gggcaaataa ttcgcgaaga taattaagaa ctcaggccaa aaaaaaccgg cacaatggcc
      541 ggtttccgtt gttactcaag ctttcagacg aattgattac ttcgcagcct gtggatcagt
      601 gtcgtattca gcacaggtct gataaccgga gttgatgaca tgtccggtat catctaacgc
      661 gacaaagtag gtttctgctt taccatcacg ttgacccagg atgtaggtct ggcaagtacc
      721 acgagcatgg atcatgctca cttcagacga aggtttaccc gcaatttgtg caacctgcgc
      781 ccggctcatg ccttttttga cgtctttcac cacaggctgt acaaactggt ctttagtacg
      841 atcataagcc gtacaacccg ccagcatggt taataccgcc gctgcactca gaattcctgc
      901 catattcttg ttcatattcc gtcctcttgt ttatcagcgt gttagataag cctggaatac
      961 attgggcgct ttttcaagcc cgtgaacgaa acggctccgc tttcagagga ttcctgtatg
     1021 acgttttaac caccattcag cccgctgtcg cttgtcgttt cagtggcaac gggttagctt
     1081 taaggaagtt ttgtcttttc tgtctggagg ggttcaatga cattgcaaca acaaataata
     1141 aaggcgctgg gcgcaaaacc gcagattaat gctgaagagg aaattcgtcg tagtatcgat
     1201 tttctgaaaa gctacctgca aacttatccg ttcattaaat cactggtgct cgggatcagc
     1261 ggcggtcagg actccacgct tgccggaaag ctgtgccaga tggcgattaa tgagctgcgc
     1321 ctggaaaccg gcaacgaatc actgcaattt attgccgtac gcctgcccta tggtgttcag
     1381 gccgacgaac aagattgcca ggatgccatt gcctttattc aaccggatcg cgtattaacc
     1441 gttaatatca agggcgcggt attggccagc gaacaggcat tgcgggaagc aggcattgaa
     1501 ctgagcgatt ttgtccgtgg caatgaaaaa gcgcgtgagc ggatgaaagc acaatatagc
     1561 attgcgggta tgaccagcgg tgtcgtggtg ggcaccgatc atgcagcaga agccattacc
     1621 ggattcttca ctaaatatgg tgacggcggt acggatatta atccgctgta tcgtctcaac
     1681 aaacgccagg gtaaacagtt gctgacggca ttaggttgcc cggaacacct ttataagaaa
     1741 gccccaacgg ccgatctgga agatgatcgc ccttctctgc cagatgaagt ggcactcggc
     1801 gtgacctatg acaatatcga cgactatctg gaagggaaaa acgtacctga acaggtcgcc
     1861 agaacaatag agaactggta tctgaaaacc gaacataaac gccgtccgcc aattaccgtt
     1921 ttcgatgatt tctggaaaaa gtaataattt gcacataatg gtttgtgcga aaaagagtaa
     1981 tttgttcacg ccggatgcgg cgtgaacgcc ttattcgacc tacaaaacta tgcaaattca
     2041 atatattgca ggagtcgaat aggcctgaca ggcgtagcac ttcagacggt gtaacctttg
     2101 tcatcgaccc gcctcttttt taatcgcttc ccgcctgtta cactggatag ataaccagca
     2161 ttcggagtca acagtggtac ggcgtttaac ttctccgcgg ctcgaatttg aagctgcggc
     2221 aatttatgaa tatcccgaac atttacgttc attccttaat gacttaccca cccgaccagg
     2281 ggtgtatctg tttcatggcg aaagtgacac catgccgctc tatatcggca aaagcgttaa
     2341 tatccgcagt cgggtgcttt ctcatatacg caccccggat gaagccgcca tgctacggca
     2401 atcccggcga attagctgga tctgcaccgc aggcgaaatc ggcgctctgc tccttgaagc
     2461 gcgattaatc aaagaacaac agccgctgtt taataaacgg ttgcgccgca atcgccagct
     2521 ctgtgccctg caattaaatg aaaagcgcgt cgatgtggtg tatgccaaag aggtggattt
     2581 ttcacgagcc cccaacctgt ttggcctgtt tgccaatagg cgcgcagctt tgcaagcatt
     2641 gcagagcatc gctgatgaac aaaaactttg ttatggcctg ctgggactgg aaccgttaag
     2701 tcgcggtcgt gcatgttttc gttcagcgct aaaacgttgc gccggagcat gctgcggtaa
     2761 agagagccat gaggaacatg cgctacgctt gcgccagtct ctggagcgtt tgcgggtggt
     2821 gtgttggcct tggcaagggg cggtggcact gaaagaacag cacccggaaa tgactcaata
     2881 tcatattatt caaaactggc tgtggctggg ggcggttaat tcgctggaag aagcgacaac
     2941 gttaattcgg acacccgccg ggtttgatca cgacggttat aaaattcttt gtaagccgct
     3001 gctttccggt aactatgaaa ttactgaact tgatccggcg aatgaccagc gagccagttg
     3061 atttcactga acaacagttt accttgcggt tgtaaaagcc gcagggtgtg cctgccatcg
     3121 aaccagcacg cgccctggtc ggtagtgagc aatttatcgc ccagttgcca cgccccgtta
     3181 atcacaaaca ccaccccgcc gcgtgagcca aaggtagtaa acgtacgttc ggcaattctg
     3241 actttcgcct tacagacatc caggcgtgtc atgatgttga aatccatcga catctgccct
     3301 gccgtcagtt tcgcttttac cacctggtcc gctgcaaagg caaaaggctg taacggtttt
     3361 aaggtatggt taaagcggtc tgcgctttca aggagcatct cgccgccttc cagcaacgtc
     3421 actatccttt ccatgccggg aaacaaagaa aactccccat tcgccgcgat ggatgcaatg
     3481 ctggcacgcc agtaaaaatc acgttttgcc ggtgggaaag tgcaaatttc gcgcgtttca
     3541 ccggcagcat ttcgccacag attcaccgac attttacgca tatcaaagta ttccatactc
     3601 gctcccggcc cgttcttttc attgttatcg ttcaggtaac cgtggatggt tatcggcaac
     3661 tcgggtgatt tacgttagtg gtgatcaggc tttttttaca actgtttgag aagagaaaag
     3721 aaaaccgccg atcctgtcca ccgcattact gcaaggtagt ggacaagacc ggcggtctta
     3781 agttttttgg ctgaaagatt attcagcagt tgcaggcatt ttaccttttg ccgctggacg
     3841 ttctgtcaga cgcttctcaa aattagcatt aaattgcttt ttctgttccg gcgtcaggat
     3901 gttgtaaatt ttgttctggg tttccatgtg cgccagcatg ttagctttgc gctgttcttc
     3961 catttttgcg atctgcgctt cagcttttgc tttatcgaag gtatcgctgg caatgatgtc
     4021 atgcattgcg cggcgttctt ccagcggcgg acgtttcatc tggtcacgct ggcctttcat
     4081 gatttcgcgg atctgctgtt tctgcgcgtc ggtcaggttc aggtctttga acatcatgtc
     4141 ctgatgcgga ccgaacttgc ctttgtggtg catcatcggc ttcgcgtcag ccggtgctgc
     4201 ggtagtggtg tctgcggcat gggccaggtt agccgcgcca agagccaggg tagaggcaac
     4261 aaacagtgca gttaatttac gcatattcta tatccttcct ttcagttatt tattacggct
     4321 ttcttaagta gcgtgccgtg ttgacgagat taactttact gactttagcg tcaattaatc
     4381 agagcaacgg taaaacaatg aaagtgtaaa aaacactttt gcgccaatta tggagaaaaa
     4441 aagaaaattt gatggagagt gatgagagaa tattacaaca cgatgatttt gcagagatta
     4501 tgaagaacta taccggatga ctggtgataa ataaagcaaa taaccaggat taatctgtat
     4561 taatttataa gaaggcaact taatacccgc agaatgattt ctgcgggtaa gtattagctt
     4621 attttttcga gcattaatcc cgcgcgtaat cccaacgcta ccaacggatt agggaataac
     4681 acatactcta catcatgggt tacggtaaaa cgttcctctc cgtcctgcgc cagcaatgtt
     4741 cctttctcaa acggcataaa attcagcgtg tcacttgcca tatgcatttc gaaggacggc
     4801 gagtggcgag taatttgcga aaccacccga taacggagcg gcggtgttct cacgataccg
     4861 acactctcac cagatagcag cgcagcaatt gcgctggcag ttactgcaaa ctggcgaaga
     4921 tcgttttgcc caaagggcaa cgctttgcca agttccagcg tacaggccag cgcgccaaaa
     4981 tgtctggcgc tgaaatgggt aaacgtacca ccaggttcct gatggaacac cagcgcctcc
     5041 agccccgccg cacccagcca cgtcagaaat ttctcgtccc agggaatgtc gcgttgcggt
     5101 aatacaccga actgcggatg caaggagcca cgaattgcgg tatgtagatc aaggtgccag
     5161 cgcacagatt ctttgcccag gtcataaaaa tcttccaggc actgttccag ttcgcgcgcc
     5221 cgacaggttt ctccgctttc agcaaatagc tgccaacgac cgccaaacat tcgattcata
     5281 tcgctatggc aataacgttt cccttgcttc agcgcaggag gattcccgag gatcaccagc
     5341 aaccgccaac gtaacgggat ctcgccgtga gatatcgcgc caagcaacgc gtccagcatc
     5401 tccacaggtg ccgtctcatt accgtgtatt cccgctgaaa tcaccagtgc gccttgcggt
     5461 ggcgttaatg gcgtcagttc cagcacacca tcgcccagcc agcgccagcg aacgccgttg
     5521 atttctcgct cggtgataac cggtttttta cccgttaagg tcagagcaag aaaattatcc
     5581 attgccccct ccctcgcgct ggaacggata aaccgaaccg agattcagta attggctcaa
     5641 tacatccagt gcttcccgcc ctccgcgcag caattgcggg tcggccagat cggcagcagt
     5701 aaggcgatcg cggtagtaac gatccaccca gtcattgagc gcattaaaca gcgtatcgtt
     5761 catcatcacc gccggattca ccgcccggcg ttcttcttct gtcaatacca cccgcaaccg
     5821 caggcacgcc gggccgccgc cattcgccat gctttcacgt aaatcaaaga cttttagttc
     5881 gctaatcggg ttgtcagcgg caaggagttc attgagataa ccccatactc cggcgtgttc
     5941 ccgacactcc tgaggcagca ccaacatcat ggaaccatca tcgcggctca acagttggct
     6001 gttaaacaga taggtagaca ccgcatccga cacggaaacc tgagttgccg gaacttctat
     6061 cgccataaaa ccgttcaccc gcgcacgcag gtttgccagt aactgtgact ggcgagcgaa
     6121 cgcctgttgg tggcaaaaca gcacctggcg gttactcacg gcaatcacgt cattatgaaa
     6181 aacgccctgg tcgataacgt ccgggttttg ctgggcgaaa atcacctgtt ggggattcac
     6241 ctgattcagc cttgccaccg cctcgctggc ttcgcgagtc tgtcgcgccg gataacggga
     6301 aggccgggta tcattgcctt cttctcgccc gtagacaaaa agttgcatac ccggctcacc
     6361 gtaatgaccg ccgagacgat tgtggtttgc cgccccctca tcaccgagca acgctacctg
     6421 cggcaacgcc gaatgaacgc taaatttctc ttcgtcgtta aaaatcgctt ttaaaagtga
     6481 ttcggtgacg tgcgcttcca gcgagcggtg aaatttattg ttcaggttgg caacggtgag
     6541 atgcactttg ccatccagcg tatcggcaga tggcgcgatc gttgccgcat tggctaccca
     6601 cattggcgaa gcggaactga cgctggaaag ccagtgcggt gcctggcgtg caactttttc
     6661 cagtacctgc tcatcgctac cactgaatcc caactgacgc agcaccggaa taaacggacg
     6721 ctcgtgcggc gggatcacgg cctgggggaa tcccgcatcg gcaagggctt tcattttcag
     6781 taagccctgt tttgccgcct gtcgcgggtt agacacctga aaacggtgac gggtagaggc
     6841 ttcattacca aacgacaggc cagcgtaatg atgcgtcagc cctaccagcc cgtcgaaatt
     6901 aacttcccag gcgttcatcg caccacctca tcggaaaaat ccagcccggg gttaagcgtt
     6961 gcgggcaaag ttaacgagtc cgactccagg ctcgccatag gccatgcgca gtaatctgcg
     7021 gcataccagg cgctggggcg atggttaccg gatgcaccaa tgccgccgaa tggcgcggta
     7081 ctggcagcac cagtaagcgg tttgttccag ttaacaatcc ccgcccgcgc ctccagcaac
     7141 agttgatcga atttttcccg ctcgggggaa accagaccgc aagagagtcc gaagcgagtg
     7201 ttattcgcca ttagaatcgc ttcctcgaaa gaatcataac gccagacgcg caataacggt
     7261 ccgaacacct cttcatctgg tacgccagca acgcctgtca tttcaatgat ccccggcgtc
     7321 agcaacgatg tctctgattg taataagcgc ggcgcaagca gggttcgtcc gcccatcgct
     7381 tccagttgct gccaggcagt aaccacctgc tgtgcggcct gttcagaaat cagcccgcca
     7441 ataaacggct gcggttcgtc atcccagttg cccggcgtta atcgctggct gacggcaacc
     7501 agacgagcaa gaaacgcatc gccctgcgct ccgcttttga gcaataaacg gcgggcgcag
     7561 gtgcagcgtt gcccggctgt gacaaacgcc gactgaatgg tcagatggac agccgcgtcg
     7621 atatccgcca cctcatcgat aattagcgga ttattaccgc ccatctcaag ggcgagaatt
     7681 ttctccggct gaccggagag ctggcgatgc agctggtagc cggtattggc gctaccggta
     7741 aacagcaaac cgtcgagatc ctccagcgca ctcagcgcct gacccgtttc acgcccgccc
     7801 tgcaccaggt taagtacgcc cggcggcaag ccagcctgct gccataaacg cattaccgct
     7861 tcgccactcc acggtgtcag ttcgctgggt ttaaagataa tggtgttacc tgccagcaat
     7921 gccggaacga tatgtccgtt cggcaaatga ccagggatat tatacggccc aaacaccgcc
     7981 agcacgccgt gcgggcgatg tcgcaggctc gccgcaccgt ccggcatttc actacgctgc
     8041 tcgccggtac gaacgtgata cgccttaatt gatatcgcga ttttattgat catcgccgtc
     8101 acttcggttg ccgcttccca gcgaggctta cccgtttctc tggcaataat cgcggttaat
     8161 tcgcctttat tcctttccag caatccggca aagcgttcga caacgacctg acgttcagcc
     8221 aatgagagcc gcgcccagcg cggaaacgcc gcacgggctg cgcgacaagc ctgcccgacc
     8281 tgagcggcat cggcatcatt gccttgccat aacacctcgc ccgataccgg attacgcttc
     8341 acacgcgatg cgccctggcc cgttatccag tcaccgttaa tccataaagt catgctgttt
     8401 tctcctctgc gcacaggcgc accagacgaa cgcgatcccc tgcgtggcat ttgagggcat
     8461 ccagttgtgc ggcggttaaa atcaaacgct cggttgccgg atcggtacgc gccagcacca
     8521 cgcggaaatg gtgataattt tcattggcga ccaggcaggc cggaaaatcg ccctgcgcag
     8581 gctgcccttc tgccacttcc accagccgac ttttacgaat ggcgcgcacg cggtcgatgt
     8641 cacactcaag cgttggcccg ccgtcgaaga tgtcgatata gttgcggtaa cgaaaacctt
     8701 ctttctccag caccgcgcgg gcaggcgcgg tttgcggatg tacctgaccg atgacgtcct
     8761 gggcttcctg ggataaaaag tgggtataga tcggatgttt cggcatcagt tctgcaataa
     8821 atgccttttg cccggtgccg cagagaaaat cggcgcggct aaaatccatc gaaaagaagc
     8881 gtttaccgag gctttgccag aacggtgaat agccgtgttc gtcaatcacc ccgcgcattt
     8941 cagcaaccac tttgtcatta aacttgtcgc gaaaagccgc cataaacata aagcgcgatt
     9001 tcgacagcaa atagccgttg ccctctttgc gccagtccgg gtcgagaaac agcgtgcaca
     9061 gctcgctgct gccggtgtga tcgttactga gaaacagcgt cggcaatgcg ttatagacat
     9121 tcagctcttt tgaggcgtga accaacgtgc cgacgcgata gttgtaccag ggatcgttca
     9181 gcccaaccgc cacctcaatg gcacaaatcc ccgccacggt gcctgtctcg ctatcttcca
     9241 gcacgaacac atagccctgc tcacttttgg gcagttcgcc ttgccaggtt ttgattgccc
     9301 tttcgatacg cgccgaaagc gtggcttcat tggcgggaag cgacgtcagg ccgccgcccg
     9361 ttttgctggc aagctgcatc agcgccgaga catctgatcg ctcaacggga cggatgacca
     9421 tcatgatgaa cctcggctaa caaagtgttc gcaagcagct gcaaagcgat ccagtccggt
     9481 cgtcacctct tcttcgctga cattgagcgc aggcgcaaaa cgcaccacgt tgccacccgc
     9541 aatcagtacc atcacgcctg ctttcgccgc ttcctgagag atctgtttcg cttgcccggc
     9601 gtaatcggca ttcagtacac agccaatcag caaacctaag ccgcgaactt cactgaacaa
     9661 accatagcgg tgattaatag tattaagacg ctcaacaaac cagtcgtgac gctgtttaac
     9721 gccattaagc atctctggtg tgttgatgag ctccagcact ttgcctgcca ccgccgaggc
     9781 cagcggatta ccgccatagg tggtgccatg agtgccaacg gtcatcacgc gggcgcactc
     9841 ttcggttgcc aacaacgcac cgaccgggaa accgccgccc agcgctttgg cggtagttaa
     9901 cagatcaggc gtcacgccgt agtgcatata ggcatacagt tccccggtgc gcccgacgcc
     9961 ggtttgtact tcatcaaaaa tcaacagcgc attgtggcgg ttacacaatt cacgcagacc
    10021 ttgtaaaaac gcgttgctgg ctggcaccac accgccttcc ccctggatgg gttcgacaat
    10081 caccgcacag gtagagtcgt caatcagcgc gctggcagag ttaatatcgt tatatgcagc
    10141 atgacgaata tccgccggca gtggcgcaaa atcctgtgaa taggctggct gcccacccgc
    10201 actgacagta aacagcgtgc gaccatgaaa cgcatttttg aacgccacga tgccgctctt
    10261 atggctgccg tagcggtcgt gagcgaattt acgcgccagt tttagcgccg cttcgttggc
    10321 ttccgcaccg gagttacaaa agaagacgcg atcggcaaac gtggcgtcga tcaatttttt
    10381 cgccagtcgc agtaccggct cgttggtgta accgttgccg gtatgccaga acttactcgc
    10441 ctgttcgttc agcgcttcac gcagttccgg atgcgcatgg cccagcgcgt tcaccgcaat
    10501 gccacccgcg aagtcgatat actctttccc ctgctgatcc cacaagcgcg aaccttcgcc
    10561 acgtaccggt ataaagggtg ccggagcgta aacaggtatc atccattcat caaagttttc
    10621 acgcgtaatt ggctgagaca tagcgacctc tacagtaaat aattcgttat ttatatgtta
    10681 acaataagta atgtttgcgc tgtaaatgta gattgcaggg ttcgtgccag ccagttgata
    10741 aaagtgcata aacggcggag gctaactgga aatcaaggag ttataaccaa accatatgca
    10801 tttaaagtgc atataaagtg aatacgtttg cgatgtgggt gaataaaaag aataaaaaac
    10861 gcaatgttat gcagaagtaa aatataattc tggaattgtg atcattgacg aaatttactg
    10921 gaaattactg cgccattctg acgcagcgcg caccaaaagc gggcattttt tgcgccatcg
    10981 ttgacatcat taacaaccat cgatcaaatc acttaacaac aggcggtaag caacgcgaaa
    11041 ttctgctacc atccacgcac tctttatctg aataaatggc agcgactatg aaatttgtct
    11101 cttttaatat caacggcctg cgcgccagac ctcaccagct tgaagccatc gtcgaaaagc
    11161 accaaccgga tgtgattggc ctgcaggaga caaaagttca tgacgatatg tttccgctcg
    11221 aagaggtggc gaagctcggc tacaacgtgt tttatcacgg gcagaaaggc cattatggcg
    11281 tggcgctgct gaccaaagag acgccgattg ccgtgcgtcg cggctttccc ggtgacgacg
    11341 aagaggcgca gcggcggatt attatggcgg aaatcccctc actgctgggt aatgtcaccg
    11401 tgatcaacgg ttacttcccg cagggtgaaa gccgcgacca tccgataaaa ttcccggcaa
    11461 aagcgcagtt ttatcagaat ctgcaaaact acctggaaac cgaactcaaa cgtgataatc
    11521 cggtactgat tatgggcgat atgaatatca gccctacaga tctggatatc ggcattggcg
    11581 aagaaaaccg taagcgctgg ctgcgtaccg gtaaatgctc tttcctgccg gaagagcgcg
    11641 aatggatgga caggctgatg agctgggggt tggtcgatac cttccgccat gctaatccgc
    11701 aaacagcaga tcgtttctca tggtttgatt accgctcaaa aggttttgac gataaccgtg
    11761 gtctgcgcat cgacctgctg ctcgccagcc aaccgctggc agaatgttgc gtagaaaccg
    11821 gcatcgacta tgaaattcgc agcatggaaa aaccgtccga tcacgccccc gtctgggcga
    11881 ccttccgccg ctaatttagc agctctcctg gctcaaactg ggtcaggaga attaaccttg
    11941 agaaaaatca acaaactgtc agtaatgatt tgttgcctgc cgtcctttgt tataccgtca
    12001 ctgcgttttt agttgtctga ccacttctct attatcaagt ttgatatagg aaactccacg
    12061 atgaacgctg agcgtaactt tctgtttgcc tgtcttattt ttgcgctggt catttacgct
    12121 atccacgctt tcggtttatt cgatctgctc accgatttac cccacttaca gacactcatt
    12181 cgccagagcg gattgttcgg ttatagcctc tatattctgt tattcatcat tgccaccctc
    12241 tttctgttac caggaagcat actggtgatc gctggaggaa tagtttttgg cccgctctta
    12301 gggacactac tctcattaat tgccgcaacg ctggcctcct cgagctcatt cctgctggcg
    12361 cgctggctgg gacgtgattt actgctgaaa tacgtgggcc atagccatac cttccaggcc
    12421 attgaaaaag gcattgcgcg taacggtatt gattttctta ttctgacccg cttaatcccg
    12481 ttgtttcctt acaatattca aaattacgct tacggattaa ccaccatcgc cttctggcct
    12541 tataccctta tttcggcact cacgaccctg cccggtattg ttatttatac cgtgatggca
    12601 agcgatctcg ccaatgaagg cattacgctg cgctttattt tacaactctg tctggcgggc
    12661 ctggcgctgt ttattctcgt ccagctcgca aaactctacg cccgacacaa acatgtggat
    12721 ctgtctgctt cgcgccgcag cccacttact cacccaaaaa atgaaggata gaacgatgtc
    12781 gcaacattat tcagtgtcat ggaaaaaagg actggctgca ctctgcttac tggctgttgc
    12841 cgggcttagc ggctgcgatc aaaaagagaa tgcggcggga aaagtggaat acgacggact
    12901 ttcgaacagc caaccgctgc gcgtcgatgc caataaccat acggtaacca tgctggtgca
    12961 aattaatggt cgtttcctca ccgacgacac tcgtcacggt attgtgttta aagatggctc
    13021 caacggacat aaatcgctgt ttatggctta tgcgaccccg aaagcatttt atgaagccct
    13081 gaaagaggca ggtggtacgc cgggcgaaaa tatgacgatg gataataaag aaacgactct
    13141 tgtcacaggc agcaaactgg atatttcggt caactggcaa ggggcggcaa aagcgtattc
    13201 cttcgatgaa gtgattgttg acagtaatgg caagaaactg gacatgcgct ttggcggtaa
    13261 tttaacggca gcagaagaga agaaaacggg ttgcctggtg tgtctggata gctgcccggt
    13321 cggcatcgtc agcaatgcaa catacactta tggtgcggtg gaaaaacgcg gcgaagtgaa
    13381 attcaaaggc aatgcctcag ttctcccggc ggataacacg ctggcaacgg ttacctttaa
    13441 aatcaccgaa taaagccagg ataaaggatg atgaagatac aatcgcgtaa aatctggtac
    13501 taccgtataa ccctcatcat cctgttgttc gctatgctgc tcgcatgggc gctgcttccc
    13561 ggcgtccatg agtttatcaa tcgcagcgtt gcggcgtttg ccgccgtgga ccaacagggt
    13621 atagaacgct ttattcagtc ttacggcgca ctggcggcag ttgtctcgtt cttgttgatg
    13681 attttacagg ccattgctgc accgctacct gcgtttttga tcacctttgc caatgcgtcg
    13741 ctgtttggcg cgttctgggg cggcttgctg tcgtggacca gttcgatggc cggcgcggcg
    13801 ctgtgctttt ttatcgccag agtgatgggc cgcgaagtgg tggaaaaatt aaccggcaaa
    13861 accgtgcttg actgtatgga cggctttttc actcgctacg gcaaacacac catcctggtc
    13921 tgtcggttat tgccttttgt tcctttcgat ccaatcagct atgctgccgg tctgacttca
    13981 atacgttttc gctcgttttt tatcgccacc gggcttggtc aattaccggc gactattgtt
    14041 tattcctggg cgggcagcat gttaacaggc ggtactttct ggtttgtcac cggactgttt
    14101 attctgtttg ccctgaccgt ggtgattttt atggcgaaga aaatatggct tgagcgccag
    14161 aagaggaatg actgatgggt ttaccgccgc ttagcaaaat tcctttcatt ttacgtccgc
    14221 aggcgtggct gcatcgtcgc cattacggcg aggtgctaag cccgattcgc tggtgggggc
    14281 ggatcccgtt tatcttttat ttggtgtcaa tgtttgttgg gtggctggag cgcaaacgct
    14341 caccgctcga tccggtagtg cgatcgcttg tcagcgcgcg cattgcgcaa atgtgcctgt
    14401 gtgagttttg cgtggatatc accagtatga aagtcgccga gcgtaccggc agcaccgata
    14461 aactgctggc agtggctgac tggcggcaaa gcccgctctt tagcgatgaa gaacggctgg
    14521 cgctggagta cgccgaagcc gcaagcgtca caccgccaac agtcgatgat gccctgcgta
    14581 cccgactggc tgcgcatttt gacgctcagg cgctcaccga actgacggca ttgatcggcc
    14641 tgcaaaacct gtcggctcgt tttaattctg ccatggacat tcccgctcag gggctgtgcc
    14701 gtattcctga aaaacgttct taaggagaga tgatgcgcca ttgtgggtgg ttgctgggat
    14761 tgttatcgct gttttctttg gcaacacatg ccagtgactg gcaggaaatt aaaaatgagg
    14821 ccaaagggca aaccgtctgg tttaacgcct ggggcggcga taccgcaatt aaccgctatc
    14881 tcgactgggt tagcggcgag atgaaaaccc attacgctat aaacctgaag attgttcgtc
    14941 tcgcggatgc cgcagacgcg gtgaagcgca ttcagaccga agctgctgcc ggacgtaaaa
    15001 cgggcggctc ggtggatctg ctctgggtga acggcgaaaa cttccgcacc ttaaaagagg
    15061 ccaatttatt acaaacgggc tgggcggaga ctctgcccaa ctggcgctat gtcgacacac
    15121 agctgccggt gcgggaagat ttttcagtgc cgacacaagg tgcggaatcg ccctggggcg
    15181 gcgcacaact gacgtttatc gcccgccgcg atgttacgcc acagccacca caaacgccgc
    15241 aagccttact ggagtttgct aaagtcaatc ccagcacggt tacctatccg cgcccaccgg
    15301 actttaccgg cacggcgttt cttgaacagt tgctgattat gctgacgccc gatcccgcag
    15361 cattaaaaga agcgccggac gatgcgactt tcgcccgtgt cactgctccc ttgtggcaat
    15421 atcttgatgc gctacatccg tatttgtggc gcgaaggaaa ggatttcccg ccttcgcccg
    15481 cgcggatgga tgctctgctg aaagccggca cattgcgcct gtcgctgacc tttaaccccg
    15541 cgcatgcgca gcaaaaaatc gccagcggcg atttgccggc aagcagttac agttttggct
    15601 ttcgcgaggg gatgattggc aacgtgcatt tcgtcaccat tccagccaac gcgaatgcca
    15661 gtgctgcggc gaaggtagtt gccaatttcc tgctctcacc cgatgcgcaa ctgcgtaaag
    15721 cagatcccgt tgtctggggc gatccttctg ttctcgatcc gcaaaaactg cctgatgggc
    15781 agcgcgaaac attgcaatca agaatgccgc aagatctgcc gccggtactg gctgaaccgc
    15841 acgcaggttg ggtaaatgcg ctggaacaag aatggctaca ccgttacggt acgcattaat
    15901 ctttttgctg tgggcgatgg tggcggtgat ttatgcacca ctgatcccgg cagctctcac
    15961 gctgatatcg cctgctttgt cgttgacaca ctggcaggcg ttatttgccg atccacagtt
    16021 accgcaggca ttactggcaa cgctggtgtc gacaaccatc gcggcggtcg gggcgttgtt
    16081 gattgccctg ctggtgattg tggcgctgtg gccttggccg aaatggcagc gtatgtgtgc
    16141 ccgtctgccg tggctgctcg ccattcccca tgtggctttt gccaccagcg cccttctgct
    16201 ctttgctgag ggagggctgc tttatgacta tttcccgtat ttcactccgc taatggacaa
    16261 attgggtatt gggctgggcc tcacccttgc agttaaagaa agcgcatttc tgctgtggat
    16321 cttagcggca gtattgagcg aaaaacggct gttgcagcag gtcattgtgc tggattcgct
    16381 gggctacagc cgctggcaat gcctgaactg gctgctcttg ccctccgtcg cacctgcact
    16441 ggcaatggcg atgttggcga ttgttgcctg gtcgctttcg gtcgtggatg tggcgattat
    16501 tcttgggcca ggtaatccac cgacgctggc agtaattagc tggcagtggt taactcaggg
    16561 cgacgcggat caacaaacga aaggcgcact cgccagtctg ctgctgatgc tgttactcgc
    16621 cgcctacgtt ttgctgagct atctgctatg gcgcagctgg cggcgcacta ttccccgcgt
    16681 agatggcatt cgcaagcctg ccacaccttt attaccaggc actacgctgg cgagtttttt
    16741 acccttaacc ggtgtgctgt gtgtggttct gctggcgatc ctcgcggatc agtcgacgat
    16801 caatagtgaa gcactcatca acagcctgac aatggggctg gtggcgacat tcatcgcttt
    16861 gctcctgtta ctgctgtggc tggaatgggg gccacagcgt cgccagttgt ggctctggtt
    16921 gcccattcta ttgcccgctc tgccactggt ggcgggccag tacacgctgg cgctatggct
    16981 gaacctggat ggaagctgga cggcggtggt ctgggggcat ctgctgtggg taatgccgtg
    17041 gatgctgttt atcctgcaac ccgcctggca gcgcattgat tcacggttaa ttttgattgc
    17101 gcaaacactg agctggtcgc gggccaaaat cttcttttac gtgaaatgtc cgcttatgct
    17161 acgccctacg ctgattgcct tcgcggtggg attttcagtc agtattgcgc agtatatgcc
    17221 gacgctgtgg ctgggcacgg ggcgttttcc gacgctcacc actgaggcgg tggcattaag
    17281 cagcggcggc agcaatggta ttttcgccgc ccaggcttta tggcaactgc tattaccgct
    17341 tattattttt gccctgaccg cgttagtcgc aaaatgggta ggttatgtca gacaaggact
    17401 ccgctaatgc tctgcgtgaa aaatgtttcg ctacgtttac cagaaagccg cttgctgaca
    17461 aacgttaact ttacggtgga taaaggtgac attgtcacgt taatggggcc gtctggctgt
    17521 ggaaaatcca ctctgttttc atggatgatt ggtgcactgg ctggacagtt ttcttgtaca
    17581 ggtgagctat ggctcaatga gcaacggatt gacatgctac ccaccgcaca gcgtcagatt
    17641 ggcattcttt tccaggatgc actgttattt gaccagttca gcgtcgggca aaatttactg
    17701 ctggcgctac cgtcgacgct caaggggact gccaggcgca atgccgtgaa agacgcgctt
    17761 gatcgagcgg gactggctga aacttatcac caggaccctg cgacattatc aggcggtcag
    17821 cgagcgcggg tggccctgct gcgcgccctt ctcgctcagc caaaagcgtt actcctggat
    17881 gagccattca gccgtcttga tgtggctctg cgcgataatt ttcgccagtg ggtgttcagc
    17941 gaagttcgcg aactggcgat ccccgtcgtt caggtaacgc acgatctcca ggatgttcct
    18001 gctgatagtt ctgttctgga tatggagcag tggtcagaaa attacaacaa actgcgataa
    18061 cgcaaagttt ttctcaatgc gtcagttcag aatggcgcac tcaaaactac aatgtcggga
    18121 ttttcgatga aacgtgtttc tcaaatgacc gcgctggcaa tggctttagg gctggcttgc
    18181 gcttcttcgt gggccgctga actggcgaag cctcttacac ttgaccagct tcaacaacaa
    18241 aatggcaaag cgatagatac tcgcctcagc gcgttttata acggctggcc acaaacctta
    18301 aatggccctt ctggtcatga acctgccgcc ttaaacctct ctgccagctg gctcgacaaa
    18361 atgagcaccg aacagctcaa cgcgtggatc aagcaacata acctgaaagc cgatgccccg
    18421 gtggcgctgt acggtaatga caaagatgtc gacgccgtca aaacgcgact gcaaaaagca
    18481 ggttttacgc atatctccat tctgagtgac gcgctaagcg aaccttcccg tctacaaaaa
    18541 ctgccgcact ttgagcagct ggtttatccg caatggctgc acgacctgca acaaggtaaa
    18601 gaggttacgg cgaaacctgc cggtgactgg aaagtcattg aagcggcctg gggcgctcct
    18661 aagctttacc ttatcagcca tattcccggt gctgactaca tcgataccaa cgaagtggaa
    18721 agcgaaccgc tgtggaacaa agtttctgat gaacaactaa aagcgatgct ggcaaaacac
    18781 ggcattcgcc atgacaccac ggtcattctg tacgggcgtg acgtatacgc tggagcgcgt
    18841 gtggcgcaaa ttatgctcta cgcaggcgtg aaagatgtgc gtctgctgga tggcggctgg
    18901 caaacctggt ccgacgcggg actgcctgtt gagcgaggaa cgccaccgaa agtgaaagcg
    18961 gaaccggatt tcggcgtgaa gatcccggca caaccgcagt tgatgctcga tatggaacag
    19021 gcccgtggac tgctgcatcg ccaggatgca tcgctggtga gcattcgttc gtggccagaa
    19081 tttatcggta cgaccagcgg ttacagctat attaaaccaa aaggtgaaat agccggagca
    19141 cgttggggac acgctggtag cgactcgacg catatggaag attttcataa cccggatggc
    19201 accatgcgca gcgccgatga tattaccgct atgtggaaag cgtggaatat caaaccagag
    19261 cagcaagttt cattctactg cggcaccggc tggcgcgcgt ccgaaacctt tatgtacgca
    19321 cgcgccatgg gttggaagaa tgtttccgtg tatgacggcg gctggtacga atggagcagc
    19381 gatccaaaaa atccggtagc aaccggtgaa cgcggcccgg acagtagcaa ataacattgc
    19441 aattactgac gctggagcga cttcagcgtc agatacccac tccacacccg cgtaaaggtc
    19501 gtcatccagc acaacgcacc gaagatccac gcaaaccacg caaaccatgc agggaataaa
    19561 catcccagca caaacagtaa gatcgtttct gtgccttcgg ttaacccgcc cagataataa
    19621 aacgatttat gcgcataacc gggattatca atctgatgtt tcgccgccag cgcagcaaaa
    19681 gcgagaaaac tgctgcctgt gccgataaac gcaaacagca gccagccgcc tgccagggcg
    19741 ttttgctgcg gtgcggcgag gataaaccca aaaggtacca gcgcgtaaaa aagaaaatcg
    19801 agagaaatat caagaaagcc gcccgcgtcg gtaagccctc tgcgccgcgc cagcgcaccg
    19861 tccagaccat caagcagcct gttcaacaaa atgacgacaa gcgccgccag ataccagccc
    19921 agcgccagaa aaggcagtgc cagcacgccg atggcaaatc ccactaatgt taaaccatct
    19981 ggcgtaatgc ccggtttatc aagaacccgc acgcactgat gcaacaacgg tttaatccgg
    20041 ggatgaagat ggcggtctag cacaggcact ccttaaatat aaagcctttc tgattgagca
    20101 acagtgcgga tattatggca tttttcgctt atctgcccgt gtgtaattta tgaaaatgat
    20161 tgaagttgtt gccgccatca ttgaacgtga tggcaaaatt ttactcgcgc aacgccccgc
    20221 ccagagcgat caggcgggat tatgggagtt tgccggtggt aaagtcgagc cggatgaaag
    20281 ccagcggcag gcgctggtgc gtgagttacg cgaagaactg ggcatcgaag caactgtggg
    20341 tgagtatgtt accagccatc agcgagaagt ttcgggccgg attatccatc ttcatgcctg
    20401 gcacgtaccc gacttccacg ggacgttaca ggcacatgaa catcaggcgc tggtctggtg
    20461 ctcacctgaa gaggcgctgc aatatccgct ggcccctgct gacattccat tattagaggc
    20521 gtttatggct ttacgcgccg ccagaccagc ggattagtgc taagggtttt gtcatcccgc
    20581 tggcattgca gcagtattcc ctcggcttta attaccgcgc cttcagaata atttttatcc
    20641 tgataaacgc agcactgagt acagggctgc gctgactgcc cgcctgaact gaacacttct
    20701 ggcggtacgt ttacctccac gtccggacga taatgcgggt tagccagtgc aattaatgga
    20761 aatgctaata ctacggcgaa caatgctcga ctcacaggga agtccttaac gttattgtct
    20821 ctgctactga taacggtagc cgggtggcaa aactttagcg tctgagttat cgcatttggt
    20881 tatgagatta ctctcgttat taatttgctt tcctgggtca tttttttctt gcttaccgtc
    20941 acattcttga tggtatagtc gaaaactgca aaagcacatc acataaacaa cataagcaca
    21001 atcgtattaa tatataaggg ttttatatct atggatcaga catattctct ggagtcattc
    21061 ctcaaccatg tccaaaagcg cgacccgaat caaaccgagt tcgcgcaagc cgttcgtgaa
    21121 gtaatgacca cactctggcc ttttcttgaa caaaatccaa aatatcgcca gatgtcatta
    21181 ctggagcgtc tggttgaacc ggagcgcgtg atccagtttc gcgtggtatg ggttgatgat
    21241 cgcaatcagg tacaggtcaa ccgtgcatgg cgtgtgcagt tcagctctgc catcggcccg
    21301 tacaaaggcg gtatgcgctt ccatccgtca gttaacctct ccattcttaa attcctcggc
    21361 ttcgaacaaa ccttcaaaaa tgccctgact accctgccga tgggcggtgg taaaggcggc
    21421 agcgatttcg atccgaaagg aaaaagcgaa ggtgaagtga tgcgtttttg ccaggcgctg
    21481 atgactgaac tgtatcgcca cctgggcgcg gataccgacg ttccggcagg tgatatcggg
    21541 gttggtggtc gtgaagtcgg ctttatggcg gggatgatga aaaagctctc caacaatacc
    21601 gcctgcgtct tcaccggtaa gggcctttca tttggcggca gtcttattcg cccggaagct
    21661 accggctacg gtctggttta tttcacagaa gcaatgctaa aacgccacgg tatgggtttt
    21721 gaagggatgc gcgtttccgt ttctggctcc ggcaacgtcg cccagtacgc tatcgaaaaa
    21781 gcgatggaat ttggtgctcg tgtgatcact gcgtcagact ccagcggcac tgtagttgat
    21841 gaaagcggat tcacgaaaga gaaactggca cgtcttatcg aaatcaaagc cagccgcgat
    21901 ggtcgagtgg cagattatgc caaagaattt ggtctggtct atctcgaagg ccaacagccg
    21961 tggtctgtac cggttgatat cgccctgcct tgcgccaccc agaacgaact ggatgttgac
    22021 gccgcgcatc agcttatcgc caatggcgtt aaagccgtcg ccgaaggggc aaatatgccg
    22081 accaccatcg aagcgactga actgttccag caggcaggcg tactgtttgc accgggtaaa
    22141 gcggctaatg ctggcggtgt agcaacgtca ggccttgaaa tggcgcaaaa cgctgcgcgc
    22201 ctgggctgga aagccgagaa agttgacgca cgtttgcatc acatcatgct ggatatccac
    22261 catgcctgtg ttgagcatgg tggtgaaggt gagcaaacca actacgtgca gggcgcgaac
    22321 attgccggtt ttgtgaaggt tgccgatgcg atgctggcgc agggtgtgat ttaagttgta
    22381 aatgcctgat ggcgctacgc ttatcaggcc tacaaatggg cacaattcat tgcagttacg
    22441 ctctaatgta ggccgggcaa gcgcagcgcc cccggcaaaa tttcaggcgt ttatgagtat
    22501 ttaacggatg atgctcccca gggaacattt cttatgggcc aacggcattt cttactgtag
    22561 tgctcccaaa actgcttgtc gtaacgataa cacgcttcaa gttcagcatc cgttaacttt
    22621 ctgcgatagc agcagatatg ccagtaaaga aatcccatta gactattttt ttgataatct
    22681 tcttcgcttt cgaacaactc gtgcgccttt cgagaagcaa gcattatata atgccaggcc
    22741 agttcttctt caattgtccc gttttgaaaa gctgtgcttg atatcgagat catccatgat
    22801 aattccgccg cccatattag cttcgccgag gatttaccgg agcgattatt agcgcaatca
    22861 gagatatagt ctgagggaaa aacagcaaat ttattcaaca aggcgataac ctgctctggg
    22921 gcttcctcca tgtttgcttt aaaggtattg gctccatggt cgccagaaag aaaatgctcc
    22981 attaaggcac aataactttc gctatcttcg ataccccatt gatcctctaa agactcgcgt
    23041 cttttactta tgatatcgat cgagtcaaag ggaagcacat gatattggaa ggtatctttg
    23101 ccaggttcag gctttcgcgg ccagaactcc agcgtttcag accattgctt atgatagaac
    23161 cgataaggtg cgatcaattg tagcgcctgt aacttctcga tactgagcgg ctcaatacct
    23221 ttagcctgat aataatgcag ttgttctttt tttgctttaa aaccggcccg aacaataagc
    23281 cccatcataa ttaatagata aagaaaagag catcccgcag taatcaggcc tctttcattc
    23341 aaaccgttgg atgttatcgc tgcgaataca aacattacag cgacaacaca tgttaaatac
    23401 cccccccact tacaaagcag catggcctta ttttctttaa tcatccgttc aaaattacta
    23461 ttaaatattt cccagccatt aaaagaatac ttctcgctcc caggatggtt ttgtaataaa
    23521 acttttttca tcacattccc tgttattaca tactttagag gcgataattt tatccatgca
    23581 aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt tcagaatatg actcgaatag
    23641 cacgaaagat tcgctcgctt acgctatcgc cccgcttccg acttcatctg ctggcggact
    23701 ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc caccactgcc
    23761 cggagccaca atgccgcgaa actgccgcac cggcgtacgt ttggcttgat caataagctg
    23821 atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct tttcgctgat
    23881 ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtcgcca tttctggcag
    23941 cgaatggaat aacgcttttc cggcgtcggt agagtggata tagcgccctt ttttggtcag
    24001 aaaaccacgc ttgaacagca gttcaataat cccggcacgg gttgcttctg tccccagacc
    24061 atcggtcgca cgaaggatct ttttcagatc tttgtcctgc acaaagcgcg caatcccggt
    24121 catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt gccgctctat
    24181 cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca atggcgtgcc
    24241 gtcgttttct tcatcgcgct ctttgctgcc taacagcgcg cgccaacctg cttcagcaag
    24301 aaaacgcgct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa cacacttgcg
    24361 gaacactgca tccgggcaga actgcatcag atactgacgg gcaatcaggt tatagacctt
    24421 cgcttcgttc tccgtcaggt tgatcgcaga actccttgcg gtcggaatga tggcgtggtg
    24481 cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta ccactggctg
    24541 cggcaacaga tctggtgcat gaacgctgat ggcattcatc accgcgtggc gtccggcaaa
    24601 atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattaact tgtgtgtttc
    24661 gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccaaagc gttttgccgc
    24721 ttcaatctgc aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt cccgtttatc
    24781 gttatagctg gtgacaatag ccggttgacc actaatgcgg ttaaccacat gctccgccag
    24841 tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt cgctcggttg
    24901 ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgcgctttga cttcaaagaa
    24961 gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc cgagcacggg
    25021 tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac cgagaatggt
    25081 atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg ccagcgcaga
    25141 aacgcacaat ggcacaaact cactgttgga acgaagacgg tcgatcgccc gctcaaccgc
    25201 ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt ccggtgccag
    25261 ttgcagatag tccagcactt catccaccag caattgccct tcacgatccg ggtccccggc
    25321 gtgaacgatt tcgctggctt catgcaggaa gcgtttgatg acgttaagtt gtttggtcac
    25381 ggagggtcgg ggctgtaatt gccacttttc cgggacaatc ggcaaatccg caagattcca
    25441 gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt gaccgataca
    25501 ccaggtcacc acctgaccat taccgcactc gataaagcca tcgcctttcc ggtgcggttt
    25561 gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa acaaccgcat
    25621 tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt tcgccaatcg
    25681 ccgtcagttc aatgccaaac tcggcggctg tagttttaac ctcattttct gcttccggca
    25741 tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg cgcacttcac
    25801 gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcagtg ccgccaggta
    25861 ctgcgcccaa cttaatgtac tcttcaacac cggggagttt cgggatcgct tcatagtcga
    25921 cgcgtgcctg cacaccagcc ccctgacaca tttcgctcaa gtggcccagc agaccaaagc
    25981 ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg cctgcgatgt
    26041 tcatccggca catcacttcc gtcgccagtc cctgatgttc tggtttcaac agtgattttt
    26101 tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt ttgcatccgg
    26161 cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt accgccagac
    26221 caaaaatcgg ctccggcgca tcgatggagt gaccgccagc cagcgcaata cccgcctgac
    26281 ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa agtttgttaa
    26341 tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg aagatatcgc
    26401 tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg atcggcataa
    26461 agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc gccgcatcgt
    26521 cgcgggtctc attacccaca agcaaattcg gatcaacaaa cttcgcctgc tcactgtgca
    26581 ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg tggctgtatt
    26641 gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat ccgggtatgg
    26701 taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc tttgctcaca
    26761 atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg ggtccgggac
    26821 gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac cgacaatttt
    26881 atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta atgcgccact
    26941 gcgccagatg ccgccaaatc cttgggcaac ggctgccatt tgcatcgcca tgaccgcgca
    27001 tccggcagac atttcctgtt cccatcgcgg gactttatga ttttcttcgc atttcgccac
    27061 caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga tagctttgtc
    27121 atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct cgcgcccttc
    27181 cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg cacgcatacc
    27241 cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca ggcgggaggc
    27301 gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg tcgtgatatt
    27361 tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa ttcctgctga
    27421 caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca ggtgtgacct
    27481 taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt ttaaatggac
    27541 gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta ttttcctcgt
    27601 actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag aaacggccag
    27661 tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaacccg acagttctca
    27721 gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc tgcaggaaaa
    27781 ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca atatcaccgg
    27841 tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc agtacatcgg
    27901 caaagcccta aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg gcgagaacta
    27961 cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt caccgcaagg
    28021 cgtggttgat ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt tgctggataa
    28081 gctgaaagtt tccacccatg tgttccgcgt gggtacgtat aaatctgccg ttgaaccgtt
    28141 tattcgtgat gatatgtcac cggcagcccg cgaagctgac agccgctgga ttggtgagct
    28201 gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc agcaggtatt
    28261 ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata ccgcgaaata
    28321 tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg aaaaagctct
    28381 gaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca gttattacga
    28441 ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct tcgctaatgg
    28501 cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca ctgcggcaca
    28561 aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg ttaatagccc
    28621 aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag cccgggcagc
    28681 gggtaagcct gcggttgtat cgatgggcgg catggcggca tctggtggtt actggatttc
    28741 cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta tcggtatctt
    28801 cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata ctgatggtgt
    28861 ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg aagcgcagca
    28921 gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg ttgctgatgc
    28981 gcgtcattcg acgccggagc aaattgataa aatcgcccag ggccacgtct ggaccggtca
    29041 ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg cggttgccaa
    29101 agcagcagag ctggcaaaag tgaaacagtg gcatctggaa tactacgttg atgaaccgac
    29161 cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt tgccagatgc
    29221 gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta aaagtgaaag
    29281 cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc tgacctgcgc
    29341 caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat tattttttac
    29401 ctcgacgaat cctcttcccg ctgtctcgcc ccatatttcc ttatccgcat agtatcaggt
    29461 gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat atcaatatca
    29521 tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg cagcgttccg
    29581 agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg atgccggaat
    29641 tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgttg atggattctt
    29701 ctgatatgac gccggaagac tggcagcata ttgctgaaga tattaaagcg cactatgacg
    29761 actatgatgg ttttgtcatt ctacacggta ccgacacgat ggcgtatacc gcctctgcgc
    29821 tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca caaatcccgc
    29881 tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac gttgcggcga
    29941 attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc ggcaaccgca
    30001 ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt cctccgttac
    30061 tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcac ggtgaagggg
    30121 aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt tatccaggga
    30181 tttctgctga cgtcgtgcgc aattttctgc gccaaccggt gaaagcattg attctgcgct
    30241 cctatggcgt gggtaatgcg ccacaaaaca aagccttcct gcaggaatta caagaagcca
    30301 gcgatcgcgg tattgtggtg gtcaacctga cacaatgtat gtccggtaaa gtgaatatgg
    30361 gtggttatgc caccggtaac gccctcgccc atgccggcgt tattggcggt gcagatatga
    30421 ctgtagaagc cacgctaacc aaactgcatt acctgctgag ccaggaactg gatactgaaa
    30481 ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat gattaaggag
    30541 actgcaatgc cccatcgcgc cctgttactg gtcgatttac aaaatgattt ctgtgctggt
    30601 ggcgcgctcg ccgtgccgga aggtgacagt acggtagatg tcgctaaccg cctgattgac
    30661 tggtgccagt cgcgcggtga agcggttatc gccagtcagg actggcaccc ggcgaatcac
    30721 ggcagttttg ccagtcagca cggtgtagag ccttatacgc caggccaact cgacggtttg
    30781 ccacaaacct tctggccaga tcactgtgtg cagaacagtg aaggcgcaca attacatccg
    30841 ttactgaacc aaaaagcgat cgcagcggtg ttccataaag gcgaaaatcc tttagttgac
    30901 agttacagtg ccttttttga taacggccgt cggcagaaaa cctctctcga tgactgggta
    30961 cgcgatcatg aaatcgatga attgatcgtt atgggcctgg ctactgacta ttgcgtgaag
    31021 tttaccgtgc tggacgcgtt acagttaggt tataaggtaa acgtgattac cgatggttgt
    31081 cgtggcgtga atatccagcc ccaggacagt gcgcacgcgt ttatggagat gtcagcagct
    31141 ggggcaacgc tatatacgct ggcagactgg gaagagacgc aggggtaata ttagttgaat
    31201 tcatctctgg cctacatttc ctcaatgaca ttgtaggcca gataatactt gtcagtgttg
    31261 catttaacct acttgcagct aaaacacgac gtcctaacaa gtatatttgc cctcccttaa
    31321 taacaggctt aaatactaga taactcattc acttaaacat agcaataggc tttgctgaga
    31381 taccaaaatc ttcttttaac tgctgtttgc tcttcatgac catctgacca tttgtgtcaa
    31441 ttgtcatatg ctgtgcttcg gtattatggc gggcttgcca cagcatcacc agttgcaggc
    31501 aattctcttt ttgttcctcg gtcaacgcaa cgccatcagg ccatttcccc agttcaacgg
    31561 cggtcgacaa acgctggtat acctcaggcg tcatgctgtt gataatgtca tcaagattca
    31621 taatttttcc gctcctgtgg aataagttgc tgaatcgttt ttcaaccgtt gatttcttcg
    31681 ccgttttcgc catcagtgaa gcgtaaagaa gcagagttaa cacaataacg ttcgcctgtt
    31741 ggttgcggcc cgtcgggaaa gacatgcccc agatgggcat cacagttacc gcaacgaatt
    31801 tctatgcgct gcattccatg tgacaagtct ttgatataac gaatggattc ttcactcacc
    31861 ggttcgtaga aactgggcca gccacagccg gaatcatact tggtttcgga atgaaacagc
    31921 ggggcatcgc agatcaaaca gtgatatacg ccgtcacgct tgttatgcag taaacgaccc
    31981 gtaaatggcg gttctgtccc atgattctgc gtcacgtaaa actgcatctc ggacaaattt
    32041 tttttcagtt cttctgccga aggtttatta gccatttgct cacatctcac tttaatcgtg
    32101 ctcacattac gtgactgatt ctaacaaaac attaacacca actggcaaaa ttttgtccta
    32161 aacttgatct cgacgaaatg gctgcaccta aatcgtgatg aaaatcacat ttttatcgta
    32221 attgcccttt aaaattcggg gcgccgaccc catgtggtct caagcccaaa ggaagagtga
    32281 ggcgagtcag tcgcgtaatg cttaggcaca ggattgattt gtcgcaatga ttgacacgat
    32341 tccgcttgac gctgcgtaag gtttttgtaa ttttacaggc aaccttttat tcactaacaa
    32401 atagctggtg gaatatatga ctatcaaagt aggtatcaac ggttttggcc gtatcggtcg
    32461 cattgttttc cgtgctgctc agaaacgttc tgacatcgag atcgttgcaa tcaacgacct
    32521 gttagacgct gattacatgg catacatgct gaaatatgac tccactcacg gccgtttcga
    32581 cggtaccgtt gaagtgaaag acggtcatct gatcgttaac ggtaaaaaaa tccgtgttac
    32641 cgctgaacgt gatccggcta acctgaaatg ggacgaagtt ggtgttgacg ttgtcgctga
    32701 agcaactggt ctgttcctga ctgacgaaac tgctcgtaaa cacatcaccg ctggtgcgaa
    32761 gaaagtggtt atgactggtc cgtctaaaga caacactccg atgttcgtta aaggcgctaa
    32821 cttcgacaaa tatgctggcc aggacatcgt ttccaacgct tcctgcacca ccaactgcct
    32881 ggctccgctg gctaaagtta tcaacgataa cttcggcatc atcgaaggtc tgatgaccac
    32941 cgttcatgct actaccgcta ctcagaaaac tgttgatggc ccgtctcaca aagactggcg
    33001 cggcggccgc ggcgcttccc agaacatcat cccgtcctct accggtgctg ctaaagctgt
    33061 aggtaaagta ctgccagaac tgaatggcaa actgactggt atggcgttcc gcgttccgac
    33121 cccgaacgta tccgtagttg acctgaccgt tcgtctggaa aaagctgcaa cttacgagca
    33181 gatcaaagct gccgttaaag ctgctgctga aggcgaaatg aaaggcgttc tgggctacac
    33241 cgaagatgac gtagtatcta ccgatttcaa cggcgaagtt tgcacttccg tgttcgatgc
    33301 taaagctggt atcgctctga acgacaactt cgtgaaactg gtatcctggt acgacaacga
    33361 aaccggttac tccaacaaag ttctggacct gatcgctcac atctccaaat aagttgagat
    33421 gacactgtga tctaaaaaga gcgacttcgg tcgctctttt ttttacctga taaaatgaag
    33481 ttaaaggact gcgtcatgat taagaaaatt tttgcccttc cggtcatcga acaaatctcc
    33541 cctgtcctct cccgtcgtaa actggatgaa ctggacctca ttgtggtcga tcatccccag
    33601 gtaaaagcct cttttgcatt acagggcgca caccttctct cgtggaaacc tgcgggtgaa
    33661 gaagaagttc tgtggttgag caacaacaca ccgttcaaaa atggcgtcgc tattcgcggt
    33721 ggcgtaccgg tttgctggcc gtggtttggt ccggcggcac aacaaggtct gcctgcgcac
    33781 ggttttgccc gcaacctgcc gtggacgctg aaatcacatc gtgaagatgc tgatggcgta
    33841 gcgctgactt ttgaattgac acaaagcgaa gagacgaaaa aattctggcc gcacgacttt
    33901 acgctgttag cgcatttccg cgtgggtaaa acctgtgaaa tcgatcttga atcacatggc
    33961 gaatttgaaa ccacctctgc cctgcatacc tactttaacg tgggtgatat cgctaaggta
    34021 agcgtcagtg ggctgggcga tcgcttcatt gataaagtaa atgacgcgaa agaagatgta
    34081 ctgaccgacg gtattcagac cttccctgac cgtaccgatc gcgtgtatct gaatccgcaa
    34141 gattgcagcg tgattaatga tgaagcgctg aatcgtatta tcgccgtagg ccaccagcat
    34201 catctgaacg ttgtcggctg gaacccggga ccggcgcttt cagttagcat gggcgacatg
    34261 ccggatgatg gctacaaaac atttgtttgt gtagaaacgg cttacgcttc agaaacgcaa
    34321 aaagtgacca aagagaaacc tgcacatctg gcgcaatcca ttcgcgttgc gaaacgttaa
    34381 tttgcgttaa tgttgtgtgc cgggtgcgat gcatccggca cgtatcaaca tcacaccata
    34441 tccagcgcag tttttccttt tggtgccgga tacgctttat ccagcatggt taattccgct
    34501 gacgaaagtt cgacctcaag cacagccgca ttctgttgga catgggcaat cgtggccgct
    34561 tttggaatcg ccatcacacc ctgatggctg attacccacg ccaacaatac ttgtgccgcg
    34621 ctgatattgt gagcatgtgc aatttcgttg actaccgcgt tttttaacag tccattgcgc
    34681 aaccgcccgg cctgggctaa tggactgtaa gccatcaccg gcatctgctg ttgctggcac
    34741 caggggagta gatcgtactc aatccctcgt gaaccgagat ggtaaagcac ctgattagtg
    34801 gcacactgat ttccccccgg cagctgccag agttcctgca tatcagcatg atcaaggtta
    34861 gaaacgcccc agcggcggat ttttccctgg gcgatcaatt tttccatcgc tgccacagtc
    34921 tcttcaaaag cgaaactgcc agaccagtgt aataagtaga gatcgagata atcagtattg
    34981 agacggcgta aactggcttc gcatgcattt atcgcttttt gcccgccagc attccacgga
    35041 tagactttag agacgagaaa gacgttatcg cgcagaccgg ttaatgcttc cccaaccacc
    35101 ttttcggcac cgccatcggc atacatttcg gcggtatcaa tgagggttaa accgagctca
    35161 atgcccgcgc gtagtgcagc aacttctgtt ttgcgctgac tggcatcttc gcccatatac
    35221 catgttccct gccctatggc tggcagtgag acatcgccac taaattgaat cattttttgt
    35281 tgcattgttt cctcccaggt attgcaccac cgtaatgcaa aacagggcgt gacgccctgt
    35341 ttttatgcac aaaatgccct ggaaagatgc attatcagaa tttgtaggtg atcccggtag
    35401 aaatcaggcc agtccaggat ttatccacca tcgggctgtc agtaacttca tcagacagac
    35461 gggtatagcg cgcggtaccg taaacactcc agtcgccgag gaagttgtag ctggcgctca
    35521 gctccaggta agggctccag ctgtcgttcg ggttatagcc acgcagaccg ctgcgagcgg
    35581 actctttgcg cgatacgcca taatagtatt cgttctggtt ttcgctgttc cactgcacac
    35641 cgatacccgg ggtcacggtc aggccgccgt tggtgtaacg atacaaccag gccatatccc
    35701 agacgatgcc gttgctgtta tccagggtat cgccagccag ggtggtacgc aggtaaccgt
    35761 actgggtaaa gtgagcataa gacagaccag ccatcatggt gctcttacgg tcatccaggt
    35821 gacgcatttg gtgatcgcca ctgtctttcg ctttgaagta aagcggcgac caataagcgg
    35881 taattgaaag tttgtccgtt gcgtcattcc acaggtagta accaccacct aagccacgga
    35941 accagaagtt atcgccttca tagttgatta ccggtactgg gtaaacatcg gtatcgtaat
    36001 ctttatatgg gtgctcaacg acacctacgc ctgcgcccag ggaaaattta ccttcagcgt
    36061 gcgctacgcc tgcagacgtt gcgataagca ctccaagtgc cagaagtttg agtttggtca
    36121 caattaatca ttccttaaac aaatgtttag cgggcgacaa agtttacccg tcaatacatc
    36181 gaaacccaac ctttttacgt tttcattttt taaagtaact gtttaatttt cctgacgcgg
    36241 atgacaccgc gcttacagcc aaatgaattt agcgttactg gctaccctgg tctttacatt
    36301 aattatgcaa aatttatgga tgagttgttg atatgccatt gaaattaaga aagccgtgca
    36361 ggcaagtttt ccatttgcca tctacgctta attttgaagg tgtatcaccg ggcacgttgt
    36421 tctcatcgtc gataaaatgg catgagagtt gctgtgtttt agcaagagac gtcgttcagt
    36481 ttacctcttc cgggagcctc tactattcat atgaacggct cttaacctgt gctaaaaaac
    36541 gaaaggacgg cataccatga atatattcga tcactatcgc cagcgatatg aagctgccaa
    36601 ggacgaagag ttcacactgc aggagtttct taccacttgt cggcaagatc gcagtgctta
    36661 tgccaacgcg gctgagcggc tattgatggc tatcggtgag cctgtcatgg tcgatacagc
    36721 ccaggaaccc agactttctc gactcttttc taaccgggtc attgcacgtt atccggcatt
    36781 tgaagagttt tacggcatgg aagacgcgat tgaacagatt gtctcttatc tgaaacacgc
    36841 ggctcagggg ctggaagaga agaaacaaat cctgtatctg ctggggcctg tgggtggggg
    36901 taaatcatcg cttgctgagc gactgaaatc attaatgcag ctcgtaccga tttatgtatt
    36961 gagcgccaac ggtgagcgta gcccggtcaa cgatcatccg ttctgtcttt tcaatccgca
    37021 ggaagatgct cagattctgg aaaaagagta tggcattcct cgccgttatc tcggcaccat
    37081 catgtcgcca tgggcggcaa aacgcctgca tgaatttggt ggcgatatca ctaagttccg
    37141 ggtagtgaag gtctggccgt caattctgca acaaattgct atcgccaaaa cggaacccgg
    37201 tgatgagaac aaccaggaca tctccgcgct ggttgggaaa gtcgatattc gtaaactcga
    37261 acactacgcg cagaatgacc cggacgccta cggctattcc ggtgcgctgt gccgcgccaa
    37321 ccaagggatc atggaatttg tcgagatgtt taaagcaccg ataaaagtgc tgcatccgct
    37381 gttaaccgcc actcaggaag gtaactacaa cgggacggaa ggtatctccg ccctgccgtt
    37441 caacgggatt attctcgccc actcgaacga atccgaatgg gtcactttcc gtaataacaa
    37501 aaacaacgaa gccttcctcg accgtgttta catcgtgaag gtgccgtatt gcttacgcat
    37561 ttccgaagag atcaaaatct acgagaaatt gcttaatcac agtgaattga ctcacgcccc
    37621 ctgcgcccct ggcacgctgg aaacgctgtc acgtttttcc attctttcgc gccttaaaga
    37681 gccagaaaac tccagcattt attcaaagat gcgggtttat gatggcgaaa gtctgaaaga
    37741 caccgatccc aaagccaagt cgtatcagga atatcgtgac tacgccggtg tcgatgaagg
    37801 gatgaacggt ctgtcgacgc gttttgcgtt taagatcctc tcccgcgtgt tcaacttcga
    37861 tcatgtagaa gtggcggcaa acccggtcca tctgttctac gtactggaac agcagatcga
    37921 gcgcgagcag ttcccacaag agcaggcaga acgctatctg gagttcctga aaggttatct
    37981 gatcccgaaa tatgccgagt ttatcggcaa agagatccag acggcctacc ttgaatccta
    38041 ttccgaatat gggcaaaaca ttttcgaccg ttatgttacc tacgcggatt tctggattca
    38101 ggatcaggag tatcgcgatc cggataccgg gcagctgttt gaccgcgagt ctcttaacgc
    38161 cgagctggag aaaatcgaga aaccggcggg gatcagtaat ccaaaagatt tccgcaacga
    38221 gattgttaac ttcgtactgc gcgccagagc gaataacagc ggacgcaatc cgaactggac
    38281 cagctatgaa aaactgcgca cggttatcga gaagaaaatg ttctccaata ccgaggagct
    38341 gttgccggtt atctcgttta acgccaaaac gtcaaccgac gagcagaaga aacacgacga
    38401 ctttgtcgac cgtatgatgg aaaaaggcta cacccgtaaa caggtgcgtt tactgtgcga
    38461 atggtatttg cgcgtacgta aatcgtctta acaaccctgg cccggtacac atgttaccgg
    38521 gcctacaacg acagcgaacc gtgggcctga gaagcggcaa cacaggcgta gcatacagtt
    38581 ggcaaatgta gtacgggggg catatgacct ggtttattga ccggcgtctg aacggcaaaa
    38641 acaaaagcat ggtgaatcgc cagcgttttt tacgccgtta taaagcgcaa attaaacagt
    38701 cgatctccga ggccattaat aagcgttcgg tgactgacgt cgacagcggc gaatccgtat
    38761 ccattcccac ggaagatatt agcgaaccga tgtttcatca ggggcgtggc ggtctgcgcc
    38821 accgcgtgca tccgggcaat gaccatttcg tccagaacga ccgaattgaa cgtccccagg
    38881 gtggcggcgg aggttccggc agtggtcagg gccaggccag ccaggatggt gaaggtcagg
    38941 atgaatttgt ctttcagatt tcgaaagatg agtatcttga tttgctcttt gaagatttgg
    39001 ccttaccgaa tctgaaacaa aaccaacaac gccagctgac cgaatataaa acgcatcggg
    39061 cgggttatac cgctaacggc gttccggcaa atatcagcgt tgtgcgctcc ttgcagaact
    39121 cactggcgcg acgcacagcc atgacggcag gcaagcggcg ggaacttcat gcactggaag
    39181 agaatttggc catcatcagc aacagtgaac ctgcgcagct gctggaagag gaacgtctgc
    39241 gcaaagaaat tgcagaatta cgtgccaaaa ttgaacgcgt cccttttatt gacaccttcg
    39301 atttacgtta caagaactac gagaagcggc ccgatccctc cagccaggca gtgatgtttt
    39361 gcctgatgga cgtttccggt tcaatggatc aatccactaa agatatggct aagcgttttt
    39421 atattctgct gtatctgttc ctcagcagaa cgtataagaa cgtggaagtc gtatacatcc
    39481 gccatcatac ccaggcgaaa gaagtcgatg aacatgagtt tttctactcg caggaaacag
    39541 gcggcaccat cgtttccagc gccctgaaac tgatggatga ggtagtgaaa gagcgttata
    39601 acccggcaca gtggaatatt tacgctgcac aagcatcgga cggcgataac tgggccgatg
    39661 actctccgct ttgccatgaa atcctggcga aaaaattatt acctgttgtt cgttattaca
    39721 gctatatcga aattacccgt cgtgcacatc agacattgtg gcgagaatat gagcatctgc
    39781 aatctacttt cgacaacttt gcgatgcagc acatccgcga ccaggatgat atttatccgg
    39841 tgttccgtga actgtttcat aaacaaaatg caacagctaa agactaaaac tatcagccag
    39901 gtcattatcg cctggctgat ttttagctta ctgtaaatta tctcatttat tacatacatt
    39961 agcttacaat cgctttaaat atgacagcat aacctttaca taatttag
//
LOCUS       DAZGXZ010000029        39380 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000029 DAZGXZ010000000
VERSION     DAZGXZ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.029"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            182..532
                     /gene="hypA"
                     /locus_tag="ACHKJF_001653"
     CDS             182..532
                     /gene="hypA"
                     /locus_tag="ACHKJF_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0949032.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            536..1408
                     /gene="hypB"
                     /locus_tag="ACHKJF_001654"
     CDS             536..1408
                     /gene="hypB"
                     /locus_tag="ACHKJF_001654"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP0949033.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            1399..1671
                     /gene="hypC"
                     /locus_tag="ACHKJF_001655"
     CDS             1399..1671
                     /gene="hypC"
                     /locus_tag="ACHKJF_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP0949034.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            1671..2792
                     /gene="hypD"
                     /locus_tag="ACHKJF_001656"
     CDS             1671..2792
                     /gene="hypD"
                     /locus_tag="ACHKJF_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP0949035.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            2789..3799
                     /gene="hypE"
                     /locus_tag="ACHKJF_001657"
     CDS             2789..3799
                     /gene="hypE"
                     /locus_tag="ACHKJF_001657"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP0949036.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            3873..5951
                     /gene="flhA"
                     /locus_tag="ACHKJF_001658"
     CDS             3873..5951
                     /gene="flhA"
                     /locus_tag="ACHKJF_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP0949037.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(5988..6341)
                     /locus_tag="ACHKJF_001659"
     CDS             complement(5988..6341)
                     /locus_tag="ACHKJF_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP0949038.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(6373..6552)
                     /locus_tag="ACHKJF_001660"
     CDS             complement(6373..6552)
                     /locus_tag="ACHKJF_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949039.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            6628..9189
                     /gene="mutS"
                     /locus_tag="ACHKJF_001661"
     CDS             6628..9189
                     /gene="mutS"
                     /locus_tag="ACHKJF_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP0949040.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            9295..9951
                     /gene="pphB"
                     /locus_tag="ACHKJF_001662"
     CDS             9295..9951
                     /gene="pphB"
                     /locus_tag="ACHKJF_001662"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0949041.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPRSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(10002..10769)
                     /gene="ygbI"
                     /locus_tag="ACHKJF_001663"
     CDS             complement(10002..10769)
                     /gene="ygbI"
                     /locus_tag="ACHKJF_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP0949042.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            10965..11873
                     /gene="ltnD"
                     /locus_tag="ACHKJF_001664"
     CDS             10965..11873
                     /gene="ltnD"
                     /locus_tag="ACHKJF_001664"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP0949043.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            11870..13132
                     /gene="otnK"
                     /locus_tag="ACHKJF_001665"
     CDS             11870..13132
                     /gene="otnK"
                     /locus_tag="ACHKJF_001665"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP0949044.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            13129..13767
                     /locus_tag="ACHKJF_001666"
     CDS             13129..13767
                     /locus_tag="ACHKJF_001666"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0949045.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            13772..14548
                     /locus_tag="ACHKJF_001667"
     CDS             13772..14548
                     /locus_tag="ACHKJF_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP0949046.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            14637..16001
                     /locus_tag="ACHKJF_001668"
     CDS             14637..16001
                     /locus_tag="ACHKJF_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP0949047.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(16040..16276)
                     /locus_tag="ACHKJF_001669"
     CDS             complement(16040..16276)
                     /locus_tag="ACHKJF_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP0949048.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(16287..17714)
                     /locus_tag="ACHKJF_001670"
     CDS             complement(16287..17714)
                     /locus_tag="ACHKJF_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP0949049.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIVLHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(17714..18307)
                     /locus_tag="ACHKJF_001671"
     CDS             complement(17714..18307)
                     /locus_tag="ACHKJF_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP0949050.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            18454..18861
                     /locus_tag="ACHKJF_001672"
     CDS             18454..18861
                     /locus_tag="ACHKJF_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0949051.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(18982..19974)
                     /gene="rpoS"
                     /locus_tag="ACHKJF_001673"
     CDS             complement(18982..19974)
                     /gene="rpoS"
                     /locus_tag="ACHKJF_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP0949052.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(20037..21131)
                     /gene="nlpD"
                     /locus_tag="ACHKJF_001674"
     CDS             complement(20037..21131)
                     /gene="nlpD"
                     /locus_tag="ACHKJF_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP0949053.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(21316..21942)
                     /gene="pcm"
                     /locus_tag="ACHKJF_001675"
     CDS             complement(21316..21942)
                     /gene="pcm"
                     /locus_tag="ACHKJF_001675"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP0949054.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(21936..22697)
                     /gene="surE"
                     /locus_tag="ACHKJF_001676"
     CDS             complement(21936..22697)
                     /gene="surE"
                     /locus_tag="ACHKJF_001676"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP0949055.1"
                     /translation="MRILLSNDDGVHAPGIQTLEKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(22678..23727)
                     /gene="truD"
                     /locus_tag="ACHKJF_001677"
     CDS             complement(22678..23727)
                     /gene="truD"
                     /locus_tag="ACHKJF_001677"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP0949056.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(23724..24203)
                     /gene="ispF"
                     /locus_tag="ACHKJF_001678"
     CDS             complement(23724..24203)
                     /gene="ispF"
                     /locus_tag="ACHKJF_001678"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP0949057.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(24203..24913)
                     /gene="ispD"
                     /locus_tag="ACHKJF_001679"
     CDS             complement(24203..24913)
                     /gene="ispD"
                     /locus_tag="ACHKJF_001679"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP0949058.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(24932..25243)
                     /gene="ftsB"
                     /locus_tag="ACHKJF_001680"
     CDS             complement(24932..25243)
                     /gene="ftsB"
                     /locus_tag="ACHKJF_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP0949059.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(25437..25760)
                     /locus_tag="ACHKJF_001681"
     CDS             complement(25437..25760)
                     /locus_tag="ACHKJF_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP0949060.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(25810..26415)
                     /gene="cysC"
                     /locus_tag="ACHKJF_001682"
     CDS             complement(25810..26415)
                     /gene="cysC"
                     /locus_tag="ACHKJF_001682"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP0949061.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(26415..27842)
                     /gene="cysN"
                     /locus_tag="ACHKJF_001683"
     CDS             complement(26415..27842)
                     /gene="cysN"
                     /locus_tag="ACHKJF_001683"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP0949062.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(27844..28752)
                     /gene="cysD"
                     /locus_tag="ACHKJF_001684"
     CDS             complement(27844..28752)
                     /gene="cysD"
                     /locus_tag="ACHKJF_001684"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP0949063.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            29004..30041
                     /gene="iap"
                     /locus_tag="ACHKJF_001685"
     CDS             29004..30041
                     /gene="iap"
                     /locus_tag="ACHKJF_001685"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP0949064.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   30123..30822
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=30123..30151
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(30919..31212)
                     /gene="cas2e"
                     /locus_tag="ACHKJF_001686"
     CDS             complement(30919..31212)
                     /gene="cas2e"
                     /locus_tag="ACHKJF_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP0949065.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGKNRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(31212..32132)
                     /gene="cas1e"
                     /locus_tag="ACHKJF_001687"
     CDS             complement(31212..32132)
                     /gene="cas1e"
                     /locus_tag="ACHKJF_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP0949066.1"
                     /translation="MTFVPLSPIPLKDRTTMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(32129..32779)
                     /gene="cas6e"
                     /locus_tag="ACHKJF_001688"
     CDS             complement(32129..32779)
                     /gene="cas6e"
                     /locus_tag="ACHKJF_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP0949067.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELRTGQSLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(32761..33507)
                     /gene="cas5e"
                     /locus_tag="ACHKJF_001689"
     CDS             complement(32761..33507)
                     /gene="cas5e"
                     /locus_tag="ACHKJF_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP0949068.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(33518..34573)
                     /gene="cas7e"
                     /locus_tag="ACHKJF_001690"
     CDS             complement(33518..34573)
                     /gene="cas7e"
                     /locus_tag="ACHKJF_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP0949069.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKVLAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(34585..35121)
                     /gene="casB"
                     /locus_tag="ACHKJF_001691"
                     /gene_synonym="cse2"
     CDS             complement(34585..35121)
                     /gene="casB"
                     /locus_tag="ACHKJF_001691"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP0949070.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(35118..36680)
                     /gene="casA"
                     /locus_tag="ACHKJF_001692"
                     /gene_synonym="cse1"
     CDS             complement(35118..36680)
                     /gene="casA"
                     /locus_tag="ACHKJF_001692"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP0949071.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(36778..>39380)
                     /gene="cas3"
                     /locus_tag="ACHKJF_001693"
     CDS             complement(36778..>39380)
                     /gene="cas3"
                     /locus_tag="ACHKJF_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP0949072.1"
                     /translation="DYHLLCWHSLDVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQ
                     FFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQ
                     ELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIKP
                     LFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQ
                     KTLIQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILE
                     DVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRP
                     SLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVG
                     TLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVI
                     LLSATLSQQQRDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDV
                     ERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLS
                     LFHSRFAFSDRQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAP
                     VDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNS
                     AFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKY
                     YCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGA
                     HAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYS
                     ATEGLIGKVG"
BASE COUNT         9292 a        10650 c        10062 g         9376 t
ORIGIN      
        1 gctttaagca ttttttgtgc caactttcaa tttattgtta ttaaagagat ttttaagcta
       61 aagatgaatt tcgtcgccgt gtcgacgtgt catttcgaca tcatcgacat tattcaccgc
      121 agggataatc aacactggca caattattgc ttgtagctgg caatagttaa tgggaggcga
      181 tatgcacgaa ataaccctct gccaacgggc actggaattg atcgaacagc aggccgcaaa
      241 acacggcgca aaacgcgtaa ctggggtctg gctcaaaatt ggcgcatttt cttgtgtcga
      301 aaccagctct cttgcctttt gttttgatct ggtttgccgc ggcagcgtgg cggaaggttg
      361 taaactgcac ctcgaagaac aagaggccga atgctggtgt gaaacatgcc aacagtatgt
      421 gacgctactg acccagcgcg tccgccgctg tccacagtgt catggtgaca tgctacagat
      481 tgtggcagac gacggtttac agattcggcg gatagaaata gaccaggagt gagcgatgtg
      541 tacaacatgc ggttgcggtg aaggcaacct gtatatcgag ggtgatgaac ataaccctca
      601 ttccgcgttt cgtagcgcgc catttgcccc ggcggcacgc ccgaagatga aaatcaccgg
      661 cattaaagcg cctgaattta cccccagcca gactgaagaa ggcgacctgc attacggtca
      721 tggcgaagcg ggcactcacg cgccgggcat gagccagcgt cggatgctgg aagtcgaaat
      781 tgacgtgctg gacaaaaata accgtctggc tgaacgcaac cgcgcgcgct ttgctgcccg
      841 caagcaactg gtgctcaacc tggtttccag ccctggttcc ggtaaaacca ccctgctgac
      901 ggaaacctta atgcgcctga aagacagcgt tccgtgcgca gttattgaag gcgatcagca
      961 aaccgtgaac gatgccgcac gcattcgcgc taccggcaca ccagcgattc aggtgaacac
     1021 tggtaaaggc tgccaccttg acgcacagat gattgccgac gccgcaccgc gtctgccact
     1081 ggacgataac ggtattctgt ttatcgaaaa cgttggcaac ctcgtatgcc cggccagctt
     1141 cgatctcggt gaaaaacaca aagtagcggt cctttccgtt accgaaggtg aagacaaacc
     1201 gctgaaatat ccgcatatgt ttgccgccgc ctcgctgatg ctgctcaaca aagttgacct
     1261 tttgccgtat ctcaactttg acgttgagaa gtgcatcgcc tgcgcacgcg aagtcaatcc
     1321 agaaattgaa atcatcctta tttccgccac cagcggcgaa gggatggacc agtggctgaa
     1381 ctggctggag acacagcgat gtgcataggc gttcccggcc agatccgcac cattgacggc
     1441 aaccaggcga aagtcgacgt ctgcggcatt cagcgcgatg tcgatttaac gttagtcggc
     1501 agctgcgatg aaaacggtca gccgcgcgtg ggccagtggg tactggttca cgttggcttt
     1561 gccatgagcg taattaatga agccgaagca cgcgacactc tcgacgcctt acaaaacatg
     1621 tttgacgttg agccggatgt cggcgcgctg ttgtatggcg aggaaaaata atgcgttttg
     1681 ttgatgaata tcgcgcgccg gaacaggtga tgcagttaat tgagcatctg cgcgaacgtg
     1741 cttcacatct ctcttacacc gccgaacgcc ctctgcggat tatggaagtg tgtggcggtc
     1801 atacccacgc tatctttaaa ttcggcctcg accagttact gccggaaaac gttgagttta
     1861 tccacggtcc gggttgtccg gtgtgtgtac tgccgatggg tagaatcgac acctgcgtgg
     1921 agattgccag ccatccggaa gtcatcttct gtacctttgg cgacgccatg cgcgtaccag
     1981 ggaaacaggg atcgctgttg caggcaaaag cacgcggtgc cgatgtgcgc atcgtttact
     2041 cgccgatgga tgcgttgaaa ctggcgcagg agaatccaac ccgtaaagtc gtattcttcg
     2101 gcttaggttt tgaaaccacc atgccgacca ccgccatcac cctgcaacag gcgaaagccc
     2161 gcgatgtgca gaatttttac ttcttctgcc agcatattac gcttatcccg acgctgcgca
     2221 gtttgctgga acagccggat aacggtatcg acgcgttcct cgcgccgggc cacgttagta
     2281 tggttatcgg cactgatgcc tataatttta tcgccagcga ttttcatcgt ccgctggtgg
     2341 tcgctggttt cgaacccctt gatctactac aaggcgtggt catgctggtg gagcagaaaa
     2401 tagcggccca cagcaaggta gagaatcagt atcgtcgagt ggtaccggat gccggtaacc
     2461 tgctggcgca acaggcgatt gccgatgtgt tctgtgtcaa cggcgacagc gaatggcgcg
     2521 gcttaggcgt gattgaatct tctggcgtac acctgacgcc ggattatcaa cgattcgatg
     2581 ccgaagcaca tttccgcccg gcaccgcagc aggtctgcga tgacccgcgc gcacgttgtg
     2641 gtgaagtatt aacgggcaaa tgtaagccgc atcaatgccc gctgtttggt aacacctgta
     2701 atcctcaaac cgcatttggt gcactgatgg tctcctccga aggagcgtgc gccgcgtggt
     2761 atcagtatcg tcagcaggag agtgaagcgt gaataatatc caactcgccc acggcagcgg
     2821 cggccaggcg atgcagcaat taatcaacag cctgtttatg gaagcctttg ccaacccgtg
     2881 gctggcagag caggaagatc aggcacgtct tgatctggcg cagctggtag cggaaggcga
     2941 ccgtctggcg ttctccaccg acagttacgt cattgacccg ctgttctttc ctggcggtaa
     3001 tatcggcaag ctggcgattt gcggcactgc gaatgacgtt gcggtcagtg gcgctattcc
     3061 gcgctatctc tcctgtggct ttatcctcga agaaggattg ccgatggaga cactgaaagc
     3121 cgtagtgacc agcatggcag aaaccgcgcg cacggcaggc attgccatcg ttactggcga
     3181 tactaaagtg gtgcagcgcg gcgcggcaga taaactgttt atcaacaccg ctggcatggg
     3241 cgcaattccg gcgaatattc attggggcgc acagacgcta accgcaggcg atgtgttgct
     3301 ggtgagcggc acgctcggcg accacggggc gactatcctt aacctgcgtg agcagctggg
     3361 gctggatggc gaactggtca gcgactgcgc ggtgctgacg ccgcttattc agacgctgcg
     3421 tgacattccc ggcgtgaaag cgctgcgtga tgccacccgt ggtggtgtaa atgcggtggt
     3481 tcatgagttc gcggcagcct gcggttgcgg tattgaaatt tctgaatcag cgctgccggt
     3541 taaacctgcc gtgcgcggcg tttgcgaatt gctgggactg gacgccctga actttgccaa
     3601 cgaaggcaaa ctggtgatcg ccgttgaacg caacgcggca gagcaagtgc tggcagcgtt
     3661 acattcccat ccactgggga aagacgcggc gctgattggt gaagtggtgg aacgtaaagg
     3721 tgttcgtctt gccggtctgt atggcgtgaa acgaaccctc gatttaccac acgccgaacc
     3781 gcttccgcgt atatgctaat aaaattctaa atctcctata gttagtcaat gaccttttgc
     3841 accgctttgc ggtgctttcc tggaagaaca aaatgtcata tacaccgatg agtgatctcg
     3901 gacagcaagg gttgttcgac atcactcgga cactattgca gcagcccgat ctggcctcgc
     3961 tgtgtgaggc tctttcgcaa ctggtaaagc gttctgcgct cgccgacaac gcggctattg
     4021 tgttgtggca agcgcagact caacgtgcgt cttattacgc atcgcgtgaa aaagacaccc
     4081 ccattaaata tgaagacgaa actgttctgg cacacggtcc ggtacgcagc attttgtcgc
     4141 gccctgatac gctgcattgc agttacgaag aattttgtga aacctggccg cagctggccg
     4201 caggtgggct atacccaaaa tttggtcact attgcctgat gccactggcg gcagaagggc
     4261 atatttttgg tggctgtgaa tttattcgtt atgacgatcg cccctggagc gaaaaagagt
     4321 tcaatcgtct gcaaacattt acgcagatcg tttctgtcgt caccgaacaa atccagagcc
     4381 gcgtcgtcaa caatgtcgac tatgagttgt tatgccggga gcgcgataac ttccgcatcc
     4441 tggttgccat taccaacgcg gtgctttccc gcctggatat ggacgaactg gtcagcgaag
     4501 tcgccaaaga aatccattac tatttcgaca ttgacgatat cagtatcgtc ttacgcagcc
     4561 accgtaaaaa caaactcaac atctactcca ctcactatct tgataaacaa catcccgccc
     4621 acgaacagag cgaagtcgat gaagccggaa ccctcaccga acgcgtgttc aaaagtaaag
     4681 agatgctgct gatcaatctc cacgagcggg acgatttagc cccctatgaa cgcatgttgt
     4741 tcgacacctg gggcaaccag attcaaacct tgtgcctgtt accgctgatg tctggcgaca
     4801 ccatgctggg cgtgctgaaa ctggcgcaat gcgaagagaa agtgtttacc actaccaatc
     4861 tgaatttact gcgccagatt gccgaacgtg tggcaatcgc tgtcgataac gccctcgcct
     4921 atcaggaaat ccatcgtctg aaagaacggc tggttgatga aaacctcgcc ctgaccgagc
     4981 agctcaacaa tgttgatagt gaatttggcg agattattgg ccgcagcgaa gccatgtaca
     5041 gcgtgcttaa acaagttgaa atggtggcgc aaagtgacag taccgtgctg atcctcggtg
     5101 aaactggcac gggtaaagag ctgattgccc gtgcgatcca taatctcagt gggcgtaata
     5161 atcgccgcat ggtcaaaatg aactgcgcgg cgatgcctgc cggattgctg gaaagcgatc
     5221 tgtttggtca tgagcgtggg gcttttaccg gtgccagcgc ccagcgcatt ggtcgttttg
     5281 aactggcgga taaaagctcc ctgttcctcg acgaagtggg cgatatgcca ctggagttac
     5341 agccgaagtt gctgcgtgta ttgcaggaac aggagtttga acgtctcggc agcaacaaaa
     5401 tcattcagac ggacgtgcgt ctaatcgccg cgactaaccg cgatctgaaa aaaatggtcg
     5461 ccgaccgtga gttccgtagc gatctctatt accgcctgaa cgtattcccg attcacctgc
     5521 cgccactacg cgagcgtccg gaagatattc cgctgctggc gaaagccttt accttcaaaa
     5581 ttgcccgtcg tctggggcgc aatatcgaca gcattcctgc cgagacgctg cgcaccttga
     5641 gcaacatgga gtggccgggt aacgtacgcg aactggaaaa cgtcattgag cgcgcggtat
     5701 tgctaacacg cggtaacgtg ctgcagctgt cattgccaga tattgtttta ccggaacctg
     5761 aaacgccgcc tgccgcaacg gttgtcgccc aggagggcga agatgaatat cagttgattg
     5821 ttcgcgtgct gaaagaaacc aacggcgtgg ttgccgggcc taaaggcgct gcgcaacgtc
     5881 tggggctgaa acgcacgacc ctgctgtcac ggatgaagcg gctgggaatt gataaatcgg
     5941 cattgattta actgcaaatt gccggacaga tctgcctgtc cggcatacta ttcacgaggt
     6001 tttttcggac gatatttttc cggcagttct ggcaccggac gcttgtcatc gatgagatga
     6061 cgcacggtta agatcggatg acgccacagc attctcggcc cggcccaacg cataatctgt
     6121 ttcatctctt cacgctttgc aggctggtaa cagtgcaccg gacactgctt acaggctggt
     6181 ttctcttcgc cgaacacaca tttatccagc cgcttttgcg cgtaaacaaa caacgcctcg
     6241 taatgctccg gctccgctga cgcctgcggg catttcgctt gataaagatc gatcattttt
     6301 ttaatcgtca gtttttcacg agagatacgc ttgccggaca tgctgcctcc acctcattaa
     6361 gatgtattta tattacatct taatcttaaa gggcactatg actctaaaga agaaaggttc
     6421 gccaaccgac gcaattttgc gtacttgctt cataagcatc acgcaaaagc tgcaaaacag
     6481 catctttccc ggaaccagca tcaagaactc accgttcgct tcttcccctg aaatgattaa
     6541 ctccggtatc atgtgcgcct tatgtgatta caacgaaaat aaaaaccatc acaccccatt
     6601 taatatcagg gaaccggaca taaccccatg agtgcaatag aaaatttcga cgcccatacg
     6661 cccatgatgc agcagtatct caggctgaaa gcccagcatc ccgagatcct gctgttttac
     6721 cggatgggtg atttttatga actgttttat gacgacgcaa aacgcgcgtc gcaactgctg
     6781 gatatttcac tgaccaaacg cggtgcttcg gcgggagagc cgatcccgat ggcggggatt
     6841 ccctaccatg cggtggaaaa ctatctcgcc aaactggtga atcagggaga gtccgttgcc
     6901 atctgcgaac aaattggcga tccggcgacc agcaaaggtc cggttgagcg caaagttgtg
     6961 cgtatcgtta cgccaggcac catcagcgat gaagccctgt tacaggagcg tcaggacaac
     7021 ctgctggcgg ctatctggca ggacagcaaa ggtttcggct acgcgacgct ggatatcagc
     7081 tccgggcgtt ttcgcctgag cgaaccggcc gaccgcgaaa cgatggcggc agagctgcaa
     7141 cgcactaatc cggcggaact gctgtatgca gaagatttcg ccgagatgtc gctgattgaa
     7201 ggccgtcgcg gcctgcgccg tcgcccgctg tgggagtttg aaatcgacac cgcgcgccag
     7261 cagttgaatc tgcaatttgg cacccgcgat ctggtcggtt ttggcgtcga gaacgcgccg
     7321 cgcggacttt gtgctgccgg ttgtctgttg cagtatgcga aagataccca acgcacgacc
     7381 ctgccgcata ttcgttctat caccatggaa cgtgagcagg acagcatcat tatggatgcc
     7441 gcgacacgtc gtaacctgga aattactcag aacctggcgg gcggtgcgga aaatacgctg
     7501 gcttctgtgc tcgactgcac cgtcacgccg atgggtagtc gtatgctgaa acgctggctg
     7561 catatgccag tgcgcgatac ccgcgtgttg cttgagcgcc agcaaactat tggcgcattg
     7621 caggatttca ccgccgagtt gcagccggta ctgcgtcagg tcggcgacct ggaacgtatt
     7681 ctggcacgtc tggctttacg aactgctcgc ccacgcgatc tggcccgtat gcgccacgct
     7741 ttccagcaac tgccggagct gcgtgcgcag ttagaaactg tcgatagtgc accggtacag
     7801 gcgctacgtg agaagatggg cgagtttgcc gagctgcgcg atctgctgga gcgagcaatc
     7861 atcgacacac cgccggtgct ggtacgcgac ggtggtgtta tcgcatcggg ctataacgaa
     7921 gagctggatg agtggcgcgc gctggctgac ggcgcgaccg attatctgga gcgtctggaa
     7981 gtccgcgagc gtgaacgtac cggcctggac acgctgaaag ttggctttaa tgcggtgcac
     8041 ggctactaca ttcaaatcag ccgtgggcaa agccatctgg cacccatcaa ctacatgcgt
     8101 cgccagacgc tgaaaaacgc cgagcgctac atcattccag agctaaaaga gtacgaagat
     8161 aaagttctca cctcaaaagg caaagcactg gcactggaaa aacagcttta tgaagagctg
     8221 ttcgacctgc tgttgccgca tctggaagcg ttgcaacaga gcgcgagcgc gctggcggaa
     8281 ctcgacgtgc tggttaacct ggcggaacgg gcctataccc tgaactacac ctgcccgacc
     8341 ttcattgata aaccgggcat tcgcattacc gaaggtcgcc atccggtagt tgaacaagta
     8401 ctgaatgagc catttatcgc taacccgctg aatctgtcgc cgcagcgccg tatgttgatt
     8461 attaccggcc cgaacatggg cggtaaaagt acctatatgc gccagaccgc actgattgcg
     8521 ctgatggcct acatcggcag ctatgtaccg gcacaaaaag tcgagattgg acctatcgat
     8581 cgcatcttta cccgcgtagg cgcggcagat gacctggctt ccgggcgctc aacctttatg
     8641 gtggagatga ctgaaaccgc caatatttta cataacgcca ccgaatacag tctggtgtta
     8701 atggatgaga tcgggcgtgg aacgtccacc tacgatggtc tgtcgctggc gtgggcgtgc
     8761 gcagaaaatc tggcgaataa gattaaggca ttgacgctgt ttgccaccca ctatttcgag
     8821 ctgacccagc taccggagaa aatggaaggc gtcgccaacg tacatctcga tgcgctggag
     8881 cacggcgaca ctatcgcctt tatgcacagc gtgcaggacg gcgcggcgag caaaagctac
     8941 ggtctggcgg ttgctgctct ggcgggcgta ccgaaagagg ttattaagcg cgcacggcaa
     9001 aaactgcgtg agctggaaag catttcgccg aacgccgccg ctacgcaagt ggatggtacg
     9061 caaatgtctt tgctgtccgt accagaagaa acttcgcctg cggtcgaggc actggaaaac
     9121 ctggacccgg attcactgac tccgcgtcag gcgctggagt ggatttatcg cttgaagagt
     9181 ctggtgtaat aaaaattccc gatagtcttt tgctatcggg aatattaacg acaactgacg
     9241 aatcaaataa aaataccctg tataatagga aagcttattt tatagggtaa aaccatgcca
     9301 tctacacgct atcaaaaaat caatgcccat cactatcgcc atatatgggt cgttggtgat
     9361 attcatggtg aatatcagtt attacaatcc cgcttacatc aactctcttt tttccccgaa
     9421 accgacttac ttatttctgt cggcgataat attgatcgtg ggccggagag tcttgacgtc
     9481 ctgcgcctgc taaaccagcc ctggtttatc tccgttaaag gcaaccacga agcaatggcg
     9541 ctggatgcgt tcgcgaccgg cgatggcaat atgtggcttg ccagcggtgg tgactggttt
     9601 ttcgatttaa atgattcaga gcaaaaagaa gctacagatc tgttgctgaa attccatcac
     9661 cttccacata ttattgaaat cactaacgac aacataaaat atgtcatcgc acatgcagat
     9721 tatccgagga gtgaatatct ctttggtaaa gaaatagcgg agagcgaatt actctggcct
     9781 gttgatcgtg tgcagaaatc gcttaatggc gagttacaac aaataaacgg cgctgattat
     9841 tttatatttg gacatatgat gtttgataac attcagacgt tcgctaacca gatttatatt
     9901 gataccggat cgccgaaaag tgggcggctg tcattttata aaataaggta gtctcatgct
     9961 tcttctgtga agcatgagta accagatgtt attgcaggcc attattcatt tttcgctacc
    10021 agcaaagaga gatcctgctt cgccagcgcg cgactggcac tctccggcaa accgtcgtct
    10081 gtaataatct gatcaaactc gcttaatggt aacgccagcc atgtcgccac ctgaccatat
    10141 ttcgtcgcat cacagaccaa aactcgctgg cggctggcac tggcaatcgc ccgtttcacc
    10201 gtgactttat cttccgctgg cgtagaaatc ccccgcacac tccatgacga tgcagaaata
    10261 aaagcctgat caatcatcag gctgcgcagc atggtcgcag cggcttcccc gacacaggaa
    10321 cggttttccc gacacactgc accgccagtg tgaataattg tgcaattact gttgtcgagc
    10381 aagtagttcg caataacgaa atcgtttgtg accacagtca gtgactccat gtgaatcaga
    10441 tgctgtgcta tcgctaacgt ggtcgttccc gcatccagat agatacaact tcccggctga
    10501 acaagacttg ccgccagctt gccaatagcc gctttttgcg tcattgccag cgcagttttt
    10561 acctgatgag aaggttcatg cgccacgcgt cccggagact ggacgcctcc ggacaccagc
    10621 acaacggctc cctgctgctc cagtttttgt aaatcccgac gaatggtcat atgtgacaca
    10681 ttcattctgt ccgttagttc agcaatactg acaatgcctt tttcagctac catctcaagg
    10741 atgatttggc gacgctctac gggtatcaac ttttgctcct tcctttgtcc tgctgacatt
    10801 ctacgctatt tgcctgcgaa acgtgcgcgg cgcaactaac gcttagttca cataaaataa
    10861 cacacaatgt taatttatgt gaatcagctc accatatcgt tatcttccag cgcttatatt
    10921 cacaatatca aacaaaatat cacttaaatt aacaaggaga gcagatgaaa acgggatctg
    10981 agtttcatgt cggtatcgtt ggcttagggt caatgggaat gggagcagca ctgtcatgtg
    11041 tccgcgcagg tctttctacc tggggcgcag acctgaacag caatgcctgc gctacgttga
    11101 aagaggcagg tgcttgcggg gtttctgata acgccgcgac gtttgccgaa aaactggacg
    11161 ctctgctggt gctggtggtc aatgcggccc aggttaaaca ggtgctgttt ggtgaaacag
    11221 gcgttgcaca acatctgaaa cccggtacgg cagtaatggt ttcttccact atcgctagtg
    11281 ctgatgcgca agaaattgct accgctctgg ctggattcga tctggaaatg ctggatgcgc
    11341 cagtttctgg tggtgcagta aaagccgcta acggtgaaat gactgtcatg gcctccggta
    11401 gcgatattgc ctttgaacga ctggcacccg tgctggaagc cgttgccgga aaagtttatc
    11461 gcataggtgc agaaccggga ctaggttcga ccgtaaaaat tattcaccag ttgttagcgg
    11521 gcgtacatat tgctgccgga gccgaagcga tggcacttgc agcccgtgcg gggatcccgc
    11581 tggatgtgat gtatgacgtc gtgaccaatg ccgccggaaa ttcctggatg ttcgaaaacc
    11641 ggatgcgtca tgtggtggat ggcgattaca ccccgcattc agccgtcgat atttttgtta
    11701 aggatcttgg tctggttgcc gatacagcca aagccctgca cttcccgctg ccattggcct
    11761 caacagcatt gaatatgttc accagcgcca gtaacgcagg ttacgggaaa gaagacgata
    11821 gcgcagttat caagattttc tctggcatca ctctaccggg agcgaaatca tgatcaagat
    11881 tggcgttatc gccgatgatt ttaccggcgc gacggatatc gccagttttc tggtggaaaa
    11941 cggtctacca acggtacaaa ttaacggtgt tccaacaggt aaaatgccgg aagcaatcga
    12001 cgcactggtg atcagcctga aaacgcgctc ctgtccggtg gttgaagcca cacagcaatc
    12061 gctggcggct ctgagctggt tgcaacagca aggttgcaaa cagatctatt tcaaatactg
    12121 ctctactttc gacagtacgg cgaaaggtaa tatcggcccg gttaccgatg cattaatgga
    12181 tgctctcgac acgccgttta cggtcttctc tccggccctg ccggtcaacg gacgtacggt
    12241 ttatcagggg tatttgttcg taatgaatca actgctggcc gaatccggga tgcgccatca
    12301 cccggtaaat cccatgaccg acagctatct tccccgtctg gttgaagcgc aatccacagg
    12361 gcgctgcggc gtcgtttcgg cacatgtttt cgaacaaggt gtggatgccg ttcgtcaaga
    12421 gctggctcgc ttacagcaag agggctaccg ctacgcggtg cttgatgcgc tgaccgaaca
    12481 ccatctggaa attcagggag aagccttgcg cgatgcccca ctggtaacgg gcggttctgg
    12541 tctggcgatt ggcctggccc ggcagtgggc gcaagaaaac ggtaaccagg ctcgcgaagc
    12601 agggcatccg ctcgctgggc gcggcgtagt gctctccggt tcatgctctc aaatgaccaa
    12661 ccgccaggtg gcacattacc gtcaaattgc accagcccgt gaagttgatg tggcacgctg
    12721 cctctcaacc gaaactctgg ccgcttatgc acacgaactg gcagagtggg ttctgggcca
    12781 ggaaagtgta cttgctccac tggtttttgc caccgccagc actgacgcat tggcagcaat
    12841 tcaacagcaa tacggtgcac aaaaagccag tcaggcagta gaaactctat tttctcaact
    12901 agcggcgcgg ttagcagcgg aaggcgtgac acgctttatt gtcgcaggcg gtgagacctc
    12961 cggcgtagtc acacagagcc tgggcataaa agggtttcat attggcccaa ccatttcccc
    13021 cggcgtgccg tgggtaaacg cactggataa gcctgtctca ctcgccctta aatctggcaa
    13081 cttcggtgat gaagcctttt tttcacgagc ccaaagagag tttttatcat gagcgatttc
    13141 gcaaaagtag agcagtcttt gcgagaggag atgacgcgga ttgccagttc attctttcag
    13201 cgcggctatg caaccggttc ggctggcaat ctgtcgctgc ttttacctga cgggaattta
    13261 ctggcgacac cgacaggttc atgcctgggc aatctcgatc cgcagcggct ttccaaagtc
    13321 gccgcggatg gcgaatggtt aagtggtgac aaaccctcga aagaggtgct ctttcatctg
    13381 gcgctgtatc gcaacaatcc gcgctgtaaa gcggtggtgc atttgcacag cacatggtcg
    13441 acggcgcttt cctgcctgca agggctggac agcagcaacg ttattcgtcc gttcacacca
    13501 tacgtggtga tgcggatggg aaatgtcccg ctggtgcctt attaccgacc gggcgataaa
    13561 cgcatcgcac aggatctggc ggaactggca gcagacaatc aggctttttt actggcaaat
    13621 catggcccag tggtttgcgg tgaaagcctg caagaagccg ccaacaatat ggaagagctg
    13681 gaggaaacgg caaagctgat ttttattctc ggtgaccgcc cgatccgtta tctgaccgca
    13741 ggtgaaattg cggaattaag gagttaatgc aatgcctcgt tttgcagcta atttatccat
    13801 gatgttcacc gaagtgcctt ttattgaacg cttcgccgca gcgcgaaaag ccgggttcga
    13861 tgctgtggaa tttctgtttc cctatgacta ctccaccctg caaatccaaa agcaactgga
    13921 gcaaaaccat ctgacactgg cgctgttcaa taccgctccc ggagatatta atgcggggga
    13981 atggggatta tccgcccttc ccggacgtga gcacgaagca cacgcagaca tcgacctggc
    14041 actggaatat gcattggcgc tcaattgtga acaagtccat gtgatggcag gcgtcgtgcc
    14101 cgctggcgaa gacgctgaac ggtaccgggc agtatttatc gataatctcc gctacgctgc
    14161 cgaccgcttt gcgctacacg gcaagcgaat attagttgaa gcactcagcc ccgacgttaa
    14221 gcctcattat ctcttctcca gccagtatca ggcactggct attgttgaag aggttgcgcg
    14281 agataacgtg tttattcaac tagatacttt tcatgcgcaa aaagtagatg gcaacctgac
    14341 gcatttaatc cgcgactacg ctgggaaata tgcgcacgta caaattgccg gactacctga
    14401 tcgacatgaa ccggacgatg gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga
    14461 ggtgggatat cagggctgga tcggttgtga atataaacct cgtggcctca ccgaagaagg
    14521 gcttggctgg tttgacgcct ggcgctaatt cgttaaatcc ctaattacaa cgtacccata
    14581 catcccccca tctacggatg gggtaggtta actataactt tcagacaggg tttcccatgt
    14641 ccacaattac attgttatgc attgcgttag ctggcgtaat catgctgttg ctgctggtca
    14701 tcaaggcaaa ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg
    14761 cggcgggtat accggcgggc gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc
    14821 ttggttccgt cactattatt attggtctgg gcgctatgct ggggcgtatg atcgaacact
    14881 ctggtggtgc agagtcactg gctaattatt tcagtcgcaa gttaggtgac aaacgaacta
    14941 tcgctgcgct gactctggca gcgttcttcc tcggtattcc cgtcttcttt gatgtcggct
    15001 ttattattct tgcgccaatc atttacggtt ttgccaaggt tgccaaaata tcgccactca
    15061 aatttggcct gcctgtcgct ggaatcatgc taactgttca tgtggcggta ccgccgcatc
    15121 caggccctgt cgccgcagcg gggttactcc acgcagacat cggctggcta accatcatcg
    15181 gtattgcgat ttctattccc gtagggattg ttggctactt tgcagcgaaa ataatcaata
    15241 cgcgccaata tgcgatgtcg gtggaagtac tggaacagat gcaactggct ccggccagtg
    15301 aggaaggcgc aacaaaatta agcgataaaa taaatccacc gggcgtcgcg ctggtcacct
    15361 cgctaattgt tattcctatc gcgattatca tggcgggtac ggtttccgca acactgatgc
    15421 cgccttcgca tcccctgctt ggtacgctac agctgatcgg ctcaccaatg gtagctctaa
    15481 tgattgcgct ggtgctggca ttctggttat tggctttacg tcgcggctgg agcttacaac
    15541 acaccagcga cattatgggc tcagcccttc ccactgcggc ggtagtgatt ttggttactg
    15601 gtgctggagg ggtatttggc aaagtgctgg tggaatcggg cgtcggcaaa gcccttgcca
    15661 atatgctaca aatgattgac ctgcctctgt taccagccgc atttattatt tcactggctc
    15721 tgcgtgcatc gcaggggtca gcaaccgtag caatcctgac aaccggcggg ttactctcag
    15781 aagcggtgat gggattgaat ccgattcagt gcgtattggt gacgctggca gcctgctttg
    15841 gtgggcttgg cgcgtcacat attaatgact cagggttctg gattgtgacc aaatatctgg
    15901 ggttgtctgt agcagacggt ctgaaaacct ggacagtgtt aacgaccatt ctcggtttta
    15961 ccggattctt aattacctgg tgcgtatggg cggtaatttg accttaataa aaaggtccga
    16021 tgggcatcgg accttttatt taacgcttac cttccaccag cagcggcggg atgctcggca
    16081 ccattggcgc gtcatcaata tctttctgcg tcatgcggaa cgcttcggga taatgttcgc
    16141 ggctggtacg gcgcagcggt tcggtatcgc gccaggtata aaggcaatgc tggcactgat
    16201 ataccgtcca gacatctttc accggcgatt tcgccatcac ttcaatctgt tcatcggcac
    16261 aacgtggaca aatcatcttc ttctccttat ttacgtgcag ccagcatagc ggtcagtttt
    16321 tcagcccagg ctttggtttc cggtaaatcc accaccggtt ggctgtagtg accacggttg
    16381 tccggggcga caggcgtagt ggcgtcgata atcagcttgt cggtgatccc cgcagggctt
    16441 gagcctggat cgagttccag cacggacata ttcggcaact gcaccaaatc ccctgccggg
    16501 ttcactttcg aggagagcgc ccacatcacc tgcggcaggt tgaacgggtc aacgtcttca
    16561 tcgaccataa tcaccatctt cacgtagccc agaccatgcg gcgtggtcat tgcgcgcagg
    16621 cccaccgcgc gggcaaagcc gccgtagcgt tttttggtgg agataatcgc cagcaggcca
    16681 tgggtgtaca tggcgtttac cgcctgcact tcagggaact cggctttcag ctgctgatac
    16741 agcggcacgc aggtggctgg ccccatcagg tagtcgattt cggtccacgg cataccgagg
    16801 tacagcgatt cgaaaatcgg cctggtgcgg taagagactt tatcgatgcg caccacggtc
    16861 atgttacgcc cgccggagta gtgcccggta aactcaccga acggcccttc gatttcgcgt
    16921 ttacggcttt cgatgacccc ttcgaggatc acttctgaac cccacggcac atcaaaaccg
    16981 gtcaacgggg cggtggcgat cgggtacggg ctttcacgca gcgcgcctgc catttcgtac
    17041 tcggactgat catatttcag cggcgtggcc cccatcagcg tgatgatcgg atcgttaccg
    17101 agcgtgatcg caatcggcag atcttcaccg cgctcttctg ctttatgcag atgcaggacg
    17161 atatcgtgca tcggcaccgg ttgcaggccg agcttacgct tgcccttcac ttccatgcgg
    17221 tagatgccga cgttctgctt gccgaagtta tccgggtcga gcggatcgcg ggaaaccacg
    17281 cacgctttgt cgagatagaa accgccatcg ccatcgttta aacgaaacag cggcaggata
    17341 tcgaacaggt tgatctcgtc gccatcaacg gtgttctgcg cccaggctgg attggcgcgg
    17401 cgctccgggg caatcgggaa gttatcccag cggcggataa actcatcaat ctgcttttta
    17461 accggggcat ttggcggcag gccgagggaa atcgcgtggt tctgccagga accgatggtg
    17521 ttcatcgcca cgcgggcatc ggtaaagccg cgaatattat caaaccacag cgcgggcgcg
    17581 ccgtcgccga tacgcccggt ggcgttagct gctgctgcca gatccggctc ggcgttcact
    17641 tcttcgctga ttttcagtaa ctggccgtgg tcatcaagcg cctgtaaaaa gctgcgtaaa
    17701 tcatcaaatg ccattattca ttctcctgag aaaaattccg ggcctgcggc aatccttgcc
    17761 agcgcctggc gtggggatgt tcgaggccaa attgatccag cacgcgggct accacatggt
    17821 ggacaatgtc atctaccgtt tcgggatggt tataaaaggc aggcatcggc ggcaccatcg
    17881 ccacgcccat gcgtgaaagt gcgagcatat tttcgagatg gatggtgcta agcggcattt
    17941 cacgcggcac cagcaccagt ttgcggcctt ctttgagcac gacgtccgcc gcgcgcccta
    18001 ccaggccatc ggcgtaacca gcgcggatac cggcgagcgt tttcatactg cacggaataa
    18061 cgatcatgcc gtcggtacga aaagaaccgg atgagatgat cgccgcctga tccgccgggt
    18121 tatggctgaa gtctgcgagg gcagcaacat cgcgagcgct gtaaggcgtt tccagttcaa
    18181 tggtggtttt cgcccacttc gacatcacca gatgagtctc gacattcggc atctcccgca
    18241 gcgcttgcag taatgccaca ccaagaggcg caccggtagc ccctgtcatc ccgacgatca
    18301 gtttcattgc ttatcctctt aattgttcgt atacgaacgt tattattaac atactccttc
    18361 atttccacag cggcaaggat tgaaaagtaa caacatcttc ttcgtcacta aaaaaccgct
    18421 atgatagcga cagagaatta gcccggagtt tacatggcgt tacgaaataa agcgttccat
    18481 cagttacgac agctttttca gcagcacacg gctcgctggc agcacgagtt acctgacctg
    18541 accaaaccac agtacgcggt gatgcgcgcc attgctgata aacctggtat cgaacaggtg
    18601 gcactgatag aagcagctgt cagcaccaaa gcaacgttag cagaaatgtt ggcaagaatg
    18661 gaaaatcgtg gcttagtcag acgtgaacat gaccccgctg ataagcggcg acgctttgtc
    18721 tggttgactg ccgaaggcga aaagatactt gctgcggcga tacccattgg tgacagcgtg
    18781 gatgaggaat ttttggggcg tttgagtgga gaagagcagg agctgtttat gcagctggtg
    18841 cgcaagatga tgaacgcata gggggcaaat ttccggatgc gacgctatcg cgtcttatcc
    18901 agactacaaa tacaccgact caccaaccga aagaaaaaag gccagcctcg cttgagactg
    18961 gcctttctga cagatgctta cttactcgcg gaacagcgct tcgatattca gcccctgcgt
    19021 ttgcaggatt tcgcgcaaac ggcgcaggcc ttcaacctga atctggcgaa cacgttcacg
    19081 ggtgaggcca atttcacgac ctacatcttc cagtgttgcc gcttcgtacc ccagcaaacc
    19141 gaatcgacgt gccagcactt cacgctgttt ggcgttcagc tcgaacagcc atttgacgat
    19201 gctctgcttc atatcgtcat cttgcgtggt atcttccgga ccgttctctt tttcatcggc
    19261 caggatgtcc agcaacgctt tttcggaatc accacccagc ggggtgtcta ccgaggtaat
    19321 gcgctcgtta agacgaagca tacggctgac gtcatcaact ggcttatcca gttgctctgc
    19381 gatctcttcc gcacttggtt catggtccag cttatgggac aactcacgtg cggttcgcag
    19441 gtaaacgttc agctccttta cgatgtgaat cggcaaacga atagtacggg tttggttcat
    19501 aatcgcccgt tcaatcgtct ggcgaatcca ccaggttgcg tatgttgaga agcggaaacc
    19561 acgttccggg tcaaacttct ctaccgcgcg gatcagcccc aggttgccct cttcgataag
    19621 gtccagcaac gccagaccac gattgccata acggcgggca atttttacca ccagacgcaa
    19681 gttactctcg atcatccggc ggcgagaggc gacatctcca cgcagtgcgc gacgcgcaaa
    19741 ataaacttct tcttcggccg ttaacagtgg tgaataacca atctcaccaa ggtaaagctg
    19801 agtcgcgtcc aacacacgct gtgtggctcc ctgcgataac agttcctctt cggccaaatc
    19861 gttatcactg ggttcctctt ctactaaggc cttttcgtca aaaacctcaa ctccgttctc
    19921 atcaaattcc gcatcttcat ttaaatcatg aactttcagc gtattctgac tcataaggtg
    19981 gctcctaccc gtgatccctt gacggaacat tcaagcaaaa gcctggttcc gccgatttat
    20041 cgctgcggca aataacgcag cgggtttacg gatttcccct tgtaacgaat ttcaaaatgc
    20101 aagcgtgttg aactggttcc ggtgctaccc atggttgcta ttttttgccc cgccttcact
    20161 tcttgttgtt cccggaccag cattgtgtcg ttatgggcgt aggcactcag gtaatcatca
    20221 ttatgtttga tgataatcag attaccgtag ccgcgcagcg cgttaccagc ataaacaacg
    20281 cggccatctg cggtcgcgat aattgcctgt cctttgctgc ctgcgatatc aatccccttg
    20341 ttgcccccct cagaagcgcc aaaggtttcg atcactttgc cctcagtcgg ccagcgccag
    20401 gtggagatag gcgtactggt tgatgtactg ctgacagttg gctcggttgt gcttgctgtt
    20461 ggtaccgtta caggcgctgt gaccgtggtc gcagctggct tgttgttcgg caacattttg
    20521 ttagcactct gttcacccga agattcagaa tacgtaattg tcggttgcga cgcaacagca
    20581 acggtggaat tttgtgcagg cttgatcaca actccttgct ctgctgcgtc ggcctgggta
    20641 atggcatttc cgccagtgat tggcgtaccg gaagcattac ccacctgcaa ggtctgacca
    20701 acgttcagcg cgtatggtgc ctgaatattg ttgcgctgag caaggtcacg gaaatcgttg
    20761 ccagtaatcc aggcgatata gaaaagtgtg tcgccttttt tcacggtata ggtactgccg
    20821 ctataactgc ctttcggaat gttcccatac tgacggttat agacgatgcg tccgttttcc
    20881 atctgtaccg gctgctgagc tactggctgc actggctgga tttgcggttg ttgagtagcc
    20941 tgaatttgtg gctgctgtac cggctgaatt tgcggttgct gggctgtaga cgtcgtcccc
    21001 attttcggcg gcggcgtaat caacatacca gaattggtat ttgcaggcgc attgccatta
    21061 acggagctga ccggtgccgg tggatttgaa gtgtcagaac agcctgccag ccatagcgaa
    21121 accagtgaca aagccgcaat gcggcgaacg gtgaattttg ggcttcccgc gctcatttat
    21181 cccccaggaa aaattggtta ataaccagtg acataattac cgtgcaaggc accctactga
    21241 acactggaaa agatgttcac gatacgctga cctgcggcaa aataaccagg aaaaatccag
    21301 gtatttcctc acgttttaag ccagctcacc cttcactaaa gggacaaagc gcacggcctc
    21361 cacggtatcg ataataaatt cgcctccccg acgacgcacc cgtttcaaat actggtgctc
    21421 ctcccctacg ggtaagacga gaatcccgcc ttcgtccagc tgcgtcatta gcgcagttgg
    21481 aatttccggc ggtgccgccg taacaatgat agcgtcaaac ggcgcgcgtg cctgccaacc
    21541 ttgccatcca tcgccatgac gggttgaaac attatgtaaa tcaagatttt tcaggcggcg
    21601 acgcgcctgc cactgcaaac ctttaatccg ttcgaccgag caaacatgct ggacaagatg
    21661 cgccaggatt gccgtttgat atcccgaacc agtgccaatt tccagcaccc gcgactgcgg
    21721 cgtcagctcg agtaattcgg tcattcgcgc caccatatac ggctgcgaaa ttgtctgccc
    21781 ctgacctatc ggcaaggcga tattgtccca ggctttttgt tcaaacgctt catcaacgaa
    21841 tttttcacgc ggcacggcgg caagtgcatt cagcacctgc tcatcctgaa taccttgcgc
    21901 acgtaattga tccagaagtg cttgtacgcg tctgcttacc attgcgtgcc aactcccacg
    21961 ctgtttaacc agtctgaaac cacatcttgc gcgctatgcg cagttaaatc cacatgcagc
    22021 ggcgtgatgg agacatagcc ctcatctacc gcagcaaaat cggtccccgg accggcatca
    22081 catttaccgc ccggcgggcc aatccagtac agcgtattgc cgcgcggatc ttgctgcggg
    22141 atcacctgat ctgccggatg tcgtgtaccg cagcgcgtca cgcgaatacc tttgatttga
    22201 tccaagggta aatccggaac gttaatatta agaatacgcc cggtgcgcag cggctcttta
    22261 cacagtgcgc gcaaaattga acaggttacc gccgcggcag tgtcgtaatg tttatgcccg
    22321 tcaagcgaga cggcaagcgc cggaaaacct aaatgacggc cttccatcgc ggcggctacc
    22381 gtaccggaat aaataacatc atcccccaga ttcggcccgg cgttaattcc ggacacaaca
    22441 atgtccgggc gcggacgcat cagagcattc acgccaagat agacgcaatc ggtcggggtt
    22501 cccatttgca cagcaatatc accattttca aaggtaaacg tgcgcaggga ggattccagt
    22561 gtcagagaat ttgaagcgcc gctgcggtta cgatcggggg cgaccacctg aacgtcagca
    22621 aactcacgca aggctttctc cagcgtttgt ataccgggtg catgtacccc gtcatcatta
    22681 ctcagcaata tgcgcataat cacctgttgt gttgataagt tccctgacaa cgctggttgc
    22741 aaaactaccc gccggaagcc agaaacggat ctctacggtg acgtcatccc accaattcca
    22801 gcttaattgt tgcggataca gcagcatcgc tctgcgcgcg gcttcaactt tttcgcgcac
    22861 cagtaaagct tgtaattcag tttctgcggc gacagctgct tgttcgaatg ccagcgcttc
    22921 acgctgagtt ccccattcgc cactgcccgg caatgcggcg gttatcatca gctctttatc
    22981 gttgacgcga cgctgtaatt ccgccagttc ttcggtggtt gcgacaaacc agctaccacg
    23041 tccggctaat tgtagcgcat cgccgtcaac aacttgatta acgtctgctt ttttgaggcg
    23101 ctcagcaaca atctgattaa acaacgcact gcgggctgcc gacaaccaaa aactccgttt
    23161 attgcgatcg cgcaccggag tattggtttg cgcccagcgc tgagccccct gcaagttgct
    23221 accgccaatc ccaaaacgtt gggcaccgaa gtagttcggt acacctttta cgcaaatatc
    23281 gatcagacgt tgttcaacgt catcgcgatt gctcacttcg cgcagaacca gggtaaaggc
    23341 gttacctttc agcgcgccta aacgcagctt gcgcttgtgc cgcgcatact ccagcacctg
    23401 gcagccttcc agttgaaagg cgctcagatc gggcatttcc ttgcctggca cgcgagcgca
    23461 taaccactgt tccgtaacag catgtttgtc tttttgccca gcgaagctga cttcacgggc
    23521 atgaattttc aggaatttcg ccagtgcatc cgccacaaaa cgggtattgc agccgttttt
    23581 gaggattcta accagaatat gctcaccttc accatcaggc tcaaagccca aatcttccac
    23641 caccacaaag tcttccggat tggctttcag cagcccggtg ccttgcggtt taccgtggag
    23701 gtaagtgaga ttatcaaact caatcatttt gttgccttaa tgagtagcgc caccgcttca
    23761 caggcaatcc cttccccacg tccggtaaat ccaagttttt ccgtagtagt ggctttcacg
    23821 ttaacatcat ccatatggca gccgagatct tcggcaataa atacgcgcat ttgtggaatg
    23881 tgcggcaaca tcttcggtgc ctgagcgatg atagtgacat cgacgttgcc cagggtataa
    23941 cccttcgcct gaatacgacg ccaggcttcg cgtagcagct cgcggctgtc ggcaccttta
    24001 aatgccggat cggtatccgg gaacagcttg ccgatatccc ccagcgccgc cgcgccaagc
    24061 aatgcatcgg tcaacgcatg gagcgccacg tcgccatcag aatgcgccag caatcctttt
    24121 tcgtaaggaa tgcgtacgcc accaatgata attgggcctt caccgccaaa ggcatgtacg
    24181 tcaaaaccgt gtccaattcg cattatgtat tctcctgatg gatggttcgg gtgaggtaaa
    24241 actcggccag tgccaaatct tccgggcgcg tgactttaat gttatccgca cggccttcga
    24301 ccaactgagg atggaatccg caatattcca gcgccgaggc ttcgtcggta atagtcgcgc
    24361 cttcatttag agcgcgcgtc agacagtcat gtaacagctc acgagggaaa aattgcggcg
    24421 tcagcgcgtg ccataagccg ttgcgatcaa cggtatgagc aatggcattt ttgcccggtt
    24481 cggcacgttt catcgtatcg cgcactggtg cggctaggat ccctcccgtg cggctggttt
    24541 cgctcaacgc caacaatcgc gcgaggtcat cctgatgcag acaaggacga gcggcgtcat
    24601 gcaccaatac ccactgcgcg tcgccagcgg ctttcaaacc tgccagcacg gaatcggcac
    24661 gctcatcacc gccatctaca acggtgattt gcggatgatt cgccagagga agttgtgcaa
    24721 aacggctatc gccaggactt atggcaatga cgacacgttt cacccgggga tgcgccagca
    24781 gcgcatgcac cgagtgttca agaatggttt gattaccgat tgagagatat tgcttaggac
    24841 attccgtttg cattcgacgg ccaaatccgg ccgccggaac cacggcgcaa acatccaaat
    24901 gagtggttgc catgttaatt cccgggctga tttatcgatt gttttgcccc gcagactgtg
    24961 cgcgcttcga cgcgtcaggc accagacgat aaaaagtttc gcccggcctg gtcatgctga
    25021 gttcattacg cgcacgctct tcgagcgcct cctggccgcc attgagatcg tcaatttcgg
    25081 caaaaagttg atcgtttcgc gctttaagtt tcgcgtttgt agcttgctgt gccgccacat
    25141 catcattgac gcgggtatag tcatgtatac cgttcttacc gaaccacagc gaatactgta
    25201 gccagaccag aatagccagc aacagcagcg ttagtttacc catcctgccc cctgaaaaac
    25261 ggcatcatca tcccatgcat ccgaagacga ctctacatcc tctgttgggg ataccgcgac
    25321 aacgcgggca aatgtaccac atttgtccat tgttacgtat acccagggcg tgcagaacat
    25381 aatctcatta ttagttacgg tttgaattat gaacagagga gacaagaaag tacaaattag
    25441 cccagtagcc acataaacag tgcgccaaac ataatgccta ctgtcaccag ggtgaaaaca
    25501 atactgtagc gtagctttcc gtccatcaat gaatgcagcg caatccccac cactaccgcg
    25561 acgggcatca gcgccagaaa gaaaggccag gtgtagataa agaagaacag cgtgttagag
    25621 ccataaatca acatcggcat cgccagcgca aataaccagg agataaaacc gaccacggca
    25681 ccaggcagtg accatgtggt ttcttcatcc tcagtaaggc tgtcgttatt tgttagtgta
    25741 atgttatggc tattacgcat atttgatcct gttactttga cgaaccgggc atggaaaccc
    25801 ggtggtgtct caggatctga taatatcgtt ctgtctcaac agatctaata attgctgtac
    25861 caaatttgtt actaattgtt caccattgag atgaatttct gccgattcag gcgcttcgta
    25921 aacggaatct attcccgtaa agttgcgcag ttcaccggca cgcgctttct tatataagcc
    25981 ttttggatcg cgggcttcgc aaatcgccag cggcgtatcg acaaacactt cgataaagcg
    26041 cccttctcct acgcgttcgc gaaccatctg gcgttcggcg cggtgtggcg agataaatgc
    26101 ggtcagcact accagtccgg cttcaaccat caaattcgcc acttcaccga cgcgacggat
    26161 attctcttta cgatcggcat cgctaaaacc gagatcgctg cataatccgt ggcgaacatt
    26221 atcgccatcc agcagatacg tactgacgcc gagtttatgt aacgcctcct ccagcgcccc
    26281 ggcgaccgtt gatttaccgg acccggagag gccggtaaac cacagcacta caccacgatg
    26341 accgtggtgt agctcgcgtt gttgcacagt gaccggatgg ctatgccaga cgacgttttc
    26401 gtcatgcagc gccattattt atcccccagc aaatcgcgcg cgccccagtg cggaaagtgg
    26461 cgacgaacca gagcattcaa ttccagttcg aatgcactga attcagatgg cgcagcagtt
    26521 gcctggctaa ctggctcgtg caccataccg gcacccacgg tcacattgct caggcgatcg
    26581 ataaaaatca gcccacccgt caccggattt tgttgataac gatctaacac cagcggctcg
    26641 tcaaaagtga gatccacgag gccgatccca ttcagtggca ggttttcaac ttcacgctgg
    26701 gtaaggttat taatatcaac ctgatagcga atgccatcaa cacgcgcgcg cgtcttctta
    26761 ccggcaattt tgatgtcgta actctgccct ggagaaagcg gctgttccgc catccatacc
    26821 acatccaccg acgcgctctg caccgccggt aacgcttcgt ctgccgccag cagcagatcg
    26881 ccacggctga tgtcgatctc atccgtcagc accagggtga tcgcttctcc ggcaaaggct
    26941 tcttcgcgat caccatcaaa agtcacgatc cgcgcgacgt ttgattccac accagaaggc
    27001 agcactttta cacgttgccc gacttccacg cgaccggatg ccagcgttcc ggcgtaacca
    27061 cgaaaatcga gattcgggcg attaacgtac tgcaccggga agcgcattgg ctgagcatcc
    27121 accactcgct ggatctccac ggtttccagc acttcgagca gtgtcggacc gctgtaccac
    27181 ggcatacttt cactttgcga agccacgttg tcgccttcca gcgcagagag cggcacaaag
    27241 cgaatatcca gattgccagg taactgttcg gcgaaagtca ggtagtcttt acgaatgcgg
    27301 gtgaatgtct cttcactgta atccaccaga tccattttgt tgatcgctac gaccagatgt
    27361 ttgatcccca gcagcgtgga gataaaactg tgacgacggg tttgatcgag cacgccttta
    27421 cgggcatcga tcagtaagat cgccagctca cacgttgagg cgccggtcgc catattgcgg
    27481 gtatactgct cgtgcccggg ggtgtcggcg ataataaatt tacgcttctc ggtagagaaa
    27541 tagcggtagg ccacatcaat agtgatgccc tgttcgcgct cagcttgcag gccgtccacc
    27601 agcagagcca gatccagttt ttcgccctgg gtgccgtgac gcttactgtc gttatgcagc
    27661 gatgagagct gatcttcgta gatttggcgg gtatcgtgca gcaggcgacc aatcagggta
    27721 cttttgccgt catcgacgct accacaggtc agaaaacgca gcaggctttt atgttgttgc
    27781 gcaatcatcc aggcttcgac gccgccttca ttggcgattt gttgtgcaag tgcggtgttc
    27841 atcttaaaaa tacccctgac gttttttcag ctccatagac cccgcctggt cgcggtcaat
    27901 cacgcggccc tgacgttcac tggtggtgga aaccagcatc tcttcgatga tctccggcag
    27961 tgtttgtgca tttgactcca ccgcaccggt cagcggccag cagcccagcg tacggaaacg
    28021 caccatccgt tttttaatca cttcgcccgg ttgcaggtcg atacggttgt catcaatcat
    28081 catcaacata ccgtcgcgtt ccagaaccgg acgttccgca gcgagataca gcggcacaat
    28141 gtcgatattt tccagccaga tgtattgcca gatatcctgc tcggtccagt tagagagcgg
    28201 gaaaacgcgg atgctttcgc ctttgttaat ctgcccgtta tagttgtgcc acagctccgg
    28261 gcgctgattt ttcgggtccc agcgatggaa gcgatcacgg aaagagtaga tacgctcttt
    28321 agcacgggat ttctcttcgt cgcggcgcgc accaccgaag gcggcatcaa aaccgtattt
    28381 attcagcgcc tgcttcaggc cttcggtctt cataatatcg gtatgtttcg cgctgccgtg
    28441 cacgaatgga ttaatcccca tcgccacgcc ttccgggttt ttatgcacca gcagctcgca
    28501 gccgtaggct ttcgccgtac gatcgcggaa ctcatacatc tcgcggaatt tccagccggt
    28561 atcgacatgc agcaagggga aaggcagcgt acctggataa aacgccttgc gcgccaaatg
    28621 cagcatgacg ctggaatctt taccgataga gtagagcatc accggatttg agaattctgc
    28681 cgccacctcg cgaataatgt ggatgctttc cgcctccagt tgccgcaggt gagtaagtcg
    28741 tttttgatcc ataaccgttc ctttgcaata ccgctatttt cttgccatca gatgtttcga
    28801 ctatagggag cgtaagagaa cgaatgaaat taccaattag aatgagtagt tccttaacgg
    28861 aataacgatt tggcaaagct aatatcaaaa agtgcttaag gcaccggatt tcgggcgttt
    28921 aggaagattt gaaattgttt tagcgcagcg gcagtttcat actatggcgg taaaaaaatt
    28981 tgcatggtat ttaaggactc actatgtttt ccgcattgcg ccaccgtacc gctgccctgg
    29041 cgctcggcgt atgctttatt ctccccgtac acgcctcgtc acctaaacct ggcgattttg
    29101 ctaataccca ggcacgacat attgctactt tctttccggg acgcatgacc ggaactccag
    29161 cagaaatgtt atctgccgat tatattcgcc aacagtttca gcaaatgggt tatcgcagtg
    29221 atattcggac atttaatagt cggtatattt ataccgcccg cgataatcgc aagaactggc
    29281 ataacgtgac aggaagtacg gtgattgccg ctcatgaagg caaagcgccg cagcagatca
    29341 tcattatggc gcatctggat acttacgccc cgctgagcga tgctgacgcc gatgccaatc
    29401 tcggcgggct gacgttacaa ggaatggatg ataacgccgc aggtttaggt gtcatgctgg
    29461 aattggcaga acgcctgaaa aatacgccta ccgagtatgg tattcgattt gtggcgacca
    29521 gcggcgaaga ggaagggaaa ttaggcgctg agaatttact caagcggatg agtgacaccg
    29581 aaaagaaaaa tacgctgctg gtgattaatc tcgataactt aattgttggc gataaattgt
    29641 atttcaacag cggtgtaaaa acccctgaag cagtaaggaa attaacgcgc gacagggcgc
    29701 tggcaattgc gcgcagtcac ggaatagccg caacgaccaa tccgggtttg aataaaaatt
    29761 atccgaaagg cactgggtgt tgtaatgacg cagaaatatt cgacaaagcg ggcattgctg
    29821 tactttcggt ggaagcgact aactggaatc ttgggaataa ggatggttat cagcaacgcg
    29881 caaaaacagc cgcattccct gcgggaaata gctggcatga cgtaagactg gataatcaac
    29941 agcatattga taaggcactt cctggaagaa tagaacgtcg ctgccgtgac gttatgcgga
    30001 taatgctacc gttggtgaag gagctggcga aggcgtcttg atgggtttga aaatgggagc
    30061 tgggagttct accgcagggg cggggaattc taagtgatat ccatcatcgc atccagtgcg
    30121 cccggtttat ccccgctggc gcggggaaca caaaaccaaa cttctccata aattccatag
    30181 ccgcggttta tccccgctgg cgcggggaac acacgcgcgt accggatcgc ggacaacaaa
    30241 ttgccggttt atccccgctg gcgcggggaa cacctgaaac cagttggctc gttgttcttc
    30301 tttggcggtt tatccccgct ggcgcgggga acacggcaac ataacgaaca aaatcaacgt
    30361 caacctcggt ttatccccgc tggcgcgggg aacaccagtt tttgctccca ctgcgactgg
    30421 tggaaaacgg tttatccccg ctggcgcggg gaacacagca ttaatcccca ccagctcgat
    30481 gtgtgtggcg gtttatcccc gctggcgcgg ggaacaccaa accggcctca attgcggccg
    30541 cgcgggccgc ggtttatccc cgctggcgcg gggaacacgg tttcctgatc ctgcgcggtg
    30601 ctgatgggcg cggtttatcc ccgctggcgc ggggaacaca tgaagtttat agcgccaaaa
    30661 ttgacgaaag acggtttatc cccgctggcg cggggaacac tttccgcctg cccacttcaa
    30721 aaacggtctg atcggtttat ccccgctggc gcggggaaca catgcgccac tgcgccgcct
    30781 gcatcgccac cgccggttta tccccgctgg cgcggggaac acactaagca tacatatctg
    30841 tttttaaaca aatttattcc acatcaacaa tctaccaact aaattcaaac atttccttat
    30901 ttttaaagaa cgtctaactt attgattatc aacaggaaga aaagaaacca aacgtaaccc
    30961 atccaaatcc accggaatac gtctgttttt gccccaggtc tgaaactcaa aaccagactc
    31021 agtatttgtc gcccaggcca tgaccacatt tccacaacct gccagttggg taatttgctg
    31081 ccagatcatc tcccgaatac gttttgatgt atcaccaaca tacacaccgg cacgcacttc
    31141 cagtagccag attgcgagcc gtccacgtaa gcgcggcggg acattttctg taacaaccac
    31201 gaccatgctc atcagccgcg tcctcgatga ccgctatcgc ccaacgtttc aggttcgggg
    31261 atggctggcg gtaacatatc cggcgcaggt tgtggcggtt caatttcacc tgcggcaagg
    31321 acttcctcaa ttaacggtat taatttgccc gttaacttag tgctacggaa aatatcgcga
    31381 caggctaatc tgacttcttt atcaggttct gcgggttgcc tcgctgctat ttcaaatgcc
    31441 tttggcacaa ccgaatcaaa tttaatgata tcggctatgt cataaacaaa tgaaagcggt
    31501 ttgccactat ggataaaccc aatagcgggc gcatatcccg cggctaatac tgccgcttca
    31561 gatataccgt acagacatga tgtagcagca ctgatgcagc gattcacaac atcgcctttt
    31621 tcccagtctt taggatcgta tttgcgacca ttccatttca caccatattg tttcgccagt
    31681 aatgcatacg tctggcgaac gcgagatccc tcaattccac gtaactgctc cactgaacgg
    31741 cgagctggcg gtggctcacg aaaacgtaat tcatacattt tgcgcaccac cttcaaccga
    31801 agatcttccg ttaaagcaag ctttgcctgg tagagtaatt tatctgcccg cgcccctccg
    31861 ggttgtccgg atgagtaaac gcgaacgccc gcttcaccga cccagaccag cagtgttccc
    31921 accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg ttcccggttc gagcataatg
    31981 caggcgaccg atcctaccgg aatatgtgtg cggatccctg ttttgtcgat cagcacgaaa
    32041 gcgccgtcca gtacgtcgat ctgaccgtac tggaggaaga tcatggtggt gcgatctttt
    32101 aatgggatcg ggctcagtgg tacaaacgtc acgcctctgc tccgggtttg atcagcatca
    32161 gaccacagcc gaacgcccgg cttttgccat acccctggct cagacgttgc agaaataacc
    32221 caggatcggt gaccgtaagc atccccgtat aatccacgct actgaactgg atcagttgcc
    32281 gggagttttc ccgccgcagt tgctgttgtc ggtaggcatc aaccgaggta tccagcagtg
    32341 taaaaccgct tctctccccc tgcgctgcca gccagtccag cgccgcctgt tgttgatgca
    32401 accagacatc acttccttcc gcctgccccc tcacctgccg tttcgcctcc atcagcagat
    32461 cgtggcgctt gcccgcttta cagattgttg gattcgcccg caggttgaaa caaagggatt
    32521 gtccggtacg caactctggc gcaaatgacc ggcattctat ggtgaacgta tcactttccg
    32581 ccgggcgttc ctgcgacagt acaaaaaagc gaaacgcgcc ctggagttct tcccgacgat
    32641 aaagaaattg cctttctttg ccgccaggga agagatccca cagccactga tgcatcacat
    32701 attcaccgcg atccaccaga tgcagcaact gcgcaggcga aagctggccg gtatgtaagg
    32761 ttattcttga gaggtacatg gttcctcctt gctgagccac ggcccctgat tgacggtgcg
    32821 ttccccaaac agccactgct gacgatttaa aggaacatcc cggcggcgta atattttgct
    32881 cgcgaccagg ccgtcgtgtt tcccttccca ccagcattca tcctgaagtt tcgggagtga
    32941 gactttcagt ttatggaaat gatcctgata ctgttggtat gcattacata aggcatcaca
    33001 cgcgttgcct tcgagcagta acggcgcaag aggtaatgcc agcggatgac tttttcgccc
    33061 cagataaagc ggaaaaactg gatggcgtaa accgtcctgc aactgttcga ggctgtaagg
    33121 cgcatcggct gttgttgcca ccgccaccat ccaccaggca tcggtgtagt agtcgcgccg
    33181 ggagataatc gcgctcaaaa gctcagggtc gctcaactct tcgcgacggc tgaaataacg
    33241 tgctttacgc acctcttttg gcatctggac cgtgtgataa tcccgtgccc agcgcgggtt
    33301 acggctggcg caaaccacca gtgaatagtg gcgattaaac gcgtttaagc gttcggtatc
    33361 atcacgccga atccctaccc cggcagccag tagccccagc aatgctgagc gcgaaggaag
    33421 ttcatgggta tgacgaactt cacctggagc atcgaccccc caggacgcca tcggcccatg
    33481 aagctgaaaa atcagatatt ggctcataag ccgcccctta cgcgcagata aagtccagca
    33541 cgtccttcat gcttccctgt ttgttcatca cgtcaaaact tgcgcattcg gtcttctgtt
    33601 cgtagaccgt attcatgttt tcgcgaagcg ttgtaatacg ctgcaccgcc acatctaact
    33661 gacgtgtgcc attaatgggt tcatagaaag ccgccgccag agagcgtggt tgatcggtgc
    33721 ctttttctgc cagcgcccag gaggcgtagg cacggctggc aaagctgttc tgtttgccgg
    33781 ttggggagac tttaagtgcg gcttccgtaa aggcacgtat ggtctgatta gctaacgctt
    33841 cgtcaccgcc aaggttttcg accagcagat ctttatcaat gcagatatag gtgtagaaca
    33901 gtgcagaacc gaatccggtt tcaccaagat gcccggcacc ggcatcttca gaagcctggc
    33961 gcaaatcatc aacggcggtg aaaaaatcat cttcgacaat cgtttcactg acaccaaatg
    34021 cgtgcgctac ctggcaggcg gcttcaacat taaactcggg tttattcgcc agcatacgac
    34081 caaacatggc aatatctacc gccatacggt ctttgcgtaa caacgcgaga tcttcctctt
    34141 ttggcgcgcg cttttcttct gcaagctgat gggccagcac ttttacggcg tcaaattctg
    34201 ccgggctgat atggactaac tgttcagttt cggcgttagt gagcggatct tttggttttt
    34261 tgtcgttttt agctttccca agataatctg caatttttgc cgcccattcg atggcttttt
    34321 tctcttcgat tcctttctca atcaggatag ttgccgcctc acgcgcaata cgcccactgc
    34381 gaataccaat atggcccgcc agtgcctgtt caaaaagtgc agaagtgcgc cacgcacgtt
    34441 tcagactttg cgaggaaacg cgcagacgcg ttgctccacc cagcaccaca gtttttggcg
    34501 caccggtatc atcacggtta aggttggcag cagggtaagc ggttaacaaa tgaagctgaa
    34561 taaatgttgt catgagatag tcctttattg attttgttcg ttatcggcgt cgccagcctg
    34621 gtaatattcc agcgcccagc ggatacggat aaattcggtt ggccgctgct ggcggcggtg
    34681 atgattgagc aggtcgtcgc tctcctggca ccaacggaag accccctctg ccagagagtc
    34741 aagattgacg gaaccgttta ataatctgac tgcgcggcgt aactggcgca gtaattcatc
    34801 cggtgttttt actgccgaca gacgagtaaa acgccccttt gacattaccg ccgccagctg
    34861 cgcggcaaaa ggctgccgtt catcaatagc tttaacattg gcgctaactg cggctatcag
    34921 cgccagtgcg gtgatacgcc actcaggttc atcctgccac tttatttgtc tgttcttcag
    34981 aaacaggcga aatccatccg tcagacaaac atcattaacc gtcgtactac gccgcagact
    35041 ggcacgttcg ccacgtttct cctgcaattc ctcgtgccat ttgcgcagcg tggctttgtg
    35101 ctcctctttt acaatactca ttcagcagcc tcctgctttt tctccctggc ggctttagca
    35161 ctttgcttct ccgccgatgt tgtaaaatat ttcttgcgcg cggtcatgac gcgttccaga
    35221 tcaacgggct cataaggatt ggtgaataca cgctcgtcaa aatcctgacg tgcgaataac
    35281 caaatttcct tattccattt gctgagtaat tcatccgcat cctgaccttc ttcaatttgg
    35341 cgcactaacc tcagaaagcg atgctgagtt ttgttccaga agtcgatatc cacaaaacta
    35401 aaatcacccc ttgcaccttt tggatcggag aaccatgctt ctttcaatgc actccgtaac
    35461 agacttagaa tccgtgaagc tgtttgcgca gccagccgca gcttcggtat ctgaccttct
    35521 tttttatcga gcagcagcgg gaaatggtgt tcgtaccaac agcgcgcttt catgttgtcg
    35581 aaatcatagc caaatcccca caggcccact tttgcctgct tgagactgct ggcattgaag
    35641 agtttcacca ccagcgcggg aagttccgta ttgttttctg atttacccgt ttcgataagg
    35701 cctaaccagt cacgccagat taaaccgccc ggttgtggtt taacggagta aaactcgcca
    35761 ccctctttaa gtggtacacg gtaaggcgtt aagggatgct gccacatggc ataattcgca
    35821 ccgtaatttt tggtcgtcat caggctcagg agcgcgtcgc tctgctcacc gcaaatatcg
    35881 cagttgccga ctgtcgtggt attaaaatca atacgaatac ggcgaggcat tccccagtac
    35941 gcctggagtt tattgacctg ctcatcggtt accaccgcac cggccagttc gctggtgcgc
    36001 gtcgggccaa gccaggggaa aaccagatcg tcaaattttt tgggtagcgg taaatcggct
    36061 tcatcctgcg gcatgacatt gatccacagt ttgcgccaca agggagtttg ttgattaccc
    36121 tgatattcct gtaattcaat cagagtggtc atcggcccac cgccgcgtaa accggtgcgg
    36181 tagcctttgc cacctgacgg cgcattcaac tgtagggaga acagagctaa cgcagaacaa
    36241 tgtgggcaca ggtattccgt tacaccacgc ttgataaagt ggtctttatt aaacttcgtt
    36301 gtttgagcgc cgggaatctc aggcagtagc gaagcgaccg gaactttatc tccggtgagc
    36361 gcctcgaaat cctgcataaa tgaaggggaa tctggcccaa actggaaagc atgttctaat
    36421 gactgcaatg cttcccgtag cttttcagct tccagtccgt cttcccagat atcatcccaa
    36481 cgacgatgat cttttggcgc gaagctggtt tgtagtagcc ccagcaaaaa ctgccatacc
    36541 gcaccctgga gatctgcccg cggcgcagaa atatcgacaa cattttcatc cgccagatcg
    36601 actggcgcca gtttgcctgt cgttccgtct ttaaaacgaa cgggcaacca cggggttgtc
    36661 agaagtgaaa acgagttcat acgcatcctc tcgtaaaaaa gaccatccca ggtcggttgt
    36721 ccatctttcc atccttttcg gtgtatcaac tattcagaga gtcctgtcgc agacatctca
    36781 accaaccttg ccaatcaatc cttccgtagc cgaatacccg caactttcgt catcgttgac
    36841 taaaatcacg tttgccatct ccggatcttg tcgctgttca atgcaccaca gcctgaacgc
    36901 ctctccctcc agtaaagaaa actcatcccg atgttttttc caccagcttc ggcgcactct
    36961 gacaacgctc atttcccatg cgtgagcacc ggtggcataa ggcttcacca caccgtcaat
    37021 acaggtagcc agccacaggg aaacagactc ctcagccaga cgtgtcgaca gtttttccgg
    37081 gaggtaatcg ttgatattgg cggcatagcc gggcctgaag ttcagcacaa actttttagc
    37141 cattgcgcga tcacagtaat atttgcccac ttgctcctgc tcgctgcggg caaatccttc
    37201 cggcattacc acgtcctcac cgtagacgga ttcaataaga agccgggctg cgtgtggcat
    37261 ctgaatagca ccttgctcac gcagcacacg ctgcgtcagc cagattcgcc catgatcggg
    37321 atagacaaat gcactgttac gcatggcact gccaaaccat tcgtcgccag gagagtcgtc
    37381 ccagacggga gccagaatca gcaattcagg gggggaacgc tcgtcctttc cgtcacgctt
    37441 taactgacca ttaatatcgc ggatatgccg ctgtaatcgt cctgctcgct gaatcagcaa
    37501 atcaacaggg gccaggtcgg agatcatttc gtccaggtca atatccacgc tttgctcaat
    37561 tacttgtgta caaacaatga cttgcgacgc tctctgtaaa ctacaatatt taccaaagcg
    37621 tgccagcgtt tccgtttcaa ttcgctggcg atcgctaaaa gcaaagcggc tatgaaaaag
    37681 tgaaaggctg gaagcaggaa tgacgccgcg ggcaagcagc tgacgatgaa ccttaatagc
    37741 gtcatcgaca gaattccgga tccaggcgat gcatttcccc tgacttaccg ccgattcgat
    37801 acgcgcaata cactcttgtt cactatgaag ccaacccacg ctgacgctac gctcaacgtc
    37861 tttgcgcgtc gctacccggt gtgagttcac atcggatttc gtgacatgcg tcagccaggg
    37921 gtaatcatcc ttttccagga acggagcttc ttgctggccc tctgtacccc gcgcaaaggc
    37981 agcgacgagt ttgtcgcgct gctgttgaga taacgtggca gaaagcaaaa taacgctgtt
    38041 tccgccacgc gcctgccgct cgatcaaccc ttcaagaatg cacgacatgt aagcgtcaca
    38101 ggcgtggatc tcatcagcca gcaggatttt gttactcagc cccagaagcc gcagattatt
    38161 atgtttgaac ggcatcaccg ccatcatcgc ctgatccagc gtgccgacgc caatttcagc
    38221 cagcaacgcc ttcttgttac tgtcggcaaa ccaggccgca catccctgac tgaacgtttg
    38281 ttcatccggt tcttctgacc cgactaaatc accagaccag agtgattcat taaagcggtc
    38341 cattaatgtg cgggcactat gtgccagcac caggctgggg cgggattctg gcgaatagaa
    38401 agcaagccag gttttgacca gccgatcgta catggcattg gccgttgcca ttgttggcag
    38461 accaaaaaac agcccctgag ctttccccgc agctatcaac ctgtgcgcca ggataagcgc
    38521 cgcttctgtt ttacctgcgc cagtcacatc ttccagaata aataactgcg gtccttgctg
    38581 gctgatatcc agatccagca ccttttgctg taacggtgtc ggattctcaa taaaaggaaa
    38641 cagcgtatta attccgttga aaggcgcgac ttttgctttt agaggaaaga cggttaaggc
    38701 gttttgagcc tgaattaaag ttttctgcca gtaaccttta atatccattg ggtgtgcgac
    38761 gcgtggaaaa aatcgcgttg acgaacccgt ccagtctgcg agcacgacgg ttgcagagat
    38821 ataccaggaa aggtgtttta taagttcaat gccctcatca tcatcccaga atgcggggat
    38881 ctctatgagc ggaaacagtg gtttgatttc aaggagaaaa tctcgtgctg cagctttgtc
    38941 ttcaggcaga aaattatcca attcatccat acgatcaggg ggccgaccat gatgccctgt
    39001 cgtcacgggc atccacattt ctatcactcg cctaagtttg cgcggagaga gcgaagatga
    39061 aggaagtaac tcctgacatt cacttaaata atgattccac aaccagtagc ccagcgttga
    39121 atgagagatt ttttcgtaat tttttctggc accttcctga atcttgagtt cagggggcag
    39181 gtaaagttgt tgaaaagagc gggcaaattt tccaatatcg tgccagcaca gcaaccaggc
    39241 gaaaaattga gccgcctgtt ccttgtcaga aatccctaat tgacgaaaat aatcagccag
    39301 cccgaagcaa tttcttttaa ccattaaata gcccatcgcg gctacatcca gcgaatgcca
    39361 gcaaagaagg tgatagtcgt
//
LOCUS       DAZGXZ010000030        38423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000030 DAZGXZ010000000
VERSION     DAZGXZ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.030"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2182
                     /gene="pdeF"
                     /locus_tag="ACHKJF_001694"
     CDS             <1..2182
                     /gene="pdeF"
                     /locus_tag="ACHKJF_001694"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP0949073.1"
                     /translation="PSLILPIFAHINTFAHISSGEVFLFYLPLALMISMMMFFSWAAL
                     PGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGGYRVFTPRRNNVSHGDTRLISQ
                     RIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMPFNLGTLINYQALLVGNLIGVP
                     LCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFALWLLALGALLLLLCMPLNEKST
                     IFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMISIHSYQNYIPIYPGYTTQLTI
                     TSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDPVVHLPNVRALNRALRDAPWSA
                     LCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTES
                     HQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIV
                     TNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILL
                     RMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSP
                     TSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAI
                     DDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAE
                     YVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
     gene            complement(2221..3762)
                     /gene="ppx"
                     /locus_tag="ACHKJF_001695"
     CDS             complement(2221..3762)
                     /gene="ppx"
                     /locus_tag="ACHKJF_001695"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP0949074.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3767..5833)
                     /gene="ppk1"
                     /locus_tag="ACHKJF_001696"
     CDS             complement(3767..5833)
                     /gene="ppk1"
                     /locus_tag="ACHKJF_001696"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP0949075.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6004..6642)
                     /gene="purN"
                     /locus_tag="ACHKJF_001697"
     CDS             complement(6004..6642)
                     /gene="purN"
                     /locus_tag="ACHKJF_001697"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP0949076.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6642..7679)
                     /gene="purM"
                     /locus_tag="ACHKJF_001698"
     CDS             complement(6642..7679)
                     /gene="purM"
                     /locus_tag="ACHKJF_001698"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP0949077.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALAMLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8004..8630
                     /gene="upp"
                     /locus_tag="ACHKJF_001699"
     CDS             8004..8630
                     /gene="upp"
                     /locus_tag="ACHKJF_001699"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP0949078.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8716..10005
                     /gene="uraA"
                     /locus_tag="ACHKJF_001700"
     CDS             8716..10005
                     /gene="uraA"
                     /locus_tag="ACHKJF_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP0949079.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10100..10801
                     /gene="hda"
                     /locus_tag="ACHKJF_001701"
     CDS             10100..10801
                     /gene="hda"
                     /locus_tag="ACHKJF_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP0949080.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(10939..11298)
                     /gene="arsC"
                     /locus_tag="ACHKJF_001702"
     CDS             complement(10939..11298)
                     /gene="arsC"
                     /locus_tag="ACHKJF_001702"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP0949081.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11319..12782)
                     /gene="bepA"
                     /locus_tag="ACHKJF_001703"
     CDS             complement(11319..12782)
                     /gene="bepA"
                     /locus_tag="ACHKJF_001703"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP0949082.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            12995..14056
                     /gene="yfgO"
                     /locus_tag="ACHKJF_001704"
     CDS             12995..14056
                     /gene="yfgO"
                     /locus_tag="ACHKJF_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0949083.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14094..14942)
                     /gene="focB"
                     /locus_tag="ACHKJF_001705"
     CDS             complement(14094..14942)
                     /gene="focB"
                     /locus_tag="ACHKJF_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP0949084.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(14964..16976)
                     /gene="hyfR"
                     /locus_tag="ACHKJF_001706"
     CDS             complement(14964..16976)
                     /gene="hyfR"
                     /locus_tag="ACHKJF_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP0949085.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTAKTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESVLLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17006..17419)
                     /gene="hyfJ"
                     /locus_tag="ACHKJF_001707"
     CDS             complement(17006..17419)
                     /gene="hyfJ"
                     /locus_tag="ACHKJF_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP0949086.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17412..18170)
                     /gene="hyfI"
                     /locus_tag="ACHKJF_001708"
     CDS             complement(17412..18170)
                     /gene="hyfI"
                     /locus_tag="ACHKJF_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFP0949087.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAKRFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(18167..18712)
                     /gene="hyfH"
                     /locus_tag="ACHKJF_001709"
     CDS             complement(18167..18712)
                     /gene="hyfH"
                     /locus_tag="ACHKJF_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP0949088.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18722..20389)
                     /gene="hyfG"
                     /locus_tag="ACHKJF_001710"
     CDS             complement(18722..20389)
                     /gene="hyfG"
                     /locus_tag="ACHKJF_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP0949089.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(20379..21959)
                     /gene="hyfF"
                     /locus_tag="ACHKJF_001711"
     CDS             complement(20379..21959)
                     /gene="hyfF"
                     /locus_tag="ACHKJF_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP0949090.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(21964..22614)
                     /gene="hyfE"
                     /locus_tag="ACHKJF_001712"
     CDS             complement(21964..22614)
                     /gene="hyfE"
                     /locus_tag="ACHKJF_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP0949091.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22626..24063)
                     /gene="hyfD"
                     /locus_tag="ACHKJF_001713"
                     /pseudo
     CDS             complement(22626..24063)
                     /gene="hyfD"
                     /locus_tag="ACHKJF_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24080..25027)
                     /gene="hyfC"
                     /locus_tag="ACHKJF_001714"
     CDS             complement(24080..25027)
                     /gene="hyfC"
                     /locus_tag="ACHKJF_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416978.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP0949092.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTCISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMASLFVALFLSFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFCLAALAWVFWLTGL"
     gene            complement(25038..27056)
                     /gene="hyfB"
                     /locus_tag="ACHKJF_001715"
     CDS             complement(25038..27056)
                     /gene="hyfB"
                     /locus_tag="ACHKJF_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFP0949093.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNTFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIAFVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(27056..27673)
                     /gene="hyfA"
                     /locus_tag="ACHKJF_001716"
     CDS             complement(27056..27673)
                     /gene="hyfA"
                     /locus_tag="ACHKJF_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP0949094.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENAPPQHPLLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(27926..28396)
                     /gene="bcp"
                     /locus_tag="ACHKJF_001717"
     CDS             complement(27926..28396)
                     /gene="bcp"
                     /locus_tag="ACHKJF_001717"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP0949095.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28396..28968)
                     /gene="gcvR"
                     /locus_tag="ACHKJF_001718"
     CDS             complement(28396..28968)
                     /gene="gcvR"
                     /locus_tag="ACHKJF_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP0949096.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDTHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29114..29992
                     /gene="dapA"
                     /locus_tag="ACHKJF_001719"
     CDS             29114..29992
                     /gene="dapA"
                     /locus_tag="ACHKJF_001719"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP0949097.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30009..31043
                     /gene="bamC"
                     /locus_tag="ACHKJF_001720"
     CDS             30009..31043
                     /gene="bamC"
                     /locus_tag="ACHKJF_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP0949098.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31256..31969
                     /gene="purC"
                     /locus_tag="ACHKJF_001721"
     CDS             31256..31969
                     /gene="purC"
                     /locus_tag="ACHKJF_001721"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP0949099.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32123..32350
                     /gene="vapB"
                     /locus_tag="ACHKJF_001722"
     CDS             32123..32350
                     /gene="vapB"
                     /locus_tag="ACHKJF_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFP0949100.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32350..32748
                     /locus_tag="ACHKJF_001723"
     CDS             32350..32748
                     /locus_tag="ACHKJF_001723"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0949101.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32895..33758
                     /gene="ypfJ"
                     /locus_tag="ACHKJF_001724"
     CDS             32895..33758
                     /gene="ypfJ"
                     /locus_tag="ACHKJF_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP0949102.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33773..35788
                     /gene="tmcA"
                     /locus_tag="ACHKJF_001725"
     CDS             33773..35788
                     /gene="tmcA"
                     /locus_tag="ACHKJF_001725"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP0949103.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            35862..36560
                     /gene="ypfH"
                     /locus_tag="ACHKJF_001726"
     CDS             35862..36560
                     /gene="ypfH"
                     /locus_tag="ACHKJF_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0949104.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36641..36841)
                     /gene="ypfN"
                     /locus_tag="ACHKJF_001727"
     CDS             complement(36641..36841)
                     /gene="ypfN"
                     /locus_tag="ACHKJF_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP0949105.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36869..37996)
                     /gene="dapE"
                     /locus_tag="ACHKJF_001728"
     CDS             complement(36869..37996)
                     /gene="dapE"
                     /locus_tag="ACHKJF_001728"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP0949106.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38000..38356)
                     /gene="yffB"
                     /locus_tag="ACHKJF_001729"
     CDS             complement(38000..38356)
                     /gene="yffB"
                     /locus_tag="ACHKJF_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP0949107.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9348 a         9916 c        10122 g         9037 t
ORIGIN      
        1 gccttcttta atcttgccca ttttcgccca cattaatact ttcgcgcata tttcttccgg
       61 tgaggttttt ctcttttatc tgcctttggc actgatgatc agcatgatga tgtttttcag
      121 ctgggcggca ttgccaggga tcaccttagg gatttttgtc cgcaaatatg cagagctggg
      181 tttttacgaa acgctctcat taacggctaa ttttattatc attatcattc tctgttgggg
      241 cggttacagg gtctttactc cccggcgtaa caacgtttca catggtgata cccgtttaat
      301 ttcccagcgt atattctggc agatagtgtt tcctgcaacg ctgtttctga tacttttcca
      361 gtttgctgca tttgtaggat tgctggcgag cagagaaaat ctggtcggtg tcatgccttt
      421 taacctcggg actttaatca attatcaggc cttactggtg ggtaatctga tcggtgtccc
      481 gctgtgctac ttcatcattc gggtagtgcg aaatccattt tatttacgta gctattattc
      541 gcaattaaaa cagcaggttg atgccaaagt caccaaaaaa gagttcgcgc tctggctact
      601 ggcattaggt gctttattgt tgctgttatg catgccgtta aatgaaaaaa gcacaatttt
      661 tagcaccaat tataccttgt cattattgct gcccctgatg atgtggggag cgatgcgcta
      721 tggttataag ctgatttcgc tgctctgggc ggtcgtgttg atgatcagca tccacagcta
      781 tcaaaattac attcccattt atcctggcta taccacgcag ctgaccataa cctcctccag
      841 ttatctggta ttctctttta ttgtcaatta tatggctgta ctggcaactc gtcagcgagc
      901 ggtagtcaga cgcattcagc ggcttgcgta tgtggacccg gtggttcatc tgccaaatgt
      961 tcgcgccctg aatcgcgcgt tgcgtgatgc cccctggtct gcgctttgtt atttacgcat
     1021 ccctggcatg gaaatgctgg ttaagaacta tggcatcatg ctgcggattc aatacaagca
     1081 aaaactttct cactggctgt cacccttgct ggaaccgggt gaagatgttt atcagctttc
     1141 gggtaacgat ctcgcgctgc ggctgaatac agaatcgcac caggagcgca ttaccgcact
     1201 ggatagccat ctcaagcaat ttcgtttctt ttgggatggc atgccgatgc aaccgcagat
     1261 tggcgtcagt tactgctatg tgcgctcgcc agtgaatcat atctacctgc tgctgggaga
     1321 gctaaatacg gtcgccgaac tttccatcgt gaccaacgcc ccggaaaata tgcagcgtcg
     1381 cggggcaatg tatttgcaac gtgaattgaa agataaagtc gcgatgatga atcgactaca
     1441 gcaggcgctg gaacacaacc attttttcct gatggcccag ccgattaccg gtatgcgtgg
     1501 tgatgtttac catgaaattc ttctgcgcat gaaaggtgag aatgatgaac tgatcggccc
     1561 cgatagcttc ttgccggtcg cgcacgaatt tggtttatcg tcgagtatcg acatgtgggt
     1621 cattgagcat acgctgcaat ttatggctga aaacagagcg aagatgcccg ctcaccgttt
     1681 tgctattaat ctgtctccaa cctcggtatg tcaggctcgt tttcctgttg aagtcagtca
     1741 gttgctggat aaatatcaga ttgaagcgtg gcaacttatt tttgaagtca ccgaaagtaa
     1801 tgctctgacc aatgttaagc aggcgcaaat caccttgcag catcttcagg aattaggctg
     1861 ccagattgcg attgatgatt tcggcaccgg ctacgccagc tatgcgcggc ttaaaaatgt
     1921 gaatgccgat ctgcttaaaa ttgacggcag ttttatccgc aatattgtgt caaatagtct
     1981 ggattatcag atagtggcat cgatttgcca cctggcgcga atgaagaaaa tgcgggtagt
     2041 ggcagagtac gttgaaaacg aagagatccg cgaggcggtg ctctctttgg ggatcgatta
     2101 tatgcagggt tatcttattg gtaagccgca accgttaatt gatacgctga atgaaatcga
     2161 acccattcgc gaaagtgcct gaataatgcg ggccgacatt tctcgtcggc ccgcaaagca
     2221 ttaagcggcg atttcaggtg tactttcttc ttcaattttc aaccgccagc cagccacgcc
     2281 ttcccagtat tcttgctcct tttccagatc aagcagtacc agcgcattct gactaaacca
     2341 gtcatgcggg aaacgcagtg tccagtgact gtcatcagta atcagtgtca atgttggcgg
     2401 tgtggtggtt gcctgacgtt gattgtttag taatacgcca aggcgcaata gctgtatcag
     2461 tggcaggaac tgtttcttct taaacaaggt aaagcgcggc agatcgtcga gcttaatcgc
     2521 tttacggtga tagcgcacca gtgtcgccat catcagctgc tgttcctgat taaaacccgg
     2581 caagtcactg ttttgcagaa tataagcgga gtggcgatgc aaaccgctgt ggttgatatt
     2641 caacccgacc tcatgcagca tggcggccca tcgcagtagc gcctccagtt gcggatgcgc
     2701 cagcttcggt tgctgttccc gccactgttc gtacatttgc atagtggtat ccagcacccg
     2761 tcgggcctgc tcgctgtcga tgtgatactg gttggcgagg ctgctggcgg tgcgactacg
     2821 cacatcctga tgacggaaac gtccttccat ttcatacagt acgccttcgc gtaacgcccc
     2881 gtcagaaagg cgcagttcac ggatggctaa agcatcaaac acaccgcata aaatcgccag
     2941 tccaggaacg aagactgttt tccgctcttc ggaaagaccc ggtaaactca gcgatgcgaa
     3001 attacggtga cgtaaaactt cttttaccag tttttccaga cgttccgggg taattatccc
     3061 gtctttctcg cccatttcca ttaacacttc atgggcggct tttatggtac cggaagcgcc
     3121 cattgccacg ttccagccct gaatacggaa ttgccaggtt aaagtttcca gtttttgtgc
     3181 tgccgccata cgagcgcgct gaaaattctc tttattgatg accccgccag ggaagtaaag
     3241 ctgggcaaag ctgacacaac ccatccggcg gctttcaacg agaatcggtt cgaaattttc
     3301 accaatcacc agttccgtag atccgccgcc aatatcaata accagtttgc gacctttctc
     3361 cggttgggta tgttccacgc ccataaaaat cagacgggct tcttcattac cggaaataat
     3421 ttcaatcggg tagggaatga ccttttccgc gcgtttcaga aagtcagtgg cgttcagcgc
     3481 ctgacgcagc gtatgggtgc cgactataca gacgctggca ggagaaaacc cttgtagccg
     3541 ttcggcaaac agcgacagac agttcaaacc gcgcgtcatt gcctcttcac tcaacatatt
     3601 atctggcccc aggccgtccg ccagatgcac ccgctgtttc aggcggccaa taatctgcat
     3661 ggcaccatct accacacggg ctatgaccat gtgaaaactg tttgaaccaa gatcgaccgc
     3721 agcaaactcc tgcggacgag gggatttatc gtgtattggc atagggttat tcaggttgtt
     3781 cgagtgattt gatgtagtca taaatcgcca actgcgcccg tactttgcgg cgattgccgc
     3841 ggggaacgta gcgattactg agttctttat cgatataacg agctttgacc gtatcgctga
     3901 acaatatgtc gatgatgtcc agcacccgct gcttcaggcg cggatcgagc agcggtgtcg
     3961 ccacttcaat acgataatca atattgcgcg tcatccagtc ggcggaagaa aggtagacct
     4021 ttttatcgcc gccattttca aaaatataaa cccggtcatg ttcaaggtaa cggtcaacaa
     4081 tactgatggc tcgaatgttg tcgctaatgc cttccagatt ggggatcagc gaacacattc
     4141 cgcgaaccag cagattaacc ggtacgccgg agctggaggc cgcatacaga cgatcaacca
     4201 ggcctttatc gacaaggtta tttagcttca gggtgatacc actgggcagc ccttgctgcg
     4261 cgttggcgat ttcgcggtct accatttcat ataacagacg gcgagagttt tgcggtgaca
     4321 ccattaaata atcaaatgtc accgggcggt atgggttttc aataaagtta aatacccgcc
     4381 gtacttcgtt ggtgatacgc gcatctgcgg tcagcaacga atagtcagta taaagacgcg
     4441 cggttttttc gttaaagttc ccggtcccga tgtgtgcgta acggaccact tcaccgtttt
     4501 ctttacgtga aatcaggaac agtttggcgt gaattttcag ccccggcgca gagaagataa
     4561 cgtgcacgcc tgcttcggtc aggcgcttcg cccagtgaat gttggcttct tcgtcgaaac
     4621 gcgcctgtaa ctcaaccacc acagtgactt tcttaccgtt atgtgcggcg tggatcatcg
     4681 agtcgatgat gcgtgaatct ttcgccacgc ggtaaatgtt aattttaatc gccagtacgc
     4741 tcgggtcgaa cgaagcctga cgcagcagtt ccagcacatg ctcaaaggtg tgataaggat
     4801 aatagagcaa cacatcgcgt tcgcgaatgg catcaaaacc attgcggaac tgggctttat
     4861 caaaccaaat gtggcgtaaa cgcggcagtg gtttgttcac cagattggct ttgccgacat
     4921 tggggaaatt aataaagtct ttaaaattat gataacgacc gccggggacg atggagtcgt
     4981 agcgggaaat agtcagtttt tcgcgtaaca cttcaaccag cgcattgggc atatcgcgct
     5041 gataaacaaa acgcaccggc tcagcagtta aacgctgctt gagactggaa gacatcaact
     5101 ccatcaggct ggcttccatc tcatgcacta aatcgtattc ggcatcgcgg gtcatcttca
     5161 ttgaataggc attcagcgcg tcataatcaa agaagccttt gaaaatatca tcaaggcagt
     5221 aacgcagaat gttatccaga agaatcatcg gcttgcgtcg acgcggtgct tctggcggta
     5281 aattcacaaa gcgcggcact ttatctgatg ggatctccag cagcgcgtaa cggatggtat
     5341 cgccacggat aatttccacc gccagatagg tgtaatcatc tttcaggaac tgcactaagt
     5401 cagtgtcagg attgattaaa atcggcgtaa tgtgctgacg cagatactgc ttaaaataat
     5461 gacgaagcca gttttgttga ttgacggaga gctggcgttc attaatcagg aagatctggt
     5521 tacgcgccat ctccagcagc agctcgttgt agaggccgtc gaattcctga tcggctttca
     5581 gcacccggga ctgaattttg cccagtaaat ggcgggaatg agagttggag ccttgttctt
     5641 cgctaataat gatgcgtcgc ttcagttcag cgaagcggac tttatagaac tcatcaaggt
     5701 tattggaata gatccccagg aaacgcatcc tttcaatcag cgggttagat ttgtccgccg
     5761 cttcctgaag cacgcgttca ttgaacgata accaactgag ctctttttcg atgtatagct
     5821 tttcctgacc cattaccact tttactccgt tttattcacg ggacactgcc tggatattat
     5881 ggcgagcaat attggcagat gtccaactat gccagaaaag ggggaaaaaa actgctggag
     5941 cttgcgattt acaggtgaat tgaaaaacgc cagcggcaga gctggcgctt taactacggg
     6001 gcattactcg tcggcagcgt agccctgcgg cggcagacgt tgaccatcca gccacgcggc
     6061 gttttcgtgc attttcagac gaccatcggc aaaccagcta atcaccagtg gataaatagc
     6121 gtgttcctgg gtttgcacgc gggcggtgat gtcatcttcc gtatcaccag caaataccgg
     6181 gactttcgcc tgtaaaataa ccgggccacc gtccagttca tcggtgacga aatgcaccga
     6241 tgtaccgtgc tcttcatcac cattttccag cgcctgacga tgggtgtgta atccgggata
     6301 tttcggcagc agagaagggt gaatgttcag caaacgcccg gcatagtggg agacaaacgc
     6361 cgggctgaga atgcgcataa aaccagccag cacgaccaca tcgggtgcgt acatgtcgat
     6421 ttcatgaatc aactcccggt cataggcttc acgactgtca aacgcgctgg cgatgagcgt
     6481 atgcgttgca atacccgcct ggcgggcgcg ttcaaggccg aacgcgtcgg ccttattgct
     6541 gaaaactgcc cgtacggtgc ctttaatttt gttagtttta caggcgtcaa taattgcctg
     6601 taaattactt ccgttgccgg aaataagcac cacaatattc attattcgat aaccacgcgt
     6661 tgttcggaat cagaggcttt gatgataccg attttccacg cgttttcacc gttggagttt
     6721 agcatggcga gggctttgtc cacttccgga gcaggcaggg cgataatcat cccgacgccg
     6781 cagttgaagg tgcgatacat ttcatggcgc tcaacgttac ctgccgtttg cagccagttg
     6841 aacacttccg gccactgcca ggaagattca tcaatcactg cctgagtatt atctggcaat
     6901 acgcgtggaa tgttttccca gaagccgccg ccggtcaggt gcgcaatggc atgcacatcg
     6961 accttttcaa tcaactccag cactgacttc acgtaaatgc gggtcggtgc cagcagatga
     7021 tcggctaatg gcttaccatc aagttcggtg gtttgcggat cacaaccgct gacttcaaga
     7081 attttgcgca ccagcgaata gccgttcgag tgcggaccgc tggaaccgag tgcaatcagc
     7141 acatcgccgt cgctgacttt agagccgtcg atgatttctg atttttctac cacgccaacg
     7201 cagaaacccg cgacatcgta atcctcaccg tgatacatcc ccggcatttc tgccgtttcg
     7261 ccacccacca gtgaacagcc cgattgcaga catccttccg cgataccgct gatcaccgct
     7321 gaagcggtat caacatccag ttttccggtt gcgtaatagt cgaggaaaaa cagcggctct
     7381 gcaccttgca ccaccaggtc attaacgcac atggcgacca gatcaatacc aatggtgtcg
     7441 tgacgtttta agtccatcgc cagacgcagc ttggtaccta cgccgtcagt accggaaacc
     7501 agcacaggtt cacgatattt ttgcggcaat gcacacagcg caccgaagcc gcccagaccg
     7561 cccatcactt ccggacgacg cgttttcttc actacgcctt tgattcttcc aaccagagca
     7621 ttacccgcgt caatatcaac accggcatct ttgtagctaa gagaggtttt atcggtcact
     7681 gcttgggtcc ccacgcgtta cttgcggtag aaaaataaaa ttcggcgcaa ttctaacagg
     7741 gaaagcaaac gtttgcgaga ctgctttaca caaccttttt gcacgtcttt tgcccaggca
     7801 cgcggcgaaa gaagacttgt gccagggtaa aggttagttt tcggatggaa taatcttctt
     7861 tcataaccat ttgaatataa aataacttta tctcaaaccg ttatcatttt gactaaagtc
     7921 aacgaaaaga atattgccgc cttgaagaaa ggaggtataa tccgtcgatt ttttttgtgg
     7981 ctgcccctca aaggagaaag agtatgaaga tcgtggaagt caaacaccca ctcgtcaaac
     8041 acaagctggg actgatgcgt gagcaagata tcagcaccaa gcgctttcgc gaactcgctt
     8101 ccgaagtggg tagcctgctg acttacgaag cgaccgccga cctcgaaacg gaaaaagtaa
     8161 ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat caaaggtaag aaaattaccg
     8221 ttgtgccaat tctgcgtgcg ggtcttggta tgatggacgg tgtgctggaa aacgttccga
     8281 gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga aacgctggag ccggtaccgt
     8341 acttccagaa actggtttct aacatcgatg agcgtatggc gctgatcgtt gacccaatgc
     8401 tggcaaccgg tggttccgtt atcgcgacca tcgacctgct gaaaaaagcg ggctgcagca
     8461 gcatcaaagt tctggtgctg gtcgctgcgc cagaaggtat cgctgcgctg gaaaaagcgc
     8521 acccggacgt cgaactgtat accgcatcga ttgaccaggg actgaacgag cacggataca
     8581 ttattccggg cctcggcgat gccggtgaca aaatctttgg tacgaaataa agaataaaaa
     8641 taattaaagc cgactttaag agtcggcttt tttttgagta aagcgcctat aacacataat
     8701 acagaggata atactatgac gcgccgtgct atcggggtga gtgaaagacc gccactttta
     8761 cagacaatcc cgcttagttt gcaacatttg ttcgccatgt ttggtgcaac cgtcctggtg
     8821 cccgtcttat ttcatattaa cccggcgact gtactgttat ttaacggtat tggaacgctg
     8881 ctgtatctct tcatctgtaa agggaaaatt ccggcttatc ttggttccag ctttgccttt
     8941 atttcaccgg tattgttact gttgccgttg gggtatgaag tcgcgctggg cggctttatt
     9001 atgtgcggcg tgctgttctg cctggtttct tttatcgtga agaaagcggg gaccggctgg
     9061 ctggacgtgc tgtttccacc tgcggcaatg ggcgcaatcg ttgccgtcat cggtctggag
     9121 ctggcgggcg tagctgccgg tatggcgggt ttactcccgg ctgaagggca aacgccagac
     9181 tccaaaacca tcatcatctc tattaccacc ctggcggtca cggttttagg ttccgtgctg
     9241 ttccgtggtt tcctggcaat tatcccgatt ttaattggcg tgctggtggg gtacgcgctc
     9301 tctttcgcaa tgggaattgt cgataccacg ccgattatta atgctcactg gtttgcgctg
     9361 ccaaccctct atacgccgcg cttcgagtgg tttgccattc tgactattct gccagcggca
     9421 ttagtggtta ttgccgaaca cgtagggcac ctggtagtaa cggctaatat cgtcaaaaaa
     9481 gatctgctgc gcgatccagg tctgcaccgt tcgatgtttg ctaatggctt gtcgaccgtg
     9541 atctccggct tctttggctc tacgccaaat accacttacg gagaaaacat tggcgtgatg
     9601 gcgatcaccc gtgtttacag tacctgggtt atcggcgggg cggcgatttt cgctatcctg
     9661 ctttcctgcg tcggtaaact ggctgccgct atccagatga tcccattgcc ggtgatgggc
     9721 ggcgtttcgc tgctgcttta tggtgtcatc ggtgcttccg gtattcgtgt tttgatcgaa
     9781 tcgaaagtgg attacaacaa agcacagaac ctgatcctga cttccgtgat tttgatcatc
     9841 ggcgtcagtg gcgcgaaggt aaacatcggc gcggcggagt taaaaggtat ggcgctggcg
     9901 accatcgtcg gtatcggctt aagtttgatt ttcaaactga tcagtgtgct gcgccctgaa
     9961 gaagtggtgc tggacgcaga agacgcggat ataacagaca aataatgtac cgaccgggca
    10021 gtgttgctgc ccggttcaaa catcatggga ttctgtggta aacttctcgc gattttgtga
    10081 aatcctggtt gaggtatctc tgaacacacc ggcacagctc tctttgccac tttatcttcc
    10141 tgacgacgaa acctttgcaa gtttctggcc gggggataac tcctctttac tggccgcgct
    10201 gcaaaacgtg ctgcgtcagg aacatagcgg ttacatctat ctctgggcac gcgaaggcgc
    10261 ggggcgcagc catctgctgc acgcggcttg cgcggaattg tcgcagcgtg gcgatgcggt
    10321 gggctatgtc ccgctggata aacgcacctg gtttgttccg gaagtgctcg acggtatgga
    10381 gcatttgtcg ctggtctgta tcgacaacat tgagtgtatt gcaggcgatg agttgtggga
    10441 gatggcgatt ttcgatctct acaatcgaat tctggaatcg ggcaaaacac ggttgttgat
    10501 caccggcgat cgtccaccgc ggcagttgaa tctgggatta ccggatctcg cgtcgcgact
    10561 cgactggggg cagatctaca aattgcagcc actttctgat gaagataagt tgcaggcgct
    10621 acagttacgc gcgcgtttgc gtggttttga actgccggaa gatgtggggc gtttcttgct
    10681 gaagcggctc gacagagaaa tgcgcacgct atttatgacg ttggatcagt tggatcgtgc
    10741 gtcgattacc gcgcaacgta agttgaccat tccgtttgtg aaagaaattc tgaagttgta
    10801 gataaagtgt ttattgtcgg atgcggcgcg aacgccttat ccgaactacg cgaagttaat
    10861 ccatagtatt cgtaggccgg ataagacgtg gcaagcgtcg catccggcaa tgtgcttcaa
    10921 ctagcgatgg agcacatctt aacccacgat ttccagtacc tgctccggtg gacgaccaat
    10981 ccgcgctttg ccattcgcga ccacaatcgg gcgctccatc agcttcggat tatccaccat
    11041 tgcctgaatc agcgcctctt cgctaagcga actgtcagcc aggttcagtt ctttataaag
    11101 atcctctttc tggcgcatca attcacgggc gctattcatc cccagcattt tcagcaaatc
    11161 gcgcagcgtt gccgcatcgg cgggtgtctc aaggtagagc accacttctg gctccacgcc
    11221 gttttctttc agcaggttca gcgtttcccg gctctttgaa cagcgtgggt tatggtagat
    11281 ttttacctgt ttggtcattg ttcttccttt aatgcgaatt acatcttggt ataaggctta
    11341 aagcgttcct gcagctggcg caactggtcg atgcgcgcat cgtaacgcgc ttgttgcagg
    11401 ctgcctaatt tcacctgcga actggcgcta ctcaacagcg aaatggcctg atcgagtcgt
    11461 ccggcgagcg cataaccttc tgctcgcgca gccagctcct gatcgcggtt atttagcgcg
    11521 gcttccgcct gtgccagcaa atcccagccg ttgctgtcat ctttattatt aaaggtgtag
    11581 cgattgagaa tattggccgc ttcttgtggt tgaccgcctt gcagataagc gttcgccagg
    11641 ttgagctgca acaccggatt ggtgcgtaaa tcgcgggcat ttttcagacg attgatcgcc
    11701 tcattggctt tgttttgccc aagatcgata tcagtagcca gatcgagata ccatgcgttg
    11761 ccaggttctg ccgccagtaa cggttgcagc gttttacgcg cctcgtcgta tttattggct
    11821 tccatcgcct gtaaagcacg accatattgc gccgctcgtt gctgacgaac gtttcctttc
    11881 gcccattcat ccagcaaatc actggtgagc tggttacgtc cggaattata catccccagt
    11941 gtgcgcgctt tcgccagata gaaatcttcc gacgactgca ccaccatcgg tcgcatctga
    12001 ttagcacggt tgcgggcatc tgccagacga ctttccggca acgggtgagt caataaaatt
    12061 tccggcgggc gcgaggagta acgcgcctga tcgagtaatt tttcgaggaa ggttggcatc
    12121 gcctgcggat cgaatcccga gcgttgcagc acctgaatac caatgcggtc cgcttcctgt
    12181 tcattttgct gggtgaaact gatcatcccc tgacgtgttc ccgccagtgt accggtcagc
    12241 gccgccatcc ccgcctgcgg actggccatc gccagtaaaa tagaacctaa cgcgccgacc
    12301 caggtcagcg gcgcgctgcg ttgttgatct tccatcgctc gcgccaggtg acgttgggtg
    12361 acgtgggaga tttcgtgcgc cataactgaa gccagttgac tttcgttatc ggaataacgg
    12421 aacagggcag agtgcagcac cacgttgccg ccaaagaaag caaaggcgtt aatttcgtcg
    12481 ttgttgatca gaaaaaaatg aaacggtgtc ttaaccgaat tggcatgcga aaccagacgc
    12541 atccccagcg aattaatata ttgcgttaac agcgggtcat taattaacgg cgcgctgccg
    12601 cgtagctggc ggacataata gtcgcccatc tgcatttcct gaccaatgga aagcgtgctt
    12661 cctgcggagg ttcccatatc cggcaaggtg tctgcgctgt cggcaaacgc cggggctacc
    12721 tgaccaatgg tcatagcagc aatgagggtt gcaaccaggt tttttttcaa ctgcctgaac
    12781 ataacctcta tcctgtattt ctgaagatag tcatttgacc gatactgtta aatatcgttc
    12841 ccaccgcgcc gcctttgagt gtaacggcga agattagccc actcaaatga cttttcagtt
    12901 tcctgaatcc gtcataaaaa gcgaagtctt ttttgtgaca tttcgataca attgcggatc
    12961 gcaatcccgc tcttgaggtt cagggaaggt ctttatgctc gaaatgttga tgcaatggta
    13021 tcgccgccgt tttagcgacc cggaagcgat tgccttgctg gttattttag ttgccggatt
    13081 tggcattatc tttttcttta gtggcctgct tgctccgttg ctggtggcta ttgtgctggc
    13141 ctatttgctg gaatggccaa ccgtgcgcct gcaatctatt ggctgctccc gccgctgggc
    13201 gacgtcgatt gtattggtgg tttttgtcgg tatattgcta ctgatggcgt tcgtggtact
    13261 gcctatcgcc tggcaacagg gcatctactt aatccgcgat atgccgggga tgctcaataa
    13321 gctttctgac tttgccgcca cgttgccgcg ccgctatccg gcgttaatgg acgcgggcat
    13381 tattgatgca atggccgaaa atatgcgcag tcggatgctg accatgggcg attcggtggt
    13441 gaaaatttcc ctcgcctcgc tggtcggttt gctgaccatt gccgtctatc tggtgctggt
    13501 gccattgatg gtcttcttcc tgctgaaaga caaagagcag atgctgaacg ccgttcgccg
    13561 ggtgctgccg cgtaaccgtg ggctggcagg acaggtgtgg aaggagatga atcaacaaat
    13621 caccaactat atccgcggca aagtgctgga gatgatcgtg gtagggatcg ccacctggct
    13681 ggggttcttg ctcttcgggc tgaactattc gctgctgctg gcggtgctgg tcggcttctc
    13741 ggttcttatt ccgtacattg gcgcatttgt ggtgaccatt ccggtggttg gcgtggcgct
    13801 attccagttt ggcgcaggca cggaattctg gagctgcttc gcggtgtatc tgattattca
    13861 ggcgctggac ggcaacctgc tagtaccggt gttgttctcc gaagcggtta acctgcatcc
    13921 gctggtgatt attttatcgg tggtgatctt cggtggtttg tggggattct ggggcgtatt
    13981 cttcgccatt ccattggcga cgctgatcaa agccgtgatc cacgcctggc ccgatgggca
    14041 aatcgcgcaa gaataatctt cagcccgacg aactgtttcg ccgggctgat ttttcaggat
    14101 tcctgacgta aataaatagc ccgataacaa atactcacca gcactgcacc gccgataata
    14161 ttacccagca tcaccgggag cagattggcg gtaataaaat ggctgaccgt cagtgccgga
    14221 aaattgtctg cgctactgtg cgccagttgc cagaaggagg taggggcgaa atggcgaatg
    14281 gcaatggcga acggaatcac aaacaaattg gcgatacagt gctcaaagcc actggcgaca
    14341 aacagggtga tgggcaaaat catggcgacg attttgtcgc ataacgaacg cccgcaataa
    14401 ctcatccaca gcgccaggca aaccatcaga ttgcacataa tgccgaggct gacagattca
    14461 gtaaatgtat gatgcatttt gccctcggcg cagtgtaaaa ccgcgactcc ccacatcgcg
    14521 ttttcactca tcaccagccc ggaaaaccag attaacaaac tgaaacaggc aatacctgcc
    14581 agattaccgc aggccaccag aagtgcgtta atcagccaag ttcgccaact aataacgccc
    14641 cgactttttg ccattaccgt cattaccgac gaggtgaaca ggttggtgcc gcaaaccgcc
    14701 agcaaaataa agccaagtgt aaagcaaagg ccgcccacca gatgggttaa tgcctgtgaa
    14761 gacggggcat cggcgataac tgaaaggtaa aaagtaaatc cgatcgccat aaacacgcca
    14821 gcggacatcg ccattaaaaa gaccgacact ttactgcggg caattttatg ggcagcaatc
    14881 cgttcagcga ttgccgcttt tctggcgctc aactgcaagt cgaaagagag tttgtttctc
    14941 atgcatactc ctgcaaaagc agattacaac acctcgcgaa ccgagattcc cagacgctgc
    15001 attcgtgaca gcagcgtggt gcgcttcatc cctaatcgtg tcgccgcgcc acggggcccg
    15061 gcaacaatgc cattggtttc tcgcaatacc tgaacaatgc gctgacgttc ttcttcgtca
    15121 ttctctggcg tcgtcgggtg cagcaactgc gccattgaac tgcgaagcgc tgaatcttca
    15181 tttagcgtcg gcagtaaacg gctttgtcgg acattaagat gtaaattcag actgttaccg
    15241 cgagtcagca gtaccgctcg ctcaatcacg ttttccagct cgcgcacgtt gcccggccag
    15301 tcccacgaca tcaactggcg tagcgcctcg gtcgggatgg cgtcaattga acgattcata
    15361 tggcgcgcca ttttttgcgt gaaatgcttt gctaaaagag ggatatcttc cggacggtcg
    15421 cgcagcggcg gcaattccag tgggaagaca ttcaggcgat aaaagagatc gctgcgaaac
    15481 tggcgatctt caaccatttg ccacaaatca cggttggtgg cggcaatgac tctgacattt
    15541 accgggatcg ttctactccc gccgagacgc tcaatctccc gttcctgcaa tacgcgcagc
    15601 agtttaggct gaagttctaa cggcagatcg ccaatttcat cgagaaacaa cgtgccgcca
    15661 tcggcaattt caaaacgacc acgatgggta ttaatcgcac cagtaaacgc ccctttgtca
    15721 tgaccgaata actcactttc cagaagactg gcggggatgg cagcgcagtt gattttgacc
    15781 agcggcttgt cgcgtcgcgg gctaagttga tggatcgctc tggcgatcac ctctttgccg
    15841 gttccggttt caccgcaaat caacaccgta ctgtcgctct tcgccacaat atctacctgc
    15901 tggagcaggt cttccattgc ctggctttga tagataatgt caccgatgcc cagattcgaa
    15961 aggagctgct cgctaagctg gtggttttct tgctgcaaac tttcctgcaa atcggtcatg
    16021 ctacgccagg catcggcatt gccaacggca atagcgatgc gatcggctat gtgttgtagt
    16081 agctggcagt tttcctcact aaagagagtg gaagaggtat gcgccagcaa caatgcaccc
    16141 ggtgtatggt tgccaaaggt aagcggtata aggagcacag attcgcagcc gttagatgca
    16201 agtaagagta ataacgggtc gcgttgccag agaaacagat cgtctgcacg gtgcgtcaag
    16261 gtcggttgtc gggtttgtag cgtttgtgtc agcaatacac tgtcgccagg catattgcgt
    16321 ggcagacacg cacaatgtga ggcagaaaga tcgctgcacc acaggctaaa cttctcgttc
    16381 tttcgatgat cgcccagtac catactgact gaagccagac cgaaaaaatg atggatctca
    16441 cgagcgacgt cagcgatcag atcgtcgaga tcaagatgtg acagcactgc attagtaata
    16501 tcaaccagta cccgatgatg atcgcgatct ttctccagca aacgcagcgc cacctgctgt
    16561 tcttcatcat tgagccatga ttgcagaacc ggggtgatta tccgcgccag ccagttaaac
    16621 agtgtgcaat cctggggagt aaaacggctg ccgtgggtgc gaataaagcg tagctccgcc
    16681 attccactac tgccgagaag gacggcgtga cgcgaataat cgaccatctc gccctgatac
    16741 tctgtttttg ccgtcacatc gctgccatca tcatggtagc ggtaatagaa gttgcttttg
    16801 agaacatgca gttcaataag ttgtaccggg ctgaacggcg gcggcatcgg gatgaaggcg
    16861 cgtaataaag acgccttacc gtgtttctgt gctaaccgct ccgcgagcat tccgtttacc
    16921 gcttcaattg ttattccttg tggcggggca aacatcgcct cgtctgacat agccatcaac
    16981 agtccttatt aatgagaaat ccttctcacg caatttttct ggccatcaga tagatggccg
    17041 gttcctgcac aatttcatcc aacagatgaa tgaattgaat gctacgcgcc tgccacacag
    17101 acgtcgcgtc atccgccagt ggcgcgaatg cccgggtcaa cagggcggtg tggctggcgt
    17161 caataacaat ctcaccaaaa gtcatcaccc ccagcatctt acgtcgggct tgctcctctt
    17221 cgacctgggc aagccaatct tcgtattccg tcagtgggca ggggaaggcg acattcagac
    17281 aatcaatcac gccaacgtga tgaccgatag ccagcgagta atacattacc tgagagctgt
    17341 gttccggcat ctcgtcgcta ctggcgacaa acttttttcg cagcgtccag aaaacaattt
    17401 ccccgcactc ttcagtcatg taacccccgg agtacgcgaa agagttgctg aacgatacta
    17461 ttgagacgtg gatcgtcggc atcgcgcaac caggtgttaa cccgatttcc tgtaggatcg
    17521 tcgcttaaat ggcgcaggag ccgatcgcaa atttctcgcc cctgacgata gcccgccagc
    17581 cgccgcgctt ctcgctcaat ggcgatacgc tgtgatggcg ggatctgtgg ccacaacggt
    17641 tgcatagtca ccccggtggg atcgcgataa tccacagcgt gaatcttctg ttgcagcaaa
    17701 ccgagcgcca cggcgaaacc gtgaatggtg gcggccggtg ttggcgggca gccggggatc
    17761 caaacatcaa tggggacaat ggtgtcgctg ccgccccaga cgctgtagag atcgtggaaa
    17821 ataccgccgc cgacaccgca cgcgccgtag gaaacacaga ttttatgatc gggggcagac
    17881 tcatacgccc gaagtgcagg catacgcatc gcccgggtga ctgcgcctgt aaataacaaa
    17941 atatcggcgt gacgcggtga tgaaataacc ttaatgccaa aacgttttgc gtcgaatact
    18001 ggtgtgatgg cggcaaaaat ttcgatttca caggcgttgc agccgccgca atcgacgcga
    18061 taaacgtaag ccgagcgacg gatatcctgt agcaaatgct gcttcatctt ttgcgtttgc
    18121 tcgtccagcg tgatgggctg gctgacatgt tgtgtaagca ctggactcat agctgctcct
    18181 tagccaccag cggtacatct gtatcgtcgt tgagcagcgt cgcgcgttgt ttgcactccg
    18241 ggcagacgct cgcttgcgcc cataacattt cgcggttttg tggcgcattt tgttgctgtg
    18301 ccaacaattc agcagccagt gcgacggttt tttgcggggc aaacgggcgt tcgcaacggc
    18361 tgcaacgttg tagatggaac gtcgcgcggg tatagagatc ggctttattg gtgacggtca
    18421 gttcaaagtt attggtaagc tggatggctc tggtcgggca cacttcttca caacgtccgc
    18481 agtaaataca acgcctcaga tagagctgcc aggtgcgcga attttgctgg tcgtcggtct
    18541 ggatagtcag cgcatttgcc ggacaagcac aggcgcaggc accgcaggca atacattggc
    18601 tgggcatcag gtccggtttc ccgcgaaagc ctgggctgac ctccagtggc gcgaagggat
    18661 atttcaccgt cgcggttccg gcgcgcataa tagttttcag taacttcagc atcttctgtc
    18721 cttacttcag cggcgaacgg ttacgatcaa taccgtagcg ttcgatctct ttatacggca
    18781 cggtttttga ctggcgcttg cgcacatcta ccagcgtcac acggtcggta caggagtagc
    18841 agggatcaag gctaccgata atcagcggtg cgtcagaaac ggtattgccg cgcagcatgt
    18901 aacgcaacac cggccagttg gcgtaggtgg cggcacggca gcgccagcgg aacaattttt
    18961 ggttatcacc gagcatgctc cagtgcacgt cttcaccgcg tggcgcttca acaaagccca
    19021 gcgcgaatgc gtgaggttta tagctaaagc cttcggtcag cagtggggta tccggcatgt
    19081 tgtcgagggc aaattccagc attgccagcg aatcaaacgt ctctttgaca cggaccatca
    19141 cgcgggagaa aacatcgccg ccggtaaagg taaacagtgt ttttggaata ttgccgtagt
    19201 cggcgtaggg gtgatcaaag cgcaaatcac gggcgatttg tcggtcgaga atgccaatgc
    19261 cctgagtgcg ttgttccata ttcggcgtag caagcagcat ctctaccagc tccgacacgt
    19321 cggcgcgcat ctcgcgcacc agtttcagcg tttgcagacg ttgctctttg agaatatcgc
    19381 ggcgaacacc accaatcaga ttcagaccgt aggttttacg cgacccgatc agcaattccg
    19441 ccatcgtcat cgacttttcc cgcacgcgga aaaattgcat aaagccggta tcaaaaccaa
    19501 cgaaatggca ggagaggcca aggttaagca aatgactgtg tagccgttcg acttccagca
    19561 gaatcgagcg aatagtatgt gctcgttgcg gcacctcaat ccccagtgca ttttcaaccg
    19621 agttggtata ggccacactg tgggcaaaac cgcaaatccc acacacgcgg tccgataaga
    19681 aggtcacttc gttgtagccc atccgcgttt ctgccagttt ctccatgccg cgatggacat
    19741 aaaacaggcg gtaatcagca tcgacaattt gctcgccatc cacaaacaag cggaagtgac
    19801 ccggttcatc ggaggtgata tgcagcgggc cgacagggat cacccgcgca tcgctgttgc
    19861 cctcattgat aaacgcatac gtttcggaat ctgtcgtcgg ttcagggcgc agtcgataat
    19921 ccatcgcatc tttgcgcagc ggatgcatat cttccggcca gtcatcgggc aacaccaggc
    19981 gacgctgatc cggcaggcca accggaatca gcccgtacat atcgcgaatt tctcgctcgc
    20041 cccagaccgc ggcagggacg cgcggtgtga ctgacggaaa ctcccgactg tcggcatcga
    20101 ccagtgcctt caccacgatc cagcattttt cggccccttc cattgaaagg gcataataaa
    20161 ccgcgtaatg accgttaagt gtccgctcgt cgttgccaaa caggactgga agccagccat
    20221 catgttgata ataaagataa tgtacaacgt caggcagcag attgattttc accgtaatgg
    20281 tgacctgttc aggcgtttgt cgctcttcat ccagcaccgc gccggggaac tgcgttttca
    20341 gggcggcgag aatcgcttcg ccacgatttg acgatgaatt aacgttcact gtgtttctcc
    20401 gaaacagatg cggtgcctga aggcaaaaaa tcatgccagg tgctacgttg tgcaggcagg
    20461 tcgtgcgtcc ctgagaggac tatagtggaa gcgcccgcca ggatcctgat gacaggttgt
    20521 ggaatatgcg ttcccatcgc cagcatcatg accagcagaa tcaccattgg cgaggtggtc
    20581 aaccagccga gatcaccccg gttaacggcc tgcggcggtt tcgccattaa cacccgcgct
    20641 gccatccgta ccaggcccgc cagcaccagc gttaacagca ataacagcag gacgataatc
    20701 agcaggtgat tacgtgccag tccggcggta atggtcataa attcgctaag aaaaatgttg
    20761 aagggcggca tccctgccag cgccagcgca ccgccgccaa acagcacggc ggtaaatggc
    20821 atgattttga gcatcccaca gacgacgttg agatcgcgcg tgccgtactt gagcagtaca
    20881 ttgccggaac cgcagaacag cagcgttttt gccagactgt ggtttaaggt gtgcagcagc
    20941 gcggcaaaaa ttcccagtgg cccgccaatg cctagcgcca ccgcgaccag tcccatgttc
    21001 tccacgctgg agtacgccag cagacgctta atgtcccgct gtaccagaat gaaaaatgcc
    21061 gccacggcaa ccgacaacat gccgaagatg agcaacaacc ggttggggaa atcgctgccg
    21121 atggcctggc agataatgat gtaatagcga attagcacca acagcgcgca gttcagcaat
    21181 acggcggaga gcagggcgct gaccggactc ggtgcttcac tgtgagcatc cggcagccag
    21241 gcgtgcatgg gaaatagccc ggttttggtg ccaaagccaa ttagcacaaa cacaaaggcc
    21301 aacagcatta atgttgggtc aagcaaggac gattgcttaa gaacctcgct ccagaatatc
    21361 gccatttctg cctgcggcat aacgctggcg acgttggcgt ataccagcac ggtaccgaac
    21421 agaccaaaag cgacaccaac agtacaaata atgatgtact tccatgctgc ttccagcgat
    21481 gaacgctgac cgtaaatgcc taccagaaac gccgagctta aggtggtggc ttcgatcgcc
    21541 gcccacatca caatcaggtt attgctggta acaaccagca gcatggtaaa caaaaacaga
    21601 tggaagaaac cgtagtaatc gcacagcgta acgggggaaa gctcgccgtg tgccacttca
    21661 tgacgcatgt agccaatcga gtaaacaccg gtgagaaagc caatcacacc aagaatggcg
    21721 aggaacaaac cgcccagacc atcaatatga agccacagtc ccgcagcgaa tatttctcct
    21781 gcatcagcgg cagtttggac cacccagagt gccagaatca gcagcagtgt gatccctaag
    21841 ctatgtaata cagtcaccgt acaagtcgca gagagccccc gtttccggca ggcaaaacag
    21901 agcagcgaaa aaagcagcgg cgtgagcagg agtaaagcga acatcacaga ataactcatc
    21961 tcattatccc ttcagcgcgg tcaagttgtt cacgtccagc gtgccgtggg tacgccatat
    22021 ttttcttgcc agtaacacca tcacaatgac ggcgaagatg gcgtcggtag cgatacctat
    22081 ttccaccagt tccggtgctc gccaggcaag aagcgccagc accagatggg agccgttttc
    22141 catcaggcag taaccaaaaa tttgccgcag gatattgcgc tggctgacaa tgcacagcag
    22201 gccaagcaga aaatgaccta acgctaccgc cagcgccggt ttcagcccgg tagccatcgg
    22261 tagcttcacg ggctgaacga caaatgcgca aagcaggaca attaacgccg cgagcagtgc
    22321 catcattgcc ggaccgaata acgctttttc cgggatgttc tggggaatat ttcgtgcagc
    22381 gtaagtcatg attaacggta ccagcagcac tttggtgata aaggcgctgg cggaccagat
    22441 cagcagttgc tctgcggcga acaggcacga gagagtggcg aaaatagaca ccagcaccag
    22501 cgactggcag gcgtaaaatc cgcatgacag gcgatagctt ttgacgctaa tcacaaacag
    22561 cgatgtcagc atcatcagtc ccgccagatt atttacgatc atagaaccgg tcattcatca
    22621 tctccttact gcaaccaggt cgcggcgatt acgctggaaa tcagcgacat cacaatcagt
    22681 acaatcaaca ccaggcgcat tgaccctggc agcggtgcgg catcggcgac ggcctcgctc
    22741 ggtttgccag gcacaacgcg accaaaccag cgaataaacc aggcgaaact ggcgaccgat
    22801 tcaatcatca gcagaatcat ggcgggcagc aggatccagt actccactga caacgcaaaa
    22861 ccggcagcaa acagcgggaa tttactgaag aagccgttga acggcggcac gccggtaatc
    22921 gccagcgctg ccacgcagaa acccacgcct ggcaacggca gggtgtgcaa tacgccacgc
    22981 agacgcggca acaagcgcgt gccgcagctg taactcagcg cacctgctac aaggaaaaac
    23041 aggcttttag cgaacgcgtg gttgacgatg taggcgatgc tgccctccag cgccagccgc
    23101 gagccgaaga tggagagcga caaaccgaag aacatccagc caagttgagt gatggtcgac
    23161 caggccagca accgcttcat atcctgctgt ggcaaataca tcagaaagcc ataaagaatg
    23221 gtgaccagcg ccatgaccat gccaacgccg ccaatcacat gcgggatatt gccgccgtcg
    23281 ataatggcgc gggcaaaaat gtaaacgccc actttcacct tcgatgcggc gtggagatag
    23341 gcactgatcg gtgttggcgc ttccattgcg tccggtagcc acgcttgcat cggtagctgg
    23401 gccgatttcc cccacgcggc aaacaggatg ccgtcataaa ccagataacg tgcgtcgcca
    23461 tgtaactcgc tcatcgcgct aagcacaaac gttccggtct gcaaaaacag cgtggcggcg
    23521 gcaagataca accccaacga accgatatgg gtgataagca acgcttttag tgctgaacgc
    23581 tgcgctttat cgctctggta ataactgatc aacgcccagg agcagccgcc ctaatttcaa
    23641 aaaacaacaa ctgaccgagc agcgtcgagg agagtaccag tcccgccatc gcgccgataa
    23701 acaccagcaa aaatgcgtaa taacgattcg tgccgttatg cgggtgttcg cgatttttat
    23761 ccgtcagata acccgtggag tagatcgtga ccagcaaacc gagaaacacc accacaaaca
    23821 gaatcagcgt actcacgcga tcaatgacca ggccaaacaa tgccacatcc cccacgttga
    23881 ccaacgtcaa cgtgacggca actttatcgg cctgataaaa cgcggagatc agcgacaaca
    23941 tgcacagcgt ggtcagtgcg gcgaacaaaa cccccattcg gcggcccgac gttgtggcga
    24001 aaacgaaacg accagtgcgc cgataaaagg cagcaataac gtcgtcagag caagattttc
    24061 catattccgt cagtgctcct tacagaccgg ttaaccagaa gacccatgca agcgcagcaa
    24121 ggcagaagcc aagccaggtc acatggtgaa tgagtaaaaa acgcccgcgt gccagcgtgt
    24181 tttctgcgat tgaggccagt acaaaaatca gcaaaacctt gagcagcgta acgacaagtg
    24241 aagtcagcag gcaggcgaga gaaagttctt gcgcgcgccc aaaggacaga aacagggcca
    24301 caaacagtga tgccatcacg acctgtttca gccccagccc ccatttcgct agcgccagcc
    24361 cggcaccgga atattcggtc agcgggcctt cctgtaattc ctgttctgct tcagcaacat
    24421 caaagggaat ttttcccatc tcaatgaagc aggcaaaacc acaggccagt aacgccagta
    24481 cggtggttag cggcgagttc cagcccatcg ccagcgtatt gctgatcatc tcgatatgcg
    24541 tggaacctgc tatcagcgcc aataccagca gtgagagaat aagcattggt tcgaccagaa
    24601 tgccgagcgt caactcgcga ctggcaccga ctcccgcaaa cggacttccg gtatccagcc
    24661 cggaaagagc aaagaaaaaa cgaaacaggg caagaagata gataagggtg atcagatcgc
    24721 cgccgcccgc aaaaggggaa acggtaataa acagtggtaa ggccatcgcc agcaccagca
    24781 tgctgctgat taatacccac ggcatcaggc ggaacatcag acctgaagat gtcggcgcaa
    24841 cttcctggcg tttaaacagt ttgtggatat cgcgataatc ctgccagatc cccggcccgc
    24901 ggcgggagtg catacgcgcg cgtatctgcc gggaaataca cgtaaaaagt ggggttagca
    24961 tcagcagaat cacggcctgt gctaacgcaa aaatgaccag atatccgtcg caaagagttt
    25021 gtctcatggt gatttcctta gacggcaata gcgattagca gcacaaccag tgcggcgacc
    25081 acatacaggc aatagagacg aaagtcgccg ctttgcagat gctgtatttc tttggccagc
    25141 cgttgggtgg cgctcacgat ggggcggatc acccgctcat cccagaaggg ttctgtgctc
    25201 tgagccctgg cggtgacgtg cgcaagacct ttatttagcc tcagcgtagg gtcgagctgt
    25261 tgtcgtagac gaaatagcgc agaaaagacc acacgcagcg gctgcatcac gccattgcct
    25321 gacggggcca tcgcattttc ccagccgtag ccgcatgccc aggcatctcc tgtgcgacga
    25381 aacgctgcgc ggcgcgaacg acaccacagc cagtaaaggc caggcagcaa aggcatcgcc
    25441 agtagtaaca gcaacagtaa tgaaggagtg acccgtgtat gaaacgtgcc gggtacaaat
    25501 gctattccgc tggcgacagt gacgggaggg gtattggtaa acgcatgggc aatatgcatt
    25561 atcttcggtg ccagccaact ggcactaagc gcaattaata cgcagagtgc cgcgagcagt
    25621 agcatggcga cgatcatcgt atttggcact tcctgtgcct cttcagcgtg tgtactgcgc
    25681 ggcgcaccac agaaagtaat accgtacatt tttacgaagc acattactgc cagcccacca
    25741 gtgactgcca gcattaccat agcaatagga cccgcaagtt gtagcgctac ggcttccaca
    25801 cgacttagtg agaacagcga ctgccaggtg taccattcgc tgataaaacc attcagcgga
    25861 ggaatggctg atatcgcgag gcaaccaatc aggcatgctg cggctgtcca cggcatccgt
    25921 ttcgccagtg cccccatttt ttccatgtcg tgggtatgca aacgcgaaat aatcgcgccc
    25981 gcgccgagaa atagcagccc tttgaacagc gcatggttga gcagatgaaa cagtgcgccg
    26041 agcagtccaa ccacggtgag cagcgggtcg tgcagtgaca gaccgaccat cgccacaccg
    26101 actgccagca aaataatgcc gacgttttcg acggtactcc aggccagcag ccgtttgata
    26161 tcctgttccg ccagcgcata tagcacgccc aggagcgcgg agattgcgcc gatcgccatc
    26221 accagaatgc cccaccacag aggcaaaccc gtttgcgcca gcagatccat cgctactttc
    26281 aggatgccga aaataccgat tttgaccatt acgccagaca tcaacgccga agcgtgcgat
    26341 ggtgcggcag ggtgagcgcg cggcaaccag ctgtgcaacg gcatcatccc ggctttcgcg
    26401 ccaaaaccga aaaaggccag cagaaacacc gccgacgcca gccccggaga aagtgaaagc
    26461 gtgcggaaac tggcaaaatc gaggctgccg ctttcgcgcc ccatcagcaa gaaggcgatc
    26521 ataatcagca ccgatccggc gtgggcgata aaaaagtaga gcatgccagc gttgatcgac
    26581 gttttatcct gcctggcaat gaccagaaac caggaagaca gcgacatcat ttcaaacagc
    26641 acgatgaacc aaaaagtgtt gtccatcacc agcagggcaa ccatcgatgc gatgaaaaga
    26701 ttcataaaga agcccatcgc cgccgcgcct ttgccctcgt attcgcgcat ataagtcaat
    26761 gaatagagcg aacaaaccac caccagcaag gagataacca gcaccataaa tgccgagagg
    26821 ctatccattc gcagggttaa atcggcaaat tcaaaagggg tggcaaaacg ggcgactaaa
    26881 gttacgcctg catgtaattg cgtaattccg ctgatgatgc caatcacgcc gcccaccagc
    26941 gatgtgatgc cggaaagctt aatagccagt ctgtccagac cgagtaaaaa cagcgaagcc
    27001 agactggcaa acagatagag gatcagcgac caggttaata attgcagggc atccattagc
    27061 gttgctcctg agtgagggaa aggggatcct ccccgccatt ggcaaaccag cttgcggcaa
    27121 ggcgctgttt ttctttcatc tgacgttgca ggctatcgtc ggtgatcagc cgtaacgcct
    27181 gattcgggca agcgcgaacg caggccggac cttctggcaa gaaatcacac aggtcgcatt
    27241 tcaccgcgac ggtctggaca cccggttccc agcgcagcaa aggatgttgg ggcggcgcgt
    27301 tttcttcgcc gtctttgagt gagccctcag cctgaaaaac atattgcgca ggggcattca
    27361 ccggacggct tcctgaagcg ctgattgcgc caaatgggca gaccactgcg caaagcttgc
    27421 agccaataca gaggctttcg ttgagttgga tcgcatcatc cctctgggag atggcattga
    27481 ccgggcagac ctgcaggcaa ggggcttcct cacagtgatg acacacgaca ggggcagtga
    27541 gtgttgacgt cttcgccagg gccaggcgcg ggtgttgctg taaaccttgc gttttatgca
    27601 cgtccgaaca ggcagcgaga caggtattac atcctgtaca ccacagtggt tcggccacca
    27661 caaagcggtt catgtcggct ccttgaaaat gatatgaaac attgcatttc atattgcata
    27721 aaggatgcca gtctgattaa ctcaatttat tcaataagtt atgtattaac tggattctga
    27781 acggtacaaa aatgatggtc attaacgttc agcaataaat ggattcgaca cccgtgtcgc
    27841 aggggaaagt gagaatgtga tcttgatcac gagaagtgag gtgtgctgac cgcaagcgca
    27901 gccagcacgg aatggagcaa agtaatcagg cgtgttcttt cagccagttc agcacaacgt
    27961 cgtggtgatt gctggttttg aaatcgtcaa agacatgttc gattttgcca tcagcgtcaa
    28021 tcaggaagct gatgcgatga atgccatcgt aggttttgcc catgaaggac ttttcacccc
    28081 agacgccgaa ttgttcgcac acctggtggt cctcatcaga caggagcgta aagttaagca
    28141 gctctttttc cgcaaaacgg gagagttttt cgggtttatc ggtgctgata cccagcacat
    28201 caacgcccgc ttttttcaac tcatccatgt tatcgcgtaa gccgcaggcc tgtacggtac
    28261 agccgggggt catggctttc gggtagaaat aaaccagaac acgctgtccc tggaagtcgg
    28321 tcaaattaac ttgttctccg tcttgatccg gcaagctaaa tttcggtgcg atatcaccgg
    28381 ctttcagtgg attcattact taactccatc ctgttcatca tgttgggaat aattgacgac
    28441 gttaatactg ccttgtgcat tgagttctgt acatagggct ttgaacgctt gctcaatatt
    28501 tgctgcgtcc gcagatgcgg ggctgtgggc ggttatctga atatgcaact gcgcagcccg
    28561 ttcattttca gcaggttgcg tgcgcgacac cagctccgca atgttcatat gatgcgtgtc
    28621 gaaaagtgct gtgaagcgtt caattaaatg cggggagtct gccacatcga cctgaaccca
    28681 gacagatgct ggcattggcg gacgcggacg cgccgtcgtg cgcttcatca cgattaaaag
    28741 atccagctcg gcacctttca acggtaacgt tgattcaatc agagtaatgg cattccatga
    28801 accggaaagc agcatgataa acgtgaactc ttctcccagc atcgccaggc gactgtcttc
    28861 aatattacag ccgcaactac tgacatgacg ggtgatggtg ttcacaattc cagggcgatc
    28921 ggcacccaac gcagtgatca ccagataatg ttgcgatgac agtgtcaaac tggttattcc
    28981 tttaaggggt gagttgttct taaggaaagc ataaaaaaaa catgcataca acaatcagaa
    29041 cggttctgtc tgcttgcttt taatgccata ccaaacgtac cattgagaca cttgtttgca
    29101 cagaggatgg cccatgttca cgggaagtat tgtcgcgatt gttactccga tggatgaaaa
    29161 aggtaatgtc tgtcgggcta gcttgaaaaa actgattgat tatcatgtcg ccagcggtac
    29221 ttcggcgatc gtttctgttg gcaccactgg cgagtccgct accttaaatc atgacgaaca
    29281 tgctgatgtg gtgatgatga cgctggagct ggctgatggg cgcattccgg taattgccgg
    29341 gaccggcgct aacgctactg cggaagccat tagcctgacg cagcgcttca atgacagtgg
    29401 tatcgtcggc tgcctgacgg taacccctta ctacaatcgt ccgtcgcaag aaggtttgta
    29461 tcagcatttc aaagccatcg ctgagcatac tgacctgccg caaattctgt ataatgtgcc
    29521 gtcccgtact ggctgcgatc tgctcccgga aacggtgggc cgtctggcga aagtaaaaaa
    29581 tattatcgga atcaaagagg caacagggaa cttaacgcgt gtaaaccaga tcaaagagct
    29641 ggtttcagat gattttgttc tgctgagcgg cgatgatgcg agcgcgctgg acttcatgca
    29701 attgggcggt catggggtta tttccgttac ggctaacgtc gcagcgcgtg atatggccca
    29761 gatgtgcaaa ctggcagcag aagggcattt tgccgaggca cgcgttatta atcagcgtct
    29821 gatgccatta cacaacaaac tatttgtcga acccaatcca atcccggtga aatgggcatg
    29881 taaggaactg ggtcttgtgg cgaccgatac gctgcgcctg ccaatgacac caatcaccga
    29941 cagtggtcgt gagacggtca gagcggcgct taagcatgcc ggtttgctgt aaagtttagg
    30001 gagatttgat ggcttactct gttcaaaagt cgcgcctggc aaaggttgcg ggtgtttcgc
    30061 ttgttttatt actcgctgcc tgtagttctg actcacgcta taagcgtcag gtcagtggtg
    30121 atgaagccta cctggaagcg gcaccgcttg cggagcttca tgccccggct ggaatgattt
    30181 tgccggtgac ctccggtgat tatgcaatcc cggtgaccaa cggtagtggt gctgtcggta
    30241 aggcgctgga cattcgtcca ccagcccagc cgttggcact ggtttctggc gcgcgtaccc
    30301 agttcacggg cgataccgct tcattgctgg tggaaaatgg tcgtggcaat actctgtggc
    30361 cgcaggtggt tagcgtgctg caggcgaaaa actacaccat cacccaacgt gatgatgctg
    30421 gtcagacact gaccaccgat tgggtacaat ggaaccgtct ggacgaagac gagcagtatc
    30481 gtggtcgtta tcaaatctct gttaagccgc agggttatca gcaggcggtt acggttaaac
    30541 tgctgaacct ggaacaggcg ggcaaaccgg ttgcagacgc ggcttccatg cagcgttaca
    30601 gcacggagat gatgaacgtt atttccgccg gtctggataa atctgccact gacgccgcga
    30661 acgctgcgca aaatcgtgcc tccaccacta tggacgtaca aagtgcagct gatgacaccg
    30721 gtttaccaat gctggtcgta cgcgggccgt tcaatgtggt ttggcaacgt ctgccagcgg
    30781 cgctggaaaa agtgggcatg aaagtgaccg acagcacccg ttcgcagggc aacatggccg
    30841 taacttataa gccgctgtct gacagcgact ggcaggaact gggcgcgagc gatccaggcc
    30901 tggcatccgg tgactataaa ctgcaggttg gcgatttaga taaccgcagc agcctacagt
    30961 tcatcgatcc gaaaggtcat actctgactc agagtcagaa cgacgcgctg gtagctgtct
    31021 tccaggctgc gtttagcaag taaaaataca gggctggaat catccggccc ttttttctga
    31081 tatgatacgc aaacgtgtgc gtcggcagga aaacgcgatt ttagcggtaa ttcgcacgaa
    31141 atttgtttgt cggacgtagt tcggataagg cgttcacgcc gcatccgaca aaacatccgg
    31201 cacaccagac agcaaaagat tttaaaacgt taattcacac ccaggagtga taaagatgca
    31261 aaagcaagct gagttgtatc gtggtaaagc gaaaaccgta tacagcacgg aaaacccgga
    31321 cctgttggtg ctcgaattcc gcaatgatac gtcagcaggg gatggcgcgc gcattgagca
    31381 gtttgatcgc aaaggtatgg tgaacaacaa gttcaactac ttcatcatga gcaaactggc
    31441 tgaagcgggt atcccgactc aaatggagcg tctgctctcc gataccgaat gtctggtgaa
    31501 aaagctggat atggtgccgg ttgagtgtgt cgtgcgtaac cgtgctgctg gctctctggt
    31561 gaaacgtctt ggaatcgaag aaggtattga gctgaacccg ccgctgttcg atctgttcct
    31621 gaaaaacgac gccatgcacg atccgatggt caacgaatct tactgcgaaa cctttggctg
    31681 ggtgagcaaa gagaacctgg cgcgtatgaa agagctgacc tacaaagcga acgacgtgct
    31741 gaaaaaactg ttcgatgatg ctggtctgat tctggtcgac ttcaagctgg agtttggcct
    31801 gtacaaaggt gaagtggtac tgggcgatga gttctccccg gacggcagcc gcctgtggga
    31861 caaagaaaca ctggagaaaa tggacaaaga ccgtttccgc cagagcctcg gtggcctgat
    31921 cgaagcctat gaagccgtcg cccgccgcct gggtgtacag ctggactgat ttttctggtt
    31981 gcttgtttct ttagccggag ggtactccgg ctaaatgtct ttgatacccc cgctacaata
    32041 cttctcatta tctccctttg acaggaaagt cataccaggc caatatgtat atacataacg
    32101 tatatcccaa aaaggggggg ctatggaaac tactgtattt ttaagtaatc gcagtcaggc
    32161 cgtcagatta ccgaaagcgg tcgctctgcc agaagacgtt aagaaagtag aaattattgc
    32221 gattggtcgt acaagaatca tcactcccgc cggtgagtcc tgggacagtt ggtttgatgg
    32281 cgaaaatgtg agtgcggatt ttatggatat ccgtgatcaa cccgcaatgc aggaaaggga
    32341 atcattttga tgctgaaatt catgctggat accaatatct gtattttcac tattaagaat
    32401 aaaccagtac atgtcagaga gcggtttaac ctgaattctg gccggatgtg tataagttct
    32461 gtaacattga tggagcttat ctatggggca gaaaaaagtc agatgccaga acgcaatttg
    32521 gcggttattg aaggtttcgt atctcgtttg gtagtgcttg attatgatag tgctgctgcg
    32581 acgcataccg ggcaaatcag ggccgagctt gcccggcagg gaaggcctgt tggacctttt
    32641 gaccagatga tagcgggaca tgctcgcagt cgcggtttga ttgtggttac caacaatacc
    32701 cgagagtttg agcgagtggc gggaatcaga ctagaagact ggagctaaca ttctctttta
    32761 cttctaacat tcgtaaatta tataacgtgg catgaagcct taattcctcc gccatttcat
    32821 cccgctttcc tgtggtaatc ttcctctgaa ccacctacga tcatttctat aatgaatata
    32881 ttgttgaggt atctatgcgt tggcaagggc gacgtgaaag tgacaatgtt gaagacaggc
    32941 gcaacagctc tggcggtccg tcaatgggcg gtcccggttt ccgtctgccg agcggtaaag
    33001 gcgggctgat tttactgata gtcgtgctgg ttgcaggcta ctatggcgtt gatttaaccg
    33061 ggttgatgac cgggcagccg gtttctcaac aacaatcaac gcggtcaatt agccctaatg
    33121 aagacgaagc cgcaaaattc acctcggtga ttctggcaac cacggaagac acctggggac
    33181 aacagttcga gaagatgggt aagacctatc agcaaccgaa gctggtcatg taccgtggaa
    33241 tgacgcgcac cggctgcggg gctggtcagt ccattatggg gccgttctat tgcccggcgg
    33301 atggcacggt ttatatcgat ctctccttct atgatgacat gaaagacaaa cttggcgcgg
    33361 atggcgattt tgcccagggg tacgttatcg cccatgaagt cggtcatcat gtgcagaaac
    33421 tgttaggcat cgagccgaaa gttcgtcaac tgcaacaaaa cgcgacgcag gcggaagtaa
    33481 accgcttatc tgtgcgtatg gaactccagg ccgactgttt tgccggtgtc tgggggcata
    33541 gtatgcagca gcaaggcgtt ctggaaactg gcgatctgga agaggcgctg aatgcggcgc
    33601 aggccatcgg cgatgaccgt ttacaacagc aaagtcaggg gcgagtagta ccagacagtt
    33661 tcacacatgg cacttctcag caacgctaca gctggtttaa acgtggtttc gacagcggcg
    33721 atccggcaca atgcaatact tttggtaaaa gcatttaatt tccggggcag ggatggctga
    33781 actgactgcg ctttacacat taacagcgca aatgaaacgt gaagggatcc gccgcttgct
    33841 ggtgttgagc ggggaagagg gttggtgttt tgatcatgct cttaagttgc gtgatgcctt
    33901 acctggcgac tggctgtgga tttcgccgca gctagatgct gaaaaccact gttcaccctc
    33961 ggcgctacaa actttacttg ggcgcgagtt ccggcatgcg gtattcgacg cccgccacgg
    34021 ctttgatgcc gctgcctttg cagcacttag cggaacgttg aaagcgggaa gctggctagt
    34081 tttgttactc cctgtatggg aagagtggga aaaccaacct gatgccgact cgctgcgctg
    34141 gagtgattgc cctgacccta ttgcgacgcc gcattttgtc cagcatctca aacgcgtact
    34201 tacggcggat aacgacgcta tcctctggcg gcaaaaccag ccgttctcgt tggcgcattt
    34261 tactccccgt actgactggc accccgcgac cggcgcacca caaccagaac aacagcaact
    34321 cttacagcag ttactgacca tgccgccggg cgtggcagcg gtaacggctg cgcgtgggcg
    34381 cggtaaatcg gcgttggcag ggcaactcat ttctcgtatt gcgggcagtg cgattgtcac
    34441 cgcgcccgca aaagcggcaa cggatgtact ggcacaattt gcgggcgaga agtttcgctt
    34501 tattgcgccg gatgccttgt tagccagcga tgagcaagcc gactggctgg tggtcgacga
    34561 agccgcagcc atacctgcgc cgttgttgca tcaactggta tcgcgttttc ctcgaacgtt
    34621 gttaaccact acggtgcagg gctacgaagg caccggacgt ggttttttgc tgaaattttg
    34681 cgctcgcttt ccgcatttac accgttttga actgcaacaa ccgatccgct gggcacaggg
    34741 atgcccgctt gaaaaaatgg ttagtgaggc actggttttt gacgatgaaa acttcaccca
    34801 tacaccacaa ggcaatatcg tcatttccgc atttgaacaa acgttatggc gaagcgagcc
    34861 agaaacgccg ttaaaggttt atcagttatt gtctggtgcg cactaccgga cctcgccgct
    34921 ggatttacgc cggatgatgg atgcaccagg gcaacatttt ttacaggcgg ctggcgaaaa
    34981 cgagattgcc ggagcgctgt ggctggtgga tgagggggga ttatctcaag aactcagtca
    35041 ggcggtatgg gcaggttttc gtcgcccgcg gggtaatctg gtggcccagt cgctggcggc
    35101 gcacggcagc aatccactgg cggcgacatt gcgcggaagg cgggttagcc ggatagcagt
    35161 tcatccggct cgtcagcggg aaggcacagg gcggcaactt attgctggtg ctttgcaata
    35221 tacgcatgac ctcgactatc tttcggtgag ttttggttac accggggagt tatggcgttt
    35281 ctggcaacgc tgcggttttg tgctggtgcg aatgggtaat catcgtgaag ccagcagcgg
    35341 ttgctatacg gcgatggcgc tgttaccgat gagtgatgcg ggtaaacagc tggctgaacg
    35401 tgagcattac cgtttacgtc gcgatgcgca agctctcgcg cagtggaatg gcgaaatgct
    35461 tcctgttgat ccactaaacg atgccgtcct ttctgacgac gactggcttg aactggccgg
    35521 ttttgctttc gctcatcgtc cgctattaac atcgttaggt tgcttattgc gtctgctaca
    35581 gaccagcgaa ctggcattac cggcgctgcg tgggcgttta cagaaaaacg tcagcgacgc
    35641 gcagttatgt accacactta aactttcagg ccgcaagatg ttactggtcc gtcagcggga
    35701 agaggccgcg caggcgctgt tcgcacttaa tgaggttcgc actgagcgtc tgcgcgatcg
    35761 cataacgcaa tggcaatttt ttcactgact ccttcaattt tctcccatgg tcattgtgag
    35821 tcagcggcgt aaaattaatg acatcagtta aggagttcgc catgaaacat gaccattttg
    35881 ttgttcaaag cccggataaa cctgcgcaac agttgttact gcttttccac ggtgtcgggg
    35941 ataacccggt ggcaatgggg gaaataggca gctggtttgc gccgctattt cctgatgcgc
    36001 tggtggtgag tgtcggaggc gcggagccga gcggtaatcc ggcggggcgt cagtggtttt
    36061 cggtgcaggg tattacggaa gataatcgcc aggcgcgtgt ggatgccatc atgccgacgt
    36121 ttattgagac ggtacgctac tggcagaaac agagcggagt aggggctaac gccacggcgc
    36181 taattggttt ctcacaaggc gcgattatgg cgctggagag cattaaagcc gaaccgggcc
    36241 ttgcttcgcg cgtcattgcc tttaacggac gctatgcgag cctgccggaa accgcgtcta
    36301 cagccacgac aattcacctg attcatggtg gtgaagatcc ggttatcgat ctggcgcatg
    36361 cagttgcggc gcaggaagcg ttaatcagtg ccggtggtga cgtgacgctg gatatcgtgg
    36421 aggatttagg ccatgcgatc gacaatcgca gtatgcaatt tgccctggat cacttgcgtt
    36481 ataccattcc gaaacattat ttcgacgaag cgctaagtgg cggtaagcct ggcgatgatg
    36541 atgtgattga aatgatgtaa taatagccgg gtaggcctga taagactgta tcaggcatca
    36601 tggccggatg tggcgtgaac gccttatccg gccactcagc ttacttcttc ggttgatcct
    36661 ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc gggggaagct
    36721 ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta atcacgttca
    36781 gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc agccagtcca
    36841 tgcttatttc ctcttgcaga actactcatc aggcgacgag ctgttccatg atacgttgat
    36901 acatacgggc aagtagctgc aggtcggcag cgttcacaca ttcattaatt ttatgaatag
    36961 tggcattgac cggcccgagt tccaccacct gcgcccccat gcgagcaata aagcgcccgt
    37021 cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc tcaaccgcgt
    37081 taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg gaaagccacc
    37141 aatccaccgt atagcgcagt tgatgttttt caagcagggc aagtacctgc gctttgatca
    37201 tctcatccgt cagttcggtg ctgaagcgga agttaaactg cacaaacagt tcgcccggaa
    37261 taacgttgtt actgcccgtt cccgcctgaa tattggcaat ctgcatactg gtcgccggga
    37321 agaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa ggtgctgcgc
    37381 gatgtaccgg attgtcagcc agatgtgggt aggcaacatg cccctgaacg ccatgaatgg
    37441 tgaggttgca ggttaatgat ccgcgacgac catttttcac cacatcacct accacttcga
    37501 tactcgacgg ttcgccgacc aggcagtaat cgagacgctc attacgtgcc attaacgctt
    37561 cgacgacttt taccgtaccg ttgtgggcac tggcttcttc atcagaggtg atcagaaatg
    37621 ccagtcgccc cgtatggttg ggatgttgtg cgacaaaacg ctctgctgct accaccatcg
    37681 ccgccagcga gcctttcata tctgccgcgc cgcgcccgaa taacatgccg tcacgaatgg
    37741 tcggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc acgtcggtat
    37801 gcccggcaaa ggctaacgtt tcaccttgcc cacgccatgc ccaaaaattc tgcgtatcgg
    37861 caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc aacaaagcct
    37921 ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt gtcagctcaa
    37981 taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg gaatcactga
    38041 aacccagcag cataggctta ccaggcgcgc agagcaatgg acgtttgata attgcaggca
    38101 tttcagtcat taatgccgcc gcagaggccg catcggtgat tttattgcgg gtggtttcgt
    38161 ccagtttacg ccaggttgta ccacgggtgt tcagtaacgc ttcccagcct aattcgttga
    38221 taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga aaacgatagt
    38281 cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa tttttgatgc
    38341 cgtaaagtgt aaccataatg aatccttttt cgatctatga gaataaatac tcatttaagg
    38401 gtgaaaacat tttgcctgca aat
//
LOCUS       DAZGXZ010000031        37708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000031 DAZGXZ010000000
VERSION     DAZGXZ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.031"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            160..297
                     /locus_tag="ACHKJF_001730"
                     /pseudo
     CDS             160..297
                     /locus_tag="ACHKJF_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(405..1640)
                     /gene="aslB"
                     /locus_tag="ACHKJF_001731"
     CDS             complement(405..1640)
                     /gene="aslB"
                     /locus_tag="ACHKJF_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0949108.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1787..1863)
                     /locus_tag="ACHKJF_001732"
     tRNA            complement(1787..1863)
                     /locus_tag="ACHKJF_001732"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1827..1829),aa:Pro,seq:tgg)
     gene            complement(1906..1992)
                     /locus_tag="ACHKJF_001733"
     tRNA            complement(1906..1992)
                     /locus_tag="ACHKJF_001733"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1956..1958),aa:Leu,seq:cag)
     gene            complement(2013..2088)
                     /locus_tag="ACHKJF_001734"
     tRNA            complement(2013..2088)
                     /locus_tag="ACHKJF_001734"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2053..2055),aa:His,seq:gtg)
     gene            complement(2147..2223)
                     /locus_tag="ACHKJF_001735"
     tRNA            complement(2147..2223)
                     /locus_tag="ACHKJF_001735"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2187..2189),aa:Arg,seq:ccg)
     gene            complement(2326..3711)
                     /gene="yifK"
                     /locus_tag="ACHKJF_001736"
     CDS             complement(2326..3711)
                     /gene="yifK"
                     /locus_tag="ACHKJF_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0949109.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLVYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRYGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3902..4642)
                     /gene="wecG"
                     /locus_tag="ACHKJF_001737"
     CDS             complement(3902..4642)
                     /gene="wecG"
                     /locus_tag="ACHKJF_001737"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP0949110.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4645..5997)
                     /gene="wzyE"
                     /locus_tag="ACHKJF_001738"
     CDS             complement(4645..5997)
                     /gene="wzyE"
                     /locus_tag="ACHKJF_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP0949111.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5994..7073)
                     /gene="rffT"
                     /locus_tag="ACHKJF_001739"
                     /gene_synonym="wecF"
     CDS             complement(5994..7073)
                     /gene="rffT"
                     /locus_tag="ACHKJF_001739"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP0949112.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7070..8320)
                     /gene="wzxE"
                     /locus_tag="ACHKJF_001740"
     CDS             complement(7070..8320)
                     /gene="wzxE"
                     /locus_tag="ACHKJF_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP0949113.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8322..9452)
                     /gene="rffA"
                     /locus_tag="ACHKJF_001741"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8322..9452)
                     /gene="rffA"
                     /locus_tag="ACHKJF_001741"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP0949114.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVIST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9457..10131)
                     /gene="rffC"
                     /locus_tag="ACHKJF_001742"
                     /gene_synonym="wecD"
     CDS             complement(9457..10131)
                     /gene="rffC"
                     /locus_tag="ACHKJF_001742"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP0949115.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10109..10990)
                     /gene="rfbA"
                     /locus_tag="ACHKJF_001743"
     CDS             complement(10109..10990)
                     /gene="rfbA"
                     /locus_tag="ACHKJF_001743"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0949116.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11009..12076)
                     /gene="rffG"
                     /locus_tag="ACHKJF_001744"
     CDS             complement(11009..12076)
                     /gene="rffG"
                     /locus_tag="ACHKJF_001744"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0949117.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12076..13338)
                     /gene="wecC"
                     /locus_tag="ACHKJF_001745"
     CDS             complement(12076..13338)
                     /gene="wecC"
                     /locus_tag="ACHKJF_001745"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP0949118.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13335..14465)
                     /gene="wecB"
                     /locus_tag="ACHKJF_001746"
     CDS             complement(13335..14465)
                     /gene="wecB"
                     /locus_tag="ACHKJF_001746"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP0949119.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14521..15567)
                     /gene="wzzE"
                     /locus_tag="ACHKJF_001747"
     CDS             complement(14521..15567)
                     /gene="wzzE"
                     /locus_tag="ACHKJF_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP0949120.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15579..16682)
                     /gene="wecA"
                     /locus_tag="ACHKJF_001748"
     CDS             complement(15579..16682)
                     /gene="wecA"
                     /locus_tag="ACHKJF_001748"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP0949121.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16922..18181)
                     /gene="rho"
                     /locus_tag="ACHKJF_001749"
     CDS             complement(16922..18181)
                     /gene="rho"
                     /locus_tag="ACHKJF_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP0949122.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18266..18367)
                     /gene="rhoL"
                     /locus_tag="ACHKJF_001750"
     CDS             complement(18266..18367)
                     /gene="rhoL"
                     /locus_tag="ACHKJF_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP0949123.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18508..18837)
                     /gene="trxA"
                     /locus_tag="ACHKJF_001751"
     CDS             complement(18508..18837)
                     /gene="trxA"
                     /locus_tag="ACHKJF_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP0949124.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18968..20233
                     /gene="rhlB"
                     /locus_tag="ACHKJF_001752"
     CDS             18968..20233
                     /gene="rhlB"
                     /locus_tag="ACHKJF_001752"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP0949125.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKYRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20367..21851
                     /gene="gppA"
                     /locus_tag="ACHKJF_001753"
     CDS             20367..21851
                     /gene="gppA"
                     /locus_tag="ACHKJF_001753"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP0949126.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21898..23919)
                     /gene="rep"
                     /locus_tag="ACHKJF_001754"
     CDS             complement(21898..23919)
                     /gene="rep"
                     /locus_tag="ACHKJF_001754"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP0949127.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGRELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24136..24585
                     /locus_tag="ACHKJF_001755"
     CDS             24136..24585
                     /locus_tag="ACHKJF_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP0949128.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24784..25065
                     /gene="ppiC"
                     /locus_tag="ACHKJF_001756"
     CDS             24784..25065
                     /gene="ppiC"
                     /locus_tag="ACHKJF_001756"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP0949129.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25152..26627)
                     /gene="ilvC"
                     /locus_tag="ACHKJF_001757"
     CDS             complement(25152..26627)
                     /gene="ilvC"
                     /locus_tag="ACHKJF_001757"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP0949130.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26777..27667
                     /gene="ilvY"
                     /locus_tag="ACHKJF_001758"
     CDS             26777..27667
                     /gene="ilvY"
                     /locus_tag="ACHKJF_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP0949131.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(27664..29208)
                     /gene="ilvA"
                     /locus_tag="ACHKJF_001759"
     CDS             complement(27664..29208)
                     /gene="ilvA"
                     /locus_tag="ACHKJF_001759"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP0949132.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29211..31061)
                     /gene="ilvD"
                     /locus_tag="ACHKJF_001760"
     CDS             complement(29211..31061)
                     /gene="ilvD"
                     /locus_tag="ACHKJF_001760"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP0949133.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31126..32055)
                     /gene="ilvE"
                     /locus_tag="ACHKJF_001761"
     CDS             complement(31126..32055)
                     /gene="ilvE"
                     /locus_tag="ACHKJF_001761"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP0949134.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32075..32338)
                     /gene="ilvM"
                     /locus_tag="ACHKJF_001762"
     CDS             complement(32075..32338)
                     /gene="ilvM"
                     /locus_tag="ACHKJF_001762"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP0949135.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32335..33981)
                     /gene="ilvG"
                     /locus_tag="ACHKJF_001763"
     CDS             complement(32335..33981)
                     /gene="ilvG"
                     /locus_tag="ACHKJF_001763"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP0949136.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(33984..34034)
                     /gene="ilvX"
                     /locus_tag="ACHKJF_001764"
     CDS             complement(33984..34034)
                     /gene="ilvX"
                     /locus_tag="ACHKJF_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP0949137.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34121..34219)
                     /gene="ilvL"
                     /locus_tag="ACHKJF_001765"
     CDS             complement(34121..34219)
                     /gene="ilvL"
                     /locus_tag="ACHKJF_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP0949138.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34572..36092
                     /gene="yifB"
                     /locus_tag="ACHKJF_001766"
     CDS             34572..36092
                     /gene="yifB"
                     /locus_tag="ACHKJF_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP0949139.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36117..36455)
                     /gene="maoP"
                     /locus_tag="ACHKJF_001767"
     CDS             complement(36117..36455)
                     /gene="maoP"
                     /locus_tag="ACHKJF_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP0949140.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36574..37413
                     /gene="hdfR"
                     /locus_tag="ACHKJF_001768"
     CDS             36574..37413
                     /gene="hdfR"
                     /locus_tag="ACHKJF_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP0949141.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDELSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(37509..37584)
                     /locus_tag="ACHKJF_001769"
     tRNA            complement(37509..37584)
                     /locus_tag="ACHKJF_001769"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37549..37551),aa:Trp,seq:cca)
     gene            complement(37593..37669)
                     /locus_tag="ACHKJF_001770"
     tRNA            complement(37593..37669)
                     /locus_tag="ACHKJF_001770"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37633..37635),aa:Asp,seq:gtc)
BASE COUNT         9229 a        10270 c         9483 g         8726 t
ORIGIN      
        1 tgaaaacgct ctcctgattt tccaattcat tttctggata aataaataat ttatttttgt
       61 cactattatt tatgtaatca tcctgtcagg gagagggatc tcaattatca atgcttaatt
      121 acgtcatcat tttgatgaca tgaaaccgga gcattttgca tggaattttc gttttcaccc
      181 aaacttcttg ttgttgctgt cgccgccgct cttcctctca tggccagcgc agcagatacc
      241 ccgtcaactg ccaccgcacg caaaggcttt gccggatacg atcacccaaa ccagtatcca
      301 cagaacgctg caatttattg aatttgcacg atcatgtagg ccggataagg cgtttacgcc
      361 gcatccggca atcaaccgca ggcggccaca aaatggccgc ttttttactt attcaccacc
      421 agcaaatgtg cctgcataat gtcactggcc gggcgaccgt gcgccagcaa atcagccatt
      481 gctttaagat atggcggtag atggcggaaa taacgctgat acccggcaca caaataattc
      541 agccccggtt tgccgctggc atcgagcatg aagcggtgtt tcgggcagcc tccccagcac
      601 gcttttaaca cgttacaact gcgacactgc gccggtaatt gcttaaattt atcttcacca
      661 aacacctgct gttgcgggga atcgatcatt tctgcaattg tttgctgatg catattcccc
      721 agccgatatt gcggataaac atagtgatcg caggcgtaaa cgtcgccgtt gtgctcaaca
      781 atcaccgagc gcccacaggt tggctgatga tggcaaaccg cacccggcgc accgacaaaa
      841 ttggcaaacg cccattcgat attcatcacg aaaatcttgc cgacgtcgcg tttgatccag
      901 tggtcgaata tcgccaccag aaattcacca aattcatcgg ggcgcaccga ccattccgtc
      961 agttccccct gaatatcacc aggcgcatgt agtttcagtc catcgctggc agctgtttca
     1021 tcagccaggc gctcgaccac cggaataaac tggatgaatt cgactcccgc atcgcacaaa
     1081 aaatcataaa cctgcaacgg ttgctgcgcg ctggtgcggt tgacgcagac cagcacgtta
     1141 tagtcgacat gatgtttttg cagcagcgtc agggcacgca tcaccagctt atgcgttggt
     1201 ctgccacctt tggtcacgcg atattgattg tggatctcag ccggaccgtc cagcgataac
     1261 ccaacaagaa aatgattttc tgccagaaat gcacaccatt tatcatcgag cagcacgccg
     1321 ttagtctgga agctgttact tatcttcctg ccagcaccgt atttcgcctg taactttacg
     1381 gcacggcggt aaaaatccag cccgagtaac gttggttcgc cgccctgcca ggtaaaagcc
     1441 acttcgtttt gcggttcgct ggcagcgata tagtgacgga catacgcttc cagcgtgtca
     1501 tcgtccatat gcgtgactgg cttttcgcgg taaagggatt gtttttcgag ataaaaacag
     1561 tagtcacagt tcagattaca atcggaacca ctcggtttcg ccatcacatg aaaagcacgc
     1621 gttggaacct gttgcagcat ggtcactcct tgcctgaagt catccttcaa tacgttaagg
     1681 gcgaaatcat accgtggggc aatgagttat tacgggcgtt tattgcgaag ggagggtgac
     1741 gaaatgcaca gaaaacaaaa aaccccgccg aagcggggtt caaaattggt cggcgagaga
     1801 ggattcgaac ctccgaccca ctggtcccaa accagttgcg ctaccaagct gcgctactcg
     1861 ccgaaatact gctttttgaa tttttagttc aattctttaa agtcgtggtg cgaggggggg
     1921 gacttgaacc cccacgtccg taaggacact aacacctgaa gctagcgcgt ctaccaattc
     1981 cgccaccttc gcattgtcac aacttctaat aatggggtgg ctaatgggat tcgaacccac
     2041 gacaactgga atcacaatcc agggctctac caactgagct atagccacca ctacaaatct
     2101 tgttacgcgg tattactacc accgcagctc aagcgccggg actaaatggc gcgcccgaca
     2161 ggattcgaac ctgagacctc tgcctccgga gggcagcgct ctatccagct gagctacggg
     2221 cgcttagcgc cgttgcgggg gtggataata cggacttccc acccctctgt ctagtccctt
     2281 tttaaataaa ttgcgcgttt ggttacggtt tgtgcgtttt gctgcttatt cctccagttt
     2341 atgcgctttc ccgtggcgat taaggccaaa aactttataa atcgccgtca ccgccagcat
     2401 aaagatgatg ccaacaaaca gcgacatacg cgtatcttca ttaaagtaca tgccgatcaa
     2461 aacgcaaatc aggaatgcca ttgttacgta gttggcccac gggaacagga ttgagcggaa
     2521 cggatggctg gcaatcgccg ctttatgtgc acgccgaaaa cgcagctggc ttatcaatat
     2581 cacaaaccat ggcaccatcc ccggaagcac gctggcactg tagacgtaga caaacacacg
     2641 ctgcggattg ggaatgatgt agttcaggca tgagccaatt agcagaatag caatagatac
     2701 tgccacaccc gcaacgggta cgccgtaacg ggaaactttc gccatcgccg ccggtaactg
     2761 acggtttttc gccagtgcgt agagcatacg tccgcaactg tacatgccgc tgttacagcc
     2821 agagagcgca gccgtcagca ccacaaagtt gataatgccc gccgctgcgg taataccgat
     2881 tttggcaaaa gtcagtacga acgggctgcc gttgctgcct atttcattcc acgggaagat
     2941 ggtgacgata acgaaaatcg cgcctacgta gaaaatcagg atccgccaca gcaccttgcc
     3001 tacggcactg cgcagcgtca cctgcggatt cttcgcttca ccggcagtaa tgccaatcag
     3061 ctccacgccc tggtaggacg ccaccacaat acacagcgcg gtcaggaacc ctttccagcc
     3121 acccgcaaag aaaccgccat gctctgtgag attgctaaaa ccaatcgact gcccgccatt
     3181 gccaaagcca aagaaaatca cgcccaggcc aatgacaatc atcacgataa tcgtggtgac
     3241 tttgatcatc gcgaaccaga actcgatttc gccgtacaac cgcaccgccg ccagattcgc
     3301 caacgccacc agcgccactg cgatcaatgc gggtatccac tgcgccatct ccgggaacca
     3361 gaactggaca taaacgccaa tggcggtgat ttctgagatc cccaccgcca tccacataaa
     3421 ccaataagac caggcggtga gataaccaaa gaacgggctc atataacgat gcgcataaac
     3481 ggcgaacgaa ccggtaaccg gttcgaggaa caacatttcg cccattgaac gcatgatgaa
     3541 aaagacgaat agcccggcga tgatatagac caacaatacg gatggcccgg cccatttcaa
     3601 ggtactggcg gcccccataa acaggccgac gccaatggtg ccccccaggg cgatgagttc
     3661 gatatgtcga gcttccagcc cacgctgtag ctctggtttg ttatctgcca taaatcctcg
     3721 tgttgtgttt gcatgctttc cggtgttacc ggttatcgtt atgggtacat cgagtgttgc
     3781 gaatgttttc gtaattcagg agaaatggca aataaagcat taaaaatttg aatgctttgt
     3841 gtaataaaaa agcagacagg cgacggagtg accactccgt cgctttacaa agagaggaaa
     3901 atcagaggtt gccggtgtag tgccagcgta aataacgcag caaacgaagc tgacgcttaa
     3961 tgcggctcgg ctgcgaaagc aggcggtaga gccactccag tcccagcgtt tgccagattt
     4021 tcggtgcgcg ttttacgtga ccggtgaaaa catcgtaagt cccgccaacg cccatataca
     4081 gcgcatctgg atgcaccaga cggcagtcgc gcatgaagat ctcctgcttt ggcgatccca
     4141 tcgcaacggt gacgatttgc gctccgctgg catgaatgcg ttcaaacagc gcctgacgct
     4201 gctcgggttt aaaataacca tcctgactgc caacgatatt cacattccat tggttgcgca
     4261 gtttagcttc agtttgcgcc agcacttcag gtttaccgcc cacaagaaat accggcgtcc
     4321 cttctttgcc tgcgcgcgcc atcagctctt cccagagatc ggcaccggca acgcgggaaa
     4381 cctgcgcctg cgggtacttt ttacgtactg aacgtacaac gctgatgcca tccgcatatt
     4441 taaattcggc agcgttaatt aactccctga cctcggcgtt atcttcaatg gtcagcattt
     4501 tttcggcatt aatggcaacc agcgttccct gcttaagctg cccgtcagca aacagataat
     4561 cgagggcgtg ctgcatatca cgccaaccaa tcaactgtaa gccacgcagc gtataggtcg
     4621 gtgccgtggt gttgttattc attgttatcc ttcaacctgc gtccggagcg atgattttgt
     4681 acgtttatga atgagtccgg cgctttcaaa aagccagtac aacagttttg cgatcatcag
     4741 acatgcgccg aagaccacga taaaaaagac tacgcgtgag acaaacgaat ccagcccttc
     4801 acgtgccagc acgatcatat tgaagatcgc cccaaagcag aaactgtgca atatcgcagc
     4861 cttatagcga ttagtctcgc ggttgcccag ctcatacagc cagtcgaacc atttgatgat
     4921 cagtccaacc acaatcgccc cgagtgggat gaacaacgcg ccgcccatca ccaccagtga
     4981 gcctataagc gtaggcgaga tcgccagtcc ggagtggtta ttcagcactt cccaggtaaa
     5041 gtagttggct gagttcagca ccatactcgg gcgacccggc cacagccagg aagggataaa
     5101 gacatagaaa tcgcggacaa ttggagccag gccctggaag tcgatgttgt cgtagttctg
     5161 caacagcaac gccagattct cccatggcga gaaggtgtcg cgagtgagat agagaaacgt
     5221 atagaacgct tcatcgccgc tcacattcat tccatagcgt tttagcgcca gccagaacat
     5281 gcccacaatc cccagcacgc ccgccgccgc cagcatccac aacgaaatcc agccgcgaat
     5341 aatgccaata aacaggaaga tagcgaatgc gatgatgata ttggcgcgag tgccgccgac
     5401 aatcatataa gtcagcaagc caaaggcgac cgtgctgacg aggaaaaaca gccacgcttt
     5461 gctatcctgg cgcagaaagt agaccaccag catcgccggg atgaaaaagt aaaagaagcg
     5521 ttttaacgcc acgccggaga cttcactgga gaagatctga ctgtaggagt taagccggaa
     5581 cagcaaaaag ccgttgtgca tgaagaagat gccgacgctt accagcgcga tgcccatcag
     5641 gatcacccac gtaagattgg tctccacgcg gttcatggta aacagcggac ggcgcggtac
     5701 atcagcaacg cgtttgcgta ggcgggtttt gtaggtgaca tagtaaaccg cgtagaagca
     5761 gcccgcagaa agcaacgcct gcaacaagat ttctggcggc gcgacaccaa catcaaagcg
     5821 aaataccagc acgctggtca gcgggaagcc gaagaaaaag gtgagcaaaa acagcaatga
     5881 aaagaagaca ttgaagttaa agcgcacacg gcgaaactca aaccaggtca gcgtggcaat
     5941 aaacagcgtg cagagcagcc agacaacaaa caggccactg aattgcagca gactcatgcg
     6001 acctccccgg cggcaatcgc caacgcccgc tgccagcctt gtagatagtt agggctaaag
     6061 aaggcaatgg tgtttttatc caccgacgcc aactggcgct gcgcttcacg cacaatatcc
     6121 tcgttgagat cgtcagtagt aaacagcacc ggcaaatgct gttccgtcat atcctgccag
     6181 aacggatttt cccggttaag cacacaagga atgcccgcct gaatcagtaa gcaaagcgta
     6241 ccaatgccct gctggcgggc aaaaataaag taaccaagat cgcactgacg aagtagcgcc
     6301 agataggcgt caaattccag tttttcgctc aaaatttgca gattttcttc gctgaataac
     6361 tccagccccg cctgacgaac ttcctcaatg tacgcttcgt tattaggcgg atatcccatc
     6421 ggcaccacca cttttaccgt atcgccaaat tgctgatgaa cggcgcacaa ggcagcaata
     6481 tggtcattgc tgcggtcacc ggagttcccc accagaatgg tcattttccc ttcacgttgc
     6541 cgatcgttcg ccatcgtatt gagagaaggg tccatccgcg tcgggaagta cagcagttcg
     6601 ccccgcacct ttggatgcgt tttggcaaaa aagctcaaat cgccgcgggt ggcaaataca
     6661 cagccgactc gcttttgcgc caggcgacgt agtgggtaaa aaagcttata tctcaagcca
     6721 ctggaaagct catacaggtc tgccccccag atatgccaga aaaactggct gggcttaatg
     6781 ccaccactca gcagagccag ccacagtgtg ggattgaact gaccgtggaa gaaaaaacgc
     6841 tgctgacggt tagcttttgc tttcgcgatg accgcttccg ccagcgattt tttcccaggg
     6901 aaaaattgca cagaaagcgc cggacagcta tcacttaagc cgtcgtcctt gccaacaacc
     6961 ataaactcgc gcgcgtgctc gctcgtcgca gccagcgcgt cattgaaaaa ccgcaaaacg
     7021 gttcggttat ggtgagggat atccgatccc agtacgtgaa tcagtacagt catgcccgcc
     7081 tacgccagag taaaaacacg ccacaacaaa gagaaaaata gacgatataa gttgccatat
     7141 atgcctgcgc cgcgcccagt gcaccatgcg cagggattag ccagtgggca aataccatca
     7201 ataaagtgaa ctggctgatt tccgccagaa tataaaaccg caatgacgct ttggcgatca
     7261 ccagataacc aaagacataa gcgcccactt ttaacacatc acccactaac tgccaggcaa
     7321 agagatcgcg catagcggta aatttattcg acaacagcag ccagatagca aaatcacgca
     7381 gcagccagac ggtaaaactc gccgccgcca ctgccggtaa gacgaatttc agcgatttaa
     7441 ccacttcccg ggtgatatcg cgcttttccg ttagccgcga caacgtgggc agcaaatata
     7501 cgctgaacga tgccgtaata aattgcaggt aggcatcgga aatactgctc accccttgcc
     7561 agatccccac ttcatcccag ctatactgcg ccgcgagcag tttacgcatc atgatgtaag
     7621 caacaggcaa ggtcaccgac gtaatcaacg ccatgagcgt aaatttgctc aactgccctg
     7681 ccagaccgtt atcccagctg ggtttcagat agcttaacgg gatgacacca cgtttgatca
     7741 acatgatggc ggcaggaatt accaccagcg cgggaatcag cgccagaccc agcaacgccc
     7801 cttcataacc gcccaaacgg taactgacgt agtacgcgag aacgccaatc aagctgccga
     7861 caatcagcga taacgcatta cctgcggcat cgcgaaagcc tttcatcagc gccagtaaca
     7921 ggttgcccca ggcgatcccc atttgcacca gcgccactaa acgcaccagc ccctgatagt
     7981 cggtattacc aaacaatccc tggctgattg gcgcagctgc cagcacaaaa accagcgcca
     8041 tcagcgtaga gaaaccaagt accatcgctg atgaagtgcc gaccacgcgg cgcagctgtt
     8101 gcggattatc atggtactgg gcaacgtatt tggttacacc gttaaagatg ccagctccgg
     8161 caagcacgcc gagcacggta atcaactggc ggaaatttgc cgccagccca agccccgccg
     8221 gaccaaatga cacagccagc aacttaccga ccagtaaccc ggcaccaatc ttgaccagtg
     8281 tactggccgc cgtccacaag gacgcttttg ccaacgacat atcaggagaa gtagttcaac
     8341 aaagtcgcaa ttaccgtacg ctgattgacg ggcgacaggt tgtagaacag cggcaggcgc
     8401 agcaggcgct cgctctcttt ggtggtgtag cgatcttcac cgtggaactc accaaagcgt
     8461 tcccccgcag ggcaaccgtg cagcggaatg taatggaaca ccgccatgat ttccgcttct
     8521 ttcagaaagt taatcaacgc gctccggtca tcaatatccc gcagtttaat gtagaacata
     8581 tgcgcgttct gcacgcagcc atcgggaatc gacggcagct cgatacgccc ggctttcgcc
     8641 agaggcgcta acgcatcgta gtagttttgc cacagcgcca gacgttgctg gttgatacga
     8701 tccgctgctt ccagttgcgc ccacaggtac gcagcttgca gatcggacat caaatagctg
     8761 gagccaatat cgcgccaggt atatttatcg acctgaccac ggaagaactg gctgcggtta
     8821 gtgccctttt cacggatgat ctcggctcgt tcgattaacg ctttatcgtt aatcagcgtc
     8881 gcgccgcctt caccacccgc cgtatagttt ttggtttcat ggaagctaaa gcagccaata
     8941 tgaccaatgg ttcccagtgc acgccctttg taagtggata tcacgccctg agcggcatct
     9001 tctaccacaa acagattatg ctttttcgcc aacgccataa tggtgtccat ttcgcaggcc
     9061 acacccgcgt aatggaccgg cacgataacg cgcgttttgt cggtgatcgc cgcttcaatc
     9121 agcgtttcgt cgatgttcat ggtgtccggg cgaacatcca caaaaacgat ttttgcgcca
     9181 cgcagcacaa aggcattggc ggtggagaca aaggtgtagc tcggcatgat cacttcatcg
     9241 ccaggctgga tatcgagcag cagcgccgcc atctccagcg aagcggtgca ggacggcgtc
     9301 agtaacactt tggcgctacc aaaacgttgc tccagccact gctggcagcg acgggtaaaa
     9361 ccgccatcgc cacacagttt gccgctaccc attgccgact gcatatagtc gagttcggtt
     9421 cccaccaccg gcggtgcgtt aaatggaatc atgtgatcac ctgtataacc agtacgcggt
     9481 gctttctaca ttcgcaccac tttgtatgta tcgtttaagc gcggcggtgt tgcccatttg
     9541 ggtcgccacc cgcaaagttg ttttaccgcg agcatacgcc cagtttagcg ccgtttgcat
     9601 cagctcagca cctacaccgc gtccagccag caggccaatt cgcgcatctg tcgcattgag
     9661 ttcccgtaaa gagacataac cgcgaatacc gccggacgcc gcacgtaaaa tcagacattg
     9721 atgatcaaag gtgccgcgca cggcattttc aatccactgt gcataaaagc gtctgctggc
     9781 gtcaggcgca taccacggcg cacgaaaacg gctttgcgca aatgcggcgc tggctaactg
     9841 acgtaatgcg ggaatatcgg tctcttgtgc cactacagca ccactatcac tgacattgtt
     9901 cacgggtagt gccaaatcaa cttcaccttc taccagggag aatcccagct gttgcagggc
     9961 atccagttca cccgtatttg atgccgcaat tttggcctgc acccgtgacc acggcgctaa
    10021 cacgtctggc gtcaggagcg gtgcttcaga cgtaatgcgc acgatggcgc tgttaacacc
    10081 aaagaaggcg ttttcccagg ttagtggctc aatactggcg cggacgggca cgaagtaact
    10141 ccagcagata ttggccgtag ccagttttcg ctaatgaact ggcagcacgc ttcacaccct
    10201 cgtcatcgag ccagccgtta cgccaggcga tctcttccag gcaggcaatc ttaaagccct
    10261 ggcgtttttc tactgtctgc acaaaggtac tggcttcaat caggctgtcg tgagtgccag
    10321 tgtccagcca ggcaaatccg cgcccgagca gttcaacggt caggttgccc gcctcgaggt
    10381 acatctggtt gatggaggta atctccagtt caccacgctc cgacggcttc acctgctttg
    10441 cgtactccac gactttactg tcgtagaaat aaagcccggt caccgcccag tttgacttcg
    10501 gctgttttgg cttttcttcc agcgagatag cgcggaaatt gtcgtcaaac tccaccacgc
    10561 caaagcgttc cgggtccatc acctgatagc caaataccgt cgccccttcc gtgcgcgccg
    10621 caacatgacg cagcttcgga ctgaagccct gaccgaagaa gatgttatcg cccagcacca
    10681 gacaagaagg ttcaccgttg aggaaggttt caccgatgat aaaggcctgc gccagaccgt
    10741 cagggctggg ctgttcggcg tattccagct ggataccgaa ctcactacca tcgcccagca
    10801 ggcgctggaa ataaccttta tcttccggcg tagtgatgat gagaatttcg cggataccgg
    10861 ccagcatcag caccgacagc ggatagtaaa tcattggctt atcgtaaatc ggcaacagtt
    10921 gcttcgatac gccgcgcgta atcggatgca atcgggtacc ggaaccgccc gccaggataa
    10981 tacctttcat gtttgcctcc gctgaaaatt agcctttcag acctaaacgc tcgccctgat
    11041 agctgccgtc ctgcacctgc ttccaccagc tttcattagc cagataccac tgcaccgttt
    11101 tacgcattcc actttcaaag gtttcctgtg gcagccaacc aagttcacgg gcaattttcg
    11161 aagcatcaat ggcataacgc agatcatgcc ccggacggtc agcgacaaag gtgatcaagt
    11221 cacgatagtg cgccacgccg tgcggcttgt tcggagccag ttcttccagc agctcgcaaa
    11281 tggtttccac aacatcgaga ttcttacgct cgttgtgacc accaatatta taggtttcac
    11341 cgactttccc ggtggtcgcc acgcaataca gcgcgcgggc gtgatcttcc acatacagcc
    11401 agtcacggat ttgctgcccg ttgccatata ccggcagcgg tttacccgcc agcgcgttga
    11461 ggatcatcag cgggatcagt ttttccggaa agtggtaagg accgtagtta ttcgagcagt
    11521 tggtgatcag cgtcggcaga ccgtaggtcc gcagccaggc gcgcaccaga tggtcgctgc
    11581 tggcttttga cgcggaataa gggctgctcg gcgcatacgg cgtggtttcg gtgaagaaat
    11641 catccgtcga gtgcaggtca ccatatactt cgtcggtgga gatatgatga aaacggaacg
    11701 ctgatttttt atcttccgtc agcgtattcc agtaagcccg cgccgcttca agcaatgtat
    11761 aagtcccgac aatgttggtt tcaataaacg ctgccgggcc gtcaatagaa cggtcaacat
    11821 ggctttcggc tgccagatgc atgacacagt ctggctgatg ctcagtgaat acgcgtgcca
    11881 gttctgcccg atcgcagata tcaactttct caaaggcaaa gtgctcgctt tgcgcgaccg
    11941 gtgccagcga catcaggttt ccggcgtagg tcagcttatc gaccactacc accgcatcgc
    12001 tcgtttcgtt gatgatataa cgcaccagcg ccgagccaat aaacccggca ccacctgtta
    12061 tcagaatttt tctcatcagc gccagactcc tttggcatcg acgacatact gctgatggac
    12121 attgtcgcca ttgataactt tgaactgact atgatcgacc agcatcacca gcacgtctgc
    12181 cgttgccagc gcctcgtcaa gctgcgccag agtacaaagc ccggtcagtt ttttcggcaa
    12241 ctggtggatg ttaggctcaa caaccagcgt ttcgccgctg tgccactggg cgatcagttc
    12301 agcgatttcc atcgccgggc tttcgcgcag gtcatcaata ttcggtttaa acgccagacc
    12361 aaagcaggcg attttcagtt cactggcgcg tttatcggta gcagcaaggc agtcggcaac
    12421 cgccgctttc acctgatcga taacccagaa cggtttgtga tcgttaactt cgcgcgcggt
    12481 acggataagc cgcgcctgct gggggttctg tgccacgata aaccacggat caacagcaat
    12541 gcagtgaccg cccacgccag ggccaggctg aagaatatta acgcgagggt gacgattcgc
    12601 caggcgaatc agttcccaga cgttaatccc ctgatcggca caaatcagcg acaattcatt
    12661 agcaaaagcg atattcacat cgcggaagct gttttcggtg agcttacaca tttccgccgt
    12721 ccgcgagtta gtgacgacac actcaccttc gaggaaaatt ttgtacagtt cgctggcgcg
    12781 ggtcgaacaa accggcgtca taccaccaat cacgcgatcg tttttaatca gctcgaccat
    12841 aacctggccc ggtaacacgc gttccgggca gtaagcaatg ttgacgtccg cctgctcgcc
    12901 cacctgctgt gggaaagtga gatccggacg catctctgct aaccattctg ccatcttctc
    12961 ggttgacccc accggcgagg tggattcaag gatcaccagc gcgccttttt tcagcactgg
    13021 cgcaatggag cgagcagccg attcaacgta ggtcatatct ggctcatgat cgcctttaaa
    13081 cggcgtaggt acagcaatca gccaggcatc cgcttcaact ggcgtcgtgc tcgctcgtaa
    13141 aaaaccgcct tctacggcag tttttactac gctcgccaaa tcaggttcga cgatatggat
    13201 ttcgccacga ttgatggtat caaccgcgtg ttggttgata tcgacaccaa tcacctgttt
    13261 ttgccgtgag gcaaacgcag ctgctgtagg cagcccgatg taacccagtc cgataacaga
    13321 aatggtcgca aaactcatag tgatatccga ttatttttta acgcttccag aatgcgagag
    13381 catgcctgac catcaccata cgggttatgg gcgcggctca tagcttgata ttcgttttcg
    13441 tcttttaaaa gacgcgtcac ttcctcgaca attcgctgct tatccgtgcc taccagacgc
    13501 accgtacccg ccgtcaccgc ttccggacgc tcagtggtat cgcgcatcac cagcaccggt
    13561 ttccccagcg aaggcgcttc ttcctgaatg ccgcctgagt cggtcaaaat cagccaggcg
    13621 tggttcatca gccagacaaa cggtaaatac tcctggggat cgatcagaat gacatttttc
    13681 acatgcccca gaatgcgatt gaccggctct ctgacgttcg ggttgagatg caccggatag
    13741 acaatctgga tgtcctggtg cgtggtggcg atgtctgcca gcgcgtggca gatttcttca
    13801 aagccacgac cgaaactttc acgcctgtga ccggtcacca gaatcatctt tttatcgggg
    13861 tcgataaacg ggtaatttgc cgccagttct gaacgcagcg tgtcgctgct catcacctga
    13921 tcacgcaccc ataacagtgc atcaatgact gtattaccag taatgaagat tcggctatcc
    13981 gcaacgtttt cacgcagcaa gttttgccgg gaagtttcgg ttggagagaa gtgatacatc
    14041 gccagatgcc cggtcaatgt acggttagcc tcttccggcc acggcgaata gagatcgccc
    14101 gtgcgtaaac cagcctcaac gtgaccaaca ggaatacgct gataaaacgc cgccaggctg
    14161 gttgccagcg tcgttgttgt gtcgccgtga accagcacga cgtctggttt gaactcggca
    14221 agaataggtt ttagcccttc cagaatccga caggttatct ctgtcaggcc ctgtcctggc
    14281 tgcattatgt tgagatcgta gtcaggtaca atggaaaaga gtttcagcac ctgatcgagc
    14341 atctcccgat gctgcgcagt gacgcaaact ttagcctcaa aaaaaggatc ttttgccaac
    14401 gcatgcacca acggagccat cttgatggct tccgggcgcg taccaaatac agtcagtact
    14461 ttcacatcga ttctcttcga ataagcggcg agcgcctttg cgctcgccgc agcagtgttg
    14521 ctatttcgag caacggcggg ttaatgcgac accagccccg atcagccccc cgacaatgcc
    14581 ccacataatc atcaggaagg cacgacgtgg gctatcgcgt tttaccggtt cttccggcgt
    14641 acgcaaatag cgataggtct gaaaacgcgg atccagagtt ggaccaacat tcagggtgtt
    14701 taacatggcc cgattctgat catagtcgag atcaaaggcc ggaccgacgg cctgtaaatt
    14761 ttccagtcga gcctggagca ttggacgccc aagcaggaac atttctgaat caggtaattc
    14821 ctcggcaggc acatctgtcg cactgcgcga aatattatgc tgctcagcaa ttttcagcgc
    14881 ctgctcaatg ctgttcatcc ggcggtcgta gatggctttc gccacctctt cctgacgctt
    14941 cacctgagct ttcatctgga tggtgcgtgc cgcccatgcg cctttcagct catcattcag
    15001 atggctggct gcacgctggc tggcaaaagc aacatactga cgtaacaggt tattagcgtc
    15061 aggcgcggtt tcggcaataa gcttcacgct gtcattgacc gcgcgggtaa agtctccggg
    15121 gataaactgg atgttgttaa tcatttcatc cagcaacgcc gcatcggctt tgctgttgcc
    15181 caccatccgc tgtttgtaat agtcggtttg cagccagaac tctctgcggg tatcccacga
    15241 ggccagttgc ataacaaact ctttataggc ttcgtccatg accgatggtt gatcggcaga
    15301 agccatgttt gaacggacat ccaggttacg caaaaattgc tgctgcgagt aatatccccc
    15361 cagcatattc accgttggac gatcggtaat cgccgtcgag ctccactcct gacgagcaaa
    15421 aaaagtatac gccagcgcga ttaacgcaaa cgccagcccc atgccaataa tccatagctt
    15481 cccagcccac aaggtacgaa acaacccacg aatatccagt tcattttcag cgtcttcggc
    15541 cggtttccca ggcattggtt gtgtcatcac atcctcattt atttggttaa attggggctg
    15601 ccaccacgat ttctacgcag tctgcgtttt acgcgcttaa taaagcgagc aactttccag
    15661 gcacgcttaa tgcaatatcc atagaggaag aatgctagca aaaagagcac cagcatgacc
    15721 cactccggga caaaatgaga atattctgcc agcacgccaa tggaagcgag cagtgctgcg
    15781 gcaagggtaa tcagcacaaa cgcctgacgg gaagtaaacc cggcacgcat gatcaaatgg
    15841 tgaatatgct gacggtcagg agagaatggg ctcatgcctt tacgcaggcg acggtacata
    15901 atcgccacca tatccattag cggaatggcg attatccaca aagcggtaac cgggctgatg
    15961 ggatgggttt tgccctgggt cgtttcgagc aggatccaga taacggtaaa accaatcagc
    16021 gtactgcccg catcacccat aaagactttg tagcggcgac ccaggatacc aaggttaagc
    16081 atgatgtatg gcaggatggc ggcgatcatc gcaaagcacc agattgcgag gctggtttgc
    16141 ccgtcgaacc acaaaatcat accgattgct gcaaacgaga cgcaggacaa cccgcccagc
    16201 aagccatcaa tgccatcaac catgttgaac gcattaatgg ccgcccagac ggcaaatagc
    16261 gtcaggaagt aaccaaacgg tccgagcacc atctcccagg agccaaagat ataacccagg
    16321 ctactgagat aaagtttgcc gaacaccatc ataacaatgc caacagcggc ctgtatggtg
    16381 gcacggattt ttacgctgat atcaaaacgg tcatccagcg cgccaatgaa aacaagcaca
    16441 ccggcacaag cgagatagag agatgcatgc ggaatatagt aatcgacaat tccgaacgtg
    16501 aagcaaatcc ctgcgtaaac cgaaatcccc ccaacgagag gtatcaatcc ctggtgacgt
    16561 ttgcggaagt ttggtttatc cactaaaccg actttttttg ccaccttacg ggcaaaaaac
    16621 agaaacagtg tcgtgaataa aaaaatactg atgagatcag tactcactgt cagtaaattc
    16681 acaatgcatg ctctcagaga aaattattag cagaagtata accacgaaga cctttattca
    16741 gaagggaaag tccgttccga acccaccagt cctgtgcaaa aattaaccat ttgatatgca
    16801 aattgcataa catgccagca aattccagga attttcctaa catcgccagc gcggcattaa
    16861 gattacagat ataaaagcaa aacgccacgt aaacacgtgg cgtttttggc ataagacaaa
    16921 tttatgagcg tttcatcatt tcgaagaaat cgtcattggt cttggtcatg gccagtttat
    16981 taatgaggaa ttccattgca tcgatttcgc ccatcgggtg aatgattttg cgcaggatcc
    17041 acattttctg cagttcttcc tgagtcgtga gcagctcttc tttacgggta ccggaacggt
    17101 tgtagtcgat agccgggaag acgcgttttt cagcgatctt acgagagagg tgcagttcca
    17161 tgttgcctgt acctttaaac tcttcgtaaa taacttcgtc cattttagaa ccggtatcga
    17221 taagcgccgt cgcgataatg gtcaggctgc cgccctcttc cacgttacgc gccgcaccga
    17281 agaagcgttt cggacgatga agggcattgg cgtccacacc accggtcaac actttacctg
    17341 acgccggaac aacagtgttg taagcgcgcg ccagacgagt gatggagtcg agcagaatga
    17401 taacgtcttt cttgtgctca accaggcgtt tggccttctc gatcaccatt tccgcaacct
    17461 gaacgtggcg agatgcgggt tcgtcaaagg tagaagcaac aacttcacct tttaccagac
    17521 gctgcatctc ggttacttct tccggacgtt cgtcgatcag cagaaccatc agcacacaat
    17581 ccgggtggtt gtaagcaatg ctctgagcaa tgttctgcag cagcatagtt ttaccggctt
    17641 tcggcggtgc cacaatcaga ccacgctgac cacgaccgat aggtgatgcc agatccagta
    17701 cgcgagcggt taaatcttca gtagaaccgt taccacgttc catacgcaga cgagagtttg
    17761 cgtgcagcgg ggttaagttc tcaaagagga ttttgttgcg ggcgttttca ggtttgtcga
    17821 agttaacttc gttaactttc agcagcgcaa aatagcgttc accttctttc ggcgggcgaa
    17881 tcttaccaga gatggtatca ccagtgcgga ggttgaaacg gcggatttgg ctaggggaaa
    17941 cgtagatgtc atcaggaccg gcgaggtagg agctgtctgc ggaacggagg aaaccaaatc
    18001 catcctgcaa tatctccagt acgccatcac caaagatatc ttcgccactc tttgcgtgct
    18061 gcttcaggat ggcaaaaata atgtcctgct tacgcatacg agccaggttt tccagcccca
    18121 tattttcgcc gagagtgatc agctcagaaa ccggcgtatt ctttaattcg gtaagattca
    18181 tagtggtgtg agttcttaaa cttggggtaa atctcgaact taatgttgtg aatggtatgg
    18241 caggatcatc catgcctgtt tacggtcatc gactcatgtc ttttcgctgc ctggtcacag
    18301 gagagtacgc agaactgaaa cgacaagacg gattgagtga cgagccagaa atctgttcac
    18361 ttcgcattta agataaaacg ggaagcgttg gaaattacaa gattcaaact taataaggta
    18421 tgtttaatac gaagtcaaca ctaagttagc atgactcacg ccgggcgtcc agtttttagc
    18481 gacggggcac ccgaacatga aattccctta cgccaggtta gcgtcgagga actctttcaa
    18541 ctgaccttta gacagtgcac ccactttggt tgccgccact tcaccgtttt tgaacagcag
    18601 cagagtcggg ataccacgga tgccatattt cggcgcagtg ccagggtttt gatcgatgtt
    18661 cagttttgca acggtcagtt tgccctgata ttcgtcagcg atttcatcca gaatcggggc
    18721 gatcattttg cacggaccgc accactctgc ccagaaatcg acgaggatcg ccccgtccgc
    18781 tttgagtaca tccgtgtcaa aactgtcgtc agtcaggtga ataattttat cgctcatata
    18841 taactccaca ggaataagcc tggcgtgttg gtttcgttgt tggtgtaaca ttaaccaact
    18901 aaaggttgac tttatttcac cggatacgct ttcgtaaagc aatagtaagc tgatattcta
    18961 ccacactatg agcaaaacac atttaacaga acagaagttt tccgacttcg ccctgcatcc
    19021 gaaggttgta gaagcccttg aaaaaaaagg gtttcataac tgtacgccca ttcaggcact
    19081 ggcccttccg ctgacgctgg cgggtcgtga cgtagccggg caggcgcaaa ccggtaccgg
    19141 gaaaacgatg gcgtttctta cgtcaacgtt tcattatctt ctctctcatc ctgcgattgc
    19201 cgatcgcaag gtgaatcaac cacgtgcctt aattatggca ccgacgcgtg aacttgccgt
    19261 gcagatccat gccgacgcag aaccgctggc ggaagctacc ggcctgaagc tgggtctggc
    19321 ttacggtggt gatggctacg acaaacagct gaaagtgctg gaaagcggcg ttgacattct
    19381 gattggcact acggggcgtt taattgacta cgccaagcag aaccacatta acctcggtgc
    19441 cattcaggtg gtggtactgg acgaagccga tcgcatgtac gatctgggct ttattaaaga
    19501 tatccgctgg ctgttccgcc gtatgccgcc tgcaaaccag cgcctcaaca tgctgttctc
    19561 cgccacgctt tcgtaccggg tacgtgaact ggcgttcgag cagatgaaca atgccgaata
    19621 tattgaagtg gaaccggaac agaaaacggg ccaccgtatt aaagaagagc ttttctaccc
    19681 ttctaacgaa gaaaaaatgc gtttgctgca aacgctgatc gaagaagagt ggccagacag
    19741 agcgattatt ttcgccaata ccaaataccg ttgtgaagag atctggggcc acctggcggc
    19801 agatggtcat cgtgtcggtt tgttgacagg cgatgtcgcg cagaaaaaac gtctgcgtat
    19861 tcttgatgaa tttacccgtg gcgatctgga tattctggtt gccaccgacg ttgccgcgcg
    19921 tggtttgcat attccggcag tgacgcacgt ctttaactac gatttacccg atgactgtga
    19981 agattacgtt caccgtattg gtcgtacagg tcgcgcaggc gcaagcggtc actctatcag
    20041 cctggcgtgt gaagagtatg cattgaattt gcctgctatt gagacctata ttggtcactc
    20101 aattccggta agcaaataca atccggacgc attgatgacc gatctgccaa aaccgctgcg
    20161 cctcacgcgc ccacgtacag gcaatggtcc gcgtcgtact ggcgctccgc gtaatcgtcg
    20221 tcgttcaggt taaataaaat gtcatactca aaatcattca aactgtatca agatggcaag
    20281 agaatgaatc ccgatgagca tactgtagta agtgattcag gtgaaagaat gcagccaaca
    20341 cagagacaga ttgaaggatg aagagtatgg gttccacctc gtcgctgtat gcagccattg
    20401 atctcggttc gaatagtttt catatgctgg ttgtgcgcga ggtggctgga agcatccaga
    20461 cgctgacgcg aataaaacgc aaagtgcgtc tggctgctgg cctgaatagc gaaaatgccc
    20521 tgtctaatga agcaatggag cgcggttggc aatgtctgcg cctgtttgct gaacgtctgc
    20581 aagatatccc tccctcgcaa attcgcgttg tcgctacggc gacattacgt cttgccgtca
    20641 atgcgggtga ttttattgcc aaagcgcagg aaatcctcgg ttgtccggta caggtgatca
    20701 gcggtgaaga ggaagcacgt ctgatttatc agggcgttgc tcacacaact ggcggtgccg
    20761 atcagcgcct ggtggtggat ataggcggtg ccagtactga actggtaacc ggcacgggtg
    20821 cacaaaccac ctcgttgttc agcctgtcga tgggctgcgt cacctggctg gaacgctatt
    20881 ttgccgatcg taatctgggg caggaaaatt ttgatgctgc agaaaaagcg gcacgcgaag
    20941 tgttacgtcc ggttgccgat gaattacggt atcacggctg gaaagtgtgc gttggtgctt
    21001 ccggcaccgt gcaggcgtta caggaaatca tgatggcaca ggggatggat gaacgcatta
    21061 ccctggaaaa gttgcagcaa ttgaaacagc gagccattca ttgcggtcgg ctggaggaac
    21121 tggagattga cgggctgacg ctggaacgtg cgttagtgtt cccgagtggt ctggcgatcc
    21181 tgatcgccat ttttaccgaa ctgaatattc agtgtatgac cctggcgggc ggtgcgctgc
    21241 gtgaaggcct ggtctacggc atgttgcatc tggccgtcga gcaggatatt cgcagccgta
    21301 cgctgcgtaa tattcagcgc cgctttatga tcgatattga tcaggcgcag cgcgtagcca
    21361 aagttgcggc taacttcttc gatcaggtgg aaaaggaatg gcatcttgaa gcaataagcc
    21421 gcgatttgct catcagcgcc tgtcagcttc atgaaatcgg cctgagcgtt gactttaaac
    21481 aagcgccgca acacgctgct tatctggtgc gtaatctgga tcttcccggt tttacccccg
    21541 cacagaaaaa attgctggcg acgctactgc tcaaccagac taatccggtc gatctctcat
    21601 cgctgcatca gcaaaatgcc gtaccgccgc gcgtcgcaga acaactctgc cgtttactac
    21661 gcctggctat catttttgcc agccgtcgcc gtgacgatct cgtgccagag atgacattac
    21721 aggctaacca tgaactgttg accttgacgc ttccgcaagg ttggctaacc caacatccgc
    21781 tgggtaaaga gattattgct caggaaagcc agtggcagag ctatgtacac tggccgctgg
    21841 aagtgcatta atgagtaagt gccggatgcg atgctgacgc atcttttccg gccttgatta
    21901 tttccctcgt tttgccgcca tcatcgcttt cagattcgcc agatggcttt gccctttctg
    21961 catccgttct tcggcgctga ccactttgcg ctcctgttcc caaatcagat catcctgcgg
    22021 cagctccagc aaaaagcggc tcggctccgg gcgcaccagc tcgccgtact gacggcgttc
    22081 tttacacagc gtaaaggtca attccttctg ggcgcgggta atgccgacat aggccagccg
    22141 ccgctcctca tcgatattat cttcatcgat gctgctctgg tgcggcaaaa acccttcttc
    22201 cataccgacc atgtagacat aaggaaactc cagccctttt gacgcgtgca gagtcatcag
    22261 ttgcacctga tccagctctt cttcactctc accacgctcc atcatgtcgc gcaaagtaaa
    22321 gcgcgtcacc acctgggtga gcgtcatcgg ctcatccagt tcgcggcctt ccagcatctc
    22381 cgtcatccag ctaaacagtt ggttgacgtt cttcatgcgc atttcggcgg ctttcgggct
    22441 gggcgatgtt tcgtacagcc aggattcata atccatgccg tggatcagat cgcgcaccgc
    22501 ggcaatcggc tcccgctccg ccagacgctg gatttctgcc aaccagtgag taaagcgggt
    22561 caatgcttca taaccacgtc cgctaagcgt ctgactcagg cccatatcaa agctggcggt
    22621 aaacatgctt ttattgcgcg tcatcgccca ctcacccagc tttttcagcg tcgccgggcc
    22681 aatctctctc ttcggcgtgt taacgatacg cagaaatgcg ctgtcatcgt ccgggttagt
    22741 cagcacgcgc agataagcca gcaagtcctt gatttcagga cgagagaaaa acgacgtacc
    22801 accagaaatt ttgtacggga tgcggttttg catcaggaat ttttcaaaca ctcgcgactg
    22861 atggttaccg cgataaagaa tagcgtaatc tttgtactgc gttttattga cgaagtgatg
    22921 cgcaatcagt tcaccagtga cgcgctcggc ttcatgttct tcgttattcg cgcttaaaac
    22981 tttaagctct gtgccgtaac ccagttcgga gaacagacgc ttctcaaaga cgtgcgggtt
    23041 attggcgatc aggatgttcg ccgctttcaa aatacgtccg gaagagcggt agttctgctc
    23101 aagcttaatc accttcagcg ccggaaaatc ctgactcagc agcaccaggt tttgtggacg
    23161 tgcgccgcgc caggagtaga tcgactggtc gtcatcgccc accacggtaa agcgcgcgcg
    23221 gctgcccacc agcagtttca ccagttcata ctggctggtg ttggtgtcct gatactcatc
    23281 caccagcaga tagcgaattt tgttctgcca gcgctcgcgg acttcttcat tgcgttgcag
    23341 cagcaacgtc ggcaataaaa tcagatcatc gaagtcgaga acattgcacg ctttcaggtg
    23401 tgcatcatac agcccataac aatgggcaaa aatacggtcc cgctcgccaa tcgcactggc
    23461 tgccgcctgg gacggtgttt tgagatcgtt cttccagtta gagatggtcg aaatcaactg
    23521 ttgcaggaga actttgtcat cttcaatcag cccttcggtc aactctttga gcaacgccag
    23581 ctgatcggta tcgtcaaaca gcgaaaagtt cgccttcatc cccagcgccg catactcacg
    23641 cttgatgata tccagcccca gcgtgtggaa ggttgagatc atcagcccac gcgcctcttt
    23701 gcgacccagc gtctgtccga cacgctcttt catctcgcgc gccgccttat tggtaaaggt
    23761 caccgccgcg atatgtcgcg cctgataacc gcaaccgcgg atcagatggg cgattttatt
    23821 ggtgatcaca cgagttttac cggaacccgc gcccgccagc accaggcagg ggccggtaac
    23881 gaattcgaca gcttgttgtt ggccggggtt tagacgcata ggtgtattgc tcaatcttcg
    23941 aacgggggga ggattgtagc agaatcgcat ttgaatgtga ccgcgtgtaa gccgctaacc
    24001 ggttggctgc ggatgtttaa atcagagaga gaagaccgaa accggagttc atcgtgaatc
    24061 cattagactt agaaaatatc gggtcgtaac gttgtgttat tactttttca gagtttacta
    24121 aggagaggtg tactcgtggc gattaatttc agtcctaagg ttggtgaaat actggaatgc
    24181 aactttggta actatcctgt tagccagaat ggacagttca gcaccacgta ttacgatggc
    24241 cgcattcctc ctgaaatgat aaaaaacagg ttagttgtcg tgctcaacgg aaaaattaat
    24301 ggcaacgcat gtatcgtcgt tccattatca accactcgcg atcatgacaa gttaaatcgt
    24361 ggaatgcatg ttgaaatagc cagtaatgtt atcaatggcc tgcaattctt tgatcagcag
    24421 attcgttggg ccaaagccga cctggtacaa caagtcagtc gaaaccgatt aaatagagca
    24481 aggacttaca gagggtatct taatcaatgt ttgccacatg agttggttgc agacatacag
    24541 agggcggtca tcaagtcaat caatgccata tcgctcataa attgacaata aaatcaccat
    24601 ccccgataat gactttgtag cccatagagg ccctaagttt tctccccttg agggacctgc
    24661 gaagcccgga ttttccgggc ttttgccata tatgtaccag cccctccctg actgacaatt
    24721 acgttagcat ctgcggatca tgctaaaatc gccgcctgac aattattcac tcaaggcacg
    24781 atcatggcaa aaacagcagc agcactgcat atccttgtaa aagaagagaa actggctctg
    24841 gatcttctcg agcagattaa gaacggtgcc gatttcggca agctggcgaa gaaacactcc
    24901 atttgcccat caggcaaacg cggcggtgat ttaggtgaat tccgccaggg tcagatggtt
    24961 ccggcgttcg ataaagttgt gttctcttgt ccggtactgg agccgaccgg cccgctgcac
    25021 acccagttcg gttaccacat cattaaggtg ctgtaccgca actaatagca aggccttctc
    25081 caggagaagg ccttgagtgt tttctccctc tccctgtggg agagggtcgg ggtgagggca
    25141 tcagcgcgca cttaacccgc aacagcaata cgtttcatat ctgtcatata gccgcgcagt
    25201 ttcttaccta cctgctcaat cgcatggctg cgaatcgctt cgttcacatc acgcagttgc
    25261 gcgttatcta ccgcgccttc cggaatagct ttacccaagt cgcccggttg cagctctgcc
    25321 ataaacggtt tcagcaacgg cacacaagcg taagagaaca gatagttacc gtactcagcg
    25381 gtatcagaga taaccacgtt catttcgtac agacgcttac gggcgatggt gttggcaatc
    25441 agcggcagct cgtgcagtga ttcataatat gcagactctt caatgatgcc ggaatcgacc
    25501 atggtttcga acgccagttc aacgcccgct ttcaccatcg cgatcatcag tacgccttta
    25561 tcgaagtact cctgctcgcc gattttgcct tcatactgcg gcgcggtttc aaacgcggtt
    25621 ttgccggtct cttcacgcca ggtcagcagt ttcttatcat cgttggccca gtccgccatc
    25681 ataccggaag agaattcgcc ggagatgatg tcgtccatat gtttctggaa cagcggtgcc
    25741 atgatctctt tcagctgttc agaaagcgca taagcacgca gtttcgccgg gttagagaga
    25801 cggtccatca tcagggtgat gccgccctgc ttcagcgctt cggtgatggt ttcccaaccg
    25861 aactgaatta gtttttctgc gtatgccgga tcggtgccct cttccaccag cttgtcgaag
    25921 cacagcagcg agccagcctg caacataccg cacaggatgg tttgctcacc catcaggtca
    25981 gatttcactt ccgcaacgaa ggacgattcc agcacgcccg cacggtgacc accggttgct
    26041 gccgcccatg ctttagcgat cgccatacct tcgcctttcg gatcgttttc cgggtgaacg
    26101 gcaatcagcg tcggtacgcc gaatccacgt ttgtactctt cacgcacttc ggtgccaggg
    26161 catttcggcg caaccatgac tacggtgata tctttacgga tctgctcgcc cacttcgacg
    26221 atgttgaaac cgtgcgagta acccagcgcc gcgccgtctt tcatcagtgg ctgtacggtg
    26281 cgcactacat cagagtgctg cttgtccggc gtcaggttaa ccaccagatc cgcctgcggg
    26341 atcagttctt cgtaagtacc aactttaaaa ccgttttcgg tcgctttacg ccaggacgcg
    26401 cgcttctcgg caatcgcttc tttacgcaga gcgtaggaga tatcgagacc agaatcacgc
    26461 atgttcaggc cctggttcag cccctgtgcg ccacagccga cgataaccac ttttttaccc
    26521 tgaaggtagc tcgcgccgtc ggcgaattcg tcgcgcccca taaagcgaca tttgcccagc
    26581 tgtgccagct gctggcgcag gttcagtgta ttgaagtagt tagccatggt gattcctcgt
    26641 gatgttgtgc ttcttattgt tcggttcgcg tttgcgagat tgaattcact atatgacagg
    26701 aaatttattg cggaaattga tatattcaca acgtcacatt gcaatttctg caacgtcaac
    26761 attgagggct gcgccattgg atttacgcga tctgaaaacc ttcctgcatc tggcggaaag
    26821 ccgccatttt ggccgcagcg cgcgggcgat gcacgttagc ccgtccacgc tctcacggca
    26881 gattcagcgc ctggaagacg atctcggtca ggcattattt gtgcgcgata accggacggt
    26941 gacgctgacc gaagcgggcg aagagttacg cgttttcgcc cagcaaacat tgttgcagta
    27001 tcagcaactg cgccacacca tcgatcagca agggccgtcg ctctctggcg agttacatat
    27061 tttctgctcg gtgaccgccg cttacagcca tctaccgccg attctcgacc gcttccgcgc
    27121 ggaacacccg tcggtggaga ttaaacttac tactggtgat gcggcagatg cgatggaaaa
    27181 ggtggtcact ggtgaagcgg atctggcgat tgcgggtaaa ccggaaacct tgcccggcgc
    27241 agtggcgttt tcgatgctgg agaatctggc agtagtgctg attgcccccg cgctgccctg
    27301 cccggtgcgt aatcaggtgt cggtagagaa gccggactgg tcaacggtgc cgtttatcat
    27361 ggccgatcag gggccggtac gccgccgcat tgaactgtgg tttcggcgta acaaaatcag
    27421 taacccgatg atttacgcca cggttggcgg gcatgaagcg atggtatcga tggtggcgct
    27481 cggctgtggc gtggcgctgt tgccggaagt ggtgctggaa aacagcccgg aaccggtgcg
    27541 taaccgcgtg atgatactag agcgaagcga tgagaaaacg ccgtttgagc tgggcgtgtg
    27601 tgcgcagaaa aagcggctgc atgagccgct gattgaagcg ttctggaaga ttttaccgaa
    27661 ccactaaccc gccaaaaaga acctgaacgc cgggttattg gtttcgtcgt ggcaatcgta
    27721 gcccagttca ttcagccggg tttcgaaatc cggttcatgg tcgccaagct cgaacgccgc
    27781 cagtacgcgc ccgtagtcgg taccgtgact gcgataatgg aacagagaaa tattccagtg
    27841 cgtacccagc gtgttgagga agcgcagcag cgcacccggc gattccggaa attcgaagct
    27901 gtagagacgt tcctgcaacg gatgcgatgg acgcccgcca accatatagc gcacatgtag
    27961 cttcgccatt tcatcgtcgg agagatcaac cacgctgtag ccgccgtcgt tgagcatctg
    28021 caaaatttct ttgcgctctt cgaggccgcg gcttaagcgc acgccgacaa agatgcaggc
    28081 gtttttggca tcggcaaagc ggtagttgaa ttcggtgacc gaacgtccgc caagcaactg
    28141 gcagaatttg aggaagctac ctttctcttc cggaatggtt accgccagca acgcttcacg
    28201 ctgttcaccc agctcacaac gttccgagac gtagcgcagg ccgtgaaagt tcacgttagc
    28261 accggaaaga atatgcgcca gccgttcgcc gcgaatgttg tgctgggcga tatatttttt
    28321 cattcccgcc agcgccagcg cgccagaggg ttccgccacc gcgcgcacat cttcgaataa
    28381 atccttcatc gccgcacaga tcgcatcact atcgacggtg atgatgtcgt cgagatactc
    28441 ctggcataaa cggaaggttt cgtcaccgat gcgttttacc gctacgcctt cagcaaatag
    28501 ccctacgcgc ggcagatcaa ccggatgacc cgcatccagc gctgctttca ggcaggcgga
    28561 gtcttccgct tctacggcga tcactttgat ttgtggcatc agttgtttga tcagcaccgc
    28621 aacgcccgcc gccagaccgc cgccgccgac tggcacaaat acgcggtcga gatgggcgtc
    28681 ctgctggagc agttccagcg ccagcgtgcc ttgcccggca atcaccatcg gatggtcgaa
    28741 cggcggcacc caggtgaacc cctgctgctg tgacagttcg atcgctttag ctttcgcttc
    28801 gtcaaagttc gcgccatgaa gcagcacttc gccgccgaat ccacgtaccg catcgacttt
    28861 gatgtcggcg gtggcggttg gcataacgat cagggccttc acgcctaacc gcgcagaaga
    28921 aaacgcgacg ccctgcgcgt ggttacccgc agaagcagtg atcacgccgt gcgctttttg
    28981 ttcttccgtc aggcccgcca tcatggcgta tgcgccgcgc agcttaaagc tgtgcactgg
    29041 ctggcgatct tcgcgcttca ccagaatgac gttatcaaga cgcgacgaca gtttttccat
    29101 tttttgtagc ggcgtaacct gcgccgcctc gtaaaccggc gcgcgcagca ctgctcttaa
    29161 atattcggca ccttccggag caccggacag gggttgcgag tcagccatta ttaccccccc
    29221 agtttcgatt tatcgcgcac cgcacctttg tcggcgctgg ttgccaggct ggcgtaggcg
    29281 cgcagggcaa aggaaacctg acgttcacgg tttttcggcg tccaggcttt gtcaccccga
    29341 gcttcctgcg cttcacgacg cgccgccagt tcggcatcgc ttacctgtaa ctgaatgcca
    29401 cggttcggga tgtcgatagc gatcaggtca ccatcttcaa tcagaccaat gctgccgccg
    29461 cttgccgctt ccggtgagac gtggccgatg gaaagaccag aggtgccgcc agagaaacga
    29521 ccgtcggtga tcagcgcaca ggctttaccg agacccattg atttcaggaa gctggttggg
    29581 tagagcattt cctgcatgcc agggccgcct ttcgggcctt catagcggat caccaccaca
    29641 tcgcccgcga caactttgcc accgaggatc gcttctaccg cgtcgtcctg gctttcgtac
    29701 actttcgccg ggccggtgaa tttgaggatg ctgtcatcga cgcccgcggt tttaacgatg
    29761 cagccgtttt ctgcgaaatt accgtagagc accgccaggc cgccgtcttt gctgtaggcg
    29821 tgttccagcg agcggataca gccattggcg cgatcgtcat cgagagaatc ccaacggcaa
    29881 tcctgcgaga atgcctgtgt ggtacgaatg cctgcaggac ctgcgcggaa catatttttt
    29941 accgcatcat cctgggtcag cataacgtcg tattgttcca gcgtttgcgg caacgtcagg
    30001 ccaagtacgt ttttcacatc acggttcagt aaccccgcgc gatccagttc gccgagaata
    30061 ccgataacac caccagcacg gtgaacatct tccatatggt atttctgggt gctcggcgca
    30121 actttacaca gctgtggaac cttgcgggaa agcttatcga tatcactcat ggtgaagtcg
    30181 atttccgctt cctgcgccgc cgccagcagg tgaagtacgg tgttagtcga tccccccatc
    30241 gcgatatcca gcgtcatggc gttttcaaac gccgccttac tggcgatatt acgcggcagt
    30301 gcactttcgt cgttttgctc gtaataacgt ttggtcaatt caacaatgcg tttaccagca
    30361 ttaaggaaca gctgcttacg gtcggcgtgg gttgccagca gcgagccgtt gcccggctgc
    30421 gacaggccca gcgcttcggt caggcagttc attgagttag cggtaaacat cccggagcag
    30481 gaaccgcagg ttgggcaggc ggaacgttca acctgatcgc tctgggagtc agaaactttc
    30541 gggtctgcgc cctggatcat cgcatcaacc agatcgagtt tgattatctg atcggacagt
    30601 ttggttttcc cggcctccat cgggccgccg gaaacaaaga tcaccggaat attcaggcgc
    30661 agggaagcca tcagcatccc cggggtgatt ttgtcgcagt tagagatgca gaccatggcg
    30721 tcggcgcagt gggcgttgac catatactca acggaatcag cgatcagttc gcgagatggc
    30781 agtgaataaa gcatcccccc gtggcccatg gcaatcccat catccaccgc aatggtgttg
    30841 aactctttgg caacgccgcc agccgcttca atttgttcgg cgaccagttt accgagatcg
    30901 cgcagatgga cgtgacccgg tacaaattgg gtgaacgagt tcacaaccgc gataatcggc
    30961 ttaccgaaat cggcgtcggt cattccggtg gcgcgccaca gcgcacgagc acccgccata
    31021 ttacgaccat gagtggtggt ggcggaacgg tacttaggca tactttattt actcccagtg
    31081 tctgtctcgt aaatgggacg gtgcgtgccg tcccattttt tgtatttatt gattaacttg
    31141 atctaaccag ccccatttat cttcggtttc gccagtgaag aggccgaaga aggcttgctg
    31201 aatgcgtttg gtaaccgggc cacaacggcc ttcgccaacc tgaataccgt ctacgctgcg
    31261 cactggcgtg atttctgccg ccgtaccgga cataaacact tcatccgcca ggtacaggga
    31321 ttcgcgcgac agcacctgct cacgtacttc aattcccagc tctttcgcca gtttgatgat
    31381 ggcatcacgg gtaatacccg gcagcgcgga ggaggtgaac ggtggggtga acagcacacc
    31441 atctttcact tcaaacaggt tttcgcctgc gccttcagag atataaccgt tcacatccag
    31501 cgcgatacct tcctgataac cgtggcggcg cgcttcgcta cccaccagca gggaagagag
    31561 gtagttacca ccggcttttg ccgccgtcgg gatggtgttt ggtgctgcgc ggttccagga
    31621 ggaaaccatc gcatcgatcc cctgctccag cgcttctgcg cccagatacg ctccccacgg
    31681 gaaagcagcg ataatcacgt cggttgagta tcccgctggc gggtttactc ccatgccaac
    31741 atcaccgacg aagatcagcg gacggatata ggcgctggtg agattatttt tgcggatcac
    31801 gtcacgacaa gcttccatca gctcatcaat gctctgcgaa accgggaagc gatagatttt
    31861 ggcggagtca tgcagacgct gcatatgctc acgatggcgg aatacaaccg gtcctttgtg
    31921 cgagtcgtag caacggatgc cttcaaaaac cgaagtgcca tagtgcagcg cgtgcgacat
    31981 cacatgcacc ttcgcgtctt cccagcgaac catctcccca ttgaaccaaa tgtaatcagc
    32041 tttcttcgtg gtcattttta tattcctttt gcgctcaggc gcggatttgt tgtgatgtgg
    32101 ttgtgctctg gcagatggca acgtgtgcga cgtccaccag tttatttaac tgactaaaca
    32161 gtaagtcgac cgaccgtggg ctggcaacgg tcaattcgat atttatattt tgtgcatcgc
    32221 tggcggcggc catattcatt gagcagacgt ggaaaccacg atgacgcacc acgcgtaaaa
    32281 cacgttctaa ggtttccgga ttgaagcgag ccgatacatt gacctgatgt tgcatcatga
    32341 taatttctcc aacatttctg aattactggc accaggcggc accagcggcc agacgttctc
    32401 aagttcgtcg attgagacat gaagcaggta tggcccatca ctgttcagca tggtgtcgag
    32461 tgccgcttca acctggtctt tacgggtgat gtgttggcca gggatgccga aggcgctggc
    32521 taacatgagg aaatcggggt tatcagtaag ggtggtttcg ctgtatcgtt cctgaaaaaa
    32581 cagttgctgc cattgtcgaa ccatccctaa ccgttggtta tcgagtaaga cgattttcaa
    32641 cggtaactgc ttgcgtttta cggtgcccag ctcttgcaca ttcatcatga aagagccgtc
    32701 accggagata cagacgacag tatcgttcgg tcgtgcgact tgtgcgccaa ccgccgctgg
    32761 taaaccgaaa cccatggtgc ctaagccgct ggaggtaatg aaattttccg ggcgagtgtg
    32821 tgcgatgtgc tgcgcggccc acatctggtg ctgccccaca tctgtggtca cgacgcaatc
    32881 cgcaggttta cgatccgaca gttgttttaa caacaacggc gcgtagatag cgtcaccggg
    32941 atggtcgtaa cgccaggtat gttcatcacg cagctgcgcg cagtgttgct gccagtcatt
    33001 gatatttaac ggctgctgta atgctggtaa cagagcattt aaatcacctt gtaatgccac
    33061 atgtgcctga cgcagcttgt tcatttctgc cgggtcgata tccatatgga taacactggc
    33121 gtgtggtgcg aaggtgttca gtttgccggt cacccggtca tcaaaacgtg cacccacggc
    33181 gatcagcagg tcacactcct gcaccgcgaa gttcgccgct ttggtgccat gcattcccag
    33241 catgcccagg tagtacggat aatctgcttc aactgcgccc agccctttca gcgtgcaggt
    33301 ggcaggcatt tttgtggtag cgagaaattc tcgtaaagca ggaactgcct gcgccatacc
    33361 cacgccacca ccaacgtaca gcatcggttt ttgcgctttt gccagcatct ggcgcgcttg
    33421 ctcaacttcg gcatggggga aagtcacttc gttttcaacg gtggtgaacc acggttccag
    33481 atcgccgctg gctaactgga tatcttttgg gatatcgacc agaaccggac caggacgacc
    33541 tgagctggca acgtcgaatg cttcagccat aatgcgcggc aactcttcca gcgactgcac
    33601 cagaaagctg tgcttggtac aggctaacga caatcccagg acatccactt cctgaaatgc
    33661 gtccgtgccg ataaacggtg cggacacttg accggtgatg gcaacaacag ggatagaatc
    33721 taacagtgcg tccgcaagcc cggttatcag gttggttgcg cccggaccag acgtggcgat
    33781 acatacgcca gttttgccgg tagcacgggc ataaccgata gccgccattg ccgcaccctg
    33841 ctcatgtcgg cacagcaagt gctccacgcc gccgtcatac aatgcatcgt aaaccggcat
    33901 aattgcgcca cccggataac cgaaaacggt gttcacaccc tgtgcccgca acgcatgtac
    33961 cacccactgt gcgccattca tagttagttc cccgtcctga atcttgagaa acagaatttt
    34021 gtgctgttat tcattgtctg ctcctcggtt atgtttttaa ggtcaaaaaa aacccccgga
    34081 cctttcggtg cgggggtctt agttcgttaa ggcttgatct ctaagccttt cctcgtccaa
    34141 gtgcagcccc gcacggtggg ataataatca ccaccacgct aatcacgacc aggctaatca
    34201 ctcgtagaag ggctgtcatt ttgtcttttc ttgcatcttg ttcgaaggaa tgcctaaaga
    34261 gttaccatag attttgcgaa tagcacaaga tattttttgg ccgattttta tgatgcgttt
    34321 aataatttca atagaacttg ttgtttttta aaaagaaaat agtgagtgaa aaaaattcaa
    34381 tttcagtatg aatgttgttc ccttcctcgt agttcatcct ttgtccgctg catttttgcg
    34441 aacatcattc caccgccaga acaactcaac ttcacttgca caaataaaca ggaatcagcc
    34501 actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata atagtgactc
    34561 aaggagggct tatgtcactg tcaattgttc atacccgcgc agccctggga gtaaatgcgc
    34621 ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg atggtgggct
    34681 taccagaaac aactgtaaaa gaagctcgcg atcgcgtgcg cagcgccatt atcaatagcg
    34741 gatatgaata tccggcgaaa aaaatcacca tcaacctggc tccagccgat ctgccaaaag
    34801 aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca gaacagctta
    34861 cagccaataa gttagatgaa tatgaattag tcggagaact ggcgcttaca ggcgctctgc
    34921 gtggcgttcc cggcgcaatc tccagtgcaa ctgaagctat taagtcgggc agaaaaatta
    34981 tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga tgcctgatag
    35041 ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gcacgctctc gaacgcccga
    35101 aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt gtcggtcagg
    35161 agcaaggaaa gcgaggactg gaaattaccg ccgctggcgg gcacaacctt ttactgattg
    35221 ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg ttgccagatt
    35281 taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat gctgaatcag
    35341 tacaaaaaca atggcggcag cgcccgttcc gctcaccgca tcacagtgcg tcgttaactg
    35401 cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg cataacggcg
    35461 tgctttttct tgatgagtta cctgaatttg aaaggcgtac actggatgcc ttgcgagagc
    35521 cgattgaatc cgggcagatc catctttcac gcacgcgagc aaaaataacc tatccagccc
    35581 gtttccagct tgtcgcggcg atgaatccca gccctaccgg acattatcag ggaaaccata
    35641 accgctgcac accagagcaa acgctgcgtt atctcaaccg gctctcgggg ccctttctcg
    35701 accgcttcga tctctcactg gagatcccat taccaccccc cggcattttg agtaaaacgg
    35761 tagtgccggg agaaagcagc gccaccgtta aacaacgcgt aatggccgcc agagagcgcc
    35821 aatttaagcg gcagaataaa ctgaatgcct ggctggatag tccggaaata cgccaattct
    35881 gcaaacttga gagcgaagat gcgatgtggt tggaaggaac actgatccat ctggggttat
    35941 cgattcgtgc ctggcagcgg ttattgaaag ttgcacgaac cattgctgat attgatcagt
    36001 ctgacattat cacacgtcag catttgcagg aggcagttag ctatcgtgcg attgaccgtt
    36061 tgctcatcca tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc tttttattaa
    36121 tcatcagaat cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc ggaaagggtg
    36181 tgaaaacgtt ttggacgctt aatacgcgtc atatacttgg accacacgcg ttctgcttct
    36241 gtcactggct cacgttcgcc acggcatact gctacgaaga gtttctcttc ctcggtaacc
    36301 ggctcgcgtt tgccaagatc caactcattg aaggcataac catgacgctc aagcagttgt
    36361 gcctctttga tggtgaaatc accatgacga gagaatccac gtggataatg tttattgtcg
    36421 aaatatcgat tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa ttctttgacc
    36481 gagctagtta tggcgcggag tattagttac gcttgacaga gtgtaaaaca aaacatttaa
    36541 atcataacga caaataattt tgcggagagc actgtggata cggaattgtt aaaaactttc
    36601 ctggaagtta gccgaacgcg tcactttggt cgagcggctg aatcgctcta tctgacccag
    36661 tcagcagtga gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa ccttttcacc
    36721 cgccacagaa acaatatccg tttaaccgct gccggtgaaa aactactgcc ttatgcagaa
    36781 acgctcatga gcacctggca ggccgcccgt aaggaggtgg cgcatacctc acgacataac
    36841 gagttttcta tcggtgccag cgcctcgttg tgggaatgta tgcttaatca gtggctggga
    36901 cgcttgtatc aaaatcagga tgcccataca ggcttacagt tcgaagcgcg aattgcccaa
    36961 cggcagtctc tggtaaaaca gctgcatgaa cgccagcttg atcttcttat taccactgaa
    37021 gcgcccaaaa tggacgaact tagtagtcag ttgctgggat atttcacttt agcgctttat
    37081 accagtgccc cttcaaaact aaagggagat cttaattatc tgcgacttga gtgggggcca
    37141 gattttcaac agcatgaggc aggtttgatc ggtgctgacg aagtgcccat tctgacaacc
    37201 agttctgctg aactggcaca gcaacagatt gcgatgctta atggttgcac ctggctaccc
    37261 gtcagctggg cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac aacactttca
    37321 cggccgcttt atgccatatg gctgcaaaat agcgataaaa atgcgttgat tcgcgatctt
    37381 ttgaaaatta acgtgctgga tgaagtgtat taatatgaat ggctggcaag gatgccggta
    37441 gaaggattta cttcggagag ggttatttca gataaaaaaa atccttagct ttcgctaagg
    37501 atgatttctg gcaggggcgg agagactcga actcccaaca cccggttttg gagaccggtg
    37561 ctctaccaat tgaactacgc ccctaattag ggtggcggaa cggacgggac tcgaacccgc
    37621 gaccccctgc gtgacaggca ggtattctaa ccgactgaac taccgctcca ccgaattctt
    37681 ttacaaccac cggttttatg accggctt
//
LOCUS       DAZGXZ010000032        37535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000032 DAZGXZ010000000
VERSION     DAZGXZ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.032"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHKJF_001771"
     CDS             complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHKJF_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP0949142.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHKJF_001772"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHKJF_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0949143.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..3125)
                     /gene="yhdX"
                     /locus_tag="ACHKJF_001773"
     CDS             complement(1944..3125)
                     /gene="yhdX"
                     /locus_tag="ACHKJF_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0949144.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3193..4218)
                     /locus_tag="ACHKJF_001774"
     CDS             complement(3193..4218)
                     /locus_tag="ACHKJF_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0949145.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4649..4870)
                     /locus_tag="ACHKJF_001775"
     CDS             complement(4649..4870)
                     /locus_tag="ACHKJF_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949146.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNICMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5123..8227)
                     /gene="acrF"
                     /locus_tag="ACHKJF_001776"
     CDS             complement(5123..8227)
                     /gene="acrF"
                     /locus_tag="ACHKJF_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFP0949147.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8239..9396)
                     /gene="acrE"
                     /locus_tag="ACHKJF_001777"
     CDS             complement(8239..9396)
                     /gene="acrE"
                     /locus_tag="ACHKJF_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP0949148.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            9795..10457
                     /gene="acrS"
                     /locus_tag="ACHKJF_001778"
     CDS             9795..10457
                     /gene="acrS"
                     /locus_tag="ACHKJF_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP0949149.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10460..10639)
                     /gene="yhdU"
                     /locus_tag="ACHKJF_001779"
     CDS             complement(10460..10639)
                     /gene="yhdU"
                     /locus_tag="ACHKJF_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP0949150.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(10723..11607)
                     /gene="yhdJ"
                     /locus_tag="ACHKJF_001780"
     CDS             complement(10723..11607)
                     /gene="yhdJ"
                     /locus_tag="ACHKJF_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0949151.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDAASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            complement(11693..11989)
                     /gene="fis"
                     /locus_tag="ACHKJF_001781"
     CDS             complement(11693..11989)
                     /gene="fis"
                     /locus_tag="ACHKJF_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP0949152.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12015..12980)
                     /gene="dusB"
                     /locus_tag="ACHKJF_001782"
     CDS             complement(12015..12980)
                     /gene="dusB"
                     /locus_tag="ACHKJF_001782"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP0949153.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13309..14190)
                     /gene="prmA"
                     /locus_tag="ACHKJF_001783"
     CDS             complement(13309..14190)
                     /gene="prmA"
                     /locus_tag="ACHKJF_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP0949154.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14202..15653)
                     /gene="panF"
                     /locus_tag="ACHKJF_001784"
     CDS             complement(14202..15653)
                     /gene="panF"
                     /locus_tag="ACHKJF_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP0949155.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(15643..15885)
                     /gene="yhdT"
                     /locus_tag="ACHKJF_001785"
     CDS             complement(15643..15885)
                     /gene="yhdT"
                     /locus_tag="ACHKJF_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP0949156.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(15994..17343)
                     /gene="accC"
                     /locus_tag="ACHKJF_001786"
     CDS             complement(15994..17343)
                     /gene="accC"
                     /locus_tag="ACHKJF_001786"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP0949157.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17354..17824)
                     /gene="accB"
                     /locus_tag="ACHKJF_001787"
     CDS             complement(17354..17824)
                     /gene="accB"
                     /locus_tag="ACHKJF_001787"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP0949158.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(18802..19776)
                     /gene="yhdH"
                     /locus_tag="ACHKJF_001788"
     CDS             complement(18802..19776)
                     /gene="yhdH"
                     /locus_tag="ACHKJF_001788"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP0949159.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            19928..21868
                     /gene="csrD"
                     /locus_tag="ACHKJF_001789"
     CDS             19928..21868
                     /gene="csrD"
                     /locus_tag="ACHKJF_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP0949160.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            22173..23216
                     /gene="mreB"
                     /locus_tag="ACHKJF_001790"
     CDS             22173..23216
                     /gene="mreB"
                     /locus_tag="ACHKJF_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP0949161.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            23282..24385
                     /gene="mreC"
                     /locus_tag="ACHKJF_001791"
     CDS             23282..24385
                     /gene="mreC"
                     /locus_tag="ACHKJF_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP0949162.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            24385..24873
                     /gene="mreD"
                     /locus_tag="ACHKJF_001792"
     CDS             24385..24873
                     /gene="mreD"
                     /locus_tag="ACHKJF_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP0949163.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            24882..25475
                     /gene="yhdE"
                     /locus_tag="ACHKJF_001793"
     CDS             24882..25475
                     /gene="yhdE"
                     /locus_tag="ACHKJF_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP0949164.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            25465..26934
                     /gene="rng"
                     /locus_tag="ACHKJF_001794"
     CDS             25465..26934
                     /gene="rng"
                     /locus_tag="ACHKJF_001794"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP0949165.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            27002..30802
                     /gene="yhdP"
                     /locus_tag="ACHKJF_001795"
     CDS             27002..30802
                     /gene="yhdP"
                     /locus_tag="ACHKJF_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP0949166.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSMAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            30958..32403
                     /gene="tldD"
                     /locus_tag="ACHKJF_001796"
     CDS             30958..32403
                     /gene="tldD"
                     /locus_tag="ACHKJF_001796"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP0949167.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(32531..33460)
                     /gene="aaeR"
                     /locus_tag="ACHKJF_001797"
     CDS             complement(32531..33460)
                     /gene="aaeR"
                     /locus_tag="ACHKJF_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP0949168.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            33643..33846
                     /gene="aaeX"
                     /locus_tag="ACHKJF_001798"
     CDS             33643..33846
                     /gene="aaeX"
                     /locus_tag="ACHKJF_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP0949169.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            33854..34786
                     /gene="aaeA"
                     /locus_tag="ACHKJF_001799"
     CDS             33854..34786
                     /gene="aaeA"
                     /locus_tag="ACHKJF_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP0949170.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            34792..36759
                     /gene="aaeB"
                     /locus_tag="ACHKJF_001800"
     CDS             34792..36759
                     /gene="aaeB"
                     /locus_tag="ACHKJF_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP0949171.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            36851..37123
                     /gene="yhcO"
                     /locus_tag="ACHKJF_001801"
     CDS             36851..37123
                     /gene="yhcO"
                     /locus_tag="ACHKJF_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP0949172.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(37179..37442)
                     /gene="yhcN"
                     /locus_tag="ACHKJF_001802"
     CDS             complement(37179..37442)
                     /gene="yhcN"
                     /locus_tag="ACHKJF_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP0949173.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         9263 a         9462 c         9706 g         9104 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat attgcccttt aggcagcgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatattggtg
     1261 ccttctgcca taaacaaagg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccagcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt catggctatc
     2041 gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga aaccatatcg
     2101 ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata ctggctggtt
     2161 aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag cgtaaccggg
     2221 ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc ctggatccca
     2281 gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag cgttaaggct
     2341 gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag ggctgggaca
     2401 tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc aatgatcaac
     2461 actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta tgttttatta
     2521 aagcggaata gcccaacaga aaggactata gccataacaa cagccaggat aaacgcaata
     2581 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
     2641 tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
     2701 aagaagattt gcagcagcgg gggaatatta cggaagatct caatataaat tgtggatagt
     2761 tttcgtagca gccagttatc cgaaagtctc gccagaccga taaagaagcc taaaacagaa
     2821 gcgaacacaa tacacaatgc agaaaccagt agcgtattga gtaagccgac aataaaaacg
     2881 cgtccgtagg tgtcgccctg ctggtaatcg atcaaatgct ggacaatacc gaagccagcg
     2941 ccgcgatcca gaaaggcaaa acctgaagta atgccacgat tattgagatt cgttacagtg
     3001 ttgtgaaata accaaccaac aatgccgaca acagcaacaa cggcaaggat ctggaataac
     3061 caggcgcgaa ccgtagggtt ggcaaaagag agtgagcctt taacggttga gcggcgatga
     3121 gacatagaaa cctcggaaag cataacgctg aagagagcgc cgccgtagcg acgcccgtta
     3181 cctcacaccg acttaacgca cgggcggcgc gtactgaata ccgccgttat tccagagatt
     3241 attttgccca cgtttaattt tcagcgggct ttctgaacct acgttacgct cgaaaatttc
     3301 cgagtagtta cccacctgtt tgatgatgtt ataggcccat ttattatcca gcttcagatc
     3361 cttgccgtaa tcgccttctt tacccagcag atgtgccata tcaggcgttg ctggattagc
     3421 cgctttttca tcgacgttct gggaattgat gcccatctct tcagcattca gcatggcgaa
     3481 aagcgtccag cgtacaatcg agaaccattc atcatcgcca cgacgaacta ccggcccaag
     3541 cggttcttta gagataacct ccggtaagac aatccattca gctgggttgc ttaatttgat
     3601 gcgcagggca tacagttgcg actgatccga ggccagcgta tcgcagcgac cagattccag
     3661 tgccttcgct gattcgtcag agcgatcgaa agtcaccggt gtgtacttca tattgtttgc
     3721 cttgaaatag tcggcaacgt tgagttcggt atcagtaccc gcctgaatac agacggtagc
     3781 gccatccagt tctttcgcgc tttttagccc cgctttatcg tgcgtcagga agccaatgcc
     3841 gtcgtaataa gtgacgccgg taaatgccat tcccatcccg gcatcgcgag atgaagtcca
     3901 ggtcgtatta cgggagagca aatccacttc ccctgactgt aaagcggtga agcgttcttt
     3961 tgctgtgagt ggggtatatt tcactttcgt gtcgtcacca aatacagcag cggcaacacc
     4021 acgacaaaca tcaacatcaa tacctgaaaa cttaccgtct gcatcggcat aagagaaccc
     4081 aggtaatcca tcactgatcc cgcattgcac aaaacctttt ttctgaacgg catcaagcgt
     4141 cgcgccagca tgcgcctgat ttgcaacggc aagcagcacg ctggcggcag ccagtgtggc
     4201 tatcatcatc tttttcataa tgcttcctgt gaggcgaaaa ttatcgttat aggacgtttg
     4261 ggaaacgtct tttacctgtc tgtggccatc ctcttataag caaaagtagt gccagctttt
     4321 gcgcccgatt tcgcgtcgca ataagctgag tgtagacagg aaataataaa aacagcgccc
     4381 cgcaatggtg cgaaaatgaa acatgcgcca cattttggcg caaaatattt acgcgtgttt
     4441 tactaagcag aataatgatt gttagcgtga gtatatttct ggtgggaagt gtgaaagtta
     4501 aattgatgcg ttatttcgaa agcagcgaaa taacgcgata taacatgcta aatcagcaga
     4561 gaattagcca gcagaatata gcaatatttt atcttatgta aagatactgt atcgttaaac
     4621 agagcgcgaa actccgcgcc ctgttcagtt atttcgacag agcgatgatc cccatcgtca
     4681 ggccagcaac agccgctgcc ccgccagcaa tagccccagc cgtttcccag ttatcctgag
     4741 tcgtgccttt catacagata ttggtgtgaa ccagacgacc ctgatcgtca taaaccggaa
     4801 cgcagggcga atcgtgagca cagcccgcca gcaatgcggt gagcaatgcc acaggaatta
     4861 atcttttcat ggtgttacct cagtaataaa cgctaacgct taataagtta actctcttat
     4921 taacgagctg aatgccattt tatcaccgga ggttataaat cttgcggaat gaataatgac
     4981 aaattatgtg aattgtaaaa atagtgaagg gagcgggaac tatagaaagc ttttaaagag
     5041 tgagcagaag aatatcttat taatttttat gggtttcact ggaaataata aaggcacccg
     5101 aaagcgcctt tatgtttctg atttatcctt taaagcaacg gcggatcacc acaaagaaca
     5161 ccggtacgaa gaagattgcc agcaacgttg cagagaccat tcctcccatt accccgatac
     5221 ccacagcgtt ctgcgcgcca ctgccggcac cgttactgat agctagcggt aatacgccga
     5281 gaataaaggc gagagaggtc atcaggatag gacgcagacg catacgtact gccatcagtg
     5341 tcgcttcaac aacaccttta ccctctttct ccatgagatc tttagcgaac tcaacaatca
     5401 aaatagcgtt tttggccgac aagccaattg tcgttagcaa gcccaccata aagtagacgt
     5461 catttttttg attaaagagt gttgccgcca gtagcacgcc gacaatccct aacggcacta
     5521 ccaacataac cgagacagga attgaccagc tttcatagag tgcagcaagg cacaggaaaa
     5581 caaccacaaa ggaaattgct accagagcgg gagcctggtt tcccgataag cgttcctgat
     5641 acgacatacc cgtccagtca taaccaatgc ccgcaggtaa ttttgacgca aggttttcca
     5701 tcaacgccat ggcatcgccg gaactggttc ctggcgcggc ttccccctga atctccattg
     5761 acggcagacc gttgtagcgt tccagtcgcg gagagccata cacccaatgt gaagtggtaa
     5821 aggccgagaa tggcaccatt tcgccgttgg cgctgcggac ataaagttta tcgacatctt
     5881 ctggcagcat acggaatttg gcatccgcct gaacatacac ctttttcacg cgaccacggt
     5941 cgatgaagtc gttaacgtaa gtcccaccca acgccgttga aatggtctga ttgatgtcag
     6001 aaagtgaaac acctaatgcc tgcgcctttt cctggtcaac ttccagttta aactgcgcgg
     6061 tgtcttccag gccattagga cgcacgctga ctaagctggc aggatgttgc gccgccatgc
     6121 caagcaactg gttacgggcc tgggtcaggg catcgtgacc cagcccagcc tgatcaatta
     6181 actcaaagtc gaaacccgtt gccgtgccca gttcaacaat ggctggcata ttgaatggaa
     6241 tgacaaaacc gtcgcggatc ttgcccaatt ccattttggc acgatggatt accgcttccg
     6301 cactgttttc atcactatta cgctcttccc acggtttcag actgacgaag gccataccgg
     6361 cgttttgtgc ctggccgctg aagctaaagc cgttaaccgt aaagacactt tcaacgttcg
     6421 ctttctcgtt cttcagatag taatccgtca cttgatccaa cactttttgc gtccgctctt
     6481 gcgtcgcgcc agcgggtaac tgaatcatgg tcagaaagac accctgatcc tcttcaggta
     6541 agaaggaaga cggaagacgt aaaaacaaca ccaccattcc tgcaacaatc agcgcataga
     6601 tcagtaaata tcgtcctgtg gaaccgagga ttttgccgac gctgttggtg tagtggttaa
     6661 cgctatgatc gaaggtggta ttaaaccagc cgaagaaacc acccttattt tcgtgatgct
     6721 cagctgaggt gggtttgagc agtgttgcac ataacgcagg ggtaagaatc aatgccacca
     6781 gaacagaaag cgccattgcc gaaacgatgg tgatagagaa ctggcgataa attgccccag
     6841 tagaaccgcc gaagaatgcc atcggaataa ataccgctga cagcaccatc gcgataccca
     6901 ccagtgcgcc ctgaatttgc gacatcgatt tttccgtcgc ttcttttggc gggagcttat
     6961 cctccatcat cacgcgctcg acgttctcca ccaccactat cgcatcatcg acgagcagcc
     7021 cgatggcaag caccatcccg aacatcgtta gtgtgttgat ggagtaacca aaagcggcga
     7081 ggatggcaaa cgtccctaat aacaccacgg gtaccgcaat ggtggggatc agcgttgctc
     7141 gcatattctg caagaacaga tacatcacca ggaacaccag cataatggct tcgaacagcg
     7201 tttttaccac ttcgtgaata gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa
     7261 ccttcattcc ctgcgggaag aatggctgta attccgccag ttttgcctta atggctttcg
     7321 cggtatcgag agcattcgcg ccggttgcca gcttaatccc caggcccgcc gccggttttc
     7381 cgttgatacg agcgataacg ttatagtttt caccgccaag ttcaactcgt gcgacatctt
     7441 tcaggcgtac caccgagccg tcactgttta cgcgcagggt tactttgccg aattcttccg
     7501 gattttttaa ccgcgtctga gcaataattg aggcgttcaa ctgttgccct ggtaacgctg
     7561 gcgttccgcc caactgtccg gcagcgatct gatcgttctg taccttcaac tggttaatca
     7621 catcaaccgg tgtcagttta tatttgttta gcagatcggc gtccagccag atacgcatcg
     7681 catactgtgc gccgaaaagc tgtacgtcac cgacgccatt cagacggcta agcgtatctt
     7741 taacgttaga ggccacatag tccgagatat cgtcctgtgt ggtgtctggg ttatcagaga
     7801 caaagcccgc caccatcaaa tagctgctac tggacttttc aacactgatc ccctgctgct
     7861 gaacctcctg cggcagcaac ggcgtggcga gctggagttt gttctgcacc tgcacttgcg
     7921 cgatatcagg atcggtcccg gactggaagg taagggtaat tgtcacgcta ccggcggaat
     7981 cgctggtgga ggacatatac atcaggttat cgataccgtt catattctgt tcgataacct
     8041 gcgtcaccgt atcctgcacg gtctgcgcat cagcgcccgg atagtttgct gaaacagaaa
     8101 ccgcaggcgg tgcaatggtt ggatactgag cgacgggtaa ttgtaggatc gccagtgcgc
     8161 ccgccatcat cagaataatg gccagcaccc atgcaaatat cggtcgtcga ataaaaaagt
     8221 ttgccatgtc agattacctt acttcgatgc agtatctgcg ggggtatcgg tagtggcttt
     8281 aacctgctct cccggacgcg ctttttgcag gccgctgaca atgacttgat cgccagattt
     8341 cagaccttcg ctaatcaacc atttatcgcc aatcgcctga ctggcaacga cagggcgcgc
     8401 ttccacctga cttttatcgt taacaatcag cacggttgca tcaccacgcg gtgtgcggct
     8461 aacgccttgt tgcgggataa gaatggcgtc aggttggacg ccttcatcaa tccgtgcgcg
     8521 cacaaacata cccggcaaaa gcgtatgttg cgggttaggg aagacggcac gtagggttat
     8581 ggagccagtg ctttcatcaa cggtcacatc agagaattgc agcgtacctt tcaggggata
     8641 ggtttgaccg ttttccatga ccaactctac gttgctggtg gcgttttcct tatgcaaatt
     8701 tccttgctct acggattgct tcagcctcat aaaatcgttg ctggattggg tcacatcaac
     8761 gtagatagga tcgagctgct ggacagtcgc cagttcagtc gtttgcccat tagtgacaag
     8821 agcgccttcg gtcacggtcg atttgccgat acgtccgcta attggcgcag tcactttggt
     8881 ataagcgaga ttgatgcgag cgctttcgac tgtggctttt gcggcaatca cggcggcatc
     8941 ggcctggcga gcatcagcaa tggcctggtc gtactcctgc tggctgatgt atttcgtacc
     9001 cacgagcgga acgtaacgtt ttaccgtcaa atgcgcgatg gcggcggcgg cttcactttt
     9061 cgccagttcg cctttcgcgc tgtcataatt tgcctgatag gtcgcgggat cgatctggta
     9121 cagggactgg cctgcttgca catcgctgcc ttcagtgaaa ttgcgattca gtacgatccc
     9181 gctaacctgt gggcgaactt cggctatacg gtaagcattg gtgcggcctg gtaattcagt
     9241 cttaacttct aacggggccg tttttacaat atgaacggta acctgcggtt caccgacgtg
     9301 agctttctct tctcccttat cgttacaacc ggcgattaaa gccgcggaga tcagaataaa
     9361 ggagggcagg aggaaaaacc tggcatgttt cgtcattact attcctcaaa aaaccaaaag
     9421 cgcgttattt acccaaaaag gcaacgcgtt aactcgcaga aagaaaaata cagttcgcta
     9481 tcctacaaat tatcattcgt cgatgtaagg aatagttatg aatacaggcg tctcaaggca
     9541 cataaacaca aaaaaagatt aatattctac tgttttattt tgacgcgggt tgaaagaggc
     9601 ggaattaaaa catcgtaaat tgaaatatat attgatgtag tgaatgtatc ttaggtaaat
     9661 aatatatatt atttatttaa cgatttatag caactcaata ttagcctctc gtataaatac
     9721 acattaggtg atagattaac cttcgctatt ttctcactct gtgtcgaata tatttatttc
     9781 ctgaataatt aatcatggca aaaagaacca aagccgaagc tctgaagacc cggcaagaac
     9841 tgattgaaac tgccatcgcc cagtttgcgc agcatggcgt aagcaagacg acactcaacg
     9901 acattgccga cgctgctaac gttacgcgtg gcgctatcta ctggcacttc gaaaacaaga
     9961 ctcaactgtt taatgagatg tggttgcaac agccttcatt gcgagagtta atccaggatc
    10021 acttgacggc tgggttagag catgacccat ttcaacaatt gcgtgaaaaa ttgattgtcg
    10081 gcttgcaata tattgccaaa attccccgcc agcaggcgtt gctgaaaatc ttatatcaca
    10141 aatgtgaatt taatgatgag atgctggccg agggagtgat acgcgaaaag atgggcttca
    10201 atccgcagac tctccgcgaa gtattgcagg cgtgtcagca acaaggttgt gtagcaaata
    10261 acctcgattt agatgttgtg atgattatta ttaatggtgc cttcagcgga attgttcaaa
    10321 actggttaat gaatatggcg ggttatgatc tttataaaca agcccccgct ctggtcgata
    10381 acgtattaag aatgttcatg ccagatgaaa acataacgaa attaattcat caaacgaatg
    10441 aattaagtgt catgtaaatt cacattctgt cctgaaaatt cagttcgtca cagttaacca
    10501 gttttagtgg attaactgag ttcatattgc ggcatttgtc tgtttctgtt gccagcagtt
    10561 caatccactg catcagtaaa gtatcaaaca gaaaaacgaa gacagcgaaa acgacgagcc
    10621 accaatactt gcgaatcatt acctgagcct gtgtatgttt aaccaagtct ggcaggaaat
    10681 atacacgaaa tgaataatct gaaaaccagg cttacacttc ctttactttg caatgagatc
    10741 ggggtcaact tcgctaaccc gacatcgttt tgaccggttg cccgtctttc tttttttcac
    10801 tttcgccagt tcctccgcgg agtaatgtga cgcggcatcc agtcgtcgaa gccccatttt
    10861 gatgtactcg ctgttgatct caataccaat gaattttcgc ccgctggcga cggctacggc
    10921 accggtagta aagctaccgg caaacgggtc gagaacgata tcgcctgggt tggaagaggc
    10981 gagaataatg cgtttcagta aggcttccgg tttttgcgtc gggtggttct catattcatc
    11041 catcaaataa cgcacgcgcg gaaaatccca gacgttaccc ggtacttttt gatgattgta
    11101 tggctgtgga ggatttttgc gataatcgat caacgcgcgc tgcgatccgg ttttggcttc
    11161 taccagaata gcatcaccgt tgaatgtgta gttctttgcg tctttcacca tcatcaggat
    11221 gggttcgtac atggagccgt agtgtttttt cgcctgtact ccagaactgt catatgacca
    11281 gacgatgcga cttttgatgg taaaaagctt gcggcactgg agatcgataa agggcatgtt
    11341 ttccgtgctg ttcatgatgt acatgctgcc ctgctttttc agaacgcggt ggcactctgc
    11401 aatcacttca aacagccagt cgataaacag atcttctttc caggcttcga tcagaccatc
    11461 aaaattttta ccgatgttat atggtgggtc ggcaaagatc agatcgacac tttcggcggg
    11521 gatcttttta agttcggcaa gcgcatcacc gtgaataatg gtcttagctt cattaccaaa
    11581 ccgggtcggt tcacatcctg ttctcatggt cactcccttt gtgacaccta taaaaaaggc
    11641 gcttccccat gccgagtagc gcctttttaa tcaagcattt agctaacctg aattagttca
    11701 tgccgtattt tttcaatttt ttacgcagcg taccacggtt gatgcccatc atcagcgcag
    11761 cacgggtctg gttaccacgg gtgtattgca tcaccatgtc caacaggggc tgttctactt
    11821 cagccagtac cagctcatag aggtcattca catcctgacc attcagttga gcaaaatagt
    11881 tcttcagtgc ctgtttaacc gagtcacgca ggggtttttg ggttacctga tcctgagagt
    11941 taacggtaga aacggtcagt acgtcagaat ttacgcgttg ttcgaacata gttctgtcag
    12001 ctctttattt ctgtttacgc aaaattttcg aagtatgcct ccaacgcctc cagctgttcg
    12061 ctggcatcct caatggcgtt gaatgtgcgc cgaaactggt catttggagc gtgttcctgg
    12121 agataccagg aaacgtgttt acgtgcaatt cggtaccctt ttgccggacc ataaaagtca
    12181 tgcagttccc gaacgtgcgc gcaaagcaag cgcttaacct ctgccaaagg cagcgggggc
    12241 agcaactccc cagtgtccag ataatgctgg atttcccgaa agatccaggg tcttccctga
    12301 gctgcgcggc ctatcatcag ggcatccgcc cctgtatagt cgagcacagc tctggcttta
    12361 agcgggtcag taatgtcgcc attcgcgata accggaatgg aaactttctg cttaactgcc
    12421 cgaatactgt cgtactcagc ttctccattg aacaaacagg cgcgtgtacg gccatgaatg
    12481 gtcagagcct gaatgccaca gtcttcagcc agttgggcaa tctcttcgca gttacggtgt
    12541 tccggtgccc agccggtgcg aatcttcagg gtaacaggaa cgtccactgc attgacgacc
    12601 tcggtaagga tcgatttaac gacatccggg tactgcaaga gggctgaacc tgcgagcttg
    12661 cgattcactt ttttagccgg gcaacccata ttgatatcaa taatctgggc accgctttcc
    12721 acgttaatac gtgctgcatc tgccatttct ttcggatcgc taccagcaat ttgcacggtg
    12781 cgaataccgg gttcatcaat gtgcaccatc cgtaaacgag atttgtcgct ttcccaaacc
    12841 tgtgggttag aagacatcat ctcggatact gtcaatccgg ctcccatctc gtagcacaac
    12901 gtccgaaaag gtctgtctgt aatgccagcc atgggcgctg cgatcaggcg atttctgagc
    12961 tgatattgtc cgatgcgcat gagttaagaa atgaccatac tgtgactgca aggcggcgta
    13021 tattacgcat tttttgcacg agatgaaagg ccaaactttg accaatcctc tgagatggat
    13081 caaagaattg catttaaaat gagcgtggtg cgataattac tcataaaaat catcatatta
    13141 gaaaatagtg actaaaattt acactcaaag aaatttgagt aagttctcaa tttttcttta
    13201 tgaatgaaaa ttttggcacg caaatttgcg taaataatcg gcaaatttac gtgccttttg
    13261 tgagcttgct cgcacttcgc cccgcgtcac cctacggcga tgcgaaggtt aattcttacg
    13321 accggtaata cggcaccact cttctttttc cacgaccggg tccagtgcga agctgtcggc
    13381 ataagcttca caaacgctct ctgcctggct tgccagaata ccggaaaggc ccagcaaacc
    13441 gcctgaaacc ggcaggacgc tgattaacgg tgccagttca cgtaatgggc ctgcaaggat
    13501 gttagcgacc accacgtcgg ctttcatttc ttctggctga tctttcggta agtagagttc
    13561 cagacggtca gaaacgccat tacgttcggc gttgtcgcgg ctggcctgaa tcgcctgcgg
    13621 atcgatatca ataccaatgg cttttgctgc acccagtttc agcgccgcga tcgccagaat
    13681 gccggaacca cagccaaagt cgatgactgt tttaccggtt aaatcgaggc tgtcgagcca
    13741 ttgcaggcac agagaggtgg ttggatgggt acccgtacca aacgccagcc ctggatctaa
    13801 catcacgttg acggcgtttt cgtccggcac atcacgccag ctagggcaga tccacagtcg
    13861 ttcaccaaag cgcatcgggt ggaaattatc catccattcg cgctcccagt ctttatcttc
    13921 tagttgttcg attttatgcg cgaagcctgc gccgagcagc ggatggtttt ccagaatcgc
    13981 caccacgtcg ttcatatcgg tttcagcgtc aaacagacca atcacatcgg tgtcgcccca
    14041 caggcgcgtt tcgcccggca gcggctcaaa tactggcgta tcgtgggtat cctgaaaagt
    14101 gatagaaacg gcacccgctt ccatcagcgc atcgctaaga tcttccgcgt tcgcgccggt
    14161 ggtgttcagt ttcagttgga tccaaggcat ggcaaaactc tttatttatc agtagtcaaa
    14221 acggtagctt gcgggacgga tgtaccgaaa cggtttccga ccaggaaagc cagcaaactt
    14281 agtagtaacg agggcacgat agggtggaag cccaggtact gaatattcag cgtcgcgagt
    14341 acggcataca gcacgccgcc aacgatcatc gcacttagcg cgcctttggc gttggcgcgt
    14401 tcccagtaaa gacccagcac cagcggccac aggaaaacgg cttccagccc accgaaggcc
    14461 agcaaattca gccagatgat catttctggc ggcttccagg cggcaagcag cagcaacgcg
    14521 ccgagaacta acgtaattac cgccgacatc cgcttcagac gcgtctcgtt ttgcatttga
    14581 tccggacgga tattcagata gagatcttta atgatcgtag cggaactttg cagcaactgg
    14641 gcattgatcg tcgacatgat cgcagccata ggtgcagcca ggaagattcc ggcagcaaac
    14701 ggtggcagca cttttaccat taacgtcggg atcaccagat ccggtacggt gagatcgggg
    14761 atcactgcac gacctaacgc tccggccagg tgcataccga acatcaggat tgcgactaca
    14821 atcgtaccga tgatgatccc ccggtgtact gctttgctgt ctttataaga gatacagcgc
    14881 accgcagtat gcggcaggcc aatcacgcca aagcacacca gtacccagaa cgacgtcata
    14941 aaggcaggcg acagaatatc gtcagcgcct tgtggcgtaa ccagttgcgg atcgatggtt
    15001 tgcaaggtct gtactgcgtt gcttaagccg ccagcggcat gtaccacgcc aataagcagc
    15061 acaacggtgc caatcagcat cacaagccct tgcatggtgt cgttcagcac gctggcgcga
    15121 aagccaccaa aggcggtata taacgcaatg ctgataccaa aaatcagcag cccggtttca
    15181 taaggaatac ccgccgcggt ttccagcagg cgcgcaccgc cgataaactg cacggtcatc
    15241 gcaccaacga acgcaaccag caaactcaaa ctcgccagcc acaccagaag acgactctgg
    15301 tagcgggcaa acagcatatc gttcagcgtc actgcattgt agcggcgcgc aagaatcgca
    15361 aacttcttgc cgagaatacc gagtgaaagc cagactgcag gaagctgaat catcgccagc
    15421 aatacccagc ccagcccgta tttataagca gctcctggcc cgccgataaa cgaactggca
    15481 ctgatatagg tcgcggtgag cgtcatcgcc agcacaatac cgcccataga gcggctgccg
    15541 aggaaatact cattaaggaa ggtgccggtg ctccgtttac gcatcgcata aaccgagata
    15601 ccgaacacca ccaccagata ggcgaccagc ggtagaatta cttcaagctg catcgtcatc
    15661 ctccagtggg atatcgcgat agataaattt caccatcgcc cagcacagtc caataaacag
    15721 cagcggcgtc aggatgcagg ccatctcaaa ccagcgcgga aagccggtaa aaccgggggc
    15781 aacgccaggt aagtaagcgg ctactaacca taccgccaga tacaaaaggg tcagccccag
    15841 cgcccagcgc gcctctttat gggcctgaac aaaacgagtg tccatttttt gtccctgatg
    15901 ggtgaagaaa gcgaggattg taccttatgg gggttgtcga tccccagtaa taaaaaaggc
    15961 cggaaaatcc ggccttttga cgcttttgca gtcttatttt tcctgaagac cgagtttttt
    16021 ctccagatag tggatgttag tgccaccatg ctggaagttc tcgtcattca tgatgcggat
    16081 ctgcagatca acgttggttt tgataccgtc gatgatcagc tcctgcagcg cattcttcat
    16141 gcgggcaatc gccacgtcac ggttttcacc gtagcaaatc agcttaccga tcattgagtc
    16201 atagtacggc ggtacggtgt agcccgcgta gatatgagac tcccaacgca ctccaaaacc
    16261 gccaggtgcg tggaaacggg tgattttgcc tgggcttggc aggaaggtgt tcggatcttc
    16321 ggcgttgata cgacattcca ccgcatggcc gcgaacgtga acttcttctt gcttgatcga
    16381 cagcggttga ccggcagcga tacgcagctg ttctttgatc aggtcaacgc cggtgatcat
    16441 ttctgtaacc gggtgttcta cctgaatacg ggtgttcatt tcgatgaaat agaactcgcc
    16501 gttttcgaac aggaactcga aagtacctgc accgcgatag ccgatatcaa cacacgcttt
    16561 agcacaacgt tcgccgatgt agcgacgcag ttccggggta atgcccggtg ctggtgcttc
    16621 ttcgaccact ttctggtggc ggcgctgcat ggagcagtca cgttccgcca gatagatagc
    16681 gttgccctga ccgtcagcca gtacctgaat ctcgacgtgg cgaggatttt ccaggtattt
    16741 ctccatgtaa accatatcgt tgctgaaagc agctttcgct tccgcacggg tcatggagat
    16801 ggattgtgcc agttcagcgt cgccgcgcac tacgcgcata ccgcgaccac cgccgccgcc
    16861 ggaggctttg ataatcaccg gataaccaat gcgtttagca atggcacggt ttttatccat
    16921 atcgtcgccc agcgggccgt cagaacccgg tacgcaagga acgcccgctt ttttcatcgc
    16981 cgcgattgcg gatactttgt cgcccatcag gcgaatggtt tctgctttcg ggccgatgaa
    17041 gataaagccg gagcgttcaa cctgctcggc aaagttggcg ttctcggaga ggaagccgta
    17101 acccggatgg attgctactg cgccggtgat ttcagcggcg ctgatgattg ccgggatgtt
    17161 cagataactt tttactgacg gagcagggcc gatacagacc gtttcatctg ccagtaatac
    17221 gtgttttaga tcgcgatccg cgctggagtg cacagcgaca gtcttgatgc ccagttcttt
    17281 acaggcacga agaatacgca atgcaatctc gccgcggttg gcaataacaa ttttatccag
    17341 catgttcgcc tcgttactcg atgacgacca gcggctcgtc aaattctacc ggttgtccac
    17401 tttcgaccag aattgctttc acggtaccgg atttgtccgc ttcgatctgg ttcatcattt
    17461 tcatggcttc aacgatgcac agggtatcgc ccacgttgac tttctgaccc acttcgatga
    17521 acgcttttgc gtccgggctt ggggtgcggt agaaagtacc aaccatcggg gaacgtacga
    17581 tgtgaccact gatttccgct gctgctggcg cttccatgga aggaacggtc gccggagcgg
    17641 ctgcgttaga ttgagctggc tgctgcatca ttggtgcagc gtaagcttgt tgcatcacag
    17701 ggaaacttgc ggcaggagct gcacggctaa tgcgtactga ctcttcgcct tcagaaattt
    17761 ccagttcgga gatgcctgat tcttcaacca gctcgatcag ttttttaatc ttacgaatat
    17821 ccatgagtgg gttccgtact ctttgtttag tgtgattgtg acaggcgttt caccgccgtc
    17881 tgtaaagcgt atgccccgac agctgtatgc atagcgataa attccagcag gccgggacgc
    17941 cggtctattt tgccccagag tgtcaatgtt agacttgacg gacattgtgc agcgatctgc
    18001 tgctatcctc cggcaaaaaa caaaatatac cttcgttgcg ccaatgtcac cttttccgca
    18061 gcgcaaaaac tgcgtcaggg aggacgaggt cgcacattat aacgatttcg tagcaattgg
    18121 cagctaaata ctggtcttat cagggaagat aatcaacagc taacatgtaa ataaccttca
    18181 acaccgtgta atttgcaaca agccgcacaa ttcacgaaat tagcgccatc atcgacggaa
    18241 cttcttataa cgtaaggtga aaagctgtaa agccagccct gcgaagatga ggggctgcaa
    18301 tgagataatc ttcacagacc acaaataatg tagggatgcc aggatcgcga caagatagac
    18361 gacattacgc agcggttgct ggggattacc caattttcac tgcattgtct ggatagatgt
    18421 taatgctaaa gcacgcaaaa ttaaccgatt gacaatgctc agcattaaat aggttcgagt
    18481 gattaatttg tagtcaaata aaatcagatt attcacccca tggtaccaga aatcagtaac
    18541 aggttaagcg tagtgtcgcc caggcaaagc accataaacc taacaggcga cggaggcata
    18601 tcaataatgg ctgtttagag gagtatcaat gcgcatcaat ttgtggtcag tcgaaatcat
    18661 gactttccag tttggatgat gttgtacaac ttcctgacca aggtgttcga cgaaagctgt
    18721 ccggctaacc gcttttcccg ttaaccgatt gaataatgat gtcaaaatga gttccctctc
    18781 ttattattcc ttgctaaatg gttagttaac cttcaccagc gtgcgaccct ggatctggtt
    18841 attaatgatg gcctcggcaa agttcggtgc ctctgacaga gatatctctt ttgccgcctg
    18901 ggtatagaat gattccggta aatcggcgac cagtcgctgc caggcttgtg cgcggcgttc
    18961 tggtggcgtc attactgaat ccaccccttg caaacggaca ttacgcagaa taaatggcat
    19021 gaccgtggtt ggcagagtaa aaccacccgc cagaccacag gccgccacgc agccgccgta
    19081 attcatttgc gccagcactt ttgccagcac tttgtcgcca acagtgtcaa ttgccccagc
    19141 ccagacttgt ttttccagag gacgggattc ggcaaactca tcacgaggga gaatacgact
    19201 ggcacctaaa cttttcaggt attcatgggt actttcgcga ccggaaacgg caacgacctg
    19261 ataacccaac ttatgcagca gcgccacggc ggtactgccg acgccaccac tggcacccgt
    19321 cacgacaatc tccccgtcct gcgggcgaac accggcatct tccagcgcca tcacacacag
    19381 catggcggta aaaccggcag taccgataat cattgcttta cgcgcgtcca gcccttgcgg
    19441 catggcaacc agccagtcac ctttcactcg cgcctgctcc gccagcccac cccagtggtt
    19501 ttcaccaacg ccccagccag tgagtaacac ctcctgaccg gcatgaaaac gcggatcttc
    19561 gctggtgcgt acagttccgg caaaatcgat cccaggaatc atcggaaaat tacggatgat
    19621 ttttccctta ccggtaatcg ccagcgcatc tttatagttc aggctcgacc agtgaacatc
    19681 aaccgtgaca tcgccctccg gcaggcgact ttcgtcaagt gtctgtaccg atgcgagagt
    19741 tttgccgtcc tgctgttcta aaagtaacgc ctgcataagt ggtcctcatg tgcatgatgg
    19801 attggaaaat aatttctgaa gactatactc gctaatggaa aagcaaagcc gatgaagcgc
    19861 aataaattgc gagataaatc tgatttgcta gtatgcccgc ttcctcacta tcggagttaa
    19921 cacaaggatg agattaacga cgaaattttc ggcctttgtt acgctgctca ccgggttaac
    19981 aatttttgtg actttgctgg gctgttcgct aagtttctac aacgcaattc agtataagtt
    20041 tagtcatcgc gttcaggctg tggcgacggc gattgatacc caccttgtgt cgaatgactt
    20101 cagcacatta aggccacaaa ttaccgaatt aatgatgtcg gcagatatcg ttcgtgtaga
    20161 cctgctccat ggtgataaac aggtttatac cctggccaga aatggtagtt atcgtccggt
    20221 tggctccagc gatctgtttc gcgaactgag tgttccgttg ataaagcatc cggggatgtc
    20281 gctgcgtctg gtttatcagg atccgatggg caactatttc cattctttaa tgactaccgc
    20341 gccgctcacg ggggcgattg gctttatcat tgttatgctc ttcctggcgg tacgctggtt
    20401 acaacggcaa cttgccgggc aagaattgct ggaaacccgg gctactcgta tcttaaacgg
    20461 tgagcgtggc tctaatgtgt tgggaaccat ctatgaatgg ccgcccagaa ccagcagtgc
    20521 gctagatacg ctgcttcgtg aaattcagaa cgcacgcgaa caacacagcc gtcttgatac
    20581 gctgatccgc tcttatgccg cccaggacgt gaaaaccggc ctcaataacc gactcttttt
    20641 cgataatcag ttagcaacgt tactggaaga tcaggagaaa gtaggtaccc acgggatcgt
    20701 gatgatgatt cgtctgccgg atttcaatat gttgagcgat acctgggggc acagccaggt
    20761 tgaagaacag ttcttcactc tgacgaatct gctgtcgaca tttatgatgc gctaccctgg
    20821 cgcactgctg gcgcgttacc accgcagtga ttttgctgcg ctgttaccgc accggacgtt
    20881 aaaagaggca gagagcatcg ccggtcagtt aatcaaagcc gtcgatacct tgccgaacaa
    20941 taaaatgctc gatcgcgacg atatgatcca cattggtatc tgcgcctggc gtagtggtca
    21001 ggataccgag caggtaatgg aacatgcaga gtctgccacg cgtaatgcgg gattgcaggg
    21061 cggcaatagc tgggctattt acgatgactc gttgcctgaa aaaggacgcg gtaatgttcg
    21121 ctggcgtacg cttatcgagc aaatgctcag tcgcggcggc ccgcgccttt atcaaaaacc
    21181 ggcggttact cgcgaaggtc aggttcatca tcgcgaactc atgtgccgca tcttcgatgg
    21241 taatgaagag gttagctcgg cggagtatat gccgatggtc ttgcagtttg gcttatcgga
    21301 agagtatgac cgtctgcaaa tcagccgtct tattccacta ttgcgttact ggccagagga
    21361 aaatctggcg attcaggtta ccgttgagtc gctgattcgc ccgcgttttc agcgttggct
    21421 gcgcgatacg ttaatgcaat gtgaaaaatc acaacgaaaa cgcataatta ttgaacttgc
    21481 agaggccgat gtaggtcaac atatcagtcg tttacaacct gttattcgtt tagtgaatgc
    21541 tttaggggta cgggtagccg tcaaccaggc tggtttgacg ctggtaagca ccagttggat
    21601 caaagaactt aatgttgagt tactcaagct ccatccgggg ctggtcagaa acattgagaa
    21661 gcgaacggag aaccagctgc tggttcaaag cctggtggaa gcctgctccg ggaccagcac
    21721 ccaagtttac gccaccggtg tgcgttcgcg aagcgagtgg cagaccctga ttcagcgcgg
    21781 tgttacaggc gggcaagggg attttttcgc gtcctcacag ccacttgata ctaacgtgaa
    21841 aaaatattca caaagatact cggtttaacc tgccgtttaa tctgttttca cgtagaataa
    21901 cgcgcgctgc gtctcatggg agtgtgcttg tctgctcgcc agattgttgc agcacatatg
    21961 cagatgaatg accttacgcg gttgcaaaca ggcgaggaat gctgctgatg cattaagcct
    22021 ttctggactc aggcagagat ttgtaacaaa ggaaacgaac tgcactaatt ttcaccgtag
    22081 cagatgattt ttgcgccttg tcgctgctgc gtgtggttgg taaagtaagc ggattttctt
    22141 ttccgcccca gctttcagga ttatccctta gtatgttgaa aaaatttcgt ggcatgtttt
    22201 ccaatgactt gtccattgac ctgggtactg cgaataccct catttatgta aaaggacaag
    22261 gcatcgtatt gaatgagcct tccgtggtgg ccattcgtca ggatcgtgcc ggttcaccga
    22321 aaagcgtagc tgcagtaggt catgacgcga agcagatgct gggccgtacg ccgggcaata
    22381 ttgctgccat tcgcccaatg aaagacggcg ttatcgccga cttcttcgtg actgaaaaaa
    22441 tgctccagca cttcatcaaa caagtgcaca gcaacagctt tatgcgtcca agcccgcgcg
    22501 ttctggtttg tgtaccggtt ggcgcgaccc aggttgaacg ccgcgcaatt cgtgaatccg
    22561 cgcagggcgc tggtgcccgt gaagtcttcc tgattgaaga accgatggct gccgcaattg
    22621 gtgctggcct gccggtttct gaagcgaccg gttctatggt ggttgatatc ggtggtggta
    22681 ccactgaagt tgctgttatc tccttgaacg gcgtggttta ctcctcttcc gtgcgcattg
    22741 gtggtgatcg tttcgacgaa gctatcatca actatgtgcg tcgtaattac ggttctctga
    22801 tcggtgaagc caccgcagaa cgtatcaagc acgaaatcgg ttcggcttac ccgggcgatg
    22861 aagtccgtga aatcgaagtt cgtggccgta acctggcaga aggtgttcca cgcggtttta
    22921 ccctgaactc caacgaaatc ctcgaagcac tgcaggaacc gctgaccggt attgtgagcg
    22981 cggtaatggt tgcactggaa cagtgcccgc cggaactggc ttccgacatc tccgagcgcg
    23041 gcatggtgct caccggtggt ggcgcactgc tgcgtaacct tgaccgtttg ttaatggaag
    23101 aaaccggcat tccagtcgtt gttgctgaag acccgctgac ctgtgtggcg cgcggtggcg
    23161 gcaaagcgct ggaaatgatc gacatgcacg gcggcgacct gttcagcgaa gagtaatcgg
    23221 atgcaggcag gggaaatgtc tgtttaccct gcctggtctg atacgagaat acgcataact
    23281 tatgaagcca atttttagcc gtggcccgtc gctacagatt cgccttattc tggcggtgct
    23341 ggtggcgctc ggcattatta ttgccgacag ccgcctgggg acgttcagtc aaatccgtac
    23401 ttatatggat accgccgtca gtcctttcta ctttgtttcc aatgctcctc gtgaattgct
    23461 ggatggcgta tcgcagacgc tggcctcgcg tgaccaacta gaacttgaaa accgggcgtt
    23521 acgtcaggaa ctgttgctga aaaacagtga actgctgatg cttggacaat acaaacagga
    23581 gaacgcgcgt ctgcgcgagc tgctgggttc cccgctgcgt caggatgagc agaaaatggt
    23641 gactcaggtt atctccacgg ttaacgatcc ttatagcgat caagttgtta tcgataaagg
    23701 tagcgttaat ggcgtttatg aaggccagcc ggtcatcagc gacaaaggtg ttgttggtca
    23761 ggtggtggcc gtcgctaaac tgaccagtcg cgtgctgctg atttgtgatg cgacccacgc
    23821 gctgccaatc caggtgctgc gcaacgatat ccgcgtaatt gcagccggta acggttgtac
    23881 ggatgatttg cagcttgagc atctgccagc gaatacggat attcgtgttg gtgatgtgct
    23941 ggtgacctcc ggtctgggcg gtcgtttccc ggaaggctat ccggtcgcgg ttgtctcttc
    24001 cgtaaaactc gatacccagc gcgcttatac tgtgattcag gcgcgtccga ctgcaggact
    24061 gcaacgtttg cgttatctgc tgctgctgtg gggcgcggat cgtaacggcg ctaacccgat
    24121 gacgccggaa gaggtgcatc gtgttgctaa tgaacgtctg atgcagatga tgccgcaggt
    24181 attgccttcg ccagacgcga tggggccaaa gttacctgaa ccggcaacgg ggatcgctca
    24241 gccgactccg cagcaaccga cgacaggaaa tgcagctact gcgcctgctg cgcccacaca
    24301 gcctgctgct aatcgctctc cacaaagggc tacgccgccg caaagtggtg ctcaaccgcc
    24361 tgcgcgtgcg ccgggagggc aatagtggcg agctatcgta gccagggacg ttgggtaatc
    24421 tggctctctt tcctcattgc gctgttgctg caaatcatgc cctggccgga taacctgatt
    24481 gttttccggc caaactgggt gttactcatc ttgttgtatt ggatcctggc cttgcctcat
    24541 cgcgtaaatg tgggcacagg ttttgtgatg ggtgccatac tggatctgat cagcggctcg
    24601 acgcttggcg tacgcgtatt ggcgatgagc atcattgctt acctggtggc gctgaaatac
    24661 cagcttttcc gcaacctcgc attatggcag caggcgctgg tcgtcatgtt gctttcgctg
    24721 gtggtggata ttattgtttt ctgggcagag tttttagtga ttaacgtctc tttcagaccg
    24781 gaagtgttct ggagtagtgt agtcaatggg gtgctctggc cgtggatttt cttgctgatg
    24841 cgtaaagtcc gtcagcagtt tgcagtgcaa taaaggtttc tatgacttct ctgtatttag
    24901 cttccggttc tccgcgtcgt caggagttac ttgcgcaact tggcgtgacc tttgaacgta
    24961 ttgttacagg cattgaggag cagcgtcagc cgcaggagag cgcgcagcag tatgttgtgc
    25021 gtctggcgcg cgagaaagca caggcgggtg tcgcgcaaac ggcgcaggat ctcccggtgc
    25081 tgggtgcgga tactatcgtt atcctgaatg gagaagtgct ggagaaaccg cgcgacgcag
    25141 agcatgcggc gcagatgttg cgcaaattat cgggtcagac ccatcaggtg atgacggcag
    25201 tggcgttggc cgacagccag cacattctcg attgcctggt ggtcaccgat gtgactttca
    25261 gaacgttaac agacgaagac atcgcgggct atgtcgccag cggtgaaccg ttagataaag
    25321 caggtgcata cggtattcag gggctgggtg gctgttttgt caggaagata aatggcagct
    25381 atcacgccgt agtcggctta ccgctggttg aaacgtatga attattaagt aattttaacg
    25441 cactgcgtga gaaaagggat aaacatgacg gctgaattgt tagtaaacgt aacgccttcg
    25501 gaaacgcgag tggcgtatat tgatggcggt attctgcagg aaattcatat tgaacgtgag
    25561 gcgcgacgcg gaatagtagg caatatctac aagggtcgtg taagtcgtgt acttccgggt
    25621 atgcaggcgg cttttgtaga tattgggctg gataaagccg cgtttcttca tgcatccgat
    25681 atcatgccgc acaccgaatg tgtggcgggt gaagaacaaa agcaattcac ggtgcgcgac
    25741 atctcggaac tggttcgtca ggggcaagat ctgatggtgc aggtggtgaa agatccgctt
    25801 ggcactaaag gtgcgcgcct gaccaccgat atcacgctgc cttctcgcta tctggtgttt
    25861 atgccagggg cttctcacgt tggggtttcc caacgtattg aaagcgaatc agaacgtgaa
    25921 cgcctgaaaa aagtggtcgc agagtattgc gacgagcagg gcgggtttat catccgtacc
    25981 gcagcggaag gggttggcga ggctgaactg gcctccgatg ccgcttatct gaaacgcgtc
    26041 tggaccaaag ttatggagcg taaaaaacgc ccgcagaccc gttatcagct gtacggcgaa
    26101 ctggcgctgg cgcagcgtgt tctgcgtgat ttcgccgacg ccgaactgga ccgcattcgc
    26161 gttgactcac gcctgactta cgaagcattg ctggagttta cctcggagta cattcccgag
    26221 atgaccagca agctggagca ttacaccgga cgccagccga ttttcgatct ctttgatgtc
    26281 gaaaacgaaa tccagcgagc gctggaacgc aaagtagaac tgaaatccgg tggttatctg
    26341 attatcgacc agaccgaagc gatgaccacc gtggacatca ataccggagc gtttgtcggt
    26401 catcgcaatc tggacgacac cattttcaat accaatattg aagcgacgca ggctatcgct
    26461 cgccagttac ggttgcgtaa tctgggtggg attatcatta ttgatttcat cgatatgaat
    26521 aatgaagatc accgccgccg cgtgctgcac tcgctggagc aggcgttgag caaagaccgg
    26581 gtgaaaacca gcgttaatgg tttttcggcg ctggggctgg tggagatgac gcgtaaacgc
    26641 acccgcgaaa gcattgagca cgtactgtgt aacgaatgcc caacctgcca cggtcgcgga
    26701 acggtgaaaa ccgtggaaac ggtatgctat gaaatcatgc gcgagattgt tcgtgtccac
    26761 catgcttacg actccgaccg tttcctggtc tatgcttctc cggcagtagc tgaagccttg
    26821 aaaggcgaag agtcacactc gctggcggaa gtggaaattt tcgttggcaa acaggttaaa
    26881 gtacaaattg aaccgctcta taaccaggag cagtttgacg tcgtaatgat gtaaacagat
    26941 gctgggccgc catccggcaa agggtttttg agtcacattt ttagcagaca aggagtgacg
    27001 ggtgaggcga ttgccgggga ttttactgct tactggagcc gcgctcgttg tgatcgctgc
    27061 tctgctggtt agcggcctgc gtattgcttt accgcatctt gacgcctggc gtccggaaat
    27121 cctcaacaaa atagaatccg cgactggcat gccggtagaa gccagtcagc tctcagccag
    27181 ctggcagaat tttggcccga cgcttgaagc acacgacatc cgtgcagaac taaaagatgg
    27241 cggcgaattt tcggttaaac gcgttactct ggcgctggat gtctggcaga gcctgttaca
    27301 tatgcgctgg cagtttcgcg acctcacttt ctggcagctg cgctttcgca ccaacactcc
    27361 tatcaccagc ggtggtagcg atgacagtct ggaagccagt cacatcagcg atctgtttct
    27421 tcgtcaattt gaccatttcg atctccgcga cagtgaagtc agttttctga cgccatccgg
    27481 tcagcgcgcc gagctggcga tcccacaact cacctggctg aacgatccac gtcgacaccg
    27541 tgcggaaggc ctggtaagcc tctccagcct taccggacag cacggcgtga tgcaggtgcg
    27601 tatggatttg cgcgatgatg aggggttgtt aagcaatggt cgcgtctggc tacaggcgga
    27661 tgacatcgac ctgaagccgt ggctcggtaa atggatgcag gacaatattg ctctggaaac
    27721 ggcacagttc tcccttgaag gctggatgac gatcgacaaa ggcgatgtaa ccggcggtga
    27781 cgtctggctg aaacagggcg gtgccagctg gttgggcgag aagcaaacgc atacgctgtc
    27841 ggtggataat ctgaccgcgc atattacgcg tgaaaatccg ggctggcagt tctctattcc
    27901 cgatacacgg atcacgatgg acggcaaacc ctggccgagc ggagcattga cgctggcctg
    27961 gataccggaa caggacgttg gcggcaaaga caataaacgc agtgacgaac tccggattcg
    28021 cgccagtaat ctggagctgg caggcctgga gggcatacgc ccgctggccg cgaaactttc
    28081 acctgcactg ggtgatgttt ggcgctccac acaaccgagc ggcaagatta acactctggc
    28141 gctggatatc ccgcttcagg cggcagacaa tacccgtttt caggcatcgt ggagcgatct
    28201 ggcctggaag caatggaaat tattaccggg tgcggaacac ttctccggga cgctttccgg
    28261 cagcgttgaa aatggtttgc ttaccgcgtc gatgaagcag gcaaagatgc cttacgaaac
    28321 ggtattccgt gcgccactgg aaatcgccga cggccaggca actataagct ggctgaacaa
    28381 tgacaaaggt ttccagctgg atgggcgtaa tattgacgtt aaagccaaag ccgtccatgc
    28441 gcgcggcggt tttcgttacc tgcaacctgc taacgatgaa ccctggctgg gtattctggc
    28501 tggcatcagt accgatgatg gttcacaagc ctggcgctat ttcccggaaa acttgatggg
    28561 taaagacctg gttgattact taagtggcgc gattcagggc ggtgaagcgg ataacgcgac
    28621 gctggtttat ggtggcaatc cgcaactctt cccctataaa cacaacgaag gtcagtttga
    28681 agtgctggtg ccgctgcgca acgcgaagtt tgccttccag ccggactggc ctgcattaac
    28741 taaccttgat attgaactgg actttattaa cgacggttta tggatgaaaa ccgatggcgt
    28801 taatctgggc ggcgtgcgcg cgagtaatct caccgcagtg atccctgact actcaaaaga
    28861 aaaactgctg attgacgctg acattaaagg tccgggtaaa gccgtaggcc cttactttga
    28921 tgagacaccg ctgaaagatt ctctgggtgc gaccctgcaa gaactccagc tcgacggcga
    28981 tgtgaatgct cgcttacatc ttgatatccc gctgaacggc gagctggtaa ccgcgaaagg
    29041 tgaagtgacg ctgcgtaata acagtctgtt tatcaaacta ctcgacagca ccctgaaaaa
    29101 tttgagcggt aaattcagct ttatcaatgg cgatctgcaa agtgaaccac tgacagcaag
    29161 ctggtttaat cagccgttga acgtggattt ttccaccaaa gaaggggcaa aagcctacca
    29221 ggttgcggtg aatctcaacg gtaactggca accggcgaaa accggcgttc tgcctgaagc
    29281 ggtgaacgaa gcattgagtg gcagcgtggc gtgggatggt aaagtgggca ttgagctgcc
    29341 ttatcatgct ggcgcgacct ataacgtaga gctgaacggc gatctgaaga atgtgagcag
    29401 tcacttacct tcaccgttag ccaaacctgc gggtgaacca ctagcggtaa acgttaaggt
    29461 tgatggcaat ctcaacagct ttgaattaac cggacaggct ggtgcggata accatttcaa
    29521 tagccgctgg ttgcttggtc aaaagctgac gctcgatcgt gctatttggg cggcagacag
    29581 taaaacgctc ccgccgttgc cggaacaaag tggcgttgaa ctcaatatgc cgccgatgaa
    29641 tggtgccgag tggctggccc tgttccagaa aggcgctgcg gagagtgtcg gtggtgcagc
    29701 gagtttccca caacacataa cgttacgtac gcctatgttg tcactgggaa atcagcaatg
    29761 gaataacctg agtattgttt cgcaaccgac ggcaaatggc accctggttg aggcgcaggg
    29821 gcgtgaaatc aatgccacgc tggcgatgcg taataacgcg ccgtggctgg cgaatatcaa
    29881 atatctttat tacaacccga gcatggcgaa aactcgtggt gattcaacac cgtcatcacc
    29941 tttcccgaca acggagcgca ttaacttccg tggctggccg gacgcacaaa tacgatgcac
    30001 agagtgctgg ttctgggggc aaaaattcgg tcgtattgac agtgatctca ccatttctgg
    30061 cgatacgtta acgctgacca atggactgat tgatactggt ttctcgcggc ttactgccga
    30121 tggtgaatgg gttaataatc cggggaatga acgtacctcg ctgaaaggaa aactgcgcgg
    30181 gcagaaaatt gatgccgccg cagaattttt tggtgtcacg acgcccatac gccagtcgtc
    30241 atttaatgtg gattacgatt tacactggcg caaagcaccc tggcaaccag atgaggcgac
    30301 gttgaatggc atcattcata ctcaactggg taaaggcgaa attaccgaaa tcaataccgg
    30361 acatgccggg caattgctgc gcttattgag cgttgatgcc ctgatgcgta agctgcgttt
    30421 tgatttcaga gacacttttg gcgaagggtt ctattttgac tccattcgca gcaccgcgtg
    30481 gattaaagac ggggttatgc acaccgacga cacgctggtg gatggcctgg aggcggatat
    30541 cgccatgaaa gggtcggtaa atctggtacg tcgcgacctg aatatggaag cggttgtcgc
    30601 accagagatt tctgcgacgg tgggcgtggc tgcggctttt gcggttaacc ccattgttgg
    30661 cgcggcagta tttgcagcca gtaaagtgct ggggccgctg tggagcaaag tctccatttt
    30721 gcgctatcac atttcgggtc cgctggacga tccgcaaatc aacgaagtgt tgcgccaacc
    30781 gcgtaaagaa aaagcgcaat gatttgacga gggcgcgtaa ttgccccaat ctcatatgat
    30841 aatcgttgcc aaaggccaac gagccggaac ataactgtag gccggatagg gcgttcacgc
    30901 cgcatccggc agccgtaaaa aatcctctac tgcagtaact aacgagtagc aaaaacgatg
    30961 agtctgaacc tggtaagtga acaattgcta gcggcgaacg gcctgaaaca tcaggacttg
    31021 ttcgcgatcc tcggtcaact ggccgaacgt cgccttgatt atggcgatct ctattttcag
    31081 tcgagctatc acgaatcctg ggttttagaa gaccgcatta ttaaagatgg ttcttacaac
    31141 atcgatcagg gcgttggtgt gcgtgcaatc agtggtgaaa aaaccggatt tgcttacgct
    31201 gaccaaatca gcctgctggc gctggaacag agtgcgcaag cggcgcgcac catcgtccgt
    31261 gatagtggtg atggcaaagt acagacgctg ggcgcggtag agcatagccc gttgtatacc
    31321 tcggtagatc cgctgcaaag catgagccgt gaagagaagc tggatatcct gcgtcgcgtc
    31381 gataaggttg cccgcgaagc ggacaagcgc gtacaagaag tgactgccag cctcagcggc
    31441 gtttatgaat taattctggt tgcggccacc gacggcacgc tggcggcgga tgttcgtccg
    31501 ctggtgcgtc tttccgtgag cgttctggtc gaagaagatg gcaaacgcga acgcggtgcc
    31561 agtggcggcg gcggtcgttt tggttatgag ttcttccttg ccgatcttga cggcgaagtc
    31621 cgtgcggatg catgggcaaa agaagcagtg cgtatggcgc tggtcaatct ttctgccgtt
    31681 gctgcaccag cgggcaccat gccggtagta cttggcgcag gttggccggg cgtgctgttg
    31741 catgaagcgg taggtcacgg tctggaaggc gacttcaacc gccgcggcac ttcagtattt
    31801 agtgggcagg tcggggagct ggtggcttca gaactgtgta ccgtggttga tgacggcacg
    31861 atggtcgatc gccgtggttc ggtggcgatt gatgacgaag gtacgccagg ccagtacaac
    31921 gtgctgattg agaacggcat tctgaaaggc tacatgcagg ataaactcaa cgcgcgtttg
    31981 atggggatga cgccgactgg caacggtcgc cgtgaatcct acgcccatct gcccatgccg
    32041 cgtatgacca acacctatat gctgccgggt aaatcgaccc cgcaggaaat tattgaatcc
    32101 gttgagtacg gtatctatgc accgaacttt ggtggcggtc aggtggatat tacatccggc
    32161 aaattcgttt tctccacttc agaagcgtat ctgattgaaa acggtaaagt aacgaagccg
    32221 gtgaaaggcg cgacgttgat tggttccggt atcgaaacca tgcagcagat ttcgatggtt
    32281 ggcaacgacc tgaaactgga taacggcgtg ggtgtctgcg gtaaagaagg gcaaagtttg
    32341 ccggttggcg tgggccagcc aacgctgaaa gttgataacc tgactgttgg cggtactgcg
    32401 taataatttc attattttca gaaggataat taatctttct acgtgcacga acggtcccct
    32461 cgctcctctg gggttagggt gaggggaacc cgttggcaca ggtttgtaga acgtaacagt
    32521 acaatatgaa ttacttctct ttcccgcgcc cgtgcatctc ctgaaacaat ttaccgacct
    32581 caacaaaata atccgtcagc gagttgatca cgacctgtac cttcagcggc agcttatctt
    32641 tttcggtata taacgcataa accgggcgtg gatctgactg ataacgcggc agcaggatct
    32701 ccagctcacc gcgattgatc tcgttgatca cccacatcag cggcacgtag gcgatcccgg
    32761 cacccgccgt tagccagcgc accagcgtca tcggatcatt agtcacaaat ctcccttgcg
    32821 ggatcaggcg agtcgagatc ccttccggtg caatcagttc aaattcattg tcgggccgca
    32881 cgctgtattc aagccatgaa tggctactta aatcggcggg tttttccggt atgccgtatt
    32941 gtgtgagata gcttttcgcg gcgcacacca ccattggcat cgcgccaaga cggcgggaaa
    33001 acaggctgga atcctgcaac gcgccgacgc ggatcaccac atccagaccg tcggcaatca
    33061 ggtcgggggc tggaattccg gtaaccagat tgacgctcaa acctgggtat tctttcagca
    33121 ttttggctgt cagcccggcg agaacatttt gtgccatagt tgaagaacag ccaatgcgta
    33181 gcgtcccgat gggggtgtta ttgaaggcat acagttgctc atgaacatcc tgcacttcat
    33241 gaagcatacg acggcagccc tggtagtaaa ttctaccggc ttcggtcagg ccaatgctgc
    33301 gtgtgctacg gtttaacagc tttacctgca actcatcttc cagttttgac accgtctgac
    33361 tgatggacga aacgctcatc tgtagctgtc tggcggcggc ggtaaaagag ccaaattcaa
    33421 ctactttggc aaacaccgac atgcgtttta gtcgttccat tattcactct gacttaaaag
    33481 tgatttagat cacataatat agataacagc ataacagtta cgctaatata ttaaatatca
    33541 atctacagca atgttgctct tgcccggctt tccatgccct tctctgcggc gacagatgct
    33601 gaaaataata acgcctgctc tctctataca accaaggtca acatgagtct gtttcccgtt
    33661 atcgtggtgt ttgggctgtc cttcccaccg atattttttg aattgctttt atcactggcg
    33721 attttctggc tggtgcgccg ggtacttgtg ccaacaggta tctacgactt tgtctggcat
    33781 ccggcgttgt tcaacaccgc gctctattgc tgcttgtttt atttgatatc gcgactgttc
    33841 gtttgaggtt gaagtgaaaa cactaataag aaaattctcc cgtacggcca tcacggtcgt
    33901 attagtcatt ctggccttca tcgcaatttt taatgcctgg gtctattaca ccgaatcccc
    33961 ctggacgcgt gacgcgcgct ttagcgctga cgtcgttgcg atcgcgccgg acgtttctgg
    34021 actcattacc caggtgaatg ttcatgataa ccagctggtg aaaaaaggac agatactgtt
    34081 caccatcgac cagccgcgct atcaaaaggc gcttgaggaa gcgcaagccg atgttgctta
    34141 ttatcaggta ctggcacagg agaaacgcca ggaggccgga cgtcgtaacc gtctcggtgt
    34201 gcaggcgatg tctcgcgaag agatcgacca ggctaacaac gtactacaaa cggttctgca
    34261 tcagttagcg aaagcgcagg cgacccgcga tctggcaaaa ctggatcttg aacgcacggt
    34321 gatccgcgcg ccagcagatg gctgggtgac caacctcaac gtctataccg gtgagtttat
    34381 tactcgagga tcaacggcgg ttgcgctggt gaaacagaac tccttctatg tactggccta
    34441 tatggaagaa actaagctgg aaggggtgcg tccggggtat cgtgcagaga tcacgccgct
    34501 tggcagtaac aaagtgctga aagggactgt tgatagtgtt gccgctgggg tcaccaacgc
    34561 cagcagcacg cgtgacgaca aagggatggc gaccatcgac tctaaccttg aatgggtgcg
    34621 tttggcgcaa cgtgttccgg ttcgtattcg tctcgacaac cagcaagaga acatctggcc
    34681 tgcgggcacc actgctacag tggtggtcac tggcaaacaa gatcgcgacg aaagccagga
    34741 ttcgttcttc cgtaaaatgg cccatcgcct gcgtgagttt ggttaatcac gatgggtatt
    34801 ttctccattg ctaaccaaca tattcgcttt gcggtaaaac tggcgaccgc cattgtactg
    34861 gcgctgtttg ttggctttca cttccagctg gaaacgccac gctgggcggt actgacagcg
    34921 gcgattgttg ccgccggtcc ggcctttgct gcgggaggtg aaccgtattc tggcgctatt
    34981 cgctatcgtg gctttttgcg catcatcggc acatttattg gctgtattgc cggactggtg
    35041 atcatcattg cgatgatccg cgcaccatta ttgatgattc tggtgtgctg tatctgggcc
    35101 ggtttttgta cctggatatc ctcgctggta cgaatagaaa actcgtacgc gtgggggctg
    35161 gccggttata ccgcgctgat cattgtgatc accattcagc cggaaccatt gcttacgccg
    35221 cagtttgccg tcgaacgttg tagcgagatc gttatcggta ttgtgtgtgc gattatggcg
    35281 gatttgctct tttctccgcg atcgatcaaa caagaagtgg atcgagagct ggaaagtttg
    35341 ctggtcgcgc aatatcaatt aatgcaactc tgtatcaagc atggcgatgg tgaagttgtc
    35401 gataaagcct ggggcgacct ggtgcgacgc accacggcgc tacaaggcat gcgcagcaac
    35461 ctgaatatgg aatcttcccg ctgggcgcgg gccaatcgac gtttaaaagc gatcaatacg
    35521 ctatcgctga cgctgattac ccaatcctgc gaaacttatc ttattcagaa tacgcgcccg
    35581 gaattgatca ctgatacttt ccgcgaattt tttgacacgc cggtagaaac cgcgcaggac
    35641 gtccacaagc agctcaaacg cctgcggaga gttatcgcct ggaccgggga acgggaaacg
    35701 cctgtcacca tttatagctg ggtcgcggcg gcaacgcgtt atcagcttct caagcgcggc
    35761 gttatcagta acacaaaaat caacgccacc gaagaagaga tcctgcaagg cgaaccggaa
    35821 gtcaaagtag agtcagccga acgtcatcat gcgatggtta acttctggcg aaccacactt
    35881 tcctgcattc tgggcacgct tttctggctg tggacgggct ggacttccgg cagtggtgca
    35941 atggtgatga ttgcggtagt gacgtcactg gcaatgcgtt tgccgaatcc acgcatggtg
    36001 gcgatcgact ttatctacgg gacgctggcc gcgctgccgt tagggctgct ctactttttg
    36061 gtgattatcc ctaataccca acagagcatg ttgctgctgt gtattagcct ggcagtgctg
    36121 ggatttttcc tcggtataga agtacagaaa cggcgactgg gctcgatggg ggcgctggcc
    36181 agcaccataa atattatcgt gctggataac ccgatgactt tccatttcag tcagtttctc
    36241 gacagcgcat tagggcaaat cgtcggctgt gtgctcgcgt tcaccgttat tttgcttgtg
    36301 cgggataaat cgcgcgacag gaccggacgt gtactgctta atcagtttgt ttctgccgct
    36361 gtttccgcga tgactaccaa tgtggcacgt cgtaaagaga accacctccc ggcactttat
    36421 cagcagctgt ttttgctgat gaataagttc ccaggggatt tgccgaaatt tcgcctggcg
    36481 ctgacgatga ttatcgcgca ccagcgcctg cgtgatgcgc cgatcccggt taacgaggat
    36541 ttatcggcgt ttcaccgaca aatgcgccgc acagcagacc atgtgatatc tgcccgtagc
    36601 gatgataaac gtcgtcggta ctttggccag ttgctggaag aactggaaat ctaccaggaa
    36661 aagctacgca tctggcaagc gccaccgcag gtgacggaac cggttcatcg gctggcgggg
    36721 atgctccata agtatcaaca tgcgttgacc gatagttaag tcaaaaccga cgccaaaagc
    36781 gtcggttttt tcatggctat acttagcgat gcacggcaga actcgccgcg aaacgtgacg
    36841 gtggcaacag atgaatattt atacctttga ttttgatgag attgagagtc aggaggattt
    36901 ttatcgcgac ttcagccaag cctttggtct ggcgaaagat aaggtacgcg atctcgactc
    36961 actatgggat gtgttaatga acgatgtcct gccgctacca cttgagattg aatttgttca
    37021 tctgggagag aaaacgcgtc gccgttttgg cgcgttaata ttgctgtttg atgaggcaga
    37081 ggaagagctg gaagggcatt tgcgttttaa tgttcgtcat tagcgcaaaa aaaagccccc
    37141 gaaccggggg caatatcgtc ggacaagacg atgagggttt atttgtacag ttcagccgta
    37201 gcgtgccagg tgtcaccgct acgagcttca gtaatctggt aggccgttgc gcctttctct
    37261 tccgcttttt tgttcagcat ttcacgcata tccattggcg aagacgccac accacttacg
    37321 gatacggtcc cgattgcttc acgattttgt gcttgtgcag catcaatgga gtcggcagcg
    37381 aatgcaccga aagagagaac agaaagtacg cttaatgcag ctacagtggt tttgattttc
    37441 atgattttta cctcgacata atcttttagc tgggcctttg tttcgtgacc catatcacaa
    37501 aatcaagtat acactaatta ttgaactaat taata
//
LOCUS       DAZGXZ010000033        37450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000033 DAZGXZ010000000
VERSION     DAZGXZ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.033"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            217..1590
                     /gene="aroP"
                     /locus_tag="ACHKJF_001803"
     CDS             217..1590
                     /gene="aroP"
                     /locus_tag="ACHKJF_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP0949174.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1633..2487)
                     /gene="ampE"
                     /locus_tag="ACHKJF_001804"
     CDS             complement(1633..2487)
                     /gene="ampE"
                     /locus_tag="ACHKJF_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP0949175.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2484..3035)
                     /gene="ampD"
                     /locus_tag="ACHKJF_001805"
     CDS             complement(2484..3035)
                     /gene="ampD"
                     /locus_tag="ACHKJF_001805"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP0949176.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3123..4016
                     /gene="nadC"
                     /locus_tag="ACHKJF_001806"
     CDS             3123..4016
                     /gene="nadC"
                     /locus_tag="ACHKJF_001806"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP0949177.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4219..4659
                     /gene="ppdD"
                     /locus_tag="ACHKJF_001807"
     CDS             4219..4659
                     /gene="ppdD"
                     /locus_tag="ACHKJF_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP0949178.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4669..6054
                     /gene="gspE"
                     /locus_tag="ACHKJF_001808"
     CDS             4669..6054
                     /gene="gspE"
                     /locus_tag="ACHKJF_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP0949179.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6044..7246
                     /gene="hofC"
                     /locus_tag="ACHKJF_001809"
     CDS             6044..7246
                     /gene="hofC"
                     /locus_tag="ACHKJF_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP0949180.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPCWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7281..8324)
                     /gene="guaC"
                     /locus_tag="ACHKJF_001810"
     CDS             complement(7281..8324)
                     /gene="guaC"
                     /locus_tag="ACHKJF_001810"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP0949181.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8480..8524)
                     /locus_tag="ACHKJF_001811"
     CDS             complement(8480..8524)
                     /locus_tag="ACHKJF_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949182.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8549..9169
                     /gene="coaE"
                     /locus_tag="ACHKJF_001812"
     CDS             8549..9169
                     /gene="coaE"
                     /locus_tag="ACHKJF_001812"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP0949183.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9169..9912
                     /gene="zapD"
                     /locus_tag="ACHKJF_001813"
     CDS             9169..9912
                     /gene="zapD"
                     /locus_tag="ACHKJF_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP0949184.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9922..10119
                     /gene="yacG"
                     /locus_tag="ACHKJF_001814"
     CDS             9922..10119
                     /gene="yacG"
                     /locus_tag="ACHKJF_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP0949185.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10219..10608)
                     /gene="mutT"
                     /locus_tag="ACHKJF_001815"
     CDS             complement(10219..10608)
                     /gene="mutT"
                     /locus_tag="ACHKJF_001815"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP0949186.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10668..13373)
                     /gene="secA"
                     /locus_tag="ACHKJF_001816"
     CDS             complement(10668..13373)
                     /gene="secA"
                     /locus_tag="ACHKJF_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP0949187.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13435..13947)
                     /gene="secM"
                     /locus_tag="ACHKJF_001817"
     CDS             complement(13435..13947)
                     /gene="secM"
                     /locus_tag="ACHKJF_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP0949188.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14178..15095)
                     /gene="lpxC"
                     /locus_tag="ACHKJF_001818"
     CDS             complement(14178..15095)
                     /gene="lpxC"
                     /locus_tag="ACHKJF_001818"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP0949189.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15196..16347)
                     /gene="ftsZ"
                     /locus_tag="ACHKJF_001819"
     CDS             complement(15196..16347)
                     /gene="ftsZ"
                     /locus_tag="ACHKJF_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP0949190.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16408..17670)
                     /gene="ftsA"
                     /locus_tag="ACHKJF_001820"
     CDS             complement(16408..17670)
                     /gene="ftsA"
                     /locus_tag="ACHKJF_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP0949191.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17667..18497)
                     /gene="ftsQ"
                     /locus_tag="ACHKJF_001821"
     CDS             complement(17667..18497)
                     /gene="ftsQ"
                     /locus_tag="ACHKJF_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP0949192.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYAPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18499..19419)
                     /gene="ddlB"
                     /locus_tag="ACHKJF_001822"
     CDS             complement(18499..19419)
                     /gene="ddlB"
                     /locus_tag="ACHKJF_001822"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0949193.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19412..20887)
                     /gene="murC"
                     /locus_tag="ACHKJF_001823"
     CDS             complement(19412..20887)
                     /gene="murC"
                     /locus_tag="ACHKJF_001823"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP0949194.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(20941..22008)
                     /gene="murG"
                     /locus_tag="ACHKJF_001824"
     CDS             complement(20941..22008)
                     /gene="murG"
                     /locus_tag="ACHKJF_001824"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP0949195.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22005..23249)
                     /gene="ftsW"
                     /locus_tag="ACHKJF_001825"
     CDS             complement(22005..23249)
                     /gene="ftsW"
                     /locus_tag="ACHKJF_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP0949196.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23249..24565)
                     /gene="murD"
                     /locus_tag="ACHKJF_001826"
     CDS             complement(23249..24565)
                     /gene="murD"
                     /locus_tag="ACHKJF_001826"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP0949197.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24568..25650)
                     /gene="mraY"
                     /locus_tag="ACHKJF_001827"
     CDS             complement(24568..25650)
                     /gene="mraY"
                     /locus_tag="ACHKJF_001827"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP0949198.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25644..27002)
                     /gene="murF"
                     /locus_tag="ACHKJF_001828"
     CDS             complement(25644..27002)
                     /gene="murF"
                     /locus_tag="ACHKJF_001828"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP0949199.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYNYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(26999..28486)
                     /gene="murE"
                     /locus_tag="ACHKJF_001829"
     CDS             complement(26999..28486)
                     /gene="murE"
                     /locus_tag="ACHKJF_001829"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP0949200.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28473..30239)
                     /gene="ftsI"
                     /locus_tag="ACHKJF_001830"
     CDS             complement(28473..30239)
                     /gene="ftsI"
                     /locus_tag="ACHKJF_001830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP0949201.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30255..30620)
                     /gene="ftsL"
                     /locus_tag="ACHKJF_001831"
     CDS             complement(30255..30620)
                     /gene="ftsL"
                     /locus_tag="ACHKJF_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP0949202.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30617..31558)
                     /gene="rsmH"
                     /locus_tag="ACHKJF_001832"
     CDS             complement(30617..31558)
                     /gene="rsmH"
                     /locus_tag="ACHKJF_001832"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP0949203.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31560..32018)
                     /gene="mraZ"
                     /locus_tag="ACHKJF_001833"
     CDS             complement(31560..32018)
                     /gene="mraZ"
                     /locus_tag="ACHKJF_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP0949204.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32620..33624)
                     /gene="cra"
                     /locus_tag="ACHKJF_001834"
     CDS             complement(32620..33624)
                     /gene="cra"
                     /locus_tag="ACHKJF_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP0949205.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33706..33792)
                     /locus_tag="ACHKJF_001835"
     CDS             complement(33706..33792)
                     /locus_tag="ACHKJF_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP0949206.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33804..34295)
                     /gene="ilvN"
                     /locus_tag="ACHKJF_001836"
     CDS             complement(33804..34295)
                     /gene="ilvN"
                     /locus_tag="ACHKJF_001836"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0949207.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34298..36022)
                     /gene="ilvI"
                     /locus_tag="ACHKJF_001837"
     CDS             complement(34298..36022)
                     /gene="ilvI"
                     /locus_tag="ACHKJF_001837"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP0949208.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36141..36185
                     /locus_tag="ACHKJF_001838"
     CDS             36141..36185
                     /locus_tag="ACHKJF_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949209.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36340..37284)
                     /gene="leuO"
                     /locus_tag="ACHKJF_001839"
     CDS             complement(36340..37284)
                     /gene="leuO"
                     /locus_tag="ACHKJF_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP0949210.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLER
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9018 a        10444 c         9155 g         8833 t
ORIGIN      
        1 gagctttaca aacggtttct ttttaagcaa ctcatcttca accatgcata aagcgggtgc
       61 attcgctgcc gcataccatt attcttgatc tgacggaagt ctttttgtaa caattcaaac
      121 ttctttgatg taaacaaatt aatacaacaa acggaattgc aaacttacac acgcatcact
      181 gcgtagatca aaaaaacaac caccgcacga ggtttcatga tggaaggtca acagcacggc
      241 gagcagctaa agcgcggcct taaaaaccgc catattcagc ttatcgcgct gggtggcgcg
      301 atagggaccg ggttattcct gggtagcgcc tccgtaatac agtccgcagg gccagggatt
      361 atcctgggtt acgccattgc tggttttatc gcctttctga tcatgcgtca gctgggtgaa
      421 atggtggtcg aagaacctgt cgcaggctcc tttagccact ttgcttataa atactggggc
      481 agttttgccg gtttcgcctc tggctggaac tactgggtac tgtacgtttt agttgccatg
      541 gctgagctga ctgccgtggg taaatacatt cagttctggt atccggaaat ccccacctgg
      601 gtttctgccg ccgtattctt tgtggtgatt aacgccatca acctgaccaa cgttaaagtg
      661 tttggcgaga tggagttctg gtttgccatt atcaaagtta tcgcggtggt agcgatgatc
      721 atcttcggcg gctggctgct attcagtggc aacggcggcc cgcaggcgac cgttagcaac
      781 ctgtgggatc agggtggttt cctgccgcac ggcttcaccg ggctggtgat gatgatggcg
      841 attatcatgt tctcgttcgg tggtctggaa ctggtgggga tcaccgcagc agaagctgat
      901 aacccggagc aaagtatacc gaaagcaact aaccaggtta tctaccgcat cctgattttc
      961 tatattggtt cgttagccgt tctgctctca ctgatgccgt ggacccgcgt taccgccgat
     1021 accagtccgt ttgtgctgat cttccacgag ttaggcgata cctttgtggc gaatgcgctg
     1081 aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca gctgcgtata ttgcaacagc
     1141 cgtatgctgt ttggtctggc acaacagggt aatgcgccaa aagcgctggc gtctgtcgat
     1201 aaacgtggtg taccagtaaa taccattctg gtgtctgcac tggtaacggc gttgtgcgta
     1261 ctgattaact accttgcccc agagtccgct ttcggactgt taatggcgct ggtggtatct
     1321 gcactggtaa tcaactgggc gatgattagc ctggcgcata tgaaattccg tcgcgccaag
     1381 caggaacaag gcgtggtaac tcgcttccct gctctgcttt atccgctggg taactggatc
     1441 tgcctgctgt ttatggcggc ggtactggtg attatgctga tgaccccagg aatggcgatt
     1501 tcggtatacc tgatcccggt atggctgatc gtgttaggta tcggctatct gtttaaagag
     1561 aaaaccgcca aagccgtaaa agcgcattaa tctctctacg ccctcacccg tacagggtga
     1621 gggcaataat ctttacacca acgccccgta aatcgtcagt agtgcaatca ccaccacgac
     1681 cacgaacgag gttttcttcg ccattgaaac cgctgccttc ggcgtctcca ccttatcgac
     1741 atgcggttca cgcgccagag agaactgcgc cagacgcgtt aacacctgat actgcgaagt
     1801 atggaaatca cccagcgaag caaaccaggc cggtaacgct ttctcaccat gaccgatcaa
     1861 ggcatatacc acacccgcaa gacgaaccgg cacccaatcc agtacatgaa gcacggcatc
     1921 aatgccggac tgtaaacgat gatgcggcgt ctgatatcgc gccagccagt attgccatgc
     1981 acgcaaaaac gcatacccca tcagcgtaac gggtccccag gttcccccca caatcagcca
     2041 gaacagcggt gcaagataaa aacgaaagtt aatccacagc aatgcatttt gcagctcacg
     2101 caaatactca cgttcgtcgc agcctgccgg gacgccgtga atcatggtga gttcgccagc
     2161 catcgtggca cgggcatggc tatcattacg tgaagcagct gtcagataag catgataatg
     2221 aagacgaact ttacctgcgc caatacacag caaaccaatc agcagccaca ccagtagcgt
     2281 gggaacgttg aacaatactc cctgcaatgc gcgtaacagt aaaaaagtca cgcccatcgc
     2341 aataatggtc atgcctaacg tgcgcccgag agaaaaatgt ttcacccgcc gaaagaacgc
     2401 ttcaagacga tgatcaagct gccagtgctc gcccaactta aacaggcgct cgaaaattaa
     2461 caccagtaag gttgtaaata gcgtcatgtt gtctccttgc tgaccagcac acgaaaccgt
     2521 gcccaatcaa atgcaggacc gggatcggtt ttccgatccg gcgcaatatc acaatggccc
     2581 gtcatgtttt tagcgatatc cggatagcaa tcaatcagtg cccgcgtaac cgccgcaagc
     2641 tgttgatact gcgcatcggt atacgccagc gtatcggtgc cttcaagctc aatcccaata
     2701 gaaaaatcat tgcagcgttc gcgcccctga tactgagaga ctcccgcatg ccatgcacgt
     2761 ttatcgaaag gaacatactg gactatttca ccatcacggc gaatcaaaca gtgagcggag
     2821 acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct gcggatcaat agttccagtg
     2881 aataatgcgt cgatccacgg accgccaaac tcgcctggcg gcaggctaat attgtgcacc
     2941 accagcaggg tgggtgtttc gtcatccggg cggcaatcgt aatgtggtga gggaacgcgg
     3001 cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg atctccttat atgtggtgct
     3061 aatacccggt tcagagtagc atgtttctac cttatgattc gttagctatc tggagtttta
     3121 acatgccgcc tcgccgctat aaccctgaca cccgacgtga cgagctgctg gaacgcatta
     3181 atctcgatat ccccggcgcg gtggcccagg cgctgcggga agatttaggc ggaacagtcg
     3241 atgccaacaa tgatattacg gcaaaacttt taccggaaaa ttctcgctct catgccacgg
     3301 tgatcacccg cgagaatggc gtcttttgcg gcaaacgctg ggttgaagag gtgtttattc
     3361 aactggcagg cgacgatgtc accataatct ggcatgtgga tgacggcgat gtcatcaatg
     3421 ccaatcaatc cttgttcgaa cttgaaggcc catcccgcgt gctgttaacg ggcgaacgca
     3481 ctgcgcttaa ttttgtgcaa accctttcag gagttgccag taaggtacgc cactatgtcg
     3541 aattgctgga aggcaccaac acgcagttgt tggatacgcg caaaacctta cccggcctgc
     3601 gttcagctct gaaatacgcg gtactttgcg gcggcggagc gaatcaccgt ctggggcttt
     3661 ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc cggctcagtg cgccaggcgg
     3721 tcgaaaaagc gtcctggctg cacccggatg cgccagtaga agtcgaagta gagaatctgg
     3781 aagaacttga tgaagccctg aaagcaggag ccgatatcat catgctggat aacttcgaaa
     3841 cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa ggcgctactg gaagtgtctg
     3901 gcaacgtcac tgacaaaaca ctgcgtgaat ttgccgaaac gggcgtggac tttatctccg
     3961 tcggtgcgct aactaaacac gtacaagcac tcgacctttc aatgcgtttt cgctaatagt
     4021 tgacagattt atccgctcca tcgcgggcgg ataatctccc cttccccaac tttcttcgta
     4081 acgcctcgca aatttcttgt ctttcgctgc aacgaaataa aaaacctcgg aatctgcttt
     4141 cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt tgccaaagta gcgccaacca
     4201 aatcaaggag cgaaacagat ggacaagcaa cgcggtttta cacttatcga actgatggtg
     4261 gttattggca tcattgccat tttaagcgcc attggtattc ccgcttatca aaactacctg
     4321 cgcaaagccg cactcaccga catgctacaa acctttgtgc cttaccgtac cgccgtagag
     4381 ttgtgcgcgc tggaacatgg tggattagat acctgcgacg gtggcagcaa tggtattccc
     4441 tcgcctacca ccacccacta tgtttcagcc atgagtgtgg caaaaggcgt ggtgtcgctg
     4501 accgggcaag aaagtctcaa tggactaagc gtcgtcatga caccgggttg ggataacgca
     4561 aacggcgtca ccggctggac gcgcaactgc aatattcaaa gtgacagcgc attgcatcaa
     4621 gcctgcgaag atgtcttccg ctttgatgac gccaactaag gagcggcaat gaatattcca
     4681 cagctcactg ccctgtgtct gcgttatcat ggagtcttgc tggatgccag cgaagaggtg
     4741 gttcatgttg cggtagtcga tgcaccttcg catgagctac tggacgcatt gcatttcgct
     4801 accaccaaac gtattgagat cacctgctgg acgcgccaac aaatggaagg tcacgccagt
     4861 cgcacacaac agacattgcc cgtagctgtt caggagaagc atcagcccaa agcagagttg
     4921 ctaactcgaa cgttacaatc tgcgctggaa caacgcgcgt ctgatattca tatcgaacca
     4981 gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg tattgcatcc tttaccggat
     5041 gtttcaccgg atgccggagt cgcattaacc gccagattaa aagtgctggg aaacctggat
     5101 attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc
     5161 gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg
     5221 ttacagcagg tgagccaggc actggatgtc aacacgcttg gaatgcagcc gttacaactg
     5281 gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac tggccctaca
     5341 ggcagcggca aaacggtcac gctttatagt gccctgcaaa cgctgaatac cgctgacatt
     5401 aatatttgta gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa
     5461 atccatccgc gtgccggact cacctttcag ggcgtgttgc gtgcgttatt gcgccaggat
     5521 cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cagcagagat cgctattaaa
     5581 gcggcgcaaa ctggtcacct ggtgttgtct accctacaca ctaattccac ctgcgaaacg
     5641 ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc gcttacgctg
     5701 gtaatagccc agcgtctggt acgcaaactt tgcccacatt gtcgccggca gcaaggggag
     5761 cccatccaca ttccagacaa tgtatggcca tcgccgctgc cccactggca ggcacccggt
     5821 tgtgtacatt gctaccacgg tttttatggt cgtacggcct tatttgaagt tctgcccata
     5881 acgccggtca ttcgtcagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac
     5941 gcacgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt ggagcaaggc
     6001 ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcga gtaagcaact
     6061 ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga
     6121 gagccgtgct ttgctgctca tggcactaca gcaacagatg gttaccccac ttagcctgaa
     6181 gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca
     6241 actggcgacg ctactcaaag ccgggttaac gctttctgaa gggctggcac tgctggcgga
     6301 acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca
     6361 gggcattgct ttttccaatg ccttattacc ctggtcagag gtatttccgc cactctatca
     6421 ggcaatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc
     6481 gcgtcagcaa aaagcccagc gtcagttgac cgacaaagtg aaatcagcgt tacgttatcc
     6541 catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc
     6601 ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa cgcaggggat
     6661 catgacgctg gcagacttta gtggcgaatg gagctggctg ctggtgttgt tcggctttct
     6721 gctggcgata gccaataagt tgctgatgcg ccgaccgacc tggcttatag tgcggcagaa
     6781 attgctgtta cgcatcccga ttatgggttc actgatgcgg ggacaaaaac tcacgcagat
     6841 ttttacgatt ctggcgctga cacaaagtgc aggcattact tttttgcagg gcgtagagag
     6901 cgtcagagaa acaatgcgct gcccgtgctg ggtgcaactt ctgacacaaa tccagcacga
     6961 tatcagtaac ggtcatccca tctggctggc gctaaaaaat accggggagt ttagcccgct
     7021 ctgtttgcaa ttagtgagaa caggagaggc atccggctcg ctggacctca tgttagacaa
     7081 cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact
     7141 ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg tggcgatgta
     7201 tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc
     7261 tccacaccag cgttggaaga ttacaggttg ttgaagatgc ggttttcttg ttcctgcaca
     7321 cggataaatg tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa
     7381 gctgaacgca ggccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc
     7441 agcttaacgg ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc
     7501 atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg
     7561 atgcgaccgc cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca
     7621 ccgccgccaa aggcttttgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc
     7681 attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag ctgcggataa
     7741 ccgacgcctg ttttgacgcg agttgtacaa acagaacctg ggccaatgcc aactttaacg
     7801 atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac gttgccagca
     7861 caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg
     7921 gaataaccat tcgccacatc aatacaaacg aagtttaatg ccgggttcag gtcgagaatc
     7981 tgtttagttt tttcgaaatc cgcatcagac gtaccggtag aaaccatcac atgtttcagc
     8041 acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc
     8101 acagcagtca aaatatcaaa agaagccagc gcagaggcca tagaaaatgt acctacggtg
     8161 tccatatttg cggcgataat tggcacgccg gaccagctct gacctgaatg tttgaaggtg
     8221 aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg
     8281 agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg
     8341 ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg
     8401 cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa
     8461 atagtgagcg aacgtttcat caatttcgct ttcgtgcgct catttttaac gttttgataa
     8521 tcatgatgaa ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat
     8581 tggcagtggc aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga
     8641 tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc
     8701 tgatcacttt ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg
     8761 cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgacctgc tgcatccgct
     8821 gattcagcaa gagacgcaac accagatcca gcaagctact tccccctatg tactgtgggt
     8881 tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga
     8941 tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgagca
     9001 tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga
     9061 cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc
     9121 acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag
     9181 gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg
     9241 attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc
     9301 cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg
     9361 aaagaacttg accggcagca acgtaaactc cagacctgga ttggcgtgcc tggcgtggac
     9421 cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc
     9481 gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga
     9541 ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat
     9601 ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc
     9661 acccaggcac ttaccatggt gctggattta attcgccagt cggctccctt ccgtaaacaa
     9721 accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat
     9781 ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt
     9841 cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg
     9901 gcctgttgct aaggagtgaa aatgtcagaa actattacgg tgaattgccc aacctgcggg
     9961 aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag
    10021 ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt
    10081 tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga tgccgcacgt
    10141 aggcctgata agacgcggac agcgtcgcat caggcatctt gtgcaaatgt cggatgcggc
    10201 gtgaacgcct tatccgacct acagacgctt aagcttcgca attaccggtt cattggctgg
    10261 cggaaaatca tcggcattaa gaccgactag cgacatccac tcaccgggtt gcccttcttt
    10321 accccacggc tccccttccc agctttcgac cagccagaac cacagagtta tatgcctgtc
    10381 cgggaattca tattccagtt tttcaaatag cgaaaaatgt tgtggggtaa tcccgacttc
    10441 ttcctgaagt tcacgcacca ccgcctgttc cggcgtttcg cccatttcaa ttttaccgcc
    10501 gggaaactcc agtttattcg ccatgtgcgc atctgctgcg cgacgcgtta taaagatttc
    10561 attgttctcg ttgcgaataa tacctaccgc aatttgcagc tttttcattg tcttaaacct
    10621 ataaaaaagg cgcagaatcc tgcgcctttt acttcaactg ttagctttta ttgcaggcga
    10681 ccatggcact gcttgtattt tttaccagaa ccgcacgggc aaggatcgtt acgtcctact
    10741 ttgcgctcac cggtttgcgc cgccagtgca gctgcggctg cagagtcgtc atcctgatgg
    10801 ctaagctgct gcatttgcgc taaacgctcg gcttccatac gacgctgttg ttccagctcc
    10861 tcaacctctt caggcatacg tacctggact ttgctcagcg tgctgataac ttcatatttt
    10921 aacgactcca gcatcgctgc aaacatggag aacgattcac gtttgtattc ctgcttcgga
    10981 tctttctgtg catagccacg caggtggata ccctgacgca gatagtccat cgccgccagg
    11041 tgctctttcc acagagagtc gagagtttgc agcatgacgc ctttttcgaa gtgacgcatc
    11101 atctcagcac caaccacttc ttctttacgc tgatacactt cgatggactg cgccagaatt
    11161 cgctcacgca gcgtctcttc atgcagttct ggttctttat ccagccactc ggcaattggc
    11221 aaatcgaggt cgaaatcgtt cttcagacgt tcctgcagcc ccggaatatc ccacatttct
    11281 tccagtgact gtggcggaat gtaggcatca atggtcgctt tgaacacatc ttcacgaatg
    11341 ctgttgatgg tttcgctcac atcgctgaca tccagcagtt cgttacgctg ggagtaaatg
    11401 gcgcgacgct gatcgttagc cacgtcatca tattccagca gttgcttacg aatgtcgaag
    11461 ttacggcttt caactttacg ctgggcgttg gcaatcgctt tagtcaccca cgggtgttca
    11521 atggcttcgc ctggcttcat acccagttta cgcatcatgc cggatactcg gtcggaagca
    11581 aaaatacgca tcagcgcatc ttccatcgac aggtagaaac gggaagaacc agcatccccc
    11641 tgacgaccag aacgaccgcg caactggtta tcgatacgac gggattcgtg acgctcggta
    11701 ccgatgatat gcaggccacc tgcttccagt accgcatcgt gacgtacctg ccagtcggct
    11761 ttaatttttt caatttgctc tacggtcgga ttttccagcg cggcaacttc tgcctgccag
    11821 ctaccaccga gcacaatatc tgtaccacga cccgccatat tggtcgcgat agtcaccgca
    11881 gccggataac ctgcctgagc aacaatcgcc gcttcgttgg cgtggaattt ggcgttcagg
    11941 acgttgtgct taataccggc tttggtcagt tcgtttgaca ccagctccga tttttcgatg
    12001 gagatagtac ccaccagcac cggctggcct ttcgcagtac gttctttgat atcttcaatg
    12061 atcgcctgaa ttttttccgc ttcagtcatg tagaccaggt ccggcagatc tttacgaatc
    12121 attggacggt tggtcggaac aacgacggta tccagcttgt agatggagct aaattcgaaa
    12181 gcttcggtat cagcagtacc ggtcatcccc gccagttttt catacagacg gaagtagttc
    12241 tggaaggtga tcgaagccag cgtttggttt tcgttctgga tctgcacacc ttctttcgct
    12301 tccacagcct ggtgcagacc atcggaccag cgacggccct gcatggtacg accggtgtgt
    12361 tcgtcaacga tgataacttc accatcttta acgatgtagt cgacgtcgcg ggtaaacagc
    12421 gcatgagcgc gcagcgccgc cgttacgtgg tgcatcagca tgatgttggc cggagagtac
    12481 agagactccc cttcatccat gatgccctct ttcaccagca gttcttcaat cagcaccaga
    12541 ccacgttcgg tcaggttcac ctggcgagat ttttcgtcca ccgagaagtg gccttcgccc
    12601 tggaaggttt cggagtcttc tttttcctga cggatcaggt gcggaataat tttattcacg
    12661 cgtttataca tttccgagct gtcttctgcc gggccggaaa tgatcagcgg tgtacgcgct
    12721 tcatcgatca ggatggagtc cacttcgtcc accagcgcat agtgcagttt acgctgtaca
    12781 cgttcttcag ggctgaacgc catattgtcg cgcaggtagt caaagccgta ttcgttgttc
    12841 gtaccgtaag tgatgtcagc tgcgtaagct tcgcgctttg ccggtgctgg catgcccggc
    12901 aggttgatac cgacagtcag gccaaggaat tcaaacagcg gacggttgtt ttcggcgtca
    12961 cgttgcgcca ggtagtcgtt gacggtaact acgtgcacgc ctttaccggt tagtgcgttc
    13021 aggtaagcag gcagcgttgc ggtcagggtt ttaccttcac cggtacgcat ttcggcgatg
    13081 cagcgttcgt taagaaccat accgccgagt aactgaacgt cgaagtgacg cataccaaag
    13141 acgcgcttac ttgcctcacg taccacggcg aaagcttccg ggatcagatt ttccagcact
    13201 tcgccttttt ccagacgtgc acgaaactct gcggttttcc ctttcagttc ttcgtcggag
    13261 agtttttcca tctccggttc catggcattg atgatgttga ccactttgcg catccggcgc
    13321 agggtgcgat cgttacgact accgaaaact ttagttaaca atttgattag cataataaaa
    13381 tctcaaacgc cccgcgttgc ggagttaata aaatgaagta aaggtttatt gttgttaggt
    13441 gaggcgttga gggccagcac ggatgccttg cgcctggctt atccagacgg gcgtgctgaa
    13501 ttttgcttgt ggggtaaaat gcgcataatc aatgcgataa cccttttcag acggcgtgcc
    13561 ttcctgggtc agcagcgcgc tgagcgtatc cagtaatgca agatgttgcg cctgaagagg
    13621 caaagattct tcagcaacgg gcagtgtttg cggtgccatt gcgaaagaaa gatgacggat
    13681 taccgtgcga atggcatgtt gatgccagta atcaacggaa tagttcgaat tcgggcggcg
    13741 tgtgttcgct tccagcaagg ccaattgacc aaagttaact ttggctgaag gctcgtggtt
    13801 gcgggttgtc gcttttgcgg gcgcgtttgg ttcggcggcg ttgctgagcg caggcaaacc
    13861 taaactcgcc gcaaccatcc ctaataagag atgcggccag aagtagcgtt taccaaactg
    13921 tcgccagcgc gtcagtattc cactcacgtt attgccatcc cgatcgtatt caaacgtatt
    13981 tagcgcacaa atcttatcat taaagcctgc agtccacagc acgaatgaag ataagttgtg
    14041 cggaaaagtg cttaagaaaa cagcgttcgc accgtttttt atagctctta taaaattcaa
    14101 aaaaaaacga ctggttcacc tggccggaga gagtgccaga tttgccagtc gaattttata
    14161 cgacagtata aatgtcgtta tgccagtaca gctgaaggcg ctttgaaggc caacggcagt
    14221 tctgcgtcgt cctggaaggt cacatattcc caggcttcct gtttcgccaa gacagcctgc
    14281 agcagtttgt tattcagtgc atgaccggat ttataagcgg taaatgcacc aataatattg
    14341 tgaccacaca tgaacaagtc accgatcgca tcgagcattt tgtgacgcac aaattcgtct
    14401 tcaaaacgca ggccgtcttc gttcagtacg cgataatcgt caacaacgat ggcacaatcg
    14461 aagctgccgc ccaggcacaa accacgggac tgcagatatt cgatatcacg catgaaaccg
    14521 aacgtacgcg cacggctgat ctggcgcata aacgcatcag cggagaagtt catcgcatag
    14581 cgctggttgc tggaatcaat agccggatgg ttaaaatcga tggtgaaatc cagcgaaaaa
    14641 ccattgtacg gcttaaattc agcccactta tcgccatctt cgacacgaac agtctctttg
    14701 atgcgaacaa attttttggc gcagttcaac tcgtcgatac cggcgtcaag cagcaggtat
    14761 acaaacggag cggcgctgcc gtccatgatc gggatttccg gcgcgttaac ttcgataaca
    14821 atgttatcga tgcccaagcc cgcgagagca gcattgaggt gctctacggt tgaaatccgt
    14881 acatcatgct cgttgaccag acacgtacag agcatggtat cacgcacaga tttggcatcg
    14941 gccgggaaat ctaccggtgg attcaagtcg gtgcgacgat agatgacccc ggtgttggcc
    15001 ggcgcagggc gtaacgtcag ggtgactttc ttgccggtat gtaaaccgac acccgtcgcc
    15061 tgaacgatac gtttaagtgt cctttgtttg atcatcgtat tatctcgcca aattacctat
    15121 ccaaccgaag tgtactatac attcggcggg ccagtttagc acaaagagcc tcgaaaccca
    15181 aattccagtc aattcttaat cagcttgctt acgcaggaat gctgggatat ccagataatc
    15241 cggctctttc gcagtttgcg gcgcattgtc attcacgact ttagcaaccg gcttctgctc
    15301 ctgggtcagc ggagccatcc catgctgctg gtagcgatcc atcactggct gctgaacctg
    15361 cttattggtc accagagtga tttcaggacg tttgtccatg ccgatacctg tcgcaacaac
    15421 ggttacgcgc agctcgtcat tcatatccgg gtcaagagaa gtaccgataa ccacagtcgc
    15481 gttgtcggaa gcaaatgcac ggatggtgtt acctaccgtt tcgaactcat ccagacgcag
    15541 gtcgaagccc gccgtgatgt taaccagcac gccgcgcgcg ccagacaggt cgatatcttc
    15601 cagcagcgga gaagagatag ccatttcagc agcttcttcc gcacggtctt caccgctcgc
    15661 cacgccagaa cccatcattg cgtagcccat ctcagacatt acggtgcgta cgtctgcaaa
    15721 gtccacgttc atcaaacccg gacgagtaat cagttcagcg ataccttgca cagcgccttt
    15781 cagtacatcg ttcgctgcgc caaacgcatc cagcagggag ataccgcggc ccagaacttt
    15841 cagcagtttg tcgttcggga tagtgatcag agagtccaca tgcttggaca gttcagtgat
    15901 cccctgctcc gcgaatgcca tacgcttctt gccttcaaag ttgaaaggct tagtgacgac
    15961 agcaacggtc aggataccca aatcttttgc cacttcagcg acgactggtg ctgcacctgt
    16021 accggtacca ccacccatac ccgcagcaat aaagaccatg tctgcacctt ccagcgccgc
    16081 acgcaatgca tcgcgatcct catcagccgc attgcggcca acttctggat tagcgccagc
    16141 gcccagtcct ttggtgatac cgctaccgat ttgaatcgtc tgtccaaccg ctgttttacg
    16201 cagcgcttgt gcatcggtat ttaccgcgaa gaattcaaca ccttcaatgc gctcgcgcac
    16261 catgtgttca acagcattac cgccgccgcc gccgacgccg atgactttaa tcaccgcgtc
    16321 attggtaagt tccattggtt caaacatagt ttctctccga tttgtgcctg tcgcctgagg
    16381 ccgtaatcat cgtcggcctc ataaaaatta aaactctttt cgcagccaac tattgagtcg
    16441 cttgatccac gagccaactg atgctgtaac acgtttttct acttcagctt caccgttaag
    16501 atgtgactct ttcccatagt gaagcaatcc caccgccgtc gaataatacg gctcctgagc
    16561 ataatccgtt aaaccggtaa tgttcagcgg cgcgccgata cgcacttgcg tatgaaacac
    16621 gcgctgagca caggctgcaa gaccttcgat ctgcgctgcg ccaccggtta atacaatgcc
    16681 tgccgccagg tgatgtttaa ccccttgttg gcgaagcttt tcctgcaact gcaatatctc
    16741 ttcgttgacc aggttgagca gctcggtata gcgcggctcg atcacctctg ccagggtctg
    16801 acgttgcaga ctccgcggcg gacgaccgcc tacgctcggc acttccacgc tctcatcttt
    16861 tccaacgatg gaacccagcg cacaaccgtg gcgaacttta atcgcttcgg cgtcgcttgg
    16921 cggcgtgcca aaggcgtaag cgatatcact ggtcacgaca ttgccagcat aaggaattac
    16981 cttagtgtgg cgcaatgccc caccggtata aacggcgata tccattgtac caccaccgat
    17041 atcgacgacg cagacaccca gttcacgttc atcttccgtc aataccgaat aacttgatgc
    17101 cagtccggca aatatcagtt ggtcaacttt cagtccacaa cgttcaaccg ctttgacgat
    17161 gtttttcgcc atatcgttgt gacatgtgat caggtgcact tttgcctgca tcctcacgcc
    17221 cgaaagtcct accggattct tgatcccttc ctgatagtca atcgcatact cttgcgggat
    17281 cacatgcagc acacgatgct catcgcgcac acgcaccgat ttcgcggtat ggacgacgtt
    17341 ttccacatct tcttgcgtca cttcttcttc agaaataggc accataccaa tttcattctg
    17401 gcagctgatg tgcttaccag aaagcgccag atataccgaa gagatctgac aatctgccat
    17461 caattctgcc tggtcaatgg cacgttgtac gcacttgacc acggattcga ggtcgttcac
    17521 cccgccttta tccataccac gcgacgggca gctgcccacg ccaatgatat tgaccatacc
    17581 gtcgggcaga acttccccta ctaaagcggc aaccttcgcg gtaccaatct ccagtcctac
    17641 taccagtttt ctgtccgtcg ccttgatcat tgttgttctg cctgtgcctg attttgttgc
    17701 tgagtagatt cctctggcgg caagggcgcc cagcctactg ccgctccaga gtcataacgc
    17761 aaatcaacgt agctaatccg tttgccatcg gtttgcgcct gctgctgtaa aaccggataa
    17821 agttctacaa agcgagccaa acgtttcatc gtatcgcccc ggccaagatt gagcttaata
    17881 tcgttattca gcgtcaactg ccaggaacgc cgcgcggtca tcgccgcttc cttcagagta
    17941 aatctgtcct ttgccagcat ctgccccatt tcgcgatagc cctgcaacac ttcattggcg
    18001 ctgccttccg ggccatacag cattggaagc acctgcttgc tggtgcgatc tggcggcacg
    18061 ctgaaggtgt ttccttccgc gtctaccata tgttgatcat tccaccgcgc aatcggcgca
    18121 tattcaacca gatgaatctt caattcatca ggccactgct ttctgacgct cacctgctta
    18181 atccacggca ggcgttgttc tatttgcgtc tgaatgatgt tgacatcctg ggtcataaag
    18241 gtacccggct cacccaatgc caggatcgac tgccggatat cgtcattacg cgtgtaatgg
    18301 cgttcaccgg tcaacaccag ctttgagagc ggcaggcgtt gcgcatcttc catccagccc
    18361 aacacgaccc agccgctcac caacactgtc gttaaaacgg tcagcaggaa aaggatcccc
    18421 gccagacgcg ttccattatt gcggcgagaa gaaacctctt cttcactgtt tcgcgtgttc
    18481 agagcagcct gcgacatatt agtccgccag ttccagaatt cgtactacca actgcgagaa
    18541 gctcatacct gcctgacgtg ccgccatcgg caccaggctg tggctggtca tacccggtga
    18601 ggtattggct tccagcagat aaaactgtcc atcgctgtcc agcataacgt caatacgtcc
    18661 ccatcctttg caacctaacg tcgtccatgc tttcagcact aatgcctgca aattggcctc
    18721 ttgtgacgct tctagacctg cggggcagaa atactgtgtc tcatcagaga gatacttcgc
    18781 ctcataatca tagaaggttc cggacggttg aatacgtatt gacggtaaaa tttcttcacc
    18841 gagtatcgca accgtgaact ccggcccact tagccatttt tcaatcaata cttcttcatc
    18901 gtgctgaaat gccaatctta atgcatcttg tagagcattt tctgctacta cttttgacat
    18961 tcccacactg gaaccttcgc ggctcggctt aacgataacc ggcaaaccca gagcagaaat
    19021 ttctgctaac tgcttatcac tcaggccttt ttcaaactct gcgcgggtta acgctaccca
    19081 cggcgcgacc ggtaaaccgg caccttgcca cagaagtttg ctgcgtagtt tatccattga
    19141 aagcgcagat gccatcactc cgctgccggt ataaggcaag cccatcagct cgagcatgcc
    19201 ctgcagcgtg ccatcttcac cgccgcgacc gtgtagcgcg ataaacactt tctgaaagcc
    19261 catcgacttc agttgcgtca cgtcgacttc tttcgggtcg acaggatacg catcaatacc
    19321 gccttcacgc agcccggcta acaccgctgc gccagaattc agagaaactt cccgctcagc
    19381 ggaagtcccg cccaacagga ccgcgatttt atcagtcatg ttgttcttcc tccggagttt
    19441 gcggcttcag tttgatttca gctaaagaac gggcaatttt tccgatatta ccagccccct
    19501 gaacgagaat caggtcgtta ccggttaata ccggtgccag catctcggct acctgcgcag
    19561 ggtccggcac cagaatggga tcaattttcc cacgtccacg aattgtgcga cacagcgaac
    19621 ggctgtccgc tcccggaatt ggcgcttcgc cagccggata cacttccagc atcaacaggg
    19681 tatcaacctg cgtcagcaca ttggcgaaat catcatacag gtcgcgcgta cgggtaaaac
    19741 ggtgcggctg aaacagcatt accaggtttt tatccggcca gcctgcgcgc gccgctttaa
    19801 tggtggcgtc aacttccgtc gggtggtggc cgtagtcatc gaccagcatt gccgtaccgc
    19861 ttttaccatt cactggctcc agcgggaatt caccgaggaa atcaaaacgg cgcccagtcc
    19921 cctggaagct ctccagcgca cgtaaaatag cctcgtcatc aattccctct tccgtagcaa
    19981 ccgcaaccgc agctgcggcg ttcagcgcgt tatgacgacc tggcgcattc agggtgacgc
    20041 gcatcggctc tttgtcctgg cgcagcagcg taaagtgccc ctgcgggcca atctgctgat
    20101 aatcttctac acgcacgtcg gcatcttcgc tgaagccgta agtcgtggtc tgacgtccca
    20161 ctcgcggtaa caattcgcgg atcaccggat catcaacaca catcaccgca cgaccgtaaa
    20221 acggcaggtt gtgcagaaaa ttaataaaag tctgttttaa attctcaaag tcgccctggt
    20281 aggtatccat gtggtcggct tcgatattgg tgacaatcgc caccatcggt tgcagatgca
    20341 ggaacgatgc atcactctca tctgcttcgg caatcaggta ccgaccatgc cccaaacgcg
    20401 catgaacccc cgccgctttt accagcccgc cgttaacgaa ggttgggtcg agccccgctt
    20461 ctgcgtagat gctggaaacc atcgcggtgg ttgtcgtttt gccgtgcgtt cctgcaatgg
    20521 cgatgccatg acgaaaacgc attaactcag ccagcatttc ggcacgacgg atcaccggaa
    20581 tacgcgcttc atgagctgcg acaatttccg ggttatcggc agaaatggcg ctggaaacaa
    20641 cgaccacgct ggcatcacgt acgttttccg ggcgatggtt gaaataaatc gtcgctccca
    20701 gattcattaa ctgctgcgtg accggatttg gcgctaaatc ggaaccactg atctgataac
    20761 cttcattggc cagaacttcg gcaataccgc ccataccggc accaccgatg ccgacaaaat
    20821 gtatgtgccg aacgcgacgc atttcgggca cgatggaacg cagttttgcc aattgttgtg
    20881 tattcattct ttacgccatt aacttcttaa attcatacga tgcaaaaggc atcgctacaa
    20941 ttacgcccgg gcagcccggc tcacttcatt tgccactcgc tcggtggcat ccggaatgga
    21001 tgcagcgcgg gcgcgttctg ccatggttaa taaggtttct cgcgaccacc cggccagggt
    21061 gttggcgaca gcatccacgc taagctgtgg ctgctcgata attttggctg cgcccgcttt
    21121 ttccagcggt agcgcattcc agtattgctg gcggtcttta tgttgaaacg gcacaaacaa
    21181 cgccggtagt cctgccgcgg cgatttcact caccgttaaa gcaccggagc ggcaaacgac
    21241 gacatccgcc cacgcatacg ccgccgccat atcatcaata aattccgtca ctttatgctg
    21301 cggttgcccc gcttcggcat acgcctgttc aacggattgt tgcgaacctt taccgctctg
    21361 atgccagata gtgactgaat cacccagttt cgcagcaacc tgcggcattg tctggttaag
    21421 aatgcgtgcg ccctgggaac cacccactac cagcacacga accggacctt cacgtccagc
    21481 caaacgttgc tgcggcaacg gcagcgccag cacatcggta cgcaccgggt tacccaccac
    21541 ttccgcatta gggaaagcgc ctggaaacgc ctgcatcact ttggtggcaa tcttcgccag
    21601 ccatttattg gttaagcccg caataccgtt ttgttcatga agtacaaccg gaatgcctaa
    21661 cgaccacgcg gccagaccac ctggacctga tacatagccg cccataccga gtaccacgtc
    21721 aggtttgtac gctttcataa tcgcccgcgc ctgacgccag gcgttgaaga tacgcagcgg
    21781 cgcagctatc agtgctttta taccttttcc acgcagacca gagatacgaa tgaaatcaat
    21841 ttcgatgcca tgttttggca ctaagtccgc ttccatacgg tcggcagtcc ccagccagcg
    21901 aacttgccaa ccctgagcca ttagatggtg cgcaaccgcc agtcccggga atacatgtcc
    21961 accggttccg cctgccatca ccattaatcg ctttccttga ccactcatcg tgaacctcgt
    22021 acaaacgcct gcgctttctc cagacgcgtt tcataatcaa tacgcaacag catcatgatg
    22081 gctgtcgaca taatcagtaa gctcgaacca ccgtaactga tcagcggcaa tgtcagacct
    22141 ttggtcggta acatccccgc cgccgcgcca acgttaacca gcgcctggaa gctaaaccag
    22201 atgccaatag aacaggcgag aaaaccggaa aaacggtggt caatttctaa cgctttgcgg
    22261 ccaatcgaca tcgcgcgaaa agcgacgaag aataccatta aaagtgccag caccacaccg
    22321 acatacccca gttcttcgcc gataatggcg aaaataaagt cagtgtgcgc ttccggcaga
    22381 tactccagtt tttgtaccga gttacctaaa ccttgtcccc aaagttcgcc gcgaccaaac
    22441 gccatcagcg attgcgttaa ctgatagccg ctgccaaagg gatcttccca cgggttccag
    22501 aatgcggtaa cacggcggat acggtacggt tcggcgagta tcagcaacac aaccgctgaa
    22561 atgcccatac cgataatggc aatgaactgc cacaatttcg ctcccgccag gaacaacatc
    22621 gccagcgtag tcacaaacaa caccaccacc gtaccaaggt ctggctgtgc cagcagtaac
    22681 actgccaaca ccagaatcac gcccatcggt ttcaggaagc cgcgcaggtt attacgtact
    22741 tcgtcgcctt tacgcaccag atagttggcg atataacaaa acagcgacag ttttgtcagc
    22801 tccgcaggct ggatacgcag caaaccgaga tcgatccaac gcgatgcccc tttaaccgag
    22861 ctacccacta ccagcacgat catcagcagg atgatagatc cgagcagcat cgtggcactg
    22921 tagcgttgcc agaactccat cggcagacgc agcgtaatga tcgccagaat gaatgccagg
    22981 atcagataga cgccatcacg cttcgcgaag aagaacggat cgttggttaa gcgttgccct
    23041 atgggcattg acgccgaggt caccatgata aagccaatcg ccgcaaggcc gaaggtcagc
    23101 cacagtaagg tgcgatcgta catgatcagg ctgtcggtat ctttttcccg cgagcccatc
    23161 acccagcctt ttagcgccgt ggagatccag accaggatac tgaatcctgg caggcgcggc
    23221 attttcaggc gagggagaga taaacgcatc aacctaactc cttcgccaga cgggcaaact
    23281 cattgcctcg ttgttcaaag ttcttgaact gatcaaggct ggcacaggcc ggggagagca
    23341 gaaccatatc gcccggctga acacgcggag ccagcaagcg catcgcctgt tccatagttt
    23401 cggtttgttc tgccacttcc gggcgtagcg ccgccagctg cgcaccgtca cgaccaaaac
    23461 agtacaaacg cacgttgtcg ccattcagat agcgcgccag tggactgaag tccgccgatt
    23521 taccatcgcc gcccagcaac aaatgcagcg tgccgtctac ctgcaagcca ttcaacgccg
    23581 cttccgtact gccgacgtta gtggctttcg aatcgttaat ccagcgtacg ccattgtgct
    23641 ccagcaccac ttcaaagcga tgcggcagac cggtgaaggt ggttaacgct ttcaggctgc
    23701 tggcgcgcgg caaaccagct gcgtctgcca gtgccagtgc tgccagcgca ttggtgtagt
    23761 tatgctgccc ggaaagtttc atctctttca cgttcagcac tttctcgcct ttcactcgca
    23821 gccaggtttc tccctgctga tgattcaggt gatagtcacc catgttgacg ccaaagctga
    23881 cgcagcgttc atcagcaccg cgaatcggca ttgttaaggc atcatcagca ttgacgacac
    23941 aaactttcgc attttcgtaa atacgcaatt ttgccgcacg gtattgctgc aaaccaaacg
    24001 gatagcggtc catatgatct tcagtcacgt tcagaatggt cgccgccacc gcctgtaagc
    24061 tggaagtggt ttccagctgg aagctcgaca gttccagcac gtacagttcg cactcagcat
    24121 caagcagcat caacgctggc aggccaatat tgccacccac accaacgtta acccccgccg
    24181 ctttcgccat ttcacccact agcgtggtga ccgtgctttt gccgttagaa ccggtaatcg
    24241 ccacaatcgg tgcttgtgct tcgcgacaga acagctcgat atcgccaacg atttcgattc
    24301 cggcatcagc ggcagcgctt aaggatggat gcgccagtgc aataccggga ctggcgacaa
    24361 tcagatctgc cgccatcagc cattcatcat tcagactgcc cgtgtggcgt tctacggctt
    24421 cgggtaattt atccaggcca ggcggtgtca tacgcgtatc cataacgcgc ggcgtcacac
    24481 cgcgagcgag gaaaaagtcc acgcaggaaa gcccggtgag gcccaggccg ataatgacga
    24541 catttttacc ctgataatca gccatgatta acgtaccttc agcgttgcca gaccaatcag
    24601 aaccagcatc agcgaaataa tccagaaacg cacaatgacg cgcggttccg gccagccttt
    24661 cagttcatag tggtgatgaa tcggtgccat gcggaaaata cgttgtccgc gcagtttaaa
    24721 ggagccgacc tgcaggatga cagaaagcgt ttctaccacg aacacgcccc ccataatcac
    24781 cagcaggaat tcctgacgta gcagtacggc gataatgcct aacgcaccac ctaacgccag
    24841 cgaacctaca tcgcccataa agacctgcgc cggataggtg ttaaaccaca ggaagcccag
    24901 tcctgccccg actatcgccg tgcagacaat gaccagttcc cctgcgtgtc gcagatacgg
    24961 tatatgcaag tagctggcaa agttcatatt gccggtcgcc cacgccacca gcgcaaaacc
    25021 accggcgaca aatacggtcg gcataattgc caggccgtcg agaccatcgg tcaggtttac
    25081 cgcgttgcca gtacccacaa tgacgaagta agccagcaga atgtagaaca gccccagctg
    25141 cggcatcaca tctttaaaga atgggaccac cagctgcgtt gcgggcgtgt ctttgccggc
    25201 aaggtacagg gcgaaggcga cacccagcgc aatgaccgac atccagaaat acttccaacg
    25261 agcgatcaac cctttggtgt ctttacgcac cactttgcga taatcatcaa caaagccaat
    25321 aacaccgtaa cctaccagca ccaccaacac gcaccagacg tacggattgg acgggtaagc
    25381 ccacagcagt acggagatca caatcgccgt caggatcata atcccgccca tggtcggcgt
    25441 accgcgcttg ctgaagtgtg attcaggacc gtcgttacgc accacctgac caaaggaaag
    25501 tttttgcaaa tgagcaatca tacgcgggcc catccacaat gagatgaaca gcgcggtcag
    25561 caggctgacg atggcgcgaa acgtcagata ggaaaagacg ttaaagccgg aataatattt
    25621 gaccaaatgt tcggccagcc aaactaacat gtcccattct cctgtaatgc gcgtactacc
    25681 tcttccatgg cggcactacg tgaaccctta actaaaatcg taattacctg ttgctcagca
    25741 atcagtgatt taagacgcgt aattaacgca gttttatcag caaaatgttc gccaacgccg
    25801 ctggcggtgc tgatagcatg gctttgttta cccacgctta acacgcggtc aataccagca
    25861 gcttttgccg cttcgcccac ctgtacatgg caggcttcgc tttcagcgcc cagttccgcc
    25921 atatcgccca ccaccagcac gcggtagccc ggcatttcag ccagtacctg aacagcggcg
    25981 gtcattgaac cgacattggc gttgtaggag tcgtcgagca gcaactggtt ttctgccagt
    26041 tggatgggga acagacggcc tggaacagct ttcagatttg ccagccccgc tttgatagca
    26101 tcaagcgttg cgcccacgga catggagagc gcagcggctg ccagcgcatt cgcaatattg
    26161 tgacgccccg gcaacggcag cagaacatcg acgctaccgg ttggggtttg tagggtaaat
    26221 tccgtaccgt gcgaggtcac atggatattg gtggcggtga aatcgctgtt ggcggcattg
    26281 ggtgagaaac gccacacttt gcgtgagcca attacgctct gccagttcag ccagtcgttg
    26341 ttgtcggcgt tcataatggc gataccgttt tccggcaggc cgctaaagat ttcacctttc
    26401 gctttcgcga cacccgcaag cgagccaaaa ccttccagat gcgccgctgc caggttgttg
    26461 accagcgcag cttccgggcg agtcagactc acagtccagg ctatttcgcc ctgatggttc
    26521 gcgccaagtt caataactgc gtaattgtat tccggcgtta agcgcaacag cgtcatcggt
    26581 acaccgatgt cgttgttgag attgcctgcc gtataaagcg tgttgccgca ctggcttaaa
    26641 atcgccgccg tcatctcttt aacggaggtt ttgccggagg accccgtcag agcaaccacg
    26701 cgcgccggaa cttgctggcg aacccatgca gccagttcac caaacgccag acgcgtatcc
    26761 ttgacgatta actgcggcag gtcgatgtcc agcggacggc taaccagtag tgcgcctgcg
    26821 ccgccagctt tcgcctggtc ggcaaaatcg tgggcatcaa aacgttcgcc tttcagggca
    26881 acaaacaggc agcccggcgt cagttttcgg gtatcagtgg ttacagcatc aagggtgata
    26941 tctgcacctt gcagttcacc gttgagaatg tcggtaagtt ggctaagggt tacgctaatc
    27001 atgcaatcac ccccagcaga cgcgccaccg tgacgcgatc ggagtagtcc agacgctgat
    27061 tgccaacaat ctggtaatct tcatggcctt tgcccgcgac cagtaccaca tcattctctt
    27121 tagcctgcat aacggcgcaa gtcaccgctt cagcacggcc ttccatcact ttggcatgtc
    27181 cggcatctaa cattcccgcc agaatatcgt tgatgatggc acgcggttct tcggtacgcg
    27241 ggttatcgtc cgtcaccacc gccacgtcag caaactcttc ggcaattgcg cccatcagtg
    27301 gacgcttacc tttatcgcga tcgccaccac agccaaagac gcaccacagc ttgcccgcac
    27361 agtgcaggcg cgccgcctgt aaggcttttt ccagtgcatc cggcgtatgc gcgtaatcca
    27421 ccaccaccgt cggtttgcct ggcgcagtga acacttccat acgtccgcaa accggttgca
    27481 gacgcgcggc ggttttcagc agatcagcca gtggatagcc gagtgccagc agtgtcgcca
    27541 gcgcgagcag caggttgctg acgttaaaag cgcccatcag atggctttca atttcgccat
    27601 cgccccaact tgagctaaag cgaatcgtcg caccgctgtc gtgatagttc acgtcgatcg
    27661 cttttaacca gcgtccgtga cagttcggat tgatatgatc ttccattgat accgcaaccg
    27721 cgtccggcag ttttgccagc cagcggcggc ccacttcatc gtcggcgtta ataatcgcct
    27781 gaccgcaatg atgttcagaa taaagcagcc atttcgcagc ttcgtagtgt tccatatcac
    27841 catgataatc aaggtgatcg cggcttaagt tggtaaagac cgacgccgca aatttcaatg
    27901 ccgccacacg gtgctgtacc agcccgtggg aggaaacttc cattgcgcaa aacgtcgcgc
    27961 cctgctccac cagccccgcc agctcatgct gaacatcgac tgcggaaccg gtagtatttt
    28021 ctgtcgggat cactttccca agcagaccgt taccaacggt gcccattacc gcgctggttt
    28081 cgccaagcag ttggctccac tgcgccagca gctgggtagt cgtggttttg ccgttggtgc
    28141 ccgttacgcc tacgagacgt aaattgtcag agggttcatg gtaaaagcgg cccgccagtg
    28201 cagataaacg ctcgttgagc tggctgagat agatgaccgg tacgccgtgc atttcacgga
    28261 tttcaccgtc ggtcgcctca tctttcgcct ctgcaataat ggcagccaca ccttgcgcta
    28321 tcgcctgcgg gatatatcga cgcccgtccg cctgatgacc tactacagct acaaagagat
    28381 cgcccgccgc agccacacgg ctgtcgagtg tcatctctcg cagtgctcgc gaaggtgcgt
    28441 ctggcaccca cggagcaaga aggtcgcgca aattacgatc tgccacctgt cccctcgcct
    28501 tgattaatca caaattcatt tttatcgccc gttgtcagcg catccggctc gatgttcatg
    28561 gtacgcaata cgccgcccat gatggcacca aagaccggcg cggaaacggc gccgccgtag
    28621 tatttacccg cctgcggatc gttgataaca acaaccagcg cgaagcgcgg ctgactcgca
    28681 ggcgcaacgc ctgcggtata agcaatatat ttattgatgt agcgaccgtc cggcccgacc
    28741 tttttcgcgg taccggtttt aatggcgata cgatagcctt taatcgccgc tttcacgccg
    28801 ccgccgcctg gtagcgccac gctttccatc atatgcacca cggtgcgaac aatggattcc
    28861 gggaagacac gttcaccggg aaccggggga tcaactttgg taatcgacag tgggcgataa
    28921 atgccgtagc tgccgatagt tgcgtagact cgcgctaact gtaacggtgt taccattagc
    28981 ccgtagccga aagagaaggt ggccctctct atgtcagacc accgttgttt ttgaggatat
    29041 aagccactgc gttctccgac caaccccaaa ttggtcgctt ttcccagtcc aaaacgtgag
    29101 taagtatcta ctaacgctga ggacggcatc gctaacgcca gcttggaaac accgacgtta
    29161 ctcgacttct gtaatacccc ggttagggtt aattcgctgt agcgtgccac gtctttgatt
    29221 tcgtggccgt taattcgata aggaatggta ttgagtaccg agttttcccg caccacgcca
    29281 cgttgcaacg cggtcattac caccatcggt ttaaccgttg agcccggttc aaacacgtcg
    29341 gtgatggtac ggttacgcat cgcctctttc ggcgtgccgc tcagattgtt agggttgtat
    29401 gacgggctgt tagccatcgc cagcacttca ccggtgttga catccaccag cacggcgcta
    29461 ccggattcag ccttgttaaa ggccaccgcg ttgttcagtt cgcgataaac cagcgcctgc
    29521 aagcgttcat caatactcaa cgccaggttg tgcgccgcct ggctgtcagt agaagaaata
    29581 tcttcaatta cgcgaccata gcggtcttta cgcacaatgc gctcacccgg ctgcccggta
    29641 agccatttat cgaaactctt ctcaacgccc tcaatcccct gactatcgac gttagtaaag
    29701 ccgatgaggt gagcagtcac ttcgccggat ggataataac ggcgagattc ttcacgcaga
    29761 tgaatccccg gcagtttcag ttttttgatg tagtccgcca tgtcagggtt tacctgacgc
    29821 gccagataaa taaagcgccc tttcgggttg gcgttaatgc gggcagaaag ctgatccagc
    29881 ggaatattga gcgcgttagc cagcgccttc cagcggtcac cgacgctgat accgccagcg
    29941 tcatgcactt ctttcgggtc agcccaaatc gcttttaccg gcacgctcac cgctaacggg
    30001 cgaccagaac ggtcagtaat catgccgcgg gaggtggaaa cttgctgaac gcgaagagaa
    30061 cgcatgtcgc cctctttcac cagcatatcc ggggagataa cttgtaacca cgctacgcgt
    30121 ccgagcagaa aagccagcgc caggagaata cagccgcata acaacgcaaa acgccaacta
    30181 ataaagttgg catgttcttc ctgacgtttt ggtttctgcg ttttcgccgc tgctttcatg
    30241 cgtcgcgttt atccttattt ttgcactacg atattttctt gtgacggatc aacatgctgc
    30301 atttgcagct tttccgtggc gatcctttcc acccggctat ggtcgccgag cgcattctct
    30361 tcaaggatca ggttgcgcca ttcaatgtct aaagcatctc gctccagcac cagttgttcg
    30421 cgctgagcgg tcagtaaacg ggtatggtgc gccgtggtta ccacggtcac cgccgtcaaa
    30481 ataatgcaaa tgaacaggca gagtggcagc ttcccaaatc gcaaaagatc gtcaccgata
    30541 acaccaggca atgcatggcg ctcgtggctt cccatcgatc ctttaacttt gcttagagct
    30601 tctgtcactc tgctgatcat gcgttcgtcc tctctgcgat acgcagaact gaactacggg
    30661 cacgaggatt ctcagccacc tcttcttcgc ccggcattaa cttgcctaat gctcgcagct
    30721 gacggccacc cagttttttg agctgctctt cagtcatcgg taaccctgcc ggaacttgcg
    30781 gaccgcggct gttttcacgc ataaaacgtt tcacaatacg gtcttccagc gagtggaagc
    30841 tgatgatcga aagccgccca cccggggcca gcacgttgag cgagcttttt agcgcctgct
    30901 ctatctcctc cagttcactg tttacccaaa tgcgcaccgc ctggaaggta cgagtcgcgg
    30961 gatgtttaaa cttatctttc accggcgttg cagcagccac gacttccgcc agttctttgg
    31021 tgcgggtcat cggctgttcg cggttacgct cgacaatggc gcgggcaatg cgtttggcaa
    31081 aacgctcttc accataggtt ttcaataccc aggcgatatc ggcttcttct gcggtttgta
    31141 gccattcagc ggctgactgc ccacgggttg ggtccatacg catgtccagc ggaccatcgc
    31201 gcataaagga aaagccgcgc tcagcatcat caagttgcgg tgaagagacg ccaagatcga
    31261 ggagaatgcc gtcgatcttg ccgataagat cgcgctcggc aacgtattcg cccagcgcgg
    31321 agaaaggtcc gtggatgatg gagaagcgcg gatcatcaat agtcttcgca acggcgatag
    31381 cctgcgggtc gcgatcgatc gccagcaaac gcccctcttc gccaagctgc gagaggatca
    31441 gacgtgagtg accaccgcga ccaaaagtcc catcaatgta gatgccatca ggacggatat
    31501 tgaggccatt aacggcttca tccagcagca ccgtagtatg tttatagttt tccatcattt
    31561 tatagagaca agtcctgcag tcgctccgat aagtctccgg tagccaactg ctctgcgtcg
    31621 atatcttcct tgacctgttg atgccaggtt gtttcatccc acagctcaaa cttgttgaac
    31681 tgtccaacca gcatcacttc ttttgtcagc ccggcatgtt gccgcagtac tggcgcgatt
    31741 aacaatcgac ctgcgccatc catctgacat tcgctggcat gacctaacag taggcgctgc
    31801 acacggcgct caaccgggtt catgctcgac agacgcgata atttttgctc gataatttcc
    31861 cattcaggca gggggtaaag cagcaggcac gggtgatgaa tgtcaatggt gcaaaccatt
    31921 tgaccggcag cgttctcaag cagctgttcc cggtaacggg taggcactga taagcgccct
    31981 ttgctgtcga gattgactaa cgttgctccc cggaacatgc cagcctcacc ccttattccc
    32041 actttgcccc acaatttccc actgaaagga gtttacggag cggaggaaaa gcttgtcaag
    32101 ccagtcacaa ggcataccga ggtaaaaaac ccgatgttta cggctaatat cagcgttgtt
    32161 tattttacgt ccaacgtgcg cagcaaatta acgttatgaa tagctgtaag aaaaaatccc
    32221 tcatcgtcat caccgtttta aaatgactca ctattttcca cagcaaatat aaagtgtcag
    32281 tttgcgacgc gagcggcatt ttaggacata tcttccccgg ttaacagtcc ctgttgcgtc
    32341 tgtgtggcgc ttgatgccag cgggtttgcg caaaagcaaa gaaaagtgct tgttaaatcg
    32401 gcaattcatc tctgcatgta acaaaataat gcaaaaacgc gctgtcgata tcacttaatt
    32461 attgtgaatt tcagctggtg agttaattaa cgggcgggtt taaaagaact tatcaataat
    32521 agtcaggaat agtcttattt actttaagga tattgatgtc cattcccgta ctctacgcgc
    32581 cagagggaaa ttcacctggc gcgtattttt gttcgcggct tagctacggc tgagcacacc
    32641 gcgacgatag agattacgtt taatgcgcgt taaaccaggt tttggcttac gcggttcgtc
    32701 caggcttgcc aggacaatct ccagcacacg ctctgcgaca tcgcggtgac gttgagccac
    32761 tgccagcacc gggcactgta agaagtcgag cagttcgtta tcgccaaagg tggcaattgc
    32821 caggtcagaa ggcagtttac catcgcgacg tagcgttaca tccatcaccc cttgcaacaa
    32881 cgcaaacgac gttgtgaaca gcgcctgcgg catcggatgc gtttccagcc atttttcgaa
    32941 taactgggca gccgcctccc gctcatagct gttggcatac aggaaatgca cttcgcgcgg
    33001 atcatctttc caggcagtac ggaaaccttg ttcacgcagg aagctgacag aaagctccgg
    33061 cagcgcgcca agataaagca ccgtctcggc gggaaactta cgtaactctt ccgccagcat
    33121 ttcggcatca tcctgatcgg caccaaccac gctggtgaag tgttcacgat cgagggcgcg
    33181 gtccagcgcg acaatcggga acgggtcgtt agcccagcgt tgataaaaag gatgctcagg
    33241 aggcaacgac gtcgaaacaa taatggcatc gacctgacgc tgtaaaaggt gctcaataca
    33301 gcgcatttcg ttgtctggct gatcttctga gcaggcaatt agcagttgat aaccccgttg
    33361 ccgcgcctgg cgttcaagat agttagcgat gcgggtatag ctggtgttct ccagatcggg
    33421 gatcacaaga ccaatagaac gtgtgcgtcc agcacgaagc ccagctgcca cggcgttcgg
    33481 gtggtaattg tgctcacgca ccacagccat gactttttca acggttttgt cgctcacacg
    33541 gtattgcttc gctttgccgt taataacata gcttgcagtg gtccgcgaca ctcccgccag
    33601 ccgagcgatt tcatccagtt tcacaattgc cccttgcgta aaatgtaaaa accatagcca
    33661 ttgtactggt atgggttaaa ttcattaaca tctaagcgca gaaaattaac tgcggcaact
    33721 gcttttattc caggttccca ctgatttcgc aaaaaaagcc caacgtgaca tgttgggctg
    33781 caaattgcgc actgagatca ttctcaacgc attattttat cgccgcgcga aagtccgacc
    33841 acaccagagc gagcaacttc cacaattttc gccacatcgc gaatcgatgc taaaaatgca
    33901 tcaagcttat cgctggtgcc tgctaattga acggtataaa gcgagggggt gacatcgata
    33961 atttgcccac ggaatatttc cgtattacgt ttcacttcgt cacgcccgta accgctggcc
    34021 tgaattttca ccagcatgat ttcccgctca acatgcgcgc cctgccccaa ctcactcacg
    34081 cgcaagacat cgaccagctt gtgtaattgc ttttcgatct gctcaagcac tttttcatcg
    34141 cccacggtct ggatggtcat acgcgataat gtcggatcgt cggttggcgc aacggtcagg
    34201 ctttcaatgt tgtagccacg ctgggaaaaa aggccaatca cgcgggataa cgcgcctgat
    34261 tcattttcga gtaagactga taatatccgg cgcataatca ggttctctcc gttttgctta
    34321 accacatttc atccattccg cccccgcgaa tctgcatcgg gtagacgtgc tcactgccat
    34381 cgacggtaac atcaacaaac accaggcgat tattgcgcac ctgttccagc gcctcgctaa
    34441 gtttgctttc cagctcatgc ggatgagaga tctggatccc gacgtgacca taggcttccg
    34501 ccagacggac gaaatcgggt agtgattgca tataagattg tgaatgacgg ccggaataga
    34561 tcatgtcctg ccactgtttc accatcccca gatagcggtt attgagattc accaccagta
    34621 ccggcaactc gtattgcaac gcggtagaca gttcctggat gttcatctga atactgccgt
    34681 cgccagtgac gcaaaccacg gtttcttctg gcaacgccat tttgacgccc agtgccgcag
    34741 gtaaaccaaa acccatcgtg ccgaggccac cggaattgat ccagcgacgc ggtttgtcga
    34801 atggataata aagtgcagca aacatctggt gctgcccgac atcggacgtc acgtaagcgt
    34861 ctcccttcgt caaccgccaa agagtctcga tcaccgcctg cggtttaatc ttttcactgt
    34921 gagtgtcata tttcaggcac tgacgagcgc gccactgttc aatctgctgc caccagtcgc
    34981 ggatctcatc cagcggttga tgggcggatt cttgcgacag gagttcaagc atttgttcga
    35041 ggacctggcg agcatccccc acaatcggga tatcagcagt cacggtttta gaaatggaag
    35101 taggatcaat atcgatatgc agaacagtgg catttgggca gtactttgcc agattattcg
    35161 tcgttcggtc atcaaatctt accccgacgg cgaaaatcac atccgcgtta tgcatcgtca
    35221 tattggcttc gtaggtgccg tgcattccca gcatgcccag cgcctgacga tgcgttgccg
    35281 gaaatgcccc caggcccatc aatgaggaaa caacgggcag attcaacgcc tccaccgttt
    35341 ctttcaactg ctgatggcag cccgccgtga ttgccccacc gcctacgtag acaaccggtt
    35401 tttttgccgc caccagcgtt tgcagagcac gcttaatttg ccctttatgt ccggtagtag
    35461 tgggattgta agaacgcata ctaaccgact ccggccagac atagggtaat ttgttcgccg
    35521 gattaagaat atctttcggt aaatcaacga ctactggtcc aggacgacca cttgccgcca
    35581 gccagaaagc ctttttcagc acctgcggaa tgtcttccgt ttgcttaacc agaaaactgt
    35641 gtttaaccac cggtcgcgaa atccccacca tgtcgcactc ctgaaaggca tcgtaaccta
    35701 tcaacgaggt cgctacctgc ccggaaagga caactaatgg aatggaatcc atataagcgg
    35761 tggcgatgcc ggtaatcgca ttggtcgccc ctggacccga cgttaccagc acgacgccga
    35821 cttccccggt cgcacgcgcc aggccatcgg ccatatgcac cgccgcctgc tcatgacgaa
    35881 ctaatacatg atcaatacca cccacggtat gcaatgcatc gtaaatatca aggactgcgc
    35941 ctccgggata accgaatact tgtttaacgc cctgatcgat aagcgatcgg acgaccatct
    36001 cggctccaga caacatctcc atggcctgcc tcactgtttg acggaaaaaa tgtgtaaaac
    36061 acattaaccg tccgatcttg cttaagcaat tggcagccag agacataaac gcagggtaat
    36121 aagaataaac aggaggaaag gtgaaaagaa acgataaaat cctccattac gaaggattaa
    36181 attgacaata aatcggctga atcccacaac ttacagcaat ttctctattt ttttaattaa
    36241 tcctaaaata gagagcacac acattcagaa taaaattcta cattcaataa aaaatgcaga
    36301 ataaaccaga cattcatgtc tggcctattc tgcattcagt tagcgtttgc aaattgagac
    36361 taattgttct tccatccact gatggccttt atcgcgcccg gcagcttcat gccaggagag
    36421 ataacaggtt ctgctgtttt gttttaacgg cagcggtaat acctgtaatt ctaaggattc
    36481 agcgaactct tcagccagcc aacgcggcgc aatagcgacc aaatgcgttt gcgacaccac
    36541 gctaagtacg ctcatcattg ccatgccctg atacgcgata ctggcttgct tatctaccgt
    36601 gtcataccaa ggttgactaa atgacgcgaa acgttcgagc gaaaccgccg catgttgttc
    36661 gttataaaca tcatgtttca gtaacgggcc cttaattgtt ggatgatttt tgctggctac
    36721 cagcaccatt tcatctttaa ataatggcac gctggtaaat tcaggacgat ggaagtcttc
    36781 ataactaatc acaaactccg tttcctgata acgcagctga tgttcagtgt tctgatttaa
    36841 tgaagacttg aacataacat gtatatttgg cgcaatctgc tcaatgtgat tataaatctg
    36901 cgaggtcaga atgctgtcta acgggctgca aacacaaaga tgaaatacac gttcactgct
    36961 cgcgggttca aaacctgaac caggcaattc attttgtact agttgcaatg cctgacgaac
    37021 tgagccaaaa agttgaaatg cgcgagcagt cggttgaata ccacggccat aacgaacaaa
    37081 aagctcgtca ttaaacatca ccttcaggcg tgcaacagcg ttactgaccg caggttgcga
    37141 catacccaga acatgagcgg cacgggtaat gttttgctcc tgcatcacgg catcgaaaac
    37201 ggttaataag ttgagatcga ccatgcgtag ctgtggcttg cttaactccg ccgtctctga
    37261 atgatctgtt tgtacctctg gcatacttaa ctccactgtc acacttaact ccctttccct
    37321 tattggaatg cagagaaata atcctgaaga atatgattta tcatgcatat aaaataagaa
    37381 aaaggaaaat gagtaaaatt cggaaaacat aaagatacgg gttctgataa aaacagaatc
    37441 cattgccgtg
//
LOCUS       DAZGXZ010000034        37234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000034 DAZGXZ010000000
VERSION     DAZGXZ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.034"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(2..154)
                     /gene="hokA"
                     /locus_tag="ACHKJF_001840"
     CDS             complement(2..154)
                     /gene="hokA"
                     /locus_tag="ACHKJF_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP0949211.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(480..2549)
                     /gene="glyS"
                     /locus_tag="ACHKJF_001841"
     CDS             complement(480..2549)
                     /gene="glyS"
                     /locus_tag="ACHKJF_001841"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP0949212.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2559..3470)
                     /gene="glyQ"
                     /locus_tag="ACHKJF_001842"
     CDS             complement(2559..3470)
                     /gene="glyQ"
                     /locus_tag="ACHKJF_001842"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP0949213.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3565..3864)
                     /locus_tag="ACHKJF_001843"
                     /pseudo
     CDS             complement(3565..3864)
                     /locus_tag="ACHKJF_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
     gene            4039..5034
                     /gene="wecH"
                     /locus_tag="ACHKJF_001844"
     CDS             4039..5034
                     /gene="wecH"
                     /locus_tag="ACHKJF_001844"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP0949214.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5076..5513)
                     /gene="yiaA"
                     /locus_tag="ACHKJF_001845"
     CDS             complement(5076..5513)
                     /gene="yiaA"
                     /locus_tag="ACHKJF_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP0949215.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5559..5900)
                     /gene="yiaB"
                     /locus_tag="ACHKJF_001846"
     CDS             complement(5559..5900)
                     /gene="yiaB"
                     /locus_tag="ACHKJF_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP0949216.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(6069..7523)
                     /gene="xylB"
                     /locus_tag="ACHKJF_001847"
     CDS             complement(6069..7523)
                     /gene="xylB"
                     /locus_tag="ACHKJF_001847"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP0949217.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7595..8917)
                     /gene="xylA"
                     /locus_tag="ACHKJF_001848"
     CDS             complement(7595..8917)
                     /gene="xylA"
                     /locus_tag="ACHKJF_001848"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP0949218.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            9283..10275
                     /gene="xylF"
                     /locus_tag="ACHKJF_001849"
     CDS             9283..10275
                     /gene="xylF"
                     /locus_tag="ACHKJF_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0949219.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            10353..11894
                     /gene="xylG"
                     /locus_tag="ACHKJF_001850"
     CDS             10353..11894
                     /gene="xylG"
                     /locus_tag="ACHKJF_001850"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP0949220.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11872..13053
                     /gene="xylH"
                     /locus_tag="ACHKJF_001851"
     CDS             11872..13053
                     /gene="xylH"
                     /locus_tag="ACHKJF_001851"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP0949221.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIILTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            13131..14309
                     /gene="xylR"
                     /locus_tag="ACHKJF_001852"
     CDS             13131..14309
                     /gene="xylR"
                     /locus_tag="ACHKJF_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP0949222.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14417..15241)
                     /locus_tag="ACHKJF_001853"
     CDS             complement(14417..15241)
                     /locus_tag="ACHKJF_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP0949223.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15560..17590
                     /gene="malS"
                     /locus_tag="ACHKJF_001854"
     CDS             15560..17590
                     /gene="malS"
                     /locus_tag="ACHKJF_001854"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0949224.1"
                     /translation="MKLAACFLTLLPGFAVAANWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGEYTLQIDTRSGTPT
                     LMISIQNAAEPVASLVRECPKWDGLPLIVDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTPAPFNWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVNSAHLRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQDFNVLSYLSSHDT
                     RLFREGDDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGESA
                     ASVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17768..19021
                     /gene="avtA"
                     /locus_tag="ACHKJF_001855"
     CDS             17768..19021
                     /gene="avtA"
                     /locus_tag="ACHKJF_001855"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP0949225.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(19173..19646)
                     /locus_tag="ACHKJF_001856"
     CDS             complement(19173..19646)
                     /locus_tag="ACHKJF_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0949226.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFS
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19748..20596)
                     /gene="yiaJ"
                     /locus_tag="ACHKJF_001857"
     CDS             complement(19748..20596)
                     /gene="yiaJ"
                     /locus_tag="ACHKJF_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP0949227.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20797..21795
                     /gene="yiaK"
                     /locus_tag="ACHKJF_001858"
     CDS             20797..21795
                     /gene="yiaK"
                     /locus_tag="ACHKJF_001858"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP0949228.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGVSQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21807..22274
                     /locus_tag="ACHKJF_001859"
     CDS             21807..22274
                     /locus_tag="ACHKJF_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP0949229.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCIMQTASEIRKIVVKVALTALN"
     gene            22392..22865
                     /gene="yiaM"
                     /locus_tag="ACHKJF_001860"
     CDS             22392..22865
                     /gene="yiaM"
                     /locus_tag="ACHKJF_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP0949230.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22868..24145
                     /gene="yiaN"
                     /locus_tag="ACHKJF_001861"
     CDS             22868..24145
                     /gene="yiaN"
                     /locus_tag="ACHKJF_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP0949231.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            24158..25144
                     /gene="yiaO"
                     /locus_tag="ACHKJF_001862"
     CDS             24158..25144
                     /gene="yiaO"
                     /locus_tag="ACHKJF_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP0949232.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPHGADLLKAVDEVQ"
     gene            25148..26644
                     /gene="lyxK"
                     /locus_tag="ACHKJF_001863"
     CDS             25148..26644
                     /gene="lyxK"
                     /locus_tag="ACHKJF_001863"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP0949233.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAALTGLKAGTSVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKVG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHTL"
     gene            26641..27303
                     /gene="ulaD"
                     /locus_tag="ACHKJF_001864"
     CDS             26641..27303
                     /gene="ulaD"
                     /locus_tag="ACHKJF_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0949234.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     SAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHTMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQARGQQWGEADLALMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            27296..28156
                     /locus_tag="ACHKJF_001865"
     CDS             27296..28156
                     /locus_tag="ACHKJF_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP0949235.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            28150..28845
                     /gene="araD"
                     /locus_tag="ACHKJF_001866"
     CDS             28150..28845
                     /gene="araD"
                     /locus_tag="ACHKJF_001866"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0949236.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(28877..29692)
                     /locus_tag="ACHKJF_001867"
     CDS             complement(28877..29692)
                     /locus_tag="ACHKJF_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0949237.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFIVLLILQQLTRDARLDEQADAAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            29918..31318
                     /locus_tag="ACHKJF_001868"
     CDS             29918..31318
                     /locus_tag="ACHKJF_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949238.1"
                     /translation="MTSTPITTADIAKGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            31329..33293
                     /locus_tag="ACHKJF_001869"
     CDS             31329..33293
                     /locus_tag="ACHKJF_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFP0949239.1"
                     /translation="MNISEVDLRKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGA
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFTSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPADAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     TQTLTFIPWFSWANRGEGEMRIWVNEETSA"
     gene            complement(33365..33775)
                     /locus_tag="ACHKJF_001870"
     CDS             complement(33365..33775)
                     /locus_tag="ACHKJF_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0949240.1"
                     /translation="MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALV
                     AHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWLLVDINMNQFEGRAERINITMPK
                     RLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGA"
     gene            complement(33792..33976)
                     /locus_tag="ACHKJF_001871"
                     /pseudo
     CDS             complement(33792..33976)
                     /locus_tag="ACHKJF_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833473.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(34254..35792)
                     /gene="aldB"
                     /locus_tag="ACHKJF_001872"
     CDS             complement(34254..35792)
                     /gene="aldB"
                     /locus_tag="ACHKJF_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP0949241.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(35957..37108)
                     /gene="yiaY"
                     /locus_tag="ACHKJF_001873"
     CDS             complement(35957..37108)
                     /gene="yiaY"
                     /locus_tag="ACHKJF_001873"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP0949242.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
BASE COUNT         9061 a         9635 c         9381 g         9157 t
ORIGIN      
        1 cttactcttt taaattgcag gctaaaaatg ccgccagctc ataactcccc tgtttaatat
       61 gcaattcaca cagtgaatct cttatcatcc aggtgaaaaa taaaagcgtg aaacaaatca
      121 ctattaaaga aagtaatcta tatttctgcg gcattcccag ccccctgtgt tgatttccaa
      181 cgagtaagag gctaacctta tggtgttcag gcatagggta gcctcacttt gatttatagt
      241 caggtggggc ttttctctgt ctgcctttcg gtgaatacct gagacaaaca gtctcaagca
      301 cccgtggcta ttctagctta ataagtttgt ttcttctcct tgatataatc ctaaaaaaac
      361 ctcataaaat taatatatga gataatcttt attcagcaga agattattaa aggttgctgt
      421 attatttagc gataataaaa aaagcctgcc agatggcagg ctttttaata acggcgttat
      481 tactgcaaca gcgaaatatc cgcaacgcgc aggaacagtt cgcgcagttt ctccagcatg
      541 gtcagacggt tgagacgcaa ttctttgtca tcaaccatga ccatcacttt atcgaagaaa
      601 gcatcaaccg gctcacgcag ctctgccagt tcgaccagcg catcctggta acgaccttcc
      661 gcaaagtacg gctccagctt gtcacgtagc accacaacct gcatcgccag tttaatttct
      721 tccggctctt tcagggtaga ggcattcacg cggtcgctca gcacttcgtc agatttcgcc
      781 aaaatgttgg agacacgctt gttcgccgcc gccagtgcag cagctgcttc aagggtacgg
      841 aagtgcgata ccgctttcat gcgggcatcg aaatcagccg gacgagtcgg acgacgcgcc
      901 agtaccgcct ggatggtgtc aacggtgtaa ccttcgtcct gataccaggc gcggaagcga
      961 ccgagcataa agtcgataac atcatcaacc acgttggcgt tagtcagctt atcgccatac
     1021 agacgcactg cttcttcggt cagcgtttgc agatcaaggt tgaggttctt ctcaacgata
     1081 attcgcagca cgccaagtgc ggcacgacgc agcgcaaacg ggtctttgtc gcctttcgga
     1141 tgctggccga taccgaagat acccgccaga gtatccatct tgtcagcaat cgccagcgca
     1201 caggctaccg ggttagacgg caggtcatca ccggcaaagc gcggctgata ctgctcattc
     1261 agcgccaccg cgacatcttc cgcttcgcca tcgtgacgcg catagtgcat ccccataacg
     1321 ccctgggtgt cggtgaactc gaagaccatg ttggtcatca ggtcacactt agacagcagg
     1381 cccgcacggg ttgcgtggtt aacgtcagcg ccaatctgtt cagcaatcca gccagccagc
     1441 gcctggatgc ggtcagtttt gtcacgcagc gtacccagct gttgctggaa cagcacggtt
     1501 tgcaggcgcg gcaggttatc ttccagacgt tttttacggt cggtgttgaa gaagaactcg
     1561 gcatccgcca gacgcggacg aacgactttc tcgttaccgg agataatctg ctgcggatct
     1621 ttcgattcga tgttggcaac gaagataaag ttcggcagca gtttgccgtc gttcgcatac
     1681 accgggaagt atttctggtc acctttcatg gtgtaaacca gcgcttcagc cggcaccgcg
     1741 aggaatttct cttcgaattt tgcggtcaga acgaccggcc actccaccag cgaagccact
     1801 tcttccagca ggctttcgct taagtcagcg ttaccgccaa tcttacgcgc tgcttcttcg
     1861 gcatcggctt taatcttcgc tttacgttct tcgtaatcgg cgatgacttt cccgcgctca
     1921 cgcagaattt ccggatactg atcggcgttg tcgatggtga attccggttc gcccataaag
     1981 cggtggccgc gaatcacgcg atcggactga atgcccagaa tggttgccgg aatgactttg
     2041 tcgcccagca gcagggtcac ggtgtgcacc ggacgcacga agtgcacgtc gcttgcgccc
     2101 caacgcatca gtttcgggat cggcagtttc gccagagaag tcgcaaccat attcggcagc
     2161 agtgcttcgg tgctttcgcc cttcacatgg gcgcgataca gcagccattc gcctttatcg
     2221 gtagtcagac gctcagcctg gtcaacggta ataccgcaac cacgcgccca accttctgcc
     2281 gctttgctcg gtttgccttc agcgtcgaac gcctgggcaa tcgccgggcc gcgtttttcg
     2341 atttcacgat ccggttgcgc ttccgccagg ttagctactt tcagcgccag acgacgcgga
     2401 gcagcaaacc attgaacggt gccgtgtgcg aggccagcgt tatccagctc cgcagtaaaa
     2461 ttcgcagcaa aggactcagc caggctgcgc agtgcttttg gtggcagctc ttcagtgccg
     2521 atttccacca gaaaagtttt ctcagacata gccgcctctt acttatcttt gttgcacatc
     2581 gggaagccga gggcttcacg ggaagcgtag tatgcttctg ccactgcttt ggtcagggtg
     2641 cgaatgcgca gaatgtagcg ctgacgctcg gtgacggaga tggctttacg cgcatccagc
     2701 aggttgaagc tgtgggcggc tttcagaata cgctcgtagg ctggcagcgg cagcggattt
     2761 tccagcgcca gcagctgctg cgcttctttc tcgtactgct caaagcaggt gaacaggaag
     2821 tccacatccg cgtattcgaa gttgtaagtg gactgctcca cttcgttctg atggaacacg
     2881 tcgccgtagg tggttttacc cagcgggccg tcgctccaga ccaggtcgta aacgctgtct
     2941 acgccctgaa tgtacatggc cagacgttcc agaccgtagg tgatctcgcc ggtaaccggt
     3001 ttacactcca gaccaccaac ctgctggaag taagtgaact gcgtcacttc catgccgttt
     3061 agccacactt cccagcccag tccccaggca cccagagtcg ggttttccca gttatcttcc
     3121 acgaaacgga tgtcgtgaat agtcgggtcc atgcccagct ctttcagaga accgaggtac
     3181 agctcctgaa tattgtccgg cgatggctta atgaccacct ggaactgata gtagtgctgt
     3241 aaacggttgg ggttttcgcc gtagcgacca tcggtcgggc gacgagaagg ctgaacataa
     3301 gcagccgcca tcggttctgg ccccagcgcg cgcagacagg tcattgggtg agaggttccc
     3361 gcgccgactt ccatgtccaa tggttgaaca atggtgcagc cctggcgagc ccagtaatcc
     3421 tgtaaggtca ggatcaagcc ctggaaggtc ctggtatcaa acttttgcat attatttcgt
     3481 gctggatacg tgtggattta aaggaaggga tcagtatacc cgctggatgg aagatataca
     3541 gtacgaaacg ggaaaaagca gggcttaacg catggaaaga tgcaaaaaat ggccatctgc
     3601 gtcaaaagaa cagacaaact gctctttacg gaaggtataa ccgcgcattt catagcttcc
     3661 ctagaactgt tcgaaggcgg tgacgtcgat tttttgctcg ccggtgttat aacgttttgc
     3721 cgcctgatcc ttgcataacg cttccatatt caacgaacgc agaggatcaa cctttacccg
     3781 ttgtgctttc tgcacgggtg acggtgtaga acaacccact agcaccataa gcgccattgc
     3841 cggaaagaaa gcgttcatca tcattaaatt accgtctggt ttgcaggtga gtcttattat
     3901 ttatatcggt atagtggctg atatcttacg aatttcctta agcctctata agctgtagtg
     3961 ataaaactca gattattcca gtgcgccaac tgaatgattt atcggtcaga ctttttttaa
     4021 ccagatacag agacacagat gcagcccaaa atttactgga ttgataacct gcgagggata
     4081 gcgtgtttaa tggtggtgat gattcacacc actacctggt atgtgaccaa tgctcatagt
     4141 gttagccccg tcacctggga tatcgccaat gttctgaatt ctgcctctcg tgtcagcgtg
     4201 ccgctatttt tcatgatttc cggctatctc ttttttggcg aacgcagcgc ccagccgcgc
     4261 catttcttgc gtatcggctt atgtctgatt ttttatagcg caatcgcact gctctacatt
     4321 gcgctgttta cctccatcaa tatggagtta gcgctgaaaa acctgctgca aaagccagtg
     4381 ttttaccact tgtggttttt cttcgcgatt gcggtgattt atctggtttc accgctgatt
     4441 caggtgaaga acgtcggcgg aaaaatgttg ctggtgctaa tggtggtaat tggcattatc
     4501 gctaacccaa acacagtgcc gcagaaaatt gacggttttg aatggctgcc aattaactta
     4561 tatatcaatg gcgatacttt ttactacatt ctgtatggca tgttgggccg cgctataggg
     4621 atgatggaca cacagcataa agcactgtcg tgggtgagcg ccgcgctgtt tgcgacgggg
     4681 gtttttatta tctctcgcgg gacattatat gaattgcagt ggcgcggaaa ttttgccgat
     4741 acctggtatc tttactgtgg gccgatggtt tttatctgcg caatcgcgct attgactctg
     4801 gttaaaaaca cgctggatac gcgtaccatt cgcggacttg gcttaatctc ccgccattca
     4861 ttgggtatat acggattcca cgccttgatt atccatgcgc tgcgcacccg gggaattgag
     4921 cttaaaaatt ggccaatact ggatattatt tggatctttt gcgcgacgtt ggcagcgagt
     4981 ttgttacttt ctatgctggt acaacgaatc gacagaaaca gattagtgag ttaagtaaaa
     5041 gcccgttcac attggactga ccgggcttac gtgaattatt cgctgtattc ttcctgggta
     5101 acctgtgttt cttttggcgg gtttattccg gtatcacgaa tattcttctg caccgctact
     5161 gcaccaaaca agcttaagaa gaaagccagt ccataaaaac ctttttcact gagtaataat
     5221 gtcgcattcc acagacctac catcagtaat gcaacagaga taataaagac agtcaggcag
     5281 gtcatataat aaatggaagt ggtcggtatg ccttcatact tgtcccgaac ggtcttttga
     5341 taagacgcag cagaaaacag tcctaacacc agtacggcaa aataataccc tttttcattt
     5401 aactgcatct ctgcattcca tagccctaac agataggtaa cgataccacc aacgagagct
     5461 atccatgaca caatactaaa ggccggtgaa taggttgata ttttgttgtc catcacagta
     5521 ttcctttcat tcctgaatgt gtaagagctt tatgttgctc ataccgagcg aaacaaacgc
     5581 atttgaccaa acaagcagac aaaaaaggcg atggggtaga tcaccatttc ataagcattg
     5641 ataggtgcat ttaaaacacc gataaacaag agaccgatag tgattaacgc aactaactgg
     5701 cagatatggg taaaaaaatt atccagttgc ccgagttttt ctgcgcgaag atatgcatag
     5761 ttaccaaaag ttgctgtcat taacacgcca agaaaatagc cttttccact tagcaatggg
     5821 catgagatcc atagcccaac cagataaacc agcgccccga caacaaataa tagttttgcc
     5881 acagtttttg aggttttcat aacgatctcc atatctacca gcgatacatt acgagtaacc
     5941 aacgaaagac aaaactgaaa aatgccatta acaaatgatt ttcagaataa attcatacta
     6001 aatattaatt aattagtgag atatatagat gtgaattatc ccccacccgg tcaggcaggg
     6061 gataacgttt acgccattaa tggcagaagt tgctgataga ggcgacggaa cgtttcacgt
     6121 cgtggctgat aagcggcata acgctgcgca tctggtagat gcgactgttc taacggtagc
     6181 tgcggcaaca actcaatgag cgatttctct ggattcgccg caatctgcgc cagccttgct
     6241 gcgcccagtg ctggccccac atcccccccc gtacggtaat cgagctgctg accgctgata
     6301 tccgccagca tctgacgcca gtactcacta cgcgccccgc caccaatcaa cgtaacactt
     6361 tgcggtttaa tgccgcaggc atgcacgaca tccatgccat ctgccagcgc ataacccacg
     6421 ccttccagca ctgctcgcgc cagttcattg gggccatgtt gatgagtcaa accaaagaaa
     6481 acccccttcg cctggggatt attgtgtggt gtacgctcgc cggaaagata aggcagaaac
     6541 caaactggct cggcactttc atcagcctgt tgagctgcag cgattaaagc tgggacattg
     6601 cacaggccgg ttaatttcgc gacccaatcc agacacgatg ctgcactcag cattacagac
     6661 attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt gtacagcgct ttctggcttg
     6721 cttaagaacc cttcgctgac agcaaaatag acccccgacg tccccagcga taacattgcc
     6781 tgattagcat caaccattcc cacaccaact gcaccagctg cattgtcgcc accgcctgcg
     6841 acaactggca ccgtcgccat accccacgct ttcgcaactt caggtaacaa agcaccagta
     6901 atttcgctgc cttcatataa tgcgggcatc tggtcacgag ataagtcgca agcctgcagc
     6961 atgacgtcac tccagtcacg ctttgcgaca tccagccaca tggtgccagc tgcgtcagac
     7021 atatcgctgg caaactcccc cgtcatacgc agacgcaagt aatctttcgg taataatact
     7081 ttgtcgattt gacggaatat ctccggctca tgccgctgaa cccatagcaa tttaggcgca
     7141 gtaaatccgg gcatcatcag gttgccggta atcactcgtg attgcggaac tctcgcttcc
     7201 agcaaagtgc actcttgcgc acagcgcccg tcgttccaca aaatggcagg gcgcaatacc
     7261 cgttgttgag catccagtaa ggttgctcca tgcatctggc cggcaatacc caatgcttta
     7321 acgtcctgca gagaatgctg atcgcccaga gctttcattg cgcgatcagt tgcctgccac
     7381 cactgttccg ggtcttgttc cgaccagagt ggatgcgggc gcgaaacggt cagcttttcc
     7441 gtttgcgaag caaccacctc accctgctcg ttgagcaaaa taacttttac gcccgaggtg
     7501 ccaagatcta tcccgatata catatcgatc gttccttaaa aaaatgcccg gtatcgctac
     7561 cgataaccgg gccaacggac tgcacagtta gccgttattt gtcgaacaga taatgattta
     7621 ccagattttc cagttgctcc tggcgaccac tctgatgcac cggagacaaa ttatgttcct
     7681 gagcatattt ggctaaatct gccagtgaca tttggccttt caggatttgc tggcccaatt
     7741 cgctattcca gccggaataa cgctgcgcga tgcgtttatc cagctcgcca tcttcaatca
     7801 tgcgcgctgc aattttcagc gccagtgcca tcgtatccat cgcgccgata tgaccgtaaa
     7861 acagatcata tttatcagta ctttgacgac gtactttggc atcgaagttc agaccaccgg
     7921 tggtgaaacc gcctgctttg agaatttcat acatcaccag cgcattctct tccacactgt
     7981 tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc acggttggcg tcgacagaac
     8041 cgaacaggcc aagcgcaatg gcggtggcaa tttcatgatg gaaagagtga cctgccagcg
     8101 tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc cagaccaaac tgtttcagga
     8161 agccatagac cgtcgcggca tcgtaatcat attgatgttt agtcggttct tgcggtttcg
     8221 gttcgataag caacgtgccc tggaagccga ttttatgttt atgctcaacc accatctgca
     8281 taaagcggcc cagttgttca cgctcctgac gcaagtcggt atttaacagc gtttcgtaac
     8341 cttcacgacc accccacagg acatagtttt caccgcccaa tttatgggtt gcttccatcg
     8401 ctgtaacaac ttgcgttgcc gcccagctga agacttcagg atctgggttc gtcgccgcac
     8461 ccgcgccgta gcgagggttt gtaaagcagt tggcggttcc ccacagcagc ttcacgccgc
     8521 tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc aaaattattg atgtactctt
     8581 ttaacgacgc gccctcagga gaaacatcca catcgtggaa gcaataaaat ggcacatgta
     8641 acttgtggaa aaactcaaat gcgacatctg ctttacgctt cgccaacgcc agtgcctcac
     8701 caggctgctg ccacggacga ttaaacgccc ccacaccaaa catgtccgcc ccgttccagc
     8761 agaaggtgtg ccagtagcag gcggcaaatc gcaagtgctc ttccatacgc ttacccaaca
     8821 ccagttcgtc gggattgtag tgacggaatg ctaacgggtt tgaggatttt gagccttcat
     8881 aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt gaactccata atcaggtaat
     8941 gccgcgggtg atggatgatg tcgtaatatt gggcactccc tttcagttgc tcaattatgt
     9001 tatttcacac tgctattgag ataattcaca agtgtgcgct cgctcgcaaa ataaaatgga
     9061 atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat aagtacaatt gcgcaacaaa
     9121 agtaagatct cggtcataaa tcaagaaata aaccaaaaat cgtaatcgaa agataaaaat
     9181 ctgtaattgt tttcccctgt ttagttgcta aaaattggtt acgtttatcg cggtgattgt
     9241 tacttattaa agctgtcctc taactacaga aggccctaca ccatgaaaat aaagaacatt
     9301 ctactcaccc tttgcacctc actcctgctt accaacgttg ctgcacacgc caaagaagtc
     9361 aaaataggta tggcgattga tgatctccgt cttgaacgct ggcaaaaaga tcgagatatc
     9421 tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg tacagtctgc aaatggcaat
     9481 gaagaaacac aaatgtcgca gattgaaaac atgattaacc ggggtgtcga tgttcttgtc
     9541 attattccgt ataacggtca ggtattaagt aacgttgtaa aagaagccaa acaagaaggc
     9601 attaaagtat tagcttacga ccgtatgatt aacgatgcgg atatcgattt ttatatttct
     9661 ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc tggtcgatat tgttccgcaa
     9721 ggtaattact tcctgatggg cggctcgccg gtagataaca acgccaagct gttccgcgcc
     9781 ggacaaatga aagtgttaaa accttacgtt gattccggaa aaattaaagt cgttggtgac
     9841 caatgggttg atggctggtt accggaaaac gcattgaaaa ttatggaaaa cgcgctaacc
     9901 gccaataata acaaaattga tgctgtagtt gcctcaaacg atgccaccgc aggtggggca
     9961 attcaggcat taagcgcgca aggtttatca gggaaagtag caatttctgg ccaggatgcg
    10021 gatctcgcag gtattaagcg tattgctgcc ggtacgcaaa ctatgacggt gtataaacct
    10081 attacgttgt tggcaaatac tgccgcagaa attgccgttg aattgggcaa tggtcaggag
    10141 ccaaaagcag atacctcact gaataatggc ctgaaagatg tcccctcccg cctcctgaca
    10201 ccgatcgatg tgaataaaaa caacattaaa gatacggtaa ttaaagacgg attccacaaa
    10261 gagagcgagc tgtaagcgtt acgccccggc gcggagcggg ggcgtgattt ctctccatgc
    10321 cgcgtgaatt aattggctta ggtggagtcg ttatgcctta tctacttgaa atgaagaaca
    10381 ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt cagcttgcgg ttgaatgctg
    10441 gcgaaatcgt ctcactctgt ggggaaaatg ggtctggtaa atcaacgctg atgaaagtgc
    10501 tgtgtggtat ttatccccat ggctcctacg aaggcgaaat tatttttgcg ggagaagaga
    10561 ttcaggcgag tcacatccgc gataccgaac gcaaaggtat cgccatcatt caccaggaat
    10621 tggccctggt gaaagaattg accgtactgg aaaatatctt cctgggtaac gaaataaccc
    10681 acaatggtat tatggattat gacctgatga cgctacgctg tcagaagctg ctcgcacagg
    10741 tcagtttatc catttcacca gatacccgcg tgggagattt agggcttggg caacaacaac
    10801 tggttgaaat agccaaggca cttaataaac aggtgcgctt gttaattctc gatgaaccga
    10861 cagcctcatt aactgagcag gaaacgtcgg ttttactgga tattattcgc gatctacaac
    10921 agcacggtat cgcctgtatt tatatttcgc acaaactcaa cgaagtcaaa gcgatttccg
    10981 atacgatttg cgttattcgc gacggtcagc acattggtac gcgtgatgct gccggaatga
    11041 gtgaagacga tattatcacc atgatggtcg ggcgagagtt aaccgcgctt taccctaatg
    11101 aaccacatac caccggagat gaaatattac gtattgaaca tctgacggca tggcatccgg
    11161 ttaatcgtca tattaaaaga gttaatgatg tctcgttttc cctgaaacgt ggcgaaatac
    11221 tgggtattgc cggactcgtt ggtgccggac gtaccgagac cattcagtgc ctgtttggcg
    11281 tgtggcccgg acaatgggaa ggaaaaattt atattgatgg caaacaggta gatattcgta
    11341 actgtcagca agccatcgcc caggggattg cgatggtacc cgaagacaga aagcgcgacg
    11401 gcatcgttcc ggtaatggcg gttggtaaaa atattaccct cgccgcactc aataaattta
    11461 ccggtggcat tagccagctt gatgacgcgg cagagcaaaa atgtattctg gaatcaattc
    11521 agcaactcaa agttaaaacg tcgtcccccg accttgctat tggacgtttg agcggcggca
    11581 atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa cccgcgcatc ctcattcttg
    11641 atgaacctac caggggtatc gatattggcg cgaaatacga gatctacaaa ttaattaacc
    11701 aactcgtcca gcagggtatt gccgttattg tcatctcttc cgaattacct gaagtgctcg
    11761 gccttagcga tcgggtactg gtgatgcatg aagggaaact aaaagccaac ctgataaatc
    11821 ataacctgac tcaggagcag gtgatggaag ccgcattgag gagcgaacat catgtcgaaa
    11881 agcaatccgt ctgaagtgaa attggccgta ccgacatccg gtggtttttc tgggctgaag
    11941 tcactgaatt tgcaggtctt cgtgatgatt gcagctatca tcgcaatcat gctgttcttt
    12001 acctggacca ccgatggtgc ctacttaagc gcccgtaacg tctccaacct gttacgccag
    12061 accgcgatta ccggcatcct cgcggtagga atggtgttcg tcataatttc tgctgaaatc
    12121 gacctttccg tcggctcaat gatggggcta ttaggtggcg tcgcggcgat ttgtgacgtc
    12181 tggttaggct ggcctttgcc acttaccatc attctgacgt tggttctggg actgcttctc
    12241 ggtgcgtgga acggatggtg ggtcgcgtac cgtaaagtcc cttcatttat tgtcaccctc
    12301 gcgggcatgt tggcatttcg cggcatactc attggcatca ccaacggcac gactgtatcc
    12361 cccaccagcg ccgcgatgtc acaaattgga caaagctatc tccccgccag taccggcttc
    12421 atcattggcg cgcttggctt aatggctttt gttggttggc aatggcgcgg aagaatgcgc
    12481 cgtcaggctt tgggtttaca gtctccggcc tctaccgcag tagtcggtcg ccaggcttta
    12541 accgctatca tcgtattagg cgcaatctgg ctgttgaatg attaccgtgg cgttcccact
    12601 cctgttctgc tgctgacgtt gctgttactc ggcggaatgt ttatggcaac gcggacggca
    12661 tttggacgac gcatttatgc catcggcggc aatctggaag cagcacgtct ctccgggatt
    12721 aacgttgaac gcaccaaact tgccgtgttc gcgattaacg gattaatggt agccatcgcc
    12781 ggattaatcc tcagttctcg acttggcgct ggttcacctt ctgcggggaa tatcgccgag
    12841 ctggacgcaa ttgcagcatg cgtgattggc ggtaccagcc tggctggcgg tgtcgggagc
    12901 gtggctggag cggtaatggg ggcatttatc atggcttcac tggataacgg catgagtatg
    12961 atggatgtac cgaccttctg gcagtatatc gttaaaggtg cgattctgtt gctggcagta
    13021 tggatggact ccgcaaccaa acgccgttct tgattttgat aaaaattttc tcaaagccgg
    13081 ttacgtatta ccggttttga gtttttgcat gattcagcag gaaaagaacc atgtttacta
    13141 aacgtcaccg catcacatta ctgttcaatg ccaataaagc ctatgaccgg caggtagtag
    13201 aaggcgtagg ggaatattta caggcgtcac aatcggaatg ggatattttc attgaagaag
    13261 atttccgcgc ccgcattgat aaaatcaagg actggttagg agatggcgtc attgccgact
    13321 tcgacgacaa acaaatcgag caagcgctgg ctgatgtcga cgtccccatt gttggggttg
    13381 gcggctcgta tcaccttgcc gaaagttacc cacccgttca ttacattgcc accgataact
    13441 atgcgctggt tgaaagcgca tttttgcatt taaaagagaa aggcgttaac cgctttgctt
    13501 tttatggtct tccggaatca agcggtaaac gttgggccac tgaacgcgaa tatgcatttc
    13561 gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta tcaggggtta gaaaccgcgc
    13621 cagagaactg gcaacatgcg caaaatcggc tggcagactg gctacaaacg ctgccaccgc
    13681 aaaccgggat tattgccgtt actgacgccc gggcgcggca tattctgcaa gtatgtgaac
    13741 atctacatat tcccgtaccg gaaaaattat gcgtgattgg catcgataac gaagaactga
    13801 cccgctatct gtcgcgtgtt gccctttctt cggtcgctca gggcgcgcgg caaatgggct
    13861 atcaggcggc aaaactgttg catcgattat tagataaaga agaaatgccg ctacagcgaa
    13921 ttttggtccc accagttcgc gtcattgaac ggcgctcaac agattatcgc tcgctgaccg
    13981 atcccgccgt tattcaggcc atgcattaca ttcgtaatca cgcctgtaaa gggattaaag
    14041 tggatcaggt actggatgcg gtcgggatct cgcgctccaa tcttgagaag cgttttaaag
    14101 aagaggtggg tgaaactatc catgccatga ttcatgccga gaagctggag aaagcgcgca
    14161 gtctgctgat ttcaaccacc ttgtcgatca atgagatatc gcaaatgtgc ggttatccat
    14221 cgctgcaata tttctactct gtttttaaaa aagcatatga cacgacgcca aaagagtatc
    14281 gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga attgtcggcg tcgcatcagg
    14341 taactcagca caaccacctg atgcgatcaa aagccttatc cgaccgacaa gtcggataag
    14401 gctcgaggaa atacaattac atatgcgccg cgattaaccg ttggttatcc tggtacattg
    14461 cgaacaggta gttgttataa ctcttccctt tggtcgagta gcccttcagc ttgtgaatca
    14521 tcgctgtggc agtcacttcc tgatccgctt tacgcagctg cgcacgcgat ttacggaacg
    14581 aagagtaagc cggatgcgta ttgaggttag tgacataggc gctcaccgat tctttgacag
    14641 aactaaactg tgagtaccct ttcactttac ccggcgcatt ggtacaacgt cctttcatgc
    14701 atttcatgcc gaaaagattg ttgttgttgc gcgccagctt cgacgttccc caaccgcttt
    14761 ctgctgcagc catcgtcgcc accatactgg tagggataat gtctacgcgt tcaagcaagg
    14821 tattccacgg gatttttcgc gtattaccag accacttcac cttgtagcgt ttggcgatgt
    14881 ctttcagacg cgcacgctca gcaggtgacc attggccctg atactgtttt gaaatcagcc
    14941 agttacgttc cgcagtaatg gccgcatttt ggctggtaat gtaaggcatt acagtccgga
    15001 gaaacgcctt tttccttggt gttccggaag ggtattttcg caaatcagga agtgaactac
    15061 tctttgcact attgcgagaa tactcttgtt tactgcttac ctgtttatta ctagctttag
    15121 ttaagtggga cttttgactc gctgttgttg tgtgcgtctt cgctaacacc tcactcgaaa
    15181 acaccagagt gagtaacata agaatcatcg ccccatatcg tcgtatggga gtcaaaatca
    15241 tcaggtctcc tggtcggatt taatcattcc aacaccttat atttttcaca aatttgagag
    15301 ttgaatctca aatcatatca aaaatagctg tcaagagcac cccaaggaat agtccaaatc
    15361 tgaaactatg tcacgagtta acgattcaga ttggcgctaa atcgcagaaa atgtggggtt
    15421 tatcgcaaaa ttcagccgtt ttttgcgcgc gatcgctcac ccttgcttct catcctgtgg
    15481 acttaccctc agggatgagt tttgtctggc ttatcgctgg caaactgtct gaaatcgcag
    15541 caataaggac tcatccgcca tgaaactcgc cgcctgtttt ctgacactcc ttcctggctt
    15601 cgccgttgcc gccaactgga cttctccggg gtttcccgcc tttagcgaac aggggacagg
    15661 aacatttgtc agccacgcgc agttgcccaa aggtacgcgt ccactaacgc taaattttga
    15721 ccaacagtgc tggcagcctg cagatgcgat aaaactcaat cagatgcttt ccctgcaacc
    15781 ttgtagcaac acgccgcctc aatggcgatt gttcagggac ggcgaatata cgctgcaaat
    15841 agacacccgc tccggtacgc caacattgat gatttccatc cagaacgccg ccgaaccggt
    15901 agcaagcctg gtccgtgaat gcccgaaatg ggatggatta ccgctcatag tggatgtcag
    15961 cgccactttc ccggaaggag ccgccgtacg ggattattac agccagcaaa ttgcgatagt
    16021 gaagaacggt caaataacct tacaacccgc tgccaccagc aacggtttac tcctgctgga
    16081 acgggccgaa actgacaccc ctgccccttt caactggcat aacgccacgg tttactttgt
    16141 actgacagac cgtttcgaaa acggcgatcc cagtaatgac cagagttacg gacgtcataa
    16201 agacggtatg gcggaaattg gcacttttca cggcggcgat ttacgcggcc tgaccaacaa
    16261 actggattac ctccagcagt tgggcgttaa tgctttatgg ataagcgccc catttgagca
    16321 aattcacggc tgggtcggcg gcggtacaaa aggcgatttc ccgcattatg cctaccacgg
    16381 ttattacaca caggactgga cgaatcttga tgccaatatg ggcaacgaag ccgatctacg
    16441 gacgctggtt aatagcgcac atctgcgcgg tattcgtatt ctctttgatg tcgtgatgaa
    16501 ccacaccggc tatgccacgc tggcggatat gcaggagtat cagtttggcg cgttatatct
    16561 ttctggtgac gaagtgaaaa aaacgctggg tgaacgctgg agcgactgga aacctgccgc
    16621 cgggcaaacc tggcatagct ttaacgatta cattaatttc agcgacaaaa caggctggga
    16681 taaatggtgg ggaaaaaact ggatcagaac ggatatcggc gattacgaca atcctggatt
    16741 tgacgatctc actatgtcgc tggccttttt gccggatatc aaaaccgaat caactaccgc
    16801 ttctggtctg ccggtgttct ataaaaacaa aacggataca cacgctaaag tcatcgaagg
    16861 ctttacacct cgcgattact taacccactg gttaagtcag tgggtccgcg actatgggat
    16921 tgatggtttt cgggtcgata ccgccaaaca tgttgagttg cccgcctggc agcaactgaa
    16981 aaccgaagcc agcgccgcgc ttcgcgaatg gaaaaaagct aaccccgaca aagcattaga
    17041 tgacaaacct ttctggatga ccggtgaagc ctggggccac ggcgtgatgc aaagtgacta
    17101 ctatcgccac ggcttcgatg cgatgatcaa tttcgattat caggagcagg cggcgaaagc
    17161 agtcgattgt ctggcgcaga tggatacgac ctggcagcaa atggcggaga aattgcagga
    17221 tttcaacgtg ttgagctacc tatcgtcgca tgatacccgc ctgttccgtg aaggggacga
    17281 caaagcagca gagttattat tattagcgcc aggcgcggta caaatctttt atggcgatga
    17341 atcctcgcgt ccgttcggcc ctaccggttc tgatccgctg caaggtacac gttcggatat
    17401 gaactggcag gatgttagcg gtgaatctgc cgccagcgtc gcacactggc agaaaatcag
    17461 ccagttccgc gcccgccatc ccgcaattgg cgcgggcaaa caaacgacac tttcgctgaa
    17521 gcagggctac ggctttgttc gtgagcatgg cgacgataaa gtgctggtca tctgggctgg
    17581 gcaacagtga cttttccggc ttcccgttcg tcagtacctc gggaagccgc caaccaggat
    17641 aaaatgtcag ccctaatcag agttgcagga taaagcacca ctcacccttc aacagaccga
    17701 tttgcacccc agcaaatgta gcgttattgt taccttcctt gctacagagt tcgacagata
    17761 tcccgctatg acattctccc tttttggtga caaatttacc cgccactccg gcattacgct
    17821 gctgatggaa gatctgaacg acggtttacg cacgcctggc gcgattatgc tcggcggcgg
    17881 taatccggcg cagatcccgg aaatgcagga ctacttccag acgctactga ccgacatgct
    17941 ggaaagtggc aaagcgactg atgcactgtg taactacgac ggtccacagg ggaaaacgga
    18001 gctactcaca ctgcttgccg gaatgctgcg cgagaagttg ggttgggata tcgaaccaca
    18061 gaatattgca ctaacaaacg gcagccagag cgcgtttttc tacttattta acctgtttgc
    18121 cggacgccgt gccgatggtc gggtcaaaaa agtgctgttt ccgctggcac cggaatacat
    18181 tggctacgct gacgccggac tggaagaaga tctgtttgtc tctgcgcgtc cgaatattga
    18241 actgctgccg gaaggccagt tcaaatatca cgtcgatttt gagcatctgc atattggcga
    18301 agaaaccggg atgatttgcg tctcccggcc gacgaatcca acaggcaatg tgattactga
    18361 cgaagagtta ctgaagcttg acgcgctggc gaatcagcac ggtattccac tggtgattga
    18421 taacgcttat ggcgtcccgt tcccgggtat catcttcagt gaagcgcgcc cgctgtggaa
    18481 tccgaatatc gtgctgtgca tgagtctttc caagctgggt ctacctggct cccgctgcgg
    18541 cattatcatc gccaatgaaa aaatcatcac cgccatcacc aatatgaacg gcattatcag
    18601 cctggcacct ggcggtattg gtccggcgat gatgtgtgaa atgattaagc gtaacgatct
    18661 gctgcgcctg tctgaaacag tcatcaaacc gttttactac cagcgtgttc aggaaaccat
    18721 cgccatcatt cgccgctatt taccggaaga tcgctgcctg attcataaac cggaaggagc
    18781 cattttcctc tggctatggt ttaaggattt gcccattacg accgagcaac tctaccagcg
    18841 cctgaaagca cgcggcgtgc tgatggtgcc ggggcacaac ttcttcccgg ggctggataa
    18901 accgtggccg catacgcatc aatgtatgcg catgaactac gtaccagagc cggagaaaat
    18961 tgaggcgggg gtgaagattc tggcggaaga gatagaaaga gcctgggctg aaagtcacta
    19021 aaaattgcct gatgcgctac gcttatcagg cctacatgat ttctgcaata tattgaattt
    19081 ggaagaattt gtaggccgga taaggcgttt acgccgcatc tggcataaac aaagcgcact
    19141 attccggcgt tgagaaacgc cggaaaaacg tttcaaacag gctgctgccg tagccgatgc
    19201 cgctgcactt tctcgacatc catgcactgc aacgcctttg tcgggcaggc ttcaacacag
    19261 gccggtcccg tctcccgatg ccagcacaga tcgcacttaa tcgccctcgc ctttttgcgc
    19321 gaagaaacga cctccatcgc actaaacggg caagccaaca tacagctttt acagccaatg
    19381 caacgtgttt gttcaacgaa aatatgccca tgttcgcggc ttatcgcgtc aacagggcag
    19441 acattcgcgc acggtgcatc ttcacactga tgacaggcta ctgccgtggt ccagctgtgg
    19501 tctttaatga cacgaatacg ggaaataaac tcgtctggtg acaacgcagc gcaatcctga
    19561 ttctcctgat gcgacactgc gcaagccact tcacaggtac ggcaaccgat acatttcgtc
    19621 gcatccgcaa taataaaccg gttcatgatc actcctttgc actaaaacag cagagtgcca
    19681 gagagcgcgt ccctactgct ttgatccaac gaaggaaatg ttaaagtttt ggcacttgtc
    19741 caaagcgtta tgtgatcgcg cccaggtcat ccctgacagt aaatcccagt tcattagaaa
    19801 tagcctgcgc ggtttcacgc agtggtttca ggagattttt ctcccccacc tgtttcagac
    19861 gtgatgtcga aagcgaaatc gacacggcgt acggcacccg cccatgaata tcaaacaccg
    19921 gaacagcaat acaggagacg ccgagttcgt tttcttctct gtccatcgcc gctccgcttt
    19981 cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc ggtaatggta ttgcgggtta
    20041 acggctggat ctcatgctga tggctttccc agtatgactt cacgtagtcc gggtgaccaa
    20101 acgccatgta gatcttgccc attgcggaac agtagagcgg catatgctgg ccaatatagg
    20161 cacgggttcg cagcatcccg gttgtgggtt ccagcttata aatcaaaata gcgtgatcgt
    20221 cttcgcggct ggagaagtta atggtttcac cagtggcgat gttcagtgcc tcaagatgcg
    20281 gagcggcaat atgaatgata ttcagcgatg acagcgcctt ctgcccgacg gcaataaatt
    20341 tggtagtcag gcggtaactc cctgcggcgg gcgcggtggt cacatagccg caggactgta
    20401 atccctgcaa taagcgatgg acggtactct tatttaaacc agccagctcc gaaagatgcg
    20461 ccaacggaca accgtttgga tagttgctca aaatctcaat cagcatcaac ccgcgaaaca
    20521 gactctggct tccggctgga cgctcttttt cctgcgccat ctcgttctct ttttttccca
    20581 tcacttcttt ccccattttg tcgcgtcctg atggtagcgc aaagtgtgcc gtagttcacg
    20641 atctcgacag ataatttata accacttgat ttttaagttt tttgaaattc atcaatcaga
    20701 ttgctttgtt aaaaagcgat cgatatattt gaaatcaagt ttcgcatatt gaaattttaa
    20761 gccaaaaaag cgatcaaaaa aacaaggaag cctgggatga aagtgacatt tgagcagtta
    20821 aaagcagcct ttaatcgggt cttaatttca cgcggcgttg acagcgaaac ggctgacgcc
    20881 tgtgcagaga tgttcgcccg caccaccgaa tccggcgttt attctcacgg cgttaatcgt
    20941 ttccctcgtt tcattcaaca gctggaaaac ggcgatatca ttcctgatgc ccaacccaaa
    21001 cgtataacca gcctcggcgc aattgaacag tgggacgccc agcgttcgat cggcaatctg
    21061 acggcgaaaa agatgatgga tcgcgccatt gaactggctg ccgatcacgg tattggtctg
    21121 gtggcactac gtaatgccaa ccactggatg cgcggtggca gctacggctg gcaggcggcg
    21181 gaaaaaggct atattggcat ttgctggacc aactccatcg ccgtaatgcc gccgtggggc
    21241 gcaaaagagt gtcgcatcgg cactaacccg ctgatcgtcg ccattccttc cacgccgatc
    21301 accatggtcg atatgtcgat gtcgatgttt tcctacggca tgttagaagt taaccgcctg
    21361 gcgggccgtc agctcccggt cgatggtggc tttgatgatg agggcaattt gaccaaagaa
    21421 cctggcgtta tcgagaagaa tcgccgcatt ttgccgatgg gctactggaa aggttctggc
    21481 atgtcgattg tgctggatat gatcgctact ctcctttccg acggcgcatc ggttgccgaa
    21541 gtcacccagg acaacagcga cgaatacggc gtttcgcaaa tctttatcgc catcgaagtg
    21601 gataaattga tcgacggccc cacccgcgat gccaagctgc aacgcatcat ggattacgtt
    21661 actaccgctg aacgcgctga cgaaaaccag gccatccgct tacccggcca tgaatttact
    21721 accctgctgg ccgaaaaccg ccgtaacggc atcactgttg atgacagcgt gtgggccaaa
    21781 atccaggcgt tataaggaga taagccatga tttttggaca tatcgcgcag ccaaacccgt
    21841 gccgtttgcc cgccgctatt gaaaaggcgc tcgattttct gcgcgctacc gatttcaacg
    21901 ccctggagcc gggcgttgtc gaaatcgacg gcaaaaatat ttatgcgcaa attatcgatt
    21961 taaccacacg cgaagcggtg gaaaaccgtc cggaagtgca tcgtcggtat atcgatattc
    22021 agtttctggc gtggggtgaa gagaaaatcg gcattgctat tgatacggga aataataaag
    22081 tcagcgaatc actattagag cagcgcgata ttatttttta tcatgacagt gaacatgaat
    22141 catttataga aatgataccg ggcagctacg caattttctt tccgcaggat gttcaccgac
    22201 caggttgtat tatgcaaaca gcctctgaga tacgaaaaat tgtggttaaa gtcgcattaa
    22261 cggcgctgaa ttaatcgaat aatcgtctac gccagaacgc ctgggtaatg ttattgctct
    22321 aactatatta ataattcagg ttgctgcaca tgcaacttga attatgacag acatagcctc
    22381 aaggaatagc tatgaaaaaa atactcgaag caatactggc gattaatctc gccgtacttt
    22441 cctgtattgt atttataaac attattttaa gatatggttt tcagacaagc attttatctg
    22501 ttgatgaatt gtcacgttat ttatttgtct ggttaacatt tattggcgcg attgtggctt
    22561 ttatggataa cgcccacgtt caggtcacct ttctggtgga gaaactctcc cccgcatggc
    22621 aacgacgagt cgctctggtt acccattcct taatcttatt tatttgtggc gcactggcct
    22681 ggggcgcaac gctaaaaaca attcaggact ggagcgatta ttcaccgatc ctcggtttgc
    22741 ccatcggcct gatgtatgcc gcttgtctgc ccaccagcct cgttatcgca ttctttgaac
    22801 tgcgtcattt atatcaactc atcacgcgta gcaattctct aacgtcgcca ccgcaaggag
    22861 cttaatcatg gctgtgctga tttttctggg ctgtctgttg ggtggtattg ctatcggttt
    22921 gcctattgcc tgggcactgt tgttgtgcgg ggcggcgtta atgttctggc tggacatgtt
    22981 tgatgtccag atcatggcac aaacgctggt gaacggagcc gacagctttt ccctgctggc
    23041 gattccgttc tttgtgctgg cgggtgaaat catgaatgcg ggcggcttgt caaaacgcat
    23101 tgttgacctg ccgatgaaac tggtggggca taaaccaggc gggctgggct acgtcggcgt
    23161 gctggcggct atgattatgg cgagcctttc tggttctgcc gttgcggata ccgccgccgt
    23221 tgccgctcta ctggtgccga tgatgcgcag cgccaactac ccggtcaacc gggcggcggg
    23281 gctgattgcc tctggcggca ttatcgcgcc aattattccc ccttccattc cgtttattat
    23341 cttcggcgtt tccagcggat tatccatcag taagctgttt atggcgggca ttgcccctgg
    23401 catgatgatg ggcgcaacgc tgatgcttac ctggtggtgg caggccagtc gccttaattt
    23461 accccgccag caaaaagcaa cgatgcagga aatctggcac tcttttgtct ccggtatctg
    23521 ggcgctgttt cttccagtca tcattattgg cgggtttcgc tccggtctgt ttacccctac
    23581 cgaagccggg gccgttgctg ctttctatgc gctgtttgtc gccacagtta tttaccgtga
    23641 aatgaccttc gccacacttt ggcatgtact cattggcgcg gcgaaaacca cctcagtggt
    23701 tatgtttctg gtggcctcgg cacaagtttc cgcctggctg attaccattg ctgaactgcc
    23761 gatgatggtt tctgatttac tgcaaccgtt ggtcgattca ccgcgtctgc tgtttatcgt
    23821 cattatggtg gctatcctga ttgtcggcat ggtcatggat ttaacgccga cggtattaat
    23881 tcttaccccg gtattaatgc ctttagttaa agaggcagga atcgatccga tttatttcgg
    23941 tgtcatgttt atcattaact gctcaatcgg cttaattaca ccgcctatcg gtaatgtgct
    24001 taacgttatt tcaggggtgg caaaactcaa attcgatgat gcagtcagag gcgttttccc
    24061 ttacgtcctc gttttatatt ccttattagt cgtatttgtt tttattcccg atctcatcat
    24121 cctccctttg aaatggatta attaaaagga aaatattatg aaattacgct ctgtaaccta
    24181 cgcattattc attgctggcc tggctgcatt cagcacatct tctctggcgg cacaatcatt
    24241 acgtttcggt tatgagacat cacaaaccga ctcgcaacat attgcggcga aaaaattcaa
    24301 tgaattatta caggaaaaaa ccaaaggcga actgaaatta aaattgttcc cggacagcac
    24361 cctcggtaac gcgcaggcga tgatcagcgg cgtacgtggc ggcaccatcg atatggaaat
    24421 gtccggctcg aataactttg ccgggttatc accagtgatg aacttgcttg atgtcccttt
    24481 cctgttccgc gataccgctc acgcgcataa aacgctcgac ggcaaagtcg gtgatgatct
    24541 gaaagcctca cttgaaggta aaggactaaa agtactggcc tactgggaaa acggctggcg
    24601 cgatgtcacc aactcgcgcg caccggttaa aacccccgcc gacctgaaag ggctgaaaat
    24661 tcgcaccaac aatagcccga tgaatatcgc cgcattcaaa gtctttggcg ctaacccgat
    24721 cccgatgccg tttgccgaag tctataccgg gctggaaacc cgcactatcg acgctcagga
    24781 acacccgatc aacgtcgtct ggtcagcaaa atttttcgaa gtgcagaagt acctttctct
    24841 gacgcaccac gcctattccc cgcttctggt ggtgatcaac aaagcgaagt ttgatggctt
    24901 aagcccggag ttccagcaag cgctaatttc atctgcgcag gaagcgggta actaccagcg
    24961 caaactggtt gccgaagatc agcaaaaaat catcgacggc atgaaagaag cgggcgtgga
    25021 agtcatcacc gatctcgacc gcaaagcctt tagcgacgcg ctgggtactc aggtccgcga
    25081 catgtttgtt aaagatgtgc cgcatggcgc tgatctgctg aaagccgtgg atgaggtgca
    25141 ataaaccatg acgcaatact ggctggggtt agattgtggc ggtagctggc tgaaagccgg
    25201 gctgtatgac cgcgaaggcc gggaggcagg cgtgcagcgc ctgccgctgt gcgcattaag
    25261 cccgcagcca ggttgggcag agcgcgatat ggcagaactg tggcaatgct gcatggctgt
    25321 cattcgcgcc ctgcttactc attctggtgt tagtggggaa caaattgtcg gtatcggtat
    25381 ctccgcacag ggaaagggct tgtttttgct ggataaaaac gacaagccgc tcgggaatgc
    25441 tattttgtcc tcggatcgcc gggcgatgga aatcgttcgt cgctggcagg aagatggcat
    25501 cccggaaaaa ctctacccgt tgacccgaca aaccttgtgg accgggcatc cggtgtcgct
    25561 gttacgctgg ctgaaagagc acgaaccgga acgctacgcg caaattggct gcgtgatgat
    25621 gacgcacgac tacctgcgct ggtgtttaac cggcgtcaaa ggctgtgaag agagcaatat
    25681 ttccgagtcc aacctctata acatgagtct tggggaatat gacccgtgcc tcaccgactg
    25741 gctggggatc gctgaaatca atcacgccct gccgcctgtt gtcggttctg ccgaaatctg
    25801 cggggagatc accgctcaga cagccgcact gaccggtctg aaagcgggta cgtccgttgt
    25861 cggcggcctg tttgatgtgg tttccaccgc actctgtgcc gggatcgaag acgaatttac
    25921 ccttaatgcg gtgatgggca cctgggcggt gaccagcggc ataacccgcg gtttacgtga
    25981 cggtgaagcg catccgtatg tctatggtcg ctacgttaac gatggtcaat ttatcgttca
    26041 cgaagccagc cctacctctt ccggcaacct cgaatggttt accgcgcagt ggggagaaat
    26101 ctcgtttgat gagatcaatc aggccgttgc cagcttgccg aaggtcggtg gcgatctctt
    26161 tttcctgccg ttcctgtacg gcagcaacgc cggactcgag atgaccagcg gtttctacgg
    26221 gatgcaggcc attcacaccc gcgcgcacct gttgcaggcc atctatgaag gggtggtgtt
    26281 tagccatatg acccacctca accggatgcg cgaacgtttt actgatgtgc acaccctgcg
    26341 cgtcactggc ggcccggcgc actccgatgt ctggatgcaa atgctggcgg acgtcagcgg
    26401 tctgcgtatc gagctgccgc aggtggaaga aaccggctgt tttggcgcgg cacttgccgc
    26461 ccgcgtcggc accggagttt atcgcgattt cagcgaagct caacgtgact tacagcaccc
    26521 ggtacgcacc ctgctgccgg atatgaccgc gcatcagctt taccaacaaa aataccaacg
    26581 ctatcagcat ctcattgccg cacttcaggg ctttcacgcc cgcatcaagg agcacacatt
    26641 atgagccgac cacttctgca actggcactc gaccactcat cacttgaagc cgcgcagcgc
    26701 gacgtgacgc tgttaaaaga cagcgtcgat atcgtcgaag cgggcaccat tctctgttta
    26761 aacgaagggc ttagcgcggt gaaagccttg cgcgaacaat gcccggacaa aatcatcgtt
    26821 gccgactgga aggtcgccga cgctggtgaa acgctcgcgc aacaggcgtt tggcgcaggc
    26881 gctaactgga tgaccatcat ctgcgccgcg ccgctcgcga cggtggaaaa aggccacacg
    26941 atggcgcaac gctgcggtgg tgaaattcag atcgagttgt tcggtaactg gacgctggac
    27001 gacgcccgcg actggcatcg tattggcgtg cggcaggcga tttatcatcg cggtcgtgat
    27061 gcacaggcca gagggcaaca gtggggcgaa gccgatctgg cactcatgaa ggcgctttcc
    27121 gatatcggcc ttgagctttc cattaccggc gggattactc ctgctgacct tccgctgttt
    27181 aaagatatcc gcgtgaaagc gtttattgcc gggcgggcgc tggcaggcgc agcaaatccg
    27241 gcgcaggtgg ctggcgattt ccatgcgcaa attgatgcta tctggggagg cgcgcgtgcg
    27301 taatcatccg ttaggaattt atgaaaaagc gctggcgaaa gatctctcct ggccggagcg
    27361 gctggtgctg gcaaaaagct gtggctttga ttttgtcgaa atgtcggtgg atgaaaccga
    27421 cgaacggctc tcacgtcttg actggagcgc cgcacaacgt gcttcgctgg tcactgcgat
    27481 gatcgaaacg ggtgttgcca ttccgtcaat gtgcctgtct gcccatcgtc gttttccctt
    27541 tggcagccgg gacgaggcag tgcgcgaacg ggcgcgggaa atcatgagta aagcgattcg
    27601 cctggcgcgc gatctcggca ttcgcaccat ccagctggca ggttatgacg tctattacga
    27661 agaccacgac gaaggcaccc agcaacgttt tgctgaaggg ctggcgtggg cggtcgaaca
    27721 ggcggcggcg tcacaagtca tgctggcggt ggagattatg gacaccgcgt ttatgaactc
    27781 tatcagtaaa tggaaaaagt gggacgagat gctcgcctcg ccgtggttca ccgtctaccc
    27841 ggacgtcggc aacctcagcg cctggggcaa tgatgttccc gccgaactga aactgggcat
    27901 tgaccgtatc gccgcgatcc acctgaaaga cacccagcca gttaccgagc aaagtcccgg
    27961 acagttccgc gacgtgccgt ttggcgaagg ctgcgtcgat ttcgttggca tcttcaaaac
    28021 gctgcatgaa ctgaactatc gcggttcttt cctgattgag atgtggaccg aaaaagccaa
    28081 agagccggtg ctggagatta ttcaggcgcg gcgctggatt gaagcgcgta tgcaggaggc
    28141 cggatttata tgttagagca actgaaagcc gacgtgctgg cggcgaatct ggcgcttccc
    28201 gctcaccatc tggtgacgtt cacctggggc aatgtcagcg cggtagacga aacgcggcaa
    28261 tggatggtaa tcaaaccttc cggcgtcgag tacgacgtga tgaccgccga cgatatggtg
    28321 gtggttgaga tagccagcgg taaagtggtg gaaggcagca aaaagccctc gtccgacacg
    28381 ccaacgcatc tggcgctcta ccgtcgctat gccgaaatcg gtggtattgt gcatacccac
    28441 tcgcgccacg cgaccatctg gtcacaggcc gggctggatc tccccgcctg gggcaccacg
    28501 cacgccgatt atttctatgg tgccatcccc tgcacgcgcc tgatgaccgc agaggagatc
    28561 aacggcgagt atgaatacca gaccggcgaa gtgatcatta aaaccttcga agaacgtggg
    28621 ttgaacccgg cacaaattcc tgcggtgctg gtgcattctc acggcccgtt cgcctgggga
    28681 aaaaatgccg ccgatgcggt gcataacgcc gtggtgctgg aagagtgcgc ctatatgggc
    28741 ctgttctcgc gccagcttgc gccgcaactc cctgccatgc aaaacgaact gctggataag
    28801 cactacctgc gtaagcatgg agacaatgcc tattacgggc agtaatccct cacaccgggg
    28861 cttcatcgcc ccggcactac gaattgatat gttccttgct gtaacgccgt ttccaggcgg
    28921 caggcgttaa accggtgtgc ttgcgaaaaa tctgccggaa atagccgacg tcattaaacc
    28981 cgcacaacgc cgccacttcc gtgagcgatc gcgcttcgct aattaacagc ttttcggctt
    29041 cgcgcacccg ctggcggtgg attgcttctg ttaacgtcag atgaaacacc cgccggtaga
    29101 cgcgcccaag ataatcggca ttgcagtaca gctcacgggc taaaagtgcc gtggaaagcg
    29161 ggcggtgata ttgcgtgcga atcagtttct gcgctttcca ggcaagcgtg gtacctgcgg
    29221 catcggcctg ttcatcaaga cgcgcgtcac gggtgagctg ctgcaaaatc agcagcacaa
    29281 taaactccag cgccggagtg cgttgatgct tttcctgttc gcttaagaac tggcgaaaca
    29341 gggccaccag cgcctgcgga tcggcaacct tcgtttgttg cggcacgcta agatgagtga
    29401 gccagcgcgg gtcgctatcc ggcgctttaa cctcaaaatg cagccagtaa aatttgagat
    29461 cggcgggaaa atcctccacc ccaacatgtt gtcgattcgg ccagagtaat aaactctctc
    29521 ccgcatcgac cagaaatatc cgttcttctt cctgaatcgt taatcgacct ttttcgacaa
    29581 agataatttc ccatgactcc agccgccttg caggatgcct ccctacgccg cgggaaataa
    29641 ataatccacc attttgcact ttaatcggta acgtcatgga taattcgagc attgatatgt
    29701 aatttcccgc ttattcatta atgattaaac acattacctt ttgattatta atcaaaaaca
    29761 ttaatttcac ctgatccatt tcacactttt aaattcaggt cagaatcgtc accttttttt
    29821 actcttttct cgccttgttg atattcccgt tcacggcaga attaatcagg tcatttcaat
    29881 aaaactaaaa attgcttcct attcacgagg aatatctatg acctctacac cgattactac
    29941 cgctgatatc gctaaaggta aaattgacga tgcgttatct gtacgggaaa aaataggcta
    30001 cggcctgggt gacgctggcg gcaccgtaat aacttgcctg atcatgaatt ttctcacctt
    30061 tttctacacc gacgtttttg gattaactcc ggcgctggtt ggcacgctgt ttattgcact
    30121 gcgcgtgttt gatgccatct ccgacccggt gatgggcgtc attgccgacc ggacgcaaag
    30181 ccgatggggg cgctttcgtc cgtggcagct atggattgcc attcccatcg gcattatcgg
    30241 catcctgacg ttcaccgtgc cagatgccag catgggagta aaaatcgcct gggcgttcgg
    30301 tacttacctg ctcctttcag tcggttatac cgccatcaac gtaccgtact gcgcgctgat
    30361 caacaccatg accacccgcc acaatgaagt gatctcctgc cagtcctggc gattcgttct
    30421 ctgcggcgta gcgggatttc tggtttcggt aggcttaccg tggatggtag atctcttcgg
    30481 tcagggcaac gctgcacgcg gctatcaact gggcgtcggc gtattgtgcg ccattgccgt
    30541 ggtgatgttc ctgtgctgtt ttttctgggt tcgtgaacgg gtgccgctct ccacaatggg
    30601 gaaatttacc ctgcgcgaac atcttgccgg gcttcggaac aacgaccaac tgctgctgat
    30661 gctggtcatg tctttcctgc tgattaacgt ctttaatatt cgcggcggtg ggtatatgta
    30721 cttcattacc tacgtcttac aaggcagtac gggctacaca tcgctgttct tcaccatggt
    30781 caccttcgcc tccattatcg gctcggtgat tgtcagcccg ttaacgcggc gtttcgatac
    30841 cgtcaaaatt tattactaca ccaacctgct cctcgctgcg ctggcggtgt tgatgtggtt
    30901 cctgccctcc ggcccggctt atcaaacgct gtggctggcg gtgatcctcg gtaatggcgt
    30961 gattcttggc ttcacattgc cattgcactt ctcattgatg gcctttgccg atgactacgg
    31021 cgagtggaaa acccgcgtac gttcttccgg catgaacttc gccttcaatc tgtttttcat
    31081 caagctggcc tgggcctcca gcgccgggat catcagcctg ctgtttattt ttgtcgccta
    31141 ccagcctggc gtggaaaacc agaccgccag ttcgcttggc gggatcgcgg cgatggaaac
    31201 attactgcct gcgctattcc acctgctgct ggcaggggcg atccgctttt gcaaactcaa
    31261 taatcctatg atgtcacgca tagctaccga cctgcgtcag cgtcatgtac agccttaagg
    31321 agcacaagat gaacatttcg gaagtcgatc tgcgtaaact gacggtcagc gatccgttcc
    31381 tcggtcagta ccaacaactg gtccgcgacg tggtgattcc ttatcagtgg gatgccttga
    31441 acgatcgtat cccagaagcg gaacccagcc atgcgattga aaactttcgc attgctgccg
    31501 gacttcagga gggtgaattt tacgggatgg tgtttcagga cagcgacgtc gccaaatggc
    31561 tggaagcggt agcctggtcg ctgtgccaga agccggacgc cgaactggaa aaaaccgccg
    31621 acgaggtaat cgaactgatc gcctccgccc aatgcgaaga cggctatctc aatacttact
    31681 ttacggtaaa agcacccgaa gaacgctgga gcaatctggc ggagtgtcat gaactttact
    31741 gcgcaggtca tctgattgaa gccggagtcg ccttcttcca ggccacggga aaacgacgct
    31801 tgctggaggt ggtttgccgt ctggccgatc atatcgaccg cgtatttggt ccagatgaag
    31861 acaagctgca gggttatccc ggtcatccgg aaattgaact ggcgctgatg cgtctctacg
    31921 aagtcaccga agagccgcgc tacctggcgc tgacgaacta ttttgtcgaa cagcgtggtg
    31981 cgcaaccgca ctattacgac caggaatatg aaaagcgcgg gcagacctcg cactggcaca
    32041 cctacggccc ggcgtggatg gtgaaagaca aagcctacag ccaggcacat ttgccccttg
    32101 cgcaacagca aaccgccatc ggtcacgcgg tacgttttgt ctacctgatg accggcgtcg
    32161 cgcatctcgc gcgtttaagt cacgatgaca gcaagcgtca ggactgcctg aggctgtgga
    32221 acaatatggc ccagcgtcag ttatatatta ccggcggcat tggctcgcaa agcagcggcg
    32281 aagcgttcac tagcgattac gatctgccga atgacacggt ctacgccgaa agttgtgctt
    32341 ccatcggcct gatgatgttc gcccggcgaa tgctggaaat ggaaggcgac agtcaatatg
    32401 ccgatgtgat ggagcgcgcg ctgtacaaca ccgtgctcgg cggcatggcg ctggatggca
    32461 aacatttctt ctatgtgaat ccgctggaag tacatccaaa atcgctgaaa ttcaaccata
    32521 tctacgatca cgttaaaccg atccgccagc gttggtttgg ttgcgcttgt tgtccgccaa
    32581 atatcgcccg cgtgctgacc tcgattggtc attatctcta cacgccgcgt gaagatgcgt
    32641 tgtatatcaa catatacgca ggaaacagca tggaagtgcc ggtagaaaat ggcacgctgc
    32701 gcctgcgggt tagcgggaac tatccgtggc aggagcaggt gacgattgcg gttgaatcgc
    32761 cccagccggt acgtcatacg ctggctttac gtctgccgga ctggtgcaca cagccgcaga
    32821 tcatattgaa tggggaagag gtcgagcagg atattcgtaa agggtatttg cacattaccc
    32881 gcgaatggca ggagggcgac acgctgaatc tgactttgcc aatgccggta cgtcgcgttt
    32941 acggtaaccc gctggtgcgt cacgtcgccg gaaaagtggc gattcagcgc ggtccgctgg
    33001 tgtattgcct ggaacaggcc gacaacggcg agtcactgca taatctgtgg ctgcccgccg
    33061 atgcgccatt tacgacattt gaaggcaagg gattgtttag ccataagatc ttaatccagg
    33121 ctccgggtta ccggtatgaa cagagcaatc cagagcagca accgctgtgg cattacgaca
    33181 gcgcgccagc caaacgccag acgcaaactc tgacatttat cccgtggttt agctgggcca
    33241 accggggtga aggcgaaatg cggatctggg tgaatgagga aaccagcgca tgatgcgacg
    33301 cttaacgcgt cttatcagta tcaacatatc ggtgcggttg cccgccaacc atcgaaacgc
    33361 gggattaagc gcccggcaat acacgccggg cagcttccgc cagaaacgca cttctgttgg
    33421 cgtagtctgg gttattgcgc acgtaggtat caatcttgtt cagtaatctt tttggcatcg
    33481 taatattgat acgttcagcc cgaccttcga actgattcat attgatatcc accagcaacc
    33541 attgcccgcc aataaagtcc tgcgcacgct ggaccagatg tgtttccaca gagcctggaa
    33601 gcggcaactc ttcattcatc tcgcataagg tttcgaagtg cgcgaccagt gcacttcgcg
    33661 catcctggaa agcgttgtcc agcgtatcac cagcaaaata acagcctgga acatcaggga
    33721 aaaaaccgct ggcgctgccg tcaggatcac tgtggatata ggcgggataa aacatattgc
    33781 ctcctgaaat tttaaaatgt taaaccagcc tgacgcccaa tttgcgccag cgtaccgggc
    33841 ttaatatctt tttagggtgc ggaaccgtca ccagacccgg atgaacaggg tggcggaact
    33901 gatgatgact gccacgggtt cgaatatgtt cccagccatg ctgcttcagg atggcgatca
    33961 cgtctgcact tttcacatcc ctgcccttcc atgattatac acacgataca cacacaatta
    34021 gcaataacaa tcacgatagc gcgatttata tgggccgtca gggggataaa gttgactcag
    34081 gaatgccgct cggaaggaga ataacggtgt gcagtaactt gttgaaaaga tgcgaggaga
    34141 ctcgtaatta agttcaatca atattcagtc attcaagacg tagacatttg aattggtaaa
    34201 atgcgcaaac gtcgcatcag gcaatgaata cccaatgcga ccagcttctt atatcagaac
    34261 agccccaacg gtttatccga gtagctcacc agcaggcact tggtttgctg gtaatgctca
    34321 agcatcattt tgtgggtttc acgaccgatg cctgattgtt tgtagccacc aaacgccgca
    34381 tgtgccgggt aagcgtgata gcagttggtc cacacgcgcc cggcctgaat gccgcgcccc
    34441 atcttatagg ccagattacc gttgcggctc cagacacctg cgcccaggcc atattgcgtg
    34501 tcgttcgcca gctccagcgc ttcttccatc gttttgaagg tggtcaccgc cagcaccggg
    34561 ccaaaaattt cttcctggaa cacgcgcata ttgttctgac caaacagaat cgtcggttcg
    34621 aggtagtagc cgtctttcag ttcaccttcc agcagcttgc gccgcccgcc ggtgagcacg
    34681 tctgcgccct cttttttacc gatatcaatg tagttgagga tggtttccag ttgcccgtga
    34741 gaaacctgcg cacccatttg cgtcacgctg tcgagcgggt taccgctacg aatgctttcg
    34801 acacggcgga tggcgcgttc cataaagcgt tcgtagatag attcctgcac taaagcacgg
    34861 cttggacagg tgcaaacttc gccctggtta aaggcaaaca gtgcaaagcc ttccagcgct
    34921 ttatcgaaaa aggcatcttc ttcatccatc acgtcagcaa agaagatatt tggcgatttg
    34981 ccgcccagct ccagcgtcac cggaataatg ttttgcgtgg cgtattgcat aatttgttgg
    35041 cccacttccg ttgagccggt aaacgccact ttggcgatgc gtttcgaggt cgccagatat
    35101 tcgccaattt ccccacctgc gccattgacc acgttcacca cgcccggcgg cagtaaatca
    35161 ccgacaattt ccattagcag cagtacagaa agcggggtaa gacgtgcggg tttcagcacc
    35221 acacagttgc ccgccgccag cgcgggagcc attttccagc tcgccatcag cagcgggaag
    35281 ttccacggga taatctgccc caccacgcct aacggttcat ggaaatgata ggccacggtt
    35341 tcgctatcaa cttcactgat cccaccttcc tgcgcccgaa tacacgaggc gaaatagcgg
    35401 aaatggtcaa tcgccagcgg tacatccgca gcactggttt cgcgaatggg tttgccgtta
    35461 tcccaggttt cagctgtcgc taacagctcg aggttttgtt ccattcgatc ggcaatctta
    35521 aacagaatcg ccgcgcgatc ctgcaccgag gtgtgcgccc atttatcttt cactttgtgc
    35581 gcagcatcca gcgccagatc gatgtctcgt ttgcccgaag acgccacttc gcacagcagc
    35641 tgcccggtca ccggcgtcag attctggtaa tactcgccgt cggcaggggc tacccattcg
    35701 ccgccaataa agttgtcata gcgggctttt aacttgaggg ggaaaccata ctcgccgggc
    35761 ttaatctgtg ctgaaggggg attattggtc atgatcgtct ccttgcggtg tgaggtataa
    35821 caagggtaga cgtgactggc gaaatcttcg ccagtcggta acagctttac gacagctatc
    35881 acgaatttac gggcaagggt tacagagata gttgaaaaag cggctgacaa tttgccagcc
    35941 gttgtggaaa tgatgattac atcgctgcgc gataaatcgc cacaatttct tcgtgagttg
    36001 cctggatcgg gttagtaaaa ccacaggcat ctttcagggc attagtcgcc agaaccgcga
    36061 aatcttcttc tttcacgttc aggtcgcgta ggcctgccgg gatatccact ttcttcgcca
    36121 gttcacggat ggcgttaatg caggcttcag caccttccgc atcgttttta cctgtcacgt
    36181 tcacgcccat tgcagcggca cagtcacgca gacgtgcggc ggcgactttg ctgttgaata
    36241 cctgaacatg cggcagcaaa acggcgttac atacaccgtg tggcaggttg tagaaaccgc
    36301 ccagctggtg cgccatcgca tgaacataac ccagagaagc attattgaac gccataccgg
    36361 cgaggaactg ggcataagcc attgcttcac gcgctttcgc attactgcca tcttcaacgg
    36421 ctaacggcag gttttcggca atcatggtca cggctttcag tgcacaagcg tcagtgatcg
    36481 gcgtggcggc aatagaaaca tacgcttcga tagcgtgcgt taaggcatcc atacccgttg
    36541 cggcggtcag tgacttcggc ataccaatca tcagagagga gtcattgaca gaaagcagcg
    36601 gagtgacatg tttatcaaca atcgccattt tgatatgacg cgcttcgtca gtgatgatgc
    36661 agaaacgggt catttcagag gccgtacccg ccgtggtatt gatggcgatc atcggcagct
    36721 gcggttttgc agagcggtca acgccttcgt aatcgcgaat atcgccgcca ttggctgcca
    36781 ccagcgcaat accttttgcg cagtcgtgtg gagaaccacc gcctaaggag atcacgctat
    36841 cgcaattatt ctctttaagt aatttcaaac ctgcggcgac gttttccgtg gtggggttag
    36901 gttgggtgcc atcataaata acgctaaaaa tattgcgttc ttccagtgct ttttgcacat
    36961 cgcccgccat acctaatttc gttaacatac tgtcagtgac aattaaggta cgggtaaatc
    37021 cataatctgc catcatattc attgcatcag tcaatgaatc agcgccgatg acattcacag
    37081 aaggaataaa gaaagttgaa gatgccataa cacactcctg aaagtgtaaa aaagggtggg
    37141 cagcttactg aggattttca ttcgaaaaca tgatcattat tattgattat caaaattaat
    37201 ctaataaaaa gcacattgta ttcattaaac aatg
//
LOCUS       DAZGXZ010000035        37111 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000035 DAZGXZ010000000
VERSION     DAZGXZ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37111
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.035"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            5..736
                     /locus_tag="ACHKJF_001874"
     CDS             5..736
                     /locus_tag="ACHKJF_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949243.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGMDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            872..1792
                     /gene="speB"
                     /locus_tag="ACHKJF_001875"
     CDS             872..1792
                     /gene="speB"
                     /locus_tag="ACHKJF_001875"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP0949244.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDIDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(1998..2756)
                     /gene="loiP"
                     /locus_tag="ACHKJF_001876"
                     /gene_synonym="yggG"
     CDS             complement(1998..2756)
                     /gene="loiP"
                     /locus_tag="ACHKJF_001876"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP0949245.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKALSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            3034..5025
                     /gene="tkt"
                     /locus_tag="ACHKJF_001877"
     CDS             3034..5025
                     /gene="tkt"
                     /locus_tag="ACHKJF_001877"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0949246.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTNFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            5339..5782
                     /gene="cmtB"
                     /locus_tag="ACHKJF_001878"
     CDS             5339..5782
                     /gene="cmtB"
                     /locus_tag="ACHKJF_001878"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP0949247.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            5810..7198
                     /gene="cmtA"
                     /locus_tag="ACHKJF_001879"
     CDS             5810..7198
                     /gene="cmtA"
                     /locus_tag="ACHKJF_001879"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP0949248.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMTKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            7213..8490
                     /gene="yggP"
                     /locus_tag="ACHKJF_001880"
     CDS             7213..8490
                     /gene="yggP"
                     /locus_tag="ACHKJF_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0949249.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            8487..9452
                     /gene="glpX"
                     /locus_tag="ACHKJF_001881"
     CDS             8487..9452
                     /gene="glpX"
                     /locus_tag="ACHKJF_001881"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0949250.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            9474..9983
                     /gene="fumE"
                     /locus_tag="ACHKJF_001882"
     CDS             9474..9983
                     /gene="fumE"
                     /locus_tag="ACHKJF_001882"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP0949251.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            9980..10693
                     /locus_tag="ACHKJF_001883"
     CDS             9980..10693
                     /locus_tag="ACHKJF_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP0949252.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10665..11342)
                     /locus_tag="ACHKJF_001884"
     CDS             complement(10665..11342)
                     /locus_tag="ACHKJF_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0949253.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKFPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(11336..12013)
                     /locus_tag="ACHKJF_001885"
     CDS             complement(11336..12013)
                     /locus_tag="ACHKJF_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0949254.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(12001..12708)
                     /locus_tag="ACHKJF_001886"
     CDS             complement(12001..12708)
                     /locus_tag="ACHKJF_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP0949255.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(12709..13287)
                     /locus_tag="ACHKJF_001887"
     CDS             complement(12709..13287)
                     /locus_tag="ACHKJF_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP0949256.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTNPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(13310..13741)
                     /locus_tag="ACHKJF_001888"
     CDS             complement(13310..13741)
                     /locus_tag="ACHKJF_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP0949257.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            14113..15132
                     /gene="epd"
                     /locus_tag="ACHKJF_001889"
     CDS             14113..15132
                     /gene="epd"
                     /locus_tag="ACHKJF_001889"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP0949258.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            15182..16345
                     /gene="pgk"
                     /locus_tag="ACHKJF_001890"
     CDS             15182..16345
                     /gene="pgk"
                     /locus_tag="ACHKJF_001890"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP0949259.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            16560..17639
                     /gene="fbaA"
                     /locus_tag="ACHKJF_001891"
     CDS             16560..17639
                     /gene="fbaA"
                     /locus_tag="ACHKJF_001891"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP0949260.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(17703..17801)
                     /locus_tag="ACHKJF_001892"
                     /pseudo
     CDS             complement(17703..17801)
                     /locus_tag="ACHKJF_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17997..18857
                     /gene="mscS"
                     /locus_tag="ACHKJF_001893"
     CDS             17997..18857
                     /gene="mscS"
                     /locus_tag="ACHKJF_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0949261.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            18996..19631
                     /gene="argO"
                     /locus_tag="ACHKJF_001894"
     CDS             18996..19631
                     /gene="argO"
                     /locus_tag="ACHKJF_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP0949262.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            19724..20464
                     /gene="yggE"
                     /locus_tag="ACHKJF_001895"
     CDS             19724..20464
                     /gene="yggE"
                     /locus_tag="ACHKJF_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP0949263.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            20631..21527
                     /gene="ygfI"
                     /locus_tag="ACHKJF_001896"
     CDS             20631..21527
                     /gene="ygfI"
                     /locus_tag="ACHKJF_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0949264.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(21524..23002)
                     /gene="scpC"
                     /locus_tag="ACHKJF_001897"
     CDS             complement(21524..23002)
                     /gene="scpC"
                     /locus_tag="ACHKJF_001897"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP0949265.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGEIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(23026..23811)
                     /gene="scpB"
                     /locus_tag="ACHKJF_001898"
     CDS             complement(23026..23811)
                     /gene="scpB"
                     /locus_tag="ACHKJF_001898"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP0949266.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(23822..24817)
                     /gene="meaB"
                     /locus_tag="ACHKJF_001899"
     CDS             complement(23822..24817)
                     /gene="meaB"
                     /locus_tag="ACHKJF_001899"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP0949267.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(24810..26940)
                     /gene="scpA"
                     /locus_tag="ACHKJF_001900"
                     /pseudo
     CDS             complement(24810..26940)
                     /gene="scpA"
                     /locus_tag="ACHKJF_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(27144..28037)
                     /gene="argP"
                     /locus_tag="ACHKJF_001901"
     CDS             complement(27144..28037)
                     /gene="argP"
                     /locus_tag="ACHKJF_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP0949268.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28179..28364
                     /locus_tag="ACHKJF_001902"
     CDS             28179..28364
                     /locus_tag="ACHKJF_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949269.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            28420..29079
                     /gene="rpiA"
                     /locus_tag="ACHKJF_001903"
     CDS             28420..29079
                     /gene="rpiA"
                     /locus_tag="ACHKJF_001903"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP0949270.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29335..30567
                     /gene="serA"
                     /locus_tag="ACHKJF_001904"
     CDS             29335..30567
                     /gene="serA"
                     /locus_tag="ACHKJF_001904"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0949271.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            30796..30855
                     /gene="ibsC"
                     /locus_tag="ACHKJF_001905"
     CDS             30796..30855
                     /gene="ibsC"
                     /locus_tag="ACHKJF_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP0949272.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            31188..31247
                     /locus_tag="ACHKJF_001906"
     CDS             31188..31247
                     /locus_tag="ACHKJF_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0949273.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(31348..31950)
                     /gene="fau"
                     /locus_tag="ACHKJF_001907"
     CDS             complement(31348..31950)
                     /gene="fau"
                     /locus_tag="ACHKJF_001907"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP0949274.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(31971..32154)
                     /gene="ssrS"
                     /locus_tag="ACHKJF_001908"
     ncRNA           complement(31971..32154)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKJF_001908"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(32196..32525)
                     /gene="zapA"
                     /locus_tag="ACHKJF_001909"
     CDS             complement(32196..32525)
                     /gene="zapA"
                     /locus_tag="ACHKJF_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0949275.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            32693..33271
                     /gene="ygfB"
                     /locus_tag="ACHKJF_001910"
     CDS             32693..33271
                     /gene="ygfB"
                     /locus_tag="ACHKJF_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP0949276.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            33297..34622
                     /gene="pepP"
                     /locus_tag="ACHKJF_001911"
     CDS             33297..34622
                     /gene="pepP"
                     /locus_tag="ACHKJF_001911"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP0949277.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            34619..35797
                     /gene="ubiH"
                     /locus_tag="ACHKJF_001912"
                     /gene_synonym="visB"
     CDS             34619..35797
                     /gene="ubiH"
                     /locus_tag="ACHKJF_001912"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP0949278.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            35820..37022
                     /gene="ubiI"
                     /locus_tag="ACHKJF_001913"
                     /gene_synonym="visC"
     CDS             35820..37022
                     /gene="ubiI"
                     /locus_tag="ACHKJF_001913"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP0949279.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
BASE COUNT         8842 a         9281 c         9973 g         9015 t
ORIGIN      
        1 ggttatgaaa aaatggaaag tacgtagcgc gttggtggca ttaatcgtgc tgctggccgg
       61 ttgttccagt aatgcgcagt ataatagcag tgcatcgggc aatgtcggca ccgcctgggg
      121 tggggatgtg cactcatcgg tccagggcgt atctgctgaa cgagcctggc gcgatcctgc
      181 agagatgatc gttattagtt attcgactaa tgttccttcc ggctatgacc gcgtttatag
      241 cattcgtatt aacgaactgg aatatgctat tcgcgatggt aattttaatt ctctacctat
      301 tacgcgtgtt tatgattcat ccaataatga accacgctat attgtccatg ccagagtcgg
      361 aatgaattat caattatatg tccgtaatta cagtcgtaac accaactacg agatcgttgc
      421 tacggttgat ggcatggatg tgctgaacgg caagcaaggt tcgctgaata acaacggcta
      481 tattgtgaat gccggagatt cgttagcgat taaaggattc cgcaaagata aacataccga
      541 agcggctttt cagttcgcca acgttgctga ttcctacgca gccaattcgg cgcaaggcga
      601 tgtgcgtaat accggggtaa tcggctttgc cgcctttgaa ttacaaggcc cggcgcaaaa
      661 cgcgctgccg ccgtgctctg gacaagcctt tccggcagat aacaatggct atgcgccgcc
      721 accgtgtcgg aaatagcaac ctcgcttgat gcctcgccca tttaaggcga taattagcac
      781 aaacttgtgt ttcgcaaatc caacccttcc tcgtcgggcc taacgacgcg gaagggtttt
      841 tttatatcga ctttgtaata ggagtccatc catgagcacc ttaggtcatc aatacgataa
      901 ctcactggtt tccaacgcct ttggtttttt acgcctgccg atgaacttcc agccgtatga
      961 cagcgatgct gactgggtga ttactggtgt gccgttcgac atggccacct ctggccgtgc
     1021 cggtggtcgc cacggtccgg cagcgatccg tcaggtttcg acgaatctgg cctgggaaca
     1081 caatcgcttc ccgtggaatt tcgacatgcg tgagcgtctg aacgtcgtgg actgcggtga
     1141 tctggtatat gctttcggcg atgcccgtga gatgagcgag aagctgcagg cgcacgccga
     1201 gaagctgctg gctgccggta agcgtatgct ctccttcggt ggtgaccact ttgttacgct
     1261 gccgctgctg cgtgctcatg cgaagcattt cggtaaaatg gcgctggtac actttgacgc
     1321 ccacaccgat acctatgcga acggttgtga atttgaccac ggcaccatgt tctataccgc
     1381 gccgaaagaa ggtctgatcg acccgaatca ttccgtgcag attggtattc gtaccgagtt
     1441 tgatatagac aacggcttta ccgtgctgga cgcctgccag gtgaacgatc gcagcgtgga
     1501 tgacgttatc gcccaggtga aacagattgt gggtgatatg ccggtttacc tgacctttga
     1561 tatcgactgc ctggatcctg cttttgcacc aggcaccggt acgccagtga ttggcggcct
     1621 gacctccgat cgcgctatta aactggtacg cggcctgaaa gatctcaaca tcgttgggat
     1681 ggacgtagtg gaagtggctc cggcatatga tcagtcggaa atcaccgctc tggctgcggc
     1741 gacgctggcg ctggagatgc tgtatattca ggcggcgaaa aagggcgagt aagcaccaga
     1801 tgcgatgcgc acgggtaaaa cgtgccatta atgtcggatg cggcgtgaac gccttatccg
     1861 acctacgttc ggcacccgta ggccggataa gatgcgccag catcgcatcc ggcaatgcgc
     1921 acaaggtaac aaatgtgcca ttcatgtcag atgcggcgtg aacgccttat ctgacctacg
     1981 ttcgacacca ccaggcttta cttaatccca tccgcgctca tgcgatcgcg aatatgctgg
     2041 gcgcgttcgg cggatgcagg atggtcgtca aacattgagc tttggcggcc ttcttccagt
     2101 ttcgccagtt tttcaaagct ggtggcaaga cctgccgggc tgatgccgcg ttggcgcaga
     2161 agatcgtaag aataatcatc ggcttctgct tcctggcgct gggagaattg cgaattgact
     2221 aatttctcgc ccagatcacc aagttgtgat tgagataaac tgccgacaat cccgcccgca
     2281 gaggccgcag ctactcgcac ggcatttgta ccaagcgcca cctgcatccc tttcttcaca
     2341 tggcctaacg ccacgtgccc catctcgtga ccgatcaccg cttcgacttc gttatccgtc
     2401 atcatatcca tcagcccgct atagacgcgg atacagccgt tagccattgc aaaggcgttc
     2461 acatccttcg ccatatacac tttgtaattc accggctgac cgttgatatt gttgcctagc
     2521 gcattggcaa tagttgtcag acgtttagcg tattcgctat tggctggcgc aatcgtcgcc
     2581 ttgctgtcca tctcctgaca tgcctgatcg ctcagggctt tcacctgcgc atcactcaaa
     2641 ctgtaagcct gaaaagcttc cgctcctgat gaaagcagtc cgttggagtc catattctgg
     2701 caaccggtca gtaccgttgc cacgctcatt gctaccaata aggcgcgaat tttcatgttt
     2761 tctcttccgc acaaggtcag gattattcta aaaatgcgcc gtttgcaggt gaatcgacgc
     2821 tcagtctcag tataaggaaa agcgcagcgg acgggcgagt agattgcgca acatgcgagc
     2881 atgatccaga gatttctgaa gcggcaaaag gatgttccat gtacatgacg cgcggcttgc
     2941 ggtaaattgt tggcaaattt tccggcgtag cccaaaacgc gctgtcgtca agtcgttaag
     3001 ggcgtgccct tcatcatccg atctggagtc aaaatgtcct cacgtaaaga gcttgccaat
     3061 gctattcgtg cgctgagcat ggacgcagta cagaaagcca aatccggtca cccgggtgcc
     3121 cctatgggta tggctgacat tgccgaagtc ctgtggcgtg atttcctgaa acacaacccg
     3181 cagaatccgt cctgggctga ccgtgaccgc ttcgtgctgt ccaacggcca cggctccatg
     3241 ctgatctaca gcctgctgca cctcaccggt tacgatctgc cgatggaaga actgaaaaac
     3301 ttccgtcagc tgcactctaa aactccgggt cacccggaag tgggttacac cgctggtgtg
     3361 gaaaccacca ccggtccgct ggggcagggt attgccaacg cagtaggtat ggcgattgca
     3421 gaaaaaacgc tggcggcgca gtttaaccgt ccgggccacg acattgtcga ccactacacc
     3481 tacgccttca tgggcgacgg ctgcatgatg gaaggcatct cccacgaagt ttgctctctg
     3541 gcgggtacgc tgaagctggg taaactgatt gcgttctacg atgacaacgg tatctctatt
     3601 gatggtcacg ttgaaggctg gttcaccgac gacaccgcaa tgcgtttcga agcttacggc
     3661 tggcacgtta ttcgcgacat cgacggtcat gacgcggcat ccatcaaacg cgcagtagaa
     3721 gaagcgcgcg cagtgactga caaaccgtcc ctgctgatgt gcaaaaccat catcggtttc
     3781 ggttccccga acaaagccgg tactcacgac tcccacggtg cgccgctggg cgacgctgaa
     3841 attgccctga cccgcgaaca gctgggctgg aaatatgcac cgttcgaaat cccgtctgaa
     3901 atctatgctc agtgggatgc gaaagaagca ggccaggcga aagaatccgc atggaacgag
     3961 aaattcgctg cttacgcgaa agcttatccg caggaagccg ctgaatttac ccgccgtatg
     4021 aaaggcgaaa tgccgtctga cttcgacgcg aaagcaaaag agttcattgc taaactgcag
     4081 gctaacccgg cgaaaatcgc cagccgtaaa gcgtctcaga atgctatcga agctttcggt
     4141 ccgctgttgc cggaattcct cggcggttct gctgacctgg caccgtctaa cctgaccctg
     4201 tggtctggtt ctaaagcaat caacgaagat gctgcgggta actacatcca ctacggtgtt
     4261 cgcgagttcg gtatgaccgc gattgctaac ggtatctccc tgcacggtgg tttcctgccg
     4321 tacacctcca ccttcctgat gttcgtggaa tacgcacgta acgccgtacg tatggctgcg
     4381 ctgatgaaac agcgtcaggt gatggtttac acccacgact ccatcggtct gggcgaagac
     4441 ggcccgactc accagccggt tgagcaggtc gcttctctgc gcgtaacccc gaacatgtct
     4501 acatggcgtc cgtgtgacca ggttgaatcc gcggtcgcgt ggaaatacgg tgttgagcgt
     4561 caggacggcc cgaccgcact gatcctctcc cgtcagaacc tggcgcagca ggaacgaact
     4621 gaagagcaac tggcaaacat cgcgcgcggt ggttatgtgc tgaaagactg cgccggtcag
     4681 ccggaactga ttttcatcgc taccggttca gaagttgaac tggctgttgc tgcctacgaa
     4741 aaactgactg ccgaaggcgt gaaagcgcgc gtggtgtcca tgccgtctac cgacgcattt
     4801 gacaagcagg atgctgctta ccgtgaatcc gtactgccga aagcggttac tgcacgcgtt
     4861 gctgtagaag cgggtattgc tgactactgg tacaagtatg ttggcctgaa cggtgctatc
     4921 gtcggtatga ccaacttcgg tgaatctgct ccggcagagc tgctgtttga agagttcggc
     4981 ttcactgttg ataacgttgt tgcgaaagca aaagaactgc tgtaattagc atttcgggta
     5041 aaaaggtcgc ttcggcgacc ttttttatta ccttgatatg tccgtttgcg gacaagcaat
     5101 agataaagcg tgttgtagat cacaaatatt tatatgcaat aaatatcaat tatgtaatat
     5161 gcatcacgat atgcgtattg acatttgttg ttatatctat aactcaatgt tatataagaa
     5221 attaataatt cactgttttc aaaacatcgg tttccctgct caattgcttt cattaaaccg
     5281 cacgagtatt ttatttttac cgccggacgg taccctaaaa ataaaataag gataatttat
     5341 gcggcttagt gattattttc cagaatcatc aatctcagtt atacattcag caaaagattg
     5401 gcaggaagct atcgatttct cgatggtatc attgctggat aaaaactata tcagcgagaa
     5461 ttacattcag gcaattaaag attccaccat taacaatggc ccttattata ttctcgcacc
     5521 aggcgtggca atgcctcatg cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt
     5581 gacattactt gaacaaggtg tttattttcc ggggaatgac gaaccaatta aattactcat
     5641 cggactctct gctgccgatg ccgattcgca cattggcgct attcaggcgt taagtgaatt
     5701 actgtgcgaa gaagaaatac tcgaacaact cttaacagca tcatcagaaa aacaattagc
     5761 ggacattatc agccgcggat aattactctc tcctctacag gataatatta tggaaaacaa
     5821 gtctgctcgt gcaaaggtcc aggcttttgg gggctttttg actgcaatgg tcatccccaa
     5881 tattggtgct tttattgcct ggggcttcat aactgcgtta tttattccca ccggttggct
     5941 gccgaatgaa catttcgcca aaattgtcgg cccgatgatt acctatttat tgcccgtgat
     6001 gattggttct actggtggtc atctggtcgg cggtaaacgc ggggcggtca tgggcggaat
     6061 aggtactatt ggtgtgatcg ttggcgcaga gatcccgatg ttccttggct caatgatcat
     6121 ggggccgctc ggtgggttgg tcataaaata tgtcgataag gcactggaaa aacgcatacc
     6181 tgccggtttt gagatggtta tcaataactt ctcattaggt atcgcgggga tgctcctttg
     6241 tctgctgggt tttgaagtga tcggcccggc ggtgttaatt gccaatactt tcgtcaaaga
     6301 gtgtatcgag gcgctggtac atgcgggtta tctgcctctg ctgtcagtca tcaatgaacc
     6361 ggcgaaagtg cttttcctta ataatgcgat cgatcagggc gtctattatc cgctgggaat
     6421 gcaacaggct tcggttaacg gtaaatccat cttctttatg gtggcctcta acccaggtcc
     6481 gggcctgggg ctgctgctgg cgtttacctt gtttggtaaa gggatgacta aacgttctgc
     6541 gcccggggcg atgattattc acttcctcgg tgggatccac gaactgtatt tcccatatgt
     6601 gctgatgaag ccgctgacta ttattgccat gattgcgggc ggtatgtctg gtacctggat
     6661 gtttaactta ctggacggtg gtctggtggc tggcccaagt ccggggtcta tctttgctta
     6721 cctggcactg acgccgaaag gctcgttcct ggcgacaatt gccggtgtta cggtaggtac
     6781 cctggtgtcc tttgctatta cttcgctgat actgaagatg gaaaaaacgg tggaaacgga
     6841 gagcgaagat gagtttgctc agtcagccaa tgcggttaag gcgatgaaac aagagggtgc
     6901 attctcgtta agcagggtta agcgtatcgc ctttgtttgc gatgcgggga tgggctccag
     6961 tgcgatgggc gcgaccacct tccgtaaacg cctggaaaaa gcggggctgg caatagaagt
     7021 aaaacattac gcgatagaaa acgtgcctgc ggatgccgat atcgtcgtca ctcacgccag
     7081 tctggaaggg cgcgtgaaac gtgtgacgga taaaccgctg atattgatta ataactatat
     7141 tggcgatcca aaactcgaca ctttatttaa tcaattaacc gccgaacata aacactgatt
     7201 acagaggtaa aaatgaaaac caaagttgct gctatttatg gcaagcggga tgtccgtctg
     7261 cgcgaatttg aactgccaga aattaccgat aatgaactgt tagtgagtgt aatttctgac
     7321 agcgtctgtt tatcgacctg gaaagcggcg ttactcggta gtgaacataa acgcgtaccc
     7381 gacgatttag aaaatcatcc ggtcattacc gggcatgaat gtgccggggt tattgtcgaa
     7441 gtgggtaaaa atctcactgg caaatataaa aaaggccagc gttttgtatt gcaaccggcg
     7501 atggggttac caagcggata ttcagcaggc tacagctacg aatattttgg cggtaacgcc
     7561 acctatatga ttattcccga aatcgccatt aatttgggct gcgtattacc gtatcacggc
     7621 tcttattttg ctgcggcgtc gctggcagag cctatgtgct gcattattgg tgcttatcat
     7681 gccaattatc acaccacgca atatgtttat gagcatcgca tgggcgtcaa acctggcggc
     7741 aatattgcac tgctggcgtg tgcgggtccg atgggcattg gcgctatcga ttacgccatt
     7801 aacggtggca tacaaccgtc acgggtggtg gtggtcgata tcgacgacaa acgcctggcg
     7861 caggtgcaga agctgctgcc ggtggatctg gcggccagta aaggcattga gctggtgtat
     7921 gtgaatacca aagggatgag cgatccggtc cagacgctgc gggcgctgac aggagatgtc
     7981 gggttcgatg acatttttgt ttatgcggcg gtgcctgctg tcgttgagat ggctgacgaa
     8041 ttactggcgg aagatggctg tctgaacttc tttgccgggc cgacggataa aaacttcaaa
     8101 gtgccgttta atttctacaa cgtccattac aacagcacgc acgtagtcgg tacatccggc
     8161 ggttcaacgg acgacatgaa agaggcgatt gcgttaagcg ccacggggca gttgcagccg
     8221 tcctttatgg tcacgcatat cggtgggctg gatgcggtgc cagataccgt gctcaatctg
     8281 ccggatatcc ctggcggtaa aaaactcatt tataacggcg tgaccatgcc gctcactgcc
     8341 attgccgatt ttgccgaaaa aggcaaaacc gatccgctgt ttaaagagtt ggcgcggctg
     8401 gttgaggaaa cgcacggcat ctggaatgaa caggccgaga aatatctgct ggcacaattt
     8461 ggcgttgata tcggggaggc cgcgcaatga tgtccctggc gtggccatta tttcgcgtta
     8521 cggaacaggc agcgttggct gcctggccgc aaaccggatg tggcgacaaa aataaaattg
     8581 atggcctggc ggtcaccgcg atgcgccagg cattaaacga cgtcgctttt cgtggacgag
     8641 tggttatcgg cgaaggagaa attgaccatg cgccgatgct gtggattggc gaagaggtgg
     8701 gcaaaggcga tgggccagaa gtcgatatcg cggttgaccc gattgaaggc acgcggatgg
     8761 tagcgatggg gcagagcaat gcgctggcgg tcatggcttt cgcccctcga gatagcctgt
     8821 tacatgcgcc cgatatgtat atgaaaaagc tggttgttaa tcgactggct gccggggcga
     8881 ttgatctgtc tttgcctctg gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac
     8941 cgctggacaa gctgcgcatg gttacgctgg ataaaccgcg cctgagtgcc gcgattgaag
     9001 aagcaacaca gctgggcgtg aaggttttcg ctctgccgga tggcgatgtc gccgccagcg
     9061 tgctgacctg ctggcaggat aatccttacg acgtgatgta caccatcggc ggtgcgccag
     9121 aaggcgtgat ttctgcctgt gccgttaaag cattaggtgg ggatatgcag gccgagctga
     9181 ttgacttttg tcaggctaaa ggggattaca cggaaaatcg gcagattgca gagcaggagc
     9241 gcaagcgttg taaggcaatg ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg
     9301 tgagggggaa cgatatcctc tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg
     9361 gtatccaaca gacggcgaat ggggtgcgga cgcagacatt actgatcggc ggcgcggacc
     9421 aaacgtgtaa tataatagac tccctgcatt gatggtgatc tatcggaaga ccaatggcga
     9481 cgctgacaga agatgatgtg cttgagcaac tggatgcaca ggacaattta ttttcattta
     9541 tgaaaactgc gcattctatt ttgctccagg ggatacgcca gtttctgccg tcgctgtttg
     9601 tcgataacga tgaagagatc gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc
     9661 cgcttgacga tattgatgtt gcgctgcgtt tgatttatgc gctggggaaa atggataaat
     9721 ggctgtatgc cgatatcacg cacttttccc agtactggca ttacctgaac gaacaggatg
     9781 aaacgcccgg atttgccgat gacatgacct gggattttat cagcaacgtc aatagcataa
     9841 cctgcaatgc gacgctctat gacgcgttaa aagcgatgaa gttcgccgat ttcgccgtct
     9901 ggtcagaggc gcgttttagc ggaatggtca aaacggcgct gacgctggca gtaacgacca
     9961 ccttaaagga attaacgccg tgaaaattga attaacggtg aatgggctga acgtgcaagc
    10021 gcagtaccat gatgaagaga tagaacgcgt gcataaaccg ttgctgcgta tgctggcggc
    10081 cttacagacg gtaagtccgc agcgacgtac ggtggttttt ctttgcgctc cgccggggac
    10141 gggtaaatct accttaacta ccttctggga atatctcgcg caacaagacc ctgaattacc
    10201 cgctatccag acgctcccga tggatggttt tcaccattac aatagctggc tggatgcgca
    10261 tcaattgcgc cccttcaaag gcgcaccaga gacatttgac gttgcgaaac tggcggaaaa
    10321 tctgtgccag gtagtggaag gggattgtac gtggccgcag tatgatcgac aaaagcatga
    10381 tcctgttgaa gatgcgttgc acgttaccgc accactcgtc atcgtcgaag gaaactggtt
    10441 gttgctggac gacgagaagt ggtgccagct tgcgcaattt tgtgatttct cgatctttat
    10501 caacgcgcca gctactgcgt tgcgggaacg actggtaggg cgcaagctgg cgggaggggt
    10561 atcgctggca gacgcagaag ctttttatga ccgtaccgac gggccgaatg ttcgccgggg
    10621 gctggaagag agtctgcctg caaatttgac cttaatgatg accgctacgg gtgaatatcg
    10681 tctggtggat taacgttcct gattagacca tcttccagtc gcaccacacg agagaaatga
    10741 cgccgggtaa atgcggcgtc atgactgatt gctacgacgc tcgtgcctcg ctggccgcac
    10801 ttctccagcc agctttcaaa cacgctcaac caattttcgt caaaatcccg actcggctcg
    10861 tccagcagca atagtggcgg agagagtgct tccaggcagg cgacggcgac catccgtcgc
    10921 tgcgcactat gcagatccaa cgggtgcgag ttggcagtgt ctgctaactg acaacattgc
    10981 agggcagcat gggtacgttg agtgatttca tccgccggga atttttgcag tttcaaacca
    11041 aatgcgatct cgtcagcgac ggtgctgtga aaaagttggt tttccgcttc ctgaaacagt
    11101 acgccaacct ttgccgcgcg ctggcggttt ttcaggtttt tcatcgcctg ttgttgcagc
    11161 ataaccgtac cggctgtggg cgtaagaaga cctgccatta ctcgtaacag cgtcgatttt
    11221 cccgcgccat tgtcgccggt cagcgccaac cattcacctt gtttgagttg cagagaaatg
    11281 tcgcaaagac aatccgttgc cgcgccaggc cagcgataag atatcttatt taacgttaac
    11341 atagtggctg gagcgctccg ttttgcaatt gccacgcttg ctggcagtag ttcaggaaag
    11401 gtgtgcgatg acgttcaaaa agaataatca gcgaaccacg ttcaaacgcc cagtgttgta
    11461 agcgctgcaa cagcatttcg ctggcctgcg gagtcaggcg gctaaaggct tcatcaagaa
    11521 ttaagagctt tggctgcatc gcgatggcgc aggcgatgac cacgcgctgt gtctctccac
    11581 cggagagtgt tgccgggtga cgatggcgca acggctggca ctcggtcaga gtcagggcgg
    11641 catcaatccg cgccataatc tctgcttcag caagacacag attttctggc ccaaaggcaa
    11701 cttcttcttc cacgctaaag gtacagccgg agagctgcaa atagggcgat tgctggacga
    11761 gctggatggt ggcggattgt tcattaagcg ggagatgccc aatgggcgta ccgaggagcg
    11821 tccccgtgcc tgttatctct cctggcagaa agtccggata ccagcccgcc atcagctgtg
    11881 ccagcgtact tttgccactg ccgttgtcac caaagatggc gaccatcccc ggtttgacat
    11941 agtgaaagtc ataacagaac ggaggatgcg ttgagtgtgt cgggcaataa cggaactgtt
    12001 ctaacgtaac catatccacg ctccgaattc tgtcagcatc agcaagatca tggtgtaacg
    12061 cgctacgcgt tgtagggtgc tatcggctgg tgcccacaac gttgttcgtc ggttgtggat
    12121 ccgaaatgcc cgcatatcca gcgccgcacc acggacagcc agatcgttaa gcgcattgtg
    12181 tgttagcgga ataattaacg cgggcatcgc acgtaaacgc tgataccagc cctcatccag
    12241 cggcacgccg cgggcacgct gcgcttcatg aataatcgct aattgccgtt ttaactgctc
    12301 aacgaccaat aacggcccgg caaacagata agcgacgcct ggcggcaggc gagaagcaaa
    12361 cagagcgcga ataaaacatt gcacggggac gtactgcatc cacaactgtg aggttgaaac
    12421 aatcgccaga atccgcaacc agagcgtaat agcatgcgac caccgttctg gcgagcgtgg
    12481 cgtgccgctt agccattctg gtagccagcc accgtgtacc agccacagcc cggctcccag
    12541 cgagaacatc agccagacga catattttgc ccgccgccgc gtggccttta gcgcaataag
    12601 gcagaaaaag gtcgccgcgc tatagatcgg caaaatggtc tgcgcgggca ggataagcgt
    12661 ggtgcaggcc gccaatgccc acagtgttaa tgaggtaaat ggatgcattt agcgcacggc
    12721 agccattgcc ggaaaatggc gcgtggtgcg aatcggcagc tggcgcagta aaagccagac
    12781 aatcaccgct gtaagaattt tatcgaccag gttcgcgcct atcaccgtaa tcgccaccga
    12841 ctccaccagg ttttgcccca tcgagtgcat ccaggcaacg aacaaatcgg ctccgctacc
    12901 ggtgacaccg ccgaacagcg cagtacgcag cggtacagcg acgaccgtta ccgcaagcgt
    12961 aataatcacg ccgctgacaa cgacttttgg cagggtgcga aaccaacccg ctcgcgccag
    13021 gcaaccggcg accaggccga tgaccatcgc gacgggtgca aatgcggcgg cgatcgggtt
    13081 ggtcagcaat ccccagagta aattggtcag taagccggtc agcattccga tcaccggacc
    13141 aagcagtacc gcgctaataa gtgtgccgat ggagtcgaga aatataggta atttgaccat
    13201 gctggccagt tgcccgccga tcatattgat ggcgatagat ataacgatca acaccagtgc
    13261 ctgactggaa aagtggctac gcgccataag aaaacctctt tgaaagtagt tatttaacgg
    13321 gggaaatatg caactcgtcg taaccagaaa gcgcgcaagg ttggcggcta aacgccagtt
    13381 cctcaaggct accgatcacc agttcaagcg tggtccgcac ggtacagccg ggcatcatat
    13441 gaccgccaag catcgtgccg tgtggatcgg aaacgcagag atgaagatgc tcaccactct
    13501 gttccagcgt gccgtttagt gagatcactt cgaatgtacc gttcaggagc gtggtgcctt
    13561 cttgcccggc atagcgcaag gcgacgtcag tcaggctacc ggtacagcct gcaatccagg
    13621 cggcatgaag ttgatgctgc tgtgcaaaag cgtgtagctg ggaaaaaact tcctgcccgg
    13681 ggaggaggcg cagtgcgtaa aagcgtgcgg aggaagaata tgggtagggc aatgtcatca
    13741 ttggagtact ccgaaactcg gacgtattgc ggataatatc gacgctgcat cagtcatatt
    13801 ctgatataag tcagggtttg tgacggcaca acgaaaagat tttgctacgc ttattcgtga
    13861 ttctgcaaag tggaataaat tccacagggg cggggctgaa aacgtgacgt aagtcagata
    13921 attgctttct ttgactggac aatcattcct tttattccac gtttcgctta tcctagctga
    13981 agcgtttcag tcgattaaat gttcgacaat taaccaatca gtcgcagttt gcgacaggta
    14041 aggtttcccc ggacgatttg ctggattact ctgtctggca aatttgctca gggaaaacct
    14101 tgcaggagat ctatgaccgt acgcgtagcg ataaatggct tcggtcgcat cgggcgtaat
    14161 gtggttcgtg ctttgtatga atccggacgc cgggcggaaa ttaccgtggt ggcaatcaac
    14221 gaactggcgg atgctgcggg catggcgcat ttgttgaaat atgacaccag ccatggccgt
    14281 tttgcatggg aagtacgaca ggaacgcgat caactttttg ttggtgatga cgccatccgc
    14341 gtattgcatg aacgttcact gcaatcgctc ccctggcgtg aacttggcgt tgatgtagtc
    14401 ctcgactgca ccggcgtata tggctcccgc gagcatggcg aagcgcatat tgccgccggg
    14461 gccaaaaaag tgctcttttc acatcctggc agtaacgatc tcgacgcgac cgttgtttac
    14521 ggcgtcaatc aggatcaact tcgtgcggaa caccgcatcg tttctaacgc ttcctgtacc
    14581 acgaattgca taattcccgt catcaaattg ttagatgatg cgtacggtat tgagtccggc
    14641 actgtgacca caattcactc cgccatgcac gatcaacagg ttattgatgc ataccatcct
    14701 gacctgcgtc gcacccgggc agccagccag tcgatcattc cggtcgatac taaactggcc
    14761 gccggtatca cacgattttt tccgcaattt aacgatcgct ttgaagcgat tgcggtacgt
    14821 gtgccaacca taaatgtgac ggcaatcgat ttaagcgtga cggtgaagaa acctgtaaaa
    14881 gccaatgaag tcaacctgtt gctgcaaaaa gcagcacaag gtgcatttca tggtatagtt
    14941 gactatacgg aattgccgtt ggtctctgta gattttaacc acgatccgca cagtgccatt
    15001 gtcgatggca cccaaacccg ggtcagtggc gcacacctga tcaaaacgtt ggtctggtgc
    15061 gataacgaat ggggctttgc taaccgaatg ctcgacacga cgttagctat ggctactgtt
    15121 gctttcaggt aagacgcaag cagcgtctgc aaaactttta gaatcaacga gaggattcac
    15181 catgtctgta attaagatga ccgatctgga tcttgctggg aaacgtgtat ttatccgtgc
    15241 ggatctgaac gtaccagtaa aagacgggaa agtaaccagc gacgcgcgta tccgtgcttc
    15301 tctgccgacc atcgaactgg ccctgaaaca aggcgcaaaa gtgatggtaa cttcccacct
    15361 gggtcgtcct accgaaggcg agtacaacga agaattctct ctgctgccgg ttgttaacta
    15421 cctgaaagac aaactgtcta acccggttcg cctggttaaa gattacctcg acggcgttga
    15481 cgttgctgaa ggtgaactgg ttgttctgga aaacgttcgc ttcaacaaag gcgagaagaa
    15541 agacgacgaa accctgtcca aaaaatacgc tgcactgtgt gacgtgttcg taatggacgc
    15601 attcggtact gctcaccgcg cgcaggcttc tactcacggt atcggtaaat tcgctgacgt
    15661 tgcgtgcgca ggcccgctgc tggcagctga actggacgcg ctgggtaaag cactgaaaga
    15721 acctgctcgc ccgatggtgg ctatcgttgg tggttctaaa gtatctacca aactgaccgt
    15781 tctggactcc ctgtctaaaa tcgctgacca gctgattgtt ggtggtggta tcgctaacac
    15841 ctttatcgcg gcacaaggcc acgatgtggg taaatccctg tacgaagctg acctggttga
    15901 cgaagctaaa cgtctgctga ccacctgcaa catcccggtt ccgtctgatg ttcgcgtagc
    15961 aaccgagttc tctgaaactg caccggctac cctgaaatct gttaacgatg tgaaagctga
    16021 cgagcagatc ctggatatcg gtgatgcttc cgctcaggaa ctggctgaaa tcctgaagaa
    16081 tgcgaaaacc attctgtgga acggtccggt tggcgtgttc gaattcccga acttccgcaa
    16141 aggtactgaa atcgtggcta acgctatcgc agacagcgaa gcgttctcca tcgctggcgg
    16201 cggcgacact ctggcagcaa tcgacctgtt cggcattgct gacaaaatct cctacatctc
    16261 cactggcggc ggcgcattcc tcgaattcgt ggaaggtaaa gtactgcctg cagtagcgat
    16321 gctcgaagag cgcgctaaga agtaaaaaat cacagggcag ggaaacctgc ccttgtttca
    16381 gcgcgctttt agagcacgca ttttttcata ctctaaataa ttcgagttgc aggaaggcga
    16441 caagagagtg aatccccagg agcttacata agtaagtgac tggggtgagc gaacgcagac
    16501 gcagcacatg caacttgaag tatgatgagt ataaggcccg acgatacagg acaagagaca
    16561 tgtctaagat ttttgatttc gtaaaacctg gcgtaatcac tggtgatgac gtacagaaag
    16621 ttttccaggt agcaaaagaa aacaacttcg cactgccagc agtaaactgc gtcggtactg
    16681 actccatcaa cgccgtactg gaaactgctg ctaaagttaa agcgccggtt atcgttcagt
    16741 tctccaacgg tggtgcttcc tttatcgctg gtaaaggcgt gaaatctgac gttccgcagg
    16801 gtgctgctat cctgggcgcg atctctggtg cgcatcacgt tcaccagatg gctgaacatt
    16861 atggtgttcc ggttatcctg cacactgacc actgcgcgaa gaaactgctg ccgtggatcg
    16921 acggtctgct ggacgcgggt gaaaaacact tcgcagctac cggtaagccg ctgttctctt
    16981 ctcacatgat cgacctgtct gaagaatctc tgcaagagaa catcgaaatc tgctctaaat
    17041 acctggagcg tatgtccaaa atcggcatga ctctggaaat cgaactgggt tgcaccggtg
    17101 gtgaagaaga cggcgtggac aacagccaca tggacgcttc tgcactgtac acccagccgg
    17161 aagacgttga ttacgcatac accgaactga gcaaaatcag cccgcgtttc accatcgcag
    17221 cgtccttcgg taacgtacac ggtgtttaca agccgggtaa cgtggttctg actccgacca
    17281 tcctgcgtga ttctcaggaa tatgtttcca agaaacacaa cctgccgcac aacagcctga
    17341 acttcgtatt ccacggtggt tccggttcta ctgctcagga aatcaaagac tccgtaagct
    17401 acggcgtagt aaaaatgaac atcgataccg atacccaatg ggcaacctgg gaaggcgttc
    17461 tgaactacta caaagcgaac gaagcttatc tgcagggtca gctgggtaac ccgaaaggcg
    17521 aagatcagcc gaacaagaaa tactacgatc cgcgcgtatg gctgcgtgcc ggtcagactt
    17581 cgatgatcgc tcgtctggaa aaagcattcc aggaactgaa cgcgatcgac gttctgtaag
    17641 atattccttt ctgcttatct caaggcccgc tctgcgggtc tttttttcgc caaaagcaaa
    17701 attgcctgat gcgctacgct tatcaggcct acacgtatcc tgcaatatat tgaatttgca
    17761 ggtttttgta ggccggataa ggcgttcacg ccgcatccgg catgacaaag cgcacattga
    17821 tatctgtctg agccccgctc tgcggggatt ttttcgccaa aagcaaggtg attcagatga
    17881 gaaatctgtg atctatttgg caaaattatg ctttattgtt tacccttgtc agactgcccg
    17941 tcataaggcg gcggagtgta tttctccatt ttgagtcagt tgaaaaggaa tattgaatgg
    18001 aagatttgaa tgttgtcgat agcataaacg gcgcgggaag ctggctggta gctaaccagg
    18061 cgctgctgct aagttatgca gtaaacatcg tggcggcact cgcgatcatc atcgttggtt
    18121 tgattatcgc gcggatgatt tccaacgcgg tgaatcgcct gatgatctcc cgtaaaatcg
    18181 atgccactgt tgctgatttt ctttctgcat tagtccgtta cggtattatc gcctttacgc
    18241 taatcgctgc cctgggacgc gtgggtgtac aaacagcgtc agtcattgct gtactcggtg
    18301 ccgcaggctt agctgttggt ctggctttgc aggggtcact ttctaacctg gccgctggcg
    18361 tgttacttgt catgttccgc ccgttccgtg ccggagaata tgttgacctc ggcggcgtag
    18421 ccggtactgt gctgagtgtg cagattttct ccactaccat gcgtactgca gacggtaaaa
    18481 ttatcgttat tccgaacggt aaaattattg ccggaaatat tattaacttc tcccgcgagc
    18541 cagttcgccg taacgaattt attattggcg tggcgtatga ttccgatatc gatcaggtta
    18601 agcagatcct gaccaatatt atccagtctg aagatcgcat tttgaaagat cgcgaaatga
    18661 ctgtgcgcct gaacgaactt ggtgcatcgt cgattaattt cgtggtccgc gtctggagca
    18721 acagcggcga tctgcaaaac gtgtactggg atgtgctgga gcgtattaaa cgtgaatttg
    18781 atgccgccgg tatcagcttc ccgtacccgc aaatggatgt gaactttaag cgggtgaaag
    18841 aagacaaagc tgcgtaatca acgctgaggc cagataatac tctctggcct ctctcttatt
    18901 agttattctg attgctaatt aatattatca atttccgcta ataacaatcc cgcgatatag
    18961 tctctgcatc agatacttaa ttcggaatat ccaacgtgtt ttcttattac tttcaaggtc
    19021 ttgctcttgg ggcagctatg attctgccgc tcggcccaca aaatgctttt gtgatgaatc
    19081 agggcattcg tcgtcagtac cacattatga ttgccttact ttgcgctatc agcgatttgg
    19141 tcctgatttg cgcggggatt tttggtggta gcgcgttatt gatgcagtcg ccgtggttgc
    19201 tggcgctggt cacctggggc ggcgtagtct tcttgctgtg gtatggtttt ggcgctttta
    19261 aaacagcgat gagtagtaat attgagttag ctagtgccga agtcctgaag caaggcagat
    19321 ggaaaattat cgccaccatg ttggcagtga cctggctgaa tccgcatgtt tacctggata
    19381 cttttgttgt actgggcagc cttggcgggc agcttgacgt cgaaccaaaa cgttggtttg
    19441 cgctcgggac aattagcgcc tctttcctgt ggttctttgg gctggctatt ctcgcagcct
    19501 ggctggcacc gcgcctgcgc acggcaaaat cacagcgcat tatcaatctg gtagtgggat
    19561 gtgtcatgtg gtttatagcc ttgcagctgg cgagagacgg tattgctcat gcacaagcct
    19621 tgttcagtta ggcgctatct gatggaaaaa taaaacagag gcgctaagct tgcctccaga
    19681 ggtcctgaat tttgggcaag tcaattaaca cggagagact aacgtgaagt tcaaagttat
    19741 cgccctggcg gcattaatgg gtattagcgg gatggcagcg caggctaacg aattgccgga
    19801 tggaccgcat attgtcacct ccggtacggc aagtgtggat gcggtgccag acattgccac
    19861 tcttgcgatt gaagttaacg tggccgcgaa ggatgccgct acagccaaga aacaggcaga
    19921 tgagcgcgtc gcacaataca tttccttcct tgaactcaat cagatcgcga aaaaagatat
    19981 cagctcagcg aatttacgca cccagccaga ttatgattat caggatggta aaagtatcct
    20041 gaaaggctac cgcgctgtga gaacggtgga agtcacgctc cgtcagttag acaaactgaa
    20101 ttccttgctg gatggcgcgc tgaaggcggg tcttaacgaa attcgttctg tgtcgctggg
    20161 cgtggcgcag ccggatgcct ataaagacaa agcgcgtaag gcagcgattg ataacgcgat
    20221 tcatcaggcg caggaactgg cgaacggctt tcatcgtaaa ctggggccgg tatatagcgt
    20281 gcgctaccat gtttccaact atcagcccag cccaatggtg cggatgatga aagccgatgc
    20341 cgcgccggtg tctgcccagg aaacttacga gcaggccgct attcagtttg atgatcaggt
    20401 cgatgtggtc ttccagttag aacctgtgga tcaacaaccc gctaaaacac ctgcagcaca
    20461 ataatacttg tagcccgacg tattcgtcgg gctaatgcct ttacccgata ttgcctcctt
    20521 tttctgtaat tttctgtaaa accatgacgt aatcacctat ctttgctcgc ggtttcatca
    20581 aggataggct gactgaaacg gttaagcgga caaggatgcg gggagggggg atggacattt
    20641 ttatttcgaa aaagatgcgc aatttcattt tattagcgca aaccaataat attgcccggg
    20701 cggcagaaaa aatccatatg acggcttcgc catttggtaa aagcattgcc gcgctggaag
    20761 aacaaattgg ctatacgcta tttacccgca aagataataa tatcagcctc aataaagccg
    20821 gtcaggaact gtatcaaaaa ctgttcccgg tttatcaacg actttctgcg attgataatg
    20881 aaatccataa ctcagggcgt cgttcacgag aaattgtgat tggcatagat aatacttatc
    20941 cgacaattat tttcgatcag ttgattagtc tcggagataa gtatgaaggc gtgaccgccc
    21001 agccagttga gttcagcgaa aacggtgtta tcgataatct ttttgaccgt cagttagatt
    21061 ttattatctc cccacagcat gtatctgcac gagttcagga actggaaaac ctgactatca
    21121 gtgaattacc accgctgcga ttgggctttc tcgtttctcg ccgttatgaa gagcgtcaag
    21181 agcaagagct gcttcaggag ctgccctggc tacaaatgcg ctttcaaaac cgggctaact
    21241 tcgaagcaat gattgatgcg aatatgcgac cttgtgggat caatcccacc attatttatc
    21301 gtccctacag ttttatggcc aaaatcagcg ccgtggagcg cggacatttt ctgacggtga
    21361 tcccgcattt tgcctggcga ctggtgaatc cggcaacgct gaaatatttt gatgcgccgc
    21421 acaggccgat gtatatgcag gaatatcttt attcaatcag aaatcatcgt tataccgcca
    21481 cgatgcttca gcatattgct gaagatcgtg acgggacaaa ccattaaccc agcatcgagc
    21541 cggttgcaat taaattacgg tgtaagtcga agacgtggct aagatcgtgg tgaatatgtc
    21601 cgccgggcgc attttccaga tagcgatgca gataatcccg atacatagga tgtgcacaat
    21661 tatcaatgat agtgcgggcg cgttgaagcg gggaaagacc gcgcagatcg gcgatccctt
    21721 gttcggtgat gatcactttg acgctgtgtt cgctgtgatc aacatggctg cacattggca
    21781 cgacggttga gatcttgcct tctttagcaa tcgacggagc cataaagatc gacagatacg
    21841 cgttgcgttc aaaatcaccg ctgccgccga tgccgttcat cagatcgacc ccggctacgt
    21901 gcgttgagtt ggcatgcccg taaatatcaa actccaggcc gacgttcaga gcgatgacgc
    21961 ccagacgacg gatgatttcc gggttattgg aaatctcctg cggacgcaac acaatgcggc
    22021 tggcaaagta atccatattg tcgtaaatct tgcgcaggga atcggccgag attgtcaggc
    22081 tggaggcgct ggccccgctg attttgccgg tttccagtaa atgcaccacc gattcctgta
    22141 gcacttccga atacatcatg aacggaggaa tttccgggtt ttcccccaga cgcgccatta
    22201 ccgcattatt gatattgccc acgccacttt gcagcggcag aaattccggc ggaatacgcc
    22261 catgcgccat ttcctgcaat aagaacgtga ccacgttatc ggcaatctgc tggcacatgg
    22321 gattttgctt atccagcata ttaccggcgt cgggcaagtt ggtttccacg acggcgacaa
    22381 tctttttcgg atcgatttgc acatagcggg taccgacgcg atccattgca tggaagatcg
    22441 acacgctatt gcgccgcggt ggcgcgccag gaatcacaat atctgccagt tctgcaacgc
    22501 gcggatcgtg atagtgatta agttcaatga tcactttctt cgcccgcagc agccaggtcg
    22561 gcgcattacc gatcccgctg gttaaccaga ctcgaccatc cggtgccagt gccgatgctt
    22621 caatgacggc aacatcaatt tcgccgaaga aaccgtaatt gaccatttgc gccacttcgc
    22681 tcaaatgcag gtcaacgaaa ctcaccgcgc cctgattgat ctttttacgt aaaccggacg
    22741 atgtttgata tggtgcacgc caggaaacag catcggcgtc agaaagtaca tcgtcagcgg
    22801 cggcgctgat tgacgccccc gtcagcaggc gaatttgata cggctttttg gcctcatgct
    22861 gttcgttagc tctgcgggca atcgcggtag gtagggcttt cggcgaaccc gctggggtaa
    22921 agccgctaaa tgccaccatg tcgttatgct ggataatttc tgccgcttca tcggcggtca
    22981 tccttgtcca ctgagtttcc atttttactc cttcgttcgc agggattaat gaccaacgaa
    23041 attaggttta cgtttttcga ggaaagcgtt catcccttcc tggtaatctt cgctgtcata
    23101 aaccgcgcgg cgcatcccct gaatacgttc aaattcatcg gagttcatgg tgtgtgcttc
    23161 gcccagtaca cgcagctctt ctttgataac ggcaatggct aacggcgctt tctcagagat
    23221 gtggtgcgcc atttgtaagg tgaaatcttc cagttcttcc acttccacaa catggttgag
    23281 gatgccgaca gccagcgcgc gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt
    23341 gacaatgtgg aagcccgcgt cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac
    23401 gccgaggttt acaggcgtca ttgagaaggt tgaggtactg gcggcgataa tcagatcgga
    23461 actcatgatc atttcaaatg cgccacccca aacactacct tccaccatcg aaataatcgg
    23521 tttcgggaat ttttggatca tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg
    23581 atcgcgaccg ccagacggca gttcgtgaat atcgtgacct gcggagaaga ctttggatcc
    23641 actcggcgcg cgcaaaatga tacagcgaat ttccggccgg ttgagatcgc ttaacgcctg
    23701 cataagatca tcaataaaga ctttacttaa ggcattaagt tttcggccat agttaaactc
    23761 aatgaccgcc actttgttga tagtggcaac gttcacatac tgataagaca taaaaattcc
    23821 tttaatcaaa atattgcgtc tggataaatt cactgagctg ccgcaggccg gtgcgcggtg
    23881 agagcgtatt gtttttgacc gctaaaagcg tctggcggta atagcgatcg aaatcttcat
    23941 tcgcgaacag gtgattcagt acttcttctt cggtctgctt acgcagccat tccaccgatt
    24001 gttgttgccg cacttgttgt aaacgaccac tggcagttag cgcggttttg aagtcgatga
    24061 tggcgtgcca gatctcatcg attccacgtt tttccagtgc gctacaagtc agaacccgtg
    24121 gctgccattc gtcgtattta cgtcgcagaa tatgcagggc actctcgtac atatgccggg
    24181 caatggcgac attggtatgg ttatcgccat cgtctttgtt gataacgatc agatcagcca
    24241 cttccatcag ccctttttta atgccctgca gatcatcgcc gccaccggca atttgcaacg
    24301 agataaaaca gtccaccatg cgggcgactt ctgtttccga ctgcccgacg ccaaccgttt
    24361 cgacaatcac tacgtcataa cccgctgctt cgcataacag cattaattcc cgcgctcgct
    24421 gactggcacc gcccagatga ccggaggatg gtaccgggcg aataaacgcc gcttcggcac
    24481 gcgccaggtc attcatgcgg gttttatccc cgagaatgct accgccagtg accgggctgc
    24541 tgggatcgac cgcaataacc gcgaccttta atccctctcg aatcaacaac atgccaaagg
    24601 cctcaagaaa ggtacttttc cccgcgccgg gggtgccggt aacgcccagt cgcagggtgt
    24661 taccgcagta cggcataatg gcatcaagca gctgcgtact tagtgcctga tgacgcgggt
    24721 gacggctttc caccagcgtc atggcctggg cgagtgtggc acgctcaccc tgacgtaagc
    24781 ggcgaatact ttctgccagc gtggcttcat taatcatgat gctggcttat cagattcagt
    24841 acgtcgcgca cactgtcgag cataggtgta cctggaccat aaatcgccgc cacgccgcgc
    24901 tcttgcagga aggcgtaatc ctgcggcgga atgacgccac ccgcgaccac gcagatatct
    24961 tcgcgtcccc attttttcag cgcttcgacc agttccggga tcagcgtttt atgaccggca
    25021 gccagtgagg atgcgcccac tacgtgaacg tcgttttcta cggccaggcg ggcgatctct
    25081 tcaggtgtag agaacatcgg gcttaaatct acgtcgaaac cgagatcgga ataggcgctg
    25141 gcgatcactt tcgcgccgcg atcgtgtcca tcctggccca tcttagcgat cagaatgcgc
    25201 gggcgacgac cattgtcggc aaggaactgc tccgtttgcg caacaatggc atcgaactcg
    25261 gaggccgatt tctcagactg atgatagctt tgcgcaatca cgccggtaac acactggctt
    25321 ggcaccagat aacggtcgaa agcgacttcc agcgcatcgg aaatttcacc cagggtggca
    25381 cgaacgcgag cggcattaac agcggcagcc agcaggtttt cgttatgctg tgcggcgtga
    25441 gtcagggcgt tcaacgcggc ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc
    25501 agcgaagcaa tttgctcgtt acgcaccatc acgttgtcga tctcaagtac atcggtttcg
    25561 tcttcgtgat ccagtttgta cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc
    25621 agcgactgtt cgcgcgctga ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc
    25681 gctttcgcca tgccaccggc ttcgtcgatc tgttggataa tagctctggc ttgtttgacg
    25741 atttgatcgg tcagcgactc aatgtaatag gatccggcca gtggatcgac ggtgcggcag
    25801 agttctgatt cttcctggat gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg
    25861 gtaggcaaac caagcgcttc gtcaaaggcg ttggtatgca gtgactgagt accgcccagc
    25921 gtcgcagcca gtgcttcaat ggttgttata cggatcctgt tcagtcaggc tccagcctga
    25981 ggtctggcag tgggtacgca gcgccagtga tttcggatcc tgtgcgccaa atccactgac
    26041 cgcttcgctc cataaataac gtgccgcacg caacatggcg acgttcataa acagatccat
    26101 gccgataccg aagaagaacg acaggcgagg agcgaagtca tcaattttca gtccggcaga
    26161 gattgctgct ttgatgtact caatcccatc agcgagcgta aatgctacct gctgcacgca
    26221 gttggcaccc gcttccccca tgtggtaacc gctgatactg atggtattaa atcgcggcat
    26281 gttgccggaa caccaggcga tgatgtcggc gataatgcgc attgacggtt ttggtgggta
    26341 aatataggtg ttgcggcaga ggtactcttt gaggatatcg ttctgaatgg tgccggtcag
    26401 tttatcaggt gtaacacctt gctcttctgc ggcgacgata taaaacgcca gtactggtag
    26461 cactgcgcca ttcatggtca tcgaaaccga cattttatcc agcgggatct ggtcgaacag
    26521 gactttcata tcttccacgg tgtcgatagc gacgcccgct ttgccgacgt cgcccgccac
    26581 gcgcgggtta tcggagtcgt agccacggtg ggtggcaagg tcaaacgcaa cggaaagacc
    26641 tttttgcccg gcggccaggt tacggcgata aaaagcgttg gactcttttg ctgttgaaaa
    26701 accagcatac tgacggatgg tccacggttg ggcggtatac atagtggcac gcgggccacg
    26761 aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc agattatcga gatcggcttc
    26821 ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc tgaaccagcg agtcgacagt
    26881 tttctcccga cggctcaatt ccttgttggc aagctgttgc cactcctgca cgttagacat
    26941 tatttgctcc gtaaaacgca atcaaaatga gggtatttgc gcctacggtg agctttttac
    27001 gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg ttttttgccg cctgagattt
    27061 gtctattttg tgatgtgatt caaggaatac aaaaagaagt gaggacaacg cctgatatgt
    27121 gctatcaggc attatttgat ggattaatcc tgacgaagga ctttgtgacc ataatcgagt
    27181 aacgcatcag tgactttacg catcatgcgg ctttcaggag caaagcggtg ccagtagagc
    27241 atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt caccgctggc cagctctttc
    27301 tcgatttgca ggtgcgggat catacagcag gtggtgccct ggcgagcaag ttgtacgaac
    27361 gcttctgaag aattaacgat atggcagggc acgctgcctg gaggcagatc gaagttttgc
    27421 tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg cgaccactgg cgctttcagt
    27481 aatgccgaac gcgttacgcc gttagggaaa tatttttcgg caaagggttt tgagctgacg
    27541 aacagatagt cgagcgcacc aagtttatcg acaagacaac tcggcagcgc ctgatgttga
    27601 atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt cctgagtgcg ggtttcatct
    27661 tctacctgca agttgaggcg gataggcgaa tcagccaaca caggagccag tgcaggaagc
    27721 aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa gcagcagcgg agtcgaaccg
    27781 gtttgttcat cgcccagcca ctcttcttcc agcaactcca cctggcgcag cagtgccagc
    27841 agtttttgcc cttgttccgt cgggcgcggc ggtacggtac gcaccaacag cggctgcccg
    27901 aacatatttt ccagttgctt aatgcgctgt gagacggctg attgtgtaat gcacagcttt
    27961 tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat ccagtgcctg taatgttctg
    28021 tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa tgctttttcc tgcactatga
    28081 cacaattttg tgtcaggttg catataccga agccgtcggc gggtaacatt tgtattcgtg
    28141 gttaaacgcg cagcttaaaa gatcagggat ttgcgctaat gcatttcgct cagcgagttc
    28201 gcgccctggt tgtgttgaat ggcgtggcgt tattgcctct gttcgcaccg tggagcggca
    28261 tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg gatgcccgcc attgcgcgat
    28321 attttatgga tgagttaacc acgcggcttg ccaacggggt ctgaatcgct ttttttgtat
    28381 ataatgcgtg tgaaatttca taccacaggc gaaacgatca tgacgcagga tgaattgaaa
    28441 aaagcagtag gatgggcggc acttcagtat gttcagcccg gcaccattgt tggtgtaggt
    28501 acaggttcca ccgccgcaca ctttattgac gcgctcggta caatgaaagg ccagattgaa
    28561 ggggccgttt ccagttcaga tgcttccact gaaaaactga aaagcctcgg cattcacgtt
    28621 tttgatctca acgaagtcga cagccttggc atctacgttg atggcgcaga tgaaatcaac
    28681 ggccacatgc aaatgatcaa aggcggcggc gcggcgctga cccgtgaaaa aatcattgct
    28741 tcggttgcag aaaaatttat ctgtattgca gacgcttcca agcaggttga tattctgggt
    28801 aaattcccgc tgccagtaga agttatcccg atggcacgta gtgcagtggc gcgccagctg
    28861 gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg tgaccgataa tggcaacgtg
    28921 atcctcgacg tccacggcat ggaaatcctt gacccgatag cgatggaaaa cgccataaat
    28981 gcgattcctg gcgtggtgac tgttggcttg tttgctaacc gtggcgcgga cgttgcgctg
    29041 attggcacac ctgacggtgt caaaaccatt gtgaaatgat ctgacggggg aacctccccc
    29101 gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc acgtttttac ctggctattg
    29161 tcgattgctc taaataaatc ttctaaacca gcatattcat ccaagaatta cctttgcgtg
    29221 atatttcctc aacatcgcga cgcaaacgtt catattgccg caatattatt ttttgatatg
    29281 ttgaaaggcg gacgcaaatc cgcacacaac atttcaaaag acaggattgg gtaaatggca
    29341 aaggtatcgc tggagaaaga caagattaag tttctgctgg tagaaggcgt gcaccaaaag
    29401 gcgctggaaa gccttcgtgc agctggttac accaacatcg aatttcacaa aggcgcgctg
    29461 gatgatgaac aattaaaaga atccatccgc gatgcccact tcatcggcct gcgatcccgt
    29521 acccatctga ctgaagacgt gatcaacgcc gcagaaaaac tggtcgctat tggctgtttc
    29581 tgtatcggaa caaaccaggt tgatctggat gcggcggcaa agcgcgggat cccggtattt
    29641 aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg tgattggcga actgctgctg
    29701 ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc gtggcgtgtg gaacaaactg
    29761 gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta tcatcggcta cggtcatatt
    29821 ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt atgtttactt ttatgatatt
    29881 gaaaataaac tgccgctggg caacgccact caggtacagc atctttctga cctgctgaat
    29941 atgagcgatg tggtgagtct gcatgtacca gagaatccgt ccaccaaaaa tatgatgggc
    30001 gcgaaagaaa tttcactaat gaagcccggc tcgctgctga ttaatgcttc gcgcggtact
    30061 gtggtggata ttccggcgct gtgtgatgcg ctggcgagca aacatctggc gggggcggca
    30121 atcgacgtat tcccgacgga accggcgacc aatagcgatc catttacctc tccgctgtgt
    30181 gaattcgaca acgtccttct gacgccacac attggcggtt cgactcagga agcgcaggag
    30241 aatatcggcc tggaagttgc gggtaaattg atcaagtatt ctgacaatgg ctcaacgctc
    30301 tctgcggtga acttcccgga agtctcgctg ccactgcatg gtggtcgtcg tctgatgcac
    30361 atccacgaaa accgtccggg cgtgctaact gcgctgaaca aaatcttcgc cgagcagggc
    30421 gtcaacatcg ccgcgcaata tttacaaact tccgcccaga tgggttatgt agttattgat
    30481 attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg caatgaaagc tattccgggt
    30541 accattcgcg cccgtctgct gtactaattc cccttctctg aaaattaacg ggcaggtcac
    30601 tgacttgccc gtttttttat cccttctcca caccgttgcc gcgttgcgca ttctactttg
    30661 cgagtctgct ccagaaaata aatcaaagtc agatcaatgc gttgtgttta tttaatgggg
    30721 atttacgatg gcgggacagc atggggctgt aacggtaaag ccctcaccga agcgagggct
    30781 taaaggagaa gggttatgat gcgacttgtc atcatactga ttgtactgtt actgataagt
    30841 ttcggagctt attaacagtc agtctcaggg gaggagtcat cctcccttac ccttactcac
    30901 taaattaggt caaagaatca acgatgtcaa tcagggcgat gcggggtgca tcgccctgaa
    30961 ccgaactttt tgatagccac ttcatcgccc acgtttactg ccgtaataga tcctttttcc
    31021 caaaaattat ctctgttttg catggggttt ttcgagcacg ctcgaagaaa agatttaaag
    31081 gtagatcaat gcgttgtggt tatttaaaga gagcttacga tagctgggca gcatggggct
    31141 gtaacggtaa agccctcacc gaagcgaggg cttgaaggag aagggttatg atgcgacttg
    31201 ccatcatact gattgtactg ttactcataa gtttcccagc ttattaacag tcagtcacag
    31261 gggaggagca atcctccctt acccttactc actaaattag gttaaagaat caacgatgtc
    31321 aatcagggcg atgcaggtgt atcgccctta ccactcccag actttcgacg gtgtaaccac
    31381 cgcaggaaga gggatatccc actcttcaac ggggagtttt tccaccaact gacaatcatg
    31441 cgcataaccc accggttgcg ttttatagtg ctgccagttt tgtaaggtcc gatcataaaa
    31501 accaccgccc attcccaggc gctgaccgta ctcatcaaag gcgaccagcg gtgtgatcag
    31561 cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc agttttggct catggatctt
    31621 caacctgttc atcaccagtt cgctttgcgg atggtaattc aggaacagca aattaccggc
    31681 actaaaggga tgcaaaactg gaagatatac gcgcttaccg gcgcgccaga gttgttctat
    31741 tagcggctgg gtgtcgagtt caccatcaaa agagaggaat acagcgaccg tatgcgccat
    31801 caccaccggg ggataagtca tcatccgggt agcggcttgt tgacccattt cctgctgttg
    31861 ttccggcgtt aacgcacgac gacgttgccg aatcattttg cggatttctt gtcgggataa
    31921 tgtcagtggg agttctggta gttgcgtcat ggctggtgcc agataagaag ggaatctccg
    31981 agatgccgcc gcaggctgta acccttgaac ccttggttca aggtgaatgt gtcgtcgcag
    32041 ttttaaggct tctcggacgg accgagcatg ctcaccaacc gcggagcgcc acattcttgt
    32101 ggtatgaaat atcggctcag gggactggcc cgcttgcgaa catctcagag aaattttgtc
    32161 ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt caaagttttg gttagttttt
    32221 tcggtgatgc gaccttgttc aagtaacgct tgttctatgg tctgctgcag catccgaata
    32281 cgctgttcca tacttgccgc gtagtcacga gtcttcgctt tttcttgcgc taactcatag
    32341 ctgatattca atgcggcaat gaagaccaac tgttcagtat ttgtgactct agtgcgttct
    32401 ttcagatctt gcaaccgttg gttcagatcg tccgctgcct gattcaacgc atccctttgg
    32461 tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt ggatatcgac gggttgtgca
    32521 gacatgccac cttcctgctg attgactgcg ctgccttcgt ctttagaccc atggtctgcg
    32581 aaggggcgac actatagcta ccctgatgag aagagacaag cccttttctg gtccaccagg
    32641 gcccaaagtg gtagcatatc atgaatattc ctccctttga cgacgaatgc ttatgtctat
    32701 acagaacgaa atgcctggtt acaacgaaat gaaccagtat ctgaaccaac aagggacggg
    32761 tctgacccct gctgagatgc atggtttaat cagtgggatg atatgtggcg gtaacgatga
    32821 cagctcatgg ctaccgctac ttcacgacct gacgaacgaa ggcatggctt tcggtcatga
    32881 gctggcacag gcactgcgta aaatgcactc tgccaccagc gatgccctgc aggatgacgg
    32941 cttccttttt cagctttatc tgcctgatgg cgatgatgtc agcgttttcg atcgggctga
    33001 tgcattggca ggttgggtca atcacttcct gcttggtctt ggcgttacgc aaccgaagct
    33061 ggataaagtg accggcgaaa ccggtgaagc tatcgacgat ctgcgtaaca ttgcgcaact
    33121 gggttacgac gaagacgaag atcaggaaga gcttgaaatg tcgcttgaag agatcatcga
    33181 atacgttcgt gttgccgcgc tgttatgcca cgacaccttt actcatccgc aaccgaccgc
    33241 gccagaagta caaaaaccga ctctacacta aaaacaaaaa acgtaaggag agtgttatga
    33301 gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc cctggtggag caaatgcaac
    33361 ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac acgtagcgcc gacagcgaat
    33421 acccctatcg tcagaacagt gacttctggt acttcaccgg ctttaacgaa ccggaagcgg
    33481 tgctggtgct gattaaaagc gatgacactc ataaccacag cgttctgttt aaccgcgttc
    33541 gcgacctgac ggcggagatc tggtttggcc gtcgtttagg tcaggatgcc gcgccagaga
    33601 aactgggcgt tgaccgcgca ctggcattca gcgaaattaa tcagcaactt tatcaattac
    33661 ttaatggtct ggatgtggtt taccacgccc agggcgaata tacctatgct gatgaaatcg
    33721 tgaacagcgc gctggaaaag ctgcgtaaag gttcgcgaca aaatctcacc gcgccggcaa
    33781 cgatgatcga ctggcgtccc attgttcatg aaatgcgcct gttcaaatcg ccagaagaga
    33841 ttgccgtact tcgccgcgcg ggtgaaatca ccgccctggc gcatacccgg gcgatggaaa
    33901 aatgccgtcc gggaatgttc gagtaccacc tggaaggcga aattcaccac gaatttaacc
    33961 gccacggcgc gcgctatcct tcctataaca ccattgtcgg cagcggtgaa aacggctgca
    34021 ttttgcacta caccgaaaac gagtgtgaac tgcgtgacgg tgacctggtg ttgattgacg
    34081 cgggttgtga atacaaaggt tacgctggcg atattacccg caccttcccg gtcaacggca
    34141 aattcactca ggcccagcgt gaaatctacg acattgtgct ggagtctctc gaaaccagct
    34201 tgcgcctgta tcgtccggga acttccatgc aggaagtcac cggtgaagtg gtgcgcatca
    34261 tggttagcgg cctggtaaaa ctcggcattc tgaaaggtga tgttgatgaa ctgatcgctc
    34321 agaacgccca tcgtcctttc tttatgcatg gccttagcca ctggttaggg ctagatgtcc
    34381 atgacgttgg cgtttatggt caggatcgct cgcgcattct ggaaccgggc atggtactga
    34441 ccgtagagcc agggctgtat attgcgccgg atgcagaagt gccagaacaa tatcgcggta
    34501 tcggcattcg tattgaagac gacattgtga ttaccgaaac cggtaacgaa aacctcaccg
    34561 ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat ggcagctgcg agaaagcaat
    34621 gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg ctggcgctgg ctatttcccg
    34681 gttaagtcac ggggcgctgc cggtacattt gattgaagcg accgcgccag agtcacatgc
    34741 tcatccgggc tttgatggac gagctattgc gctagcggcg gggacctgtc agcaactggc
    34801 gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc gccatcacta ccgtgcatgt
    34861 cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca gaagattacc aactggcggc
    34921 gctggggcag gttgtcgaat tgcataatgt cgggcaacgg ctgtttgcat tgctgcgaaa
    34981 agcacctggc gtaatgctgc attgccccga tcgcgtagct aacgttgccc gtactcagag
    35041 tcacgttgaa gtgacgctgg agagcggcga gatgctgacg ggccgcgtgc tgatagccgc
    35101 tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt gattggcagc aggagcctta
    35161 cgaacaactg gccgtgattg ccaacgttgc gacttccgtt gcgcacgaag gccgcgcttt
    35221 tgaacgcttc acgcaacatg gcccgctggc gatgttgccg atgtctgacg gacgctgttc
    35281 gctggtctgg tgtcatccac tggaacggcg cgaagaggtg ctgtcgtgga gcgacgagaa
    35341 gttttgccgt gaactccagt cggcctttgg ctggcgactt ggaaaaatta cccacgccgg
    35401 taaacgcagt gcttatccgc tggcgttaac ccgcgccgcc agagctatta cccatcgtac
    35461 cgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt gccgggcaag ggtttaacct
    35521 cggtatgcga gatgtcatga gccttgcgga aaccctaact caggcgcatg agcgcggaga
    35581 agacatcggt gattacggca tattgtgccg ctatcaacag cgtcgacaga gcgatcgcga
    35641 agcaaccatc ggcgtcacgg acagcctggt acatcttttt gccaaccgtt gggcaccgct
    35701 ggttgtcggg cgcaacatcg gactgatgac gatggaatta ttcaccccgg cacgcgatgt
    35761 gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg cggttttaag gaataaacaa
    35821 tgcaaagtgt tgatgtagcc attgttggtg gcggcatggt ggggctggcg gttgcctgcg
    35881 gcttacaggg gagcggctta cgcgttgccg tactggagca gcgcgtaccg gaacctctgg
    35941 cggcggatgc accaccacaa ctgcgcgttt cggctatcaa tgccgccagc gaaaaattac
    36001 tcacccgtct tggcgtctgg caggacattc tctctcgtag ggccagttgt tatcacggta
    36061 tggaagtgtg ggacaaagac agttttggtc acatttcgtt tgacgatcaa agcatgggct
    36121 atagccatct tgggcatatc gttgaaaact cagtgattca ctacgcgttg tggaacaaag
    36181 cgcagcagtc gtcagatatc actctgttgg cccccgcaga attacagcag gtcgcctggg
    36241 gagaaaatga aactttcctg acgctgaaag atggcagcat gttaacggcg cgtctggtga
    36301 ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc agatattccg ctgactttct
    36361 gggattatca gcatcacgcg ctggtagcga ccattcgcac ggaagaaccg catgatgcgg
    36421 tggcgcggca ggttttccat ggcgaaggca ttctggcctt tttaccgctt agcgatccgc
    36481 atctttgctc aattgtctgg tcactgtcgc cagaggaagc gcagcggatg cagcaggcaa
    36541 gtgaagatga atttaatcgc gcgttaaata tcgcttttga taatcgcctg ggcttatgca
    36601 aggttgagag cgcgcgtcag gtgttcccac tgacggggcg ttatgcgcgc cagtttgccg
    36661 cgcaccgtct ggtgctggtg ggcgacgccg cgcataccat tcacccgctg gcggggcagg
    36721 gagtaaatct cggctttatg gatgctgcag agctgattgc tgaactgaaa cggttgcatc
    36781 gtcaggggaa agacatcggg cagtacattt acctgcgccg gtatgagcgt agccgcaagc
    36841 acagtgcggc gctgatgctg gctggtatgc agggattccg cgatctgttt tccggtacca
    36901 atccggcgaa aaaactgctg cgtgatattg gtttgaaact ggccgacacg cttcctggcg
    36961 ttaaaccaca acttatccgc caggcaatgg gattaaacga tttgcctgaa tggctgcgtt
    37021 aaaaatttct cttctgctgt ttatttgata cccatcacac tttcatctcc cggttttttc
    37081 gccgggagat tttcctcatt tgaaataaac t
//
LOCUS       DAZGXZ010000036        36863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000036 DAZGXZ010000000
VERSION     DAZGXZ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.036"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..478)
                     /locus_tag="ACHKJF_001914"
     CDS             complement(<1..478)
                     /locus_tag="ACHKJF_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HFP0949280.1"
                     /translation="MAKMIPAFGPIDNNSYGEKTVYTLLKEGLSDDFTVIHSLPWLSA
                     ATRNLGMKLPPSGEIDFLVLHPVYGVLALEVKSGIYRVEGTVFVHIKTKKHVDIVKQT
                     RNNVHGLARWLGGATTLRLRIGYGFIFPDSYFDDKIINAVMVDASVKPFRGIFVDKS"
     gene            complement(883..972)
                     /locus_tag="ACHKJF_001915"
                     /pseudo
     CDS             complement(883..972)
                     /locus_tag="ACHKJF_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1115..1690)
                     /locus_tag="ACHKJF_001916"
     CDS             complement(1115..1690)
                     /locus_tag="ACHKJF_001916"
                     /inference="COORDINATES: protein motif:HMM:NF023902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFP0949281.1"
                     /translation="MLSADQIAQLSRTRSLLNHTSEWIPLSGQQITRLTELPLAYNLQ
                     RSAQLLQQLRVLFPDNPRVQEMVDNWQKSVRSRALPEEAMTGWNEGMTRLQQLAERLN
                     RLDEQRGKYMTVSELKTEVFGIMQAFNRHIPAEEQLRRYNEVRNQNGSEQQQKLTQDA
                     LTELLNRYWFLRHGDAGKKTINNKQDNQINI"
     gene            complement(1724..1873)
                     /locus_tag="ACHKJF_001917"
                     /pseudo
     CDS             complement(1724..1873)
                     /locus_tag="ACHKJF_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2198..2512)
                     /locus_tag="ACHKJF_001918"
                     /pseudo
     CDS             complement(2198..2512)
                     /locus_tag="ACHKJF_001918"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
     gene            complement(2567..2872)
                     /locus_tag="ACHKJF_001919"
     CDS             complement(2567..2872)
                     /locus_tag="ACHKJF_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949282.1"
                     /translation="MNPLFNDIQMRLFYLNHSPYSWHWNVRFRPQEAVYIGSDTCHIT
                     ITCNQSGFHLTRDGQRLFTERYIRNLNELLPVLKRRWDVTPAIIRAVEYLSRVPVLH"
     gene            complement(2938..4683)
                     /locus_tag="ACHKJF_001920"
     CDS             complement(2938..4683)
                     /locus_tag="ACHKJF_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0949283.1"
                     /translation="MTPGERRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVSTLRQVNQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPANPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEVFQQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEARGL
                     ASRVTVSHFHSWCASMVKRHGIKVTADRKEYPDKCFSALEEAVNSGKITRTGYDAVLV
                     DEGHDFDSRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASERDE
                     ARQVADWLEKRHALCGHWSDMAVLCPTERSAQQLQDVMTLRGVPFATCFDREGKKTYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            complement(4918..6095)
                     /locus_tag="ACHKJF_001921"
                     /pseudo
     CDS             complement(4918..6095)
                     /locus_tag="ACHKJF_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242985.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(6292..6594)
                     /locus_tag="ACHKJF_001922"
     CDS             complement(6292..6594)
                     /locus_tag="ACHKJF_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949284.1"
                     /translation="MSHCTSDGRFVCNDGSLSQSKRFCSGYGVAPAKRQVKSPVTTKL
                     VQKKKSAAVRKVSQPSVAGKNEVVPVTEPRKPTCAPLYMASKPGFTHLPICSGNQY"
     gene            complement(6941..7945)
                     /locus_tag="ACHKJF_001923"
     CDS             complement(6941..7945)
                     /locus_tag="ACHKJF_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0949285.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARRISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTEPLQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPTGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPVTASLLSSQPGDGKQFSCSRDFAASTGLVPRQYSTGGKLTLLGISKRGDKNLRRLL
                     VQCARSFMMRLERQHGKLAEWVREQLNKKHSNVVACALANKLARIAWVITTQQKEYQA
                     "
     gene            complement(8203..8634)
                     /gene="tssE"
                     /locus_tag="ACHKJF_001924"
     CDS             complement(8203..8634)
                     /gene="tssE"
                     /locus_tag="ACHKJF_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0949286.1"
                     /translation="MPRPSLYEILYGNFAGGLELNQVSAEEQVILSVLDNMQRILNTR
                     AGSLKHLPDYGLPDMTTVLQGMPGTAHQLMRVLSDVLLKYEPRIKRVNVTMQEQTQPG
                     ELHYVIDAELKDAGLVRYGTTFIPEGRVLLRHLKQQRYVQT"
     gene            complement(8637..9173)
                     /gene="tssJ"
                     /locus_tag="ACHKJF_001925"
     CDS             complement(8637..9173)
                     /gene="tssJ"
                     /locus_tag="ACHKJF_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0949287.1"
                     /translation="MAIIAGKAGYGLIIALFSLSLSGCGLTQRVADGTVSATKSLFYR
                     QIKTLHLDIRAREAINTSAAGIPLSVVVRIYQLKDNRNFDSADYQALFTGDSEILAGD
                     IIAQKDVWMQPGGSVTVDMPLDDAAKFTGVAAMFLEPDRKKNTWRVVLSRDELEPDTP
                     RLIEVSGNTLTLLPVKDK"
     gene            complement(9154..10254)
                     /gene="tssG"
                     /locus_tag="ACHKJF_001926"
     CDS             complement(9154..10254)
                     /gene="tssG"
                     /locus_tag="ACHKJF_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0949288.1"
                     /translation="MHPVERKSQSAPARLITRYRKQLPYINFYRFCQLLEQSQPDQPP
                     IGSGWQARQEAVRFCPYPGMGFPASEIKDAVIPEESHLPPTVHVTFMGLYGVTSPLPA
                     HYISDIAQQREGHEAAADFLDIFSHRLITQYYRIWRKYSYPATFEAGGQDKTSQYLLG
                     LARLGIPGCAQNIATPVSRFLALLPLMLLPGRTAEGLTSLVTLLAPGTQARVWHHDRR
                     RIPLKTPLAMRVHHPVSLKSRPVMGDHATDVNGQVLLQLSTQTGSEVQGWLPGGQLYS
                     DLLALLHVYLGSRLDVRLQLCVERSLLPDVRLSCRPAAGSPQLGRTAVMRTQAKITTS
                     AARVMTIRLGRYQRVQEHYQRKEAQENGDYRW"
     gene            complement(10209..11972)
                     /gene="tssF"
                     /locus_tag="ACHKJF_001927"
     CDS             complement(10209..11972)
                     /gene="tssF"
                     /locus_tag="ACHKJF_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0949289.1"
                     /translation="MDDLTLRYFDAEMRYLREAGKAFAQAHPDRAAMLDLDKAGTPDP
                     CVERLFEGFAFSMGRLRQKIDDDLPELTESLVSMLWPHYLRTIPSLSVVALTPRLSVM
                     KMAETVPAGLEVTSRPVGPGNTVCRYRTTRAIPLNPLAVEKVVMTTEPDGRSVLKIGF
                     ACSELADWSQVDLHRLSLYLAAEAPVSSTLHLMMTKRLAALYLRLPGNDERIRIDGWF
                     SPGGFAEEDRLWPKGDSAFSGYQLLLEYFTFREKFMFVHLNGLENISLPAGISGFDIE
                     VVLSQSWPADLPVTDDALCLHCVPVINLFTLEADPLIINGLESEYLLRPKRLQDGYTE
                     IYSVDAVAGSGRTGSAEYVPFTSFRHRGGMLRHDAPERYYHTRVKRGVTGMYDTWLIL
                     GGQRWEVDRMPERETLSLRITGTNGQLPRRALQSTLLDRCEQVLQTPVSVHNLCKPTL
                     PVYPPTEDRFHWRVMSHLGTGFLNMLSSAEVLRGTLALYNWRDDELNHRRLDAILAVQ
                     HHRIQRFEKGFLLRGLDVEVTLDGNGFAGEGDIHLFGEMLNRFLALYADMNQFNQLTL
                     IVQPEGKCIRWKENHNPRLPG"
     gene            complement(12106..13704)
                     /gene="tssA"
                     /locus_tag="ACHKJF_001928"
     CDS             complement(12106..13704)
                     /gene="tssA"
                     /locus_tag="ACHKJF_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0949290.1"
                     /translation="MASIHSLLSACQTTPRDVAEPAQVRIALWDKWLAPVTPDNPAGD
                     DAGYDDDFQQMREEVNKLSGADAGIVSQLAEKLLTTRTKDIRVATWYIWARLRQDGEK
                     GLADGLELLAGLLQRFGEHLHPQRARARKAALEWLCSARILDSLSLYPEVVKADTLRI
                     AGVLWLAEQTFTDEASAPVLNGLYQALENRLMKAGGVDAVVPQEAAAPAPTVTSGSVM
                     ALSAITSGQELLSQARVLAKYLRDQPEGWLAAHRLMKSVRHDTLHQLPPLSADGRTRI
                     APPGPDRRASLKRLYLQQNWLSLLEQCDDMFARGASHLWLDLQWYIHQALLQTGKENY
                     AAIIQYDLKGLLLRLPGLETLAFNDGMPFADDVTLSWIQQQVMECGERWAEEPSVTIT
                     AAPGDNDILSLEPEALQIADNEGTEAALSWLQARPGIQSDRSNWLLRLLMARVAEQTG
                     KNDLALHLLVELDERATRLTLSQWEPELVFEVKARRLKLLRMKSAKTESDRVRLQPDM
                     EHLLAGLIAIDAARAAVLCNSGSS"
     gene            complement(13704..17093)
                     /locus_tag="ACHKJF_001929"
     CDS             complement(13704..17093)
                     /locus_tag="ACHKJF_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFP0949291.1"
                     /translation="MNKLACLSGRFGRPGIVFIGVAALWWLITRYGAFLGAETRRDQI
                     LLLILLSLGLLFVCYLPVMKKYVQELTYRRRARKEQRLPDDEERLAQTPPRYVTVQDI
                     RHTLRRQYGRFWGRKIRILLITGTASEVELLTPGLTEQFWQEEQGTLLLWGGEPSQPE
                     NADWLAALRRLRYRPADGIVWVTSGLSETLSAPLTEDALDRVSRAVSSCCERLGWRLP
                     LYVWSLQESPDERGRITQPVGCLLPAECSSDKLKAQLQAMLPGLVAQGIQQICCAPRY
                     YFLLSLAERFRRNIDAVVEPLSVLLRPYRQLLLAGIVFSPATVGGERSVRHRWRMDNR
                     WEALPETVQQLPVRLQPSRTGHNWRRSLAVMAAILMMAQGTGMVVSFLANRSLVAEVQ
                     EQIRPAQNQQLSPAERLQALLNLQKSLARLQYREEHGAPWYLRAGMNQNADLLAVVMP
                     LYAQNAHLLLRDAAAAHLEQQLRTFIRLPPDSPQRGKMAKAAYDQLRLYLMLAQPQHM
                     EPAWFSRTLMREWPQRDGVSAVFWQANGPTLLAYYASGIITHPQWKLTADEELVSQSR
                     TLLLRHLGTQNSDAMLYQKMLARVAHQFADMRLTDMTGDTDVSRLFFTDEVVPGMFTR
                     QAWEEAVLPSIDTVINERREEMDWVLTDGRQKAPSPVSPEALRQRLTTRYFADFGNAW
                     LNFLNSLHLRKAQMLSDVTEQLTLMADVRQSPLVALMNTLAVQGRTGQPREAVTDSLV
                     KSARNLLSQEKQPVAVPESRLHGPLATTFGPVLALMDNQNNSADMLNLQTYLTRVTQV
                     RLRLQQIAGSSDPQAMMQMLAQTVLQGKSVDLTDTRDYGSLTAAGLGQEWYGFGQTVF
                     VRPMEQAWQQVLTPAAESLNARWRTAVVDGWNNAFSGRYPFKNVSSDASLPLLAKYLN
                     TDTGRIARFLQNNLSGVLHREGSRWVPDTINTRGLTFNPAFLKAINTLSEIADVAFTT
                     GNAGLHFELRPGTAAGVMQTTLITDNQKLIYVNQMPVWKRFTWPADTEAPGASLSWVS
                     TQAGTRQYADLPGSWGLIRLLEMARRKAAPGVASGWSLSWQAQDGRMLNYTLRTEAGE
                     GPLVLLKLRNFVLPETVFELSGTSAFTGNDEDAGDTVEETD"
     gene            complement(17086..18234)
                     /locus_tag="ACHKJF_001930"
     CDS             complement(17086..18234)
                     /locus_tag="ACHKJF_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP0949292.1"
                     /translation="MGWSRTKSLSTELPHKPSMMLWFMTGAFMAVVGALLFIIRASEY
                     VKALNDFSIWWLALTPPGGWFFLFCLRHWQWSNQMDEHLFLKKEGEYAQKQWESWAER
                     YLVITASCVYLPDKITVATLCDELPLQYGLVKKIDYLSDSGHKVEASLRVLLREITDK
                     FCQLPAVLPVNVTLITDQPDSEIRSAFVSAWEALFPQRVVPDNIEVTPDFSMGWVDER
                     LKQPVLTVDLILVIQLNGGNAYSDGLAALLLTSDDVAQKYNLPHPARLLRPMSLDINK
                     FNDEFTLFLETQTAACRTARVLGDCYHWEKIAAPLMTIGNQYGAGWEPSGRMLLEKWC
                     GIPGPAAPWLLTALAADLVCLGNDSLLTLFSSGEEHFISTVTSGNQNE"
     gene            complement(18238..18504)
                     /locus_tag="ACHKJF_001931"
     CDS             complement(18238..18504)
                     /locus_tag="ACHKJF_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFP0949293.1"
                     /translation="MSKGFVLLGDNTTHGGKVISASSTMIVDGKKVALVGDMVSCPVS
                     GHGTNPIVEGSPEWISDGRAVVIDGCKCLCGCKVISSAPECAIG"
     gene            complement(18535..19212)
                     /gene="tli1"
                     /locus_tag="ACHKJF_001932"
     CDS             complement(18535..19212)
                     /gene="tli1"
                     /locus_tag="ACHKJF_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS immunity phospholipase A1-binding
                     lipoprotein Tli1-EAEC"
                     /protein_id="HFP0949294.1"
                     /translation="MTRDKIIFIAPLLALLTGCNSVSSQYADSDKTEWGYKKLPYDEW
                     KFLFIYPKALPALATQALMVDGEGYKTTYQYLDNTEPSQVSVGRWSDHLGATESYYNK
                     GKALPLMLRFCWDSIIDKKSYETLIFFKKGTWEQMVTPYTDNRWNETYYRSTMIIGLA
                     PEGKVRVWLGDRGNPVVPQLDAKITTVSGDKMNMCKGVTKHPDGYVYYGDTPDFIKGK
                     IYPYGNW"
     gene            complement(19232..20914)
                     /gene="tle1"
                     /locus_tag="ACHKJF_001933"
     CDS             complement(19232..20914)
                     /gene="tle1"
                     /locus_tag="ACHKJF_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T6SS effector phospholipase Tle1-EAEC"
                     /protein_id="HFP0949295.1"
                     /translation="MTKYQGYDVTDATHKTSIHNDWKVVVAKKKPARGVTLTIGIFFD
                     GTGNNRENTASRLMKFNECSAARQGVNQKDAQSCEDFLEEINKNNISNGSYRGYYSNI
                     HWLNILYHPDQVLKKDQTSAQIKTYISGIGTAAGEADSVIGMGLGTSILDIFEGVVTK
                     TDEAMERITQALSEFMGFNLNPDFCIAKIQFDVFGFSRGAAAARHFANRVMEQDPAIA
                     RAIAKGLRGDFYDGKPSGEVRFLGLFDTVAAIGGISNFFDINGRSNPGVKLELRPSIA
                     KKVFQITAMNEYRYNFSLNSIKGMWPELALPGAHSDIGGGYNPVGSPLQENESLFLSC
                     PAFEIVSDDTRETDTRVYRNAEQARKMLMTLPALKHILPHGKLTTKIRSVGVNNSNQR
                     RAGVIQKQVGAAVFFERMAVPNDWANVCLRVMLDAAQEAGVLFAPIDPKNPDMNLSPE
                     LIPFVDKAIAQGKAVRLGKEPQAFTEEELYIIGKYTHCSANWNIESDGNLWVDPTTGE
                     IFIHRFGPKGNKAFVFPNKPNDRWIRSVWYMDDQQRLNDNAVKNTKVMMSGV"
     gene            complement(20911..23430)
                     /locus_tag="ACHKJF_001934"
     CDS             complement(20911..23430)
                     /locus_tag="ACHKJF_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0949296.1"
                     /translation="MSLTDTLSKTISASLNRYHLIIPSCPSPLDVEHFSGREEMSQTY
                     YYIINFTSTDNDLDAGLLLRKSATFTMGTGGLKEQVSQKVVHGVITDFRRISGSADQA
                     KYQVILEPFIKLLDRQHRSHRFFVNKSVPEVVTEVLQEHGLKGWEFEFRLKKTYPKRE
                     QINQYQESDLQFIQRLLAEVGIFYFFTLQPDTQTEIVHFGDSQAALTFDKTLAINSPS
                     GMNDNRADSVWGLNVTHNVAEASVTTKAYNHREAQYLLQSAPADMTHGDGDGINYGEV
                     YHYHFRHLERGDKIDPVPETANFYARLGHERYLAEQVRITGNSTDATLAPAQVLTITD
                     SLPPTLPALLRNPVLLTCVGFSASRKDALQVVLKGVPYSEVICWRPPLLPRPKITGTM
                     TARVTSAKEGDIYAWQDASGMYRVKFDADRDDKNPGQESMPVRLAKPYSGDAYGFHFP
                     LIQGTEVAIAFEEGDPDRPYIAHALHDSRHVDHVTDKNGTRNVIRTPANNKLRMEDKR
                     GEEHIKISTEFGGKTQLNLGHNVNASRKLRGEGAELRTDDWISIRGGKGIFISADMQP
                     QAQGKMLDMNEAIRQLEQALSLARSMAKAATAANATQGDISCQQRLNSSLTDLTAPGM
                     LLHAPDGIGMVSARALRIASGSESVGIMSGDNTDISAGQSFTVVAEGAVSLLSRNQGM
                     QLLAAKGRVNIQAQSDDLSMSSQQNLDIQSSEGKVTVSANQELILACGGAYIKLSGGN
                     IELGCPGQILLKSTNMQKMGPTSLDIASVEMPRGFGGGFILTDEAGVPQPSTPYRLTT
                     AEGDILQGITDENGKTAPVNTSIPSVVKVEFGKVKIHGETE"
     gene            complement(23509..23736)
                     /locus_tag="ACHKJF_001935"
     CDS             complement(23509..23736)
                     /locus_tag="ACHKJF_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949297.1"
                     /translation="MKIKRTLLIMLSRFIRGAGMGLGASGIVFTVWFFFLSSAEFKYL
                     WGGLSVALFFVGYLMYRFAYAYIYDEWDDYQ"
     gene            complement(23733..24374)
                     /locus_tag="ACHKJF_001936"
     CDS             complement(23733..24374)
                     /locus_tag="ACHKJF_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949298.1"
                     /translation="MDSAKEIARDMLEAVNINGDGFLGAVGKGLISLPVSLGYMGYDF
                     IDTGHRRENQNDKFRMARLVEKGIFSRDIIGKIISICLEDFVSRINMEKTGSIVKNVS
                     GDLAGRMAFAELTGVKLGEVLVSRGLAAFFAGSFAGLLLSVGAETSCAIYTSRFLKDR
                     NPVLHDKLKAQGDLDLLYFLVEDIVRPFERACIVADRNSAEFDRICEYFFGGL"
     gene            complement(24361..27048)
                     /gene="tssH"
                     /locus_tag="ACHKJF_001937"
                     /gene_synonym="clpV"
     CDS             complement(24361..27048)
                     /gene="tssH"
                     /locus_tag="ACHKJF_001937"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0949299.1"
                     /translation="MENPAALLRRLNHYCARALEGAASLCQTRAHAEITPEHWLLKLL
                     EQGEGDLTVLGRRYDWDMDAIWQSLLGWLDNQPRSVRSRPQLAQSLNALLKQAWMVAS
                     LQGEEHIRSVHLLGALTENPHLVRCDGVWPLLTLSQSQLQRLSPLLDAQSDECPERLQ
                     DAEPVLPQGENVTFIGRPVGADTAGIPSGDLPPVLQSALDKFTRDITASAREGKIDPV
                     SGRDTEIRQMVDILSRRRKNNPILVGDPGVGKTALVEGLALRIAEGNVPESLRPVTLR
                     TLDLGLLQAGAGVKGEFEQRLKNVIDAVQQSPAPVLLFIDEAHTLIGAGNQAGGADAA
                     NLLKPALARGELRTIAATTWSEYKQYLERDAALERRFQMVRVDEPDDETACLMLRSLK
                     SRYAEHHNVHITDEAVRAAVTLSRRYLTERQLPDKAVDLLDTAAARVRMSLDTVPEQL
                     TRIRSQLASLGMEKQALLEDIAVGHQNHGERLSAIEQEENVLMTARDDLEQQYARECE
                     LTGELLESRSDISRQSETLHLQQALHDIQQNQPLLSVDVDVRTVAGVVADWTGVPLSS
                     LMKDEQTELLHLEKDIGRRVVGQDVALGSIAQRLRAAKTGLTSGNGPQGVFLLVGPSG
                     VGKTETALALADVMYGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGILTEAV
                     RKRPYSVVLLDEVEKAHRDVLNLFYQVFDRGFMRDGEGREIDFRNTVILMTSNLGSDL
                     LMQQLSEKPETTESELHELIRPLLRDHFQPALLARFRTVIYRPLTQSAMRIIVEMKLA
                     QVCERLHCHYGLSTSVDERVYDALTSACLLPDTGARNVESLLNQQLLPVLSRQLLSYL
                     AARQHPTYLSLTWHDEDGIVIAFDGINSDYPQESIYGQC"
     gene            complement(27216..27707)
                     /gene="hcp"
                     /locus_tag="ACHKJF_001938"
     CDS             complement(27216..27707)
                     /gene="hcp"
                     /locus_tag="ACHKJF_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system effector Hcp"
                     /protein_id="HFP0949300.1"
                     /translation="MAIPVYLWLQDDGGADIKGSVDVKDREGSIEVVAQEHCLYIPTD
                     NNTGKLTGTRIHTPFLFTKEIDSSSPYLYKAVTTGQTLKSAEFKWYRINDAGQEVEYF
                     NTKLENVKVVKVNPVMHDIKNPAYEKHNHLEQVELRYEKITWTYKDGNIIHSDAWNER
                     TTA"
     gene            complement(27742..29442)
                     /locus_tag="ACHKJF_001939"
     CDS             complement(27742..29442)
                     /locus_tag="ACHKJF_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0949301.1"
                     /translation="MHVRSRLWLTILSLFLTLWLISGFLPLSAVNQIILTLVSVVAAG
                     LVLWYQWRCLSSRRDIPENAGDDGLPPEYFQGHVLLVAGNSDAWFEPGQAYRETASGW
                     YLRVDTPEQMRILAERLSYSRPALCGQVVVMLGLLPEQHTSDEAFLSWLRNWQRAVMQ
                     CRQWLNTMPPVLVTLMVSGPSPQNTSLNKHSAHWFTLTPDLSEMHIRLTEAGAIPAEE
                     WLRECDAASRLRCVSELLWLNAMLSWYNRVTDALTDICRQEHFYFRPVAVGFCLTSVA
                     TRPGNLWQQQLTSLTVLPPPAGVSSQALPLPDILLSGIPRHRGLSHWHRLCLQTGAIV
                     GVFLLLAMLASFMNNQRLVRSVGEHLTAWHRMRGYSSESKKTALQRLQADSQLLNDWQ
                     QRGVPQRYGLGLYQGMWLIPPVERATGEWIAPLPPRPVIQKVTQTPKMVRLNSLALFD
                     AGKWTLKPGATKWLVNALVDIKAKAGWLIVVSGHTDNTGDPQRNQALSLKRAEAVRDW
                     MRDTGDIPQSCFAVQGYGESRPVAPNETAEGRARNRRVEISLVPQADACRLPDITRAP
                     "
     gene            complement(29445..30098)
                     /gene="tssL"
                     /locus_tag="ACHKJF_001940"
     CDS             complement(29445..30098)
                     /gene="tssL"
                     /locus_tag="ACHKJF_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFP0949302.1"
                     /translation="MNKPVISRAEQIFYPGWLMVSQLRCGQPVEDGKALYRRACQLVT
                     QAREELAEAEFSQESSEIMLYAFCALLDESVLNREKTDDGWRTWQQDPLQAHFFGTLN
                     AGEELWERIREQLKLPAPDVAVLTCLCRTLQLGFTGQYRSQDDERREDVIRALTARVP
                     AFTFAQDAPVVVRAPGYRVGRTMYWLSWVAGIVTLAGLWCVLSSVLADQVARLTGQG"
     gene            complement(30095..31432)
                     /gene="tssK"
                     /locus_tag="ACHKJF_001941"
     CDS             complement(30095..31432)
                     /gene="tssK"
                     /locus_tag="ACHKJF_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0949303.1"
                     /translation="MKIYRPLWEDGAFLMPQQFQQQAAWDVHLADSVARMGLAHPWGV
                     VAAEFDDSLLPLSRLNATRLIVRFPDGTLIDTERADNLPPVCDLSTVSERSLVDIVLA
                     LPLLNANGGNLDDGSESERPRRWKSERVNVQELAGHEQSEVAVLRHNLTLRMAHQENA
                     AWLTCPVTRLVRDAQGQWCRDPRFIPPLLTLSASPSLMTELAELLHHLQARRQRLMSM
                     RRENNARLADFAVADVSLFWLLNALNSAEPVLKELLDMPYRHPELLYRELARLAGSLL
                     TFSLEHNVDAVPAYRHETPENVFPPLLSLLNQLLEASLPSRVVFIELKQNGVMWEGAL
                     HDARLREGADFWLSVRSSIPGHELQTKFPQLCKAGSPDDVSEVVNVALSGVIIRPVTH
                     VPAAIPLRLENQYFALDLSTDAARAMLDAGRCTFYTPASLGDVKLELFAVLRT"
     gene            complement(31448..32992)
                     /gene="tssC"
                     /locus_tag="ACHKJF_001942"
     CDS             complement(31448..32992)
                     /gene="tssC"
                     /locus_tag="ACHKJF_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017892320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0949304.1"
                     /translation="MLMSVQKENNVAGNAVSETHSGDSVYASLFEKINLNPVSALSAL
                     DIWQDPQAMSEASADERLTAGMQVFMECLAKAGAPVEKLDKALIDHHIAELDYQISRQ
                     LDAVLHHPEFQKVESLWRGVKSLVDKTDFRRNVKIELLDLSKDDLRQDFEDAPEIIQS
                     GLYLQTYVAEYDTPGGEPIAALISAWEFDASAQDVALLKNISRVAASAHMPFIGSVGP
                     AFFRKDTMEEVAAIKDIGNHFERAEYIKWNAFRETDDARYIGLVMPRVLGRLPYGPDT
                     VPVRSFNYVEEVKGPDHHKYLWTNASFAFAANMVRSFVTNGWCVQIRGPQAGGAVQDL
                     PIHLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSTQKPA
                     LYDTPDATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRLLELELNNWIRGL
                     VTEMTDPGDELQASHPLRDGKVVVEDIEDNPGFFRVKLYAVPHFQVEGMDVSLSLVSQ
                     MPKAKA"
     gene            complement(33013..33510)
                     /gene="tssB"
                     /locus_tag="ACHKJF_001943"
     CDS             complement(33013..33510)
                     /gene="tssB"
                     /locus_tag="ACHKJF_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0949305.1"
                     /translation="MSSSFQNEIPKARVNLKLDLHTGGGQKKVELPLKLLVAGDFSNG
                     QEERPLSERIKMDVNKNNFNAVLADISPSVRLNVENTLAGDGTEESISLTFRNMNDFT
                     PEQVARQIPRLKAMLAMRSLLRDLKANLLDNATFRKELEKILRDPELSQALRDELSAL
                     VPEQV"
     gene            34009..34217
                     /locus_tag="ACHKJF_001944"
                     /pseudo
     CDS             34009..34217
                     /locus_tag="ACHKJF_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
     gene            complement(34237..34470)
                     /locus_tag="ACHKJF_001945"
     CDS             complement(34237..34470)
                     /locus_tag="ACHKJF_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949306.1"
                     /translation="MARSDYDIINLSPEHELNEWLAERGYAGQADNRNRVAEIVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(34559..35182)
                     /locus_tag="ACHKJF_001946"
     CDS             complement(34559..35182)
                     /locus_tag="ACHKJF_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0949307.1"
                     /translation="MRTRIDYLADKYSFTELNELPRLHQQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWDISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQIAREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKSGMPVVTTTAEVHDNLTGTTRKVPAYYL"
     gene            complement(35271..35780)
                     /locus_tag="ACHKJF_001947"
     CDS             complement(35271..35780)
                     /locus_tag="ACHKJF_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949308.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSLM
                     PRGIATIIR"
     gene            36237..36695
                     /locus_tag="ACHKJF_001948"
     CDS             36237..36695
                     /locus_tag="ACHKJF_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949309.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIIFLIILLVCIHSLITTIK"
     gene            36795..>36863
                     /locus_tag="ACHKJF_001949"
     CDS             36795..>36863
                     /locus_tag="ACHKJF_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HFP0949310.1"
                     /translation="MEITKNKNATIKKQSSVGCFSMF"
BASE COUNT         9327 a        10254 c         8321 g         8961 t
ORIGIN      
        1 ggcttttatc aacaaatatt cccctgaatg gtttaacaga cgcatctacc attactgcat
       61 ttataatttt atcatcgaaa taactatcag ggaaaataaa accataacca atacgcaacc
      121 gtaacgttgt tgctccccct aaccaacggg ctaaaccatg gacattgtta cgtgtttgct
      181 tcactatatc tacatgtttt tttgttttta tatgtacaaa aacagtacct tctacgcgat
      241 atattccact ctttacctct aacgccaaaa caccatatac aggatggaga acaagaaaat
      301 caatttctcc actcggaggt aatttcatcc ctaaatttcg tgtcgccgca cttaaccacg
      361 ggagactatg aatcactgtg aaatcatcgg acagaccttc ttttaacagt gtatagactg
      421 ttttttcccc ataactatta ttatcaatgg gtccaaatgc cggaatcatt ttggccatat
      481 tactatttcc ttataacatg tttctgacag gaatctgcat ttgttacata gcccagtaat
      541 taaaaataac ttccgcccat tttaaaaatt actcatactt attccgaagc agactctgac
      601 taaccaatat atacaatctc cctccacact ctcatgcagc agccagctaa cagtagttga
      661 ctgcctcaac ttcagggtga cttctgccgt tactttttta acccctcccc caacgcctaa
      721 acgacacttt cgttttctgc agtgcctcat aggaagtatg agcttcaaaa ctggtgtaat
      781 caaaacccgc tgacgctacc attctgacaa atatgcctgt gaatgatacc cgacttttcc
      841 atcatgatgc ctgtttagtc tggcgcgcac cgtacggatg acatctttca cccggccagt
      901 ttcatacaac gcatcgtaag ctttgtacgc atcagcctgc aactctcacc catattctgc
      961 cagatgacgt tgtgaatctt cgagggaaag ccgctttatc atctgcgtat tctgttctat
     1021 ttgatcacag attggtccaa tctgtgatca attcagatgt aattttgcat taatggcatc
     1081 atcgcttctg gataaataat ggcaataaaa tctgtcagat attaatctgg ttatcctgtt
     1141 tattatttat cgtcttctta cctgcatctc catgtctcag aaaccagtag cggttcaaca
     1201 gttccgtcag ggcatcctgc gtcagttttt gctgttgttc actgccattc tggttgcgga
     1261 cttcattata gcgacgtaac tgctcttctg ccgggatatg ccggttaaaa gcctgcatga
     1321 tgccaaacac ctctgttttc agttcactga ctgtcatgta ttttccccgc tgttcatcca
     1381 gacggttcag gcgctcagcc agctgctgta ggcgggtcat gccttcgttc cagcccgtca
     1441 tcgcctcttc cgggagcgca cggctcctta cactcttctg ccagttatcc accatttcct
     1501 gtacccgggg attatcgggg aaaagtaccc tcagttgctg cagcagctgc gcactgcgct
     1561 gcaggttgta tgccaggggt aattctgtca gtcgcgttat ctgttgaccg gaaaggggta
     1621 tccactctga cgtatgattt aacagtgagc gtgttctgct gagctgtgct atctggtctg
     1681 ccgaaagcac atccggtggg ggtgtcagca gcgccctgag ttgctgctcc gccggtgatg
     1741 gctgtgtcag aaactgccat cccggaatgg ccacgctgac agccagcaat gccgcacaga
     1801 caccagccaa aaacggcttc agaaacatgg gcggggattt ctgaagggag ggtgtgggct
     1861 ctgcttttgt ttcagaaaca acatacacca gacgggagtg cggcagggtc tgatgttcct
     1921 gcacatccgg agcatcagac cggagtgcct gagccggaag cgttaccggg gacgacgctt
     1981 cgccctgcac aggctgattt tccaggcggg tgatatatcc cttcaccatg gcttccggta
     2041 aagccacttt actgccctgt ttaagttcgt gccgggcaag tgtgtcactc agtgccttca
     2101 ggaatgtttc ggtctgatac agcaggggca ggttgtcacg gtcgtccgga ggcatggccc
     2161 gaagcgtttt ttgcagacgg ttgaacagac cggtaataat ttccagccgg gcgtgggtat
     2221 tcagcggcca catcactgac cagtgatgcc gggtcagcgc cacaatcaga gccagccctt
     2281 cattcagtcc acttaaccct gctgtatgca tccgggcgat ggtgtaccag gatgccgtct
     2341 gtaattccac gccattgtgt tcatacagac gcaggcagag cgtttccacg taacgccagt
     2401 ccacatccgg acgtgccggg tgggtcagtt tgctgatttc atcacgcagg gcagcataat
     2461 ccggtaatga cctggggtca ccaccggttt tcagtttcac ttcagaagtc atcggcagat
     2521 tcggtgttac tgtattaaag catgattaac agtgacgaaa agaatgtcag tgtaataccg
     2581 ggacccggga aaggtattcc acggcacgga tgattgccgg tgtcacatcc caccgccgtt
     2641 tcagaaccgg taacagctca ttaaggtttc tgatataacg ttcagtgaac agccgttgtc
     2701 cgtccctggt taaatgaaaa ccggactgat tacaggtaat ggtgatatga caggtatcgc
     2761 tgccaatata gacagcttcc tggggtctga atctgacatt ccagtgccag gagtacggag
     2821 agtggttcag ataaaaaaga cgcatctgaa tatcattaaa taacgggttc atggcgaatt
     2881 aaataaacct gataataaca gtatgatgaa agtccggaat aaagaaataa tccggtatta
     2941 tgcaaaatcc tcaaggtgac gactgatact gttttcacgg taacaggtca ccagtaattc
     3001 acgggttgcc cgggtgaacg ccacataaag caccggaatc acttcagact catccgctgc
     3061 accagagtga acgaaagacg cattgataac cgccacatac gggaactcca gccccttgct
     3121 gctctggtac gtcagtaaat gcaccacatc cttacgtcgc gagtatgtct tcttgccttc
     3181 cctgtcaaaa caggtggcaa aaggcacccc gcgtagtgtc ataacgtcct gcaattgttg
     3241 cgctgaacgc tctgtcggac acagcacagc catatcactc cagtgaccac acagggcatg
     3301 gcgcttctcc agccagtctg caacttgtct ggcttcatca cgttcagaag cacaacgcag
     3361 gatttccggc tccgtaccct cttcaccgcc cgcctgtggc tgaacgagcg gtatttcacg
     3421 gttctgatgt ttttcaaaat actcgcgcga gaatgcataa gcaaagtgca gaatgcgttt
     3481 cggattacga taattgaccg gcaggacaga agtgcgtccc tgtgcctgaa tccccacact
     3541 ggccagagaa aagttcaggg cccgctcccg ccggtaaatg gactgggcgt catcgtacat
     3601 caggagcagt gaacgggatg cgttatcaaa cagacgggca atcagggcca gccagcgact
     3661 gtcaaaatca tgcccctcat ccaccagtac agcatcatat ccggttcggg tgatttttcc
     3721 gctgttcacg gcctcttcca gtgcactgaa gcatttatca ggatattctt ttctgtcggc
     3781 cgtcacctta ataccgtggc gttttaccat tgaggcacac cagctgtgaa aatgggatac
     3841 cgtaacccgt gacgccaacc ctctggcttc aatgcattcc cgcagatagc tggccagggt
     3901 aatgttgtag cacaacacca gaatctttcc gggtgtggtt tcagccaggt aaaggcaacg
     3961 gaacaaaaga atcatggttt ttccggaacc ggccacacca tgaatgacac gatgcccgtc
     4021 accaatattt ctggccagaa tctcctgctg aatatccatg attttgattt gtgagttctg
     4081 tttcacggtc acttcaggaa acagatgggc gcgcagaata tctctcatcc ggggtgtcac
     4141 ccgggttctg aatcccgtgg taaacatccc ggcaattttt tgctggaata cctcaggtaa
     4201 aacagactgc gtcatttcat cctggcagat tgtctgtgct gaaggaaaaa ttttttccac
     4261 cgcattctca tcattccctg ccagtgtctt cagttgctga cgggtaatgc gggtaaacac
     4321 cacaccatac gcccaggcca ggttcagccg tcccttatac tgaccgtcat tctgccgtaa
     4381 gcacgggttt gcaggtaagg cattaaccgt atcacaggca taacgacgaa cctgtaccag
     4441 cggattgatt tctgatttca gaagaccgtc ggtttccagc gtgacctgct cctgatttac
     4501 ctggcgcagc gtggaaaccg tccagtcctt gacctccaga aataccagtc cgttctccgg
     4561 gtcaataatg acaaaatccg gatgccggtt tttccgtccc accggaatat cgtaccagac
     4621 aaggcagtca tcattcagaa agctctccag tcgtgaagcc accctcctct ccccaggcgt
     4681 cattctctcc tcagtggagg ccgatacatt tatcagtttc gccataaacg caccttaatc
     4741 tgtgaacaaa aaataatcac caccgacatc cacggatgtt atatcgtggg tgtcgcttta
     4801 ttcagaaata aaaaacagaa tcattaacat gaccagaata ctgtgaacca tgctgcaggg
     4861 tatctgcaga tatcccttct gttatatccg gccggggtaa aatcagaaca ctgactgtca
     4921 ggctgcttca ttttctgcgt attccagact ctgcaggtac tgcagcgccg ccgggtcagc
     4981 cacatattcc atctgcccgt ggtgacgctc cacttcatta atgattttct caagcttcac
     5041 gcggaaaaat tctttacgca ggttaatcct gttaatgcgg tatttttcca gcgcaggcgc
     5101 atcatcacag gaaatcatgg cgtgaacatc aaaatcaaac ggaacagaag caccacttaa
     5161 ctctttgacc ctgtccatgg gttccagccg tcgggtcatg ccaattttaa agacatcctc
     5221 accaaacgaa ccaatattcg aaatgatata aacatgcccc tgtttcgtaa gctgagccat
     5281 ggatttagca cgttcatact gcgcatgcgc ttcctgaatt ttctgttcca gctccaggcg
     5341 ttgtttctct aactcctccc tgtgagcccc ttctgctgcg cgcagcgcct cctcaatcag
     5401 tttctgctgc tcagcaagac gccgttcttc ctcttccgct tcccgctggc gccggtcaag
     5461 ttcatcctga cgctcttttt cttcacgtat ctgacgctta agctctgcct gatattcctt
     5521 ctcttcctgc gcttttccag caatgccact ttgtccagaa actgttgcct gacactctcc
     5581 cagtcagaat tatcttccag gttaagaaag gctattttct cactgacagc agcgtaagcc
     5641 tctctttcct tacgaagctg gtcatatttg ctttgccagt tattaatcgt tacaccagcc
     5701 agcagagtct tcgccttaaa tttccaggcg gaatcaacaa tccgttgcat tgcacggctg
     5761 gccttctgtc tggcggtatg aaattcctca cgacgtctgg ccagttgctc cttttcctgt
     5821 cgtattcccc gggctatcac ggcattgaca gtttcattaa aaccccgctc tctctgaacc
     5881 agctctgaaa cggcctgctc aaaggctgct gttttctttc tgagtctccg gtaaataaaa
     5941 aaacgaagta aaattgcaaa tacagtaagc aaaccaaaaa acaaaccaat aatacctgcc
     6001 ggattcgata ctgcatcaat aaagcgctca ttaagattta tctgcgccct gaccgccaca
     6061 taaatccccc agagaataac gcaaactaaa atcaggacaa atcccgctgc attatcatct
     6121 tttttcttcc tgccatgact ttcctttatt ttccttctca gtaactgaat atctttgatt
     6181 aacgccctcc ggaaatattt atacccggaa ggctgtccag ctttatgata cccgccagta
     6241 cagaattaac tgcaggctga gcacagattt ctctgctatt tcagtgtcag attaatactg
     6301 attaccggaa caaatgggca gatgtgtaaa tccaggtttg ctggccatat aaagcggcgc
     6361 acaggtgggt ttacggggtt cagtgaccgg tacaacctca tttttcccgg ccaccgacgg
     6421 ttgtgacacc ttccggacag ccgctgattt tttcttctga accagcttcg tggtgaccgg
     6481 tgatttcacc tgacgtttgg cgggtgccac gccataaccg gaacaaaagc gcttcgactg
     6541 actcagactt ccgtcattgc agacaaaacg tccgtctgac gtgcagtgtg aaataccacc
     6601 tttagcaccc gagcagggtt ttcttccccg cgcagcatct gtctgaaccg aaaataatct
     6661 atggtcgccc ccgtttttgc aacactgatt ttttaattaa tggtgggctt gcttaaatct
     6721 atccggcgtc tctgtaggca aggatgcctg cgccccgatg agtttcgcac ctgataatcc
     6781 taaaaagccc ctcggcttca gtgagccatt ttatttatta ggtttttaat caggtcgatt
     6841 tgccgttcat gtcatcaata atcagtcttc gcaaaaccag acaggcaact ttgtatctta
     6901 ttgtttacaa taagccaatt gagttatatc agcatttata tcaagcctga tattcttttt
     6961 gctgagtagt gattacccag gcaattcgag ccagtttatt tgccagagcg caggcaacca
     7021 cattcgaatg cttcttgttg agttgctccc gaacccattc ggcaagtttt ccatgctggc
     7081 gttcaagccg catcatgaat gagcgggcac actgaaccag taaccggcgc aaatttttat
     7141 cgcctcgctt actgataccc aacagagtca attttcctcc cgtgctatac tgccggggaa
     7201 cgagccctgt ggaagccgca aaatctctgc tgcatgaaaa ctgtttcccg tcaccaggtt
     7261 gcgacgatag taagctggca gtaaccggcc caacaccagg aattgtcata atgcgctgag
     7321 cggtatcatc agcgttgatt gactgactta attctgattc cagctctgct atttgctcaa
     7381 caaggtaaag gtagtgggta tgcaacctca ccaaaaggcg gctcagatat tcggggattt
     7441 cgtgttcagc caacacgaga gacaaccgtt ttattacagc atcacctgtc ggcaggctga
     7501 ttccaaattc cagcaagaaa ccgtggatct ggttggttgt tttcacctta tctctgatga
     7561 gtgattctcg gacccggtgt aacgccctca tggcctgcag gggctcagtt cttggctgga
     7621 caaaacgcat tgatggtcgt gaagccgctt cgcatatggc ttctgcatca acaaaatcat
     7681 tcttattact tttgacaaac gggcgaacga actgtggaga aattagctta gtttcatgcc
     7741 caaaatcaga gatacgacga gccataaagt gtgcacccgc acaggcttcc attacaacaa
     7801 ctgagggcgg acatgtcgcc agaaactgca tcagtttagt acgggtaaat tttttacgca
     7861 gaagagcctt accagacttg tcttgacaat gaatgtggaa ggagtgcttt ccgagatcga
     7921 taccaataag tgtgatgttc tgcatgatgg tctctccgtt aaagggattc accttaacag
     7981 cgtactgctg ttaaggtggg ggtgaccatc tcattagccc cacgaacaga aaacagataa
     8041 ttttcttcat ttaaaaactc atacattgtt attgctgata tgccagagtt atcaggatga
     8101 tgaaacattc cgccagcgtt gtcataagcc ggaataatgt cacattcaca cctgacatat
     8161 atccatcact gtttatttct gttctgccct ccctgctccg gctcacgtct gaacgtaccg
     8221 ctgctgtttc agatggcgca acaacaccct gccctccggt ataaatgttg ttccgtaacg
     8281 aaccagcccg gcatctttca gttctgcatc aatgacataa tgcaactcac caggctgagt
     8341 ctgctcctgc atcgtcacgt tgacacgctt aatacgtggc tcatacttca gcagaacatc
     8401 tgaaagaaca cgcatcagtt gatgcgccgt tcccggcatt ccctgcagaa cggttgtcat
     8461 atccggcaga ccataatccg gcagatgttt aaggcttccg gcccgggtat tcagaatacg
     8521 ctgcatgtta tcgagcaccg aaagaataac ctgctcttcc gcactgacct ggtttaactc
     8581 cagcccgcca gcgaaattgc catagagaat ttcataaagg gaaggacgcg gcatcactat
     8641 ttatccttca ccggtaacag cgtcagggta ttaccggata cttcaataag acggggcgtg
     8701 tccggctcaa gctcatcccg gctcagcacc acccgccagg tatttttctt ccggtcaggc
     8761 tccagaaaca ttgctgccac accggtaaat ttcgccgcat catccagagg catatccacg
     8821 gttactgagc cacctggctg catccataca tctttctgtg caatgatatc gcctgccaga
     8881 atttcactgt cccccgtaaa aagcgcctga taatccgcgc tgtcaaaatt ccggttgtct
     8941 ttaagctgat aaatccgcac cacaaccgac aggggaatac cggcagcact ggtgttaatg
     9001 gcctccctgg cacgaatatc aaggtgaagt gtttttatct gtcggtaaaa cagggatttc
     9061 gtggcagata ccgtaccgtc tgccactctt tgcgtcaggc cacaccctga taacgacagg
     9121 gaaaacaacg caataatcag accgtaacca gccttaccag cgataatcgc cattttcctg
     9181 agcctcctta cgttgataat gttcctgaac ccgctgataa cgccccagac ggatggtcat
     9241 tacgcgtgcc gctgaagtcg ttatctttgc ctgtgttctc attacagccg tgcgccccag
     9301 ttgcggacta ccggctgccg gtcggcaact gagccggaca tccggcaaca gtgaacgctc
     9361 aacacaaagt tgcagacgta catccagacg gcttcccaga taaacgtgca acaacgccag
     9421 caaatcagaa tacagctgcc cgcccggtaa ccagccctgt acctcgctgc ccgtctgtgt
     9481 tgaaagctgt aataacacct gtccgttcac atccgtagca tgatcgccca tcaccgggcg
     9541 actcttcaga ctgaccggat gatgaacacg catggccagc ggtgttttca gcgggatccg
     9601 tcgtcggtca tgatgccaga cacgggcctg tgttccggga gccagcagcg ttaccagtga
     9661 tgtcagccct tcagctgttc ttccgggtaa caacatcagg ggcaataacg ccagaaaacg
     9721 cgataccggc gtggcaatat tctgtgcaca tccggggatc cccagtctgg ccagaccgag
     9781 cagatactgg gatgttttat cctgcccccc cgcctcaaag gtggccgggt acgaatattt
     9841 gcgccagata cggtaatact gggtgatcag acgatgactg aatatatcga gaaaatccgc
     9901 tgcggcttca tggccttccc gctgctgtgc aatatcactg atatagtgag ccggcaacgg
     9961 agacgtgacc ccgtaaagtc ccataaacgt cacatgtaca gtgggtggca gatgggattc
    10021 ttcaggaatt acagcatctt ttatttcact cgcaggaaac cccattcccg ggtatggaca
    10081 aaagcgaacc gcttcctgac gggcctgcca gccactgcca atgggaggct gatctggctg
    10141 gctttgttcc agtaactgac agaagcggta aaaattaata tacggaagct gtttccggta
    10201 acgggtaatt aaccgggcag gcgcggattg tgattttctt tccaccggat gcattttcct
    10261 tcaggctgaa caatcagtgt gagctgattg aactgattca tatcggcgta cagggccagg
    10321 aaacggttga gcatttcgcc aaacaggtga atatctccct ctccggcaaa cccgttgcca
    10381 tcaagagtta cctccacatc aagcccccgg agcagaaaac ccttttcaaa acgctgaata
    10441 cgatgatgct gtacagccag aatggcatca agccgacggt gattaagttc atcgtcccgc
    10501 cagttataaa gcgccagcgt gccacgcagc acttccgcac tgctcagcat gttaagaaat
    10561 cctgtcccca gatgactcat cacccgccag tgaaaacgat cttctgtcgg cggataaacc
    10621 ggcagcgtgg gtttacaaag attatgtaca ctgaccgggg tctgcagtac ctgttcacac
    10681 cgatcaagca gcgtactctg caacgcacga cggggaagct gaccattggt acctgtaata
    10741 cgtaatgaaa gcgtttcccg ctccggcatc cgatccacct cccaccgctg accgccaagg
    10801 ataagccatg tgtcatacat ccccgtgaca ccccgcttca cccgggtatg ataataacgt
    10861 tcgggtgcat catggcgcag cattccccca cgatgacgga agctggtaaa aggcacgtac
    10921 tctgcactgc cggttcgccc ggagcccgcc accgcatcca ctgaatatat ctccgtatat
    10981 ccatcctgca gacgcttcgg gcgcagcaga tattcgctct ccagcccatt gatgatgagt
    11041 ggatcggctt caagcgtaaa aaggttaatc accggcacac aatgcagaca cagggcatca
    11101 tctgtcacag gtaaatctgc cggccaggac tgactcagca ccacctcaat atcaaatccg
    11161 gaaatacctg ccggcaaaga aatattctcc agtccgttca gatgaacaaa cataaatttt
    11221 tcccgaaagg tgaaatattc cagcaataac tgatacccac tgaaagcact gtcacctttc
    11281 ggccacagcc gatcttcttc tgcaaaacca ccgggtgaaa accatccgtc aatgcggatc
    11341 cgctcatcat ttcccggcag cctcagatac agcgcagcca gacgctttgt catcatcaga
    11401 tgaagggtgc tgctgaccgg ggcttctgct gccagataca ggctcagacg atgcaaatcg
    11461 acctgagacc agtcagccag ttcactgcag gcaaaaccta ttttcagcac tgagcgcccg
    11521 tccggctccg tcgtcataac gaccttctcc actgccagag gattcagtgg tatggcccgg
    11581 gtggtacggt aacgacagac tgtatttccg gggccaacag ggcgggaagt cacttccagt
    11641 ccggctggta cggtttcagc cattttcatg acggataacc ggggagtcag tgccaccacc
    11701 gacagggacg ggatagtacg caggtaatga ggccagagca tactgaccag actttcagta
    11761 agctccggca aatcatcatc gattttctgg cgcagccgtc ccatggaaaa ggcgaaacct
    11821 tcaaacaggc gttccacgca gggatcaggc gtacccgctt tatcgagatc cagcatcgcc
    11881 gcccgatcgg gatgagcctg tgcaaacgcc tttccggctt cacgcagata acgcatttca
    11941 gcgtcaaaat aacgcagggt taaatcgtcc attatcttct cagaataaag ctaactgttc
    12001 cggagactcg cgctctgact tcagccatat ttcaaacaat cgtccgaact gaactgcagg
    12061 caggatttca ttaatcagat tacacatgaa tataattcag acatctcatg aacttcccga
    12121 attgcacaac acagcagctc tggcagcatc aatggcgatg agcccggcaa gaagatgttc
    12181 catatcaggc tgcagacgca ccctgtcact ttcagttttt gcggacttca tacgtaacag
    12241 tttcaggcgg cgggctttta cctcaaatac cagttcaggt tcccattgtg acagggtcag
    12301 ccgggttgca cgttcatcca gctccacaag cagatgcagg gcaaggtcat tttttccagt
    12361 ctgttcggca acacgggcca tcaacaggcg cagtagccag ttgctgcggt cagactgaat
    12421 accgggacgt gcctgtaacc agcttaaagc ggcttctgtt ccttcattat ccgcaatctg
    12481 cagggcttcc ggctcaagag ataaaatgtc gttatctccg ggggctgccg ttatcgttac
    12541 agaaggttct tccgcccagc gttcaccaca ctccatcacc tgctgctgta tccagctcag
    12601 cgtcacgtca tcggcaaacg gcatgccgtc gttaaaagcc agtgtctcaa gccccggcag
    12661 gcgcagaagc aatcctttca ggtcatactg aataatggca gcataatttt cttttccggt
    12721 ctgcaacagc gcctgatgaa tataccactg caggtccagc cacagatgac tggctccccg
    12781 ggcaaacata tcgtcacact gttcaagcag tgacaaccag ttctgctgca gatacaaccg
    12841 tttcagtgac gcacgccggt ccggccccgg tggggcaata cgggtccgtc catcagcaga
    12901 taacggcggt aactgatgca gcgtgtcatg ccggacgctt ttcatcagtc gatgagccgc
    12961 cagccagccc tcaggctggt cacgcagata ttttgccaga acacgggcct gtgacagcag
    13021 ttcctgtccg gaggtgatgg cagataatgc catgacgctg ccggatgtta ccgtcggtgc
    13081 cggcgcggca gcctcctgag gcaccactgc atcaacacca ccggccttca tcaggcgatt
    13141 ttcaagtgcc tgatataacc cgttcagcac cggggcggaa gcctcatccg taaaggtctg
    13201 ttctgccagc cacaacaccc cggcaatgcg cagggtatcc gccttcacca cttccggata
    13261 cagagacaga ctgtccagta tgcgggcact gcataaccac tccagcgccg ccttcctggc
    13321 ccgtgcccgt tgtggatgca ggtgctctcc aaaacgctgc aacaaaccgg ccagcagctc
    13381 cagaccgtca gccagccctt tttcaccatc ctgccgcaga cgggcccaga tataccaggt
    13441 cgccacccgg atatctttgg ttctggtggt cagtaatttt tctgcaagct ggctgacaat
    13501 tccggcatcg gctccggaga gtttattgac ctcttcacgc atctgctgaa aatcatcatc
    13561 gtaccccgca tcatcacctg cgggattatc tggcgtcacg ggagcaagcc atttatccca
    13621 cagcgcaata cgcacctgag ccggttcagc cacatcccgg ggtgtcgtct ggcatgcact
    13681 gaggagcgaa tgaatggaag ccatcagtca gtctcctcca cggtatcccc ggcatcttcg
    13741 tcattgcctg taaacgctga cgtgccggag agttcgaaaa ccgtttccgg cagaacaaaa
    13801 ttgcggagtt tcagcaatac aagcggccct tcccccgctt cagttcgcag tgtgtaattc
    13861 agcatacgac cgtcctgcgc ctgccagctc aggctccagc cactggcaac gcccggtgcg
    13921 gctttccgtc gggccatctc aagcagacgt atcagccccc agcttcccgg caggtctgca
    13981 tactgacggg taccggcctg agtgcttacc cagcttaaac tggcgccagg tgcttccgta
    14041 tcagccggcc aggtaaatcg cttccatacc ggcatctgat taacataaat cagtttctga
    14101 ttatcggtta tcagcgtcgt ctgcatcaca ccggcagcag ttcccgggcg cagctcaaaa
    14161 tgcagccccg cgttacctgt ggtgaacgcc acatccgcta tttcactcag ggtattgatg
    14221 gctttcagaa acgcagggtt gaatgtcagt ccccgggtat taatggtatc cggtacccag
    14281 cgactgcctt ccctgtgcaa cacaccactc aggttattct gcaggaaacg ggcaatacgt
    14341 cccgtgtccg tattcaggta ttttgccagc aggggaagcg aagcatcgct gctgacattt
    14401 ttaaacggat aacgcccgga aaatgcatta ttccagccat ccaccaccgc agtacgccac
    14461 cgggcattca gactttctgc cgcgggcgta agaacctgct gccacgcctg ctccatcggg
    14521 cgaacaaaga ccgtctgtcc gaagccatac cactcctgtc cgagtccggc agcggtcagg
    14581 ctgccgtaat cccgggtatc ggttaaatca accgatttcc cctgcaatac tgtctgtgcc
    14641 agcatctgca tcatggcctg cgggtcagaa gagccggcaa tctgttgcag acgcagacga
    14701 acctgtgtca cccgggtcag atatgtctgc agattcagca tatccgcact gttgttctga
    14761 ttatccatca gtgcaagcac cggcccgaaa gtcgtcgcca gaggaccgtg aagccggctt
    14821 tccggtactg ccactggctg tttttcctgt gaaagcaggt ttctggcaga tttaaccagt
    14881 gaatcagtaa ccgcttcccg gggctggcca gtacgcccct gaaccgccag cgtattcatc
    14941 agagcaacca ggggggactg gcgcacatca gccataagcg tcagttgctc tgtcacatca
    15001 gacagcatct gagctttacg caagtgcaga ctgttcagaa aattcagcca ggcattacca
    15061 aaatcagcaa aatagcgcgt tgtcaggcgc tgtctgagtg cttccggcga taccggtgac
    15121 ggtgccttct gcctgccatc cgtcagtacc cagtccattt cttcacgacg ctcattaatc
    15181 acggtgtcta tcgacggcaa tacggcctct tcccaggcct ggcgggtaaa cataccggga
    15241 accacttcat ccgtaaaaaa gaggcgggag acatcagtat caccggtcat atccgtcaga
    15301 cgcatatccg caaactgatg tgccacccgt gccagcattt tctgatacag catggcatcg
    15361 ctgttctgtg ttcccagatg gcgaagcagc agagttctgc tctggctgac cagctcttca
    15421 tcagccgtca gcttccactg aggatgagta ataatcccgg aggcataata cgccagcaac
    15481 gtcgggccat tagcctgcca gaaaacggca ctcacaccgt cacgctgtgg ccattcccgc
    15541 atgagcgtac gactgaacca ggcgggctcc atatgttgcg gttgcgccag catcagatac
    15601 agtctgagct ggtcatatgc ggcttttgcc attttccccc gctgcgggct gtccggagga
    15661 agacgaataa atgtcctgag ctgctgctca agatgagcag cagcggcatc acgcagtaaa
    15721 agatgtgcat tttgtgcata aaggggcatt acgaccgcga gcaggtctgc attctgattc
    15781 ataccggccc gcaggtacca tggcgccccg tgctcctccc ggtactgaag gcgggccagg
    15841 gatttctgca gattcagcaa cgcctgcaga cgttctgcag gagacagttg ctggttctga
    15901 gccggacgaa tctgttcctg tacttcagca accagactcc ggtttgccag aaaggaaacc
    15961 accattcctg ttccctgagc catcatcagt atggccgcca taacggccag acttctgcgc
    16021 cagttatgac ctgtgcggga aggctgtagc cggacaggta attgctgcac tgtctccggc
    16081 aacgcctccc agcggttatc cattcgccag cgatgtctga cacttcgttc accgccgaca
    16141 gttgccggac tgaaaacaat acccgccagc aacaactgac ggtatggacg gagtaatacg
    16201 gataatggct ccaccacagc atcaatattt cgtctgaatc gctctgccag cgacaacaaa
    16261 aaataatacc gaggggcaca gcatatctgc tgtatcccct gagcaacaag cccggggagc
    16321 atggcctgta gttgtgcttt cagtttgtca gaactacatt ctgctggcag aaggcatccc
    16381 acgggctgag taatacgccc tcgctcatcc ggactctcct gcagtgacca gacatacagc
    16441 ggcagacgcc agccaaggcg ctcacaacat gaactgaccg cacgggaaac cctgtcaagg
    16501 gcatcttctg tcagtggtgc ggataatgtt tcactcagtc cggaagttac ccagacaatg
    16561 ccatcagcag gccggtaacg cagacggcgt aacgcagcca gccaatctgc gttttccggc
    16621 tgcgagggct ctcctcccca gagaagcaat gtcccctgct cttcctgcca gaactgttct
    16681 gtcaggcccg gggtcagcaa ctcaacctca gaggcagtac ctgttatcaa cagaatgcgt
    16741 attttacgtc cccagaatct gccatactgg cgccgcagag tatgacgaat atcctgaaca
    16801 gtaacatagc gcggtggcgt ctgagccagt cgctcttcat cgtcaggtaa cctttgctcc
    16861 ttacgggccc gacgtcgata tgtcagttcc tgaacatatt ttttcatcac aggcaggtaa
    16921 cagacaaaga gcaaccccag actcagcaat atcagcagta gtatttgatc ccggcgtgtt
    16981 tctgccccca gaaatgctcc atatcgggtt atcagccacc agagcgcagc cacaccaata
    17041 aatacaatcc ccggtcgtcc aaagcgacca gacagacagg ccagtttatt cattctggtt
    17101 tcctgatgta acagtactga taaaatgttc ttctccggat gagaataaag tcaggagaga
    17161 atcattacca agacagacca gatcggctgc cagagcagta agcagccacg gagcagcagg
    17221 tcccgggata ccgcaccatt tttccagtaa catacgtccg gacggttccc atccggcacc
    17281 atactggttt ccaatagtca tcagcggtgc agcaatcttt tcccagtgat aacagtcccc
    17341 cagtacacga gcggtacgac aggcagctgt ctgggtttca aggaaaagtg tgaattcatc
    17401 attaaacttg ttgatatcca gcgacatcgg gcgtagcaga cgggctggat gcggcaggtt
    17461 atatttttgc gcaacatcat cgctggtcaa caaaagtgca gccagtccgt cagagtatgc
    17521 atttccccca ttaagctgta ttaccagtat caaatccaca gtcagcactg gttgtttcag
    17581 acgctcatca acccacccca tggaaaagtc gggagtaacc tcgatattgt caggaacgac
    17641 gcgttgggga aataacgctt cccatgcaga aacaaaagca gatctgattt cagaatcagg
    17701 ctgatcggtt atcagcgtca catttacagg gagaacggca ggtaactgac aaaatttatc
    17761 cgttatctcc ctaagcaaga cacgtaaaga ggcttcaacc ttatgcccac tgtcagaaag
    17821 ataatctatt ttctttacaa ggccgtactg cagaggcaat tcatcacaaa gagtagctac
    17881 cgttatttta tcaggcaaat aaacacaact ggcagtaata acaagatacc tttcggccca
    17941 ggattcccac tgtttctgag catactcccc ctctttttta agaaacaaat gttcatccat
    18001 ctggttgctc cactgccagt gtcgcaggca aaacaggaaa aaccagcccc ccggaggagt
    18061 caatgcaagc caccagatac tgaaatcatt taatgctttc acatattctg atgcccggat
    18121 aataaacaac agagcaccaa caacagccat aaatgcccct gtcataaacc acaacatcat
    18181 tgatggttta tgaggtaatt ctgttgataa tgattttgtt ctgctccatc ccatgggtta
    18241 tcctattgca cattccggtg cactggatat aaccttacac ccacacagac atttacatcc
    18301 atcaatcaca acagctcttc catcagatat ccattcagga gagccctcca caataggatt
    18361 agtaccatgt ccggaaacag gacaactaac catatctccg acaagagcta cttttttacc
    18421 atcgacaatc atggttgaag atgccgaaat aactttacca ccatgtgtgg tattatcacc
    18481 aagcaataca aaacctttag acataaaact ctcaaaaata aattttactt tgagtcacca
    18541 gttgccgtag gggtaaattt tccctttgat aaaatctggt gtatcaccgt aataaacata
    18601 tccatctgga tgtttagtaa cccccttaca catattcatt ttatcacccg atactgtagt
    18661 aattttagca tccaactgag gtaccaccgg attaccgcga tctcccagcc agactcttac
    18721 cttcccttca ggtgccagac caataatcat cgttgaacga taataggttt cgttccagcg
    18781 gttgtctgtg taaggcgtaa ccatctgctc ccaggtccct tttttaaaaa aaatcagagt
    18841 ttcataactc tttttatcaa ttattgaatc ccagcaaaaa cgcaacatca gaggtaatgc
    18901 tttccccttg ttataatacg actcagtcgc gcccagatgg tcactccagc gccctacact
    18961 gacctgactc ggctcggtat tatcaagata ctgataagtc gtcttataac cttccccgtc
    19021 caccatcaat gcctgagtgg ccagcgcagg cagagccttt ggataaataa agagaaattt
    19081 ccattcatca tagggtaatt ttttataacc ccattcagtc ttatccgaat cagcatactg
    19141 tgagctaaca gaattacacc cagttaacaa tgccaataat ggtgcgataa aaatgatttt
    19201 atctctggtc atgtattgtc atcctgtagc gttaaacacc agacatcata actttagtat
    19261 tttttactgc gttatcattc aatcgctgct gatcatccat gtaccataca gaacgaatcc
    19321 agcgatcatt tggtttattc ggaaaaacga acgccttatt acctttagga ccaaagcgat
    19381 ggataaaaat ctctcccgtt gtaggatcca cccataaatt accatcagac tcaatattcc
    19441 agttggcaga acaatgggtg tattttccga taatatacaa ctcttcctca gtaaaagcct
    19501 gaggctcttt ccccaaacgt accgcttttc cctgagcaat ggctttatca acaaaaggaa
    19561 ttaattcagg agataaattc atatctggat ttttggggtc aattggagca aacaataccc
    19621 ctgcttcctg agcagcatcc agcatcacac gcaggcaaac attagcccag tcattaggta
    19681 ctgccatacg ctcaaaaaat actgcggctc ccacctgctt ttgtatcacg ccagcccgac
    19741 gttgattgct gttgttcaca cctacggaac gaattttggt ggttagtttt ccatgaggca
    19801 aaatatgttt taaggctggc agagtcatca acatttttct ggcttgctct gcattacggt
    19861 atactcgcgt atctgtttct ctggtatcgt cactgacaat ttcaaatgcc gggcaactca
    19921 gaaacagact ttcattttcc tgcagaggtg aacctaccgg attgtaacca cctccaatat
    19981 ctgaatgcgc ccctggtaaa gccagttcgg gccacatacc tttaatactg ttcaggctga
    20041 aattatatcg gtattcattc attgcagtaa tctggaaaac tttcttcgcg atggaaggac
    20101 gcaattccag ctttactccc gggttgctac gtccattaat atcgaagaaa ttactgattc
    20161 caccaatagc tgcgacagta tcaaaaagcc ccagaaaacg gacttcaccc gaaggtttac
    20221 catcataaaa atcgcctctg agccctttag caatagctct ggctatagca ggatcctgtt
    20281 ccataacccg attagcaaag tgccttgccg ccgctgctcc ccggctaaaa ccaaaaacat
    20341 caaactggat ttttgcgata caaaaatcag gatttaaatt gaaccccata aattctgata
    20401 acgcctgggt gattcgttcc attgcctcgt cggttttcgt caccacacct tcaaatatat
    20461 ccagaatact ggtgcctaat cccatgccaa tgactgaatc tgcttcacca gccgcagtcc
    20521 ctatcccgga aatgtaagtc ttaatttgag ctgacgtctg gtctttttta agtacctgat
    20581 ctggatgata gagtatatta agccagtgaa tattggaata atatccacga taactgccat
    20641 tcgagatatt gttcttgtta atttcttcaa gaaaatcctc acagctttgt gcatcttttt
    20701 gatttacccc ctgtctggct gcactacatt cattaaattt catcagacga gatgctgtat
    20761 tctcccgatt gtttccggta ccatcgaaaa atataccaat ggttaaagtc acaccccgcg
    20821 ccggtttttt ctttgcaaca acaactttcc agtcattatg aatactggtt ttatgggtgg
    20881 catctgttac gtcatatccc tgatattttg tcattctgtt tctccatgaa tttttacctt
    20941 cccaaactca acttttacta ctgacggaat ggaggtgttg actggcgctg ttttaccatt
    21001 ttcatcggtg atcccttgta gaatatcacc ttcagccgtt gttaatcgat aaggcgtcga
    21061 aggttgcggc actcccgctt catcggtaag aataaaaccg ccaccaaacc ctcgcggcat
    21121 ttcaacagac gcaatatcaa gacttgttgg tcccatcttc tgcatattcg tgctttttaa
    21181 caggatctgt cccgggcatc ccagctcaat atttccaccg cttagtttga tataagctcc
    21241 accacatgcc agtatcagtt cctgattagc agaaacagtc actttgccct cagaactttg
    21301 aatatcaaga ttctgttggg aactcattga caaatcgtca ctttgagcct gaatatttac
    21361 ccgccctttt gctgctaaca gttgcatccc ctggtttctg ctcaacaggc taacagcacc
    21421 ttctgctaca actgtaaatg actgcccggc actaatatca gtgttatctc ccgacattat
    21481 gcccacgctt tcactacccg aagcaattcg taacgcccgt gcactaacca tgccaatccc
    21541 atcaggagca tgcaacagca ttcccggcgc agtgagatct gttagcgatg aattcaaccg
    21601 ttgctgacaa cttatatctc cctgagtagc atttgcagca gttgcggcct tcgccatact
    21661 tcgggccaga gataatgcct gctccagttg gcggatagct tcattcatat ccagcatctt
    21721 tccttgtgcc tgaggctgca tatctgcact gataaatatt ccttttcctc cccggatact
    21781 tatccagtca tcagtcctca gctcagcgcc ttctcccctg agtttccggg aggcattaac
    21841 attatgacca agattaagtt gtgtcttacc gccaaactcg gtactgatct tgatatgctc
    21901 ctcaccgcgt ttgtcctcca tccgcaactt gttatttgcc ggcgtacgga taacattgcg
    21961 cgtaccgttc ttatcggtaa cgtgatcaac atgacgtgaa tcatgcaggg catgcgcgat
    22021 ataggggcga tccggatcac cttcctcaaa ggcaatagcc acttccgttc cctgaatcag
    22081 cggaaaatga aaaccataag catcaccact gtagggtttg gccagacgta ccggcatgct
    22141 ttcctgtccc ggatttttat catcacggtc agcatcaaat ttcacccggt acatgcctga
    22201 tgcatcctgc caggcgtaga tatccccttc ttttgcactg gtcacacggg ccgtcatggt
    22261 accggtgatt ttcggacgag gaagtaacgg tggtcgccag cagataactt cactgtaagg
    22321 aacgcccttc agtacaacct gcagggcatc cttgcgactt gcagaaaaac cgacacaggt
    22381 aagcaacacc ggattgcgca gcagcgcagg caacgtcggc ggaagactgt cggtgatagt
    22441 cagaacctga gcaggcgcca gcgtggcgtc tgtgctgttg ccggttatcc gtacctgctc
    22501 cgccagataa cgttcatgac caaggcgggc ataaaaattg gccgtctccg gcaccgggtc
    22561 gattttatcc ccccgttcca gatgacggaa atggtagtgg tacacctcac catagttaat
    22621 tccgtcacca tcaccatgcg tcatgtctgc cggggcggac tgcaacagat attgtgcttc
    22681 acgatggtta taagcctttg tggtgacact ggcctctgcc acattgtgcg tcacattcag
    22741 cccccacact gaatccgctc tgttatcatt cattccggac ggactgttta tcgccagcgt
    22801 cttatcaaac gtcagcgcag cctggctgtc accaaaatgc actatttccg tctgcgtgtc
    22861 cggttgcagg gtgaaaaaat aaaaaatccc cacttcagcc agcaaacgct gaataaattg
    22921 caggtcactt tcctgatact ggttaatttg ttcacgcttc ggatacgttt ttttgagacg
    22981 gaactcaaac tcccagcctt tcaggccgtg ctcctggaga acctctgtca ccacctccgg
    23041 caccgatttg ttaacgaaaa atcggtgaga acgatgctgc ctgtccagta gtttaataaa
    23101 tggttccagt atcacctggt attttgcctg gtcggcggag cccgatatac gacgaaaatc
    23161 tgtaatgaca ccatgtacta ctttctggct cacctgctcc ttaagccctc ccgtacccat
    23221 tgtaaatgtg gctgatttac gcagcagcag cccggcatcc aagtcgttgt cagtactggt
    23281 aaaattgatg atgtaataat aggtctgact catctcctcc ctgccgctga aatgctcaac
    23341 atccagtggt gacgggcagg agggaataat taaatggtaa cgattcaggg aagcagagat
    23401 agtttttgaa agtgtatctg tcagactcat attgttaact ccatgtctgt accggtattg
    23461 aactgcaatt atataatgat ggccgttaag ctttattttc agagagaatt actgataatc
    23521 atcccattca tcgtaaatat atgcatatgc aaatcgatac atcagataac cgacaaaaaa
    23581 taacgcaaca gagagccctc cccacagata tttaaattca gcagatgaga gaaaaaagaa
    23641 ccagactgta aatacaatac ccgacgcacc taaccccata cctgcgccac ggataaatcg
    23701 ggaaagcatt ataagtaatg ttctttttat cttcataaac caccgaaaaa atattcgcat
    23761 atccggtcaa actcagccga atttcggtct gcgacaatac aggctcgttc aaacgggcgc
    23821 acaatatctt caacaagaaa gtacaaaaga tccaaatccc cctgagcttt cagcttatca
    23881 tgcaacaccg gatttctgtc tttcagaaaa cgggatgtgt aaatcgcaca cgatgtctct
    23941 gcgccaacag ataataacaa cccggcaaaa ctccctgcaa aaaaagcagc cagccctctg
    24001 gataccagaa cctctcccag tttaaccccg gtcaattcag caaatgccat tctacctgcc
    24061 agatctcctg aaacgttctt cacaatggag cctgtttttt ccatattgat ccgggataca
    24121 aagtcttcaa gacaaatact gataattttc ccaataatat cacggctgaa aatgcctttt
    24181 tctaccagtc tggccattct gaatttgtca ttctggtttt cccgcctatg cccggtatct
    24241 ataaaatcat atcccatata accgagactg actggcaaag atatgagtcc cttcccgacg
    24301 gcccccagaa acccatcccc attaatattt acggcctcca gcatatcccg ggcaatttct
    24361 ttagcactgt ccatatatgc tttcctgcgg ataatcgcta ttgattccat caaacgctat
    24421 cacaatacca tcctcatcat gccacgtcag agacaggtac gtcggatgct gtctggcagc
    24481 aagatagctg agcaactgcc ggctcagtac cggcagcagc tgctggttca gcagactctc
    24541 cacattccgg gcgcccgtat ccggcagcag gcaggcggat gtcagggcat cgtacacacg
    24601 ttcatcaacc gatgtgctca gcccataatg gcagtgcagg cgttcacaga cctgtgcaag
    24661 cttcatctcc acaatgatgc gcatggcaga ctgcgtcagc ggacggtaaa tcacggtccg
    24721 gaaacgggca agcagtgctg gctggaagtg gtcgcgcagc agtggacgga taagctcatg
    24781 cagctccgat tccgttgtct ccggcttttc gctcagttgc tgcatcagaa ggtcactgcc
    24841 cagattggag gtcatcagaa tgaccgtatt acggaagtca atttcacgcc cctcaccgtc
    24901 gcgcataaag ccccggtcaa acacctggta gaacagattc agcacatccc ggtgggcctt
    24961 ttccacttca tccagcagca ccacactgta cggacgctta cgtacagctt ccgtcagaat
    25021 acccccctga ccgtatccca cgtacccggg cggtgaaccc ttaagctggg aaaccgtatg
    25081 gggttcctgg tattccgaca gattgatggt aatcagtgat ttttcgccgc cgtacatcac
    25141 atctgccagc gccagtgccg tttcggtttt tcccacacca ctgggaccga ccagcaggaa
    25201 cacgccctgg gggccgttac cggatgtcag acccgttttc gccgcacgca gacgctgcgc
    25261 aatggatccc agtgccacgt cctgtccgac cacccgtctg ccgatatcct tttccagatg
    25321 cagcagttcc gtctgttcgt ccttcatcag ggaagataac ggcaccccgg tccagtccgc
    25381 gaccacaccg gcaacggtgc gtacgtcaac atccacactg agcaggggct gattctgctg
    25441 aatgtcgtgc agtgcctgct gcagatgcag tgtctcactc tgacgggaaa tatcgctgcg
    25501 actttcaagt aactcaccgg tgagttcgca ctcccgggcg tactgctgct ccaggtcatc
    25561 acgtgctgtc atcagcacat tctcttcctg ctcaatggca gacagacgtt cgccgtgatt
    25621 ctgatgacca acggcaatat cttccagcag tgcctgtttt tccataccga gggaagcgag
    25681 ctgtgaacgg atacgggtca gctgttccgg caccgtgtcg aggctcatac gcacacgggc
    25741 agcagcagta tcgagcaggt caacggcctt gtccggtaac tggcgttctg tcagataacg
    25801 gcgcgacagc gtgacggcag cacgtaccgc ctcatcggtg atatgcacgt tatgatgttc
    25861 cgcataacgg gatttcaggg agcgcagcat cagacaggcc gtctcatcat ccggctcgtc
    25921 cactctgacc atctgaaaac gccgctccag agccgcgtca cgttccaggt attgtttgta
    25981 ctcagaccag gtggtggcgg caatggtacg cagttcaccc cgcgccagcg caggcttcag
    26041 caggttggcc gcatccgcgc cgccggcctg attaccggcc ccgataaggg tatgggcttc
    26101 atctataaac aacagtacag gagccggtga ctgctgcacg gcatcaatca cgtttttcag
    26161 gcgctgttca aattcaccct tcacgccggc accggcctgc agcaggccga ggtcaagggt
    26221 gcgcagggtg acgggtctga gtgattccgg tacgttacct tccgcaatac gcagggcgag
    26281 cccttccacc agagccgttt tccccacacc agggtccccc accagaatcg ggttgttctt
    26341 gcggcggcgg gagagaatat ccaccatctg acggatttcc gtatcgcgtc ccgataccgg
    26401 gtcaattttc ccctctctcg cgctggccgt gatgtcccgg gtgaatttat ccagcgcact
    26461 ctgtaacaca ggcggcaggt cacctgacgg tatacccgcc gtatccgcac cgactggacg
    26521 cccgataaag gtcacatttt ctccctgagg cagcacaggc tctgcatcct gtaatctctc
    26581 cggacactca tcagactgcg catccagcag cggggacaga cgctgcaact ggctctgact
    26641 cagtgtcagc aggggccaca ccccgtcaca gcgaaccagg tgcggatttt ccgtcagggc
    26701 acccagcaga tgcacgctgc ggatatgttc ttccccctgt aatgaggcca ccatccaggc
    26761 ctgtttcagc agggcattca gggactgcgc aagctgcggg cggctgcgta cgctacgggg
    26821 ctggttatcc agccagccga gcagcgactg ccatatcgcg tccatatccc agtcataacg
    26881 ccggcccagc acggtaaggt ctccttcccc ctgctccagc agtttcagta accagtgctc
    26941 cggggtgatt tctgcatggg cccgggtctg gcaaagggag gctgcgcctt ccagtgcacg
    27001 ggcacagtaa tgattaagac gacgtaacag ggctgccgga ttttccatgt tattcactca
    27061 ctgtcagaag accttcagac acgctaccgc ccttcgccat aacccgggcg catcaggtct
    27121 gtgttcagga ttgagaattc tttcttcagc acccgccaac agatactgaa gaaaaaagaa
    27181 aatgacggac aggataccct gcccgtccgg gagaattacg cagtggtacg ttcattccac
    27241 gcgtcagaat gaatgatgtt accgtcctta taagtccagg tgattttctc gtagcgcagc
    27301 tccacctgct caaggtggtt atgcttctcg taggcgggat tcttgatgtc gtgcatgacc
    27361 gggttaacct tcaccacctt cacgttctca agcttcgtat tgaaatactc cacttcctga
    27421 ccggcatcat tgattctgta ccacttaaat tcagcagact tcagggtctg ccccgtggtc
    27481 accgccttgt aaaggtaggg gctggaggaa tcaatttcct tggtaaacag gaacggcgtg
    27541 tgaatacggg taccggtcag cttaccggta ttgttatccg tcggaatgta aaggcagtgc
    27601 tcctgagcca ccacttcaat actgccctca cggtccttca catccacgga gcctttaata
    27661 tccgctccgc catcatcctg cagccacaga taaacaggaa ttgccataaa gttactccct
    27721 ctcgttgtct tacgcgccct gttacggcgc ccgggtgatg tccggcagac gacaggcatc
    27781 ggcctgcggt accagactga tttcaacacg gcggttacgc gctcgccctt ccgccgtctc
    27841 attcggagcc acgggacgac tctcaccata tccctgtacg gcaaaacagc tttgagggat
    27901 atcgcccgta tcccgcatcc agtcgcgaac ggcctctgcg cgcttcagtg acagggcctg
    27961 gttgcgctgc gggtcgcccg tattgtccgt gtgaccactg accacaatca gccagcccgc
    28021 ttttgcctta atatccacca gggcattcac cagccatttt gtggcgcccg gtttcagcgt
    28081 ccatttaccg gcatcaaaca gcgccaggct gttaaggcga accattttcg gcgtctgagt
    28141 aactttctgt attaccggac ggggaggcag tggtgcaatc cactcaccag tcgctcgctc
    28201 aaccggcggt atcagccaca ttccctgata caatcccagt ccgtaacgct gcggcacacc
    28261 acgttgctgc cagtcgttca gtaactggct gtcagcctga agacgttgca gtgcagtttt
    28321 tttcgattca gatgaatatc cgcgcatccg gtgccaggcc gtcaggtgtt cccccacgct
    28381 gcgaaccagt cgctggttat tcataaaaga cgccagcata gccagcagaa gaaaaacgcc
    28441 gacaatcgcc cccgtctgga gacaaagtct gtgccagtga ctgagtccac gatgacgggg
    28501 aataccggac agcagaatat ccggcaacgg cagtgcctgt gatgacacgc cggcaggcgg
    28561 tggcagtaca gtgagtgacg tcagttgttg ttgccagaga ttaccgggcc gggtcgcaac
    28621 cgacgtcaga cagaatccaa cggcaaccgg ccggaaataa aagtgctcct gccggcaaat
    28681 atctgtcagg gcatcagtaa cccggttgta ccaggacaac atggcattca gccataacag
    28741 ttcactgacg caccggagcc tggacgctgc atcacactcc cggagccatt cctctgccgg
    28801 aatggctccg gcttccgtca ggcgtatgtg catttccgac aggtccggtg tcagtgtaaa
    28861 ccagtgtgca gagtgcttat ttaaggatgt gttttgtggt gacggccctg aaaccatcaa
    28921 tgtcaccagc accggcggca tggtattcag ccactgccgg cactgcatga ctgcacgctg
    28981 ccagttccgc aaccacgaca ggaaagcttc atctgaggta tgctgctccg gtaaaagtcc
    29041 cagcatgacc acgacctggc cacacagggc cggacggctg tacgacaacc gctctgccag
    29101 tattctcatc tgctccggcg tatccacacg aagataccat cccgatgctg tttcccggta
    29161 tgcctgaccg ggttcgaacc acgcatctga gtttccggca acaagcaaca catgcccctg
    29221 aaaatactca ggcggcagac cgtcatcgcc ggcattctcc gggatatcac gacgggaaga
    29281 cagacaacgc cactgatacc acagcacaag ccccgccgcc acaactgaaa ccagggtcag
    29341 tattatctgg tttactgcag acagtggcag gaatcccgaa atcagccaca gtgtcaggaa
    29401 cagactcagt attgtcagcc agagacggga acgtacgtgc atggttatcc ctgcccggta
    29461 agccgtgcca cctggtctgc gagcacagaa gaaagcacac accagagacc agccagcgtc
    29521 acaatacccg ccacccatga caaccagtac atcgttctgc caacacggta acccggtgca
    29581 cgcaccacca ccggcgcatc ctgtgcgaaa gtaaatgcag gaacacgcgc tgtcagtgcg
    29641 cgaattacat cttcgcgacg ctcatcatcc tgcgaccggt actgaccggt aaaaccgagc
    29701 tgaagcgtac ggcagaggca tgtcagtacc gccacatccg gcgcaggtaa tttcagttgc
    29761 tcacgaatac gctcccagag ctcttctccg gcattgagcg taccaaaaaa atgagcctgc
    29821 agcgggtcct gctgccaggt gcgccagcca tcgtctgttt tttcacggtt cagcacactc
    29881 tcgtccagca gggcgcagaa ggcatacaac atgatttcac tgctttcctg actgaattct
    29941 gcttcagcca gctcttcacg cgcctgcgtc accagctgac aggcacggcg atacagggcc
    30001 ttaccatcct caaccggttg tccgcagcgc agctggctga ccatcagcca gccgggataa
    30061 aaaatctgtt cagcccggga gataacaggt ttattcatgt ccgcagtacc gcaaaaagtt
    30121 ccagtttcac atcccccagc gatgccgggg tgtagaaagt acaacgcccc gcgtccagca
    30181 tcgcccgggc tgcatccgta cttaaatcca gagcgaaata ctggttttcc agccggagcg
    30241 gaatggccgc cggtacatgc gtaaccggac ggataatgac cccgctcagc gccacattca
    30301 ccacctcaga cacatcatcg gggcttccgg ctttgcatag ctgcggaaac ttcgtctgca
    30361 gttcatgccc gggtatggaa gagcgcactg aaagccagaa atccgcccct tcgcgcaaac
    30421 gcgcatcatg taatgccccc tcccacatca caccattttg tttcagttca atgaaaacca
    30481 cacgggaagg caggcttgcc tccagcagct ggttcagcaa agaaagcaag ggtggaaaca
    30541 cattttcagg tgtttcatga cggtatgccg gaaccgcatc cacattatgc tcaagcgaga
    30601 atgtgagcag ggagcctgcc agccgggcaa gctccctgta caacagttcc gggtggcggt
    30661 aaggcatgtc aagcaactct ttcagaacag gctccgcact gttcagggca ttcagcaacc
    30721 agaacaacga gacgtccgcc actgcaaaat ccgcaagacg ggcattgttc tcccgacgca
    30781 tggacatcag tcgctgacga cgcgcctgca ggtgatgaag caactccgca agctcagtca
    30841 tcagcgaagg actggcagac agcgtcagca gagggggaat aaaacgcggg tccctgcacc
    30901 actgtccctg tgcatcacga acaagacggg taacaggaca ggtcagccag gcagcatttt
    30961 cctgatgagc catgcgcaga gtcaggttat gccgcaatac cgccacttcg ctttgctcat
    31021 gcccggccag ctcctgcaca ttcacccttt ctgatttcca gcgacgcgga cgctcgctct
    31081 cactgccatc atcaaggttg ccaccgttag cattcagtaa cggtagcgcc agcacaatat
    31141 ccaccaggga acgctcagaa accgtcgata aatcacagac cggaggcaga ttatccgcac
    31201 gttccgtatc aataagcgtt ccgtccggga aacgaacaat cagacgggtg gcattcaacc
    31261 gtgaaagcgg taacagagag tcatcaaact ctgcagccac cacgccccag ggatgcgcca
    31321 gccccattcg ggcaacgctg tctgccagat gaacatccca ggcagcctgc tgctgaaact
    31381 gctggggcat cagaaaagcc ccgtcttccc acagtggacg ataaatcttc atttcccgac
    31441 gcctgcctta cgcttttgcc ttcggcatct gcgaaaccag tgacagactg acgtccatac
    31501 cttccacctg gaaatgtggt acagcataga gtttcacgcg gaaaaatccc gggttgtctt
    31561 cgatgtcttc caccaccact tttccgtcac gcagcggatg tgaggcctgc agttcatcac
    31621 ccggatcggt catctccgtc accagacccc ggatccagtt attcagttcc agttcaagca
    31681 gacggcgatc tttggtggta ccgatattct ccctctgaat gatcttcagg taatgcgcaa
    31741 tgcgggacag caggaagata tagggaagac gggcattaat acggctgttc gccgttgcat
    31801 ccggcgtatc gtacagtgcc ggtttctggg tggagttagc ggagaagaag caggcataat
    31861 cccggttctt gtagtaagag agcggaataa agcccagatt agcgaattca aactcccgag
    31921 tttccggaat catcacctct gacggaattt tgacctgatt accggttccc agatcataca
    31981 gatggatcgg caaatcctgc accgcaccgc cggcctgagg accgcggatc tgaacacacc
    32041 agccgttggt gacaaaactg cgcaccatat tggccgcaaa agcaaaagac gcatttgtcc
    32101 agagatactt atgatgatcc ggacctttca cctcttcaac atagttaaag ctgcggacag
    32161 gtacggtatc aggaccatat ggcaggcgtc ccagaacacg tggcatcacc agaccaatat
    32221 agcgggcatc atccgtttca cggaaagcat tccacttgat gtactcggca cgctcaaagt
    32281 gattgccaat atccttaatg gcggccacct cttccatcgt atccttccgg aagaaagccg
    32341 ggccaactga accaataaat ggcatatgcg cggacgcagc cacccgggaa atatttttca
    32401 gcagcgccac atcctgcgca gacgcatcaa actcccaggc agaaatcagg gctgcaatgg
    32461 gttcaccacc cggcgtatcg tactccgcca cataagtctg cagataaaga ccactctgaa
    32521 taatttccgg cgcatcctca aaatcctgac gcagatcgtc tttggacaga tccagcagtt
    32581 caattttcac attacggcgg aagtccgttt tatccaccag agacttcacg ccccgccaca
    32641 gggactccac tttctgaaat tccggatgat gaagcacggc atccagctga cggctgatct
    32701 gataatcaag ctctgcaata tgatggtcaa tcagcgcctt atcgagtttc tccaccggtg
    32761 ctcccgcttt cgccaggcat tccataaaca cctgcatccc tgcggtcaga cgttcatctg
    32821 ctgatgcctc tgacatggcc tgcggatcct gccagatatc cagcgcactc agggctgata
    32881 ccggattcag attaattttt tcaaacaggg aagcatatac gctgtcgccg gaatgcgttt
    32941 cagaaaccgc atttcccgcc acattatttt ctttctgtac agacatcagc atgctctctc
    33001 ttaatccgtt aatcacacct gttccggcac cagtgcactc agttcgtcac gtaaggcctg
    33061 actcagttcg ggatcccgca gaattttttc cagttcttta cggaaagtgg cgttatccag
    33121 cagattcgcc ttcagatccc gcagcaaact tcgcatggcc agcatggctt taagacgggg
    33181 aatttgtcgg gcaacctgct ccggcgtgaa atcgttcata ttacggaaag tcaggctgat
    33241 gctctcttca gtaccatcac cggcaagtgt gttctccaca ttcaggcgta ctgacggcga
    33301 gatatcagcc agaacagcat taaagttatt tttattaaca tccattttta tgcgctctga
    33361 cagcgggcgc tcttcctgtc cgttactgaa atcaccggca accagcagtt tgagcggaag
    33421 ctccactttc ttctgcccac cacctgtatg taggtctaat ttcagattca cccttgcctt
    33481 cgggatctca ttctgaaacg aactgctcat gacgttctcc tgtgaaacct gcatcccagg
    33541 gagcccatcg ccacagaagg cctccccttg tgcactgatt taatttcacc cattatagga
    33601 cgaagatctg caaattctca atatgacaga catatcgatc gttttaatgc caataaccag
    33661 atctttattc tgatacatat cgcaaaatca gcatttatca aaacttatat ccaggggtaa
    33721 tacaaaaatg cagaaaatta ttccggttag tgcttttgat acagatggtt ataccgaatg
    33781 attaccagca ctgacattca cgaggagtga tgattcggca gcatttaagg gcatatcaga
    33841 aaaagagaag gacatgaaag aggagagcaa taaaagacac ggaaaataaa gaagagagag
    33901 aaaatatttt ttaaaaacag aaaattaaca acgcacatca ggaaacgtcg tctcctgatt
    33961 cagtgatacc acatgaaaat ttagtgaacg gttaccgttc tgaagtctat gtcagaagat
    34021 attccattac caaaaatcaa tcagcgatac agagacgatc atggcgcgct tgtcaccgtg
    34081 accagtgttg aggaaacccg gtggtgttta tgcgggacgg ctatccgcat ccctgcatgc
    34141 ggccgatgta caacttcctg ggaaaattca aacccgaacc ccggaaagaa ccccctgccg
    34201 gcaatcacac tgcctgatgg cagcataatc cacttattaa ttctcatccc cggagacaag
    34261 cccagaaaac cactccgggt gttcactgta tgccgtgttc agcgcatccc aggaaacgtt
    34321 gatataaaag ctgtcctgca atttgcgggt aacaatttct gccacccggt tacggttatc
    34381 cgcctgtccg gcataacctc tctctgccag ccactcattc agttcatgtt ccggagacag
    34441 attgataata tcgtaatcgg accttgccat tcccgtttcc ttagcacatg ttttcaggct
    34501 cggggataaa gaatgtcgta ctgctgctgc cagagcaata ttccccctga tggtcgaatc
    34561 agagatagta ggccggtact ttgcgggttg ttcccgtcag attatcatgc acctccgcgg
    34621 tcgttgtcac caccggcatt ccgctctttc tcaacctcag gacatcagcc gcaatacgct
    34681 gactgccgaa ccagaacagg ccgtccagtg cagtcaccag caaccccgcc tccagcgcat
    34741 gcttcagccg ttcacggggc gctttcactt cccgggcaat ctgctggtat ggcgatgatg
    34801 tgttttcatt cccaatcacc cggcgaatac gatgagacag atgataccgg tatgtatccg
    34861 gcacaccgga aaggctggcc ttcaggctgt acacgcagcc aaagcgttta tcgttgaaca
    34921 ccacattttt ctggctgata tcccattctt cacgcagcgc ggcaatcagt tgtggtgtcc
    34981 gggtaagcaa caagcgaaaa ggcagttcaa aactggtgac gtaatccaca ttcagcagtg
    35041 caatacgcag ccgttcttcc ggcccggcct ctgtctgccg gcactcctcc agaacatcct
    35101 gccactgctg gtgaaggcgt ggaagttcat tcagttcagt aaaactgtat ttatccgcaa
    35161 gatagtcaat tcgtgtacgc atactgaaaa gaattccgta taaagattca gctggcaaaa
    35221 ctttatcagt ctgtaaaaac taacggaaga gtcgatattt ctcccgtcca tcaccggatg
    35281 attgttgcaa taccccgtgg catcagagac tgaacagccg tttttaacgc cacgtattgc
    35341 cctgatgaac caggccgggc aacccgaaaa catctttcca cccggcattg tccgccagca
    35401 cttatcaccg accaggtctg ttgcagtaaa tccaccagtt gcgcacattc ttcatcagcc
    35461 atgacgccga ctcgcggatg gcaaatactc gtctggctga gcagcaacag acatcgtatc
    35521 tgtgctcctt catgttgttc ccgcaaccag cgtaatacca ccggcgctga tgggctgacc
    35581 gtgtgattat gctccgtaat acgttcgtgt acccgtcggt gaaaggaact acaaaatgtc
    35641 tgaatctgtt gaccatagat tccttctgcc gaatagagcg tgaagtgaaa tgccaacaga
    35701 tttggccagt ggtccaccgc ctgctgaaca aaacgccgga tttcccccgg ctctgaaatt
    35761 aaggcttcgg ttatttgcac gaaatgttcc ttttcaaccc ctgttgaatt ttgtcaagcc
    35821 ggtgcagaca cagtaaagcg cgcatcaaca cctgaaaggt acgatacaaa cacaagttac
    35881 aaacaataag aacggaggac aattaatcag tgaggaataa taactaaatt aaccaaaaga
    35941 atgttactga caatactacc aatacttaaa tagcaatata ttaattaatg taagaacaga
    36001 tgcccggtaa gacatataca caatacatcc acaaacacac tcttttcatt aaatcaatca
    36061 cctgattaaa ccatgaaagt gtgatgtaag tcaaaattac tgacactatc aattgataat
    36121 gattatctct atcattatcg tgaaattcga gaatgaaagg agaagtagtc attataagcc
    36181 ttttataaat cgtcatcatc atagtaaagt agaacacttt ttatatgagt tactatatga
    36241 aaatatactg gacaagaaaa cgcattcctg aactttcagc acttccacct tcattaagaa
    36301 agaaaaattt tactgacgca tataatgcag catcttctca tatcgaatac tggataggtg
    36361 ctggcgtctc atttatctcg atgatgattt tatccagagt atatgacttt cttttacccg
    36421 cccaagatac tttcccgggt gatatcatca gaagtttatg tgttgtgtgt ccatccatat
    36481 taatctggtt tcagttttcg gtatatgtca tgaggaaata ttacagacat attctggtgc
    36541 gaggaaaaga aaccgaaacc atttcagaaa ggttgatacg tgaggctgat acacgagaat
    36601 atgaactatg gcgtccggtc cgtcgctttt tctccattat ttttctcatt attctattag
    36661 tttgcattca ttcactgata acaactataa aataaaagtt atggctgttc tgatacagga
    36721 aaataaacgt tcattctatg gttacctctt cctgttatat agaagaattt taaacgacaa
    36781 gtaagggaat ataaatggaa ataacaaaaa ataaaaatgc aactataaaa aaacagtcat
    36841 cagtcggctg cttttcaatg ttt
//
LOCUS       DAZGXZ010000037        36780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000037 DAZGXZ010000000
VERSION     DAZGXZ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.037"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            140..382
                     /locus_tag="ACHKJF_001950"
     CDS             140..382
                     /locus_tag="ACHKJF_001950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949311.1"
                     /translation="MMMARDITAYHLNSVHISARRFPGIFQWATGMQTKNLPLTNSLL
                     QNDYAESIFVFNGERDVSEMPLPEEMLLTGNAESER"
     gene            complement(1308..2984)
                     /locus_tag="ACHKJF_001951"
     CDS             complement(1308..2984)
                     /locus_tag="ACHKJF_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0949312.1"
                     /translation="MSEVKKHVSYSLTVAAMLSAAIVCQTAHADVNTPNITGEKNNTT
                     TRFSGNIYGSENTVTSESNSLVVGNSNSVEGGYNNIVIGNSSTVKASGLIRKVGEDIV
                     LDGYSVSLGNATRINGDSSVAIGLTSSVTGNDSVALGSHSSANGNNIVSVGSDTLKRR
                     ITNLAQGKDDHDAVNLMQMTSAVNREAGERIKSTNNLQNQLNTLSGQVITETRARTEG
                     DAAALAAARAHSDENDKRTLVSANTYADQKETAINNRTDGLLTTERQARTAGDTETLA
                     AARTHSDENDKRTLVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLAAARTHS
                     DENDKRTLVSANTYADQKETDINNRTDGLLTTERQARTAGDTATLAAARTHSDENNKR
                     TLISANTYADQKEIAINNRTDGLLMTERQARIAGDAETLATAEAYSDAGDSLTLKQAT
                     AYSDISTRRALSEANRYTDNKFRNLSNKIERAEKRLNAGIAGVTAISSIPYVAENSFS
                     YGIGVGNYQNANAVAAGIQYKTSRNTNIRLNISWDSSHNTAIGAGVAGGW"
     gene            3216..4706
                     /locus_tag="ACHKJF_001952"
     CDS             3216..4706
                     /locus_tag="ACHKJF_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP0949313.1"
                     /translation="MSFNPTDEQYAIINWQGQQLVVNAFSGTGKTSTLVQFARASPDS
                     RMLYLAYNRAIRDEAERKFPFNVECRTSHQLAYARVGRHYRHRLVPGLRITDVARKLN
                     TRYWALARVAGTGLNHFICSADAVPGLHHLPDKDEMRGVPPADALRAVQLLWNEMSNP
                     GGTFPVTHDTYLKLFQLSGADLSHRWDTILFDEAQDASPVTTALVLGQKCRVVLVGDC
                     YQQIYRFRGANNALSHPALKNADRLWLTQSFRFGPAVARMANLLLQREGETREVKGCG
                     GDDEVLLKCHAREHLQGHYTVLSRTVAGVIATALMAAMKGQKVYWVGGIEGYRTGELE
                     DLYWFQVDMPERMHSDRLRRDYRNFEEYKYIAKSTKDVEMNQSLRLLELCFPLPKKLE
                     LLRQYTVTNEQDADITVSTAHRSKGLEWPVVMLDEDFQDITDPLMTEAERHDETCLLY
                     VAVTRALRTLVLNDLMHQLAEEQQKTEGYRGTGYDISGLVQENGHA"
     gene            4699..6180
                     /locus_tag="ACHKJF_001953"
     CDS             4699..6180
                     /locus_tag="ACHKJF_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFP0949314.1"
                     /translation="MLERLKRLFPVKTECSDVRTDIPGETVPEGYFTPLPADVLVSTP
                     FRKRCLKQLWENSSMPVDMYQRLCLDPVHQLLSVSQNLPATAEGHWGYTGGFGDLTLQ
                     FTTYAVRLARGRMFPPGATAEEQAAQGSVWNMVVFWSALFYHLPLLAELEGEQADGRC
                     WQPGISVPEGVYRFRFRKMPCQGTEGRQLAALMAGQLLPGEAGAWLATVPGALQNLAG
                     AVWHQHPEMALIRTVLQEAMEEVVSPFAAGDVAEVPVAEVVPVLLQSAAISAESVIQP
                     LPVVPSPVEDAENEGRTAEQEVDEGNETDADTGMLLTLFSAADLSDEGGQIASGVPPD
                     KESSIELPEESGCVTENQGNRIPETVEKTAEFSLTEDSRVESEFIRWLKEGLREGSLS
                     VNSVDSNVHVVSGYVFLCAPGIFYRFLKESGSGQDRRYVQSLFERAGIHKVRSGERFF
                     QARLYSDAEHSGRYKMVSGYLVKGRFLYGNITVPGDSPLIVFG"
     gene            6248..7285
                     /locus_tag="ACHKJF_001954"
     CDS             6248..7285
                     /locus_tag="ACHKJF_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0949315.1"
                     /translation="MQNDDSVTKITWEQLLEEYFFSKNLRPDTEWSYCKVVRVFREFI
                     GDILPGEVTVRDVQRYRRYVLKEQKRREVTWNNKVAHLRAIMGFGIKMKLLPQTENPL
                     VEASVASTEKKKKTLSDNQMLRVYLVVQRFAEAEEQNMVNSRRRNAIYPAWYWLTVLD
                     VLRYTGMRFNQLKHIRVRDVCPDESIIDLKLEGSKTHREWGVPLVAPLKVSMEKLLER
                     ARQCGAGDDDYLFDVCRFSEPISADRYQYCPVRAHQTVRSFFRRLSRECGFPVSPHRF
                     RHTLATRLMKYPERNLHLVKSILGHRSLTTTMGYVDVCMESAAQTLEDALSLYTDERT
                     VSKICKGGNKS"
     gene            complement(7481..7556)
                     /locus_tag="ACHKJF_001955"
     tRNA            complement(7481..7556)
                     /locus_tag="ACHKJF_001955"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7521..7523),aa:Phe,seq:gaa)
     gene            complement(7663..8238)
                     /locus_tag="ACHKJF_001956"
     CDS             complement(7663..8238)
                     /locus_tag="ACHKJF_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0949316.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(8275..9972)
                     /gene="dsbD"
                     /locus_tag="ACHKJF_001957"
     CDS             complement(8275..9972)
                     /gene="dsbD"
                     /locus_tag="ACHKJF_001957"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP0949317.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAAPQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLKAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(9948..10286)
                     /gene="cutA"
                     /locus_tag="ACHKJF_001958"
     CDS             complement(9948..10286)
                     /gene="cutA"
                     /locus_tag="ACHKJF_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP0949318.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(10402..11703)
                     /gene="dcuA"
                     /locus_tag="ACHKJF_001959"
     CDS             complement(10402..11703)
                     /gene="dcuA"
                     /locus_tag="ACHKJF_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP0949319.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(11821..13257)
                     /gene="aspA"
                     /locus_tag="ACHKJF_001960"
     CDS             complement(11821..13257)
                     /gene="aspA"
                     /locus_tag="ACHKJF_001960"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP0949320.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            13594..14070
                     /locus_tag="ACHKJF_001961"
     CDS             13594..14070
                     /locus_tag="ACHKJF_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP0949321.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(14086..15342)
                     /gene="yjeH"
                     /locus_tag="ACHKJF_001962"
     CDS             complement(14086..15342)
                     /gene="yjeH"
                     /locus_tag="ACHKJF_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP0949322.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            15618..15911
                     /locus_tag="ACHKJF_001963"
     CDS             15618..15911
                     /locus_tag="ACHKJF_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP0949323.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            15955..17601
                     /gene="groL"
                     /locus_tag="ACHKJF_001964"
     CDS             15955..17601
                     /gene="groL"
                     /locus_tag="ACHKJF_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP0949324.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            17739..18092
                     /locus_tag="ACHKJF_001965"
     CDS             17739..18092
                     /locus_tag="ACHKJF_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP0949325.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(18295..19164)
                     /locus_tag="ACHKJF_001966"
     CDS             complement(18295..19164)
                     /locus_tag="ACHKJF_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0949326.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSQRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(19554..20582)
                     /gene="epmB"
                     /locus_tag="ACHKJF_001967"
     CDS             complement(19554..20582)
                     /gene="epmB"
                     /locus_tag="ACHKJF_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0949327.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVVSGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            20624..21190
                     /gene="efp"
                     /locus_tag="ACHKJF_001968"
     CDS             20624..21190
                     /gene="efp"
                     /locus_tag="ACHKJF_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0949328.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            21242..21367
                     /gene="ecnA"
                     /locus_tag="ACHKJF_001969"
     CDS             21242..21367
                     /gene="ecnA"
                     /locus_tag="ACHKJF_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0949329.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            21478..21624
                     /gene="ecnB"
                     /locus_tag="ACHKJF_001970"
     CDS             21478..21624
                     /gene="ecnB"
                     /locus_tag="ACHKJF_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0949330.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            21806..22123
                     /gene="sugE"
                     /locus_tag="ACHKJF_001971"
     CDS             21806..22123
                     /gene="sugE"
                     /locus_tag="ACHKJF_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0949331.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(22120..22653)
                     /gene="blc"
                     /locus_tag="ACHKJF_001972"
     CDS             complement(22120..22653)
                     /gene="blc"
                     /locus_tag="ACHKJF_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0949332.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(22742..23875)
                     /locus_tag="ACHKJF_001973"
     CDS             complement(22742..23875)
                     /locus_tag="ACHKJF_001973"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0949333.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(23938..24297)
                     /gene="frdD"
                     /locus_tag="ACHKJF_001974"
     CDS             complement(23938..24297)
                     /gene="frdD"
                     /locus_tag="ACHKJF_001974"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0949334.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(24308..24703)
                     /gene="frdC"
                     /locus_tag="ACHKJF_001975"
     CDS             complement(24308..24703)
                     /gene="frdC"
                     /locus_tag="ACHKJF_001975"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0949335.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(24714..25448)
                     /gene="frdB"
                     /locus_tag="ACHKJF_001976"
     CDS             complement(24714..25448)
                     /gene="frdB"
                     /locus_tag="ACHKJF_001976"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0949336.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(25441..27249)
                     /gene="frdA"
                     /locus_tag="ACHKJF_001977"
     CDS             complement(25441..27249)
                     /gene="frdA"
                     /locus_tag="ACHKJF_001977"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0949337.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            27574..28551
                     /gene="epmA"
                     /locus_tag="ACHKJF_001978"
     CDS             27574..28551
                     /gene="epmA"
                     /locus_tag="ACHKJF_001978"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0949338.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            28770..30272
                     /gene="yjeM"
                     /locus_tag="ACHKJF_001979"
     CDS             28770..30272
                     /gene="yjeM"
                     /locus_tag="ACHKJF_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0949339.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(30423..33746)
                     /gene="mscM"
                     /locus_tag="ACHKJF_001980"
     CDS             complement(30423..33746)
                     /gene="mscM"
                     /locus_tag="ACHKJF_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0949340.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(33768..34736)
                     /gene="asd"
                     /locus_tag="ACHKJF_001981"
     CDS             complement(33768..34736)
                     /gene="asd"
                     /locus_tag="ACHKJF_001981"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0949341.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGAFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(34833..35885)
                     /gene="rsgA"
                     /locus_tag="ACHKJF_001982"
     CDS             complement(34833..35885)
                     /gene="rsgA"
                     /locus_tag="ACHKJF_001982"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0949342.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            35980..36525
                     /gene="orn"
                     /locus_tag="ACHKJF_001983"
     CDS             35980..36525
                     /gene="orn"
                     /locus_tag="ACHKJF_001983"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0949343.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            complement(36538..>36780)
                     /locus_tag="ACHKJF_001984"
     CDS             complement(36538..>36780)
                     /locus_tag="ACHKJF_001984"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949344.1"
                     /translation="PDLGKVVLYQLSYSRVLLNFAFVIFAFLRRYGRRIIREIFPSAT
                     PQKSFFCVFISSAEIISKTFNLASKSRRRKWIPALT"
BASE COUNT         8778 a         9037 c         9771 g         9194 t
ORIGIN      
        1 tgaaataaat aatgtggtat gataataaat ccaggtcagc agtaactggc tatcgtgtat
       61 aattatccag aagtcctgct gagccttccg tctgttcatc atgtgccagg tttgcgttcg
      121 ggatatgatg tgccatctta tgatgatggc aagagatatt actgcgtacc acctcaattc
      181 tgtacacatc agtgcccgtc gtttccccgg gatttttcag tgggccaccg gtatgcaaac
      241 aaaaaatctg ccgttaacta attctttgtt acagaatgat tacgcagaaa gtatttttgt
      301 atttaatggc gaacgtgacg tttcggaaat gcctttaccg gaagaaatgc tactgactgg
      361 aaacgctgag tcagaaagat aattcggtgt attcatgact gttatcaatg ttttatgtgt
      421 atgatgtcaa cgacggatga aaagttatcc acttatatga ggcattatag gattatttct
      481 ggctgacgtt gtttcagttc agaccagggg cagtgaatat gctgacctgc tttttcgctg
      541 atatcagtcc ccctgtaatt acatagataa tagatatacc tctccgttga tcaggctgtc
      601 caggtaattg ataccgcact tcctcatcag ttcgtagcag agtgtactta gcatggctag
      661 tgcagtttct aatgatttac agtgtgtatt caggagagta agaaattgct caaaacatcc
      721 tggtttatac tggtgctttg atattcattg tgtccctttt ccgttcgtta ttatttccat
      781 atttaatatg atgcctgtac atgtaaaata atggaagatt tcaggggagc attgcataaa
      841 gtccgcctgg agaataaggc tcaggtcagc gaatcgcatc gtggccagac tgcactgatt
      901 gcaataagtg cggatacaga gttgcagcta ctcgtcgata tcagaacaac agaatccttg
      961 caaacaggga tgtgttcatt cagggggtat gatgctacga cgaacgcttt tagtgattat
     1021 taatcgacga tactgtgttt tgtctggatg gtgagggact cgctttgagg attcaccagg
     1081 gaaatacggg gcttgtggca ataatgttag cctgcgaact taaatgttta cgggcaacat
     1141 ggcggcagga aatctgccgc cggattgttg tgaaggctga ccacactgcg cgcccttttt
     1201 ccttgattat cccgctcagt ccagtggtct gttccggatg gggctgtgtt gcgtactgaa
     1261 ttaatgtgat tacggaatac cataatgccg tatgtacgca agattattta ccagccgccg
     1321 gcaacccctg caccaatcgc ggtgttatga gatgagtccc aggagatgtt gaggcgtata
     1381 ttggtattac gtgatgtttt atactgaatg cctgcggcca cagcattagc gttctgatag
     1441 ttacccacgc cgatcccgta tgaaaaagaa ttttcagcca cataggggat ggaggatatt
     1501 gctgtcactc cggcgatgcc tgcattaagt cttttttctg cccgttcaat tttattgctc
     1561 aggtttctga atttgttgtc ggtataacga ttggcctctg acagtgcccg gcgggtactg
     1621 atatcgctgt aggctgttgc ctgtttcagt gtcagtgaat ccccggcgtc actgtatgcc
     1681 tctgctgttg ccagagtttc tgcatcacct gcgatgcgtg cctggcgttc ggtcatcagt
     1741 aatccgtcag tacggttatt gatggcgatt tctttctggt cagcataggt attcgcactg
     1801 atcagtgttc ttttgttgtt ttcatcgctg tgcgttctgg cagcagccag agttgcagta
     1861 tcgcctgcgg tgcgtgcctg gcgttcggtc gtcagtaatc cgtcagtacg gttattgatg
     1921 tcggtttctt tctggtcagc ataagtattc gcactgacca gtgttctttt gtcgttttca
     1981 tcgctgtgcg ttctggcagc agccagagtt gcagtatcgc ctgcggtgcg tgcctggcgt
     2041 tcggtcgtca gtaatccgtc agtacggtta ttgatgtcgg tttctttctg gtcagcatag
     2101 gtattcgcac tgaccagtgt tcttttgtcg ttttcatcgc tgtgcgttct ggcagcagcc
     2161 agagtttcag tatcgcccgc ggtgcgtgcc tggcgttcgg tcgtcagtaa tccgtcagta
     2221 cggttattga tggcggtttc tttctggtca gcataggtat tcgcactgac cagtgttctt
     2281 ttgtcgtttt catcgctgtg ggctctggct gcagccagcg ctgctgcatc accttctgtt
     2341 cgggccctgg tttcagttat gacctgacca gagagggtat taagctgatt ctgcaggttg
     2401 tttgttgatt ttattctttc tccagcttcc cggttaacag cactggtcat ctgcatcaga
     2461 tttaccgcat catggtcatc tttcccctgt gccagatttg tgatgcggcg tttcagtgtg
     2521 tcagagccga cggatacgat attgtttccg ttagcactgg aatgactgcc cagtgccacg
     2581 gagtcgttcc cggttacgga cgatgtcaga cctatggcaa cagatgaatc accgttaatt
     2641 ctggtggcat taccgaggct gacgctgtaa ccatccagca caatatcttc gcccactttt
     2701 ctgatcaaac cggaagcttt gaccgtggag gaattaccaa taacaatgtt gttataccct
     2761 ccctccacgg aattactgtt acccacaacc agactgtttg actctgatgt gacagtattt
     2821 tcactgccgt aaatatttcc ggagaatcgt gttgttgtat tgtttttttc tccggtgatg
     2881 ttcggtgtgt tgacatcagc atgtgctgtc tgacagacga tggcggctga cagcatagct
     2941 gcaactgtta atgaataaga aacgtgtttt tttacttcgg acattatttt ttcctgccag
     3001 attatgacct ttcaggtccc ggtatcctgg ataagcgcgg cagacaggag accacctgcc
     3061 tttctgagga caggatgaga agcatccggt gtgatggcga tgattaattc atcatatgac
     3121 cagggaaatt tttctgttgc tgtacagagt tatttgtggt gggcataccc ctgatgcatc
     3181 agattacgga aatgtttttc cggagaagag aatacatgtc ttttaatccc acagatgaac
     3241 agtacgccat tattaactgg caggggcagc aactggttgt gaatgcgttt tccggcacag
     3301 ggaagacgag tacgctggta cagtttgccc gggccagtcc ggacagccgt atgctctatc
     3361 ttgcctataa ccgggcaata cgtgatgagg cagagcgtaa atttcccttt aacgtggaat
     3421 gcagaacgtc acatcagctg gcgtatgccc gggttggaag acactaccgt catcgtctgg
     3481 ttccgggatt acgtattact gatgtggcga gaaagctgaa tacacgatac tgggctctgg
     3541 cacgggtggc agggacgggg ctgaatcact ttatctgttc tgccgatgcg gtaccgggcc
     3601 tgcatcacct gccggataag gatgagatgc ggggtgttcc gccagcagat gcgcttcggg
     3661 cggtgcagtt gctctggaac gaaatgagca accccggggg gacgttcccg gtgacgcacg
     3721 atacttatct gaaactcttc cagttgtccg gtgcagacct gtctcaccga tgggacacca
     3781 tcctgtttga tgaggcgcag gatgcgagtc cggtgaccac tgctctggtt ctggggcaga
     3841 agtgtcgggt ggtccttgtt ggagattgtt atcaacagat ttaccgcttt cggggtgcga
     3901 ataatgcgct ttcccatcct gcactgaaga atgcggaccg gttgtggctg acacagagtt
     3961 tccgttttgg tccggcggtg gctcgtatgg caaatctgct tctgcagcgt gaaggtgaaa
     4021 cacgagaggt gaaaggttgt ggtggtgatg atgaggtatt gctgaaatgc catgcgcgtg
     4081 aacatctgca ggggcattac acggtactca gtcgtactgt cgcgggtgtg attgctactg
     4141 cgctgatggc ggcaatgaaa gggcaaaagg tttactgggt ggggggaatc gaaggttaca
     4201 ggaccgggga acttgaggat ctctactggt tccaggttga tatgccggaa cggatgcatt
     4261 cagacaggct gcgccgggac tacaggaatt ttgaagaata taaatatatt gcaaaatcca
     4321 cgaaggatgt ggagatgaat cagtcccttc ggcttctgga gttgtgtttt ccgttaccga
     4381 aaaaactgga acttctccgg cagtatacgg taacgaatga gcaggatgca gatatcacgg
     4441 tatcaacggc acaccggagc aaggggctgg agtggccggt tgtaatgctg gatgaggatt
     4501 tccaggatat cacggatccg ctgatgacgg aggccgagcg tcatgatgaa acctgtctgt
     4561 tatatgtggc tgtcacccgg gccctcagga cgctggtact gaatgacctg atgcaccaac
     4621 tggcagaaga acagcagaaa actgaaggtt accgggggac cgggtacgac atctcaggtc
     4681 tggttcagga gaatgggcat gcttgaacgc ctgaaaaggt tatttcctgt gaagacagaa
     4741 tgctctgatg tccggacgga tattcccggg gaaacggtgc cggaggggta ttttacacca
     4801 ttgccggctg atgtactggt atccacaccg ttcaggaaaa ggtgtctgaa acagttatgg
     4861 gaaaacagtt ccatgccggt ggatatgtat cagcgccttt gtcttgatcc tgtgcatcag
     4921 ttgttgtctg tcagtcagaa tctgccggcc acagcagaag gtcactgggg ttacaccggt
     4981 ggttttggtg acctgaccct gcagttcacg acgtatgctg tccggctggc cagaggcaga
     5041 atgtttccac cgggagcgac agcagaagag caggccgcgc aggggagtgt gtggaacatg
     5101 gtggtgttct ggtcggctct gttttatcac ctgccgttgc tggcggaact ggaaggcgaa
     5161 caggccgacg gcagatgctg gcagccgggg atatctgtac cggagggcgt ataccggttc
     5221 cggttcagga agatgccctg ccagggaacg gagggacggc aactggcagc cctgatggcg
     5281 ggacaactgc tgcccggaga ggcgggagcc tggcttgcca ctgtcccggg ggcattacag
     5341 aatcttgccg gagcagtctg gcatcagcat cctgaaatgg cgctgatacg tacagtatta
     5401 caggaagcta tggaggaagt cgtgagtccc ttcgctgcag gagatgtggc cgaagttccg
     5461 gtggctgagg ttgttccggt actgctgcag tcagcagcaa tttcagcaga aagcgtcatt
     5521 cagccgttgc cggttgtacc gtcgccagtg gaggatgccg aaaatgaggg ccggacagcg
     5581 gaacaggagg tggatgaggg gaacgaaact gatgctgata ccggaatgct ccttacgctg
     5641 ttttcggctg ctgatttgtc ggatgaggga gggcagatag cgtcaggcgt gccgccggat
     5701 aaagaaagca gtattgagtt accggaagag agcggatgtg taacagaaaa tcagggaaac
     5761 cgtatcccgg aaacagtgga aaaaacggca gagttttccc tgacagagga tagcagggta
     5821 gagagtgaat ttatcagatg gttaaaagag gggctgaggg aggggtcact ttcggttaac
     5881 agcgttgatt caaatgttca tgttgtttcc ggatatgttt ttctgtgtgc cccggggatt
     5941 ttttatcgtt ttctgaagga gagtggttcc gggcaggatc gtcgttatgt tcagtcgctc
     6001 tttgaacggg caggtatcca taaggtacgt tcaggagagc gtttttttca ggcccgtctt
     6061 tatagtgacg cagaacattc cggacgttac aaaatggttt cgggatatct ggttaagggc
     6121 aggtttctgt acgggaatat tacggttccc ggagacagcc cgcttattgt tttcgggtga
     6181 gtaaaggcga tgatgtgcag aatttgaccg gagagtgcgg gagcacagta aacaagggga
     6241 atatataatg cagaatgacg attccgtcac aaaaatcacc tgggaacaac tgctggaaga
     6301 gtactttttt tcaaagaatc ttcggccgga tacagagtgg agctactgca aagttgtcag
     6361 ggtattcagg gaatttatcg gtgatatttt gccgggggag gtgactgtcc gtgatgttca
     6421 gcgataccgg cgttatgtcc tgaaagagca gaagcgcagg gaggtgacct ggaataacaa
     6481 ggtggcccac ttgcgggcaa ttatgggatt cgggatcaag atgaaactgc tgcctcagac
     6541 agaaaacccg ttggtggaag catcagttgc gagtactgaa aaaaagaaaa agacactgag
     6601 tgacaatcag atgctcaggg tttacctggt tgtgcagagg tttgcagaag cggaagagca
     6661 gaacatggtg aatagtcgtc gacgcaatgc tatataccct gcctggtact ggctgacagt
     6721 tctggatgtt ctgcgttata ccggaatgcg ctttaatcaa ctgaagcata tccgtgtgag
     6781 ggatgtctgt ccggatgaaa gcataattga tcttaagctg gagggaagta aaactcacag
     6841 ggaatggggc gttcctctgg tggctcccct gaaggtatcg atggaaaaac tgctggagcg
     6901 ggcccgtcag tgcggggcag gtgatgatga ctatcttttt gacgtctgtc gtttcagtga
     6961 gccgatctct gccgacagat atcagtactg tcccgttcgg gcgcatcaga ccgtacgttc
     7021 atttttccgg cgactttcgc gagaatgtgg ttttcctgtt tcaccacatc gtttcagaca
     7081 tacgctggca accagactga tgaaatatcc tgaacgtaat ttacatctgg tcaaatccat
     7141 actgggacac aggagtctta caacgaccat ggggtatgtt gatgtctgta tggagtctgc
     7201 agcacagaca ctggaagatg ccctgagttt gtatacagat gagcgcacag tgagcaagat
     7261 ttgcaaaggg gggaataaat cttgacaggc tgaatcagcg atgtaagcat acagtgttaa
     7321 cgagaacagg gcgtctccgc aaaagacacc ctgtttgagg atacgatacc gaactcaatc
     7381 agtgtaacca caacttacac aaacggagac cctttgatgc tgcatcctgt attaaccgcc
     7441 cgtcaaggat ctcatccctt aaccagcgtg ttctggtata tggtgcccgg aggcggaatc
     7501 gaaccaccga cacggggatt ttcaatcccc tgctctaccg actgagctat ccgggcaacg
     7561 gggcgcatta aaccgtaatc tgcacatctc gtcaacctaa tttcagtaaa agcgattcaa
     7621 ctgcttaaga ttgcggcaaa tcgcttcttt tctgtgtttc tgtcaggtca atgcgccacc
     7681 ctggcggcag cgggcgaagc gcaggatatc ttccgccagc cgatgtgcag tgtcgacatc
     7741 cgcctggcta cgattcacca gcattcggct taaacagcct tccagcacca gttccatctg
     7801 ctttgctacc atcgccggat cgtcaacttc cagcgtggtt aacagttcgt gggtgaaatc
     7861 gtaggccgcg cttttttgct gatcggccag ttgatgaata gggtggccag gatcgggata
     7921 aaacgtacag gcagcgataa atagacagcc cggatagcgg ttgtttttaa cgcactccga
     7981 taacgcctga taacgtgcca gcagcttttg ttcggcggtt tgcgtttcgt ccagcatcag
     8041 ctgacgacgc cagacatcta tctgttggct aagataacgc agcgcatcgt agaggattgc
     8101 ctctttgtct ggccagaagc ggcgtagctc gtccagtgga taatccacac gttcagcaac
     8161 catctccagc gtggtgttgg caatcccttg taattctaat aatttcaggg cttctcccag
     8221 tacatcttca cgttgcacgc tattttcctc cgtctttccc actgcaagtg tcgttcacgg
     8281 ttggcgatcg cgcaaatgtg cgctgaaggt ttcagcatcc ataaagcccg tgacgcgtgc
     8341 ttgtggatgc tcctggcctt gtccgtcaaa aaagagaatt gtcggtaggc caaggacatt
     8401 aagatgcttt aacagcgcca catcttgtgc gtcgttggcc gtgacgttgg cctgaagtaa
     8461 gaccgtgtct gctaacgctt tttgcacctg cgggtcgctg aaggtgtatt tctcaaactc
     8521 tttacaggcg acgcaccagt cggcataaag atctaacatc accggtttgc ctttggcttc
     8581 aacgagcgcc tgatttaact catctaccgt tttgatttgt gtaaagttga gatgcgtctg
     8641 agtttgcgcg gtatgcgtcg caccaaatgc ccaatcctga agtgggcgca cgctaaccaa
     8701 tgccgctgca agcaggatta tttgcaccac gcgcatccag ccgcgtttgg cctttaggct
     8761 ggtgataaag gcccagccaa agaatgcgac acccagcgcc gaccacaagc gtaatcccca
     8821 tatatcacca atcactcgct ccagcaggaa gaccggcagt gcgaggatca caaaaccaaa
     8881 cgcggttttg acttgttcca tccacgggcc gcttttcggc agcaagcggt taccaaagac
     8941 ggtaattagc atcagcggca ggcccatgcc caacgcatag agataaagcg tgccgccgcc
     9001 cagccacatg ttcccgcttt gggcgatata cagcagaatc gcgctaagcg gtgcggtggt
     9061 gcatggtgaa cagatcagtc cggcaatcgc ccccataata aacactccgc cgggtgagcc
     9121 gccctgttgg cgattgctca tcagcgtgag gcgcgtttgc agcgaggagg ggagttgtag
     9181 agtaaacaag ccaaacattg acatcgccag caaggtaaag acgatggcga ggccaatgag
     9241 cacgtatggg tgctgtagcg ccgcctggaa ctgtaaccct gcggcggcaa ctaccagacc
     9301 cagcgccgtg taagtcagtg ccatcccctg cacataaata aaggtcagca acaatgctct
     9361 ggcagtggaa agccgctgtt taccgcccag cacgatgcca gaaatcagtg ggtacattgg
     9421 cagcacgcat ggcgtaaagg cgataccaat accgatcaac aacgcccaga gcgcggaaaa
     9481 gggcaattgc gcggtgggct gctcttgctg cggaacagac acaggctgtg gcgctgcgtt
     9541 gttggcgacc acttcgctta acggaacggt tttggtttct ggtggataac agaaaccggc
     9601 atcagcacag ccctggtagg tgacagttaa cgtcgctccc gcactcgcct ggttgatggt
     9661 tacgggaagt gtcagccgat cgcggtaaat ctcgcttttg ccgtaaaact catcttcatg
     9721 ccagacgcct tgcggcagct gcacgtcggc aattttcgcg tgttccggcg taatgcggat
     9781 ctgtttacgg tagaggtagt aaccgtcttt gatctgccag gtcagattaa ggtcatgttg
     9841 gttttgctga aaatcaaaag caaaggcttg atccgcgggg acaaattgtg aacgtcccgg
     9901 cgcgtcgaat aatccggcaa aaacggaagt gctgcaaagt agcaggatca gcgtaaagat
     9961 gcgttgagcc atgagaggta atctgtgtct ccgtgtgtaa caggtaaaac cagaagttcc
    10021 ggggtttgat atggatgatg agacttcagg cattccagca gtgcctgctg gtgagatacg
    10081 gtagttttta aaatcatctg cacttcgtat tcttgctcca gcttaccttc ccagtaatag
    10141 agagaggtag cgccggggat caaggtcgcg caggccgcca gtttttccgc cagcactttg
    10201 gcggctaaat cctgggctgt cgcttcatcc ggtgcggtac atagcaccac gacagacgcg
    10261 gtattcgaac ttttttcatc aagcataaac acctcgcaag aacagatgga accgcaagag
    10321 agaggtcact atacaacgga cgggggaagg atgttagtcg gcgggaaaga aagcggggcg
    10381 tgaacgcccc gcgattggtc attacagcat gaagctaccc agcacgaagc cgaagcaaac
    10441 ggccagggca acacccagag tacccgggat gaagaacgga tggttgaaga cgaatttacc
    10501 gatacgggta gtacccgtgt catccatctg taccgcagca accagcgtcg ggtaggtcgg
    10561 cagaatgaac agaccagaca ccgcagcgaa agaagcaaca gcggtcagcg gagaaacgtt
    10621 cagtgccaga gccatcggca tcagtgcttt tgcggttgca gcctgagagt acagcagagc
    10681 agaagcaaag aagaagatga cggccagcag ccacggatga ccctgaatca cttcaccagc
    10741 ggtatctttg atccagtcga tgttgttgga aacgaaagta tcgcccagcc acgcaacacc
    10801 caggatacaa atacaggcgc tcatgcctgc tttgaaggtg ctggagttga ggatgttgtc
    10861 ggtatcgact ttacagataa cggtggtcag agttgcaacg ctgagcatga tgatcaggat
    10921 tgcgttggtg gtgttcatca gcggtttttc aaccagaccc atgcttgggc tgttgatgat
    10981 tgcatagatc accacgccaa ctacgcccag caggaacagc cagacggacg tttttgcacc
    11041 ggatttgatt tcaatctgct tttcaccgcg cagttcaacc aggccctctt ccagacgctt
    11101 gcgataaatc ggatcgtcag agagtttgga gttgaacagc atagtgacca ggaaggacat
    11161 caccagaacc gccagcaggg tggacgggat gaccacggag agcagatgga ggtagctgat
    11221 gccatgacct tccatcacgg aagacatgta aaccactgcc gctgagattg gcgatgcggt
    11281 gatcgcaatc tgcgcggata ccactgcagt agacagcgga cggcaaggtt taacgccttg
    11341 ttccttcgca acttcagcga taactggcag tgtcgccaga gagatgttgc cagtaccagc
    11401 aaaaatagtc aggaaatagg tcacgatcgg tgcgaggatc gtgatgtatt tcgggttacg
    11461 gcgcagcagc ttttctgtct gatgaaccag atagtccaga ccgccagcaa cctgcatggc
    11521 agaaatagcg gcgataaccg ccatgataat ggaaatgaca tcgaacggga tgttaccggg
    11581 tttaacgcca atagcggcaa gaaccagcac ccccaatccg cctgcaaaac caatacctat
    11641 tccccccaat ctggcgccca agaagatcgc cagcaaaact atgatgagtt ctacaactag
    11701 catattagcc ttccttgttt tttaacaagt tgatattagg ttgttatttt taagttactg
    11761 ctcacaagaa aaaaggcacg tcatctgacg tgccttcttt atttgtacta ccctgtacga
    11821 ttactgttcg ctttcatcag tatagcgttt tgctttgtaa gccgggtgca tcagattctg
    11881 tacggagaaa atatcgtcaa gttccgcttc agtcaacaga ccgcgttcca gaacgacttc
    11941 acgtacactc ttaccggttt cggcacagat tttacccacg atgtcaccgt tgtggtgacc
    12001 gatgaacggg ttcaggtaag taacgatacc gatagagttg taaacgtaac cttcgcacac
    12061 ttctttgtta gcagtgatgc cgttaatgca tttttccagc aggttgtagc aagcgttggt
    12121 cagaatgtga acggattcga acatggcctg gccaatgacc ggctccataa cgttcaactg
    12181 cagctgacct gcttctgctg ccatggtaac agtggtgtcg ttaccgatga ctttgaagca
    12241 tacctggtta accacttccg gaacaaccgg gtttacttta gctggcatga tggaagagcc
    12301 cgcctgcagt tccggcaggt tgatctcgtt caggccggca cgtggaccag aagagagcaa
    12361 gcgcaggtcg ttacagattt tggacatctt cacagccagg cgtttcagcg cgccgtgaac
    12421 cataacataa gcgccgcagt cagaggtcgc ttcgatcagg tcttcagccg gtacgcatgg
    12481 gaagccagtg acttcagcca gttttttcac tgccagcgga gagtactctt tcggcgtgtt
    12541 cagaccagta ccgattgctg ttgcgccaag gttaacttcc agcagcagtt cagcggtacg
    12601 ttggatattt ttcacttctt ctttcagcag gatgctgaaa gcgcggaatt cctgaccgag
    12661 ggtcatcggt actgcgtcct gcagctgggt acgacccatt ttcaggatgt cctggaattc
    12721 gacagcttta cgttcaaagc cttcacgcag ttggttaatc gcatctacca gcttaatcag
    12781 ggaagagtaa actgcgatac ggaaaccggt cgggtaggcg tcgttagtgg actgacattt
    12841 gttaacatgg tcgttcgggt tcaggtactg atattcacct ttctggtgac ccatcagttc
    12901 cagaccgata ttggccagca cttcgttggt gttcatgttt acggaagtac ctgcgccgcc
    12961 ctggtagacg tctaccggga actgatccat gcattttccg ttgttcagga cttcatcaca
    13021 tgcggcaatg atggcattcg ctacactttt aggaatggtt tgcagctctt tgtttgccat
    13081 agctgcggct tttttaacca ttaccatacc gcgaacaaat tcaggaatat cactgatttt
    13141 gttgttgctg atatagaagt tttcaatcgc tctcagagtg tgaacaccat agtaggcatc
    13201 agctggaact tccctggtac ccaacagatc ttcttcgata cgaatgttgt ttgacatgtg
    13261 aaccttcttt ttcaagctgc caatgatttg ctttaaacac acagaatata tgtggtttcg
    13321 aatgtttttc gaccgacgat tatcccctgc atcgaccgaa tacccgagat catatgctgc
    13381 ttgaggattt ctaccgtaat ctggatcact ttaagtgtcg gtttttaccc cttaattatt
    13441 aatttgtgaa atagatcacc gctttgggat tactaccaaa aatagttgcg caaacatctt
    13501 gaaattttgc taatgaccac aatataagct gaacgcgatt cgcaacccat tcaggtagcc
    13561 gggttttacc ggctgctatt acaggagaaa cctttgcgct ggttaccttt tattgccatt
    13621 ttcctttatg tctatattga gatttcaatc tttattcagg ttgcccatgt attgggggta
    13681 ttgctgaccc tcgtgctggt tatattcacg tcagttatcg ggatgtcact ggtacgtaac
    13741 cagggcttta agaatttcgt gctgatgcag caaaaaatgg cggcgggtga aaacccagcg
    13801 gcggagatga ttaaaagtgt ttcgctgatc attgccggtt tgctgctttt attaccgggc
    13861 tttttcaccg acttcctcgg tcttctgctt ttattaccgc cggtgcaaaa gcatctgaca
    13921 gtgaagttga tgccgcattt gcgcttttct cgtatgccag gcggcggttt tagcgccggg
    13981 accggtggcg gtaatacttt tgatggtgag taccagcgaa aggatgatga gcgcgaccgc
    14041 cttgatcata aagacgatcg ccaggattaa tgtcgaaacg ccggattatg tggttatgcc
    14101 attttccggc gtttttcgtt ttggcagcaa cagccataac cccgccagca tgatcaacgc
    14161 atagagactt ttccagccga ccattgccag taacagaacg cataacagcc cgccaaccac
    14221 cgccagtagt cgataacgtc cttgcaataa tttacagcct gccagcatgc ataacagata
    14281 aatcataata aagatgccat tggcataaat aataagagcg tccagattga tctctaaagc
    14341 atgaatcacc aacgtgctca ccacacagca gccgagtacc gcattgaggg cattattcgg
    14401 gatatggcga gaagagaggc gtgccaggta gtggtcagga ttatgttgcg cctgcgacca
    14461 gaccaggcgg gcgaagctct gtatataaat gttgagactg gcaaagcagg ccagatagcc
    14521 aatcacgcag gcaatccata acgctcctac accgaacaac tgcactacaa tttttggaag
    14581 cgatgctgcc gccgccattt tttcaccata ggcgtcgaag tgtaagacga ctaccgtaca
    14641 gccccagtag actaatcctg ccagcagcag accaatcatc aatgcgcgag gaaaatcacg
    14701 ctctggattt ttaaattccg aggcaagatg ggcaaatgct tccagaccga caaaacacca
    14761 gaacatcact gataacgcag caaataaccc ggtaagttcg atattaccag gtgccgggaa
    14821 ggggatattc gcaggtttga tatcgcccgc ccaccagata gcgacaatca gcgcgacgat
    14881 aagtccggca ataacggttt gtagattagc actggaactg gcaccgcgag taccgatata
    14941 ccacaccagc gccagcgtac cgagttctgc caacaacagt tgccagctat gccagccaaa
    15001 cattgcctgg ccgaacccgg cggcaatttg tagcgcggca ggcaaaccca cgggaatgac
    15061 cgataaaaac aaccagccgg tgactcgctc aagtcgcgaa ccaaacgcca taccgacgaa
    15121 gtgcgcgaca ccgcctgcgc tgggatagtg gcgacccaga atcgcaaaca caatcgcaat
    15181 cgggaacact aagataatca aaacgggcca cgcccacagg ctgttattgc ccgctaccag
    15241 cgcagctaac gcaggaacgg caaacacgcc agtgcctaat aatgacgtcg atagcaggcc
    15301 aatgccctgg gccagcccca gttcttgttt gagtccactc atgggttgat gtccgattgc
    15361 gcccaaattt tgggcaactg cgtagatttt cgatggtagc acaatcagat tcgcttatga
    15421 cggcgatgaa gaaattgcga tgaaatgtga ggtgaatcag ggttttcacc cgattttgtg
    15481 ctgatcagaa ttttttttct ttttccccct tgaaggggcg aagcctcatc cccatttctc
    15541 tggtcaccag ccgggaaacc atgtaaggtc cggcgtcacc cataacagat acggactttc
    15601 tcaaaggaga gttatcaatg aatattcgtc cattgcatga tcgcgtgatc gtcaagcgta
    15661 aagaagttga aactaaatct gctggcggca tcgttctgac cggctctgca gcggctaaat
    15721 ccacccgcgg cgaagtgctg gctgtcggca atggccgtat ccttgaaaat ggcgaagtga
    15781 agccgctgga tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac ggtgtgaaat
    15841 ctgagaagat cgacaatgaa gaagtgttga tcatgtccga aagcgacatt ctggcaattg
    15901 ttgaagcgta atcctcgcac gacactgaac atacgaattt aaggaataaa gataatggca
    15961 gctaaagacg taaaattcgg taacgacgct cgtgtgaaaa tgctgcgcgg cgtaaacgta
    16021 ctggcagatg cagtgaaagt taccctcggt ccgaaaggcc gtaacgtagt tctggataaa
    16081 tctttcggtg caccgaccat caccaaagat ggtgtttccg ttgctcgtga aatcgaactg
    16141 gaagacaagt tcgaaaatat gggtgcgcag atggtgaaag aagttgcctc taaagcgaac
    16201 gacgctgcag gcgacggtac caccactgca actgtactgg ctcaggctat catcactgag
    16261 ggtctgaaag ctgttgctgc gggcatgaac ccgatggacc tgaaacgtgg tatcgacaaa
    16321 gccgttaccg ctgcagttga agaactgaaa gcgctgtccg taccgtgctc tgactctaaa
    16381 gcgattgctc aggttggtac catctccgct aactccgacg aaaccgtagg taaactgatc
    16441 gctgaagcga tggacaaagt cggtaaagaa ggcgttatca ccgttgaaga cggtaccggt
    16501 ctgcaggacg aactggacgt ggttgaaggt atgcagttcg accgtggcta cctgtctcct
    16561 tacttcatca acaagccgga aactggcgca gtagaactgg aaagcccgtt catcctgctg
    16621 gctgacaaga aaatctctaa catccgcgaa atgctgccgg ttctggaagc tgttgccaaa
    16681 gcaggcaaac cgctgctgat catcgctgaa gatgttgaag gcgaagcgct ggcaactctg
    16741 gttgttaaca ccatgcgtgg catcgtgaaa gttgctgcgg ttaaagctcc gggcttcggc
    16801 gatcgtcgta aagctatgct gcaggatatc gcaaccctga ctggcggtac cgtaatctct
    16861 gaagagatcg gtatggagct ggaaaaagca accctggaag acctgggtca ggctaaacgt
    16921 gttgtgatca acaaagacac caccactatc atcgatggcg tgggtgaaga agctgcaatc
    16981 cagggccgtg ttgctcagat ccgtcagcag attgaagaag caacttctga ctacgaccgt
    17041 gaaaaactgc aggaacgcgt agcgaaactg gcaggcggcg ttgcagttat caaagtaggt
    17101 gctgctaccg aagttgaaat gaaagagaaa aaagcacgcg ttgaagacgc cctgcacgcg
    17161 acccgtgctg cggtagaaga aggcgtggtt gctggtggtg gtgttgcgct gatccgcgta
    17221 gcgtctaaac tggctgacct gcgtggtcag aacgaagacc agaacgtggg tatcaaagtt
    17281 gcactgcgtg caatggaagc tccgctgcgt cagatcgtcc tgaactgcgg cgaagaaccg
    17341 tctgttgttg ctaacaccgt taaaggcggc gacggcaact acggttacaa cgcagcaacc
    17401 gaagaatacg gcaacatgat cgacatgggt atcctggacc caaccaaagt aacccgttct
    17461 gctctgcagt acgcggcttc tgtggctggc ctgatgatca ccaccgagtg catggttacc
    17521 gacctgccga aaaatgatgc agctgactta ggcgctgctg gcggtatggg cggcatgggt
    17581 ggcatgggcg gcatgatgta attgccctgc acctcgcaga aataaacaaa cccccgggca
    17641 gaaatgtctg ggggtttttc ttttggtcat ctttctagta taagattcag acacggacga
    17701 cgcgagtggc gtccagctca ttgattatgg ggaataacat gcacgtaaaa tacttagcag
    17761 ggattgtcgg tgccgcgcta ctgatggcgg gttgtagctc cagcaacgaa ttgagtgctg
    17821 ccggtcagag tgtacgcatt gtggacgagc agccaggcgc agagtgccag ctgattggta
    17881 ctgcgacagg taagcaaagc aactggcttt ccgggcaaca cggagaagag ggcggttcta
    17941 tgcgcggcgc agcaaacgat ctgcgcaacc aggcggctgc aatgggcggt aacgtgattt
    18001 atggcatcag cagcccgtcg cagggaatgt tgtccagttt tgtcccgacg gatagccaga
    18061 ttatcggtca ggtttataag tgcccgaact gatgtgatcc agaggctggg tgacaaacgt
    18121 atcattgcct gatgcgcttc gcttatcagg cctacagcag gcattgcaac tgattgaatg
    18181 cttgagaatt tgtaggccgg ataaggcgtt tacgccgcat ccggcatgac caacgatcac
    18241 gttatcagtc atttcaggct ggatggctcc agcctcgcat tagcaaagat gagattattt
    18301 cgcctgtggt gcagttttgc tggtggattg aacacgtaag tgatgcagcg cgcgtaagca
    18361 ttgatcctgg gttagcggtt gggcgttatt cgccgctacg gtgcgaacat agacctgaca
    18421 tggcacaccc gcgagttttt tcagtcgttg gctgaaatca agcattcggc gagtgatcgc
    18481 ctctatttct ttacccggcg ttgcttcacg cgttttcacc actgcgccgc taaattgttc
    18541 tttgccactt tcatctttaa agcggtaaag caccgccaga taatggtcga acaggttatg
    18601 tttccattcc ggttgggaat aggtgtcata ttccagattg ccattttcct ggcagtcaac
    18661 gtcatataac ggcaggaaat ctaacagtga ggtaatacgt aatgccagtt cacgcatttc
    18721 cgcattatta attgcatgaa tgattgctcg cgccagcatt tcaatctgca actcattgac
    18781 gactaactca catttgctac cgtcatgtag cgttaatacg aaggtaagat tttcgccttg
    18841 attatcggta agttccagcg tgttaacccg acgattaata tcggcatttt taagctcatc
    18901 aaccagaatt tctgggatgt tttctgccat tttcttaatg actttatcgc gagcttgctc
    18961 atactgaagg cgtgcagcag catccagttt atgcttttga tgcatacgac tttccagcgc
    19021 aatcagcagg tcgcgcagtt ccatgacaga cataaagaac aacgattctt tattgcgagg
    19081 ttctttaatt ttcagcgcca gtgcaatgaa attattactc ttgcgaataa caccggtatt
    19141 aacacccttg atgcttatgg ccatgtaggt tctccctaac catttctcaa ttaaataatt
    19201 aattttaatt tataagccag ataaatgggc ttggtagtaa tagttgttaa aataacataa
    19261 atagccgtac tcactctata taaacagaaa agaaaggtta attattggtg ttggctatat
    19321 aaagagtaac aacagcaatg tatatgaata attaaaggtt atgcaacggg caaagattaa
    19381 atttcccagt ttaatgacag gttgctgtac ttgcgtttgt tgctcttgcc ggatgcggcg
    19441 taaacgcctt atccggccta cagttcattg caaatccatt caatttcaat gcacaatgta
    19501 ggcctgataa gcgtagcgca tcaggctaat gtttaacttc cctgtttaat cagttactgc
    19561 tggcgtagct ggagatccag cggcgttttg ctgggttcgc cgccgatttc tcgcgccagt
    19621 ttcggcacca gataacccga caccagtgtc agcaactcac gcataatctg ccgtgcttcg
    19681 tcatcactca ccataaaatg cgccgcgccc tgtactttat cgagcacatg caggtaatac
    19741 ggcattacgc cggcatcgaa caacgcatta ctcaggtttg ccagcgtttg tgcgttatcg
    19801 ttcacaccac gtaacagaac gctctggtta agcagggtga caccgacacg gcgcaattta
    19861 gccatcgcct gacggaacgt ttcatctacc tcattggcat gattgatgtg attcaccagc
    19921 aagatttgca gcgtagaacg ggcaaagcgt tcaaccagcg cctcggtgat acgtgccggg
    19981 atcacaatcg gcagacggct gtgaatccgc agacgtttga tatgcgggat ggcttccagt
    20041 tgtgtgagca accagtccag ctcgtgatct ttcgccatca gcggatcgcc gccggagaaa
    20101 atcatctcgt ccagttccgg atgcgcagca acatactcaa gagcagtctg ccagttacgc
    20161 ttgttgccct gattttcggc ataggggaag tggcgacgga agcaatagcg gcaatttacc
    20221 gcgcagccgc ctttgaccag caaaagcgcc cggttgtggt atttatgcaa caaaccaggc
    20281 actacgctgt gctgttcttc cagcgggtcg gtggagaatc cggacacgac gacaaactca
    20341 tcttgcgagg taagtacctg acgcaaaaga ggatcgtccg gattgccttt ctccatgcga
    20401 tcgataaatg agcggggcac acgcagggca aacagctttt tggcgctgcg tccggccaac
    20461 agtttttcgt ccgcgtctat attcaaaaga cgcagaagtt catcaggatc ggtcacaaca
    20521 tcggcaagtt gcgttaacca atcttctctg gatggggtat ttagggttac aatatgcgcc
    20581 attttgtggc ttagctacca attaacaaat ttcagagggc cttatggcaa cgtactatag
    20641 caacgatttt cgtgctggtc ttaaaatcat gttagacggc gaaccttacg cggttgaagc
    20701 gagtgaattc gtaaaaccgg gtaaaggcca ggcatttgct cgcgttaaac tgcgtcgtct
    20761 gctgaccggt actcgcgtag aaaaaacctt caaatctact gattccgctg aaggcgctga
    20821 tgttgtcgat atgaacctga cttacctgta caacgacggt gagttctggc acttcatgaa
    20881 caacgaaact ttcgagcagc tgtctgctga tgcaaaagca attggcgaca acgctaaatg
    20941 gctgctggat caggcagagt gtatcgtaac tctgtggaat ggtcagccga tctccgttac
    21001 tccgccgaac ttcgttgaac tggaaatcgt tgataccgat ccgggcctga aaggtgatac
    21061 cgcaggtact ggcggcaaac cggctaccct gtctactggc gctgtggtta aggttccgct
    21121 gttcgtacaa atcggcgaag tcatcaaagt ggatacccgc tctggtgaat acgtctctcg
    21181 cgtgaagtaa tgcggttgtg gtgcggcctg caggctgcac catcacttat tcaggtcaga
    21241 gatgatgaaa cgccttatcg ttcttgtttt gcttgccagc acgctgctca cgggctgtaa
    21301 caccgctcgc ggtttcggcg aagacatcaa acatctcggc aactccatct ctcgcgctgc
    21361 cagctaattt ttcttctctt ccgaaaaatc atcagatttc cgtcattttt ggcgatgttg
    21421 tctattatta atttgctata ggcaaacata aataacatta cctaaaagga agacgttatg
    21481 gtgaagaaga caattgcagc gatcttttct gttctggtgc tttcaacagt attaactgcc
    21541 tgcaacacca cgcgtggcgt tggtgaagac atttctgatg gcggtaacgc gatttctggc
    21601 gcagcaacga aagcgcagca atagcaataa gcaataacgg tacgacaggt atgtcgtgcc
    21661 gtttgttttt tctgcgatag tcactaaggt aatagttgaa attcccctgc cacctggcaa
    21721 aatatccgtt caaccatcag ctttgcagga cgacctgcaa acgcctcttt tcaccgggga
    21781 cggccccaat tctccggagc ctgatatgtc ctggattatc ttagttattg ctggtctgct
    21841 ggaagtggta tgggccgttg gcctgaaata tacccacggc tttagtcgtt tgacgccgag
    21901 tgttattacc gtgacggcga tgattgtcag tatggcgcta cttgcctggg cgatgaaatc
    21961 gttaccagta gggacggctt atgccgtgtg gacgggtatt ggcgcagtcg gcgcggctat
    22021 cactggcatt gtgctgctcg gtgagtccgc taacccgatg cgcctggcga gtctggcgtt
    22081 aatcgtattg gggattattg gtctgaaact cagcactcac taactaccgg gctgctgtac
    22141 ccaaataaac ttactgacat caaacccttc ccgggtcgcg actgccagca tctcctgttt
    22201 cacttcgtca gaaatggttg gcgtgcggga gagtatccac aggtagtcgc ggtccgggcc
    22261 gcaaaccagc gcatggcggt attcccgatc gagtgcaata acgttataac cgccatagaa
    22321 aggaccaaag aatgacactt tcagcgcagc gcgagttggt gcgccggtaa agtacgcttt
    22381 cccttcactc tgctgccaca ttcctctgtc agggttatag cctttattaa tgacattcag
    22441 gccgccgtca tcacgcaggc tgtatgttgc ggtgactttt tccagtccac gttcaaaacg
    22501 gtgatcaaaa cgggcaatct cataccaggt accgagataa cgtttggcgt cgaaattatt
    22561 cactactgtt acgccacgcg gcggcgtagg agaactgcag gcaacgacca gaaatgcagc
    22621 tgtcgctgcg gcaacgagag ggagcaggcg cataaatgtt tccttactgg tttttttcta
    22681 agtgtagatg acagcaagga aaagcgcaga aaaggtccga aaattcggac ccgatgaaat
    22741 tttactgtag agcgttgaga atctgccagg cggcggcgac tctcgctgga ttgggatagt
    22801 ttttgttagc cagcatcact atacccagct ctttttctgg aataaacgcg acatagctac
    22861 caaatccgcc ggttgcccct gttttatgta cccatgatgc gcgtactgca ggagttgggg
    22921 gcgtaatcgc ttttacgggg tgtgctgcca gtgcaatttt attgccactg ccgttaatga
    22981 tgctgtcagg atttaccggc cagtccagca tttcccagcc caggccctga tacatatcgc
    23041 cggtttgcca gtagcgagat tgtgccagtt gtatcccttg ctgaagtgtt ttgtcgttga
    23101 tatcacgggg attcatattg cttcgtaccc agcaggccat atcttcaatg gtcgacttca
    23161 caccataagt ttcagcatct aacgcccctg gcgaaacatg aactgccttg ccttcgcgat
    23221 atccccaggc gtaattcttt tcttctgcgg gcggtacatt aatccacgta tggttgagtt
    23281 tgagtggctg gaagacacgt gtttgcatcg cctgctcaaa actcaaacca gacggcttca
    23341 cagccagtgc gccgaacaaa ccgatactgg agttggcata cagacgttgt gttcctggcg
    23401 cccatgcagg ctgccagttt tgatagaagc gcagcaagtc gcttgaggat ttcacctcat
    23461 ccggcacctg caatggcagg ccgccagcag tgtaggttgc gagatgtaat agtgtgatcc
    23521 cattccactg tttagcggta agttcaggcc agtattttgt tgtgggatcg cttaacttga
    23581 tttcccctcg agcaatagcg tcgccaccaa gcacgcccgt aaatgttttg ctgaccgaac
    23641 ctaactcaaa caacgtttgc tgtgtgacgg gctgcttttt ggcgatgtcc gcatagcccc
    23701 aggtaaagta ataaggttta ccctgataaa ttaccgccac cgccataccg gggatctttt
    23761 gttgctctat aagcggggta attgtgcgat gcacaatatc gttgatttgt tgaggggcag
    23821 caaatgtgga gcaagaggcg gtaattaata aggcgcagag cgtcgttttg aacataaggt
    23881 ctggtttcca tacaaaacgg cccgccatag gcgggccgga tttacattgg cgatacgtta
    23941 gattgtaacg ataccaatca gcgtgacaac tgtcaggata gcagccagac cgtagaaaac
    24001 ccatttgccc gcaggcacgt gaattttcag atcgtgcatc gcgtggtgca tacggtgtaa
    24061 accacaccac agcggcagaa cgatcatcag gaacaggaat acgcgaccaa tgaagctctg
    24121 cgcgaacgcc agaacgcgct cgtagctcag cgcatcaccc ggaaacagcc ccagtggcag
    24181 cagaataccc accagcagga tcatcaccgg cgcaataatt gcgctccaca taccaccggc
    24241 cccgaagagg ccccagaata ccggttcgtc agaacgcttt ggatttggat taatcatctc
    24301 aggctcctta ccagtacagg gcaacaaaca ggattacgat ggtggcaacc acagttaccg
    24361 cccagagact tttgataatt ggctctggtc ccattttttc gtcttttaca atgatattgg
    24421 ccgctttcgg tgccagttca aaccaggttt tggtgtgcag cagagctgcc gccagagtga
    24481 tcaggttaat gatcacgata accgggtttt gtaaaaagtc gacgaatccc gcccaggctt
    24541 ccgggccatt tttcagggca aacagcccga aaatcagttc aatgctgaac cacacagccg
    24601 gaaccgccgt gccttcgcgc agcatgtaaa agcgataaaa cggcaatttt ttccaccagg
    24661 tggacgtcat tggccgtaca tacggtttac gtttagtcgt catgttgcac tccttagcgt
    24721 ggtttcaggg tcgcgataag aaagtctttc gaactttcta ctttgccctg ctgaatggcc
    24781 gcagccggat cgacgtgttt cgggcagact tcggagcaat agcccacgaa agtacagctc
    24841 catacgccgt tctggctgtt caactgcgcc atacgctcct tcttaccgtg gtcgcggcta
    24901 tcttcgttat aacgatgcgc cagcgtaatg gcagccggac cgatgaactc tgggttcagg
    24961 ccaaactgcg ggcacgcggc gtagcacaga ccacagttga tgcaaccgga gaactggtga
    25021 tactttgcca tctgcgccgg ggtctggatg ttagtaccct gatccgcggt gcgggagttg
    25081 ccgatgatgt acggtttgat cgcttccaga ctttcgatga agtgggtcat atcgaccacc
    25141 agatcgcgtt caatcgggaa gttagctaac gcttcaacct tcataccgtc ggtgtaatca
    25201 cgcaggaagg ttttacatgc cagttttggc acgttgttaa ccatcatgcc gcaggaacca
    25261 caaatcgcca tacggcagga ccagcggtaa ctcaggtccg gtgccaggtt gtctttgatg
    25321 tagcccagcg catccagtaa tgaggtagtt gcgtcataag gcacttcata gaatgcgcta
    25381 tgcggtgcgg tatcgacttc cgggttatag cgcaccacct caattttcag gtttttcatc
    25441 tcagccattc gccttctcct tcttattggc tgcttccgcc ttatcggctg catccgcttc
    25501 gccaccgtaa acgcgtttag ctggcggcag cgtagtaatc ttcacgtcgc tgtactccag
    25561 gcgagtcgtg ccatcagcat cgcggaaggc gagggtgtgt ttgaggaagt tgacgtcgtc
    25621 acgctcggtg caaccttcgt ccagacgctg gtgtgcgccg cgggactctt tacgtgccat
    25681 tgcggagtgc gccatacatt cagcaacgtt cagaccgtgg cccagttcaa tggtgtagag
    25741 caggtcggtg ttgaacacgc tggaggtgtc ggtgatgcgc acgcgcttga agcgttcctg
    25801 cagctctgcc agcttgtcga tggttttctg catcagttcc ggcgtacggt agataccgca
    25861 accttcttcc attgccaggc ccatttcgtc gcggatcttc gcccagtttt cgccgccatc
    25921 ctggttaacc agatctttca gacgttgttc aacgccagct gcctgcgctt caattgccgc
    25981 ttcgttgcca ttaccggcag ttgctgcacg ctctgtcgct tgttcaccgg ccagacggcc
    26041 gaagaccacc agttccgcca gggagttaga acccagacgg tttgcaccgt gcagaccaac
    26101 agaggaacat tcacccacgg cgaacagacc tttaatgcgg gtttcacagt tctgatcggt
    26161 ttcgataccg cccatggtgt agtgtgcggt cggacgtacc ggaatcggtt ctttaaccgg
    26221 atcgacgcca acgtacgctt tcgccagttc gcagatgaac ggcagacgtt catgcagttt
    26281 tttctcgccg aggtgacgca ggtcgagata aaccacatcg ccacgcggcg tggagatggt
    26341 gttgccttta cgccattcgt gccagaaggc ctgagaaact ttgtcgcgtg gacccagttc
    26401 catatatttg tttttcggct cgcccagcgg agtttccggg cccatgccgt aatcttgcag
    26461 ataacggtag ccatttttgt tgaccagaat accgccttca ccgcggcagc cttcggtcat
    26521 caggataccg gaacctggca gaccggttgg gtgatactga acgaattcca tgtcacgcag
    26581 cggaacgccg tggcttagcg ccatacccat accgtcaccg gtaacgatgc cgccgttggt
    26641 gttgtaacga taaacgcgac ccgcaccgcc agtagccata acgaccgcgt tagcacggat
    26701 ctgcaccagc gtgccttcca tcatgttcat tgctaccagg ccgcgaacat gaccatcatc
    26761 aaccagaata tccagcacga aatgttcgtc aaaacgctgg atctgcggga attgcagaga
    26821 ggtctggaac agcgtgtgca gcatatggaa gccggtctta tcggcggcga accaggtgcg
    26881 ctcgattttc atgccgccga agcgacgtac gttgacgcta ccatccgggc gacggctcca
    26941 tgggcacccc cacagttcca gttgggtcat ttcggttggg cagtggtgga cgaaataatc
    27001 cacgacatcc tgctcacaca accagtcgcc acccgctact gtatcgtgaa agtgatattc
    27061 gaagctgtca tgatcctgcg cgacagcggc ggagccccct tctgcagcaa cggtatggct
    27121 acgcatcggg tatacttttg agattagtgc gatttttgca tttggatttg cctgcgcggc
    27181 agcaattgca gcacgtaatc ccgcgccacc ggcgcctaca atggcaagat cggcttgaaa
    27241 ggtttgcacg acattcctcc agattgtttt tatcccacag ccccgtactt cagggtaagt
    27301 gcctgaaagt tacggtctgc gaacgctatt ccactgctcc tttataggta caacagtata
    27361 gtctgatgga taagtctgaa atttgacgag atcgattttt ttagtgcgca agagggctaa
    27421 attatcactg aagatgatta atttaattac taaaccatca gatcgtgctt tttttagtca
    27481 ctgcccaccg ctgtttgatt cctgcgtaaa atttgtctcg ccgtcgcgtt gcgagtagac
    27541 ttcgtgccct tgtcaaaaac tggagattta actatgagcg aaacggcatc ctggcagccg
    27601 agcgcatcca ttcctaactt attaaaacgc gcggcgatta tggcggagat ccgtcgtttc
    27661 tttgccgatc gtggagtgct ggaggtggag acgccttgta tgagccaggc gacggtaacc
    27721 gatattcatt tggtcccgtt tgagacacgt ttcgttggcc ccgggcattc gcaggggatg
    27781 aatctctggt taatgaccag cccggaatac catatgaaac gcctgctggt tgccggttgt
    27841 gggccggtat tccagctgtg ccgcagcttc cgtaatgaag agatggggcg ttatcacaac
    27901 cctgagttca ctatgctgga gtggtatcga ccgcactatg atatgtaccg gttgatgaac
    27961 gaggtggacg atctcttaca acaggtgctg gactgcccgg cagcagaaag cctttcttat
    28021 caacaagctt tcttgcgtta tctggaaatt gacccgctct ctgccgacaa aacgcaactg
    28081 cgggaagtcg cagcgaaact ggatttgagc aatgttgctg ataccgaaga agaccgcgac
    28141 acgctgctac aattgctgtt tacctttggc gtagagccaa atattggcaa agaaaaaccg
    28201 acctttgtgt accactttcc agccagccag gcatcactgg cgcaaatcag taccgaagat
    28261 catcgggtcg ctgaacgctt tgaggtttat tataaaggta ttgagctggc gaatggtttc
    28321 catgaattga cggatgcccg tgagcagcaa caacgctttg aacaagataa ccgtaagcgc
    28381 gcggcgcgcg gtttgccgca gcaccccatt gaccagaatc tgattgaagc cttgaaagtc
    28441 ggtatgcctg actgttccgg cgtggcatta ggtgttgatc gtctggtgat gttggcgctg
    28501 ggcgcggaga cactggctga agtcatcgcc tttagcgttg accgggcata attctgaaaa
    28561 attactgaat taacagcgaa gaatggcgtg ataagcgcca ttctgtagca tatgttttca
    28621 tttttccgtt aaaggccaca atttaagact ggatatttga tatcatccag gtatcaattc
    28681 tgtattggtt tttacgctgc ctgctcaatg ttgcgcagtg cagttcagtg ggcacgtgtt
    28741 atacacgcgc tgaaatgaag gatggtttca tgcctcacac gataaaaaag atgagtctga
    28801 taggactcat attgatgatc tttacttccg tatttggatt tgccaatagc ccatcggctt
    28861 attacttaat gggttatagt gcgattccct tttatatatt ttctgcattg ttattcttta
    28921 ttccattcgc cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggcggta
    28981 tctattcctg gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt
    29041 ggttttcctc ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct
    29101 caacattcct ctatggtagc gacatgaccc agcactggcg tattgccgga ctggagccta
    29161 cgcaggtggt tggtctgctg gcagtagcat ggatgattct ggtcaccgtc gttgcttcaa
    29221 aggggattaa taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga
    29281 atttagtatt gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc
    29341 aggatattaa tttccttgca tcaccgaacc caggttatca gtccggtctg gcaatgctat
    29401 cgtttgtggt atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg
    29461 ataaaacgga aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta
    29521 tttcaatcgg ttattcgctg gcaatatttt tatggggtgt cagcacaaac tggcagcagg
    29581 tattaagtaa tggttccgtt aacctcggca atattaccta tgtgctgatg aagagcctcg
    29641 gggtgacgct gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggtgtat
    29701 ggtttgcgcg tattaccgga ctttcgatgt tcctcgctta taccggtgcg ttctttacgc
    29761 tttgctattc accgctgaaa gccatcatcc aggggacgcc gaaagcgttg tggccggaac
    29821 cgatgacgcg cctgaatgcg atggggatgc cttctatcgc catgtggatg cagtgcgggt
    29881 tggttactgt cttcatcctg ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta
    29941 acaagctgac gctgatggcg aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg
    30001 ctttcccgtt ctttaaagca cgtcaggatc tcgacagacc gtttgtgatt ttcaaaacgc
    30061 atttgtcggc aatgattgcg acagtggttg tcgtactggt ggtgacattt gcgaacgtct
    30121 tcaccatcat tcaacctgtg gttgaagccg gagactggga cagcacattg tggatgattg
    30181 gcggccctgt cttcttctcg ctgttagcga tggcgattta ccagaactat tgcagtcgca
    30241 tggcgaataa acctgagtta gctctcgact gatcatatga aatcctgcaa ctcatcgtct
    30301 ctccggcgat gagttgtcag gaagtaatgc gtatggatga tactctccag ggccggtgat
    30361 aactgaagat gatgttaagc tgctaaatga gccacgttga catcatttgg cagcttcatg
    30421 cattacaaac ttcccgcctg acgacctttg cccgcagaag ttaatgttct ccccgtttgc
    30481 ttaccgttga ggctttccag acgcatctgg aagggcggga atggcatatc gataccgtgg
    30541 gcatggaagc ctgccagaat tagctggtgg atctcatggc gtagcggcat acggtgaccc
    30601 atctcagcgg cgtaaatacg cagctcgaaa atctgaatcc cctgttgcag atccaccagg
    30661 aagacttccg gtgccgggtt gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg
    30721 atttccgtca cttcttcgct attggcatcg gcaggggccg gaatcgtcaa caccacgcgc
    30781 gtgaccgagt cagagagcga ccagttgata aactgctcgg taataaacgc cttgttcggc
    30841 acgattatct ctttacggtc ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc
    30901 acgctaccag taagatcgcg gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac
    30961 aggatgatca ggccagagat aaagttggcg aaaatttcct gcaaaccaaa accgagacca
    31021 acaccgagcg cggcaaccag ccactgcaat ttcgaccact caataccaat cattgagaag
    31081 ccgaccagcc cgccaatcag catcagcaga tatttggtga tggtggtgat ggcgtaaccc
    31141 gtacctggcg ttaaatccag gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg
    31201 cgcaccagct gcgtggtgat gataaacacc agaatggcaa tcagcaccgc accgagggta
    31261 attggctcca gactttctac gccctgtacc gtggaggtga catcccacag cgaaatattt
    31321 tcgaggaagc cgaaagcgga atggatttct gaccacagca caatgacaga aagcagggcg
    31381 atcaacatca aaattgagcg caccagccgc aaggattgcg cactgatggc atcgagatcg
    31441 acttcgcttt catcgacttc aattgctcct tccgggctac tgtgatgatg cgcttcctct
    31501 tcgccacgcg cacgttgcgc taacatctct gcccggcgat gcttcgcccg gtcaaacgcc
    31561 agcctgcggc gctggatcag catccagcgg cggataacgt gataaaccac cagtagcagg
    31621 aaccagatgg caaccgaggt ttcaagcctc gctaacagtg cctgcgccgt tgccagataa
    31681 cccaccgccg acgccagaat ggcgaccagt ggcgcgccaa tcatcatgtt ccacagcata
    31741 tggttggtaa tgttgtcgcc gctgccttct ttgttgagat acagcgggat cccggccttt
    31801 ttcaggctga gggtgaccac cgccagcgca ccgcaaatga ggataaagca aagccgtccc
    31861 agcgaaccgg aaaactcgcg gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagt
    31921 ggcacaataa gcccgatgct catcaggtag tagcgcatcc cacgggaaac ccgttcgcgc
    31981 ggccagccaa aatgagcgat aaacaagccg ttcgggcggg caaaagtagc gcaaatcatc
    32041 actacccaca gcagcggcac ggtggctgtt acgccatcgc caatcgcgac cgccagcgga
    32101 taaggccacg cctcgcgcaa gccgtaaccc agcgtcatcc acagcaccgg caacggtgac
    32161 gcgacgagaa tcgaccagaa aagagtgcgt aacgtcagcc agaagtgatc ctgagtcact
    32221 ttgccgactt tcgccgccga acgttcaaga aaacgggtga aatagcggcg cgagtaaata
    32281 ctgcaaccga ccagaatcaa cgcgccaaac agcggcaaaa tcgtctcttt gctggtcagc
    32341 atcatcacac tggctttgcc caactgactg aaggtgtcca gcgaaatgag acgacgcaga
    32401 tcctgggcga tttccagcgg ccaggcgatg gtcatcgggc gcacgtcaga ggtccagaac
    32461 agatagcggt gcgttgcttc gttcacctct ttcagcgcat cctccagttg cccgttggag
    32521 actttcagct tggtcagttc cagtagtagc gtgtcgccac cctgcaacaa tgagttcagc
    32581 aactcacgct gagtgcgcag ttgtgcttcc agaatacggt tttgctcggc ggtcagcggc
    32641 tgaccgtcgg cctgatggat ttgccgtagc agcggctgtt tattaagcag atcctcataa
    32701 cgtaaccgtt gcacacgcaa ctgcgccatt tcggtatcaa gctgttgtgg ttttggcatt
    32761 tctggtagcc gtgccacctg cgcccgcagc gcttcaccga gcaggttgga cgatcccagc
    32821 cattgcgact gttcacgcag cgtattcaac gcctgccgga cctgtaacgt ctggctggca
    32881 gcctgacgct gttgcgaggc aacgagatcc atccgctgcg cctgttgatt cagtgccgcc
    32941 gatagttcgc ggttaatttt gaattgcgcg acgatatctt tcggcaaatc ggcactgttt
    33001 tctgccagta attcggtact ttccagcgcc cgctccgcct caagctgacg ttggctattc
    33061 aactgattac gtaaggcctg caaatacgca tccagttgct ggctctcttt ttccgccagc
    33121 tctgagcgca agcgcgctaa ttcctgacgg ttattggcag acagctgcgc cagttccagt
    33181 tcatcaacga gcgctttcag gcgtgcggag tcagactgca acgcgaaatt ttgtgcctga
    33241 ttgagcggag tattgccggt aagcgttccc aggcggcgct cgatctcatt taactgacgg
    33301 cgggcatcgg tttgctgttg cggcagttga ttcagtgaat cggcaatctc gcgggcgcgc
    33361 tcctgctctt gctgggcctg acggctctta tccagcaact gactgctgac ctggagaatt
    33421 tcctgattca gcgcatcggt agacattccc ggcgacacgc tgcgcggctc gtcacgcatg
    33481 ttgtttaatt gtgcgcgcag agtagcggag agtttcgggt aattatcgat aacttgctga
    33541 tattgtttga tgcgctcaag ggaacctttt cgttcctcaa gcgcatttaa ggcagactgg
    33601 agtgcctcta tgacttctgg ctgcgcgggt ttcgccgctt ttgcctgctc cagttcctgg
    33661 gtgatttgtt tgctatcggg ggccgtcgcg gcgtacgccc cccaactgag gcaccaggcc
    33721 atcagaaaag tgataatcag gcgcacgtca gcgtttcctt tgatggatta gacctggtct
    33781 tttttgtcgt caaccaatgg gctggcgtcg tgttctgctt cgatctcttc agcaggaagc
    33841 ggggcaggtt cagcgtctgg cgtaacaaag gtttcggtag atactgccag cggctggcca
    33901 attttcgtga cagacaggct ttccagttgc tcaaccagat tcactttacc cggtgcaaac
    33961 aggttgataa cggtggaacc gagtttaaag cgacccattt cctggccttt aagcagtgcc
    34021 acagaaccgt cgttttcccc ggcaggccag gtccagcgct tgatgatacc ttcgcgcggc
    34081 ggcgtaatgg tgcccgccca gaccgtctca atgctgccaa caatcgtcgc tccgaccaga
    34141 atctgcgcca ttgggccaaa ttcggtatcg aaaaggcaaa tcacgcgttc gttacgggca
    34201 aacagattcg gcacgttctg agcggtgaga tggttaacgg agaagagatc gcccggcacg
    34261 tagatcatct cacgcagaat accgttgcac ggcatgtgta cgcggtggta gtcacgcggg
    34321 gagaggtaag tggtcacaaa cgcaccgttg cggaacaggt ccgccatcag atagttgcct
    34381 gccagcaagg cttcgaggct gtagttgtgg cctttggctt gcaggatttt atcttcttcg
    34441 attttaccca actggctgat aacgccatcg gcaggcatga ccaggacatt cggatcggta
    34501 tcgattgggc gtacttcgtc acgcagcgga cggacaaaga attcgttaaa ggtgcggtag
    34561 ctggcggtgt ccggcttttg cgcctctttc atgtcgacct tgtagtattt aacgaacaga
    34621 tcgataacca gttttgtcag ccatcctgcc cgcttgcttg cgccccaacc cgccaggcga
    34681 gtaagccata gtttcggcag aatgtactgt agcgaaagtt taaatgaatt taacaaggta
    34741 gcctccaggc cattgttttg tcgttcctga tccggcctac atgccggatc ctgaaaaaaa
    34801 ggggacgatt ctaacgacgg ttagcttaat tgtcagtcat ccgtatcaga aaagttttta
    34861 cgcgttttta cctgcgccat gctttccaga atacggtgat agttttcgaa acgggtttcc
    34921 gcgatttttc cttcctcaac cgcttcccgg atagcgcagc ccggatcggt atcgtgtttg
    34981 caatcgcgat atttacacag acctaaatag tcatggaatt cgacaaagcc ctgagtgatt
    35041 tgttccggct ccaggtgcca gaggccgaac tcacgcacac caggggaatc aatcacatca
    35101 ccgccgtgcg ggaagtgata cagtcgtgcg gcggtcgtgg tgtgctgacc gagacccgag
    35161 ttgtcagaga catcgttggt caggatctct ttttgcagcc ccagcaacgc gttcagcagg
    35221 ctggatttgc cgacgccaga ctggccggca aaaatgctga tgcgcccggt caacgcctct
    35281 tccagcggtt ttagcccatc ctgagtatgg ctggaaacca tcaatacgcg ataaccaatg
    35341 ttgcggtaga tatccatctg ctcgttgacg aacgccatgc cttcgtcgtc cagcaggtct
    35401 atcttattga gcacaataat cggctcaatc tgcaaggttt cgcaggccac caggtaacgg
    35461 tcgataatat tgagcgacag ctccggcaaa atggcggaga caatgacaat ctggtcgatg
    35521 ttggcggcaa taggtttcac gccgtcgtag aaatccggac gcgtcaacac tgaggtgcgc
    35581 tcatgcaccg cttccacgat ccctttgaca tttacgcctt ccgccgccgg tttacccgga
    35641 cgccagacta cgcggtcgcc ggtcaccagc gaacggatgg tacggcgaat attgcagcgg
    35701 tgaacgtcgc catcggcgga ttccacatca gcgtgcatac caaagcggct gatgacgata
    35761 ccttcatcag gctcgccaaa cagattgtcg tcgtagtcgg gcttctcctt agacgtttta
    35821 agacgacgct ggtgattggc gttcacgcgg cgctgctggc ctttggagag tttattttta
    35881 ctcaatcgta cagactcctg gtcgcccctg atgggcaaaa catctatgat acacgcaatt
    35941 gtggatcaat atagtcactg tgaatgggtg gaaaatagca tgagtgccaa tgaaaacaac
    36001 ctgatttgga tcgatcttga gatgaccggt ctggatcccg agcgcgatcg cattattgag
    36061 attgccacgc tggtgaccga tgccaacctg aatattctgg cagaagggcc gaccattgca
    36121 gtacatcagt ccgatgaaca gctggcgctg atggatgact ggaacgtgcg cacccatacc
    36181 gccagcgggc tggtagagcg cgtgaaagcg agcacgatgg gcgatcggga agctgaactg
    36241 gcaacgctcg aattcttaaa acagtgggtg cctgcgggaa aatcgccgat ttgcggtaac
    36301 agcatcggac aggaccgtcg tttcctgttt aaatacatgc cggagctgga agcctacttc
    36361 cactaccgct atctcgatgt cagcaccctg aaagagctgg cgcgccgctg gaagccggaa
    36421 attctggatg gttttaccaa gcaggggacg catcaggcga tggatgatat ccgtgaatcg
    36481 gtggcggaac tggcttacta ccgcgagcat tttatcaagc tgtaaaattt ttcctggtca
    36541 cgtaagcgcc ggaatccact tgcggcggcg tgattttgac gctaaattga acgtcttgct
    36601 gataatttca gcacttgaga taaaaacgca aaaaaaactt ttttgggggg ttgcagaggg
    36661 aaagatttct cgtataatgc gcctcccgta acgacgcaga aatgcgaaaa ttacgaaagc
    36721 aaaattaagt agtacgcggg aatagctcag ttggtagagc acgaccttgc caaggtcggg
//
LOCUS       DAZGXZ010000038        36678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000038 DAZGXZ010000000
VERSION     DAZGXZ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.038"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            378..1595
                     /gene="alaA"
                     /locus_tag="ACHKJF_001985"
     CDS             378..1595
                     /gene="alaA"
                     /locus_tag="ACHKJF_001985"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP0949345.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1679..2278
                     /gene="yfbR"
                     /locus_tag="ACHKJF_001986"
     CDS             1679..2278
                     /gene="yfbR"
                     /locus_tag="ACHKJF_001986"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0949346.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2337..4169)
                     /locus_tag="ACHKJF_001987"
     CDS             complement(2337..4169)
                     /locus_tag="ACHKJF_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0949347.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4256..4906)
                     /gene="hxpA"
                     /locus_tag="ACHKJF_001988"
     CDS             complement(4256..4906)
                     /gene="hxpA"
                     /locus_tag="ACHKJF_001988"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP0949348.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLSHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4917..5411)
                     /locus_tag="ACHKJF_001989"
     CDS             complement(4917..5411)
                     /locus_tag="ACHKJF_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP0949349.1"
                     /translation="MEMTNAQRLILSNQYKMMIMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5494..5949)
                     /gene="yfbV"
                     /locus_tag="ACHKJF_001990"
     CDS             complement(5494..5949)
                     /gene="yfbV"
                     /locus_tag="ACHKJF_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP0949350.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6287..7489
                     /gene="ackA"
                     /locus_tag="ACHKJF_001991"
     CDS             6287..7489
                     /gene="ackA"
                     /locus_tag="ACHKJF_001991"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP0949351.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7564..9708
                     /gene="pta"
                     /locus_tag="ACHKJF_001992"
     CDS             7564..9708
                     /gene="pta"
                     /locus_tag="ACHKJF_001992"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0949352.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVARLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9898..11418
                     /gene="yfcC"
                     /locus_tag="ACHKJF_001993"
     CDS             9898..11418
                     /gene="yfcC"
                     /locus_tag="ACHKJF_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP0949353.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11451..11993)
                     /gene="yfcD"
                     /locus_tag="ACHKJF_001994"
     CDS             complement(11451..11993)
                     /gene="yfcD"
                     /locus_tag="ACHKJF_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP0949354.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12051..12602)
                     /gene="yfcE"
                     /locus_tag="ACHKJF_001995"
     CDS             complement(12051..12602)
                     /gene="yfcE"
                     /locus_tag="ACHKJF_001995"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP0949355.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAELLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12658..13302)
                     /gene="yfcF"
                     /locus_tag="ACHKJF_001996"
     CDS             complement(12658..13302)
                     /gene="yfcF"
                     /locus_tag="ACHKJF_001996"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP0949356.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13438..14085
                     /gene="yfcG"
                     /locus_tag="ACHKJF_001997"
     CDS             13438..14085
                     /gene="yfcG"
                     /locus_tag="ACHKJF_001997"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP0949357.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14142..14504
                     /gene="folX"
                     /locus_tag="ACHKJF_001998"
     CDS             14142..14504
                     /gene="folX"
                     /locus_tag="ACHKJF_001998"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP0949358.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKDEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14525..15418
                     /locus_tag="ACHKJF_001999"
     CDS             14525..15418
                     /locus_tag="ACHKJF_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP0949359.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15466..16368)
                     /gene="rpnB"
                     /locus_tag="ACHKJF_002000"
     CDS             complement(15466..16368)
                     /gene="rpnB"
                     /locus_tag="ACHKJF_002000"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP0949360.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16565..17338)
                     /gene="hisP"
                     /locus_tag="ACHKJF_002001"
     CDS             complement(16565..17338)
                     /gene="hisP"
                     /locus_tag="ACHKJF_002001"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP0949361.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVSGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17346..18062)
                     /locus_tag="ACHKJF_002002"
     CDS             complement(17346..18062)
                     /locus_tag="ACHKJF_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0949362.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18059..18745)
                     /gene="hisQ"
                     /locus_tag="ACHKJF_002003"
     CDS             complement(18059..18745)
                     /gene="hisQ"
                     /locus_tag="ACHKJF_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP0949363.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18835..19617)
                     /gene="hisJ"
                     /locus_tag="ACHKJF_002004"
     CDS             complement(18835..19617)
                     /gene="hisJ"
                     /locus_tag="ACHKJF_002004"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP0949364.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19839..20621)
                     /gene="argT"
                     /locus_tag="ACHKJF_002005"
     CDS             complement(19839..20621)
                     /gene="argT"
                     /locus_tag="ACHKJF_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP0949365.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20887..21456)
                     /gene="ubiX"
                     /locus_tag="ACHKJF_002006"
     CDS             complement(20887..21456)
                     /gene="ubiX"
                     /locus_tag="ACHKJF_002006"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP0949366.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21551..23068)
                     /gene="purF"
                     /locus_tag="ACHKJF_002007"
     CDS             complement(21551..23068)
                     /gene="purF"
                     /locus_tag="ACHKJF_002007"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP0949367.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23105..23593)
                     /gene="cvpA"
                     /locus_tag="ACHKJF_002008"
     CDS             complement(23105..23593)
                     /gene="cvpA"
                     /locus_tag="ACHKJF_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP0949368.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23852..24514)
                     /gene="dedD"
                     /locus_tag="ACHKJF_002009"
     CDS             complement(23852..24514)
                     /gene="dedD"
                     /locus_tag="ACHKJF_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP0949369.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24504..25772)
                     /gene="folC"
                     /locus_tag="ACHKJF_002010"
     CDS             complement(24504..25772)
                     /gene="folC"
                     /locus_tag="ACHKJF_002010"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP0949370.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVISIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25842..26756)
                     /gene="accD"
                     /locus_tag="ACHKJF_002011"
     CDS             complement(25842..26756)
                     /gene="accD"
                     /locus_tag="ACHKJF_002011"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP0949371.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26912..27571)
                     /locus_tag="ACHKJF_002012"
     CDS             complement(26912..27571)
                     /locus_tag="ACHKJF_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0949372.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27654..28466)
                     /gene="truA"
                     /locus_tag="ACHKJF_002013"
     CDS             complement(27654..28466)
                     /gene="truA"
                     /locus_tag="ACHKJF_002013"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP0949373.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28466..29479)
                     /locus_tag="ACHKJF_002014"
     CDS             complement(28466..29479)
                     /locus_tag="ACHKJF_002014"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0949374.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEACDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29545..30681)
                     /gene="pdxB"
                     /locus_tag="ACHKJF_002015"
     CDS             complement(29545..30681)
                     /gene="pdxB"
                     /locus_tag="ACHKJF_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP0949375.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30780..31775
                     /gene="flk"
                     /locus_tag="ACHKJF_002016"
     CDS             30780..31775
                     /gene="flk"
                     /locus_tag="ACHKJF_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP0949376.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31772..32950)
                     /locus_tag="ACHKJF_002017"
     CDS             complement(31772..32950)
                     /locus_tag="ACHKJF_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949377.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33234..34454)
                     /gene="fabB"
                     /locus_tag="ACHKJF_002018"
     CDS             complement(33234..34454)
                     /gene="fabB"
                     /locus_tag="ACHKJF_002018"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP0949378.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34613..36619
                     /gene="mnmC"
                     /locus_tag="ACHKJF_002019"
     CDS             34613..36619
                     /gene="mnmC"
                     /locus_tag="ACHKJF_002019"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP0949379.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSHTSTLPVYLVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT         9073 a         9993 c         9099 g         8513 t
ORIGIN      
        1 taacaatagt ttcacacctt gcccccattg cgacacttcg gaaatcatca cgctgttaaa
       61 ctctccgtaa tgcggaaata tcatcatcgc gtttcattag tgaattctta aatgaggcat
      121 tttcacacaa ttatcttaca gataaaaaaa ccagacttac aattaagaat cagaacaatt
      181 cactatataa cattgcatat aaagcatata cacctcacta ttttgtcatt attaagttat
      241 taacagcaca atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac
      301 cgtcctgggg gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc
      361 aaaaaaggtc aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt
      421 atgacatccg tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg
      481 tactgaaact gaacatcggc aacccagccc cgttcggttt tgacgcgcca gatgaaatcc
      541 tcgttgacgt gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaaggtcttt
      601 actccgcgcg taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg
      661 tggaagatat ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat
      721 tgctgaacag cggggacgaa atgttggttc ctgcaccaga ttacccacta tggaccgcgg
      781 cggtttcgct ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt
      841 tcccggacct cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta
      901 tcaacccaaa taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg
      961 agattgcacg tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct
     1021 acgacgatgc tgagcatcac tcaattgcgc cactggcacc tgacctgctg accattacct
     1081 ttaacggact gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga
     1141 acgggccgaa aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc
     1201 gcctgtgtgc taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga
     1261 gcatcagtga atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg
     1321 aactgatcaa cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt
     1381 tcccgaaaat cgacgccaaa cgctttaaca ttcacgacga tcagaaaatg gtgctggatt
     1441 tcctgttgca ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg
     1501 atcacttccg cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt
     1561 tcgcgcgttt cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc
     1621 atccggcagt gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat
     1681 gaaacagagc catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct
     1741 catgcgcaac gtgcggacgg aaaatgtttc cgaacacagt ttgcaggtag cgatggtcgc
     1801 ccatgcgctg gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat
     1861 cgctttactg gcgatgtacc acgatgcctc tgaagtgctc accggcgatc tccctactcc
     1921 ggtgaaatac ttcaattcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca
     1981 gcaaaaactg gtcgatatgg ttccggaaga gctgcaggat atctttgcgc cgttaattga
     2041 cgagcatgca tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc
     2101 atatctgaaa tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac
     2161 gcgactggaa gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt
     2221 atttgttccc agcttccatc tttcgctcga tgagattagc caggattcac cgctataagc
     2281 agccggagtc tgcgtcgcat caggcaataa gcgccggatg cgacatcagg ctcttgtcaa
     2341 aacggaaaca gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt
     2401 acccccaact tcacaaagtc actgaagctg taatttcccg gacctaaaac cagtgtgtta
     2461 acaggtgaag aaaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgcg
     2521 aatggataag gcgacacacc catcgttttg gcagcagcca gcgcaatcgg agccatcaac
     2581 accgccgtcg cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacaa
     2641 cccagcatca tatgtggccc ataaccgccg ccaatgtcca tcagcccttt caccgccagc
     2701 gcgacacctc ccgttttctg taatgccaca gcaaacggca tcatcccaac gatcaaaata
     2761 atgctcggcc agtgaatgga tttataggcg ctttcggcat cgatacagcg gaatttcccc
     2821 atcagcaagc aggcgatgat agcggcaaca ggattaggaa tttcatcggt cagcattaac
     2881 gccaccatca acaccagaca gaaaatggca tggggtgcct gactgtgtgc gggcgatgct
     2941 tcactcactt cttctggtaa gttcagcgcc acaaagtcac gcccctgttt tgccagcata
     3001 ccgatcagtt tccagttacc aacaaccagg atgatatcgc ccagcagcag aggctcatcc
     3061 gccagcgaac cttccagcgc cacgccattg cgctttagcc ccaccacatt cagtccgtag
     3121 cgggtacgaa aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat
     3181 gaaatctctg ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc
     3241 atcggctcca gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata
     3301 tcaataagca aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg
     3361 cgacgaaaac gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat
     3421 ttgagatcgt ccagccgttg accaatcatt ggcgatccgg ggcgaatagc cagacgtcgc
     3481 gcacgcccgg tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct
     3541 tcgcgctgcg gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa
     3601 atacccagca ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga
     3661 tagccttcac gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc
     3721 atcatgccgc taatcagccc ggcaaaactc aacggcatca tcaggcgcga cggcgacgtt
     3781 tgcatacgca tggcaacgct taacaccacg ggaataaaga tagcgacaac gccggttgaa
     3841 ctcataaacg cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca
     3901 ctgttgcccg caactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc
     3961 aaaccatcgc caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg
     4021 gaaaatactt cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa
     4081 gcgaccgcat ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat
     4141 gaaagaaccc aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact
     4201 tgattctgcc ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact
     4261 gaataataac atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga
     4321 ccaaatcgac ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc
     4381 aacccgccgc caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct
     4441 gcggcgcaag ccccagcagc tgcgcgccta acagatacgc atcaggttct ggttttccgc
     4501 gcttcactcg ctcagcggtt acaaacacct ctggtgcggg aagcccagct attttatggc
     4561 gcgctcgcgc taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat
     4621 tcaaatgact gagtaaggcg attgcccccg gaagcgcggt aataccttcg gtttccgtgg
     4681 cctcgatttg ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca
     4741 taaaatggcg cagagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg
     4801 gcgctaaccc atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat
     4861 ccaccagcgt tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag
     4921 gcgttgataa tttgatttat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc
     4981 cacacattaa gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccgtga
     5041 gttccggcgt caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg
     5101 aggtaacgtg cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg
     5161 gattgctggt cctgcaaatt agaccaggaa acatgcaacg catgatacat ctccataatg
     5221 tcgatgatag tgcggcaggt ttcttctttc agctcgccaa actcgcgatc cagttcacgc
     5281 atctgtaatc cataaccacg ctcaatgatt gtttgcaggc gacggtaacg ttcagcattt
     5341 gccggatcga gcataatcat catcttgtac tggttagaca aaataagacg ttgcgcgttg
     5401 gtcatttcca ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt
     5461 atgccttctt ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc
     5521 cagttgtttg aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc
     5581 aacgggtgcc aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt
     5641 aaggattgca gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat
     5701 gggtaaactt aaggcgaaca gggcagtggc aactgccggc ccaagctgac cgcccagggc
     5761 aatctgccag cagagagtaa atacagcgat cggcggcata aaacggatcg cataacgcgt
     5821 catcttgata acgcgatttt cgacaaagac cggggcaagg cgtttttcca gcggccacgt
     5881 ctttgagtaa tgctgtcccc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg
     5941 cgttgacatg cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca
     6001 actcagcggg acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta
     6061 gcagcctgaa ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc
     6121 cattggctga aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa
     6181 aaaatttgca gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat
     6241 ggctccctga cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt
     6301 agtactggtt ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa
     6361 tggtgaagag tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa
     6421 atggaaaatg gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga
     6481 agcgctcaac tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct
     6541 gactgctatc ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat
     6601 cgatgagtct gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc
     6661 ggctcacctg atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa
     6721 cgttgctgta tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc
     6781 cctgccgtac aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag
     6841 ccacttctat gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa
     6901 catcatcacc tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg
     6961 cgttgacacc tctatgggcc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg
     7021 tgatatcgat ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc
     7081 aatcaacaaa ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga
     7141 ctgccgctat gttgaagaca actacgcaac gaaagaagac gcgaagcgcg caatggacgt
     7201 ttactgccac cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct
     7261 ggacgctgtt gtattcaccg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc
     7321 tctgggcaaa ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg
     7381 tttcggcaaa tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac
     7441 caacgaagaa ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc
     7501 cagctcagct ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa
     7561 accgtgtccc gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc
     7621 agccttggcg tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct
     7681 atcgctcagc cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg
     7741 aactcttcca ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt
     7801 tccagcaatc agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc
     7861 aaagacgctg aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc
     7921 cagtctctga actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct
     7981 cagggcactg acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc
     8041 ggcggtgcca aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt
     8101 gatgaacagg ggcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct
     8161 aaagtaaaca atgttgatcc ggcgaagctg caagaatcca gcccgctgcc ggttctcggc
     8221 gctgtgccgt ggagctttga tctgatcgcg actcgtgcga tcgatatggc tcgccacctg
     8281 aatgcgacca tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc
     8341 tgcgcacgca gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact
     8401 tccgcagacc gtcctgacgt gctggtggcc gcgtgcctgg cagccatgaa cggcgtagaa
     8461 atcggtgccc tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc
     8521 gaacgtgctt tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc
     8581 tctctgagcc tgcagagctt caacctggaa gttccggttg acgatcacga gcgtatcgag
     8641 aaagttcagg aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc
     8701 acttctgagc gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactt
     8761 gcgcgcaaag cgggtaaacg tatcgttctg ccagaaggtg acgaaccgcg taccgttaaa
     8821 gcagccgcta tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca
     8881 gagatcaacc gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt
     8941 gatccagaag tggttcgcga aagctatgtt gctcgtctgg tcgaactgcg taagaacaaa
     9001 ggcatgaccg aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg
     9061 atgctggaac aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac
     9121 accatccgtc cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc
     9181 gtgttcttca tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg
     9241 gatccgaccg cagaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc
     9301 ttcggtatcg aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt
     9361 agcgacgtag aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg
     9421 atgatcgacg gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa
     9481 gcgccgaact ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc
     9541 ggtaacacca cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg
     9601 ctgcagggta tgcgcaagcc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc
     9661 gtctacacca tcgcgctgac tgcgattcag tctgcacagc agcagtaacc tcgtcatcat
     9721 ccgcagcttt gcgctgcgga tatctgaacc ggaaatagtc actatttccg gttttttatt
     9781 ctcttaattt gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt
     9841 gcgccatctc actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg
     9901 tccgcaatca ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt
     9961 atcttttttg ttgctatttt aaccagcctt gccacctggg tagttccggt ggggatgttt
    10021 gacagtcagg aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat
    10081 ccacactcat ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag
    10141 ctgttcacga cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc
    10201 tcaggatcga aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc
    10261 gcgtttggca ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc
    10321 catacccgcg ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc
    10381 ggcgcggtat ttggtatggg ggaagaggcc gtcgcctttg ccattatcat cgcaccgcta
    10441 atggtccggc tgggctatga cagtattacc accgtcctgg tgacctatat tgccacgcaa
    10501 atcggttttg ccagttcgtg gatgaacccg ttttgtgtgg tcgttgctca ggggattgcc
    10561 ggcgttccgg tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg
    10621 attggcctga tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca
    10681 cgcgtgcatg agtccgaccg cttctttcgt gaaaagcagg ctgatgttga acaacgtccg
    10741 tttacctttg gtgactggct ggtattgatt gtcctgaccg ccgtaatggt ctgggtgatt
    10801 tggggcgtga tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg
    10861 ggtctggtga ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc
    10921 atggcttcat cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt
    10981 ttcgccaaag ggattttgct gctggtcggt aatggtgaag cgggtgatgc cagcgtgtta
    11041 aataccatcc tcaacagcat tgccaatgcc attagcggtc tggacaatgc ggtcgcggcc
    11101 tggtttatgt tgctcttcca ggcagtattt aatttcttcg tgacgtccgg ttctggtcag
    11161 gcggcgttaa ccatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag
    11221 gttaccgtgc tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca
    11281 gcttcgttaa tggcgacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg
    11341 ggcgcgaccc tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag
    11401 ttgatgggct accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc
    11461 gtttcagtct ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc
    11521 agcgagtctg gagtgaactc atcgcagcgc gcggtgattt cttccggcgt cagccagcaa
    11581 acttcactga cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac
    11641 gcgccccaga cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg
    11701 acaccggcaa tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca
    11761 tcggcctgaa ccacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc
    11821 tcggtacgac gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca
    11881 cgatgacgca gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc
    11941 tcttcattga caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg
    12001 aaaccttctt tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta
    12061 atcgcgacct gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta
    12121 tccagcatgc cataactcgc cggattaccg cctttcggaa tactcaccga gccggggttg
    12181 aagtggaaaa tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc
    12241 acatcgttct ggtttaaagc aggcagattt tccgggccaa aaagatgacc atgcgtcaaa
    12301 aacagacgtt gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc
    12361 agcatttgat ccacttcgct gtcgcagttg ccgcgcacag cgataacctt atgtgccact
    12421 tcattaagca gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg
    12481 ccgtgattca acacgtcgcc aaggatcacc agccactggg caccgctttg ggcaaacaac
    12541 tccagaacac gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc
    12601 atcactcact cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca
    12661 gccagattgc ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa
    12721 tgtcgcataa tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat
    12781 cattagcgcc agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag
    12841 tgctaacaaa tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt
    12901 tagtggcgct tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg
    12961 catcagatcg ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc
    13021 aagcggataa atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat
    13081 ggcagaagat tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg
    13141 accgtaacct tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat
    13201 atgaaacgac aggccttttt cctgcaacgc cacccaggca gataacacat aaggggagaa
    13261 aaagtgggca tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa
    13321 gggggctggc tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt
    13381 aagtcgccgc gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg
    13441 atcgatctct atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa
    13501 gcagagcttg attatcgctt gattaaggta gacctgggga aaggcggtca gtttcgtccg
    13561 gaatttttgc gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc
    13621 gatggcggcg aaccgctaag cctctttgag tctggtgcca ttttgttgta tctggctgag
    13681 aaaacaggac tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta
    13741 ttctggcagg taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca
    13801 gccccccaaa ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac
    13861 catgtactaa acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt
    13921 gcggatattg cctgctggcc gtgggttaat gcctggactc gccagcgaat tgacctcgca
    13981 atgtatccgg cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag
    14041 gcactgctaa aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc
    14101 gtgtattatt tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta
    14161 ttattcgtat aaagaacctt cgtttacgca cgtttattgg aattaaggac gaagaaatta
    14221 acaaccgtca ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca
    14281 ccagcgaaga tattaatgac gcgctgaact atcgcaccgt caccaaaaac attattcagc
    14341 atgtagagaa taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg
    14401 cacgtgaaca tcactgggtg acttatgctg aagtggagat cgataaacta cacgcgctgc
    14461 gctacgccga ttcggtatcc atgaccttga gctggcagcg ttaaccgcca tatcgggagg
    14521 ctgcatgaac atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg
    14581 tttgctggag ctgggccatc aaatcacggt agtgacgcgt aacccacaga aagccagttc
    14641 cgttctcggc cctcgggtga cgctatggca agggctagcc gatcaaagca acctcaacgg
    14701 cgttgatgcg gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacga
    14761 gcaaaaagag cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat
    14821 taatgccagc gacacgccac cgtcggtact catttccggc tcagcaacgg gctattatgg
    14881 cgacttaggt gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttacccataa
    14941 actctgcgcc cgctgggaag aaattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg
    15001 cctgctgcgt accggcgtag tgctggcacc ggatggcggt attctcggta aaatgctgcc
    15061 gccgtttcgt cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat
    15121 tcatatcgat gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc
    15181 atttaatatg gtttcgccct acccgatacg caatgaacaa tttgcccatg cgctcggtca
    15241 tgcgctgcat cgcccggcta ttttgcgcgt ccccgcaacc gccattcggc tgttaatggg
    15301 cgaatcttca gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc
    15361 gggttttgcg tttcgttggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg
    15421 tggtttactg caaacatccg ccagttaact cccggtgtta cagggttagt ggctttgcgc
    15481 gataagatcg tctggtgaaa gtcgggtcac catcataact aactctcggt ctaaacctct
    15541 atccagcatc tcctgagcaa tacgcagggc ttcttcgcgt ttgccaagaa ttaatccatc
    15601 attccgccct tcttcacgta atctgtcagc aatggtcatc agtttctcct tttcctgtgg
    15661 tgcgcgttct gctatctcac caataaatgc acggaaacgc tgggcatctc ccgtttgtaa
    15721 tacataatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa ctagcagcga
    15781 aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct gaattaactc
    15841 caacagcgcc attttgcgat gttgcataat ctcgtcatcc ggcaccacgg taatatccac
    15901 caacggaaaa gccgatgaat atattttacg ggcaatcgca ggctcggcaa attcatcaag
    15961 ccagcagagt gaataaggat aaggacttct gcaaccatga taaaacagca ttgggatcac
    16021 caatggaagc tctttatagc ctgcatccag atggttttgc atcgccgcga tggaatagcg
    16081 catcatgcga aaagccatta attcttccgg cttactttgg tgctctatca ctacataaat
    16141 ataacccact ccctcctgcg ttttcacaga ccacaagagg tcggaataat attgccgcag
    16201 gtcttcatca ataaaactgt ttggttccag tttaagcgtc gttaaatcac acagtttgcg
    16261 cagcggcgcg ggaagatgaa tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa
    16321 agatttaaat accgcatcat gcggcgtgga agttgttgat attgtcatgg cgttccgtca
    16381 cccctcaaat ttaagatgac gcgacaataa ccacgattaa cacccaatgc agcaggcagt
    16441 ttttatcttt ggacagcgaa ttcagaagta tttttagcgg actgcaactt ttacatttca
    16501 gcggaagctg actcgcaaat tccccatgaa aatactccgg cagcgttagc caccggagag
    16561 gaaattattt cagcgatccc ttaaggaact gttgcagacg agggctttgc gggttgccaa
    16621 ataactgctc cggcgcgccc tcttcttcta ttttcccctg atggaggaaa atgacgtgag
    16681 tagaaacatg gcgagcaaag cccatttcgt gagtcactac caccatggtt ttcccctctt
    16741 ctgccagttg ctgcataata cgcaacactt cgcctaccag ttcaggatca agcgccgagg
    16801 taggttcatc aaacagtaaa acttccggtt ccatcgccag cgcccgcgcg atagaaacac
    16861 gctgttgctg accgccggaa agatgcaccg gatatttccc ctgcgcacgt tcgtctatcc
    16921 cgactttcgc cagatacttc accgcccgct cgcgcgcttc ctgcttgctc aggcccaaca
    16981 cctgaatcgg cgcttccatg acgttttcca gcaccgtcat atggctccag agattgaagt
    17041 gctggaatac catcgtcagg cgtgtgcgca gtaagcgcag ttgattttta tcggcgactt
    17101 tgagttgacc atctttgtcg cgcaccagat tgatcgtctg gccactgacc acgatcgatc
    17161 cttcgctcgg tttttcgagg aagttaatgc agcgcagaaa ggtacttttc cccgatcccg
    17221 acgatccgat gatgcttatt acatctccgg cattcgcttg cagtgacacc cctttcagca
    17281 cttcgtgttc gccatagcgt ttgtgcaaat cgataacgtt taatttattc tcggacatcg
    17341 tattctcagt gcgttgaaga aggtttcaca tgctgcaacc agcgtttttc cgctctgcga
    17401 aagaggctga tcaggacata agagatgatt aaatagagca ccgcggcaat gccgaaggcg
    17461 gtaaaaggtt gatacgtggc ggcgttaata tcgcgggcta ttttcagcag atccggcacc
    17521 gtggccgtaa acgccagcgc agtagagtgc agcatcagga tcacttcatt gctgtatgcc
    17581 ggtaacgcaa tacgcagcgc agaaggcaaa ataatgcagc gatacatttt aaaggtcgag
    17641 aagccatagg ctctggcggc ttcaatttcc ccatgcggaa ccgaacggat tgccccagca
    17701 aaaatctcgg tagtgtaagc gcaggtgtta agcgtcagcg ccagcacggt acagttcagg
    17761 ccactgcgga agaaagcgtt aaggaattcg gttcccttaa caatctcaag cgtgtacatg
    17821 ccggaataga acaccagcaa ctgaacatac agcggcgtac cgcgaaaaat ataggtaaat
    17881 aaccagattg gaaactggat gtatttatta ctggagacac gaccaatcgc cagaaacagc
    17941 gccaggactc cgcctatcac taccgacaaa ataagcagcc acagagtgat cgccacacca
    18001 gtaaagcgat aaccgtcggt ccacagcagc ggtttccagt attcatgtaa gatttcgatc
    18061 acaggtcagc cctcttcaca cccacggagt agcggcgctc aaggaacagc agcacaccat
    18121 tggaaacagt ggtgaaaacc aggtaaatca cgccacagac gatggcgaaa tagaacggtt
    18181 cccaggtact tttgcctgcc agttgggtgg ctttgaccac atcttccagg ccgagtaacg
    18241 aaaccagtgc ggtagatttg aggatcacct gccagttgtt gccaatgcct ggcagggcgt
    18301 aacgcatcat cgccggaaac atgatccgcc gaaacacttg cccacgagta aaaccgaacg
    18361 ccgtcgccgc ctctatatgt cctttcggca ctgccataaa agcaccacgg aacgtttcgg
    18421 taaaataagc accgtagata aaaccgagag tgataatacc agcgaccatc ggatcgatat
    18481 caatctgccc gacgcccatc gcctccgtca ccgtgtttag cgcaatctgc aaaccgtaga
    18541 aaatcagcag catcaacact aagtccggca cgccacgaat cagcgtggtg tacccttcga
    18601 aaatcagccc cgaaagccga ttttgcgaga gcttaccgcc agcgccaatt aaaccgatga
    18661 ttacagcgag cactacagag ctgatagcca gctccagcgt gacgagcgca ccctgtaaaa
    18721 taacacctga aaacccatac aacatgcagc ctgtcctgtc gtgtgtggtg aattaccgcc
    18781 tcttgtctcc ctccggttac ccgaagggag cgggctttct ttcaccgatg gcgattagcc
    18841 accataaaca tcaaaatcga agtacttttt cgctaatttc tcgtaagtac cgtcagcgcg
    18901 catttcggca aaggctttgt tcagtgcttc gcgcagttcg ttatcttctt tacgcaggcc
    18961 catgccggtc cctacgccaa acagtttttc atctttaaca gacgggccac cgaatttgta
    19021 atctttaccg acaggttgtt tgaggaaacc ttcgctggca gcgacctcat cctggaacgc
    19081 ggcatcaata cgtccggcag tcaggtcaga ataaatgttg tcctgcccct gatacgagac
    19141 gatttcaatg ccttttggtg cccaatgttc attaccgaac gtctcctggg tggtgccctg
    19201 caatacgcct acccgtttgc ctttcagcga ctcgactgtc ggctgaatgt cagaattttt
    19261 cgccaccacc aaacgagaat cggcagcgta cagtttgtcg gtgaaggcta tttcttgctg
    19321 acgtttttcc gtaatggaaa gcgatgacat gatggcgtca atcttcttcg cttttaagga
    19381 cgggattaac gcatccagcg gattttcgac aaacgtacat tgcgtattga tgcgtttgca
    19441 taattccttt gccagatcga tatcgaagcc aaccagttcg ccttgtgaat tttttgattc
    19501 aaatggcgca taggtcgggt cggtaccgat gcggatgttt tgcggaatcg cagcaaacgc
    19561 cgcagttgcg ctggagaagg ccagaaccag agagagcgat agcaccagtt ttttcataat
    19621 gtcctcaact gacagtcttt attataggga atatttacag gtttgttaca cttatcgtgc
    19681 cataaaattg accatcaagg caaaatatcc cgccccgtta gatgtattat ttttcattaa
    19741 aaatgcttaa gtatgcattt caatgcacca cgatagtgca acaaacctgg cgtgcaccat
    19801 gatgatcatt ccatcaggta cagcttccca gcgacgtatc agtcaccgta gacattaaag
    19861 tcgaaatact ttttcgccat cttgtcgtag gtgccgtcct gacgcagctc gccaagcgcc
    19921 ttattgaagg cagccgtcag ttcagcatca tctttacgta gccctacacc ggtgccatca
    19981 ccgaagtatt ttttgtcttt tactgatgag ccagcaaagg cgaaatcttt accagcaggt
    20041 tgcttgagga atccttcgct ggcagcaact tcatcttgta acgcagcatc cagacgtcct
    20101 gcagccagat cggaatagac caaatcctgg ttggcatagg ccaccacatc cacgcctttg
    20161 ctacgccagg tctcgttagc gtaagcttcc tgggttgatc cctgcagcac accaacatgt
    20221 ttacctttca gtgaatccag cgttggctga atcggtgaac ctttggcagc aatcaaacga
    20281 gaatctgcgg cgtacagctt gtcggagaag gcaatctcct gctgacgttt atcggtaatg
    20341 gaaagcgacg aaataatggc gtcgattttt ttcgctttca gtgaggggat cagcgcgtca
    20401 aagtcactgg caacccaggt acatttcacc tgcatccgtt tgcacatctc gttaccgaga
    20461 tcgatatcaa agccaacaaa atcaccttta gcatctttcg acgagaacgg cgcataggtg
    20521 gtatcggttc cgatacgtac cgtctccggt agcgccgcat agctggaagc cgctgcggag
    20581 agaccgacta acaaagacag agcgagaatc gacttcttca tacataaccc tcaagtgaaa
    20641 atggcttttt tatgttgtgt attgtgttgt gtgtttgcag gctctttcat gcaggtctta
    20701 tgccatcttg acgacaacag taacattcaa cgttaaatat gttaataaga cgttgcatta
    20761 ttgtcctgaa gttgaagata gcaggtatag cggttgaatc gcagcgtttc gattgaatgg
    20821 cagaaacaaa atgtcgagga tttgatcgcg gttgcaaaat tgccctgaaa cagggcaaca
    20881 gcggagttat gcgccctgcc agcgggcaaa gagatcttca ggaagggtta tcgcaaactg
    20941 gtcaagaaca cgattaaccg tctgatttat cacatcatca agggattgcg ggcgatgata
    21001 aaacgccgga acgggaggca taatcaccgc accgatttct gccgcctgag tcattaaacg
    21061 cagatggcct aagtgcaatg gtgtttcacg cacgcagagc accaacgggc gacgctcttt
    21121 cagcaccaca tctgccgcac gggtcagtaa gccatcagta tagctatgga caatgccgga
    21181 aagggttttg attgaacagg gtaaaatcac catccctagc gtctggaaag aaccggaaga
    21241 gatgctggcg gcaatatcgc gcgcatcgtg cgtgacatca gctaatgcct gcacttcgcg
    21301 cagagaaaaa tccgtttcga gggataaggt ctggcgcgct gcctggctca tcaccagatg
    21361 cgtttcgata tctgtgacat cgcgcagaac ctgtaataag cgcacgccat aaatcgcgcc
    21421 gctggcaccg ctgatgccta caatgagtcg tttcataaat agttgccctg tcagacttgc
    21481 aggcagactt tgcaggattt cggcgggagt tgcaagtcag ggtgccagac cggcaccctc
    21541 agcgaaggca tcatccttcg ttatgcattt cgagattttc cacttcgttc tgacgttgca
    21601 ctgctttggc gtcatcatta cgtaacgtat cgaggaaatc gaggtagccc tgatcaacat
    21661 ctttggtgac gtagacgccg ttgaacaccg agcattcaaa ctgctggata tccggatttt
    21721 cagcgcgaac ggcgtcgatc agatcgttca gatcctggaa aatcaacccg tcagcaccga
    21781 tgatctggcg aatttcatca acttcgcgac cgtgagcgat cagttccgtg gcgctcggca
    21841 tatcaatacc ataaacgttc gggaagcgaa tttccggtgc cgcagaagcg aggtacactt
    21901 tcttcgctcc ggcttcgcgt gccatctcga taatctgctc agaagtggtg ccacggacga
    21961 tggagtcgtc gaccagcagg acgtttttat cgcggaactc ggcgcggttg gcattcagtt
    22021 tacggcgcac ggacttacga cgcagctgct ggcccggcat gataaaggtg cggccaacat
    22081 agcggttttt aacgaagccc tggcggtacg gtttgcccag aatacgagca atttccagcg
    22141 cgatatcaca cgaggtttct gggatcggga tcaccacgtc gatatccaga tcttcccatt
    22201 cgcgggcaat tttctcgccc agtttcgtgc ccatattcac acgcgcgctg taaacggaaa
    22261 ttttgtcgat gaacgagtcc gggcgagcaa agtatacata ctcaaacagg cacggattgc
    22321 tgaccggatt gtcagcacat tgacgggtaa acaactgccc ttcttcagtg atgtaaatcg
    22381 cttcgcccgg cgcgacgtca cgcaggaaat caaagcccag cgtatcgagc gctacgcttt
    22441 cggaagcgac catatattct gtacggttct cgtcaatatc acgttttccc agtaccagcg
    22501 gacgaatccc gtttggatcg cggaaagcaa ccataccgtg gccgataatc atcgccacac
    22561 aggcatatgc gccgcggatt aagcggtttg tggcagcaat ggcagcgaaa atattgtcgg
    22621 cttccagcgg gtagtggcgg aagttgtcca gctcgctggc gaagatatta agcagaattt
    22681 ccgagtcgga agtggtgttg atgtggcggc gtttttcttc aaacagtttt ttacgcaact
    22741 cgtgagcgtt ggtcagattg ccgttgtggg caagcgtaat gccatacgga gagttaacgt
    22801 aaaacggctg cgcttcagag gcgctggagc tgccagccgt agggtaacgc acatgaccaa
    22861 tgcccatatt gccctgcaaa cgctgcatat ggcgagcttc aaatacatcg ctcaccagcc
    22921 cgttcgcttt acgcaaacgg aagcagttat tggcatctat ggtgatgatg ccggcggcat
    22981 cctgaccgcg atgctgaagc accgttaagg catcataaat cgactggtta accggcataa
    23041 caccggcgat accgacaata ccgcacatac gtctttttcc tcgttaagcc acatctcaga
    23101 gcacttacgc tctgggcaag aaacttgacg agctttgcag ataatcaaaa aaccatctga
    23161 tgataaaact gaactgcggg atcagctgtg atttgctcca gtcttcgctt ttcgacaccc
    23221 cggtaaagga gtcgagaaag aagagaatgg cggcaacaat caacacaccg cgcaacgcac
    23281 cgaaacagac gcccagcacc cgatcggtgc ctgacaaccc cgttttctcc accaactggc
    23341 ctatcacgaa gttcacgata gcaccaacga tcagggtagc gataaacagt accgcgatgg
    23401 caatcccatt tcgaaccagt tcgtcttcaa agcccgtaaa ccagactgac aggtaagtgt
    23461 agtaatgact ggcaacaaag aaagcacaac cccatgtcac cagcgataac gcttcacgaa
    23521 caaagccgcg gatcaggcta accagagagg aaaaagcaat caccgcgatt atggcgtaat
    23581 caatccagac catatgtgtc ccacgatttt acgccctgtc atcctgttcg gggcgcattc
    23641 taacagaaaa agaaaacgtt tgcgtaggga tttccttccc acgcatcaat aaaaatggcg
    23701 ctgaaaaaat attcaacgcc atcgactttt tatgcctttg cggcatcgtg caatgcgtgt
    23761 cggatgcggc gtaaacgcct tatccgacct acggttctaa cactgcccag gcctgataag
    23821 acgcgccagc gtcgcatcag gcaagactgt attaattcgg cgtatagccc attaccacgc
    23881 cacttaagcc agaaagttgc ttcaactcac ccagcgaacc tttcagctta tctttcgagg
    23941 catccggccc aaccagaata cgggtaattt taccctgcac tggcgtggat ggcgacgtat
    24001 aaacccgata accggcaccg cgcagcttac cgacaatctc attcacttta tcggcatttt
    24061 tcagcgcacc cagttgcaca acataggctt tacccgttgg cgctgctttt tcttccacga
    24121 ccggcttcgg ttctggcttc ggcgcaggtg gtgcttcaac cttcggttta ggcggctcca
    24181 ccggtttcgg cttcggtggg gcgaccggtg caggttccgg ttcaaactcg gtgttattgg
    24241 ctgcaatagt ggtcggatcg agcgacggtg ctgcggcatc acctgcccgc acctcttccg
    24301 ctgcgccttc cggcggctgc gtcggcaacg cctgggtggc ggctggcatc atatcaggct
    24361 catcgcgatc gcccgctttc ggcaccagcg ggatagccgc gaactcatcc tgataatgtt
    24421 ttttctgccc gtccagcagc cctggaagta caatcacccc cagcgccacc agcacgatcg
    24481 tgcccactaa ccgattctga aacttacttg ccaccgcttc tcctcgcgtc aatcacttcc
    24541 atgacatgtg cgacagtgtg gaaagaacca cacaccagca cggtgtcttc cgctttagcg
    24601 tccgccattg cggcatccca tgcctgcgca acgctatcaa atgatttgcc gttacccaaa
    24661 tgctcaagca gttgttctgc cgtggcaccg cgcggccctt ccagtggcgc acaataccag
    24721 tcatcaacca cgcttttcaa ccaggccaga gttccggcaa tatctttatc atgtagcata
    24781 ccgataaccg ccagcacgcg cccgtttttc ggtagcgctt tcatacgccc ggtgagatat
    24841 tccgccgcat gtggattatg cgcgacatca aaaataacgc gtggcgactc gctcacaatc
    24901 tggaaacgtc ccggcaaaat tgcgctggca atcccgtcgc gaatggcatt ttcactgact
    24961 tccagcccgc tggcacgcag tgccgccagc gctgttgcgg cattcggttg cgggacaagc
    25021 ggcaacggca gattttccag cgtgccgtga gcatcgctaa acgcccagtc atgatcggtg
    25081 acggaatagt tccactcaac gccccgacgt tgtaacagtg cacctttttc ctgcgccaca
    25141 tcagcaatgg tagaaggcat ttccggctca ccgacaattg ccggttttgc gctgcgaaag
    25201 atgcctgctt tctcgcgacc aatactttcg cgatctggtc ccagccagtc ggtatgatcc
    25261 agcgcaatgc tgattactac agccacatcg gcatcgacaa tattggttgc gtccagacga
    25321 ccgcccagcc ctacttccag aatcactacg tcaagttgtg cctgtttgaa cagccacaac
    25381 gccgacagcg taccgtactc gaaataggtc agggaaatat caccgcgtgc cgattcaatc
    25441 tccgcaaaag aggcggtgtg ggccgattcc ggcaattcct gcccctgcac acgtacgcgc
    25501 tcggtataac gcaccagatg cggtgaactg tagacgccca ctttgtaccc tgccgccatc
    25561 agaatcgact ccagcgtacg gcaggtggtg cctttgccat tcgtacccgc aacggtaaac
    25621 acaaatggcg ctggtttcag gacgccaaga cgcgccgcga cctggctcac gcgctcaagg
    25681 ccgagatcga tagttttact gtgcaggttt tccagataag aaagccacga agccagaggc
    25741 gacgcggctt gaggagtgcg tttgataatc atggtatccg ctgattcgtt acggtgagaa
    25801 tagcaaaagg gcagagccag tggccctgcc cttatcagtt atcaggcctc aggttcctga
    25861 tccggtaccg ggggtaccac tacgccttca cgcggcgctt caggattcgg cgctggcaga
    25921 ttcatcaact tcgccagaat gctcgccagt ttcaggcgca tttccggacg acggacgatc
    25981 atgtcgatcg cgcctttctc gatcaggaat tcactgcgct ggaatcctgg cggcagtttt
    26041 tcgcgaacgg tctgttcgat aacacgcgga ccggcaaagc cgattaacgc tttcggttca
    26101 gcgatgttga gatcgcccag catggcgaaa cttgcagaaa caccgcccat cgtcgggtcg
    26161 gtcagcacgg agatgtacgg caagccgcgc tcctgcattt ttgccagtgc cgcagaggtt
    26221 ttcgccatct gcatcagcga catcagtgct tcctgcatac gtgcgccacc agaggcggag
    26281 aagcagatca gcgggcagtt atcttccagc gcctgctcaa cggcacgcac gaaacgtgca
    26341 cccacaacag accccattga accgcccata aaggcgaact cgaatgccgc agcgacaacc
    26401 ggcattccat acagagtgcc tttcatcacc accagcgcat ctttttcgcc agtttctttc
    26461 tgcgcagatg ccagacggtc tttatacttc ttggagtcac gaaacttcag cacatctttc
    26521 ggctcaagct cgctacccag ctccacaagg cttccttcat ctaacaggct atgcaggcga
    26581 ttacgcgctg tcatacgcat gtgatggtca cacttcggac agacctcaag attacgttcc
    26641 agctcagcgc ggtataaaac ctgaccgcag ctatcacact tagtccacac cccttcagga
    26701 atgctcgcct tgcgggtggg agtaatgttg cttttaattc gttcaatcca gctcattagg
    26761 gacctttctg tctgaacctg gttcgatgcc agttttatct ttggggacgc ataatgccat
    26821 ttttgccccc aacagaccat gaatgttgca cattaaaaca taacagcccg aaactttgga
    26881 taaaaaagtg gtcgaaccgc ggagttactt tttattttgc ggcgcgtgcc gcagcgcgtt
    26941 tgtgacggat tatttcgatg acgcccggca aaatagaaac cacaataatc ccgacgatca
    27001 gcagcttaag gttatcctga accatcggga ttgtaccgaa gaaatagcct gcgtaggtaa
    27061 aaagcagtac ccacaacagt gcgccgatca cgttataagc ggcgaaatga cggtacgaca
    27121 tgtggcccat tcccgcaaca aacggggcga acgttctgac gatcggcaca aaacgggcga
    27181 gaataatcgt tttgccgcca tgtttctcat aaaactgatg ggttttgtcg agataactgc
    27241 gacggaaaat tttcgaattg ggattactga acaacttttc accgaacaac cgcccgatag
    27301 tgtaattgac cgcgtcaccc acaatcgcgg cgatcagcat cagcaccacc atcatatgga
    27361 cgttgagatc gttggtttcc agtgatgcca acgctccggc aacaaacagc aatgaatcac
    27421 ccggtaaaaa cggcgttacc accagaccgg tttcacagaa caaaattaag aacaaaatgg
    27481 cataaaccca gacgccgtac tccgcgacca gctctgccag atgcacgtca atgtgcagga
    27541 taaaatcgat gaggaaataa atcaggtcca tatttgccta tgccttgtac tcgtcatctt
    27601 tcaggctgta actacgttgg ctacagcctg aaatgttccg agcattattc tcgttagtcc
    27661 gccagaaata gcgggcccat tggcggtttt ggaagatcat accggtcagg gtaatccacc
    27721 gcgaccagat acagcccttc cgcttttgcc gttgctgccg ccagcgttct gtcctttgcc
    27781 gccagcaact ctgctatcca gctctccggc tggttgtggg caccgacttc catcaggctg
    27841 ccgacaatat tcctgaccat atgatgtaca aaggcattcg ctttgatatc taccaccaca
    27901 taaggaccgt gacgcgtgac attaatatgc ataacgttgc gccacggggt tcgggactgg
    27961 cactgcaccg cacggaacga ggtgaaatca ttctcgccca gcaagcattg cgcagcccga
    28021 tgcatccgtt cagcgtccag cggttcgtaa aaatgggtta cccctttact cagtaccgcc
    28081 gggcgcagcc gatgattgta gatgatgtag cgataacggc gagccgtggc gctaaatcgg
    28141 gcatgaaaat catcaggtac agctttaacc caacgcacgg cgatgtcacc aggtaaattc
    28201 gcatttacgc ccaaggtcca cgccgcgtct ttgcgctgcg cggtcgtttc gaaatgcact
    28261 acctgcccgg taccgtgtac accagcgtca gtacgcccgg cgcagaagac ggtaatgggt
    28321 tcgttcgcca cctgggagag cgccttttcc agcttctcct gcacactgcg gacttcattc
    28381 tgccgttgcc agccgtaata cttactgccg tcgtactcaa tgcccagcgc aattttataa
    28441 actggcggtt gttgctggtc ggacattagt acagatactc ctgcaccagt ttctcggcga
    28501 tttttactgc catcagcgcg ccgccaaagc gaacgttatc ggccaccgac cagaactgga
    28561 cttgctccgg cataccgtag tcattacgca cgcagccaac agaaagatgc ggcgtacccg
    28621 aagcatcccc tacctgagtc gggaattcgt tctcttcaga gagcacaata tcttcgccct
    28681 gagcaaacgc atcacacgct tcttctgccg ccagcggacg cagtgcttca aagttgacca
    28741 tctgggcatg accgtagaat accggtgcct ggacgacgct tgccgaaatc atcagccctt
    28801 cgtcctgcag gattttgcgt acttcgtcaa cgatacgacg ttcttcacgc acgctacctt
    28861 cgctatccgg caacaacggc agcatattga acgccagttg acgaccgaag aaatcttctt
    28921 cgtcaatcgg aatgccgttg agcaatttcg cactctgccc cgctaacgca tcgaccgctt
    28981 ttttgccctg ggcggaggct gaaatcaggc tggtaacact gatacgcgac aaaccgccct
    29041 gatcgattaa cggtttcagt gccgccagca gctggctggt cagactgtct ggtacggcga
    29101 tgacattccg gttgcggtaa tcggtcagta caaacgggtt tacttccggc accaccagcg
    29161 gtacgtcggg ttcgagagca aacagtccgc tgctgtcgat caccaggcaa cctgagttgg
    29221 tcgcttcttc aacccaggca gcggtagctt ctttgcctgc gacaaaaaat gccagctgcg
    29281 cctgcgtcca gtcgaattca gcggcatcct gcacggtgat tgtcttacca ccaaagcgca
    29341 gttgttcgcc tgcgctttcg ttacgtgcca gtgcataaat ttccccaacc gggaactgac
    29401 gttcagccag cgtttcaagc agggcttcgc ccacagcgcc agttgcgccc aggacggcaa
    29461 tgttccagcc ttcagacatg gtggtttact ccagaaatag caaagctccc tgccaatgtt
    29521 acagcagaga gcatgaagaa gagattaacg tgccggatga tgaacggcgt taaaacccag
    29581 tttacacagc aatgatgccg cactggcgtc atcacaaatt acatacagag atgaccattc
    29641 acggcgctca agatagtttt tgcgcagttt atcgaactca cccggtatcc cggcgacttt
    29701 acgcagcggt gcgtcatcgc ggcgcacatc atacaccaaa tgcaccagcc ttttcagcgt
    29761 cggttgatcg agcgggccat gcagcgtaat gcgaccaaac tctggcgcgg gcagtaatgt
    29821 atccagcgca acgtgctgtt catgcccaat aaacttgcta taggcttcaa acacttgcgt
    29881 agtaccgcgt gctttacctt ccagggtata acctgcgata tgcggcgtgc cgatatccac
    29941 ttttgtcagc aactccacgt taagttccgg ttcgccttcc cagacatcca gcactacgct
    30001 taacttctgg ccttcattca ggcaggtcag caacgcagta ttatcgacga ctgcgccacg
    30061 gcaggcgtta atcagaatcg ctcccggctt caggctacgg atcagttttt catccgccag
    30121 atgcagcgtt ttgtacggcc catctttgaa gagtggcgta tggaaagtca gaatatccgc
    30181 atgctgaact aattcatcca gcgagcggaa atcaccctca tccccacggt cagcgcgagg
    30241 cggatcgcaa agcaatgttt taatccctaa cgcttccagt cgcgcctgca atcgacgtcc
    30301 aacgttacca acgcccacga tccccaccgt acggtcgtgc agtgaaaatc catcgcgttc
    30361 ggcaagcatc agtagtgagg agaaaacata ttccaccacc gcaatcgcat tacagccagg
    30421 tgcagcggaa aaaccaattc ccgcctgctt taaccatgct tcatcgacat ggtcggtccc
    30481 cgcggtggca gtgccaacaa atttaatggg ttttcctgcc agcaaagatt cattcacttt
    30541 cgtgaccgaa cgcaccatca gcgcatccgc gtctgccagt tgagcgacgg ggattggacg
    30601 cccgggaacc gcggtcacct cacccaaacg gctaaataag tcgcgggcat aaggcatatt
    30661 ttcatcaaca aggattttca cgtttgtgtt acctgtatga gacgagagtt aaccggacaa
    30721 gtgtgccata atctcgcggc caggcatact tgcgaagatt tcaggtataa ggatacgtaa
    30781 tgatacaacc tatttccggc cctcctcctg ggcaaccacc aggtcaggga gataacctgc
    30841 cgtctggcgc gggcaatcag cctttatcca gtcagcaacg tacttcgctg gaaagcttaa
    30901 tgacgaaagt gacctcactg acgcaacagc aaagagcaga actgtgggcg ggtatcaggc
    30961 acgatattgg tctgtcggga gattcaccgc tgctttcgcg tcacttccct gccgctgaac
    31021 acaacctggc gcaacgtctg ctggccgcgc aaaaaagcca ttctgcccgc cagcttttag
    31081 cgcaattagg ggagtattta cgtctgggga ataatcgtca ggcggtcacg gattatatcc
    31141 gtcataactt tggtcagacg ccgctgaatc agctctcacc ggagcaatta aaaaccattc
    31201 tcaccctgtt gcaggaaggg aagatggtta ttccgcaacc gcagcagcgc gaggcgaccg
    31261 accgtccttt attaccggcg gagcacaatg cgctcaaaca gctggtgacc aaacttgcgg
    31321 cggcaacggg ggaacccagc aagcagatct ggcaatcgat gctggaacta tccggggtga
    31381 aagatggcga gttaattcca gcgaaactgt ttaaccatct ggtgacctgg ttgcaggcgc
    31441 gccagacgct aagccagcaa aatacgccga cactggaatc actacagatg gcgctaaaac
    31501 aacctttaga tgccagtgaa ctggcggcgt tatcggcata tatccagcaa aaatatggcc
    31561 tttctgcgca atcatcgctt tcttctgccc aggccgagga tattcttaat cagctttatc
    31621 aacggcgggt taaagggatt gatccgcgtg atatgcaacc gctgcttaat ccttttccac
    31681 cgatgatgga cacgttgcaa aatatggcaa cgcgtcccgc gctgtggata ctgttagtcg
    31741 cgattatcct gatgctggtc tggctggttc gttagccccg acgaaatgac agtatcgtga
    31801 caataattcc cagcaccgca gagatcgccc cggcaagaaa taccgaagag taaccaaacg
    31861 tggtcgccag cattcccgcc agcggcccgg agacgccgag ggcgatatcc tgaaacgcgg
    31921 cgtaaccgcc cagtgcggtg ccgcgaactt gtgaggggac gcgtttaacc acctccacgc
    31981 ccagcgcagg aaagataagc gaacatccgg ctccggttaa cgccgcgccc gctaatgcga
    32041 cccacgcacc tggggcttgc cagagcagca acaagcccac cgtttctaca agcagagaga
    32101 caatcgccac tttcacgccg ccaaaacggt ccggcatcca gccaaacatg acgcgcatca
    32161 cgacaaatgc gccgccaaac gcggtaagag taaagcccgc catcgcccat cctttgctgg
    32221 caaagtagag cgaaacgaaa gtcccgataa ccgcaaaacc aacgccttgt agtgccagcc
    32281 ctaaccctgg tttccagata aacccgacaa cgctccacag cgatggacgt tctcccgcca
    32341 gggccggtac tttgcgcact gtgccgttac aggcccacgc cagtaagggt aataccattg
    32401 tggtgatcgc cagtgcggca aaaccgtaat ggctatgaat caacaggcca agcggagcac
    32461 caacagcgag ggcaccgtaa atcgccattc cattccatga catcactttg ccagagtgtt
    32521 ttggccctac gatgcctaac ccccaggtca gagcgcctgt cagtaactgg ctttcaccaa
    32581 acccaagaat caaacgcccg atgaccaaca gggcaaattt gaacggtgcg gagacaggca
    32641 aaatcgccgc cagcagcaac gcgccgccag ccagaccaca agctaacatc ccctgaagcg
    32701 ccgaacgttt tgcaccatat tgatcggcca gtcgcccggc atagccacgc gtcagcaccg
    32761 tagccagaaa ctgaatcccg acggcaatcc cgaccatggt attgccatag cccagatcat
    32821 gatgaacaaa cagcgggata accggcaacg gcagccctac ggtcatgtag gtgagaaaaa
    32881 ccgcaaaagc gatgcggaag agcgaaaaat tggcagaaga tcgtgtttcg gtttggctta
    32941 cagcagtcat gcattactcc agaatgcagc gcaaggcgag gagtatcccc gtctcatctc
    33001 tctggtttca gggttacagt gcgttggcag gatttaacgc gtacgtcttt tcagaaggaa
    33061 atcgacaaag cgggaagttt gcctggaact ggcggcgatt gtcaatgatg tgaaaaaggg
    33121 aaccatcagg ttcccttttg cgttagtgcc ggaggccgca ttggcgcgta acgtcggatg
    33181 cgacgctggc gcgtcttatc cgacctactc cgactactcc gacctactgc gaattaatct
    33241 ttcagcttgc gcattaccag cgtggcgttg gtgccgccga agccgaagct gttagacata
    33301 acggtggtca gttcgcgatc ggtcgtttcg gtcacgatgt tcagacctgc agcctgctcg
    33361 tccagctctt caatgttgat gctcggggcg ataaagccgt gttccagcat cagcagagag
    33421 tagatagctt cctgtacgcc agcagcgccc agagagtgac cggtcatggc tttggttgca
    33481 gaaatcgccg ggctcttatc gccgaacact tcacggatag ctgccagttc tttcacgtcg
    33541 ccaaccggag tcgaagtacc gtgagagttc aggtaatcga ttggggtatc aacgccgtgc
    33601 atcgccatct tcatgcagcg tactgcgcct tcgccagacg gagcaaccat gtctgcacca
    33661 tcagaggttg cgccgtagcc aacgatttca gcatagatgt gagcaccacg cgccagcgcg
    33721 tgttccagct cttcaaccac taccataccg ccgccgccag cgataacgaa accgtcacgg
    33781 tgagcgtcgt aagtacggga ggctttttcc ggggtgtcgt tgtatttagt agacagcgca
    33841 cccatcgcgt cgaattcgca ggccatttcc cagcacagct cttcgccgcc gccagcaaac
    33901 acgatgtcct gtttgcccag ttggatctgc tctactgcgt taccgataca gtgtgcggaa
    33961 gtcgcacacg cggagctgat ggagtagtta acgccatgaa ttttaaacgg cgtggcgagg
    34021 caggcagaaa cgccggatgc catcgctttg gtgaccacat acgggccaac cgctttcagg
    34081 ccgcgcgggc cgcgcattgc gtcagcgccg aacacctgga aacgcgggga accgccgcca
    34141 gaacctgcaa tcaggccaac gcgcgggtta ttctggtaag cttccggaga aaggcccgca
    34201 tctgcgattg cctgctccat agaaaggaat gcataaatgg atgcgtcgct cataaagcgc
    34261 acaactttgc ggtcaatgag gccagtggta tccagtttta cgttgcccca gacgtggcta
    34321 cgcatgccgg aatccttcag ctcctgagag aaagtgatcc ctgaacgtcc ttcacgcaga
    34381 gatgccagga cttcctgctg gttattaccg atgctggaaa caatgcccag gccagtaatc
    34441 actgcacgtt tcattcaata cctctgtaag tcgcacatag agtaagtttc gaatgcacaa
    34501 tagcgtacac ttgtacgccg aacaagtccg atcagccatt taatagagaa atttgcgcag
    34561 ccttacacac atcgctaaga tcgagccacc gcctgtaaga cgagtaactt acgtgaaaca
    34621 ctactccata caacctgcca acctcgaatt taatgctgag ggtacacctg tttcccgaga
    34681 ttttgacgat gtctattttt ccaacgataa cgggctggaa gagacgcgtt atgtttttct
    34741 gggaggcaac caattagagg tacgctttcc tgagcatcca catcctctgt ttgtggtagc
    34801 agagagcggc ttcggcaccg gattaaactt cctgacgcta tggcaggcat ttgatcagtt
    34861 tcgcgaagcg catccgcaag cgcaattaca acgcttacat ttcattagtt ttgagaaatt
    34921 tcccctcacc cgtgcggatt tagccttagc gcatcaacac tggccggaac tggctccgtg
    34981 ggcagaacaa cttcaggcgc agtggccaat gcccttgccc ggttgccatc gtttattgct
    35041 cgatgaaggc cgcgtgacgc tggatttatg gtttggcgat attaacgaac tgaccagcca
    35101 actggacgat tcgctaaatc aaaaagtaga tgcctggttt ctggacggct ttgcgccagc
    35161 gaaaaacccg gatatgtgga cgcaaaatct gtttaacgcc atggcaaggt tggcgcgtcc
    35221 gggcggcacg ctggcgacat ttacgtctgc cggttttgtc cgccgcggtt tgcaggacgc
    35281 cggattcacg atgcaaaaac gtaagggctt tgggcgcaaa cgggaaatgc tttgcggggt
    35341 gatggaacag acattaccgc tcccctgctc cgcgccgtgg tttaaccgca cgggcagcag
    35401 caaacgggaa gcggcgatta tcggcggtgg tattgccagc gcgttgttgt cgctggcgct
    35461 attacggcgc ggctggcagg taacgcttta ttgcgcggat gaggcccccg cactgggtgc
    35521 ttccggcaat cgccaggggg cgctgtatcc gttattaagc aaacacgatg aggcgctaaa
    35581 ccgctttttc tctaatgcgt ttacttttgc tcgtcggttt tacgaccaat tacccgttaa
    35641 atttgatcat gactggtgcg gcgtcacgca gttaggctgg aatgagaaaa gccagcataa
    35701 aatcgcacag atgttgtcaa tggatttacc cgcagaactg gctgtagccg ttgaggcaaa
    35761 tgcggttgaa caaattacgg gcgttgcgac aaattgcagc ggcattactt atccgcaagg
    35821 tggttggctg tgcccagcag aactgacccg taatgtgctg gaactggcgc aacagcaggg
    35881 tttgcagatt tattatcaat atcagttaca gaatttatcc cgtaaggatg actgttggtt
    35941 gttgaatttt gcaggagatc agcaagcaac acacagcgta gtggtactgg cgaacgggca
    36001 tcaaatcagc cgattcagcc atacgtcgac tctcccggtg tatttggttg ccgggcaggt
    36061 cagccatatt ccgacaacgc cggaattggc agagctgaag caggtgctgt gctatgacgg
    36121 ttatctcacg ccacaaaatc cggcgaatca acatcattgt attggtgcca gttatcatcg
    36181 cggcagcgaa gatacggcgt acagtgagga cgatcagcag cagaatcgcc agcggttgat
    36241 tgattgtttc ccgcaggcac agtgggcaaa agaggttgat gtcagtgata aagaggcgcg
    36301 ctgcggtgtg cgttgtgcca cccgcgatca tctgccaatg gtaggcaatg ttcccgatta
    36361 tgaggcaaca ctcgtggaat atgcgtcgtt ggcggagcag aaagatgagg cggtaagcgc
    36421 gccggttttt gacgatctct ttatgtttgc ggctttaggt tctcgcggtt tgtgttctgc
    36481 cccgctgtgt gccgagattc tggcggcgca gatgagcgac gaaccgattc cgatggatgc
    36541 cagtacgctg gcggcgttaa acccgaatcg gttatgggtg cggaaattgt tgaagggtaa
    36601 agcggttaag gcggggtaat ctgctctcgc agtgtttgcc ggatgcggcg tgaacgcctt
    36661 atccgaccta cgtgtgac
//
LOCUS       DAZGXZ010000039        36367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000039 DAZGXZ010000000
VERSION     DAZGXZ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.039"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1084)
                     /locus_tag="ACHKJF_002020"
     CDS             complement(<1..1084)
                     /locus_tag="ACHKJF_002020"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP0949380.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERSIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAF"
     gene            complement(1305..1379)
                     /gene="tnaC"
                     /locus_tag="ACHKJF_002021"
     CDS             complement(1305..1379)
                     /gene="tnaC"
                     /locus_tag="ACHKJF_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP0949381.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            complement(1622..2986)
                     /gene="mnmE"
                     /locus_tag="ACHKJF_002022"
     CDS             complement(1622..2986)
                     /gene="mnmE"
                     /locus_tag="ACHKJF_002022"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP0949382.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(3092..4738)
                     /gene="yidC"
                     /locus_tag="ACHKJF_002023"
     CDS             complement(3092..4738)
                     /gene="yidC"
                     /locus_tag="ACHKJF_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP0949383.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(4741..4998)
                     /gene="yidD"
                     /locus_tag="ACHKJF_002024"
     CDS             complement(4741..4998)
                     /gene="yidD"
                     /locus_tag="ACHKJF_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP0949384.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(4962..5321)
                     /gene="rnpA"
                     /locus_tag="ACHKJF_002025"
     CDS             complement(4962..5321)
                     /gene="rnpA"
                     /locus_tag="ACHKJF_002025"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP0949385.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(5338..5478)
                     /gene="rpmH"
                     /locus_tag="ACHKJF_002026"
     CDS             complement(5338..5478)
                     /gene="rpmH"
                     /locus_tag="ACHKJF_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP0949386.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(5708..5788)
                     /locus_tag="ACHKJF_002027"
     CDS             complement(5708..5788)
                     /locus_tag="ACHKJF_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949387.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            6085..7488
                     /gene="dnaA"
                     /locus_tag="ACHKJF_002028"
     CDS             6085..7488
                     /gene="dnaA"
                     /locus_tag="ACHKJF_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP0949388.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7493..8593
                     /gene="dnaN"
                     /locus_tag="ACHKJF_002029"
     CDS             7493..8593
                     /gene="dnaN"
                     /locus_tag="ACHKJF_002029"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP0949389.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            8593..9666
                     /gene="recF"
                     /locus_tag="ACHKJF_002030"
     CDS             8593..9666
                     /gene="recF"
                     /locus_tag="ACHKJF_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP0949390.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            9695..12109
                     /gene="gyrB"
                     /locus_tag="ACHKJF_002031"
     CDS             9695..12109
                     /gene="gyrB"
                     /locus_tag="ACHKJF_002031"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP0949391.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            12349..12747
                     /gene="yidB"
                     /locus_tag="ACHKJF_002032"
     CDS             12349..12747
                     /gene="yidB"
                     /locus_tag="ACHKJF_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP0949392.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            12862..13674
                     /gene="yidA"
                     /locus_tag="ACHKJF_002033"
     CDS             12862..13674
                     /gene="yidA"
                     /locus_tag="ACHKJF_002033"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP0949393.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(13720..14376)
                     /gene="yidX"
                     /locus_tag="ACHKJF_002034"
     CDS             complement(13720..14376)
                     /gene="yidX"
                     /locus_tag="ACHKJF_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP0949394.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            14654..15343
                     /gene="dgoR"
                     /locus_tag="ACHKJF_002035"
     CDS             14654..15343
                     /gene="dgoR"
                     /locus_tag="ACHKJF_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP0949395.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15340..16218
                     /gene="dgoK"
                     /locus_tag="ACHKJF_002036"
     CDS             15340..16218
                     /gene="dgoK"
                     /locus_tag="ACHKJF_002036"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP0949396.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AALAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            16202..16819
                     /gene="dgoA"
                     /locus_tag="ACHKJF_002037"
     CDS             16202..16819
                     /gene="dgoA"
                     /locus_tag="ACHKJF_002037"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP0949397.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            16816..17964
                     /gene="dgoD"
                     /locus_tag="ACHKJF_002038"
     CDS             16816..17964
                     /gene="dgoD"
                     /locus_tag="ACHKJF_002038"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP0949398.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            18045..19382
                     /gene="dgoT"
                     /locus_tag="ACHKJF_002039"
     CDS             18045..19382
                     /gene="dgoT"
                     /locus_tag="ACHKJF_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949399.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(19343..20443)
                     /gene="cbrA"
                     /locus_tag="ACHKJF_002040"
     CDS             complement(19343..20443)
                     /gene="cbrA"
                     /locus_tag="ACHKJF_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP0949400.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHISARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNEITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVLLKQPEKSNAAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            20544..21758
                     /gene="yidR"
                     /locus_tag="ACHKJF_002041"
     CDS             20544..21758
                     /gene="yidR"
                     /locus_tag="ACHKJF_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP0949401.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIAE
                     SGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPHDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(21760..22092)
                     /gene="yidQ"
                     /locus_tag="ACHKJF_002042"
     CDS             complement(21760..22092)
                     /gene="yidQ"
                     /locus_tag="ACHKJF_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0949402.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    22332..22404
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            22398..22811
                     /gene="ibpA"
                     /locus_tag="ACHKJF_002043"
     CDS             22398..22811
                     /gene="ibpA"
                     /locus_tag="ACHKJF_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP0949403.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENTQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            22923..23351
                     /gene="ibpB"
                     /locus_tag="ACHKJF_002044"
     CDS             22923..23351
                     /gene="ibpB"
                     /locus_tag="ACHKJF_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP0949404.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            23548..25209
                     /gene="yidE"
                     /locus_tag="ACHKJF_002045"
     CDS             23548..25209
                     /gene="yidE"
                     /locus_tag="ACHKJF_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP0949405.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(25206..25922)
                     /gene="yidP"
                     /locus_tag="ACHKJF_002046"
     CDS             complement(25206..25922)
                     /gene="yidP"
                     /locus_tag="ACHKJF_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0949406.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            26218..27834
                     /locus_tag="ACHKJF_002047"
     CDS             26218..27834
                     /locus_tag="ACHKJF_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0949407.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            27834..28648
                     /locus_tag="ACHKJF_002048"
                     /pseudo
     CDS             27834..28648
                     /locus_tag="ACHKJF_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28645..29538)
                     /gene="yidL"
                     /locus_tag="ACHKJF_002049"
     CDS             complement(28645..29538)
                     /gene="yidL"
                     /locus_tag="ACHKJF_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0949408.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            29705..31420
                     /gene="yidK"
                     /locus_tag="ACHKJF_002050"
     CDS             29705..31420
                     /gene="yidK"
                     /locus_tag="ACHKJF_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP0949409.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKIDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            31417..32910
                     /gene="yidJ"
                     /locus_tag="ACHKJF_002051"
     CDS             31417..32910
                     /gene="yidJ"
                     /locus_tag="ACHKJF_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0949410.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(32957..33406)
                     /gene="yidI"
                     /locus_tag="ACHKJF_002052"
     CDS             complement(32957..33406)
                     /gene="yidI"
                     /locus_tag="ACHKJF_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP0949411.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            33515..33862
                     /gene="yidH"
                     /locus_tag="ACHKJF_002053"
     CDS             33515..33862
                     /gene="yidH"
                     /locus_tag="ACHKJF_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP0949412.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            33852..34214
                     /gene="yidG"
                     /locus_tag="ACHKJF_002054"
     CDS             33852..34214
                     /gene="yidG"
                     /locus_tag="ACHKJF_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP0949413.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            34211..34708
                     /gene="yidF"
                     /locus_tag="ACHKJF_002055"
     CDS             34211..34708
                     /gene="yidF"
                     /locus_tag="ACHKJF_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP0949414.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(34716..35900)
                     /gene="emrD"
                     /locus_tag="ACHKJF_002056"
     CDS             complement(34716..35900)
                     /gene="emrD"
                     /locus_tag="ACHKJF_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP0949415.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            35982..36056
                     /gene="ysdE"
                     /locus_tag="ACHKJF_002057"
     CDS             35982..36056
                     /gene="ysdE"
                     /locus_tag="ACHKJF_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP0949416.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
BASE COUNT         8608 a         9292 c         9660 g         8807 t
ORIGIN      
        1 cgaatgccgc gtgaccgccc gcctgctggc agacaacgcc aatctcttcc agaccatcga
       61 ccagatactg tacctgcgcg atacgataag ccagccagtc gagattcatg ccgtcataca
      121 gacctaccgc cagacgctcc atcgcgccgc cttccaggcc gccatatgtc gggaaccctt
      181 cctgcaccac gcaaagggtt ctgcactcgg tgtacacatc aaagaagctg tcgtctttca
      241 tgcacagcaa gccgcccatc ggtaccatcg catctttctt ggcggacatc gccagcatat
      301 cggcatattt gtaggtttcg cgggtgatct gctcgatggt ccagtctttg tattctgctt
      361 cacgctgctt gatgaaatag gcgttttcag caaagcgtgc ggagtccatt accaccggaa
      421 tatcgtattt cttcgcgatg ctgtacatcg cttttaagtt tgccagtgaa accggctgac
      481 cacctgcaga gttactggtg atggttgcaa cgatatacgg cacgttattc gggccaactt
      541 cttcaatact gcgttctaat ccttcgaggt caaagttgcc tttaaagtcg taacgcacgc
      601 ccgtatcgaa ggcttctttg atatagacgt tacgcacggt acagccgtta atctggctat
      661 ggccctgcgt ggtatcaaag aaatagttag agaatgccac cattttgctg cgatccaggc
      721 ctttttcctg ctcgcgtttt ttaatcagta ccggaatata gatttgctct gcgccacggc
      781 cctggtgagt cggaatagta tattgataac caaagatatt tttcactgac tcggctaacg
      841 catagtagct gcggctgccg ctgtaggctt cgtcgccgcg catcatcgct gcctgcatac
      901 tttgggttac cgccccggtg ccgctgtcgg tcagtaaatc gataaacaca tcttcgctat
      961 ccagcaggaa cgggttcata ccggatttaa taattgcttc ttcacgataa gcgcgagtag
     1021 tacgttttac tggctcaata acacgaatgc ggaacggttc agggagatgt ttaaagtttt
     1081 ccattacata atccttcatt tattttaatt acagtgatcc ctgtgaatat tacatctgca
     1141 atatccatta attcgccatg tatatataca agcaccatca tttactaaaa taagtaaata
     1201 aacgagagat ggcctttgca aaaggcaaaa ttaagatata gcaaactaat cgttcacatt
     1261 gaatctaatc ggtttggctc tggtgatggc tacagaaggg taaattaagg gcggtgatcg
     1321 acaatcttat tgtcgatatt gaaccatttt gaggtcacac atatatgtaa gatattcata
     1381 atgcacttat cctcgaaaga cacgaggcta cagtataata ctcaagaatt ttgtctattc
     1441 taaaattgtt taaatgtgaa tcgaatcaca atcgttcggg gagcaatact aaatgctttt
     1501 ttttaagatt ataaattacg taagaatttt aatgtttaaa aaaagggttg aggaataaca
     1561 ggagtgctaa caatgaagcg aagccgcatc tgacagtcag aatacggctt cgtaagcgcg
     1621 gttacttacc aatacagaag ctggagaaaa tccgccccag caggtcgtct gaagtaaatt
     1681 ccccggtgat ttcgcttaag ttctgctgtg ccaggcgcaa ctcttccgcc agcagttcac
     1741 ccgcccaggc acctaacagt tgcgctttac cttgctgcaa atgctccgct gcctgttcca
     1801 gcgcctgtag gtggcgacga cgcgccagga agccgccttc catgttggtg tcaaagccca
     1861 tgctctgttt gagatggtta cgcagcacct ccacgccttc acctgtcctt gccgagagac
     1921 gaattaacgc gtgaccgttc acttcactca ttcccagcgt ttcgccggtg atatcggctt
     1981 tattgcgcac cacggtgatc ggcagtttcg ctggcagacg ggcgataaat tccggccaga
     2041 tctctgccgg atccacggcg tctgttgtgg tgccatcgac cataaacagc acgcggtcgg
     2101 cctgttcaat ttcctgccac gcgcgctcga taccaatacg ttccacttcg tcactggctt
     2161 cacgtagccc agcggtatcg atgatatgca gcggcattcc gtcaatgtgg atatgctcac
     2221 gcagcacgtc acgtgtggtt ccggcgatat cagttacgat tgctgcttca cggcccgcca
     2281 gcgcgtttaa caggctcgat ttaccggcgt taggacgtcc ggcaatcacc accttcatcc
     2341 cttcgcgcaa caaactaccc tgacgtgctt cagcacgcac tgcatcgaga tcggcaatca
     2401 cgtcattaag ctgggcttca attttgccgt cggagaggaa atcgatctct tcatcaggga
     2461 aatcaattgc cgcttcgacg taaatgcgca agtgggtgag ggcttccacc agatggttga
     2521 cccgtgcgga gaatgcgcct tgcagcgagt taagtgccga acgggccgcc tgttccgaac
     2581 tggcgtcgat aagatcggca atcgcctcgg cctgggctaa gtcgagttta tcgttaagaa
     2641 acgcgcgttc ggaaaactca ccagggcgag caatccgcag gccgggaatg gtcagaatgc
     2701 gttttaacag caggtcgagg atcaccggac cgccatgacc ttgcagttcc agcacatctt
     2761 cgccggtgaa cgagttcggg ccagggaacc atagcgcaat cccctgatcg agcacgctgc
     2821 cgtcggcgtc tttaaacgga agataatcgg cgtagcgcgg cttaggtagt ttacccagca
     2881 cggtttcggc aacttcacgg gctttgaagc cggagatgcg caggatgcca acgccgccac
     2941 gtcccggagg cgtggcctgg gctacgatag tgtcattatc gctcatgatg ttcctgttgc
     3001 tttgtgtggc ggatgcgcgg tgcttatccg ccctacgaaa agaaaaaagg cggtcaactg
     3061 accgccctta ttttagcgaa aactcaccga atcaggattt tttcttctcg cggctatgca
     3121 ggccacgttt ttccagacca cggtaaatca gctgctgctg aataatggtt accaggttgc
     3181 tgacgatata gtacagcacc agacctgacg ggaaccacag gaagaacacg gtgaagatga
     3241 ccggcataaa ggtcatgatc ttctgctgca tcgggtcggt cactgtggtc ggcgacatct
     3301 tctgaatgaa gaacatcgtt acgcccatca ggatcggcag gatgtagtac gggtcctgtg
     3361 ccgacaggtc gtggatccac agtgcaaacg gtgcctgacg cagttcaacg gaacccatca
     3421 gcatgtagta caacgccagg aagattggca tctggatcag cagcgggaag cagccgccca
     3481 gcgggttaac cttctcagct ttgtacagcg ccatcatttc ctggctgata cgctgtttgt
     3541 catcacccag acgctcacgc attgcctgaa tcttcggctg cagcatacgc atcttcgcca
     3601 tggaggtgta ctgcgctttg gtcagcgggt acatgatgcc acgaacgata aaggtgatga
     3661 tgataatgga gaagccccag ttacccacaa agctatggat ccatttcagc agtttgaaca
     3721 gcggctgaga gatgaaccac aaccaaccgt aatcaacggt cagatccagg tgcggagcaa
     3781 cagctgccat tttgtcctgg atttccgggc caacccacag ggtgctgttc atcgcgccag
     3841 tctgaccagg ctgaaccagt accggctgag atttatagcc gatagcggcg atgccgttac
     3901 ccagattagc ggtatagaag ttgttggtac cgtcgttatg cgggatccac gccgtcgcga
     3961 aatactgttg cagcatcgcc acccaaccac ctttcgaaga gatgttcagg ttttcgttat
     4021 cggcaatggt atcgaacttg tatttctcat acttctcgtc aggcgtggag tacgccgcgc
     4081 cacggaaggt gtgcagtgcg aagttgctgc ttccggtatc gagatgcggt ggcagagtga
     4141 tggattgctt caactgacca aacgaggaga tttccagcgg tttctcgcca gcgttctgca
     4201 cgttgtagtt gacgttgaca gcgtaatcac cacgtttcag gacaaacgtt ttggtaaacg
     4261 tgttgcctgc cgcgtcggta tacgtcatcg gcacctgcag ttcgttttga ccttcagcca
     4321 gcacataagc gtctttttca acgttataca gcggacgcgg gccgttagcc gggttatccg
     4381 ggccatcacg accggtcaga ccgctctgtg cctgataaat aaactgcggt gaagtttcca
     4441 gcagctggaa cggctgggta gagttcagct ctttcgggta agcaggcagc agagcttgct
     4501 caacatcacc accacgggtg ttgatggtca gatcaagcac gtcggtctta accgagatca
     4561 gtttcccctg gccactggcc ggtacgccct ggtcggcggc gctacccgct gcggtggtcg
     4621 ttgtctgcgt ggtctgttgg gcctgaggtt gcgggttttt atcctgctcc caggcttgcc
     4681 agatcatgaa agacacgaac agcaaagcga tgactaaaag attgcgttgc gaatccatcg
     4741 ttagtgttct ctggtatcaa atggtccggg cgggacggga tcgtcaccac cagggtgtaa
     4801 agggtggcat tttaatacgc gtttcaccgt caaccaactg ccttttatca ctccaaacct
     4861 gcgcaatgcc tcaattccgt agcttgaaca ggttggagtg aaacgacaat gcggcccgag
     4921 tagcggacta atcaggcgtt gatagacccg aatgagggct atcaggaccc gcgagccagg
     4981 cgacagtggc ggcgccataa tttttccaac gcttccgaga gagcacggtt atcgaggtcg
     5041 gcaacccctt ttttcgccac caccacgaaa tccatagccg ggagttcatg ttggcgcaga
     5101 cggaagcttt cacgcgtcag acgtttaatc cgattgcgtt catgggcgcg tcgaacgttt
     5161 ttcttggcga ctgtaagacc gatacgggga tgccccagcg aattcaggcg gccgagaatg
     5221 gtaatttgcg gcgtgccagc ccgttgtggc tgctggaaga cgaatgtgaa ttgactggga
     5281 gttaacaagc gtaactccct gggaaatgcg agcttaacca ctcaggggtt agctttatta
     5341 cttagaaacg gtcagacgag cgcggccttt agcacgacga cgtgccagaa cctgacgacc
     5401 atttttagta gccatacgag cacggaagcc gtgagaacgg ttgcgcttca gtacagacgg
     5461 ttgaaaagtg cgtttcatgg cgatttctac ctaaacttga ataaattcaa tggctttatt
     5521 ggatatccgc cgaaaaatga aacgatggac accgaagcca tgggtgatta aagaggccgg
     5581 attgtaataa ttgtacactc cggagtcaat tctctttcct tatttaccgc gcttttccgc
     5641 accttttcgc agggaaaatg tacgacctca caccagtgga aaccagcatg gcgcgccggg
     5701 tggaggatta tacgggctga tgggtaaagc gcaaggatcg tcctggatct ttattagatc
     5761 gattaagcca atttttgtct atggtcatta aattttccaa tatgcggcgt aaatcgtgcc
     5821 cgcctcgcgg caggatcgtt tacacttagc gagttctgga aagtcctgtg gataaatcgg
     5881 gaaaatctgt gagaaacaga agatctcttg cgcagtttag gctatgatcc gcggtcccga
     5941 tcgttttgca ggatcttgat cgggcatata accgcagaca gcggttcgtg cgtcaccctc
     6001 aagcaggggc ttttcgacgt acgtcaacaa tcatgaatgt ttcagcctta gtcattatcg
     6061 acttttgttc gagtggagtc cgccgtgtca ctttcgcttt ggcagcagtg tcttgcccga
     6121 ttgcaggatg agttaccagc cacagaattc agtatgtgga tacgcccatt gcaggcggaa
     6181 ctgagcgata acacgctggc cctgtacgcg ccaaaccgtt ttgtcctcga ttgggtacgg
     6241 gacaagtacc ttaataatat caatggactg ctaaccagtt tctgcggagc ggatgcccca
     6301 cagctgcgtt ttgaagtcgg caccaaaccg gtgacgcaaa cgccacaagc ggcagtgacg
     6361 agcaacgtcg cggcccctgc acaggtggcg caaacgcagc cgcaacgtgc tgcgccttct
     6421 acgcgctcag gttgggataa cgtcccggcc ccggcagaac cgacctatcg ttctaacgta
     6481 aacgtcaaac acacgtttga taacttcgtt gaaggtaaat ctaaccaact ggcgcgcgcg
     6541 gcggctcgcc aggtggcgga taaccctggc ggtgcctata acccgttgtt cctttatggc
     6601 ggcacgggtc tgggtaaaac tcacctgctg catgcggtgg gtaacggcat tatggcgcgc
     6661 aagccgaatg ccaaagtggt ttatatgcac tccgagcgct ttgttcagga catggttaaa
     6721 gccctgcaaa acaacgcgat cgaagagttt aaacgctact accgttccgt agatgcactg
     6781 ctgatcgacg atattcagtt ttttgctaat aaagaacgat ctcaggaaga gtttttccat
     6841 accttcaacg ccctgctgga aggtaatcaa cagatcattc tcacctcgga tcgctatccg
     6901 aaagagatca acggcgttga ggatcgtttg aaatcccgct tcggttgggg actgactgtg
     6961 gcgatcgaac cgccagagct ggaaacccgt gtggcgatcc tgatgaaaaa ggccgacgaa
     7021 aacgacattc gtttgccggg tgaagtggcg ttctttatcg ccaagcgtct acgatctaac
     7081 gtacgtgagc tggaaggggc gctgaaccgc gtcattgcca acgccaactt taccggaagg
     7141 gcgatcacca tcgacttcgt gcgtgaggcg ctgcgcgact tgctggcatt gcaggaaaaa
     7201 ctggtcacca tcgacaatat tcagaagacg gtggcggagt actacaagat caaagttgcg
     7261 gatctccttt ccaagcgtcg atcccgctcg gtggcgcgtc cgcgccagat ggcgatggcg
     7321 ctggcgaaag agctgactaa ccacagtctg ccggagattg gcgatgcgtt tggtggtcgt
     7381 gaccacacga cggtgcttca tgcctgccgt aagatcgagc agttgcgtga agagagccac
     7441 gatatcaaag aagatttttc aaatttaatc agaacattgt catcgtaaac ctatgaaatt
     7501 taccgtagaa cgtgagcatt tattaaaacc gctacaacag gtgagcggtc cgttaggtgg
     7561 tcgtcctacg ctaccgattc tcggtaatct gctgttacag gttgctgacg gtacgttgtc
     7621 gctgaccggt actgatctcg agatggaaat ggtggcacgc gttgcgctgg ttcagccaca
     7681 cgagccagga gcgacgaccg ttccggcgcg caaattcttt gatatctgcc gtggtctgcc
     7741 tgaaggcgcg gaaattgccg tgcagctgga aggtgagcgg atgctggtgc gctccgggcg
     7801 tagccgtttt tcgctgtcta ccctgccagc ggcggatttc ccgaacctcg atgactggca
     7861 gagtgaagtc gaatttaccc tgccgcaggc aacgatgaag cgtctgattg aagcgaccca
     7921 gttttcgatg gcgcatcagg acgttcgcta ttacttaaat ggtatgctgt ttgaaaccga
     7981 aggtgaagaa ctgcgcaccg tggcaaccga cggccaccgt ctggcggtct gttcaatgcc
     8041 aattggtcaa tctttgccaa gccattcggt gatcgtaccg cgtaaaggcg tgattgaact
     8101 gatgcgtatg ctcgacggcg gcgacaatcc gctgcgcgtg cagattggca gcaacaatat
     8161 tcgcgcccac gttggcgact ttatcttcac ctccaaactg gtggatggtc gcttcccgga
     8221 ttaccgccgc gttctgccga agaatccgga caaacatctg gaagctggct gcgatctgct
     8281 caagcaggcg tttgcccgtg cggcaattct ctctaacgag aaattccgcg gcgtgcgcct
     8341 gtatgtcagc gaaaaccagc tgaaaatcac cgccaacaac ccggaacagg aagaagcgga
     8401 agagatcctc gacgttacct atagcggtgc ggagatggaa atcggcttca acgtcagcta
     8461 tgtgctggat gttctgaacg cgctgaaatg cgaaaacgtc cgcatgatgc tgaccgattc
     8521 ggtttccagc gtgcagattg aagatgccgc atcacagtcg gctgcctatg ttgtcatgcc
     8581 aatgagactg taatgtccct cacccgcttg ttgatccgcg atttccgcaa cattgaaacc
     8641 gcggatctcg ctttatctcc cggctttaac tttctggtag gtgccaacgg cagtggcaaa
     8701 accagcgtgc tggaagccat ctatacgctc ggccatggtc gggcgtttcg cagtttgcag
     8761 attggtcgcg tcattcgcca tgagcaggag gcgtttgttc tccacgggcg attacagggc
     8821 gaagagcgcg agacagcgat tggcttaacc aaagacaaac agggcgacag caaagtccgc
     8881 atcgacggta cagacgggca taaggtcgcg gaactggcgc acctgatgcc aatgcagttg
     8941 ataacgccag aagggtttac tttactcaac ggcggcccca aatacagaag agcattcctc
     9001 gactggggat gctttcacaa cgaacccgga tttttcaccg cctggagcaa tctcaagcga
     9061 ttgctcaagc agcgcaatgc ggcgctgcgc caggtgacac gttacgaaca gctacgcccg
     9121 tgggataaag aactgatccc gctggcggag caaatcagca cctggcgcgc ggagtatagc
     9181 gccggtatcg cggctgatat ggccgatact tgtaagcaat ttctccctga gttttctctg
     9241 actttctctt tccagcgcgg ctgggagaaa gagacagaat atgctgaggt gctggaacgt
     9301 aattttgaac gcgatcgcca gctaacctac accgcgcatg gcccgcataa agcggactta
     9361 cgcattcgcg ccgacggtgc gccggtggaa gataccttat cgcgtgggca gcttaagctg
     9421 ttgatgtgcg ccttacgtct ggcgcaagga gagttcctca cccgtgaaag cgggcggcgg
     9481 tgtctctacc tgatagatga ttttgcctct gagcttgatg atgagcgtcg cgggctgctt
     9541 gccagccgct taaaagcgac gcaatcacag gtctttgtca gcgcgatcag tgctgaacac
     9601 gttatagaca tgtcggacga aaattcgaag atgtttaccg tggaaaaggg taaaataacg
     9661 gattaaccca agtataaatg agcgagaaac gttgatgtcg aattcttatg actcctccag
     9721 tatcaaagtc ctgaaagggc tggatgcggt gcgtaagcgc ccgggtatgt atatcggcga
     9781 cacggatgac ggcaccggtc tgcaccacat ggtattcgag gtggtagata acgctatcga
     9841 cgaagcgctc gcgggtcact gtaaagaaat tatcgtcacc attcacgccg acaactctgt
     9901 ctctgtacag gatgacgggc gcggcattcc gaccggtatt cacccggaag agggcgtatc
     9961 ggcggcggaa gtgatcatga ccgttctgca tgcaggcggt aaatttgacg ataactccta
    10021 taaagtgtcc ggcggtctgc acggcgttgg tgtttcggta gtaaacgccc tgtcgcaaaa
    10081 actggagctg gttatccagc gcgagggtaa aattcaccgt cagatctacg aacacggtgt
    10141 accgcaggcc ccgctggcgg ttaccggcga gactgaaaaa accggcacca tggtgcgttt
    10201 ctggcctagc ctcgaaacct tcaccaatgt gaccgagttc gaatatgaaa ttctggcgaa
    10261 acgtctgcgt gagttgtcgt tcctcaactc cggcgtttcc attcgtctgc gcgacaagcg
    10321 tgacggcaaa gaagaccact tccactatga aggcggcatc aaggcgttcg ttgaatatct
    10381 gaacaagaac aaaacgccga tccacccgaa tatcttctac ttctccaccg aaaaagacgg
    10441 tattggcgtc gaagtggcgt tgcagtggaa cgatggcttc caggaaaaca tctactgctt
    10501 taccaacaac attccgcagc gtgacggtgg tactcacctg gcaggcttcc gtgcggcaat
    10561 gacccgtacc ctgaacgcct acatggacaa agaaggctac agcaaaaaag ccaaagttag
    10621 cgccaccggt gacgatgcgc gtgaaggcct gattgcggtc gtttccgtga aagtgccgga
    10681 cccgaaattc tcctcccaga ccaaagacaa actggtttct tctgaggtga aatcagcggt
    10741 tgaacagcag atgaacgaac tgctggcaga atacctgctg gaaaacccaa ccgacgcgaa
    10801 aatcgtggtt ggcaaaatta tcgatgctgc ccgtgcccgt gaagcggcgc gtcgcgcgcg
    10861 tgaaatgacc cgccgtaaag gtgcgctcga cttagcgggc ctgccgggca aactggcaga
    10921 ctgccaggaa cgcgatccgg cgctttccga actgtacctg gtggaagggg actccgcggg
    10981 cggctctgcg aagcaggggc gtaaccgcaa gaaccaggcg attctgccgc tgaagggtaa
    11041 aatcctcaac gttgagaaag cgcgcttcga taagatgctc tcttctcagg aagtggcgac
    11101 gcttatcacc gcgcttggct gtggtatcgg tcgtgacgag tacaacccgg acaaactgcg
    11161 ttatcacagc atcatcatca tgaccgatgc ggacgtcgac ggctcgcaca ttcgtacgct
    11221 gctgttgacc ttcttctatc gtcagatgcc ggaaatcgtt gaacgcggtc acgtctacat
    11281 cgctcagccg ccgctgtaca aagtgaagaa aggcaagcag gaacagtaca ttaaagacga
    11341 cgaagcgatg gatcagtacc agatctctat cgcgctggat ggcgcaacgc tgcacaccaa
    11401 cgccagcgca ccggcattgg ctggcgaagc gttagagaaa ttggtgtctg agtacaacgc
    11461 gacgcagaaa atgatcaacc gcatggagcg tcgttatccg aaagcaatgc tgaaagagct
    11521 tatctatcag ccgacgctga cggaagccga cctctctgat gagcagaccg ttacccgctg
    11581 ggtgaacgcg ctggtcagcg aactgaacga caaagaacag cacggcagcc agtggaagtt
    11641 tgatgtccac accaatgccg aacaaaacct gttcgagccg attgttcgcg tgcgtaccca
    11701 cggtgtggat actgactatc cgctggatca cgaatttatc actggtggcg aatatcgtcg
    11761 tatctgcacg ctgggtgaga aactgcgtgg cttgctggaa gaagatgcat ttatcgaacg
    11821 tggcgagcgt cgtcagccgg tagccagctt cgagcaggcg ctggactggc tggtgaaaga
    11881 gtctcgtcgc ggtctctcca tccagcgtta taaaggtctg ggcgagatga acccggaaca
    11941 gctgtgggaa accaccatgg acccggaaag tcgtcgtatg ctgcgcgtta ccgttaagga
    12001 tgcgattgcc gctgaccagt tgttcaccac gctgatgggc gacgccgtag aaccgcgtcg
    12061 tgcgtttatt gaagagaacg ccctgaaagc ggcgaatatt gatatttaat ggcgttaacc
    12121 atgcgagcgt gcctgatgcg ctacgcttat tagtcctacg agactcctgc aatatattga
    12181 ttttgcatga ttttgtaggc cggataaggc gttcacgccg catccggcaa gaagctataa
    12241 gaaaagggcg gagatcatct ccgccctttt tatttctgca atccgcaatt atcatcccgc
    12301 aaatctatac tttttgggtc aacacgtaac gctttacagg tgattatcat ggggcttttt
    12361 gatgaagttg tcggtgcctt tctgaaaggc gatgcgggga aatatcaggc tattttaagt
    12421 tgggttgagg agcagggcgg cattcaggtg ctgctggaaa aactgcaaag tggcggctta
    12481 ggggccattc tctcaacctg gctgagtaat caacagggca atcaatcggt tagtggcgag
    12541 caactggaat cggcgctcgg cacaaatgcg gtgtccgatc tcgggcaaaa acttggcgtg
    12601 gataccagta cagcttccag tttactggca gagcaattgc cgaagattat tgatgcgctc
    12661 tcaccgcaag gtgaagtgtc accacaagcc aataacgatc tgctttccgc aggtatggaa
    12721 ctgctgaaag ggaaactctt ccgctaagca aatggggagc atgcaacccg cgttgcatcc
    12781 cccttttccc tcgggtgttg tactgatttt tgagcggaat cgcgttagca tgggtcagga
    12841 accaatctac ctggggaact catggctatc aaactcattg ctatcgatat ggatggcacc
    12901 cttctgctgc ccgatcacac catttcaccc gccgttaaaa atgcgattgc cgcagctcgc
    12961 gcccgtggcg tgaatgtcgt gctaacgacg ggtcgccctt atgcaggcgt tcacaactac
    13021 ctgaaagagc tgcatatgga acagccgggc gactactgca ttacttataa cggcgcgttg
    13081 gtacagaagg ccgccgatgg tagcaccgtg gcgcaaaccg ctctcagcta tgacgactac
    13141 cgtttcctgg aaaaactctc tcgcgaagtc ggttctcatt tccacgccct ggaccgcacc
    13201 acgctgtata cagcaaatcg cgatatcagc tactacacgg tgcatgaatc cttcgttgcc
    13261 accattccgc tggtgttctg cgaagcggag aaaatggacc ccaataccca gttcctgaaa
    13321 gtgatgatga ttgatgaacc cgccatcctc gaccaggcta ttgcgcgtat tccgcaggaa
    13381 gtgaaagaga aatataccgt gctgaaaagt gcgccgtact tcctcgaaat cctcgataaa
    13441 cgcgttaaca agggtacggg tgtgaaatca ctggccgacg tgttaggtat taaaccggaa
    13501 gaaatcatgg cgattggcga tcaggaaaac gacatcgcaa tgattgaata tgcaggcgtc
    13561 ggtgtggcga tggataacgc tattccttcg gtgaaagaag tggcgaactt tgtcaccaaa
    13621 tccaaccttg aagatggcgt ggcgtttgct attgagaagt atgtgctgaa ttaatcagtg
    13681 gacgggcaaa cagcataaac gcgtttgccc gcttactgat tattggttta ctgcatatac
    13741 aaccagaaat tgcagcggtg cagtaatgat gtccagcgta acagtcactg gcagtaatgc
    13801 tgcggaaaca ccatccggaa tgcctctggg tgaatagtat tcataaaatc gcactttaaa
    13861 tggatggtag aacgccatca cctttgagta atccatgttt ttatttttct catggattgt
    13921 gccttttagg ttttccagtg acctggtaca gtgttgaaca ccacaggcaa aaccataatg
    13981 cgttgccagt gccttttctt cttcgttatt ccagtagtag taaagcgata ttgtgccggt
    14041 aaatttcttt ttccccgcat ttaaaacaaa tcttgcgtca tcggcaacct gaatattgtg
    14101 ccggttaagt gctggatcat tgagcatctt aacgatgtca tcggcaccat cagtgataag
    14161 gtaatccagc gattccccta caaaaacata gccttttgtt ccattactat cttttgcctg
    14221 cgatagccca gtgatggtgt ctgactgcga ttgatacttt cctgaactac cctttgcggt
    14281 atgggaaacc agagcatacg agatacagcc tgaaagcatc agcgccagtg ggaataaacc
    14341 ggcagcctta aaaaatccct taaaattcaa cttcatgatt aatcctttaa acgagtagct
    14401 aagccgttga aagcatactc ttctttccct cctgtttagc gcctgatatc ccttttcaga
    14461 tttctgcccg acgcatgtca tttttttatg cattgttctt tttgtgatct aaattgtagt
    14521 acaacaatat aagtttgtac tacattacgc acacggcaaa cgcgaacgtc atcacgctgg
    14581 tactacaaag ttgccgcgtt atgcatcgat cggggtaaag tagagaagaa catacagagc
    14641 acaaggactc tccatgactc tcaataaaac cgatcgcatt gtcattacgc tgggtaaaca
    14701 gatcgttcac ggcaaatacg tacctggctc gccacttccg gctgaggcgg agctctgtga
    14761 agagtttgca acctcgcgca acatcatccg tgaggtgttc cgttcgctga tggcgaagcg
    14821 gctgattgaa atgaaacgtt atcgcggggc gtttgtggca ccgcgtaacc agtggaatta
    14881 cctcgacact gacgtactgc aatgggtgtt ggaaaacgac tacgacccac ggcttatcag
    14941 tgccatgagc gaagtgcgaa atctggtgga accggcgatt gcccgttggg cagcagagcg
    15001 cgcgacctcc agcgatctgg cgcagattga atcggcgctg aacgagatga ttgccaacaa
    15061 tcaggaccgc gaagcgttta acgaagcgga tattcgctac cacgaggcgg tgctgcagtc
    15121 ggtgcataac ccggtgttac agcaacttag cattgcgatc agttcgctgc agcgggcggt
    15181 ttttgaacga acctggatgg gcgatgaggc caacatgccg caaacgctcc aggaacataa
    15241 ggcgctgttc gatgcgatac ggcatcagga cggcgatgcg gcagagcagg cggcgcttac
    15301 catgatcgcc agctcgacac gaaggttaaa ggaaatcaca tgacagctcg ctacatcgca
    15361 attgactggg gatcgaccaa tcttcgcgcc tggctttatc agggcgacca ctgcctggag
    15421 agcaggcaat cagaagcagg cgtcacgcgc ctgaacggaa aatctccggc tgcggcgtta
    15481 gcagaagtca cgaccgactg gcgtgaagag aatacgccag tggtaatggc aggaatggtc
    15541 ggcagcaatg tcggctggaa agttgctccg tatttatctg ttcctgcccg tttttcgtct
    15601 attggcgaac aattaacgtc tgttggcgac aatatctgga ttattcccgg attatgcgtc
    15661 tctcatgacg ataaccacaa tgtgatgcgc ggcgaagaaa cacaattgat cggcgcgcga
    15721 actctggctc cttcctctct ttatgttatg cccggtacgc attgcaaatg ggtgcaggcc
    15781 gatagccagc aaatcaacga ttttcgcacc gtgatgaccg gtgaactaca tcatttactg
    15841 ttaaatcact cattgattgg cgcaggtttg ccgccgcagg aaaactctgc cgatgccttc
    15901 gcggctggcc ttgagcgcgg ccttaatgcg cccgccatat tgccgcagct ttttgaagtt
    15961 cgcgcctcgc atgtgctggg aacacttccc cgcgaacagg tcagcgaatt tctctctggt
    16021 ttgttgattg gcgcagaggt tgccagtatg cgcgactatg tgacccatca acacgccatc
    16081 acccttgtcg ccggaacatc gctgaccgcg cgctaccagc aagcctttca ggcgatgggt
    16141 tgcgacgtga cggcggtggc gggcgacacg gcatttcagg ctggtataag gagcatcgct
    16201 catgcagtgg caaactaaac ttccgctgat cgccattttg cgcggcatta agcccgacga
    16261 ggcgctggcg catgttggcg cggtgattga cgccgggttc gacgcggttg aaatcccgct
    16321 gaattcccca caatgggagc aaagcattcc cgccatcgtt gatgcgtatg gcgacaaggc
    16381 gttgattggc gcaggtacgg tactgaaacc tgaacaggtc gatgcgctcg ccaggatggg
    16441 ctgtcagctc atcgttacgc ccaatatcca tagtgaagtg atccgccgtg cggtgggcta
    16501 cggcatgacc gtctgccccg gctgcgcgac ggcgaccgaa gcctttaccg cgctcgaagc
    16561 gggcgcgcag gcgctgaaaa tatttccgtc atcggctttt ggtccgcaat acatcaaagc
    16621 gttaaaagcg gtattgccat cggacatcgc agtctttgcc gttggcggcg tgacgccaga
    16681 aaacctggcg cagtggatag acgcaggttg tgctggggcg ggcttaggca gcgatctcta
    16741 tcgcgccggg cagtccgtag aacgcaccgc gcagcaggca gcagcatttg ttaaggcgta
    16801 tcgagaggca gtgcaatgaa aatcaccaaa attaccacgt atcgtttacc tccccgctgg
    16861 atgttcctga aaattgaaac cgatgaaggc gtggtcggtt ggggcgagcc cgtgattgaa
    16921 ggccgcgccc gtacggtgga agcggcagtt cacgagctgg gtgactattt gattggtcag
    16981 gatccttcgc gcatcaatga cttatggcaa gtgatgtatc gcgccggatt ttatcgtggc
    17041 ggtccaatcc tgatgagcgc cattgccggg atcgaccagg cgttatggga tatcaaaggc
    17101 aaagtgctga atgcgccggt ctggcaactg atgggcggcc tggttcgcga caaaattaaa
    17161 gcctacagtt gggtcggcgg cgatcgtccg gcggatgtta tcgacggcat taaaaccctg
    17221 cgcgaaatcg gcttcgatac cttcaaactg aacggttgtg aagaactggg gctaattgat
    17281 aactcccgcg cggtagatgc ggcagtcaac accgtggcac aaattcgtga agcttttggc
    17341 aatcagattg agtttggtct tgatttccac ggtcgcgtca gcgcgccaat ggcgaaagtg
    17401 ctgattaaag aactggagcc gtatcgcccg ctgtttattg aagagccggt gctggcggaa
    17461 caagccgaat actacccgaa actggcggca caaacgcata ttccactggc ggcgggtgag
    17521 cgcatgttct cacgtttcga ttttaaacgc gtgctggagg caggcggtat ttcgattctg
    17581 caaccggatc tctcccatgc aggcggtatt accgaatgct acaaaattgc tggaatggca
    17641 gaagcctatg atgtgaccct tgcgccgcac tgtccgctcg gaccgattgc actggcggct
    17701 tgcctgcata tcgactttgt ttcctataac gcggtacttc aggaacaaag tatgggcatt
    17761 cattacaaca aaggcgcgga gttactcgac tttgtgaaaa acaaagaaga cttcagtatg
    17821 gttggcggct tctttaaacc gttaacgaaa ccgggcttag gtgtggaaat cgacgaagct
    17881 aaagttattg agttcagtaa aaatgccccg gactggcgta atccgctctg gcgtcatgaa
    17941 gataacagcg tagcagagtg gtaattcctg ccacgtaagc ccctcatcgg gcactaaaac
    18001 agcaatacaa aaatataact ataaccctct gtaaattaca gggcatggtg agcggcttcg
    18061 ctatgcccaa aatctggaga aagctcgcta tggatattcc cgttaatgca gcaaagccgg
    18121 ggcgtcggcg ttatctgacg ctggtgatga tctttattac ggtggtcatt tgttatgttg
    18181 accgcgctaa cctggccgtg gcttccgccc atattcagga agagttcggc attacgaaag
    18241 cggaaatggg ctatgtattt tcggccttcg cctggcttta tacgctatgc cagatccccg
    18301 gcggttggtt tttagatcgc gtaggttctc gcgtgactta ttttattgcg atatttggtt
    18361 ggtcagtggc gactttattc cagggctttg ccacgggatt aatgtcatta attggtctgc
    18421 gtgcgataac cggtattttc gaagcgcccg ctttcccgac caataaccgg atggtgacca
    18481 gctggttccc ggaacatgaa cgcgcttccg ccgttggttt ttatacgtct ggtcagtttg
    18541 tcggtctggc gtttttgact ccgctgctga tctggattca ggagatgttg agctggcact
    18601 gggtgttcat tgtcactggt ggtatcggca ttatctggtc gctgatttgg tttaaggttt
    18661 atcagccgcc gcgcctgacc aaaggtatca gcaaagctga actggattac attcgtgatg
    18721 gcggcggtct ggtggatggt gatgcgccgg tgaagaaaga ggcgcgtcag ccgttaacag
    18781 ccaaagactg gaaactggtg ttccatcgta aactgatcgg cgtttatctt gggcaatttg
    18841 cggtggcttc tacactgtgg tttttcttaa cctggttccc gaactattta acccaggaaa
    18901 aaggaatcac ggcgctgaaa gcaggcttta tgaccacggt gccattcctc gcggcgtttg
    18961 tcggcgtcct gctctctggc tgggtcgcgg atctgctggt acgtaagggc ttttcactgg
    19021 gctttgcgcg taaaacgccg attatctgcg gcttgctgat ctccacctgc attatgggcg
    19081 ctaactacac taacgatccg atgatgatta tgtgcctgat ggcgctggca ttcttcggca
    19141 acggttttgc ttcgattacc tggtcgctgg tctcttctct ggcaccgatg cgcctgattg
    19201 gtttaaccgg cggcgtgttt aacttcgccg gtggtctggg cggcatcacc gttccgctgg
    19261 tggtggggta cctggcgcag ggttacggtt tcgcacctgc actggtttat atctccgccg
    19321 tcgcgttgat tggcgcgctc tcttacatcc tgctggtggg cgatgtgaag cgcgttggat
    19381 aatctttcaa ctgtggaata tgcgccacac cactgcgcat aatccacttt cttaaagccg
    19441 gtgcggtcag gcatcggctt tttactatct tgccgaagag ttttaaacgc agtttgcggg
    19501 tggcgcgcca gtaggcggcg ttgctcttct ccggctgctt cagtaacacc gcacgcagaa
    19561 tctctgcgct atccagcgca tagctaatcc cctccagcga gctggcgctg ataaatcctg
    19621 ccgcttcgcc aatcagaaag gcgttgtcct taccgcagac aaaatcctgc cagcgcgagg
    19681 gaaacagcac cgtgcatttt tcgcttttca ccgccttacc aaactggaac tgaaaggcgc
    19741 tcattttctc tttcagcgtc gtgaaacgcg tctgaccgtc tttcattgga taagcaccgc
    19801 caaagataaa ataaccgtct ttgctgatac tccatgaata acagtcagtt atttcattat
    19861 caaagatgca ggagtagaac ggtaccggat gtttctctgc aaaccactgc tggatagcga
    19921 catatttacg gatttgatga tccgggtaga gatgtcgccg caccatcgag ttggcaccat
    19981 cggcaccgac cagatagcgg gcggaaatat gctgctccca accgtctgca cgaaaaatga
    20041 catgccattt atcatcctca cgccagattt tccggcacag gctatcgtgg taaacctcaa
    20101 cgctggcggg gatcagtgat ttcatccaca agtcgaaagc gtggcgatta atattgatat
    20161 agcttcgctg gtagttacgt gtgagcgatg cggcgacgtc gacggttttg acgctgaaaa
    20221 tctgcggatt ggcgatcaca tcgacaggga gcgtcagtcc atcgcgaata aaagaacgct
    20281 gcgcgtccgg tgccagcaga ccgccacaag gtttgctgaa accttcagta ccacactggt
    20341 gctttttatc caacgcgatc acctgcattt tgccggctaa ctttcgtgcc aacgccgacc
    20401 ctgccgggcc gaggccaata atcgccacgt cgaaatgttc catcttttca ctcacattca
    20461 tcacgatggc aaaagactat tgtctgaaag tgaagtcctt gtgaagtatg atggcgggac
    20521 ccgttttgta tcaggaccgc gccatgaaac agataacctt tgctccccgt aatcacctgc
    20581 tcaccaatac caatacttgg acgcccgaca gccagtggct ggtatttgac gtgcgtcctt
    20641 ctggcgcgtc gtttaccggc gagaccattg agcgtgtgaa tatccatacc ggcgaggtcg
    20701 aggttatcta tcgcgcgtca cagggcgcac acgtcggcgt ggtgaccgtt catccaaagt
    20761 cagagaaata tgtctttatt cacggcccgg aaaatcctga tgaaacatgg tattacgatt
    20821 tccatcaccg tcgcggcgtg attgctgaaa gcggcaaggt gagcaatctc gatgcaatgg
    20881 atattactgc accgtacacc ccaggagcgc tgcgcggcgg cagccatgtg catgtcttta
    20941 gcccgaacgg tgaaagggtg agctttacct ataacgacca tgtaatgcat gaactcgatc
    21001 cggcgctgga tttgcgaaac gtcggtgttg ctgcgccgtt tggcccggtc aacgtacaaa
    21061 agcagcatcc gcgtgaatac agcggtagcc actggtgcgt gctggtgagt aaaaccacgc
    21121 ccacgccgca gcctggcagt gatgaaatca atcgtgctta tgaagaagga tgggtaggaa
    21181 atcacgcgct ggcgtttatt ggcgacacac tttcgccaaa gggcgagaaa gtgccggagc
    21241 tgtttatcgt tgagttaccg cacgatgaag ctggctggaa agcagctggt gatgcgccgt
    21301 taagcggaac ggaaaccacc ctgcccgcac caccgcgtgg cgtcgtgcag cgacgtttaa
    21361 cctttaccca ccatcgggct tatccggggt tagtcaacgt cccgcgccac tgggtgcgct
    21421 gtaatccgca gggtacgcaa atcgcgtttt taatgcgtga tgataacggc attgtgcaac
    21481 tgtggcttat ctcgccacag ggcggcgagc cgcgccagtt aacccataac aaaacggata
    21541 ttcagtctgc atttaactgg catccgtcag gagaatggtt gggctttgtg ctggataatc
    21601 gaattgcttg tgcccatgcg caaagcggcg aggttgagta tttaaccgaa aaccacgcca
    21661 atccaccttc tgcggacgcc gtggtcttct cgccggatgg tcaatggctg gcgtggatgg
    21721 aaggtggcca gctgtggatc accgaaactg atcgctaaat cagttatcag gcattctggc
    21781 cggtggaata accgcattgg tggcttgcgc gttggcttcg cttttttcaa cgcgtgagcg
    21841 caccgagctg tctttgcgga acacatccca cggcagtaaa agcgtgtcca tcacagcggt
    21901 aaacggcata tcgagaatcg ccagcgactt agtgccccag ttggtttcat cgtcgccaat
    21961 catggttgcg ctggcgcggg tgccgggata cgttccttct ttccctccgg tatgggacat
    22021 cacgctggag catcctccga gtaatgtcat tccgctgcat atcatgaacg ctaacaacac
    22081 atttcttatc ataataaatt catctgttga tcgtgggtgt tggcctgatg agttatagcg
    22141 atcccttgct gaaaataaca tcatcattac gtcgcactgt ggcggctatc gcactttaac
    22201 gtttcgtgct gccccctcag tctatgcaat agaccataaa ctgcaaaaaa aagtccgctg
    22261 ataaggcttg aaaagttcat ttccagaccc atttttacat cgtagccgat gaggacgcgc
    22321 ctgatgggtg ttctggctac ctgacctgtc cattgtggaa ggtcttacat tctcgctgat
    22381 ttcaggagct attgattatg cgtaactttg atttatcccc gctttaccgt tctgctattg
    22441 gatttgaccg tttgtttaac cacttagaaa acacccagag ccagagtaat ggcggctacc
    22501 ctccgtataa cgttgaactg gtagacgaaa accattaccg cattgctatc gctgtggctg
    22561 gttttgctga gagcgaactg gaaattaccg cccaggataa tctgctggtg gtgaaaggtg
    22621 ctcacgccga cgaacaaaaa gagcgcacct atctgtacca gggcatcgct gaacgcaact
    22681 ttgaacgcaa attccagtta gctgagaaca ttcatgttcg tggcgctaac ctggtaaatg
    22741 gtttgctgta tatcgatctc gaacgcgtga ttccggaagc gaaaaaaccg cgccgtatcg
    22801 aaatcaacta attccttaag gccgcctggc gcggcctgac atctccatgc tcgccgtcag
    22861 ggagcatatg cgaatcttcg gatttgcagg tacttactcg cttcttagaa ggagaaatga
    22921 ctatgcgtaa cttcgattta tccccactga tgcgtcaatg gatcggtttt gacaaactgg
    22981 ccaacgcact gcaaaacgcc ggtgaaagcc agagcttccc gccgtacaac attgagaaaa
    23041 gcgacgataa ccactaccgc attacccttg cgctggcagg tttccgtcag gaagatttag
    23101 agattcaact ggaaggtacg cgcctgagcg taaaaggcac gccggagcag ccaaaagaag
    23161 agaaaaaatg gctgcatcaa gggcttatga atcagccatt tagcctgagc tttacgctgg
    23221 ctgaaaatat ggaagtctct ggcgcaacct tcgtaaacgg tttactgcat attgatttaa
    23281 ttcgtaatga gcctgaaccc atcgcagcgc agcgtatcgc tatcagcgaa cgtcccgcgt
    23341 taaatagcta actagctatt ctctttgccc cgccattctg gtggggcttt ttttgtcttt
    23401 cttcttcaac aatgcgttaa atttcgcctg tttaagatat ttcggcacgt tctttcccgt
    23461 gataatgtgc gctataaggc aaatctgctt caccgattca gccataatcc gtagcaatta
    23521 aaaatgttat ctcaagagaa atggacgatg agtgatatag cattaacggt cagtattctg
    23581 gctttggtgg cagtcgtcgg tttgtttatc ggcaatgtca aatttcgcgg cataggatta
    23641 ggtattggcg gcgtgctgtt tggtgggatc atcgtcggcc attttgtttc tcaggcgggg
    23701 atgacattaa gtagcgatat gctgcatgtt attcaggaat ttggcctgat cctgttcgtt
    23761 tatactatcg ggattcaggt agggccgggc ttctttgcct cattgcgcgt ctccggatta
    23821 cgcctcaacc tgtttgctgt tctgatcgtc atcatcggtg gtctggttac cgccatcctg
    23881 cataaactgt ttgatattcc actgccggta gtgctgggga ttttctccgg tgcggttacc
    23941 aatacgccag cgctgggggc agggcagcag attttgcgcg acctgggtac accaatggaa
    24001 atggtcgatc agatggggat gagttacgcg atggcgtatc cattcggcat ttgcgggatt
    24061 ttgttcacca tgtggatgtt gcgggttatt ttccgcgtca atgtcgagac agaagctcag
    24121 cagcacgagt cttcacgcac caatggcggc gcgctgatca agactatcaa tattcgcgtt
    24181 gagaacccta acctgcatga tttagccatt aaagatgtac cgattctcaa cggcgacaaa
    24241 attatctgct cgcgtctgaa acgcgaagaa accctaaaag ttccttcgcc agataccatt
    24301 attcaactgg gcgatttgct gcatctggtg ggtcagccag cggatttaca taatgcgcaa
    24361 ctggtgattg gtcaggaggt cgatacttcg ctgtccacga aaggcactga tttgcgcgtc
    24421 gagcgtgtgg tggtcaccaa tgaaaacgtg ctcggaaaac gtattcgcga cctgcacttt
    24481 aaagaacgct atgacgttgt tatctcgcgc ctgaaccgtg ccggggtcga actggtcgcc
    24541 agtggcgata tcagcctgca gttcggcgat atcctcaacc tggtggggcg tccgtccgca
    24601 attgatgccg ttgccaatgt gctggggaat gcgcagcaaa aactgcaaca ggttcagatg
    24661 ctgccagtgt ttattggcat cgggcttggc gtattgttag gttctattcc cgtctttgtg
    24721 ccaggattcc cggccgcgtt gaaactgggg ctggcgggcg gtccgctgat tatggcgttg
    24781 atcctcgggc gtatcggcag tatcggcaag ctgtactggt ttatgccgcc aagtgccaac
    24841 ctcgcgctgc gggagctggg gatcgtgctg ttcctctcgg tcgttggtct gaaatctggt
    24901 ggggattttg taaataccct ggtcaatggc gaagggctaa gctggattgg ttatggtgcc
    24961 ctgatcaccg ccgttccgct gattactgtt ggcattctgg cgcggatgtt agccaaaatg
    25021 aattacctga ccatgtgcgg gatgctggca ggttccatga ccgatcctcc ggcgctggcg
    25081 tttgctaata atcttcatcc aaccagcggt gcggcggcgc tctcttacgc cactgtctat
    25141 ccgttagtga tgttcctgcg cattatcacc ccccaattac tggcggtgct cttctggagt
    25201 atcggttaac tcttttccgg gtggagtcgc cgtaaatggt actccacctg gtattcgctg
    25261 gcatttctga acatcactga ataattcaaa aactccccgc tctcgctata tgacaacgag
    25321 gtgatccgca gcagtggcgt gtgttccgct accttcattt gccgcgccag cagtctatcg
    25381 gcgagcactg gcgtcaggct ttcataatta ccgccaatca aaatcccgca ctcttgttca
    25441 atatattcaa acttcgaccc ttccagatgt tgcagcgaaa gattacggaa cagttttacc
    25501 ggcatatagc tgtcttccag catcagcggt ttcccttcca caaaacgaac gcgacgggag
    25561 aagtagatct gctcgttgat ttgaatccgt aactggctgg caatggccgg aggcgcaggc
    25621 atgatttcaa aaatcagcac ctggctggtg accgtttttc cctgccgttt taacacctcc
    25681 accagcccgg tcaggctggc ggtttgatgc agtacatctt tgcgcaccag gtaagtgccg
    25741 ctgccgtggc ggcggaccac caatccccac gctaccagca ggtcaatcgc tttacggatg
    25801 gtcatccggg atactgcaaa ttcttccgcc aacttttttt caccgggaag aaggctgttt
    25861 agcgtgaaat ccgcggaatt aagtcgaatt cttaaccgct ccgcaatgct tttgtagatc
    25921 atctctagat ctcttttttg cctgcaagcc cgggtaatgc gcccacaagt tctgcttaat
    25981 cgattgaaaa aacatctttt ttttaaagat gtgttcgatg ctgtgtacca gtgctcacag
    26041 atgtctactt tttcgcgaaa acgtagatct ctaccgccca acgaaaagca tgaaagcgat
    26101 cacgaatccc attgggtcgt catgttctgc cgatcgcatc ttcctatcct cgctccaggc
    26161 ctgccgcata accaatcagg cttcctactt acagaattga gaaaagagga tgtggaaatg
    26221 ctcagtcaaa ttcaacgctt tggcggcgcg atgttcacgc cagtgctgct gtttcccttc
    26281 gccgggattg tggtgggtct tgccatcttg ctgcaaaacc cgatgtttgt cggggaatca
    26341 ctgaccgatc cgaacagttt attcgcgcaa atcgtacaca ttattgaaga gggcggttgg
    26401 acggtattcc gtaatatgcc gctgattttt gctgtcggtt tacccattgg ccttgctaag
    26461 caagcgcagg ggcgtgcttg tctggcggtg atggtgagtt tcctgacctg gaactatttc
    26521 atcaacgcga tgggaatgac ctggggaagc tacttcggcg tcgatttcac tcaggacgcg
    26581 gtggcaggta gcggtctgac aatgatggcc gggattaaaa ccctcgatac cagcattatc
    26641 ggcgcaatta tcatttccgg cattgtgacg gcgctgcata accgtctgtt cgataaaaaa
    26701 ctgccggttt ttctcggcat tttccagggg acgtcttatg tggtgattat cgccttcctg
    26761 gtgatgatcc cctgtgcctg gctgacattg ctcggctggc caaaagtaca aatggggatt
    26821 gaatctctgc aagcgttcct gcgttcggcg ggtgcgcttg gggtgtgggt ttacaccttc
    26881 ctcgaacgta ttctgatccc aaccggttta caccacttca tctacggaca gtttatcttt
    26941 ggtccggcag ctgttgaagg cggcattcag atgtactggg cgcagcatct gcaagagttc
    27001 agtctgagcg ccgagccgct gaaatcgttg ttcccggaag gcggttttgc cctgcacggt
    27061 aactcaaaaa tctttggtgc cgtgggcatt tctttagcga tgtacttcac tgccgcaccg
    27121 gaaaatcggg taaaagtggc gggcttgctg attcccgcaa ccttaaccgc catgctggtg
    27181 ggaattaccg aaccgctgga atttaccttc ctgttcattt caccgttgct gtttgcggta
    27241 cacgctgtgc ttgcggcctc aatgtcgacc gtgatgtatc tctttggtgt ggtgggcaac
    27301 atgggcggag gtctgattga ccaggtttta ccgcaaaact ggatcccgat gttcagcaac
    27361 cacgcggata tgatgctgac ccaaatcgcc attgggttgt gctttaccct gctgtacttc
    27421 gtggttttcc gcacactgat tctgcagttc aacatgtgca cgccgggacg tgaagatgcg
    27481 gaagtgaaac tctactcaaa agccgaatac aaagcctcgc gaggccaaac caccgcggca
    27541 gagccaaaaa aagagctgga tcaggctgcc ggtatcctgc aagccctggg cggggtcggc
    27601 aatatctcca gcattaacaa ttgtgcgacg cgtctacgca ttgcactgca tgacatgtca
    27661 caaacgctgg atgacgaagt ctttaaaaag ctgggagcgc acggcgtctt ccgtagtggc
    27721 gatgccattc aggtgatcat tggtctgcat gtatcccagc tgcgtgaaca gctcgatagc
    27781 ttaattaatt ctcatcaatc agcagaaaat gttgccatta cggaggcagt ataatgacca
    27841 aattctcagt ggttgtcgca ggcggtggaa gcacctttac gccaggcatc gtgttgatgc
    27901 tcctggcgaa tcaggagcgt ttcccgcttc gtgcactgaa attttatgat aacgatggcg
    27961 cgcggcagga agtgattgcc gaagcctgta aagtcatcct taaagagaaa gcgccggaca
    28021 ttgcgtttag ttacaccacc gatcctgaag tggcgttcag cgacgttgat tttgtcatgg
    28081 cgcacatccg cgtcggcaag tacccgatgc gcgaactgga tgaaaaaatc ccactgcgcc
    28141 acggtgttgt tggtcaggaa acttgcggac ccggcggaat agcgtacggc atgcgttcca
    28201 ttggcggcgt cctggaactg gtggattata tggaaaaata ttcaccaaat gcctggatgc
    28261 tcaactactc caatccggca gccattgtcg cagaagccac gcgtcgtctg cgcccgaatg
    28321 cgaaaatcct caacatctgt gacatgccaa tcggtattga aagccggatg gcgcaaattg
    28381 ttgggctgca agatcgcaaa cagatgcgcg tgcgctacta cggcctgaac cactggtggt
    28441 cggcgatatc ccgcagtttc agaaagggtt aatgagtcag caagtggcgg tggaaaaact
    28501 ggtcgtggac gcctgggaac agcgttcata tcagcacctg tggcaggcga ttacgttgtc
    28561 gaaaacggta ccgagcgcct cggtcgccaa agctattctg gatgagttgc tggaggccaa
    28621 caaagcgtac tggccagagt tacgttaatt gtgtggactg gcatccgttg ggtgctggtc
    28681 cgtattcttt agcacggctg acggcgcata accaaattta tgtttaaagg ctttgctgaa
    28741 atgaaaggag tcgaagaagt tgagcgtatc agcgacttcg ccaaccgagt ttccttgttg
    28801 ctttaatagc gatagcgcca gatccagacg cgcatcgagg taatattctt tcggcgtctt
    28861 gccggtatag cgtaaaaaca gacgacgcaa ccaggcttcg ctacagggga tcgtggcagc
    28921 catatcagct acgctccagc gttgttgcag actggcatgt aaagtggcaa ttaatttttc
    28981 aatttgccgc cgttgtggat cttttttacc atctgcgtac ataagacaaa tccactgata
    29041 aataattttc gtcagaaaag cgactgccag attatttttt attgcttctg gtgaagttat
    29101 taactcagca acttcggtga gttcctgatt ataaatttcg ccattataaa taacgctttg
    29161 ctgaccaacg ggaatatcca tcatactggt gggggtaaat tccatccagt actgttccca
    29221 gactaaacct tcacagtgat aagagtgaat atccattggc tttaagaata taatacagtt
    29281 gccatgcagt gttatttgtt cgccattctt taaaagaatt ttcccacatc cttgtacggt
    29341 atacaccgca acccatgagt tggcgataag cggtttcttc ttgtcccgcg gccagatcac
    29401 ccggtaatgc tcgtcggcgt aggtatgcca gagcgagatc agagaaattc cgctggagat
    29461 cacattttcg ttaatcaata agggaatatt gtacggagtt tcgtttttta catctgaact
    29521 ttgcaatttt ccattcattt taatatccct ccggcgaatt atgctcacaa cctgaactta
    29581 acagaaaaca atccgttcag ggatacgacg tttgtatttt aagaatctga ctgcctgacc
    29641 cgacgcattt tacctctccc tatattcatg cgtccgggac ataaacataa ataagggcta
    29701 tgagatgaat tcgttacaaa tcttgagttt tgtcggtttt acgctgctgg tggcggtgat
    29761 cacctggtgg aaggtccgca aaatagatac cggatcgcaa caaggctatt ttcttgccgg
    29821 acgttcacta aaagcgccgg ttattgccgc ttcgttaatg ctaaccaacc tttccacgga
    29881 acaactggtc ggcctttccg ggcaggccta caaaagcggc atgtcggtga tgggctggga
    29941 agtgacttca gcggtgacgc tgatcttcct cgcgctaatc tttttaccgc gctatctgaa
    30001 gcgcggcatt gccaccatcc ccgattttct ggaggaacgt tatgataaaa cgacgcgtat
    30061 tatcatcgac ttctgcttcc tcattgccac cggcgtctgc tttctgccga ttgttctcta
    30121 ctccggcgcg ttggcgctca acagcctgtt tcacgtcggg gaatcgctac agatttctca
    30181 cggtgcggct atctggctac tggtaatttt gcttggtctg gcgggaattt tgtatgcggt
    30241 gatcggcgga ctgcgcgcaa tggcagtggc ggactccatc aacggtattg ggctggttat
    30301 cggcgggttg atggtgccgg tatttggcct gatcgcgatg ggcaagggca gctttatgca
    30361 gggcattgag caactcacca ccgttcacgc cgagaaatta aactcaatcg gtggcccgac
    30421 cgatcccttg ccgattggcg cggcatttac cggtttgatt ctggtgaaca ccttttactg
    30481 gtgtacaaat cagggcatcg tgcaacgcac gctggcgtca aaaagcctgg cggaagggca
    30541 aaagggggcg ctgttaacgg cggtgctgaa aatgctcgac ccgctggtac tggtgctgcc
    30601 agggttgatt gcgtttcatc tgtatcagga tttaccgaaa gccgacatgg cctacccgac
    30661 gctggtcaat aacgttctgc cagtgccaat ggtgggtttc ttcggcgcgg tgttatttgg
    30721 tgcggtgatc agtaccttca acggctttct gaatagcgcc agtacgttat tcagtatggg
    30781 tatttaccgt cgcatcatta accagaatgc cgagccgcag cagctggtca ccgtcgggcg
    30841 caaatttggt ttctttatcg ctatcgtttc ggtgctggtc gcgccgtgga tcgccaacgc
    30901 gccgcagggg ctgtatagct ggatgaaaca gctcaacggc atttacaacg tgccgctggt
    30961 taccatcatc attatgggct ttttcttccc gcgcatcccg gcgctggcgg caaaagtggc
    31021 gatggggatt ggcataatca gctacatcac catcaactat ctggtgaagt tcgacttcca
    31081 tttcctctat gtgctggcct gtacgttctg catcaacgtg gtcgtgatgc tggtgatcgg
    31141 ttttatcaaa ccgcgcgcca cgccgttcac cttcaaagat gcgtttgcgg tggacatgaa
    31201 accgtggaga aacgtcaaga tcgcgtcaat tggcatcctg ttcgcgatga ttggcgtcta
    31261 tgccgggctg gctgaattcg gcggctacgg tacgcgctgg ttagcgatga tcagttattt
    31321 cattgccgcc gtagtgattg tctacctgat ttttgacagc tggcggcatc gtcacgaccc
    31381 agccgtaacc tttactcccg acgcgaagga tagcctatga aacgccccaa ttttctgttc
    31441 atcatgaccg atacccaggc caccaatatg gtcggttgct atagcggtaa accgctgaat
    31501 acgcaaaata ttgatagtct ggcggcggaa ggtattcgct ttaattccgc ctacacctgt
    31561 tcaccggttt gtacgcctgc acgcgccgga ctatttaccg gtatctacgc taaccagtcc
    31621 ggcccgtgga ccaacaacgt cgcgccaggc aaaaacatct ccactatggg gcgctacttt
    31681 aaggatgccg gctatcacac ctgttacatc ggcaaatggc atctcgacgg tcatgactat
    31741 ttcggcactg gcgagtgtcc gccggagtgg gacgctgatt actggttcga tggggcgaac
    31801 taccttagcg aactgacgga aaaagagatc agcctgtggc gcaatggcct aaacagcgtt
    31861 gaggatttac aggcgaacca tatcgacgaa accttcacct gggcgcaccg catcagcaat
    31921 cgggcggtag attttctgca acagcccgcg cgcgccgagg aacccttcct gatggtggtt
    31981 tcgtatgatg agccgcatca cccgttcacc tgtccggtgg agtatttaga gaaatacgct
    32041 gatttttact acgatctggg cgagaaagct caggatgacc tggcgaacaa accggaacat
    32101 caccgcttat gggcgcaggc gatgccatcg ccagtcggtg atgacgggct ttatcaccat
    32161 ccgctctatt ttgcctgtaa tgactttgtt gatgaccaaa tcggacgggt catcaatgcc
    32221 ttaacgccag agcaacgtga aaatacgtgg gtcatttata cttccgatca cggcgaaatg
    32281 atgggcgcac ataagctgat cagtaaagga gcggcgatgt atgacgacat cacccgtatt
    32341 ccgctgatca tccgttcgcc gcaaggggag cggcgacagg tcgatacgcc agtcagtcat
    32401 atcgatttac tgccgacaat gatggcgctg gcagatattg aaaaaccaga gattctgccg
    32461 ggggaaaata tccttgccgt gaaagagcca cgcggtgtaa tggtggaatt taaccgctac
    32521 gagattgagc atgacagctt tggcggtttt attccggtgc gttgctgggt gacggatgac
    32581 tttaaactgg tactcaacct cttcaccagt gatgaacttt acgatcgccg taatgaccca
    32641 aatgaaatgc ataacctgat cgatgatatc cgttttgcag acgttcgcag caaaatgcat
    32701 gacgccttat tggattacat ggacaaaatt cgcgatccgt tccgcagtta ccaatggagc
    32761 ctgcgtccgt ggcgtaaaga tgcactgccg cgctggatgg gggcatttcg tccacgccca
    32821 caagatggct attcgccggt ggtacgtgac tatgacaccg gcctaccgac gcaaggagtg
    32881 aaagtggaag agaaaaaaca gaagttctga cgataatgcc cgctgatgaa cacccggcgg
    32941 gcattattca ggctgcttaa accagtgaac cgccagtcag gaaggaaaag ataaggaata
    33001 tcagcaaaat gctgcacacg atgccgatac caaaaagcaa ggtatggaaa gccagcgtcc
    33061 cgccgccaaa aaccagcgcg ccgcgaatgc cccagcgatt ttccttacgc ataccgccaa
    33121 taaatgcgca aagtagcgca gcaatggcca gtgcgatacc gctttgatcg aggattttat
    33181 cgacatctac gtcctctttt ttctccacgg tagtaatttt ttcgcctttt attccggcga
    33241 gaagacctct tttaatttca gccgttttgt ccgccacgat gctttcaagc gttggcggtg
    33301 gtgcggaaaa agggccgaat gaaaagtgga taatccccat cattaaagcg atggcaccga
    33361 acagcatgcc caatgaactg attttatttt gcgctatgat acccatctga cctccttgta
    33421 ggggatggca aaagcatatc aggaaacggg cggacgaaat tcttaaaaaa aaccgctaaa
    33481 gatagagcgt tagtgaaatt gcaaaggagc aacaatgaaa atttcccgcc tcggagaagc
    33541 gcccgattac cgcttctcgc tggctaatga gcgtactttt ctggcgtgga tccgtaccgc
    33601 actaggattt ctggcggcgg gggttgggct tgaccaactt gcgccagatt tcgccacgcc
    33661 cgtcattcgt gaactgctgg cgttgttgtt gtgtctgttt tccggcgggt tagcgatgta
    33721 tggctacctg cgctggttac gcaatgaaaa agcgatgcgt ctgaaagagg acctgcccta
    33781 caccaacagc ttattaatca ttagcttaat attgatggtc gtggcggtga tcgttatggg
    33841 actggtgttg tatgccggat agccgcaaag ccagacgcat tgccgaccca ggactgcagc
    33901 cggagcgcac atcactggcg tggtttcgta ccatgctggg ctacggcgcg ttgatggcgt
    33961 tggctatcaa acacaactgg caccaggcgg gcatgttatt ctggatttcg attggcattc
    34021 tcgccatcgt ggcgctgatc ctctggcact acacccgtaa tcgcaattta atggatgtca
    34081 cgaatagcga tttttcccaa tttcacgtag tccgtgacaa atttttgatc tccctcgcgg
    34141 tgttatctct cgcaatactg tttgctgtaa cgcatataca tcaacttatc gtatttattg
    34201 agagagtcgc atgacaggaa gtcaggtcat tgatgctgaa gaggacaggc ataaactggt
    34261 tgtagagtac aaggatgctc tgcaacctgc cgatttttat cacaatttca aacagcgggg
    34321 catccgctcc gtacagctca ttccctatct tgaatttgat gaccggggcg atctgacggc
    34381 cgcttcggtg acagcagagc tgtgggggaa atttttaatt gccctgttcg agtgttgggt
    34441 gcgggcagat atcagtcgta tctcgattga gctttttgac gccaccttac aaaaatggtg
    34501 tggcagcgaa aacccgcaac cgcgccgtga ctgccaggcg tgcgactggc atcgcctgtg
    34561 cccgcacgct cgccaggaga cgccagacag cgtgctttgt gcgggctatc aggcgtttta
    34621 ttcttactcc gcaccgcata tgcgggtgat gcgtgatctg ataaagcagc atcgttctcc
    34681 catggagctg atgacgatgc tgcggtgacg tgcgcttaaa cgggctgccc ctgatgcgac
    34741 atccgcgtcg ccagcggcag ccagcacagc acgatcaaca atcccattaa ggtcatcaac
    34801 aaccccaggc tgccctgacc ggtttgcggc aacatcgcag agagcgacgc cagcacgccg
    34861 gaaccaatgt tttgcagacc gccgaccagc gcgccagccg tgcccgccag gaaggggaac
    34921 ggctccatcg cgccgctggt cgccagcgga aacagcatcc cggcaccgaa aaagaacagc
    34981 gcggcgggaa cgagcagcgt ccagacattc atcacgccaa accagtcggg gatccacatc
    35041 agcaagccag ccagcaagca gcagataacc gactgccaca ttaacgtgga gaagcgttta
    35101 ttgggacgtc cggcaaacca tgcgccaaaa aacgctgccg gaatcggcag aataaacaaa
    35161 atactgaccg tcatactgct cagccctaac accgcgccca tcagcacgcc ggagcaggct
    35221 tcaaaggcgg caatcccggc cagaccgcca atcagcatca gcaaataaca gttaaaaccg
    35281 ctgttaccga aaagcgtttt ataactggta agcaggcgcg tgcgcggtgc atcgaccgga
    35341 cgcgtttccg gcatccagcg ggccatactg aaggtcacac cagcacaaag aaccaacaaa
    35401 aagagataac aggcgcgcca gttccacatc gtatccagca gaccgccgat tagcggtgcg
    35461 agcaacggac tgacgagaat ccccatgttt aacaggctgt tagcatggcg caactgtgtc
    35521 cgttcatata aatctcgcgg caaagtacgc gccattacgc cgccaacgcc ggtgcccatc
    35581 ccctgcatcg cgctggcggc aatcaacacc gtcaaactgg aggtcgtgac cgcgaccagc
    35641 gttgccagca taaaaatgga cattccgacg aggatcaccg gtcggcggcc cacgcggtcg
    35701 gaaatcgggc cataaaacag ctgtgagaca ccgtaagtca gcagataagc gcccattacg
    35761 ctctgcaccg ccccttcacg gacgttgaga tcgcgcgcca tatcggcaat agctggaata
    35821 taaatggttt gcgccatctg accgacggcc acgagtaata ccaacatcaa taacaaattg
    35881 acgtttcttt gccttttcat tatcacggat gcttttataa aaataattag aataagtcac
    35941 tacgcattcc cataaatgcg ggagaggaat ctatcacatt gatgaatgtt agccagatat
    36001 acgccaggaa tggggaattg tttagcggca gaatatgtaa acaaaagcgg caataaatgt
    36061 tgccgggagg gattacgtgc gaactgtaac cctctcccca aaggggcgag ggggaaacca
    36121 ccgttccctt tcagaaaatg agctaaacat tgcacgggac ggtcccctcg cccctttggg
    36181 gagagggcta gggtgagggg cacaggcagc gcaggtgcaa acattctgct ggtagaagaa
    36241 gcccccaact ccaacccgca cgctaaatat aaaaggggag cggttacccg ctcccctttg
    36301 gtgcgacttg aatctgaatt acttcaggta tttcagaaca gcatcaagca gttgcagtgc
    36361 tgcaaca
//
LOCUS       DAZGXZ010000040        35413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000040 DAZGXZ010000000
VERSION     DAZGXZ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.040"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..218)
                     /locus_tag="ACHKJF_002058"
     CDS             complement(<1..218)
                     /locus_tag="ACHKJF_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949417.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPDPLPF"
     gene            complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHKJF_002059"
     CDS             complement(421..822)
                     /gene="nikR"
                     /locus_tag="ACHKJF_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP0949418.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACHKJF_002060"
     CDS             complement(828..1634)
                     /gene="nikE"
                     /locus_tag="ACHKJF_002060"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP0949419.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACHKJF_002061"
     CDS             complement(1631..2395)
                     /gene="nikD"
                     /locus_tag="ACHKJF_002061"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP0949420.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACHKJF_002062"
     CDS             complement(2395..3228)
                     /gene="nikC"
                     /locus_tag="ACHKJF_002062"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP0949421.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMTATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACHKJF_002063"
     CDS             complement(3225..4169)
                     /gene="nikB"
                     /locus_tag="ACHKJF_002063"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP0949422.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACHKJF_002064"
     CDS             complement(4169..5743)
                     /gene="nikA"
                     /locus_tag="ACHKJF_002064"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0949423.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACHKJF_002065"
     CDS             complement(5854..6441)
                     /gene="acpT"
                     /locus_tag="ACHKJF_002065"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP0949424.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(6496..7545)
                     /gene="yhhT"
                     /locus_tag="ACHKJF_002066"
     CDS             complement(6496..7545)
                     /gene="yhhT"
                     /locus_tag="ACHKJF_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0949425.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            7677..8894
                     /gene="yhhS"
                     /locus_tag="ACHKJF_002067"
     CDS             7677..8894
                     /gene="yhhS"
                     /locus_tag="ACHKJF_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949426.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACHKJF_002068"
     CDS             complement(8898..9455)
                     /gene="dcrB"
                     /locus_tag="ACHKJF_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP0949427.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(9528..10193)
                     /gene="yhhQ"
                     /locus_tag="ACHKJF_002069"
     CDS             complement(9528..10193)
                     /gene="yhhQ"
                     /locus_tag="ACHKJF_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP0949428.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            10414..10659
                     /gene="tusA"
                     /locus_tag="ACHKJF_002070"
     CDS             10414..10659
                     /gene="tusA"
                     /locus_tag="ACHKJF_002070"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP0949429.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHKJF_002071"
     CDS             complement(10761..12959)
                     /gene="zntA"
                     /locus_tag="ACHKJF_002071"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP0949430.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTLVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALTIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEEKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(13033..13659)
                     /gene="yhhN"
                     /locus_tag="ACHKJF_002072"
     CDS             complement(13033..13659)
                     /gene="yhhN"
                     /locus_tag="ACHKJF_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP0949431.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            13800..14159
                     /gene="yhhM"
                     /locus_tag="ACHKJF_002073"
     CDS             13800..14159
                     /gene="yhhM"
                     /locus_tag="ACHKJF_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP0949432.1"
                     /translation="MSKPPLFFIVIICLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(14162..14431)
                     /gene="yhhL"
                     /locus_tag="ACHKJF_002074"
     CDS             complement(14162..14431)
                     /gene="yhhL"
                     /locus_tag="ACHKJF_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP0949433.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHKJF_002075"
     CDS             complement(14421..15017)
                     /gene="rsmD"
                     /locus_tag="ACHKJF_002075"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP0949434.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            15167..16660
                     /gene="ftsY"
                     /locus_tag="ACHKJF_002076"
     CDS             15167..16660
                     /gene="ftsY"
                     /locus_tag="ACHKJF_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP0949435.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNVDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            16663..17331
                     /gene="ftsE"
                     /locus_tag="ACHKJF_002077"
     CDS             16663..17331
                     /gene="ftsE"
                     /locus_tag="ACHKJF_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP0949436.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            17324..18382
                     /gene="ftsX"
                     /locus_tag="ACHKJF_002078"
     CDS             17324..18382
                     /gene="ftsX"
                     /locus_tag="ACHKJF_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP0949437.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            18627..19481
                     /gene="rpoH"
                     /locus_tag="ACHKJF_002079"
     CDS             18627..19481
                     /gene="rpoH"
                     /locus_tag="ACHKJF_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP0949438.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            19752..20855
                     /gene="livJ"
                     /locus_tag="ACHKJF_002080"
     CDS             19752..20855
                     /gene="livJ"
                     /locus_tag="ACHKJF_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP0949439.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            21006..21272
                     /gene="ataR"
                     /locus_tag="ACHKJF_002081"
     CDS             21006..21272
                     /gene="ataR"
                     /locus_tag="ACHKJF_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin AtaR"
                     /protein_id="HFP0949440.1"
                     /translation="MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRA
                     AEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKRAAKRLQGM"
     gene            21279..21806
                     /gene="ataT"
                     /locus_tag="ACHKJF_002082"
     CDS             21279..21806
                     /gene="ataT"
                     /locus_tag="ACHKJF_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     acetyltransferase AtaT"
                     /protein_id="HFP0949441.1"
                     /translation="MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKIL
                     RAYILCRNTPERQVMGYYTLSGSCFERVALPSKSKQKKIPYKNIPSVTLGRLAIDRSL
                     QGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
                     FFPTKSIELLFTQSD"
     gene            complement(21803..22186)
                     /gene="panM"
                     /locus_tag="ACHKJF_002083"
     CDS             complement(21803..22186)
                     /gene="panM"
                     /locus_tag="ACHKJF_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP0949442.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSTLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTQGVLDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGL
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            22610..23719
                     /gene="livK"
                     /locus_tag="ACHKJF_002084"
     CDS             22610..23719
                     /gene="livK"
                     /locus_tag="ACHKJF_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP0949443.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            23767..24693
                     /gene="livH"
                     /locus_tag="ACHKJF_002085"
     CDS             23767..24693
                     /gene="livH"
                     /locus_tag="ACHKJF_002085"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP0949444.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            24690..25967
                     /gene="livM"
                     /locus_tag="ACHKJF_002086"
     CDS             24690..25967
                     /gene="livM"
                     /locus_tag="ACHKJF_002086"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP0949445.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            25964..26731
                     /gene="livG"
                     /locus_tag="ACHKJF_002087"
     CDS             25964..26731
                     /gene="livG"
                     /locus_tag="ACHKJF_002087"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP0949446.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            26733..27446
                     /gene="livF"
                     /locus_tag="ACHKJF_002088"
     CDS             26733..27446
                     /gene="livF"
                     /locus_tag="ACHKJF_002088"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP0949447.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            27842..29158
                     /gene="ugpB"
                     /locus_tag="ACHKJF_002089"
     CDS             27842..29158
                     /gene="ugpB"
                     /locus_tag="ACHKJF_002089"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP0949448.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            29256..30143
                     /gene="ugpA"
                     /locus_tag="ACHKJF_002090"
     CDS             29256..30143
                     /gene="ugpA"
                     /locus_tag="ACHKJF_002090"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP0949449.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            30140..30985
                     /gene="ugpE"
                     /locus_tag="ACHKJF_002091"
     CDS             30140..30985
                     /gene="ugpE"
                     /locus_tag="ACHKJF_002091"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP0949450.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            30987..32057
                     /gene="ugpC"
                     /locus_tag="ACHKJF_002092"
     CDS             30987..32057
                     /gene="ugpC"
                     /locus_tag="ACHKJF_002092"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP0949451.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            32054..32797
                     /gene="ugpQ"
                     /locus_tag="ACHKJF_002093"
     CDS             32054..32797
                     /gene="ugpQ"
                     /locus_tag="ACHKJF_002093"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0949452.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTMRLGCVSLHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(32784..33224)
                     /gene="yhhA"
                     /locus_tag="ACHKJF_002094"
     CDS             complement(32784..33224)
                     /gene="yhhA"
                     /locus_tag="ACHKJF_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP0949453.1"
                     /translation="MKRLLILTALLPFAGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33345..35078
                     /gene="ggt"
                     /locus_tag="ACHKJF_002095"
     CDS             33345..35078
                     /gene="ggt"
                     /locus_tag="ACHKJF_002095"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP0949454.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRTKD
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(35116..35400)
                     /gene="yrhB"
                     /locus_tag="ACHKJF_002096"
     CDS             complement(35116..35400)
                     /gene="yrhB"
                     /locus_tag="ACHKJF_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP0949455.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         8251 a         9172 c         9913 g         8077 t
ORIGIN      
        1 atgaacggca gcgggtcggg cagggcgatg tcggtttcac cgggaaggac ctttgcaccc
       61 agcagcggat tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg
      121 ccggtggggg caccaatacg taccccggct gaaccctgaa caatgctacc gccatactgc
      181 gtcatgtcac cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttctattatt
      241 ttaatgacag tatcttcata cgaaaaagcg aagaaaaact tgtggtcaca atgaattgaa
      301 gatttttagg aatatattta gaacgttaca aaagagaagg ttcggatagg gtatttagag
      361 aggaatcagg tgtgctagtg gccctttgtt gatcgttcac caaaggaccc aataatagac
      421 tcaatcttcc ttcggcaagc actgcaaatg cccgtgccgc acgccgcgct gggcgataac
      481 gtcatcggca aaatgctgca cgtcgcccat atcacctttc agcacggcga tttccagaca
      541 gtcgtcgtga ttgatatgca catgcagcgt ggcgacggag agatcgtggt gatggtgctg
      601 ggtggagaca atgcggctgg ctaagtcgcg tttttcgtgt tcatacacat aggagagcac
      661 cgcgaaacct tgcgtgccgt gctgctgagt ggcctcctgg gccagagcgc tacgcagaat
      721 atcgcggata gcttcggaac gattgttata accacgacgc tggctcaggc tgtccagcgt
      781 ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc atttgcgtta aaccttttct
      841 gtggtgcgac ggcgcacggg gaatgcgggt aataccgcgt tttgtagcac acgtccggcg
      901 tcagaggaaa aggttaattt atctcccacc acctgggttt cgacgatttg tccgttgtcc
      961 atcaccatca cccgctgaca gaagcgttcc accaggcgta agtcgtgggt gatgaacagg
     1021 caggcggtgc caaactgttg ttgtagcttt ttcagcaggc gaatgacgcc cgcctgtaac
     1081 acgagatcaa ggttagaaac ggcttcatcc agaatcagta gtttcggttc gaccgccagc
     1141 gcgcgagcca ggcagacgcg ctggagctgg ccgccgctta actgcggtgg gcgtttgtcg
     1201 agaacgctgt catcgagatc gaccgccttc agcatttcgc tggcgcgcgc cagttgttcg
     1261 gattttttca gtgatagcag gtggcgcatc ggttcacgca ggatctcgcg cacggttttg
     1321 cgcggattca cggcgctgat ggagtcctga aataccatct gaatatcgcg gcggaacgct
     1381 ttacgctggg cgcgattgag tttcgccagc ggttcgccgc gccagctaat attcccttgt
     1441 gagggagact ctaagcctac cagcagccgc gcgagggtgc tttttccgca gccgctgcgt
     1501 cccagcaggg cgacggtttc gccgcttttc agggtcaggg aaacgttatt cagcaccgcc
     1561 tgatgttgat gttttccgct aaatccaccg tgggcatagt gatgggaaag gccgcagacg
     1621 ttaagtaagg tcatgatgcc agctccatac cgtagagggc gagatgagcg gaaaccaggc
     1681 tgcgcgtcac cgtatgtttg ggggcgttaa acagcgtttc tacatcgccc tgttcgacaa
     1741 ttttaccttg tgacatcacc gccacgtcat ccgccagacg cgccacaacg cccatatcat
     1801 gggtgaccag cagcattccc ggcgcttgtt tttgcataat gctttccagc agatcgagga
     1861 tgcgcgcctg tgctaccacg tcgaggtcgg tggtcggttc atcggcgatg ataaacggtg
     1921 attcacacag caccgccatc gcaatcatca tgcgctgcaa catgccgccg ctcatctcga
     1981 acgggtacag cttcagcacg cgcgcggcgt tttccagccc caccgcttct atggcagcgg
     2041 taagcgtagc gtcatcggcg ggtttcccta acgccaggca ggtttcccgc gcgtgggtgt
     2101 gcatggtgtg cagtggatta aaggcgctgc gcgggttctg catgatggtg gcaattttga
     2161 tgccgcgcag ggcgcaaggc gaaaccggtt tgccatcggc taaaatttcc cccgccgtct
     2221 ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca ggttaatgat ttcccgctgc
     2281 cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag ggttaacgat acaccgtgta
     2341 ccagcggctg cgcggcctgt agcgcgatat tacgtagttc aatctgttgc ggcattagtg
     2401 tgcgtgctcc gtcaccagat gagggtccag atgatcgcgc agtgcgtcac ccaccaggtt
     2461 aaaggccatc acgctgataa acagcgccag ccccggccag aacatttgca gcggctgggt
     2521 ccagatatac tggcgcgcgt cgttaatcat cacgccccat tcggcggtcg gcgcggtcac
     2581 accgaggcca aggaaagaca tccccgcgac gtgcagcatc atatggccga tatccagcgt
     2641 tgccagcacc agcagcgaag ggatcaccgc gcctgccaga tgatcgacaa acacccgcac
     2701 atggcccgca ccggaaagcc gtgacgccag cacaaactcg cgttggcgta gtgaaatcac
     2761 caggctgcgc accatgcgtg cataccacgc ccagtgcgac agggcgatgg cgataattac
     2821 gttggtcagc ccggtgccga gcacgccaac cataaagaac gacagaatcg aggtcgggaa
     2881 ggtcataaac atatcggcga cgcgcatggt ggcctgatca acgcgcccgc caatcaaccc
     2941 ggcgctgccg ccaataacca gccctaatgt cagcaccagc agcaggcagg ccattaccga
     3001 accgagcgac acgcgggtcg ctgtcatcag ccgcgagaaa atatcgcgac ctaagtgatc
     3061 ggtgcccagc cagtgctgcg catccggcga aagcaggcgc gacggcaaat caatcgcctg
     3121 ggggtcatac ggcagccacc actggctggt gagcgcaatc agcgccagca gggcgataat
     3181 gatcagcgcc aggcgtaccg accagcggga agagaggaaa aagttcacgc gtgcgctcct
     3241 tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca aatcgacaat caaattacag
     3301 acaacaaaaa ccaccaccat catcagcgta aagcactgaa tcaccggata gtcacggtta
     3361 aaaatcgccg acaccgcata gcgcccgacg cccggccagg caaagatgtt ttcgataatc
     3421 atcgtcccgc cgatcagttc gccgatgtgc atccccacgg cggtgatcat cggcagcgag
     3481 gcattacgca gaatatggcg gcgttcggtc tgtttgtcgt tcaggccgcg cagacgcgcc
     3541 caggtgacgt gacgctgacc ggcgacgtcc agcatactgg cgcgcagtaa acgcgcgtta
     3601 atcgccagcg acataaaggc aatggaaacc gcaggcaaaa tgatgtgctg ccagccgccg
     3661 taacccatcg cgggtagcca ttgcagatac accgaaaacg ccatcaccag taaaaacgcc
     3721 agccagaagt taggcatcga cacgccaaga aacgcgataa agcgcacggc gaaatccggc
     3781 aaacgatcgc ggtggcgcgc cgcccagata ccgagcggta cggaagtgag cagaattaat
     3841 accagcgccg cacctgcaag ttccagcgtg gcgggcagga agttcagcat atcgtccagt
     3901 accgggcgtt ggctggcgaa tgagatacca aagtcaagat gcagcgcctt ccacaaccag
     3961 gtaccgtact ggacgtacag cggctgatcc agcccgagca tggtgcgggt agaggccagc
     4021 atctccggcg tcggcggcag gttagataaa cgcagataat cgagcgccgg gtcgccggta
     4081 ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga gcaccatcgg gatcagcagc
     4141 agaaagcggc gtaatacgta acgcaacatt aaggtttcac cggtttaatc tgttcgaacg
     4201 gaatttcggt ggcgatcggc gcgtagggga tattacccag ctccggtttt gataccacca
     4261 tcatcgagat gtagctgata ggcagataaa ccgcctcgtc atgcagacgg gtcagaatgt
     4321 cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc cagcacttcg ccgatctctt
     4381 tatcaatcag cggcttatcg gctaatcctt gctgtgcctg gaagtcagcg tgcgacggta
     4441 cgcgcattga actcaggaag gcgtgtggat cgtatggcgc gccccaggtg cggtggaaaa
     4501 tcatgccaaa acgaccgtcg cgctgacgag catagatact gctctcttct tcgccaatca
     4561 gcgagacatc tgcgccaatc tggcgcatat cagcctgaat gatttccgcc atcgatttgc
     4621 ttaacgcatc ggtgccgatg aacgacagtt caatgcgcag cggctgacca tttttctcgc
     4681 ggatgtcttt gcccgcaggc agcgtccaac cggctttttc cagcaacgct ttcgctttct
     4741 gcggatcgta ctggcgcggt ttcaggccga gattagcgta aggcacagaa ggggcaaaca
     4801 gggtgtcggc gacctgctgg gtgccatata acgcgttatc aatcagcgat tttttgttta
     4861 ccgcgtaatt aagagcttca cgtactgcca gctcgttggt gggggctttg gcggtattga
     4921 gcgccagcat cacggtttcg atcggctgtg acagttgggt gtggtaagcc ggattctggc
     4981 taaagcgggc gaaggtatcg agcggtaata acccttcgtt tccgtacagc aggtcgatat
     5041 cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg gatgacgtta aaggtgatct
     5101 ttttaatcgc tggcttttcg ccccagtagt tttcgttacg gacgaagaca tcgtactgat
     5161 tcagtttcga ttcctgcaaa atccatggtc cggtgccaat cggcgcttta attccgttca
     5221 tggtttcatg gtttttaaac tgcgagggag cgataaaacg gaaaggacgg ggcagggcca
     5281 gttcttgcag gaaaggatag taggcgcttt tcagggtaat ttgcagctct gttttactga
     5341 gtgctttaac atcaacaatc tggtttgcca gctccagcca ggcgtgacgt tgacggttat
     5401 cgagcactgc gcggaagttt tctgccgccg cctcggcatc gaacggttca ccgttggaga
     5461 atttcacgtc atcacgcagg gtgaaggtcc aggttttacc atcttctgaa tgagtccagc
     5521 tttttgccag ccacgggatc accgaaccgt ctgcctgata tttcaccaat ggttcataaa
     5581 ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg cgggtttagt ggcccgacat
     5641 tcaccggcca ggcggtagtg atttcatctg gtgcagcggc atggacgata aaagacgcac
     5701 aagccagcag cgcaaataga gtgcggcgga gtgtggagag catgataacc ccaatggatt
     5761 aaaatagatg gcggaaataa gtatgacgat tttaaatatt cgtcatactg attacctgtt
     5821 ctggatcaat agatgggcag tcgggtgggc ggatcagtta actgaatcga tccattgcac
     5881 actgtcggcg gtgagcgtaa agggggtggg ggtgcagatc gccaggctta aattttcaag
     5941 ctgacaatgg ctgactgaca gcgaggagtg ataggtgctg tctacgctga cgatttgcca
     6001 ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc gtccagatgc gccagaacat
     6061 ttccagttgc tgatcaggat gcacggcgtc catctcagcg tgttccccga ggctgaatac
     6121 ggcgttcgcc agccagcgcc agttggcgcg cgggcgaatc acttcgatat cgcagccgac
     6181 ttcgccttca tcactcaaca gcagggcgat atcgtcaccg ctatggctta agttgaacca
     6241 tagcggcatt tccggcgcaa atgcaggttt gccttgttcg ccatagatga tctccggtag
     6301 cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc cagcgttcgc gtcgtggacc
     6361 ttgcggtgct tgctcgcgta aacccggtgg cagtggagct gcgcttaagg tcgaaacttt
     6421 ccccagaact atccgataca tatcagggcc aacgtttaat ggaaaatgaa agtgcgtatc
     6481 gtatcacttg tcgcctcatc ccggtaaccg acttttcggt ctgcctggcc ccagtaaaat
     6541 cgccagtttg ctaccgcctt tggtggtttc catccagatt ttacacacgc tggttaatgg
     6601 caccgaaagt agcatcccta ccgggccgag cagccatccc cagattaaca atgaaagaaa
     6661 taccaccatg gtggacatcc ccaggcgatg gcccatcatc cgtggctcta aaatattgcc
     6721 gatgaccata tggacaacta aaaacaatgc gccgaccaga atacattcgt aaataccatt
     6781 aaacagcagc acctgaatca ttggcggtac ggcggaaatt accgcgccga tattgggcac
     6841 gtagttaagc aaaaacgcca gtactgccca catcagcgca aactgtaccc ccatcagctc
     6901 cagccccagc cagacgatga cacctgtcca taaactgagt agcgtcttca atgcaagata
     6961 gtgcgaaacg cctttaagtg cgcggtgtaa tcccgcgatg tgaatctgtg gattattcag
     7021 cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac agcataaaaa ctacggtcat
     7081 caccagcaaa agcacgctcg ccattgcccc ggaaagcccg gtcattagcg ctgtggtgaa
     7141 ggtaaccact ttttccgagt ccatccgctg cagcattcgc tccggcgaca tatgcaaatt
     7201 aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc gtcagctcct tattaaactt
     7261 cggcagcata gagataaatt cgttaaacga tgccgccagt acgccgacca gcgcggttag
     7321 tgcgatcagc atcaccacca ctacaatcgt aatggcaacg gggcgttgta ctccccgacg
     7381 aataaaccag gtgacgagcg ggttgaggac gatggcaaaa aacagcgcca gtaacagctg
     7441 cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc actaccagcg aggccagctt
     7501 gagcagaatg tgcatgcccg ttttatcggg ttgaggggtt tccattgggg cttccttgtg
     7561 actttttgta ttaagtgtag cgggagtcac gccagcaata ttctgattct tactgctgat
     7621 tttcacgtca gcacatggta aaaatgaaac actgttgtaa aaatgtggtg atcctcatgc
     7681 ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc attgtctcca
     7741 ttgtcatgtt taacttcgcc agctacctca ccatcgggtt gccgctcgct gtattaccgg
     7801 gctatgtcca tgatgtgatg ggctttagcg ctttctgggc aggattggtt atcagcctgc
     7861 aatatttcgc caccttgctg agccgccctc atgccggacg ttatgccgat ttgctgggac
     7921 ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg gggtatctga
     7981 cggcaggatt aaccgccagt ctgcctgtca tcagcctgtt attactttgc ctggggcgcg
     8041 tcatccttgg gattgggcaa agttttgccg gaacgggatc gaccctgtgg ggcgttggcg
     8101 tggttggctc gctgcatatc gggcgggtga tttcgtggaa cggcattgtc acttacgggg
     8161 cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca ctggggcggc ttgcaggcgt
     8221 tagcgttaat cattatgggc gtggcgctgg tggccatttt gttggcgatc ccgcgtccga
     8281 cggtaaaagc cagtaaaggc aaaccgctgc cgtttcgcgc ggtgcttggg agcgtctggc
     8341 tgtacggtat ggcgctggca ctggcttccg ccggatttgg cgtcatcgcc acctttatca
     8401 cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg ctgtttagct
     8461 gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat taaccgtatc ggtggcttaa
     8521 acgtagcgat gatttgcttt agcgttgaga taatcggcct gctactggtt ggcgtggcga
     8581 ctatgccgtg gatggcgaaa atcggcgtct tactggcggg ggccgggttt tcgctggtgt
     8641 tcccggcatt gggtgtagtg gcggtaaaag cggttccgca gcaaaatcag ggggcggcgc
     8701 tggcaactta caccgtattt atggatttat cgcttggcgt gactggacca ctggctgggc
     8761 tggtgatgag ctgggcgggc gtaccggtga tttatctggc ggcggcggga ctggtcgcaa
     8821 tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc cctgaggccg
     8881 cctcatcatc ttaaaactta ctgaataacc agcgtattaa tgatgttttc tgcggtggtc
     8941 tgcgcttttt gctgatcgtc agcgggcagc gtaatttgca tggtcagcag ttgattaccc
     9001 acgttaccca gaataacgga agagtacgcc gtctggcctt tcgcggagat aatactgtct
     9061 aactgctgca ttttgtgacc tttcagctca atggctttat tggttaccac ttgcagctgc
     9121 ggatcgcggc tacgttgctg atcttccaga cgcttcgcca gcaccgccag atcttctttc
     9181 ggatcatcgc ccatgatgac gatgactgct ttctgcccgg tggcgtcgga ccagacatgc
     9241 atgttattgg cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc tggcagcgag
     9301 aaacttaact tgccatcaag caggttgacg ggattcccgg tagcgttact ttccgcgacc
     9361 gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc cataaccagc
     9421 aggccaattc cgacatattt aaccagattg cgcattgact tcttcctttc gataaacggc
     9481 cataacggct cattcatcca tcttatcaca actctgataa cgaaccttta actcgcctgc
     9541 aaagcgttga tttcggattt atctgccagt cttttcaaca gcatattgag taatacgcca
     9601 tacattggca ggaagaaaac gatactgatt aacactttga aacagtaatc gaccagcgcg
     9661 atttccatcc agtgttcagc cataaaggca tccgggctac gccagaaggc aatgaagaaa
     9721 aaggccagcg tgtcactgac gttaccgaac agtgtggacg ctgtcggtgc cagccaccag
     9781 cggcgactct gacgcaggcg gttaaaaacg tgcacgtcga ggatctgtcc cagcgcgtag
     9841 gccatgaaac tggcggtggc gatacgggcg acaaacaggt tgaagtgggc gagtgcgccg
     9901 aatccctgcc aggaacccat atagaatagc gacgagatga cgtaggagat taataacgca
     9961 gggatcatta ccgcgaagat aatgcgtcgg gccagcggtg cgccaaaaat acgcacggtc
    10021 aggtcggtag caagaaaaat aaacggaaag ctaaacgcgc cccaggtggt atggaaacca
    10081 aaaatggaga cgggaagctg aaccagatag ttactggagg tgatcaccag cagatgaaat
    10141 aacgataacc agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt catattgtac
    10201 ctttttgatt aaccattggg gtgagggaac ccaatacgta cgacacgtct gttaccttaa
    10261 cataataacg tgcttaccct tttttcgagc cgccgcatga tactgcttta cgttgacaat
    10321 gcaatggtta attttcacgc aatcgttaac ctggtttgct tacggactca cagggcgtaa
    10381 actagcgccg tttttttatg tgatgagaag aaaatgaccg atctcttttc cagccctgac
    10441 cacacactcg acgcacttgg cctgcgctgc ccggaacccg taatgatggt gcgcaaaact
    10501 gtgcgcaata tgcagcccgg cgaaacgttg ctgattatcg ccgacgatcc ggccactacc
    10561 cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgcgaa agagacggat
    10621 ggactgcctt atcgttattt gattcgtaaa ggcggttgat gggggctgat tggcttcgat
    10681 gccgcctttt cccctcaccc taaccctctc cccagagggg cgaggggacc gatcgcgctc
    10741 aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc ttaacgcatt cgctgtcacc
    10801 agcaccgtcg cacccgtatc tgccagcacc gccagccata gtccggtcat ccctaacagc
    10861 gtggtgacga ggaagatccc tttcagtccc agcgcaatgg tgatgttctg gcggatattg
    10921 gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg gttatgggtt
    10981 aatgctgcat cggcggtttc cagcgccacg tctgtgccgc tgcccattgc aatcccaatg
    11041 gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac cgaccatcgc cagcggcgca
    11101 tgttgattca gcttggtcac cgctttgact ttctcttccg gcaataggcc cgctttaaac
    11161 tccagcccca gctccccggc gattgccgcc gctgcgcgtg gattatcgcc ggtgaggatc
    11221 actcctttaa ctcccagcgc gttcagttca ctgatggcag ttgcagcgtc ggcgcgcagg
    11281 gtatcctgca atgcaatgat acccagcacg tcatcattac gtactaccag cacaaccgtt
    11341 tgcccggcgc tttccagttc attaatcaaa ccagcaaatg catcagcggg atgtttcccg
    11401 gcagcgcaaa tcaatacgcg ctcaccgtta acctgcgctt caatgccaga cccgaccagc
    11461 gcccgctgtg actcggcggc gggaatggtc agtgcagcaa cctgtgcttc tcgcacgatg
    11521 gcctgcgcca gtggatgcgt cgcgccttgc tcgaccgccg ccgccagtgt cagcagttca
    11581 gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc gacggtcagc
    11641 gtaccggttt tatcaaacgc caccagggta acacgaccca gttgttccag cgccgctccg
    11701 cctttaatta acgccccacg acgcgctgct gctgccagcc cggaggtaat cgccgcgggc
    11761 gtggagataa ctaacgcgca cgggcagcca atcagcagca gcgtcaggcc tttataaatc
    11821 cactcctgcc agctggcggc aaacagcagc ggcggcacca gcgttaccag cagggcgacg
    11881 gccataatcg ctggcgtata aatacggctg aaacggtcga taaaccgctc aatgggggcg
    11941 cggcgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct ggcacccggt
    12001 tctgacagca cttccagcgt taccagacgg tctacgctgg tggctccagc aggaacttta
    12061 tcgcccgtcg cgcgctccac cggaatggat tctccggtca gggcgctttc atcaaaactg
    12121 gcaaacggtg agagcagttt accgtcggca ggcaaacgcc cacctgcggc gacttcaatc
    12181 acatcgccag gccgcaggct gttaatcgcc acctcttccc gctcaccgtt acgcaggcgc
    12241 gtggcggttt ctggtttcag cgccattaac gcgctaacgc cctgacgcgc gcggctggcg
    12301 gcccagcctt ccagtcgttc accaatcaaa aacagcagca acaccatcgc agcttcagcc
    12361 gttgcgccaa taaacagtgc accaatagcg gctacgctca ttaaggtttc aatggcgaag
    12421 tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa tcgggtacag cccaaccagc
    12481 gtggtcgcga taaacgccag ttgcccgaac ggatgattga actgctccag accccagctg
    12541 attgccatca tcacgattag tgtaatcagc ggcagattct ctttcaggcg cgatgcttgt
    12601 ggctcgtcgg cggcctgctc atcgcgcagg gaatagcccg ctttttgcac cgcagattca
    12661 acttgtgcac gaatgtcatt gtcggcatca accaccagtt tttcggtggc gaacagtacc
    12721 tgcacctgat tcacacctgc aagctggcgc acggcatttt ctaccttgcg cgcacaggcg
    12781 gcgcagtcca tgccgctgac tttccagcta tagcgggtgc cggagacgtt ttcagagagc
    12841 gttggcgagc tggagcatgc gccgtcgcag caacagtcgt tggcgttctg taccgtggtt
    12901 agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg tgtcgacatg
    12961 gcatcctccg gttaagtttt ttctcattaa ccgaaggata cactctggag tcgactccag
    13021 agtcaagttt tatcagagat acagtgagcg gacgatgagg aagtgaccgg caaagtagca
    13081 ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga tagtggctcc ccagccagac
    13141 aaagttactg ataaacagca acgaagcgcc aacaaacgca gagagcgccg gagcggtcgg
    13201 acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc cgataaacgt
    13261 gcagataggc caacgatact cttccagccg cgtccagata atcgccagta acagcgcacc
    13321 cagaaccagc aacaccagtg gcagaggcca gaagaaagag agcgtcatct gactggcgaa
    13381 atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca tcagacgttg
    13441 acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagccctg ccagcaccag
    13501 atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt aacagcagga gaagaagggt
    13561 taacggttta aacacccagc gttgccaggt tggcccacga tacgatgcat ccacagatag
    13621 ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt gaattcgtta
    13681 ttttttcgta agcggaatgc ctacggtctt gcttccagtt tagtggcgag ggtggacgga
    13741 tgacaacacc ataactggca gggtatgctt atttccgcat tttccgatga gggataaaga
    13801 tgagcaaacc acctcttttc tttattgtta tcatttgctt aattgtcgtc gccgcatcgt
    13861 ttcgttttat gcaacagcga cgggaaaaag ctgataatga tatggctccg ctccagcaaa
    13921 agctggtggt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt tcgcgccagc
    13981 aggaagtgac tccggcaggc accagtatgc gctatgaggc aagcttcaaa ccgcaaagcg
    14041 gaggaatgga gcagacgttt cgcctcgacg cccagcagta ccacgccctg acagtgggcg
    14101 ataaaggtac gctgagctat aaaggaacgc gctttgtcag ctttgtaggc gaacaataag
    14161 gttatttttt aactttaaat ttcttctgcc agaccagcag ttcaaacacg ccgaaaagga
    14221 aaatccgcac cttttcggcg gtggtcatct gcgggccatc tttcggtaaa gtggatttca
    14281 atagcgccag ttgcatacca tgcatcagca cggtaaaaat cagcgccacg ttaacgaaaa
    14341 tattcagcgg gcgcgggaag ggatgcacca gattgaggat taaaaatccc caaacgcaga
    14401 gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc gcgttgatac
    14461 agccgataag ccacctgacc cgccactttt tcccgatgta atgaccagtt tgccggaaca
    14521 gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc agccagccag
    14581 ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa cggtggatcg
    14641 acaaacacga tattatgcgg tgtgcctttt tgcgccagga atgacattgc gttgctgttc
    14701 accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg ctgagaaact
    14761 gcgcgatcca tctcaatcaa cgtggccccc gcagcgtagc gcgataacgc ttccagcccc
    14821 agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt caacaatgac cggagccagc
    14881 cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg gctatcagga
    14941 accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc gctgccggaa
    15001 tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac ttgcaggcgg
    15061 tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc tatgtacata
    15121 gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg cgaaagaaaa
    15181 aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc cggaaaaaga
    15241 gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc aagagcctgt
    15301 gaaggcttct gaacaagccg tcgaagagca gccgcaggcg catactgaag ccgaggcgga
    15361 aacttttgct gccgacgttg tggaagtcac tgaacaggtt gctgaaagtg aaaaagcgca
    15421 gcctgaagcg gaagtcgttg cacagccgga accggtcgta gaagaaacgc cggagccagt
    15481 ggctatcgag cgtgaagaac tgccgttgcc ggaagacgtc aacgccgaag aggtttcgcc
    15541 agaagagtgg caggctgaag cggaaaccgt agagattgtc gaagcggcgg aagaagaagc
    15601 ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggctg ccgaagcggc
    15661 agaagaggcg gtgatggtgg ttcctccggt agaagagcag ccggtggaag aaatcgctca
    15721 ggagcaggaa aaaccgacca aagaaggttt cttcgcgcgc ctgaaacgca gcctgttaaa
    15781 aaccaaagaa aatctcggtt ccggatttat cagcctgttc cgcggtaaaa aaatcgacga
    15841 tgatctgttt gaggagctgg aagaacagct attgatcgcc gatgtgggcg tggaaaccac
    15901 acgtaaaatt atcaccaatc tgacggaagg cgcatcccgc aagcagcttc gtgatgccga
    15961 ggcgctctat ggcctgctga aagaagagat gggcgagatt ctggcgaaag tcgatgagcc
    16021 gctgaatgtt gaaggtaaaa cgccgttcgt gatcctgatg gtgggcgtca acggtgtggg
    16081 taaaaccacg acgattggta agctggcgcg tcagtttgag cagcagggta aatcggtgat
    16141 gctggcggcg ggcgatactt tccgtgcagc tgcggttgaa cagcttcagg tctggggtca
    16201 gcgtaacaat atcccggtga ttgcccagca tactggtgcg gattccgctt cggttatctt
    16261 cgacgccatt caggctgcga aggcgcgtaa tgttgacgta ctgattgccg ataccgccgg
    16321 acgcctgcag aacaaatcgc acctgatgga agagttgaag aaaatcgtcc gcgtgatgaa
    16381 gaaactcgac gttgaagcgc cgcatgaagt tatgctgact atcgatgcca gcaccgggca
    16441 gaacgcggta agccaggcca aactgttcca tgaagctgtt ggcttaaccg gcatcacgct
    16501 aacgaaactg gacggcacgg cgaaaggcgg ggtaattttc tcggtggctg accagtttgg
    16561 tatccctatc cgctacattg gtgtcggcga acgtattgag gatttgcgtc cgtttaaggc
    16621 ggacgacttt atagaggcac tttttgcccg agaggattaa caatgattcg ctttgaacat
    16681 gtcagcaagg cttatctcgg tgggagacag gcgctgcagg gcgttacgtt ccatatgcag
    16741 ccgggtgaga tggcgtttct gaccggtcat tccggcgcag ggaaaagtac cctcctgaag
    16801 ctgatctgtg ggattgagcg gcccagcgcc gggaaaatct ggtttagcgg ccatgacatc
    16861 acgcgtctga aaaaccgtga agttccgttt ctgcgccgcc agattggcat gattttccag
    16921 gatcaccatc tactgatgga ccgtactgtc tacgataacg tggcaatccc gctgattatc
    16981 gccggtgcca gcggtgacga tattcgtcgc cgggtgtcgg cggcactgga taaagtcggg
    17041 ctactggaca aagcgaagaa cttccctatt cagctttctg gcggtgaaca acagcgtgtt
    17101 ggcattgccc gcgcggtggt gaacaagccc gcggtactgc tggcggacga accgactggt
    17161 aacctggacg acgcgttgtc ggaagggatt ctacgtctgt ttgaagagtt taaccgcgtt
    17221 ggggtaaccg tattgatggc aacgcacgac atcaacctga tctcgcggcg ttcctatcgc
    17281 atgctcaccc tgagcgatgg tcacttgcat ggaggcgtgg gccatgaata agcgcgatgc
    17341 aatcaatcat attcggcagt ttggcgggcg tctggatcgc ttccgtaaat cggtcggcgg
    17401 ctctggcgac ggcggtcgta acgcgccaaa acgcgcgaaa tcctcgccaa aaccggtaaa
    17461 tcgcaaaacc aacgttttca acgaacaggt gcgctatgcc ttccacggcg cattgcagga
    17521 tctgaaaagc aaaccgttcg ccacgttttt aacggtgatg gttatcgcca tctccctgac
    17581 gctgcccagc gtctgttata tggtgtacaa aaacgttaac caggcggcga cgcaatatta
    17641 tccgtcaccg caaatcactg tttacttgca aaaaacgctg gacgatgacg ccgcagcagg
    17701 cgtggtagca cagctacagg ccgagcaggg cgtggagaaa gtgaactatc tttctcgtga
    17761 agacgcactg ggcgagttcc gtaactggtc tggttttggt ggtgcgctgg atatgctgga
    17821 agaaaacccg cttccggcag tggcggtggt gatcccgaaa ctcgattttc aggggacgga
    17881 atcactgaat acgctgcgtg atcgtatcac ccagattaac ggcattgacg aagtgcggat
    17941 ggatgacagc tggtttgccc gtctggcggc gttgaccggg ctggtcgggc gcgtttccgc
    18001 gatgatcggc gtgttgatgg tggcagcagt gttcctcgtc atcggcaaca gtgtgcgtct
    18061 gagcatcttt gctcgccgtg actccattaa cgtgcagaaa ctgattggtg cgacggatgg
    18121 tttcatcctg cgcccgttcc tgtacggagg cgcattgctg ggattttctg gcgcattgct
    18181 gtcattaatt ttgtcagaaa ttctggtact gcgtttgtca tcggcggttg cagaagtggc
    18241 acaggttttc ggaacgaagt ttgatatcaa tggtttatcg ttcgatgaat gcctgctatt
    18301 gctgctggta tgctcgatga ttggctgggt ggcagcgtgg cttgccacgg tacaacattt
    18361 acgccacttt acgcctgaat aataaaagcg tgttatactc tttccctgca atgggttccg
    18421 tagcagggaa agagatcccg ttgtctcttc ccggtatttc atctctatgt cacattttgt
    18481 gcgtaattta ttcacaagct tgcattgaac ttgtggataa aatcacggtc tgataaaaca
    18541 gtgaatgata acctcgttgc tcttaagctc tggcacagtt gttgctacca ctgaagcgcc
    18601 agaagatatc gattgagagg atttgaatga ctgacaaaat gcaaagttta gctttagccc
    18661 cagttggcaa cctggattcc tacatccggg cagctaacgc gtggccgatg ttgtcggctg
    18721 acgaggagcg ggcgctggct gaaaagctgc attaccatgg cgatctggaa gcagctaaaa
    18781 cgctgatcct gtctcacctg cggtttgttg ttcatattgc tcgtaattat gcgggctatg
    18841 gcctgccaca ggcggatttg attcaggaag gtaacatcgg cctgatgaaa gcagtgcgcc
    18901 gtttcaaccc ggaagtgggt gtgcgcctgg tctccttcgc cgtccactgg atcaaagcag
    18961 agatccacga atacgttctg cgtaactggc gtatcgtcaa agttgcgacc accaaagcgc
    19021 agcgcaaact gttcttcaac ctgcgtaaaa ccaagcagcg tctgggctgg tttaaccagg
    19081 atgaagtcga aatggtggcc cgtgaactgg gcgtaaccag caaagacgta cgtgagatgg
    19141 aatcacgtat ggcggcacaa gacatgacct ttgacctgtc ttccgacgac gattccgaca
    19201 gccagccgat ggcaccggtg ctctatctgc aggataaatc atctaacttt gccgacggca
    19261 tcgaagatga taactgggaa gagcaggcgg caaaccgtct gaccgacgcg atgcagggtc
    19321 tggacgaacg cagccaggac atcatccgcg cgcgctggct ggacgaagac aacaagtcca
    19381 cattgcagga actggctgac cgttacggcg tttccgctga gcgtgtgcgc cagctggaaa
    19441 agaatgcgat gaaaaaactg cgcgctgcta tagaagcgta atttccgcta ttaagcagag
    19501 aaccctggat gcgagtccgg ggtttttgtt ttttgggcct ctacaataat caatttcccc
    19561 tccggcaaaa cgccaatccc cacgcagatt gttaataaac tgtcaaaata gctattccaa
    19621 tatcataaaa atcgggtatg ttttagcaga gtatgctgct aaagcacggg tagtcatgca
    19681 taaaacgaaa taaagtgctg aacaacaaca tcacaacaca cgtaataacc agaagaatgg
    19741 ggattctcag gatgaacata aagggtaaag cgttactggc aggatgtatc gcgctggcat
    19801 tcagcaatat ggctctggca gaagatatta aagtcgcggt cgtgggcgca atgtccggtc
    19861 cggttgcgca gtacggtgac caggagttta ccggcgcaga gcaggcggtt gcggatatca
    19921 acgctaaagg cggcattaaa ggcaacaaac tgcaaatcgt aaaatatgac gatgcctgtg
    19981 acccgaaaca ggcggttgcg gtggcgaaca aagtcgttaa cgacggcatt aaatatgtga
    20041 ttggtcacct ctgttcttca tcaacgcagc ctgcgtctga catctacgaa gacgaaggca
    20101 ttttaatgat caccccagcg gcaaccgcgc cggagctgac cgcccgtggc tatcagctga
    20161 tcctgcgcac caccggcctg gactccgacc aggggccgac ggcggcgaaa tatattcttg
    20221 agaaagtgaa accgcagcgt attgctatcg ttcacgacaa acagcaatac ggcgaaggtc
    20281 tggcacgagc ggtgcaggac ggcctgaaga aaggcaatgc taacgtggtg ttctttgatg
    20341 gtatcaccgc cggggaaaaa gatttctcaa cgctggtggc gcgtctgaag aaagagaata
    20401 tcgacttcgt ttactacggt ggttatcacc cggaaatggg gcaaatcctg cgtcaggcac
    20461 gcgcggcggg gctgaaaact cagtttatgg ggccggaagg tgtggctaac gtttcgctgt
    20521 ctaacattgc gggcgaatca gcggaagggc tgctggtgac caagccgaag aactacgatc
    20581 aggttccggc aaacaaaccc attgttgacg cgatcaaagc gaaaaaacag gacccaagcg
    20641 gcgcattcgt ttggaccacc tacgccgcgc tgcaatcttt gcaggcgggc ctgaatcagt
    20701 ctgacgatcc ggctgaaatc gccaaatacc tgaaagcgaa ctccgtggat accgtaatgg
    20761 gaccgctgac ctgggatgag aaaggcgatc tgaaaggctt tgagttcggc gtatttgact
    20821 ggcacgccaa cggcacggcg accgatgcga agtaattgtt agataaatga aaatgcccgg
    20881 tgcgcttaac gtccatcggg gtttttttgt gtttgtcgaa atggttgtag aaagattaaa
    20941 caatccagga tatgcttatt gtacggtgat atgccgtaca tattatacgg agcaggagag
    21001 ttcacatgtc tgctgttaaa aagcagcgta tcgatctgcg tttaactgac gatgacaaaa
    21061 gtatgattga ggaagcggca gcgatatcta atcagtccgt cagccaattt atgttgaaca
    21121 gcgcttcgca gcgggctgcg gaagtgattg aacaacatcg gcgggtgatc ctcaatgagg
    21181 aatcctggac gcgggtgatg gatgcgctga gtaatccacc gtcaccaggt gaaaagctaa
    21241 aacgtgcggc aaaacgtctt cagggaatgt aataatttat ggatgatctg acgatagaga
    21301 ttctgaccga tgatgcagat tatgatctac agcgattcga ctgcggcgag gaagcgttaa
    21361 atctctttct gacgacacat ctggttcgtc aacatcgcaa taaaattctg cgagcgtata
    21421 tcctttgtcg caacactcca gaacgtcagg tcatgggata ttacacatta tccggcagtt
    21481 gttttgaacg agtcgcacta ccctcgaaat cgaaacagaa aaaaattccc tacaaaaata
    21541 ttcccagcgt cactcttggg cgtctggcaa ttgatcgttc attacagggg cagggatggg
    21601 gagcaacact ggttgctcat gccatgaaag tcgtctggtc agcctcttta gcggtaggta
    21661 ttcacggtct ttttgtcgag gcgctgaatg aaaaagccca tacgttttat caatcgctgg
    21721 gctttatccc tttagtcggg gaaaacgaaa atgcgttatt tttcccaacc aaatccattg
    21781 aactgctttt tacacagagc gattaacgct tctcccagcc gccctgttgt gccgtaaacc
    21841 ccagcgcctg cataaacgcc gtcatcacac cgcgatcttc caagcctgca tccgccatcc
    21901 accagcatga aacgccagga ttgttacgca aaacctcttc cagcagatat tgccccacac
    21961 cgcgtcggcg ggtgacttcc cgtacgcgca aagaatccag aaccccctga gtgccgctta
    22021 aggttacccg cacggccgcg agcaggcgct cgttaaaacg cgcggcgtag atacggtggt
    22081 tatcgtcaac ctgtaaagtg gaaggggaat actccggcca gatcttttgc aggtcaatcc
    22141 ggtcctggtc gctaaagttt tctaatcgaa tgatggtcag cttcatgggg aacccgtgta
    22201 aatcacaaaa gtggaaccag tgtagcgaaa taatttaatc ggaggctttc tcttttttat
    22261 ttcttttggc aggtgattaa ttttttaaca gcaataatta caaagttaaa acattatata
    22321 atgaaaaata cccagcataa tcccctgaat gatagtgaat tattccgccc tttgtgccgt
    22381 tattttatgc tgacaacggc acttttttct gtttatctat caataaattc agaatattat
    22441 ctgttcttaa tcgactgaaa aatagggatt ttaatcgcta ttatcacaaa atactgcgct
    22501 aaacccttaa tcagacaggc aaaaacagtg cagtataaaa aaagaacagt ctgatttgtt
    22561 aacacataaa aacaaagcaa cacaacatca cgaatgggga tttttgacta tgaaacggaa
    22621 tgcgaaaact atcatcgcag ggatgattgc actggcaatt tcacacaccg ctatggctga
    22681 cgatattaaa gtcgccgttg tcggcgcgat gtccggcccg attgcccagt ggggcgatat
    22741 ggaatttaac ggcgcgcgtc aggcgattaa agacattaat gccaaagggg gaattaaggg
    22801 cgacaagctg gtgggcgtgg aatatgacga cgcctgcgac ccgaaacaag ccgttgcggt
    22861 cgccaacaaa atcgttaacg acggcattaa atacgttatt ggtcatctgt gttcttcttc
    22921 tacccagcct gcgtcagata tctatgaaga tgaaggtatt ctgatgatct cgccgggagc
    22981 aaccaacccg gagctgaccc aacgcggtta tcaacacatt atgcgtactg ccgggctgga
    23041 ctcttcccag gggccaacgg cggcaaaata cattcttgag acggtgaagc cccagcgcat
    23101 cgccatcatt cacgacaaac aacagtatgg cgaagggctg gcacgttcgg tgcaggacgg
    23161 gctgaaagcg gctaacgcca atgttgtttt cttcgacggc attaccgctg gcgagaaaga
    23221 tttctccgcg ctgatcgccc gcctgaaaaa agaaaacatc gacttcgttt actacggcgg
    23281 ttactacccg gaaatggggc agatgctgcg ccaggcccgt tccgttggcc tgaaaaccca
    23341 gtttatgggg ccggaaggtg tgggtaatgc gtcgttgtcg aatattgccg gtgatgccgc
    23401 cgaaggcatg ttggtcacta tgccaaaacg ctatgaccag gatccggcaa accagggcat
    23461 cgttgatgcg ctgaaagcag acaagaaaga tccgtccggg ccttatgtct ggatcaccta
    23521 cgcggcggtg caatctctgg caactgccct tgagcgtacc ggcagcgatg agccgctggc
    23581 gctggtgaaa gatttaaaag ctaacggtgc aaacaccgtg attgggccgc tgaactggga
    23641 tgaaaaaggc gatcttaagg gatttgattt tggtgtcttc cagtggcacg ccgacggttc
    23701 atccacggca gccaagtgat catcccaccg cccgtgaaat gcgggcgggt ttagaaaggt
    23761 taccttatgt ctgagcagtt tttgtatttc ttgcagcaga tgtttaacgg cgtcacgctg
    23821 ggcagtacct atgcgctgat agccatcggc tacaccatgg tttacggcat tatcggcatg
    23881 ataaacttcg cccacggcga ggtttatatg attggcagct acgtctcatt tatgatcatc
    23941 gccgcgctga tgatgatggg cattgatacc ggctggctgc tggtggcggc gggattcgtc
    24001 ggcgcaatcg tcattgccag cgcctacggc tggagtatcg aacgggtggc ctatcgcccg
    24061 gtgcgtaatt ctaagcgcct gattgcgctc atctcagcaa tcggtatgtc catcttcctg
    24121 caaaactacg tcagcctgac tgaaggttcg cgcgacgtgg cgctgccgag cctgtttaac
    24181 gggcagtggg tggtgggcca tagcgaaaac ttctctgcct ctattaccac catgcaggcg
    24241 gtgatctgga ttgtcacctt cctcgccatg ctggcgctga cgattttcat tcgctattcc
    24301 cgcatggggc gcgcctgtcg tgcctgcgcg gaagatctga aaatggcgag cctgcttggc
    24361 atcaacaccg accgggtgat tgcgctgacc tttgtgattg gcgcagcgat ggcggcggtg
    24421 gcaggtgtgc tgctcggtca gttctacggc gtcattaacc cctacatcgg ctttatggcc
    24481 gggatgaaag cctttaccgc tgcggtgctc ggtgggattg gcagcattcc gggagcgatg
    24541 attggcgggc tgattctggg gattgcggag gcgctctctt ctgcctatct gagtacggaa
    24601 tataaggatg tggtttcatt tgccctgctg attctggtgc tgctggtgat gccgaccggt
    24661 attctgggtc gcccggaggt agagaaagta tgaaaccgat gcatattgca atggcgttgc
    24721 tctctgccgc gatgtttttt gtcctggcgg gcgtctttat gggcgtacaa ctggagctgg
    24781 atggcaccaa actggtggtc gacacggctt cagacatccg ttggcagtgg gtgtttatcg
    24841 gcacggcggt ggtctttttc ttccagcttt tgcgaccggc tttccagaaa gggttgaaaa
    24901 gcgtttccgg accgaagttt attctgcccg ccattgatgg ctccacggtg aagcagaaac
    24961 tattcctcgt ggcgctgctg gtgcttgcgg tggcgtggcc gtttatggtg tcgcgtggga
    25021 cggtggatat cgccaccctg accatgatct acattatcct cggcctcggg ctgaacgtgg
    25081 ttgttggtct ttctggtctg ctggtgctgg ggtacggcgg cttttacgcc atcggcgctt
    25141 acacctttgc gctgctcaat cactattatg gtctgggctt ctggacctgt ctgccgattg
    25201 ccgggttaat ggcggcagcg gcgggcttcc tgctcggttt tccggtgctg cgtctacgcg
    25261 gtgactatct ggcgatcgtt accctcggtt tcggcgaaat tgtgcgcata ttgctgctca
    25321 ataacaccga aattaccggc ggcccgaacg gaatcagtca gatcccgaaa ccgactttct
    25381 tcggccttga gttcagccgt accgctcgtg aaggcggctg ggacacgttc agtaatttct
    25441 ttggcctgaa atacgatccc tccgatcgcg tcatcttcct gtatctggtg gcgttgctgc
    25501 tggtggtgct aagcctgttt gtcattaacc gcctgctgcg gatgccgctg gggcgtgcgt
    25561 gggaagcatt gcgtgaagat gaaattgcct gccgttcgct gggcttaagc ccgcgtcgta
    25621 tcaagctgac cgcctttacc atcagcgccg cgtttgccgg ttttgccgga acgctgtttg
    25681 ctgcgcgcca gggctttgtc agcccggaat ccttcacctt tgctgaatcg gcgtttgtac
    25741 tggcgatagt ggtgctcggc ggtatgggat cgcagtttgc ggtgattctg gcggcaattt
    25801 tgctggtggt atcgcgcgaa ttgatgcgtg atttcaacga atacagcatg ttaatgctcg
    25861 gtggtttgat ggtgctgatg atgatctggc gtccgcaggg cttgctgccc atgacgcgcc
    25921 cgcaactgaa gctgaaaaac ggcgcagcga aaggagagca ggcatgagtc agccattatt
    25981 atctgtgaac ggcctgatga tgcgcttcgg cggcctgctg gcggtgaaca acgtcaatct
    26041 tgaactgtac ccgcaggaga tcgtctcgtt aatcggccct aacggtgccg gaaaaaccac
    26101 ggtttttaac tgcctgaccg gtttctacaa acccaccggc ggcaccattt tactgcgcga
    26161 tcagcatctt gaaggtttac cggggcagca aattgcccgc atgggcgtgg tgcgcacctt
    26221 ccagcatgtg cgtctgttcc gtgaaatgac ggtaattgaa aacctgctgg tggcgcagca
    26281 tcaacaactg aaaaccgggc tgttctctgg cctgttgaaa acgccttctt tccgtcgcgc
    26341 ccagagcgaa gcgctcgacc gcgccgccac ctggcttgag cgcattggtt tgctggaaca
    26401 cgccaaccgt caggcgagta acctggccta tggtgaccaa cgccgtctgg agattgcccg
    26461 ctgcatggtg acgcagccgg agattttaat gctcgacgaa ccggcggcag gtcttaaccc
    26521 aaaagaaacc aaagagctgg atgagctgat tgccgaactg cgcaatcatc acaacaccac
    26581 tatcttgttg attgaacacg atatgaagct ggtgatgggg atttcagacc gaatttatgt
    26641 ggtcaatcag gggacgccac tggcaaacgg tacgccggag caaatccgta ataacccgga
    26701 cgtgatccgt gcctatttag gtgaggcata agatggaaaa agtcatgttg tcctttgaca
    26761 aagtcagcgc ccactacggc aaaatccagg cgctgcatga ggtgagcctg catatcaatc
    26821 agggcgagat tgtcaccctc attggcgcga acggggcggg gaaaaccaca ttgctcggca
    26881 cgttatgcgg cgatccgcgc gctaccagcg ggcgaattgt gtttgatgat aaagacatta
    26941 ccgactggca gacggcgaaa atcatgcgcg aagcggtagc gattgtcccg gaagggcgtc
    27001 gcgtcttctc gcggatgacg gtggaagaga acctggcgat gggcggcttt tttgccgaac
    27061 gcgaccagtt ccaggagcgc ataaagtggg tgtatgaact gtttccacgt ctgcatgagc
    27121 gccgtgttca gcgggcaggc accatgtccg gcggtgagca acaaatgctg gcgattggtc
    27181 gcgcgctgat gagcaacccg cgtttgctgc tacttgatga gccatcgctc ggtcttgcgc
    27241 cgattatcat ccagcaaatt ttcgacacca tcgagcagct gcgcgagcag gggatgacta
    27301 tcttcctcgt cgagcagaac gccaaccagg cgctaaagct ggcggatcgc ggctacgtgc
    27361 tggaaaacgg ccatgtggtg ctctccgata ccggcgacgc gctgctggcg aacgaagcag
    27421 tgagaagtgc gtatttaggc gggtaataac acgttgattg ataaggagtc aaaagactcc
    27481 tttgagacag gtgacaaatg taaaattgcc tgatgcgctg cgcttatcag gcctactggg
    27541 tgagttgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt ttacgccgca
    27601 tccggcatga aacgatgagt aatctgtaga gtttgattca gaccttctat tttccgctta
    27661 tccgtgcccc atctcccatt ttccctcacc cacgccgtca ccgccttgtc atctttctga
    27721 caccttacta tcttacaaat gtaacaaaaa agttattttt ctgtaattcg agcatgtcat
    27781 gttaccccgc gagcataaaa cgcgtgaatt cgcgcattcg gtacaacaag agagataaac
    27841 gatgaaaccg ttacattata cagcttcagc actggcgctc ggactggcgt taatggggaa
    27901 tgcacaggca gtgacgacca ttccgttctg gcattctatg gaaggggaac tgggtaaaga
    27961 ggtggattct ctggcccaac gttttaacgc cgaaaatccg gattacaaaa ttgtaccgac
    28021 ctataaaggc aactacgaac agaatttaag cgcggggatt gccgcatttc gtaccggcaa
    28081 cgctccggct attttgcagg tttatgaagt tggcaccgcc accatgatgg catcgaaagc
    28141 cattaaaccg gtatatgacg tgtttaaaga ggcggggatt caattcgatg agtcgcagtt
    28201 tgtgccgacg gtttccggtt actactccga cagcaaaacg ggccacttac tctcccagcc
    28261 attcaacagc tcgacccccg ttctctatta caacaaagac gccttcaaga aagcaggatt
    28321 agacccggaa caaccgccga aaacctggca ggatctggcg gactatgccg cgaaactgaa
    28381 agcctccggt atgaagtgcg gctacgccag cggctggcag ggctggatcc aactggaaaa
    28441 ctttagcgcc tggaacggtc tgccgtttgc cagcaaaaac aacggctttg acggcacgga
    28501 cgcggtgctg gagttcaaca agccggagca ggtgaaacac atcgccatgc tcgaggagat
    28561 gaacaagaag ggcgacttca gctacgtcgg tcgtaaggat gaatccaccg agaagttcta
    28621 taacggtgat tgcgcgatga ccaccgcctc ttccggttct ctcgctaata ttcgtgagta
    28681 cgccaaattt aactatggcg taggcatgat gccttacgac gccgatgcga aagatgcacc
    28741 gcaaaacgcc attatcggcg gggccagcct gtgggtgatg cagggcaaaa ataaagaaac
    28801 gtataccggc gtggcgaagt tcctcgactt cctcgcgaag ccagaaaacg ctgccgagtg
    28861 gcatcagaaa accggttatc tgccaatcac caaagcagcg tatgacctga cccgtgagca
    28921 gggcttttat gagaaaaacc caggggcgga taccgcgacg cgtcagatgc tgaacaagcc
    28981 gccgttgccg ttcaccaaag ggctgcgtct gggcaacatg ccgcagatcc gcgtgattgt
    29041 ggatgaagag ctggagagcg tgtggaccgg taagaagaca ccacagcagg cactggatac
    29101 cgccgttgag cgtggaaatc agttgctgcg ccgctttgag aaatcgacga agtcttaatc
    29161 agtgtaatgt cggatgcgtt tcgcttatct gacctggcat cgcgtgtagg ccggataagc
    29221 gaagcgcatc cggcacagtt caggaattaa ccgtaatgtc atcatcccgt ccggtgttcc
    29281 gctcgcgctg gctgccttat ctgctggtcg cgccgcagct catcatcacc gttatctttt
    29341 ttatctggcc tgcgggcgaa gcgttgtggt actcgctaca aagcgtcgat ccgtttggtt
    29401 tctccagcca gtttgtcggc ctggataact tcgtcgcgct gtttcatgac agctactatc
    29461 tcgactcctt ctggacgacg ataaaattca gcacctttgt caccgtcagc ggtttgctgg
    29521 tgtcgctgtt cttcgcggcg ctggtggagt acatcgtgcg cggcagccgt ttctatcaaa
    29581 ccttaatgtt gctgccttac gccgtggctc ccgccgttgc cgccgtattg tggatcttcc
    29641 tgtttaaccc cggtcgcggg ctgatcaccc attttctcgc ggagttcggc tacgactgga
    29701 accacgcgca aaacagcggt caggcaatgt ttctggtggt gtttgcctca gtatggaagc
    29761 aaatcagcta caacttcctg ttcttctatg ccgcgctgca atccattccc cgttcgttga
    29821 tcgaagccgc agccatcgac ggtgcagggc cgattcgccg cttctttaag attgcgctgc
    29881 cgcttatcgc cccggtgagt ttcttcctgc tggtagtgaa cctggtgtat gccttcttcg
    29941 acaccttccc ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc
    30001 tgatttataa gatctaccgc gaaggtttta ccggactgga tctggcttcg tctgccgcac
    30061 agtcggtggt gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg
    30121 aaagcaaggt gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac
    30181 catgctgatc ctcgggatcg cggtgatcct cttcccgctg tacgtggcgt ttgtcgcggc
    30241 gacgctggat aaacaggccg tctatgccgc gccgatgacg ctcatccccg gcacacatct
    30301 gctggaaaac atccacaaca tctgggtgaa cggggtaggc acgaatagcg cgccgttctg
    30361 gcggatgttg cttaacagct ttgtgatggc gttcagcatt acgctcggca aaattaccgt
    30421 ctcgatgctc tcggcatttg ccattgtctg gtttcgtttt ccgctacgta acctcttctt
    30481 ctggatgatt tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga
    30541 agtcatcgcc aacctgcaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc
    30601 ctcggcgacc gctactttcc tgttccgcca gttctttatg acgctgccgg atgagctggt
    30661 ggaagccgcg cggatcgacg gcgcatcgcc aatgcgcttc ttttgcgaca tcgtttttcc
    30721 gctctccaaa actaatctgg cggcgctgtt tgtgatcacc tttatctacg gctggaatca
    30781 gtatttgtgg ccgttgttga ttattaccga cgttgatctc ggcaccaccg tggcagggat
    30841 caaagggatg atcgccactg gcgaaggcac cacggaatgg aactcggtaa tggcggcgat
    30901 gttgttaacg cttatccctc cggtggtgat tgttttagtg atgcagcgtg ccttcgtgcg
    30961 cggcctggtc gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa
    31021 gctgggatgg caaaacccag gtgattaaac cactgaccct tgatgtggcg gatggcgaat
    31081 ttatcgtgat ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgc atggttgccg
    31141 ggctggagcg ggtgacagaa ggcgatatct ggatcaacga ccagcgcgtg actgaaatgg
    31201 agccaaaaga tcgcgggatt gcgatggtgt tccagaatta cgcgctttat ccgcatatga
    31261 gcgtagagga gaacatggcg tgggggctga aaattcgcgg catgggcaag cagcaaattg
    31321 ccgagcgcgt taaagaagcg gcgcgcattc tggaactgga cggtctgctt aagcgtcgcc
    31381 cgcgtgagct atccggcggt cagcgccagc gtgtggcgat gggccgcgcg attgtgcgcg
    31441 atccggcggt gttcctgttt gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc
    31501 agatgcgtct tgaactgcaa cagctgcacc gtcgcctgaa aacgacttca ctctacgtta
    31561 ctcacgatca ggttgaagcg atgacgctcg ctcagcgagt aatggtgatg aacggcggcg
    31621 ttgccgaaca gattggcaca ccagttgaag tctacgaaaa gcccgccagc ctgtttgtgg
    31681 cgagttttat cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat aatgaaggca
    31741 cgcatttcga actggacggc ggtatcgtgc taccgctaaa cggtggctac cgtcagtatg
    31801 ccgggcgtaa aatgactctc ggcattcgcc cggaacatat agcgctaagc tcgcaggcag
    31861 aaggtggtgt gccgctggtg atggacacgc tggagatcct cggcgcagat aacctggcgc
    31921 acggacgctg gggcgagcag aagctggtgg tgcgactggc gcatcaggag cgcccgacgg
    31981 caggcagcac gctgtggctg catctggcgg aaaatcagct gcatcttttt gatggtgaaa
    32041 caggacaacg agtatgagta actggcctta tccccgcatc gtcgctcatc gtggcggcgg
    32101 taagctggcc ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat acggtcataa
    32161 gatgatcgaa tttgacgcga agttatcgaa agatggcgag atcttcctgc tccatgacga
    32221 caatctcgaa cgcaccagca acggctgggg cgtcgcgggt gaactgaact ggcaggattt
    32281 actgcgcgtg gatgcgggca gttggtacag caaagcgttt aaaggtgagc cgctgccgtt
    32341 gctttcgcag gtggcggaac gctgtcgcga acacgggatg atggcgaata tcgaaatcaa
    32401 acccaccact ggcactggac cattaacggg caaaatggtg gcgctggcgg cacgccaact
    32461 gtgggccggt atgacgccgc cgctgctgtc atcgtttgag attgatgctt tagaagccgc
    32521 acaattggcg gcaccggaac tgccgcgtgg tttattgctg gatgagtggc gcgacgactg
    32581 gcgcgaactg accatgcggc tgggctgcgt ctctcttcat ctcaatcata agttgctcga
    32641 taaagcgcga gtgatgcagt tgaaagacgc cggactgcgg attctggtgt ataccgtcaa
    32701 caaaccccag cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtaccgatgc
    32761 gattgatgta attggtccga actttacggc ccaatagttt tcaacggaat gtcaggctgc
    32821 ggcgtgctgg gctgattcag catgtcgccg tttctcctct ccggcagcat atgctgctga
    32881 ttcaacgaac tatccggatt acggttgctg tttaacatcc cgccgttggt gttgggcagc
    32941 atttgctgcc gggcgggatt tcgctccccc ggctgtgact gcaacacccg ctgagaatta
    33001 ttgtttatct ggttttctaa atgctgctgt tgcaactgag tttgcgtttt cagttgctga
    33061 ttcagcatcc ctttttgctg gatttgctgc gtttgcattt gggtttgcat ccgctgctga
    33121 ctggggatct gataccccgg ctggttaggg ttattcagag tattaatggg ctgtgcaaag
    33181 ccggcaaacg gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg
    33241 aagatattct ttaagtttac tcgcttcccg gcaaaacgat gattaattca gagttatata
    33301 ccaggcttag ctggggttgc cccacacata actggagaat aacgatgata aaaccgacgt
    33361 ttttacgccg ggtggccatt gctgctctgc tctcaggaag ttgttttagc gccgccgccg
    33421 cgcctcctgt ttcttatggc gtggaggaag atgtctttca tccggtacgc gcgaaacagg
    33481 ggatggtggc gtctgtagat gctactgcca ctcaggtggg ggtggatatc ctcaaggagg
    33541 gcgggaatgc cgttgatgct gccgtggcgg tgggctacgc gctggcggta acgcatccgc
    33601 aggcggggaa tctgggcggc ggtggtttta tgctgatccg cactaaagat ggcaacacca
    33661 ctgctattga tttccgtgag atggcacccg ctaaagcgac ccgcgatatg ttccttgatg
    33721 atcaaggcaa cccggacagc aaaaaatcac tcacttcgca tctggcctct ggcacgcccg
    33781 gtacggtagc aggtttctcg ctggcgttgg ataaatacgg caccatgccg ctgaataaag
    33841 tcgtgcagcc cgcttttaaa cttgcacgcg atggttttat cgttaacgac gcgctggctg
    33901 acgatctcaa aacctacggt agcgaagtgt tgccgaatca cgaaaacagc aaagctatct
    33961 tctggaaaga gggcgaaccg ctgaaaaagg gcgacaagct ggtgcaggcg aatctggcaa
    34021 agagcctgga gatgattgcc gaaaacggcc cggacgaatt ctacaaaggc acgatagcgg
    34081 aacagattgc ccaggagatg cagaaaaacg gtggcttgat caccaaagaa gatttagcgg
    34141 cgtataaagc ggtcgaacgc acaccgataa gcggcgatta tcgcgggtat caggtttact
    34201 ccatgccacc gccatcttcc ggcgggatcc atatcgtaca aatcctcaat attttggaaa
    34261 acttcgatat gaagaaatac ggctttggca gcgccgatgc gatgcaaatc atggcagaag
    34321 cggagaaata tgcctacgcc gaccgctcgg aatatcttgg cgacccggat tttgtcaaag
    34381 tcccgtggca ggcactgacc aataaagcct atgccaaatc cattgccgat caaattgata
    34441 tcaacaaagc gaagccgtcc agcgaaatcc gtcccggcaa gcttgcgcct tatgagagta
    34501 atcaaactac ccattattcg gtggtggata aagacggtaa cgcggtggcg gtgacctata
    34561 cgctgaacac caccttcggt acgggtattg tcgcgggcga gagcggtatt ctgcttaata
    34621 accagatgga tgatttctcc gccaaaccgg gcgtgccgaa cgtttacggg ctggtgggcg
    34681 gtgatgccaa cgccgtcggg ccgaataaac gtccactgtc gtcgatgtcg ccgactattg
    34741 tagtgaaaga cggtaaaacc tggctggtta ccggaagccc aggtgggagt cggattatca
    34801 ccactgtgct gcaaatggtg gtgaacagca tcgattatgg catgaacgtc gccgaagcga
    34861 ccaatgcacc gcgtttccac catcagtggt tgccggacga gctgcgtgtc gaaaaagggt
    34921 ttagcccgga tacgctcaag ctgctggaag caaaaggtca gaaagtggcg ctgaaagagg
    34981 cgatgggcag cacacaaagt attatggttg ggcctgacgg tgagttgtac ggtgcatctg
    35041 acccgcgctc ggtggatgat ttaacggcgg ggtactaagg ttagcggccc tcttcgtggg
    35101 aagagggcta ttttgtcagg gcaagccgaa ggtagccttt tttatttcgt aatcctgtag
    35161 atattcttcc agcggctttg ccgttcccag agaatgaatt tcaccactgt ctttatcaat
    35221 aataaaaggt gcgttaccag ctaagcgcgc ggcctcatct ccagtttcga gaaattctcg
    35281 tgcttcgaaa cagaaatacc agccctggct aaagcgtcca tgtagagtaa tgacgaccgg
    35341 gagatctgca tcatcaaggt aatggttcgc tttcgcgaat gcgtcgtgat aagtaatcat
    35401 aattataata aat
//
LOCUS       DAZGXZ010000041        35087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000041 DAZGXZ010000000
VERSION     DAZGXZ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.041"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            88..672
                     /gene="yadN"
                     /locus_tag="ACHKJF_002097"
     CDS             88..672
                     /gene="yadN"
                     /locus_tag="ACHKJF_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0949456.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            777..1517
                     /gene="yadV"
                     /locus_tag="ACHKJF_002098"
     CDS             777..1517
                     /gene="yadV"
                     /locus_tag="ACHKJF_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0949457.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1552..4152
                     /gene="htrE"
                     /locus_tag="ACHKJF_002099"
     CDS             1552..4152
                     /gene="htrE"
                     /locus_tag="ACHKJF_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFP0949458.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGHCLNLTEIIPQASVRYDVNDQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAVMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYVQRDIASLRSQLIIGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQILKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQLTDNNYTAGLLGLGLNTSVGAFSFDVTHSNVSIPDDKTYEGQSYRVSWN
                     KLFENTKTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKNMRNYSRMKNQVT
                     VSINQPLKLEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSISAQRSWN
                     EDGDTDDSVYLSFTVPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNISSSGYSDNA
                     RVSYSVNTGYTMNKASEDLSYVGGYASYESPWGTLAGSVSANSDNSSQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFAGFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIDQQGNISIKWIEESKPVSCLAHYQQSSEAEKLAQSI
                     ILNGIRCQIQ"
     gene            4169..4744
                     /gene="yadM"
                     /locus_tag="ACHKJF_002100"
     CDS             4169..4744
                     /gene="yadM"
                     /locus_tag="ACHKJF_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0949459.1"
                     /translation="MIKTTPHKIAILMGILLSPSVFATTDINVEFTATVKATTCNITL
                     TGTNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVARECSTGISWIDTTLTGNS
                     SSSSPKLLIPQSGDSSSTTSYIGMGFKKRTTDDATFLQPNNSAEKIRWSTNEMQPDKG
                     LEMTVALRETEAGKGVPGNFRALATFNFIYQ"
     gene            4759..5361
                     /gene="yadL"
                     /locus_tag="ACHKJF_002101"
     CDS             4759..5361
                     /gene="yadL"
                     /locus_tag="ACHKJF_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP0949460.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEMGT
                     CTATLVNDSDQNISVVDFDDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTVTPATGSATQFSCVTPASQEVTISTA
                     ANEVVYYPMSARLVVEKNKTVNNVTAGKFSAPATFTVTYN"
     gene            5388..5984
                     /locus_tag="ACHKJF_002102"
     CDS             5388..5984
                     /locus_tag="ACHKJF_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0949461.1"
                     /translation="MHPTQRKLMKRIILFLSLLFPITCPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSGNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPANIFNVISSAGNSRSVYPVMTS
                     ALNNSSWKFYARMQKIDPALDVISGQVISHILVDVYYE"
     gene            5998..7311
                     /locus_tag="ACHKJF_002103"
     CDS             5998..7311
                     /locus_tag="ACHKJF_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFP0949462.1"
                     /translation="MTATVTAVDGGVIMKKFFSHFLFLILCLNCYTASAGTDDNVGYI
                     VGNSYGVGPSDQKWRETGPNGDATVVFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDT
                     ASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSA
                     YGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIE
                     FYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPT
                     CFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVG
                     TENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTT
                     GGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7424..8218
                     /gene="panB"
                     /locus_tag="ACHKJF_002104"
     CDS             7424..8218
                     /gene="panB"
                     /locus_tag="ACHKJF_002104"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP0949463.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8230..9081
                     /gene="panC"
                     /locus_tag="ACHKJF_002105"
     CDS             8230..9081
                     /gene="panC"
                     /locus_tag="ACHKJF_002105"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP0949464.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSENSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(9155..10057)
                     /gene="rpnC"
                     /locus_tag="ACHKJF_002106"
     CDS             complement(9155..10057)
                     /gene="rpnC"
                     /locus_tag="ACHKJF_002106"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP0949465.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            10331..10711
                     /gene="panD"
                     /locus_tag="ACHKJF_002107"
     CDS             10331..10711
                     /gene="panD"
                     /locus_tag="ACHKJF_002107"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP0949466.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10715..11944)
                     /gene="yadE"
                     /locus_tag="ACHKJF_002108"
     CDS             complement(10715..11944)
                     /gene="yadE"
                     /locus_tag="ACHKJF_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0949467.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12008..12448)
                     /gene="yadI"
                     /locus_tag="ACHKJF_002109"
     CDS             complement(12008..12448)
                     /gene="yadI"
                     /locus_tag="ACHKJF_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0949468.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12553..13323)
                     /gene="yadH"
                     /locus_tag="ACHKJF_002110"
     CDS             complement(12553..13323)
                     /gene="yadH"
                     /locus_tag="ACHKJF_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0949469.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13320..14246)
                     /gene="yadG"
                     /locus_tag="ACHKJF_002111"
     CDS             complement(13320..14246)
                     /gene="yadG"
                     /locus_tag="ACHKJF_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0949470.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14355..15017
                     /gene="can"
                     /locus_tag="ACHKJF_002112"
     CDS             14355..15017
                     /gene="can"
                     /locus_tag="ACHKJF_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP0949471.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15058..15594)
                     /gene="hpt"
                     /locus_tag="ACHKJF_002113"
     CDS             complement(15058..15594)
                     /gene="hpt"
                     /locus_tag="ACHKJF_002113"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP0949472.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYICKVILLDE"
     gene            15800..18190
                     /gene="gcd"
                     /locus_tag="ACHKJF_002114"
     CDS             15800..18190
                     /gene="gcd"
                     /locus_tag="ACHKJF_002114"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP0949473.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMRVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            18229..18327
                     /locus_tag="ACHKJF_002115"
                     /pseudo
     CDS             18229..18327
                     /locus_tag="ACHKJF_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18392..19942)
                     /gene="cueO"
                     /locus_tag="ACHKJF_002116"
     CDS             complement(18392..19942)
                     /gene="cueO"
                     /locus_tag="ACHKJF_002116"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP0949474.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQHGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20108..20455
                     /gene="yacC"
                     /locus_tag="ACHKJF_002117"
     CDS             20108..20455
                     /gene="yacC"
                     /locus_tag="ACHKJF_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP0949475.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20561..21427
                     /gene="speE"
                     /locus_tag="ACHKJF_002118"
     CDS             20561..21427
                     /gene="speE"
                     /locus_tag="ACHKJF_002118"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP0949476.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLTHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21443..22237
                     /gene="speD"
                     /locus_tag="ACHKJF_002119"
     CDS             21443..22237
                     /gene="speD"
                     /locus_tag="ACHKJF_002119"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP0949477.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22275..22637)
                     /gene="yacL"
                     /locus_tag="ACHKJF_002120"
     CDS             complement(22275..22637)
                     /gene="yacL"
                     /locus_tag="ACHKJF_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP0949478.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22813..25410)
                     /gene="acnB"
                     /locus_tag="ACHKJF_002121"
     CDS             complement(22813..25410)
                     /gene="acnB"
                     /locus_tag="ACHKJF_002121"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP0949479.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25765..27618
                     /gene="yacH"
                     /locus_tag="ACHKJF_002122"
     CDS             25765..27618
                     /gene="yacH"
                     /locus_tag="ACHKJF_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP0949480.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(27689..29113)
                     /gene="lpdA"
                     /locus_tag="ACHKJF_002123"
     CDS             complement(27689..29113)
                     /gene="lpdA"
                     /locus_tag="ACHKJF_002123"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP0949481.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29438..31330)
                     /gene="aceF"
                     /locus_tag="ACHKJF_002124"
     CDS             complement(29438..31330)
                     /gene="aceF"
                     /locus_tag="ACHKJF_002124"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP0949482.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGSDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31345..34008)
                     /gene="aceE"
                     /locus_tag="ACHKJF_002125"
     CDS             complement(31345..34008)
                     /gene="aceE"
                     /locus_tag="ACHKJF_002125"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP0949483.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34169..34933)
                     /gene="pdhR"
                     /locus_tag="ACHKJF_002126"
     CDS             complement(34169..34933)
                     /gene="pdhR"
                     /locus_tag="ACHKJF_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP0949484.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8798 a         9096 c         8455 g         8738 t
ORIGIN      
        1 tctagcatct gcttataaca tttgtgtcat gaatagtcat tcttcaaacc agtagtctga
       61 ctaatggaat tttattcaag gatttttatg tctaaaaaat taggttttgc cctgtctggt
      121 cttatgctgg caatggttgc aggtactgct tctgctgata tggatggtgg tcagttaaat
      181 atcagtggtc tggttgttga taacacctgt gaaacccgcg ttgacggcgg taataaagat
      241 ggtctgatcc tgctgcaaac tgcaactgtt ggtgaaatcg atgctggcgt gctgaatgac
      301 accgttggcg ctaaagccaa acctttcagc atcaccgttg actgcagcaa agcaaatccg
      361 aatccaggta gtacagctaa aatgaccttc ggttctgttt tctttggtaa tagcaaagga
      421 acgctgaaca acgacatgtc tattaacaac ccgtctgacg gcgtgaatat cgctctgcac
      481 aatattgatg gttccactat caaacaggtt caaattaaca accctggcga tgtgtatact
      541 aaagccctgg atgcaacgac aaaatctgct gtttatgatt ttaaagcgtc ttacgttcgt
      601 gccgtcgcag accaaacagc aactgctggt tatgtaaaaa ctaacactgc atacaccatt
      661 acttatcagt aagtattcac caattaaatt gattgaatga atatacaggg aatagtgtta
      721 ataatttcta tttttatatt attccctgtt ttaattaact ctgtcaggga tggtttatgt
      781 tttttaacac caaacataca acagctttat gcttcgtaac ctgtatggct tttagttcat
      841 cgtctattgc ggacattgtc atttcgggta ctcgcgtaat atataaaagc gatcaaaaaa
      901 gtgtcaacgt acgtctggaa aataaaggga ataacccgtt gcttgtccag agttggttag
      961 atactggcga tgacaacgct gagcctggca gtattacagt cccttttact gccacgccgc
     1021 cagtatcgcg tattgatgcc aaacgtgggc aaacaatcaa attaatgtac acagccagca
     1081 cctcactgcc taaagacaga gagagcgtgt tctggtttaa cgtactggaa gttccaccaa
     1141 aaccagatgc agaaaaggtc gcgaatcaaa gtctgctgca actggcattt cgcacacgta
     1201 taaaactttt ctatcgcccg gatggattga agggaaatcc ctctgaagcc ccgttagccc
     1261 tgaagtggtt ctggtcaggt tcagaaggta aggcgtcatt gcgagtgacc aatccaaccc
     1321 cttactacgt ctcttttagc agtggtgatt tagaagctag cggtaaacgc tatccgattg
     1381 atgtgaaaat gattgcacca tttagtgatg aggtcatgaa agtcactggc cttaatggca
     1441 aagcaagttc tgcaaaagta catttttacg ccattaatga ttttggtggc gccattgaag
     1501 gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa cgtgactata
     1561 aaaccaacta aaaattatca taatcatctg acccgtatcg ctacgttttg cgcgctgctg
     1621 tattgcaata gtgctttctg tgctgaactt gttgaatatg accatacctt cctgatgggg
     1681 cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccctgc tataccgggt
     1741 atgtacgacg tcagtgttta tgtcaacgat caacctatta ttaaccaaag cattacattt
     1801 attgaaattg aaggaaaaaa aaacgcacaa gcctgtatca cacttaagaa tttattgcag
     1861 tttcatataa attctcccga tataaataac gaaaaagccg ttctgcttgc cagggatgaa
     1921 acactcggcc attgccttaa tttgacggaa attatccctc aggcttctgt tcgttatgac
     1981 gttaacgatc aacgtctgga tatagacgtt cctcaggcct gggtaatgaa aaattaccaa
     2041 aactatgttg atccatcgtt atgggaaaat ggcattaatg cggtcatgtt gtcatacaac
     2101 ctcaacggat atcatagtga aactcctggt cgaagaaatg acagcattta tgctgctttt
     2161 aacggtggga tgaatttagg tgcatggcga ctgcgtgcct caggcaacta caactggatg
     2221 accgattctg gcagtaatta tgattttaag aatcggtatg tccagcgtga tatcgcctcg
     2281 ctgcgttctc aactcattat tggtgagtct tatacaacgg gcgaaacctt tgattctgtc
     2341 agtatccgcg gcattcgctt atacagtgac agccgcatgt tacctccgac attagccagt
     2401 tttgcgccta tcattcatgg tgttgccaat accaacgcca aagtcactat tacgcaaggt
     2461 ggctataaga tttatgaaac gactgtgccg ccaggcgctt tcgtcattga tgatctgagt
     2521 ccgtcaggtt acggcagcga tcttattgtt accgtcgaag agtcggatgg ctcaaagcgg
     2581 acattctcgc aacctttttc atcggttgtt caaatgttac gcccaggcgt tggacgttgg
     2641 gatattagcg gcggtcagat tttaaaagat gatattcagg atgagcctaa tttatttcag
     2701 gccagctact actatggttt gaataactac ctgacgggtt ataccggtat tcagcttacc
     2761 gataataatt ataccgccgg gttgttaggt cttggtctga atacctcagt cggtgcgttt
     2821 tctttcgatg taactcactc caatgttagt atcccggatg ataaaacgta cgagggtcaa
     2881 agttatcgcg tttcctggaa caagttattt gaaaatacca aaacctcgct caatatcgcg
     2941 gcttatcgtt attcaacgca gaattatctg gggcttaacg atgccctgac actgatcgac
     3001 gaagtaaaac atccggaaca ggacctggaa cctaaaaata tgcggaacta ttcacgcatg
     3061 aaaaatcagg ttacggtcag tattaaccaa ccgttgaaat tagagaaaaa agattacggt
     3121 tctttttatc tttctggtag ttggtctgat tactgggctt ccggacaaaa tcgtagcaat
     3181 tactccattg gctatagtaa cagtgcatcc tggggcagtt acagcatcag tgcacagcgt
     3241 tcatggaatg aagacggcga cactgacgat agcgtttatc tcagtttcac cgttcctatt
     3301 gaaaaattac ttggcactga acaacgtact tcaggttttc agagtattga tactcaaatg
     3361 agcagtgact ttaaggggaa taaccaactc aacatcagta gcagtggcta cagcgataac
     3421 gctcgtgtca gttatagcgt aaatactggc tatacgatga ataaagccag cgaagatttg
     3481 agttatgttg ggggttatgc cagctatgaa tcaccgtggg gaaccctggc aggttcagtg
     3541 tctgcaaata gcgataacag cagtcaagtt tctctcagca ccgacggtgg ttttgtatta
     3601 catagcggcg gcctgacctt cagcaatgac agttttagcg actccgatac actggcggta
     3661 gttcaggctc caggtgctca aggagcgcga ataaattatg gcaacagtac tatcgatcga
     3721 tggggttatg gtgttaccag tgctctatct ccttatcatg aaaaccgcat tgcgctggat
     3781 attaacgatc ttgagaacga tgttgaatta aaaagtacca gtgctgtagc tgtgccgcgt
     3841 cagggttcag tcgtctttgc tggttttgaa accgtgcaag ggcaatcagc cattatgaac
     3901 atcacacgaa gtgatggtaa aaatattcca tttgctgcag atatttatga tgagcaaggc
     3961 aatgtcattg gtaatgttgg acagggtgga caggcttttg ttcgtggtat tgatcagcag
     4021 ggaaatatca gcattaaatg gatagaagaa agcaaacccg taagttgcct tgcgcattat
     4081 caacaaagtt cagaggcaga aaaattagcc caatctatta ttctgaatgg aattcggtgt
     4141 cagattcagt aactacaagg gacgtcaaat gataaaaaca acgccacata aaatagcgat
     4201 actgatggga atattattat caccctcagt atttgcaacg acggatatta atgttgagtt
     4261 cacggccacc gtcaaagcga caacctgtaa catcaccctt actggtacta acgtcacgaa
     4321 tgatggcaat aataactaca cattgagaat ccctaagatg gggctggata agatcgcgaa
     4381 taaaacgaca gaatctcagg ctgattttaa actggttgcc agagagtgca gcactggcat
     4441 cagttggata gataccactc tgaccggaaa ctcatcatca agctcaccta agcttcttat
     4501 accgcagtct ggtgattcat catcgacgac aagttatatc ggtatgggtt tcaaaaaacg
     4561 gactactgat gatgcaactt tccttcaacc taacaacagt gcggaaaaga tccgctggag
     4621 cacaaacgaa atgcagcccg ataaaggtct tgaaatgacc gttgcgctac gtgaaacaga
     4681 ggctgggaaa ggtgtacctg gaaattttcg tgcgctagcg acgtttaatt tcatctatca
     4741 ataaaaagga taattaagat gacatttaaa aatttacgtt atggattatc cagcagtgtc
     4801 gttttggctg cctcattgtt cagcgtaccc tcttatgccg ctactgacag catcgggctg
     4861 accgttatta ccaccgtaga aatgggaact tgtacggcta cattggtaaa tgactctgat
     4921 cagaacattt ctgttgttga ttttgatgat gtatatattt ctgaaatcaa tgccaagacc
     4981 aaagtaaaaa cattcaaact caaattcaaa gactgtgcgg gtatccccaa taaaaaagcg
     5041 caaataaaat taaccaagcg agccacatgc gaaggaacgg ctaatgacgg tgcggggttt
     5101 gcaaatggtt ccacagccgc agataaagca agtgccgtcg ccgttgaagt ctggagcact
     5161 gtaactccgg caacaggaag tgcaacacaa tttagttgtg tgacaccagc atctcaagag
     5221 gtaacaatct ccactgcagc caacgaggtg gtttattatc cgatgagtgc acgcctggtc
     5281 gtcgaaaaaa ataaaaccgt aaacaatgtc acggcgggta aattttctgc accagccaca
     5341 tttacagtaa cctataacta aaagacttac agattcagga actatatatg catcccactc
     5401 aacgtaagct gatgaagaga ataattctgt ttctgtcact actgtttccc attacctgtc
     5461 cagccatcgc tgggcaggat attgaccttg ttgccaatgt aaaaaacagc acttgcaaaa
     5521 gcggaatcag taaccagggt aatattgatc ttggcgtcgt tggggtggga tatttttccg
     5581 gtaatgttac tcctgaaagc tatcaaccag gtggaaaaga gttcactgtc acagtatcag
     5641 actgtgcatt acagggaact ggcgatgtgc taaatcagtt acatattgat tttagagccc
     5701 ttagcggtgt catggctgca ggttcaaggc aaatatttgc caatgaagtc gcaaccgggg
     5761 ctaaaaatgt cggtgtcgtt ctcttttcca ttcaggaccc ggccaatata ttcaatgtta
     5821 ttagttcagc gggaaattct cgttccgtgt atccagtaat gacttctgcc ctgaataatt
     5881 cttcatggaa attttatgcc agaatgcaaa aaatcgatcc cgcgctggat gtcatatctg
     5941 gccaggtcat aagtcatata ttagttgatg tgtactacga ataatagatc aaaacaaatg
     6001 actgcgactg ttacagcagt agatggtgga gtgataatga agaaattctt cagccatttt
     6061 ctttttttaa ttctgtgctt gaattgttat accgcgagtg ctggcactga tgataacgta
     6121 ggatatattg ttgggaatag ttacggcgta ggtccgtcag atcaaaaatg gagagaaacg
     6181 gggccgaatg gcgatgctac tgtcgtattc agatatgcaa caagtacaaa taatttagtt
     6241 ttctacaaac cgacgcagct tggacctaca ggtgtaaaat tacagtggag tcagttagat
     6301 accgcttctg gtggtggttt tctttattgc aacagaagtg atagcacaag tggtagcgca
     6361 atgcgtattg aaaatgcaat ggttgactca ggtaaaatgt atggctccca taaattattt
     6421 aatacatcag ttcctggttt gtattacaca ttattaattt caaacatgtg gtcagcttac
     6481 ggtaccgtaa ctaacgttag ttctcctggg atatacattg gtgactctgc agaacaatat
     6541 ttttcgtggt ataatccaag cgaagatgtg ttatattgga gttgcaataa tgcgaatagc
     6601 acccgtaaat actgggctgt aggtggtatt tatcagaccc ttacaattga attctataca
     6661 gatacaaact ttgatccaac agtaactcaa cagatcaaat tatccagctc atcaaattat
     6721 ctgtattcat ttaaagccta tggcgcaggc caaggtataa atgagcatag ttattttatc
     6781 aaaatcgatt tcgatctgct caatgtcaaa ttgactaatc ccacttgttt taccgctatg
     6841 cttagtggaa cttctgtgac tggttctacg gtaaaaatgg gtgaatatag tgcagaacaa
     6901 atcagaaacg gtgccacacc ggttcctttt gatatttcac ttcaaaactg cgttcgtgtg
     6961 actaatattg agacaaaatt agtttcaaca aaggttggca ctgaaaacgg gcaactcctt
     7021 ggtaatactc tcacaggtaa tgacgcagca aaaggagtcg gtgtactcat agaaggttta
     7081 gcaactagta aaaatcctct aatgacattg aaacctaatg attcaaattc tgtttataaa
     7141 gattacgacc cgagaggcaa agacgataca acaggaggag tttacccgga tcaagataca
     7201 ggtataacat accctctcca tttccaggcc acgctacagc aggatggaac tataccaata
     7261 gaagctggtg agtttaaagc caccagtact ttccaggtaa cctaccctta ataagcccac
     7321 cgcaccgcca tcatctgacg gtgcggtaat tgataaatca ttgcccccac caatgtgact
     7381 gataaaatgc caggcaactc gacatcattt atcaggatac gttatgaaac cgaccaccat
     7441 tgcttcactt cagaaatgta agcaggataa aaagcgtttc gcgaccatca ccgcttacga
     7501 ctacagcttc gcgaaactat ttgctgatga aggacttaac gtcatgctgg taggcgactc
     7561 gctgggcatg accgttcagg gtcacgactc caccctgccg gtgaccgttg ccgatatcgc
     7621 ctaccacact gcctccgtac gtcgtggtgc gccaaactgc ctgctgttgg ctgacctgcc
     7681 gtttatggcg tatgccacgc cggaacaagc ctttgaaaac gccgcaacgg ttatgcgtgc
     7741 cggtgccaat atggtcaaaa ttgagggcgg tgagtggctg gtcgaaaccg taaaaatgct
     7801 gaccgaacgt gccgttcctg tatgtggtca cttaggttta accccacagt cagtgaatat
     7861 tttcggtggc tacaaagttc aggggcgcgg cgatgaagcg ggcgatcaac tgctcagcga
     7921 tgcattagcc ttagaagccg ctggggcaca gctgctggtg ctggaatgcg tgccggttga
     7981 actggcaaaa cgtattaccg aagcactggc gatcccggtt attggcattg gcgcaggcaa
     8041 cgtcactgac gggcagatcc tcgtgatgca cgacgccttc ggcattaccg gcggtcacat
     8101 tcctaaattc gctaaaaatt tcctcgccga aacgggcgac atccgcgcgg ctgtgcggca
     8161 gtatatggct gaagtggagt ccggcgttta tccgggcgaa gaacacagtt tccattaagg
     8221 agtcacgttg tgttaattat cgaaaccctg ccgctgctgc gtcagcaaat tcgccgcctg
     8281 cgtatggaag gcaagcgcgt ggcgctggtg cctactatgg gtaatctgca cgatggccat
     8341 atgaagctgg tcgacgaagc caaagcccgc gccgatgtgg tcgtcgtcag tattttcgtt
     8401 aacccgatgc agttcgaccg cccggaagat ctggcacgtt atccacggac cttgcaggag
     8461 gactgcgaga agctaaacaa acgtaaagta gatttagttt tcgccccttc ggtaaaagag
     8521 atctacccga acggtacaga aacccacacc tatgttgatg ttcctggcct ttcgaccatg
     8581 ctggaaggtg ccagccgtcc gggacatttt cgcggcgttt cgactattgt cagcaagctg
     8641 ttcaacctgg tccagccaga tatcgcctgc ttcggtgaaa aagattttca gcaactggcg
     8701 ctgatccgca aaatggttgc cgatatgggc ttcgatattg agattgtcgg tgtgccaatt
     8761 atgcgcgcca aagacggtct ggcgctgagt tcccgtaacg gttatctgac ggcggaacaa
     8821 cgcaaaatcg cgcccggtct gtacaaagtt ttaagttcga ttgccgacaa attgcaggct
     8881 ggagaacggg atctcgatga aattattgct attgccgggc aagaactgaa tgaaaaaggc
     8941 ttccgcgccg atgatattca gattcgtgat gccgacacgt tgctggaagt ctctgaaaac
     9001 agcaaacggg cagtgattct ggtagccgcc tggcttggcg atgcccgcct gatcgacaat
     9061 aaaatagtcg agctggcgta atacttaact ggcgctacgg ctgatggcgc cagttattaa
     9121 tttaccccac gtcatctgtc ataacacagg taacttaaat aaggttaatt accttatcaa
     9181 tctcagcaag agataaattt gtcatctctg caacgtcttc ccgagacatt cctttactta
     9241 gaagacgcag ggcgaattct tgcctttcct cctgtcttcc ctgctgaatc cccttctcaa
     9301 tccctttctc ttcaaaccac tgcgccagcg tcatcataga ctcccctccc gtttccctgt
     9361 ctctcagcac accataaaac aaatccgctt gttcagtatg accgcgttgc agcatatagt
     9421 tttgcatggc aactaactga cttccgctag tgtacccttc gtcaatcagc gtgaccagtt
     9481 gctccagcaa taacattaag tcgcgctggc gaatatgttt ttgcagtagt tcgagaatcg
     9541 caatccgccg atgttgcatg atttcgtcat ccggtgtgat ggtgatatcc accagcggga
     9601 aaggactgtt atagacgcgt cgtgccagct ccggcgagta aaatatatca aaccagcaca
     9661 ttgagagcgg ataaggtgtg gcctcgccct gataaaacag tatcggcacc accagcggca
     9721 gcttatcgtg atccgcctcc agatgccggt gcatggcagc aatagaataa cgcatcatgc
     9781 gaaaggccat tttcttatcc ggcttgcttt ggtgttcaat cacaacatgc agataaccgg
     9841 gattgccctg catttgcacg gaatagagca cgtccgtgct gtgtcctttc aggctctctt
     9901 caatgaaact ccccgactct aaatgaagcg tgttgaggtc acaaagttct cgtaattcca
     9961 ctggcaaatg tatctccaga aagtcgcgag ccgtctccgc atgcattaaa aattgtttaa
    10021 ataccgcgtc atgcggtgtg gtactcggtg catccatctt ccctgtcttc cgtgttgtct
    10081 tgcgtgaagg cagtatccag tcaaggcgcg ccgtcgtcag ccgcctcttt agtgtttacc
    10141 gagcagcgtt cagagaaatt tcaattcgtt acaggcgata catggcacgc ttcggcgcgg
    10201 tacgcaaact gctgacgcaa atgccatcag atgagtagac actcatcaaa aatcgggcaa
    10261 tactgcgtga gaatttcctc aaagcaggca ccgcctgctt cgttaacgac agggtagaaa
    10321 ggtagaagtt atgattcgca cgatgctgca gggcaaactc caccgcgtga aagtgactca
    10381 tgcggacctg cactatgaag gttcttgcgc cattgaccag gattttcttg acgcagccgg
    10441 tattctcgaa aacgaagcca tcgatatctg gaatgtcacc aacggcaagc gtttctccac
    10501 ttatgccatc gcggcagaac gcggttcgag aattatttct gttaacggtg cggcggccca
    10561 ctgcgccagc gtcggcgata ttgtcatcat cgccagcttc gttaccatgc cagatgaaga
    10621 agctcgcacc tggcgaccca atgtcgccta ttttgaaggc gacaatgaaa tgaaacgtac
    10681 cgcgaaagcg attccggtac aggttgcttg attgttatcc ctgcggctgg ttactcacca
    10741 gccgcgacat cgtctccagc gaatccgttc ttaagatata aagtcgtttt agtaacaacg
    10801 gattatcccc cggttttact ttgcctttca tggttgtcac cgccaggtga aatccggcat
    10861 cgtttgctgc cttcacggct ttgtcattaa atccgccaaa cggatacgaa agataccaga
    10921 catgcggatt aaattgcgcc agagcgcggc gtgaacgtgc aaaatcaaac agaatattgt
    10981 gctcactacg gctcagtaat atgggtcggc gatagccatc cacccgatgc aaaaaatggg
    11041 tatgtgactg aaaatcaaaa acatcgcgaa tttcgttaag ctcagaaacg ctcataaatt
    11101 gcagcgattt tgggttccac ttctgcgggt gacgtttgat gcgcgaggta acaacaaacg
    11161 ccgtcgcctt catgccatat tgtttcaata caggatacgc atagcggctc accgacttga
    11221 ggccatcatc aaaggtaatc accaccgctc gcgcagggag attgatctta ttcttcacgt
    11281 aaccttccag ctgcgccatg ctcagtgtcg cgtatcccct gtcgcgcagc caggccatct
    11341 ggttattgaa agcgcgtacc gaggtggtcg tcgaagtatg gcgaaaacgg gtgttttctt
    11401 catcgcgcag aatatggtga taggttagca ccggcaggcc attatcgggt tgggcatcca
    11461 gcgcgctgat ataggcgagt cgatcgccaa tacggatctg ataccaggtt tgatttaacc
    11521 tgtctttcag tttatgcaaa atcgggtagc gcaaattgtc cgccagtacc ccaaatggcg
    11581 cactgcccgc actcggcgcg ttatagaccg gcgtatcttt ccaggtaatt aagttctgat
    11641 tactcagcgg cttgttgagg tcacccaaac cgtcttcaac tttttgtcgc ccctgaactg
    11701 gctcgagatg ccctttatcg ataaaccctt taccaaagcc aaaattaaat gcgtaataac
    11761 ttgcggcagt gggctccacg gcaatgattt gcccggcccg aatattcccc acggtcacca
    11821 tcttgtcgcc aatttgcgcc cagatcgcag cattttcgat ggtttgcata taacgggcag
    11881 gtaacgcggc actgacactt gcggtaaaca gcatcagcag gagaataaca gcttgtttgt
    11941 acatgaaaaa ataaccgagg gtgaaacaac gcttttattg tagcaaaagc gctataagaa
    12001 tcgtgggtta atactcatac aaattatgtt tgaggacgaa aggcggattt ttttgttgtt
    12061 ggtgccaaag actactcacc tccggcccac ccagttcgac aatacgctcg cgaaactctg
    12121 aactggtcat ggtttcacgg caagccatca tctgttcaat taacggtaac gtgacaccag
    12181 aaatcacttc gcaacgggag tgtttgtggc ttaataatga agccacgcgg tacggtggcg
    12241 ctccggctat atcggttaag aagatgacac cctcaccaga gtccgcttca tgcagagcat
    12301 cgcacatcat gcggctgagc atattagagc ttaacccacg ccagaaattc acggcccggc
    12361 actgaagaag cgccccatgt tttttctcca gcgcatccag tatctcttgc gcccggtcat
    12421 cgtgacaggt aattacccaa cctaacatcg ccttcttcct tagccgaaaa tcagtctacc
    12481 ctgaccattg ttgataaagg gtgatagccg tcaaaactcc tctcccccaa atccagagga
    12541 gaggaaatag ccttagctac gcaaaccacg tccacgttgg atcagcgacc aacagatcaa
    12601 ataaaacgcc acaataaaga ccaccagtac gccaaaggta gtgaccagcg gaacatcatt
    12661 gataccgagg aagccgtagc ggaaaccact gatcatataa acgattgggt tcaggtgcga
    12721 cagcccttgc cagaacggcg gcaacaaagt cagtgagtaa aagaccccac ccaaatacgt
    12781 gagtggcgtt aacacaaagg ttggcaccag gctgatgtca tcgaacgttt tggcaaacac
    12841 gccgttcagc aaacccgcaa gggagaatag caccgccgtg agcaccaggg ttaaggcaac
    12901 gaatacccac gaatgcacct gaaacggcac aaaaaacagt gaaattgccg tcaccagaat
    12961 gccaacaaac agaccacgcg ccacgccacc gccgacatat ccggcaataa tgacgtgagt
    13021 cggaaccggc gctaccagca gctcttcaat attacgctgg aacttggcac caaaaaatga
    13081 tgacgcgacg ttagcgtagg cattggtgat caccgacatc atgatcagcc ccggtacgat
    13141 gaactgcata tagctgaagc catgcatatc gccaatacgc gaacctatca ggttaccgaa
    13201 gataataaag taaagggtca tggtgatgac tggcggcacc agcgtctgca cccagatacg
    13261 cataaagcga tggatctctt tcgcccagat gctttttagc gccacccagt aaagatgcat
    13321 catgcgcgat ctccttgttt ttcattaacc agtgaaacaa acagctcttc cagacggtta
    13381 gctttgttac gcatacttaa tacctgaatg ccctgctcac ttaactgcgt aaatacgctg
    13441 ttgatcccct gctcacgcag cacttcaact tccagcgtcg cggtatcgac cagtcgatac
    13501 tgatagccat cgagcttcgg taacggactt ttcggtgcga gatcgagaat aaaggtttcc
    13561 gatttcagct tagccagcag cgccttcatc gaggtatttt ccaccagctc accgtgttga
    13621 ataatgccga tattgcggca cagcatttct gcttcttcca ggtagtgtgt ggtgagaatg
    13681 atggtggtgc ctttgtcgtt taaatccttc aaaaagcccc acattgagcg gcgaagttca
    13741 atatccacgc ctgcggtcgg ttcgtcgaga atcagtagtt taggttcatg cattaacgca
    13801 cgggcaatca ttaaacggcg cttcatcccg ccagataaca tacgcgcacg ttcgttgcgt
    13861 tttccccata gatcgagttg tttaagatat ttttcgctgc ggatgtacgc ctctttgcgc
    13921 tccacaccgt agtaccctgc ctgatttacc acaatttgct gcacggtttc aaacgggttg
    13981 aagttaaatt cctgcggcac cagtcccaac tgacgtttag cgttcacgac atctttctcg
    14041 agatcgtaac caaatacgct gacccgcccg gaggttttat ttaccagaga gctgataata
    14101 ccgatagtgg tcgatttccc ggccccgttc ggcccgagaa gcgcataaaa atcacccgct
    14161 tcgacctgca aatctattcc acgaagcgcc tgaacgccgc ctggataagt ttttttaagc
    14221 tgttgaagtt ccagtgcaat ggtcataaat ttttacttac cttacgttct tacactttat
    14281 ctgtggttta aatcgtcccg gagttgccct atattagcca aacgtaatta tttggttaca
    14341 ggtcgttaac ctccatgaaa gacatagata cactcatcag caacaatgca ctatggtcaa
    14401 aaatgctggt ggaagaggat cccgggtttt ttgagaaact ggcacaagcg caaaaaccgc
    14461 gctttctatg gattggatgt tccgacagtc gcgttcctgc agaacgttta accggtcttg
    14521 agccgggcga actctttgtt caccgtaatg ttgctaacct ggtcattcac accgacctga
    14581 actgcctttc cgtggttcag tatgcagtgg atgtactcga agttgaacac attattatct
    14641 gtggccacta cggttgcggc ggcgtacaag ccgcagttga aaacccggaa ctggggctta
    14701 tcaacaactg gctgctgcat atccgcgata tctggttcaa acatagctca ttgctcggcg
    14761 aaatgccgca agagcgccgt ctggatacct tgtgtgaact gaacgtcatg gaacaggttt
    14821 ataacctggg ccactccacc attatgcaat cagcgtggaa acgcgggcag aaagttacca
    14881 ttcacggctg ggcctacggc attcacgacg gcttgctgcg tgatctggat gtcacagcga
    14941 ccaaccgcga aacccttgag caacgttacc gtcacgggat ttccaacctc aagctgaaac
    15001 acgccaacca caaataaaaa tgccatgccg gatgcaacac atccggcaac ttcacactta
    15061 ctcgtccagc agaatcactt tgcagatata cggcagatga cggtaacgct gtgcgtaatc
    15121 aatgccgtaa cccaccacaa actcatccgg gatcgagaaa ccgataaatt caaccgggac
    15181 gttcacttca cgacgggacg gtttatccag cagcgtacaa atcgccagcg acttcggttc
    15241 gcgcaggctt aagatctcac gcactttcga cagtgtattc cccgagtcga tgatatcttc
    15301 aacaatcagc acgtccttgc cacggatatc ttcatccaga tctttgagga ttttcacatc
    15361 acgggtggtg gacatgccgc taccgtagct ggaggcggtc ataaagtcga cttcatgaga
    15421 tacctgaact tcacggcaca ggtccgccat aaacataaat gagccacgca gcagaccaac
    15481 cagcaccata tcgctgccgc tgtctttgta acgctcagta atctgacgac ccagttcggc
    15541 gatacgcgct ttaatctccg cttcggggat cattacttct acagtatgtt tcatatctct
    15601 aaccatatga tttaaaaaca aatcacttcg cacgggcagg ttttggcacc gcaacaaagc
    15661 gcaagacacg cagtatacca gtaaaactat tcattcgcgg aatctgttaa taaagctcga
    15721 attgtgatga cgatcacaca tgttaaaacc tataattaat tgctactaaa atattaatga
    15781 attgaaatgg tgtctcttta tggcaattaa caatacaggc tcgcgacgat tactcgtcac
    15841 gctaacagcc ctttttgcag cgctttgcgg gctgtatcta ctcattggcg gaggctggct
    15901 ggtcgcgatt ggcggctcct ggtactaccc tatcgctggt cttgtgatgc tcggcgtcgc
    15961 ctggatgctg tggcgcagta aacgtgccgc gctttggctg tacgcagccc tgctgctcgg
    16021 caccatgatt tggggcgtct gggaagttgg tttcgacttc tgggcgctga ctccgcgcag
    16081 cgacattctg gtcttcttcg gcatctggct gatcctgccg tttgtctggc gtcgcctggt
    16141 cattcctgcc agcggcgcag ttgccgcact ggtggtggca ctgctgatta gcggtggtat
    16201 cctgacctgg gccggattta acgatccgca ggaaatcaac ggcaccttaa gcgccaatgc
    16261 cacacctgcg gaagctatct cacccgttgc cgatcaggac tggcctgcct atggtcgcaa
    16321 tcaggaaggt caacgctttt cacctctgaa acaaattaac gccgataacg tccataatct
    16381 gaaagaagcc tgggtgttcc gtactggcga tgtgaagcag ccgaacgatc cgggcgaaat
    16441 caccaatgaa gtgacgccga ttaaagtggg cgacaccctt tacctgtgta ccgctcacca
    16501 gcgcctgttt gcgctcgatg ccgccagcgg caaagagaaa tggcattacg atcctgagct
    16561 gaaaactaac gagtctttcc agcacgtaac ctgccgtggc gtctcttatc atgaagccaa
    16621 agcagaaacg gcttcgccgg aagtgatggc ggattgcccg cgtcgtatca ttcttccggt
    16681 caatgatggt cgactgattg cgattaacgc tgaaaacggc aaactgtgcg aaaccttcgc
    16741 caataaaggc gtgctcaatc tgcaaagcaa tatgccagac accaaaccgg gtctgtatga
    16801 accgacttcg ccaccgatta tcaccgataa aaccatcgtg atggccggtt cagttaccga
    16861 taacttctca acccgcgaaa cgtctggcgt gatccgtggt tttgatgtca acaccgggga
    16921 gctgctgtgg gcttttgatc ccggcgcgaa agatccgaac gcaatcccgt ctgacgaaca
    16981 cacctttacc tttaactcgc caaactcctg ggcaccagcg gcctatgacg cgaagctgga
    17041 tctggtctat ctgccgatgc gcgtgaccac gccggatatc tggggcggta accgcacacc
    17101 ggaacaggaa cgttatgcca gctcgattct ggcgctgaat gccactaccg ggaaactggc
    17161 gtggagctac cagaccgttc accacgacct gtgggacatg gatcttccgg cacagccgac
    17221 gctggcggac atcaccgtta atggtcagaa agtgccagtt atttacgctc cggcgaaaac
    17281 cggcaacatt tttgtgctcg atcgtcgtaa tggcgaactg gtggttccgg caccggaaaa
    17341 accggttccc caaggtgctg ccaaaggcga ttacgttacc cctactcaac cattctcgga
    17401 actgagcttc cgtccgaaga aagatttgag cggtgcggat atgtggggag ccaccatgtt
    17461 tgaccaactg gtgtgccgcg tgatgttcca ccagatgcgc tatgaaggca ttttcacccc
    17521 gccatctgaa cagggtacgc tggtcttccc gggtaacctg gggatgttcg aatggggcgg
    17581 gatttccgtt gatccaaatc gtgaagtggc gattgccaac ccaatggcac tgccgtttgt
    17641 ttcgaaactg atcccgcgtg gtcctggtaa cccgatggag cagccgaaag atgccaaagg
    17701 cacgggtacg gaatccggca ttcagccaca gtacggtgta ccgtatggtg tcacgctcaa
    17761 cccgttcctc tcaccgtttg gtctgccatg taaacagcca gcatggggtt atatctcggc
    17821 gctggatctg aaaaccaatg aagtggtgtg gaagaaacgt attggtacgc cgcaggacag
    17881 tatgccgttc ccgatgcctg taccggtgcc gttcaatatg ggtatgccga tgctgggcgg
    17941 gccaatctcc acggcgggta acgtgctgtt tatcgccgct acggcagata actacctgcg
    18001 cgcttacaac atgagcaacg gtgaaaaact gtggcaaggt cgtttaccag cgggtggtca
    18061 ggcgacgcca atgacctatg aagtgaatgg taagcagtat gtggtgatct ccgcaggcgg
    18121 tcacggttca tttggtacga agatgggcga ctatattgtg gcttatgccc tgccggatga
    18181 tgtgaagtaa gacttgctca gattgctgac aacgtgcgcg ttgttcatgc cggatgcggc
    18241 gttaacgcct tatccagcct acaaaattgt gcaaaatcaa tggattgcac aggtagcgta
    18301 ggcctgataa gcgtagcgca tcaggcagat ttgcgtttgt catcagttta atgcccggag
    18361 agatccgggc atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg
    18421 tatcttcatg ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcatcgt
    18481 gattaaactt caccagcact tcgctgacat taccttctac cttaacggta tctttccagc
    18541 ccgcgcgatg agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt
    18601 gaatatggaa cggatgcagc atcatgtcac ccacgccaga gataacccaa cgttcgtact
    18661 gccctttcgc cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat
    18721 tggcatggtg gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca
    18781 tatggcccat catctggctg tgatccatcc cggccatcgc ctgatcgcca tatttctcca
    18841 tcagcatctg catacccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta
    18901 ccgtcagccc ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac
    18961 cggaggcact aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg
    19021 caatcgccat ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt
    19081 taacctccac cagcacttca aaacgctcgc ccatcagcac cggcagctcg ttcaccttca
    19141 ccggttcagg tagcagacca ccgtcgctgg caatcacata aagcgggcga ttgtcgctgg
    19201 tggcgaaatt gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac
    19261 gcggggcagc gtgttgcggg tagattgcac cgttggtcag caacgtatcg ccaaaccagc
    19321 ccacggcggc ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct
    19381 tatcctgaac gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca
    19441 ggatctcgtc atcttcaatt accaccaacc cagccaaccc catcgccacc tgtcgcccgg
    19501 tcttgccatg ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg
    19561 tcaccgagcg cttgccacct ggcggaataa ttccctgcgg gccgccgtcc acttcacccg
    19621 gtacttccag cccgtgccag tgcaacgtcg tctcttccgt cagttggtta tagatatcaa
    19681 ccgttaccgc tttgccgtgc tgtaatttca ccgccggccc cagcagattg ccgttatagc
    19741 cccaggtagt tgcagttttc ccgccaaagg tggactggcc tgcgccaata gttaactgaa
    19801 tgcgattacg ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg
    19861 caaagactgc gcggctccac agcggcaagg ctgaagccac acccagcgcg acggaatatt
    19921 ttaagaaatc acggcgttgc atagttattt ccttattctt aagcaggcga aacaaaatca
    19981 aacgttgagc atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga
    20041 caataatggt cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt
    20101 agaagcaatg aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtctgcgc
    20161 aaacagttac gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt
    20221 tgtctttctg aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc
    20281 actggtcttt ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat
    20341 gaaagccctc ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa
    20401 aaagtgcaaa gccctggctc gcgattcctt aagcctgctt gcctacgtca aataatccct
    20461 gatacctttt tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc
    20521 gccctttttt tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg
    20581 gcatgaaacg ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca
    20641 tgaaaagacc gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat
    20701 ggcgctggat ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat
    20761 gactcatgtt ccgctactga cccacggtca cgcgaaacat gtgctgatta tcggcggcgg
    20821 cgacggtgcc atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt
    20881 ggaaatcgat gcgggtgtcg tatcgttctg ccgtcagtat ctacccaacc ataacgccgg
    20941 tagctacgac gatccgcgct ttaagctggt gatcgatgat ggcgtcaatt tcgttaatca
    21001 aaccagccag acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga
    21061 aagccttttc acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat
    21121 cttcgtcgca caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg
    21181 caaactcagc cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta
    21241 cggcggtatc atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac
    21301 cgaaattatt caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc
    21361 aatccatacg gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc
    21421 gtcctaagga gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac
    21481 caaaagtctg agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga
    21541 cggttatatt gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc
    21601 agaaacctgt tccattatcg gggccaatat tcttaacatc gcccgtcagg attacgaacc
    21661 acagggtgcc agcgtcacta ttctggtgag tgaagaaccg gtcgacccga aacttatcga
    21721 caaaacagaa caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca
    21781 tatttgcgta catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc
    21841 cgatattgaa gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat
    21901 ccaccagctt gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga
    21961 cattaacggt atgaaacact ttatcgacca tgagattaat tcgattcaga actttatgtc
    22021 tgacgatatg aaggcgctgt atgacatggt ggatgtaaat gtctatcagg aaaatatctt
    22081 ccataccaag atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc
    22141 ggaagattta accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg
    22201 cgagatttat tacgggcgca atatgccagc tgtttaacgt ctcgggcgga gctcccaggc
    22261 tccgccagat ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga
    22321 aaatcctcaa cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccttcttcc
    22381 atttcatcgc cagcgaattc cagttgattg gcgcgaacca tcacctcttc accgtccatc
    22441 cacagggtgt attcatgccc tgcccgctgc caggaacgtt cgctaccttt cagtgcgtgc
    22501 gccgcttgtt ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag
    22561 tgcccgacca cttcatgccc catggacata cgcaccttta caactccggt aatatcgcgc
    22621 agaaattcgt aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcattgc
    22681 gccattgatg taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag
    22741 aaataaaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg
    22801 tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg
    22861 agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc
    22921 cacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc
    22981 cagttccgca gaagccagga agacattcgc gccagtaccc agacggttcg ggaagttacg
    23041 ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag
    23101 ggaacagcca gggatctcga tacgcgcacc actcttaccg aagacgctgt agtagccttc
    23161 ttcagtcaac tgcgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcag
    23221 ttggcctttg tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttggtcat
    23281 gcaggaaccg ataaatactt cgtcgatctt ctcaccctgt accgcagaca gcggacgcgc
    23341 gtcatccggg tcgttcggtg cacagaggat tggctcttta atatccgcca gatcgatgtc
    23401 gatcactgcc gcgtattcag catctgcatc ggcttccagc agctcaggat tcgccagcca
    23461 tttttccatg ccctgaatac gacgttccag ggtgcgacga tcgccgtaac cttccgcgat
    23521 catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag
    23581 cttgatggta caaccagcgg cagaacgctc ggcggacgca tcggtcagtt caaatgcctg
    23641 ctcaactttc agatccggca gaccttcaat ttccaggatg cggccagaga agatgttttt
    23701 cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg gaatggcatg
    23761 taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga
    23821 ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc
    23881 cgccgggaaa gagataccga tcgggaaacg ggtatgggaa tcaccaccgg taccgacggt
    23941 atccggcagc agcatacggt tcagccagga gtgaatgacg ccgtcacccg gacgcagcga
    24001 cacaccgcca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg
    24061 cttcggatac gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag
    24121 gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct gggaacctac
    24181 agaagtcatt ttcggttcac agtacgcgcc cggacgaatg cctttcacgc cacaggcacg
    24241 gcctaccatt ttttgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg
    24301 acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg
    24361 cccgataatc agcggaatac ggccaccagc acgcacttca tcaatcagca cgtcggtttt
    24421 cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac ctttgtacgg
    24481 gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc
    24541 acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc
    24601 accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt
    24661 agtggcggat ttacgcgaag aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa
    24721 acctttctgt tgcagagctt cgatttgctt gatcggacca acaacaccag gctggtctgg
    24781 ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg
    24841 tgaccacgca tccggtgccg gagaaaggtc atcggtgtta gtttcgccag tgactttgaa
    24901 gacggtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc
    24961 ccaggactgc ataacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc
    25021 atagaagtta tcgaacatca gcagcgtgtg agaaagtgct ttggcagcaa taggtgccag
    25081 tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc
    25141 cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttcgcgat
    25201 agcagccagg aagcctgctt tgacataggc ggcttcatcg acgcctgggg gaacacggtt
    25261 ggttaacaga tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac
    25321 aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc
    25381 agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt
    25441 gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt
    25501 accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac aaaaaagcaa
    25561 cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact
    25621 tcgttaattc gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt
    25681 aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt cgccgaccat
    25741 tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg
    25801 gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat aaaaagccgc
    25861 acagtggaag cgcctgtgga accgcaatcg acacaacaga ctgcgcctga catcaccgca
    25921 gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt caaatccgca
    25981 ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc cgattctctg
    26041 ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg
    26101 tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg
    26161 tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa
    26221 aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg
    26281 gactcggtac agcgattgcg gcaactggcg caacaaaccg gctcgctgaa gtcatcaacc
    26341 gaacagaaag ttattaccac aacgaagaaa gctgtaccgg taacacagac agtcacggct
    26401 cccgtcatac catccaatac cgtttcaact gccagccccg tcattacaga gcctgcaaca
    26461 accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca
    26521 accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca
    26581 gccggagaac cgtttattga cagctttgtg cgcggattcg gctatagcat gggtgttgct
    26641 accacgtacg cactattcag cagcatcgac tgggacgacg acgatcatga ccatcatcat
    26701 catgacgatg atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa
    26761 cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat
    26821 cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat
    26881 catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagcgcc
    26941 acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag
    27001 caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag
    27061 cggtttaatg aagctgaaaa ctatgggagc tatgacgact tccgcgactt cagccgtcgc
    27121 caaccactga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc ggcctcgcct
    27181 gagcagcgcc aggcagtccg cgagaaaatg cagactaacc cacagatcca gcagcgaaga
    27241 gacgcagcgc gtgagcgtat tcagtccgcc tcgcctgagc agcgccaggc agtccgcgag
    27301 aaaatgcaga ctaacccaca gatccagcag cgaagagacg cagcgcgtga gcgtattcag
    27361 tcagcctcgc ctgagcagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac
    27421 caacagcaac gtgataacgc ccgccagcgt gttcaatcag catcacctga acaacgtcag
    27481 gtttttcggg aaaaagttca ggagagccgc ccacaacgtc taaacgacag taaccatact
    27541 gtcaggctga ataacgagca acggtcagca gtacgcgaac gtctctctga gcgcggagca
    27601 aggcgacagg aaaggtaaat tgcagacgta aaaaaagcgg cgtggttagc cgctttttta
    27661 attgccggat gttccggcaa acgaacaatt acttcttctt cgctttcggg ttcggcaggt
    27721 cggtaatgct accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt
    27781 gcgcgtggat ggtcagtgcg atgtcttcag catcacaacc catttcgatt gccaggccga
    27841 tttcacccag cagctcgccg ccgttggtac cgacaatcgc accaccgatc acacggtgag
    27901 attctttgtc gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac
    27961 cagaagcagc ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt
    28021 tctcagtcag acctacccat gcaacttctg gttcggtata ggcgatggac gggataactt
    28081 tcggatcgaa gtagtgtttc ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa
    28141 cacctttgtg tgccagcatc ggctgaccga cgatatcgcc gatagcaaag atgtgcggta
    28201 cgttggtacg cagctgtttg tcaacgcgga tgaaaccacg gtcgtccact tccacgccag
    28261 ctttgcctgc gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt
    28321 cgtaacgctg cggttcagcg ggtgcttttt tgccttccat cgtcacataa ataccgtctt
    28381 ctttcgcttc aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac
    28441 gcttggtgaa gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt
    28501 caaccacgtc aatctgtgaa cccagcgcgt ggtatacggt gcccatttcc agaccgatga
    28561 taccgccacc cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg
    28621 agtcccagat acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg
    28681 ctgcaatgat cgcgttgtcg aagttgatca cggttttacc gttctcacct tcaacttcca
    28741 gggtgttagc cccggtaaat ttacccagac cgttgaccac tttgactttg cggcctttcg
    28801 ccataccagc cagaccaccg gtcagctgat tgatcacttt ctctttccag gtacgaatct
    28861 tgtcaatatc ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt
    28921 cttcgataac ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc
    28981 aaacaccgcc aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc
    29041 aacggaaggc agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga
    29101 tttcagtact catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc
    29161 aggccgcagc ggcgttagct tcacgccctc accccagtca cttacttatg taagctcctg
    29221 gggattctgg cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata
    29281 ccctaaccac caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt
    29341 aaacaacaaa cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt
    29401 accagaaaaa agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt
    29461 cagacagcgt gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc
    29521 ggtggtcgaa ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc
    29581 acaccggctc catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa
    29641 tcggcgcgaa gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct
    29701 gcatttcgcc cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc
    29761 gagacagctc gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt
    29821 tcggggtatc caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt
    29881 cttccgacaa cgaactgttg aagcgaggca tctgctcaag agctgcagca acggctttca
    29941 tgatgaagac aaccggggtg atcttcacat ccagcttacg tttcgccgct tcttcgttct
    30001 gctgtttacg gaacgcttcc agctcggtga tatcggtttt gtcgaagtga gtaacatgcg
    30061 ggatcatcac ccagttacgg ctcaggttcg caccagaaat tttctggata cggcccagtt
    30121 ccacttcttc gatttcacca aacttgctga agtccacctt cggccacggc agcatgcctg
    30181 ggataccgcc gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt
    30241 aagcctgaac gtcttcgcgc aggatacgac ctttacggcc agtgcccttc actttcgcca
    30301 ggttaacgcc aaactcgcgt gccagacggc ggatcagcgg agtcgcgtga acgtaagcgt
    30361 cgttttctgc aaattcagat ttgccttccg ctttcgcagc cggtgctgct gccggagctt
    30421 cagcttttgc tgccggagcc ggcgctgccg cttcctgttt cgcaggagct gccgcaggcg
    30481 ctgcgccttc aacttcgaag atcataatca gcgagccagt tttcacttta tcgccaacgt
    30541 tgactttcag ttccttcacg acgcctgcaa acggagccgg aacttccata gaagctttgt
    30601 cgccttctac ggtaatcagt gactgttcag cggcaacttt gtcgcccact ttcaccatca
    30661 cttcggtcac ttcaacttcg tcactgccga tatccggaac gttaacttct ttcacgccag
    30721 cagctggtgc aggggccgct gccggagccg cttcctgttt agccgccgga gctgccgcgc
    30781 ctgcttcacc cgcgacttcg aagaccataa tcagcgagcc ggtagacact ttgtcaccca
    30841 cgttcacttt gatctctttc acggtgccag caaacggagc cggaacttcc atagacgctt
    30901 tatcgccttc tacggtgatc agcgactgtt cagcttcaac tttatcgcca actttcacca
    30961 ggatttcggt cacttcaact tcgtcgctgc cgatatccgg aacgttaacg tcttttgccg
    31021 ctgcagccgc tggtgctgct gccggagctg cttctttctt ctcttctgcc tgagcaggtg
    31081 cagcgtctgc tgcaccgtcg gcggaatcga aaatcataat cagtgcgccg gtctgggttt
    31141 tatcgccaac agagactttg atctctttaa cgatacccgc ctgcggagac ggaacttcca
    31201 tagaggcttt gtcgccttct acggtgatca gcgactgttc ggcttcaact ttgtcgccca
    31261 ctttgaccag gatctcggtg atttcaactt catcagcccc gatgtccggt actttgattt
    31321 cgatagccat tattctttta cctcttacgc cagacgcggg ttaactttat ctgcatcgat
    31381 gttgaatttg gcgattgcgt cagcaaccac tttcttatcg atttcgccac gtttagccag
    31441 ttcgcccagc gccgctacaa ccacgtagga agcatcaact tcgaagtggt gacgcaggtt
    31501 ctcacggctg tcggaacgac cgaagccatc agtacccagt acgcggtagt cgtcagccgg
    31561 tacgtaagta cggacctgct cagcgaacag tttcatatag tcggtagatg ccactgccgg
    31621 agcgtcgttc atcacctgag cgatatacgg aacgcgcgga gtttccagcg ggtgcagcat
    31681 gttccagcgt tcacaatcct gaccatctcg cgccagttcg gtgaaggagg tcacgctata
    31741 aacgtcagaa cctacgccgt aatctttcgc caggatctct gccgcttcac ggacgtgacg
    31801 caggatagaa ccggagccca gcagctgaac tttacctttg ctaccttcaa tggtttcgag
    31861 tttgtagata cctttacgga taccttcctc agcaccttcc ggcattgccg gcatgtggta
    31921 gttttcgttc agcgtggtga tgtagtagta aacgttctct tgtttttcac cgtacatacg
    31981 ctccagaccg tcatgcataa tgacagcaac ttcgtaagcg taagccgggt cgtaagagat
    32041 acagttcgga atagtcagcg actgaatgtg gctgtgacca tcttcgtgct gcagaccttc
    32101 gccgttcagg gtggtacgac cggaagtacc gccgatcagg aagccacgcg cttgctggtc
    32161 gccagccgcc cagcacagat cgccaatacg ctggaagccg aacatcgagt aatagatgta
    32221 gaacgggatc atcggcagat tgttggtgct gtaagaggtc gccgctgcca gccaggaaca
    32281 acctgcgccc agctcgttga tcccttcctg cagaatctga cctttctcgt cttctttata
    32341 gtaagcaacc tgctcgcggt cctgcggggt gtactgctga ccgttcgggc tgtaaatacc
    32401 aatctgacgg aacagacctt ccataccgaa agtacgcgct tcgtcggcga tgatcggtac
    32461 cagacgatct ttgatcgact tgttcttcag cattacgttc agggcacgaa cgaaagcgat
    32521 agtggtagag atctctttgc tctgctcttc cagcagcgcg ccgaagtctt gcaggctcgg
    32581 cagctcaagc ttctcggtga agttcggctg acggcttggc agataaccgt gcagtttctg
    32641 acgctgtgcg tgcagatagg tatgctcttc agaaccttcc gggaaggtga tgtacggcag
    32701 tttttcgata tctgcatcag acaccggcac attgaaacgg tcgcggatgt gacgcacgcc
    32761 gtccatgttc attttcttaa cctggtgcgc gatgttttta ccttcagccg cgtcgcccat
    32821 gccgtaacct ttaatggtat gagcaaggat tactgtcgct ttgcctttgg tttcctgcgc
    32881 tttcttgaat gcagcgtaga ttttcttcgg atcgtgacca ccacggttca gtgcccagat
    32941 ctgctcgtca gtccagtctg caaccagtgc tgcggtttca ggatatttac cgaagaagtg
    33001 ttcacgaacg tacgcaccat ctttcgattt gaaggtctgg tagtcgccgt caacggtttc
    33061 gttcatcagc tggatcagtt taccgctggt atctttacgc agcagttcat cccaacggct
    33121 accccacatc actttgatca cgttccagcc agcaccttcg aagatgcctt ccagttcgtt
    33181 gatgatcttg ccgttaccgg tgaccgggcc gtcaagacgc tgcaggttac agttgataac
    33241 gaagaccagg ttatccagtt tttcacgggt agcgatggtg atcgcacctt tggattccgg
    33301 ttcgtccatt tcaccgtcgc cgaggaacgc gtaaacggtc tgtttagagg tatctttcag
    33361 gccacggtgt tccagatatt tcaggaattt agcctggtaa atagcaccaa tcggccccag
    33421 acccatagat acggtcggga actgccagaa ttccggcatc agtttcgggt gcggatagga
    33481 agagaggcca ttgccgtgaa cttcctgacg gaagttatcc agctgctcct gagtcagacg
    33541 accttccagg aaagcacgag cgtacacgcc cggggagatg tggccctgga agtaaaccag
    33601 gtcgccgcca tcctgctcgt tgcgtgcacg gaagaagtgg ttaaagcaca catcataaat
    33661 ggttgcggaa gactggaagg acgccatatg gccgcccagt tcgaggtctt ttttcgacgc
    33721 acgcagcacc gtcatgatgg cgttccagcg gatagctgaa cgaatacggc gttccagttc
    33781 cagattaccc ggatactccg gttgttcttc aacggggatg gtgttgatgt agttgctgat
    33841 acctgtgcct gcggctacgt ttacaccgcc tttgcgggct tcagcaagca gttggtcgat
    33901 cagatactga gcacgctcaa caccttcttc acggatgacc gattcgatcg cctggagcca
    33961 gtcgcgagtt tcgatcggat ccacgtcatt tgggaaacgt tctgacatgg gttattcctt
    34021 atctatctaa taacgttgag ttttctggaa cctgtcccat tgaactctcg ccggaaagct
    34081 caataagaca ggttctacgt ttagttgccg cgcttttata tgcgcttgat ttacaacatc
    34141 ttctggataa tttttaccag aaaaatcact aattctttcg ttgctccaga cgacgcagag
    34201 aacgctcacg gcggctctct tcacgacttc tgtcgagcaa aatttcttcg ataaaggcca
    34261 gatggcgatg cgatgcttcg cgcgcttctt ccggcttacc ggccataatc gcttcaaata
    34321 tgcgggtgcg gtgactactc accagcggca gcatctcgcg acgcgaatag agcaattcga
    34381 agttctggcg gacattctgg gccaacatcg gctccataca ccttagcaga tgaagcagaa
    34441 ccacattgtg ggccgcttca gtgacagcaa tctgatactg gagtacggcg tttgattccg
    34501 cgtccagatc gccagactgc tgcgccagct ctatggcgtg gtggagttca cggatgcgtt
    34561 ccttgtcttc atcggtacta cgcagcgcgg cgtaataagc ggcgatacct tccagggcgt
    34621 gtcgtgtttc gagcaagtca tactgtgact caggatggtc ggagagcagc tccaccagcg
    34681 gatcgctgaa gctttgccat aggctgctct ggacaaaagt gccgccaccc tggcgacgaa
    34741 gcaacaagcc cttcgcttcg agacgttgaa tcgcctcacg caaggaggga cgggagacgt
    34801 caaactgttt tgccagttcg cgttccggtg ggagtttttc gcccgggcgg agagtgcctt
    34861 cgaggatcaa aaactccagt tgctgctcaa tcacatcgga gagttttggt tggcggattt
    34921 tgctgtaggc catgagttcc tgtcttaagc cacttgccga agtcaattgg tcttaccaat
    34981 ttcatgtctg tgacgctaaa gtaacaaagt attcacctta tgtccataca ggttttgatt
    35041 gaaatcatga aactgtgcac attttaacaa cttgacatat ataacgt
//
LOCUS       DAZGXZ010000042        34952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000042 DAZGXZ010000000
VERSION     DAZGXZ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.042"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            201..914
                     /gene="ydiV"
                     /locus_tag="ACHKJF_002127"
     CDS             201..914
                     /gene="ydiV"
                     /locus_tag="ACHKJF_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP0949485.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            977..2413
                     /gene="selO"
                     /locus_tag="ACHKJF_002128"
     CDS             977..2413
                     /gene="selO"
                     /locus_tag="ACHKJF_002128"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP0949486.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(2417..2608)
                     /gene="hemP"
                     /locus_tag="ACHKJF_002129"
     CDS             complement(2417..2608)
                     /gene="hemP"
                     /locus_tag="ACHKJF_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP0949487.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2740..3786)
                     /gene="aroH"
                     /locus_tag="ACHKJF_002130"
     CDS             complement(2740..3786)
                     /gene="aroH"
                     /locus_tag="ACHKJF_002130"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP0949488.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(3943..4776)
                     /gene="ppsR"
                     /locus_tag="ACHKJF_002131"
     CDS             complement(3943..4776)
                     /gene="ppsR"
                     /locus_tag="ACHKJF_002131"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP0949489.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            5109..7487
                     /gene="ppsA"
                     /locus_tag="ACHKJF_002132"
     CDS             5109..7487
                     /gene="ppsA"
                     /locus_tag="ACHKJF_002132"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP0949490.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(7544..9190)
                     /gene="fadK"
                     /locus_tag="ACHKJF_002133"
     CDS             complement(7544..9190)
                     /gene="fadK"
                     /locus_tag="ACHKJF_002133"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP0949491.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(9247..9540)
                     /gene="ydiT"
                     /locus_tag="ACHKJF_002134"
     CDS             complement(9247..9540)
                     /gene="ydiT"
                     /locus_tag="ACHKJF_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0949492.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(9537..10826)
                     /gene="ydiS"
                     /locus_tag="ACHKJF_002135"
     CDS             complement(9537..10826)
                     /gene="ydiS"
                     /locus_tag="ACHKJF_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0949493.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALIENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(10882..11820)
                     /gene="ydiR"
                     /locus_tag="ACHKJF_002136"
     CDS             complement(10882..11820)
                     /gene="ydiR"
                     /locus_tag="ACHKJF_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP0949494.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(11840..12604)
                     /gene="ydiQ"
                     /locus_tag="ACHKJF_002137"
     CDS             complement(11840..12604)
                     /gene="ydiQ"
                     /locus_tag="ACHKJF_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP0949495.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            12920..13831
                     /gene="ydiP"
                     /locus_tag="ACHKJF_002138"
                     /pseudo
     CDS             12920..13831
                     /gene="ydiP"
                     /locus_tag="ACHKJF_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(13874..15025)
                     /gene="ydiO"
                     /locus_tag="ACHKJF_002139"
     CDS             complement(13874..15025)
                     /gene="ydiO"
                     /locus_tag="ACHKJF_002139"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP0949496.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESPLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECTFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(15039..16634)
                     /gene="ydiF"
                     /locus_tag="ACHKJF_002140"
     CDS             complement(15039..16634)
                     /gene="ydiF"
                     /locus_tag="ACHKJF_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP0949497.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMNERLFIDAAMGFVLPEAAH"
     gene            complement(16778..17536)
                     /gene="aroD"
                     /locus_tag="ACHKJF_002141"
     CDS             complement(16778..17536)
                     /gene="aroD"
                     /locus_tag="ACHKJF_002141"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP0949498.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(17567..18433)
                     /gene="ydiB"
                     /locus_tag="ACHKJF_002142"
     CDS             complement(17567..18433)
                     /gene="ydiB"
                     /locus_tag="ACHKJF_002142"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP0949499.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(18445..19710)
                     /gene="ydiN"
                     /locus_tag="ACHKJF_002143"
     CDS             complement(18445..19710)
                     /gene="ydiN"
                     /locus_tag="ACHKJF_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949500.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     IRPIWANVFNSALATITAAIIYRYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(19937..21151)
                     /gene="ydiM"
                     /locus_tag="ACHKJF_002144"
     CDS             complement(19937..21151)
                     /gene="ydiM"
                     /locus_tag="ACHKJF_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949501.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(21251..21607)
                     /gene="ydiL"
                     /locus_tag="ACHKJF_002145"
     CDS             complement(21251..21607)
                     /gene="ydiL"
                     /locus_tag="ACHKJF_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP0949502.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(21743..21850)
                     /gene="rprA"
                     /locus_tag="ACHKJF_002146"
     ncRNA           complement(21743..21850)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKJF_002146"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(22036..23148)
                     /gene="ydiK"
                     /locus_tag="ACHKJF_002147"
     CDS             complement(22036..23148)
                     /gene="ydiK"
                     /locus_tag="ACHKJF_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP0949503.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            23537..26593
                     /gene="ydiJ"
                     /locus_tag="ACHKJF_002148"
     CDS             23537..26593
                     /gene="ydiJ"
                     /locus_tag="ACHKJF_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP0949504.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            26590..27000
                     /gene="menI"
                     /locus_tag="ACHKJF_002149"
     CDS             26590..27000
                     /gene="menI"
                     /locus_tag="ACHKJF_002149"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP0949505.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            27100..27288
                     /gene="ydiH"
                     /locus_tag="ACHKJF_002150"
     CDS             27100..27288
                     /gene="ydiH"
                     /locus_tag="ACHKJF_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP0949506.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            27836..28204
                     /gene="sufA"
                     /locus_tag="ACHKJF_002151"
     CDS             27836..28204
                     /gene="sufA"
                     /locus_tag="ACHKJF_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP0949507.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            28213..29700
                     /gene="sufB"
                     /locus_tag="ACHKJF_002152"
     CDS             28213..29700
                     /gene="sufB"
                     /locus_tag="ACHKJF_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP0949508.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            29710..30456
                     /gene="sufC"
                     /locus_tag="ACHKJF_002153"
     CDS             29710..30456
                     /gene="sufC"
                     /locus_tag="ACHKJF_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP0949509.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            30431..31702
                     /gene="sufD"
                     /locus_tag="ACHKJF_002154"
     CDS             30431..31702
                     /gene="sufD"
                     /locus_tag="ACHKJF_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP0949510.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            31699..32919
                     /gene="sufS"
                     /locus_tag="ACHKJF_002155"
     CDS             31699..32919
                     /gene="sufS"
                     /locus_tag="ACHKJF_002155"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP0949511.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            32932..33348
                     /gene="sufE"
                     /locus_tag="ACHKJF_002156"
     CDS             32932..33348
                     /gene="sufE"
                     /locus_tag="ACHKJF_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP0949512.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            33497..34501
                     /gene="ldtE"
                     /locus_tag="ACHKJF_002157"
     CDS             33497..34501
                     /gene="ldtE"
                     /locus_tag="ACHKJF_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP0949513.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(34565..34801)
                     /gene="lpp"
                     /locus_tag="ACHKJF_002158"
     CDS             complement(34565..34801)
                     /gene="lpp"
                     /locus_tag="ACHKJF_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP0949514.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         8868 a         8572 c         8887 g         8625 t
ORIGIN      
        1 ctgaatacag tagtattttg cagacaaaaa ttccgtaaat ggattattcg tttataatca
       61 ggataatcta aaaaaaggac ccctgaaaaa gtggttaaca tggaacaaat gaaagttact
      121 ccttctgtaa ttgttatgtc ttatggcctc atccctttta gccggatact gaaaaacatc
      181 cttcgagagg gacggttacc atgaagattt ttttggagaa tctttatcat tctgattgct
      241 atttcctgcc gataagagat aaccagcaac tccttgtcgg cgttgagtta attacccatt
      301 tctccagtga agatggcacg gtgcgtatcc cgacgagtcg ggttatagcg caacttaccg
      361 aggagcaaca ttggcaatta ttttcagagc agttggaatt actaaaatca tgccaacatt
      421 tttttattca acataaactc tttgcctggc ttaatttaac gccacaagtg gctacattat
      481 tattagagag ggataattac gcaggcgaac tattaaaata tccgtttatt gaactgttga
      541 ttaatgagaa ttacccgcat ctcaatgaag gaaaagataa tcgcggtctt ttatccttat
      601 cgcaagtgta tccccttgtg ttaggtaatt tgggagcagg caatagcaca atgaaagcgg
      661 tttttgatgg tcttttcacc cgcgtcatgc tggataaaag ttttattcag caacaaatca
      721 cgcaccgctc cttcgaaccg tttattcgtg cgatccagtc gcaaatttcc ccttgttgca
      781 attgcatcat tgcgggaggc attgatacgg cagaaatatt ggcacagatt acccccttcg
      841 atttccatgc tctccaggga tgcctgtggc ctgccgttcc cataaatcag ataacgacgt
      901 tggttcagcg ataacccttc tgtttgctgg tgtttaagac gagagtaacc gtctacacta
      961 tcaaacagga ggatctatga ccctgtcttt tattacccgc tggcgcgatg aattgccaga
     1021 aacctataca gcactttccc ctacgccttt aaataatgcc cggctgattt ggcataatac
     1081 cgaactcgct aacacgctga gtattccatc gtcgctgttt aaaaatgctg caggtgtctg
     1141 gggcggcgaa accctactgc ctggtatgtc accactggcg caggtttaca gtggtcatca
     1201 gttcggcgtc tgggcgggcc aactgggtga tgggcgcggc attttacttg gcgaacaact
     1261 gcttgctgat ggcactacaa tggactggca tctgaaaggt gctggcctga cgccttattc
     1321 gcgaatgggt gatggacggg cggttttacg ttcgacgata cgagaaagtc tcgccagtga
     1381 ggcgatgcat tatctgggca ttccgacgac ccgcgcgtta agtatcgtca ccagcgattc
     1441 gccagtgtat cgggaaacgg tggagccagg cgcgatgctg atacgtgtgg caccaagtca
     1501 tctgcgcttt ggtcatttcg aacattttta ctatcgccgc gagccggaaa aggttcgtca
     1561 gttagctgac tttgccattc gtcattactg gtcacatctt gaagatgatg aggacaaata
     1621 ccgtctctgg tttaacgatg ttgtcgcacg taccgcatcg ttaattgccc aatggcagac
     1681 ggtcggcttt gctcatgggg tgatgaatac cgacaacatg tcgctgctgg ggctgacgct
     1741 tgattacggg ccgtttggtt ttcttgatga ttacgaaccc ggttttattt gtaatcactc
     1801 ggatcatcaa gggcgttaca gctttgataa tcaacctgcc gtcgcgttgt ggaatttaca
     1861 gcgtctggcg cagacattgt caccatttgt tgccgtagat gccctgaatg aggccctgga
     1921 cagctatcag caggttttgt tgacgcatta tggacaacgg atgcggcaga aactgggctt
     1981 catgacggag caaaaagagg ataacgcgct actgaatgaa ttattcagtc tgatggcgcg
     2041 agagcgcagc gattataccc gcacattccg catgctgagt ctgaccgagc agcacagcgc
     2101 ggcgtcaccg ctacgtgatg agtttattga tcgtgcggca tttgatgact ggtttgcccg
     2161 ttatcggagg cgtttgcaac aagacgaggt tagcgatatt gagcgtcagc aactgatgca
     2221 aagcgttaac ccagctctgg tgttgcgcaa ctggttggcg caacgggcga ttgaagcggc
     2281 agaaaagggt gatatgacgg aattgcaccg cctgcatgag gcgttgcgaa atcctttcag
     2341 cgacagagct gatgactatg tcagtcgtcc acctgactgg ggtaaacggc tggaagtcag
     2401 ctgctcgagt taaaggctac ttagttaaca gcagcttgcc agcctgagtt ttacggagca
     2461 gatattcttg cccgtcatga tcgataatca gtttgccatc cggacctaac agtgtctggc
     2521 tggaaattcg ccgaataggc tgcggggaag cggttttgtg attggcatcc gtttcaggcg
     2581 tgagttttct gctatccgta taacgcataa aaattaataa taattgatat aacaatgatt
     2641 cttattatca atattatggc tgaccagagc aagcagtaat gtgctgagaa gacaacaagt
     2701 tgcgcagaaa tgcccatccc atcaggaatg ggcacgcatt cagaagcggg tatctaccgc
     2761 agaggcaagt ttttcgacca ggcgttcggt atcttcccag cccagacacg ggtcggtaat
     2821 ggattgaccg taagtgagcg gctgaccgcc gacgattttt tgcgttcctt cgcgcaggaa
     2881 actttccgcc ataattccag caatcgccgt agagccattg cggatttgct gacaaatatc
     2941 ctcacaaact tctaactggc gacggtgctg cttctggcag ttaccgtggc tgaaatccac
     3001 caccagatgt tcaggtaaat caaactcgtg cagcgtatcg caggctgcgg cgatatcatc
     3061 ggcatgataa ttcggttttt tgccgccacg cataataatg tggccatacg ggttgccgct
     3121 ggtctgatag atggtcatct gaccattttt gtctggcgag agaaacatat ggctggcgcg
     3181 ggctgcgcgg atagcatcca cggcaatccg cgtgttgcca tcggtaccat ttttaaaacc
     3241 taccggacag gagagtgccg aagccatttc acggtggatc tgactttcgg tagtacgtgc
     3301 gccaatcgcg ccccaactga ttaaatcagc aataaactga ccggtcacca tatcgaggaa
     3361 ctcagtcgcg gtagggacac ccagctcatt tacctgtaaa agtaatttgc gcgccagctc
     3421 cagaccgtga tttacccgat agctgccgtt taaatccgga tcggagatta gccctttcca
     3481 gccgacaaca gttcgtggtt tttcaaaata ggtgcgcatt acgatttcca gccgtgactg
     3541 gtactggttg cgcagcgact gcagacgggt ggcgtactcc attgcagcgg tgagatcgtg
     3601 gatcgagcag gggccaataa taaccaacag tcgcttatct tcaccattca gtattttttc
     3661 aattctgcgg cgggagtcgg tgacatgggt ggcgacgcca ggcgttacgg gataccgtag
     3721 cgcgagttcg gcgggcgtta ccaggctctc aatacgcgca gtacggagtt cgtcagttct
     3781 gttcattaca agtctcagaa atttgttgct tctacggcag gactaccggg aagtgatgca
     3841 catcacgata aaccaatcgc agctgatttc aagtgcgagg tgtgtcacac ctcgccgggt
     3901 ggttagcatg ataacaaaaa aataagctaa tgcactagtt ctctagtaca ttcggcggct
     3961 aagccccatg atatcgagga tcttggtggc aatctcttct accgaataat tggtactgtt
     4021 aatccacggg atctgatttt tacggtacaa cgcttctact tccgcgactt ccatcctgca
     4081 ctgacgaagc gaggcatagc ggctgttctc ccgacgttcc tcgcgaatcg ccgccagacg
     4141 ttccgggtcg atagtcaggc cgaacaattt atgctgaagc ggtttgagcg acgcgggtag
     4201 caccagatta tccatatcgt cggcaataaa ggggtagttt gccgcgcgga taccaaattg
     4261 cattgccaga tacagactgg tgggggtttt accacagcga gaaacgccga gcaggatcac
     4321 ctgagcctgg tccagattgc gcaacgaaat accgtcatcg tgggcaaggg tgtaatcaat
     4381 cgccgcaatg cgcgcatcat atttattgag attattaggg ttaaggccat gggtacgatg
     4441 agcaatcggc gttggatcca gtttcatctc ttgttgtagc ggggcaacca gcgcctgaac
     4501 gatatcctgg caaaagcctt cactttgcaa gatgatggcg cgaatctccg gcaacacgat
     4561 ggagtagaag accagcgggc gcacacctgt ctggtgataa attgcgtcaa tctgatcctt
     4621 cactgcccgt gcacggctct cattttcgac aaacggcagc gtaatgctgc tgatagtgac
     4681 gggaaattgt gacattactg cgtgtcctaa tacctccgca gttattgccg taccatcaga
     4741 aatataaaaa acgtggcgat caacagcatt atccattttg ttcttcccgt gatgcagaca
     4801 taattctatg aaagcataaa ttaaaaacgc acagaagcgt agaacgttat gtctggttta
     4861 taaaatgaac cttcaatttt attttttatg aaaacagcat ttcattttta tggtttcgtt
     4921 tataccgatg gtttatgtgg aaattgtcga agagagcaga tttgcgcaac gctgggatca
     4981 gtcttaaaaa gtaaaaaaat atatttgctt gaacgattca ccgttttttt catccggtta
     5041 aatatgcaaa gataaatgcg cagaaatgtg tttctcaaac cgttcattta tcacaaaagg
     5101 attgttcgat gtccaacaat ggctcgtcac cgctggtgct ttggtataac caactcggca
     5161 tgaatgatgt agacagggtt gggggcaaaa atgcctccct gggtgaaatg attactaacc
     5221 tttccggaat gggtgtttcc gttccgaatg gtttcgccac aaccgccgac gcgtttaacc
     5281 agtttctgga ccaaagcggc gtaaaccagc gcatttatga actgctggat aaaacggata
     5341 ttgacgatgt tactcagctt gcgaaagcgg gcgcgcaaat ccgccagtgg attatcgaca
     5401 ctcccttcca gcctgagctg gaaaacgcca tccgcgaagc ctatgcacag ctttccgccg
     5461 atgacgaaaa cgcctctttt gcggtgcgct cctccgccac cgcagaagat atgccggacg
     5521 cttcctttgc cggtcagcag gaaaccttcc tcaacgttca ggggtttgac gccgttctcg
     5581 tggcagtgaa acatgtattt gcttctctgt ttaacgatcg cgccatctct tatcgtgtgc
     5641 accagggtta cgatcaccgt ggcgtggcgc tctccgccgg tgttcaacgg atggtgcgct
     5701 ctgacctcgc atcatctggc gttatgttct ccattgatac cgaatccggc tttgaccagg
     5761 tggtgtttat cacttccgca tggggccttg gggaaatggt cgtgcagggt gcggttaacc
     5821 cggatgagtt ttacgtccat aaaccgacac tggcggcgaa tcgcccggct atcgtgcgcc
     5881 gcaccatggg gtcgaaaaaa atccgcatgg tttacgcgcc gacccaggag cacggcaagc
     5941 aggttaaaat cgaagacgta ccgcaggaac agcgtgacat cttctcgctg accaacgaag
     6001 aagtgcagga actggcaaaa caggccgtac aaattgagaa acactacggt cgcccaatgg
     6061 atattgagtg ggcgaaagat ggccacaccg gtaaactgtt cattgtgcag gcgcgtccgg
     6121 aaaccgtgcg ctcacgcggt caggtcatgg agcgttatac gctgcattca cagggtaaga
     6181 ttatcgccga aggccgtgct atcggtcatc gcatcggtgc gggtccggtg aaagtcatcc
     6241 atgacatcag cgaaatgaac cgcatcgaac ctggtgacgt gctggtcact gacatgaccg
     6301 acccggactg ggaaccgatc atgaagaaag catctgccat cgtcaccaac cgtggcggtc
     6361 gtacctgtca cgcggcgatc atcgctcgtg aactgggtat tccggcggta gtgggctgtg
     6421 gtgatgcaac agaacggatg aaagacggtg agaacgtcac tgtttcttgt gccgaaggtg
     6481 ataccggtta cgtctatgcg gagttgctgg aatttagcgt gaaaagctcc agcgtagaaa
     6541 ccatgccgga tctgccgttg aaagtgatga tgaacgtcgg taacccggat cgagctttcg
     6601 acttcgcctg tctgccgaac gaaggcgtgg gccttgcgcg tctggaattt atcatcaacc
     6661 gtatgattgg cgtccaccca cgcgcactgc ttgagtttga cgatcaggaa ccgcagttgc
     6721 aaaacgaaat ccgcgagatg atgaaaggtt ttgattctcc gcgtgaattt tacgttggtc
     6781 gtctgactga agggatcgcg acgctgggtg ccgcgtttta tccgaagcgc gtcattgtcc
     6841 gtctctctga ttttaaatcg aacgaatatg ccaacctggt cggtggtgag cgttacgagc
     6901 cagatgaaga gaacccgatg ctcggcttcc gtggcgcggg gcgctatgtt tccgacagct
     6961 tccgcgactg tttcgcgctg gagtgtgaag cagtgaaacg tgtgcgcaac gatatggggc
     7021 tgaccaacgt tgagatcatg atcccgttcg tgcgtaccgt agatcaggcg aaagcggtgg
     7081 ttgaggaact ggcgcgtcag gggctgaagc gtggtgagaa cgggctgaaa atcatcatga
     7141 tgtgtgaaat cccgtccaac gccttgctgg ccgagcagtt cctcgaatat ttcgacggct
     7201 tctcaattgg ctcaaacgat atgacgcagc tggcgctcgg tctggaccgt gactccggcg
     7261 tggtgtctga attgttcgat gagcgcaacg atgcggtgaa agcactgctg tcgatggcga
     7321 ttcgtgccgc gaagaaacag ggcaaatatg tcgggatttg cggtcagggt ccgtccgacc
     7381 acgaagactt tgccgcatgg ttgatggaag aggggatcga tagcctgtct ctgaacccgg
     7441 acaccgtggt gcaaacctgg ttaagcctgg ctgaactgaa gaaataaaat aaatccccgg
     7501 cggcgttttg tcgccgggtt tatgtgatcc ccgaagatga aacttattca atctcttcac
     7561 agacatcctg agttaatcgc cgcataatat ctttccttaa caaaaacttt tgtattttac
     7621 ctgaggcagt gcgcggtagt ttttcgatta ccacgatatg ttcaggatat ttatattttg
     7681 cgacccgttt acggctaaaa aaagccacca cctcttccag cgataatgaa tgatgcggcg
     7741 ctttcagcac gacataagcg catgatcgtt cacctaaacg ttcatcgggc attgcaacca
     7801 cacaggcatc gtgaatttta ggatgctgca ataaaatatc ttccacttca cggctgctaa
     7861 tattttcgcc gccgcggaca ataatatctt ttttgcgtcc ggtaattttt atatagccag
     7921 cctcatccat acggcagaga tcgccgctgt aataccagcc ttcttcatcc agcgcacggg
     7981 cggttaattc aggttcatca aaatatccca taaacacatt ggggccacgc gaggcttctt
     8041 caccttcgca acctggcggt aaggtcttgc gtgcgtcatc gaccacttta atctctacac
     8101 ctgcggcagc gtaaccatcg gtgtgcataa agcgcgacaa aggatcatcg agattcacca
     8161 ccgcatgcgg cgaactttct gtggaaccat aaacacttaa taatttaatg ccgcgctgct
     8221 ggcattcacg cgccactttt ttggggattg tggtaccgcc gcaaagaaag aagcgcagcg
     8281 ctgaaaggtc tgcgggttgt ttctctagta aattcaaaag atcatagaca aacggcgttg
     8341 cgccgagcat acaggtgcaa cgctgctgct caagcagcgc gagacacgca tcaggagtga
     8401 aaatatctaa caacacgctg cgagcaccaa tcaaaaatgg tgctgttacg ccatgcagaa
     8461 agcccgttgc gtggccgagt ggcgcaggca tcataaagac atcctgccag gtcagattca
     8521 gccgcgcgca ataagcccgc tcactggcga gaatattgtt atgcgttagc atcacgccct
     8581 ttggcagacc ctcggttccg gaggtaaaca gcaccgcagc taattcatcg ccgtgggtcg
     8641 ttatcgccgt ggtcagtggg gtattgtcgg cgataatctg acttaatgag agggaagagg
     8701 tggcgggagc cagtttgtcc acgccgacaa tttgttgtag ttgtggaagc tgattttgca
     8761 gcggcaggat taaatctacc ggacgcgttt gtttaaacaa cgtcggtgca aagaacattt
     8821 ttgcctgaca cttattgagc acccatacca gttctgcttc ccgccaggaa ggcaacagcg
     8881 gtacggaaac cgcaccgatt ttcaggcatg caagatagat aacagtaaat tcacaccagc
     8941 caggcagttg aaatgcgatg cgatcgcctg attcaatacc cttcgctaac atccagtttg
     9001 ccagacagct cgcggcgtga tcgagcgcgc tataggtgta cgatgctcca tgattatcga
     9061 ccacggcaat tttgtctggc atcgcacgag cggtctgctg ccagtaatcg gccagcgaag
     9121 catcgcccca tagcccttgc tgacgatacg ccgcacgacg ttgttcgttg aacgttaatg
     9181 tcactttcat gttggactct cgaaacagaa catcaggccc gagatgcggg cctgtgggat
     9241 gcatgattag ccgtagcgaa aatcaatacc gaaggtgcct gcgggatatt gccactgttc
     9301 gagaatagtg ttaccgcaca gcacccgaca ggtgccgcac tccagacaac cggcagaatc
     9361 aaaatgaatg ttgccggcgt catcctgctt ataaagtccg gcagggcagg ctttcattaa
     9421 tttacggaat tcattgatat cgggatttgc cgccagaatg atatgcgggt ggccctcatc
     9481 aacatggaat ttattgacgc ctaatttgat gtcgacgtta accgtagcgt tctggctcat
     9541 agcgcggttg ctcccttaat gccatctttc agcaagttga tcagcccaat tttcttcgca
     9601 tgtcccatga tcattttgcg taccggctgg tttggtttgc cgtcaatggt gaacatctcg
     9661 ttcatgatgt cggcgaccat tcgtgggtat tggctaaaca ggcgcgggtt ttctatcagc
     9721 gccgggatct tgcggaaatg ctgcatatcg cgcatgacgc agctttgttc cagttcacgt
     9781 ttgtattgcg ccagactgct ggcggagaaa tcctcgcgtt ctttggcggc gatcaccgtt
     9841 gtggcggcag cctgagccga tgcaatggct aaatccatgc cacgaactgt aaaacccaaa
     9901 ttcaggcaga agcctgcggc gtcaccaacg atcatcacgc catcgttaac catctgcggc
     9961 accattgcca gaccgccttc tggcaccata tgcgcggaat attcaagcag tttgccgccg
    10021 ctaattagtg ggcgaatggc ggggtgttgt ttaaaatctt ccagcatttg cggcacgctt
    10081 ttttgcgcat gggcgatatc acccaatcca caaaccagcc ccaaggatat ggaatccttg
    10141 ttggtataga gaaatccccc gcccatcagg ccgtcagaag gggaaccggc aaacagccag
    10201 gcggcacctt cctcgcccgt aatattaaag cgatcgttga tctgttctgg tgtgaggcca
    10261 ataacctctt taacaccaac agcgtaatga tgcggatcgg aagcgggaac cattcccagc
    10321 gagcggccaa gcatcgagtt aacgccatca gccagaatca ccacattcgc ttcgagaata
    10381 tcatccccag cctgcacgcc agtgaccttg tttccttcac gaaccagcgc atcgacgcga
    10441 acgcctggga taaactgcgc gcccgcctgc tcggcttgtt ccatcaacca cgggtccaga
    10501 cgattacgca atacggtata agatgcgtgt tgcggaacat ctggttgctc gcggtgaaaa
    10561 tcgagggtaa cggcgctttc ttcggttaag aaggagattt tctcgcgtgt gaccttgcgt
    10621 tctaccggcg ctgatactgc aaagcctgga atgattgctt caagtgtgtg ggcataaaga
    10681 cgcccgccgg tcatgttttt acatccggca ctgtcgccgc gttctatcac caggacatcc
    10741 agcccggctc gcgccatgac cagtgcggca acgctaccag caacgcccgc accgaccaca
    10801 atggcatcaa atttgtcttc cgacatgcgt tatgctccct tcccgctgcg tttacagcgg
    10861 gagatgtggt tgaaagggga attagcggct caactggcta atcagggcag ggacgacttt
    10921 gtagatatcg cccaccagac cgtagtcggc ataattgaag attggcgcat ttttatcttt
    10981 attgatggcg acaatcactt ttgcgccgtt gccgccaacc atatgctgga tctgcccgga
    11041 gatccccagc gtcaggtaga gatcggattt cagcaacacg ccggagacac cgatataacg
    11101 ttcacgctcc atccagttct cgccttcggc aattggacgt gaacagccga cttcagcatt
    11161 cagcaccgcc gccagttcgt ggaccatttt caggtcatcc tgcgccgcca gaccacgacc
    11221 gacgccaacc acacgttttg ctttgctcag gtccacgctg cttgcggctt tcgcacggcg
    11281 ttcgcgacag agaatttcat gacgcggagc aacataaggt accgtttctg tcgggcactg
    11341 atgagaggta tcactggtgc acggttcctg aacaccgggt gcaagggtaa tgatcgccag
    11401 cgggctgttg atcttttcct gagcgaacgc taacccgcca tacatccggt gttcggcgca
    11461 aatgtgacca tcgacaatat ccaccgccgt ggcatcgttc accagcgccg cattcagttg
    11521 cacacttaac cgtgctgcca gagctttacc acgtttcgtc gcggccaaca gcaacatagc
    11581 ggggtgttta tctttcagca gggcagcaat gctttcggcg taattttcag tgcgttgcag
    11641 cgcgtcgttt tgctcaagaa cataaataca ttttggacca taaggcataa ctgcctgcgc
    11701 ctggtcggta ttttgtacaa tggcatacac ctgttggccc cattgctgag cgccgccaaa
    11761 cagttcagca taacgttcag gattatcgct aaagacccag acgctgttta attgactcat
    11821 cgtttcttct ccgtaggctt cagttcaggg ctttcttcag atgttccgcc agctcggcaa
    11881 tggcctccgg agaatcgtta tcgataatga tgtgcttacg ttctgtttgc ggcggtacgc
    11941 gaatgccgac cagttcagca agtggcgcgc tctggctcca gtcaatatca cttgcctgcc
    12001 actgatttac cggtttttta cccgcgccga gaatggcttt catcgaagga atgcgtggca
    12061 cgttaatatc ggaggtgacg cagagcacgg ctggaacaga gagttcaata acttcaacat
    12121 catcttcaag cgtgcgttca atcaccagtg tattgccctg acgctgaata gcactcactg
    12181 cattaatcac cggaagttgc agaatttctc cgaccagcaa gccaacctgc tgggcataaa
    12241 ggtcgccgga accttcacca aagatcagta aatcgaagcc gatcttttca attgctgccg
    12301 ccagcgcttt tgcggtatcg agaggcagtg catgttcaag ttgcgcatcc tgcaccaaat
    12361 acaggctgtg cggcccgcgg gatagcacgt ctttgcgcac tttcgagttc tgcaacaatg
    12421 agccgccaac ggtcagcgcg gctatctcat cgtcatctgt tgcgagctgg cttgcagctt
    12481 caatggcatt gagatcgaac tggctgattt tggcgtcggc attgtcgaaa ttcagggtgt
    12541 attctggagt gacaacaatg tcctgttctt caggcaccag cttaaagcag gttattattt
    12601 tcatgatttc tcctgtgagt cgcatacgga accatctccg atggaaggta aaaatggcct
    12661 gaaaatagtg tgcgatagcg gtgcggggtt ttcgacggag caagctgttt tccttgccaa
    12721 caatcttgca aaaaaaatgc gatcgctaaa acaaaagtgc aatttgcggc aatatagtta
    12781 ttccatgacc gtacaatctt gcattcagtg taaataatct tgtttagctc tcgctaaacg
    12841 cggttgtata accgcaatac acgccgtatt ttatccggca tatattgttt ttgcttgctc
    12901 atggaatagg gaggttgtca tgtatcaacg ctgttttgat aacgccagtg aaacgctgtt
    12961 tgtcgccggt aaaacgccac ggctttcacg ttttgcattt agcgatgatc caaaatggga
    13021 gtccggacat cacgttcatg acaatgaaac cgagttgatt tacgtcaaga aaggggttgc
    13081 aaggtttacc atcgattctt cgttatatgt cgcacatgcg gatgacattg tggtgataga
    13141 acgcggcagg ctgcatgcgg tggcctctga cgttaacgat ccggcaacga cgtgtacctg
    13201 tgcgctgtac ggctttcagt ttcagggggc tgaggaaaat cagctactgc aaccgcattc
    13261 ttgtccggta attgccgcgg ggcagggaaa agaagtcata aaaaccttat ttaatgagct
    13321 aagtgtgatt ttgccgcaaa gtaaaaatag ccaaacatct tcgttatggg atgcatttgc
    13381 ctatacgtta gccattcttt actacgaaaa ttttaaaaat gcttatcgtt cggagcaggg
    13441 gtatattaaa aaagatgttc tgataaaaga tattcttttc tatctgaata ataattatcg
    13501 cgaaaaaatc actttagaac agttatcgaa aaaatttcgt gccagcgtca gttatatttg
    13561 ccatgaattt accaaagagt atcgtatttc ccctattaac tatgttattc aacggcgtat
    13621 gacggaagca aaatggtcac tcactaatac tgaattatca caggcagaga tctcctgacg
    13681 tgtgggttat gaaaatgtcg atcactttgc caaactgttt ttgcgccatg tcggttgttc
    13741 gcccagcgat taccgtagac aatttaaaaa ctgttttgcg gaacaagaaa tcctatctga
    13801 atttcctcaa ccggtaagtc ttgttggata aatctggcgg cgcagtttgt taagaagctg
    13861 cgccgcctgc agattatttg ttctgatagt ctttcaggat ctgccgacct gctacgtaaa
    13921 tcataatttc gtctgtaccg ccgccgatac gttcacaacg gacatcacgc cagaagcggg
    13981 agacgcgcgc ttcatcggta tagcccagac cgcccatgat ttgaatcgca tcatcaatga
    14041 cttccattgc ggtacgtgcg caatacagtt ttgccagcgc cgcgctggtg cgcagtgact
    14101 gatgctgatc ggcttgccat gccactttca gcaccatgtt gcgcatgttg tcgatcttaa
    14161 tcgccatcag cgccagtttt tcctggatca tctggttatg accaatgggc ttaccaaaag
    14221 cgatacgttg gttggcatag cgggcggcat cttcaaaggt gcattcggca aagccggtgc
    14281 tgcgcgcggc gttgatcagg cgctccatct caaagttgta catcacattg aggaaaccca
    14341 ttccttcttc gcccaccatg tcgctctctt caacctccac gttgtcgaga tagacttcgc
    14401 aggtgctgag catatgccag ccgattttat gcagcgggtt aatcttaatg ccgggcttac
    14461 tggagtcgac ccaccacagg gtgaaggctt ttttgggatc tttcggttgc ggatcgcgcg
    14521 ccaacaccag catatacggg tactctttcg caccggtaat aaaggttttt tgtccgttga
    14581 tgtagacctt gccgttttta cgcgtgtaag tggtagtggc actgttgtta tctgagccag
    14641 cacctggttc cgtcaacgcc agggcatagg cgggatcacc cgtttccagg gggctttctg
    14701 ccgttttacg tagctgctct gcagaaccga aacggcgcat actgtgaata cattgaccgt
    14761 tggtgatcaa aaatgccgga gcaccgcatt ttgacacttc catcagcgcc agcatttggg
    14821 tgacgtaatc cgcagggata ccaccaaatt cttccggcac gccaagcatg gaaataccgt
    14881 tatccgccag cgcccgcata aactcacgcg gatatgtccc gttttgatcg caggtgcgga
    14941 aatactcttc cggaaagtta gtcgtaatca gttcgcgaat actggccagc agcagttctt
    15001 gttcttcagt taaagaaaaa tccatcatta tactcctgtt aatgagccgc ttcaggcagg
    15061 acaaaaccca tcgccgcatc gataaataat ctttcgttca tcagtttgag ttctggcgaa
    15121 atcactgggg tgaagtccat tttgtcgaga atatcttttt gtaaatcgac gccaggggcg
    15181 atttcgatta aatgcaggcc gtcttctttc agcgtgaata cagcacgttc ggtgatgtaa
    15241 cgaacatcca gtccccgttc gagcgcgatt tttccgctaa aagtaatttc cggtagttcc
    15301 cgaataaatt tatttacccg tccttcctgg acgatatgca atttgccatc ggcaatttct
    15361 gtttttaaac tgcccgcagt taacgtgccg cagaaaataa ttttcttcga ggtggcactg
    15421 atatcaataa atccaccggt acccatgatt ttgccattga atttatgcac gccgacgtta
    15481 ccgtgctggt cgacttcagc aaaactcaaa taacaaacat ccagaccacc accgtgataa
    15541 aaatcaaact gggacgtcat atccagaatg gcgcgggtat tcacgttcgc gccaaaggcg
    15601 ataccctgtg aagtaattcc gccaatcgga cctgtttcta ccgtcagaat aaagtcatca
    15661 gcacaacctt cttcccgggc gaccaggcca ataccgtcag caataccgac gccgacattc
    15721 cccaccgcgc ctttacgcat ttcgaataat gcacgccgcg caactaattt acgttggttt
    15781 aggggcaggc taagtttggt gctgtcatca agggtgaagt caccagaaat aaaacggtta
    15841 accggcgcac cgccatacag ttggctttga tccggatcga ccaccacaat atccaccaga
    15901 taacccggaa tacggacaga tttaggatgc agcgtggctt tcttaaccat tttctgcacc
    15961 tgcatcatca caataccgcc gttattgtgc accgcctggg caataaccaa tgcgtcgaga
    16021 tacatcacct catcttcaaa agtggcgtag ccttcactgt cgcaggtggt agcgcgaatg
    16081 aaggcgatat ctggcgcaat cgctttgtaa tagagatatt ctttgttatc aaactcgacc
    16141 agtttaatca ggtcttcttt cgtgacttca ttcagtttgc cgccttgctg gcgtggatcg
    16201 acaaatgtcc cgatgccaat atcactaata ataccaggct ggtgggctgc ggcggcgcgt
    16261 aaggtttgtg taagtacacc ttgtggatag ttataagcaa taattttatt ttgttctgcg
    16321 agttcagaaa tacgcggcga ttgtccccag tgaccacata atgcccattt caccagacct
    16381 tcttgcgcca gaggactaat accacggtcg gcgcgatcgc caagccctgt cggactaata
    16441 atcgataaat tacgtggcgt ttgagtctgt ttatatttat cagcaagagc tgtaattaac
    16501 gtggtggctt ccagaatacc gccgccagcg cctaacacac aaagtgttgc ttcgtcggga
    16561 atataattca ccgcttcctg tgccgacagg accggcactc gtccattaat acgaggtggt
    16621 tttacaggtt tcattgttaa ttcctgattc agatgtgatt ttccctacgc cagatggaga
    16681 attagcgcac agagactcac gaaatatttt tattaaatac ggttaaatat attttttaag
    16741 ttcggcgggg agggtgttcc cgccgaaata ttattgctta tgcctggtgt aaaatagtta
    16801 ataccgtgcg taaatcattt accgagattt gccctggtgc agaggctttt tttaccgcac
    16861 caaaagttgc cgccgagcca aatacttcac cagccagacg agaaattacg ccagtttttg
    16921 ccatcgacat cgtgatgatt ggacgatcgg catactgctc ctgcatctcc agggtcgcgg
    16981 taagcaacgt cagcacatcg ctggtacttt gcggcatcag cgcaatctta ggaatatcgg
    17041 cgtcgaagga ctgcattttg cgcagacggg caatgatttc ttcggcttcc ggcgttttat
    17101 ggaagtcatg gttggacatg acaactttca catcatgcgc gtgggcgtag gcgacggttt
    17161 ctttaacctg atcatcgccg gtaaataact ccagatcgat catatcaacc aggccgctgt
    17221 cgatggctgc acgattgaga gcaatataag cctcggtgga aatcgcctgc tcgccgcctt
    17281 ctttggcact gcggaaggta aacagcagcg gtttttctgg catggtttca cggagaattt
    17341 ttgccgccgc catgacagac tccacattgg agaggtcggc aaagtggtcc acacgccatt
    17401 ccagaatatc aaagtccgct tcacgatagg cgagagcttc ggatttcacg cgggcgatat
    17461 ctttcgccat cagcgagacg atgattttag gtgcgcccgt accaatgacg agatctttta
    17521 cagttacggt tttcattttt taccctttct gcacgcggtc agcctgtcag gcaccgaacc
    17581 ccatgacctg tttaacatat tccagaggga aatctttgcc agtccataac gtgaactgtt
    17641 cagccccttg ccacaacaac atgccgtatc catcaatcgt tttgcaacca gcttgttgcg
    17701 cctgctgcaa taacttcgtc atatgcgggt tatacacgca ttcagtgacc agcagtcccg
    17761 gatgtaacag actgatatca ttaactaatg attcattctc aaggggtttc atacccactt
    17821 ttgtgccatt ggttaaaatg tcggcggaag ccagggcttc agcaaaggct tgctgatcgg
    17881 cgagatcggt aaccgtgacg acacaatcgg tgttttcgtt aacccgctgc gcgaaggcga
    17941 gggctttatc gaagaactca tcccgacggt taaagagttt aatttctttt aaaccttcaa
    18001 ttgccccctg cgcgccaatc gccgttgagg cacctccggc ccctaacagc accattgttt
    18061 tgcctttgat atcaaaaccg ctctctttaa tggcgcgaat atggcccgtg ccgtcggtgt
    18121 tatagccgcg caggtagcca tcatcattaa cgatggtgtt gatggcaccc accagtttgg
    18181 ctgctggcgt taattcatca acatattcac acgccagttg tttgttcggc attgatatac
    18241 cagttccgcg cattttgagg gcttttaatc cttcaattgc tccgggaaaa ctatcgttat
    18301 ccacttcaaa ggccatatag gtaaatggca atcccgcttt ttctaaggct ttattctgca
    18361 tttcaggcga taaactgtgg cggataggat aggccatcag cccaatcaat tcgtattttg
    18421 cggtaacatc cataattaac tcctttaacc tctatgctta attgtgttta accgggtgct
    18481 aaatttacgc tcgccaaacc gcacatcatt ctcaggaata ataaaaacgc ggtaatagcg
    18541 gataaaaaca ataattgcgg taatcagggc cagcagcgcg aaagtaaaat cgagaacaat
    18601 gatatattga aggccgatgt tcgacagata accggtaatc agcggaataa caaagttagc
    18661 cagtccaccc atcatcatat aaatactggt gactttggct ttgctttttg ggaaaaactc
    18721 tgacatgacc gaaacaccga gctgtaaaat gccgccagct gctgagaaac cgataacaaa
    18781 ggcgccggca ttacacacca gtggagaagg gtaccggtag ataatggctg ctgttattgt
    18841 tgccagtgca gagttaaata cattagccca gatgggccgg atcatttttt tcagtagtgc
    18901 ggcaaaaata aagacacaga ccaacgagcc catactgtaa taagagatgg tttttaatgc
    18961 ctcagcttct gacataccag caaaagccat cgcatatttg ggcatccaca ccacaatcac
    19021 ataaaaggtc gagaatgcgg ctacaccgaa cagtaccgat gaaacacctt ccagccagac
    19081 taacggtttg ctgttcattt gcggtaattc attagctacg ctggcgtcca ccaactggct
    19141 ggggaatttg cttttcaaca gcatcagcgt gatcagtaca aacagaatac ccggaataat
    19201 cagcccatag ccgtaccaga tattattgag caacatatag ctcaccagca ttgggtagaa
    19261 catttgccca aatgacacca tcgctttaac cagtatgacc gccgaaccag aggctttcgg
    19321 aaagcattcc atgagcgcgg ggtagccacc cgtatccagc gctgagttag cgatacctac
    19381 gcacactgct agaccgtagg cgagagttaa attcgggcaa gcgggaatac caaagaagaa
    19441 tagcagatac attattactg ccattaatat caccgcccga cgaccaaact tatcggagat
    19501 cacaccgaag aataaaatac tgatcaatcg ccccaaaccg ataccggaaa ttaagtaggc
    19561 aatgcccgcg ttgtcagtgg aaaacttttc tgccagagat gacatatttt gggcaagcgt
    19621 aataacacta atgccgtgca ggaaatagct gaagtaaata caaagaacag ccaggataaa
    19681 tggcgtgctg aaagccttat tttgagacat tttcacagct ccttgctgaa agataatttc
    19741 gttaccgtac gtattgttct ccgacggttt acataattac tatactgaag atgattttcc
    19801 ctgggtacat ggcataaagt ttatgtttcc aggaatattg catgtcatag tttgtttttg
    19861 atcgcgattt tccttagtta gcgcagaaag agttatttat ccgtaagatc ttgcatgacc
    19921 tgaaaagaat attgcattac ccacccggag cgacattttc ctttagcgag tgatgccgcg
    19981 tttttttgcg gctgcgtaag ccgataaaca gtgccagtaa aaagccgatg gcagcgatgg
    20041 cggtatcgaa ccacataata tcggcaatac ttctttggga cagatgagcc gtaatcaacg
    20101 gaatagtaaa ggtcgcaata ctgcccgcac tgtaatagat ccctgtagct ttacctttag
    20161 cgtaggggaa acgttcagcc attaacgtca ggccaatttg cacaacacct ccagcagagg
    20221 taaaaccaat gacaaaagca aatattatca ccacataaaa tgtgggatgc aggcagacgg
    20281 taaacagagc aataaatgag ataaaggtgt acagcatcag taatgttgtt gggcgaacgg
    20341 tatttcgaat gagtggagcg gtaataaata cacaaagcag cgaacccacg gtgtagatac
    20401 tgagtagttt gatcgacata gtgtatgaca tgcctgcaac aaattgtccg tactgtgaca
    20461 gccactggct aaccagataa aacgttgcca ttgagatata gccatataag gtataactgg
    20521 ctaaatcgat aattgaacag cgatgttccg tagagcgggt ggttttcttt atgacaggta
    20581 agcgacgacc cggatggggt gggaacgtac aacggtataa aaacagagcg ttaataagca
    20641 taatgcctgc agcaatcata aaggaccaac cgaaccacag ttcagcccac actaacaggc
    20701 taatgattag cggtaataaa aattgtccgc tggaaacaaa tgctttaatt aaaatattgg
    20761 ctgtcccagg tgagcgtgga aaagcttcca tcaaactggg ataagtgcct gcatcgagaa
    20821 aactgtttgc cattcccgcc agaaagccaa aaacataagc gataatgatg ttattggtct
    20881 gcaggatgcc aaaaaagaag gccatatagc aacacatccc gagcatgata aaagggcggc
    20941 gaccaaagcg atcggataat aatcctgcaa aaagcaagac acttaatcga ccaatgccca
    21001 gcgatgagat aactatcgag acacccgcgg cattagtctg ccaaagtgtc tccagcgagg
    21061 ccatattcag gctcatcaaa atgacgccca taccatgcac caggtaatta aaatacaacc
    21121 caagtgcggt agggtaatag ggatttttca taggttctac ttagcaaagg tgctttaaaa
    21181 agtgtagcga tgttgtgtca gtatccattc attattgttg taacaacgac aattcctttc
    21241 tgtggaaaaa ttagcaaagc cgctccagat cgtgtgcata taattcagcg gctaccgatt
    21301 gatagatctt ccagtcaata aaattgccat cagggtagac tcgttgaaat gcggttaagt
    21361 cggggaaaaa acgcatggtg ttgttgccga tgcggttatt tattttctct atgatggcat
    21421 gaagatgttt ttttctttgc tgacgcatag ccaacagttg ctcgactaca cgatcaggaa
    21481 tggcacactt gccattttcc cattgacgcc agctttcact gtcaccggtc tgggcaatcc
    21541 aggtcgcgca ttcatcaata gtcatggcaa aaatatggcg gagtgcctgg agttcataag
    21601 cgttcatcgt gtaatggctg catgaaataa acaataaaat ataaataaca ttatcagcct
    21661 gctgacggct tgaagagagt cacagtatct tgtgcaacat tattggaaag agtgaggggc
    21721 gaggtagcga agcggaaaaa tgttaaaaaa aagcccatcg tgggagatgg gcaaagacta
    21781 cacacagcaa ttcgttgttt cactcagggg atttccatgc ttataaatca atatgttgat
    21841 ttataaccgt gagctaatag taggcatacg gactttttgc gtcgatcaga ttcgtctcat
    21901 tagtttaaaa actgtaaaga atttgcgaca ggtactacca gctgaatggc ggtaagcagg
    21961 aaaatatggg gaagagcaag caaatatcaa ttagcggtgg ttaacaatca ggcggcagag
    22021 ccgcctgatg agaaattact tattcggttt ctcgatttcg ccaagttctt cgagaatttc
    22081 ttccggttgg tccgtcggcg gcgggacttc ttccacccac gcggcaaaca gacgccagga
    22141 aacggctaac agaaccggac caataaacag accgatcatc ccgaaagcaa tcaaaccacc
    22201 aataacgcca gagaggatca ggatcagcgg taaatcggca cccatgcgaa ttaacattgg
    22261 gcggatgacg ttatccagcg tgccaaccac accgctccac actaacaata ccgttcccca
    22321 ggtggtatcg ccggtccagt agagccagat aatcgccgga atcagtaccg gcaacgggcc
    22381 aagctggaca aggcaggaga ggatcattaa caccgttagc aacgttgcat aaggtacgcc
    22441 ggatacggcg aggccgatac cgccaagcac tgcctgtact aacgccgtca ccaccacacc
    22501 cagcgccacc gcgcggatag cctgtgccgc cagcagcacg gcggcatctc cgcgaacgcc
    22561 tgccagacgg gttgcaaaat ggcgaatgcc ttgtgccacc tgttcaccgc gccagtacag
    22621 cagggcactg aagagaagca tcagcgcaca atgcaccata aagcgcccga tatgcgccgc
    22681 ctgcccaacg aaccaggtgg tggtggtgcc aatataaggg cggactttcg ccatgatcgc
    22741 cgtccccccc atatccagca agttgtgcca gcctgcatac agcttcgcgc caatcaccgg
    22801 aatggtatta agccacgcca gatcgggtaa cgtcatgtcg ccgctggaaa tggctttaat
    22861 tagcgggccg ctgccgtcga cgatactgtt aaccagcaaa gcaataggga tgataaacac
    22921 catcactaat aacagcgtca tcaccagaac ggcgagagag cggcggccaa acatgatctt
    22981 ttgcaaacgt aacaataccg gccaggtggc gataaccacc gtaccggccc atgcaaagcc
    23041 gagaataaag ggttgaacaa tccacagaca tgccacaatc atgatggcta aaaacagcac
    23101 cgaaagcaga atttgtgcga catccctggg ctgacgaaca tttaccataa ctacttttca
    23161 ccttttttgt gcgtcaaatc gtcgacgcga cgtgaacacg cgaaactcac ctgcataatg
    23221 atgagcaatt tcagcggttt ttaacaggcc gattctgccc ttaattctga tgggcgtgtt
    23281 agaaaaaaat gtggtacaac aactcaggca acacgcaaac gatttactcg tcgtatttca
    23341 agttgcatgt gctgcggcta cgttcgttca ccccagtcac ttactattgt aagctcctgg
    23401 agttcactcg cttgccgcct tcctgcaact cgaaatccat agagtaaata aacatcacct
    23461 ttcagtgcgc tattgcagcc gcctgaaaga atagtgtatc gacctacatc acagaccgca
    23521 ggaaagggtc aatataatga ttccacagat ttcccaggca cccggcgtcg ttcaactggt
    23581 gcttaatttt ttgcaagagc tggagcaaca aggttttacc ggcgatacgg cgacaagtta
    23641 tgccgatcgt ctgacaatgt cgaccgacaa cagtatttac caacttctcc ccgatgcggt
    23701 ggtatttccg cgttcaaccg cagatgtggc gctgatcgcc cgtcttgccg cgcaggaacg
    23761 ctattcatcg ctgatcttta ccccccgcgg cggcggcacc ggcactaacg gtcaggcgct
    23821 caaccagggg attattgttg atatgtcccg ccatatgaac cgcatcatcg aaattaaccc
    23881 tgaagagggc tgggtgcgcg ttgaggccgg ggtgataaaa gatcaactca atcagtacct
    23941 gaaaccgttc ggctactttt ttgcgccgga actttcgacc agcaaccggg caacgctcgg
    24001 tgggatgatc aataccgatg catccggtca gggatcgctg gtctatggca aaacgtcgga
    24061 tcacgtactt ggcgtacgcg cggtgttgtt ggggggcgat attctcgata cgcaaccttt
    24121 acccgtcgaa ctggcggaaa cgctgggtaa atccaatacc acaatcgggc gaatttataa
    24181 cacggtttat caacgttgcc gtcagcaacg ccagttaatc atcgacaact tccccaaact
    24241 taaccgcttt cttaccggtt acgatctgcg tcatgtcttt aacgatgaga tgaccgagtt
    24301 cgacctgacg cgcattctga cgggttcaga agggacgctg gcctttatta ccgaagcgcg
    24361 gctggatatt acgcccttgc ctaaagtgcg ccgtctggtg aacgtcaaat atgactcttt
    24421 tgactccgcg ctgcgtaacg cgccgtttat ggttgaggcg cgggcgcttt cggtagagac
    24481 ggtggactca aaagtgctga atctggcgcg ggaagatatt gtctggcatt ccgtcagcga
    24541 gttgattacc gatgtgcctg acaaagagat gctcgggctg aacattgtgg agtttgctgg
    24601 tgatgatgag gcgctgattg acgagcgagt taatgctctc tgtgtgcggc ttgatgagct
    24661 gattgccagc caacaggcag gtgtgattgg ctggcaggtg tgccgcgagc tggcgggtgt
    24721 tgaacgtatc tatgcgatgc gtaaaaaagc cgttggtctg cttggcaatg ccaaaggtgc
    24781 cgctaagcca attccgtttg ctgaggatac ctgcgtaccg ccggaacatc tggccgatta
    24841 tattgctgaa tttcgcgcgc tgctcgacag ccacggctta agctacggta tgttcggtca
    24901 cgtcgacgca ggtgtcttgc acgtccgtcc agcgctggat atgtgcgatc ctcaacaaga
    24961 gattttgatg aagcaaatct ctgatgacgt agtggcgctg actgcgaaat acggtggttt
    25021 gttgtggggc gagcacggca aaggttttcg cgctgaatac agcccggcgt ttttcggtga
    25081 ggaacttttt gcagaactgc gcaaagtgaa agcggcattt gacccgcata accgactcaa
    25141 cccagggaag atttgcccgc cagaaggtct cgatgcgccg atgatgaaag tggacgcggt
    25201 gaagcgcggt acattcgatc ggcagatccc cattgcggta cgccagcagt ggcgcggtgc
    25261 gatggagtgt aacggcaacg gtttatgctt caactttgat gcccgtagtc cgatgtgtcc
    25321 gtcgatgaag atcacccaga accggattca ttcaccgaaa gggcgcgcaa cgctggtgcg
    25381 tgaatggctg cgtttgttgg cggatcgcgg cgttgatcca ctcaaactgg aacaagaact
    25441 gcctgaatcc ggcgtcagtt tgcggacgtt aattgcccgc acgcgcaata gctggcatgc
    25501 gaataaaggc gaatatgact tctcacacga agtcaaagag gcgatgtcgg gctgtctggc
    25561 ctgtaaagcg tgttcgaccc agtgccccat caaaattgat gtgccggagt ttcgctctcg
    25621 ttttctgcag ctctatcaca cccgttattt acgcccgctg cgcgaccacc tcgtcgctac
    25681 ggtcgagagc tacgcgccgc tgatggcacg cgcgccgaaa acctttaact tcttcattaa
    25741 ccagccgctg gtgcgcaaac tctcggaaaa acatatcggc atggttgatt tgccgctgct
    25801 gtcggtcccc tcgctacaac aacaaatggt ggggcatcgc tcggcaaaca tgacgctgga
    25861 acagcttgaa tctctcaatg cagagcagaa agcgcgcaca gtgttggtgg tgcaggaccc
    25921 ctttaccagc tattacgatg cgcaagtggt ggcggatttt gtccgtctgg tcgaaaaatt
    25981 aggtttccag cctgtgttac tgccattttc gccaaatggc aaagcccagc atattaaagg
    26041 ttttcttaat cgttttgcga agacggcgaa aaagacggcg gatttcctca accgtatggc
    26101 gaagctgggt atgccaatgg taggcgtcga tccggcgctg gtactttgtt atcgcgatga
    26161 atataaactg gcactgggcg aggagcgtgg cgagtttaac gtcttactgg cgaatgaatg
    26221 gctggcaagc gcacttgagt cacagccagt ggctacagtc agcggtgaat catggtattt
    26281 ctttggtcac tgtaccgaag ttaccgcctt gccgggtgcg ccagcacaat gggccgcgat
    26341 atttgcccgt tttggcgcga aactggaaaa tgtcagcgtg ggttgctgcg gcatggcagg
    26401 gacttacgga catgaagcga aaaaccatga aaattcgctc gggatctatg agttatcctg
    26461 gcatcaggct atgcagcgac tgccgcgtaa ccgctgtctg gcgaccggat attcctgccg
    26521 tagccaggta aaacgggttg aaggcacggg ggtacgccat cctgtgcagg ctttactgga
    26581 gattattaaa tgatatggaa acggaaaatc accctggaag cactgaatgc tatgggtgaa
    26641 ggaaacatgg tggggttcct ggatattcgc tttgaacata ttggtgatga cacccttgaa
    26701 gcgacaatgc cagtagactc gcggacaaag cagcctttcg ggttgctgca tggaggagca
    26761 tccgtggtac tggccgaaag tatcggttcc gttgccggtt atttatgtac cgaaggtgag
    26821 caaaaagtgg ttggtctgga aatcaatgct aaccacgtcc gctcggcacg agaagggcgg
    26881 gtgcgcgggg tatgcaaacc gttgcatctc ggttcgcgtc accaggtctg gcagattgaa
    26941 atcttcgatg agaaagggcg tttgtgctgt tcgtcacgat tgacgaccgc cattttgtga
    27001 cgttgtcacc agaaaagtgt gacgcatatt gcctttggtc attatctgca cagtaaacgg
    27061 tggtatactg tgcaggtctt gtctaaaagc gaggatgcta tgtctactca acttgatccc
    27121 acccagctgg ccattgaatt tttacgccgt gaccagagca atctttcacc tgcccaatat
    27181 ctcaaacgtc tgaaacaact agaacttgaa tttgctgacc tgcttactct ctcttctgct
    27241 gaattaaaag aagaaatcta ctttgcctgg cgtctgggcg tgcattgatc ctgacttaat
    27301 gccgcaagtt cgccaaatcg acctggatta acctccgtcc aggttcgatg accttcttaa
    27361 tactgcttat ctaccgcctg atattgttca aaaataatac taatcgcagt ttgtgttaaa
    27421 acggcgggtt agctttatga gattattctt atcgcccctt caagagctaa gccactgaga
    27481 gtgccggaga taagcgccgg atggggtaga aacccttaag cctgtgttgc acagacttaa
    27541 gggttttctt atttctgaac gctgcacttt tttacctatc tcttaaatag tcattttcaa
    27601 tacaactttt tctttttcat cacagcctta atgctcctta gataataacc attatctaac
    27661 aatgagatac ctaattctta gcgtgcctgt taacccacac atcagggtct atgcttatta
    27721 aaagcaggca aaaagcatgt tttacggtaa agcccctgcg tttgctgggt tgtactgata
    27781 atcattatca ctaacatgct gttatacgct gaaagcgatg aagtgaggta aatcgatgga
    27841 catgcattca ggaaccttta acccacaaga tttcgcctgg caaggcttaa cgctgacacc
    27901 cgcagcggcg atacacatcc gtgagctggt ggcaaagcag ccgggtatgg tcggcgtgcg
    27961 cttaggcgtg aagcaaacgg gctgcgcggg ctttggctat gtgctcgaca gtgttagcga
    28021 gccggacaaa gacgatctgc tgtttgaaca cgacggcgcg aagctgtttg tcccgctgca
    28081 agcgatgccg tttattgatg gcacggaagt cgatttcgtt cgtgaaggac ttaatcagat
    28141 attcaaattt cacaacccta aagcccagaa tgaatgtggc tgtggcgaaa gctttggggt
    28201 ataggcggta ctatgtctcg taatactgaa gcaactgacg atgtcaaaac ctggaccggc
    28261 ggcccgctga attataaaga aggattcttc acccagttag ccaccgatga gctggcaaag
    28321 gggataaacg aagaggtggt gcgcgcaatt tcggcgaagc gtaatgagcc ggagtggatg
    28381 ctgaagtttc gtctaaacgc ctatcgcgca tggctggaga tggaagaacc gcactggttg
    28441 aaagcgcact acgacaagct gaattatcag gattacagct actactcagc accatcgtgc
    28501 ggtaattgtg acgacacttg cgcgtctgaa cctggcgcgg tgcagcaaac tggcgcgaac
    28561 gcctttttaa gtaaagaggt ggaggcggcg tttgagcagt tgggcgttcc cgtgcgggaa
    28621 ggcaaagagg tggcggtgga tgccattttc gactcagttt cggttgccac tacttatcgc
    28681 gaaaaactgg cggagcaggg aattattttc tgttcctttg gtgaggcgat ccacgatcac
    28741 ccggaactgg tgcgtaaata tctcggcacc gtggtgccgg ggaatgacaa cttctttgcc
    28801 gcgcttaatg cggcggtagc ctctgacggt acgtttattt atgtgcctaa aggcgtgcgc
    28861 tgcccgatgg aactttccac ctattttcgc attaacgcgg aaaaaaccgg gcagtttgag
    28921 cgcaccattc tggtggccga cgaagacagc tacgtcagct acattgaagg ctgttccgct
    28981 ccggtgcgtg acagctatca gttacacgcg gcagtggtgg aagtcatcat ccataaaaac
    29041 gccgaggtga aatattccac ggtacaaaac tggttccctg gcgataacaa caccggcggt
    29101 attctcaact tcgtcaccaa gcgtgctttg tgcgaaggcg aaaacagcaa aatgtcatgg
    29161 acgcaatcag aaaccgggtc cgcgattacg tggaaatatc ccagctgcat tttgcgcggc
    29221 gataactcca ttggtgagtt ttactcagtg gcgctgacca gcggtcatca gcaagcggat
    29281 accggcacca agatgatcca catcggtaaa aacaccaaat cgaccattat ctcgaaaggg
    29341 atctctgccg gacatagtca gaacagttat cgcggcttag tgaaaatcat gccgacggca
    29401 accaatgcgc gcaatttcac tcagtgcgac tcaatgctga ttggcgctaa ttgtggggcg
    29461 cataccttcc cgtatgtcga atgtcgcaat aacagcgcac aactggagca cgaggcaacg
    29521 acatcacgta ttggtgaaga tcaactgttt tactgcctgc aacgcgggat cagcgaagaa
    29581 gacgccatct cgatgattgt taacggtttc tgcaaagacg tgttctcaga actgccgctg
    29641 gaatttgccg ttgaagcaca aaaactcctc gccatcagtc ttgaacacag cgtcggataa
    29701 ggaataaaca tgttaagtat taaagattta cacgtcagcg tggaagataa agctatcctg
    29761 cgtggattaa gcctcgccgt tcgtcccggc gaagttcacg ccattatggg gccaaacggt
    29821 tcgggcaaaa gtaccttatc ggcaacgctt gccgggcgcg aagattatga agtgacgggc
    29881 ggcacggttg agttcaaagg caaagatttg cttgcactgt cgccggaaga tcgtgcaggc
    29941 gaaggcatct ttatggcctt ccagtatccg gtggagattc caggcgtcag taaccagttt
    30001 ttcctgcaaa cggcacttaa tgcagtgcgc agctatcgcg gccaggaaac gctcgaccgc
    30061 tttgattttc aggatttgat ggaagagaaa atcgctctcc tgaagatgcc ggaagattta
    30121 ttaacccgtt cggtaaacgt tggtttttcc ggcggcgaga aaaagcgcaa cgatattttg
    30181 caaatggcgg tgctggaacc ggagttatgc attcttgatg agtcggactc cgggctggat
    30241 attgacgcat taaaagtggt cgccgatggc gtgaactcgc tgcgtgatgg caagcgctca
    30301 ttcatcattg ttacgcacta ccaacgcatt ctcgactaca tcaagcctga ttacgtccat
    30361 gtgctatatc agggacgaat tgtgaaatcc ggcgatttca cgttggtcaa acaactggag
    30421 gagcagggtt atggctggct taccgaacag cagtaacgcg ctgcaacagt ggcatcactt
    30481 gtttgaagct gaagggacaa aacgctcacc gcaagcacag cagcatttac aacaattgct
    30541 gcgtaccgga ctgccgacac gtaaacatga aaactggaaa tatacgccgc tggaagggct
    30601 gaccaatagc cagtttgtca gcattgcggg agagatatcc ccacagcagc gagatgcctt
    30661 agcgttaacg ttagacgctg tgcggctggt atttgtcgat ggacgttacg tgtcagcact
    30721 gagcgatgcg actgaaggca gcgggtatga agtgagcatt aacgacgacc gtcagggggt
    30781 acccgacgct attcaggcgg aagtgtttct gcatttgacg gaaagcctgg cacaaagcgt
    30841 gacgcatatc gccgtgaagc gcggtcaacg gccggcaaag ccattgctgt taatgcatat
    30901 tacccagggc gtggcaggtg aagaggtcaa cactgcccat taccgacatc atctggatct
    30961 ggcggaaggt gccgaagcaa cggtgatcga acattttgtc agccttaatg atgctcgtca
    31021 cttcaccggc gcacggttca ctatcaatgt cgcagcgaac gcccacttgc agcatatcaa
    31081 gctggcgttt gaaaacccgg tcagtcacca ctttgcccat aacgatttgt tgctggctga
    31141 cgatgccacc gcatttagcc atagtttcct gctgggtggc gcagtgttac gacacaacac
    31201 cagtacgcaa ctcaatggcg aaaacagcac gctgcggatc aatagcctgg cgatgccggt
    31261 gaaaaacgag gtgtgtgata cccgtacctg gctggaacac aataaaggtt tttgtaacag
    31321 ccgacagttg cacaaaacta tcgtcagcga caaaggccgc gcggtattta acggtttgat
    31381 caacgtcgcg cagcacgcca tcaaaacgga tggtcagatg accaacaata atctgctgat
    31441 gggcaaactg gcagaagtgg atacgaaacc gcagctggaa atctatgcag atgatgtgaa
    31501 atgcagccac ggcgcgacgg tggggcgtat tgatgatgaa cagatgttct atctgcgctc
    31561 gcgcgggatc aatcagcagg atgcccagca gatgatcatt tacgcctttg ctgccgaact
    31621 gacggaagca ctgcgtgatg aggggcttaa acagcaggtg ctggcccgaa tcggtcaacg
    31681 gctgccagga ggtgcaagat gactttttcc gtcgacaaag tgcgggccga ctttccggtg
    31741 ctttctcgtg aggtaaacgg tttgccgctg gcttatctcg acagcgccgc cagtgcgcag
    31801 aaaccgagcc aggtgattga cgccgaggcc gagttttatc gtcatggcta cgcggcggtg
    31861 catcgcggta ttcatacctt aagcgcccaa gcgaccgaga aaatggagaa cgtgcgcaag
    31921 cgggcatcgc tgtttattaa tgcccgttcg gcggaagagc tggtgttcgt ccgcggcacg
    31981 acggaaggga tcaatctggt cgccaatagc tggggtaaca gcaatgtgcg ggcgggcgat
    32041 aacatcatca tcagtcagat ggagcaccac gctaacattg ttccctggca gatgctttgc
    32101 gcacgcgttg gcgcagagct gcgtgtgatc ccgctcaatc ccgacggtac gttgcaactg
    32161 gagacgctgc ctacgctgtt tgatgagaaa actcgcctgc tggcaattac tcatgtctcc
    32221 aacgtgcttg gcacagaaaa tccactggcg gaaatgatca cgcttgcgca ccagcatggc
    32281 gcaaaagtgc tggtggatgg cgctcaggcg gtgatgcatc atccggtgga tgttcaggcg
    32341 ctggattgcg acttttacgt gttctccggg cataaactgt atggccccac cggaattggc
    32401 attctttatg tgaaagaagc cttgttgcag gagatgccgc cgtgggaagg gggcggttct
    32461 atgatcgcca ccgtcagcct gagtgaaggc acaacctgga ccaaagcacc ttggcggttt
    32521 gaagccggta cacccaatac cgggggcatc attggtcttg gcgcggcgct ggagtatgtt
    32581 tcggcgctgg ggcttaataa catagccgag tatgaacaga atctgatgca ctacgcgcta
    32641 tcacagctgg aatctgtacc ggatctcact ctctatggtc cgcagaacag gcttggcgtt
    32701 attgctttta atcttggtaa acaccacgcc tatgatgttg gcagttttct cgataactac
    32761 ggcattgctg tgcgtaccgg acatcactgc gctatgccat taatggccta ttacaacgtc
    32821 cctgctatgt gtcgggcgtc gctggccatg tataacaccc atgaagaagt ggatcgtctg
    32881 gtgaccggac tgcaacgtat tcaccgtctg ctgggataac agggaggcac tatggcttta
    32941 ttgccggata aagaaaaatt gctgcgtaat tttttacgct gcgccaattg ggaagagaaa
    33001 tatctctaca ttattgagct gggccagcgt ctgccagaat tacgcgacga agacagaagc
    33061 ccacaaaata gcattcaggg ctgccagagt caggtgtgga ttgtcatgcg ccagaatgcg
    33121 cagggaatta ttgaattgca gggcgacagc gatgcggcga ttgtgaaagg gcttattgcg
    33181 gtcgtcttta ttctctacga tcagatgacg ccgcaggata ttgtcaattt cgatgtgcgt
    33241 ccgtggtttg aaaaaatggc gctcacccaa catctcaccc catctcgttc acaaggtctg
    33301 gaagcgatga ttcgcgcaat tcgcgccaaa gccgctgcac ttagctaaac taaagggaca
    33361 gctttcatcc ggttggctca cattgccgga tggtcaatgt ttacactgac ctgcacagga
    33421 gtgtttcggt ttcaggtgaa cataagcgcc atccgacgtt tcagcgtgag tctccggcaa
    33481 atacaggagg tttacaatga aacgcgcgtc tttgcttaca ctcacgctta tcggcgcttt
    33541 tagcgccatc caggctgcct gggcggttga ttatccgcta ccaccaaccg gaagccgact
    33601 ggttgggcaa aatcaaacgt atacggtgca ggaaggggat aaaaaccttc aggccatcgc
    33661 ccgacgtttt gatactgcgg caatgttgat ccttgaagcc aataacacta tcgccccggt
    33721 gccaaaaccc ggtacgacga taactattcc ttcgcaactg ttattacctg atgcgccgcg
    33781 tcaggggatt atcgttaacc ttgcagagct gcgcctttat tattatccgc cgggagaaaa
    33841 tatcgtgcag gtttatccga taggtattgg cttgcagggg ctggaaacgc cggtgatgga
    33901 aacgcgtgtg gggcagaaaa tccctaaccc aacctggacg cctactgcag gcattcgtca
    33961 acgttcgctg gagcgtggta ttaaattacc gccagtcgtt cctgccggac caaataaccc
    34021 gctaggacgt tacgcactgc gcctcgcgca tggtaatggc gaatacctca ttcatggcac
    34081 cagtgcgccg gacagcgtcg gtttgcgcgt cagttcaggg tgtattcgca tgaatgcacc
    34141 ggatattaaa gccttgttct ccagcgtgcg gacgggaacg ccggtgaaag tgatcaacga
    34201 acctgtgaaa tattccgtgg agcctaacgg gatgcgttat gttgaagtac atcgaccact
    34261 atcggcagaa gaacagcaga acgttcagac aatgccatac acactgccag caggctttac
    34321 gcaatttaaa gacaataagg ctgtagatca gaagttagtc gataaagcgt tgtatcgtcg
    34381 ggcagggtat ccggtttcgg tgagcagtgg agcaactccc gcagccagca atgcgccttc
    34441 agtagagtca gcgcagaatg gtgaaccaga gcaagggaat atgttacgcg cgacgcagta
    34501 gcggtaaacg gcagacaaaa aaaatggcgc acaatgtgcg ccatttttca cttcacaggt
    34561 actattactt gcggtattta gtagccatgt tgtccagacg ctggttagca cgagctgcgt
    34621 catctttagc agcctgaacg tcggaacgca ttgcgttcac gtcgttgctc agctggtcaa
    34681 ctttagcgtt cagagtctga acgtcagaag acagctgatc gattttagcg ttgctggagc
    34741 aacctgccag cagagtagaa cccaggatta ccgcgcccag taccagttta gtagctttca
    34801 ttattaatac cctctagatt gagttaatct ccatgtagcg ttacaagtat tacacaaagt
    34861 tttttatgtt gagaatattt ttttgatggg aaggcactta tttttgatcg ttcgctcaaa
    34921 gaagcatcga acgctgggtt ttagataaaa aa
//
LOCUS       DAZGXZ010000043        34598 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000043 DAZGXZ010000000
VERSION     DAZGXZ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.043"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            29..3763
                     /gene="yfaL"
                     /locus_tag="ACHKJF_002159"
     CDS             29..3763
                     /gene="yfaL"
                     /locus_tag="ACHKJF_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP0949515.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAQFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3891..4613)
                     /gene="ubiG"
                     /locus_tag="ACHKJF_002160"
     CDS             complement(3891..4613)
                     /gene="ubiG"
                     /locus_tag="ACHKJF_002160"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP0949516.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4760..7387
                     /gene="gyrA"
                     /locus_tag="ACHKJF_002161"
     CDS             4760..7387
                     /gene="gyrA"
                     /locus_tag="ACHKJF_002161"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP0949517.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7536..9224
                     /gene="yfaA"
                     /locus_tag="ACHKJF_002162"
     CDS             7536..9224
                     /gene="yfaA"
                     /locus_tag="ACHKJF_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP0949518.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9221..9844
                     /gene="yfaT"
                     /locus_tag="ACHKJF_002163"
     CDS             9221..9844
                     /gene="yfaT"
                     /locus_tag="ACHKJF_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP0949519.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9868..14382
                     /locus_tag="ACHKJF_002164"
     CDS             9868..14382
                     /locus_tag="ACHKJF_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP0949520.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLIEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLGDA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14383..16032
                     /gene="yfaQ"
                     /locus_tag="ACHKJF_002165"
     CDS             14383..16032
                     /gene="yfaQ"
                     /locus_tag="ACHKJF_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP0949521.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16037..16813
                     /gene="yfaP"
                     /locus_tag="ACHKJF_002166"
     CDS             16037..16813
                     /gene="yfaP"
                     /locus_tag="ACHKJF_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP0949522.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17088..19937
                     /gene="rcsC"
                     /locus_tag="ACHKJF_002167"
     CDS             17088..19937
                     /gene="rcsC"
                     /locus_tag="ACHKJF_002167"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP0949523.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20137..20787)
                     /gene="rcsB"
                     /locus_tag="ACHKJF_002168"
     CDS             complement(20137..20787)
                     /gene="rcsB"
                     /locus_tag="ACHKJF_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP0949524.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20832..22121)
                     /locus_tag="ACHKJF_002169"
     CDS             complement(20832..22121)
                     /locus_tag="ACHKJF_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0949525.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGCFDYAAQFPNSPNLKHFGLGKKEITIKALSEKWLELKKMEITEN
                     AMVRYKSAIRNCLDCLGGNKLASSITQEDVLIFRKELLTGYQLPGKNQRKPIKGRSVP
                     TVNSYMTIISGMFRFAANNGYIGKNPFSELPSLKRAKTPPDPLTKDEFARLIDACHHQ
                     QTKNFWSLAVYTGMRHGELCGLAWEDIDLEAGTLTVKRNYTQTKEFTLPKTDAGTNRV
                     IQLIQPAIDVLKNQATLTRLGKQYQIEVKLREYGRTSTHSCSFVFNPQILDRTGNSGV
                     HYAVTSINRTWESALKRAKIRHRKAYQSRHTYACWALTAGANPNFIANQMGHANAQMV
                     YNVYGAWMKDNNQSQVEVLNRKLCTYVPQVPQRLNKA"
     gene            complement(22155..22409)
                     /gene="xisR"
                     /locus_tag="ACHKJF_002170"
     CDS             complement(22155..22409)
                     /gene="xisR"
                     /locus_tag="ACHKJF_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0949526.1"
                     /translation="METIIYQITPSKWCTERTLIAATGLKSGTIARARKKSWLLGKEY
                     RHYAAEGEPGPFSECVYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(22417..22563)
                     /locus_tag="ACHKJF_002171"
     CDS             complement(22417..22563)
                     /locus_tag="ACHKJF_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949527.1"
                     /translation="MNREFEIWIRLRYGYRYDLTRDAHGFYCREVVKRMFDVWCHCRG
                     LKIV"
     gene            complement(22584..22826)
                     /locus_tag="ACHKJF_002172"
     CDS             complement(22584..22826)
                     /locus_tag="ACHKJF_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949528.1"
                     /translation="MGEMVDINDELVRTVITVDDGCDHTQRFIWMMNTRRNIRNKVFR
                     HEPKPVRVTKIRIEARKRRKYRPRKAWAPAQPDELA"
     gene            complement(22819..23721)
                     /locus_tag="ACHKJF_002173"
     CDS             complement(22819..23721)
                     /locus_tag="ACHKJF_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949529.1"
                     /translation="MKEADLKALRDAAQNTVYGDKGCDLEAFHSLATPPVILALISHI
                     ENLEKKLDEIVTSEISPKAWLNNLTGEVCSIDAMPDARNDRETYTPLYPAWQRIAAAD
                     DNGRVAWMYTHKKAGYRVPTMTSWESLAEERGGSLDKYIVSPLFTVPQIPPVRTEQDA
                     YPDVQVSWDATRHYVNGWNACRQAMLTTAEKVLDVRIPEYIGRWLSAALHDQNSCEEM
                     KNDIRRFLAEYHHVPSKYNGWRLVPDKLTEQMKYRIVPLLATIKPRSVDEYDRSGENA
                     QFLEWVWTQLMEATKFAYEGSVNG"
     gene            complement(23718..23942)
                     /locus_tag="ACHKJF_002174"
     CDS             complement(23718..23942)
                     /locus_tag="ACHKJF_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949530.1"
                     /translation="MIISFTDYGEQAKLIITSSRLTLRKHNRVVDAVLLCTPDITESR
                     SGFFLVKSSLSGKTRDILRAYKTATRESER"
     gene            complement(23959..24321)
                     /locus_tag="ACHKJF_002175"
     CDS             complement(23959..24321)
                     /locus_tag="ACHKJF_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HFP0949531.1"
                     /translation="MNSTTAAIMNYDPNMTLCGRMARQTVRLTLGQWEYRETFEVDVV
                     GNLTGLDVITCAIENLYENLPYEEAQDEYTGETNIMATIQIGDLACTDEDMLGEAWLA
                     GMLIAAEIISIEPAGTLS"
     gene            complement(24314..25180)
                     /locus_tag="ACHKJF_002176"
     CDS             complement(24314..25180)
                     /locus_tag="ACHKJF_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP0949532.1"
                     /translation="MKKSTELVPVAARDLQIIEYRGQRVVTTEQLAAGYGATPIRIRQ
                     NHSRNEERFVVGKHYFLLEGDELRELKHRVSQSYSVKIASNVRSLILWTERGAANHAK
                     LLETDQAWNYHEDLVEFYFTQRENIDVQRVLTRKELALMVIEAEERAEAMALENKTLS
                     ATVDSLEKHFSKGMTVPQFCRALNGVNTSQIMWWLSGRKWVFNEQRDPEKTPRWRTAS
                     YARDRYLTEEAVQITPHGKATFTKFTPVLLEKGCHRLYQFYMNGELPMKKNWNGEFKH
                     DKAIYTPEAEHE"
     gene            complement(25204..25788)
                     /locus_tag="ACHKJF_002177"
     CDS             complement(25204..25788)
                     /locus_tag="ACHKJF_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949533.1"
                     /translation="MTATEQLFFLTAEGIHTPYGLLDINKTLEMLDEGCFDEPIWHGL
                     EIVCCISEAEEKGMTGELAPEKTIMYWRWLYTAMFFSEQLTRNGWTEVLNENGGTDKG
                     TMYRGHNGGMTIYLKPLRLAMQNYMEWALIETYGAESGLLKSILMYKAFTGALGDGEK
                     TGITMEGYRAMHMILDGLISEATDNSEPETRVFH"
     gene            complement(25775..26335)
                     /locus_tag="ACHKJF_002178"
     CDS             complement(25775..26335)
                     /locus_tag="ACHKJF_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949534.1"
                     /translation="MKEEPEIIEINSEEFTENEWLTETDPRVLALTMEGYIVFPPFFS
                     DAEYSAINNAGIDRAFHKYELDCGPVYRVCRHGQVKKCFTRKTAIIHLAHFMTTKVFE
                     SSGFEKRLPDQRLMHPEYGEVFHPGEITPEYYRAHGRCFHRLLRILARRKQFDAWMEK
                     YNAWIDQYCRLVAQRPFKKRANHDCN"
     gene            complement(26508..26702)
                     /locus_tag="ACHKJF_002179"
     CDS             complement(26508..26702)
                     /locus_tag="ACHKJF_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949535.1"
                     /translation="MNNCVEVLDNVHCEITESLSLLQVIIDSGDIGSPDALNMMNCLF
                     RSLSCTADKAEKHLCRTENE"
     gene            complement(26743..27105)
                     /locus_tag="ACHKJF_002180"
     CDS             complement(26743..27105)
                     /locus_tag="ACHKJF_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949536.1"
                     /translation="MKDEYISAYRRRITVAALRRMKRKTGSNLLTVKRHDGELTTIEI
                     TEQFISQLLLRFEGVTRGEFGRKDGETAIRTAYQDAVGINQHGEYLTESGKLIIDELL
                     NECIDYIKEGHITGCVNH"
     gene            complement(27280..27480)
                     /locus_tag="ACHKJF_002181"
     CDS             complement(27280..27480)
                     /locus_tag="ACHKJF_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949537.1"
                     /translation="MKTPLIMLEEVAAEIKENTSMLEFIFKNSGDNGETDDFLLCLIR
                     SMNKTCEKAYEYVDALRTNKGN"
     gene            complement(27477..27668)
                     /locus_tag="ACHKJF_002182"
     CDS             complement(27477..27668)
                     /locus_tag="ACHKJF_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949538.1"
                     /translation="MKMFKGLTNEPETAFHHIAVLLEAGLIISASGDEECDELSDDIF
                     LLAQQYARSACDAFKEQRT"
     gene            complement(27670..28047)
                     /locus_tag="ACHKJF_002183"
     CDS             complement(27670..28047)
                     /locus_tag="ACHKJF_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0949539.1"
                     /translation="MSTSQLMLIEQVESLLHESLESVSERNRHVLLEVGIEKIQQLKH
                     ILLPPRNKKDCRDGIRTQRESCGLSTAELATLLGVDEETIIQWENGKAEPLASQVIPL
                     ANILDCDPMWLLTSFHSDTTKES"
     gene            complement(28133..28792)
                     /locus_tag="ACHKJF_002184"
     CDS             complement(28133..28792)
                     /locus_tag="ACHKJF_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000349381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0949540.1"
                     /translation="MDHDSFGGRLLMRRVQLGMSQDDLAKLTGVSRVTISKIELGDSQ
                     DTRSSNLFRIADALKCSPKWLLDGSAPSPTDSIQNVRNPKPVSPNRYRYPKLTWVSAG
                     PWNCEDYIAYPEEWIGSDIYAGDHGFWLEVKGDSMTSQNGEFSIFEGMNILVNPDKEV
                     FSGKYVVARLKNSGEATLKQYIEDAGQCFLKPLNPRYPLIPINGDCEIIGVVVEMRMQ
                     I"
     gene            29132..29425
                     /locus_tag="ACHKJF_002185"
     CDS             29132..29425
                     /locus_tag="ACHKJF_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949541.1"
                     /translation="MSPDYVQMEMPARYSQADSEWIKRQLQSLPSSLRQKVALKYAEV
                     YEVTFDSEPVSFRKENRARHEANVRLRRFVETHGRALQGYTTQPPLAGTQQRT"
     gene            29667..30032
                     /locus_tag="ACHKJF_002186"
     CDS             29667..30032
                     /locus_tag="ACHKJF_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949542.1"
                     /translation="MSAELKQVLINLLEEEFGHGMDKVTFDYVLQKKIKSLGCELQRC
                     FTVRLKGDRRGFIDLLVISPDGQRCAVEVDNRSPRQRSLMKIRALPEGISGFVFLRDG
                     KHPQRYIDGVDVIRATRFK"
     gene            30066..31724
                     /locus_tag="ACHKJF_002187"
     CDS             30066..31724
                     /locus_tag="ACHKJF_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFP0949543.1"
                     /translation="MLNIKPNFAQERALNMLRHDWKTYQSFMMYMPTGSGKTGLAAFI
                     TAGLVSRGMRVLFVVPYTILINQTAQRFVQYGLSGDEISFIWRDHPNNDPSRLIQIAS
                     ADTLIRRDFPQNIDLLIIDEAHLRKRRILTEIERLVAEGNVKVIGLSGTPFSSFLGRY
                     YQRLLKPTTIGELIQRGELSNYEFYAPTKPDLEGVKTKVSMEYGRDYDESQLAEIMCG
                     ADLVGDIVDNWLCNGRDLPTVAFCVNKTHASFVTMQFNKAGINAEVMVAETPHDERQL
                     MISRFETGATKIIVSVGVLVAGFDSDVRCIIYARPTKSEIRWLQAIGRGLRTAPGKES
                     CLIFDHSGTVHRLGFPENIEYDELPGKNDGMQVSSSRGNEIREEKLPKECPGCHFMKP
                     AGVYVCPKCGFKPLAGEDVNTDTRRNIRKLSKDEKIYTKSDKQAWWSQIKFYQRQRAS
                     LGRPVSDGWCAHTFREKFGEWPDGLSSFPMEITPEVSNYIRHKFIRFARGRSRAEKMT
                     EKSPEALSLSLLHGVRSGVPEGSESWQIMQAKQQLQKNRNSLSQ"
     gene            31726..32694
                     /locus_tag="ACHKJF_002188"
     CDS             31726..32694
                     /locus_tag="ACHKJF_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HFP0949544.1"
                     /translation="MKTAEAAKGHWPEILKHYGLPPVTGKKHFKGECPVCGARGKFRI
                     DDRDGTGTWICVCGSGDGMKLIALTQKRAFHEICAEIDRITGNEYRRDKPPVICTSES
                     LRSRVQRRFSALTALQGTSGAEYLRSRGIFSLPVEGVRFNSQQNYNGRMFQSLYALAT
                     DDKGELCYLHQTLLDGAKKADIGISARRLKSLQDDNYLDHARSVAIRMFPVASTLGIA
                     EGIETALSAHQIYKVNTWATINSGFMKKFRVPAGVTHLIIFADRDEYSATGMAAACEC
                     AHANLQARNDIQRVSVRWPDHDDFNNMLMNGDQVRELVFYRKKAVA"
     gene            32694..33557
                     /locus_tag="ACHKJF_002189"
     CDS             32694..33557
                     /locus_tag="ACHKJF_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0949545.1"
                     /translation="MRTDNQVHKALFTIPTAAYSAVPANIKPLPEQRRITGHKQTDAY
                     LWILEVIHLNEAVHLDAAEAALEKLKITPEEASERYGRYLQEINVDPFQIAFATIGMD
                     NPAQAIRNARENIKKAASVRATFGSYEAALDDVEAERIIRTSPKFIDDYYWGWTAAEK
                     KAGSIDGVRSNEIDDQRRAYVDGYCDVLPEPHTLSDVVREFIYWDWLYEMRQTAGRET
                     GDKYGFTGEHHESVYDRQFWLENLLGKIKPVTRDEAVEVCRWFLASGKDEYMEDNGSA
                     VILNLVGECEQ"
     gene            33636..34061
                     /locus_tag="ACHKJF_002190"
     CDS             33636..34061
                     /locus_tag="ACHKJF_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0949546.1"
                     /translation="MRDIQQVLSRWGAWVVNNHESVAWSGVAAGFKGVIPSKVKSRPQ
                     CTDDDALIISNCMAQLNVNNSDLHDFLYDYYVFGMTLMGLGRKHERSDCWAGRVLQKA
                     EGVIEGMLIMQGIKLEMDRYVERERSGAQVSQFSGHTGN"
     gene            34261..34416
                     /locus_tag="ACHKJF_002191"
     CDS             34261..34416
                     /locus_tag="ACHKJF_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0949547.1"
                     /translation="MKQQKAMLIALIVICLTVILTVLVARKDLCEVRIRTGQTEVAVF
                     VDYESEK"
BASE COUNT         8419 a         8220 c         9162 g         8797 t
ORIGIN      
        1 tcttatataa aatgatggtc agattaatat gcggattatc tttctacgca aggagtattt
       61 atctctactc ccgtcaatga ttgcatctct tttctctgct aacggtgtcg cggcggccat
      121 tgatttatgc cagggatatg atatcaaagc gagttgtcac gccagcaggc aaagcctttc
      181 aggcattacg caggactgga gtgtcgccga tgggcaatgg ctggtttttt cggatatgac
      241 caataatgcc agcggaggag ccgtattttt gcaacaagga gcgcaatttt cactattacc
      301 agaaaatgaa actggaatga ctctgtttgc caataacacc gttacaggag aatataataa
      361 cggcggggcc atatttgcta aagaaaactc aacgctgaat cttactgatg ttattttttc
      421 cggtaacgtc gcaggcggct atggtggcgc aatctattct tctggtacta acgatactgg
      481 tgccgtcgat ttacgtgtca ctaacgccat gtttcgcaat aacatcgcta atgatggcaa
      541 aggtggcgca atttatacca ttaataatga cgtttattta agtgatgtta tttttgataa
      601 caaccaggca tatacatcaa caagttacag tgatggcgat ggcggggcaa tcgatgttac
      661 cgataataat agcgacagca agcatccttc aggttatacg ataataaata acaccgcctt
      721 tacaaataac actgccgaag gttatggcgg ggcgatatat accaatagcg cgacggctcc
      781 ctatcttatt gatatttctg ttgatgacag ctacagccag aacggcggcg tgttagtgga
      841 tgagaacaac agcgcagcag gctatggaga tggtccttcc actgcggcgg gtggctttat
      901 gtatctcggc ttaagtgaag ttacctttga tattgccgac ggaaaaacgc tggttattgg
      961 caatacagag aatgacggag ctgttgactc tattgctggt accgggttga tcaccaaaac
     1021 aggttccggc gatctggtac ttaatgcaga taacaatgac tttactggtg agatgcagat
     1081 tgaaaacggt gaagttaccc tgggtcgcag caactcactg atgaatgtcg gcgatacgca
     1141 ttgccaggac gatccgcaag actgctacgg tctgacgata gggagtattg ataagtacca
     1201 gaatcaggca gagctaaacg ttggctccac ccaacaaacc tttgtgcact cattgacggg
     1261 ctttcagaat ggcactttaa atatcgatgc tggtggcaat gttactgtta atcaaggtag
     1321 ttttgctggc accatcgaag gtgctggtca gctcaccatt gcgcaaaacg gcagctacgt
     1381 gctgtcgggg gcgcagtcga tggcgctaac cggcgatata gtcgttgatg atggtgcggt
     1441 gctttcgctg gaaggcgacg cggcagatct taccgctctc caggacgatc cgcagtcgat
     1501 cgtgttaaac ggcggtgtgc tcgatctctc tgatttctcc acctggcaga gtggcacatc
     1561 atacaacgat ggccttgaag tcagcggcag cagcggaacg gttatcggca gtcaggatgt
     1621 ggtagatctt gcaggtggaa acgatatgca tatcggcggt gacgggaaag atggcgtcta
     1681 cgtggtggtc gatgcgggcg acgggcaggt aagtctggca aacaataata gttatttggg
     1741 cacaacacaa atcgcctccg gtatgctgga ggtgagcgac aactcacagc ttggagatac
     1801 ccactataac cgccaggtta tctttaccga taagcaacaa gaaagcgtga tggagattac
     1861 tgccaatgtc gatactcgct ctacgacgac tgagcatggg cgtgatattg aaatgcgcgc
     1921 cgacggtgaa gtggcagttg atgcgggggt agacacgcag tggggcgcac tgatggctga
     1981 cagcagcggg cagcatcagg atgagggtag cacattgact aaaacggggg cgggtacgct
     2041 ggagctgacc gccagcggta caacgcagtc ggcggtacgt gtcgaagaag gcaccctgaa
     2101 aggcgatgtc gcggatatcc ttccttatgc ttcgtcactg tgggtcggtg atggggcgac
     2161 gttcgttact ggcgcggatc aggatattca atctattgat gctacttcca gcggcactat
     2221 cgacatcagc gatggtacgg ttttgcgcct gaccgggcag gatacttctg tcgcccttaa
     2281 tgcctcactg tttaacggcg atgggacgct ggtgaatgcc accgatggcg tgacgttgac
     2341 aggtgagctt aataccaacc ttgaaactga cagcctgacc tatcttgccg acgtgacggt
     2401 taatggcaat ctgaccaata cgtccggtgc ggttagcctg caaaatggcg tcgctggcga
     2461 tacgctgacg gtaaacggtg attataccgg cggcggtacg ctactgctcg atagcgaatt
     2521 aaacggcgat gactcggtaa gcgatcaatt ggtgttgaac ggtaatactg ctggcaacac
     2581 aactgtggtg gttaactcca ttacagggat tggtgagccg acatcgacag gcattaaagt
     2641 ggttgatttc gcagccgatc ccacgcagtt tcaaaacaat gcgaagttca gtctggcagg
     2701 cagcggctac gtcaatatgg gagcatatga ctacacgctg gtggaagata acaacgactg
     2761 gtatctgcga tcgcaagaag taacgccacc attgccacct gatccagacc ctgacccaac
     2821 acccgatcct gacccaacac ccgatccaat ccccgcttac cagccggtgc tgaatgccaa
     2881 agttggcggt tatctcaata atctgcgggc ggcaaatcag gcgtttatga tggagcgacg
     2941 cgatcatgca ggtggcaatg gtcagacgct gaatttacgt gttatcggcg gacgttatca
     3001 ttacacggcg gcagggcaac tggctcagca tgaagacact tctacggtgc agcttagcgg
     3061 tgacctgttt agcgggcgct ggggcgatga tggcgagtgg atgcttgggg cggttggtgg
     3121 ctacagtgat aatcagggcg agagccgctc gaatatgacc ggaactcgcg ccgataacca
     3181 gaaccacggt tatgccgttg ggctgacctc aagctggtat cagcacggta atcagaagca
     3241 gggggcctgg ctggatagct ggctgcaata cgcgtggttt aacaatgatg tttccgaaca
     3301 agacgatggc acagatcatt accactcgtc ggggattatc gcctcgctgg aagccgggta
     3361 tcagtggtta ccggggcgtg gtgtggtgat tgaaccgcag gcgcaggtga tttatcaggg
     3421 cgtgcagcag gatgatttta ccgccgctaa ccatgcgcgc gtgtcacaat cgcaggggga
     3481 tgatattcag acgcggctgg gtttacacag cgaatggcgt accgcggttg gtgtcacacc
     3541 aacattagat ctgaattatt atcacgatcc ccatgcgacg gaaattgaag aagatggcag
     3601 cactatcagt gacgatgcgg tgaagcgacg gggtgaaata aaagtgggag tcacgggcaa
     3661 tatcagtcag cgagtgtccc tgcgcggcag cgtggcgtgg cagaaaggga gtgatgattt
     3721 tgcccagacg gcagggtttt tgtcgatgac ggtgaaatgg taatcactgt ctagtcggca
     3781 cggttgccgg atgcggcgta aacgccttat ccggcctacg gggagcattt gtaggcctga
     3841 taagacgcgg caagcgtcgc atcaggcatt ggctcggcca aacaacaacc tcacttattc
     3901 tgcgtgtgca gcatatagtt cacatccacg ccggggccga gtttaaaagt attagtgagc
     3961 gggttgtaat gtagcccggt tatatgtcgc tctttaaggc ttgtctgatc tacccagcca
     4021 agcagttctg ccggtttaat aaacttcttc acatcatgcg tgcctttggg caccatgcgc
     4081 aaaatatatt ccgcgccaac caccgccatc agccatgact tgccgttgcg gttaagcgtc
     4141 gagaaaaaga catcgccgcc tggtttcacc agttgcgcac aggctctgac cactgactgc
     4201 ggatcgggga cgtgctccag catctccatg caggtcacca catcatactg cccggcatgt
     4261 tttgccgcgt gctcttccac ggtttcctgc acataatcca cctgaatgcc gctttccagc
     4321 gcgtgcagtt ttgccacctg caatggctca aagcccatat ccagaccggt caccgtcgcg
     4381 ccttcgcgcg ccatactctc ggccagaatg ccgccgccac aaccgacatc gagcaccttt
     4441 ttgccaaata aaccgccagc acgctcggca atatagccca gacgcagcgg gttaatgcgg
     4501 tgcaacggtt tgaactcacc ttcaagatcc caccagcggg aggcgacggc ttcaaattta
     4561 gcgatctctt cgtggtctac gttatggttt tccggcgatt tttcggcatt cattggcact
     4621 tctactccgt aattggcaag acaaacgagt atatcaggca ttggatgtga ataaagcgta
     4681 taggtttacc tcaaactgcg cggctgtgtt ataatttgcg acctttgaat ccgggataca
     4741 gtagagggat agcggttaga tgagcgacct tgcgagagaa attacaccgg tcaacattga
     4801 ggaagagctg aagagctcct atctggatta tgcgatgtcg gtcattgttg gccgtgcgct
     4861 gccagatgtc cgagatggcc tgaagccggt acaccgtcgc gtactttacg ccatgaacgt
     4921 actaggcaat gactggaaca aagcctataa aaaatctgcc cgtgtcgttg gtgacgtaat
     4981 cggtaaatac catccccatg gtgactcggc ggtttatgac acgatcgtcc gtatggcgca
     5041 gccattctcg ctgcgttaca tgctggtaga cggtcagggt aacttcggtt ccatcgacgg
     5101 cgactctgcg gcggcaatgc gttatacgga aatccgtctg gcgaaaattg cccatgaact
     5161 gatggccgat ctcgaaaaag agacggtcga tttcgttgat aactatgacg gcacggaaaa
     5221 aattcccgac gtcatgccaa ccaaaattcc taacctgctg gtgaacggtt cttccggtat
     5281 cgccgtaggt atggcaacca acatcccgcc gcacaacctg acggaagtca tcaacggttg
     5341 tctggcgtat atcgatgatg aagacatcag cattgaaggg ctgatggaac acatcccggg
     5401 gccggacttc ccgacggcgg caatcattaa cggtcgtcgc ggtattgaag aagcttaccg
     5461 taccggtcgc ggcaaggtgt atatccgcgc tcgcgcagaa gtggaagttg acgccaaaac
     5521 cggtcgtgaa accattatcg tccacgaaat tccgtatcag gtaaacaaag cgcgcctgat
     5581 cgagaagatt gcggaactgg taaaagaaaa acgcgtggaa ggcatcagcg cgctgcgtga
     5641 cgagtctgac aaagacggta tgcgcatcgt gattgaagtg aaacgcgatg cggtcggtga
     5701 agttgtgctc aacaacctct actcccagac ccagttgcag gtttctttcg gtatcaacat
     5761 ggtggcattg caccatggtc agccgaagat catgaacctg aaagacatca tcgcggcgtt
     5821 tgttcgtcac cgccgtgaag tggtgacccg tcgtactatt ttcgaactgc gtaaagctcg
     5881 cgatcgtgct catatccttg aagcattagc cgtggcgctg gcgaacatcg acccgatcat
     5941 cgaactgatc cgtcatgcgc cgacgcctgc agaagcgaaa actgcgttgg ttgctaatcc
     6001 gtggcagctg ggcaacgttg ccgcgatgct cgaacgtgct ggcgacgatg ctgcgcgtcc
     6061 ggaatggctg gagccagagt tcggcgtgcg tgatggtctg tactacctga ccgaacagca
     6121 agctcaggcg attctggatc tgcgtttgca gaaactgacc ggccttgagc acgaaaaact
     6181 gctcgacgaa tacaaagagc tgctggatca gatcgcggaa ctgttgcgta ttcttggtag
     6241 cgccgatcgt ttgatggaag tgatccgtga agagctggag ctggttcgtg aacagttcgg
     6301 tgacaaacgt cgtactgaaa tcaccgccaa cagcgcagac atcaacctgg aagatctgat
     6361 cactcaggaa gatgtggtag tgacgctctc tcaccagggc tacgttaagt atcagccgct
     6421 ttctgaatac gaagcgcagc gtcgtggcgg gaaaggtaaa tctgccgcac gtattaaaga
     6481 agaagacttt atcgaccgac tgctggtggc gaacactcac gaccatattc tgtgcttctc
     6541 cagccgtggt cgcgtctatt cgatgaaagt ttatcagttg ccggaagcca ctcgtggcgc
     6601 gcgcggtcgt ccgatcgtca acctgctgcc gctggagcag gacgaacgta tcactgcgat
     6661 cctgccagtg accgagtttg aagaaggcgt gaaagtcttc atggcgaccg ctaacggtac
     6721 cgtgaagaaa actgtcctca ccgagttcaa ccgtctgcgt accgccggta aagtggcgat
     6781 caaactggtt gacggcgatg agctgatcgg cgttgacctg accagcggcg aagacgaagt
     6841 aatgctgttc tccgctgaag gtaaagtggt gcgctttaaa gagtcttctg tccgtgcgat
     6901 gggctgcaac accaccggtg ttcgcggtat tcgcttaggt gaaggcgata aagtcgtctc
     6961 tctgatcgtg cctcgtggcg atggcgcaat cctcaccgca acgcaaaacg gttacggtaa
     7021 acgtaccgca gtggcggaat acccaaccaa gtcgcgtgcg acgaaagggg ttatctccat
     7081 taaggttact gaacgtaatg gtttagtggt tggcgcggta caggtagatg actgcgacca
     7141 gatcatgatg atcaccgatg ccggtacgct ggtacgtact cgcgtttcgg aaatcagcat
     7201 cgtaggccgt aacacccagg gcgtgatcct catccgtact gcggaagatg aaaacgtagt
     7261 gggtctgcaa cgtgttgctg aaccggttga cgaggaagat ctggatacca tcgatggcag
     7321 tgccgcggaa ggggacgatg aaatcgctcc ggaagtggac gttgacgacg agccagaaga
     7381 agaataattt tacttcttca tgccaaaagg gagctatctc ccttgtttga attgaaaagt
     7441 ccaggctgca aagtctgggc ttttgtcgta ttagggcacg gtaaagtttg gctgtgcccg
     7501 taaaaaatgg ctggctatac acaaggaatg tggcaatgag tggtgaaaaa aaggcgaaag
     7561 gctggcggtt ctatggtctt gtaggttttg gcgcaatagc actgctttcc gctggcgtct
     7621 gggcgttgca atatgctggc agtgggccag aaaaaacgtt gtcgccgctg gtggtgcaca
     7681 acaatctgca aatcgatctc aatgagccgg acctctttct cgacagcgac tctctgagcc
     7741 agcttcccaa agatctcctc accattccgt ttctccacga tgttctgagc gaagatttcg
     7801 ttttctatta tcagaatcat gccgatcgtc tgggcattga aggcagcatt cgtcgtattg
     7861 tctatgaaca cgatctcacg ctgaaagata agctcttttc gtcactctta gatcagcccg
     7921 cgcaggcagc gctgtggcac gataaacaag gccatctttc acattatatg gtgctgatcc
     7981 agcgcagtgg tttaagcaaa ctgctggagc cattgttgtt tgccgctacc agcgacagcc
     8041 agttaagcaa aacggaaatc agtagcatca agataaatag tgaaactgtt cctgtttatc
     8101 agttgcgcta taacggcaat aacgccctga tgttcgcgac ttatcaggac aagatgctgg
     8161 tgttttccag cacggatatg ttgtttaaag atgatcagca ggataccgaa gccacggcga
     8221 tcgcaggtga tttgttgagc ggcaaaaaac gctggcaagc aagctttggc ctggaagagc
     8281 gtgctgctga aaaaacgcca gtacgccagc gcatcgtggt cagcgccagg ttgctgggtt
     8341 ttggctacca gcggttaatg ccttcttttg ctggcgtacg cttcgaaatg ggtaacgacg
     8401 gctggcacag ttttgtggcg ttaaatgatg aatccgccag cgtagatacc agtttcgatt
     8461 ttacgccggt ctggaacagt atgcctgccg gggccagctt ctgtgtggtg gtgccgtatt
     8521 cacacggtat tgccgaagag atgctttcgc acatcagtca ggaaaacgac aagttgaatg
     8581 gggcgttaga cggtgccgcg gggctgtgct ggtatgcaga ctcaaaattg caaaccccgc
     8641 tgtttgtcgg tcagtttgat ggcactgccg aacaggcgca attgccaggg aaactgttta
     8701 cgcaaaatat tggtgcgcac gaaagcaaag cgccagaagg tgttttgccg ataagccaga
     8761 ctcagcaggg cgaagcgcaa atctggcgtc gcgaagtgag ttcccgatac ggtcagtatc
     8821 cgaaagcgca ggcggcgcaa cccgatcaat taatgtcgga ttattttttc cgcgtgtcgc
     8881 tggcgatgca aaacaaaacg ctgcttttct ccctcgatga cacgctggtt aataacgccc
     8941 tgcaaacact gaataaaaac cgcccggcaa tggtggatgt aatacccact gatggcatcg
     9001 ttccgctcta tatcaatcca caaggcgtgg cgaaactgct gcgtaacgaa acgttgacca
     9061 gtctgccgaa gaatctcgaa ccggtttttt ataacgccgc acaaacttta ttaatgccga
     9121 agctggacgc tttatctcaa caaccgcgtt atgtcatgaa gctggcccag atggaacccg
     9181 gtgccgcctg gcagtggcta cccataacct ggcagccgct atgaggcacg ggctgctggc
     9241 gctgatttgc tggctgtgtt gtgttgttgc ccatagcgaa atgctgaatg tcgaacaatc
     9301 cgggctgttt cgcgcctggt ttgtacgcat tgcacaagaa cagctccgcc aggggccaag
     9361 tccacgctgg tatcagcagg attgtgcggg cctggtgcga tttgcggcga acgagacgct
     9421 gaaagttcac gatagtaaat ggctaaaaag taacggtttc tctagccagt atttgccgcc
     9481 agagatgacg ctaacacctg gacagcgtca actggcgcaa aactggaatc aggggaacgg
     9541 gaaaaccggc ccctacgtga ccgcgattaa tttgattcag tacaacagcc agtttattgg
     9601 ccaggacata aaccaggcgc tgcctggcga tatgattttt ttcgatcagg gcgatgccca
     9661 gcacttaatg gtctggatgg ggcgttacgt catctaccac accggaagcg ccacgaaaac
     9721 tgacaacgga atgcgcgcag tcagtctgca acaacttatg acatggaagg acacccgatg
     9781 gatacccaac gattccaatc ccaatttcat tggcatttat cgtttaaatt ttctggcgcg
     9841 atagcagcgt gcttgtccct gtctcttgtg ggaacagggc ttgctaatgc tgatgattcg
     9901 cttccttcca gtaactatgc gccgcccgcc gggggaacat tctttttgct tgctgacagc
     9961 agttttagca gcagtgaaga ggcgaaagtg cgactggaag cgccggggcg tgattatcgg
    10021 cgctatcaga tggaagagta cggcggcgtg gacgttcgcc tgtatcgtat tcctgacccg
    10081 atggcatttt tgcgccagca gaaaaacctg catcgcattg tggtgcaacc gcaatatctg
    10141 ggcgacgggc tgaacaatac gctgacctgg ctgtgggata actggtacgg caaatctcgc
    10201 cgcgtaatgc agcgtacttt ctcttctcag tcacggcaga atgtgactca ggcattaccc
    10261 gaattacatc tcggcaatgc cattattaaa ccttcccgtt atgtacagaa caaccagttt
    10321 tccccgctga aaaaatatcc cttggtggaa cagttccgtt atccactatg gcaggctaaa
    10381 ccggtcgagc cgcagcaagg ggtaaaactg gaaggcgcat ccagcaattt catctcgccg
    10441 cagccgggta acatttatat tcctctcggc caacaagagc cgggactgta cctcatcgag
    10501 gcgatggttg gtgggtatcg ggcgacgacg gtggtgtttg tttccgatac cgtggcgctt
    10561 agcaaagtgt caggcaacga gcttctggtg tggaccgcgg gtaaaaaaca gggtgaagcg
    10621 aagcccggct cagagatctt gtggactgac ggtcttggcg tgatgacccg cggtgtgacc
    10681 gatgacagcg gtaccttgca gttacaacat atatcgccag aacgttcata cattctgggt
    10741 aaggatgctg aaggcggtgt ttttgtctcc gagaacttct tctacgaaag cgaaatctac
    10801 aacacccgct tgtatatttt taccgatcgc ccgctatatc gcgcaggcga tcgtgtcgat
    10861 gttaaagtga tgggccgcga gttccacgat ccgttgcatt catcccccat cgtcagcgcc
    10921 ccggcgaagc tttcggtgct ggacgctaac ggcagtctgt tgcaaaccgt cgatgtcacg
    10981 ctggatgcgc gcaatggcgg gcagggaagt ttccgcctgc cagaaaatgc cgttgccgga
    11041 ggttatgagt tacgtcttgc ttaccgcaat caggtctata gcagcagttt tcgcgtggca
    11101 aactacatca agccacattt cgagattggt ttagctctcg acaaaaaaga gttcaaaact
    11161 ggcgaagcgg tcagcggcaa actgcaactg ctctacccgg atggcgagcc ggtaaaaaat
    11221 gcccgcgtgc agttaagttt gcgcgctcag caattatcaa tggtcggtaa cgatttgcgt
    11281 tatgccggac gtttccccgt gtcgctggaa ggcagcgaaa cggtgtccga cgccagcggt
    11341 catgtggcgt taaatctccc cgccgccgat aaaccgagcc gttatttgtt aaccgtctcc
    11401 gccagtgacg gcgcggcgta tcgtgtcacc accaccaaag agatcctcat tgaacgcggt
    11461 ctggcgcatt actcattaag tactgccgca caatacagta atagcggcga gtcggttgtg
    11521 ttccgttatg ccgcgctgga atcttcaaaa caggttcctg ttacgtatga atggttgcgt
    11581 ctcgaagacc gcacgagcca tagcggagag ctaccgtcag gcggcaaatc ctttaccgtc
    11641 aatttcgcta aacctggcaa ctacaatctg acattacgcg ataaagacgg cttaattctc
    11701 gctgggttaa gtcatgccgt cagcggtaag ggcagcacgg cgcatactgg tacggtagat
    11761 atcgtggcgg ataaaacgct gtaccagcca ggcgaaactg cgaagatgct gattaccttt
    11821 ccggagccaa ttgatgaagc attattgacg ctggaacgcg atcgcgtgga acagcagtcg
    11881 ctgctttcgc atccggcaaa ctggctgacg ttacaacgtt taaacgatac ccagtatgaa
    11941 gcccgggttc cagtgagcaa ttcctttgcg cctaacatca ctttttcggt gctgtatacc
    12001 cgtaatggtc agtacagttt tcagaacgcc gggatcaaag ttgccgttcc tcagcttgat
    12061 atccgggtga aaacggacaa aacccattac cagcctggtg aactggtcaa tgtcgaatta
    12121 acctcgtcgc tgaaaggtaa acctgtttct gcgcagctaa cggtaggcgt ggtcgatgaa
    12181 atgatctacg cgctgcaacc agaaatcgcg ccgaatatcg gcaaattttt ctatccgctg
    12241 gggcgtaaca atgtgcgtac cagctccagt ctgtcgttta tcagctacga ccaggcgctc
    12301 tccagcgagc cggttgcgcc tggcgcaact aaccgcagcg agcggcgagt aaaaatgctt
    12361 gaacgtccac ggcgtgaaga ggtggatacc gcggcatgga tgccgtcact cacaaccgat
    12421 aaacaaggca aagcgtattt cacgttcctg atgcctgatt cgttaacccg ctggcgtatc
    12481 accgcgcgtg ggatgaacgg cgacgggctg gtcgggcagg ggcgtgctta tctgcgttcg
    12541 gaaaaaaatc tctacatgaa gtggagtatg ccaacggtgt atcgcgtggg cgacaaaccg
    12601 gcggcaggac tgtttatctt cagtcagcag gataacgaac cggtggcgct ggtgactaaa
    12661 tttgcaggcg ctgagatgcg ccagacgctg acgctgcaca aaggggcgaa ttatatttcg
    12721 ctggcgcaga acattcagca atctggcttg ttaagcgcag aactgcaaca aaatgggcaa
    12781 gtgcaggaca gcattagcac aaaactgtct tttgtggata acagctggcc cgttgaacag
    12841 cagaaaaatg tcatgctcgg cggtggcgag aacgcgctga tgttgcccga gcaggctagc
    12901 aatatccggc tacaaagtag cgaaacgccg caggagattt tccgcaacaa tcttgatgcg
    12961 ttagtcgatg gaccatgggg tggcgtgatc aacaccggta gccgtctgat cccgctcagt
    13021 ctcgcctggc gttcgcttgc cgatcatcaa agtgccgccg ctaacgacat tcgtcagatg
    13081 attcaggata accgtctgcg gctgatgcaa ctggcggggc ccggagcgcg ctttacctgg
    13141 tggggtgaag atggcaatgg tgacgccttc cttacggcat gggcatggta cgccgactgg
    13201 caggccagcc aggcgctcgg cgtaacgcaa caaccggaat actggcagca tatgctcgac
    13261 agttatgccg agcaggcgga taacatgccg ttattgcatc gggcgctggt gctggcgtgg
    13321 gcacaggaga tgaatctgcc gtgcaaaacg ttgttgaaag ggttggatga agctatcgcc
    13381 cggcgcggaa ctaaagatga agatttctct gaggaagaca tccgcgatat caatgacagc
    13441 ctgatcctcg atacaccgga atctccactg ggagatgcgg tggcaaacgt cttaaccatg
    13501 acgttgctga aaaaagcgca gttgaagtcc acggtgatgc cacaggttca gcaatatgcg
    13561 tgggataaag cggcaaacag caatcagccg ctggcgcaca cggttgtgct gctcaatagc
    13621 ggtggtgacg ctacccagac ggccgctatt ttaagtggtt tgaccgctga gcaatccact
    13681 attgagcgcg cgctggccat gaactggctg gcgaaatata tggcgacaat gcctccagtt
    13741 gttttgcctg cgcctgcggg cgcatgggct aaacataagt taactggagg gggcgaatac
    13801 tggcgttggg ttggtcaggg tgtgccggac attctctctt ttggtgacga attatcgccg
    13861 caaaatgtgc aggtccgctg gcgtgaaccg gcaaaaacgg ctcaacaaag taacattccg
    13921 gtgaccgttg aacgccagtt gtatcggctt atccccggtg aagaagagat gagctttact
    13981 ctgcagccgg tgaccagcaa tgagattgac agcgatgcgc tgtatctcga tgaaattacg
    14041 cttaccagcg agcaggatgc agttctgcgc tatggtcagg tggaagtacc gctcccgccg
    14101 ggagccgacg ttgagcgcac aacatggggc atttcagtca ataaacccaa cgccgcgaaa
    14161 cagcaggggc aattgctgga aaaagcgcgt aatgaaatgg gcgaactggc ctatatggtg
    14221 ccggtgaaag aactgacggg aacggtcact ttccgccatt tgctgcgctt ctcgcaaaaa
    14281 gggcaattcg ttctgcctcc tgctcgttat gtgcgttcct atgcacctgc gcagcaaagt
    14341 gttgcggcag ggagtgaatg gaccgggatg caggtgaaat aagtgaactg gcgcagaatc
    14401 gtctggctat tggcgttggt tactctgcca acgcttgcgg aggagacgcc gttacaactg
    14461 gtgctgcgcg gtgcgcagca cgatcaactg tatcagctgt cgtcatcagg cgtgaccaaa
    14521 gtctcagcat tacctgactc gctcaccacg ccgttaggga gtttgtggaa actatatgtt
    14581 tacgcctggc tggaggatac ccatcaaccg gagcagccct accagtgtcg cggaaactcc
    14641 ccggaagagg tttattgttg tcaggctggg gagagcataa cgcgcgatac cgcgctggta
    14701 cgttcctgtg ggctctattt tgcgccgcag cggttgcata ttggcgcaga tgtgtggggg
    14761 caatactggc aacagcgtca ggcacctgcg tggttagcgt ctcttacaac gctgaagcca
    14821 gaaacctcgg taacggttaa atcgctgctc gattcattag cgacgttacc tgcgcaaaac
    14881 aaagctcagg aagtcttact ggatgtggtg ctggacgagg cgaaaatcgg tgtcgcatcc
    14941 atgctcggta gtcgggtacg ggtgaaaacc tggagctggt ttgccgatga taaacaggaa
    15001 atccgccagg gtggttttgc tggctggtta accgatggta caccgctgtg ggttactggc
    15061 agtggcacca gcaaaacggt actaacccgt tatgcgacgg tgcttaatcg ggttttgcca
    15121 gtgccgacgc aggttgccag tgggcaatgt gtagaggtgg agctgtttgc ccgctatccg
    15181 ctgaagaaaa tcacagcgga aaagagtacg acggcagtaa aacccggcgt acttaatggg
    15241 cgctatcgtg tgacgtttac taatggtaac cacattacgt ttgtcagcca cggtgagacg
    15301 accttactga gcgaaaaggg caaactgaaa ttgcagtcgc atctcgatcg tgaagagtac
    15361 gttgccagag ttctggatcg cgaggcgaaa agtacgcccc ctgaggcagc gaaagccatg
    15421 accgtggcga ttcgcacttt tttacagcaa aacgccaatc gggaaggcga ttgcctgacc
    15481 atccccgaca gcagcgccac gcagcgtgtt tctgcttcgc cagcgaccac gggggcgcga
    15541 acgatgaccg cctggacgca ggatctcatc tatgcaggcg atccggttca ttatcatggc
    15601 agtcgagcta ccgaagggac actttcctgg cggcaagcca cggcccaggc ggggcaggga
    15661 gagcgttacg accagatact cgcgtttgct tatcccgaca acagccttag ccgctgggga
    15721 gcgccacgct caacctgcca gttattaccc aaagcgaaag cctggctggc gaaaaaaatg
    15781 ccgcagtggc ggcgtatatt acaaggcgag acggggtaca acgaaccaga cgtgtttgcg
    15841 gtctgtcgtt tagtctcagg tttcccctat accgatcggc agcagaaacg gctgtttatt
    15901 cgtaacttct ttacgcttca ggatcggctg gatttaaccc atgagtatct gcacctggcc
    15961 tttgatggtt atcccaccgg gcttgatgag aactatatcg aaacgctgac ccgacaatta
    16021 ttaatggact aatgctatgc gaaagatttt tcttccgttg ttactggtgg cactttcgcc
    16081 tgtcgctcac agtgaaggtg tgcaggaagt cgagattgac gcaccgctct ctggctggca
    16141 tccggcagag ggcgaagatg ccagtttttc acaatcgatc aattacccgg cgtcgtccgt
    16201 caacatggca gatgatcaaa atatctcagc gcagatccgc ggcaaaataa agaattatgc
    16261 tgcggcgggt aaagttcagc agggccggct ggtggtcaac ggtgccagta tgccgcagag
    16321 aattgaatcc gatggttcat ttgcacgccc ttatattttc actgaaggca gcaacagcgt
    16381 gcaggtcatc agcccggatg ggcaaagccg gcaaaaaatg cagttttact caacgccggg
    16441 tacaggaacg attcgtgcac gtttacggct ggttctctcg tgggatacgg acaataccga
    16501 tctcgatctc catgttgtca cgcccgatgg cgaacacgcc tggtacggta acaccgtgct
    16561 gaaaaacagt ggtgcactgg atatggatgt cacgacgggg tacggacccg agattttcgc
    16621 catgccagca ccagttcacg ggcgctatca ggtgtatatc aactactatg gtgggcgcag
    16681 cgaaacggaa ttgacgaccg cccaactgac gctgatcacc gatgaaggat cggtcaatga
    16741 gaaacaggaa acatttattg taccgatgcg taatgctggc gagctgacgc tggtgaaaag
    16801 ttttgactgg tgatccatcc cttcacacaa cctggctctc tcttttttca gataagactg
    16861 ctgggcgatc tgccgacgcc aggatataac cattaacaag cgtcggcaga ttggtcattt
    16921 cttatttttc gtactgagaa ctgtccattt actccggctt tcctggttaa ggcaatggtt
    16981 tctaagcgat acgcattatt gcaatggtga cagaacattg cggtaacacg cttttaccgc
    17041 taccttaacc acactccatc ggtcacctga ggcggagctt cgcccctttg aaataccttg
    17101 cttcttttcg tacaaccctg aaagcctcgc gctacatgtt cagagcattg gcgttagtgc
    17161 tctggctgtt gattgctttt tcatccgttt tttacatcgt taatgcgtta catcagcgag
    17221 aatcggaaat tcgtcaggaa tttaatctga gttccgatca ggctcagcgc tttattcaac
    17281 gcacctctga tgtgatgaaa gagctgaagt acatcgccga aaatcgctta tcggcagaaa
    17341 acggtgtgct ttccccgcgt ggacgagaaa cgcaggcgga tgtgcctgcg tttgaaccgc
    17401 tgtttgccga ctccgattgt tccgcaatga gtaacacctg gcgaggttct ctggagtcat
    17461 tggcgtggtt tatgcgctac tggcgcgata atttttctgc ggcttacgat ctcaaccggg
    17521 tatttttaat cggcagtgat aacctctgca tggccaattt cggtctgcgt gatatgccag
    17581 tggaacgcga taccgcgttg aaagctttgc atgaacgcat caataaatat cgaaatgcac
    17641 cacaagatga tagcggcagt aacctctact ggatcagcga aggtccgcgc cctggcgtcg
    17701 ggtattttta cgcgttgacg ccagtttatc tggcgaaccg gttgcaggcg cttttgggtg
    17761 tcgagcagac catccggatg gagaactttt tcttaccggg tacgttgccg atgggggtta
    17821 ccattcttga tgaaaatggt cataccctga tttcgcttac cggaccagaa agtaaaatta
    17881 agggcgatcc tcgctggatg caggaacgct cctggtttgg ctatacggaa gggttccggg
    17941 agctggtgct gaagaaaaat ctgccaccct catcgctaag catcgtgtat tcggtgccgg
    18001 ttgataaggt gctggaacgc attcgcatgt tgatccttaa cgcaattttg ctgaatgtgc
    18061 ttgccggagc tgcattgttt actctcgcgc ggatgtacga gcgacgtatt ttcattccgg
    18121 cggaaagcga cgccctgcga ctggaagaac atgagcagtt caatcgcaag attgtcgcct
    18181 ccgcgccagt gggtatctgt attttgcgta ccgctgatgg cgtcaatatt ttaagtaacg
    18241 aactggcgca tacctatctc aatatgctta cgcatgagga ccgccaacga ctgacgcaaa
    18301 ttatctgtgg gcagcaggtc aattttgttg atgtcctgac cagcaacaat accaatctgc
    18361 aaatcagctt cgtccattcg cgctatcgta atgaaaacgt ggccatttgt gtgctggtgg
    18421 atgtttcttc gcgcgtgaag atggaagagt cgttgcagga gatggcacaa gcagcggaac
    18481 aggcgagcca gtcaaaatcg atgttccttg ccaccgtcag tcatgagctg cgaacgccgc
    18541 tgtatggcat tatcggtaac ctggatctgt tgcaaaccaa agagttaccg aaaggcgtcg
    18601 atcggctggt gacggcaatg aacaactctt ccagcctgtt gttgaaaatt atcagcgata
    18661 ttctcgattt ctcgaagatt gaatcggaac agttgaagat cgaaccgcgt gagttttcac
    18721 cgcgtgaagt gatgaaccac atcaccgcca actatttacc gctggtggta cgcaagcagt
    18781 taggcttgta ctgctttatt gaaccggatg tgccagtggc cttaaatggc gacccgatgc
    18841 gtttacagca ggtcatctcc aacctgttga gtaacgccat aaaattcacc gataccggct
    18901 gtatagtttt gcatgtccgc gctgatggcg attatctctc tatccgtgtt cgcgataccg
    18961 gcgtggggat tccggcgaaa gaagtggtgc gcttgtttga tcccttcttc caggtcggaa
    19021 cgggcgtaca gcgaaacttc caggggaccg gtctgggtct ggcgatttgt gaaaaactga
    19081 tcagcatgat ggacggcgat atctcggtag attcagaacc gggaatgggc agccagttta
    19141 ccgtgcgtat tccgttgtac ggcgctcagt acccgcagaa aaaaggcgtg gaagggttga
    19201 gtggtaaacg ctgctggctg gcggtccgca atgcgtcgct ctgtcagttc ctggaaacca
    19261 gtttgcagcg cagcggcatc gtcgttacaa catacgaagg gcaggaaccg actcccgaag
    19321 atgtgttaat tactgacgag gtagtgagta aaaaatggca gggtagagcg gtagtgacct
    19381 tctgtcgtcg ccatattggt attccgctgg agaaagcgcc aggggagtgg gtacacagtg
    19441 tggctgcccc gcatgagcta ccggcattgt tggcgcgtat ttatttgatc gagatggaga
    19501 gcgacgatcc tgctaacgct ctgccgtcga cggacaaagc ggtcagcgat aatgacgata
    19561 tgatgattct ggtcgtggat gatcatccga ttaaccgacg tctgctggca gatcagttgg
    19621 gatcgttggg ctatcaatgt aaaaccgcga atgatggtgt cgatgcgctt aatgtactta
    19681 gcaagaatca tattgatatc gtgcttagcg acgtcaacat gccaaatatg gatggttacc
    19741 gcttgacgca acgcattcgt cagttgggac tgacgttgcc ggtaatcgga gtaactgcta
    19801 atgcgttggc tgaagagaag cagcggtgtc tggagtccgg tatggacagc tgcctgtcga
    19861 agccggtaac gctggatgtg ataaaacaga cgctgacggt atatgccgag agggtcagga
    19921 aatcgcggga atcgtaggac ggataaggtg tttatgtcgc atctggcatt cagtgcaaat
    19981 gccagatgcg acgctgacgc gtcttatctg gcctacttta atgtaatctg ttgaaataat
    20041 gggaatcgta ggccggataa ggcgtttacg ccgcatccgg cattcagtgc aaatgccaga
    20101 taagacacta acgcgtctta tctggcctac aggtgattag tctttatctg ccggacttaa
    20161 cgtcacagaa gagagataat tcagcagggc gatatcgttc tcgacaccca gcttcatcat
    20221 cgcagatttc ttctggctac tgatggtttt aatactgcgg ttcagctttt tagcgatctc
    20281 ggtcaccagg aagccttccg caaacaggcg cagaacttca ctctcttttg gcgagagacg
    20341 cttgtcaccg taaccaccag cactgatttt ttccaacagg cgagaaacgc tttccggggt
    20401 aaatttcttc cctttctgca gcgcggcgag agctttcggc agatcggtcg gtgcaccttg
    20461 tttcagcacg atcccttcga tatccagatc caataccgca ctaagaatcg ccgggttgtt
    20521 gttcatggtc agaacaataa tcgacaggct tgggaaatgg cgcttgatgt acttgattaa
    20581 ggtaatgcca tcgccgtact tatcgccagg catggagaga tcggtaatca acacatgcgc
    20641 atccagtttc ggcaggttgt tgatcagtgc tgtagagtct tcaaattcgc cgacaacatt
    20701 cacccactca atttgctcaa gtgatttgcg aataccgaac aagactatcg gatggtcatc
    20761 ggcaataatt acgttcatat tgttcatgta cttgtaccta tgtattaact atttgatttt
    20821 aaaaatatat tctatgcttt gtttagtcgt tggggcacct gtgggacata agtacatagc
    20881 tttctgttta gtacttctac ctgggactgg ttattgtctt tcatccatgc accgtaaaca
    20941 ttgtaaacca tctgggcatt cgcatgtccc atctggttgg ctatgaagtt tggatttgct
    21001 ccggcagtta aagcccagca agcataagta tgccgagact gatacgcttt tctatggcga
    21061 atttttgctc gttttaatgc agattcccat gttctgttga tggatgttac ggcataatga
    21121 actcctgagt tacctgtgcg atctaatatc tgaggattga aaacaaagct gcatgagtga
    21181 gtgcttgtcc ggccatactc tcgcagcttt acttctatct gatattgttt accaagcctg
    21241 gttaatgtcg cctgattttt taatacgtca attgctggct gtatgagttg tattaccctg
    21301 tttgttcctg catctgtttt gggtagtgta aattcctttg tctgagtgta attccttttg
    21361 acggtcaagg tacccgcttc cagatcgata tcctcccatg ccagcccaca cagttcgcca
    21421 tgccgcatcc cggtatatac agccagcgac cagaaatttt tagtttgctg gtggtggcac
    21481 gcatcaatca gtctggcaaa ctcatctttt gttagtggat cgggaggtgt tttcgctctt
    21541 ttcagtgatg gaagctcact gaaagggttt ttgcctatat acccattgtt tgccgcaaaa
    21601 cggaacatcc cggagataat tgtcatatag ctatttacag taggtacact acgaccttta
    21661 ataggcttcc tctgattttt tcctggcaac tgatatcccg ttaataattc tttacgaaaa
    21721 atgagaacat cttcctgtgt aattgatgat gccagtttgt tccctccaag gcagtcaagg
    21781 caatttcgga tagctgattt gtatctgacc atggcatttt ctgttatttc cattttctta
    21841 agctcaagcc atttttccga aagtgcctta atggttatct ctttttttcc cagaccaaag
    21901 tgtttcaggt taggggaatt agggaactgc gcggcgtagt cgaagcaccc cattcttatt
    21961 gcaaaacaaa cggaagtgcg aagttcacct gcgatcttcc ggtttttggc ggtgtcagga
    22021 acaccgaggt tttctctgac acgtttgccg ttatagtgaa accatatgcg gagtgatcca
    22081 ccatggtttt caacgcctgt cgggtatgat gcgttactca ttaaacctcc cagacgtcca
    22141 ggagcattaa caggttaacc ggaacttgca tttttggcac ctggttgttt ctggttttcg
    22201 atccagcgca taatttcctc aatgttgtat acacattcac tgaaaggccc gggctctcct
    22261 tctgctgcgt aatgacggta ttctttcccc agtaaccagg atttttttct tgctctggca
    22321 atggttcctg atttcagtcc agttgcagca ataagggttc tttctgtgca ccatttgctg
    22381 ggcgttatct gatagatgat tgtttccatt cctgactcac actatcttca ggccacggca
    22441 gtggcaccac acgtcaaaca ttcgctttac aacttcacgg cagtagaagc cgtgagcatc
    22501 tctggttaaa tcgtatcggt acccgtatct gagtcgtatc catatttcaa actcgcgatt
    22561 cacgacttat tctcctgctg tctttatgcc agttcatccg gttgcgctgg ggcccaggcc
    22621 ttccgtggcc tgtatttgcg gcgttttctg gcttcaatgc gtatctttgt caccctgact
    22681 ggctttggtt cgtgccggaa cactttgttt ctgatattac gccgtgtatt catcatccag
    22741 ataaaacgct gggtatggtc gcatccgtca tcaacggtaa ttactgtgcg gaccagttca
    22801 tcgttaatat caaccatttc acccattcac tgagccctca tacgcgaatt tcgtggcttc
    22861 catcagttgc gtccataccc attcaagaaa ttgtgcgttt tctcctgagc ggtcatattc
    22921 atcgacgctg cggggcttga tggtcgccag tagtggcaca atccggtact tcatctgttc
    22981 ggttaacttg tccggtacaa gtcgccagcc gttatatttc gatggaacat ggtggtattc
    23041 agccaggaag cgtcgtatat cgtttttcat ttcctcgcat gagttctgat catgaagtgc
    23101 agcggaaagc cagcgtccga tatattcagg gattcgcaca tcaagtactt tttctgccgt
    23161 cgtcagcatt gcctgtcggc aggcgttcca gccgttgaca tagtgacgtg ttgcatccca
    23221 tgaaacctgc acatcagggt aagcatcctg ttctgttctc actggcggta tctgaggaac
    23281 agtgaatagt ggactgacga tatatttatc cagagagcct ccccgctctt ctgcaagact
    23341 ttcccacgat gtcattgtgg ggaccctgta acctgctttt ttgtgcgtat acatccatgc
    23401 cactctgccg ttatcatctg cggcggcaat gcgctgccag gcaggataaa gcggggtgta
    23461 tgtttctctg tcattacggg cgtcaggcat ggcatcaata ctgcaaactt cccccgtaag
    23521 gttattcagc caggccttcg ggctaatctc tgacgttacg atttcatcca gttttttctc
    23581 cagattttct atgtgactga ttagcgcaag gataactggc ggagtggcaa gggagtgaaa
    23641 tgcttccaga tcacatccct tgtctccata tacggtattt tgtgcggcgt cacgcagtgc
    23701 tttcagatca gcttctttca tcgttcagac tcccttgttg ctgttttgta ggcgcgcagg
    23761 atgtcgcgtg tttttcctga taatgacgac ttaaccagga aaaagccgct ccggctttct
    23821 gtgatgtccg gcgtgcacag aagtaccgca tcaacgacac gattatgttt tcgcaatgtc
    23881 aggcgggaac tggtgataat cagttttgcc tgttcgccgt aatcggtgaa tgaaataatc
    23941 atctgatggc ggtctccgtt atgaaagtgt tcctgctggt tcaatgctga taatttctgc
    24001 tgcaataagc atacctgcca gccatgcttc gccgagcatg tcctcatccg tacatgcaag
    24061 atcaccgatc tgtattgtgg ccataatgtt agtttcaccg gtatattcat cctgtgcttc
    24121 ttcatacggc aggttttcat aaaggttttc aattgcgcag gttattacat ccagtcccgt
    24181 cagattgccg acaacgtcca cttcaaatgt ttcgcggtac tcccattgcc cgagtgtcag
    24241 acgaaccgtc tgtcttgcca tgcgcccgca taatgtcatg ttcgggtcgt agttcatgat
    24301 tgccgcagta gtgctattca tgttctgcct ccggtgtgta aatagctttg tcgtgtttaa
    24361 attcaccgtt ccagtttttc ttcataggga gttcaccgtt catgtaaaac tggtataaac
    24421 ggtggcatcc tttttccagc aatacggggg tgaatttcgt gaatgttgct ttcccgtgtg
    24481 gagtgatctg tactgcttct tcggtcaggt atctgtcacg ggcataagag gccgtgcgcc
    24541 agcggggtgt tttctccgga tcgcgttgtt cgttgaatac ccattttcgg ccagacagcc
    24601 accacattat ctggctggta tttacgccgt tcagcgcacg gcagaactgg ggaacagtca
    24661 tgcctttaga gaaatgtttt tccagactgt ctacggttgc agagagcgtt ttattttcca
    24721 gggccatagc ttcagcccgt tcttcggcct caataaccat caacgcgagt tccttgcgag
    24781 tgagtacgcg ctgaacatcg atgttttccc gctgagtgaa gtagaactcc acaaggtctt
    24841 cgtgatagtt ccacgcctga tccgtttcca gcaattttgc gtggttggct gcgccgcgtt
    24901 cggtccagag gatgagggat cgaacattgc tggcaatttt cacagagtag ctttgagata
    24961 ctctgtgctt aagttcacgt agttcatcgc cttccagcaa aaaataatgc tttccaacaa
    25021 cgaatcgttc ttcgtttcgg gaatgattct ggcgaatacg gattggtgtt gctccataac
    25081 ctgccgccag ttgttcagtg gtcacaacgc gttgaccgcg atattcgata atttgcaaat
    25141 cacgggctgc caccggcaca agttcagttg attttttcat cattacacct cacgaaaagc
    25201 tgattagtgg aacactctgg tttccggttc tgagttgtca gtggcttcac tgataagccc
    25261 atccagaatc atgtgcatcg ctctgtaacc ttccattgtg atgccggttt tttcgccgtc
    25321 gcccagagcg ccggtgaatg ctttgtacat aagaatgctt ttgagcagcc ctgattccgc
    25381 tccgtaagtt tctatgagtg cccattccat atagttctgc atagcgagac gtaatggttt
    25441 caggtagatt gtcatgccac cgttgtggcc tctgtacatg gtgcccttgt ctgtaccgcc
    25501 attctcgtta agcacttcgg tccagccgtt tctggtgagc tgttcagaga agaacatggc
    25561 ggtataaagc cagcgccagt acatgattgt cttttccggc gcgagctcac ctgtcatgcc
    25621 tttttcttct gcttcactga tgcaacacac tatttccaga ccatgccaga tcggctcgtc
    25681 aaaacagcct tcatcgagca tttccagtgt tttgttgata tcaagcagtc cgtaaggtgt
    25741 gtggatacct tcagcggtaa gaaaaaataa ctgttcagtt gcagtcatgg tttgctctct
    25801 tcttaaatgg tctttgcgcg acaagccggc aatactgatc aatccaggcg ttgtattttt
    25861 ccatccaggc atcaaactgt tttctgcgtg caaggatgcg caacaggcga tgaaaacaac
    25921 gcccgtgagc tctgtagtat tcgggcgtaa tttctcccgg atgaaagact tctccatatt
    25981 ccggatgcat caggcgctgg tcaggcagac gtttttcaaa acctgaactt tcgaatacct
    26041 tcgttgtcat aaaatgagcg agatgtatga tggccgtttt tcgcgtgaag catttcttaa
    26101 cttgtccgtg gcggcagact ctgtaaacag ggccgcaatc gagttcgtat ttatgaaaag
    26161 cacggtcgat tccggcatta tttattgcac tgtattccgc atcactgaag aaaggtggaa
    26221 atacgatgta tccttccatt gtcagcgcca gaacgcgcgg atcggtttct gtcagccatt
    26281 cgttttctgt gaattcttca gaattaattt caatgatttc aggttcttct ttcatggtta
    26341 ctccgtgttc atccggtgtt aattactttt attcactctg agcgcagcca gaagatgtgg
    26401 ctcagcaatt cgtttgttgc gatatatctg tgatacagcc tggccttttc aggttgtaaa
    26461 aatccccgac aataaaactg taaattaatt actgttctta aataatgtta ttcgttttca
    26521 gtccggcaca gatgcttttc tgctttgtct gctgtacagg ataatgagcg gaaaagacaa
    26581 ttcatcatgt ttagcgcatc tggtgaacca atatcaccgc tgtcaataat cacctgtaac
    26641 aaagacaggc tttctgttat ctcgcagtgg acattatcga gtacttcaac gcaattattc
    26701 atttttcatt atctccgtta ttccggggga ggagctatgc tgttaatgat taacgcatcc
    26761 ggtaatatgc ccctctttaa tgtaatcgat gcattcattc aaaagttcat caatgattaa
    26821 ctttcctgat tcagtcagat actcaccatg ttggttgata ccgacagcat cctggtatgc
    26881 agtgcgtatt gctgtttcac catctttccg cccaaactca ccacgggtaa cgccttcaaa
    26941 tcgtaatagc aactggctta tgaactgttc tgttatttca atagttgtta actctccatc
    27001 atgacgcttt acagtgagga gattacttcc tgtttttcgt ttcatccgcc gcagtgctgc
    27061 aaccgttatg cggcgacgat atgcagaaat gtattcatcc ttcatttgtt atttcccgta
    27121 tgcctttttg agaaacaata ctgcaactga ccagtatccg gcatcagcca ttaataaagc
    27181 ggttttatag gcatgtttgt ttttcatgtg tcatccccat tttaaaatag tgagaactcc
    27241 cgaccgcaga ccgtcatgtt tttatgtatg attgtctgtt taatttcctt tattcgttct
    27301 cagagcatca acatactcgt aagccttttc acaggtttta ttcatggaac gaatcagaca
    27361 aagtaagaaa tcatctgtct ctccgttatc accagagttt ttaaatataa actcaagcat
    27421 tgacgtgttt tcttttattt ctgctgccac ttcctcaagc ataatcaggg gagttttcat
    27481 gttctttgtt ccttaaatgc atcgcatgcg cttctggcgt attgttgtgc cagtaaaaat
    27541 atgtcatccg aaagttcatc acattcttca tcaccggaag ccgaaatgat taaccccgct
    27601 tcaagcagta ctgcaatgtg atgaaaagct gtttccggtt cgttggtgag gcctttgaac
    27661 attttcatct tacgattcct ttgtcgtgtc agaatggaaa cttgtaagta accacatcgg
    27721 atcgcagtca agtatatttg ccagaggaat cacctggctt gccagaggtt cagccttgcc
    27781 gttctcccac tggatgatgg tttcttcatc aactccaagc agcgtggcga gttcagcggt
    27841 ggatagaccg cagctttcgc gttgggtgcg gatgccatca cggcaatctt ttttgtttct
    27901 cggaggtaac agaatatgtt ttagttgttg gattttttcg attccaacct caaggagtac
    27961 gtgacggttt ctttccgata cagactccag actttcatga agcagcgatt ctacttgttc
    28021 gataagcatc aattgtgaag tagacataaa aaaacctcgt aaccaatatt tcgaaatcta
    28081 ggttacgagt gagtaatgta ggtgtcaaga gcaattaatg ttttttgttg aattaaattt
    28141 gcatacgcat ctcaacgaca acaccaataa tttcacaatc cccattgatg gggatcagtg
    28201 gatagcgtgg gtttagtggc tttaggaaac attgcccagc gtcttctatg tactgtttaa
    28261 gggttgcctc tccggagttt ttgagcctgg cgacgacata ttttcctgaa aaaacttctt
    28321 tgtcagggtt aacaagaatg ttcatgcctt caaaaataga gaactctcca ttctgtgatg
    28381 tcattgagtc ccctttcact tccagccaga agccgtgatc gcccgcatat atatcgctac
    28441 caatccattc ttctggataa gctatgtaat cttcgcagtt ccaggggcca gcactcaccc
    28501 atgttagttt cggatatcta taacggtttg gagaaactgg cttaggattt cggacgtttt
    28561 gaatgctatc tgtaggtgat ggggcgctgc cgtcaagaag ccatttagga gagcatttca
    28621 atgcatccgc gattctaaat aggtttgaag atcttgtgtc ttgtgagtct cctaactcta
    28681 ttttgctgat tgttacacgc gagacaccag tcaattttgc caggtcatct tgtgacattc
    28741 ctagctgtac tctgcgcatt aacagtctgc caccaaagct atcgtgatcc atcttctccc
    28801 ccttcttgtc gaaataatcg tttcgaaaca aatatagcaa aaaatcttga aatctaggtt
    28861 tcggaaaatt aagattaatc gaaacaaacg tgtcgaggtg gatatggatt tgtatgaaat
    28921 tctaaaaaac atgtttggat cgaatgttga aataggtcgt tattttcccc gaaggggaag
    28981 ggctagaacc ggacaggctg taggtaagtg gaaaacccga ggtgtgccgg aagacgtggc
    29041 gatcctttgt catctggatc ccaaaattcc ttatcagcat ccgtcgctca tgaatgccag
    29101 tcatgaatcg tgatgttctg ccggaggctg tatgtcccct gattacgttc agatggagat
    29161 gcctgccagg tacagccagg cagattcaga gtggattaag cggcagttac agagcctgcc
    29221 ttcctcactg agacagaagg ttgccctgaa atacgcggag gtttacgaag ttacttttga
    29281 ttccgagcct gtttcattcc gtaaggagaa cagagcgagg catgaagcca atgtacggct
    29341 gcgcaggttc gttgaaacac acgggcgtgc attacagggg tatacgactc agccgcccct
    29401 ggcaggaacg caacagcgta cctgaatggt gttggtctta aaggtgaccg acgggctgag
    29461 accccacttc tcaggatgca cctgtgtact accaagctag tgcataagtt gagagggggg
    29521 taaggggggg agtgcccgtg tgttagtgcg aagcactgga acgggatctt ccaacagacg
    29581 ggtacagagg ttaggtagat ctcgatctaa aggggcatcc ctgaaaaaac ggacgtatca
    29641 gcaaactagt acatcatggt taaaatatga gcgctgaact gaaacaggta ctaatcaacc
    29701 ttctggaaga ggaattcgga catggaatgg acaaggtcac attcgactac gttctgcaaa
    29761 agaaaatcaa atctctgggc tgtgagttgc aacgctgttt cactgtcagg ctgaaaggtg
    29821 atcgcagggg atttatcgat cttctggtta tttctcccga tggtcagcgt tgcgcagttg
    29881 aggtggataa ccgatcgccc cgtcagcgtt cactgatgaa aattcgagcg cttcctgaag
    29941 gcatttcggg atttgttttt ctccgtgatg gcaaacatcc acagcgttat atcgatggtg
    30001 tggatgtcat ccgggcgacc cggtttaagt aatcactgta tcagccctgt catcgggaga
    30061 ataccatgct gaatatcaag ccgaattttg ctcaggaacg tgcgctgaat atgttgcgtc
    30121 atgactggaa aacatatcag tccttcatga tgtacatgcc tacagggagt ggtaaaaccg
    30181 gcctggcggc gttcatcact gctggtctgg taagccgtgg catgcgcgtt ctgtttgtcg
    30241 ttccttatac cattttgatc aaccagacag cacagcgctt tgttcagtat gggctttccg
    30301 gggatgaaat cagctttatc tggcgtgatc atcccaataa cgacccgtca cgactgattc
    30361 agattgccag tgcggatacg ctgattcgcc gtgattttcc tcagaatatc gacctgctga
    30421 ttattgatga agcgcatctc cgtaagcgcc gcatcctcac ggaaatcgaa cgactggttg
    30481 cagagggaaa tgtgaaggtg attgggttgt ccggtacacc cttttcatcg ttcctggggc
    30541 gttattacca gcgtcttctc aagccgacca ctatagggga actgatccag cgtggcgaac
    30601 tgagcaatta cgaattttat gcgcccacga aaccggatct ggaaggtgta aaaacgaaag
    30661 tctcaatgga gtacggcagg gattacgacg aatcacaact ggcagagatt atgtgcggcg
    30721 cggatctggt tggcgatatt gtcgataact ggctgtgtaa cggaagggat ttaccgacag
    30781 tggcgttctg cgtcaataaa acgcatgcca gtttcgtgac catgcagttc aacaaagccg
    30841 gaattaacgc agaggtgatg gttgctgaaa cgccacatga tgagcgtcag ttaatgatca
    30901 gtcgttttga aacgggagcg acgaaaatca ttgtcagcgt tggcgttctg gtggcgggat
    30961 ttgacagtga tgtccggtgc atcatttacg cacgcccgac aaaatcagag attcgctggc
    31021 ttcaggcgat tgggagagga ttgcgtacag cgccaggcaa ggagtcatgc ctgattttcg
    31081 accattccgg tacggttcat cgcctgggat ttcctgaaaa tattgagtat gacgaactgc
    31141 ccggcaaaaa cgacgggatg caggtgtcat catcacgtgg taacgaaatt cgggaagaaa
    31201 aactgccgaa agaatgtccg gggtgtcatt tcatgaaacc ggcaggtgtt tatgtctgtc
    31261 cgaaatgcgg atttaaaccg ctggctggtg aggatgtgaa cacagatacc cgccgcaata
    31321 tcaggaaact cagcaaggac gaaaaaattt acaccaaaag cgacaaacag gcctggtgga
    31381 gtcagatcaa attctatcag cgtcagcgtg cctctctggg gcgaccggtc agcgatggat
    31441 ggtgcgctca cactttccgg gagaagttcg gcgaatggcc tgacgggttg agcagttttc
    31501 cgatggaaat caccccggag gtcagtaatt acatcagaca caaattcatc cggtttgcca
    31561 gagggcgtag ccgggcagag aagatgacgg aaaaatcccc cgaagcgctt tctctgtccc
    31621 tgcttcatgg tgtccggtct ggggtgccgg aaggcagtga gtcgtggcag atcatgcagg
    31681 caaagcaaca acttcagaaa aacagaaaca gtctgagtca gtaagatgaa aacagcagaa
    31741 gcagcgaaag gccactggcc tgaaatttta aaacattacg gactgccgcc agtcacgggt
    31801 aaaaagcact tcaaagggga atgtccggtt tgtggtgcca gaggtaagtt ccgtattgat
    31861 gaccgtgatg gcaccggaac gtggatctgt gtttgtggta gcggtgacgg catgaaactg
    31921 atcgctctga cacaaaaaag agcgtttcat gaaatttgcg ctgaaataga ccgcatcacg
    31981 gggaatgaat accggagaga taaaccgcct gtgatctgta cgtcggaaag cctgagatcg
    32041 cgcgttcagc gtcgattttc agcgctcacg gcgctacagg gaacatctgg tgctgaatat
    32101 cttcgttccc gtggcatatt cagtcttccg gttgagggag ttcgcttcaa cagccagcaa
    32161 aattataacg ggcgcatgtt ccagtctctt tatgccctgg cgaccgatga caagggagaa
    32221 ctgtgttacc tgcatcagac attgcttgat ggagctaaaa aagcagacat tggtatcagc
    32281 gccagaaggc tcaaatcttt acaggacgat aactatctgg accatgcccg gtcagttgct
    32341 atcaggatgt ttccggtagc cagtacactg ggtatagctg aaggtatcga aaccgcgttg
    32401 tccgcacatc agatttacaa agtaaatacc tgggcaacca ttaacagtgg ttttatgaaa
    32461 aagttccgcg taccggcggg agtcacccat ctgattattt ttgccgatcg cgatgaatac
    32521 agtgctaccg gaatggctgc ggcctgtgaa tgcgcacatg caaatctcca ggcccgaaat
    32581 gacattcagc gggtaagtgt ccggtggcct gatcatgatg atttcaacaa tatgctcatg
    32641 aacggcgatc aggttcggga actggttttt tacagaaaaa aggcggttgc gtgatgcgta
    32701 cggacaatca ggtacataaa gctttattca ctatcccgac ggcagcgtac agcgccgttc
    32761 cggcaaatat caaacctctg ccagaacaaa ggaggatcac cggacataaa cagactgatg
    32821 cttatctctg gattctggag gttattcacc tgaacgaggc cgtacatctg gacgcagccg
    32881 aagcagcact ggagaaactc aaaataacgc cggaagaagc gagtgaacgg tatggacgtt
    32941 atttgcagga gattaatgtt gatcctttcc agattgcttt cgcaaccata ggcatggata
    33001 acccggcgca ggcgatcagg aatgcccgtg agaatatcaa aaaagccgca tcagtcaggg
    33061 ccacatttgg cagctatgag gcagcactcg acgatgtgga ggctgagcgg ataattcgca
    33121 cctccccgaa atttatcgac gattactact ggggctggac tgcggcagag aaaaaagccg
    33181 gaagtattga cggtgttcgc tcaaatgaaa ttgatgatca gcgtcgtgca tatgttgatg
    33241 gctattgtga tgtactgcca gagcctcata cattgtcaga cgttgttcgt gagtttattt
    33301 actgggactg gctttatgag atgcgccaga cagcggggag agaaacaggg gacaaatacg
    33361 gtttcacagg tgaacatcat gagtcagtat atgatcggca gttctggctc gaaaatttgc
    33421 tgggaaaaat aaagcctgtg acgcgtgatg aagcggttga agtgtgtcgc tggtttctgg
    33481 caagcggaaa agatgagtac atggaagaca atggttcagc ggtcattctc aatctggttg
    33541 gggagtgtga gcaatgaagc cggagatatc cagtagacat tttccccctg agcattcaaa
    33601 tcagaaaaag tatggttaca gcggagtaca gaataatgcg tgatatccaa caggttctgt
    33661 cccgatgggg tgcatgggtg gtaaataatc atgagagtgt ggcatggtcc ggcgtcgctg
    33721 ccggatttaa gggagtgatc ccatcaaagg ttaaatcacg gcctcagtgt accgatgatg
    33781 atgcgctgat tatcagcaat tgcatggcgc aactgaacgt caacaacagc gatttgcatg
    33841 attttctgta tgattattat gtgttcggga tgacgcttat gggactgggc cgtaagcatg
    33901 agcgctctga ttgctgggcc gggcgggtac tgcaaaaagc tgaaggtgtt attgagggga
    33961 tgttgattat gcagggaata aaactggaaa tggacagata cgttgagcgt gaacgatcag
    34021 gggcacaggt cagtcagttt tccggacata cgggaaattg aaagcgcggg gttttactgt
    34081 agaatgactg cgggtgcttg agggtgtctg cctcgggcat gctgccgtaa ggcagacgga
    34141 gaaaagcccc agttaacatt acgcgtcctg caagacgctt aacattaatc tgaggccaat
    34201 ttcatgctga acacatgtag gttagcctct tacgtgccga aaggcaagga gaagcaggct
    34261 atgaagcagc aaaaggcgat gttaatcgcc ctgatcgtca tctgtttaac cgtcattctg
    34321 acggtactgg tagcgaggaa agacctctgc gaggtacgaa tccgaaccgg ccagacggag
    34381 gtcgctgtct tcgtagacta cgaatctgag aagtaagaga cctggcgggg gagtaatctc
    34441 ccgccccctc tgatgtgtca ggcatcctca acgcacccgt actttagttg tcatatgctc
    34501 cgcaaaatgc aggttgtcgt tgcaacccgt gcggtaattt tttgtgtgcg agtgctcaaa
    34561 aaacgttgat tttcataaaa ggacgatttt atgctgcc
//
LOCUS       DAZGXZ010000044        33952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000044 DAZGXZ010000000
VERSION     DAZGXZ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.044"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHKJF_002192"
     CDS             complement(119..361)
                     /gene="yiiF"
                     /locus_tag="ACHKJF_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFP0949548.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHKJF_002193"
     CDS             complement(580..798)
                     /gene="yiiE"
                     /locus_tag="ACHKJF_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0949549.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1384..1674)
                     /gene="nadS"
                     /locus_tag="ACHKJF_002194"
     CDS             complement(1384..1674)
                     /gene="nadS"
                     /locus_tag="ACHKJF_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP0949550.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1675..1986)
                     /locus_tag="ACHKJF_002195"
                     /pseudo
     CDS             complement(1675..1986)
                     /locus_tag="ACHKJF_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2215..3123
                     /locus_tag="ACHKJF_002196"
     CDS             2215..3123
                     /locus_tag="ACHKJF_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0949551.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3187..4128)
                     /gene="fabY"
                     /locus_tag="ACHKJF_002197"
                     /gene_synonym="yiiD"
     CDS             complement(3187..4128)
                     /gene="fabY"
                     /locus_tag="ACHKJF_002197"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP0949552.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4173..4610)
                     /gene="dtd"
                     /locus_tag="ACHKJF_002198"
     CDS             complement(4173..4610)
                     /gene="dtd"
                     /locus_tag="ACHKJF_002198"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP0949553.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQVGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4607..5479)
                     /gene="yihY"
                     /locus_tag="ACHKJF_002199"
     CDS             complement(4607..5479)
                     /gene="yihY"
                     /locus_tag="ACHKJF_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP0949554.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5473..6072)
                     /gene="yihX"
                     /locus_tag="ACHKJF_002200"
     CDS             complement(5473..6072)
                     /gene="yihX"
                     /locus_tag="ACHKJF_002200"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP0949555.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6213..7100)
                     /locus_tag="ACHKJF_002201"
     CDS             complement(6213..7100)
                     /locus_tag="ACHKJF_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0949556.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(7090..8439)
                     /locus_tag="ACHKJF_002202"
     CDS             complement(7090..8439)
                     /locus_tag="ACHKJF_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949557.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(8538..9971)
                     /locus_tag="ACHKJF_002203"
     CDS             complement(8538..9971)
                     /locus_tag="ACHKJF_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949558.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(10041..11051)
                     /locus_tag="ACHKJF_002204"
     CDS             complement(10041..11051)
                     /locus_tag="ACHKJF_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP0949559.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(11084..11971)
                     /locus_tag="ACHKJF_002205"
     CDS             complement(11084..11971)
                     /locus_tag="ACHKJF_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0949560.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(11996..12874)
                     /gene="yihU"
                     /locus_tag="ACHKJF_002206"
     CDS             complement(11996..12874)
                     /gene="yihU"
                     /locus_tag="ACHKJF_002206"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP0949561.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            13047..13943
                     /locus_tag="ACHKJF_002207"
                     /pseudo
     CDS             13047..13943
                     /locus_tag="ACHKJF_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            13983..14771
                     /locus_tag="ACHKJF_002208"
     CDS             13983..14771
                     /locus_tag="ACHKJF_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0949562.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14882..16147)
                     /gene="yihN"
                     /locus_tag="ACHKJF_002209"
     CDS             complement(14882..16147)
                     /gene="yihN"
                     /locus_tag="ACHKJF_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0949563.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(16249..17229)
                     /gene="yihM"
                     /locus_tag="ACHKJF_002210"
     CDS             complement(16249..17229)
                     /gene="yihM"
                     /locus_tag="ACHKJF_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP0949564.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(17237..17947)
                     /gene="yihL"
                     /locus_tag="ACHKJF_002211"
     CDS             complement(17237..17947)
                     /gene="yihL"
                     /locus_tag="ACHKJF_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0949565.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(18164..19987)
                     /gene="typA"
                     /locus_tag="ACHKJF_002212"
     CDS             complement(18164..19987)
                     /gene="typA"
                     /locus_tag="ACHKJF_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP0949566.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            20360..21769
                     /gene="glnA"
                     /locus_tag="ACHKJF_002213"
     CDS             20360..21769
                     /gene="glnA"
                     /locus_tag="ACHKJF_002213"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP0949567.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            22088..23137
                     /gene="glnL"
                     /locus_tag="ACHKJF_002214"
     CDS             22088..23137
                     /gene="glnL"
                     /locus_tag="ACHKJF_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP0949568.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            23149..24558
                     /gene="glnG"
                     /locus_tag="ACHKJF_002215"
     CDS             23149..24558
                     /gene="glnG"
                     /locus_tag="ACHKJF_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP0949569.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWLGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            24670..24780
                     /gene="yshB"
                     /locus_tag="ACHKJF_002216"
     CDS             24670..24780
                     /gene="yshB"
                     /locus_tag="ACHKJF_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP0949570.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(24970..26343)
                     /gene="hemN"
                     /locus_tag="ACHKJF_002217"
     CDS             complement(24970..26343)
                     /gene="hemN"
                     /locus_tag="ACHKJF_002217"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP0949571.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHCAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(26532..27041)
                     /gene="yihI"
                     /locus_tag="ACHKJF_002218"
     CDS             complement(26532..27041)
                     /gene="yihI"
                     /locus_tag="ACHKJF_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP0949572.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(27160..27326)
                     /locus_tag="ACHKJF_002219"
                     /pseudo
     CDS             complement(27160..27326)
                     /locus_tag="ACHKJF_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27623..28255
                     /gene="yihA"
                     /locus_tag="ACHKJF_002220"
     CDS             27623..28255
                     /gene="yihA"
                     /locus_tag="ACHKJF_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP0949573.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(28637..31423)
                     /gene="polA"
                     /locus_tag="ACHKJF_002221"
     CDS             complement(28637..31423)
                     /gene="polA"
                     /locus_tag="ACHKJF_002221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP0949574.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            31787..32719
                     /gene="yihG"
                     /locus_tag="ACHKJF_002222"
     CDS             31787..32719
                     /gene="yihG"
                     /locus_tag="ACHKJF_002222"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0949575.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(32760..>33952)
                     /locus_tag="ACHKJF_002223"
     CDS             complement(32760..>33952)
                     /locus_tag="ACHKJF_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFP0949576.1"
                     /translation="LNIKPGQKVVFDVDVEHGPLPITMLMHGNVIPALAAAKVNLVNN
                     ELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAPAEYGKFSFGEGQFTFNGDGSSL
                     SNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLEEKKFPVGESESKFNQINIINHG
                     EDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLTKGNQQLGSGEWSLIAESIDPSA
                     VRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAALFKEYLPLLQKSEPTIKQPVRWK
                     NALGELNANLDISIADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAE
                     KAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAG
                     RFVH"
BASE COUNT         8567 a         8702 c         8065 g         8618 t
ORIGIN      
        1 aataaacttc aatatattga atttacatga ctttgtaggc ctgataaggc gctcacgccg
       61 catccggcat gaacaacgcg cacgttgtca gcaatctgat tgacatcatt ttttcccatt
      121 accattcctc gcgcagctta cgctgatatt cgacaccatc ttcctcacac ccttgccaga
      181 tgccaggaac actggttctt gctgtttgcg attgatttat caggtattga tctaccgctt
      241 cccttaatag gtccgcacga ggaagattac gctgcacctc gagatcatca agttgcttaa
      301 tcacctcatt cgataaatcg agtaaaattc tgcccatatc tatacctgcc agagagttca
      361 taaatatctc gccaatatca tttttgaatc tatggagaga aaagtaccct tgccgaatct
      421 ttaaagaaag ctcatttacg catcattttt tatttttgcg aggcgttttc cagatacgat
      481 gtaacagttg cagttattat aagttcaagt aaaacagcct tgtattaata ccagagtaag
      541 ggcatctgtt tttttatatt caagaatgaa aaattttttt cattccttat gctccttaca
      601 ctgatcatca acaccaacta cctccccctt ttgcctgcct ccctttgctt tacgcaccgg
      661 aaattgctgc cgctccagat attgctcaac cgcttcgcgt aaaattgcag atcgtgaaac
      721 cttacgccag ccccgaagtt tgttcagccg tttaattgcc tcttctgata aatgaagaaa
      781 aaccgtattc atcgccatta ccaccacctt acctatatta ctcattaatg tatgtgcgaa
      841 aattacgccg gaaaatcatt taaaaaaagc gggtttttgc atgttgtttt gtttttgcga
      901 ggcgttttcc agaaataata agagtgttgc agttgtttca tttattactc aaaaatggtt
      961 tagttatcgc ccaggatgaa ttatgggtgc tatgaatata ttatattcag agatatttat
     1021 ttgcataatc tgaatggtgt agccgaaaaa cgtcagcatc ctaacagcac caggatttaa
     1081 ggtgaaatta atctttcatc acagaccggt cagtactgcc accaaccggt ctttacttgc
     1141 tcatcgttat aattttgttg gtaacagttc gggaaacaag gaattccttc cggaaccccc
     1201 cggtatcatc cgatagcatt gacgtcaatc atcagcgcga cgatgagttt ttttaatcgc
     1261 atcatagggt ggcgacgttc agcacgatta cgggtcatag caaaactcct gggcaggcga
     1321 ccactatgcc gctttcacag gagttctgaa tgctagtgct ttctgtatgg cataaccatg
     1381 cctctaacgg ttaagtgctt gcagcacatg ttgcggatca ttggcaatcg ctcgcagcaa
     1441 agcctttgca ggcccggtcg gtgaacgtcg cccctgctcc cagttacgca acgtgtctac
     1501 gcttacagaa ataagatcgg caaatttaga ttgtgacaac cctgaagcgc ggcgtatttc
     1561 tttgattttc atagcatcaa cttcaaaagt ccgcgaaggc tgtcgcgttc cttcaataat
     1621 ttcattcatc tgggccatgc tgtgagtcaa ccgctcaaaa agtgccttat ccatttacca
     1681 cctcgtattt aacaaccgga gcattgcttt ttcctgggga gacagatcat ctttaatacc
     1741 cttacgataa ataagcagta atctgatctc ataatgttta acctgatgaa agtaaataac
     1801 cctgacacca gcacgtttcc cctttcctcc tgaaacccat caaacctttc ttaacccacc
     1861 agtttcaggt attacctcac catagtcagg attcaaggcc aggaaaaact gaaaacgact
     1921 gaattcatcg tcggtcagca gtttttgaac atcttcagta aaaatttccg tttcgatgaa
     1981 gagcataatt atacgccatt ggcttagagt aacaatcctt tttgtacgtc attgacgtac
     2041 ccttgaaacc actagcttac tgatagtgct ccagcaaaac cacgtctctg ccgggtttgt
     2101 ctttagattt gcgaggcgct ttccagattt ttcacgcccg tgaatttccc tgtatccatc
     2161 cagcaaaaac gggtgtataa gactgtacat tcatacagca aaataagttc tactatggca
     2221 ctggaaaaag gaattgcaga gttagtcgaa aagtttatcg ctgcgggtcg cccctcatcg
     2281 cgtcaacaaa gtattgcgca gcgcagggag ggctatatcg ccagcgctgt ccttgcgggt
     2341 gagacagaaa cgcgggtaga tattcagact atcgaacttg aagggatgac cttacgaata
     2401 gtgtcgccgc tgaatgcccc gacattactc ccgacgataa tttattacta cggtggctgt
     2461 tttgttagcg gcgggttcgc aactcacgac aaccagctgc gccagttagc ctattacggg
     2521 caatgtcggg ttattgccgt ccagtatcgg ctggcaccgg aacatacttt ccccgccgcc
     2581 catgatgatg cgcaacgagg tgcagaaata gtctggcaac acgccgaacg actcggcgtt
     2641 gataaacaac gtataactct tgccggtgac agtgcgggcg gacatctggc gctggtaact
     2701 gctttgcgat taaagcgtgc tggcgagtgg caaccggcac aactcatctt aatttatcct
     2761 atgcttgatg cgactgcgca tttcgaaagc tacatacgca atggtcacga ttacatcatc
     2821 acacgcgaca ctttactgag cggctttgag atgtatctgc caggcattga acgcaggcat
     2881 cctgaagcca gccctctttg gcgcgatgat ttcaatggct tgccacctgt acatatcatt
     2941 accgctgaat acgacccact ttgcgatgaa ggtgaagcgt tgtatcaccg catgacaggg
     3001 caaggagtgc aatgcacctg ccaacgctac cttggcgtta tccacggatt ttttcagtta
     3061 ggtggtatca gcgaggccgc gcgatcggca ctacgggatg ttgcctggag agcgggaagg
     3121 taacttcgga cctgggccct gaaaaggttt tcagattgaa aaccttttca gtcaagtaaa
     3181 acgcccctac tcttcttcgt tcccgccctc ttcatacggg ccaaatggct tcgcgggcag
     3241 aacgatatac gtgccttcaa acactgcacc cggcgtctcg tcgccaaaga tttcaacttg
     3301 catctgcacc cgtgcttttc gtccgcgcgc cagacggtcg agatcgccgc ttaaggcacc
     3361 aaggtcggct accgcatgag gtttaccgct aatcggcttg ctgtagcgga tatgcgcatc
     3421 cgccagaata atcgttccgc cgagatggcg ttcgcgcagc atcagccaga taagccccca
     3481 accggtgagc gtcgccagcg agaataaact cccggcaaac agcgtatggt gcggattctg
     3541 attgccggtt tccggcatgg tggtgataaa tttttgcccg gtatattgtt gaatacgcac
     3601 gcccattttt tcactaagcg ggatatgttc gtaccacgcc tgttgcagct gcgcgcacca
     3661 gtcgccgcga tgcaaaatat catccagagt ggcgacgggc ttaatcatca aaaaatggcg
     3721 aatcggcgtg gtggttggcg tggtgatttc tccctgatta acaaacccca gcttggcgaa
     3781 aaactccacc gcgtcttcac gggcgctaca ggtcacgcgc ttaacgcctt cctgacgcgc
     3841 caccgactcc agggtcatcg ccatcagcgt tcctaaccct ttgtcctgca cgtccgggtg
     3901 aacggccata aagcgaatgg acgcttcatt gtcggcatta atatacagtc ggcctaccgc
     3961 caccagatta ccctgctcgt cgacgaccat ctggtgatgc gccatcgcat cccacgcgtc
     4021 gcgttccgaa ccttttggtt gatgcagggg cttacgcaac atttcccagc gaaactgata
     4081 gtaacgctct aattcttctt ctgtttgtgg aacccgaagg tgatacatag cgatactctc
     4141 tcttgtttcc cgtgacaacc ctggaagctg gctcatacct gcaaccagaa tgtcacgggg
     4201 ccatcattga ccagcgatac ctgcatatcg gcagcgaagc gtcctgtttg cgtgttcatc
     4261 tcttgctggc ggcagcgttc gacgaaatag tcatataacg cctctgcgcg atccggtgat
     4321 gcgcctttgg agaaacttgg gcgcatccct cgttcggtat ctgcggcgag ggtaaactgg
     4381 gaaaccacca gtacactgcc gcccacctgt tgcacgttga gattcatctt gccttcggca
     4441 tcgctaaaga tgcggtagcc gagcacacgc tcgcacagac ggtttgcttt ctgttcgtcg
     4501 tcatcctttt cgacacccaa taacaccaaa agtcccgcgc caatttcgcc cgtcacttct
     4561 ccctccacgg tgacgctggc acgggttacg cgttgaatta atgcaatcat ggttcgtcgt
     4621 cttcttcttg ttcagctgct tgttttagtt tgcggtattc cccgagagtg acagtaattt
     4681 ccgcgccaag caagacgata caccatgtcc agtagaccca aacaaagaga atggggatca
     4741 ccgccagcac gccgtaaatg agctgatatg acgggaacat ggtgatataa agcgcgaaac
     4801 ctttctttcc tgcttcgaac aggagtgcag cgacaaacgc gccgacaatc gcgtcgcggt
     4861 taggtacgcg gatggtagga acaatgctgt acagcaacca gaaggagatc cacgacaaca
     4921 gcagcggaaa aatacgcagc acgttgtcga tgacagtatt gagatcgctc gcccagcgca
     4981 gggagagcaa ataggaactg atcgccagac tggcccctgc cagcagcggc cccagcgtta
     5041 aaatcatcca gtacacggca aacgagtaaa tttttggtcg cgctcgttta ctgcgccaga
     5101 tggtattcaa cgcgctatcg atggagtaca tcaacaataa tgcggtgacg atcagcccgc
     5161 acgcgccaac ggcggtcatc ttgttggaat tggcaacaaa ttgttcgata taccgctgaa
     5221 taacatcgcc agtagcaggc agaaagttgg caaaaataaa gtgacgcaac tgaatgctga
     5281 cgtcggaaaa catgggaaaa gcggcgaaaa gcgcaaaaac aacggcaacc agcggcacta
     5341 atgagagcaa agacacatag gcaaggttac ctgccagggt tgtcatgttg tcctcatcaa
     5401 tacgttgcca gagtagtttt agccaggccc atagtggacg agtacggtgc ctggctttgt
     5461 cctgaatggt ttttagcata acaccttcgc gaaatagtcc gggatggtgg ttttatcttt
     5521 caccagaata ctggtaatgc ccagctgatt ggctccttct atattatcgg cgttatcgtc
     5581 gaaaaagacc gtatcgctgg gtgaaaaacc ttccgcctgc aaaacatgct ggtaaattcg
     5641 tgcttcaggt ttgcgcatcc ccagatcttg cgacagatag atatggtcag cagcatcacg
     5701 aatttccggg tattcttccg gccagaaggt ggtatgcagg cggttggtat tggaaagcac
     5761 caccacgcga tgcccctgct cacgcagttt atgcatgatg gcgatcactt ccgggcgcag
     5821 cgcaacaaac accgcctgcc agccgtgaga gaactgctcg tagcttagcg gtagagccat
     5881 ctcatgacac agcgcctctg cgaacgcttc gtcgctaatt tccccacgct catgctgatg
     5941 aaacgcctcc cccatatgaa aactcttctt aagcgatgcc agcggaatac gcgttaaatc
     6001 gctccaggct cccagcacac ggttaaagtc gatatcgaca atcacattac ctaaatcaaa
     6061 gatatagagc atttttgcct cctttcgcgc cacaagaaat taactgtagc gggaaaggat
     6121 gggtttggct atgtggcaat aagggtaatc ttgtgatgct gattgcgaaa aagcagagta
     6181 atttcactgg ctttttcgca atagatgaat ggttaagatt caggtgtaaa ttgccaacga
     6241 gtttcgatga ccagcggcct atctggtgtc accaattccg gcgttagatt tattgcgtta
     6301 ggtgcctgag tcagcgggtt aatacaggtg gcatcgggct gtttatcaaa taccaccaga
     6361 ctgtttgcag aagagatcat ggtcatcgcg agtttaccag gccacagtaa cttaaccttc
     6421 accccgtcat aaaaaccaaa acagtcatcc cagggaccgg cttgaggaga gatacgattt
     6481 ccagttggca gttcatcact acccgcttct tcttgccagt ccgcatcaaa aagcacctgc
     6541 aacgattctg tattttgcgg agtcagcttt ttcgcaaacc acggatgcca gcctgcggag
     6601 gccgggaatg tatcggcata tgaatggact tccagttgca aaaccagcgc atcgttctcc
     6661 agcaaaaaag tctgaatcac ttccccttgc cagggccacg gtgaagccag gggcatacgt
     6721 aaagtcaggg aatctgctgt tttgtcgatg atttcccacg ttgagtaaca agccatacca
     6781 tgcaacgcat gtggtggttt attggcgggt aaagaataac attgcccacc agcattcagc
     6841 gtcgcattcc ccagtcgccc tgcccacggc accattggga aacagccata ctgaaaagct
     6901 ctgcgctgcg gctgccattg gcgtaatact tcagaaccaa atgcctttaa cgaggttatc
     6961 ctggcaccat catgcggata aatctgcagg tgaatattgc ctttttccag cgtcactaac
     7021 tctcctacag gcccaggcca tggcgcgtca aacgcctgtt gtagcgccag gtaatgctcg
     7081 ttttttcgtt cagttttcat gagattgttc cctgatgtag cggtaagaag cggtttcttg
     7141 ttcatcggtc ggctcaatca gccagacgca aacaaaggaa agaactgaga cagcaaataa
     7201 aaacagagcg acataccacg gttttcctcc ccctagcgcc agcaaacttg aggcgataag
     7261 ggggcttagt ccgccaagaa tcgcggaaaa ttgataagca aaagaaaggc cggtatatcg
     7321 tactttggta ccgaacatgc gggtgaaaag tgttggctgc acggcaaaca tcatcgtcgg
     7381 acctaagttg tagccgagga tcatactgca ccagatgatg agcgtacttt tgctatccag
     7441 taataagaag aaaggaaacg cgaacaggac acaaaatcct gctcccgaca aatacagact
     7501 tttttgacct atacgatctg ataaccatcc aaccaatgga caactgaaaa taccgatcgc
     7561 cgaggcaatc aacatacccg tcaggggaat atctcgcgac aatgctaatt ggctggaaat
     7621 atagacaata ccaaaggcgt taataatatt agaggagaca ctttccgcca gcctcgcacc
     7681 cagtgccaat aaaatatttc gtggatgttt tttgaataat tcaatcaacg gaggaacgga
     7741 cttttcttta ttattcgtcg ttttttgctt ttgaaaatcc agcgtttctt cggtatgcag
     7801 gcggataaac agcccaacga tcaacatcac cgcagaaagc caaaacggaa tgcgccatcc
     7861 ccaggagaga aactgctccg aagtaagaaa atggttacag agagcaaaaa taccggttgc
     7921 cagcataata cccacagacg cagccgtttg cgggagtgaa cctaaaaatc cgcgtcggga
     7981 ttcgggcgct gattctatgg tcatcaatgc cgctccgcca tactcgccac caagaccaaa
     8041 gccctgaatt aaacgcagcg ccattaatat gagcggtgcc cagatcccaa tttcctgata
     8101 cgtcggaata aaaccaatca aaaatgtgga gcaaccgaca atagccagtg tccagataag
     8161 tgttattttt cgcccaattt tatcgccaaa atggccaaag acgataccac ctaaaggacg
     8221 ggtcaaaaaa ccaacgccaa aggtagcaaa ggcggctata agacctataa aaggatcgct
     8281 gtctggataa aacagcggtg caaataccag tcctgctgcc gtgccaaaaa taaagaagtc
     8341 ataccattcc attaacgcgc ctgcgaaact gcccataacg acacgtctca tggctttacc
     8401 ggaaagccct tcacgcggtg cgggtaactt ttcagacata attttcctct cctggtaggc
     8461 gagaaatctg ggatacgaac cactaaacgt gggcagtttg aattaatgcg catcggttta
     8521 agccgatgcg cattaattta tttcttcaat tcagccagaa cctctgcatc ctcagcatca
     8581 acatttgcac ccagtggttc ccaattaatc aatacgcagg tcaggaaccc caagaatggc
     8641 acgattgcca accagtaaaa cgcttcagtt ttcaacgccc cccacagcat cggccagaaa
     8701 atgagtcctg caattgcccc tgtgcgcata atagcgcggg caaatccgac cccagtaggg
     8761 cgaatagcgg gaggataaga gagtgtggca atggtcatgc ccagcccacc aggacccgct
     8821 gaatggaaga aaataattga tcccaataac cctaaagaca gccatggatt agtcgttgcc
     8881 agtgcgccta acgacagcaa tgctaaggtc acaacagcaa atccatacat cgactgtcgg
     8941 cgagtaccca gtcgttggag aataaacgag ccgatcatcc cgcctgtcac cccacaaagg
     9001 gcattcacga cggcagaacc cgtcaacgca ccagtaagcc cgccacttat tattcccgcc
     9061 aacgtaagag gaagataaac acctacggcg ttatattgcc acgcctgcat ggttgccacg
     9121 acacagccaa gaatggtgcg tttgcggtaa cgatcattaa ataagatgcc atatccacca
     9181 ctgtatttat tctctgcttt ttccgagaat ttcgcttctg ttgtactcga tgccgcaacg
     9241 cgagcctgaa caccataacg ttttcccaga atgtcgcacg cttcctggta acgcccgacg
     9301 cgtgcggccc acattgcgga ttcaccaata aagaagtaac gcaaaatcat gaagatgact
     9361 gcaaaaatag ctccgatgaa tattccgtaa cgccacaatt gttcttcagc gatacccgac
     9421 agaagcagcg gcaagagcac caaataaaca acagttgtcg aaacatacca ggccatttgc
     9481 cagaggttga ccgacgttcc ggttttcttc gacgttgaac cacgaagctc tgccacggca
     9541 ttggttgcca aagggaagtc gatgcctaaa ccaaagccca tgatgacgcg gcacaccagc
     9601 acccaccaga tactgggcgc aatagctacc gcagcagccc cgatggtgca cagccccatg
     9661 ccaataataa atgctcgctt ttgcccaaag cggtttatta ctcggttcgc taataaaccg
     9721 ccaattaacg cggcgattaa tactgacgca ttaacggttg cggcaaggcc tggagaaatg
     9781 ccaaattgag cggcaatgta tttattacca aaccccacca tcgccgcctg gtaggcatca
     9841 atcaatatgc cgcccaaagc aataaaaata acgccgatac tggtgttgat tgcagagttc
     9901 ttattaacaa tatcaattac gtcctgtggc cgactaattg aataagttgc aggactggtg
     9961 atttgactca tcgcagtgtc ctttttaatt gttgtagggt ggcgctgacg tgacgcagcg
    10021 ccagacagag atttagaaaa tcattccggc gcaaattgca gcatgacctt tatggagtca
    10081 ttttgctgcg cgcgttcgaa tgccgccgct gcatcctcaa tggcaaataa atcggtaacc
    10141 agttcactgg tatcaaaccg cccatccgcc agccattcga taacagcatc gaaatcttca
    10201 gggatgtaca tgccagagtt gagcaagtca cgctcgaaac gttgcatcat cggtaatggt
    10261 atttcacgcg gcccacttgg tacgcccatg cacacgacgg tgcctccggc atatacacgg
    10321 gtacatgcat cgttaagcgt ggcctgcgct gcaacgacat caatcgcccc cgtaaatcgc
    10381 acatctgcgg ccagctcacc tggtgcccaa acttctgctg cgcccagctt taacgccagc
    10441 gcgcgtttgg cagcgtcagg ttcaatcacg gtaatcttac ctgcccccat aatgcgcagt
    10501 gcctgcacga tggataagcc tatcgttccg gcaccaatca ccagtacgct ttccagagaa
    10561 gctttcggca ttcggttaac gcagtgacgg gcacaagccg ccggttcggc aaaggccaac
    10621 acttttagcg gtaaagaggc tggtgcgaca tggcaattac gcgcaggaac aatgtgttgt
    10681 gagcgagcaa agcccggtgc gcgaaaacca gcaacctgcg gtggttcaca aagattaaaa
    10741 cgtcctgctt tgcaggctcg gcattccatg caagccatga tgggatcgac cacaacatga
    10801 tcgcccggct gtacattttt aacgtcgctg ccaacttccg taacgcgcgc tgcaatttca
    10861 tgaccgggga cgacaggtgg tttagcaaac ggatgaccgc ctttcagaac atgcagatcc
    10921 gaaccgcata tcccatagaa cacaggcgca atccgcactt cgtgcggttt aagttcctgg
    10981 tgtggtactt caacatattg agtggtgact ttcccaatat ctgaaaataa aacctgggta
    11041 attttatcca ttgttattct ccagaatcga tatccatagg ggattagcgt tgcgcgatac
    11101 cttcatcgac aatttctgcc aaccgttgta aacgaggaac agagacatcg cgcagcatag
    11161 tttgtggatc ttgtgcacca acaaccgacg cccaaaccgc tcgtccagcc aggaagcctg
    11221 acgcaccacc tttcatggca atacttactg cacggccaaa aatatcggca tcaacgccag
    11281 aagaaagaat tacccacggc atcttcatct gatcgttaag ttgctgacag gcggcaagta
    11341 aggttttttc atcgcctttg ccccctaatg gcatctcggc tttgtaaaga tcagcctctg
    11401 ttccaccaag ctctgcggcg gcggcaacaa tggcgctttc acgatcaaag tcccaaccgc
    11461 gacgaggtgg acgcaccaca ggttcaataa tgctaaccag cccggcacta cggcagcgac
    11521 gaacaaattt atcaaccatt gccagacgtt cctctgcggg ttcatcttca cgccagagta
    11581 caagtaattt cattgcctta gcccccattt cacgggcttt atgcgggtcg acggacatat
    11641 ctatttcaac gctatcaacc gggatgccat tacctgggat aaaaagatcg gcagcgacaa
    11701 tcaggccgca agaattagca acagcgccct gctcgactat ttgctcaaga caaaattgtt
    11761 tatccgccag aacggcagaa gcataaggag aaaggattcg ggtcgcggca actttaaaat
    11821 cggtgagtac actgtcggcg atgggttttg gctgccctgc tgccgcaaac atcaagcgca
    11881 tggcctcacg ctgatcgaca gctaacatgg caaatccacc acctgggcgg gagatatctt
    11941 tcagcgtaaa tggggtgaag tgggatgaca tagaaactcc tgttaataga taaaattaaa
    12001 gaccagcctg ctggcgaacc tgatttagca gggcactcca gtcctgccgc cctttgccgt
    12061 cttgacttgc tgcagcgtaa taactttctg ctgcaatccc catattgagc ggtgcccccg
    12121 tctcccgagc gacgtcaact gcaatacgca gatctttcaa cgccagatcc accatgaagg
    12181 cgggtgaaag atcgcctttc agcactttgc aaggccaggt ggtcgtaaaa tgtcctttcc
    12241 ctgctgccgt accactcatc accttaattg cgacatcaag atttaatccc agtgactcac
    12301 ataacacagc ggcctcggag gacagcgcat taagcgcaat gctcatatag ttgttaatta
    12361 gcttcacgcg gatccccatt cctgggccgc cagcctcgac cagctcatta cccatgcaca
    12421 tgagaatcgg gcgtgcacgt gcaatctgct catcagtgcc acccgctaaa atcagcaatg
    12481 tgccatcaat cgcattcacg gacgttctgc ccacgggggc gtccatcata ctgatgcctt
    12541 tttcttgtaa ctcccgtatt aatgcatcgg tctgtaaggg atgaatagtc gacatatcaa
    12601 tcaataacgc gtcagatgaa acagcctcac agacgccttt ttcccccaac agaacctgtc
    12661 gaacaatgtc accattggga agcatagtga tgataaattc agcgcctgct gccgcttccg
    12721 ctggggtttg tgccgcagtg gccccctgcg taactagcgc atcgacggcc tgtgcattga
    12781 catcaaatac ctgcaatgaa tgcccttttt gcaatagatt gttagccatt gggctaccca
    12841 tctgccccag cccgagaaat gcaatggttg ccatgaatac tctcctgtct gacattgaca
    12901 aaataatgac aattgaaaat tcgttttcgt ctgtttttga gcatatttgt aatttatgtt
    12961 caaaaacacg acacgcatca ctaatttgat catttgtgtc atttaaattg accgaagacg
    13021 acaaaattaa acaggacaag acaaatatga ccaaaattgc atgcgtgggt atcacagttc
    13081 aggacaggat ttattctttg ctcactctgc cggatggcgg cggaaaatat caggcaaatc
    13141 attatcttga gattggtggc ggcccggcag caacagccgc tgttgcaatc gcaaagcttg
    13201 gcgtagaggt tgatttcatc ggtcgcgtcg gcgatgacag ttgtggaaat accttacttg
    13261 cagaacttga gggttggggc gtcaataccg cattttgccg ccgatatcct aacgcccgtt
    13321 catcgcaatc agcgatcctc gttgatcagc atggcgaacg gattatcgtt aattatccaa
    13381 gtcccgatct cggtactgat gccgaatggt tagaagcgat cgatttctct cgttatgatc
    13441 tgattctggc cgatgttcgc tggcatagcg gtactgaaaa agcattctcg ctagcgcgtc
    13501 ttgcgggtgt gacaacgctt ttagacgccg atatgacacc gcaggatatt tctccacttg
    13561 tcgcgcttgc cgatcacgcc gtgttctcaa caccagggct gaaacgcatg actggcctgc
    13621 agtcacctga agaaggtttg ttccaggcaa cgacataaac agcaggtaaa gtgtatgtta
    13681 cgctgggttc tgaaggatcg ttgtggattg aagatggtca tttgtgtcaa caggaggcat
    13741 tttctgtcaa tgtggttgat accactggag caggcgatgt ttttcacggc gcactcgcgg
    13801 tagcgttggc agaaaaaatg ccaacaaaag aggctattcg ttttgccagt gcggttgccg
    13861 cgatgaaatg tacccagcct ggcggtcggg ccggcattcc taatcgtgag caaaccgaat
    13921 catttttgtc actttatgcg taaaatggta aacatctacc gaggaggggc aagggagtat
    13981 tcatgagtat catcgaagtg acaggtaatc cacgccatga ccaactcgtt catctaatcg
    14041 cagaacgcgg ttatatgaat attgaagaac tggcacaact gctggatgtt tcaacgcaaa
    14101 ccgtgcgccg ggatatccga aaactcagtg agcagggctt gatcacccgt catcatggag
    14161 gggccgggcg cgtctccagc gtcatgaata ccgcttttga gcaacgggaa ctttcgctca
    14221 ccgccgagaa acgggcgatc gctgaggcag tcgccgatta ccttcccgaa cgctgtaccg
    14281 tctttatcac catcggtaca accgtagaag ccgttgccag ggcattactc aaccggcgtg
    14341 atttacgcat tatcactaac agcctacgtg tggcacagat tctttataag aatcaggata
    14401 ttgaagtgat ggtgccggga ggcactttac gcgctcataa cggcgggatt atcggcccag
    14461 gagccgtgga ttttattgaa ggcttccgcg cagattattt aatcaccagt ataggtgcca
    14521 ttgaacatga cggcacccta ctggaatttg atgtaaatga agcgttagtc gcgagaacga
    14581 tgattaaaca tgcgcggaat acattgttag tggccgatca tacgaagttt gccgcgtctg
    14641 ctgccgtttc aattggcaat gcacggaatg tcagggcttt ttttacagat gccccgcctc
    14701 ccaattcttt ctgccagttg ttaagtgaag agaatgttga actggtggtt gccgagcagg
    14761 aagtttcctg aaattgaaac ttcgaaaaat ccgctgggga ttcagcggat tttattttgc
    14821 cggatgcggc ctacggttga tacggttttc gttggccgga ttaagcacat ccggccatta
    14881 attaagcttc agccgccagc atctctttct tacgttggct aatacgctta accagcatta
    14941 cggaaaccac cgcaccacag aatgcgaagc aggccataat gccaaacacg attttgtagc
    15001 caataatccc cggatttaaa tccagaatgt agccatacag actgaagcag aacatcgccg
    15061 gagcgtaacc aataaagcta cccaacgcca tcgccgcgcc tgttttattt tcagcaattt
    15121 ttgcttcgcc gataggtgca aaaaatacgg ctcgctgtgt aaagactatc gcgccaaagc
    15181 ccagcgtaca tgccatccct aaatagaccg gcatactttc gtgcggcagc ataatcaaca
    15241 gtacgagcgc agcggtactg atgataaagg tgtagcatag atatttactc ggcgatttca
    15301 ggatcttatc tgaaatcatg ccaccaatcg gtccaccaat cattttcaga cagtattggt
    15361 taatgatgcc gtaagccccc accagcgcaa cgggcaatag atagatgttt ttcaggaatg
    15421 gaatgaagaa tgtcaggcca cagtaaaccg cgtaaacgaa gaagacgtta aaagcgataa
    15481 gccagatggt tttgtctctc agcaccgagg tcatactggt gttttgcgat gctccgtctt
    15541 cttttttcac ctcaacggac ggtgcctctt ctttgtcatt caggacaaag aaaataataa
    15601 tgcctacggc aatcacaata agggaataga accagatgcc tgctttaaaa cccaataagc
    15661 cactgccaaa ccaggtaaat actgccaacg cagaaaatgc caccacggta tcgacaatgc
    15721 cacgccctgt ttcgaagaag ccaaacaacc gaccttgttg ttcgctattg cccaatcgac
    15781 ttaccgactt gagcaagacc ggccagttca tcatgtcgca agtaacgcca aatagcgccc
    15841 agacaaagag gatgccccaa taccccggca ttgtcgtcaa ataaacaccc agtaatcctg
    15901 tcgcaatgag tgagaatgac atggtgtatc tgcgcggtag tttatcggca aaataaatag
    15961 acagaaaaaa acccactgtc gtgacaaatg agtttaccga catagcatta ccaatttgac
    16021 cattggtcaa atggaaatat tcctgcatcg ggatataaaa cgcatctttc agcgacggta
    16081 atttataaat tgtaccgcca cacaatgtta atagactaaa taacgcccat ttctttttcg
    16141 tgagcataac atcccctaag cgatttttaa aatcactgtt ttaaaggcaa taagataccg
    16201 accattcata tggaatggtt ttattaactg cgtatgagtt aaaacttatt agtggttcag
    16261 aagatggttt gcctcttgtt tgatttgttg tagtacgtta cgcacatgat ttatctgatt
    16321 aattgcatct tctttttctt tacgtaaccg cgcatccagt aaatgatttt ctggcaatgg
    16381 tgtttcactc atctggcgat aagggatcca cggattatca tcttcgtctt caaagcggaa
    16441 cgccggcgca tagtaatcaa cctcttcttc caggccatat gccgtcacct gcggctcatc
    16501 atcacccagg catataaggt gcgttaataa cgctttgaac ggcatatcac cctgacctga
    16561 aatacaggct ttatgaccca ggccacccgg ttctttaacg atcaaggcat ctttgatatg
    16621 gacctgggta atatgcggcg ccatcgtttt taaagcgtcg atgggatgct catttgcatt
    16681 gatcatgttc gcaaaatcaa acagtaagga aagagattcc atctcacttt ctttgaccag
    16741 cgacaccagc tcatggcttt ttaaatcttc atgctgttcg atcgtaaaag tcaggccgct
    16801 gtcctgatac gtttcccgta cataggcaat gtcgttagcg atgatcgata atacgtcgcg
    16861 caaattacct tcataacgtg gataaaaacg tacggacgat gccccagttt tcaacgcaat
    16921 ggcgacggct tcgtcaatag atgccttatc tgaggcgctg gtttcaatat gaatatcaag
    16981 gttcagacgg cgggctttat ctccaaaggc agagagttct ttatcgtcca tattaccaag
    17041 agaaaaacgc tcgccatcca gaacatggat tttgacgcca cgtagattat tttcacttgc
    17101 gatatcaagt aaatcagcag gcaacactct ttccagccgc atatttaaat gaaaggcata
    17161 agcatgtaaa taaagaggaa gagtgtcgac acgttgtaga atctttcttg cgttatttat
    17221 tgtcaccata aaacacctac agattaatga tgaggtctac ggcctcaaat cgccaatatt
    17281 caatatctat ctggacaata ttgccttgct ggtcggcgcg atgcttttca attaagatcg
    17341 ctggcatacc cgcagaaccg ccaagatact tgctgatatc gcccggcatt ctgacgggtt
    17401 tgaaaatcaa tttttttact accgtctctt tttggtagca cttttcccag actgcagaaa
    17461 atgagtggtt ttcaagttgt tcaataaaac ccggagcaac ttcaggatta atatatgttt
    17521 cgtgatagaa aactttatgt ccttccagcg ccccccagcc ggtgatgcga tagagttcag
    17581 ttgatggtgt cactgcaatc agtggcgcga gcgtctcggg aatatcgctg gtacggtttt
    17641 tctcggtaaa cccccaggaa ggctctcgtc cttgctcaat tgcggcccgc tgaaagctcg
    17701 ccgacaactc cggactgtaa ttaaatcgcg gctgggtcac gaaccacccc ttacgatcct
    17761 tacggaagat tttggattct gcctcgaggt tcaacaacgc ctggcgcagc gtcatacgtt
    17821 taatgcccag cagttctccc agctctcttt ctgaagggag ttttccaccc ggcgtggtga
    17881 tgccatcttt caaccaccga tccagtttct cttttgcatt ttctacggtg gattgattct
    17941 ccgccattgt ttaatccttt tggtttaaac cagatgaagc atcttacgct tacgccaaaa
    18001 aagaaagcaa ttaacggcgt atgactttgc agataattgt gacaaagctc tctattgacg
    18061 taatcccaga cttataaggg agatactcgc attggtgtgc gctcacgaca ggtaaaaaaa
    18121 aacctgccag cgatggcagg ttttttataa ctaaaaaacg aaattaatcg tctttcggtg
    18181 cgcggttggc gcggcgacga tcgttttccg tcaggtgacg tttacgaata cggatagagg
    18241 tcggagtcac ttctaccagt tcgtcatcat cgatgaactc cagagcttgt tccagagtca
    18301 tgcggatagg cggaaccaga acaacggctt cgtcagtacc ggaagcacgc atgttggtca
    18361 gtttcttacc ggtcaggcag tttaccgtca ggtcgttaga gcggctatga ataccgataa
    18421 tctgaccttc gtaaacttct gcaccgtgac cgaggaacag cttaccgcga tcctgcagac
    18481 cgaacagcgc gaacgcgacc gctttaccct gaccgttaga gatcagtacg ccgttctgac
    18541 gctgacccac ttcacccgga cgtacgtcgt cgtagtggct gaaggtggag tacagcagac
    18601 cagtaccaga agtcatggtc atgaactcag aacggaagcc aatcagacca cggcttggga
    18661 tcacgtagtc gagacgtacg cggcctttac cgtctggatt catgtttttc aggtcgcctt
    18721 tacgttcgcc cagcgcctgc attacagaac cctgatgctg ttcttcaacg tccagagtca
    18781 cgttttcata cggctcttgt ttacgaccgt cgatttcacg gaagataact ttcggacggg
    18841 ataccgccag ttcgaaacct tcacgacgca tgttttcgat cagaacagac aggtgcagtt
    18901 cgccacgacc agaaacgcgg aacgcatcgg cgtcttcggt ttcttctacg cgcagcgcaa
    18961 cgttgtgtac cagttctttg ttcagacgat ccaggatctg acgagacgtt acgaacttac
    19021 cttctttacc gcagaacggc gaggtgttaa cgcagaagaa catagaaacg gtcggctcat
    19081 caacggagag tgccggcagc gcttcaacgt tttgcgtgtc gcaaacggtg tcagaaatgt
    19141 tcagttcgcc aaggcccgtg atcgcaacga tatcgccagc ttccgccaga tcggtttcga
    19201 tacgttccag accgaggtgg cccagcactt taccgacttt cgcgttgcgg gttttgcctt
    19261 cgctatcgat gatagtgacc tgctggttcg gcttcacttt accgcgcttg atgcggccaa
    19321 tgccgataac gccaacatag ctgttgtaat cgagctgaga aatctgcatc tggaacggac
    19381 cgtcaaggtc aacgtccggc gcaggaacgt ggtcaacaat cgcctggtac agcggggtca
    19441 tgtcttccgc catatcttcg tggtccagac ccgcgatacc gttcagcgca gaagcgtaaa
    19501 cgatcgggaa gtccagctgc tcgtcggtcg cgtcgaggtt aacgaacaga tcgaatacct
    19561 gatccacaac ccaatcagga cgcgcgccag ggcggtcaac tttgttgata acaacaatcg
    19621 gcttcaggcc gtaagcaaac gcttttttgg ttacgaagcg cgtttgcggc atcgggccgt
    19681 caaatgcgtc aaccaccagc agcactgagt ctaccatgga cattacacgt tcaacttcac
    19741 caccgaagtc ggcgtgcccc ggggtatcaa cgatgttgat acggtaatca ttccatttga
    19801 tagcggtgtt tttcgcgagg atggtaatcc cacgctcttt ctccaaatcg ttggagtcca
    19861 tcacgcgctc ttgggtttcg gcacgagagt cgaacgtacc ggattgttgg agcagcttgt
    19921 ctaccagggt ggttttacca tggtctacgt gcgcgatgat ggcgatatta cgcaattttt
    19981 cgatcacaac tttgcctcag gcattagaaa tagcgcgtta ttgtacacgg attaatcgca
    20041 ctacaaaaca ggatcacaaa catcctccgc aaacaagtat tgcagagtcc ctttgtgatc
    20101 gctttcacgg agcataaaaa gggttatcca aaggtcattg caccaacatg gtgcttaatg
    20161 tttccattga agcactatat tggtgcaaca ttcacatcgt ggtgcagccc ttttgcacga
    20221 tggtgcgcat gataacgcct tttaggggca atttaaaagt tggcacagat ttcgctttat
    20281 cttttttacg gcgacacggc caaaataatt gcagatttcg ttaccacgac gaccatgacc
    20341 aatccaggag agttaaagta tgtccgctga acacgtactg acgatgctga acgagcacga
    20401 agtgaagttt gttgatttgc gcttcaccga tactaaaggt aaagaacagc acgtcactat
    20461 ccctgctcat caggtgaatg ctgaattctt cgaagaaggc aaaatgtttg acggctcctc
    20521 gattggcggc tggaaaggca ttaacgagtc cgacatggtg ctgatgccag acgcatccac
    20581 cgcagtgatt gacccgttct tcgccgactc caccctgatt atccgttgcg acatccttga
    20641 acctggcacc ctgcaaggct atgaccgtga cccgcgctcc attgcgaagc gcgccgaaga
    20701 ttacctgcgt tccactggca ttgccgacac cgtactgttc gggccagaac ctgaattctt
    20761 cctgttcgat gacatccgtt tcggatcatc tatctccggt tcccacgttg ctatcgacga
    20821 tatcgaaggc gcatggaact cctccaccca atacgaaggt ggtaacaaag gtcaccgtcc
    20881 ggcagtgaaa ggcggttact tcccggttcc accggtagac tcggctcagg atattcgttc
    20941 tgaaatgtgt ctggtgatgg aacagatggg tctggtggtt gaagcccatc accacgaagt
    21001 agcgactgct ggtcagaacg aagtggctac ccgcttcaat accatgacca aaaaagctga
    21061 cgaaattcag atctacaaat atgttgtgca caacgtagcg caccgcttcg gtaaaaccgc
    21121 gacctttatg ccaaaaccga tgttcggtga taacggctcc ggtatgcact gccacatgtc
    21181 tctgtctaaa aacggcgtta acctgttcgc aggcgacaaa tacgcaggtc tgtctgagca
    21241 ggcgctgtac tacattggcg gcgtaatcaa acacgctaaa gcgattaacg ccctggcaaa
    21301 cccgaccacc aactcttata agcgtctggt cccgggctat gaagcaccgg taatgctggc
    21361 ttactctgcg cgtaaccgtt ctgcgtctat ccgtattccg gtggtttctt ctccgaaagc
    21421 acgtcgtatc gaagtacgtt tcccggatcc ggcagctaac ccgtacctgt gctttgctgc
    21481 cctgctgatg gccggtcttg atggtatcaa gaacaagatc catccgggcg aagccatgga
    21541 caaaaacctg tatgacctgc cgccagaaga agcgaaagag atcccacagg ttgcaggctc
    21601 tctggaagaa gcactgaacg aactggatct ggaccgcgag ttcctgaaag ccggtggcgt
    21661 gttcactgac gaagcaattg atgcgtacat cgctctgcgt cgcgaagaag atgaccgcgt
    21721 gcgtatgact ccgcatccgg tagagtttga gctgtactac agcgtctaat agttgaagtt
    21781 gtactacccg gcgcaacaac gccggaggtt caaaccaggc ccatctggcg taattgttgc
    21841 agccagtttg aacacggaca gcgcgcagaa cccggagcgt acactggtac gtgaggagtt
    21901 cgagcactgc ccaggttcaa aatggcaaat aaaatagcct gatgggactg gtttttagtt
    21961 gccgtggaaa tttcagccca tctctgcatg ggcttttttc tccgtcaatt ctctgatgct
    22021 tcgcgctttt tatccgtaaa aagctataat gcactaaaat ggtgcaacct gttcaggaga
    22081 ctgctttatg gcaacaggca cgcagcccga tgctgggcag atcctcaact cgctgattaa
    22141 cagtattttg ttaatcgatg acaacctggc gatccattac gccaaccctg ccgcgcaaca
    22201 actgctcgcc caaagctccc gcaaattgtt tggtacaccg ttaccggaac tgttgagcta
    22261 cttctcatta aatatcgagc tgatgcaaga aagtctggag gcggggcaag gttttaccga
    22321 taacgaagtg acgctggtca tcgacgggcg ctcgcatatc ctttctgtga cggcccagcg
    22381 tatgccggac ggcatgatcc tgctggagat ggctccgatg gataaccagc gccgcttaag
    22441 tcaggaacag ctacagcacg cccagcaggt tgctgcccgt gatttagtgc gcggcctggc
    22501 acatgagatt aaaaatccgc ttggcggttt acgtggcgcg gcgcagctgc tcagcaaagc
    22561 gttacctgac ccatcactac tcgaatatac caaagtgatt atcgaacagg cggaccggct
    22621 gcgaaatctg gtcgaccgtc tgttggggcc gcagctgccc ggtacgcgcg ttaccgaaag
    22681 tattcacaaa gtggctgaac gcgtggtaac gctggtgtcg atggaactgc cggacaacgt
    22741 gcggttgatt cgtgattacg atcccagcct gccggaactg gcgcacgacc cggatcaaat
    22801 tgaacaggtc ttgctgaata ttgtgcgcaa tgcgctacag gcgctggggc cggaaggcgg
    22861 tgaaatcatt ctgcgtaccc gcaccgcgtt tcaactgacc ttacacggcg agcgctaccg
    22921 gctggcggcg cggattgatg tggaagataa cgggccgggc attccgcctc atttgcagga
    22981 tacgctgttt tacccgatgg tcagcggccg cgaaggtggc accgggcttg gcttatccat
    23041 cgctcgtaat ttgattgatc agcattcagg caaaattgaa tttaccagtt ggccagggca
    23101 taccgagttc tcggtttacc tgcctatcag gaaataaagg tgacgtttat gcaacgaggg
    23161 atagtctggg tagtcgatga cgatagttcc atccgttggg tgcttgaacg tgcgctcgct
    23221 ggggcaggtt taacctgtac gacgtttgag aacggcgcag aagtgctgga ggcgctggcg
    23281 agcaaaacgc cggatgtgct gctttcagat atccgtatgc cgggaatgga cgggctggcg
    23341 ctgctcaagc agattaaaca gcgccatcca atgcttccgg tcatcattat gaccgcacat
    23401 tccgatctgg atgctgccgt cagcgcctat caacaagggg cgtttgatta tctgcccaaa
    23461 ccgtttgata tcgacgaagc agtggcgctg gttgagcgcg ctatcagtca ttaccaggaa
    23521 cagcagcagc cgcgtaatgt tcagcttaac ggcccaacga ccgatatcat cggcgaagcg
    23581 ccagccatgc aggacgtgtt ccgtattatc ggtcggcttt cgcgttcttc tattagcgtg
    23641 ctgattaacg gcgaatccgg caccggtaaa gaactggtcg ctcatgccct gcatcgccac
    23701 agtccgcgcg ccaaagcgcc gtttatcgcg ctgaatatgg cagctatccc aaaagatttg
    23761 atcgaatcag aactgtttgg ccacgagaaa ggcgcgttta ctggcgcgaa taccattcgt
    23821 caggggcgtt ttgaacaggc cgatggcggt acattattcc tcgacgaaat tggtgatatg
    23881 ccgctggatg tgcagacgcg tttgctgcgc gtgctggcag acggtcagtt ttaccgcgtt
    23941 ggcggctatg cgccggtgaa agtggatgtg cggattatcg ctgccactca ccagaatctc
    24001 gaacagcgag tgcaggaagg taagttccgt gaggatctgt tccaccgcct gaacgttatc
    24061 cgcgttcatc tgccgccgct gcgcgaacgt cgggaagata ttccccgtct ggcgcgccat
    24121 tttttacagg ttgccgcgcg cgaactgggc gtagaagcga agttactgca tccggaaacc
    24181 gaagctgctc tgacgcgtct ggcgtggcta ggcaacgtgc gccagctgga aaacacctgc
    24241 cgctggctaa cggtgatggc cgccgggcag gaagtgttga ttcaggattt gcccggcgaa
    24301 ctgtttgaat caacggttgc ggagagtact tcgcaaatgc aaccggacag ctgggcgacg
    24361 cttcttgcgc agtgggcaga cagagcgctg cgttccggtc atcaaaatct gctttccgaa
    24421 gcgcagccag agctggagcg gacgttactg acgaccgcgt tgcgacatac gcaggggcat
    24481 aaacaggaag cggcgcggct actcggctgg ggccgcaaca ccctgacgcg taagttaaaa
    24541 gagctgggga tggagtgatt cacagcttgt gtgtaaagat tgattattga gcgcaaattg
    24601 ctggtatttt acgctttact gttccgataa gttcagtatg atcttgcccg gaaaacgggg
    24661 agagtcatta tgctggaatc aataattaat ctggtatcga gtggcgcagt tgacagccac
    24721 acaccgcaaa ctgccgttgc tgccgtgctg tgtgccgcaa tgattgggct gtttagttga
    24781 gtttcagatt gctgatgaag tgctctttgt tcatgccgga tgcggcgtga acgccttatc
    24841 cggcctacaa aagcaatatt ttcaacaggt tttgtaggcc tgataagcgt agcgcatcag
    24901 gcaatcttgt tctcagcaca ttgatggcgc tttcgttttc tactttgtaa acgaagcgcc
    24961 attcactatt taaatcaccc gtgagaactg ctgcatccgc gctttctggc gcagataggt
    25021 atcaaagcac atgcaaatgt tgcggatcag caagcgacct ttcgccgtca cctgtattcc
    25081 cttctcatcc acatccacca gcccatcttt tgctaacggg gcgagcagct tgagatcttc
    25141 cgcaaagtaa tcagcgaagt gcaaatccca ctgtttctca ataggggcgt aatccagacg
    25201 gaagttgcag atgagcgact taatcacatc gcggcgaata cagtcatcac gcgttagcgc
    25261 aataccacgc cacagcgcat tgccttgttc atccacttgc tgatagtact gcttcaactc
    25321 tttctggttc tgcgcgtagc agtcgccaat catgctgatg gcggaaacgc ccatccccag
    25381 cagatcggta tcgccctgag tggtgtagcc ctggaagtta cgatgcagca cgccttcacg
    25441 ctgggccacc gccagctcgt catccggacg ggcaaagtga tccataccga taaactgata
    25501 gcccgattgc gtcaggaagg cgatggtttc ctgcaggata tcgagttttt gctgcggact
    25561 cggcaggtca gcatctttga ttttgcgctg agcagcaaaa atggtcggca gatgcgcgta
    25621 gttaaagaca ctcagacgat cggggttcag ctccgccaca cgtttcaggg taaaggcgaa
    25681 actctccggc gtctgtttcg gcaggccgta aatcaggtcg atgttggtgg aggtaaaacc
    25741 aatctcacgc gcatggttaa gcagtgcaaa gatgaactct tcatcctgct cgcggttaac
    25801 cagacgttgc acttctttgt tgaagtcctg cacgcccatg ctcaggcgat taaagccttc
    25861 ggcgcgtaaa tgatcgagta catccagttc gatttcccgc ggatcgactt cgatcgaaat
    25921 ctccgcatcg gcattgaact ggaagttttc gcgcagcagc ttcatcaggc ggctgatttg
    25981 cgctttattc agatacgtcg gcgttccgcc gccccagtgc aattggctga cgtgacgccc
    26041 ggcgaacagc ggtgcacaat ggacgatttc ttgctccagc gcgtccagat actgatcggc
    26101 cttgtgctgc tggcgagtaa caatcttatt gcaaccgcag aagtaacaaa gcttatggca
    26161 gaacgggata tgtacgtaga gagataatgg acgctcagga tagcgcgcca cggcttgtaa
    26221 aaacgcctgt tcgccgaagt cttctgaaaa ctccagcgcg gtcgggtacg aggtgtatcg
    26281 tggcccggaa tagttatatt tctggatcag ggccagatcc cagtcgattt gctgtacaga
    26341 catgctcact ccttccgatg gcgtctctgg cggctacggc gtaccggcgg atgcccgttt
    26401 cgggtcatca gccggttgcg cagccacttt tgtcgccgcg acaaccgctg tagtttaacg
    26461 aataaccaca ccagataaca tataaccgga agggttatca gcagaacggg aaggcccacg
    26521 gcggcaatcc gttagttgcc ccgcagcaga cgcatcatgt cttcttgctt ctcgtcttct
    26581 tcctcttctt cgtcatcgtc ataagagagg ccgagtttct gcatcaactc atcaatacga
    26641 tccagtttag catccaccca ggattgctct tcggcgctca gggtttcgcc tgcttccaga
    26701 cgttccagca gcgcatccag acgctcatcc gtttccagta attccaactc cgcctgcggt
    26761 gaaagcatag gtttctcact cttcggtttg tgctgtttgg tgactttttc agtcacgccc
    26821 aatggaatag gggttttact gccaatacgt ggatcttttg gtgcgttctg gcctttgctg
    26881 cctgacgtgg tgtttccgcc cgctgcgcgg ctgcccggcg catgaccacg acgttttttc
    26941 tgacgcttgc gatcacgagc ttcctgatcc agctcctcgc gtgtttttcg acgcgctttt
    27001 gcatgacctt tgctgcgtga gtttgaagat gatggtttca taatggttcg tcttttagcc
    27061 gttttattca gtatagattt gcggcggaat ctaacagaaa gcaagcaaag aaaaaaggcg
    27121 acagattact ctgtcgcctt ttttcctgac tcataaccct taacgggtct tacaatcctt
    27181 gcaggccgcc aacacaccct gtttttcctt tagccggaac aatccgtgtt gattccattt
    27241 ccgtttaatt acgtctccgg acgtttgtct tcctgacaat cctctgtctt cgcctcctgg
    27301 cgctcctgac cctcatcctg agtcattgtt cctgttagcg tcctcgctct atgggtgcta
    27361 ctttacgcct ttgctttaaa caaacaatca accgccatca gtatttgcac attttcagag
    27421 ttttatataa aagcaattca ctgatttata tagcttattt gacatgcatc gcagaatttt
    27481 ctctgctatt tcgcataaag actatcggca atgtctcaca aagcgtatgg cttttactta
    27541 caacgctcac gccgaaaaat ccgcctttta ttatggttca ggtataatcg ccacaaagct
    27601 ttgactgacg gagacgaccg ctttgactaa tttgaattat caacagacgc attttgtgat
    27661 gagtgcgcct gatattcgcc acctaccttc cgataccgga attgaagtgg cttttgcagg
    27721 ccgttccaac gcaggtaaat ccagcgcgct gaacacgctg actaaccaga aaagcctggc
    27781 tcgtacctca aaaaccccag ggcgcaccca gcttatcaac ctgtttgaag tggctgacgg
    27841 caagcgtctg gttgacttgc ctgggtacgg ttatgcggaa gtcccggaag agatgaagcg
    27901 caaatggcag cgtgcgctcg gcgaatacct cgaaaaacgt cagagcctgc aaggtctggt
    27961 ggtgctaatg gatattcgcc atccgctgaa agatttggat cagcagatga ttgagtgggc
    28021 ggtagacagc aatatcgccg ttctggtgct gctgaccaaa gcggacaaac tggcaagcgg
    28081 cgcacgtaaa gcgcaattga atatggtgcg tgaagctgta ctggcgttta acggtgatgt
    28141 gcaggttgaa acgttttctt cgttgaagaa acaaggcgtg gacaagctgc ggcagaaact
    28201 ggatacctgg tttagcgaga tgcagcctgt agaagaaacg caggacggcg aataattttc
    28261 ttgccttaat gcttgtgccg gatgtggcgt atccggcccg taaattcaat agattacaat
    28321 tctaccgttg gcctgataaa catagcgcat caggcattac ggatcttttc tttcgcccaa
    28381 taaaaaacgc cccagtcatt actgactggg gcggctaaaa tattcagcca aatccgatta
    28441 cgtgaagtaa aaggtctgaa agatagaaca tcttacctct gtaccctacg cgactaactt
    28501 tactcttttt tgttcagttc agaaagcact ttttgtaatt ttttttcaat tgttgcatag
    28561 gaatttctaa tagccatcac aaaacgtgac agcttatgtt gcttacttac gaaaaaaggg
    28621 catgttcagg cgaatcttag tgcgcctgat cccagttttc gccactcccc acttccacca
    28681 gcaacggcac atccagacgg gtacagtttt ccatcagttg atgaatctgc ttcgcgacgg
    28741 catcaacatc atctttatga acttcaaata ccagttcatc gtgtacctgc atgatcatac
    28801 gtacacgcgg ttgctcagcc tgtaaccacg catcaacggc aatcatcgcc cgtttgataa
    28861 tgtcggcggc ggttccctgc attggcgcgt taatggctgc acgttcagcc gctgcacgac
    28921 gagcaccatt gctggatttg atatccggca gatacagacg gcgtccgtcc agcgtttcaa
    28981 cgtagccctg ctctttcgcc tgagcacggg tgcgttccat atactccagc acgccagggt
    29041 agcgttcgaa gtaaaggtcc atgtacttct gcgcttcttt acgtggaatg ttcaattgcc
    29101 gcgccagacc gaaagcactc atgccataaa tcagaccaaa gttgatcgct ttcgcgctac
    29161 ggcgttgctc gctggtgacg gtttccagtg gcaaaccaaa cacttctgcc gccgttgccc
    29221 ggtggatatc ttttccttcc gcgaatgcgg tcagcaagcc tttgtcacgc gagagatgcg
    29281 ccataatgcg cagttcaatc tgcgagtagt ccgctgagac aatcacataa tcctctggcg
    29341 caataaacgc ctggcggata cgacgacctt cttcattacg caccggaatg ttttgcaggt
    29401 taggatcggt tgacgataaa cgtcccgttg cagttactgc ctggtgataa gaggtatgca
    29461 cacgcccggt tttcgggttg atcatcagcg gcagcttgtc ggtgtaagtc gactttagct
    29521 tcgccagacc acgatactcc agaatcactt ttggcaacgg atagtccagc gccagttctt
    29581 ccagtacctc ttccgacgtt gacggcgcgc cacccggcgt tttcttcagc ggtttaatgc
    29641 cctgtttttc aaacagaatg gtttgtaact gcttggtgga agaaaggtta aattcctcac
    29701 ctgcaatttc atgcgctttc ttttccagct cagccagacg aagggtgagc tcttcagaat
    29761 gattgtgcag cactttcgga tcgatcttca caccgttacg ttcaatgcgt gaaagcaccg
    29821 gcaccagcgg catttcgata ttctcgaaga cgttcaacgg ccctttgtgt ttttgcagat
    29881 ctggccacat tttcagatgc aactgcaagg tgacatctgc atcttcggcg gcgtaacgtc
    29941 cggcttcttc gagggcaatc tggttaaagg tcagttgatt tttgccttta cccgcaatct
    30001 cttcaaaagt gatggttttg tgcttcaacc aacgttccgc gaggctgtcc atatcgtgac
    30061 gtccggcaac gctattgaga atgtaggact ccagcatggt atcaaacgca atcccacgca
    30121 gctcaatgcc gtaattcgcc agaataccgc gatcgtattt caggttttgc ccgaccttca
    30181 gcgccttttc atcttccagc agcggtttta gcaactcgag tgcacgctcg cgagagattt
    30241 gatcgggcgc gtcaagataa tcatgagcaa ccggaatata tgccgctacg cctggctcga
    30301 tagcaaaaga aagcccgacc aggttagcag agatgttatc aaggctgtcg gtttcggtat
    30361 caaatgcaaa taccggcgct ttttccagct tcgcaatcca cgctttcagt gtttcttcat
    30421 caaggatggt gacgtagttg tcataagaaa tcaccgttgc cgtcacttct ggtgcttcgt
    30481 ctgcaacact ggtttcctgt ggcttcgcgg ctggttttgc ccctttggcc tgtaaccatt
    30541 tgcccgcttc gacatcagca gtccagcgtt tgaactcata ctttttgaac agccccaaca
    30601 actcttccgc tgccggttgc tgcacttcca gttgttcaca ggtcagctcc agttcaacgt
    30661 cggttttaat cgtcgccagc tggtatgaga gataagcaac ttctttgttt tgctcgagct
    30721 tcgctgccat tgttttcgcg ccacggaagc tcaacccagc aattttttct ggctcggcat
    30781 acagcgtatc cagtccgcca agaccttgca gcaatgcctg cgcggttttt tcaccgacgc
    30841 ccggtacgcc aggaatgtta tcagaggagt cacccatcag cgccaggaaa tcgatgatca
    30901 gttctggcgg cacgccgtac ttattcacca cctcttccgg tccgaggatg gtattcgtca
    30961 tggtattgat aagcgtaata tttggcgtca ccagctgcgc catatcttta tcgccagtgc
    31021 tgatcagcac cggacgcccg gctttttcgg cttcgcgcgc cagagtaccg ataacgtcgt
    31081 ccgcttctac gccagaaacc gccagcagcg gcagtcccat cgctttaacc atcgcgtgca
    31141 agggttcgat ttgtgcacgc agatcgtccg gcattggcgg gcgatgtgat ttgtaatgtt
    31201 caaacagttc atcacgaaag gtttttccct tggcgtcaaa gaccaccgct gcatgcgtcg
    31261 gtttatattg catgatcaga ctgcgcagca tgttgaggac accatacatc gcaccggtcg
    31321 gctcgcctgc gctgttagtc agcgggggaa acgcgtgata tgcgcgataa agataagatg
    31381 aaccatctac aaggataagt ggattttggg ggatctgaac cataatgtcc gtgcctgttt
    31441 atcagattat gggtaaagga tgccacagac aggatgaaaa catgagtttt tcaccgcttt
    31501 tcgtgaaaat cttttgtaga tcttctggat cgctcgcaaa tcgtcatgtg gataactttg
    31561 tgcataaatt atttacatcg cttttaattt attatcatgc tttttacagc atgatgtttg
    31621 tctttgtttt tcatgcagtt aaaaataaat ccatccctaa taattgagat aatgggattt
    31681 tttctaatgt ggatagatat gaattatttt tctccttaag gatcatccgt tatttgggtc
    31741 gttttctgct aaaatctgcg ccttgcgctt ctttagcgca ctgtctatgg ctaacctttt
    31801 gaataaattc attatgacga gaatactcgc tgcgataacc cttttgctga gtatcgtatt
    31861 gaccattttg gtcactattt tttgttccgt cccgatcatc attgccggga ttgtaaaact
    31921 attgctacct gtgccagtca tttggcgaaa ggtttcgcgt ttttgtgatt ttatgatgta
    31981 ttgctggtgt gaaggtctgg cggtattact gcatctgaac ccacacctgc aatgggaagt
    32041 tcacgggctg gaagggttaa gtaagaagaa ctggtatctg cttatttgta accaccgtag
    32101 ttgggcagat attgtcgtac tgtgcgtgct gtttcgtaag cacattccga tgaataaata
    32161 tttcctcaag caacagctgg cctgggtgcc atttcttggc ctggcgtgct ggtcgctgga
    32221 tatgccgttt atgaagcgtt attcccgcgc ttatttatta cgtcatccgg agcgacgtgg
    32281 taaagatgtt gaaaccactc ggcgttcttg tgaaaagttt cgcctgcatc ccaccaccat
    32341 tgtcaatttc gttgaaggct cccgtttcac gcaagaaaaa catcagcaaa cccactccac
    32401 ttttcaaaac ttgttgccac caaaggcggc aggcattgcg atggcgctaa atgtactggg
    32461 taaacaattc gataaactgc ttaatgttac gctgtgttat ccggacaata accgtcagcc
    32521 gttcttcgat atgttaagcg gtaaactgac gcggattgtc gtacatgtgg atttacagcc
    32581 tattgccgat gagttacatg gcgactacat caatgacaaa agctttaagc gccattttca
    32641 gcaatggctg aactcattgt ggcaggaaaa agaccggctt ctgacgtctc tcatgtcatc
    32701 acagcgtcag aataagtaag ttttactcgc ctctgaatga gcagaggcga gtgagtattt
    32761 taatgaacaa aacgtccggc acgagacata aattcttctt cgctcatctc ctgtccgtta
    32821 aaaacaactt tacccggtgt ataacgcagc tgcagcgagg cggtattgtt gtcaatcgtg
    32881 attaactgaa acatctgacc taatgtcatc atcccgctga tttgtttatc agcctgcttt
    32941 tgcgcttttt ccgcatccat tccttcagat aaattaagct gttttgcggt ttctgtgacg
    33001 acattaagcg gtaacttcac atcaaaattg agcgatttga tatctttgtt tgtggatgat
    33061 gaagatttgg ctgggtcggc aatactgata tccagattgg catttagttc gccgagtgcg
    33121 ttcttccatc ttaccggttg tttaatggtc ggctcacttt tttgtaataa cggtaaatac
    33181 tctttgaaca atgcagcatt cacttcttgc agagcaactt catcgtttgc taactcaggg
    33241 tgtgcagcaa gctgcttctg catcgcaatg ttatactgga tgataaattg gcgcactgct
    33301 gagggatcaa tagattcagc aatcaatgac cactcaccac taccgagttg ctgattccct
    33361 tttgttaact tatcaagttc gtaggtgaga ttgacattga tataatcttt gtctttaacg
    33421 cgatccagcc tggtttttgc aacgaaagca tcgatttggg caacgtcttc cccgtgattg
    33481 ataatgttaa tctgattaaa tttcgactcg ctttcaccaa ccggaaattt cttttcttct
    33541 aatcgcgcca gagaatcaat ggtaaaactt tttgccgtta ctttgttcat tggtgataat
    33601 tgcagaacaa tatcttcgac tttgccttca atatccaggt tagacaatga actaccatca
    33661 ccattaaaag taaactggcc ctcaccaaaa gaaaactttc catactctgc aggggcaaca
    33721 tctaatgtcg tagagaatga gccaccaaac gcgaatcgca atgtcgcttc cacgggcgat
    33781 ttatttttcg cggcgataaa tagcggttgt gtcagttcat tattcactaa gttcactttt
    33841 gccgctgcca gtgctgggat gacattacca tgcattaaca ttgtgatggg caacggaccg
    33901 tgctcaacat ccacatcaaa tacaactttc tggcctggct tgatattgag at
//
LOCUS       DAZGXZ010000045        32948 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000045 DAZGXZ010000000
VERSION     DAZGXZ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32948
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.045"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(181..1773)
                     /gene="lsrK"
                     /locus_tag="ACHKJF_002224"
     CDS             complement(181..1773)
                     /gene="lsrK"
                     /locus_tag="ACHKJF_002224"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP0949577.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1852..2805)
                     /gene="lsrR"
                     /locus_tag="ACHKJF_002225"
     CDS             complement(1852..2805)
                     /gene="lsrR"
                     /locus_tag="ACHKJF_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP0949578.1"
                     /translation="MTINDSVISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3054..4593
                     /gene="lsrA"
                     /locus_tag="ACHKJF_002226"
                     /pseudo
     CDS             3054..4593
                     /gene="lsrA"
                     /locus_tag="ACHKJF_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            4587..5615
                     /gene="lsrC"
                     /locus_tag="ACHKJF_002227"
     CDS             4587..5615
                     /gene="lsrC"
                     /locus_tag="ACHKJF_002227"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP0949579.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5615..6607
                     /gene="lsrD"
                     /locus_tag="ACHKJF_002228"
     CDS             5615..6607
                     /gene="lsrD"
                     /locus_tag="ACHKJF_002228"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP0949580.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6619..7641
                     /gene="lsrB"
                     /locus_tag="ACHKJF_002229"
     CDS             6619..7641
                     /gene="lsrB"
                     /locus_tag="ACHKJF_002229"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP0949581.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7668..8543
                     /gene="lsrF"
                     /locus_tag="ACHKJF_002230"
     CDS             7668..8543
                     /gene="lsrF"
                     /locus_tag="ACHKJF_002230"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP0949582.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVKKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8567..8857
                     /gene="lsrG"
                     /locus_tag="ACHKJF_002231"
     CDS             8567..8857
                     /gene="lsrG"
                     /locus_tag="ACHKJF_002231"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP0949583.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8914..9672
                     /gene="tam"
                     /locus_tag="ACHKJF_002232"
     CDS             8914..9672
                     /gene="tam"
                     /locus_tag="ACHKJF_002232"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP0949584.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNRLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9676..10590)
                     /locus_tag="ACHKJF_002233"
     CDS             complement(9676..10590)
                     /locus_tag="ACHKJF_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP0949585.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10798..12249)
                     /gene="uxaB"
                     /locus_tag="ACHKJF_002234"
     CDS             complement(10798..12249)
                     /gene="uxaB"
                     /locus_tag="ACHKJF_002234"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP0949586.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLCTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12476..13894)
                     /locus_tag="ACHKJF_002235"
     CDS             complement(12476..13894)
                     /locus_tag="ACHKJF_002235"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0949587.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFTIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14033..14392)
                     /locus_tag="ACHKJF_002236"
     CDS             complement(14033..14392)
                     /locus_tag="ACHKJF_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP0949588.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14392..15318)
                     /gene="glsB"
                     /locus_tag="ACHKJF_002237"
     CDS             complement(14392..15318)
                     /gene="glsB"
                     /locus_tag="ACHKJF_002237"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP0949589.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15382..16770)
                     /gene="sad"
                     /locus_tag="ACHKJF_002238"
     CDS             complement(15382..16770)
                     /gene="sad"
                     /locus_tag="ACHKJF_002238"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP0949590.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16871..17752
                     /locus_tag="ACHKJF_002239"
     CDS             16871..17752
                     /locus_tag="ACHKJF_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0949591.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17830..18944
                     /gene="yneK"
                     /locus_tag="ACHKJF_002240"
                     /pseudo
     CDS             17830..18944
                     /gene="yneK"
                     /locus_tag="ACHKJF_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            19094..20284
                     /gene="ydeA"
                     /locus_tag="ACHKJF_002241"
     CDS             19094..20284
                     /gene="ydeA"
                     /locus_tag="ACHKJF_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP0949592.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20309..20974)
                     /gene="marC"
                     /locus_tag="ACHKJF_002242"
     CDS             complement(20309..20974)
                     /gene="marC"
                     /locus_tag="ACHKJF_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP0949593.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21186..21620
                     /gene="marR"
                     /locus_tag="ACHKJF_002243"
     CDS             21186..21620
                     /gene="marR"
                     /locus_tag="ACHKJF_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP0949594.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21640..22023
                     /gene="marA"
                     /locus_tag="ACHKJF_002244"
     CDS             21640..22023
                     /gene="marA"
                     /locus_tag="ACHKJF_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP0949595.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            22055..22273
                     /gene="marB"
                     /locus_tag="ACHKJF_002245"
     CDS             22055..22273
                     /gene="marB"
                     /locus_tag="ACHKJF_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP0949596.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22330..23769)
                     /locus_tag="ACHKJF_002246"
     CDS             complement(22330..23769)
                     /locus_tag="ACHKJF_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0949597.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(23794..25467)
                     /locus_tag="ACHKJF_002247"
     CDS             complement(23794..25467)
                     /locus_tag="ACHKJF_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP0949598.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSK
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYDDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25523..25834)
                     /locus_tag="ACHKJF_002248"
     CDS             complement(25523..25834)
                     /locus_tag="ACHKJF_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP0949599.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(25862..27184)
                     /locus_tag="ACHKJF_002249"
     CDS             complement(25862..27184)
                     /locus_tag="ACHKJF_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP0949600.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLALISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEFRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(27299..27610)
                     /locus_tag="ACHKJF_002250"
     CDS             complement(27299..27610)
                     /locus_tag="ACHKJF_002250"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0949601.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            27809..28507
                     /locus_tag="ACHKJF_002251"
     CDS             27809..28507
                     /locus_tag="ACHKJF_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0949602.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(28552..29451)
                     /gene="eamA"
                     /locus_tag="ACHKJF_002252"
     CDS             complement(28552..29451)
                     /gene="eamA"
                     /locus_tag="ACHKJF_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP0949603.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            29646..30833
                     /gene="ydeE"
                     /locus_tag="ACHKJF_002253"
     CDS             29646..30833
                     /gene="ydeE"
                     /locus_tag="ACHKJF_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP0949604.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            30960..31055
                     /gene="mgtS"
                     /locus_tag="ACHKJF_002254"
     CDS             30960..31055
                     /gene="mgtS"
                     /locus_tag="ACHKJF_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP0949605.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            31055..31159
                     /gene="mgtT"
                     /locus_tag="ACHKJF_002255"
     CDS             31055..31159
                     /gene="mgtT"
                     /locus_tag="ACHKJF_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP0949606.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(31274..32164)
                     /gene="dgcZ"
                     /locus_tag="ACHKJF_002256"
     CDS             complement(31274..32164)
                     /gene="dgcZ"
                     /locus_tag="ACHKJF_002256"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP0949607.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTSALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(32419..32811)
                     /gene="ydeI"
                     /locus_tag="ACHKJF_002257"
     CDS             complement(32419..32811)
                     /gene="ydeI"
                     /locus_tag="ACHKJF_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP0949608.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         8639 a         8156 c         7624 g         8529 t
ORIGIN      
        1 ataagggttg tgcttaatgc tttaagaaaa atagcaattt tcccttgaat atagtactgg
       61 tgatggaacg atgaatctgc aagctggctt ttaacaagcc agctctaaaa gaagggaaat
      121 aagaataact atactcaaaa actaacagcc acggtcatca tgatgtggct gtcaatgaaa
      181 ctataaccca ggcgctttcc ataacgacgt cgtcagtcca tgatcaacca gccccagctg
      241 atcctgataa actgcctgcc acttatcgcg tgaatcctga taaagttcat gcttttccgg
      301 gtctggtgtg tgcgtccgtt cccagcgaac caggcgttct ccggtttctg ccattgatga
      361 aaaaattccg gcaccgacgc cagctgcaat ggcacatcct aatgcagtgg cttctttgac
      421 caccggaata ttgacgggta atcccgagac atcagcgaga atttgactcc ataatttccc
      481 ttttgaacct ccgcctgcaa agactaacga tgaaggatga atattcgaga aatcagcaat
      541 ttgctgcaag ttacacgctg atacaatcgc cgcattttct tccagcgcac ggaacaatgt
      601 cgctttgtta catttatccg ggtcaatgga caagttaata aaggaaggcg cagcgtgata
      661 ccaggtttta aagcgcattc tgtcggagaa gatcggcatt acgccccacg acccaggcgg
      721 cacccgactg gccatctctt ccagcagcgt ataggtgtcg atgcctaaac gttcggcaat
      781 cagtttttct tcggcacaga aagcatcgcg gaaccagcgc atggtgagtc cggtaaaaaa
      841 gcttatagat tcagcttgta ccatgccagg aataacatga ggattaacgc gcacgttcat
      901 ttctgggtct gtcaccggcg cggctaaatt tacaacttgc tgccagaatg tgccgccaag
      961 aaccgcggtt tgtgccggac gcacaacgcc taacccaagg caaccaagct gcacgtcgcc
     1021 tcctccaacg accaccggag tgcccgcctt cagaccgcag agttccgccg cttgtgaact
     1081 taccacgccc agcaatgtgc cggtttcttt gacaggagaa agaatatcgg cacgtaggcc
     1141 agccatatcc agcaatgcag gtttccagtc acgggtggtt agatcaagaa gtcccgtggt
     1201 gccagcgtta gagggatcca ccgccagttc gccgctgagc atataggcca gccagtcgct
     1261 gatcatggtg atggttgatg cctgacggta aatatcggaa cgatggtgcg ccagccaaag
     1321 taatctgggg atggcactta aagccagtgt ttgtccggtc gcgcgataaa cttcgttttc
     1381 aaaggtattg ttgtgcagtt ctttaagttc gctaacttcg cgtgccgctc tggcatccac
     1441 attggcgcag gcccagatcg gggctccttc attattatat aaaacaatgc cttcacgcat
     1501 cgaacatgcc gaaacggcag cgatatactc cggggctatg ccggcgttgt gcagcgcctg
     1561 gcgcatacac tcacacgcca gttgccagtt tttgttgaga tcaaattcca tagaaccagg
     1621 aacgtccggt actgccaggt gccgccactc cgcctgtccc actgctattt gattgccttc
     1681 caggtcgaat atcacagccc gaatacttcc ggtacctgca tccagcgcca tcaggtaata
     1741 ctttgattct gaaggggtaa agagtcgagc cattagatta tcctcggctt atgcgttctg
     1801 tctgttcctc tatacgttct ccatcattcc cggtaataag gtcatgcaaa tttaactacg
     1861 taaaatcgcc gctgctgtgt cctgatcggt aaccagtgcg ttgatataac cgcctttcat
     1921 tgcagcggca attgcttcgg ctttattttc tccccctgcc acgccaaccc ggacgggtat
     1981 ggtcttcagc gagcttaaag gtaagccaat cagttcgtta tgtattttaa tatccgtgac
     2041 aacgtcacct tttgcatcaa aaaagtagcc taaaatgtcg ccaaccgccc cttttcgacc
     2101 aatcattaac tgttcgccct ggctgatata accggagcga atgatcgtcg cgtcgtcctg
     2161 ttgactgacg gccccaatgc cgacaatcgc cacatccgct gcttgcgcgg ctaacagaac
     2221 atctttgacg caattttcat tttttagcgt acgggcaatg tcagcggagg atgcccgcaa
     2281 cggagccgga ataatattca cactgcacgc cgcgttaagc tgcccgatac ccgtcatata
     2341 agaaccgacg ccaccggaga gcgtgaccag gcgaatttgc tgtgacgaaa taaaaccact
     2401 taagcgttgc agcgtattca tagttgcctc gccaaaacca atcgccagca tctgttgtgg
     2461 ttgaagtaaa ctcatcaaca tatgcgccgc gcctatcccc agtcgcccgc cgacatcagc
     2521 atccgcaagc ccagggatca cccggacatg ttgcagcgaa aactgacgac gtaattgtgt
     2581 ttcatattcc agacagcctt caaagcgaga attaatctgc acgcgaataa tgccggactg
     2641 atgccctttc tccagtaatc gcgacacttt caaacgggtc aggccgagac gatcgctgat
     2701 ctcactctgg gtcaacccgt cgtggtagta aaaccacgcg atccgcgcga cctgttcttc
     2761 ttcacacatt ccctgttctg aaattaccga atcgttgatt gtcataattc attcttcact
     2821 ttgaacatat ttaaatcttt aatgcaattg ttcagttctt gcgcatttat atctgtgatg
     2881 gcaaccacag tttgactcta cgagcatgaa caaacgcaac cgtgaaaatc aaaatagcat
     2941 aaattgtgat ctattcgtcg gaaatatgtg caatgtccac ctaaggttat gaacaaatta
     3001 aaagcagaaa tacatttgtt caaaactcac ctgcaaaact gaacggggga aatatgcaaa
     3061 cgagtgatac ccgcgcgtta ccgctacttt gcgcccgctc ggtttataaa cagtattcag
     3121 gggtcaatgt cctgaaaggc atcgatttta cgttgcatca gggggaggtc cacgccctgc
     3181 tcggcggcaa tggtgccggt aaatcgacgt taatgaagat tattgccggt attacccctg
     3241 ctgatagcgg tacgctggag attgggggca acaactacgt cagattaacg ccagttcatg
     3301 ctcatcagct gggtatttat ctcgttcccc aggaaccgct gcttttccca agcctgtcga
     3361 taaaagaaaa catcctgttt gggctggcaa aaaaacagct ctccatgcag aaaatgaaga
     3421 acttgctggc ggcgctgggc tgccagtttg atctgcatag tctggcagga tcgctggatg
     3481 tcgccgatcg ccaaatggtg gaaatcctcc gcgggctgat gcgcgactcg cggattctga
     3541 tcctcgatga acctaccgcc tcgcttaccc ctgcggaaac cgaacgcttg tttagtcgct
     3601 tgcaagagct gcttgctact ggcgtgggta ttgtttttat ctcgcataag ctgccggaaa
     3661 ttcgccagat tgccgatcga attagcgtga tgcgcgacgg aaccatcgcc ttaagcggca
     3721 aaaccagcga actgtctacc gacgacatta ttcaggccat tacgccagcg gtacgggaaa
     3781 aatcgctctc tgccagccaa aaattatggc tggagttacc tggtaaccgc ccacaacatg
     3841 ctgccggaac gccggtgctg acactggaaa atctgaccgg cgaaggtttc aggaatgtca
     3901 gcctgacgct caatgccgga gaaattctgg gcctggctgg gctggtggga gccggacgca
     3961 cagaactggc cgagacgctc tatggtctgc gtactttgcg tggcggacgc attatgctga
     4021 atggtaaaga gatcaataaa ttatccactg gagaacgttt actgcgcggt ctggtttatc
     4081 tgccggaaga tcgccagtca tccggactga atctcgatgc ttcgctggcc tggaacgtct
     4141 gcgcccttac tcataacctt cgtggattct gggcgaaaac cgcgaaagat aatgccaccc
     4201 tggaacgtta tcgtccgtcg ggcgctgaat attaaattta accaaccgga acaagctgca
     4261 cggacattat ccggtggcaa ccagcaaaaa atcctcattg ccaaatgcct ggaagcttcg
     4321 ccgcaagtat tgattgtcga tgagccgacg cgcggcgtgg atgtctcggc acgtaatgat
     4381 atctaccagc tgttgcgcag catcgccgca cagaatgtgg ctgtgctgct tatctcctct
     4441 gatctggaag agatcgaact gatgacagat cgtgtgtatg tgatgcatca gggcgaaatt
     4501 acccactctg cactgaccgg gcgcgatatt aatgtcgaga ctattatgcg cgttgccttc
     4561 ggcgatagtc agcgtcagga ggcatcatgc tgaagtttat tcagaacaac cgtgaaatca
     4621 cggcattgct ggcggtggtg ctgctgtttg tattacccgg ttttctcgac cgccagtatt
     4681 taagtgtgca aacgctgacc atggtttata gcagcgcgca aatcctgatc ctgctggcaa
     4741 tgggcgcgac gctggtaatg cttacgcgca atattgatgt ttcagtgggt tcgattaccg
     4801 gaatgtgcgc ggtgctgttg gggatgttac tgaacgcagg atattcacta cctgttgcct
     4861 gtgtcgcgac tttactgctt ggtttgctcg cgggattttt caacggtgtc ctggtcgcgt
     4921 ggctaaagat ccctgccatt gttgccaccc ttggcacgtt agggttgtac agaggcatca
     4981 tgttgctgtg gactggcggt aaatggattg aagggttacc cgccgaactg aaacagctct
     5041 ccgcaccgct gctgtttggc gtttccgcga ttggttggtt gacgataatt ctggtggcat
     5101 ttatggcctg gctgctggca aagacggcgt ttggacgcag tttttatgcc acgggcgata
     5161 atttacaggg cgctcgtcaa ctgggtgttc gtacggaagc cattcgcatt gtggcatttt
     5221 cgttgaacgg ctgcatggcg gcactggcgg gaattgtgtt tgcttcgcag attggtttta
     5281 tccccaacca gaccggtacc gggctggaga tgaaagcaat tgcagcctgc gtgctgggcg
     5341 gcattagttt gctcggcggt tccggtgcga tcatcggtgc ggtactcggc gcatggttcc
     5401 tgacgcagat cgatagcgta ctggtgctgt tgcgcattcc ggcatggtgg aacgatttta
     5461 tcgcgggcct cgtgctgctg gcggtgctgg tgtttgatgg acgcctgcgt tgtgcgctgg
     5521 aacgtaatct acggcggcaa aaatatgccc gctttatgac gccaccgcca tccgttaaac
     5581 ccgcttcgtc aggtaaaaaa cgggaggccg cataatgcgt attcgctacg gttgggaact
     5641 ggctcttgcc gcactgctcg ttattgagat tgtcgcattt ggtgcaatta acccgcgaat
     5701 gttagatctc aatatgttgc tgttcagcac cagtgacttt atctgcattg gcattgtcgc
     5761 cctaccgctg acgatggtga ttgtcagtgg cgggatcgat atttcgtttg gttcgaccat
     5821 cggcctctgc gccattgcat tgggcgtact gtttcaaagt ggtgtgccga tgccgctggc
     5881 gatactcctg accttactgc tcggcgcatt gtgcgggctg atcaacgccg gattaattat
     5941 ctataccaaa gttaacccgc tggtgattac gcttggcacg ctgtatctgt ttgccggaag
     6001 cgctctgctg ctttccggta tggccggagc gacggggtac gaaggtattg gtggattccc
     6061 gatggcgttt acagatttcg ctaacctgga tgtgctggga ctccccgttc cgctgattat
     6121 cttcctgata tgtctcctcg ttttctggct ctggctgcat aaaacccatg ccggacgtaa
     6181 tgtgtttttg attgggcaaa gcccgcgcgt ggcgctttat agcgcgattc cagttaaccg
     6241 caccttatgt gcgctctatg ccatgacggg gctggcgtct gcggtcgccg ctgtgctgct
     6301 ggtatcgtat tttggttcag cacgttccga tctcggtgcg tcgtttctga tgcccgccat
     6361 caccgccgtg gtgcttggcg gtgccaatat ttatggtggt tccggttcca ttatcggcac
     6421 cgccattgcg gttttattag tgggatattt gcaacaaggt ttgcaaatgg caggagtgcc
     6481 aaatcaggtg tccagcgccc tttccggtgc gctacttatc gtcgttgtcg taggtcgttc
     6541 cgttagcctg catcgccagc aaattaaaga gtggctggcg cgtcgggcca ataacccatt
     6601 gccataaagg atatcttcat gacacttcat cgctttaaga aaatcgcctt acttagcgct
     6661 cttggcattg ccgcaatctc tatgaatgtg caggccgcag agcgtattgc atttattccc
     6721 aaactggttg gcgtgggatt ttttaccagc ggtggcaacg gcgcacaaca agcgggtaaa
     6781 gagctgagcg ttgatgtgac ctacgacggg ccgacagaac ccagtgtttc tggtcaggta
     6841 cagttgatta ataacttcgt caatcaaggt tataacgcca ttatcgtttc tgcggtttcg
     6901 cctgatggct tgtgtccggc actgaaacgc gccatgcaac gtggtgtgag agtgctgacc
     6961 tgggactctg atactaaacc ggagtgccgc tcttactaca ttaatcaggg aacgcccgcc
     7021 cagttgggag gtatgttggt ggatatggcg gcgcgtcagg tgaataaaga caaagccaaa
     7081 gtcgcgtttt tctactcaag ccccaccgtt acggaccaaa accagtgggt gaaagaagcg
     7141 aaagcgaaaa tcgccaaaga gcatcctggc tgggaaattg tcactacgca gtttggctat
     7201 aacgatgcca ctaaatcatt acaaaccgca gaaggaatat taaaagcgta tagcgatctc
     7261 gacgccatta tcgcccccga cgccaacgcc ctgcccgctg ccgcacaagc cgcagaaaac
     7321 ctgaaaaatg acaaagttgc gattgtcgga ttcagtacgc caaacgtgat gcgtccatat
     7381 gtggaacgcg gcacggtgaa agaatttggc ctgtgggatg tggttcagca aggcaaaatt
     7441 tcagtgtatg tcgcggatgc attattgaaa aaaggatcaa tgaaaacggg cgacaagctg
     7501 gatatccagg gcgtaggtca ggttgaagtc tcgccaaaca gcgttcaggg ctatgactac
     7561 gaagcggatg gtaatggcat cgtactgtta ccggagcgcg tgatattcaa caaagagaat
     7621 atcggcaaat acgatttctg atgtgcatta cttaaccgga gtaagttatg gcagatttag
     7681 acgatattaa agatggtaaa gattttcgta ccgatcaacc gcaacaaaat atccctttta
     7741 ccctgaaagg ttgcggtgcg ctggattggg gaatgcagtc acgcttatcg cggatattta
     7801 atccgaaaac gggtaatacc gtgatgctgg cctttgatca tggttatttt caggggccga
     7861 ctaccggact tgaacgcatt gatataaata tcgccccgct gtttgaacat gccgatgtat
     7921 taatgtgtac gcgcggcatt ttgcgcagcg tagttccccc agcgaccaat aagccggtgg
     7981 tactgcgggc gtcaggtgcg aactctattc tggcggaatt aagtaatgaa gccgtggcgt
     8041 tatcgatgga tgatgccgtg cgcctgaaca gttgcgcggt ggcggcgcag gtttatatcg
     8101 gcagcgaata tgaacatcag tcgatcaaaa atattattca gctggttgat gccggaatga
     8161 aagtgggaat gccgaccatg gccgtgactg gcgtgggcaa agatatggtg cgcgatcagc
     8221 gttatttctc gctcgcgact cgaatcgccg ctgaaatggg ggcgcaaatt atcaaaacct
     8281 attatgtcaa aaaaggtttt gaacggattg ttgccggatg tccggtaccc attgttattg
     8341 ctggcggtaa aaaattaccg gagcgcgagg cgctggaaat gtgctggcag gctatcgatc
     8401 agggcgcttc tggtgtggat atggggcgta atattttcca gtctgaccat ccggtggcga
     8461 tgatgaaagc cgtacaggcg gtggttcacc ataacgaaac ggctgatcgg gcatatgaac
     8521 tctatctgag tgaaaaacag taactgcgga tttaaggaga agaattatgc acgtcacact
     8581 ggttgaaatt aacgttcatg aagacaagat tgacgagttt atcgaagttt ttcgccagaa
     8641 ccacctgggc tctgtacagg aagaaggcaa tttgcgcttc gatgtcttac aggacccaga
     8701 agtgaattcg cgcttttata tttacgaagc ctataaagat gaagacgcag tggcattcca
     8761 taaaaccacg cctcactaca aaacctgtgt cgcgaaactg gaatctttaa tgaccgggcc
     8821 gcgtaaaaaa cgtctgttca atgggttgat gccgtgaggc gaatttatca tttttatcta
     8881 caattggggt aacgcgctga cgggagtaaa aatatgtctg actggaaccc ctctttatat
     8941 ctgcactttg ccgctgaacg atcgcgtccg gcggtggagc tgcttgccag agtgccgctg
     9001 gagaatatca aatacgttgc cgatcttggc tgtggcccag gtaacagcac cgccctgctg
     9061 catcaacgtt ggcctgcggc caggataaca ggcatcgact cgtctccggc gatgattgct
     9121 gaagcgcgca gtgctttgcc agactgccag ttcgtggaag cagacatccg caactggcaa
     9181 ccggaacagg cactcgatct gatttttgct aatgcctcac tgcaatggct gcccgaccac
     9241 tacgaattgt ttcctcatct ggtttcttta cttaatccgc agggcgtgct ggcagtacag
     9301 atgccagata acaggctgga gccgacccat gtgctcatgc gcgaagttgc ctgggaacaa
     9361 aactacccgg atcgcgggcg tgagccgttg gctggcgttc atgcttacta cgatattttg
     9421 agcgaagccg gatgtgaggt cgatatctgg cgaaccacct actatcacca gatgccgtca
     9481 caccaggcga ttatcgattg ggtgactgcc actggattac gtccgtggtt acaggatctg
     9541 accgagagcg aacagcagct ttttcttaag cgctaccatc agatgctgga agagcagtat
     9601 ccactgcaag agaacggaca gatactgctg gcatttccgc gtctttttat tgttgcccgg
     9661 cgtatggagt aaatatcacg tcagctggta atgacgatcg ggaagaattt ttgctggaat
     9721 ttcggcttca tcgttcatct gtaacaggtc aatttcaata gcgttgcaga tggcatccag
     9781 tggtaaatca ttgttttcag taccgaacgg atcttccagt tcttccgcca gacaatccag
     9841 cgaaataaaa gtgtaggaaa tcagcacaga gataaaaggc gtcatgtaat gcaggtccac
     9901 gaccagcgcg aacggcagca tgatacaaaa cagataaacg gtacgatgca aaatcagggt
     9961 gtaggcaaag ggaattggcg tataggcaat gcgctcgcat cctgccagga ccgctgaaat
    10021 atcattaagc cgatcgttga ggctaataaa caggatatct gaaagctgcc cattgcggcg
    10081 ctgaaccgcc aaccactccc ccattattaa caagatacgg ttagctggag agttcgaagc
    10141 cagtacaggc tgaagatctt cagtcttgag ataatgagcc agcacttccg cctgtggctg
    10201 tttgcgtaat gtcatgcgta aacagtgggc gaaggcgatt tgcaaccggg caaactccct
    10261 tacacttgcc gaatccggca atgtcgtttt tacctcgcgc agtaacgacc gtgaggcaat
    10321 catcaactgc ccccaaagtt ttcgcgcttc aacgtaacgg gcgtacccgg cattattacg
    10381 aaaaccaaga aaaatggcga tggcgacacc gagaatgctg aacggtgcga gggtgaattt
    10441 gatgcccaga tgcgtgtacc agggcagcat gaaaataaca gcgatagaaa agagaaaatt
    10501 gagtagtaag cgcgatgata tcttggataa tactgagccg tgccagacaa aaatacggcg
    10561 cagccagtgt tgttgtggac gaacaatcat ggttatcttc aggcgtgaaa aagtcgccct
    10621 attaaacgtg attacgatca cattctcaag acgctactta caaattacct acaagcttaa
    10681 caacattaac aggcgctgca tgtggcatct gttaatgata aaggcacata ggatgttgta
    10741 actaatatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac ggtgttatta
    10801 gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca ggtcattagc
    10861 aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt ctttttctgc
    10921 caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct ggctccagag
    10981 ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg
    11041 ctcaccacga tagaaggcaa tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt
    11101 gccttttgcc ttctgccctg ccagcagctg cggcaggatg cgagtgcgga acttggtcat
    11161 accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga aacgcccggt
    11221 gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg gaataatttc
    11281 ttcgtagata gctttttcaa cgaatgcgca aatttcagca tcgttcatcg cttcacctac
    11341 ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat
    11401 tgccactttg cgttctttgt acggtttaat atcgtcaaca atcagcacgt tgagcggata
    11461 tttgtccaga cgcagttcag tcgctaagga tttcggtccc tgaatcacaa acaggtaaaa
    11521 gtgttcagcg gtgtcgagaa aaccatcgtg ataacccagc tcttcttcca gtttagccac
    11581 ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga agctgttagc
    11641 ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat agcgcagcac
    11701 cagttcacgc aacgcgtcgc cattgtagtc gatcaactca cacgggatga tgatccaacc
    11761 tttatccagc gcaccgttga aatggctaaa acgttcgaac agcagacggg tcagttttgc
    11821 cggataactt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc
    11881 ttcggtggta ttagagaaaa caaagcgcat ttccgggttg tgagccagtt tcaggaattc
    11941 atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac gcgcgtcgct
    12001 gaccgcttcc cccttctcat tcaggccacg gataatggtg gtatacagac catcctgcgt
    12061 gcacagtgac ggtgggaatg aagtttcaat cggacgaaca acgaccacac cagaattcag
    12121 atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc
    12181 ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc gacgatttag
    12241 tgttttcaca atgggttccc ttctgattag tcatacaacc tgtttgaatt ggtacgacag
    12301 gttagcaaac tttaatacgc cgaccccctg ttttgatcaa ctcctgatga ttaatgagca
    12361 gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg ccttttcctg
    12421 gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa agatgtcaga
    12481 caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc ttcgagcgat
    12541 acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc gttcggtacg
    12601 atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt tgttgccagg
    12661 tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga ccatcgacag
    12721 gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc accagcccct
    12781 tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta tcgccacatt
    12841 catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc atgaccgtca
    12901 gatgttgtgt ctgtttcagc gatggacttt tcagcgcctc ataaagaccg gaccgtgagt
    12961 aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt agcgaattga
    13021 tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca ataccgagac
    13081 gtgcggagaa catttccgga atggtgaacg gcgaaccgac cgagatatca atcaccgaat
    13141 ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat gttacctgcg
    13201 gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga ctccccgctc
    13261 cgccaatgac taccgaggca ataaccgaca caataagcgc gatggcgggc atcatctgtt
    13321 ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa ggcacgatca
    13381 acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag aaatccagac
    13441 tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg gcaatcagac
    13501 agtaattaaa tagccgtaag gcacttactg gctcatactt atttttcccc agacgcttat
    13561 cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa ttgataacca
    13621 gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc acataactga
    13681 tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc gccattacgc
    13741 cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca ccaataaaat
    13801 agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga ccttcctgga
    13861 acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata caattgcggg
    13921 tggcaattat ctcaacgtaa taagtaaatt ctcataaagg atagcatgtt cgctaacagc
    13981 ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat tctcatggct
    14041 gattcatctg tacaaccagc cagtgataga ttaccgccac gatataacgt tgttgttctt
    14101 cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg cgacagcagg
    14161 tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt tgcttaccgt
    14221 cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca tgttgttcga
    14281 tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga cgaaagcgag
    14341 agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc attaataaac
    14401 cgaacgccct aactgtttcg tcaattgttc aaggacggca atacccgcaa gcgagttacc
    14461 tgcatcatcc agttccggac tccagacagc gatggccatt tcatgcggaa caatcgccac
    14521 aataccgcca ccaacgccag atttcgccgg tagccccacc cgccaggcaa actcccccgc
    14581 gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt
    14641 caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga caaacgtccg
    14701 ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt gcagaacggt
    14761 tgtcacatca tgatggaaat tgccaaacga cttcatcagc caggcgatag ccgcatttcg
    14821 cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat cagacacacc
    14881 gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttaatc gcccttgcaa
    14941 catatcgcaa accaccagcg caccagcatt aataaacgga ttacgcggaa taccctgctc
    15001 catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc cgacgcgttg
    15061 ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca ctttggaaat
    15121 agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt
    15181 acagatagca atgcccaatc gggaaccgtc tactgtagcc agcgccggaa tataatccgc
    15241 gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta aaattgcatt
    15301 atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat
    15361 atcagagctg aatatgtcgc gtcagatccg gtctttccac accgtctgga tattacagaa
    15421 ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca cgccaccaaa
    15481 ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc attccagacg
    15541 tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa
    15601 ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaacgg tgattgccgc
    15661 aacagggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat tcgccagaac
    15721 cgttggcgga tagtagttac ctgccccagc catcttttcc ccgcccagta acaaacgcgc
    15781 accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat caaaacgagc
    15841 cattggtccg agagcattct cttcatcacg gggatcgccc attttcaagg ctgccgcagc
    15901 tgccacaaaa cgttcggtaa atgcagaagc aattccctct tcgataataa agcgttttgc
    15961 cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt taaccgccag
    16021 ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt ccagtacgca
    16081 ttttttcagt gccgctccag cctgtgcgcc aatagccgct cccgcacgaa cacttccggt
    16141 caccgtgaca gcagcaatgc gcgaatcttt aatcatttga ctgacaccgt cgttgtcggc
    16201 attcagccag ccatatacgc cttgcgggat tcccgcatct ttaaacacct gggcaatgag
    16261 ctgagcacag cccatcacat tcggcgcatg tttaagtaag tagccgttac ctgcaagaat
    16321 gatgggaacc gcgccacgca tcacctgcca taacggaaag ttccacggca taatcgccag
    16381 aatcgtcccc aacggtcgat actcaataac tgcctgctga ttttccacca gcgtaggttc
    16441 cgccttcagc attgccggac catgttctgc ataccagtca cacaaattcg ccgatttcgc
    16501 cacttcagcg cgcgcctgat tgattggttt gcccatttca cgggtgatca tttgcgccat
    16561 ttcttcgcta cgggcgcgca gagccttacc gataccacgc agtttttcag cacgataatc
    16621 tatatttgtc tcgcgccagt cgcgaaagcc tgctgccgcc agctgaagtg cgttttcgat
    16681 atcgttagcg ccagcccacg gcagcacaga aagttgttca cccgtggcag gatttattga
    16741 aattgcatga gttgccggag taatggtcat cggggtatct cctttatgag tcatggtatg
    16801 aagatacgca gatttactct tgctttaaaa tgaataatat taagccactt attcacgaat
    16861 cgagaatgtt atggatctga cccaactgga gatgttcaac gcggttgccg aagctggcag
    16921 cattacccag gctgcagcaa aagtgcatcg cgtaccgtcg aatctcacta cccgtctacg
    16981 ccagctggaa acagaactgg gggtagatct gtttattcgc gagaatcagc gtttacgtct
    17041 ctctcctgcc gggcataact ttttacgcta tagccaacaa attctcgcgt tagtggatga
    17101 agcgcggagc gttgtcgctg gcgatgagcc gcaaggttta ttttctcttg gttcgctgga
    17161 aagcaccgca gcagtgcgca ttccggccac gctggcggaa ttcaaccatc gttatcccaa
    17221 aattcagttt tcactttcca ccggcccttc cggcactatg ctggaaggag tgctggaggg
    17281 aaaactgaat gcggcattta ttgatgggcc cattaaccat actgccattg acgggatacc
    17341 ggtataccgc gaggagctga tgatcgtcac gccacaaggt catgcgccag taatccgcgc
    17401 cagtcaggtt aacggcagta acatttatgc cttccgcgcc aattgttcgt atcgtcgcca
    17461 cttcgagagc tggtttcatg ctgacggtgc cgctccggga actattcacg agatggagtc
    17521 gtatcacggg atgttggcct gtgtggtcgc aggagcaggc attgcgctta ttccacgctc
    17581 tatgctggaa agtatgccgg ggcatcacca ggttgaagcg tggccgttag ctgagcaatg
    17641 gcgttggtta acaacctggc tggtctggcg tcgtggtgcg aaaacgcgtc cgcttgaggc
    17701 atttattcaa ctactggatg tgcctgactc ggcaaaacag ggttaccaat gagctatttt
    17761 tgatagttct tgcagctcaa atgccctata tagtgatgtt ccgatgactt atgactatat
    17821 ggggcaaata tggttacgcc agtaagcatc agtaattaca tatctcttcc tgatgatttt
    17881 cccgtacgta atattgcacc tcaggtaaaa gaagttttaa aggattttat tgatgcactc
    17941 agtaccataa tatgtgatga agagtggcgc acgtctttaa acaccaactc agccacaaaa
    18001 aagatattta acaaccttga taacttatca tatattcaga ggacttcttt tcggggtaac
    18061 gacacactat acaatgaaaa ggttcaattt aaactcactt attccgtcaa gaatggaaga
    18121 cacaaagaaa atattgaatt tcaggtagta ataaatttaa gccctattta tttagataat
    18181 tttcgccatg atggagaaat taatattttt gcgctcccca cccaaagcct gtcactatgg
    18241 ggcgcgtatt tcagaccggc gtcgagcgtg tgctatttct gtttctgaat gattttattg
    18301 aacaatttcc aatgatcaac cttggcgttc ccatcaaaag agcgcataca ccacatattg
    18361 aacccctgcc tccggatcgc cataccgctg cagattactt acgccagttt gatttgcttg
    18421 tcctgaattt tatctctcgc ggtaattttg tcatactccc ccgattatgg agtaactctg
    18481 aggttcacag atggttcgtc aataaagatc ctaacttgat caccgccatt ctcgacataa
    18541 cagacggcga attaaaagag gatttattgc aaagcctgat ggattcatta ggttctaaca
    18601 aacatgtact acccgaagtc tgcatctgct ttttatccct tttagcagaa caagaatctc
    18661 ctcattttca ggactttttt ttatttttcg ccaatatgtt attgcactat caccaattta
    18721 tgaatcccaa tgaaagtgat ctgaatgacg tgttgatgcc agcatcatta agtgatgata
    18781 aaattatcaa acatatggcg cgcaggaccc tcaaactgtt tgtaaaaaat gaaacgccgc
    18841 caaaagttac tcacgaagac ctggtgaaaa acaggcctcg ctcccctgtc agaccaccta
    18901 tacccgcaac cgccaaaacg ccagacctcc ctgaacgtca ttaaaccgtg atgttaccga
    18961 ctctctgacg cggaacagaa tcagcgtcag agaaacggaa aacgcgatcc agatcacaaa
    19021 tgcattgtat tcacatcatt aaccgtttta agatcatttc atcacttttt cgcaactcac
    19081 ccgataatct gttatgacaa caaacactgt ttcccgcaaa gtggcgtggc tacgggtcgt
    19141 tacgctggca gtcgccgcct tcatcttcaa caccaccgaa tttgtccctg ttggcctgct
    19201 ctctgacatt gcgcaaagtt ttcacatgca aaccgctcag gtcggcatca tgttgaccat
    19261 ttacgcatgg gtagtagcgc taatgtcatt gccttttatg ttaatgacca gtcaggttga
    19321 acggcgcaaa ttactgatct gcctgtttgt ggtgtttatt gccagccacg tactgtcgtt
    19381 tttgtcgtgg agctttaccg ttctggtgat cagtcgcatt ggtgtggctt ttgcacatgc
    19441 gattttctgg tcgattacgg catctctggc gatccgtatg gctccggccg ggaagcgagc
    19501 acaggcattg agtttaattg ccaccggtac agcactggcg atggtcttag gtttacctct
    19561 cgggcgcatt gtgggccagt atttcggttg gcgaatgacc ttcttcgcga ttggtattgg
    19621 ggcgcttatc acccttttgt gcctgattaa gttacttccc ttactgccca gtgagcattc
    19681 cggttcattg aaaagcctcc cgctattgtt ccgccgcccg gcattgatga gcatttattt
    19741 gttaactgtg gtggttgtca ccgcccatta cacggcatac agctatatcg agccttttgt
    19801 acaaaacatt gcgggattca gcgccaactt tgccacggca ttactgttat tactcggtgg
    19861 tgcgggcatt attggcagcg tgattttcgg taaactgggt aatcagtatg cgtctgcgtt
    19921 ggtgagtacg gcgattgcgc tgttgctggt gtgcctggca ttgctgttac ctgcggcgaa
    19981 cagtgaaata cacctcgggg tgctgagtat tttctggggg atcgcgatga tgatcatcgg
    20041 gcttggtatg caggttaaag tgctggcgct ggcaccagat gctaccgacg tcgcgatggc
    20101 gctattctcc ggcatattta atattggaat cggggcgggt gcgttggtag gtaatcaggt
    20161 gagtttgcac tggtcaatgt cgatgattgg ttatgtgggc gcggtgcctg cttttgccgc
    20221 gttaatttgg tcaatcatta tatttcgccg ctggccagtg acactcgaag aacagacgca
    20281 atagttgaaa ggcccattcg ggcctttttt aatggtacgt tttaatgatt tccaggatgc
    20341 cgttaataat aaactgcaca cccatacata ccagcaggaa tcccatcaga cgggagatcg
    20401 cttcaatgcc acccttgccc accagccgca taattgcgcc ggagctgcgt aggcttcccc
    20461 acaaaataac cgcaaccagg aaaaagatca gcggcggcgc aaccatcagt acccaatcag
    20521 cgaaggttga actctgacgc actgtggacg ccgagctaat aatcatcgct atggttcccg
    20581 gaccagcggt acttggcatt gctagcggca caaaggcaat attggcactg ggttcatctt
    20641 ccagctcttc cgacttgctt ttcgcctccg gtgaatcaat cgctttctgt tgcggaaaga
    20701 gcatccgaaa accgataaac gcgacgatta agccgcctgc aattcgcaga ccgggaatcg
    20761 aaatgccaaa tgtatccatc accagttgcc cggcgtaata cgccaccatc atgatggcaa
    20821 atacgtacac cgaggccatc aacgactgac gattacgttc ggcactgttc atgttgcctg
    20881 ccaggccaag aaatagcgcg acagttgtta atgggttagc taacggcagc aacaccacca
    20941 gccccaggcc aattgcttta aacaaatcta acattggtgg ttgttatcct gtgtagctgg
    21001 gttatcagcg aaaagtataa ggggtaaaca aggataaagt gtcactcttt agctaacctt
    21061 gcatcgcatt gaccaaaact tgaaccgatt tagcaaaacg tggcatcggt caattcattc
    21121 atttgactta tacttgcctg ggcaatatta tcccctgcaa ctaattactt gccagggcaa
    21181 ctaatgtgaa aagtaccagc gatctgttca atgaaattat tccattgggt cgcttaatcc
    21241 atatggttaa tcagaagaaa gatcgtctgc ttaacgagta tctgtctccg ctggatatta
    21301 ccgcggcaca gtttaaggtg ctctgctcta tccgctgcgc ggcgtgtatt actccggttg
    21361 aactgaaaaa agtgttgtcg gtcgacctgg gagcactcac ccgtatgctg gatcgcctgg
    21421 tctgtaaagg ctgggttgaa aggttgccga acccgaatga taagcgcggc gtattggtaa
    21481 aacttaccac cagcggcgcg gcaatatgcg aacaatgcca tcaattagtt ggccaggacc
    21541 tgcatcaaga attaacaaaa aacctgacgg cggacgaagt ggcaacactt gagcatttgc
    21601 ttaagaaagt cctgccgtaa acaaaaaaga ggtatgacga tgtccagacg caatactgac
    21661 gctattacca ttcatagcat tttggactgg atcgaggaca acctggaatc gccactgtca
    21721 ctggagaaag tgtcagagcg ttcaggttac tctaaatggc acctgcaacg gatgtttaaa
    21781 aaagaaaccg gtcattcatt aggccaatac atccgcagcc gtaagatgac ggaaatcgcg
    21841 caaaagctga aggaaagtaa cgagccgata ctctatctgg cagaacgata tggcttcgag
    21901 tcgcaacaaa ctctgacccg aaccttcaaa aattactttg atgttccgcc gcataaatac
    21961 cggatgacca atatgcaggg cgaatcgcgc tttttacatc cattaaatca ttacaacagc
    22021 tagttgataa cgtgacaacg tcactgaggc aatcatgaaa ccactttcat ccgcaatagc
    22081 agctgcgctt attctctttt ccgcgcaggg cgttgcggaa caaaccacgc agccagttgt
    22141 tacttcttgt gccaatgtcg tggttgttcc cccatcgcag gaacaaccac cgtttgattt
    22201 aaatcacatg ggtactggca gtgataagtc ggatgcgctc ggcgtgccct attataatca
    22261 acacgctatg tagttattgt caggctctga tcacagagcc tgacatctcc gtaatatctt
    22321 cctcaagtct caaatatttt cgccattgtt ggcgatgaga tcccgatacc agtaaaacga
    22381 cgctttacgt atccgttcca gcgttccctt cccttcgttg tctttatcga cataaatcat
    22441 tccgtagcgc tttttcattt cccctgttcc ggcagaaacc aggtcaatgc agccccacgg
    22501 ggtgtagcca attaagtcaa caccatcttc aactacggct tttttcattt cccgaatatg
    22561 ggaagcaaag taatcaatgc gatagtgatc gttcaccgtg ccgtcagctt gtctctggtc
    22621 aaccgcacca aatccatttt cgacaataaa cagcggcaac tggaaatgat cccagaacca
    22681 gttgagtgaa taacgtagcc ctgccggatc aatttgccat ccccattcgg atgtatcgat
    22741 ataagggttg ctgaccaaat ctcgtggttc aacataatcc agttgcgggt tatcgggcga
    22801 aaattgtgtc gtaaaagaca tgtagtagct aaagccgata aagtcgacac aaccactggc
    22861 aagaatcgcg ttatcttctg gtgtgatatc gaggttgaat cctttcctgg caaagtaatt
    22921 cagcatatgt tgcggataat atccacgagc atgaacatca gtaaaccagt aacgacgatg
    22981 cattgctttc gtggccatca tcatatcgtt gggtgcacac gtcagaggat agatgggaca
    23041 catggcgatc atacagccaa tgttaaattc aggattgatc gattttccag tctgtaccgc
    23101 cagggcactg gcaactaact cgtaatgtac cgcctggtac attatttgtt cgcgctcatt
    23161 gatatcttcc tctggcgaat acaagatacc ggaattagta aatggacaca ggctttcgct
    23221 gaaattcacc tgattattga tttcgttaaa cgtcatccag tactttactt tttctttata
    23281 gcgcgtgaag accgttgatg cgaagcggat aaaaaagtcg atcagtttac ggtttcgcca
    23341 gccaccatat tttgtcacca gatgataagg catctcaaaa tgtgaaagcg tcaccacagg
    23401 ttccattccc tgcttaaggc attcatcgaa cagatcatca taaaattgta aaccctcttc
    23461 attcggctcc tgttcgtcac cttgcggaaa gattcgtgtc caggcaatgg aagttcgaaa
    23521 gcatttgaat cccatctcgg caaataactg aatatctgtt ttatagcgat gataaaaatc
    23581 aattgcttca tgattgggat aattaagccc gtcgataacg ccttctgtca cttcacgcgg
    23641 caccccgtga gcgccagcag tcatcacatc agcgatgctg atgccttttc ctccttcatt
    23701 ccagccacct tccaactgat gcgcggctac cgcaccgccc cataaaaaac cttttttaaa
    23761 tcctgacata cacaactcct taactaaaat agattaccac cagacttccg cctggatgcc
    23821 ggccataaac tcgtctttgc tgttctcatt aaacttaaaa ttcgataatt cgttatccag
    23881 aatattgata tacgttccat agaagcgaat ttctggtcgt gaacctaaaa tgctttcacc
    23941 cactttcaat gcatgccaga gtgtcgtttt ataagccgat tcatttagtg ttacaccctg
    24001 ctgagttttg ttctgttgct taaaccagcc taattcaagc cccgtctgat tccatgtatt
    24061 ccagatccag gccggtctta ttacagtgcg aatactgtca aaatcactat gagcaccact
    24121 ttcataacta taaacatctt cgccgtgaga atagacaagc gcgttagcca taataatatt
    24181 gtcagtaaga tacatctcgc cctgagagat taaacgccag gcgatcccat tggtatgatc
    24241 atcatagtaa tagcgaccat gcgccatcgt attactggca tctgagaaac tggcaaaact
    24301 gctggcatag gaattattgg caacctgtaa cgtaaattca ttaaacgtat cgcgttgcag
    24361 gttttgccgt aaaacagaag tcagcatcca gctatctttc atttcaaaat aactgttgtc
    24421 attttcgtta tcttgttgtt gatccgtttt attaggtgcg gaatatttag ccattaatga
    24481 taatgtcgca tcatcccata acgggatatt gcgataacga acatccacag aattggtatt
    24541 catctggctg gtacgcgaaa aatcacgaga gtaaacatcg acatcatcac ggcttaagga
    24601 catatcaaac agccctacac caagtgccca gttttcaatc cccacacccg cggcgacatc
    24661 cgtggttaag gttttccagt ccagcatctg gatctcatat tgcgggagtt tatgtttgcc
    24721 gacccagaag tctgcctctg gtgcgaaggg taaaaaacct cgcgttgtca ggtaaatatc
    24781 gctgaactgc atgatatttt cattggcact gtcaccaaac caggcatcgt tatactgctc
    24841 acccacgttg ccatcatagg taacgaccgc atttgccgtt ttaccgtcct gattataaac
    24901 acgctgattt aaggtcaggt caaaccaacc gctcatctca ttaccaaaac gtcccagaga
    24961 acccgctgca taagtttgtg gtgagccgtg attaccagta ccccagcctg agcggaagta
    25021 cccctgatag ctgaacccaa tatcatcctt tatatattta ctgatatctt tcagggtcac
    25081 gctttcgata gtggttttgt cgcctttact ttcaataacg gcaacttgct gcgatccatt
    25141 tatagtatgc gctgcggcag acgagttctg ttcaccgttt tcatttttga tgttatcagc
    25201 gacgggagat gtcgctgata tttcagcaag cgaggctgat ttatatttgt tttccgtgat
    25261 gcttttttgt aaggtcgaaa gtcgggattt atatactccc agttcattct gtgttgcttt
    25321 cagctcttgt ttatttttcg acaattcatt ttcaagcaat tcaaggcgct gctctatggt
    25381 taattttgtt gccatcgcgt tcaatggaaa caatgctgta ataatagaaa aagacaaaag
    25441 gctgacgtta agcgtcttta tattcatgac ttatccttaa gagtataata atttcctcca
    25501 gctatcgcac tggaggaaaa aatcaggcat ggagttcttt tcttaatgaa ataatttccc
    25561 tggctaaatc ctggcataat attgcattca tcaaatgatc ctgtgcatga atcagaatta
    25621 aattaacttt tattttgcca cagccttcat cctgactaat taatgcggtc tgaatcttat
    25681 gtgcctgaag acaggcagat tcagaactgg ccattagctg agttgcgccc tgccagtctt
    25741 tttgcccggc acaacgtatg gcttccatcg cgtcagaacg tgcctgcccc gcgttgacca
    25801 gtaactccat aacaagttct tcatctgcaa acatcacaat ctccttcctg ggtaaatgga
    25861 attatccgtt agttgtcatg ttattgactt tgttggcggc aataacaaaa ggtaaataga
    25921 tcattgtcgc cacacctaaa cataaaaaac caaccagtaa agccagccag ttccccccgg
    25981 ttccgaggaa agcaattaaa ggtccgggcg tagtccaggg cacggcataa gcaaccggtg
    26041 gaataatttc catcgaaaca aagaagtaac caatggcaca gttaacaatg ggaaccataa
    26101 taaacggcac catcatgatg gggttaagta caataggaag gccaaacatt atcggttcat
    26161 tgatattgaa aataccgggg ataaatgaca tttttgccag atcacggaat tccgcacgct
    26221 tagaagcgat aaaaatagcc aacaataacc ccaacgtcat acctgaaccg ccgcagttgg
    26281 cgaatgcatc attaatagaa gtccaggtaa tcggatatgg cgcgccccag gtagtgccat
    26341 gttgagcggc ccaggagaga ttctccagat tagcctcaga aaaaacggtt tctcgaattg
    26401 ccgatgtggt attaggtccg tggatcccca gcacccacag gaaattaccc acagccccca
    26461 ggataattac cgcaaagata ttcgttccca tatgtttgag cggcgtctgg attaatgtgt
    26521 aaataagatc gtttagtcct gccggggata tcagtgccag gcaataatta agtgcggaaa
    26581 agaacaccat gacaaaaaat attggcaata aaactttaaa tgaacgcgct accgcaggcg
    26641 gtacagctgc cggcatcgtt atggtgattt ttgggtttcg agctaagcga caaaatattt
    26701 cactggtgat caatgcaacg ataacagcaa caaacaaccc ctgcgcgccg agatatttgg
    26761 tcgtcaggaa tgcttgccca tctatcatct gatatggcgt ataaacaata aaaaatgctc
    26821 ctattgccgt taatccgcat aaaagatcat cttgctcata gctaatcgcc atattcctgg
    26881 cgaccaggaa agcaatcata attgacatga tatttacaga accattcatt accggagtaa
    26941 aaatagcttg tgctttttca aggttgggga aaatgctatt cagatgcagt aatccggcaa
    27001 taaaaccgtc tggcgataat atggcaaagt taattaatat aattaatgaa cttgccatga
    27061 taattggaaa cgtaaaaacg aatccatctc tcaccgctgc aacatgtttt tgtgagttta
    27121 acttgatagc aactggaaca agaaaacgtt ccattccacg ttcgaatgag gccattaatc
    27181 ccattttttt atacctgttg ttttggatat ttatacatcc caataaagca tgtatgccat
    27241 atccatttta tgatgtgtga aaagttagtt caggtaatag tttaataata ttagattctt
    27301 actcttgcag tagtttcatc gcaaattcaa gaactgcctg tccgttcatg gtgccgtagt
    27361 ctttcatctc gataacggct acaggcattt tcccctgtgc aacggcttca atctctggtt
    27421 tctgaaagcg aacttgcggg ccaagtaaaa caacgtcaac ttcgttattt ttaattttcc
    27481 ctttagcctc tgcaatcgcc aatgcggaaa tattaacttc aagtgaaata gcgttagcat
    27541 gatcaatcat acgtttaacc agcatactgg ttgacatgcc cgcagcgcaa actaacaata
    27601 tttttttcat attttccctt actggtatat aacagattac attgtttaaa tatcatatcc
    27661 cgcagtagct atacgtcctg gtacattgat cacagtaata cagtgataaa gttgttcaac
    27721 atcagggtga ttattaaacg actcggccat acgataaacc aatcaggact caactccttg
    27781 ttttcaatgg ctcttaaaag gtgcaaaagt gatttttcaa aagattgccc gcttacttaa
    27841 atccgaaatc aatggcaatt catggcatgt tggtgatttg ttgccgtcag aagcggaact
    27901 cgctgttcgt tataatgttt cgcgtaatac cctgcgtaag gcattatcct tgctggaagg
    27961 cgaaggaatt attcacagaa agcatggttc aggaacatac attcaaaaaa agaattttgt
    28021 tgcacacatt gatcacatga acagtttcag tgaaattgcg cataaaagcg gcaaagaggc
    28081 agggagtcag attatgaaat ttgaagtgca ggatgcttca cccactattg cgactgagct
    28141 gaatttagtg actggtgagc aggtttatta cataaagaga ctgcgattca ttgaggataa
    28201 tgcagcacaa ttagaagaaa cgtggatgtc agtagcacgt tttcctgatt taaccgtgtc
    28261 acatatgcaa aaatccaaat tttcatatat tgagaatgaa tgcgggatca aaatcattgg
    28321 cacctttgaa actttctccc ctacttttcc taccccagaa atcgccagta ttttacggat
    28381 cagcccacgg gatcccatac ttaaaattca gacccaggct gtggacagta actctattcc
    28441 gctggattat tcgttacttt acagcaatat tttcgagttc caggtaaagt actttttccc
    28501 gcgataaaag cagacgcaca gagtgctgac atcacattct gtgcgtatgg tttagcctct
    28561 aacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc cggtcataat
    28621 gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata gtgccgcact
    28681 tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg tttcatagcg
    28741 tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg ccagatacat
    28801 cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg atgcggaacc
    28861 atcgagaatc agcgaggcaa caaagaaggg aatgattggg attaaagcgc tccagattac
    28921 cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga agatgttgcc
    28981 acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg ccacatgctg
    29041 accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg cgatccccgc
    29101 caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga tagtaaaaaa
    29161 cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt taatggcgca
    29221 gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga gcaaattcag
    29281 tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca taaagcgcaa
    29341 accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca caaaatttag
    29401 cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca ttctaccgcc
    29461 tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct ttaacagttg
    29521 attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat acttcggatt
    29581 tttgccatgc tatttcttta catctctaaa acaaaacata acgaaacgca ctgccggaca
    29641 gacaaatgaa cttatcccta cgacgctcta ccagcgccct tcttgcctcg tcgttgttat
    29701 taaccatcgg acgcggcgct acgctgccat ttatgaccat ttacttgagt cgccagtaca
    29761 gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt ggcgtcgttt
    29821 ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat atgttactgg
    29881 caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac gtgacgctgg
    29941 tggtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc gtgctgaaag
    30001 cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca atcaactaca
    30061 ccatgctcaa cattggctgg accatcggtc cgccgctcgg cacgctgttg gtgatgcaga
    30121 gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg cttttcattc
    30181 aaatttgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt gtctggtcgc
    30241 cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt tttctggctt
    30301 cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg attgctgatg
    30361 gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc atggtggtta
    30421 cgttgcaata ttccgtgggc cgccgactta acccggctaa catccgcgcg ctgatgacag
    30481 caggcaccct ctgtttcgtc atcggtctgg tcggttttat tttttccggc aacagcctgc
    30541 tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat gcgccgggcg
    30601 agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat ttttccgccc
    30661 agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta gtgctaacca
    30721 gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct gcgtgggtgc
    30781 tgatgttaaa agggattcga gcaagaccgt gggggcagcc cgcgctttgt tgatttaagt
    30841 cgaacacaat aaagatttaa ttcagccttc gtttaggtta cctctgctaa tatctttctc
    30901 attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg aggcaaataa
    30961 tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt tctggttttc
    31021 tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc cctcacctaa
    31081 ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg aaaaaaaacc
    31141 gccagtaaac cggcggtgaa tgcttgcatg gatagatttg tgttttgctt ttacgctaac
    31201 aggcattttc ctgcactgat aacagatcgt tgacacagta gcatcagttt tctcaatgaa
    31261 tgttaaacgg agcttaaact cggttaatca cgttttgttc gtcaataaac atgcagcgat
    31321 ttcttccggt ttgcttaccc tcatacattg cccggtccgc tcttccaata accacatcca
    31381 gaggctcttc aggaaatgcg cgactcacac ctgctgtcac ggtaatgttg atatgccctt
    31441 cagaatgtgt gatggcatgg ttatcgacta actggcaaat tctgacacct gcacgacatg
    31501 cttcttcatc attagccgct ttgacaataa tgataaattc ttcgcccccg tagcgataaa
    31561 ccgtttcgta atcacgcgtc caactggcta agtaagttgc cagggtgcgt aatactacat
    31621 cgccgattaa atgcccgtag gtatcattaa ccaatttaaa tcgatcaata tccaacaaca
    31681 ttaaataaag attcagaggc tcagcgttgc gtaactgatg atcaaaggat tcatcaagaa
    31741 cccgacgacc cggcaatccc gtcaaaacat ccatattgct acggatcgtc agcaaataaa
    31801 ttttgtaatc ggttaatgcc gaagtaaaag aaagcaaccc ctcctgaaag gcgtcgaaat
    31861 gcgcgtcctg ccagtgattt tcaacaatag ccagcattaa ttcccgacca cagttatgca
    31921 tatgttgatg ggcagaatcc attagccgaa cgtaaggtaa ttcatcgtta tcgagtggcc
    31981 ccagatgatc aatccaccga ccaaactggc acagtccata agaatggtta tccgttattt
    32041 ctggcttact ggcatctctc gcgaccacgc tgtgaaacat actcaccagc cactggtagt
    32101 gggcatcgat agccttattg agatttaaca agatggcatc aatttccgtt gtcttcttga
    32161 tcattgccac tcctttttca cagttccttg tgcgcgctat tctaacgaga gaaaagcaaa
    32221 attacgtcaa tattttcata gaaatccgaa gttatgagtc atctctgaga taacattgtg
    32281 atttaaaaca aaatcagcgg ataaaaaagt gtttaattct gtaaatcacc tctgcattat
    32341 cgtaaataaa aggatgacaa atagcataac ccaataccct aatggcccag tagttcaggc
    32401 catcaggcta atttattttt atttctgcaa atgagtgacc cgaacgacgg ccggcgcgct
    32461 tttcttatcc agactgccgc taatgttgat catctggtcc ggctgaactt ctcgtccatc
    32521 aaagacggcc gcaggaataa cgacattaat ttcaccgctc ttatcgcgaa aaacgtaacg
    32581 gtcctctcct ttgtgagaaa tcaaattacc gcgtagtgaa accgaagcgc catcgtgcat
    32641 gttttttgcg aaatcaacgg tcattttttt tgcatcatcg gttccgcgat agccatcttc
    32701 tattgcatga ggcggcggtg gcgctgcatc ctgttttaaa ccgccctggt catctgccaa
    32761 cgcataaggc atgacaagaa aacttgctaa tacaatggcc tgaaatttca tactaactcc
    32821 ttaattgcgt ttggtttgac ttattaagtc tggttgctat ttttataatt gccaaataag
    32881 aatactgcca attgttataa ggcatttaaa atcagcccac tagctgtcaa atattcagag
    32941 aatttaac
//
LOCUS       DAZGXZ010000046        32917 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000046 DAZGXZ010000000
VERSION     DAZGXZ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.046"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1819)
                     /gene="selB"
                     /locus_tag="ACHKJF_002258"
     CDS             complement(<1..1819)
                     /gene="selB"
                     /locus_tag="ACHKJF_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP0949609.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
     gene            complement(1816..3207)
                     /gene="selA"
                     /locus_tag="ACHKJF_002259"
     CDS             complement(1816..3207)
                     /gene="selA"
                     /locus_tag="ACHKJF_002259"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP0949610.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(3305..3913)
                     /locus_tag="ACHKJF_002260"
     CDS             complement(3305..3913)
                     /locus_tag="ACHKJF_002260"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP0949611.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(3984..5120)
                     /locus_tag="ACHKJF_002261"
     CDS             complement(3984..5120)
                     /locus_tag="ACHKJF_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0949612.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(5123..5485)
                     /locus_tag="ACHKJF_002262"
     CDS             complement(5123..5485)
                     /locus_tag="ACHKJF_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0949613.1"
                     /translation="MFLNYFALGVLIFVSLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQSERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6022..7935
                     /gene="mtlA"
                     /locus_tag="ACHKJF_002263"
     CDS             6022..7935
                     /gene="mtlA"
                     /locus_tag="ACHKJF_002263"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP0949614.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETMAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGTENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            8030..9178
                     /gene="mtlD"
                     /locus_tag="ACHKJF_002264"
     CDS             8030..9178
                     /gene="mtlD"
                     /locus_tag="ACHKJF_002264"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0949615.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            9178..9765
                     /gene="mtlR"
                     /locus_tag="ACHKJF_002265"
     CDS             9178..9765
                     /gene="mtlR"
                     /locus_tag="ACHKJF_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP0949616.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(9777..9986)
                     /gene="yibT"
                     /locus_tag="ACHKJF_002266"
     CDS             complement(9777..9986)
                     /gene="yibT"
                     /locus_tag="ACHKJF_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP0949617.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            10271..10633
                     /locus_tag="ACHKJF_002267"
     CDS             10271..10633
                     /locus_tag="ACHKJF_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP0949618.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            11177..11860
                     /locus_tag="ACHKJF_002268"
     CDS             11177..11860
                     /locus_tag="ACHKJF_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0949619.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            11904..16754
                     /gene="ehaG"
                     /locus_tag="ACHKJF_002269"
     CDS             11904..16754
                     /gene="ehaG"
                     /locus_tag="ACHKJF_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP0949620.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            17122..18777
                     /gene="lldP"
                     /locus_tag="ACHKJF_002270"
     CDS             17122..18777
                     /gene="lldP"
                     /locus_tag="ACHKJF_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0949621.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            18777..19553
                     /gene="lldR"
                     /locus_tag="ACHKJF_002271"
     CDS             18777..19553
                     /gene="lldR"
                     /locus_tag="ACHKJF_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP0949622.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            19550..20740
                     /gene="lldD"
                     /locus_tag="ACHKJF_002272"
     CDS             19550..20740
                     /gene="lldD"
                     /locus_tag="ACHKJF_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP0949623.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            20926..21399
                     /gene="trmL"
                     /locus_tag="ACHKJF_002273"
     CDS             20926..21399
                     /gene="trmL"
                     /locus_tag="ACHKJF_002273"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP0949624.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(21452..22273)
                     /gene="cysE"
                     /locus_tag="ACHKJF_002274"
     CDS             complement(21452..22273)
                     /gene="cysE"
                     /locus_tag="ACHKJF_002274"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP0949625.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(22353..23372)
                     /gene="gpsA"
                     /locus_tag="ACHKJF_002275"
     CDS             complement(22353..23372)
                     /gene="gpsA"
                     /locus_tag="ACHKJF_002275"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP0949626.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(23372..23839)
                     /gene="secB"
                     /locus_tag="ACHKJF_002276"
     CDS             complement(23372..23839)
                     /gene="secB"
                     /locus_tag="ACHKJF_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP0949627.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(23902..24153)
                     /gene="grxC"
                     /locus_tag="ACHKJF_002277"
     CDS             complement(23902..24153)
                     /gene="grxC"
                     /locus_tag="ACHKJF_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP0949628.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(24295..24726)
                     /locus_tag="ACHKJF_002278"
     CDS             complement(24295..24726)
                     /locus_tag="ACHKJF_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP0949629.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVIDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            24971..26515
                     /gene="gpmM"
                     /locus_tag="ACHKJF_002279"
                     /gene_synonym="pgmI"
     CDS             24971..26515
                     /gene="gpmM"
                     /locus_tag="ACHKJF_002279"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP0949630.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            26549..27808
                     /gene="envC"
                     /locus_tag="ACHKJF_002280"
     CDS             26549..27808
                     /gene="envC"
                     /locus_tag="ACHKJF_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP0949631.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            27812..28771
                     /locus_tag="ACHKJF_002281"
     CDS             27812..28771
                     /locus_tag="ACHKJF_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP0949632.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(28758..29792)
                     /gene="waaH"
                     /locus_tag="ACHKJF_002282"
     CDS             complement(28758..29792)
                     /gene="waaH"
                     /locus_tag="ACHKJF_002282"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0949633.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(30031..31056)
                     /gene="tdh"
                     /locus_tag="ACHKJF_002283"
     CDS             complement(30031..31056)
                     /gene="tdh"
                     /locus_tag="ACHKJF_002283"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP0949634.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(31066..32262)
                     /gene="kbl"
                     /locus_tag="ACHKJF_002284"
     CDS             complement(31066..32262)
                     /gene="kbl"
                     /locus_tag="ACHKJF_002284"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP0949635.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(32537..>32917)
                     /locus_tag="ACHKJF_002285"
     CDS             complement(32537..>32917)
                     /locus_tag="ACHKJF_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFP0949636.1"
                     /translation="KIWDFPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGA
                     IVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFD
                     LFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         8257 a         8617 c         8357 g         7686 t
ORIGIN      
        1 cgcgcaataa atgatcattt ccacgacgac gcgtaaagcc aatgcggtca aaatattcca
       61 gaatctgaat tgccagcttt cggcctacgc ctaagcgatc gcggaaatcc gccgcgcagg
      121 ttgaaccaca ctcctgatcg agatcgcgga tcatattggc aaattcgaca atccgatcgt
      181 tacggtaata acgatctttg acgatcgcgg taattattcc ttgctgcgcc gcctggcgta
      241 gcgtcaggcg cattgcctgc tcgtcggtac ccgtctcttt tgccagatca cgcacccacc
      301 acggctcgtc accaaacagc ggctctgctt tttgccagat ggcctgctgc tcttcgctga
      361 agcccgcttt gtgatccggc agatgcagcc aaccgtgatg actgaggatg tcgccgcttt
      421 cgcgcatctt ttcaatcagc aacagtacta gcgcttcatc ttccattggc aacgccatac
      481 gtcgcagacg ttcgcgccca ggcccaggtt catcgcgatg ttgctcatga taagtcgcta
      541 atgtgtcgag aattttccgt tgccagcgag cggcaaccgg cgcattcaac aagctataac
      601 cagcctgaat atagccaggc tgttgcagca attcgcgcat cccttcgccg ttgagctggc
      661 gcgcccaggc gaaatccgca aggttaaccg cgccgcgttc cagatgaaca gataacgcat
      721 cggcatcgct ctgcgcccgt gcaagagacg ccagccattg cagatattcc ggcttacgtt
      781 taccgcgacg cggcgggtta agcatcacca cacgcgcccc cgccagcgtg ttgcgggccg
      841 agatatcgcg cagcactaag cgatcgttat ccgccagcca cagcggcgtg tcgaacacca
      901 gctcggcaag gttatcttcc agcagcgaaa cgcgtcccgt aacgtggctg gcggcgtggt
      961 gaatatgcag cggctgccac tgggtcagcg gtgtatgggt ttgaagctcg acaatcaccc
     1021 gtgtgaacgg ctctggcggc acatcggcaa gcagccagtc gccacggtta atctgctctt
     1081 tttccgcatc acccgcgatg ttaagcgcga tacgctgccc ggcatgggcg gtttctgttg
     1141 gctggttttg cgcatgcagc gcacgtacac gcatcggttt atttacacca gtcagccaga
     1201 gtgaatcgcc taccttcact tccccgctta acgccgtacc ggtgacgacc agcccggcac
     1261 cttttacggt aaatgcgcgg tcaatagcga ggcggaaact atgttggctg gcgtgctcgc
     1321 gttccggcaa ctgaagcaga tgctcgcgca gggcatcaat tccccgacct tcggttgctg
     1381 cggtgataaa cagttttgcc tcagcaaaac cgtattcccg cagcacctcc tttacctggc
     1441 gttcaacctc attaacacgc gcttcgtcca cgcgatcggc tttggtcagc gccactgtca
     1501 gcatcgggtt accggtcagc tgcaaaatcg ccagatgctc acgggtctgt gccatcacgc
     1561 cgtcatcgca cgccaccacc aacagcgcgt gatcgatacc accaacgccc gccagcatgt
     1621 tggaaagaaa cttttcatga ccgggtacgt cgataaaacc aggcacgcga ccatccggct
     1681 gcggccagta ggcatagccg agatcgatgg tcatgccgcg ctttttttct tccggcagac
     1741 ggtcagcatt tacgccagta atcgcctgca ataaggttgt tttgccgtgg tcaacgtgtc
     1801 cggcagtcgc aataatcatt tcaacaacat ctccaaaaac cgttgctcat cttcaaggca
     1861 gcgtaaatcc agccacaatc gtccgtcata aatacgacca atcaccggca ctggcaattc
     1921 acgccagcgg gcggctaatg actcaaggtg gctaccgcgt ccatcatggg gtgtaaacgt
     1981 taatgccgcg ctcggcaggc gatcaaccgg cagcgaacca ctgccaatct gcgaaagaca
     2041 tggcataacc tgtaccgcaa actccgcgcc gtaatgtgcg gcaagggggg cctgtaaacg
     2101 ttgtgcctgg atttgaatga cctctgcgct gcgggtaagc aggcgcaggg tcggtaattt
     2161 ttcactcaga gcttcagggt gtaaataaag acgcaacgtg gcttccagcg ccgcgagggt
     2221 cattttatcc gcgcgtaatg cacgcttcag cgggtggctt tgcaggcggg cgatcatctc
     2281 ttttttacca acaataattc ctgcctgcgg cccgcctaac aacttgtcgc cggagaaact
     2341 caccagactg acgcccgccg caatcaactc ctgcggcatt ggctctttcg gcaaaccgta
     2401 ctggctaaga tcgaccagcg agccactgcc taaatcagtc actacgggaa catccagctc
     2461 tttgccgagc gccaccagtt ccgcttcatc tatcgctttg gtgaacccct gaatgctgta
     2521 gttactggta tgtactttca tcaacagtgc ggtattttca ttcaccgcct gacgataatc
     2581 attcgcgtgc gtgcggttgg tggtccctac ttcgtgtagg gtgcagcctg cctgacgcat
     2641 aacatcggga atacgaaacg cgccgccaat ctccaccagt tcgccgcgag aaaccaccac
     2701 ctctttcccg ctggcagtgg ccgccaacat caataacacc gccgccgcat tgttattaac
     2761 gatacaggca tcttccgccc ccgtaatgcg gcacagcaac tgcgccagcg cacgatcgcg
     2821 atgtccgcgt ccagcgtcgt ccagatcgta ctcgagggtc actggcgaac gcatagcctg
     2881 cgcaacggct tccaccgcgg cttccgcctg taaagctcgc ccaaggttgg tatgcagcac
     2941 ggttcccgtc aggttgatca ccggacgcag cgcgctctgc gcttctttcg tcaaccgggc
     3001 atcgacttct tgcgcccagt tttcacacca cgcaggcagc gtctggctgc cacgaatcac
     3061 ttctcgcgct tcgtcgagca tctgacgcaa caattccacc acgcgggtgt gaccataagt
     3121 atcacgcaaa gaaaggaagg agctatcgcg caataagcga tcaatagccg gaagttgact
     3181 atagagggaa cgcgtttcgg ttgtcatagg aaacctggct gatcaaggcc ctctcacacg
     3241 gagaagggcg tttaacataa ccacggattg taacgtgaga tgggtcagga ggacatatcg
     3301 cgcatcaagc ctttggcggt tcggtgcgag caaaactttc gcggctaaac aggttttcga
     3361 ccagtttgac taagtgtggg cgatcgacac accagcctgg cgcaacgcgg cggaaattga
     3421 gatatccgac ggcacaggca atggcgatag tcgccagatt gaccgtatcg gttttgagtg
     3481 tgccatcgac cagatatcct tccagcacat ccagactgcg gttgattttc tcccgctggc
     3541 gtaacaattc atcttcagac tgctgcgccg ctggacgcgc ctgttcacgc accgatacca
     3601 gcccggcatc cataatgcca tccgccagtg cctcaatttt gcgcacccgc aacgactcca
     3661 gcggatcgcg cggcaacatc gccggagcga cattcattaa ttcaatgtat tcagcgatga
     3721 tcggcgaatc aaaccagcat tcgccctctt cggtcaccag caccggcact tttcctaacg
     3781 ggttaaattg cgccacgccg ttgtccgcgt tatagggcag ttcattaata aattcgaaag
     3841 ttatgccctt ttctaacaac agaatagaaa gtttgcgtac aaacgggctg gtgtagctac
     3901 cgacgagttt catgccgagt cctttgtgcg aggaaaaata tcagtatggc tcaggctcac
     3961 gcaaaacaca ggtgatataa gtctcaatga tccaaataaa gataatgcat ccagctggtc
     4021 attcttagca gcactttccg catcaccgaa acatggctga aatggtcgga gtaaactgcc
     4081 acctgggcgt agatgccgtc gggtaaggca tcgatatcat cgttaggatc cagttcaatg
     4141 gttcccagca caccgtccgt gccgggcacg accgttaatg attgcaatac cccctgcgcc
     4201 tgataagaac cgcctggcac gacaggtaaa atactggtca gtttgccgtg gaacacctgc
     4261 ccaggtagcg cgttaaacac cacttccgca tcatcgccag gtttcagacg taacagcgag
     4321 ttttgccgaa attgggcgac aatttgtcgt ttttgctcgg ggatgaaaac catcaccgga
     4381 cgcaacggca aggcagctgc gtatgtacct gggcggatca gtacctgagt aacgtagcca
     4441 ttgctcggcg cgcgaatgac agtctgctca aggttatatt ttgcttcagt aagttgcgct
     4501 cttaagctca caatctgcga ttgctcgccg ttaaccatac tgtcgagctg gctctggatc
     4561 tgcgcctgct ccgccaccga gcctttcacc agcgcatcct gcgcaaggaa attttgccgc
     4621 gcatcgtcga tgtcacgttc cgagaacgga ttcaccgccg cctggctgcc tttcaggtaa
     4681 cgttgataat ttttaaacag acggtcgcgc tccgctgaaa cctgggtggt gttggcctgc
     4741 gcttcagtga gctgcgcgcg cagcgtcttt atattatgcg tcgccgtcat caggtcagcc
     4801 tgaagtctgt caactcgcgc ctggtaacga accgggtcga gcttaaaaag cacctcgccc
     4861 ttttgaataa gctgattatt cttgtcagtg acttcagtaa caattcccgt cacctgtggc
     4921 gtgataggga tcgctatcac tgccttttgc gcggtaaaag tgtaagggtg gttgtagttc
     4981 atcaacaaaa tcaaaccact caccagaaac acgcctccca gcgccgccgt cgccagcgtc
     5041 cactgattta ccggaatgcg gaagatttta aagaccgccc acgccagcgc cacgtaagtt
     5101 aaaacaatca atagatccat aattagatct ccgggaacgt ggaatagtca gtctttttct
     5161 cggcggcgag ttgatgctcc agccgggaaa ttcggtcaga aagtgaggcg atttccgggt
     5221 cagtcgcttt ctcctgcgac gcaacatgtg actgcatacc ccagccacgc tccgattgat
     5281 agagcgtcgc ccagatccac agaaacggcc agataacatg cagagtaaac aggctcaccc
     5341 agcccgccgt atgaatagcg tcggcatggg gatggttgcg ctttttggca atcagataag
     5401 ggatgtcatg tatcgcgatg attccataaa aaatcaccag agagacgaag atcagcactc
     5461 ccaacgcgaa atagtttaga aacatatttg cctcggattc acgtttatca gtgttgtttt
     5521 ttggctggca gccagaaggg agtcaggctg atattttgac aataatccgg gttcgcgatt
     5581 ctcgccataa caccaaagaa taatttttag aggtgatgag ttgcttagtt acataacgat
     5641 tgtatgacga aggcataaca tgctgtagat cacatcaggt gaacgccgta agaaaatatc
     5701 ttgtgattca gatcacaaag attcaacaaa ccatcaaaac aaaaatgtga cattactcac
     5761 acttaaatgc catttttagc gaaaatcgcc gtcttgttgc ttttttacac aagcgttttg
     5821 tgatgagtat cacgtcaatt acctctctac cccctatatt tatgtgactg atatcacaca
     5881 aaaggccgtc gactggacag ttaaccgatt cagtgccaga tttcgcagta tctacaaggt
     5941 ccggctacct ctgccgccac attaacaaaa aacctcgggc ttccagcctg cgcgacagca
     6001 aacataagaa ggggtgtttt tatgtcatcc gatattaaga tcaaagtgca aagctttggt
     6061 cgtttcctca gcaacatggt gatgccaaat atcggcgcgt ttatcgcgtg gggtatcatc
     6121 accgcgttat ttattccaac agggtggtta ccgaacgaga cgatggcgaa gctggtcggg
     6181 ccgatgatca cttatctcct gccgctgctg atcggttata ccggtggtaa gctggtaggc
     6241 ggcgaacgtg gcggcgtagt cggtgccatc accaccatgg gcgttatcgt cggcgcagat
     6301 atgccgatgt tcctcggttc gatgattgca ggtccgctgg gcggctggtg cattaagcac
     6361 ttcgaccgct gggtagacgg taagatcaaa tccggttttg agatgctggt gaataacttc
     6421 tccgcaggca tcatcgggat gatcctcgcc attctggcat ttctcggcat tggcccgatt
     6481 gttgaagccc tgtccaaaat gctggctgcg ggcgttaact tcatggttgt ccatgacatg
     6541 ctgccgctgg cgtctatctt tgttgaaccg gcgaaaatcc tgttcctcaa caacgccatt
     6601 aaccacggta tcttctcgcc gctgggtatt cagcagtccc atgaactggg taaatccatc
     6661 ttcttcctga ttgaagctaa cccaggtccg ggtatgggcg tgctgctggc gtacatgttc
     6721 tttggtcgtg gtagcgctaa acagtctgcg ggcggtgcgg caatcatcca cttcctgggt
     6781 ggtatccacg aaatttactt cccgtatgtg ctgatgaatc cgcgtctgat cctggccgtt
     6841 atcctcggcg gtatgactgg cgtgttcacg ctgactatcc tgggcggtgg tctggtttct
     6901 ccggcatctc cgggttctat ccttgctgta ctggcgatga caccaaaagg tgcttatttc
     6961 gctaacatcg cgggtgtgtg tgcggcgatg gcagtctcct tcgttgtctc tgctattttg
     7021 ctgaaaacca gcaaagtgaa agaagaagat gatattgaag cagcaactcg tcgtatgcag
     7081 gacatgaaag ctgagtctaa aggtgcatct ccgctgtctg cgggcgatgt aactaacgac
     7141 ctgagccacg tacgtaaaat catcgttgcc tgtgacgccg gtatggggtc cagtgcgatg
     7201 ggcgcaggcg tgctgcgtaa gaaaattcag gatgcaggtc tgtcgcagat ttcggttact
     7261 aacagcgcga tcaacaacct gccaccagat gtggacctgg tcatcactca ccgtgacctg
     7321 accgaacgcg ctatgcgcca ggttccgcag gcacagcata tttcgctgac caacttcctc
     7381 gacagcggcc tgtacaccag cctgaccgaa cgtctggttg ctgcccaacg ccacacggca
     7441 aacgaagaga aagtaaaaga cagcctgaaa gacagctttg acgattccag tgctaacctg
     7501 ttcaagctag gcacggagaa catcttcctc ggtcgcaaag cggccaccaa agaagaagcg
     7561 attcgttttg ctggcgagca gctggtgaaa ggcggttacg ttgaaccgga gtacgttcag
     7621 gcgatgctgg atcgtgaaaa actgaccccg acttatctgg gtgagtctat cgcggtgcca
     7681 cacggtacgg ttgaagcgaa agatcgcgta ctgaaaacgg gcgtggtgtt ctgccagtac
     7741 ccggaaggcg tgcgcttcgg tgaagaagaa gatgacattg cccgtctggt gattggtatt
     7801 gctgcccgta acaacgagca cattcaggtt atcaccagcc tgaccaatgc actggatgat
     7861 gagtccgtca tcgagcgtct ggcacacacc accagcgtgg atgaagtgct ggaactgctg
     7921 gcaggtcgta agtaatccaa tcctaccctc tcctcacgga gagggtttgg gtgagggaaa
     7981 agcctcaccc cagccctctc gggtaaaaac attgatgaag gttaatacta tgaaagcatt
     8041 acattttggc gcaggtaata tcggtcgtgg ctttatcggt aaactgctgg cagacgcggg
     8101 tatccaactg acgtttgccg atgtcaatca ggtggtactt gatgccctga atgcccgtca
     8161 tagctatcag gttcatgtgg tcggtgaaac cgagcaggta gataccgttt ccggcgtcga
     8221 tgctgtcagc agcattggtg atgatgtcgt tgatctgatt gctcaggttg atttagtcac
     8281 taccgccgtt ggcccggttg tgctggaacg tattgctccg gctatcgcca aagggctggt
     8341 gaaacgtaaa gaacaaggta atgaatcccc gctgaacatc atcgcctgtg aaaacatggt
     8401 acgcggtacc acgcagctga aaggccatgt gatgaacgcc ctgccggaag acgccaaagc
     8461 gtgggtagaa gaacacgttg gctttgtcga ttccgccgtt gaccgcatcg taccgccttc
     8521 ggcttcggca actaacgatc cgctggaagt gacggtagaa actttcagcg aatggattgt
     8581 cgataaaacg cagttcaaag gcgcactgcc gaacatccca ggcatggagt taaccgacaa
     8641 cctgatggca tttgtcgaac gtaaactctt caccctgaac acgggtcatg ctataaccgc
     8701 gtacctcgga aaactggccg gtcatcagac cattcgtgac gctattctcg acgagaaaat
     8761 ccgcgcggtg gtaaaaggtg cgatggaaga aagtggtgcg gtactgatca agcgctacgg
     8821 ctttgacgca gacaaacatg cggcgtacat ccagaaaatc ctcggtcgtt ttgagaaccc
     8881 gtatctgaaa gatgatgtag agcgcgtagg ccgtcagccg ctgcgtaaac tgagtgctgg
     8941 cgaccgtctg atcaagccac tgctcggtac gctggaatac agccttccgc acaagaatct
     9001 gattcagggg attgctggtg caatgcactt ccgcagtgaa gatgatccgc aggctcagga
     9061 actggcagca ctgatcgctg acaaaggtcc gcaggcggcg ctggcacaga tttccggtct
     9121 tgatgccaac agcgaggttg tatccgaggc ggtaaccgct tataaagcaa tgcaataatg
     9181 gtggaccagg cgcaggacac cctgcgcccg aataacagat tgtcagatat gcaggcaaca
     9241 atggaacaaa cccaggcctt tgaaaaccgt gtgcttgagc gtctgaatgc tggcaaaacc
     9301 gtgcgaagct ttctgatcac cgccgtcgag cttttgaccg aggcggtaaa tcttctggtg
     9361 cttcaggtat tccgcaaaga cgattacgcg gtgaagtatg ctgtagaacc gttactcgac
     9421 ggcgacggtc cgctgggcga tctttctgtg cgtttaaaac tcatttacgg gttgggcgtc
     9481 attaaccgcc aggaatacga agatgcggaa ctgctgatgg cattgcgtga agaactaaat
     9541 cacgacggca acgagtacgc ctttaccgac gacgaaatac ttggaccctt tggtgaactg
     9601 cactgcgtgg cggcgttacc accgccgcca cagtttgaac cagcagactc cagtttgtat
     9661 gcaatgcaaa ttcagcgcta tcaacaggct gtgcgatcaa caatggtcct ttcactgact
     9721 gagctgattt ccagaatcag cttaaaaaaa gcctttcaaa agtaagcaac gtctgcttac
     9781 tgcccctcta cctgcttcgg ccgataaagc cgacgataat actccagacg ttgtagatat
     9841 aacggcacgc tttcatcggg tattccggac ggaatcgcgt tacggggagg aagttttttc
     9901 agatactccc ggaacgcctg gcttgatgcc atgaaatcta cggctttatc gataagaagc
     9961 ggaacgtttt cacctaattt gcccatgatg atatctccgt attaccccgc gccgggaatg
    10021 cgcggccgcc aattttagtt taggttcccg gtaaagatga cattaaggaa aacgtgctga
    10081 atcctcaata atcatccgct gctgtttatt atcttttttc tatagatttc ttaattaatc
    10141 aataaattgc gtagttttta agcatgttaa tgattacgtt acagaactat tgcaatgtca
    10201 tcagttgtga acaaagcacc tggtcgcgca tactaggggc tataaattta tctttatcag
    10261 aagccatcac atgaaagaag tcgaaaaaaa cgaaatcaaa cgtctcagcg atcgtctgga
    10321 cgccatccgc caccagcagg ccgatctgtc gctggttgaa gccgcagaca aatatgccga
    10381 gctggaaaaa gagaaagcca cgctggaagc agaaattgct cgcctgcgtg aagttcatag
    10441 ccaaaaactg agtaaagaag cacagaagct gatgaagatg ccgtttcagc gcgcaattac
    10501 caaaaaagag caggctgata tgggcaagct gaagaaaagt gttcgcggac tggtggttgt
    10561 gcacccaatg accgcgctgg gccgtgaaat gggcctgcag gagatgactg gattctctaa
    10621 gactgcgttt taagcgcctc agacgactgg ttcgatcata tcaacccgca tctggcttgc
    10681 cttagctggt caatgcgggg actctcctgt ctactcgcct aattaccaaa caattcactc
    10741 cctttacttc gatttaccgc tggttgatat tctgaaaatg catttttacc gaaatagcca
    10801 tctataaaca ataaaaatat atagaagata tcatttgtta attaacaagc atttacgtct
    10861 ctttttttcg gatttaattg tgaaatttta atcatcatat tcacaaaatc aattatctgt
    10921 aaaatttttc ccagacatgg tttctttcat atttgatatt tattaataat catcattaga
    10981 atccacgaaa aataatatat tcatacaaac acagacatct tttgaaacga atgatacatt
    11041 tttatgatga atgcttaaaa caatatttaa tcgacacaca tcactgtcat taatgtaaag
    11101 caacatagct caccaataat acaatggaca actctaccgg cattgatcgc tggtcggagg
    11161 aaataaggat ttttacatga acttgaagaa gatttttttc agtgctgtca ctgtttcagt
    11221 tctctgcgca ttaacgggat gtgactacat tgaagaaggc aagccagaaa gtagtttact
    11281 gaaacaacaa gaagaacata ataataaaat tgtgctactt gaaaaacaac aagcacagct
    11341 aaagagtcaa ctcgaaacta ttcaaaaaca acaaactggc ataataaaca gtacaaagac
    11401 tttaactcac gtaattaaat ccgttaaaga ccaacaaaat acttttattt ttaccgagtt
    11461 taacccagca aaaacgaagt atttcatcct gaacaacggt tcggtggctt tagcgggtcg
    11521 agtgttatcc attgacgcca cagagaatgg tagtgttatt catatctcac tggtcaactt
    11581 attaagtaca cctatctcaa acattggttt taatgcgaca tggggtggtg aaaaacctgt
    11641 cgatgccaaa gagtttgccc ggtggcaaca attacttttc aacacatcaa tgaaatccac
    11701 attgaaatta ttaccaggtc aatggcaaga cattaatttg accctgaagg gtgtatcgcc
    11761 caataacctg ggatatctga agttagccat caacatggaa aatattcagt tcgacaatct
    11821 tccctctgct gaaaatcggc agaaaagaag caaaaaataa atgcagttag tacgcttata
    11881 acaaggaata gcgagcatca ttaatgaaca aaatatttaa agttatctgg aaccctgcga
    11941 cagggaatta tactgttacc agcgaaacgg caaaaagccg tggcaagaaa tctgggcgca
    12001 gtaagctgtt aatttctgcg ctggttgcgg gtggaatgtt gtcgtcgttt ggggcattgg
    12061 cgaatgccgg gaatgacaac ggtcagggtg ttgattacgg tagtggatca gctggcgacg
    12121 gctgggttgc tataggcaaa ggggcgaaag caaatacttt tatgaacacc agtggttcca
    12181 gtactgctgt gggttatgac gctatagctg aaggccaata tagctctgcc atcgggtcaa
    12241 aaacccatgc gattggcggt gcatcaatgg cctttggggt tagtgcaata tcagaaggcg
    12301 atagaagtat agcactgggt gcctcttcgt attcattggg ccaatactca atggccctcg
    12361 gccgttattc aaaagcattg ggtaaattgt ctattgctat gggggactct tccaaagcgg
    12421 aaggagcaaa cgccattgcc ctgggaaatg ccactaaagc tactgagatt atgagtattg
    12481 ctcttggcga caccgccaat gcgtcaaaag cgtattcaat ggcgctggga gcaagtagcg
    12541 tcgcatctga agaaaacgca attgccctgg ggcgtagcag tgtagctagc ggtactgaca
    12601 gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa cgctattgcg ataggtgctg
    12661 agaccgaagc cgctgaaaat gcaactgcta ttggcaataa tgcgaaggca aaagggacta
    12721 atagcatggc aatggggttc ggaagccttg ccgataaagt caatactatc gcattaggaa
    12781 atggcagcca ggctctggca gataatgcaa tcgccatagg ccagggcaac aaagctgatg
    12841 gcgtggatgc catcgctctg ggtaatggta gccagtcgag aggcttaaac accattgcct
    12901 taggcacagc cagtaatgca actggtgata agagtcttgc gcttggtagt aatagcagtg
    12961 ccaacggtat taactctgtc gcgctgggcg cagattccat tgcggattta gacaataccg
    13021 tctctgtcgg caatagttca ttaaaacgca agatcgttaa tgtgaaaaat ggcgcgatca
    13081 agtctgacag ttacgatgcc attaatggtt cacagcttta tgccattagc gactcggtag
    13141 caaaaaggct tggaggaggg gctgcagtag atgttgatga cggtactgtt acagcaccaa
    13201 cctacaattt aaaaaatggt agcaaaaata acgtaggggc tgcgctcgct gtacttgatg
    13261 aaaacaccct gcaatgggac caaaccaaag gcaaatacag cgctgctcat ggtactagta
    13321 gcccaactgc cagcgtaatc accgatgttg cggatggcac gatttcagcc tccagtaagg
    13381 atgcggttaa cggttcccaa ctgaaagcta ccaatgacga tgtcgaagcc aacaccgcca
    13441 atatcgctac taataccagc aacattgcca cgaatacggc aaatattgcc accaatacca
    13501 ccaatatcac caacctgacg gattccgttg gtgaccttca ggctgatgcc ctgctctgga
    13561 acgaaactaa aaaggcattc agtgcagctc acggccagga taccaccagc aaaatcacca
    13621 acgttaaaga tgccgacctg acggctgaca gcactgatgc tgttaacggc tctcagctga
    13681 aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat cgccaataac acttccaata
    13741 ttgccactaa caccaccaac atctctaacc tgactgagac ggtgactaat cttggtgagg
    13801 atgcgctgaa atgggataag gacaatggtg tattcacggc agctcatggc accgagacca
    13861 ccagcaaaat caccaacgtt aaagatggcg acctgacgac tggcagcacc gatgccgtta
    13921 acggctctca gctgaaaacc accaacgatg ccgtggcgac gaataccacc aatatcgcca
    13981 ctaacaccac caacatctct aatctgactg agacggtgac taatcttggt gaggatgcgc
    14041 tgaaatggga taaggacaat ggtgtcttca ctgcagctca tggcaacaat accgccagca
    14101 aaatcaccaa tatcctggac ggcacagtca ctgcaaccag ttccgatgcc attaacggta
    14161 gccagcttta tgacttaagc agcaatatcg ccacctactt cggcggcaat gcttctgtga
    14221 atactgacgg tgtgtttacc ggtccaacct acaaaatcgg tgaaacaaat tattataacg
    14281 tcggcgatgc actggctgcg attaactcct catttagcac gtctctcggc gatgctctgc
    14341 tttgggatgc caccgcaggt aaattcagtg ccaaacacgg tactaatggt gacgcaagcg
    14401 tgatcactga tgtcgcagat ggtgaaattt cagactccag ttctgacgca gtaaacggct
    14461 cacaactcca cggcgtgagc agttatgttg ttgatgcgct ggggggtggt gccgaagtca
    14521 atgcagacgg caccatcact gcgccgacgt acaccattgc taatgctgat tacgataatg
    14581 tcggtgatgc cctgaatgct atcgatacca ctcttgacga cgctctgctc tgggatgcgg
    14641 acgccggtga aaatggtgca tttagcgccg ctcacggaaa agataaaact gccagtgtaa
    14701 tcactaacgt cgctaacggt gcaatctctg ctgccagcag cgacgcgatt aacggctcac
    14761 aactctatac caccaataag tacatcgctg atgcgctggg tggtgacgca gaagtcaacg
    14821 ctgacggcac catcaccgca ccgacttaca ccattgcgaa cgccgagtac aacaacgtcg
    14881 gtgacgccct ggatgcgctt gatgataacg ccctgctgtg ggatgagact gccaatggcg
    14941 gtgctggagc ctacaatgcc agccatgacg gtaaagccag catcatcact aatgtcgcta
    15001 atggcagtat tagtgaggac agtaccgatg cagtgaacgg ttctcagttg aatgcgacga
    15061 atatgatgat tgagcagaac acccaaatta tcaatcagct cgctggtaac accgacgcaa
    15121 cctatatcca agaaaacggt gcgggtatta actatgtgcg tactaacgac gacggcttag
    15181 cgttcaacga cgccagcgca cagggtgttg gcgctacagc tataggttat aactctgtcg
    15241 ccaaaggcga tagcagcgta gctattggtc agggcagcta cagcgacgtt gatacgggta
    15301 tcgccctggg tagcagctct gtttccagcc gagtgattgc caaaggctcc cgtgacacca
    15361 gcataacgga aaatggcgtt gttattggtt acgacaccac ggatggcgaa ctgctcggtg
    15421 cattgtctat cggtgatgac ggtaaatatc gtcaaatcat caacgtagcc gatggttccg
    15481 aagcccatga cgccgttacg gttcgtcaat tgcagaatgc gattggtgcg gtcgcaacca
    15541 cgccgactaa atacttccac gctaattcaa cggaagaaga ttcactggca gtgggaactg
    15601 actcgctggc aatgggtgcg aaaaccatcg tgaatggcga taaaggtatt ggtatcggtt
    15661 atggtgccta cgtggacgcg aatgcactta acggcattgc cattggtagc aatgcgcaag
    15721 tcattcatgt caacagtatt gcgataggta atggttctac gaccactcgt ggcgctcaaa
    15781 ccaattatac cgcctacaac atggacgcac cgcagaactc tgtcggtgaa ttctcagtcg
    15841 gtagtgcgga tggtcaacgt cagatcacta acgtcgcagc aggttcggct gataccgatg
    15901 cggtcaacgt gggtcagttg aaagtaacgg atgcgcaggt ttcccagaat acccagagca
    15961 ttactaacct ggataatcgg gtaacgaatc ttgattcacg cgtcaccaat atcgaaaacg
    16021 gtattggcga tatcgtcacc accggtagca ccaagtactt caagaccaat accgatggtg
    16081 tagatgccag cgcgcagggt aaagatagcg tcgcgattgg ttctggctcc attgctgccg
    16141 cggataacag cgtcgcactg ggtacagggt ctgtggcaac cgaagaaaat acgatctctg
    16201 taggctcatc tactaatcaa cgtcgtatca ccaacgtagc cgcaggtaaa aacgacaccg
    16261 atgctgttaa cgtggcacag ttgaagtctt ccgaagctgg cggtgtgcgt tacgacacca
    16321 aagctgatgg ttctatcgac tatagcaata tcaccctcgg tggcggcaac ggcggtacga
    16381 ctcgtatcag caacgtctcc gctggcgtca acaacaacga cgcggtgaat tacgcgcagt
    16441 tgaagcaaag cgtgcaggaa acgaagcaat acaccgatca gcgaatggtt gagatggata
    16501 acaaactgtc taaaactgaa agcaagttga gcggtggtat cgcttctgca atggcaatga
    16561 ccggtctgcc gcaggcttat acaccgggtg ccagcatggc ttctattggt ggcggtactt
    16621 acaacggtga atcggcagtt gctttaggtg tatcgatggt gagcgccaat ggtcgttggg
    16681 tctacaaatt acaaggtagt accaatagcc agggtgaata ctccgccgca ctcggtgccg
    16741 gtattcagtg gtaatgatcc attaagttag tgtgactaag ggcaaacgcc aaattgcctg
    16801 atgcgttacg cgcatcaggc aagataaggc gctccgggtc aacaatctaa ggacaattct
    16861 ctgatgagga ttgccctttt ctttaccaga catctccccc cacaagaatt ggccctacca
    16921 attcttcgct tatctgacct ctggttcaca attccccaat taaaactcac atcaatattg
    16981 ccaatacata acatttagtt aaccattcat tgtcattatc cctacacaac acaattggca
    17041 gtgccacttt tacacaacgt gtgacaagga gatgagcaac agactcatta cacgatgtgc
    17101 gtggactcca ggagacctgc aatgaatctc tggcaacaaa actacgatcc cgccgggaat
    17161 atctggcttt ccagtctgat agcatcgctt cccatcctgt ttttcttctt tgcgctgatt
    17221 aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg tagcaatcgc ccttgccgtg
    17281 gctttgctgt tctataaaat gccggtcgct aacgcgctgg cctcggtggt ttatggcttc
    17341 ttctatggtt tgtggcccat cgcgtggatc attattgcag cagtgttcgt ctataagatc
    17401 tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta ttctttcgat aacccctgac
    17461 cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg ctttccttga aggagccgca
    17521 ggctttggcg caccggtagc aattaccgcc gcattgcttg tcggcctggg ttttaaaccg
    17581 ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc cagtggcatt tggcgcgatg
    17641 ggcattccaa tcctggttgc cggacaagta acaggcatcg acagctttga gattggccag
    17701 atggtggggc ggcagctacc gtttatgacc attatcgtgc tgttctggat catggcgatt
    17761 atggacggct ggcgcggtat caaggagacg tggcctgcgg tcgtggttgc gggcggctcg
    17821 tttgccatcg ctcagtacct tagctctaac ttcattgggc cggagctgcc ggacattatc
    17881 tcttcgctgg tatcactgct ctgcctgacg ctgttcctca aacgctggca gccagtgcgc
    17941 gtattccgtt ttggtgattt gggggcgtca caggttgata tgacgctggc ccacaccggt
    18001 tacactgcgg gtcaggtgtt acgtgcctgg acaccgttcc tgttcctgac agctaccgta
    18061 acactgtgga gtatcccgcc gtttaaagcc ctgttcgcat cgggtggtgc gctgtatgag
    18121 tgggtgatca acattccggt gccgtacctc gataaactgg ttgcccgtat gccaccagtg
    18181 gtcagcgagg ctacagccta tgccgccgtg tttaagtttg actggttctc tgccaccggc
    18241 accgccattc tgtttgctgc actgctctcg attgtctggc tgaagatgaa accgtctgac
    18301 gctatcagca ccttcggcag cacgctgaaa gaactggctc tacccatcta ctccatcggc
    18361 atggtgctgg cgttcgcctt tatctcgaac tattccggac tgtcatcaac actggcactg
    18421 gcactggcgc acaccggtca tgcattcacc ttcttctcgc cgttcctcgg ctggctgggg
    18481 gtattcctga ccgggtcgga tacctcatct aacgccctgt tcgccgcgct gcaagccacc
    18541 gcagcacaac aaattggcgt ctctgatctg ttgctggttg ccgccaatac caccggtggc
    18601 gtcaccggta agatgatctc cccgcaatct atcgctatcg cctgtgcggc ggtaggcctg
    18661 gtgggcaaag agtctgattt gttccgcttt actgtcaaac acagcctgat cttcacctgt
    18721 atggtgggcg tgatcaccac gcttcaggct tatgtcttaa cgtggatgat tccttaatga
    18781 ttgttttacc cagacgcctg tcagacgagg ttgccgatcg tgtgcgggcg ctgattgatg
    18841 aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg ccaactggcg atgcaactcg
    18901 gcgtatcacg taattcacta cgcgaggcgc tggcaaaact ggtgagcgaa ggcgtgctgc
    18961 tcagtcgacg cggcggcggg acgtttattc gctggcgtca tgacacatgg tcggagcaaa
    19021 acatcgtcca gccgctaaaa acgctgatgg ccgatgatcc ggattacagt ttcgatattc
    19081 tggaagcccg ctacgccatt gaagccagca ccgcatggca tgcggcaatg cgcgccacac
    19141 ctggcgacaa agaaaagatt cagctttgct ttgaagcaac gctaagtgaa gacccggata
    19201 tcgcctcaca agcggacgtt cgttttcatc tggcgattgc cgaagcctca cataacatcg
    19261 tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca atcctcagtg aagcatagcc
    19321 gtcagcggat gtatctggtg ccaccggttt tttcacaact gaccgaacaa catcaggctg
    19381 tcattgacgc catttttgct ggtgatgccg acggggcgcg taaagcaatg atggcgcacc
    19441 ttagttttgt tcacaccacc atgaaacgat tcgatgaaga tcaggctcgc cacgcacgga
    19501 ttacccgcct gcccggtgag cattatgagc attcgaggga gaaaaacgca tgattatttc
    19561 cgcagccagc gattatcgcg ccgcagcgca acgcattctg ccgccgttcc tgttccacta
    19621 tatggatggt ggtgcatatt ctgaatacac gctgcgccgc aacgtggaag atttgtcaga
    19681 agtggcactg cgccagcgta ttctgaaaaa catgtctgac ttaagcctgg aaacgacgct
    19741 gtttaatgag aaattgtcga tgccggtagc actggcaccg gtgggtttgt gtggcatgta
    19801 tgcgcgtcgt ggcgaagttc aggcagccaa agcggcggac gcgcatggca ttccgtttac
    19861 tctctcgacg gtttccgttt gcccgattga agaagtcgcg ccagccatca agcgcccaat
    19921 gtggttccag ctttatgtac tgcgcgatcg cggctttatg cgtaacgcgc tggagcgagc
    19981 aaaagcagcg ggttgttcga cgctggtttt caccgtggat atgccgacac cgggcgcacg
    20041 ctaccgtgat gcgcattcag gtatgagcgg cccaaatgcg gcaatgcgcc gctacttgca
    20101 ggcggtgacg catccgcaat gggcgtggga tgtgggcctg aacggtcgtc cacatgattt
    20161 aggtaatatc tcagcttatc tcggcaaacc gaccggactg gaagattaca tcggctggct
    20221 ggggaataac ttcgatccgt ccatctcatg gaaagacctt gagtggatcc gcgatttctg
    20281 ggatggcccg atggtgatca aagggatcct cgatccggaa gatgcgcgcg atgcagtacg
    20341 ttttggtgct gatggaattg tggtttctaa ccacggtggc cgccagctgg acggtgtact
    20401 ctcttccgct cgtgcgttgc ccgctattgc agatgcggtg aaaggtgata tcgccattct
    20461 ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt atgattgcgc tcggtgccga
    20521 caccgtactt ctgggtcgtg ctttcctgta tgcgctggca acagcgggcc aggcgggtgt
    20581 agctaacctg ctaaatctga tcgagaaaga gatgaaagtg gcaatgacgc tgactggcgc
    20641 gaaatcgatc agtgaaatta cgcaagattc gctggtgcag gggctgggta aagagttgcc
    20701 tacggcactg gctccgatgg cgaaagggaa tgcggcatag tcgtttgagc cccctcaccc
    20761 taaccctctc cctcagggag tggggaccgt tcggcgctgt atgtactccc tcactctgaa
    20821 acgacaccgc actctttttt tctccctcgc ccctccgggg tgaggggaaa aggccgcact
    20881 gctcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct aaacatcgta
    20941 ctttacgaac cagaaattcc gccaaatact ggcaatatca tccgtctttg cgctaatacc
    21001 ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct gggacgataa gcgcctgcgc
    21061 cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga ctttcgcgca
    21121 ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg tacgcctgct
    21181 cacagcgccg taaactatca ggatggcgat tatctgatgt tcggcccgga aacacgcggc
    21241 ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat tccgatggtg
    21301 ccggacagcc gcagcatgaa cctgtccaat gcggtgtcag ttgtggtgta tgaagcctgg
    21361 aggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc cggatagatg
    21421 attacatcgc atccggcacg atcacaggac atcagatccc atccccatac tcaaatgtat
    21481 ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg tctggtttac
    21541 cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt tgcagcacca
    21601 cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg attttcgcgc
    21661 ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca gatttacccg
    21721 taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc gccgtttcac
    21781 ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt gctgccgggt
    21841 gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc aatgcgcgac
    21901 gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga aaacccttca
    21961 ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc accgcctgaa
    22021 tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct tccaccactt
    22081 cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc atgtagctca
    22141 gtgcactacc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg gccagcattg
    22201 gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca atttccagtt
    22261 cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt ctgcgcgggc
    22321 cgggtcatag cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc gtccttgcgt
    22381 gcacgaccta ataacgtcaa tgctgcctcg cgcgcgtttt ttccgcaata taatacttga
    22441 taaatttcct cggttattgg catttcaacg ccgaagcgat gcgccagttc gcggacttct
    22501 ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct ttgtacatcc
    22561 atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc ggtacaggta
    22621 agcaccagat cgccaagccc cgccatgccc ataaaggtgg caggatcggc acccagcgcc
    22681 gcaccaagac gcgacatttc agccagccca cgggtgatca gcgccgtacg cgcattcgca
    22741 ccaaaaccga taccgtcgga catccccgcg ccaatggcaa tgacgttttt caccgcaccg
    22801 ccaagctgca caccgatgaa atccgggttg ctgtaaacgc ggaaactttt gccacagtgc
    22861 agcaattgtt ggagatcatc ggcaaaggtc tggtcggtag aggccaacga aatagccgtc
    22921 ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc cagcggaatt
    22981 tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc cgcttccagc
    23041 cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat ctggcgcagc
    23101 acttcaccaa agacatggct gggtacgacg acgagaatat tacggctggc tgccagcgca
    23161 gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg gagaaacgcg
    23221 gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc ccagaggaca
    23281 acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga gccggcaccg
    23341 atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct tcagtacctt
    23401 cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaacc ggcgcaaggt
    23461 tcagttgcgg gaatgtaccg cgggatacca tgctggtgat gcactcacga gcatacggga
    23521 acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct tcgatacccg
    23581 cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct tcgcccaggg
    23641 aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt tgggaagatg
    23701 ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg tgcggcgcgt
    23761 tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa gtcatttcag
    23821 tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa tactgcgcaa
    23881 tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac gtgcatccaa
    23941 tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct ggggaaccgt
    24001 ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat cgatcggcaa
    24061 ctcctgaaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc aatacgggca
    24121 ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt tttaccctga
    24181 aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat aacaataagt
    24241 cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg taaattattt
    24301 gccgcgcacc aaaggcaagt tttcgccagc ccagccagcg acgccttctt tcagcacgaa
    24361 tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact gcatgccaga
    24421 accgtctatc acgataaccg gtttgtcttt gtgcttctca agctcaccaa cattgttggc
    24481 tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac ggaagtcatc
    24541 acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg tagcttcacc
    24601 acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa gaaccgccac
    24661 cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt gcataatttc
    24721 ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa ggagtatacc
    24781 tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc ggggcacgac
    24841 gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt ttgatcttca
    24901 gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc ttgagtatga
    24961 ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg atggctatgg
    25021 ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg taatggatgc
    25081 actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag tcggtctgcc
    25141 tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg gccgcatcgt
    25201 gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct ttgctaatcc
    25261 agtgctgact ggcgcggtag ataaagcgaa aaacgcaggt aaagcagtac acattatggg
    25321 tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga tggtagaact
    25381 ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg gtcgcgacac
    25441 cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg ctgcgctggg
    25501 taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg ataaccgttg
    25561 ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt tccaggccga
    25621 tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg agttcgtgaa
    25681 agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg gcgatgcgct
    25741 gattttcatg aacttccgtg ctgaccgcgc gcgcgaaatc actcgtgcat tcgtaaacgc
    25801 agatttcgac ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga tgctgaccga
    25861 atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg ttaacacctt
    25921 cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa ccgaaaaata
    25981 tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag gcgaagatcg
    26041 cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa tgagctccgc
    26101 agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca ccattatctg
    26161 taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg cggttaaagc
    26221 ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt ccgtgggtgg
    26281 acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc cggcaacggg
    26341 tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg gtgataagaa
    26401 cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt tgtcgctgat
    26461 gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg aataatccct
    26521 ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc gcaggtttgc
    26581 aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt gcgccttttc
    26641 cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg ccgcgaaaga
    26701 gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat tgaaaaagca
    26761 ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca ctctcaatca
    26821 actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc agcaaaaagc
    26881 cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg gcgagcatac
    26941 cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt tacaggctta
    27001 tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc aaacgcgtga
    27061 agaagtcgcc atgcagcgtg ctgaactgga agagaaacaa agcgagcaac aaacgctgtt
    27121 atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgagc gtaaaaagac
    27181 gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg aactgcgtgc
    27241 caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa gccgcggcga aagcgcgtgc
    27301 tgaacgagaa gcgcgtgagg cccaggcggt tcgcgaccgc cagaaagaag cgacgcgcaa
    27361 aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa ctggtggcct
    27421 gggtgcgccg cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc atcgctatgg
    27481 tgaacagcta cagggcgaac tacgctggaa aggaatggtt atcggtgcct ctgaaggtac
    27541 tgaagttaaa gcgattgccg acggtcgggt gattctggct gactggctgc aaggttacgg
    27601 tctggtggtg gtggttgagc acggtaaagg cgacatgagt ctttacggct ataatcagag
    27661 cgcactggtg agcgttggtt cgcaggttcg cgcgggccag ccaattgcac tggtgggcag
    27721 cagtggcggt cagggtcggc cttcactcta tttcgaaatt cgccgccagg gtcaggcggt
    27781 caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta acgttcttgc
    27841 atttgccgct ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg ccatcgtcat
    27901 tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc cctccgctat
    27961 ctccgtcgct gtattacccg attcaccgca cgccagagag atggcgacca aagcgcataa
    28021 cagcgggcac gaagtgttga ttcatctccc gatggcacca ctgagtaaac agccgctgga
    28081 gaaaaatacg ctgcgcccgg agatgagcag cgacgaaatt gagcgcatta ttcgtagtgc
    28141 ggtcaataac gtgccctatg ccgtggggat caacaaccat atgggtagca agatgacctc
    28201 taacctgttt ggtatgcaga aagtgatgca ggcactggag cgttacaatc tttacttcct
    28261 cgacagcgta accatcggta atacccaggc gatgcgcgcc gcgcaaggta ctggcgtgaa
    28321 ggtgatcaaa cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca tccgtgtgca
    28381 atttaatcgc gcaattgatc tggcgcgtcg caacggttca accattgcca ttgggcatcc
    28441 tcacccttca acggtacgcg tgctacaaca gatggtttat aacctgccgc cagacattac
    28501 gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacttcta caccgccgaa
    28561 aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt gcaaaccgaa
    28621 gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg aaagcatcag
    28681 ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca aacagcccga
    28741 agcggcgaag tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg atggtgcgac
    28801 cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg gtaaccgacc
    28861 ttcacgctgc gcacattggt aatcaggcgc ttatacatac cggaagtaaa tatctcggca
    28921 atcatccgtt ggcgggtaag aatatccggc tctttgcgca ccgcatggca aacacgcaat
    28981 gcttcgtaag ttatttgctg atgaaattcc ggataaatcg taattttgtc ggcataattt
    29041 cgatttaatt tctccagcag gcgggtaatc ttaatatagt gacgttgata attaaggttt
    29101 ttattccctt gtctatgcaa ccgactcact gacgtattat gcagataata tttatataat
    29161 gattgctcgg tatatcgcgc acgcagcgcg ttaaacatga attctgttgt ccagacaata
    29221 tcctgatgat gtaatccggc aataaattta atgttatttt taacaataac atcacggcga
    29281 taaaccccca tccagacaac gtgagtccaa cggcgcgaag aaagccccat ccgcagccag
    29341 tccgggccgg ttaatacgcc ggttgagcga aggcgatcgc tggggatgga ttgccaggtt
    29401 tctcccgttt cacgaaaaca ccagtcagcg ttgcactgcg ccacgtcgag gtcatcctct
    29461 aacgccatgg tcatcagcgt ttcgtacatg gtgggataga cctcatcgtc agcatcgaca
    29521 aaagcgacat atttgcccgt cgccacctcg atcccacgat tacgcgctac cgatgcgcca
    29581 gcattcgcct gatgcaacaa acgaacgtgc ggatagtttt ctgcgtaatg ctttgctatt
    29641 tcaacagaat tatccgttga accatcgtta ataataatga tttccagagc agtccaggtt
    29701 tgcgtaatta aagattccat acaagtgcgg aaatcatcgc ccgcattata taacggaata
    29761 ataacactaa gtttattggt gctgttcatc ataaattacc gacgacctct gaagttatcc
    29821 tgacgtttga caggctttgt attaagaaac tattaagcct gtgagatatt atttttacag
    29881 gtgagagagt cacttcaggg tagagagata atgagagcag ctatataaga gatcagcgca
    29941 attacgatat ctttattaca ctgacaggat tttacatatt taacggatta tcctcaggca
    30001 aaagggctgg aataccagcc cttgttcgtg ttaatcccaa ctcagaatta ctttcccgga
    30061 ctggcccgaa cgcatagcgt caaagccctt ctggaaatca tcgatagaga aacgatgggt
    30121 aatgatcggc gagagatcca gacctgactg aatcagcgcc gccatcttgt accaggtttc
    30181 aaacatctca cgaccgtaaa tacctttaat gaacaagcct ttaaagatta ctttggtcca
    30241 gtcgatagac atatcggacg gtggaatacc cagcatcgca atgcgaccgc cgtggttcat
    30301 ggtgtcgagc atggtacgaa acgctggcgg cgcaccggac atttccagac cgacatcaaa
    30361 gccttcggtc atgcccagtt ctgccatcac atcattaagg ttttctttag cgacgttgac
    30421 cgcacgggtg atacccattt tgcgcgccag ctcaaggcgg tattcgttaa catcggtgat
    30481 caccacattg cgtgcaccaa cgtgtttcgc caccgccgca gccataatac ctatcggacc
    30541 tgcaccagaa accagcacat cttcgcccac cagatcgaac gacagcgcgg tatgcacggc
    30601 gttaccgaag ggatcaaaaa tggaagccag gtcgtcggag atattgtcgg ggattttgaa
    30661 agcgttgaac gccggaatca ccagatattc ggcgaagcag ccaggacggt taacaccgac
    30721 accaatggtg ttgcggcaca gatgggtacg accgccacga cagttgcgac agtgaccgca
    30781 ggtgatatgg ccttcgccag aaacgcgatc accgattttg aagcctttca cttcctgacc
    30841 aatacctacc acttcaccga catattcatg gcctacgacc atcggcaccg ggatggtttt
    30901 ttgcgaccac tcatcccagt tatagatgtg aacgtcagtc ccgcagatgg ctgttttacg
    30961 gattttaatc agcagatcgt tatgcccgag ttccggtaca ggaacgtcgg tcatccagat
    31021 gccctcttcc gctttcagtt tggataacgc tttcatctca catcctcagg cgataacgcc
    31081 cagttgttta ccaatacgcg tgaatgcttc tactgcacgc gtaatttgct caggggtatg
    31141 cgccgcagac atctgggtac gaatacgcgc ctgacctttc ggaacgaccg gatagaagaa
    31201 accggtgacg taaatgccct ctttttgcag ctcacgggca aatttctgcg ccactactgc
    31261 atcgccaagc atgaccggaa taatggcatg atcggctccc gccagggtaa agcccgccgc
    31321 cgacatttgc tcacggaact ggcgcgcgtt cgcccacaga cggtcacgca gttcgctgcc
    31381 cgcttcgacc atctccagta ctttgatgga cgcggcaaca atggccggtg ccaacgagtt
    31441 ggagaacagg tacggacgag aacgctggcg cagccactca accacctctt tgcgcgccgc
    31501 ggtataacca ccagaagccc cgcccagcgc tttaccaagc gtaccggtga taatatcgac
    31561 ccggcccatc acatcgcagt attcatggga accacgacca ttttcaccga caaaaccgac
    31621 cgcgtgggag tcgtctacca tcaccagggc atcgtattta tctgccagat cgcaaacgcc
    31681 cttcagattg gcaatcacgc cgtccatcga gaatacacca tcggtggcaa tcagcacatg
    31741 acgcgcaccg gcttcacgcg cttctttcag acgagcttca agctcctgca tatcgttgtt
    31801 ggcatagcga tagcgtttag ctttacacag acgcacacca tcaataatag acgcgtggtt
    31861 cagtgcgtcg gagataatgg cgtcttccgc gcccagcagc gtttcaaaca ggccaccgtt
    31921 agcatcaaag caggaagagt agagaatcgc atcttccatc cccaggaagg ccgccagttt
    31981 ttgttcaagc tctttatggc tgtcctgagt gccgcaaata aaacgcaccg aagccatgcc
    32041 gaaaccgtga gaatccattc ccgcctttgc cgccgcaatc agatcaggat gattcgccag
    32101 cccgagatag ttgttggcac aaaagttaat gacgtggctt ccatcagcca cagtgatatc
    32161 tgcttgctgc gcagacgtaa taatgcgctc ttctttaaac aacccttccg cccgtgcggt
    32221 ttccagatcg ttggttaact gctgataaaa ttctccacgc attgcgattc tccagacagg
    32281 gcaaattcca gcacatatta cccaaactta taggtcgcga cgagatgacg cgttaacact
    32341 tctgcaaaat tcaggataaa taacgtgtaa cttgatgtct ctcaacacct acagatagta
    32401 gaaaaactgt acagttttgg ctgggcataa tgatgattga ttttactgca ttgatataaa
    32461 aagaaaaaat gatgccaaag catcattttt cctatctcag ttggtatcaa aacgctatcc
    32521 actaatggag ggaaacctat tttcttgaaa ggaaaattct cagcgcctgc atttttgccc
    32581 ctaaagtgtt actcctgaag cagcgaaaca aatcgaacca ttttcctctc cccagatagt
    32641 ttaggttgaa gagatcgggg cgtttgtaat agcacatgac aaaaatgccc tggtcatcgt
    32701 ccacaatgtt attatttagt gttatttttt gactttccag aactaattta tagaattctt
    32761 tccatttatg ctgtgaacca acaattgcgc ccccaataat atatacatga ttgccaatca
    32821 tgaaatcaaa aacctgttgt tgagatgtga cagtaagccc tttttttata gtgaaaagat
    32881 gcatcttact ttcatcaaat gggaaatccc aaatttt
//
LOCUS       DAZGXZ010000047        32614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000047 DAZGXZ010000000
VERSION     DAZGXZ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.047"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..91)
                     /gene="yojO"
                     /locus_tag="ACHKJF_002286"
     CDS             complement(<1..91)
                     /gene="yojO"
                     /locus_tag="ACHKJF_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP0949637.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISN"
     gene            200..694
                     /gene="napF"
                     /locus_tag="ACHKJF_002287"
     CDS             200..694
                     /gene="napF"
                     /locus_tag="ACHKJF_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP0949638.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            684..947
                     /gene="napD"
                     /locus_tag="ACHKJF_002288"
     CDS             684..947
                     /gene="napD"
                     /locus_tag="ACHKJF_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP0949639.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            944..3430
                     /gene="napA"
                     /locus_tag="ACHKJF_002289"
     CDS             944..3430
                     /gene="napA"
                     /locus_tag="ACHKJF_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP0949640.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3437..4132
                     /gene="napG"
                     /locus_tag="ACHKJF_002290"
     CDS             3437..4132
                     /gene="napG"
                     /locus_tag="ACHKJF_002290"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP0949641.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4119..4982
                     /gene="napH"
                     /locus_tag="ACHKJF_002291"
     CDS             4119..4982
                     /gene="napH"
                     /locus_tag="ACHKJF_002291"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP0949642.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4979..5428
                     /gene="napB"
                     /locus_tag="ACHKJF_002292"
     CDS             4979..5428
                     /gene="napB"
                     /locus_tag="ACHKJF_002292"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP0949643.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5438..6040
                     /gene="napC"
                     /locus_tag="ACHKJF_002293"
     CDS             5438..6040
                     /gene="napC"
                     /locus_tag="ACHKJF_002293"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP0949644.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6053..6676
                     /gene="ccmA"
                     /locus_tag="ACHKJF_002294"
     CDS             6053..6676
                     /gene="ccmA"
                     /locus_tag="ACHKJF_002294"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP0949645.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6673..7335
                     /gene="ccmB"
                     /locus_tag="ACHKJF_002295"
     CDS             6673..7335
                     /gene="ccmB"
                     /locus_tag="ACHKJF_002295"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP0949646.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVQAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7377..8114
                     /gene="ccmC"
                     /locus_tag="ACHKJF_002296"
     CDS             7377..8114
                     /gene="ccmC"
                     /locus_tag="ACHKJF_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP0949647.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8111..8320
                     /gene="ccmD"
                     /locus_tag="ACHKJF_002297"
     CDS             8111..8320
                     /gene="ccmD"
                     /locus_tag="ACHKJF_002297"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP0949648.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8317..8796
                     /gene="ccmE"
                     /locus_tag="ACHKJF_002298"
     CDS             8317..8796
                     /gene="ccmE"
                     /locus_tag="ACHKJF_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP0949649.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8793..10736
                     /gene="ccmF"
                     /locus_tag="ACHKJF_002299"
     CDS             8793..10736
                     /gene="ccmF"
                     /locus_tag="ACHKJF_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP0949650.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10733..11290
                     /gene="dsbE"
                     /locus_tag="ACHKJF_002300"
     CDS             10733..11290
                     /gene="dsbE"
                     /locus_tag="ACHKJF_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP0949651.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11287..12339
                     /gene="ccmH"
                     /locus_tag="ACHKJF_002301"
     CDS             11287..12339
                     /gene="ccmH"
                     /locus_tag="ACHKJF_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP0949652.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQVTAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12374..13021)
                     /gene="narP"
                     /locus_tag="ACHKJF_002302"
     CDS             complement(12374..13021)
                     /gene="narP"
                     /locus_tag="ACHKJF_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP0949653.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13341..15932
                     /gene="yejO"
                     /locus_tag="ACHKJF_002303"
                     /pseudo
     CDS             13341..15932
                     /gene="yejO"
                     /locus_tag="ACHKJF_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16036..16112)
                     /locus_tag="ACHKJF_002304"
     tRNA            complement(16036..16112)
                     /locus_tag="ACHKJF_002304"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16076..16078),aa:Pro,seq:ggg)
     gene            complement(16187..17947)
                     /gene="yejM"
                     /locus_tag="ACHKJF_002305"
     CDS             complement(16187..17947)
                     /gene="yejM"
                     /locus_tag="ACHKJF_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP0949654.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17967..18194)
                     /gene="yejL"
                     /locus_tag="ACHKJF_002306"
     CDS             complement(17967..18194)
                     /gene="yejL"
                     /locus_tag="ACHKJF_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP0949655.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18376..19383
                     /gene="yejK"
                     /locus_tag="ACHKJF_002307"
     CDS             18376..19383
                     /gene="yejK"
                     /locus_tag="ACHKJF_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP0949656.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19522..19806)
                     /gene="rplY"
                     /locus_tag="ACHKJF_002308"
     CDS             complement(19522..19806)
                     /gene="rplY"
                     /locus_tag="ACHKJF_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP0949657.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19931..21691)
                     /gene="radD"
                     /locus_tag="ACHKJF_002309"
     CDS             complement(19931..21691)
                     /gene="radD"
                     /locus_tag="ACHKJF_002309"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0949658.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21841..22536
                     /gene="rsuA"
                     /locus_tag="ACHKJF_002310"
     CDS             21841..22536
                     /gene="rsuA"
                     /locus_tag="ACHKJF_002310"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP0949659.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22564..23754
                     /gene="bcr"
                     /locus_tag="ACHKJF_002311"
     CDS             22564..23754
                     /gene="bcr"
                     /locus_tag="ACHKJF_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP0949660.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSHFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            24087..24431
                     /gene="yejG"
                     /locus_tag="ACHKJF_002312"
     CDS             24087..24431
                     /gene="yejG"
                     /locus_tag="ACHKJF_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP0949661.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(24435..26024)
                     /gene="yejF"
                     /locus_tag="ACHKJF_002313"
     CDS             complement(24435..26024)
                     /gene="yejF"
                     /locus_tag="ACHKJF_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP0949662.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(26026..27051)
                     /gene="yejE"
                     /locus_tag="ACHKJF_002314"
     CDS             complement(26026..27051)
                     /gene="yejE"
                     /locus_tag="ACHKJF_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0949663.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(27051..28145)
                     /gene="yejB"
                     /locus_tag="ACHKJF_002315"
     CDS             complement(27051..28145)
                     /gene="yejB"
                     /locus_tag="ACHKJF_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP0949664.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(28146..29960)
                     /gene="yejA"
                     /locus_tag="ACHKJF_002316"
                     /pseudo
     CDS             complement(28146..29960)
                     /gene="yejA"
                     /locus_tag="ACHKJF_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(30042..31598)
                     /gene="pdeN"
                     /locus_tag="ACHKJF_002317"
     CDS             complement(30042..31598)
                     /gene="pdeN"
                     /locus_tag="ACHKJF_002317"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0949665.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(31779..32345)
                     /gene="mepS"
                     /locus_tag="ACHKJF_002318"
     CDS             complement(31779..32345)
                     /gene="mepS"
                     /locus_tag="ACHKJF_002318"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP0949666.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
BASE COUNT         7696 a         8070 c         8931 g         7917 t
ORIGIN      
        1 catttgaaat gtgagcaaaa gcccgttttt gccacgcttc ccgccactct tttgatcctg
       61 ctacaggttt taccccgatc ggggtatgca tctttgacac atcctttaat atcttagcgg
      121 ctataaaaat ggcttattaa ttatgcggtt tatttggtcg ctctcaattt tcagagcgcg
      181 ttaatgatgg aaggtcaatg tgaagattga tgcatcccgt cggggcatac tcactggtcg
      241 ctggcgcaaa gccagtaacg gtatccgtcc accctggtcc ggtgatgaat ctcattttct
      301 gacccattgc acccgttgcg acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg
      361 cggcgcgggc ggctatccga gcgttaattt caaaaataac gagtgcagct tctgttacgc
      421 ctgcgcgcag gcttgccccg aatcgctatt ttctccgcgc cacaccaggg cctgggattt
      481 gcagttcacc attggggacg cgtgcctggc gtatcagtca gttgaatgcc gccgctgtca
      541 ggatagctgt gagccaatgg caattatctt tcgcccgacg ctctccggaa tctaccagcc
      601 gcaacttaat agccaacttt gcaacggatg tggagcatgt gcagccagct gtccggtatc
      661 agccataact gcggagtatc tccatgcaca ctaactggca agtttgcagc ctggtcgtgc
      721 aggccaaaag cgaacgaatt tcagacatca gcacccaact gaacgccttt cccggctgtg
      781 aagttgctgt cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa gcagaagaca
      841 gcgaaacgct gatccaaacc attgagtcag tacgcaacgt agagggcgtg ctggcggtgt
      901 cgctggttta tcaccagcag gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg
      961 tagctttatg aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc tcagcgtgcc
     1021 gggcgttgcc cgcgccgttg ttggtcagca ggaagccatt aaatgggata aagcgccgtg
     1081 ccgtttctgc ggtactggtt gcggcgttct ggtcggaacg cagcaggggc gtgtggtggc
     1141 ctgtcagggc gacccggacg caccggttaa ccgtggcctg aactgcatta agggctattt
     1201 cctgcccaag atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc gtatgaaaaa
     1261 cggtaaatat gacaaagaag gcgaatttac cccaatcacc tgggatcagg ccttcgatgt
     1321 gatggaagag aaattcaaaa ccgccctgaa agaaaaagga ccggaatcga tcggtatgtt
     1381 cggttctggt cagtggacta tctgggaagg ttatgccgcg tccaagctgt tcaaagcggg
     1441 cttccgttcg aacaacatcg acccgaacgc acgtcactgt atggcgtcgg cagtagttgg
     1501 ctttatgcgt acctttggta tggatgagcc gatgggctgc tatgacgaca tcgagcaggc
     1561 tgacgcgttt gtgctgtggg gcgctaacat ggcggagatg cacccgatcc tctggtcacg
     1621 catcactaac cgtcgtctct ctaaccagaa cgtcaccgtg gcggtgcttt ctacctacca
     1681 gcatcgtagc ttcgagctgg cggataacgg catcatcttt acgccgcaat ctgacctggt
     1741 gatcctgaac tacatcgcca actatatcat tcaaaacaat gcgataaatc aggacttctt
     1801 cagcaagcac gttaacctgc gcaaaggggc gacggacatc ggctacggtt tacgtccgac
     1861 ccatccgctg gaaaaagcag cgaagaatcc aggttctgac gcctccgaac cgatgagctt
     1921 tgaagattac aaagccttcg ttgccgagta tacgctggaa aaaactgccg aaatgaccgg
     1981 cgtgccgaaa gaccagttag aacaactggc gcagctgtat gccgatccga acaagaaagt
     2041 catctcctac tggacgatgg gcttcaacca gcatactcgt ggcgtgtggg ccaacaacct
     2101 ggtctacaac ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg gtccgttctc
     2161 cctgaccggg cagccttccg cgtgtggtac tgcgcgtgaa gtgggcacct ttgctcaccg
     2221 tctgcctgcg gacatggtgg tgactaacga gaaacaccgt gatatttgcg agaagaagtg
     2281 gaatatcccg agcggcacca ttccggcgaa aatcggtctg cacgcggtgg cacaagaccg
     2341 tgcactgaaa gacggcaagc tgaatgttta ctggaccatg tgtaccaaca acatgcaggc
     2401 cgggccgaac attaatgaag agcgtatgcc gggctggcgc gatccgcgca acttcatcat
     2461 cgtctccgat ccgtatccga cagtcagtgc gctggcagcc gacttgatcc tgccgaccgc
     2521 aatgtgggta gagaaagagg gcgcttacgg taacgccgaa cgccgtactc agttctggcg
     2581 tcagcaggta caggcgccag gcgaagcgaa atcggatctc tggcagttag tccagttctc
     2641 ccgccgcttc aaaactgaag aagtatggcc ggaagagctg ctggcgaaga aaccggaact
     2701 gcgtggcaaa acgctgtacg aagttctgta tgccaccccc gaagtgagca aattcccggt
     2761 atccgaactg gcggaagatc agctgaacga tgaatcccgc gagctgggct tctatctgca
     2821 aaaagggctg ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg atctcgcgcc
     2881 gttcgatgac taccacaaag cgcgcggtct gcgctggccg gtggtgaacg gtaaagaaac
     2941 acagtggcgt tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag gctataagtt
     3001 ctacggtaaa ccggatggca aagcggtgat cttcgcactg ccgttcgaac cggcggcgga
     3061 agcaccggat gaagagtacg acctgtggct ctctaccgga cgcgttctgg agcactggca
     3121 caccggcagt atgactcgcc gtgtgccgga actgcaccgc gccttcccgg aagcggtctt
     3181 gtttattcac ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca aagtgaaagt
     3241 ggtttctcgc cgtggcgaag tgatctcgat tgttgaaacg cgcggtcgta accgtccgcc
     3301 acagggcctg gtgtacatgc cgttcttcga cgccgcacag ctggttaaca aactgacgct
     3361 ggatgcgacc gatccgctct cgaaagagac ggacttcaag aagtgcgcag tcaaactgga
     3421 gaaggtgtaa gccattatgt cccggtcagc gaaacctcaa aatggtcgcc gccgctttct
     3481 gcgcgatgtc gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc tggggttaca
     3541 acagcaaacc gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca taaacgagaa
     3601 cgcctttgcc agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc cttacgacac
     3661 cttaaaactg gcgacgctgg cctctggtct gtcggcgggc acgccatatt ttgtcgcacg
     3721 ggatattcct tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc caagcggtgc
     3781 gctggatcgt gagattgaat cgatcgacga cgcgcggatg gggctggcgg tactggtgga
     3841 ccaggaaaac tgtctcaact ttcaggggct gcgctgcgat gtttgttatc gcgaatgccc
     3901 gaaaattgat gaggccatca ccctggagct ggagcgcaac acgcgtaccg gtaagcacgc
     3961 ccgctttctg ccgacggttc acagcgacgc ctgtaccggt tgcggtaagt gcgaaaaagt
     4021 atgcgtgctg gaacaaccgg caatcaaggt gttaccgctg tcactggcga aaggggagtt
     4081 aggtcaccat taccgcttcg gttggctgga ggggaacaat ggcaaatcgt aaacgtgacg
     4141 ccgggcgcga ggcgctggag aaaaaaggct ggtggcgcag tcaccgttgg ctggtgttac
     4201 gtcgcctttg tcagttcttc gtgctgggga tgtttttgag cggtccatgg tttggtgtgt
     4261 ggatcttgca cggcaactac agcagtagcc tgttattcga caccgttccg ctgaccgatc
     4321 cgctgatgac gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg gcgttgaccg
     4381 gtgcggtgat tatcaccgtg ctctacgccc tggcgggaaa gcgattattt tgcagctggg
     4441 tctgcccgct gaacccgatt accgacttag cgaactggtt acgcaggcgc tttgacctca
     4501 atcagtctgc gacgatccct cgccacattc gctacgtgct gttggtggtc attctgctgg
     4561 gatcggcctt aactggcacg ctcatctggg aatggatcaa ccccgtatct ctgatggggc
     4621 gcagcctggt aatgggcttc ggcagcggcg cgctgcttat tctcgcgctg tttttatttg
     4681 atctactggt cgttgaacac ggctggtgcg ggcacatttg tccggttggc gcgctgtatg
     4741 gcgtgctggg tagcaaaggt gtgattaccg ttgcggccac cgatcggcag aaatgtaacc
     4801 gctgtatgga ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc ccggtgctgg
     4861 atgaacaaag cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt cgctgcgtgg
     4921 atgtctgttc tgaggatgta tttacaataa ctacacgatg gagttcggga gcgaaatcat
     4981 gaaaagccat gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg ccctggtggt
     5041 aagcggcgcg gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg aagtatccgg
     5101 gacacaggaa ggggccattc ggatgccaaa agagcaggaa cggatgccgc tgaactatgt
     5161 gaatcagccg ccaatgatcc cgcatagcgt tgaaggttat caggtaacga ccaataccaa
     5221 ccgctgcttg cagtgccacg gtgtcgaaag ttatcgcacc actggcgcgc cgcgtatcag
     5281 tcctactcac tttatggaca gcgacggcaa agtgggcgcg gaagtggctc cgcgtcgtta
     5341 tttctgtctg caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg ggaatacctt
     5401 taccccatca aaaggttacg ggaaataaga ggttattatg ggaaattctg accgtaagcc
     5461 tggtctgatt aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg cgctggggac
     5521 gctgctgttg atcggttttg ttggcggcat cgtcttctgg ggtggcttta acaccgggat
     5581 ggaaaaagcc aataccgaag agttctgcat tagctgccac gaaatgcgca acacggtgta
     5641 tcaggaatac atggattccg tgcactacaa caaccgtagc ggcgtccgtg cgacctgtcc
     5701 ggattgtcac gttccgcacg agtttgtgcc gaagatgata cgcaagctca aagcaagtaa
     5761 agagctgtat ggtaaaattt ttggcgttat tgacacgccg cagaaatttg aagctcatcg
     5821 tctgacgatg gcacagaatg agtggcggcg catgaaggac aataactcgc aggagtgccg
     5881 taactgtcac aacttcgagt atatggatac aaccgcccag aaatcggttg ccgcgaagat
     5941 gcatgaccag gcggtgaaag atgggcaaac ctgtattgat tgccataaag ggatagcgca
     6001 caagctgccc gatatgcgtg aagtcgagcc aggtttttaa cagggttatt gcgtgggtat
     6061 gcttgaagcc agagagttac tttgtgagcg ggatgaacga accttattta gtggcttgtc
     6121 atttacgctg aacgcaggag agtgggtaca aatcaccggt agcaacggcg cggggaagac
     6181 aacgcttctc cgtttgctga cggggttgtc tcgccctgac gcaggcgagg ttctctggca
     6241 agggcagccc ttgcatcagg tacgcgacag ctaccatcaa aacctgttat ggataggcca
     6301 tcagccgggg atcaaaaccc ggctgacggc gttagaaaat ctgcactttt atcatcgcga
     6361 tggcgatacc gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg gattcgaaga
     6421 tattcctgta aatcagctct cggccgggca acaacgccgc gtcgctttag cgcgtctgtg
     6481 gctgacccgt gccacgttat ggatcctcga cgagcctttt accgcgattg acgttaacgg
     6541 cgtcgatcgt ctgacccagc gtatggcgca gcatacggag caggggggga ttgtgattct
     6601 gactacccac cagccgctca acgttgctga aagtaaaatt cgccgcattt cactgacgca
     6661 aacgagggcc gcatgatgtt ctggcgcatt ttccgtcttg agctgcgtgt agcgtttcgc
     6721 catagcgccg aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac cctttttccg
     6781 ctcagtatcg gtccggagcc gcaactgctg gcgcgtattg caccgggcat tatctgggtt
     6841 gctgcgctgc tttcatcctt gctggcgctg gaacgactgt tccgtgacga tttgcaggac
     6901 ggcagtcttg aacaattgat gttgttgccg ttacccttgc ccgccgttgt gcaggcgaag
     6961 gtgatggcgc actggatggt aaccggtctg ccgttactca tcctttcgcc actggtagca
     7021 atgctactgg gaatggatgt ttatggctgg caagtgatgg cgctgacgct tctgctggga
     7081 acgcctacgc ttggctttct cggtgcaccg ggcgtggcgc tgacagtggg acttaagcgc
     7141 ggtggtgtgc tgctcagcat actggtgtta ccgctgacta tcccattact catcttcgcc
     7201 accgccgcga tggacgcggc ttccatgcat ttgcccgttg acgggtatct ggcaatttta
     7261 ggcgcgttgc tggcaggcac cgcgacatta agtccttttg cgacggcggc agcgttacga
     7321 atcagcattc aataacgctg gattactgat ttttgttcga gtctggtatc gaaactatgt
     7381 ggaaaacact gcatcaactg gcgatcccac cacggctgta tcaaatctgt ggctggttta
     7441 taccgtggct ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc tggggattcg
     7501 gctttgctcc ggctgattat cagcagggaa atagctaccg cattatctac ctgcatgtgc
     7561 ctgcggcgat ctggtcgatg ggcatttatg catcaatggc agtggcagcg tttattggcc
     7621 ttgtctggca gatgaaaatg gccaacctgg cggtggcggc gatggccccc attggtgccg
     7681 tgtttacctt tattgccctg gttaccggct ctgcatgggg aaaaccgatg tggggcacct
     7741 ggtgggtatg ggatgcacgt ctgacttctg aactggtgct gctttttttg tatgtgggtg
     7801 tgattgccct gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg gcaggtatcc
     7861 tggtgctgat tggcgtggtg aatctgccga ttattcatta ctccgtggag tggtggaaca
     7921 ccctgcatca gggatcaacg cggatgcagc aaagtatcga tccggcgatg cgttcgccgc
     7981 tgcgctggtc gatttttggc ttcctgctcc tgtctgccac gctgacgctg atgcggatgc
     8041 gtaatttgat tttgctgatg gaaaaacgcc gtccgtgggt gagtgaactg atactgaaaa
     8101 gaggccgtaa atgacccctg catttgcttc ctggaatgaa tttttcgcaa tgggcggtta
     8161 cgcctttttt gtctggctgg cggtggtgat gaccgttatt ccgctggtgg ttttggtcgt
     8221 gcactcggtg atgcagcatc gcgcaattct gcgtggtgtg gcgcaacagc gggcgcgtga
     8281 ggcgcgttta cgtgctgcgc aacagcagga ggctgcatga atattcgccg taaaaaccgc
     8341 ttgtggattg cctgtgctgt tctggcaggg ttggcactga ctatcggtct ggtgctatat
     8401 gcgctgcgct cgaatatcga tctcttttat acgccggggg aaattctcta cggcaagcgt
     8461 gaaactcagc aaatgccgga agtcggtcag cgtctgcgcg ttggcgggat ggtgatgccg
     8521 ggtagcgtgc agcgcgatcc caattcgcta aaagtaactt tcaccattta cgacgctgaa
     8581 ggttcagtag atgtctctta cgaaggcatt ttgccggatc tgttccgtga agggcagggc
     8641 gttgtggtgc agggcgaact ggaaaaaggc aatcatatcc tcgcaaaaga agtgctggcg
     8701 aaacacgacg aaaactacac gccgccagaa gttgagaaag cgatggaagc caatcaccgt
     8761 cgcccggcga gtgtttataa ggacccagca tcatgatgcc ggaaattggt aacgggctgc
     8821 tgtgtctggc gctaggaatt gcgctgctgc tgtccgtgta tccgctatgg ggcgtagcgc
     8881 gcggagatgc gcgcatgatg gcgtcttccc gcttgtttgc ctggctgctg tttatgtctg
     8941 tggctggcgc atttctggta ctggtcaatg ccttcgtggt caacgacttc accgtcacct
     9001 atgttgccag caactccaat acccagcttc cggtgtggta tcgcgtggcg gctacctggg
     9061 gcgcgcatga aggctcgctc ctgctgtggg tgctgctgat gagcggctgg acctttgcgg
     9121 tagcgatttt tagtcagcgt attccgctgg atattgtggc ccgcgtactg gcgataatgg
     9181 ggatggtcag cgtcggcttt ttgctgttca ttctctttac ctctaacccg ttctcacgca
     9241 cgttgccgaa cttcccgatt gaagggcgcg atcttaaccc gctgttacag gatccggggc
     9301 tgatcttcca tccgcctctg ctctatatgg ggtacgtggg gttctcggtg gcgtttgctt
     9361 ttgccattgc ttctttgttg agcgggcgtc tggacagcac ttatgcgcgt tttactcgtc
     9421 cgtggacgct ggcggcgtgg atcttcttga cgctcggcat tgtgctcggt tccgcatggg
     9481 cctattacga actcggctgg ggcggctggt ggttctggga tccggtagaa aacgcctcgt
     9541 ttatgccgtg gctggtgggg actgcgctga tgcactcact ggcggtcact gaacaacgcg
     9601 ccagcttcaa agcgtggaca ttactgctgg caatcagtgc cttctcgttg tgtctgctgg
     9661 ggactttcct ggtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcc tctgatccgg
     9721 cacgcggtat gtttatcctc gcctttatgg tactggtgat tggtggttcg ctgctgctgt
     9781 ttgccgcgcg tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg tcgcgggaat
     9841 ctctgctgtt agcgaacaac gttttgctgg tcgccgcgat gctggtggta ttactgggga
     9901 cgctgctgcc gctggtgcac aagcaactgg gactgggcag tatttcgatt ggcgaaccgt
     9961 tcttcaacac catgtttacc tggctgatgg tgccgtttgc gctgctgctt ggtgtcggtc
    10021 ctctggtgcg ctgggggcgc gatcgcccac gtaaaatccg caatttattg attatcgcct
    10081 tcatctcaac gctggtgctg tcgctgctgc tgccgtggct gttcgaaagc aaagttgtgg
    10141 cgatgacggt gctcggcctg gcaatggcct gctggattgc tgtgctggca attgcggaag
    10201 ctgcgctgcg tatttcacgt ggcacgaaaa ccaccttcag ttattgggga atggtggcgg
    10261 ctcaccttgg gctggcagtg acaattgttg gcattgcctt tagtcagaac tatagcgttg
    10321 agcgtgatgt gcgcatgaag tccggcgata gcgtcgatat tcatgaatat cgcttcacct
    10381 tccgtgatgt caaagaggtg actggcccga actggcgtgg cggtgtggcg actatcggcg
    10441 taacgcgaga gggtaaaccg gaaacagtgc tgtatgcgga aaaacgttat tacaacactg
    10501 ccgggtcgat gatgaccgaa gcggcgattg acggtggcat cacgcgtgac ctgtacgccg
    10561 cgctcggtga agagctggaa aacggcgcgt gggctgtgcg tctttactac aaaccatttg
    10621 ttcgctggat ttgggcgggc gggctgatga tggcgttggg cggactgctg tgtctgtttg
    10681 atcctcgcta tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggct gtatgaagcg
    10741 caaagtattg ttaattccgt tgattatctt cctggcgatt gccgcggcgc tgctgtggca
    10801 gttggcgcgt aatgccgaag gggatgatcc gaccaatctg gaatcggcgc tcattggcaa
    10861 gcctgtgccg aagtttcgtc tcgaatcact ggacaatccg gggcagtttt atcaggcgga
    10921 tgtgctgact cagggcaaac cagtgctgct taacgtctgg gcgacctggt gtccgacctg
    10981 ccgtgcggaa catcaatatc tgaatcagct ttctgcgcag ggcatccgag tggtcggcat
    11041 gaactataaa gacgatcgcc agaaggcaat cagctggctg aaagagctgg gcaatcctta
    11101 cgcgctaagc ctgtttgatg gcgacggcat gttagggctg gatctcggtg tctatggcgc
    11161 gccagaaacc ttccttattg acggcaacgg catcattcgc tatcgccatg cgggcgatct
    11221 caatcctcgc gtctgggaag aagagatcaa gccgctgtgg gagaaataca gtaaggaggc
    11281 cgcacaatga ggtttttatt gggcgtgctg atgttgatga tctccggctc agcgctggcg
    11341 accatcgacg tgttgcagtt taaagatgaa gcacaggaac aacagttccg tcagctcact
    11401 gaagaactgc gctgcccgaa atgccagaat aacagcattg ccgattccaa ctcgatgatt
    11461 gccaccgacc tgcgccagaa agtgtatgaa ctgatgcagg aaggtaaaag taagaaagag
    11521 attgtcgatt atatggtggc gcgttacggc aacttcgtca cttacgatcc gccgttaacg
    11581 ccgctgaccg tgctgctgtg ggtgctgcca gtagtggcta ttggcattgg cggttgggtc
    11641 atttacgccc gctcgcggcg tcgggtacgc gtggtgccgg aagcgtttcc tgaacaaagc
    11701 gtgccggaag gtaagcgtgc cggatatgtc gcttatttgc cgggcattgt ggtggcgtta
    11761 attgtggctg gcgtcagcta ctaccagacc ggaaattatc aacaggtgaa aatctggcag
    11821 caggtcacgg cacaggctcc ggcgttgctg gacagggcgc tggatccgaa agccgatccg
    11881 ctcaacgaag aagagatgtc gcgtcttgcg ctggggatgc gtactcaact gcaaaaaaat
    11941 ccgggagata tagaaggctg gattatgttg ggccgcgttg gcatggcgct gggtaacgct
    12001 agtatcgcca ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa cagcgatgcc
    12061 gcactgggtt atgctgaagc gttgacacgt tcatctgatc ccaacgacaa ccgcctcggc
    12121 ggcgaactgc tgcgccagtt ggtgagaagc gaccacagca atatccgtgt gttaagcatg
    12181 tatgcgttta atgcctttga gcagcagcga tttggcgaag ccgttgccgc gtgggagatg
    12241 atgttgaaac tcttacctgc caacgatact cgccgtgcgg tgattgaacg tagtatcgcg
    12301 caggcgatgc aacatttgtc gccgcaggag agtaaataaa aagaggcccg atggtgatgc
    12361 catcgggcta tttttattgt gccccgcgtt gttgcaggaa cagaatggtg gctgccacgc
    12421 gtgagcggac attgagtttg cgcagcagat tgcgaatatg tacttttact gtctgctctg
    12481 aaatattcaa caccgaggca atctgtttat ttgacagccc ctgtgccagc tcgtgcagaa
    12541 catccagctc gcgctccgtc agcacgctga agggatcttc ttccgcgcca aacatttcac
    12601 gttcacgtaa gtactgattg acgcgttcgc taaagacttt gctgcctttc gctccagcac
    12661 gaattgcttc cagcaatact tccgggtcgc tgtctttcaa cagataaccg tctgcgcctg
    12721 cgtctatcag tgcaaagaca tcgctggagg catctgatac ggtcaggata ataatttgcg
    12781 cggtaacgcc atccctgcgc aaggcattga gggtatccag gccactcata cctttcatat
    12841 tgagatccag caagatcacg tcgatatcca gtctattcgc cagatcgatc gcgctcgcgc
    12901 cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag ctccagtaac tgacgaacac
    12961 cgcgtcgcat aagtggatga tcatccacaa tcatcacctg aaaaggcgtt gcttcaggca
    13021 tagtagtctc ctgaggtttt attagaatca ttatgtttga gcgtagcgtt ggtaacgcta
    13081 ccaggcgcgt gaatgttacc ccattttatg tatggacatg tagtgaaaac tacaaccttg
    13141 tcgttcgctt tggcatcatt ttcattataa agacggaatt ttgcatcagc ttctcatcaa
    13201 gaaaaacaac taaaaaatca atcacaatac ttgtgcattt ctttaaacag tttttaaaaa
    13261 ctgactccgg gtatggagct atgggtattt tccgtaccca atgcttttaa cagcaattaa
    13321 tttcatagga tgaaagctca attcatcaat ctggttctgt ttctctttgt cgttccgcaa
    13381 tatctgttct ggtggctaca gcgttatatt cacccatagc attggcatca acagttgagt
    13441 atggtgagac agttgatggt gttgtcctgg aaaaagatat ccagctggtt tatgggaccg
    13501 ccaataatac gaaaatcaat cctggcggag aacagcatat aaaagaattt ggtgtaagta
    13561 gtaatactga aattaaagga gggtatcagt acattgaaat gaatggtacc gcagaatact
    13621 cagtattaaa tgacggttat caaattgttc aaatgggtgg cgcggcaaac cagactaccc
    13681 tcaataatgg tgtgctacag gtttatggcg cagcgaatga tcccacgatt aaaggcgggc
    13741 gcttaatcgt tgaaaaagat gggattaccg tccttgccgc tatcgaaaag ggaggattac
    13801 tggaggttaa agagggggga ttagcgattg cggtagatca gaaagcaggc ggtgctatta
    13861 aagcaagcac gcgggtcatg gaggcattcg gaacaaaccg tctcggtcag ttcgaaatca
    13921 agaatggtat tgctaacaat atgctgttgg aaaacggcgg aagtttgcga gttgaagaaa
    13981 atgacttcgc ttataatact actgtagata gtggcggctt actggaggtt atggatggcg
    14041 ggactgtaac tggcgttgat aaaaaagcag gcggaaaatt aattgtctca acgaatgcgc
    14101 tggaagtgag tggtacaaac agtaaaggcc aatttagtat aaaagatggt gtgtcaaaaa
    14161 attatgaact ggatgatggt tccgggctca ttgttatgga ggacacgcag gccattgata
    14221 ctatccttga tgagcatgcc actatgcaat cgctgggaaa ggatactggt acgaaagtgc
    14281 aggcaaatgc ggtatatgat ctcggtcgat cagatcagaa tggaagtatc acgtattcct
    14341 caaaagccat ttctgaaaat atggttatca acaatggccg cgctaacgtc tgggctggca
    14401 caatggttaa cgtttcagtc agagggaatg atggcattct tgaggtcatg aagccgcaaa
    14461 taaattatgc acccgcaatg ttggtgggta aggtagtggt ttctgagggc gcttctttta
    14521 gaacgcatgg tgccgtggat accagcaaag cggacgtttc gctcgaaaat agcgtatgga
    14581 ccatcattgc cgatatcact acgacgaacc aaaacaccct cctcaactta gccaaccttg
    14641 cgatgtctga cgcaaatgtg attatgatgg atgagccagt gactcgttca tcagtgacgg
    14701 caagtgcgga aaatttcatt acgttgacca ccaataccct gtcgggaaac ggcaattttt
    14761 atatgcgtac cgatatggct aatcatcaga gcgatcagct caacgtcacc ggtcaggcaa
    14821 caggtgattt caaaatattc gtgacggaca ccggtgccag cccggcagca ggagatagcc
    14881 ttacactggt aacaacgggc ggcggtgatg ctgcatttac gttgggcaat gccggaggcg
    14941 ttgttgatat cggtacgtat gaatatacct tgctggataa tggcaaccat agctggagtt
    15001 tggcagagaa tcgcgcgcaa attacccctt caaccactga tgtgctgaat atggcggcag
    15061 cacaaccgct ggtatttgat gcagaactgg acaccgtgcg tgagcgtctt ggtagcgtaa
    15121 aaggcgttaa ttacgatacg gcgatgtgga gttcggcaat taacacccgc aacaacgtga
    15181 ccactgatgc gggcgctggc tttgagcaaa cattgaccgg gctgacgctc ggtatcgata
    15241 gccgtttctc ccgtgaagaa accagcacaa ttcgcggctt gttctttggt tactctcatt
    15301 ctgatattgg ttttgatcgc ggcggcaaag gcaatgtcga tagctatacc ctgggggctt
    15361 atgccggttg ggagcatcag aacggtgcct atgttgatgg agtggtgaaa gttgaccgtt
    15421 ttgccaacac catccattgc aagatgagta atggggcaac agcgtttggc gattacaata
    15481 gtaacggcgc gggtgctcat gtcgagagcg ggttccgttg ggttgacgga ttgtggagtg
    15541 ttagacccta tctggccttt accggcttta ccacagatgg tcaggactac acgttatcaa
    15601 acggcatgcg cgcggatgtg ggaaataccc ggatattacg cgctgaagcg ggaacggcgg
    15661 taagctatca catggacctg caaaacggta cgacgctgga accctgactg aaagccgccg
    15721 tgcgtcagga atacgccgat tctaaccagg tgaaagttaa tgacgatggc aaatttaata
    15781 atgatgtggc tggaacccgt ggcgtttatc aggctgggat aaggtcatcg tttaccccga
    15841 cgttaagcgg tcatttgtca gtcagctatg gcaatggcgc aggggtagaa tcgccgtgga
    15901 atactcaggc gggtgtggtc tggacgttct gataacaaaa aataaacagg ctgtgatgtg
    15961 tcacggtctg tttatcgaat taattgcaga tataaaaaaa accaaccgta aaggttggtt
    16021 ttttcttggg atttttggtc ggcacgagag gatttgaacc tccgaccccc gacaccccat
    16081 gacggtgcgc taccaggctg cgctacgtgc cgactcgtgg ctgctaatac taccgttttc
    16141 cacaccgatt gcaagtaaga tattttgcta actgatttat aattaatcag ttagcgataa
    16201 aacgcttctc gtctgtcagc acttgcaata acaaacttaa ctgtggtttt tcatctttca
    16261 ctctttcacc acgtaagttg taagtgcggt atttaccgtt attgttcagc accagcgtct
    16321 ttttcggggt ggtaattgcc agcgtatcgt tatccgctgc agtaacccag taatgacggc
    16381 gttgagggtt gaacaaatcc tgaccttgcg aatattcgct ggcaggtgtg ctgacatgta
    16441 gcaggcgttg catcagcgtc gtcatcagat cggtatgatc agtcagagca ttaatacgct
    16501 gcgccggcgt gcctggccag tgaatcacta atggcacctg cagatgaccg tgggaccagt
    16561 caaaggtttc ttcgtcttcg ctcagcggaa taccccgacc ggcagtgata atcaccaccg
    16621 tattgtccag tttgccagaa tcacgcagtg cattgagcac gcggttgatc tggtcatcga
    16681 cattgcctgc cgcccggcta tatttccgtg caaatgcctg ctgattgctg tcgtcaatgt
    16741 tagtgccatt gaaagagacc cacgagaacc agcggttatc ttcttgtgcg tagcggccca
    16801 gccagttgat ccactgcgtg gcggtctgct cgtcggattg ggtgcgtacg ctcggcatcg
    16861 agaaatctga caacaatgcc tggcgataca gcgggctggt aaagccatct gatgagaata
    16921 atcccagctg atagccttgc tgattaagcg cagtaattaa cgccgcaggg gtacgggtcg
    16981 acagaatgcc gtccatatag ctcggcgaga tgccatagaa caggccaaag atgccgttgt
    17041 ctgtagtgtt gccggagctc atatggcgcg tgaacgaaat attttgctca gcaaaacctg
    17101 ccagcgcagg catctgcttc tcgaagcgtg agtagttcag gccatcgaca gtaatcaaca
    17161 gcacattctg cccggtgccc atatcgcgat agcgcagttc gcttaacgga tactgaacgg
    17221 aaacggcgtc tggattacct tgctcaataa gacggcgttg atactcctgc gcatcaagca
    17281 gaccatgctt ctcaagaaaa cgtcgcgccg tcatcgggta cgaaagcggc aggttagcgc
    17341 gctgcatggt gatcgggcga tagaagttgg catcggccca gatatacacc acatgcgagg
    17401 cgataaaggc gataaataag aatgcggcca gcgggcgcgc gaagcgccga cgacgcgtca
    17461 ggctgcgcag cttttgccag ctccacgtcg caaacaccag ttcaagcaat aaaataaccg
    17521 gcacgctgat gaacatcagc tgccagtcgc gcgccatctc attttcgtct gggttgataa
    17581 ccagttgcca gacgatggga ttaagatgga gatggaaacg agtaaagact tcgctatcga
    17641 tcagtaatag cgtcattccc gccgttgcca gaatgacgga caaaaacctc atcagcctct
    17701 gggagccgac gataaaggtc agcgggaaga ggatcagcaa gtaggtggcg aacaccagga
    17761 agctgaaatg gccgataatg cttacgtagg aataaatgcg accagcaagc gttgtcggcc
    17821 agtcggcgat aaacaggtaa cggctgccaa tgacgagcga aagcagaata ttgaacagtg
    17881 caaaccagtg cccccaactg accatctggg agactttttc acggtagcgc tgacgatgag
    17941 ttaccataac ctgttatctg tttcccttag tgcgctttgt cttcgttgat agaggactgt
    18001 aaggcgcggg caaaagagtt ggcaattgcc tggcgttggg ccggggcaat gctggtattg
    18061 ataaggttag tgaccatgtt gcctaacacc atcagggaaa ggtcggtcgg agccttatgt
    18121 ttttccagta cgttgagcag ctctgcgagc aattgttcaa cctgttcatc actgtagcgg
    18181 gaaatttgtg gcataaatcg aaatcagtta gttgatgaaa gggcaacata ttaccgtagc
    18241 aacggttttt tttctgcttt tttatgcgtt aaatcattct cgttacgctg ccttctgtaa
    18301 aatgaccgcc atcttttatt gcgtcgctta accggcggtg gttgaatacc gcccggtctt
    18361 aaaggagagt ttatcatgag tctggatatc aaccagattg ctctgcacca gcttatcaag
    18421 cgtgatgagc agaaccttga gctggtgttg cgcgattcat tgctggaacc gacagaaacc
    18481 gtcgttgaga tggtggcaga actgcatcgg gtctatagcg ccaaaaataa agcttacggc
    18541 ttgtttagcg aagagagtga actggcgcag acgctgcgct tacagcgtca gggcgaagag
    18601 gactttctgg catttagccg cgcggcaacc gggcgcctgc gtgacgaatt agcgaaatat
    18661 ccttttgctg acggtggatt cgtgctgttt tgccactacc gttatctggc ggtggagtat
    18721 ctgctggtgg cagttctgag caacctgagc agtatgcgcg ttaacgaaaa tctcgacatc
    18781 aatccaaccc attatcttga tatcaatcat gcggatattg ttgcgcgtat cgatttaacc
    18841 gaatgggaaa ccaatccaga gtccacccgc tatctcactt tcctgaaagg gcgagtaggg
    18901 cgcaaagtgg cggatttctt tatggatttc ctcggggcca gcgaagggtt aaacgcgaaa
    18961 gcgcaaaacc gtgggctgtt gcaagcggtt gatgatttca ctgccgaagc acaactggat
    19021 aaagcagagc gacagaacgt gcgccagcag gtttatagct actgtaatga gcagctgcag
    19081 gcaggcgaag agatcgaact ggagtcgctg tcgaaagagc tggcaggcgt cagcgaagtg
    19141 agtttcacgg agtttgcggc tgaaaaaggc tacgaactgg aagagagctt cccggcagac
    19201 cgtagcacac tgcgtcagtt gacgaagttt gccggtagcg gcggtggctt gactattaac
    19261 tttgatgcga tgctgttagg tgaacgcatc ttctgggatc cggcaaccga taccctgacc
    19321 attaaaggca cgccgccgaa tttgcgtgac caattgcagc gccggacgtc tggcggcaat
    19381 taatcggttt tgtgttctga atgccagatg cggcgtgaac gccttatctg gcctgcaaaa
    19441 aatagcaata ccgtaatcag gcataatctg cagatacaaa aaaccccgcc ggagcggggt
    19501 tttttacaac ttattcagca attaagcgcg aacgaagtcg atgtgctgca gcttcggttt
    19561 gtacgggtga cgctgtacgt cctgagcttt aactttgatt tctttaccgt caacaacgat
    19621 ggtcagaact tcgctgtaga attcagcttt agcttgcatg ttcatgactt tgtcgtgatc
    19681 cagctcgata gccagcggcg cttctttgcc accgtagatg attgccggga acttgttagc
    19741 ggcacgcagg cggcggctcg cacccttacc ctgctcttta cgtacttctg cgttgatagt
    19801 aaacatttct ttctctctta aattaatcct gctacaggcg acccagcaac aggtaagtga
    19861 tctgctttgc gtatgcaaaa gcgggcgaga ttctatactc aatcgcttta tacatcaatc
    19921 aaaactacca ttaaccgcgt aattcgtgcg cccgacgaaa acgaccttcg taatcgaaca
    19981 ctttttcacg cacttgccag tactgacctt tcatgcgggc aacgacaaaa tccgggtgtc
    20041 gcaataaggc ttgctgggcg aggatatcgg cggcggtgat ccagcgcaga gggatgcccg
    20101 gtgtgcgcgt atgcgggcgg ataaaaagct gctcgaaggc agttcgctgg gcgggcgttt
    20161 gcagacggaa acgctcactc acatccgcgc cgtcttcatc gtaataggtg attttcaacc
    20221 attcgccttt ctcgtcgtgc tcatgctgca aagacatgcc gctacagcgt aataccagcg
    20281 catctttcag tcgtagcgcc gcttttaaca tatcatccgg atcaaccagt accgtgtcac
    20341 attcgcggca gcggcgagcg gcaatatcat tttctgcgtt acattgcggg caatttttaa
    20401 aacggaaacg gaagtcacac tgttcgcgat gaccgtcgtc atcttcaaac catccctgac
    20461 agcggcgacc aaagtgttca atcaatgtcc cgtcggcggt cgttttcccc caaaaggtgt
    20521 tggcaaaacc gcaggcaggg cagaaaacct gtaccggaac gttgtcactt ttgcctttcg
    20581 gtgtaccaac ttccggcgcg tagagatcgt gaggattacc cgcataatca aggattaaac
    20641 aatcagtctt gcccggagcg agacgcagac cgcgcccgac aatttgttgg taaagactca
    20701 ctgattcggt agggcgcaga atggcgataa gatcgacgtg cggggcgtca aatccggtgg
    20761 tcagtactgc gacgttgacc agatagcgaa aacgctgggc tttaaaatct tcaattaaca
    20821 catcgcgctc agcgccgggg gtgtcgccag taatcagtgc tgcatcttcg gcaggcagta
    20881 atcccacaat ctcttttgcg tgttcaaccg tcgcggcaaa aatcatcacc cctttgcgcg
    20941 ttgcagcaaa ctccataatc tggctgatga tgtgcggggt aatacgttgt tgttttttca
    21001 gctcacggtt gagatcggct tcgctgaaca gcccgttact ctgtgcctgc aagcggctga
    21061 aatcgtattg cactactggc atatccagtc gttctggcgg cgtcagatag ccgtgtttaa
    21121 tcatataacg cagcggcagc tcataaatgc agtcacggaa aagggctttc tcatcgccgc
    21181 gtaccatgcc gtgataatga aactggtaga tccagccttt gcccaatcga aaaggcgtgg
    21241 cagtcagccc cagcaggcgt aagtggggat tcacttttgt caggtgagtg aggatttgct
    21301 gatactggct ctcttcatcg tcaccaatac ggtgacattc atcgacaatc aacagcgaaa
    21361 attcaccctg aaaggcatca agattacggg cgacagactg cacgctgcca aataccactt
    21421 taccgtggct ctctttgcgc tttagcccgg cggcaaaaat atcggcttcc agccccagcg
    21481 cctgatactt tgcatggttt tgcgccacca gttctttaac gtgtgccagc accagtacgc
    21541 gaccacgagc cagccgtgcc agttccgcta tcaccaggct tttacctgcg ccggtgggca
    21601 gcacgataac ggcaggggtt ttatgacgac gaaaatggtt gagcgtggca tccacggctt
    21661 cttgctgata tgggcggagt gtaaaaatca tggtctcgcg taggtagatt attctgctaa
    21721 tagtatgcca cggttctttt tcccttgagg gaagttatta gaccgctata ctgggcagac
    21781 aacagttcag attttcgggt cattacccga cctccgtttt ttacaggcaa aatcacacac
    21841 atgcgacttg ataaatttat cgcacagcaa ctcggcgtta gccgtgctat tgccgggcgt
    21901 gaaatccgcg gcaatcgtgt caccgtcgat ggcgaaatcg tccgtaatgc agcgttcaaa
    21961 ctgctccctg aacatgatgt cgcttacgat ggcaacccgc tggcgcagca acacggtcca
    22021 cgttacttca tgctcaataa gcctcagggc tatgtttgct ccacggacga ccctgatcac
    22081 ccaacggtgc tctattttct tgatgaaccg gtagcgtgga aactgcatgc ggcggggcgg
    22141 ttggatattg ataccaccgg tctggtgctg atgactgatg atggtcagtg gtcgcaccgc
    22201 attacttctc cgcgccatca ttgcgagaag acctatctgg tgacactgga atcacctgta
    22261 gctgacgata cggcagagca atttgctaaa ggcgtgcagc tgcataacga aaaagatctc
    22321 actaagcctg cggtgctgga agtgattacc ccaacgcagg ttcgtctgac catcagcgaa
    22381 gggcgttatc atcaggtgaa acgcatgttc gccgccgtgg gtaaccacgt ggttgagctg
    22441 catcgtgaac gtattggcgg tattacgctg gatgctgatt tagcccccgg tgaatatcgt
    22501 ccgttaactg aagaagaaat tgccagcgtc gtctaacttt tcaatgactt tcaggagccc
    22561 gttgtgacca cccgacagca ttcgtcgttt gctattgttt ttatccttgg cctgctggcc
    22621 atgttgatgc cgctgtcgat tgatatgtat ctgcccgcgc taccggtaat ttcagcgcag
    22681 tttggcgtac cggcgggcag tacgcagatg accctcagta cttatattct gggctttgcg
    22741 ttggggcagt taatctacgg gccgatggca gacagcttcg ggcgtaagcc ggtggtgctc
    22801 ggcggtacgc tggtgtttgc cgccgccgcg gtggcgtgtg cgttggcaaa caccatcgat
    22861 cagctgattg tgatgcgttt cttccacggg ctggctgcgg ctgcggccag cgtggtcatt
    22921 aacgccctga tgcgcgatat ttacccgaaa gaagagttct cgcggatgat gtcgtttgtc
    22981 atgctggtga ccactattgc accgttgatg gcaccgattg tcggcggttg ggtgctggtg
    23041 tggctgagct ggcattacat cttctggatc ctggcattag cggcgattct ggcttcggca
    23101 atgattttct tcctgattaa agaaacctta ccaccggagc gtcgtcagcc atttcacatt
    23161 cgtaccacta ttggtaactt tgcggcgctg ttccgccata aacgtgtcct gagctacatg
    23221 cttgccagtg gtttcagctt tgccgggatg ttctcattct taagcgccgg accgtttgtt
    23281 tatattgaaa ttaaccacgt cgcgccggaa aactttggtt attactttgc gctaaacatt
    23341 gtttttctgt tcgtgatgac catctttaac agccacttcg tccgccgcat tggcgcgtta
    23401 aatatgttcc gctcggggtt gtggatacaa tttattatgg cagcgtggat ggtcatcagt
    23461 gcgctgctgg ggctgggatt ctggtcgctg gtggttggcg ttgcggcgtt tgtgggttgc
    23521 gtgtcgatgg tgtcatccaa tgcgatggcg gtcattcttg atgagtttcc ccatatggcg
    23581 ggaacggcat cttcgctggc aggaaccttc cgttttggca taggggcaat tgttggcgca
    23641 ttgctttctc ttgcgacctt taactctgca tggccgatga tttggtcaat tgcattctgc
    23701 gcaaccagct ccattctctt ctgtctgtac gccagtcggc cgaaaaaacg gtgatctatt
    23761 gcacaacgag gaaggtaaaa ggcttccttt gttgatgcat gtcaaccaca aatctatcat
    23821 ttccccgata tatgtttatt ttatgtaaaa tcaatttatg taaaaagtca catcattgta
    23881 gttaaaaagg ttgagttaga tcgcagaaac gggtacatat agccccgcaa acgtgaccac
    23941 gcccgcagat attacttaaa tcagagccat agaggccacg caggcgaggc atcaatcttt
    24001 acgatctgta taaagacgga ttgttgatga tgtgttaaaa ttgatgtaaa caaattgtga
    24061 agtgaatgtg cttccgggga aaataagtga cttcattaca actctcaatc gtccatcgac
    24121 tgccgcagaa ctatcgctgg tctgctggtt tcgcaggttc gaaggttgaa ccgattccgc
    24181 aaaatggacc gtgcggtgac aacagcctgg tggcgcttaa attgcttagc ccggatggtg
    24241 ataatgcatg gtcggtgatg tataaactaa gccaggcgtt aagcgacatc gaagttccat
    24301 gttcggtgct ggagtgtgaa ggtgagccgt gcctgtttgt aaatcgccag gacgagtttg
    24361 ctgcaacatg ccgattgaaa aattttggtg tggcaattgc cgaacccttt tcaaactaca
    24421 atccttttta agcgtcagct caacgccagt agctgacgcg tatactcctg ctgcggtgtg
    24481 gcaaacacgc gcgcgcacgg tccttgttcc actacttccc cttgtcgcag tacgataacc
    24541 tgatgacata acgcgcggac aacgtgcaaa tcgtggctga taaacaaata ggccagttga
    24601 tgcttttgtt gcaatgattt caatagcgtc aatatttgcg cctgtaccgt tttgtcgagt
    24661 gatgatgtcg gttcatcaag tatgatcagc gagggcttaa gaattaatgc cctggcaatc
    24721 gcaatacgtt gtcgctgacc accagagaac tccgccggat aacggtggcg tgtttcagga
    24781 tctaatccca cttcatgcat cacggctatc acttgttgtt cgcgttgtgc ggcagaaagc
    24841 gtcggctggt gaactcgtaa gccttcctca ataatctgca aaacgttgag tcgtgggttg
    24901 agcgaggagt ttggatcctg aaataccacc tgaatgcgat gacgaatagg taacagctgg
    24961 cggcgattta aattttgcag tggctgaccg tcaaagatga tgctgccctg agaattaatc
    25021 agtcgcagca gcgccagtcc cgtcgtactt ttcccggaac cggactcgcc cactaaaccc
    25081 agtgtttcac ccgctcgtag cgtaaaactg atgtttttca ccaccacatt atgatccaca
    25141 atgcgcttca aaatcccttt gcgaatgggg aaggcaacct gaagctgttc aacatccagc
    25201 aacgttgagg caggttctgg caacggcacc gggtcgcctg acggttcact gttgagtagc
    25261 ttttgtgtgt aaggatgagt gggtgaggca aatagcgtag cggcgtaatt ttgctcgaca
    25321 cagcgaccgt tttgcattac cgccacgcgg tgggccagtt ttctgacaat gctgaggtta
    25381 tgagtaataa acagcatgcc catattcagc tcgccttgca gttcgcgcaa cagttgtaaa
    25441 atctgcgcct ggacagagac gtccagtgcg gtggtcggtt catcggcaat taataattcc
    25501 ggtcgcgtta acagcgccat cgcaatcatc acccgctggc gttcgccgcc ggagagctga
    25561 tgcggataat ctgtcagccg ttttgccgcc tggcggatac caacgcgatc aaggcagtta
    25621 agaatttcgc ctcgagccgc ttcccgacgc atcccgcggt ggagtgaaag cacttcataa
    25681 agctgttttt ccagggtatg caatggattt aacgacacca tcggctcctg aaaaatcatg
    25741 gcgatcttat taccgcgtac accgcgcaac gtttgatcgc tggcatgaag cagcgattcg
    25801 ccatgaaaac gaatatcgcc ggagagatat tcaaccggcg gggaagggag caggcgtaaa
    25861 attgacagcg cggtaacgct tttgcctgaa cctgactcac ccaccagcgc cagcgtttcg
    25921 ccagcctcaa tctgtagtga aacatcattg actactgtac gtacggtttg ctgatggcga
    25981 aaacccaccg acaaattttc aatcgctaac agagtttgcg tcatgctaca ccgccttatt
    26041 aggatcaaat gcgtcgcgga cggcttcacc aataaagatc agcaaagaca ataatatcgc
    26101 caccgacaag aaggcggtga tcccaagcca cggagcctga aggttatttt tcccttgtaa
    26161 cagcagttca ccgagtgacg gtgaaccgag cggcagaccg aagccgagga aatcgagtga
    26221 ggtcagggtg gttatcgaac tacataaaat aaacggtaaa aaggtgaggg tcgcgaccat
    26281 ggcattaggc aacatatgac gcaggatgat actgcgatcg ctgacgccaa gcgcctgcgc
    26341 cgcacgaatg tagtcgaaat tacgggtacg taaaaactcc gcccgcacca cgccgaccag
    26401 actcatccag ccaaacaaga cagtaattgc cagcagccac cagaagttag gctgtacgac
    26461 gctggaaagt aaaataatca aaaacagcgt cggcatcccc gaccatactt caataaagcg
    26521 ttgcccccag agatcgactt taccgccgta atagccttgt agcgcccccg ccagcacgcc
    26581 catcacgctg gaacagagag tcagcatcag gccaaacaga accgagatcc gcgtgccata
    26641 gagaatacgt gccagcacat cgccgccgtt ggcatccgtt cccagccagt tttgccggga
    26701 gggtggagaa gggaagggct tatcggtagc aaagttgata ctggtagcac caaagcgaat
    26761 cggtgcccac agtacccagc cgttattttc cagccgttgt ttcagccacg ggtcctgata
    26821 atcagcctga cttgccagcg ggccgccaaa atcgctttcg ctgtagtttt tcaacaacgg
    26881 gaaataccaa ctgccgtcat aacgtaccag caacggttta tcgttggcga taagttcaga
    26941 acacaaactc aaaccaaaca aaacgaggaa aatccataac gaccagtagc cgcgacggtt
    27001 atggcgaaaa cgcgcccaac gggcctgatt gacggggctg agtcgcgaca ttaacgtccc
    27061 tcaaaatcta tacgcggatc gaccagcgta tagctgatat cactgacaat attcagcagc
    27121 aggccaatca gggtgaaaat atacaaggta ccaaacatta caggataatc gcgcgagacg
    27181 gtcgcttcgt agcccagtaa gccaaggcca ttgagtgaaa acatcacttc aatcagcagc
    27241 gagccggtaa aaaacatgct gataaacgtc gccggaaaac cggcaatcac cagcagcatg
    27301 gcgttgcgga acacatgttt ccagagaata tttttttcac ttaccccttt cgcacgcgcg
    27361 gtcaccacgt attgcttgcg cacttcatca aggaatgagt ttttggtcag catggtcagc
    27421 gccgcaaagc caccaatcac tgtcgccagc accggcagcg tgatatgcca cagataatcg
    27481 gtgattttct gataccacgg cagcgaatca aagttagcgg aaaccaggcc gcgtagaggg
    27541 aacaggtcga aatagctgcc gcccgcgaag aagacaatca gcaggatggc aaacaaaaag
    27601 gccggaatgg cgtagccgat gatgataaat gcgctactcc agacgtcaaa gcggctccca
    27661 ttataaacag ctttgcgaat gcctaacgga atcgacacca gatagataat cagcgtgctc
    27721 cacaatccga gggtgatgga aaccggcaga ctgtctttaa tcagcgtcag caccgaggcg
    27781 ctgcgaaaaa ggctatcacc aaaatcaaag cggatgtagt cccagagcat tttgaagtaa
    27841 cgttcgtgga tcggcttatc gaaaccgtag cgatgagtga tctcggcgat cacttctgga
    27901 tctaatccgc gtccgccacg gtaattactg tcgctgatat tgccgacgcc cgtttgcgca
    27961 tggctggcgc gaataccttc accgcctgcg ccgggtaata ctccggcatt accaaactca
    28021 atggcggcga tggcctggtc gaccggaccg ccaggcgcaa tttgcacgat gaaaaagttg
    28081 atagtgataa tcgcccataa cgttgggatc accagcaaca gacggcgaat cagataagcg
    28141 cccatctact ctccctgttg tctggctgac ggcagtttgg ccgctttatt aacgtcatac
    28201 caccaggtat cgatacccag gctgtaaaca gggcgtacag cgggttggga gaatttatcc
    28261 caccaggcga gacggtcttc cgccatgtac cacattggca gcatgtaata attccacgtt
    28321 aatacgcgat ccagtgctcg ccctaacggc agtaattttt ctttatttcc ctgcgcggca
    28381 ataatttgat tgatcagcga gtcgataacc ggactttgca cgccgggggc attataagtg
    28441 gaattgatat attccgatga ccaggaaatc tgtaaatcgg aactgggcca cggcatcgcc
    28501 cgccatacgc gcggcatcat gtcatagtcg cgactgcgca tgcggttggt gatttgcgag
    28561 ttatccacct tgcgaatgtc catgttgata cccagccgtt gcaggctgtg ctggaacggc
    28621 aatacccact gactattgct gcttgcggga agcaataatt caaagctgag tggctgaccc
    28681 gttgtggcat taacgcgttg ctgacccttc agcacccagc ccgcttcgtt gagaagtttg
    28741 tcggctttta acaggttgtc acgatcgtag ccatcgcctt tagataccgg cggctggtag
    28801 atttgtgtga agacttctga cggtagatct tttttcattg gtgccagaag caccagctcc
    28861 gcggcgtcgg ggtaatttct ggccgcgtat tcggtattct gaaaataact gttcgtgcga
    28921 ctccaggcat tgtaaaacaa cgccttgttc atccattcaa agtcaaaggc gagggtgatt
    28981 gcttcccgga cccggcgatc gctgaatacc ggacgttgga tattaaacgc cagtcagcgc
    29041 gtatcctggg ctgattcgtt cttttgctca tctttgatga tgtatttttt atcgaaattt
    29101 ttaccggtat aacgcgtggc ccagttttta gcgtcgtttt ccatacgcaa atcaaaggca
    29161 cctgctttaa acgcttcaaa ggcgacatta tcatcgaggt aataatcgta gcgaatggtg
    29221 tcgaaattcc agcgtccacg gtttaccggt aagtttgctg cccagtaatc tttcacgcgg
    29281 gaatagacaa tattttgccc cattttccag gacgtaattc ggtacggacc actggcaagc
    29341 ggaggcgtgg cgagcggatc gctaagtttg tgatccttcc agtacttttc tggaaatacc
    29401 ggcagcgaaa acagactcaa catatcttct ttgccgggtt tagctaactc aatgcgcacg
    29461 gttaacggcg caatggcttt gacggtggtg cctttgtaga ccagacgaaa ttgcggcacg
    29521 ccttcggtca taaatttttg aaaagtaaac tctacatcgc gggcagtaat gggcgaacca
    29581 tcatgaaaac gggcgcgcgg attaatagcg acctccaccc aggaataatc gtcagcatag
    29641 cgtgcgcttt cagcaatcag cgggtaataa ctgcctggtt cgtcatcgga agtcgtaaat
    29701 agcgtgtcgt acagctgctc ggtgcgtgcg cccgggttgc cgcgcagtgc atagcggttg
    29761 aaattatcga aggtaccgag ggctgacaac gttatctgcc caccttttgg cgcggcgggg
    29821 ttcacataat caaaatggtt gaaattaaac gcgtaccggg gttcgcccag cacggcaaag
    29881 gcatagcttt ccttgatagc ctgcgcctgc gcaccaaagg tgaacagagc gataaacagc
    29941 agcagtatgc gcacaatcat ctgcaacgga tttcctttta ctcatcatta gccgcaatcc
    30001 ggcatgcttt cactatgaat aataagggac gtaagcacac cttaccactg cggcgtatac
    30061 ggtttcttca gccagcgaac aaaatcgtcc agcggtaacg ggcgactaat ccagtagcct
    30121 tgcatgaaat taacgccgcg ttcgcttagc catcgcgctt gttccggcgt ttcgacccct
    30181 tcagcaaccg tcagcatatt gaggcgtttc gccagcgtca gcaccgcgtc aagtacgggt
    30241 gaagtgatcg tttccgtacc gatggcgttg ataaatccac ggtcaatttt cagataatcg
    30301 agcgtaaaac gctcaagata gataagcgcg ctgtgcccgg tgccgaagtc atcaatagca
    30361 atttctacgc ctaccgagtg cagccaggcg aagagttgtg tggcttcttg ctctttcagc
    30421 atatcgcgct cggtaatttc cagcacaatc tggaaatggt gtgcgggtag ggaagtgagc
    30481 agtttctgga tatctgcttt aaagctttcg ctgtgcagat ggtccggcgc aatgttaatg
    30541 ccaaatttga cgcctaccgg cagcactttt tctaattctg cggcatcgcg ggcaattaac
    30601 tcaaacagat gctgagtcag cggcacaatc atcttttgcg attcggcaaa gttaatgaag
    30661 gcatccgggg gaatttctcc cgcgacagga tgccgccagc gtagcagtac ttccaggccc
    30721 gttactcgca aagcttgtgt atccaccacc ggttgatacg ccacgtaaaa ttgttcgcgc
    30781 ttgatggcgg tcatgatttc tctgccgggg cgcatacgta cgctcatcag gtaatagcag
    30841 agcaggccaa cgacagttcc actcatgccg cccagtaaaa atgcgtacca cacatcgttc
    30901 catgtccagt catccgcata aagccgaacg gtcagaggaa tgcccgcaat tttagtttca
    30961 cggaccggca tgtcggttaa ttcattaacg ttcatcaaac gtgaagaaaa ggtagatagc
    31021 gcagtattgc caataatgag ggcgacgcca tcgtaatctt cctgccgtga actataaaag
    31081 agagaaggcg tcaggttgag attcagagcg gcaaacacac cgctattttt cagcaaaggg
    31141 ttgcgatacc agatgacgat tgcgggtttg ttcggcacca tcggcgtgcc gggtaagatc
    31201 gccatatcga cgtttttatt aatgtcgagc gccggaatca attcattgag tggaatgtcc
    31261 atctcaccag tcgcagatga acagaatgtt tttttatctt tcaccagcac aaacgtacgg
    31321 acattcatgc taaacgccgc gcgagcggtc agttcggggt tagcctgctg gcaggtatct
    31381 aaggtcagcg gctgaagccg gtcagtagtg gatttcaggt cggcaaaata ggtatcgaga
    31441 tacttttgta cgtcgataat cagtgtgtcg tatttgactt cgtgcttatg ccaggccact
    31501 aaaaattgaa ggcaactcac cagtatcgca atcatcacgc ctgcaacaat gcaggtaagc
    31561 aggagcttac gtccagaatt gggagcgcgt atgaacatga ttccgttaat ttcctgaaat
    31621 tacgacaaca tttaaaagat aacaatgacc ggactatagc ctggatcaag acgcggaaac
    31681 atcattttct ctgatgaatg aaaacaaaaa agcactgcct aagcagtgct ttcagttaac
    31741 tcgtcaggat agccaaggga ttgcatccaa acggtttatt agctgcggct gagaacccgg
    31801 cgtgcttcgt tgtaacgctt cttccagtac ggttcattca tgctggaaat aataacacca
    31861 ctgctggtgg aagcatggac aaactgattg ttgccgatat aaataccgac atggcgtccc
    31921 gttgaaccgg cacggaacag aactaaatca cccgtacgca aattactgcg ggaaacagat
    31981 ttacccattt cctgctgttc gtaagtcgaa cgtggaagtt ctaagccaaa ttgctcacgg
    32041 aatgtacgct gtacgaaacc agaacaatcg ataccttttt tagtgctgcc gcccagacga
    32101 taacgtacgc ctttccagtc agcatactga tccataattc gcgattttac gtcgacatta
    32161 cgaaccaggt tttcaaattc atcctgagaa gcttgcagtg atgatgtttc actacccact
    32221 gcacgtgtct caggatgcat attctttgcg gtgttatttg cactacatgc agaaagcaga
    32281 accgctactg caatcgcggg aatcccgcgc aagatatatc tcaaaatcgg ttgagatttg
    32341 accatgttgt ttgttttccc ttgaagtcct taacgacaaa tatcgttata aaaaaatgcc
    32401 aagcgacacg agactaatta cctgggcatg ttgaaacaat tccttaaggc caaatctgtg
    32461 gactaacgca caaaattata tgctgtgagg ataatctacc gccgaggcaa aacgagttcg
    32521 agattacccg attggtgcgg tcttggcgag cggttttaca tgatttttta taagaaaaag
    32581 tgatacagaa agttaataag cgggattggt caat
//
LOCUS       DAZGXZ010000048        31996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000048 DAZGXZ010000000
VERSION     DAZGXZ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.048"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..197
                     /locus_tag="ACHKJF_002319"
     CDS             <1..197
                     /locus_tag="ACHKJF_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949667.1"
                     /translation="VLFILSLTTTLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVL
                     GVFPVTNIVLFLILIFFKKR"
     gene            complement(244..1131)
                     /gene="rarD"
                     /locus_tag="ACHKJF_002320"
     CDS             complement(244..1131)
                     /gene="rarD"
                     /locus_tag="ACHKJF_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP0949668.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1183..1650)
                     /gene="yigI"
                     /locus_tag="ACHKJF_002321"
     CDS             complement(1183..1650)
                     /gene="yigI"
                     /locus_tag="ACHKJF_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP0949669.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1815..2684
                     /gene="pldA"
                     /locus_tag="ACHKJF_002322"
     CDS             1815..2684
                     /gene="pldA"
                     /locus_tag="ACHKJF_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP0949670.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2817..4646
                     /gene="recQ"
                     /locus_tag="ACHKJF_002323"
     CDS             2817..4646
                     /gene="recQ"
                     /locus_tag="ACHKJF_002323"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP0949671.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQINSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4710..5330
                     /gene="rhtC"
                     /locus_tag="ACHKJF_002324"
     CDS             4710..5330
                     /gene="rhtC"
                     /locus_tag="ACHKJF_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP0949672.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5392..6012)
                     /gene="rhtB"
                     /locus_tag="ACHKJF_002325"
     CDS             complement(5392..6012)
                     /gene="rhtB"
                     /locus_tag="ACHKJF_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP0949673.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6124..7146
                     /gene="pldB"
                     /locus_tag="ACHKJF_002326"
     CDS             6124..7146
                     /gene="pldB"
                     /locus_tag="ACHKJF_002326"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP0949674.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7154..7954
                     /gene="yigL"
                     /locus_tag="ACHKJF_002327"
     CDS             7154..7954
                     /gene="yigL"
                     /locus_tag="ACHKJF_002327"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP0949675.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8030..8929
                     /gene="bioP"
                     /locus_tag="ACHKJF_002328"
     CDS             8030..8929
                     /gene="bioP"
                     /locus_tag="ACHKJF_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP0949676.1"
                     /translation="MVLLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8817..9770)
                     /gene="metR"
                     /locus_tag="ACHKJF_002329"
     CDS             complement(8817..9770)
                     /gene="metR"
                     /locus_tag="ACHKJF_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP0949677.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9888..12149
                     /gene="metE"
                     /locus_tag="ACHKJF_002330"
     CDS             9888..12149
                     /gene="metE"
                     /locus_tag="ACHKJF_002330"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP0949678.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12189..13004)
                     /gene="ysgA"
                     /locus_tag="ACHKJF_002331"
     CDS             complement(12189..13004)
                     /gene="ysgA"
                     /locus_tag="ACHKJF_002331"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP0949679.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGLDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13266..14027
                     /gene="udp"
                     /locus_tag="ACHKJF_002332"
     CDS             13266..14027
                     /gene="udp"
                     /locus_tag="ACHKJF_002332"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP0949680.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14168..15595
                     /gene="rmuC"
                     /locus_tag="ACHKJF_002333"
     CDS             14168..15595
                     /gene="rmuC"
                     /locus_tag="ACHKJF_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP0949681.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQYAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15690..16445
                     /gene="ubiE"
                     /locus_tag="ACHKJF_002334"
     CDS             15690..16445
                     /gene="ubiE"
                     /locus_tag="ACHKJF_002334"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP0949682.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16459..17064
                     /gene="ubiJ"
                     /locus_tag="ACHKJF_002335"
     CDS             16459..17064
                     /gene="ubiJ"
                     /locus_tag="ACHKJF_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP0949683.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17061..18701
                     /gene="ubiB"
                     /locus_tag="ACHKJF_002336"
     CDS             17061..18701
                     /gene="ubiB"
                     /locus_tag="ACHKJF_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP0949684.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18780..19049
                     /gene="tatA"
                     /locus_tag="ACHKJF_002337"
     CDS             18780..19049
                     /gene="tatA"
                     /locus_tag="ACHKJF_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP0949685.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19053..19568
                     /gene="tatB"
                     /locus_tag="ACHKJF_002338"
     CDS             19053..19568
                     /gene="tatB"
                     /locus_tag="ACHKJF_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP0949686.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19571..20347
                     /gene="tatC"
                     /locus_tag="ACHKJF_002339"
     CDS             19571..20347
                     /gene="tatC"
                     /locus_tag="ACHKJF_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP0949687.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20389..21171
                     /gene="tatD"
                     /locus_tag="ACHKJF_002340"
     CDS             20389..21171
                     /gene="tatD"
                     /locus_tag="ACHKJF_002340"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP0949688.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21168..21656)
                     /gene="rfaH"
                     /locus_tag="ACHKJF_002341"
     CDS             complement(21168..21656)
                     /gene="rfaH"
                     /locus_tag="ACHKJF_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP0949689.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21823..23316
                     /gene="ubiD"
                     /locus_tag="ACHKJF_002342"
     CDS             21823..23316
                     /gene="ubiD"
                     /locus_tag="ACHKJF_002342"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP0949690.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23362..24063
                     /gene="fre"
                     /locus_tag="ACHKJF_002343"
     CDS             23362..24063
                     /gene="fre"
                     /locus_tag="ACHKJF_002343"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP0949691.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24255..25418)
                     /gene="fadA"
                     /locus_tag="ACHKJF_002344"
     CDS             complement(24255..25418)
                     /gene="fadA"
                     /locus_tag="ACHKJF_002344"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP0949692.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25428..27617)
                     /gene="fadB"
                     /locus_tag="ACHKJF_002345"
     CDS             complement(25428..27617)
                     /gene="fadB"
                     /locus_tag="ACHKJF_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP0949693.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPKGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27807..29138
                     /gene="pepQ"
                     /locus_tag="ACHKJF_002346"
     CDS             27807..29138
                     /gene="pepQ"
                     /locus_tag="ACHKJF_002346"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP0949694.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29138..29752
                     /gene="yigZ"
                     /locus_tag="ACHKJF_002347"
     CDS             29138..29752
                     /gene="yigZ"
                     /locus_tag="ACHKJF_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP0949695.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29791..31242
                     /gene="trkH"
                     /locus_tag="ACHKJF_002348"
     CDS             29791..31242
                     /gene="trkH"
                     /locus_tag="ACHKJF_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP0949696.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31254..31799
                     /gene="hemG"
                     /locus_tag="ACHKJF_002349"
     CDS             31254..31799
                     /gene="hemG"
                     /locus_tag="ACHKJF_002349"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP0949697.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7434 a         8134 c         8759 g         7669 t
ORIGIN      
        1 ttgttctatt tattttaagc ctgactacga cattaaattt aagaagtata ggtatagggt
       61 taagttttgg cttttgtcaa atccttggta gatatacgat gtctctagga tggagtaagt
      121 actggggagt tttgggggta tttcctgtaa caaatattgt tctttttctt atcttaattt
      181 ttttcaaaaa aagataaata agtcagtggg taggctcttt aaacggccta cccatgttaa
      241 ttcttaactt tttctacgct gcgtataaat cgcatccatc acaaaaattg ccagcgccac
      301 ccaaataaag gcgaaagtca ccatcttatc ggcacccggt ttttcaccat aaaacgtcac
      361 agccagcagg aacatcagcg tcgggccaat gtactggaaa aagcctaacg ttgagagacg
      421 caagcgcgtg gcagcggcgg taaaacacaa cagcggtacg gtagtgacaa taccggcggc
      481 gatcagcagt aaattcagcg acatcgggtt ttgccccata tggctggttg agctgtcggc
      541 aatagcaaac aggtaaattg ccgccacggg cagcagccac atggtttcga ttaacatgcc
      601 ggtttgcgct tcaacggcaa tcttcttgcg taccagaccg tagaaggcaa aactaaatgc
      661 cagccccagc gcgataatag gtagcgaacc aaaagtccac agctggacca acacgccaca
      721 tatcgccaga atcaccgcca gccattgcat ccggcggaat cgctcgccga ggaaaatcat
      781 ccccagcaca atgttcacca gcgggttaat aaagtaacca aggctcgctt ccagcatatg
      841 gtgattgttc accgcccaga taaacagtag ccagttgcca ccaatcagca cggcagagac
      901 tgccagcata aaaattttct gtggcgtctg aatcagcgtt tttaaatagg accactggcg
      961 gcaaatgctc atcagcacca ccataaagaa aaacgaccag atcacgcgat gcgtcaggat
     1021 ttcatcggcg ggcacgtagt aaatcaactt gaagtacgct ggcgctatac cccaaataaa
     1081 ataagcggca agagcgagta atacgccctg ccgcgtttgt tttgcatcca tcgggaatac
     1141 tcatttttaa ttggtaacag cagtttacct gcttttatgt cttcaaccta ccatataggt
     1201 ggcggtggca ctggcaatat aaagctgttc ttcattgtgt aattcaacgc gggcgacggc
     1261 gactttattg cctgcacgca acaggctact ggtagcagta aaacgctcgc ccctgcctgg
     1321 gcgcagataa tcaacgcgaa gatcaatggt ccccatccgc gatagccgct ggcgtagttc
     1381 atcttcactg atggtttcgt ggcgggttaa ggtacttccc acgcacacca gaccggcggc
     1441 gacatccagc gccgacgcaa tgaccccgcc gtgcaaaatg ctttgcgccc agttgcccac
     1501 catcattggc tgatttttaa aggccagctg tgcgaactct ttttcgtaac gctccagttc
     1561 catccccaat gcgcggttaa atggcatgtg ataaacaaac atctcaccca ctaatttcag
     1621 ggcttgttca gcagtcagta cggcagacat atcatcctta cacttcattg gttaatgaaa
     1681 tgttgatttt atgcttcttt gttgttggtt tctactttag gaagggataa ctaacggcaa
     1741 tggagttaag tatgtaaaat agcccgcaga aaaacattca ccttatcaat aattcgttac
     1801 ggagaacacg accgatgcgg actctgcagg gctggttgtt gccggtgttt atgttgccta
     1861 tggcagtata tgcacaagag gcaacggtga aagaggtgca tgacgcgcca gcggtgcgtg
     1921 gcagtattat cgccaatatg ctgcaggagc atgacaatcc gttcacgctc tatccttatg
     1981 acaccaacta cctcatctac acacaaacca gcgatctgaa taaagaagcg attgccagtt
     2041 acgactgggc ggaaaatgcg cgtaaggatg aagtaaagtt tcagttgagc ctggcatttc
     2101 cactgtggcg tgggatttta ggcccgaact cggtgttggg tgcatcttat acgcaaaaat
     2161 cctggtggca actgtccaat agcgaagagt cttcaccgtt tcgtgaaacc aactacgaac
     2221 cgcaattgtt cctcggtttt gccaccgatt accgttttgc aggttggacg ctgcgcgatg
     2281 tggagatggg gtataaccac gactctaacg ggcgttcaga cccgacctcc cgcagctgga
     2341 accgccttta tactcgcctg atggcagaaa acggtaactg gctggtagaa gtgaagccgt
     2401 ggtatgtggt gggtaatact gacgataacc cggatatcac caaatatatg ggttactacc
     2461 agcttaaaat cggctatcac ctcggtgatg cggtgctcag tgcgaaagga cagtacaact
     2521 ggaacaccgg ctacggcggc gcggagttag gcttaagtta cccgatcacc aaacatgtgc
     2581 gcctttatac tcaggtttac agcggctatg gcgaatcgct catcgactat aacttcaacc
     2641 agacccgtgt cggtgtggga gttatgctaa acgatttgtt ttgatgaacg gttgagttgt
     2701 tggcaaatct ggaatccagc atccaggatt accctctcag agactaaaag cattgcagtt
     2761 tctcgcgcag gcgctgaaaa tagcgcctgt ttttatttca ggcaatcggg gtgaatgtgg
     2821 cgcaggcgga agtgttgaat ctggagtccg gagctaaaca ggttttacaa gaaacctttg
     2881 gctaccaaca gtttcgcccc ggccaggaag aaattatcga cactgtgctt tccggtcgcg
     2941 attgcctggt cgtcatgccc accggtggcg gaaaatccct ttgctatcaa attcctgcct
     3001 tattgctaaa cggccttacc gtggttgttt caccgctgat ttcgttgatg aaagatcagg
     3061 tggatcaact gcaagccaac ggcgtggcgg cggcgtgcct taactcgacg caaacccgcg
     3121 aacagcaact tgaagtgatg acaggctgcc gcaccgggca aattcgtctg ctttatatcg
     3181 ccccggaacg cctgatgctg gataactttc ttgagcatct ggcgcactgg aatccggtgt
     3241 tattagccgt tgatgaagcg cactgtatct cccaatgggg ccacgatttc cgcccggaat
     3301 atgccgcgct cggtcagttg cgccagcggt tcccgacgct gccgtttatg gcgctgaccg
     3361 ccacagccga cgacaccacg cgccaggata tcgtgcgcct gctggggctg aacgatccgc
     3421 tgattcaaat caacagtttt gaccgtccga atattcgcta catgctgatg gagaagttca
     3481 aaccgctcga tcagttgatg cgctacgtgc aggaacagcg cggtaagtcc ggcattatct
     3541 actgcaacag ccgggcgaaa gtagaagaca ccgctgcgcg cctgcaaagc aagggtatta
     3601 gcgcggcggc ctatcatgcc gggctggaaa ataatgttcg cgccgatgtg caggaaaaat
     3661 tccagcgcga tgacctgcaa attgtggtgg cgacggtggc gttcggcatg ggcatcaata
     3721 aaccaaacgt tcgcttcgtg gtccactttg atattccgcg caatatcgaa tcctattatc
     3781 aggaaaccgg tcgcgccggg cgtgatggcc tgcctgcgga agcgatgctg ttttacgatc
     3841 cggctgatat ggcgtggctg cgccgttgtc tggaagagaa gccgcagggg cagttgcagg
     3901 atatcgagcg ccacaaactc aatgcgatgg gcgcgtttgc cgaagcgcaa acttgccgtc
     3961 gtctggtatt gctgaactat tttggcgaag ggcgtcagga gccgtgcggg aactgcgata
     4021 tctgcctcga tccgccgaaa cagtacgacg gttcaaccga tgctcagatt gccctttcca
     4081 ccattggtcg tgtgaatcag cggtttggga tgggttatgt ggtggaagtg attcgtggtg
     4141 ctaataacca gcgtatccgc gactatggtc atgacaaact gaaagtctat ggcatgggcc
     4201 gtgataaaag ccatgaacat tgggtgagcg tgatccgcca gctgattcac ctcggcctgg
     4261 tgacgcaaaa tattgcccag cattctgccc tccaactgac agaggccgcg cgtccggtgc
     4321 tgcgcggcga atcctctttg caacttgccg tgccgcgtat cgtggcgctc aaaccgaaag
     4381 cgatgcagaa atcgttcggc ggcaactatg atcgcaaact gttcgccaaa ttacgcaaac
     4441 tgcgtaaatc gattgccgat gaaagtaatg tcccgccgta cgtggtgttt aacgacgcaa
     4501 ccttgattga gatggctgaa cagatgccga tcaccgccag cgaaatgctc agcgttaacg
     4561 gcgttgggat gcgcaagctg gaacgctttg gcaaaccgtt tatggcgctg attcgtgcgc
     4621 atgttgatgg cgatgacgaa gagtagtcag cagcataaaa aagtgccagg atgaagactc
     4681 cgtaaacgtt tcccccgcga gtcaaatgta tgttgatgtt atttctcacc gtcgccatgg
     4741 tgcacattgt ggcgcttatg agccccggtc ccgatttctt ttttgtctct cagaccgctg
     4801 tcagtcgttc ccgtaaagaa gcgatgatgg gcgtgctggg cattacctgc ggcgtaatgg
     4861 tttgggctgg gattgcgttg cttggcctgc atttgattat cgaaaaaatg gcctggctgc
     4921 atacgctgat tatggttggc ggtggcctgt atctctgctg gatgggttac cagatgctgc
     4981 gcggcgcgct gaaaaaagag gcagtttctg cacctgcgcc acaggtcgag ctagcgaaaa
     5041 gtgggcgcag tttcctgaaa ggtttactga ccaatctcgc taatccgaaa gcgattatct
     5101 actttggctc ggtgttctca ttgtttgtcg gtgataacgt tggcactacc gcgcgctggg
     5161 gcatttttgc gctgatcatt gtcgaaacgc tggcgtggtt taccgtcgtt gccagcctgt
     5221 ttgccctgcc gcaaatgcgc cgtggttatc aacgtctggc gaagtggatt gatggttttg
     5281 ccggggcgtt atttgccgga tttggcattc atttgattat ttcgcggtga tgccagacgc
     5341 gtcttcagag taagtcggat aaggcgttta cgccgcatcc gacattattt ttcacgcatg
     5401 cctcgccgat gctaacagcg ctcccaccag cataaacaac gagccgaaaa tcttattcag
     5461 cgccttcatc tgctttggtc ctttaatcca tagagcaatc cgttgagcaa gggtggcgta
     5521 accgatcatc acaataatat cgaccacaat agtggtgacg ccgagcacga tatactgcat
     5581 cagttgcggc tgttgcggca tgatgaattg cggaaatagc gccgccagaa acacaatact
     5641 tttgggattg gtgagattca caaaaactgc gcgctggaac aaatgtcgac gcgattgagt
     5701 agaggccagc gatttaaggt caattgcacc agcggcgcgc cactgctgga ttcccagcca
     5761 aatcaagtaa gccgcgcctg cccacttcaa cacttcaaac gcaatcactg agcgggaaaa
     5821 tagcgtcccc aaccccacgc caaccagcac aatatgaatc gccagtccgg tctgaagccc
     5881 agcaatagac gccaccgcgc cgcgataacc gtggttgagc gaggtggtca tagtgttgat
     5941 tgcaccagag cctggcgaca ggcttaaaat gatcgatgtc agcaggtagg caaaccacca
     6001 ttctaaggtc atgatgaact cccggtgtgt ctatttttgt gccacaatac gctactgtcg
     6061 cagcgttgtg tcaggcacgc taaaaaaaaa cgattttacg tggtttaaga ggcagattac
     6121 ccgatgtttc agcagcaaaa agactgggaa acaagagaaa acgcgtttgc tgcttttacc
     6181 atgggaccgc tgactgattt ctggcgtcag cgtgatgaag cagagtttac tggtgtggat
     6241 gacattccgg tgcgctttgt ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc
     6301 tgcccggggc gtattgagag ctacgtaaaa tatgcggaac tggcctatga cctgttccat
     6361 ttggggtttg atgtcttaat catcgaccat cgcgggcagg gacgttccgg tcgcctgtta
     6421 gccgatccgc atctcgggca tgttaatcgc tttaatgatt atgttgatga tctggcggca
     6481 ttctggcaac aggaggtcca gcccggtccg tggcgtaaac gctatatact ggcacattcg
     6541 atgggcggtg cgatctccac attatttctg caacgccatc caggtgtatg tgacgccatt
     6601 gcgctaactg cgccaatgtt tgggatcgtg attcgtatgc cgtcatttat ggcacggcag
     6661 atcctcaact gggccgaagc gcatccacgt ttccgtgatg gctatgcaat aggcaccggg
     6721 cgctggcgcg cgttgccgtt tgctatcaac gtactgaccc acagcagaca gcgatatcga
     6781 cgtaacttac gcttctatgc tgatgaccca acgattcgcg tcggtgggcc gacctaccat
     6841 tgggtacgcg aaagtattct ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc
     6901 acgccaacgc ttctcttgca ggctgaagag gaacgcgtgg tggataaccg catgcatgac
     6961 cgtttttgtg aactccgcac cgccgcgggc catcctgtcg aaggaggacg gccgttggta
     7021 attaaaggtg cttaccatga gatccttttt gaaaaggacg caatgcgctc agtcgcgctc
     7081 cacgccatcg ttgatttttt caacaggcat aactcaccca gcggaaaccg ctctacagag
     7141 gtttaaattt cttatgtacc aggttgttgc gtctgattta gatggcacgt tactttctcc
     7201 cgaccatacg ttatcccctt acgccaaaga aactctgaag ctgctcaccg cgcgcggcat
     7261 caactttgtg tttgcgaccg gtcgtcacca cgttgatgtg gggcaaattc gcgataatct
     7321 ggagattaag tcttacatga ttacctccaa tggtgcgcgc gttcacgatc tggatggtaa
     7381 tctgattttt gctcataacc tggatcgcga cattgccagc gatctgtttg gcgtagtcaa
     7441 cgacaatccg gacatcatta ctaacgttta tcgcgacgac gaatggttta tgaatcgcca
     7501 tcgcccggaa gagatgcgct tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc
     7561 tggattactg gagccggaag gcgtcagcaa agtgttcttc acctgcgatt cccatgaaca
     7621 actgctgccg ctggagcagg cgattaacgc tcgttgggga gatcgcgtca acgtcagttt
     7681 ctctacctta acctgtctgg aagtgatggc gggcggcgtt tcaaaaggcc atgcgctgga
     7741 agcggtggcg aagaaactgg gctacagcct gaaggattgt attgcgtttg gtgacgggat
     7801 gaacgacgcc gaaatgctgt cgatggcggg gaaaggctgc attatgggca gtgcgcacca
     7861 gcgtctgaaa gaccttcatc ccgagctgga agtgattggt actaatgccg acgacgcggt
     7921 gccgcattat ctgcgtaaac tctatttatc gtaatcgttc tttatttggt cagttgtcaa
     7981 cctgatactt cgctacaatg gatactcgtt aatcaaagag ttttccattg tggtgctact
     8041 tatcatcacc acgattctgt gggccttctc ctttagcttt tatggcgagt accttgcggg
     8101 gcacgtcgat agctattttg cggtgctggt gcgcgttggc ctggcggcac tcgtttttct
     8161 gccgtttctg cgtacccgtg gcaatagcct gaaaacagtc ggcctgtata tgctggtggg
     8221 cgcgatgcag cttggcgtga tgtatatgct gagtttccgc gcttatctct atctgacggt
     8281 ttccgaactg ctgctgttca ccgtactgac gccgctctac atcacgctga tttatgacat
     8341 catgagtaag cgccgtctgc gctggggata tgcttttagt gccttgttgg cggtgattgg
     8401 tgccgggatt attcgctatg atcaggtcac cgaccatttc tggactggct tgttgctggt
     8461 gcaactctcc aatatcactt ttgccatcgg catggtgggt tacaaacgcc tgatggaaac
     8521 tcgcccgatg ccgcagcata acgcctttgc gtggttctat cttggcgcgt tcctggtggc
     8581 ggtaattgca tggttcttgc tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg
     8641 gggcattctg gtgtttcttg gcgtggtggc ttccgggatt ggctacttta tgtggaacta
     8701 cggcgcgacg caggtggacg ccggaacgct gggaattatg aataatatgc acgttccggc
     8761 agggctgctg gtaaacctgg ctatctggca ccaacagccg cactggccaa cgtttattac
     8821 aggcgcgctg gtgatcctgg cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc
     8881 ttcacaaacg gcagatgatc gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta
     8941 actggctgac gctgctcgcc atcgcgcacg gcggcgtaca gtcggctcca caagccttcg
     9001 cccagggttt tggtcaccac cagaccctgg cgctcaaaac tctctactac ccaatgcggt
     9061 agcgcggcaa tacccatccg cgcggcaacc atctgaatca acaataaggt gttatcgacg
     9121 ctttttagtg acggactgac gcctgccggc tgaagaaaat gccgccagac atccagtcga
     9181 ctacgctgca ccggataaat taatagcgtc tcgctggcga gatcttccgg tgtaatacgc
     9241 gtttttgccg ccagtggatg gtcaggtgct aacaccagac gcacttcata gtcgaacatc
     9301 ggcgaataat gcagaccact gcgcggcaga atatcggacg tcattaccag atccagctcg
     9361 ccctgttgca acgcgggctg cgggtcaaac gtcacgcccg atttaaaatc catctctacc
     9421 tgcggccagt tcttatggaa attttctaac gcgggcgtca gccactgaat acagctatgg
     9481 cactcaatgg caatgcgcag acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg
     9541 ctaatttgcg gcagcacctg gtttgccagt tgcaacagga tttctccctg cggtgtaaag
     9601 cgtagcggct ggctcttacg cacaaatagc cggaagccaa ggcgttgttc cagatcgcta
     9661 aactggtgag acagggcgga ttgcgtctga tgcaacgtcg ccgcagcggc tgcgagagag
     9721 ccgcagttcc gcaacgcttg tagcgttttc aggtgtttta cttcgatcat gaaagtcctt
     9781 cacttcggca tgaataattt gcgcttgagg aatatacagt aaccgccaat tatggatgtg
     9841 taaacatctg gacggctaaa tactaaaaat tccataaggg gcatataatg acaattctta
     9901 atcacaccct cggtttccct cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa
     9961 gttattgggc ggggaactcc acgcgtgaag aactgctgac agtagggcgt gaactgcgtg
    10021 ctcgtcactg ggatcaacaa aagcaagcgg gtatcgacct gctgccggtg ggcgattttg
    10081 cctggtacga tcatgttctg accaccagtc tgctgctggg taatgttccg ccacgtcatc
    10141 agaacaaaga cggttcggta gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc
    10201 cgactggcga acctgcggcg gcagcggaaa tgaccaaatg gtttaacacc aactatcact
    10261 acatggtgcc ggaattcgtt aaaggccaac agttcaaact gacctggacg cagctgctgg
    10321 aggaagtgga cgaggcgctg gcgctgggcc acaacgttaa gcctgtgctg ctggggccgg
    10381 ttacttacct gtggctgggg aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga
    10441 acgacattct gccggtttat cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt
    10501 gggtacagat tgatgaaccc gcgctggtac tggaactacc acaggcgtgg ctggacgcat
    10561 acaaacccgc ttacgacgcg ctccagggac aggtgaaact gctgctgacc acctattttg
    10621 aaggcgtaac gccaaatctc gacacgatta ctgcgctgcc tgttcagggt ctgcatgttg
    10681 acctcgtaca tggtaaagat gacgttgctg aactgcacaa gcgcctgcct tctgactggt
    10741 tgctgtctgc gggtctgatc aatggtcgta acgtctggcg cgccgatctt accgagaaat
    10801 atgcgcaaat taaggacatt gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt
    10861 tgctgcacag ccccatcgac ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct
    10921 ggtttgcctt cgccctacaa aaatgccatg aactggcact gctgcgcgat gcgctgaaca
    10981 gtggtgacac ggcagctctg gcagagtgga gcgccccgat tcaggcacgt cgtcactcta
    11041 cccgcgtaca taatccggcg gtagaaaagc gtctggcggc gatcaccgcc caggacagcc
    11101 agcgtgcgaa tgtctatgaa gtgcgtgccg aagcccagcg tgcgcgtttt aaactgccag
    11161 cgtggccgac caccacgatt ggttctttcc cgcaaaccac ggaaattcgt accctgcgtc
    11221 tggatttcaa aaagggcaat ctcgacgcca ataactaccg cacgggcatt gcggaacata
    11281 tcaggcaggc cattgttgaa caggaacgtt tgggactgga tgtgctggta catggcgagg
    11341 ccgagcgtaa tgacatggtg gaatactttg gcgagcacct cgacgggttt gtctttacgc
    11401 aaaacggttg ggtacagagc tacggttccc gctgcgtgaa gccaccgatt gttattggtg
    11461 acgttagtcg cccggcaccg attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg
    11521 acaaaccggt gaaagggatg ctgacggggc cggtgaccat actctgctgg tcgttcccgc
    11581 gtgaagatgt cagccgtgaa accatcgcca aacagattgc gctggcgctg cgtgatgagg
    11641 tggccgatct ggaagcggca gggattggta tcatccagat tgacgaaccg gcgctgcgcg
    11701 aaggtttacc actgcgtcgt agcgactggg acgcctatct ccagtggggc gtagaggcct
    11761 tccgtatcaa cgccgccgtg gcgaaagatg acacacaaat ccacactcac atgtgttatt
    11821 gcgagttcaa cgacatcatg gattccattg cggcgctgga tgcagacgtc atcaccatcg
    11881 aaacctcgcg ttccgacatg gagttgctgg agtcgttcga agagtttgat tatccgaatg
    11941 aaatcggtcc aggcgtctat gacattcact cgccaaacgt accgagcgtg gaatggattg
    12001 aagccctgct gaagaaagcg gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg
    12061 actgtggcct gaaaacgcgc ggctggccag aaacccgcgc ggcactggcg aacatggtgc
    12121 aggcggcgca gaatttgcgt cgggggtaaa atccaaaccg ggtggtaata ccacccggtc
    12181 ttttctcatt acagcgactt cttcccacca tactgcttaa accattccag catacgctgc
    12241 cagccatctt tcgcagattc ggcatgatag ctcgggcgat aatcagcgtt gaatgcatgc
    12301 ccggcatccg ggtagacgat aatctctgct ttcgcattag cagcccgcag cgcctggcgc
    12361 atggtttcaa cgctctcctg cggaatactg ttatccagac cgccatataa gccgagaacc
    12421 ggcgcgttaa gatcggttgc gatatcaaca ggttgtttcg gtgaattcag cgacttatcg
    12481 cccgtcagtt tgccgtacca cgccactgcg gcttttagct gtggattatg cgcggcatac
    12541 agccaggtga tacgtccacc ccagcagaat ccggtgatca ttaaacgatg aacatcgccg
    12601 ccgttgcggg aagcccaact ggcgacatga tcgagatcgg ccagcacctg cgagtcaggc
    12661 acttttgcta ccagaccgct aagcaacgtg gggatatcgg caaaatcatt cggatcgcct
    12721 tcgcggaagt aaagttcagg tgcgatagcc agatacccct ccagcgccag acggcgacaa
    12781 atgtcgcgga tatgttcatg cacgccaaaa atttcctgca ctacaatgac cactggcagt
    12841 tggccatcgc tttgctttgg tctggcatgg taagcaggca tgttatcccc ttgtgaaggg
    12901 atagaggtga agcccgccac aatcgcgtcg tccggggtct gaacgatggt cgaagcgaga
    12961 ggcgatgcag caggtgtaaa tccagattgt tgtgttgttg ccatggtatt ctccgtaccc
    13021 ttataaaaat gttgcgcaat gttaactata gtcagcatgc aacaaatcac attgcctgaa
    13081 tcggctcatc ttttatgcag tcctgcagaa tgaagagtga tttatgtgat ttgaatcact
    13141 tttggtgggt aaatttatgc aacgcatttg cgccatggtg atgagtatca cgaaaaaatg
    13201 ttaaaccctt cggtaaagtg tctttttgct tcttctgact aaaccgattc acagaggagt
    13261 tgtatatgtc caagtctgat gtttttcatc tcggcctcac taaaaacgat ttacaagggg
    13321 ctacgcttgc catcgtccct ggcgacccgg atcgtgtgga aaagatcgcc gcgctgatgg
    13381 ataagccggt taagctggca tctcaccgcg aattcactac ctggcgtgca gagctggatg
    13441 gtaaacctgt tatcgtctgc tctaccggta tcggcggccc gtctacctct attgctgttg
    13501 aagagctggc acagctgggc attcgcacct tcctgcgtat cggtacaacg ggcgctattc
    13561 agccgcatat taatgtgggt gatgtcctgg ttaccacggc gtctgtccgt ctggatggcg
    13621 cgagcctgca cttcgcaccg ctggaattcc cggctgtcgc tgatttcgaa tgtacgactg
    13681 cgctggttga agctgcgaaa tccattggcg cgacaactca cgttggcgtg acagcttctt
    13741 ctgatacctt ctacccaggc caggaacgtt acgatactta ctctggtcgc gtagttcgtc
    13801 actttaaagg ttctatggaa gagtggcagg cgatgggcgt aatgaactat gaaatggaat
    13861 ctgcaaccct gctgaccatg tgtgcaagtc agggcctgcg tgccggtatg gtagcgggtg
    13921 ttatcgttaa ccgcacccag caagagatcc cgaatgctga gacgatgaaa caaactgaaa
    13981 gccaagcggt gaaaatcgtg gtggaagcgg cgcgtcgtct gctgtaattc tcttctcctg
    14041 tctgaaggcc gacgcgttcg gccttttgta tttttgcgta gcgcctcgca ggaaatgcct
    14101 ttccaactgg acgtttgtac agcacaattc tattttgtgc gggtaagttg ttgcgtcagg
    14161 aggcgttgtg gatttctcaa tcatggttta cgcagttatt gcgttggtgg gtgtggcaat
    14221 tggctggctg tttgccagtt atcaatatgc gcagcaaaaa gccgagcaat tagctgaacg
    14281 tgaagagatg gtcgcggagt taagcgcggc aaaacaacaa attacccaaa gcgagcactg
    14341 gcgtgcagag tgcgagttac tcaataacga agtgcgcagc ctgcaaagta ttaacacctc
    14401 tctggaggcc gatctgcgtg aagtaaccac gcggatggaa gccgcacagc aacatgctga
    14461 cgataaaatt cgccagatga ttaacagcga gcagcgcctc agtgagcagt ttgaaaacct
    14521 cgccaaccgt atttttgagc acagcaatcg ccgggttgat gagcaaaacc gtcagagtct
    14581 gaacagcctg ttgtcgccgc tacgtgaaca actggacggt ttccgccgtc aggttcagga
    14641 cagcttcggt aaagaagcac aagaacgcca taccctgacc cacgaaattc gcaatctcca
    14701 gcaactcaac gcgcaaatgg cccaggaagc gatcaacctg acgcgcgcgc tgaaaggcga
    14761 caataaaacc cagggcaact ggggcgaggt agtattgacg cgggtgctgg aggcttccgg
    14821 tctgcgtgaa gggtatgaat atgaaaccca ggtcagcatc gaaaatgacg cccgctcgcg
    14881 gatgcagccg gatgtcatcg tgcggctgcc gcagggaaaa gatgtggtga tcgacgccaa
    14941 aatgacgctg gtcgcctatg aacgctattt taacgccgaa gacgactaca cccgcgaaag
    15001 cgcgctacag gaacatatcg cgtcggtgcg taaccatatc cgtttgctgg gacgcaaaga
    15061 ttatcaacag ctgccggggc tgcgaactct ggattacgtg ctgatgttta ttcccgttga
    15121 acccgctttt tcactggcgc ttgaccgcca gccggagctg atcaccgaag cgttgaaaaa
    15181 caatatcatg ctggttagcc cgactacgct gctggtggcg ttgcgtacta ttgccaacct
    15241 gtggcgttat gagcatcaaa gccgcaacgc ccagcaaatc gccgatcgtg ccagcaagct
    15301 gtacgacaag atgcgtttgt tcatcgatga catgtccgcg attggtcaaa gtctcgacaa
    15361 agcgcaggat aactaccgcc aggcaatgaa aaaactctct tcaggtcgcg gaaatgtgct
    15421 ggcgcaggca gaagcgtttc gcggtttagg agtagaaatt aaacgcgaga ttaatccgga
    15481 tttggctgaa caggcggtga gccaggatga agagtatcga cttcggtcgg ttccggagca
    15541 gccgaatgat gaagcttatc aacgcgatga tgaatataat cagcagtcgc gctagcccat
    15601 taggggtagt taagccgggt agaaatctag ggcatcgacg cccaatctgt tacacttctg
    15661 gaacaatttt ttgatgagca ggcattgaga tggtggataa gtcacaagaa acgacgcact
    15721 ttggttttca gaccgtcgcg aaggaacaaa aagcggatat ggtcgcccac gttttccatt
    15781 ccgtggcatc aaaatacgat gtcatgaatg atttgatgtc atttggtatt catcgtttgt
    15841 ggaagcgatt cacgattgat tgcagcggcg tacgccgtgg gcagaccgtg ctggatctgg
    15901 ctggtggcac cggcgacctg acagcgaaat tctcccgcct ggtcggagaa actggcaaag
    15961 tggtccttgc tgatatcaat gaatccatgc tcaaaatggg acgcgagaag ctgcgtaata
    16021 tcggtgtgat tggcaacgtt gagtatgttc aggcgaacgc tgaggcgctg ccgttcccgg
    16081 ataacacctt tgattgcatc accatttcgt ttggtctgcg taacgtcacc gacaaagata
    16141 aagcactgcg ttcaatgtat cgcgtgctga aacccggcgg ccgcctgctg gtgcttgagt
    16201 tctcgaagcc aattatcgag ccgctgagca aagcctatga tgcatactcc ttccatgtgc
    16261 tgccgcgtat tggctcactg gtcgcgaacg acgccgacag ctaccgttat ctggcagaat
    16321 ccatccgtat gcatcccgat caggataccc tgaaagccat gatgcaggat gccggattcg
    16381 aaagtgtcga ctactacaat ctgacggcgg gggttgtggc gctgcatcgt ggttataagt
    16441 tctgacagga gaccggaaat gccttttaaa cctttagtga cggcaggaat tgaaagtctg
    16501 ctcaacacct tcctgtatcg ctcacccgcg ctgaaaacag cccgctcgcg tctgctgggt
    16561 aaagtattgc gcgtggaggt aaaaggcttt tcgacgtcat tgattctggt gttcagcgaa
    16621 cgccaggttg atgtactggg cgaatgggca ggcgatgctg actgcaccgt tatcgcctac
    16681 gccagtgtgt tgccgaaact tcgcgatcgc cagcagctta ccgcactgat tcgcagtggt
    16741 gagctggaag tgcagggcga tattcaggtg gtgcaaaact tcgttgcgct ggcagatctg
    16801 gcagagttcg accctgcgga actgctggcc ccttataccg gtgatatcgc cgctgaagga
    16861 atcagcaaag ccatgcgcgg aggcgcaaag ttcctgcatc acggcattaa gcgccagcaa
    16921 cgttatgtgg cggaagccat tactgaagag tggcgtatgg cacccggtcc gcttgaagtg
    16981 gcctggtttg cggaagagac ggctgccgtc gagcgtgctg ttgatgccct gaccaaacgg
    17041 ctggaaaaac tggaggctaa atgacgccag gtgaagtacg gcgcctatat ttcatcattc
    17101 gcactttttt aagctacgga cttgatgaac tgatccccaa aatgcgtatc accctgccgc
    17161 tacggctatg gcgatactca ttattctgga tgccaaatcg gcataaagac aaacttttag
    17221 gtgagcgact acgactggcc ctgcaagaac tggggccggt ttggatcaag ttcgggcaaa
    17281 tgttatcaac ccgccgcgat ctttttccac cgcatattgc cgatcagctg gcgttattgc
    17341 aggacaaagt tgctccgttt gatggcaagc tggcgaagca gcagattgaa gctgcaatgg
    17401 gcggcttgcc ggtagaagcg tggtttgacg attttgaaat caagccgctg gcttctgctt
    17461 ctatcgccca ggttcatacc gcgcgattga aatcgaatgg taaagaggtg gtgattaaag
    17521 tcatccgccc ggatattttg ccggtcatta aagcggatct gaaacttatc taccgtctgg
    17581 ctcgctgggt gccgcgtttg ctgccggatg gtcgccgtct gcgcccaacc gaagtggtgc
    17641 gcgagtacga aaagaccttg attgatgaac tgaatttgct gcgggaatct gccaacgcca
    17701 ttcagcttcg gcgcaatttt gaagacagcc cgatgctcta catcccggaa gtttaccctg
    17761 actattgtag tgaagggatg atggtgatgg agcgcattta cggcattccg gtgtctgatg
    17821 ttgcggcgct ggagaaaaac ggcactaaca tgaaattgct ggcggaacgc ggcgtgcagg
    17881 tgttcttcac tcaggtcttt cgcgacagct ttttccatgc cgatatgcac cctggcaaca
    17941 tcttcgtaag ctatgaacac ccggaaaacc cgaaatatat cggcattgat tgcgggattg
    18001 ttggctcgct aaacaaagaa gataaacgct atctggcaga aaactttatc gccttcttta
    18061 atcgcgacta tcgcaaagtg gcagagctac acgtcgattc aggctgggtg ccaccagata
    18121 ccaacgttga agagttcgaa tttgccattc gtacggtctg tgaacctatc tttgagaaac
    18181 cgctggccga aatttcgttt ggacatgtac tgttaaatct gtttaatacg gcgcgtcgct
    18241 tcaatatgga agtgcagccg caactggtgt tactccagaa aaccctgctc tatgtcgaag
    18301 gggtaggacg ccagctttat ccgcagctcg atttatggaa aacagcgaag cctttcctgg
    18361 agtcgtggat taaagatcag gtcggtattc ctgcgctggt gagagcattt aaagaaaaag
    18421 cgccgttctg ggtcgaaaaa atgccagaac tgcctgaatt ggtttacgac agtttgcgcc
    18481 agggcaagta tttacagcac agtgttgata agattgcccg cgagcttcag tcaaatcatg
    18541 tacgtcaggg acaatcgcgt tattttctcg gaattggcgc tacgttagta ttaagtggca
    18601 cattcttgtt ggtcagccga cctgagtggg ggctgatgcc cggctggtta atggcaggtg
    18661 gtctgatcgc ctggtttgtc ggttggcgca aaacacgctg attttttcat cgctcaaggc
    18721 gggccgtgta acgtataatg cggctttgtt taatcatcat ctaccacaga ggaacatgta
    18781 tgggtggtat cagtatttgg cagttattga ttattgccgt catcgttgta ctgctttttg
    18841 gcaccaaaaa gctcggctcc atcggttccg atcttggtgc gtcgatcaaa ggctttaaaa
    18901 aagcaatgag cgatgatgaa ccaaagcagg ataaaaccag tcaggatgct gattttactg
    18961 cgaaaactat cgccgataag caggcggata cgaatcagga acaggctaaa acagaagacg
    19021 cgaagcgcca cgataaagag caggtgtaat ccgtgtttga tatcggtttt agcgaactgc
    19081 tattggtgtt catcatcggc ctcgtcgttc tggggccgca acgactgcct gtggcggtaa
    19141 aaacggtagc gggctggatt cgcgcgttgc gttcactggc gacaacggtg cagaacgaac
    19201 tgacccagga gttaaaactc caggagtttc aggacagtct gaaaaaggtt gaaaaggcga
    19261 gcctcactaa cctgacgccc gaactgaaag cgtcgatgga tgaactacgc caggccgcgg
    19321 agtcgatgaa gcgttcctac gttgcaaacg atcctgaaaa ggcgagcgat gaagcgcaca
    19381 ccatccataa cccggtggtg aaagataatg aagctgcgca tgagggcgta acgcctgccg
    19441 ctgcacaaac gcaggccagt tcgccggaac agaagccaga aaccacgcca gagccggtgg
    19501 taaaacctgc tgcggacgct gaaccgaaaa ccgctgcacc ttccccttcg tcgagtgata
    19561 aaccgtaaac atgtctgtag aagatactca accgcttatc acgcatctga ttgagctgcg
    19621 taagcgtctg ctgaactgca ttatcgcggt gatcgtgata ttcctgtgtc tggtctattt
    19681 cgccaatgac atctatcacc tggtatccgc gccattgatc aagcagttgc cgcaaggttc
    19741 aacgatgatc gccaccgacg tggcctcgcc gttctttacg ccgatcaagc tgacctttat
    19801 ggtgtcgctg attctgtcag cgccggtgat tctctatcag gtgtgggcat ttatcgcccc
    19861 agcgctgtat aagcatgaac gtcgcctggt ggtgccgctg ctggtttcca gctctctgct
    19921 gttttatatc ggcatggcat tcgcctactt tgtggtcttt ccgctggcat ttggcttcct
    19981 tgccaatacc gcgccggaag gggtgcaggt atccaccgac atcgccagct atttaagctt
    20041 cgttatggcg ctgtttatgg cgtttggtgt ctcctttgaa gtgccggtag caattgtgct
    20101 gctgtgctgg atggggatta cctcgccaga agacttacgc aaaaaacgcc cgtatgtgct
    20161 ggttggtgca ttcgttgtcg ggatgttgct gacgccgccg gatgtcttct cgcaaacgct
    20221 gttggcgatc ccgatgtact gtctgtttga aatcggtgtc ttcttctcac gcttttacgt
    20281 tggtaaaggg cgaaatcggg aagaggaaaa cgacgctgaa gcagaaagcg aaaaaactga
    20341 agaataaatt caaccgcccg tcagggcggt tgtcatatgg agtacaggat gtttgatatc
    20401 ggcgttaatt tgaccagttc gcaatttgcg aaagaccgtg atgatgttgt agcgcgcgct
    20461 tttgacgcgg gagttaatgg gctactcatc accggcacta acctgcgtga aagccagcag
    20521 gcgcaaaagc tggcgcgtca gtattcgtcc tgttggtcaa cggcgggcgt acatcctcac
    20581 gacagcagcc agtggcaagc tgcgactgaa gaagcgatta ttgagctggc cgcgcagcca
    20641 gaagtggtgg cgattggtga atgtggtctc gactttaacc gcaacttttc gacgccggaa
    20701 gagcaggaac gcgcttttgt tgctcagcta cgcattgccg cagaattaaa catgccggta
    20761 tttatgcact gtcgcgatgc ccacgagcgg tttatgacat tgctggagcc gtggctggat
    20821 aaactgcctg gtgcggttct tcattgcttt accggcacac gcgaagagat gcaggcgtgc
    20881 gtggcgcgtg gaatttatat cggcattacc ggttgggttt gcgatgaacg acgcgggctg
    20941 gagctgcggg aattgttgcc gttgattccg gcggagaaat tgctgatcga aactgatgcg
    21001 ccgtatctgc tcccgcgcga tctcacgcca aagccatcat cccggcgcaa cgagccagcc
    21061 catctgcccc atattttgca acgtattgcg cactggcgtg gagaagatgc cgcatggctg
    21121 gctgccacca cggatgccaa tgtcaaaaca ctgtttggga ttgcgtttta gagtttgcgg
    21181 aactcggtgt tcttcacact gtgcttaatc tctttattaa taagattaag caatagcatg
    21241 gagcgagcct caccatcggg ttcggtgaaa atggcctgaa agccttcgaa cgcgccttcg
    21301 gtaataatca ccttatcacc cggataaggg gttgccggat cgacaatgtc tttcggttta
    21361 tataccgata gctgatgaat caccgccgat gggactatcg ctggcgacgc gccaaagcgc
    21421 acgaagtggc tgacaccgcg ggtcgcgttg atagtcgtgg tatgaatcac ttctggatca
    21481 aattcgacaa acaggtagtt gggaaacaat ggctcactga ctgcagtacg ttttccacgc
    21541 acgatttttt ccagggtgat catcggtgcc aggcaattca cagcctgtct ttcgaggtgt
    21601 tcctgggcac gttgaagttg cccgcgcttg cagtacagta aataccagga ttgcataatg
    21661 actcttatcc gtttaatcgg ggcgcaagga tagcaaaagc tttacgctaa gttaattata
    21721 ttccccggtt tgcgttatac cgtcagagtt cacgctaatt taacaaattt acagcatcgc
    21781 aaagatgaac gccgtataat gggcgtagat taagaggcta caatggacgc catgaaatat
    21841 aacgatttac gcgacttctt gacgctgctt gaacagcagg gtgagctaaa acgtatcacg
    21901 ctcccggtgg atccgcatct ggaaatcact gaaattgctg accgcacttt gcgtgccggt
    21961 gggcctgcgc tgttgttcga aaaccctaaa ggctactcaa tgccggtgct gtgcaacctg
    22021 ttcggtacgc caaagcgcgt ggcgatgggc atggggcagg aagatgtttc ggcgctgcgt
    22081 gaagttggta aattattggc gtttctgaaa gagccggagc cgccaaaagg tttccgcgac
    22141 ctgtttgata aactgccgca gtttaagcaa gtattgaaca tgccgacaaa gcggctgcgt
    22201 ggtgcgccct gccaacaaaa aatcgtctct ggcgatgacg tcgatctcaa tcgcattccc
    22261 attatgacct gctggccgga agatgccgcg ccgctgatta cctgggggct gacagtgacg
    22321 cgcggcccgc ataaagagcg gcagaatctg ggcatttatc gccagcagct gattggtaaa
    22381 aacaaactga ttatgcgctg gctgtcgcat cgcggcggcg cgctggatta tcaggagtgg
    22441 tgtgcggcgc atccgggcga acgtttcccg gtttctgtgg cgctgggtgc cgatcccgcc
    22501 acgattctcg gtgcagtcac ccccgttccg gatacacttt cagagtatgc gtttgccgga
    22561 ttgctgcgcg gtaccaagac cgaagtggtg aagtgtatct ccaatgacct tgaagtgccc
    22621 gccagtgcgg agattgtgct ggaagggtat atcgaacaag gtgaaacggc gccggaaggg
    22681 ccgtatggcg accacaccgg ttactataac gaagtcgata gtttcccggt atttaccgtg
    22741 acgcatatta cccagcgtga agatgcgatt taccattcca cctataccgg gcgtccgcca
    22801 gatgagcccg cggtactggg cgtggcgttg aacgaagtgt ttgtaccgat tctgcaaaaa
    22861 cagttcccgg aaattgtcga tttttacctg ccgccggaag gctgttctta tcgtctggcg
    22921 gtagtgacga tcaaaaaaca gtacgccgga cacgcgaagc gcgtcatgat gggcgtctgg
    22981 tcgttcttac gccagtttat gtacactaaa tttgtgatcg tttgcgatga tgacgtcaac
    23041 gcacgcgact ggaacgatgt gatttgggcg attaccaccc gtatggaccc ggcgcgggat
    23101 actgttctgg tagaaaatac gcctattgat tatctggatt ttgcctcgcc tgtctccggg
    23161 ctgggttcaa aaatggggct ggatgccacg aataaatggc cgggggaaac ccagcgtgaa
    23221 tggggacgtc ccatcaaaaa agatccagat gttgtcgcgc atattgacgc catctgggat
    23281 gaactggcta tttttaacaa cggtaaaagc gcctgatgcg cgtttgtttt gccctattta
    23341 tcgatccgac agagaaagcg catgacaacc ttaagctgta aagtgacctc ggtagaagct
    23401 atcacggata ccgtatatcg tgtccgcatc gtgccagacg cggccttttc ttttcgtgct
    23461 ggtcagtatt tgatggtagt gatggatgag cgcgacaaac gtccgttctc aatggcctcg
    23521 acgccggatg aaaaagggtt tatcgagctg catattggcg cttctgaaat caacctttac
    23581 gcgaaagcag tcatggaccg catcctcaaa gatcatcaaa tcgtggtcga tattccccac
    23641 ggagaagcgt ggctgcgcga tgatgaagag cgtccgatga ttttgattgc gggcggcacc
    23701 gggttctctt atgcccgctc gattttgctg acagcgttgg cgcgtaaccc aaaccgtgat
    23761 atcaccattt actggggcgg gcgtgaagag cagcatctgt atgatctctg cgagcttgag
    23821 gcgctttcgt taaagcaccc tggtctgcaa gtggtgccgg tggttgaaca accggaagca
    23881 ggctggcgtg ggcgtactgg taccgtgtta acggcggtat tgcaggatca tggtacgctg
    23941 gcagagcatg atatctatat tgccggacgt tttgagatgg cgaaaattgc ccgtgacctg
    24001 ttttgcagtg agcgtaatgc gcgggaagat cgcctgtttg gcgatgcgtt tgcatttatc
    24061 tgagatataa aaaaacccgc ccctgacagg cgggaagaac ggcaactaaa actttattca
    24121 gtggcattta gatctatgtc gcatccggca attggtgcac gacgcctgat gcgacgctgg
    24181 cgcgtcttat caggcctaca ttggtgccgg atcggtagac cggataaggc gttcacgccg
    24241 catccggcaa gtggttaaac ccgctcaaac accgtcgcaa taccctgacc cagaccgata
    24301 cacatcgtcg ccagaccaaa ctgaacgtct ttgcgttcca tcagattcag cagcgtggtg
    24361 ctgatacgcg caccggaaca gcccagcgga tgacccagcg cgatcgcacc accattgagg
    24421 ttgatcttct cgtcaatctg ctccattagc ccgagatctt taatgcacgg caggatctgc
    24481 gcggcaaagg cttcgttcat ctcaaacacg ccgatatcgc tggcagaaag ccccgctttt
    24541 ttcagcgcca gcttcgaggc cggaaccggg ccgtaaccca taatcgatgg gtcacaacca
    24601 acgaccgcca tcgaacgcac acgagcgcgc ggcttaagac ctaattcatg ggcgcggctt
    24661 tcactcatca ccagcatggc agctgcgcca tcggaaagtg cagaagatgt gcccgccgtt
    24721 accgtaccgt ttactggatc aaacgccgga cgcagcgtgg cgagggcttc cacggtggtt
    24781 tccgggcgaa tcacttcgtc gtaattaaac tgcttcagga cgccgtcggc atcgtgacca
    24841 ccagttggga tgatttcatt tttaaatgcg gccgactgcg tggcggccca ggcgcgggcg
    24901 tgtgaccgcg cggcaaaggc atcctgcatt tcacggctga taccgtgcat acgcgccagc
    24961 atttctgccg ttaagcccat catgcccgcc gctttggcga cattgcggct caggccgggg
    25021 tgaaaatcga cgccgtgact catcggcaca tggcccatat gctccacgcc gccaaccaga
    25081 catgcctgcg catcgccagt catgatcatt cgtgctgcgt catgcagtgc ctgcatggat
    25141 gaaccacaca agcgattgac ggtaaccgcc gggacagagt gcggcacttc tgccagcagc
    25201 gccgcgttac gggcgatatt aaaaccctgc tccagcgtct gctgcacaca accccagtaa
    25261 atatcgtcga gggccgccgc ttccagcgcc gggttacgcg ccagcaggct acgcattaaa
    25321 tgagcggaga gatcttctgc acgcacgtta cgaaaagcac cgcccttcga acggcccatc
    25381 ggggtgcgaa ttgcatcgac aatgacaacc tgttccattg tgactcctta agccgttttc
    25441 aggtcgccaa ccggacgggc tggctcaacc ggaggatagt acggttcgtt atgacgcgct
    25501 ttattacgca gacctttcgg cacttcatac agcgggccga ggtgctgata ttgctgtgcc
    25561 atatcgaggt attttgcgct accgagggtg tccagccagc ggaacgcgcc gccgtggaac
    25621 ggagggaagc ccaggccgta gaccagcgcc atatccgctt ccgccggagt ggcgataatg
    25681 ccttcctcca gacagcgcac cacttcgttg accatcggga tcatcatgcg ggcgataatc
    25741 tcttcttcgc tgaaatcgcg cttcggctgg ctcacttctg ccagcaggtc ttcaacggcg
    25801 gcgtcttctt ctttcttcgg cttacctttg ctgtcttctt tataacgcca gaaaccgagg
    25861 ccgttcttct gaccaaagcg gttggcatca aacagcgcgt cgatggcatc gcggtaatct
    25921 ttctgcatcc gctgcgggaa gcctgctgcc atgacagcct gagcgtgatg cgcggtatca
    25981 atgcccacaa cgtccagcag atatgccggg cccatcggcc agccaaactg tttttccatc
    26041 actttgtcga tcttgcggaa atccgcgccg tcgcgcagca gctggctgaa accggcgaaa
    26101 tacgggaaca gcacgcggtt aacaaagaag ccggggcagt cgttaaccac aatcggcgtc
    26161 ttgcccatct tgctcgccca ggcgacaact ttcgcgatgg tttcgtcgga gcttttctcg
    26221 ccgcgaataa tttctaccaa cggcattcgg tggaccgggt taaagaagtg catcccgcag
    26281 aagttttccg ggcgttccag cgcgttggcc agttcgctga taggaatggt tgaagtgtta
    26341 gacgccagca cggtatcctg gcgtactttt tgttcggttt ctgccagtac ggcttttttc
    26401 actttcgggt tttcaacaac cgcttctacc acaatatcca cgcggtcaaa tccggcgtag
    26461 tcgagcgttg ggtggattgt ggagatcacg ccagccagtt tcagaccatc gatcttgccg
    26521 cgctcaagct gcttgttcag cagtttcgcg gcttcggtca tgccgagggt taacgacttg
    26581 tcgttgatat ctttcatgac aaccggcacg cctttccacg cagactggta agcgatgccg
    26641 ccgcccataa tgcctgcacc cagcaccgcg gcctgtttcg gggtttcaac gtctttggtg
    26701 agtttcttcg ctttgccttt tacatattga tcgttaagga aaatgccgac cagtgcgcgg
    26761 gcttcgttgg tatgcgccag cgggacaaaa cttttgtttt ccaggtttaa ggcttcttca
    26821 cgaccaaaac gggccgcagc ttcaatggtt tttactgcgg tgatgggggc cggataatgt
    26881 ttccccgctg tttgtgcgac catcccttta gcgatggtga agctcatggt ggcttcaatc
    26941 ttgctcagtt ttagtggttc cagcttcggc tgacgttttg ctttccagtc gaggtcgccg
    27001 ttaatggcct ggcgtaaaac cgcctttgcg ccttcaacca gtttttctgc tttgactacg
    27061 ccatccacca gaccgatttt cagcgcctga tccgcgccga catctttacc ggcggcaatg
    27121 atttccagcg cactgtcagc gcccagcata cgtggcatac gtacagaacc gccaaagccc
    27181 ggcatgatgc ccagtttggt ttccggcaga ccgatgcgca gatccggcgt cgccagacga
    27241 taatcggtcg ccagcacgca ttcgcagcca ccgcccagcg catagccgtt gacggcagca
    27301 atggtcggca ccggcagatc ttccaggcga ttaaacacgc tattggcaaa gtgcagccac
    27361 tgacttaact gttcttcagg aacgaggaac agggacaaaa attcggtgat atcagcaccg
    27421 acgataaagg ctgctttgtt cgaacgcagc agcagccctt ttagatctga ttgctgttcc
    27481 agcacgccga tggcctcgcc gaggctggcg acggtcgcag tgtcgagttt attaactgaa
    27541 cctggggcat caaataccag ttcggcaatg ccatcttcca gccagtcaag gtacagggtg
    27601 tcgcctttgt aaagcatgtc agtctcctga atccgcaagg tgatctggtc gtaccagatg
    27661 catcgaagtg tgtattttgt gttaaaaata tgcaaataaa agattaaaga aatgccgatc
    27721 tgatcacgct cggcagaaat cacgctctgg atgaacgatg tgctaagatg cggagactta
    27781 aggtcaaaaa aacagaaggg taaaaaatgg aatcactggc ctcactctat aaaaatcata
    27841 tagctacctt acaggaacgg actcgcgatg cgctggcgcg cttcaagctg gatgcgttac
    27901 ttattcactc cggcgaactg ttcaacgttt ttctcgacga tcatccctat ccgtttaaag
    27961 tgaacccgca attcaaagcg tgggtgccgg taactcaggt gccaaactgc tggctgctgg
    28021 tggatggcgt gaacaagccg aaactgtggt tctatctgcc ggttgattac tggcacaacg
    28081 tcgaaccgct gccgacctct ttctggactg aagacgtgga agtgatcgcg ctgccgaaag
    28141 ccgatggcat tggtagcctg ctgcctgctg cgcgcgggaa tatcggttat atcggtccgg
    28201 tgccggaacg tgcgctgcaa ctgggtattg aggccagcaa tatcaacccg aaaggggtga
    28261 tcgactacct gcattactac cgctccttca aaaccgagta cgaactggcc tgtatgcgtg
    28321 aagcgcagaa aatggcggtc aacggtcatc gcgcggcaga agaagcgttc cgttctggca
    28381 tgagcgagtt cgatatcaac attgcctatc tgaccgcgac cggtcatcgt gataccgacg
    28441 taccttacag caacattgtg gcgctcaacg aacacgctgc ggtgttgcat tacaccaaac
    28501 tggaccacca ggcaccggaa gagatgcgca gcttcctgct ggatgccggg gccgaatata
    28561 acggctatgc ggctgacctg acccgtacct ggtcggcaaa aagtgacaac gactacgcac
    28621 agctggtgaa agacgtaaat gatgaacaac tggcgctgat cgctaccatg aaagcaggcg
    28681 tcagctatgt ggattaccac atccagttcc atcagcgcat cgccaaactg ctgcgtaaac
    28741 atcaaatcat caccgatatg agtgaagaag cgatggtcga aaacgatctc accggaccgt
    28801 ttatgccgca cggtattggc catccgctgg gcctgcaggt gcatgacgta gccggtttta
    28861 tgcaggatga tagcggtacg cacctcgcgg caccggcaaa atatccgtac ctgcgctgca
    28921 cccgtattct ccagccgggc atggtgttaa ccatcgaacc gggtatctac ttcattgaat
    28981 cgctgctggc accgtggcgt gaagggcagt tcagcaagca cttcaactgg cagaaaattg
    29041 aagcactgaa accgttcggc ggcattcgta tcgaagacaa cgtggtgatc cacgaaaaca
    29101 acgtggaaaa catgacccgg gatctgaaac tggcgtgatg gaaagctggt taattcctgc
    29161 ggcaccggtc acggtcgttg aagagatcaa aaaaagccgc ttcataacga tgttggcgca
    29221 taccgatggc gttgaggcgg cgaaagcgtt tgttgaatcg gtgcgggcag aacaccccga
    29281 tgcccgccac cattgcgtgg cgtgggtggc gggtgcgccg gatgattctc aacagctggg
    29341 tttctctgac gacggggagc cggcgggaac ggcaggtaaa ccgatgctcg cccagctaat
    29401 gggcagcggc gtcggggaaa ttaccgctgt ggtagtgcgc tactacggcg gcatattgct
    29461 tggcaccggt gggttagtga aagcgtatgg cggcggcgtg aatcaggcgc tgcgccagct
    29521 aacgacccaa cgcaagacgc cattaaccga atatactttg caatgtgaat atagtcagtt
    29581 aaccggcatt gaagcgttgc tggggcagtg tgacggcaaa attatcaaca gtgattatca
    29641 ggcattcgtt ctgctgcggg tggcgcttcc ggcggcgaaa gtggctgaat tttccgcaaa
    29701 gctggcggat tttagccgtg gttcattgca attgttagcg attgaagaat aatccccact
    29761 tcgttttgca gaactaagga agcggcagag atgcattttc gcgccattac ccgaatcgtt
    29821 ggactactgg tcatcttatt ttcagggacc atgattatcc ctgggctggt agcactcatc
    29881 taccgggatg gagcgggccg cgcttttacc cagacctttt ttgtcgccct cgccattggt
    29941 tctatgctgt ggtggccgaa ccgtaaagag aagggcgaac tgaaatcccg tgaggggttt
    30001 ctgatagtgg tgctgttctg gaccgtgctg ggtagtgtcg gtgcgctccc ttttatcttc
    30061 tcggaaagcc cgaacctcac gattaccgat gcgttttttg aatctttctc tggcctgacc
    30121 accaccgggg ccactacgct ggtggggctg gattcgctcc ctcatgccat cctcttttat
    30181 cgccagatgc tgcaatggtt tggcgggatg gggatcatcg tgttagcggt tgcgatactg
    30241 cctatcctcg gcgtgggtgg gatgcagctc tatcgcgcag aaatgcccgg cccgctgaaa
    30301 gataacaaaa tgcgcccgcg aattgcggaa acggcgaaaa ccctgtggtt gatttatgtc
    30361 ttgctgaccg tcgcctgtgc gctggcgttg tggtttgccg gaatggatgc ctttgatgcc
    30421 atcggccata gctttgcgac tatcgctatt ggcggcttct cgacacatga tgccagtatc
    30481 ggttatttcg acagcccgac tattaacact atcattgcta tcttcctgct gatctccggc
    30541 tgtaactacg gtctgcactt ttcactgtta agtgggcgta gtctgaaggt ttattggcgc
    30601 gatccggaat ttcgcatgtt tatcggcgta cagtttacgc tggtggttat ttgtacactc
    30661 gtactgtggt ttcataatgt ctacagttcg gcgctgatga caattaacca ggcgtttttc
    30721 caggtggtat cgatggcgac aaccgccggg tttacgactg acagcattgc ccgctggccg
    30781 ctctttttgc cggtactgct tttatgttca gcttttatcg gcggttgcgc cgggtcaacg
    30841 ggcggtggcc tgaaagtgat ccgcatcctg ctgctgttta agcaggggaa ccgtgagctg
    30901 aaacgactgg tgcatccgaa cgccgtctat agcattaagc tggggaatcg cgcactgccg
    30961 gaacgtatcc tcgaagccgt ttggggattt ttctccgcct atgcattggt gtttattgtc
    31021 agtatgctgg cgattatcgc cacgggcgtg gatgactttt ctgcctttgc ctcagttgtt
    31081 gcgacattga ataacctggg gccagggctt ggcgtggttg ctgataactt taccagtatg
    31141 aacccggtgg ctaaatggat cctgattgcc aacatgctgt ttggtcgtct cgaggtcttt
    31201 acattgctgg tgctctttac cccgactttc tggcgtgaat gatggagtaa tacgtgaaaa
    31261 cattaattct tttctcaaca agggacggac aaacgcgcga gattgcctcc tacctggctt
    31321 cggaactgaa agaactgggg atccagacgg atgtcgccaa tgtgcaccgc attgaagaac
    31381 cacaatggga aaattatgac cgtgtggtca ttggtgcttc tattcgctat ggtcactacc
    31441 actctgcgtt ccaggaattt gtcaaaaaac atgcgacgcg actgaattcg atgccgagcg
    31501 ccttttattc cgtaaacctg gtggcgcgca aaccggagaa gcgtactcca cagaccaaca
    31561 gctacgcgcg gaagtttctg atgaactcgc aatggcgtcc cgatcgctgc gcggtcattg
    31621 ccggggcgct gcgttaccca cgttatcgct ggtacgaccg ttttatgatc aagctgatta
    31681 tgaagatgtc aggcggtgaa acggatacgc gcaaagaagt tgtctatacc gattgggagc
    31741 aggtggcgaa tttcgcccga gaaatcgccc atttaaccga caaaccgacg ctgaaataag
    31801 cacaaagaat aaaaaatgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg
    31861 aataactccc tataatgcgc caccactgac acggaacaac ggcaaacacg ttgccgggtc
    31921 agcggggttc tcctgagaat ctcaacagag aaaagcaaaa aatgcttgac tctgtagcgg
    31981 gaaagcgtat tatgca
//
LOCUS       DAZGXZ010000049        31734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000049 DAZGXZ010000000
VERSION     DAZGXZ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.049"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(238..810)
                     /locus_tag="ACHKJF_002350"
     CDS             complement(238..810)
                     /locus_tag="ACHKJF_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949698.1"
                     /translation="MLTKKMLLVCASCVALSACSAQEIAELNRQISDGAFAVTKVLNV
                     GVNQADDDLKYLSQAPRAKREKRDHVIEVPVDVDTAAGRLKRYYKFYSNEEVDAIRNN
                     GHANGKWAAAAISQNGHMWDAMPGSYYKMGSDWDGGEFDDHLTLELEKNGKGSRIYIT
                     YASPAPGHLKDKFIEPLVKRVKDVAEGRVR"
     gene            1002..1177
                     /locus_tag="ACHKJF_002351"
                     /pseudo
     CDS             1002..1177
                     /locus_tag="ACHKJF_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(1223..1486)
                     /locus_tag="ACHKJF_002352"
     CDS             complement(1223..1486)
                     /locus_tag="ACHKJF_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0949699.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVGTQKGE"
     gene            1676..1915
                     /locus_tag="ACHKJF_002353"
     CDS             1676..1915
                     /locus_tag="ACHKJF_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949700.1"
                     /translation="MNNESEKAMWRCKPVDNPWTFTSLLKKAVCAVLPDFTPAQDKPQ
                     NMFSLSNEKDTDYSVPMRPFALYDPWKHHHDNADF"
     gene            complement(1966..3462)
                     /locus_tag="ACHKJF_002354"
     CDS             complement(1966..3462)
                     /locus_tag="ACHKJF_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP0949701.1"
                     /translation="MTTNNYLEYFLTLLGWVVNNGLWNVLISTGLFALPLAFRIIGIW
                     LKVREEGADEGNKGMLSLPRIEHVLYGAFFVMISCCVPLVQVTLDTLSFDKSRAQTCG
                     VWTPEEPGETGYSGVISSIGDQTAAAPVWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEIQRTFIENRALADELQDFTNDCYSLALYMWKQRDQGQSKDKKVLDDISWIGSATFM
                     QGDYGTLQSKMPRAAFPWNTARDSGRPDTGRGGYPTCREWWSDSSVGLKKRVMEEVSD
                     HYWLRLSAAMKMIGSSQVDYKEEVIKRLVSPANLTLSQGGEVYRGYGGNADMTTMNAV
                     ARIAGTAGSAVAALGAFPALDAMRQALPMVQSIILMAIYIMIPLILAFAAYELSTLIT
                     LTFVVFAVHFLTFWWELARWLDSWMLTALYSSDTHTRFNMMGFQNTSDDLIMNLVMGT
                     MFLVLPAVWLGALSWAGVSVGTVMGDAINKGTSNVSKAAAKSGDLANK"
     gene            complement(3469..3810)
                     /locus_tag="ACHKJF_002355"
     CDS             complement(3469..3810)
                     /locus_tag="ACHKJF_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949702.1"
                     /translation="MSRIYGRVKKRMKVFALYTLLLTGITVFSLWLADTGIRHAEGFA
                     ALREWMYETRYGWLGWRLCLYSAIGWLGWRIWHAPGFRAEYRASFLRMATVSVLFILL
                     CEYAIFAEPGR"
     gene            complement(3815..5236)
                     /locus_tag="ACHKJF_002356"
     CDS             complement(3815..5236)
                     /locus_tag="ACHKJF_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0949703.1"
                     /translation="MKQKMTIPVLAVVLCAGTVQADGNFLSLPKVNDSAFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGVDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGASGAVASLPAMVIQRANPGLYDMLTNGVLQANVAFDKAQLNCQNMA
                     KRMMEFTDSNKWTQAAMLEEYKKIVNTGDGDAIKGDEAGRQVTGEEGQRWIGGQQRGG
                     AGQSAIRPTHDLTAAGFNMMNSLPSTSTSSVSPGNCNGSACMKYRNAEEAARAVVKVL
                     GDKSMRTCGDAKACTSGDADQQPGTSIAGTGFAPMLEEATRVNTELLAGMVNGRDKPT
                     AGNLAKLKAGSLSVTAGVIRALQRDPDNGALTSRLAGELAMADTVETALLMRRMLLTG
                     MAEPYAAAQPAALEEGERRIEALDREIMALKNEMELKRDLARNSVLTIIERDNERVGN
                     NPMIQQADNADSRIHSLDAPDKE"
     gene            complement(5250..6224)
                     /locus_tag="ACHKJF_002357"
     CDS             complement(5250..6224)
                     /locus_tag="ACHKJF_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949704.1"
                     /translation="MISCTSRHRAGVLLLSMAVLPVAEASLNTAQIVASAASQSCISW
                     RVSGICYWLFCTKFSCKVRTSVKVSHYIPEVVVSTYTAPGGNPWREMSAVSSTAGGPE
                     NAISKGIAGLSAGGGNPADMKVQGERRSSIRFKYADATGHPATTLIGGMVPGYSCESP
                     VTPFVPYFLSTLDSIAWRTGLPESLYPEALIPGKREIGSQEEENMWGNVYPRSGFVTQ
                     TDDDKAAAVVAQRVADIITRTGEPHVYQPVKAGKKDGYWPPDPVEENTGTRNHKWQRL
                     TPSVSTSCAVFPDGEHEAAETGNAAFALWQPYSCCKKRGQTFLGSTNF"
     gene            complement(6221..6616)
                     /locus_tag="ACHKJF_002358"
     CDS             complement(6221..6616)
                     /locus_tag="ACHKJF_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949705.1"
                     /translation="MYRLLLYSFLILPVAASAGTTIYTDSHQRPMNPPAGVRVVLLDA
                     PEQTQDTFFGVLPAEPAEASARVMERMQSPEWQSFQAGLAEHYRALAHAWSLGLKKYP
                     AVVFDDSEVVYGTTDVVLAEQLRTGGGQP"
     gene            complement(6752..7384)
                     /locus_tag="ACHKJF_002359"
     CDS             complement(6752..7384)
                     /locus_tag="ACHKJF_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0949706.1"
                     /translation="MLLIAGHLRPAFQAHTGRFLIADLSEEVVRRYLRFCTPLSGMAV
                     SPSSLLLQIRPQMSVLAECLIRQLITDEPSSVYFRDAVAFSSLALFEEHPDFHALLTG
                     CIRSCTVRVKNIISADLSRPWMLGEVASLLYISESLLKKKLQEELTTFSELLLKERMQ
                     YAAQLLSAGENRISRVAELCGYASASYFISVFRRYYGITPNQWLPVMQPD"
     gene            complement(7576..7854)
                     /locus_tag="ACHKJF_002360"
     CDS             complement(7576..7854)
                     /locus_tag="ACHKJF_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949707.1"
                     /translation="MYAEKTILALCISAPFLVAGCDDATKENVSKKAADIKEAAQTQL
                     SDVKDAAEEVKMQAAGKVAEVSEAAQSGLSEVKDTAPEKGAEISVTEE"
     gene            complement(8234..8422)
                     /locus_tag="ACHKJF_002361"
     CDS             complement(8234..8422)
                     /locus_tag="ACHKJF_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0949708.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDEKMTQDECEKLFAPAKEP
                     GRYIGVRVEDFRCEVVKA"
     gene            complement(8800..9177)
                     /locus_tag="ACHKJF_002362"
     CDS             complement(8800..9177)
                     /locus_tag="ACHKJF_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0949709.1"
                     /translation="MLCLKYPEPEVVSAVHPAGSVFVLPPQGAPGISCTTRDNLERLR
                     GHLAAQLGRVECIRCQPQRVGLNSSVAVMLEGQQGQYVHILLTVSGHESWPSEEEYIH
                     PRWYISVTDAADLFYLLLWLGEG"
     gene            complement(9182..11878)
                     /locus_tag="ACHKJF_002363"
     CDS             complement(9182..11878)
                     /locus_tag="ACHKJF_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFP0949710.1"
                     /translation="MTRQDETKVYHACPSVIDFLPWVEYLDEEQCLLLDDGVSVGAVY
                     EVSPAATEGRTAERLEQIRDTVEDALQDSFDEYDSHPWVVQFFCQDENDVDTYVDQLR
                     GYVKPHAEGSSFTEAWLREMERHLKGIARPEGLFRDTLVTGQPWRGQQRRTRMVIYRW
                     IGKNNHDPMPPVAMLNQVCSRVVGALGGAGVRCTRMNGQQVHGWLLRLFNPRPEWVDR
                     DILYRMASRAEPQETPEGMMPVMTDFAESLWFTPPVSDPENGVWWLDGLPHAAVVVEK
                     LRTPPEPGTITGEQARGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENSESLRAREDVATVKKYLGNRHKLYRAGITFLLRAEDMESLKRRRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDTDKKQWYTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCGSLSQIMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGTGVCLPPFADAHQLVEQGETLQSVDEH
                     SLPDLDEDEGDEEEEKRDILGEMEISARMMITGGDPKEEAALKRADRAMIREALLMAT
                     HTTYREGRQMLPVDLQSALWEISRDTQRNDVRRAKAAEMAESLGMFTQPGSFEAELFN
                     REGKLWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDILFVVD
                     EAHIVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDVSEKMLNMAEWWVCLTM
                     PPEEVNNIARFKSLTEEQKAVLLSASKLSGCYTEGVVLAKRTEALFRVVPPSLYLALG
                     MTEKEEKAERRALMKVHQCSELEAAFHVARKLDGLRGLAGRE"
     gene            complement(12016..12408)
                     /locus_tag="ACHKJF_002364"
     CDS             complement(12016..12408)
                     /locus_tag="ACHKJF_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP0949711.1"
                     /translation="MTVMVAVFILPGCSTSKEELLPPGEQSMLEIWQGKGSGGHSRHM
                     TAARDTLRRPLTMPEQQADAHGAISYSRTQESEISQQFPRLPNPDMVMYVFPHLASGT
                     SPVPGYSTVFPFYSRTQYAMPGERTEAL"
     gene            complement(12444..13931)
                     /locus_tag="ACHKJF_002365"
     CDS             complement(12444..13931)
                     /locus_tag="ACHKJF_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949712.1"
                     /translation="MQIKSNTIVKYVVPCVVVAGILTGLKSCGGDTGEPSSAESSGTL
                     AGLSPDELKALGVEGDTPEDTLRTLIGRMNQVRERQLVLDKQNEKLLKENEMLRKRGT
                     DVSGQVSEAVAGLQKNYEQSQRQFRNEQNALMTQVQSLTEQLKRQQESADAGAGGQES
                     DIPAGLGLDGLGSVTGSAASPGTDGLMWVTPADQVQDEASSVGDKSGPRFPVSFLDDN
                     PLTRQKAAYEQQVKGYSNEQQKASTRAVYTLPENSTLAGSRAMTALLGRVPVNGTVTD
                     PYPFKILVGKDNLTANGIELPDVEGAVVSGTASGDWTLSCVRGQVNSITFVFADGTVR
                     TLPSSDVNGSSNAQAKNNSSTGGIGWISDDDGIPCISGTRKSNASTYLPTIFALSAAG
                     AAGDALSEGQYTSQNNVNGITSTLTGSAGEAALGKALSGGMNETAEWVKQRYGQTFDA
                     IYVPPGQTLAVHITRQLAIDYEDQGRRVRYDFTLPGEVHGTGGLD"
     gene            complement(13921..14829)
                     /locus_tag="ACHKJF_002366"
     CDS             complement(13921..14829)
                     /locus_tag="ACHKJF_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949713.1"
                     /translation="MNGAERCLYRSIVMVLSLLVLPLSARADELMKWERIPLPVTLMA
                     GQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKAESVFPQTRLQLQDVESGEIIL
                     LDVTAAEKGPTEPVRIVYEGSVSTVSQAGTAASARSAEHKKVVPGGDDGSQALRQPVK
                     YAEPLPVLLTRYAAQSLYAPLRTLEAVPGIRPVNTHLPGKITTLYPSEPLTVTPLAAW
                     AAGGRAVVALKLTSTVSRKIILDPRALQGKFIAATFQHRWLGAAGTPEDTTTLYVVTA
                     ERPEQAFIAEPASRRIPSRGEVKHAD"
     gene            complement(14829..15491)
                     /locus_tag="ACHKJF_002367"
     CDS             complement(14829..15491)
                     /locus_tag="ACHKJF_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949714.1"
                     /translation="MSRFRHALRDRDQHILTLRIFSGVLAGILMLVCIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILESDARRRSDAGELLERVRVVYEIPEHGYGGRYNKNPVTVEGRDNWQVQLDLV
                     ADEYFHAEPVKRALVRYPIKVVRMEGDPERNPFGLALDCYSSTPQRLEAASPAAGKGD
                     NQ"
     gene            complement(15488..15850)
                     /locus_tag="ACHKJF_002368"
     CDS             complement(15488..15850)
                     /locus_tag="ACHKJF_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP0949715.1"
                     /translation="MQTIRFLPDRLNVEPAVFRGFTTPELGLAALTGAVAGGIWPLPL
                     IPLLGWVIIPTGMLLTPLLLVWFGGVWISRLKRGKPDNWLWQHLAKKSSAWGGGRKTF
                     IREAHGWSLRRTRSRGKK"
     gene            complement(15863..16177)
                     /locus_tag="ACHKJF_002369"
     CDS             complement(15863..16177)
                     /locus_tag="ACHKJF_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0949716.1"
                     /translation="MAVFLCTGITPALADLPQIEPPTSGGGGGLFGQIKGYLQDGVVL
                     FGLVLSAVAFVKVAEAALSTFSEVRDGKAGWGQFGAIVVVGVVLLVAIIWLLGKSASI
                     IL"
     gene            complement(16254..16496)
                     /locus_tag="ACHKJF_002370"
     CDS             complement(16254..16496)
                     /locus_tag="ACHKJF_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949717.1"
                     /translation="MAMTALQNTAFRAASGEVDLSVLHLITLGFLLAVVFLWAAWSLT
                     DAWAGWRSGDVRDGVFGWFMFRLVLLLICSIWMFAG"
     gene            complement(16496..16837)
                     /locus_tag="ACHKJF_002371"
     CDS             complement(16496..16837)
                     /locus_tag="ACHKJF_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001720563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFP0949718.1"
                     /translation="MFSFPASPCRAGRLAGAAGMLFLLSFALPAQAAEKDELATVMRQ
                     LDHVQAALERARTASLQASSQGERYFFDYLRARADLQTIRDGIDHYLAPSRAQPRSSA
                     AVTGDYRRERP"
     gene            16873..17124
                     /locus_tag="ACHKJF_002372"
     CDS             16873..17124
                     /locus_tag="ACHKJF_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949719.1"
                     /translation="MRKAEDWISDKLYLQNRKFLRENTLVWMKANNPGNTGNTGNTGN
                     TGNTGNTGNNKLCSGGCQHSQFLHLAVPDSVQINLFQPE"
     gene            complement(17488..18249)
                     /locus_tag="ACHKJF_002373"
     CDS             complement(17488..18249)
                     /locus_tag="ACHKJF_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0949720.1"
                     /translation="MAEPRREVNRAAQPSPPRQHGLLWNLLWGWPWMLVGILFSSLLL
                     SLLVEYIGIAFFWPEQGAAHSEAVMKTELGWLSSEFTRSLLLSTPSVTVTEWIATAYQ
                     WLFVETGFLSWLKQQNAGAVVGNSVSQYSEDVGRWLSHYLCEYLMATVYVTVVTLIRV
                     TILVLSVPLFVMVILIAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCIFY
                     LSWPTSVYPNLLIWPAAMLLGVAITVTMASFKKYL"
     gene            complement(18242..20338)
                     /gene="traD"
                     /locus_tag="ACHKJF_002374"
     CDS             complement(18242..20338)
                     /gene="traD"
                     /locus_tag="ACHKJF_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001510361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0949721.1"
                     /translation="MSNRYVIEALLRPAVELNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAVVRFRQGMKIIRYRRNLRRLPRYVMSSRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGSLYRTARWLEMKTEHSLPWIGQLLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGDVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIVARALVALGERPDYTLIMRYVNNIADLYIRYAGKVI
                     SEQMPELENAILNNMNVLGEADVPRTMQNQPDAVRIWAIEVALSSEAGKKLYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGKTAELLAPDYLDMSDTRPVFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMNAGLPGGNGTKNPVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARTGSAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLSQVEIYTKTLVSGHQDTADVTTGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPATDADDALMPESIAKIAEAMRRNYHTGEGW
                     WNETPSVNMPEGEQNG"
     gene            complement(20335..21123)
                     /locus_tag="ACHKJF_002375"
     CDS             complement(20335..21123)
                     /locus_tag="ACHKJF_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949722.1"
                     /translation="MKGAFTERIQANAPAWAHEQLVAWDTAEFAAMSDFITEHYWTGQ
                     GSINVYRIVGTDHPQYAGMSWLELLERGKRMDINIPLLEKNPGYYTQAEQQHAGMGFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEGRTQLHNVAQSIYHTDQAFRSACREIHNL
                     AEPLSRHDVYLRLQIRRQCVSREDLACWKVDRFRTEALLTVDDVHAGGHDGPLVYKAL
                     LLNAADAWREVMVLQRRLEALSASPEKALPRSWWRRLLQRGTRS"
     gene            complement(21120..21602)
                     /locus_tag="ACHKJF_002376"
     CDS             complement(21120..21602)
                     /locus_tag="ACHKJF_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949723.1"
                     /translation="MIMTLYRFVMLLLRLLIVWPSMLAGCLVMLYVWNNLSGGEAGMT
                     QRYATESSRWRDAPEGHVMVQKCSSVISVSGMYVDEQECPRTAISFGEAVAEDIRELK
                     MQAVVLLLGALFMEGVSSVILFRGCGSAAVSGEGRGGPAVMPWPDGIGISKVQERRAE
                     "
     gene            complement(21599..22192)
                     /locus_tag="ACHKJF_002377"
     CDS             complement(21599..22192)
                     /locus_tag="ACHKJF_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0949724.1"
                     /translation="MMPLSATSAALSASPVLTACLVLSLLAVVLGPALWRSRRKKACV
                     RTRRHQRYRATASRVMKRLNELQGDARRLKYLRKISPYVFEELLLLAFEQQGMPVIRN
                     PSYSGDGGLDGQVVINGERWLIQAKRYGRTIRPAHIDAFGELLRRKGCRGFFIHTGRT
                     GNISRARLTLWPDIELISGQRLLQLLNGTLLSNKGRS"
     gene            complement(22189..22707)
                     /locus_tag="ACHKJF_002378"
     CDS             complement(22189..22707)
                     /locus_tag="ACHKJF_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0949725.1"
                     /translation="MKRERQFYVLSLLLFCTTAGAELKVIADLGGRDASPFFEAINRQ
                     PPVPASVVPSPSFPQGEAAMLPVSTPEMSPGEVVARRLQLPGIGALFLVGDDAHSRTW
                     LQMNAAVLRERKAAGMVVNVADESSLRVLRELAPGVVMVPASGSVLARRLQISHYPVL
                     ITDNGLSQVPAP"
     gene            complement(22704..23384)
                     /locus_tag="ACHKJF_002379"
     CDS             complement(22704..23384)
                     /locus_tag="ACHKJF_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0949726.1"
                     /translation="MARGGILTVLLMVACSGVCRAAVQNVPDGYVRVARAHGIPAELL
                     YAVSLTETSMSPEAMSGVLKQRRRDFRQPDVARPWPWTINVAGKGYRYATRLEAWQAL
                     QVFLKHYPAKRIDVGIAQVNLGWNGHRFPSTWAAFDPYTSLNTAAVILQECRRRHPVS
                     WLQVAGCYHHPAGGAPAARYTAVVRRHLARLHRDNFLNPSDVHVAATAMLTPSPRYIW
                     TEPQEEQK"
     gene            complement(23363..24082)
                     /locus_tag="ACHKJF_002380"
     CDS             complement(23363..24082)
                     /locus_tag="ACHKJF_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016191739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949727.1"
                     /translation="MKRKILYLLALCPVLAQSAGQTNMSSEAQRTTGTVVTQSTTSGL
                     QQQAGQWGVSEEDYRRYQSLMSGPRGIQSPGLDPLTVLGIEARTPAERRVWAEKWVRA
                     EYERTEKELAFQQEVNAAWKRLYPSILPVSMGKTGALTGDTGGRLALFVKAKECGTCD
                     ARLASVLATGRQVDIYLVDSQGKDEILRQWAHAHSIPVEKVRSRHITLNHDAGRWLRF
                     GEGKMPVVLQQGADGWRVAAF"
     gene            complement(24086..24835)
                     /locus_tag="ACHKJF_002381"
     CDS             complement(24086..24835)
                     /locus_tag="ACHKJF_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949728.1"
                     /translation="MSEKQPQIPEGASGMSPERRKSRFPGVLILSVTVGLVMGAVVYG
                     VLMLMTGLDSRITTLEGQRREMLSASELAPLRMKLTETEKQLKDMEKRLAVLEQQPVL
                     TASWKEQLENLPEMLEQIESAREKLSLRLDTLSGLVEKMQKVPESPSQPDSRKAGMKK
                     TEEKKPAPVTSSRKSRAVHTVRQAPFVLTGTELRGGSTRAAVAPRGYTSLSQVALLGE
                     GESVAGWTLVYAGHGEATFRVNGRQTVLRVE"
     gene            complement(24832..25431)
                     /locus_tag="ACHKJF_002382"
     CDS             complement(24832..25431)
                     /locus_tag="ACHKJF_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFP0949729.1"
                     /translation="MKKRFILSGLTVCLLMAGCQSTQKLQQRDAAMPVPSVTVNRNVQ
                     PASPDVYSQAPEVVRYDRYLLVSTEPQAVQRDPLSQVIDIRIPSSLKPTVADAMRYAL
                     KQSGYTLCATGPANGVLYRQPLPAVQYQPGPLRLRTALQLMAGPAWQLEVDDVQRVVC
                     HSLRDGYQLPVSQLAPVPVTVPFVGREPGVKATGGFLKK"
     gene            complement(25617..26585)
                     /locus_tag="ACHKJF_002383"
     CDS             complement(25617..26585)
                     /locus_tag="ACHKJF_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0949730.1"
                     /translation="MNENTPTTPTPHDAAFRAMMECPSVARDFLKGALPPEQLNRCDM
                     NTLQLEPTTFVDPDLRQFACDVLWSMKTTDGRDGYIYTLTEHQSKADKYMALRMMRYV
                     LAVIHRHLKAHACAPVVIPVLFYHGVPSPYPYSMNWPDCLDDPAFGRELYGEGRPPRV
                     IDVGLLDDEGIRRYKQMAALMLMMKLRQKKVDLMVHLDFLSQLIQIQETHTQVVTLFN
                     YMVKECDSASPEFIRAMAERLPRHEDDLMTIAERLELAGIEKGRQEGIEKGRLEGIEK
                     GRQEEREKALQENYASARRMQQMGISSEKIKQILQFSDDDLRNALR"
     gene            complement(26634..27194)
                     /locus_tag="ACHKJF_002384"
     CDS             complement(26634..27194)
                     /locus_tag="ACHKJF_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0949731.1"
                     /translation="MASRGVNKVILIGHLGQDPEIRYFPNGGGAVANLTLATTDTWRD
                     KQSGEMKEHTEWHRVVIYGKLAEIAGEYLRKGSQVYIEGQLRTRKWFDQQNGVDRYST
                     EVVVSVNGTLQMLGSPRQQQGGGNVSSAPAQGGWGQPPQNAQQSTGKNGQASSGSGGM
                     PPMDFDDDIPFMGRGYGVERRIIHVI"
     gene            complement(27210..27413)
                     /locus_tag="ACHKJF_002385"
     CDS             complement(27210..27413)
                     /locus_tag="ACHKJF_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949732.1"
                     /translation="MKPTSGILAAKAGYTLPLQILSSARGYYIGTQCDEGPVSRESEE
                     YFRNYEQAVRALENGIWTQRECV"
     gene            complement(27504..27971)
                     /locus_tag="ACHKJF_002386"
     CDS             complement(27504..27971)
                     /locus_tag="ACHKJF_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFP0949733.1"
                     /translation="MTANTSSANAKTEYFNLTLKGLGYLSNIRQVNHQNGTFLSCVVN
                     ALSGPTDSPSYVRFDVTVAGKEATSLIARCQKAVDEDRKVLIGFTMSNPSTDIFTLNR
                     GEHAGEQRVSLKARLIKVEWIKIGQDMVYKTEKSESIPPQNGNTPREYAENSF"
     gene            complement(28650..30692)
                     /locus_tag="ACHKJF_002387"
     CDS             complement(28650..30692)
                     /locus_tag="ACHKJF_002387"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0949734.1"
                     /translation="MQLFLCEKPSQAKDIARELALSKRGDGYIGDDNIAVTWAIGHLL
                     ELAEPEAYGEQFGSPWRSDVLPLIPEQWQMVVKPKVKKQLIVIRGLLKRASEVVIATD
                     ADREGEVIARELLEYCQYRGRVRRLWLSALDEASIRKALNNILPGEQTALLSEAGKGR
                     ARADWLTGMNLSRLYTLKARERGISGVLSVGRVQTPTLAMVVRRDQEIATFVPVSWWQ
                     VIVWLEKEGLRFRSNWVAAEQYCDDEGRCVNVQAAGAVLGLCRQQSSAVVQDVVQKRE
                     KTPPPLCFSLGTLQEACSRKFGMGAQTVLDIAQSLYETHKATTYPRTDCGYLPASMHE
                     ESSEVLEAVGRSDPSVLPQLAELESGRVSRIWNDKKITAHHAIIPTRQVFDLSSLNGD
                     ELKVYQLIRQHYLAQFLPEQEADVTEATFVIGGQLFRSRGRVNVIIGWKQLFPPENHA
                     DEEKPDSDESGNILPPLIKGELCALDGAEQKELKTVPPRPFTEGCLITAMKNASSGVT
                     DPALKKILRENAGLGTEATRAGILETLFARNYIERAGKHIRSTQLGRELVAALPDSLT
                     NPGMTALWEQALDDVARGKISLEAFMEKQHQWIRHLVLSGRNQTVNLSVESGPSCPLC
                     NGPTVKRKGKSGFFWGCSRYPDCRGIAGEQGNSRGRKRKINFSHKQKSGDVGDKLN"
     gene            complement(30695..31399)
                     /locus_tag="ACHKJF_002388"
     CDS             complement(30695..31399)
                     /locus_tag="ACHKJF_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFP0949735.1"
                     /translation="MPQEKTGEVRKSEQVGALQSSLTIALHTRYAIRLWEGRRNNQKE
                     EKQEKRPDIISMPRAIAFAGQATRDSARDNPYADMMLVRLENALTKATETIEKHLAWL
                     DGILKNLPGQISLSDIKSSSPVNIGVYSSSPVGYRCVWLLIGYDRLVMKVFQVFHYGL
                     ISRTKRDELLNQGGYAVRRIYGIIQNYKSVSVTRADILSGTARGKEAVNRMGEPDPDV
                     MSGKKRSVYSPPVKAG"
BASE COUNT         7387 a         9793 c         7233 g         7321 t
ORIGIN      
        1 ggcgtataaa ttcatcattt tcaaaatatg ttaattttat cagctcttta tttatacttt
       61 taactggcgc atatctggtt tttttatgat atgccgtcag ggtgtgcttc cttacatatc
      121 acgccctctt ccagacgtcg ctggtcgtcg ccctctgcgt cccttacttt cagcccctct
      181 gagcataatc gcccctccag aggagcccat cacaacgaca atgccagcgg gttaatttta
      241 tctgacccgg ccttcagcca catccttcac acgtttaact aatggctcaa taaatttatc
      301 tttcagatgc cccggtgccg gagatgcata cgttatatat atccgacttc ctttgccatt
      361 tttttcaagt tcaagcgtca gatgatcatc aaactcgcca ccatcccagt cactgcccat
      421 tttataataa ctaccgggca tggcatccca catatgcccg ttctgactta ttgctgctgc
      481 ggcccacttc ccgttcgcat gtccgttatt acgaatcgca tcgacttctt catttgaata
      541 aaacttatag taccgtttca gtcttcccgc agctgtatcc acatcaacag ggacttctat
      601 gacatgatca cgtttctctc tttttgcccg gggtgcctga gacagatatt tgagatcatc
      661 atctgcctgg tttacaccta cgttcagtac ttttgtcaca gcaaatgcac cgtcacttat
      721 ctgcctgttc agctctgcaa tttcctgtgc agaacaggca ctcagcgcaa cacacgaagc
      781 acaaacgagt aacatttttt tagtcagcat gtcttcatcc tcatcagcag ctcttcagtg
      841 gataacatcc acagttacac ctgcatttac gcgcaacaaa aacaccagat gaagatttta
      901 ttcattcagc tccgctaaaa attaatcttg ctgtggaata tcgggaaaac aggaagttac
      961 gttaaaagcc gaaggaaaaa ctcacaatcc cttcttcaat ctacatcagt atcatgccca
     1021 aaccagacgg gttaacggca gcaaagaacc ttgcagaagc gttcgagcat tataacgaat
     1081 ggcatccaca tagtgcgctg ggttatcgct ctccacggga atatctgcgg cggcgggcag
     1141 taatgggtta agtgatacca gatgtctgga aatataggag caaatccacc acgcatggag
     1201 cctgttaaga ctctcactct catcattctc ccttctgagt cccaacgaaa cgaccaaaac
     1261 gctgagtaaa gcgggcaaca ttgccttctg atgccaccgt tctgagttta ccggtataga
     1321 acggatgcga tttggaggaa acgtcaatcg tcacatacgg atacgtcaca ccatccagtt
     1381 caatctcgcg atctgttttg atggtagaac ctattttaaa gtactcatcc acactcgtgt
     1441 catggaacac tactgtgcgg tattcaggat ggatatttgg cttcatctct cttcactcat
     1501 gttataacat aacaataaga tacattctca gctagatcat ttcaacagaa tccccgagcc
     1561 caagggtaat gtggcatcgc agcctctcat caaatggaag acatgtgatt gcacatttat
     1621 catacaagac gtatgataac actgtgttat ttatcctgct gctgagggta tagatatgaa
     1681 taacgaatct gaaaaagcaa tgtggcgttg taagcctgtg gataatccat ggaccttcac
     1741 ttccctgctg aaaaaagcag tctgtgccgt tttaccagat ttcacacctg ctcaagataa
     1801 accccaaaat atgttttcac taagtaatga aaaagatact gattattcag taccaatgag
     1861 accctttgca ttatatgacc cgtggaaaca tcaccatgac aatgctgatt tctaagaaaa
     1921 gcaagggttt ccccaattat cccagctaag gaaaccctaa gaattttact tattagctaa
     1981 atctccactt tttgctgctg ccttagatac attacttgtt cctttgttaa ttgcatcccc
     2041 cataaccgtt ccgacactta ccccagccca ggacaacgct cccagccaca ctgccggcaa
     2101 gacgagaaac atcgtcccca tcaccagatt cattatcaga tcatccgatg tgttctggaa
     2161 tcccatcata ttgaaccggg tatgtgtatc cgaactgtac agcgccgtca gcatccagct
     2221 gtcaagccac cgtgcaagct cccaccagaa tgtcagaaag tgtaccgcaa acaccacaaa
     2281 cgtcagtgtg attaacgtcg acagttcata tgccgcaaac gccagaatca gcggtatcat
     2341 gatgtaaatc gccatcagta ttatcgactg taccatcggc agtgcctgcc gcattgcatc
     2401 cagtgccgga aacgcaccca gtgctgctac cgcactgccc gccgttcccg caatccgggc
     2461 caccgcattc atggtcgtca tatctgcatt gccaccatag ccacgataca cctctccccc
     2521 ctgagagagc gtcaggtttg ccggactcac cagacgcttg atcacctcct ccttataatc
     2581 cacctgacta ctgccaatca tcttcattgc agctgacaga cgtaaccagt aatgatcact
     2641 gacctcctcc atgacccgtt ttttcagccc cacactactg tcactccacc actcccggca
     2701 tgtcggatag cctccccgcc ccgtatccgg tcgtccgctg tcacgtgctg tattccacgg
     2761 gaacgcagcc cgcggcatct ttgactgcag cgtcccgtaa tccccctgca tgaatgtggc
     2821 gctccctatc caggagatat catcaagcac ttttttatct ttactctgcc cctgatcacg
     2881 ctgtttccac atatacagtg ccagtgaata acagtcgttc gtaaagtcct gcagctcatc
     2941 agcaagtgcc ctgttttcta taaacgttcg ctggatctca aaacgcaact ggcgcaaatc
     3001 aggccggcac ggaatcgaag ccactgccgc ctgggtgaca cctttcgaca atttatgaac
     3061 caccacccac cataccgggg cagcagcagt ctgatcccct atactgctga tcaccccact
     3121 gtaccccgtc tctccgggct cttcaggtgt ccatacccca caggtctgtg ccctggattt
     3181 atcgaatgac agcgtatcca gcgtcacctg taccagagga acacagcagg atatcatcac
     3241 aaaaaaagca ccatagagaa catgctcaat acgcggcagg gaaagcatcc ctttgtttcc
     3301 ctcatccgca ccttcttccc tgactttcag ccagatcccg atgatcctga atgccagtgg
     3361 cagggcaaac agtcctgtgc tgatcaacac attccacaga ccgttattca ccacccagcc
     3421 cagcagagtc agaaaatatt ccagatagtt atttgtggtc atgattgctc aacgccccgg
     3481 ctcagcaaaa atggcgtatt cacacagcag gataaacaga acactgactg tggccatacg
     3541 cagaaaagaa gcccggtatt ctgccctgaa ccccggcgca tgccagatcc tccaccccag
     3601 ccatccgatc gcactgtaaa gacacagacg ccatcccagc cagccataac gtgtctcata
     3661 catccactcc cgcagggccg caaatccttc tgcgtgacgt ataccggtat cggccagcca
     3721 gagggaaaac acggtaatcc ctgtcagcaa cagcgtatac agggcgaata ccttcatcct
     3781 ctttttcacc cggccataaa ttctgctcat cctctcactc cttatccggg gcatcgagag
     3841 aatggatacg gctgtcagca ttgtcagcct gctggatcat cgggttattc cccacacgct
     3901 cgttatcccg ttcaatgatg gtcagaacac tgttacgcgc cagatcccgc ttcagctcca
     3961 tctcgttttt cagcgccata atttcccgat ccagcgcctc aatacggcgt tccccctctt
     4021 ccagtgcagc aggctgagct gcagcatacg gctcggccat ccccgtgagc agcatcctgc
     4081 gcatcagcaa cgccgtttca acggtatccg ccatggccag ttctcccgcc agacgcgacg
     4141 tcagggcacc attgtccgga tcacgctgca gggcacggat cactcccgct gtcacagaca
     4201 gactcccggc tttcagtttt gccagattcc cggctgtcgg cttgtcacga ccattgacca
     4261 tacctgccag cagttccgtg ttaacccgcg tcgcttcttc aagcatcggc gcaaaccccg
     4321 tgccggcaat actggtcccc ggctgctgat cagcatcccc gctggtacat gcctttgcat
     4381 caccacaggt ccgcatggat ttatccccca gaaccttcac caccgcccgg gcagcttcct
     4441 ctgcattacg gtatttcatg caggcactcc cgttacagtt cccgggactg acactggatg
     4501 tactggtcga aggcagactg ttcatcatat tgaaaccggc agctgtcaga tcatgtgtcg
     4561 gccggatagc agactgtccc gctccaccac gctgctgccc tccgatccag cgttgcccct
     4621 cctccccggt aacctgacga cctgcttcat cccctttgat cgcatcaccg tcccccgtat
     4681 tcacaatttt tttgtactcc tcgagcatgg ccgcctgcgt ccacttattg ctgtccgtga
     4741 actccatcat gcgtttcgcc atgttctgac aattgagctg cgctttgtcg aatgccacat
     4801 tcgcctgcag tacaccgttg gtcagcatgt catacagccc cggattagcc cgctgtatca
     4861 ccattgccgg cagactcgcc acggctccgc tcgccccctg aatcacatcc cccatcaggt
     4921 ttttgaaacc atcagtgatc ccgttcagct ggttacccac tgttgttttc aggtcaaaat
     4981 tgccacacat cagatcactg ctccacccga gatccacccc aagtttctgc atattgctgc
     5041 gcgttgccgg ctgcgatatc accgagcccc ctcccagggt ataaaacagt ttgtctgata
     5101 ctgcgccgtc cacggattta ccgtagccaa acgcactgtc attcactttc ggcagggaca
     5161 aaaagtttcc gtcagcctgt accgtacccg cacaaagcac cacagccaga acaggtatcg
     5221 tcattttctg tttcatcact tccccttact taaaaattcg tactgcccag gaatgtctgt
     5281 ccccgtttct tgcagcaact gtagggctgc cacagggcaa aggcagcatt acctgtttca
     5341 gccgcttcat gctctccgtc agggaagact gcgcaggacg tggagactga aggcgtcagt
     5401 ctctgccatt tatggttccg ggtgcccgta ttttcctcca ccggatccgg tggccagtaa
     5461 ccgtccttct tccctgcttt caccggctga tagacatgtg gttcaccggt gcgggtgata
     5521 atgtcagcca cccgctgggc caccaccgca gcagctttat catcatctgt ctgggtcacg
     5581 aacccggagc ggggatacac attgccccac atattctctt cttcctggct gcctatttcg
     5641 cgtttaccgg ggatcagggc ctccgggtac agtgactccg gtagtcctgt acgccaggca
     5701 atactgtcaa gcgtactgag aaaataagga acaaaaggtg tcaccggtga ctcacaggaa
     5761 tatcccggca ccatccctcc gataagcgtg gtggccgggt gccctgttgc atcggcatat
     5821 ttaaaacgga ttgacgaacg acgttccccc tgtaccttca tgtctgcggg gttccctcct
     5881 ccagctgaca gaccggcaat acctttgctt atggcattct ccggaccacc ggctgtactg
     5941 ctgaccgccg acatttcacg ccagggattt ccgccaggtg cggtataggt ggaaacaacc
     6001 acttccggga tataatggga aactttcact gatgttctga ccttgcaact gaatttggtg
     6061 cagaacagcc agtagcaaat cccgctgacc cgccagctga tacagctctg tgaggctgca
     6121 ctggccacta tctgcgcggt gttcagcgat gcctctgcca ccggcaatac cgccatactt
     6181 aacagcagaa caccggcgcg atgacgtgat gtgcaggaaa tcatggctgc cctccccctg
     6241 tacgcagttg ttctgccagc accacgtctg tggttccgta gaccacttca ctgtcatcaa
     6301 aaaccacagc cggatatttt ttcagtccaa gggaccaggc atgtgctaat gcacggtagt
     6361 gttcagcaag ccctgcctga aacgactgcc attcaggcga ctgcatccgt tccatgacac
     6421 gggcagatgc ttctgcaggc tctgcgggaa gtaccccgaa aaatgtatcc tgcgtctgct
     6481 caggagcatc aagcaggaca acacgaacac ctgctggcgg gttcatgggg cgctggtgcg
     6541 aatcggtata aattgttgta ccggcagatg cagccaccgg tagtatcagg aaggaataca
     6601 gtaacagtcg gtacatgatg cgactccgtc agacaactgg tatctgacag cgtgcgcaaa
     6661 ggcaaaggga caatcagcaa taaattcaaa accggatgat aaaaattaag cctcttcctc
     6721 tgaataacgc acactctgca tctgaatcaa attaatccgg ttgcatcaca ggaagccact
     6781 gattcggggt aataccataa taacgcctga acacagaaat aaaataagaa gcactggcat
     6841 agccacacaa ctctgccacc cgggaaatac ggttttctcc cgcagacaaa agctgagcgg
     6901 catactgcat tctctctttc agaagaagct cactgaaggt cgtcagctct tcctgaagct
     6961 ttttcttcag taaactctca cttatatata acagactggc cacttcaccc agcatccatg
     7021 gacgggacaa atcagcactg ataatattct tcacccggac agtgcagctc cggatacacc
     7081 ccgtgagcag ggcgtggaaa tccggatgtt cctcaaacag cgccagactg gaaaaagcca
     7141 ccgcatccct gaagtacacc gacgatggtt catctgtaat aagttgtctg atcagacatt
     7201 ccgccagcac ggacatctgt ggccggatct gtaaaagcaa agacgacggg ctgactgcca
     7261 taccggataa tggcgtgcag aaccgcagat accgcctcac cacctcctca ctgaggtcag
     7321 cgataaggaa acgtccggta tgagcctgaa aagcagggcg cagatgcccg gcaatcagaa
     7381 gcattgtgcc ggcaggtatg acagagttcc tgtgctccag acaaagcatt gtctgtttga
     7441 gagccataat gacaacgcag gtacagggca tcggatatcc ggtaaatatg ttgatttatt
     7501 aaagagacag acatgccccg tatataaaat tccgtgtcac cgggtgctga aaaaaactgc
     7561 accgacggcg ctcttttact cctccgttac ggatatttca gccccctttt caggagcggt
     7621 atccttcacc tcgctgagcc cggactgagc cgcttcagaa acctctgcca cctttccggc
     7681 agcctgcatt ttcacttcct ccgcggcatc cttcacatca ctgagttgag tctgcgcagc
     7741 ttcctttata tctgccgcct tcttacttac attctccttt gtcgcatcat cacagcccgc
     7801 aaccagaaat ggtgcggaaa tacataacgc cagaattgtt ttttcagcat acaatctctc
     7861 attattaagt gttaacgcag aacagcttat atttcgtccc cgggaaatgg tcaaaagaca
     7921 accagaacat cacagtgtca gggcacactc ccggtatatg cttttataac cataacatgc
     7981 tgttcctgat attaccggaa atgtgcccac tacatttaac ctgttttcag caccgggtca
     8041 tcggctgtgc tgtatttttt attcccgtca cagaaacaca ctattaccgt tgatttcatg
     8101 acgaataaaa tgaataaatt ctcccgtggt ctttaccacc attattatac caataacacc
     8161 atttacacag atacactccg ggactatctg tttttgcccc ctccgactcc gccggaggtt
     8221 tttattttca tactcaggcc ttcaccactt cacaccggaa gtcctccacc ctgacgccaa
     8281 tataccgtcc cggctctttt gcgggcgcaa acagtttttc acattcatcc tgagtcattt
     8341 tttcatcctg gtaacgcagc cactgaaccg gcatattgcc tggtttgatt attgtcgcag
     8401 ttattttata tcgctttccc atactggctc tcatctcaac aacaaaatca ccggctgagc
     8461 catcactccg gtaatgcatg cgaaaaaaag tgagcgtctc agcaatggct catgcggtga
     8521 ttctgtacgg tacccgcaca aaatacgggc accaacagcg tcgcagaata caacgaaggc
     8581 atcagggcag cacctctctc ccgctatttt tcagagttct ttaccctcct cctgacacgg
     8641 gagtacaacc ctgacagcat ccgggccagt acattcgcaa gcccccatga aaacacagcc
     8701 acacccacag ctcccgccag aaacagcaca ggttcactgg caatttgatc tgacagcgat
     8761 ggatacagtg gtcccggaaa atcgatatcg ctcctcatat cacccctctc ccagccacaa
     8821 cagcagatag aacaaatccg ccgcatcagt cactgatata taccagcggg gatgaatata
     8881 ctcctcctcc gacggccagc tctcatgccc actcacagta agcagaatat gcacatactg
     8941 accctgctga ccttccagca tcaccgccac actgctgttc agacccaccc tctgtggctg
     9001 gcaacggata cactccaccc gccccagttg cgccgccaga tgcccgcgaa gacgttcaag
     9061 attatcccgt gtggtgcaac ttatccccgg cgcaccctgt ggcggcagaa caaacacact
     9121 tccggcgggg tgaacagcag acaccacttc cggttccgga tacttcagac acagcatagt
     9181 ctcactccct gccagccaga ccacgtagcc cgtccagctt tcgtgccaca tgaaacgctg
     9241 cttccagttc gctgcactgg tgcaccttca tcagggcgcg gcgttcagcc ttctcttctt
     9301 tctccgtcat ccccagagcc agatacagac tcggtggcac aacccggaac aacgcttctg
     9361 tgcgcttcgc cagtaccacc ccctccgtat aacagccgga cagtttactg gctgacagca
     9421 gtaccgcctt ctgctcctcc gtcagggatt taaaacgggc aatattgttc acttcctccg
     9481 gcggcatggt caggcacacc caccactctg ccatattcag catcttctcc gagacatccg
     9541 gataatcctt caggttctgc gtggccagcc acagccaggc ccccagcttg cgccacattt
     9601 tcaccacttt ggtcatatac ggggacaaaa gcgggtttac cgtcacgata tgcgcctcat
     9661 ccaccacaaa cagaatatcc cgtcccagat actgatcgcg ctcggcaatg ttgttaatca
     9721 tattagtcat tgacaccatg gtcagtgcca tctgggcttc ataaccttca cgggcaagat
     9781 gtcccagatc aatcagggtc acatccgctt ccggccacag ttttccttcc cggttaaaca
     9841 gctccgcttc aaaactgccc ggctgggtga acatccccag cgactctgcc atctcagcag
     9901 ctttagcccg gcgaacatca ttacgctgag tgtcccgcga aatttcccac aacgcactct
     9961 gcagatccac cggcagcatc tgacgcccct cccggtacgt ggtgtgcgtg gccatcagca
    10021 gtgcctcacg gatcatcgcc ctgtccgcac gtttgagcgc ggcttcttct ttcggatccc
    10081 ctccggtgat catcatgcgg gcagagattt ccatctcccc cagaatgtcg cgtttctctt
    10141 cctcttcatc cccctcatct tcatcaagat cgggcagtga atgctcatcc acactctgca
    10201 gcgtctctcc ctgctccacc agttgatgag catccgcaaa tggaggaaga cacacccccg
    10261 ttcccggttt gacactgatt ttattaaccg aaagcccgag actctcaaaa taatccgcca
    10321 gcagaccaaa cgagttcccg gcctcagcga taaacagacg gggacgatga actgccatta
    10381 tctgcgacaa cgaaccacac agcgtggccg atttaccggc tccggtaggc ccgaaaagca
    10441 gcagatgtgc attctgggtc cggtccagct tgttcagcgg atcaaaggtc agcacatccc
    10501 cgccacggtt aaagaaactg aatccgggat gccccgtacc ggtttccctt cccgtcaccg
    10561 gcaggagccc tgccagatgc tgtacccaca tcagacgcgt gtaccactgt tttttatccg
    10621 tatcaggatc aaaacacatg ggcagcgcgc gcagccagct gttcagcggt ccgacatcaa
    10681 actccgggcg cactggctgt aagcccgcgc ccagcagcac cgtcgtcagt tcaacccgtc
    10741 tgcgcttcag actctccata tcctctgccc gcagcagaaa cgtgatcccg gcacggtaga
    10801 gcttgtgccg gtttcccaga tatttcttca cggtcgccac atcctcccgg gcacgcaggg
    10861 attcactgtt ctccccgacc gcatttttcg acaacctcgt gaagcgctct tccagtgtgt
    10921 cctgtggctg gaccacaatt gtcatacaca gcaccgttcc ctccggaaag gtgtccatca
    10981 gggcattcac tgtcttttcg ccccgggcct gttcccctgt gatggttccc ggctccggtg
    11041 gtgtgcgcag tttctccacc acaacagcgg catgcggaag cccgtccagc caccacacgc
    11101 cgttttccgg atcagatacc ggtggggtga accagaggct ttctgcaaaa tcggtcatca
    11161 ccggcatcat tccctccggt gtctcctgcg gttctgcacg ggatgccata cggtacagaa
    11221 tatccctgtc cacccattcc ggccggggat tgaacagcct cagcagccag ccatgcacct
    11281 gctgcccgtt catccgtgta cagcggacgc ctgcgccacc cagcgccccc acgacccgcg
    11341 aacagacctg gttcagcatt gccaccggcg gcatcggatc gtggttgttt ttcccgatcc
    11401 accggtaaat caccatcctc gtgcgccgct gctgaccacg ccatggctga cctgtgacca
    11461 gcgtatccct gaacagacct tccggtcggg caatcccctt cagatggcgc tccatttccc
    11521 tcagccaggc ctccgtaaat gaggagcctt ctgcatgagg tttcacgtac cccctgagct
    11581 ggtcaacata cgtgtccacg tcgttttcat cctgacagaa gaactgcacc acccagggat
    11641 gtgagtcata ttcatcaaaa ctgtcctgca gcgcatcctc caccgtatcc cggatttgct
    11701 ccagacgctc tgcagtccgc ccttccgtag cggccggact cacctcatac acggcaccaa
    11761 ccgacacccc atcatccagc agcaggcact gctcttcgtc cagatattcc acccatggca
    11821 gaaaatcaat caccgacgga caggcatggt aaaccttcgt ctcatcctgt cgggtcatct
    11881 tccccggcct tgacagtgcc tttcgtacct gaggggccac tctttctttc atatccggag
    11941 ataccgcttc tccgtcatgc tgcgacaccg gggaagaaac ccgttttttt gtgtctgtaa
    12001 acagggaaaa tcgcatcaca acgcctccgt tcgttcaccc ggcatggcat actgcgtccg
    12061 gctgtaaaaa ggaaatactg tgctgtaccc cggaaccggt gacgtaccgg aagccagatg
    12121 cggaaaaaca tacatcacca tgtcaggatt gggcagccgc ggaaactgct gactgatctc
    12181 actttcctgt gtccggctgt agcttatggc tccgtgcgca tctgcctgct gctccggcat
    12241 tgtcagcgga cgccgcaggg tgtccctggc cgctgtcatg tgccgcgaat gtcctccgct
    12301 tcctttcccc tgccagatct ccagcatact ctgttcaccc ggaggcagaa gctcctcttt
    12361 cgatgtcgag cagccgggca gaataaatac cgccaccatg acggtcatta cagataactg
    12421 tcgtttcacg ggatctcctt acatcagtcc agaccaccgg taccgtggac ttcccccggc
    12481 agagtgaagt cgtaacgtac ccggcgcccc tgatcttcat aatcaatggc cagttgacgc
    12541 gtgatatgca ccgccagcgt ctgccccggg ggaacataaa tggcatcaaa cgtctgcccg
    12601 tatcgctgct tcacccattc cgcggtttca ttcatcccgc cggacagggc ttttccaagt
    12661 gccgcctctc cggcactgcc ggtcagtgtt gatgtgatgc cattgacgtt gttctgtgag
    12721 gtgtactgcc cctcactcag tgcatcaccc gcagcgccgg ctgcagacag ggcaaatatt
    12781 gtcggcagat acgtggaggc attggattta cgggttccgc tgatacacgg gatcccgtca
    12841 tcatccgaaa tccagccaat gcctcctgta ctgctgttat tttttgcctg tgcgttactg
    12901 ctgccgttca cgtctgatga cggcagcgta cgcactgtcc cgtcggcaaa aacaaacgtg
    12961 atgctgttca cctgcccacg gacacaggag agcgtccagt caccactcgc agtaccggac
    13021 accactgcac cttccacatc cggcagctct attccgtttg ccgtcaggtt atccttaccc
    13081 accaggatct tgaacggata gggatccgtc acggttccgt tcaccggaac ccgtcccaga
    13141 agcgctgtca tcgcccggct gcctgccagc gtcgagttct ccggcagggt atacactgcc
    13201 cgggtgctcg ccttctgctg ttcattgctg tatcccttca cctgctgttc ataagccgct
    13261 ttctgacggg tcagtggatt gtcatccaga aaagagactg gaaaacgcgg tccgctctta
    13321 tcccccaccg acgacgcttc atcctgcacc tgatcggccg gtgtcaccca catcagacca
    13381 tccgttcccg gtgaggctgc cgatcctgtc acgctcccca gtccgtcaag gcccagacct
    13441 gctggaatat cgctctcctg tcctccggcg ccggcatcag cactctcctg ctgacgcttc
    13501 agttgctctg tcagtgactg aacctgtgtc attaacgcgt tctgttcatt ccggaactgg
    13561 cgctgcgact gctcataatt tttctgcaat cctgccactg cctcactgac ctgaccggaa
    13621 acatccgtgc cccgcttgcg cagcatttca ttttctttca gcaacttttc attctgtttg
    13681 tccagtacca gttgccgctc cctcacctga ttcatacgcc caatcagtgt gcgcagggta
    13741 tcttcaggtg tgtctccttc cacgcccagg gccttcagtt catccggtga cagtccggcc
    13801 agcgttcctg agctttccgc agacgagggc tccccggtat ctcctccaca gctttttaat
    13861 cccgtcagga tccccgccac caccacacag ggcaccacat acttcacgat cgtattggat
    13921 ttaatctgca tgcttcacct ccccccgtga aggaatgcga cgggaggcag gctccgcgat
    13981 aaacgcctgt tccggccgtt ctgccgtcac cacatagagc gtggtggtat cctcaggggt
    14041 acctgctgca cccagccagc ggtgctggaa agtcgcggca atgaattttc cctgaagtgc
    14101 ccgcggatca aggataattt tgcggcttac cgtactcgtc agtttcagtg caacaactgc
    14161 acgcccaccc gcagcccagg ctgccagtgg cgtgactgtc agtggctctg acggatagag
    14221 tgtggtgatt ttcccgggca gatgtgtatt aaccggccgg atacccggta ccgcttccag
    14281 cgtccgtaac ggtgcgtaca gcgactgtgc cgcatagcgg gtcaacagaa cgggtaaagg
    14341 ctctgcatac ttcaccggct gacggagggc ctgcgagccg tcatccccgc cgggtaccac
    14401 ctttttgtgt tctgcagagc gtgcagacgc cgctgtgcca gcctgactga ccgtactgac
    14461 gcttccctca tacacaatcc gtacgggctc tgtgggccct ttttctgctg ctgtcacatc
    14521 cagcagaata atttctccgc tctccacatc ctgcagttgc agacgcgtct gcggaaaaac
    14581 actctctgct ttcagataga ctgccccacc ggtactctgt accctcaact ttccgttcag
    14641 tgctggcggg aagcccaccc gcacattttt atctgcaaaa ataacccgct cctgtcctgc
    14701 catcagggta acgggtaagg gaatgcgctc ccatttcatc agctcatcag cccgtgcgct
    14761 cagtggtaaa accagcagtg acaggaccat cacaatacta cggtataaac aacgttcagc
    14821 tccgttcatc actgattatc ccccttcccg gctgccgggg aggctgcttc cagccgctgt
    14881 ggtgtactgc tgtagcagtc cagcgccagc ccgaacggat tacgttcagg atccccctcc
    14941 atgcgcacca cctttatcgg ataacggaca agtgcccgtt ttaccggctc agcatgaaaa
    15001 tactcatctg ccaccagatc cagctgtacc tgccagttat cccgcccttc caccgtcacc
    15061 ggatttttgt tgtacctgcc cccgtagcca tgttccggaa tttcatacac cacgcgcaca
    15121 cgctccagca gctcccccgc atcactgcga cggcgggcat cactctccag aatctcccga
    15181 caggatggcg tcaggtaggc agagagcgca tgaattttcg ccgggtagtc cacctctccg
    15241 ttcttcggcc aggcattcag ttgctgaaag atataaaagg caaaggaata aaccgttgac
    15301 ggcgggactt cccaccaggg acgggtactg ccggaacgca gatccggcgg attgtgaatt
    15361 gtcagtctgt ccggtgccgt catccacccg atacagacca gcatcagaat tccggccagc
    15421 acaccgctga aaatacgcag tgtcaggata tgctggtcgc gatcccgcag agcatgacga
    15481 aaacgactca ttttttcccc ctgctacggg tacggcgcag ggaccagcca tgcgcctccc
    15541 ggataaatgt tttccggcca ccaccccatg cactgctttt cttcgccaga tgctgccaga
    15601 gccagttgtc aggttttccc cgcttcagcc ggctgatcca gacgccaccg aaccagacca
    15661 gcagcagggg agtcagcaac atccctgtcg ggataatcac ccagccaagt agaggaatca
    15721 gcggtaacgg ccagatcccc cctgccacag cgcctgtcag ggccgccagc cccagttccg
    15781 gtgtggtaaa accacggaaa accgcaggct ccacgttcag acgatcgggc agaaaacgaa
    15841 tggtctgcat ggggatgtct ccttacagaa tgatggaggc cgacttgccc agcagccaga
    15901 taatggcgac cagcagaaca acaccaacca ccacgatggc accaaactgc ccccatccgg
    15961 ccttcccgtc acgaacctct gagaaggtgg aaagcgccgc ttccgccacc ttcacaaaag
    16021 caactgcact gagcacaaga ccaaacagca caacaccatc ctggagatag cctttgatct
    16081 gtccgaaaag tccaccacct ccgccggatg ttggcggctc tatctgaggc aaatcagcca
    16141 gagcaggtgt gatcccggta cacagaaaca ccgccatggc ataccaggcc tggcgacaga
    16201 tgcgcatttt tttatgcatt atttcctcct gaaacttaaa caagacactg aaatcaaccc
    16261 gcaaacatcc agatactgca gatcagcagc aggacgagtc ggaacataaa ccagccgaac
    16321 acaccatccc ggacatcacc gctacgccag cctgcccagg catccgtcag actccacgct
    16381 gcccagagga acacaactgc cagcagaaat cccagcgtga tgagatgcag cacactgaga
    16441 tccacttcac ctgaagcagc ccggaaggcc gtattctgca gggccgtcat cgccatcagg
    16501 gacgctcccg ccggtagtca ccggtcacgg ctgcggaaga ccggggttgc gcccgggaag
    16561 gggccagata atggtcaata ccgtcacgaa tggtctgcag atctgcacgg gcacgcagat
    16621 aatcaaaaaa gtagcgttcc ccctgtgaag aagcctgcag agaggccgtt ctggcccgct
    16681 ccagcgctgc ctgcacgtgg tcaagctggc gcattacagt ggccagttca tccttttcgg
    16741 cagcctgcgc aggtaatgca aaggacaaaa gaaacagcat ccccgcggca ccggccagac
    16801 ggccggcacg gcagggtgat gccggaaaag aaaacatacg cacctccgtc agagttaaac
    16861 agtgcgtaca ggatgcgtaa ggcagaggac tggatcagcg ataaactcta tttgcaaaac
    16921 agaaaatttt tacgggagaa tacactggtg tggatgaagg caaacaaccc gggcaatacg
    16981 ggcaatacgg gcaatacggg caatacgggc aatacgggca atacgggcaa caataaacta
    17041 tgttctgggg gctgtcagca tagccagttt ctgcatctcg ctgttccaga ttctgttcaa
    17101 ataaatttat tccagcctga atgatttatt cctgtttatt aaaacacggc atcactactg
    17161 tttcccgtta atgacacctc attaacaatt taaagaggaa acacatcagc atggaatttc
    17221 tccgtactgc ccttccgttc tgggccctgt tccttctgac ctgggtttgt gttgtgtatt
    17281 ttttcattta tgaagaacct cgttgccccc cgtggatcta tgacgtcatc agggccatca
    17341 ctctgttcat tattttcgtc aacattgttg tcattccggc gctgtcgtta ctctgacact
    17401 gacgcgcact gaccgtaagg gcgtggcttg tctgccgaca ccctgccaca aaacaagttt
    17461 tgcatcaggg tgtccggcct ctgtggatta taaatatttc ttgaaggatg ccatggtcac
    17521 ggtgatggcc acccccagca gcatggccgc cggccatatc agcaggttcg gatacaccga
    17581 cgtcggccag gacaggtaaa aaatgcaggg aatatatatc gccggtttaa tagacttttt
    17641 tgcatggtga taaacaaagc tggactcata cgcagcacca taccggcgca gatcacgacg
    17701 ccccagtccc tccacaaccg cgatcagtat caccatcaca aacagcggaa cagacaacac
    17761 caggatcgtc acccgtatca gtgtgaccac cgtgacatat actgtggcca tcaggtactc
    17821 acacagataa tggctcagcc agcgccccac atcctcactg tactgtgaca cactgttacc
    17881 caccacggca ccggcatttt gctgcttcag ccacgacaga aaacctgttt ccacaaacag
    17941 ccactgatac gccgttgcta tccattctgt caccgttacc gatggtgtcg acagcagcag
    18001 actgcgggtg aattccgaag acagccaccc cagttctgtc ttcatcactg cctcactgtg
    18061 tgctgccccc tgctccggcc agaaaaaagc aatcccgata tactcaacca gcaggctcag
    18121 cagcagggag gaaaacagga tcccgaccag catccacggc cagccccaca acagattcca
    18181 gagcagtccg tgctgacgtg gcggagaggg ttgtgctgct ctgttcacct cacgacgggg
    18241 ttcagccatt ctgctctcct tccggcatgt tcacggaggg agtctcattc caccatccct
    18301 ctccggtgtg gtaattacgc cgcatggcct ctgcaatttt tgcaatactc tccggcatca
    18361 gcgcgtcatc tgcatcagtt gcaggcaggg gcatacggat tttccacaac tgtccccctt
    18421 ccagcagagc aaaggcctgt cctttcggca gggtcacgat atctgccggc tccagcaaag
    18481 gcaccttcac agtaccaaca cgatcctggg tactggatgt gaagtcctgg ccggtcgtga
    18541 catctgcggt atcctgatga ccggaaacca gcgttttggt ataaatttcc acctgggaca
    18601 actgggtcgt cagtaactcg gccgtccggt tctccctgac ccgcagcata atcatgttat
    18661 taaagttacc ctgcacctgg gccgttttgg cagcactacc ggtacgggcc tcaatatcag
    18721 aagaggtctg cgtgtaagcc gtcacctgca taccggcacc gcctcccttg ttgattaacg
    18781 gaatgaactc atcgcccatc agttcattaa actcatcaca atgcaggtta accggatttt
    18841 tggtaccatt ccctcccgga agaccggcat tcattccgtg tttgtagata tgcccggcga
    18901 cactcaccag atccgcaaac atggagttac ccacggcact ggccacctca ctgtcactca
    18961 gcgcatcaag accaatgtac accaccgcct ttttacgtat cacctgctcc cagtcaaaca
    19021 cgggacgggt atcgctcata tccaggtagt caggtgccag cagttctgcc gtctttcctg
    19081 tggtcagttt ttccagcaac ggcaacaggg atgccacaat tttgtcaaaa taggtacgat
    19141 cataacgtac cgcgcttcgc agaccatcca gtatcggatc atagagtttc tttccggctt
    19201 cactgctgag agccacctca atggcccaga tacgcacagc atccggctgg ttctgcatgg
    19261 tccggggaac atccgcttcc cccagtacat tcatgttatt cagaatggcg ttttccagtt
    19321 ccggcatctg ttcgctgatc accttaccag cataacggat ataaaggtcg gcaatattat
    19381 tcacataacg cataatcagc gtgtaatctg ggcgctcccc cagtgccacc agtgcacggg
    19441 caacaatgtt gacaaaccgc catgcaaact cacggaaggc cgcactgtta ccttcaccgg
    19501 ataactgacc ggccacacgg gaagccacct ccgacacacg accaaaacgc cccacggcgt
    19561 tataacgcgc agagatttcc ggccagccca gatgaaagat ataaagctca tcgcccctgc
    19621 cggcacggtg tgcttcagcc cagacacgca gcaacagatc cgcatcccct ttgggatcaa
    19681 agacaatggt gacatctccc cgacgaatat cctgtgtgac cagcaactcc gccagccggg
    19741 ttttcccgac acgggtggtg ccatagacaa tggtgtgccc cacacgttca cccagtgcca
    19801 gcgtcacatc ctgctcatcc ggctcaatac catgcagggc aggatttcct cccaccggcg
    19861 gcaggggacg gacgggattc agcggcgagt cacgacgaag aagctgccca atccagggaa
    19921 gactgtgctc cgttttcatt tcaagccagc gcgctgtccg gtacagactg cccggctgta
    19981 gatagtgtgc aacctccggt ctcagcgtgt cctgaaggcg ctgggtgtgc ttctgtgtcc
    20041 agcggaagcc gcgtcccaga aacagccgct gacggctgac cggaatttgc cgggaactca
    20101 tcacataacg gggcagccgg cgcagattgc gacggtaacg gatgatcttc attccctgcc
    20161 ggaaacggac cactgccaga acgccaaagc cggcggctgt gacatagctg accgaaggcg
    20221 cgagcgccac cgcccagggc gctgtcacac agacaccggc cgccgttgct gccaccacgg
    20281 cggtattcag ttccacagcc ggacgcagca gagcctcaat cacataacgg ttactcatga
    20341 ccgggtacct ctctgtaaaa gacgccgcca ccatgagcgc ggcaacgctt tctccggtga
    20401 tgctgacagt gcctccagtc tgcgctgtaa caccatcacc tcccgccatg cgtctgccgc
    20461 attcagcagt aatgccttat acaccagtgg cccgtcatgt ccgccggcat gcacatcatc
    20521 cactgtcaac agcgcctctg ttctgaaacg gtccactttc cagcaggcca ggtcttcgcg
    20581 ggaaacacac tgccgccgga tctgcagacg aagatacaca tcatgccggg acaacggttc
    20641 tgccaggttg tgaatttccc gacaggcaga ccggaacgcc tggtctgtgt gataaatact
    20701 ctgggccaca ttgtgaagct gtgtgcgccc ctccccctgc aggtggaaca gaaagcgggc
    20761 cagacagctg cggtgattac cgtcagcact gacataccag tgaatgccat ccgtgctgac
    20821 aaaacccatc ccggcatgct gttgctctgc ctgcgtgtaa tatcccggat tcttttcaag
    20881 taacgggata ttgatgtcca tgcgtttacc acgctccagc agctccagcc agctcattcc
    20941 ggcatactgt ggatggtcag tccccacaat gcggtacaca ttgatactgc cctgtcccgt
    21001 ccagtagtgt tccgtaataa aatccgacat tgccgcaaac tctgcggtat cccatgcaac
    21061 cagttgttca tgtgcccagg ccggagcatt cgcctgtatc cgctctgtaa atgccccctt
    21121 cattcagctc ttctctcctg cacttttgag ataccgatac cgtcaggcca cggcataaca
    21181 gccgggcccc ccctgccttc cccggatacg gctgcagaac cacaaccacg aaacagaatg
    21241 actgacgaga cgccttccat aaacaaagcg cccagcaaca gaaccactgc ctgcattttc
    21301 agttcacgaa tatcctcagc aacagcctcc ccgaaagaaa tcgctgttcg cggacattcc
    21361 tgctcatcga catacatacc tgagacggat atgacggagg aacatttttg taccatcaca
    21421 tgcccttcag gcgcatcacg ccagcgggat gactccgtcg cataacgctg tgtcatgcct
    21481 gcctcaccac cactcagatt gttccagaca tacagcatta ccagacatcc tgccagcatt
    21541 gacggccaga caatcagaag ccggagcagc agcataacaa accggtacag tgtcataatc
    21601 atgatcttcc tttattactc agtaaggtgc cattcagcag ttgcaacaga cgttgtccac
    21661 tgatcagctc aatatccggc cacagtgtca gccgtgcccg gctgatgttc cccgtacgcc
    21721 ccgtatggat aaaaaagccc ctgcatccct tccggcgcag cagttcgcca aaagcatcaa
    21781 tatgtgccgg acgaatggtt cttccgtaac gtttagcctg tatcagccag cgttcaccgt
    21841 taatcaccac ctgaccatca agaccaccat cgccgctgta tgaggggtta cggataaccg
    21901 gcatcccctg ttgctcaaaa gccagcagca acagttcctc aaacacatac ggactgattt
    21961 tgcgcagata tttcagccgc cgggcatcac cctgcaattc gttcagccgc ttcattaccc
    22021 gtgaggccgt ggcacgataa cgctgatgcc ggcgtgttct cacacacgct ttttttcgcc
    22081 ggctgcgcca cagtgccggc cccaggacaa cagccagcag gctgagaacc aggcaggctg
    22141 tcagtaccgg agaagcagag agcgcagcag aagtggcaga cagcggcatc atggtgcagg
    22201 tacctgtgac aggccgttgt ccgttatcag caccgggtaa tgacttatct gtaacctgcg
    22261 ggccagtaca gaaccggatg ccggaaccat caccaccccc ggtgccagct cacgcagtac
    22321 gcgcagactg ctctcatccg ccacattcac taccattccc gccgccttcc gctccctgag
    22381 tactgctgca ttcatctgca gccatgtgcg ggaatgggca tcatcgccca caagaaaaag
    22441 tgcaccgatg cccggtaact gaagccggcg cgccacaacc tctccgggtg acatttcagg
    22501 cgtactgacc ggtaacattg ccgcctctcc ctgcggaaaa gagggtgatg gcacaactga
    22561 agcgggaacc ggtggctgcc ggtttatcgc ctcaaaaaag gggctggcat ccctgccgcc
    22621 caggtctgca atcactttca gttctgcgcc agctgttgta cagaacagca gcagactcag
    22681 tacataaaac tgtctttctc gtttcatttc tgctcctcct gtggttcagt ccagatataa
    22741 cggggggatg gtgtcagcat ggcggtcgct gccacatgca catcagacgg attcaggaaa
    22801 ttatcccggt ggagccgggc aagatgccgt ctgaccacgg cggtataacg tgcggccggc
    22861 gcaccacctg ccgggtggtg ataacacccg gccacctgca gccagctgac cggatggcga
    22921 cgccggcatt cctgcagaat gaccgctgcc gtattcagac tggtgtaagg atcaaatgcc
    22981 gcccatgtgg acgggaaacg atgtccattc cagcccagat tgacctgtgc tatcccgaca
    23041 tcaatacgtt ttgccggata atgtttcagg aacacctgca atgcctgcca ggcctccaga
    23101 cgtgtggcat aacggtaccc ttttcctgcc acatttattg tccagggcca gggtcgggcc
    23161 acgtccggct gacgaaaatc acgacgccgt tgtttcagta ctccggacat cgcttccgga
    23221 gacatggacg tttccgtcag ggaaacggca tacaaaagct ctgcgggaat accatgggca
    23281 cgggccaccc gcacataccc gtccggtacg ttctgaacgg cagcacgaca gacaccactg
    23341 caggccacca tcagcagaac cgtcagaatg ccgccacgcg ccatccgtca gccccctgct
    23401 gcagtaccac cggcattttt ccttcaccaa aacgcagcca gcgtccggca tcatgattca
    23461 gggtgatatg acggctgcga actttctcca caggtatgct gtgcgcatgc gcccactgac
    23521 gcagaatctc atcctttccc tgactgtcga caaggtagat atcaacctgc cgtcccgtgg
    23581 ccagtacgga ggcaagacgg gcatcacagg tgccacattc cttcgccttc acaaacagcg
    23641 ccagccgtcc ccccgtatct ccggtaagtg ctcctgtttt tcccatactg acaggcaaga
    23701 tactgggata caggcgcttc caggccgcat tcacctcctg ctgaaacgcc agctccttct
    23761 ctgttcgttc atattcagcc ctgacccact tttcggccca gacacgtcgc tctgccggcg
    23821 ttctggcctc tataccgagt accgtcagcg gatcaagtcc gggagactgt atcccccggg
    23881 ggccggacat cagtgactga taacgccggt aatcctcctc actcaccccc cactgcccgg
    23941 cctgctgctg aagtccggat gtggtactct gcgtcaccac cgtaccggtc gtccgttgcg
    24001 cctctgacga catattcgtc tgcccggcgg actgagccag aacggggcac agtgccagca
    24061 gatacagtat ttttcgcttc atcatctact ccaccctgag caccgtctga cgaccattca
    24121 cccggaacgt tgcttcacca tggccggcat acacaagcgt ccacccggcc acactctctc
    24181 cttcacccag cagagccacc tgagagaggc tggtataccc ccggggagcc acggctgcac
    24241 gggtactgcc accgcgcagc tctgtcccgg tcagcacaaa aggagcctgc cgaactgtat
    24301 gaactgcgcg ggatttacgg gaggacgtca ccggagcggg ctttttctcc tccgtttttt
    24361 tcatccccgc tttcctgctg tccggctgtg acggtgactc cggtactttc tgcattttct
    24421 ccaccagacc gctgagtgta tccagtctca gggaaagctt ttcccgggca gactcaatct
    24481 gctccagcat ctccggcaga ttctccagtt gttccttcca ggatgctgtc agcacgggct
    24541 gctgctccag gactgccagg cgcttttcca tatccttcag ttgcttctcc gtctctgtca
    24601 gtttcattct caggggcgcg agttctgacg cagaaagcat ttctcttctc tggccttcca
    24661 gggtcgtgat ccggctgtca agaccggtca taagcatcag tacgccatat accactgccc
    24721 ccatgaccag ccccacagtg acagacagta tcaacacgcc ggggaaacga ctttttcttc
    24781 gctccggcga cataccagac gcaccctccg gtatctgcgg ctgtttctca ctcatttttt
    24841 cagaaaccct ccggttgcct tcacacccgg ttcacgaccc acaaaaggaa cggtgaccgg
    24901 aacaggagcc agctggctga ccggtaactg gtaaccgtca cgcagactgt ggcagaccac
    24961 cctctgcaca tcatccactt ccagttgcca tgccggcccc gccattaact gcagggcagt
    25021 acgcagtctc agtggtcccg gctgatactg caccgccggc agaggctgac ggtacagcac
    25081 gccgttggcc ggtccggtgg cgcagagcgt gtaaccggac tgtttcaggg cataacgcat
    25141 ggcatcggcc accgtgggtt tcagggagga gggaatgcga atgtcgatca cctgagaaag
    25201 tggatcacgc tgtacagcct gcggctctgt gctgaccagc aggtaacggt catagcgcac
    25261 cacctccggt gcctgagaat acacatccgg gctggcaggc tgtacattac ggtttactgt
    25321 gaccgacggt accggcatgg ccgcatcccg ttgctgtagt ttctgagtgc tctgacatcc
    25381 cgccatcagg aggcagactg tcagtccgga caggataaag cgttttttca tcgggtattt
    25441 acctctgcag tgataaatca gaggtcaatg tgcagaagaa gacggggctg gctcaatgaa
    25501 caactcatta cccgctggcg aaaaaaaacg cctgcagaca tgcaggcgct ttgttgacag
    25561 gaaagaaagc aacataacaa atatgtcaca atcctgcggt taacataaat taccgattac
    25621 cggagagcat tacgcaaatc atcatccgag aactgcagta tttgtttgat tttctctgac
    25681 gatataccca tctgctgcat gcgacgggcc gatgcgtaat tctcctgaag agccttttct
    25741 ctctcctcct ggcggccctt ctcgatacct tccaggcggc ccttctcaat accttcctgg
    25801 cggcccttct cgataccagc cagttccagt cgttcagcga tagtcattag atcatcctca
    25861 tgtcttggca gacgttctgc catcgcccgt ataaattcag gtgaagctga atcacattct
    25921 ttcaccatat agttaaacaa tgtcaccacc tgagtgtgtg tttcctgtat ctgaatcaac
    25981 tgtgacaaaa agtcaagatg aaccatcaga tccactttct tctgccggag cttcatcata
    26041 agcatcagcg cagccatctg cttatagcga cggataccct catcatcaag aagaccgaca
    26101 tcaatcaccc tgggtggcct gccttctcca taaagctccc gaccaaacgc cggatcatcc
    26161 agacagtcag gccagttcat gctgtatgga taaggactgg gtacaccatg gtaaaaaagc
    26221 accggtatca caacgggagc acacgcatgt gctttcaggt ggcgatgtat caccgccaga
    26281 acatagcgca tcattcgcag ggccatatac ttatccgcct tgctctggtg ctctgtcagc
    26341 gtataaatgt atccatccct gccatctgtg gttttcatac tccacagaac atcacaggca
    26401 aactggcgaa gatccggatc cacaaacgtg gtcggctcca gttgcagggt gttcatatcg
    26461 caccggttca gttgttccgg gggcaacgcg cctttcagga aatccctcgc aacagaagga
    26521 cattccatca tcgctctgaa cgccgcatca tgtggcgtgg gggtagtggg tgtgttttca
    26581 ttcacaaccg cctccttgaa ttaaaagtgg ggcggtaagc cccgtggtta acatcagatg
    26641 acatggataa tccgtcgttc aacgccatac ccacgcccca taaaggggat atcatcgtca
    26701 aagtccatcg gcggcattcc cccgcttcct gaggaagcct gaccgttttt tcccgttgat
    26761 tgttgtgcat tttgtggtgg ttgtccccat cctccctgag caggagcaga tgaaacattt
    26821 ccaccaccct gctgctgacg cggagacccc agcatctgca gggtaccatt gacactcaca
    26881 acaacttccg tggaataacg atcaacacca ttctgctgat cgaaccactt ccgggttctt
    26941 aactgacctt caatatacac ctgtgaacct ttacgaaggt attcaccggc aatctcagcc
    27001 agttttccgt aaataacaac acggtgccat tcagtgtgtt ccttcatttc accgctctgt
    27061 ttatcacgcc aggtatcagt ggtggccagc gtcagatttg ccactgcacc tccgccgttg
    27121 gggaaataac ggatttccgg atcctgtccc agatgaccaa tcagtatgac tttattcact
    27181 ccacgcgatg ccataacaac tccttatcct cacacacatt cacgctgagt ccagatgccg
    27241 ttttccagag cccggactgc ctgctcataa tttctgaaat actcttcgga ttcccgggac
    27301 acaggccctt cgtcgcactg agtaccaata taataaccac gagcactgct gaggatctgc
    27361 agaggtaagg tatagcccgc tttggcggcc agaattccgc ttgtcggttt cataacatgt
    27421 ttccttaaag ttaaacgata agaaaacaga cctccctgat ggttggtctg ttttcatcag
    27481 ggaggttgag ttgaaaaata cgatcagaaa gaattttctg cgtattcccg cggggtattg
    27541 ccattctgtg gcggtatgga ttccgatttt tcagtcttat aaaccatgtc ctggcctatt
    27601 ttgatccact ccaccttgat caggcgggct ttcagactga cgcgttgctc accggcatgt
    27661 tcaccccggt taagggtaaa aatgtcagta gaggggttgc tcatggtaaa accaatcagc
    27721 accttgcgat cttcatccac cgctttctgg cagcgggcaa tcagggaagt tgcctcttta
    27781 ccggcaaccg tcacatcaaa acgcacatag gacgggctgt ccgtcggacc actcagtgca
    27841 ttcacaacac aactgagaaa tgtcccgttc tgatgattaa cctgacgaat gttgctgaga
    27901 taccccaggc ctttcagtgt cagattgaag tactcagttt tagcgtttgc agaagaagta
    27961 tttgcagtca taatgatatc tccattctca gtatcggaaa gacagagaac atcatcatcc
    28021 ctgacgggtt gtgatattcc cgtcagggag caggaacaga actattccgg ctgactcagt
    28081 aacagttaaa gaaaaccttc atcgtcatga ctgacgatat ctgctctcaa aggtgggcag
    28141 atttaccacc accagaaaca tcctggctgc acatcccctt tcaggttacg ggggtgttga
    28201 tcacccgaag gcgttcctct cagatcaacg gaacattggt gcccaccgaa gcgggatgtg
    28261 gtcacccgaa ggcgctcctc tcagaccact tatggagcat tggtggtcgc cgaagcgaca
    28321 tgtagccacc cgaaggcgct cctctcaggc cacttgtgga gcattggtgg tcgccggagc
    28381 gacgttatgt gcccggacag actgaaatca ctgatggaaa aagtcaacag ctaaccaaaa
    28441 acaaaaaagc accggcaagc cggtgcttca gggggatacc aaatcccggt gttgattctc
    28501 atcttctccg gagagagcga ctcccggcac ggttttcacg gagtgcatgt gccttgtaac
    28561 cccgccgtgt gcataacggg ttaccggacc tctcagggct gtcagcccca ctgtggtcaa
    28621 cagggtgata ccagaactcg cctgcaattt taattcagtt tgtctccgac atcaccagat
    28681 ttttgtttat gagaaaaatt aatttttctt ttcctgcccc tgctgtttcc ctgctcacca
    28741 gcaattcccc tgcagtcggg ataacgggag catccccaga aaaaaccgct ttttcccttc
    28801 cgtttcaccg tcggaccgtt acagagtgga catgaaggac ctgattccac tgacagatta
    28861 accgtctggt tacgtccgga taacaccagg tgcctgatcc actgatgctg tttctccata
    28921 aacgcctcca gtgagatttt cccccgggca acatcatcca gcgcctgttc ccataaagcc
    28981 gtcatacccg gatttgtcag agagtccggc aatgccgcca ccagttctct tcccagttgc
    29041 gtggaacgga tatgttttcc cgctctctca atatagttgc gcgcaaataa cgtttccagg
    29101 atccccgcgc gtgtggcttc cgttcccagt cccgcattct ccctcaggat ctttttcagc
    29161 gccggatccg ttacaccaga cgaagcattc ttcatcgcag taataagaca gccttcagta
    29221 aaaggacggg gaggtacggt tttcagctcc ttctgttcag caccatccag agcacagagt
    29281 tctcccttta tcaaaggggg caatatgttt ccactttcat cactgtcagg cttttcttca
    29341 tctgcatgat tttccggtgg aaataactgt ttccacccga taatcacatt aactcttccg
    29401 cgggaacgga ataactgccc cccaatcaca aatgttgcct cagtaacatc agcttcctgc
    29461 tccggcagaa actgtgccag atagtgctga cgaatcagtt gatatacctt cagttcatca
    29521 ccgttcagtg agctgaggtc aaaaacctgc cgggtcggaa taatggcatg gtgagccgta
    29581 atttttttat cattccagat acgggatacc cgtcctgatt ccagttcagc cagttgtgga
    29641 agcacggacg gatctgaacg cccgacagcc tcaagcactt cagaactctc ctcatgcatc
    29701 gaggcaggca gataaccaca gtctgtcctg ggataagttg ttgctttgtg agtctcataa
    29761 agggactgtg cgatatccag aacggtctga gcccccatgc caaacttccg ggaacaggct
    29821 tcctgcaacg ttcccagcga aaaacacaaa ggtggcggtg ttttctccct cttctgtaca
    29881 acatcctgta caacagcaga ggactgctga cggcacagac cgagaacagc accggccgcc
    29941 tgcacattca cacaacgtcc ctcatcatcg cagtactgct cagctgccac ccagttactg
    30001 cggaaacgga gcccctcctt ttccagccag acaatcacct gccaccagga aaccggcaca
    30061 aatgtggcga tctcctgatc ccgtctgacc accattgcca gcgtgggtgt ctgcacccgg
    30121 cccactgaca aaaccccaga aattccacgt tcccgtgcct tcagggtata caaccggctc
    30181 agattcatcc ctgtcagcca gtcagcccgg gcacgcccct taccggcttc actgagaagc
    30241 gcagtctgtt cgcccgggag aatattattc agcgccttcc tgatactggc ctcatccagt
    30301 gcagataacc acagacgcct gacacgccct ctgtactgac agtactccag caactctctg
    30361 gctatcactt ctccttcacg atcggcatct gtggcaataa ccacttcaga agcgcgtttt
    30421 aacagccccc ggatcacaat aagttgtttt ttcaccttcg gcttcaccac catctgccat
    30481 tgttccggta tcagcggcaa cacatcagaa cgccatgggg aaccgaactg ttcaccgtaa
    30541 gcttccggct cagccagttc cagcaggtgt cctatcgccc aggtgacagc aatattgtca
    30601 tctccgatgt atccgtcgcc ccgcttactg agcgccagtt cacgggcgat atctttcgcc
    30661 tgagaaggtt tttcacacag aaagagttgc atactcaccc cgctttaacc ggtggtgaat
    30721 aaacagaacg tttcttaccg ctcatcacgt caggatccgg ctcccccatg cggttaaccg
    30781 cctcctttcc acgggctgtg ccggacaaaa tatcagcacg tgtaacactg actgatttat
    30841 aattttgaat aatcccgtaa atcctgcgta ccgcataccc tccctgattc aacaactcat
    30901 cccgttttgt gcgggaaata agtccgtaat gaaatacctg aaataccttc atgaccagtc
    30961 gatcatatcc gatcagaagc catacacatc gataaccgac cggagaacta ctgtacacgc
    31021 caatatttac cggcgaagag gatttaatat cagaaaggga gatctgtccc ggcaggtttt
    31081 taaggatccc gtcaagccac gcaagatgct tttcaattgt ttcagttgcc ttcgtcaggg
    31141 cattttccag cctgactaac atcatatctg cataaggatt atccctggct gaatctctgg
    31201 ttgcctgacc cgcaaacgca atagccctgg gcatactgat gatgtcaggc cgcttttcct
    31261 gtttttcctc cttctgatta ttcctgcgtc cctcccagag acggatcgca tatcgggtat
    31321 gaagggcaat agtcagcgat gactgcagag caccaacttg ttctgatttt ctgacttctc
    31381 cggttttctc ctggggcatt gtgtttactc caaatgattt catgaccaga agatagtccg
    31441 caggttgcgt attaccgaca acaaaaaaac aaatcaggca gaatatacat ttaacgccga
    31501 ttatttttaa tcgctgttga cactggcaac accgctcact ttttaacctc tgttgccact
    31561 ggcagcactg ttcacttttt aacctctgct gccactggca gcactgttcg ctttttaacc
    31621 tctgctgcca ctggcagcac tgttcgcttt ttaacctctg ctgccactgg cagcactgct
    31681 cattttttaa tctctgctgc cactggcagc actgttcact ttttaacctc ttct
//
LOCUS       DAZGXZ010000050        31577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000050 DAZGXZ010000000
VERSION     DAZGXZ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.050"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(117..413)
                     /gene="yaaX"
                     /locus_tag="ACHKJF_002389"
     CDS             complement(117..413)
                     /gene="yaaX"
                     /locus_tag="ACHKJF_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP0949736.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(627..1913)
                     /gene="thrC"
                     /locus_tag="ACHKJF_002390"
     CDS             complement(627..1913)
                     /gene="thrC"
                     /locus_tag="ACHKJF_002390"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP0949737.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1914..2846)
                     /gene="thrB"
                     /locus_tag="ACHKJF_002391"
     CDS             complement(1914..2846)
                     /gene="thrB"
                     /locus_tag="ACHKJF_002391"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP0949738.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2848..5310)
                     /gene="thrA"
                     /locus_tag="ACHKJF_002392"
     CDS             complement(2848..5310)
                     /gene="thrA"
                     /locus_tag="ACHKJF_002392"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP0949739.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5336..5455)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5391..5456)
                     /gene="thrL"
                     /locus_tag="ACHKJF_002393"
     CDS             complement(5391..5456)
                     /gene="thrL"
                     /locus_tag="ACHKJF_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP0949740.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5670..6356)
                     /gene="yjtD"
                     /locus_tag="ACHKJF_002394"
     CDS             complement(5670..6356)
                     /gene="yjtD"
                     /locus_tag="ACHKJF_002394"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0949741.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDIDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6756..6896)
                     /gene="yjjY"
                     /locus_tag="ACHKJF_002395"
     CDS             complement(6756..6896)
                     /gene="yjjY"
                     /locus_tag="ACHKJF_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP0949742.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6992..7708
                     /gene="arcA"
                     /locus_tag="ACHKJF_002396"
     CDS             6992..7708
                     /gene="arcA"
                     /locus_tag="ACHKJF_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP0949743.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7768..9120)
                     /gene="creD"
                     /locus_tag="ACHKJF_002397"
     CDS             complement(7768..9120)
                     /gene="creD"
                     /locus_tag="ACHKJF_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP0949744.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGELSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQITDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9178..10602)
                     /gene="creC"
                     /locus_tag="ACHKJF_002398"
     CDS             complement(9178..10602)
                     /gene="creC"
                     /locus_tag="ACHKJF_002398"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP0949745.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNSADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10602..11291)
                     /gene="creB"
                     /locus_tag="ACHKJF_002399"
     CDS             complement(10602..11291)
                     /gene="creB"
                     /locus_tag="ACHKJF_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP0949746.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11304..11777)
                     /gene="creA"
                     /locus_tag="ACHKJF_002400"
     CDS             complement(11304..11777)
                     /gene="creA"
                     /locus_tag="ACHKJF_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP0949747.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11988..12857
                     /gene="robA"
                     /locus_tag="ACHKJF_002401"
     CDS             11988..12857
                     /gene="robA"
                     /locus_tag="ACHKJF_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP0949748.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12854..13501)
                     /gene="gpmB"
                     /locus_tag="ACHKJF_002402"
     CDS             complement(12854..13501)
                     /gene="gpmB"
                     /locus_tag="ACHKJF_002402"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP0949749.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13553..14074
                     /gene="yjjX"
                     /locus_tag="ACHKJF_002403"
     CDS             13553..14074
                     /gene="yjjX"
                     /locus_tag="ACHKJF_002403"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP0949750.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14159..14485)
                     /gene="trpR"
                     /locus_tag="ACHKJF_002404"
     CDS             complement(14159..14485)
                     /gene="trpR"
                     /locus_tag="ACHKJF_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP0949751.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14575..16512)
                     /gene="sltY"
                     /locus_tag="ACHKJF_002405"
     CDS             complement(14575..16512)
                     /gene="sltY"
                     /locus_tag="ACHKJF_002405"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP0949752.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMILSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRHGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16723..18390
                     /gene="ettA"
                     /locus_tag="ACHKJF_002406"
     CDS             16723..18390
                     /gene="ettA"
                     /locus_tag="ACHKJF_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP0949753.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18697..19929)
                     /gene="nadR"
                     /locus_tag="ACHKJF_002407"
     CDS             complement(18697..19929)
                     /gene="nadR"
                     /locus_tag="ACHKJF_002407"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP0949754.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19950..21332)
                     /gene="radA"
                     /locus_tag="ACHKJF_002408"
     CDS             complement(19950..21332)
                     /gene="radA"
                     /locus_tag="ACHKJF_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP0949755.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21381..22349)
                     /gene="serB"
                     /locus_tag="ACHKJF_002409"
     CDS             complement(21381..22349)
                     /gene="serB"
                     /locus_tag="ACHKJF_002409"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP0949756.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22455..23099
                     /gene="ytjB"
                     /locus_tag="ACHKJF_002410"
     CDS             22455..23099
                     /gene="ytjB"
                     /locus_tag="ACHKJF_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP0949757.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23127..24143
                     /gene="lplA"
                     /locus_tag="ACHKJF_002411"
     CDS             23127..24143
                     /gene="lplA"
                     /locus_tag="ACHKJF_002411"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP0949758.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQKCEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            24175..24438
                     /locus_tag="ACHKJF_002412"
                     /pseudo
     CDS             24175..24438
                     /locus_tag="ACHKJF_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(24599..25318)
                     /gene="deoD"
                     /locus_tag="ACHKJF_002413"
     CDS             complement(24599..25318)
                     /gene="deoD"
                     /locus_tag="ACHKJF_002413"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP0949759.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25398..26621)
                     /gene="deoB"
                     /locus_tag="ACHKJF_002414"
     CDS             complement(25398..26621)
                     /gene="deoB"
                     /locus_tag="ACHKJF_002414"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0949760.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26673..27995)
                     /gene="deoA"
                     /locus_tag="ACHKJF_002415"
     CDS             complement(26673..27995)
                     /gene="deoA"
                     /locus_tag="ACHKJF_002415"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP0949761.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28122..28901)
                     /gene="deoC"
                     /locus_tag="ACHKJF_002416"
     CDS             complement(28122..28901)
                     /gene="deoC"
                     /locus_tag="ACHKJF_002416"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP0949762.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29159..30709
                     /gene="yjjI"
                     /locus_tag="ACHKJF_002417"
     CDS             29159..30709
                     /gene="yjjI"
                     /locus_tag="ACHKJF_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP0949763.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30681..31544
                     /gene="yjjW"
                     /locus_tag="ACHKJF_002418"
     CDS             30681..31544
                     /gene="yjjW"
                     /locus_tag="ACHKJF_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP0949764.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         7498 a         8698 c         7962 g         7419 t
ORIGIN      
        1 caggcagaga aaactggccg attttctctg cactggaggg ataatacaag gcctgataag
       61 acgcgccagc gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag
      121 cggtgatgtt tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg
      181 cgcggcggtg gcggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt
      241 ttccaccagc cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga
      301 tcgcctatct gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg
      361 ggagcgacca gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac
      421 tccttttatc attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt
      481 tgttattctc cgcaatccta atctctaagt taccgcattt attgagaatt tctccttttt
      541 ccctgtcgtt tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc
      601 ggcctgattg agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca
      661 gcaaaatcgg cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct
      721 tttggcagat ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga
      781 tgcgcggtgc cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga
      841 tacgctacgg cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc
      901 tgttgcgtgg tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg
      961 cggcggaaca actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg
     1021 gataacgtcg cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg
     1081 gtatcgttca cgttggtcgc agcaataaaa cgtttcaccg gcagaccgag tgacttcgcc
     1141 agcagacccg ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg
     1201 cgcgcttcct gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa
     1261 cggctgatgt taatcgagtt agctgagttt aaccccagcg ccactttcag ttcttcatca
     1321 tcaaacgcct gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt
     1381 tcgatattgc cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt
     1441 ggatagagga taaccacttt cacattcggt aaaccgtaga aagcatgagc cactgccgct
     1501 ccggtatcac cggaggtcgc ggtcagaatg gtcactggct tatcgcccgc aatatgggtc
     1561 agcatttgtg ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac
     1621 aattccagac aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc
     1681 gcgcgcacgc gctcttccag gatttcctgc gggatttcat caccaataaa cgccgagagg
     1741 atcttcgcac tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg
     1801 aattccggca ggtcatgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg
     1861 gcttgcgcaa agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt
     1921 tccagtactc gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt
     1981 tgcaggtagt tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat
     2041 agagcgaaca aggtcgggcc ggagccggag ataccgctcg ctaccgcgcc gatttccgcg
     2101 accgcctgcc gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata
     2161 acatctttca tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag
     2221 ccagccagat gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata
     2281 gccctggctt ctgccgtcga gactttaatc cccggatacg ccagcaccca cagccactca
     2341 tcaaaccctg gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca
     2401 ccaagaaaac acggtgccac gttgtcgtaa tgaatgctgc cggagattcg tccttccagc
     2461 tcgcccatca aagccagcaa acgagtgtca ttaagcggct tgccacagtg ttcattcatc
     2521 gccatcagcg ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gattggcata
     2581 ttcttttcca gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc
     2641 cagcactgat aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg
     2701 aggttgttga gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca
     2761 ccatcaacag gtgtcaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg
     2821 gaagccgggg cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag
     2881 cagatcggca aagacaccgg cagctgtaac gtcattgccc gcaccatatc cgcgcagcac
     2941 caacggcagc ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac
     3001 tttgaacagc ggatcattac catccacttc ggcaatcttc acgcggcagg caccatcttc
     3061 atcaatattg ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc
     3121 ggcaaagaga tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcagc
     3181 gttaaactct gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc
     3241 cgtttcacga gcgagaatca atagcttacg cgctacatcc ataccagaaa gatcatctcg
     3301 cggatccggt tcggtataac ccatttcccg cgccagagta gtcgcctcgg agaaactcat
     3361 gccttcgtct aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt
     3421 catcaattca tcaccagcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc
     3481 cccaacgttg gtgtcataga ggaatttacg ccgcgatttt tccgccgcat gacgcaacag
     3541 atggtagtaa tccatcgacg aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc
     3601 ttcgcgcaag aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat
     3661 gaccgggttc agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg
     3721 ctctttggct tgcgccagtt cttcctgcca gttttccaga tttaggccat gcacattggt
     3781 gagcagtgcc ttcgagttgg caacaccgca gacacgtaag tcgatatgtt tattcttcag
     3841 ccagctttgt tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat
     3901 cacaaacact tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc
     3961 agtggtcgca tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc
     4021 aatggcgaca atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc
     4081 acgcaaggtg cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc
     4141 cagcggctcc agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc
     4201 agcccgcaca cagtcgcttt gcggaacgca gaaactgata ctgtattcgg aagatgattg
     4261 cgtaatcagc accacggaaa tacgggcgcg tgacatcgcg gcaaagacgc gcgccgccat
     4321 gccgaccatt cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt
     4381 ggaaatgccc ttgaccggta attcgtcttc atcacgactg gcaccaatga gcgtacctgg
     4441 tgcttgagga tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt
     4501 aatggtgcgg gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta
     4561 ggacatcgac ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc
     4621 gtcaacgtcc gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc
     4681 ggagtagtcg gaaccgttgc gtccaagtac caccagttcg cctttttcat taccggcggt
     4741 gaaacctgcc atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt
     4801 ggactcagca atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac
     4861 cggatcgata acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat
     4921 cgacattttc tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa
     4981 caaactaatg ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt
     5041 caattgcgcc agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa
     5101 aatacgttcg gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat
     5161 catcgccacc aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc
     5221 ctgcctggca ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac
     5281 tgatgtaccg ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa
     5341 aaaagcccgc actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca
     5401 ccgttacctg tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat
     5461 gttgtgtact ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt
     5521 agtgacctaa gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc
     5581 tatcagacac tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa
     5641 aagctgaaaa atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc
     5701 agcaaacggt gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgttgtaac
     5761 cagtcgacca gttttatgtc atctgccact gccagagtcg tcagcaatgc catgactcgt
     5821 tcgcgtaaag cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt
     5881 attaatgttg ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga
     5941 taatccgcca ccatcggcac accagtaaga acgtcagcca acgctaactc ttcgttagtc
     6001 agcccggaat cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc
     6061 tctaacagcg gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactgcgc
     6121 gcagtggtgg cgacagtgaa atcgatatcg tgtaacgatt cagccagcgt cgggaaaact
     6181 ttaatattat caataatatc accagatcca tgtgcgaccc agcgggtggc tggctccagg
     6241 tgcgcttgac tatcgacaat ccgcagttcg ctaaaaccca tcgttttcat tgcccgcgcc
     6301 gctgccccaa tattttctgc tctggcgggt gcgaccagaa taatcgttat acgcatattg
     6361 ccactcttct tgaccaaata accgcgaaca gggtgatcac tgtcaactta ttacgcggtg
     6421 cgaatttaca aattattaac gtaagtcgca gaaaaagccc tttacttagc ttaaaaaagg
     6481 ctaaactatt tcctgactgt actaacggtt gaattgttaa aaaatgctac atatccttct
     6541 gtttacttag gataatttta taaaaaataa atctcgacaa ttggattcac cacgtttatt
     6601 agttgtatga tgcaactagt tggattatta aaataatgtg acgaaagcta gcatttagat
     6661 acgatgattt catcaaactg ttaacgtgct acaattgaac ttgatatatg tcaacgaagc
     6721 gtagttttat tgggtgtccg gcccctctta gcctgttatg ttgctgttaa aatggttagg
     6781 atgacagccg tttttgacac tgtcgggtcc tgagggaaag tacccacgac caagctaatg
     6841 atgttgttga cgttgatgga aagtgcatca agaacgcaat tacgtacttt agtcatgtta
     6901 cgccgatcat gttaatttgc agcatgcatc aggcaggtca gggacttttg tacttcctgt
     6961 ttcgatttag ttggcaattt aggtagcaaa catgcagacc ccgcacattc ttatcgttga
     7021 agacgagttg gtaacacgca acacgttgaa aagtattttc gaagcggaag gctatgatgt
     7081 tttcgaagcg acagatggcg cggaaatgca tcagatcctc tctgaatatg acatcaacct
     7141 ggtgatcatg gatatcaatc tgccgggtaa gaacggtctt ctgttagcgc gtgaactgcg
     7201 cgagcaggcg aatgttgcgt tgatgttcct gactggccgt gacaacgaag tcgataaaat
     7261 tctcggcctc gaaatcggtg cagatgacta catcaccaaa ccgttcaacc cgcgtgaact
     7321 gacgattcgt gcacgcaacc tgctgtcccg taccatgaat ctgggtactg tcagcgaaga
     7381 acgtcgtagc gttgaaagct acaagttcaa tggttgggaa ctggacatca acagccgttc
     7441 gttgatcggc cctgatggcg agcagtacaa gctgccgcgc agcgagttcc gcgccatgct
     7501 tcacttctgt gaaaacccag gcaaaattca gtctcgtgct gaactgctga agaaaatgac
     7561 cggccgtgag ctgaaaccac acgaccgtac tgtagacgtg acgatccgcc gtattcgtaa
     7621 acatttcgaa tctacgccgg atacgccgga aatcatcgcc accatccacg gtgaaggtta
     7681 tcgcttctgt ggtgatctgg aagattaatc ggctttacca ccgtcaaaaa aaacggcgct
     7741 ttttagcgcc gtttttattt ttcaacctta tttccagata cgtaattgat cgtccattgt
     7801 aacttcttta ctggctttca ttttcggcag tgaaaacgca taccagtcga tattacgagt
     7861 cacaaacatc atgccggcca gcgccaccac cagcacactg gttcccaaca acagcgcgct
     7921 atcggcagag ttgagcagtc cccacatcac accatccagc aacaacagcg cgagggtaaa
     7981 caacatgctg ttgcgccagc ctttcaatac cgcctgcaaa taaataccgt tcattaacgc
     8041 cccaatcaga ctggcgatta tccatgccac ggtaaaaccg atatgttcag aaagcgccag
     8101 caagagcaaa taaaacatca ccaatgaaag ccccaccagc aaatattgca ttgggtgtaa
     8161 acgttgcgcg gtgagcgttt caaaaacaaa gaacgccata aaagtcagtg caatcagcag
     8221 aatggcgtac ttagtcgccc ggtccgttat ttggtattga tcggctggcg tcgtcactgc
     8281 gacgctaaac gcggggaagt tttcccagcc ggtatcattg cctgaagcaa aacgctcacc
     8341 gagattatta gcaaaccagc tgctttgcca ttgcgcctga aaacctgact cgctaacttc
     8401 ccgtttggct ggcaaaaaat cacctaaaaa actgggatgc ggccagttgc tggttaaggt
     8461 catttcgcta ttacgcccgg caggcaccac agaaagttcg ccggtaccgc ttaaattcag
     8521 ggccatattc agcttcaggt tctgcttccg ccagtcccct tcgggtaaag ggatatgcac
     8581 gccctgcccg ccttgctcta acccggtgcc gggttcaatg gtcagcgccg ttccgttaac
     8641 ttcaggcgct ttcaccacac caataccacg cgcatccccg acgctaatca caataaatgg
     8701 cttgccaaag gtgatatttg gcgcgtcgag ttcgctaaga cgcgaaacat cgaaatcggc
     8761 ttttagcgtt aaatcactgt gccagacctg accggtataa atccctatct tgcgttcttc
     8821 cacgttctga ttgccatcaa ccatcaatga ctcaggtaac caaaaatgga taaaacttcg
     8881 tttccgctcc acggttttat cctcttcctg taccgtataa agctcggtca caggaatagc
     8941 gataagcggc ccaacgagtt tttgcggccc gctggtactt tggcgaatcg catcttccac
     9001 atcgctacgg taatcggcac gttcgacaat cacctgccga atcagcatta tcggaatcaa
     9061 caacagcaat actgcaccaa acagggtggt cattttccag aacagggggg atttcaacat
     9121 agtcttctcc tttgcaatgg cagcaggata cgaaggctat gtgggaagaa tttgaagcta
     9181 tgtgaagtga cggtgaagtc gaagcgaggc cagcacgcca ccttcctgca cgttgcgcag
     9241 cgtgacttcg ccgttaaaca aacgggcgac ctcactgacg aacgccaacc ccagaccgct
     9301 gcttttttgc ccatttgcac gcggcaaaga gtaaaagcgt tcaaaaatac gtgacagcgc
     9361 gtagtcagga ataccactac cggtatccag caccttaagc gtgacgtgtt cctgatccac
     9421 ttcggcgctt agcgttatac gaccgctctc gggggtaaaa tcgatggcgt tatccagtaa
     9481 attccccagc gcctgatcca gtaacgccgg ttcagcagca acattaacct cggtgggcgt
     9541 aacatgtaga gtgatgtttt tttctgccaa ctgcacggtg cgcgcttcgc tgacgcggcg
     9601 gaataatgcc gccacatcaa cagcagtcaa aacgacttcc tgacgattct ccagtcttgc
     9661 ctggcgtagt aacgtttcca ccagtgcctg cattcgcgca ttttgcgtca gaatgttgtc
     9721 agtaaaacga gccactactt caggcggcgg accttcgcgt aaaatttccg ccgcgccacg
     9781 aatcgccgcc agtgggcttt ttagctcatg ggttaacgca taaacatact gctcaatata
     9841 gtttttccct tccagcttca cgcgcatact ttccagcgcc tgcgcgagtt tacgcagctc
     9901 gctactaccg agatcgggaa gaggaacggg cttattgtca gtgacggaat cagcatagcg
     9961 agtgagccgg gcaatggagc ggttgatcca ccaaaccatg cctgcgccaa tcaccagtgc
    10021 gatccccaac aaaatggcgc tggcccataa aattcgccgc tcgctgcgtt taatgaccgg
    10081 agccatcgcc gcgttcggtt tgcctacgct caaaacgcca ataagccgcg agccgtccat
    10141 aatcggcgcg gcaacataca tcaccgaact ttcgggatcg gcagaatttt gcaacgtgct
    10201 gcgcgcacca tactgaccac gcaacgttag ccagacgtca ttccagcgcg aataatcctg
    10261 cccaacggct ttatttgccg aatcgaacaa tactttaccc tgcgaatcgg tcatatagac
    10321 atgatattca ttgcgcactt tgttaatgcc accgatattg gcgcgaaacg ggcgatgttg
    10381 tagctgatta aacgcctgcg ccagttgccc atgcgttggg tccccagaga gcaaatcggg
    10441 acgcgccagc tccgccagca acgttgcggt gtcgatcaac gtcccctccg ttgctcttcg
    10501 cacgcccggt ttaacttctt tgacaaaaat agccagtacg aaccaggccg ccaccgccac
    10561 cagtaaaaaa tagcccagca gcaaccgcat gccgatacgc attacaggcc cctcaggcta
    10621 tatcccatgc cgcgatgagt attaatcggt gaaagatcgg ggttgatggc gcgcagcttg
    10681 gcacgcaacg ttttaatgtg ggtatcaacg gtgcgatcgt aggtgtcctg cgcatcttcc
    10741 cacacgctat ccatcagttg ctggcgggac cagacgcggc ctggcgactt gagtaacgtc
    10801 ttcagcaata aaaactcata ccgcgtcagc gttaatggcg tgtcaaacca gctgatctgc
    10861 gccgcgggtt cattcaattc aaaatgacca atacggatga cgggagacgg cgacgagaac
    10921 ttcttcaccc gacgcagtaa ggtgcgcacc ctggcgcaca cttcgcgggg tgaaaacggt
    10981 ttagccacat agtcgtcagc gccaatttcc agcccaagca ggcgatcgac ctcttcactt
    11041 cgggccgtca ggaacagtac aggtaacgcc ggatggagcg ccagtaactg gcggcacaat
    11101 tcaaagccac tgatatcagg caaaccaacg tcaagaatca tgacgtcggg aacctgctgg
    11161 cgagctttat ccagcaccgg caaacctcgc tcaaagacct cgacggcaaa accttcctgc
    11221 tgcaacatgt agaccagcgt gtcggctatc ccttgctcat cttccactaa ccagaccgtt
    11281 tcccgttgca taaatcgcct ctgttattgc cgccacggca tgacaggaac cgcgctaatc
    11341 gcgtttttcg gcgaaccttc tacaactttg tcggagtaag ccagataagc gagcgcgttg
    11401 cgtttggcgt cataaaagcg cacgacctgt aacgacttaa agaccaggga cgtgcgtttt
    11461 ttgaatacta cctcgccctg agctttgccg tttttaatac gatccgacag ttcaatcggc
    11521 ccgacttgct gacaagaaat ggccgcatcg gaggtatctt ctgccaggcc caatccccct
    11581 ttaataccac cggttttcgc ccggctcaca taacaggtga catttttcac atcgggatcg
    11641 tcaaaggctt ccacaacaat tttgtgatcc gggccgatca ttttaaatac ggtgtcgacc
    11701 gaaccaatct cttctgcatg agccaatggc cccagcatta tcaggcttaa agacaggatc
    11761 aaatgcttgt atttcatatt gttaccattt ttatctatta cacgagcaat tagttcgtca
    11821 cggtaaataa catattgatt aagaacaaaa tctcaatact tttatttccg tcaagcccta
    11881 aaacatactc tactaagaaa aaaacactga atgctaaaac agcaaaaaat gctattatcc
    11941 aattacctga tgtcaggtgc tcgttgttga aaggatgagg atattttatg gatcaggccg
    12001 gcattattcg cgacctttta atctggctgg aaggtcatct ggatcagccc ctgtcgctcg
    12061 acaatgtagc ggcgaaagca ggttattcca agtggcactt acagagaatg tttaaagatg
    12121 tcactggcca tgctattggc gcgtatattc gtgctcgtcg tttgtcgaaa tcggcggtcg
    12181 cactacgcct gactgcgcgt ccgattctgg acatcgcgct gcaataccgc ttcgactctc
    12241 aacagacatt tacccgcgca ttcaagaagc agtttgccca gactcctgca ctttaccgcc
    12301 gttctcctga atggagcgcc tttggtattc gcccgccgct acgtctgggt gaattcacaa
    12361 tgccagagca caaatttgtc accctggaag atacgccgct gattggtgtt acccagagct
    12421 actcctgttc gctggagcaa atctctgatt tccgccatga aatgcgttat cagttctggc
    12481 acgattttct cggcaacgcg ccgaccattc cgccagtgct ctacggcctg aatgaaacgc
    12541 gtccgagtca ggataaagac gacgaacaag aggtattcta taccactgcg ttagcccagg
    12601 atcaggcaga tggctatgta ctgacggggc atccggtgat gctgcagggc ggcgaatatg
    12661 tgatgtttac ctatgaaggt ctgggaaccg gcgtgcagga gtttatcctg acagtatacg
    12721 gaacgtgcat gccaatgctc aatctgacgc gccgtaaagg tcaggatatt gagcgatact
    12781 acccggcaga agatgccaaa gcgggagatc gcccaattaa tctgcgctgt gaactgctga
    12841 ttccgatccg tcgttaacgc tgcaactcat ctaatgcagg ggcgtccaga tgcgagatgt
    12901 cccctgcggt ttcaacaacc cagccagacg ccagccacag gctttcctga taatccacgc
    12961 gcgaaataga acagttacgc agacgtaagc ggcgctctgc ccatgctggt aatccgagaa
    13021 tcgtactcac cagacatccc agtgcaatac catggctcac cagcagcggt cggcttccct
    13081 gcggtaagtc ccggcaagat tccagtgccg cattgacgcg atcgctgagc tcctgcattg
    13141 actcgccttc aggaatacgc ccgtcaacgg tgccattgac cagctgccga cgccagttct
    13201 cttcttcttc ggtcagagaa tcgatatgtc ttttttccag cacacccatg tttaattcac
    13261 gcaggcgaga atcaaagatg atgtcacagc cgcaggcctg ggcgatgatt tccgccgtac
    13321 gccgggtgcg tcctaaatcg ctactgataa tatgcgtaat gccaagctct ttggcacggg
    13381 ttgccacctg catcgcctgt tgctcacctt tggcggtcag cgggctgtca gactggccct
    13441 gaatacgtcg ctcggcgttc cactgcgttt caccgtggcg gactaggtat acctgtaaca
    13501 tgcttttttt ccgtatactg ctgtaacgtt aattttctga gtaatgctga ttatgcacca
    13561 agttgtctgt gcgaccacca atcccgctaa aattcaggcc attctgcagg catttcacga
    13621 gatcttcggc gaaggatcct gccatattgc atccgttgcc gtcgagagcg gtgtaccgga
    13681 acagcccttt ggcagtgagg aaacgcgcgc tggcgcacga aatcgggtag caaatgcccg
    13741 ccgtttactt ccagaggctg atttttgggt ggcgattgaa gctggcatcg atggtgacag
    13801 cactttcagt tgggtggtga ttgaaaacac cagccagcgc ggcgaggcgc gttcggctac
    13861 tttgccgtta cctgcggtga ttctgcagaa agtgcgtgaa ggtgaggcgc ttggtcccgt
    13921 gatgtcgcgt tataccggta ttgatgagat tggtcgtaag gaaggggcga tcggcgtgtt
    13981 taccgccggg aaactcactc gcgccagcgt ctatcaccag gcggtgatcc tggcgctgag
    14041 tccgtttcac aatgccgtgt atcaggcatt gtaggacgga taaggcgttc acgccgcatc
    14101 cggcattcgg tgcacgatgc ctgatgcgac gctgccgcgt cttatcaggc ctacaaaatc
    14161 aatcgctttt cagcaacacc tcttccagcc actggcgcag ctcaacgggc gcggctttca
    14221 ggctgttaga tccacgcgta atcgtcgcga tgcccgcgcc gagttcattt tttaactcac
    14281 gctggctcat ttcgccgcgc aacagctctt cgacaatacg cacgcgagtc cccaacgctt
    14341 cgcgctcatc tggcgtcagc atcaggttta acaacggtaa atggagatcg ttttggtagg
    14401 cattcttaag caggtcgaca aaacgtaacc actcctggtg acgctgttct gccatcgctg
    14461 ctgaataggg tgattgttgg gccataatat gtcgccattg ttagcggggg aagcaaaatg
    14521 cctcccccgg ttgtactcgc taaagagtac gatagcatat cataaacgtg cggatcagta
    14581 acgacgtccc cattccgtgg cgctcatcaa cgtcggttta tcccccatga aatagcggta
    14641 gtaagcgtca taagccagca cgttcttcac ataaccgcgc gtctcggaga acggaatact
    14701 ctcgacaaat gccacagcgt cgatacgccc ggcgctgttg ccaagccagg ttcgcacccg
    14761 tccaggtccg gcgttataag ctgctgagga gaaaatacgg ttattgccaa actgctgata
    14821 aacatattgc aggtaactgg tgccaatgtt gatattcgtt tccggatcca gcaattgccc
    14881 agggctgcta taaccaggaa tagagaacat cttcaccgta tgggtcgctg taccaggcat
    14941 aatctgcatc aggccgctgg cccctaccgg tgatttcact ttcggattcc aggcgctctc
    15001 ctgacgggca atcgccatcg catagctttg cgggatctct ttcccactgg tgtaacgttt
    15061 gaaaagatcg ttgtaagcca gcgggaatcg ctcttccaga tgatcccaaa gcttcccggc
    15121 gatcgttgcc tgaacgctaa gatcccacca ttggttattg aaagcatacc gagccagttg
    15181 agcctgctct gtttttgact tgctcttcac cagattggcc cactcgctac gcgcggtgtt
    15241 atcgagattc cagtacatca actcgcgcac gcgcgccatc tccggcccct gagtcagggc
    15301 gctgtcaaca ttctgcggcg ctttatcaat cttcagctca tactcttctc cgatgcgttg
    15361 tgctgcaacc atcgggtaga aaccacgctg ttgcatgagt tgatgcaaaa tctctttcgc
    15421 ttcagcttca cgtccgcgtt ccagcaataa atccgcctgc cagtaacgcc attcatcttt
    15481 ctcttttgct tccatcggca gacgcgccag ccaggtattc aggccgtgac gatcgccggt
    15541 gccaagtgcc attcgcacac ggcgttcaat caacgacgtg gattgcgagc gcataatggc
    15601 gtcatcgcgc catttcgcct gctcgtcggt gacatcgttg cccatcaaac gccaggcgac
    15661 gatatcgcgc agctcctgaa tctgatcttc attaagctgc tgcgcctggg caagcgacag
    15721 gatcatcagc cgtgcatttt cagcatcctg ccgcgccaca ctggcaaacg ccaccgctgc
    15781 catttgacgg gtaaaatcgg tcgctccggt tgtacgcgcg aaggtcagta ccgtattagg
    15841 gttgttcgcc agtgaaatga ttgccgaggc gatagtctgg taatcggcag gcatctgccc
    15901 tgccagcact gttaccaggc cagtgttacc cgctttcatc gccagacgga tacgctctaa
    15961 atacgccagc ggatcttgtt tacctgacgc acgccagacg ctaaataact tgtcacaggc
    16021 gttaggctgg ctcttgccgg ttagccacag ctctttcgcc ccttgccagg cttcttcact
    16081 ctgcccggtg ttccatttcg catagtagta attacattgc gcttcggtag ttccgggctt
    16141 ttccgggcta aaggctaaca agccacgcca gtcttcacgc cgcgccagtt cattgacgaa
    16201 acgagattgc agcgtgcgag cgggaggaag cgtggggtta gcgcgaacaa agttagtgac
    16261 cgtcaccgcc ggttgattca tcagatcatc ggtgatctgg cggtattcca ggtagggata
    16321 aagcggataa tccttcagtc caggcatcat ttgttcgacc acatccattt gtcgattatc
    16381 ccaggcctgc ttgatttgcg cgtaacggct acgctgctca tccagtgagt cggctcgcgc
    16441 cacgctgctg accgtcagca gacagacacc ggcagccaac agccgccagg taacttgttt
    16501 ggctttttcc acaagcgcat cctctaagtg taaatacatc aatgcagcgt catgccgcgt
    16561 aatagattta tgctaaccag tcattgccgt ttacgccacg ttacggacac ttttttactt
    16621 ttactgcgaa ggtgatcgac caactgggat taggcgggga aaaaggctac acttcgcctt
    16681 tgaaaactca tcatcacgat aaacatagag gcgaagtcca acgtggctca attcgtttat
    16741 accatgcatc gtgtcggcaa agttgttccg ccgaaacgtc atattttgaa aaacatctct
    16801 ctgagtttct tccctggggc aaaaattggt gtcctgggtc tgaacggcgc gggtaagtct
    16861 accctgctgc gcattatggc gggcattgat aaagacatcg aaggtgaagc gcgtccgcag
    16921 ccagacatca agattggtta cctgccgcag gaaccgcagc tgaacccgga acacaccgtg
    16981 cgtgagtcca ttgaagaagc ggtttctgaa gtggttaacg ccctgaaacg cctggatgaa
    17041 gtgtatgcgc tgtacgccga tccggatgcc gattttgaca agctggccgc tgaacaaggc
    17101 cgtctggaag agatcattca ggctcacgac ggtcataacc tgaacgtaca gctggagcgt
    17161 gcggcggatg cgctgcgtct gccagattgg gacgcgaaaa tcgctaacct ctccggtggt
    17221 gagcgtcgtc gcgtggcgtt gtgccgcctg cttctggaaa aaccagacat gctgctgctc
    17281 gacgaaccga ccaaccacct ggatgccgaa tccgtggcct ggctggaacg cttcctgcac
    17341 gacttcgaag gcaccgttgt ggcgattact cacgaccgtt acttcctcga taacgttgca
    17401 ggctggatcc tcgaacttga ccgcggtgaa ggtattccgt gggaaggcaa ctactcctcc
    17461 tggctggagc agaaagatca gcgcctggcg caggaagctt cacaagaagc ggcgcgtcgt
    17521 aagtcgatcg agaaagagct ggagtgggta cgtcagggaa ctaaaggccg ccagtcgaaa
    17581 ggtaaagcac gtctggcacg ctttgaagag ctgaacagca ccgaatatca gaaacgtaac
    17641 gaaaccaacg aactgtttat tccacctgga ccgcgtctgg gcgataaagt gctggaagtc
    17701 agcaacctgc gtaaatccta cggtgatcgc ctgctgattg atgacctgag cttctcgatc
    17761 ccgaaagggg caatcgtcgg gatcatcggt ccgaacggcg cgggtaaatc gaccctgttc
    17821 cgtatgatct ctggtcagga acagccggac agcggcacca tcactttagg tgaaacggtg
    17881 aaactggcat cggttgatca gttccgtgac tcaatggata acagcaaaac cgtttgggaa
    17941 gaagtttccg gcgggctgga tattatgaag atcggcaaca ccgagatgcc aagccgcgcc
    18001 tacgttggcc gctttaactt taaaggggtt gatcagggta aacgcgttgg tgaactttcc
    18061 ggtggtgagc gcggtcgtct gcatctggcg aagctgctgc aggttggcgg caacatgctg
    18121 ctgctcgacg aaccaaccaa cgacctggat atcgaaaccc tgcgcgcgct ggaaaacgcc
    18181 ctgctggagt tcccgggctg tgcgatggtt atctcgcacg accgttggtt cctcgaccgt
    18241 atcgccacgc acatcctgga ctaccaggat gaaggtaaag ttgagttctt cgaaggtaac
    18301 tttactgagt acgaagagta caagaaacgc acgctgggcg cagacgcact ggagccgaag
    18361 cgtatcaagt acaagcgtat tgcgaagtaa tgcgtaaaat gccggatgcg gcgtgaacgc
    18421 cttatccggc ctacaaacca agaaatttca atacgttgtg aatctccagg cctgataaga
    18481 cgcggtaagc gtcgcatcag gcattgagca ccgaatgccg gatacggcgt gaacgccttt
    18541 tccggcctac aacagcctta gtatccgcaa attcgtagcg ggtcacccgt gctgattctt
    18601 ttaaaccttg tttaatctcg acagcctctt ccagagaggt tttcaactca tcaaaaaact
    18661 tattttatac ctcgcctttg agccgtttca tcgcggttat ctctgctccc ccatcatctc
    18721 ccgcaccagc tccacgcagc gcaggaaacg gctgtcataa tcgtcctctt caacccgcac
    18781 gaattcgata ttgttctctt ccagcatctc caccagcaag ttctggaact ctttgcgatc
    18841 caccgaactg ccgaggctgc gtaaaccatc cgccacccac ggcgtgttgt tctccagcag
    18901 gatcaccaga tcgaaacggt attcatcaat caacgcctgt acgaacggat gctcacgccc
    18961 ttcgtacttt ttgcagaacg cctgggtagt gacaaaatca gtgtcaataa acgccacttt
    19021 attggcatat ttcactgcaa aatcaatgta ttgtgcgtgg cccagcgcga ttttatcgta
    19081 atcggaatac tgcaatgcga tctcatcacc gccgaggtgt gaaaagacat aatcgcgacc
    19141 atattcccac gcactggtgg tattgaagat attggcaagt ttgtttacca gggtggattt
    19201 accgctcgac tcgccaccaa ggatcgccac ggtacgtaca aagaacggct tcacttccgt
    19261 aggaatatat tcccagtagc ggaacgggtt ttcgcggatc tgcgcaccgc tgatactcat
    19321 aaaggtacgt ttcggatcga ccagcaccgt atcgatcccc agatgttcca tatactgtgg
    19381 cgcatcggct tcttccgagg tgtagatcag gtccggctga atcccctttt cagccataaa
    19441 ctttttgatg ccgttgctcc acacatccca gccgtgcgga tacggctcca tgccctcttc
    19501 gttgaaagca tgaatgcgaa tatttttctg atatttaaaa gtttgtaata accagcgcag
    19561 acgatccggc accgtgggct gctgcgacat ggcgctgtct tcaaacaacg cgcggtcgcg
    19621 ggtatcgtca aaacccataa tgatatgcag ctcgtcaacc tggctacagg cgcgctggat
    19681 aaggtagata tgtccggtat gcagcgggta gaacttaccg aatacgacac caatggtttt
    19741 cttctgtcgg ggaaattcaa gccccaaaaa acggtgcaac gcctccagct tttgcgcgct
    19801 ggggctttta attttggcat tcagtaactg gcttaaatac cctttggtca taccgctggc
    19861 atcagctacc tgctgtagcg tgcagccctg ttgcttgatg gcagttttca ggtaatcaaa
    19921 tgacgacata tctccctccg tatctctcat tataagtcgt cgaacacgct aagcgcgtcg
    19981 gagagttttt taacgccaaa aatctgcatc ccttccggcg cttttttcgg tacgttagcc
    20041 gccggaacaa tcgcccggcg aaaaccgtgt ttcgccgctt ctgaaattcg ttcctgaccg
    20101 ctgggcaccg ggcggatctc ccctgccagc ccgacttcac caaacaccac cagatcctgt
    20161 ggcagcggtc tgtcacgcag gctggaaacc atcgccagca gtaacgctaa atcggcactg
    20221 gtttcggtta ccttcacgcc gccgaccacg ttcacaaata catcctgatc ggccatttgc
    20281 agaccaccgt gacggtgcaa gacagccagc aggattgcca gacggttttg ttccagcccc
    20341 actgccacgc ggcgcggatt cgccatcatc gagtgatcga ccagcgcctg aatctccacc
    20401 agcaacggac gcgttccttc ccacaccacc atcacggagc tgccggaggt cacttcatct
    20461 ccgcgactta agaaaattgc cgaagggttg ctgacttcac gcagcccctg ttcggtcatc
    20521 gcgaagacgc ccagctcatt caccgcgccg aagcggtttt tatggctgcg caaggtgcga
    20581 aaacgggagt cggcatcacc atccagaagc accgaacagt cgatacagtg ttccagcact
    20641 ttagggccag ccagcgagcc atctttggtt acgtgcccaa ccatgacaat cgccacaccg
    20701 cgcgttttgg cgaagcgcgt cagataagcc gccgtttcac gcacctgcgc cacgctacca
    20761 ggcgatgatt gtacatccgc catatgcatc acctggatcg agtcaattac catcagtttc
    20821 ggttgctctt cttcggcaat caggcagatc tgttcgatgc tggtttccga caacatattg
    20881 agattgtcag tcggcaggcc aaggcgatga gcgcgcattg ccacctgttg cagcgactct
    20941 tcgccagtga catacagcgt tttcatctgc tgggccagtt tgcacagcgt ttgcagtagc
    21001 agcgtggatt tccccgcacc tgggttaccg ccaatcagaa tggcgcttcc tggcaccacg
    21061 ccgccgccta gtacgcggtc gaactcttta aatccggtgg aaaaacgcgg cagctcttca
    21121 aggctgatat cggagagttt ctggactttt gccaccccgg cgctaccggc atagccgctg
    21181 agacgctcgt tacgcgccac cgttggcgac gcagcaagac gcacctcggt gatggtgttc
    21241 caggcatgac aggcactgca ctgcccctgc cagcgcggat aatcggcccc gcattcatta
    21301 caaacaaagg cgcgttttgg agcttttgcc acggtgtacc tcgttaatgc tgtgccgccc
    21361 gcaggatggc gggcgagcaa ttacttctga ttcaggctgc cagagaggat gcagaatacc
    21421 cccatcaggt cagcgtgacg gatggtgact tccgcctttt cattcacttt tggcttggca
    21481 tggtaggcaa tccccagccc tgctgctttg atcatcggca ggtcattagc tccatcgcca
    21541 atcgccacgg tctgcgccag cgggatttca tactcctgcg cgaggcgagt caaagttttc
    21601 gctttgtact gcgcgtctac gatgtcgccg atcacattgc cggtaaattt accgtccatg
    21661 atctccagtt cattggctac cacggcggtc aggcgcagct tgtcgcgcag atattcagca
    21721 aagaaagtaa agccgccgga ggcaatcgcc actttccagc ccagcgtttc cagcttgagc
    21781 accagttgcg ttaagcctgg catcagcggc agattttcac gcacctgttg cagaatattg
    21841 gcgtcagcgc ctttcagcgt cgccacacgg ctgcgcaggc tggcggtaaa atcgagttcg
    21901 ccgcgcatcg cccgttcggt tacttccgcc accatctcgc ccgttccggc cagtttggca
    21961 atttcatcaa tacattcaat ctggatggcg gtggaatcca tgtccatcac cagcaaaccc
    22021 ggcgtgcgca ggtgcgggat tttccccagc ggggcgacat ccagttgcgc ttcatgggcc
    22081 aggcgcgtag cccgtgcggt gagtgaacct gccagacgaa tcacctgata atcttccacg
    22141 caccaggcgg caacaatcac catcgccgca cccagtttgc tctggtattg ggtcagacgc
    22201 tgtttatcca gcccacgacc atacagcagc cagccgctac gacctgcgtg gtaatccagt
    22261 ggcatcactt catcaccact taatgaaaga ggcagaccag gccataaaga gacatcttca
    22321 ggcaggtcgc accaggtaat gttaggcatt aaggctcctg taaaatcgtt cgaagcaggg
    22381 aaaataacgc atgaggctac cttgtatcca ttgcttctgg caacattaag tctcaaattt
    22441 tcaaagggtg gaagatggct cgcacaaaac tgaaattccg gctgcatcgg gcagtgattg
    22501 tcctgttctg tcttgccttg ttagtggcgc tgatgcaggg agcgtcatgg tttagtcaaa
    22561 accaccagcg acaacgtaat ccacagctgg aagaactggc ccgcaccctg gcgcgtcagg
    22621 taacgctgaa cgttgcaccg ctgatgcgta ccgactcacc ggatgaaaaa cgcattcagg
    22681 cgatcctcga tcagttaacg gatgaaagcc gtatcctcga cgcgggtgtg tatgacgaac
    22741 aaggcgatct tatcgcacgt tctggcgaaa gcgtcgaagt gcgcgaccgg ctggcgctcg
    22801 acggtaaaaa agcaggcggc tattttaacc agcagattgt cgagccaatt gcgggtaaaa
    22861 acggaccgct cggctatctg cgcctgacac tcgacaccca tacgctcgcc accgaagccc
    22921 aacaggtgga taacaccact aacattttac gcctgatgtt gctgctctca ctggcaatcg
    22981 gcgtagtgct gacccgcacg ctgctacagg gtaaacgcac ccgctggcag caatcgccct
    23041 tcctgttaac cgccagcaaa ccggtgccgg aagaggaaga aagcgagaaa aaagagtgac
    23101 ccattactac aagaaaggaa atcgttatgt ccacattacg cctgctcatt tctgactctt
    23161 acgatccgtg gtttaacctg gcggtggagg aatgtatttt tcgccagatg cccgccacgc
    23221 agcgcgttct gtttctctgg cgcaatgccg acacggtagt gattggtcgc gcgcagaacc
    23281 cgtggaaaga gtgtaatacc cggcggatgg aagaagataa cgtccgcctg gcgcggcgca
    23341 gtagcggtgg cggcgcggtg ttccacgatc tcggcaatac ctgttttacc tttatggctg
    23401 gcaagccgga gtacgataaa accatctcta cgtcgattgt gctcaatgcg ctgaacgcgc
    23461 ttggcgtcag cgccgaagcg tccggacgta acgatctggt ggtgaaaacc gccgaaggcg
    23521 accgcaaagt ctcaggctcg gcctatcgcg aaaccaaaga tcgtggcttc caccacggca
    23581 ccttgctgct caatgccgac cttagccgcc tggcaaacta tctcaatccg gataaaaaga
    23641 aactggcggc gaaaggcatt acctcagtgc gttcccgcgt gaccaacctc accgagctgc
    23701 tgccggggat cacccatgag caggtttgcg aggccataac cgaggccttt ttcgcccatt
    23761 atggcgagcg cgtggaagcg gaaatcatct ccccggacaa aacgccagac ttgccaaact
    23821 tcgccgaaac ctttgcccgc cagagtagct gggaatggaa cttcggtcag gctccggcat
    23881 tctcgcatct gctggatgaa cgctttacct ggggcggcgt ggaactgcat ttcgacgttg
    23941 aaaaaggcca tatcacccgc gcccaggtgt ttaccgacag cctcaacccc gcgccgctgg
    24001 aagccctcgc cggacgactg caaggctgcc tgtaccgcgc agatatgctg caacagaagt
    24061 gcgaagcgct gttggttgac ttcccggaac aggaaaaaga gctacgggag ttatcgacgt
    24121 ggatagcggg ggcggtgcgg taactcgctg attaaaatat ccagggaagg aaatatgatc
    24181 gatctggaaa accaggaacg ggagattatc aatttaatgt tcagccagcg tatttcctgg
    24241 ctagccgccg tgcgtatcag gcataaatta tcgcttgctg aagtaagtaa aatgttaggt
    24301 atctcgatta attctctaaa atagatcgaa aagacggaac ggtttagctc aaacatcaaa
    24361 agcaaaatgg cagaaattta tggttgtcca ccagagttgc tgatatgccc ttcctggatg
    24421 acggctgaac ataaatgaat agattcgtga atagatactt gaatagattt tattttctat
    24481 ccatatgaaa aataatagta aatctataaa tcagttgcta ttgatgaata gatactaaaa
    24541 agccggagca gtctcccact ccggcttcac acgacaatcg cctttcagcg aaacacaatt
    24601 actctttatc gcccagcaga acggattcca gtgcgatttt gatcatgtcg ttgaaggtgg
    24661 tctgacgctc agcggcagtg gtctgctcgt gagtgcggat gtggtcagac acggtgcaga
    24721 tggtcagggc tttcgcgcca aattctgcag cgacgccgta gataccagca gcttccattt
    24781 ccacgccgag gatgccgtat ttttccatca cgtcgaacat ttcgccgtcc ggagagtaga
    24841 acaggtcagc ggagaacagg ttaccaacgc gagcatcaac gcccagtgct ttagccgcgt
    24901 ctaccgcgtt acgcaccatg tcaaagtcag cgatagcggc aaagtcatgg tctttaaaac
    24961 ggatacggtt aactttggaa tcggtgcagg cacccatacc gataacgacg tcgcgcagtt
    25021 ttacgtgcgg cagaactgcg ccacaggaac ccacgcggat aattttcttc acgccgaaat
    25081 cggtgatcag ttctttggtg tagatggagc aggacgggat acccataccg tgacccatta
    25141 cggaaatttt gcggcctttg taagtaccgg tgaagcccag cataccgcga acgttgttca
    25201 cttcacgggc atcttcaagg aaagtttcag caatatactt cgcacgcagc gggtcgcctg
    25261 gcatcaaaac tacgtcagcg aaatcgccca tttctgcatt aatgtgtggg gtagccattg
    25321 ttttatcctt atcaacatgt tttcaccctc tccctgtggg agagggccgg ggtgagggca
    25381 tcaagccgca cccattatca gaacatggct ttgccatatt ccatatcaga agtaccaaaa
    25441 tattttgcca gagtctggcc gatatccgcg aaggtttcac ggtgacccag tgaacccggt
    25501 tttactttcg ggccgtaaac cagtaccgga atgtgttcac gcgtgtggtc agtaccggtc
    25561 caggtcggat cacaaccgtg gtcagcggtg aggatcagga tgtcgtcatc gcgcagcaga
    25621 gacatcagct ccggcagacg acggtcgaac agctccagac ccgcagcata accggcaacg
    25681 tcgcgacggt ggccccagga agagtcgaag tcaacgaagt tggtgaagac gatggtgtta
    25741 tcacccgctt ctttcatctc tttgatggtg gcgtcaaaca gcgcgtccag gccggtcgct
    25801 ttcacttttt tggtgatacc gcagttggcg tagatgtcgg caattttacc gaccgaaacc
    25861 acctggccgt gtttttcatc aaccagtttc tgcagcacgg tcggtgctgg cggttcaaca
    25921 gccaggtcgt gacggttacc ggtacgctgg aagttaccgg ctttgtcacc gataaacgga
    25981 cgagcgataa cacgaccgat gttgtagccg ccgttggtca gctcttcacg ggcgatttcg
    26041 cacagttcgt agagtttatc cagaccgaaa gtttcttcat ggcaggcaat ctggaacacg
    26101 gagtcagcgg aggtatagaa aatcggcttg ccggttttca tgtgctcttc gcccagttga
    26161 tccagaatga ccgtaccgga agagtggcag ttaccgaggt aacccggcag attagcgcgt
    26221 tcgaccagtt tatccagcag ctcttgcggg aagctgtttt cgtgatcgga gaaatatccc
    26281 cactcaaaca gaaccgggac accggcgatt tcccagtgac cagacggggt atctttaccg
    26341 gatgacattt cgtgcgccca tgcgtacgcg ccgataactt cagcgttgcc gtccattccc
    26401 gccggaatga aaccggtaga accttcgtgt gctttcgcca gccccagacg ggtcagattt
    26461 ggcagattga gcgggccttt acgaccgtta tcagcttcgc ctttggcaca agcttctgcg
    26521 atatgaccca gggtgtcagc cccgacgtca ccaaagcgtt ctgcatcttc tgtagcgccg
    26581 atgccgaatg agtccagcac cataataaat gcacgtttca tatgttctcc gtactcagtg
    26641 cttcaaattt aaatgatcag atcagtatac cgttattcgc tgatacggcg atagacagtt
    26701 ggtgtgcttt ccggtgcttt atcggcaagt ttaattgccg ctttcaccgc tttcgccgct
    26761 tcctgccagc tgttttcgtc tttcgcgtgg ataaccgcca gcggacgctg accgtctacc
    26821 tggtcgccca aacgcgccat atcagtaaag ccgacgctgt aatcgatggt gtcagatgcc
    26881 tgacggcgtc cgccgcccat tgcaaccact gccatcccca gcgcgcgggt atccatttca
    26941 ctgacaaaac cttcggtatc agcatagact gctttcgtca gcatcgctgt cggcagatac
    27001 ttcgcgtagt tctcaacgaa gtcggtcggg cctttttgtg ccgctaccat acgaccaaag
    27061 acttctgccg ctttaccgtt gtccagcacc gcctgcaatt tcgcgcgcgc ttcggcgtca
    27121 tctttcgcca gtttgccgga gatcagcatc tccacgcaca gtgccatcgt gacatcaaac
    27181 agacgcgggt tacgatattc acccgtcagg aactgcaccg cttcacgaac ttcaaccgcg
    27241 ttacctgcac tggaggccag tacctgattc atgtcggtga gcagcgcggt ggtgcgcacg
    27301 ccagcgccgt tagccacgcc aacaatcgct tcggcaaggg cttcagagag ttcgtaggtc
    27361 ggcataaacg cgccgctacc cactttcacg tccatcacca gcgcatccag accttccgcc
    27421 agtttcttcg ccaggatcga ggcagtgatc agcgggatgg agtccacggt tgcggtaata
    27481 tcgcgggtcg cgtagaaacg tttatctgcc ggagccaatg agctggtctg gccgataatc
    27541 gccacgccga cgtctttaat aatttcgcgg aaacggttgt catccgggaa aatgtcgaag
    27601 ccagggatgg attccagttt gtcgagcgta ccgccagtat gaccgaggcc gcgaccagag
    27661 atcatcggaa tatagccgcc gcaggctgcg accatcggcc ccaacatcag cgaagtcaca
    27721 tcgccgacgc cgccggtcga gtgtttatca acaatcgggc cattcagatg caggcttttc
    27781 cagtcgagaa cggttcctga atctcgcatc gccatggtca gcgagacacg ctcaggcatt
    27841 gtcatatcgt ggaagaaaat ggtcatcgcg agggcggcaa tctgcccttc ggagatagtg
    27901 ttgtcgcgaa taccgttgat aaagaaacga atttcttcat cgctcagcgc atgaccatca
    27961 cgttttttac gaataatttc ttgtgcgaga aacaaggtaa cctcccgagg aaaagagtat
    28021 gaggcggctt ttgccggatg cgtctacgcc ttatccggcc tacgggtaga tacgtctcgt
    28081 aggcctgata agcgcagcgc atcaggcgta aagcatctta cttagtagct gctggcgctc
    28141 ttaccgtcgc cgtggcccag agccttcagc aggcttgcca gcaggctgga agcaccaaag
    28201 cggtagtggc gcgcatcggc ccagtcagcg ccgaacagtt catctgcgat ggcgagatat
    28261 ttctgcgcat cttccgcagt acgcacgccg cccgccggtt tgaaaccaac ggttttttct
    28321 acgcccatat cacgaatcac ttccatcatg atgcgcgcgc tttccggcgt cgcgttcact
    28381 gccactttac cggtagaggt tttgatgaag tcagcacccg ctttgatgga gatttcagac
    28441 gctttacgga tcagcgcttc gtctttcagt tcgccggttt cgatgatcac tttcagcagt
    28501 acattcgctg ccgcgcaagc ctctttacag gctttcacca ggtcaaaacc aacctgctcg
    28561 ttacccgcca tcagcgcacg gtacgggaac accacgtcaa cttcatcggc accgtaggcg
    28621 attgccgcac gggtttctgc cagcgcgatt tcgatgtcgt cgttaccgtg tgggaagtta
    28681 gttaccgtag caatacggat ttccggggtg ccctgctctt tcagcgtttt gcgagcaatc
    28741 gggataaagc gaggatagat acagatagcg gcggtattgc cgaccggagt tttggcctga
    28801 tgacacagag caattacttt ctcgtcggtg tcgtcgtcat tcagggtggt caggtccatc
    28861 aatttcagtg cacgcaggct gcttgctttc agatcagtca tttcattctc ctggcttgtc
    28921 gccaataaaa ttcaccttgc gagtttgtta gtattctaac atctactccg caacacactt
    28981 cgatatacat cacaattaag gaaatctact tacaagtttg catcactgta atgcgatctg
    29041 gttcaaataa ttcactttca aatgaatgcg tcagtggtgg caaacgcatc aggatcaaag
    29101 tgaacatcac gaaacttctt acaatggcgc atcaccttcg gcataaaagg aaaagaagat
    29161 gcccacttct catgaaaatg cactgcaaca acgttgccag caaattgtca ccagcccagt
    29221 gcttagcccg gagcagaagc gccattttct ggctctggaa gcagaaaaca atctgcctta
    29281 tccgcagctt cctgccgaag cccgccgcgc gctggatgag ggtgtaatct gcgatatgtt
    29341 tgaaggtcat gcgccgtaca aaccgcgcta tgtcttaccc gattacgccc gttttctggc
    29401 gaacggttcc gaatggctgg agctggaagg cgcgaaagat cttgatgacg cactctctct
    29461 gctgaccatt ctttaccacc acgtaccgtc ggtcacatcg atgccggtct acctggggca
    29521 actggatgcg ttgttgcaac cgtatgttag aattctaaca caagacgaga tcgatattcg
    29581 aataaaacgt ttctggcgtt acctcgacag aaccctgcca gacgccttta tgcacgccaa
    29641 tatcggcccg tctgattcgc ccattacccg cgcgatctta cgtgcagacg cagagttgaa
    29701 gcaggtttca ccgaacctga cctttatcta cgatcctgaa atcacccctg atgacctgct
    29761 gctggaagtg gcgaagaaca tctgtgaatg tagcaaaccg cacatcgcca acggtccggt
    29821 gcatgataaa attttcacaa aagggggcta cgggattgtg agctgttaca actcactgcc
    29881 gctggcgggt ggtggcagca cgctggtacg ccttaacctg aaagccattg ccgagcgcag
    29941 cgaatcgctg gatgacttct ttacgcgcac tctaccgcac tactgccagc agcagatcgc
    30001 catcatcgat gcgcggtgtg aattcctcta tcaacaatca cacttctttg agaatagctt
    30061 cctggtgaaa gaagggctga ttaaccctga acgttttgtg ccaatgtttg gcatgtatgg
    30121 gctggcggaa gcggttaact tgctgtgtga gaaagaaggg attgccgcac gttacggtaa
    30181 agaagccgcc gcaaatgaag tgggttatcg catcagcgcg caactggcgg agtttgtcgc
    30241 caataccccc gtgaaatatg gatggcaaaa acgcgccatg ttacacgcac agtcggggat
    30301 cagttccgat atcggcacca cgccgggcgc gcgtttgccg tatggcgatg agccagatcc
    30361 gatcacccat ctgcaaactg tcgccccgca tcatgcttat tattattccg gcatcagcga
    30421 cattctgacg ctcgacgaaa ccatcaaacg taacccgcag gcactggtac agctttgcct
    30481 cggtgccttt aaagccggaa tgcgtgaatt taccgccaat gtcagcggta acgatctggt
    30541 tcgcgttacc ggttatatgg tgcgtttgtc ggatttagaa aaatatcgcg ccgaaggttc
    30601 acgcaccaac accacctggc tgggcgaaga agccgcacgc aacactcgta ttctggaacg
    30661 ccagccgcgc gtgataagcc atgaacagca gatgcgcttt agtcagtaag attatcccct
    30721 tctcctgcgt tgacgggcca ggcagtcgtc tggctctgtt tttgcagggc tgcaatctgc
    30781 gctgcaaaaa ctgtcacaat ccgtggacga tgggacgttg caatgactgt ggggagtgcg
    30841 tgccacagtg tccgcatcag gcgttgcaga ttgttgacgg caaagtggtg tggaacgctg
    30901 tggtctgcga gcaatgtgat acctgcctga agaggtgtcc gcaacatgcc acgccaatgg
    30961 cgcaatccat gagcgtggat gaggtgctta gccatatccg caaagcggtg ctgtttattg
    31021 aagggataac ggtaagcggc ggcgaagcca cgacccaact gccgtttgta gtggcgctgt
    31081 ttactgctat caaaaacgat ccgcaactgc gccacctcac ctgcctggtg gacagtaacg
    31141 gtatgttgag cgaaaccggc tgggaaaaat tactcccggt gtgcgacggc gcaatgctcg
    31201 atctcaaagc gtgggggagc gaatgtcatc agcaattaac cggacgcgat aatcagcaga
    31261 ttaagcgcag catctgtttg ctggcagagc gcggtaagct ggcggaactg cgtttgctgg
    31321 tgatccctgg ccaggtggat tatttgcaac acatcgaaga actggcggcg tttatcaagg
    31381 gactcggcga tgttccggta cgcctgaacg cgtttcatgc ccacggcgtg tatggcgagg
    31441 cgcaaagctg ggcgagcgcc acgccggaag acgttgagcc gttggctgat gcgttaaagg
    31501 tgcgcggggt gagccggttg atatttccgg cgctctattt gtgacatgcc ggatacggcc
    31561 tacggttcgg cacagac
//
LOCUS       DAZGXZ010000051        31543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000051 DAZGXZ010000000
VERSION     DAZGXZ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.051"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..226
                     /locus_tag="ACHKJF_002419"
                     /pseudo
     CDS             137..226
                     /locus_tag="ACHKJF_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            254..997
                     /gene="yciC"
                     /locus_tag="ACHKJF_002420"
     CDS             254..997
                     /gene="yciC"
                     /locus_tag="ACHKJF_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP0949765.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            1027..1566
                     /gene="yciB"
                     /locus_tag="ACHKJF_002421"
     CDS             1027..1566
                     /gene="yciB"
                     /locus_tag="ACHKJF_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP0949766.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            1671..2069
                     /gene="yciA"
                     /locus_tag="ACHKJF_002422"
     CDS             1671..2069
                     /gene="yciA"
                     /locus_tag="ACHKJF_002422"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP0949767.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(2109..2828)
                     /gene="tonB"
                     /locus_tag="ACHKJF_002423"
     CDS             complement(2109..2828)
                     /gene="tonB"
                     /locus_tag="ACHKJF_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP0949768.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            3052..3348
                     /gene="yciI"
                     /locus_tag="ACHKJF_002424"
     CDS             3052..3348
                     /gene="yciI"
                     /locus_tag="ACHKJF_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP0949769.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            3647..4900
                     /gene="kch"
                     /locus_tag="ACHKJF_002425"
     CDS             3647..4900
                     /gene="kch"
                     /locus_tag="ACHKJF_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP0949770.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(4955..5128)
                     /gene="yciY"
                     /locus_tag="ACHKJF_002426"
     CDS             complement(4955..5128)
                     /gene="yciY"
                     /locus_tag="ACHKJF_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP0949771.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            5271..6731
                     /gene="cls"
                     /locus_tag="ACHKJF_002427"
     CDS             5271..6731
                     /gene="cls"
                     /locus_tag="ACHKJF_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP0949772.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            6766..7095
                     /gene="yciU"
                     /locus_tag="ACHKJF_002428"
     CDS             6766..7095
                     /gene="yciU"
                     /locus_tag="ACHKJF_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP0949773.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(7148..8152)
                     /gene="oppF"
                     /locus_tag="ACHKJF_002429"
     CDS             complement(7148..8152)
                     /gene="oppF"
                     /locus_tag="ACHKJF_002429"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP0949774.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGIT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(8149..9162)
                     /gene="oppD"
                     /locus_tag="ACHKJF_002430"
     CDS             complement(8149..9162)
                     /gene="oppD"
                     /locus_tag="ACHKJF_002430"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP0949775.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(9174..10082)
                     /gene="oppC"
                     /locus_tag="ACHKJF_002431"
     CDS             complement(9174..10082)
                     /gene="oppC"
                     /locus_tag="ACHKJF_002431"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP0949776.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(10097..11017)
                     /gene="oppB"
                     /locus_tag="ACHKJF_002432"
     CDS             complement(10097..11017)
                     /gene="oppB"
                     /locus_tag="ACHKJF_002432"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP0949777.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(11103..12734)
                     /gene="oppA"
                     /locus_tag="ACHKJF_002433"
     CDS             complement(11103..12734)
                     /gene="oppA"
                     /locus_tag="ACHKJF_002433"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP0949778.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            13018..13314
                     /locus_tag="ACHKJF_002434"
     CDS             13018..13314
                     /locus_tag="ACHKJF_002434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949779.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(13472..14119)
                     /gene="ychE"
                     /locus_tag="ACHKJF_002435"
     CDS             complement(13472..14119)
                     /gene="ychE"
                     /locus_tag="ACHKJF_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP0949780.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(14150..14203)
                     /gene="ychE"
                     /locus_tag="ACHKJF_002436"
     CDS             complement(14150..14203)
                     /gene="ychE"
                     /locus_tag="ACHKJF_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP0949781.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            14596..17271
                     /gene="adhE"
                     /locus_tag="ACHKJF_002437"
                     /gene_synonym="adhC"
     CDS             14596..17271
                     /gene="adhE"
                     /locus_tag="ACHKJF_002437"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP0949782.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(17573..18190)
                     /gene="tdk"
                     /locus_tag="ACHKJF_002438"
     CDS             complement(17573..18190)
                     /gene="tdk"
                     /locus_tag="ACHKJF_002438"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP0949783.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            18795..19208
                     /gene="hns"
                     /locus_tag="ACHKJF_002439"
     CDS             18795..19208
                     /gene="hns"
                     /locus_tag="ACHKJF_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP0949784.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(19352..20260)
                     /gene="galU"
                     /locus_tag="ACHKJF_002440"
     CDS             complement(19352..20260)
                     /gene="galU"
                     /locus_tag="ACHKJF_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP0949785.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(20462..21475)
                     /gene="rssB"
                     /locus_tag="ACHKJF_002441"
     CDS             complement(20462..21475)
                     /gene="rssB"
                     /locus_tag="ACHKJF_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP0949786.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(21567..22472)
                     /gene="rssA"
                     /locus_tag="ACHKJF_002442"
     CDS             complement(21567..22472)
                     /gene="rssA"
                     /locus_tag="ACHKJF_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP0949787.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            22585..23043
                     /gene="ychJ"
                     /locus_tag="ACHKJF_002443"
     CDS             22585..23043
                     /gene="ychJ"
                     /locus_tag="ACHKJF_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP0949788.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            23093..23935
                     /gene="purU"
                     /locus_tag="ACHKJF_002444"
     CDS             23093..23935
                     /gene="purU"
                     /locus_tag="ACHKJF_002444"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP0949789.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            24095..24179
                     /locus_tag="ACHKJF_002445"
     tRNA            24095..24179
                     /locus_tag="ACHKJF_002445"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24129..24131,aa:Tyr,seq:gta)
     gene            24214..24298
                     /locus_tag="ACHKJF_002446"
     tRNA            24214..24298
                     /locus_tag="ACHKJF_002446"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24248..24250,aa:Tyr,seq:gta)
     gene            24346..24477
                     /locus_tag="ACHKJF_002447"
     ncRNA           24346..24477
                     /ncRNA_class="other"
                     /locus_tag="ACHKJF_002447"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(24661..25338)
                     /gene="narI"
                     /locus_tag="ACHKJF_002448"
     CDS             complement(24661..25338)
                     /gene="narI"
                     /locus_tag="ACHKJF_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0949790.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(25338..26048)
                     /gene="narJ"
                     /locus_tag="ACHKJF_002449"
     CDS             complement(25338..26048)
                     /gene="narJ"
                     /locus_tag="ACHKJF_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0949791.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(26045..27583)
                     /gene="narH"
                     /locus_tag="ACHKJF_002450"
     CDS             complement(26045..27583)
                     /gene="narH"
                     /locus_tag="ACHKJF_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0949792.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(27580..31323)
                     /gene="narG"
                     /locus_tag="ACHKJF_002451"
     CDS             complement(27580..31323)
                     /gene="narG"
                     /locus_tag="ACHKJF_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP0949793.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMTDKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         7950 a         8010 c         7485 g         8098 t
ORIGIN      
        1 ttgatctgga ttaatttaga agttgcattg agaatcctgg ttaatgtgat ttaaatcaat
       61 ttttaaatat tttctctacg caaatattaa ctcgatcgcc acttgccgcg gtttgcccac
      121 gcctgaaggc cagcaattac ctccgcttta caaagatatg cttttccata caagacctga
      181 tgctgccacc cgaggcttaa ccagggtaca attgcccgct aattaacacc tgcataaaac
      241 tcaaggagag tgcatgtcta tcacggcgca gtccgtatac cgtgacaccg gaaatttctt
      301 ccgtaatcaa tttatgacca ttctgttggt atcgttgcta tgtgcgttta tcacagtggt
      361 gttagggcat gttttctcac ccagtgatgc acagcttgcg cagctcaatg acggcgtgcc
      421 cgttagcggc agtagtgggt tgttcgacct ggttcagaat atgtcaccgg aacagcaaca
      481 aattttgctg caggcttcag cggcgtccac tttttcagga ttaatcggta acgccattct
      541 cgccggaggc gtaatattaa ttatccagct ggtgtctgcg ggtcagagag tcagtgcgct
      601 gcgagctatt ggtgccagtg cgccgatatt gcccaagtta tttattctga tttttctgac
      661 taccctttta gtacagattg gcatcatgct ggtggtcgtt ccgggaatta tcatggccat
      721 tttactggcc ctggcaccgg tgatgctggt acaggacaaa atgggcattt ttgcctcgat
      781 gcgtagcagt atgcggctga cttgggcgaa tatgcgtctg gtggcacccg cagtactgag
      841 ctggttgctg gcaaaaacac tgttgctgct ttttgcctct tcttttgccg cattaacccc
      901 ggaaattggt gccgtactgg cgaacacctt gagcaacctg atttctgcca tattgctcat
      961 ctatctgttc cgcctgtata tgttgattcg ccaataacct taagcatttg attacggaat
     1021 cgtaaaatga agcagtttct tgatttttta ccgctggttg tctttttcgc gttttacaag
     1081 atttatgaca tctacgcggc tactgcggcg ctgatcgtcg ccacggcgat tgtgcttatc
     1141 tatagctggg ttcgctttcg taaggttgag aagatggccc tgatcacttt tgttctggtg
     1201 gtcgtcttcg gtggcttgac gctgttcttc cacaatgatg agtttattaa atggaaggtt
     1261 acagtcattt atgctctgtt tgcgggagcc ctgttagtca gccaatgggt gatgaaaaag
     1321 ccgctaattc agcggatgct gggtaaagaa ctcacgctgc cgcaaccggt atggtcgaag
     1381 ctaaatctgg cttgggctgt tttctttatc ctttgcggtc tggcaaacat ctacatcgca
     1441 ttctggctgc cgcaaaatat ttgggtcaac tttaaagtct ttggcctgac cgcccttacc
     1501 ttaatcttca cattgttaag cggtatctat atctaccgcc acatgccgca ggaagataaa
     1561 tcctaaccag actgccagtc cttcggggct ggcgctgtat ctcctctgtt ctcctcagga
     1621 aaatcatagt agcatcgcgc ctgtgatttt ccttttaagt cggttttacc atgtctacaa
     1681 cacataacgt ccctcagggc gatcttgttt tacgtacttt agccatgccc gccgatacca
     1741 atgccaatgg tgacatcttt ggtggttggt taatgtcaca aatggatatt ggcggcgcta
     1801 ttctggcgaa agaaattgcc cacggtcgcg tagtgactgt gcgggttgaa ggaatgactt
     1861 tcttacggcc ggttgcggtc ggcgatgtgg tgtgctgcta tgcacgctgt gtccagaaag
     1921 ggacgacatc ggtcagcatt aatattgaag tgtgggtgaa aaaagtagcg tctgaaccaa
     1981 tcgggcaacg ctataaagcg acagaagcat tatttaagta tgtcgcggtt gatcctgaag
     2041 gaaaacctcg cgccttacct gttgagtaat agtcaaaagc ctccggtcgg aggcttttga
     2101 ctttctgctt actgaatttc ggtggtgccg ttaattttaa acaggatatt caccacaatc
     2161 ccactgcctg gcttacccgg ctcataacgc catctgcgca tcgcattttt cacctcacgc
     2221 tcaaacatgt tcgcaggctt ggctgagagg atttgtacgt tatccacgcg accatccggc
     2281 gtgacgtcaa atttaacttt aacctgccct tcaatgcgca atgcctgtgc tcgtgccgga
     2341 tactgcggct gattacggct taatgcgcgt ggtcctgaag ccacactggt aaccggcttg
     2401 ctggttgcag ccgttgccgt acttgatgtc aggcgcgcgg gtgccgtatt ttcaaacggt
     2461 gatgccggac gcgactctac gggtttgata tcgcgttttg gctgctcctg tacctttttc
     2521 accggcttcg gttttggctt aggtttcggc ttcggctttt caatgaccac cggtgcttct
     2581 tttggcggtt cggggatcgg ctcaggttcc ggttctggct ctaccaccgg ctccggtggc
     2641 ggctgaacgg cttgtggcgg ttcgagatca gcaggcgcaa ccatcgtgac agaaattggc
     2701 tgcgcaggcg caggtagttc aataacctga tgtaccgagg tatagagcag acccgccaca
     2761 acagcaccat gaatgcagac cgaaagtaac gtcggccagg ggaagcggcg aggtaaatca
     2821 agggtcattg aagtcataat catttcagta gaaaaaccag gtctcgattt taaatgcaaa
     2881 tagcaatcat attcaataag gcaagacgat caggatcagt gaaaatgtca tttttggcgt
     2941 aattgacctt actttatgca tggtcttaac aaagacatca tctttccgtt gcagatcttc
     3001 gccctttcaa ataacgtact ttacaacttt cccgaacaag gagttgtgcc cgtgttgtat
     3061 gttatttacg ctcaagacaa agctgatagc ctcgaaaaac gcctttccgt tcgtccggca
     3121 catttagcac gtttacagtt actgcatgat gaaggtcgtt tgttgacagc tggtccaatg
     3181 ccagcggtag acagtaacga tccaggggct gcgggattta ctggctcaac cgttattgct
     3241 gaatttgaat cgctggaagc agctcaggcc tgggccgatg ctgacccgta cgtagcggca
     3301 ggtgtctatg aacacgtttc agtgaaacca tttaagaaag tgttctgaat agagattcaa
     3361 tatttaaggg cacggttttt gcaaccgtgt tcttgtttgg cctttcctta tagccggtca
     3421 gtgcattcac taccacaagc agataataac tttatctgta tttctttata tgatgtgaac
     3481 atattcagga aaatgccctt tcctgacgca gagttgctcg ctcaaaacct ttgattactg
     3541 ccgataaagt catctactat tgtttaacac attcacgcgc tgcatgtatg ttcagaaaat
     3601 gtgcacgcta agataattaa acaaaattgt ggttacagag gtatcagtga gtcactgggc
     3661 tacattcaaa caaacagcaa caaatttatg ggtgacccta cggcacgaca ttctcgcgct
     3721 ggccgtcttt ttaaatggat tgcttatttt taaaacaatc tatggtatgt cggtcaattt
     3781 gcttgatatt ttccatatca aagcattttc agagttggat ctctccttgc tggcaaacgc
     3841 cccactattt atgctcggcg tctttcttgt cctgaactcc attggcttac tgttccgggc
     3901 aaagctcgca tgggcaatca gtatcatttt gttgttgata gcgctaattt acaccctgca
     3961 tttttatccc tggctgaaat ttagtattgg attttgcatt tttacgctgg tgtttttgct
     4021 gatactgcgc aaagacttct cccacagtag cgccgcagcc gggacaattt ttgcatttat
     4081 tagtttcacg acattactgt tttactcaac ctacggtgcg ctttatttaa gcgaaggttt
     4141 taatccgcga atagaaagtt tgatgaccgc gttctatttt tcgatagaaa ccatgtcaac
     4201 cgtcggctat ggcgatattg tccctgtttc tgaatcagca cgattgttca ctatttcggt
     4261 cattatttcc ggcattaccg tttttgccac atctatgacc tcaatttttg gtccgcttat
     4321 ccgcggggga ttcaacaaac ttgtaaaagg aaacaatcat acaatgcatc gtaaagatca
     4381 ttttattgtt tgcggacatt cgattctcgc catcaatacg atcctgcaac tgaatcaacg
     4441 cggacaaaac gtaacggtta tcagcaactt gcctgaagat gatatcaagc aacttgagca
     4501 acgcttaggc gataacgctg atgttatccc cggtgacagt aatgacagtt cagtattaaa
     4561 gaaagcggga atcgatcgtt gccgggccat tctggcgctg agtgataacg atgcagataa
     4621 cgcgtttgtt gtactctcgg caaaagatat gagcagtgat gtcaaaacag ttctcgccgt
     4681 cagtgatagc aaaaacctga ataagattaa gatggtacat ccggatatca ttctctcacc
     4741 gcaactgttt ggcagcgaaa ttctggcgcg agttttaaat ggtgaagaga ttaataatga
     4801 tatgctcgtt tcaatgttgt tgaactccgg tcatggcatt ttcagcgata acgatgaaca
     4861 agaaacgaaa gctgacagta aagaatcagt gcaaaaatag agtttaaaga cgaaagagta
     4921 aacttattgg gcaccgcaat ggtgcccagt atatttagat attgtagatc gcaaacaata
     4981 acgagttacg ctgtttgttt agaatgcact tcctgatgct gtggatacgc atatttcggc
     5041 gcgattgccc ctcaagccaa cgcgatttac gtcggctagc ctgacgctgc atgcgccagc
     5101 gccccacttc cgttctactc cgcttcatgt taactactct atgcaataac agaatggtca
     5161 ttataccccc acccccgacg ctgaccagtg cttttcccgc gtttttattt gctagatgaa
     5221 tccatatgcg taaactcata acaatgcgct ttcaaaagga tttctaatct atgacaaccg
     5281 tttatacgtt ggtgagttgg ttggccattc tgggatactg gttgctcatt gcaggcgtaa
     5341 ctttacgcat tctaatgaaa cgacgcgcag ttccctccgc gatggcctgg ctgttgatta
     5401 tttacattct gccgttagtc ggaattattg cctatcttgc cgttggcgag ctccatttag
     5461 gcaaacgccg cgctgagcgc gccagagcga tgtggccttc caccgcaaaa tggcttaacg
     5521 accttaaagc ctgtaagcat atcttcgccg aagaaaatag cagtgtcgct gcgccattat
     5581 tcaagctttg cgagcgtcgt caggggatcg ctggggtcaa agggaatcag ctacaactga
     5641 tgaccgagtc agatgatgtg atgcaggcgt taatccgcga catccagctc gcgcgccata
     5701 atattgagat ggtgttttat atctggcagc ccggcggcat ggcggaccag gtggctgaat
     5761 cattaatggc ggctgcacgg cgtggtattc attgccgatt gatgctcgac tccgccggga
     5821 gtgtggcttt tttccgcagc ccgtggcccg agctaatgcg taatgccggt attgaagtgg
     5881 tcgaagcctt aaaggtcaat ctgatgcgtg tgtttttacg ccgtatggac ctgcgccaac
     5941 atcgcaagat gatcatgatc gataattaca tcgcgtacac cggcagcatg aatatggtcg
     6001 atcctcgcta cttcaaacaa gatgcgggcg tagggcaatg gattgatctg atggcgcgta
     6061 tggaaggccc catcgccacc gcgatgggga ttatttattc ctgcgactgg gagattgaaa
     6121 ccggaaaacg tattctgccg ccaccaccag atgtcaatat tatgccgttt gaacaggcca
     6181 gcggtcacac cattcacaca attgcttctg gccccggctt tccggaagat ctcattcacc
     6241 aggcattatt gactgcggct tattcggcgc gtgaatattt gatcatgacc acgccctact
     6301 ttgtgccaag cgatgattta cttcatgcga tttgcacggc ggcgcagcgc ggggtggatg
     6361 tcagtattat ccttccgcga aaaaatgact cgatgctggt cggctgggcc agtcgcgcat
     6421 tctttacgga actgcttgct gctggggtta aaatttatca gtttgaaggc gggttactgc
     6481 ataccaagag cgtgctggtc gatggcgaac taagtctggt tggcaccgtt aaccttgata
     6541 tgcggagtct gtggctgaat ttcgagatta ccctggcaat cgacgataaa ggttttggtg
     6601 cagaccttgc cgccgttcag gacgattata tttcgcgttc acgtctgctc gatgcccgtt
     6661 tatggctaaa acgtccatta tggcaacgtg tcgccgagcg actgttttac ttcttcagtc
     6721 cgttgctgta aaacgtcgcc aacagaggtt aaacaggtag tgactatgga tatggatcta
     6781 aacaatcgcc tgactgaaga tgaaacgctt gagcaggcct acgatatttt tctcgaactg
     6841 gctgccgaca atctcgatcc agctgatgtt ctgctgttca atcttcagtt tgaagagcgc
     6901 ggcggcgcgg agttattcga tccggcggaa gactggcagg aacatgttga tttcgatttg
     6961 aacccagact tttttgccga agtggtgatt ggtctggcgg acagtgaaga tggtgaaatc
     7021 aacgatgttt tcgcccgcat tctgttatgc cgcgaaaaag atcacaaact ttgccatatc
     7081 atctggcggg aataaataat cgctttcggc gtgaaaaagg gctgacaact gtcagccctt
     7141 attgtgttta aagcggatcg actttcaggc aagaaacggc gtgtctgaaa ctcccctcca
     7201 gaacaggacg tgttttggcg cactccggac cggcaatcgg gcaacgggta cggaaaacac
     7261 aaccggaagg cgggttgatc ggcgacggta attccccttc cagtaactgg atggttttgt
     7321 tcttctccag atccggatca ggtatgggga ctgccgacat caatgccctg gtgtaaggat
     7381 gtagtggatt gtggtagacc tcatcatagg tccccagttc taccgcatgg ccgagataca
     7441 tcaccaacac acgatcggaa atgtgtttta ccacggccag gtcatgagcg ataaaaatta
     7501 atgacaatcc catctcacgt tgcagctgct ggagcaggtt gaccacctgc gcctgaattg
     7561 acacgtccag cgccgaaacc ggctcatcgc agataatcag cttcggttca agaataagag
     7621 cacgagcaat cccgatacgc tggcactgcc caccggagaa ctcatgcgga tagcggttaa
     7681 tcaggttagg caataacccg actttcagca tcatcgcctt cacgcgctcg cgaacttcct
     7741 ggcgtgacat tttcggatga taagtacgca gtggttcagc gatgatctcg ccgatggtca
     7801 tacgcgggtt tagcgatgcc aacggatcct ggaaaatcat ctgaatatca ctgcgaacgg
     7861 cacgccattc atcgggcttc atgcccagca actctttacc taaccaggca acatgaccgt
     7921 cggtcgcctt gaccaaaccg atgatggcgc gagcaaaggt ggacttaccg catcccgatt
     7981 cccctaccac acctaatgtt tccccttcat acaggcgaag agtgatacca tcgacggctt
     8041 tgagcgtttt cggcggttgc cagaaccact gtttgccatc tttgatttca aagtgcactt
     8101 taagatcggc gatttcaagg aggacttttc ttccttcagt tacagcattc ataacagttc
     8161 ctccaccggt ttaaagcaag cacgcagacg gccaggcgta aactcttcca gcggcggtgc
     8221 gctactacaa atttccatcg catgtggaca acgtggctgg aacgggcaac ctttcggtaa
     8281 tcgcagcaag tttggcggat tacccgggat ggtcaacatt gtttcacctt ccgcatcaag
     8341 acgcggcacc gcgttgagca aaccgataga ataaggatga acgggttgat aaaagacatc
     8401 gcgcgcattg ccatattcca tcgtgcgccc ggcgtacatt accagcactt tgtcacagat
     8461 ccccgccacc acgccaagat cgtgggtgat cataatgatg gcggtattaa attcccgctt
     8521 cagttcattt aatagcgtca tgatctgcgc ctgtacggtg acgtccagcg cagtagtggg
     8581 ttcatccgca atcagtagct taggtcgaca tagcaatgcc atcgcaatca tgactcgctg
     8641 acgcatgccg ccagaaaatt cgtgcgggta cattttcatg cgtttacgcg cttccggcat
     8701 ttttaccgca tcgagcatcc gcaccgactc ttcaaacgcc tcagctttgc tcatgttctt
     8761 atgcagcatc agcacttcca ttaactgctc accgacgcgc atatagggat tcaacgaagt
     8821 cattgggtcc tgaaaaatca ttgagatttg ttcagcgcgc agtttattga gttcatgctc
     8881 tggcaaattg aggatttcac gcccattgaa ggttgccgat ccgccaatac gtccgttggc
     8941 agccaacagg cccatcaacg caaatgcagt ttgcgattta cccgaaccag actcacctac
     9001 aatacccagc gtttctccgg cacgtaggga aaaattcaaa tcattgaccg ccgtgacgtc
     9061 gccgtccggg gtactaaagg tgacacgcaa atctttcacg ttcagcagtg cgtcagcctg
     9121 ttgttgtgcg agcggcacag ttgcagtttc aattacgctc atggctgcac tccttaacga
     9181 tctttcgggt cgagggcatc acgcaagcca tcgccgataa agttgaaaca aaacagcgtc
     9241 accacgagga atcccgctgg gaacaacaat aaccatggag agacttccat cgagttcgcg
     9301 ccatcactca gcaatgcacc ccagctgctt aacggctctt gcgtacccaa ccccaggaag
     9361 ctaaggaaag attcaaagag gatcatgctg ggcaccagaa gtgatgcgta gaccaccacc
     9421 acaccgagta cattcggcac aatgtggcga ataacaatgc ccgatgtcga tacaccgcca
     9481 acttgtgccg cctcaataaa ctctttgcgc ttcagactca gggtttgccc acgcacaata
     9541 cgagccatat ccagccagga aaccatgcct atcgccacga aaatcagcag gatgttttga
     9601 ccgaaaaagg tcaccagcaa aatgacgaag aacatgaatg ggaaggagtt gaggatttcc
     9661 agcagacgca tcattaccga atccacttta ccgcccagat aaccggaaag cgaaccataa
     9721 agtgtcccga cgaccacagc caccagtgcc gcagcaacac cgaccatgag tgagatacgc
     9781 ccgccaatcg caacgcgcac aagcaggtcg cgaccggatg agtcagtacc aaagtagtga
     9841 ccggactcca tatccggggc gctggacatc atcgcccagt cagtatcgtc ataggcaaac
     9901 tgggaaagca tcggtgccag gattacaaat aacgcgatca gcaccagcac tatcagactg
     9961 gcaaccgccg cacggttatg aataaaacgt cgacgtgcgt cctgccacaa gctgcgccct
    10021 tcgacctcca gcttttcact gaaattttcc agcgtctcgc tgtttttctt acttaacatc
    10081 atcgcgagct ccagtatcag taacggattt tcgggtcgat aaccgcatat agcacatcga
    10141 caatggcatt aaacaaaatg gttaaagcac caaccaggat ggtcaggctt aacactaagg
    10201 aatagtcacg gttcaatgcg ccattaacga acagttgccc aatccccggc aaaccataaa
    10261 tggtttcgat gaccatagaa ccggtaataa tgccgacaaa tgcggggccc atataggaga
    10321 gcacgggtaa cagagcaggt tttaatgcgt ggcgtaaaat gatccgccgc ataggtaacc
    10381 ctttcgcccg ggcagtacga ataaagttgg agtgtaatac ttcaatcata gagccacggg
    10441 taatacgcgc aatactggcg atataagcga gtgacaacgc caccatcggc aatatcatga
    10501 atttaagagc cccaccattc cagccaccgc ccggcagcca atgcaaaatg atcgcaaata
    10561 tcatgactaa taatggcgca accacaaaac tggggataac aaccccggtc attgccagcc
    10621 ccatcacggt atagtcccat ttggtgtttt gttttaatgc ggcaataacg ccagcactaa
    10681 cacccagtat taccgcaagg aaaaatgctg cggctcccag tttggcagaa acgggaaaac
    10741 tggatgcaac caggtcattg accgaataat ctttatattt aaacgatgga ccgaaatcac
    10801 cgtgcgccag ttgtttcagg tagctgaaat actgtgtcat gatcggatca ttaagatgat
    10861 atttcgcttc gatattggcc atcacttcag gcggtaaagt acgttcgccg gtaaaagggc
    10921 ttcccggcgc gaggcgcatc ataaagaacg aaatagtaat aagaataaat agcgtcggaa
    10981 tcgcttccag acagcgacgt agaataaatt ttaacattgc cctaccttct ggcctgtgcc
    11041 ttctgtaatg cgataaaaat cagacaccgt ggagcaggac actcctgccc cacgtattgc
    11101 cattagtgct tcacaatgta catattccgg gtgtaggtat tatccagcgg atctttgccg
    11161 gtatagccac caacccacgg tttcaccaga cgcgcattca cgtagtaata aacaggaaca
    11221 atggccgaat ccttatccag ctgttgttct gctttagtgt acagagctgt gcgctgcgcc
    11281 tcatcagtca ctttcagcgt ttccgccata atgctgtcaa aggccgggct cttataatgc
    11341 gcggtattca tcgagctgtt cgaaagcatg gtgttcagga aggaagttgg ttcgttgtag
    11401 tcagcacacc agcctgcacg ggccacatca aaagtaccct ggtgacgggt gtcgaggaac
    11461 gttttccact cctggttaac cagtttgacg tttacaccaa tgtttttctt ccacaatgaa
    11521 gaggcagcaa tcgccagctt tttatgcaga tcggaggtgt tatacaacag gttgatggtc
    11581 aacggtttgt ctgcggtata acccgcttca gccagcagtt ttttcgcttc ttcgttacgt
    11641 ttttcctggc tccagccaaa ccattccggc tgagtcaatt ttgcgccgtc agtatacggt
    11701 ggagtgtaac cataggcggg catgtcgccc tgcgctttca ctttattaac aatgatatcg
    11761 cggtccatac ctagtttcag tgcggtacgc acacgcacat cgttgaatgg cggtttctgg
    11821 ttgttaattt cgtaatagta agtgcacagg tatggatcaa cgtgaacttc gtccgggatc
    11881 tcttttttca gcttctggaa caattcgatc ggcatgttgt tataagtcat gtcgatttca
    11941 ccactacggt aacggttgac atcggtaact tcagaagcaa taggcaaata ggttacctgg
    12001 ttaataacgg ttttcgcgtt gttccagtag gtcgggctgc gttcaagaac gattcgttcg
    12061 tttacgaccc aatcttttaa ggtataggca ccgttggtga cgatattacc aggctgggtc
    12121 catttttcgc cgaatttctc gatagcggct tttggcaccg gtgaagttga tgggtgaaca
    12181 agtaatttat agaagtacgg aacgggttca cttaaggtga cttctaatgt gtgatcatca
    12241 atagctttca cgccgagatc ggtaatcggt tttttccctt caagaatttc atcaataccg
    12301 gcgatatgcc catattgcag ataactggca tacggagaag cagtgttcgg atcaacagaa
    12361 cgttgccagc tatacacaaa gtcttgtgct gtgactggcg tgccatcaga ccatttcgca
    12421 tctttacgca aatggaaggt ccagactttc gcgtctttat tatcccagga ttcagcgacg
    12481 ccaggtgctg gatgaccgtc aagatcgctg accagtaagc cttcaaacag gtctcggctg
    12541 atattagact ccggaacacc ttcaattttg tgcggatcta atgactgaac ttctgaacca
    12601 ttgttacgta ccagtgtttg tttttccgcc agtgtgacgc ctgcgggtac atcagctgcc
    12661 agcgcgacat tccctgccat tagcgcagcc agaacgccag ctgctactaa acttctcttg
    12721 gtgatgttgg tcattgtttt ttggactccc tcattataat tactggtatt aaccagcatg
    12781 tgtaatcccg gattgggacc ctgcacaggc tcggagactt tctgctgtca ggttacttta
    12841 tcgttacgta ttactatcac cgactttatt tattctggtg attcagacga tcaccttatg
    12901 tcgattcgta ctgtgtctcc cctgtcgaga cacattattc tttcgcgacg ctctttaatt
    12961 gttaattatt ctcattacca ataaagaatc gtctggcggt tgcccggaaa gtaccaaatg
    13021 cggttcttac tcgccaatac attttgcaaa attgttaagc aattctcttt tattgaccgt
    13081 atcagaatct atagcttgca tccatgcctt aacaaacatc tttcaattca atcaaataga
    13141 gattggctaa gcatgtctat cttttctgat tacagttcca gtagtgaaat gcacaataat
    13201 ttaacaattg attattattt agcactttcc tctaccaaag gcagtggaat tactaatatc
    13261 atttcaatta ttcttcagca agcccaggat tatgatgtgg cgaaaataac atgatggtga
    13321 ggagaagcag ttaaggaaat ccttataact aactgtaatt tgagaagaat atttttacct
    13381 attccttttg cttttgataa ctgcgcggga acaaagtaaa aataatgacg aagtaaacgg
    13441 agcatggcag ctccgtttca tttgaaagga attaattaag caggccgggg aaaatcccct
    13501 taataccagt aacgataaat tcaatcccca atgccatcag caatagcccc ataatacgcg
    13561 taatcacgtt gatgccggtc tggcgtaaaa cccgtaccag ccacggtgcc atgcggaaca
    13621 atccccagca acataaagcg aacaatgcaa tagccacaaa gaaaccaaac agatagctaa
    13681 tgctgtgata acgcgtaccc cagacgatgg tagaactgat cgcccctggc cccgccatca
    13741 acggcaacgc caatggcacc acaccaatgc tttcacgtac cgcggtttct gatttttctt
    13801 gcttgttctg tttatcctcg ccaagcttgc cgctgatcat cgacatcgct atcgtcacca
    13861 ccaggatgcc cccggcgata cggaacgaat caattgatat accaaaaagt tgcagaatcg
    13921 tgtcgccgag aaaaagcgag atccacaaga taatggccac agacaggttg gctgtaaggt
    13981 tagttttgtt tcgcgctgcc gctgtctgat aactggtcat gctgataaag acgggaataa
    14041 tccctaccgg gttgaccagc gcaaataacc cgatgaaaaa tttgaagtaa acgggaaaat
    14101 caaaaaaggt ctgaatcacg gttagctccg aagcaaaagc cggataatgt tagccataaa
    14161 taaggttgaa aagacgcgct gacaatacgc cttttgacag catttttcac ctcctaacta
    14221 cttaaaattg ctatcattcg ttattgttat ctagttgtgc aaaacatgct aatgtagcca
    14281 ccaaatcata ctacaattta ttaactgtta gctataatgg cgaaaagcga tgctgaaagg
    14341 tgtcagcttt gcaaaaattt gatttggatc acgtaatcag tacccagaag tgagtaatct
    14401 tgcttacgcc acctggaagt gacgcattag agataataac tctaatgttt aaactctttt
    14461 agtaaatcac agtgagtgtg agcgcgagta agcttttgat tttcataggt taagcaaatc
    14521 atcaccgcac tgactatact ctcgtattcg agcagatgat ttactaaaaa agtttaacat
    14581 tatcaggaga gcattatggc tgttactaat gtcgctgaac ttaacgcact cgtagagcgt
    14641 gtaaaaaaag cccagcgtga atatgccagt ttcactcaag agcaagtaga caaaatcttc
    14701 cgcgccgccg ctctggctgc tgcagatgcc cgaatcccac tcgcgaaaat ggccgttgcc
    14761 gaatccggca tgggtatcgt cgaagataaa gtgatcaaaa accactttgc ttctgaatat
    14821 atctacaacg cctataaaga tgaaaaaacc tgtggtgttc tgtctgaaga cgacactttt
    14881 ggtaccatca ctatcgctga accaatcggt attatttgcg gtatcgttcc gaccactaac
    14941 ccgacttcaa ctgctatctt caaatcgctg atcagtctga agacccgtaa cgccattatc
    15001 ttctccccgc acccgcgtgc aaaagatgcc accaacaaag cggctgatat cgttctgcag
    15061 gctgctatcg ctgccggtgc tccgaaagat ctgatcggct ggatcgatca accttctgtt
    15121 gaactgtcta acgcactgat gcaccaccca gacatcaacc tgatcctcgc gactggtggt
    15181 ccgggcatgg ttaaagccgc atacagctcc ggtaaaccag ctatcggtgt aggcgcgggc
    15241 aacactccag ttgttatcga tgaaactgct gatatcaaac gtgcagttgc atctgtactg
    15301 atgtccaaaa ccttcgacaa cggcgtaatc tgtgcttctg aacagtctgt tgttgttgtt
    15361 gactctgttt atgacgctgt acgtgaacgt tttgcaaccc acggcggcta tctgttgcag
    15421 ggtaaagagc tgaaagctgt tcaggatgtt atcctgaaaa acggtgcgct gaacgcggct
    15481 atcgttggtc agccagccta taaaattgct gaactggcag gcttctctgt accagaaaac
    15541 accaagattc tgatcggtga agtgaccgtt gttgatgaaa gcgaaccgtt cgcacatgaa
    15601 aaactgtccc cgactctggc aatgtaccgc gctaaagatt tcgaagacgc ggtagaaaaa
    15661 gcagagaaac tggttgctat gggcggtatc ggtcatacct cttgcctgta cactgaccag
    15721 gataaccaac cggctcgcgt ttcttacttc ggtcagaaaa tgaaaacggc tcgtatcctg
    15781 attaacaccc cagcgtctca gggtggtatc ggtgacctgt ataacttcaa actcgcacct
    15841 tccctgactc tgggttgtgg ttcttggggt ggtaactcca tctctgaaaa cgttggtccg
    15901 aaacacctga tcaacaagaa aaccgttgct aagcgagctg aaaacatgtt gtggcacaaa
    15961 cttccgaaat ctatctactt ccgccgtggc tccctgccaa tcgcgctgga tgaagtgatt
    16021 actgatggcc acaaacgtgc gctcatcgtg actgaccgct tcctgttcaa caatggttat
    16081 gctgatcaga tcacttccgt actgaaagca gcaggcgttg aaactgaagt cttcttcgaa
    16141 gtagaagcgg acccgaccct gagcatcgtt cgtaaaggtg cagaactggc aaactccttc
    16201 aaaccagacg tgattatcgc gctgggtggt ggttccccga tggacgccgc gaagatcatg
    16261 tgggttatgt acgaacatcc ggaaactcac ttcgaagagc tggcgctgcg ctttatggat
    16321 atccgtaaac gtatctacaa gttcccgaaa atgggcgtga aagcgaaaat gatcgctgtc
    16381 accaccactt ctggtacagg ttctgaagtc actccgtttg cggttgtaac tgacgacgct
    16441 actggtcaga aatatccgct ggcagactat gcgctgactc cggatatggc gattgtcgac
    16501 gccaacctgg ttatggacat gccgaagtcc ctgtgtgctt tcggtggtct ggacgcagta
    16561 actcacgcca tggaagctta tgtttctgta ctggcatctg agttctctga tggtcaggct
    16621 ctgcaggcac tgaaactgct gaaagaatat ctgccagcgt cctaccacga agggtctaaa
    16681 aatccggtag cgcgtgaacg tgttcacagt gcagcgacta tcgcgggtat cgcgtttgcg
    16741 aacgccttcc tgggtgtatg tcactcaatg gcgcacaaac tgggttccca gttccatatt
    16801 ccgcacggtc tggcaaacgc cctgctgatt tgtaacgtta ttcgctacaa tgcgaacgac
    16861 aacccgacca agcagactgc attcagccag tatgaccgtc cgcaggctcg ccgtcgttat
    16921 gctgaaattg ccgaccacct gggtctgagc gcaccgggcg accgtactgc tgctaagatc
    16981 gagaaactgc tggcatggct ggaaacgctg aaagctgaac tgggtattcc gaaatctatc
    17041 cgtgaagctg gcgttcagga agcagacttc ctggcgaacg tggataaact gtctgaagat
    17101 gcgttcgatg accagtgcac cggcgctaac ccgcgttacc cactgatctc cgagctgaaa
    17161 cagatcctgc tggataccta ctacggtcgt gattatgtag aaggtgaaac tgcagcgaaa
    17221 aaagaagccg ctccggctaa agctgagaaa aaagcgaaaa aatccgctta atcagtagcg
    17281 ctgtctggca atataaacgg ccccttctgg ggccgttttt ttgtttaccc aaagcaactt
    17341 ttccataaac cgacagcatt agccttcatc atatttgcga cgatgtataa cgcctaaaca
    17401 cagggatatt gtactttaca ggtcacaagt caacgtcggt gcttaagagc cctgtgaggc
    17461 gtatagcggc gttaaaaaac tgccgagaag ggtatatagc ccggaagaag tgcgtaaaac
    17521 gaactgacag gataaaagtg ccctgctcac cctgtcagta aagaaattct tattaatcgt
    17581 ggcgatgcct ttcctgaata gccgttaatg agccgacttg taacgcctct ttatagtgtt
    17641 tacggcatac agaaacgtat cgttcattac caccaattac cacctgctca ccttcgttat
    17701 aaggtctgcc tgcttgatca agacgcagca ccatgcttgc tttacggcca caaaaacaga
    17761 tggtttttaa ttcaaccagt ttgtcggacc atgccagtaa gtattggctg ccaataaata
    17821 attcacctcg aaaatcggta cgtaaaccat aacaaagtac gggtatatcg agttgatcga
    17881 caacctccga taattcatat acttgttgtc tggttaaaaa ctggcattca tcaaccagta
    17941 cgcaatgaat tgcctgctgt tcatgttccg cacgaatctc atcaaataat gatgaatttt
    18001 ggttaaataa ttttgcaggc gatgacaaac ctatacgcga actgactttc ccggcaccaa
    18061 agcgatcatc aatttctgcc gtatatacga cagtgcgcat gccgcgttcc tggtaattgt
    18121 atgaagattg caacaatgct gtagacttac ccgcattcat tgcggaatag tagaaatata
    18181 gctgtgccat cagccacagg ccctcaatga tatgaaataa agttggctgg agtttatcat
    18241 aattcgtcag tttttcagct caacagtatg cacagaagtg ggtatatgcg ttctccctta
    18301 cgaagccttg cataatcctt ctgagctatc attacaactg cctcgcttgt tataagcggg
    18361 taaatgactg ctggtaacta ttcacaatct ttaacctgtt gcgcaagtaa tagccctctg
    18421 ttgacctcca ggagatagtg caatactaag tccatgctct tattgcgact gttctacttt
    18481 tcatcattcg cttaataggg aattctcgta aacacaacta atacagaaga ctgaaaggtc
    18541 gtcagcctac gataatctcc ccataaaatg tgacatgaat caggaagttt taacctcacg
    18601 tgctgcgaaa tcatcggtgt aaatagggct atatgccgcg tcttttctgg ctaattttat
    18661 gaaaagatat ttattggcgg cacaaaataa agaacaattt tgaattcctt acattcctgg
    18721 ctattgcaca actgaattta aggctctatt attacctcaa caaaccaccc caatataagt
    18781 ttgagattac tacaatgagc gaagcactta aaattctgaa caacatccgt actcttcgtg
    18841 cgcaggcaag agaatgtaca cttgaaacgc tggaagaaat gctggaaaaa ttagaagttg
    18901 ttgttaacga acgtcgcgaa gaagaaagcg cggctgctgc tgaagttgaa gagcgcactc
    18961 gtaaactgca gcaatatcgc gaaatgctga tcgctgacgg tattgacccg aacgaactgc
    19021 tgaatagcct tgctgccgtt aaatctggca ccaaagctaa acgtgctcag cgtccggcaa
    19081 aatatagcta cgttgacgaa aacggcgaaa ctaaaacctg gactggccaa ggccgtactc
    19141 cagctgtaat caaaaaagca atggatgagc aaggtaaatc cctcgacgat ttcctgatca
    19201 agcaataatc ttttgtagat tgcacttgct taaaatcccg ccagcggcgg gattttttat
    19261 tgtccggttt aagacaattt aataagaata agctataaaa aaacggcgtc gattgctcaa
    19321 cgccgtttcg tggataacac cgatacggat gttacttctt aatgcccatc tcttcttcaa
    19381 gccaggcttt aaattccgtg ccaagggtgt tatgacgaat accgtattca acgaaggcct
    19441 gcatgtaacc taatttatta ccgcagtcat ggctcttccc tttcatatga taggcttcca
    19501 ccgtttcttt ttcgatcagc atatcaattg cgtcggtgag ctgaatttca tcaccagctc
    19561 ccggaggggt ttttgccagc aacggccaaa tatccgcgct aagtacgtaa cgacccacaa
    19621 tagcgagatt agacggcgca acatccgctt tcggtttttc taccacacca accatcggta
    19681 cgctttcacc cggcgctaat tcaacgcctt tgcaatccac aacgccatat gcggtcacat
    19741 cagcaaccgg ttcaaccatg atctggctat gacccgtttc atcaaagcgg cggatcatct
    19801 ctgccaggtt atcctgtgac aaatcggatt catattcatc cagaataaca tcaggcaaaa
    19861 taacagctac cggttcatca cccactaccg ggtgagcaca caataccgcg tgtcccaggc
    19921 ctttcgccag accctgacga acttgcataa tagtcacgtg cggtggacaa atagactgca
    19981 cttcatcaag cagttgacgt tttacacgtt tttccagcat tgcttccagt tcaaaactgg
    20041 tatcaaagtg gttttcaata gagtttttag atgagtgtgt aaccagcaca atttcagtaa
    20101 tgccagccgc aatacattca ttcacgacgt attgaattaa tggcttatcg acaagtggca
    20161 gcatctcttt cgggatggct ttcgtcgccg gcaacatcct ggttcctaat cccgcaacgg
    20221 ggataacggc ttttttgact ttcgtattaa tggcagccat ttaaattctc ctggactgtt
    20281 cgtgttttga acgtgttcat atttctgtat cgcatcccag tatatcagca ccccgaaggg
    20341 gctaagttct gaaaaggagg cgagttagca taattaagac aattacttat acgaatggcg
    20401 acgatagtac caccagccga cattagcagg taatgcaaat ttagcccgcg ttatcgtttg
    20461 ctcattctgc agacaacatc aagcgcagtc gaccaccggt tccccatatt tggcattgcc
    20521 aggcatcgca tcgctggctc aattgattca aataagcgtt acctaaagtg cctaacggaa
    20581 caccattact gatttgcacc tggtgttcgc cggtatttaa cgtcgcattc agacccgcag
    20641 aaaccagaat gagatttttc agttcgcgat gataatagcc aactaatagc ggaaactgcc
    20701 ccggcagatt ggcctgacga agtaaatggt ttacctgctt caataacgcg cccaactctg
    20761 gcaaccgttg attttggtgt gcaagctgtt cctgtaataa tccgttaaac aatgcgcgta
    20821 ataacaaggc agcaagtacg ccattatgtc cagctcgggt gacatcaagg caataaaatg
    20881 ccagatcgtt ttccgaaagt gcggcaatat caagcaccag gccgggtttg tccgcggcaa
    20941 ccaattgacg ataattaacc cggcaatggg aaatcacctg ctgaaccggc ggttgtagtt
    21001 cctgtaataa tttcgccgct gcggcagggt tatcaaccat tgcatcccag tcgcgaaaaa
    21061 gcctttcctc ttcctcaacg cgcgaattaa acatgctggg atagagacag gcaaaaacca
    21121 tctcgcgcaa gcgattcaga tctttaactg gtttcagcaa aacatcttca acgcccagac
    21181 gtaacgcttt ggcaatatct gccatatttt cagtggcaga tatcaccaga accggggtct
    21241 ggtcgcctct gttacgtata tgctccagca gtttaagccc gttcattcgt ggcatcgcga
    21301 tatcacatat catcaggtct ggagtgaaac ctcccagcaa ctcaagggca tccaccccat
    21361 cagccgccag taccgttgtc gctcccaatg aggaaaacca cgaatccaga agcgagcgaa
    21421 ataccggctc atcttcaaca atgagaatct gttttccgac caatggctgc gtcatgttct
    21481 ctcccctgac tggctttact caatagtggc atgctattgc cacctgcgcc tgtcagaatt
    21541 aacttaagtg tagataaaaa ttctggtcaa atgttggtgc gtaccaacgg caaaagttcg
    21601 tccattttct tttccactgc cagctgtccg gccgcaatgg cagcgtgcgc gcgatggaaa
    21661 tcaagcgtag atatttgcgg gcaaacaggt tgaatcagaa tatcgggcgg atcacctgcc
    21721 atgcggttcc ttttaaggcg gttctccagc acctggatag aagtggtcat aatctctgtt
    21781 gccgttggtg ctgtcaccgc ccgacgtgtc gttatgctgc ctaacctttc tttcagacgc
    21841 gcatgccacg gcagagaatc accattctcg ctattttctt cactgacatt aaaggagagc
    21901 aaatcttgtt gcattaaatg agcatcgtgc tgcaggtcga ccgctatcac aatatcagca
    21961 cccaatgcac gcgtgagcga aataggaatt gggttaacga ctgccccatc aaccagccag
    22021 tagccgttat gcgcgactgg tgccatgagt cctggaatac tgcatgacgc gcgaatagca
    22081 agatggagat cgccttcagt aaaccataat tcacgcccag tacttaaatt ggtggcaaca
    22141 gccgcgaagc gacgggaaca attttcgatc tctgtttccg gcattatctc gcggtattga
    22201 ttgaagacac gctcgccgcg cagtaaccca ccgcgctgcc aggagagatc catcaggcgt
    22261 aaaacatccc aataactgaa agaggttacc caatcttcca gcgcagataa tcgatcgcat
    22321 gcataggccg cgcccaccag cgaaccaatt gaacatcctg caacgatatc aatttcaata
    22381 cccacttttt ttagcgcatt aataacgcca atatgcgacc aacctctcgc cgcgccagat
    22441 cccagcgcca gccctatttt tatctttctc attattcccg caagattccc ctggaatgca
    22501 atcgtagcca catcgctact gctcggttaa catagtgcta ctcaggcggt taatacgccg
    22561 ttattgtttc cagggagatt atttgtgtct cagctttgtc cctgtggtag tgctgtcgag
    22621 tatagcctat gttgccaccc ttatgtgtct ggtgaaaagg ttgcacctga tccagaacat
    22681 ctcatgcgtt cgcgttactg cgcttttgtg atgcaagacg cagattattt aataaagacc
    22741 tggcatccat cttgtggagc agcggcgtta cgtgccgaat tgattgccgg atttgcgcat
    22801 actgaatggt tgggattgac cgtatttgaa cattgttggc aggacgccga caatatcgga
    22861 tttgtcagtt ttgttgctcg atttaccgaa ggtggcaaaa caggtgcaat aattgaacgt
    22921 tcgcgatttt tgaaagaaaa cggtcaatgg tactatattg acggtacacg tccgcagttt
    22981 ggccgcaacg atccctgccc ttgtggttca ggtaaaaaat ttaaaaagtg ctgcggccaa
    23041 taatggttga cggtacggtt tagcaaacac tctcaacaag gtttttccag caatgcattc
    23101 actccaacgt aaagttctgc gtactatttg tccggaccaa aaaggtctga tcgcacgtat
    23161 taccaatatt tgctacaagc acgagttaaa tatcgtgcag aacaatgaat ttgttgatca
    23221 ccgtaccggg cgctttttta tgcgcacgga actggaaggg atttttaatg attccaccct
    23281 tctggcggat ctcgatagcg cattgccaga aggctccgtg cgtgagctga atcctgccgg
    23341 tcgtcgccgg atagtgattc tggtcactaa agaagcgcat tgccttggcg atttgttgat
    23401 gaaagccaat tacggcggcc tggatgtcga aatcgcggca gttattggta accacgatac
    23461 tttacgttct ctggttgagc gttttgatat tccgtttgag ctggtaagcc atgaagggtt
    23521 aagccgcaac gagcacgatc aaaagatggc ggacgccatt gatgcttatc aacctgacta
    23581 tgtggtgctg gcgaagtata tgcgggtatt aacgccggaa tttgtggcac gcttcccgaa
    23641 taaaatcatc aatattcacc attcattcct gcctgcgttt atcggcgcac gtccttatca
    23701 ccaggcctat gaacgtggtg tgaagattat tggcgcaacc gctcactatg tgaatgacaa
    23761 tctggacgaa ggcccaatca tcatgcagga cgttattcat gtcgatcata cctacacagc
    23821 tgaagatatg atgcgcgcag gtcgtgacgt cgagaaaaac gtcttaagtc gtgcgctata
    23881 caaagtactg gcacagcgcg tctttgttta cggtaatcgg acgattattc tttaatcgcc
    23941 agcaaaaata actggttacc tttaatccgt tacggatgaa aattacgcaa ccagttcatt
    24001 tttctcaacg taacacttta cagcggcgcg tcatttgata tgatgcgccc cgcttcccga
    24061 taagggagca ggccagtaaa aagcattacc ccgtggtggg gttcccgagc ggccaaaggg
    24121 agcagactgt aaatctgccg tcatcgactt cgaaggttcg aatccttccc ccaccaccat
    24181 ctcaagtatt acctcaaaat ttgaattacc cttggtgggg ttcccgagcg gccaaaggga
    24241 gcagactgta aatctgccgt catcgacttc gaaggttcga atccttcccc caccaccatc
    24301 acttttcaaa agtccgtaaa ctctcaaacg aatccgcaat taaatattct gcccaagcgg
    24361 ggaaggatga gaagcttcga ccaaggttcg acttgagcgc cagcgagaga gcgttgccgc
    24421 aggcaacgac ccgaagggcg aagcgcgcag cgctgagtaa tccttccccc accaccatcg
    24481 cttccttaaa taatcaaaac ctgttactcc gagctaattt tgcccgatgg caccacgttt
    24541 accgggccta caatatcctc ccctgtaggt cggataagcg aagcgcatcc gacaactcac
    24601 cccgcaccgt gcccataaaa aaaccccgcc gaagcagggt ctatgtgaac taaaattcgc
    24661 ttagtgacga gcgcgcacca gctggtactt acgtgtcaga tactccaccg gtacgctcca
    24721 gatgtgtacc agacgcgaga acgggaacag caggaataac gtcatcccca gcaccaggtg
    24781 cagacggaag ataaacgcca caccatcgag gtgttgagaa gcgccaccgt ggaaggtcac
    24841 caccgactgc gcccagccaa ccagtttcat catctcgcta ccgtccatat gctgagcgga
    24901 gaacggaatg gtcaacaggc ccagcgcgca ctggataacg agcagcgaca ggatcaggat
    24961 atccgctccg gtagtggttg cacgcacgcg tgggctgaac agacgacgct tcagcaacag
    25021 cacgccgcca atcagacaca gcacgccgct ggcaccacca gcaaacatcg ccattttctg
    25081 tttcacttca atcggcagcc aggcttcata catccagtgc ggcgtcagca taccgaagaa
    25141 gtgaccaaca aaaatcccca gaataccgat atggaacagg ttcgacgcca ggttcatccc
    25201 tttgcgatcc agcatctggc tggacgccgc gcgccaggtg tactgcccgt agtcataacg
    25261 cagccagcta ccaatcagga agaccgcccc ggctatgtac gggtagatat caaagaagaa
    25321 catattcagg aattgcatta gtgctgtcct ccggtggtga tattcagata ttgcggcgcg
    25381 acggcaccgg caaagcgacg ctgatgagca gtgattgctg aatcaccgca gcctttgtca
    25441 gcaaagaatt taacctgctc ttcttcccag acagcatcca gcgcctgcgg cgtatcatcg
    25501 cgcgcttcgt cggcaatttt ttccgccact ttgtcgctgt cgatagcggt attggccaat
    25561 ttcagcagca gatcaaacat cacggcataa cggctttcac gctgttgcag acgcgcgctc
    25621 agcaatgcca gaatcggcgc gatatctttc aaaccttcca cggcttcgct ttgcggcagc
    25681 tgcgacaggt actccagata cagcggcaga tggtccggta attcgcggct gtttaactgc
    25741 aagccgtgct gctcgtactg cgccagcagg tccaccatcg cctggccgcg gtcacgggat
    25801 tcaccgtgaa catgttcaaa tagcaacagt gacgtggcgc ggccacggtc gaacagttcg
    25861 ctgtactggg cctgggcatc gagcggatcc atcgccgtta aatcgcgcag gaaaatgccc
    25921 agcgcatggg catcttcttt cgacagattt ttcgacgcag caatcgcctc aaacatctct
    25981 tgttgatgct gccataaggc agcatccgga tattcaagga gacgcgatac aatcacgagt
    26041 tcgatcatgg atgcggctcc gttttgctgg tcacatcgat ggcatcgata cgacggctgt
    26101 tgaacagatt gaatttggta tctgaaccgt ggcagccatc accaaaggta aagccgcagc
    26161 catttttctc cgggaaggct tcccgtgcca gttcacgatg actactcggc accacaaagc
    26221 gatcttcgta gttagcaatc gccagataac ggtacatctc ctgtgcctgg gcttcggtca
    26281 gaccgacctc ttccagcgca cgggtatcaa ctttaccgtc aacggtttca gcacgtttgt
    26341 aatgacgcat cgccagcata cgtttcagtg cgcgcagtac cggtttggta tcaccggcgg
    26401 tcagcagatt cgccagatac tgtaccggaa tacgcaagct ttcgacgtct ggcagaatgc
    26461 cgttgctacc cagctcaccc gcgtctgctg cagactgaat cggagacaga ggcggcacgt
    26521 accagaccat cggcagtgtg cgatattctg gatgcagcgg cagcgccagt ttccattcca
    26581 ttgccatttt ataaaccggc gactgctgtg cggcttcaat aacgctcagc ggaataccgt
    26641 ctttaatcgc ctgctcgatg actttcggat cgttcggatc gaggaacacc tccagctgac
    26701 gctggtaaag atctttctcg ttctcggtgc tggctgcacg ttcaatcgcg tcggcatcgt
    26761 acagcagcac gccaagataa cggatacgac cgacacaggt ttctgagcac acggtcggct
    26821 gacccgcttc aatacgcgga tagcagaaga tgcacttctc agacttaccg ctcttccagt
    26881 tgaagtagat ttttttgtac gggcatccgg tgatgcacat acgccagcca cggcatttat
    26941 cctggtcgat caggacgatg ccatcttctt cacgcttgta aatcgcaccg ctcgggcagg
    27001 tcgccacaca tgccgggttc aggcagtgtt cgcacaggcg cggcaaatac atcataaagg
    27061 tgttttcgaa ctggctatac atcgccttct ggatgttgtc gaagttcttg tctttcgcca
    27121 gtttgtcaaa ctcaccaccc aggtcatctt cccagttcgg ccctttttcg attttcgcca
    27181 tccgttcccc ggtaatcagc gaacgcggac gggcaatcgg ctgcgatttg ctgccttccg
    27241 gcgcggtatg caggttctga tagtcaaaat cgaacggctc gtaataatcg tcgatccccg
    27301 gcagatgcgg gttagcgaag attttaccca gcaacatggc acggttaccc atgcgcggct
    27361 gcagtttgcc gttgatttta cggatccagc cgcctttgta tttttcctgg ttttcccagt
    27421 cagtcgggaa gccctgaccc ggcttggttt ccacgttgtt gaaccacgcg tattccacgc
    27481 cttcacggct ggtccagacg tttttacagg taactgaaca ggtgtggcag ccgatgcact
    27541 tatcgagatt cagcaccatg ccgacttgtg aacgaatttt cattttacgc tctcctgtac
    27601 ctggtcattg ccttcgccat ctaaccagtc aatgttcttc atcttacgca ctacaacaaa
    27661 ctcatcgcgg ttagaaccta cggtgccgta gtagttaaag ccgtatgcca gatgggcata
    27721 gccgccgatc atatgcgtcg gtttcggcgt gatacgggtg accgagttat ggataccacc
    27781 acgctgttgg gtaatttccg aaccaggcag gttaacgata cgttcctgcg cgtggtacat
    27841 catggtcatc cctgccggaa cacgctggct gacaaccgca cgggcagtca gagcaccgtt
    27901 gctgttgaag acttcaatcc agtcgttatc ggcgataccc agatctttgg catcggcttc
    27961 actcaaccag accaccggac caccgcgacc taaagtcagc atcagcaggt tgtcgctgta
    28021 ggtggagtgg ataccccact tctggtgcgg cgtcaggaag ttgagcgctt tttccgggtt
    28081 gccgttggat ttctggccca tcacttcttt caccgaacgg gtgtcgatcg gcggacgata
    28141 aaccagcagg ctttcaccga aatcacgcat ccattggtga tcctgataca gctgctgacg
    28201 gccagagagc gtacgccacg ggatcagctc gtgaacgttg gtgtaaccgg cgttgtaaga
    28261 aacgtgttca tcttcaaggc cagaccaggt cgggctggag atgattttgc gcggctgtgc
    28321 ctgaatatcg cggaagcgaa tcttctcgtc ttctttattc agcgccagat gcgtatggtc
    28381 acgaccggta aactcgctca gggcagccca ggctttcacg gctacctgac cgttggtttc
    28441 cggtgccagc gtcaggatca tctctgccgc atcaattgcg gtgttcagca tcggctggcc
    28501 tttcgccgga ccttccgctt tggtgtagtt gagcttacgc agcagatcca tctcgctctg
    28561 ggtgttccag gcaatccctt taccgccatt accgattttc tccatcagcg ggccgataga
    28621 ggtaaagcgt tcgtaagtcg ccggataatc gcgctctacg accataatgt gtggcgcggt
    28681 tttaccaggg atcaggtcgc actcgccttt tttccagtct ttcacatcca gcggctgcgc
    28741 cagttcagcg gcagagtcgt gctggatagg cagcgtgacg atgtcggttt ctttacccag
    28801 atgaccgacg cacacttcgg agaatttctt cgcgatggct ttgtaaattt cccagtcgct
    28861 tttcgcttcc caggccggat cgaccgccgc agacagcggg tgaataaacg gatgcatatc
    28921 cgaagtattc atgtcgtctt tttcgtacca ggtcgccgtc ggcaaaatga tgtcggaata
    28981 gagacaggtg ctcgacagac ggaagtccag cgtaaccacc agatccagct tgccttccag
    29041 accattgtcc tgccagtcca cttcttccgg cttcacgccg ccctgttgcc ccagatcttt
    29101 accctggata ccgtgctccg tccccagcag gtacttgagc ataaactcat gacctttacc
    29161 ggaagaaccg agcaggttag aacgccagat gaacaggttg cgcgggtggt ttttaccgtt
    29221 ttctggttgt tctgccgcaa aacggatgga accctctttc agggatttca ccgtatagtc
    29281 caccggattc atcccggctt tttccgcttc gccagcgata gtcagcgggt tagtgcctaa
    29341 ctgcggtgca gacggcagcc agcccatgcg ttccgcacgg acgttaaagt cgatcaagtg
    29401 tccggtatag cgggatttgt ccgtcatcgg tgacagcaac tcttccgccg tgacggtttc
    29461 ataacgccac tggctggagt ggttatagaa ataagaagtg ctgttcatgt gacgcgccgg
    29521 acgctgccag tcaagggcaa acgccagcgg ctgccagccg gtttgcggac gcagtttttc
    29581 ctgacctaca tagtgcgccc agccgccccc gctctgaccg acacagccgc agaaaatcag
    29641 catgttgatc agaccacgat agttcatatc gaggtgatac cagtggttca gccccgcacc
    29701 gacgataatc atcgaacgac cgtgcgtttt atcagcgtta tcggcaaatt cacgggcgat
    29761 gcgaataatc tggctgcgag aaacgccggt aatctgctcg gcccaggccg gggtataagc
    29821 tttcacatcg tcatagctgg ttgcacagtt aacgtcgttc aggccacgtt ccagaccgta
    29881 gtttgccagc gtcagatcat aaacggtggt caccagggcg gtgctgccat cagccagttg
    29941 caggcgtttc accggcagtt tgtgcagcag cacgttttcc agttccactt tgttgaagtg
    30001 ttccgtgccg tcgccaccaa agtacgggaa acctacctca gcgatctcat cctggctacc
    30061 cagcaggctc agttgcagct cggtttcttc gccagttttg ccgtcgcgct gctcaagatt
    30121 ccatttgccc ttctcgcccc agcggaagcc aatagaaccg ttcggcgcaa ccatttcgcc
    30181 attggtatta aaggcgacag ttttccattc cggattgttt tcctggccca gcgcatcaac
    30241 cagatcagca gcgcgcagca tacgacctgc agcgtagtaa ccgtcgcgtt cttccagcat
    30301 caccagcatc ggcatgtcgg tgtagcgacg cacatagtcg gtgaaatact ggcttgggtt
    30361 gtcgaggtgg aattcacgca gcattacgtg gcccatcgcc agcgccattg ccgcatcggt
    30421 gccctgtttc ggtgccagcc acagatcgca cagtttggcg atttcagcgt agtctggtgt
    30481 gacggcaaca gttttggtcc ctttgtaacg cacttcagta aagaagtgag catccggggt
    30541 acgcgtctgc ggtacgtttg acccccaggc gatgatgtag ctggagttgt accagtcagc
    30601 agattccggt acgtcagttt gctcgcccca ggtttgcgga gacgcaggag gcaagtcgca
    30661 gtaccagtcg tagaagctta agcaagtacc gccaatcagc gagagatagc gtgcacccga
    30721 tgcgtaagaa accatcgaca ttgccggaat tggcgagaaa ccagcaacac ggtccgggcc
    30781 gtagtttttg atggtgtaaa cgttagatgc ggcgatcagt tcgttcacct cctgccagga
    30841 agaacgaaca aatccaccgc gtccacgcgc ctgcttaaag cttttcgctt tatcggcgtc
    30901 ttcaatgata gaagcccatg cctcaaccgg atcgctatgc agcgccttcg cttcacgcca
    30961 cattttcatc aggcgtttgc gcatcatcgg gtatttcagg cggttggcac tgtaaagata
    31021 ccaggagtag ctggcaccgc gcgggcagcc gcgaggttca tggtttggca gatccggacg
    31081 ggtacgcgga tagtcagtct gctgggtttc ccaggtgacc agaccgtttt tgacgtagat
    31141 tttccagctg caggagccgg tgcagtttac cccgtgggta gagcggacga ttttgtcatg
    31201 ctgccaacgc tggcgatatc catcctccca gtcacggttg gtattgagaa gctggccatg
    31261 cccatcggca aaggtttcac ccttctgttt gaagtagcga aaccggtcca ggaatttact
    31321 catcggtttt ctcctgtggg agcctgtcgg ccctctgata aatcgacatt gctaatgttt
    31381 aagggcaagg taacgctctg aaacagcgtg ggaattgata acgatcaagt gtgatgggga
    31441 tgaaaaataa agtaaattcc taagataccc ctttaggata attaacagtt aaagcataac
    31501 atgctgtaaa ttaagacgtt ttatacctgt agttatttaa agc
//
LOCUS       DAZGXZ010000052        30997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000052 DAZGXZ010000000
VERSION     DAZGXZ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.052"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKJF_002452"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKJF_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP0949794.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHKJF_002453"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHKJF_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP0949795.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHKJF_002454"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHKJF_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP0949796.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKJF_002455"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHKJF_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP0949797.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKJF_002456"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHKJF_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP0949798.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKJF_002457"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHKJF_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP0949799.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKJF_002458"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHKJF_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP0949800.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKJF_002459"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHKJF_002459"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP0949801.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKJF_002460"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHKJF_002460"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP0949802.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKJF_002461"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHKJF_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFP0949803.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKJF_002462"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHKJF_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP0949804.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKJF_002463"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHKJF_002463"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP0949805.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASDSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKJF_002464"
                     /pseudo
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHKJF_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKJF_002465"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHKJF_002465"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP0949806.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKJF_002466"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHKJF_002466"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP0949807.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKJF_002467"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHKJF_002467"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP0949808.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPITAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKJF_002468"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHKJF_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP0949809.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKJF_002469"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHKJF_002469"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP0949810.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKJF_002470"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHKJF_002470"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP0949811.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKJF_002471"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHKJF_002471"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP0949812.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKJF_002472"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHKJF_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP0949813.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKJF_002473"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHKJF_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP0949814.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKJF_002474"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHKJF_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP0949815.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKJF_002475"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHKJF_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP0949816.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKJF_002476"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHKJF_002476"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP0949817.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALRHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKCSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKJF_002477"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHKJF_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP0949818.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNIALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIAGTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKJF_002478"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHKJF_002478"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP0949819.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNIYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKJF_002479"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHKJF_002479"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP0949820.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7302 a         8032 c         8480 g         7183 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagacccc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gtatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaatg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctaggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtacagcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gcacgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acagaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt tcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gagttgcgag gcatttagcg
    14881 cccgttctac gtcagcagcc gttttgccgt tgatacgcag gcggatgtcg tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg cgacggcaat
    15121 tgccgtattc actaacaccg cgtcggcccc catttccagc gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg cactgtggcc tgctggataa taatctccag
    15241 catggcgcgg gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcca gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcgtttc ggcggctttc agggtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc taattttaac cagtttgtgc ctaacgcttc
    15481 acgagccaga tgggcggcga aaatggcttc ttccgctgtt ttcgccccgg atgtatttgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgtcg gaagcgcgga tcgcctccac
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacattt caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gcccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt actccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtgcc
    16261 acgcaggcgg cattaatctc ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggctg
    16441 ttgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcca ggtacagcgc agcaggtgta
    16561 cccagcccgc ccagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaagtcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cagcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgata ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaga atttcggccc acacatggag
    17461 caaaaatggg cgactttacc tgactcttgc ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggttatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacctcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggcgtga tgatgccctg gcgggcgtag tgcagttggg tgacacggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagaatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccgctg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgagct
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcgacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 gctcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggcagtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctc tattacatgc ataaaattgt tgatggttta gtgcgtgaaa acgacggtcg
    21541 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21661 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21721 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagtga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaagat ccaccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atatccctcg cttgagctgg tggagtataa
    22081 agttgcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctg ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gcttattggt
    22261 ggatgtgccg accattggcg tggcgaaaaa acgtctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccgctgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgagagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggctggc gctctgcttc tcactgcctg
    23761 tagtcataac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaatatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctga tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctgattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatctcg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaatgagtaa aaatgactcg
    24901 cctgctgcgg gtagcgagtt atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtgg ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 cgcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattattc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagaag ctggtggcgc
    25741 tgaggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagttg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aagccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaatgctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacatcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 ggagatggac ggcatcgcca cgctgaaaga gatcaaagcg ttaaacccgg caattccggt
    26641 gctgattatg actgcgtact ccagcgtcga gacggcggta gaggcactga aaactggggc
    26701 gctggattat ctcatcaagc cgctggattt cgataaccta caggcgacgc tggaaaaggc
    26761 gctcgcgcat acgcacagta ttgatgctga aacacctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acagtgctga tccacggcga ttccggcacc ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgcggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aggatatccc
    27361 tctgctggct ggccattttc ttcagcgttt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ctttacgcca caggcgatgg atttgctgat ccactacgac tggccgggaa acattcgtga
    27481 actggaaaac gcggtggaaa ggtcggtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccggtacgcc gatcccgctg gtacaaagtc aggatattca
    27601 gccgttggtg gaagtggaaa aagaggtgat tctggcggca ctggagaaaa cgggcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgttatccgc
    27901 cagtttcgtg cccgcgtgga acactttgcc gtcagccact tcttccagcg gcaggccgtg
    27961 gatcacatcg ccggtgcggt aatcgcccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgc tcatcccact cggacgtttt ctcgtccagc ttgccttcac aggccgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ctggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 atagagaaaa ccggtgtaga tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacgtc atcggttact accggcgcgg gggagtaagc
    28321 tcccatcccg ccggtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcagcacat gctcgccgtc caccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagt gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaatca ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcgg cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatctagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccagc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccgcattgt
    30721 aacgaaaacg tttgcgcagc gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc ctccatcgac acggcggatg
    30961 tgaatcactt cacacaaaca gccggttcgg ttgaaga
//
LOCUS       DAZGXZ010000053        30318 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000053 DAZGXZ010000000
VERSION     DAZGXZ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.053"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(32..853)
                     /gene="bacA"
                     /locus_tag="ACHKJF_002480"
     CDS             complement(32..853)
                     /gene="bacA"
                     /locus_tag="ACHKJF_002480"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0949821.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHETIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(943..1311)
                     /gene="folB"
                     /locus_tag="ACHKJF_002481"
     CDS             complement(943..1311)
                     /gene="folB"
                     /locus_tag="ACHKJF_002481"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP0949822.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            1416..2033
                     /gene="plsY"
                     /locus_tag="ACHKJF_002482"
     CDS             1416..2033
                     /gene="plsY"
                     /locus_tag="ACHKJF_002482"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP0949823.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(2046..2978)
                     /gene="ttdR"
                     /locus_tag="ACHKJF_002483"
                     /gene_synonym="dan"
     CDS             complement(2046..2978)
                     /gene="ttdR"
                     /locus_tag="ACHKJF_002483"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP0949824.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            3185..4096
                     /gene="ttdA"
                     /locus_tag="ACHKJF_002484"
     CDS             3185..4096
                     /gene="ttdA"
                     /locus_tag="ACHKJF_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP0949825.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            4093..4698
                     /gene="ttdB"
                     /locus_tag="ACHKJF_002485"
     CDS             4093..4698
                     /gene="ttdB"
                     /locus_tag="ACHKJF_002485"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP0949826.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            4747..6210
                     /gene="ttdT"
                     /locus_tag="ACHKJF_002486"
     CDS             4747..6210
                     /gene="ttdT"
                     /locus_tag="ACHKJF_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP0949827.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLESILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(6253..7266)
                     /gene="tsaD"
                     /locus_tag="ACHKJF_002487"
     CDS             complement(6253..7266)
                     /gene="tsaD"
                     /locus_tag="ACHKJF_002487"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP0949828.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            7504..7719
                     /gene="rpsU"
                     /locus_tag="ACHKJF_002488"
     CDS             7504..7719
                     /gene="rpsU"
                     /locus_tag="ACHKJF_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP0949829.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            7830..9575
                     /gene="dnaG"
                     /locus_tag="ACHKJF_002489"
     CDS             7830..9575
                     /gene="dnaG"
                     /locus_tag="ACHKJF_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0949830.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            9770..11611
                     /gene="rpoD"
                     /locus_tag="ACHKJF_002490"
     CDS             9770..11611
                     /gene="rpoD"
                     /locus_tag="ACHKJF_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP0949831.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(11690..12196)
                     /gene="mug"
                     /locus_tag="ACHKJF_002491"
     CDS             complement(11690..12196)
                     /gene="mug"
                     /locus_tag="ACHKJF_002491"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP0949832.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            12321..12396
                     /locus_tag="ACHKJF_002492"
     tRNA            12321..12396
                     /locus_tag="ACHKJF_002492"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12354..12356,aa:Ile,seq:cat)
     gene            complement(12450..13214)
                     /gene="nfeF"
                     /locus_tag="ACHKJF_002493"
                     /gene_synonym="yqjH"
     CDS             complement(12450..13214)
                     /gene="nfeF"
                     /locus_tag="ACHKJF_002493"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP0949833.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            13491..14114
                     /gene="nfeR"
                     /locus_tag="ACHKJF_002494"
                     /gene_synonym="yqjI"
     CDS             13491..14114
                     /gene="nfeR"
                     /locus_tag="ACHKJF_002494"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP0949834.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(14268..15788)
                     /gene="aer"
                     /locus_tag="ACHKJF_002495"
     CDS             complement(14268..15788)
                     /gene="aer"
                     /locus_tag="ACHKJF_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP0949835.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLS
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            16206..17585
                     /gene="ygjG"
                     /locus_tag="ACHKJF_002496"
     CDS             16206..17585
                     /gene="ygjG"
                     /locus_tag="ACHKJF_002496"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP0949836.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(17627..17959)
                     /locus_tag="ACHKJF_002497"
     CDS             complement(17627..17959)
                     /locus_tag="ACHKJF_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP0949837.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            18178..19161
                     /gene="ebgR"
                     /locus_tag="ACHKJF_002498"
     CDS             18178..19161
                     /gene="ebgR"
                     /locus_tag="ACHKJF_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP0949838.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            19345..22437
                     /gene="ebgA"
                     /locus_tag="ACHKJF_002499"
     CDS             19345..22437
                     /gene="ebgA"
                     /locus_tag="ACHKJF_002499"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP0949839.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            22434..22883
                     /locus_tag="ACHKJF_002500"
     CDS             22434..22883
                     /locus_tag="ACHKJF_002500"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP0949840.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            22946..24379
                     /locus_tag="ACHKJF_002501"
     CDS             22946..24379
                     /locus_tag="ACHKJF_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0949841.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            24513..25583
                     /gene="ygjJ"
                     /locus_tag="ACHKJF_002502"
     CDS             24513..25583
                     /gene="ygjJ"
                     /locus_tag="ACHKJF_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP0949842.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            25600..27951
                     /gene="ygjK"
                     /locus_tag="ACHKJF_002503"
     CDS             25600..27951
                     /gene="ygjK"
                     /locus_tag="ACHKJF_002503"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP0949843.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            28277..30295
                     /gene="fadH"
                     /locus_tag="ACHKJF_002504"
     CDS             28277..30295
                     /gene="fadH"
                     /locus_tag="ACHKJF_002504"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP0949844.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPAGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         7329 a         8185 c         7851 g         6953 t
ORIGIN      
        1 aaaccaggcc tgctcccgaa ccttttctca atcaaaagaa cacgacgtac accgcagccg
       61 ccacaataaa gcgataaatg gcgaacggga taaacgaaat gcgcttaatc aattgcagga
      121 aggttttaat cgctatcagc gccaccacaa aagcggtgat aaacccaacg gcaaacatcg
      181 ggatatcgcc ggttgtcagg aagccccagc ttttgtagag atcgagcgcc gttgcgccca
      241 tcatcatcgg taccgccagc aggaacgaga actcggaagc agcgtaacgg ctcacgccca
      301 tcagcatccc acctgaaatg gtggccccgg aacgggagaa acccggccac agcgccagac
      361 actggaaaca gccaatcata aacgcctgac gataggtcat atcatcaaga cccggcgcac
      421 gcggctcttt cggcttcagg cattcggcgg caatcagcaa caaaccgcca acgaccagcg
      481 catacatcac atttatcggg ttaaacaatg acttaatcgt ttcgtggaac aatagcccca
      541 gtaccaccgc cggaatcatc cccagcaaaa tgtggatcag cgttaaacga cctttgcttt
      601 caccttcgtg ctgcaacggg cggccaaagt ggatgccaat caggccaaac agacgccgcc
      661 agaacatcac tactaccgcc agaattgatc ctaactggat cacaacttca aaggttttcg
      721 ccgtgtcgcc ctcaaacccc aacaagtgac cgacaataat catatggccc gtgctggata
      781 ccggcagaaa ttctgtcaat ccttcgacca cacccaatat tgccgctatc agcagcgagt
      841 gcatatcgct catcaataaa cccctaaatt attaaaatgt accgcttgtc cgaactactg
      901 cgtatgacca ggttataacc gtttggttta acagctgtaa aattaattat tttctttcag
      961 attattgcca cgctcaatga ttacgccaac attcgccgcc cgcgccactg cgcctggctt
     1021 gctgagtttg atacgcaccc acggcgagtt gaagcgtgct aacagcagct ccgccacctc
     1081 ttcagccacg cgttccacca gcgcaaaacg cgccccctcg acgtggctga ccaccgtttc
     1141 tgcaatgtca gcgtaactga ggcaatccgc cacatcatca cttttcgccg ctttacggtt
     1201 atcccatgcc atttcgatat cgaacactaa cttctgttcg atggtctgtt cccagtcgta
     1261 aacaccaata gtggtgatta ccgaaagttg ctctataaat acaatatcca tcacgtcctg
     1321 cctgcttttt ggctaaccgg ataccacttc cggcgaaatg tgcgtattat ccacagattc
     1381 atcgttgaac acgaattttc aaaacggaac agcttatgag tgcaatcgcg cctggaatga
     1441 tcctcatcgc gtacctctgc ggctccattt ccagtgccat tctggtttgc cgcttgtgtg
     1501 ggctgcccga tccgcgaacc agcggctccg gcaatccagg cgcaaccaat gtgttacgta
     1561 tcggtggcaa gggagcagcc gtagcagtac tgattttcga cgttctgaaa ggaatgttgc
     1621 ccgtctgggg cgcgtatgaa ttaggtgtca gccccttctg gctaggctta attgccatcg
     1681 ccgcctgtct tggacacatc tggcccgttt tcttcggatt taaaggagga aaaggcgttg
     1741 ctaccgcttt tggtgccatc gcacccattg gctgggatct caccggagta atggcgggaa
     1801 cctggttact gaccgtgcta ttgagcggat actcgtcgct gggagcgatt gtcagtgcac
     1861 tgattgctcc gttttatgta tggtggttta agccacaatt caccttcccg gtttcgatgc
     1921 tctcttgcct gatcctgctg cgtcatcatg acaacatcca gcgtctgtgg cgtcgtcagg
     1981 agacaaaaat ctggacgaaa ttcaaaagaa agcgcgaaaa ggatcccgag tgatttctgg
     2041 tggatctaca tgacctgata gccttcatcg ggcttgccca gccgttgctg gcaccatgcc
     2101 gccagaaatt ccacgcagac acgtaatttc atgctgcgat agagcggctc ccggtaaaca
     2161 gcccagatat tggcgctttg tgcatactcc ggcaatactt gcaccagttt gccactctcc
     2221 agaaacggca acacatccca ctcggaacgc agcataatcc ctttgccctc cagcgcccat
     2281 tgcaacacta tctcgccgct attggaggaa agatgcccgc taaccttcac cgattttttc
     2341 tcctgaccgt tccccaactc ccatattccg tgggtcatat cgcgctcttt ggtcaccaga
     2401 cagtcatgac gacttaattc ctgtaatgat tgtggctgcg gatatttttg cagatactca
     2461 ggtgctgcgc ataatattct tttatttttc gttaacagat gcgcaatata ataatcagga
     2521 atttcgtcat taatacgaat atccagatca atattatcct gcactaaatc aatttgccga
     2581 tcgaacaatt caaaatgcac ctgtaactca ggataattgc gcataagttc ggtaatggct
     2641 ggcgcaatat ggctgcgccc aaaaccaaaa ctgcaaccaa tacgaatcat cccctccggg
     2701 cgcgttttga tttgcgtgac gtcatcgacc agtcgctgat attgggtgag gatttccagc
     2761 gcatgttcgt aacaacgttg gccactttca gtcagcgcca cgccgcgagc ggagcggttc
     2821 agcagcgttg ttgccagcgt attttcaagg atttggatcc gcttggtgac aaatgcaggc
     2881 gtttgcccaa gcgtcgccgc tgcagcacta aaactcccgc tgtggacgat ttctaccagt
     2941 acctgcaagt ctttggctaa aggccagcta ttcagcatcg ttaattatcc gcagttgtga
     3001 taagcgcagt gtatttcgca aaacattgcc attccctgct ctggcgcaat attcattctt
     3061 attttcgcgt tgattcacga actgttaatt acatattcac ggtggcaaaa aatataaaac
     3121 cacattttta gtggtagttt gtggcggtga atttttccag acaaatacaa aaactggagt
     3181 tgccatgatg agcgaaagta ataagcaaca ggcagtgaat aagttgacag agattgtcgc
     3241 taactttacc gccatgattt ctacccgaat gcctgatgac gtggtggata aactaaaaca
     3301 gctaaaggat gccgaaacgt cgtcgatggg gaaaattatc taccatacga tgttcgacaa
     3361 catgcaaaaa gcgattgacc tgaatcgtcc tgcctgtcag gacaccgggg agattatgtt
     3421 cttcgttaaa gtcggttccc gcttcccact gcttggcgag ctgcaaagca tactcaaaca
     3481 agccgtggaa gaggcaaccg tcaaagcgcc actacgtcac aatgcggtag aaatttttga
     3541 cgaagtaaac accggcaaaa ataccggtag cggcgtaccg tgggtcacct gggacatcat
     3601 ccccgacaat gacgatgcag aaatcgaagt ttacatggca ggcggcggct gcacgctacc
     3661 tggccgctcg aaagtgttaa tgccgtcaga aggctacgaa ggcgtggtga aattcgtctt
     3721 cgaaaatatc tccaccctcg ccgtaaacgc ctgtccaccg gtactggtgg gcgtgggcat
     3781 cgccacctcg gtggaaaccg ccgccgtact ctcgcgtaaa gccattttgc gtccgattgg
     3841 cagccgccat cccaatccaa aagcggcaga gctggagcta cgcctggaag aaggactcaa
     3901 ccgtctgggg attggtccac aagggctgac cggcaacagt tcagtgatgg gcgtgcatat
     3961 cgaatctgcc gcccgccatc cgtcaaccat cggcgttgct gtctctaccg gctgctgggc
     4021 gcatcgtcgc ggcacgctgc tggttcatgc cgatctcacc tttgaaaatc tgtctcacac
     4081 ccggagcgcg ttatgaaaaa gatcctgaca accccgatca aagctgaaga tctgcaagat
     4141 attcgcgtcg gcgatgtgat ctacctgacc ggtacgctgg tgacctgccg tgacgtttgt
     4201 caccgccgct tgatcgaact gaaacgtccg atcccttacg atctcaacgg caaagcgatt
     4261 ttccacgctg gccccatcgt gcgcaaaaac ggcgataaat gggagatggt ttccgttggc
     4321 ccgacaacca gtatgcgtat ggaaagtttt gaacgtgaat ttatcgagca gaccggcgtg
     4381 aaactggtgg ttggcaaagg cggaatgggg ccgctgaccg aagaaggctg ccagaaattc
     4441 aaggcgctac atgtgatttt cccggcaggc tgcgcggtgc tggcggcaac ccaggtggaa
     4501 gagattgaag aagtgcactg gacagaactc gggatgccgg agtcactgtg ggtctgccgg
     4561 gtcaaagagt tcggcccgct gattgtctct attgataccc acggcaacaa cctgatagcc
     4621 gaaaacaaaa agctgttcgc cgaacgccgc gatcccatcg tggaagagat ctgcgagcac
     4681 gtccattaca tcaaataacc ctcccggaga ggctcgcccc ctctcctttt tcgcaggcat
     4741 aacacgatga aaccttccac tgaatggtgg cgataccttg cgccgctggc ggtcatcgcc
     4801 attattgctc taattccggt tcccgcaggg ctggagagtc atacctggct ctactttgcc
     4861 gtttttactg gcgtgatcgt tggactgatc ctcgaacccg tgccgggtgc cgtggtggcg
     4921 atggtgggta tctccatcat cgccatactc tctccctggc tgctgttcag ccccgaacag
     4981 ctcgcccagc caggctttaa attcactgca aaatccctct cgtgggcggt ttccggtttt
     5041 tctaattcgg ttatctggct gattttcgcc gcctttatgt ttggcacagg ctatgaaaaa
     5101 accgggctgg gacgccgcat tgcgctgatt ctggtgaaaa agatgggaca tcgcacgctg
     5161 tttctcggct atgcggtgat gttctccgag ctgatcctcg cacctgtaac accgtccaac
     5221 tcggcgcgtg gtgcggggat tatctatccc atcatccgta acctgccacc gctctatcaa
     5281 tcacaaccaa acgacagcag ttcgcgcagc attggctcgt acatcatgtg gatggggatt
     5341 gttgccgact gcgtgaccag cgccattttc ttgacggcga tggcaccaaa cttgctgtta
     5401 attggactga tgaaaagcgc atctcacgcc acgctgagtt ggggcgactg gttcctcggg
     5461 atgttgccgc tcagtatttt gctggtgctg ctggttccct ggctggctta tgtgctgtac
     5521 ccgccggtac tgaagtcagg cgatcaggtg ccgcgctggg cagagacgga actgcaggca
     5581 atgggcccgc tctgttcgcg tgaaaaacgg atgctggggc tgatggtagg cgcgctggtg
     5641 ctgtggattt tcggcggtga ttatatcgat gccgcgatgg tcggttacag cgtggtggca
     5701 ctgatgctgc ttctgcgcat tatcagttgg gacgacattg tcagtaataa agccgcgtgg
     5761 aacgttttct tctggctggc ctcgcttatc accctcgcta ccggactcaa caacactggt
     5821 tttattagct ggtttggcaa actgttagca ggcagcttaa gcggttattc gccaacgatg
     5881 gtgatggtgg cgttgattgt ggtgttttat ctgctgcgct actttttcgc cagcgccacg
     5941 gcgtatacct ccgccctcgc accgatgatg attgccgctg cgctggcgat gccggaaatc
     6001 ccgctgccgg tgttctgcct gatggttggc gcggcaattg gtctggagag cattcttaca
     6061 ccatacgcca ccggacccag tccgatttac tacggtagtg gttatctgcc aacggtggat
     6121 tactggcgac tgggggcgat ttttggactg atattcctcg tgttgctggt gattaccggc
     6181 ttgctgtgga tgcccgtggt gttgctttaa gtgaggacgg tgccggaaat tgctctccgg
     6241 caccggatag ttttacgcag ccggtaactc tgccagtggc cagcgcggac gcacgctaac
     6301 gccgagatcc gccgtcgcgc ctgctttaaa ccgcaccatt ccggcatagg cgatcatcgc
     6361 gccgttatca gtacaaaatt ccggacgcgc gtagaacact tcgccgcggc gttttttcat
     6421 catttccgcc agcttcgccc gtaacgtgcg gttagcactc acgccgcccg ccatgaccag
     6481 tcgcttaaag cccgtctgat ccaacgctcg cttacactta atcatcaacg tatcgaccac
     6541 cgcatcttca aaggcgcggg cgatatcagc acgcgtctgg tcgtcggtgc cgttgtcacg
     6601 aatcgtgttc gccgcaaagg ttttcagacc agaaaagctg aaatccagcc ccggacggtc
     6661 ggtcatcgga cgcgggaaaa caaagcgccc ggcagtaccc tgagccgcca ttttcgacag
     6721 taacggtccg ccaggataat ccagccccag cagcttcgcg gttttatcaa acgcttcacc
     6781 ggcggcatca tcgatagact cgccgagcag ctcgtactga ccaatgccag tcacgctgat
     6841 taactgcgta tgaccgccgg acaccagcag cgcgacaaac ggaaattccg gcgggttatc
     6901 ttccagcatc ggcgctaaca gatgcccttc catatggtgt accgggattg ccggaacgtt
     6961 ccaggcaaac gccagagaac gccccacggt tgcgccaacc agcagtgcgc cgactaatcc
     7021 agggcctgcg gtataggcca cagcatcaat gtcttttgcc gttaaaccag actcctttag
     7081 cgccgcctgg atcaacggta cggttttgcg cacgtgatcg cgggaggcca gttcaggcac
     7141 gacgccgccg tagtcagcgt gcaatttcac ctgactatac aattggttgg ctaacaaacc
     7201 tttttcatcg tcgtaaatgg cgatgccggt ttcatcgcag gaagtttcaa tacccagtac
     7261 acgcatgact tgttttacct cgctttatta ccgcgcagtg taggaccaat gcgggttgat
     7321 gtaaaacttt gttcgcccct ggagaaagcc tcgtgtatac tcctcaccct tataaaagtc
     7381 cctttcaaaa aaggccgcgg tgctttacaa agcagcagca attgcagtaa aattccgcac
     7441 cattttgaaa taagctggcg ttgatgccag cggcaaaccg aattaatcaa aggtgagagg
     7501 cacatgccgg taattaaagt acgtgaaaac gagccgttcg acgtagctct gcgtcgcttc
     7561 aagcgttcct gcgaaaaagc aggtgttctg gcggaagttc gtcgtcgtga gttctatgaa
     7621 aaaccgacta ccgaacgtaa gcgcgctaaa gcttctgcag tgaaacgtca cgcgaagaaa
     7681 ctggctcgcg aaaacgcacg ccgcactcgt ctgtactaat tccccgagag cgttgctctc
     7741 cgatcagacc gagttgtagt tgtaaggccg tgcttccgaa aggaatgcgc ggcttatttt
     7801 cgtttatgaa ttgctaaaaa tcggggccta tggctggacg aatcccacgc gtattcatta
     7861 atgatctgct ggcacgcact gacatcgtcg atctgatcga tgcccgtgtg aagctgaaaa
     7921 agcagggcaa gaatttccac gcgtgttgtc cattccacaa cgagaaaacc ccgtcattca
     7981 ccgttaacgg tgagaaacag ttttaccact gctttggatg tggcgcgcac ggcaacgcga
     8041 tcgacttcct gatgaactac gacaagctcg agttcgttga aacggtcgaa gagctggcag
     8101 caatgcacaa tcttgaagtg ccatttgaag caggcagcgg ccccagccag atcgagcgcc
     8161 atcagaggca aacgctttat cagttgatgg acggtctgaa tacgttttac caacaatctt
     8221 tacaacaacc tgttgccacg tctgcgcgcc agtatctgga aaaacgcgga ttaagccacg
     8281 aggttatcgc tcgctttgcg attggttttg cgccccccgg ctgggacaac gtcctgaagc
     8341 ggtttggcgg caatccagaa aatcgccagt cattgattga tgcggggatg ttggtcacta
     8401 acgatcaggg acgcagttac gatcgtttcc gcgagcgggt gatgttcccc attcgcgata
     8461 aacgcggtcg ggtgattggt tttggcgggc gcgtgctggg caacgatacc cccaaatacc
     8521 tgaactcgcc ggaaacagac attttccata aaggccgcca gctttacggt ctttatgaag
     8581 cgcagcagga taacgctgaa cccaatcgtc tgcttgtggt cgaaggctat atggacgtgg
     8641 tggcgctggc gcaatacggc attaattacg ccgttgcgtc gttaggtacg tcaaccaccg
     8701 ccgatcacat acaactgttg ttccgcgcga ccaacaatgt catttgctgt tatgacggcg
     8761 accgtgcagg ccgcgatgcc gcatggcgag cgctggaaac ggcgctgcct tacatgacag
     8821 acggccgtca gctacgcttt atgtttttgc cggatggcga agaccctgac acgctggtac
     8881 gaaaagaagg taaagaagcg tttgaagcgc ggatggagca ggcgatgccg ctttccgcat
     8941 ttctgtttaa cagcctgatg ccgcaagttg atctgagtac ccctgacggg cgcgcacgtt
     9001 tgagtacgct ggcactgcca ttgatatcgc aagtgccggg cgaaacgctg cgaatatatc
     9061 ttcgtcagga attaggcaac aaattaggca tacttgatga cagccagctt gaacgattaa
     9121 tgccaaaagc ggcagagagc ggcgtttctc gccctgttcc gcagctaaaa cgcacgacca
     9181 tgcgtatact tatagggttg ctggtgcaaa atccagaatt agcgacgttg gtcccgccgc
     9241 ttgagaatct ggatgaaaat aagctccctg gacttggctt attcagagaa ctggtcaaca
     9301 cttgtctctc ccagccaggt ctgaccaccg ggcaactttt agagcactat cgtggtacaa
     9361 ataatgctgc cacccttgaa aaactgtcga tgtgggacga tatagcagat aagaatattg
     9421 ctgagcaaac cttcaccgac tcactcaacc atatgtttga ttcgctgctt gaactgcgcc
     9481 aggaagagtt aatcgctcgt gagcgcacgc atggtttaag caacgaagaa cgcctggagc
     9541 tctggacatt aaaccaggag ctggcgaaaa agtgatttaa cggcttaagt gccgaagagc
     9601 gatcgggaag cccccgacag ccgcactgag aggcagcggc aaatatataa gtacgccctc
     9661 gtaattatcg ttggcggtaa acaaccgttg gatttcagcg ttaacggctg aaggacatcg
     9721 ggtcaatcgc ccaacaccaa cctcatgaaa taagtgtgga taccgtctta tggagcaaaa
     9781 cccgcagtca cagctgaaac ttcttgtcac ccgtggtaag gagcaaggct atctgaccta
     9841 tgccgaggtc aatgaccatc tgccggaaga tatcgtcgat tcagatcaga tcgaagacat
     9901 catccaaatg atcaacgaca tgggcattca ggtgatggaa gaagcaccgg atgccgatga
     9961 tctgatgctg gctgaaaaca ccgcggacga agatgctgcc gaagccgccg cgcaggtgct
    10021 ttccagcgtg gaatctgaaa tcgggcgcac gactgacccg gtacgcatgt acatgcgtga
    10081 aatgggcacc gttgaactgt tgacccgcga aggcgaaatt gacatcgcta agcgtattga
    10141 agacgggatc aaccaggttc aatgctccgt tgctgaatat ccggaagcga tcacctatct
    10201 gctggaacag tacgatcgtg ttgaagcaga agaagcgcgt ctgtccgatc tgatcaccgg
    10261 ctttgttgac ccgaacgcag aagaagatct ggcacctacc gccactcacg tcggttctga
    10321 gctttcccag gaagatctgg acgatgacga agatgaagac gaagaagatg gcgatgacga
    10381 cagcgccgat gatgacaaca gcatcgaccc ggaactggct cgcgaaaaat ttgcggaact
    10441 acgcgctcag tacgttgtaa cgcgtgacac catcaaagcg aaaggtcgca gtcacgctgc
    10501 cgctcaggaa gagatcctga aactgtctga agtattcaaa cagttccgcc tggtgccgaa
    10561 gcagtttgac tacctggtca acagcatgcg cgtcatgatg gaccgcgttc gtacgcaaga
    10621 acgtctgatc atgaagctct gcgttgagca gtgcaaaatg ccgaagaaaa acttcattac
    10681 cctgtttacc ggcaacgaaa ccagcgatac ctggttcaac gcggcaattg cgatgaacaa
    10741 gccgtggtcg gaaaaactgc acgatgtctc tgaagaagtg catcgcgccc tgcagaaact
    10801 gcagcagatt gaagaagaaa ccggcctgac catcgagcag gttaaagata tcaaccgtcg
    10861 tatgtccatc ggtgaagcga aagcccgccg tgcgaagaaa gagatggttg aagcgaactt
    10921 acgtctggtt atttctatcg ccaagaaata caccaaccgt ggcttgcagt tccttgacct
    10981 gattcaggaa ggcaacatcg gtctgatgaa agcggttgat aaattcgaat accgccgtgg
    11041 ttacaagttc tccacctacg caacctggtg gatccgtcag gcgatcaccc gttctatcgc
    11101 ggatcaggcg cgcaccatcc gtattccggt acatatgatt gagactatca acaaactcaa
    11161 ccgtatttct cgccagatgc tgcaagagat gggccgtgaa ccgacgccgg aagaactggc
    11221 tgaacgtatg ctgatgccgg aagacaagat ccgcaaagtg ctgaagatcg ccaaagagcc
    11281 aatctccatg gaaacgccga tcggtgatga tgaagattcg catctggggg atttcatcga
    11341 ggataccacc ctcgagctgc cgctggattc tgcgaccacc gaaagcctgc gtgcggcaac
    11401 gcacgacgtg ctggctggcc tgaccgcgcg tgaagcgaaa gttctgcgta tgcgtttcgg
    11461 tatcgatatg aacaccgacc acacgctgga agaagtgggt aaacagttcg acgttacccg
    11521 cgaacgtatc cgtcagatcg aagcgaaggc gctgcgcaaa ctgcgtcacc cgagccgttc
    11581 tgaagtgttg cgtagcttcc tggacgatta atcggtaggc cggatcaggc gttacgccgc
    11641 acccggcact agtccctttg cacaaacgcc accttttcgg tggcgttttt tatcgcccac
    11701 gcactaccag cgcctggtcc agctcgcgat acgcttcaac cagtttctct agtgaaacgc
    11761 gacttaaacc gctgggattt ggcagcaccc aaatctgcgt cgaaccaatg gtgagcgttt
    11821 gtttccccca ctgtgcaccg cgctggctga atccctgttc atatgcttgt ttgcccagaa
    11881 tcgccaacgc ctgcggctga taatcttcaa ttttttcaat cagcttacgc ccacctgcgt
    11941 gcagctcctg ctttgaaact tcattggctt gcaccgttgg acggtctacc agtttggtga
    12001 cgccacaacg ataatccagc agatgctgtg cctcctgcgg cttcaactga cggtcggtaa
    12061 acccggcctg atatatcacc ttccagaagc gatttgctgg atgagcaaag ggaaaaccag
    12121 tcccagcgga tgaaagccca gggttgatac cgcaaaacac gacccgtaac cctggagcca
    12181 aaatatcctc aaccatattt actcctttct aacatcacca ggaaaagtat aacggattga
    12241 aaatacattg tttataaaaa cagcaggcgc acggtaatgg ctggattgcg acacggagtt
    12301 actttataat ccgctaccat ggccccttag ctcagtggtt agagcaggcg actcataatc
    12361 gcttggtcgc tggttcaagt ccagcagggg ccaccagata tagcaaaggc tgacgagaaa
    12421 tcgtcagcct ttttcttttt atatatcagt tactttgcgt gccagtaagc cgctgcacgt
    12481 acccgctgtg ggtcatactg ttccgcttca aagcggcggc ttaaattctt aacgacttta
    12541 ccttcgccgg ttatccagat gaagtaatca tcggcaggga tttgcatctg cgccagacgc
    12601 gcatctaccg cctgctcatc atgtgccagc cattcgatat taaaaccatc aaggtgcgcg
    12661 agataatcct gacaggcgtt atcccgcacg ctaaccagcg cactaacttg cggtttaacg
    12721 gcaagtttgc tcaacgtttc caggcggcgg cgcagtgcgg gcattccgga ttcatcgcag
    12781 acatacagct gatacgcgta atcttccggc accaccagcg aaccgcgcgg acctgccacc
    12841 gtaagtttat cgcccggttg cgcctgcatc gcccagccgc tggcgacccc accgtcgtga
    12901 ataaagaaat cgatcgccag ttcatggcgt agttcgtcat acagcggcgt atagtcacgc
    12961 gacggtgggc gtggtccttc cggccagacg atgccctctt ccgttaccgt tggcggcaca
    13021 aagtgagcgt caggttgagg aaagaagagt ttgctgtgat cgtcaaagcc gcgcgatgta
    13081 aaaccgtcca gcgcctcgcc gccgaggaca atgcgctgaa aaccggcgct gatgcgctca
    13141 acgcgtaaca cagtcagttc acggaagcgc agatcattgc gaacgcgctg cgggtagcgg
    13201 ggggagttat tcattgttat cgccttcgtg atagtaatca gatatatcta aataattttc
    13261 gtaaatgata atgattgtta atcatgataa atgcaagcga tttgtagaac tgatatatct
    13321 gaagtatgtg aaggcattca gtgccatagc aatgctgccg gatgcggcgt gaacgtctta
    13381 tccggcctgg atgtgaggtg cttccacgtt taaacatcat ttttgtactt gcagtaatgc
    13441 tcacatcaga tataaattag atatatctaa tcaagcaaaa ggaggctgat atgagccatc
    13501 atcacgaagg gtgttgtaaa catgaaggcc agccacgcca tgagggctgc tgcaaaggtg
    13561 agaagtcaga acacgagcac tgcggacacg gtcaccagca tgaacacggt caatgctgcg
    13621 gtggtcgcca cggtcgcggc ggcggtcgtc ggcaacgttt ctttggtcac ggtgaattgc
    13681 gtctggtgat tctggatatt ctctcgcgcg atgacagcca cggttacgaa ttgattaaag
    13741 cgattgagaa cctaacccag gggaattaca ccccaagccc gggcgtcatc tacccaacgc
    13801 tggattttct gcaggagcag tcgctgatta ccatccgcga agaggaaggt ggtaagaagc
    13861 agattgcgct gaccgaacaa ggcgcgcagt ggctggaaga aaaccgcgaa caggtggaga
    13921 tgattgaaga acgcatcaaa gcgcgttgcg ttggcgcggc gctgcgccag aacccgcaaa
    13981 tgaagcgggc gctggataat tttaaagcgg tgctggattt acacgtcaac cagagcgata
    14041 tcagtgatgc acaaataaaa aagatcattg cggtgatcga ccgcgccgct tttgatatta
    14101 cgcaactgga ttaatcgccg catccgccag tggcgcggtg caattgccgg atgcgacgct
    14161 tgacgcgcct tatccggcct acacccgcta cacaccccgc aggcctgata agatgcgcca
    14221 gcatcgcatc aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc
    14281 gtcaccgcgt cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc
    14341 tcttccacca gctcggcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc
    14401 aggctggaaa gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg
    14461 gtgacgtttt tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc
    14521 tgcgaaccgg attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca
    14581 gcattagcgc tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg
    14641 ccctgttcgc cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga
    14701 aacgcaatat cgttaatcag cgaagtaata gtgccaatgc gctgggtact gtcggcgata
    14761 tcgtccattg tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg
    14821 gcggtgatag aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc
    14881 gccatttggt tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt
    14941 tcgttcagtt catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc
    15001 tggcttgaga catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt
    15061 aatgtcagcc ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc
    15121 gccaccttca gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa
    15181 caagcgcttg ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca
    15241 acaaaccaaa gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc
    15301 cagcgaagcg ataatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta
    15361 tgaatacgct tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca
    15421 atctcttcat ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc
    15481 accatcggta ccgcattggc ccgcacccaa taatggtcac catttttgcg gcgatttttc
    15541 acgatgccgc tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct
    15601 tttggcatat ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta
    15661 aagccgctca cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta
    15721 gtggacatca gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga
    15781 gaagacatgt tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg
    15841 tcggccccgc tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta
    15901 aagcacaaat ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt
    15961 aattatcttg cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat
    16021 catcaaatcc atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat
    16081 taaatattgc acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg
    16141 gagtggcgca atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc
    16201 atattttgaa caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga
    16261 atctcattga gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga
    16321 ttgaatactt caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg
    16381 ccggcgggga ttacggagcc gtagagtggc aagcgggagg tttaaatacg cttgtcgaca
    16441 cccagggaca ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc
    16501 gtaatccagt tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc
    16561 aggagctgct cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg
    16621 gtaaactgaa atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga
    16681 agctggcgaa agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg
    16741 cgttccacgg taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac
    16801 cgtttatgcc actgctgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc
    16861 gcacggctct taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac
    16921 cgattcaggg tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta
    16981 agctatgcga tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc
    17041 gcacgggcaa gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg
    17101 ccaaagcgct cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt
    17161 tttcagttct gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg
    17221 cctgtgcggc ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg
    17281 ctgagcaaaa aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg
    17341 atctggtaca ggaagcgcgt ggtaaaggga tgttgatggc aattgagttt gttgataacg
    17401 aaatcggcta taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc
    17461 tcaataacgc caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg
    17521 aactggtgat caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag
    17581 cgtaatatca aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta
    17641 cgcccgcagg catcatccgc tccggcgtta acaacacgct ttcactgccg tcatcggttt
    17701 ccgcacacaa cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc
    17761 ataagaccac caccgttttc cccatcagct cttcttcgct gtagtacggc accaggctgg
    17821 tcacggtttg cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt
    17881 tttcatggcg tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag
    17941 cgtaagccac ggtttccatt gtttcctccc ttcgggtaaa attttactaa actatagcaa
    18001 aagtttttct caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtgag
    18061 ccgctgtgtt accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc
    18121 cgataagcac ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg
    18181 gcaacactaa aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc
    18241 ttaaatgacg atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc
    18301 gccgaaaagc tggagtacaa gaccagtagt gcccgtaaac tccagacagg tgcagtcaac
    18361 caacaccata ttctggctat ctacagctac caacaggagc tggagatcaa cgatccttac
    18421 tatctggcga tccgccacgg cattgaaact cagtgcgaaa agctgggcat tgagctcacc
    18481 aactgttatg aacacagcgg attaccagac attaaaaacg tcaccggtat tttaattgtc
    18541 ggcaaaccca cgcccgccct gcgcgccgcc gcctgtgcgt tgaccgacaa tatctgtttt
    18601 atcgactttc acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc
    18661 agtaaagaaa tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc
    18721 ggtgaagatg agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga
    18781 ctgaaacaag tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt
    18841 tatgaactgg caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct
    18901 tccgattcca ttgctatcgg cgtactgcgg gcaattcatg aacgaggcct gaacatccca
    18961 caggatattt cgcttatcag cgttaacgat atccccaccg cgcgatttac ctttccgccg
    19021 ctctccaccg tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat
    19081 gaaaaagccc gcgatggtcg cgcgctgccg ctgttagtct tcgttcccag caaattaaaa
    19141 ctgcgcggca cgacccgtta aatcccctta cacactgtcc ggcaatcgtt tttgccggac
    19201 agtgctgccg tttattttcg tgatccagtt aaagtaaatg catttacctg ctacttttta
    19261 gtaaaaattt tactaaactc cccagcaatt acacaaacta ccatcaccat gaatggttcc
    19321 gatttctctc taccgggagg ccctatgaat cgctgggaaa acattcagct cacccacgaa
    19381 aaccgacttg cgccgcgtgc gtactttttt tcatatgatt ctgttgcgca agcgcgtacc
    19441 tttgcccgcg aaaccagcag cctgtttctg cccttaagcg gtcagtggaa tttccacttt
    19501 tttgaccatc cgctgcaagt accagaagcc ttcacctctg agttaatggc tgactggggg
    19561 catattaccg tccccgccat gtggcaaatg gaaggtcacg gcaaactgca atataccgac
    19621 gaaggttttc cgttccccat cgatgtgccg tttgtcccca gcgataaccc aaccggtgcc
    19681 tatcaacgta ttttcaccct cagcgacggc tggcagggta aacagacgct gattaaattt
    19741 gacggcgtcg aaacctattt tgaagtctat gttaacggtc agtatgtggg tttcagcaag
    19801 ggcagtcgcc tgaccgcaga gtttgacatc agcgcgatgg ttaaaaccgg cgacaacctg
    19861 ttgtgtgtgc gcgtgatgca gtgggcggac tctacctacg tggaagacca ggatatgtgg
    19921 tggtcagcgg ggatcttccg cgatgtttat ctggtcggaa aacacctaac gcatattaac
    19981 gatttcactg tgcgtaccga ctttgacgaa gcctattgcg atgccacgct ttcctgcgaa
    20041 gtggtgctgg aaaatctcgc cgcctcccct gtcgtcacga cgctggaata taccctgttt
    20101 gatggcgaac gcgtggtgca cagcagcgcc attgatcatt tggcaattga aaaactgacc
    20161 agcgccagct ttgcttttac tgtcgaacag ccgcagcaat ggtcagcaga atccccttat
    20221 ctttaccatc tggtcatgac gctgaaagac gccaacggca acgttctgga agtggtgcca
    20281 caacgcgttg gcttccgtga tatcaaagtg cgcgacggtc tgttctggat caataaccgt
    20341 tatgtgatgc tgcacggcgt caaccgtcac gacaacgatc atcgcaaagg ccgcgccgtt
    20401 ggaatggatc gcgtcgagaa agatctccag ttgatgaagc agcacaatat caactccgtg
    20461 cgtaccgctc actacccgaa cgatccgcgt ttttacgaac tgtgtgatat ctacggcttg
    20521 tttgtgatgg cggaaaccga cgtcgaatcg cacggctttg ctaatgtcgg cgatatcagc
    20581 cgtattaccg acgatccgca gtgggaaaaa gtctacgtcg agcgcattgt tcgccatatt
    20641 cacgcgcaga aaaaccatcc gtcgatcatc atctggtcgc tgggcaatga atccggctat
    20701 ggctgtaaca tccgcgcgat gtaccacgca gcgaaggcgc tggatgacac gcgactggtg
    20761 cattacgaag aagatcgcga tgctgaagtg gtcgatatta tttccaccat gtacacccgc
    20821 gtgccgctga tgaatgagtt tggtgaatac ccgcatccga agccgcgcat catctgtgaa
    20881 tatgctcatg cgatggggaa cggaccaggc gggctgacgg agtaccagaa cgtcttctat
    20941 aagcacgatt gcattcaggg acattatgtc tgggaatggt gcgaccacgg gatccaggcg
    21001 caggatgaca acggcaacgt ctggtataaa ttcggcggcg actacggcga ctatcccaac
    21061 aactataact tctgtcttga tggtttgatc tattccgatc agacgccggg accgggcctg
    21121 aaagagtaca aacaggttat cgcgccggta aaaatccacg cgctggatct gactcgcggc
    21181 gagctgaaag tcgaaaataa actgtggttt accacgcttg atgactacac cctgcacgca
    21241 gaggtgcgcg tcgaaggtga aacgctcgcg acgcagcaga ttaaactgcg cgacgttgcg
    21301 ccgaacagcg aagccccctt gcagatcacg ctgccgcagc tggacgcccg cgaagcgttc
    21361 ctcaacatta cggtgaccaa agattcccgc acccgctaca gcgaagccgg gcattctatc
    21421 gccacttatc agttcccgct gaaggaaaac accgcgcaac cagtgccttt cgcaccaaat
    21481 aatgcgcgtc cgctgacgct ggaagacgat cgtttgagct gcaccgttcg cggctacaac
    21541 ttcgcgatca ccttctcaaa aatgagtggc aaaccgacat cctggcaggt gaatggcgag
    21601 tcgctgctga cccgcgagcc aaagatcaac ttcttcaagc caatgatcga caaccacaag
    21661 caggagtacg aagggctgtg gcaaccgaat catttgcaga tcatgcagga acatctgcgc
    21721 gactttgccg tagaacagag cgatggtgaa gtgttgatca tcagccgcac agttattgcc
    21781 ccgccggtgt ttgacttcgg gatgcgctgc acctacatct ggcgcatcgc tgccgatggc
    21841 caggttaacg tggcgctttc cggcgagcgt tacggcgact atccgcacat cattccgtgc
    21901 atcggtttca ccatggggat taacggcgaa tacgatcagg tggcgtatta cggtcgtgga
    21961 ccgggcgaaa actacgccga cagccagcag gctaacatca tcgatatctg gcgcagcacc
    22021 gtcgatgcca tgttcgagaa ctatcccttc ccgcagaaca acggcaaccg tcagcatgtc
    22081 cgctggacgg cactgactaa ccgccacggc aacggtctgc tggtggttcc gcagcgccca
    22141 attaacttca gcgcctggcg ctatacccag gaaaacatcc acgctgccca gcactgtaac
    22201 gagctgcagc gcagtgatga catcaccctg aacctcgacc accagctgct tggcctcggc
    22261 tccaactcct ggggcagcga ggtgctggac tcctggcgcg tctggttccg tgacttcagc
    22321 tacggcttta cgttgctgcc ggtttctggc ggagaagcta ccgcgcaaag cctggcgtcg
    22381 tatgagttcg gcgcagggtt cttttccacg aatttgcaca gcgagaataa gcaatgagga
    22441 tcatcgataa cttagaacag ttccgccaga tttacgcctc tggcaagaag tggcaacgct
    22501 gcgttgaagc gattgaaaat atcgacaaca ttcagcctgg cgtcgcccac tccatcggtg
    22561 actcattgac ttaccgcgtg gagacagact ccgcgaccga tgcgctattt accgggcatc
    22621 gacgctattt tgaagtgcat tactacctgc aagggcagca aaaaattgaa tatgcgccga
    22681 aagagacatt acaggtagtg gaatattatc gtgatgaaac tgaccgtgaa tatttaaaag
    22741 gctgcggaga aaccgttgag gtccacgaag ggcaaatcgt tatttgcgat atccatgaag
    22801 cgtatcggtt tatctgcaat aacgcggtca aaaaagtggt tctcaaagtc accatcgaag
    22861 atggttattt ccataacaaa taacaactac ggcggcaaaa ggagtttgcc gccaccgcta
    22921 ccctacacat tttcggagat gtgttatgtc tgataccaaa cgtaatacaa tcggcaaatt
    22981 tggcttgctc tcgctgactt ttgccgccgt ttacagcttt aacaacgtta tcaataataa
    23041 tattgagctt ggactggcct cggcaccgat gtttttcctc gcgacgattt tttattttat
    23101 tcccttctgt ctgatcatcg cagaatttgt ttcgttaaat aaaaactcag aagccggtgt
    23161 ctacgcgtgg gtaaaaagtt cgctgggcgg acgttgggca tttattactg cctataccta
    23221 ctggttcgta aacctgttct ttttcacctc actgttgccg cgcgttattg cttatgcttc
    23281 gtatgccttc ctcggctacg aatatattat gacgccggtt gccaccacca ttatcagtat
    23341 ggtgctgttc gccttctcca cctgggtttc caccaacggg gcgaaaatgt tggggccaat
    23401 aacctccgtc acttcaacgc tgatgctgct gttaacgctc tcctacattt tactggcagg
    23461 tacggcgctg gttggcggcg tacagcctgc tgaccccatc accgttgacg cgatgatccc
    23521 gaacttcaac tgggcgttcc tcggcgttac cacctggatc tttatggccg caggtggcgc
    23581 ggagtccgtc gctgtgtacg ttaacgacgt caaaggcggt tcgaaatcgt tcgttaaagt
    23641 gatcatcctc gccgggattt ttatcggcgt actgtattcc gtctcctcgg tgctgattaa
    23701 cgtcttcgtc agcagcaaag agttgaaatt taccggcgga tcggtgcagg tattccacgg
    23761 catggcggcg tattttggtc taccggaagc gttgatgaat cgctttgtcg gtctggtgtc
    23821 ctttaccgcg atgttcggtt ccctgctgat gtggaccgca acgccggtga aaattttctt
    23881 ctccgaaatc ccggaaggca tctttggtaa gaaaaccgtc gaactgaacg aaaacggcgt
    23941 tccagcgcgc gcagcgtgga tccagttcct gatcgtcatc ccgctgatga ttatcccgat
    24001 gctcggttcc aataccgtgc aggatctgat gaatactatt attaatatga ctgccgcagc
    24061 gtccatgctt ccgccgttat tcatcatgct ggcttacctg aatttacgcg ccaaattaga
    24121 tcacctgcca cgcgatttcc gcatgggctc ccgccgcacc ggtattatcg ttgtttcaat
    24181 gctgattgcg atatttgccg tagggtttgt cgcttcgaca ttcccgactg gcgcgaatat
    24241 tctgaccatc attttttata acgtcggcgg tattgttatc ttcctcggct ttgcgtggtg
    24301 gaaatacagt aaatatataa agggattaac ggctgaagag cgccatattg aagcgacgcc
    24361 agccagcaat gttgattgag cagactaata aaaaatgagc aggcattttc cctcttcctg
    24421 gatggggagg gggaaaatag tttctgcctt tatattttta taattacaac gataaaaggc
    24481 tgtacttttt ctttagctca tggattaaca caatgaaatt aatcactgca ccatgcagag
    24541 cattacttgc tctgccgttt tgctacgcct tttctgcggc aggagaagaa gcacgtccgg
    24601 cagaacatga cgacacaaaa acacctgcaa ttacctcgac atcttctcct tcatttcgtt
    24661 tttacggcga attaggggtt ggtggatata tggatttaga gggtgagaat aaacataaat
    24721 acagcgacgg tacctatatt gaaggtggcc tggagatgaa gtacggctcc tggttcggcc
    24781 tgatttacgg cgaaggctgg accgtgcagg ccgaccacga cggcaatgcc tgggtgccag
    24841 accatagctg gggtggtttc gagggcggaa ttaaccgttt ctatggcggt tatcgtacca
    24901 atgatggcac cgaaatcatg ctcagtctgc gtcaggattc ctcgctggat gacctgcaat
    24961 ggtggggcga cttcaccccc gatctgggct acgtcattcc caatacccgc gacattatga
    25021 ccgcgctgaa ggtgcagaac ttaagcggca actttcgtta tagcgtcacc gccacacctg
    25081 ccggacatca tgacgaaagc aaagcctggc tacattttgg caagtacgat cgctatgacg
    25141 acaaatacac ctatccggca atgatgaacg gttacatcca gtatgacctt gccgaaggca
    25201 tcacctggat gaacggtctg gaaatcaccg acggcacagg acagctctat ctcacgggcc
    25261 tgctaactcc taactttgcc gctcgcgcct ggcaccatac cggacgcgcc gacgggctgg
    25321 acgtaccggg aagtgaaagt gggatgatgg tgagcgccat gtatgaagcg ttaaagggcg
    25381 tttatctctc caccgcttac acctacgcca aacatcgccc tgaccacgct gacgatgaaa
    25441 ccacctcttt catgcagttt ggtatctggt acgaatacgg cggcggacgt ttcgccacgg
    25501 cttttgatag ccgcttctac atgaaaaatg cctctcacga tcccagcgac caaatcttcc
    25561 tgatgcaata tttctactgg taataaggac tgtaatttca tgaaaataaa aactatttta
    25621 acgccagtaa cctgcgctct gctgataagt ttttccgccc atgccgctaa cgccgacaat
    25681 tataaaaacg tgattaaccg tactggcgcg ccgcagtaca tgaaggatta cgattacgac
    25741 gatcaccagc gttttaatcc gtttttcgat ctcggagcct ggcatggtca tctgttgcca
    25801 gacggcccta acaccatggg cggctttccg ggcgttgcgc tgctgacgga agagtacatc
    25861 aactttatgg ccagcaattt cgaccgcctg accgtctggc aggacggcaa gaaagtcgac
    25921 tttacgctgg aggcatacag tattcccggt gcgctggtgc aaaaactgac agcaaaagat
    25981 gtgcaagtcg aaatgactct gcgcttcgcc acgccgcgca cgtcactact ggaaaccaaa
    26041 atcaccagca ataaaccgct ggatctggtg tgggacggcg aactgctgga aaaactggaa
    26101 gcgaaagaag ggaaaccgct ttccgataaa accattgctg gcgaataccc tgactatcag
    26161 cgcaaaatca gcgccacccg tgatggcctg aaagtcacct ttggcaaagt gcgcgccacc
    26221 tgggatctgc tgacctccgg cgaatcggaa tatcaggtgc ataaatccct gccggtgcag
    26281 actgaaatca acggtaatcg ctttaccagt aaggcgcata tcaacggttc gaccacgctc
    26341 tacaccacct attcccatct gctgaccgct caggaagtta gcaaagagca aatgcagatc
    26401 cgcgatattc tggcgcgtcc ggcgttttat ctcaccgcct cgcagcaacg ctgggaagaa
    26461 tacctgaaga aagggttaac caatccggat gcgacgccgg aacagacgcg cgtcgcggtg
    26521 aaagcgatcg aaacgctcaa cggtaactgg cgctcaccgg gcggtgcggt gaaatttaac
    26581 accgttacac cgtcggtgac cgggcgctgg ttctccggca atcagacctg gccgtgggat
    26641 acctggaagc aggcgtttgc gatggcgcat ttcaatccgg acatcgccaa agagaatatc
    26701 cgcgcggtct tctcctggca gatccagcct ggcgatagcg tgcgtccgca ggatgtgggc
    26761 tttgtccccg acctgatagc gtggaacctt agccccgagc gtggtggcga tggcggcaac
    26821 tggaacgaac gtaataccaa acccagcctt gccgcctggt cggtgatgga agtgtacaac
    26881 gtcacccagg ataaagcctg gttggcagag atgtacccga aactggtggc ctatcacgac
    26941 tggtggttac gtaaccgcga tcacaacggc aacggcgtgc cggaatatgg cgcgacccgc
    27001 gacaaagccc acaacactga gagcggcgag atgctgttta cggtgaagaa aggcaacaaa
    27061 gaagagacgc agtctgggct gaacaactac gcccgcgtgg tggagaaagg ccagtacgac
    27121 agtctggaaa ttccggcaca ggtcgctgcg tcgtgggaat cggggcgtga tgatgccgcc
    27181 gtctttggtt ttatcgacaa agaacagttg gataaatatg tcgctaatgg cggcaaacgt
    27241 agcgactgga cggtgaaatt cgccgaaaac cgcagtcagg acggaacgtt gctgggctac
    27301 tcgctattgc aggagtcggt ggatcaggcc agctatatgt acagcgataa ccattatctg
    27361 gcggagatgg caaccatcct cggtaagcca gaagaggcca aacgctatcg ccagttggca
    27421 cagcagctcg cggactacat caacacctgt atgttcgacc cgactaccca gttctactat
    27481 gacgtacgta ttgaagataa accactggcg aacggctgcg cgggcaaacc gattgttgag
    27541 cgcggtaaag ggccggaagg ctggtcgccg ctgtttaacg gtgcggcaac gcaggccaat
    27601 gccgacgcgg tggtgaaggt gatgctcgat cctaaagagt tcaacacctt tgtcccgctg
    27661 ggaacggcgg cgttaaccaa tccggctttt ggcgctgata tctactggcg tgggcgcgta
    27721 tgggtggatc agttctggtt tggtctgaaa gggatggagc gttacggtta tcgcgatgat
    27781 gccctgaaac tggcggatac gttcttccgt cacgccaaag gattaaccgc cgatggccca
    27841 attcaggaga attacaaccc gctgactggc gcacagcaag gcgcaccaaa tttctcctgg
    27901 agtgccgcgc atttgtatat gttgtataac gattttttcc gtaagcaata atttgcaact
    27961 gctggcggat gcggcgtaaa cgccttatcc gccctacatg tgcaatcctc ctggccggat
    28021 aaaacgcgac aagcgtcgca tccggcatct gcaccgtgcc actggcggat gcggcgtgaa
    28081 cgccttatcc gccctacatg tgcaatcccc caggccggat aaaacgcggc aagcgtcgca
    28141 tccggcgtta ccaccgggcg tattcttttt gaatcccatc acaaaccccg cactcccctt
    28201 ttcccttttc tccggcgacg gctaaattag aactcatccg accacataac aattatttta
    28261 catactggac aattttatga gctacccgtc gctgttcgcc ccgctggatt taggttttac
    28321 cacgttaaaa aaccgcgtgt tgatgggctc aatgcacacc gggctggagg aatacccggc
    28381 cggtgccgag cggctggcag cgttttatgc cgaacgcgcc cgtcacggcg tggcgctgat
    28441 tgtcagcggc ggcattgcac cagatttaac aggcgttggc atggaaggtg gcgcaatgct
    28501 caacgacgcc agccagatcc cacaccatcg caccattacc gaagcggtac atcaggaagg
    28561 cggcaaaata gccctgcaaa ttttgcatac cgggcgctac agctaccaac cgcatctggt
    28621 cgctccctcc gcattgcagg cccccatcaa ccgtttcgtt cctcacgaac tcacgcatga
    28681 agagatcctg caactgatcg acgatttcgc ccactgcgcg caactggcac gggaggcagg
    28741 atacgacggt gtagaggtaa tggggtccga agggtatttg atcaacgaat ttctgacgct
    28801 gcgcaccaat cagcgtagtg accagtgggg cggcgattac cgcaaccgga tgcgatttgc
    28861 cgtagaagta gtgcgtacgg tgcgcgaacg cgtcggcaac gacttcatta ttatctaccg
    28921 gttgtcgatg ctcgacctgg tcgaaggcgg cggcactttt gccgaaacgg tagaactggc
    28981 gcaggccatt gaagcggcgg gcgcgaccat tatcaacacc ggcattggct ggcatgaagc
    29041 acgtattccg accattgcca cgcccgtgcc gcgcggcgca tttacttggg tcacgcgcaa
    29101 actgaaaggc cacgtctcgc tgccgctggt gaccaccaac cggattaacg atccgcaggt
    29161 tgccgacgac attctctcgc gcggcgatgc cgatatggtg tcgatggcac gaccgtttct
    29221 tgctgatgcg gaactgctgt caaaagcgca atcggggcga gccgatgaga tcaacacctg
    29281 cattggctgc aatcaggcct gcctcgatca gatcttcgtt ggcaaagtca cctcgtgcct
    29341 ggtgaatcct cgcgcctgcc acgaaaccaa aatgccaatc cttcctgccg tgcagaaaaa
    29401 aaatctggcg gtggtcggtg cgggacctgc tgggctggcg tttgccatta acgcggcggc
    29461 acgtgggcat caggtaacat tgtttgatgc ccacagcgag atcggcgggc agtttaatat
    29521 cgccaaacag atccccggca aagaggagtt ttacgaaacg ctgcgctatt accaccggat
    29581 gatcgaagtg acgggcgtga cgctgaaact caatcacacc gtaacggcgg atcagttaca
    29641 ggcgttcgat gaaacgatcc tcgccagtgg gatcgtgccg cgcgttccgc ctatcgacgg
    29701 gatcgatcat ccgaaggtat tgagttatct cgatgtactg cgcgacaaag cgccggtagg
    29761 caacaaggtt gccatcatcg gttgtggcgg gattggtttt gatacggcga tgtatttaag
    29821 tcagccgggc gaatccacca gccagaatat cgccaggttc tgtaatgagt gggggatcga
    29881 cagcagccta caacaggctg gtggcttaag cccgcaggga atgcagatcc cgcgtagccc
    29941 acggcaaatt gtgatgctcc agcgcaaagc cagcaaaccg ggacaggggt taggcaaaac
    30001 caccggctgg atccatcgca ccaccctgct ctcgcggggt gtgaaaatga tcccaggcgt
    30061 aagttatcag aagattgacg atgacgggct gcatgtggtg atcaacggcg aaacgcaggt
    30121 attagcagtg gacaatgtgg tgatctgcgc agggcaagag ccaaaccgcg cgctggcgca
    30181 accgctgatt gatagcggga aaactgtgca tttaattggc ggctgcgatg tggctatgga
    30241 gctggacgca cgacgggcaa ttgcccaggg aacacggctg gcgctggaga tttaaatcgt
    30301 tatttgaagc gccggatg
//
LOCUS       DAZGXZ010000054        30204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000054 DAZGXZ010000000
VERSION     DAZGXZ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.054"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKJF_002505"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKJF_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP0949845.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKJF_002506"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKJF_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0949846.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKJF_002507"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKJF_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP0949847.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHKJF_002508"
     CDS             complement(1087..1581)
                     /locus_tag="ACHKJF_002508"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP0949848.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKJF_002509"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKJF_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP0949849.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKJF_002510"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKJF_002510"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP0949850.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKJF_002511"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKJF_002511"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP0949851.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKJF_002512"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKJF_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP0949852.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKJF_002513"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKJF_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP0949853.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKJF_002514"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKJF_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP0949854.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKJF_002515"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKJF_002515"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP0949855.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHKJF_002516"
     CDS             complement(9275..9769)
                     /locus_tag="ACHKJF_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP0949856.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKJF_002517"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKJF_002517"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0949857.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKJF_002518"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKJF_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP0949858.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKJF_002519"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKJF_002519"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0949859.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHKJF_002520"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHKJF_002520"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP0949860.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKJF_002521"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKJF_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP0949861.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKJF_002522"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKJF_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP0949862.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKJF_002523"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKJF_002523"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP0949863.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLIREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKRLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKJF_002524"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKJF_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP0949864.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17948..18874
                     /locus_tag="ACHKJF_002525"
     CDS             17948..18874
                     /locus_tag="ACHKJF_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP0949865.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKJF_002526"
     CDS             18925..19182
                     /gene="yqhH"
                     /locus_tag="ACHKJF_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP0949866.1"
                     /translation="MKTIFTVGVVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19225..21444)
                     /locus_tag="ACHKJF_002527"
     CDS             complement(19225..21444)
                     /locus_tag="ACHKJF_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP0949867.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKJF_002528"
     CDS             complement(21555..22967)
                     /gene="ftsP"
                     /locus_tag="ACHKJF_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP0949868.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKJF_002529"
                     /gene_synonym="parF"
     CDS             complement(23042..23779)
                     /gene="plsC"
                     /locus_tag="ACHKJF_002529"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP0949869.1"
                     /translation="MLYIFRLIITLIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKJF_002530"
     CDS             complement(24012..26270)
                     /gene="parC"
                     /locus_tag="ACHKJF_002530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP0949870.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26816..27298)
                     /locus_tag="ACHKJF_002531"
     CDS             complement(26816..27298)
                     /locus_tag="ACHKJF_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP0949871.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKJF_002532"
     CDS             complement(27351..27743)
                     /gene="ygiW"
                     /locus_tag="ACHKJF_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP0949872.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKJF_002533"
     CDS             27895..28554
                     /gene="qseB"
                     /locus_tag="ACHKJF_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP0949873.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKJF_002534"
     CDS             28551..29900
                     /gene="qseC"
                     /locus_tag="ACHKJF_002534"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP0949874.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATHLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29946..>30204)
                     /locus_tag="ACHKJF_002535"
     CDS             complement(29946..>30204)
                     /locus_tag="ACHKJF_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFP0949875.1"
                     /translation="FFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTPLI
                     ILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7449 a         8075 c         7638 g         7042 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagtag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccgtgaca gagcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagag
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catgctgtgc gaaagcggcg acatcagcga acaggagaga
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tagtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgccagatt cacgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcacc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcgcg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcgacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgac
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcagca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc ggcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac cgcctggtag
     1801 ttactgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggcttcac aaaggtggta
     1921 tgatcgcctt taccgatatt ggtgatcagt gcactgtatt gattttgcag gatatcctgc
     1981 aattcgcagc agtgaacggt acgaccaata atacggccca gcgtagagtg cagttgcgcc
     2041 acttcaagcg tgttaccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagact cgcgtcctgc cgccagtttc accagcatgt tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggtgcct gcggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttac tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggca ataactgcgg tatcaacctt ataaacctgt
     2521 tcaacaaagt cgctcagctt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 agcacgccca aaccgtcgag gttgatcggg ttcgcgacac cacctaccgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgcc
     2761 gggtgacccc agtagccatt ggcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc ggactgttca ggtgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat gtccacccag
     2941 tccagcgccg aaagctgata gaaatgaaca atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacg accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttaacaa
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagc gaccccattg
     3901 aagactggtg catggtcggc agcagcgcac cgagcgcgat gatgaagaac atcaccttgt
     3961 ttagtcgctg tagcgacacc ttccacccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg gcccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccacccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtgccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accgaaaatc accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcagac cgcctttatc gagacgttcc acacctttct ggttgcacag ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtacgg acaggcgacc
     4981 atgcagtaac ggcagccggt gcacacgtct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gtgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgccg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacga
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc ttattcgata tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctgc cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagctggtt
     9061 gttgaaagat gatcgcgtga cgtcggtatt gattggtgcc agccgcgcgg agcaactaga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcatct aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggtcgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcacaa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattcctga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc ctatctggac ggcactgcaa atttctggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaaa cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcggcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac atgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 atccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gacatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgtgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggca aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc ggattgtata acattgccac ttttggacaa
    13741 ttttgcagac attttattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgattca ggagtacaat aggcaaataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccagg gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgtttcc agtttttcaa ctggttaagc
    14341 ggattgctgt gggtcagcgt ggtaaccagt ttcggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc tgttcccggt tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctggccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cggctggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttaattttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggaggaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tgattcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gcggctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgacg atgagcgtat
    16621 tgacgccgcg attgccgcaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gttgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tacaagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactcca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc atccgctgat gcaacaacgc cagctacacg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaaccccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg tattgccgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgcccgttg ttactcttcc ttgttcagga atgccaaata taaggacatc atcatgcaga
    17881 gccggaagct cttaaaagaa caactcatct atatccggga taaacgcaac ggagaggtga
    17941 aaaacagatg aaaataatac ttctgttttt agcagccctg gcaagtttta ccgtacacgc
    18001 acagcccccc tcactgaccg tagaacaaac agtccggcat atttatcaga actataaatc
    18061 agatgccact gccccttatt ttggtgaaac cggagagcgg gcgataactt ctgcgcgtat
    18121 tcaacaggcg cttaccctga acgacaatct tacgctgccg ggcaatattg gctggctgga
    18181 ttatgatccg gtttgtgatt gtcaggattt tggcgatctg gtgctagaaa gcgtagcgat
    18241 aactcaaact gacgccgatc atgccgatgc cgttgtgcgc tttcgtatct ttaaagatga
    18301 taaagaaaag accacgcaga cactgaaaat ggtggcggaa aatggtcgtt gggtcattga
    18361 cgatattgtc agcaatcatg gcagcgtctt acaagcagtt aatagcgaga atgaaaaaac
    18421 gctggccgct ttagcttcgt tgcaaaaaga acagccggaa gcctttgttg ccgaactctt
    18481 tgaacatatt gctgattata gctggccgtg gacgtgggtg gtttccgact cttaccgcca
    18541 ggcggttaat gccttctata aaaccacctt caagacggcc aataatcccg atgaagatat
    18601 gcaaatagaa cggcaattta tttacgacaa tccgatctgt tttggcgaag agtcgctatt
    18661 ttcacgcgtt gatgaaattc gagtcctgga gaaaaccgcc gattccgccc gcattcatgt
    18721 tcgttttacg ctgaccaatg gcaacaacga agagcaagaa ctggttttac agcggcgcga
    18781 aggcaagtgg gaaatcgctg attttatccg cccgaacagc ggcagcctac ttaagcagat
    18841 tgaggcaaaa accgccgcca gattaaagca atgagctgaa ttaaataaca attagccgga
    18901 acaataaata aaagggaaca ctatatgaaa acgattttca ccgtgggagt tgttgttctg
    18961 gcaacctgct tgctcagtgg ctgcgtcaat gagcaaaagg tcaatcagct ggcgagcaat
    19021 gtgcaaacat taaatgccaa aatcgcccgg cttgagcagg atatgaaagc actacgccca
    19081 caaatctatg ctgccaaatc cgaagctaac agagccaata cgcgtcttga tgctcaggac
    19141 tattttgatt gcctgcgctg cttgcgtatg tacgcagaat gataaaaaaa tccccggcag
    19201 catgtcagtt gccggggatt ttttttaacg tccaaccgcc gctttagggc gtttcttcgc
    19261 accagcattc accggacgag attgcgtaga cgacgctttt tttgccgtag caggcgtctg
    19321 acgctgggtc gccatcggcg tatgtttcgt caacgccgga cgggtattgc ggttctggcg
    19381 acgagcttca cgcatctctt caatggttgg cgcaggcact aagcaatcgc gacggctgcc
    19441 aatcagatgc tttttgccca tcgcttccag cgcctggcgg attaacggcc agtttgccgg
    19501 atcgtggtaa cgcaacaacg ctttatgcaa acgacgctgt ttgtcgccct tcggtacgaa
    19561 gacgtcttca ctcttataac caatcttcgc cagcgggttt ttcccggtgt aatacatggt
    19621 ggttgagttc gccagcggcg atgggtagaa gttctgtacc tggtcgagac ggaaacgatg
    19681 ctttttcagc cacagcgcca gattcaccat atcttcatca cgcgtaccgg ggtgcgcgga
    19741 gatgaaatag gggatcagat actgctcttt acctgcctgt tttgagtaag tatcgaacag
    19801 ctctttaaag cggtcatagc tgcccatgcc cggcttcatc atcttcgata acggcccttc
    19861 ttcggtatgt tccggggcaa tcttcagata accgccgacg tgatgggtcg ccaactcttt
    19921 gatatagcgc ggatcttcca cggcgatgtc ataacgtacg cctgaggcga tgaggatttt
    19981 tttaatgcct ttcagatcac gcgcacggcg atagaggttg atcgtcggtt cgtggttagt
    20041 gtccatgtgc ggacaaatat ccggataaac gcacgacaaa cggcgacaag tttgttcagc
    20101 gcgtggcgat ttgcagcgca acatatacat gttggcagtt ggcccaccga gatcggaaat
    20161 tacgcccgta aaaccaggaa cggtgtcgcg gatcgcttcg atctcattaa tgatcgaatc
    20221 ttccgaacgg ctctgaataa tgcgcccttc gtgctcggtg atagaacaga aagagcagcc
    20281 gccaaagcag ccgcgcataa tgttgaccga gaaacggatc atttcgtaag ccggaatacg
    20341 ggcattgcca taggccggat gcggcacgcg cttgtacggc agcgcaaaaa cgctgtccat
    20401 ctcttcggta gaaagcggga tagcaggcgg gttgatccac acatagcggt caccgtgttt
    20461 ttgcatcaat gcgcgggcgc agcctgggtt ggtttcgtgg tgcagaatgc gcgaagcatg
    20521 agcgtacagg actttatcgc ccttcacttt ctcgaaagaa ggcagcaata cgtaggtttt
    20581 ttcccacggt ttcgggcgcg gcggctgcac ggttacagct ttcgcttcct gctttttcgg
    20641 cgcaacaggt ttgttatccg cgcacggcag atcttcgcca tacggatgcg ggattgggtc
    20701 gattttccct ggggtatcaa gacgagtaga atccacaccg ctccagccag gcagcgcctc
    20761 ttttacgata attgcggtat tacgcacatc gcggatttcg ctaattggct cgcccatcgc
    20821 cagacgatgc gctacttcca ccagcggacg ttcaccgtta ccaaacatca gcatgtcggc
    20881 tttcgaatcc accagcacgg aacggcgcac ggtatcggac cagtaatcat aatgcgcggt
    20941 acggcgcaga ctagcctcaa taccgccaag gatcaccggc acatctttcc acgcctcttt
    21001 acaacgctgg gtataaacca gtgtggcgcg atccggtcgc ttacccgcga cgttatccgg
    21061 tgtgtaggca tcgtcatgac gtaaacggcg atcggcggta tagcggttga tcatcgaatc
    21121 catgttgcca gcagtaacac caaaaaacag attcggttta cccagacgca taaagtcgtc
    21181 tttgctgctc cagtccggct gggcgatgat cccgacgcga aagccctgcg cttccagcat
    21241 acgaccgcaa atcgccatcc cgaagcttgg gtgatcgaca tacgcgtcgc cagtaaccaa
    21301 aatgatgtcg cagctatccc agccaagttg atccatctct tcacgagaca tcggcaaaaa
    21361 cggtgccggt ccaaaacagg cggcccagta ctgcggccag gagaacaggt cgcgatccgg
    21421 ttggatcagg gagatagagc tcattttgct tccagaaatg ataaaaaaat aatcaaaggc
    21481 cggggattat aagccggaac gaaagagaaa tcgaaaggta ttccatactc gccctcctcg
    21541 ggcgagtatg aagattacgg taccggattg accaacagtt gcccaatcga gccacggtcc
    21601 gccatttcca gcgtctgact gttgaagtag aacgggaagt gcgcccagga aggctgaccg
    21661 aaataaacaa gcagctccac ttgtccgtca acccaaacgg tatctttcca gcctctgtct
    21721 tccgggaacg gcatcgcgcc attcacgtta cggatctgga acatcacgcc ttcaatatgg
    21781 aacgcttgcg gctcgtccgc gcgtaccgtc cagcgttccc acgttccttg ctgcgcggtg
    21841 acatcaatac ggttgacgtc ccacagctgt ccattaatac ccgggtcatc acccagacta
    21901 atatcgcgac tgcgaatcgg cgaaccggcc atgatttcag ttggcagcaa gcgcatcgga
    21961 agactgtctg tgaccagcgg cagaaggccg gttgggcgca gcgttagcac cagggtagaa
    22021 accaaaatgc tggatggctc aaagaagcca cgaatacgat caacaatgct cgccgcttcg
    22081 ccacaggtga tcgacacttc atcaccgttg ctcatatcca ccagaatctc gcggcgctcg
    22141 cccggtgcca gcgaaagttg cttcaccgat acaggagcag gcaggaatcc ctgatcgcca
    22201 gaaatcacat gtaacgggcg accatcgctc atctgtagtt gatagcgacg agagttcgac
    22261 gcgttcagca gtcgcaagcg cacccaacca cgcgagactt caacgtacgg gctttgtaca
    22321 ccgttaacca gcagcgtatc accaacaaag ccgccgcttc ccggttcgtt gtattctggc
    22381 gtaccaaagt tatccagccg tttatcctgg ataatgaccg gaaaatcatc cacaccataa
    22441 tggttgggga taggcagcga cttgctgact tcatcttcca ccagccacat tccggcaagg
    22501 ccgttataga cctgctgagc cgtgcggttg ggagtattgg cgtgatacca cagagtagct
    22561 gcgttctggc gaatgggcag tacgggtgcc cagtcagcgt ttggcgacat catccgtgcc
    22621 ggaccgccca tcagcgggcc tggtacctgt agcccggcca ccgtcattga gacattttct
    22681 gtcaggcggt tgctgtaaat aagcttaaca tcgtcgccct tccagacgcg gatagtcggc
    22741 cccaggtaac gaccattgat tccccagacc gacgcgcgtg tccctggcgt aaatgaccag
    22801 tgcgcacgtt gtacagtcat aaacagcggt tgcccacggc gagattcaag tagcggcgga
    22861 acgggtagcg gttgctgttg cccggctgcg ctggccttca ggggaacagc gcctgcacaa
    22921 agtgcaatcc ccgatgcctg aatgaactga cgccgactga gtgacatatt tgctccatgt
    22981 aaaactggct aaagtaatac cgtgcaggaa agtgttcccc gcaaaaatac agattgccga
    23041 cttaaacttt tccggcggct tcgcgttctg cgacttcttt atcgagctcg gcgatttttt
    23101 gttccattat cgaacgacaa tgggcagcca gctcacgaac ctgatctttg ccatactgac
    23161 tgacgtcaat tggcggcagc atttcgacaa tcaccagacc gttgtgcagt cgattaagat
    23221 taatcttatt cgaagttgta gagacgcaca cgggaataat cgggacgccc gccgcaattg
    23281 ccgcgtgaaa tgctccagtc ttgaacggta gcaggccgcg accacggctg cgggttcctt
    23341 ccgggaacat ccagatggaa atacggcgtt ttttgaaatg attcacgact tccgcaatgg
    23401 tgccgtgagc tttagtgcga ttgtttctgt cgatcaataa gttgccggtt aaccagtaca
    23461 actgcccgaa gaaggggatc cacagcaagc tctttttacc taccgtcacc gtcggcggtt
    23521 gcacaatgtt cgatgctgtc accatgtcat agttgttctg gtggttagcg atatagatag
    23581 cattgccgta gctttcagca tctgcaggtt tgcggcactc aactttcagg ccgaacagcg
    23641 gcgcaagacg gccaaacata tgcccaaagg tggccacatg tttcgggtta cgcgggctga
    23701 aaaggcagta aatggagccg aatacacaga ctaagatgct gtaaatcaag gtaataataa
    23761 gacgaaagat atatagcata acaccctctg aaggtctgag agactggcat tgtacgtcac
    23821 ctgaaatcag gttagggcat tgttaaaagc cgctctctgg aaaagcgtat tgttaatcgc
    23881 aacgcatgaa taaacaacgg tttcaaggga atttttatcg tttccggggg ttgaatacgc
    23941 tgccggatga cgacttaacg tttcatccgg cgttccttgc aagcgggagg aaacaggccc
    24001 tccccggcat attactcttc gctatcaccg ctgctggcac ggcgaggaga gtcgatctca
    24061 acacgatcga tacgctgcaa accgcgcatc aacgtaccgc ggcgtccacg ttcgccagtg
    24121 actttctgta actcttccgg gcgcagttta attttgcgtt tcccaacatg aatggtcagc
    24181 gtgctttgcg gcggcagaac gtacaattgc gccagaccat cttctccacg cgcggcttct
    24241 gccgatggaa tgttgataat cttgttgcct ttgcccttcg acagctgcgg cagatcgctt
    24301 accgggaaca tcaacatacg gcctgcctga gtgattgcca gcagcatatc ggaagcatct
    24361 tcaatcacca ccggcggcat aacatgggca ttttccggta aggtgatcaa agccttacct
    24421 gcacggttac gcgccaccag atcgttaaag gtgcagacga aaccgtaacc cgcatcggaa
    24481 gccatcagca gtttctgatc gtcgctttcc atcagcatat ggtcaacggt cgccccaggc
    24541 ggcaacgtta atttgccggt gagtggttcg ccctgaccac gcgccgacgg cagcgtaatc
    24601 gggtcgatgg catagctacg accggtggaa tcaacaaaca ctaccggttg gttgctctta
    24661 cctttcaccg ccgctttgaa gctatcaccc gctttataat tcaggcccgg cgcgtcgata
    24721 tcatggcctt tagcgctgcg tacccagccc atctgcgaca gcacaatggt gacaggttca
    24781 gacggcagca tgtcgtgctc gctcatcgct ttcgcttctt cgcgttcctg caacggcgaa
    24841 cgacgatcgt caccgtaggc ttgcgcgtct gcctgcagtt ctttcttcag caggttattc
    24901 attttacgct cggaagccaa aatgccctgc aactggtcgc gctctttttc cagctcactc
    24961 tgctcaccgc gaatcttcat ctcttccagt ttggcaagat gacgcagttt cagttcgagg
    25021 atcgcttccg cctgggtttc cgtaaggcca aaccgcgaca tcagcgccgg tttcggttca
    25081 tcttcgttac gaatgatctc aatcacttcg tcgatgttga gaaacgccac cagcaaacct
    25141 tccaggatat gcaggcgctt gaggactttc tccagacgat agttcagtcg gcggcgcacg
    25201 gtatcgcgac ggaacaccag ccattcggag aggatttcca gcaggttttt caccgccgga
    25261 cgaccatcca gaccgatcat attgaggtta atgcgatagc tcttttccag atcggtggta
    25321 gcgaagaggt ggttcatcac ctgatccata tccacgcggt tggaacgcgg cacaatcacc
    25381 agacgggtcg ggttctcgtg gtcagattca tcgcgcagat cgtcaaccat cggcagcttt
    25441 ttgttgcgca tttgcgcagc aatttgctcc agtacgcgcg cacctgaaac ctgatgcggt
    25501 aatgcgctga taaccaccgc gccatcttct ttcttccaca ccgcgcgcat acgcactgaa
    25561 ccacgtccgt tctcgtagat tttacggatc tcggcgcgcg aagtgataat ttccgcttca
    25621 gtcggataat ccggcccctg cacgatatcc agcagctgat cgagcgtggt tttcggctgg
    25681 tcgattaatg cgattgccgc ctgagctact tcacgcaggt tatgcggtgg aatatcggtc
    25741 gccatgccga cggcaatacc ggtggtgccg ttaagcaaaa tgtttggcag acgagcaggc
    25801 agcattttcg gctcctgcaa agtgccgtcg aagtttggca cccagtcagc cgtcccctgc
    25861 cccagctcgc tcaatagcag ctcggaatat ttcgacaacc gggattcggt gtaacgcatt
    25921 gccgcgaacg atttcggatc gtccggcgcg ccccagttcc cctgaccatc aaccagcgga
    25981 taacggtaag agaacggctg cgccatcagg accatcgctt cataacaggc gctatcgccg
    26041 tgcggatggt atttacccag tacgtcaccg acggtacggg ccgatttttt aaatttggcg
    26101 ctggcattca ggcccagttc agacatcgca tacacaatgc ggcgctgaac aggtttaaga
    26161 ccatcgccaa taaacggcaa cgcacggtcc atgatcacgt acatggagta gtttaagtag
    26221 gcgttttccg taaattcatg tagcgcaagg cgctctgcca tatcgctcat taattctgat
    26281 tcctcaactt attcgccagc cttcaaaggg gcaatattgc cggagatact acattatctg
    26341 ccgcgtcgag tcacaggaga tcagcgtgaa tagcgccgcc ggacgtgctc cgacggcgat
    26401 aaaggcatca atgtgccttg atatacaact ctttgctgta gacttgatcg gcatagaggg
    26461 cagcacaagg ttgcgcccat gccacaccac tcggcatggg ggtccgctcc gggcgattcg
    26521 agaagttgag gttatgcgag tccagggaag cctaaagcat caaagtagtc gactgtaagt
    26581 atacggttca gcccagttta gctgtgtctc caccagtgcc ccgcgcctcc tgccataagt
    26641 gcggcttgct catggatacc cccgagacgg cttacggcgc accggataat aaattttgcg
    26701 tatacatggt attccttacc ggagaaatta taagcccaac aacaatacgc ctgtggcatt
    26761 gttcgctcaa acttatcgcg agtgatttgg ctcacactcc ggtaaagatt ccccatcacg
    26821 ccaacggcac ataaacatcc gtttccagtc gctgctctgt caccccttcg gcaagattgg
    26881 tgtagtgaaa cagaatcggt gctttacgca ttttctcgcc gcttgcaggc agccagtggc
    26941 gaataatgcc ccatatcgtg tgtgaaatat cgtccagctc gccaacgtgg cgggccacgg
    27001 cataacgtcc accggtaagt tcaccattgc tcacaccata acgattatcg ggaatcggtt
    27061 cgctaacgct gccgcagata tcaaagcgaa acgcttccgg tgcggtggtg gcagggtcgt
    27121 cccaggcgac gccaaaagtc tggctttggt taacaggaga aagccccgtc tctttacgcc
    27181 acatgataaa tttcgccaca ctgtagttga gcaactcagg gctacagcgg tgcggcaaca
    27241 tcgccacagg gattgatggg aaatcgataa tgtttacatc cagtgtcagg tttgtcattt
    27301 tgcctccctg cgaaattatt cccgggaacg cgtcccggga gcggtaacaa ttacggattt
    27361 actttgcgga tctgtttgac gtcaatttca acagaattcc agtctttatc gacttcaccc
    27421 tgaatctcaa ccgtatcttt cggcgtcacc gtcacaccgt tccagcgttt gtggtcgata
    27481 tcaacattga tagtaccgct ggcatcttta aacacgtaga gatcgtcaga gatgcgttca
    27541 acgatattgc cgcgcagggt tacccaggtg tcgtcacgca gggattttgc gctttctacg
    27601 gtcgttacgc tgccgttcgg cccctggaat cctccggcct gactttgcgt tgccgatggg
    27661 ccagaaaaac cgccctgctc tgctgccatc accggtgcgc tgcacagggc cattactgcg
    27721 attactgctg cgaatttttt catgtttatt actcccttta atgtctgttt ccgagcattt
    27781 aacaagatag tccttaacaa cttcttaagg gaaaaaaata aaatttagtg ctgtacagag
    27841 cgcgttacaa cacggtttac tggcagcaaa tacggttatc gcagggatga aaaaatgcga
    27901 attttactga tagaagatga catgctgatt ggcgacggca tcaaaacggg ccttagtaaa
    27961 atgggtttta gcgtcgactg gtttacacaa ggtcgtcagg gaaaagaggc gctttatagc
    28021 gcgccttatg atgcggtgat cctggattta accttaccag gcatggatgg tcgcgatatt
    28081 ttgcgcgaat ggcgagaaaa aggtcagcgt gagccggtac tgatcctgac cgcgcgcgat
    28141 gcgctggagg aacgtgtaga agggctgcgt ctgggagctg acgattatct gtgtaaacct
    28201 tttgcgttga tagaagtcgc cgccaggctg gaagctctga tgcgccgaac caacggccag
    28261 gccagcaacg agctgcgcca cggtaacgtc atgctcgacc ccggcaaacg tatcgccacg
    28321 ctggctggcg aacccttaac actgaaacca aaagaatttg ccctgctgga attactgatg
    28381 cgtaacgctg gtcgggtact gccgcgcaaa ctgattgaag agaaactgta tacctgggac
    28441 gaagaggtca ccagtaatgc cgttgaagtg catgtgcatc atctgcgacg caaactcggt
    28501 agtgatttta ttcgtaccgt gcatggtatt ggctacacat taggtgagaa atgaaattta
    28561 cccaacgtct tagtctgcgc gtcaggctga cgctaatctt tttaattctg gcctcggtga
    28621 cctggctgct ttccagcttt gtcgcctgga aacaaacaac ggataacgtc gatgaattgt
    28681 tcgacaccca actgatgctg tttgccaagc ggttaagtac gctcgatctc aacgaaatca
    28741 acgcggcgga tcgcatggca cagacgccaa ataaattaaa acacggtcat gttgatgacg
    28801 atgcgctgac ctttgccatc tttacccacg acggcagaat ggtccttaac gatggcgata
    28861 acggagaaga tattccttat agctatcaac gggaaggttt tgctgacggg caactggtgg
    28921 gcgataaaga tcaatggcgt tttgtctgga tgacctcacc tgatggcaaa tatcgcatcg
    28981 ttgttggtca ggaatgggaa taccgtgaag acatggcgct ggcgattgtt gccgggcaat
    29041 tgatcccgtg gctggtcgca ctgccgatta tgttaatcat catgatggta ctattgggtc
    29101 gtgaactcgc gccgctgaac aaactggcgc tggcgctgcg tatgcgtgac cctgactcgg
    29161 aaaaaccact aaacgcaact ggcgtaccca gcgaagtgcg tccgttggtt gagtcgctaa
    29221 atcaactgtt cgcccgcaca catgcgatga tggttcgtga acgacgcttt acctccgacg
    29281 cagctcacga actacgtagc ccgttaacgg cgctgaaagt gcaaaccgaa gttgcgcagc
    29341 tttctgacga tgatccgcaa gcgcggaaaa aagcgctgct tcaattacat tacgggatcg
    29401 atcgcgctac tcatctggtt gatcaactgc tcactctatc gcggctggac tcactggata
    29461 accttcagga cgtcgcggag atcccgcttg aagatctcct gcaatcgtcg gtgatggata
    29521 tttaccacac ggcgcagcag gcgaaaattg acgtgcgact gacactcaat gcccacggca
    29581 tcaaacgcac cggacaaccg ctattgctaa gtttgttggt gcgaaatttg ctggataacg
    29641 ccgtgcgcta cagtccacag ggcagcgtga tagacgtcac gctgaatgct gataatttca
    29701 tcgtgaggga taacggcccc ggtgtgacgc cagaggctct ggcgcgaatt ggcgaacgct
    29761 tctatcgccc ccccggacaa accgctaccg gcagtggact tgggctatcg attgtccagc
    29821 gaatcgccaa attgcatggc atgaatgttg agtttgggaa tgcggaacaa ggtggatttg
    29881 aggcgaaggt aagctggtaa cttctctgtc tatgagagcc gttaaaacga ctctcataga
    29941 ttttactaat agcaaaatat aaaccgtccc caaaaaagcc accaaccaca aaccagacaa
    30001 ataccgagcg aaaatacata tacccaaaac tcgaacattt cccgcataaa gagttttctt
    30061 aagataagaa taataagtgg cgtaagaaga aaaaatgctg caatcattgc ttgcaatata
    30121 cgatcttcgc ctgaaacgga aggtatactg caaatattgt ctcccgactc aagcatccag
    30181 tcatacttat tgaaagcaaa aaaa
//
LOCUS       DAZGXZ010000055        30149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000055 DAZGXZ010000000
VERSION     DAZGXZ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.055"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..89)
                     /locus_tag="ACHKJF_002536"
     CDS             complement(<1..89)
                     /locus_tag="ACHKJF_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0949876.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEF"
     gene            complement(421..561)
                     /locus_tag="ACHKJF_002537"
     CDS             complement(421..561)
                     /locus_tag="ACHKJF_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0949877.1"
                     /translation="MPQKTIIVSILCLTMLLAIWMIHRSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            complement(696..998)
                     /locus_tag="ACHKJF_002538"
     CDS             complement(696..998)
                     /locus_tag="ACHKJF_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0949878.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGMALKPVHYARVPFG"
     gene            complement(1043..2167)
                     /locus_tag="ACHKJF_002539"
     CDS             complement(1043..2167)
                     /locus_tag="ACHKJF_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0949879.1"
                     /translation="MVLLNGNTVYSPLKELVKMPDFSVSLDREQDKNTLQELNNRVIS
                     LSMTDNRGFEADRVTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAH
                     EGPPDRLTLSANSADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIE
                     IDHADQTEESDMSFLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSD
                     GDSHQFRIADRQAYTGVKAYWLDLNHGKKKAVNIKKRKTHAEKSSSREGSYMEGAEGN
                     VFVLRKTYPNEIAAQRAAAARWQQLQRGAAEFSITLARGRAELYPEMHGTVTGFKTEI
                     DNQDWLIAKVEHNIDGSGFTTRLELEARIPEWIAEKESNG"
     gene            2322..3509
                     /locus_tag="ACHKJF_002540"
     CDS             2322..3509
                     /locus_tag="ACHKJF_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0949880.1"
                     /translation="MAETRFHGVRVRENADLVTAINDIDSSVIGIVAVADDADTKTFP
                     LDKPVLLTRVMDVLGKAGKTGTLYKSLKAIADQVSTRVVVVRVAAAKEGDGQPTQTQL
                     VIGEVKNDGTYTGMQALLVAEQNEAVGVRPRILAVPSLDTKEVVAALVTVAKKLRAFV
                     YAGCPGCNTVQEAVTWRADLAHREVMLIWPDFIAYNPNSGQNEAFPASAYACGLRAKI
                     DHEQGWHKSLSNVAVNNVLGISVPVFWSLQAEDSDAETLNDKEITTLIKRNGFRFWGN
                     RTLDTTTYTFETYTRTAQSLADTIAESQFETVDKPLTPSNVKDVLSDIRAKLSALVTA
                     GKLIGAECWYDVVDNGTTDLRQGIVRIRYRYTPVPPLEDLELHQTFTDEFFDGAFASL
                     GGE"
     gene            3509..4018
                     /locus_tag="ACHKJF_002541"
     CDS             3509..4018
                     /locus_tag="ACHKJF_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0949881.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLTRKTESFRGAGMP
                     GSVSVDFGLDDGALDLSFAVGGADAHLFSRYAGSLDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSMKNTWYRLSVDDLEKIEFDVLNFIWRIDGKDVLP
                     DRTRSMLGF"
     gene            4066..4455
                     /locus_tag="ACHKJF_002542"
     CDS             4066..4455
                     /locus_tag="ACHKJF_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949882.1"
                     /translation="MDKETEMATGTAVSNGEASAVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMNTADF
                     TALSSAAVNFFAPSGSNAPAPENTAEP"
     gene            4476..4613
                     /locus_tag="ACHKJF_002543"
     CDS             4476..4613
                     /locus_tag="ACHKJF_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0949883.1"
                     /translation="MEDPVADIAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHD
                     E"
     gene            4603..7395
                     /locus_tag="ACHKJF_002544"
     CDS             4603..7395
                     /locus_tag="ACHKJF_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0949884.1"
                     /translation="MMNNLHIRVALAAADTLTQPLRRARETVGALSDSLRETQDSIRS
                     LERQSEAFAAAQTKFDEATRKIAAVQQQLNRLQQAEREGTVLSERQREMMASLTTRLE
                     RLNRVRDVHQRRLEDSRRELERHGIAAEGSSQTIQAAIRRTAEYNEALARQRQALAQV
                     TRAQQRYEQAQERLGRIRNAGAAALAAGAAGLYAGGRAVAPVMDLQRHGAVIAAQAGE
                     GASDAVGYTGVINHMVHTGITPDAQLAAEAVGAVRSTLGGLGAVSENELTRITRKMLD
                     MQAVMGGDIPQHIQAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYS
                     THFRNMGLTGSETMTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGL
                     DAARMSSAVASGGTAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPA
                     FLGALARVSDRMADVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRG
                     LVRMATAWGERLQAWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLR
                     LGLALLRAIPAGLSVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLVWRY
                     WDRVRAFVSGFIEGVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTR
                     DLLEQCTSAGQMFGSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDE
                     ARKTPVAWEWDPEQKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNV
                     LRHLKGIEGNTGGLLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALP
                     QEEIQVQQWPTARPAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAE
                     MNRMARAGTGSFRDRD"
     gene            7406..7897
                     /locus_tag="ACHKJF_002545"
     CDS             7406..7897
                     /locus_tag="ACHKJF_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0949885.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(7908..8495)
                     /locus_tag="ACHKJF_002546"
     CDS             complement(7908..8495)
                     /locus_tag="ACHKJF_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0949886.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINAWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(8507..8938)
                     /locus_tag="ACHKJF_002547"
                     /pseudo
     CDS             complement(8507..8938)
                     /locus_tag="ACHKJF_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(9245..9847)
                     /locus_tag="ACHKJF_002548"
                     /pseudo
     CDS             complement(9245..9847)
                     /locus_tag="ACHKJF_002548"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(9847..10470)
                     /locus_tag="ACHKJF_002549"
     CDS             complement(9847..10470)
                     /locus_tag="ACHKJF_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP0949887.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAMKMTYVARGVSRI
                     TGATLTGETIVVYPAAELTSPLSVSGDIHPGGVTYEGITVTIRPDKE"
     gene            complement(10463..11359)
                     /locus_tag="ACHKJF_002550"
     CDS             complement(10463..11359)
                     /locus_tag="ACHKJF_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0949888.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     AAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARDALDTWLKTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            complement(11346..11711)
                     /locus_tag="ACHKJF_002551"
     CDS             complement(11346..11711)
                     /locus_tag="ACHKJF_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP0949889.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDIAWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            complement(11708..12289)
                     /locus_tag="ACHKJF_002552"
     CDS             complement(11708..12289)
                     /locus_tag="ACHKJF_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0949890.1"
                     /translation="MNAEIMRLLENMVRTGVVSEVDLKNWRIRATSGGLTTDWLRWNC
                     TRAGAFKIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDG
                     AEIRYDAEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKARTFELTDGGTMKGDI
                     THSNGNLLSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            complement(12286..12927)
                     /locus_tag="ACHKJF_002553"
     CDS             complement(12286..12927)
                     /locus_tag="ACHKJF_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0949891.1"
                     /translation="MAPDDLHRLDGLFQEILSGLSAAGINKTARAVGQAVRAAQQKRI
                     RSQQSPDGNRWPARKKRVFRVQAGVVFIWNGTQVRRLKNWRTTTGRYGPMITGYDTER
                     RGIRSFHKADIEEYLEIDTRRRTQSAIKRPPMFEQLRTNRFLKVKTDPGGVSVGFEGR
                     AARIARVHQFGEVSLVSAGNAVRYPQRELLGFSEADRQKVTEIIINNLWRNTR"
     gene            complement(12914..13381)
                     /locus_tag="ACHKJF_002554"
     CDS             complement(12914..13381)
                     /locus_tag="ACHKJF_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0949892.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            complement(13397..13570)
                     /locus_tag="ACHKJF_002555"
     CDS             complement(13397..13570)
                     /locus_tag="ACHKJF_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0949893.1"
                     /translation="MLFIHQIMLYFCTAVCALYLISGGYSVIRNYFQRKIDERAAEKI
                     SQKSAQQNNQNMP"
     gene            complement(13717..15762)
                     /locus_tag="ACHKJF_002556"
     CDS             complement(13717..15762)
                     /locus_tag="ACHKJF_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0949894.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPEGEENPVPGTTDGYDTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSALTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAPAPFSVVAVPPATAGSDSTTTESPQPYTPAPQPG
                     AGTQSSPARTTAVAYRADAQEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQ
                     QTAWKIAHDVPASKAAELLQKGGEIAFRFKIPEGVQLAENRFAMALHWPVSQLPENVV
                     LEGSAGNDMLAAFFIQSDSANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSIS
                     VTPVINGQTQAAFNLAKCPAAGLDNDKLLITDITSNKDTYPVLIEYVTVDINNSNAA"
     gene            complement(15891..16316)
                     /locus_tag="ACHKJF_002557"
     CDS             complement(15891..16316)
                     /locus_tag="ACHKJF_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFP0949895.1"
                     /translation="MKLNIILCALLLTISLQAMVVIRAYRSGQESTRYEALEKAVKES
                     NTTVRELLKATAETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPADVA
                     DSLQQHASAAENRRHLRERAGKSDQTDTGTGAAATHDMG"
     gene            complement(16313..16855)
                     /locus_tag="ACHKJF_002558"
     CDS             complement(16313..16855)
                     /locus_tag="ACHKJF_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0949896.1"
                     /translation="MAGPKRYAVAAIVALGISLAPGELRTSREAQIKIATWEECRARP
                     YRDLVGVGTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQMPQSAFE
                     AMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQTRQWRLMCYRLTDFVNSGGTRTQG
                     LVNRRNDFKNWCLKDAGAQP"
     gene            complement(16842..17126)
                     /locus_tag="ACHKJF_002559"
     CDS             complement(16842..17126)
                     /locus_tag="ACHKJF_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0949897.1"
                     /translation="MFQRLCDHITFWASVTTAGIGVMTLSEKIAVAGFVLGTLSLLRA
                     WLHRRRMEIAQARRNELIEKILMQSEGRELNDAERRAMMLLKQDEHDGRT"
     gene            complement(17129..17329)
                     /locus_tag="ACHKJF_002560"
     CDS             complement(17129..17329)
                     /locus_tag="ACHKJF_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP0949898.1"
                     /translation="MKVMALDGDTVDLLCQRHYGTTQGVTEAVLAANPGISAVMFLHA
                     GQEVELPELQGQTRREMIQLWG"
     gene            complement(17329..17823)
                     /locus_tag="ACHKJF_002561"
     CDS             complement(17329..17823)
                     /locus_tag="ACHKJF_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0949899.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            complement(17928..18725)
                     /gene="gpM"
                     /locus_tag="ACHKJF_002562"
     CDS             complement(17928..18725)
                     /gene="gpM"
                     /locus_tag="ACHKJF_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFP0949900.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLPDFEGWIEGTLAGDSGRQDEVITTMMVWAIDCRDYPLAL
                     RIGEYVIRHGLALPDNFGRDAATVLAEEITDAVLTQATTDSEADLSVYTAMLDELNHL
                     VSEQDMPDQVRSKLHKARAFSRRASSSPDDLRLSLSLLREAMRINPASGVKREIATLE
                     RAVRKLADGNQVVPASTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            complement(18766..19866)
                     /locus_tag="ACHKJF_002563"
     CDS             complement(18766..19866)
                     /locus_tag="ACHKJF_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0949901.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEKVLIDTSGPIASTNATSDGVKRRNPASVADL
                     DARRYRCEQVNYDTFISYAQIDAWSSQKNFQQLLSAQITRQVALDRIMIGFNGESHAL
                     ISDRATNPLLQDVNPGWLKHIRDKAATRVVKGMTLTRRDDENKLVAKGDYGNPDAMVH
                     DIRSSVLDEWHKDAPDLVVLMGRDLFNTLRLPMINAISTTNPNTELVAGQLIVASRTV
                     GGLQTYLAPYFPKDAMLITSLKNLSIYFQKGSLRRFIREEPEYNRIATYQSMNDCYVV
                     EDYGKCALIEGITFAGEDPDNNAESGQPQPEA"
     gene            complement(19884..20720)
                     /locus_tag="ACHKJF_002564"
     CDS             complement(19884..20720)
                     /locus_tag="ACHKJF_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0949902.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GKKVYSSIEMLPELDAVKGPYIVGLAMTDTPASLGTERLEFAAQQRASIMQFSKCTTE
                     PPMFTVAMEAEMLTTTEQRTEESRQWFSRVMALISKTRTADGEQFSQLRDVVENIASD
                     HADLIDRFSTLEQKHALDKTALDELSHELAALKAQLRNQDADPQQRFTSAGGTDQTLA
                     EF"
     gene            20876..22609
                     /locus_tag="ACHKJF_002565"
     CDS             20876..22609
                     /locus_tag="ACHKJF_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0949903.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTRQLKKLNDGQGEQAVSGKKP
                     RRRKLKNHFTEEQIVALREKILPSLAWHQREWYDNRHHRNRMILKSRQIGATWYFARE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTGHLKFDEFFWVSNFVELRKVAGAMATLEGLTRTYFSTPSGET
                     HEAYQFWTGDRWNSEQPRSKRSEFDVSHAHLKGGVLFPDETWRQIVTIEDAVANGWRY
                     TNIGVIRGENTESEFRNLYMCEFVRDGEAAFSLSALTGCGVDGYDEWPDWKPFAARPM
                     GVREVWLGYDANGSSGKGDCGALSVCVPPLVAGGRFRTVETVRVQGMEFEQQAALIEE
                     YTQKYNVKHIGIDVTGGNGEGVYQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGR
                     WEYDRSERDLIQAFASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAE
                     REDNRGFAMEF"
     gene            22615..23658
                     /locus_tag="ACHKJF_002566"
     CDS             22615..23658
                     /locus_tag="ACHKJF_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0949904.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSAFTFDGPYEVSHMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALDWFVFGNAYLEVRRNRLGEPLLLRPALAKYTRRGSDLDTYWYLNDDGTEFSFRKG
                     TVCHVLNPDINQEIYGMPEYIGGLLSVSLSNSADTFRKLYYDNGSHAGCIIYVGTPQA
                     NAESVEAIKKTLTDSRGRGAFKNLFLHAPGGGKDGVQILPFQQITAKDEFLNIKGSAR
                     DDILAAHRVPPQLMGAMPDGNAAFGDVEKAARVFFINELQPVMEAMKHVNEWLGVEVM
                     RFNPYALLLDNAS"
     gene            complement(23724..24227)
                     /locus_tag="ACHKJF_002567"
     CDS             complement(23724..24227)
                     /locus_tag="ACHKJF_002567"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949905.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKMHIISKLQR
                     RTPSAFR"
     gene            24602..25309
                     /locus_tag="ACHKJF_002568"
     CDS             24602..25309
                     /locus_tag="ACHKJF_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949906.1"
                     /translation="MQTGLSQRRYRAEWGKSTLEKLCNFVRFCAVLILRFFVAGGDDI
                     HLSESVGDCRVRTVKNYSYPYLTFVTLSVLGGIFLFIWWRNDVYRITYLYSFTTNCLV
                     SVSVVLSVFVSLCLLKKNLVDRSKFTGVTSYLKFFSGMCLLTWMILFTLLVTLIYFLP
                     GEDSFYSAPYEYSRGSSKSCSGAFVDDPDLQKTIFICYPYGDYQDGNVIYVKKRVNAL
                     GAVVTYAYATSGRFRFD"
     gene            complement(25359..25676)
                     /locus_tag="ACHKJF_002569"
     CDS             complement(25359..25676)
                     /locus_tag="ACHKJF_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0949907.1"
                     /translation="MPTQVATEGRLAADPNTRRTTKGTNVTTAQLAVSLPCHGAEQPT
                     TMWLYLIAFGKQHEELAKHRKGDLILVHGNLQVNRYQDKYGQQKEGWQLLAQSIISSR
                     TGR"
     gene            complement(25709..26047)
                     /locus_tag="ACHKJF_002570"
     CDS             complement(25709..26047)
                     /locus_tag="ACHKJF_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0949908.1"
                     /translation="MKTPRMTGKVIILRYLETHARFSAPELVEKLGVKRTIINQAAGK
                     MVREGLLVVDGYTDKITHYRKLTEKERLELAQRAEWQQEPSVIEACKRSEIMKRILFI
                     YGAGEELPVI"
     gene            complement(26044..26253)
                     /locus_tag="ACHKJF_002571"
     CDS             complement(26044..26253)
                     /locus_tag="ACHKJF_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949909.1"
                     /translation="MNMTRNSKPKEHTMNDIRRISDKELTKMIESAQQLLSRRNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
     gene            complement(26263..26667)
                     /locus_tag="ACHKJF_002572"
     CDS             complement(26263..26667)
                     /locus_tag="ACHKJF_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949910.1"
                     /translation="MTRFHCVLQRHDFSTIMNARIPALTLSGVSVRPQPGDELEITIA
                     SPDRSTDTVMTDILTARINTDRSAFIVAVRPRRAISEPEPLTPVGKIMQAYHEKQTRK
                     LNEELARTLAVVNRKAAIVSYVHSAASRQSRR"
     gene            complement(26664..29177)
                     /locus_tag="ACHKJF_002573"
     CDS             complement(26664..29177)
                     /locus_tag="ACHKJF_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0949911.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVAFLTTTDEGKRINSHLHHNEERRIRLEKLAKHKRENPPTVVSEAMAE
                     LRALPSFLGNVLIRDLARLNRKQQSARNEGVKNNNTHVADNFVRRGLRTRLKRIEQVN
                     ECFATTAFKATAARHRLDELLMLPQLNRDQIQRLATLTAAAFSTELERICDEVTERTG
                     KGDDNLFTWLLTYQQLARMALKLGVTPPYWPSLEIRRDRRTAPDPELVPGAVMRITCA
                     TWWNNQLRHLADLWREELLRAAGRVSRKASPYISHESLQEFREKRQRTRDFLKSWDIE
                     NEDGERLSLEDVYWSGLGNPRNRRNEMMACVRGMEQVAESRGDSAFFVTVTCPSRFHS
                     VNEDGSLNPKYNGATVRDASDYLVYDVFAAARKKLNKEGLSWYGVRTVEPHHDGTPHW
                     HMLVFTAPDNEERIIEIMRDAAIKSDRAELGNDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASTFHDNDPKTGKPYADKESGKTMAETVENAIAWASLHRIRQFQFFGIPPRQV
                     WRELRRLAGQMERNPASPKRLDHDDIDAILAAADVGCFATYINRQGGVLIPRNLYLIR
                     TAYETADEANDYGEFPQRIYGICAPSLGERYTICTHPDEWKLVRKEAKADNSTDEDFD
                     VGGGFAAPWTRGNNCPHDEKTDTGEGKTPPPETRKEQKLTIPEGDLGEWLRSLTPTER
                     KQLTRQLKDAPSDTKTVKTGGSTTPDDVIHVRTALPLTDVTTTVIKSALSTTGEELHS
                     GAALSVHRGARVRLPDGRIVHWDEKSRSLVAADQPEVNP"
     gene            complement(29174..29590)
                     /locus_tag="ACHKJF_002574"
     CDS             complement(29174..29590)
                     /locus_tag="ACHKJF_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949912.1"
                     /translation="MKSVKGLSDIQDFFNWMWAVDPLLASRLNNWHDYYRRACKQAAR
                     SVTTYGDFSFTVDGRYRVDVKGDGDGLGLFHINGTDSTAISWSSSEMALILEMTTHSV
                     AVKGIYTAEGFIGEYQRLLVLYRAAFQVATAGECDA"
     gene            complement(29659..29880)
                     /locus_tag="ACHKJF_002575"
     CDS             complement(29659..29880)
                     /locus_tag="ACHKJF_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0949913.1"
                     /translation="MANRKQQRASAERLHIRSEINRRLFRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYLAEDLAELQSLINQHR"
BASE COUNT         5919 a         8015 c         8527 g         7688 t
ORIGIN      
        1 ctgaactcac acggcgaacg gtgtatcatc cagactgtca acaacatggt taagcataaa
       61 atgctcacga tgatcgtttt ttgcggcata gcgcttgctc ctttttcaaa gaggcgctaa
      121 ccttccactt gcgaaggtta gatggttagg gcctcgggtt aaacaaatgt ttgactcggg
      181 gccttttcac atccggcctt caggtaatcc ttccagccgt cagccgaaag gcacccacgt
      241 gtaatctact gatttccctt tgtgcgcgca atgctcagtg ttgaaactgt ttagggggag
      301 ggcggcaaaa aagcccgcat attgcgggca aatacgtctg taatccaatg gagaaagggg
      361 tgccatatcg actgaacccg acaaccggat agcgggggct ttcacccccg ctgcggtttc
      421 ttactgttta cactgcaaga acgccgcaaa ttctgaccac atgatgttaa gtctgaactc
      481 acacggcgaa cggtgtatca tccagatggc caacaacatg gttaagcata aaatgctcac
      541 gatgatcgtt ttttgcggca tagcgcttga ctccttttac agggaggcgc taaccttcca
      601 cttgtctagg gtaggaatgg ttagggcctc gggttaaaca atatgtttga ctcggggcct
      661 ttccacatcc ggccttcagg ttttccttcc agccgtcagc cgaaaggcac ccgcgcgtaa
      721 tgtactggtt ttaaagccat accgcaatcc tccagttgta gcgttaactg actgcgtccc
      781 tgatgggatt tgcgcattgt ctcccgtgga atatctttat cgtggaagat caactgaggc
      841 tggccaataa ttctgtgcac actttccatt gttgaaaatg tggcgctgca ctcaaggtta
      901 ttgcactgat agtatttgta gcggatggtg ccggtatcat tggcggcttt actggtacgg
      961 atccgggcag atgcgccgca gaatggacaa tgaaacattg atataccccc ctggctgaac
     1021 tggggttgaa ctcgtcgcca ttctaaccat tgctttcctt ttcggctatc cactcgggta
     1081 ttcttgcctc cagttcaagc cttgtggtga agccgcttcc gtcgatattg tgttccactt
     1141 ttgcaatgag ccagtcctga ttgtctattt cggttttaaa gccggttacc gtgccatgca
     1201 tttcagggta aagctccgct ctgccccggg ccagcgtgat ggagaattca gccgcaccgc
     1261 gctgtaattg ctgccacctg gcggcagctg cgcgctgtgc agcaatttca tttgggtagg
     1321 tttttctcag aacgaacacg ttaccttctg ccccctccat gtagctgcct tcccggctgc
     1381 tgctcttttc agcgtgagtt tttcgttttt ttatgttgac ggctttcttt ttgccatgat
     1441 tcaaatccag ccagtacgcc ttcacccctg tgtatgcctg gcgatcggct attctgaact
     1501 ggtgactgtc tccgtcactg cgtgccagtg caaatgaggg gagtgggcgc ccgtcagctg
     1561 tcacgccgcc acccggcaga atgaacagca gacagccatt tttgacggta gtgatggctc
     1621 ccagcatatc agccatgcgg gtaaggaatg acatgtcgct ttcttccgtc tggtcggcat
     1681 gatcgatttc aatatccata agcatatcgc ttatctgggc tttcagtccg taacggtggg
     1741 caatagcgga aaccacgcgt tcaacagtca cgtcgtgcca ggatacttcc cgtttgacgt
     1801 taaattcctc cctgaagtct gcgctgtttg cactcagtgt cagccgatcc ggaggtcctt
     1861 catgagcgac ctcatcaaca atgtaggtgc ctttatcaac cagaggctct cccttccatc
     1921 ccagcatgac tctgatgtgt gttccccgcc cggggagagc cagctgcccg tcggcatcat
     1981 ccagcacaag agtgaccctg tctgcttcaa acccccggtt gtctgtcatg gaaagcgata
     2041 tcacccggtt attcagttcc tgaagagtgt ttttatcctg ctccctgtcc agtgaaacgc
     2101 tgaaatcagg cattttcacc agttctttca ggggggaata aaccgtgtta ccgttcagca
     2161 ataccataat tcctccgttt ttcccgccac agttgcatac cagaaacacc accggaatga
     2221 gtttgggttg tatgtgtatt gcgagaaacc gcagttcgtg aaaaagctcc ccgtgttcgt
     2281 aaagatctct gctgaactca caccaatgca gggtgaataa tatggctgaa acccgtttcc
     2341 atggcgtgcg ggtccgggag aatgcggatc tggtcacggc cattaatgat attgactcca
     2401 gtgtgattgg tatcgtggct gttgctgatg atgctgatac aaaaacattt ccgctggata
     2461 agccggtgct cctgacgcgt gtcatggatg tgctgggtaa ggccggaaaa accggcacgc
     2521 tgtataaaag tctgaaagct attgccgatc aggtcagcac cagagttgtt gtggtgcggg
     2581 ttgctgctgc gaaggagggg gatggtcagc caacacaaac gcagcttgtt attggtgagg
     2641 tcaaaaatga cggaacctat acaggcatgc aggcgctcct ggtggctgaa cagaatgagg
     2701 ctgttggtgt tcgtcccagg atcctggccg tgccatcgct tgacaccaaa gaggtggtgg
     2761 cggcactggt aaccgtcgcg aaaaagctgc gtgcctttgt gtatgcgggc tgtcccggct
     2821 gtaataccgt gcaggaggct gtcacctggc gcgccgatct tgctcaccgt gaagttatgc
     2881 tgatctggcc tgattttatc gcgtacaacc ctaacagtgg ccagaatgag gcttttcctg
     2941 catctgcgta tgcgtgcggg ttgcgtgcga agattgacca cgagcagggc tggcataaat
     3001 ccctgtccaa tgtggcagtg aataatgtcc tgggtatttc tgtgccggtg ttctggtcgc
     3061 tccaggcaga ggacagtgac gcggaaaccc tcaatgacaa ggagatcacc acactcatta
     3121 aacgtaacgg cttccgtttc tggggtaacc ggactctcga caccactacc tacacctttg
     3181 agacctacac gcgtacggcg cagtcgctgg cggataccat cgccgaatcg cagtttgaaa
     3241 ccgtggacaa gccgctgacg ccgtcgaacg tgaaggatgt gttaagcgat atccgggcaa
     3301 aactgagtgc ccttgtgaca gcgggtaaac ttatcggggc agagtgctgg tatgacgtgg
     3361 tggataacgg cacaacggat ctgcgtcagg ggattgtgcg gatccgctac agatatacgc
     3421 cggtaccacc gcttgaagat ctggagcttc accagacgtt tactgatgag ttctttgacg
     3481 gtgcatttgc atcactggga ggtgagtaat ggcggtacca aacaaacttc gcgttttcac
     3541 ggtgtacatc gatggcgaaa acaagctggg gcgggtgacg tccttcacgc cgcctaaact
     3601 gacccgaaaa acggagtcct tccgtggtgc cgggatgccc ggcagtgttt ccgtcgactt
     3661 tggtcttgat gatggtgcac tggatctctc ttttgcggta ggcggcgcag atgcccacct
     3721 tttctcccgt tatgccgggt cacttgatga agtccgtctt cgcttttccg gtgagtacta
     3781 ttcggatgcg gatatcagat atgtggatat tgaggtccgc gggcgcatca ctgaaatcga
     3841 tatgggggag gcgaagcagg gtgaagacac tgagcacagt tattcgatga aaaacacctg
     3901 gtacagactg agtgtggacg atctggaaaa aatcgaattt gatgtgctga attttatctg
     3961 gcgcattgac ggtaaggatg tgctgccgga tcgcacccgc tcgatgctgg gcttctgatg
     4021 atggtttaac agagaacggc ggggatcccg ccgtggagac tgaaaatgga taaagaaacg
     4081 gaaatggcca ctggcactgc cgtcagtaac ggggaggctt ccgcggtggc agttgccgct
     4141 acggctgcac ctaagaacgt ggtgaagctg acggtgcctg ttgtgcgcgg agatgtgtcg
     4201 tacagtgaaa ttaaaatcgg accgctggcg aatcaggccg gatcgctgcg tgggctgtct
     4261 ttgcttgcgc tttacaacat gaactgtgat gccatggtca cctggcttgc gcgtgtgaca
     4321 gagccaaaac tcaaggaggc cgaaatgtat gccatgaata cggcggattt tacggcgctg
     4381 agctcggcgg cggttaattt tttcgcaccc tcggggtcga atgcaccggc accggagaac
     4441 acggcagagc cgtaacctgt ctttgctttg actgcattga agatcccgtt gcggatattg
     4501 ctgttatttt taactggccg cccgcagaga tatttgcaat gacgcctgca gaggtggtcg
     4561 cctggcgtga gcgggcggct gccagaagcg gaagtcacaa tcatgatgaa taatctgcat
     4621 atcagggttg ctctggcggc ggcagacacc ctgacacaac ctctcaggcg cgcccgcgaa
     4681 accgtgggcg cgctttctga ttccctccgt gaaactcagg actccatccg ctcgcttgaa
     4741 cggcagagtg aagcctttgc tgcggcacag actaaatttg acgaagccac ccggaaaatt
     4801 gccgctgttc agcagcaact gaaccgtctg cagcaggcgg agcgtgaggg taccgtgctc
     4861 agtgaacggc agcgtgaaat gatggcttcg ctgaccaccc ggcttgaacg tctgaaccgg
     4921 gtgagggatg tacatcagcg acgcctggag gattcacgcc gggagctgga gagacacggt
     4981 attgccgcag agggcagcag tcagaccata caggcagcca ttcgccggac ggcagagtat
     5041 aacgaagcgc tggcgcgaca gcggcaggca ctcgcacagg tgacccgtgc acagcaacgc
     5101 tatgaacagg cgcaggagcg actcgggcgg atacgtaatg ccggtgcggc ggcactggct
     5161 gccggtgcag caggacttta cgctggcggg cgtgccgttg cgccggtgat ggatctccag
     5221 cgtcatggtg ccgtcattgc ggctcaggca ggagagggcg cgtcagatgc cgtggggtat
     5281 accggggtta taaaccacat ggttcatacc ggtatcacac cggatgcaca actggcggct
     5341 gaagctgtgg gtgctgtccg ctccacgctg ggtgggctgg gggcggttag tgagaatgaa
     5401 ctgacccgaa tcacccgaaa aatgctggat atgcaggccg tgatgggggg cgatatcccg
     5461 cagcacattc aggcggctgc catcatgatc aaaaacggtc ttgcacgcag cagtgacgag
     5521 gcggttgatc tgatggtggc gggcatgcag cgcatggggc cggttatgcg cgatgagctg
     5581 cctgagatcc ttcacgaata ttcgacccat tttcgtaaca tggggctgac agggtcggaa
     5641 accatgacgc tccttgcgga tatggcgcag caggggaagt ttgcgctgga taaaaccggc
     5701 gatgccatca aggagttcag tattcgcggc tccgatatgt caaaggcgag tgctgaagcg
     5761 tatgcgctta tcgggctgga tgcagccagg atgtcatcgg ctgtagccag tggtggaacg
     5821 gctgcacggg ctgcgctgca gaaaacagcc tcgggtctgt tgcagatcgt cgatccggct
     5881 gagcgggcca atgcggccat cgcacttttt ggtacaccgg ttgaagatct gtctgtcgat
     5941 caaatccctg cctttcttgg cgcgctggcg cgggtgtctg accgtatggc tgacgtgtct
     6001 ggtgtggcgg accggatggg gcaaaccctg cgtgacaatc tgagtggtga tgtggcgcgg
     6061 cttaccggga cgttcagcgc gctgagaagt gggattatcg gggagattac ggacgatctg
     6121 cgtgggcttg tccggatggc cacagcgtgg ggtgagcgtc tgcaggcgtg ggtgaatgcg
     6181 catccggaac tggtgcgaat gattgcgctg acgacgggag cggtgctggc gttaaccaca
     6241 gcgatggggg cgttgtccgt tgcggcctgg atggttgtcg caccgttact gaaactgcgt
     6301 ctggggcttg ccctgcttcg cgcgatcccg gccggactct ccgttgccgg tacgctgttt
     6361 ggcggactga cgggtgtgat ttcagccgtg ggggctgcgc ttggggcgct gacctggccg
     6421 gttgtggtgg cggtgggagc cctggtcggt ggtgctctgc tggtctggcg ctactgggat
     6481 cgggtcaggg cgtttgtgag cggatttatt gaaggggtac agtcagcact ggcgcccttc
     6541 atggcgacag cctcccgttt tgcgccgctg ctggatctgg tttcaggggc actgtcccgt
     6601 attctgggat ggtttaaatc cctgttaacg ccggtctcct ccacgcgtga tttgcttgag
     6661 cagtgcacct ctgccgggca gatgttcggg agtactctgg ccggtgcgtt agccacggtt
     6721 ttttcgcccc tgaaagctct gattgacggt ctggcgtggg tgctggaaaa actcggtgtt
     6781 ctgcccggtg aagctgagcg ggttaagcga aaactggatg aggcccgtaa aacgccggtg
     6841 gcgtgggagt gggatccgga acagaaaaaa atggttcaga aggcgtggca ctggtcgcct
     6901 cctggtgggg ccagtgtgcc ttccggcgcc tccggtacac agcctccgcc ggtggcgctc
     6961 cctgcgggga tagcgccgga cggtaaccgt aacgttctcc gtcacctgaa aggcattgag
     7021 gggaataccg gtggtctgct ggctgaaaac cgtaaacgta tcggacccgg agatattgtg
     7081 tttaaacatc ttccacgggc gctggcggtc cgggggcagt ggcatgaacc tgtcgttgcg
     7141 ccgtcttccg gcggaatgcg cgcgctgcca caggaggaga ttcaggtgca gcaatggccg
     7201 acggcacgcc cggcgccacg aatgcaccag caggaaacct tcgccagcgc ctttcagggg
     7261 gagatacatg tgcatcttca cggggtgagt tcgcataatc cccgggaact ggcgcgcatg
     7321 gttggtgacg ctgtcagggc ggaaatgaac agaatggcac gtgccggaac cggcagcttc
     7381 cgcgatcgcg actgaggtga aaaagatgat gatgatttat gggatgtttg tgtttcagtt
     7441 aagtaccctg ccgcatcagc aggtggaaca gagtaaacgc tggcgacacg tcagtaatga
     7501 acgggttaac cgttcatcct cctggcagta tattggctct ggtgaagatc acatcacgat
     7561 gtccggtgtg ctctgtccgg aaatcaccgg cggggaggtg tcactgactg ccctgaacac
     7621 cctggcctac acaggattac cctggccact gattgatggt gtcggacaga tttacgggat
     7681 gtatgtcatt acggaaatca ggaccacccg tcaggagctg gatcgctatg gcatggcccg
     7741 caaaattgag ttcactgtca gctttcagcg atgcaatgaa gatctgcgtg agcgtcttca
     7801 gtcgggctcg gtgggtgatc tgatggcagg attaaaaaac agcgcgtcgt cggccctgtc
     7861 gtcggcgtcc tctgcgctgt ctggtctcat gttgtgaagg tcactgttca ggcttcggct
     7921 tcttttcccc agccaacctt atagtccaga atcgccgccg tatcgttcag ggcggcgatc
     7981 tcttttttca tgttacgctg gcgaatgtga atttccatcc ctttggcaaa cagggctttt
     8041 gcggctgcat cgctgagggc aatcagctca tcagcagaaa caggaacatc gttgttctgt
     8101 gcatcagtcc agaaaaaatc cagcggcagg atccccgctt ttgcggcagc aacggaaggc
     8161 tcaaggcggg cctgcgttga cttcccgtag tcccatttcc tgccgttatg ttcaaaggtg
     8221 taactcgccg cttccatcgc attacgccag gcgttgattt cgtcgttttt caggcgtctg
     8281 gcgttttccc tgtcagtgtc gttttcctgg tattcagata cagggccgtg atttcctgct
     8341 ttcagttcgc cccagagttt tttgccgtgt ggcgagttgt catattcggt tgccgtatag
     8401 ggcagccagc tctcctcccc ttcaaactgg atctcacagt cgatagtgcc gtcacggtta
     8461 taagcggcat ttcttgctga tttgattgtg tgcatgagtc tctccgtcag gcaatgcgct
     8521 caaatattcc gattcctgcg cctgcataaa tgtcagcgca ggcccgccac gtgcccggtg
     8581 gcctgcggtt tgtgttccag ttttgcatgg attcacgggg gccgaagttg ccttcggaat
     8641 gagtcaggct tatcagtctg agtttactgc ccgggcgggt gtttccggcc gttatatcaa
     8701 ttttggctgg ccactcccct tcggcatccg ttgccgccag aataaagctg cccacatccc
     8761 agagtgtccg ctgtgttttt gcctgtgcag cctccagtgc ttgccgtgct ttctgcccgg
     8821 cgttttctgc tgccgtcatg gccgtcttca cagcttttgg tgtggcagcc agcacttcac
     8881 tgtcactgtc cgttgcgctg cttagctgaa caatacctcg ctctgtcagg gaggcgtcgc
     8941 cggtctgcgt gagggggagt atggcaacct ccttccattc agaccatggc ccgttgccgg
     9001 tgaagttacc tgtcactgtc cggataaatt ttcgtggtgg attccaggcg gtgtattcct
     9061 gctggcagaa tccccatgcg cccatcctga tggataatga tccggctgat gttaccgggt
     9121 agtgatgtgc tgtagtggcg ccgttgttgt tgggctgata gtagtcacca tgatgttcag
     9181 gtctccccag tgtattcaga tccacggcga ggggggctgt tggtcttgtt actgtcagcg
     9241 ccttaagtgc ttttattgct ttgggtgtgg ctgcccgggt ttcatcatca ctgtcggttt
     9301 cactactgag ctgagtaaat cctttactgg tggttgtggc atccgggtgt ttaccgtttt
     9361 gttcatgctg ctttatcatt tctgccacgc cagcctgtgt tgccatggtg gttgatgagt
     9421 cggctgtcag atttacagag gcttcgttgc tcagcagaat caccatgcgg aatacctgtt
     9481 ctctgcctga gccctcatca agctgcggtt tgtaaccttc cgcaacatta cccacggcga
     9541 tgagtgtgcc tttgctgtca aacagaccga tttcgcggat ccagaatcct ccttcattcg
     9601 caggaatgat cacttcggcg gttaaaaaac tggtgttttc cgcgtcacgg ctgaccctgc
     9661 tgacctcgcc ccgccagacc tctttaacga gagcggtttg cgtggccagt ggttctgtgg
     9721 gtttaccgtt accgtctccg acggcgatat gtgtcagcgt gatcttttct ccatccggta
     9781 acaccgcagc tgcgagggct gctgtgccgg catttgtgat aatggttttg tattttgcgg
     9841 ccatcattac tccttgtccg gtctgatggt tactgtgatg ccttcatagg tgactccgcc
     9901 cgggtggatg tcaccactga ctgacagcgg gcttgtcagt tctgctgccg gatataccac
     9961 gatggtttcc ccggtcagcg tggccccggt tatgcgggat acaccccgtg caacgtaggt
    10021 cattttcatg gcggcaaggt gtcggcttac cgggcgaacg gtggtaatca ttcgctccat
    10081 ctcctgccgg gtttcctctg tcagtccgcg ctccgatatg tcaatatcca gcctgaatga
    10141 ccccggcgga tcgttgttct cccaccattc tgtaatactc agtatcgcgc cgaagggctc
    10201 cacagcccga cgcatggcgc tgatggtgcc tttgtggcgg tgaatatgcc acgagtcacg
    10261 gatgatctga cgacgtgcct cttcactcca tgtttcatcc cagcggtcaa cggaaagcgc
    10321 ccaggcaagc catggcagca gatgggctgg gcaggtgtcg gggttccaga gggtgttgat
    10381 atggatcggc agctctgaca ggcgctgagc gccctgttcc gaatgatgca ggaaggaggg
    10441 cgatgatggc ggcagcagtc ggttattcat cggtgccacc tgcagagatg ttccgggatg
    10501 tgcagcgggc tgactgggtg ttactgatca ccacattctg cgctggtttc ttcagctcga
    10561 ctcgctgaac gcccggtacg tgtagcgttg ccataatggc agagcgggcc acatcacgcc
    10621 ctatggcatt ctgagtcttc agccatgtgt caagtgcatc tctggcggcg gcaagaatgg
    10681 gttcagattc cgggccagga taaaaataga gtacggcctc aatatcgtaa ctgacgatgg
    10741 tcgcactctg taccgtgagt ctgtcggcca gggggcgcac agtttcatca ttgagagccc
    10801 ggctgacggt cgcgattaat tccggtgacg ctgtgccgtc tccttccgtt gagagaatgg
    10861 agacaatgac cgtcgcaggt gacggactgg ttgcccgtgc atcggcaaca agaccgctgg
    10921 cactgcgggc aaaatattca tatgcagcag aggggcctgc gacgctgaaa ccttcaaatc
    10981 cggattgtgc ccgcagacgg aacgcgctgt cgctctccat gatggcagct tctgtatcag
    11041 tagcaggttt cagttcaagg cgtttcgcat tcacgatagc ggcgagatta tccaggtctg
    11101 tggatacggc atggctgagc atgcaccccg ccgctgcatc gttgatccgc tgacggagca
    11161 gcatttcgcg aaaggcgatg gcctgagcaa tgatgttgag tggctctgat tccagcgcaa
    11221 tggctgcgct gacggcactg cgcatgtttt cagggaaggc atcaatcata aaggccttca
    11281 cgtctttcag tatgctctca taatccagtg attcaatgat ctgcggacgt ggtaactgag
    11341 acaggtcaat tgtcggcatt actccccctc agcggaatgc tcatctgtac gggctgcatt
    11401 gtgcgggtga gcacgcctga gatggccagc agagcgcgtc cgtcaggcag ccaggcgata
    11461 tccacccggt taatggcgat ccggggctcc cactgtgcga gcgccataac caccgcgctc
    11521 atgcactgca gacgcgtcac gtcgttttgt ggcgcatcga gcagttcagg taacaggctg
    11581 ccatactctc ttcgcatgat gcgactgccg cgcggggtgg tcagaatatt gtttacagag
    11641 tggcggaggt gttctgtgtc actgatgcat ccgctaccat gcgggttcat gcctgtgcat
    11701 accggtgtca tttcggcccc cctgtcatgc ttccgccgga ctgaacaccg ttatgaacgt
    11761 gcgtatgcac ggtaacacca tttgagagta agtttccgtt actgtgtgtg atatcccctt
    11821 tcatggtgcc accgtcagtc agttcaaacg tcctggcctt cagatgtttt gtgcactcta
    11881 cggccggtgt gtccagggta atacgtgttt ccgcagagat ctctgcggtt ttgattcctt
    11941 tcaccgtcag cgcgcctttt tctgcgtcat agcgtatctc ggcaccgtca ggtgcaagga
    12001 gtatcacttc attctgtttt gtgccgggcg ccgggtgttc ttcgctgtag agactgccag
    12061 ccacaacggc catttcagga ctgccgcccg ggcagagcaa cagcacctgt tcaccggggg
    12121 aggggggcag ccagatttta aacgccccgg cgcgtgtgca gttccagcgg agccagtccg
    12181 ttgtcagtcc gccgctggta gcgcgaatgc gccagttttt cagatccacc tccgaaacga
    12241 cgccggtgcg gaccatgttt tccagcagtc gcatgatttc agcattcaac gggtgtttct
    12301 ccacaggttg ttgataatga tttccgtcac tttctgtctg tcggcttcac tgaatcccag
    12361 cagttcgcgt tgcggatagc ggacggcatt accggcagag accagtgaca cttcgccgaa
    12421 ctgatgaacg cgggcaatgc gggctgcgcg gccttcaaat cccacgctga cgccgccggg
    12481 atcggttttt accttcagaa agcggtttgt ccgaagctgc tcaaacatgg gtggacgctt
    12541 aatggcgctc tgcgttcgcc tgcgggtgtc aatttccagg tattcttcaa tatcggcttt
    12601 gtggaacgag cgtattcccc ggcgctccgt gtcgtaaccg gtgatcatgg ggccgtaccg
    12661 gccggttgtt gtccgccagt ttttcagtcg tctcacctga gtgccgttcc agatgaacac
    12721 cacacctgcc tggacacgga atacacgttt tttacgggct ggccaccggt taccgtccgg
    12781 tgattgttgc gaacgtatac gcttttgttg cgcggcgcgg acagcctgac cgacagcacg
    12841 tgctgttttg tttataccgg cagcggacag accactgagg atctcctgaa agagaccgtc
    12901 cagtctgtgc aggtcatccg gagccatcat tctgacccca tgtgacatct tcgagggtga
    12961 cgcgccatgc ttccagggcc cggctgacag gcggctctcc gatgtgttct gcgtgaacag
    13021 tgccatcatc attacggcgg ataatcaccc gctcccatac cgggatttcc agcaggatat
    13081 cggtgctgtc gtcgtcattg atggcaacag aaaatttgat atccctgttt ttctccggat
    13141 taagcaggag atcaggctga ttgcggtaca gccagcgcat cagcggcacc atcagttcat
    13201 cgagatcctg cgtgaagtcc atgacaaaga tcccaagcgt gtaacggtaa agatatgagg
    13261 gggattcgcc ggtcgtctca atgcctccgt tattcacata cacagtgaat ttctccgggt
    13321 tggcgcgaca ccatgggcag gcgtcagtca tggcgtcgcg cagggtgtta attttcagca
    13381 tgccccctcc tgtgtgttat ggcatgttct ggttgttctg ttgtgcagac ttctgactga
    13441 ttttttcagc ggcgcgctcg tcaatttttc gctgaaaata atttctgata acactgtaac
    13501 cgccgctgat cagatacagc gcgcagacgg cagtacagaa atacagcatg atctgatgaa
    13561 tgaataacat aatcacctca cacaaatgat tgacataacg atgtctggcg ttttaaaaaa
    13621 aatgaataac aataataaac actctccata attcctcttg cgctcccgtc aggtattttt
    13681 tcctctcagt cttgcctgcc gggagcctct tttttcttat gcggcgttgc tgttattgat
    13741 atccaccgtc acatactcaa tcagcacggg gtaggtgtcc ttgttactgg tgatatccgt
    13801 gatcagcagt ttgtcgttat ccagtcctgc cgccgggcat ttcgccagat taaaggccgc
    13861 ctgggtctga ccgttgatga ccggtgtcac gctgatgctg ttatttccgg caaaccggaa
    13921 caccacgttg tgccagttat gattaaatgc cccgaaggtt cccagttttg cattctgttg
    13981 tttgtgctgc atcagattca ggtttgcgct gtcgctctgg atgaagaagg cggccagcat
    14041 atcgtttcct gcgctgcctt ccagaacaac gttttcaggt agctgagaaa ccggccagtg
    14101 cagagccatg gcgaagcggt tttctgccag ctgaacacct tccgggattt tgaaacgaaa
    14161 ggcaatttcc ccgcctttct gaagtaattc agctgcttta cttgcaggca catcgtgggc
    14221 gattttccag gctgtttgtt gcccctggac tttttccact cgcatggctt taccgctctc
    14281 agcagcagag tcatttacga cggaggcttt tccatctgcc cctgtccagc cctgttcttt
    14341 cagtttccct tcctgtgcgt ctgcgcgata agcgacggcc gttgttcttg ccggtgatga
    14401 ctgcgtacct gcgcccggct gaggtgcagg tgtgtagggc tgcgggcttt ccgtggtggt
    14461 gctgtctgaa ccggccgtgg cgggaggtac tgcgaccacg ctgaacggtg cgggtgcacc
    14521 tttcaggaag gcgacggtct tcccggcatg catcaggatc gccgttgcca ggcggtcaga
    14581 aataatgccg cggcgggccc atgaactgaa atgcgtggcg cgatctgaag aggtccagtt
    14641 tccgttacta cgggatgcag caccgtaata accggcttct gccaggtcag gatcttcggc
    14701 aggcaggttt gttggcgtgt tagcaccttt ctcatcggtc atcaggggca caaaataaat
    14761 tttttgcgct tctttgttct gatatgcacc gtaaaccacc gggtactgag aggtatattg
    14821 ttgtttccag atataggtgg tatcgccgca aatccaggga acaagtgatg cgttgcctcc
    14881 ggtgcactgt gcagcatatg cgctgagatc ggcgcggaac tgattcacca tgttcaggaa
    14941 taattgcggg tgctgtgcgt atgttgctga tttcagatca aactctccca gcatccagac
    15001 cacggataac aggacgtttt tggggttttt atccagcgcg gcttttgtac gggtaagcag
    15061 atcgttatac agcggtttgc cgacgcccca cagggatgat tcggccgttg ctcctgaaga
    15121 ttcactgaat gccccctcag cgccggaagt aagcgctgac cctccacgac agcaaggtac
    15181 caggaggatc cctgcatttt tcgggataaa cgccagtaat tttttggcaa tatgtaaacc
    15241 ctgtccgacg cagccatact gaccttttga cagatcggcg ttcggatgat taaggctgct
    15301 catatcctgt acatcatgga gacagtgatc tgccggaata atatcgttat aggtacatgc
    15361 agcgccgccc ggtgttaccg tactgcgacg cgctaactgt ttaattcgtg gatcaggact
    15421 gtcgaatgta tcgggtaatg gcagcccctc accgtatgcc atgccattgg actgaccagc
    15481 cagtggcacc acataataaa actctggctc agttgtgtgt ccagtgtcgt atccgtcggt
    15541 agtgcccggt acggggtttt cctcgccttc tggtaccgga atggctgatg gcaggtctct
    15601 gtcagtgacc agtgcctgca tcaggtaaaa tggtgctccc ggctctgatg ctgagacagg
    15661 tcccccaaga agtccccatc cttccctgag catgggcgca agtttttcgg caagttcctt
    15721 gttcgtcatt gccgtcacaa gctgataatg tttcggtacc atagtgactc cgttattttt
    15781 catgagtgtt cattcgtgct gtccgctgcc gctccagccc ggcaatggcg gctttatcgg
    15841 cattacaact gtccagggca tctaccagtc ggtctcccca gtcagccaca tcagcccata
    15901 tcatgtgttg cggcagcacc ggtaccggtg tctgtctggt cagactttcc ggcacgctcc
    15961 cgcaaatgac gccggttttc agcggcgctg gcgtgttgtt gcaggctgtc agcaacatca
    16021 gcagggggag aagtgcgtgc gcactcatcc gcctgtaagt ttttccgcat cgtttcacgt
    16081 cgttcttccc ctctggctgc acgctcctga tcttcgcgcc ggatagcggc cagcgctgac
    16141 tgtgtctccg ccgtggcctt aagcaactcc cggacggtcg tattgctctc tttcacggct
    16201 ttttccagcg cttcataacg ggtgctctcc tgacctgagc gataggcccg gatgaccacc
    16261 attgcctgca gcgaaatggt cagcagcagc gcacaaagga taatgttcag tttcatggct
    16321 gcgctcccgc gtctttcagg caccagttct taaaatcatt ccggcggtta accagtccct
    16381 gcgtgcgcgt tccgccactg ttcacaaaat ctgtcagccg gtaacacatg aggcgccatt
    16441 gccgtgtctg cgcgtgtttc cagatggtgg tgcgttgcgg cgtgccgttt ttgtttttaa
    16501 accacatcag attccggcag cccaggttaa aggcggcgtc cgtcatggct tcgaatgcag
    16561 actgcggcat ctgctgcccc ctgaaattct gtgtgatgca gttctcagcg cggcgcatat
    16621 cgttgatcca ccgcccggcc acttcctcct gtgtgtacag gcggttttcc acgttaccgg
    16681 tggatccgca tccgaccgta ccgaccccca ccagatcacg gtagggtcgg gcccggcact
    16741 cctcccatgt ggcaattttg atttgtgcct cgcgggaggt tctcagttct ccgggagcca
    16801 gggagatccc cagcgccaca atggcggcca cggcgtaacg cttaggtccg gccatcgtgc
    16861 tcatcctgct tcagaagcat cattgcccgg cgctctgcgt cgttcagctc ccttccttct
    16921 gactgcataa ggattttttc aatcagttcg ttgcgccttg cctgggcgat ctccatcctg
    16981 cggcggtgaa gccaggcacg aagcagtgac agcgttccca gcacaaaacc ggccacggca
    17041 attttttcgc tcagcgtcat cacgccgata ccggctgtcg tcactgacgc ccagaacgtg
    17101 atgtgatcac aaagccgctg aaacattctc acccccacaa ctggatcatt tcacgccggg
    17161 tctgcccctg cagctccggc agctccactt cctgtccggc atgcagaaac atgacggctg
    17221 aaatccccgg attggcggcc agtaccgcct cagtcacccc ctgcgtggtg ccgtaatggc
    17281 gctgacagag taaatcgacg gtgtcaccgt ccagagccat tactttcatc agaccagctc
    17341 cgcataaatg cgggtgacac cgcgtatgtc tgatattgcc cagcgggcat cccgccacaa
    17401 atcctcaacc tgcatgtcca gcgcgtcagc cctgcggtcg cctttatcgg tggtatccac
    17461 atcccgggtg cgctccagta caaacgcgcg ggtgagtgcg aaaacggccc tgcggtagcg
    17521 gtggacgtgt acactctctc cgttcacctg cgtggccgga acgtcagcca gtgaggccgc
    17581 gccttcagcc atccgctcac gtttccagag ctccagttcg gcgatcacat gggcgaccgc
    17641 ttccgttgct gcatgtaaca gacgggttgt tgttgtcctc cccggaatgc gggaagccag
    17701 gcgaatttca tgcagtgaga tcgccggcca gaagtcaccc gcgctcacca tggcgtcgcc
    17761 gtcatcggtg tcggtggtat ctgtgtcagc gctgttcacg cggggtctgg ctatcagact
    17821 catgcctgtc tccgtaaaaa tcaggcggtg ggcaaccggt aaaaagaacg cgaagcctgt
    17881 tcagatcacc ggttgcgccg cctgccgacg aggtgcggtt cgttaaatca gtttgttttg
    17941 cgcgcggctt ttttctggct ggcagcgctt ttcttttttg cgctgctctt tttcccgccg
    18001 gtggtggttt tcctgcccgt tgtggatgca ggcaccacct gatttccgtc tgccagtttc
    18061 ctgaccgcgc gctccagcgt ggcaatttca cgcttcactc ccgaagccgg attgatccgc
    18121 atggcttcac gtaacaggct cagcgacaga cgcagatcat cgggagagga ggaggcgcgg
    18181 cgggaaaatg cgcgtgcctt gtgcagtttt gacctgacct ggtccggcat atcctgctca
    18241 ctgaccaggt ggttcagctc atcaagcatg gcggtgtaca cagacaaatc cgcctcgctg
    18301 tccgttgtgg cctgcgtcag aacggcgtca gtaatctctt cagccagtac cgtggcggcg
    18361 tcacggccaa agttatccgg cagcgccaga ccatggcgga tcacgtactc cccgatacgc
    18421 agtgccagcg ggtagtcacg gcagtcaatg gcccacacca tcatggtggt gatgacctca
    18481 tcctggcgtc cgctgtctcc tgccagggtg ccttcaatcc acccttcaaa gtcaggcaac
    18541 agatgttttt taagctctgc cctggcgacg tttgactgaa tacccttcag ctgcatctgt
    18601 gccagcctta actggtgaag gacctgttca tgtgcggttc tttcccgttg tgtttcccgg
    18661 gcgttcatgg tcacccggtt gcgggccacc gcctgttgca catagcgttg tgccggtgtc
    18721 agcatttctc agctcctgtt aacgggcgcg gcgcgcccgt ccgggttatg cttcgggttg
    18781 cggctgtccg ctttcggcgt tgttgtccgg atcttcacct gcgaaggtga tcccctcaat
    18841 cagcgcgcat tttccgtagt cttccaccac gtagcagtca ttcatggact ggtaagtcgc
    18901 gatacggttg tattccggct cctcgcgaat gaaacggcgc agtgagcctt tctggaagta
    18961 gatcgacagg tttttcagcg acgtgatgag catggcgtct ttcgggaagt acggcgcaag
    19021 atacgtctga agaccaccga cggtacggga cgccacaatc agctgcccgg caacaagctc
    19081 agtgttcggg ttggtggtgc tgatggcgtt aatcatggga agacgcagtg tattgaacag
    19141 atcccgcccc atcagtacga ccagatccgg tgcgtcctta tgccattcat ccagcacgga
    19201 tgagcggatg tcgtgcacca tggcatccgg gttaccatag tcaccttttg ccaccagttt
    19261 gttttcatca tcgcgacggg tcagggtcat ccctttaacc acgcgggtgg cagccttgtc
    19321 gcggatgtgt ttcagccagc ccgggttgac gtcctgcagg agagggttag tggcacgatc
    19381 ggaaatcagc gcgtgtgact ccccgttgaa accaatcatg attcgatcca gggctacctg
    19441 gcgggtgatt tgcgcgctca gcagttgctg aaagtttttc tggctgctcc atgcgtcaat
    19501 ctgtgcgtag ctgatgaagg tatcgtagtt cacctgctcg cagcggtaac ggcgtgcatc
    19561 cagatcagca actgatgcag gattgcggcg tttcactccg tcgctcgttg cgttcgtgct
    19621 ggcaatgggg ccgctggtgt caatcaggac tttttcacct tcctggtcat ccacaccgat
    19681 aaaattgatt tcctgtaaca gtccggcgct ttccttgatg gcgttctcca tcttctgctg
    19741 aatggatgga tccacgctga aggttttacc caggcgctct gttgtgatac cagccagcga
    19801 tgcctgctga atcatatagc gttccagact gtcacgtcct tcacgggaaa gggataaacg
    19861 ggccataaat tactcttctc ctgtcagaat tcagccagtg tctggtcagt gccgccagcg
    19921 gaagtaaaac gctgctgtgg atcggcatcc tggtttctca gttgcgcttt cagtgctgca
    19981 agctcgtgac tgagctcatc cagcgccgtt ttgtcgaggg catgtttctg ctccagagtg
    20041 ctgaaacggt cgataagatc tgcatgatcg gatgcgatgt tttccacaac gtcgcgcagc
    20101 tggctgaact gctcgccatc cgctgtgcgt gttttgctga tgagtgccat gacgcggctg
    20161 aaccactgac ggctctcttc cgttcgctgt tctgtggtgg taagcatctc tgcttccatg
    20221 gcgacggtga acatgggggg ttctgttgtg cacttactga actgcatgat gctggcgcgt
    20281 tgctgtgcgg caaactccag gcgttcagtg cccagacttg ctggcgtgtc ggtcattgcc
    20341 agacccacaa tgtaaggccc cttgacggcg tccagctcag gcaacatctc aatgctcgaa
    20401 tacacttttt tcccgtcagc aatcatgttt ttcatgcggt cggtgggttc aatttcggca
    20461 tataacgctg tacgccctgc cagtttccct tcggtgattt catcagttga cagagcgatc
    20521 agatcaccca ctgcgcagag ttcactggat ggccacatcg aaaggacgtg ctcaatgttc
    20581 acccgggagg cgtaaacgga cgggtcatag ttttctgcgg cctgacgcag catttcacca
    20641 gaaatttcac gtccgtcgat ggttgaaccg gaaacggcaa cgcgaaattt tttgcgctct
    20701 ggcgtgtttt tgcttcccat ggaaattctc cataacctgg tcgtttttcc catgatggcg
    20761 gcagataacg gtgtgtctca aggtgcttaa gttgtatggc gacggggaca acgcgggctg
    20821 gtatcaggcg gtattgctga ggcttaatct tgccctgtta atgtcagggg gtgcaatgat
    20881 ccaggatgtc tttattcgta tgcgggcgaa acagctttac tggcaggggt ataccccggc
    20941 ggatatcgct cagctgatgg gaataaacca gaacacgatt tattcgtgga aaaaacgcga
    21001 tgagtgggat gccactcccc cgatccagcg ggtcacccag tccattgatg cacgcctgat
    21061 acagctcacc gccaaaccgg ataaaagcgg cggtgacttt aaggaaattg acctgctgac
    21121 gcgccagctg aaaaaactga atgacggtca gggagaacag gctgtttccg gtaagaagcc
    21181 ccgcaggcgc aaactgaaaa atcacttcac cgaagagcag attgtcgcac tcagggaaaa
    21241 aatcctgccg tcactggcct ggcatcagcg cgaatggtac gacaaccgcc atcaccgcaa
    21301 ccgcatgatc ctgaagtcgc gtcagattgg tgcgacctgg tattttgcgc gcgaagcact
    21361 ccttgatgca ctgcgtgatg atgtctccgc cgggtaccag cgcaaccaga tatttttgtc
    21421 cgcatcccgt cgccaggcgt atcagttcaa acactttatt cagcaggcgg cgctggaagt
    21481 tgatgtcgaa ctgaaagggg gtgacaagat tgttttgtct aacggtgcag agcttcactt
    21541 ccttggcaca tcggcagcaa ccgctcagtc gtataccgga caccttaaat ttgatgaatt
    21601 tttctgggtc agtaactttg tggaactcag gaaggttgct ggggcaatgg cgacactgga
    21661 agggcttacg cgaacctact tttcaacacc gtcaggtgaa acacatgagg cgtatcagtt
    21721 ctggacgggg gaccgctgga acagcgaaca gccacgctca aaacgcagtg agtttgatgt
    21781 ttcacatgcg catctgaagg ggggcgtgct ctttcctgac gaaacatggc ggcagattgt
    21841 cactattgag gatgctgtcg ctaacggatg gcggtacacc aatattggtg ttattcgcgg
    21901 cgaaaataca gaaagtgaat ttcgtaatct gtacatgtgc gaatttgtcc gtgatggtga
    21961 ggcagccttc agcctgagtg cgctcaccgg ctgcggtgtt gacggctatg acgaatggcc
    22021 tgactggaaa ccttttgctg cccgccctat gggggtgagg gaggtctggc ttgggtatga
    22081 cgccaacggc agcagcggga agggggactg cggggcactg tcggtgtgcg tgccgcccct
    22141 tgttgccggt gggcgttttc gcacggtgga aactgtcagg gttcagggga tggagtttga
    22201 acagcaggcc gcactgattg aggaatacac ccaaaaatat aacgtgaaac atatcggcat
    22261 tgatgtgacc ggcgggaacg gggagggggt ttatcagatt gtgaaaaagt ttttcccgat
    22321 ggccgtaccg tacaacttca atatcgcctc caagcgggca ctggtcatga aaatgcttca
    22381 ggtggtgcgt gccggacgct gggagtatga ccgcagtgaa cgtgacctga tccaggcatt
    22441 cgcatcggtg cgaaaaatac agacacccgg cggctatatc acctacgaca ccgaccgctc
    22501 ccgtggcgtc agccatggtg acctcgcctg ggcaaccatg cttgctgtta tcaatgagcc
    22561 aattggtgcg gagcgtgagg ataatcgcgg ctttgcaatg gagttctgaa cacaatgagt
    22621 aaacgcaaaa aatacaccgg aaagcggggg caggattcgc tacacgatgc actgaaatca
    22681 acgcctgcac tcagcgcatt cacctttgat gggccctatg aagtcagcca catggatctc
    22741 ctggataaca tgtcatgtgc agataacggg cgctactttg atacccccat ggactggaac
    22801 gctatcgccc gtgcatcccg tcgtgcgtcc tggcatcagt cggctttgta cttcaagcgc
    22861 aacgccctga acgggtgctt tgttcctcac cctctgctgt cccgccaggc gttttcagcc
    22921 tttgcgctcg actggtttgt gtttggtaat gcctatcttg aagtgcggcg taacaggctg
    22981 ggggaaccac tcctgcttcg cccggcactg gcaaaatata cacgcagggg aagtgacctg
    23041 gatacctact ggtatctcaa cgacgacggt acggagttca gtttcaggaa agggactgtg
    23101 tgccacgtgc ttaatcctga tattaatcag gagatctacg ggatgccgga atatatcggc
    23161 gggttgctgt ctgtcagcct gtcaaactca gcggatacat tccgcaagct ctactacgat
    23221 aacggctcgc atgccgggtg tattatctat gtaggcacac ctcaggcgaa tgcggaaagt
    23281 gtcgaggcca ttaaaaaaac gcttacggat tcccgtggtc gcggggcgtt taaaaacctg
    23341 tttcttcatg cgccgggtgg tggcaaggat ggtgtgcaaa tactgccgtt ccagcagata
    23401 accgcaaagg atgaattcct gaacatcaag gggagcgccc gcgacgatat ccttgctgcc
    23461 catcgtgtac cacctcaact gatgggggca atgcctgacg ggaatgcggc gtttggtgac
    23521 gttgagaaag cggcgagggt gtttttcatc aacgagttgc aacctgtcat ggaggccatg
    23581 aagcatgtca atgaatggct tggcgttgag gtgatgcgct ttaaccccta tgccctgctt
    23641 ctggataacg ccagctgacg cgcttcgctt gtctgctgct tcgccggggc ataaaaaatt
    23701 tatgccccga ctctccagct ctcttaacgg aatgcggatg gcgttcttct ttgcagcttg
    23761 ctgataatat gcatcttcat actgtttact atcggcagcc attccctgtc accgtctccg
    23821 ttgatgacca cgcgaatgga ggtatcatct tccacgcgaa cccgaagaga tacaccgtct
    23881 tttccgacgg aatgtaactc cattgccagg actatgttta ccgactctcc ttcatcgtgt
    23941 gagtcaaccc atgttcttac agccatccgg aatatgttat ctgtgtcggt tgcaatcata
    24001 tttaccggaa atggcctgaa gtcccgatcg tttctgcacg gaaaccccgt atcaacgtaa
    24061 cgatgatgct cgccgtcttt gtcttcccat gtctctgaag gcaattccag ccactgctca
    24121 taaaaagcaa ccagcttttc agcctgattg cgcagaatcc ctgtaacctc ccgcctcgcg
    24181 tcccggctct ttttgtttgc caggtacaaa tcgcgaaaat ggctcatgtc gctctccttt
    24241 ggtctgttta tgccagcctg tattctgcgc tcacctcata ttgtgcgcaa gttttcaccc
    24301 gtgaatgact gatactttaa attctattaa ctttatgatt tcagtgattt ttgtctctct
    24361 tttttatccc gttcgtcacg tccgtttccc tcccgatctc cgacctcgcc agacgcaatc
    24421 agacgcattc tgacggcctg tttccctgac catgttttga tgtggttttt caaaactgac
    24481 tttactgcgt gcgtatcacg cgccatacga ccccaaataa agcaacccgc cttccatccc
    24541 tcaattcggg cgcccttccc tcgtcgcctg cgcgctgcaa ttgcctcgtt tttcgtgcac
    24601 cgtgcaaacc ggcctcagcc agcgccgtta ccgggcggaa tggggaaaaa gtacattaga
    24661 aaaattgtgc aatttcgtgc gcttttgtgc agttttgatc ttgcgatttt ttgtggcagg
    24721 tggtgatgat attcatctgt ctgaatcagt gggagactgc cgggtgagaa ctgtgaaaaa
    24781 ttactcatat ccttatttaa cttttgtcac gctttctgtt ttggggggga tttttctttt
    24841 tatttggtgg cgaaacgatg tttacagaat tacctacctt tattcgttca cgacaaactg
    24901 ccttgtttca gtgtctgtgg tgctttcggt ttttgtctca ctgtgcctgt tgaaaaagaa
    24961 cctggttgat cggtctaaat ttaccggggt tacatcgtat cttaagtttt tttcgggtat
    25021 gtgccttctt acctggatga ttctgtttac cctcctggtt acgctgatat attttcttcc
    25081 gggtgaagat tcattctatt ccgctcccta tgagtacagt cgcggtagct caaaaagttg
    25141 ttcaggggca tttgttgatg atcctgattt gcaaaaaaca atatttattt gctacccata
    25201 cggtgactat caggatggta atgtgattta tgtgaagaag cgtgtgaatg cgctgggggc
    25261 ggtggtcacc tatgcatacg cgacaagcgg ccgatttcgg tttgactgaa aaaagaaatc
    25321 ctgtgttacc ctcgctgctg agggtaacat gcgtgaagtc atcgcccggt tcttgagctg
    25381 ataatgcttt gcgccagcaa ctgccagccc tctttctgtt gcccgtactt gtcctgatac
    25441 ctgtttacct gcaagtttcc gtgcaccaga atcagatcgc cttttctgtg ttttgccagt
    25501 tcttcgtgct gcttcccgaa tgcaataagg taaagccaca ttgtggttgg ctgttctgcg
    25561 ccatggcagg gtagtgatac cgccagttgt gccgttgtga cgttggttcc tttggttgtt
    25621 ctccttgtgt tcgggtctgc tgccagtcgt ccctctgttg caacctgtgt tggcatattt
    25681 gtctcccgtg ttggttttat tgttggggtt agatgactgg cagctcttcg ccagcgccgt
    25741 agataaacag aattcgcttc atgatttcgc tgcgcttgca ggcttcaatt acggacggct
    25801 cttgttgcca ttctgcccgt tgcgccagct ccagtcgttc cttttccgtt agcttccggt
    25861 aatgcgttat cttgtccgtg tacccgtcca caaccagcag cccttcgcgt accatcttcc
    25921 cggcggcctg attaatgatg gtccttttca cgcccagttt ttcaaccagc tctggtgcgc
    25981 tgaagcgtgc gtgcgtttcc aggtagcgta aaatgattac ctttccggtc attctgggtg
    26041 tcttcatttg gcggctcctt cgttgttccg gcgttcgcgg agttcggtca gtgctgccag
    26101 taaattttcc caccatgttt cttccacaac cggatttcgt cgtgaaagca gctgctgcgc
    26161 cgactcaatc attttcgtaa gctctttgtc gctgatcctc ctgatgtcgt tcatggtgtg
    26221 ctccttcggt ttgctgtttc tggtcatgtt catggtcttt tgttacctgc gtgattgcct
    26281 gctggcggcc gaatgcacat agctgacaat tgccgctttt cggttaacga cggccagcgt
    26341 tcttgccagt tcttcgttga gttttctggt ctgtttttcg tggtatgcct gcatgatttt
    26401 tcctacggga gtcagtggtt caggctctga tatggcgcga cgagggcgca cggcaacgat
    26461 gaatgcgctt ctgtcggtgt tgattcttgc agtcaggatg tcggtcataa cggtgtctgt
    26521 gctgcgatcg ggactggcga tcgtgatttc cagttcgtcg cctggctgcg gtcgaacact
    26581 gacgccgctc agtgtcagtg ccggaatgcg tgcgttcatg atcgtgctga aatcatggcg
    26641 ctggagtacg caatggaatc gtgtcatggg ttaacctccg gttgatctgc tgccaccagg
    26701 cttcgtgatt tttcatccca gtgcacaatc cgcccgtcag gcaggcgaac gcgtgcacct
    26761 ctgtgtacgg aaagtgctgc gccactgtgt agctcctcgc ccgtcgtgct cagtgccgac
    26821 ttgatcacgg tggtggtgac gtcagtcagc ggcaatgctg tacgtacatg tatgacgtca
    26881 tcgggcgtgg tggagccgcc ggttttcacg gtttttgtgt cagatggcgc atctttcagc
    26941 tgcctggtga gttgttttct ctctgtgggg gtcagtgacc ttaaccattc gcccaaatca
    27001 ccttccggga tggtcagttt ttgttctttc ctggtttccg gtggtggggt tttaccctct
    27061 ccggtgtctg ttttttcatc atggggacag ttattgccac gagtccaagg tgcggcgaag
    27121 ccgcccccaa cgtcaaaatc ctcgtccgtg ctgttgtcgg ctttcgcttc ttttctgacc
    27181 agtttccact cgtccggatg ggtgcagatg gtgtagcgct cgccgagcga cggggcacaa
    27241 attccgtaga tgcgctgcgg gaattcgccg tagtcgttgg cctcatctgc ggtttcgtat
    27301 gcggtgcgga tcaggtacag gttgcgtgga atgagcacgc cgccctgacg gttgatgtag
    27361 gtggcaaaac accccacgtc agcggcggcc agaatggcgt caatgtcgtc atggtccagg
    27421 cgcttcggtg atgcggggtt acgctccatt tgtccggcaa ggcggcggag ttcacgccat
    27481 acctggcggg gcgggatacc gaagaactgg aactgccgga tgcggtggag actcgcccag
    27541 gcaatggcgt tttccacggt ttccgccatg gtttttcccg attctttatc ggcgtaaggt
    27601 ttgccggttt tcgggtcatt gtcgtgaaag gtgctggcgt ccaggttctt gccaatatag
    27661 gttgcgatgt agcttgccgg tgtgcctttt gccgggtcga ttttttcgca cttgaagcgc
    27721 ggagaaatat cattccccag ttctgcgcga tctgatttga ttgctgcgtc acgcatgatt
    27781 tcaatgatgc gttcttcatt gtccggggcg gtgaatacca gcatgtgcca gtgcggtgtg
    27841 ccgtcatggt gtggctcaac ggtgcgcacc ccgtaccagc tcaggccttc tttgttgagt
    27901 ttttttcttg cagctgcaaa gacgtcgtaa accagataat cactggcatc gcgcacggtg
    27961 gcaccgttgt atttcgggtt caggctgccg tcctcgttta cgctgtgaaa gcgggaaggg
    28021 caggtcactg tcacgaagaa cgcggagtca ccacgcgatt cggcgacctg ttccatccct
    28081 ctgacgcagg ccatcatctc gttgcggcgg ttgcgcggat tccccagccc tgaccagtac
    28141 acgtcctcca gactcaggcg ttcaccgtct tcgttttcaa tgtcccagct tttgagaaaa
    28201 tcacgggtgc gctggcgttt ctcccggaat tcctggagtg actcatggct gatgtagggg
    28261 gatgccttgc gcgatactct tccggcggca cgcagtaatt cctcccgcca gagatcagcg
    28321 aggtggcgca gctggttgtt ccaccaggtg gcgcaggtga tacgcattac cgcacccggt
    28381 accagttcag ggtctggtgc cgtgcggcgg tcacgtctga tctcgagtga cggccagtat
    28441 ggtggggtca cgcccagttt taacgccatg cgcgccagct gctggtaagt cagcaaccag
    28501 gtgaacaggt tatcgtcacc ttttcctgtc cgctcagtaa cttcatcaca tatgcgttcc
    28561 agttcggtgg aaaatgccgc cgccgttagt gtggcaaggc gctgaatttg atcacggttg
    28621 agttgtggca gcatgagtaa ttcatcaagg cgatgacgcg ctgcggtggc tttgaatgcc
    28681 gtcgtggcga agcattcatt cacctgttcg atgcgtttca ggcgtgtgcg cagtccacgg
    28741 cgaacgaagt tatcggccac gtgagtgttg ttgtttttca cgccttcatt gcgggcgctt
    28801 tgttgtttgc ggtttaaacg cgccagatca cgaatgagca cgttgccaag aaacgagggc
    28861 agggcgcgaa gttctgccat ggcttccgat accaccgtgg gcggattctc gcgtttgtgc
    28921 ttcgccagtt tttccagtct gatccgacgc tcttcgttgt ggtgaaggtg cgaattaatt
    28981 cgcttccctt cgtctgtggt tgtcagaaag gcgacggttt cttcatccag tctggcatca
    29041 atgcgaatgt cctcgttgat gtagctctga atggcctggc gtaatgcggt tggctgtgct
    29101 ctgccgaacg tttcaggatc gataaccggt agcggggcgt tccacgggta cgccagttgt
    29161 gcgttatggt cgctcatgcg tcgcactccc cggcagttgc cacctgaaac gccgcgcggt
    29221 acagcacgag aaggcgctgg tattcgccga taaaaccttc tgcggtgtat atacctttca
    29281 cggccacgct gtgggttgtc atttccagaa tcagcgccat ctctgatgac gaccagctga
    29341 tggcggtact gtcggtgccg ttgatgtgaa ataaacccag tccatcacca tcacctttca
    29401 cgtccacgcg gtaacgaccg tccacggtga aggagaaatc cccgtatgtg gtgacgctgc
    29461 gggcagcctg tttacaggcg cggcggtaat agtcgtgcca gttgttcagg cgggaagcca
    29521 gcaggggatc caccgcccac atccagttaa agaagtcctg aatatctgaa agtcccttaa
    29581 cgctcttcat gagaacctcc ggtaacagac gtgcggaagc ctcccgcgtg caggtgtggg
    29641 atagcttccg gatgtggttt atcggtgctg gttgatgagg ctttgcagtt ctgccagatc
    29701 ttcggcgaga tagctgaaga cagcagcgca gtaacggttt gacagcgcgt gcgtgcgctc
    29761 atgcagcatg ttgatatgca tgatttgtgc gacgcgtgtt gcgcggaaaa gtctgcggtt
    29821 gatttcgctg cggatgtgaa gacgctcagc gctggcgcgc tgttgcttgc ggtttgccat
    29881 ggtgtggcct ctttgctagt aagttgtgaa aactcaccat ccagagctgc gaaactgtgg
    29941 gtggcgagac gtacgaggtt cgcagtaccg gctagcaaag aacccggccc gaccgaagtc
    30001 ggccccgtac gccccgccat aattctgacg cgaaaaagac gtggcaatac agtacgcaca
    30061 aaaaaaccgc tggcgcggtt gtgcgctttg ctagtcggca ggctgcgaaa cccggcaccc
    30121 gttttatgag gtgcagcgga aatgtaacc
//
LOCUS       DAZGXZ010000056        29648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000056 DAZGXZ010000000
VERSION     DAZGXZ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.056"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKJF_002576"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKJF_002576"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP0949914.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHKJF_002577"
     CDS             complement(804..1352)
                     /locus_tag="ACHKJF_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP0949915.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKJF_002578"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKJF_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0949916.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGLTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKJF_002579"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKJF_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP0949917.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKJF_002580"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKJF_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP0949918.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKJF_002581"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKJF_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP0949919.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKJF_002582"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKJF_002582"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP0949920.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKJF_002583"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKJF_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP0949921.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHKJF_002584"
     CDS             complement(11782..12675)
                     /locus_tag="ACHKJF_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP0949922.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKJF_002585"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKJF_002585"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0949923.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKJF_002586"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKJF_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP0949924.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKJF_002587"
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKJF_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP0949925.1"
                     /translation="MSLPHLSFADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEVEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKJF_002588"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKJF_002588"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP0949926.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQMIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKJF_002589"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKJF_002589"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP0949927.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHKJF_002590"
     CDS             complement(17843..20107)
                     /locus_tag="ACHKJF_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP0949928.1"
                     /translation="MKITTVGVCIISGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPASGLVMYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTMVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ECWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKJF_002591"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKJF_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP0949929.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKJF_002592"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKJF_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP0949930.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKJF_002593"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKJF_002593"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP0949931.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKJF_002594"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKJF_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP0949932.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYITSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGCF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKJF_002595"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKJF_002595"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP0949933.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFSKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKJF_002596"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKJF_002596"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP0949934.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHKJF_002597"
     CDS             complement(26972..27664)
                     /locus_tag="ACHKJF_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP0949935.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKJF_002598"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKJF_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP0949936.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7197 a         8060 c         7196 g         7195 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgta acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtaagaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcgc gaacggcagg cgcaggttta
     2461 ctacgagatg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtccagca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgca gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgt tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccagccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgtgcg ctgccccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccagcg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttcaga cgtacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcgcggga ggttaactcc tcggtcagtc
     4441 ggctaagttc gatttccatc tgctcgatgg cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgtt
     4561 ccggacgttt tggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcgcgacgg tgtaagcgcc gttcaacgcc gttatctttc accatgcgca
     4741 tcactgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcagat tgtccatctc cgcttcgcag aaagtcagtg
     4861 ctttttccag ctggttgcgg cgggaccggt tattactcag ttgcgcatgc agctcgtcac
     4921 ggcgaattcg cgcccgctct tctgccccgc tatcggcgcg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 tcaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatctccttc gatcagatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttacctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acgccgccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacagcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcaccgca ctgctcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagt tcatcaatat caaaattctt gccctgacgt ttgcagaagt
     6481 ctgccagcag gcgttcggct tcttgctgct cgcgcagacg ctgttccagt tcacttaagc
     6541 gcatccgcaa cggttgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcaata
     6601 gttcgcgagc gacatcccac gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 cagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgcg cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtaac tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atacggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcagtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgtgtcggct gcaccgacat cggcagtccc tgaatggcaa
     7741 acggcttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atggtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ctcttcggtt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc gaaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt tttgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg gctgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagtcc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tgcgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacggc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc cagatcctca ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaacc gctgagcaaa gacgcggttt ttatccatat
     9241 cgatcgcggt acgaataaat ttgtgtacgt ggcggtcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccaactgata atacgatcga gtttgctctg cagatcgaac accagacgat
     9361 cgacgaaatg cagatcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaatttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtt ccggaagttt
     9481 ccgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 cgatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatctg ctgcgcgttt gagctcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatagc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggaggtggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacgggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta atccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtgttattg aactgattga gcgtggtcca tagcacttca acatgttgcc ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagt tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcggggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac ggacttggct gccagttgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggcctt ctgcaaaacg
    13081 atcacgatcc caaggaatgg tggcgcgaga gaagtacagt gcataccctt cagcgtcgag
    13141 aaccactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcaccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgattgtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca ggcgttctgt ccctgactgg tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcgacatcc tcatgatcgg ttgccacgat
    13441 gatgcgatcg gcacctgatt cgcgagcacg ttcaagaaca tgaacaatca tgggtttgcc
    13501 attaatatca accaatggtt taccgggcag acgcgttgac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcacg cagcgggaaa gccaggttgt ccagtttgca aataagttct
    13681 tgtttttctt ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 agacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tgctctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccacc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgctcgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaagga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aaacgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atcatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc accatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgccg cggtgctcaa gcaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt catccagaat cagaatcggg ctatcacgca
    16321 gcaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccatg gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agtccgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gtaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgagtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacat tgcggaattc cacgtcgcca gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagtgat ttcagcggac
    16921 gcatcagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagctcgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcaaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc cttcagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atactacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt ccccgttgac tgtttgtcaa
    17461 aaaatgaaac cggcattccc atcatgtgac caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgaggc tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgtcac ttgaccctga tgtggagtat caagccattg atatccttgc tgttgatagc
    18001 gatgcttaac tttattagag ggcaatcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gtctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt cgtcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtgtgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcggg ccaggccagt cctgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacctg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagcaagc
    18661 ctccagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacattcagc aatgttgatc cacccttccg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcta atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacgggca taagcagaag tgtgattccc agttgcagat
    19021 ggatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actgatacca aaatatcaat gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accatggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattccgc
    19321 caattaatgg tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcgcgc atgatatttt tgatttcttt ggggacggat aaccgttccc
    19501 ccatacctaa accaagaata accgcattcc acggataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcacgc aggctacaat tcggttcaat gacacttgcc tgcagaaaac
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttggcc atgaactgca cgaactttga gtttcattga ccattgttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacatca cgagacctga cgcagggaat atacgtcgac
    19801 cttgtagatg agtaatttga ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa
    19861 caattgcatc ctgcgtcgcg ccagttaagg tttctcctgc ccacaaaatt tgctttgctg
    19921 ataatatgcc ccaaacgaaa aacagcaacg tcagcgcgat ataacggacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagagag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgctaattat gcatacaccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacattca gaagaattta acgaaagggt gaaaactgct
    20221 caaaactctg cgaggcagct tccagttcct gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct cgcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaagcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccaact gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattacttta aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcatcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gccgggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccagatg ggatgccagc
    23041 ggtaccagcg catcttctga cgcaacatca acatggtgat gtaatgccgc caatgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccgccatc cagactagcc agtttttcaa agcagccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt aatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgaccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acggacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggttatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt acgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gaaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgcggt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 cgccacgctg atggctatct tcaaaaagga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggt gtctacgctt gccgcattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgccccgct ttacatatat
    29641 ttgcatta
//
LOCUS       DAZGXZ010000057        29231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000057 DAZGXZ010000000
VERSION     DAZGXZ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.057"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..140)
                     /locus_tag="ACHKJF_002599"
     CDS             complement(<1..140)
                     /locus_tag="ACHKJF_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HFP0949937.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     ED"
     gene            complement(204..1064)
                     /gene="pdxI"
                     /locus_tag="ACHKJF_002600"
     CDS             complement(204..1064)
                     /gene="pdxI"
                     /locus_tag="ACHKJF_002600"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP0949938.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(1296..1886)
                     /gene="paaY"
                     /locus_tag="ACHKJF_002601"
     CDS             complement(1296..1886)
                     /gene="paaY"
                     /locus_tag="ACHKJF_002601"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP0949939.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            complement(1868..2818)
                     /gene="paaX"
                     /locus_tag="ACHKJF_002602"
     CDS             complement(1868..2818)
                     /gene="paaX"
                     /locus_tag="ACHKJF_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFP0949940.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(2919..4232)
                     /gene="paaK"
                     /locus_tag="ACHKJF_002603"
     CDS             complement(2919..4232)
                     /gene="paaK"
                     /locus_tag="ACHKJF_002603"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFP0949941.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(4259..5464)
                     /gene="paaJ"
                     /locus_tag="ACHKJF_002604"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(4259..5464)
                     /gene="paaJ"
                     /locus_tag="ACHKJF_002604"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFP0949942.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRRLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(5464..5886)
                     /gene="paaI"
                     /locus_tag="ACHKJF_002605"
     CDS             complement(5464..5886)
                     /gene="paaI"
                     /locus_tag="ACHKJF_002605"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFP0949943.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(5876..7303)
                     /gene="paaH"
                     /locus_tag="ACHKJF_002606"
     CDS             complement(5876..7303)
                     /gene="paaH"
                     /locus_tag="ACHKJF_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFP0949944.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(7305..8093)
                     /gene="paaG"
                     /locus_tag="ACHKJF_002607"
     CDS             complement(7305..8093)
                     /gene="paaG"
                     /locus_tag="ACHKJF_002607"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFP0949945.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLIPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(8093..8860)
                     /gene="paaF"
                     /locus_tag="ACHKJF_002608"
     CDS             complement(8093..8860)
                     /gene="paaF"
                     /locus_tag="ACHKJF_002608"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFP0949946.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(8857..9927)
                     /gene="paaE"
                     /locus_tag="ACHKJF_002609"
     CDS             complement(8857..9927)
                     /gene="paaE"
                     /locus_tag="ACHKJF_002609"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFP0949947.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(9935..10432)
                     /gene="paaD"
                     /locus_tag="ACHKJF_002610"
     CDS             complement(9935..10432)
                     /gene="paaD"
                     /locus_tag="ACHKJF_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFP0949948.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(10447..11193)
                     /gene="paaC"
                     /locus_tag="ACHKJF_002611"
     CDS             complement(10447..11193)
                     /gene="paaC"
                     /locus_tag="ACHKJF_002611"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFP0949949.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(11202..11489)
                     /gene="paaB"
                     /locus_tag="ACHKJF_002612"
     CDS             complement(11202..11489)
                     /gene="paaB"
                     /locus_tag="ACHKJF_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFP0949950.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(11501..12430)
                     /gene="paaA"
                     /locus_tag="ACHKJF_002613"
     CDS             complement(11501..12430)
                     /gene="paaA"
                     /locus_tag="ACHKJF_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFP0949951.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQHARKVA"
     gene            12715..14760
                     /gene="paaZ"
                     /locus_tag="ACHKJF_002614"
     CDS             12715..14760
                     /gene="paaZ"
                     /locus_tag="ACHKJF_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFP0949952.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            15008..17281
                     /gene="tynA"
                     /locus_tag="ACHKJF_002615"
     CDS             15008..17281
                     /gene="tynA"
                     /locus_tag="ACHKJF_002615"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFP0949953.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(17339..18838)
                     /gene="feaB"
                     /locus_tag="ACHKJF_002616"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(17339..18838)
                     /gene="feaB"
                     /locus_tag="ACHKJF_002616"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFP0949954.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            19074..19979
                     /gene="feaR"
                     /locus_tag="ACHKJF_002617"
     CDS             19074..19979
                     /gene="feaR"
                     /locus_tag="ACHKJF_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFP0949955.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGHLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(20151..20480)
                     /gene="ydbL"
                     /locus_tag="ACHKJF_002618"
     CDS             complement(20151..20480)
                     /gene="ydbL"
                     /locus_tag="ACHKJF_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP0949956.1"
                     /translation="MMKKTLLFCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(20485..20670)
                     /gene="ynbE"
                     /locus_tag="ACHKJF_002619"
     CDS             complement(20485..20670)
                     /gene="ynbE"
                     /locus_tag="ACHKJF_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP0949957.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(20667..23306)
                     /gene="ydbH"
                     /locus_tag="ACHKJF_002620"
     CDS             complement(20667..23306)
                     /gene="ydbH"
                     /locus_tag="ACHKJF_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP0949958.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMEKSQMPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            23514..24503
                     /gene="ldhA"
                     /locus_tag="ACHKJF_002621"
     CDS             23514..24503
                     /gene="ldhA"
                     /locus_tag="ACHKJF_002621"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0949959.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            24614..25036
                     /gene="hslJ"
                     /locus_tag="ACHKJF_002622"
     CDS             24614..25036
                     /gene="hslJ"
                     /locus_tag="ACHKJF_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP0949960.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(25033..25299)
                     /gene="ydbJ"
                     /locus_tag="ACHKJF_002623"
     CDS             complement(25033..25299)
                     /gene="ydbJ"
                     /locus_tag="ACHKJF_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP0949961.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            25573..29097
                     /gene="nifJ"
                     /locus_tag="ACHKJF_002624"
     CDS             25573..29097
                     /gene="nifJ"
                     /locus_tag="ACHKJF_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP0949962.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQVEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         7133 a         7859 c         7201 g         7038 t
ORIGIN      
        1 ttatcttcag cggtagccgc agaaaaaaat ggttcaatat caactgtatt tgcaacacat
       61 cgaagttccg cctgtactgg aagactcagc ataaacaggg gaagtaaacc tatccagccg
      121 caaatcctgg ccttaaacat atgcactcca tgccattaaa aaagaaacaa aagggaaatg
      181 catctttccc ttttgtatat tcgttattct cgcgaaatac catccaacgt agacaacact
      241 tcctcagaaa gatgcaattt ttcagcagcc atattctccc gtaaatgcgc aacagaagac
      301 gtccctggga tcagcaaaat attcggtgaa cgttgtaaca gccacgccag cgccacctgc
      361 atgggtgttg cacccaggct cgcagcaaca tccgacagcg tagaggattg cagcggtgta
      421 aagcccccga gcgggaagaa cggcacgtag gcaatgccgt cacgagccaa agcatcaatc
      481 attgcgtcat cagcacgatg agcaatgttg tattcgttct gcacacagac gatttcggca
      541 atcttgcgcg cctctgcaac ctgcgtcggt gtgacgttgc ttaggccaat atgttttacc
      601 aggccttgtt gttgcatctc tgccagcacg gtcaggctgg cttcaatcga tccttccgct
      661 gggccatgac catcccccat cataatgcgc aagttaacca catccagtac gtccagcccg
      721 agattacgta gattatcgtg caccgctttt tgcagctctg ccggagaaaa tgcgggcaac
      781 caggatgcgt cctctccacg ccgcgcacca attttagtga caattgtcag gtcgtcagag
      841 taaggataaa gcgcttcgcg gataatctga ttggtgacgt gcggaccata aaagtcgctg
      901 gtatcaatgt gattgacgcc caatgccagc gcctcgcgca gcacggttat agcgacgtgg
      961 cgatctcttg ggggaccaaa aaccccagga cctgccagtt gcatcgcgcc ataaccaaga
     1021 cggttaacgg attttgtacc gagagtaaat gtattgctgc tcatcgcacc ctgccctgta
     1081 ataaaacgga ttgagaaaaa tcagtataga aacgaaaaga gataaataat gcgaaggcgt
     1141 aaacaagatt actggatgag ttgctctatt gtcctgaaag acaccaaaac aaaacgcgta
     1201 aagcgccgca ttcggcaata aaaaaaccgc aacctgtatc agcgttgcgg tttaggtatc
     1261 acatgctaaa aattagaacg ttcagtatct ggacgttact gtttcggtcg cagattctca
     1321 tcaaatacca ggcgtttcct gccaggttca acttcccgca atggttcgac ttgatgtaac
     1381 gtctgcttac agcgtgtcac cagcacctga tactcatgcg taccctgctt tttccatgcc
     1441 agctcctgct cactgagttc acgaatcgct ttcgccgggc tgccgacaat caggtaattg
     1501 gcaggcattt ctgctttggc tttcacaaat gcggatgcgc caacaatgct gttctcgcca
     1561 atcaccgcgc cgtccatcac taccgcgttc attcccacta atgcattgcg gcggataatg
     1621 caaccatgaa ggatagcgct atgaccaata tgtccatctt cttccacgac agtatcctgc
     1681 tcgggaaaac cgtgcataac gcaattatcc tgaatgttcg cgccatcttt caccacgata
     1741 cgaccaaaat cgccacgcag gctggcattt ggcccaacgt aaacgccctt gcctagaata
     1801 acatcgccga tcaataccgc tgtcggatgg acaaaactct cttctggcac aaccggagtc
     1861 agaccatcta tctggtaaat tggcatatcg cctcctgttc aatattcaaa ccgccaaaac
     1921 gttgaaaata caggcttccc ggcgcaggca gttcaccgac cgaggtttca cctttttcac
     1981 taacgaacgc taaagcagca ggcgctacgc gctgataaat gttgatacac agctgacgcg
     2041 ccgtatgccc tgcccagtgc gccggaagca actcctctgg caacaatggg tctttaagga
     2101 cgacacggcg ataaaaatgg atcagtaaaa gctgaatatg aaatgcacgc tccggggtta
     2161 actcgtctgc tgcctctttt aacagcggca ccagcgggcg gaatgactga ataaaggttt
     2221 cgtacatggc attttgttca gttaaatgcc agcactcttc cactctggca cgcagtgctg
     2281 cacgagaaag cgccagtggt atttgcgctt caaaacaaat cacgttatcc gccacccccg
     2341 cttcatgcaa aagtgtctgt acatccgcca gtttttgcga cggcgatgcc atcaggctgg
     2401 gtgccagtgc gccaaaacct tgccagatca actgcttttt gacatcagcc agcgttgatt
     2461 tatctaaacc ttccgagagc aacaggagcc atttaccatc ccatgcaggt tgctctgcgc
     2521 gataaatttt actttctgcc cgtcgcgtca ggcgcaagcc tttatcactg aggctataga
     2581 aactgcgtcg cccgatgcgg gaaacatcca gccagccttc tttattaaga cgaaacaaag
     2641 cggtgcgcac gaaacgctca ccaaatccca gcccttccag caaagcagcc agactaccca
     2701 gccagatttc accaccacga tgggaaagcg aatcaccata cagagaggag atcaaagatg
     2761 tgccactgac cggaacagcg tttacagcat gttggataaa agtaacaagt ttactcattc
     2821 gattcagtct gttgttattg ttgtgattta tgaatcatag cacaggtcgt atcgacttct
     2881 ggccctggcg ctttacctca ccagggccag acgacacgtc aggcaccaac aatattgcgc
     2941 agatcaaaca cccgacacgc cttgccttct gaacgcggga tactgccaca gttaacgatc
     3001 atcacatcgg tagagatccc caccatcgac ttaatccgat ggcgcagctg gtggcacacc
     3061 tggcaacgct gctcatgcgt caatgtcagg ctgctttctt tcaactccac tttcacagaa
     3121 agtgaatcaa gatgcccccg gcggttcacc tccagttggt aatggggcga taaatgttcg
     3181 aatttgacaa tctcttcttc cagttgtgac gggaagacat ttacaccgcg aatgatcaac
     3241 atgtcatcgc tgcgtccgct gatgcgatcc atgcggcgca tagtccgcgc cgttcctggt
     3301 aacagtcggg ttaaatcacg cgtgcggtaa cgaatgaccg gcaatgcttc tttggtcagc
     3361 gtggtgaata acagttcgcc atgttcacca tcggcaagcg gtgtgccgtc atgaggatta
     3421 acaatttcag gatagaaatg atcttcccaa atggttgggc cgtcggtagt ttccagacac
     3481 tccatcgcca cccccggccc catcacttct gacaggccat aaatatccag tgcggtgatc
     3541 cccagacgcc gttcaatctc tttacgcatg gcctgtgtcc acggctcagc accaaacact
     3601 ccaacccgca gcgagcaacc gctggcatca ccgcccaact gccgctccag ctcttcaatc
     3661 aggttaaggc aataagatgg cgtaaccatg atcatatctg gttgaaaatc gcggatcagt
     3721 tgcgcttgtt tttccgtctg cccaccagac atcgggatca ccgtagcgcc cagacgttcg
     3781 gcaccatagt gcgcgcccag cccaccagta aacaggccgt aaccataggc aacatgaatt
     3841 ttgtctttgg gcgtgccacc tgccgcacgc aaggaacgcg ctacaatatt ggcccagtta
     3901 tcaatatcgt tttgcgtata gccgacaact gtcggtttac ctgtggttcc cgaagaagca
     3961 tgaatgcgca ccacttgttc catcggcacg gcaaaggtgt cgaagggata gttgtcgcgc
     4021 agatcctgtt tggtggtaca gggaaattta cgcagatctg aaagttccct gaaatcatca
     4081 ggatgtacgc ctgctgcgtc aaatttgcgc cgatacatcg ggacattttc atacgcgtgt
     4141 ttgagcgtcc atttcagacg ttgtgtttgc aacgcctgta actcatcaac agacgcggtt
     4201 tcaatcgggt caagctttgt attggttatc attgtagggt actcgcaggt tgatatgctc
     4261 aaacacgctc cagaatcatg gcgatgccct gaccgacacc gatgcacatg gtgcacaatg
     4321 cgtaacgacc gttacgccga tgcagctcat ggctggcagc cagtgccagg cgggcaccac
     4381 tcattcccaa cggatggcct aaggcgatag cgcctccgtt ggggttaaca tgtggggcat
     4441 catcaggcag ccccaattcg cgtagtacac ccaacgcctg ggccgcgaac gcttcgttca
     4501 gttcaatcac gtccatatcg tgaatactca gccccgcgcg ttccagcacc cggcgagttg
     4561 caggcaccgg accaagcccc atcaggcgcg gttccacccc ggcggttgcc atggctacga
     4621 ttcgcgcccg cggtgtcagt ccttgcgctg ctgccatctg ttcactggca ataatcaacg
     4681 cagcggctcc gtcattcacc ccggaggcat tgcctgcggt aatcacccca ttggcacgaa
     4741 atggtgcttt taacctacgt aactgttcca gcgtcgtttc cgggcgcaga tgctcatcat
     4801 gttgtatttc tgttacaacg cctttcttgt ttttcaacac aaccggaaca atctcctcag
     4861 ccagaatgcc tgaggattgc gcttttgccg tacgttgctg actgcgtagc gcaaaactat
     4921 cttgatcttc tcgcgatatt tttaacagtt cagctacatt ctctgccgtt tccggcatgc
     4981 tgtcagttcc aaattgttga gccatgagcg ggttcacaaa tcgccagcca atagtggtat
     5041 caaacatctc agcctgacga gaaaatgcac tggttgcctt gcccataaca aacggtgccc
     5101 gagacattga ctccacgcca ccagcgatca gcaaatcgcc atcgcccgct ttaatcgccc
     5161 gtgcggcgaa ccccagtgcg tccagcccgg aaccacacaa gcggttaatg gttgtgccgg
     5221 aaacactctg cggcagcccc gccagtaacg tcgccatccg ggctacgtta cggttgtctt
     5281 ctcccgcctg attagcacag ccgaggatca catcatcgat acactccgca tcgagacgcg
     5341 ggtttcgcac cagcagctcc cgcaaaggga tagcagccag atcgtcagcc cgaacacctg
     5401 ataatgcccc accatagcgg ccaattggcg tacgaattcc gtcacagata aaggcttcac
     5461 gcatcaggct tctcctgtaa tggtgccgcc gatgcggtga gatttaccgc gaaacagcgc
     5521 aaccgttttt tgttgttggt taacaatttc gatgtcgtaa acaccggttt gcttgccctg
     5581 atgacgtacc tgcgcagtag cagttaaggt gtctccggca aagcctggac gcaaaaaatc
     5641 aatcgtgcag gcagaagcga cggctgccag cccctggcta ttgcaggcgt aggcaaaggc
     5701 agtatcagcc agtgaaaata gctgcccgcc gtggcaactt tgatgaccgt taagcatttg
     5761 tgcagtgacg gtcatggtca ctacagcaaa gccttcatcc attgagataa tgtcgatgcc
     5821 aagcgctttg gcgcaggcat cgttctcata cattgcatgg gcattttgcc aggccttatg
     5881 actcataacc gctctccaga agcgctcgtt ggcgcagcaa tgaacatggg cgatagcgtt
     5941 cttcgccata gtgatgttgt agattttcca ggagccttaa tattcgctgc cagccaagtt
     6001 gcgctcccca ggcaagcggg ccatatggat aattcacccc aagacgcatg gcggtatcga
     6061 tatcctgttc agaggccacg cctttttgca gcgcatcaag ggcttcattg atgatcattg
     6121 ctaccgttcg ccaaatcagc attcctgggt aatctgcaat ttgcagcact gttttgccct
     6181 gctgttgcag gtaataaatg gccttgcggg tcgctgagtc cgggttcacc gctgccgcag
     6241 caatggtcac caccttgccc gccattttgt cgaccactac caccgggcgt gccagtcgta
     6301 ttgccagcgc ctgtgccgtc tcgccttgtg tctcaatcaa taaaacatcg tcaatttccg
     6361 tgacgccgtc acttttcttt tctactttca ttgggctaaa actgtcgctt accgcttcca
     6421 ggccaacaac tgcctcacgt tccgcgcgcc agtcgtacac gcccagccca cttttcttgc
     6481 ccaaccgtcc accaatcacc agttcctgtt gcaccagcga aggtaaaaaa cgacgctcct
     6541 gccagaaagc gttaaacacc gaacaggtga cagcaaaatt gacgtcctga ccaatcagat
     6601 cggttaattc cagcggcccc atcgggaaac cagcgccatc gcgaagtgca gcgtcaatca
     6661 cttctggtgc agcaacctgc tcttccagtg cccgccaggc ctcggaataa taaggacgcg
     6721 ccacacggtt aacgataaat ccaggggtcg aatgacagcg cacaggctgc ttaccccaac
     6781 tcaacgttag ttcacacaac tgctcaacaa cttccgctgc cgttgccagc ccactgacca
     6841 cctccaccaa cttcatcacc ggtgccgggt taaaaaaatg cagccccgca acacgttcag
     6901 gattttttac ctccgcagca atcgcggtta tagagattga cgaagtgtta gtggtcaata
     6961 gcgtttgtgg cgggcaaact tccgccagct gtgcaaagag cgcttttttg acttccagac
     7021 gttcagacgc cgcttcaatg accaggtccg cagctgccag cgcgtgaata tcggtcaccg
     7081 ggatcaggcg tttcaatgtg cgttcacagg tttcagcagt cagttttccc cgcgtcacgc
     7141 gtgaatttag ccgcgcgtgt atcccgtcga ttgcgcgggt cagcgcttca gcagaaatgt
     7201 catacagtaa aacctgatgt ccatgactgg cggcaacttc agcaatgcca gcccccatgg
     7261 tgccgctccc aatcactgcc acagtttgca catttatcat catgctattt ccccgtgaac
     7321 tgcggtgagc gtttagccag gaacgcactg acaccttcac gataatctgc gctccgtccg
     7381 gcaagtcgct gatagtcgcg ttccagatcc agttgcgtat cgagcgtatt ggtttcagcg
     7441 ctatttatcg cttgcttgat aagtccaaga ccaaatgtcg gttgtgtcgc cagatgccgt
     7501 gccagctgtt gcgcggtatc tgccagcgtt tcatcatcaa caacctgcca gatcatcccc
     7561 cattcgtgcg cctgttcagc actcagttga ttccccagca gtgccagccc catggcgcgc
     7621 gctcgcccag caacgcgtgg cagtaaccag gttccaccgc aatcgggtat taagcctaac
     7681 ttactaaacg ccatgacgaa ttttgctgaa cgggcagcaa taacgatgtc gcaccccagt
     7741 gccagtgttg cgcctgcccc tgccgccacg ccattgactg cacagatcac cggttttggc
     7801 agttttgcca ggcgacgtac cagtgggtta tagaaacgtt caactgacat ccctaaatcc
     7861 ggtgcggggc ctgtgggatc aacgttacga tcgttaagat cctgaccagc acaaaatccg
     7921 cgcccggcac ctgtaagtaa caggcaacgg atagtgtcgt cgcgctcgac ctgtttcagg
     7981 cactctgcca gttgtgcgtg catctcatca ttaaaactgt tcaggcgttc cgggcggttg
     8041 agcgttagtg tcatcacgcc cttttctaca tgactgagga tgaattccat cattagcgtc
     8101 ctttaaagtc gggcgtgcgt ttttgtaaga aagcggagat gccttcatga cgatcttctg
     8161 ttgccgccag caaggtgaat aactgtcgct cctgggcaag tccggcttgc aaagccactt
     8221 cctgcgactg gcgcagcgct tgctttgccg cttgtaaggc cagcggcgag tgacgtgcca
     8281 ttttcgatgc cagctgtaag gcgtattcga gggttaaatc gctggggaag acgtcgctaa
     8341 ccagcccggc ctgctgtgct tgctgagcgg tgatactttc tccgctcagc accattttgc
     8401 tggctaacga tttaccgaca ctacggatta aacgttgcgt tcctcctgcg cctggcatga
     8461 tgccgagagt gatttccggc aaaccaaaac gcgcgttctc tccggcaacc accacatcgc
     8521 acaacaatgc cagttcgcaa cccgcaccaa gcgcgtaacc gttgacggct gcgatgagag
     8581 gtttgttgaa ggcctgcaat cgcgcccata gctgcgggcg tgtatcgttt aaggtggccg
     8641 cgagatcttt ttctgccatt tcgttgagat cggccccagc ggcaaaaaag cgtgcattac
     8701 cggtaatcac acagaccgaa atgctgctat cggtagccgc agcttccagt tcatttacca
     8761 gttgcgtcag aagggcatta tttagcgcat tacgtgcggc gggacggtta agggtcagca
     8821 acaatacttg ttgctgacgg ctgacgatca gttcgctcat gccatcccct tcgcgtcaaa
     8881 gtcaaccacc acatcgctgg tcagcggcag tgcctggcaa ctcaacacat agcctgcggc
     8941 cagttcatcc ggttccagac tgtaattggt ttccatcgcc actttgccac gcagcacttt
     9001 gcatttgcag gtcgcacaga cgccgccttt gcaggcatag ggcagatcag ccccctggcg
     9061 caatgccgca tcgagaatgc tttcatcgtc ggcattaagc acgatttccc gatcccgccc
     9121 atcctgacgt acagtcactt tttgtccgtc actttgcacg ttaacgctac gtttgacgcg
     9181 cgtgccaggc gtattaaacc gctccagatg aatggtttta tctggcattc ccagtgcttt
     9241 taaggcggct tccgtctcat ccatcatcgc cgccggacca caaataaatg cctcatcata
     9301 aagacgaaaa ttaatgagcg aggccccaag tgactgtaat ttttcaccgt caatacgccc
     9361 gtgaagcaga tcgctgtcga gggtttcctg actgaaaatg cacaacaact gtaaacgctg
     9421 aggatatttg tctttcaggt ctgccagtgc ctggcgaaac atcatgctct ggctggtacg
     9481 gttaccgtag atcagggtga actgactttc aggctcggtt tgtaaagtgg tggcgataat
     9541 cgccagcatt ggcgtaatac ctgatcctgc tgcaatagcc agatagcggc cctggcgttc
     9601 ggcctgtggc tgatagccga aatgcccctg cggcaccatg acctccagcg tcataccctg
     9661 gcggatgtgt tcgcgggcat agcgggagaa acgtccgcct tcaatggctt tcaccgccac
     9721 actaatttcg ccaggcaggt agctgcggca aatggagtaa caacggcgta attcttcacc
     9781 atcaagactg gcttttaagg tcaaatgttg accggggcga aagcgatacg cctcctgcaa
     9841 aggctggggc accgcaaagg taatggtcac cgcatcacgg gtttccggct ccacttttgc
     9901 caccgttaag gaatgaaacg ttgtcatggc atcctcaaat acatttgaaa taatcgaaag
     9961 gttcgcggca actatcgcag cgatacaatg ctttgcaggc cgtggaacca aattcactga
    10021 taagtgtggt atggacgctg gcgcagcgcg ggcaacgtac ttctggcggc aaatgggcat
    10081 ggcaactgtg tccggcgggc gggctaatgc catactgtcg cagacgctca cgggcatcgg
    10141 gggtcatcca gtcggtggtc catgccgggt cgagttgcag cacaacctga acgggggtaa
    10201 acccgtgggt tgtcattgcc tcacgtatcg cgccaatcaa atgttccgtt gccggacaac
    10261 cggaataagt cggcgtaaag ccgatcaccc atccttctcc catctgtgtc acattacgca
    10321 ccatgcctaa atcggtaatg gtcagcaccg ggatctccgg gtccgggatc tggctgagta
    10381 gcgcccatat ctcatgaacc tgtggcggtg caatggtggc cagacgttgc atacccatct
    10441 cctctgttac cattgctgac cgggcaagac acgctggaga tactgcattt ctgccagcat
    10501 gggtccaagg tgttcggtat gcagtccttt tttaccgcca gtgcgatacg cctgctcttg
    10561 cggtacgttc aatgtggctt cgttgatccc agcaaaaact tcggcttccc acgctgcgcg
    10621 taaagtgcgt ggatcaaccg caataccctc ttcacttagt gcaatatcaa tctcgtcggc
    10681 atcgaacagt tcggcggtaa aacgccacaa cttgttgatg gcctgctgca tcttttgccc
    10741 tgatacgtca gtaccattgc ccagtcgctc cagccagcca cgactaaaac gcaggtgata
    10801 acgcgcttct ttaattgctt tggcagaaat cgccgccagt tgcggatcac ggctctccat
    10861 cagacgggta aagagcgcca catgccatgc atcgatgaaa tactggcgtg caatggtgtc
    10921 ggcaaaattg ccgtttggct gttcaaccaa caataagttg ctgaactggc gctcgtctcg
    10981 ggtgaaggcc agggtatctt catcgccttc tcccgctaat tcagcggcat acgataagaa
    11041 gttgcgtgcc tgacctaata aatcgaggcc aatgtttgcc agtgcgagat cgatttccag
    11101 ttccggtgcg tgaccgcacc attcccccaa ccgctgggag agcaccaggc agttatcgcc
    11161 caggcgcaag gtgtaagccg ttaactgatt catttccgac ctcacatgtg ctcaatgcca
    11221 tcagggatgg tgtaaaacgt tggatggcga tagaccttgc tttcagccgg atcaaaaaat
    11281 tcaccgcgtt cttccggttg cgaggcaaca atttcactcg ccttcaccac ccaaattgaa
    11341 catccttcgc tacgacgggt gtaagcatca cgggcatttt ccagtgccat ccgctcatcg
    11401 gcagcatgta aactgccaac atggcggtgt gataagccct gtttgccacg cacgaaaact
    11461 tcgtataacg gccagtaaac attactcatt ttgaatctcc ttatgcgacc ttacgggcat
    11521 gttgtttttg tgcatgggcc agcgctgctt cccgtaccca ggtaccttct tcccaggctt
    11581 tacgtttggc gtcgagcctc tcctgattac aaattccgcg accgttaatt acttcgttaa
    11641 actcctgcca gtcgatctca ccaaagcggt agtgaccgct ttcagtgtca aaatgcagat
    11701 ccgggtcagg aacggtcata ccgagcattt caacctgtgg aacggtgttg tccacgaaac
    11761 gctggcggag ttcgtcgttg gtgaaacgtt tgattttcca ggcgagactt ctggcgctgt
    11821 ttggcgagtt atcatcgttt ggcccgaaca tcattaaggc tggccaccag aaacggttaa
    11881 tggcgtcttg caacatctgc ttttgagctt cgctgccctg cgccagtgcc atgcaggctt
    11941 caaacccctg acgctggtga aaactctctt ctttgcagat tttcaccatt gctctggcat
    12001 acgggccata agaagtgcgg cataacgcca cctgattgac gatcgctgcg ccatccacca
    12061 gccagccgat aacgccgata tcagcccaac tcaatgtcgg ataattaaag atggaggaat
    12121 atttcatccg tccgtcgagc attttttggt agatgtcttc tctggcgcag cccagtgttt
    12181 cggcggcgct atagagatac aggccatgac ctgcttcatc ctgcactttg gctaacagaa
    12241 tggctttacg ccgcaaggtt ggtgcgcgag tgatccagtt accttcaggc aacataccaa
    12301 caatctcgga atgcgcatgc tgcccaatct ggcggatcaa tgtctttcgg taagcatcgg
    12361 gcatccagtc ctgtggctcg atagccgttt cctgggctat ccgttgctca aagcgttctt
    12421 cttgggtcac gtcatcacct ttacgattcc agttttcgtg aattaattaa aaacacgaat
    12481 cacattgttt tatgaaagtt acacaatggt taactaaaat cacaaatttt gtatgcttga
    12541 actgtgacag atttcgcact ctattttgat aactcattat ttatcagtgc attaatgttt
    12601 ttagtagttg atatacagat cacgcagtta acatttcgtt aaaagatccc ttgcttttta
    12661 tgattcgcga tttaactatt agcaacagaa atgtgaaaca tctggagagt agcgatgcag
    12721 cagttagcca gtttcttatc cggtacctgg cagtctggcc ggggccgtag ccgtttgatt
    12781 caccacgcta ttagcggcga ggcgttatgg gaagtgacca gtgaaggtct tgatatggcg
    12841 gctgcccgcc agtttgccat tgaaaaaggt gcccccgccc tccgcgcgat gacctttatc
    12901 gaacgtgcgg cgatgcttaa agcggtcgct aaacatctgc tgagtgaaaa agagcgtttc
    12961 tatgctcttt ctgcgcaaac aggcgcaacg cgggcagaca gttgggttga tattgaaggt
    13021 ggcattggga cgttatttac ttacgccagc ctcggtagcc gggagctgcc tgacgatacg
    13081 ctgtggccgg aagatgaatt gatcccctta tcgaaagaag gtggatttgc cgcgcgccat
    13141 gtactgacct cgaagtcagg cgtggcagtg catattaacg cctttaactt cccctgctgg
    13201 ggaatgctgg aaaagctggc accaacgtgg ctgggcggaa tgccagccat catcaaacca
    13261 gctaccgcga cggcccaact gactcaggcg atggtgaaat caattgtcga tagtggtctt
    13321 gttcccgaag gcgcaattag tctgatctgc ggtagtgctg gcgacttgtt ggatcatctg
    13381 gacagccagg atgtggtgac tttcacgggg tcagcggcga ccggacagat gctgcgagtt
    13441 cagccaaata tcgtcgccaa atctatcccc ttcactatgg aagctgattc cctgaactgc
    13501 tgcgtactgg gcgaagatgt caccccggat caaccggagt ttgcgctgtt tattcgtgaa
    13561 gttgtgcgtg agatgaccac aaaagccggg caaaaatgta cggcaatccg gcggattatt
    13621 gtgccgcagg cattggttaa tgctgtcagt gatgctctgg ttgcgcgatt acagaaagtc
    13681 gtggtcggtg atcctgctca ggaaggcgtg aaaatgggcg cactggtaaa tgctgagcag
    13741 cgtgccgatg tgcaggaaaa agtgaacata ttgctggctg caggatgcga gattcgcctc
    13801 ggtggtcagg cggatttatc tgctgcgggt gccttcttcc cgccaacctt attgtactgt
    13861 ccgcagccgg atgaaacacc ggcggtacat gcaacagaag cctttggccc tgtcgcaacg
    13921 ctgatgccag cacaaaacca gcgacatgct ctgcaactgg cttgtgcagg cggcggtagc
    13981 cttgcgggaa cgctggtgac ggctgatccg caaattgcgc gtcagtttat taccgacgcg
    14041 gcacgtacgc atgggcgaat tcagatcctc aatgaagagt cggcaaaaga atccaccggg
    14101 catggctccc cactgccaca actggtacat ggtgggcctg gtcgcgcagg aggcggtgaa
    14161 gaattaggtg gtttacgagc ggtgaaacat tacatgcagc gaaccgctgt tcagggtagt
    14221 ccgacgatgc ttgcagctat cagtaaacag tgggtgcgtg gtgcgaaagt cgaagaagat
    14281 cgtattcatc cgttccgcaa atattttgag gagctacaac caggcgacag cctgctgact
    14341 ccccgccgca caatgacaga ggccgatatt gttaactttg cttgcctcag cggcgatcat
    14401 ttctatgcgc atatggataa gattgctgct gccgaatcta ttttcggtga gcgtgtggtg
    14461 catgggtatt ttgtgctttc tgcggctgcg ggtctgtttg tcgatgccgg tgtcggtccg
    14521 gtcattgcta attatgggct ggaaaacttg cgctttatcg aacccgtaaa gccaggcgat
    14581 accatccagg tgcgtctcac ctgtaagcgc aagacgctga aaaaacagcg tagcgcagaa
    14641 gaaaaaccaa caggtgtggt ggaatgggcg gtagaggtat tcaatcagca tcaaaccccg
    14701 gtggcgctgt attcaattct gacgctggtg gccaggcagc acggtgattt tgtcgattaa
    14761 tcggtgaatg aagggcaacg gccaatagtt gcccttttat ttcactaagt tttgtgacgt
    14821 tgtcacatta tgcatgatgt gtacatctat tttcagggca cccactgtat gaaaagctgg
    14881 cacacctgcc aaacaacctg gcaggtgcag gcaatcccct ttgcatcagt actgataatg
    14941 tgaacctgac taaaccgccc acagagcgcg gttgctaaca agaacacaac atctgacgag
    15001 gttaataatg ggaagcccct ctctgtattc tgcccgtaaa acaaccctgg cgttggcagt
    15061 cgccttaagt ttcgcctggc aagcgccggt atttgcccac ggtggtgaag cgcatatggt
    15121 gccaatggat gaaacgctta aagaatttgg tgccgatgtg cagtgggacg actacgccca
    15181 gatctttacc ctgattaaag atggcgcgta cgtgaaagtg aagcctggtg cgcaaacagc
    15241 aattgttaat ggtcaacctc tggcactgca agtgccggta gtgatgaaag acaataaggc
    15301 ctgggtttct gacaccttta ttaacgatgt tttccagtcc ggtctggatc aaacctttca
    15361 ggtagaaaag cgccctcacc cacttaatgc gctaactgcg gacgaaatta aacaggccgt
    15421 tgaaattgtt aaagcttccg cggacttcaa acccaatacc cgttttactg agatctccct
    15481 gctaccgcca gataaagaag ctgtctgggc gtttgcgctg gaaaacaaac cggttgacca
    15541 gccacgcaaa gccgacgtca ttatgctcga cggcaaacat atcatcgaag cggtggtgga
    15601 tctgcaaaac aacaaactgc tctcctggca acccattaaa gacgcccacg gtatggtgtt
    15661 gctggatgat ttcgccagtg tgcagaacat tattaacaac agtgaagaat ttgccgctgc
    15721 cgtgaagaaa cgcggtatta ctgatgcgaa aaaagtgatt accacgccgc tgaccgtagg
    15781 ttatttcgat ggtaaagatg gcctgaaaca agatgcccgg ttgctcaaag tcatcagcta
    15841 tcttgatgtc ggtgatggca actactgggc acatcccatc gaaaacctgg tggcggtcgt
    15901 tgatttagaa cagaaaaaaa tcgttaagat tgaagaaggt ccggtagttc cggtgccaat
    15961 gaccgcacgc ccatttgatg gccgtgaccg cgttgctccg gcagttaagc ctatgcaaat
    16021 cattgagcct gaaggtaaaa attacaccat tactggcgat atgattcact ggcggaactg
    16081 ggattttcac ctcagcatga actctcgcgt cgggccgatg atctccaccg tgacttataa
    16141 cgacaatggc accaaacgca aagtcatgta cgaaggttct ctcggcggca tgattgtgcc
    16201 ttacggtgat cctgatattg gctggtactt taaagcgtat ctggactctg gtgactacgg
    16261 tatgggcacg ctaacctcac caattgctcg tggtaaagat gccccgtcta acgcagtgct
    16321 ccttaatgaa accatcgccg actacactgg cgtgccgatg gagatccctc gcgctatcgc
    16381 ggtatttgaa cgttatgccg ggccggagta taagcatcag gaaatgggcc agcccaacgt
    16441 cagtaccgaa cgccgggagt tagtggtgcg ctggatcagt acagtgggta actatgacta
    16501 catttttgac tggatcttcc atgaaaacgg cactattggc attgatgccg gtgctacggg
    16561 catcgaagcg gtgaaaggtg ttaaagcgaa aaccatgcac gatgagacgg cgaaagatga
    16621 cacgcgctac ggcacgctta tcgatcacaa tatcgtgggt actacacacc aacatattta
    16681 taatttccgc ctcgatctgg atgtagatgg cgagaataac agcctggtgg cgatggaccc
    16741 agtggtaaaa ccgaatactg ccggtggccc acgcaccagt accatgcaag ttaatcagta
    16801 caacatcggc aatgaacagg atgccgcgca gaagtttgat ccgggcacga ttcgtctgtt
    16861 gagtaacccg aacaaagaga accgcatggg caatccagtt tcctatcaaa ttattcctta
    16921 tgcaggtggt actcacccgg tagcaaaagg tgcccagttc gcgccggacg agtggatcta
    16981 tcatcgttta agctttatgg acaagcagct ttgggtaacg cgttatcatc ctggcgagcg
    17041 tttcccggaa ggcaaatatc cgaaccgttc tactcatgac accggtcttg gacaatacag
    17101 taaagataac gagtcgctgg acaacaccga cgccgttgtc tggatgacca ccggcaccac
    17161 acatgtggcc cgcgccgaag agtggccgat tatgccgacc gaatgggtac atactctgct
    17221 taaaccatgg aacttctttg acgaaacgcc aacgctaggg gcgctgaaga aagataagtg
    17281 attgtttcag acaaaaaaac ggcaccaggt gccgtttttt acttatgagc gaaccagatt
    17341 aataccgtac acacaccgac ttagtttcac accaaccgtc cagccagtcg gggccaaaat
    17401 cacggcccgt tcctgactgc ttcatcccac caaacggtaa gttagcgtca attaaggtat
    17461 ggctgtttac ccacaccgtc cctgcctgta agcgatcgct atattccaga gcctgggaga
    17521 gattttgcgt ccagacactg gcagttaagc catattccgt gtcgtttgcc agttgtaacg
    17581 cctcttctcc atccgctact cgcaccaggt ttaccaccgg accaaacacc tcttcacgag
    17641 ttaagcgcaa tttagcatcg ggatttacca ccagcgttgg cgcaacataa tacccctctc
    17701 cggctggtcc attcgacccg cgaatcagct ctgcttgctg tgcctgcgca tcgtcgagga
    17761 atgaacacac tttgtcgcag tgcgcacgag aaaccaaagg gttaatctgt gcaacaggtg
    17821 acatccccgg tcccacttgc aacgatttta ccgcctgctc aaatccacta accagcgtgt
    17881 caaacaacgg cgcttcaata taaattcgcg aactggcggc gcatacttgc ccttgattca
    17941 ggaagcttcc ggtcatcaag ccttcaataa cccattgcgg atcagcatct tttaatacaa
    18001 ttgccgggtt tttaccgccc agttccagcg ttacacgcgt taagtgatca gcagcagttc
    18061 tggcaatacc ttttcccgtc gcggttgaac cggtaaaact gattttcgca acatgaggat
    18121 gtgacgtcag ggccgcgccg catacagcac ctgacccggt gacgacatta aaaacgccat
    18181 cagggatacc agcctcgctg gccagttccg ccacgcgcaa catcgtcagt ggcgtggttt
    18241 ccgaaggctt aatcacgatt gaacagcctg ctgccagtgc tggcatcacc ttccacatac
    18301 caatcatcaa cggaaagttc catggcacaa ttcccgccac tacgccaacc ggctctttac
    18361 gcgtccaggc ctgataacgc gccccctggg gtaagggaat cgacaagtcc agcgttttac
    18421 ccgcgatttt ggtcgttaac ccggcggtat aacgcatcca gttcagcgta cagcccactt
    18481 caaaagcacg ggaaatggca attgacttgc cttgctccag ggtttccagt tgcgccagct
    18541 cctcactgtg ctgttccacc agatcggcaa aacgtagcag aatacgttca cgctctgcgg
    18601 gtaatcgccc ggcccagcga cgcgagacaa aggcccgcca ggcagacatg actgcgttat
    18661 ctacatccgc ttcgttggca tcagcagtag acgcaatttc ttgcccggtg gccggatcaa
    18721 agatcgccaa tcgtttttca ctttgtgcgg ggcctggacg accatcaata taaagaccgt
    18781 gttgacgatc gagaaactgt tggacctggc ttaatactgc tacatgcggc tctgtcatca
    18841 cttttcctta ttatttaccc agtgtgattt cagtgtacgc agcgacaaga aaagtcactt
    18901 ttttcgctgt gacattcgct ttgtcagctg tgacaagctc cgcaaatcgt gacaataacc
    18961 gcttatgtct ctatgcctga gaagacttat ttcctatagt caggctcatt agcttcgttt
    19021 actgcaacac aaatgcaaca ataaaaatac atttcacaga gcgaaaacgt gccatgaacc
    19081 ccgcagtgga taatgagttt cagcaatggc tttcccaaat caatcaggta tgcggaaatt
    19141 ttaccggaca cctgctgact gagcgttaca cgggtgtact ggatacgcat tttgccaaag
    19201 gactaaagct gagtaccgtg acaaccagcg gggtgaattt atcccgcacc tggcaggaag
    19261 taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt caggcaataa
    19321 tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta ctcgatgcct
    19381 cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta cttttgccac
    19441 gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa agactggacg
    19501 ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg aataatccgg
    19561 cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg ctgcgcccgg
    19621 tacttcatca gcgggaatct gttcaacctc gtcgtgaacg tcagtttcaa aaagtggtta
    19681 cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata gccggagaga
    19741 caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg gtagtcgcgc
    19801 aatatattcg taaccgtcgt ctcgattttt gtgcagatgc gattcgccat gccgcagatg
    19861 atgaaaaact ggcaggcatc ggctttcatt ggggattttc tgaccagagt catttttcaa
    19921 cggtatttaa gcaacgcttt gggatgacgc caggcgagta tcgacgtaaa ttccgctaac
    19981 taattggcgt tgtgtgtact gcaacgccaa ttagttaaaa cagatatttg actatgcatt
    20041 tttcagggcg aaaggcaatt tgacaggagt ttttgaatat tcaccttgtt tcaggaaaat
    20101 agatcaatcg ctttctcctg tagcgttatc ccgatattgc tgttttgcta ttaaaatttt
    20161 cgcacccatt taccgtttat cccttgtaca tattcccccg gtttggcccg agctacgagc
    20221 ttttgcccgg cgagtttcgc gatatcatct acagacacat tattttgctt tgccagttgt
    20281 tggtaactcg ctttacgttc ggcattaatg tcggttacta atttctctgt ttcagcatcc
    20341 gttttcagcg caaccagata accataaaat gtttcaccta cccgcccctg agttctggct
    20401 tcatccagag tcaatgccat tacattgctg cttaccagcc caacaagaaa cgcacaaaaa
    20461 agtaatgttt tcttcatcat cgcctcagaa aagatcgcta cgagtttcaa gcagctcttc
    20521 gacatctttg tctgccttga tgatgatctc atgctcaatt ttaacgttca tattgatagt
    20581 gatcggttcc ttgggcgcag cgacttcaat acgcggcgta caggcagcca gcataaaaga
    20641 tgacgtcaac gcagccagta aaattttcat tgtttttcct cacactcttt tccttgcgga
    20701 cagtcatttc ccggcagacg tgcgttctgc tccaaccatg cctggagatt atcgccaaag
    20761 cgtaaactgc gccacagcgt aaaaatattc tcttcatgat agtaattaag gtttaccgta
    20821 ccgcttttac catcaacgcg actggtacct gtaatgttgg cctgcatggt taataaaccg
    20881 agattatcta agttaatttt tgtcgatgac cggctaattt ccatatagcg caaccagtta
    20941 attgctgaac ccgcagtcat attgtctttc accaccgcat ctgcggtgtc tttatccagc
    21001 cgcaaggtca ttggcccgct atttgccagc caaccatctt tcactatcca tttttcgttg
    21061 tttagccata acggcaatgc accactaaat gccccggaca tggcgaattg tttcggattg
    21121 acggcgctaa ccagttcgct ggatgagaga ttattcagcc gcaacagcgc cgggtcatgt
    21181 tgcggcatac gtaattgttt catcagtacg ttaccgccta acacatcgac gctaacatca
    21241 gtcaacagca agggttcttc ttcagtccac ggataacgcc cttgcaaatc agccgtaata
    21301 tttttcgctg tcaccagatt aatcacttcg gcaattcgca acgtaacggg gccgcgagtc
    21361 cccagatgcc aggctccatc ggcaaaacgg aaaggcagga caaaatcgac accgttaacc
    21421 tgattatctg gcatccaggc actaccgcct ttcaacacgc cgtgtcctcc cgcgcggaat
    21481 ccttgttcag gcgcagcaga aaatgcaacc tgagcataca gttcaccatc gcgtaagttc
    21541 atcttccagt cgggtggcac cagcggctgg aatacagtca gtgattgttt tggccaccag
    21601 gcgttaccgc gcagacgaat accgtcccag cgaccattta cccgaaccgg gccaatctca
    21661 ccagcatgta aatcgccttt aaagaggaaa tacgtcggat cgcgcccatc gacgctaaat
    21721 tttaaggttg atggcggtaa cacactgccg ccagtgaaca gcgtttgccc ggcgtccagt
    21781 gacagcgcgc cgctgaagga gggatgctgt gcgtcacgta cccagacgac gggcttgtcg
    21841 agaattaatc gcggcttttc taccgtcatc gtaccgtatt gtaactggtc gaaaccggtg
    21901 gagagatcgg tcagcgtaat cgtgctgtca tgccactcac cttttcctgc gacatcccag
    21961 gtggcattca tcggtgtaaa actgcctttt ccccagtagc gccactgcca gcggccagcg
    22021 tcagggagaa aatcattcgc cagcccatcc atatgcagca cgaaatcgcc cagttcattt
    22081 tcatgcgcct gcaaaatggc ctgcaaacgt ccgtcaacac cacgttgggt gacttttaca
    22141 cccgctaaag gccagcggat ttcatcgatg tccagcgaat cgatgactct tcccttcgaa
    22201 cgtaataacg cgccgggctc aaaggccagc gttgggtcag tcagacttcc acttagctgc
    22261 gcgggtaagc gggcatataa aatgaggtcc gcctgtttcg cttcaccggt cagttgcagt
    22321 ggcatctgac ttttttccat gcttaatttt cctggaccaa aattaagcac cgcgttgcct
    22381 ttacccgctt gcccctgggt cagcacactc agtcgcccgc tgaccagagc gttctcgagc
    22441 cctgcctgcc agttgtcgac tttgacaccc agtcggccac tcaaaggaaa acctgcatac
    22501 ggccagctcc agcgaccatc gcttacggtc aattgttgac gagtaatttg ccacggcaaa
    22561 tcgagcaacg gatcgccgtt atcccgtgcc agcacaatca attgcccgct attttcctgc
    22621 cagtccagct ctgcatccac cagtgacggt tcctgcggca agtttaacgt cgcagtagca
    22681 tgcccactta caggaagtcc atccggcacg agcggcatag taaattcccc caccagttta
    22741 accggcggct gattttcaaa cgcgacgaca tccagttcgc tgactgtaag ttgttgccct
    22801 ttcagctggc cttgaaattt aactttttcg ccctgataac gcagttgctg gatatcagag
    22861 gttaatgcga gagagagttt tccctgccat tcctgccagg gagaaaaaat cagtttatcg
    22921 atattgatcc aggtgttagg cagcatggac tgccactgcg cgagggtttt tggagcagct
    22981 ggcgattgct ccgtctgcgg caatttcgcc agacaagcag aatcaagttc taccatgccg
    23041 acgttcaata accagcggct gggatgtgaa aggctggcgt tggtgatatg cgcaagctga
    23101 caatctccca ccagataacg gagatcggga atgattaaac ctttacgcgt aatgcgtggg
    23161 ctttcatcta atgcaatacg tgtcccgagc ggtagccaga tgcccgccag cgtgggaacc
    23221 cacagcccga gcgtcatcag cagcgtcaac ggcacaagaa taatcagtaa taacagcgcg
    23281 agaacggctt tatatttacc cagcatgggt agttaatatc ctgatttagc gaaaaattaa
    23341 gcattcaata cgggtattgt ggcatgttta accgttcagt tgaaggttgc gcctacacta
    23401 agcatagttg ttgatgaatt tttcaatatc gccatagctt tcaattatat ttgaaatttt
    23461 gtaaaatatt tttagtggct taaatgtgat tcaacatcac tggagaaagt cttatgaaac
    23521 tcgccgttta tagcacaaaa cagtacgaca agaagtacct gcaacaggtg aacgagtcct
    23581 ttggctttga gctggaattt tttgactttc tgctgacgga aaaaaccgct aaaactgcca
    23641 atggctgcga agcggtatgt attttcgtaa acgatgacgg cagccgcccg gtgctggaag
    23701 agctgaaaaa gcacggcgtt aaatatatcg ccctgcgctg tgccggtttc aataacgtcg
    23761 accttgacgc ggcaaaagaa ctggggctga aagtagtccg tgttccagcc tatgatccag
    23821 aggccgttgc tgaacacgcc atcggtatga tgatgacgct gaaccgccgt attcaccgcg
    23881 cgtatcagcg tacccgtgac gctaacttct ctctggaagg tctgaccggc tttactatgt
    23941 atggcaaaac ggcaggcgtt atcggtaccg gtaaaatcgg tgtggcgatg ctgcgcattc
    24001 tgaaaggttt tggtatgcgt ctgctggcgt tcgatccgta tccaagtgcg gcggcgctgg
    24061 aactcggtgt ggagtatgtc gatctgccaa ccctgttctc tgaatcagac gttatctctc
    24121 tgcactgccc gctgacaccg gaaaactacc atctgttgaa cgaagccgcc ttcgatcaga
    24181 tgaaaaatgg cgtgatgatc gtcaatacca gtcgcggtgc attgattgat tctcaggcgg
    24241 caattgaagc gctgaaaaat cagaaaattg gttcgttggg tatggacgta tatgagaacg
    24301 aacgcgatct gttctttgaa gataaatcca acgacgtaat tcaggatgac gtattccgtc
    24361 gcctgtctgc ctgccacaac gtgctgttta ccgggcatca ggcattcctg acagcagaag
    24421 ctctgaccag tatttctcag actacgctgc aaaacttaag caatctggaa aaaggcgaaa
    24481 cctgcccgaa cgaactggtt taatcttgcc gctcccctgc attccagggg agcttattca
    24541 gataatcccc aaagaccttt catcctctat tcttaaaata gccctgagtc agaaactgta
    24601 attgagaacc acaatgaaga aagtagccgc gcttgttgcg ctaagcctgc tgatggcggg
    24661 atgtgtaagt agtgacaaaa ttgctgtaac gccagaacag ttacagcatc atcgctttgt
    24721 gctggaaagc gtaaacggta agcccgtgac cagcgataaa aatccgccag aaatcagctt
    24781 tggtgaaaaa atgatgattt ccggcagcat gtgtaaccgc tttagcggtg aaggcaaact
    24841 gtctaatggt gaactgacag ccaaagggct ggcaatgacc cgtatgatgt gcgctaaccc
    24901 gcagcttaat gaactcgata acaccattag cgaaatgctg aaagaaggtg cacaagtgga
    24961 tctgaccgcg aaccagttaa cgctggcgac cgcgaaacag acattaactt ataagctggc
    25021 ggatttaatg aattaatagc tgccacagct cccggcggca agtgactgtt cactacagcg
    25081 tttgccgttg ggtaatgcac acatcccaat cgccgtacca tccagttgac gggcaacaga
    25141 aagcgaaccg ccgatcattg cacaatttgc ttctccacta ctggacatcg acgcttttaa
    25201 acctggcgct acgtgcgccg cagtcgcctg ctgaacaggc tcactactac acgccgacaa
    25261 caataaagcg gcacacccta cccaaaacgc tgctcgcatc ttttcttcct ctgatcttca
    25321 agccaaacga caccgccata aataataggc agcacagagg gcggcgtcga gagctgtcct
    25381 gcgcgttgcc ccgcattttt acttttttat ggctattttt ttgccctctg tttgatcaaa
    25441 acattcatta cgctgatgtg ggggacacaa aagcgaaaat gcagaagaaa gccatttgct
    25501 aaaattgaaa gattactact gggcgcgcag caatttcgtg cgcccctcat ttgcgcaatg
    25561 taagggtgtc atatgattac tattgacggt aatggcgcgg ttgcttcggt cgcatttcgc
    25621 accagtgaag ttatcgccat ctaccctatt acccccagtt ccacgatggc agaacaggct
    25681 gatgcctggg ccggaaacgg cttaaagaac gtttggggag acacaccacg cgtggttgaa
    25741 atgcagtcgg aagcgggtgc tatcgctacc gtgcatggcg ctttgcagac gggtgccctt
    25801 tcaacatcgt ttacgtcatc gcagggtttg ctgctgatga tcccgacgct gtacaaactg
    25861 gcaggcgaac taacaccgtt tgtcctgcat gtagcggcac gtaccgttgc cacacatgca
    25921 ctctctattt ttggcgatca ttccgacgtt atggcggtgc gccagacggg ttgcgcgatg
    25981 ttgtgtgcag caaacgtcca ggaagcgcaa gactttgctc tcatttcgca catcgcgacg
    26041 ctgaaaagcc gcgtgccatt tattcatttc tttgatggtt tccgcacgtc ccacgaaatc
    26101 aataaaattg tcccgctggc cgatgacacg attcttgatc tcatgccgca ggtcgaaatt
    26161 gatgctcatc gcgcccgggc actcaacccg gaacatccgg tgatccgcgg tacgtccgcc
    26221 aatcctgaca cttatttcca gtctcgcgaa gccaccaacc catggtacaa cgcggtctat
    26281 gaccatgttg aacaggcgat gaatgatttc tctgccgcga caggtcgtca gtatcagccg
    26341 tttgaatatt acgggcatcc gcaagcggaa cgggtgatta tcctgatggg ctctgccatt
    26401 ggcacctgtg aagaagtggt tgatgaattg ctaacccgtg gcgaaaaagt tggcgtgctg
    26461 aaagttcgcc tgtaccgccc cttctccgct aaacatttac tgcaagctct gccgggatcc
    26521 gtacgcagcg tggcggtact ggacagaacc aaagaacccg gtgcccaggc agaaccgctc
    26581 tatctggatg taatgaccgc actggcagaa gcctttaata atggcgagcg cgaaactctg
    26641 ccccgtgtca ttggtgggcg ctatggtctt tcatccaaag aatttggccc agactgtgta
    26701 ctggcggtat ttgccgagct caacgcggct aaaccgaaag cgcgctttac ggttggtatt
    26761 tacgatgatg tgaccaatct gtcactgccg ttgccggaaa acaccctgcc aaactcggcg
    26821 aaactggaag ccttgtttta tggccttggt agtgatggca gcgtttccgc gaccaaaaac
    26881 aatatcaaga ttatcggtaa ttccacgccg tggtacgcac agggctattt tgtttacgac
    26941 tccaaaaagg cgggcggcct gacggtttct caccttcgag tgagcgaaca gccgattcgt
    27001 tccgcttatc tcatttccca ggctgatttt gttggctgcc accagttgca gtttatcgat
    27061 aaatatcaga tggctgagcg tttaaaacct ggcggcattt tcctgctcaa cacgccgtac
    27121 agcgcagatg aagtgtggtc gcgcttgccg caagaagttc aggccgtgtt aaaccagaaa
    27181 aaagcgcgct tctatgtgat taacgcggcg aaaatcgccc gcgaatgtgg cctggcggcc
    27241 cgtattaata ccgtcatgca gatggctttt ttccatctga cgcaaattct gcctggcgat
    27301 agcgccctcg cagaattgca gggtgcgatt gccaaaagtt acagtagcaa aggccaggat
    27361 ctggtggaac gcaactggca ggctctggcg ctggcgcgtg aatccgtaga agaagttccg
    27421 ttgcaaccgg taaatccgca cagcgccaat cgaccgccag tggtttccga tgccgcccct
    27481 gatttcgtga aaaccgtaac cgctgcgatg ctcgccgggc ttggtgacgc cctccccgtt
    27541 tcggcgctgc cgccagacgg cacctggccg atgggcacta cgcgctggga aaaacgcaat
    27601 atcgccgaag agatccccat ctggaaagag gaactctgta cccaatgtaa ccactgcgtt
    27661 gccgcttgcc cacactcagc tattcgcgca aaagtggtgc cgcctgaagc gatggaaaac
    27721 gcccctgcca gcctgcattc gctggatgtg aaatcgcgtg atatgcgcgg gcagaaatat
    27781 gtcttgcagg tggcaccgga agattgcacc ggttgtaacc tgtgcgtcga agtttgcccg
    27841 gcgaaagacc gtcagaatcc agagattaaa gccatcaata tgatgtctcg cctggaacat
    27901 gtcgaagaag agaaaatcaa ttacgatttc ttcctcaacc tgccagaaat cgaccgtagc
    27961 aaactggaac gtattgatat tcgtacatcg cagctgatta caccgctgtt tgaatattca
    28021 ggtgcttgct ccggttgtgg cgagacgccg tatattaaat tactgactca gctctatggc
    28081 gaccggatgt tgatcgctaa cgccactggc tgttcttcaa tttatggcgg taacctgccc
    28141 tctacaccgt ataccaccga tgccaacggt cgtggaccgg catgggcgaa ctctctattt
    28201 gaagataatg ccgaatttgg ccttggtttc cgcctgacgg tcgatcaaca ccgtgtccgc
    28261 gtgctgcgtc tgctggatca atttgccgat aaaatcccgg cggaattact gacggcgttg
    28321 aaatcagacg ccacgccaga ggttcgtcgt gaacaggttg cagctttacg ccagcaactc
    28381 aacgatgttg ccgaagcaca tgaactgcta cgtgatgcag atgcactggt ggaaaaatca
    28441 atctggctga ttggtggtga tggctgggct tacgatatcg gctttggcgg tctggatcat
    28501 gtattgagtt tgacggaaaa cgtcaacatt ctggtgctgg atacgcaatg ctattccaac
    28561 accggtggtc aggcatcgaa agcgacaccg ctgggtgcag taactaaatt tggcgagcac
    28621 ggcaaacgta aagcgcgtaa agatcttggc gtcagtatga tgatgtacgg tcatgtttat
    28681 gtggcgcaga tttctctcgg cgcgcagctg aaccagacgg tgaaagcgat tcaggaagcg
    28741 gaagcgtatc cggggccatc gctgatcatt gcttatagcc cgtgtgaaga gcatggttac
    28801 gatctggcac tcagccacga ccagatgcgc caactcacag ctaccggctt ctggccgcta
    28861 tatcgctttg atccgcgtcg tgccgatgaa ggcaaactgc cgctggcctt ggattcacgc
    28921 ccgccgtcag aagcactgga agaaacgtta cttcacgagc aacgtttccg tcggctgaat
    28981 tcgcagcagc cagaagtggc agaacagtta tggaaagatg ctgcagctga tttgcaaaaa
    29041 cgctatgact tcctggcaca aatggccgga aaagcggaaa aaagcaacac cgattaaatg
    29101 ctcgggataa ggattatcca attctaaaaa aaaagcccgg acgactgttc gggcttgtct
    29161 ttttatatgt tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac
    29221 aataaaggca t
//
LOCUS       DAZGXZ010000058        29212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000058 DAZGXZ010000000
VERSION     DAZGXZ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.058"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(113..751)
                     /gene="msrA"
                     /locus_tag="ACHKJF_002625"
     CDS             complement(113..751)
                     /gene="msrA"
                     /locus_tag="ACHKJF_002625"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP0949963.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            957..2690
                     /gene="tamA"
                     /locus_tag="ACHKJF_002626"
     CDS             957..2690
                     /gene="tamA"
                     /locus_tag="ACHKJF_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP0949964.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            2687..6466
                     /gene="tamB"
                     /locus_tag="ACHKJF_002627"
     CDS             2687..6466
                     /gene="tamB"
                     /locus_tag="ACHKJF_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP0949965.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGILSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPQTTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            6469..6810
                     /gene="ytfP"
                     /locus_tag="ACHKJF_002628"
     CDS             6469..6810
                     /gene="ytfP"
                     /locus_tag="ACHKJF_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP0949966.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            7022..7273
                     /gene="chpS"
                     /locus_tag="ACHKJF_002629"
     CDS             7022..7273
                     /gene="chpS"
                     /locus_tag="ACHKJF_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP0949967.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSPPAGDEIW"
     gene            7267..7617
                     /gene="chpB"
                     /locus_tag="ACHKJF_002630"
     CDS             7267..7617
                     /gene="chpB"
                     /locus_tag="ACHKJF_002630"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP0949968.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(7697..8227)
                     /gene="ppa"
                     /locus_tag="ACHKJF_002631"
     CDS             complement(7697..8227)
                     /gene="ppa"
                     /locus_tag="ACHKJF_002631"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP0949969.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            8537..9493
                     /gene="ytfQ"
                     /locus_tag="ACHKJF_002632"
     CDS             8537..9493
                     /gene="ytfQ"
                     /locus_tag="ACHKJF_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP0949970.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            9803..11305
                     /gene="ytfR"
                     /locus_tag="ACHKJF_002633"
     CDS             9803..11305
                     /gene="ytfR"
                     /locus_tag="ACHKJF_002633"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP0949971.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            11319..12341
                     /gene="ytfT"
                     /locus_tag="ACHKJF_002634"
     CDS             11319..12341
                     /gene="ytfT"
                     /locus_tag="ACHKJF_002634"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP0949972.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            12328..13323
                     /gene="yjfF"
                     /locus_tag="ACHKJF_002635"
     CDS             12328..13323
                     /gene="yjfF"
                     /locus_tag="ACHKJF_002635"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP0949973.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(13356..14354)
                     /gene="fbp"
                     /locus_tag="ACHKJF_002636"
     CDS             complement(13356..14354)
                     /gene="fbp"
                     /locus_tag="ACHKJF_002636"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP0949974.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            14530..15903
                     /gene="mpl"
                     /locus_tag="ACHKJF_002637"
     CDS             14530..15903
                     /gene="mpl"
                     /locus_tag="ACHKJF_002637"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP0949975.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(16054..16605)
                     /gene="yjgA"
                     /locus_tag="ACHKJF_002638"
     CDS             complement(16054..16605)
                     /gene="yjgA"
                     /locus_tag="ACHKJF_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP0949976.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            16699..18051
                     /gene="pmbA"
                     /locus_tag="ACHKJF_002639"
     CDS             16699..18051
                     /gene="pmbA"
                     /locus_tag="ACHKJF_002639"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP0949977.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            18234..18620
                     /gene="cybC"
                     /locus_tag="ACHKJF_002640"
     CDS             18234..18620
                     /gene="cybC"
                     /locus_tag="ACHKJF_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP0949978.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(18665..19129)
                     /gene="nrdG"
                     /locus_tag="ACHKJF_002641"
     CDS             complement(18665..19129)
                     /gene="nrdG"
                     /locus_tag="ACHKJF_002641"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP0949979.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(19287..21425)
                     /gene="nrdD"
                     /locus_tag="ACHKJF_002642"
     CDS             complement(19287..21425)
                     /gene="nrdD"
                     /locus_tag="ACHKJF_002642"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP0949980.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(21819..23474)
                     /gene="treC"
                     /locus_tag="ACHKJF_002643"
     CDS             complement(21819..23474)
                     /gene="treC"
                     /locus_tag="ACHKJF_002643"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP0949981.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(23524..24945)
                     /gene="treB"
                     /locus_tag="ACHKJF_002644"
     CDS             complement(23524..24945)
                     /gene="treB"
                     /locus_tag="ACHKJF_002644"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP0949982.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(25064..26011)
                     /gene="treR"
                     /locus_tag="ACHKJF_002645"
     CDS             complement(25064..26011)
                     /gene="treR"
                     /locus_tag="ACHKJF_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP0949983.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            26196..26249
                     /gene="mgtL"
                     /locus_tag="ACHKJF_002646"
     CDS             26196..26249
                     /gene="mgtL"
                     /locus_tag="ACHKJF_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP0949984.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            26390..29086
                     /gene="mgtA"
                     /locus_tag="ACHKJF_002647"
     CDS             26390..29086
                     /gene="mgtA"
                     /locus_tag="ACHKJF_002647"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP0949985.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
BASE COUNT         6847 a         7404 c         8067 g         6894 t
ORIGIN      
        1 tccttgcaat atattgaatt tggacgattt tgtaggcctg gtaaggcgtt tacgccgcat
       61 ctggcataaa taaagcgcac tttatcaaca atctacattt gtacccgtaa cgctatgctt
      121 ccggtggcag acagacgcca attccgccaa ttccacagta accatacggg tttttatgca
      181 gatattgctg gtggtcatct tcggcataat aaaacggtgt ggcgttagcg atttccgtgg
      241 tgatgtgacg atcatcatcg gcggcaagca tcgccgcctg aaaacgttcc agactggcgc
      301 gagctgcggc atcctgttct ggggtcagcg gataaatcgc tgaacgatac tgcgtgccgt
      361 ggtcattgcc ctgacgcatt ccctgggcgg gatcgtgatt ctcccaaaat acctgtagca
      421 actgctcata gctgatgacg gaaggatcgt aaacaatgcg taccgcttcg gcatgacccg
      481 tatcaccgga gcacacttcc cgataagtcg gatttggcgt atagcctccg gtatagcctg
      541 cggcggtgct gtaaacgccg ggtaactgcc agaacagacg ctccacaccc cagaaacaac
      601 ccatcgcaaa aatggcaatc tccattccgt caggtacatt ggtcattgag tgaccgttga
      661 ccgcatgcag cgtggctacg ggcatcgggg tgttacgtcc aggcagggca tcggcggggg
      721 aaaccagatg ctttttatca aataaactca tggtgtcgct ctcccgaaaa tcggtcattg
      781 gggttaaggt tgtaacaaga ggcgtatttg cacacaataa ccaccgtgaa taggtctaaa
      841 gtaaaacata agaaatattt ggggtttagt ctgcttttta atccatatta ctggattttt
      901 gttaagccgt ttaacggcgt tccaggggca ggaaaaaagg atattcagga gaaaatgtgc
      961 gctatatccg acagttatgc tgtgtaagct tactctgctt aagcggatct gccgtcgccg
     1021 cgaacgtccg tctacaggtc gaggggttat cgggacagct ggaaaagaac gttcgtgcgc
     1081 agctttctac gattgaaagt gatgaagtga cgccagaccg tcgctttcgc gcacgcgtcg
     1141 atgatgccat ccgtgaaggt ctgaaagcgc tgggttatta ccagccgacc attgaatttg
     1201 atctccgtcc accgccaaag aaagggcggc aggtattgat cgccaaagtc acgccaggtg
     1261 tgccggtgtt aattggcggc accgatgtgg tattgcgcgg cggcgcgcgg accgataaag
     1321 actatttgaa attgctcgat actcgcccgg ctattggcac ggtgctgaac cagggcgatt
     1381 atgaaaattt caaaaagtcc ttaaccagca ttgcgttgcg taaaggttat ttcgatagcg
     1441 aatttaccaa agcgcagctg ggcattgcgc tcggcctgca taaagccttc tgggatattg
     1501 attataacag tggcgaacgt taccgctttg ggcatgtgac ctttgaagga tcacaaatcc
     1561 gcgatgaata cctgcaaaat ctggtgccgt ttaaagaggg cgatgagtac gaatcgaaag
     1621 atctggcgga actgaaccgc cgactttctg ctaccggctg gtttaactcg gtggtggtgg
     1681 ctccacaatt tgataaagcg cgcgaaacga aagtattacc cttgacgggc gtggtttcgc
     1741 cgcgaactga aaacaccatc gaaaccgggg tcggttactc tacggacgtg ggaccgcgcg
     1801 tgaaagcgac gtggaaaaag ccgtggatga actcttatgg tcacagtctg accaccagta
     1861 ccagtatttc cgcgccggaa cagaccctcg acttcagcta taaaatgccg ctgctgaaga
     1921 atccactgga acaatattat ttggtgcagg gcggttttaa gcgcactgac ctgaacgata
     1981 ccgaatctga ctccactacg ctggtggctt ctcgctactg ggatctctcc agcggctggc
     2041 agcgtgccat taacctgcgc tggagcctcg accactttac ccagggtgaa attaccaaca
     2101 ccacgatgct gttttatcct ggggtgatga ttagccgcac gcgttctcgt ggtggcctga
     2161 tgccaacctg gggcgactcg caacgctact ctatcgacta ctccaacacg gcctggggct
     2221 cagatgtcga tttctccgtt ttccaggcgc agaacgtctg gatccgcaca ctgtacgatc
     2281 gccatcgttt tgtgacacgc ggcacgctgg gctggattga aacgggtgat ttcgacaaag
     2341 taccgccgga tctgcgtttc ttcgccgggg gcgatcgcag tattcgcggc tacaaataca
     2401 aatctatcgc cccgaaatac gccaacggtg acctgaaagg ggcctcgaag ctgataaccg
     2461 gatcgctgga ataccagtac aacgtgaccg gaaaatggtg gggcgcggtg tttgtcgata
     2521 gtggcgaagc ggtaagcgat attcgccgca gcgactttaa aaccggtacc ggggtcggcg
     2581 tacgctggga atcgccggtc gggccaatca aactcgattt tgccgtaccg gtcgcggata
     2641 aagacgagca cgggttacag ttttacatcg gtctggggcc agaattatga gtttatggaa
     2701 aaaaatcagc ctcggcgtgg ttatcgttat cttactgttg ctgggatcgg tggcgtttct
     2761 ggtgggcacc accagcggcc tgcatctggt atttaaagcg gcggatcgct gggtgccagg
     2821 actggatatt ggcaaggtca ccggcggctg gcgcgatctc accttgtctg acgttcgtta
     2881 tgagcagcca ggcgtggcgg taaaagcggg caatctgcat ctggcggtcg ggcttgagtg
     2941 cctgtggaac agtagcgttt gtattaatga cctggcgctg aaagacattc aggtcaacat
     3001 cgacagtaaa aaaatgcctc cttctgaaca ggttgaagaa gaggaagata gcggtccgct
     3061 ggatctctcc acgccgtatc ccatcaccct gacacgggtg gcgctggaca acgtcaacat
     3121 caagattgat gacaccacgg tgtcggtgat ggacttcacc tccggcctga actggcagga
     3181 gaaaaccctg accctgaaac cgacgtcgct gaaaggcctg ctgattgctc tgccgaaagt
     3241 ggcggaagtg gcgcaggaag aagtggtcga accgaaaatt gaaaatccgc agccggagga
     3301 aaagccgctc ggcgaaacgc tgaaagatct cttttctcgc ccggtattgc cggaaatgac
     3361 cgacgtgcat ttgccgctta acctgaacat tgaagagttt aaaggcgagc agttacgcgt
     3421 gacgggcgac acggacatca ccgtgcgcac catgctgctg aaagtgagca gcattgacgg
     3481 caatactaaa ctggacgccc tggatatcga ttccagtcaa gggatcgtca acgccagcgg
     3541 cacggcgcag ctgtcagaca actggccggt ggatatcacc ctcaacagca cactaaacgt
     3601 ggagccgttg aaaggagaaa aactgaagct gaaagtgggc ggtgcgctgc gcgaacagct
     3661 ggagattggc gttaaccttt ccggtccggt ggatatggat ttacgcgccc agacgcgact
     3721 ggcggaagcc ggattgccgc tcaacgtgga agtgaacagc aaacagcttt actggccgtt
     3781 caccggtgag aagcagtatc aggcggatga tctgaaactg aaacttaccg gcaaaatgac
     3841 cgattacacg ctctctatgc gtacggcagt gaagggactg gagatcccgc cagccaccat
     3901 tacccttgat gccaaaggta atgaacagca ggtcaatctc gacaaactca ccgtcgcggc
     3961 gctggaaggg aaaactgaac tcaaggcgtt gctcgactgg cagcaggcca ttagttggcg
     4021 cggtgagcta acgcttaacg gcattaacac cgccaaagag atcccagagt ggccgtcgaa
     4081 actcaatggc ttgattaaaa cccgcggtag cctgtacggc ggcacctggc agatggaggt
     4141 gccagaactg aagctgaccg gtaacgtcaa acagaacaaa gtgaacgttg acggcacgct
     4201 gaaaggcaac agttatatgc agtggatgat cccagggctt catctggaac tggggccaaa
     4261 cagtgccgaa gtgaaaggcg agctgggggt aaaagatctc aatcttgatg ccaccatcaa
     4321 cgcgccgggg ctggataacg cgctgccggg gcttggcggt acagcgaaag ggctggtgaa
     4381 agtacgcggc acggtggaag cgccacaact actggcggat atcaccgcgc gcggcctgcg
     4441 ctggcaggaa ctttccgtgg cgcaggttcg cgtggaaggc gacattaaat ccaccgatca
     4501 gatcgccggg aaactcgacg tacgcgttga gcaaatttcg cagccggatg taaatatcaa
     4561 cctcgtcacc ctgaatgcca aaggcagcga aaagcagcac gagctacagt tgcggattca
     4621 gggcgagcct gtctccgggc agcttaatct ggcaggaagt tttgatcgca aagaagaacg
     4681 ctggaaggga attcttagca atacccgctt ccagacgccg gtcggcccgt ggtcgctgac
     4741 ccgcgatatt gcgctggatt accgcaataa ggagcaaaaa atcagcatcg ggccacactg
     4801 ttggcttaac ccgaatgcgg agctgtgtgt gccgcaaact atcgatgcgg gggccgaagg
     4861 gcgtgcggtg gtgaatctca accgcttcga cctcgccatg ctgaaaccgt ttatgccgca
     4921 aaccactcag gccagcggta tcttcacggg taaagcggat gttgcctggg acaccacgaa
     4981 agaggggctg ccgcagggca gtatcaccct ttcggggcgt aacgtgcagg taacgcaaac
     5041 cgtcaacgat gcggcgctgc cggtggcgtt tcagacactg aatctgacgg cggaattgcg
     5101 taacaaccgt gccgaattgg gctggaccat ccgcctgacc aataacggcc agtttgatgg
     5161 acaggtgcag gtgaccgatc cgcaaggccg ccgtaatctt ggtggcaacg tcaatatccg
     5221 taacttcaac cttgcgatga taaaccccat ctttacccgt ggggaaaaag cagcggggat
     5281 ggtgagtgcc aacttgcgtc tgggtggtga tgtgcaaagc ccgcagttgt ttggtcagct
     5341 tcaggttacg ggtgtggata tcgacggcaa ctttatgccg tttgatatgc agccgagcca
     5401 gcttgcggtc aactttaacg gtatgcgctc gacgcttgcc ggtacagtac ggacccagca
     5461 gggtgaaatc tacctgaacg gtgatgccga ctggagccaa attgaaaact ggcgggcacg
     5521 agtaacggcg aaaggcagta aagtgcggat caccgtgccg ccgatggtac gaatggatgt
     5581 atcgccagat gttgtattcg aggctacacc aaacctgttt accctcgatg gtcgcgtgga
     5641 tgtcccgtgg gcgcgcatcg tggtgcacga tctgccggaa agcgcagtag gcgtctccag
     5701 cgatgtggtg atgcttaacg ataacctgca accggaagag ccgaaaacgg cgtcgattcc
     5761 gattaacagt aacctgattg tccacgttgg caacaatgtg cgcattgacg cctttggcct
     5821 gaaagcgcgg ctgacgggcg atcttaacgt cgtacaggac aaacaagggc tgggcctgaa
     5881 cgggcagatc aacatccctg aagggcgctt ccatgcctat ggtcaggatc tgattgtgcg
     5941 taagggtgag ttactgttct ctggtccgcc ggatcaaccg tatcttaata ttgaagctat
     6001 tcgtaacccg gatgctacag aagacgacgt aatcgccgga gttcgcgtca ctggtctggc
     6061 ggacgaaccg aaagcggaga tcttctctga cccggcgatg tcgcaacaag ctgcattgtc
     6121 ttatttgcta cgtggacaag ggctggagag cgatcagagc gacagtgcgg caatgacctc
     6181 gatgctgatt ggtctggggg ttgcgcaaag tggccagatt gtgggtaaaa tcggcgagac
     6241 gtttggcgta agcaatttag cgctcgacac ccagggagta ggcgactcct cccaggtagt
     6301 agtcagcggc tatgtattgc caggtctgca agtgaaatac ggcgtgggta tatttgactc
     6361 tatagcaaca ctcacgttac gttatcgcct gatgcctaag ctatatctgg aagccgtgtc
     6421 tggtgtagac caggcactgg atttgctcta tcagttcgag ttttagcaat gcgaatattt
     6481 gtctacggca gtttacgcca caaacaaggc aacagtcact ggatgaccaa tgcccagtta
     6541 ctgggcgatt tcagtatcga taactaccag ttgtatagcc tgggccacta tccaggcgca
     6601 gttccgggga acggaacagt acacggtgaa gtttatcgta ttgacaacgc cacgctggcc
     6661 gaacttgatg ccttgcgcac caggggcggt gaatacgcgc gccagttgat tcagacgccg
     6721 tacgggagtg catggatgta cgtttatcaa cgacccgtcg atggattaaa gctaattgaa
     6781 agcggcgact ggttagacag ggataagtaa ccatatgcat acgccacctt cgggtggcgt
     6841 tgttttttgc gagacgactc gcattctgtt ttgtaattcc ctcacccttt gcttttctct
     6901 ccgagccgct ttccatatct gttaacgcat aaaaaactct gctggcgttc acaaatgcgc
     6961 aggggtaaaa cgtttcctgt agcaccgtga gttatacttt gtataactta aggaggtgca
     7021 gatgcgtatt accataaaaa gatgggggaa cagtgcaggt atggtcattc ccaatatcgt
     7081 aatgaaagaa cttaacttac agccggggca gagcgtggag gcgcaagtga gcaacaatca
     7141 actgattctg acacccatct ccaggcgcta ctcgcttgat gaactgctgg cacagtgtga
     7201 catgaacgcc gcggaactta gcgagcagga tgtctggggt aaatcccccc ctgcgggtga
     7261 cgaaatatgg taaagaaaag tgaatttgaa cggggagaca ttgtgctggt tggctttgat
     7321 ccagcaagcg gccatgaaca gcaaggtgct ggtcgacctg cgcttgtgct ctccgttcaa
     7381 gcctttaatc aactgggaat gacgctggtg gcccccatta cgcagggcgg aaattttgcc
     7441 cgttatgccg gatttagcgt tcctttacat tgcgaagaag gcgatgtgca cggcgtggtg
     7501 ctggtgaatc aggtgcggat gatggatcta cgcgcccggc tggcaaagcg tattggtctg
     7561 gctgcggatg aggtggtgga agaggcgtta ttacgcttgc aggcggtggt ggaataaggt
     7621 gtgtttattt atcgcgggca taaaaaaacc cttactaacc gaagcccggt gttcagggtt
     7681 attgcgccag aagaacttat ttattctttg cgcgctcgaa ggaggcaacg atttcagctt
     7741 tagcggcttc tgcgttttcc caaccttcaa ctttcaccca cttgcctttt tcgaggtctt
     7801 tgtagtgctc gaagaagtga gcgatctgcg ctttcagcag ctctggcaga tcgttaacgt
     7861 ctttaatgtg atcgtattct ttgctcagct tggtgtgcgg aacagcaatc agtttcgcat
     7921 cttcaccggc ttcgtcggtc attttcagaa cgccaaccgg acggcaacgg atcacagaac
     7981 ccggctgcag cgggtacgga gtcgggacca gtacgtcaac cgggtcaccg tccagagaca
     8041 gggtgtggtt gatgtaaccg tagttgcacg gatagaacat cgcggtggac atgaagcggt
     8101 caacgaacag tgcgccgctc tctttgtcga tttcgtattt gatcggatct gcgttagccg
     8161 ggatctcaat aaccacgtag atgtcttccg gcagatcttt acccgcaggg acgttgagta
     8221 agctcatgtc tgtttccttt aaaaatatgt ggcaaacaag tgccgagtat tatagccaac
     8281 tcgcgccgaa tgtcttcgct tgttttcgtc ttcattttcc tttttcgcca gttttaagac
     8341 ggtatgcaga gcaggaaaat ccataaccgt agccgcattt ttcatagtga gatgaaagcg
     8401 attacaaact tgtgattaac gttttattta cttttttgaa gtgtgatgta acgcaatccg
     8461 ttacataacg aattgtctat agttttttcg cgaacatctt ttcaccaata ataattaccc
     8521 cgacgaggac aaccctatgt ggaaacgctt acttgtagtc tctgcagtct cggcagccat
     8581 gtcgtctatg gcgttggccg ctccgttaac cgtaggattt tcgcaggtcg gatcggaatc
     8641 cggctggcgt gccgctgaaa ccaatgtggc gaaaagtgag gccgaaaaac gcggaattac
     8701 gttgaaaatt gccgatggtc aacaaaagca ggaaaaccag attaaagcgg tacgttcctt
     8761 cgttgcacaa ggggtggatg cgatctttat cgctccagtg gtagcgacgg gttgggagcc
     8821 ggtattaaaa gaggcgaaag atgccgaaat cccggtcttc ttgcttgacc gttccatcga
     8881 tgtgaaagac aaatctctct atatgaccac tgtcaccgcc gacaacatcc tcgaaggcaa
     8941 gttgattggt gactggctgg taaaagaagt gaatggcaaa ccatgcaacg tggtggagct
     9001 gcagggcacc gttggggcca gcgtcgccat tgaccgtaag aaaggctttg ccgaagccat
     9061 taagaatgcg ccaaatatca aaattatccg ctcgcagtca ggtgacttca cccgcagtaa
     9121 aggcaaagaa gtcatggaga gctttatcaa agcggaaaac aacggcaaaa acatctgcat
     9181 ggtttacgcc cataacgatg acatggtgat tggtgcaatt caggcaatta aagaagcggg
     9241 cctgaagccg ggtaaagata tcctcacggg ttccattgat ggcgtaccgg atatctacaa
     9301 agcgatgatt gatggcgaag cgaacgccag tgttgaactg acgccgaata tggcaggtcc
     9361 cgccttcgac gcgctggaga aatacaaaaa agacggcacc atgcctgaaa agctaacgct
     9421 gaccaaatcc accctttatc tgcctgatac cgcaaaagaa gagttagaga agaagaaaaa
     9481 tatggggtat tgagggtatc aatgcctgat gccgattcgt aggccggata aggcgctcgc
     9541 gccgcatccg gcggtggtgc gctgaagcct gatgcgacgc ttaccgcgtc ttatcatgcc
     9601 tacgagttcg gtgctctgtg taggccggat aaggcgttca cgccgcatcc ggcagtggtg
     9661 caacaccgga cttaacaccg tgcacaatct gtcccctcgc cccttcgggg agagggttag
     9721 ggtgaggggg acaaacggcg cagctcttaa catcaggcac gatctattcc cagactttta
     9781 gagcgtggct aagggggaaa ccatgacgac cgaccaacac caggaaatcc tccgcaccga
     9841 aggattaagt aaatttttcc ccggcgtcaa agcgttagac aacgttgatt tcagcctgcg
     9901 ccgtggcgaa atcatggcgc tgctcggtga aaacggggcg ggaaaatcga cgctaatcaa
     9961 agcattaacc ggtgtatacc acgccgatcg cggcaccatc tggctggaag gccaggctat
    10021 ctcaccgaaa aataccgccc atgcacaaca actcggcatc ggaaccgttt atcaggaagt
    10081 caacctgcta cccaatatgt cggtcgctga taatctattc ataggtcgcg aacccaaacg
    10141 tttcggcttt ctacgccgca aagagatgga aaagcgtgcc accgaactga tgacatctta
    10201 cggtttctcc ctcgacgtgc gtgaaccgct taaccgtttt tcggtggcga tgcagcaaat
    10261 cgtcgccatt tgccgggcta tcgatctctc cgccaaagtg ctgatcctcg atgaacccac
    10321 cgccagcctc gacacccagg aagtagagtt actgtttggc ctgatgcgtc agttgcgcga
    10381 tcgcggcgtc agcctgatct ttgtcactca ctttctcgat caggtctatc aggtcagcga
    10441 tcggatcacc gtcttacgca acggcagttt cgtaggctgt cgggaaacgc gcgagctacc
    10501 gcagatcgaa ctggtaaaaa tgatgctggg gcgcgagctg gatacccacg cgctacagcg
    10561 tgccgggcga acattgttga gcgacaaacc cgttgccgcg ttcaaaaatt acggcaaaaa
    10621 aggaacgatc gcaccgtttg atctcgaagt acgccccggc gagatcgtcg gcctggcagg
    10681 cttgctggga tccggacgta ccgaaaccgc cgaagtgatc ttcggtatca aacctgctga
    10741 cagcggcacg gcgttgatca aaggcaaaca gcaaaacctg cgatcgccac atcaggcttc
    10801 ggtactgggc atcggcttct gcccggaaga caggaaaacc gatggcatca tcgccgccgc
    10861 ctcggtacgg gaaaatatca tcctcgctct ccaggcccag cgcggctggc tacgacccat
    10921 ttcccgcaaa gagcagcaag agattgccga acgctttatc cgccagcttg gcattcgcac
    10981 accttcaact gaacaaccga ttgaatttct ctctggcggc aatcagcaaa aagtgttgct
    11041 ttcacgttgg ctactgaccc gaccgcaatt tctgatcctc gacgagccaa cccgcggcat
    11101 tgatgttggt gcccacgccg agatcatccg cctgattgaa acgctgtgtg ctgacggtct
    11161 ggcgctgttg gtgatctcct ccgaactgga agagctggtg ggctatgccg atcgggtgat
    11221 tatcatgcgc gatcgcaaac aggtggcgga gatcccgctg gcagagcttt ccgttccggc
    11281 gatcatgaac gccattgcgg cgtaaggaga acagtgtgat gcctcaatct ctcccggaca
    11341 ctacgccgcc gaaaaggcgc tttcgctggc caacgggaat gccgcagctg gcggcgctat
    11401 tgctggtgct gctggtcgat agcctggtag ccccgcattt ctggcaggtg gtgcttcagg
    11461 atgggcgttt gttcggtagc cctatcgaca ttcttaaccg tgcagctccc gttgcgttac
    11521 tggcgattgg tatgacgctg gtgatcgcca ccggtgggat tgatctctcc gtaggggcgg
    11581 tgatggctat cgccggagcc acaacggctg cgatgacggt cgcgggattc agcctgccga
    11641 ttgttttgtt aagcgccctg ggcaccggca tcctggcggg attgtggaac ggcatactgg
    11701 tagcgatcct caaaattcag ccgtttgtcg ccaccctgat cctgatggtc gccgggcgcg
    11761 gcgtggcgca actgatcacc gccgggcaga tcgtcacgtt taactcgccg gatctctcat
    11821 ggtttggcag cggatcgctg ttgttcctgc caacgccggt cattatcgcg gtgctgacgc
    11881 ttctcctgtt ctggctgttg acccgcaaaa cggcgctggg gatgtttatc gaagccgttg
    11941 gtatcaacat tcgggcggca aaaaatgccg gggtaaacac gcggatcatc gtcatgctca
    12001 cctacgtgtt gagcgggctg tgtgcggcga ttgcgggcat tatcgtggcg gcggatattc
    12061 gcggtgccga tgccaataac gccgggttat ggctggagct ggacgccatt cttgcggtgg
    12121 tgattggcgg cggatcgctg atgggcgggc gctttaacct gctgctctca gtcgtggggg
    12181 cgctgattat tcaggggatg aacaccggaa ttttactttc tggctttccg ccggagatga
    12241 accaggtcgt aaaagcggtg gtggtgcttt gtgtgctgat tgttcagtcg caacgcttta
    12301 tcagtctgat taaaggagta cgtagccatg ataaaacgta atttgccact gatgatcacc
    12361 atcggcgtct ttgtgctggg ctatttctac tgcctgacgc agtttcccgg ttttgcttcc
    12421 acaagagtga tctgcaatat cctgacagat aacgcctttc ttgggataat cgccgttggc
    12481 atgacctttg tgatcctctc tggcggtatc gatctctccg tcggttcggt gatcgccttt
    12541 actggcgtgt ttctggcaaa agtgattggc gatttcggcc tctcgccgct gctggcgttt
    12601 ccgctagtgc tggtgatggg ctgtgccttc ggcgcattta tggggcttct gatcgacgcc
    12661 ctgaagatcc cggcatttat cattacgctt gcggggatgt tctttttgcg cggcgtcagc
    12721 tatctcgttt cggaagagtc gattccgata aaccatccca tttatgacac gctctcaagc
    12781 cttgcgtgga aaatccccgg cggcggtcgc cttagtgcga tggggctgtt gatgctggcg
    12841 gtggtggtta tcggtatttt ccttgcgcat cgtacccgtt ttggcaatca ggtgtacgct
    12901 attggcggca acgcgacgtc ggcgaacctg atggggattt ctactcgcag caccactatt
    12961 cgcatttata tgctctccac cggactggca acactggcgg ggattgtctt ctcgatttat
    13021 acccaggccg gatatgcact ggcgggcgta ggtgtggaac tggacgccat cgcgtcggtg
    13081 gtgattggcg gtacgttgtt aagtggtggc gtcgggacgg tgcttgggac gctttttggc
    13141 gtggcaattc agggactgat tcagacttac ataaactttg atggcacgct gagttcctgg
    13201 tggacgaaaa tcgccatcgg tattttgttg tttattttta tagcattaca gcgtggatta
    13261 acggtgctgt gggagaatcg tcagagttcg ccagtgacaa gagtcaacat tgcgcagcaa
    13321 taaaaacgcc tctccgtgtg gagaggcgca ggagattacg cgtccgggaa ctcacggata
    13381 aagcgttcga catcttcaac catatggtcg ttgccgacaa agaacgaacg gcgctggtgc
    13441 agagtttccg ggatgatatc cagaatacgc tctttgccat cgctcgcttt accgcccgct
    13501 tgttccgcca ggaatgccat cgggttgcac tcataaagca aacgcagttt gccgtccggg
    13561 tggctggcgg tgcttgggta gagataaata ccgcctttca gcaggttacg gtggaaatcc
    13621 gcaaccagtg aaccgatata acgtgaggta tacgggcggt tggtggattt atcttcttcc
    13681 tggcagaatt taatgtactt cttcaccccg ttcggaaact taatgtagtt tccttcgttg
    13741 atggagtagg ttttgccttt ctccgggaag cgcatccgtt cctggcacag gcagaaaacg
    13801 cccagcgaag gatcgtaagt aaaggcgtga acaccgcatc cggtggtgta aaccagcatg
    13861 gtagaggagc cgtataccac gtaacctgcc gcaacctgtt tgttaccagg ctggaggaaa
    13921 tcttcttccg ttaccggcgt gccgacgggc gtaactcggc ggtagatgga gaaaatggta
    13981 ccgacagaga cgttaacatc gatgttggac gagccatcca gtgggtccat cagcaccacg
    14041 tattttgcat gttcacagcc ttcaaagaca acaatctcat cttcttcttc agaggcaatg
    14101 cctgcaacga tatcgcgtgc tttcagtgcg gctttcagtt tttcattagc gaacaagtcg
    14161 agtttttgct gaacctcgcc ctgcacgttc tcagcaccgc tggcacccag gatatcaacc
    14221 agtcctgctt tgttgatatc gcgatggata atcttggcgc ccagttttat tgccgacagc
    14281 aaagcagtga gctcaccggt agcatgagaa aactcgtgct gcttttcgac aataaattca
    14341 cctaacgttt tcataaaact ttccctgcaa tgtttatgga gtaaagcgac cgcaacaatc
    14401 ttaacaaata atctcaatgt tgcgctcagg tgaatcgcgc cagcaaatta cggattatcc
    14461 tgaaatgcgt ttctcacttg cccgacatat gcgtaaaatg agcggcagat taaaaaagga
    14521 tagtgacgta tgcgcattca tattttagga atttgtggca cgtttatggg cggtctggcg
    14581 atgctggcgc gccagttagg ccatgaagta acgggttcgg acgccaatgt gtatccgccg
    14641 atgagcacct tacttgagaa gcaaggcatt gagctgattc agggttacga tgccagccag
    14701 ctcgagccgc agccggatct ggtgattatt ggcaacgcca tgacccgtgg aaatccgtgt
    14761 gtggaagcgg tactggaaaa aaacatccct tatatgtcag gtccacagtg gctgcacgat
    14821 tttgtgctgc gtgaccgctg ggtgctggcc gttgccggta cacatggcaa aaccaccacc
    14881 gcgggaatgg cgacctggat tctggaacag tgcggttaca aaccgggatt tgtgatcggc
    14941 ggtgtgccgg ggaactttga ggtttcggcg cgtctgggcg aaagcgactt ctttgttatc
    15001 gaagcggatg agtatgactg cgccttcttc gacaaacgct ctaaatttgt ccattactgc
    15061 ccgcgtacgc tgatcctcaa caaccttgag ttcgatcacg ccgatatctt tgacgacctg
    15121 aaagcgatcc agaaacagtt ccaccatctg gtgcgtatcg ttccggggca gggccgtatt
    15181 atctggccgg aaaatgacat caacctgaaa cagaccatgg cgatgggctg ctggagcgag
    15241 caggagctgg tgggtgagca ggggcactgg caggcgaaaa agctgaccac cgatgcttcc
    15301 gaatgggaag tcttgctgga tggcgaaaaa gtgggcgaag tgaaatggtc gctggtaggc
    15361 gaacataata tgcacaatgg cctgatggcg attgcagcgg ctcgccatgt tggtgtagcg
    15421 ccggcagatg ccgctaacgc gctgggttcg tttattaatg ctcgtcgccg tctggagttg
    15481 cgtggtgaag cgaatggcgt cacggtatat gacgattttg cccatcaccc gacggcgatt
    15541 ctggcaacgc tggcggcgct gcgtggcaaa gttggtggta cggcgcgcat tattgctgtg
    15601 ctggaaccgc gctcgaatac catgaaaatg gggatctgca aagacgatct ggcaccttca
    15661 ttaggtcgtg ccgatgaagt cttcctgctg caaccggcgc atattccgtg gcaggtggca
    15721 gaagtggcag aagcctgcgt tcagcctgca cactggagtg gcgatgtgga tacgctggca
    15781 gatatggtgg tgaaaaccgc tcagcctggc gaccatattc tggtgatgag caacggcggt
    15841 tttggtggga tccatcagaa actgctggat ggtctggcga agaaggcgga agctgcgcag
    15901 taattcggcc tcagcctgag atagcattgc cggataaggc gtttacgccg catccggcat
    15961 ttgagcatag tgcctgatgc gacgcttgat gcgtcttatc cggcctacag gtgcaccgca
    16021 tccggcaaac catactcaca tcaacaacga aaattaccct tcattctccg ccagttcgcg
    16081 cagatactgg aaaatctggc gtgcggattt cggcggctta ttcccttctt tctctttctt
    16141 cgcgttacgg atcagagtac gcagctgctg acgatcggca tcgggccaca gattcaatac
    16201 ttcagcgatg gcgtcatcac cttgatcgat cagacgatcg cgcaggtttt ccagtttatg
    16261 gaacagcacc acctgctggt tgtgacggtt tttcagctta tccagcgcct gacgaatagg
    16321 ctctacgtcg cgctggcgca gcatcttacc gatgagctgc aactggcggc ggcgaccttc
    16381 catcttaata cgctgggcga gctcaatagc cgcgcgcaga tccgcatcta acgggatctt
    16441 atccagcgcg tttttcccca gatcaacaat ttccgcgcca aggcgtttta gctcctcggc
    16501 atcacgttta atttcacttt tactgaccca gataatttca tcgtcttcgt cttcgatgtc
    16561 atcaccggga acgtcgtcga gccagtcttc gggctgctta gtcatctcag gctccttaaa
    16621 aaaagaggct aatgttacca gttaagatgc gcactgaaaa acggttctct gttagacttc
    16681 agagaaactc tctacattat ggcacttgca atgaaagtaa tctctcaagt tgaagcgcag
    16741 cgcaagattc tggaagaagc agtttccact gcgctggagt tggcctcagg caaatcggac
    16801 ggtgcggaag ttgccgtcag caagaccacc ggcattagcg taagcacgcg ttatggtgaa
    16861 gtggagaatg ttgaattcaa tagcgatggc gcgctgggga tcactgttta tcaccagaac
    16921 cgcaaaggta gcgcatcatc caccgattta agcccgcagg ccattgcccg caccgtacag
    16981 gcggcgctgg atattgcccg ttatacctcg ccagatccct gtgccggcgt ggcagacaaa
    17041 gagctgctgg cctttgacgc accagatctc gacttgttcc accctgcgga agtttccccg
    17101 gatgaagcca ttgaactggc ggcccgcgca gaacaggcgg cattgcaggc ggacaaacgc
    17161 attaccaata ccgaaggcgg cagttttaac agccactacg gtgtcaaagt ttttggcaac
    17221 agccacggca tgttgcaggg ttactgctct acgcgtcatt cactctccag ctgtgtaatt
    17281 gccgaagaaa atggcgatat ggagcgtgat tacgcctaca ccattggtcg tgcgatgagc
    17341 gatctgcaaa cgccagagtg ggttggggcc gactgtgctc gccgtacttt atcgcgcctg
    17401 tcaccgcgta aactctccac catgaaagcg ccagtcattt ttgccaatga agtggcaacc
    17461 gggctttttg gccatctggt gggggcgata gcgggtggct cggtttaccg taaatctacc
    17521 tttctgctgg attcgctggg taaacaaatt ctgccggact ggctgaccat tgaagagcat
    17581 ccgcatctgc tgaaagggct ggcgtcgacg ccattcgaca gcgaaggtgt gcgcaccgag
    17641 cgtcgcgata ttattaaaga tggcatcctg actcagtggc tgctgaccag ctactcggcg
    17701 cggaaactgg ggctgaaaag caccggacat gcgggcggta ttcacaactg gcggattgcc
    17761 ggacaagggc taagcttcga acaaatgctc aaagagatgg gcaccgggct ggtggtgacg
    17821 gagctgatgg gccagggcgt gagtgccatt accggtgatt attcccgtgg tgcagcgggc
    17881 ttctgggtag agaatggcga aattcagtat cccgttagtg aaatcaccat cgcaggtaat
    17941 ttaaaagata tgtggcgcaa tattgtcacc gtcggtaacg atattgaaac acgcagtaat
    18001 atacagtgtg gttctgtgct gttgccggag atgaaaatcg ccgggcagta aaaaactggc
    18061 gcgacctgcc gcgctataaa ctaaaaaatt acacaaaatc attcgcactg catcgagacg
    18121 gaaagtgaat gagggccggg gagcgtactc gcagtacgtg acccgggcga atgagcgtag
    18181 ccgtcgatga ggcagtgtga aggatgaagt gtaaataaaa aaggaagtga gcaatgcgta
    18241 aaagcctgtt agctattctt gcagtctcct cgttggtatt cagttctgcg tcgtttgccg
    18301 ctgatcttga agacaatatg gaaaccctca acgacaattt aaaagtgatc gaaaaagcgg
    18361 ataacgcggc gcaagtcaaa gacgcgttaa cgatgatgcg cgccgcagcg ctggatgcgc
    18421 aaaaagcaac gccgccgaag ctcgaagata aatcaccgga cagcccggaa atgaaagatt
    18481 tccgccacgg tttcgacatt ctggtcggtc agattgacga cgcgctgaag ctggcaaatg
    18541 aaggtaaagt aaaagaagcg caggctgctg cagagcaact gaaaacgacc cgcaacgcct
    18601 atcaccagaa gtatcgttaa ttcctcattt ccctgttgcc tgcactcagg taacagggaa
    18661 cttatcatcg caaatgatgc accacctgat tgctgctgcc gcgccagatc agggaagggt
    18721 cttttaaatc ctgcacaaat ttgccgtcga ccagcacgtt aatcaaatca acaacctgca
    18781 tttgcgcagc gttgagttcg tcgagtttat agcctgtcca cacccagatg tctttacccg
    18841 gacactcggc gcggatgcgt tttaccagtt tcagaatatc cggcacgttt tgcggatgca
    18901 gcggatcgcc gccggagagg gaaatcccct ggcgtttgat acgagtgtca ttcagatctt
    18961 taatgatctt gtcttccatt gctttggtaa atggctgacc agaatttacc cgccatgtgc
    19021 ttttgttata gcaaccgggg cattcatgaa cacacccgga gacaaacagg gtgcagcgag
    19081 tgccggggcc gttgacgatg tcgacaggat agtactgatg ataattcatt atttcgcctg
    19141 gcttatcagt ccgcgtgtag gctgcatccg gtaatcaatg cctgatgcga cgctgtcgcg
    19201 tcttatcagg cctacagccg ttgccaaatg taggccggat aaggcgttta cgccgcatcc
    19261 ggcatttgct tagcgcagag tgaagattaa cctatctgcc cattccccaa atgtttaacg
    19321 cggcgcttaa cttcttcctg cttaccagcg ttaaacggac gtgcatccgg gctacctaaa
    19381 tatccgcaca cgcggcgagt taccgacaca cgggaggcgt catggttacc acatttcggg
    19441 caagtgaagc ctttgctggt gcactcgaac tcaccggtaa agccacactc gtagcactca
    19501 tcaatcggtg tattggtgcc gtaatacggt acatgctgat agctgtaatc ccagacatct
    19561 tccagcgcct tcaggttgtg ctgaatgttt ggatactcgc cgtagcaaat gaaaccaccg
    19621 ttcgccagcg gcgggtaagg cgcttcaaag tcgatcttgt cgtacgggtt caccttcttc
    19681 tccacatcga ggtggaaact gttggtgtag taacctttat cggtcacgcc cggcaccacg
    19741 ccaaactcag cagtatcgag acggcagaag cggtcgcaca ggttttcact cggcgtgctg
    19801 tagagactga aaccataacc cgtttcttct ttccactgat ccactgcctg gcgcagacgt
    19861 tcaacaatgg cgataccttt cgcgcgaagc tgctcgttgt cgtaaacatg ctcgccgccg
    19921 aacagcgcgt taatggtttc gtggatgccg atgtaaccca gcgaaataga cgcacgaccg
    19981 tttttgaaga tttcagaaac atcatcgtca gcattcagac gtacgccaca agcaccttcc
    20041 atatagagga tcggggccac gcgcgctttc acgccttcga gacgagcgat acgggtcatc
    20101 agcgccttac gtgccagcac cagacgttca tccagcagct tccagaaggt ggcttcatcg
    20161 ccttttgctt ccagagcaat acgcggcagg ttcaggctga tcacgccgag gttgttacga
    20221 ccatcgtgga tctgctcgcc gttttcattt tcccacacgc cgaggaagct gcggcagccc
    20281 atcggggttt taaacgaacc ggtgactttc actacctgat cgtagttcag gatatccgga
    20341 tacatgcgct tgcttgcgca ctccagcgcc agctgtttga tgtcgtagtt cggatcgcct
    20401 tttttatggt tcagaccatc gcgaatcgca aacaccagtt tcgggaacac cgcagtttta
    20461 cggtttttac cgaggcctgc gatacggtta cgcaggatcg attcctgaat caggcgcgat
    20521 tcccagctgg tgcccagacc aaaaccaaag gttacaaacg gcgtctgacc gttggcggtg
    20581 tgcagggtgt ttacttcgta ctccagcgac tggaaggcat cgtagcactc tttgatggta
    20641 cgagagtttg cgtagccttc ggcgtccggg atgttccact cttctgcggt cttgcgatgt
    20701 ttgttgtagc tggcggtaac aaacggcgcc agcacttcat cgatacggtt aatggtggtg
    20761 ccgccataaa tatggctggc gacctgagca ataatctgcg cagttaccgc ggttgccgta
    20821 gagatcgact tcggcggttc aatctcggcg ttccccattt taaagccctg ggtcagcatg
    20881 cctttcaggt cgatcaacat gcagttgaac atcgggaaga acggcgagta atcgagatcg
    20941 tgatagtgaa tatcgccacg ctcatgtgcc tgcaccacgt cacgcggcag caggtgctgg
    21001 cgtgcatagt gtttagccac gatcccggcc agcaggtcgc gctgggttgg aatcaccttg
    21061 ctgtctttgt tggcgttttc gttgagtaac gaggcgttgg tctgctcgac cagaccacgg
    21121 atctcctggt tcaggcgacc gcgtttttca cgttcaatgt cgcgatcgtg acggtactcg
    21181 atgtaagcac gagccagttg tttgtatgga cccgacatca gctggttttc aactgcggtc
    21241 tggatctcgt tgatatccac ctggttgcgg ccctgcatct gctcgctgac aaccgcggca
    21301 acagtggcgc aataatcggc atcatcgact tccgctgctt tagctgcacg cagaatcgct
    21361 tctttgatgc gctctgattt aaacggcact ttgcagccgt ctcgtttcat cacatgcggt
    21421 gtcatgatcg ctccatattt ttaagaacag gttatccaca gaaattggga aaggcgtttt
    21481 ccggtttttt cgtttcgttt tccggtgctt tccgcaatcg ccatccgctt atccacaacg
    21541 ccgcaccttt ttcgtgggga gctgtagtag caattatagt cgattaatac aacatattgg
    21601 gttgggacgc attttaaagt ctatatatag tgctttgcat caaggatgtt tgaacttttt
    21661 ttgatgtagc tcaaagtaaa aagcagagcg tacggatgac gggcgctaca gcgttatgta
    21721 aattttttaa tgaatttgct ggttgaaaaa tcaacaaaaa caacatgctg acagacaaaa
    21781 ccccggaatg acaggcattc ccggggctta gcggatactt acttctgtaa ccaccagaca
    21841 gcctcaaaag gccgtaaagt catggcacag ggttgtggtg aggcttcttc gtagttatgc
    21901 atcacaagct gccagttgcc gcgcatttgc cctggctgcc agggttggat ctcacggcta
    21961 aggttggcaa tgaccagcaa ggtttgcccc ttccattcac ggcgatagca ccacaataca
    22021 gggctgtttg gcagcagatc ctggtaattg ccccatgtca ggatggcttc ttgcttacgc
    22081 agtgcgatta acttttggta ggtgtaaaac accgaggagt catcggccag cgcggcttct
    22141 acgttgattt gttgatagtt atcgcccagg ccaatccacg gttcgccagc cgtaaacccg
    22201 gcattatcgc cgttgctcca ttgcatgggc gtgcggctgt tgtcacggga tttactggcg
    22261 aggattgcca ataactcgtc ggcatcacga ccatcgttgc gcagctcggc aaacatattg
    22321 aggctctcca cgtcgcgata gtcagtaatg cgcgtgaaat gcgggttggt catgccaatc
    22381 tcttcgccct ggtagatata cggcgttccc tgcatgccat gcagcaccat cgccagcatt
    22441 tttgccgcag gcacgcggta ttcaccttca tcaccaaagc gagaaataat gcgcggctga
    22501 tcgtggttac accagaacaa ggcattccat gccacgttgt gcattccttg ttgccagtgg
    22561 cggaacaatg ttttcaacgc cacaaagtca ggtttagcca gcgtccattt ttcaccaccg
    22621 ggataatcga ccttcaggtg atgaaaatta aaggtcatcg acaattcact gcctgtcagt
    22681 gccgcgtatc gctggcaatg ctcaaggctg gtggaggaca tttcacctac ggtcattaac
    22741 ccgcgtggcg taaacacatc gcggttcatc tcgtgcaaaa actcgtgtgc tcgtggcccg
    22801 tcggtgtaaa agcgacgccc gtcgccatcc aggtcttcag ggaaacgcgg gtctttggag
    22861 atcagattca ccacatccag gcgcaacccg tcgaccccac gatcggccca gaattcacag
    22921 acttttttca gctctgcgcg taccgctgga ttctcccagt tgagatccgc ctgttctggt
    22981 gcaaagagat gcaaatagta ctgttcgctt tccgcatgcc agcgccacgc actaccgcca
    23041 aattttgaac gccagttgtt cggtggcgtt tctggttcgc catcgcgcca gatataaaac
    23101 tggcggtaag ggctttcttt gttcagcgcc tcgcgaaacc aggcatgttg ggtagaggta
    23161 tggttaaaca ccatatcgag aatgatacga atcccgcgcg attttgcctg cgtcaccagt
    23221 tcgtcaaaat cgtccagcgt gccgtaggtg ggatcaatcg ccgtatagtt cgctacgtcg
    23281 taaccgttat cgacctgggg agagatataa aagggggtta gccagatggc atcaacgccc
    23341 agtttatgca gatagtccag gcgttggata acgccacgta aatcgccggt gccgctaccc
    23401 gtggtgtcct gaaaactctt tggataaatc tggtagataa cgccgttttg ccaccagtgg
    23461 ggaagattag tcattacgtt attcctgcaa atgcgagggg gcgcaattgc gccccgaaga
    23521 aaattaaaca atatccagcg tgcccaggcg gtatttccgc tgatagataa acgaggtgag
    23581 tacaatcggg atgatgatgg cgataaccat tgccagcgca aatacctgcc agtagctcgg
    23641 ttgaatcgag agaattcccg gcaggccgcc tacgccgata ccattcgcca taacgccgtt
    23701 caggccgcat agtaatcctg ccagaccaga accaatcatc gcgcacagca tcgggaagcg
    23761 atatttcagg ttgatgccgt acattgcagg ctcagtgacc ccaagccagg cggagatagc
    23821 ggcaggcacg gagatctcgc gttcattgtg cttgcggctg gaaatgataa tgcctatcac
    23881 ggcggagccc tgagcgatat tcgacagcgc aatcagcggc cacactggcg tgccacccat
    23941 gctttgaatc atctgcaaat caatagcaag ggtggtctgg tgtacgccgg tgatcaccag
    24001 cggcgcgtac aggaagccaa acaatgccgc accaatcgga gcaaagctgc cggtcatcag
    24061 gtgacgtacc gcaaaggcaa cgccatcgcc aatcatgcga ccaaacggac caatcagcgc
    24121 atgggcgagg aacaccgcga ggatcagcga acagacgggc accaccacca gatagaggta
    24181 atccggtacg atgcgtttaa ggcgagtttc aataacgccc agcgccagcc cggctaacag
    24241 tgccgggatc acctgcgcct gatagcccac tttggcgatg ctgaacatgc caaagtccca
    24301 cacttccggc agctgctgcc cgagcagata agcgttcatc agctgggggg aaaccagtgt
    24361 cacgccaagc acgataccaa ggatcggcgt gccgcccatt tttttcaccg ctgaccagca
    24421 aatcccgacc ggcaggtaga agaagatcgc ttcaccgatc aaccacagaa aatcgtagat
    24481 cgtttgcagg gaagggtaca tttgcgccag cgtttgaccg ttgctcatgg gcaaatcgcc
    24541 gatcacatta cgaaaaccga ggatcaaacc gccgctaatc aacgcgggca gcaacgggaa
    24601 gaagatctcc gcgaaatgag agatcaactg ctcatgccat ttcatattct gccgggcggc
    24661 tttttttacc tgctctttat caacctgcgc ctgtccggtt gacgctatta gtgcttgata
    24721 gtaatcaccc acgttggtgc caatcaccac ctgaaattgc ccggcattgg tgaaacagcc
    24781 tttcaccatg gggagttgct caatttcttt cggtctggca ttggccggtt ggttgaggac
    24841 aaagcgcagg cgagtaatac agtggctcac cgtcgcaata ttgccgcgcc cgccgaccag
    24901 ttcaatcaac cgatcgatat ccgtttggtt tattttgctc atcataaagc cccatggcag
    24961 atgacatttt tggttggctg cagaatatat tgcgcggaaa aatttaaaaa cgggaacgtt
    25021 cccgaaacgc agcgaagatc acaatttatc gttcaggaaa cgatcaggac agggtggcgg
    25081 ggatgatgat ttgttgcggt tcgctgcgcc cggttacctg tgcgatcaat tggcaggccg
    25141 cctggcgtcc agcttcggcg taaccaggat ctacggtgac gatctccgga tgaaggaatt
    25201 tcattaatgg cgtattaccg acgctcgcca gttgcaaggt gtcgatgcgt tgctcttgca
    25261 ggtatttact tgcgccaagt gccagcgtgt cggttgcgca cagtaaggcg gtagtttcag
    25321 gcgtaatcac ttttgctacg ttctcatagc cttgcttcat agcaaggcct ggcagagcgg
    25381 caacgggatt cagtttatgc gctttgcaga acgccaggta ggcttcgtga cgtcgcttcc
    25441 cggtcgtcac gtcactatgc ggcacgccga gataactgat attacgatgc ccctggtcat
    25501 acagccgttg catcaggatc ttgattgccc cttcgtcgtc ataacagacc gaagcaaaac
    25561 cttttgcgtc acgcgccagc agaaccagcg atgactgcca gtgggctaac atttcttctg
    25621 ttatgccagt aaaaccgaac agcactacgc cgtcgatatt acgccgtttc agcaccccca
    25681 aatgttcggc aactaattgc ggggaaaact gactttccat catgattggg tcgtaacctt
    25741 gttcatagaa cgctggcagc atggtttgaa cggcgagatt ttctgacaac gaatccaggc
    25801 gggtaacaat gatggcgacc actttatcgc tttgcccacg catagcgcgc gcagagcggg
    25861 aaggggaaaa tccatgctga ttcatcactg cttcaacacg ctcgcgggtg cgctggctca
    25921 cgccgctttc gttattcagc acccgggaaa ctgtagattt ccccacgccg cttaagcgtg
    25981 cgatgtcttt gatggtcagc cgattttgca tcctgttgtc ctgtaacgtg ttgtttaatt
    26041 atttgagcct aactttaccc gtgcattcag caatgggtaa agtctggttt atcgttggtt
    26101 tagttgccag caggtattat atcgccatag atgctacgaa tattattgga ttctccttat
    26161 tatttgcggc gcttttttca cttaccggag gttatatgga acctgatccc acgcctctcc
    26221 ctcgacggag attaaaactt ttccggtaag cccgtctttt cacggcgtta ccggatgcgt
    26281 aaggccgtga cgttttaacg tccctgctca gctttattac cttcaggtaa ggcttcgcca
    26341 cgcctgaaga catttctgta ctgtttcaga cagtgcggag ggactcctta tgtttaaaga
    26401 aatttttacc cggctcattc gccatttacc ttcccgtctg gttcatcgcg atccattgcc
    26461 tggcgcgcag cagacagtga atacggcggt cccgccgtcc ttaagtgcgc attgcctgaa
    26521 aatggcggtg atgcccgaag aagaattatg gaaaacgttc gacacccatc cggaagggtt
    26581 aaatcaggcg gaagtggaat ctgcccgcga acaacatggt gaaaataaat tacccgcaca
    26641 acaaccgtcg ccgtggtggg tacatttatg ggtctgctat cgcaacccct ttaatatttt
    26701 actcaccatt ctcggcgcta tttcttacgc cacggaagat ttatttgccg cgggcgttat
    26761 cgcgctaatg gtcgctattt ctacgttgct gaactttatt caggaagcac gttccactaa
    26821 agcggcagat gccctgaaag cgatggtcag caatacagcg acggttctgc gcgtaattaa
    26881 cgacaaaggc gaaaatggct ggctggagat cccgatcgac cagctggtgc ccggcgatat
    26941 aataaaactg gcggcggggg atatgatccc ggcagattta cgtatcttgc aggcgcggga
    27001 tctgttcgtc gctcaggcgt cgttaaccgg tgagtctctg cccgtagaaa aagccgctac
    27061 cactcgccag ccggagcaca gcaatccgct ggagtgtgac accctgtgtt ttatgggcac
    27121 caccgtggtg agcggcacgg cacaagcaat ggtgattgct acaggtgcca atacctggtt
    27181 tggtcaactg gcggggcgtg ttagtgagca ggaaagcgag ccgaatgcct ttcagcaagg
    27241 gatcagccgc gtcagtatgc tgctgattcg ctttatgctg gtgatggctc cggtggtgct
    27301 gttaatcaat ggttacacca aaggcgactg gtgggaagcg gcactgtttg cgctttcggt
    27361 cgcggtaggc ttaacgccgg aaatgttgcc gatgattgtt acctcgacgc tggcgcgcgg
    27421 cgcagtaaag ctgtcgaaac agaaagtgat cgtcaaacat ctggatgcta ttcagaactt
    27481 tggcgcaatg gatattctgt gcactgataa aactggcacc ctgacacagg acaaaattgt
    27541 gctggagaat cataccgata tctccggtaa aaccagcgaa cgcgtgctgc atagcgcgtg
    27601 gttgaacagt cattatcaga ccggacttaa aaacctgctc gatacagcgg tgctcgaagg
    27661 tacggatgaa gagtcagcgc gctcgctggc cagtcgttgg caaaaaattg atgagattcc
    27721 gtttgatttc gagcgtcgcc ggatgtcggt ggtagtggca gaaaataccg agcaccatca
    27781 gctggtttgc aaaggtgcat tgcaggaaat cctcaatgtg tgttcgcagg tgcgtcacaa
    27841 tggcgagatt gtgccgctcg atgacatcat gctgcgtaag attaagcggg ttactgatac
    27901 gcttaatcgt caggggctgc gcgtggttgc ggtggcgacg aaatacctgc cagcgcgtga
    27961 aggagattac cagcgggcgg atgaatccga cctgatcctc gaaggatata ttgctttcct
    28021 tgatccgcca aaagagacaa ccgctccggc actgaaggca ttaaaagcga gtggaatcac
    28081 cgtaaaaatc cttactggcg acagtgagtt agttgcagcg aaagtgtgcc atgaagtggg
    28141 gctggacgcg ggagaggtgg tcattggtag tgatattgaa acgctatctg acgacgaact
    28201 ggcaaatctc gcacagcgca ccacgctgtt tgcccgcctg acgccgatgc ataaagaacg
    28261 cattgtgacc ttactgaagc gcgaagggca tgtggttggc tttatgggcg atggtattaa
    28321 tgatgcgccc gccttacgcg ctgcggatat cggcatttct gtggacggcg cggtagatat
    28381 tgcccgtgaa gcggctgata tcatcctgct ggaaaaaagc ctgatggtgc tggaagaggg
    28441 ggttattgaa ggacgtcgca ctttcgccaa catgctgaaa tacatcaaaa tgacggcgag
    28501 ctccaacttc ggtaatgtgt tcagcgtgct ggtggcgagt gctttcttgc ccttcctgcc
    28561 gatgttgccg ttacacttgc ttattcagaa cctgctgtat gatgtgtcac aggtggcgat
    28621 cccgtttgat aacgtcgacg acgagcaaat tcaaaagccg cagcgttgga atccggcgga
    28681 tctggggcgc tttatgatct tcttcggacc gatcagctcg atcttcgata ttctgacgtt
    28741 ttgcctgatg tggtgggtat tccatgccaa cacgccggaa acgcaaacgc tgttccagtc
    28801 gggatggttt gtggtgggct tactgtcgca aacgctgatt gtgcatatga tccgcacccg
    28861 ccgtgtgccg tttattcaga gctgtgcatc gtggccgtta atgatcatga ccgtgatcgt
    28921 gatgattgtc gggatcgcat tgccgttttc accgctggcc agttatctgc aactgcaggc
    28981 gctgccgtta agctatttcc cgtggctggt tgcgattctg gcagggtata tgacattaac
    29041 ccagttggtg aaaggatttt atagccgtcg ttacggctgg caataaagaa tcaactgggc
    29101 atggtggcct ggattcgaat tgcagacaaa gtgagttttg ttcatgccgg atgcggcgta
    29161 aatgccttat ccggcctaca aaatcgtgct aattcaaaat gttgcattat at
//
LOCUS       DAZGXZ010000059        28806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000059 DAZGXZ010000000
VERSION     DAZGXZ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.059"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..424)
                     /locus_tag="ACHKJF_002648"
     CDS             complement(<1..424)
                     /locus_tag="ACHKJF_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0949986.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSR"
     gene            432..937
                     /gene="tnpA"
                     /locus_tag="ACHKJF_002649"
                     /pseudo
     CDS             432..937
                     /gene="tnpA"
                     /locus_tag="ACHKJF_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(955..1623)
                     /gene="ydcL"
                     /locus_tag="ACHKJF_002650"
     CDS             complement(955..1623)
                     /gene="ydcL"
                     /locus_tag="ACHKJF_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP0949987.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1925..2518)
                     /gene="tehB"
                     /locus_tag="ACHKJF_002651"
     CDS             complement(1925..2518)
                     /gene="tehB"
                     /locus_tag="ACHKJF_002651"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP0949988.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2515..3507)
                     /gene="tehA"
                     /locus_tag="ACHKJF_002652"
     CDS             complement(2515..3507)
                     /gene="tehA"
                     /locus_tag="ACHKJF_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP0949989.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3631..4611
                     /gene="ydcK"
                     /locus_tag="ACHKJF_002653"
     CDS             3631..4611
                     /gene="ydcK"
                     /locus_tag="ACHKJF_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP0949990.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(4606..5142)
                     /gene="rimL"
                     /locus_tag="ACHKJF_002654"
     CDS             complement(4606..5142)
                     /gene="rimL"
                     /locus_tag="ACHKJF_002654"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP0949991.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLSKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5205..5429)
                     /gene="ydcH"
                     /locus_tag="ACHKJF_002655"
     CDS             complement(5205..5429)
                     /gene="ydcH"
                     /locus_tag="ACHKJF_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP0949992.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5569..7224)
                     /gene="opgD"
                     /locus_tag="ACHKJF_002656"
                     /gene_synonym="mdoD"
     CDS             complement(5569..7224)
                     /gene="opgD"
                     /locus_tag="ACHKJF_002656"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP0949993.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7449..8792)
                     /gene="ydcJ"
                     /locus_tag="ACHKJF_002657"
     CDS             complement(7449..8792)
                     /gene="ydcJ"
                     /locus_tag="ACHKJF_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0949994.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQARNHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            9009..9932
                     /gene="ydcI"
                     /locus_tag="ACHKJF_002658"
     CDS             9009..9932
                     /gene="ydcI"
                     /locus_tag="ACHKJF_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0949995.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9970..11610)
                     /gene="trg"
                     /locus_tag="ACHKJF_002659"
     CDS             complement(9970..11610)
                     /gene="trg"
                     /locus_tag="ACHKJF_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP0949996.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            12009..12158
                     /gene="hokB"
                     /locus_tag="ACHKJF_002660"
     CDS             12009..12158
                     /gene="hokB"
                     /locus_tag="ACHKJF_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP0949997.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(12136..12204)
                     /gene="yncP"
                     /locus_tag="ACHKJF_002661"
     CDS             complement(12136..12204)
                     /gene="yncP"
                     /locus_tag="ACHKJF_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP0949998.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(12230..12403)
                     /locus_tag="ACHKJF_002662"
     CDS             complement(12230..12403)
                     /locus_tag="ACHKJF_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0949999.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12648..13178)
                     /gene="cybB"
                     /locus_tag="ACHKJF_002663"
     CDS             complement(12648..13178)
                     /gene="cybB"
                     /locus_tag="ACHKJF_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP0950000.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13367..14368
                     /gene="gap"
                     /locus_tag="ACHKJF_002664"
     CDS             13367..14368
                     /gene="gap"
                     /locus_tag="ACHKJF_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0950001.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14410..15849)
                     /gene="aldA"
                     /locus_tag="ACHKJF_002665"
     CDS             complement(14410..15849)
                     /gene="aldA"
                     /locus_tag="ACHKJF_002665"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP0950002.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVDGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(16046..16846)
                     /gene="ydcF"
                     /locus_tag="ACHKJF_002666"
     CDS             complement(16046..16846)
                     /gene="ydcF"
                     /locus_tag="ACHKJF_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP0950003.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(17118..21020)
                     /gene="hrpA"
                     /locus_tag="ACHKJF_002667"
     CDS             complement(17118..21020)
                     /gene="hrpA"
                     /locus_tag="ACHKJF_002667"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP0950004.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            21221..21826
                     /gene="azoR"
                     /locus_tag="ACHKJF_002668"
     CDS             21221..21826
                     /gene="azoR"
                     /locus_tag="ACHKJF_002668"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP0950005.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVNFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21877..23193)
                     /gene="ynbD"
                     /locus_tag="ACHKJF_002669"
     CDS             complement(21877..23193)
                     /gene="ynbD"
                     /locus_tag="ACHKJF_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP0950006.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLKEDHKAMLKLWENR"
     gene            complement(23183..24940)
                     /gene="ynbC"
                     /locus_tag="ACHKJF_002670"
     CDS             complement(23183..24940)
                     /gene="ynbC"
                     /locus_tag="ACHKJF_002670"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP0950007.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHCGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYMPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGSVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSIGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24956..25852)
                     /gene="ynbB"
                     /locus_tag="ACHKJF_002671"
     CDS             complement(24956..25852)
                     /gene="ynbB"
                     /locus_tag="ACHKJF_002671"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0950008.1"
                     /translation="MLEKSLVTLFALLILATLINHFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLILATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25852..26457)
                     /gene="ynbA"
                     /locus_tag="ACHKJF_002672"
     CDS             complement(25852..26457)
                     /gene="ynbA"
                     /locus_tag="ACHKJF_002672"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0950009.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            complement(26628..28806)
                     /gene="ydbD"
                     /locus_tag="ACHKJF_002673"
                     /pseudo
     CDS             complement(26628..28806)
                     /gene="ydbD"
                     /locus_tag="ACHKJF_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
BASE COUNT         7497 a         7399 c         6636 g         7274 t
ORIGIN      
        1 gacggctgtt ccggtagcgc atccagccca gtttcggcag aaaaatacgg ctgttttcct
       61 gatcgagctt aacgccctgc gggtagcgga atacatcatt ctgtccccgc tttttgaatc
      121 ggggaaaagc agcccgattc tggaagaagt ttttgtaagc ccgctcaagg tctttcagtg
      181 actgttgcaa tggctgtgac ggggcatctt taagccattg cgtttcagtg gcatttttcc
      241 actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg acctcatgat
      301 tctcattctg acgtgccaga gcacgattga aaacgaaacg acatgcgcct gcgaagcgcc
      361 tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgct tgtagtcgtt
      421 tcacaggcca aatgatactt tggtctgtga aaaaagaaac cgatatccgg cgtggaaggc
      481 attgtgtttt cctgatgcat gttcacctgg tctttgtcac cagataccga cgtcagattt
      541 ttgaccatga cgcgacagaa aaactacgca cttacttttc aaatgtatgt gcttattttg
      601 aagctgaact ggttgaaatg gatggcgaac cagatcacgt ccatttgtta attaactaac
      661 ctcccaaact ggcgatatcc agtctggtaa acagcctcaa ggcgtatcgg gtaggttact
      721 gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt tgtggagtcc
      781 tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc aatacattga
      841 acaacagcaa acacctggtc aggtggaaaa ccgcgcctta tatccccgcc ctgaaggatg
      901 gggttttacg gcgcaccgga taaaaagcgc catctaacga tggcgcgtct ggaactactt
      961 tttgttaacg tcaaacatgg tggcatcggt cgccatgtca tcaataactt gtttaatatt
     1021 ttcgaaagcc attggcgtac tttcgttatt caggtcttta ccttcgccct gacgaacgac
     1081 tttgataacc ggtttattag tcgctgcatc aatcagctca ccttcaaaat agaggcgagt
     1141 atccatggta cggtggcctg tagccatttg cgtccccgca accactaatg caacaggaac
     1201 cacttcatag aattgcagcc cttctttgct ggtatctaca ccggtaatgg ccccacggaa
     1261 aatcagacta cgcggcccag cggtggtaac cactggttta cgctgcgcta tcgcttcttt
     1321 catttcggtg ttggtatagt tcaaaatttt atccagaact ttctgcccta cctgggtaga
     1381 aggttttggt accggataat aagtgattgg gttccagacg atgctgtcat atttgctttg
     1441 atcaaaactc gggtctaccc aacgtaaaac aggtttacct gttgctgagg ttgtttcttt
     1501 taaatcagag taattgttta aaaaaccaga atatttatct ggctgggtga ttttagaggc
     1561 acaaccagac agagccaaca agccgcataa agctgcaact ttcgcaaatg atgtggtacg
     1621 catgaatagt ttcctgagat gattatatta ctttctaagt tatagcaaaa agcgccgctt
     1681 cgtgttgtgg caaaaaaagt gttggtgaaa ataaattttt cagccccttt tcagacgaca
     1741 tagtcaatgc cgaaaaaggg gctaagtcaa tcatcagtag ttaaatttca gtaagttaag
     1801 ctgctaaaaa cttcgactac tgtgattaat ttcacatccc ttcgataaaa aatatgctta
     1861 ccgaaaagaa gaacagtaaa tcatctcagc gtggggaatt ttcacgcgtt ttgtgcttac
     1921 cgggtcattt tttacgtgcc agcatcgtgg cgaaacgcag tttaatacga ttaccgttgg
     1981 cgtcggtgcg gtgcagctcg ccgacgtctt cattgtattt caccctctcc cagccttcat
     2041 aatatcgacg taattctccc tctttgaagg caaacgggaa gccgacggta catggataat
     2101 cagcggtatc catcgccgcc acaatcaggt tgtaaccacc aggtttagtg caacgttgca
     2161 tattggcaat cagccctggg atggttttag cctcaaggaa catcagtacc acagtcgaaa
     2221 gaataaaatc gtactgtcta tcaaatgtga ggttattcag atcaacgacc cgggtgtgta
     2281 aattatccag attttcaatg gatttaatgc gctctacatt ggcgatactc atagcatttt
     2341 tatcccatgc gtcaacatca taaccattgg ctgccaggta aagactgtta cgaccattgc
     2401 cacagcccag atccagcgtt ttacccggtt taaccacttt caccgcttcc agtacttcag
     2461 agtgtgtgcg ggttaattca tatttatcag taaaatagtt ttcgtcacga atgatcattc
     2521 tttgtcctct gctttcatta aaacggcgcg ctcggttctg actaacaatt ttccctgcat
     2581 cagaagcgca aaagtacgga tgagcagtat tgcaataata aaattggtaa agataaacag
     2641 cggtaccgcc agcgtatgga aaaatccatt ttcgctgcca ctccccagat gcaaaccggt
     2701 ggttgccagt gcagatacgc cgaacgagaa actccagaat gaagcattaa atggctggga
     2761 gagataccat ggcatcagac gtagcataaa cagcagttgc agcagtccat aaccaaaaag
     2821 cattttcgcc agcgtatcac cctcgccgcc gttgacgctc aaccaggcac tacaggccac
     2881 cagcgcagga gcgagctgaa tgccgagtga tgtccgcagt gccgtgggta attctcccga
     2941 actgcgcaga cgctgcaaga tcacaggttc aaggcttagc catgagaaaa cgcctgcgcc
     3001 taaaaacacc agaccggcgt cggtgtagcc caacgcacca caggccattg cgctgataaa
     3061 gttgttggca actgtcggca gatacagtcc aggcgtggta gcttcttcag ggtgagatcc
     3121 gcgccataat cccgcagttt gccaggcggc ataagccaac tgaaccacga caccaaaact
     3181 gaacaggcac accgccagtg ggcgaaacca cggaacaaaa ccaatcgcca ccagcatcgt
     3241 cgttgccgga aacaaactca caaaactgct catcactgga tggcgcactt ctgccagcac
     3301 gctatgcgga aagcgtataa gtcgggtaat aaatgcgcta gttaacaatc cccagatgat
     3361 catcgccaga atcaccagcc catcccctaa ccagtggctg acctgccaaa cctggctggc
     3421 atagcgccag gcaaatccca tccctatcgt ccccaacaca ataccaaagt agcctgccgg
     3481 caaattgagc actttatcgc tctgcatctt cttactcatt ttgtttaatt tataaagtat
     3541 atttgaaatg cattttatgg aattttctcg agcatagcca gagccgcaga atttgctacg
     3601 gttatagtaa gtcacctgtg ggaaaacgct atgcgcaaat atcgtctaag tgaagaacag
     3661 cgagccttta gttatcagga agatggcact aaaaaaaatg tgttattacg gcagattatc
     3721 gccattagcg attttaacga tgtgatagca ggaaccgctg gcggctggat cgatcgcgaa
     3781 acggtgttag cacaggaagg aaattgctgg atttacgacc agaacgctat tgcgttcggc
     3841 ggagcggtga tttccggtaa tacgcgcatc accggcacca gcgtgctgtg gggagaggtt
     3901 tacgcaacgg ataatgtctg gatcgataat agtgaaatca gccagggtgc atatataagt
     3961 gacagcgtca ccattcatga ctcactggtt tgcgggcaat gtagaatatt tggtcacgcc
     4021 ttaattgatc aacattctat gatcgtcgcc gcacaaggct taacgcccga ccatcaactc
     4081 ctgctccaaa tttatgatcg cgcaagggtt agcgcctcgc gtattgtcca ccaggcacaa
     4141 atttatggcg atgccgtcgt ccggtatgct tttattgaac atcgcgccga agtttttgat
     4201 tttgccagtg ttgaaggtaa tgaagagaac aacgtctggc tatgcgattg cgctaaagtc
     4261 tacggtcacg cgcaggtgaa agctggcata gaagaagatg ccattccgac gattcattac
     4321 agttcgcagg tggcggaata tgccattgtg gaaggtaatt gtgtgttaaa acatcatgtc
     4381 ttgattggcg gaaatgccgt ggtacgtggt ggaccgattc tgctcgacga gcacgttgta
     4441 atacaaggtg agagccgtat ttcaggtgcg gtgataattg aaaaccatgt ggagttaacc
     4501 gaccacgccg tcgttgaagc ctttgatggc gacacggttc atgtccgtgg tccaaaagtc
     4561 attaacggcg aggagcgcat tacgcgaacg ccgctggcag ggttattatg aatcgataat
     4621 acgcgcgtat aagttcacat catcataggc atcattcagg aactcagcct gtttcaggca
     4681 accttcaagg ataaaaccat tgcgcaaagc gacctggttg ctttgcggat tgtccacccg
     4741 acatttgatc acgaagcgtc taagttcacc agactgagcg taatgatgaa tcaatgcctg
     4801 cagcgcctga gaaatgatcc cctgcccctg atgagattcg tccagccagt agccgatttc
     4861 agcagtttta ctcagtggtt caatacgatt aaacgagata acgccgataa gttcatcttc
     4921 tttgaaaatc atgaacattt tggcatagcc gcgttgatgc aacatcacat taccctgcac
     4981 cgtttttcgc gtgtcctctt cactctgaac gaactgcggc cagtttagcg actgctgtaa
     5041 ccaggtttta tttttacaga ttaactgata aagaggtgtg acgtgacttt ccgcaacagc
     5101 atgtaattca agtgattcgc ttacttttat cgtttcagtc atcttatctc cagtcaatcc
     5161 atgctgttgt taaaaaagcc gcctggctgg cggcttagga aagtttacac ctctttgacg
     5221 ctctcctgct gcaggatttt gagcatctca tcttttaact gtagcttttg ttttttcatg
     5281 cggaccactt ccgcattgta ccctcgaccg tcggaacctt cctttctggc aatttcatga
     5341 tcaagtttat tgtgtttatc gaacaaggac ataaagcgag gattttcgtt tttcagacgg
     5401 gatattaaat ctcggtattc tggaaacata cgcactccct gttagtggct gacaaggtgt
     5461 atctgaaata cacaccatca gattactcat ttaacttagg gaaaaatgcg agcgggatcg
     5521 tgtttaacca gacggcacct cccgaagaag gtgccgaaga aaaaacgatt aactcatcac
     5581 gcggtcgtca acatactgac gtttatccgg cgctggcggg aaatactgat acagccatgt
     5641 ttcactgata gcgtcgccct gacaacgcag atacatccgc atatcgaccg gatcagtgga
     5701 gtccgaagtc ggataccagt caaactgaat acgataacca tcaatgggtt caatatagag
     5761 aatttcgatt tgcttcgctt ccccactgga aagcgtaatc accggctcaa tgccttttgg
     5821 tgcggcagct ttcagatcac caccaacgaa atcgacggca aaacgacgcg cccatttttc
     5881 gggatagtgt tcacctggtg cccaaccttc cgggaaaccg cccatgccgg tacgcgtcgc
     5941 cataacgcgc gctaatgggc aatgaacagg cggttgcgca ctccagtaca gacgatactg
     6001 gaatgcaaac tcatcacccg cctttacagc tttttctggc tgccagaagc agacaatgtt
     6061 atccagcgtt tcgcccgttg ttgggatttc catcaggccg atggtgccct taccccactt
     6121 gttacgcggt tccacccaca gacttgggcg tttgttatac cagcccataa tgtcctgata
     6181 atgggagaaa tcacgatcca gttgcaataa accaaaccct ttcgggttgt tgtcggtgta
     6241 agcattgaac tgcaattttt gcggattatt cagcggacgg caaatccact cgccgttgcc
     6301 ccgccacatg gacagacgat cagagtcatg aatttgcgga tgaatggtgt cgcacatccg
     6361 acgttcatta gtaccgcagc tgaacatact ggtcatcggc gcaatgccca gctgtttaat
     6421 gtctttgcgc gcatacaggt gattttccac atccataatc acctgacttt tctcacaatg
     6481 gatagtgaac ttataggcac cagtaatgct ggcgctatcg agcaacgcat aaacggtaaa
     6541 ggtagttgcc cccggtttta ccgtatcaaa ccagaaggcg gtaaagtcgg ggaactcttc
     6601 tttactgtcg gtgtaagtgt caatcgccag gccgcgggcc gacaaaccgt attgatatgt
     6661 gtcatcaacg gcgcggaaat aactcgctcc gaggaatgat actacatcac ggcgcgccag
     6721 ttcgggggct ttaaacacgc gaaaaccggc aaagccgaga tcgctttgcc cttctaattg
     6781 tttggtatca acacctgcat cgttgtattt gaacaactcc gggcgaaagt gaatttcacg
     6841 cgccagatgt gttgctggat ctacagaaaa catacgaacg cggcgacgga atcccattcc
     6901 catatggaag aactgagcgt ccagttgacg gttctcaacg ttatgccaga gcgatttttc
     6961 ggcgtcgtat tgaatactgt tataagcctg cggcgtcatt gtcgccagcg tgtcaggtaa
     7021 cggacgaggc gcaccacgcc acgctgtttg cgctaagtcg tgcgccattg actgtagaat
     7081 ggagaagtca aaacgctggg tttgcccgtc ggcaatatca gaatctgccg cgaatgccgc
     7141 ctgagaaaaa agagaagcaa tgccgctggt accgcacacg gcggccatag ccattgaacc
     7201 tttaataaat cgtctacgat ccatacctga aagtgagtcc ttctggtgtt gtgaatggtc
     7261 ccgcgctggt cgttttcagc ggcaagaaag tgcttattta tgaccgctca ctctagacaa
     7321 tatttattaa taatccaatt gttgaactca gagaatatgc ataaatcggc aattttttta
     7381 tgtcgatggg atgagcctga ttcgtttgta aaatcaggca aatttttgca aaggcggatg
     7441 gtcagaattt aaagcaaacc acagcgacgt ttactgcgtt cttccgcttc ctgataaagc
     7501 tggaactcat ccaaaaccgg acaacccaac gcctgctcaa atgcctcgcg actggcatta
     7561 ccgtgattgc gtgcctgcgt ttcattaccc agatttgact ggaagatccc cgccgcgcta
     7621 acgggcaaga aatcttcata ggtaatgggt tgcgccgcga cccaaccacg ttcaattaag
     7681 ggctgtggat cgtctccagg atgaatcgcc tgacgatgcg cctcacctga aggcgtcaga
     7741 cggtaccgga accatgccag cccttgctga cgcattaaaa actcactgtc aggaaaagtg
     7801 cggaaggttt cctgtaaatg catttggtga gtgagattat cctgcccggt tccagcgtta
     7861 cgcagaagat catcatacag ttgtcgccct ttcggcgtta atgccacgcc acgctgctca
     7921 atttcaccaa agcgcgcggt atgcgtgccc tgtttctgcc ccgcaaacaa caccgtctct
     7981 tccagtgctt taaagctggt ctggcgtagt aaaatcggta cctcgcggcg cggcggcccc
     8041 tcgatgagaa ttttgggttc aattccgcat tcaggcatca tcgactgcac ccggtcaata
     8101 tccagcgtac gtggcgtcag gtggttgata tggcatccag gaaaacagac cacatcagca
     8161 atcaaccgat gttcgttgtg caaggcgcga taggtttctt catctaccgt tgctgactgg
     8221 tgccagcgaa acgtttccag ggcttcctgc acaaactcct gtgcctgtgt ttcgttaaaa
     8281 ccgccctgct gctcatattc ctctaacagt tgtcgacaac gtggggtgaa gatatcgcgc
     8341 tgacgcagaa tctccgccgc tttctggcgc aaaatttcgt tctcgataag ctcaaggcgg
     8401 agtaaggagg taaaaacgcg gaagggatta cgcgccagag aagcatcatc aatgggccga
     8461 aatgctgtcg aatgtaccgg cccccctgcc tgcgagagat cgtaatagct caccgggtac
     8521 atccccataa tggcaaacat ccgccgaaga gtagcaagct cttgtgcagt cccaacgcga
     8581 atcgccccat gacgttcaac attcagtcgc gccagctcgt ctgcatttac cattttttcg
     8641 tgcagttgag gattgttttc cagcacagcc agattcacat cagctaccag ttccagcagc
     8701 gtgccatatt gcggaacttc ttgctggtac atggctgaca ttgcctgcga aaactgttcc
     8761 cgaatctcat ccgccgtgat gctgttcgcc atagtgtctg cctccagtgg tcagtaatct
     8821 ggagtgtagg taaccgcatt cactctggcg ggaagaattt acaaactgtg atctcgccgc
     8881 gaaaacatca atattatcca ttttgctgta acataattgc tttaattgtt aataatattt
     8941 tgcaatcaag ttatcataat caaacaactt cacttgtcag cgacaccgct tcgtttttaa
     9001 catcgcttat ggaaaaaaat agtctgttta gtcagcgcat ccgtttgcgc caccttcata
     9061 cattcgtagc tgtcgcacaa caaggaactt tggggcgcgc ggctgaaacc cttaatttga
     9121 gtcaacctgc gctctctaag acattgaatg aactggagca gctgactggc gctcgcttgt
     9181 ttgagcgtgg tcgtcagggg gcgcaactta ccttacccgg cgaacaattt ttaacgcatg
     9241 cagtcagagt tcttgacgcc atcaacactg ccggacagtc gcttcatcgt aaagaagatc
     9301 ttaataatga tgtcgtcagg gttggtgcac tacctactgc ggcactgggg atattacctt
     9361 cggttatagg tcagtttcat cagcaacaaa aagagacgac cttgcaagtt gcgacaatga
     9421 gtaaccctat gattctggcg ggtttaaaaa ccggggaaat cgatatcggc attggtcgga
     9481 tgtcagatcc tgaactgatg accgggctta attacgaact gctgtttctt gaatcgctga
     9541 agctggttgt ccgccctaat cacccgctac ttcaggagaa cgtaacgcta agccgggtgc
     9601 tggaatggcc ggtcgttgta tcaccagaag gcactgcgcc acgccagcat tcagatgcat
     9661 tagtgcagag ccagggctgt aaaattcctt cgggttgtat cgaaacgctg tctgcttcgc
     9721 tatctcgtca acttacggtt gaatacgact acgtgtggtt tgtcccttct ggcgcggtaa
     9781 aagacgacct gcgtcatgcc acgctggtgg ccctgcctgt tccgggacat ggtgcaggcg
     9841 aaccgattgg aatactgacc cgcgtagatg cgacgttctc ttctggttgc cagttgatga
     9901 ttaacgctat tcgaaaatca atgccgttct gaaaggtgaa gggatctgtc gatccctcct
     9961 tgaacatttt cacaccgtag cgaaactaac tggttcaccc gctccgcgag gttctgccga
    10021 cacagaatgt ttgttcagac ggaatacgtc caccgcctca gttaatcgtg ccgcctgttc
    10081 ttcaagggac accgccgctg ctgaggcctc ttctaccaga gaagcattct gttgcgtcac
    10141 cttatccatt tcagaaatcg cctggctaac ctgagttatg cctctacttt gttcatccga
    10201 ggcggcggcg atttcctgca tgatatgtgt gacactcgcg acggcatcaa caatagtgct
    10261 catggttttc cctgccgttg ccacctcatc cgaccccagg tcaattaacc tgactgattc
    10321 actgatcaag ccttcaatct ctttcgccgc ttgggcgctg cggcttgcga gtgtccgtac
    10381 ttcgctggcg acaacggcaa atccacggcc ttgctcaccc gctcgcgcgg cttcaacggc
    10441 agcattcagt gccagaatat tcgtctggaa agcaatactg ttgatgacgg cggtgatctc
    10501 ggaaattttc ttcgaacttg tagagatagc gcccatcgtt tttactacac cggaaaccgt
    10561 ctgcccgcca tcgctggctt taatagaagc ctcttgcgcc agtttgctgg catgatgcgc
    10621 gttatccgca ttctgtttca ccgtcgcagt gagttgctcc atgctggcgg cagtttgttc
    10681 gatagccgcc gcttgttctt cggtgcgaga tgacaggtcc gcattgccag ctgaaatttc
    10741 gctggtgcca cgataaatct cttccgcgcc ctgtcgaaca gtccctactg tcatccccag
    10801 tgaatgctgc atttgctgta aatgacgact taagcgaccg atttcattac gacccgcagg
    10861 ttcatcattc atcgtcagat cgccactggc gattttttca atccgttgtg cggcatgttg
    10921 cagtggacga atgacgatcc gacgtagcac cataaatgtt atcagcgtca tgaccagggc
    10981 aagcacaaac gcgccaatca tgaacatccc acccaggcgg gtgcgctgat gggccagttc
    11041 cgccagttgg ttggctctgg tgctacgtat cttaacggct ttgttcaaaa tatcggtata
    11101 agcattatcc agcggtcgga tctgctcact ttcatgattg ataatcgctt caaacatgcc
    11161 atttttggca tatttcaaca taggttgcat acccgtgata taagcctgaa agcgttgatt
    11221 taattcagtg tcgagggctt catcagcagg tgttttcacc ggccgattct gataagcacg
    11281 ataaccttgc tgcgactgtt taatctccga ttcggcttgc gcaatattgc gcttcattgc
    11341 ttccatttcc gcaatacgac tcgcagcccc ggcctgaatc atattgatcc gggctgaacg
    11401 caaaaaatct gaactgttcg ctaacccggt ccgaatctca atctccgcag taacatcacg
    11461 ctgatcgcga tcggcctgcc agaggaaata gccagccaga gcagaactta atgcgaatag
    11521 aactaagata ccgccaagaa tgcaggcaaa taacggaacc agcctgatgt gatgcaaaaa
    11581 acctaatcga tgtgagggag ttgtattcat taccgttgtc tctcgtccag gtttactcct
    11641 gatagaaagt catcggcact tgacggtaat tacttatgac caaaaacctc tttgcgtcac
    11701 atttttacaa caattattgc agagaaaatt aatgctattg gaatgaagag agtaacgaat
    11761 ggctaaactg gctaacccga atgacgaaaa attctggaaa acagcacgca atttcgtgaa
    11821 gtgattattt tcgtccctaa ggaaacctca taggatgcct gcgggtgcct gagactttct
    11881 gttttcaggc taaccgggaa tcagaaagac gaaggccccg accgatattt ctatcaaccg
    11941 aggccaacga atgaacctag caattcagat cttagcctct tacccgccat ccggcaagga
    12001 gaaaggctat gaagcacaac cctctggtgg tgtgtctgct cattatctgc attacgattc
    12061 tgacattcac actcctgacc cgacaaacgc tctacgaact gcggttccgg gacggtgata
    12121 aggaggttgc tgcgctcatg gcctgcacgt ccaggtaagg gcaagcgcgg ggattttccc
    12181 cgcgcatctg actagcagat tcattttctc atggcacccc ttttacactt cagttagtgc
    12241 atgttttttt cgaggcacta atagaaccat cattacagac aaatttgccg cctgccgtac
    12301 agtgtgagat cccccctttc gaaccagaac agggtttgcg cccggcatcg gcataaaagg
    12361 aaacaagcgt tcccataaac aaaataagtg ccagtttttt catataacct ccatggttta
    12421 tcgaatctta tgttcgattt gaacgttata cgctggttta ttttgatacc gttgatgaga
    12481 tcaaagatcg ggtgaattga ttaaaaataa gccagatgga cgtatgggca aggattatgg
    12541 ttttatttgt catacaaata aatataatag gcgcttccga tgtagacccg tattcttcgc
    12601 ctgtaccacg ggtcggtttt agtacaggcg ttttctttaa atatccttca ggaacgttta
    12661 cgcggcatca tgcgtagaag tgtgttgtcc ttccagaaat agtggtgtgc cagtgcagcc
    12721 gcagcgtgca acccgatgac aaaatatccc agattcgcca gcgtctcatg ccacgacttt
    12781 aagctatcaa cccgttcgaa attggcctct gaagcgtaag gcatcgtcaa accaaacgca
    12841 aaccacgggt tgccccggtt atacatcatc accaaaccaa tcactggcag cgcaataaac
    12901 aggagataaa tcaccaaatg tcccaaatgc gccagtcccg tcatcatcgg ttttggctta
    12961 ggtataatcg gcggggttgg gtatttcagt cttaacaaaa gacgaaccac catcagcacg
    13021 agaattgaga taccacagga aacatgaacc atgttgatga gtggccgatc gctacgtggg
    13081 aagaaaccac gaaactccat tgcgcaatac gctgcgataa ccagcaaaaa gaccagccag
    13141 tgaatgctga tttgtaacct tgagtattta ttttccataa catttcctgc tttaacataa
    13201 ttttccgtta acataacggg cttttctcaa aatttcatta aatattgttc acccgttttc
    13261 agtacgttac gtttcactgt aagaatggag attgcccatc ccacaatcct ggtctaagcc
    13321 tggaaaggat caattttcat ccgaatgttc ctgacaggag aaaaccatga gtaaagttgg
    13381 tattaacggt tttggtcgta tcggtcgact ggtgttgcgt cgattacttg aagtcaaaag
    13441 caacatagac gttgtcgcta ttaatgatct cacttcccca aaaattctcg cctacctgct
    13501 gaaacatgat tcaaactacg gaccattccc ctggagcgtt gattttacgg aagattcact
    13561 tatcgttgat gggaaaagta tcgcggttta cgccgaaaaa gaggctaaaa atattccgtg
    13621 gaaagcgaaa ggtgcagaaa tcattgtcga atgtactggc ttttatacct ccgccgagaa
    13681 atcgcaggcg catcttgatg ctggtgcgaa gaaggtgttg atttccgccc ctgccggtga
    13741 aatgaaaact atcgtttata acgtcaatga cgacactctg gatggcaacg acaccattgt
    13801 ttccgtggcg tcatgcacca ctaactgtct tgcgccgatg gccaaagcct tgcatgacag
    13861 tttcgggata gaagtcggca cgatgacaac cattcatgcc tatactggca cccagtcact
    13921 ggtggatggc ccacgtggta aagatttacg tgcttcacgc gcagcggcag aaaatatcat
    13981 tccccacact acgggggcgg caaaagccat tggtctggtg atcccggaac tgagcggcaa
    14041 actgaaaggt catgcgcaac gcgtgccggt gaaaacaggt tcggtcactg agctggtgtc
    14101 cattctcgga aaaaaagtga ctgccgaaga ggtgaataac gcacttaaac aagcaaccac
    14161 caataacgag tcatttggtt ataccgatga agaaatagtc tcttccgata tcattggcag
    14221 ccatttcggt tcggtgtttg atgccacgca aacggaaatt accgccgtgg gcgatttaca
    14281 actggtgaaa acggtcgcct ggtacgataa cgaatatggc ttcgtcacgc agcttattcg
    14341 caccctcgaa aaattcgcta aactctgacg cgcacaggcg gaggaaaaaa cctccgcctc
    14401 ttttactcat tacgactgta aataaaccac ctgggtctgc agatattcat gcaggccatg
    14461 tttaccatct gcgccgccaa taccggattt acgccatccg gcgtggaagc cttgcatagc
    14521 ttcgaagttt tcacggttga tgtaagtttc accaaacttc agccctttaa tggctttcat
    14581 cgcgacgttc agattttggg tatagattga tgaggtcagg ccgtaatcac tgtcattagc
    14641 cattgagata gcatcttcca gcgtgtcaaa tgcgaccacc ggcagaaccg ggccaaaggt
    14701 ttcctcatgc ataatcgaca tttcctggcg aacatccagc agcaatgtcg gcggataata
    14761 atatcctttc ccgtctactg ctttgccacc caacgccact ctcgcccctt cttctactgc
    14821 gcgcgccact ttttgctcga ccctttccag cgccgcggcg ttaatcaacg gccccatcgc
    14881 aatgtcgttg cgttcagcgg ggttaccaaa ttgaaccgcc tgcatcgctt cacccagccg
    14941 attgacgaac tgatcataaa tgcctttctg tacataaaca cgttctgcac agttacacac
    15001 ttgcccacta ttaatgacgc gtgaatcaac gatggctttg actgccagtt caagatcggc
    15061 atcgtccatt acgatagctg gtgctttacc acccaattcc aggcacactt tggtgatgtt
    15121 tttcgccgca gtcgccataa tcttctcacc tgcagagacg ctgcctgtca tactgaccat
    15181 tgcgaccttt gggttacccg ccagttcttg cccaacggtt tcaccacgcc ccagtacaag
    15241 gttaaacacg ccgcgcggaa ggcctatttc atcgacgatt ttggcgaatg caatcgcatt
    15301 gtttggcgta aattcactgg gtttaatgac gatggtatta ccggtcaaaa gagcgggagc
    15361 cattttgcgg gcaatgagga agaacgggaa gttccacggc agaatgccgg tagtcacacc
    15421 aagcgcacgt ttaaacaaaa gaatattttc tcctggacga tcgctttgaa taatctcgcc
    15481 ctcgtaacgc cgtgcccact ccgccatgta atcgatatag tcggcagtaa aagccacttc
    15541 gacttcagcc agctgctgga tcttgccccc ttcttcaaca atcagcgcac tgatttcact
    15601 ggcgcgttcg cggatgccgg cggagatttt gcgcaaccaa ctggcgcgtt caatagcagg
    15661 caacgcttcc cattctggtt gtgcacgttc tgctgcatcg attgccttac gggcatcctc
    15721 ggcctgacca tcgggtatgc gggaaatgac agcctctgta gcagggttta ccacatcaat
    15781 ccatgcgtct ccacgccagg taacaaactg tccatcgata tacataggat gttgaacggg
    15841 tactgacatg ggcgactcct gtgatttata tgtttgtttt cggtgaatgc attgttaatt
    15901 aacatcaatc atcagagaga cggcatcgtg aaaggacaat tctgtgaagg gcttcataaa
    15961 aacaagtgta aaaggaataa aaggcagaaa taaaagtcag gagtcatgcc cgtttgcaaa
    16021 caagtggttc acaaacgggc aatttttaac gtaacgagcg actttccatc gcctcgatga
    16081 gcaccgcatc atgtttcagc gtttgccatg catggatgac ttctgccgga aaatcaacgt
    16141 gaacgataaa atctcgcccg cggggaccgt agccatcgct atcatcgcgt aaacgcggca
    16201 gctcgccagt gagtagtgag agataacgct caaccggcca taatccttgt aactgattaa
    16261 taaagattac actgtctgcg ttatttccta actgaggaac gaatccggga taacttaacc
    16321 agcgtggtgc atcgggattg tccccagtca tacggcggaa cgtcgccatc gtgcgccgct
    16381 gcatggtggg gtcctgaaca acgatagccg tatgaactcg ttctacggcc tgattcaata
    16441 gcgcgatgct aaagcgtgcg ttttcaccgc agtttgttga ctggtcttca atccagattt
    16501 tttcatgcgg aatgtgccag aactgatgag cgatatccgc caggatggtc gcttctgctc
    16561 tgccagtggt gcggatagtg ttgtagtgcg gatgctgtgc gatggcgcta tacaaaaaag
    16621 ttgtcgagtg accgatacca ccactaatca gtaaaggaat ttgctgatcg cgagcaatct
    16681 tacatgccgc gtcgatagtc ggcataaccg cattgcctgc aaggatcacg caatcggcct
    16741 gatacggcac ctcaccggag aaatcatcct gcgccagcca ctgtccgata acatttatgg
    16801 catctatagt tgccggcgaa agcgtcggaa acggggtaat gttcatggtt cctccttatc
    16861 gttcccttaa gaatactgcg tgagataata gtgagaagcg gagcactctg aaaggaggaa
    16921 gtcgtatgtc cttaaccgcc actccgcaag cctgacatat cttttaagga aatgctgtag
    16981 ataaacatta ttcatcttgc agcagagaat tgtatttttc tgaattcctg aatttttacc
    17041 cttaccgttt tagaattaat aaaaaacccg gcgctaaatg cttaccgggt tttttcttta
    17101 tcaggcaaat agcagggtta accgctaatc tgctccatcg cctgcaaaat acgcttatct
    17161 gaaatcggat aaggcgtacc aagttgttga gcgaagtaac taacgcgcaa ctcttctatc
    17221 atccaacgga tctctttcac gtcttcatcc tcacgacgtg cgggcggcag tttgttgatc
    17281 cattgctgcc acgcctgctg gacgttttcg actttcagca tctgagcacg gtcgcgatgt
    17341 ggatcaaccg ccagtttttc cagccgtttt tcaatcgcct gcaaatatcg cagcgtgtcg
    17401 cccagccgtt tgaagccgtt accagtgaca aaaccgcgat ataccaaccc gcccatctgc
    17461 gctttaatgt cagaaagccc cagtgccatg gtcatatcca cccgcccttt cagacgtttg
    17521 ttgatattga acaccgcagt gaggatttgc tcgacctgct tcgcaatatc caccaccgtg
    17581 tcattcagtt cggcgcgcac tttttcatgc agcgcagcaa agccttcttc cgtccagacc
    17641 gggccaccat tggcgtcgat caattgatcc acaccgcagg agatacagtc gtcgatcagc
    17701 tccagcactt tgccatacgg gttaaagtac agtcccagct tggctttgtt cggtaacttt
    17761 tcatgtaaat atttgattgg cgatggaata ttcagcagca gtagacggcg aagaccgttc
    17821 cacattgcct gcttttgctc cagcgggtta tcaaacagtt tgatcgccac actgtcgcgc
    17881 tcatccacca gcgccggcca cgccttcact ttgtagttgc cacgcttctg ttcgtagctt
    17941 tccggcagct gaccaaaact ccagatatgt aagccgctct gctcgatacc gtcatccgcc
    18001 accgcagata gcgtttcctg cactttgcct ttcagcgcat ctttcagatc ttgtagcgag
    18061 cgcccttctt ttagcttctt gtttttgtca tccaccacgc ggaaggtaat tttcaggtga
    18121 tcaggcacct gatcccagtg ccagtcttcg cggtcaacgg taacgccggt catccgccgt
    18181 aactcgcgct caaggctgtc gagcaacggt aactccagcg gtttgacgcg gcctaaaaac
    18241 gcttcggcat agtttggcgc gggtacaaaa ttacggcgta ccggtttcgg caacgattta
    18301 atcagagcaa tcaccagttc gcggcgcaga ccggggatct gccattcaaa cccgctttcc
    18361 tcaacctggt taagtaacgg cagcggaata tgtacggtca caccgtcagc atccgcgccg
    18421 ggctcaaact gatagctcaa acgcagcttg agattgccct gatgccagaa gttcgggtaa
    18481 tccagcttgc tgattttttc tgcgccctct ttgatcaaca tgcttttttc aaagttgagc
    18541 aaatcagggg tttcgcggct gacttttttc caccagctgt cgaagtgacg agcggagatt
    18601 acatcgtggc tgatgcgctg gtcgtagaac tcaaacaacg tttcgtcatc aaccagaata
    18661 tcgcggcgac gtgatttgtg ttccagctct tccacttcgg cccgcagttt caggttttca
    18721 cggaagaatg cgtgacgcgt ctgccagtca ccttccacca gcgcatggcg aataaagagt
    18781 tcacggcata acgccggatc gatctggctg tagttgacct tgcgcgcggc aacaatcggc
    18841 aaaccataaa cagtgacttt ttccgttgcc atcaccgcgc cctgcgcccg ttcccagtga
    18901 ggttcgctgt aggtgcgttt aatcaaatgc tgagcgacag gctccaccca ttccgggtcg
    18961 atacgcgcag caatgcgccc ccacaggcgg ctggtttcta ccagttccgc caccattacc
    19021 catttcggcg gttttttgaa taaaccagaa ccgggaaaga tggagaaacg cgcgttacgt
    19081 gcgccggtat attcttgttt atcggcatct ttcatgccga tatgggaaag taaacccgtc
    19141 agcaacgcaa tgtgaatttc gcgatactcc gccggttcgc tgttaaccgg aatgccaagt
    19201 tctttcacca cctggcgcaa ctgggtgtag atatcctgcc attcgcgcac gcgcagatag
    19261 ttgagataat cggtacgaca caggcgacgg aaggcgttgg aagaaagcgc cttttgctgc
    19321 tcgccaagat aattccacag attcacaaac gcgaggaagt cagactcttt gtcgtggaag
    19381 cgacgatgtt tttcgtccga tgcctgctgt ttatccatcg gacgctcgcg cggatcctga
    19441 atggaaagcg cggacgtgat aatcatcgcc tcacgcacgc agccatgttt ttgcgcttcc
    19501 agcaccatac gcgccagacg tggatcgaca ggcaactgcg agagctggcg accgagcggc
    19561 gtcagtttat aggcgctggc ctgttcatcc gtggtgatcg cgcccagttc ttcgagcaga
    19621 cgcacgccat cctggatatt gcgtttatcc ggtgcttcga caaacgggaa cgcagcgata
    19681 tcgcccagcc ccagcgcggt catctgcaaa ataaccgagg ccaggttggt acgcagaatc
    19741 tccggatcgg taaactccgg gcgcgaaagg aaatcgtctt cggaatagag acgaatacag
    19801 atcccttcgg acacacgccc acagcggcct ttacgctgat tggcagacgc ctgcgaaatg
    19861 ggttcaatcg gcaaacgctg cactttggtg cgatagctgt agcggctgat acgtgctgta
    19921 ccggggtcga tgacgtattt aatccccggt acagtcagcg aggtttccgc gacgttggtc
    19981 gccagcacaa tgcgccgtcc gctgtgcgac tggaacacgc ggttctgttc gctgttcgaa
    20041 agccgcgcat aaagcggcaa gatttcggta tggcgtaagt tcagcttgtt cagcgcatcg
    20101 gcggtatcgc gaatttcccg ctcaccactc ataaagatca gaatgtcgcc agggctttcc
    20161 tggctcagtt cgtctacggc gtcaaaaatc gcctgcaact gatcgcgctc ggtgtcatcg
    20221 gcttcttcaa caatcggtcg atagcgcact tccaccggat aggtccgacc ggagacttcg
    20281 ataatcggcg cattattaaa gtggcgcgaa aagcgttccg ggtcgatagt cgcggaagtg
    20341 ataatgattt ttaggtcagg acgccgcggc agcaactctt tcaaatagcc gagcagaaaa
    20401 tcgatattca ggctgcgttc gtgcgcttcg tcaataatga tggtgtcgta ctgcatcagc
    20461 aggcggtctt gctggatctc cgccagcagg ataccgtcgg tcatcagctt gaccatcgtg
    20521 ttatcactta cgtgatcgct gaaacgcact ttgtaaccga tgcaaccgcc tggctccgtt
    20581 ttcagctctt ccgcaatacg gttcgccacc gttcttgccg ccagacgacg cggctgggta
    20641 tggccgatca gtcctttaat cccgcgcccc agctccatac agattttcgg tagctgggtc
    20701 gttttaccgg aaccggtttc cccggcgacg atcaccacct ggtgatcacg aatcgcttcg
    20761 agaatatcct gttttttctg actaactggc aaattgtccg gataagtgat ttccggtcgt
    20821 gccgcttcac gcagcaggac tttccctgcc gcctggtcaa tctctttcgc catctcctgg
    20881 aaaatggcct gttgtgcatc aggattttta accttcttaa caccgtgcag acggcgagaa
    20941 aaacgcagtc tgtcgcgcag catcaacgaa tccagccgct gctgcaaagc agtaaaggtc
    21001 aatttttgtt gttctgtcat aacgttagtg ggcagtagtc tgcctgatta attttctttt
    21061 aaatgataga tatagagtac cacatcgcgg ctttctatgc gttgttcaat aaattcgaac
    21121 ataggcttcg atttattgcg ctatctctgt ggcaggattg tgaataaagt gtcagcaagc
    21181 aacggggcat cgcccattca aacatctata aggaaacacc atgagcaagg tattagttct
    21241 taaatccagc atcctggcag ggtactctca gtctaatcag ttgtccgatt attttgttga
    21301 acaatggcgc gaaaagcact ccgctgatga aatcaccgtt cgcgacctgg ctgcaaatcc
    21361 gattccggta ctggatggcg aactggttgg cgctctgcgt ccgagcgatg cgccgctgac
    21421 tccgcgtcag caggaagctc tggcgctttc cgatgagctg attgccgagc tgaaagccca
    21481 cgacgttatc gttattgcgg caccaatgta caacttcaac atctcgactc agttgaaaaa
    21541 ctattttgac ctggttgcac gcgcaggagt tactttccgc tataccgaga acggtccgga
    21601 aggtctggta acgggtaaaa aagccatcgt tattaccagc cgcggcggga tccacaaaga
    21661 tggaccaacg gacttggtga cgccgtatct gtccacgttc ctcggcttta tcggcattac
    21721 cgatgtgaat tttgtcttcg ccgaagggat cgcatacggt ccggaaatgg cagcgaaagc
    21781 gcagtctgac gcgaaagcag ccatcgacag cattgttgct gcataaccgg acacgatctt
    21841 gatgtggact gtagttcagt ccacatcaag atccacttac ctgttttccc ataatttcag
    21901 catcgctttg tgatcttcct taagcacaat ctgcgggcgt ctggctcgaa tatagctaat
    21961 cgcttcatta acggttttac agtgtccgta acataacaac catgccgcca ccaccagcgc
    22021 actgcgcgat aatcccaatg cacaatggac cagaacgctg ccttgctctt cgcgtaatgt
    22081 ttccagcatc gccacggcct gtcggagctc cccctcttcc ggaaccacca gatccagcat
    22141 cggtacacag aaatagagtc gatcttttgt cgctcgtccc cgagggaatt caaaggtgac
    22201 gtccagaacc gcattctgtg ccggaatatg tcgtggaaac gcccctaaat aaacaccagc
    22261 agtaattttg ctcaccggct ccaggcgacg acaaaaccaa cgcatagaca gccacatccc
    22321 gatgcgccag ggcaatgtca gccagtaaac ggcgggcggg agtttcccct gactatcttt
    22381 ccctgttgtt atcgcgccaa gcccaccgta cccacggcca ataatgagta gcgataatac
    22441 tggccaacat aaccagactg accaccataa ctgaatcatc accattagct ccatcaacac
    22501 aatgcacgag cccgcgccta cgacatatgg cagtgctatt ttgattcgac gttgatcagg
    22561 tttctgataa ttccaacgac gatcgacggg caccatccag tcaatcaaca tacctaccgc
    22621 cagccctgtg atgacatcaa taaaatgatg ctgccaggtc gtcagcgtcg aaatggcgat
    22681 gagtaaaaac catccgccgc agactttacg ccacctctca gccagatgct gacgaaagtg
    22741 acgccaaagt agccagcaga gaataatatg cagcgaagga gactggttat aaggcagatc
    22801 aaacagttca agttgcgaaa atagccatcc cgtcacccca ctcacttcag gacggataaa
    22861 actaaacttc agcggataga gcaaaaaacc gcagcaggcc attaccgttg ccagaataag
    22921 ccggtggaca agtcggcgct gttcgaatgt cgtgctacaa acgaacagcg aaaatccata
    22981 taaaagatcc agactccagt aaggaacaat agtccaggga agaaaaggga tcgccgtttc
    23041 ccaaccgaat acctgactgg ggatatcatg gctgttaagg tcctgaaccg cggtgaactg
    23101 attaagagat ccataggtga agaagaaaaa cggggccagc aacaataacc agccagcgcc
    23161 ttgtagcaat acgttccgac gttcagttat cacgacgcac cgccatcgaa accgtaaaaa
    23221 taccccactc atcaatccgt tgtgtgcatt tatcaaatcc ggcatcacgc acgagtgaat
    23281 ccatctcccc ttgcgaacgc acgcgcatta cccacggttt accatcttta tgactggtta
    23341 acaccccggc aatcatctcc agttgagggt gccacggctg cccggtgtag atgagaatgc
    23401 cgcccggttc gatggcattg gcaagacctg cgagtgagtt ttttacctgc tcattttcgg
    23461 gaaacagctc atacagtcca gagacaatcg ccagcgtagg ccgcggagtt aacgcgctga
    23521 gttcctccgg gttaaacgca tcgccctgtt caaaacgcac ccgcccagac attccccgtt
    23581 gggcaatcat ctcttgcccc tgtgcaacat ttaactcgct gtaatcacgt aacaaaatat
    23641 cgcttacggc aggctcgttt gccagcgcat ccagtacata gcgcccatgc cctgcggcaa
    23701 tgtcaaccac gcggacggct aaacctttgg cgtggagatc ggcaacggct tgtttaatca
    23761 gtatttgtaa atgggtttta cgctggcgaa taccgcgcca accaatactg ttcaggtaga
    23821 ttttgtcaat taagcgcccg aatgcgttac tgccttgcgg ttgattttga tagacataat
    23881 ccagggaact gcctgaatca aagccggtgc tcattccgag atgcaggccc gcagagtgcg
    23941 ccccgaacaa tttcatcgcc tttcgcataa agcgatacgc caaatcaacc ggcgataatg
    24001 gcacggatcc acctgaaagg agccgccagc gatccgctga tggtccagtg cggtcttcat
    24061 gctgataatc aaatttttgt gacttgttag catataaacg acgaataaag ctttgcattt
    24121 tttcaaatgc ctgcgccctg ttctcttcac ccaacgtgtc gtgataaaag cctggcagca
    24181 gatgcagctc tttcagaggg ctacgtaatc tctgataaaa atcaatctgc ggttggcgat
    24241 gcaccacata gtcatcgcct gatatcagaa gttgcgtggg gagcgtaatc gccgccgcat
    24301 ctctaataat acgttcagac gttttataga gatcgagcaa gatattgacg gcaatcgccc
    24361 gtgtgatcag cggatcatta ttgaaactcg ccacccgttg ccgatcgtgg gtcaaatagc
    24421 gtcctttcac ataggaatta ataaaaaaca gaccacgcag acgatgccat aacgccagcg
    24481 caggacgtgc cagcggcata tacaatttaa ccttaaaggc cggagaagcc agcaccagcc
    24541 cgcgaattgc aggcgcataa tcatgaaccc atgtggcaac cagcactgcg ccgacgcttt
    24601 gcgcgatcac aaccacctct tccagtccaa cctggctgtc gctggcagca aaacggacaa
    24661 attcatcgac atcctgcact gaacgcgcaa gagatgggct ataaccacgc ggccccgaag
    24721 tttttccatg cccccgggca tcccatgcat aaaaagcagt atctggcatc gccagttcat
    24781 caacgagatg ttgtagacga ccagaatgtt catgaccgca atgaaataag acgatgacct
    24841 ttttcgcccc gggctgtaaa gcgggccagt gccgataaaa caacgctgta ttatcactgg
    24901 tggtaaaaaa atgttcccca gggatgcgtg aattttccat tttctctatt ttccttcagt
    24961 aacagcagta gcgtataaaa taaaaaaata ccggagcggt gaaaattaat gagtcaatcc
    25021 tgtcaaggag tccgccatgt cctggtaata gtttcccact atctttaaca ccaatatctc
    25081 gtttgatggc tgacatcact acatcgccgc aaaaaccact aataccaatt aacaaacccg
    25141 ctaataatgc ctgtaatgta tttagcggtg tcagcaacgg tccaataatc agtgacgcaa
    25201 tcatggtggt gatgacgccc cccaccagac cttccagcgt ttttcccggg ctgactttgg
    25261 ggaccacttt tcttctgccg caggatttcc cccataaata ctgtgcaata tcgtttgact
    25321 cggttaacgc caaaagaaat aacaccagta acgccccgcc ctggatattc atggatggca
    25381 acattaataa ccaggcggca tgactcaagg cgaaaacggt cattatccag ccccaaaaaa
    25441 tagcgctcac ggtatgcaga aaaccggagg gttcacccac aaaaactcgc caggtggcta
    25501 atatcagaaa cccagacaaa ggtataaata acagaaacag ctcaaagcaa ttaaaaccga
    25561 taagcaagta gttaagagga ataacccaat ataaccaccg cggaaaatgt atggatataa
    25621 gcgtacaata ttctttcaat gccagaaaac ttatcagcgc aaaaaacgtc aacgtcatcc
    25681 agcgtggacc tgaaatcacc agtgaaaaac aaatgacgat gccccaccag gtacgaatac
    25741 gtaatgtaac ctcaccccct tttctctccg gtaaccgcca tagcagaaaa tggtttatca
    25801 gcgttgctaa aattaacaac gcgaaaagtg ttaccagaga tttttccagc attatctttc
    25861 agcactcata aaaagcacac tccgacaacg attaatcgca gtccagagaa gcagaattga
    25921 agcaatgctc cataaaagat tattccattg catccactgt ggataaatgg caacggccag
    25981 cccccacaac ccaaatatta acgcacgatc gcttttgccg aatggaccgg cataactgcg
    26041 aataccgtta attgtctgcg cgagtaaacc acaaaactca gtcaatatgg tgcaaaataa
    26101 catgagtatt acgagtgatg cgttactttc cggtaaaaat aaaaacggta aatagagcgc
    26161 aatatcggaa ataacatcgc cagtttcatt caaaatcgcc cccagacgtg tttgctgatt
    26221 gcactcacgc gccaacatgc catccagcgc attgagcgcc atacggataa aaagcacgat
    26281 gggcaatagc aaaaagagga tgggttgtgc caccaacacc aacagcaatc cggtaaaaag
    26341 agaaagcgcc agtgccgtaa gagtgatgtg attcgctgta acgtggtgct tataaagcca
    26401 aaacatcgtc ggccttaaca gcgactgaaa gagcggtttt atctggtaga gcgtcattat
    26461 caatccttga taattcggtt tgcgctatgg tgcatcaatt cccctgacgg agatatcgca
    26521 acacgccgct tcattaaaaa ttcagaatat atcaaggtat tggggactca ctgccaggaa
    26581 tgattatccc caattaatca aagggttaag aaatattacg ttgtggatca atacaactca
    26641 tcaagttttc tcatcgcttg ttcccgtatt gcgcgcgtct taccgttaag cgccagctgg
    26701 cggactaaat ccagttcagg gttaacaagc tctaacgcca gcaaaggatc acgtttcagc
    26761 ctcgccttta atacatcaac tggaaaacgg ggattattca tcgccacaat ccaccactcg
    26821 ggatcaattt ctgccgccag tactgcaggc ggtgtgtggc ggttgttaag tgcgccgaac
    26881 ttgaaaaatg tgagattgct tttcgccagt gtactccacc atatacgatc cggttgtttt
    26941 gccagtacat ccagttgtgc taacgcatcg gggtcgagtg cataatagac gcgtaatgct
    27001 tcatcaacct gcgcacggga gctgttttcc tgcctcagaa aaaacttcgc ccaaatatca
    27061 ttgcgtggcg tattttgctg taattccttg aggacaacag cgggtaaatt atcccaacta
    27121 actaatgcac tctctaaaaa gtcccagtct tccaaacgta tcagggctgt ggcattttat
    27181 agcatctgat catcaacttc accgtggctg ataagctcag acaaaatcat atcagcgctg
    27241 gtttcatacg cagctgaatc tgctttcgga ttatttttcc gatgcgataa agcacgttgc
    27301 caaatttttt gtttcatcga cgtgctcaga tactgatttt tcgccagatt actccacccc
    27361 tcaggatcgt tgaaacgtgt caataaccgt tgtagagcag cgtcagagtg aattacctgc
    27421 atatcccgac agcaaaagta ggcaggggat tttttctcca tccttttaac aaaattaaac
    27481 tgtaagttat ctggcaatgc taaaaataac acacgtatta gatcatcgtc attctcgaat
    27541 ttttggtata aataggctgc aactttgtca ctctcttgtg gcgtcatccg caataattga
    27601 ccatggtgtt tcatctctgc cagcttgaac gctaagcttg tgacaacatt gtttgatgaa
    27661 tcgttcgcca actgcattgc ctgattgtag gtgagttgtc ctccagcacc gacctgacga
    27721 atagaggaat catcactgcg gatatagcca tcaataatgg attcaggcaa taaccacgca
    27781 tctttataaa gctcctgaat gatcatatcc caatctcttt tcgccagagt cattacgata
    27841 tccagaggga gattttcacg tagcgcaaga ttcaacggac cgacaccatc aggtagacga
    27901 ctcattcgcg tcattatttc gacagagagc ggttcacggt cgagcaaccc cagaacaaca
    27961 ggctggtaac ccaattgctg atcggtcaac tccagaatct cattgtcagg catatatctc
    28021 gccatacttt caatcgtttg ttcatcattg ctattaataa gttgtttgcg taggttagct
    28081 gaaattcctt taagccttgg gagagttcgg attatggcaa gatcaaatgt cgcaatatct
    28141 cgtccttcaa atgcagcaac gttgccttca tccagcattt gcaattcctg cactaactca
    28201 ggagaaggct tagggcgaaa cagcgcttct tttttcatct gattacagac attccctgcc
    28261 atgcgaatat tgcttcgctt aaaaatttct ttcagagcat cttcatcatt tattagaccc
    28321 caataatatg catcaggatt tattatcatc agcgcttttt tgacattttc agtgtcattt
    28381 ttgtcactcg cacggaccat gaattcacta tttgtcgaag acgcaacaac aggtgcaatt
    28441 atgtcattga taaatatccc acgcgcatta atactcccct taatcgtaga cggattatcg
    28501 taatatgagt cgataaatac cagtccactt gcatttatcg aaccggtaat taacagtggt
    28561 gaatccgtaa ctatattcgt tgccgtcacg ttaccgagaa taacccatgg attagaaaga
    28621 gagaaagtgg aaatattacc tttgactgtc aaattaccaa gaacaatcaa tttttgatta
    28681 ctctctatat taccttctac cactaaatcg ccacgatgca ctttccagtc cgatgcagat
    28741 aaaccgaagg ggacaagatt cacgctactg ttgatagctt gttcaacttg ttgtttatct
    28801 tcagcg
//
LOCUS       DAZGXZ010000060        28254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000060 DAZGXZ010000000
VERSION     DAZGXZ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.060"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(53..1450)
                     /gene="ccp"
                     /locus_tag="ACHKJF_002674"
     CDS             complement(53..1450)
                     /gene="ccp"
                     /locus_tag="ACHKJF_002674"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP0950010.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1855..3504
                     /locus_tag="ACHKJF_002675"
     CDS             1855..3504
                     /locus_tag="ACHKJF_002675"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0950011.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3555..4156)
                     /locus_tag="ACHKJF_002676"
                     /pseudo
     CDS             complement(3555..4156)
                     /locus_tag="ACHKJF_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            4676..5575
                     /locus_tag="ACHKJF_002677"
     CDS             4676..5575
                     /locus_tag="ACHKJF_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0950012.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5624..6637
                     /gene="yhjD"
                     /locus_tag="ACHKJF_002678"
     CDS             5624..6637
                     /gene="yhjD"
                     /locus_tag="ACHKJF_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP0950013.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7048..8370
                     /locus_tag="ACHKJF_002679"
     CDS             7048..8370
                     /locus_tag="ACHKJF_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0950014.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8604..10664)
                     /locus_tag="ACHKJF_002680"
     CDS             complement(8604..10664)
                     /locus_tag="ACHKJF_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0950015.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKNANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10734..11501)
                     /gene="pdeH"
                     /locus_tag="ACHKJF_002681"
     CDS             complement(10734..11501)
                     /gene="pdeH"
                     /locus_tag="ACHKJF_002681"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP0950016.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11733..12662
                     /gene="kdgK"
                     /locus_tag="ACHKJF_002682"
     CDS             11733..12662
                     /gene="kdgK"
                     /locus_tag="ACHKJF_002682"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP0950017.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12758..14254)
                     /locus_tag="ACHKJF_002683"
     CDS             complement(12758..14254)
                     /locus_tag="ACHKJF_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0950018.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14475..15761)
                     /gene="dctA"
                     /locus_tag="ACHKJF_002684"
     CDS             complement(14475..15761)
                     /gene="dctA"
                     /locus_tag="ACHKJF_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP0950019.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15944..17932)
                     /gene="hmsP"
                     /locus_tag="ACHKJF_002685"
     CDS             complement(15944..17932)
                     /gene="hmsP"
                     /locus_tag="ACHKJF_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP0950020.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18014..21487)
                     /gene="bcsC"
                     /locus_tag="ACHKJF_002686"
     CDS             complement(18014..21487)
                     /gene="bcsC"
                     /locus_tag="ACHKJF_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP0950021.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQHKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21469..22575)
                     /gene="bcsZ"
                     /locus_tag="ACHKJF_002687"
     CDS             complement(21469..22575)
                     /gene="bcsZ"
                     /locus_tag="ACHKJF_002687"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP0950022.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22582..24921)
                     /gene="bcsB"
                     /locus_tag="ACHKJF_002688"
     CDS             complement(22582..24921)
                     /gene="bcsB"
                     /locus_tag="ACHKJF_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP0950023.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSCDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24932..27550)
                     /gene="bcsA"
                     /locus_tag="ACHKJF_002689"
     CDS             complement(24932..27550)
                     /gene="bcsA"
                     /locus_tag="ACHKJF_002689"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP0950024.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27547..>28254)
                     /gene="bcsQ"
                     /locus_tag="ACHKJF_002690"
     CDS             complement(27547..>28254)
                     /gene="bcsQ"
                     /locus_tag="ACHKJF_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP0950025.1"
                     /translation="TTITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWA
                     RAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGR
                     YQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRI
                     GSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLAN
                     WCLLNYSGLKTPVGSAS"
BASE COUNT         6625 a         7832 c         7156 g         6641 t
ORIGIN      
        1 tattatcgta ataaaaatcg cccaaacatt cctgttcggg cgatcaaatt aattattgtt
       61 tatcctgcat atacggcgtg tacaccccgt tcagactgtg caggaaagct acgatatcat
      121 ccacatcctc ctgcggcagc tctttgccta cctgatagcg cagcatcagt ttcaccgccc
      181 cgtccagcgt cggcacgtca ccgcgatgga agtacggtgc cgttaaagca acgttacgta
      241 aaccgggtac tttctgacgc aatttatcac gctcttcttt agtcacattc atacgaccaa
      301 tatccgccgc cgtaatttcc ccaaagttaa agtctttttt cagccccaac ggttcaaagg
      361 aacgtccgcc gagaataata ccaccatgac aagttgcaca tttattatct ttaaataatt
      421 gatagccttt tttctgttgc gccgtcagcg cattttcatc tccacgcaac catttatcaa
      481 atggggaatc cggcgtaatt aatgttttct caaattcagc aatggcatca gtaatatttt
      541 cgccactgaa accttgcgga taaacttcga ggaactgcgc tttaagctgc ggatcttttt
      601 ccagcttagc aataatttcg tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg
      661 gtccacctgc ctgatcctgc aatgttgccg cacgaccatc ccagaactgc tcaacgttaa
      721 atactgagtt aaataccgtc ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg
      781 atgtttttct gccatcgacg ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg
      841 tgctatcagc cgataaacgg ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg
      901 catcggtagg cagtttttgc gggatgggct gcaccggttc attgcgatgc tccggagcag
      961 tatcattgct ggcgtaatat tccgcgcgct gttttgcaat ccaggccagt atttccgccc
     1021 gctcttcatc actcacctta cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag
     1081 tttcatactg catcacccat tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag
     1141 ccagcagtgc cgcacgcacg gcctcgaggt taaaagattt atatccaagc ttaatgtcgt
     1201 aatccatcaa ctgtttcgcg ccaggaatat aataataggc gggtaattct gccgaaggtg
     1261 tgtggcaata gtcgcatcct ttttcgcgga gaaagcctaa aaccttatta ttttcactga
     1321 cagcagatgc ctgaacatcg gcctgtttac tgcgtttatt atcgtggtac cacacataac
     1381 cagataaccc taaatagcaa atcgcgacgc cagccaggcc gatcgcggta atacgtgaga
     1441 ccattttcat tattcttccc tcacggtggt gagttattgt gacccagaaa attttccaga
     1501 atgctgcaaa cgtctctgcg agacaaagcc atcataaaaa acagggagat acgctttttg
     1561 ataggaatca atcaattaca tgaatataaa ctatcaataa gatagcctgt gcctattacc
     1621 cagatcagga atggcatatc atgattgcgg cgagagcagg attgaatgtt ctgaaaatga
     1681 aaaacaacac gaaatccgta tttttataaa ttccatggat attagtgcgt tatatttttt
     1741 gacaggttaa taaacgtgat ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac
     1801 tacccttaaa gaaaagcccg ataattagcg acgaatttcg gaggttggat ccttatgctc
     1861 aatcagaaaa ttcaaaaccc taatccagac gaactgatga tcgaagtcga tctctgctat
     1921 gagctggacc cgtatgaatt aaaactggat gagatgatcg aggcagaacc ggaacccgag
     1981 atgattgaag ggctgcccgc ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg
     2041 ttcgagcatg ttcagtcggc gaaaattttc cccgacagta aaacctttcc cgactgcgca
     2101 cccaaaatgg acccgctgga tattttaatc cgctaccgta aagtgcgccg tcatcgtgat
     2161 tttgacttgc gcaagtttgt tgaaaatcac ttctggctgc cggaggtcta ctccagcgag
     2221 tatgtatcgg acccgcaaaa ttccctgaaa gagcatatcg accagctgtg gccggtgcta
     2281 acccgcgaac cacaggatca cattccgtgg tcttctctgc tggcgctacc gcagtcatat
     2341 attgtcccgg gcggccgttt tagcgaaacc tactattggg attcctattt caccatgctg
     2401 gggctggcgg aaagtggtcg ggaagatttg ctgaaatgca tggccgataa cttcgcctgg
     2461 atgatcgaaa actatggtca catccccaac ggcaaccgca cctattattt gagccgatcg
     2521 caaccaccgg tttttgcgct gatggtggag ttgtttgaag aagatggtgt acgcggtgcg
     2581 cgccgctatc tcgaccacct taaaatggaa tatgccttct ggatggacgg tgcagaatcg
     2641 ttgatcccta atcaggccta tcgccatgtt gtgcggatgc cggacggatc gctgctcaac
     2701 cgttattggg acgatcgcga cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc
     2761 gcgaaacatt ctggtcgccc gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc
     2821 tcaggttggg attactcttc ccgttggctg cgtgatactg gtcgtctggc gagcattcgt
     2881 accacccagt tcatccccat cgatctgaat gccttcctgt ttaaactgga gagcgccatc
     2941 gccaacatct cggcgctgaa aggcgagaaa gagacagaag cgctgttccg ccagaaggcc
     3001 agtgcccgtc gcgatgcggt aaaccgttac ctctgggatg atgaaaacgg catctaccgc
     3061 gattacgact ggcgacgcga acaactggcg ctgttttccg ctgccgccat tgtgccgctc
     3121 tatgtcggca tggcgaacca tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg
     3181 ttactgacac ctggcgggat tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa
     3241 cccaatggct gggcaccgtt acaatggatg gcaattcagg gatttaaaat gtatggcgat
     3301 gaccttctgg gtgatgaaat cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg
     3361 gaacagcaca aaatgatcga gaaataccat attgccgatg gtgttccccg cgaaggcggc
     3421 ggtggcgagt atccgttgca ggatgggttt ggctggacta acggtgtggt acgccgttta
     3481 attggtttgt acggcgaacc ataatatttt tacagccagc cgctaacttc ctgctggctg
     3541 taaaattatc ctcttcagga ggagatattt aacatcattg ccgcctgggt gcgatttttc
     3601 acttccagac ggcgatacag ggattccaga tgcgctttta ccgttccggt actgatattc
     3661 agcgctctgc tgatctcctt atttgattcg cccgccgcta acatggttaa aatctcccgc
     3721 tggcgggcgc ttaacgattt gagatcttta atgtcctttt ccggcgtcgt ccgccagtct
     3781 ccaggcagaa acatcatccc catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg
     3841 gttgaatcac gaggcacaat ggccagcaca ttaaaatgaa taacttcctg taaccaccgt
     3901 ttattgcaat ccgtcgccgt gattaacacc taatctcagg aaactgcacc acggtttttt
     3961 gcagcaacca gtagcaaaac tcaccatcct gatcgccatc gagcataact aaggcttcag
     4021 ggtaactttc cagcttttgc cataactcgt ctgcctgact ggccccctga atactcactc
     4081 ctggaatacg ctgctgtaaa ctgattttca ttccatgaat aaatattgac tgcctgtcaa
     4141 acatgactat ttgcataact gaatctccac ctgaatacgt taaaaagact taagtagtgg
     4201 aagggtatta cccgcgagaa aaaataagaa ttcgccattt ggcggtggcc attctacaga
     4261 gatgacgcgt agaaaatagt taccgatata aatagttaca gctaaacgcc tgaaattaca
     4321 tgtcgagggc actatttaaa acaattttga ggatttcctt atattggtgg ttagtacgca
     4381 tgcaattaaa aatgaaactc cgcgaccaca agcaaaaata acaaacggca atgagacaaa
     4441 aataagcaca aatagccaac acgtcctcta ttcactttaa agggaatcgc tgaaaaatac
     4501 gctctgttta gggggattca cctttctcag aaagctattc cgcccttttc ctgctgagaa
     4561 atcgccacat tcgtcatgac aacattgtga aacccggcat tagatgttag aaaaaacaat
     4621 aaacaatgcg atatgcgcgt tactgtttcg ctgtgaacaa caatcggtca aagaaatgga
     4681 taaaattcac gcaatgcagt tgttcatcaa agtcgcggag ctggaaagtt tttcccgcgc
     4741 ggcggatttc tttgctttgc caaaaggaag tgtttcgcgc cagatacagg cactggaaca
     4801 tcaacttggc acccagcttc tccagcgcac cacgcgacgg gtcaaactca cgccagaagg
     4861 catgacctat tatcaacgag caaaagatgt gttgagtaat ctcagcgaac tggacggtct
     4921 gtttcaacag gatgccacca gtatcagcgg taaattacgc atcgacatcc cgccaggaat
     4981 cgcgaaaagc ctgttactgc cgcgcctgtc ggaatttctc tatctgcatc cgggaattga
     5041 gctggaactg agtagccatg accgtccggt agatattctt catgatggtt ttgattgcgt
     5101 gatacgcact ggcgcgttac cggaagatgg cgttatcgcc cgtcccctcg gcaaactgac
     5161 catggtcaac tgtgccagtc cgcactatct gacgcgcttt ggttatccgc aaagccccga
     5221 tgatctgact tcacacgcaa tagtgcgtta tacaccgcac ctgggtgtac atccgttagg
     5281 ttttgaggtt gccagcgtta atggcgtcca gtggtttaag tctggcggca tgttgacggt
     5341 aaacagtagc gaaaactatc tcgccgccgg tcttgccggt ctggggatta ttcagatccc
     5401 gcgcattgcc gtgcgcgaag ccctgcgtgc cgggcggctt attgaagtgt tgccaggcta
     5461 ccgtgccgag ccgctctccc tttcgctggt ttacccgcag cgtcgggagc tttcccggcg
     5521 tgtaaacctg tttatgcagt ggctggctgg cgtaatgaaa gagcacctgg actgaccgac
     5581 tatacttttt aagaatgacc acgacagaag gacaaaagag cggatgacgc aggaaaacga
     5641 gatcaaacgt cccacccagg atctggagca cgagccgatt aagcagttgg ataatagcga
     5701 gaaaggcggc aaagttagcc aggcgctgga aaccgtcacc accactgccg aaaaagtcca
     5761 acgccaaccg gttattgcgc acctgattcg cgcgacagaa cgctttaacg atcggctggg
     5821 taaccagttt ggtgcggcta tcacctattt ctcattttta tcgatgatcc cgattttgat
     5881 ggtgtcgttt gccgcagggg gctttgtgct ggcctcccat ccgatgttgc tacaggatat
     5941 cttcgacaaa attctgcaaa acatcagcga tccgacgcta gccgccacgt tgaaaaacac
     6001 catcaacacc gccgttcagc agcgtacgac tgtagggctt gtcggcctgg cggtggcgct
     6061 ttattccggc atcaactgga tgggtaacct gcgtgaagcg attcgtgccc agtcgcgcga
     6121 tgtctgggaa cgctcgccgc aagatcagga gaagttctgg gtaaaatatc tgcgtgattt
     6181 tatttcgctg attggtttgt tgattgcgct gattgtgacg ctttcgatca cctcggttgc
     6241 cggttcggcg cagcaaatga ttattagcgc cctgcacctg aacagcattg agtggctgaa
     6301 accgacgtgg cggttgattg gcctggcgat ttccatcttc gccaactatc tgcttttctt
     6361 ctggatcttc tggcggttac cgcgccaccg tccgcgcaaa aaagccctga ttcgcggaac
     6421 atttctggct gctattggtt ttgaagtgat taaaatcgtg atgacctaca ccctgccatc
     6481 gctgatgaaa tccccctctg gcgcagcatt tggttccgtg ctggggctga tggcattttt
     6541 ctacttcttc gcccgtttga cgctgttttg cgcggcgtgg attgccaccg ccgaatataa
     6601 agacgacccg cgaatgccag ggaaaacgca gccttaaaat aggccggatg cggcgtaaac
     6661 gccttatccg gcatacgatt ttattcggcc tggctccccg taggccggat aagatgcgcc
     6721 agcatcgcat ccggctataa tgcgcacata acctcttgaa actcttcccc agagcctctt
     6781 cagccatcta tttgggagca aacaatttca ttccaactca taaccccagc atataaatcc
     6841 agttggtaac ttttatttaa cctgaaacca gttttatcca ctatttataa aattatgtga
     6901 agcatttcat agaagaaaaa tcactggcct aaacattatc ccctttttgc ctggtttttg
     6961 accatttccg cgatttgtta cacattgaaa tatcactttt gctgtgcgta atatggctat
     7021 tcgttagcca aaaaataaga aaagattatg caagcaacag ccacaacact cgaccacgag
     7081 caagaataca cgccgatcaa ctcgcgtaat aaagtccttg tcgcctctct cattggcacg
     7141 gccattgagt tcttcgactt ttacatttac gccactgcgg ccgttattgt gtttccgcat
     7201 atcttcttcc cgcagggcga tccaacggca gcaacgctac agtcgctcgc caccttcgcc
     7261 atcgccttcg tcgcgcgccc cattggctct gccgtttttg gtcattttgg cgatcgcgtt
     7321 gggcgtaaag cgacgctggt cgcctcgttg ctaacgatgg ggatttcgac cgtggtgatt
     7381 ggtctgctgc cgggctatgc cacgattggt attttcgccc cactgctgct ggcgctggct
     7441 cgatttggtc agggtctggg cttaggcggt gaatggggcg gcgcggcgct gctggcgact
     7501 gaaaacgccc caccgcgcaa acgtgcactg tatggctcct tcccacagct gggcgcaccg
     7561 attggcttct tctttgccaa cggcactttc ttgctgcttt cctggctact gaccgatgag
     7621 cagtttatga gctggggctg gcgtgtgcca tttatcttct cggcggtgct ggtcattatc
     7681 ggcctgtatg ttcgcgtgtc gctgcatgag tcgccggtgt ttgagaaagt cgctaaagcg
     7741 aaaaaacagg tgaagatccc gctgggtacg ctgctgacca aacatgttcg cgtaaccgta
     7801 ctgggtacgt tcatcatgct ggcaacatat acgctgtttt acatcatgac ggtctactct
     7861 atgaccttca gtaccgccgc cgcgccagtt gggcttggcc tgccacgtaa cgaagtgttg
     7921 tggatgctga tgatggcagt tattggtttt ggcgtgatgg tgccagtcgc cggattactg
     7981 gctgatgcct ttggtcgccg taaaagcatg gtaattatca ccacgctgat catcctgttc
     8041 gcgctgttcg cctttaaccc gctgctcggt tctggcaacc cgattctggt ttttgccttc
     8101 ctgctgctgg ggttaagtct gatggggctg accttcgggc cgatgggcgc gctgttacca
     8161 gagctgttcc caacagaagt gcgttacacc ggagcatcgt tctcttacaa cgtagcgtcg
     8221 attctcgggg cttccgttgc gccgtatatc gcagcctggt tgcagactaa ctacgggtta
     8281 ggtgcggtgg ggttatatct ggcggcgatg gctggcttga cgttaatcgc cctgctgctg
     8341 acccatgaga cgcgacatca gtcgttgtaa gtatcgtgtg ggcagccacc ctcaccccag
     8401 ccctctccct gaaagggaga gggagaaaag cggagcgaga tgttatatcg cgaacataac
     8461 agccctcccc cagaaagtga acggaaaagc ggtactcgtt gtttcaacac gcacaaaaca
     8521 gtcccctcgc ccctttgggg agagggttag ggtgagggga acacgctgct cccgaactca
     8581 ccagcaaaca caatccaacc tcatcacttc ttcatctgcg acaatatcgt ccgacactga
     8641 ttagcctccc cttcggaagg tgagatcagt gccagtaacg ccgccgccgg tgttaccagc
     8701 gttgccagtg ccgcagcaac agcaccacgg gcaatcaacg gcccggcttt caccccagcc
     8761 tgcggatttt taaacgtccc acgcacatac agcggcgaac gcagtgtgat aatccgaatc
     8821 cctttactct ccggatcaat agtcaaatcc agctgttccg aagcaaaact tgccgtgccg
     8881 gtaacattaa tcaacgcgtt ctcagtatcg aaagcaaaaa tctgcgggcg cgccacgccg
     8941 ttggcaatat tcagattcgc cgccgcgcag ttcacccgca cctcatcgtc accaaatatc
     9001 gcaccgacaa tgtagttgcc gacattcagc ccaacaatct ccatcaggtt gcggctcacc
     9061 agcccgtcat tcatcaacag tttcaggttg ccgttactgt tgcctaaaag tgctgccacc
     9121 gagttaccgc taccgcgtag ttccgcgtca ccgttcattt cccccagcgt cttctgcatc
     9181 agttccacat cgggcatcag ttctttcagt ttcagtcgac gagcctgaat atctgcccgc
     9241 ccctgcatcg gctttttatc gccttccaga tgaatattcg ccgcaatgct gccgcccgcc
     9301 atgccaaatt tcagcggttg caggcgcagg tcagcatttt tgaggatgat atgagtagaa
     9361 agatcgctaa tcggcaggct actgccatgc tcaatacgcc gccctttgaa gcgaacatcg
     9421 gcatccataa cgtcccattt atcggtttcg aagcggtcat aaggcagcac tttgcccgca
     9481 ggctgaacgc ttttttcgcc cttcttctgt tcagaccgtt tcgacttttc tgcccctttc
     9541 ccggagtcaa cgccaattaa cggtcccagg tccgcaagcc gcaattgccg cgactcgaca
     9601 tcaccttcca gttttggtcg tggctttccg gtggtgtaga ccagagaacc gtggatatcg
     9661 ctatcgccaa ttcgcccatt aaaaccgcga taatcaaaga ccgacgattt ttcagtgtcg
     9721 attttcgcta ccagccgacc atccgtttca aacggcgggg tatcgggcag cagaacgccc
     9781 gtcagttcat agagatcacc cagtgaatcg ccagaaaatt taagccgtaa atcgacaccg
     9841 cccatcttca ttgggtcatt cacgacgcca tcaaaagcaa cacgggtgtt accagagcgg
     9901 aaatcagcct gtaccggaaa cggcgtccct tcgccacgca gcgccagcat accgcctatt
     9961 tttcccgtac ccgtgagcgg ttcaccgtta taacgtccct gcgccttcag gccaaaaacg
    10021 taatcgccca ccttttcttt atccgcttta cctttcgatc cagtaacttc gctgaacggc
    10081 agcggcttgc ctaacggatc aacaaaaatc tccagatccg ctttgcttac tttgtcatca
    10141 atggcgatcc gcccttgatc gaaaagaata ttatccagcc gaaacgacca tgccgacggc
    10201 tttgcattcg cgtttttgtt atcatcgttg gcgagattaa acgtccagtt attgttcttt
    10261 tcggagaggc gaatcaggcg cgcgtcgggc ttttcgagct tgatccacgg cagccagacc
    10321 gttttggtca gcagcgccag cggggccagc gttgcctcta cgcgtggcaa atgcaccatc
    10381 gtgacttcgg gaatatccgg tggattgcca agaatgatgt cttccgcgtg tacatggggc
    10441 cacggcaccc agctgcgcca gcctgtttct tgcttttgcc gctcccacac cacgcccaga
    10501 tcgccacgga tagcgaacgg acgattcaac tccgcagaga ctttctggtt gatggtcggt
    10561 ttgagtcgat tccagtcaaa agttgcaatc aaaatgatcg ccacgacaat caacaacaag
    10621 aaagcccctg aaatcgcagc ggttattttg cctgccttgc tcatcgttcg cttcctcata
    10681 ttcttcctgt gccagtccta aagatagtcc agccaggcgg aaaatgaggc agcttatagc
    10741 gccagaaccg ccgtattcag cgtttctatc ggtgccgggc gtgaaagaaa ccagccttgt
    10801 gcggcgaatg cgggcgagtt ctgaacatca cgccactctt ccggcgtttc tacgccctca
    10861 acaatcaccc cgcgacaata gcgattcatt agatgtaaaa gctgagaaaa gagtgtgcgt
    10921 ccttccggcg actgacgcag catcacaaac agttctcgcg cgattttgat gtagtcataa
    10981 cgcacttcac ttagtgcaga gaaatttgcc atcccggtac caaaatcatc cagccacagc
    11041 gggccaaatt cacacatcga ggcaaaggtt gaatctttcg gcagacggat atgctccacc
    11101 agttcgaaac gcagccaggg aagacgctca atctggcgca ggatttttgg ttgctgacgc
    11161 agggcgatga gcgtagggcc atcaatattg accgatgcca gcaggccgtg ctctataaag
    11221 aagtcggctt tttgcgccag caaatcaatc tgctctttca caacctccat ccgatggctg
    11281 acggtgattt cagtaaaata gcgatccggc ggcaggcgtt gcgaagggtt caagggatgc
    11341 gtgaccaccg ttaatagctc cacggccatt aaccgtccgc atgtttgata gatcggctgc
    11401 caggtgtaag cacgctcaca ctgcaaccaa aaacgccgtt cctgcaagct ctcgatgctt
    11461 gcttcagggt tgcttattcg ctggataacc tgccttatca tcaaagatgt cctgtgttta
    11521 aaagtgatgc tgcccggact cgacaaagat tatcggcgcg caagggcaga actttaccgc
    11581 ctggccgggg taaatttcct cactccacaa cggaatcttt caggaatacg ttctggctca
    11641 aaaaaataat ggaacactgt tttaatatgg ttgaccagca aaccaccaca gcgcaaacta
    11701 acgctaattt tttacagatc aggttcacga ctatgtccaa aaagattgcc gtgattggcg
    11761 aatgcatgat tgagctttcc gagaaaggcg cggacgttaa gcgcggtttc ggcggcgata
    11821 ccctgaacac ttccgtctat atcgcccgtc aggtcgatcc tgcggcatta accgttcatt
    11881 acgtaacggc gctgggaacg gacagtttta gccagcaaat gctggacgcc tggcacggcg
    11941 agaacgttga tacttccctg acccaacgga tggaaaaccg tctgccgggc ctttactaca
    12001 ttgaaaccga cagcaccggc gagcgtacgt tctactactg gcggaacgaa gccgccgcca
    12061 aattctggct ggagagtgag cagtctgcgg cgatttgcga agagctggcg aatttcgatt
    12121 atctctacct gagcgggatt agcctggcga tcttaagccc gaccagccgc gaaaagctgc
    12181 tttccctgct gcgcgaatgc cgcgccaacg gcggaaaagt gattttcgac aataactatc
    12241 gtccgcgcct gtgggccagc aaagaagaga cacagcaggt gtaccaacaa atgctggaat
    12301 gcacggatat cgccttcctg acgctggacg atgaagacgc gctgtggggt caacagccgg
    12361 tggaagacgt cattgcgcgc acccataacg cgggcgtgaa agaagtggtg gtgaaacgcg
    12421 gggcggattc ttgcctggtg tccattgctg gcgaagggtt agtggatgtt ccggcggtga
    12481 aactgccgaa agaaaaagtg atcgatacca ccgcagctgg cgactctttc agtgccggtt
    12541 atctggcggt acgtttaacg ggcggcagtg cggaaaacgc ggcgaaacgc gggcacctga
    12601 ccgcaagtac cgttattcag tatcgcggcg cgattatccc gcgtgaggcg atgccagcgt
    12661 aaaagcagca tatgcaaaag gctggataaa gtgttacccc tctatccagc tcatccataa
    12721 atacctgatg attgtgctaa ctcatcacca tcaggcatta ctgtgcaggt ggaatatccg
    12781 tcacttcagg atgtacgtca tccgtggcga cagaggtggc ggcagcagca gtcgatgggg
    12841 ccatgatttg atcccaggcc gcctgcaacg ctttcatgtt aaattccggc tcgcctttcg
    12901 gctgcagcag tattaacgcc atatcattcg acaactgctg acgcagatcc tgatttaaca
    12961 tctccaccgt caggctgtta aggaaatcct gccgcagttt ctgatactgc tccggcgcga
    13021 tatcgacgac ctgattttgc aacgaacgca tccgctgacc catcagaata tcggtatcag
    13081 cccgcgcata ggcggcaaac agtttctgca gctccagttt cttttgcgcc actaacgcat
    13141 tgaactcttc ttccggcaga cctttatcgc gaaccttcgc cagttcacgc gccaccagat
    13201 taaggttgct gttcagcttg tcgtttggtg attcgatgtt gatggcacac tgcgcacgca
    13261 gatacagcac acggcagtca aatccaagac cgatgtcttt gctgttactg gcgcttaacg
    13321 cttgctgaac atgccagaac agcgcctcgc gggccaggtc cgcacgccag tagcgcagca
    13381 gtgcggctga ttcacgaatc ggctgccacg gcgtatccca catgatagat aaccggtcct
    13441 gacgcaccgc gtcagtcata atactcaccg cttccgcacg cagcggagaa agcgtcggca
    13501 ccggagctgg cgtttcacgt ttgcctttca gttcgccaaa cgttttgttt atttggtcga
    13561 caaccgagcg cgcatccacg tttcccacca ccagtagcgt cattgcatcc ggggtgtacc
    13621 atttctgata gaaatcttta attttttccg cttcaacggg ttgtttcagc ggatcggcag
    13681 gatcgtgacc taacaaggtt gaccctttca gacgatagcg ccaccagccc tctttagtat
    13741 cggctggcca ggttgccacc atgtcctgac tttgcagcgc gtggttgatg gtttctggtg
    13801 tgatggtcaa tttgccagtg gcatttgcca gataagagag cgcttctttc agcaaatcgt
    13861 tacggttatt gggcaaactc agattaaaca gcgtggtgtc ataagagaca attaccggcg
    13921 gcatcgggcg tttagggtcg atcccctgct gccacaatga acgcgcctgt gctgcgtcaa
    13981 ggccaccgct ttgcgttagc gcaatacgag ggatggcgtg actgtaaccg ctctgttgtg
    14041 tactttcggc gagcgaaccg gtattaacca gcaggcgaat ttcaacacga tcgctgggac
    14101 gctggggggt ggtcagcact tgccactgta aaccgttgga aagcgtcccc tgttgccatg
    14161 ctggatcagg ctggagcgca tctgcctgca catagccagc agtggccatc atcagcaaac
    14221 cgcccgctaa aagtcgaatt tttgtgccct gcatgtgaac ccctgatcaa ctatcctggt
    14281 aataaaaaaa ctgcccgttg tcggtcagtc taaatgacgt taaaaacact tcgtgttaga
    14341 ccgcaagaac atgaaaatgt cacggaagaa gtgaaataaa cctgaactca cccaggtggt
    14401 aagttcaggc acaggggtca attatgcgca aacacccgca ctcggggaag ggagtgcggg
    14461 cataagtgat gagattaaga ggataattcg tgcgttttgc catccggcgc acgattattc
    14521 agcacatcgt ccagtttttt gtggtccagt tctttcaccc acttagcaac gacaatggtc
    14581 gctacgccgt taccgaccag gttagtcagc gcacgagctt ctgacataaa gcggtcgata
    14641 ccgaggatca gcgccagacc cgctaccggc aaatggccca ccgcagagag cgtcgccgcc
    14701 agcacgataa agccactacc cgttacccct gccgcccctt tagaagaaag cagcaacacg
    14761 attaacagcg tgatttggtg gacgatatcc atctgactgt tagtggcctg ggcgataaac
    14821 accgccgcca ttgtcaggta tatcgatgtg ccatcaaggt taaacgagta gcctgtcggg
    14881 atgaccagcc ccaccaccga tttgcggcag ccgagtttct ccatcttgtc gagcatacgc
    14941 ggcagcgccg actcggaaga tgaagtcccc agtacaatca gcagttcttc acggatgtag
    15001 cggataaatt tgaagatact gaaaccagtc gctttagcga ttgaacccaa taccagcacc
    15061 acaaacagga tacaggtaat gtagaaacag ataatcagct gccccagttg caccagtgtg
    15121 ccgacgccgt atttaccgat ggtaaacgcc attgccccga acgcaccaat aggtgccaga
    15181 cgcatgatca tattgatgat gccgaagatg acctgcgaga aactttcgat gacgttaaaa
    15241 atcagttggc ctttgctgcc cagacggtgg agcgcaaaac caaacagtac ggcaaacagc
    15301 agcacctgca gaatgttacc gctggcaaat gcgccaatga cgctcgccgg gatgacatcc
    15361 ataatgaagg cgacaatgcc ctggtctttc gcctgatcgg cgtaaaccgc taccgctttc
    15421 gcatcaagcg ttgccggatc gacgttcatt ccggcaccag gctgcacgac gttaacgatg
    15481 ataagaccaa taatcagcgc gatggtactg acaatctcaa agtaaagcag tgcgactgcg
    15541 ccggtacgac cgaccgcctt catgctttcc atgcccgcaa tgcccgttac gacggtacaa
    15601 aagatgacag gagcgatgat catcttaatg agcttaacga agccgtcgcc aagcggtttc
    15661 atttgctcgc ctatttcagg atagaaatgg ccaaggagaa taccaatggc tatcgctgtc
    15721 aggacctgaa agtaaaggct tttaaacaga gaggttttca tagggtgtcc tttagtaaaa
    15781 ccacaggtct tgtaaggtta tggggtacct gcggccttaa aataacaccc agacaacatc
    15841 acagaaatgt acctggatca taattgaaac aaaaaggtta aaaagtttga gctggctcgc
    15901 acaaaccagc actttttaaa gttttgtaat cagtttgggg tagctacttt tcttccaggt
    15961 aactctcttc gaagatttca atagggagtg ggcgagcaaa aaggaagccc tgggcaatac
    16021 caacgcccgc ttttgccagc cagtcgcgtt gtgcttcagt ctccacgcct tcggcaatca
    16081 tttgtaagtt caggctctgc gccagcatga taattgcagc aatcatgctg ctatcttccg
    16141 gcaagccttc aacaaacatt ttgtcgattt tcagtacgtc gattggcaac gatttcatat
    16201 gctgcagctg acgcagccct gcgtagccca tgccgaaatc atccagcgcc acccgaactc
    16261 cggcattgcg cagcggacgg aggattgcca ccgcagcatg agggtcgtca atacgtcggc
    16321 tttctgtcac ttccagaatc agtgttcccg gctgaatgcg atagcgggtt aacagttcca
    16381 gcatatccgc caccatattc gggtgcatca gttgcagcgc agagaggttt accgacaagg
    16441 gcagcataat gccgcgctct tgccaggctg caagcaatcg acaggactct tccagcaccc
    16501 agtgaccgac ggtaaccatc agcccacagc actcaatgcg atcgattaag ccatccggca
    16561 ggtcccaact gccatccggt tgctggatac gcagtaacac ttccgcactg accagtttac
    16621 cgctggtcat ctcgacctgt ggctgtaacc aaatagcaaa ctgatgattt tccagtgcat
    16681 taaggatatc gctctcttcc gtcaaccgct tctgggcggc ttccatctgc tgcggatcaa
    16741 agaactgaat ctgattcttg cctttatggc gagcggtaaa tgccgcagaa atagcgcgac
    16801 tgtaaagctg ttcggcggtg agatcgccgt agaacatcgc cacgccaatg ctacagtgcg
    16861 gacggagttg aatacgttca atcggcaggc gctcgctcat gatagtgagc acttgctgac
    16921 ctaaggtgat tgcgtgccac ggttcctgta caccgttggc aatgacagca aagtcataac
    16981 cgctaatctg cgcgaggatc atacgtggcg acagtaccga tttgagtttt tccaccagcg
    17041 tcagcagcag aatttctcgt tgcgcctctt tcagcacgcc cgcagtatca cgcagggttt
    17101 cacaggtgat gatcatcagc gcggtggttt gtttacgcgc gacaacttgc tccagcatct
    17161 ccatcagcaa ggctttgttc ggcaaatccg acaccgggaa gcgcatcgca ttttcgttct
    17221 gttcttcata atggcgctgc agcaattgct ggttgaggtt gtaactgcgc accaacatac
    17281 cgatttcatc gtcctgatgc agacgcggta atgccagttg gtgaccaaca agctcctggg
    17341 ctgggatggc gttaagttcg cgagcaatat tgcgtaacgg atgcaaaatc aggcggttaa
    17401 tgcaccagct gatggcgacg gtcaggataa gcgacaaaag taagtaaatg gtcactaacg
    17461 ttgagagggt gctcatcacg aacttataca tacggaagga atccgcctgt agtaccagat
    17521 aggcaattgg ctgcgggttt gccggacgtt cgagcgagta aacgcccagc gagatttgaa
    17581 ccggtagctc aaacaggcga gtaaccatta ccggcaccgg gcgctctgga ataaaacttt
    17641 tacgcagcgc ctggaactgg ttaggcagca ctacatcggc acggctgacc acgcctgccg
    17701 gtttaatgct ggcaagaata gcttccgctt cgggaatatc gcctttcaaa atagctgaag
    17761 ataagggttc gcggacagag cgagcaatgc tttccagttg cgtagccgtg ttatagcgat
    17821 tctgctggac cagatggaac agcaaaacgg tgcaaaaaat aaaaacgaac accaggacaa
    17881 cggctgccac cattgccatc tgcttgattg ttaacgagcg acttacgcgc aaattgtctc
    17941 cccccggaaa tcccagattg ccgcccgagt atacccgatc gcagcggcat taagagaggc
    18001 gctatctgaa aacttaccag tcggcgtaag gtatcagcgg ctgcggcggt aaatccatgt
    18061 caccctgcca tccggcggcg gaataacgta cgtagagcag gaaatggctg ggtgcgtaat
    18121 ctttcgcctg ctggatatca attgccgtgc caacaaacca gttggaagta acacgtcgtt
    18181 caagtaatgc ccgcgccgtg tagccgaagc cctgactgct gccgccatcg ttggattgcc
    18241 gcgcagcttc ttcctgccag tcggtcggga tcagattcat cagcggataa cgcggcatgg
    18301 ttttggtgcg tgaatgcgac cacgagccag acgcccccag ctcccacgac cagttttccg
    18361 tgcgctcccg ccacatcacc ggtatggcaa acgacaggta ttcctgcgga ctgtagtaac
    18421 cgccctgacc gagtgagtag ccactcagat ctttgtcgta atgccagatc atgttgttca
    18481 ggccgattgt gacgcggcga ttgttctggt taatgacctt atagtaatag cccgtcatcc
    18541 agcgcacgcg ccagttatct tcgacatttt taccggttaa ctggtcgcca ctaagcgatg
    18601 cccagacgcc gtttgcttca cctttatcgt agctcagact tagccccaca ccgtcggcac
    18661 gtacgccacc ccattttttc ccggtattgc tcggggagtc tttttgccca ccaaaggcca
    18721 gcaaagaact ggagatgggc cgacggtggg cgttaacggt gtaacccagc ggcccgatat
    18781 catcgctgta actgatgccg ccgaccacat ccaccacgtt gaagcccatc ggcgtggtac
    18841 cgatatccca gctccagacg tcatttcgcc agccgaccgc cacgctggca ccggaatccg
    18901 actggctgcg gttgccgcta cagtcctgta atgtacaggt gccccagttg tcatcccatt
    18961 taccatcggc attagtggag aaactgccga cgttcatatt gacgaaatca ctgcgaaaga
    19021 acatccgccc gtcagaatac ggcgcatcca cctgcaacat ggtagtgtgc gctttcagat
    19081 cggagtaacc accggtgccg ctcgaacccc agtaatcgtg ctcgagggtg acgttaagat
    19141 cctgctggcg atagaggtcc gccgcatcgc tgcgcacgcc gcgtttcagc cagtcatctt
    19201 tctcgtcgtt acgggtcagt cgggtaaagg tgtcgttatc ctgcggacgc gtcgtggtca
    19261 caccggatgc gaccatggcg tctttgtagg tttccagcgc ctgcgttgga tcgcccgcct
    19321 gcgcttcaaa cttcgcaccg tcgcgcagca ccatcgcgct ttccatcgac ggtggctgag
    19381 attttgcctg cgggatcaac ttattaaacg tccgctgcgc tgctgcggta tcgccaagct
    19441 gcgcctgtgc cagcgccacg cgccgctgtg tgttcagcga ggcgttatcg gtagcgggca
    19501 gtttcgccag ctggctacgt gccgccgctt tgtcaccggc agcaatatcc acttccgtca
    19561 gaccaagaat ggcgtcggcg ttagctggct cccgcgtcag gacattctga tatgcagcac
    19621 gggcggcggt gtaatcacgt cgttgttgcg cccagtcagc cagcgtgagg tcaatacgcg
    19681 tggaaggtgg ttgctgacgc agcatcgctt ccgcttcagc ttctttgccg ctttctcgca
    19741 ggcgattagc ggtttccagc acctgatcgc tttgcagtcg attaaccagc tcctgaatat
    19801 tgctgttcca ctgcgcgcgc ggcaggctgt tgatatgcgc cagcgccgct ctgtcctggt
    19861 catgaccaga gaggtacagc ccgtaagcgt aaacctgctc cgggtcgttc ggcttatgct
    19921 gcgccagatt gcgcattaac gtatcggcct ggctgcgttg tccggcctgc cagagatcct
    19981 gcgaaagtcg gtaagtaatc catacgctgc cggggtccag cgccagtcgt tgccgctgaa
    20041 gtgctgccgc ctgcgcccat ttgccctggt tttccagtgc ctctgcctgc tgtgccagac
    20101 ggtcgttttg caggctgcgt tcgatatcat caatgctacg ccgctgactg gcagagagcg
    20161 aggcgataaa cgcttcagct ttttctggcg attgctggcg gtaaatattt gccagcccgc
    20221 gcacggcgtt agtgttgccg ctgtccatac gcaaggtctg ctgataataa cgttcggcgg
    20281 cgggataatc ttttcgcgcc atcgccacat cgcccagccc cagcactgca taactgtcgg
    20341 tgttatcgac attacgcgcc tgctggaaca ggcgttctgc ccggtcagga ttattggctt
    20401 tcagcgcagc atcgccctgc tggatcgcca gccagtagcg gtttactttc agcagactgt
    20461 tccatttgtc gttgttgctg ctgtgcgggt ccagtgcgag ggctttttcc agattcgcca
    20521 ctgcattggc gcgatcgcct ttctgagaat acgcctggcc cagcgccccc agagcttcac
    20581 tgtctttcgg gttcgcccgc accgcctgtt gtagttcggg aatggcttta cccgccatac
    20641 cagagtccac cgccgctaaa ccttgcgcac gagcgcggaa agcaggatcg gccagctgtt
    20701 tttgctgttc tgccagttgc gattgcgcag ccgccacgct atcgccatca ctaaagatcg
    20761 agagatattt tttcagcgcc gacacactgg catcactgac gggcatgtct ttaatctgcc
    20821 cgtaccagat tttagaggcc ccttcgcgcc cggcgttcga ttttgccatc tgttccagga
    20881 cggcaaaacc ttcgtcacgg cgatcgctac taaacagcaa tagcgccaga ttgttttgca
    20941 ggcccgtatt gcccggtgca tccgcattga tgcgttttaa ctgattaatc gcttcgccac
    21001 ggcgagccgg aattttcgcc accgtactcc agtactcgac agcaatgtca ccttccggcg
    21061 gcgcaccgtt gaacagtttg ttgtaactcg ccacagcttc ttctgcatga ccggtcgtcg
    21121 cctgcaatcg tgcctgttgc agtgcctgac gaccatccgg cgtggaaagt agcatcgtag
    21181 tccgcgacga tttatacgca tttgaactcg gcgctaactg cgacagccga tcgagctgtt
    21241 tttgcgcgcc atcaatatcg ccctgacgta acaaagaacg gaaacgggcg gcaacgacgt
    21301 ccgggttatt cggatcaata agttccagcc gatataacga ctgttgcacc agatcttcac
    21361 gatgggtcgc ttcgcctaac cgaacttgct ccagcaactg ttgctgagcg gttggtgctg
    21421 cctcgaccat cggcatgacg gccagaccga gggaaagcgt gaatatgttt agtgtgaatt
    21481 tgcgcattcc tggccccagt caggtaataa ctcacctttt gtcgagaagc ggaaacggtg
    21541 ttgatcccag ccttgtccaa acagggtcag cacatagttg taataggcat cgctgccggg
    21601 aaagttatcg gccacgcgct ggcgctgaac ggcctgcgca tcgcggtttt gtaaaaaggg
    21661 cagcatggcg gcagaaaaac cgaccggtcc tttaccctgc gctttccccg tagccacatc
    21721 cactttttcc ggcggataac cgtttttctc agtgaatgtc gccatcggtt taaaccggtt
    21781 gagcatccgc gctttttgcg gatcgctgtc aggcatcatg cctacccaca tgtaaacgcg
    21841 gatagcgtcg tagctgctga tcaatgtttt ttcggctttt agctgccagc ctttgtcttt
    21901 ctcatagcgc acccagtctg gcgaaaaacc tttcggggcg gtttccagca ataaacgttg
    21961 attggtttcg cgcagcgtgg tccacggcgc gccaaagcgg gtgaaatact gcgccagcgt
    22021 cggcggcagg tagctggggt taaaacgcca gctgttatcc tcagcaaaac ccactttgcc
    22081 cggtaacaac atggaaccca gcccaggcac cgtcaccact tcctcccgcg cgatacgttt
    22141 tagcaacgcg ctgccgatgt cggtataacg ctgctctttc cacaaacgcc ccgcctccag
    22201 caacgaccag gccatccaga catcaccatc ggaggccgaa ttgctgtcca gcacttccca
    22261 cttactgttc tctttcttgc cccacagcca ggcgggcaaa cgttctttta aagaaccctg
    22321 agcgagattg ttctgcgtcc agtcgagaat attatcgaaa gctacacggt cgttagccgc
    22381 cagggcaaag aacataccgt aactttgccc ttcggaggtg gtgattttgc gcgcgtcgct
    22441 ggggtcgatg acgcgccctt cctgactgat gtaatccttt ttaaactgct cccaggcagg
    22501 ccaggtacag gctgcctgaa cactaaaggc agccaacagc agcatcgtca cgattccact
    22561 acgcaacaca ttcatcttca attactcgtt atccgggtta agacgacgac gactaataat
    22621 tcgcagcaga cgccacagta cccatgccag caatatcaca ctgatagccg ccagcaccgc
    22681 cagcagaatc ggatggtttg ccagcgcata ccacacgcgc tcgaaccacg gcagatgacc
    22741 tacgtaataa acgtcgccaa cacgtaggct gttgataccg gactcgcgga tcaccgcgac
    22801 cgaaccgaac atggtggcgc gtttgccgct atcgttcacc gcatcgttaa gcatttcata
    22861 accgcgtggg ctatctgcca acagcgcaat cacgctgcgc tggtcgttat acggcgactg
    22921 gaagccaatc accgccgcca tcgcaccgga agaggtcagc gttgaccggg tttctgccgc
    22981 gcgatcgctc tcgtccggca caatgccggg gaacggggtc tggcgcatcg gtgttttcac
    23041 ccagctttcg gtcgcctgca ccaataggtc gatctgctta tcgtctttca gtttgtccgg
    23101 gataccaccg atgatcatga tgtcggcatc tttgccctga atggtgctgc catcatcggt
    23161 caccgtcaga ttaatcgccg ggaagcccgt ctgtgcgccg ataaaaccaa cagtattcag
    23221 caacgtttcc atctgtgctt cgttaggcgc tttcggcatc acggtgatgg tttgcgacag
    23281 atccgccatc cggctgaatg ggaagcccgc gttagcaaag gcgcgtagat ccggcatcgg
    23341 gatgaagtgg taatacttcg agaagtcgat ggtggagtcg tcaccaatca ccacatgatt
    23401 ctgcaccggc tggaaggtaa tacagttatc caccgaaccg cccggcatcg ggttcatata
    23461 ctcaaagtcg aagcgcagct ggttggtcgc gcccagtttc agcgccggaa tagagacatc
    23521 tgttttgcca tccagcaaac cttgtaatac cggaatccgc agcagcaggc ggttcgcctc
    23581 ctgtttgctg ctcaggttga aggattgcag gaactggtta ttcaggctga tatccatccg
    23641 cgaactgtct ttcaccggcg gcatggtgta gcggtaatta atatccatat caatgccggt
    23701 actgcgcatc aggtagagat ccggcggcag gtttagcgaa acgttaatcg ctgctggctc
    23761 aagaccgctg gattgtaact gttcttcata ggttttcagt tcgccaaatg tgaccggacg
    23821 atcggtacgt acccagttcg gcgcatcgta cggcttacgc ggtagcagcg gtttcacttc
    23881 attcactacc acgctttcac cacggaacag aatgttaccc tgagcgatac ctttcgctgc
    23941 ctgcaacagg tctttgtcgt cacgaccaaa caccaccagc agtttgacgt aaggattctg
    24001 cggatggtta atcatctcaa tcacaggggc ttttaccgcc ggatgatcgc gcaggaagtc
    24061 cggccgtttg tcgttggttg caaagacaat ggcattacga tccggcagtt ggttatagag
    24121 caccgggaag ttctgcccac gccagccaga acgcgaacca aaccacgagg cgacaatggc
    24181 agaggcttgt tgcagcccaa catccggcgc acccgcaaag accatcggca aggtgttagt
    24241 acggttatcg cgcgggtcaa agaatggcac cgggaagtgt gacaggtcat tcttcacatt
    24301 cagggtctga taggtcagat ccagtccact gctccgccca acatccagcc aaagcgtggt
    24361 gctggccggg ttttcgcaca cgtcctgata atggccgaca aactccagcc gcacacggtt
    24421 gaagtcggta ataaacagtg ggttaatggg catttgcgcc agcgtttttt tacccaactg
    24481 ttctttggtc actggcagca cgcccatcag ctcatcattg agataaacct ttaactgcga
    24541 ctggacaggc agtaacgatg gcgatggcgt gtattcgagg ttgagcatcg ccttcgtcac
    24601 cacttcatcg ctgcgcatac caaactcaat gctgccgttc gggttaatgc cacgtagcac
    24661 catgctgccc ggcggcggcg caatttgcgc aaaggtcagc ttcacatcac acgaaggacc
    24721 gttctgcgcc acgactggcg catcagcgcc ctgcacgccc ggcatcactt gccccacctg
    24781 cggattttgt tccgtctggg cggctacagc tggctcagca ttgatcagtg gttgcgttgc
    24841 tggcgtcgcc tgcgtcatga aagaggggaa cgcactcatc cccatagcca ctgcacaaat
    24901 ccagaatagt tttcttttca tcgcgttatc atcattgttg agccaaagcc tgatccgatg
    24961 gttgtgccgt ttcgctccgc tccgggcggc gcggaataaa cgatacaacc caggaaacca
    25021 gagaagtcag cacacggaat atgcccttca ccgaagaagg cgcaaactcc gccagatggc
    25081 ggtagccacg gaagccgagc ttcagaatat ccagcagact ttccagcggc ttatcttccg
    25141 ggtagctgtc ctgccagagc gcccatgtat ccgcacgggc aaacgtacac tgcacaaaat
    25201 cgatatgttg ctgggtggtg agcggcatta atttcagccc aacttcatta cccatcacgc
    25261 gcgccacctg ggtcgggaag acgtattcct gctgaccgcg tttaagcaac agattcactt
    25321 tctgcccttc cagaatctgc gcctgaccgt tgatcttgat ccccaaacca ccgtcggaga
    25381 aatcctgaac ggtacacgag aagaggtgac catcttcgcg ggcaattgcc gcgggcatcg
    25441 tcatctccac tcggtgcgat cggcgtacct gtttgctttc taccgatacc gcaactgcgc
    25501 cgccaagaac aatcaggttg tagaacaccc acaccatact gacgaccacg gtgagcatct
    25561 cggttggcgg gccatagaag tagcgccaga tgcctaccgc aacgcccacc aggttgagta
    25621 gaacaaggaa gatgtagggc cgcgagatca cccagtcgac gtactcttct tccaccagtc
    25681 cacctttggc ggtgacgtta aatttgcctt tgtgcgggtt aatcagcgcc accagcgtcg
    25741 gtggtgcgat ataccacgcc agcaccgttt cgtagatttc actccagaaa gagtggcgat
    25801 atttgccctg gatcttcgag ttggtcaggc tggcatggat catatgcggc agcacgaaca
    25861 gggcgatcat caacgctggc gcatagatga tgtaggcatg aagcagcagg aacgccagcg
    25921 gcgcagtcag gaagatcagt cgtggaatgc ccgacaagaa gtggaacatg gcgttgacgt
    25981 agcacagccg ctgggcgaat ttcagccctt taccggtaag cgggttatcg agacggaaga
    26041 tttgcaccat cccgcgtgcc cagcgaatac gctgaccgat atgcgccgac agactttcgg
    26101 tcgccagccc cgccgcctgc ggaatacgca tatatgcaga ggtgtagcca cgacggtgca
    26161 ggcgcagaga cgtgtgcgca tcttcggtta ccgtttcgac agcaataccg ccaatttcat
    26221 ccaatggttt gcgacgaatt actgcacagg aaccgcagaa gaaagtggcg tcccacatat
    26281 cgttgccatc ctgcaccaga ccatagaaca gcgtgccttc gttcggtgtt ttacgaaaac
    26341 gccccaggtt gcgttcaaac gggtccggcg agaagaagtg gtgtggagtc tgcatcatcg
    26401 ccagctgttt ctctttcagg aaccagccca tggtcatttg caggaacgat cgcgttggta
    26461 cgtggtcgca gtcgaaaatc gacacgaact cgcctttggc atatttcagc gcattgttga
    26521 tgttgcccgc tttcgcatgt tcatgagtgg tacgggcgat atatttcacc cccacgtttt
    26581 gcgcaaactg gcgaaactct tccctgccgc cgtcatcgag gatccagatg ttcagtttgt
    26641 ctttcggcca gtcgataccc agcgaggcgt aaatggtatt tttcaccacg ttgagatctt
    26701 cgttgtaagt cgggacaaag atatccaccg acggccacag cgacatatct ttcggcaatg
    26761 gcaccggctg acgattcagc ggccatacta cctggaagta gccgagcacc agcacaatcc
    26821 acgcgtacgt ttcagcgaag agcagaataa gcccgcacac caggctgacc ggatcgtccc
    26881 agttcagcgt agaggtgtaa cgccaccaga tataacggca agaaacggtc agcgacagca
    26941 caatcaacat tagcgccgag aagcgccccg gcatccgccg tacgatcagc gctacccccc
    27001 acagcagcat caggaagata aactgcgcca gcgggttaaa cggctgagta acgcagatta
    27061 acgccagaat cagcgagaag gtgacgatga taccgaggat caaccgccgc gccccggcac
    27121 tcaaatgacc gagctctttt ttctcatcca gatgctgtgt tttatggcta acgcgctcag
    27181 gcagctcgtt catccattga tggtaacgtc cacgaatatt ttgcagacct gaaaatgccc
    27241 gcctgcgcgg tttcggcgtt tctttgcgcg atgcaccgat caataaccag catgtttgaa
    27301 tgagataacg gaccgggtcc agcggacgcg gacgcgaggc gttgatatgc ggatacaggt
    27361 ttttatgttc tgcgcgaata cgctgccagc gcgggtgctc cagcggaata aaaatccagg
    27421 ccaggatcat ccagaaacag ccgagcgtcg cgctgaaagc cgacgcaccg tgacgacgat
    27481 aatcgcgata acgcccgata agccgcgcgt tgaccggcgg gataagcaac caccgggtca
    27541 ggatactcat gatgcactcc cgactggcgt tttcagcccg gagtagttca acaggcacca
    27601 gttcgccagc gtcagtatct cttcagccgc cagcgcatca ctgcgatatt cacctactgg
    27661 ttgcttagcc gccaggcatt cagccatcgc ttcatcacga tgaatgagca tcggcagtaa
    27721 tcggcgctgg ctttgcaacc aaagctggta aatatcgtcc tgaacctgac tgccaatacg
    27781 gaagtcatta atcaaaatat gtgcgccatc cggcagcgct tgctgatgca gtcggatatg
    27841 gcagttggca tccacattga cgattgccag cgagtgatcg cacaaactca gcagctggtg
    27901 ggttatctgc gaggcatcac gcggtaagtc gattaaaatc cactggtaac gcccgctggc
    27961 ttttagttgc tgtaagccgg agcaaatatc gctcagccgg gtttgccagt gctgtggatt
    28021 ttcttgttct tcaatggata actgaccaaa aggcagcaaa tcgagctgcg aggtgtagcg
    28081 caacccagcg tcacgccagt cctggccatc cagcatcgct ctggcccagc cctgacggtg
    28141 ggtaaaatca acattaaatg acaggcgcaa caagttgtcc gggcaggcat cgaccaccag
    28201 aacattttct cccaacattt gtaatgacca ggctaatgcg gcggtgatgg ttgt
//
LOCUS       DAZGXZ010000061        27739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000061 DAZGXZ010000000
VERSION     DAZGXZ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.061"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..368
                     /locus_tag="ACHKJF_002691"
     CDS             135..368
                     /locus_tag="ACHKJF_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0950026.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            468..1286
                     /locus_tag="ACHKJF_002692"
     CDS             468..1286
                     /locus_tag="ACHKJF_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0950027.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            1368..1847
                     /locus_tag="ACHKJF_002693"
     CDS             1368..1847
                     /locus_tag="ACHKJF_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0950028.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            1863..2339
                     /gene="yeeS"
                     /locus_tag="ACHKJF_002694"
     CDS             1863..2339
                     /gene="yeeS"
                     /locus_tag="ACHKJF_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0950029.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRVAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            2402..2623
                     /gene="yeeT"
                     /locus_tag="ACHKJF_002695"
     CDS             2402..2623
                     /gene="yeeT"
                     /locus_tag="ACHKJF_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0950030.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2697..3065
                     /gene="cbeA"
                     /locus_tag="ACHKJF_002696"
     CDS             2697..3065
                     /gene="cbeA"
                     /locus_tag="ACHKJF_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP0950031.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            3154..3528
                     /gene="cbtA"
                     /locus_tag="ACHKJF_002697"
     CDS             3154..3528
                     /gene="cbtA"
                     /locus_tag="ACHKJF_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFP0950032.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            3525..3719
                     /gene="yeeW"
                     /locus_tag="ACHKJF_002698"
     CDS             3525..3719
                     /gene="yeeW"
                     /locus_tag="ACHKJF_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0950033.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAENGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            3765..3845
                     /locus_tag="ACHKJF_002699"
                     /pseudo
     CDS             3765..3845
                     /locus_tag="ACHKJF_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4134..4262)
                     /locus_tag="ACHKJF_002700"
                     /pseudo
     CDS             complement(4134..4262)
                     /locus_tag="ACHKJF_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4334..4516
                     /locus_tag="ACHKJF_002701"
     CDS             4334..4516
                     /locus_tag="ACHKJF_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFP0950034.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(4617..4946)
                     /gene="tmaR"
                     /locus_tag="ACHKJF_002702"
     CDS             complement(4617..4946)
                     /gene="tmaR"
                     /locus_tag="ACHKJF_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP0950035.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5118..6176)
                     /gene="yeeA"
                     /locus_tag="ACHKJF_002703"
     CDS             complement(5118..6176)
                     /gene="yeeA"
                     /locus_tag="ACHKJF_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0950036.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(6374..6847)
                     /gene="sbmC"
                     /locus_tag="ACHKJF_002704"
     CDS             complement(6374..6847)
                     /gene="sbmC"
                     /locus_tag="ACHKJF_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP0950037.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(6966..8132)
                     /gene="dacD"
                     /locus_tag="ACHKJF_002705"
     CDS             complement(6966..8132)
                     /gene="dacD"
                     /locus_tag="ACHKJF_002705"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP0950038.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            8341..9768
                     /gene="sbcB"
                     /locus_tag="ACHKJF_002706"
     CDS             8341..9768
                     /gene="sbcB"
                     /locus_tag="ACHKJF_002706"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP0950039.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(9811..10038)
                     /gene="tsuB"
                     /locus_tag="ACHKJF_002707"
     CDS             complement(9811..10038)
                     /gene="tsuB"
                     /locus_tag="ACHKJF_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP0950040.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10052..11110)
                     /gene="tsuA"
                     /locus_tag="ACHKJF_002708"
     CDS             complement(10052..11110)
                     /gene="tsuA"
                     /locus_tag="ACHKJF_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP0950041.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(11289..12647)
                     /gene="plaP"
                     /locus_tag="ACHKJF_002709"
     CDS             complement(11289..12647)
                     /gene="plaP"
                     /locus_tag="ACHKJF_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP0950042.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(12637..12699)
                     /gene="yoeI"
                     /locus_tag="ACHKJF_002710"
     CDS             complement(12637..12699)
                     /gene="yoeI"
                     /locus_tag="ACHKJF_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP0950043.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(12914..13843)
                     /gene="yeeY"
                     /locus_tag="ACHKJF_002711"
     CDS             complement(12914..13843)
                     /gene="yeeY"
                     /locus_tag="ACHKJF_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0950044.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(13889..14713)
                     /gene="yeeZ"
                     /locus_tag="ACHKJF_002712"
     CDS             complement(13889..14713)
                     /gene="yeeZ"
                     /locus_tag="ACHKJF_002712"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0950045.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(14796..15050)
                     /gene="yoeB"
                     /locus_tag="ACHKJF_002713"
     CDS             complement(14796..15050)
                     /gene="yoeB"
                     /locus_tag="ACHKJF_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP0950046.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(15047..15298)
                     /gene="yefM"
                     /locus_tag="ACHKJF_002714"
     CDS             complement(15047..15298)
                     /gene="yefM"
                     /locus_tag="ACHKJF_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP0950047.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            15582..15632
                     /gene="hisL"
                     /locus_tag="ACHKJF_002715"
     CDS             15582..15632
                     /gene="hisL"
                     /locus_tag="ACHKJF_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP0950048.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    15608..15731
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            15778..16677
                     /gene="hisG"
                     /locus_tag="ACHKJF_002716"
     CDS             15778..16677
                     /gene="hisG"
                     /locus_tag="ACHKJF_002716"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP0950049.1"
                     /translation="MIDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            16683..17987
                     /gene="hisD"
                     /locus_tag="ACHKJF_002717"
     CDS             16683..17987
                     /gene="hisD"
                     /locus_tag="ACHKJF_002717"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP0950050.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAVLPRAETARQALSASRLIVTNDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            17984..19054
                     /gene="hisC"
                     /locus_tag="ACHKJF_002718"
     CDS             17984..19054
                     /gene="hisC"
                     /locus_tag="ACHKJF_002718"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP0950051.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKVVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            19054..20121
                     /gene="hisB"
                     /locus_tag="ACHKJF_002719"
     CDS             19054..20121
                     /gene="hisB"
                     /locus_tag="ACHKJF_002719"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP0950052.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            20121..20711
                     /gene="hisH"
                     /locus_tag="ACHKJF_002720"
     CDS             20121..20711
                     /gene="hisH"
                     /locus_tag="ACHKJF_002720"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP0950053.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            20711..21448
                     /gene="hisA"
                     /locus_tag="ACHKJF_002721"
     CDS             20711..21448
                     /gene="hisA"
                     /locus_tag="ACHKJF_002721"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP0950054.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEDDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            21430..22206
                     /gene="hisF"
                     /locus_tag="ACHKJF_002722"
     CDS             21430..22206
                     /gene="hisF"
                     /locus_tag="ACHKJF_002722"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP0950055.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDETRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            22200..22811
                     /gene="hisIE"
                     /locus_tag="ACHKJF_002723"
     CDS             22200..22811
                     /gene="hisIE"
                     /locus_tag="ACHKJF_002723"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP0950056.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(22908..23885)
                     /gene="wzzB"
                     /locus_tag="ACHKJF_002724"
     CDS             complement(22908..23885)
                     /gene="wzzB"
                     /locus_tag="ACHKJF_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP0950057.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDEKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(24031..25197)
                     /gene="ugd"
                     /locus_tag="ACHKJF_002725"
     CDS             complement(24031..25197)
                     /gene="ugd"
                     /locus_tag="ACHKJF_002725"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP0950058.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPNTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPHVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(25446..26852)
                     /gene="gndA"
                     /locus_tag="ACHKJF_002726"
     CDS             complement(25446..26852)
                     /gene="gndA"
                     /locus_tag="ACHKJF_002726"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP0950059.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(26959..>27739)
                     /gene="galE"
                     /locus_tag="ACHKJF_002727"
     CDS             complement(26959..>27739)
                     /gene="galE"
                     /locus_tag="ACHKJF_002727"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0950060.1"
                     /translation="AGAKSVSESVIRPLQYYRNNVSGTFSLVEAMEESGVENLIFSSS
                     ATVYGEPKIIPVNESCAIGGTTNPYGTSKLFVENFLRDYSKASPNFKTIVLRYFNPIG
                     AHSSGKIGEDPNGIPNNLMPFICQVAIGKQKTLKIYGNDYPTKDGTGIRDYIHVMDLA
                     EGHVAALNNINQGANYRVYNLGTGIGYSVLELLEAFQKVTTRRVPYVFTKRRSGDIAE
                     CWSDPTKAYEELGWKARRGLEDMIRDAWNWQQKNPNGFKKV"
BASE COUNT         7146 a         7152 c         6762 g         6679 t
ORIGIN      
        1 atccccagga ctaccatcca ctgctgatta ccgtttccgg aaaaaacgtt aactgatccc
       61 tgaagtcagt ttcatgcttc agcctctgtc acaacgcgtg gcagaggctt tttatttatc
      121 aggagaaaac agatatgccg ggttacacag agtatgtcct ggctgaagga tctttctcat
      181 acgggcaggc ggttgctgtc ataaccgctt accgcaatgt gtttatccag gatgacccgg
      241 gaatgcattt ccgtcgggtt atccgcaacg cggaaggtca gcgacgctgg cgctgccgga
      301 attctgaacc cgatgccgga aaggtgctta atacgcggct cgccagtgac ggtcttctca
      361 gacaataaac gtcattcatt tcacccatca ggccgcgtct tctccgggag acgtggcttt
      421 ttcatttatt acaccagtaa atcttaacca ccgataagga gcaaattatg cgactagcca
      481 gtcgttttgg ttatgcgaac cagatacgcc gtgaccgtcc gctgacacac gaagaactga
      541 tgcactatgt gccggggatt ttcggggaag ataaacacac gtcccgaagc cagaactata
      601 cgtacatccc caccatcacc gtactggaaa gcctgcagcg ggaaggcttt cagccattct
      661 tcgcctgcca gacccgtgtg cgcgacccgg gccgccgggg atacacaaaa cacatgctgc
      721 gtctgcggcg ggccggagag ataaacggag aacatgtccc tgaaattatt ctgctcaact
      781 ctcatgacgg tacctccagc taccagatgc tgccggggta cttcaggttc gtctgccaga
      841 acggctgcgt ctgtggtcag tctctggggg aagtacgtgt tccgcaccgg ggaaatgtgg
      901 tggacagagt cattgaaggg gcttacgagg tggtgggcgt gtttgaccgg atagaggaga
      961 agcgtgatgc catgcagtcg ctggtcctgc cgccaccggc acgccaggcg ctggcacagg
     1021 cggcactgac ttaccgttat ggtgacgaac atcagcccgt caccaccgcc gacattctga
     1081 cgccgcgacg ccgggaggat tacggtaagg acctgtggag cgcatatcag accatccagg
     1141 agaatatgct gaaaggcggg atttccggtc gcagtgcaaa aggaaaacgt atccataccc
     1201 gggccattca cagcattgat accgacatta agctcaatcg cgcattgtgg gtgatggcag
     1261 aaacgatgct ggagagcctg cgctgatgcc gtttccctga gtgagggaat cattcagctt
     1321 tcgttcctga aaaacacaca tttatttgtt atttaaggag ttatctcatg aaaaccgttt
     1381 ctcagaatac ccccacaatt tattcagcta caacaccaga gaataatccg cctcagttgg
     1441 ttgccagcct cgtccctgat gaacagcgca tcagcttctg gccgcagcac tttggcctca
     1501 ttccacagtg ggtgaccctg gagccccgtg tcttcggctg gatggaccgt ctgtgcgaag
     1561 actactgcgg tggtatctgg aatctgtaca ccctgaacaa cggcggggca tttatggcac
     1621 ccgaaccgga tgacgatgat gacgaaacat gggtactgtt caatgccatg aacggtaacc
     1681 gcgctgaaat gagcccggaa gccgccggca ttgctgcctg tctgatgacg tacagtcatc
     1741 atgcctgtcg tacggagtgt tatgccatga cggtccatta ttaccggctg cgggattacg
     1801 ccctgcagca tccggaatgc agcgccatta tgcgcatcat cgactgaatg gagagaagta
     1861 caatgcaaca gctttccttt ctgcccggag agatgacgcc cggcgagcgc agcctcattc
     1921 aacgggccct gaaaaccctg gaccgccatc ttcatgaacc cggcgtggcc ttcacctcca
     1981 cccgtgtggc acgggaatgg ctgattctga acatggcggg actggagcgt gaagagttcc
     2041 gggtgctgta tctgaacaac cagaatcagc tgattgccgg tgaaaccctc ttcaccggca
     2101 ccatcaaccg cacggaagtc catccccggg aagtgattaa acgcgccctg taccacaatg
     2161 ccgctgccgt ggtactggca cacaatcacc cgtccggtga agtcacaccc agtaaggcag
     2221 accggcttat caccgaacgt ctggtacagg cactgggcct ggtggatatc cgggtgccgg
     2281 accatctgat agtcggtggc aaccaggttt tctcctttgc cgaacatggt ctgctttaac
     2341 ccttcacaac cacatcacac ctgttttcac ttttatcttc tgtcttcaga ggtatcccat
     2401 tatgaaaatt atcacccgtg gtgaagccat gcgtattcac caacaacatc cgacatcccg
     2461 tctttttccg ttctgtaccg gtaaataccg ctggcacggc agtgctgaag cgtataccgg
     2521 tcgtgaagtg caggatattc ccggtgtgct ggccgtgttt gctgaacgcc gtaaggacag
     2581 ttttggtccg tatgtccggc tgatgagcgt caccctgaac tgagtgggaa ttctgatgag
     2641 cagaattatc gccactaccg gaccattctt agccgatttt ctgtaaggat tttatcgtgt
     2701 cagacacact ccccgggaca acacttcccg acgacaatca cgaccgcccc tggtgggggc
     2761 tgccctgcac cgtgacgccc tgtttcgggg cacgtctggt gcaggagggt aaccggttgc
     2821 attaccttgc cgaccgcgcc ggtatcagag gcctgttcag cgatgcagat gcgtaccacc
     2881 tggaccaggc ctttccgctg ctgatgaaac aactggaact catgctcacc agcggtgaac
     2941 tgaatccccg ccatcagcat accgtcacgc tgtatgcaaa agggctgacc tgcaaagccg
     3001 ataccctcag cagttgtggt tacgtttatc tggctgttta tccgacgccc gaaatgaaaa
     3061 attaactctc cagaatagcc ttctgctacg gcctggtgtt ttcaccacgc cacttttcca
     3121 tttttatatc tgcatatcag gaaaatcttc agtatgaaaa cattacctgt attacccggg
     3181 caggcggcca gttctcgccc gtctcctgtt gaaatctggc agatactgct gtcccgactg
     3241 ctggaccagc actatggcct cacactgaat gacacacctt ttgccgatga acgtgtgatt
     3301 gagcagcata ttgaggcagg catttcactg tgtgatgcgg tgaactttct cgtggaaaaa
     3361 tacgcgctgg tgcgtaccga ccagccggga ttcagcgcct gtacccgctc tcagttaata
     3421 aacagtattg atatcctccg ggcacgccgg gcaaccggcc tgatgacccg cgacaattac
     3481 agaacggtaa ataacattac cctgggtaag tatccggagg cgaaatgatg acactggaag
     3541 ccgacagcgt taacgtacag gcgctggata tggggcacat tgtcgttgac attgatggtg
     3601 ttaatatcac tgaactgatt aataaggccg ctgaaaacgg ttattcactc cgcgtggtgg
     3661 atgaccgtga ctctaccgaa acaccggcaa cttatgccag ccctcaccag ttgctgtaag
     3721 acaatgcagt gatacagggt atagtgccat taaccacaga aacagcactg ttgtcctgta
     3781 ttaagcaggc aagagaagat gtccttaccc tgcgccatct gcagcttctg caccagaacc
     3841 ggtgaatctc actcaacagg cttcatttct cctgacgcca gcctgaatac agctggcgtt
     3901 ttcatttata aacagaaagg aaaaccgata ataatggaat ggacgccccg accatgaagc
     3961 aacggtagga tattactgat aaaccttcca gtggaggtgc gttatggccg ttttaacgat
     4021 tagcattgac ctgacaaaaa atgaattcca aatccacggt ctgggtagga acagaaaaat
     4081 ctaagctcag aaaacggatt aaagtaagcg tcaacggagc accgtattga cgcttattta
     4141 ttggtgagaa ctacgttcca tggcaggagt tcgtcaacac ggttggaggg ccattccggc
     4201 agtatgctca gaatatggcg cagatacgct tccggatcga taccgttcag acggcaggtg
     4261 cctgcttttc tggcaacaag caggaatctc agcgctgaag caggaattag aggtaaaaac
     4321 gccctaccgg gccatgaatc acccggtcat tggagtagta accaaagcag atttagccag
     4381 catggaacaa atatctttgg tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt
     4441 agtaaccagt gcagttaata acaatggagt tacagaactt tttgccttgc tgcatacaga
     4501 agaaggctgt tgttaatttc gattggttct cggatgagaa tgatgacggt gatacacaga
     4561 taagaatcaa tgagcaatga gggttgccgg gcaaccctca ttgaataaaa cgggaattac
     4621 ttcgcttcgc cgtttttcat ttcgcccata gctttcagct ttttggagat atcgcggcgt
     4681 tctttggaga gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc atagtcacct
     4741 ttcatgctgg cgatgatgcc ctggattgct tcaacgctca tccccggctt gatgtaatcg
     4801 ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc
     4861 tgaatttcac gttgcagttt gttcttacga cggaacagac gaacaaattc cagtacgtcc
     4921 tggaatgaag gcttggtagt ttccattttt atacccctga taatgtgaga gtcggattcg
     4981 tttaagcaac cgctattcgt tagggccaac attactcata gctgccgcag atgacaatgc
     5041 ttttatccct tttcactatc atacccttta tccttgctga atcgaagcag cagcaagatg
     5101 attctgaagt tcaggaatta tttgcgcaag gcccggcaaa tcagattcga tagcaactca
     5161 agctgatgcg ccgtttccat gttcagccac acataaccat agattggtgt ttccacaacc
     5221 ttcaggtcat ggtggttatt gagcaactga cgcagctctt ccacagcatc gttcaatttt
     5281 tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc
     5341 agtatttgct gcatcatgtg ctgggtatca cgaagttttt gcgcgttcaa taacacgaaa
     5401 tggctggggc gcgtggccca gtatgcattg atttgcaact ccagcataca aaccagattg
     5461 cggttaatgg tctggatacc ttcatatatc gattttggaa tacgggtttc tttgctggcg
     5521 ggcgcaatca gtccacgcat tttcacggca tcggtcagga gtttttgtag atggctttcc
     5581 agacgtgggc gttcgagtaa gttcggtgag aatgcagatt gatagacccg attatactcg
     5641 gtcagacttt tcgccagttg aatgcgccag tggatgaacg cccgttgtgg ccagataccg
     5701 gtaaacaaca ttgccagtaa agagccgagg atcacatcgc cgcttcgcca taacgccgta
     5761 tcaatttcac ctgtcgggga acccacaaca attgccagcg tcaccccaat caataaacct
     5821 tgatacggtt tcttgcccag cgccagccaa ccgcaaagga acatggccgc cgcgcaccag
     5881 actaacatca gcggtaacga gattaactcc agttgcagag cgataagacc taaaatcgaa
     5941 cccaacaccg taccgccaat acgctcaaag gcgcgaggga caacgttacc ccagaacgag
     6001 attggcccca taatcaccac catggtgacc agcggccagg tgctttccgg gatagtaaac
     6061 aggcggataa tgagaaaagt gagcaggaat gccagcgcga cccgagtacc atgcacaatg
     6121 cggtaatggc ggtatacccg gatttcaaac gggcttaatg acttatcggc acgcacgcag
     6181 aaactccgta atgagattaa aagcgctcat aactcatctg tcggccagaa ggcgcgcctc
     6241 tgaattatcc gacggacaaa ccgggaacag tgtaatttcc cggcaggagc gaaaaaattg
     6301 aaaggcgcat cttactcttt tcgctttcaa aaaaaagagt ggtcatcgcg ttaacacacc
     6361 gccctgagat gaattagtga tgttttggct gcaccgcaac atacatttcg atatcccagt
     6421 acccatcttc cgcgccattg ttcaaataaa cctcgaagca gggctttggt aacatttcat
     6481 aagcactatc ctgcaagaga ctattaaaga actgatacca gggtttagca aaatcatcac
     6541 cgactacacg agctaccgcc accgcatact gaccacctgt aatttctgtc agaatgacgc
     6601 cctcactgtt ttcgggaagc gtaaagtaac ccggcaccgt cacgacggtg tcgcagcgta
     6661 atttttcggc gggtgtttca tctggattgt cgtaatagac agcaacccac tccttcggca
     6721 caatattttt gctatctacc cacatcatca actgctcaaa gcctttcttt accgtctgtt
     6781 cccacgggcc aacgagatgg aaacctgcaa cggtacgttt ctcttcctgc ttaatctcgt
     6841 agttcatgac gcctccattg atactatttt tatatacagt atagttgcaa aattaaaacc
     6901 acaaggaatg agtgttgatt atgcgagcag actcgcactc ctgccagtct gctgcaaaag
     6961 aaaggtcagg ccttatggtg gaaataatca ctcaggcgag aaaacatgct gccttccccg
     7021 acagattcca gggtaaccag cggccagtgc gccacctgtt tatcacggtc gtaaagttca
     7081 atttccccta cccgctgatg ggcgctaatt ggcgcggtga gttctttacc atcaagggta
     7141 tatttggctt tgatatgtgg aatttcggct ttcggtagca ccatccagaa ctcttgttcc
     7201 gttcccaggt cgatattttc tttatcgcca taccagatgc gttccgttcc gacctttttc
     7261 ccacggtgca aaatttgcac cgtagtaaag ttttgttgcc cccaacgcag taattttctt
     7321 gcctcttcct cacgaccttt tgcactgtca gcccccatta ccactgcaat gagacgacgc
     7381 tgcccatcta cagccgaagc aatgaggtta aaaccggcac cagaagtatg acccgttttc
     7441 aggccgtcaa cattcatggt tttatcccac aacaacccgt tacggttttg ctgggtgata
     7501 ccgttccagg tgagactttt ctcactgtac atatgataaa actcgggctc gccgtggatg
     7561 atagcgcgag aaagcacagc taaatcataa gccgagctat gctggccagg tgcatccaga
     7621 ccatgcactg tttcaaaatg cgtatccttg agatgcagct tctcggcata gttgttcatc
     7681 atttcaacaa actgccgttg cccaccggca atatagtcag ccagagcaac acaagcgtca
     7741 tttccggaat ccacaattaa accacggctt aaatcacgta ccgatacgcg atcgccctct
     7801 ttcaaaaaca tcagtgaaga accgacaaac accggattat ctttcgccca cgcatcgcgc
     7861 cccacggtga caatatcgtc tggcgtaatg cgatgactat cgatagcgcg atccacgaca
     7921 taacccgtca tcagctttgt caggctggcg ggattgcgct gttgatgctc attacccgcg
     7981 gtgaggatct gaccggtggt gtaatccatc aatacccagg acccggcatg aatctctgga
     8041 ggctgaggtg aaaaaggaat gttttccgcc gcaaaaccag acgataagtt aaaaacgaac
     8101 aaagaagcag caataataag acggcgtttc aacagcaaac cctcaggagt ttcaaatagc
     8161 tgttcttttt acggaaatac ttatgaactg gctggaataa agtgcaagaa aatgtgacta
     8221 ccctctcatt tttatctgac atgatctgtt gccactcgct gccaaattgt ggcgctaaag
     8281 ctgattagca cggtgatatt tgatactctg gcagacagca gaaataacgg atttaaccta
     8341 atgatgaatg acggtaagca acaatctacc tttttgtttc acgattacga aacctttggc
     8401 acgcaccccg cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tagcgaattc
     8461 aatgtcatcg gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccag
     8521 cctggagcag tattaattac cggtattacc ccgcaggaag cacgggcgaa aggagaaaac
     8581 gaagccgcgt ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg
     8641 ggctacaaca atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc
     8701 tacgatcctt acgcctggag ctggcagcat gataactcgc gctgggattt gttggatgtt
     8761 atgcgtgcct gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt
     8821 ctaccgagct ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc
     8881 cacgatgcga tggctgatgt gtacgccact attgcgatgg caaagctggt aaaaacgcgt
     8941 cagccacgcc tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg
     9001 attgatgttc cgcagatgaa acccctggtg cacgtttccg gaatgtttgg agcatggcgc
     9061 ggcaatacca gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt
     9121 atggtggatt tggcaggaga catttcgcca ttactggaac tggatagcga cacattgcgc
     9181 gagcgtttat ataccgcaaa aaccgatctt ggcgataacg ccgccgttcc ggttaagctg
     9241 gtgcacatca ataaatgtcc ggtgctggcc caggcgaata cgctacgccc ggaagatgcc
     9301 gaccgactgg gaattaatcg tcagcattgc ctcgataacc tgaaaattct gcgtgaaaat
     9361 ccgcaagtgc gtgaaaaagt ggtggcgata ttcgcggaag ccgaaccgtt tacgccttca
     9421 gataacgtgg atgcacagct ttataacggc tttttcagtg acgccgatcg cgcagcaatg
     9481 aaaattgtgc tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat
     9541 aaacgaattg aaaaactgtt gttcaattat cgggcacgca acttcccggg gacgctggat
     9601 tatgccgagc agcaacgctg gctggagcac cgccgccagg tcttcacgcc cgagttttta
     9661 caaggttatg cagatgaatt gcagatgctg gcacaacaat atgccgataa caaagaaaaa
     9721 gtggcgctgt taaaagcgct ttggcagtac gcggaagaga tcgtctagtt tgaagcctcc
     9781 gccgctgata cggcggagga caatgttggg tcaggctttt tgaacggtga tgctccacgc
     9841 agcatcgcca atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccattgtgg
     9901 gattgcttct gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat
     9961 tcctgccagt gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc
    10021 cagcttttta atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg
    10081 ccttacgctg cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa
    10141 atacaaggcc aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga
    10201 tagagcaacc acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac
    10261 gtaatgttgt ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa
    10321 taaatgaccc cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt
    10381 cacccgcgac cagaaattgc agaatattgg ccgttgggga agtgattcct aacccaaaca
    10441 tgcgtccggt tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag
    10501 cagtgacaaa gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg
    10561 gcggtaaggt cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg
    10621 ttatcaccag caaaacggca accaacggcc acacagacaa attaaaagtc tcagcaatag
    10681 agttatgttc agtactgtag tgctgcaagg tttgatttaa accactggca tgtggagaac
    10741 gcatcaccgc actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct
    10801 cacccgcgcg ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccaa
    10861 agatataccc acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta
    10921 ggcccgcctg aattaacgca aaaaccccca cactttgtac cgaaattgca atcagcaagg
    10981 cgtaaaacat gcgattattt ttcgcgagat acatatcgcg aaaaccacct gtcaggcaaa
    11041 aacgcccacg ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca
    11101 ttgaaaacat aaattaatta accagatgaa tgttaatagg gaaattattc atgactggtg
    11161 gaatgcagac caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatgtc
    11221 gataaaaaag aataaaaaaa ccggagccaa atattcattc gactccggtt tattattaca
    11281 aaagatgatt acgctacgtc ttcgtactgc ggaaccgggt tacggaaact cttggtgacg
    11341 caagccaggt aaatcagacc gatagccgcc cagatcagac ccagaaccat tgagctttct
    11401 tccaggttaa cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac
    11461 tggaagtgat ctttcagcgt cttattacgc ttctcacgga tccagaactg cgagatgacc
    11521 gacaggttaa cgaaggtgaa cgccaccaac gcaccaaagt taatcagcgc tgtagccatt
    11581 accaggtcga agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc
    11641 ggagtacgcc atttcgggtg tacataaccg aagaagcttt tcgggaatac gccgtcacga
    11701 cccattacgt acatcagacg cgctacgcct gcatgcgccg ccataccgga cgccagtacg
    11761 gtaatggtgg agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg
    11821 atttcaggct gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc
    11881 aggaagtaag ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca
    11941 cgcgggatca cgcgctctgc atctttggtt tcttccgaca ggttgctgat gccgtcaaag
    12001 ccggtaaagg agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg
    12061 ccagaccaga acggacgagt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca
    12121 taaacaacca tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg
    12181 ttgaagttcg ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc
    12241 gccaccacaa acatccacga tgggatggaa ggcaccagag cttcaaaata gattttcgcc
    12301 agcagaatgt tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata
    12361 aagccgacag tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga
    12421 tagcggcgaa ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac
    12481 gcataggctg tcggcacatg accatccgtg aggcctgaaa cgataccaaa agtatcaaac
    12541 agcgtcatcg gctgcatata ggcaagaccc atcattacaa ccggaactaa cgtaagcgtt
    12601 ttacgcaatt ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt cattctcctt
    12661 tacggtgata accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac
    12721 aacaactgtc gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa
    12781 tgattggttt gaagcaaaaa ataaccgacg ctgatgaaac gtcggttttt agtcattttt
    12841 tgacagcggc gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca
    12901 taataatttt tctttaaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga
    12961 tcgcactatt tgctaaaatt tcatcccgcc accagccgct ggcaagccct gcggtctgtt
    13021 cattccagcc aatccagacg ggttcaaaga gagtttgggc gaccactttc ttctcgatta
    13081 acgcgccact atcgagaaac cgttgcgcca gataacgggg taaatagccg cagccgaggc
    13141 cgctaatttg cagctccagc ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt
    13201 cgagaagttg tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc
    13261 gatatcgctt aataatacga cggtttaacg gctcttcttc cagagctaac ggatgatgag
    13321 gcgcgaccgc aaagacttgt tccagatcgc ccaaccgtga aaaaccaaac tcactggaag
    13381 atggtggctc atgcatcgcg ccaacaataa tatccgctcg cccctgagtc agggcatccc
    13441 aggaaccacc gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa
    13501 aagcttcaat cagtggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca
    13561 gctcgttttc ccagccttca tgcagtttga tcgcctgctt ttccagttcc cgcacggtat
    13621 gcagaacttc ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt
    13681 gtccgctacg atcaagcaat tgaatattaa ggtcgctttc cagcttgtga acggtgtaac
    13741 tcagagcgga cggcgtcttg tagagtttgg ccgacgccgc cgcaaaactg ccttcttttt
    13801 ctaaggcatc gaggatcatc aacacatcca gcagtggttt catacacgcc cccttacagt
    13861 gcatgcggcg tcctgctggc gtgatcgttt actccagcgg cattaccagc ggatcgggat
    13921 actggtattc aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc
    13981 cgctgtccag actatttctg aactgcggag gttccagccc cagtaaacgg gccatctgcg
    14041 gatagaaaac attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt
    14101 taggtgcctg taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa
    14161 caccatgttc accatcaggc gcggtttttc cggcaaagaa gcgtccggga tgacgtcccg
    14221 gtccgacaag acccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc
    14281 agtcttcgag ttcttctaat actcgtccac tgttggttac cggattacgc ggggtggttt
    14341 ctttcaccgt gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg
    14401 gaatacgatg ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat
    14461 cgccggagcc gctacgacgt gccggaagcg taatgaccag tgcatcggca tccatcaggg
    14521 catccagatc gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa
    14581 tgccactcat tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc
    14641 agcctcgcgc tgaaagtgac atcgccagcg gcatgcctaa ccaccctaac ccgacaattg
    14701 cgaccttttt catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta
    14761 tcaaaactga caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca
    14821 atgagcagtg aatcgtcggt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg
    14881 gaccagaaac ctgacaaatt atgtttcagg ggttctggct tccccttacc ttcaaatggc
    14941 gttctgcggg tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc
    15001 tgccagtaca gataatcatc ccatgattcc tcagaccaga ttagtttcac tcaataatgt
    15061 ccttttctgt tcctttgcct gatttcaggc tatcgattga gtccatcaat ctccgggcgt
    15121 tagcggggga acgcagtaga taagccgtct cttccagcga gttgtattct tcgagtgaca
    15181 tcagaacaca agcctctcca ttctgacgag taataaggat cggggcatga tcttcaacgg
    15241 ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt gtacgcatgt
    15301 caatctcctc ttttgtacag ttcattgtac aatgatgggc gttaattaac tatttattaa
    15361 ttagtttgta gatcaagata ttgtcagtga gacgaaaaat ccaggcttcg ctattttgag
    15421 taccgtcagg ttagaggaca gtcctcttag ccccctcctt tccccgctca ttcattaaac
    15481 aaatccatcg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa agtggtttag
    15541 gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc gttcaattta
    15601 aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
    15661 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
    15721 ggctttttta ttgcgcggtt gataacggtt cagacaggtt taaagaggaa taacaaaatg
    15781 atagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
    15841 cgcgaattgc tggcgcgctg tggcattaaa attaatcttc acacccagcg cctgatcgcg
    15901 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat tcccggtctg
    15961 gtaatggatg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
    16021 cttaaccgcc gcgcccaggg tgaagatcca cgctacttta ccctgcgtcg tctggatttc
    16081 ggcggctgtc gtctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
    16141 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
    16201 aaaggcatct cttttaaatc ctgcttactg aacggttctg ttgaagtcgc cccgcgtgcc
    16261 ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
    16321 ctgcgcgaag tcgaagttat ctatcgctcg aaagcctgcc tgattcaacg cgatggcgaa
    16381 atggaagaat ccaaacagca actgatcgac aaactgctga cccgtattca gggtgtgatc
    16441 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgtct ggatgaagtc
    16501 atcgccctgc tgccaggtgc cgaacgccca actattctgc cgctggcggg tgaccaacag
    16561 cgcgtagcga tgcacatggt cagcagcgaa accctgttct gggaaaccat ggaaaaactg
    16621 aaagcgctgg gtgccagttc aattctggtg ctgccgattg agaagatgat ggagtgatcg
    16681 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
    16741 tgttaatgcg cccggcgatt tccgcctctg aaagcattac ccgcactgtt aacgatattc
    16801 tcgataacgt gaaagcacgc ggcgatgagg ccctgcggga atacagcgcg aagtttgata
    16861 aaaccacggt taccgcgctg aaggtgtctg cagaggagat cgccgccgcc agcgaacgcc
    16921 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa accttccaca
    16981 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagcc aggcgtgcgt tgccagcagg
    17041 tcacgcgtcc ggtagcttca gttgggttgt atattcctgg cggctccgcc ccgctcttct
    17101 caacggtatt aatgctggcg actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
    17161 gctcaccgcc gccgattgct gatgagatcc tttatgcggc gcagttgtgc ggtgtacagg
    17221 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acagaatctg
    17281 tgccgaaagt ggacaaaatc ttcgggccgg gcaacgcctt tgtcaccgaa gcgaaacgtc
    17341 aggtgagcca gcgtctggac ggtgcggcga tcgatatgcc cgccggtccg tcggaagtgc
    17401 tggtgattgc cgacagcggc gcaacgccgg atttcgtggc ctctgatttg ctctcccagg
    17461 cagaacacgg cccggactct caggtgattt tactgacgcc cgatgctgat atggcgcgtc
    17521 gtgttgccga agctgtcgaa cgccagctgg cggtactgcc gagagctgaa accgcccgtc
    17581 aggcactgag cgccagccgc ctgattgtga ccaacgattt agcgcagtgc gtggagatct
    17641 ccaaccagta cggcccggag cacctgatca tccagacccg caacgcccgc gatctggtcg
    17701 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggtg
    17761 attacgcctc cggcaccaac cacgtactgc cgacttacgg ttataccgcc acctgttcca
    17821 gcctcgggct ggcagatttc cagaagcgta tgaccgtgca ggaactgtcg aaagaaggct
    17881 tctccgcgct ggcttcaacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
    17941 aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca ccgtgactat
    18001 caccgattta gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg cgcgtcgtct
    18061 gggcggtaat ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttca
    18121 gcttactcag caaacgctca accgctaccc ggaatgccag ccgaaagtgg tgattgaaaa
    18181 ttacgcgcaa tatgcaggcg taaaaccgga gcaggtgctg gtcagccgtg gcgcggacga
    18241 aggtattgaa ctgctgattc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
    18301 cccgccaacg tacggcatgt acagcgtcag cgccgaaacg attggcgtcg agtgccgcac
    18361 agtgccgacg ctggacaact ggcaactgga cttacaaggc atttccgaca agctggatgg
    18421 cgtaaaagtg gtctatgttt gcagccccaa caacccgacc gggcaactga tcaatccgca
    18481 ggattttcgc accctgctgg agttaacccg cggtaaggcg attgtggttg ccgatgaagc
    18541 ctatatagag ttttgcccgc aggcatcgct ggctggctgg ctggcggaat atccgcacct
    18601 ggctattttg cgcacactgt cgaaagcttt tgctctggcg gggcttcgtt gcggatttac
    18661 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atcgccccct acccgctctc
    18721 gacgccggtt gccgacattg cggcccaggc gttaagcccg caggggatcg tcgccatgcg
    18781 tgagcgggtg gcgcaaatta ttgcagaacg cgaatacctg attgccgcac tgaaagagat
    18841 cccctgcgtg gagcaggttt ttgactctga aaccaactac attctggcgc gctttaaagc
    18901 ctccagcgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
    18961 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
    19021 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
    19081 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
    19141 ctcgcctttg aaccgggcgt gattccggag ctgctgaagc tgcaaaaagc gggctacaaa
    19201 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca ggcggatttt
    19261 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
    19321 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
    19381 ctggtggagc gttatctcgc tgagcaagcg atggatcgcg ctaacagtta tgtgattggc
    19441 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
    19501 cgcgaaatcc tgaactggcc gatgattggc gagcaactca ctaaacgaga ccgttacgcc
    19561 catgtagtgc gcaacaccaa agagacgcaa attgacgtcc aggtgtggct ggatcgcgaa
    19621 ggtggcagca agattaacac cggcgttggc ttctttgatc atatgctgga tcagatcgcc
    19681 acccacggcg gtttccgcat ggaaatcaac gtcaaaggcg acctctatat cgacgatcac
    19741 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc tcttggcgac
    19801 aaacgcggta tttgccgctt cggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
    19861 gcgctggata tctctggtcg cccgcacctg gaatataaag ctgaatttac ctaccagcgc
    19921 gtgggcgatc tcagcactga aatgatcgag cacttcttcc gttcgctctc atacaccatg
    19981 ggcgtgacgc tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agagagcctg
    20041 ttcaaagcct ttggtcgcac cctgcgccag gccatccgcg tggaaggcga caccctgccc
    20101 tcgtcaaaag gagtgctgta atgaacgtgg tgatccttga taccggctgc gccaatctga
    20161 actcggtaaa gtctgccatt gcgcgccacg gttatgaacc aaaagtcagc cgtgacccag
    20221 acgtcgtgtt gctggccgat aaactatttt tacccggcgt tggcaccgcg caagcagcga
    20281 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
    20341 tgggcatctg cttagggatg caactgctgg ggcggcgcag cgaagagagc aacggcgtcg
    20401 atttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccgctgc
    20461 cacatatggg ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt caggggattg
    20521 aagacggcgc gtacttttac tttgttcaca gttacgcaat gccggtcaat ccgtggacca
    20581 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
    20641 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
    20701 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
    20761 tcagggcgat tacggcaaac aacgtgatta cggtaacgac ccgctgccgc gcttacagga
    20821 ttacgccgca caaggtgccg aagtgttgca tctggtggat ctgaccgggg caaaagatcc
    20881 ggctaaacgt caaatcccgc tgattaaaac cctggtggcg ggagttaacg ttccggtgca
    20941 ggttggcggc ggcgtgcgta gcgaagatga cgtggcggcg ttactggaag cgggcgttgc
    21001 gcgtgtggtg gtcggttcca ccgcggtgaa atcaccagaa atggtgaaag gctggtttga
    21061 acgtttcggt gccgatgcct tagtgctggc gctggatgtc cgtattgacg agcaaggcaa
    21121 caagcaggtg gcagtgagcg gctggcaaga gaactcgggc gtgtcgctgg aacaactggt
    21181 ggaaacctat ctgcccgtcg gcctgaaaca tgtgctgtgt accgatattt cacgcgacgg
    21241 cacgctggca ggttctaacg tctctttata tgaagaagtg tgcgccagat atccgcaggt
    21301 ggcgtttcag tcctccggcg gtattggcga cattgatgat gttgctgccc tgcgtggcac
    21361 tggcgtgcgc ggcgtaatag ttggtcgggc attactggaa ggtaaattca ccgtgaagga
    21421 ggccatcgca tgctggcaaa acgcataatt ccatgtctcg acgttcgtga tggtcaggtg
    21481 gtaaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc gctggcaaaa
    21541 cgctacgcag aggaaggtgc agacgaactg gtgttctacg atatcaccgc ttccagcgat
    21601 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
    21661 tgtgtggcgg gtgggattaa gtctctggat gacgccgcca aaattctttc ctttggcgcg
    21721 gataaaattt ccatcaactc tcctgcgctg gcggatccga cgttaattac tcgcctggcg
    21781 gatcgctttg gcgtgcagtg tattgtggtc ggcattgata cctggtacga cgctgaaacc
    21841 ggtaaatatc atgtgaatca atataccggc gatgaaaccc gcacccgcgt cactcagtgg
    21901 gaaacgctcg actgggtaga ggaagtgcaa aaacgcggtg ccggagaaat cgtcctcaac
    21961 atgatgaatc aggacggcgt gcgtaacggt tacgacctcg aacagctgaa aaaagtgcgt
    22021 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctc
    22081 gaagccttcc gcgatgccga cgttgacggc gcgctggcgg cttccgtatt ccacaaacaa
    22141 ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
    22201 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacgga ctgatgccgg
    22261 tgattgtgca acacgcggta tccggcgaag tgttaatgct gggctatatg aacccggaag
    22321 ccttagacaa aaccatcgaa agcggcaaag tcaccttctt ctcgcgcact aaacagcgac
    22381 tgtggaccaa aggcgaaacg tcgggcaatt tcctcaacgt agtaaatatt gcgccggact
    22441 gcgacaacga cacgctactg gtactggcga atcctatcgg accaacctgc cacaaaggca
    22501 ccagcagctg cttcggcgac accgctcacc aatggctgtt cctgtatcaa ctggaacaac
    22561 tgctcgccga acgcaaatct gccgacccgg aaacctctta cactgccaaa ctgtatgcca
    22621 gcggcactaa acgcattgcg cagaaagtag gtgaagaagg ggtggaaacc gcactggcag
    22681 cgacggtaca tgaccgcttt gagctgacca acgaggcgtc tgatttgatg tatcacctgc
    22741 tggtgctgtt gcaggatcag gatctggact taacgacggt gattgagaac ctgcgtaaac
    22801 ggcatcagtg agttgcgggg taagcggatg cgatattgtt gtcgcatccg gcaaaaaaaa
    22861 cgggcaaggt gtcacccccc tgcccttttt ctttaaaact gcaaagatta cttcgcgttg
    22921 taattacgca gagcgttacg ccccaacaca atccccgcgc caaccatgcc gcccagcagc
    22981 actgccagaa tcaatgtaat tgcctttttc ggactatcgc gacgaatagg taacgtcggt
    23041 ttcattacat agcggtaagc atgaatatca agatcatcaa cctttaagtt ttcgatatcc
    23101 agcaggtttt gacgtgtctg atagtagttt ggtgagaaca ctaacggacg agtcgcctca
    23161 tgcttaatca tcgactccag agcttcgctc cccaaaagga acaacgtatc ttgcgtcaca
    23221 tcttcagtct gctgaacctg tggctttgtc acctgcgcct gattcgcata ctgcaacgct
    23281 tcctgaatct gacggatacg cagatctttc tgctcctgcg cgactacttc ctgcgttctt
    23341 aaagagtcct gcaagttttt ccgtcccaga gcaatgttgt ctttgagatc cttttctagc
    23401 tcttgattca ctttttcatc aacttgctga atgtattggg ccaacttcat ttgtgcgccc
    23461 tctgctgttt gcccaacata agaaacagtc aatggtaatt gctggttctt aacagaaggt
    23521 tcgatggtaa gtttctctgg ctcttcctga ttatccagcg tttctgctaa tgcagagaag
    23581 gcagaactga agcgaccaat taacgtctcc tgcaaatccg atactttcgg tgcagcctga
    23641 ccatagataa cattcatagc attgttatag ccagcaattt gccccacgtc gggctgagtg
    23701 ataattgctg ttgacgtcca tttctccttc gctactgcca aatatccaat agccagggca
    23761 atagccacaa tgacggaaat gatgattgtc atcttgccac gccacaactg cactagtaaa
    23821 tcaatcaaat caatctgttc cgggtcatgg ttttgcccag aaacattatt attttctact
    23881 ctcatcatta ccctaactga cggaaatttc caagtccgca tactttagcg ataatcctcg
    23941 tgaatgctat aggataagtg ataaaaataa tgaattgttg cgtaagtaac aaaagtcaat
    24001 cagggcgtaa atagccctga taacaagatg ttagtcgctg ccaaagagat cgcgggtgta
    24061 gaccttatct gccacatccc taagctcttc tgccatacgg ttggagataa tgacgtcggc
    24121 ttgttgtttg aaggtggcga gatcacgttc caggcgagag ttgaagaatg agtcttcttt
    24181 catcactggc tcgtagatga ttacttcaac acctttcgcc ttgatacgct tcataatccc
    24241 ctgaatagaa gacgcacgga agttatctga accgctcttc ataatcaggc gataaatacc
    24301 caccacatgc ggtttgcgtg acaaaatggc atcggcaata aaatctttac gcgtgcggtt
    24361 tgcatcgaca attgccgaga tcaggttatt cggcacagac tgatagttcg ccagtaactg
    24421 cttggtatct ttcggcagac aataaccacc ataaccaaac gacggattgt tgtaatggtt
    24481 gccaatacgc ggatcgagac aaacgccttc gataatctgg cgagtattca gacctaaact
    24541 ttctgcatag ctatccagtt cattaaagta cgctacgcgc atcgccagat aggtattagc
    24601 gaaaagttta atcgcttctg cttcggttga gtcggtaaac agggtcggga tattttgctt
    24661 aatcgcgcct tcctgtaaca gagcagcaaa acgttctgcg cgttctgaac gctcaccgat
    24721 gacaatacgt gaaggatgga gattatcgta aagggcttta ccctcacgga gaaattccgg
    24781 agagaaaata atattttcag tacgatattt cttatgcatc gctgcggtaa agccaacggg
    24841 taccgttgat ttgatgacca taaccgcata aggatttatc tctactacgt ccttaattac
    24901 tgattctaca ctcgatgtat tgaaataatt agtgttagga tcatagtcgg ttggagtggc
    24961 gatgatgaca taatcagcat cccggtaggc ttcattttta tctaatgtgg cattaaagtg
    25021 tattttatct gattgcaaaa actgctgaat ttccttatca acaataggag atatccgatc
    25081 attcagcata gcaacgcgtg acggtaaaat atctaatgcc acaacctcat gattttgtgc
    25141 gattagaagc ccgtttgaca agcctacata gccggttccg gaaatggtga ttttcatttc
    25201 atgctctcag aattaactta actgtgaatc atgatgtttt tagcatcctg ataagagcta
    25261 aaagttttaa cgctacgttg ttcgatggtc aacgcaacga aaaacaattc agattttctt
    25321 tataagaata gctgatattt attaagttaa tattaagcag tgaaatttag tctataagat
    25381 aaatggcaaa aaaaagcccg gtgcaatata cgccgggcct caattttact gttggttaaa
    25441 tcagattaat ccagccattc agtatggaac acaccttctt tatcaatgcg cttataagta
    25501 tgtgcaccga aatagtcacg ctgtgcctgg atcaggttcg caggcagaac agcggcacgg
    25561 tagctgtcat aataggcaac cgcagcggcg aaggtcggaa ccgggatacc gttctgtact
    25621 gcataagcaa cgacatcacg cagcgcctgc tggtaatcat cggcaatttg tttaaagtac
    25681 ggagccagca gcaggttagc gatctgcgga ttttcggcat aagcatcggt gattttctgc
    25741 aggaactgcg cacggatgat gcagccagca cggaaaatct tcgcgatttc gccgtagttc
    25801 agatcccagt tgtactcttc agacgcggcg cgtagctgag agaagccctg agcgtaagaa
    25861 acgattttgc ccagatacag cgcacggcga actttttcga tgaactcagc cttgtcgcct
    25921 gctggctgtg cttgcgggcc agagagaact ttagatgcgg caacacgctg atctttcaga
    25981 gaagagatat aacgtgcaaa cacagactcg gtaatcagcg acagcggttc gccgagatcc
    26041 agcgcactct ggctggtcca tttgcccgta cctttgttag ccgcttcatc cagaatcaca
    26101 tcaaccaggt agttaccgtc ttcatctttt ttggtgaaga tatctttggt gatgtcgatc
    26161 aggtagctgc tcagttcacc gttattccac tcggtaaagg tctgcgccaa ttcttcgttg
    26221 gtgaggttca ggccaccttt tagcagagaa taggcttcag caatcagctg catatcaccg
    26281 tattcaatac cgttgtgaac catcttcaca tagtgacctg caccatcggc accaatatag
    26341 gtaacgcatg gctcaccgtc ttcagccact gcggcaattt tggtcaggat cggcgcaacc
    26401 agttcatagg cttctttctg cccaccaggc ataatggaag gacctttcag cgcgccttct
    26461 tcaccaccgg agacaccggt accaatgaag ttaaagcctt cggcagaaag ctcacgatta
    26521 cgacgaatgg tgtcctggaa gaaggtatta ccaccatcaa tgatgatgtc gcctttatcg
    26581 aggtatggct tgagggaatc aatagcagca tccgtgcctg cacctgcttt caccattaac
    26641 aggatgcgac gaggcgtttc cagagattca acaaactctt tcaccgtata gtaaggaacc
    26701 agtttcttgc ctggattttc ggcaatcact tcttccgtct tttcacggga acggttgaaa
    26761 acagagacgg tataaccacg gctttcgatg ttaagcgcga ggttgcgtcc catcacagcc
    26821 ataccgacga cgccgatctg ttgctttgac attgtttact cctgtcatag aatatcactt
    26881 tcattacata ttaatgtaaa taatattaac tttaaattaa ttaaacaagc taacacttaa
    26941 tatatcgttt catttttatt aaactttctt aaatccattt ggattttttt gctgccaatt
    27001 ccatgcatca cgaatcatat cttctaaccc tcgccttgct ttccatccta attcttcata
    27061 cgctttggta ggatcagacc aacattcagc aatatcacct gaacgccttt tagtaaaaac
    27121 atagggaact ctccgagttg taactttctg aaatgcttct aataattcca acacagagta
    27181 tccaattcct gttccaaggt tgtaaactct ataattagct ccttgattaa tattatttaa
    27241 tgcagcaaca tgaccttcag caagatccat aacatgaata taatcgcgaa tcccagtacc
    27301 atctttagta ggataatcat ttccgtagat cttgagcgtt ttttgtttac cgatagcaac
    27361 ctggcatata aagggcatca agttattcgg aattccgtta ggatcttcac ctatttttcc
    27421 agatgagtga gcaccgatag ggttaaaata tcttaatact atagttttaa aattagggga
    27481 tgccttagaa taatcccgca aaaagttctc tacaaataac ttggacgttc catacggatt
    27541 tgtagtacca cctatagcac aactttcatt cacaggaata atctttggct caccatagac
    27601 agttgctgaa gagctaaata ttaaattttc tacaccagac tcctccattg cctcaactaa
    27661 agaaaaagtg ccagagacat tatttcgata gtattgcaaa ggtctgataa cagattcact
    27721 tacagacttt gcaccagca
//
LOCUS       DAZGXZ010000062        27513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000062 DAZGXZ010000000
VERSION     DAZGXZ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.062"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(247..993)
                     /gene="cutC"
                     /locus_tag="ACHKJF_002728"
     CDS             complement(247..993)
                     /gene="cutC"
                     /locus_tag="ACHKJF_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP0950061.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(1007..1573)
                     /gene="yecM"
                     /locus_tag="ACHKJF_002729"
     CDS             complement(1007..1573)
                     /gene="yecM"
                     /locus_tag="ACHKJF_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0950062.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGEFLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1789..3522
                     /gene="argS"
                     /locus_tag="ACHKJF_002730"
     CDS             1789..3522
                     /gene="argS"
                     /locus_tag="ACHKJF_002730"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP0950063.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3564..3608)
                     /gene="yecV"
                     /locus_tag="ACHKJF_002731"
     CDS             complement(3564..3608)
                     /gene="yecV"
                     /locus_tag="ACHKJF_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFP0950064.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3699..4187
                     /gene="yecT"
                     /locus_tag="ACHKJF_002732"
     CDS             3699..4187
                     /gene="yecT"
                     /locus_tag="ACHKJF_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP0950065.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(4307..4699)
                     /gene="flhe"
                     /locus_tag="ACHKJF_002733"
     CDS             complement(4307..4699)
                     /gene="flhe"
                     /locus_tag="ACHKJF_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP0950066.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4699..6777)
                     /gene="flhA"
                     /locus_tag="ACHKJF_002734"
     CDS             complement(4699..6777)
                     /gene="flhA"
                     /locus_tag="ACHKJF_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP0950067.1"
                     /translation="MSNLTAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6770..7918)
                     /gene="flhB"
                     /locus_tag="ACHKJF_002735"
     CDS             complement(6770..7918)
                     /gene="flhB"
                     /locus_tag="ACHKJF_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP0950068.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8120..8764)
                     /gene="cheZ"
                     /locus_tag="ACHKJF_002736"
     CDS             complement(8120..8764)
                     /gene="cheZ"
                     /locus_tag="ACHKJF_002736"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP0950069.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8775..9164)
                     /gene="cheY"
                     /locus_tag="ACHKJF_002737"
     CDS             complement(8775..9164)
                     /gene="cheY"
                     /locus_tag="ACHKJF_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP0950070.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9179..10228)
                     /gene="cheB"
                     /locus_tag="ACHKJF_002738"
     CDS             complement(9179..10228)
                     /gene="cheB"
                     /locus_tag="ACHKJF_002738"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP0950071.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10231..11091)
                     /gene="cheR"
                     /locus_tag="ACHKJF_002739"
     CDS             complement(10231..11091)
                     /gene="cheR"
                     /locus_tag="ACHKJF_002739"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP0950072.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11110..12711)
                     /gene="tap"
                     /locus_tag="ACHKJF_002740"
     CDS             complement(11110..12711)
                     /gene="tap"
                     /locus_tag="ACHKJF_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP0950073.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12757..14418)
                     /gene="tar"
                     /locus_tag="ACHKJF_002741"
     CDS             complement(12757..14418)
                     /gene="tar"
                     /locus_tag="ACHKJF_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP0950074.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14563..15066)
                     /gene="cheW"
                     /locus_tag="ACHKJF_002742"
     CDS             complement(14563..15066)
                     /gene="cheW"
                     /locus_tag="ACHKJF_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP0950075.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15087..17051)
                     /gene="cheA"
                     /locus_tag="ACHKJF_002743"
     CDS             complement(15087..17051)
                     /gene="cheA"
                     /locus_tag="ACHKJF_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP0950076.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17056..17982)
                     /gene="motB"
                     /locus_tag="ACHKJF_002744"
     CDS             complement(17056..17982)
                     /gene="motB"
                     /locus_tag="ACHKJF_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP0950077.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FKTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17979..18866)
                     /gene="motA"
                     /locus_tag="ACHKJF_002745"
     CDS             complement(17979..18866)
                     /gene="motA"
                     /locus_tag="ACHKJF_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0950078.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFVSYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18993..19571)
                     /gene="flhC"
                     /locus_tag="ACHKJF_002746"
     CDS             complement(18993..19571)
                     /gene="flhC"
                     /locus_tag="ACHKJF_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP0950079.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19574..19924)
                     /gene="flhD"
                     /locus_tag="ACHKJF_002747"
     CDS             complement(19574..19924)
                     /gene="flhD"
                     /locus_tag="ACHKJF_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP0950080.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            20704..21132
                     /gene="uspC"
                     /locus_tag="ACHKJF_002748"
     CDS             20704..21132
                     /gene="uspC"
                     /locus_tag="ACHKJF_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP0950081.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGDISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(21139..22563)
                     /gene="otsA"
                     /locus_tag="ACHKJF_002749"
     CDS             complement(21139..22563)
                     /gene="otsA"
                     /locus_tag="ACHKJF_002749"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP0950082.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(22538..23338)
                     /gene="otsB"
                     /locus_tag="ACHKJF_002750"
     CDS             complement(22538..23338)
                     /gene="otsB"
                     /locus_tag="ACHKJF_002750"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP0950083.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(23505..24491)
                     /gene="araH"
                     /locus_tag="ACHKJF_002751"
     CDS             complement(23505..24491)
                     /gene="araH"
                     /locus_tag="ACHKJF_002751"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP0950084.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(24506..26020)
                     /gene="araG"
                     /locus_tag="ACHKJF_002752"
     CDS             complement(24506..26020)
                     /gene="araG"
                     /locus_tag="ACHKJF_002752"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP0950085.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARHKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(26090..27079)
                     /gene="araF"
                     /locus_tag="ACHKJF_002753"
     CDS             complement(26090..27079)
                     /gene="araF"
                     /locus_tag="ACHKJF_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP0950086.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
BASE COUNT         6730 a         7431 c         6598 g         6754 t
ORIGIN      
        1 gctaaggaat aacccagttg ctatttagaa tatatattta tcagttttag caatttaaat
       61 cccataatca atgtgaatat aaaaaatatt attagcacaa cgctatgttc actgtcaccc
      121 gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa aaaaggcccc
      181 tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat gatgcaacgg
      241 taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa ccgccgcccc
      301 gtctacgata taacgcgaat actcgtccgc gtgttcatct gatgacatgg acaatccttg
      361 attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga cttccagcac
      421 tccggcatcg aggaagtggt gcaggttttc tgcacggact cctgctccgg ccataatgat
      481 tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca gtgcgtctga
      541 tttttgccct gatgtcagca cccgggcaat gcccaattcc gcaagattat tgagtgtata
      601 taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc
      661 agccattatt ttttccattc gtggcatatc gacattcccg tcaacatcga gaacgcccgt
      721 caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa tggcggcaaa
      781 ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat gcacagggat
      841 cgtcacccgc tggcgcacgg atttcagtac ccccaacgac ggcgttaagc ccccctcttt
      901 tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc
      961 catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttaattac gctgactgct
     1021 cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt ttgccatcgg
     1081 tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggtgaa ctggttttaa
     1141 ctgagatccc cggtaggctg agtccttcat cagaaagcaa tgccagcgca cgggcattca
     1201 gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg tgtgggtaac
     1261 gtttttcccc gggccacggt agctccacaa tagaaaactg ccagtgcgca acctgtaccg
     1321 gttcatgcaa tttaaacaga caaatcggtc tgccattgat catattttct gacaaaaact
     1381 cgccacactg ttcaaacccg cgacgccagc gttcagcagt ggcgttttga tggcaacgca
     1441 aagaaatgtg atcggcagtc agcggagtga tattcaaccc cagacggagg gaaagttcat
     1501 ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctcg tcgatagatt
     1561 gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac cctgttgccc
     1621 tgcgccaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg
     1681 ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccgccc taatttcttt
     1741 aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt gaatattcag
     1801 gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc
     1861 gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc taacggcatg
     1921 atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca ggtgctgact
     1981 catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg ctttatcaac
     2041 attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc gtccgatcgt
     2101 ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc gccaaacgtg
     2161 gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt
     2221 actctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga ctggggcact
     2281 cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc cggtgaaatg
     2341 gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga cgaagatgaa
     2401 gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga cgaatatttc
     2461 cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccagat cacctacgat
     2521 cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta caacccgatg
     2581 ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg
     2641 accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt
     2701 cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc gaaatatcgt
     2761 tatgaaacac tgcatgccga tcgcgtgcta tattacatcg actcccgtca gcatcaacac
     2821 ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg
     2881 gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa aacccgcgcg
     2941 ggtggtacag tgaaactggc cgatctgctg gatgaagccc tggaacgtgc acgccgtctg
     3001 gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc taacgcggtt
     3061 ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacgga ctacatcttc
     3121 gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca gtatgcttac
     3181 acgcgtgtat tgtccgtgtt ccgtaaggca gaaattaacg aagagcaact ggctgcagct
     3241 ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa
     3301 gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac
     3361 gatctggctg gtctgttctc tggcttctac gagcactgcc cgatcctcag cgcagaaaac
     3421 gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac gctaaagctg
     3481 ggtctggata cgctgggtat tgagacagta gagcgtatgt aatcgatttt tcgtgagagt
     3541 aaagcctgat cagggttacc tgatcaggct tttttattgc catctaaatg tattctgaaa
     3601 atggacatgc cattgttttc tgactgttgg ataagaggcc agaagcgtaa tatccggccc
     3661 cagggaaacg ataacggttg aatttaagga ataccgcagt gtttaaattt cttgtattaa
     3721 cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta aacgaccatg
     3781 acatttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact gacattaacg
     3841 tgatcaaatc atgcctgcga caagaataca atctcgtcga tgcgcaatta aataaagcct
     3901 atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgtaaaa aaacctgata
     3961 ccgaacaact taacttgctt aaaaaatcac agcgagcctg gctggatttt agggacaaag
     4021 aatgtgaatt aatcctttca aatgaggacg ttcaggattt aagtgaccct tattctgaat
     4081 cagaatggct ctcatgtatg atcatacaga ccaatacgcg aactcgccag ttgcagctat
     4141 accataactc tgaagatttt tatccaagcc ctttgacaag aggataattc acatcttttt
     4201 ggcatgtttt gttgcaagct attcctgata aataattgca acaagacatc gagccttttt
     4261 cactgagtta ttaaacatac tcgcgagcgc gtaatttttt tgtccttcag cgataattca
     4321 caatcacttc attacgttgt acctttagcg gtggaattaa ccgcccaccg cctggcactt
     4381 cccagataaa tcgcaacggt tctgctgccg ctataccgga aaaggccacg gtggttccgc
     4441 tctgcccctc taattcgaca caacgagatt gcgaacacaa gcgaacccgc agtcctgaag
     4501 gtgtcgggcc gataagctga taacgccacg ctaccagcgt catcaatcct gaagcaggtt
     4561 gtcgcgtaga aagaggcgca gacgacatcg actcaccgcg atgatttagc gtaattccta
     4621 cactacttgc ctgccacatc ccctccccgg cggcttgcac cagcagcgga aacaataata
     4681 ttgctaataa ggttctcatt atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt
     4741 atcagacagt tccagattcg acaggaccac taactgcggc aagctgcggc gcaggaagcg
     4801 agacaataat ggtcgcagcg cgtggttcac caacaatact ggcggcgcac ccagcatctc
     4861 ctgacgggat agcgcttcct gagtttgcgc cagtaaacga tccgccagcc ctggctccag
     4921 tcctcccccg ccctgcagcg cctgtagtaa caaacgttcc agcggtgtat cgaggccaat
     4981 aacatggact tcatctttgc caggaaacca ctgctgggta atcgcccgtc ccaacgccac
     5041 gcgcacgacg gcggttaatt catgtggatc gctttggatg ggcgcatgtt ccgccagcgt
     5101 ttcgagaatg gtgcgcatat cgcgaatcgg tactttttca tcgaggagat tttgcagcac
     5161 tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga tcttccgtca gctttggcat
     5221 ctcctgggcg acgcgatcca acagctgttg cgcctcctga cgaccaaaca gctctgcggc
     5281 atgctggcta atgaggtggt taagatgcgt tgctaccacc gtgctggcct caaccactgt
     5341 gtacccctga atctgcgcct gttcttttag cgcactttca atccagatag cattcaggcc
     5401 aaatgccgga tcgacggtcg cctcaccagg taacgtcccg gcagcggttc cagggttaat
     5461 cgccagccag cgccccggat aagcatcacc actgccaatc tccacgcctt tcatcaaaat
     5521 gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa
     5581 tcccatctcc tgggcaaatt tcttgcggat actgcgtata cggcccaaca actcaccatc
     5641 ctgctggaaa tcgaccatcg ggatcagtcg ataacccact tccattccca gagaatcttc
     5701 cagttgtaca tcgttccacg tcgcttcgac aacggtatta ttctctgcca tttttaccgg
     5761 tttgggttcg gcaggcgctt tttgttcgcg tccgcgtatc caccaggcca gccccagcaa
     5821 tccggcagtg aacagcaaaa ataccaggtt cggcattcca ggcaccaggc cgagtaaacc
     5881 gagcacggcg gcgcttaaca acataacgct tgggttactg aaaagctgat tcaccatctg
     5941 ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg acccccgcgg cggtagaaat
     6001 caccagcgcc ggaatttgtg ccaccagacc gtcgccaatg gtcaatagcg tataactttc
     6061 cgccgcgtgt cccatgctca tgccatgttg cagcacgccg accagcaacc cgccgacaac
     6121 gttaatgacc atgatgagga tcccggcgat ggcatcgccg cgaacaaact tacttgcccc
     6181 gtccattgag ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc
     6241 atcttcacca atcaatccag cgttaaggtc ggcgtcaatc gccatctgct tacccggcat
     6301 accatcgaga acaaagcgcg cacccacttc tgcgatacgc ccggcacctt tggtaatgac
     6361 cataaagttg atgatcacga gaatgacaaa caccacgata cctatagcga aattgccacc
     6421 aacgaggaag tgaccgaacg cttcgaccac cttccctgcc gccgccgcgc cggtatgccc
     6481 ttccatcaaa atgatacggg ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa
     6541 caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag
     6601 caacaccatg atcgacaagg caatattgaa ggtaaacaac aggtcgagta tgaatgcggg
     6661 cagtggcagc accatcatcg acaagatcaa caggatcaaa atcggtccgg caaggatctg
     6721 ccattgtgtc gatttcaggt ttgcgggcag gcgcagcatc gcggtcagat tactcatggg
     6781 tcggtttctc gttaataaaa tccagggctt ccggcaccgg aagatgagta ggttgtacag
     6841 ggcgctgtcc accagccaga cgccagcgtt tcagttgcca gacccaggcc agcacttccg
     6901 ccaccgcggc gtacagttga cccgggattt gttgaccaat ctccgcatgt cgatacagcg
     6961 ctcgcgccag cggcggcgct tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa
     7021 tgcgcagcgc gaccagccct gcacctttag cgaccacttt cggtgcgctc attttgtttt
     7081 cgtcatactg caacgctacc gaatagtggg tcgggttatt gacaatgaca tccgctttcg
     7141 gcacatcggc catcatccga cgccgtgcag cagctcgctg catctgacgg atccgccctt
     7201 taacatgagg gtcaccttcg ctttgtttga actcatcacg aatatcctgc cgtgacatac
     7261 gcagcttttt caggtggctg aatatttgga aaaagacgtc aaatcccacc attggaatga
     7321 caccaagcac caccagcagt gcgcatagcc ctaccaaatc catcgcatta cccatggcgg
     7381 taatcggaga ctcggccatc aagcgcatca tctgcggcca gtgatgccag agaaaaaacc
     7441 ccgtcacgct gccaaccagg atggttttca aaattgcttt aagcaactcc gcgccagtct
     7501 gagccgagaa catccgttta atgcccggta gcgggttgag tttggaaaac ttcggctgca
     7561 aggatttgcc gctaaatacc agccctccca gcatgaccgg agaaatgagc gccaccagca
     7621 ccacgccgct aatcagcggc agcagcgcca gcatggcttc tctgatcagc agaataatct
     7681 gcccgaggat cagattcggg tcattgataa tactgtgatc aaaatgcagc ccagcggaga
     7741 gcatgcccga caatcgacgg gccagcgaca caccgccaaa ccagataaca ctaacgccca
     7801 ccagcaaaat cagcagtgag gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg
     7861 ctttttctag tcggtgaggt gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt
     7921 cgccaatcct ggaaagagta ttaagcgtga atgatgccag agcgcaaagc gttcaatggt
     7981 ttgagtaagg ggcaaaacag gcgggattta gggcttttgc tgccacacat caagcatagt
     8041 gtgcgtttgt cggatgcggc gtcatcgcct tatcagaccg cctgatatga cgtggtcacg
     8101 ccacatcagg caatacaaat caaaatccaa gactatccaa caaatcgtcc acctgatcct
     8161 gactggctac cacaccggct ttgctggtat cgacctgagg tccattaagc aaactctggt
     8221 tttcacgttt tggacgcgac tcctgttccg ggatgttttc caacagcacc atcagcaact
     8281 ggcgttcgat ctcctgaatg acatccatca tccgcttaat gacctgcccg gtgagatcct
     8341 gaaaatcctg cgccatcatg atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg
     8401 gtacatctgc cagaaattgt cgtgtatctg ttaccagttc acgggcgtcg gcaaggtcaa
     8461 tcggatcggc aaaccagtca tcccaacgtt gggttaacgc ttttgctgat ttctccattt
     8521 gatcctgatg cggttgtgac gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg
     8581 tcatctgcac aacatagtac aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg
     8641 caatggcctg atccagcccc agttcccgca aactgtcgcg cagcatacgc gtcaggctgc
     8701 cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc aggtttgatt gatggttgca
     8761 tcatagtcgc atcctcacat gcccagtttc tcaaagattt tgttgagttt ttcctccagc
     8821 gtcgcggcgg taaatggctt caccacatag ccactggccc ccgcttgcgc cgcagcaatg
     8881 atgttctctt tcttcgcttc tgcagtcacc attaacactg gcaatgccga catcgcgcca
     8941 tccgcacgaa ttgttttcag caattccagg ccatccatat tgggcatgtt ccagtcggag
     9001 ataacaaatc cataaccgcc tgcctgcaac ttattgagag cgtcgacgcc atcttccgct
     9061 tcctcaacat tattgaatcc cagctctttc agcaggttac gcactatgcg tcgcatggtg
     9121 gaaaagtcat ccacaaccaa aaatttaagt tctttatccg ccatttcaca ctcctgattt
     9181 aaatacgtat cgcctgtccg gcactaattt ttgccaacat ttgctggctt acctggctaa
     9241 gatcgaccac ttcgcagaca ccacccatat tgatggcctc gcgcggcatg ccgaacacca
     9301 cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc ctgacgcatc gccaacattc
     9361 ccgccgcgcc gtcgttgccc ataccggtca ggatcacccc aaccgcatta cgccccgcct
     9421 gtttggcgac agaatggaac aacacatcta ccgaaggccg atgacggtta accgccgggc
     9481 catcgtgaat tttgatttgg taatttgcgc cactacgcga cagctccata tgccgatcgc
     9541 ccggcgcgat ataggcatgt cccggcaaaa cacgttctcc gtcttcggct tctttaaccc
     9601 cgatctgaca aagcttatta agtctgtcgg caaaagagcg ggtgaaacca ggcggcatat
     9661 gctgggtaat taacagtgcc gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg
     9721 cctcagttcc acccgttgaa gcaccaatcg caatcagttt ttcagaactc aacaacggcc
     9781 ctgccttcag cgttgtcggt gccgacaatg gcttatgtgc tgcaaggctc gcctttgctg
     9841 ctgtacgcac cttttcagca atcatttcgc tatacgccag cattccttcg cgaataccca
     9901 gttgcggttt ggtgacaaaa tctatcgccc ccagctccag cgcgcgcagc gtgacttctg
     9961 accctttgcc ggtcaaggaa gaaaccatca caacgggcat tggacgcaaa cgcattaatt
    10021 tttcgaggaa atccagtccg tccatccgcg gcatttcaac atccagcgtc agcacatcgg
    10081 gattgaattt cttaatcaag tcacgcgcga ccagcggatc aggcgcggtc gccaccattt
    10141 ccatgtcgct atggctgttg atgatttctg tcatgatctg gcgcatcagt gccgaatcat
    10201 cgacagataa caccctgatt ttgctcatcg ttaatcctta cttagcgcat acaccgtctg
    10261 accacgcagc gtgaagcggc gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag
    10321 caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa atctcctgct gggtattttg
    10381 atcgaagtag atcatgacgt tacgacagaa gatcgcatca aacggccccg gcacggtgta
    10441 ctgtttcgcc agtagattca gcggggcaaa atcaacatag ttcgccagct cctgacgcac
    10501 gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg
    10561 cgtcaggttt ttcaactctt catggcgata aataccgctt ctggcttttt ccagcacttc
    10621 ggtgtcgata tcactggcaa acactttcca gcgtccgggc gcggtgccca atgtgtcagc
    10681 cagcgtcatc gcaatgctgt acggctcttc gccggtcgaa gccgccgcgc tccatacgcg
    10741 atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga gggaaatggt gtgcctcacg
    10801 gaaaaatgcc gtcagattcg tggtcagcga attgataaac gcctgccact caccgctgtg
    10861 ctgattagat tccagcaagt tcagataatg accgaaatcc gtcagtccca gcgaacgcaa
    10921 acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc
    10981 tcgttgatag atcaattgac ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc
    11041 ggtcatctgt aacaataaag acgtttgccc acagggcaga gatgaagtca tagcgccttc
    11101 tcaatcactt caggatacca ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac
    11161 ttcatgttct tcaagggtaa ataccgccac acgcgacgaa agatggtcgg cctgattcgc
    11221 cagttgttcc gttgccaccg ccgcttcttc taccagcgag gcgttctgct gagtcacctg
    11281 atccatctgg ctgacagcct gtgcaacctg ctcaatcccc cgctgttgtt cttccgacgc
    11341 agaggcaatt tctcccataa tgtcgttcac gcgggtcacc gaactgacaa tatcgatcat
    11401 ggtcgcggcg gcgttattca ccagtttcga accctgctgg acacgattga ctgactcttc
    11461 gatcagccct ttgatctctt ttgctgcctg cgcgctacgg ctggcaagat tgcgcacttc
    11521 acctgccact accgcaaaac cacgcccctg ctctccggcg cgagccgctt ccactgccgc
    11581 attcagggcc agaatattgg tctggaaagc aattccgtcg ataacgctga taatgtcgcc
    11641 aattttttgc gagctggtgg cgatctcctg catggtgtga gtcatggtac tgacctgaac
    11701 accgcccgcc tgcgccgttg tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt
    11761 atcggcgttt tgccctaccg tggcggttaa ttgctccata ctggcggccg tttgtgccag
    11821 cgatgccgcc tgctgttcgg tacgacttga gagatcgtta ttgcctgcga caatctccgc
    11881 gataccaatg tgcatctcct ggcttccctt acgcacatca cttaccgtcc cacgcaaagc
    11941 ctgctgcatg gtcttcagac tggcaaaaat ggcggtgatc tcattacgac catataccgc
    12001 aatcggacgc gccagattac ccgcagcaat gctgtcaaaa tggctaccga taatggccag
    12061 tggttgaaca atcatcttgc gcgtccacca cagcgcactg ctgatgtaga tcgccgcaac
    12121 aataatcatg ctgataaaca ccagcgccga aatctgatag ttacgctggc tttgcgcact
    12181 ggcggcttcc agcacatggt tgatctccag ctgccaggcc tcaaagttaa cgtcaaacgc
    12241 attctgtgat tcctgcaccg gcgcagtgag gaaatccgaa agttgattac tttcaagcca
    12301 ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa cttttctccg tctctttttg
    12361 caacgccctg acgtgctcgt tgcccgcagt catcgccata aaacttttaa acagcgtggt
    12421 ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata
    12481 gctaagcgcc gtcagagtgc ccgctttgtt cagcgcggta ctggcctgta acattaccgc
    12541 ccgagtttgc gctaatgccg cacgttgctg attgctctgc tcgacctgat tcagtcgttg
    12601 caaatcgtcg cgaaatgccc aaaaagacat gccgttactg ccaatctgca agatcccgca
    12661 gagaatcaaa attaaaaaca gcgtggtcga aattcgaata cgattaaaca tcaacgctcc
    12721 ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa aatgtttccc agtttggatc
    12781 ttgttcagca attcgcagtc gtggctgtgc cggtggttgc tcactggcag gacgggatgg
    12841 tgtttgcggt ttattggtga gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt
    12901 taaacgactc gcctgttctt ccagcgcagc ggcggcggca gctgattcct gcaccagcga
    12961 tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc
    13021 acggctctgt tcatccgatg ccgatgcaat ctcgcccata atgtcagtca cgcgagtgac
    13081 agcattgacg atattgttca ttgtttcccc ggcgctttcg accagcaccg aaccggtatc
    13141 aacgcgtgag acggagtctt caatgagggc tttgatctct tttgccgcct gggcgctacg
    13201 actggcaaga ttacgcactt cacccgccac cacggcaaaa ccacggccct gttcacccgc
    13261 acgcgcggct tcaaccgcgg cattcagcgc gaggatatta gtctggaagg caataccgtc
    13321 gataacgctg ataatgtcgg caattttctt cgaactatcg gcgatctcat gcatcgtttt
    13381 cactacgcca tccaccactt tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag
    13441 ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact gtcgcggtga gctgctccat
    13501 gctggcggca gtttcttcca gcgcggatgc ctgctgttca gtacgggagg aaagatcggt
    13561 gttgcccgcc gcaatttcac gggtaccggc atagatggca tctgaacctt cgcggacatg
    13621 agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat
    13681 ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta ccaccggcga tttcgcgaat
    13741 gtgagcaata atttttgcca gcggagtaag caacatacgg cgaatgccgt accacgccac
    13801 cagcagaatc aataccacca ccagcgcgat aaccgccagt tgccactggg caaatcggta
    13861 atcatctgcg ttgtcagtga cgatatcgcg atacagtttt tcactgctga gggcgtactg
    13921 agcaaacgct tcgcccattg cattttgcat tccctgggtt ggctgagcga aataagctcc
    13981 agtattgcca taatctagat aatcaatcag ttcagttaac gctgtgtaat agtttttata
    14041 tttttcatca atattacgac tggtagcgac catttcaggt aacggtgcca tgcttttgaa
    14101 ttttttataa tgcgtcgctg cctgcgccaa tgttttcctg gcgctatcga gcaattcaac
    14161 tttggcgtta ctttgttgat tggaggaatc catcatcatc cgtaccgctg aacgactcag
    14221 gttaatgcgc gtttgcagca ttaaatccca ggttgacgtc agctcgccct gctgttcccg
    14281 taattgattg gaaaccacaa agctcttctg gctatggtga agggaagaaa aaaacagact
    14341 gccggaaata agctgtaaca gtgcgaatac ccccagcacc attaccaaca gcgtgactac
    14401 gcggatacgg ttaatcataa ggcaccttcc tgaaaacaag ttgatctcgt tatcggcaag
    14461 gaggggggaa actttattgc tgatgccacc cgccgcgaaa ttgaaataaa aaacccgatg
    14521 cgcagatcat cgggttcatt tcaattgagg aaatcgggag aattacgcca cttctgacgc
    14581 cgcgctatct aacagcgcca tctcttcgct gttcagcagt ttttcgatgt tcaccagaat
    14641 caacatccgg tcgcccagtg cgcccagtcc agtgagatat tctgttgaaa gcgtcacggc
    14701 aaattccggt gccggacgaa tttgctccgc cgtcaatgaa agcacgtctg agacgccgtc
    14761 aaccacgatg ccgaccaccc gctgtccgag attcaggacg ataactaccg tgttgtcgtt
    14821 atagtccaca tccacctggc tgaacttaat tcgtaagtca acaatcggca caataacccc
    14881 gcgcagattc gtgacgcctt tgataaacgc tggcgtgttc gcaatccgtg ttacctgatc
    14941 gtagccacgg atctcctgca ctttcaggat atcaataccg tactcttcat caccaagggt
    15001 aaataccaga aattcctggc ctgacggctc gctggccagc tttgttacat tcgtcatacc
    15061 ggtcatattg ttaccttttt actcattcag gcggcggtgt tcgccatacg ttgttcgcgg
    15121 tttatcgcct gcaaggcgga gacatcaaca atcagtgcca cgctgccgtc gccaagaatg
    15181 gtcgcagcag aaatgccggg gactttgcga tagttacttt caaggttttt aaccacaacc
    15241 tggtgctgac caattaattg atccaccagc aaggcgtagc ggcgaccgcc actttgtaag
    15301 atcaccacaa ttccctgggt ggcttcggtt ttcgcgcccg cgacgttgaa cactttccac
    15361 agttcgacga tgggcagata ttcaccccgc acttccagca ccctctcgcc gccggccagt
    15421 ggatgcagat cggcttcacg gggttgcagt gattccataa cagcattcag cggcagaatg
    15481 aaaacttcat ccgcaacgcg tacggacatg ccgtcgagga tggccagcgt cagcggcagt
    15541 aaaatgcgga tcgtagtgcc agtaccctgc ttcgactgga tttcgacatg accgcccatc
    15601 tcctggatat tacgtttaac gacgtccatg ccgacgccgc gcccggagac gtcggtgacc
    15661 tgctctgccg tggagaagcc tggtgcaaat atcagcatcg cgacttcgtc gtcgctcatg
    15721 ttttcgctga cagtcaaacc ttgcgaggcc gcttttgcca gaattcgctc acggtttagc
    15781 cccgccccat cgtcggtcac ttcaatgcaa atgttgccgc cctgatgttc ggcagacaga
    15841 attaaatttc cgacgctgtt tttacctgcg gcgagccgtt tttctggcag ttcaataccg
    15901 tgatcgaggc tattgcgtac caggtgggtc agcgggtcga taatgcgttc tatcaggctt
    15961 ttgtcgagtt cagtagaact gcccaccagc gtcagttcta cctgcttgcc gagttttccc
    16021 gccagatcac gcaccagccg gggatagcga ctaaaaacat attccatcgg catcatgcga
    16081 atcgacatca ctgattcctg caaatcacgg gcgttacgtt gtaactgccc catgctggtt
    16141 atcaaatcac catgattaac cgggtccagt tcgctggaac gctgggcaag catggactgg
    16201 gtgataacca gctcgccgac gaggttaatt aattgatcaa ccttttctac cgctacacgg
    16261 atgctggtgg attcattgct gcgcgtcgtt ttttcccgct ccacgcggcc ggttggcgct
    16321 tgttcggctg ccagtttaag cactggtggg gtggatattt ttggcgagac ttctactgtt
    16381 tcaaaggtaa tctgatcggc ttcaatcaca aaacagagta ccgctgtgat gtcatcttcg
    16441 gcgatgtcgc ccggtaatat tgccgagagc gaatccgccc ctttcaccac gtcagttaac
    16501 gttgtcagat gtcccagttc ttcttccagc aggtcgactt ccccggcctt caggcgcgaa
    16561 aggataattc gtcgcggcga ctgactgcga ctctgctcat cttgcggttc acttttggca
    16621 accacactta atcgggtcac tgcggatggc gtttcgcctt tcgcttctaa tgccagttga
    16681 cgcaaggcct ggcagatata atcgaagctg gcggcatccg gctcttgcga ctgtttataa
    16741 gcgtcgagct gttcttgcat gatgtccttc gtttccaaaa acagattgat aatgtcggtg
    16801 ttgagttgca tctcacctcg tctggcttca tcgagcaggt tttccatcag atgcgtggtt
    16861 tcctgcaaaa cgctgaagcc aaaagttcct gcccctcctt tgatcgagtg ggcagcccga
    16921 aagatggcat tcaattgttc ggcatctggc gcttccggct gcaaaaccag cagatgctgc
    16981 tccatatcag ccaacagttc gtccgcttca tcaaaaaatg tctgataaaa atcgcttata
    17041 tccatgctca cgctgtcacc tcggttcggc tgatggcatt gtgggaacac tgacctgtgg
    17101 tgcaacctca ggtttttcca gggcgcttac tggctcattc tggctttcgg cgttttcatg
    17161 caaaatggcc tgttcggctt gtttgttcag taccagcagg ctgatgcgac ggttgacggc
    17221 atcatcaggt ccgcgatcgc ttaagcgcat cgttgccgcc atgccgacga cacgtaacac
    17281 tttgccgcta tccaaccctc cgaccatcag ttcgcggcgg gatgcattgg cccgatcggc
    17341 agaaagctcc cagttgctgt aacctttctc accactggcg tatgggaaat catcggtatg
    17401 acccgaaagg ctaatacggt tgggaatacc attcagaacg ggcgcgatgg cgcgcagaat
    17461 gtcgcgcata tagggttcga catcggcact gccggtttta aacatcgggc gattctggct
    17521 atcgatgatc tgaatacgta gaccttcctg gaccagatcg attttgagat gtggacgtaa
    17581 cgcccgcagt ttcggatcgg actctatcaa ctggtcgaga tcaccccgca atttccgcaa
    17641 tcgactttgc tccatgcgtt ttttcaactc ttcgatgttc ggctgcttat tcacttcccc
    17701 ctggctttgg gtgtaatcat caccaccgcc gggaattggg ctttcactat tagaaatgcg
    17761 atcgccgccc gtaaccgctg tcgccagtgg agtccggaag tactccgcaa tctgaatcag
    17821 ctcttttggg ctggagatgg agatcagcca catcaccaga aaaaaggcca tcatcgcagt
    17881 cataaagtcg gcataagcaa tcttccacga tccatgtgct gccccgtggc ttttggcttt
    17941 gcgtcgtttg acgacaataa tcggatgcgc ttgattcttc atgcttcctc ggttgtcgtc
    18001 tgttgttgcg gatttttcac cgcacgcaca tgctcttcca gttcaataaa cgacggacgt
    18061 tcgctggaat agagcgtttt gcgaccaaac tcaacggcga taggcggtgc gtaaccgttc
    18121 agattagaaa gcagagtgac tttgacgcac tgcatcattt tgctggtttc ggcgcttttc
    18181 tgacgtaaaa cactcgctaa tggggaaata aatccgtaag ccaataaaat gccgaggaaa
    18241 gtccccacca tcgcatgtgc gataagcgca cccagctcgg cggcaggacg atcggctgaa
    18301 cctaacgcgt gaacgacccc cattacagcc gcgacaatac caaacgccgg aagtgagtcc
    18361 ccgaccagcg ccagactgtt cgccgggact tctgcctcgc tttcgtgcgt ctcaatctct
    18421 tcatccatca gggcttcgat ttcgaaggtg ttcatatgac cgctgataat cagacgcaga
    18481 taatcgacga taaaatcaag catgacgcta tccgcgagga tgcgtgggta gctgacgaag
    18541 atctcgctct cacggggatt ttcaatatca cgttccagcg aaaacatccc catctgccgc
    18601 gatttcgcca tcaaccgata aagcagagcc agcagatcca tatacattgc tttggtgtat
    18661 ttggagcgac gaaacagcaa cggcagcgcc ttcagcgtgc ctttaatcgc tttgccatta
    18721 ttgccgacga taaacgaccc aatccctgca ccggcaataa tcaccagttc agcgggttga
    18781 tagagtgctc caaggcttcc accggtcatc aaataaccgc cgaaaactgt accgagaaca
    18841 accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat
    18901 gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac
    18961 cgctgctgga atgttgcgcc tcaccgtatc agttaaacag cctgtactct ctgttcatcc
    19021 agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg
    19081 gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata
    19141 aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc
    19201 accaatgtcc aggcacgggt taatgccagc agtggtcctt cttctgcttg tgggcactgt
    19261 tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa accggttttc
    19321 agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaggtcata
    19381 aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta
    19441 taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga
    19501 gcgcccaggg tgatcaattc cattgccagc tgaatatccc gcgcttcctg aacaatgctt
    19561 ttttcactca tgatcaggcc cttttcttgc gcagcgcttc ttcaggctga ttaacatcat
    19621 tcagcaagcg tgttgagagc atgatgccgg tatgaatttg ctggagatcg tcaacgcggg
    19681 aatcttgcgt caactgagta atcgtctggt ggctgtcaaa acggaagtga caaaccagtt
    19741 gattggtttc tgccagctta accatttgcg gaagagtcag tgccgctaac gttgtcgcca
    19801 tttcttcatt tatgccgaga cgaaacatag cggacgcttt gtcctgaaca atcaaacgct
    19861 gtgcaagtag taaatatgac aagttgatgt cataaatgtg tttcagcaac tcggaggtat
    19921 gcattattcc cacccagaat aaccaacttt atttttatgc ggtttcaccg caccccgtga
    19981 tgtcgccggg aagccccggt aaaaaataat tagcattaga atagttgcga taagctgcaa
    20041 taagcagaac cacctttttg gtttaatatg tccttacaaa tagaaatggg tctttacact
    20101 tatctaagat ttttcctaaa tcgacgcaac tgtactcgtc actacacgca catacaacgg
    20161 aggggggctg cgattttcaa taatgtgtga tgcagatcac acaaaacact caattactta
    20221 acataaatgt ataagtcata cttttgtttt gggtgtattt caatctgtta aaaagttttt
    20281 cgctacgcta gcaagcaaaa atgaaacagg aataatcgaa atgggatgtt gcacacagtt
    20341 aaaataactc accgtaaata atcatctgct ataaataatc actttcatgc aataccagat
    20401 aagctatttt taaacagaca cttaccgcac aacaaactaa ttaaatacaa agaaaggcaa
    20461 caatcaactt aatgttaata aaatgttttg catatattaa ccacttgttt aatgcggaga
    20521 atcagtacac tattttctta taattacatt tgaaattata tgatcaccgg gagtgattga
    20581 ttaattgtct ttactaataa tcagactaat atttacctgt ttgaccgagt tgggattgcg
    20641 tcgtttctcc attaggagta aagctttaat gtcacctgaa gttcacagaa taaggaacag
    20701 gttatgagct atagcaatat tcttgtcgct gttgcggtta caccggaaag tcagcaactg
    20761 ctggcaaaag cggtatctat cgccaggcca gtaaagggag acatcagttt aattactctc
    20821 gcttccgacc cggaaatgta caatcaatta gccgcgccga tgcttgaaga tttacgcagc
    20881 gtgatgcaag aagaaacgca aagctttctt gataagttaa ttcaggatgc ggggtatccc
    20941 gttgacaaga cgtttattgc ctacggtgag ttaagcgaac atattctgga agtatgtcac
    21001 aaacatcatt tcgatttggt gatttgcggt aatcacaatc acagtttctt ttcgcgagca
    21061 tcctgctcgg cgaaaagagt gattgcctca agtgaggtcg atgtgctgtt agttccactt
    21121 acgggagatt aaccgctcct acgcaagctt tggaaaggta gcaactttat cgcgctgctg
    21181 gctttccgcg cttcgcggaa ctatctgctt taggtcgcta atgaagcact cctgccagtg
    21241 gttaatatcg tttttcacga taacgtccag catttctgca tgacgggaaa tacgttccgc
    21301 cagcgacata gtcaatgcac gatccagcgc agctgcaact tcgtcacgat cgtaggggtt
    21361 aacaattaac gccgacgtta actcgtttgc cgctcccgca aattgcgaaa gaacaagaac
    21421 gcccgggttg gctgggtcct gagcagcaac atactctttt gctaccaggt tcatcccgtc
    21481 acgcagtggc gtcactaagc ccacgtcaga gtagcggaat attttcatca gtaatttacg
    21541 gtcaaaatgc tgattcaaat aataaagcgg cgtccagcct aattgcccgt atttaccatt
    21601 aattcgtcca gcttcatttt cgagctgatg acgaatatcc tgataggctt gcacatcacc
    21661 acgcgacgtt ggtgcaatct gggtataacg aattttacca tgatgctgcg gatatttttc
    21721 cagcaacgct tcataggcga gaaaacgctc tggcaaacct ttggaataat ccagccgttc
    21781 gacagaaaag atattttgta cgtttttcag ttccgcttta agttgcgcca gttttggcgg
    21841 cagtggcccg gcagcctgtt tggctatttc tttcggttca atgccgatcg ggtagacttc
    21901 tgttcgaaat gcttttcccc aggctgtatg gctttttgcg ctacgtgtcg tgacgcgggt
    21961 caggttagaa aggcaatcca ggaacgccag acgatcgttt tctgtctgga aacccagcaa
    22021 atcataatca caaagctgtt caagcaaggt gtcatatgtc ggcagcgcgt tgaagatttc
    22081 cggtgtcggg aaaggaatat gcaagaagaa accaatgcga ttattcactc cccgtttgcg
    22141 taattcatgc gcaaatggca acaggtgata atcgtggatc cagataatgt catcgtcttg
    22201 caacagcggc agtaatttat ctgccagcaa cgcatttacg cgtagatagc cgtcccaggc
    22261 aggacgctga aattgcacca gatcgagccg ataatgaaaa gcgggccaga gaacggcatt
    22321 ggagaattgg ttgtagtatt cgtcaaggtc ctgttcgctg aggttaaaag aggcccacgt
    22381 aatgttacct tttttcacct tttttagcgg ctgatcctca ttccctgttt caccactcca
    22441 gccaaaccac agtccgcctg cggctttcag tgcccccagt atgccaacgg caaggccacc
    22501 ggcactggcg gcgtgctcgt ctggtggtgc aatccggtta gatactacga ctaaacgact
    22561 catagtcatc actcctgtta ttttctcttt tttgttgtaa tgcggtggtt atcatttcaa
    22621 gccagctcca gacatccggc acacccgcca gtcgccatga tgcctgagtt gcacctgtgc
    22681 caatttttac tgacattccg cccagccgat taacgactgc gaagccagat tcatcggtta
    22741 aatcatcgcc cagaaatacg ggcgttcgcc cgataaaggg agcttcctgc ataaaggctg
    22801 caatcgcttc acctttactg gtacctctcg gtttgatctc gacaacacac tttccctgct
    22861 gtaacgccat ttgtggccag atctgagtaa tacgttgcgc taatgtcatt aatgcgtctt
    22921 catgctgcgg agcctgacga taatgcagcg caaaagccat ccctttcgtc tccagctccg
    22981 caccgggata ctgggcgatg accgtatgca gttgcacgct aatatcacgc gcaatcgcat
    23041 ccggcagatg aacgatatgt gttttaccat tgatgtcacg gcgctccgcc ccatgcacgc
    23101 ccgctaacgg gaagcgataa ggttttgcca gtgcgtcaag ctccaccatt gagcgccctg
    23161 atatcaatgc caatgcacca tcacttgcgg ttgccagtag ctgtagtcct tgcagaatat
    23221 tgtcaggcac gacgacctga tcgggatgcg gtttgatttc cgccagcgtt ccatcgagat
    23281 caaaaaacca ggcatatttc gcggatagtt caggggtttc ggttaacggt tctgtcaccc
    23341 ggttctcctc cttctctttt cttattgtgt tgcgcagaac gcgctttaca gacatgtaag
    23401 tatagacagt gtgacggggg tcgccatttg caatgaagaa cagccaaccg ttgtggcggc
    23461 tggctgtggt gggaaaaaac gttaaattgt tgtggaaaaa agcatcagac agtgcgtttc
    23521 gctttttgct tgtaacggtc gaagatcacc gctgccagca ggattaagcc gcgaaccacg
    23581 tactgcgcga aaggagaaat attaagcagg ttcatggcgt tttccacggt gcctaaaatt
    23641 aagatacccg ccaccacata tgagattttt ccgatgccac ctttcagaga aacgccacct
    23701 aaaacgcagg cggagataac aataagctca taaccaatcg acgtcattgg ctgcccacta
    23761 gtcatacgtg aagccagaat aattccggct atcgcagata ccaggccaga gagaacaaag
    23821 ataataattt tggtgcgaac aaccggtaca cccgccagac gcgcagcctc ttcgttcccg
    23881 ccaatcgcca gggtgttacg accaaaggtg gttttattca gcaacaaacc aaagataatc
    23941 agacacgcga cggtgagcca gattggcgca ggcagaccga accagttagc gtaaccaagg
    24001 gcaaagaagc tttcatcttc gataccgacc gctttaccgt ctgaaatgat gtacgccaga
    24061 cctcgaacaa tctgcatcgt tgccagtgtt gtgatcagag catttatttt cagtttggcg
    24121 ataacaaagc cattgaccag gccacagaga atgccaagca gcaaccccgc tgccacgcca
    24181 atccacaggc tttcagtcag gttgataacc accgccgtgg tgacacccgc acaggcaatt
    24241 acggaggcga cagaaaggtc aaagtcaccg gaagcaaggc agaacaacat gccacacgcc
    24301 accatccccg acatggaaat tgccaggccc aaccctttca tattaatgaa ggtggcaaaa
    24361 tttgggacaa aaatggcaca ggcgataaag agcaccgcaa acaccaccag catgccgtac
    24421 tgatcccaga tacgcccgaa gctgaatgac gacttaggtg cgccagaccc cgatgtagaa
    24481 acagaagaca tcatactctc cttactcagg caactgcctg gctgacttta ggcatcgcaa
    24541 ggctcagtgc ctgacgctca tctgcctgct cgtgtaacaa ttcaccggcg atttcacctt
    24601 cccgcatcac cacaatccgg tcggcaacgc cgaggacttc aggtaagtcg ctggaggcaa
    24661 acagcaccgc cacaccctgc gccgccagcg cataaatcac gttgtaaatt tcatgcttag
    24721 caccaacatc aatgccgcgc gtaggttcat ccagcaaaat gaccttcatc tcttccgata
    24781 accagcggcc cagaatggct ttttgctgat ttccgcctga gagattcatg atcagttgct
    24841 cagcgcccgg cgttttgatg ttgagcgaac gaatgtggtg atcggcattg ttttcttccc
    24901 aaccgttgtt gattacacaa ccgccgagca catgtttatg tctggcactg atgttgatat
    24961 tgtcgcgaac ggagtgcacg ggaataatgc cttccgcttt gcgatcttcc gggcagagca
    25021 tcatgcctgc ggcaatggcg tggctcggtt tacgaatatc gatcggctgt tggtcgatat
    25081 aaacctgacc ggcggtgatt tgcgtcccgc caaacaagcc tttcattaat tcgctacgcc
    25141 ccgcccctac cagaccaaac agcccaacaa tttcaccact gcgaaccgcc agacttattg
    25201 gcgtacgcac gcctggtgct ttcacagcat caagacgcag gcgctcctcg ccataactac
    25261 gaggttgcca gccgtagata tcgccaatgt cgcgcccgac catcgcctgc accagcgcgt
    25321 cgtggtcaac ctgctgcata tcggtaaagg ttttgacata acgtccatct ttaaagacag
    25381 tgatggcatc gctgagggca aatatttctt ccatacggtg agaaacgtat aagatcaccc
    25441 gcccctcttt tcgcagttca cgaataacgc ggaaaagatt gtcgatttcg cgggcagaaa
    25501 gggagctggt tggctcatca aaggcgataa ttttagcgtt acgcgccagc gccttggcga
    25561 tttcaaccat ctgccactga ccaatggaga gatatttcag cggcgtgtcc gggtcaatat
    25621 ccataccaag atgtttaagt tgtaaacccg cctcataatt cagcaatgag cgattcacaa
    25681 tgccgccttt atgcggcagc tggccgagat agatgttttc cgcgacggtc atttccggca
    25741 cgagatgtag ttcctggtaa ataatcgcca cacccgcgtt aagtgctgcg gtcgtgtcgg
    25801 aaaaggacat ttcctgccca ttaatcacta cagaacccgt ggttggcgca tagttgccgc
    25861 tgaggatttt taagagagtt gattttcctg cgccattttc acccatcaac gcatgaacct
    25921 gaccggcata gcagtcaaaa ctaatatccg tcagcgcctt aacgccggga aacgttttac
    25981 cgatgccgcg aaatgagaga tacggggtag actgttgcat aacgtctccg tgaatcactg
    26041 gtcgttcaca ccccctctgc atcatgcaga ggggaatttt tccggcaaat tacttaccgc
    26101 ctaaaccttt tttctccagt tcttctttaa agttgtcacg cgtgatcagt accacgtcgg
    26161 taacttcggt aaattttggc ggttcaacgt cttttgctac ccagttgtaa agcatttcgc
    26221 tggatttata gccatgtacg tccgggcttg gcagcaggga accgtagaag ccggttgcct
    26281 gtgctttaga cagttcgctc accgcatcca caccgttaat gccaatgccg atgatatcgg
    26341 ccgctttaaa gccctgacct tccgtcgcgc gtacgccgcc cagcacggtg ctgtcgttca
    26401 taccgacgat cagccaatgt ttaacttccg gatgttgaac cagcattgag ttggcagcgt
    26461 caaatgcccc cgggatgtcg ttagatttgg taggtacctg ataaatttgt ttttccggga
    26521 atccggccgc tttcagcgca tccatagatc ccgtagtacg gcggcgggcg gtatccagtt
    26581 cgttggcggt aatcgccatc accgcgcttt ctttgacatc ccagccacgt ttctgcatct
    26641 ctttatacag ttcctggccc tgacgttcgc caattttagt cgccgccatc atcaccagcg
    26701 gaacggtatc cattggctta cctttggcgt taacaaactg gtcatccacg gcaatgactt
    26761 tcatatcgta gccacgcgct ttcgcgacga tggcagagcc gagtttgggg tccggagtac
    26821 aaataacgaa accttttgcg ccactggcag ccaggctgtc gatcgcgttc aatgtttttt
    26881 cgccatccgg cacggcaatc ttaataacct caaaccctaa atccttcccg gctttatcgg
    26941 caaacttcca ttcggtctgg aaccacggct cttccggttg cttcaccaga aaaccgagct
    27001 tcaggttctc cgccatagcg gattgtgaca taacggctgc cagaccaatg gctgccaggg
    27061 ctttagtaaa tttgtgcatg gttctctcca gctttagtgt cgttttgtgt agggcaaaaa
    27121 cgaatgacat tcgttaaatt aatcggaaaa caaagcatta ccttttaact aaaagataag
    27181 tgaccgtgtt gacatagttt tagcgagaaa ttaattctcc ataggagagc aatatcacat
    27241 cgcagaatta cagtgagaac gtgcataaat ttagcgggaa aagacataag ggaaagccaa
    27301 tttgtcagac aaattgtcga atgcacagca gattaatcca taagattagc ctggaaatcc
    27361 ttgttgtctt tggtacccat gcgggatgtc ttctttttaa ccagtcaata ggccgcatta
    27421 cctggcgttg agtttttgaa atggtgtaat aaccgcaact caaagatgtg gaaaatgcac
    27481 gtcattcatt tcgtcattaa ttatcactgt gct
//
LOCUS       DAZGXZ010000063        27329 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000063 DAZGXZ010000000
VERSION     DAZGXZ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.063"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..752)
                     /locus_tag="ACHKJF_002754"
     CDS             complement(<1..752)
                     /locus_tag="ACHKJF_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0950087.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYL"
     gene            760..1191
                     /gene="tnpA"
                     /locus_tag="ACHKJF_002755"
     CDS             760..1191
                     /gene="tnpA"
                     /locus_tag="ACHKJF_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP0950088.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(1207..1395)
                     /locus_tag="ACHKJF_002756"
     CDS             complement(1207..1395)
                     /locus_tag="ACHKJF_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP0950089.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(1399..1758)
                     /gene="yeaR"
                     /locus_tag="ACHKJF_002757"
     CDS             complement(1399..1758)
                     /gene="yeaR"
                     /locus_tag="ACHKJF_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP0950090.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(1931..2569)
                     /gene="leuE"
                     /locus_tag="ACHKJF_002758"
     CDS             complement(1931..2569)
                     /gene="leuE"
                     /locus_tag="ACHKJF_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP0950091.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(2696..3619)
                     /gene="dmlR"
                     /locus_tag="ACHKJF_002759"
     CDS             complement(2696..3619)
                     /gene="dmlR"
                     /locus_tag="ACHKJF_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP0950092.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            3722..4807
                     /gene="dmlA"
                     /locus_tag="ACHKJF_002760"
     CDS             3722..4807
                     /gene="dmlA"
                     /locus_tag="ACHKJF_002760"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP0950093.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDGRFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            5058..6668
                     /gene="yeaV"
                     /locus_tag="ACHKJF_002761"
     CDS             5058..6668
                     /gene="yeaV"
                     /locus_tag="ACHKJF_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP0950094.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            6700..7824
                     /gene="yeaW"
                     /locus_tag="ACHKJF_002762"
     CDS             6700..7824
                     /gene="yeaW"
                     /locus_tag="ACHKJF_002762"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP0950095.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGGGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            7880..8845
                     /gene="yeaX"
                     /locus_tag="ACHKJF_002763"
     CDS             7880..8845
                     /gene="yeaX"
                     /locus_tag="ACHKJF_002763"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP0950096.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNHYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(8899..10014)
                     /gene="rnd"
                     /locus_tag="ACHKJF_002764"
     CDS             complement(8899..10014)
                     /gene="rnd"
                     /locus_tag="ACHKJF_002764"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP0950097.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKVFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(10096..11781)
                     /gene="fadD"
                     /locus_tag="ACHKJF_002765"
     CDS             complement(10096..11781)
                     /gene="fadD"
                     /locus_tag="ACHKJF_002765"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP0950098.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(11986..12567)
                     /gene="yeaY"
                     /locus_tag="ACHKJF_002766"
     CDS             complement(11986..12567)
                     /gene="yeaY"
                     /locus_tag="ACHKJF_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP0950099.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(12607..13302)
                     /gene="tsaB"
                     /locus_tag="ACHKJF_002767"
     CDS             complement(12607..13302)
                     /gene="tsaB"
                     /locus_tag="ACHKJF_002767"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP0950100.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(13360..15270)
                     /gene="yoaA"
                     /locus_tag="ACHKJF_002768"
     CDS             complement(13360..15270)
                     /gene="yoaA"
                     /locus_tag="ACHKJF_002768"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP0950101.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            15402..15746
                     /gene="yoaB"
                     /locus_tag="ACHKJF_002769"
     CDS             15402..15746
                     /gene="yoaB"
                     /locus_tag="ACHKJF_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0950102.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANKLA
                     QIDAVLEKHGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            16168..16467
                     /gene="yoaC"
                     /locus_tag="ACHKJF_002770"
     CDS             16168..16467
                     /gene="yoaC"
                     /locus_tag="ACHKJF_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP0950103.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(16587..16766)
                     /gene="yoaH"
                     /locus_tag="ACHKJF_002771"
     CDS             complement(16587..16766)
                     /gene="yoaH"
                     /locus_tag="ACHKJF_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP0950104.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            16840..18201
                     /gene="pabB"
                     /locus_tag="ACHKJF_002772"
     CDS             16840..18201
                     /gene="pabB"
                     /locus_tag="ACHKJF_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP0950105.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            18205..18783
                     /gene="nudL"
                     /locus_tag="ACHKJF_002773"
     CDS             18205..18783
                     /gene="nudL"
                     /locus_tag="ACHKJF_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP0950106.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            18967..20331
                     /gene="sdaA"
                     /locus_tag="ACHKJF_002774"
     CDS             18967..20331
                     /gene="sdaA"
                     /locus_tag="ACHKJF_002774"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0950107.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            20462..22060
                     /gene="pdeD"
                     /locus_tag="ACHKJF_002775"
     CDS             20462..22060
                     /gene="pdeD"
                     /locus_tag="ACHKJF_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0950108.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRNNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(22064..23620)
                     /gene="yoaE"
                     /locus_tag="ACHKJF_002776"
     CDS             complement(22064..23620)
                     /gene="yoaE"
                     /locus_tag="ACHKJF_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP0950109.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLSVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(23608..23766)
                     /gene="yoaL"
                     /locus_tag="ACHKJF_002777"
     CDS             complement(23608..23766)
                     /gene="yoaL"
                     /locus_tag="ACHKJF_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP0950110.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            24083..25054
                     /gene="manX"
                     /locus_tag="ACHKJF_002778"
     CDS             24083..25054
                     /gene="manX"
                     /locus_tag="ACHKJF_002778"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP0950111.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            25117..25917
                     /gene="manY"
                     /locus_tag="ACHKJF_002779"
     CDS             25117..25917
                     /gene="manY"
                     /locus_tag="ACHKJF_002779"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP0950112.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            25930..26781
                     /gene="manZ"
                     /locus_tag="ACHKJF_002780"
     CDS             25930..26781
                     /gene="manZ"
                     /locus_tag="ACHKJF_002780"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP0950113.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            26836..27294
                     /gene="yobD"
                     /locus_tag="ACHKJF_002781"
     CDS             26836..27294
                     /gene="yobD"
                     /locus_tag="ACHKJF_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP0950114.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         6658 a         7065 c         6791 g         6815 t
ORIGIN      
        1 tgaaggtagt ccctgcggat atttgcgata cgggaatgca gtcgctgtat tttgcgtttc
       61 tgcttctgcc agttgttgct gaatttgacc ttgcggctta actgtcgctg aagtctcgcc
      121 agcgtcttct ggtttttctg aaaactgttt acaggctcaa agactgtgcc atctgacagc
      181 gtggcgagtt tagccacgcc agcatccagc ccgaccattg atgctgaagg gtgaaccgga
      241 gtggatactt cactttctgt ctgaatactg atgtaccact taccgcagga ctggctgaca
      301 gtgacatttt tcacaacacc cgtgacctga cggctattgc ggtagcgcat ccagcccagt
      361 ttcggcagaa aaatacggct gttttcctga tcgagcttaa cgccctgcgg gtagcggaat
      421 gcatcattct gtccccgctt tttgaatcgg ggaaaagcag cccgcttccg gaagaagttt
      481 ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg gctgtgaggg agaatcttta
      541 agccattgcg tttcagtggc atttttccac tcaaccagcc aggaagccat tttcccgtaa
      601 gggatgtatt tattaccggc ctcatgattc tcattctgaa gcgccagagc acgattgaaa
      661 acgaaacgac atgcgcctgc gaagcgcctc atttcacact cctgttgatc acctggtctt
      721 aactggaatt taaatgcctg tagtcgtttc acaggccaaa tgatactttg gtctgtgaaa
      781 aaagaaaccg atatccggcg tggaaggcat tgtgttttcc tgatgcatgt tcacctggtc
      841 tttgtcacca gatacagacg tcagattttt gaccatgacg cgacagaaaa actacgcgct
      901 tacttttcaa atgtatgtgc tgattttgaa gctgaacttg ttgaaatgga tggcgaacca
      961 gatcacgtcc atttgttaat taactatcct cccaaactgg cgatatccag tctggtaaac
     1021 agcctcaaag gcgtatcggg taggttactg cgacgagatc gaccagatat tgcagtcagg
     1081 tattactaca aaggcgtttt gtggagtcct ggctattttg ccagtagctg cggaggtgcg
     1141 ccaatatccg tcatccgcca atacattgaa caacagcaaa cacctggtta ggtggaaaac
     1201 cgcgccttat atccccgccc tgaaggacgg ggttttacgg cgcaccggat aagaacgcac
     1261 ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca aagtcatcgg
     1321 cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg tcacggtata
     1381 cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt gcgcaccttc
     1441 catcaggact tcaggagcca cgaagaagtc aatgttgaaa taagtatcgt cagtcatggc
     1501 ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt cgataaggat
     1561 cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga ccgccccatg
     1621 cataacggaa aggcgtgggt aaacccccgg gcgcgttcct ttatcaagat gacgttcgaa
     1681 tattccggca ggcgcagttt gtttattcca gaaaggcgtt gagcgcgtat gaatataatt
     1741 ctgtgggatt tgcagcatcc tattccctcc ttcggggaat gcgctgaaaa cggtttattc
     1801 cagccgtttc agggtacgcc tgataatttg cattttaaat accatttatt ggttactttt
     1861 tagcaccata tcagagaaaa atcagggagg attacaagtg ggaagcccgc gacaacgcgg
     1921 gctgaatgta tcaggattgc agcgtcgcca gtcgggcggc gaaacccacg aacatcaaac
     1981 caatcagtga gttgccaact ttagccagtt tctttttggt acgtatgtac tgcgtgacaa
     2041 aagcaccaga aataatcagg aagctcaaat agcagaaact caccagttcc agcgtcgtcg
     2101 ccagaataaa gaatgaaatt cccgtatgtg gggcattaac atcgataaac tgtacgaaaa
     2161 acgacacata gaacaaaatg gctttcggat tagtcaggct caaaattaac gcgcgtttaa
     2221 aaatggcccc gtattggggc tcatcggatt tggcctcgtt atttttaccc ttcagggtcg
     2281 cgtagagaat tttactcccc agatagagca aataaaacgc accaagataa cgtacaatgt
     2341 tgaataatat cggggtggtc ttaattaatg tcgccactcc agcccatgcc agaaacatca
     2401 atactgcatc gccaataaat acaccgcagg ccgcaagata accgcctttc ataccgctac
     2461 tgacgctatt tttgagtaca aacagggtat ttggccctgg caccaacaca ataaaaatgg
     2521 ccccaaccag ataggtccag taattcagaa ccccgtattc agcgaacaca ttaacctctt
     2581 taattatctt tcgatcatgc gcgattaaag gtgaatatgc taaccaatct gtagcggctt
     2641 agaaaggaga aaatcaggtt ttaacccgat atcaacccga taattgaatc attaatcagg
     2701 catgctccag tgaaaaattc gggtagtgct cggcaaaata ctggcgtaaa aattctaccg
     2761 ttatccgcac tttcgctgac gtcgccagcc ttgaaacata aacggcccag acgttcgctg
     2821 gctggtaata ttccggtagc acttgcacta aatgaccact ggcaatgttt tcgctaacat
     2881 cccaccagga gcgcagagca atcccctgcc catcgagaca ccactgatgg acaatttctc
     2941 catgattaga cgacagtggg ccggtgactt taatcgcatg gtggccttct ttattgcgta
     3001 attgccagac accaaaagga tgatcgcgct ctttaatgac caggcacggc aatgcggaaa
     3061 gatcggttaa atgtttcggc gcgccatgtt gcgcaataaa ttccggcgac gcgcagagaa
     3121 tgcggtaatt agtcgccagt ttgcgggcga ttaaattagg ggcgatgtca tcgccaatgc
     3181 gaatatcgag atcaacacct tcattgacca aatcgaccaa ccggtcttcc acatcaaaac
     3241 gtaattccag ttgcggatac gccttagcca gcgctgataa cgccggagcc accacctgtc
     3301 gtccaaaacc aaagctgctg ataatacgca gcatcccctg cggcacctga cgcacgtcag
     3361 aaagttcgtc catcatctga ccgacatcct gcaaaatccg ctgcgcccat tcataaatcc
     3421 gctctccttc ttcggtaatg gtgacgcggc gggtggtgcg gtgtaacaac accacgttta
     3481 gcgtttgctc cagcaaggcg atgcgcttgc tgacgaacgc cggtgaaacg cccagttctt
     3541 ccgccacggc ggcaaaacca gcccgacgag ccaccagcat aaagacgcgc aaatcattca
     3601 gcagcggtaa attattcatg attcgtgttt tatgtttcac cagttacggg gattaattcc
     3661 ttttcagtca attataggat ggtgatgttg tcaattttga tggtcaggaa gtgagaaccc
     3721 aatgatgaaa acgatgcgta ttgctgcgat cccgggagac gggattggca aagaagtcct
     3781 tcctgaaggg attcgcgtgt tacaggctgc cgctgagcgc tggggcttcg ccttgagttt
     3841 tgagcaaatg gagtgggcga gctgcgagta ttacagccat cacggtaaaa tgatgccgga
     3901 cgactggcat gagcaactta gccgtttcga cgccatctat tttggtgccg tcggctggcc
     3961 ggacaccgtt cctgaccata tttcgttgtg gggttcgctg ctgaaatttc gtcgtgaatt
     4021 cgaccagtac gtcaacctgc gcccggttcg tctctttcct ggcgttccct gcccgctggc
     4081 gggaaaacag cctggcgaca tcgattttta cgtggtcagg gaaaacaccg aaggcgaata
     4141 ttcctcgctc ggcggtagag tgaatgaagg tacagagcat gaagtcgtca ttcaggaatc
     4201 ggtatttacg cgtcgtggtg tcgatcgcat tttgcgttat gccttcgaac ttgcgcaaag
     4261 ccgcccacgt aagacgctaa cttctgccac taaatcaaac ggtttagcca tcagcatgcc
     4321 gtactgggat gagcgagtgg aagcaatggc cgagaattac ccggagatcc gctgggacaa
     4381 gcagcatatt gatattctct gcgcgcgttt tgtgatgcag ccggaacgct tcgatgtggt
     4441 ggtggcgtcc aatttatttg gcgatatcct ttccgatctt ggcccggcct gcaccggcac
     4501 cattggcatt gccccatccg ccaacctgaa tccggaacgc actttcccgt cgctcttcga
     4561 gccagtccac ggttccgcgc cggatatcta cgggaaaaac attgctaacc ctatcgccac
     4621 gatttgggcc ggtgcaatga tgctcgattt tctcggcaat ggcgatgggc gtttccagca
     4681 agcgcataac ggtattctgg cagcaattga agaagtgatt gctcacgggc cgaaaacacc
     4741 tgatatgaaa ggcaatgcca ccacgccaca ggttgccgac gcgatttgca aaattatttt
     4801 gcgttaaggt caaaccagtt tatttgaacc gcgtcactga cgcggttttt ttattcgttc
     4861 tttgcagtaa ataacctgcg tcatttcacc ttttattgtt tccgtttcgt gttttatggc
     4921 tttccgtatt cttaattgtt taatttatgt aacatgcaaa taatcaattt gttaaacaca
     4981 acatacaact cattaccctg caaataatta caactggcaa aagccagaga cgggctttta
     5041 tgcccaggag atgactcatg atgagcaatg ttaagaaaaa agatgtgccg ctgataagca
     5101 tcagcctggt ggccattctt ttcatcgcag ctgcattaag ccttttccca caacaatcgg
     5161 ccgacgcggc caacgcaata tacacttttg ttacgcgtac gttaggttcc gccgtacagg
     5221 tattggtttt gctggcaatg ggactggtga tttatttagc caccagtaaa tacggcaata
     5281 ttcgtcttgg cgaaggaaaa ccggaataca gcacgctctc ctggctgttt atgtttattt
     5341 gtgccggttt aggttcttct acgctttatt ggggggttgc tgaatgggcc tattattatc
     5401 aaacgcctgg attaaatatc gcaccgcgtt cacaacaggc actcgaattt agcgtccctt
     5461 actccttctt ccactggggc atcagcgcct gggcaactta tacgctggcc tcattaatca
     5521 tggcttatca ctttcatgtg cgtaaaaaca aaggtctgag tctttccggc attattgccg
     5581 cgattaccgg cgttcgcccg caaggcccat ggggaaaact ggttgatttg atgttcctga
     5641 tcgccactgt cggcgcactg actatttccc ttgttgtcac cgcagcaacc tttactcgtg
     5701 gactttccgc gctgaccggt ttacccgata acttcaccgt gcaggcattt gtgatcctgc
     5761 tttccggcgg cattttttgc ctaagctcgt ggattggtat caacaacggt ttgcaacgtc
     5821 tgagcaaaat ggttggctgg ggcgcgttcc tgctgccatt actggtgctg attgtcggcc
     5881 caaccgaatt tattaccaac agcatcatca atgccatcgg tctgaccacg caaaacttcc
     5941 tgcaaatgag cttattcacc gatccgcttg gcgatggttc atttacccgc aactggaccg
     6001 ttttctactg gctgtggtgg atctcataca cccctggcgt agcaatgttt gtcacccgcg
     6061 tttcccgcgg tcgtaagatt aaagaagtta tctggggact gatcctcggc agcaccgtcg
     6121 gttgctggtt cttcttcggc gtaatggaaa gctatgccat tcatcagttt atcaatggcg
     6181 taatcaacgt cccacaggtg ctggaaacac tgggcggcga aacagctgta cagcaagttc
     6241 tgatgtcgtt gccagccggt aaattgttcc tcgccgcata cctgggcgtg atgattattt
     6301 tccttgcctc gcatatggat gcggtggcct acaccatggc tgcgaccagt acgcgtaatc
     6361 tccaggaagg tgacgatcct gaccgtgggc tgcgtctttt ctggtgcgtg gtgatcactc
     6421 tgatcccgct ttccatcttg tttaccggtg cttcgctgga aacgatgaaa accaccgtcg
     6481 tgctcacagc ccttcccttc ctcgtcattt tactggtgaa agtcggcgga tttattcgct
     6541 ggctgaaaca ggattacgcc gacattccgg ctcatcaagt tgaacattat ctcccgcaga
     6601 caccggttga agccctggaa aaaacaccag tgctccctgc gggaaccgta ttcaaaggcg
     6661 acaactgagc gccatcatcc taacgataaa ggtatcccta tgagcaatct gagccctgac
     6721 tttgtactac ccgaaaattt ttgcgctaac ccgcaagagg cgtggaccat tcctgcccgt
     6781 ttttataccg atcagaacgc gtttgaacac gaaaaagaga acgtcttcgc caaaagctgg
     6841 atttgcgtcg ctcacagcag cgaactggcg aatgccaatg attatgtgac gcgtgagatc
     6901 attggcgaaa gcatcgtgct ggtacgcggt cgtgataagg ttttgcgcgc gttctataac
     6961 gtgtgtccgc accgtggtca tcagttgttg agcggtggag gaaaagcaaa aaatgtgatt
     7021 acctgcccgt atcacgcatg ggcattcaaa ctcgatggca atctggccca tgcacgtaac
     7081 tgcgaaaacg tcgccaattt cgatagcgac aaagcgcaac tggttccggt gcgtctggaa
     7141 gaatatgccg gattcgtctt catcaacatg gaccccaacg ccaccagcgt agaagatcag
     7201 ttacccggtc tgggcgcgaa agtgctggaa gcctgcccgg aagtccacga tctgaaactg
     7261 gcggcccgct ttaccacccg cacgcctgcc aactggaaga acattgtcga taactatctc
     7321 gagtgctatc actgtggtcc ggcgcatcca ggtttctccg actccgtaca ggttgatcgt
     7381 tactggcaca ccatgcacgg taactggacg ctgcaatacg gtttcgccaa accgtccgaa
     7441 cagtcgttta aatttgaaga gggtacggat gcggcattcc acggtttctg gctgtggccg
     7501 tgcacgatgc tgaacgtcac cccgatcaaa gggatgatga cggtcattta tgaattcccg
     7561 gtggattctg aaactaccct gcaaaactac gatatttact tcaccaatga agagttaacc
     7621 gacgagcaaa aatcgctgat tgagtggtat cgcgatgtgt tccgtccgga agatttacgt
     7681 ctggttgaaa gcgtacagaa agggctgaaa tcgcgtggct atcgtggtca ggggcgcatc
     7741 atggccgaca gtagcggtag tggcatttcc gaacatggta tcgcccattt ccataatctg
     7801 ctggcgcagg tgtttaagga ctaatgacat cggcggcggt attttccgcc gctgggctga
     7861 tttttgatgg agtacagcaa tgtcagacta tcaaatgttt gaagtacagg tgagtcaggt
     7921 ggaaccgctt accgaacagg tgaaacgctt cacgctggtg gcaaccgatg gcaaaccgct
     7981 gcctgcgttc accggaggaa gtcacatcat tgtgcagatg agcgatggtg ataaccatta
     8041 cagcaatgcg tattcactac tgagttcgcc gcatgacacc tcttgttatc agattgccgt
     8101 tcggctggag gaaaactcgc gcggcggttc ccgctttttg catcagcagg taaaagtggg
     8161 cgatcggtta acgatttcaa cgcctaataa cctgtttgcg ctaattccct cagccagaaa
     8221 gcatctgttt atcgcgggcg gtattggtat cacccctttc ctgtcgcaca tggcagagct
     8281 gcaacacagc gacgtcgact ggcagctaca ttactgctcg cgaaatccag aaagttgcgc
     8341 atttcgtgat gagctagtcc agcatccgca ggctgagaaa gttcacttgc atcactcatc
     8401 aaccggaaca cgactggaat tagcgcgatt attggcggat atcgaacctg gcacacacgt
     8461 ttatacctgt ggtcccgagg cgctaaatga agcggtaaga agtgaagctg cgcgtctgga
     8521 catcgtcgcc gatacgctgc actttgagca atttgctatc gaagacaaaa ccggcgatgc
     8581 ctttaccctg gtgcttgccc gttccggaaa agagtttgtg gtgccggaag agatgactat
     8641 tttgcaggtt attgaaaata ataaagccgc gaaagtggaa tgtttatgtc gtgaaggggt
     8701 atgcggaacc tgcgaaacag caatactgga aggtgaagct gaccatcggg atcagtattt
     8761 tagcgatgaa gagcgtgcca gccagcaaag tatgttgatc tgttgttcgc gtgcgaaggg
     8821 taaacgcctg gtgttggatt tgtagtttgc caaagccgga tgtggcgctg agcgcgccca
     8881 gtccggcttc ggaagatttt actgcggata ttcctgcaat aaattgtgta atgcttccgc
     8941 catcagctca ccacgccagc cggaaatcag ctccggcaaa ttgttctgcg gtttcagttt
     9001 ccagtgccag ttcagcagtt ggttgatttg ccgacgcgat gccagcaatt cggcgctgat
     9061 cttatgcgtt tcgctcacgt cagtaatcag cgacttaatc gctttaaaca ctttacgata
     9121 accaggcatg tccatcaggt taagcatcgg ctgcggtaag gcctcttccg gcaatgtctg
     9181 cgctttttcc accagcgcca gcagcgtttt accgtgaaag cggatttcgc taccggataa
     9241 acccaggctg tccagttcgc ctaaacttcc cggcatataa cgcgcgaccg accacaaatg
     9301 ctcttcacgc acgacaaagt tcaccgccag atcacgctct cgcgccttgc gcagtcgcca
     9361 gtcggctaac agttgcagac aggccagttg gcgtgtgcgt aactgccagg cattggtgat
     9421 atcacgccag gcatcttccg gcgcaacgac ttcctgacga cgcatttgca tcaggcggca
     9481 ttcatccagc gccgcaggta gccagccgga ggcctccgtt tctaccataa gcttggcggt
     9541 gatcggtaac agataccaga catccgccgc tgcgtattca cactgacgtt cggtcagcgg
     9601 tctggccagc cagtcggtgc gcgattcact cttgtccagc gtaacgccgg aatactcttc
     9661 caccatggaa gcgaaacccc atgacatcgg gcgtccgcag aaggcagcaa ggatttgcgt
     9721 gtcaatcaag ggttgtggta attcgccaaa gacattgagg aacacttcca gatcttcact
     9781 gcctgcatgg agaaattttg tgatggacgg atcgcgcagg atcgctttca gcggtgacca
     9841 gtcggtgatc ccgagaggat caattagcgc cagatgctcg ccatcgaaaa gttgaatcaa
     9901 ccccagctgc gggtaataag tgcgcgtacg aacaaattca gtatccaggg ctatcgccgg
     9961 aaaggcacgg acggcttcac acaaagaagc cagcgcatcg tccgtggtaa tcatttggta
    10021 attcaaattg ttttctcttt agtgggcgtc aaaaaaaacg ccggattaac cggcgtctga
    10081 cgactgactt aacactcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
    10141 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
    10201 cggtactttg tagcccgtga gctgacggcg gcaaaaggtc accagtgact cttcggtaag
    10261 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
    10321 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
    10381 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
    10441 gaacccttct tcatccatca ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
    10501 ctcatctgta gcatccggac gctgccagta acccagcatc acctgcggtc ctttgacaca
    10561 aagctcaccc ggttgacccg gtggtacttc attatcatca tcatccacca gtttggcttc
    10621 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
    10681 gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact gtcctgtcag
    10741 tttcacccaa cgctctgcca ccacttgctg cactggcatc cctccgcctg cggaaagatg
    10801 cagactggag aaatccagct gctggaactc tttattgttc agcaacgcat tgaacaaggt
    10861 gttaacgccc gtgatagcgg taaacggata tttcgctaac tcttttacca accctggaat
    10921 atcgcgcggg ttagtgataa gcaggttctg cccacccagt tcgataaaca gcaggcagtt
    10981 aatggtcagg gcaaaaatgt gatacagcgg cagcgccgtc accaccagct ctttgcccgg
    11041 atgcaacagc ggaccatagg tcgcgttaac ctgttccagg ttcgccagca tattgcggtg
    11101 agtcagcatc gcgcctttcg ccacaccagt ggtgccgccg gtgtattgca gaaaagctaa
    11161 atcttccggc accagttcgg gtttgacgta ctgcatccgg tagccgttat gcagtgcgct
    11221 acgaaatgaa atggcatctg gcagatggta tttcggcacc aaacgcttga tgtatttaac
    11281 aacgaaattg actaccgtgc cttttgccgt agatagctga tcgcccatac gggtcagaat
    11341 tacgtgctga acggcggttt tatcaaccac tttttccagt gtgtgagcaa agttagacac
    11401 gataacaatc gccgatgcgc cgctatcgtt aagctgatgc tcaagctcac gcggggtata
    11461 caacgggtta acgtttacga cgatcatccc ggcacgcaaa atgccaaaca gcgccaccgg
    11521 atattgcaat aaattaggca tcatcaacgc aacgcgatcg cctttcttca gccccaaccc
    11581 ttgttgcaaa taagcggcaa acgcgcgact gcgttcttcc agcttgcgga aggtcattac
    11641 ctcccccata ttcacaaacg caggttgatc ggcgtagcgc gcgaccgact gctcaaacat
    11701 atctaccaga gattgataac ggtcagggtt gatctccgtc ggaacgtccg cgggataacg
    11761 gttaagccaa accttcttca attcttcacc tctaaaatgc gtgttcgtcg tcatcgcaac
    11821 cccaaatgat atacatgccg ttaacataat attaactcat cataccagct tgataattac
    11881 ccaacgaaaa ggttgcgaag cgcgtcacta tttattttta tctttaccgt aagaatgcag
    11941 aaacagcgga ccagccgctg tttctttttc tttaaaaaca agcaattatt cagttacaac
    12001 tgtttgtact ctcgcggggc cgggattata ccagccccat ccgccgtagc catagggcca
    12061 gccacgaccg ccataaaacc atggatcaat cggctgaggc ggcataatca cctgctgggt
    12121 taaatgccaa cgtttgtaac ccgttacttg catcaccata aatttatagg gcgtattgcc
    12181 gattttgccg tcaaccgcac cagtgattgg cccgactacc gtaacaagtt gtccacggaa
    12241 atccaccggg tccagaaaac cgttcacatc ggcataaatg cgaccgcgag aaggttctcc
    12301 cagcgtcggt ctggctccgc tgtccagcgg tacggtagca atttccaggc gggttttccc
    12361 ttgctggttt tgtaccgcaa ccactttgcc accaaagcgt gcctcctgac caacgtacag
    12421 ctgcggcgca ctcatcaccc gaactaaatc ttgttgcggc gtgggactgc tgcctttaat
    12481 ggcgtccggc acagtgacac aaccgctcag cattagcgca aacgtacctg ccagtatgcc
    12541 tttgataaca ttcttttgaa ccgccatgat gcgactcctt tttttctcag ggcatactct
    12601 taagattcat tctttgcccg gaagtttctt ccatgcgacg ttgttacgta aataaaccgg
    12661 ttcggcatgt tccaccgcca ccgttttacc ctcggcaaac atctgacacg caatcggcag
    12721 catatcttcc gccgctggca gtaaaacttc tccgtcgcgc aaaaccagcc cgctctcttt
    12781 accgagatcc ggccaggctt gccagcccgt ccctaccgtt acccattcgc cggaaagctg
    12841 ttgcattcgt tcatgaacga gttcgggttt gagtacggct tcggtttctt caccgcgcca
    12901 gataccgttt tcatcacgct gatattcggc ccagtaaact tcgcccattc gcgcgtcaat
    12961 ggctgccagc acgcgggttg cgccgttttt gcgccacgcc ccttgcgcca tcgtcattag
    13021 cgtggagacg ccaatcatcg gtagctctgc gccaagtgcc agcccttgcg caatgccaat
    13081 accaatgcgc acgccggtaa agctaccggg gccgcgcccg taagccagag cgttaatatc
    13141 agtcagggaa gttccgctgg tggtcaggat atcctgcacc atcggtaaga ttcgttgagt
    13201 atgttcacga gggcaaagct caaaatgagc gttgacagta ccgtcgttcc acagggcgac
    13261 agagcaggcc tctgtcgcgg tatcgatagc cagaattcgc atgggtcttc gtgcttagat
    13321 caataaaaag gcgcgcatca taccatactc cgtaacaaat tacctggagg atggtatcgc
    13381 aaggaaacga accgcacggg caatgtcacg agtgcgtggc gcgggcggca gactggcgag
    13441 aaacgtcgcg ccgtaaggac gcatcaccag ccgattgtca caaatcacca gcacgccacg
    13501 atcgtcggcg tcgcgaatca gtcgccctac cccctgtttg agagtaatga cggcatctgg
    13561 tagttgcact tcatcgaacg ggtcgccacc gcgcaaacga caatcttcca tgcgcgcttt
    13621 taacagtgga tcatccggcg aggtaaacgg caacttgtcg ataatcacca atgacaatgt
    13681 atcgccacgc acgtccaccc cttcccagaa actactggtt gccacaagaa gcgcgttacc
    13741 ggcgctgaca aattgctgca acagttgccc tttgctggtt tccccctgca acaaaacggg
    13801 aagcgtcatg gtagcgcgga actgctcggc cagatcacgc atcatggcgt gcgaggtaca
    13861 aagcataaaa caacgaccgt tgttagcttc gatgatcggt cgcagcattg ccgctaactg
    13921 gcgagcagaa cctggctggt tggtttgcgg cagattgcgc ggcacacaga gtaacgcctg
    13981 gcggctgtaa tcaaacgggc ttggcaatag caacgactcc gcctgttcta tcccaagccg
    14041 cgaggtgaaa tgatgcagat cgtcgttcac cgacagcgtt gctgaggtga agatccagct
    14101 accaggcttt tgcgccatta actctttgaa tttatccgcc acgctgagcg gcgtgagagc
    14161 cagagtaaaa tggcgcgaag tgcattcgta ccagtagctg tagcccggct gattgatctc
    14221 ttttagccgc ttcagccgtg tgcgatacaa cgtagcgcgc tcaaatgccg catccagcaa
    14281 ggcggaacgc cccagtgaca gtttcgccac gtcataacaa agttccaggg tgtcatcgag
    14341 cagtaaaaat gcccgctgaa tttgcggatt agctaacagc tcacgcaggt taccgcgata
    14401 acctggctca ccgagttgca gacgaaaatc ctgcgcactc tgggcaagac gatcagcgca
    14461 cttttgtaac tgctgggtgt cttttaattc ggtgcggtag gcgatggtga tgtcttttgc
    14521 caggtcgagc agttgtcgac tggagagtga ctgaccaaaa tactggctgg caatgtccgg
    14581 tagctggtgg gcttcgtcga agatcatgac gtccgcttcc gggatcagtt cgccaaatcc
    14641 actctcttta accaccatat ccgccagaaa gagatgatgg tttaccacca ccacatcggc
    14701 gtccatcgct tttttacgtg ctttgaccac aaagcaatct ttatacatcg ggcagtcgct
    14761 gccaagacag ttgtcgttgg tgctggtgac cagcggccac gcctgtgaat cttccgccac
    14821 gctgacgcag gtgctgatat caccatcgac tgtttgatta gaccaggagc gcagcaggat
    14881 cacatcgctt aagatttgta ccggcagatc gccccccgcc agcgcctgct gttcgagacg
    14941 ttcgaggcag aggtagtttg agcgcccttt cagcagcgcc acgttgcccg tatatttcaa
    15001 tgcctttgag actgttggca aatcgcggct gtagagctga tcctgcaacg cttttgagcc
    15061 ggtcgagata atgactttct ttttcgcccg cagcgcagga gccaggtaag cgtaggtttt
    15121 gcccgtaccg gttcctgctt ccaccaccag cggctggcct ttttctatcg cctgggtgac
    15181 ggctaccgcc atctgtcgct gtggttctcg cggcttaaag cctggtatcg ctttcgccag
    15241 ctgaccgtct ggtgcaaaat cgtccgtcac actaccccct gttgatttga acagggatta
    15301 tgtcaggatg agggcgcttt cgccagttga agtggtgacg gcaacctcac attgtggcag
    15361 tctttgcagc acgaaaatgg aagtataatg aggacaaaat gatgactatc gttcgtatcg
    15421 atgctgaagc ccgctggtct gatgtagtaa tccacaacaa cacgctctac tacactggtg
    15481 taccggaaaa cctcgacgcc gatgcttttg agcaaaccgc caacaagctg gcgcagattg
    15541 acgccgtgct ggaaaaacat ggcagcaata aatcgagcat tctggatgcc accattttcc
    15601 tggctgataa aaacgacttc gcggcgatga ataaagcgtg ggatgcctgg gttgtggcgg
    15661 gtcatgcgcc ggtgcgctgc acggtgcaag cggggctgat gaacccgaag tataaagttg
    15721 aaattaagat tgtggctgcg gtgtaagctt tatcgaagca aaataagtca gacgataatt
    15781 tatcgataat actggtcggt tttacataaa tcgaccagag attaagatat tactgttgat
    15841 gctcctgcta attcattatt tatttttata atttcaattt tagctataaa gctaactata
    15901 taaataaaca taaccatgcc gttgtgttcg cccttcgatt agcacctctg gttttctttc
    15961 ttagctgaac aatctggtta tagcgtgcgg actgaccgtt aattttctcc atcttactgg
    16021 ataacacacc atgagctgct acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc
    16081 atatcgttat aatccctttg atattagatg caaattaagg tcatatatag ccttatcgat
    16141 aacatggtta attttaagga taagaatatg cctgctgtaa tagataaagc cctggatttc
    16201 attggtgcca tggatgtatc agcgccaaca ccaagttcga tgaatgaaag cacagcgaag
    16261 ggaatcttta aatatttaaa agaactggga gtacccgcga gtgccgctga tattacggcg
    16321 cgagctgacc aggaaggctg gaaccctggg ttcacggaaa aaatggttgg atgggcaaaa
    16381 aaaatggagt ctggtgaacg cactgtaatt aaaaatcctg aatacttttc aacatatatg
    16441 caggaagaac taaaagcact ggtctgagtt aaatttatat cagcataaat gggtcaggac
    16501 gcttttaatc acatataaaa agcgtccttt ttctcacaat caaatgatca tcggcttcta
    16561 taacgaaatc gatacggatt agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt
    16621 cgctcgccgg agtggttggc acgcagctct tctgccacca gcgcgattgc ctggccgctg
    16681 ctcatccctt gtgccatcag ttcctggatc cgctcgacag ctttttgctg ctgttcatgg
    16741 gtgagtgaag gtaaacctgc aaacattgtt aactcctgct aaattgttgg cgctaattat
    16801 ttcatgctac ccggcacata gccagtagag tcaggactga tgaagacgtt atctcccgct
    16861 gtgattactt taccctggcg tcaggacgcc gctgaatttt atttctcccg cttaagccac
    16921 ctgccgtggg cgatgctttt acactccggc tatgccgatc atccgtatag ccgctttgat
    16981 attgtggtcg ccgatccgat ttgcacttta accactttcg gtaaagaaac cgttgttagt
    17041 gaaagcgaaa aacgcacaac gaccactgat gacccgctac aggtgctcca gcaggtgctg
    17101 gatcgcgcag acattcgccc aacgcataac gaagatttgc catttcaggg cggcgcactg
    17161 gggttgtttg gctacgatct gggccgccgt tttgagtcac tgccagaaat tgcggaacaa
    17221 gatatcgttc tgccggatat ggcagtgggt atctacgatt gggcgctcat tgtcgaccac
    17281 cagcgtcata cagtttcttt gctgagtcat aatgatgtca atgcccgtcg ggcctggctg
    17341 gaaagccagc aattctcgcc gcaggaagat ttcacgctca cttccgactg gcaatccaat
    17401 atgacccgcg agcagtacgg cgaaaaattt cgccaggtac aggaatatct gcacagcggt
    17461 gattgctatc aggtgaatct cgcccagcgt tttcatgcga cctattctgg cgatgaatgg
    17521 caggcattcc ttcagcttaa tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt
    17581 gaacagggtg caattttaag cctttcgcca gagcggttta ttctttgtga taatagtgaa
    17641 atccagaccc gcccgattaa aggcacgcta ccacgcctgc ccgatcctca ggaagatagc
    17701 aaacaagcag aaaaactggc gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc
    17761 gatttaatgc gtaatgatat cggtcgtgtt gccgtagccg gttcggtaaa agtaccagag
    17821 ctcttcgtgg tggaaccctt ccctgccgtg catcatctgg tcagcactat aacggcgcga
    17881 ctaccagaac agttacacgc cagcgatctg ctgcgcgcag cttttcctgg tggctcaata
    17941 accggggctc cgaaagtacg ggctatggaa attatcgacg aactggaacc gcagcgacgt
    18001 aatgcctggt gcggcagcat tggctatttg agcttttgcg gcaacatgga taccagcatt
    18061 actatccgca cgctgactgc cattaacgga caaatatact gctctgcggg cggtggaatt
    18121 gtcgccgata gccaggaaga agcggaatat caggaaactt ttgataaagt taataagata
    18181 ttacgccaac tggagaagta agacgtggaa taccgtagcc tgacgcttga ttatttttta
    18241 tctcgctttc aacttttgcg cccgcaaatt aaccgggaaa ccctaaatca tcgtcaggct
    18301 gccgtgttaa tccccatcgt ccgtcgaccg caaccggggt tgttgctgac tcagcgttca
    18361 attcatctgc gtaaacacgc tggacaagtg gcattccctg gaggtgcagt cgatgacacg
    18421 gacgcatcag ttatcgccgc cgcgctgcgc gaagctgaag aagaggtcgc tataccgcct
    18481 tccgccgttg aagttatcgg cgtgctgccg cccgtcgata gcgtaactgg ctatcaggta
    18541 accccagtgg tcggcattat cccgcccgat ctgccgtatc gcgccagtga agatgaagtc
    18601 tcggcggtgt ttgaaatgcc gctcgcccag gcattacatc tgggtcgtta tcacccttta
    18661 gatatctacc gccgtggcga ttcacatcgg gtatggctgt cctggtacga acagtatttt
    18721 gtatggggaa tgaccgcagg cataattcgt gagctggcgc tgcaaattgg tgtgaaaccc
    18781 tgactatact tatctttaca tctacaaaac actacttgag acaatcatcg caatattagt
    18841 taaatcgcgg tttttgatta gtttaattca tgtgaatagt taagccagtc gccgcgttcc
    18901 ctcttacact atgcgctgtt attagttcgt tactggaagt ccagtcacct tgtcaggagt
    18961 attatcgtga ttagtctatt cgacatgttt aaggtgggga ttggtccctc atcttcccat
    19021 accgtagggc ctatgaaggc gggtaaacag ttcgtcgatg atctggtcga aaaaggctta
    19081 ctggatagcg ttactcgcgt tgccgtggac gtttatggtt cactgtcgct gacgggtaaa
    19141 ggccaccaca ccgatatcgc cattattatg ggtcttgcag gtaacgaacc tgccaccgtg
    19201 gatatcgaca gtattcccgg ttttattcgc gacgtagaag agcgcgaacg tctgctgctg
    19261 gcacagggac ggcatgaagt ggatttcccg cgcgacaacg ggatgcgttt tcataacggc
    19321 aacctgccgc tgcatgaaaa cggtatgcaa atccacgcct ataacggcga tgaagtcgtc
    19381 tacagcaaaa cttattattc catcggcggc ggttttatcg tcgatgaaga acactttggt
    19441 caggatgctg ccaacgaagt gagcgtgccg tatccgttca aatctgccac cgaactgctc
    19501 gcgtactgta atgaaaccgg ctattcgctg tctggtctcg ctatgcagaa cgaactggcg
    19561 ctgcacagca agaaagagat cgacgagtat ttcgcgcatg tctggcaaac catgcaggca
    19621 tgtatcgatc gcgggatgaa caccgaaggt gtactgccag gcccgctgcg cgtgccacgt
    19681 cgtgcgtctg ccctgcgccg gatgttagtt tccagcgata aactgtctaa cgatccgatg
    19741 aatgtcattg actgggtaaa catgtttgcg ctggcagtta acgaagaaaa cgccgccggt
    19801 ggtcgtgtgg taactgcgcc aaccaacggt gcctgcggta tcgttccggc agtgctggct
    19861 tactatgacc actttattga atcggtcagc ccggacatct atacccgtta ctttatggca
    19921 gcgggcgcga ttggtgcatt gtataaaatg aacgcctcta tttccggtgc ggaagttggt
    19981 tgccagggcg aagtgggtgt tgcctgttca atggctgctg cgggtcttgc agaactgctg
    20041 ggcggtagcc cggaacaggt ttgcgtggcg gcggaaattg gcatggaaca caaccttggt
    20101 ttaacctgcg acccggttgc agggcaggtt caggtgccgt gcattgagcg taatgccatt
    20161 gcctctgtga aggcgattaa cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc
    20221 gtctcgctgg ataaggtcat cgaaacgatg tacgaaaccg gtaaggacat gaacgccaaa
    20281 taccgcgaaa cctcacgcgg tggtctggca atcaaagtcc agtgtgacta atacttctta
    20341 ctcgcccatc tgcaacggat gggcgaattt atacccgctt tctcgtctgc tgtaatattc
    20401 cccactacac ttccactgtt gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg
    20461 catgcaaaaa gcacaacgga tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac
    20521 gatttgcgca ctcgttacgc tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa
    20581 cttaaatcaa caacgggtag tacaattcgc caatcacgct gtagaggaat tagataaagt
    20641 actgcttccc ctacaggcag gtagcgaagt cttgcttccg ctgattggtc tgccctgctc
    20701 tgtcgcccat ttgccattac gtaaacaggc ggcaaaactc caaactgtgc gatccattgg
    20761 cctggtgcaa gacggcacac tctattgctc cagcattttt ggttatcgca atgtgcccgt
    20821 cgtggacatt ctggctgaac ttcctgcacc gcaaccactt ttacgcctga cgatcgaccg
    20881 tgccctgatt aaaggcagtc cggttttgat tcaatggacg cctgcagcgg gcagtagcaa
    20941 tgctggggtc atggagatga ttaacatcga cttactgacg gcaatgctcc ttgagccaca
    21001 actgccgcaa atcagtagcg ccagcctgac ggtggacaaa cggcatttgc tctatggtaa
    21061 tgggctggta gattcccttc cgcaacctga agacaatgaa aactatcagg tttcttcgca
    21121 acgctttcct tttaccatta acgttaatgg tccgggggct acggcgctgg catggcacta
    21181 tcttccaaca caattaccgc tggcggtgct gctaagttta ctggtgggct acatcgcctg
    21241 gctggcgacc gcttaccgga tgagcttttc ccgcgaaatc aatctgggcc tggcgcaaca
    21301 tgagttcgaa ttgttctgtc agcctttgct taatgcgcgc agccagcaat gtattggtgt
    21361 agagattttg ctgcgctgga acaatccgcg tcagggctgg atttcaccgg atgtgtttat
    21421 tcctatcgcg gaagaacatc atttaattgt gccactgacc cgctatgtga tggcagaaac
    21481 cattcgtcag cgccatgttt tcccgatgag tagtcagttt catgttggca ttaacgtcgc
    21541 acccagccat tttcgccgtg gtgtactgat aaaagatctc aatcagtact ggtttagcgc
    21601 tcacccgatt cagcaactaa tcctcgaaat caccgaacgc gatgccttac tggatgttga
    21661 ttatcggatt gcccgcgagc tacatcgtaa caacgtcaaa ctggcgattg atgacttcgg
    21721 caccggcaac agttcatttt cctggcttga aacattacgt cctgacgtgc tgaaaattga
    21781 taagtcattt accgcagcta taggttctga cgcggttaac tcgacggtga ccgatatcat
    21841 catcgcgctg gggcaaagac tgaatattga actggtggcg gagggcgtgg aaacgcaaga
    21901 acaggcgaag tatttgcgcc gtcatggcgt gcatattttg caagggtatt tgtacgcaca
    21961 gccgatgccg ctacgtgatt ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg
    22021 gcataatgga catatcacgc ccattatgcc gttacgttaa cgcttactca tcttcatcgt
    22081 gcgccggttg ctctttaaca atgcgaacca gatcaacacg ataatcattg gcttcaatga
    22141 tggtgatatg cagtggccct acatcaatca catcgcccac acggggaatg tgaccatttg
    22201 ccgagatcac caggcccgcg accgtcgcga tatcatcgtc atcggcaagg tgctcaacat
    22261 caagcgcctg ctgcaaggca tgcaaatctg taccgccttt taccagccag ccgtcaccat
    22321 cggtaatgat ttccggcgtt tcgtcagcgt ccgggaattc acccgcaatg gcttccagca
    22381 catccagcgg cgtgaccaag ccttgtacca caccaaactc gttggtcacg ataacaaagc
    22441 tcccgcgagc gcgacgcagc acgcccagca ggttgatcgg atcgagggtt tccgggacga
    22501 taatcgccgg agacgccgaa gcaatcgccg ccacatcaac gccctcttcc agcgccacca
    22561 gcagttcttt agcacgtacg attccgatga tttcatccag ttcaccgcga cataccggga
    22621 acaggctgtg cggtgaggag agcagctgct cgcggatttc atcgaccccg agattagcgt
    22681 caacccagct tatttcaccg cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg
    22741 tcagtacgcc gttaatcatg taacgttctt cttcggcaaa tgcaccttcc gggatcggca
    22801 tcggcatcgg gttatcggca tcgtgctgaa cattggcctg acgtttcccg cccatcaaac
    22861 gcaggatggc atcggcagta cgcgctcgca gcggcaaagt cgactggtga cgaataaagt
    22921 tgcgacgcgc aatctggtta aacacttcga tgatgatcga gaagccaatc gcggcataca
    22981 ggtaaccttt cggaatgtgg aaaccgaaac cttctgccac cagactcaga ccaatcatta
    23041 acaggaagct cagacagagc accaccaccg tggggtgctg gttaacgaat cgcgtcagcg
    23101 gtttggatgc cagcaacata accgccatcg caatcactac agccgccatc atcaccgaca
    23161 gatggttaac catccctact gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga
    23221 tgacaatctg tgtgacgacc acccagaaac tggcgtagcc tttaccgtgg ccggaatcat
    23281 gatcgcggtt ttccagccgt tcatgcagtt cggttgttgc tttgaacagc aagaatatcc
    23341 ccccgaacaa cataatcagg tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg
    23401 gtttggtcag cgtgaccatc catgaaatca gcgacagcag ccccagacgc ataatcagcg
    23461 ccagtgataa ccccagcaaa cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa
    23521 gaatggcgat gaagaccagg ttatcgatac ccagcacaat ttcgagaaca acaagcgtga
    23581 gtagccccgc ccaaattgag gggtccatta agaattccat gacaagctcc tgcttaagga
    23641 atagctattc gacgccagaa ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg
    23701 gcatagagag ccagaaaggc aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg
    23761 atccatactg cgggctactg ccctatactc catggttgtt aaacgggagt taaacatatc
    23821 agagacgcct ctgatttggc aaagatttac cttcctttgc aaacgaatgt gacaaggata
    23881 ttttaccttt cgaaatttct gctaatcgaa agttaaatta cggatcttca tcacataaaa
    23941 taattttttc gatatctaaa ataaatcgcg aaacgcaggg gtttttggtt gtagccctta
    24001 tctgaatcga ttcgattgtg gacgacgatt caaaaataca tctggcacgt tgaggtgtta
    24061 acgataataa aggaggtagc aagtgaccat tgctattgtt ataggcacac atggttgggc
    24121 tgcagagcag ttgcttaaaa cggcagaaat gctgttaggc gagcaggaaa acgtcggctg
    24181 gatcgatttc gttccaggtg aaaatgccga aacgctgatt gaaaagtaca acgctcagtt
    24241 ggcaaaactc gacaccacta aaggcgtgct gtttctcgtt gatacatggg gaggcagccc
    24301 gttcaatgct gccagccgca ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt
    24361 taacattcca atgctcgtgg aaacgttaat ggcccgtgat gatgacccaa gctttgatga
    24421 actggtggca ctggcagtag aaacaggccg tgaaggcgtg aaagctctga aagccaaacc
    24481 ggttgaaaaa gccgcgccag cacccgctgc cgcagcacca aaagcggctc caactccggc
    24541 aaaaccaatg gggccaaacg actacatggt tattggcctt gcgcgtatcg acgaccgtct
    24601 gattcacggt caggtcgcca cccgctggac caaagaaacc aatgtctccc gtattattgt
    24661 tgttagtgat gaagtggctg cggataccgt tcgtaagaca ctgctcaccc aggttgcacc
    24721 tccgggcgta acagcacacg tagttgatgt tgccaaaatg attcgcgtct acaacaaccc
    24781 gaaatatgct ggcgaacgcg taatgctgtt atttaccaac ccaacagatg tagagcgtct
    24841 cgttgaaggc ggcgtgaaaa tcacctctgt taacgtcggt ggtatggcat tccgtcaggg
    24901 taaaacccag gtgaataacg cggtttcggt tgatgaaaaa gatatcgagg cgttcaagaa
    24961 actgaatgcg cgcggtattg agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa
    25021 aatgatggat ctgatcagca aaatcgataa gtaacgtatt gtgttgatta tcactcagtt
    25081 ttcacactta agtcttacgt aaacaggaga agtacaatgg agattaccac tcttcaaatt
    25141 gtgctggtat ttatcgtagc ctgtatcgca ggtatgggat caatcctcga tgaatttcag
    25201 tttcaccgtc cgctaatcgc gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc
    25261 ggtattatta tcggtggtac gctggaaatg atcgcgctgg gctggatgaa catcggtgct
    25321 gcagttgcgc ctgacgccgc tctggcttct atcatttcta ccattctggt tatcgcaggt
    25381 catcagagca ttggtgcagg tatcgcactg gcaatccctc tggccgctgc gggccaggta
    25441 ctgaccatca tcgttcgtac tattaccgtt gctttccagc acgctgcgga taaggctgct
    25501 gataacggca acctgacagc gatttcctgg atccacgttt cttctctgtt cctgcaagca
    25561 atgcgtgtgg ctattccggc cgtcatcgtt gcgctgtctg ttggtaccag cgaagtacag
    25621 aacatgctga atgcgattcc ggaagtggtg accaatggtc tgaatatcgc cggtggcatg
    25681 atcgtggtgg ttggttatgc gatggttatc aacatgatgc gtgctggcta cctgatgccg
    25741 ttcttctacc tcggcttcgt aaccgcagca ttcaccaact ttaacctggt tgctctgggt
    25801 gtgattggta ctgttatggc agtgctctac atccaactta gcccgaaata caaccgcgta
    25861 gccggtgcgc ctgctcaggc agctggtaac aacgatctcg ataacgaact ggactaacag
    25921 gtgagcgaaa tggttgatac aactcaaact accaccgaga aaaaactcac tcaaagtgat
    25981 attcgtggcg tcttcctgcg ttctaacctc ttccagggtt catggaactt cgaacgtatg
    26041 caggcactgg gtttctgctt ctctatggta ccggcaattc gtcgcctcta ccctgagaac
    26101 aacgaagctc gtaaacaagc tattcgccgt cacctggagt tctttaacac ccagccgttc
    26161 gtggctgcgc cgattctcgg cgtaaccctg gcgctggaag aacagcgtgc taatggcgca
    26221 gagatcgacg acggtgctat caacggtatc aaagtcggtt tgatggggcc gctggctggt
    26281 gtaggcgacc cgatcttctg gggaaccgta cgtccggtat ttgcagcact gggtgccggt
    26341 atcgcgatga gcggcagcct gttaggtccg ctgctgttct tcatcctgtt taacctggtg
    26401 cgtctggcaa cccgttacta cggcgtagcg tatggttact ccaaaggtat cgatatcgtt
    26461 aaagatatgg gtggtggctt cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg
    26521 tttgtcatgg gggcattggt taacaagtgg acacatgtca acatcccgct ggttgtctct
    26581 cgcattactg accagacggg caaagaacac gttactactg tccagactat tctggaccag
    26641 ttaatgccag gcctggtacc actgctgctg acctttgctt gtatgtggct actgcgcaaa
    26701 aaagttaacc cgctgtggat catcgttggc ttcttcgtca tcggtatcgc tggttacgct
    26761 tgcggcctgc tgggactgta agactgttgt acactaccgg ggccttttgg ccccgttttt
    26821 ttatctggag gattaatgac aatcacggac ctggtactga ttcttttcat cgccgcactc
    26881 ctggccttcg cgatctacga tcagttcatc atgccccgcc gtaacggccc caccctgctg
    26941 gcaattcctt tgctccggcg tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc
    27001 attcttatct ataacaacgt cacgaatcat ggtgcgttaa taacgacatg gttattaagc
    27061 gcactggctc tgatgggttt ttatatattc tggatccgca ttccgaagat catctttaaa
    27121 caaaaaggtt ttttcttcgc caatgtctgg attgaatata gccgaatcaa agcgatgaac
    27181 ttgtcggaag atggcgtgtt ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt
    27241 cgaaatatcg acgatctgga aaaaatttat aagcttctcg tttcaactca ataagttatg
    27301 aatttagcca aagctatgtt tagtgtatt
//
LOCUS       DAZGXZ010000064        26568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000064 DAZGXZ010000000
VERSION     DAZGXZ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.064"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..613)
                     /locus_tag="ACHKJF_002782"
     CDS             complement(<1..613)
                     /locus_tag="ACHKJF_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP0950115.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKK"
     gene            743..1684
                     /gene="rihB"
                     /locus_tag="ACHKJF_002783"
     CDS             743..1684
                     /gene="rihB"
                     /locus_tag="ACHKJF_002783"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP0950116.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            1784..3034
                     /gene="nupX"
                     /locus_tag="ACHKJF_002784"
     CDS             1784..3034
                     /gene="nupX"
                     /locus_tag="ACHKJF_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0950117.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAEFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWTDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(3090..4178)
                     /gene="yeiI"
                     /locus_tag="ACHKJF_002785"
     CDS             complement(3090..4178)
                     /gene="yeiI"
                     /locus_tag="ACHKJF_002785"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0950118.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDET
                     VLAINDTHLLEQLSPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLAGEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(4181..5038)
                     /gene="nfo"
                     /locus_tag="ACHKJF_002786"
     CDS             complement(4181..5038)
                     /gene="nfo"
                     /locus_tag="ACHKJF_002786"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP0950119.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(5112..6161)
                     /gene="yeiH"
                     /locus_tag="ACHKJF_002787"
     CDS             complement(5112..6161)
                     /gene="yeiH"
                     /locus_tag="ACHKJF_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP0950120.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            6260..7141
                     /gene="yieE"
                     /locus_tag="ACHKJF_002788"
     CDS             6260..7141
                     /gene="yieE"
                     /locus_tag="ACHKJF_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP0950121.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            7346..8815
                     /gene="lysP"
                     /locus_tag="ACHKJF_002789"
     CDS             7346..8815
                     /gene="lysP"
                     /locus_tag="ACHKJF_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP0950122.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            9109..11100
                     /gene="cirA"
                     /locus_tag="ACHKJF_002790"
     CDS             9109..11100
                     /gene="cirA"
                     /locus_tag="ACHKJF_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP0950123.1"
                     /translation="MFRLNPFVRGGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSFNYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(11132..11968)
                     /gene="yeiG"
                     /locus_tag="ACHKJF_002791"
     CDS             complement(11132..11968)
                     /gene="yeiG"
                     /locus_tag="ACHKJF_002791"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0950124.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            12226..12894
                     /gene="folE"
                     /locus_tag="ACHKJF_002792"
     CDS             12226..12894
                     /gene="folE"
                     /locus_tag="ACHKJF_002792"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP0950125.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            12911..14068
                     /gene="yeiB"
                     /locus_tag="ACHKJF_002793"
     CDS             12911..14068
                     /gene="yeiB"
                     /locus_tag="ACHKJF_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP0950126.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLVGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            14210..15250
                     /gene="galS"
                     /locus_tag="ACHKJF_002794"
     CDS             14210..15250
                     /gene="galS"
                     /locus_tag="ACHKJF_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP0950127.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSCHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            15530..16528
                     /gene="mglB"
                     /locus_tag="ACHKJF_002795"
     CDS             15530..16528
                     /gene="mglB"
                     /locus_tag="ACHKJF_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP0950128.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSISVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            16589..18109
                     /gene="mglA"
                     /locus_tag="ACHKJF_002796"
     CDS             16589..18109
                     /gene="mglA"
                     /locus_tag="ACHKJF_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP0950129.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            18125..19135
                     /gene="mglC"
                     /locus_tag="ACHKJF_002797"
     CDS             18125..19135
                     /gene="mglC"
                     /locus_tag="ACHKJF_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP0950130.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(19206..20441)
                     /gene="preA"
                     /locus_tag="ACHKJF_002798"
     CDS             complement(19206..20441)
                     /gene="preA"
                     /locus_tag="ACHKJF_002798"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP0950131.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(20435..21673)
                     /gene="preT"
                     /locus_tag="ACHKJF_002799"
     CDS             complement(20435..21673)
                     /gene="preT"
                     /locus_tag="ACHKJF_002799"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP0950132.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSNVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(21992..22231)
                     /gene="yeiS"
                     /locus_tag="ACHKJF_002800"
     CDS             complement(21992..22231)
                     /gene="yeiS"
                     /locus_tag="ACHKJF_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP0950133.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(22234..22953)
                     /gene="sanA"
                     /locus_tag="ACHKJF_002801"
     CDS             complement(22234..22953)
                     /gene="sanA"
                     /locus_tag="ACHKJF_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP0950134.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(23103..23987)
                     /gene="cdd"
                     /locus_tag="ACHKJF_002802"
     CDS             complement(23103..23987)
                     /gene="cdd"
                     /locus_tag="ACHKJF_002802"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP0950135.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(24117..24812)
                     /gene="yohK"
                     /locus_tag="ACHKJF_002803"
     CDS             complement(24117..24812)
                     /gene="yohK"
                     /locus_tag="ACHKJF_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP0950136.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(24809..25207)
                     /gene="yohJ"
                     /locus_tag="ACHKJF_002804"
     CDS             complement(24809..25207)
                     /gene="yohJ"
                     /locus_tag="ACHKJF_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP0950137.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            25446..26396
                     /gene="dusC"
                     /locus_tag="ACHKJF_002805"
     CDS             25446..26396
                     /gene="dusC"
                     /locus_tag="ACHKJF_002805"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP0950138.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKKYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         6527 a         6591 c         6897 g         6553 t
ORIGIN      
        1 ctttcttgct tttaattaat aaaccgtcgt gaatgaattg cgcgagaaca tataaaagat
       61 gtcgataaga aacgcctaaa tactctgccg cttgcgtatg cttttcgtga taaagatcac
      121 cttcttgcga gagtaatata aatgctgcca ggcgattcac taacggaaat gattgattct
      181 gagttaaaga aacaatatta cgataatttt tatgacttaa ggtgacgcaa agttttcgta
      241 aaaatagcgt gtcgtttaat aacagcggac ggtaatgttt catagggagc gcaaggcacc
      301 aacactcttc aatcgcctgt actgcacgtg gttcatggtc tttatcgatt aactcaatct
      361 cgccaataaa acagggggcg gcaaagaaat cgatcagcga cacgcgacca ttagctagcg
      421 tggcgtaaag cctggcgcgg cctcgcgtca ggtaaaacag ccaggacggc tgttgccctt
      481 cctgcacaat gtagtcacgc gctaaaaaat gaaacagccg cgtatcggct gaaacatccg
      541 ttaaaaagca atccttaaac gcggattcac tcatcaattg ctgcttaaga tcattattgt
      601 ggatttcttt cacgatgttt atccgctatg agatttctca tatttagcat acgtacgcgg
      661 ttggtaagat tcaatcatcg gcccggtcga attttctttg ctgtagaaaa aataacgcaa
      721 ctggaaacag aggaaataaa caatggaaaa gagaaaaatt attctggatt gtgatccggg
      781 tcatgatgat gctattgcaa tgatgatggc agcgaaacat ccggcaatag atttattagg
      841 catcactatt gtagcgggta atcagacgct tgataaaaca ttaattaatg gcctgaatgt
      901 ttgccagaaa ctggagatta atgttccggt ttatgcgggg atgccgcaac ccattatgcg
      961 taaacaaatc gttgccgata atattcacgg tgaaaccgga ctggatggcc cggtattcga
     1021 gccgctgacc cgccaggcag aaagtaccca cgcggtgaaa tacatcattg ataccctgat
     1081 ggcaagcgat ggcgatatca ctctggtgcc ggttggtccg ctttcaaata tcgcggtggc
     1141 aatgcgtatg caacccgcga tcctgcccaa aatccgtgaa attgtgctaa tgggcggcgc
     1201 ttacggtaca ggcaacttca cgccatctgc cgagttcaac atctttgctg acccggaagc
     1261 agcccgcgtg gtgttcacct ctggcgttcc attagtgatg atgggcctcg atcttaccaa
     1321 ccagaccgtt tgcaccccgg acgtgattgc tcggatggaa agggcaggcg gccctgccgg
     1381 agagctgttc agcgacatca tgaacttcac tctcaaaacg cagttcgaaa actacggcct
     1441 ggctggcggc ccggtgcacg acgccacctg catcggttat ctgattaacc ctgatggcat
     1501 taaaacccag gagatgtacg tcgaagtgga cgtcaacagt ggcccttgct atgggcgtac
     1561 cgtctgcgac gagctgggcg ttcttggcaa gcccgctaat accaaagtcg gcatcactat
     1621 tgataccgac tggttctggg gcttagtcga agagtgcgtg cgcggctaca tcaaaaccca
     1681 ttaagtgttg ataacaagcc gggtcagtat cgcaagatcc aagcccggct atttctgatg
     1741 ggcagaaagc cgtcgcccaa tatgacatga agagataata actatggatg tcatgagaag
     1801 tgttctggga atggtggtat tgctgacgat tgcgtttttg ctgtcagtaa acaagaagaa
     1861 gatcagcctg cgtaccgttg gcgcggcgtt agtgttacag gtcgtgattg gcgggattat
     1921 gctttggtta ccgccagggc gttgggtcgc tgaaaaagtc gcttttggcg tgcataaagt
     1981 gatggcgtac agcgacgcgg gtagcgcatt tatcttcggt tcgctggtcg ggccgaaaat
     2041 ggacacgctg tttgatggcg cagaatttat ctttggtttc agggtattac cggcaattat
     2101 cttcgtcact gcactggtga gtattctcta ctacatcggt gtgatgggga ttttaattcg
     2161 cattctcggc ggtatattcc agaaagcatt aaatatcagc aagattgagt cattcgtcgc
     2221 ggtcaccacc attttcctcg ggcaaaacga aattccggcg atcgtcaaac cctttatcga
     2281 tcgtctgaat cgcaatgaat tatttacagc gatttgtagt ggcatggcct cgattgctgg
     2341 ttcgacaatg attggttacg ccgccctggg cgtacctgtg gaatatttgc tggcggcatc
     2401 gttaatggcg atcccaggcg ggatcttgtt tgcccgcctg ttaagcccgg ctacggaatc
     2461 ttcgcaggtt tcctttaata acctctcttt caccgaaaca ccgccaaaaa gcattattga
     2521 agccgctgcg acaggggcaa tgaccgggct gaaaatcgct gcgggtgtgg caacagtggt
     2581 gatggcattt gttgccatca ttgcgttaat taacggtatt atcggcggcg ttggcggctg
     2641 gtttggtttt gaacatgcct cgctggagtc cattttaggt tacctgctgg ccccactggc
     2701 gtgggtaatg ggtgttgact ggactgatgc gaatcttgcc gggagtttga ttggacagaa
     2761 actggcaata aatgaatttg tcgcttatct caatttctca ccctatctgc aaacggctgg
     2821 cactctggat gctaaaaccg tggcgattat ttccttcgcg ttgtgcggtt tcgctaactt
     2881 tggttctatc ggggtggtgg tgggggcgtt ttctgcggtt gcgccacacc gtgcgccgga
     2941 aatcgcccag cttggtttac gcgcgctggc ggcggcgaca ctttctaacc tgatgagtgc
     3001 caccattgcc gggttcttta ttggtttagc gtagtgtgcg atttatgagc cggataagat
     3061 gcggtcaagc gtcgaatccg gcaacattgt tacaccattg gcactaatga aagcgcgtta
     3121 tcggcagaca gggtagggtt gtttaagctg ccgctggcgc gtgaaattgc cgcgcaggcc
     3181 atcgcaaaac gggcgctgtc acggaaattg tttccttcca gaaagctata taccaggccc
     3241 gccataaaac catcgtcagc accaaaactg tctactgtcg tatgcgctgg tgcggtcagc
     3301 aaaaattgtt ctccatcctt ttcgctgcaa taaactgact catcgggcaa ataaacaaac
     3361 agttgctgaa caccttgctg atgcaacgca ttcactgcag cattacggtc agcatcgctg
     3421 gtgatcgcct gtccccataa aatttccagc tccggtaaag tgggtttcag ggtgtgaata
     3481 tgcgccagcc agtgtttgat tttgcccgct ttgaattctg aaacggtatc gacaaacacc
     3541 gggatctcac cagcgagggt aaagacccat tccagcgcct cggctgtcag gttgcaatca
     3601 gccagtacca cgcccgcatg acgaagtaaa tcgcgcgacc cgttcaataa ttgcggcgat
     3661 aactgttcca gcagatgggt atcgttaatc gccagcacgg tttcatcgtc tcgattggca
     3721 attgccagat acgtcgatgt gctttgacca tgtaaacgaa cgcagccgga gacattcacg
     3781 ccggcgcggc gcgtttcttc caggagcatt tcgccataaa agtcatcgcc aatcactgaa
     3841 agcaaatgga cgtcgcggcc taacagcgcc agattgtggg cgatattgcg tccaacgccg
     3901 cctgccgagc aatgaattgt accgggatga gaagccgctt gcgggtaatg gatatccgcc
     3961 atcccgcgaa tatccatatt gattgtcccc accactacgc agtattcctg ctcggtgaga
     4021 atgtaacctt tgcctttaat ccgccctttg cgcattaaat ccataatatg cgccgcaacg
     4081 cgcgaacgac tgatttgcag catgtccgca atttcgttct gctgaatcag cgggttacgc
     4141 cgtaaaattg caaggatctc cttttcccgg ttattcatct tcaggctacc gctttttcag
     4201 tttgttgcgc tttcagccag gcgatctctt ctgcccagat atccgggttg atggtttcaa
     4261 ggatcagcgg aatgccgtcg aaacggtcgt cctgcatgat ccagcggaac gcatcatgac
     4321 cgatattgcc ttcaccgagg ctatgatggc ggtcaacgcg gctgccaaag gtgcttttcg
     4381 catcgttaag gtgcatcccg cgcagatact taaagccgac aatacgggca aaatcagcga
     4441 acgttttctc gcattcggct ggagtacgca aatcataccc ggcagcgaaa gcatggcagg
     4501 tatcaatgca gacgccgaca cgggatttat cttccacgcc gtcgataatc gccgcgagat
     4561 gttcgaattt aaaccctaag ttactgccct gaccggcggt gttttcaatc accgcggtca
     4621 caccttgagt tttatccagc gcaatgttga tggattcggc aatacgcgca aggcaatcct
     4681 cttctgaaat ctgcatcaga tggctgccag ggtggaagtt gagcaaagaa agccctagtt
     4741 gttcgcaacg ctgcatttca tctataaagg catcgcgcga tttttccaga gcttcagtga
     4801 ccggatgtcc gaggttaatc agataactgt cgtggggaag aatttgcgcc gatgtgtagt
     4861 gatatttttc acaggcggct ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac
     4921 gccactgacg ttggtttttg gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc
     4981 gaattgcggc atttgccaga ccgccagcag cactaacgtg cgctccaatg tatttcatgc
     5041 gaggactcct gttaaacccg ctggaggaaa acggtaatga tagcgggtta acaggagcaa
     5101 gatgtagtgg tttatgcgat gacgctttga atcacatagt taatcgcacc accaccaaca
     5161 atcagccagg caaacagtac cagtgccatc agcagaggtt tcgccccggc ttttttcagc
     5221 gcgctgacgt gagtggtcag acccagcgcc gccatcgcca ttgccagcag gaaggtatcc
     5281 agcgttacca gcatgttcac cacgctctgc ggtaacaggt ggaacgagtt aaagatggca
     5341 actacgatga acaagatggc aaaccacgga atagtgattt tgcttttctc gccgctgttc
     5401 gccccagaca gctgtttaac acgcgccgcc agcaggatga ggaacggagc cagcatcatc
     5461 acgcgcagca ttttggaaat aactgctgcg ttttccgcat ccgggctgat ggcatgacct
     5521 gccgccacca cctgcgccac ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc
     5581 ggactaaacc attgagacat cagcggatat atcgccgggt agaggaaaat cgcgacggtc
     5641 ccgaagataa caacggttgc aacagccacg gttactttac tggcttccgc tttcactacc
     5701 ggctcagtcg ccagtaccgc ggcagcacca cagatactgc taccggcacc gatcaaccag
     5761 ctggtgtgct tatccagacc aaacactttc tgccccagga agcaagccag caggaaggta
     5821 ctggacagcg tcagcacgtc aatgatgatc ccactgatac cgacatcggc aatttgcgag
     5881 aacgtcagac ggaagccata aagaatgata cccagacgta ataaatattg cttggcaaac
     5941 agcacaccac cgtcacagct tttccagatg tgcgggtaaa tggtgttgcc taaaaccatc
     6001 cccaacaaga ttgcgagggt gagggcacta aacccggcac ccgcaaccgc gggaatggaa
     6061 cccccccaca gggcgacccc ggtgataact gcactcaggg ctaaccccgg aataaaatgc
     6121 cacagtgtac gatgttgttt ctgtaaggtg atattcgtca taaccctctc ctttaaccgg
     6181 gcataaggtt acgactaact ggtttaaaaa taaaattgat tatatattta taattaatct
     6241 ttataagtgg taagcgacta tgcacatcac cctccggcag ttggaagttt ttgcagaagt
     6301 attgaaaagt ggatcaacca cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt
     6361 gagcgcagcc ttgaccgacc tggaagggca gcttggcgtt caactgtttg atcgcgtggg
     6421 gaaaagactg gttgttaatg aacacgggcg gctgctctat ccgcgagcgt tggcactgct
     6481 tgaacaggcg gttgaaatcg aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta
     6541 tgccagtagt accatcggta actacattct gcctgcagtt atcgcccgtt atcgccatga
     6601 ttatccgcag ttgccgattg aacttagcgt tgggaatagc caggatgtga tgcaagcggt
     6661 gctggatttc cgcgttgata ttggctttat tgaaggaccg tgccacagca ctgaaatcat
     6721 ttctgaaccg tggctggaag acgagctggt ggttttcgcc gcgccgactt cgccgttggc
     6781 ccgtggtccg gtcaccttag agcagctggc cgctgcgccg tggatcctgc gtgaacgcgg
     6841 ttccggcacg cgggagattg tcgattatct attgctgtca catttaccga agtttgagat
     6901 ggcgatggaa ttaggtaact ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat
     6961 tagttgcctg tcgcgacgtg tgattgaaga tcaattgcag gcaggcacat taagtgaagt
     7021 tgcggtccct ctcccgcgcc tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca
     7081 cctttccaac gcgctacggc gctttctgga ctattgcgat cccgcgaatg taccgcgtta
     7141 aggtgctgta caagaacatg ctggtgctgt gtcgatttcg tgacgcagcg ccctcagcat
     7201 gcattcgcca gaaaagagat tggctgcttt acttataatc cctgggcgat catgaaggtg
     7261 tcttataacc gtgtatttct gccggaagga ttgccaatcg tctgctacaa tcgcgcctca
     7321 tttttaagat ggatagcatt tttgtatggt ttccgaaact aaaaccacag aagcgccggg
     7381 cttacgccgt gaattaaagg cgcgtcacct gacgatgatt gccattggcg gttccatcgg
     7441 tacaggtctt tttgttgcct ctggcgcaac gatttctcag gcaggtccgg gcggggcatt
     7501 gctctcgtat atgctgattg gcctgatggt ttacttcctg atgaccagtc tcggtgaact
     7561 ggctgcatat atgccggttt ccggttcgtt tgccacttac ggtcagaact atgttgaaga
     7621 aggctttggc ttcgcgctgg gctggaacta ctggtacaac tgggcggtga ctatcgccgt
     7681 tgacctggtt gcagctcagc tggtcatgag ctggtggttc ccggatacac cgggctggat
     7741 ctggagtgcg ttgttcctcg gcgttatctt cctgctgaac tacatctcag ttcgtggctt
     7801 tggtgaagcg gaatactggt tctcactgat caaagtcacg acagttattg tctttatcat
     7861 cgttggcgtg ctgatgatta tcggtatctt caaaggcgcg cagcctgcgg gctggagcaa
     7921 ctggacaatc ggcgaagcgc cgtttgctgg tggttttgcg gcgatgatcg gcgtagctat
     7981 gattgtcggc ttctctttcc agggaaccga gctgatcggt attgctgcag gcgagtccga
     8041 agatccggcg aaaaacattc cacgcgcggt acgtcaggtg ttctggcgaa tcctgttgtt
     8101 ctatgtgttc gcgatcctga ttatcagcct gattattccg tacaccgatc cgagcctgct
     8161 gcgtaacgat gttaaagaca tcagcgttag tccgttcacc ctggtgttcc agcacgcggg
     8221 tctgctctct gcggcggcgg tgatgaacgc agttattctg acggcggtgc tgtcagcggg
     8281 taactccggt atgtatgcgt ctactcgtat gctgtacacc ctggcgtgtg acggtaaagc
     8341 gccgcgcatt ttcgctaaac tgtcgcgtgg tggcgtgccg cgtaatgcgc tgtatgcgac
     8401 gacggtgatt gccggtctgt gcttcctgac ctccatgttt ggcaaccaga cggtatacct
     8461 gtggctgctg aacacctccg ggatgacggg ttttatcgcc tggctgggga ttgccattag
     8521 ccactatcgc ttccgtcgcg gttacgtatt gcagggacac gacattaacg atctgccgta
     8581 ccgttcaggt ttcttcccac tggggccgat cttcgcattc attctgtgtc tgattatcac
     8641 tttgggccag aactacgaag cgttcctgaa agataccatt gactggggcg gcgtagcggc
     8701 aacgtatatt ggtatcccgc tgttcctgat tatttggttc ggctacaagc tgattaaagg
     8761 aactcacttc gtacgctaca gcgaaatgaa gttcccgcag aacgataaga aataagtttc
     8821 ctcccttcct tgctaagccc tctcaaccga gagggctttt tcaattccat ttccctgaca
     8881 aatcatgcgg atataaaatt taacatttgg attgataatt gttatcgttt gcattatcgt
     8941 tacgccacaa tcaaaaaagg ctgacaaatc agaggctgtt ccggctttct gggatgatca
     9001 cctgcataaa aaataagtcc accgcgatgc tgccgtacgc aaggggacgt gaagaagatg
     9061 tgagcgataa cccattttat tttcgtagtt acctcatgga gatatggaat gtttaggttg
     9121 aaccctttcg tacggggcgg gctgtgtttg tccgctattt cttgtgcatg gcctgtgtta
     9181 gcggtcgatg atgatggcga aacgatggtt gtcactgcat cttccgtgga acaaaatctt
     9241 aaagatgcac cagccagtat cagcgtcatt acccaggaag acctgcagcg aaaaccggta
     9301 cagaatctga aggatgtcct caaagaagtg cctggcgtac aactgacgaa cgaaggggat
     9361 aaccgtaagg gcgttagtat tcgtggtctg gacagcagct acaccctgat tcttgtcgac
     9421 ggtaaacgcg ttaactcccg caatgccgtc ttccgccaca atgatttcga tctgaactgg
     9481 atcccggtcg attccatcga acgtattgaa gtggttcgtg gcccgatgtc gtcgctgtac
     9541 ggctccgatg cgctcggcgg tgtagtgaat atcatcacca aaaaaatcgg tcagaaatgg
     9601 tcgggtaccg ttaccgtcga taccaccatt caggaacatc gcgatcgcgg tgatacctat
     9661 aacggtcagt tctttaccag cggaccatta attgacggcg tgctgggaat gaaagcttac
     9721 ggcagcctgg caaaacgtga aaaggatgac ccgcaaaact caacgaccac cgataccgga
     9781 gaaacgccgc gtattgaagg attctccagc cgcgacggca atgtcgaatt tgcctggaca
     9841 ccgaatcaaa atcacgattt tactgccgga tacggtttcg accgtcagga tcgtgattcc
     9901 gactcgctgg acaaaaaccg cctggaacgc cagaactact ccgtcagcca taatgggcgt
     9961 tgggattacg gcaccagcga actgaaatac tacggtgaga aagtcgagaa caaaaaccct
    10021 ggcaacagca gcccgataac ttccgaaagc aatacggtcg acggcaaata cacgttgccg
    10081 ctgacggcga ttaatcagtt tctcacggtt ggcggtgaat ggcgtcacga caaacttagc
    10141 gatgcggtga acctgaccgg gggaaccagc tccaaaacgt ctgccagcca gtacgcgctg
    10201 tttgtggaag atgaatggcg gatcttcgag ccgctggcgc tgacgaccgg cgtgcgtatg
    10261 gacgatcacg aaacctacgg tgaacactgg agtccgcgtg cctacctggt ttataacgcc
    10321 accgacaccg taacggtgaa agggggctgg gcgacggcat ttaaagcacc ttctctgttg
    10381 caacttagcc ctgactggac gagcaattcc tgccgtggcg catgtaagat tgtgggtagc
    10441 ccggatctga aaccagaaac cagcgaaagt tgggagctgg ggctttacta catgggtgaa
    10501 gaaggctggc tggaaggggt tgaatccagc gttaccgttt tccgtaacga tgtgaaagat
    10561 cgtatcagca tcagccgtac gtctgacgtc aacgctgcac cgggctacca aaactttgtc
    10621 ggttttgaga cgggcgctaa cggacggcgc ataccggtat ttagctacta caacgttaac
    10681 aaagctcgta ttcagggcgt ggaaaccgaa ctgaaaattc cgttcaacga tgaatggaaa
    10741 ctgtcattca actacaccta caacgatggt cgtgatgtca gcaacggcga aaacaaaccg
    10801 ctatccgatc tgccgttcca taccgctaac ggtacgctgg actggaaacc gctggcgctg
    10861 gaagactggt cattctatgt ttctggtcac tataccgggc agaaacgagc cgacagcgcg
    10921 acggctaaaa cacctggcgg ttataccatc tggaataccg gcgcggcctg gcaggtgact
    10981 aaagacgtca aactgcgcgc aggcgtgctg aaccttggcg acaaggatct cagccgtgat
    11041 gactacagct ataacgaaga cggacgtcgt tactttatgg cagtggatta tcgcttctga
    11101 tgagaagatg cccggcgaac cgggcggact ttcacttcag taaatactgc gcatggaagc
    11161 gcaggtgatc ctctataaaa gaggcgatga agtagtaact gtgatcatat cccggctgaa
    11221 tacgcagcgt catcggccac gctttctggc gcgcggcttc tgccagaact gcgggttgca
    11281 actgatcggc aagaaactga tcattatcac cttgatcgat aagcgtcggg atcgcatcct
    11341 gcgcgttact ggcatacatc agcgcgcaac tgtcccattc cagccatgca tttttgtcct
    11401 cacctaaata gcggctaaac gctttgatcc cccacgggac gctgcacgga ttcacaatgg
    11461 gcgcaaaggc ggaaacgctg gtgtatttgc ccgggttttt cagcgccata atcagcgcac
    11521 cgtgaccacc cattgaatga ccgctaatgg cgcagcggtc gctgacatta aattgcgact
    11581 gaaccagcgc cggtaattca tcgcgcagat aatcatacat ccggtaatgc gtcgcccacg
    11641 gcggttgcgt ggcattaaga taaaagcctg cgccctggcc taaatcgtag ccatcgtcgt
    11701 tggcaacctg ttcgccgcgc gggctggtgt ctggcatcac cagtacaatc cccagttccg
    11761 ccgctacccg ctgggcaccc gccttggtgg tgaagttctc gtcattgcag gttaatccgg
    11821 aaagccagta cagcactggt ggtggggtgt gatcacgagg tggtgggaga aagatactga
    11881 acgtcatcgg gcagtttaag gtactggagt cgtgtcgcca gcgttgctgc cagccttcaa
    11941 aacagcggtg ctcttcgagc atttccatgc gtggctcctt gttgtgttgt ttgcaatttc
    12001 ttcatcatac cgtttattca atgtgctgtg agtaactttc acttccgtat ttgcataacg
    12061 atgttttaac atctgctgat gaaaggcagc ggcaattaca ataattatcg ctgtgaatac
    12121 tggattatgt gcgccgcctc acgcacaata atcaggctgt aaatcagctt aataactttg
    12181 cccccacgca gggcggaggc gtcacacctg caggagaaat cataaatgcc atcactcagt
    12241 aaagaagcgg ccctggttca tgaagcgtta gttgcgcgag gactggaaac accgctgcgc
    12301 ccgcccgtgc atgaaatgga taacgaaacg cgcaaaagcc ttattgctgg tcatatgacc
    12361 gaaatcatgc agctgctgaa tctcgacctg gctgatgaca gtttgatgga aacgccgcat
    12421 cgcatcgcta aaatgtatgt cgatgaaatt ttctccggtc tggattacgc caacttcccg
    12481 aaaatcaccc tcattgaaaa caaaatgaag gtcgatgaaa tggtcaccgt gcgcgatatc
    12541 actctgacca gtacctgtga acaccatttt gttaccatcg atggcaaagc gacggtggct
    12601 tatatcccga aagattcggt gatcggtctg tcaaaaatta accgtatcgt gcagttcttt
    12661 gcccagcgtc cgcaggtgca ggaacgtctg acgcagcaaa ttcttattgc gctacaaacg
    12721 ctgctgggca ccaataacgt ggctgtctcg atcgacgcgg tgcattactg cgtgaaggcg
    12781 cgtggcatcc gcgatgcaac cagtgccacg acaacgacct ctcttggtgg attgttcaaa
    12841 tccagtcaga atacgcgcca cgagtttctg cgcgctgtgc gtcatcacaa ctgattaaaa
    12901 ggcaggaacc atggagcgca acgtcacgct cgattttgtt cgcggcgtcg ccattctggg
    12961 gatcctgcta ttaaacatca gcgcctttgg gctaccaaag gcggcttatc tcaatcccgc
    13021 ctggtacggc gctattacgc cgcaggatgc atggacctgg gcatttcttg atctcgtcgg
    13081 ccaggtgaaa ttcctcacgc tttttgcgct gctgtttggt gcgggcctgc aaatgttgct
    13141 gccccgtggc agacgctgga tccagtcgcg gttaacgctg ttagtcttgc tgggttttat
    13201 tcacggttta ttgttctggg acggcgatat tctgctggct tacgggctgg tgggcttaat
    13261 ctgctggcgg ctggtgcgcg atgcgccatc ggtaaaaagc ctgtttaata caggcgtcat
    13321 gctttatctg gtggggcttg gcgttttgct gttattgggg ctgatttccg acagccagac
    13381 cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact ggaagcttca
    13441 cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact
    13501 gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg cgctgatgcg
    13561 cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg gttttgtgct
    13621 ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc agctggactg
    13681 ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg cgccgtttca
    13741 ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc gctttaagct
    13801 ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat tgcaaacgct
    13861 gatttgtacc atgctttttt accacctcgg tttgtttatg cattttgatc gcctggagct
    13921 gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta tctggctgcg
    13981 ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc gtgctgccgg
    14041 aacggcaata tctaaaacat caagataacg atctggatca cattcgttaa caaaacggct
    14101 gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg ctgacaaaat
    14161 agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata tgatcaccat
    14221 tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa
    14281 cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga gtgagctgga
    14341 ttatcggcca aatgccaatg cccaggcgct ggcaactcag gttagcgata ccattggcgt
    14401 ggtggtgatg gacgtttctg atgctttttt cggcgcgctg gtaaaagcgg tggatctggt
    14461 cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag cggaaaaaga
    14521 gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg ttcactcaaa
    14581 agcattgagt gacgatgaac tggcgcaatt tatggataac attcccggta tggtgttaat
    14641 caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc
    14701 ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt atctttcttc
    14761 ctgccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg cgttgaaaga
    14821 gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca tgccgggcgg
    14881 tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg tatttgctta
    14941 taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca ttgcgattcc
    15001 gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca ccgacccgca
    15061 attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg aactggcctt
    15121 gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc cgacgttagt
    15181 gcgtcgtcat tctgtcgcaa cgcgccagaa tgcggcggcg atcactaact caacaaatca
    15241 ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcttaacaa tgtgatagct
    15301 atgattgcac cgttttaacg ttgtaacccg tatgtaacag tgaataatca ctttcaccga
    15361 ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg gcatggaaga
    15421 acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct actgccccgc
    15481 cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca tgaataagaa
    15541 ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg cacacgctgc
    15601 tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt ctgtagtgcg
    15661 caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga tgaatgattc
    15721 tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga aaggggtgaa
    15781 ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg agaaagcgcg
    15841 tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg cgctggatag
    15901 ctacgacaaa gcctactacg ttggcactga ttccaaagag tccggcatta ttcagggcga
    15961 tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag acggtcagat
    16021 tcaattcgta ttgctgaaag gtgaaccggg ccatccggat gcagaagcac gtaccactta
    16081 cgtgattaaa gagctgaacg acaaaggaat taaaactgaa cagttacagt tagataccgc
    16141 tatgtgggat accgctcagg cgaaagataa gatggacgcc tggctgtctg gcccgaacgc
    16201 caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc
    16261 actgaaagca cacaacaagt ccagcatttc ggtgtttggc gtcgatgcgc tgccagaagc
    16321 gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg ctaacaacca
    16381 ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg
    16441 caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg tagataaaga
    16501 caacctggct gaattcagca agaaataaga ctgattgggc gcagtcgttt actgcgccct
    16561 tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt cttccgggga
    16621 atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg cacttgataa
    16681 cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa acggtgcagg
    16741 aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg gcaccatttt
    16801 attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa atggtatttc
    16861 gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata acatgtggct
    16921 ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa
    16981 agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg gcacattatc
    17041 cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt
    17101 gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc tgttcactat
    17161 tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata aaatggaaga
    17221 aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga tcgccaccga
    17281 accgctggca ggactgacga tggacaagat catcgccatg atggttgggc gttctcttaa
    17341 tcagcgtttt cctgacaaag aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct
    17401 aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata aaggggagat
    17461 cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga cgttatttgg
    17521 tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca ataaccataa
    17581 tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc gctcaacggg
    17641 aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc gcaactacaa
    17701 aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt gggtgattga
    17761 ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa
    17821 tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat taatgctcga
    17881 tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt taattgccga
    17941 actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg agttgttagg
    18001 gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa
    18061 aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag attaggggct
    18121 tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg gcggtattta
    18181 cgtcgttctt ttagttttgc tggcgattat tattttccag gacccaacat ttttaagtct
    18241 gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg cgctcggtgt
    18301 ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg tagggctggc
    18361 ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag tgttcccgga
    18421 aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg gtgcggtgat
    18481 cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca ttaccacgct
    18541 cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg tcggggcgtc
    18601 gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg tcgcgctggg
    18661 gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt
    18721 gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta acccggaagc
    18781 ggcaaaagta tctggtgtca acgtcggcct gaacctgctg atgatctacg cgttgtctgg
    18841 cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg ccaccaacaa
    18901 cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt
    18961 cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt ttaccgtcat
    19021 caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca tcaaaggggc
    19081 gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga agtgatgtga
    19141 ttgtggatat tgccggatgc gctgcatccg gcaatcggtg ccaaatgccg gatgcgtctc
    19201 tggttttaca acgttaccgg gtgttctttc tcgcctttct taaacttcac ttccccgagc
    19261 tcaatacaac ccaccgggca gacgtgacca caaagcagac aacccacaca tttctcggta
    19321 ttacaatgcg gtgtgcgggt tttctcgctc cattccatcg cctggtgacc gccgtcgtaa
    19381 caggaaatat aacagcgtcc acagccaaca catttatcaa gattgatacg gggatagaca
    19441 atataactgc ggtctaaatc ttccgccggg acaatattgt tattcgccag acctaccatc
    19501 tcctgcagcg aatcaaatcc ctgatcggcg agataatgcg acaacccgct tgccatatct
    19561 tccactatcc gatacccgta ctgcatgatg ccggtggtca cctgtaacgt tgctgcgccg
    19621 agcaataaaa actcagccgc atcctcccag gtttcaatgc cgccgatacc gctgattggg
    19681 aaatcgcgca gttctggatg ggtgcgcatt tgctgaatga agcgcagggc gatcggtttt
    19741 accgctttac cggaatatcc ggaaatactc gattttccgt taacgatcgg cataccgatt
    19801 ttctgattaa gatcgatatt ggtgatggat ttaacggtgt taatcgccgc aatgccatct
    19861 gcgccgccgc gcttcgccgc cagcgccact tcgcacatat caccgatatt cggcgtcatc
    19921 ttcgccagca ttggcagcgt ggaaccccgt ttcactgccc gacaatattt ttctaccagc
    19981 tccgggcttt gcccgacatc gctacccatc gcatgagaag tcatttgcgg acaggagaag
    20041 ttacactcga tcatatccgc gccagcttct tgcaccaggc gcgccagctc ctcccattgc
    20101 tgctcatttt cccccatgat cgaagcgatc aatactttgt ccgggtaatc ttccttcagc
    20161 tgacgcaggg cggccagatt ctcttccaac ggatgttcag caatctgctc catatttttg
    20221 aagccgataa aaccggtatc ttctttcacc agatgatcaa aacgcggcga gacttcgttg
    20281 gcgataaaaa agccgatcgt tttaaacacc acgccgcccc aacctgtgtc gtaggctttg
    20341 gcacacatct catagcagtt gcctacaggc gaagaagaga ggcagaacgg gttgggaaac
    20401 ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca tgagcaagct ccctctaaat
    20461 agtgatgaat cgcctcggcg gcttctttcc cggttttcac ggcatagacc acggttttgt
    20521 caccctcaac aatatcgcca gcagcaaaga cttgcggatc gcgggtctga taattttgtg
    20581 ttttgatggt gttacgctgc ggctctaact ccgcaaaggc atccagtctg gcatgctgac
    20641 cgacggcgag aataatttta tctgccgcca tcgtcagttc gcccggtaac cgtacatgct
    20701 taaacgtgac tttattgcct tccacggcta ctggcgtgaa tccatcgatg atcgaaacgc
    20761 ccagctcccg ggcgctggca aactcttttt cgcttgccgg aaactcatct aactcttcac
    20821 gcgctacgca ggttaccgcc tgacagccga gaactttcag cgtgctggct acgtccatcg
    20881 caacatcacc gccgccgata attaatgcgc tttgtggaat gctgatatcg ccttgcgcct
    20941 gtcgtgcacg ttgcaggaag tccacggcaa tctcaacgtt gctatgctca aacagcgata
    21001 acccggaacc gctcgataac cccacggtga ccagtaccgc gcggttttct gctttcagct
    21061 gctcaagggt gagtgtgtta ccgacttcgt tgttgcactt aatggtcacg cccatttttt
    21121 caatacgggc gatctctgca tccagcactg attgcggtaa ccggaattgc ggaataccgt
    21181 tacgcaacca gccaccgggg tgcgcttctt tctcataaat cgtgacgtca taaccctggt
    21241 ttgtcagtgt tacactggcc tgcaatccgg caggacctgc gccaataatc gcgactttgc
    21301 cgagcgtttt agtaccgggc tgataaattt ccatcccagt ttgttgttca aaatcagtaa
    21361 caaaacgctg taagcgaccg atatcaatgg gagtatcaac accggcacgg gtacaaccgc
    21421 tttgacataa tttctccgtc gggcacactc tggcacaaac ggcaccgagg gcattatttt
    21481 cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta gattgagcga ataaatttcc
    21541 ccggatcggt ctgtgccggg caagcctgac tacagggagc gtcatgacat aataaacagc
    21601 gagaggcttc tttaatcgcc agtaaaggcg taaaagccgg agtgagttca tccagataat
    21661 tttgttgcgg catgtgatct tccttaatgc tatgagtaat taaggcgaac gctaaatata
    21721 ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccag tcacttactt
    21781 atgtaagctc ctggggactc actcacttgt cgccttcctg caactcgaat tatttagagt
    21841 ataaatattg cgtgctgata ttaaggctat gcgcgtgggg aaatttatac tgtgaaagga
    21901 ttcacaaata gctgcggttc agaatgttat atccgcagct tattgagtga agtaataatt
    21961 attctcaata attaacagaa aagagaaaat attaacgata gaaaataaga taaacaatca
    22021 tgccgataga taaaattccg aagccgatat atgccaccat ataagcgaaa aagagtccgg
    22081 gattttttgc tcgccagaca tacaccggtt ccggtgcatt tttaacccgt ttatcaatcg
    22141 cgcctgcgcg ccagcctttc catgcttcgc ggaaacaaaa tatcggaata aggaaaaaaa
    22201 cggcaacgac aaaaaactgc tgtacatcca taactacttt ccttgtttct tttgtaattc
    22261 aagtaactgt tcgggtgtga cggcgggata cccctgcgca tcttccggca cctggtgcat
    22321 agccggaata gggaccagcg gccctaaaaa ccgcggttca cgtttaaaaa tataaaggtc
    22381 agccagcgca ccgaaacggg cggcaaattc gcgaatacgt actgacagca tatctttcgg
    22441 tgacggtacg gcataacact gagcctgaat ccccatatgc agcgcaataa ataatgcccg
    22501 ctcacagtgg aaacgttggg tgataataat gaaatcatta gtatcaaaaa ctttgcgtgt
    22561 acgcacgatg gaatccagcg tacgaaagcc tgcgtaatcg agaacaatat ctgatgggtc
    22621 gacaccagca gcgattaaat ctttgcgcat ggtcatcggc tcattataac tttgcaatgc
    22681 gttatcgccg ctcagtaata gataatttac cttaccgctg ttataggcat taatcgctcc
    22741 ttgaatgcgg tagcgataat actgattaat tacgccagta cgataatatt ttgctgttcc
    22801 gagcaccaca ccgacctggc ggtaggggag atcctgcaat tcgtcgtaga tataaggcgc
    22861 ggttttccag ctcatccagc gatcgaggcc gagcacagtc aacagcagca agccgatcag
    22921 gactaacagg ctgaggaaca cgcgctttaa catgcaggag actcgatcta aggaaaattt
    22981 ccatcaggct actttacccg ctggttttgc gcaagaaacg gcaaactgtt tgccgggatt
    23041 ttcgacacca ggagagggct ggcaagccgg acaaggcgtt cacgccgcat ccggcaccag
    23101 gcttaagcga gaagtacgcg gtcgatactg tgacagccga gagctttcag tgttgcggag
    23161 gtggcatccc actgaatcaa cggcgcatcg gctttttctg ccagaaccgc gcgctggata
    23221 tccgggtaat cataaccctt gagattcaat agaattaacg ctccctgcaa cggtggcaga
    23281 gtcgggttga atgcggcgtt ttcagcgtag ctgccgctga aaatacgacc gtctttacat
    23341 tccagcgcga caccgcttgg cgacttactg taaggcatgt gcgaacggtt tgccgctgca
    23401 atcgccgcct gagaaagcgc atcaccagtc agcgcatagc cgtgatcctg ctcgtccatc
    23461 agcagcgttt taatctccag atctttcggc ccaaaggcat ccggcagata gtcacgcagc
    23521 gcgtgtgcct cgcggcccgg cagatgaata cgcagatcca gaccgctgtt cagttcattc
    23581 ataaactgac ggcagtgacc acaaggcgtg tagttaacgg tgatggctgc aagcgctttt
    23641 tcaccactca accaggcgtg gctgatcgcg ctttgttcgg catgaacggt ttgctgcatt
    23701 gtcgcaccaa taaattccat attggcaccg aaataccagg ttccgctcac accgcgcgca
    23761 attgcgccaa cattaaaatt cgacaatggc gtacgcgcac aggccgccgc cagcggaagt
    23821 agtgcgaatg ccagcgcgtc ttcgtccagc cccgttgcgc tcttcagcga tgagacttgc
    23881 tccccggtca acaaagcggg gaagtacttg tctgccagaa taggttccag tgcagattgc
    23941 aagttatccg caagttgggc aaaagcggtt tgaaaacgtg gatgcatggc gttgcctcat
    24001 aatcatgtaa tgggataaca gtttacgaac ccgttatggc tttatatgtg atctgaatct
    24061 cattaattat gcaacgcgtg aaacttacat caaaaatgcg cgacgcatcg caaattttag
    24121 cccattactg ccagaataat cgggaaaagg aacggtgcga tcagcgaagt aattatcccg
    24181 cataacacca gcgctagcga actaaatgca ccttcctgat aatccagctc ggcgcagcgc
    24241 gccgtaccga gggcgtgcga ggcagtcccc attgccagac cgcgcgcagc tttggtacga
    24301 atacgcatcg cattaagcaa tgtatggcca aataccgcgc cgaggatgcc gacgaaaatc
    24361 acgcaaactg cgctgattgc cggaataccg ccaatactgc cgccaaccgc cattgcaata
    24421 ggcgtggtga ctgatttcgg caggattgac gcggcgattt ccggtgaagc gcccatcaac
    24481 aatgccacgg aagtccccgt caccatcgca accacgctgc cgataaaaca gatggtgatg
    24541 atcgatttcc agcgcgcgcg gatctggtgt agctgctcat ataaaggata agccaacgcg
    24601 acaaccgccg gttgcagcaa atcgtttagc acttcgctac ctttaaagta gctgtcgtaa
    24661 gagatgccgg tgagcattaa aaaaggaatg atcaccacca ttgctaccag caacgggtta
    24721 agcaggggga acttataccg tgccgccagt ttgcgggcgg caaaaaagac aatcaacgtc
    24781 agcggtaatg accaccagat attcgccatc attcttctga tcctttctga cctacgactt
    24841 tacgttcacc gtgtaccagt tgtgaactcc agctcaccac cagaaaaacc accagcgtac
    24901 tgactgcaca ggaaaccact accgggccaa actgtgcgcg gagcaaatca aaatattgca
    24961 tgacgcctac gccaatcggc acaaacaata gcgccatata gcgaatcagt acgtagcacc
    25021 ccggattgac ccattttgcc ggaagaattt gcaaggccag caggacaaac aggatcagca
    25081 taccgatgat gctgcccgga atggttaccg gtagcaggga agcaatgaaa atgcctgcat
    25141 acaggcaggc ataaatcagg acgaaagcgc gtaaatattg ccagataatg ttcagtgtct
    25201 tactcataat caacacccca gatgagatgg attcatcata caattaattc gtaaaacgtg
    25261 ctaccgatca cattaagaaa tcagcgctta ttttttcgct ccccggcatt acgcaatgcg
    25321 caacgaactc aggcatacat caagcataga gagggattac agtttgcagc aatccctctt
    25381 tgcagacctt taatccgttg accataggcg ctaacatggc gcctcatttt tttgcgggtg
    25441 aagatatgcg tgtgttactg gctccgatgg agggtgtgct tgactctctg gtgcgtgaat
    25501 tgctgaccga agttaacgac tacgatctgt gcatcaccga gtttgtccgc gtggtggatc
    25561 aactgctgcc ggtaaaagtc tttcatcgca tttgccctga gctacaaaac gccagccgga
    25621 caccatctgg tacgctggtg cgcgtgcagt tgttaggtca gttcccacaa tggctggcag
    25681 agaacgccgc ccgtgcggtg gagttaggtt cctggggcgt ggatctcaat tgcggctgcc
    25741 cgtcgaaaac ggttaacggt agcggcggcg gcgcgacgtt actcaaagat cctgaactca
    25801 tctaccaggg tgcaaaagcg atgcgtgaag ctgtaccagc gcatttgccc gtcagcgtaa
    25861 aagtgcgtct gggctgggac agcggtgaga agaaatttga aatcgccgat gcggttcaac
    25921 aggctggcgc tacggagctg gtggtgcatg gacggacaaa agagcagggt taccgcgcgg
    25981 agcatattga ctggcaggcg attggcgaga ttcgccagcg gctgaatatt ccggtgattg
    26041 ccaacggcga aatctgggac tggcagagcg cgcaacaatg cattgcgatc agcggctgcg
    26101 acgcagtgat gattggtcgc ggggcgctca atattcccaa cctgagccgg gtagtaaaat
    26161 ataacgaacc gcgaatgccg tggccggagg tggttgcttt gctgcaaaaa tatacccgtc
    26221 tggaaaagca gggcgatacc gggttatatc acgttgcgcg gattaaacag tggttgagtt
    26281 atttgcgtaa aaaatacgat gaagcaacgg aattatttca gcatgttcgg gtgttgaata
    26341 attcccctga tattgcaagg gctattcagg caattgatat cgataagctg cgataatagt
    26401 ggaatcgcag tttatcagag acaatgaatc gcttattcag cttcatcatc ttgttgcgct
    26461 cctttcatga gctaagccac atattgccac tggcgcaagg agcgcgcagg gggaggacaa
    26521 tcgcctcctc cccctgcacc cccgggctct ggcgaacaaa atcgccgc
//
LOCUS       DAZGXZ010000065        26273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000065 DAZGXZ010000000
VERSION     DAZGXZ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.065"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHKJF_002806"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHKJF_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP0950139.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHKJF_002807"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHKJF_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP0950140.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHKJF_002808"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHKJF_002808"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP0950141.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHKJF_002809"
     CDS             complement(1154..1621)
                     /locus_tag="ACHKJF_002809"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP0950142.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKJF_002810"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHKJF_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP0950143.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKJF_002811"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHKJF_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP0950144.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKJF_002812"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHKJF_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP0950145.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKJF_002813"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHKJF_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP0950146.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKJF_002814"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHKJF_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP0950147.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKJF_002815"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHKJF_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP0950148.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKJF_002816"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHKJF_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP0950149.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKJF_002817"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHKJF_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP0950150.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKJF_002818"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHKJF_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP0950151.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKJF_002819"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHKJF_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP0950152.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKJF_002820"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHKJF_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP0950153.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKJF_002821"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHKJF_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP0950154.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKJF_002822"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHKJF_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP0950155.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKJF_002823"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHKJF_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP0950156.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKJF_002824"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHKJF_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP0950157.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKJF_002825"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHKJF_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP0950158.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKJF_002826"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHKJF_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP0950159.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKJF_002827"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHKJF_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP0950160.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKJF_002828"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHKJF_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP0950161.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKJF_002829"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHKJF_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP0950162.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKJF_002830"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHKJF_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP0950163.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHKJF_002831"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHKJF_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP0950164.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKJF_002832"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHKJF_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP0950165.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKJF_002833"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHKJF_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP0950166.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKJF_002834"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHKJF_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP0950167.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKJF_002835"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHKJF_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP0950168.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKJF_002836"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHKJF_002836"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP0950169.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKJF_002837"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHKJF_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP0950170.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHKJF_002838"
     CDS             15761..16129
                     /locus_tag="ACHKJF_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP0950171.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGGGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKJF_002839"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHKJF_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP0950172.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKJF_002840"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHKJF_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP0950173.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKJF_002841"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHKJF_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP0950174.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKJF_002842"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHKJF_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP0950175.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKJF_002843"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHKJF_002843"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP0950176.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKJF_002844"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHKJF_002844"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP0950177.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKJF_002845"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHKJF_002845"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP0950178.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKJF_002846"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHKJF_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP0950179.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23279
                     /gene="smg"
                     /locus_tag="ACHKJF_002847"
     CDS             22806..23279
                     /gene="smg"
                     /locus_tag="ACHKJF_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP0950180.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23308..23850
                     /locus_tag="ACHKJF_002848"
     CDS             23308..23850
                     /locus_tag="ACHKJF_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP0950181.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHKJF_002849"
     CDS             23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHKJF_002849"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP0950182.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24432..25250
                     /gene="aroE"
                     /locus_tag="ACHKJF_002850"
     CDS             24432..25250
                     /gene="aroE"
                     /locus_tag="ACHKJF_002850"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP0950183.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25247..25504
                     /locus_tag="ACHKJF_002851"
     CDS             25247..25504
                     /locus_tag="ACHKJF_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP0950184.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25480..26034)
                     /locus_tag="ACHKJF_002852"
     CDS             complement(25480..26034)
                     /locus_tag="ACHKJF_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP0950185.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6731 a         6247 c         6816 g         6479 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccacagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgccagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagcgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg gtagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcttccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgcttcgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaggt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggta atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaagtct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagc tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt ccgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtggttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgttacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccagg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagattactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaatat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacagc gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagcatcc ccacttatct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgc catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtgg
    15841 cggcgaacca ttgatactgg atgatgattc tcacgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcaacgga gagaagcaat
    15961 tcagcttctg gatattctca aaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acccgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggtgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gtatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ctcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactgaca ggattacact cgcgccgtta aataaccaac tggagttttt
    16621 atgagccgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgagtagag aaaaataaga aagggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggccttct gctttcaggc gtttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgt cagtaatact tcttctttag ttggtgctgg tgcggctgct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaagtc gatcccgcca
    17101 attaataagc ccagaggtgg catgatgata tcggcaacca gtgaagatac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacgtgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaatgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacgctc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttcg
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcacgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttccacaatg gtcgaacctt gtggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcgttac caatcagcgg gccgccataa taggctttaa ccacagcgag gctgacttta
    18301 ccctcagcga agttcaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagtg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgactcgcag gtcaaattta tcctgcaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtgatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggcag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcgcaa agttcggcat cagcggtacg ttgcaaaaag
    18901 gctttaatct gcaggctatt ctcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgctttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgcttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc cagaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagccgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccaa caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttggcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcattaaact cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgttt atcaaccctt taagttgcgg acgcttaatt
    19741 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca aaccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gcaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgccagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagataacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagaccat ctgcctccag ttcaaatggt ttaccgtcgc
    21241 ggtccagggc gcgaatttta actttctctg cgcgtggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc atacatcgtc tcgaacatat
    21481 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agaaatttgg ctgcgtttaa tgagtatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcatat tgatgcggtt gtattgcagc aaactgggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttcag gcgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccatagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacga ttattttgcg aaactctggc gacgcgtgga gtgacaatta caagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agcggcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtttgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct agtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg
    23041 aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcagatt caggtgctca
    23101 accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc gctggataac gcagagttcg
    23161 aactggatga tctgaaatgg gtaatcctga tggtgttgtt caatattccg ggctgcgaaa
    23221 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
    23281 catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat
    23341 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
    23401 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
    23461 gatggacata ttgtcaaagt tctggagggg caggtttgcc ctgcatgtgg cgcaaatctg
    23521 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
    23581 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgccggacg
    23641 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
    23701 ccggagtgtc aatttgccat taacttcaaa cccatagctg gagaatgccc tgagtgtcat
    23761 tatccactac tcatcgaaaa gaaaaccgcg cagggtgtga aacacttttg tgccagtaaa
    23821 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
    23881 tatcgcagct gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc
    23941 cgttttcggt gtcgggtgcg atcctgatag cgaaacagca gtgatgcgac tgttggagtt
    24001 aaaacagcgt ccggttgata aggggctgat tttaatcgcg gcaaattacg agcagcttaa
    24061 accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg
    24121 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
    24181 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
    24241 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
    24301 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
    24361 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
    24421 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
    24481 cattcattca tcagcaattt gctcagcaac tgaatattga acatccctat gggcgcgtgt
    24541 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttcgtgct ggtggtaaag
    24601 gtgcgaatgt gacggtgcct tttaaagaag aggcttttgc cagagcggat gagcttactg
    24661 aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcctgc
    24721 tgggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc
    24781 gtcctggttt acgtattctg ctcatcggcg ctggtggagc atctcgcggc gtactactgc
    24841 cacttctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
    24901 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
    24961 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
    25021 cggcgatccc gtcatcgctc attcatccag gcatttattg ctatgacatg ttctatcaga
    25081 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
    25141 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
    25201 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
    25261 ccagtttccg gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
    25321 cgtgaatggc atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac
    25381 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga
    25441 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
    25501 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
    25561 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
    25621 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
    25681 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
    25741 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
    25801 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
    25861 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
    25921 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
    25981 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
    26041 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
    26101 tagtatgcaa aaaagcaccg ttttgtgtgc gaatgcggca aaaagggtga aaaaacaaca
    26161 aacagaaaaa aagatcaaaa aagtacttgt gcaaaaaatt gggatcccta taatgcgcct
    26221 ccgttgagac gacaacgtga aacacttcac aagatggtcg taacaacgaa gag
//
LOCUS       DAZGXZ010000066        26224 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000066 DAZGXZ010000000
VERSION     DAZGXZ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.066"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..549
                     /locus_tag="ACHKJF_002853"
     CDS             <1..549
                     /locus_tag="ACHKJF_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HFP0950186.1"
                     /translation="IDHELAVAGQIIPATDEITMKYPISEAFFRDMFINHPFIPFGHE
                     EFFIKGMVCGFNGDFMTACHVLIPQLENSLRYVAKIKGEEPSQLHGDGSQERNGLKGL
                     LDNPLIIEAFGVDIIGNLQALLVDKIYGDLRNQLAHGYVPAGYYNQPPCIFAWWLVLH
                     ILMNPTARYWQATYGQESEAQT"
     gene            complement(768..1169)
                     /locus_tag="ACHKJF_002854"
     CDS             complement(768..1169)
                     /locus_tag="ACHKJF_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0950187.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKTAVVDPDVIWSLPDG
                     EIRRYDSRLNIICRECRMSEVMQRILAFYQG"
     gene            complement(1210..2175)
                     /locus_tag="ACHKJF_002855"
     CDS             complement(1210..2175)
                     /locus_tag="ACHKJF_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950188.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRPNVIGSKWPDDPTENTT
                     EITTENKNTFRPEASQPDPQTAEQDFLIRHPGAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTVWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            complement(2156..2677)
                     /locus_tag="ACHKJF_002856"
     CDS             complement(2156..2677)
                     /locus_tag="ACHKJF_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0950189.1"
                     /translation="MKITPEQAREALDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            complement(2661..2888)
                     /locus_tag="ACHKJF_002857"
     CDS             complement(2661..2888)
                     /locus_tag="ACHKJF_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0950190.1"
                     /translation="MLKVDAITFFGSKTKLANAAGVRLASVAAWGILVPEGRAMRLQE
                     ASGGELLYDPKVYDEYRKTKRAGRLNNENHS"
     gene            2969..3376
                     /locus_tag="ACHKJF_002858"
     CDS             2969..3376
                     /locus_tag="ACHKJF_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0950191.1"
                     /translation="MDTRTLGQRVLARRKELRLTQREAARLAGVAHVTISQWERDETQ
                     PVGKRLFALADALKCSPTWLMFGDEDKAPVPAQELHVETELTPNHKELIELFDALPSS
                     EQEALLSEMRARVENFNKLFEEMLKARKNKSIK"
     gene            3544..3699
                     /gene="ydfA"
                     /locus_tag="ACHKJF_002859"
     CDS             3544..3699
                     /gene="ydfA"
                     /locus_tag="ACHKJF_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0950192.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            3701..3802
                     /gene="ydfB"
                     /locus_tag="ACHKJF_002860"
                     /pseudo
     CDS             3701..3802
                     /gene="ydfB"
                     /locus_tag="ACHKJF_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            4763..4951
                     /gene="dicB"
                     /locus_tag="ACHKJF_002861"
                     /gene_synonym="ftsT"
     CDS             4763..4951
                     /gene="dicB"
                     /locus_tag="ACHKJF_002861"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0950193.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            4948..5139
                     /gene="ydfD"
                     /locus_tag="ACHKJF_002862"
     CDS             4948..5139
                     /gene="ydfD"
                     /locus_tag="ACHKJF_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0950194.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVINQDNIEISAGL"
     gene            5233..7704
                     /locus_tag="ACHKJF_002863"
     CDS             5233..7704
                     /locus_tag="ACHKJF_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0950195.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPNETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPVTKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMQGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRACDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            7777..8028
                     /locus_tag="ACHKJF_002864"
     CDS             7777..8028
                     /locus_tag="ACHKJF_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0950196.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            8063..9343
                     /locus_tag="ACHKJF_002865"
     CDS             8063..9343
                     /locus_tag="ACHKJF_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFP0950197.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(9531..10551)
                     /gene="rspB"
                     /locus_tag="ACHKJF_002866"
                     /pseudo
     CDS             complement(9531..10551)
                     /gene="rspB"
                     /locus_tag="ACHKJF_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
     gene            complement(10563..11777)
                     /gene="rspA"
                     /locus_tag="ACHKJF_002867"
     CDS             complement(10563..11777)
                     /gene="rspA"
                     /locus_tag="ACHKJF_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP0950198.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWTEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(11983..12309)
                     /gene="ynfA"
                     /locus_tag="ACHKJF_002868"
     CDS             complement(11983..12309)
                     /gene="ynfA"
                     /locus_tag="ACHKJF_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP0950199.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALYGMLIIV
                     AGWGRT"
     gene            12444..12785
                     /gene="ynfB"
                     /locus_tag="ACHKJF_002869"
     CDS             12444..12785
                     /gene="ynfB"
                     /locus_tag="ACHKJF_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP0950200.1"
                     /translation="MKITLSKRIGLLAILLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            12820..13380
                     /gene="speG"
                     /locus_tag="ACHKJF_002870"
     CDS             12820..13380
                     /gene="speG"
                     /locus_tag="ACHKJF_002870"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP0950201.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13383..14093)
                     /gene="ynfC"
                     /locus_tag="ACHKJF_002871"
     CDS             complement(13383..14093)
                     /gene="ynfC"
                     /locus_tag="ACHKJF_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP0950202.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            14201..14506
                     /gene="ynfD"
                     /locus_tag="ACHKJF_002872"
     CDS             14201..14506
                     /gene="ynfD"
                     /locus_tag="ACHKJF_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP0950203.1"
                     /translation="MKLSTCCAALLLALASPVVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            14705..17131
                     /gene="ynfE"
                     /locus_tag="ACHKJF_002873"
     CDS             14705..17131
                     /gene="ynfE"
                     /locus_tag="ACHKJF_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFP0950204.1"
                     /translation="MSKNDRMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAATV
                     QQASEKVIWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVIIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPER
                     RTFPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            17192..19621
                     /gene="ynfF"
                     /locus_tag="ACHKJF_002874"
     CDS             17192..19621
                     /gene="ynfF"
                     /locus_tag="ACHKJF_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP0950205.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKVKE"
     gene            19625..20242
                     /gene="ynfG"
                     /locus_tag="ACHKJF_002875"
     CDS             19625..20242
                     /gene="ynfG"
                     /locus_tag="ACHKJF_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP0950206.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            20244..21098
                     /gene="ynfH"
                     /locus_tag="ACHKJF_002876"
     CDS             20244..21098
                     /gene="ynfH"
                     /locus_tag="ACHKJF_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP0950207.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            21141..21755
                     /gene="dmsD"
                     /locus_tag="ACHKJF_002877"
     CDS             21141..21755
                     /gene="dmsD"
                     /locus_tag="ACHKJF_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP0950208.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            21914..23206
                     /gene="clcB"
                     /locus_tag="ACHKJF_002878"
     CDS             21914..23206
                     /gene="clcB"
                     /locus_tag="ACHKJF_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP0950209.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(23159..23854)
                     /gene="bioD"
                     /locus_tag="ACHKJF_002879"
     CDS             complement(23159..23854)
                     /gene="bioD"
                     /locus_tag="ACHKJF_002879"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0950210.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(23979..25199)
                     /gene="mlc"
                     /locus_tag="ACHKJF_002880"
                     /gene_synonym="dgsA"
     CDS             complement(23979..25199)
                     /gene="mlc"
                     /locus_tag="ACHKJF_002880"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP0950211.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(25334..>26224)
                     /gene="ynfL"
                     /locus_tag="ACHKJF_002881"
     CDS             complement(25334..>26224)
                     /gene="ynfL"
                     /locus_tag="ACHKJF_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0950212.1"
                     /translation="NIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQI
                     GARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSSA
                     PFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEHA
                     VIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHLT
                     PVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWPK
                     HHEQSPAARNFRIHLLNALR"
BASE COUNT         6987 a         6515 c         6231 g         6491 t
ORIGIN      
        1 atcgaccatg agttggcagt agctgggcaa attattcctg ctacagatga gatcacaatg
       61 aaatatccaa tctcggaggc gttttttcgc gatatgttca tcaatcatcc cttcatcccg
      121 tttgggcatg aagagttttt catcaagggt atggtttgcg gtttcaatgg agattttatg
      181 acggcatgcc acgtgttgat tccccagctc gaaaatagtc tgagatatgt ggctaaaatt
      241 aaaggtgagg aaccatcgca gcttcacggg gacggttcgc aagagcgaaa tggactcaag
      301 ggattactag ataatccact gattattgaa gctttcggcg tagacataat tggcaatctt
      361 caggccttac tggtagataa aatctatggt gatttacgaa atcaactagc tcacggatat
      421 gtgcctgccg gttactacaa tcaacctccc tgcatttttg catggtggct tgtattgcac
      481 attctaatga accctacggc gagatactgg caagccactt atggccaaga gagtgaggct
      541 cagacttaaa gtgaataagt ttacgaagtt ggcacttccg gatgcatgtc catatgagtt
      601 gaggcgattt ggttaataag gttgagtgct gctgaaatta ttcgatgtcc gcttttggca
      661 caaagcggac aaccacgcca gatctaccct gtgccatgaa aaatgagaat gtcaattcac
      721 tcctgaacta atgcttttta atctagtaac gtctaaaata cctaacatta tccctgataa
      781 aatgccagta tgcgctgcat aacttcactc atccggcact cgcgacagat tatgtttagg
      841 cgactgtcgt agcgacgtat ttctccatca ggtaacgacc agataacgtc cggatcaacc
      901 acagctgttt tcttcacctt tgccctcgag agttttttgc gggcgttttg ccagtcctta
      961 cgcgcctgtt cagacgggaa taacccgtag ccggagttgt atacatcgcc actggctacc
     1021 agctctctgg caagaacgct catcagatat ctagtcgcac ctgttttagc ttccagttgc
     1081 cgtaacgtct cacgaccgct ctggcgcacg agttccacca cctgcccttt aattttttcc
     1141 cgctcttctt gtgtaaaaac ttttgccaca agtccccctt aaaattacct catgacctga
     1201 aatcaaaact tatcctctga aaccaggcgg aatttctgta tccggttcag aaatatgatt
     1261 aacacaacgc tgtacaggtg aacgccccag gcggatgacc agttcgtccc atttttcgcg
     1321 gagctttgac gggctcatga tgttttttac ccagaatgga tcccgctgta cccgaccaaa
     1381 catttcacaa atttgtctgt ggcttctgcc atccagcatc cgcattgtgc gcacatcatt
     1441 ggcccagaca gtccagttag gctcttttgg tcgcatgatc tcgccatcat cactggcggc
     1501 ctgttcgtag agacccacga tccgccccca tatccactgc gcacacgcca gatcctcctg
     1561 gctaccccac tggcgttttt tcgcactaaa cacaactgcg ccagggtgcc ggattaaaaa
     1621 atcctgttca gccgtctgcg ggtccggttg cgaagcttcc ggacgaaaag tgtttttatt
     1681 ctctgtagta atctctgttg tattctctgt aggatcatcg ggccattttg acccgatgac
     1741 attgggtcgt tttgaaccaa tggagcgttt cattttgacc tcttccatcg tgtcattttg
     1801 acctgatgga gcggcgcatt ttgaaccgat ggattcgctc aatttgccat catctaaaag
     1861 ctcgctccca tagttaatcg tgtagaaatt ggtcatatcg cgctttgatt tattgagctt
     1921 ttcacaacgc aaaagcccca gcgttttcag acttgcaaac gcgcgcttta acgttgactc
     1981 tgaccagaat gggaactgtt ccaaccattg ttccgttgtg ttgtaaatcc agcgaacacc
     2041 atcacattcc atgccggaat tggtatctct caaccaataa tgcaactgct gcaacacaat
     2101 ggcttcattt aagccaatct tcatcgcaag ctgtgtgttt ataaccagtg ggcgttcagc
     2161 aaaaagaagg ctcataattc catccagctt tttgttggta ttgctgtcga tacgcaagtt
     2221 tgaaagcaat tgctttttct ataagttcgt cagtttcacg atccactacg gcaggatcag
     2281 caaaaagcag tccggactcc accacatcgc catattcttt gtttaacccg gcgatcatgt
     2341 acgtgatgct ttttccgtca ctaatttcac gatacaacct gaaatcatta atccggatag
     2401 cctccataat tgcaggcact agcgccgtga acttttcacg cttatccctg gtgtcgatag
     2461 ccttccagcg ttcgaatatc ttcactcgat taacgccaag cgctcgctga tcaaccgcgc
     2521 caccttcatc tgtgacacgc tgaacatcga tgttcgggcg ctctttcaaa gcccagaatg
     2581 cttcagtgat taatatcgtc gcctgctcct gtgtcattcc tggtcgacat atccaggcat
     2641 ccagagcctc acgagcctgt tcaggagtga ttttcattgt tcaaccgccc cgcccgcttc
     2701 gtcttacgat attcgtcata aaccttggga tcatacagaa gctccccgcc agatgcctcc
     2761 tgtagacgca tcgcgcgacc ttcaggaacc agtatccccc aagcagcaac acttgccagt
     2821 ctcactcctg cggcattggc aagctttgtt ttgctgccaa aaaaagtaat tgcgtcaact
     2881 ttaagcatca aagccccctc ttgttagact tttctaacat tattgtgcgc gggatatcta
     2941 agtcaagaaa aattagaatt acctaactat ggatacaaga accctaggcc agcgagttct
     3001 ggcgcgacga aaagaattac gcttaacaca acgagaagct gcgcgcctcg ctggagttgc
     3061 tcacgtcaca atttcacaat gggaaagaga cgaaacccag ccagtcggaa aacgattgtt
     3121 tgctttagcg gatgctctga agtgctcacc tacatggcta atgtttggtg acgaagacaa
     3181 ggcaccagtg cctgcacaag aacttcatgt ggaaacagag ctaactccca accacaaaga
     3241 attgatcgaa ttattcgatg ctcttccatc ttccgagcag gaagccttgc tgtctgaaat
     3301 gcgcgcaaga gtagaaaact tcaacaaact cttcgaagaa atgcttaaag cgcgtaaaaa
     3361 taaatcaata aaataacatt cttttcaagt gattagttgc gcccactctt tttgttaggc
     3421 taatctaaca aaaacacctg cctctcatgt taggttattc taaattactt tccatcaaga
     3481 caccgcacgg tgttctcagc aaacagttcc gctaccccgg cgttaagggg aaatgaggtc
     3541 agcatggata ctatcgatct tggcaacagc gaatctctgg tatgtggcgt gttccccaac
     3601 caggacggca cgttcaccgc gatgacgtat accaaaagca aaacgtttaa aaccgaaaat
     3661 ggtgcccgtc gctggctgga aagaaactca ggtgagtgat atggatttcg acacaatcat
     3721 ggaaaaggct tacgaagaat acttcgaagg ccttgccgaa ggcgaagaag ctctcagctt
     3781 cagtgagttt aaacaggcac tcagaataag aatgtgctct cacaatgacg cggagcacaa
     3841 atatgagaag caaaatcaga ccgcagaaaa ttttgttctg gaacccggag aaacgctttt
     3901 caaaattccc gttacgtgcc ccatttgcgg ttttacatca gaagaacttg acgactcctg
     3961 taacaatcag gaaacaacca agtatgtcga agatgatacc gagtgcgcac gaagaacgat
     4021 tatatccacg agtccaaact ccagggccaa taaatctcac tttgagaggg tgattaatcc
     4081 actcccccaa accaataaaa aagatgccgg aggacaaaaa acccaatgga acaacgggat
     4141 atctgtcaaa aaaagacgtt ccattaaaga caaacaacgc agcgccaaca actgtaagcg
     4201 ctttatacca gtaatcaatt ttcatgttct taagcggatt tattggtggt tgcgacattg
     4261 cttaatgaat ccttaaaact gtggtgattt taaggatacc acctcgcctg acgtggttaa
     4321 aagcaggcac acaacacgaa agcgcacggc gaagttcgtc tcactgtacg gtgtcgttaa
     4381 atttaattcg accgtgcgct tccggttgtg gcaacccgca aaatggcgcg gcggtaagta
     4441 tggcggggtt attccttccc cgttgaggac accgggttgt caggttgacc atacgcttaa
     4501 gtgacaaccc cgctgcaacg ccctctgtta tcaattttct ggtgacgttt ggcggtatca
     4561 gttttactcc gtgactgctc tgccgccgtt tttaaagtga attttgttat gcggtgaatg
     4621 cggctaagcg cacgcggaac agttaaaacc aaaaacagtg ttatgggtgg attctctgta
     4681 tccgacgtta attgttaact ggttaacgtc acctggaggc accaggctct gcatcacaaa
     4741 atttattgtt gaggacgcga taatgaaaac gttattacca aacgttaata cgtctgaagg
     4801 ttgttttgaa attggtgtca ctatcagtaa ccctgtattt actgaagatg ccattaacaa
     4861 gagaaaacaa gaacgggagc tattaaataa aatatgcatt gtttcaatgc tggctcgttt
     4921 acgtctgatg ccaaaaggat gtgcacaatg aattcagcat ttgcgcttgt tctgacagtt
     4981 tttcttgttt ccggagtgcc agttgatatt gcagtcagtg ttcacaggac aatgcaggag
     5041 tgtatgactg cagcaaccga acagaaaatt cccggtaact gttacccggt cgataaagtt
     5101 attaaccagg ataatatcga aatctcggca ggtctttaaa acagttccgt aataaatatc
     5161 cggtttcatt cttatatgcc agcaatggca gggatttgtt catccttaaa tctgtcatga
     5221 ggttaaaaca aaatgagtaa agtctttatt tgcgccgcta ttcctgacga actggcaaca
     5281 agggaagaag gcgctgtggc tgtagccaca gccattgaag ctggcgacga acgccgtgct
     5341 cgagcaaaat ttcactggca attcctggaa cattatccgg ctgctcagga ctgcgcttat
     5401 aaatttattg tctgcgagga taaacctggc ataccccgcc ctgccctcga ttcatgggat
     5461 gctgaatata tgcaggaaaa ccgctgggat gaggagtctg cttcttttgt cccgaatgag
     5521 actgaatccg atccgatgaa cgtcactttt gacaagctgg cccctgaagt acagaacgct
     5581 gtcatggtta agttcgacac atgtgaaaac atcaccgttg atatggttat tagcgcacag
     5641 gaattgttgc aggaagacat ggcaacattc gacggacata tcgttgaagc gttgatgaaa
     5701 atgccagaag ttaacgccat gtatccggag cttaagttgc acgccattgg gtgggttaag
     5761 cataaatgta ttcctggtgc taaatggccc gaaattcagg cagagatgcg catctggaaa
     5821 aaacgtcgcg aaggtgaacg caaggaaacc ggaaaataca cgtctgttgt tgatctcgca
     5881 cgcgccagag ccaatcaaca gtacactgaa aattcaacag gaaaaatcag cccggtcatt
     5941 gctgccattc atcgcgaata caagcagaca tggaaaacac tggatgacga actggcctac
     6001 gctctctggc ctggtgatgt ggatgccgga aacattgacg gcagcatcca tcgctgggca
     6061 aaaaaagaag ttatcgacaa cgaccgcgaa gactggaagc gtatctcggc atcaatgcgc
     6121 aaacagcctg atgcccttcg ctacgaccgc cagactattt ttggccttgt ccgtgagcgt
     6181 ccgatcgaca ttcacaaaga tcccgtagca ctgaacaaat atatctgcga atacctgacg
     6241 acaaagggcg tgtttgagaa tgaagaaaca gacctgggca ctgttgatgt tctccagtca
     6301 tcagaaacac aaactgatgc agtggaaact gaggtatctg atatcccaaa aaatgaaacc
     6361 gcgccggaag ctgaaccatc tgtagagcgt gaggggccgt tctatttcct cttcgcagat
     6421 aaggacggag aaaaatacgg tcgcgcaaac aaactttctg gtctggataa ggcactggct
     6481 gctggcgcca ctgaaatcac aaaagaagaa tattttgccc gaaaaaatgg cacatacacg
     6541 ggcttaccgc aaaatgtaga taccgctgaa gattcagaac aaccagagcc gataaaagtt
     6601 accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgctgat
     6661 aagttgcttg ctgcatcacg tggtgaattt gttgaaggga ttagtgaccc gaatgatccg
     6721 aaatgggtta aggggatcca gacccgcgat tctgtgaacc agaaccagca tgaatcggaa
     6781 cggaactacc aaaaagcgga acaaaacagt ccaaatgcgt tacaaaacga gccagtaacg
     6841 aaacagcctg aaccagtagc gcaacaggaa gtggaaaaag tctgcaccgc ctgcggtcag
     6901 accggcggcg gcaactgccc tgattgtggc gcggtgatgg gcgacgcaac ataccaggaa
     6961 acattcgatg aagagtatca ggttgaagtt caggaagatg atccggagga aatgcaaggc
     7021 gctgaacatc cacacaagga gaacactggc ggcaatcagc atcacaatag cgataatgaa
     7081 actggcgaga cggcagatca cccaattaag gtgaacggtc atcacgaaat cacatccacc
     7141 agcagggcgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa tcctgatgcc
     7201 ccgatcatct caataggtgc aatatttttc gatccgcaaa ccggagatat gggaccggaa
     7261 tttagtaaga ctatcgatct ggaaactgct ggcggagtca ttgatcggga caccattaaa
     7321 tggtggctta agcaatcacg cgaagcgcaa tctgccatta tgaccgatga aatcccgtta
     7381 gatgatgcac tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt
     7441 caggtttggg gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg
     7501 caggggatcc cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag
     7561 ctggggaaag ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat
     7621 aatgcacttg atgacgcccg ttaccaggca aaatacgttt cagttatctg gcaaaaactg
     7681 atcccgagtc aggctgattc ttaatgttca actgtcgccg gttgtgactg gtattctgca
     7741 accggcgctc gtctgatgta agagataaag aaatcgatga gcgaagtaat catgattgtc
     7801 tctcccggca aatgggtatc cgaagagcag ttaattgcgc tgaaaggaat aaaaaaaggt
     7861 acgttaaaaa aggcccggga aaaatcgttt atggaaggaa gggaatataa gcatgtcgct
     7921 catgacggta tgccatggga taacagtcca tgcttttaca acctggaaga aattgatcgc
     7981 tggattgagc gccaggcatc agcgagacca agacgtcatc ttacttgact aaaagccaca
     8041 ctaactaatg agagaagttg aaatgaaata tccgacaggc gtggaaaacc atggagggaa
     8101 attacgtatc tggtttgttt ataaaggcgt aagagtcagg gaaaatctgg gggttcctga
     8161 cacagcaaaa aacaggcgcg ttgcaggtga actacgctcc tctgtttgtt acgcaataaa
     8221 aactggtgtt ttcgactatg caaaacagtt tccctcctca cgcaatctgg aaaaatttgg
     8281 tgaggcccga caagatttaa ccataaaaga actggctgaa aaatttctgg cactgaaaga
     8341 aactgaagtc gccaaaacat cactcaacac ataccgtgcc gtcatcaaaa atatcctgag
     8401 cataatcggt gaaaaaaatc ttgcctcatc gattaataaa gaaaaattac tggaggttcg
     8461 taaagagtta ctgactggat accagatccc caaaagtaac tatattgtta cacaaccagg
     8521 gagatcggct gtaactgtaa ataattacat gacaaatctt aacgccgtgt tccagtttgg
     8581 tgttgataac ggttacctgg cagataatcc gtttaagggg atctcgccat taaaggaatc
     8641 aagaaccatt ccggatcctc tttcgcggga agaatttatc cgtcttatcg atgcgtgcag
     8701 aaatcagcaa gcaaaaaatt tatggtgtgt ttctgtttat actggcgttc gccctggtga
     8761 gctgtgtgca cttggatggg aggacataga tctgaaaaat ggaacaatga tgatcaggag
     8821 aaatttagca aaagaccgtt tcacggtacc aaaaacacag gcgggaacca atcgggtcat
     8881 tcatcttatt aagccagcaa tcgacgctct ccggagtcag atgacattaa cgagactgag
     8941 caaagagcat atcattgatg ttcacctcag agagtatggc agaacagaaa aacaaaaatg
     9001 cacctttgtt tttcaacctg aagtgtcagc gagagtaaaa aattatggtg accattttac
     9061 cgttgactca ataaggcaga tgtgggacgc agcgataaaa cgtgccggac tccgccatcg
     9121 aaaatcatat cagtcgagac atacttatgc ctgctggtcg ctgacagctg gtgctaaccc
     9181 ggcatttata gcaaaccaga tgggccatgc agatgcgcaa atggtatttc aggtatacgg
     9241 aaaatggatg tctgaaaaca ataatgcaca ggtagctttg ttaaatacac agttaagcga
     9301 gtttgcccca accatgcccc ataacgaagc aatgaaaaat taatttaata tttatcaaat
     9361 agttaacacg catgactctt gaaatccata aattcaagcg cagtgcccag ccatcccgat
     9421 actgctgctt tcaccaaatc cttagtgctt ctttcatgtt tttctattgt cataatggtt
     9481 atctctaaaa aagaggtaag atgcgtacta cttactcgcc gttattggta ttattcagaa
     9541 aaagtgagta agactttgca gcaatgtttt tgatcctgtt caaataaact aatggcatca
     9601 gcaacatgct ggaaatcaaa cgtatgggta attaattttt ctggtttaat taacccttta
     9661 cttaaccagt cgataacaac cggaaattta tttgcattta agcgtgaaga gaaaatagag
     9721 agttcttttc cggtaattcc ttgctgaatc acttcagacg gttcactgga gaagcccatc
     9781 aatacaatac gtgccgcagg agaagccagc gttacggcct ctttcaggat agaaggatga
     9841 caagccgcat cgataattaa tgtcggcttg atgccttttt cagcgaaaat ctcgccaagc
     9901 ggtgtctggc tgttattaat cgcccagtct gcccccgctc tctttcgctt tttccagtcg
     9961 ttcatcaatg cgatcggcaa caatcacatt tttaacgtta tagacgcctt ttaatacctg
    10021 aacgatcgtc aggccgattg gaccggcacc ataaaccaga acggtatcat tttcagtcgg
    10081 ttgaccatga ccggtaacgt tagccgcaat ggtaaaaggt tcgatcatca ccgcatattg
    10141 atcggccact gcttcaggaa ttttccacgc attttttgcc ggaaccacgg catattcact
    10201 gaaaccaccg tcagcgtgca cacctaatac agcaagtgtc gtacaaacgt tcggcttacc
    10261 tatagagcac ggatagcaat gcccacagct gaccaccgga tcgacagcaa cgcgttcacc
    10321 gactctggcg ctttccacac cttcacccac cgcatcaatg acgccaaaga attcatgacc
    10381 aatgacgcgc ggatatttcg caaaaggatt atgcccacgg taaatatggc tatctgaacc
    10441 acaaattccg gcaagtttca cttttactcg tacttcaccc gctgacgggg tgggtatttc
    10501 acgttcgata atcgacagtt gattcggttt ttcaattaaa atgcttttca ttaccttact
    10561 ccttaccagt tccacagcgt gccatcttcc agacgtgcga ctggcagata agcaggttca
    10621 tagggatatt tcgccgccag cttttcatcg aattcgatgc caagacccgg tttgtctccc
    10681 ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag catttgttcg
    10741 gaataaccca tgtactcctg gacaccgaaa ttggggaccc acagatcaaa gtgcagcgcc
    10801 gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg tacctgatac
    10861 agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg ggtcagcgtg
    10921 gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca gatgctgttg
    10981 aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg gaagcattcc
    11041 tggttttccg caggcgtcgg gtcttccatc cagaacatgc gataatcttc aatgctttta
    11101 ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag caaatgttca
    11161 ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc gaggtatttc
    11221 tccgtcgacc acagctgctc ttccgtccac tgtcctttgg ttgcgggttc ataagccaga
    11281 cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca ctgcacgcgg
    11341 atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc atcaatactg
    11401 tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc gagtaactgg
    11461 taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc aaccgctgaa
    11521 atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa aaactgccag
    11581 atatcttcga tacggtgcgc atcgcgacca ataagctgcg gacaaaggtg atcctgcaaa
    11641 taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc cgtaataccg
    11701 tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca
    11761 gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat gaaatctacg
    11821 caactgagcc actaccatac aagtataaag atcgaaaaaa gccggagtga tcacaaaaaa
    11881 aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc ttttgtcagc
    11941 tgacacttcc tcttatctta ttgataaaat ggatttatgt tcctacgtgc gcccccagcc
    12001 cgcaacaatg atcaacatgc cgtaaagcgc aatcaacgca cccgtccagt cataaagagt
    12061 cagtttcacg ccatccacaa cgcgcagcca catcaacgcc gtgcagacat aaacgccacc
    12121 ataagccgcg taaacacgcc cactcgccgc tggatgcaac gttaacaacc agacaaacag
    12181 cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa
    12241 ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata gtaacgttgt
    12301 tttaatcatc tttgtctctt attgacatca tgtatagtta tagggcgaca taatatcatc
    12361 aatataaaca ccctcctggt acgttttgcg tccgcagtgg atggtgtaga atcaccttta
    12421 atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc ggcctgctcg
    12481 ctattctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa actaacaaac
    12541 tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg gaaaaagagc
    12601 aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa aaagagtggg
    12661 ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg
    12721 cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc gttattaccc
    12781 cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc ccacagtgtt
    12841 aagctacgcc cgctggagcg tgaagattta cgctatgtac atcaactcga caacaacgcc
    12901 agtgtgatgc gttactggtt tgaggaaccc tacgaagcct ttgttgaact ctctgatctg
    12961 tatgataagc atattcacga tcagagcgaa cggcgctttg tggtggaatg tgacggcgaa
    13021 aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgcgc agaatttcag
    13081 ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc caaattagca
    13141 atggactatg gctttaccgt tctcaatctc tataagctgt atctgatcgt tgataaagag
    13201 aatgaaaaag cgattcacat ttaccgcaag cttggctttt cggttgaagg tgaattgatg
    13261 cacgagttct ttattaatgg tcaatatcgt aatgccattc gcatgtgtat attccagcat
    13321 cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac cgcacaatag
    13381 cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc gggttttact
    13441 ccttcatcaa caatgattag ctgacagtcc accggattag cgtgactgtc atattcacag
    13501 ctctgtttta cattaccaac ccgttgatta ttcagtaaag taaccgctgt gtaatctaat
    13561 tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc gttacttttc
    13621 gtggttttac ccagcggata accctgatca tcatagcgat attccatctg catttgtttg
    13681 ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc ggcagaaggt
    13741 aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt ctcggcatca
    13801 cggtaataat tggcgtccag taccagagcg accacggtat tattttccag atccagtaat
    13861 tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt cgtcacttca
    13921 ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa
    13981 tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac ctctgtgcgg
    14041 tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt cacaacagtc
    14101 accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta cacgttaaat
    14161 gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct caacctgctg
    14221 tgccgcgctt ctgctcgccc tcgcctcacc cgtggtactc gccgcgccgg gatcctgtga
    14281 gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg aaagcagctt
    14341 cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg tcgtcggcca
    14401 ttgcgctaat gatacgcata aaattctcta tacccgcacc accagcggta acgtctctgc
    14461 tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaataca ttatcgactg
    14521 aacgccggat acgacacatc cggcgatttg aacatacaac ataatcccac cttattactc
    14581 atacccttct attgatatgg attaataatt cttaacccaa aatgggtaga ctccctctat
    14641 tgttagcgcg ctaaatattc aatatataaa catttatata acgataaaga acagggagtg
    14701 agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg ttaaatcaac
    14761 cgcgataggt tctctggcgc tggctgcagg cggtttttct ttaccgttca ccctgcgcag
    14821 tgcagcggca acggtacaac aggccagcga aaaagtgatc tggggtgcct gttccgtcaa
    14881 ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga cctgggtgga
    14941 aactgacaat accggcagcg atgagtacgg caaccatcag gtacgcgcct gtttgcgcgg
    15001 tcgctccatc cgccggcgta ttaatcatcc cgatcgcttg aattacccaa tgaaacgcgt
    15061 gggtacacgc ggtgaaggca aattcgaacg gattagctgg gatgaagccc tggatactat
    15121 cgccagtagc ctgaaaaaaa ccgtcgaaca atatggcaat gaggctgtat atattcagta
    15181 ctcttcgggg attgttggcg gcaatatgac ccgctcttcg ccatcagcct cggcggtcaa
    15241 acgcctgatg aactgctacg gcggttcact caatcagtat ggctcctaca gcaccgcgca
    15301 aatttcctgt gccatgccct acacctacgg cagtaatgat ggcaacagca ccacggatat
    15361 tgaaaacagc aagctggtcg tgatgtttgg taacaacccg gcagaaaccc gcatgagcgg
    15421 cggtggcatc acttatcttc ttgaaaaagc gcgcgagaaa tcgaacgcca aaatgattgt
    15481 tatcgatccg cgatataccg atacggctgc tggtcgtgaa gatgaatggc tccctattcg
    15541 cccgggcacc gatgccgcgc tggttgcggg tattgcctgg gtattgatta acgaaaatct
    15601 cgttgatcaa ccttttctcg ataaatactg cgtcggctat gacgaaaaaa ccttacccgc
    15661 agatgcaccc aaaaatggtc actataaagc ctatattctt ggtgaaggtg atgataacac
    15721 agcgaaaacg ccgcagtggg cttcgcaaat taccggtatc ccggtagacc gtatcatcaa
    15781 actggcgcgt gaaattggca cagcaaaacc cgcttatatc tgccagggct gggggccgca
    15841 acgccaggca aacggcgaac tgactgcacg cgctattgct atgttaccta ttttgacggg
    15901 caatgtcggc atcagcggcg gaaatagtgg cgcgcgtgaa tcgacctata ccattaccat
    15961 agaacgcctg ccggtgctgg ataatccggt caaaacgtca atctcctgct tcagctggac
    16021 ggatgctatc gatcatggtc cgcaaatgac ggcaatccgc gacggcgtcc gcggcaaaga
    16081 taaactggat gtgcccatta agttcatctg gaactacgcg ggaaataccc tcgttaatca
    16141 gcattctgac atcaacaaaa cgcatgaaat tctgcaggac gaatcgaaat gcgaaatgat
    16201 tgtgatcatc gaaaacttta tgacctcatc ggcaaaatat gccgacattc tgctgccaga
    16261 cctgatgacc gttgagcagg aagatattat tcctaacgac tacgccggta acatgggata
    16321 tctcattttc ctccagcctg tcaccagcga aaaattcgaa cgcaaaccga tttactggat
    16381 cctgagtgaa gtcgcgaaac gtcttggacc tgacgtctat caaaagttca cagaaggtcg
    16441 cacgcaggaa caatggttac aacatctgta cgccaaaatg cttgccaaag atccggcgtt
    16501 accgtcttac gacgaactga aaaaaatggg tatctataag cgtaaagatc ccaatggcca
    16561 ttttgtcgcc tacaaagcat ttcgtgacga ccccgaggca aatccactta aaacgccttc
    16621 cggtaagatt gaaatttatt ccagcaggct ggcggaaatt gcccgtacct gggaactgga
    16681 aaaagatgaa gtgataagcc cattgccagt ttatgcctca acctttgaag gctggaactc
    16741 ccctgaacgt agaaccttcc cactgcaact gtttggtttc cattacaaat cccgtactca
    16801 ctcgacctac ggcaatattg atctcctgaa ggctgcctgc cgtcaggagg tgtggatcaa
    16861 ccctatagat gcgcagaaac gtgggattgc caacggcgat atggtgcggg tgtttaacca
    16921 tcgtggcgaa gttcggctac cagccaaagt aacaccacgt attctccctg gagttagcgc
    16981 tatgggccag ggagcctggc acgaggccaa tatgtctggc gataaaatcg accatggcgg
    17041 ctgtgtgaat acgctaacca ctctgcgccc ttcaccactg gcgaagggaa acccgcagca
    17101 cactaatctg gtcgagatcg aaaaaatata acccacgaca accataaatt ctggcatgac
    17161 attttgtttg aaaagcaata agtgagtaat gatgaaaatc cataccacag aggcgctcat
    17221 gaaggctgaa atcagccgta gaagtctgat gaaaacctcc gcacttggca gtcttgcgct
    17281 ggcaagcagt gctttcactc tgccattttc ccaaatggtc cgggcggcag aggctccggt
    17341 agaagagaaa gcggtctgga gttcctgcac cgttaactgc gggagccgct gtctgttacg
    17401 tttgcatgtg aaagatgaca ccgtgtactg ggtggagtct gatacgacag gtgacgacgt
    17461 ctacggtaac catcaggttc gagcgtgttt acgcgggcgc tctatccgcc gacggatgaa
    17521 tcatcctgat aggttgaaat atcccatgaa gcgcgtcggc aagcgcggtg aaggtaaatt
    17581 tgaacggata agttgggacg aagccctgga taccatcagt gataatcttc ggcggatcct
    17641 gaaagattac ggcaatgagg ctgtacatgt cctgtacgga acaggcgtag atggcggaaa
    17701 catcaccaac tcaaacgtcc cgtaccgtct gatgaactct tgcggtggtt ttctcagtcg
    17761 ctatggcagc tacagtaccg cacagatcag tgccgcaatg agttatatgt tcggtgccaa
    17821 tgatggcaac agcccggatg atatcgccaa tacgaaactg gtcgttatgt tcggaaataa
    17881 cccggcagaa acgcggatga gcggcggtgg tgtcacttac tacgtcgagc aagcccgcga
    17941 acgttcaaac gcacgcatga tcgtcatcga tccacgttat aacgacactg ctgccgggcg
    18001 tgaagatgaa tggctgccca ttcgccctgg caccgatggc gcactggcct gtgcgattgc
    18061 ctgggtactg attactgaaa acatggtcga tcagccattt ctcgacaaat attgtgttgg
    18121 ttacgatgaa aaaacgctgc ccgccaacgc accacgtaac gcgcattata aagcctatat
    18181 tctgggcgaa gggcctgacg gcatagctaa aacgccggaa tgggcagcaa aaatcaccag
    18241 catcccggca gaaaaaatta tccagttggc acgagagatc ggttcagcaa aacctgctta
    18301 tatttgtcag ggttgggggc cacaacgaca ttccaacggc gagcaaacgt cccgcgctat
    18361 tgccatgctt tccgttctca ccggtaacgt cggcataaac ggcggcaact caggtgtacg
    18421 cgaaggtagc tgggatctgg gggtagaatg gttcccgatg cttgagaatc ctgttaaaac
    18481 gcagatttcc gtctttacat ggacagatgc catcgaccat ggtacggaaa tgaccgcgac
    18541 ccgtgatggt gttcgtggaa aagaaaaact ggatgtcccc atcaagtttt tatggtgcta
    18601 cgccagtaac acattgatca atcaacatgg cgacatcaat cacacccatg aggtgcttca
    18661 ggatgacagc aagtgcgaga tgattgttgg cattgaccac ttcatgacgg cctcggctaa
    18721 gtattgcgat atcctgttgc ccgacctgat gccaacagag caagaagacc ttatctctca
    18781 tgaatctgca gggaatatgg gctatgtgat cctcgcccaa cccgcaacct cagcaaaatt
    18841 tgaacgcaaa cccatctact ggatgctgag tgaagtcgcc aaacgcttag gaccagacgt
    18901 ttatcaaacc tttactgaag gtcgcagtca gcatgaatgg atcaaatatc tccatgcgaa
    18961 aacgaaggaa cgtaaccctg agatgcccga ctacgaggag atgaaaacga ccgggatctt
    19021 taagaaaaaa tgcccggaag aacactacgt cgctttccgc gcattccgtg aagatccaca
    19081 ggcaaacccg ttgaaaacac cttcggggaa aatcgaaatt tattctgaac gactggcgaa
    19141 gattgcagat acctgggaat tgaaaaaaga tgaaattatt catccccttc ctgcgtatac
    19201 cccaggtttt gatggctggg acgatcccct gcggaaaacc tatccactgc agttaacggg
    19261 cttccactac aaagcgcgta cccactccag ctacggcaat attgatgtgt tacagcaggc
    19321 ctgcccacaa gaggtgtgga tcaaccccat tgatgctcag gcacgcggta tccgtcatgg
    19381 cgataccgtg cgggtattta acaataatgg agaaatgctg attgccgcaa aagtgactcc
    19441 gcgtattctg cctggcgtta ccgccatcgg gcaaggtgcg tggcttaaag cggatatgtt
    19501 tggtgaccgg gtcgatcacg gcggcagtat caatattctg acctctcacc gcccttcacc
    19561 gctggcaaag ggaaacccgt cgcacagcaa tcttgtccag atcgaaaagg ttaaggagta
    19621 gcccatgacc acacaatatg gattttttat cgattccagc cgctgtactg gctgtaaaac
    19681 ctgcgaactg gcgtgcaaag attttaaaga tcttggcccg gaagtcagtt tccgccgtat
    19741 ttatgaatac gctggcggcg actggcagga agataatggc gtctggcacc agaacgtgtt
    19801 cgcttattat ctctccatct cctgtaacca ttgcgatgac cccgcatgta caaaagtctg
    19861 ccccagcggc gcaatgcata agcgtgaaga tggctttgtg gtggttgatg aagatgtttg
    19921 tatcggctgc cgctactgcc acatggcttg tccgtacggc gcaccacagt acaatgctga
    19981 aaaagggcac atgacgaagt gcgatggttg ttattcgcgc gtcgccgagg ggaaacaacc
    20041 catatgtgtc gaatcctgcc cgctgcgtgc acttgagttc ggtccaattg aagagctgcg
    20101 ccagaagcac ggcactctgg ctgccgtcgc gccactgccg cgcgcgcatt tcacaaaacc
    20161 caatatcgtt atcaaaccta acgccaacag ccgcccgacc ggcgatacca ctggttatct
    20221 ggctaatccg gaggaagtgt aaaatgggaa atggatggca tgagtggcca ctggttatct
    20281 ttaccgtatt gggtcaatgc gtagtaggcg cgctgattgt tagcggaatt ggctggttcg
    20341 ccgccaaaaa tgacgcagac cgtcagcgta ttgtccgtgg catgtttttc ctctggctgt
    20401 tgatgggcat cgggtttatt gcctctgtca tgcacctggg atcgccactg cgagcgttta
    20461 actcgctgaa tcgaatcgga gcttctggtc tgagtaacga gattgctgcc ggttccatat
    20521 tttttgccgt tggcggacta tggtggctgg tggcggtcat cggtaaaatg ccgcaagcgt
    20581 tggggaaact ctggttactt gtcagcatgg cgctcggcgt cattttcgtc tggatgatga
    20641 cctgcgtgta tcaaatcgac accgtgccaa cctggcataa cggttacact acgctggctt
    20701 tcttcctgac ggtattgttg agcggtccaa tacttgctgc cgcaattctg cgggcagctc
    20761 gcgttacatt taataccaca ccatttgcca tcattagcgt tttggcgctg atcgcttgtg
    20821 ccggggtgat tgtgctgcaa ggtttgtctc tggcgtctat tcactcatcc gtgcaacaag
    20881 ccagcgcact ggtaccagat tacgcctcct tacaggtctg gcgtgtggta ttgttatgcg
    20941 caggtcttgg atgctggctt tgcccgctca ttcgtcgccg tgaacctcac gttgccggac
    21001 ttattctggg gctaatcctc atacttggcg gtgagatgat cgggcgtgtg ctcttttatg
    21061 gcctgcacat gacagtagga atggctattg caggttaaca aattggtgcg gggcaacccg
    21121 cacatttcag gatgttagga atgacccatt tttcacagca agataatttt tctgtcgcgg
    21181 cgcgcgtgtt gggcgcgctg ttttattacg ctccagagag tgcggaagcc gcacctctgg
    21241 ttgcggtact caccagtgac ggctgggaaa ctcagtggcc tctaccagaa gcgtcattag
    21301 cgccgctggt gacggctttt cagacacagt gtgaagagac tcacgcccag gcctggcagc
    21361 gtttgtttgt cggcccgtgg gcactgccgt ctccgccgtg ggggtcggtc tggctggatc
    21421 gcgaatctgt gctgtttggc gattcaacat tggcacttcg tcagtggatg cgcgagaaag
    21481 gcattcagtt tgaaatgaag caaaacgaac cggaagatca ttttggttca ctgctgttga
    21541 tggctgcgtg gttggcggag aatggtcgcc agacagaatg cgaagaattg ctggcatggc
    21601 acctttttcc gtggtcaaca cgttttcttg atgtttttat cgaaaaagca gaacaccctt
    21661 tctaccgtgc actgggtgaa ctggctcgct taacgctggc gcaatggcaa tcacaactgt
    21721 taattcctgt cgcggttaaa ccgctgtttc gataggatca ccgtaatatt gccggatggt
    21781 gacgattacg cgcaatccgg caataatagg ttacagtgtc acgttttttt atctcttaaa
    21841 gcacgcactg cttttgcggc tggcctcttt tgccgcaaaa tagtcgcccg tgtttcattg
    21901 cccatttctg ctcatgcatc atctacacat ctatccggat ctgcgcacta tgttccgccg
    21961 tctgcttatc gcaacagtcg tcggtattct cgcggccttt gccgttgccg ggtttcgtca
    22021 tgcgatgctg ctactggagt ggttgttcct caataatgac tccggcagtc tggtcaatgc
    22081 agcgacaaac ctttccccct ggcgacggtt gctaactccg gcgctcggcg gactggcggc
    22141 gggtttgttg ctgatgggct ggcagaaatt tacccaacaa cgccctcatg cgccgaccga
    22201 ttacatggaa gcgttgcaaa ccgatggaca gttcgattac gcagcaagcc tggttaaatc
    22261 gcttgcctcg ctgctggtag taaccagcgg cagtgcaatt ggtcgcgaag gtgcgatgat
    22321 tcttttagct gcccttgccg cctcctgttt tgcccaacgt tttacgccac gccaggagtg
    22381 gaaattatgg atcgcctgtg gggccgcggc gggaatggct gcggcctatc gtgccccgct
    22441 tgctggcagt ttatttatcg ccgaagtgct gtttggcact atgatgttgg cctctctcgg
    22501 cccggtgatt atttccgccg tcgtggcatt gctggttagc aatctgatta atcatagcga
    22561 cgcgttactc tacaacgtac aactctcagt gacggttcag gctcgtgact atgcgctgat
    22621 tatcagtaca ggtgtgctgg caggtctgtg cggaccactg ttgttaacgt taatgaacgc
    22681 ctgccatcgt ggatttgtga gtctcaaact tgcgccgccc tggcaactgg cactaggcgg
    22741 gttgatcgtg ggtctgcttt ccctgttcac acctgcagtg tggggcaacg gctatagcac
    22801 cgtacaatcc tttttaaccg ccccaccact gttaatgatc attgccggga tcttcctttg
    22861 taaactgtgt gccgtgctgg cgagtagcgg ttccggcgca cccggtgggg tctttacacc
    22921 gacgctattt atcggtcttg ccattggcat gttgtatggt cgtagcctgg gattatggtt
    22981 ccctgatggc gaagaaatta cacttttact cggattgacc gggatggcga cactgttggc
    23041 ggcgaccacg cacgcgccga ttatgtcgac gttgatgata tgtgaaatga ccggggagta
    23101 tcagctactc cccggtttat tgattgcctg cgtaattgcg tcggtaattt cgcggacgtt
    23161 acaccgtgac tctatctacc gccagcacac tgcgcagcat agctaagcgg atgtattgcc
    23221 ccagttcacg ctgttcggcg cgcggcagat aaggcagttc accaatgagc ggtgccggaa
    23281 gttttttacc cagcacatca atgatttccg cataatgcgc cagtcctggg ttgattcggt
    23341 tagccaccca gccaatgagc ggcagcccgt cgttggcgat cgcctgagct gttagcagtg
    23401 catggttaat gcaaccttcc tgaataccga caaccatcaa caccggcagt tgttcctgca
    23461 ctacccattc agagagtgga cgcaaatcat tcatcagact gcgccagccg ccagtccctt
    23521 ctaccacgac atgatcgact ttttcggtca ggtttgccag gccgtttgaa atgagggtgt
    23581 aattgattgg gcaactgtgc gccacgctac tttcttcttc gcttaacgcg ataggattaa
    23641 ctgcttcata aggcagttcg atggttgaaa cactctgcaa caccagggca tctttattac
    23701 gcagcccttc gggtgtctct ttgctcccct tcgctacggg tttatatccc gcaaccgttt
    23761 ttccctggga ggctaacgct tgtagcaatg cgcgggaaac caccgttttc cctacagaag
    23821 tgtctgtacc ggtaataaag aaacgcttca gcatcactaa ctccaccgtt atgcttcaca
    23881 aatataaacc aggaaaataa ttaaccttga aagtctaagt tatgctttcc tggcccaaat
    23941 tgagatagcg caaattttgg tagaagagtt aaaaaatgtt aaccctgcaa cagacgaatc
    24001 aacaaagaac cgttatacat cgcgtctttt accagtgcag cccctgccat cgtgccctgg
    24061 ttagaaaact gagtactctc aacgctgatg tgctgactat acgcaggaag ggcctgctga
    24121 cggatgctgt ctgagatgac cgggaagagg atatctgccg ctttacttaa cggtgagcca
    24181 atcagtattt tttgtgggtt aaataaattc accatgatgg caagaatgcg ccctacatgc
    24241 gcgcccaccc cggtaatgat gtcttttgcc agcagatcgc cgcgcaatgc cgcctgacac
    24301 aatgagtcca cggttaacgg ttgtccatgt aacatcgagc tcatggattg attaagacgc
    24361 agctgtgcca gctcaagaat actgtccaca ctggcgatgg tttcgaggca gccgtgattc
    24421 ccgcaataac agcgtttccc atacgggtcg acctgtgtgt ggcctatttc cacgagacta
    24481 ctgctgcctg cgtgtagcag atgaccatcg gtaatgacgc ccgcccccac gttgtgatcg
    24541 ataaccacct gaatcacatc gcgcgccccg cgtgaggcac caaacaaggc ctctgccatc
    24601 gtccatgcgc tgatatcatg ctgaatataa accggtacgc cggtatgctg ctccagcgcc
    24661 tcgccgagcg gcatctcttt tacatcctcg tagaacggca tgcgatgtac aataccattt
    24721 tccgtatcaa taattcccgg caaggttatg gcaatcgaag ttagacgctc aagttttttc
    24781 tggtggcgaa taaaaaactg atcgatatgg gaaatgatac gatccagcaa tggcgagtca
    24841 tcttttaacg ccagttcctg cgactcttcc accaccagtt tgctgctcag atcgcgcaga
    24901 gcaaggaaaa tctccccgcg actaatgcgc agagaaagat agtgccaggc ttcagtttca
    24961 accaccagcc ccaccgccgg acggccacgg ttccccgctt ctttgatttc cagctcttgc
    25021 accaggtgtg cttcgagcat ctcacggaca attttagtga tactggcagg agccagttgc
    25081 gccagacggg aaagatcgat acgcgagact ggaccaagct gatcaatcag gcgataaacc
    25141 gcgcccgcgt tggtctgctt tatttgatca atgtgcccag gctggttttc agcaaccacc
    25201 gcatactccc tatattttcg cgctccgaaa taatctgtag gctatggtga agcacttcaa
    25261 tacgtgtcgt caaattttta cttaggcatg tgattaacag cacatttttc gggctttttc
    25321 gctgaaattt ccctcacctg agagcattca gcagatgaat acgaaagtta cgcgcagctg
    25381 gactttgttc atgatgtttc ggccagacca accacatttc agaaaccgca tcctcttctg
    25441 caatcggcac ccagcgcatt tcgttgagct gaacacgttt aaatgacgca ggcaaaattg
    25501 aaacacccag accggcggaa accagaccga tgatggtcat tgcctcgccc acctcctgag
    25561 tgatgacggg cgtcaaatgg taacgtcgca tcagcccgag aatatcgtca tacagccctg
    25621 tcccgacgtg cggatcaaaa aagacgaagg gttctttcgc cagttcagcc agcgttacat
    25681 tcgggttatt tgccagggga tgatcgtgcg ggatcatcgc cataagcggt tcatggacga
    25741 tgactgcgtg ttcaagcgtc tccggtaacg ctgtgttacg cagcaatccc atatccagcg
    25801 ttccttcaat gagcggagcg atttgctcac gagtgttcat ttcgcgggtt tgtaaatgga
    25861 catcgggata atcacggcga aacagcgata acgtatcgga caccgcccga ataaaaggag
    25921 cggacgaagt aaaaccaata cgcaactccc ccgcttcacc ctgatgcagt ctttcagcgc
    25981 gagcggcagc gtcatccacc atagacagga tttgccgact atctgcaaga aactgttttc
    26041 ctgctgccgt cagcaataca ctgcgattgg ttcgtgccag cagtcgggca ccaatttgtt
    26101 gctccagcgc ctgaatctgc tgacttagag gtggttgcga aatattcagg cgggcagcgg
    26161 cgcgcccgaa atgcagctct tccgcaacag caacaaagta acgcagatga cgaagttcaa
    26221 tatt
//
LOCUS       DAZGXZ010000067        25627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000067 DAZGXZ010000000
VERSION     DAZGXZ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.067"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..1022
                     /gene="ybhD"
                     /locus_tag="ACHKJF_002882"
     CDS             69..1022
                     /gene="ybhD"
                     /locus_tag="ACHKJF_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0950213.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            complement(1063..2058)
                     /gene="pgl"
                     /locus_tag="ACHKJF_002883"
     CDS             complement(1063..2058)
                     /gene="pgl"
                     /locus_tag="ACHKJF_002883"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP0950214.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDDRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2213..3031
                     /gene="ybhA"
                     /locus_tag="ACHKJF_002884"
     CDS             2213..3031
                     /gene="ybhA"
                     /locus_tag="ACHKJF_002884"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP0950215.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3032..4090)
                     /gene="modC"
                     /locus_tag="ACHKJF_002885"
     CDS             complement(3032..4090)
                     /gene="modC"
                     /locus_tag="ACHKJF_002885"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP0950216.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4093..4782)
                     /gene="modB"
                     /locus_tag="ACHKJF_002886"
     CDS             complement(4093..4782)
                     /gene="modB"
                     /locus_tag="ACHKJF_002886"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP0950217.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4782..5555)
                     /gene="modA"
                     /locus_tag="ACHKJF_002887"
     CDS             complement(4782..5555)
                     /gene="modA"
                     /locus_tag="ACHKJF_002887"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0950218.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5722..5871)
                     /gene="acrZ"
                     /locus_tag="ACHKJF_002888"
     CDS             complement(5722..5871)
                     /gene="acrZ"
                     /locus_tag="ACHKJF_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP0950219.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6000..6788
                     /gene="modE"
                     /locus_tag="ACHKJF_002889"
     CDS             6000..6788
                     /gene="modE"
                     /locus_tag="ACHKJF_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP0950220.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6856..8328
                     /gene="modF"
                     /locus_tag="ACHKJF_002890"
     CDS             6856..8328
                     /gene="modF"
                     /locus_tag="ACHKJF_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP0950221.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRLFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDLYRYALTKIN"
     gene            8589..9605
                     /gene="galE"
                     /locus_tag="ACHKJF_002891"
     CDS             8589..9605
                     /gene="galE"
                     /locus_tag="ACHKJF_002891"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0950222.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9615..10661
                     /gene="galT"
                     /locus_tag="ACHKJF_002892"
     CDS             9615..10661
                     /gene="galT"
                     /locus_tag="ACHKJF_002892"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP0950223.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10665..11813
                     /gene="galK"
                     /locus_tag="ACHKJF_002893"
     CDS             10665..11813
                     /gene="galK"
                     /locus_tag="ACHKJF_002893"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP0950224.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVVVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11807..12847
                     /gene="galM"
                     /locus_tag="ACHKJF_002894"
     CDS             11807..12847
                     /gene="galM"
                     /locus_tag="ACHKJF_002894"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP0950225.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13052..13804
                     /gene="gpmA"
                     /locus_tag="ACHKJF_002895"
     CDS             13052..13804
                     /gene="gpmA"
                     /locus_tag="ACHKJF_002895"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP0950226.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13962..15014)
                     /gene="aroG"
                     /locus_tag="ACHKJF_002896"
     CDS             complement(13962..15014)
                     /gene="aroG"
                     /locus_tag="ACHKJF_002896"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP0950227.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15330..15710
                     /gene="ybgS"
                     /locus_tag="ACHKJF_002897"
     CDS             15330..15710
                     /gene="ybgS"
                     /locus_tag="ACHKJF_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP0950228.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGAMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15824..16765
                     /gene="zitB"
                     /locus_tag="ACHKJF_002898"
     CDS             15824..16765
                     /gene="zitB"
                     /locus_tag="ACHKJF_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP0950229.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16762..17481)
                     /gene="pnuC"
                     /locus_tag="ACHKJF_002899"
     CDS             complement(16762..17481)
                     /gene="pnuC"
                     /locus_tag="ACHKJF_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP0950230.1"
                     /translation="MDFFSVQSILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17519..18562)
                     /gene="nadA"
                     /locus_tag="ACHKJF_002900"
     CDS             complement(17519..18562)
                     /gene="nadA"
                     /locus_tag="ACHKJF_002900"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP0950231.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18811..18886)
                     /locus_tag="ACHKJF_002901"
     tRNA            complement(18811..18886)
                     /locus_tag="ACHKJF_002901"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18851..18853),aa:Lys,seq:ttt)
     gene            complement(18920..18995)
                     /locus_tag="ACHKJF_002902"
     tRNA            complement(18920..18995)
                     /locus_tag="ACHKJF_002902"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18960..18962),aa:Lys,seq:ttt)
     gene            complement(19142..19217)
                     /locus_tag="ACHKJF_002903"
     tRNA            complement(19142..19217)
                     /locus_tag="ACHKJF_002903"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19182..19184),aa:Lys,seq:ttt)
     gene            complement(19221..19296)
                     /locus_tag="ACHKJF_002904"
     tRNA            complement(19221..19296)
                     /locus_tag="ACHKJF_002904"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19261..19263),aa:Val,seq:tac)
     gene            complement(19446..19521)
                     /locus_tag="ACHKJF_002905"
     tRNA            complement(19446..19521)
                     /locus_tag="ACHKJF_002905"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19486..19488),aa:Lys,seq:ttt)
     gene            complement(19524..19599)
                     /locus_tag="ACHKJF_002906"
     tRNA            complement(19524..19599)
                     /locus_tag="ACHKJF_002906"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19564..19566),aa:Val,seq:tac)
     gene            complement(19735..19810)
                     /locus_tag="ACHKJF_002907"
     tRNA            complement(19735..19810)
                     /locus_tag="ACHKJF_002907"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19775..19777),aa:Lys,seq:ttt)
     gene            complement(19975..20766)
                     /gene="cpoB"
                     /locus_tag="ACHKJF_002908"
     CDS             complement(19975..20766)
                     /gene="cpoB"
                     /locus_tag="ACHKJF_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP0950232.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20776..21297)
                     /gene="pal"
                     /locus_tag="ACHKJF_002909"
     CDS             complement(20776..21297)
                     /gene="pal"
                     /locus_tag="ACHKJF_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP0950233.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21332..22624)
                     /gene="tolB"
                     /locus_tag="ACHKJF_002910"
     CDS             complement(21332..22624)
                     /gene="tolB"
                     /locus_tag="ACHKJF_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP0950234.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22757..23920)
                     /gene="tolA"
                     /locus_tag="ACHKJF_002911"
     CDS             complement(22757..23920)
                     /gene="tolA"
                     /locus_tag="ACHKJF_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0950235.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAEAAEAAAAEARKKAATEAAEKAKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEK
                     AAAEKAAADKKAAAEKAAADKKAAAAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKT
                     GGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKTCTLRIK
                     LAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(23985..24413)
                     /gene="tolR"
                     /locus_tag="ACHKJF_002912"
     CDS             complement(23985..24413)
                     /gene="tolR"
                     /locus_tag="ACHKJF_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP0950236.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24417..25109)
                     /gene="tolQ"
                     /locus_tag="ACHKJF_002913"
     CDS             complement(24417..25109)
                     /gene="tolQ"
                     /locus_tag="ACHKJF_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP0950237.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25106..25510)
                     /gene="ybgC"
                     /locus_tag="ACHKJF_002914"
     CDS             complement(25106..25510)
                     /gene="ybgC"
                     /locus_tag="ACHKJF_002914"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0950238.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
BASE COUNT         6291 a         6748 c         6315 g         6273 t
ORIGIN      
        1 gctaaatgca gttaaaattt ttaacggcca gccacccaaa atcgtcgctt ccattttccg
       61 gagtaataat gaagcatgaa ttatcaagta tgaaggcatt tgtcatactg gcagagtcca
      121 gttcatttaa caatgccgct aaattactca atattacgca acctgcatta acgcgaagaa
      181 taaaaaagat ggaggaagat ttacatattc aactatttga gcgtacaact cgcaaggtta
      241 cgttaacaaa agcagggaaa aggttgctcc cggaggcgcg ggaattaata aagaaattcg
      301 atgaaacgct ttttaatatt cgtgatatga atgcttatca tcgtggtatg gtgacgttag
      361 cgtgtattcc gaccgcagta ttttattttt taccgctggc aatcggtaaa tttaatgagc
      421 tgtatcccaa tattaaagtg cggattctgg aacaaggcac gaataattgc atggaatcgg
      481 tgctgtgtaa cgaatcagac tttggcataa acatgaacaa cgtcacaaat tcatccattg
      541 attttacccc actggtcaac gagccattcg tgctggcctg tcggcgtgac catccattgg
      601 ccaaaaagca gcttgtagaa tggcaggagt tagtgggtta caaaatgatt ggcgtacgtt
      661 cctccagcgg caaccgattg ttgatagaac aacagctagc cgacaagccc tggaagctgg
      721 actggtttta cgaagtgcgt catctttcga cgtcattagg actagtggag gcaggactgg
      781 ggatttcggc gcttcctggt ctggcaatgc cccatgcgcc ctattcttcg attattggta
      841 tcccactggt ggaacccgtt atacgtcgga cattgggtat tattcgccgc aaagatgccg
      901 tgctttctcc ggcagctgaa cgcttttttg ccttgctgat taatttgtgg actgacgata
      961 aagacaattt gtggaccaat atcgtcgaac gccagagaca tgcgttacag gagataagtt
     1021 aatacaccgg cgcaggagag actgcgccgg ttaaatcagc ggttagtgtg cgttaaccac
     1081 cacccacatt ggtccctgcc cgaccgcata acggcctttt tcatgcagta gtccctgctc
     1141 gccaacaatt tcgtataccg agatgtggtg agatttttgc ccggcggcaa tcagatactt
     1201 gccgctgtga tcaacattga agccgcgcgg ctgggtttcc gttggctgga agccttcttt
     1261 actcaacacg ctgccatctt ccgaaacgct gaaaacggta atcaggctgg cggtacggtc
     1321 gcaggcgtat aaatggcgac catccggggt gatatgaata tcagccgccc aacgggtgtc
     1381 ggagaagttt tccggcatca tatccagcgt ctggacacat tcgatattac cgtgcggatc
     1441 tttcagttcc cagacatcca ctgagctgtt taactcattg acgcaatacg catattgttc
     1501 gtttggatgg aataccatat gacgcgggcc ggccccttca acggtggtca cttccgcagg
     1561 gtcctgcgcc acgagatgac catcatcgct gaccgtaaac aggcaaatgc gatcctgctt
     1621 taatgccgga acccacagcg tacggtcgtc cggtgagata ttggcggaat ggcaaccgtc
     1681 cagcccctcg accacatcga cgacgcccac tggcaggcca tcttccagac gcgttacgct
     1741 cacgttaccc gcattgtaag aacctacaaa gacaaactgc ccctggtgat cggtggaaat
     1801 atgcgtcgga ctacccggca gcgcagactc tgcggcaaag gtcagtgcgc catcgtccgg
     1861 ggcaatacga tacgccagga cgcgaaactc agggcgaaca ccaacataga gataacgttt
     1921 gtccgggctg accaccatcg gctgcacctg ccccggcaca tcgacaacct gtgtcagcgt
     1981 cagtgcgcct tcatgattca ggttccagac gtgaatttgc tggctctcag ggctggcgat
     2041 ataaactgtt tgcttcatga atgctccttt gcatttagca actgactgta acagctaaaa
     2101 ttagtcgctt ttggcggtga atgctgaatt aaaacgcaga attttgtccg atagtggtat
     2161 cggtgtacca ttcgcgcaga cgaaaattct taaccttaat ctggaatacg ccatgaccac
     2221 acgcgtgatt gctctcgact tagacggcac cttattgacc ccgaaaaaga ccctgcttcc
     2281 ttcatcgata gaagccctgg cccgcgctcg cgaagcaggc tatcaattaa tcatcgtcac
     2341 aggtcgccat cacgtcgcta ttcatccttt ttatcaggcg ctggcgctgg atacacctgc
     2401 tatttgctgt aatggcacct atttgtatga ttatcatgca aaaaccgtgc tggaagcgga
     2461 cccaatgccc gttaataaag ccctgcaact cattgagatg ctgaatgaac accacattca
     2521 cggtctgatg tatgtcgatg atgcaatggt ctatgagcac ccgaccgggc atgtcattcg
     2581 cacgtctaac tgggcgcaaa ccctgccgcc ggaacagcgt ccgactttca cacaagtcgc
     2641 ttctctggct gaaacggcgc aacaagttaa cgccgtatgg aagttcgccc tgacgcacga
     2701 tgacctgccg caattgcagc attttggtaa gcatgtcgaa catgaactgg gactggagtg
     2761 tgaatggtcc tggcacgatc aggttgatat tgcacgcggc ggcaacagca aaggtaaacg
     2821 tttgacgaaa tgggttgagg cgcaaggttg gtcgatggaa aacgtcgtgg cattcggcga
     2881 taactttaat gatatcagta tgctggaagc cgctggtaca ggcgtggcga tgggcaacgc
     2941 cgatgacgcg gtaaaagcgc gcgccaacat tgtgattggt gataacacca ccgacagcat
     3001 tgcccagttc atttatagcc acctgattta atcaggcggt tatcgacaca cttttaattt
     3061 gcgcgtacag ccacaggcca ggtttgatcg ccagttcatc cctggcccac gggctgatac
     3121 gcgcccacag cgttttaccg ccgacttcca gctccacttc cacctggccg ttgtcgtcat
     3181 aactattaac aacttttgcc cgcaatacgt tacgaatgct ggtttgctgc ggcggttgta
     3241 aaaccagaga aacatcggaa gcctgaatgc ggatacgtag cgcagcttgc agcggttcgt
     3301 ccagcttatt gacccacaaa tgctgatcgc ccagcgccag cgcagtcatc gcgtaatgcg
     3361 gatgatgctc aagtaccgtc actttcagaa tgctactttg ttgctctttc ggcagccacg
     3421 gattcatcac gctactaccc cagacttctt ccagcgcgcc aaaggctttc acctgaccgt
     3481 tttccagtac catcactctg tctgccagat ggaggatctc atccagcgaa tggctgacat
     3541 acaacatcgg aatgttgatt tcccgcgtca gacgttgcag ataaggcaac agttcgcgtt
     3601 tacgcggaat atccagtgac gccagcggtt catccagcaa cagcaattcc ggagccgtca
     3661 gcaaagcccg accaatcgcc acgcgctgtt tttcgcctcc ggacaggctg cccggtaaac
     3721 ggtcaagcaa cggttcaatg cctaaaagcg ccaccagctt atcgaactga tcgaccatgc
     3781 ttttcgccat gccgtagcgc aaattgccac gcactttgta atgcgggaac agccgtgcat
     3841 cctgaaaaac atagccaacg cgacgttttt ctggcgtcag gcagatacct ttttcggcat
     3901 catttagtac ccgaccattg aggacaatcc tccctttttg cgggcgcgtc agtccactga
     3961 tggcgttaat cagcgaagtt tttccggcac cggagacgcc aaagatagca gtgatgccat
     4021 tggcgggcag cgtttcatta atagtcaggc aatggttgcc caaggtctgg gaaaaattca
     4081 gttccagcat gattagcgcc ccgcccgttc acggctgatt ctggccagcc attctgaaat
     4141 caacagggag atcatcgcca gcgcaataga aataatgcac agtctcgctg cgccactttc
     4201 cccgccgggg gtctgaatca gggtatacat ggcagaagga atggttcgcg tttcaccagg
     4261 aatgttcgac acaaaggtga tggttgcgcc aaattcaccg agagaacgag caaaagccag
     4321 taccgtacca acaataattc ccggtaaggt cagcggtaac gtgatagtaa agaaaacgcg
     4381 ccagcgcccg gcccccagtg ttcttgcggc ctgttccagt ttgacatcaa ccccttccag
     4441 cgccagacga attgcccgca ccattagcgg aaacgacatg acggcggcag cgagaaccgc
     4501 gccgcgccag ctaaaggcga aggtaatacc aaaccagtca tacagacgtt caccgataaa
     4561 tccgcgccgt cccatcgaaa ctaataataa gtaaccgacg accacgggcg gtaacaccag
     4621 cggtagatgc agtacgctgt cgagcaaagc tttgcccgga aacgtgcaac gcaccagtaa
     4681 ccaggcaaaa aagatcccaa acggcaggct aaacagcaca gccagggaag aaactttcag
     4741 gcttaataaa actgcctgcc attctggatc ggtcagtatc attacttggt tgtaaatccg
     4801 taacgtttaa agatttccgc agcctgcggt cccttcagat aatcataaaa tgctttcact
     4861 gtcgcattgt tatgcccttc cacaacagca accggatatt ccactttttt atgtgaatct
     4921 tccgggaagg tggcaaccac ttttacccct ttgctggcaa ctgcgtcaga accgtaaaca
     4981 atgcccaacg gcgcttcatt gcgttcgacc aacgccagcg cgccacgaac atcttccgcc
     5041 ggagccagtt tcggggagag cgtatcccat gcgcccagtt tttgcagtgc ttcttttgca
     5101 taaatgccag cagggacatg ttccggatcg ccaaccgcga ggcgaccgcc attcagcagc
     5161 gaagtccagt tagttttgct gtcgatgatg aaatctttct gctcgctggc tttcggtgct
     5221 acaacgacca ggctattgcc gagcagtgtc tgacgcgtag ctgtatcgat cgctttttta
     5281 tcaactgcat aatccatcca tttctgatcg gcagaaataa acagatccgc aggcgcaccc
     5341 gcttcaatct gacgggcgag agtagatgac gaagcgaaag aagaaaccac atccacgcct
     5401 ttctcttttt tatactgcgt agcaatgtcc tgcattgcgt tagtcagtga tgcggcggcg
     5461 aacaccgtga ttttcccttc atctgccagt gcattgccag caacagcgaa agagagtgcc
     5521 gccccggcaa acaagttcaa ccatttacga gccattggta accccttaat gtaacgttat
     5581 gtaggcgaca atataacgac taactcggaa ttttcccagc agttattact aacctttgtg
     5641 aggtagataa gaaaaaatat cggcaggaaa agcaggaagt tgagaaaaag aaaatgcccg
     5701 actaagcggg cattcaggga atcaatgatt ttgtccgggc tggtcttttt taccaacacc
     5761 agaaaagatg ttgaatactt caccaagacc gtaaatcaga cccaggatga tggccatcac
     5821 gacaggtacc atgattacgg cgaataccag actttttaat aactctaaca tggtcaactc
     5881 cagatatagt catgagacta ttctaaccgc taagcacaga aaagcactcc ccttttgtgc
     5941 ggtcagcttt gcgtggcgtt cgttttccgt cacaataaga cttttgccag gacattgtta
     6001 tgcaggccga aatccttctc acccttaagc tccaacaaaa attattcgcc gacccgcgcc
     6061 gcatttcgct actaaaacac attgcgcttt ccggttccat tagccaggga gcgaaagatg
     6121 ccggtatcag ctataaaagc gcctgggatg ccattaacga gatgaatcag ttaagtgagc
     6181 atattctggt cgagcgcgca acaggcggta aaggtggcgg cggcgcagta ctgacccgct
     6241 atggtcagcg actgattcag ctctatgact tactggcgca aatccagcaa aaagcctttg
     6301 atgtgttaag tgacgatgac gccctgccgc tgaacagcct gctggccgcg atctcacgtt
     6361 tttcactgca aaccagcgcc cgtaaccagt ggttcggcac catcaccgcc cgcgatcatg
     6421 atgacgttca acagcatgtt gatgtcttac tggctgacgg aaaaacacgc ctgaaagtcg
     6481 caattaccgc acaaagcggc gcacgtctgg ggctggatga aggcaaagaa gtgttgatat
     6541 tgctaaaagc gccgtgggta ggcattactc aggacgaagc ggtcgcacaa aacgccgaca
     6601 accaattacc gggtattatt agtcatattg agcgcggcgc agagcagtgc gaagtattaa
     6661 tggcgctacc cgacgggcaa acactgtgcg ccacagtgcc ggttaatgaa gcgacttctc
     6721 ttcagcaagg acagaatgtc acggcctact ttaatgccga cagcgtgatt atcgccacgc
     6781 tatgctaagc gtgttgacaa tttgttatga aacacgtatc cctatcagta atcgctgcac
     6841 aaaatgggat ataaaatgtc atcgttgcaa attttgcaag gcacgtttcg tcttagcgac
     6901 acaaaaacgc tgcaattgcc tcagctaacg ttaaacgcgg gtgatagttg ggcgtttgtc
     6961 ggttcgaatg gaagtggaaa gtcggcgctg gcccgtgcgc tggcggggga acttccgctt
     7021 ttgaaaggtg aacggcaaag ccagttttcc cacatcactc gtctctcctt cgagcaattg
     7081 caaaagctcg tcagcgacga atggcagcgg aataacaccg atatgctcgg ccctggcgaa
     7141 gatgacaccg gacgcactac ggctgagatc attcaggatg aagttaagga tgcaccgcgt
     7201 tgcatgcaac tggcgcagca gttcggtatt accgccctcc tcgaccgact ctttaaatac
     7261 ctttccactg gcgagacgcg aaaaaccctg ctgtgtcagg cgctgatgtc ggagcctgac
     7321 ttgttgattc ttgatgagcc gttcgatggc ctggatgttg cctcacgtca gcagctggct
     7381 gagcgactcg cctcgttaca tcagtccggt attactctgg tactggtgct caatcgcttc
     7441 gatgagatcc cggaatttgt ccagtttgct ggcgtgctgg cggattgtac gttagcggaa
     7501 acgggcgcta aagaggaact gctccagcaa gcactcgtcg cgcaactggc gcatagcgaa
     7561 cagcttgaag gtgtgcaact gccggagccg gatgaacctt cagcacgtca cgccttaccc
     7621 accaacgaac cgcgcattgt gctgaataat ggcgtggttt cttataacga tcgccccatt
     7681 cttaataacc ttagctggca ggtgaatcct ggcgagcact ggcaaattgt cgggccaaat
     7741 ggtgcgggaa aatcgacgtt attaagcctg attactggcg atcatccgca aggttacagc
     7801 aacgatttga cgcttttcgg ccgacgtcgc ggcagcggcg aaaccatctg ggatatcaaa
     7861 aagcatatcg gttacgtcag cagtagtttg catctggatt accgggtcag cactaccgtg
     7921 cgtaatgtga ttctttctgg ctattttgat tcgattggca tttatcaggc cgtttcggat
     7981 cgccagcaaa aactggtgca gcagtggctg gatattctcg gcattgataa acgcacggct
     8041 gacgttccgt tccatagtct ttcctgggga cagcagcgtc tggcgctgat cgtccgcgcg
     8101 ctggtgaaac atccgacatt gcttattctc gatgaaccac tacaggggct tgatccgctc
     8161 aatcgccaac ttatccgccg ttttgttgat gtgctgatta gcgaaggtga aacgcaattg
     8221 ttgtttgttt cgcaccacgc tgaagatgcg cctgcctgta ttacccatcg tcttgagttc
     8281 gtgccggacg gtgaccttta tcgctatgcg ctgacaaaaa taaactgagt tggtaatgct
     8341 gactgtgccg gatgtggcgt aagccccctc tccggcctac agttcgacgc atgcaggcat
     8401 gaaaccgcgt cttttttcag ataaaaagcg caatcattca taaaccctct gttttataat
     8461 cacttaatcg cgcataaaaa acggctaaat tcttgtgtaa acgattccac taatttattc
     8521 catgtcacac ttttcgcatc tttgttatgc tatggttatt tcataccata agcctaatgg
     8581 agcgaattat gagagttctg gttaccggtg gtagcggtta cattggaagt catacctgtg
     8641 tgcaattact gcaaaacggt catgatgtca tcattcttga taacctctgt aacagtaagc
     8701 gcagcgtact gcctgttatc gagcgtttag gcggcaaaca tccaacgttt gttgaaggcg
     8761 atattcgtaa cgaagcgttg atgaccgaga tcctgcacga tcacgctatc gacaccgtga
     8821 tccacttcgc cgggctgaaa gccgttggcg aatcggtaca aaaaccgctg gaatattacg
     8881 acaacaatgt caacggcact ctgcgcctga ttagcgccat gcgcgccgct aacgtcaaaa
     8941 actttatttt tagctcctcc gccaccgttt atggcgatca gcccaaaatt ccatacgttg
     9001 aaagcttccc gaccggcaca ccgcaaagcc cttatggcaa aagcaaattg atggtggaac
     9061 agatcctcac cgacctgcaa aaagcccagc cggactggag cattgccctg ctgcgctact
     9121 tcaacccggt tggcgcgcat ccgtcgggcg atatgggtga agatccgcaa ggcattccga
     9181 ataacctgat gccatacatc gcccaggttg ctgtaggccg tcgcgactcg ctggcgattt
     9241 ttggtaacga ttatccgacc gaagatggta ctggcgtacg cgattacatc cacgtaatgg
     9301 atctggcgga cggtcacgtc gtggcgatgg aaaaactggc gaacaagcca ggcgtacaca
     9361 tctacaacct cggcgctggc gtaggcagca gcgtgctgga cgtggttaat gccttcagca
     9421 aagcctgcgg caaaccggtt aactatcatt ttgcaccgcg tcgcgagggc gaccttccgg
     9481 cctactgggc ggacgccagc aaagccgatc gcgaactgaa ctggcgcgta acgcgcacac
     9541 tcgatgaaat ggcgcaggac acctggcact ggcagtcacg ccatccacag ggatatcccg
     9601 attaaggaac gaccatgacg caatttaatc ccgttgatca tccacatcgc cgctacaacc
     9661 cgctcaccgg gcaatggatt ctggtttcac cgcaccgcgc taagcgcccc tggcaggggg
     9721 cgcaggaaac gccagccaaa caggtgttac ctgcgcacga tccagattgc ttcctctgcg
     9781 caggtaatgt gcgggtgaca ggcgataaaa accccgatta caccgggact tacgttttca
     9841 ctaatgattt tgcggccttg atgtctgaca cgccagatgc gccagaaagc aacgatcctt
     9901 tgatgcgttg ccagagcgcg cgcggtacca gccgggtgat ctgcttttca ccggatcaca
     9961 gtaaaacgtt gccagaactg agcgttgcgg cattgacgga aatcgtcaaa acctggcagg
    10021 agcaaaccgc agagctggga aaaacgtacc cgtgggtgca ggtctttgaa aacaaaggtg
    10081 cggcgatggg ctgctctaac ccgcatccgc acggacaggt ttgggcaaat agcttcctgc
    10141 ctaacgaagc tgagcgcgaa gaccgcctgc aaaaagaata ttttgccggg cagaaatcgc
    10201 caatgctggt ggattatgtt cagcgcgagc tggcagacgg tagccgtacc gttgtcgaaa
    10261 ccgaacactg gttagccgta gtgccttact gggctgcctg gccgttcgaa acgctactgc
    10321 tgcccaaagc ccacgtttta cggatcaccg atttgaccga tgcccagcgc agcgatttag
    10381 cactggcgtt gaaaaagctg accagtcgtt atgacaacct cttccagtgc tccttcccct
    10441 actctatggg ctggcacggc gcgccattta atggcgaaga gaatcaacac tggcagctgc
    10501 acgcgcactt ttatccgcct ctgttgcgct ccgccaccgt acgtaaattt atggttggtt
    10561 atgaaatgct ggcagagacc cagcgagacc tgaccgcaga acaggcagca gagcgtttgc
    10621 gcgcagtcag cgatatccat tttcgcgaat ccggagtgta agaaatgagt ctgaaagaaa
    10681 aaacacaatc tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc
    10741 ctggccgcgt gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct
    10801 gcgcgattga ttatcaaacc gtgatcagct gtgcgccacg cgatgaccgt aaagttcgcg
    10861 taatggcagc cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg
    10921 cgcatgaaaa ctatcaatgg gcgaactacg ttcgtggcgt ggtgaaacat ctgcaactgc
    10981 gtaacaacag cttcggcggt gtggacatgg tgatcagcgg caatgtgccg cagggggccg
    11041 ggttaagttc ttccgcttca ctggaagtcg tggtcggaac cgtattgcag cagctttatc
    11101 atctgccgct ggacggcgca caaatcgcgc ttaatggtca ggaagcagaa aaccagtttg
    11161 ttggctgtaa ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg
    11221 ccttgctgat tgactgccgc tcactgggga ccaaagcagt ttccatgccg aaaggcgtag
    11281 ctgtggtcat catcaacagt aacttcaaac gtaccctggt tggcagtgag tacaatactc
    11341 gccgtgagca gtgcgaaacc ggtgcgcgtt tcttccagca gccagctctg cgcgatgtca
    11401 ccattgaaga gttcaacgct gttgcgcatg aactggaccc gatcgtggcg aaacgcgtgc
    11461 gtcatatcct gaccgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg
    11521 acctgaaacg tatgggcgag ttaatggcgg agtctcatgc ctctatgcgc gatgatttcg
    11581 aaatcaccgt gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggtgaca
    11641 aaggtggcgt acgcatgacc ggcggcggat ttggtggctg tatcgtcgcg ttgatcccgg
    11701 aagaactggt gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta
    11761 ttaaagagac tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac
    11821 tcccgcactg gcacccgatg gtcagccgta ccgactgtta actttgcgta acaacgcagg
    11881 gatggtagtc acgctgatgg actggggtgc gactttactt tccgcccgta ttccgctttc
    11941 cgatggcagc gtccgcgagg cgctgctcgg ctgtgccagc ccggaatgct atcaggatca
    12001 ggctgcgttt ctgggggcct ctattggtcg ttatgccaac cgcatcgcca atagccgtta
    12061 tacctttgac ggtgaaaccg tgacgctttc gccaagtcag ggcgttaacc agctgcacgg
    12121 cggcccggaa gggttcgaca aacgtcgctg gcagattgtg aaccagaacg atcgtcaggt
    12181 gctgtttgcc ctgagttcag atgatggcga tcagggcttc ccgggtaatc tcggcgcgac
    12241 ggtgcaatat cgtctgaccg acgataaccg tatctctatt acttatcgcg ccacagttga
    12301 taaaccttgc ccggtgaata tgactaatca cgtctatttc aatcttgacg gcgagcagtc
    12361 tgacgtgcgc aatcacaagt tgcagattct ggcggacgaa tatctgccgg ttgatgaagg
    12421 cggcattccg cacgacggcc tgaaatctgt cgccggaacg tcttttgatt tccgtagcgc
    12481 caaaatcatc gccagtgagt ttcttgccga cgacgatcag cgcaaagtga aaggttacga
    12541 tcacgcattc ttgttacagg ccaaaggcga tggcaagaaa gtggcggcgc atgtctggtc
    12601 agcagatgaa aaattgcagc tgaaggtcta caccaccgct ccggctctgc aattctactc
    12661 cggcaacttc ctcggcggca cgccatcaag aggaactgaa ccttacgccg actggcaagg
    12721 cctggcactg gaaagcgagt ttctgccgga cagcccaaac caccctgaat ggccgcaacc
    12781 ggactgcttc ctgcgtcctg gcgaagagta ttccagcctg acggaatatc agtttattgc
    12841 tgagtagcac ctgaagccct cgtaatgagg gctttatcct tatattgctc aaatcaccag
    12901 caaacaccga catatttgca actcaatatt cacaacaacc ttacactgcg acactatttt
    12961 cgctatggtt atgcgtaagc attgctgttg cttcgtcacg gcaatataat gagaattatt
    13021 atcattaaaa aatgatttga ggagtaagta tatggctgta actaagctgg ttctggttcg
    13081 tcatggcgaa agtcagtgga acaaagaaaa ccgtttcacc ggttggtacg acgtggatct
    13141 gtctgagaaa ggcgtaagcg aagcaaaagc agcaggtaag ctgctgaaag aggaaggtta
    13201 cagctttgac tttgcttaca cctctgtgct gaaacgcgct atccataccc tgtggaatgt
    13261 gctggacgaa ctggatcagg catggctgcc cgttgagaaa tcctggaaac tgaacgaacg
    13321 tcactacggt gcgttgcagg gtctgaacaa agcggaaact gctgaaaagt atggcgacga
    13381 gcaggtgaaa cagtggcgtc gtggttttgc agtgactccg ccggaactga ctaaagatga
    13441 tgagcgttat ccgggtcacg atccgcgtta cgcgaaactg agcgaaaaag aactgccgct
    13501 gacggaaagc ctggcgctga ccattgaccg cgtgatccct tactggaatg aaactattct
    13561 gccgcgtatg aagagcggtg agcgcgtgat catcgctgca cacggtaact ctttacgtgc
    13621 gctggtgaaa tatcttgata acatgagcga agaagagatt cttgagctta atatcccgac
    13681 tggcgtgccg ctggtgtatg agttcgacga gaatttcaaa ccgctgaaac gctattatct
    13741 gggtaatgct gacgagatcg cagcgaaagc agcggcggtt gcaaaccagg gtaaagcgaa
    13801 gtaaacgtca ttcgtttaaa atgagaaagc cgactgcaag tgagtcggca tttttgttgc
    13861 taacaatgga gcacattgcc tgatgcgacg ctgcgcgact tatcaggcct gtggtgattc
    13921 atcggatacg ccactctgac ggcgcatccg acaattaaac cttacccgcg acgcgctttt
    13981 actgcattcg ccagttgacg taacagagca tcggtatctt cccagccgat gcaggcatcg
    14041 gtgatgctct taccgtaggc cagcggctcc ccgctctcga ggctctgatt gccttccacc
    14101 agatggcttt ccaccatcac gccaataatg gccttttcgc caccggcaat ctgctggcaa
    14161 acgtcagcac aaacatccat ctgctttttg aattgtttgg acgagttagc atggctgaaa
    14221 tcgatcatca cctgtgctgg caggcctgct ttgttcagcc cttctttcac ttcagcaacg
    14281 tgcttcgcgc tgtagttagg ctctttaccg ccgcgcagaa tgatatggca atcgccgtta
    14341 ccgctggtat tcacaatcgc cgaatgcccc catttcgtta cggacaggaa gcagtgcggc
    14401 gcaccggcgg cattaatggc atcgatagcc actttaatcg taccgtcggt gccatttttg
    14461 aagccgaccg gacaagaaag ccctgatgcc agttcgcggt gcacctgcga ttcggtggta
    14521 cgtgcgccaa ttgcgcccca gctcatcagg tcagcgagat attgtggggt gatcatatcg
    14581 agaaactcac ctgccgctgg cagaccgctg tcgttaatat caagcagcaa tttacgggct
    14641 atacgcagac cgtcgttgat ctggaagcta ttatccatat gcggatcgtt aatcagccct
    14701 ttccagccca ccgtggtacg cggcttttca aaatagacgc gcattacgat ttctagctca
    14761 tctttcagct cttcacgcag cgccagcaag cgagtggcat actcttttgc cgcgacagga
    14821 tcatgaattg agcatgggcc aatcacaacc aacaggcgat catcattacc tttcaggatc
    14881 ttatggatcg cttttcgggc atgggcaacc gtattcgcgg cattttcagt agcggggaat
    14941 ttttccagca atgcgacagg aggaagtaac tctttgattt ctttgatgcg taaatcgtcg
    15001 ttctgataat tcatgtctgt tccagtgttg ccatacttat cttagtgaat gcaatacttc
    15061 caatctatat cttccgtcag aatgtgtaaa cggggtttta cactatgaac ggattaatcc
    15121 tggaattagc aagaaaaacg ccagattgtc gcgaaaaacg agatctctcc tacaatttct
    15181 aactgtaact cctttgtttg ttaattattt caagattctc tgctgcgttt cataacctgg
    15241 ctgaaatctt aaaccaatgc cttatattca cctgcaaatg cactgttgga agaggttatc
    15301 cgacataacg accataacag gagcatccta tgaaaatgac aaaactggcc acattatttc
    15361 tgactgccac tctaagcctt gccagcggtg ccgcactggc tgccgatagc ggagcgcaaa
    15421 ctaataacgg ccaggcaaac gccgcagctg atgcgggcca ggtagcccct gacgcccgtg
    15481 aaaatgtcgc gccaaataac gtcgacaata acggggtaaa taccggttct ggtggcgcaa
    15541 tgctgcattc ggatggttct tcaatgaaca atgacggaat gaccaaagat gaagagcaca
    15601 agaacaccat gtgcaaagat ggtcgctgtc cggatatcaa taaaaaggta caaaccggtg
    15661 atggcatcaa taatgatgtc gataccaaaa ccgacggtac cacgcagtaa ccctgcaaaa
    15721 tagccaataa tgggagagcg gagtctctcc cttttaattt ttcacttcta aaagcagtat
    15781 gatgtcataa agttaagtga gaataattaa gggatgactc gtcatggcgc actcacactc
    15841 acacacgtcc tcacacctgc cagaagataa taatgctcgt cgcttgttgt atgctttcgg
    15901 cgtgactgcc gggtttatgc tggtagaagt cgttggtggt tttctttctg gttctctggc
    15961 attgctggcc gatgcgggtc atatgttgac cgatactgcc gccctgcttt tcgccctact
    16021 cgccgtgcaa ttttcacgtc gtcctcccac cattcgccac actttcggtt ggctaagact
    16081 gaccacgctc gcggcgtttg tgaatgccat agccctcgtg gtaattacca ttttgattgt
    16141 ctgggaggcg atagaacgct tccgcacgcc gcgtccggtc gagggcggca tgatgatggc
    16201 aattgccgtg gctgggttgc tggcaaatat actttctttc tggttacttc atcacggcag
    16261 cgaagagaaa aacctcaacg tacgagcagc ggcactgcat gtgctcgggg acctgctggg
    16321 ttcggttggg gctattattg ccgcgttgat tattatctgg actggctgga cacctgctga
    16381 ccccattctc tcgatactgg tgtcgcttct ggttctgcgt agcgcgtggc gactgttgaa
    16441 agatagcgtg aatgaattac ttgaaggtgc accggtatcg ctggatattg ctgaactgaa
    16501 gcgtcgtatg tgccgggaaa tcccggaagt ccgcaatgtg caccatgtac atgtatggat
    16561 ggtaggcgag aagccggtga tgacgttgca tgtgcaggtg atcccgccac acgatcacga
    16621 tgccttgttg gatcagatcc aacactacct gatggatcac tatcagattg agcacgccac
    16681 cattcagatg gaatatcaac cttgtcatgg gccagactgc cacctgaatg aaggggtgtc
    16741 tggccattca catcatcacc attaatggga cagcgcgcgt gagcctcttt cacgtgcgct
    16801 gttgatccac atccggctgc cgttgagcgc aataaaggtc aggatgatgt actccagaga
    16861 catggcgtaa acgccctgaa gtgcaaaaat aacgacgcta atcacgttaa taatcaccca
    16921 caacagccag ttttccacat acttacgcgt catcagaatc attgccacga tagataacac
    16981 catcatgcat gaatcccaga acgggaaagc gtccggttgc agttcaggca tcacaacctg
    17041 taatcctaat gcttgcatga tcatgactgc cacgcgggtc aaaaatgcaa acaccggatt
    17101 gataaatacc gtcatcagac caatcgaaac aacgcaaacc gccaaccagc tgagtgcctt
    17161 cggcaatggc aaccagcgaa ttttcaactc cgcctcgttc tgactggttt gtcgcgacca
    17221 cgcataccaa ccgtaaatat tcgcggcaaa gaaaaacacc tgtaatagca ggctggcata
    17281 cagctgaatc tgaaagaaaa taatgccaaa caaggtgacg ttgatcaggc caaagaagta
    17341 gttgctgatc ttctccagac tggcaaggcc aatacacagc aacccggcga tcgtgcctac
    17401 cgcttcgatc catgagagat cataaccgcc tgcccctatt ggtatatgta ccaggatact
    17461 ctgcacacta aaaaaatcca tctttccccc aaagcgtaac gttacgcctt attattcgtt
    17521 atccacgtag tgtagccgca aaatccagca tacgattgag cggcaccagc gccctctctc
    17581 gcagcctttc atcaacatga acctcgtgat tgcttccttc ctgttctaat gcctctgcga
    17641 tggactgaag gccattcatg gccatccacg gacaatgcgc gcagctgcgg caggttgcac
    17701 cctcacctgc ggttggtgct tccagtaact ctttatctgg cactgcctgc tgcattttgt
    17761 agaaaatgcc ccgatcggtt gccacgataa gccgctgatg tggcaatgtt ttcgcagcag
    17821 cgatcagttg actggtggaa ccgaccgcat ccgccatatc gacaatagct tgtggtgatt
    17881 ctggatgcac cagtatggca gcatccgggt attcttcttg caagcgggtt aacgcctgag
    17941 tcttaaattc atcatgcaca atacaggcac cctgccagca tagaatgtct ccacccgtct
    18001 gtttttgcac gtaacgcccc agatgtttgt cgggtgccca gatgattttt tcacccaaac
    18061 tatcaagatg atcaataagt tcgacggcaa tgcttgaagt taccacccaa tctgcgcgcg
    18121 cttttaccgc agcagaagtg ttggcgtaga cgacgacagt acgatcggga tgggcatcgc
    18181 aaaatgcgtt aaattcttca acagggcagc cgagatccag tgaacattca gcctgaagtg
    18241 tcggcatcag aattgttttt tccggactga gaattttggc ggtttctccc ataaatctca
    18301 ccccagcgac taacaaagta gaagcgggat gctttgcacc gaagcgcgcc atttccagag
    18361 aatcagaaat acagccaccg gtttcttctg ccagttgttg aatttcggga tcggtatagt
    18421 agtgggcaac catcaccgca ttacgttctt ttagcagacg ttttatcttc tcgcggtaat
    18481 acgctttttc atcaatgctt aacggcgtcg gcttcggggg gaaaggataa atcgccgtgt
    18541 ctggatcaaa cattacgctc atcttaccat ctcgttttac aggcttaacg ttaaaaccga
    18601 cattagcgta atattcgctg ttatcggtgt aatttgtttt atatgctaaa caagatagtc
    18661 gaatggtggg gaaaagtcac tcgaattacg cctaattcca ccgacgtaga gttgcttgca
    18721 cagtaagctc tgacgatgtc atcaggttta caaagtgaaa ttgaggatgc ctgaatagca
    18781 aaaaagcgcc tttagggcgc ttttttacat tggtgggtcg tgcaggattc gaacctgcga
    18841 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgaccccttg cgggattatg
    18901 ctgctttaat cattcaggat ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    18961 aaagtcaact gctctaccaa ctgagctaac gacccatacg ggtgttacct gaaagatttc
    19021 tactcggggc caatctttaa gattggtgtg tcgtgcagga tgactcgctt cgcttgccct
    19081 acgggccgtt gctggcgcaa cgttatcctt cacgcttaac atctgagttt gatgttaaaa
    19141 ttggtgggtc gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc
    19201 aactgagcta acgacccgag tggtgggtga tgacgggatc gaaccgccga ccccctcctt
    19261 gtaagggagg tgctctccca gctgagctaa tcacccgata ctgcgctgga tactacaaac
    19321 tacttccacc gaacccaccg taattagtgg tgggtcgtgc aggatgactc gcttcgctcg
    19381 ccctacgggc cgttgctgac gcaacgttat cctacacgct taacatctgt cttcgatgct
    19441 aaaactggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
    19501 taccaactga gctaacgacc cggtggtggg tgatgacggg atcgaaccgc cgaccccctc
    19561 cttgtaaggg aggtgctctc ccagctgagc taatcaccca atactacgct ggatactaca
    19621 actaactatg ttagtggtgg gtcgtgcagg atgactcgac ttcgtctcgc ccttcgggcc
    19681 gttgctgatg caacgttatc cttcacgttt tactaccgct tccaccttag cgattggtgg
    19741 gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct accaactgag
    19801 ctaacgaccc actttttcgt tgctttcggt ttgtttgata tcccgtggca acggcggcat
    19861 atattactga tttcagactt gagcgcaaca aaaatttcga tgcagatcac tcaactgctt
    19921 atgattcgca cgacacgacc agaaataatg cgacttctgg tcgtgtgtta tgcattacat
    19981 cgcgttcaga cgtttttgtg cctgtttagc gccatcagta ccagggtatt tactgataac
    20041 ctgctggtat acggctttcg cttttgcggt gtcacctttg tcctgcatga tgacgccgac
    20101 tttaaacatc gcatcagcag cctttggtga cttcggatag tttttcacta ccgaagcaaa
    20161 atagtacgcc gcatcatctt ttttaccctt gttgtagttt aactgaccca gccaataatt
    20221 ggcgtttggc aggtaagttg aatcagggta atttttgatg aaattctgaa atgccaccat
    20281 tgcgtcatcc tggcgggatt tatcctgcac cagcgcaata gctgcattgt aatccgtgtt
    20341 tgcatcgccg cttttcaccg gcgcgccagc attcgcagta ccagcatcag ctgtcggcgt
    20401 cgttgatgcc gccgcaccgc tttgatcgct gctggttgat tgcgccgctg caccaccgct
    20461 gctgaggctg tcgatctgca acaggatctg cttttgccgc tccacgacct gattcagttg
    20521 atactgattt tcctgaatct gaccacgcag ggaatcaata tcggattgat tatcagaaag
    20581 ttgttgctgg agttgggtta aaagctggct gtgagcatta gaaatacgct caagttgagt
    20641 gacgcggtct tcgaccgagc ctgagccgac actactgatt ggtgcctgag caaaagcggc
    20701 ccagggggcc gctataccaa ccagtaacga cagactcaat agttgatgtc tgaagttact
    20761 gctcatgcaa ttctcttagt aaaccagtac cgcacgacgg tttttggagt atgccgcttc
    20821 gtcatgaccc agtactgcag gtttttcttt accgtaagaa acgatggaga tctggtctgc
    20881 agaaacgcct ttaccctgca ggtacatctt aacggcgttc gcacgacgtt cacccaggga
    20941 gatgttgtat tccggagtac cacgttcgtc cgcgtgacct tctacggtga ctttgtaaga
    21001 cgggttgcta cgcaggaagt ttgcatgtgc atccagcatt tgagcgaagt cagaacggat
    21061 atcgtacttg tccagatcga agtaaacgat gttgttctgc tgcagctgtt gcatttgcag
    21121 acgagcctgc tcttcggaag acatgttgcc gttgccgccg ttcgcatcca taccagtgcc
    21181 ggcacccagc atgccttcgc tgccgtcatt gctggcgttc ttgttggaag aacatgccgc
    21241 aattgccata acaggcagag caatcatcag ccctttcagc actttgttca gttgcatttc
    21301 aatgattcct ttactattca attaattatt atcacagata cggcgaccag gcagggaatt
    21361 tgacctgtcc atcagttgcc ggaagacgcg ctttgaaacg cccatctgta gaaaccaaat
    21421 tcagcacgga tcccatcccc tgagaagagc tgtagattac catagtgccg ttaggtgcca
    21481 gacttggcgt ttcatccagg aacgtggacg acagaacttg tacgcctccc gttgccagat
    21541 cttgtttggc aatgtgctgc tgcccaccat tggagctgac cattaccata aatttaccgt
    21601 cgctgctgac atccgcatcc tggttctgcg aaccttccca ggtaatacgt tgtggcgcac
    21661 cgccgttgat attcacttta taaacctgcg gacgaccggc ctggtcagaa gtaaatgcca
    21721 ggttctggct atccgggaac caggtcggtt cggtattgtt actgcgacca tcagtcacct
    21781 ggcggatctg accagaagcc aaatccatta cgtacaggtt cagactaccg gttttcgaca
    21841 aggcgaatgc cagtttgctg ccgtctggcg agaatgcagg tgcaccgttg tgacgcggga
    21901 atgaagccac ctgacgtaca gcgccatttg ccagcgtctg aataaccagc gcggaacgac
    21961 cgctttcgaa ggtcacataa gccagtttag aaccgtctgg tgaccacgcc ggagacatca
    22021 gcggctgcgg tgaacggtga acgacaaact ggttgtaacc gtcatagtca gatacgcgca
    22081 gttcatacgg gaactgaccg ccgttggtct gaacaacgta ggcaatacga gtacggaacg
    22141 cacctttaat gccggtcagt ttttcaaaca cttcatcact ggcggtatga ccagcataac
    22201 gcagccactg cttgttcact ttgtacgagt tctgagcaag tacagtaccc ggtgcgccgc
    22261 cagtgtcaac aagttgataa gcaacattgt aagaaccatc cggattcgga gtgacctgac
    22321 cgacaactac agcgtcaatg cccagtgcgg accatgcagc tggttgtact tcctgcgcac
    22381 tacccggctg ctgtggcaga cgagcgcgat ctaacggatt aaatttaccg ctgttacgca
    22441 agtcagcagc aacgatgccg ccaatatctt caggtgccgc accaggcccc gcccactgga
    22501 aaggaacaac accaatagga cgaccggaat ctacaccgct gtcgatcaca atgcggactt
    22561 cagcatgcag aactgatgcc cacagtatga gaaaaccaaa tgctactcgt aatgcctgct
    22621 tcatcatatc tcccttatct ggaccgatgg cccacgataa tttagcagaa tgttaacaaa
    22681 ctaaaataca caaaactacc agaaccccgt ggcaaccggt gcctgatgtt gaccgtccga
    22741 acagtcaaca tcgcgattac ggtttgaagt ccaatggcgc gtttttgaac acttcatata
    22801 ctgcctggct tggtggtttc gggatcttcg caagtttagc tgctgccaac gcagcctgac
    22861 aaagtgcggg atcgccacct tcaggtttga tatccagtaa cataccatcg ggtgccagtt
    22921 ttatgcgcag cgtacaggtt ttgcctgcat aggacgatgc gtcatagaac ttactttcga
    22981 tagcagattt aatctgcccg gcatagttat tgatatcggc ccctgatgcg ccattgtttt
    23041 tagtattacc actcccggca ggcgaagcat tgttcccttt cgcccctccc cccgttttcg
    23101 gtgcattctt accagagctt agctcaccga aaatatcatc tgcctctgcg gccgcttttg
    23161 ctgcagcggc tttttcagct gcagcttttg cagctgccgc ttttttgtct gcggcggctt
    23221 tttctgccgc tgctttctta tcagctgctg ccttttcagc agccgctttt tctgctgctt
    23281 ttttgtcggc tgcagctttc tctgctgccg ctttcttatc agctgcagcc ttttcagcag
    23341 ccgctttctt ctcagcttct gctttggctt tttcagcagc ttcagttgcc gctttctttc
    23401 ttgcttcagc tgcagctgct tctgccgctt ccgctttctt cgctgcttct tccgcagctt
    23461 tagcatctgc ttcggccttc gctttagcat ctgccgccgc tttcgctgcc gcctcttcag
    23521 cttgcttctg ctttaactcg gcctgttttg cggcttcttc agcctgcttt ttctgctcct
    23581 gagccgctaa ccgctctttc tcaagttgct tcaggcgttc ctgttcagcc gcttgtttct
    23641 cacgcagttc ttcagcagcc tgctgttcct tcatcttgcg ctgttcatca gaacgcttcg
    23701 cgcttgattc ctggctttgc atgcgtttgt actgctcaac taccgcacct gaatcaacca
    23761 tgacagcgtc gatggacgaa ccaccgccgc ctccggctga agcttctata ttctcatcga
    23821 acgaactcca gatcagcgcc gcaaataaga tgacatgcag cactgctgaa ataattatcg
    23881 cccgcttgag cttgtcgttt tgttcggttg cctttgacac tctcggtttc caaaaactgt
    23941 tcgcctgtta cccgctctct ttcaagcaag ggaaacgcag atgtttagat aggctgcgtc
    24001 attaaaccaa ccgatttcac acccgcacta tgtaacaagt tcagtgcttt aattatttca
    24061 tcgtaaggca catcttttgc gccaccgatc agaaagaccg ttttcgggtt ggccttgaaa
    24121 cggctggaca cttccgccac cacctgctct ggtggtaaac gctccaggcg atctttctca
    24181 accaccacgg tgtactgacc aataccagac acttcaacaa tcactggcgg attatcgtta
    24241 ctgctcaccg cctgtgattc agtagcgtct ggcagatcga cctccacgct ctgggtgatg
    24301 atgggcgctg tcgccataaa gatcagcaac agcaccagca gtacgtccag caacggtaca
    24361 atgttgattt cggacttgag atcgcgacga cctcgtccac gcgctctggc catggcttac
    24421 cccttgttgc tctcgctaac ggtaaacgcc tggcggtgca gaatcgcggt aaactcttcc
    24481 ataaagttgt cgtaattcag ttccagtttg tttacgcgct ggttgaggcg gttataagcc
    24541 attaccgccg gaattgcggc gaacagacca attgcagtcg caatcaacgc ttctgcgata
    24601 ccgggcgcaa ccatttgcag tgttgcttgt tttaccgccc cgagggcgat aaaggcgtgc
    24661 atgatccccc agaccgtacc aaacagacca atatacgggc tgatggagcc aaccgtaccg
    24721 aggaacggaa tgtgcgtttc cagattttca agttcacggt tcatggagat acgcatagca
    24781 cgcgacgccc cttccactac ggcttccggc gcatggctat tggcacgatg caggcgcaca
    24841 aactctttga acccgctgta aaagatttgt tccgaaccag tcagattatc ccgtttcccc
    24901 tggctctctt gatagaggcg agagagttcg attccagacc agaatttatc ttcaaacgct
    24961 tcggcttcgc gcgccgctgc gttaagaata cgggtccgct ggataataat ggcccaagat
    25021 gcgattgaaa aaccaatcaa aatcaacatg ataagtttaa ccagaaggct agccttcagg
    25081 aacaaatcaa ggatattcat gtcagtcact gcttaaactc cgcgacaata gacttgggaa
    25141 gcgcacgagg cttcattttg agtgggtcaa cgcaaacaac cagaacctct gcttcattca
    25201 gcaaagtatt ctcggcgttg acaatacgtt gcgtgaaaac caaagaggtg ccacgcattg
    25261 atgttatttc agtctgtatt tcgagcatat cgtcgagccg cgcaggtgcg taatattcca
    25321 ccgtcatttt acgtaccaca aaggcaacgc gttcagccat cagcgcctgc tgactgaagt
    25381 gatgatgacg cagcatctct gtgcgtgctc tttcataaaa agcgacgtaa ctggcgtggt
    25441 acaccacacc accggcatcg gtatcttcat agtagacgcg aaccggccat cgaaacagcg
    25501 ttgtattcac tttacatccc ggtaatgcaa caaaagttag agcttttaaa cgctgctact
    25561 atacgcgcgc gattgaggtt tgggaatgag ggcaagttaa gggagcgtaa aaatttatgg
    25621 gcctgga
//
LOCUS       DAZGXZ010000068        25053 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000068 DAZGXZ010000000
VERSION     DAZGXZ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25053
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.068"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..458
                     /gene="yhaJ"
                     /locus_tag="ACHKJF_002915"
     CDS             <1..458
                     /gene="yhaJ"
                     /locus_tag="ACHKJF_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP0950239.1"
                     /translation="IVIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSE
                     VTRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMV
                     EKDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
     gene            complement(509..865)
                     /locus_tag="ACHKJF_002916"
     CDS             complement(509..865)
                     /locus_tag="ACHKJF_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0950240.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(1107..1472)
                     /gene="yhaH"
                     /locus_tag="ACHKJF_002917"
     CDS             complement(1107..1472)
                     /gene="yhaH"
                     /locus_tag="ACHKJF_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0950241.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            complement(1765..2751)
                     /gene="yqjG"
                     /locus_tag="ACHKJF_002918"
     CDS             complement(1765..2751)
                     /gene="yqjG"
                     /locus_tag="ACHKJF_002918"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP0950242.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            complement(2821..3213)
                     /gene="yqjF"
                     /locus_tag="ACHKJF_002919"
     CDS             complement(2821..3213)
                     /gene="yqjF"
                     /locus_tag="ACHKJF_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0950243.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            complement(3399..3698)
                     /gene="yqjK"
                     /locus_tag="ACHKJF_002920"
     CDS             complement(3399..3698)
                     /gene="yqjK"
                     /locus_tag="ACHKJF_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP0950244.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            complement(3688..4092)
                     /gene="yqjE"
                     /locus_tag="ACHKJF_002921"
     CDS             complement(3688..4092)
                     /gene="yqjE"
                     /locus_tag="ACHKJF_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0950245.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            complement(4095..4400)
                     /gene="yqjD"
                     /locus_tag="ACHKJF_002922"
     CDS             complement(4095..4400)
                     /gene="yqjD"
                     /locus_tag="ACHKJF_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP0950246.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            complement(4438..4806)
                     /gene="yqjC"
                     /locus_tag="ACHKJF_002923"
     CDS             complement(4438..4806)
                     /gene="yqjC"
                     /locus_tag="ACHKJF_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP0950247.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            complement(4953..5336)
                     /gene="mzrA"
                     /locus_tag="ACHKJF_002924"
     CDS             complement(4953..5336)
                     /gene="mzrA"
                     /locus_tag="ACHKJF_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP0950248.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            complement(5340..6002)
                     /gene="yqjA"
                     /locus_tag="ACHKJF_002925"
     CDS             complement(5340..6002)
                     /gene="yqjA"
                     /locus_tag="ACHKJF_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP0950249.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            complement(6024..6107)
                     /gene="yqiM"
                     /locus_tag="ACHKJF_002926"
     CDS             complement(6024..6107)
                     /gene="yqiM"
                     /locus_tag="ACHKJF_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP0950250.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            complement(6347..7123)
                     /gene="exuR"
                     /locus_tag="ACHKJF_002927"
     CDS             complement(6347..7123)
                     /gene="exuR"
                     /locus_tag="ACHKJF_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP0950251.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            7419..8135
                     /locus_tag="ACHKJF_002928"
     CDS             7419..8135
                     /locus_tag="ACHKJF_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0950252.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLVQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            8165..8665
                     /locus_tag="ACHKJF_002929"
     CDS             8165..8665
                     /locus_tag="ACHKJF_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFP0950253.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            8757..11441
                     /locus_tag="ACHKJF_002930"
     CDS             8757..11441
                     /locus_tag="ACHKJF_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFP0950254.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSYSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKVFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            11438..12526
                     /locus_tag="ACHKJF_002931"
     CDS             11438..12526
                     /locus_tag="ACHKJF_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFP0950255.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(12614..13912)
                     /gene="exuT"
                     /locus_tag="ACHKJF_002932"
     CDS             complement(12614..13912)
                     /gene="exuT"
                     /locus_tag="ACHKJF_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP0950256.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            14395..15807
                     /gene="uxaC"
                     /locus_tag="ACHKJF_002933"
     CDS             14395..15807
                     /gene="uxaC"
                     /locus_tag="ACHKJF_002933"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP0950257.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            15822..17309
                     /gene="uxaA"
                     /locus_tag="ACHKJF_002934"
     CDS             15822..17309
                     /gene="uxaA"
                     /locus_tag="ACHKJF_002934"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP0950258.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            17392..17943
                     /gene="ygjV"
                     /locus_tag="ACHKJF_002935"
     CDS             17392..17943
                     /gene="ygjV"
                     /locus_tag="ACHKJF_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP0950259.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(17948..19192)
                     /gene="sstT"
                     /locus_tag="ACHKJF_002936"
     CDS             complement(17948..19192)
                     /gene="sstT"
                     /locus_tag="ACHKJF_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP0950260.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(19591..20556)
                     /gene="alx"
                     /locus_tag="ACHKJF_002937"
     CDS             complement(19591..20556)
                     /gene="alx"
                     /locus_tag="ACHKJF_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP0950261.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(20839..21825)
                     /gene="ygjR"
                     /locus_tag="ACHKJF_002938"
     CDS             complement(20839..21825)
                     /gene="ygjR"
                     /locus_tag="ACHKJF_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0950262.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(21904..22587)
                     /gene="ygjQ"
                     /locus_tag="ACHKJF_002939"
     CDS             complement(21904..22587)
                     /gene="ygjQ"
                     /locus_tag="ACHKJF_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP0950263.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(22664..23167)
                     /gene="ygjP"
                     /locus_tag="ACHKJF_002940"
     CDS             complement(22664..23167)
                     /gene="ygjP"
                     /locus_tag="ACHKJF_002940"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP0950264.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            23252..24388
                     /gene="rlmG"
                     /locus_tag="ACHKJF_002941"
     CDS             23252..24388
                     /gene="rlmG"
                     /locus_tag="ACHKJF_002941"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP0950265.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            24579..24662
                     /locus_tag="ACHKJF_002942"
                     /pseudo
     CDS             24579..24662
                     /locus_tag="ACHKJF_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            24659..>25053
                     /gene="higA"
                     /locus_tag="ACHKJF_002943"
     CDS             24659..>25053
                     /gene="higA"
                     /locus_tag="ACHKJF_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP0950266.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGIS"
BASE COUNT         6622 a         6151 c         6269 g         6011 t
ORIGIN      
        1 atatcgttat cgcgccggat atgcattttc gttcctcgtc ggagatcaac tcgcgcaagc
       61 tctatacctt aatgaacgtc tacgttgccg cgcctgatca cccgattcat caggagccgg
      121 aaccgttatc tgaagtgacg cgcgtgaaat atcgtggaat tgcggtagcg gataccgctc
      181 gtgagcgccc ggtgttgacc gtacagctgc tggacaaaca gccgcgctta acggtgagca
      241 cgattgaaga taaacgtcag gcattactgg cggggcttgg cgtggcgacg atgccgtatc
      301 cgatggtcga aaaagatatt gcggaagggc ggttgcgtgt cgtcagcccg gaatcgacca
      361 gcgagatcga tattattatg gcctggcgtc gtgacagtat gggggaagcg aaatcctggt
      421 gtctgcggga aattcccaaa ctttttagcg gaaaataata tttgttatta ttaataaaaa
      481 ggggcgaaat gtcgccccaa ttctaagatt aatttgaacc aaacttagga tcgtttccgt
      541 aacgattgct gccagaattg ccttctaaac aggcaaatac aaacagaact aaccagccaa
      601 tgatcggtac cagatagaga agcgcccacg cacctgaacg gtcagtatcg tgcagacgac
      661 gaacacacag agcaatgacc ggaatgattg ttgctgccag gtaaatgaga gaaaggaatg
      721 gaaactctaa acctaaaatc aattgaatga cattgataat agcgccgacg atggcgttaa
      781 tcagagtaaa catccagtac tctttacgac gtgcgcgacc agagaaacca acataatttt
      841 ttaatacggc taaataccac tgcataaaaa catcccttta attaaaagtg acgaaagtct
      901 attccgggta ttgtgtaaat gctattcgta tctttctttt tcggatgtta taaaggtttt
      961 gatatttaag ataaatacat ggttgttttt tataggtaat ttatataacg acattgaaat
     1021 attttaaata aacattagta aattattcat tcatcaacat gatgataaaa gccggatgag
     1081 tcagacatcc ggcgagcaga tttgttttaa ggttccagtt tcggatctgg tccaaagcgg
     1141 ttttcgcctg gcgtacccgc ctggcagtta aagacgatga taataagcca gccgataaac
     1201 gggattaaga acaacagtgc ccaccacgcc gagcggtcgg tgtcgtgcag gcggcggaac
     1261 tgaactgccc accacggtaa aaacaccaga ataccgtaga ttgtcgtcag gatgccttcg
     1321 ccaccagcgc gttgccagcc taacatttta tccagcagcc ccagcacgaa cgtaaagatg
     1381 atgttgacca gaataaacat ccagtactct ttgcgccgcg cacgaccccg gaaaccgaca
     1441 taatttttta gtactttcag ataccagtcc atttttgcct caattttgct caatcacttg
     1501 tttttaaagc agcaaattaa gaatagacgt gaatgaaatg gcagtaaata ttcgttcaag
     1561 gaattaataa agtgctgtga aggcgcgcta agactgatga caagcgcaaa atcgccagat
     1621 gcgctacgct tatctggcct gcgcgctcat tgcaacatat tgaatataaa tgttttgtag
     1681 gtttgataag gcgttcacgc cgcatccgac atgaacaaca tgcacttagc taattatcag
     1741 aggcacctcg taaggtgcct ttttttaacc gaagcgaaca tctcgtccat gcggttcatc
     1801 gagatcctgc catggaccaa ttgaaataat ccccgtaggg ttgatggtct tatggctgcg
     1861 gaagtaatga ttacggatgt gatcgaaatt gactgtttcg gcaattcccg gcatctggta
     1921 gatatcacgc aggaagccat acagattaag gtaatcgctg atgcggtgct tatcacactt
     1981 gaagtgggtc acatacactg gatcaaaacg gaccagcgtg gtccacaggc gaatatcggc
     2041 ttcggttagc tggttgccgg tcaggtaacg atgctggcct aaaatctgct ccagtcgcgc
     2101 cagcgattca aacactttcg ccaccgcctc gtcgtaggct tgctggctgg tggcaaaacc
     2161 agctttatac acgccgttgt taacggtgtc ataaatccag ccgttaagtt cgtcaatttt
     2221 cgtttgcagg gctggtgggt agtaatcacc cgcttttgcg cccagcgcat caaacgcggt
     2281 attgaacatg cggatgattt ctgctgattc gttgctgacg atggtgtggt tctttttgtc
     2341 ccacagcacg ggaacagtaa ctcgtccgct gtagtgtgga tcggcgtgga gataaagctg
     2401 atacagaaat tcatgttgat agagcgtatc tccggttgct cccggaaaac tgtcatcaaa
     2461 ggtccagccg ttttccagca tcagcgggtt cactacggaa acggaaataa acggttccag
     2521 tcctttgagt ttgcgcatga tcagcgtgcg gtgcgcccac gggcaggcga gtgaaacata
     2581 gagatgataa cgatctttct ctgcgataaa accgcctgtg ccagtggggc caggtgcgcc
     2641 atcggcagtg agccagttac gaaatgcgga agctgaacgt tgaaatttac cgccggtaga
     2701 tttggtatcg taccaggtgt catgccagac gccgtcaatc agttgcccca tttttctctc
     2761 ctccaatagc aaaaacgagg agcttcctcc tcgtttttta tcgttaagta tagcgcgtgc
     2821 ttaccacttt ttattcagca ggcggtcgat gctatacgcg cccggaccgg taattgccag
     2881 cagcaggaat ccgccagaaa ttgtcaggtt tttcatgaac atcagcgagt tgacgccttc
     2941 agcaaagttg ctgtgaaata aaaatgccgt cagcagcgta aagcccgcag taaacagggc
     3001 tgtggtgcga gtcaggaaac cgaacaggat tgccagacca ccaccgaact caagcagaat
     3061 caccagcggc agcataaaac ccgggacacc cattgcttcc atatattgtt gggtaccaga
     3121 gtaagcggtg atttttcccc agcctgcggt aataaacaga atcggcatta aaatgcgcgc
     3181 taccagtaca ccaacatctt ctaatttttt catcatactc tccactgcgc cactcaggct
     3241 gctgattgtt tttttgtgca agggcgcggt attagcgtcg ttgctgtcga tggagagaat
     3301 cataaacgtg gtgaatgatg attgttagca aggaaaactg tcaaaaatct tcaagaaatt
     3361 tgagggataa ggccggaatt gctccggcca gagggaagtt aaccgcgaag ctgttgctgc
     3421 ttgagggtcg ttttaaccag acgccaggcg ctccatacgc caaaaccgcg tctggcccag
     3481 cggaccagca tattaggatg gcgaatcgtc cagatcgcca tcacgctact gccaaccagc
     3541 gcccaggagc gcagacttag cagcatattc cagcgacgat cgtaagcgcc tgttgcctcc
     3601 agccattcac gacgactggc ggaaagatcc agccgttgtt gctggatctg gctaagcagt
     3661 tgcgccttac gtcgttcacg ttcgacttta ctgctcacgg gactcctcct cgagcagttg
     3721 ccgatcgttt gccagctcat ggcgtgtatg gcgcagcaag gtggatttac gcgatttacg
     3781 taacgtccag ataccgccaa ttagtgccag tagcagcaac accacggtgg tggcaatcat
     3841 cgcattcagg cgatattgcg gatcaaccgc ccaaataatt agcaccatca ggctcataag
     3901 accaaatgca gcgaaaagca tcgtcaggcc cagcatcagt aaaagttgaa agagattagc
     3961 tttttcctct tccagctcca ccaccgccag ccgcagacgt gtttccacca tttcaaccat
     4021 gatggaaaca attcgctgcc cgatgcccag aacgctttta ccgggccctt gcgcgtgatg
     4081 agtgtccgcc ataattaacg acgcgacagc agaacgccga gcactacacc gattgcagcg
     4141 ccaatgccca cgccggtcca cggattttca cgcacatact catcagcacg cgccgccgcg
     4201 acacgggttt gtttggcgat ggcatcaccg gtttcaccca ggcgataacg gctctgtttc
     4261 agtgcctgct ccgctttgct acgaatctta ctcaactctt ctttcgactt ctcgccagat
     4321 gagctaagca cctcttccag cgtatcggaa agggatttca actcagcacg cagatgttcc
     4381 gtagtgtgtt ctttcgacat agttttctcc aggtgagtaa gtagtgacta ttgtgagtta
     4441 gtagtcgcgc gcttccagct ttttcagctc ttcctgcgct tctgccagtt tccgttcgcg
     4501 tttggcaatc ttatcggcat cgcctttttg cttcgcctcg gctaaatctt gctggcgttc
     4561 cgccacctca tctttctgct ttgcgatttt cttgtgatga tcggcacgca gctggctatc
     4621 tgaacagttg gcccggactt cactcagggc tttattcaga ccgtcaatac gattctggtt
     4681 ttggtgtttt tcggcatagc tgatctcctt aaggatattt tgctcctttt cctgacacag
     4741 ggtagtggca taactaccgg cactaagagc aaagagagaa acagctaaag cgatgcggta
     4801 tttcattctt gaaccttcca ttgtgattcg gacctattac aaaggaacga ttgtccagac
     4861 aacgaagcgg tagtgttaac caccgcctca ttaatcatag acagttacgg tgaaatcacc
     4921 aaagtgagtg atatgtttcg gataacagga agttatccga agcgatgaga gttatcccgt
     4981 aaccgggtca gccactgcat agcctgactg ttattgtcct gctgcgcaat gatgtagcca
     5041 tggggcaatg ttctgtcgag gaccgctttt gcggcggcgc tctggtcgct ggagtcaaat
     5101 gtaattaaca gagtgtcgtt tttgggggtg atacttttga aaggaatgcc atgagcgtca
     5161 aggtgatgcc agatcgaaaa accgtctggc atcgttgtgc cttgatgaac ggcacgaatc
     5221 gccagcgtag actcttgctg gcgaaccgcg gaccaggcca gcaacagcgt gccgaccaga
     5281 agtaaaacag tgccggacca ggctagctgg cgaagcgaca tgcgaggtat ttgcatccct
     5341 tacccccgat ttccatattt ctttttccat aacacgacca gagaacctgc caggccaaac
     5401 accagcagca ccaccgggag cagcatcagg catgacatca gctggtcctc gtactttaaa
     5461 aataccggcg ttttgccgag catgtaaccc agagttgtca ggatcaatac ccacagcaga
     5521 ccgctcatcc agttgaaaaa ctgaaagcgc gcgttattca gccctgataa cccggcaatc
     5581 gtcggcagca gtgttctgac aaacgcaata aagcgaccaa ttaacagcgc cgataaaccg
     5641 tgtttatgaa aaagatggtg tgcgcgttga tgataatgcg cgggtaaatg agatagccag
     5701 ttttgtacgg tgcgggtatt gcccagccat cgcccctgaa tatagctgac ccagcagccg
     5761 aggctggcgg caacggtcag cagcagaatc gtttgcgggt aacccatcgc gcctttcgca
     5821 atcaacacgc cgaccaatac cagtaaactg tcgcccggta aaaaggccgc tggaagcaag
     5881 ccgttttcaa ggaacaaaat tacaaacaag acaaaataca acatgccaat catcgatgga
     5941 ttggccaggg tttcaaaatc ctgcgcccac agggcttgca gcaattgggt caaaagttcc
     6001 attcattatt cctggtgaat cggttaacgc cacgctggtt ttaaaagatg cactaccgca
     6061 atgttattgc tgttttggta tgttctgcgg gtttgataca tcaacacagc ctgaactatt
     6121 ccctgttaaa tcgtgagata agaaagcatt caaaaacata aattcaaatt gaatctaatt
     6181 gtaacaaagg tcaacgttgt tcgttatcaa aattacatgt ccgtggagtc tgattttgct
     6241 gaatgcaggg gagggtggcg agaggattta cacaggctga cactttatac ttttcgctta
     6301 ccgacgagac aaacaggcgc gtcgcagcgc ctgtcggaca ggtatttcat ttactgccac
     6361 tggtggccgt atcgagatgc accaccgggt tttcggcgaa tagatagcgg tcggcattga
     6421 actcgaagtc gtcgctggtt tcgttgaata acatgatctt ggtgttttcc aggtgctgcc
     6481 acattgccag cttagcagca tgaggatctt tgcgaatcag cgccttgagg atttgatcgt
     6541 ggtcatcaca ccagttatcg acggtacggg aatcaatgtg ttcgtgcagt tttttccagt
     6601 acgggttatg gctacgttgt gtccacattt tttcaacgat ggccgccagg gcggagttct
     6661 gcgtcgccag ggctacctga atgtggaact gtaaatccca ctcggaatca cggaagcatt
     6721 gttcgccgcg cgcctgttcc tgaatggcca tcagtttcat gatgtcctgt ttcgttacct
     6781 gagttgccgc gaattcggca atattacttt cgatgagctg gcgagcctga agcaactcaa
     6841 acggaccgta attggcgaat tccatattat tgtcagctgc ctgctgatgg cgcggctggt
     6901 tggaaaccac atgaatacct gaacctttac gcacttcgac atagccttca acttccagca
     6961 tgatgatggc ttcacgaact accgtacggc tgacgttctt ttcatcggca ataaagcgtt
     7021 ctgcaggcag tttatcacct accagataga cgccctgttc gatgcgctct ttcaggtcag
     7081 cggcaagttg ttgatacaaa cggcgtggtt cagtgatttc catatgcgtc tcccggagtg
     7141 gaatgcggac gaagttatca taccacttta ttcatgggta tgccattggt catatataaa
     7201 tttggtgatt gaattaatgt attgaattaa tattgtgaat atttataaat tcctaaaaaa
     7261 taagtatcgt cattgtgaaa gctactaaat ttcagtaagt taattcccag cctttaaatt
     7321 taatattaac taaaaatgct tttttatatt gacttacaat aaatctacat ctctaataca
     7381 ccaacttttt ggaaacaata ttgtaagatc aaatttatat ggtggcgaaa attaatctct
     7441 tatcacggct tacgccgctt ttattcgtgt tcgctccttt tttggtacag tcaaatatga
     7501 ctgtttatcc tatggctgtt tccattaata gtcagggtga aggcaacgtt cgcgtaatat
     7561 ctaaaagtaa tgaagttcag tacatcaagg cgacagtatt ccgtatcgat aatccatcga
     7621 cgcctcagga aaatgaagtt gagattaaat ccggtgatgc aaatcatctg gttgttatgc
     7681 cacctaaatt cgctttacca gccggtagta gcaaaaccgt acgttttgtt gcgatggaac
     7741 cagagcaaaa agagaaaaat tatcgcgtta aatttgaagc ggttcccagt attgatgacg
     7801 ttgccacaga taaaaaagat ctctctatgc agttaacagt taacttaatt tgggggattg
     7861 ttgttagtgt tccacctcag caacctattg ctaagttaga agtaaatgct gcccaaaaat
     7921 tagttaatgc agggaatcaa cgcttaaaga ttttaacgat cgcttattgt aaaaataata
     7981 gcaaagaaaa ttgtaagata cagaccgtaa ataaaaacat cttccctggt caggaaagaa
     8041 atcttgaaag catatctggc tacgacaaaa tcgttgtcaa atataataac tggatcacca
     8101 aagataatgg cgagtttgaa ctggcggtcc attaattatc attgtaataa acgaaggaat
     8161 agtaatgaaa aaggtatttg caaaatctct tctggtcgca gcgatgtttt ctgttgcagg
     8221 ctccgcattg gcagtacaaa aggatattac tgtaacggcc aacgttgatg ccgctctgga
     8281 tatgacacag accgataaca ccgcgttgcc gaaagcagta gaaatgcaat atctgccggg
     8341 gcagggtctg caatcttacc agttgatgac caaaatctgg tctaacgacg ttaccaaaga
     8401 tgtaaaaatg cagttagttt ccccggcgca actggttcaa agcctggatg ctagcaagat
     8461 tgttccgctg acagtaacct ggggcggtga agaaattaaa gctgatgcgg caaccacttt
     8521 taccgcaacc aaaatctttg cttccgatgc gttaactaac ggttctctgg ctaaaaacct
     8581 gatgtttgct caaaccacca aaggtgttct ggagacaggc atctaccgtg gtgtagtaag
     8641 catttatctg tctcaggata tctaatccag ataacagaat taacacgtaa caaagggagg
     8701 ggtagccctc ccttatttta actgttttag tatttgttat ggatttcagg attgccatgg
     8761 ataaaaaatt actggctctt ttgatcctgg cgagtctcag cccggcagag gcgacattaa
     8821 ccaaaattcc cgcagggttt gaggttattg ctcagggaca gcaggagtat atcgaggttt
     8881 atttttcagg gaaaagtctc ggtaaatatt atgcaatggt taatcttgat accgtaacat
     8941 ttcttgatcc agcaagtcta tataacaagc tggaactgga tgtagacgat cagaaaatcg
     9001 cgcatatagt gaaagaaaaa ttatcgcagc cgctagctcg ccacggtgaa ttggcttgcg
     9061 gttatgtacg tactgactca gggtgtggtt ttctgaatac cgatacgctg gaaataatct
     9121 ataatgatga agaaagttcg gcaacgttgt ttattaatcc gcaatggaat tcagctttcg
     9181 atgcgaagtc attatattta aatccagaca aaaatactgt taatgctttt atacatcagc
     9241 aagacatcaa tgttctggca caggatgatt accaatcgtt gtctattcag ggaaacggtg
     9301 cgctgggaat aacagaaaat agttatattg gtgcacactg gaatttcaac ggttatgatg
     9361 cagatgatgt cagtgacagt aatgctgacg tcagcgatct ctattatcgt tatgattttt
     9421 tacgtcgtta ttatgtgcag gcgggtcgca tggacaaccg cacactattt aatgcacaag
     9481 gcgggaactt tacctttaac tttctgccac tcggtgcaat tgatgggatg cgtatcgggt
     9541 cgacactgag ctatttaaac caggcgcaaa gccagcaagg aaccccagta atggttctgc
     9601 tttcgcgcaa ttctcgtgtt gacgcttatc gtaatgagca acttttggga tcgttttatc
     9661 tcaatagtgg ttcgcaattt attgatacca gttcctttcc gcctggcagc tacagcgtgg
     9721 cattaaaagt ctacgaaaat aaccaactca cccgcaccga gcttgtgccg tttaccaaaa
     9781 ccggcggtct gactgacgga aatgcgcaat ggttcttaca ggcaggtaaa actacatcac
     9841 aggtttctga tgatgaaagt tcagcttatc agctaggagt acgcctgcca ttacatccgc
     9901 aatatgagct ctacgcaggg ctggcgaatg ccgatgatgt gagtgctttc gagttaggta
     9961 ataactggac ggcagattta ggcggggtgg ggaatcttgc aatcagcgcc agcgtgttcc
    10021 gtaacgatga cggcggcaaa ggtgatatgc aacaggccaa ctggagtaat ccgggatggc
    10081 cgacgttggg cttttatcgg accaactctg acggcgatgc ttgtacaacc gacagcagag
    10141 agagctataa cgccttaagc tgttatgaaa gtatttccgc gacggtttca cagaattttg
    10201 tcggctggaa tatgatgctg ggttataccc gcacacaaaa taacactgat gatagtttgc
    10261 gttgggataa acagcagagc tttgaaaata actatcttcg ccagacaaca gcgcaaagta
    10321 tctctgaaac tgtacaactt agcgcttccc gcgcttttgt gatgcgtgac tggattttga
    10381 gtacgtcagt cggtgttttc catcgtaatg acaacggtgg cgataacgac gacaacggct
    10441 tgtacttatc gttttcgtta tctgacacgc caacgatgga tagcaataac aacagctatt
    10501 caacaaatgt gtctacggat tatcgttata gcgaacagga tggcgatcaa acgtcatggc
    10561 agttatcgca taccttttat aacgattcat tcagccataa agaacttggc gtaaccgtcg
    10621 gaggcctgaa caccgatacc ataaacagcg cggttaacgg gcgttgggat gggcaatacg
    10681 gaaatgtcta cgctaccgta tctgatagtt atgaccgtaa gaatcatgat catctttcgg
    10741 cctttacggg cacttatagc tctacgctgg ctgtcagtcg ctatggcgtt aatttgggag
    10801 ccagtggtac agacgatttg ctgggtgcgg tactggtgga tgtgaaagtc ttctctgaac
    10861 aggatgaaga tagtcaggat ctgcaactcg aagcgcgggt ggcaggcagc cgaactttgc
    10921 agcttggtca aagtgacagt gtgttgttcc cttatcctgg atttcagtct ggttttgttg
    10981 aggttaacga cagtagccag ggcaatcagc aggggacaac aaacatcatt aacggtgcag
    11041 ggaatcgtga attaatgttg ttgcctggca agctgcgcta tcgcgaagtg tctgccagct
    11101 ttaactacaa ctatatcggt cgtttattac tgccggcagc ggtgaaaaaa ttcccgattg
    11161 tggggctgaa cagcgccatg ttactggtag ctgaagatgg cggatttaca cttgaaatta
    11221 acggcagcga aaaagagctc tatctgcttt ccgggcagca attccttaag tgtccactga
    11281 gtgttgtaaa gaaacgcgcc agcattcgtt acagcggaga tgttacttgt agtgtggtga
    11341 cttattcaca attaccggag tcgattcagg ttcaggcaca gttgaaacag cctaaattac
    11401 gtggaaacgt tcagacggcg caaagggagg ttgcaccatg agaaatcgat tgattgcggc
    11461 gatattgggc ttgtttggca cgctcactgg cgttcaggca gctcctgacg tgaccagtga
    11521 aattacgtat gatttggcat ctggtagagc ggattattac ttctggaagg atgaagcgtc
    11581 ggcaggaaat aatggatata tgtggtatga atgttcgtat cctgacctcc aacaaacctg
    11641 tacagctaat ggaaatatat cgacagtaca aatctattta actgaacaac gcagtgggat
    11701 gcgttggccg gtaaaactca aaggatttaa aacagccatt gtaagtagcg atgaagcgcc
    11761 gccaggatgc aaggggggca aagggcttca gacgaatctt aaggattcta atagatcttc
    11821 atgtacagaa gatggtcaac attattatat atacgataca aagtttctta cgctctacct
    11881 tgagcagaca gagatgaaga atttgccgat tggtggcgtc tggaagggga aagttaaatt
    11941 acattcgaac agcccggccc aggactattt cgcaaatatt accctgaata cgctcgaccc
    12001 caaccatatt gacgtgttct tcccggagtt cgcccacgcc acgccaaggg tgcagttaga
    12061 cttgcatcca acaggaagcg ttaacggcag caactacgcg caagatctga ccatgttgga
    12121 catgtgcctg tacgatggtt ttaacggtaa tgccatcagt tatgaaatca tgctcaaaga
    12181 tgaagggcga ccagctgcag ggcgcagaga cggttacttc tctatctatc gtcagggagg
    12241 gaccaccacc gacgagggag aacgcattga ttaccgggtc aaaatgtaca acccggaaac
    12301 cggtgggcaa attgatgtgc gcaataatga aaatatggtc tggaacagca ttaacctgaa
    12361 acgtgtgcgt ccggtggtac tgcccggtat tcgctatgcc gtgatgtgtg tgccgacacc
    12421 cttgactctg gcagtcgata aattcagcgt gatggataaa caggccgggt actacatggg
    12481 caaattgtca gtaatcttta cgccttcctt gccaaccatc aattaaccct gcaaaccgga
    12541 caaacgcttt tttctatata aacgacgaag ccgtctgata aggcggcttc gtcattacaa
    12601 cttaagttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
    12661 tcgccggttt gttctgcaac acggtccaga taaccagcgc acctaacagg tcaaatactg
    12721 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
    12781 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
    12841 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
    12901 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
    12961 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
    13021 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
    13081 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
    13141 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca
    13201 ggaaacgcgg cagcgcgata ccccagaact gacggttacg caggatctga ccaacggaca
    13261 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
    13321 cttcatcggt cagatgcttc tggtcgcgtg gatgtttata gaaaatcagc catgccatcg
    13381 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcattacga
    13441 ttgcccatac caccagcggc ggcgcaatca tcgcaccaat cgaagaacct acgttaaagt
    13501 aacctaccgc gatggaacgc tctttcgccg ggaaccattc ggagctggct ttcagacccg
    13561 ccgggatcat cgctgcttcc gcggcaccga ctgcaccacg agcaatcgcc aggccacccc
    13621 agctaccagc cagcgcggtt gcaccacaga acacggccca cagcacagca aacattgcat
    13681 aaccgatttt cgtacccagc acatccagta catagcccgc taccggttgc atgaccgtat
    13741 aagcagcaga ataggctgcg atgatatagg aatactgttg ggtggagatg tttaactctt
    13801 ccatcagagt tggcgcagct gccgccacag tgttacgcgt caggtaacca agcacggtgc
    13861 caagcgtcac cagtgcgatc atataccaac gtaacccttt aattttacgc atgtaaaacc
    13921 tcatcgtttt gttatatccg caggaagcgc ggccatcatc ccgccagagg ctgaagatgt
    13981 tagttacgac cgggtaaccg aaaaaaaacc ggcacgcccc gaacgttgcc atgaaagttt
    14041 cgtgctaatt cggcttccgt accggttaac ccgattcatc taacaaatga atgaatcggc
    14101 aatttgcatt ccgtcgactt atgatttgcg acggcagaaa gataacttgt catacaactt
    14161 taaaaggtga gagccatcac aaatgtgcga atatttgtag ggacattacc tgacgacagc
    14221 gaggccagta ctggcgcggc ctgcagagag atttaccact ttgagagtaa tttttttaac
    14281 tacgtttatt gatctaactc acgaaaatat cttcggactc tggaaattgg tgtgataact
    14341 ttgtcagcat cgcaccataa gcaagctagc tcactcgttg agaggaagac gaaaatgact
    14401 ccgtttatga ctgaagattt cctgttagat accgaatttg cccgccgtct gtatcacgac
    14461 tacgcaaaag accagccgat tttcgattac cattgccatt tgccgccgca gcagattgcg
    14521 gaagactatc gttttaaaaa cctgtatgac atctggctga aaggcgatca ctacaaatgg
    14581 cgcgctatgc gtaccaacgg tgtggccgag cgtctgtgta ccggtgatgc gtctgaccgt
    14641 gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta tcggcaaccc gttataccac
    14701 tggacgcacc tcgaactgcg tcgtccgttt ggtatcactg gcaaattgct ttctccgtca
    14761 actgccgatg aaatctggaa cgaatgtaac gaactgctgg cgcaggataa cttctccgcg
    14821 cgcggcatca tgcagcagat gaacgtgaaa atggtcggca ccaccgatga cccgatcgat
    14881 tctctggagc atcacgcaga gatcgccaaa gacggctctt tcaccatcaa agtgctgccg
    14941 agctggcgtc cggacaaagc cttcaacatc gaacaggcga cctttaacga ctacatggcg
    15001 aagctgggcg aagtttccga taccgacatt cgccgctttg ctgacctgca aactgccttg
    15061 accaaacgtc tggatcactt cgccgctcac ggctgtaaag tgtctgacca tgcgctggac
    15121 gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca gcattctggc gcgccgtctg
    15181 gctggcgaac ccctgagcga gcacgaagtg gcacagttca aaactgcggt actggtgttc
    15241 cttggtgccg aatatgcacg tcgcggctgg gtacagcagt accacattgg cgcgctgcgt
    15301 aataacaacc tgcgtcagtt caaactgctg gggccggatg taggctttga ctccatcaac
    15361 gaccgtccga tggcggaaga gttgtctaag ctgctgagca agcagaacga agaaaacctg
    15421 ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata acgaagtgct gggcaccatg
    15481 atcggtaact tccagggcga aggtatgccg ggcaaaatgc agttcggttc cggctggtgg
    15541 tttaacgatc agaaagacgg tatggaacgt cagatgaccc aactggcgca gcttggtctg
    15601 ctgagccgct ttgtcggtat gctgactgac agccgtagct tcctgtcata cacccgtcac
    15661 gaatacttcc gccgcattct gtgccagatg atcggtcgct gggtggaagc gggcgaagca
    15721 ccggcggaca tcaacctgct gggcgagatg gtgaaaaata tttgctttaa caatgcgcgt
    15781 gactacttcg ccattgaact gaactaaggt ctggggttga tatgcaatac atcaagatcc
    15841 atgcgctgga taacgtcgcg gtcgctttag cagatttggc tgaaggcaca gaagtcagtg
    15901 tcgataacca gactgttacg ctgcgccagg atgttgctcg tggacataaa tttgcgttaa
    15961 cggatatcgc aaaaggggcc aacgtcatca aatatggcct gccgattggt tatgcattgg
    16021 cggatattgc ggcgggggaa cacgtccacg cccacaatac gcgcacgaat ctgagcgatc
    16081 tggatcagta tcgctatcaa cctgattttc aggatctgcc tgcgcaagcg gcagatcgtg
    16141 aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt gcgtaatgag ctgtggatcc
    16201 tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat ccagaaccgt ttcctgaaag
    16261 agaccaacaa cgccgaaggt accgacggcg tgttcctctt cagccacacc tacggctgct
    16321 cacaactggg cgacgatcac atcaacaccc gcaccatgct gcaaaacatg gtgcgccacc
    16381 cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga aaacaaccag gttgccgcat
    16441 tccgtgaaac gttgggcgat atcgatcctg aacgcgttca tttcatgatc tgccaacagc
    16501 aggatgatga gatcgaagcc gggatcgagc atttgcatca gctgtataac gtgatgcgca
    16561 acgataaacg cgagccaggc aaactcagcg aactgaagtt tggtctggag tgcggtggtt
    16621 ctgacggtct ttctggtatt actgctaacc cgatgctggg gcgtttctct gactacgtga
    16681 ttgctaacgg cggtactacc gtactgaccg aagtgccgga gatgtttggc gcagagcagt
    16741 tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa actggtcacc atggtcaacg
    16801 acttcaaaca gtactttatt gcccatgacc agccgatcta tgaaaaccca tcgccgggga
    16861 acaaagcggg cggtatcacc acgctggaag ataaatccct tggctgtacc cagaaagcgg
    16921 gttccagcgt cgtggttgac gtgctgcgtt acggcgagcg tctgaaaacg ccggggctga
    16981 acttgttaag tgcgccgggt aacgatgccg tagcgaccag cgccctggcg ggtgcgggct
    17041 gccatatggt gctgttcagt actggtcgtg gcacgccata cggcggcttt gtaccgacgg
    17101 tgaaaatcgc taccaacagt gaactggcgg cgaagaaaaa acactggatc gactttgacg
    17161 cgggtcagct gatccacggt aaagcgatgc cgcagttgct ggaagaattt atcgacacca
    17221 tcgttgagtt tgccaacggt aaacaaacct gcaacgagcg taacgacttc cgcgaactgg
    17281 cgatcttcaa aagcggcgta acgctataaa gtcgaaatga gttgttaata aaaaacggcg
    17341 tttcataatg tgaagcgccg tttgcttttc catacctgat aaggatttat catgaccgcg
    17401 tattggctgg cccagggcgt gggtgtcatc gcctttctga ttggtatcac aacatttttc
    17461 aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct atagcgccgt tattggcgta
    17521 catttttttc ttctgggcac ctatcccgct ggtgccagtg ccatccttaa tgccattcgt
    17581 acattgatta ccttacgcac gcgcagctta tgggtaatgg cgatttttat tgtgctgact
    17641 ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac tattgccggt tatcggtacg
    17701 attgtcagta cctgggcgct gttccgctgt aaagggctca ccatgcgctg cgtgatgtgg
    17761 ttttcaacgt gttgctgggt gattcacaac ttctgggcgg ggtcgatagg cggcacaatg
    17821 attgagggta gttttctgct tatgaatggc ttgaatatca ttcgtttctg gcggatgcag
    17881 aaaaggggaa ttgatccgtt taaagtagag aaaacccctc ccgccgtaga cgaaaggggt
    17941 taaacaatta attacgcagg gcgctatttg ccagacgatc gtcttccgcc tggcaagctg
    18001 ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt ttcgcaagag tcctgcaata
    18061 cgccgatgat aaagccgacg gcaaccacct gcatggcgat atcgttcgaa ataccgaaca
    18121 tattacaggc cagcgggatc agcagcagag aaccccccgc cacgccggat gcgccacagg
    18181 cacacagaga agccaccacg ctcaacagca gcgccgtggg cagatcgacc gggataccca
    18241 gcgtattaac cgcagccagc gtcaacacgg taatggtgat tgctgcgccc gccatattga
    18301 tggtggcacc cagcggaata gaaacggaat aggtatcgcg atccagattc agcttttcac
    18361 acagtgccat attcaccgga atgttcgccg cagagctgcg ggtgaagaag gcatacacgc
    18421 cgctttcgcg caggcacagc agcaccagcg ggaacgggtt acgacgaatt ttccaccaca
    18481 ccagcaatgg gttaaccacc agcgccacca gtaacataca gccaaccagc acgaccagca
    18541 gttgcgcgta gccccacagt gtggagaaac cggtggttgc cagggtagaa gaaaccagac
    18601 caaaaatacc aatcggtgcg aagcgaatga ccagtttcac cataaaggtg acggcattcg
    18661 acatatcgtt gaccaggttt ttggtggtct cgttaccgtg acgcagtgcg aaaccgaggc
    18721 caattgccca caccaggatc ccgatgtagt tacctttcag cagcgcgtcg atggggttgg
    18781 aaaccatgct cattaccagc ccgcgcatca cttcgacaat gcctgacggc ggcgaaatat
    18841 cacccgcgct actggataaa tgcagggtag aagggaaggc aaagctgaag actactgcgg
    18901 ccagagcagc agagaaggta cccagcagat agaggaacaa aatagggcgg atattggttt
    18961 tctgcccgtg ctggtggtta gcaatagatg ccatcaccag catcaacacc aggatggggg
    19021 caacggcttt cagtgcgccg acgaacaaag tacctaacag accaacagct tccgccgcgg
    19081 gttttgagat ccatgccaga agaatcccca gaacaaggcc gaccaggatt tgttttacca
    19141 ggctgccatg agccagacgc cggaatagcc ccggtgaacg ttgcgtagtc atttttcgat
    19201 cctttcattg tgttgtcgca catccctggt gcgttctgtc gacgcatctt tcaggatgta
    19261 acaaagtgtt tgcaaggtcg agtataagga aagttacagc ttcgggaagc gaaaaatgct
    19321 ggattttaaa gagaggtcat attttgcaac ttaatggtta catttatttg acataaaaat
    19381 aaagccaacg tgcggcttcc agactgatga caacatcaaa aaaggactcg gtcagtcccc
    19441 tcgccccgtt tggggagagg gttagggtga ggggaacagg ccagcatcgg tgcgaaaaat
    19501 taccctcacc ccgaccctct ccctggaagg gagagggaga aaacggtgct catgttaatg
    19561 acagggtaca cggcttaggc agattaaaaa ttatccaccc cgctgcttat catgccgata
    19621 attcacccag gcgttgataa taaacgtcat caccagaatg ccaaacacca cgcccagcga
    19681 gacggcgatt ggaatatggt agaagtcgac aatcagcatc ttgataccga taaacaccag
    19741 aatcaccgcc aggccatatt tgagcatcga gaaacgctct gctacgcccg ccagcaggaa
    19801 atacatcgca cgcaggccga ggattgcaaa caggtttgag gtcagcacaa taaacggatc
    19861 agtggtcacg gcgaagatag ccggaatgct atccacggca aaaatcacgt cgctcaattc
    19921 caccagaatc agtaccagca tcagcggtgt ggcgtacagc aatccattct tacgcacaaa
    19981 gaaatgctcg ttgtcgatgg tgtcggtcat gcgcaaatga ccgcgtagcc agcgcaccag
    20041 cggcttgtcg ccaatgcctg attcatcttc atgggcgagc gccatcttca cgccggtaaa
    20101 cagcaggaag gcaccgaaga tatacaggat ccagtcgaac tgcgaaatta gccagctgcc
    20161 agtgaagatc atgatggtac gcagaacaat cgccccgagc acgccataca ccagcacgcg
    20221 gcgttgtaat gccgccggaa cagagaaata gctgaacaac atcagccaga caaagacgtt
    20281 atcgaccgcc agcgatttct caatcagata gcccgtgaga aaggccagtg cctgtggatc
    20341 ggcaacggcg cgaccttcag tttgcaccag ataccaccag aaagcggcgt taaacagtaa
    20401 cgacagcgtc acccagacca gcgaccaggc cgcagcctgt ttcatggtca tggcatgtgc
    20461 cccacgacgc ccctgcaaca acaggtcgat agccagcata atggcgacaa caacagcgaa
    20521 tccgccccat agcaacggcg tgccgacagt attcatagaa gttccttaca cataaaaaaa
    20581 cggccaacgt cggaagacgt cagccgctgc tttttatgca tagacctcgc cttccggcaa
    20641 ggtctcactt acaacaaaaa aggaatacgt ctgcgtattc ctttcgggtt gcccggtgac
    20701 cggatgtggt ttttcacaca tcgtaatgac gatcgaccgg caatgaagtt actccccttt
    20761 gcaggtaaca aagtatgaca gaccattcag ccagtcaatg gtctgttaca taccttttac
    20821 actgttttac tttggcaatt atagttttac gctatctgcc ggaaaaatca ccccagtctg
    20881 gcggcggatc tcggtcagca gtttggcggt gatgcgactg accgccagcc ccggatgatc
    20941 caccagatgc tcatccacca gggtagcgaa cagctctgct tcgtagagca tggtattaat
    21001 atgctgcggc tgggtgagat cctgcatttg gctgccacgc ggcacgaagc acactttctg
    21061 gcattcagac agtttttcaa tcaccagcga tcctgcttcg ccctgaatct cgctcgccag
    21121 gacagaatca ctgacttttg agtgctgcaa ggtgacgctg aaatcaccgt aatccatcac
    21181 caccacgccg tgggcatcta caccgcttgc cagcaaactg gcggttgcct gcacgctttt
    21241 cggctcacca aataacgcca ccgccgacgc caggcagtaa aagccgatat ccataattga
    21301 accgttagag aatgacggat taaaggtgtt gggattttca ccgtccaggt aacgttgata
    21361 gcgcgaggaa tactggcaat agttgaaaaa gactttacgc aatttgccga ctttcggcag
    21421 cgcctggcgc agcaaatgaa agttcggcag gcaggcggtt ttaaatgcct caaacagcac
    21481 cacctgattt tcccgcgcac aggcaatggc ggcatccact tccgccagat tcgacgccag
    21541 tggtttctcg caaatcacat gaattttatg gctaaggaaa agttgtgtct gggaaaagtg
    21601 cagggcattc gggctggcaa tatacaccgc gtcaatggca tcgctttccg ccatcgcttc
    21661 cagcgaggta aacagatgct cgacagaaaa atcattggcg aagtgctgag cctgctcaag
    21721 gctgcgggaa tatacggcgg ttaacttgta tttaccgctc tcatgggcgg cctcgacgaa
    21781 ctggcgagtg atccagttcg taccaatcac agcgaaacgt atcataacgc gtggatactc
    21841 cataaaggga ttctgtcgtc aatgtagcac gcctttttca tcttcgacgt gcgcagatac
    21901 gcattattct tttgccggac agccatgctc actaaacggc ccaatcatga ccgatggccc
    21961 gagatatttc ggctggcgat ggaggatttt ggcgtcaatc acggcgctga cacgagcgag
    22021 tttctcccgc agttgagtat aaaaaccgtg cttcatattg aggtcaggcg cgttaaaacc
    22081 gatagcgtcg atgccgtact gttttgccag ccagatggcg cgttgattat gaaattcttg
    22141 cgagataatg gtgatatggt tttcaccaaa aacctttttg gcacgcacta ccgaatccag
    22201 cgttgagaat ccggcatagt cgcagaagat cactttcgcc ggtacgcctt tagcgatcaa
    22261 agcctgctgc atacctgagg cttcatcgta atttttacgt ccgttatcgc cacttaccag
    22321 tagccatttc acttttccgg cgtggtacaa cgctgctgcg gtatcaatac gtcgggtaaa
    22381 gtagcgatta cccggcctgg cccccagcaa taagcccacg ttacgcgccg gaactgcgtt
    22441 gacatcgctc caggtcagct gcttgctggc gttgaccata acgcgatcgg caataaaaat
    22501 ggtcgctcct gctataagca acaacaaaca gccaatctta agagtccggc gagaaaaaca
    22561 aatacggaga agaaggcggg gctgcatggt cgttatcctt aacgaaaggg gggaggtcga
    22621 ttttaggcgc taaagaaaga aaagataacg cctgacatca ttttcagatt ttgtcctgcc
    22681 caagcgacaa ctgcgttagc cacagccggg tgtcgaactc aagctggtgg tactgcggct
    22741 ccatatggca gcacaactgg taaaacgctt tgttatgctc tttctccttc aggtgcgcca
    22801 gttcgtgcac cacgatcata cgtaaaaacg gttccggcgc gttgcgaaac acagtagcga
    22861 cgcggatctc tgcttttgct ttcagcttgc cgccctgcac acgagaaaca gcggtatgta
    22921 acccgagcgc gttttttagc acatggatct tattgtcata catcacttta ttgatcggcg
    22981 gggcattgcg cagaaactga ttttttagat cctgagtata ttgccagagg gctttatcgg
    23041 tggcgtagtc gtgtgttccc ggatagcgtt ttgccagcac atcgcccaga cgctgttcgt
    23101 taatcagcgt tcgcacctgg gaaagtagct gctcgggata accctgaaga taagtaagat
    23161 tgctcataac cgcccacgaa aaagagaaaa agggtatact cacgcaccct tttcaggggc
    23221 aacgtcgaaa ttttatcatt caggagggcc gatgagccac ttagacaacg gtttccgttc
    23281 actgacacta caacgttttc cggcgacgga tgacgttaac ccgctacagg cgtgggaagc
    23341 ggcggatgaa tatttgctgc aacagttgga cgacacagaa atccgcggcc cggtgttgat
    23401 cctgaatgat gcctttggtg cgttaagctg cgcgctggcg gaacataagc cgtacagcat
    23461 tggcgactca tacatcagtg aactggcgac gcgcgagaat ttacgcctca acgggattga
    23521 tgagtcgagc gtgaagtttc tcgacagcac cgccgactac ccgcaacagc cgggtgtggt
    23581 gctgatcaaa gtgccgaaaa cactggcatt gctggaacag caactgcgtg cgctgcgcaa
    23641 agtggtcacg tcggatacac gtattattgc cggtgccaaa gcccgtgaca ttcacacttc
    23701 cacgctggag ctgttcgaga aagtgctcgg cccgaccacc actacgctgg catggaaaaa
    23761 agcgcgcctg attaattgca ctttcaatga accgccgctg gccgatgcgc cgcagaccgt
    23821 tagctggaag ctggaaggta ctgactggac tatccacaac catgcgaatg tcttctcccg
    23881 caccgggctg gatattggcg cacgcttctt tatgcaacat ctgccagaga atctcgaagg
    23941 tgagattgtc gatctcggct gtggtaacgg cgttattggc ctgacgctgc ttgataaaaa
    24001 cccgcaggcg aaagtggtgt ttgtcgatga atcgccgatg gcggtcgctt ccagccgaat
    24061 gaatgttgaa accaacatgc cagaggcgtt ggatcgctgc gagtttatga tcaacaacgc
    24121 gctctccgga gtggagcctt tccgctttaa tgctgtgctc tgcaacccgc cgtttcatca
    24181 gcaacatgcg ctgaccgata acgtcgcctg ggagatgttc caccacgccc gccgctgcct
    24241 gaaaatcaac ggcgaactgt atatcgttgc caaccgtcac ctggattact tccataaact
    24301 gaagaagatt ttcggcaact gcaccaccat cgccacgaat aataaatttg tggtgctgaa
    24361 aacagtgaag ctggggcgtc gtcggtaaga ttgggagtta cctgccggat gcggcgttaa
    24421 cgccttatcc ggcctacgtt cagcacgtta ctcccaggcc ggataagacg cggcaagcgt
    24481 cgcatccggc aatgtctgcc cagttctgga atcgcgcttc cataaaacaa aaaatccctg
    24541 tcaccatcct ctacattctc ttgtttagcg ttgttcaacg tttatgctac atacgtgaag
    24601 ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag gggaaaaaat
    24661 gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgcggtgg caccttttct
    24721 ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag atcatctgct
    24781 gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gccaaaataa ccgcgtggga
    24841 agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc ctggcggtat
    24901 agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc tgccggaaat
    24961 tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa cgctggaaca
    25021 cgctaaaaaa ttggcaacgc gattcggcat ttc
//
LOCUS       DAZGXZ010000069        24996 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000069 DAZGXZ010000000
VERSION     DAZGXZ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24996
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.069"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(105..188)
                     /gene="yohP"
                     /locus_tag="ACHKJF_002944"
     CDS             complement(105..188)
                     /gene="yohP"
                     /locus_tag="ACHKJF_002944"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP0950267.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            412..1848
                     /gene="mdtQ"
                     /locus_tag="ACHKJF_002945"
     CDS             412..1848
                     /gene="mdtQ"
                     /locus_tag="ACHKJF_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP0950268.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAL
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1901..2662
                     /gene="yohF"
                     /locus_tag="ACHKJF_002946"
     CDS             1901..2662
                     /gene="yohF"
                     /locus_tag="ACHKJF_002946"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0950269.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2792..3370)
                     /gene="yohD"
                     /locus_tag="ACHKJF_002947"
     CDS             complement(2792..3370)
                     /gene="yohD"
                     /locus_tag="ACHKJF_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0950270.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            3540..4127
                     /gene="yohC"
                     /locus_tag="ACHKJF_002948"
     CDS             3540..4127
                     /gene="yohC"
                     /locus_tag="ACHKJF_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP0950271.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4301..5233
                     /gene="pbpG"
                     /locus_tag="ACHKJF_002949"
     CDS             4301..5233
                     /gene="pbpG"
                     /locus_tag="ACHKJF_002949"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP0950272.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5271..6986)
                     /gene="dld"
                     /locus_tag="ACHKJF_002950"
     CDS             complement(5271..6986)
                     /gene="dld"
                     /locus_tag="ACHKJF_002950"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0950273.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            7182..9479
                     /gene="bglX"
                     /locus_tag="ACHKJF_002951"
     CDS             7182..9479
                     /gene="bglX"
                     /locus_tag="ACHKJF_002951"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP0950274.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9690..10607
                     /gene="osmF"
                     /locus_tag="ACHKJF_002952"
     CDS             9690..10607
                     /gene="osmF"
                     /locus_tag="ACHKJF_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP0950275.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10614..11771
                     /gene="yehY"
                     /locus_tag="ACHKJF_002953"
     CDS             10614..11771
                     /gene="yehY"
                     /locus_tag="ACHKJF_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP0950276.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            11764..12690
                     /gene="yehX"
                     /locus_tag="ACHKJF_002954"
     CDS             11764..12690
                     /gene="yehX"
                     /locus_tag="ACHKJF_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP0950277.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSRVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            12695..13426
                     /gene="yehW"
                     /locus_tag="ACHKJF_002955"
     CDS             12695..13426
                     /gene="yehW"
                     /locus_tag="ACHKJF_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP0950278.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13407..13514)
                     /gene="yohO"
                     /locus_tag="ACHKJF_002956"
     CDS             complement(13407..13514)
                     /gene="yohO"
                     /locus_tag="ACHKJF_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP0950279.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(13574..14305)
                     /gene="mlrA"
                     /locus_tag="ACHKJF_002957"
     CDS             complement(13574..14305)
                     /gene="mlrA"
                     /locus_tag="ACHKJF_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP0950280.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14527..16212
                     /gene="btsS"
                     /locus_tag="ACHKJF_002958"
     CDS             14527..16212
                     /gene="btsS"
                     /locus_tag="ACHKJF_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP0950281.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16209..16928
                     /gene="btsR"
                     /locus_tag="ACHKJF_002959"
                     /gene_synonym="yehT"
     CDS             16209..16928
                     /gene="btsR"
                     /locus_tag="ACHKJF_002959"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP0950282.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16975..17445
                     /gene="yehS"
                     /locus_tag="ACHKJF_002960"
     CDS             16975..17445
                     /gene="yehS"
                     /locus_tag="ACHKJF_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP0950283.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17486..17947)
                     /gene="yehR"
                     /locus_tag="ACHKJF_002961"
     CDS             complement(17486..17947)
                     /gene="yehR"
                     /locus_tag="ACHKJF_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP0950284.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18072..20072)
                     /gene="yehQ"
                     /locus_tag="ACHKJF_002962"
     CDS             complement(18072..20072)
                     /gene="yehQ"
                     /locus_tag="ACHKJF_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP0950285.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCTTAQPTEKEE
                     AWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHVTSSDDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(20069..21205)
                     /gene="yehP"
                     /locus_tag="ACHKJF_002963"
     CDS             complement(20069..21205)
                     /gene="yehP"
                     /locus_tag="ACHKJF_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0950286.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21198..23477)
                     /gene="yehM"
                     /locus_tag="ACHKJF_002964"
     CDS             complement(21198..23477)
                     /gene="yehM"
                     /locus_tag="ACHKJF_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFP0950287.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAVLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALQGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(23488..24576)
                     /gene="yehL"
                     /locus_tag="ACHKJF_002965"
     CDS             complement(23488..24576)
                     /gene="yehL"
                     /locus_tag="ACHKJF_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP0950288.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5857 a         6395 c         6793 g         5951 t
ORIGIN      
        1 aacgcttcag cgattttgac gccaccaaca ccggagctgt ttgcaaatca agtacacaaa
       61 atatctagtt cctgacctga caacttatat gccatatctg atttttaaaa tatcatctta
      121 aatacgccag tcaccaccag tagcccaatc aggaaaataa tcaatacagc ccagagtata
      181 attttcattg gcgttccttt atttgtgagt gctgaataag tataggaaaa aacgaagccc
      241 tttgccgtta tccataaacg attaagataa atcggaagat atcatttact tagtctatat
      301 ttttctggat atgtcgtttt attgctactg atgtattgcc acgcgaaact aaaaaatata
      361 tcgcaacaca cggtaatctc cgcgcacctt ttcttacctg attattcccc catgaatcgt
      421 gattcctttt atccagccat cgcctgtttt ccgctgttac tgatgctggc cgggtgtgca
      481 cctatgcatg aaacccgcca ggcgttaagc cagcaaacgc ctgctgcaca agttgacacc
      541 gcattaccca cggcgctgaa aaatggttgg ccagacagcc aatggtggct ggagtatcac
      601 gataatcaac tcacttcctt aattaacaat gcactacaga acgcaccgga tatgcaggtg
      661 gctgaacagc gcattcagct agctgaagcc caggcgaagg caattgctac tcaggatggt
      721 ccgcagatcg acttttctgc ggatatggag cggcaaaaaa tgtcggcaga aggcttaatg
      781 gggccgtttg ctctgaacga tccggccgca ggtacgaccg gtccgtggta caccaacgga
      841 acttttggct taacggcggg ctggcatctc gatatctggg gaaagaatcg ggcggaggtt
      901 actgcccgcc tgggtacggt taaagcacgg gcggcggaac gcgagcaaac ccgccaattg
      961 ctggctggca gcgtagcccg cctgtactgg gagtggcaaa cccaggcggc gttaaacacg
     1021 gtcttgcagc aaatagaaaa agagcagaac accattatcg cgaccgatcg ccagctatat
     1081 cagaacggga ttacttcttc agttgaaggt gtggaaaccg atattaatgc cagcaaaacc
     1141 cggcagcagc tcaacgatgt cgcggggaaa atgaaaatta ttgaggcacg gttaagcgca
     1201 cttacaaata accagacaaa gtcattgaag cttaaaccgg tcgcgttgcc gaaagtggca
     1261 agccagcttc ctgatgaact ggggtactcc ttactggccc ggcgggcaga tttgcaggcg
     1321 gcgcactggt acgttgagtc atcgctaagc accattgatg cggcaaaagc ggcattttat
     1381 cctgacatca acctgatggc cttcctgcaa caggatgcgt tgcacttaag cgatctgttc
     1441 cgtcattccg cgcagcaaat gggcgttacg gcaggcctga cgctacccat tttcgatagt
     1501 ggtcgtctta acgccaatct cgatatcgca aaagccgaaa gcaacttgtc tatcgccagc
     1561 tacaacaaag cggtggttga agcgctgaat gacgtggcgc gggcagccag tcaggttcag
     1621 acacttgcgg agaaaaacca gcatcaggcg caaattgagc gcgatgcctt gcgtgtggta
     1681 ggtcttgcgc aggcgcgctt taacgcgggc atcattgctg gttcccgcgt cagcgaagcc
     1741 agaatccccg cgctgcgtga gcgggccaat ggcctgttat tgcaagggca gtggctggat
     1801 gcttccattc aactcactgg tgcgttgggc gggggttaca aacgctgatg atatgcgcct
     1861 tctatactta acgtttattc agcgttaagt ggagaactcg atggcacagg ttgcgattat
     1921 taccgcctcc gattcgggga tcggcaaaga gtgcgcgtta ttactggcgc agcaggggtt
     1981 tgatattggc attacctggc actcagatga agaaggggca aaagataccg cgcgtgaggt
     2041 agttagccac ggcgtacgtg cggagatcgt gcagctggat ctcggtaaac tgccagaagg
     2101 tgcgcaggcg ctggagaaac tcattcaacg gctggggcgt attgatgtgc tggtgaataa
     2161 tgcgggcgca atgaccaaag cgccgtttct tgatatggct ttcgatgagt ggcgcaagat
     2221 ttttaccgtt gatgtcgatg gtgcattttt atgctcacaa attgcggccc gccagatggt
     2281 gaaacaaggg cagggcggtc gcatcatcaa cattacgtcg gtacatgaac atacgccgct
     2341 gccggatgcc agcgcttaca cagccgctaa acatgcgctc ggcgggttaa ccaaaacgat
     2401 ggcgctggag ctggtcaggc ataagatttt ggtgaacgca gtcgcgcctg gggcgatcgc
     2461 cacgccaatg aatggcatgg atgacagcga cgtgaagccc gacgcggagc cttcgattcc
     2521 tctgcggcgt tttggcgcaa cgcatgagat tgccagcctg gtagcgtggc tgtgctcaga
     2581 gggcgcaaat tacaccaccg ggcagtcgtt gatagtggat ggcggcttta tgttggcgaa
     2641 tccgcagttc aacccagaat agcgcagtga ggtgcgtggt tatttcaatg ggtaaaattt
     2701 tggtaggcat gataagacgc acaagcgtcg catcaggcat ggtgcaccgg atgtcggatg
     2761 cggcgcgaac gccttaccca gggcaatggt tttacgcctg atgatccggc tttttcttcc
     2821 cgcgccgttt cagccaccag cgcacgccca ccaccagaac cacgaccaga atcaaccaga
     2881 cccagtgctt caaatgctgg tcgagattgt gcaaccacgg cgcaatcacc tgaccaccag
     2941 cgtaaccaat agtggtaaaa atcaacgccc aggcaaatgc gccgagaata ttcagcggca
     3001 gaaagatttt cggcggcagc tggctggcac caatcagcgt cgggccaatc acccgaaagc
     3061 catacataaa gcgcgtacca atgacaaaca gatacggatg gcgttggata agtttctgcg
     3121 cccgctcaat tttatcctga tgtttcgaga aacggcgtaa cagcttgccg ccaaaccgcc
     3181 gcccgcacag atagagcacc tggtcgccaa tcatgccgcc aagcgccaca gaaagtacca
     3241 ccagcgggaa ctttaatagc ccctgatgcg ccgcaacgcc tcccagcaaa gtcacggttt
     3301 caccttccgc caggctaccg atcaccagcg cggcataacc atattgtgag ataagtgtat
     3361 tgagatccat aggccagtct gtctcccttt ggttgccagt aagcatacac cctgtgaatc
     3421 attcacgctg aaaaaggacg ttcatcagac agtgaaaatt acatgggcga acaaaaaata
     3481 atcacaagct gacttatact tgaactgtca ggttcatgaa ctcacaacaa gggggcggta
     3541 tgagccatgt ctggggactg ttttcccatc ccgatcgtga aatgcaggtg attaatcgcg
     3601 aaaacgaaac gatttctcac cattacaccc accacgtttt gctgatggcg gcgattccgg
     3661 tgatttgcgc cttcattggc actacacaga ttggctggaa ttttggcgat ggcactatcc
     3721 tgaagttatc ctggtttacc ggactggcgc tggctgtctt gttttacggc gttatgctgg
     3781 ctggggtcgc ggtgatgggg cgggtcatct ggtggatggc gcgtaattat ccgcagcgtc
     3841 cgtcacttgc gcactgcatg gtcttcgcgg gctacgttgc aactccgctg tttttaagcg
     3901 gtctggtggc gctttatcca ctggtctggt tgtgtgcgct ggtcggcacg gtggcgctgt
     3961 tttacaccgg ctacctgctt taccttggta ttccgagctt cctgaatatc aacaaagagg
     4021 aaggccttag cttctctagt tcgacgctgg cgattggcgt gctggtactg gaagtgctgc
     4081 tcgccctaac cgttatacta tggggctacg gttatcgttt gttctagaaa gttctctgac
     4141 gttgcattgc tggcgtaaat gtcagcaatg cagcatttcc tcacgattct cctttgacga
     4201 tcgcactttt tgctcgttat gatggcgtcg ctagcctcag taaatcctta ctccggcggt
     4261 gcgcaacccg tgcgcgtgaa ccactatctg aatgctcatc atgccgaaat ttcgagtttc
     4321 tttatttagc ctggccctga tgctggctgt gccttttgca ccgcaggccg ttgcgaaaac
     4381 ggcagccgct accaccgctt cacaaccgga aattgcctcc ggtagcgcga tgattgttga
     4441 tctgaatacc aacaaagtga tctattcgaa ccacccggat ctggtgcgtc cgattgcgtc
     4501 tatcagcaaa ttaatgaccg cgatggttgt gctggatgca cgactgccgc tggatgaaaa
     4561 actaaaagtg gatatcagcc agacgccgga gatgaaaggg gtctattcgc gcgtacgact
     4621 gaatagcgaa atcagccgta aagatatgct gttgctggcg ctgatgtctt cagaaaaccg
     4681 cgcggcggca agccttgcac accattatcc cggtggttac aaagccttta ttaaggcaat
     4741 gaatgcgaaa gcgaaatcgc tcggaatgaa caacacgcgc tttgttgaac ctaccggatt
     4801 gtcggtgcat aacgtttcaa ctgcccgtga cttaaccaaa ctgctcattg ccagcaaaca
     4861 atatccgttg atcgggcagt taagtactac ccgggaagat atggcaacct tctctaaccc
     4921 gacgtatacg ctgccgttcc gcaatactaa tcatctggtg tatcgcgata actggaatat
     4981 tcagttaacc aaaaccggct ttaccaatgc ggcgggccat tgtctggtga tgcgtacggt
     5041 tatcaataat aaaccggtgg cgctggtagt gatggacgcg tttggcaaat atacccattt
     5101 tgccgatgcc agccgcctgc gtacctggat tgaaaccggt aaagtgatgc ctgtgccggc
     5161 agcagcgttg agctataaaa aacaaaaagc cgcccaaatg gcggcggcgg ggcagacggc
     5221 acagaacgat taagtgaatt cggatggcga tactctgcca tccgtaattt ttactccact
     5281 tcctgccagt ttttccgttt actggtttta ccgatccccg gattcatgct gttggtcgga
     5341 tcgttctcgc gatagaactt ctgcaacgtc tccggtgctt tatacaaatg accgacgtta
     5401 tgctcggcag ggtattgcgc gccgcgctgc tgtagcagtt ccagcatctg ttcttttaac
     5461 gcatgcacat ccacgccttt tttcactatg taatcctgat ggaagacata gcacataaaa
     5521 tggccgtaat agagcttgtg caccagctgg ctgtcgatct ccggcggtaa atgctcatac
     5581 cactcggtgt cgttacgccg cagagcgata tccaacgcca gaatgtcttc gacttcatcg
     5641 gaatgcaccg cctgataacg aattgctgcg cccgcagcgg cgaaacggtg taaaaacgct
     5701 ttgctgcctt cctccggcgt acagacaaag aaatcgcctt cggcctgttt gaaataatcc
     5761 accaaccacg atttggcttc gcccacgcca tcgcctgcca tttttaacag cagatgatgc
     5821 tcgtatttat cgcgccagtt tttcatgcgc ggcggtaaat ggctggggaa caggtgaccg
     5881 aatttttgca tcgcacggtc agtaaaatgc ggacggaaga atttcacttt ctccagcatc
     5941 gcatcggtgc gtcccttgag attaaagaag aacggcatct tgtcggtgcc taacttatca
     6001 atcatcagga aggtgtcttt gccgtatttt tccgcaatat cgtagatatc ccggtgcata
     6061 tattccccgg caaccggcag attttcgaag ttagccagaa tatgacggcg gatttcggtc
     6121 agcacttccg gctggttggt gccgatataa aacacctgct gatttttttc cgcttcgaag
     6181 gtatctagac gtactgcaaa gaccgccagc ttcccggcgc aaccagaaga ttcaaataac
     6241 cgatcaggat cggcgttata acgtgcgggc gtgtcggctt caatatcacg aacgcggtgg
     6301 acatagtcat aatcgtgggc gtgacgacca tcgtgacgca catcgtcatc tttgatgcga
     6361 tcatcatcca gcttgctaag gatctgctcc ggcgtttcgc ccagatcaat ccccagatgg
     6421 ttcaccagcg tcagtttgcc gtcttcattt atacgcgcga ataacgacat ttcggtatac
     6481 gccgggccac gttgcaccag cgagccgccg gagttgttac aaataccgcc gataaccgat
     6541 gcgcctatac acgacgatcc aatcaccgag tgcggttcgc gtcccagcgg tttgagggct
     6601 ttttccagcg aatagagcgt ggtgcccgga taggccagca cctgttcgcc cttgccaaga
     6661 acgtgcagct tgtcgagacg cagggtgctg atgatcacga tatcgcgatc atagtcgtta
     6721 ccgtttggcg tcgatccttc ggtcaggcct gtattggcag cctgcatcag aataattttg
     6781 tcggcggtga cacaggcttt cagcacccgc cacaattcca gtagtgagcc agggaaaacg
     6841 acagccagcg cgtcgccctg accagaacgg aagcccttgc gatagcgggc cgtttttgcg
     6901 ggatcggtga gcaggtgtga atgaccgacc agacgggcaa gttcattcaa aaaggcttta
     6961 ttatcagttg ttgtcatgga agacattttc cactccttgt ggtggcgaaa aaatatcaca
     7021 aactagaata gcgccttgta tgacaattca gcgagtgaat attttgaaaa ttcagacaat
     7081 aaactgagca ttctttttct ctctccatca tgcttatggc aaactacggt ttttgcacca
     7141 ttttcgctgc ctcgtcgggc aacagaggaa gaaaaatcca tatgaaatgg ctatgttcag
     7201 taggaatcgc ggtgagtctg gccctgcagc cagcactggc ggatgattta ttcggcaacc
     7261 atccattaac gcccgaagcg cgggatgcgt tcgtcaccga actgcttaag aaaatgacag
     7321 ttgatgagaa aattggtcag ctgcgcttaa tcagcgtcgg cccggataac ccgaaagagg
     7381 cgatccgcga gatgatcaaa gacggtcagg ttggggcgat tttcaacacc gtaacccgtc
     7441 aggatatccg cgccatgcag gatcaggtga tggaattaag ccgcctgaaa attcctcttt
     7501 tctttgctta cgacgtgctg cacggtcagc gcacggtgtt cccgattagc ctcggtctgg
     7561 cctcgtcttt taacctcgat gcagtgaaaa cggtcggacg tgtctctgct tatgaagcgg
     7621 cagatgatgg cctgaatatg acctgggcac cgatggtcga tgtctcgcgc gatccgcgct
     7681 ggggacgtgc ttccgaaggt tttggcgaag atacgtatct cacctcaaca atgggtaaaa
     7741 ccatggtgga agcgatgcag ggtaaaagcc cggcagatcg ctactcggtg atgaccagcg
     7801 tcaaacactt tgccgcatac ggcgcggtag aaggcggtaa agagtacaac accgtcgata
     7861 tgagtccgca gcgcctgttt aatgattata tgccgccgta caaagcgggg ctggacgcag
     7921 gcagcggcgc ggtgatggtg gcgctgaact cgctgaacgg cacgccagcc acctccgatt
     7981 cctggctgct gaaagatgtt ctgcgcgacc agtggggctt taaaggcatc accgtttccg
     8041 atcacggtgc aatcaaagag ctgattaaac atggcacggc ggcagacccg gaagatgcgg
     8101 tgcgcgtggc gctgaaatcc ggaatcaaca tgagtatgag cgacgagtat tactcgaagt
     8161 atctgcctgg gttgatcaaa tccggcaaag tgacgatgga agagctggac gatgctgccc
     8221 gccatgtact gaacgttaaa tatgatatgg gattgtttaa cgacccatac agccatttgg
     8281 ggccgaaaga gtctgacccg gtggatacca atgccgaaag ccgcctgcac cgtaaagaag
     8341 cgcgtgaagt ggcgcgcgaa agcctggtgt tgctgaaaaa ccgtctcgaa acgttaccgc
     8401 tgaaaaaatc ggccaccatt gcggtggttg ggccactggc ggacagtaaa cgtgacgtga
     8461 tgggcagctg gtccgcagcc ggtgttgccg atcaatccgt gaccgtactg accgggatta
     8521 aaaattccgt cggtgaaaac ggtaaagtgc tgtatgccaa aggggcgaac gttaccagtg
     8581 acaaaggcat tatcgatttc ctgaatcagt atgaagaagc ggtcaaagtc gatccgcgtt
     8641 cgccgcaaga gatgattgat gaagcggtgc agacggcgaa acaatctgat gtggtggtgg
     8701 ctgtagtcgg tgaagcacag gggatggcgc acgaagcctc cagccggacc gatatcacta
     8761 ttccgcaaag ccaacgtgac ttgattgcgg cgctgaaagc caccggtaaa ccgctggtgc
     8821 tggtgctgat gaacgggcgt ccgctggcgc tggtgaaaga agatcagcag gctgatgcga
     8881 ttctggaaac ttggtttgcg gggactgaag gcggtaatgc aattgccgat gtattgtttg
     8941 gcgattacaa cccgtccggc aagctgccaa tgtccttccc gcgttctgtc gggcagatcc
     9001 cggtgtacta cagccatctg aataccggtc gcccgtataa tgccgacaag ccgaacaaat
     9061 acacttcgcg ttattttgat gaagctaacg gggcgttgta tccgttcggc tatgggctga
     9121 gctacaccac tttcaccgtc tctgatgtga aactttctgc gccgaccatg aagcgtgacg
     9181 gcaaagtgac tgccagcgtg caggtgacga acaccggtaa gcgcgagggg gccacggtag
     9241 tgcagatgta cttgcaggat gtgacggctt ccatgagtcg ccctgtgaaa cagctgaaag
     9301 gctttgagaa aatcaccctg aaaccgggcg aaactcagac tgtcagcttc ccgatcgata
     9361 ttgaggcgct gaagttctgg aatcaacaga tgaaatatga cgccgagcct ggcaagttca
     9421 atgtatttat cggcactgat tccgcacgcg ttaagaaagg cgagtttgag ttgctgtaat
     9481 gctttagtaa gggcgcgacg tttgcgccct ttgtaggccg gataaggcgc tcgcgccgca
     9541 tccggcagga aacgccggac cgaatatgcc tcttaagtat ttatctgatt ctgcaactga
     9601 atccttccgc tcaagctaac cccgccatta tcaactatgc ttttctctta attcgctgaa
     9661 aacaacagta aaagaatgag gaaagcatca tgccactctt aaagctctgg gcaggttcac
     9721 tggttatgtt ggcagccgtg agcctgccgc tacaggcggc ttcccccgtt aaagtcggtt
     9781 caaaaatcga taccgaaggt gcgctactcg gcaatatcat tttgcaggtg ctggaaagcc
     9841 acggcgtacc gacggtgaat aaagtgcaac tcggaacgac tcctgtggtg cgcggggcga
     9901 ttacttccgg tgaactggat atctatccgg aatataccgg caatggcgct ttcttcttta
     9961 aagatgaaaa cgatgcagcg tggaaaaacg cgcagcaagg ttacgagaaa gtcaaaaagc
    10021 tcgattcgga gcacaacaag ttaatctggc tgacgcccgc gcctgcaaat aacacctgga
    10081 ctatcgccgt gcgtcaggat gtggcagaga aaaataaact cacttcgctt gccgacctga
    10141 gtcgttatct gcaagagggc ggcaccttca aactggcagc ctcggcagag tttatcgaac
    10201 gcgccgatgc gttacccgcg tttgaaaaag cctacgactt taaactcgat caggatcagt
    10261 tactgtcact ggctggcggc gacacggcgg taacgattaa agccgctgcc cagcaaactt
    10321 ctggcgttaa tgccgcaatg gcttacggca ctgacggtcc ggtcgcggcg ctggggctgc
    10381 aaaccttaag cgatccgcaa ggcgtgcaac ctatctacgc gcctgcacca gtggtgcgtg
    10441 agtcggtgct gaaagagtat ccgcaaatgg cacagtggct acagccagtc ttcgccagcc
    10501 tcgatgcaaa aacattgcag caactgaatg ccagcattgc agtggaagga ctggatgcca
    10561 aaaaagtggc tgccgactac ctgaaacaaa aagggtggac gaagtaattt cccgtgactt
    10621 atctacgtat taatcctgtt ctggcgctgc tgctgttgct gacggtaatc gcagcggcgc
    10681 tgccgtttat cagttacgcg cctaatcgtt tagtctcggg tgaagggcgt catctctggc
    10741 agctgtggcc gcaaacgatc tggatgctgg tgggcgttgg ttgcgcctgg ctgacggcct
    10801 gttttattcc cggtaaaaaa ggcagcattt gtgcactcat tctggcgcaa ttcgtcttcg
    10861 tattgctggt gtggggcgcg ggaaaggcgg cgacacaact ggcgcaaaat ggcagtgcgc
    10921 tggcgcgtac cagcctcggc agtggtttct ggctggctgc ggcgctggca ttgctggcct
    10981 gtagcgatgc catccgccga atctccacgc atccgctgtg gcgctggttg ttgcatatgc
    11041 agattgccat tattccgctg tggttgctgt actccggcac gcttaacgat ctctcactaa
    11101 tgaaagaata cgccaaccgt caggatgtgt ttgacgacgc gctggcacaa catctgacgt
    11161 tgctgtttgg tgcggtgctg cctgcgttag tgattggtgt gccgttgggc atctggtgct
    11221 acttttccac tgctcggcag ggggcaattt tttctctgct caatgtcatt cagaccgtgc
    11281 cttcggtggc gctctttggc ctgttgattg cgccgcttgc cgcgctggtg acggcctttc
    11341 cgtggctggg gaagctcggc atagcaggaa ccggaatgac acccgcactg attgcgctgg
    11401 tgctctatgc cttgctgccg ctggtgcgcg gcgtggtagt cggtttgaac cagatcccgc
    11461 gcgatgtgct ggagagcgcc agagcgatgg gcatgagcgg ggcgcagcga ttcctgcatg
    11521 ttcagttacc actggcgtta ccggtatttc tgcgcagcct gcgggtggtg atggtgcaaa
    11581 ctgtaggtat ggcggtgatt gcggcgttaa tcggcgcagg cggttttggt gcgctggttt
    11641 tccaggggct gctaagcagc gccattgatt tagtgttgct gggggtgatc ccggtaattg
    11701 ttctggcggt gcttaccgac gcgctgttcg atttgcttat cgcactgctg aaggtgaaac
    11761 gtaatgattg aatttagcca tgtcagcaaa ctgttcggcg cacaaaaagc cgttaacgat
    11821 ctcaatctca attttcagga agggagtttt tcggtgctga ttggcacatc tggctccggc
    11881 aaatccacca ccctgaaaat gattaaccgc ctggtggagc atgacagcag agtgatccgc
    11941 tttgccggag aagaaattcg ctcgctgcca gtactggagt tgcgccgccg gatgggctat
    12001 gccattcaat ctattggcct gttcccccac tggagcgtgg cgcaaaacat cgctaccgtg
    12061 ccgcaattac aaaaatggtc gcgggcgcgg attgacgatc gtatcgacga attaatggcg
    12121 ctactggggc tggagtcaaa tttacgtgag cgttatccgc atcagctttc cggtggtcag
    12181 cagcaacgtg tgggagtggc gcgcgcactg gctgccgatc cgcaagtctt actgatggat
    12241 gaaccttttg gcgcactgga cccggtaacg cgcggcgcgt tgcaacaaga gatgacgcgc
    12301 attcaccgtt tgctggggcg taccattgtg ctggtcactc atgatattga tgaggcgcta
    12361 cggctggcag aacatctggt attgatggat cacggtgaag tggtgcagca gggcaatccg
    12421 ctgacgatgc tgactcgtcc ggcaaacgat tttgtccgcc agttttttgg acgtagtgaa
    12481 ctgggggtgc gcctgctttc gttacgtagt gtggcggatt acgtgcgtcg cgaagaacgg
    12541 gcagaaggtg aggcactggc agaagagatg acgctacgcg atgcgctctc cctgtttgtc
    12601 gcgcggggat gcgaggtgct gccggtgatg aacacgcagg gccagccttg cggcacgctg
    12661 cattttcagg atctgctgga ggaggcgtaa gcgtatgaaa atgttgcgcg atccgctgtt
    12721 ctggctcatt gctttgtttg tggcactgat tttctggctg ccttacagcc agccgctgtt
    12781 tgctgccttg ttcccacaac tgccacgacc cgtttatcag caagaaagtt ttgcagctct
    12841 ggcactggct catttctggc tggtgggaat ttcgagtttg tttgcggtga tcattggtac
    12901 tggtgccgga attgctgtca ctcgcccgtg gggcgcggaa tttcgcccac tggtggaaac
    12961 tattgccgcc gttggacaga cttttccgcc cgtcgcagtg ctggcgatcg ccgttccggt
    13021 gatcggcttt ggtctgcaac cagcgattat cgccttgatc ctttacggtg tgctgcccgt
    13081 cctgcaggcg acacttgccg ggctgggagc gattgatgcc agcgtgacag aagttgcgaa
    13141 aggtatggga atgagtcgtg gtcagcgact gcgtaaggtc gagctaccgc tggcggctcc
    13201 ggtgattctg gcgggcgtgc gaacttcggt gattatcaac attggtacgg cgacgatcgc
    13261 ctcaacggta ggggccagca cgctgggtac gcccatcatc atcgggctta gcggatttaa
    13321 taccgcgtat gtgatccagg gggcgttact ggtggcactg gcggcgatca tcgcagaccg
    13381 cctgtttgaa aggctggtgc aggcgcttag ccagcacgca aaataaaggt ataacctgcg
    13441 agcatgacgc caccaattcc gcctaacgcc ataaacagga acagggcaat gaccccaatt
    13501 ttagctatgc gcataatgca ctccttatgt taacgaaagg attgtacagt aaagcgcatt
    13561 tgttaacgaa tcattaaatg ccgagtggga aaatatcatg gccttgttcc tgccaactgg
    13621 tgagttgttg ctgttgggcg gaggttcgat tttcaccgca ccacaccagc aatgtacggc
    13681 cttcgaatag ttcagggcgt agttgattga gcgagtgggc gaggacatca atgcgccatc
    13741 cttgttgact ggcaatccag ccctccagcc acagacgggt ggtatcctga atattccagc
    13801 caaccaccag cgcatcttta ccctgttttt tacgtgccga agccagacaa atggcgatgt
    13861 agttgatcag tacgccgtcg aggatcgcca gcagcgcctg gagagtcggt tgttggcatt
    13921 gaagtcgtcg gcgcagagga ataaacaggt gcgtggtgag tgtctgggcg gggtaatcct
    13981 gaccgcgctc tttgatccac gttcgcaggc tatgcagatt gccgctttgc aggtaggtca
    14041 gtaatgtttc ttgctgatcg cgccagccgt tctgcacatc aacattttca ttactgagca
    14101 gcattttaac tttgctgacc tgcacgccgt tgtcgatcca gcgtttgatc tcgcggatcc
    14161 ggtcaatatc ggcatcgttg aacagtcgat gaccgccgtc tgtccgttgc ggtttcagca
    14221 acccgtaacg cctctgccac gcgcgtaacg tgacagggtt aatatcacaa agcaacgcca
    14281 cttcaccaat tgtgtaaagc gccatcgtct cacccttgct cgcgaggtcc cagtttaact
    14341 ttagacgcag ttttgcgaac caggtagttt tgcccgtttt ttgtgcatct atagggtgat
    14401 tttatttttg ccaggcgatt ttgagtgatc gtactcacga attctcattt ttctgcaaga
    14461 gttcaaagaa agttaaacgc aggcaatgta tgttacgcgt tttaaaggga agtgtggttt
    14521 gcgggtatgt acgattttaa tctggtgttg ctgctgcttc agcagatgtg cgttttttta
    14581 gtcattgcgt ggttaatgag taaaacgcca ttattcatac cgttaatgca ggtcacggtt
    14641 cgtctgccgc ataaatttct ctgctacatc gtcttttcca tcttctgcat catgggcacc
    14701 tggtttgggt tgcacattga cgattctatt gccaataccc gtgcgatagg cgcggtaatg
    14761 ggcggcttac tcggcggtcc ggtcgtcggt gggctggttg gtctgaccgg cggcttacat
    14821 cgatattcga tggggggcat gaccgcgcta agttgcatga tctcaaccat cgttgaagga
    14881 ttgctcggcg gcctggtaca cagcatcctg atccgtcgcg ggcgcactga taaagtcttt
    14941 aaccccatta ccgccggtgc cgtcacgttc gtcgctgaaa tggtgcaaat gctgatcatc
    15001 cttgcgatcg cccgacctta tgaagatgcg gtgcgtctgg tgagtaatat tgctgcgcca
    15061 atgatggtca ccaataccgt cggcgcggcg ctgtttatgc gtatattgct cgataaacgc
    15121 gcgatgtttg aaaaatacac ttcggctttt tctgccactg cgctgaaagt ggcagcctcg
    15181 acggaaggca ttttgcgaca ggggtttaac gaagtgaaca gcatgaaagt ggctcaggtg
    15241 ctgtatcagg agctggatat tggtgcagtc gcgattaccg atcgagagaa attgctggcc
    15301 tttaccggaa ttggtgacga ccaccattta cccggcaaac cgatttcttc gacttacacc
    15361 ttaaaagcga ttgaaaccgg tgaagtggtc tacgctgatg gcaacgaagt accttatcgt
    15421 tgctctttgc atccgcaatg caaactgggg tcgacgctgg taattccgtt gcgtggcgaa
    15481 aatcagcgcg tgatgggcac catcaaattg tatgaagcca aaaaccgttt attcagttca
    15541 ataaaccgca cgctgggcga ggggattgcg caactgcttt cggcgcagat tcttgccggg
    15601 caatatgagc ggcaaaaagc gatgctcacc cagtcagaaa tcaaactgct tcacgcccag
    15661 gtgaatcctc attttttgtt taatgcgctt aacaccatta aagcggtgat ccgccgcgac
    15721 agcgaacagg ccagccagct ggtgcagtat ctttccactt ttttccgcaa aaacttaaag
    15781 cggccttcgg agtttgttac tctcgccgac gaaattgaac atgtgaacgc ttatctgcaa
    15841 attgaaaagg cgcgcttcca gtcgcggttg caggtcaaca ttgctattcc gcaagaatta
    15901 tcccagcagc aattgcccgc gtttaccctg caaccgatag tggaaaacgc cattaaacat
    15961 gggacatcac aactgctgga tacagggcga gtggcaatca gcgcccgacg tgaggggcaa
    16021 catttgatgc tggagatcga agacaatgcc ggtttgtatc aaccggtaac caatgccagt
    16081 gggctgggga tgaatctggt ggataagcgt ttacgtgaac ggtttggcga tgactatggg
    16141 ataagcgtcg cctgtgagcc tgatagttac acccgaataa cgttacgact accatggagg
    16201 gacgaggcat gattaaagtc ttaattgtcg atgatgaacc gttagcacgg gagaacctgc
    16261 gcgtattttt gcaggagcag agcgatattg aaatcgttgg agagtgttca aacgccgtgg
    16321 aagggatcgg cgcggtgcat aaactgcgcc cggatgtgct gtttctcgat atccagatgc
    16381 cgcgcatcag tggtctggaa atggtgggga tgcttgaccc ggaacatcgc ccgtatattg
    16441 tttttctcac cgcgtttgac gaatacgcca ttaaagcctt tgaagaacac gcctttgatt
    16501 atctgctgaa gccaattgat gaagcgcgac tggagaaaac gctggcgcgt ttgcggcagg
    16561 aacgcagcaa gcaggatgtt tcgctgttac cagaaaatca acaggcgctg aaatttatcc
    16621 cttgtactgg gcatagtcgg atttatttgc tgcaaatgaa agatgtggca tttgtcagca
    16681 gtcggatgag cggtgtctac gttaccagcc acgaagggaa agagggcttt accgaattga
    16741 cattacgtac cctggaaagt cgtacaccac tactgcgctg ccatcgtcag tatctggtta
    16801 acctcgcgca tttacaggag attcgtctgg aagataacgg ccaggccgag ttgattttgc
    16861 gtaatggctt aaccgtgccg gtcagccgcc gttatctgaa aagcttaaaa gaggcgattg
    16921 gcctgtaaaa gactgctaaa atggcttttt gcctcatcaa cacctgaagg cctcatgcta
    16981 agtaacgata ttctgcgcag cgtgcgctac attttgaaag ccaataataa tgacctggtg
    17041 cgtattctgg cgctgggtaa tgtcgaagcc accgcggaac agatcgccgt ctggctacgt
    17101 aaagaagacg aagagggttt tcagcgttgt ccggacattg ttttgtcgtc attcctcaat
    17161 ggcctgattt atgaaaaacg cggcaaggat gagtctgctc cggcactgga gccggaacgt
    17221 cgcattaata acaacatcgt gctgaaaaaa ttacgcatcg cgttttcgct gaaaaccgat
    17281 gacattctgg ctatcctcac cgaacagcag ttccgcgttt cgatgccgga aattacagcg
    17341 atgatgcgcg caccggatca taaaaacttc cgcgaatgcg gcgatcaatt tttacgttat
    17401 tttctgcgtg gactggcagc gcgccagcat gtgaagaaaa gctaagacgg gtatggcggc
    17461 catgcgaaac atggccgccg acagattatt tcacttcttt aaaaccagcg gctttcatca
    17521 ccagttccat ttgcgccata gtgatacctt ttttggcatc ttcagcagaa acgttgattc
    17581 ctgaaatacc ctgcagggct ttaaaatcca ctttttccat atcgatagtc acgttttcct
    17641 gcgcgtaggt atctgtatag gttaattttt cttcaacacc cgcgatgttt ttgtatttgg
    17701 cgcttaacgg ctcaagtgtc ttggcagcgt cttctttggt ggttgcacca atagaagcaa
    17761 attgaatttt ggtttcagaa gattgcttaa gcaccttgtc acctttgtag acataggtaa
    17821 tggcaatttc agtgccgttc agattggcgc tgaatttctt cgattcttct ttgtcaccgc
    17881 agccagcaag agagaaaacc agaacagatg caacaacgag ggaaaacagc ttattgaaag
    17941 ccttcatgta aaactccatt ttatttaatc aagaaactgg tgactctcac caggggctat
    18001 atagaatatg cctaataccg tgacgtgagc agtccggaac tggagtagaa ctcttagtaa
    18061 aaagcactat ttcatccttg ttgctgaagc atggggaata attgttcgca aagtaaaaca
    18121 ccgttattca ttgcttctac ccgtgcctcg ctttctgtat tacgaaattg tcccaacaca
    18181 tgtgccagcc gataaaaacc gaccgcggag aggtcattcg ccagcaactc tgcctgacta
    18241 atagcgctct gttcctgata gcgccagccg ttatggagca gttgaataag taacgcctgg
    18301 cagcgcatca gcaactgatg agcggtagac ggcacaggca aaacgctggc agaaggtagc
    18361 ggtgccacag gcgcagtttc tgcgtccagc gcccaggcgc gggtttttgt catcatcacc
    18421 cgtggttcca gtgtcaattg cccatcaaca aaactgacaa agccagaaac cagactcacg
    18481 gggtcgtctg tttgttgcaa aagcgccgcc atgcgttcaa cggcaaaagg agaacaggca
    18541 gatgccggaa gggataacgt caggagatta tcttcacctt cgccgctgat tacctgcgca
    18601 tccagcgtct ggcggctgct atcccaaccg agcgaaatac actcagcgac cggcagaata
    18661 aataagttat cgacctgatt aagaggccgt atgcaggcgg ggggacgctg gcgtaaatat
    18721 tcccgcaaag ccacaatgcc cggctggcgt aacggcgcgc tcaacatttg ccaggcatca
    18781 ggcgacagcg gcacaacgct gcttaagcgg ttgcgggtag ctaacagcag ctcgccatcg
    18841 gcactgcgtt ttgctgcttg tgaaacaatt tgcccacccg ccagtgcgcc agcctgaaaa
    18901 ctaaacagcc gacgcgtagc tgccggtgag ttttcctgtt cacttcgcgg ccaactgcgc
    18961 gaaaggtgca aaatactgcc ggtgtcggga tcggtaaacc agatgcgtaa accataatgc
    19021 tcaatatcct gccagcaacg catacctaaa gacaccagcc gcagatgatc aagctttgct
    19081 tctccggcaa tgccagagcc aacgaccgtg cgccacggca caggaggaac ttcaccaaca
    19141 ctgtcgcgcc gggccatctc ttgtgcgcaa tttaatcgac tgtttaatgc cgcaagctga
    19201 cgtaagcatt ctccggcatg atagtggctg gcgcgggcgt ggaaggcatc aacgcttgcc
    19261 cgtagctgtc gtagtgattc actcacccat cgccagttgc agcgttccgc cgcctgctgc
    19321 gcgcgactga aagcggcctc gtagtgaata agcggctggc tgatgccgcc cagccataat
    19381 gcctggctta attgctgaac atattgacga cacgcgttac cctcgtcgtt ggcaaacgga
    19441 tcgtcagatg atgtgacgtg ttcgctgcgc atctgccaga ttaaatgagt aaattccgct
    19501 tgctgagttt tggcctcgac gaaggcctgc acagccagta cgacatgttc gcaaagtgtg
    19561 ccttcaatac aatcacaacg ggcgaaacga atactgctgc gggaataaaa acgcacatcg
    19621 ctcatcggta agcgggcaga gggaatttcg cccggcgtac agaacaactc aatggtgatg
    19681 cctttagcga ccaacgcctg tgcgcgtttg cgggtggcat cgggcagggt agccagttct
    19741 tccagccaga ttgccggatc ccacgcttct tctttttccg taggctgggc ggtagtacaa
    19801 agtcgttgat aacttaacac aagcatcacg cgatgacggc acataccgct ggccccgcag
    19861 ctgcactgag cctctttcag tgcctggctg ttcgccagct gggtacggac accgtcactg
    19921 aaggtggcga ttaaagcgct gttctcatgg ctgatttccg ggacgttgcc attttccagt
    19981 tccttaagac tgcgcttaac aaaaccggca ttgcttaacg ccgtcagggc ctgcggtgtc
    20041 agttctaata attccggacg tagtgaattc atgactgaag attctccgca agccatgatg
    20101 ccagctcgcc cggtgtcatg gcggctattt gtgcgccgac atttaccagc gcctgggccg
    20161 tatcgtggtc atagcaaggt gttgctgtgc tatcgagcgc tgccagtccc agcactttga
    20221 tgccgctctg gacacacttt ttcacctgat gcgtcagtaa tgatgatgaa cccccttcgt
    20281 aaaaatcgct cacgaggata atgacgcttt tcgctggttg ttcaataagt tgccgaccat
    20341 actccacggc actggcgata ttggtcccgc cgcccaactg tactttcatt aataactcta
    20401 ccggatcggc aacgtctgcc gtgagatcaa cgacgcttgt gtcaaacgcc accagatggg
    20461 tacgaatgcc gggtaactgc cacaaacagg ccgccatcac cgcagagtgg atcaccgaat
    20521 cgaccatcga tccgctttga tcaaccagta agaccagttg ccattgttcg ctttggcgtt
    20581 taatgcggct gttaaagcgg ggggattcga tatacaactt gccgtgttgc gggtgccagt
    20641 gttgcaggtt ggcgcgcaga gtacttttga aatcaaagtt tcgcgccagt ggaataaatg
    20701 aacggcgacg gcgatcgcgg acaccagaaa aagcctggcg aacttccttt gccagtcgcg
    20761 ccataatttc ttcaacaacc tggcgcacta tctggcgggc ggcagccagt acttcgggat
    20821 tcatcagatg tttggtgtgc aaaacggcgc gtagcaggct ttcggaaggc tgcatacgtt
    20881 ccagcacgtc gagatttgtc accacatctt caatgccgta gcgcagtacg gcatcgcttt
    20941 ccagccgctc aatcacctgt tgcggaaaca gcgtgtggat actgttgatc cactcaggag
    21001 tggtgagatt tgagccacct aatccaccag agcgttcacc acgctggagc cgttcaggat
    21061 cgcgcccata cagccactcc agcgcgtgat ctatctgccg ggcgttgtca tccagcccac
    21121 aaagcgtcgt ttctgccgct tcgccaagaa ttaatcgcca gcgttgtagc tcacgggtgg
    21181 tcagaagatc gttcagttca gacatggaaa actccccagt gttgcagcga tgccagtgcc
    21241 tgctgttcga gttgttgatg atgtgcaatg gcttgcggtg gacaatgcaa cggcatttgc
    21301 agtgccgaaa cgggaagttg cgccagttga taatgctcaa gcacctgatg cgccagcgtt
    21361 ccgcgttctc gtggcggtag ccaggccatc gccgcgcgta aatcgggcag ggcattgata
    21421 aaatcggcat cacttagttg atttaaatga ctgctgaaac cggcgataaa tgtcggctga
    21481 caggccagtt gatgacgggc taatgccagc aaaccttgca gcgcctcgcc ggattgtgcc
    21541 ggggataact gcgccagcat cgtcagcgcc gcttcggcac tggcgttggg atgttccaga
    21601 cgcattagtg cgccaagaac cgcgccgcga tccagcgccg gggcatgaat cgcctgactg
    21661 cgtcgctcca gtaaggctac cgccgcagaa agcgaaacgc cggataaatt aacgccgcta
    21721 tgtagatcgc gcagaatatg gcaaagcgct tgccagctat gcaggtgagc gtgaaactcc
    21781 ttttcatccg gtctgccgtt agattcacac agccacagcg tgcgatcgat agccgcgcat
    21841 aacgtcgttt gtaatatctg cgcgccttgc ataccgctaa tttcatccag ccgccataag
    21901 gcatatagca cttccagcgc ctggcccatt tcggcaaatt gattttcctg ggcgattaat
    21961 aatgtgagtt gctccagtaa ttgttgactg aaggacgcca gacccgctaa cgccgcctgg
    22021 ctaagacatg tggtgatact gccgattcca cccgcgtcca gcatatcggc ttctaattta
    22081 tggcgtgcgg cttcctggag tgtggcacca aagcaggcgg cctcaatcaa tgccgcatgc
    22141 tggctaagcg ggcgggttaa tttccagtgt tcttcaccgt tgcctgcaag tgtcagtgtg
    22201 cttccctgct ggcgcacaat ccctgggatc tccagtattg ccagccgatg taacacctga
    22261 ctttgcgcta gtccattggg gttaaagcga tttaaggtaa gctccgccgg aagggagatt
    22321 gcggtacgtt ccagttcgca ggtgacatct tttggcagag gaggttgtgg cgtagaaggg
    22381 gcaagtttac cgaatccgtc acccgctaat gtgtctatca acgttagcag aatcggatcg
    22441 gtgtcgggat gaataacgcc gcgatagctc cacggcaacg gcgcattcag ggcttcttta
    22501 atcagcgatc ctgctatggc atccagccag tcagtgcgta acggtagtgt atgaccgcgc
    22561 aactgtgcca gcgccatcgc atgcagatga gcggcagcca tatccgcggt cgaagcaggc
    22621 aacttgtgct ggcgcaaacg ggtaaggata gtttttagca gttgttcacc ggcctgctgt
    22681 aagccccact gccagcacca gttttgccag accggggcag gcataccgga aaggtatcct
    22741 gccagcacat caaggcgctt ttcactgtag ggcgtgagat aacaacctgt aatggcatct
    22801 gccagcgagg gcaattccgg cgtgttaatg tcctgcgggc attcgcgcca catttttgcc
    22861 agtgccggag cgtgccagcc accgcagacg actaacacgt cgccattatt ttgctgcacc
    22921 gcccatgcaa tccagcgagc cataaaggct tcgcgctgac gattgagtgc atcgccgggg
    22981 aaatcgcccc gcagttgggc aaaatagtgc gccagcgcag agggtaatgc agtttgctgg
    23041 ctttcatctt cgaacaagtg atcccacagg gtatcgctgt tatccatgcg ggtggcacgc
    23101 agcagtaagg cctggctttc atcttgcgta tcaggcgaat cgtcctcttc ttcactctgc
    23161 gcccagcaag gcaaatcgat gaagtaagtt tgtgcctgaa tacgacgtgc ggcttgtagc
    23221 gcctgccact ccggcgaaaa ttcagcaaat ggcgtccagg caccacgccc gggggctgca
    23281 ccgtcctgat actggcaata actgtaaata gctaccggaa gctggtggga aaaaaaaagt
    23341 tcgtctaccc ggtcattaaa atcagccggg ccttcaatca acacgtatcg tggccgctgg
    23401 ctttcgatta acgatttcac cagccgggcg caggccggac tatgatgccg gatgccgaca
    23461 attaacggct cgctcatgcc tctttcctca cggcaggcgg tggcgggctt gataataagc
    23521 ctgccagtga gcttctttat gtgtggcgac acgctgttcg aagtaacggc gcagacgagc
    23581 gcgatcttct tcgttatctt tgacgatggt tccggcaata cagtccacca ggtcggcagg
    23641 ctcccccgcg cgattcgcta aaaaccaggc gcggacgcct acggcgtggg ccacgttcac
    23701 ggcttcagcg gtggacataa tcgctgtcag tgtatccatt gacgttttct tttcgccgtt
    23761 ggcacgcaga tcgcggaagg tgcgtaccag taactccagt actgcgtcag gcactttatg
    23821 cggaataccg ctatgggcca gcaaacgcgc cgaagcactg gcgaccagtt ccagctcctg
    23881 ggcgaaatcc ataatcggaa agacggtttc aaaatcgaag cgacgcttta gcgcggcact
    23941 catctcattg acgccgcgat cgcgggtatt ggcagtggca ataatattaa agccttcccg
    24001 cgcatagagc tgactggctt caccggtgag ttccggcacc gtcatcaccc tgtcagaaag
    24061 catacctaac agacagtctt gtacttccag cggtgtacgc gttatctctt caaaacggac
    24121 gattttgccg tcgcgcattc cctggtaaag cggggcaggg accagtgctt ccgttgacgg
    24181 accatggttg ataagcaggg cgtagttcca gccgtacttg atttgatctt cggtagtaga
    24241 tgcgccgccc tggatggtta acccggcatc gccgctaata gcggttgcca gtaattcaga
    24301 caggagagat tttgcggtgc caggttcccc caccaacatc aaaccgcgcc cggtggcaag
    24361 agtgactaac atgcgttcga cagcggaggg actaatcacc actttacggc taatattttg
    24421 cgcgctatcg ccaaggataa aagcacgggc cgcaggcaaa cttaactgcc aaccgggcgg
    24481 gcaaggtgcg ttatcatttt gttttaattt tgccagttca tcggcgtata acacagcagc
    24541 aggcggacgc tgcagatgat tattctgcgg tgacatatat tcatccctga aatgtattaa
    24601 tgaaataaac gatattttac gcgcaataaa tgtttggcgc attagttgtt tgagcgaaaa
    24661 atatctggaa agcagatcac agtaaataaa aaaacctgct ggtgaggcag gttcttttat
    24721 ttactacttt caggcctaac accacagcct gtgcaacatc gtgttccaac gaacgaatta
    24781 taaccacggc tggaccccgt tgcaattaag ataatgagat ttataatcca gaccgttggg
    24841 acgttgcgcc ggatcgaatc tatttcaagt cctggctacc ggacgggtgg tgctgcaggt
    24901 ggtgccctga tccggagtgc aacatcgtgg tccaacatac gtaaaccagt atcgacaggc
    24961 agtgtaaggg taaggtgctg gtgttgcgct ccttaa
//
LOCUS       DAZGXZ010000070        24935 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000070 DAZGXZ010000000
VERSION     DAZGXZ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.070"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..759
                     /locus_tag="ACHKJF_002966"
     CDS             <1..759
                     /locus_tag="ACHKJF_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0950289.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACHKJF_002967"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACHKJF_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP0950290.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACHKJF_002968"
     CDS             2581..3900
                     /locus_tag="ACHKJF_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP0950291.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPGWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4272..5654
                     /gene="dosC"
                     /locus_tag="ACHKJF_002969"
     CDS             4272..5654
                     /gene="dosC"
                     /locus_tag="ACHKJF_002969"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP0950292.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5679..8042
                     /gene="dosP"
                     /locus_tag="ACHKJF_002970"
                     /gene_synonym="pdeO"
     CDS             5679..8042
                     /gene="dosP"
                     /locus_tag="ACHKJF_002970"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP0950293.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDNKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPHSRVFI"
     gene            8262..8843
                     /gene="ddpX"
                     /locus_tag="ACHKJF_002971"
     CDS             8262..8843
                     /gene="ddpX"
                     /locus_tag="ACHKJF_002971"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP0950294.1"
                     /translation="MSDTTELVDLAVIFPDLESELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYNPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8857..10406
                     /locus_tag="ACHKJF_002972"
                     /pseudo
     CDS             8857..10406
                     /locus_tag="ACHKJF_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            10408..11430
                     /locus_tag="ACHKJF_002973"
     CDS             10408..11430
                     /locus_tag="ACHKJF_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0950295.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGNPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGKLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11427..12323
                     /gene="ddpC"
                     /locus_tag="ACHKJF_002974"
     CDS             11427..12323
                     /gene="ddpC"
                     /locus_tag="ACHKJF_002974"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP0950296.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12320..13306
                     /locus_tag="ACHKJF_002975"
     CDS             12320..13306
                     /locus_tag="ACHKJF_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0950297.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13299..14225
                     /locus_tag="ACHKJF_002976"
     CDS             13299..14225
                     /locus_tag="ACHKJF_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP0950298.1"
                     /translation="MSNTLLTLCDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEQTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14281..14712)
                     /gene="osmC"
                     /locus_tag="ACHKJF_002977"
     CDS             complement(14281..14712)
                     /gene="osmC"
                     /locus_tag="ACHKJF_002977"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP0950299.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            15058..15273
                     /gene="bdm"
                     /locus_tag="ACHKJF_002978"
     CDS             15058..15273
                     /gene="bdm"
                     /locus_tag="ACHKJF_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP0950300.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            15375..15512
                     /gene="sra"
                     /locus_tag="ACHKJF_002979"
     CDS             15375..15512
                     /gene="sra"
                     /locus_tag="ACHKJF_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP0950301.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15669..17366
                     /gene="maeA"
                     /locus_tag="ACHKJF_002980"
     CDS             15669..17366
                     /gene="maeA"
                     /locus_tag="ACHKJF_002980"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0950302.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            17500..18510
                     /gene="adhP"
                     /locus_tag="ACHKJF_002981"
     CDS             17500..18510
                     /gene="adhP"
                     /locus_tag="ACHKJF_002981"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP0950303.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            18656..18940
                     /locus_tag="ACHKJF_002982"
     CDS             18656..18940
                     /locus_tag="ACHKJF_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0950304.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(19168..19821)
                     /gene="fdnI"
                     /locus_tag="ACHKJF_002983"
     CDS             complement(19168..19821)
                     /gene="fdnI"
                     /locus_tag="ACHKJF_002983"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP0950305.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19814..20698)
                     /gene="fdnH"
                     /locus_tag="ACHKJF_002984"
     CDS             complement(19814..20698)
                     /gene="fdnH"
                     /locus_tag="ACHKJF_002984"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP0950306.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20711..23758)
                     /gene="fdnG"
                     /locus_tag="ACHKJF_002985"
     CDS             complement(20711..23758)
                     /gene="fdnG"
                     /locus_tag="ACHKJF_002985"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(23171..23173),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0950307.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPCCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23990..24871
                     /gene="yddG"
                     /locus_tag="ACHKJF_002986"
     CDS             23990..24871
                     /gene="yddG"
                     /locus_tag="ACHKJF_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP0950308.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAVWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         6098 a         6342 c         6313 g         6182 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggttgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc accctggtag tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc atccgcaacc cacgtcaatg aaatgagcaa cccagggcgc gactatccgt
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggt ggtttgtcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggggtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccagaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac gtctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ctggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcaaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataattca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacg gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa cgagaaatta gcgattagaa gaccggcgat actggttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcgggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tgtctcgcct cgactggcca ccggtttcct cggttaacat tagtaacccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggtataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtacta tcagggaact gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgcg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gcagaagtgg tttcccgcta tcccgtagat ggcgtgcagt ttgacgacta tttctatacg
     3301 gagtcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcgcac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggtgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccttcaaaga tagagccagg ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccattc ttactttgta acaagccggt gctttaccca ccggcttgtt accctcttga
     3961 actatcccac tactaatcat gcttactaaa gtcaaattaa ccacgcaatt aaccacactt
     4021 agttaaacag ccataaatcc cctaataaaa atgtatgaat atgaaaaaac atacgatatt
     4081 cgctgtcacc gaaaaataat tacgatgggc tatactcttc tgaagaacag tagcgtagat
     4141 taaaactgtc ctaagtcgct tatttctttt cagtatatct tcatatttca ggagaatatg
     4201 gtgcagttag tcgatctcgc acgttgcgtt ttgattccgt ctgatttccc agccttataa
     4261 gggtgtgaat tatggagatg tattttaaaa gaatgaaaga tgagtggacc ggacttgtcg
     4321 aacaggcaga tcctctcatt cgtgctaaag ccgcagaaat tgcccttgcg catgctcatt
     4381 atctgagtat cgagttttat cgaattgtcc gcatcgaccc gcatgccgaa gaattcttga
     4441 gtaatgaaca agttgagcgg cagttgaaaa gtgcgatgga acgctggatt attaacgtgc
     4501 tttctgccca ggttgacgat gtcgaaaggc taatacaaat ccagcatacc gtcgcggaag
     4561 tgcatgcccg cattggaatt ccggtagaaa ttgtcgagat ggggtttcgg gtgctgaaaa
     4621 aaatcctcta tccggtaatc ttctcttcgg attattctgc cgcagaaaaa cttcaggtct
     4681 accatttctc gattaacagt attgatatcg ctatggaagt gatgacccgc gcgtttacct
     4741 ttagtgacag tagtgcctca aaggaagatg aaaactatcg tatcttctcg ttactggaaa
     4801 acgccgaaga agaaaaagaa cggcaaatag cctcaatact ttcatgggaa atagatatta
     4861 tctataaaat cctgctggat tctgatttag gcagtagttt gcctttaagc caggctgatt
     4921 ttggcctgtg gtttaaccat aaaggtcgac attattttag cggcattgcc gaagtaggcc
     4981 atatctcccg tctgattcag gatttcgacg gtattttcaa tcaaaccatg cgtaacacca
     5041 ggaatttgaa taacagaagt ttgcgggtga agtttctatt acagataaga aataccgtat
     5101 cgcaaattat taccttgctg cgtgaattgt ttgaagaagt atcgcgccac gaagtcggta
     5161 tggatgtact gacgaaatta cttaaccgcc gtttcctgcc gactatcttc aaacgcgaaa
     5221 ttgcccatgc caaccggacc ggtacaccgc tgtcagtgct gattattgac gttgataaat
     5281 tcaaagagat caacgatacg tggggccata acactggcga tgaaattctg cgtaaagtct
     5341 ctcaggcctt ttatgacaac gtccgcagta gtgattatgt tttccgctac gggggcgatg
     5401 aatttatcat tgttttgact gaagcttctg aaaacgaaac gttacgtacc gcagaacgta
     5461 ttcgcagtcg ggtggagaaa accaaactga aagccgcaaa cggcgaggat attgccctct
     5521 cactttccat cggtgccgcc atgtttaatg gtcatcctga ctatgagcgc ctcattcaaa
     5581 tagccgatga agctctgtat atcgccaaaa gacgaggtag aaaccgtgtt gaactctgga
     5641 aagccagtct ttagatgcgc caggatgcag aggtaatcat gaagctaacc gatgcggata
     5701 atgccgccga tggcattttt ttccccgccc ttgagcaaaa tatgatgggt gcggtgttaa
     5761 ttaacgaaaa tgatgaagtg atgtttttca accccgccgc agagaagctc tgggggtaca
     5821 aacgtgaaga agtcattggc aataacattg atatgctgat tccgcgggat ttgcgtcctg
     5881 cgcatcctga atacattcgt cataaccgtg aaggcggtaa agcgcgtgtc gaggggatga
     5941 gtcgggagct gcagctggag aaaaaagacg gcagtaaaat ctggacccgt tttgcgctat
     6001 cgaaagtgag cgccgagggg aaagtttatt acctggcgct ggtacgggat gccagcgtag
     6061 aaatggcgca aaaagaacag actcgacaat tgattattgc cgttgaccat ctcgaccgac
     6121 cggtgattgt cctcgatccg gaacgccata ttgtgcagtg caatcgcgca tttaccgaaa
     6181 tgtttggtta ctgcattagc gaagccagcg gtatgcagcc cgatacactc ctgaacattc
     6241 ctgaattccc tgccgataac cgcattcgtt tacaacagtt gctatggaaa accgcccgcg
     6301 atcaggacga atttctgctg ttgacgcgca ccggtgaaaa aatctggatt aaagcctcta
     6361 tcagcccggt ttatgacgtg ctcgcgcatc tgcagaacct ggtaatgact ttctcggata
     6421 tcaccgaaga acggcaaatc cgccagcttg aaggcaatat tctcgccgcc atgtgcagca
     6481 gcccgccatt tcatgaaatg ggggaaatca tttgtcgtaa catcgaatct gttctcaacg
     6541 aatcgcatgt ttcgctgttc gcactgcgca acgggatgcc gatacactgg gcgtcatctt
     6601 cgcacggtgc agaagttcaa aatgcgcaaa gctggtcagc gaccattcgt cagcgtgatg
     6661 gcgcgcctgc ggggatcctg caaattaaaa cctcgtcagg agcagaaacc agcgccttta
     6721 tcgaacgcgt ggcagatatc agtcagcata tggccgcgct ggcgctggaa caggaaaaaa
     6781 gccgccagca tattgaacaa ctcatccaat ttgatccgat gaccggtctg ccaaatcgca
     6841 ataacctgca caattacctc gatgacctgg tcgacaaagc cgtctctccg gtggtgtatc
     6901 tcatcggtgt tgaccatatt caggatgtga ttgatagcct tggctatgcg tgggccgatc
     6961 aggcattgct ggaagtggtc aatcgctttc gtgaaaaact caaaccggat cagtatctct
     7021 gtcgtatcga aggtacgcag tttgtcctcg tgagcctgga aaacgacgtc agtaacatta
     7081 cccaaatcgc cgatgagcta cggaatgtgg tcagcaagcc gataatgatt gacaataaac
     7141 ccttcccgct taccttgagt attggcatca gctacgacgt gggtaaaaac cgcgattacc
     7201 tgctctccac tgctcacaat gcaatggatt atattcgcaa gaatggcggt aacggctggc
     7261 agttcttcag cccggcgatg aacgaaatgg taaaagagcg tttggtttta ggcgcagcgc
     7321 tgaaagaagc gattagcaat aaccaactga aacttgttta ccagccgcaa atcttcgcag
     7381 aaacgggtga actgtacggc atcgaagccc ttgcgcgctg gcacgatccc cagcatggtc
     7441 atgtgccccc ttcacggttt attcctctcg cagaagagat tggtgaaatc gaaaatattg
     7501 ggcgctgggt catcgcggaa gcttgccgtc agttagcaga atggcgtagc cagaatattc
     7561 atatcccggc gctttccgtt aacttgtcgg cactgcactt tcgcagtaat caactgccta
     7621 atcaggtgtc tgatgcaatg cacgcctggg gtattgacgg ccaccagctg acggtagaaa
     7681 tcacggaaag catgatgatg gaacacgata ccgaaatctt taagcgcatt cagatcctgc
     7741 gtgatatggg cgtgggctta tcggtagatg attttggtac gggcttttcc ggattatccc
     7801 gcttagtcag tcttccggta acggaaatca aaattgacaa aagttttgtc gatcgttgtc
     7861 tgaccgaaaa acgcatcctt gccttacttg aagccattac cagcattggg caaagcctca
     7921 atttaaccgt cgtggcggaa ggcgtcgaaa ccaaagagca atttgagatg ctacgcaaga
     7981 tccactgtcg cgttattcag ggatatttct tttctcgccc gcactcgcgg gttttcattt
     8041 aacgtgacac tgtcacttaa atactgtgat ttcagctacg actccgggag attccttcct
     8101 taacttcccc tcttgcacca aaactgcact acgctgcaca aatgttgatc aaaagttaaa
     8161 aaatataact ttaagccagc attctggcgc ttatcccggc atggcatgag atctgcataa
     8221 gcggaaagcg cagcaatttt tgtcttatac aggaaccgtt tatgtcggat accaccgaac
     8281 tggttgattt agccgtaatc ttccctgatc tggagagcga attgaaatac gcctgcgctg
     8341 ataacatcac aggtaaagct atttatcagc aagcgcgttg tctgctacac aaggatgcga
     8401 ttaccgcgct ggcgaaaagt atcagtatcg cccagctgtc aggcttacaa ctggtgattt
     8461 acgatgcgta tcgcccacaa caagcacagg cgatgttgtg gcaagcctgc ccagacccgc
     8521 aatatgttgt tgatgtgacg gtcggttcta atcacagccg tggcacggcg atcgacctga
     8581 cgcttcgtga tgagcacggg aacatcctcg atatgggcgc agggttcgat gaaatgcacg
     8641 agcgctccca tgcctataac ccttccgtcc cgcccgccgc tcagcgcaat cggctgttgc
     8701 tgaatgcgat aatgactggt ggcggttttg tcggtatctc cagcgaatgg tggcacttcg
     8761 aattacccca ggcagcgagt taccctctgc tcgctgatca attcacctgt tttatatccc
     8821 ctggcacaca acacgtcagt taatcaggaa actgtcatga agagatcgat accgtttcgt
     8881 cccacattgc tcacgctcgt ccttgccaca aatttcccgg ttgcgcacgc cgccgtaccg
     8941 aaagatatgc tggtgattgg taaggccgcc gatccacaaa ccctcgaccc ggcggtaaca
     9001 atagataata acgactggac ggtgacctac ccgtattatc agcggctggt tcagtacaaa
     9061 acggacggtg ataaaggctc acccacgttg aaggcgatct ggcaagtagc tggaaagcgt
     9121 ctgacgatca aaaagagtgg acgttcaccc tgaaggacaa tgctaaattt gccgatggca
     9181 caccggtcac tgccgaagca gtaaaactct cttttgagcg gctactaaaa atcggccagg
     9241 ggccagcaga agcatttccc aaagatttaa agattgatgc tcccgacgaa catacggtga
     9301 agtttaccct tagccagcca ttcgcaccgt tcctctacac gctggcgaat gacggtgcat
     9361 ccattatcaa tccggcggtc ttaaaggaac atgcagcgga tgatgctcgc ggcttcctcg
     9421 cgcaaaatac cgccggttcc ggaccattta tgctgaaaag ctggcaaaaa ggtcagcaat
     9481 tagttctggt gccaaatccg cattacccag gcaataaacc gaacttcaaa cgggtatcgg
     9541 taaaaattat tggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc gacattgaca
     9601 ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaat aaagtcaatg
     9661 tggcagagta tccgtcactg cgcgttacct atctgtatct gaataacagc aaagcgcctc
     9721 ttaatcaggc ggatctgcgt cgggccattt cctggtctac cgattatcag ggaatggtta
     9781 acggcattct gagtggtaac ggaaaacaga tgcgcggccc gattccggaa ggcatgtggg
     9841 gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa gccgaatggg
     9901 ataaagtgac gagcaaaccc accagcctga cgtttctcta ctcagataac gatccgaact
     9961 gggagcctat tgctctggcg acacaatcca gtctcaacaa gctgggcatc aatgtgaagc
    10021 tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat tacgacattg
    10081 cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat tactggtttg
    10141 agtcagacaa aaaaggtctg ccaggcaacc gctcgttcta tgaaaacagt gaggtcgata
    10201 agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccgg gactaccagc
    10261 aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctattccag aaaaactacc
    10321 aactagcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg gaacaggtct
    10381 tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg ccaacgctgc
    10441 tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat ctcgcatctg
    10501 atccctggca atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc tatcgtggaa
    10561 aatattcgcc agcaactggg actggaccag ccactgtatg tacagtttta ccgctacgtc
    10621 agcgacctgt ttcatggtga cctgggaaca tccattcgta ccgggcgtcc ggtgctggaa
    10681 gagttgcgta tatttttccc ggcgacgctg gaactggctt tttgtgccct gctgctggca
    10741 ctcctgattg gcatcccgct gggcatactc tctgcagtct ggcgaaatcg ctggctggat
    10801 catctggtgc gaataatggc cattaccgga atctccacac ctgcgttctg gcttggactg
    10861 ggcgtcattg tgctgtttta tggtcatctg caaatacttc ccggcggcgg aaagcttgat
    10921 gactggctgg atccaccaac gcacgttacc ggcttttatc tgctcgatgc gctgcttgaa
    10981 ggcaacggtg aagtcttctt caatgcgttg caacatctca tcttaccggc attaacgctg
    11041 gcgttcgttc acctgggaat tgtcgcccgc cagattcgct cagcgatgct ggaacaattg
    11101 agtgaagact acattcgtac cgcccgggcc agcggcttgc ccggctggta tatcgtttta
    11161 tgttatgcgc tacccaatgc gttgatccca tcgattaccg tattgggttt ggcgctgggc
    11221 gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg aatgggtgca
    11281 tgggtagtaa catcaataca ggcgctcgac ttcccggcag tgatgggctt tgccgtcgtg
    11341 gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt gtggattgat
    11401 ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc gccgtacgcc
    11461 cacaaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa ggcagtccgc
    11521 tcaccgtgac cggcgcagtc atcattgtat taatgctatt gatgatgatt ttttcaccgt
    11581 ggctggcgac gcatgatccc aatgccattg atttaaccgc ccgccttttg ccgccttctg
    11641 cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gttcagccgc gtactggtcg
    11701 gcagtcagca atcaattctc gccggattag tcgtggttgc cattgcgggt atgattggtt
    11761 cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc atcatgcgca
    11821 tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg gctgccgctc
    11881 tcgggccgag tttatttaac gccatgctgg cgattgctat tgtgcgaatt cccttttatg
    11941 tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacctatgtt caggcggcga
    12001 aaacctttgg tgcgtcgcgt tggcatctga tcagctggca tattttacgt aactccctgc
    12061 cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg gccgccacgt
    12121 tgggatttat tggcctgggt gctcaacaac cgagtgctga atggggcgcg atggtagcga
    12181 atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg gcagcgattt
    12241 tgcttaccgc cgttgggttt aatctctttg gtgatggtat tcgcgatctg cttgacccga
    12301 aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacagc tgcatttgag
    12361 tttccccggt tttaacggtg atgttcacgc gctcaacaat gtgtccttgc agattaaccg
    12421 cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca ccgcaatgct
    12481 gattatgcgt ctgctaccga cgggcagtta ttgcgtacat cgggggcaga tttcactgct
    12541 gggagacgat gttcttaacg ctcgggaaaa acagcttcgt cagtggcgcg gcgcacgagt
    12601 ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacgtcgaa taggtcttca
    12661 gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca gagctaaagc
    12721 gattgccctg ctggaagaga tgcaaatccc ggatgccgtg gaagttatgt cgcgctatcc
    12781 gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat tctcctgcga
    12841 gccgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgc tacagttgca
    12901 ggtactgcgt ttgcttaaac ataaagcccg cgccagtgga actgcggtac tgttcatcag
    12961 ccatgatatg gtcgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt atgccggaag
    13021 cgtaattgaa agcggcgtga cggcagacat tatccatcat ccccggcatc cgtataccat
    13081 tggtttgctg caatgcgcac cagaacatgg agtaccacgc cagccattac ccgccattcc
    13141 agggacggta ccaaacctca cccatttgcc tgaaggctgc gcttttcgcg atcgttgcta
    13201 tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg acaacaacca
    13261 gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt gtctaacacg ttattaacgt
    13321 tatgcgacgt ccatatcaat ttcccggccc gtaaaaactg gcttggtaaa actacggaac
    13381 atgttcatgc cattaatggt attgatttac agatccgccg tggtgaaacc ttagggatcg
    13441 tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggt atgctgcaac
    13501 cgagccacgg gcagtacatc cgttcaggat cacaacgcat tatgcagatg gtgtttcagg
    13561 acccgctctc ttcgctcaat ccgcgcttac cggtgtggcg catcatcaca gaaccgctct
    13621 ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt agcagaggag ttggctgtgc
    13681 aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc ggcgggcagc
    13741 ggcaacgcat cgccattgcc agagcactct cttcgcagcc tgacgtgatt gtgcttgatg
    13801 agcaaacctc tgcactggat atctccgtgc aggcgcagat cctcaattta ctggtaacgc
    13861 tacaggtaaa tcgcgggctg acctatgtgc tgatctcaca caatgtctcg gtgattcgtc
    13921 atatgagcga tcgggtagcg gtgatgtatc tcgggcagat tgtagaactg ggcgacgcgc
    13981 agcaggtgtt gacggcacct gcacatccat acacccgatt attgctggat tccctccccg
    14041 ccattgataa accgctggag gaagaatggg cattacgtaa aacggatctg ccgggaaacc
    14101 gcacgttgcc gcaaggctgt tttttctacg aacgttgccc tctggcaacc cacggatgtg
    14161 aagtccggca atcattaaca ataagggagg acggacgtga gatccggtgc tggcgggcgc
    14221 tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc cggcaatgct
    14281 ttacgatttc aactggtaat caagcgtaat ttccgctttc agtacctgag aaaccgggca
    14341 tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac ccggcaccgc
    14401 aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt tatccagcga
    14461 cacatcggcg gtggtatcaa tcgatgttgg cgtaaatccc gcttccccca gcattaatga
    14521 aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag ggttagttcc
    14581 tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca caccactctc
    14641 ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct gacctttctt
    14701 atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac tacgtgagaa
    14761 acagcacgag gataagcagg atattccgaa taaaaatccg gcagaaatat tacttctgca
    14821 cttaaattaa atttccacaa tagataaccg ggaatatata acccaagaga tattgcatta
    14881 atattaatca actcaccaca ataataatac ctatcaatat catgaggtta aaatataaaa
    14941 atcatcaacc aggactaatc ttaacaacga agcggcaaat atttgccatg ctgaatgtgc
    15001 tttataaatc tgcgatccgt agcagacacc ataaatacac agacacggag aatcactatg
    15061 tttacttatt atcaggcaga aaattcaaca gcagaacccg ctctggttaa tgccattgag
    15121 caaggccttc gggcagagca cggtgttgtc actgaagatg acattctcat ggagctgacc
    15181 aaatgggtgg aagcttctga taacgacatc ctcagtgata tctaccagca aacgatcaat
    15241 tatgtggtca gtggccagca ccctacgctt taaggtgcta tgcttgatcg gcaacctaat
    15301 ttaggggttt agcacgtgtt tcttcgctac ggcgatgttg tccttaaaac tagcaacagg
    15361 attgaggagt taaaatgaaa tcgaaccgtc aggcacgtca tattcttgga ctggaccata
    15421 aaatttctaa ccagcgcaaa atagttaccg aaggtgacaa atccagcgtg gtaaataacc
    15481 caaccggcag aaaacgcccc gctgaaaagt aattcataac catcagtcct caatgacgat
    15541 taaacaccat tgcctgcgca atggtgtttt tgtttttatc tgctttatac ttggggccga
    15601 cgccctggcg gtaaagcaaa gacgataaaa gccccccagg gatggatatt caaaaaagag
    15661 tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct tacgctggcc
    15721 cggtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg gaagaacgcc
    15781 gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa gaacaagcgg
    15841 aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac atctacctgc
    15901 gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat catcttgatg
    15961 agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt ttttctgaga
    16021 tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccgccacaat atggacgata
    16081 ttctgcaaaa cgtaccaaac cataatatca aagtgattgt ggtgactgac ggtgaacgta
    16141 ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt aaactgtcgc
    16201 tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg ctggatgtcg
    16261 gaacgaacaa ccaacagttg cttaacgatc cgctgtatat gggctggcgt aatccgcgta
    16321 tcactgacga cgaatactat gaattcgttg atgaatttat ccaggctgtg aaacaacgct
    16381 ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg ccgttactta
    16441 accgctatcg caatgaaatt tgttccttta acgatgacat tcagggcact gcggcggtaa
    16501 cagtcggcac cctgatcgca gcaagccgcg cggcaggtgg tcagttaagc gagaaaaaaa
    16561 tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc atcgcccaga
    16621 cccagcgcga aggattaagc gaggaagcgg cgcggcagaa agtctttatg gtcgatcgct
    16681 ttggcttgct gaccgacaag atgccgaacc tgctgccttt ccagaccaaa ctggtgcaga
    16741 agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gctgtcactg ctggatgtgg
    16801 tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg ctgtttacgg
    16861 aagagatcat ccgtgagatg cataaacact gtccgcgtcc gatcgtgatg ccgctgtcca
    16921 acccgacgtc acgcgtggaa gccacaccgc aggacattat cgcctggact gaaggtaacg
    16981 cgctggtcgc cactggcagt ccgtttaatc cagtggtatg gaaagataaa atctacccta
    17041 tcgcccagtg taacaacgcc tttattttcc caggcatcgg gctgggtgtt attgcttccg
    17101 gcgcgtcacg tatcaccgat gagatgctga tgtcggcaag tgaaacgctt gctcagtatt
    17161 cgccgctggt cctgaacggc gaaggtctgg tactaccgga actgaaagat attcagaaag
    17221 tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc gtggcggtga
    17281 aaacctctgc cgaagccctg caacaggcca ttgacgataa tttctggcaa gccgaatacc
    17341 gcgactaccg ccgtacctcc atctaagcct gcgcccggta gtgaaggcta ccgggctatt
    17401 tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat ctcatttgcc
    17461 tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaaggctgc agttgttacg
    17521 aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca tggcgaagcc
    17581 ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa gaatggcgat
    17641 tttggtgaca aaaccggcgt aattctgggc catgaaggca tcggtgtggt ggcagaagtt
    17701 ggcccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg gttttacgaa
    17761 ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg ttcagttaaa
    17821 aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt cgccgattac
    17881 gcggtaaaag tgccagatgg tctggactcg gcggcggcca gcagcattac ctgtgcggga
    17941 gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg gattgctatc
    18001 tacggtcttg gcggtctggg taacctcgcc ctgcaatacg cgaagaatgt ctttaacgcg
    18061 aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga aatgggtgca
    18121 gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt
    18181 ggcgctcacg ctgcggtggt aacagcagta gctaaagctg cgtttaactc ggcagttgat
    18241 gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc tatgagcctg
    18301 gatatcccac gtcttgtgct ggatggtatt gaagtggtcg gttcgctggt cggcacgcgc
    18361 caggatttaa ctgaagcctt ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc
    18421 ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg caaaatccgt
    18481 ggccgtatgg tgattgattt ccgtcactaa gaggcctttg ctgcgactgc catgttcggg
    18541 tcgcagcatc gcacactctc caacatgaaa tggctaaatg aattattgtt gcgtaagaag
    18601 gaacaataat gatactgttc ccagtgaagg aacactactg agagtaaaga cactgatgaa
    18661 aatggccaat catccccgcc cgggggacat tattcaggaa tcactggacg aacttaatgt
    18721 cagcctgcgc gagtttgcca gagcaatgga aattgctccc tcaacggcaa gccgattgct
    18781 gaccggaaaa gcagctttga cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag
    18841 ttcgccgcaa atgtggctga atctgcaaaa cgcctggagt ctggcagagg ctgaaaaaac
    18901 ggtggatgtg tcgcgattac gccgtctggt tacgcaataa aaaatggcac cttataaagc
    18961 gccatttgaa actgatgaca aacgcaacat tgcctgatgc gctacgctta tcaggcctac
    19021 acgttcactg caatctattg tatttgaatg gttttcatgc cggataaggc attcacgacg
    19081 catccgggat gaacaaagcc cacgttgtct acaatatgaa aatgacgcct tttacagcgc
    19141 cattttcagc ttaaagttca aaagagatta tatcccttct tcactctctt ttttcgcttc
    19201 tgccttctcg atttcacgat accagcgcgg atggtgtttc ttcgcccagc gacggctcac
    19261 cttcccttcg atcatccctt taatcgatcc tttcacccag aatgccatat acatatggat
    19321 caggatggcg tggatcagga tgatacccgc agccgcgtgg atcagcaggc tataacggac
    19381 aacctgcatc gggaagtact gcgcaaagta cggacgccag ataatcaccc cggtcaccag
    19441 cagcacgaaa atcatgctca tgattgacca gaacatcatc ttttgcccgg cgttgtactt
    19501 accgacatcc gccactttat gctcattgcc tttcagtact tcgacaatgt tcaacagcca
    19561 cggaatatct ttcttatccg ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag
    19621 cgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaata cgtcccatct gcggcgtacc
    19681 gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac
    19741 caggaagaag caaatcacca cggtccagtg acaggcgcga tcaataaatt tggtgcgcac
    19801 aatcattttc gacttactca tgatgatcct cctcgtcatc gtccacttcc ttattcgggc
    19861 caataccgat gtagtggaaa atcaacccgg caaaagtggc aataaagcca gccgctgcca
    19921 gcggtttcaa cgcgcctttc cacaggctta ccgaggtgtc gatcttcgga tctttcggca
    19981 gaccgtgata cagctccggc tgatcggcgt gatgcagcac gtacataacg tgcgtaccac
    20041 cgaccccttc cgggttgtag acgccagcat gttcgtaacc acgcgctttc agtttcgcca
    20101 cgcgctgttc cgccagctcc agcatctcct tcttggtgcc gaagtggata gccccggtcg
    20161 gacaggtttt cacacaagcc ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc
    20221 atttatatac ccggttatcc tctttgttga ggcgcggaat attaaacgga cacccggcaa
    20281 tgcagtaacc acagccgatg cagttttccg actggaaatc gacaatcccg ttagcgtact
    20341 gaatgattgc accagcagac gggcacgcct taaggcagcc cggatcttca cagtgcatac
    20401 agccgtcttt acggatcagc cactccagct tgccgttctg ttcggtttcg ctaaagcgca
    20461 tcaccgtcca ggacttggcg ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc
    20521 ccacttcatc acggatgtcg ttccactccg aacacgccac ctgacaggct ttacagccga
    20581 tacaggtgga aacgtcgata agtttcgcga cttctgcttt gtaatcacgc acctgagaag
    20641 gcggcgtgat ggagttagtt gcggaccttt tgataatgtc ctgcgtttcc atagccatct
    20701 gttcgccccc ttacgccttc tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg
    20761 agtttgcatc accgacattc ggcgtcagag tgttagcgat ataaccttta cgcgcgacac
    20821 cctcaaagcc ccagtggatt ggaataccca ccgtttcaac ctgctgaccg tttacattca
    20881 gcggtttcag acggcgcgtt accaccgcca ccgcgcggat aaagccacgc ttgcttgaga
    20941 cagtgacacg atcgccatta ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca
    21001 caaactgttc cggctgagca attgcgttga gcaatgcgtg cttggtccag gtgtggaagt
    21061 gctcggtcag acgataggtc gtacccacat acgggaactg ctctttttta cccatccgca
    21121 gtgcgtcttg ttcatacaga cgaacaaccg ggttagacac cacgttcgga tgcagcgggt
    21181 tagtgcccag cggcgtttca atcggctcgt agtgttccgg gaacggacct tccgccattt
    21241 tgttgatggc aaacaggcgt cccatccctt ccggctgcat gataaacggc ccggttggcg
    21301 tacccggtgc ggcattgccg aagtcaggaa tatcgttacc cgtccacttg ctgccgttcc
    21361 actggatcag catccgtttc ggatcccacg gtttaccgtt gatatccgcc gaagcacggt
    21421 tgtacagcac gcgacggttg agcggccacg cccaggccca tcccagcgta ttccccagac
    21481 cggacgggtc ggagttatcg cggttagcca tctggttgcc ctgctctgtc cagctaccgg
    21541 tgtagatcca gcaagaagat gcggttgtac cgtcatcacg cagatgcgca aagctactca
    21601 gcaactgacc tttcttcgca atcagcacgc cattagcatc atagagatct tccagcgcat
    21661 agccgttgtt ctctttagct acttcgtcag attgcggttc gtgcggctgc ttgtagttcc
    21721 agctcatctt catcagcggt tctacgcctt taccaccttc ggcctggtac agctcgcgca
    21781 ggtgatggta gatacccgcc agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct
    21841 gacctttcca gtgccactgc aaccagcgac cggagttagc gatagaacca tcttcttcgg
    21901 caaagcaggt cgaaggcaga cggaatactt cagtctgaat agacgccgga tcgacatcgt
    21961 tcgactcgcc gtggttctgc cagaaggtag aagtttcagt caccagcgga tcgataacca
    22021 ccatgtactt cagcttgctc aggcagctca ccactttgtt tttgtccggg aaggacgcaa
    22081 ccgggttaaa gccctggcag aaataaccgg tgactttgcc ttcatccatc atgttgaaat
    22141 acttgatgac gtcgtaggtc tggtcccact tcggcagcca gtcatagccc cagttgttct
    22201 ctttctgcgc ggcatcgcca tagaaagatt tcatcaggct aacgaagaac ttcggatagt
    22261 tgctccagta gttcacctga tcagccagcg tcgctttcgg cgtgttcgct tccagatacg
    22321 actgcaaatc aacctgtttt tctgacggca gcgtcagata acctggcagg ctggtagaga
    22381 gcaggcctaa gtcagtcaga ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc
    22441 caccggccat acccatgtta ccgagcagca actggatcat cgccatagta cggatgttct
    22501 gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg
    22561 gtgcgctggt ggaggccagc acttcacaca ctttcaggaa gtcggctttt ggcgtaccac
    22621 agatgttttc aacaacgtcc ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca
    22681 cacagcacgg atgagtcagt gtttcatcgc gtttcgcata gccgttttca tcgaactgat
    22741 agttccagga cgatttatcg tattgccgtt tttcagcgtc gtagccgctg aacagaccgt
    22801 cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt ggtgtaatgt ttaacgtatt
    22861 cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt
    22921 ccgtaccgga acgaataggt gcgtaaatat ccgccacaga agcggtacgc gtaaaacggg
    22981 gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac
    23041 cgacgggatg cgcttcagca gcgttaccgc ccattaccat tacgacgtta gcgtttttga
    23101 tatccaccca gtggttggtc atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg
    23161 ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag catcccgagg gagcgggcaa
    23221 atttctgtgt cagcatcccg gtttcgttgc tggcaccgga ggcacacagc ataccggtag
    23281 aaagccaacg gtttaccgtt acgccctgct cgttcttttc aataaagtta gcgtcacggt
    23341 cagctttcat cagctttgca atacgggaga atgcttcttc ccagctaatg cgctgccatt
    23401 tgtcagaacc tggcgcacga tattccgggt agcgcagacg gttttcgctg ttgacgtaat
    23461 ccagcaaacc ggcccctttt gggcacagcg caccacggct taccggatga tccgggtcac
    23521 cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc atcacccagg ctatacatca
    23581 atagcccgca acctacggaa cagtatgtgc aggtgttacg gatctcttta gcgcgtaata
    23641 atttgtagtt tcgcgcctga gccagtgctt gcttcggggc aaagcccaat gccgctaccg
    23701 ttgttccagc cataccgccc gcgcagattt taaaaaattg tctgcgactg acgtccattg
    23761 ctttcctctt ttttcagggg tattactgcg cgaggaaact aacagtaaaa ttctgaatct
    23821 ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa taatccgcct gcgcaaattg
    23881 acctacctca atagcggtag aaaaacgcac cactgcctga caggccagtt aaaaaaatgc
    23941 tataaaattc agcttaattt ttaacggcaa gagagacaaa acagcgagca tgacacgaca
    24001 aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg agcacgatgg taggattgat
    24061 tcgcggtgtc agcgaggggc tcggcccggt tggcggcgca gctgctatct attcattaag
    24121 cgggctgctg ttaatcttca cggttggatt tccgcgtatt agacaaatcc cgaaaggcta
    24181 tttactcgcc gggagtctgt tattcgtcag ctatgaaatc tgtctggcgc tttccttagg
    24241 gtatgcggcg acccgtcatc aggcgattga agtgggtatg gtgaactatc tatggcccag
    24301 cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa accaactggc tgattgtacc
    24361 tggattatta ttagcccttg tcggcgtctg ttgggtgtta ggcggtgaca atgggttaca
    24421 ttatgatgaa atcatcaata atatcaccac cagcccattg agttatttcc tggcgtttat
    24481 tggtgcgttt atctgggcag cctattgcac agtaacgaat aaatacgcac gcggatttaa
    24541 cggaattacc gtttttgtcc tgctaacggg agcaagtctg tgggtttact attttcttac
    24601 gccacaacca gaaatggtat ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt
    24661 taccttagga tttgcttatg ctgtatggaa tgtcggtata ttgcatggca atgtcaccat
    24721 aatggcggta ggctcgtatt ttacgcctgt actttcctca gcgcttgcag ccgtgctgct
    24781 cagcgccccg ctgtcgttct cgttctggca aggcgcgcta atggtctgcg gcggttccct
    24841 gctctgctgg ctggcgacac gtcgtggtta aaataattgc cgggtctacg gcccggcagt
    24901 taatcagata aattccgaca gtattaaaaa ataaa
//
LOCUS       DAZGXZ010000071        24600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000071 DAZGXZ010000000
VERSION     DAZGXZ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.071"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(83..1087)
                     /gene="argF"
                     /locus_tag="ACHKJF_002987"
     CDS             complement(83..1087)
                     /gene="argF"
                     /locus_tag="ACHKJF_002987"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP0950309.1"
                     /translation="MSRFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1249..1665
                     /gene="rraB"
                     /locus_tag="ACHKJF_002988"
     CDS             1249..1665
                     /gene="rraB"
                     /locus_tag="ACHKJF_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP0950310.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1711..2214)
                     /gene="yjgM"
                     /locus_tag="ACHKJF_002989"
     CDS             complement(1711..2214)
                     /gene="yjgM"
                     /locus_tag="ACHKJF_002989"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0950311.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2407..3603
                     /gene="yjgN"
                     /locus_tag="ACHKJF_002990"
     CDS             2407..3603
                     /gene="yjgN"
                     /locus_tag="ACHKJF_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP0950312.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3659..6514)
                     /gene="valS"
                     /locus_tag="ACHKJF_002991"
     CDS             complement(3659..6514)
                     /gene="valS"
                     /locus_tag="ACHKJF_002991"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP0950313.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6514..6957)
                     /gene="holC"
                     /locus_tag="ACHKJF_002992"
     CDS             complement(6514..6957)
                     /gene="holC"
                     /locus_tag="ACHKJF_002992"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP0950314.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7091..8602)
                     /gene="pepA"
                     /locus_tag="ACHKJF_002993"
     CDS             complement(7091..8602)
                     /gene="pepA"
                     /locus_tag="ACHKJF_002993"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP0950315.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8756..8806
                     /gene="ytgA"
                     /locus_tag="ACHKJF_002994"
     CDS             8756..8806
                     /gene="ytgA"
                     /locus_tag="ACHKJF_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP0950316.1"
                     /translation="MSIIILATISTTQENK"
     gene            8869..9969
                     /gene="lptF"
                     /locus_tag="ACHKJF_002995"
     CDS             8869..9969
                     /gene="lptF"
                     /locus_tag="ACHKJF_002995"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0950317.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            9969..11051
                     /gene="lptG"
                     /locus_tag="ACHKJF_002996"
     CDS             9969..11051
                     /gene="lptG"
                     /locus_tag="ACHKJF_002996"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0950318.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11170..12672)
                     /gene="yjgR"
                     /locus_tag="ACHKJF_002997"
     CDS             complement(11170..12672)
                     /gene="yjgR"
                     /locus_tag="ACHKJF_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP0950319.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAIIQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMISELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12750..13748)
                     /gene="idnR"
                     /locus_tag="ACHKJF_002998"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(12750..13748)
                     /gene="idnR"
                     /locus_tag="ACHKJF_002998"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFP0950320.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYQGNTL"
     gene            complement(13815..15134)
                     /gene="idnT"
                     /locus_tag="ACHKJF_002999"
     CDS             complement(13815..15134)
                     /gene="idnT"
                     /locus_tag="ACHKJF_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFP0950321.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSSVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(15199..15963)
                     /gene="idnO"
                     /locus_tag="ACHKJF_003000"
     CDS             complement(15199..15963)
                     /gene="idnO"
                     /locus_tag="ACHKJF_003000"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP0950322.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(15987..17018)
                     /gene="idnD"
                     /locus_tag="ACHKJF_003001"
     CDS             complement(15987..17018)
                     /gene="idnD"
                     /locus_tag="ACHKJF_003001"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFP0950323.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            17235..17798
                     /gene="idnK"
                     /locus_tag="ACHKJF_003002"
     CDS             17235..17798
                     /gene="idnK"
                     /locus_tag="ACHKJF_003002"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0950324.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(17802..18821)
                     /gene="ahr"
                     /locus_tag="ACHKJF_003003"
     CDS             complement(17802..18821)
                     /gene="ahr"
                     /locus_tag="ACHKJF_003003"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP0950325.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            19017..19101
                     /locus_tag="ACHKJF_003004"
     tRNA            19017..19101
                     /locus_tag="ACHKJF_003004"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19051..19053,aa:Leu,seq:caa)
     gene            19190..21137
                     /locus_tag="ACHKJF_003005"
                     /pseudo
     CDS             19190..21137
                     /locus_tag="ACHKJF_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313269.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
     gene            complement(21476..22651)
                     /locus_tag="ACHKJF_003006"
     CDS             complement(21476..22651)
                     /locus_tag="ACHKJF_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012137237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0950326.1"
                     /translation="MQTNPEKCIFCDIPFDKGSLEHVFPSALGGRITTTHATCKSCNN
                     LFSEASSDAVEIALADNFIYIRNALNVWSGRGNPPPTIKEAGQFDDGIKYDLAPNLTP
                     IVSKSRIPSKDETDSNTVFDFVAQDVGDANRIVGILKKRGLNIGDINAKYVTTKAPVI
                     RASIKFEGNKIFRAIAKIAVVSYVVLYGNSRARTDIYQSLRGSIRSGEPDITKYCGWD
                     YTNDFPVITNLHPHEKTSDAIQCGFEHTVFITNVNHQLVAYIKLFGAFNFSIILGNHS
                     SISPKCLCLNPTAGKSSRFNVLFNPPLSYIPKNIDSFKIEHESVRKHVQLAMSSIVEH
                     CQSLSTEEYIRSLSQELMISVQTASVDSDISEIIRSFSEKLAHIENGLAWEEEINIE"
     gene            complement(22852..23184)
                     /locus_tag="ACHKJF_003007"
     CDS             complement(22852..23184)
                     /locus_tag="ACHKJF_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HFP0950327.1"
                     /translation="MRTVEILQYTLRKGSGAAFHAIMQEISVPLHQSNGIDVVSFGNS
                     LHDLDCYYLIRAFDSAESMTAVLNAFYASADWRSGPREDIVGSIENCIKTVISLPSES
                     VKGLRVQS"
BASE COUNT         6092 a         6054 c         5966 g         6488 t
ORIGIN      
        1 ggaataattg aactattact gcataaaata atgtgatgcc gggatgtttt tgtatttccc
       61 ggcatcttta tagcgatatc aattatttac tgagcgtcgc gaccatcacc gctttgatgg
      121 tatgcatgcg gttttccgcc tgatcaaaaa caatgctggc ggcagattcg aagacctcat
      181 cagtcacttc cattccgcca tgtaggccaa actcttccgc cattttctta ccaagcgtcg
      241 tctggtcgtc atgaaacgcg ggcaggcaat ggaggaattt gacctccggg ttaccggtca
      301 actgcatcat tttgctgttc acctgataat cacgcagtaa tgcaatccgt tctgcccatt
      361 tctcttttgc ttcccccatc gacacccata catcggtata gataaagtca gcaccttcaa
      421 ctcctttggc tacatcttcc gtcagcgtaa tattcccgcc attttgctgt gccagggcgc
      481 ggcattccgt aaccagcgca gcttccggcc agcatgcctg tggcgcgacc agacgcaaat
      541 ccagaccggt aagcgccgca gcttcgagca tcgaattgcc catgttgtta cgtgcgtcac
      601 ctgcatagac cagcgtcatt tcgttgaatg ctttaccggg taaatgctcc tgcatggtga
      661 ggagatccgc cagcaactgc gtggggtgaa actcattggt caggccgttc cataccggca
      721 cgccagcgta ttccgccagt gtttcgacaa tctcctgacc atagccgcga tactgaatac
      781 cgtcatacat gcgacccagc acgcgggcag tgtctttaat cgactcttta tgaccaatct
      841 gactgccgct tgggccgaga taagtaacgc gagcaccctg gtcatatgcg gcaacttcga
      901 aagagcatcg ggtacgggtc gagtcttttt cgaagatgag cgcgatgttt ttaccagtga
      961 gtttggcttc ttctttgccg cttttcttat cggctttcag tttcgcggct aactgcagca
     1021 ggctgttgag ttcagctggc gtgaaatcga gtaatttcag gaaatgctta tgataaaacc
     1081 tggacataga tccctcctgt ggctaacgcc tcaatgaatt aaaattcaat ttatatggat
     1141 gattattcat ttgcaagtct aaagcataaa tctttgtcac aaaggtggag gcaatgtcag
     1201 tggtgtgtga caataagagt atcggcagga cattaagagg aatgagccat ggcaaacccg
     1261 gaacaactgg aagaacagcg tgaagaaaca cgtttgatta ttgaagaatt actggaagat
     1321 ggcagcgatc cggacgcgct gtacaccatc gaacaccatc tttccgcaga cgatctggaa
     1381 accctggaaa aagcagcagt tgaagcgttt aaactcggtt acgaagtgac cgatccagaa
     1441 gagctggaag ttgaagatgg tgatatcgtg atttgctgcg acatcctcag cgagtgcgcg
     1501 ttgaatgccg atctgatcga tgcccaggtt gaacaactga tgacgctggc ggagaaattt
     1561 gacgttgagt acgacggttg gggcacttac tttgaagatc ctaacggcga agacggcgac
     1621 gatgaagatt ttgtcgatga agacgatgac ggggttcgcc actaattaat tgattaaaat
     1681 gcccgatagc acacactagc gggcattttt ttagagttcg cgtagcatcc gcacttcaca
     1741 atctacatgg cccgtgcagc caagtgcata gtcgatatgt tgaaaaccta aatgttcata
     1801 caaaccaatc gcttctttca aaaaagcggt cgtttccaga tagcagcgtt tgaagcccat
     1861 ctctcgtgcc tgctccatcg ccattaaggc cagttttttc gccagccctt taccgcgaat
     1921 agccggaaga aaatacatct tctgcagttc gcaaatgtcc gactcactcc cggttaatgg
     1981 cgcaatccca ccgccgccga ccacttcacc atcgtactca accacccaat atgcatgacc
     2041 cggctgacta tacacttgat acaactcgtc caggttcgga tcggcgacgg tgtagccttt
     2101 atcagcggta agaccgtatt cggcggatac ctgacgaatg acgcgggcga tggcgggatt
     2161 atcctgctgc gtcaggcgac gcattacagg tgattgcggc gcaatgttat tcatagttta
     2221 acccaataaa aataaagtaa ttatacattt gtttaatacc actctcttct tacgtgcgca
     2281 agcgacttat aaagtaataa agtttttgcc agcctattac tgataagagt attgtaacgt
     2341 ggcagaacat tttgctgtct gtatatataa attatatcaa tggatttttt aattcaggga
     2401 atttttatgg ctcaagttat taatgaaatg gatgttccgt cccattcgtt tgtttttcat
     2461 ggtacaggtg agagatattt tcttatttgt gtggtgaatg tgttgttaac gattataacg
     2521 ctaggtatct atttgccatg ggcattaatg aaatgtaagc gttatctcta tgctaatatg
     2581 gaagttaacg gacaacgatt ttcttatgga attactggtg ggaatgtttt tgttagttgt
     2641 cttgtttttg ttttttgcta tttcgcaatc ttaatgacag tgtcagcaga tatgccactt
     2701 gttggctgtg ttttgacttt gttactgttg gttttgctta tatttatggc agcaaaagga
     2761 ctgcgttatc aggccttgat gaccagtctc aacggcgtaa gatttagttt taattgctct
     2821 atgaaagggt tctggtgggt aacctttttc ttgccgattt taatggccat tgggatgggg
     2881 actgttttct ttatctcgac aaagatgcta catgccaata gttcaagtag tgttattata
     2941 tctgtggttc tgatggcaat agttggtatt gtttccattg gtatttttaa tggtacttta
     3001 tatagcctgg taatgagttt tctctggagc aataccagtt tcggtataca tcgtttcaag
     3061 gtgaaattag atactacgta ttgtataaaa tatgccattc tcgcattttt agctttatta
     3121 ccttttctcg ctgttgctgg ttatattatc ttcgatcaaa tattaaatgc gtatgatagt
     3181 tctgtgtatg caaatgatga tattgagaat ttacagcaat ttatggaaat gcaacgtaaa
     3241 atgataatcg cgcagttaat ctattatttt gggattgctg ttagcaccag ttatttaacg
     3301 gtgtcgttgc gaaatcattt tatgagcaac ctgtcactga atgatgggcg tattcgtttt
     3361 cgctcaactt taacgtacca cggtatgctt tatcgcatgt gtgcgttggt ggtgatatcc
     3421 gggattacgg gcggtctggc ttatccactg ctgaaaatat ggatgattga ctggcaggca
     3481 aaaaatacgt atttgctggg cgatttggat gaccttcctt taatcaataa agaagaacaa
     3541 ccagataaag gcttcttagc caggatttca cggggaatta tgccttcttt accatttctg
     3601 taagagataa aaaaggccgg agcatgctcc ggccttcgtt ttcattactg tgttttgatt
     3661 acagcgcggc aataacagcc tgctgttcaa tcagcttcgc tttcgcttct gcatagcctt
     3721 ccagcttctc acgctctttc gcgatgaccg cttccggtgc gcgggcgaca aagccttcat
     3781 tcgccagctt gttctcgata cggctgattt cgccttcgat cttcgccact tctttcgcca
     3841 gacgcgccag ctcatcttct ttgttgatga ggccagccat cgggatcagc agctctgcgc
     3901 cgtcgatgat cttagtaacg gaaaccggac ctttgtcatc ggcaggcagc acggtgatgc
     3961 tttccagacg cgccagggtt tgcaggaagc cacggttttc atttacgcga cgttctgcat
     4021 ccgcgctgca accacgcagt agcagctcca gcggtttgcc cggcgcgatg ttcatttctg
     4081 cacggatgtt acgtaccgca acgatcgcct gcttcagcca ctcggtgtcg gccagtgcgg
     4141 cttcatcaac ctgagatgca tcgtactgcg ggaacggctg cagcatgatg gtgtcggcag
     4201 tgataccgca aagtactttc acacgctgcc agatggtttc ggtgatgaac ggaatgatcg
     4261 gatgcgcgag gcgcagcaga ccttccagta cagtcaccag cgtatggcga gtaccgcgca
     4321 gttctgcttc ggtgccaccg ttcattaccg gcttggtcag ctcgagatac cagtcacaga
     4381 actggttcca ggtgaactca tacagaatgc ctgcggcgat atcgaagcgg aagctgtcca
     4441 gcgcttcgcg gtacgctttg atggtctggt tgaactccgc cagaatccag cggtccgcca
     4501 gcgacagcgt catttcgccg ccgttgaagc cgcaatcctg accttctgtg ttcatcagca
     4561 caaagcggct ggcgttccac agcttgttac agaagttacg gtaaccttcc aggcgcttca
     4621 tatcccagtt gatgtcacga ccggtagacg ccagtgccgc cagggtgaag cgcagcgcgt
     4681 cagtaccgtg cggttcaata ccgttcggga actgcttctc ggtgcgctta cggattttgt
     4741 ccgccagctg tggctgcatc atattgccgg tgcgtttttc cagcagttct ggcagcgaaa
     4801 taccgtcaac catatccagt gggtcgataa cgttaccctt ggatttggac atcttctggc
     4861 cttcgtcgtc acgaatcaga ccggtcatgt aaacagtgtg gaacggcacc tgcggtttgc
     4921 cattttcatc tttgatgaag tgcatggtca tcatgatcat gcgggcaatc cagaagaaga
     4981 tgatgtcgaa accagatacc atcacgctgg ttgggtggaa ctgacgcagg gcgtcggtgt
     5041 tttccggcca gccaagggta gagaaggtcc acagcgcaga agagaaccag gtgtcgagaa
     5101 cgtcttcgtc ctgacgcagg acaacatcag caccgaggtt attttcttta cgcacttcgt
     5161 cttcgttgcg gccaacataa acgttacccg cttcgtcata ccatgccggg atacggtgac
     5221 cccaccacaa ctgacgagag atacaccagt cctgaatatc gcgcatccag gagaagtaca
     5281 tgttttcgta ctgcttcggt acgaactgaa tgtcgccgtt ctcaaccgct tcaactgccg
     5341 gtttcgccag gacatcggca cgcacgtacc actggtcggt cagcatcggt tcgatgacca
     5401 cgccaccacg gtcgccataa ggaacggtca ggtcatgtgg tttaatttct tccagcaggc
     5461 cgagtgcgtc aaccgcagca acgaccgctt tacgtgcagc aaaacgctcc agtttctgga
     5521 attctgcggg gatctcgctg gaataaacgt cagattcgtt acctttggta tcgaacacct
     5581 gggcactttc acggatatcg ccgtcaaagg tcaggatgtt gatcatcggc agcgcgtgac
     5641 gtttacccac ttcatagtcg ttaaagtcgt gcgccggagt gattttcacg cagccggtgc
     5701 ctttttccat gtcggcgtgt tcgtcgccaa cgatcggaat acgacggtta accagcggca
     5761 gaatgacata tttgccaatc agatctttgt aacgcggatc ttccgggtta acggctacgc
     5821 cagtatcgcc cagcagggtt tccggacggg tagtcgcaac caccagataa tctttaccgt
     5881 ctgcggtttt cgcgccgtca gccagcggat agcggatgtg ccacatcgaa cctttcgatt
     5941 cgcggttttc cacttccagg tcagagatag cggtgcgcag tttcgggtcc cagtttacca
     6001 ggcgcttgcc acggtaaatc aggtcttctt tatacagacg aacgaaaact tctttcaccg
     6061 cattggacag gccttcgtcc atggtgaagc gttcacgctc ccagtccacg gagttgccga
     6121 gacggcgcat ctgacgggta atggtgccgc cggattccgc tttccattcc cagattttgt
     6181 cgatgaaagc ttcgcggccg tagtcgtgac gggttttacc ttcttctgcg gcaatcttgc
     6241 gctcaacgac catctgggta gcgatcccgg cgtggtcagt accgacctgc cacagggtgt
     6301 ttttgccctg catgcgctga tagcggatca tggtatccat gatggtttgc tggaaggcgt
     6361 gacccatatg caaactgccg gtgacgttcg gcggcgggat catgatgcag aaactttcct
     6421 ggctttcatc gccattaggc ttaaagtagc cctgcttttc ccagtgctcg taaagcggct
     6481 gttcgatatc ttgtgggtta tatgtctttt ccattatttc caggttgccg tattcaggtt
     6541 gaaaccagcc acgcggtagg ctttatagcg ttcgcgcgcc agttgtttca gagaatcttc
     6601 ataaggaacg aagtctacca cttctgtgaa agcggtggca aaatctgcaa agcttgttcg
     6661 cagactaatc aatatatcgc gcgggctgct gctgcgtttt tgcggccagg cgatctccac
     6721 cggcgcaccg ccgcgcggtc cttctcccgc taaattatgc ggaacaaagc tttctgccgg
     6781 acgcgcccac agggcttcat ccagccggta agcctgcttt tcatcttcac aggcgatgag
     6841 cacgcgctta ccgctgcgcc aacgttctgc ggcaatttca cacaccagtt gctcaacggc
     6901 gcttaagcca tcgacggtgg tgtcattgtc cagaaggtag aacgtcgcgt ttttcataaa
     6961 tggggcttct tgtggtggat ttaaatattg ctgcattcct caccctaaaa gatgagcaat
     7021 gaatttctat tgcgctcgat cggttccctc tcccttaggg agagggttag ggtgagggta
     7081 aaacgcaaac ttactcttcg ccgttaaacc cagcgcggtt taacaggaac tgtgccagca
     7141 acgctaccgg acgaccggtg gcgccttttg ctttaccaga acgccaggcg gtaccggcga
     7201 tatccaggtg cgcccagttg tacttacggg taaagcgtga caggaagcaa cctgcggtaa
     7261 tcgccccacc aggacgaccg ccaatgttcg ccatatcggc aaaattggac tccagttgtt
     7321 cctgatactc gtcacccagc ggtaagcgcc atgcgcggtc accggattgt tcagacgcgg
     7381 caatcagttc atgggccagc ggattatggt tcgccatcag accagtaata tgatgaccca
     7441 gcgcgatcac gcaggcaccg gtcagcgtcg ccacgtcaat caccgcttcc ggctcaaaac
     7501 gctcaacgta agttaacacg tcgcacagta ccaggcggcc ttcagcgtcg gtgttcagca
     7561 cttcaacggt ttgaccggac atggtggtta acacatcgcc cggacgatag gctcgtccgc
     7621 caggcatgtt ttcgcagcct gccaacacgc cgataacgtt aatcggcagt tgtagctccg
     7681 cgaccatccg catcacgccg taaaccgctg ccgcaccgca catatcgtac ttcatctcat
     7741 ccatgccttc tgaaggcttg atcgagatac cgccggagtc gaaggttaaa cctttaccca
     7801 ccagcacgat tgggcgtgca tcttccgacg cgttgccttt gtactcaatc accgacatca
     7861 gcgattcgtt ttgcgaaccc tgaccgaccg ccagatagga atgcatcccc agctctttca
     7921 tctgctgttc gccgataacg cgggtgatga cattcttgct gtagctgtca gccagctggc
     7981 gcgcttgtga agcgaggtaa gcggcgttac agatattcgg cggcatattg ccgagatctt
     8041 ttgctgcttt aatcccggcg gcaatcgcca gaccgtgctg gatcgcgcgc tcaccgctgg
     8101 tcagttcacg gcgggtcggc acgttgaaca ccattttacg cagcggacga cgcggttcgc
     8161 tcttgttcgt tttcagctga tcgaaactgt agagcgtctc ttttgctgtc tcgacagcct
     8221 gacgcacttt ccagtagttg ttacggcctt taacgtgcag ttcagtcaga aagcagaccg
     8281 cttccattga gccagtatca ttcagcgtat taatggtttt ctgaataacc tgcttgtact
     8341 gacgctcatc cagctcacgt tctttgccgc aaccaataag gagaattcgc tcggaaagta
     8401 cattcggaac atggtgcagc aacaatgtct gccccggttt tccttccagt tcgccccgac
     8461 gtagcagggc gctgatgtac ccatcgctga ttttatcgag ctgttctgca atcggagaaa
     8521 ggcgacgtgg ttcgaagacg cccacgacga tgcaggcact ccgctgtttc tccgggctac
     8581 cgctttttac actaaactcc atgcactacg ctcctgaatc ttaaagacaa cggcggtggc
     8641 tacagataga attgcaagct ttcgtaactc atgtccgctg ttgcgatgac ttcgtgttaa
     8701 tcttaacgtt attacggcat tggcacgtca gaacaaaatc tgagaggtga atccgttgag
     8761 tataattatc ttagcgacga tttcgacgac tcaagagaat aaatgacgtt taagccatga
     8821 aacaagctaa aatcctgcaa aagacgagtt tttacgggcg tatttaaagt gataatcata
     8881 agatatctgg tgcgggagac gctcaaaagc cagctggcga tactcttcat cttgcttttg
     8941 atcttcttct gtcaaaagtt agtgaggatc ctcggcgcag cggttgacgg cgatattccg
     9001 gcgaatctgg tgctctccct tctcgggttg ggcgtgccgg aaatggcgca gcttatcctg
     9061 ccattaagcc tgttcctcgg gctgctgatg acgctgggca aactgtatac cgaaagtgaa
     9121 attacggtaa tgcatgcctg cggcctgagc aaagcggttc tggtgaaagc ggcaatgatc
     9181 cttgcggtat tcacggcaat cgtcgcggcg gttaacgtga tgtgggcggg accgtggtca
     9241 tcgcgtcatc aggatgaagt gttagcagaa gcgaaagcga accctggcat ggcggcgctg
     9301 gcgcaagggc aattccagca agcgactaat ggcagctcgg tgctgttcat cgagagcgtt
     9361 gacggcagcg atttcaaaga tgtgttcctc gcgcaaattc gaccaaaagg taatgcacgt
     9421 ccttctgtgg tggtggccga ttccggacat ttaacccagc tgcgcgacgg ctcccaggtc
     9481 gtcactctca accagggaac gcgcttcgaa ggcactgcac tgttacgtga tttccgcatt
     9541 acggatttcc aggattatca ggcgatcatt ggtcaccagg cggtggcgct cgacccgaac
     9601 gataccgacc agatggacat gcgcacattg tggaacactg acaccgatcg tgctcgcgca
     9661 gaactgaact ggcgtatcac gttggtgttc accgtgttta tgatggcact tatggtcgta
     9721 ccgctgagcg tggttaaccc gcgtcagggg cgcgtactgt cgatgctgcc agccatgctg
     9781 ctgtatctac ttttcttcct gatccagacc tccctgaaat cgaatggcgg taaaggtaag
     9841 ctagacccga cgctgtggat gtggaccgtt aacctgattt atctggcttt ggcgattgtt
     9901 ctcaaccttt gggacaccgt gccggtccgc cgcctgcgcg ccagtttttc gcgtaaagga
     9961 gcggtgtgat gcaacctttt ggcgtacttg accgctatat cggtaaaact attttcacca
    10021 ccatcatgat gacgctgttc atgctggtgt cgctgtcggg cattatcaag tttgtcgatc
    10081 agctgaaaaa agccgggcag gggagttacg acgcgttagg cgcaggaatg tataccttgc
    10141 tgagcgtgcc gaaagatgtg caaatcttct tcccgatggc ggctctgctt ggggcgttgc
    10201 ttggtcttgg gatgctggcg cagcgcagcg aactggtggt gatgcaggct tctggtttta
    10261 cccgtatgca ggtggcgctg tcggtgatga aaaccgccat tccgctggtc ttgctgacga
    10321 tggcgattgg cgaatgggtc gcgccgcagg gcgagcagat ggcgcgtaac taccgtgcgc
    10381 aggcgatgta cggcggctcg ttgctctcta cccagcaagg cttatgggcg aaagatggca
    10441 acaacttcgt ctacattgag cgggttaaag gtgacgaaga gttaggtggc atcagcattt
    10501 atgcctttaa cgagaatcgt cgtctgcaat ccgtacgcta tgccgctact gcgaagtttg
    10561 acccggaaca taaagtctgg cgtctgtcgc aggttgatga atctgatctg accaatccga
    10621 aacagattac cggttcgcag acggtgagcg gcacctggaa aaccaacctc acgccagaca
    10681 aactgggcgt ggtggcgctg gacccggatg cactctcaat cagcggtttg cacaactacg
    10741 tgaagtatct gaagtcgagc ggtcaggacg ccggacgtta tcagctcaac atgtggagca
    10801 aaatcttcca gccgctatcc gtggcggtga tgatgctgat ggcgctgtcg ttcatctttg
    10861 gcccactgcg tagcgtaccg atgggcgtgc gtgtggtcac cggtatcagc ttcggttttg
    10921 tcttctacgt actggaccag atcttcggcc cgttgacgtt ggtttatggc atcccgccga
    10981 tcatcggcgc actgttgcca agcgccagct tcttcttaat cagcctgtgg ctgttaatga
    11041 gaaaatcgta accaagcaag ggagcgaaag ctccctttca cattgttgat aaagtcaaat
    11101 acgagttcga tcggtcccct cgcccctttg gggagagggt tagggtgagg gggacagatc
    11161 gcgcctgact taccttcttc tcccccccag caaactcccc aacattccac gcacaatctg
    11221 attcgtcacc tggcgagcgg cgcttttggc cattgtttgc accacaccat ctttcttccc
    11281 gccgcgtggt ccggtagtgc cgaacaaaat atccttcaat ccaccaagaa tgccgtcatc
    11341 caccgctacc tctttccctt tcgcgggggg attattttgc tgctcggtac tggcctgaaa
    11401 gcctttttgc aacatctcat aggcggattc ccggtccacc tcatcctcat atttgccata
    11461 caccggagag tgattaatca agccattacg ctcatcttcc gtcaccggcc ccatccgcga
    11521 acaaggcgcg atcaccatcg cacgctccac cacagaaggg cttcctttcg catccagaaa
    11581 agagatcaac gcctcgccgg tgcccagttc ctgaatcgcc ttttcggtat caaacgccgg
    11641 attggcccgc atggtttgcg ccgcagcttt taccgctttc tgatctttgg gcgtaaaagc
    11701 ccgcaaagcg tgctgaacgc gattaccgag ctggccgagc acattatccg gaatatcaga
    11761 cgggttttgc gaaacgaacc agacgcccac gccttttgag cgaataagcc ttatcacctg
    11821 ctcaatctta tccagcagta cctgcggtgc atcgttaaac agcagatgtg cttcgtcgaa
    11881 gaaaaacacc agttttggct tctccagatc gcctgcttcc ggcaattgtt catacaactc
    11941 tgaaatcatc cacagcaggc tggcggcgta cagtttcggc atctgataaa gtttctcggc
    12001 gctgaggata ttgataacgc ctttaccgtt ggcatcggtg cgcatccagt ctttgatatc
    12061 cagcatcggc tcaccaaaga agtgtgctgc gccttgctgt tccagcgaca acagcccgcg
    12121 ctggatggca ccaaccgatg cgctgctgat atttccgtac tgattctgga aagatttggc
    12181 gttatcgccg atgtactgga taatcgcccg caaatcttta aagtcgagca gtaacagccc
    12241 ctgatcgtca gcaatgcgga agatgatatt cagcacgcca gattgcacat cgttgagatt
    12301 caacagccgc gccagcaaca gcggccccag atccgaaacc gtcgcccgca ccggatggcc
    12361 cttctcgcca aagatatccc acaccaccac cggattggca tgcggttgcc agtcgttgac
    12421 accgatattt ttcaaccgag cgagcagttt ttccgatgcc gttcctgcct gcgcgatgcc
    12481 ggtcagatcg cctttcacat cggccataaa caccggaaca ccgatttccg atagagactc
    12541 cgccagtttt tgcagcgtga cagttttacc cgtccccgtt gcgccagtaa tcagcccgtg
    12601 acggttagcc attcccggca gtaaaaacag ttctgtgtcc ggcgtgcggg caattaacag
    12661 gggttcgctc atgagatatc ctccagtttc ctgcctggag tataggcaac gtccggcaaa
    12721 gagaatacgt gtaatgaaga gaaaataacc taaagcgtgt tgccttgata aatctgatac
    12781 ccgaggtcaa cagtgttgtg gttgtgatcg ttatttttaa ttttgctcag caacatttgg
    12841 gcggccatcc gaccaatatc aaaacgggga gtaattacgc tggcgaggct tgggatcatt
    12901 tgcctgccaa tctccagccc atgaaaaccc gcaatggaaa tttgttctgg cacggcgagg
    12961 tttcgctcgc ggcacagcag aagcgcaccc atcgcaatat cgtcattggt gcaaaataca
    13021 ccgtctaaat ccgggtttgc gctaagggca tcacgcatta actgcattcc taaatgaatg
    13081 gatgagatgg cgcgtggatt catgcgtaat ggggaaagat tatgcagcat catcgcatcg
    13141 caatacccct gataacgctg ttcatcgcgg gtgtcatctt tggaaccgag atacagaatt
    13201 ttgtgcctga ctcgcttctc cagcatggta cacaccatgt caaaagcggc ctgccgatta
    13261 tcaaaaccga cctccatatc cagccgttct ccctgtacat ccatcaattc cacgaccgga
    13321 atggtggctg aacgcagaaa tttcaccgtc ctgatggtgt gatatttttc cgaaagtata
    13381 atcccgtcaa tgttataaga gagcaggtta atcaccgact cttcttcgga atcgcgatcg
    13441 taattgtaat tagcaataag ggtctgataa ttatgctcag aggtgacgga ttctattccc
    13501 gcgagaatgt cagcgaagag ttgattttga aatgacggaa tcaagatacc aagggtataa
    13561 ctttgtgcat tcaacaacat gccgggagcg cgattaggga tataattgat ctcttccata
    13621 attttggcga tgcgctcgcc tgtttccttt gccacctttt tcggcgagcg gatataacga
    13681 ctcacggtca tttttgttac gccagccagc gtagcgatat cctgtaaaga aattctgtga
    13741 ttgcgcataa cttctcccaa cgtcggggga atcttcaatc ttcccccgaa actacatcac
    13801 aatattttat tctttcagtg caatacggcg ttaatggcta acacgcccag caaacccata
    13861 atagaaataa gggtttccat caccgtccag gtacgcaacg tttcaccaac cgtcagatta
    13921 aaataccctt taaatagcca gaagccaggg tcgtttacgt gtgacgcgat cacgctgccc
    13981 gcaccggttg ccagtaccat taaagcggga tcggcatggg taacgttgat aatcggcaac
    14041 accacacccg cggtggtaat ggccgcgacg gtggcagagc ccagagcgat acgcaacagc
    14101 gccgcaacag tccagcacat caataacggc gaaagcgtag ttccggtcat taagtgtgaa
    14161 atatagtgcc cgacactgct atctactaat acctgcttaa acgcgccgcc gccagcgata
    14221 ataaacacaa tcatcgcgat agcgcctata gaatccccaa tgatatccat gatttgctcg
    14281 atggtgcgtc cattacgtcg gcccagtgtg aaaatcgcaa taacaatggc aataaacagc
    14341 gcaacggcag ggttaccgac aaattcaaag aagaggcgca cggtgttagt tttcggtaac
    14401 gtaatttcac aaacggcggc gatagccatc aggatgaccg ggatcacggc agcgaaaata
    14461 ctgttccaga aggagggcat ctcctcttcg ctaaacagat gaggattaaa taagccttcc
    14521 ggtggtgctt tctcaaagcg agttagcagt ttagaaaaca gcggtcctgc gacaataact
    14581 gtcggaatgg taatgataaa tccatacagt aaagtcgttc cgagattagc ctcaaagata
    14641 gtcgcgatgg cagtaggccc tggatgtggc ggcagaaaac agtgggttac agagagcgct
    14701 gctaccattg gtacgccaac atacaacagg ggtaatcctg atgatgctac gatggtaaat
    14761 accaacggca acagcaggac aaaacccact tcaaaaaaca tggcgaggcc cacaaccaga
    14821 ccggtgatca ctagcgccca ttgcacgcgt tttttaccaa aagtagcaat cagcgtagtg
    14881 gcgatacgtt gtgccgcacc cgtatcagaa atcagcttgc ctaacatggc cccgaaaccg
    14941 agaatcattg ccagcccgcc gagcgtgctg ccgataccat tttgtataga gtgcaggacg
    15001 gcctgtgcat ccatcccttc ggcaaatccg acgacggcag ctaccagaac gagggcaata
    15061 aagccgttaa ctttaaagcc gatcatcagg ataagaagca gcgcgacgcc tgccgcaata
    15121 atgattaatg gcatagtctt tatctcttat tagtgccaga aaagcagcgc tttcgcgctg
    15181 cacgtagggt agtaggtgtt aaacagccac taacatgccg ccatcaacaa acaacaggtg
    15241 gccgtttacg aaatcagagg cttttgaaga aaggaacacc gcagcaccaa tcagttcctg
    15301 cggatctccc cagcgtgcgg cgggggtccg tttgcacaac caggcggtga aggcttcgtc
    15361 ctcaaccagt gctttagtca tttctgtttt gaaatagccc ggcgcaatac cgttgacctg
    15421 aatattgtgg cgcgccagct cgacgcacat gccgcgggtg agcattttta ccgccccttt
    15481 cgatgcggca taaggggtga tggtgtcacg tcccagttcg ctttgcatcg agcaaatatt
    15541 aataacttta cctgccttgc gttcaaccat gtgacgagtt accgcttgcg ataccaggaa
    15601 cacggcggtc tggtttactg cgatcacatc attccactct tgttcaggga actcagtaaa
    15661 aggatgacgg cgctggatac cggcgttatt caccagcaca tcaatggggc cgatgtcctt
    15721 ttcgatatgt tcaacggcgg catcaatttc atgtttatga gtaacattaa aaggtgcggc
    15781 aacggcctga ataccctcct ggtggagttt ttctacagca agttctgcgc gttcggcagt
    15841 aatatcatta ataattattt gtgcgccata tttacccagg ccggttgcca gtaaaaagcc
    15901 aatgccctgt gctgaaccgg taatcaagat attttttcct gccagtgaaa atagatcgtt
    15961 cattgtatta ttcctttatt tatctcttag aaaacaagct ggacttttgc tgcctgggtt
    16021 ttatcaccgg cgaaacgtag cgcctcttcc aggtcagtga agggatattc agcactcagt
    16081 aaaggcagtg gattgataac gccattcgcc agccatgaca ctgcggtatt aaattcgctg
    16141 gtaaaacgga aagagcctct gagtgaaatc tccttaccaa tcaacgtcat cattgggaat
    16201 tctgccatcg cgcctcccat acctacctgc accattacgc cgcgtgcacg agtgacctcc
    16261 agacaggtat tcactgatga aggatgaccg gacacttcaa agctgacatc gaaatagcct
    16321 ttttccgctt tccagtgatc catgtcgtcg ttttgtgggt ttacgagcac atccgccccc
    16381 atctctttgc ccagcgaaag ggaacgggga ctcacatcag cacagacaat ttccgcggcc
    16441 cccagtgttt tcactgcact gacaatcagg cagccaatgg gtccaacacc ggaaataaat
    16501 actcgcttgc cctgtaactc gccggcctga tgtgcggcat gaatcgcgac ggctaaaggt
    16561 tcggcaaaag ccataacctt ttcgtcagct ttggccggat aagggacaca ttgcgacgtt
    16621 tcgaccattt tataacgggt aaaaccacca tcaacatgag ggaaatacat ggcactgcca
    16681 aaaaaacgca tctctgtaca ctgattctcg ttatgttcaa tgcagtattt gcagtgaccg
    16741 cacggtttag acggattaat ggctaccgtt tgcccttcat gtaattctga tgagtcgcta
    16801 tgaataactt taccgataac ttcatgacct aacaccatcg gtgcctttat catgaaatta
    16861 cctacttttc cttcctgata ataatgtaaa tcggaaccgc aaattccacc tcgggttatt
    16921 tgtactaatg ttccattatt attccaatct atcgtctgct cggtaacggc aacagttttc
    16981 ttgcccgcaa caacgcagga ctgtgttttc acttgcataa attcctcact ggtcaggtag
    17041 acacctcgga agcatttaag cggttttaat tgtcattatt tgtgatgaag atcacgtcag
    17101 aaaattgtta cattactatg ttacgcataa cgtgatgtgc cttgtaattc ttatcagcag
    17161 aaataaaaaa acgtgaaatt attatgccac caggcgtagt atcgcagcag gtaagatgat
    17221 tcaggagatt ttaaatggcg ggtgaaagct ttattttgat gggcgtttca gggagtggta
    17281 aaacattaat tggtagcaag gttgccgcgt tattatctgc taaatttatt gatggtgacg
    17341 atcttcatcc agccaaaaat atagataaaa tgtcgcaggg tattccatta tctgatgaag
    17401 atcgacttcc ctggctggaa cgcttaaatg atgcttcata cagtctttat aaaaagaatg
    17461 aaacaggatt tattgtctgt tcatcattaa aaaaacatta tcgtgatatt ttacgcaagg
    17521 gtagccccca tgttcatttc ctctggttag atggcgacta tgaaactatc ctcgcgcgaa
    17581 tgcagcgtcg ggctgggcat tttatgccgg tagcgttact aaaaagtcag tttgaggcgc
    17641 tggagcgtcc acaagcagat gaacaggata ttgtgcgcat tgatatcaac catgatattg
    17701 ccaatgtcac cgaacaatgt cggcaggctg tgctggcgat acgacaaaac agaatatgtg
    17761 cgaaagaggg cagcgcctca gatcagcgct gcgaatgatt ttcaaaaatc ggctttcaac
    17821 accacgcggt aacgcgcctt accgtcgcgc acatgctgga tggcgtcgtt aattttcgac
    17881 atcgggaaca gttcggttgt cggcgcaacc ttgctgcggg cggcaaagcg catcagctta
    17941 cgcagttcat aaggcgtgcc ggtagcagag ccagagacgc tgcgatcgcc cgcaattaac
    18001 gtaaaggccg gaacagacag cggcgtgaga accgcaccga cagtgtggaa attaccgccg
    18061 tacgtcagcg cctcaaaata aggctgccag tcgaggctga cgttcacggt attgataatg
    18121 agatcaaact gccccgccag tgctttcagt gcctgcggat cgcggctatt caccacttta
    18181 tcggcaccca tcgccagcaa ttcctgctct ttcgccggat tagaactaaa ggccgtcacc
    18241 tcacatccca ttgcgtgcag aagttttata gcgatatgcc ccagcccgcc aataccaatt
    18301 accccaacgc ggctggtagc agtgatatgg tgcatcaaca gtggtttaaa gaccgtgata
    18361 ccgccgcaca acagcggccc ggcagattca atgtcgatat tttccggcag tggaataacc
    18421 cattgccagt ctacacgcaa cttctcggca aaacctccgc gattcataat tgttggcacc
    18481 gcaccttgct cacagttgat ctgatttccg ctaatacagg cgtcgcagtg accacagcta
    18541 cgcgctgtcc agccaatccc gacacgctga ccgacctgca aacctttatc ctgcgcggca
    18601 ctcccgagcg ccaccacgcg accaatcacc tcatgcccgg caaccagcgg atattgtgaa
    18661 aatccccatt cgttatcgat catcgacaga tcggaatggc agatcccgca gtaatccacc
    18721 tgcacttcaa catcttgtgg cttcagctca ccgggatcgt actcataaac ttccagttcg
    18781 ccgcccgctt cttttgcggc atagcttttt atcattgaca ttttttggtc cttctctggt
    18841 gtggtctggg aagtgtagag catggcaggg cggggtgcat ggagtgtgac aaaggttaca
    18901 catcgctgta cgcaatgctg aaaatttcag cacttagcga gatgcgagca agctggcgct
    18961 tgcatggtgg cgtgcgacag gtataatcca caacgttttc cgcatacctc ttcagtgccg
    19021 aagtggcgaa atcggtagac gcagttgatt caaaatcaac cgtagaaata cgtgccggtt
    19081 cgagtccggc cttcggcacc aagactcaat taaatccttc ttttttaagg attttttgtt
    19141 tctagacctt aatatcgcgt tgccctacta caaaagggta tacgaggcga tgtcccagcg
    19201 ttataacctc tatcgtcgaa ccagcggcat ttatgttgtc cgcattagcg ttccccaacg
    19261 ttttcgtcga tacgcaggac agtgtgagat tcacacttca acaggcactc atgatcttca
    19321 tgaagcaaag cagaagtccg cgctcctgtt ggctgtctgg tatcagacct tacaagagta
    19381 tgaacaattg gattaccgaa ctttaagtga ctgcgccccg ctgcttgctg gtgaggggat
    19441 gatctcgctt tctaactttg ctcagtcaat cgagttgcct atatcgcaat tgattcgaga
    19501 ggtgattaat cgtaacctcc cggtattctg gctggcgact ggtcagttcg gtttctatgt
    19561 tgatgaattt aatgcagtag agcgggaacc cggtgcaaaa cgagaaaaac agtctgatga
    19621 tgaaaaggat caacctaaag aagtcatcat tctcaatagc gcgtttgagc tgggtatcga
    19681 gagcttcgca aatggttatc tccgcccctt caatccccgg catactttag attgtctgtt
    19741 gagcgctgga gtatccgaag gagaggctgc atttcgaact agtggtgata accaaagtgg
    19801 aggttggttc ttcgacttac ccggcgtaga tataactgct gatagcctct tgattagcaa
    19861 agttcatgct gaaggccttc gacttacatg gctggttaag accacgccac cagcagttag
    19921 cattcaccct gccgtgcctc ttgtcgcccc tgttatcgct aacgaatatg ttcaccgcaa
    19981 acattacaat gaaaacttgt catggcttcg tgaagagtat ttgaaacatc ggcgtaaggg
    20041 caaggtatca gaagcggcgc tccgcgatat tcgctattac ttcgatttga tgatcgaagt
    20101 gatgggggat attcagttgg aagatttcga ccgtgatttc ctccgggctt atgagagcaa
    20161 gttgcgcaca attcctgcta accgtaattt gatgaaaggt aagcacgggg ttaagacgct
    20221 ggatgagtta atcgccaaag cggcagaatg tggcgataaa ctgatgacag aagagtctgt
    20281 caaaaagtat atcaacggcc tttatggtgc aatggagtgg gctgttgatg acggtaagtt
    20341 tctgaaatcg ccatgcgaca actttttccc tcccgatgac aaaggtgagc gagagcagga
    20401 tcacactgac atatttgaac cgcatgaaat taaggcaatt ttttcgcaac cgtggtttgt
    20461 ggctggaact gttgaacgta atgcgcaagg gcgattccat caatattgcc cgtttcacta
    20521 ttgggcgccg ttgttgggct tgatgacggg ggcaagggtt aacgagattg cacagttaat
    20581 gctggacgat gttctcagat gacggcgttt attacctgaa ccttgaaagc gatagcgaaa
    20641 acggaaagaa actaaaaaac gcccaatccc gccgcaagat tccggttcat tctacgctga
    20701 ttgaactcgg ttttatcgag tatgtggatg cgttgaaagc tgccgggtat gaccgtcttt
    20761 ttcccgagct taaaccacat aaaactaaag gctatggtag gccggtttcc gcatggttca
    20821 atgaatcatt gcttgcgggt cgattaaaac ttgaaagaga cagaagcaaa tctttccact
    20881 ctttccggca ttctgtttca actttgctta aagagaaggg tgttagttcg gaactgcgtg
    20941 ggcagctact tgggcatgtg cgcggcaaaa cagaaactga agtgcgatac agcaaagatt
    21001 taaaaccggt tcacatggtt gaggttgtcg aaaagattga tttttctttg cccgagatag
    21061 cgagattcaa cattcctgat gggctggatg ctgtaagtga tgcgctgcga agaaagcgtg
    21121 gcaaacaaac aggttgagtc agttactatt cagttgagtg atgctcctga atgcgctggg
    21181 acgcttccta gcgcattttt tattaaagat cgtggattta atagtgccaa atttggagac
    21241 gcttaaaatg cgtctggtga gacttgattt gcattagctc agaattgatc tgacagtttt
    21301 ctctgacagc atacaatcaa gtctatcagt ccgccttgag cgaggagcgg aaactaagcg
    21361 cttcgatagg gccgtagcat gttatgaagg agaaagctgc tttacagaaa gaatctatca
    21421 agcattcttt ctcagcaaca ataactgaat aaattttact tcgaaggagc caatattatt
    21481 caatattaat ttcttcttcc cacgccaatc cattttctat atgagcaagc ttctctgaaa
    21541 atgatctgat tatttcagat atgtcagagt caacggatgc agtttgaaca gaaatcatta
    21601 actcttggct gaggcttcta atatattctt cggtcgataa actttgacag tgctctacta
    21661 tagaactcat tgctaactga acatgtttcc taacggattc atgttcaatt ttgaatgagt
    21721 caatattttt aggtatgtaa cttaatggcg ggttaaataa aacgttaaac cttgaggatt
    21781 ttcctgctgt aggatttaag cacaagcatt taggtgatat actcgaatga ttacctaaaa
    21841 taatagagaa attaaatgca ccaaaaagct ttatataagc aaccaattgg tgatttacat
    21901 tagttataaa tacagtgtgt tcaaaaccac attgaatggc gtctgaggtt ttttcgtgtg
    21961 ggtgtaaatt tgtaatgaca ggaaaatcat ttgtatagtc ccatccgcaa tattttgtaa
    22021 tgtcaggttc tccacttcgt atggatcccc taaggctctg atagatatcg gttcttgctc
    22081 gcgaattgcc atacaaaaca acataggaga ctacagcaat tttcgctata gcacgaaaaa
    22141 tcttatttcc ttcaaactta atgctagctc ttataacagg agccttagtt gtaacatatt
    22201 ttgcattaat atcccctata ttaaggccgc gcttcttaag aattcctaca atcctattag
    22261 catcacctac atcttgagca acgaaatcaa agactgtatt gctatcagtt tcatcttttg
    22321 agggtattct tgacttagac acaataggtg tcaagtttgg tgcgagatca tacttgatgc
    22381 catcatcaaa ctgacctgct tcctttattg ttggtggtgg gtttcctctt ccagaccaaa
    22441 cattcaaagc attcctgata taaataaagt tatcagccag agcgatctca accgcatctg
    22501 aactcgcttc tgaaaataaa ttgttgcaac ttttgcatgt cgcatgtgtt gtggtaattc
    22561 ttccacctaa cgcactaggg aatacatgct ctaggctacc tttatcaaag ggaatatcac
    22621 aaaaaataca cttctctggg tttgtttgca tgtttattta acttttctat taaaataaga
    22681 atgttttaac ttaccatcat tgaactaatt tgcatcattt tttgtcgtta atcctagcaa
    22741 cacaacttga tacatgaagg accgttcttg gcacaaagct gcctgtcaga ttaggtttgg
    22801 ctatctgcct tagctgtttt aggtcaaatc taagctaata cacttgatta cctacgattg
    22861 cacacgcagc cctttcacgc tttctgatgg taggctaatc actgttttaa tgcagttctc
    22921 tatgctgcca acgatgtctt cgcgtgggcc gctgcgccag tctgcgcttg cgtaaaaagc
    22981 attgagaacc gcagtcatgc tttcagcgct gtcaaaggcg cgaatcaagt agtagcaatc
    23041 caggtcatga agcgagttgc caaacgaaac aacatcgatc ccgttgcttt ggtgtagggg
    23101 aacactgatt tcctgcatta tggcgtgaaa agcggcaccg ctgcctttgc gtaaggtgta
    23161 ttgcagtatt tcgacggttc tcaagcgtta tctccgtgtt gcccttgggc ggctcctagt
    23221 tgatagcaat aaagtaaacc ggactgggcc atgaagctat tgctaatcag acagtcatcc
    23281 gtaagcagaa gctgcgatat gcggttcaaa cgcgctgtaa ggctctctgg cgagttttta
    23341 taatggggtg taagtttcac tgttcaaaag ttgcggcgct ctctctgctt gaccgcttga
    23401 gctggcatta aacttttgca tactgccacc tgctgaatat gtggcatcgt gctgaacaat
    23461 atcaaccaga tgagtatgga tgttaatggc gacctaccga caaattgctg atgatgttaa
    23521 atcccgaacg gggaaagcag ttaaaacatg ctggatcgct catgttaaat cattgcatgg
    23581 gctgacccgt cgcgtatcac cgaaccggat cagcaccgaa agccgagttt acccttgtcc
    23641 tgaaaaatgg gtagcggaga tcgagcaatc aatgagaaat ctgggtgagc tttagcttga
    23701 ccgcgttgct taactttgga gactgtttgc ctctataagg ataagcctgc cacatccgaa
    23761 gtttttgaac gcaatgcctg ataggagggc tggcaaacct aggacatcca cttttcgacg
    23821 cgaccaattt ttgcggtagc ttttgtcgta ttggttgggg atttgtggga gctaagctga
    23881 aacttaaaaa ccacaagaac atggttcttg tgttgtcttt ctttttaact tcttgttttg
    23941 ttttatatgt tttataaacc tcttttctgg ggagcaaaac tagccctaag gaccaatgcg
    24001 atgatttgca gtgaggccct tggctaatca ggcttccaga gttgtaacct cttttctggg
    24061 tccctgcttg gatcggcgat gtgagctact ctgctttaaa aaaggctcac gacgtaagcc
    24121 ctttgaaaaa catgataact cgctattctt attggctgtg caagtccaag actcagcacc
    24181 ttacacaacc tcacgcaccc tggcatacgc cgcctttcgg aaccagacca gtgggtaacg
    24241 gtgcgcccgc tttaaacccc ggctccgcta gcggcagagc cttgttgata cgccattttg
    24301 ggtaagcgtc tgtataccaa aaccccagaa tgtatactga tagtaacatt ttgaggcttt
    24361 aattcaagtt gctgtcattg aagactggga gtaaaaagta atctaggtga agtaggttac
    24421 attaacctta tgaaaccaat agagttcatt aaaataatac ctcaggagga agaagttatg
    24481 tcgctaaaga ttattcgtgt tagtgatgct ggcgatgatc aaagtgaacg gctagtcatg
    24541 aaagtgactg atgagtgtaa tttgaaagac tttatggtaa tggattgcac ttatgaaaat
//
LOCUS       DAZGXZ010000072        24263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000072 DAZGXZ010000000
VERSION     DAZGXZ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.072"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(170..1024)
                     /gene="rclR"
                     /locus_tag="ACHKJF_003008"
     CDS             complement(170..1024)
                     /gene="rclR"
                     /locus_tag="ACHKJF_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP0950328.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            1250..2575
                     /gene="rclA"
                     /locus_tag="ACHKJF_003009"
     CDS             1250..2575
                     /gene="rclA"
                     /locus_tag="ACHKJF_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP0950329.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            2684..2920
                     /gene="rclB"
                     /locus_tag="ACHKJF_003010"
     CDS             2684..2920
                     /gene="rclB"
                     /locus_tag="ACHKJF_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP0950330.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            2932..3525
                     /gene="rclC"
                     /locus_tag="ACHKJF_003011"
     CDS             2932..3525
                     /gene="rclC"
                     /locus_tag="ACHKJF_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP0950331.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(3685..4554)
                     /locus_tag="ACHKJF_003012"
     CDS             complement(3685..4554)
                     /locus_tag="ACHKJF_003012"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0950332.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            4803..5660
                     /locus_tag="ACHKJF_003013"
     CDS             4803..5660
                     /locus_tag="ACHKJF_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0950333.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(5781..10034)
                     /gene="fdeC"
                     /locus_tag="ACHKJF_003014"
     CDS             complement(5781..10034)
                     /gene="fdeC"
                     /locus_tag="ACHKJF_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP0950334.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKLGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            10587..10676
                     /locus_tag="ACHKJF_003015"
                     /pseudo
     CDS             10587..10676
                     /locus_tag="ACHKJF_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11150..11251
                     /gene="ykgR"
                     /locus_tag="ACHKJF_003016"
     CDS             11150..11251
                     /gene="ykgR"
                     /locus_tag="ACHKJF_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP0950335.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFFHFY"
     gene            11615..11878
                     /gene="ykgM"
                     /locus_tag="ACHKJF_003017"
     CDS             11615..11878
                     /gene="ykgM"
                     /locus_tag="ACHKJF_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0950336.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            11878..12018
                     /gene="ykgO"
                     /locus_tag="ACHKJF_003018"
     CDS             11878..12018
                     /gene="ykgO"
                     /locus_tag="ACHKJF_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP0950337.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(12053..12278)
                     /gene="ykgL"
                     /locus_tag="ACHKJF_003019"
                     /pseudo
     CDS             complement(12053..12278)
                     /gene="ykgL"
                     /locus_tag="ACHKJF_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            13102..13644
                     /gene="ecpR"
                     /locus_tag="ACHKJF_003020"
                     /gene_synonym="matA"
     CDS             13102..13644
                     /gene="ecpR"
                     /locus_tag="ACHKJF_003020"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP0950338.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            13719..14306
                     /gene="ecpA"
                     /locus_tag="ACHKJF_003021"
                     /gene_synonym="matB"
     CDS             13719..14306
                     /gene="ecpA"
                     /locus_tag="ACHKJF_003021"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP0950339.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            14367..15035
                     /gene="ecpB"
                     /locus_tag="ACHKJF_003022"
                     /gene_synonym="yagY"
     CDS             14367..15035
                     /gene="ecpB"
                     /locus_tag="ACHKJF_003022"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP0950340.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            15061..17586
                     /gene="ecpC"
                     /locus_tag="ACHKJF_003023"
                     /gene_synonym="yagX"
     CDS             15061..17586
                     /gene="ecpC"
                     /locus_tag="ACHKJF_003023"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP0950341.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDHGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            17576..19219
                     /gene="ecpD"
                     /locus_tag="ACHKJF_003024"
     CDS             17576..19219
                     /gene="ecpD"
                     /locus_tag="ACHKJF_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP0950342.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            19188..19898
                     /gene="ecpE"
                     /locus_tag="ACHKJF_003025"
     CDS             19188..19898
                     /gene="ecpE"
                     /locus_tag="ACHKJF_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP0950343.1"
                     /translation="MFRRRGVTLTKALLTAVCMLATPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPECDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            20211..20540
                     /gene="ykgJ"
                     /locus_tag="ACHKJF_003026"
     CDS             20211..20540
                     /gene="ykgJ"
                     /locus_tag="ACHKJF_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0950344.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(20788..21402)
                     /gene="yagU"
                     /locus_tag="ACHKJF_003027"
     CDS             complement(20788..21402)
                     /gene="yagU"
                     /locus_tag="ACHKJF_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP0950345.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            21820..22509
                     /gene="paoA"
                     /locus_tag="ACHKJF_003028"
     CDS             21820..22509
                     /gene="paoA"
                     /locus_tag="ACHKJF_003028"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP0950346.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLFGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEIITIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            22506..23462
                     /gene="paoB"
                     /locus_tag="ACHKJF_003029"
     CDS             22506..23462
                     /gene="paoB"
                     /locus_tag="ACHKJF_003029"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP0950347.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIHPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            23459..>24263
                     /gene="paoC"
                     /locus_tag="ACHKJF_003030"
     CDS             23459..>24263
                     /gene="paoC"
                     /locus_tag="ACHKJF_003030"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP0950348.1"
                     /translation="MKFDKPAGENTIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKV"
BASE COUNT         6389 a         6072 c         5696 g         6106 t
ORIGIN      
        1 gttttattta attgaagcaa tttagtttgc atttttattt aaaaactgag ctagagtcca
       61 tcagaaaaaa atatataaag cccaaaccac cgttggaaat agaaatagtt gaaaggctca
      121 caactcccgg aatgcggatt atctttgccc tgatttctgg cggttttatt cagggtgcaa
      181 gctgtctgac cctttcccga tattctcccg gagtacaacc aaactcgcgg acaaacgcct
      241 tgtgaaaaga tgattcactg gcatagccta ctgactcagc gatcaccaca acagggagca
      301 tttcccggga aaacatctgg gccgctattt gtagacgcaa ctttgttaat acagccagcg
      361 gcgtggttcc ggaaacatca cggaaaagct gggcaaaact tgcccgggac atgtgagcga
      421 tgctggccag cgattcgacg gtccaggcgt gtcctggcat ttccagcatt tgctgtatta
      481 ccgcaccaag acgtggatgc agaagcaaac tgagaatgtt tttctcagta ttaacctgtg
      541 caatccactc acgcaccgcg agggtaaaga atgtagcgca gatctggcta cacagtgcat
      601 ccactcccgg catcgccgat ctggattcct gttgtaaaaa cgggatcgcc tccctcagcc
      661 agttatattc cacgctgtgg ttaaccggtg ctaaaaacag cgtttccggc aaagacgtta
      721 aaaaataacg cgccgaatgt tgcaaccgaa gagtgccgca gacaatacag gtcgattcat
      781 tatcgacatg actcaggcga tgagctgaat tttgtggtag caggaccaca tttcccgggc
      841 gtaatgtaaa aatctctccc gtcggcattt ccagcttcgc cgctccttgc gttaacgcat
      901 gccaacgaat aaccgataat tccccggcac catgtggaag ctgccagtca cttcctaaca
      961 cgcaattctt atcgatcgtt ccttgtggag cgttaagcat caacaaacgg ctaagggcat
     1021 ccatatcaga ctcctgagtg gtttttgatt acaaacacca acaatcttag taaataacta
     1081 atctaatacc atcaaatggt taatcatgat ttttgttttg tgattctgat taagaaaaag
     1141 aaaataccag acgataagac aaaatatcaa gcgttgtgaa gaaatggtat ttgctctttt
     1201 gcgtctatag tcatgatgtc aaatgaacgc gtttcgacag gaaatcatca tgaataaata
     1261 tcaggcagtt attattggtt ttggcaaggc tggaaaaaca ttagccgtca cgctggcaaa
     1321 agcaggttgg cgagtggctc tcatcgaaca atcaaatgca atgtatggcg ggacctgtat
     1381 taatatcggc tgcatcccaa ccaaaacatt ggttcatgac gcacagcagc acacagattt
     1441 tgtccgtgcc atacagcgta aaaatgaagt ggttaatttt ttacgtaata agaattttca
     1501 taatcttgcg gatatgccca atatcgacgt gatcgacggc caggcggagt ttatcaataa
     1561 tcatagcctg cgtgttcatc ggcctgaggg aaatctggaa attcatggcg agaaaatttt
     1621 tattaatacc ggtgcacaaa ccgtggttcc gccaattcct ggaattacca ccacgcctgg
     1681 tgtatatgac agcaccggat tacttaatct aaaagaattg cctgggcatt taggtatttt
     1741 gggcggcgga tatattggcg ttgagttcgc ctctatgttc gctaattttg gcagcaaggt
     1801 aaccatttta gaagcagctt cgctgttttt gcctcgggaa gatcgggata ttgctgataa
     1861 tatcgcgacg attttacgcg atcagggcgt cgatattatc ctcaatgccc atgtggagcg
     1921 aatcagtcac aatgaaaatc aagtgcaagt gcatagcgag cacgcccaac tggcggtgga
     1981 tgcactgtta atagcttccg gtcgtcaacc ggctaccgct tcgttacatc cagaaaatgc
     2041 cggtatcgca gtaaacgagc gcggggcaat tgtcgttgac aagcaattac ataccaccgc
     2101 agacaatatt tgggcgatgg gagatgttac cggcgggctg caatttactt atatttcact
     2161 ggatgattac cgcattgtgc gtgatgagtt gctgggagaa ggcagacgta gtactgatga
     2221 tcggaaaaat gtgccttatt ccgtattcat gacaccgcca ctgtccaggg ttggtatgac
     2281 agaagaacag gccagagaga gtggtgctga tattcaggtg gtgacattgc ctgtagctgc
     2341 aattccgcgt gccagagtga tgaatgatac tcgtggggta ttaaaagcga ttgttgataa
     2401 taaaacccaa cgtatattag gggcatcact gctgtgtgtt gactcccacg agatgatcaa
     2461 tatagtgaaa atggtgatgg atgccgggct gccttatagc atattacgcg atcagatatt
     2521 tactcatccg tcgatgagcg aatcactcaa tgatctattt tcattagtca aataaactca
     2581 aaatcagacg ccagaacaaa tattctggcg tctcagagaa aagaatctta ttaattccct
     2641 gttactctat aacttcttaa tcacttcatt gatggtattt tatatgttta aaaaatctgt
     2701 tttatttgca acactattat ctggcgttat ggcattttcc accaatgcag atgataaaat
     2761 aattctgaaa catatcagcg tctcgtcagt atcagcatca ccgacagttc tggaggatac
     2821 cattgctgat atagccagaa aatataatgc ttcatcctgg aaagtcacat cgatgcgaat
     2881 tgataataat tcaaccgcaa cagcagtatt gtataaataa ggatgttcac aatggaaaaa
     2941 tacctgcacc tgttaagtcg gggagataaa attggcctga cattgattcg tctgagtatt
     3001 gcaattgttt ttatgtggat tgggttatta aagtttgtcc cttacgaggc agacagcatt
     3061 acaccattcg tcgcaaacag tccactaatg tcgttctttt atgaacaccc ggaagactat
     3121 aaacagtatc tgactcacga aggcgaatac aaaccagaag caagggcatg gcaaacggcc
     3181 aataatacct atggtttttc caacggtctt ggcgtcgtgg aggtgattat tgctctgctg
     3241 gttttggcta atcctgtcaa tcgctggtta ggtttattgg gaggactgat ggcatttacc
     3301 acgccgctgg taacactctc atttttaatc accaccccgg aggcatgggt acccgcattg
     3361 ggtgacgctc atcatggttt cccttattta tccggtgctg gtcgcctggt attgaaagat
     3421 actctgatgc tggcaggtgc tgtcatgata atggcagatt cggcgcggga aattcttaaa
     3481 caacgcagta atgaatccag ttcaacgtta aaaactgaat attgataatc cgaacactgt
     3541 ctgttgttca ctttttcagg cgtcaggccc cctttctgat gatgaagcct gacgccatat
     3601 taccggagat atgacagagt gaagatcaat atcactcccc tgttaaaaga ttcttgtcgt
     3661 ctcaggtaaa atatatgatt aatatcaatc atgtattttt acagcaagac aaccacgaac
     3721 aaattcagga ttaaaatgtt ttaccgactc cccaacataa cccaaatcaa gcgaactaat
     3781 ttgtgccatt tctttctctg tcagtgagaa atcccagata gcaaaatttt cttcgatacg
     3841 ctgttgatgt gtcgatttcg gaataacgac cactccccgt tgaatattcc aacgcagaat
     3901 gacttgagaa atcgattttt gatgggcatc tgcaatactc tgaagcagat tattttcaaa
     3961 gggtttatgt cgtccaccac ctaacggagc ccatgcttca ggctgcacgt tataatactt
     4021 catggttgcc agcgcctctg gttgggcaaa atagggatgc aactcgacct ggttgaccat
     4081 cggtttaact cttaccgttt cacaaaagtt cgccagaaca tgaggataga aattggaaac
     4141 cccaattgct tttaatttgc cctcttcata ggcatcttcg agtgcacgcc acgcactgaa
     4201 ataatcgcgc atagcctggt gcaataaata gaggtcgaag tactctagtc ccgatttttt
     4261 taatgatgct tcaatacctg ctgctgccgt atcctgattc aacatatcct gcacccacaa
     4321 tttagacgtt ataaataact catctcgggt acataaacct tcagatatag cttcacgaac
     4381 ggcttcaccg acggcatctt cattaccgta taccgctgca gtgtcgatga gtcgataacc
     4441 tgtgcggata gcgttcaaca ctgactgttt gcacacgttt ttatcggtaa cctgaaaaac
     4501 accaaatccc atcatcggca ttttcagatt gttacttaat acggaaaatt ccactgtcag
     4561 tccctccatt aggcatgaac aatgagtcta cgttaaaacg taacctcaaa ttagtacgtg
     4621 gattttgata tcacttatgc aaaaaattca ttaataatgc aggactgaaa cctctctatt
     4681 ttcggggaca acgaagcaga cgctaccagt gcttttgcct tcgcccttgc tgtttttgat
     4741 acacttaggg cccagggcat aacgaaaatg tgcgatatga cgaagaatca atggagaatg
     4801 tgatgatcag gcagaagatt ctacagcagc tcctggagtg gattgagtgc aatcttgagc
     4861 accctatttc aatcgaagat atcgcacaga aatctggcta cagcagacgc aacatccagc
     4921 ttctgttccg aaatttcatg catgtgcctt tgggagaata cattcgcaaa cgaaggcttt
     4981 gtcgtgccgc cattcttgtc cggctcaccg cgaaatctat gcttgatatt gcactctctt
     5041 tgcattttga ttcacagcag tcattcagcc gcgaatttaa aaagttattc ggctgctctc
     5101 cccgtgtata ccgccaccgt gattattggg atctcgcaaa tatcttccct tcttttttaa
     5161 tacgtcaaca gcaaaagacc gagtgtagat tagtcaactt tcctgagaca cctatttttg
     5221 gcaattcatt taaatatgat attgaagtgt cgaataaatc accggatgaa gaagtcaaac
     5281 tgcgacgtca tcatttagcc agatgtatga ataattttaa gacggatatc tatttcgtct
     5341 ccacgtttga accgtcaaca aaatcggtcg atttgctcac ggttgaaact tttgccggta
     5401 cggtatgcaa acaaaccgat tcagagatgc cgaaagagtg gacaattaac cgaggactgt
     5461 atgcctcttt ccgctatgaa ggtgagtggg agcactaccc tgaatgggcg cgaaacctct
     5521 atctgatgga attacccgcc agggggttag ccagagtgaa cggcagtgat attgagcggt
     5581 tttattacaa tgaaaatttc gtcgaaattg atagcaataa cattgtttgc gaaattttta
     5641 tccccgttcg tccggtttag ttttcgctat atctaaatga atttataacg agactactgt
     5701 caggaaattc aataaataat ggcgttgaag atgtcaacgc cattattgtt tattagaatg
     5761 ttactttcat attcttaaaa ttatttcatc gcctcctcgt cctttgccgt atcggcatcc
     5821 accgcggtaa tggtcaccgt cactggcttc atttcgtacc cgccataggt cagggcgtta
     5881 aatgtgaacg tatagacacc aggcttgctg gtactaaatt tacgcgtgat ttttccgctc
     5941 acgaaatggt catttttcga cggcaggaac tgataatcct tttccgtcac cccttccggc
     6001 gtctgaatgt cgacccacat attcccgcca acggggttgt ttgccgacat cagtgtcacc
     6061 tccagcttcg cagtcgattt cccatccgcc accagagatt gcatatcaga tgatgcggtg
     6121 agtaccgcgt ttttatcatc cgcaacgaat ttagattcgg cagttttcgt tgatacctga
     6181 ccgttggtct gactcacttg cgccttgagt gtgtatgtcg ccgcaacagt agtggtagcg
     6241 gtgaaaatag cctgcccttg ctcattcgtt gtcgcagtgc cattcggttc caggtttaac
     6301 gaagccgggc tggcagtcag cgtcacttcg ctatctttca ccaaattgtt attggcatca
     6361 gtcaccgtaa ctttatacgt ttgtttggct ttgccgtccg ccagcgcata gttgcccatc
     6421 acctcaatac cggagacggt tgcggtagcc ttatcggcta caaacgttat agtctgcgta
     6481 tccgaaggct ggctattccc cgccgatgcc gtgatctcat acgttccggc agtcactgaa
     6541 accacctgca actctgcttt cccctcaccg ttggctttca cccagacatt atcgcctgta
     6601 agcggcttga caccccgggg cagattaaag gtcaccaggc tccctggaac agggttacca
     6661 tttttatcct caacggtcgc cgtcagcgta aaggcatctt tgccgtttgc cacttttggc
     6721 gcggcgctat caacctccag actcgcctga ccggtactga aatccgcctt gaatttcacc
     6781 gtaaccgtct tctgagcatt attcactgag gccgtgatgc tgtgttcccc ggcaaccgtt
     6841 gacatcagct caatgtcggc tttacctgcc gcatcggttg ttaccgtatt ccccgtcttg
     6901 ctggtcacac ctttcggcag agtcagcgtc acattttgtc cctgcaacgg gttaccatag
     6961 gtgtccacaa cggtcagggt tacctggtta gtcgattgtc cattagccag ctggttatca
     7021 accttcaccg tcatatcacg aatctcagcc ttagatgcgt caccagcaac attcagcacc
     7081 agtggctgag caacggcatt ttggccgttc actcgcggca tcacagacaa ctggcccgcg
     7141 gcagatccca gagtcaaggt cgccacgtag accccattac ttgtttctgt ccagtctcct
     7201 gttgaaggtc gctctgtccc cgtgctggcg gcaccgctaa acactggtgc atctggcttc
     7261 atcccggtga ccgggttccc gtacgcatcc ttcacggtga aggtgagttc cgtcgtcgtt
     7321 ttgacggtcg gagccttatt gtccgcaaca agcgtagagt tcgctgatga catctctccg
     7381 gcgatgaccg tcaactgcgc ggcttcggtg gctgctggct ggccgttgaa tagcggcatg
     7441 acacgaagct cgcccgtctt tccgcctgta gttaacgtag caacatagga accgtcacct
     7501 ttttcggtcc aactggaaac ggtcgcccct tcagaggcgg tccccgtcaa acttgccgac
     7561 aacgaaagac cactgatagc gttgccatgt gcatcttttg caataagcgt cacggttgta
     7621 ctttcgccgg cttttacgtg atcttctgcg acagagactt tcgactggtt tgaagataac
     7681 gcatcagcca ccacggttac ttttgccgca tttgctgccg catcctgtcc atttagcttc
     7741 ggcataactt ctaattcacc cgccgtagag ccgagagtaa tctgcgcagt ccacgtccca
     7801 tcaccattat ttgtccagcc cgatgccgta gaacctacag cagcggcacc cgctaatgac
     7861 ggcgcttccg gcgtgaggct ggtcacaggg ttgtcatagg catcttttac cgtccagatt
     7921 gccgtaactg tacccccaac caccggttta tcaggattca gggtgatgga cgaatgtgct
     7981 gcatcaagcg gcccggcaac aaacttcagc gtttgttgta atgtgcccag ctgatactgc
     8041 tcgctgaaag cacgcacaac aacgtttccg gcacgggtcg aagaaaccgt agcgctgtaa
     8101 acgcctagtt ttatctccga aattgtacca atggtgacac cattagtgtc ttgcggaaca
     8161 aatcgcaagc ggctggcctc tcccgtcacc gggttaccat cagtatccac cgcagtcagc
     8221 gtcagcgtgt aggcttgctg accatcagca accacgtcac ctgacggctc gttagcgctc
     8281 agggtggagt ttgccacatc catcatcgtg gcccgcagtt ctgcagtgac ggttttgctc
     8341 atgccatcaa cactaacagt aatcgttgcc tcacctgact gcgttccggt agtaaagaca
     8401 gactgataca cccctgcttc agtttcggtg aactcaccca gtgttggctt tgcctgtgat
     8461 ttagtggcct tcagggtacg agtcacaata tttccagccg gtttgaaagt tagttcagtc
     8521 ttgatctgat ctttcatgcc cgtgactggc tggccctcgg cgtcgcgcag agacagcacc
     8581 agcggtcttt gctcattacc gttagcaagc atttgaatac ggctctgacc gtcaagcgtt
     8641 aacgccgtgc gatcggcact catacctgct ccggtaatga ccacctctgt ctgcacgcgt
     8701 tttgaggcat tgcctttgtt atcgtaggca accgcagaaa tcgcataata attgtctttg
     8761 cctggacgat aagccgggag cgttacttgc cactgactac cctgaccggt aattttgcca
     8821 ccttcagcca gtaatgacgg cgcttcccac tgcacatttt tcagtccgtg agttgctttg
     8881 ctgaccacaa gccccaggga aagtgtctga ccgcccttac cttcaatacg ctcaggcaga
     8941 gcaatacgga tcacttcaga tttgcggtac tcaagaacga tgttgttatt acgctcaacc
     9001 aggtcatagc ggctgcctgc cagcattcga cgctcgcgaa tgctgtctgt atcgagttgt
     9061 ttctccagag gttcgccaat ccgataatta acttccaggc caaagcgagt gtcattctca
     9121 ccgctcttgc cctgcttatg cccggcgctc agtgtcagaa gaggcactgg cgtataggtc
     9181 acctcggcag aaatagcatg cgggtctttc tggcgcttat ctttaccaaa cagcccgact
     9241 tcatcgccat aatactgttc atacatcagg cttgcgccaa gctgcggcca ggcaggtaaa
     9301 tagccctctg cgcggatatc ccaaccattc gccgggcgtt cctgataatc ctcaatatcc
     9361 ggcgattttt tccagccaga agcccgaata taaccattgg cgctcagttt cagataatcg
     9421 cgccagtatt ccgcaccaac accaatgcgg gtatgactac gggataaatc atgatcgata
     9481 aaagtattca cccccgccat ccagtcattt cctgaaaaat gacgccagcc aaaaccaata
     9541 tttgactgag tacgatcgtc tgtacgatgt attgccccct gagtgaacaa catatttgtc
     9601 ggcgtatcat aaatcggata aagcatttcc agcgaagaat ccttcagcga gaaatcttta
     9661 tcgacattca gtttgacgcg cgctgtacca tatttcccga gccactcctg tatttcctgg
     9721 ttagctttag cggtggccat tccggtaata aaattacgtg tcgcatcgct atctggctga
     9781 ctgcttaaaa atgtcccggc atttgcggca aacgacgcga catttttctc cacattatta
     9841 tcagcagtta ccgtggtatt ttccatgctc aaccgtggct gaaccgcatg ctgcgcatgt
     9901 gccgccatta ctggggtaaa ggtgacagcg agtggaaaaa gaacctgaac agagatattt
     9961 gcccacgcca cgcagcgggc cagaactgaa taacgaaata gtggttgttt atgacctgtt
    10021 ttataacgtg acattgagat aatttcctga tgaacgaatt aacgaacata ggcagacaca
    10081 aataatggcg tctgcttgtc agaaaaaccg gagatttccc ggtttccttt tccgcagaaa
    10141 aggaaatacc tttatgtaag aaagtacacc gccacctaat atatccagtg cctgtagcca
    10201 ctcatcaggt ggttgggcgc gggattatat ttttatttat gggtaaaaat ttagcttttt
    10261 gtgcaagtca ttaaatagat acctaagtta tatttcttac tctgcggaca gtgaataatt
    10321 cattattcag agtgaataat aatgacctgt ctttaattta ccacgattgg caatacatcg
    10381 aagtaggtat atacgccagg ataaaccaat atatttttta tggatattca ttttcattca
    10441 tcagactgta aattgcggag atatcgaact tgcactaccc atcgcaacac tcaaacagat
    10501 ttatgaattt cgttcataac ccctgacaaa tcctgccgtc taatcgagct tttcccttta
    10561 cgttatgctt ctcatgaacg ataacacaac ttgttcatga attaaccatt ccggataaac
    10621 tatgggatca aattcaggca tcgaaaggaa ctgactggtt tccgttaatg aattaaatat
    10681 ctattactct cccgagagta aaactatcaa accaatgagt aaccgataca gcctcgcaaa
    10741 ttctttcaga taattaaccc gtcactgcgg cacgttctct cgtaaggcgt gcctgcagtg
    10801 taatccagta tgtatccggc atagttgacg gtttaataga actcaattcc tctcccaggt
    10861 caccaacaac gatatcttgt ataacacccc aggagatacg acattatgat gagatgcgat
    10921 atgctcctca gcgccaatca acgcccggac tgggccagtc ttgagatcag actcattccc
    10981 ggaactgact tcaggcgaca tatatcaatg acgttggtgc aataaaagat agccgatgta
    11041 atacatccat tcaaatttgt gatacatgtc aaaaaattgg ttgaccaaac tcgttattta
    11101 tataagggca cttacgaagt gcgctctttt ttaaagcgag gaagtaccaa tgaaagagaa
    11161 taaagtacaa caaatcagtc ataaactgat taatatcgtt gtttttgtcg caattgtaga
    11221 atacgcctat ttatttttcc atttctatta ataacggcaa taaactgttc acttcagtga
    11281 tatttaaaat atgcatcctc tccctttttg taagtaatta ttatatccgt gggagaggaa
    11341 tacacgctgt caggtaatca atcatactgc gataaatcat cggccagtaa agtggagata
    11401 acctccattg tcgaaaaatc catactctca gcgaaaccat catcaatcac tcatccaggc
    11461 gtttatggga gcgtcgccaa cggctgctaa caatgccaga cttccccgtt ggggaaattc
    11521 cacataccaa aaatgggcac agggattggg tgttgaaatg atccggatga gcatgtatct
    11581 ttatggttat gttataacat aacaggtaaa aatgatgaag cccaatatcc atcctgagta
    11641 tcgtactgtg gtgttccacg acaccagtgt tgatgagtac tttaaaatcg gctcgactat
    11701 caaaacagac cgtgagattg agctggatgg cgtaacgtat ccatacgtga caattgatgt
    11761 ctcttcgaaa tcgcacccgt tctatacagg gaagctgaga acagtggcat cagaaggaaa
    11821 tgttgcacgg ttcacccaac gttttggtcg ttttgttagc acgaaaaagg gggcatgatg
    11881 aaagtcctta actctctgcg taccgcaaaa gaacgccatc cagactgtca gattgtgaag
    11941 cgaaaaggac ggctgtatgt gatttgtaaa tctaatccac gttttaaggc cgttcagggt
    12001 cgtaagaaaa aacgttgatt taaaattcga cgaattaacg atatttgtct gattaataat
    12061 cagaccggat taatgttggt gtatttatta caccagcatt aatttttctg gggatgtatc
    12121 ttcctgttca tttaaggtca actgcctgac gtttctctcc gaatattcca ttatattaat
    12181 gttgacttgt tgaccagctt cgccctgtat gctggcatca accctctttt agactgaaca
    12241 cgccactcag tttcctccct ttgcgacgca gcctgcattt acactcaaac tgttaagatg
    12301 ataaatgtgg taaatctgtt ggtactgaca taaaaacgtt tacgccacag gaacggcctg
    12361 atccaccggt aaccccatcg ccgacgttcg agtgccagtt agagtaacgc gcacagatga
    12421 ctgaatgcag tgccctggca aagaggccat cgttcctgtg acaactggca gtcttcgttt
    12481 aacttcactt aatttggctc ttagggggct taccggacag atgacgtact tatacctgtt
    12541 taatttttca tcacttattg ggatgaacac ccataaccat tttgtgcggc atgttaatcc
    12601 attaaaacac cttactgatt ggcaaatcat ctttaattat gactcatgat agttttatat
    12661 tctatttctt atcatttaaa cttgtttttt tactagtcca ttacacaaca cattaagact
    12721 attcctaaca cctcagggca aagttcctgg ctaatataaa atgcaagtaa gaattgaacg
    12781 ttatattgcc aataacctta tgaaactgaa tgtctttttc ttcttatcaa aaaagcaata
    12841 ttttcagttt ttctaaatat tgacttaacc attgaattcc ttttctgttc acatattgac
    12901 actcatcagg aaaaaaacat aaatttaaac ctaatcgaaa taattaaaac ttaatctcgt
    12961 ttaacctata ttgatatgtg ctacgtatct tatttacttc cgatttacta aagaaactga
    13021 atgtacctgt aaaaattaca ggtttggaaa gtagtgacat ggcaaaatga ttacagcagg
    13081 gactatgagg ttaaaaacca tatggaatgt caaaaccgtt ctgataaata catctggtct
    13141 ccccatgacg cctacttcta taaaggacta tctgaactga ttgtggatat cgacagatta
    13201 atttatctat cgctggagaa aatcagaaaa gatttcgtgt ttatcaatct caatacggat
    13261 tctttaactg aatttataaa ccgtgataat gagtggttat ccgcggtaaa ggggaaacag
    13321 gtcgtattga ttgcggccag aaagtcagaa gccttagcaa attattggta ttacaacagc
    13381 aatattaggg gcgtggtata cgctggactg agtcgtgata ttagaaaaga actggcctat
    13441 gtgattaatg gcaggttcct gagaaaagat attaagaaag ataaaatcac tgaccgagaa
    13501 atggaaatta tccgcatgac ggctcaggga atgctgccta aatcgattgc cagaattgaa
    13561 aattgtagtg taaagacagt gtatacccat cggcgtaatg cagaggccaa gctgtactca
    13621 aaaatatata agttggttca gtaaactcca ggcaagttag ttttaaaaaa tgactcactg
    13681 ggacatcacg tcctcaattc aactcgggaa gaaatgcaat gaaaaaaaag gttctggcaa
    13741 tagctctggt aacggtgttt accggcatgg gtgtggcgca ggctgctgac gtaacagctc
    13801 aggctgtagc gacctggtcg gcaacagcca aaaaagacac caccagtaag ctggttgtga
    13861 cgccactcgg tagcctggcg ttccagtatg ccgaaggcat taaaggtttt aactcacaga
    13921 aaggtctatt tgacgtggct atcgagggtg actcaacggc taccgccttt aaactgacct
    13981 cacgtcttat caccaacacc ttaacccagt tggatacctc aggttctaca ctgaatgtgg
    14041 gcgtggatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg atcgataccg
    14101 ccaacggcgt actgggcggc aaccttagcc cgctggctaa cggttacaat gccagcaatc
    14161 gtaccaccgc acaggatggt ttcactttct ccatcatcag cggcaccacc aatggtacca
    14221 ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac gttagcgtac
    14281 agttcgacgc gacctggacc agttaatctc tctgatgtac cagcaggggt agcccccccc
    14341 ctgctttatt cctggacgga attgttatga aaaagcacct tctgcttctc gctctgctgt
    14401 tatccggaat atctccggcc caggcgctgg atgtcggcga tatatcatcg tttatgaaca
    14461 gtgacagcag cacgctgagc aaaacgatca aaaacagtac cgacagtggt cgcctcatca
    14521 atatccgtct cgaacggctc tcttcaccgc ttgacgacgg gcaggttatc gcaatggaca
    14581 agccggatga gttgctactc actcccgcca gcttgctgct acccgcccaa gccagcgaag
    14641 tgatccgctt cttctataag ggaccagcag atgaaaaaga gcgctactac cgcattgtct
    14701 ggtttgatca ggccctcagt gatgcgcagc gcgataatgc caaccgcagc gctgtggcca
    14761 ctgcttccgc ccgcatcggc accattctgg tcgtcgcacc ccgccaggca aactaccact
    14821 ttcagtacgc caacggctcc ctgacaaata caggaaatgc gacgctgcgg atcctcgctt
    14881 acggcccctg cctgaaagcc gccaatggta aggagtgtaa agagaattac tacctgatgc
    14941 cgggcaagtc gcgtcgtttt acccgcgtgg acacggcgga taacaaagga cgggttgcac
    15001 tttggcaggg tgataagttc attcccgtga aatagatagc tgtgcagatg gataacgaca
    15061 atgcctttac gacggttctc cccaggactg aaagcccagt ttgccttcgg catggtcttt
    15121 ttgttcgttc agcccgatgc cagcgctgct gacataagtg cgcagcaaat aggtggggtg
    15181 attattccgc aggccttcag tcaggcgctc caggacggca tgagcgtccc gctttatatt
    15241 catctcgccg gtagccaggg tcgccaggac gatcagcgaa tcggcagcgc ttttatctgg
    15301 ttggatcatg gacagctacg catccggaaa atacagctgg aagagagtga agataacgcc
    15361 agtgtcagcg aacaaactcg acagcagctg atggctctgg cgaacgcccc gttcaatgag
    15421 gcccttacca tccccctgac tgacaacgcg cagctggatc tcagcttgcg ccaactgctg
    15481 ctgcagctgg tggtcaagcg cgaagcgctg ggcactgtac tacgctcacg tagcgaagac
    15541 atcgggcagt ccagtgttaa caccctcagc agtaatctga gctataactt cggcatctat
    15601 aacaaccagt tgcgtaacgg cgggagcaac acatccagct atctgtcgct gaataacgtt
    15661 actgcactgc gcgaacatca tgtggtgctc gacggctcgc tgtacgggat cggtagcggt
    15721 caacaggaca gtgaattata taaagcgatg tatgaacgcg attttgccgg tcaccgattt
    15781 gccggtggaa tgctcgacac ctggaacttg cagtccttag ggccgatgac cgccatttca
    15841 gcagggaaga tttacggcct ttcctgggga aaccaggcca gctccaccat cttcgacagc
    15901 agccagtcag ccacgccagt gatcgccttt ttaccggcgg cgggcgaagt acatctcacc
    15961 cgtgatgggc ggttactaag cgttcagaac ttcaccatgg gcaatcatga agtggatacc
    16021 cggggtctac cgtacggtat ttacgatgtg gaagttgagg tgatcgttaa cggtcgcgtg
    16081 atcagcaaac gcactcaacg ggtcaataag ctgtttagcc gggggcgcgg cgtcggtgca
    16141 ccactggcgt ggcagatatg gggcggtagc tttcatatgg atcgctggtc ggaaaacggg
    16201 aaaaagacgc gaccagctaa agagagttgg ctggcaggtg cctcgacctc cggctcactg
    16261 agtacgctta gctgggcggc aacgggatat ggatacgata atcaggcggt gggtgaaacc
    16321 cgtctgacgc tgccgcttgg gggagcgatc aacgttaacc tgcaaaatat gctggccagt
    16381 gacagctcat ggagcaacat cgccagcatc agcgccactc taccgggagg ctttagttcg
    16441 ctgtgggtta accaggaaaa aacccgcatt ggcaatcaat tgcgacgtag cgatgccgac
    16501 aaccgtgcaa tcggcggcac actcaacctg aactcactgt ggtcgaagct gggcacattc
    16561 agcatcagct acaatgatga ccgccgttac aacagccatt attacacggc agattactat
    16621 caaaatgtct acagcggtcc ctttggttcg cttggcctgc gggccggtat tcagcgctat
    16681 aacaacggcg acagcaacgc caatacaggg aaatatatcg ctctcgatct ctcgctacca
    16741 ctgggcaact ggtttagcgc agggatgact catcaaaacg gctacaccat ggcaaacctg
    16801 tcagcacgca agcagtttga tgaagggacc attcgcactg ttggtgccaa tctgtcacga
    16861 gccatctccg gcgataccgg tgatgacaaa accctcagcg gtggggcgta tgcacagttc
    16921 gacgctcgtt acgccagcgg aacgctgaac gtcaatagcg cggcggacgg ctacgtcaat
    16981 accaatttaa ccgccaatgg cagcgtcggc tggcagggta aaaacattgc tgccagcggg
    17041 cggactgatg gcaacgctgg ggtgatattc aacaccgggc tggaggacga cggtcagatc
    17101 agcgccaaaa tcaacgggcg gattttcccg cttaacggca agcgtaacta tctcccgctc
    17161 tctccctatg gaagatatga ggtggagtta cagaacagca aaaactcact cgacagttac
    17221 gatatcgtca gcggccgcaa aagtcatctg actctctatc caggcaatgt cgctgtcatt
    17281 gagccagagg tgaagcagat ggttaccgtc tccggccgta tccgtgcgga agacggcaca
    17341 ctgctggcta acgcacggat taacaaccat atcggccgaa cccgaaccga tgaaaacggc
    17401 gagtttgtca tggatgtgga caagaaatac cccactatcg attttcgcta cagtggcaat
    17461 aaaacctgcg aagtggcact ggaactcaac caggcgcgcg gtgccgtctg ggtcggagat
    17521 gtggtctgca gcggcctctc atcgtgggcg gcggtgacgc agacaggaga agagaatgag
    17581 agttaaccta ctgatagcga tgataatctt tgcgctaatc tggcctgcaa ccgcgctcag
    17641 agctgcagtg agcaaaacaa cctgggcgga tgcaccggca cgcgagtttg tgtttgtcga
    17701 aaacaactca gacgacaact ttttcgtcac tcctggcggg gcgctggatc cgcgcctgac
    17761 cggtgccaac cgctggaccg gtttaaaata caatggttca ggaaccatct atcagcaaag
    17821 cctcggctac attgataacg gttacaacac cggcctttat accaactgga agtttgatat
    17881 gtggctggaa aattcaccag tttcatctcc tttaactggc ttgcgctgca tcaactggta
    17941 cgctgggtgt aatatgacca ccagtcttat cctgccgcaa accaccgacg ccagtggatt
    18001 ttatggcgcg acggtgacca gcggcggcgc gaagtggatg cacggcatgt tgtcagacgc
    18061 gttttaccag tatctgcaac aaatgcccgt cggcagcagc tttacaatga ccatcaatgc
    18121 ctgccagacc tctgtgaact atgacgccag cagcggcgca cgctgtaagg atcaggcctc
    18181 cggcaactgg tatgttcgca acgtcaccca tacgaaagca gcaaatctac ggttgataaa
    18241 tacccactcg ctggcggaag tatttatcaa cagcgacgga gtaccgactc tgggcgaagg
    18301 gaacgccgac tgccggacgc aaaccatcgg cagccgttca ggattaagtt gtaagatggt
    18361 taactatacc ctgcaaacaa acggactcag caacacctca atccatatat tcccggcgat
    18421 cgccaactcg tcgttagcct cggccgtcgg ggcgtacgat atgcagttca gtctgaatgg
    18481 cagttcatgg aaaccggtga gcaatactgc ctattactac accttcaacg agatgaagag
    18541 cgcagactcg atctatgttt tcttctcgag caacttcttt aagcagatgg tgaacctcgg
    18601 gatcagcgat atcaacacca aagatctatt caactttcgc tttcagaaca ccacatcacc
    18661 ggagtctggc tggtatgaat tttctacctc caacacgctg attatcaaac cccgtgattt
    18721 cagcatcagt attatctccg atgaatatac tcagacaccg tcgcgggagg gatatgttgg
    18781 cagcggcgag tcggcactcg atttcggcta tatcgtaacc accagcggta aaacagctgc
    18841 cgacgaagtg ctgatcaagg tgaccggacc cgcgcaggtg attggcgggc gctcctattg
    18901 tgtcttcagc tccgatgacg gtaaggcgaa agtaccgttc ccggcgacgc tttcctttat
    18961 tacccgcaac ggagctacaa aaacctacga tgccgggtgc gatgatagct ggcgtgatat
    19021 gaccgatgcg ctgtggctga ccacaccgtg gactgatatc tctggcgaag tggggcagat
    19081 ggataagacc acagtcaaat tttcgattcc aatggataac gccatttcgc tgcgtacggt
    19141 agatgataac ggctggtttg gcgaagtcag cgcttcagga gaaattcatg ttcaggcgac
    19201 gtggcgtaac attaactaag gccctgctga cagcggtctg tatgctggcg acacctttga
    19261 cacaggcgat ttcggtcggc aatctgacat tttcgctgcc gtccgagact gactttgtca
    19321 gcaaacgtgt agtgaataac aacaaaagcg cacggatata ccgtattgcc atcagtgcta
    19381 ttgatagccc gggcagcagt gaattgcgca cccgaccggt ggatggtgaa ctgcttttcg
    19441 ccccccgcca gctagcgttg caggctggtg agagcgagta ttttaaattt tactatcatg
    19501 ggccacggga taaccgcgag cgctactacc gggtctcatt tcgcgaggtc cccactcgta
    19561 accagacaag acgtagccca accggcggcg aggtcagcac ggagccggtg gtggtgatgg
    19621 ataccattct ggtagtacga ccacgtcagg ttcagtttaa atggtccttc gacaaggtga
    19681 caggaacggt aagtaacact ggcaacacat ggtttaagct actgattaag ccagaatgtg
    19741 attcgaccga agaggaaggc gatgcctggt atctacgtcc gggagacgtg gttcatcagc
    19801 ctgagttacg tcagccgggg aatcattatc tggtctataa cgacaaattc attaagatta
    19861 gcgattcctg tccggctaag cccccttcgg cggactaaga cttatccatc ggtcgaggga
    19921 agaattccac ctcagagccc caaaaatcgt ttaaatgatg tggaagcgat cgtcaatatc
    19981 acctgtaacg atgggctttc tcatatgcac accatagtgt tcaggagaca cggaaaggta
    20041 tcaggcagcc tgctaacgag tggtttaatc acgaattagt acgtaaaatc ggtaacggct
    20101 ggaaatcatt caatactcac actatcgaaa gttccccagc caaccgcggt acgttcttac
    20161 atacgatgta ccgctgttct ctttacgatt tttagctgta ctggtgaatt atgagcaatc
    20221 tgaatccatg catgacgtgt ggtgcctgtt gtgcattttt ccgcgtctct ttttactggg
    20281 ccgaagccga cgatgctggc ggcactattc ccgccaggct cactgaacaa atatcccctt
    20341 ttcaccgatg catgagcggt accaatcaga aaaacccccg atgtattgcc cttgcaggaa
    20401 ccccgggcaa aaatgcctgc tgcacgatat ataaaaatcg atcgtccaca tgcagagaat
    20461 ttgccatgtc tggtgagaac ggagtcgtca atgaggcttg caatcgtgca agggctaaat
    20521 acgggctgac accgttataa acatacaata attaattgca ctgccccgcc agcgataata
    20581 gcggggcttc gtttttcagg ggtaacaaaa cccgatactt cttctatttg ccagcaacaa
    20641 tgcctctctt ctgtagcgtt cttcggatct acatcatcct gaagtagcgc gatatactca
    20701 ctgactatct gaatcagaat ataaaaagca atgtttttaa cctataaaaa tggcgctgta
    20761 tttgcgccat ttttatcatt caatgcatta tctgtttgag cctaaaggga tctcagggtc
    20821 tggctcatga gtaattctgt ttcgtaaatc tctgcgaata atttcaatag accagaacca
    20881 gactaaatgt ccaaaaattt cagaaacatt ctcataccac gggagatcaa acagaggtgg
    20941 cgtcagtccc atgagaggga atgaaatcat atgaacaaaa agttgggcta aagcacctgc
    21001 cagtaagccc tgccagagtt taatttttgg aaatacttca gcgaccacac aataaccgac
    21061 agcaaacact atcgaaaaga taatgtgcgt aacaccaacc cagttaaaga catgcccggc
    21121 aaaggtataa acagccgcat tgggatctgt cagccccaac cagtctcgaa gaaaaatata
    21181 cggtggattg agaaaattac gcgagcaatc aatttggcct gcagccctga ttaatgattc
    21241 cgggccacac gctgcattaa acatatccac cgggctacgt ggcggcaatg gaacttcagc
    21301 cccccacttc acgaatgcgg aaacaacgcc agcaatcagc ccaatgaatg cagcaagacc
    21361 ataacgtctg cggttcggtg gagtttgttc aaatatattc atatctaccc tgcttgtacc
    21421 attatgttat acacctcttc aggagtattc ataaaacaag gcaaatgtaa agaactgtat
    21481 tgttttgtat aacaagatag tttcctaatc gccaatgaat ataagctcca tcatttctcc
    21541 ctatttttat attagaagtg acagagattt gcagggtgat gcagagctga aaatcacagg
    21601 tttccttatt ggtttttgca tcgtacaact aaagcaataa accagctccc atcatcatca
    21661 acatcccggc gaacatgaat ttattagccg ggctcatagc tgcattcgcg ttttaagaat
    21721 aatcctcctg ctgtcgccga ctatgcttaa cgtttaaaaa agcatcagca ctctcgcaac
    21781 gcactcttat tttccccttt agaataccgg aggcctggta tgagcaacca aggcgaatac
    21841 cccgaagata atcgggttgg gaagcacgag ccgcacgatt taagtttgac ccgtcgcgat
    21901 ctgattaaag tgagcgccgc aacagcggcg accgccgtgg tttatcctca ttctacgctg
    21961 gcggcaagcg ttccggcagc tacacccgcg ccagagataa tgcccctgac actgaaggtg
    22021 aacggcaaaa ccgagcagct tgaggtggat acccgaacca cgctactgga cgctttgcgt
    22081 gaaaatctgc atttgttcgg taccaagaaa ggttgcgatc acggacagtg cggagcctgt
    22141 accgtgctgg tcaatggtcg caggcttaat gcctgcctga cgcttgcagt catgcatcag
    22201 ggggccgaga tcatcaccat tgaaggcctg ggttcgccag ataatcttca ccccatgcag
    22261 gcggccttta tcaagcatga tggcttccag tgcggctact gcacctccgg gcaaatttgc
    22321 tcatcagtag cggtgctaaa agagattcag gacggcattc ccagtcacgt cacggtcgat
    22381 ttggtttccg ctccagaaac aactgccgat gagatccgtg aacgtatgag cggcaacatc
    22441 tgtcgctgtg gtgcatacgc taacatcctt gccgccattg aagatgctgc gggggagata
    22501 aaatcatgaa ggcgtttacc tatgaacgag tgaacacccc agccgaggcg gcacttagcg
    22561 ctcagcgcgt acccggcgca aaatttatcg cgggcgggac caatctgctg gacctgatga
    22621 agctggaaat tgaaacgccc acccacctta tcgatgtgaa cggcctcggg ctcgataaga
    22681 ttgaagtgac cgacgcgggt gggctgcgca tcggcgcact ggtacggaac accgacctgg
    22741 cggctcacga gcgcgtgcgt cgtgattacg cggtactctc ccgcgccctg ctcgctggcg
    22801 catctggtca gttacgtaat caggcaacca ccgcaggtaa tctgctccag cgcacgcgct
    22861 gcccctattt ttacgacacc aatcagccct gcaataagcg cctgcccggg agcggctgcg
    22921 cggcgcttga aggctttagc cgtcagcacg cggtggtagg cgtaagtgaa gcctgcatag
    22981 ccacccatcc gagcgatatg gcggtcgcaa tgcggttgct ggatgcggtg gtggaaacca
    23041 tcacgccgga gggaaagact cgcagtatca cactggctga tttttatcac ccaccgggga
    23101 aaacgccgca cattgaaacc gccctgcttc ccggtgagct tatcgttgcg gtgacgttac
    23161 ctccaccgct cggcggaaaa catatctacc gtaaggtgcg cgatcgcgcc tcctacgcct
    23221 ttgccctggt atcggtcgcg gcgattattc accctgacgg cagcgggcgc gtcgcgctgg
    23281 gcggagtagc acataagccc tggcgcattg aggctgcgga tgctcagcta tcccaggggg
    23341 cgcaggccgt atatgacacg ctgttcgcca gcgcccatcc caccgctgaa aacaccttta
    23401 aactcctgtt ggcgaagcga acgcttgcct ccgtactggc tgaagcgagg gcacaggcat
    23461 gaaatttgat aaacccgcag gggaaaacac gatcgatcag ctgaaggttg tcggtcgtcc
    23521 ccatgaccgc atcgacggac cgctgaaaac taccggcacg gcacgctacg cctacgaatg
    23581 gcatgaagaa gcccccaacg ccgcctatgg ctatatcgtc ggttccgcca ttgccaaagg
    23641 acgcctcacc gcccttgata cggacgccgc gcaaaaagcg ccgggcgtac tggctgtcat
    23701 taccgccagt aacgccgggg cactcggcaa aggcgacaaa aacaccgcca gactgttagg
    23761 cggccccact attgagcact atcatcaggc cattgcgctg gtagtggccg agaccttcga
    23821 acaggcgcga gcggcggcct cgctggtgca ggcacactat cgccgtaata aaggagctta
    23881 ctccctggcg gacgaaaaac aggccgtcaa tcagccgccg gaagacacgc ccgacaaaaa
    23941 cgtcggtgac tttgacgggg ctttcacctc cgctgcggtg aagattgatg ctacctacac
    24001 gaccccggac cagagccata tggcgatgga gccgcatgcc tcgatggccg tctgggatgg
    24061 aaataagctt accctctgga cctcaaatca gatgattgac tggtgccgca ccgatctggc
    24121 aaaaacgctg aaagttcccg tggagaatgt gcgtattatc tccccgtata tcggcggagg
    24181 gtttggcggc aagctgttcc tgagaagcga tgcgctgctg gcggccctcg ccgcccgagc
    24241 ggtgaaacgt ccggttaaag tga
//
LOCUS       DAZGXZ010000073        24242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000073 DAZGXZ010000000
VERSION     DAZGXZ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.073"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            253..339
                     /gene="leuL"
                     /locus_tag="ACHKJF_003031"
     CDS             253..339
                     /gene="leuL"
                     /locus_tag="ACHKJF_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP0950349.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            432..2003
                     /gene="leuA"
                     /locus_tag="ACHKJF_003032"
     CDS             432..2003
                     /gene="leuA"
                     /locus_tag="ACHKJF_003032"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP0950350.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2003..3094
                     /gene="leuB"
                     /locus_tag="ACHKJF_003033"
     CDS             2003..3094
                     /gene="leuB"
                     /locus_tag="ACHKJF_003033"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP0950351.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3097..4497
                     /gene="leuC"
                     /locus_tag="ACHKJF_003034"
     CDS             3097..4497
                     /gene="leuC"
                     /locus_tag="ACHKJF_003034"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP0950352.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4508..5113
                     /gene="leuD"
                     /locus_tag="ACHKJF_003035"
     CDS             4508..5113
                     /gene="leuD"
                     /locus_tag="ACHKJF_003035"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP0950353.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACHKJF_003036"
     CDS             complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACHKJF_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFP0950354.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACHKJF_003037"
     CDS             complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACHKJF_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP0950355.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6662..8317
                     /gene="sgrR"
                     /locus_tag="ACHKJF_003038"
     CDS             6662..8317
                     /gene="sgrR"
                     /locus_tag="ACHKJF_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP0950356.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLLYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8351..8450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8481..9464
                     /gene="thiB"
                     /locus_tag="ACHKJF_003039"
     CDS             8481..9464
                     /gene="thiB"
                     /locus_tag="ACHKJF_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP0950357.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9440..11050
                     /gene="thiP"
                     /locus_tag="ACHKJF_003040"
     CDS             9440..11050
                     /gene="thiP"
                     /locus_tag="ACHKJF_003040"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP0950358.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11034..11732
                     /gene="thiQ"
                     /locus_tag="ACHKJF_003041"
     CDS             11034..11732
                     /gene="thiQ"
                     /locus_tag="ACHKJF_003041"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP0950359.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACHKJF_003042"
     CDS             complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACHKJF_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0950360.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACHKJF_003043"
     CDS             complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACHKJF_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP0950361.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13913..15613
                     /gene="araB"
                     /locus_tag="ACHKJF_003044"
     CDS             13913..15613
                     /gene="araB"
                     /locus_tag="ACHKJF_003044"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP0950362.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15624..17126
                     /gene="araA"
                     /locus_tag="ACHKJF_003045"
     CDS             15624..17126
                     /gene="araA"
                     /locus_tag="ACHKJF_003045"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP0950363.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17295..17990
                     /gene="araD"
                     /locus_tag="ACHKJF_003046"
     CDS             17295..17990
                     /gene="araD"
                     /locus_tag="ACHKJF_003046"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0950364.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18065..20416
                     /gene="polB"
                     /locus_tag="ACHKJF_003047"
     CDS             18065..20416
                     /gene="polB"
                     /locus_tag="ACHKJF_003047"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP0950365.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20581..23487
                     /gene="rapA"
                     /locus_tag="ACHKJF_003048"
     CDS             20581..23487
                     /gene="rapA"
                     /locus_tag="ACHKJF_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP0950366.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSCEYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQIMESLDQAGWRLDALRLIVVTHQ"
     gene            23499..24158
                     /gene="rluA"
                     /locus_tag="ACHKJF_003049"
     CDS             23499..24158
                     /gene="rluA"
                     /locus_tag="ACHKJF_003049"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP0950367.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
BASE COUNT         5750 a         6614 c         6555 g         5323 t
ORIGIN      
        1 aacgcgtgca ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat
       61 atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca
      121 tcgcagcaca attagctaat tttacggatg cagaactcac gctggcggga cgtttttatt
      181 gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct
      241 ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct
      301 tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa
      361 aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa
      421 ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac
      481 aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc
      541 gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat
      601 cggtgcaaac catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg
      661 tggagaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc
      721 atacctttat tgccacttcg ccaatgcaca tcgccaccaa gctgcgcagc acgctggatg
      781 aagtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg
      841 aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag
      901 cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc
      961 cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag
     1021 ccattatttc cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg
     1081 cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg
     1141 gtaactgttc gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg
     1201 tccacaccgc cattaatcac caggagatat ggcgcaccag ccagttagtt agccagattt
     1261 gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc gcacactcct
     1321 ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag
     1381 aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg
     1441 tgaaacatcg catggatgag atggggtata aagaaagtga atataattta gacaatttgt
     1501 acgacgcttt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg
     1561 cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca
     1621 gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc tgcggcgaag
     1681 aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcgataa
     1741 accgcatcac tgactataac gtcgaactgg tgaaatacag cctgactgct aaaggtcacg
     1801 gtaaagatgc tctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg
     1861 gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga
     1921 acaatatctg gcgcgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg
     1981 aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga
     2041 cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg
     2101 ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca
     2161 cgggcagccg ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt
     2221 tggctcggta ggcggcccga agtgggaaca tttaccacca gaccagcaac cagaacgcgg
     2281 cgcgctgctg cctctgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact
     2341 gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcaa acggcttcga
     2401 catcctgtgt gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga
     2461 aggtagcgga caatatgaaa aagcctttga taccgaggtg tatcaccgtt ttgagatcga
     2521 acgtatcgcc cgcatcgcgt ttgaatctgc tcgcaagcgt cgccacaaag tgacgtcgat
     2581 cgataaagcc aacgtgctgc aatcctctat tttatggcgg gagatcgtta acgagatcgc
     2641 cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct
     2701 gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct
     2761 gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa
     2821 cgagcaaggt tttggactgt atgaaccggc gggcggctcg gcaccagata tcgcaggcaa
     2881 aaacatcgcc aacccgattg cacaaatcct ttcgctggca ctgctgctgc gttacagcct
     2941 ggatgccgat gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg
     3001 cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga
     3061 tatcattgcc cgctatgtag cagaaggggt gtaatcatgg ctaagacgtt atacgaaaaa
     3121 ttgttcgacg ctcacgttgt gtacgaagcc gaaaacgaaa ccccactgtt atatatcgac
     3181 cgccacctgg tgcatgaagt gacctcaccg caggcgttcg atggtctgcg cgcccacggt
     3241 cgcccggtac gtcagccggg caaaaccttc gctaccatgg atcacaacgt ctctacccag
     3301 accaaagaca ttaatgcctg cggtgaaatg gcgcgtatcc agatgcagga actgatcaaa
     3361 aactgcaaag aatttggcgt cgaactgtat gacctgaatc acccgtatca ggggatcgtc
     3421 cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac
     3481 tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa
     3541 gttgaacacg tactggcaac gcaaaccctg aaacagggcc gcgcaaaaac catgaaaatt
     3601 gaagtccagg gcaaagccgc gccgggcatt accgcaaaag atatcgtgct ggcaattatc
     3661 ggtaaaaccg gtagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatc
     3721 cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca
     3781 aaagccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtctgcat
     3841 gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac
     3901 gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc
     3961 acctggggca ccaatcccgg ccaggtgatt tccgtgaacg acaatattcc cgatccggct
     4021 tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg
     4081 aaaccgggta ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc
     4141 aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg
     4201 ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg
     4261 gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca
     4321 atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc ctccaccagc
     4381 aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca
     4441 atggctgccg ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga
     4501 gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc
     4561 caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg
     4621 ttttggcgcg catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa
     4681 cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga
     4741 aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt
     4801 taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca
     4861 gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc
     4921 caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa
     4981 aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag
     5041 tattgggctt accttgcagc acgacgacgc cattgccgct tatgaagcaa aacaacctgc
     5101 gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc
     5161 atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta
     5221 cccagtagac agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca
     5281 gaattacccc ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag
     5341 gcattaaatc ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt
     5401 taaaaagttg cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccggtgtaaa
     5461 acagtactcc tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat
     5521 agtagccagc cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac
     5581 ccgccagttt gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt
     5641 acatggtgtt gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat
     5701 cctgccagcc accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag
     5761 acggaagcat aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa
     5821 acatcaccgt aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc
     5881 atgccagaga aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt
     5941 tatccgcata ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg
     6001 ccagaagcac accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg
     6061 cattgccgat agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt
     6121 cagaacgttt tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa
     6181 agagtccgat ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag
     6241 gagcctgtag cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa
     6301 caccgttcat acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg
     6361 cgagaataaa aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca
     6421 gtcacacatg atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagtcc
     6481 ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt
     6541 cgcggtaaaa tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg
     6601 aattattgac ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt
     6661 tatgccatct gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa
     6721 atcgcaggac acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat
     6781 gcgtaccctg ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg
     6841 gcgcggtaaa cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg
     6901 ggcggaagac ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa
     6961 agcgactgtg cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca
     7021 catcctgcgc gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg
     7081 ccgttccgaa acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga
     7141 aaatggggaa ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg
     7201 gcgtttcttt ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga
     7261 cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgcggacat
     7321 tgtgtcgccg acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc
     7381 gttactgctg ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa
     7441 ctttgccagc catcccatcg gcaccggtcc gtatgcggtg atccgcaaca ccaccaatca
     7501 actgaaaatt caggcattcg atgacttctt cggttaccgg gcattaatcg atgaagttaa
     7561 cgtctgggtt ctgccggaaa ttgccgacga gccagctgga ggtctgatgc taaaaggtcc
     7621 acagggcgag gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt
     7681 cgacagccgc acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct
     7741 ttctccaact aatctgctct atttcgctga ggaacagtac cagcaactgt ggttcccggc
     7801 ttatggactg ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg
     7861 cctggaaagc ctcaccctga ccttttatca ggatcacagt gagcatcggg tgattgccgg
     7921 gatcatgcag cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta
     7981 cgatcagtgg catgaaggag agatcgagag cgatatctgg cttaatagcg ccaactttac
     8041 gctgccgctg gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg
     8101 tattccgatc gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc
     8161 gaactggtgc cagcaactgg tcgccagtaa agcaatggtg ccgcttatcc accactggct
     8221 gatcattcag ggacaacgca gtatgcgcgg cctgcgtatg aataccctcg gctggttcga
     8281 ttttaaatca gcgtggtttg cgccgccgga tccatgattg ctggtagata acaaaatcac
     8341 tacactaacg ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt
     8401 cgaacctgat ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc
     8461 actctttttt gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag
     8521 cgcccgtttt cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact
     8581 gggggcctgg tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc gaactgaaac
     8641 tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata
     8701 gtaaagccga tgtggtgctg gggctggata acaatctgtt agacgccgcc agtaaaaccg
     8761 gactatttgc caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata
     8821 atgacacttt cgtcccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac
     8881 tgaaaaaccc gccgcaaagc ctgaaagaat tggttgagag cgatcaaaac tggcgggtga
     8941 tttatcagga tccgcgcacc agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag
     9001 tctatggcga tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca
     9061 ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga
     9121 gttacaccac ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg
     9181 cgaactttag cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca
     9241 agcagccgga gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg
     9301 cgatcccaac cggtaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg
     9361 aacaattgac caaaccggcc accacgctgg agttcacacc agctgaagtg gctgcacaac
     9421 gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat
     9481 tccaggtgta agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct
     9541 gtggtggaac gcgccgcagg gtaactgggt ggcagtctgg caggacagct acctgtggca
     9601 tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc
     9661 gatattcctc gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg
     9721 tctgtgcgca atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt
     9781 ctatggtcgt cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt
     9841 ttcgccctac ggcctgcaag gcattttgct ggcgcacgta ttttttaatc tgccgatggc
     9901 gagccgctta ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc
     9961 ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg
    10021 acaaatcccg ccggttgccg cgcttatctt tatgctctgt ttcgccagct tcgccaccgt
    10081 gctatcgctg ggcggcggtc cgcaggcaac tactatcgag ctggctattt atcaggcgct
    10141 gagttacgac tacgatcctg cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg
    10201 ccttgggctg gtgctgttga gtcagcgact aagtaaggcc attgcgcccg gtaccacgct
    10261 gctgcaaggc tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt
    10321 aatagtgctg gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatggggt
    10381 aaatcgccag ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc
    10441 gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg
    10501 gagcagtcgc gaactgcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag
    10561 cggcatgttg atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct
    10621 caacaacacc attggcctgc cgcaatctgc tgacggcatt gtgattttca ccaatgcgtt
    10681 aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg
    10741 ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga
    10801 gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat
    10861 tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta
    10921 tctctaccag caaattggct cctatcgcag ccaggacggt gcggtcaccg cgttaattct
    10981 gctactactc tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa
    11041 aactgactga tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag
    11101 aacgcggcga gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga
    11161 atttgatcgc cggttttctg acgccagcca gcggttcgct gactatcgac ggcgttgatc
    11221 acaccacgac gccgccgtca cgccgtccgg tgtcgatgct gtttcaggaa aacaacctgt
    11281 tcagtcacct gacggtcgcg cagaacatcg ggctggggct aaatccggga ttgaaactga
    11341 acgcggcaca gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa
    11401 tggcgcggtt acctggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt
    11461 gtctggtgcg cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg
    11521 cgttacgtca ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc
    11581 tattgatggt gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg
    11641 tcgccgacgg gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga
    11701 gtgcttcggc actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc
    11761 cttatccgac ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat
    11821 caggcaaccc gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat
    11881 acaccggcat caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa
    11941 ttaacggcgt cagccacaac aaacgaccac gggacaaata atgactcaaa cggtcagtcg
    12001 ctttaccact gcgccataac cgccagcaca gccagccacc aacccacaaa aacaccgctg
    12061 ttgccagcag caaccattta aactcaccgc tctgcattcc ggctggaata tcgatcgccg
    12121 cgcccgccag aatccctggc aggaagtaaa acggtggcca cagcaggcag ccgataatat
    12181 tcggcgtaat gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg
    12241 gacgcgtcgg gccaacaaaa cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg
    12301 catgttcagt tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg
    12361 gctttttaaa acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga
    12421 tccctgccag ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc
    12481 ccgccatcag caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg
    12541 ccaccaacac caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca
    12601 atgcttgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
    12661 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
    12721 ttcacttttt cttcacaacc ggctcggaac tcgctcgggc tggccccggt gcatttttta
    12781 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
    12841 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
    12901 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggtgacaag
    12961 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
    13021 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
    13081 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
    13141 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
    13201 gcttcatccg ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca
    13261 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
    13321 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcaa
    13381 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
    13441 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
    13501 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
    13561 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
    13621 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta
    13681 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
    13741 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
    13801 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
    13861 tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat
    13921 tgcaattggc ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag
    13981 cggtgaagag atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg
    14041 tgatgccccg aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc
    14101 ggcactgaaa accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat
    14161 tggcgttgac agtaccggct cgacgcccgc accgattgat gccgacggta acgtgctggc
    14221 gctgcgcccg gagtttgccg aaaacccaaa cgcgatgttc gtattgtgga aagaccacac
    14281 tgcggttgaa gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta
    14341 ctcccgctac attggtggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt
    14401 gactcgccag gacagcgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg
    14461 ggtgccagct ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag
    14521 cgccgggcat aaatctctgt ggcacgaaag ctggggcggc ttgccgccag ccagtttctt
    14581 tgatgagctg gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg
    14641 gactgccgat attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc
    14701 tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg
    14761 cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc
    14821 cgacaaacag agcgttggcg agcgggcagt taaaggtatt tgcggtcagg ttgatggcag
    14881 cgtggtgcct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc
    14941 ctggttcggt cgcgtactca gctggccgct ggaacagctt gccgcccagc atccggaact
    15001 gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc
    15061 caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg gtcgtcgcac
    15121 accgaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc
    15181 tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga
    15241 gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg
    15301 gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt
    15361 tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt
    15421 gcacgcagac atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca
    15481 accgcgcagc gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc
    15541 gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt
    15601 tgcgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca
    15661 ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc
    15721 acgttgttaa tgcgctgaat acggaagcga aactgccctg caaactggtg ttgaaaccgc
    15781 tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt
    15841 gcgctggtct ggtggtgtgg ctgcacactt tctccccggc caaaatgtgg atcaacggcc
    15901 tgaccatgct caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt
    15961 gggacagtat cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt
    16021 tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt cactggcagg
    16081 ataaacaagc ccatgagcgt atcggctcct ggatgcgcca ggcggtctct aaacaggata
    16141 cccgtcatct gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg gtcaccgatg
    16201 gtgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg
    16261 atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt
    16321 acgaaagctg ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg
    16381 tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc
    16441 acgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg
    16501 tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg
    16561 ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg
    16621 aggactacac ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg
    16681 aagtctgccc gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta
    16741 ttggtggtaa ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg
    16801 tcgccagcct gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg
    16861 tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac
    16921 cggatctgcc aactgcttcc gaagcgtgga tccttgctgg tggcgcgcac cataccgtct
    16981 tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa
    17041 tcacggtgat tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg
    17101 aagtgtatta cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac
    17161 gctgacgcgt cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca
    17221 tccggcattg ttttccccct cgcccctttg gggagagggc cggggtgagg ggaaacgacc
    17281 tctcaggagt caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc
    17341 tgccaaaaca caacctggtc acgctcacat ggggcaacgt cagcgccgtt gatcgcgagc
    17401 gcggcgtctt tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata
    17461 tggtcgtggt tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg
    17521 acacgccaac tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata
    17581 cgcactcgcg ccacgccacc atctgggcgc agtcgggtca gtcgattcca gcaatcggca
    17641 ccacccacgc cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag
    17701 aaatcaacgg cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac
    17761 agggtatcga tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat
    17821 ggggcaaaaa tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata
    17881 tggggatatt ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg
    17941 ataaacacta tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata
    18001 aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt
    18061 cagcgtggcg caggcaggtt ttatcttaac ccgacactgg cgggacaccc cgcaagggac
    18121 agaagtctcc ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca
    18181 agagtccgtg gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg
    18241 tgaacaaggc tttcgcctga caccgctggc gttaaaggat tttcaccgcc agccggtgta
    18301 tggcctttac tgtcgcgccc atcgccaatt gatgaattac gaaaagcgcc tgcgtgaagg
    18361 tggcgttacc gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt
    18421 tatcacctca ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg
    18481 tctgaaaccg catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac
    18541 cacccgccac ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta
    18601 tatgctgggg ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc
    18661 cagccgcccg ctgctgctgg aaaaactcaa cgcctggttt gcaaaccacg atcccgatgt
    18721 gatcatcggc tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg
    18781 ttaccgtctt ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg
    18841 ctttaaaaac ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga
    18901 ggcgctgaaa tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga
    18961 gctattaggc gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg
    19021 ccgtttcgcc gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt
    19081 gacgcagatc ttccacaaaa ctgaaatcat gccattttta ctcgaacgag caacggtgaa
    19141 cggcctgccg gtggatcggc acggcggttc ggtggcagcg tttggtcatc tctattttcc
    19201 gcgaatgcat cgcgctggtt atgtcgcgcc taatctcggc gaagtgccac cgcacgccag
    19261 ccctggcggc tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga
    19321 ctataaaagc ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt
    19381 tgaaggcatg gcgcaacctg atccagagca cagtaccgaa ggttttctcg atgcctggtt
    19441 ctcacgggag aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga
    19501 agccaaacgt cagggtaata agccgctttc gcaggcgctg aagatcatta tgaatgcctt
    19561 ttatggcgtg ctcggcacca ccgcctgccg cttcttcgat ccgcgcctgg catcgtcgat
    19621 caccatgcgt ggtcatcaga tcatgcggca gaccaaagca ttgattgaag cgcagggcta
    19681 cgacgttatc tacggagata ccgactcgac ttttgtctgg ctaaaaggtg cacattcgga
    19741 agaagaggcg gcgaaaatcg gtcgtgtact ggtgcagcac gttaacgcct ggtgggcgga
    19801 aacgctgcaa aaacaacggc tgaccagcgc attagaactg gagtatgaaa ctcatttctg
    19861 ccgttttctg atgccaacca ttcgcggagc cgataccggc agtaaaaagc gctacgccgg
    19921 gctgattcag gagggcgata aacagcggat ggtgtttaaa gggctggaaa ccgtgcgcac
    19981 cgactggacg ccgctggccc agcagtttca gcaggagcta tacctgcgca tcttccgcaa
    20041 cgagccatat caggaatatg tacgcgaaac catcgacaaa ctgatggcgg gtgaactgga
    20101 tgcgcgactg gtttaccgta aacgccttcg ccgtccgctg agcgagtatc agcgtaatgt
    20161 gccgcctcat gtacgcgccg ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc
    20221 cttgcaatat cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc
    20281 gctggactac caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc
    20341 cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca
    20401 acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa
    20461 tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac
    20521 ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
    20581 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga
    20641 accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac
    20701 cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatact
    20761 attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg
    20821 ctgacctata tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt
    20881 ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt
    20941 gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc
    21001 cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgattccgca tcagctcaac
    21061 atcgctcatg atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt
    21121 ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct
    21181 gaacgtgtgc taattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg
    21241 cgccgtttca acctgcgctt tgcgctattt gatgatgagc gttatgccga agctcagcac
    21301 gatgcttaca acccgtttga caccgaacag ctggtgattt gctcgctgga ttttgcccgt
    21361 cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat
    21421 gaagcgcatc acctggtgtg gagcgaagat gcgccaagct gtgaatatca ggccattgaa
    21481 caactggcag agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga acagctgggg
    21541 atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg
    21601 cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg
    21661 gcaggtaaca aactgagcaa tgacgaactg aacatgctcg gcgagatgat cggcgagcag
    21721 gatatcgagc cgctgttgca ggcagcaaac agcgacagcg aagatgccca gagcgcccgt
    21781 caggagctgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac
    21841 acgcgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa actgccgcta
    21901 ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg
    21961 gaagatcgtg ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat
    22021 aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc
    22081 catcgctctc agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag
    22141 caggtactgc gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt
    22201 atcgaacgtg accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg
    22261 ctgtgctcag aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg
    22321 tttgacctgc cattcaaccc ggatctactg gagcagcgta ttggtcgtct ggatcgtatc
    22381 ggccaggcgc acgatattca gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg
    22441 ctggtgcgct ggtatcacga aggtctggat gcatttgagc acacctgccc gaccggacgc
    22501 actatttacg atagcgtata caacgatctg attaactatc tggcttcacc ggatcaaacc
    22561 gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacag
    22621 ctggaacagg gtcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag
    22681 gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg
    22741 aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg
    22801 acgccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc
    22861 atcacctttg atcgtgaagt ggcactggcg cgtgaagatg cacagtttat tacctgggag
    22921 catccgctga tccgcaacgg tctggatctg atcctttctg gcgataccgg tagcagcacg
    22981 atttcactgt taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttat
    23041 gtggttgaag cccaggctcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg
    23101 gtacgtatgc tgctggataa aaacggcaac aacctggcgg cgcaggtaga gtttgaaacc
    23161 tttaaccgcc agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg
    23221 cagcaggatg ttcacgctat ccttcaactg ggtgaagcgc agatcgagaa atctgcccgt
    23281 gcattgattg atgcagcgcg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt
    23341 ctggaagctc tgcgtgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag
    23401 agcaaccgtc agcagataat ggaaagcctg gatcaggcag gttggcgtct ggatgccctg
    23461 cgtttgatcg ttgtgacgca tcagtaacgg agctgaaaat ggggatggaa aactacaatc
    23521 caccgcagga accctggttg gttatcctgt atcaggatga ccacattatg gtggtcaaca
    23581 agccgagcgg tttgttgtct gtgccgggtc gtctggaaga gcacaaagac agcgtgatga
    23641 cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca
    23701 gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc
    23761 gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag
    23821 aaggtctggt ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct
    23881 gttacgaaac gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gcggcggata
    23941 acacggcaag agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata
    24001 tgctggcgct ggggcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag
    24061 cgatggcacc gcgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg
    24121 gcaatagtat gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg
    24181 taggcggata aggcgttcac gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg
    24241 cg
//
LOCUS       DAZGXZ010000074        24010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000074 DAZGXZ010000000
VERSION     DAZGXZ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.074"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            54..686
                     /gene="comR"
                     /locus_tag="ACHKJF_003050"
     CDS             54..686
                     /gene="comR"
                     /locus_tag="ACHKJF_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP0950368.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(748..1287)
                     /locus_tag="ACHKJF_003051"
     CDS             complement(748..1287)
                     /locus_tag="ACHKJF_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0950369.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHKJF_003052"
     CDS             complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHKJF_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP0950370.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3226..3768)
                     /gene="ycfP"
                     /locus_tag="ACHKJF_003053"
     CDS             complement(3226..3768)
                     /gene="ycfP"
                     /locus_tag="ACHKJF_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP0950371.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3791..4816)
                     /gene="nagZ"
                     /locus_tag="ACHKJF_003054"
     CDS             complement(3791..4816)
                     /gene="nagZ"
                     /locus_tag="ACHKJF_003054"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP0950372.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAEGVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4827..5651)
                     /gene="thiK"
                     /locus_tag="ACHKJF_003055"
     CDS             complement(4827..5651)
                     /gene="thiK"
                     /locus_tag="ACHKJF_003055"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP0950373.1"
                     /translation="MPFRSNNPITRDELLSRFFPQYHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5632..6273)
                     /gene="lpoB"
                     /locus_tag="ACHKJF_003056"
     CDS             complement(5632..6273)
                     /gene="lpoB"
                     /locus_tag="ACHKJF_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP0950374.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTQVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6287..6664)
                     /locus_tag="ACHKJF_003057"
     CDS             complement(6287..6664)
                     /locus_tag="ACHKJF_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP0950375.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6667..7026)
                     /gene="hinT"
                     /locus_tag="ACHKJF_003058"
     CDS             complement(6667..7026)
                     /gene="hinT"
                     /locus_tag="ACHKJF_003058"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP0950376.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7360..9549
                     /gene="fhuE"
                     /locus_tag="ACHKJF_003059"
                     /pseudo
     CDS             7360..9549
                     /gene="fhuE"
                     /locus_tag="ACHKJF_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(9609..11042)
                     /gene="ptsG"
                     /locus_tag="ACHKJF_003060"
     CDS             complement(9609..11042)
                     /gene="ptsG"
                     /locus_tag="ACHKJF_003060"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP0950377.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11337..12134)
                     /locus_tag="ACHKJF_003061"
     CDS             complement(11337..12134)
                     /locus_tag="ACHKJF_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0950378.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTEG
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12145..13149)
                     /gene="holB"
                     /locus_tag="ACHKJF_003062"
     CDS             complement(12145..13149)
                     /gene="holB"
                     /locus_tag="ACHKJF_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP0950379.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALCLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13146..13787)
                     /gene="tmk"
                     /locus_tag="ACHKJF_003063"
     CDS             complement(13146..13787)
                     /gene="tmk"
                     /locus_tag="ACHKJF_003063"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0950380.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13777..14799)
                     /gene="yceG"
                     /locus_tag="ACHKJF_003064"
     CDS             complement(13777..14799)
                     /gene="yceG"
                     /locus_tag="ACHKJF_003064"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP0950381.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14802..15611)
                     /gene="pabC"
                     /locus_tag="ACHKJF_003065"
     CDS             complement(14802..15611)
                     /gene="pabC"
                     /locus_tag="ACHKJF_003065"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP0950382.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15731..16972)
                     /gene="fabF"
                     /locus_tag="ACHKJF_003066"
     CDS             complement(15731..16972)
                     /gene="fabF"
                     /locus_tag="ACHKJF_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP0950383.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17060..17296)
                     /gene="acpP"
                     /locus_tag="ACHKJF_003067"
     CDS             complement(17060..17296)
                     /gene="acpP"
                     /locus_tag="ACHKJF_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0950384.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17507..18241)
                     /gene="fabG"
                     /locus_tag="ACHKJF_003068"
     CDS             complement(17507..18241)
                     /gene="fabG"
                     /locus_tag="ACHKJF_003068"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP0950385.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18254..19183)
                     /gene="fabD"
                     /locus_tag="ACHKJF_003069"
     CDS             complement(18254..19183)
                     /gene="fabD"
                     /locus_tag="ACHKJF_003069"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP0950386.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19199..20152)
                     /gene="fabH"
                     /locus_tag="ACHKJF_003070"
     CDS             complement(19199..20152)
                     /gene="fabH"
                     /locus_tag="ACHKJF_003070"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP0950387.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20220..21290)
                     /gene="plsX"
                     /locus_tag="ACHKJF_003071"
     CDS             complement(20220..21290)
                     /gene="plsX"
                     /locus_tag="ACHKJF_003071"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP0950388.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21371..21544)
                     /gene="rpmF"
                     /locus_tag="ACHKJF_003072"
     CDS             complement(21371..21544)
                     /gene="rpmF"
                     /locus_tag="ACHKJF_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP0950389.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21596..22117)
                     /gene="yceD"
                     /locus_tag="ACHKJF_003073"
     CDS             complement(21596..22117)
                     /gene="yceD"
                     /locus_tag="ACHKJF_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP0950390.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22316..22900
                     /gene="yceF"
                     /locus_tag="ACHKJF_003074"
     CDS             22316..22900
                     /gene="yceF"
                     /locus_tag="ACHKJF_003074"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP0950391.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23012..23971)
                     /gene="rluC"
                     /locus_tag="ACHKJF_003075"
     CDS             complement(23012..23971)
                     /gene="rluC"
                     /locus_tag="ACHKJF_003075"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP0950392.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         5914 a         6525 c         5789 g         5782 t
ORIGIN      
        1 gtgattattt ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa
       61 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
      121 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
      181 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
      241 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
      301 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
      361 ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact
      421 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
      481 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
      541 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
      601 tcagcgcacg cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc
      661 gtttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga
      721 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt
      781 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
      841 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
      901 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
      961 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cacgaccacc
     1021 accaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg
     1081 attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
     1141 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccccg
     1201 ttcaaacacg ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc
     1261 gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc
     1321 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg
     1381 aaatacttaa ggattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt
     1441 atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc
     1501 cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca
     1561 ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata
     1621 gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt
     1681 tacccatcag gctaccgacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt
     1741 tatactgata atttttcagc ggcttaccgt tcatctgcgc cagtatgttg ttcattgcgc
     1801 aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg
     1861 ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg
     1921 gttccaccac cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt
     1981 ctggcgcttt gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt
     2041 tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc
     2101 caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa
     2161 tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac
     2221 cgtagctgtg cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg
     2281 cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg gcggagtatt
     2341 tcaggaagag attcagcatc tcctggtgaa agcgacgcgc ctggtgcggg ttatcgagga
     2401 aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca
     2461 ttaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcatcgc
     2521 gcagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccagct
     2581 ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat
     2641 caagcgagcc agtcgccact tcgtgcagca gcggtttcca caggtggctg tggttacgat
     2701 cgaccagcgt aattttggct tttttcttac gtcccagctt atgccccagc tgtgttgcca
     2761 tttccagccc accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg
     2821 tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt
     2881 aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat
     2941 tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc
     3001 gggtgagatc aaaaaataga gtttattgtt tattatttga gcaaattcgg aagagtaata
     3061 tagggtgaag cgtatatcga gggcgtttgg tcgtcctgat aagatgcgca gcatcgcatc
     3121 aggcattgat gctcgactgt cggatgcggc gtggacgcct tatccgactt acagaattcg
     3181 gagcgcatca ggtaccggta aggttacctg atgcgacgag ggcatttacc cgagggtttt
     3241 gaacgcttta atgcgctgta aatgcgggga gatattcttg aatttgtgcg tctgctcttc
     3301 gtcccagaca atctcgtaat aatgatgcaa ctcttcagat gtccgctggc tgttaagtgc
     3361 ttcatcatta cgcgacaaaa taaccagaca acgatcgcga ttcttctcgc ggaagttggt
     3421 cacacactta gtggcaatat cggcatactc ttccgggcga tcaatcttgc cttccatgtt
     3481 ctcataaggg aacaaattag ggttgaagat cacctggcgg atatcgcaaa gaaaaccaat
     3541 ccgttccgcc cagtatccgc ccaggccaac gccgcaaatt agcggacgct cgtcaacgtt
     3601 cagttgcaac attttgtcca cttctttaag cagatgctgc atatcatgtt tcggatgccg
     3661 cgtactgtag cttatcaagc gtacatccgg gtcaataaac tgcaattgta agactttctc
     3721 gtggttaccc ggactgttag agtcaaaacc gtgtaaatag ataatcatcg cttcctcaca
     3781 taagccaggg ttagtgacct gctttctctt cctgccagcg ttcgtgtaac tgattcagac
     3841 gggtgctgat cgctttccag cgagccgagt ccatcagttc ctgtcgcgaa aatgaacctt
     3901 tatgatacaa acgtgtaaca ccttctgcct tgatcggtga cagattatct aacacgctta
     3961 cggccccttt acgattattg cagaccagga tcatatcgca acccgcatcc agtgatgcct
     4021 gcccgcgttc ggcataactg cccataatcg cggcaccttc catcgataaa tcgtcagaga
     4081 atatcacgcc gtcaaaaccc agttcctgac gcaaaacggt tttcagccag tagggagaac
     4141 cgctcgccgg acgcggatca acatcactgt agatcacatg cgcaggcata atggcgtcga
     4201 gtttattttc gcggattaag gaactgaaga ccgacatatc tttagcgcga atctccgctt
     4261 gtggacgtgg atcgcacggt gtttctttgt gtgagtctgc cgttactgca ccgtgtcctg
     4321 ggaagtgttt cccggtcgtt ttcattccgg cttcatgcat accatcaata aaccggctgg
     4381 caattgccag ggctttttgt ggatcggcat gataagaacg ctcgccaatc gccgcgctga
     4441 tatgcccgac atccagcact ggcgcaaagc tgatatcaat atccatagcg atcatttcgc
     4501 tggccatcag ccaacccgcc tcttgcgcca gtttgccgcc ctcttccatt cctgacagcg
     4561 cagcgaatga ttgcgccgct ggcaagcggg taaaaccttc acgaaaacgc tgcacgcgtc
     4621 caccttcctg atctaccgcc accaccagat gattgcgcga agctgcgcgg atctggcgca
     4681 ccagttcacg taactgggca ggatcatgat agttacgcgt aaagagaatc agccctccca
     4741 ccagcggatg cgcaagtatt tcacgctctt ccgcgtccag ttcgtaacct ttgacatcca
     4801 acattactgg acccacactg ctctccttat tgttttatta atagctgccg ccaggtgtca
     4861 tcggccagcc tgataaattg ttgatcgccg gtttgtcgcc agcggtactc aaaccaccct
     4921 gctttgagca tcagcagcca gggaaaccat cgcctgacct gacgccataa ttgcgctgga
     4981 taaatcttcg cgcgagtggc atagtcattg accaattgcc ggtgctgttc agtattttcc
     5041 acccacaccg ccgccagttc cagcgcgata tcaccatctc cggcatactc ccagtcgatg
     5101 agttttaacc ctgacgcgct atgcactaaa tttccggcgt ggacatccat atgcaatgga
     5161 cttaagcgta aaggccgtgg ttcccgcgct ttgcgcagac gttttaacat tcgcagccaa
     5221 cccactgtcc gccgcgccgg atcgctttgc tgccagtaca gttccagtaa cggcaacagc
     5281 gttattcgcc agccaaaacg tggttgttga tgtagataat acagcaagcc tgccagttcg
     5341 ttggtatccg gcaaatacgt ttttaccgcg ccgggcagat agtcgactac catccagtca
     5401 cggagatata aatgcggctt cggtgcaatg cttgcgggta gttgtgataa agcccgatac
     5461 tggcgcaaga acgcggactg cggcgcatca ggatcgtgcg gctgacgcac aacaaaacgc
     5521 tggccctgat gttcaatgag aaaactcccg ccactaagcc cactattaaa cgtcgtgacg
     5581 ggatgatact gcgggaaaaa gcgcgacagc aattcgtcgc gcgtgatggg attattgctg
     5641 cgaaacggca cctttacctg accagataat ttcgcccgtc tgcaccagca tcagctgcat
     5701 ttgtagggtc ggagcgttaa cgttgccaga ggcgctggag tacagcacgt aatgagcgcc
     5761 gacattgcgg gcaatgccta tggctttact acgggtgcct aaactgtcct gcggcgacaa
     5821 acctaactgt tgcttagcca tcgacagttg ctgggcggaa acctgggtaa atttcccgtt
     5881 attagccagc gcatttcgca gcgtttcggt cgcttctgcg gcattcagcg aaccgttagt
     5941 acggttgtta acgctatcaa ccagcaggac gctacccgca gtcaccccgt cagccccaag
     6001 catcttactt accatcggct gcattgcgcc attccagtca taatggcgaa tatgcggcgc
     6061 aggcggtgca gtttgatctt cgtgctcaat tgggcctggc tgctgcggga tcgtcggcac
     6121 cgagggcact gtggggacag gctgttgtgg ctcggctggt tgttccggcg ctggtttcac
     6181 ttcttctacc ggtgcaggtt cacgttgccc cacacacccg gcgagaaaca tcgccagcgc
     6241 ggtaatcaag gcgtagcgac tcatttttgt catcaagatt caccccttac aaatatagat
     6301 aaagtctgac tttgtgcgcc cccagaaaat tggcgctgcc gtacagcgtt accgccgaat
     6361 gtgcgggaat gctaacgctg cgtggccttt ccagaggatg catctccagc cctctggcgt
     6421 cataccagta aaaacgataa tgaacggtaa cgggttcttg cctttcgtta taaagcgttg
     6481 aggatgctga aggttgaata tcagacgtcg aaaggacggg cttttctgca ctgatgcccg
     6541 cagccaataa agatgactcc atcaccagcg attgctcatc attcaccgga atttccggat
     6601 gtgaacgaca gcccaccagc agtaacaacg ccagagacac cagcccaaag catccttttc
     6661 tcatcgttac agacctttat gcgccagcat tggtcccagc ggacggccac ccaacaagtg
     6721 catatggatg tggtaaacct cttgtccgcc atggcggttg gtattcatga tcagacgata
     6781 gccatcttcg gcaatacctt cttgctcagc aatttttgcc gctacggtga tcatgcgtcc
     6841 cagcgcctgc tcatgctcag ctgagacgtc gttcacagtc ggaatgagga tattcggaat
     6901 gatcagaata tgcgttggcg cttgcggcga aatatcgcga aacgccgtta ccagatcatc
     6961 ctggtagacg atatcggagg ggatctcacg acgaataatt ttgctgaata tagtttcttc
     7021 tgccacgaca ttttcctttt tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca
     7081 ctgcgagtat agagtatggt cgagttaaac ctgctctttc aaccttaact cacgatttct
     7141 taagcaaaaa atcgttttgc tgccgtatat atcgccatta ttcccatttc tgcctgcgaa
     7201 tgcgctgaaa agacattctc tgtttggttt acacgacaaa tatgaatgcg tatatttctc
     7261 atttgcattt ataaacaaaa ttattcgcac actaaatata atcgctcatc accctcctcc
     7321 ggcatgagcc tgacgacaac ataaaccaag agatttcaaa tgctttcaac acaatttaac
     7381 agggataatc aatatcaagc catcaccaaa ccgtcactac ttgccggttg catagcactg
     7441 gcactattac cttctgccgc ttttgctgca ccagccactg aagaaacggt gattgttgag
     7501 ggttcagcca cagctccaga tgatggcgaa aatgattaca gcgtaacgtc tacctctgcg
     7561 ggtaccaaaa tgcagatgac tcaacgtgat attcctcagt cggtcactat tgttagccag
     7621 cagcggatgg aagatcagca gttacaaacg ctgggcgaag tgatggaaaa cacgctgggg
     7681 atcagcaaaa gtcaggcgga ttccgatcgt gctctttatt attcccgcgg attccagatc
     7741 gataactata tggttgatgg tatccccacc tattttgaat cgcgctggaa tctgggcgac
     7801 gcactttctg atatggcact ctttgaacgc gtagaagtag tgcgtggcgc gacaggactc
     7861 atgaccggga cgggtaatcc atctgcggca attaatatgg ttcgaaaaca cgcgaccagt
     7921 cgtgaattta aaggcgatgt ctcggcggaa tacggtagct ggaacaaaga acggtatgtg
     7981 gcggatttac aaagcccact caccgaagac ggtaaaatcc gcgcgcgaat tgtcggcggc
     8041 taccagaata acgactcatg actggaccgc tacaacagtg aaaagacctt cttctcgggc
     8101 attgtcgatg ctgatttagg cgatcttacg acgctttcag ccggttacga atatcagcgc
     8161 attgatgtta atagccctac caggggcggt ttaccgcgct ggaatactga tggcagcagc
     8221 aacagttacg atcgtgcacg cagtaccgca cctgactggg cgtacaacga taaagagatc
     8281 aacaaggtct ttatgaccct gaagcagcgg tttgctgata cctggcaagc gacactgaat
     8341 gccacccact ctgaagtcga atttgacagc aaaatgatgt atgtcgatgc ctatgtaaac
     8401 aaagcggatg gtatgctggt tgggccatac agtaattatg gacctggctt tgattatgtc
     8461 ggcggcaccg gttggaacag tggcaaacgt aaagttgatg cgctggattt gttcgctgac
     8521 ggtatttatg aattgtttgg tcgtcagcac aatctaatgt ttggtggcag ttacagcaaa
     8581 caaaacaatc gttacttcag ttcatgggcc aacatcttcc cggatgaaat tggcagtttc
     8641 tacaacttta atggcaattt cccacaaacc gactggtcac cacagagcct ggcacaggac
     8701 gataccacac atatgaaatc gttatatgct gccactcgag tcacccttgc cgatccgctg
     8761 catctgatcc tcggcgcacg ttataccaac tggcgggttg atacgctgac ttacagcatg
     8821 gagaaaaacc acaccacgcc ttacgctggt ctggtgtttg acatcaatga caactggtcg
     8881 acctacgcca gctatacctc tattttccag ccgcaaaatg atcgtgacag ttcaggcaaa
     8941 tatctggctc caatcaccgg taacaactac gagctgggtc tgaaatcgga ctggatgaat
     9001 agccgtctga ccaccacgtt agccatcttc cgtattgagc aggataatgt cgctcagtcc
     9061 accggtacac ctatccccgg cagcaacggc gaaaccgcct ataaagcggt ggatgggaca
     9121 gtcagtaaag gggtggaatt tgaactcaac ggcgcaatta ccgacaactg gcagctgaca
     9181 tttggcgcaa cgcgctatat tgcagaggat aacgaaggaa acgccgttaa tcctaatctg
     9241 ccacgcacca cggttaaaat gttcaccagc tatcggttgc ctgtcatgcc agagttgaca
     9301 gtcggcggtg gtgttaactg gcaaaatcgc gtgtataccg acaccgtgac accgtatggc
     9361 accttccgcg ccgagcaagg tagctacgcg ctggtggatc tcttcacccg ctaccaggtg
     9421 acgaaaaact tctcgttaca ggggaacgtc aataacctgt tcgacaaaac ctacgatacc
     9481 aacgtggaag gttctatcgt ctacggcaca ccgcgtaatt tcagcattac cggcacgtat
     9541 caattctgaa taacacctgt aaaaaaggca gccatctggc tgccttagtc tccccaacgt
     9601 cttacggatt agtggttacg gatgtactca tccatctcgg ttttcaggtt atcggattta
     9661 gtaccgaaaa tcgcctgaac accagaacca gcaaccacta cgcccgctgc gcccagtttc
     9721 ttcaggccgg cctgatccac tttagacaca tcagcaacgc tgacgcgcag acgggtaata
     9781 catgcgtcga ggttagtaat gttttcttta ccaccaaatg cagcaaccag agccggtgcc
     9841 atttcgctgg tacctgtcgc ttttgcatct tcagtcgcgt cttcacgacc cggcgttttc
     9901 agatccagtg ctttaatcag cacgcggaag atggtgtagt aaacaatcgc ataaccgata
     9961 ccgacgatcg ggaacagcca cagtttgctg ctgttaccag acagaacgat gaagtcgatc
    10021 agaccgtgcg agaacgacgt accgtcacgc atccccagaa gaatacagat tgggaatgcc
    10081 aggcctgcca gaatcgcgtg gatgatgtac aggatcggcg caacgaacat gaaggagaac
    10141 tcgatcggct cggtgatacc ggtcaggaac gaggtcagcg ccgcggagat cataataccg
    10201 cccactttcg cgcggttttc tggtttagca gagtgccaga tagcaattgc ggcagctggc
    10261 agaccgtaca ttttgaacag gaagccacca gacagtttac ccgcagtcgg gtcacccgcc
    10321 atataacgcg gaatgtcgcc gtggaaaacc tgacctgctg cgttggtgta ttcaccaatc
    10381 tgcatctgga aaggtacgtt ccagatgtgg tgcagaccaa acggtaccag gcaacgttcg
    10441 atgaaaccgt aaatgccaaa cgcaacaacc gggttctggt aagcagccca ctgagagaag
    10501 gtctggattg cagaaccaat cggcggccaa atgaaggaca gcacaacgcc agtaaagatg
    10561 gcagccaggc cagaaatgat cggcacaaag cgtttaccgg caaagaagcc aagatactca
    10621 ggcagcttaa tacggtagaa acggttaaac atgtacgctg cgatcgcacc ggagataatc
    10681 cctccgagta cgccagtatc cgccaggtgt ttagaggcga tttcttcagc aggtaaatgc
    10741 agtaccagtg gcgcaaccac ggccatggtt ttaaccatga tgccataggc aacaactgcg
    10801 gccagcgcgg atacgccatc gttattggta aagccgaggg cgacaccgat cgcaaaaatc
    10861 agtggcatgt ttgcaaagac ggaaccgcct gcttctgcca taacatgcga tacaacggcg
    10921 ggcagccagc tgaaattcgc ggaaccgacg cccagcagaa tacctgcgat aggcagtacg
    10981 gataccggca gcatcagcga tttaccgacc ttttgcaggt tagcaaatgc attcttaaac
    11041 ataattgaga gtgctcctga gtatgggtgc tttttttacg ttctcacgcg tggcaagggg
    11101 ggagagcctc gccgtgtaca gggcatctaa gcgcccttta tttattacac agagtaaaat
    11161 aattcagtgc caatttgttt gacggctatc acgtttcaac ttcagatcat tggagaagtt
    11221 gcacattttg acaaaacata tgtcacaata tttcaaacgt ggttattcac cgcccaattt
    11281 tcggcggtga actttactcg ttttagccat taattacgag ctttaaaaaa actcattcaa
    11341 cggatggatt gaaggcggga agcgtcgatg tgaaacagac gggcgaagtt atcggtggtt
    11401 acctgcgcca gttcttcaac ggcaacacct ttcaacacag ccatgtattc tgcaacgtca
    11461 cgaaccatcg caggttgatt ctcttttcct cgatgcggta ccggcgcaag gtaaggtgag
    11521 tcagtttcca ccagtaatcg atccaggggg acataacgcg cagcatcgcg cagttgctcc
    11581 gcattacgga aggtcacaat gccggaaaag gagatgtaaa atccgagatc cagtaattta
    11641 cccgccgttt ctctgccctc tgtaaaacag tgtagtacgc caccgcaatc cgtcactttt
    11701 tcttcgcgca gaattgccag cgtatcggca cgggcgtcac gggtatggac gataaccggc
    11761 ttgttcagtt cacgaccaat ctggatatga tggatgaagg actcttgctg acgtacttta
    11821 gtttccggtg tgtaataata atccagcccg gtttcaccca gcgctacaac accctcttct
    11881 gccgccagac ggcgtaaatc ttctacatcg tagggatcat tctggttgag tggatgcacg
    11941 ccacaagaaa atacgacgtt gtcacgttcg cctaccagat cccgcatatg taaataaccc
    12001 ggtaatgttg tggcgactgc cagacaaaat ttcacatcgc gtgcggcggc tttcgccaga
    12061 acgtcatcca cgtccttatg caaagattca taatccagac catcgagatg gcagtgtgag
    12121 tcgactaaaa acatgatgtc tctcttaaag atgaggaacc ggtagcacaa cgcccggttg
    12181 caggtaatgc tcaatacgca gtaaaagatc ggtgatgaga agctcgcggt tgatgcctgt
    12241 gacagacatt aactgttcac gaatgtggca aacatccccc agtatagcct gcaggcgcga
    12301 gggagaaaga tggtttgcca gttcggcgac caggcccggc acatcaacat tggtcacctg
    12361 cgcagcgcca tgatggcgtt ttagcgcatc catcagcaac gttgccagcc agtgtaaacg
    12421 cgccggagct tgttcatgat taagggccgc cagcagcgaa taccagtcgc ccgatggcac
    12481 gctatatgcc aacgcctgac acaatgtttc acgagcctgc cagttatctc cctgaaacaa
    12541 cgccagtgcc gcgccaggcg aaccggcgct taagcacaat gcggcaagta atgcatcctg
    12601 tgacattgtc acttcgcgtg aaagccaggt cacggcgtac tgttccggcg gcggcgcaag
    12661 gtaatgtaac cgacaacgac tacgtaatgt tgccagtaaa cgttcaggct cgcgggtagc
    12721 caggaaaaac caggtttctg ctggtggctc ttcaagcgtt ttcagcaatg cgttagccgc
    12781 ggcgtcggtc agtaaggcag catcggttac ccagacaact ttcgcaccac ctaagcgtgc
    12841 gtgctcattc agcttttcgg tgacctcacg taccgcatca atgcccaacg catttttccc
    12901 tttttcggga gccagggtgt agtaatcggg atgcgtgcca gcctgcatca actgacatcc
    12961 acgacagtga ccgcaacttt tgtgaccctg cggttgttgg cagagtaaat aacggctcag
    13021 ggcgtagatt aaagcatcat cgcccatgcc cggtaacgcc tgaatgagca gcgcatggtg
    13081 acctcttccg gcctgatagc tggctaccag tttttcgaaa tcaggtcgta accatggata
    13141 ccatctcatg cgtccaactc cttcacccag tgggtcacgg tagtgcggat tgcatccatc
    13201 acggcctcca gcggctgggt ggcatcaatg gtatgaatgc ttttatcttg tgctgccagt
    13261 tccagatagc gggcgcgggt gcgattaaag aaatcgaaag actcttgctc aatacgatcc
    13321 agctcgccgc gcgcacgcgc gcgttttaag ccaacttccg gggtaacatc gagatagagc
    13381 gttaagtcgg ggcgaaaatc cccgagaaca gcatcacgca gtgttgccag catatgttgg
    13441 tcaataccac gtcctccgcc ctgatacgcc tgagtggaga gatcgtggcg atcgccaatc
    13501 acccaggtgc cgttagccag cgcaggtttg atgactgttt ctaccagttg aacgcgcgcg
    13561 gcataaaaca tcagaacttc ggctttatcg gtaatgactt catcgcctac cgatttgata
    13621 tccagcacca ggcttcttaa cttttcggca agttgcgtac cgccaggttc ccgagtgaaa
    13681 accatgtcgc ggatacccag ttgctcgagc gtctcaacca ccacattacg cgcggtagtt
    13741 ttgcctgcgc cttccagccc ctcaatgacg atatacttac tgcgcatttt tttccttaag
    13801 cactttcaga taatcctgca cagacttgtt atgactggca agattggtat taaacgtgtg
    13861 accaccttta ccatcggcca caaaatagag atacggcgtt tttgccggat gcgcagcagc
    13921 cttcagcgaa tccgcccccg gcgtagctat cgcacccggc ggcaaaccgg taatggtata
    13981 ggtgttatac gctgtcggcg tttccaggtc tgcacgagaa agtttgccat tataacgctc
    14041 tcccatcccg taaatcacgg ttgggtcggt ctgcaggcgc ataccaatgc gtaaacggtt
    14101 gataaatacc gaggcaacct gatcgcgttc actggcaacg gcggtttctt tttcgataat
    14161 tgatgccatc gtcaccagct gatttttatc tttataaggc agaccgtccg cacgcccttc
    14221 ccaggcgcta tcgaccgctt tcaccatttt cttgtgcgct cgcttgagta acgcgacatc
    14281 ggtggtattg gcggtataca tccaggtgtc tggccagaac caaccttcaa tccactccgg
    14341 gttttccagt tcaagtgcct gcgctacggt ggcgtactta tcgtcactca gcgtgtgctt
    14401 gatatacgga gcctcacgca attgcttgag gtagtcgctc agacgcatcc cttctaccag
    14461 tcgcagcggg aactgtgctt ctttaccgct ttccaacaat ttcagcatct cgcgcacggt
    14521 catctgcggt gtaaagcggt aagtcccggc tttaaagtga gaaagatccg gttcgatacg
    14581 cagcagccat tgaaaaaccc gtgggcgatt gatgatctta tcagcataaa gctgttcacc
    14641 gagcgccaga cgtccggtcc ctggcttcag ggtaaatatc gtctcttctt tgataagcaa
    14701 tttgctgtcg gcaagatggc gaaccttcca gacgcccaca ccagcggcga tacccagtac
    14761 caccagcaat aacaagatta tcaataacac ttttttcatg actaattcgg gcgctcacaa
    14821 agtggggcta aatattcata taacgttgct gacgaaaagg agacatcgcc acaggcacgt
    14881 acgggcatca ctggcattaa cgcattacaa ataaccatct catctgcctg caacgcctct
    14941 tccagagaag cttgcacttc gacaagctga taagaggatt gtgccagcaa acggatacag
    15001 aattgtcgca taatgccgtt aacacctgcc tgatccagtc gcggcgtata aactacgttg
    15061 cccttccgcc agaacaaatt agccgcacag cattccgtaa cccacccttc gctgtcaagg
    15121 accagcgcct catcagcgtt tgtctgctca agatgagagc ggatcaatac ttgctcaagc
    15181 cgattaagat gtttaatacc tgcaagatga ggattgcgcc ccagccgcac cgggcttagc
    15241 gccaacgtca tcccttcgtt acgcaaacgg tcgtaatgtg caggataaac cgtaacggag
    15301 agaatccgcg ttgccggtcc actgttcaat gtgctgtacc ctcgcccgcc actaccgcga
    15361 ctgatcacga ctttcagcac accattttgc tgttctgctg ccagcgtttt catctcttgt
    15421 tcaagctgag gccagaagtc acagaaaatc atcaaccgct gacaagcatc ctgtagtcgc
    15481 tggatatgcg ccgataacaa actgacttta ccgtcgataa ctctggcggt ggtaaaacaa
    15541 ccatcaccaa actgcgttgc ccgatcgctt actgccagcg attcctgctt ataaccgtta
    15601 attaagaaca taccggctcc ttatcagaga attgttagtg tggcagcatg ttcactacgg
    15661 aacaagtcgg aataaaagct aagaaaaaag gcccgcaagc ggacctttta tatgggtgga
    15721 aaatgacaac ttagatcttt ttaaagatca aagaaccatt agtgccaccg aagccgaagg
    15781 agttacacag cgtgtattcc attccgctga cctgacgtgc ttcatgcggt acgaaatcca
    15841 gatcgcaacc ttcatccggg ttatccaggt tgatggttgg cggaacagcc tgatcgcgca
    15901 gcgccaggat ggagtagata gattctactg cacccgccgc acctaacagg tgaccggtca
    15961 tagatttcgt ggagcttacc aacacacggc ttgcagcttc accgaagatg gttttcaccg
    16021 cctgcgcttc agctttatcg ccagccggcg tagaagtacc gtgcgcgtta acgtagccaa
    16081 tctgactcgc ttcaatgcct gcatcacgca gagcatttgc cattgccaga gctgcgcctg
    16141 cgccattttc tggcggtgac gtcatatgat aagcatcgct gctcatacca aagccgacga
    16201 gttcagcgta aattttcgca ccgcgttttt tcgcgtgttc gtactcttca agtaccagca
    16261 taccggcacc atcgcctagt acgaagccat cacgctcttt atcccacggg cggctcgccg
    16321 cttgcgggtt atcattgcgg gtagataatg cacgtgccgc gccaaaacca ccaacgccca
    16381 gcggcgtact ggctttctct gcgccacctg caaccatcac gtcagcatcg ccatacgcga
    16441 taatacgcgc agcatgacca atgttgtgca cgccggaagt acaggcggtc gcgatagaga
    16501 tgcttgggcc acgcaggcca tacatgatag tcagatgacc tgccaccatg ttcacaatcg
    16561 ttgacggaac gaagaatggg ctgatcttac gtggaccacc gttcatcaga gatgtgtggt
    16621 tttcttcgat cagtccgagg ccgccaatcc cggagccaat tgcggcacca atgcgggttg
    16681 cgttctcttc cgttatttca aggccagaat cctgcatggc ctgaacgcca gcgacaattc
    16741 catattgaat gaaggcatcc atcttgcgct gttctttgcg cgagataatg tcctcacagt
    16801 taaaatcctt tactaagcca gcaaatttcg ttgcataggc gctagtatcg aaatggtcga
    16861 ttaggctgat gccactctga ccggcaagca gagctttcca ggtagactct acggtattgc
    16921 cgacaggaga caacatgccc agtccggtca caactacacg acgcttagac acgtttgtcc
    16981 tccagggagg gaaaaaatga ttctagtggg acaaaaagat aaaactcagg cggtcgaacg
    17041 accgcctgga gatgttcact tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa
    17101 cggtggtgat tttctcagct tcttcgtccg gaatctcagt atcaaactct tcttccagag
    17161 ccattaccag ctcaacggtg tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat
    17221 tgttggtaac ttcttcctgc ttaacgccca gctgttcgcc gataattttc ttaacgcgtt
    17281 cttcgatagt gctcatactc ttaaatttcc tatcaaaact cgctttcgcg atggttttcg
    17341 tagtgtataa aatgttgaaa aatttgcaac taaatcccgg caggtcttac cacgatttta
    17401 cgttattttg aggccttacc ccccaataac gcaaatcatt ttgcactaat tattactcat
    17461 aaccacgcag actgcgcgca acatgagcat tttgtgcaaa tcgcggtcag accatgtaca
    17521 tcccgccgtt cacatgcaaa gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga
    17581 atgcaaccgc gttggcgatt tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca
    17641 ggatacccgc acgctggtca tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg
    17701 gagcaacaac gtttacagta ataccgcgtg acgcaacttc gcgcgccagt gatttactga
    17761 agccgatcaa gcccgctttc gccgcagcgt agttggcctg accgccattt cccatggtac
    17821 caaccacaga accgatagtg ataatacgac catgacgctt tttcatcata gcgcgcatta
    17881 ccgcttttga cagacggaaa acagatgaaa ggttggtttc gataatatcg ttccactctt
    17941 catctttcat tcgcattaac aggttatcac gagtgatacc ggcattattg accaggatat
    18001 ccacttcacc aaattctgcg cgaatttttt ccagaacaga ttcgatagat gccgggtcgg
    18061 tcacattcaa catcagacct ttgccgttag cacctaaata atcactgatc gcctgagcgc
    18121 cattttcact ggtcgcagtg ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa
    18181 ttgcgcggcc aattccgcgg cttgcaccgg ttaccagtgc gatttttcct tcaaaattca
    18241 tgattttcct cttttaaagc tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg
    18301 aggcggtcag ggtgtcgaca atgcgtttcg tcaggccagt tagcactttg cccgggccga
    18361 cttcatagag atgttctacg ccttgcgctg ccatgtactc aacagacttc gtccactgaa
    18421 ccgggttata caactgacgt accagtgcgt cacgaatggc atcaccattg gtttcgcatt
    18481 tcacatcaac gttattcaca acaggaactg ttggtgcgtt aaaggtgatt ttcgctaatt
    18541 ctactgccag tttgtcggct gctggtttca tcagcgcaca gtgagacggt acgctcactg
    18601 gtaacggcag cgcgcgtttt gcgcccgcag ctttacaggc agcgccagca cgctcaaccg
    18661 cttctttatg accggcaata accacctgtc ccggagagtt aaagtttacc ggagaaacga
    18721 cctgaccttc tgcagcttct tcacacgctt tcgcaataga cgcatcatcc agaccgatga
    18781 ttgccgccat agcgccagtg ccttccggta cggcttcttg catgaactta ccgcgcatct
    18841 caaccagacg caccgcatca gcgaaatcaa tcacgccagc gcaaaccagc gcggagtatt
    18901 cccccaggct gtgaccggcc atcattgccg gtgctttacc gccctgctgc tgccatacgc
    18961 gatacagcgc aacagatgca gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt
    19021 cttcagctgg cccctgctgg gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt
    19081 cagcaaacgt ttcttcgaca attggatagc tcgccgccat atcagccagc attccaacgg
    19141 tttgagaacc ctgtccaggg aacacaaatg caaattgcgt catgttttaa tccttatcct
    19201 agaaacgaac cagcgcggag ccccaggtga atccaccgcc aaaggcttca agcagaacca
    19261 actgccccgg cttaatgcgc ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg
    19321 ccgcagaggt attaccgtgg cgatccagcg tcaccacgac attgtccata gacataccga
    19381 gttttttcgc cgttgcactg ataatacgca ggttagcctg atgcggaacc agccagtcca
    19441 gttgagaacg gtcaagatta tttgccgcca gcgtctcatc aacgatgtgc gccagttccg
    19501 ttaccgcaac cttgaagact tcgttgcccg ccatcgtcag atgaattgaa ttctctggat
    19561 tcacacggtc agcattaggc agcgtcagca actcaccata gctaccgtcg gcatgcagat
    19621 gggtggagat gattcccggc tcttcagagg cagccagcac cgcagcgccc gcgccatcgc
    19681 caaaaataat aatagtccca cgatcggttg gatcgcaggt gcgcgccagt acatcggaac
    19741 cgacgaccag agcatacttc accgccccag atttcacgta ttgatcggct acgcttaatg
    19801 cataggtgaa acctgcgcag gctgctgcaa cgtcaaatgc cgggcaacct ttaatgccca
    19861 gcatgctttg aatctgacaa gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca
    19921 caacgatcag gccaatctgg tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg
    19981 cttcaaagcc catggttgaa acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac
    20041 cggtacgagt gacaatccac tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg
    20101 tccgcacttg ttcgggcaga tagctgccag taccaataat cttcgtatac atgtacgctc
    20161 agtcactttt cggttatata ccgtcacttg caaactgcga gttcgctggc agcgtcctgc
    20221 taccgcagag ttccgctttt gccaccgtcc agcagctcaa aaccagctgg gtatacagat
    20281 tccaggcgag cggcaattcg ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca
    20341 atcgcgaccg caaaagctcg ctgattggct gcaccatgac tttttatcac cgtgccgcgc
    20401 aatcctaaca gacaggcgcc gttatactgg tcggggttga ggtgactgaa tcgcctcgtc
    20461 aggctctttt gtagccaacg ctttaataac agtagccacc acgaccgttt tttcccttca
    20521 ccctgagatt tcagcagaga aaggaacatc ctgacaacac cttccatcgt ctttaacgtg
    20581 acatttcctg taaagccgtc acaaaccagc acatctgtct tgccagttaa caactcattg
    20641 gcttcaagat agccgatata attgatagaa gggattgttt taagcaccgc tgaggcatcc
    20701 cgaatactgt cgagaccctt tacttcttct tcaccaatat tgagcaacgc cacgcgagga
    20761 ttgggaattt ccaccacctc ttcagccagg actgagccca taatggcaaa ttgcaccaac
    20821 attgtgctgt cacaatcgac gttggcccct aaatcgagga ccaccgtttt gcccttttgc
    20881 tgatgtggta ataccgtcac cagcgccgga cgctcaatcc cctccagggg cttgagtaat
    20941 aattttgcca gccccatcag tgccccggta ttaccggcac tgacacaggc ttgcgctcga
    21001 ccttctttca ccagctccag ggccatacgc attgaactcc cacgactggc gcggatagct
    21061 tgcgaaggcc gggcatcact ggcgataact gactgcgcag gaataatctg cagacgcgaa
    21121 cgttgttcaa agtcagcttt agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa
    21181 agaagagtga gttgcgaatt agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa
    21241 gggccaaaat cccctcccat gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg
    21301 ccgcccggtt tggtctttcc ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc
    21361 agacgcgtga ttacttagcg atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt
    21421 ggtgacgcag gtgtttttca ccagaagttt tgtctacaga caggctggtg actgcggtca
    21481 gcgcgtcatg ggaacgacgc atgccacgtt tggaacgggt tggtttattc tgttgtacgg
    21541 ccatggacct tactcctcaa ttacggttta tccccgatcc aacggggagc accaattact
    21601 tacgctttaa gctggctaat acggcaaatg ggtttggctt ttgcgcttct tcaggcagtt
    21661 caccaaagac catgtccgct tcggacactt cacagtgttc agaatcatgc accggaacta
    21721 ccggcaaggc gaggatgatt tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt
    21781 cgttaacctc aatcggttca tacgcttccg gcagtgcttc agcctgttcg tctgaacgca
    21841 caggactaaa acaatacgtt gtgtagacct gatgagtaaa cggcttcccg caacgctgac
    21901 actcgagcgt taccgtcacc ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga
    21961 tagcgaacga catggagcat tccacatcac tgtccacact gactacggat tcggcgacgc
    22021 gctcaacctg atcaggggta tagataccct ggtaatcaag gcgtttttga gccgtacgaa
    22081 ccggatcgag agtcaggggt aattttacct tttgcatagg gcgcgcatat taactttgta
    22141 acgtcataga gtcaaagaaa aaggcagcct gtggttgcct tttgccaata attcgcacac
    22201 attgcgggtt actgctttat tttcgttcag tgctgataac gaacatcaac ctgcaaaacg
    22261 aagcgcatag tttaaaatgg ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc
    22321 taaacttatt ttagcctcca cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat
    22381 ctcttttgaa tgtgcagcac cagaggtcga cgaaacccca cgcagcgacg aatcaccgcg
    22441 acagttggtg cttcgactgg cacaagaaaa agcgcaatct ctggcgtcac gttatccgga
    22501 tcatttaatt attggttcag accaggtatg tgttcttgat ggcgaaatca ccggtaaacc
    22561 gttaacggaa gaaaatgccc gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt
    22621 ctatactgga ctggcgttgt ttaattcggc gaatgggcat ctacaaacag aagtggagcc
    22681 ttttgacgtc catttccgtc acctgagcga ggcggagatt gataattacg tgcgtaagga
    22741 gcatcccctg cactgcgcgg gtagctttaa gagtgaagga tttggcatta cgctgtttga
    22801 gcgtttagag ggacgtgacc ctaacacgct ggttggttta ccgctaatcg cgctgtgtca
    22861 gatgttacgt cgggaaggga aaaacccgct gatgggatag ttgtttgcgc cggatgcggt
    22921 tacgtcgcat ccggcaatcg gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc
    22981 aaccgcccat tatcaggctt atatgcttgt attagcgcgc gttacgcagc ttttgcaaac
    23041 aacgcttcaa accttcatcc atcggcgctt cgatacgcat cacctcaccg gtccccggat
    23101 gggtaaactt caacgctgcg gcgtgcagga acagacgatt taatcccgtg cctgcttcag
    23161 tgagctgtct gtcaaattca cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg
    23221 catactgtgt atgcacacgg atctgatgag tacgtccggt taccgggcta caacgcacca
    23281 gggtggcaaa tgcatagcgt tcttccactt taaagcgtgt ttccgacggt ttgccttcct
    23341 gactgacacg cacgatacgt tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa
    23401 cgctcttcac atgcgactgc cactgaccgc gcaccagcgc caggtaatct ttttgcattc
    23461 ctttttcacg taattgctca tgcagagaac gcaacgccga gcgttttttc gctaccagca
    23521 acacacctga agtgtcccgg tcgagacgat gaaccagttc aaggaaccgc gcttccgggc
    23581 gcaacgcccg caaaccttcg ataacgccga agcttaaacc actgccgcca tgtaccgccg
    23641 taccggaagg tttattcagc accaggatat gatcatcttc atataagatg acgtccgcca
    23701 gcgccgccac cttttgcaga tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa
    23761 ccggtgggat acgcacctca tcacccgctt cgagtttata ttcaggctta atacgttttt
    23821 tgttcacccg cacttcacct ttacgcaaaa tacggtaaat catacttttt ggtacgcctt
    23881 tcaattgggt acgcaaaaag ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa
    23941 caatttttac ggatggagtc tctgttttca tggtgggcga ttctaattag ccaacaggat
    24001 tcgcgccact
//
LOCUS       DAZGXZ010000075        24001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000075 DAZGXZ010000000
VERSION     DAZGXZ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.075"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..92
                     /locus_tag="ACHKJF_003076"
     CDS             <1..92
                     /locus_tag="ACHKJF_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950393.1"
                     /translation="LNTCYIEEVLASCSELGFRTDKMKNLTQI"
     gene            complement(213..980)
                     /gene="yafV"
                     /locus_tag="ACHKJF_003077"
     CDS             complement(213..980)
                     /gene="yafV"
                     /locus_tag="ACHKJF_003077"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP0950394.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            1161..1607
                     /gene="ivy"
                     /locus_tag="ACHKJF_003078"
     CDS             1161..1607
                     /gene="ivy"
                     /locus_tag="ACHKJF_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP0950395.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1650..4094)
                     /gene="fadE"
                     /locus_tag="ACHKJF_003079"
     CDS             complement(1650..4094)
                     /gene="fadE"
                     /locus_tag="ACHKJF_003079"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP0950396.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4334..4912
                     /gene="lpcA"
                     /locus_tag="ACHKJF_003080"
     CDS             4334..4912
                     /gene="lpcA"
                     /locus_tag="ACHKJF_003080"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP0950397.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5118..5885
                     /gene="yafJ"
                     /locus_tag="ACHKJF_003081"
     CDS             5118..5885
                     /gene="yafJ"
                     /locus_tag="ACHKJF_003081"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP0950398.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5856..6596)
                     /gene="dpaA"
                     /locus_tag="ACHKJF_003082"
     CDS             complement(5856..6596)
                     /gene="dpaA"
                     /locus_tag="ACHKJF_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP0950399.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6752..7030)
                     /gene="yafQ"
                     /locus_tag="ACHKJF_003083"
     CDS             complement(6752..7030)
                     /gene="yafQ"
                     /locus_tag="ACHKJF_003083"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFP0950400.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7033..7293)
                     /gene="dinJ"
                     /locus_tag="ACHKJF_003084"
     CDS             complement(7033..7293)
                     /gene="dinJ"
                     /locus_tag="ACHKJF_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP0950401.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7479..8252
                     /gene="yafL"
                     /locus_tag="ACHKJF_003085"
     CDS             7479..8252
                     /gene="yafL"
                     /locus_tag="ACHKJF_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0950402.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(8309..8665)
                     /locus_tag="ACHKJF_003086"
     CDS             complement(8309..8665)
                     /locus_tag="ACHKJF_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0950403.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAHM"
     gene            complement(8658..8936)
                     /locus_tag="ACHKJF_003087"
     CDS             complement(8658..8936)
                     /locus_tag="ACHKJF_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP0950404.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(9041..10753)
                     /locus_tag="ACHKJF_003088"
                     /pseudo
     CDS             complement(9041..10753)
                     /locus_tag="ACHKJF_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            10725..11510
                     /gene="lafU"
                     /locus_tag="ACHKJF_003089"
                     /pseudo
     CDS             10725..11510
                     /gene="lafU"
                     /locus_tag="ACHKJF_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
     gene            11581..12636
                     /gene="dinB"
                     /locus_tag="ACHKJF_003090"
     CDS             11581..12636
                     /gene="dinB"
                     /locus_tag="ACHKJF_003090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP0950405.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            12633..13085
                     /gene="yafP"
                     /locus_tag="ACHKJF_003091"
     CDS             12633..13085
                     /gene="yafP"
                     /locus_tag="ACHKJF_003091"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0950406.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            13331..14470
                     /locus_tag="ACHKJF_003092"
     CDS             13331..14470
                     /locus_tag="ACHKJF_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP0950407.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALIGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            14467..15081
                     /gene="prfH"
                     /locus_tag="ACHKJF_003093"
     CDS             14467..15081
                     /gene="prfH"
                     /locus_tag="ACHKJF_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0950408.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(15138..16595)
                     /gene="pepD"
                     /locus_tag="ACHKJF_003094"
     CDS             complement(15138..16595)
                     /gene="pepD"
                     /locus_tag="ACHKJF_003094"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0950409.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            16856..17314
                     /gene="gpt"
                     /locus_tag="ACHKJF_003095"
     CDS             16856..17314
                     /gene="gpt"
                     /locus_tag="ACHKJF_003095"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0950410.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            17406..18650
                     /gene="frsA"
                     /locus_tag="ACHKJF_003096"
     CDS             17406..18650
                     /gene="frsA"
                     /locus_tag="ACHKJF_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0950411.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            18708..19109
                     /gene="crl"
                     /locus_tag="ACHKJF_003097"
     CDS             18708..19109
                     /gene="crl"
                     /locus_tag="ACHKJF_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0950412.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(19148..20203)
                     /gene="phoE"
                     /locus_tag="ACHKJF_003098"
     CDS             complement(19148..20203)
                     /gene="phoE"
                     /locus_tag="ACHKJF_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0950413.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            20491..21594
                     /gene="proB"
                     /locus_tag="ACHKJF_003099"
     CDS             20491..21594
                     /gene="proB"
                     /locus_tag="ACHKJF_003099"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP0950414.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            21606..22859
                     /gene="proA"
                     /locus_tag="ACHKJF_003100"
     CDS             21606..22859
                     /gene="proA"
                     /locus_tag="ACHKJF_003100"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP0950415.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            22974..23049
                     /locus_tag="ACHKJF_003101"
     tRNA            22974..23049
                     /locus_tag="ACHKJF_003101"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23007..23009,aa:Thr,seq:cgt)
     gene            23657..23815
                     /locus_tag="ACHKJF_003102"
     CDS             23657..23815
                     /locus_tag="ACHKJF_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950416.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         5887 a         6086 c         6159 g         5869 t
ORIGIN      
        1 cgttaaacac atgttatata gaagaagtgc ttgcatcctg ctcagagctt gggtttcgaa
       61 ctgacaaaat gaaaaattta acacaaattt aattcatgct cttgccctgc ggggggatta
      121 ttaaatcttc ccagtttttt attatttaac tctcgctcag cacactaaac atccctttct
      181 gaattccccg ccctaaatac agcccccact cactacaacc taaactcatc cgcatcccgc
      241 catgccggaa acttatcgcg atattccctt aacaccgcca tcgacagctc ggcatcaatg
      301 cgcgttgcct gatgcgcgtc ggcagtagcg ataatctcgc cttgtggatt aatcacccgg
      361 ctgtcaccgc gataatggca gccgttgcca tcgctgccga cgcgattgca tcccgccaca
      421 tacgcctgat tctcaatcgc gcgggccgtc agcaatgcct gccagtgcag agagcgcgga
      481 gcaggccagt tggcgacgta cagggcgagg tcataatcgt tgagattgcg cgaccacaca
      541 ggaaaacgta agtcgtagca caccagcggc aaaatacgcc agccgcgcca ttccacaatc
      601 actcgcgcat tgcccgcttt ataatgtaga tgctcatctg ccatgcggaa cagatgacgc
      661 ttatcataaa aatgtaccgt gccgcccggc tcaaccagca aaaagcggtt aaccgaacca
      721 gactccgttt gtaatgcaac actgcctgca atcagcgcat tgcactgctg cgccttagct
      781 gtcatccagt tcactacgtc atcttgtgct agcgacgaag ctgccgcttc catggcaaag
      841 ccgctggtaa acatctccgg tagaacgatc acatcgcgcc cggtaatacc ttccagttga
      901 cgatcaaaat gacgcaggtt ggcagggcca tccatccaca ccagtggttg ctgcaaaagc
      961 gtaatcttca aaccaggcac ggtgtacaac tcctttatgc gaagggtttt ataactttaa
     1021 caccttatca ggcagttgcc ttagcgcaga ataaattgat aacaaatgct gatattggaa
     1081 atatctgatt tgcaaattat cgtgttatcg ccaggcttta gaaggttaat aacatgggca
     1141 ggataagctc gggaggaatg atgtttaagg caataacgac agtcgccgct ctggtcatcg
     1201 ccaccagtgc aatggcgcag gatgatttaa ccattagcag ccttgcaaag ggcgaaacca
     1261 ccaaagctgc atttaatcag atggtacaag ggcataagct gcctgcctgg gtgatgaaag
     1321 gcggtactta tactcccgca caaactgtaa cgttgggaga tgagacgtat caggtgatga
     1381 gcgcgtgcaa accgcatgac tgtggctcgc aacgtatcgc tgtgatgtgg tccgagaaat
     1441 ctaatcagat gacggggctg ttctcgacta ttgatgagaa aacgtcgcaa gagaaactca
     1501 cctggctgaa tgtgaacgat gcgctttcga ttgatggtaa aacggtgctg ttcgcggcgt
     1561 tgaccggcag cctggaaaac catccggatg gctttaattt taaataatta gcggataaag
     1621 aaacggagcc tttcggctcc gttattcaat tacgcggctt caactttccg cactttctcc
     1681 ggcaacttta ccggcttcgt cgccagctct tccggatcaa agtcatcaac gttaatactg
     1741 cgcagacggc tttcttcagc tttcaccaga atagcggctt catctttatc aatcagcccc
     1801 ttcgccagcg cgttgtgcgc cagttcatcc agacgggtaa acggcaggtt tttacccagc
     1861 tctttacaga tccgctgatg aattgggtcg gcggcaatca catccaccag cgcctcttcc
     1921 agcaagccaa ccggattatg ctcgctcggc gtcaggtact gaccgcgacc aatgcgggaa
     1981 cgggtggcgt tcggcacttg taaaatcttc gccactttat gatccagctt gtcagaaggt
     2041 gccagataat gacgtccggt cgggaagatc accacattca gcagcccggc aaccacgcgg
     2101 ttcgggaagt tttgcagtaa atcatccatc gcctgttcag cctgatacag cgcatcttgt
     2161 acgccccagt gcaccagcgg caggtcggct tcattacggc cttcgtcgtc ataacgcttc
     2221 agaacggcag aggcgaggta aagctggctt aaaatatccc ccagacgtgc cgagatgcgc
     2281 tcacgacgtt tcaggctgcc gcccagcaca gccatcgaga catcagaaag cagggcgagg
     2341 ttggcgctca ggcggttcag gtgctgatag tagcgtttag tggcatcgcc ggttggcgtg
     2401 ctgctggtta aaccgcgcgt caggcccagc cagaagctgc gaactttgtt gctaccgacg
     2461 tgaccgatat gtttgaacaa cagtttatcg aacgcgttga cgtcattgtt cttcgctgct
     2521 tccatctctt ccagcacgta cggatggcaa cgaatcgctc cttgtccgaa gatcatcatg
     2581 ctgcgggtca gaatgttggc cccttcaacg gtgatggcaa tcggtgcgcc ctggtaagca
     2641 cgcgccagga agttgctctg cccgagcata atgcctttgc caccagtgat atccatcgca
     2701 tcaataatcg actgctgccc gcggtgggta cagtgatact taacgatagc cgacagcacg
     2761 gcaggttttt cgccgagcat aatgccgtag gtaatcagcg atgccgcagc atccatcacg
     2821 taggcattac cggcaatacg cgccagcggc tcttcaatcc cttccatctt accgatagag
     2881 attttgaact gacggcgaat gtgagcatac gcgcccgttg ccagcgctac tgatttcacg
     2941 ccgccggttg agttggaagg cagggtgatg ccgcggccta ccgagaggca ctccaccagc
     3001 atccgccagc cttgcccggc cattttcggc ccgccgataa tgtaatcgat cggtacgaag
     3061 acatctttac cgcgcgtcgg tccattctgg aacggcacgt tcagcgggaa gtggcgacga
     3121 ccaatttcca cgcccggcgt ggtggttggg atcagtgcac aggtaatacc taaatcttct
     3181 gcgccaccca gcaatttctc cgggtcggag agtttaaacg ccagcccaag cacggtcgca
     3241 atcggtgcca gcgtaatgta gcgtttgttc caggtcagac gcatccccag cacctgctgg
     3301 ccctgccatt cgcccatgca gacaatcccg gtatccggga tagcgcctgc atcggaaccc
     3361 gcttccgggc tggtcagtgc aaagcaaggg atctcctgac cacgcgccag acgcggcaga
     3421 tagtgatttt tctgctcgtc agtgccgtaa tgttgcagca gctcgcccgg gcctaatgag
     3481 ttcggcacgc cgacggtaat cgccaggatc ccgctcacgc cggagagttt ttgcagcacg
     3541 cgagactggg cataagccga gaactccagc ccgccgtact cttttttgat gatcatcgcg
     3601 aagaaacgat gctctttaag gtacgcccac aactccggcg gcaaatcagc cagctcatgg
     3661 gtgatctgga aatcattcgc catccggcag gcttcttcta ccggaccgtc gagaaacgct
     3721 tgctcttctg cggtcaggcg cggctgcgga tagttatgca gctttttcca gtccggcttg
     3781 ccctggaaca agtcgccctc ccaccaggtg gtgcccgcat caatcgcttc tttctcagtg
     3841 cgcgacatcg gcggcatcac cttacggaaa ccgcgaaata ccggcgcgga aatcatcgac
     3901 ttacgcatag gcgcaaagtt aaatggcacg aggataatgg ccagaggcac cagtacccac
     3961 gccgaccaca gaccagcaac gccgagggcg gctgtccagg cgagcaaaat cagactgctg
     4021 ataaataagc tcacgcggtg atagaacaac gcgccgagca ggacaaccgt agcgagaata
     4081 ctcaaaatca tcataacgaa aagcccctta cttgtaggag gtctgaccac ttgtgatgat
     4141 atggttgtag tggatgtaaa aatatttagc aatgtgttta caatataatt acaacaaagc
     4201 tcacattgtt gctgttttta tccgcacttc aggtcaaaaa gtcctggtca tagcacctgc
     4261 ccgtacttct cgcttttggc ggtatccggt acactgcatt ttgtctatta catttatgct
     4321 gaaggatatc ctcatgtacc aggatcttat tcgtaacgaa ctgaacgaag cggcggaaac
     4381 gctggctaac tttttaaaag atgacgccaa tattcacgcc attcagcgcg cggcggtcct
     4441 gttagcagac agctttaaag ccggtggcaa agtgctttcc tgcggcaacg gcggttccca
     4501 ttgcgacgct atgcactttg ccgaagagtt gaccggtcgc taccgtgaaa accgtccggg
     4561 ctacccggcg attgctattt ctgacgttag tcatatttca tgcgtcggta acgatttcgg
     4621 tttcaatgat attttctccc gttacgttga agcggtaggt cgcgaaggcg atgtactgct
     4681 ggggatctcc acctccggta actctgcaaa cgtgatcaaa gcgatcgcag cggcgcgtga
     4741 gaagggaatg aaagtgatca ccctgaccgg taaagacggc ggcaaaatgg ctggcacggc
     4801 ggatatcgaa attcgcgtac cgcactttgg ttatgccgac cgcattcagg agattcacat
     4861 taaagtgatc catatcctga tccagttgat tgaaaaagag atggttaagt aagtctggcg
     4921 taggccggat aagacgttta cgccgcatcc ggcaattgtg caccgatgcc tgatgcgacg
     4981 ctgacgcgtc ttatcatgcc tacaaatctg tacgcgaacc gtaggccgaa taatgcgttc
     5041 acgccgcatc cgacctgaaa attcttaaat caatcttcgc cgggggccat gcgctcccgc
     5101 tgttgtggag gttacccatg tgcgaattgc tcgggatgag cgccaacgtc cctaccgata
     5161 tctgctttag tttcaccggg cttgtacagc gtggtggtgg aaccgggcca cataaagatg
     5221 gctggggcat taccttttac gaaggtaaag gctgtcgcac atttaaagat ccacaaccca
     5281 gctttaattc ccccatcgcc aaacttgtcc aggactaccc gataaaatcc tgttcggtgg
     5341 tggctcatat tcgccaggct aatcggggcg aggtggcgct ggaaaatact cacccattta
     5401 cccgcgagtt atgggggcgt aactggactt atgcccataa cggacaactg acgggctaca
     5461 aatcactgga aaccggcaac ttccgcccgg tcggtgaaac cgatagcgaa aaagccttct
     5521 gctggctcct gcataaatta acgcagcgtt acccgcgcac accgggcaac atggcggcag
     5581 tgtttaaata tatcgcctca ctggcggatg aactgcggca gaagggcgtt ttcaacatgc
     5641 tgctttctga cgggcgctat gtgatggcgt attgctcgac taatttacac tggatcaccc
     5701 gccgcgcgcc gtttggcgtg gcaacgctgc tggatcagga tgtggaaatc gacttcagct
     5761 cgcagaccac accgaatgat gtggtcacgg tgattgcaac acagccgctg acgggcaatg
     5821 aaacctggca aaagattatg ccaggcgaat ggcgcttatt ttgcctcggg gagcgtgtag
     5881 tttgatgcca gttgtggctg cacaacttcg tggcttaacg gcttgctgac cacgtaacga
     5941 ccattgacca cagaaacggt cggtggctta cgggtttgct caaagtagtc gtaacccggc
     6001 ttcagttgct cccagaagtc cttaaagttg gaatatttat ggcgcttcat attggcgtcg
     6061 gtcatgcgga acgggtaaat actcacctgc acgctcggct gaccaaacac cagcgcacca
     6121 gtaacgaact ggaagatctc atcaataccc tgattggtca ttgcgtagca gccgatggaa
     6181 acacaatcgc cgtggatcat caggtatttc ccttcataac catgcgcacg gtcataggca
     6241 ttggggaaac caatattaat cgctttgtag taacggctgt ctggttttaa ctgattacgc
     6301 tggacgctat aaaacccttc cgggcttttg aaatcgccct gacgctgttt tggccctaag
     6361 ccgccggaat atttacagat tttataactg tcgagcagct gatattgctc gcccattttg
     6421 acgtagagat cgagcgtacg ttcttccttg aagatctgga tgtagacagg ggatcccatc
     6481 aactgctgct tatactcttt gctcactggc gtggtcgagc tactgctgcc cagcaaaccg
     6541 gcaaacgaaa cgcacgggat caacaacatc gcaagaatta atgcgatttt acgcatactg
     6601 cttattcctt gataaaacgg ttacacacgc caggacggca aaatagatcc caaatcggaa
     6661 tagtctggat ttggaaggct cacattatca ccaaaagagt tttacgcaag cctgtcggcg
     6721 cagggtttac aaatttcatc ggcagcgtgc tttacccaaa gagcgccgcg tgagttccag
     6781 ttctctcaaa tcgtaaaagt ttatcggtaa gtttgtaaat caggatccag tccggttcga
     6841 catgagcatc gcgataacct ttccatgaac cttgcagcgg gtggtcttta taaacagctg
     6901 gaagcggtaa agcattattg ataagaagcg tcataagata tttcaattta ttcatatcct
     6961 tatgacgctt ttgtgcaagt tttacatcct ttgaaaattg tcccgagtat tcaatatccc
     7021 tttgaatcat atttaaattc ctaatttatc aaataaatca tcggcgtctt tggccttatg
     7081 aacatcaacg ccagcttcgc tgtttttgat tgattgaatg gttaattgat taggctcgcg
     7141 taaatcaaac ggcaatgcct tttcacgcgc gacctttgtg agggttatgc gaaccaggtc
     7201 agagatggtc agccccatcc cggccagtac gtccgccgcc tgattcttca gatcttcatc
     7261 gattcgggcg cgaacaaatg cgttagcagc cattggggta tctcctgtgc tgtatattta
     7321 ttcagcttga attgtagctc aaatgagcaa cagcgacaat ccatgagtaa tttaataaac
     7381 ggatgttttt acataattaa ctgaaatata tttattaatt ttataataat ggtttattaa
     7441 gtttgttcag gtacgggccg ggatacactg caacatcaat gcatgatcag ccattattga
     7501 gtatgtcctt gccgtcgatt ccctcttttg tgttgtcggg attactgttg ctttgcttgc
     7561 cgttttcttc atttgccagc gccaccacat cacatatcac tttcagctac gccgcccgcc
     7621 agcggatgca aaatcgtgcg cgtttattaa aacagtacca aacccatctg aaaaaacagg
     7681 ccagctatat tgtggaaggc aatgccgaaa gcagaagggc gctacgccag cacaaccgcg
     7741 aacagataaa acagcatcca gaatggtttc ctgcgccgct caaggcgagt gacagacgct
     7801 ggcaggcgct ggcggaaaac aaccatttct taagcagcga ccatctgcat aacatcaccg
     7861 aagtagcgat tcaccgtctg gagcaacagc ttggcaagcc ttacgtatgg ggcggcacgc
     7921 ggcccgatca aggcttcgac tgtagcgggt tggttttcta tgcctacaac aagatccttg
     7981 aggcgaagct cccacgcacg gccaacgaga tgtatcacta ccatcgggca acgattgtgg
     8041 cgaataacga cctgcgccgg ggagatttgc tgtttttcca tatccacagc cgcgagattg
     8101 ccgatcatat gggggtgtat ctgggcgatg gccagtttat cgagtcgccg cgcaccgggg
     8161 aaaccattcg ggtaagccgg ttagccgaac ctttctggca ggaccatttt ttgggcgcgc
     8221 gcaggatttt gacggaagag acgattttgt aaatgggtaa tattgacgaa ctggcaaaag
     8281 gccggatgcc acgcggcatc cgacttgttt acatatgggc tgaaaactca cgttctaaga
     8341 catcaacaat gagctggttt tttgaaatgg aatgttgtct ggcgacgatg gtcagccgcg
     8401 aatcaatgtg atgaggaaca cgaagcgtca ggcgtttttg ctcttcttct tcacgaaaag
     8461 gggcaatacc gtcctcttca caatccgcca tccactcatc cagcgcgctt tcgccctcac
     8521 gatataatgc ctcaatgctt gtggctacaa agtcacaata accgtttaca ttgaggaact
     8581 tgccgcgaaa tgcgttgata tcggcttcaa atgtcactgc ggctggttga cctttaattg
     8641 tgataatgtt cgatgcttta ttcataatct acccctacac agttaacaaa ccactcctta
     8701 agacctgcta ctgcaccttt atccatcaca ttctttggat gtggctcatg tgtactgaat
     8761 ttggtcgttc caatctgaaa tttccttctg gacccattgt tattttttat tgttccaccc
     8821 accgctttaa tgagcgcaac aacatcatcc catttaattc cagattttgg cggctccgtc
     8881 attacatcgt cgaatgtttt tctatggcgg gagcctagcg cgctgacctt aatcatacta
     8941 tcaatccatg acgtcattgt atggtgtcat cgtagtatgt ttgcaatgtg acgtcaatca
     9001 atggtgtcag atgcgtgcaa agcatggaaa agaatcctca tcaactcaac gctcccataa
     9061 tcttcaactc cagctcatcc ggcacttcat tatacgacag cacatgcagc cccggcgcaa
     9121 acaacctcgc atagcgtgcc agcaaagggc gcaactgcgg cggcaccagc agcaccgggt
     9181 ctttccccgc cgctttcatc tgctccttca cctgcggcat cgtactctgg aactggttga
     9241 gcatattcgg gtcgaccggc acgctgtcga gcatcacttt gccgccctgt tgcgcctgat
     9301 tcaccacgtt ggtcagcaga ttttccagtt cattattcag cgtatatacc gtcagctcct
     9361 gcttgtgaac gaacggatgg gtaatgctgc gccgcaatgc caggcgcaca tcggccgcca
     9421 gcagaatatg atctttggtc accgcgctac tggcgaccag cacggtggcg atggtgacga
     9481 tatcgcgcag ggaaacgcct tcggtcagca acgcccggta cactttcagc aactggctgt
     9541 aattgagcgc cgcgcttaag tcttccgcca ggcgcggggc catcgacgac aggcggttat
     9601 gcagttgcgt aatgtcatca tagttaaaca aatcaggaat atagctgcgc acaatcttgt
     9661 ttacatgggt ggcaattacg ctggcgctgt cgatcacctg ataacccata ttcagcgcct
     9721 tcgctttctg cgcaggctgg atccaggtca ccggcatccc gtacgccggg tcgttcccca
     9781 atacgccgtc aatctcgccg taggtttcgc tggagggcag ggccatcagt ttatccgccg
     9841 gaatatccgc ttcatcagcc tttatgccgt tgatgaaaat ggcgtactgg ctgggcttga
     9901 ggcggaagtt ttcccgaatg cggatctccg gcaacaatac gccgttgccg tcagaaatca
     9961 cctgccgcac gccgcgaatc cgctgggtga gcgggttacc ctgcgcttta tcgaccagtg
    10021 ccaccagctt gtagccgagg cttaaactga taggctcgat cagcggtatt gtctcccaac
    10081 tgacttgttg ctcgctggtt tcagtgatgg tgcgggtcag cgtttcgagg cttttctctt
    10141 ccgcctctgc cgcctgcggg cgtttgctca tccgccagcc ggtaaagcca agcagggcgc
    10201 tgaacagcaa aaacggcaga tgcggcatcc ccggcaccac cgccagcaca aacataattc
    10261 cggcggcggt gtagagcacc gacgggcttg ccagcagctg gttacgcaca tcatgggcga
    10321 tatcgccgtt gtcgctgacg cgggtgacga taattgccgc cgcggtggag agcagcaggg
    10381 aagggatctg cgccaccagg ccgtcgccga tggtcatcag cacatactgc tggaaggcag
    10441 catcggcgct caggttgtat ttgaagatcc cgatacagac gccgccgatc aggttgatcg
    10501 ccagaatcat catcccggcg atggcgtccc cgcgcacaaa cttcgatgcc ccgtccatcg
    10561 cgccgtagaa atcggcctcg ctggcaacat ctttacgccg ggtttgtgcc tgtgcctggt
    10621 tgatcaaccc ggcgttaaga tcggcatcaa tcgccatctg tttaccgggc atcgcgtcta
    10681 aggtaaagcg ggcagaaacc tcggaaatac gctcggcccc tttggtgacg acgataaagt
    10741 tgattgtcaa cagcgtcagt aaatccgagc gtgaaagcat tatcgccgcg ctgcacgggc
    10801 agtcgatttt taacggtggt gggctgtcgc cgttaaataa aatcagcccg tctcatccgc
    10861 caaaacctgc caccgtggcg gtgccggaag agacggagaa aaaagcgcgt gacgtcaatg
    10921 agaaaacggt gctgctgaag aagaaatccg ccaccgagct tggcgagctg gcaaccagta
    10981 tcaacaccat tgcccgtgat gcgcatatgg aagcgaatct ggagatggag attgtcccgc
    11041 agggataacg cgtgctgatt aaagacgacc agaatcgcaa tatgttcgaa cgcggcagcg
    11101 cgcagattat gccgttcttt aaaaggctgc tggtggagct cgcgccagtt ttcgactcgc
    11161 tcgataacaa aattattatt accgggcata ccgatgcgat ggcctacaaa aacaatatct
    11221 acaacaactg gaacctttcg ggcgatcgcg cgctttcagc tcgtcgggtg ctggaagagg
    11281 ctggaatgcc ggaagataaa gtgatgcagg taagcgcaat ggcggaccag atgctgctgg
    11341 acgcgaaaaa tccgcaaagc gcgggcaacc ggcgcattga gattatggtg ctgaccaaaa
    11401 gtgcgtccga tacgctgtat caatactttg gtcagcatgg ggataaagtg gtgcagccgc
    11461 tggtgcaaaa gctggataag cagcaggtgc tttcacagcg aacgcgctag atgctgaatc
    11521 tttacgcatt tctcaaaccc tgaaatcact gtatacttta ccagtattga gaggtgagca
    11581 atgcgtaaaa tcattcatgt ggatatggac tgctttttcg cagcggtgga gatgcgcgac
    11641 aatcccgccc tgcgcgatat ccctattgct attggcggca gccgcgaacg tcggggggtg
    11701 atcagcaccg ccaattatcc cgcgcgtaaa tttggcgtac gtagcgctat gccgacaggg
    11761 atggcgctca aattatgccc gcatctcacc ttgcttccgg ggcgctttga cgcctacaaa
    11821 gaagcctcaa atcatattcg cgaaattttc tcgcgctaca cctcgcgcat tgaaccgttg
    11881 tcactggatg aagcctatct cgatgtcacc gatagcgtcc attgccacgg ttctgcgact
    11941 ctcatcgccc aggaaatccg ccagacgatt ttcaacgagc tgcaactgac ggcatctgcg
    12001 ggcgtggcac cagtaaagtt tctcgccaaa atcgcctccg acatgaataa acccaacggt
    12061 cagtttgtga ttacgccggc agaagttccg gcatttttac aaaccttacc actggcaaaa
    12121 atccccggcg tcggcaaagt ctcggcggca aaactggaag cgatggggct acgaacctgc
    12181 ggtgatgtac aaaagtgtga tctggtgatg ctgcttaaac gctttggcaa atttggccgc
    12241 attttgtggg agcgtagtca ggggattgac gagcgcgacg ttaacagcga acggttgcga
    12301 aaatccgtcg gcgtggaacg cacgatggcg gaagatatcc accactggtc tgaatgtgaa
    12361 gcgattatcg agcggctgta tccggaactt gaacgccgtc tggcaaaggt gaaacctgat
    12421 ttactgattg ctcgccaggg ggtgaaatta aagtttgatg attttcagca aaccactcag
    12481 gagcacgtct ggccgcggct gaataaagct gacttaatcg ccaccgcgcg taaaacctgg
    12541 gatgaacgcc gcggcgggcg cggtgtgcga ctggtggggc tgcatgtgac gttgcttgac
    12601 ccgcaaatgg aaagacaact ggtgctggga ttatgaataa catacaaata agagactatc
    12661 agcctggcga ttttcagcaa ctatgcgcta ttttcattag agcggttacg atgactgcca
    12721 gtcagcatta ttcaccacaa caaattgccg cctgggcgca gattgacgaa tctcgctgga
    12781 aggagaaact cgcgaaatca caagtgcggg ttgcagtcat taatgcaaaa ctggtcggct
    12841 ttattacctg cgttgaacac tatatcgata tgttatttgt tgaccctgaa tacacccgcc
    12901 gtggggttgc cagcgctttg ttaaaacctt tgattaagtc tgaatccgaa cttacggtgg
    12961 acgcaagcat aaccgcaaaa cccttttttg aacgttatgg ttttcagaca gttaagcagc
    13021 aacgcgttga atgtcgggga gagtggttta ttaattttta tatgcgttat aaacagcaac
    13081 attaaatcca gcttgcaatg aaaataacgc aagcctagta tgtgcgcgtt ttcttattcc
    13141 acaaactgca aggaggtaaa tcatgacaaa tcctttatca atgactcttt gcagaccttt
    13201 ccaggattaa ttcttttttt cttgccctgg attcgtctgc catttcctga ttttttaatt
    13261 gttactctca ataattcaag tcgcaacaca tgcaacttga agtatgacga gtctatttac
    13321 ggaatggtat atgggcaatt atattcgtcc cttatccgat gcggtattta ccatcgcatc
    13381 tgatgacctg tggatcgaga gtttagcgat ccaacaatta cacaccacgg caaatttacc
    13441 caacatgcag cgcgtagtgg ggatgccaga tttacatccc ggacgcggct atccgattgg
    13501 cgcagcgttc ttctctgttg gtcgttttta tccggcactg atcggcaatg atatcggctg
    13561 cggtatggcg ctatggcaaa cagatattct cactcgcaaa tacaacgccg ataagtttga
    13621 aaagcgatta tcagatctgg atgacgttgc tgaagaaagc tggctggagg aaaacctgcc
    13681 gtcagcattt gcacagcatc cgtggcgcag ctcgcttggc tctatcggtg gcggcaatca
    13741 tttcgctgaa ctgcaacaag ttgatcaaat catcaacgct gaactgtttg cactggcagg
    13801 tctggatgca caacatctgc aactgttggt tcatagcggc tcgcgggggg tggggcagtc
    13861 tattttacag cggcatattg cctcgttctc gcatcatggt ttgcctcaag gcagtgacga
    13921 cgcgctacgg tatattgcgg aacatgacga tgcgctggcg tttgcgtgta ttaatcgcca
    13981 gatgatcgct ttgcgcataa tgcaacaagt taaggccacg ggaagtccgg ttctggatgt
    14041 ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa cagggctggt tgcatcgtaa
    14101 aggtgccacg ccggatgaca acggtctggt gattattccc ggttcacgtg gtgattactc
    14161 ctggattgta cagcctgttg cgaacgaaaa aacgctccac tcgctggcgc atggggcagg
    14221 gcgtaaatgg gggcgcaccg agtgtaaagg gcgtttggca gcgaaataca cggcgacgca
    14281 actctcgcgt actgaacttg gcagtcgggt aatttgtcgc gataaacaac tcatctttga
    14341 agaagcgcca caagcctata aatcggctga aagcgtggtg caatgtctgg tgcaggccgg
    14401 tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa
    14461 aaaaggatga tcttgctaca actctcctca gcgcaggggc cggaagaatg ttgtctcgca
    14521 gtgaaaaagg cgctggacag gctaattaaa gaagctgccc gacaagacgt cgcggtaacg
    14581 gtgttggaaa cagaaacggg ccgctactct gacacgctgc gttcggcgct gatttctctg
    14641 gatggcgata acgcatgggc attaagcgaa agctggtgtg gcactattca gtggatttgt
    14701 ccgagtccgt atcggcctca tcatgggcgc aaaaactggt ttctgggcat tgggcgtttt
    14761 accgctgatg agcaggaaca atcggatgca atccgttatg agacgttgcg ttcgtcgggg
    14821 ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca tttggcatcc
    14881 ggtattagcg tgaaggttca gtcagagcgt agtcagcatg ctaacaagcg gctggcacga
    14941 ttgctgattg cctggaagct ggagcagcag caacaggaaa atagcgcggt gctgaaatcg
    15001 cagcggcgaa tgttccatca ccagattgaa cgtggcaacc cgagacggac atttacaggg
    15061 atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta aacgccatcc
    15121 ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt cagcagcgtc
    15181 cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg accggtgata
    15241 gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag accacattcc
    15301 aggcccgcgt ggataatctg gatgttcggt gttttgttga acagacgctg ataggtttca
    15361 cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata tgcgcctttc
    15421 gcttcggttt tcgcgccagc cagtttaccc agcgaatcca gcatgctcac gacgtagtct
    15481 ttaccgctgt cgatcagtga acggatcaga cagtgaattt ctacgttatt atcagtcatg
    15541 gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc tgagttgcgg
    15601 atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc
    15661 agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt ctctttctct
    15721 gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag gacgtcgact
    15781 ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg
    15841 ccgccgttga aatcgataag gcgcagatcc agttcttctg catgacccgc caggaagcgc
    15901 accagcagtt tgttggcatt acccaggcca acgtggattt ccccgccgga gtgaccccct
    15961 ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct
    16021 aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat ttcaccttct
    16081 tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg taagccgaac
    16141 gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc
    16201 acgttttcgt cagccagaac tgccagtgca gaggccatac caatgccgtt atccgcaccc
    16261 agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat aggatctttc
    16321 gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg ggcctgtaag
    16381 acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag gatattacct
    16441 acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat gtattcagcg
    16501 agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa aatatcccac
    16561 agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt cgatcacccg
    16621 caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa gacaggcttg
    16681 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
    16741 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
    16801 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
    16861 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
    16921 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
    16981 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
    17041 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
    17101 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
    17161 aatgtatcca aaagcgcact ttgtcaccat ctttgcaaaa ccggctggtc gtccgctggt
    17221 tgatgattat gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg
    17281 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
    17341 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
    17401 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
    17461 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc
    17521 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
    17581 gcggtattga cccacgtgaa atcctagacg tccaggcacg tattgtgatg agcgatgctg
    17641 aacgtaccga cgatgattta tacgatactg tgattggcta ccgtggtggc aactggattt
    17701 atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc
    17761 aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc
    17821 atctgaaagg agatgacctg gcagagcaag cgcaggcttt atcaaaccgc gcctatgaag
    17881 aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttactgta cccggcggtg
    17941 cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa
    18001 tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg
    18061 cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat
    18121 ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg
    18181 taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg
    18241 ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg
    18301 tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg
    18361 acgttctggc gagtcgtttg gggatgcatg atgcttccga cgaagcgttg cgcgtggagt
    18421 tgaatcgcta ttcattaaaa gtgcaaggat tgctgggacg tcgctgccca acgccaatgt
    18481 tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct
    18541 catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg
    18601 acaaaggtct tcaggaaatc accgactgga tcgaaaaacg tttgtgttaa aaatttgcta
    18661 aattttacca atttggtaaa acagttgcat cacaacagga gattgcaatg acgttaccga
    18721 gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg
    18781 aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga
    18841 aaccggcacc ggaagtgcgt gagttctggg gctggtggat ggagctggaa gcgcaggaat
    18901 cccgttttac ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagtg
    18961 ttccggtaaa agatactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg
    19021 agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg gatgattttc
    19081 gtgacgagcc ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca
    19141 atatctatta aaactgatag gtcatgccaa ccgcgacaat atcatcatta ttaatattca
    19201 atttgttatc gctatccagt tggttgattt tataatcaac aaacgctgac atatttttgt
    19261 tgaaataata tgtagccccg acatcgatat aattgaccag atcttcatca ccgatacctt
    19321 caatatcttt ccctttcgat aagacataac ccagcgatgg acgcagacca aagtcaaact
    19381 ggtattgagc gaccgcttca aagttctgtg tcttattggc aaagccgcca gttattggcg
    19441 tcattttgcg tgtttcagaa taaaaagttg ccagataaat attattggca tcgtatttca
    19501 gacctgtagc ccatgcttct gcacgcttgc ctgtgccacg gctttgcagg ttctgctcgt
    19561 tggtgcgatc tgagttggta taggccccac taatggcgaa atcgctgccg ccaaagtcat
    19621 atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt
    19681 tcccttgata ttgcagggtt aagttcaggc catcgataac gccgaagaag tcggtgttcc
    19741 gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc gccgaggagt
    19801 cgccaccaaa ttccgggaac atatcggtcc aggcttccac gtcatacaac gcgcccaggt
    19861 tacggccata gtcgaaagaa cccaaatctt tatacttcaa tccggcaaaa gcgagacgcg
    19921 ttttttgctg tgcagtatca ctctccgctt tatttccggc aaactccgct tcccaacggc
    19981 catagccagt cagttgatcg ttaatttgtg tttcgccttt aaaaccaaaa cggatataac
    20041 tctggtcgcc atctttactg tcgttatcac tcatataatg catggcttta actttgccat
    20101 agacatccag tttattaccg tctttgttat atatttctgc ggcctgtacg gatgcagatg
    20161 ccacaatgcc catcaccact aatgccagag tgctcttttt cattttcatt cctgatttta
    20221 attaacgcgc gaatattcag cgggagagcc ccgttgaaaa caggaaagtt tttaacctga
    20281 gattgttaaa gatatattac agattaataa tattcttaaa atgtggtaat ttattaaatc
    20341 tgtaataaaa gcgtaaacaa ctgccgctag gcttgctgat cccgcgcaac aaaacgccat
    20401 gctttgcttg cagatggttg gcaaccgacg acagtcctgc taaaacgttc gtttgatatc
    20461 atttttccta aaattgaatg gcagagaatc atgagtgaca gccagacgct ggtggtaaaa
    20521 ctcggcacca gtgtgctaac aggcggatcg cgccgtctga accgtgccca tatcgttgaa
    20581 cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc ggattgttat tgtgacgtcg
    20641 ggcgcgatcg ccgccggacg tgagcacctg gggtacccgg aactgccagc gactatcgcc
    20701 tcgaaacaac tgctggcggc ggtagggcag agtcgactga ttcaactgtg ggaacagctg
    20761 ttttcgattt atggcattca cgtcgggcag atgctgctga cccgtgctga tatggaagac
    20821 cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt
    20881 ccggtaatca atgagaacga tgctgtcgct acggcagaga ttaaggtcgg cgataacgat
    20941 aacctttctg cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat
    21001 caaaaaggtt tgtataccgc tgatccgcgc agcaatccgc aggcagaact gattaaagat
    21061 gtttacggca ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga
    21121 actggcggca tgagtaccaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac
    21181 accattattg ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc
    21241 gtcggtacgc tgttccatgc ccaggcgact ccgcttgaaa accgtaaacg ctggattttc
    21301 ggtgcgccgc cggcgggtga aatcacggta gatgaagggg caactgccgc cattcttgaa
    21361 cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt
    21421 gaagtcatcc gcatttgcaa cctcgaaggt cgcgatatcg cccacggcgt cagtcgttac
    21481 aacagcgatg cattacgccg tattgccggg caccactcgc aagaaattga tgcaatactg
    21541 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagca
    21601 ggctgatgct ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac
    21661 tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa
    21721 gcgaaatcat cctcaacgct aacgcccagg atgttgctga cgcgcgtgcc aatggccttg
    21781 gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgatg
    21841 atgtgcgcca ggtgtgtaac ctcgccgatc cggtggggca ggtaatcgat ggcagcgtac
    21901 tggacagcgg cctgcgtctt gagcgtcgtc gcgtgccgct gggggtgatt ggcgtgattt
    21961 atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg
    22021 cggtgatcct gcgtggtggc aaagaaacct gtcgcactaa cgcggcaacg gtggcggtga
    22081 ttcaggacgc cctgaaatcc tgtggcttac cggcgggtgc cgtgcaggcg attgataatc
    22141 ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc
    22201 cgcgcggcgg ggctggtttg cataaactgt gccgcgaaca gtcgacaatc ccggtgatca
    22261 caggtggtat aggcgtatgc catatttacg ttgatgaaag cgcagagatc gctgaagcat
    22321 taaaagtgat cgtcaacgcg aaaactcagc gaccgagcac atgtaatacg gtagaaacgt
    22381 tgctggtgaa taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg
    22441 aaagcggcgt gacattacac gcagatgcag ctgcgctggc gcagttgcag acaggccctg
    22501 cgaaggtggt ggcggttaaa gccgaagagt atgacgatga atttctgtca ttagatttga
    22561 acgttaaaat cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac
    22621 aacactccga tgcgatcctg acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag
    22681 tggattcgtc cgctgtttac gttaacgcct ctacgcgttt taccgacggc ggccagtttg
    22741 gtctgggtgc ggaagtggcg gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc
    22801 tggaagcact gaccacttac aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa
    22861 taaaaccggg tgatgcaaaa gtagccattt gattcacaag gccattgacg catcgcccgg
    22921 ttagttttaa ccttgtccac cgtgattcac gttcgtgaac atgtcctttc agggccgata
    22981 tagctcagtt ggtagagcag cgcattcgta atgcgaaggt cgtaggttcg actcctatta
    23041 tcggcaccat caagaaaatc aataacttag cttaacttct ctaagaaatt gtatcttctg
    23101 ttatcactta caaacgccta tacgttgtta tgggctttgg tagtgtaaca cgtatgggcg
    23161 agtagttccc aggctcaacc tcgtaacaag ttcctttgaa gctggaagac gtgccaccgt
    23221 ctctaaacct tctaccaaaa ctcttagcaa gatgttccac acctggcgcg gtgtccaatg
    23281 atgctacacg tcgagatgac gttgcagagg ataaacaggc taaatgatgg gaagagtttt
    23341 aagtgcaata gtggattaaa gcccacctct tcaaaaccta acttgatcgg gaaagaatca
    23401 agctctagca cggtttcgtc caccggagta caacgggtgc ctgataagag aagaactgct
    23461 aagacttcat agcttttgtg aaaaagccct ttttagtgga tacgcctacg gcgcttattt
    23521 tttggtataa ccatcaaagg cgctttatgg ctcctattgt cttgaaaatg tatctttact
    23581 tgtattgtgt tttatgcgta gtctccctgt gtagtatgta acttgttaat tttcatatga
    23641 atacagaggc aacacgatga aaaacattgc agctatcaaa cgtaataacc gcaagattca
    23701 cgctcgtaag ttcctgtcta cgccagaggg aaaagcctgg ctagagcgta aacagcgaga
    23761 gaacgaagaa agaaaactcc ttagtgagtt gaaatggctt agagaggatt tctgaaaatc
    23821 tccagaacgc aacaggacgc attctaacgc tgttttcatt gcttggtata gggatgtata
    23881 ggtgatgtgt gtttaatgca tctgtgcgct tgttttgtgc cttattttgc attttcataa
    23941 tgcagaagag aacacggaaa accttaaaat taattattgg ggtatttaag gctattttca
    24001 g
//
LOCUS       DAZGXZ010000076        23570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000076 DAZGXZ010000000
VERSION     DAZGXZ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.076"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..231)
                     /locus_tag="ACHKJF_003103"
     CDS             complement(<1..231)
                     /locus_tag="ACHKJF_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HFP0950417.1"
                     /translation="MSNSPYSLSTRDTALSSTDCSGRRELIESKKIRITPLRERVYNF
                     VSHAKEKGISAYQILELMKKYNPNAKPATVYRS"
     gene            382..783
                     /gene="hisI"
                     /locus_tag="ACHKJF_003104"
                     /pseudo
     CDS             382..783
                     /gene="hisI"
                     /locus_tag="ACHKJF_003104"
                     /inference="COORDINATES: protein motif:HMM:NF013653.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
     gene            820..2043
                     /gene="zigA"
                     /locus_tag="ACHKJF_003105"
     CDS             820..2043
                     /gene="zigA"
                     /locus_tag="ACHKJF_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone GTPase ZigA"
                     /protein_id="HFP0950418.1"
                     /translation="MSEVSANFHGDKRLPVTVLSGFLGAGKTTLLNHILNNREGRRVA
                     VIVNDMSEVNIDAALVREGGAELSRTDEKLVEMSNGCICCTLREDLLLEVNRLAKEGR
                     FDQLVIESTGISEPLPVAETFTFAGDDGESLSTVARLDTMVTVVDAYNFLKDYGSEDS
                     IQSRGESLGEGDERSVVDLLIDQIEFCDVLVLNKVDLISEAQKEKLMAILRSLNPRAR
                     IVVSQFGQVPLGNILNTGLFDFEQAAQAPGWLKELRGEHTPETEEYGITSFVFRARRP
                     FHPTRFWQVMENELDGVVRSKGYFWLASRPEFAGSWSQAGGIARQGLGGMWWASVPKG
                     RWPEDAESLKFIMSNWIDGIGDARQELVFIGMDMNESELRNRLDSALLTDAEMAEGPQ
                     KWRHYSDPVEPWFEE"
     gene            complement(2310..3959)
                     /locus_tag="ACHKJF_003106"
     CDS             complement(2310..3959)
                     /locus_tag="ACHKJF_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014907277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP0950419.1"
                     /translation="MQSFVLMGLESAGKSTLFNILTESAASDERNFRGSTVVCREGLI
                     NDAGICLVDTPGIRFQSDSETTKLALDALNQHDGILVVLRATHAQQEWQTLCHLIPPQ
                     AKHVVILLTFADKIRKGLLEVTEYLSETSGAPVLAVNAREAGSNVRQGIVQLLLQDKP
                     APSAVSLPRQKIPVINLLAEFPQQTIFEHRWCGKVAAIVCLFLLFAVPVWGAWLLSDF
                     IQPVIDSAVIQPLKNITTSWPDVLKTLFVGNYGLFSLGLYSFVWAFPVVVLIGLSLSL
                     TDDSGLKERITATLDPWLRKLGLSGQDLIPVLSGFGCNVVAVFQSRSCSRCTRHACIS
                     MISFGSACSYQTGATLSLFNAAHQPWLFIPYLSLLFFTGAIHTRLWNGSLKPGQNQRL
                     TELTWLQWPRWRNVTWMLKNILRQFITQAMPLFLIICIVAGMLDYAGITRWLSETTAP
                     LLHLFKLPAELMPGIIFSLLRKDGLMVLNQDGGSLIQSLSTSQLLLLVWLASTLMACL
                     VTVFTIAREINWRFAAAVAGKQVLSSLVVALVISQLFIHEA"
     gene            complement(3971..5047)
                     /locus_tag="ACHKJF_003107"
     CDS             complement(3971..5047)
                     /locus_tag="ACHKJF_003107"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0950420.1"
                     /translation="MAALLRRSAIQDILVVDSHEVGASFMRWPEETRFITPSFFSNPF
                     GQPDLNSVTPDSSLALFCGEEHPGGKTYASYLKAVLDEYQIPVMAPARIAKVALLSSG
                     NFILITEAGEKLETRSLIWATGEFQFPDRQIFPGADICCHYGDVTSWKDFRKGEYIVI
                     GGYESAVDAAVNLLENGSSVKMLTRSAPWSTNHISDPSLSLSPYTRERLNRVMNHRLF
                     EIYEDADVCEVIRMPGPGSSYKVHTTNGRAWATDEVPVLATGFQCGGGARQLAAFFEW
                     NDDGYPVLTDEDCSTLFPGLYLVGPHVRHAGNIYCFIYKFRQRFPVVAESITRHLGLS
                     SEGVRDWWVLPSEPDCCADEDCAC"
     gene            complement(5172..6554)
                     /gene="cysS"
                     /locus_tag="ACHKJF_003108"
     CDS             complement(5172..6554)
                     /gene="cysS"
                     /locus_tag="ACHKJF_003108"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP0950421.1"
                     /translation="MIKLTNTLGMQKELLVPVKESKIGMYVCGVTVYDLCHLGHARTF
                     VAFDMIVRYLRFRGYDVTFVRNITDIDDKIIQRARDTGEDWRSLTERMVHEMHIDFAK
                     LGILPPNIEPRPTEHIPQIISMIERLLHRQFAYIAENGDVMFSTASAKGYGKLSGQDI
                     TMLRAGTRIEVNEYKRDPLDFVLWKRAKPGEPVWPSPWGEGRPGWHIECSAMNCHYLG
                     THFDIHGGGSDLIFPHHENEIAQSTSANDGPYVNYWLHTGMVTVNKEKMSKSLNNFFT
                     LRDILNRFDAQSVRFFLLSAHYRKPLNYSEENIHLARASLTRLYTACQFADGSRSEQH
                     DAWRARFCEAMDDDFNTPQALAILFELTREINRLCGTNDDEAAALATTLKDLGNVLGL
                     LEQSADAFLKNRSDLHPAELQLIEKLIHQRSEARRMGKWDKADRLRNELAEQGIDLED
                     VGENTSWRIR"
     gene            complement(6636..7088)
                     /locus_tag="ACHKJF_003109"
     CDS             complement(6636..7088)
                     /locus_tag="ACHKJF_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFP0950422.1"
                     /translation="MTTPVAKTYDWKQTLRTHGLRATPSTLATLQCLEHASQAMSHDD
                     LTQQLGEQAPDRVTLYRILERLMQVSIVQRYTDSARTQRFALTQRAAVGLFECDNCHH
                     VIPIEQDPALEAAIELVKSHLTGHGMTEREITLSSHGICPDCNRTITK"
     gene            7186..8385
                     /locus_tag="ACHKJF_003110"
     CDS             7186..8385
                     /locus_tag="ACHKJF_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0950423.1"
                     /translation="MKRTPLIILNGFLGAGKTTLLKNLLTQAHKRRMTVSVIVNDMSE
                     LDVDGVLIANTEIVDAASNNFVSISADSISSRSGIQKLDSALKNLLEKRSADFILLET
                     SGSSHPLPLVRYLREHTQVSLKAFLSLVDTVMLNDDYDGGKKLIPVFQEHLNKGTRGV
                     ESLLAEQIMFCNKLLLTKNDRLPFYVVTEVARAIHPLNPQVAIMAVPWGNLQLDELLS
                     MPDYDFHRVALLIDELQDAIDAVLPDETDKKYDISWRVIEDDRPFHPQRLWDTCHRFM
                     GAGVYRSKGFFWLPGRDDLALLWNQAAGSINLEFISYWKAGVLTHTDNSLTKEERAAI
                     RQQLAKMPGRFGDRRCRLTVIGESGEIDDFALALRQCLLTEEEILWWQQGGIFHDPWP
                     TKVARLA"
     gene            8456..8878
                     /gene="dksA"
                     /locus_tag="ACHKJF_003111"
     CDS             8456..8878
                     /gene="dksA"
                     /locus_tag="ACHKJF_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP0950424.1"
                     /translation="MTKPTRAEREKILAMLPEDYMNPVQQDYFRRLIRHERQEVLDHI
                     DQMKQQLQDNPETGDEGDIAIREEQLRLLFRQIDRESRLLPKYDAALMRLEKGEYGFC
                     RDTGEPIGLQRLLLRPTAELSIEAKTKEEKVEIQYRKK"
     gene            8942..9877
                     /gene="folE2"
                     /locus_tag="ACHKJF_003112"
     CDS             8942..9877
                     /gene="folE2"
                     /locus_tag="ACHKJF_003112"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016531967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFP0950425.1"
                     /translation="MSIAAKQTLCPGMLPDIQSTKGDGEGESLSWVGMEQIDLPIDIA
                     GRPVSAKVNAGINLLSSPEAEKGIHMSRLYLLLDELTQGEITPALLQHVLKAFLVSHQ
                     GRSDEASIEISGDLLLSRKSLNSNHSGWKAYPLTLSAELRQSFTVTLKVGIPYSSTCP
                     ASAALSRHVAGLQFSKDFGNRIDRLPAAEIADWLVEKGMPATPHSQRSWAWVSIRLNP
                     EAKSLPVIELIDYAEVALGTAVQTVVKRSDEQAFAVANGQNLMFCEDAARRLNNVFRC
                     APFCEAFDIRVEHQESLHPHNAVARIHWKGSKNVT"
     gene            9867..10427
                     /locus_tag="ACHKJF_003113"
     CDS             9867..10427
                     /locus_tag="ACHKJF_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008455674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP0950426.1"
                     /translation="MLRKNPSGHTPVVSTKAYIDPTAVICGRVIIHDYVYVGPYAVIR
                     ADELNADGDMDPIIIHSHSNIQDGVVIHSKSGAPVTIGSGTSIAHRAIVHGPCQVDER
                     VFIGFNSVLFNCHIQTGCVIRYNAVVDGVTLPENTYIPSTERVGPDSDLKSYSRVDPA
                     SLQFSEEVASTNVKLVEGYQKLRNEF"
     gene            10557..11423
                     /locus_tag="ACHKJF_003114"
     CDS             10557..11423
                     /locus_tag="ACHKJF_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase"
                     /protein_id="HFP0950427.1"
                     /translation="MKLFINDLTVMDFSFLDAESGLIGDSLIVDIILEGDLNAESMVM
                     DFSHAKKSIKHKIDKLADHVLIVPEQNSHIIVSHAGTTTEVAMLRKNGETQCFISGPQ
                     ESFWLVQTDNINSRCLESQIEKHLLACLPQGVKDITITLRPESINGDSYHYSHGLKKH
                     RGNCQRIAHGHRSAIRIFVDGERSHMWEQKWATRWNNAYLLSREDVVTVTTLSPRGVA
                     YWHKGLTCSSWRSSQGYFEIMLCSEVVDILPCDTTVESLALFIRQSIEHGLPAAKIEV
                     HAFEGVGKGAIA"
     gene            complement(11440..12249)
                     /locus_tag="ACHKJF_003115"
     CDS             complement(11440..12249)
                     /locus_tag="ACHKJF_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HFP0950428.1"
                     /translation="MKKAIIFLLSFYSCVGFAEPQQARSDYSIEESQAKVNKILHTTS
                     LYRNGLSYNERVAEISSRFLGTPYQAHTLIGSSSMQERLVTNPSTVDCFTFLDYVRSM
                     AHASSWQTYVSELVKTRYTNGMIDFTGRKHFFTDWAVISPRNAQDVTQDISPYTITVN
                     KQLNQKNKKQEYVKGLGIISRRISYIPASAIDKEVINKLQTGDYVGIYSTKRGLDVSH
                     VGIIIKDHNNIWFRNASSLAKNRKVVDSPFIRYMATKPGIVVLRDKTDQYP"
     gene            complement(12265..13092)
                     /locus_tag="ACHKJF_003116"
     CDS             complement(12265..13092)
                     /locus_tag="ACHKJF_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFP0950429.1"
                     /translation="MLNMLIEPFRFDFINYALIIALLVSVPCALLSVFLVLKGWALMG
                     DAMSHAVFPGVVLAWIVGLPLGTGAFVAGLSCALLTGYIKDNSRIKQDTLLGIIFSGM
                     FASGLILYVSVRPDVPLEHILFGDMLGINATDILQTGTIALFILLITGIKWRDFLLYC
                     FDIQQARVSGLRTGWLYYGLLSMISLTVVATLKSVGIILSVSLLIAPGAIAVLLTQRF
                     SNALFIAVALSALVSTTGVYLSYFIDSAPAPTVVLLFALIFIVIFIYSSFKYNEKYL"
     gene            complement(13086..13946)
                     /gene="sitC"
                     /locus_tag="ACHKJF_003117"
     CDS             complement(13086..13946)
                     /gene="sitC"
                     /locus_tag="ACHKJF_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004141220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFP0950430.1"
                     /translation="MSWLLEPFGYQYMLNAMWISAMVGGICAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAFMLGLPFSVGAFVSGGLAAGSMFLLNQRTRLKEDVIIGLIFSSFF
                     ALGLFIISLNPVSVNIQSIILGNILAVPPEDILQLTVIGIISVITLLLKWRDLLLTFF
                     DENHARAVGLHPERLKILFFLLLTLSTVAALQTVGAFMVICLVVTPGATAWLLTDRFP
                     RLVCIAVTIGSVTSFLGAWASYYLDGVTGGIIVVAQTLIFLLVFIFAPVHGLLANRRS
                     VSQSKGPRKC"
     gene            complement(13943..14764)
                     /locus_tag="ACHKJF_003118"
     CDS             complement(13943..14764)
                     /locus_tag="ACHKJF_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese/iron ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0950431.1"
                     /translation="MSQAAGITVAGLTVVYRNGHKALQNASFSVPKGSNAALLGVNGS
                     GKSTLFRAMMGFVSPSCGTISLLGMPPAQALRRNIIAYVPQSEEIDWSFPVLVEDVVM
                     MGRYGHMGMLRRAGKEDRRIVNDALERVDMLPYRHRQIGELSGGQKKRVFLARSIAQR
                     GKVILLDEPFTGVDVNTEKKIITLLGELKEEGSTLVVSTHNLTSVSEYCDYTVLVKGT
                     VLASGPVATTFTPENIKKTFSANIESESDILPVQGGSTQPNYQPSAEYEDKGRSS"
     gene            complement(14768..15682)
                     /locus_tag="ACHKJF_003119"
     CDS             complement(14768..15682)
                     /locus_tag="ACHKJF_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFP0950432.1"
                     /translation="MLQLKTVKIILLSTFITGLTAIHGAYAAEKFKVITTFTVIADMA
                     QNVAGDAAEVSSITRPGAEIHEYQPTTGDIKRAQGAQLILSNGFNLELWFQKFYQRLK
                     NVPDVVVTTGITPISITEGPYDGKPNPHAWMSPDNALIYVDNIRDALVKYDQGNAETY
                     KANANAYKQKIRQTLEPLRKQIETLPAEQRWLVSNEGAFSYLARDLGLKELYLWPINA
                     DQQGTPQQVRNVIDKVRQNKIPAVFSESTVSNKPARQVARETGAHYGGVLYVDSLSAA
                     NGPVPTYLDLLNVTTRTVVQGLHSGMKE"
     gene            15935..17893
                     /gene="thrS"
                     /locus_tag="ACHKJF_003120"
     CDS             15935..17893
                     /gene="thrS"
                     /locus_tag="ACHKJF_003120"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP0950433.1"
                     /translation="MKMSEAPITESEPLTFTLPDGSLKHVRKGATLQNVAESIGSSVA
                     KSAVYAEIDGKCVDLLEKAKNSGKLNIITVFDEEALGPIRRGCLLLLAASVKQLFPSA
                     RMVEGQLTKEGFYYDFAVDKPFTRDDLGMIEQHMTSLIAENPPIIKEQVTRAQAIDCF
                     EQRGEKFKSTELMEILADKSVTLCHLRQFTDGFNGPLVPDLRFLKNMKLLNVSGAYWR
                     GEANNVQLQRIYGTAWASKKQLDGWLEKTAEAEKRDHRKLGRELDLFHFQDNAPGAVF
                     WHPRGWTIFQSLIAYMRSRHEVAGYVEVNTPDVMDRSLWEISGHWDNYRDHMFTTQTE
                     DGRNFALKPMNCPGAVSLFKYGIKSYRDLPVRLSEFGKVHRYEPSGSLHGLLRVRHFT
                     QDDAHIFCTLQQVEGECKSILQLVLDIYKQFGFEEVAIKLSTRPEKRMGNDADWDRLE
                     NALSASLEAQGLQWSVNPGEGAFYGPKLEFVLRDAIGRDWQCGTLQVDMNLPERFDIG
                     YIAEDGSTKRPVMLHRALFGSLERFTGILLEHYVGKLPAWLSPVQAVVMTITDKQHHY
                     AEQVLKALRKKGLRCEADLRNEKIGFKIREQTLARIPFLLIVGDAEENAARVTVRDRT
                     GRCMGTLLLNEAADAIKICCQPPEVHLD"
     gene            17886..18737
                     /locus_tag="ACHKJF_003121"
     CDS             17886..18737
                     /locus_tag="ACHKJF_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallocarboxypeptidase"
                     /protein_id="HFP0950434.1"
                     /translation="MIKQSRSDIPRRESGRHVWPVKEDDTQSNDIDFYKTAFREQSRR
                     WGLSEIILSNTGDGEISLYQSVNQGADVPHVLIAAGFHGEEPAGCWGMLDFLREGSPE
                     LFDNVAVSFLPLVNLSGLRTGSRLNSLGQNPNRGFTKGAEVEPSIEGKVLLNYETLLK
                     NAASHGVLCCHEDILRHKAYLYTFEHATRLGHFSVALRDELERFFPVMEKERVDGCEC
                     EDGIIFNHFDSSFESWLFSSCSDVAACTETPGLQPFAKRAEANRYLIGAFISSILERN
                     SIGSGMS"
     gene            18734..20080
                     /locus_tag="ACHKJF_003122"
     CDS             18734..20080
                     /locus_tag="ACHKJF_003122"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011154555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP0950435.1"
                     /translation="MKPLLLTNALIINEDLRYPADILIDKGRIQKIASLIPSRTEWQV
                     IDVRGKWVIPGMIDDQVHFREPGLTHKGTIASESAAAVMGGITSFMEMPNVTPPTTTL
                     QALREKFQRASHSSLANYSFYFGATNDNLDELKALTASQACGVKVFMGASTGNMLVDD
                     EQILESIFANAPCLVATHCEHTPTIKHNEETWRARLGDAIPAGEHAAIRSVDACLTSS
                     HQAVSLAKKHRTRLHVLHITTADELALFDAAPTLEALRQKTITAEACVHHLFFNYDDY
                     EMLGHKLKCNPSVKSSFHQEALWRGVNEGIIDVIATDHAPHLLEEKQNDYFAAPSGLP
                     LVQHALPALLDMSSRGIFTPEMVVRKTSHAVAERFQLKDRGYIREGYWADLVVIDPFS
                     HQQIIREDVAYKCGWSPFEGRILSGGAVDMTLVNGHVIWNGRTIQQKYGLPLEFCR"
     gene            20141..21163
                     /gene="hemB"
                     /locus_tag="ACHKJF_003123"
     CDS             20141..21163
                     /gene="hemB"
                     /locus_tag="ACHKJF_003123"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP0950436.1"
                     /translation="MSIIIPGSFPARRLRRSRTHSFSRRLVAENQLTVDDLILPVFII
                     EGQNLRQEVPSMPGVFRLSVDMLLHDAAQFVQAGIPAIALFPCIESSAKSLMADASWD
                     PSGLVPRAVRALKKAFPELGVITDVALDAYTTHGQDGITDENGYVLNEPTKEILTRQA
                     LTHAEAGADIVAPSDMMDGRIGHIRQAFETNGLLNIQIMAYSAKYASNYYGPFREATK
                     SAVSLGKRDKKSYQMDPANAMEAMHEIAQDLQEGADMVMVKPGMPYLDIIREARKTFA
                     VPVFAYQVSGEYTMHMAAFRNGWLDEEKTVLESLMCFKRAGADGILTYFANTVAQLLN
                     QHKREY"
     gene            21316..21651
                     /locus_tag="ACHKJF_003124"
     CDS             21316..21651
                     /locus_tag="ACHKJF_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950437.1"
                     /translation="MVETSKQDRMPYLLFLCENRILAQNIDGHVIDLGGLTKQNGTID
                     WLLDSNKEKGENLKTEIEALEDIANKIDFLFLDGQFTSLPDISAEYKDKLTSAPSMEI
                     VLNELGDKP"
     gene            21693..22877
                     /locus_tag="ACHKJF_003125"
     CDS             21693..22877
                     /locus_tag="ACHKJF_003125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0950438.1"
                     /translation="MSVKKIAVIGAGVLGLSVARSLAQQGAKVTVFERSHVGAGTSST
                     TFAWINSNGKTPESYHHLNALAIDEHIRLQQERTTEGHWLKATGTYEWAAGAPEQKRL
                     QDRVSRLLELNYPVQNLSADELKRKVPEIRVGSHAGDIWYFPGECLLVPSVFMAWLVS
                     ELHVHNAELKTQSEVTELTEEKNRAEISLANGERWQGDSIVIAAGRWSAELASLLGQQ
                     LAMIDPNQPNKIACGFLGYTRPVLTQLQANLITPGLNVRPEGGGRLLLQCPDLDSFAN
                     PANPAPVDSYIGEEMHKRLRKLFNNMEMAQLERIAVGQRSRPADGLPGLGYISGKRRI
                     YLMVTHSGMTLGPLIGRLCAEEILHDKRSSLLSDFSPERLLGKTAKDFPAFTTLHFPA
                     AQ"
     gene            23241..23495
                     /locus_tag="ACHKJF_003126"
     CDS             23241..23495
                     /locus_tag="ACHKJF_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004150562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950439.1"
                     /translation="MKISKIVAFSLTMASTPALAFQAKDTTGSDLSGKQLRATEDSII
                     SNFYSQHSRQFQTTTLGTCAAVAVPGCNCPFCSMLRNQKL"
BASE COUNT         6279 a         5413 c         5855 g         6023 t
ORIGIN      
        1 agaacgataa accgtagccg gttttgcatt cggattatat ttcttcatta attccagtat
       61 ctggtatgcg ctaatgcctt tttctttagc atgactgacg aaattataaa ctctctcccg
      121 gagcggtgtg attcgtattt tcttactctc aatcagctct ctccgcccgg aacaatccgt
      181 agacgataat gcagtgtccc ttgtacttag agaatatggg ctattactca ccgggcctcc
      241 tgtaattttc aagcgatgtt atcttttctt gtcctgaaac gatggaaatc tactgatgtt
      301 ttgatcgtgc catgtaagga aagaagaact ggcaaatttt attgttacgt tataacataa
      361 cacatgataa gtgaggtgcc aatgtttctc ttactggaaa aagcccatgc aggtgcagtt
      421 tttaaacttg aagatatcct ggcatcaata ccctgggatt ctcatggatt gatcgctgcg
      481 attgcacagc aatatgacac tggagaggtg ttgatgcttg cctggatgaa tcaacaggct
      541 cttgatgaaa ccttattgac tggcagagcc tgttactggt cgcgatcccg atcatgcctt
      601 tgacgaaaag gtgagacctc cggctgcgtt cagcaagtac atgatatccg tctggattgt
      661 gacggagatg cggtactact gcttgtggat caaaaaggtg gagcctgcca cacaggtcgg
      721 cggagttgtt tttacaacgc cattaaaggc gatgagctaa ccgtgctcaa cgatcctggt
      781 taatccgaac acaacgaata tctgaggaga tgtaaggtaa tgtccgaagt cagtgcaaat
      841 tttcatggcg ataaacgcct gccggttacc gtactttcgg gatttttggg agcagggaaa
      901 acgacactgc ttaaccatat tctgaacaac cgggaaggcc gtcgtgttgc tgtcattgtt
      961 aatgatatgt ctgaggtgaa tattgatgct gcccttgtga gggaaggtgg tgcagaactc
     1021 tcccggactg atgaaaaact ggtagaaatg agcaacggtt gcatttgctg cacattgcgc
     1081 gaagatcttc ttctcgaagt taatcgtctg gcaaaagaag ggcgttttga tcagcttgtc
     1141 attgagtcca ccggtatatc tgagcctctg cctgttgctg aaacatttac ctttgcgggc
     1201 gatgacggtg aaagcctttc gacggtggcc cgccttgaca ccatggtcac tgtggtggat
     1261 gcgtacaatt ttctgaaaga ctacggttcg gaagacagca ttcagtcacg cggagaatcg
     1321 ttaggtgaag gtgatgaaag gagcgttgtt gacttgctga tcgatcagat tgaattctgt
     1381 gatgtgctgg tccttaataa agttgacctt atcagtgaag cgcaaaaaga aaaactgatg
     1441 gcgattttac gttcgcttaa tccacgggcc agaatcgttg tttcacagtt tggacaggtt
     1501 ccgctgggca acatacttaa taccgggctg tttgatttcg agcaggccgc tcaggcaccc
     1561 ggctggctta aagaactccg gggcgaacat acgccggaga cagaagaata tgggatcacc
     1621 agctttgtct ttcgcgcccg ccggcctttt catccgaccc gtttctggca ggtgatggaa
     1681 aatgagctgg atggagtcgt cagatcaaaa ggttacttct ggctggccag ccgtccggaa
     1741 ttcgcaggtt catggtcgca ggcaggtggc attgcgcgcc aggggctggg gggcatgtgg
     1801 tgggctagtg tgccgaaagg acgttggcca gaggatgctg agtcactgaa gttcatcatg
     1861 tctaactgga tagatggcat tggtgatgcc cgtcaggaac tcgtttttat tggaatggat
     1921 atgaatgaat cagaactgcg caacagactg gattccgccc tgttaacgga tgcagaaatg
     1981 gcagaagggc ctcaaaagtg gcggcattat tccgatcctg ttgagccctg gttcgaagaa
     2041 tgaatcgaag gcacccgcat ttgtcgggtg ccttcgtatg aaagcgaaaa gggggcagat
     2101 ttacaacaat tcttccccag cttttttgaa ccactgtaca acactattca gatgttgttg
     2161 cccatatagc gagacaaaac gtctggtttc ctgatgatct tgggactcaa ggactgccag
     2221 acgggatgca ataaatgcct gcgtcaaggg aattccacat tcttctgtga tgcaccggta
     2281 ccattgctgg taagtcttgg ggatcatact tatgcctcat gaataaataa ctgggatatc
     2341 accagagcca ccaccagaga acttaatacc tgttttcccg ccactgctgc tgcaaaccgc
     2401 cagtttattt cccgggcgat ggtaaatacc gtgacaaggc aggccatcaa cgtagaggcg
     2461 agccagacaa gtagcagtaa ctgagatgtg ctgagagact gtattaagct gccgccatcc
     2521 tgattgagca ccatcaaacc atctttcctt agcagtgaaa aaatgatccc gggcatcagt
     2581 tctgctggca gtttaaaaag atgaagtaaa ggtgccgtcg tttcactcag ccagcgagtg
     2641 atccctgcgt aatcaagcat ccccgcaaca atgcagataa tcaggaataa cggcatggct
     2701 tgcgtaatga actgacggag gatatttttc agcatccatg tcacatttcg ccagcgtggc
     2761 cattgtagcc aggtgagctc tgtcagacgc tggttttggc caggcttcaa cgacccgttc
     2821 cagaggcgcg tatgtattgc gcctgtgaaa aacagcagac tgagataggg gataaagagc
     2881 catggttgat gggcggcatt aaaaagagaa agcgtcgccc ccgtctgata gctgcaagcg
     2941 gacccaaagc taatcatact tatacaagca tgccgggtgc atcgcgaaca cgaacggctc
     3001 tgaaaaaccg ccacgacatt acagccgaaa ccgcttaata caggaataag gtcctgaccg
     3061 ctgaggccga gttttctcaa ccagggatcc agcgtcgctg taattctttc cttaagtcca
     3121 ctgtcgtcag tcagcgaaag agacaaccct atcagtacca ccacaggaaa tgcccataca
     3181 aacgaataaa gtcccagact aaaaagaccg taattgccga caaatagtgt cttcagaacg
     3241 tcgggccagc tcgtcgttat attcttaagc ggctgaataa ccgcactgtc aatgacaggc
     3301 tgaataaaat ctgacagcag ccatgccccc cataccggaa cggcaaacag caggaaaagg
     3361 catactattg cagcaacttt tccacaccaa cgatgttcaa atatcgtttg ttgaggaaat
     3421 tcagccagca gattaattac cggtatcttt tgacggggta aagagacagc ggagggtgca
     3481 ggtttatcct gcaacagcaa ctgcactatt ccctggcgaa cattgctgcc agcttcccgg
     3541 gcattgactg ccagtaccgg cgcacctgat gtttcactca ggtattcagt cacttccaga
     3601 aggccctttc tgatcttatc agcaaaggtc aggagtataa caacgtgttt tgcctgtggc
     3661 ggtatgaggt gacagagcgt ttgccactcc tgctgagcgt gcgtggcccg cagaaccacc
     3721 agaatgccat catgctgatt tagcgcatca agggcgagtt ttgtggtttc actgtcgctc
     3781 tgaaaacgta tgcctggcgt atcaaccagg cagatccctg catcattaat gagaccttcc
     3841 cgacatacaa cggtcgatcc cctgaaatta cgctcatcac tggcagcact ttccgtcagg
     3901 atattaaaca gggtagattt tcctgcactt tctaatccca ttaaaacgaa tgattgcata
     3961 aacacggacc tcagcacgca cagtcctcat cggcacagca gtcaggctct gaaggcagaa
     4021 cccaccagtc ccgtactcct tcagaggaca ggcccagatg acgcgtgata ctttctgcca
     4081 cgacgggaaa gcgctgacgg aacttataga taaagcaata aatattaccg gcatgccgca
     4141 catgagggcc gacaaggtac aaaccaggaa aaagggttga gcagtcttcg tccgttaaga
     4201 ccggatagcc atcatcattc cattcaaaaa aagcagccag ttgccttgct cctccgccac
     4261 actggaagcc ggtagcaagc acaggcacct cgtcagtagc ccaggcgcgt ccgtttgtgg
     4321 tatggacttt gtacgaactt cccgggccgg gcattctgat tacttcacag acatccgcat
     4381 cttcataaat ttcgaaaaga cggtgattca tcactctgtt caaacgctca cgggtataag
     4441 gggaaagcga tagactggga tcgctgatgt ggttagttga ccagggggct gagcgggtta
     4501 acatttttac cgatgagcca ttttccagca ggtttacagc ggcatccacc gcgctttcat
     4561 agccgcctat aacaatgtat tcacctttcc tgaaatcctt ccagctggtc acatcaccgt
     4621 aatggcaaca aatatctgct ccaggaaaaa tctgccggtc tgggaactgg aattcgcctg
     4681 tagcccatat taaagagcgt gtttccagct tctctcctgc ttctgttatc agtataaaat
     4741 taccggaact gagcaacgca acctttgcaa tcctggcggg agccatgacg ggtatctgat
     4801 actcatccag tacggcctta aggtaactgg cataagtttt ccccccagga tgttcttcac
     4861 cgcagaaaag cgccaatgaa ctgtcagggg tcacagagtt caggtctggt tgtccaaacg
     4921 gattggaaaa aaaagaagga gtaataaaac gggtttcttc cggccagcgc ataaaggagg
     4981 cacctacttc gtgactgtca acgaccagga tatcctgtat ggcactacga cgaagaagcg
     5041 ccgccatacc caccccggcc ggacccgcac cgacaataac gacttcgcgt ggttggttca
     5101 taaaattacc ctttgcatta cgttataaca taacaattga tattgaaatt ttttacatcc
     5161 agagggacaa cttatcggat acgccatgag gtattttcgc cgacatcttc aagatcaatg
     5221 ccttgctccg ctaattcatt tcttaaacga tcggccttgt cccatttgcc catacgtcgt
     5281 gcctcactgc gttggtgaat taacttttca ataagttgca gttctgcagg atgaagatca
     5341 ctgcggtttt tcaaaaaggc atccgctgat tgttcaagca aacccagcac attccccagg
     5401 tctttcagcg tggtggcaag tgctgcggct tcatcatcat tcgttccgca aagcctgtta
     5461 atctctcttg tcagttcaaa taaaatggca agcgcctggg gtgtattgaa atcatcatcc
     5521 attgcctcac agaatctggc ccgccatgca tcgtgctgtt cagatcgaga cccgtcagcg
     5581 aactggcatg cggtgtagag ccgtgtcagc gaggcgcgcg cgaggtgaat attctcctcg
     5641 ctataattca gcggcttacg ataatgtgcg cttagcagga agaagcgcac actctgggca
     5701 tcgaaccgat tcagaatatc gcgcagggta aaaaagttat tcagcgattt cgacatcttc
     5761 tctttattca ccgtcaccat gccggtgtga agccagtaat ttacgtaagg gccatcattg
     5821 gcggaggtgg attgggcaat ctcattttca tgatggggaa atatcaggtc agaaccaccg
     5881 ccatgaatgt cgaaatgggt accaaggtaa tggcagttca ttgcggaaca ttcaatatgc
     5941 cagccaggtc tcccttcacc ccagggtgat ggccagacgg gctcgccggg ttttgcccgc
     6001 ttccagagaa caaaatccag cggatcgcgt ttatactcat tcacttcgat gcgggtcccg
     6061 gcacgaagca tggtaatatc ctgtcccgaa agctttccat agccttttgc cgatgcagtg
     6121 gagaacatga cgtcaccatt ctctgcaatg taagcaaatt gtcgatgcag aagtcgttcg
     6181 atcatactga tgatttgtgg gatgtgctct gtgggacgag gttcgatgtt tggcggcagg
     6241 atgcctaact ttgcaaaatc aatgtgcatt tcatgcacca tccgctctgt caggctgcgc
     6301 cagtcctctc ccgtatcacg ggcgcgctga ataattttat catctatatc agtaatgtta
     6361 cgcacgaaag tcacatcgta tccgcgaaaa cgcagatagc ggacaatcat atcaaaagct
     6421 acaaaggttc tggcgtgccc aagatggcaa aggtcataaa ccgtcacgcc gcatacatac
     6481 atgccgattt tgctctcttt aaccggaact aatagctcct tttgcatacc aagcgtgttt
     6541 gttagtttaa tcattacgtt tacctcatta tttttagata gcagcacacg aacacgcctg
     6601 gcgctaatta acagtcgtcc ggaataagaa aaaaattatt tggtgatagt acgattacag
     6661 tccggacaga taccatgact ggaaagggta atttcccgtt cggtcatacc atgtccagtg
     6721 agatgtgatt ttaccaattc aatggccgct tccagggctg gatcctgctc aataggaata
     6781 acatgatggc agttatcgca ttcaaaaagt cctacggcag cccgttgggt cagagcgaaa
     6841 cgctgagttc ttgccgagtc ggtataacgc tggacaatac tgacctgcat tagccgttcc
     6901 agtattcggt aaagggtcac acgatcggga gcctgctcgc ccagttgctg agttaaatcg
     6961 tcatggctca tggcctggct ggcatgttca aggcactgca gtgtagcaag ggtggatggc
     7021 gttgcccgca aaccatgtgt gcgcaacgtt tgcttccagt catacgtttt ggctactggt
     7081 gttgtcatat ttccccctcc gagtcatgtc atttgaattt tggcaggaag ccggtattat
     7141 tgcaacttga ttgcattaac gtttagcggc ataaggctgg atgctatgaa aagaacaccg
     7201 ctcattatcc ttaatggttt tctgggggca gggaaaacta ctctgctaaa aaaccttctt
     7261 acacaggcac acaaacggcg aatgactgta tccgtcatcg ttaatgacat gagtgaactt
     7321 gatgtggatg gcgtcctgat agccaacaca gaaatcgttg atgcagccag taacaatttt
     7381 gtgagtattt cagcagacag tatcagcagc agaagcggga tccagaagct ggattccgcg
     7441 ctgaaaaacc tgctcgaaaa acggtctgcg gattttattt tactggaaac ctcaggtagc
     7501 agccatcctc ttcccctggt ccgctatctg cgagaacata cccaggtaag tctcaaagca
     7561 tttttgtctc ttgttgatac ggtcatgctt aatgatgact acgacggtgg aaagaagctt
     7621 attccggttt tccaggagca cctcaataaa ggaacccggg gagtggaaag cctgctggca
     7681 gaacagatta tgttctgtaa taagctctta ttaaccaaaa acgaccggtt acccttctat
     7741 gtggttactg aggtagccag agccatacat ccccttaatc cccaggttgc aattatggcc
     7801 gtgccctggg gaaatttaca gctggatgaa ttgctttcca tgccggatta cgattttcat
     7861 cgggtggcgt tacttatcga tgagcttcag gatgccattg acgcagtttt gccggatgaa
     7921 accgataaaa aatacgatat ttcgtggagg gtcattgagg atgaccgtcc atttcatccg
     7981 cagcggctgt gggatacctg ccatcgcttc atgggcgcag gcgtttaccg cagcaagggg
     8041 tttttctggc taccgggaag ggatgacctg gcattattgt ggaaccaggc agcagggagc
     8101 atcaatctcg aattcataag ctactggaaa gccggggtcc tgactcacac tgataattca
     8161 ttgacaaagg aagaacgcgc agctatccgt cagcaactgg caaaaatgcc tggcagattt
     8221 ggtgaccggc gctgccgcct gactgttatt ggcgagagcg gcgaaattga cgattttgcc
     8281 ctcgcattgc gccagtgttt actcactgaa gaggaaattc tctggtggca gcaggggggg
     8341 attttccacg atccctggcc cacgaaagtg gcccggctgg cttaacaggt tattttgcag
     8401 cggcagttaa tgttatgtta taacgtttca ttacgcaatt ttatcggtac aggacatgac
     8461 taaacctacc cgcgctgaac gagaaaaaat cctggcgatg ctgccagaag attacatgaa
     8521 tcctgtgcaa caggactact tccggcggct tatccgccac gaaaggcagg aggtgcttga
     8581 tcatatagac cagatgaagc aacaactgca ggataatcca gaaacgggtg acgaaggtga
     8641 catcgccatt cgtgaagagc agttacgtct gctgttcaga cagatagaca gagaaagccg
     8701 cctgttgccg aaatatgatg cagcgcttat gcggcttgaa aaaggagaat atggcttctg
     8761 ccgggataca ggcgaaccta tcggtctgca acgcctgcta ctcaggccca ccgcagaatt
     8821 aagtattgaa gccaaaacaa aggaggaaaa agttgagatt cagtaccgaa agaaataaag
     8881 cggtggctca ggcagggggc agggtaggga tgagagtaaa tatcagatca ggcaggtata
     8941 tatgagtatt gcagccaaac aaaccttatg ccccggaatg ctaccggata ttcagtccac
     9001 taagggtgac ggtgagggcg agtcattatc ctgggttggc atggaacaaa ttgacctgcc
     9061 gatagacatc gcaggcaggc cggtatcagc aaaggtcaat gctggcataa accttctcag
     9121 ttcacctgaa gctgaaaagg ggatccatat gtctcgtctt tatcttcttc tggacgaact
     9181 gacacaaggg gaaatcacac cggcattatt gcaacatgtg ttgaaagcat ttctggtatc
     9241 tcatcaggga cgtagtgatg aagccagtat tgagatttca ggggatttgt tactgtcgcg
     9301 taagtcgtta aattctaatc actccggctg gaaagcttat cctctgactc tgagcgccga
     9361 actcaggcaa tcttttacgg ttaccctcaa agtgggtatc ccttattcgt ccacctgccc
     9421 ggcttccgct gcacttagcc gccacgtcgc gggtttgcag tttagcaaag actttggtaa
     9481 caggatcgac aggctcccgg cagcagaaat agccgactgg cttgttgaga aaggaatgcc
     9541 ggcaaccccg cacagccagc gaagctgggc atgggtgagt atccgtctta atcctgaagc
     9601 aaaatcactg cctgtgattg aactgattga ttatgctgaa gttgctctcg ggacagccgt
     9661 gcaaacggtt gtcaagcgga gcgacgaaca ggcttttgcg gtggctaacg gtcagaatct
     9721 gatgttttgc gaagatgctg cccgtcggct caataacgtt tttcgttgtg cacccttttg
     9781 tgaagcattt gatatcaggg ttgagcatca ggaaagcctg catcctcata atgccgttgc
     9841 ccgtattcac tggaaaggaa gtaaaaatgt tacgtaaaaa cccatcaggt cataccccag
     9901 tggtgtcaac aaaggcctat atcgatccca ccgcggtaat ttgtggacgt gtcattattc
     9961 atgattatgt ttacgtcgga ccttatgcgg ttatccgggc tgacgagcta aatgcagacg
    10021 gggatatgga tcctatcatt atccattccc actctaatat tcaggacggg gtggttattc
    10081 actcaaaaag cggcgcgcca gtgacaatag gaagcggaac gtctattgcc caccgtgcaa
    10141 tagtgcatgg cccctgtcag gttgacgagc gggtatttat tggattcaac agcgtgttgt
    10201 ttaactgtca tattcagact ggttgtgtta ttcgctataa tgcagttgtg gatggcgtta
    10261 ccttgcctga gaatacctat attccatcaa cggaacgcgt ggggcctgat tccgatctta
    10321 aaagttattc gagggtagac cccgcttctt tacaattttc agaagaagtg gccagtacca
    10381 acgttaaact ggtcgaaggt tatcagaagt tacgcaatga attctgagta tggctcggct
    10441 agtgccgtta acgtaacacc gtattattaa cgatttgtta ttaatgtagc aaaatgatac
    10501 ttgcctcttc tttccttttg cggaagaggc ttttctgttt tattaaagag gtagcggtga
    10561 aactttttat taatgatctg acggtcatgg attttagttt ccttgatgcg gaatccgggt
    10621 tgattggcga tagccttatt gttgatatta ttcttgaggg agatctgaat gctgagtcaa
    10681 tggtaatgga tttctcacat gctaaaaaaa gtatcaaaca taaaattgat aaactggccg
    10741 atcatgtcct gattgtgccg gaacaaaata gtcatattat tgttagtcat gcaggcacta
    10801 cgacggaagt cgccatgctg cgtaagaatg gggagaccca gtgttttatt tccgggccgc
    10861 aggagagttt ctggctggtc caaacagata atattaattc ccgttgcctt gaatcccaaa
    10921 tagaaaaaca tttactggcg tgtttgccgc aaggagtaaa agacataacg ataacgttac
    10981 ggccggaaag tattaatggg gacagttatc actattcgca tggactgaaa aaacaccgcg
    11041 gaaattgcca gagaatagcg catgggcatc gctctgcgat cagaattttt gtggatggtg
    11101 agcgaagcca tatgtgggaa cagaagtggg caacgcgatg gaataacgct tatctattaa
    11161 gccgggaaga tgtcgttaca gtgacaacac tttcaccgcg tggcgttgca tactggcata
    11221 aggggctgac atgttcttcc tggcggagtt ctcagggata ctttgaaatc atgctttgtt
    11281 ctgaggtagt agatattctg ccgtgtgata ccacggttga gtcactggct ttatttatca
    11341 ggcagtcaat tgagcacggt ttacccgcag cgaaaataga agtccatgcc tttgagggag
    11401 tggggaaagg cgctattgcc tgacacacta ttctactttt caggggtatt gatctgtttt
    11461 gtctcgtaaa actacaatac ccggttttgt agccatgtat ctaataaaag gtgagtcaac
    11521 tacctttcta tttttcgcta atgacgatgc atttctgaac cagatgttat tgtggtcttt
    11581 gataatgatc ccaacatgtg aaacatccag gcctcttttt gttgaataaa tacccacata
    11641 atcacctgtt tgcagtttat ttattacctc tttatctatt gcagaagccg gtatgtaact
    11701 tatccgtcgt gagataatgc cgagaccctt aacatattct tgttttttat tcttttggtt
    11761 tagctgtttg ttgacagtaa tagtatacgg actgatgtcc tgagtaacat cttgtgcatt
    11821 tcgcggagat atgaccgccc agtccgtgaa aaaatgtttt ctgccagtaa aatcaatcat
    11881 tccgtttgta taacgagtct taacaagttc agatacataa gtttgccagc tactggcgtg
    11941 cgccatggag cgaacataat caagaaaagt aaaacaatcc acggtactgg gattagtgac
    12001 cagcctctct tgcatcgaag atgatccaat cagtgtatgc gcctggtaag gcgttccgag
    12061 gaaacgggag gatatctcag caacgcgttc attatagctg aggccatttc tgtacaacga
    12121 agtggtatga aggattttgt taactttcgc ctgactttct tcaatagaat agtcgcttcg
    12181 tgcctgctga ggctcagcga atcccacgca gctataaaat gaaaggagaa agataattgc
    12241 ttttttcata aaatatcaac ttcctcagag atatttctca ttatatttaa agcttgagta
    12301 tataaaaata actataaata tcagggcaaa aagcaaaaca accgttggcg caggcgcact
    12361 atcaatgaaa taagagaggt aaacgccggt cgttgatacc agagccgata aagccacagc
    12421 gatgaataat gcgttgctaa atcgttgcgt caaaagaact gcaatagcac cgggcgcgat
    12481 aagcaaagag acggataaaa taatccccac ggactttagc gtggcgacaa cagtaagcga
    12541 aatcatgctc agtaatccgt aatagagcca tcccgtccgt agtccactga ccctagcctg
    12601 ctgaatatca aaacagtaaa gcaggaaatc acgccattta attccggtaa tcagcagtat
    12661 aaataaggca atggtgcccg tctggagtat gtcggtggcg tttataccaa gcatgtcgcc
    12721 aaagaggata tgttcaagcg gaacatccgg acggactgag acatacaaaa tcagtccgga
    12781 ggcgaacata cccgaaaaaa tgatacccag cagcgtatcc tgctttatgc ggctgttatc
    12841 tttaatatat ccggtaagta aagcacagga taatccagcc acaaaagctc cggtgcctaa
    12901 tggcaaccct acaatccagg ccagtacaac cccgggaaac acggcatggc tcatggcatc
    12961 ccccatcaga gcccaacctt tcagcacgag aaaaacagac agcagggcgc agggaacaga
    13021 gaccagcagc gcgattatca gggcataatt gatgaagtca aagcggaaag gttcaatgag
    13081 catattcagc atttacgcgg tccttttgac tgagaaaccg atcggcggtt cgccagtaag
    13141 ccatgtaccg gggcgaaaat gaacaccagc aagaaaatga gggtctgagc gaccacgata
    13201 atgccacccg ttacgccatc aaggtagtaa cttgcccacg cacccagaaa gctggtgaca
    13261 ctgcctatgg tcaccgcaat acagaccagg cgggggaagc gatcagtaag cagccaggcc
    13321 gttgcgccag gcgttaccac aaggcagatc accatgaacg cgcctacggt ctgtaatgcc
    13381 gcaacggtgg agagggttaa cagaaggaaa aataatatct taagcctttc cggatgtaat
    13441 cctaccgccc gcgcatgatt ttcatcgaaa aaggtcagta ataaatctct ccattttaat
    13501 aacaacgtaa taaccgaaat aatgccaatg acggtaagct gaagaatatc ttctggcgga
    13561 accgccagga tatttcccag aatgattgat tgtatattta ccgaaacagg attcagcgag
    13621 atgataaata atccaagcgc gaaaaaggat gaaaagatca aaccaatgat gacatcttct
    13681 tttagacggg tacgttgatt aagaaggaac atgctgcctg ctgccagccc gccagaaaca
    13741 aacgcaccca ccgagaaggg cagtcccagc ataaaagcgc cagctactcc gggtacgata
    13801 gaatgggaca gcgcatcgcc aattagtgac caacccttca acatcagata acaggataaa
    13861 aaagcacata ttcctccgac cattgccgaa atccacatgg cattgagcat gtattgataa
    13921 ccaaaaggct ccagaagcca gctcatgatg acctgccttt atcttcatat tcagcggaag
    13981 gttgataatt tggctgtgtt gatcctccct gaactggcaa tatatctgac tcactctcaa
    14041 tgtttgcact aaacgtcttt tttatatttt ccggcgtaaa tgtcgtggcg acaggaccgc
    14101 ttgccagcac ggtgcctttc accagaacgg tataatcaca atattcggat acggaggtaa
    14161 ggttatgcgt tgaaaccacc agggtgctgc cttcttcttt cagttcgcca agcagcgtga
    14221 tgattttctt ttcggtattg acatccacac cggtaaaagg ctcatcaaga aggattactt
    14281 tgccccgttg tgcaatagaa cgcgcgagga acacccgctt cttttgccct ccggataatt
    14341 caccaatctg ccggtggcgg taaggaagca tatctacacg ctcaagcgca tcattaacga
    14401 tcctgcgatc ttctttaccc gctctacgca gcatacccat atggccatac cggcccatca
    14461 tgacgacatc ttccaccaat accgggaacg accagtcaat ttcctcggac tgaggtacat
    14521 aggcaataat attgcggcga agcgcctgtg caggaggcat cccgagaagt gaaatcgttc
    14581 cgcatgaagg ggatacgaat cccatcattg ccctgaatag tgtggatttt ccggaaccat
    14641 ttacccccag cagggccgcg ttcgaacctt tagggacgct gaatgatgca ttctgtaatg
    14701 ccttatggcc gttacgataa acaacagtta agcccgctac tgtgatgcct gccgcctggc
    14761 tcatctttta ctccttcatt ccgctgtgca aaccctgtac cactgtccgg gtagtgacgt
    14821 tcagcagatc aaggtaggtg ggaaccgggc catttgctgc gctcagggaa tccacataaa
    14881 gtacgccccc ataatgtgca cctgtttcgc gtgcgacctg acgggctggt ttattggaaa
    14941 cggtactttc gctaaatacc gccggaattt tattctgccg gactttatcg attacgttac
    15001 gaacctgttg cggggttccc tgttgatcgg cattaatcgg ccatagatag agttctttca
    15061 gtcccagatc gcgtgccaga taagaaaatg cgccttcgtt cgataccagc cagcgctgtt
    15121 cggcagggag ggtttctatt tgtttacgca gcggttcaag cgtctgacgg atcttttgtt
    15181 tataggcgtt cgcattggct ttgtaagttt cagcatttcc ctgatcgtat ttcaccagcg
    15241 catcacgaat attatctaca taaatcagtg cattatcagg cgacatccag gcatgaggat
    15301 ttggttttcc atcatacgga ccctcggtaa tgcttattgg agtgatacct gtagtgacca
    15361 ccacatccgg tacgtttttc aatcgttgat aaaatttttg aaaccagagt tcgaggttga
    15421 agccatttga aaggattagc tgtgccccct gagcacgctt tatatctccg gttgtaggct
    15481 gatattcatg gatttctgcg ccaggccggg taatcgaact gacttcggcg gcgtcccctg
    15541 ccacgttctg tgccatgtca gcaattaccg tgaatgtcgt gatgacctta aacttttcag
    15601 cagcataagc gccatgaata gctgtcagtc ctgtgataaa ggtgcttaac aggattatct
    15661 ttaccgtctt tagttgaagc atggttatct cctgatgaaa tcactgtttg ttaataattg
    15721 gtaatgttat aacatagctt atactatgca acatagcaca ggctatattt aaatgagaaa
    15781 aattatcttt atcatttaag agctgatggc acagtatttc atggcttatg caagcgctga
    15841 tatctcagac atgccacttt taatgcaaca tggttgcgtt aaaatgtgca ttaatgcaac
    15901 atagttgctg ttaatcactg agctatgttg ggatatgaaa atgtcagaag cgcctataac
    15961 agagtcagaa cctctcactt ttactcttcc tgatggctct ctgaagcacg tcagaaaagg
    16021 ggcaactttg cagaacgttg cagaaagcat cggttcttcc gtcgcgaaaa gcgctgtgta
    16081 cgcggaaatt gatggtaaat gtgtggatct gcttgagaaa gcaaagaaca gtggaaagct
    16141 caatatcatt acagtttttg atgaagaagc gttgggcccg attcgccgag gttgtcttct
    16201 ccttctcgca gcgtctgtaa aacagctgtt tccctcggcc aggatggttg aagggcaact
    16261 gactaaggaa gggttttatt acgatttcgc ggtagataaa ccttttactc gcgatgacct
    16321 ggggatgatt gagcagcaca tgacaagtct gatcgctgaa aacccgccga ttattaaaga
    16381 gcaggtcacg cgggcacagg ctattgactg ttttgagcag cggggcgaga aattcaaatc
    16441 aacggagttg atggaaatac tggcagataa gtcggtgaca ctatgccatc tcaggcagtt
    16501 cacggacggc tttaatggcc cattggttcc ggatcttcgt tttctaaaaa atatgaagtt
    16561 gcttaacgta tccggtgctt actggcgtgg tgaagccaac aatgtacagc tacagcggat
    16621 ttatggcacg gcatgggcca gcaaaaaaca gcttgatggc tggcttgaga aaacggcaga
    16681 agcggagaaa cgtgatcatc gcaagctggg tcgggagctg gatttgtttc attttcagga
    16741 caatgcgcca ggcgcggttt tctggcatcc cagggggtgg acaatctttc agtcgcttat
    16801 tgcctatatg cgtagccgcc atgaggtggc gggttacgtt gaggtcaata cgcctgatgt
    16861 catggaccgc agcctgtggg aaatttccgg tcactgggat aattatcgtg atcatatgtt
    16921 tacgacacaa accgaggatg gccggaattt cgctctcaag ccgatgaact gtcctggggc
    16981 ggtatcgctg tttaaatacg ggatcaagag ttaccgcgat ttacccgttc gcctgtctga
    17041 atttggtaaa gtccatcgtt atgaaccctc tggatcgctg catggcttat tacgggtccg
    17101 ccattttact caggatgacg cgcatatctt ttgcacgctg caacaggtcg agggggaatg
    17161 taaaagtatc cttcagttgg tgcttgatat ttataagcaa tttggatttg aagaggtcgc
    17221 aatcaagtta tccactcgtc ctgaaaagcg gatgggaaac gacgcagact gggataggct
    17281 ggaaaacgcg ctctcagcct ctctggaagc gcagggatta caatggtcgg taaatcccgg
    17341 tgaaggtgcc ttctatggcc ctaaactgga gtttgtgtta cgcgacgcga ttggtcgtga
    17401 ctggcagtgc ggaacgctac aggtggatat gaatctgcct gagcgatttg atattggcta
    17461 tattgccgag gatggctcga caaaacgccc ggtgatgttg cacagggcgc tcttcggctc
    17521 ccttgagcgg ttcaccggta tccttcttga acattacgtc ggtaaactgc ccgcctggct
    17581 gtcgccggtt caggccgtgg tcatgacgat taccgacaag cagcatcact atgcggagca
    17641 ggttttgaag gcgcttcgca agaaagggct gcgttgtgag gccgacctcc gcaatgaaaa
    17701 aattggattt aaaatccgtg aacagactct ggcccgcatt cctttcctgc tgatcgttgg
    17761 cgatgcggag gagaatgcgg cgcgtgtcac ggtgcgcgat cgtaccggga ggtgcatggg
    17821 gacgctgtta ctcaatgagg cggcagacgc tatcaaaata tgttgccagc caccggaggt
    17881 tcaccttgat taagcaaagc agaagcgata tcccccgacg cgagagcggt cgtcacgtct
    17941 ggccggtgaa ggaggatgat acccaaagca acgatatcga tttttataaa acggcgttca
    18001 gagaacagtc caggcgatgg gggctttccg agatcattct tagtaatact ggtgatggag
    18061 aaatatcact ctatcagtct gttaatcagg gggcagatgt tcctcacgta ctgattgcgg
    18121 cagggtttca tggagaagaa cccgcgggat gttgggggat gctggatttt ttgcgcgagg
    18181 gctcccctga attgtttgat aacgtagccg tgagtttttt accgcttgta aacctctctg
    18241 gtctgcgcac tggtagccgc cttaatagtc ttggacagaa tccaaaccga ggctttacca
    18301 aaggtgcaga agtcgagcct tctattgaag gaaaagtact gctcaactat gaaacgcttc
    18361 tgaaaaacgc agcaagtcat ggtgtgctct gttgccatga agatattctt cgtcataagg
    18421 cgtatctcta cactttcgaa catgctactc gcttgggtca tttcagcgtg gcattacgtg
    18481 atgaacttga acgctttttc ccggtcatgg aaaaagagcg agttgatggt tgtgaatgtg
    18541 aggatggcat tatttttaac catttcgatt cgtcatttga atcatggttg ttttcttcct
    18601 gttccgacgt cgcagcgtgc actgaaacgc cgggattgca accctttgca aaacgagcag
    18661 aagcaaaccg ttatttaatc ggcgcgttta tctcatcgat actggagcgc aacagcatcg
    18721 ggagcgggat gtcatgaagc ctttattact gaccaatgca ttaataatca atgaagactt
    18781 gcgctatccg gccgacatcc tcattgataa aggacggatc cagaaaattg cctcgcttat
    18841 tccctcccga acggaatggc aggttattga tgtgcgaggg aaatgggtga tccccggtat
    18901 gattgacgat caggttcatt tccgcgaacc cggtctgacg cataagggaa ccattgccag
    18961 tgaatcagct gccgctgtaa tgggaggcat taccagtttt atggaaatgc ctaacgttac
    19021 gccaccaaca acgacccttc aggcgctgcg tgagaaattt caacgtgcct cgcattcttc
    19081 gctggcgaac tacagttttt acttcggcgc gacgaacgat aatcttgacg aactgaaagc
    19141 gcttacggct tcgcaagcct gtggggtgaa ggtgtttatg ggagcttcta ccggaaatat
    19201 gctggtggac gatgaacaga tcctggaaag tattttcgcc aacgcaccct gcctggtagc
    19261 aacacattgc gagcataccc cgacaataaa gcataatgag gagacatggc gggctcgttt
    19321 gggggacgct attcctgctg gcgaacatgc cgctattcgt tctgtggacg cctgcctgac
    19381 atcttcccac caggccgtct ctctggcgaa aaaacatcgt acccgattac atgtcctgca
    19441 tatcaccacc gcagatgaac tcgctctctt tgatgctgct cccacgctgg aggcgctgcg
    19501 ccagaaaacc attacagctg aagcctgcgt tcatcacctg ttttttaatt atgacgatta
    19561 tgaaatgctg gggcataagc tcaaatgtaa tccatccgta aaatcctctt ttcatcagga
    19621 agccctctgg agaggcgtta atgaaggcat tattgacgtt atcgcgactg accatgcgcc
    19681 gcatttgctg gaggaaaagc agaatgacta ttttgctgcg ccttccggct taccgctggt
    19741 gcagcatgct cttcctgcac tgctggatat gagcagcagg gggattttta ccccggaaat
    19801 ggtggtgaga aagacaagcc atgctgtagc cgagcgcttc cagctcaaag atcgtggcta
    19861 tatcagagaa ggctattggg cggacctggt ggttatcgat ccgtttagtc atcaacagat
    19921 aatccgggag gatgtggcat acaaatgcgg gtggtccccc tttgaaggcc ggattttgtc
    19981 aggcggggcg gttgatatga cgctggtaaa tggccacgtt atctggaatg ggcgcaccat
    20041 acagcagaag tacggcctgc cactggaatt ctgtcgctag ccacgtattt attttgctca
    20101 ggcataactt ctccccgaga attcagaaga ggcaaatctt gtgagtatta ttattcctgg
    20161 ttctttcccg gcacgacgac tgcgccgttc tcgcacccat tctttcagtc gccgtctggt
    20221 cgctgaaaat cagctgaccg tagatgacct gattttaccg gtattcatta ttgaggggca
    20281 aaacctgcga caggaagtgc cgtcgatgcc gggcgtattt cgtctgtcgg ttgatatgtt
    20341 gcttcatgat gccgcgcaat ttgttcaggc aggtatacct gctattgcac tatttccgtg
    20401 tatagaaagt tctgctaaat cgctgatggc agacgcttcg tgggatccat ccggtttagt
    20461 accgcgagct gtaagggcgc tgaaaaaggc ttttcctgaa ctcggggtga taactgacgt
    20521 tgctcttgac gcctatacga cacatggtca ggacggcatc actgacgaaa atggatatgt
    20581 acttaatgag ccgacaaaag aaattctcac cagacaggcg cttacccacg ccgaagcagg
    20641 cgcagatatt gtcgctccaa gcgacatgat ggatggccgc atcggtcata tcagacaagc
    20701 gtttgaaacg aatggactgc ttaatatcca gataatggcg tattcagcaa aatacgcgtc
    20761 gaactattat ggtccttttc gcgaggcgac gaaatctgct gtatcactgg gtaaacggga
    20821 caaaaaaagc tatcagatgg atccggcgaa tgcaatggaa gctatgcatg aaattgccca
    20881 ggatttgcag gaaggggcag acatggtgat ggttaagccg ggcatgcctt acctggatat
    20941 tatcagggaa gccaggaaaa cttttgcagt acccgtcttt gcctatcagg tttcagggga
    21001 atatacgatg catatggccg ctttccgaaa tggttggctg gacgaagaaa aaacggtgct
    21061 tgaatcactt atgtgcttca aacgcgccgg ggcggatgga atactgactt attttgccaa
    21121 tacagtagcc cagttgctta atcaacataa acgggaatat tgattccctg ttgacaaaat
    21181 tccttagtcg ctaagcagga gcaccgctga gagtaaacgt cataatcagc gagaatttct
    21241 atactttaat tcttatcttt attgcgaatt ctgacgaatc gtttgtatca gcccaataat
    21301 ctggaggaag cagttatggt cgaaaccagt aaacaggacc gtatgcctta tctacttttc
    21361 ctgtgtgaaa accgcattct tgcacagaat atcgatggtc atgtcattga tcttggtgga
    21421 ttaaccaaac aaaacggcac cattgactgg cttctggata gcaataagga aaagggggaa
    21481 aatctcaaaa cagaaatcga agcgctggag gatattgcca ataagataga ttttcttttt
    21541 cttgatggtc agtttaccag ccttcctgac atatcggctg aatataaaga taagcttaca
    21601 tcagcccctt caatggaaat cgtattgaat gaacttggcg ataagccata agaaagccca
    21661 acgtcaggaa gctgataaaa aggaggaaag gcatgtcggt caaaaaaata gcagttattg
    21721 gtgcaggcgt actcggcctg tccgtggccc gctccctggc gcaacaggga gcaaaagtca
    21781 ctgtttttga gcgtagccat gtgggggcgg ggacaagcag cacgactttt gcctggatta
    21841 attcaaatgg caaaacacca gaaagttatc atcatctcaa tgccctggcg attgacgagc
    21901 atatccggct acaacaggaa cggaccacag aaggtcattg gctaaaagcg actgggactt
    21961 atgaatgggc ggctggtgcg ccagagcaaa aacggttaca agaccgggtc agccgtcttt
    22021 tggaactgaa ctatcctgtt cagaaccttt ccgcagatga acttaagcgc aaagttcctg
    22081 aaatccgcgt gggatctcat gccggagata tctggtattt tccaggtgaa tgcttactgg
    22141 taccttccgt ttttatggca tggctggtgt ccgagcttca tgttcataac gccgaactga
    22201 aaacacaaag cgaagtgaca gagttaactg aagaaaaaaa tagggcagag ataagccttg
    22261 ccaatggcga acgttggcag ggagacagta tagtcattgc ggcgggaaga tggtctgccg
    22321 aactcgcttc gctacttggg cagcaactgg caatgatcga tccgaaccag ccgaataaaa
    22381 tcgcatgtgg ttttctgggc tatacgcgcc cggtgttaac ccagttacag gcgaatttaa
    22441 tcacgccagg gttaaatgtc cggccagaag gaggggggcg attactgctt cagtgtcccg
    22501 accttgacag ctttgccaat cctgcaaatc cggcaccggt agatagctat atcggtgaag
    22561 aaatgcacaa aaggctacgg aagctattta ataatatgga aatggcacag ctggaacgta
    22621 tagctgttgg gcagcgttcc cgaccggcag atggtcttcc agggcttggt tatatctctg
    22681 gtaagcgacg aatctacctc atggtgacgc acagtggtat gactctgggg ccgttgatcg
    22741 gacggctttg cgcggaagag attctccacg ataagcgttc ttcgcttttg agtgatttct
    22801 ctcctgaaag attacttggg aaaacggcaa aggattttcc tgcttttact acgttacatt
    22861 tcccggctgc acagtaattt ttcagtcgat gttcaccact gaaatgagga tcagaaagct
    22921 aataaactag atcctgttca gcggcattaa caaaaagcct gtccaacatg ggcaggcctg
    22981 aagcgtttaa gcttgcaaaa atacgacaag gtgtgtgatg tcaggctata tctggccatt
    23041 atagcccgcg tacgcattgg ttacagtcag ccagaacgcc ttctgtagag gaacatcact
    23101 acccaatagt atcatttttt tgtgggtgtg ttatgttatt ttataacatc caggctcata
    23161 ttccgcatac cgaaagataa ctagttgtta tgcgttttct catactgtag tttcagagga
    23221 agatttaaca agaggttaac atgaaaatca gtaagatagt tgcattctcc ctgacaatgg
    23281 cttcgactcc ggctctggca tttcaggcga aagatacgac tggttcagat ctttcaggaa
    23341 agcagttgcg cgcaacagaa gacagcatca tcagtaactt ttacagccag catagcaggc
    23401 agtttcagac gacgacttta ggaacctgtg ctgctgtggc agtacctgga tgcaattgcc
    23461 ctttctgttc aatgttgcgt aatcaaaagc tttgagagag ttaaataaat atattacttg
    23521 taagagctga aagctgaaaa gattctggtg taatcaacta gagcagtaaa
//
LOCUS       DAZGXZ010000077        23133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000077 DAZGXZ010000000
VERSION     DAZGXZ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.077"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(473..1474)
                     /locus_tag="ACHKJF_003127"
     CDS             complement(473..1474)
                     /locus_tag="ACHKJF_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0950440.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1582..1878
                     /locus_tag="ACHKJF_003128"
     CDS             1582..1878
                     /locus_tag="ACHKJF_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0950441.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2001..2426)
                     /gene="arsC"
                     /locus_tag="ACHKJF_003129"
     CDS             complement(2001..2426)
                     /gene="arsC"
                     /locus_tag="ACHKJF_003129"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP0950442.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2439..3728)
                     /gene="arsB"
                     /locus_tag="ACHKJF_003130"
     CDS             complement(2439..3728)
                     /gene="arsB"
                     /locus_tag="ACHKJF_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP0950443.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3782..4135)
                     /gene="arsR"
                     /locus_tag="ACHKJF_003131"
     CDS             complement(3782..4135)
                     /gene="arsR"
                     /locus_tag="ACHKJF_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP0950444.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            complement(4674..4847)
                     /locus_tag="ACHKJF_003132"
     CDS             complement(4674..4847)
                     /locus_tag="ACHKJF_003132"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950445.1"
                     /translation="MHPLTHPLPVTAHALLLDNGILTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            4875..4958
                     /gene="dinQ"
                     /locus_tag="ACHKJF_003133"
     CDS             4875..4958
                     /gene="dinQ"
                     /locus_tag="ACHKJF_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP0950446.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5012..6364)
                     /gene="gorA"
                     /locus_tag="ACHKJF_003134"
     CDS             complement(5012..6364)
                     /gene="gorA"
                     /locus_tag="ACHKJF_003134"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP0950447.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6436..7278)
                     /gene="rlmJ"
                     /locus_tag="ACHKJF_003135"
     CDS             complement(6436..7278)
                     /gene="rlmJ"
                     /locus_tag="ACHKJF_003135"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP0950448.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7481..9523
                     /gene="prlC"
                     /locus_tag="ACHKJF_003136"
     CDS             7481..9523
                     /gene="prlC"
                     /locus_tag="ACHKJF_003136"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP0950449.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9531..10283
                     /gene="rsmJ"
                     /locus_tag="ACHKJF_003137"
     CDS             9531..10283
                     /gene="rsmJ"
                     /locus_tag="ACHKJF_003137"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP0950450.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10332..11801)
                     /gene="dtpB"
                     /locus_tag="ACHKJF_003138"
     CDS             complement(10332..11801)
                     /gene="dtpB"
                     /locus_tag="ACHKJF_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP0950451.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11967..12020
                     /locus_tag="ACHKJF_003139"
     CDS             11967..12020
                     /locus_tag="ACHKJF_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950452.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(12119..12553)
                     /gene="uspA"
                     /locus_tag="ACHKJF_003140"
     CDS             complement(12119..12553)
                     /gene="uspA"
                     /locus_tag="ACHKJF_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP0950453.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12944..13279
                     /gene="uspB"
                     /locus_tag="ACHKJF_003141"
     CDS             12944..13279
                     /gene="uspB"
                     /locus_tag="ACHKJF_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP0950454.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13350..14849)
                     /gene="pitA"
                     /locus_tag="ACHKJF_003142"
     CDS             complement(13350..14849)
                     /gene="pitA"
                     /locus_tag="ACHKJF_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP0950455.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15081..16283
                     /gene="yhiN"
                     /locus_tag="ACHKJF_003143"
     CDS             15081..16283
                     /gene="yhiN"
                     /locus_tag="ACHKJF_003143"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0950456.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQELAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAVQKQ"
     gene            16426..17268
                     /locus_tag="ACHKJF_003144"
     CDS             16426..17268
                     /locus_tag="ACHKJF_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFP0950457.1"
                     /translation="MARGRRLKSYQDYENALGDGIGLGYGQSYQPWLRAQDVRSHGNR
                     SIVFGLKTFRNHHFFSSVESNFFYLAEFNDLVIDIREQFPLIPLLLTHQIANHLDVQH
                     PLVRGVRGVPADVLNVMTTDFLLTLRMPGGGLRYKAIAVKQNESIPKREAEKLEIERM
                     FWQLIDVEFQIYTGSELNHVTGQNICWATSVLRNGSEFFDKYPLDKILWRLKPDVYPI
                     VELRAMISSIFDVDEQEAIMLLQAIIGLKMINVDLSYPILETGLIKIISNGYCIGLNA
                     NGYY"
     gene            17255..19381
                     /locus_tag="ACHKJF_003145"
     CDS             17255..19381
                     /locus_tag="ACHKJF_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0950458.1"
                     /translation="MDIIRNSVWQSHDSDVLSDGLYRVLDFDRDTDLLILFEIKKERT
                     SKPIPFSLSQFTYGVDSQRITHKKYTPPSYMLIDERKINDEDRKRRDVNYKIIKELVE
                     DKIFVFDYAIHKKSHLLTEYSRKKQIPQRTIRTLLTLYWKHGQDIYALLPAYPNCGAA
                     GKSRIRHEVKLGNAKKNRVLPNIRSKVYILNEGDIENIKKSLTRFHYKTHGETIKKTL
                     VRHIELYFKDEIKKANAENRAPYIPSLKQFTYWNRKLFSKEFSINKKNSKKEIALKMR
                     GLLGSAANTTVLPGDVFEIDSTVADVHLVSSLNRRKVIGRPTIYTVVDRASRMIVGLH
                     VSLYHASWRAARQALANCFMPKKEYCRIFGVSINEDEWPCSHIPLTLMCDNGEMIGLK
                     PQDKMTPLTKLEFAPVGRGDRKSIVERCFGILNDEAIHELTGATRRGKIVKGEPTPQS
                     RACLTIQEVTSVLIREILAHNQRTYEELAYINPLLIEHDLIISPKNSWMISVKQGRFS
                     GRVVTADEVIARLLIPVTANITAGGIQYNNLFYECDPDIASAARVFGRTSCEARIDDN
                     CVDYLYVRFDKNSVFRKHYLLKKRDVFKGKAHLDTDVMADWVDTQKEINAFTLNVFNN
                     SKVKDKINRQGIERLNDISDPRRVHGKDIRRNRKAELDLLGRTNLPNEKNEPVLPSSS
                     NILLLPGREEKQRWLNAKKKQEDAEK"
     gene            19378..20823
                     /locus_tag="ACHKJF_003146"
     CDS             19378..20823
                     /locus_tag="ACHKJF_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0950459.1"
                     /translation="MNMLRSVSAIYKEPGLPEYRGNPLIEALPEALSDDEVFFEMSYF
                     PEIDEDIRWTAPAKIREQYVDRIKKFRCPQTNLIEAYRMIYRALRESYAARNPLKSGT
                     VQYLHYYGNERPDIEPDSGYFKSQAETITIVGMSGSGKTTMIEQIMDYFPQVIEHSSY
                     KGAFPGLSKQIVWLKINCPYNSSVRDLCEEILLKLDDASGIERTTPEIRNGPLARQIA
                     QRIKSSFLGILVIDEMQRLKFSRTGGENKLIDFLHEIVDSMGVSMVFCGNPPFDETLA
                     RKMRIARRAESGGYMNILNVRYDSQDWQSFIHYLWPLQWTNIETPLSDELNEKLFVLS
                     KGNIGLAQMIYRRAQLKVIGSGNELISSVVLSSSVPVLASHTEEYQEALILGAVGGYD
                     DKYCSVSHAGEASTDFTADRHAKALIPGDIDRPQHKEFAQKIDEAMQNFESKIISFDR
                     ERIISRAADNNETYSSLQRCGLLNTDPLNEL"
     gene            20866..22419
                     /locus_tag="ACHKJF_003147"
     CDS             20866..22419
                     /locus_tag="ACHKJF_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001582167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsD family Tn7-like transposition protein"
                     /protein_id="HFP0950460.1"
                     /translation="MILLPVLPDETLFSRICRSVTVYGMSPSTMLQILFGKSDVSVHP
                     ILSSGLNDISLHTSESAEQLLYEQTLLPLFSWALPSSRKEIMDLRTTPGRLNRLCRLS
                     NFSSGLQTVLKFCPVCAREDAYHHGVTYWHLLHQLRGITTCYRHQILLESICVPPSPH
                     IRKGLMPPVSYIEHLSTEFEFDFAKFCYEFINMIRRGDANHPDYMDILKQLNLLSVSG
                     NIKKGVFYSHVYAKCRLLDSGSSAILPESISDYHYWEPLLRNQSCQHPTKHLLLCYCL
                     LRITWPVHADIHKKSKSIAFDDDTAQFCLLSEKHNSINSLAKELNKSRCFIKGVIHKK
                     YVKNYKCSIKINILTEFLIKSMAVRGFSLASIAKKNSLSEGSVSSVISSFYGLCSWRK
                     KCKRDSLRRRHKQKILRFMHGLTGPVTRKVVRANCYASFFWLNRFESQWLDFYLPKES
                     RRCNVKRVNWDERDKFSLSLIKNFLAQGQCSLSLSRLDSVLGGHGWLLKNRDKLPVTM
                     ALINMSKSK"
     gene            22435..>23133
                     /locus_tag="ACHKJF_003148"
     CDS             22435..>23133
                     /locus_tag="ACHKJF_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950461.1"
                     /translation="MIYENELWHSFLLRTQIIYNIQNYRNIISAHGALRYDAYPRPEL
                     INVYKLHRTQDLYDILTTVNGFILTSRMIYSPYGVYSYFDAMEDAHNKGFNIINTYPV
                     VNISRIRYCHSCILEDIRIKGVGYLRHRWQFESRCCHHNQSLYEVCFDNYSGAVKGLC
                     ELMVSGLNPSGCYSLVVDVFNPFQTPVYLLPCSDGRVRTWILENKDELVNFLCSIFKN
                     KSSSGLKRVIDVKIM"
BASE COUNT         6292 a         5318 c         5721 g         5802 t
ORIGIN      
        1 ctgatataat tacctttaaa ttccctttca tttccacccg gtaatatttg tgtataaaaa
       61 aaggcagtga tagtatcgta aaggaaaacg ttaacaacta tcaaaaagcg ctcattttaa
      121 aaagttatta tgttctccga tagtgtgtga agtaatattt ttgtgatgtt ttagatgtgt
      181 aataatgaac caggttaaag tgtatttaaa aatgtgttgt attagcgact gaaatctgcc
      241 ttttaaaaag gaggagggta ctgagacaaa gagtcattgc ttctccgata aaatgtcaga
      301 gaagcatgat tacgtggtta ttgtctgttg atgacttgtt gaccgagccg tcatcattct
      361 caatgcatta gttaaatcat ttgtgatttt taaggattat cctgtctatg attttctact
      421 tagaaggata tggcactaac tatgcatctt atagtttact gctggcatct atttaaaata
      481 gcaattgagt caaatagccc atcaagatag ccatacctaa tataaccgtc aggaatgcag
      541 ctatcatcgg cattctgaac attgatttaa gtaatatcac ttccgtcagg cttgcgccgg
      601 cactgccgat gattaatgcc attaatgctc ccagacccat tccttttgtc atcagaacag
      661 atgccagagg gataactgcc tcagcccgga tatacagcgg aataccgaca acggcgctta
      721 atgggatggc gaagggatta tctgctcctg catgagcggc aatccacgct gagggaataa
      781 agccataaat aaaagatcct attaatactc ctaacaaaag atatggcagt acatctttaa
      841 actgttgcaa cgcatctttg gtggctagtt ttatgggatt aatacttact acagctttgg
      901 tactgcaaca ggtaactacg gggttctcaa tggctttagc cgctggacta caacaactca
      961 cttttatggg cgtatatgtc gttcccggcg aagtttttgc tagagcacaa caatttgctg
     1021 atgagctttt actggcaatg atatgacgtt caaaccccag agaatccagg ataatactgg
     1081 caagaacgga gacgccggcg gcgataatcg cgtacaacaa ggtgactttc caaccaaagg
     1141 tcatccacat tagtccgacg ataatgggat taagtaatgg tgaaacaaat aaaaaagtga
     1201 gagtcggacc aaacccggct ttcgctgaca acaatccacg taacatgggt attgtcgagc
     1261 aactacagaa cggggtaacg gctcccaaca gagcagccag gagatagcct ctcccttttc
     1321 tggcccccat catctgctgg attttatggt ctggcacctt ttgtcttatc aggctgacac
     1381 cggcactaat cactataaac agcaacgaaa gctcgacggc gagaaacaca aacatctctg
     1441 cggcatcttg cagcatcgca agccaactac tcatacgtaa cctcattgga taaatctata
     1501 tttccagaat aatcgaaatg aaattcagat caagatattt ctggtattat cgaaatatca
     1561 accagacaaa gggggtgtca tatgcagttg gaagaagttg ctaaagcgct gaaagagcta
     1621 ggtcacccga cccgtttgtt catattcaaa cacctggtca aagcggggga gcaaggtttg
     1681 ccggtcggtg agttgcaaaa gcagcttggg atacccggct ctacactcag tcaccatatt
     1741 tcagcactgg tatcggtagg gctggttaaa caaaaccgtg agagtcggac gctgatgtgt
     1801 gtttcgcaat acgcaatgct ggaagcgata attgaattct tacgcgagga gtgctgcgta
     1861 aatagcaaaa ttgcctgatg cgctacgcgc tacagatttc atgccatgca cggttttgta
     1921 ggtcggataa gacgcgttag cgtcgcatcc gacataaaca aagcgcactt taccaactat
     1981 gggcagtgga tatcgccgct ttatttcaac cgcttacccg cttcatcaac cactttctcg
     2041 ccatcttcct tggagaatgc gcctttttgc gcatctggca gaatttccag caccacttct
     2101 gaagggcggc acaggcgagt tcccagcggc gtcaccacaa tcgggcgatt aatcagaatc
     2161 gggtgctgaa gcataaagtc gattaaccga tcgtcagtaa atttatcttc tgcaaggccc
     2221 agttcctcat acggctcgac gtttttacgc aacagcgcgc gtacggaaat ccccatatcg
     2281 gcaatgagtt tgaccagctc atcgcgcgtt ggcggtgttt ccagataata gataatggtc
     2341 ggttctgtac cgctgttgcg gatcatctcc agcgtattac gcgacgtacc gcaggccggg
     2401 ttgtgataaa tggtaatgtt gctcatatca atatctcatt acaaagtgaa agagagacgt
     2461 agcgccagcg cagccagcgt cacaaaaagc accggcaggg tcatgataat ccctgtacgg
     2521 aagtaatatc cccagctgat agtcatattc ttctgcgaaa gtacgtgcag ccagagtagc
     2581 gtagccaggc taccaattgg ggtaattttc ggtcccaaat cgcagccaat cacattggca
     2641 taaaccatcg cttctttgat aacgccagat gccgtgctgc catcaatgga caacgcgcca
     2701 accagtaccg tcggcatatt gttcataata gaagagagga aggcggtgag gaatccggtg
     2761 ccgagcgtcg cggcccacag gccgttatcc gccagcacgt tgagtacgcc agaaagatat
     2821 tccgttaatc cggcattgcg caggccataa accaccagat acatgccgag cgagaagatg
     2881 acaatctgcc agggggcacc gcgcaggact ttacccgtat taatcgcatg accgcgttta
     2941 gcgacgacaa ataatatcag cgcgcccaca gctgcaatgg cgctcaccgg aatgccgagc
     3001 ggttccagga cgaaaaatcc caccagcaga agcagtaaaa caacccagcc agttttgaac
     3061 gtagcaggat ctttgatcgc ttctgcggga gatttcagca gcgccatatc gtagttctgc
     3121 ggaatatctt tgcgaaaata gagatgtaac atcaccagcg tggcaacaat cgcggcgata
     3181 tccaccggca ccatcaccga ggcgtattcg cgaaagccga ggccaaagaa atcagcggaa
     3241 acgatattca ccaggttgga gacaataagc ggcaggctgg cggtatcggc aatgaatccg
     3301 gccgccatca cgaacgccag cgtagtgcct ttactgaacc ctaaagccag cagcatggcg
     3361 atgacaatcg gtgtcaaaat aagcgccgcg ccatcattgg caaacagggc ggcaacggca
     3421 gcaccgagca ggacaatcca ggtaaacagc aagcgaccac gaccattacc ccagcgtgag
     3481 acgtgcagcg ccgcccattc aaaaaagccg gactcatcca gcagcaggct gatgataatg
     3541 acggcgataa acgcagccgt cgcgttccag acgatattcc acaccaccgg aatatcaccc
     3601 ggatggacca cgcccgtaac taacgccagt actgcgccga gcgttgcact ccagccgatg
     3661 cctaaacctt tcggctgcca gataaccaat acgatggtca ggacaaagat agcgcctgcc
     3721 agtaacataa tgcctcccgg ataaaacaca tctgaaaatt catatgtgtt tagctaaatt
     3781 tttaactgca aatgttctta ctgtccccgg aacagttttg tcgagccagg ttgcggacaa
     3841 tcgcctgaac cttttcctgt tcacatcgcc aggcctcatc aataattttc gccgcccatg
     3901 ctggaatatg cggtgataag cggtaatgaa cccacttacc ttgcttgcgg tccagcaata
     3961 gcccgctttc acgcagcaat gccaggtggc gggagatctt gggctgcgac tggtcgagag
     4021 cagtgcagag atcgcagacg cataactctc ccagttcgct gagcagtaaa acgatgccca
     4081 gacgggtttc atcagcaaga attttgaaca attggatggg taacagaaat gacatattgc
     4141 gctcctgatt gttgcaggta gtgtctctct tcgaagcgga taagtcaaaa acatatatga
     4201 cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg aattttgagt tggctattca
     4261 tttgaaagga ggtctgaatc aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt
     4321 ccagccctga gttggtggct ctggctggag tgagagagtt cttatctaac agctcaatac
     4381 tattagccag tggctaacat tgcaagaatt agcgatttct tcagctggcg gagggggcac
     4441 ttcggtatat atcgcaatga cgatttatat actttcactg ggtcatcgtc atattaagcc
     4501 ttgtccgaat ccggatgtaa aaaaaccgac tttgcgtcgg ttttttactt tccagccctg
     4561 agttggtggc actggctgga gtgagaatgc cattatctaa cgcatcaatg ctaataccat
     4621 actgaaacta ttgcaaggac atgctggttt tataacctga atgtactgta tgattatcca
     4681 gttagctctg aggcattttc actctggtaa tgcgcataaa cgctttcaaa gtcctggtca
     4741 gaagtacggg tggtgccgtt aactgatgct ctggccggag tgagaatgcc attatctaac
     4801 aatagggcat gtgccgtgac aggcagtgga tgagtaagcg gatgcattct cactccatcg
     4861 catggagaaa acgggtgatt gataaagcaa tcatcgttct tggggcgtta attgcgctgc
     4921 tggaactgat ccgctttctg cttcagcttc tgaactgata gcggaaacgt aattaagggc
     4981 taagagcaca ctactcttag ccctttaaca tttaacgcat tgtcacgaac tcttctgccg
     5041 ccgttgggtg aatggcgacg gtattgtcga agtctttttt ggttgcaccc atcttcagcg
     5101 ccaccgcgaa gccctgcaac atttcgtcca taccaaagcc aatgccgtga ataccgacaa
     5161 tcttctcttc cgatccaacg cacaccagct tcatgcggca cggctggcgg tgagtggtga
     5221 cggcggtata catcgcggtg aaagaggatt tatacacttt cacctgatcg tcgccatact
     5281 gctcgcgcgc ctgcggttcc gttaaaccaa cagtaccaat cggcggatgg ctgaagacca
     5341 cggtcggaat gttgctgtaa tccagatgct catccggctt gttattaaac aggcgttcag
     5401 agagacggcg acccgctgca actgccaccg gtgtcagctc cactgcaccc gtgttatcgc
     5461 ccaccgcgta aataccttca atattggtgt tttgatattt atcgacgacg atatagcctt
     5521 tttcgttagt tttaacgcca gcggcttcca ggttgatgtt gtcattggca ggctcgcgac
     5581 caatcgccca aatcaggcaa tccaccgttt cactgcgacc atcttccagc tccagcgtca
     5641 ggctaccatc ggcatttttc actaccgctt tcgggatggc gttggtgtgc agctgcgggc
     5701 cttcggcgtt catcacttcg accagcgttt cggaaatcat cgggtcgaag ctgcgcagcg
     5761 gcgcatgttt acgcacaaac agatgcgttt tcgcgccgag gccgttaatc acgcccgcca
     5821 gctcaacggc gatgtaaccc gcgccaacaa ccgccacgcg ctctggcaaa gcaggaaggg
     5881 cgaagaagcc atcagaatca ataccgtatt ccacgcccgg aatatccggg tggctcggac
     5941 gaccgcctgt ggcgatcaga atatgatcgg ccgtgatggt ttcgccgttt acctccagcg
     6001 ttttggcatc aacgaagcgg gcaaagcctt tgattacatc aacgttattt ttaccgagca
     6061 cgttttcata ggaagtatga atacggtcga tataggcggt acggctggcg atcaacgttt
     6121 cccagttgaa tttattgata gtggtatcaa aaccataatc cgggccgtac atatggatcg
     6181 cttcacggat ttgcgccgcg tgccacatca cttttttcgg cacacagcca acatttacgc
     6241 aggtgccgcc cagctctttg gcttcaatca gcgcacattt ctggccgtac atagccgcgc
     6301 ggttgatgga ggcgataccg ccgctgccgc cgccgatggc gatgtaatca tagtgtttag
     6361 tcatgacaaa gtgtccttat cgttgattac cgcgattgta gcgcgagacg taataggtgc
     6421 cagcaatggc tgcaattact ccggcacgat ccagcttacg gtggcgtgcc cggtgcctgc
     6481 cggaaccagt ttgctgtgca gccacggcag cacgttattc atctgctgtt ccagtttcca
     6541 cggcgggtta atcacaatca tgccggaagc ggtcatgcca cggcgatcgc tgtctggcag
     6601 taccgccagt tcaatttgca gaattttgcg aataccggtc gcttccagat cgtggatcat
     6661 gcgcttaatt tgctgacgca gcaccaccgg ataccacagt gcgtaagtac cagtggcgaa
     6721 acgtttgtaa ccttctgcta tcccgctgac caccgcctga tagtcggttt tcatttcata
     6781 cggcgggtcg ataaggatta aaccacggcg ggaaaccggc ggcagtttgg ccttaagctg
     6841 ctggaaacca tcggcttttt cgacgcgcgc acggctgtct ttctgaaatt cagaacgcag
     6901 caacgggtaa tcgctcgggt gcagttcggt cagttgcagg ctgtcttgtt cgcgcagtag
     6961 ctggcgagca atcaatggcg aaccggggta gtaacgcaac tgaccgctac ggttgaagtg
     7021 ttttaccaca ttgatgtacg cctccagttc tgcgggcaaa tcgtcctgct gccagatacg
     7081 ggcgatgcct tcgagatatt cgccggtacg ctcggcatgt tcgctgccta actgataacg
     7141 cccggcccct gcgtgggtgt cgagatagag aaacggttta tctttctctt tcagcgactc
     7201 gatgatcagg ctctgaacgg tatgtttaag gacgtcggcg tggttgccag cgtgaaagct
     7261 gtggcgataa ctgagcatgg gtaaaggtgt tccggtaagt aaaaatcggc ccgtattcgg
     7321 ggcgcacaaa agcttatcag gacagtatac cgaaaagagg ccgccgccgc gaaagcgtaa
     7381 cgtttctcat tgaaattcac tacacttaac cccatgctac acacattatg taaagcgcct
     7441 gttgagcgct tccttaacct ctttaaccag gactgcgcga atgacgaatc cgttactgac
     7501 tccctttgaa ttgcctccgt tttctaaaat tctcccggaa catgtcgttc cagccgtgac
     7561 taaggcgctg aacgactgcc gcgaaaatgt ggagcgcgta gtagcgcaag gagcaccgta
     7621 cacctgggaa aatctctgcc agccgttggc ggaagtggac gatgtgctgg ggcgtatctt
     7681 ctccccggtc agccacctga actcggtgaa aaatagcccg gaactgcgtg aagcgtacga
     7741 acaaaccctg ccgctgctgt cggaatacag cacctgggta gggcaacatg aagggctgta
     7801 taaagcgtat cgcgacctgc gcgatggcga tcattacgcc acgctgaaca cggctcagaa
     7861 aaaagcggtt gataacgcac tgcgcgactt cgaactctct ggcatcggtc tgccaataga
     7921 gaaacaacaa cgctacggtg agattgccac tcgtctttcc gagctgggca accagtacag
     7981 caacaacgtc ctcgatgcga cgatgggctg gaccaaactc gttaccgacg aagcggagct
     8041 ggcggggatg cctgaaagcg cgctggctgc ggcaaaagcc caggccgaag cgaaagagct
     8101 ggaaggctac ctgctgacgc tggatatccc aagctacctg ccggtaatga cctactgcga
     8161 caaccaggcc ttgcgtgaag agatgtatcg cgcttacagc acccgcgctt ccgatcaagg
     8221 cccgaacgcc ggtaagtggg ataacagcaa ggtgatggaa gagatcctcg ctctgcgtca
     8281 cgaactggcg caactgctgg gctttgaaaa ctacgccttt aaatcccttg ctactaaaat
     8341 ggcagaaaac ccgcagcagg tgctggattt cttaaccgat ctggcaaaac gcgcgcgtcc
     8401 gcaaggcgaa aaagagctgg cacaactgcg cgccttcgcc aaagcggaat ttggcgtcga
     8461 tgagttgcag ccgtgggata tcgcgtacta cagcgaaaaa caaaaacagc acctctacag
     8521 catcagtgac gaacagctgc gtccgtactt cccggaaaac aaagcggtta acggcctgtt
     8581 tgaagtggtg aaacgtattt acggcatcac cgctaaagag cgtaaagatg ttgatgtctg
     8641 gcatccggat gtacgtttct tcgaactgta tgacgaaaat aacgaactgc gcggcagctt
     8701 ctacctcgat ctgtatgccc gtgaaaacaa gcgcggcggg gcgtggatgg atgactgcgt
     8761 aggccagatg cgtaaagctg atggttcgct gcaaaaaccg gtcgcgtatt tgacttgtaa
     8821 cttcaaccgc ccggtaaatg gtaaaccggc gctgttcact cacgacgaag tgatcaccct
     8881 gttccacgag ttcggtcacg gcctgcacca tatgctgacc cgcatcgaaa ccgctggtgt
     8941 ttccggtatc agcggtgtgc cgtgggatgc ggtcgaactg ccgagtcagt ttatggaaaa
     9001 ctggtgctgg gagccggagg cgctggcgtt tatctccggt cactatgaaa ccggcgaacc
     9061 gctgccgaaa gagttgctgg ataaaatgct ggcggcgaag aactaccagg cggcgctgtt
     9121 tattctgcgt cagctggagt tcggcctgtt tgatttccgc cttcatgctg agttccgccc
     9181 ggatcagggg gcaaaaatcc tcgaaactct ggcagaaatc aagaaactgg ttgccgtggt
     9241 gccatctccg tcctggggcc gtttcccgca cgctttcagc catattttcg ccggtggtta
     9301 tgcggcaggt tactacagct acctgtgggc tgacgtactg gcggcagatg ctttctcgcg
     9361 ctttgaggaa gagggcattt tcaaccgtga aaccgggcag tcgttcctcg acaacattct
     9421 gagccgtggc ggttcagaag agccgatgga tctgttcaaa cgcttccgtg gtcgtgaacc
     9481 gcagctggat gcgatgctgg agcattacgg cattaagggc tgatcattca gtgaaaatct
     9541 gcttaattga tgaaacaggc gccggagacg gcgccttatc tgttctggcg gcccgctggg
     9601 ggctggagca cgatgaagac aacctgatgg cgctggtgtt aacgccggaa catctggaat
     9661 tgcgcaagcg tgatgagcca aaacttggcg gcatatttgt tgattttgtt ggcggagcga
     9721 tggcgcaccg acgcaaattc ggcggtggtc gcggtgaggc ggtggcgaaa gcggtgggca
     9781 ttaaaggcga ttatttgccg gatgtggtgg acgccaccgc agggctgggg cgcgatgcct
     9841 ttgtgctggc ttcagtcggt tgccgcgtgc ggatgctgga gcgtaatcca gtcgttgccg
     9901 cattgctcga cgacggcctg gcgcgtggtt atgcggatgc ggaaatcggc ggctggttgc
     9961 aggagcgttt gcagttaatt cacgcctcca gcctgacggc gctaaccgat attaccccac
    10021 gcccgcaggt ggtctatctc gacccgatgt tcccgcataa gcagaaaagc gcgctggtga
    10081 aaaaagagat gcgtgtcttt cagtcgctgg tggggccgga tcttgatgcc gatggattgc
    10141 tggagcctgc acgcttgctg gcgaccaaac gcgtggtagt aaagcgcccg gactacgcgc
    10201 caccgctagc gaatgtcgcc acgccaaacg cggttgtgac taaagggcat cgctttgata
    10261 tctatgcagg tacgcccgtg taaaagtaaa gcagggtagc gataacgcta ccctgctttc
    10321 tgcaagaata attaatggct ttccggcgtc gcaatcatgc gtttcagcca cggcaccatc
    10381 aacagcatca ctactgcaac gcccagtgtg accagaccaa tcttaccaaa cacgttggta
    10441 tagacgggca acgtctcaag cggatcggta atgttgtccg gcaccgcggt aaatgttgcc
    10501 acatagccgc ccagcaagaa cgcggcagcc tgcgtcagga accacatccc gagaataaag
    10561 cccatcaaat gctgcggcac cagggcagca atcatcgcca ggccaagggc gctaataaac
    10621 agttcaccta agctctggaa taagtacacc agcacgataa accatggcga tgtcagcccc
    10681 tgggcatccg caaaccacat tcccgcagct gcagccgtca aaaagcccag agagcacata
    10741 aacatgccga gagtaaattt catcggcatc gagaggtctt tgcctttgtt acccagatgc
    10801 gtgtaaatgc ctgccagtat tgggctggcg agtaccaccc agaacgggtt aagcgcctgg
    10861 aagctgaccg ggttgatgga aaaaccgaga atttcatgat gcacgttgtt gatggcaaag
    10921 aagttcagcg atgttggcat ctgggcgtag agaatgtaaa acaccaccgc ttcgagcatc
    10981 aggacaaagg cgacaaacat tttattgcgc ccggttttat ccagcttgaa tgcctgacga
    11041 aagaagatga tggtgacgac gatggagaga acaatcagca ccagattggc gacttctacg
    11101 ttgtgcatca gccatgcaca tacgaagatc atcaccacgc tgccaagtaa tacgtacaac
    11161 agtttgctga agctcatcgg cttgaagtcg ggttcagaac caatgtcttt caccattcca
    11221 cgacaggcga tgtaaaccag taatgcgata attaaccccg ccccgcacag gttgtaggtg
    11281 actgaataac cgaatctatc agcgatcaca ggggccagcg ataacgctat caacgagccg
    11341 atattgatcg acatatagaa cagggtgaat gcgccatcaa gccgcggatc tttcggcgga
    11401 tagcacttcg aaagcaagct ggctgggtta gctttaaaca ggccgttacc gacagcgata
    11461 gtccccaggg cgatgaaaat caggtcaggc ttaagtagcg acatgccggt catgaagtag
    11521 ccaatcgcca gcacaagtgc tccgagaaca atggtgcgtt tggtccccag caggtggtcg
    11581 ccgacatagc cgccaatgga aatgaggcca tagaccagcg cagcaaaagc accaaaagtg
    11641 acaaaagcct gctcttgcga gaatccaagc tgtttaacga agaaaaccgc cagtacgccc
    11701 tgcacgccgt agtagccgaa tcgctcccat aactcgacaa aaaagatcat gaaaaatggg
    11761 cgaggttgct gcagcatccc catgggtgtt gttgtattca tatttattaa ccttccaata
    11821 ccatcctgaa aagtatgaac gcgtaaggga taacgcgcga aaaagtttac atctgtcaga
    11881 gattcttaat attgatgcgg tgaatagatt aatacactat tacaacagaa aataaccagc
    11941 tgatcacact aatgatggaa aatatgatgg tgttaaatgt tgtattggca ggggattttg
    12001 attgcgtacg cagatcgtag gccagataag gggtttacgc tgcatcaggt aacaaggcgc
    12061 gccaaaaagc aaaacgcccg catatgcggg cgttcaggaa cacgtcgtgg ggggatgatt
    12121 attcttcttc gtcgcgcagc ggaacaatca gcatatcaac gtgaacggtg ttgatcagct
    12181 gacgtgcgga agacattagt ttgctccaga agtcctggtg gtgaccacaa accaccagat
    12241 ccatatcgta tttcttgatt gcatcgacca gaacctggcc caggtcgccg ctgccgctca
    12301 gggtttcagt gattgggtag cctgcattag tggaaagctc ggtcagcgca tgatgtgtct
    12361 cttcagagat gcgtttctgc atatcaccca gattcacatc aataagcccg gtgtataggt
    12421 cagagtagtt tacatctacg tggatcagag aaactttcgc attgtagggg cgagccatag
    12481 agactgcttt ctctaccaga actttgcttt ccggggagag gtcgaccgcg atgagaatgt
    12541 gtttataagc catagtgtta ctccttccat aaagttgtcg atgactggcc agctagcgtt
    12601 tcttgtgccg ttacgtcacc cgcgtcctgc gaactacgtg cgcggggcta acccagccgt
    12661 aaagattcag tgttaagacc atccttacct tatagcgacc cggatgatcc gtcaatccgc
    12721 cttgcttact cgttagataa acaaattttc gacatgaata tattgatagt ggttaacctt
    12781 ctggaaaaaa aacaacctga tctcctacac tatctataga gccgctcgga tgttggtcac
    12841 cacaaaagcg gtttctccag gacctggtgt taactgattg gcagcgtatc gggagcggta
    12901 gccccggggt ggaactccgc gggcaggtcg ccggggagga ggtatgataa gcaccgtcgc
    12961 attattttgg gctttatgtg tcgtttgcat tgttaacatg gcgcgctatt tctcatcact
    13021 acgcgcgttg ttagtggtac tgcgtaactg cgatccattg ctctatcaat atgttgatgg
    13081 agggggcttt tttacctcac atggccaacc caacaaacag gtgcgtctcg tttggtatat
    13141 ctatgcccaa cgttatcgcg atcatcacga tgatgagttt attcgccgct gtgagcgggt
    13201 gcgtcggcag tttattctga ctagcgcatt gtgtggtctg gtggtggtca gcctgattgc
    13261 attgatgatt tggcattaag cacttcagca ataataacgc ataaaaaaag cgggccagta
    13321 aactgacccg ctcgttttgg tgcgtacgat tacaggaact gcaaggagag ccagtacagc
    13381 ccgccggaaa gcagtaccgc agccggaagg gtaaacaccc aggccatcag aatactggtt
    13441 acggttttac gctgtaagcc gccgccatct accaccatcg tccccgcgac agaagaggag
    13501 agtacgtgag tagtggaaac cggcatcccg gtataactcg ccaggccgat agacactgcc
    13561 gccgtcatct gggcagacat cccctgagcg taggtcatgc ctttcttacc gattttctca
    13621 ccgatagtcg ttgccacacg gcgccagcca atcatcgtac cgatacctaa cgccagcgcg
    13681 accgccatga tgatccacac cggtgcatac tcgatggtgc taagcatgtc ggacttcagt
    13741 ttcttcaaca ggcgctgatc gtcagcactc acgccaggca tcttcaccac tttgtcgata
    13801 gtgtcagaaa cgcacagcat aatgcggcgc atctggctac gttgatcaag cgacagcttg
    13861 tcgtagcttt ccacatcggt ggtcaacata cctttcaggc ggttgagcgc gttaatggta
    13921 ttcgacggat ggcagtggaa ctccgcaggt tgcgttgcgc cagcttccgg agccggtact
    13981 aactgatcag caccggtagc ctgtttgagc agcgcaggat gctgctcaaa gtaagcttcg
    14041 acgttgttga tggcatcacg ggtacgggtg atttcgtagc cagtggcatt catgttcacc
    14101 acgaagcctg ctggcgcgac gccaatcaat accaacataa ccagaccaat gcctttctga
    14161 ccatcgttcg cgccgtgcga aaacgccacg ccgatagcgg aaaggatcag cgcgatacgc
    14221 gtccagaacg gcggcttttt cttgccgtct ttcttttcac gctccgctgg ggtcaggtgg
    14281 atacgggcgc gtttcttggt gccgctccag tagcgacgca gcaagaaaat cagaccgcca
    14341 gcaaacacca ggccgacaat aggggaaacg atcagagaac cgaaaatact taatactttc
    14401 gggatattga gtgcatccac cactgacgtc ccggtcatca acgcattggt taaaccaatc
    14461 ccgatgatcg cgccaatcag cgtatgagag ctggatgcag gtaaaccaaa gtaccaggta
    14521 cccaggttcc agataatcgc cgccagcaac atagagaaca ccatggcaag gccatgagac
    14581 gatcccatat taagcagcag atccgtcggc agcatatgca caatggcata ggcaacactc
    14641 agaccaccca gcaaaacacc caaaaagttg aacaccgccg ccataaccac ggcgagctga
    14701 gaacgcatcg cgcgggtata gataacggtt gccacggcgt tggctgtgtc atggaaacca
    14761 ttgatggctt cgtagaacag cacaaaagcc agtgcaagca ataataacag cccggtatgc
    14821 aaatccaggc cagcaaacaa atgtagcata ggacgttacg ccattttgag gacatgaacg
    14881 cggcgcatta tcagtgactt tcgcggcgcg ggcaaagtga aatatagact ttttttgatt
    14941 tgcctcctgt atggatttca ctcaaaaaat aattatctta tataattcag gcaaatactt
    15001 ccttttagta atattgatgc tggtgcgacc actgaggaat ctttacaatt cacgcccgtt
    15061 ttttctaaga ggagcgcaac gtggaaaggt ttgatgccat tattataggc gctggtgcgg
    15121 cgggtatgtt ctgttctgcg ctggcaggtc aggcaggacg ccgggttctg ctgatcgata
    15181 atggtaaaaa accagggcgc aaaatcctta tgtctggcgg tgggcgctgc aactttacca
    15241 acctttatgt cgaaccaggc gcttatctga gccagaatcc gcatttttgt aagtctgcac
    15301 tcgcgcgttt tacccagtgg gatttcattg atctggtcaa taaacacggc atcgcctggc
    15361 acgagaaaac gttaggacaa ctcttctgcg atgactccgc gcagcagatt gtcgacatgc
    15421 tggtggatga gtgcgagaag ggcaatgtaa ccttcagatt gcgtagcgaa gtgctgagtg
    15481 tggcgaagga tgaaacaggc ttcacgcttg aactgaacgg catgactgtc ggttgcgaaa
    15541 agctggtcat cgcgaccggt gggctgtcaa tgccggggct gggcgcgtcg ccgtttggtt
    15601 ataagattgc cgaacaattt ggcctcaacg tgctgccgac ccgcgcgggt ctggtgccat
    15661 tcactctgca taaaccgttg ctcgaagagt tacaggagct ggcgggcgtg gcggtgcctt
    15721 ccgtgattac cgctgaaaac ggcatcgttt tccgtgagaa cttactcttc acccatcgcg
    15781 gcttgtctgg accggcggtg ttgcagattt ccagctactg gcaaccgggt gaatttgtca
    15841 gtatcaatct gctaccggat gtggatctcg aaaccttcct gaatgagcag cgtaacgcac
    15901 atccgaatca gagcctgaaa aacacactgg cggttcatct accgaagcgg ttggttgaac
    15961 gcttacagca actcgggcaa atcccggatg tttcgctaaa acagctcaac gtgcgtgacc
    16021 aacaggcact gattagcaca ttgaccgact ggcgcgtaca acccaacggc actgaaggct
    16081 atcgcactgc cgaagtgacg ctcggcggcg tggacaccaa cgaactctct tcacggacga
    16141 tggaagcgcg caaagtgcct gggctgtact tcatcggcga agtgatggac gttaccggct
    16201 ggctgggggg ctataacttc cagtgggcgt ggtcgagtgc gtgggcttgt gcgcaggatt
    16261 tgattgctgt tcaaaaacaa taaagttgtg ccctaagcac taaagttgta accactctca
    16321 aaaaatgacg ctaatgtttt atacttacta tatgtgtggt tatagggttg tagactatta
    16381 aacactcatg caggtacgta tcaatgatgt tttgtgggta cgatcatggc taggggcagg
    16441 cgtcttaaat cctatcagga ttatgaaaat gcgctaggtg acggcatagg gttgggctat
    16501 gggcaaagtt atcaaccctg gcttagagct caggacgtta gatcccatgg aaaccgttcg
    16561 atagtctttg gccttaagac gtttcggaat caccatttct tttcttctgt cgaaagtaac
    16621 tttttctact tggctgagtt taatgacttg gtgatcgata tccgcgaaca attcccactc
    16681 atacctctac tgctcaccca tcagatagcc aatcacttag atgtgcagca cccgcttgtc
    16741 aggggagtca gaggcgtacc agccgatgtc ctgaatgtta tgacaactga tttcttgctg
    16801 accctgagaa tgccgggagg ggggcttcgg tataaagcaa ttgcagtaaa gcaaaacgaa
    16861 agcataccta aacgcgaagc agaaaagctt gagatagaga gaatgttttg gcaactgatt
    16921 gatgttgagt tccaaatcta tactggttcg gaactgaatc atgtcacggg gcagaacata
    16981 tgttgggcta cctcggtgct gagaaatggg tctgagtttt ttgataaata tccgcttgat
    17041 aaaattttat ggaggcttaa gccggatgtt tatccgattg tggaactacg cgcgatgatc
    17101 tcatcaatct ttgatgtcga tgaacaagaa gcaatcatgt tattgcaggc aattattggg
    17161 ttaaaaatga taaatgtgga tctatcttat ccaattctcg aaacaggtct gataaaaata
    17221 atttccaatg gttactgcat agggctgaac gcaaatggat attattagaa attcagtatg
    17281 gcaatctcac gacagtgacg tgctatctga tgggctttat cgcgttctgg attttgacag
    17341 agataccgat ttgttgattt tatttgaaat aaaaaaagaa agaacgagta aaccaattcc
    17401 attctcactt tcacaattta catatggtgt ggactcgcaa agaataaccc ataagaaata
    17461 tacaccccca tcatatatgt tgattgatga gagaaaaata aatgatgaag acagaaagcg
    17521 acgggatgta aactataaga ttattaaaga acttgtagag gataaaatat tcgtatttga
    17581 ttatgcgatt cataaaaaat cacatttgtt aacagagtac tccagaaaaa aacaaatccc
    17641 acaacgtaca atcaggacgc ttctaactct ttactggaaa catggacagg atatatatgc
    17701 tttgctaccc gcatacccga actgcggtgc tgcaggtaag agtagaatca gacatgaagt
    17761 taagctcggg aatgcaaaga aaaacagagt attacccaat atacgttcga aagtttatat
    17821 ccttaatgaa ggggatattg aaaacatcaa aaagtcccta actaggtttc attacaaaac
    17881 tcatggggaa acgataaaga aaacgctggt aagacacatt gaattatatt tcaaggatga
    17941 aataaagaaa gcaaacgctg aaaacagggc tccctacatt ccttctttaa aacaatttac
    18001 ttactggaat agaaagttgt tttcaaaaga gttctctata aataagaaaa actctaaaaa
    18061 agaaatagcc ctcaagatgc gtggccttct ggggagtgcc gctaatacga ccgtattgcc
    18121 gggtgatgtt tttgaaatag actcaactgt agctgatgtg caccttgttt caagcttaaa
    18181 caggcgtaaa gttattggcc gaccaactat ttatacagta gtggatcgtg cttcaaggat
    18241 gattgttggt cttcatgttt ctttgtatca tgcttcctgg cgagcggccc ggcaagcgtt
    18301 ggcaaattgc tttatgccaa aaaaagaata ctgtagaata tttggtgttt ctattaatga
    18361 agatgaatgg ccttgttctc atataccatt aacacttatg tgcgataatg gagagatgat
    18421 tggtcttaaa cctcaagata agatgactcc tctgactaaa cttgagtttg caccggttgg
    18481 cagaggggac agaaagagta ttgttgagcg ttgtttcggg attttaaatg atgaagctat
    18541 tcatgagctt acaggagcaa cgcgaagagg caaaattgtt aaaggagaac caacaccgca
    18601 atccagagcc tgtttaacga ttcaggaggt cacctcagtg ctgattcggg aaatccttgc
    18661 acataatcaa agaacgtatg aggagttggc ttatattaat ccattgttga ttgaacatga
    18721 tctcattatt tcgccaaaaa acagctggat gattagcgta aagcaaggac ggttcagcgg
    18781 aagagtcgtt acagcagatg aagtcattgc tcgtttgtta atccccgtga cagcaaatat
    18841 tacagccggt ggaattcagt ataataattt attttatgaa tgcgatcctg acattgcttc
    18901 agcagcaaga gtattcggac gaacttcctg tgaagcaaga attgatgata attgcgtaga
    18961 ttatttgtac gtaagattcg ataaaaatag tgtcttcaga aagcattatc tactcaaaaa
    19021 aagagatgta tttaaaggta aagctcatct tgatacagat gttatggccg actgggtaga
    19081 tacgcaaaaa gaaataaatg cattcacact gaatgttttt aacaatagta aagttaagga
    19141 taaaatcaac aggcaaggta ttgaacgcct gaacgatatt tctgatcccc gccgggtgca
    19201 tggtaaagat atcaggcgga acagaaaagc tgaactggac ctattagggc gtacaaatct
    19261 gcctaatgaa aaaaatgaac ctgtacttcc ttcatccagt aatattctgc ttttgcctgg
    19321 ccgtgaagaa aaacagcgat ggttaaacgc taagaaaaaa caagaggacg cagaaaaatg
    19381 aatatgttac ggagcgtctc agcaatttat aaagagccgg gcttgcctga atatcggggc
    19441 aatcccttaa ttgaggcact tcctgaagcg ctttccgatg atgaagtgtt ttttgaaatg
    19501 agttactttc ctgaaattga cgaagatatt cgctggactg ctcctgctaa aatcagggag
    19561 cagtatgtcg atcggataaa gaaatttcga tgtccacaaa cgaatcttat cgaagcttac
    19621 agaatgattt accgggcttt gagggaaagt tatgctgccc gaaatccttt aaaaagtggt
    19681 actgttcagt acctgcatta ctatggtaac gagcgacctg acatagagcc agatagcggg
    19741 tattttaagt ctcaggctga aactattacg atagtcggga tgagtggctc cggaaaaacc
    19801 accatgattg agcaaattat ggactacttt ccccaggtca ttgagcacag tagttataaa
    19861 ggtgcttttc ccgggctaag caagcaaatc gtgtggttaa agattaattg tccgtacaac
    19921 tccagtgtca gagacctttg cgaagaaatt cttctaaagt tagatgatgc tagcggcatc
    19981 gaacgaacta caccagagat caggaatggt cctctggcga ggcagatagc gcagagaatt
    20041 aaatcatcgt ttttgggtat tcttgttatt gatgaaatgc agcggctgaa attttccaga
    20101 acgggcggtg aaaacaaact cattgatttt cttcatgaaa ttgtagattc aatgggtgta
    20161 tcaatggttt tttgtggaaa cccccctttt gatgaaacac tggccagaaa aatgcgtatt
    20221 gccagacgag cggaatcggg tggttatatg aatattctca atgtcaggta tgactcacag
    20281 gactggcagt cgtttattca ttatttatgg ccattacaat ggacaaatat tgaaactccg
    20341 ttaagtgatg agcttaatga aaaacttttc gttttatcta aaggtaacat cggactggct
    20401 cagatgattt atcgcagggc acaattaaaa gttattggaa gcggcaatga actgatcagt
    20461 agtgtagtgt tgtcgtcttc tgtaccggtg ctcgccagtc atacggagga gtatcaggag
    20521 gctttgatac ttggagctgt gggtgggtat gatgataaat attgctctgt atctcatgca
    20581 ggcgaagcct caacggattt tactgccgac aggcacgcga aggctctgat tcctggagat
    20641 attgacaggc cacagcataa agaatttgca caaaaaattg atgaggctat gcaaaatttc
    20701 gaaagtaaaa tcatatcttt cgatcgggag cgtattatca gcagggctgc tgataacaac
    20761 gaaacctact catcgcttca gcgttgcggt ctgttaaata cagacccact taatgaattg
    20821 taatactgaa tttattgttc agagtggttg ctgagaggtg ggcgaatgat tctacttcct
    20881 gttttacctg atgaaacctt gtttagccgt atatgtcggt cagttactgt gtatggtatg
    20941 tctccttcta ccatgttaca aatccttttt ggaaaatctg atgttagcgt acacccaata
    21001 ctcagttccg gattaaacga tatttctctt catacgtcag agagtgcaga gcaactcttg
    21061 tatgaacaga cattgctccc tcttttttcc tgggccctgc catccagccg caaagaaatt
    21121 atggatttga gaacgacacc gggcagacta aatcggttgt gccgtcttag taatttctcc
    21181 tccgggctac agactgtttt aaaattttgt ccggtttgtg cacgtgaaga tgcgtaccat
    21241 catggtgtta catactggca tcttcttcat caacttcggg ggattacgac ctgctatcga
    21301 catcagatat tacttgaaag catctgtgtt cctccttcgc cacatattcg caaagggctg
    21361 atgcccccgg tttcatatat tgaacatctg agtactgagt ttgaattcga ttttgctaaa
    21421 ttttgctacg aatttataaa tatgatccga aggggagatg caaatcatcc tgattacatg
    21481 gatatactga aacaacttaa tttattatca gtatcaggaa atataaaaaa aggggttttt
    21541 tattcacatg tttatgcgaa atgtaggtta ttagattcgg gttcttcagc aattttgcct
    21601 gaatccattt cggattatca ctactgggag cctttactca ggaatcaatc atgccagcat
    21661 ccaacaaagc atctgttact gtgctattgt ttgttaagaa taacatggcc agtacatgcc
    21721 gatatccata aaaaatcaaa gagcatcgct tttgatgatg atacagccca gttttgcctc
    21781 ctgagtgaaa aacataacag tattaattct cttgctaagg aacttaacaa gagtagatgt
    21841 tttattaaag gagtaatcca taaaaagtat gtaaagaatt ataagtgtag cattaaaata
    21901 aacatattaa ctgagttcct tattaaatcc atggctgtga gagggtttag tttagcatca
    21961 atagcaaaga aaaactcctt atcggaaggg agtgtttcat cggtgatttc ttcattttat
    22021 ggattatgtt catggcgtaa aaaatgtaaa agagactctt taagacgacg tcataaacaa
    22081 aaaatcctgc ggttcatgca tggcctcact ggtcctgtga ctcgaaaagt agtcagggcg
    22141 aattgttatg caagtttttt ctggctaaac agatttgaaa gccaatggct tgatttttat
    22201 ttaccaaagg aatcacggcg ctgtaatgtt aaaagggtca attgggatga aagggataag
    22261 ttttcattat cattaataaa aaatttttta gctcaggggc agtgctcact gtcactgtca
    22321 agacttgatt ctgtactggg gggacatggc tggttattaa aaaacagaga taagcttcca
    22381 gttactatgg ctttgattaa tatgagtaaa tctaagtagt tgaatgaggt tgatatgata
    22441 tatgaaaatg aattgtggca cagttttctt ctacgtactc agattatata taatatacaa
    22501 aattacagga atattatctc agcgcatggt gcattaagat atgatgctta cccacgaccg
    22561 gagttgatta atgtgtataa gctacacaga actcaggact tatatgatat tcttaccaca
    22621 gtaaatggat ttatactcac ttccagaatg atttactctc catatggagt ttacagctat
    22681 tttgatgcta tggaagatgc tcacaataaa ggatttaata ttataaatac atatcctgtg
    22741 gtaaacatct cacgaattcg ctattgccac agttgtattc tggaagatat tcgtatcaaa
    22801 ggtgttggct atcttcgaca tagatggcag tttgaatcaa gatgttgcca tcacaatcaa
    22861 agtttatacg aagtttgctt tgataattat tctggcgcgg ttaaaggact ctgtgagtta
    22921 atggtgagtg ggttaaatcc ttcggggtgt tattcacttg tagttgatgt ttttaaccca
    22981 tttcagactc cggtatatct attgccatgt tctgatggca gagtcagaac gtggattctg
    23041 gaaaataagg atgagttggt taattttctg tgtagtattt ttaaaaataa atcttcttca
    23101 ggtctgaaac gtgtaattga tgtaaagata atg
//
LOCUS       DAZGXZ010000078        23075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000078 DAZGXZ010000000
VERSION     DAZGXZ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.078"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..578
                     /gene="ybeT"
                     /locus_tag="ACHKJF_003149"
     CDS             24..578
                     /gene="ybeT"
                     /locus_tag="ACHKJF_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0950462.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPFTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGSGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(588..2015)
                     /gene="djlB"
                     /locus_tag="ACHKJF_003150"
     CDS             complement(588..2015)
                     /gene="djlB"
                     /locus_tag="ACHKJF_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP0950463.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2012..2719)
                     /gene="ybeR"
                     /locus_tag="ACHKJF_003151"
     CDS             complement(2012..2719)
                     /gene="ybeR"
                     /locus_tag="ACHKJF_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0950464.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2883..3860
                     /gene="ybeQ"
                     /locus_tag="ACHKJF_003152"
     CDS             2883..3860
                     /gene="ybeQ"
                     /locus_tag="ACHKJF_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFP0950465.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3930..4412)
                     /gene="ybeL"
                     /locus_tag="ACHKJF_003153"
     CDS             complement(3930..4412)
                     /gene="ybeL"
                     /locus_tag="ACHKJF_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP0950466.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4647..7229
                     /gene="leuS"
                     /locus_tag="ACHKJF_003154"
     CDS             4647..7229
                     /gene="leuS"
                     /locus_tag="ACHKJF_003154"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP0950467.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7244..7825
                     /gene="lptE"
                     /locus_tag="ACHKJF_003155"
     CDS             7244..7825
                     /gene="lptE"
                     /locus_tag="ACHKJF_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP0950468.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7825..8856
                     /gene="holA"
                     /locus_tag="ACHKJF_003156"
     CDS             7825..8856
                     /gene="holA"
                     /locus_tag="ACHKJF_003156"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP0950469.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDVVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8858..9499
                     /gene="nadD"
                     /locus_tag="ACHKJF_003157"
     CDS             8858..9499
                     /gene="nadD"
                     /locus_tag="ACHKJF_003157"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP0950470.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9523..10134
                     /gene="cobC"
                     /locus_tag="ACHKJF_003158"
     CDS             9523..10134
                     /gene="cobC"
                     /locus_tag="ACHKJF_003158"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP0950471.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10394..10711
                     /gene="rsfS"
                     /locus_tag="ACHKJF_003159"
     CDS             10394..10711
                     /gene="rsfS"
                     /locus_tag="ACHKJF_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP0950472.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10715..11182
                     /gene="rlmH"
                     /locus_tag="ACHKJF_003160"
     CDS             10715..11182
                     /gene="rlmH"
                     /locus_tag="ACHKJF_003160"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP0950473.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11213..13114
                     /gene="mrdA"
                     /locus_tag="ACHKJF_003161"
     CDS             11213..13114
                     /gene="mrdA"
                     /locus_tag="ACHKJF_003161"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP0950474.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13117..14229
                     /gene="mrdB"
                     /locus_tag="ACHKJF_003162"
                     /gene_synonym="rodA"
     CDS             13117..14229
                     /gene="mrdB"
                     /locus_tag="ACHKJF_003162"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP0950475.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14240..15328
                     /gene="rlpA"
                     /locus_tag="ACHKJF_003163"
     CDS             14240..15328
                     /gene="rlpA"
                     /locus_tag="ACHKJF_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP0950476.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGASSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLESSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15468..16679
                     /gene="dacA"
                     /locus_tag="ACHKJF_003164"
     CDS             15468..16679
                     /gene="dacA"
                     /locus_tag="ACHKJF_003164"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP0950477.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16790..17053
                     /gene="ybeD"
                     /locus_tag="ACHKJF_003165"
     CDS             16790..17053
                     /gene="ybeD"
                     /locus_tag="ACHKJF_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP0950478.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17154..17795
                     /gene="lipB"
                     /locus_tag="ACHKJF_003166"
     CDS             17154..17795
                     /gene="lipB"
                     /locus_tag="ACHKJF_003166"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP0950479.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18054..19007
                     /gene="ybeF"
                     /locus_tag="ACHKJF_003167"
     CDS             18054..19007
                     /gene="ybeF"
                     /locus_tag="ACHKJF_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP0950480.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19216..20181
                     /gene="lipA"
                     /locus_tag="ACHKJF_003168"
     CDS             19216..20181
                     /gene="lipA"
                     /locus_tag="ACHKJF_003168"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP0950481.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20282..20485)
                     /gene="tatE"
                     /locus_tag="ACHKJF_003169"
     CDS             complement(20282..20485)
                     /gene="tatE"
                     /locus_tag="ACHKJF_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP0950482.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20614..21402)
                     /locus_tag="ACHKJF_003170"
     CDS             complement(20614..21402)
                     /locus_tag="ACHKJF_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP0950483.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21495..21878
                     /gene="crcB"
                     /locus_tag="ACHKJF_003171"
     CDS             21495..21878
                     /gene="crcB"
                     /locus_tag="ACHKJF_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP0950484.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21933..22142)
                     /gene="cspE"
                     /locus_tag="ACHKJF_003172"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21933..22142)
                     /gene="cspE"
                     /locus_tag="ACHKJF_003172"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0950485.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22317..22877)
                     /gene="pagP"
                     /locus_tag="ACHKJF_003173"
     CDS             complement(22317..22877)
                     /gene="pagP"
                     /locus_tag="ACHKJF_003173"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP0950486.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5950 a         5780 c         5693 g         5652 t
ORIGIN      
        1 aaagaaaatc gaagcattca cacatgaata aaaaattaat gtatatattc gcaattttta
       61 tagttgcagc aattacctgt attagccaac ccaagaaaac gacgttgcgt gataaagcca
      121 tggtgaatta tgcctttgat tatttaagat caccgggcag tcttccattc accacggcag
      181 ccacggagct ttccgcgatt catggtcact caacgtcgca atatcgcctt ggagaatttt
      241 atcttcatgg tagcgacggt aaaccactgg attatacaca ggcgagatac tggtatgagc
      301 aatcagcgga acaggaaaat ccacgcgcgc aaagtaaact ggggtggatc tacctcaaag
      361 gtctgggggt caaacccgac acccgtaaag caattctctg gtataaggaa gcagctgaac
      421 aagggtatgc tcatgctcaa tatactttag gtttgatcta cagaaatggc tcaggtatta
      481 atgttaacca ttatgaatct caaaaatggt taaaactggc cgccaaacaa cattacaaaa
      541 atgcggaaag attacttgcc gggcttcccg cacattaacg tcgactgtca cccgaacaga
      601 tggagtattt tccctatcaa accagcaaag ataaaaatat agaaaataat tttaatgatg
      661 cccagacagc ctggcccacc atcgtcaacg ggcattggct gcggttgcgg ctccggttca
      721 acagtttgct tctcggtaaa attcgccgcc tgaattaata atcgacgaat aattaatgct
      781 tccagtggac aacgatcttc cgcatcaagg cggctcacaa tcggcattaa tgattcttct
      841 gtcggttggc tagcctgcac taattgtttg gcaactaaat ataatggcca taagggatgt
      901 tctgtatcac gcgtgagtga gatccgcggc ttacgtcctt tgtgcaaaac catctcgacc
      961 agtgaacgcc ccagcgcaga gagtttttca tcgctgagta tttcgtataa atgaaaacgc
     1021 gtacgggcgc tgtcgccggg gacataaagt agctgtttac cctgatcgtt aaccagttca
     1081 cgagcttcaa tcatcatcac cactaacggg taataatcga cccgatgctg acggcaccat
     1141 tgcaatatta aagcatccgc ctgggtggag ggatactcgc gccagtagtc acaaagttct
     1201 gccagcaagc tttcgccttc tccacaatag acgcgttgcg cgtattcata ataacgcgca
     1261 tcttcattat caggttcagc ttcccgccac gaccgggcgt aatccagaag ttcggggata
     1321 tccaggcgac ctgcgctata ccagcggagt aaatttctgt gcagttttgc atcatcgggg
     1381 atgatcactg cgcgatgttg cgctaaatac aatgtcagcc attctggatg cgatgaccaa
     1441 atccgagcga acatttggta aaaatcgatg gtgtcatttt gcacggcaac gggcagatgc
     1501 agaatggtct ggaagttaaa aacattcccc ttcgcaatgg catataaaaa ggattccagt
     1561 tcttcttcgt ctatttcccc gacgttttcc tcctgcaacc accgcaatct ttctgctaat
     1621 aacaccacgc actcgaagga taaatgggca gtgttcatgg ctattgtgca cagcgaccag
     1681 cgtaattcat caatatcttc catcgagcaa taatttaatt gctgaatgaa tcgctgccag
     1741 gcagagggca gaaaacgttc gctatcagag gcaagtaacg cacgaaaggc gagcagaatt
     1801 tcatgttctg ctacctcata ttcttctggt tgccaaacag atttagcagg cgactgcgca
     1861 atccgtagcg cctcctcata cgcctgacga agttgtttaa aaccctgcgg atcggtttct
     1921 ggatgaaagg acggtaacag cgccagataa gcgcggcgga taatatcgac gtcagtcgtt
     1981 tcctctattt cgagaatctt ccagcaattt ttcataggtc gctcatatcc ttaagcgttt
     2041 ccgggcatgc agaatagtaa gccaatggtt gccaggacaa ggtagggtaa tacgcatcat
     2101 cactggcaaa taattgataa aacatatcat tgccggaatc gtcgtattct ttacgcgcta
     2161 agaattcgcg taataactgc agatgatcat cttcgggaga ctgccaatat tgccgaccaa
     2221 aggtataacc ggcaaagtat tgccgccagt tacgataata acgtagagcc cgtagatgaa
     2281 tgcgcgactg gatccataga ctttcttctt cgctcaacca gttattaagt acgcccattc
     2341 ggcttaaaaa acccatccgg acataatccc aggctttaat tcctccctca ccacagcatt
     2401 cggcggtgct ggcgacaaat tgcgcataag cctgaccttg ctcatttagt tcagcaagat
     2461 agtcacgcca ttcacatggc gaataacgaa accagcggtg ataaaaccct gccaaccgag
     2521 cggcatgacc gttatcgatc attcggtgaa ttgtgcgcag caggtcatca cgggcatcaa
     2581 ttccccagga ggaagaaagg tcaaaaaagc atgtacctaa atacattttg ggtgatgaat
     2641 gcgaaggtaa acagcattca ttgcgtattt ccatcggagt ggaaagtgca aacaatattt
     2701 tttgcgactc catgtccata taatttatcc ttaaatattc attaaatgaa tattagccgc
     2761 attgcgaggc tcgtttattt atacttttct gacgcgacat aatacgcata aaataaggct
     2821 tacgcaaaaa tggaacacat aaaaatataa tatgttttat ctgcaaccat aaggaaatga
     2881 taatgatttt cacgtcaagt tgctgcgata atttatcaat agatgagatt atcgaacgtg
     2941 ctgaaaaagg cgattgcgag gctcaatata ttgttgggtt ttattataat cgcgatagcg
     3001 caattgattc tccggacgac gaaaaagcct tttactggct gaagctggcc gctgagcaag
     3061 gtcattgtga agcacagtat tccttagggc aaaagtatac cgaggataaa agccgtcata
     3121 aagataatga gcaagccatc ttctggctga aaaaagctgc cctacaaggc catactttcg
     3181 cttccaacgc ccttggctgg acactggatc gtggagaagc ccccaattat aaagaagcgg
     3241 ttgtctggta tcagatagcc gcggagagcg gaatgtctta tgcgcaaaat aatcttgggt
     3301 ggatgtacag aaatggcaac ggagtcgcaa aagactatgc gctggcattt ttctggtaca
     3361 aacaagctgc attacaaggc catagtgacg cgcaaaacaa tctggccgat ctttatgaag
     3421 acggaaaagg cgttgctcaa aacaagacac tcgccgcatt ctggtatttg aaaagcgcac
     3481 agcagggtaa tcggcacgcc cagtttcaaa ttgcatggga ttataacgct ggcgaagggg
     3541 tggaccagga ctataagcaa gcgatgtact ggtatctgaa ggctgccgct caggaaagcg
     3601 tcggcgctta cgtcaacatc ggttatatgt ataaacacgg acaaggcgtt gagaaagatt
     3661 atcaggctgc ctttgaatgg tttacgaaag ccgctgaatg caatgacgcc actgcctggt
     3721 ataacctggc cattatgtat cactacggag aaggaagacc tgtcgatctc cgacaggctc
     3781 tcgacctgta tcgtaaagtt cagtcatccg gaaccagaga tgtcagtcag gaaattcgtg
     3841 agactgaaga tttactgtag agataaaaac aagggccggg gaatttcatt cggcccatat
     3901 tctctttatt gctgtgcgaa atcagactat tacggctcaa acgggcgtct ctggaactgg
     3961 tcatgaccac atttcgggca tagcgtcagc acttccggtg tgtagatcgg gagatggaag
     4021 tgacatttct cgcagaccag atttcccagc ccgaccactt ctccgctgtg ataaacccca
     4081 tgatgattga ggtcctggaa aacttcgcgc cattcaagct gcgttttatc ggtgatatct
     4141 gccagctcct gccataagct ttctttaatc acccgcataa agacgctgtc agattcttct
     4201 ttcaggctct cttcatagct catggcgaac tcttccaggt cacgtcttac agctcgcgtc
     4261 agctcatcga cctcggttcg cgttaactcc cctgttttta ttacgcgctc gcgcgcctgt
     4321 tccaccagtg cgtcgatatc acgttcgcca ttgcgcaggc gttcgctcag tgacgcgacc
     4381 agttcacggt aatattgagc aaccttgttc atcgtttcat ctcccgggtc gttaactgac
     4441 tataataata gacgttcttt gttccttgct ttgctaatac gcccacaaac tctgtaaatt
     4501 aatgtatgaa taatccgggc atcatttcaa cgtcgagtgc gcgaaaggct gttttgacgc
     4561 ggacgtttgg gctatgctat gcggatctga aaaaccacct caacgctaca tttgtagccg
     4621 tattgaaaac aggaccactg gctgccatgc aagagcaata ccgcccggaa gagatagaat
     4681 ccaaagtaca gcttcattgg gatgagaagc gcacatttga agtaaccgaa gacgagagca
     4741 aagagaagta ttactgcctg tctatgcttc cctatccttc tggtcgacta cacatgggcc
     4801 acgtacgtaa ctacaccatc ggtgacgtga tcgcccgcta ccagcgtatg ctgggcaaaa
     4861 acgtcctgca gccgatcggc tgggacgcgt ttggtctgcc tgcggaaggc gcggcggtga
     4921 aaaacaacac cgctccggca ccgtggacgt acgacaacat cgcgtatatg aaaaaccagc
     4981 tcaaaatgct gggctttggt tatgactgga gccgcgagct ggcaacctgt acgccggaat
     5041 actaccgttg ggaacagaaa ttcttcaccg agctgtataa aaaaggcctg gtatataaga
     5101 agacttctgc ggtcaactgg tgcccgaacg accagaccgt actggcgaac gaacaagtta
     5161 tcgacggctg ctgctggcgc tgcgatacca aagttgaacg taaagagatc ccgcagtggt
     5221 ttatcaaaat cactgcttac gctgacgagc tgctcaacga tctggataaa ctggatcact
     5281 ggccagacac cgttaaaacc atgcagcgta actggatcgg tcgttccgaa ggcgtggaga
     5341 tcaccttcaa cgttaacgac tatgacaaca cgctgaccgt ttacactacc cgcccggaca
     5401 cctttatggg ttgtacctac ctggcggtag ctgcgggtca tccgctggcg cagaaagcgg
     5461 cggaaaataa tcctgaactg gcggccttta ttgacgaatg ccgtaacacc aaagttgccg
     5521 aagctgaaat ggcgacgatg gagaaaaaag gcgtcgatac tggctttaaa gctgttcacc
     5581 cattaacggg cgaagaaatt cccgtttggg cagcaaactt cgtattgatg gagtacggca
     5641 cgggcgcagt tatggcggtt ccgggtcacg accagcgcga ctacgagttt gcctctaaat
     5701 acggcctgaa catcaaacca gttatcctgg ctgctgacgg ctctgagcca gatctttctc
     5761 agcaagccct gactgaaaaa ggcgtgctgt tcaactctgg cgaattcaac ggtcttgacc
     5821 atgaagcggc cttcaacgcc atcgccgata aactgactgc gatgggcgtt ggcgagcgta
     5881 aagtgaacta ccgcctgcgc gactggggtg tttctcgtca gcgttactgg ggcgcgccga
     5941 ttccgatggt gacgctggaa gacggtaccg taatgccgac cccggacgac cagctgccgg
     6001 tgatcctgcc ggaagatgtg gtgatggacg gcattaccag cccgattaaa gcagatccgg
     6061 agtgggcaaa aactaccgtt aacggtatgc cagcgctgcg tgaaaccgac actttcgaca
     6121 cctttatgga gtcctcctgg tactatgcgc gctacacttg cccagagtac aaagaaggta
     6181 tgctggattc cgaagcggct aactactggc tgccggtgga tatctacatt ggtggtatcg
     6241 aacacgccat tatgcacctg ctctacttcc gcttcttcca caaactgatg cgtgatgcag
     6301 gcatggtgaa ctctgacgaa ccagcgaaac agttgctgtg tcagggtatg gtgctggcag
     6361 atgccttcta ctatgttggc gaaaacggcg aacgtaactg ggtttccccg gttgatgcta
     6421 tcgttgaacg tgacgagaaa ggccgtatcg tgaaagcgaa agatgcggca ggccatgaac
     6481 tggtttatac cggcatgagc aaaatgtcca agtcgaagaa caacggtatc gacccgcagg
     6541 tgatggttga acgttacggc gcggacaccg ttcgtctgtt tatgatgttt gcttctccgg
     6601 ctgatatgac tctcgaatgg caggaatccg gtgtggaagg ggctaaccgc ttcctgaaac
     6661 gtgtctggaa actggtttac gagcacacag caaaaggtga tgttgcggca ctgaacgttg
     6721 atgcgctgac tgaagatcag aaagcgctgc gtcgcgatgt gcataaaacg atcgctaaag
     6781 tgaccgatga tatcggccgt cgtcagacct tcaacaccgc aattgcggcg attatggagc
     6841 tgatgaacaa actggcgaaa gcaccaaccg atggcgagca ggatcgcgct ctgatgcagg
     6901 aagcgctgct ggccgttgtc cgtatgctta acccgttcac cccgcacatc tgcttcacgc
     6961 tgtggcagga actgaaaggc gaaggcgata tcgacaacgc gccgtggccg gttgctgacg
     7021 aaaaagcgat ggtggaagac tccacgctgg tcgtggtgca ggttaacggt aaagtccgtg
     7081 ccaaaatcac cgttccggtg gacgcaacgg aagaacaggt tcgcgaacgt gctggccagg
     7141 aacatctggt agcaaaatat cttgatggcg ttaccgtgcg taaagtgatt tacgtaccag
     7201 gtaaactcct caatctggtc gttggctaag cgcgggagga agcgtgcgat atctggcaac
     7261 attgttgtta tctctggcgg tgttaatcac cgccgggtgt ggctggcatc tgcgtgatac
     7321 cacgcaggtt ccttccacta tgaaggtcat gatcctggac tcaggcgatc cgaacgggcc
     7381 attaagccgt gcggtgcgta accagttacg tctgaatggt gtcgagttgc ttgataaaga
     7441 aaccacgcgt aaggacgttc catccctgcg tttgggtaaa gtgagcatcg cgaaagatac
     7501 cgcatcggta ttccgtaacg gtcaaacagc agagtatcag atgatcatga cggtgaatgc
     7561 gaccgtgttg atccccggcc gtgatatcta cccgattagt gccaaagtct tccgttcgtt
     7621 ctttgataac ccgcaaatgg cgttagcgaa agataacgaa caagacatga tcgtaaaaga
     7681 gatgtacgac cgtgctgccg aacagctgat tcgtaagctg ccaagcatcc gtgctgcgga
     7741 tattcgttcc gacgaagaac agacgtcgac cacaacggat actccggcaa cgcctgcacg
     7801 cgtctccacc acgctgggta actgatgatt cggttgtacc cggaacaact ccgcgcgcag
     7861 ctcaatgaag ggctgcgcgc ggcgtatctt ttacttggta acgatcctct gttattgcag
     7921 gaaagccagg acgttgttcg tcaggtagct gcggcacaag gattcgaaga acaccacact
     7981 ttttccattg atcccaacac tgactggaat gcgatctttt cgttatgcca ggctatgagt
     8041 ctgtttgcca gtcgacaaac gctattgctg ttgttaccag aaaacggacc gaatgcggcg
     8101 atcaatgaac aacttctcac actcaccgga cttctgcatg acgacctgct gttgatcgtc
     8161 cgcggtaata aattaagcaa agcgcaagaa aatgccgcct ggtttactgc tcttgcgaat
     8221 cgcagcgtgc aggtgacctg tcagacaccg gagcaggctc agcttccccg ctgggttgct
     8281 gcgcgcgcaa aacagctcaa cttagaactg gatgacgctg caaatcaggt gctctgctac
     8341 tgttatgaag gtaacctgct ggcgctggct caggcactgg agcgtttatc gctgctctgg
     8401 ccagacggca aattgacatt accgcgcgtt gaacaggcgg tgaatgatgc cgcgcatttc
     8461 accccttttc attgggttga tgctttgttg atgggaaaaa gtaagcgcgc gttgcatatt
     8521 cttcagcaac tgcgtctgga aggcagcgaa ccggttattt tgttgcgcac attacaacgt
     8581 gaactgttgt tactggttaa cctgaaacgc cagtctgccc atacgccact gcgtgcgttg
     8641 tttgataagc atcgggtatg gcagaaccgc cggggcatga tgggcgaggc gttaaatcgc
     8701 ttaagtcaga cgcagttacg tcaggccgtg caactcctga cacgaacgga actcaccctc
     8761 aaacaagatt acggtcagtc agtgtgggca gagctggaag ggttatctct tctgttgtgc
     8821 cataaacccc tggcggacgt atttatcgac ggttgatatg aaatctttac aggctctgtt
     8881 tggcggcacc tttgatccgg tgcactatgg tcatcttaaa cccgtggaaa cgctggcgaa
     8941 tttgattggt ctgacgcggg tcacaatcat ccctaataat gttcctccgc atcgtcccca
     9001 gccggaagcg aacagcgtgc agcgtaaaca catgcttgaa ctggcgattg ccgacaagcc
     9061 attatttact cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca ctgcgcaaac
     9121 actgaaagag tggcggcagg aacaagggcc ggacgtgccg ctggcgttta ttattggtca
     9181 ggattcattg ctgacctttc cgacctggta cgaatacgaa acgatactcg acaatgcaca
     9241 tttgatcgtc tgtcggcgtc caggttaccc acttgaaatg gcgcaaccgc aataccagca
     9301 atggctggaa gatcatttga cacataaccc ggaagatctt caccttcagc ctgccggtaa
     9361 aatttatctg gctgaaacgc cgtggtttaa catctcggcg accatcatcc gcgaacgttt
     9421 gcaaaacggt gaatcgtgtg aggatttatt gccggaaccg gtactgactt acattaacca
     9481 acaaggcttg tatcgctgat acctgtcgtt acggagggca tgatgcgact gtggttaatt
     9541 cgtcatggtg aaacgcaagc gaatatcgat ggtctttaca gcggtcatgc gcccaccccc
     9601 ctgaccgcgc gcggtattga gcaagcgcaa aatctgcata cactgctaga tgatgtttcc
     9661 tttgacctgg ttttatgcag tgaactggaa cgggcacagc ataccgcgcg acgggttctc
     9721 agtgaccgcc agctccccgt gcaaatcatc cctgaactca acgaaatgtt ttttggcgac
     9781 tgggagatgc gacatcatcg cgacctcatg caagaagatg ccgaaaacta tagcgcgtgg
     9841 tgcaatgact ggcagcatgc tatccccacg aacggtgaag gatttcaggc attttcgcaa
     9901 cgtgtggaaa gatttatcgc aaggcttagt gaatatcagc actatcagaa tattttaatc
     9961 gtcagccatc agggcgtact gagtctgtta attgctcgtc taattggcat gcctgccgaa
    10021 tctatgtggc attttcgcgt tgaccagggg tgttggagcg ccattgatat caaccaaaaa
    10081 ttcgcgacgc tacgcgtcct caatagccgt gccatcgggg tcgagaatgc atgacttttc
    10141 tgttttttta cgggtaagcc gcaacgacca ttgacagccc cgggcaggct gatattctcc
    10201 gcagccagac tttttccgcc agacacgact ttgtagaaat tgttttacaa aaatggcgat
    10261 gcaatctgcg gcgcggggtg ggatgatagc ccactttcga aagccgattc ggcgacaatt
    10321 gtcccgaaat cgcctctggt tcaggtatac tgacagacca tttttatcta tttgattcac
    10381 ccagggggaa aacttgcagg gtaaagcact ccaggatttt gttatcgaca aaattgatga
    10441 cctcaaaggt caggacatca tcgccttaga cgttcagggc aaatccagca tcaccgactg
    10501 catgatcatc tgtacgggta cgtccagccg tcatgttatg tccattgctg accacgttgt
    10561 gcaggagtct cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa acagcgccga
    10621 ctggattgtc gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag agagccgtcg
    10681 cctgtatgaa ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg tcgccgtggg
    10741 aacgaaaatg ccggactggg tacaaaccgg ttttaccgag tacctgcgtc gttttccgaa
    10801 agatatgccc ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga atgcggacat
    10861 caagcgcata ctcgacaaag agggtgagca gatgttggcg gccgcaggca aaaaccgcat
    10921 tgtcaccctc gatattccag gcaagccctg ggatacgccg cagttagccg ctgagctgga
    10981 acgctggaag ctggatggtc gcgacgtcag tctactgatt ggcgggcctg aagggttgtc
    11041 gcctgcctgt aaagcggcgg cggagcagag ctggtcgctg tcggcgctta ccctccccca
    11101 tccgctggtt cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca tcaccaccaa
    11161 ccatccttat caccgtgagt gataagggag ctttgagtag aaaacgcagc ggatgaaact
    11221 acagaactct tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc gggcgctggt
    11281 cgcctttttg gggattttgc tgctgaccgg cgtgcttatc gccaacctgt ataatctgca
    11341 aattgttcgc tttaccgatt accagacccg ctctaatgaa aaccgcatta agctggtgcc
    11401 tatcgcgccc agccgcggca ttatctacga ccgtaacggt atccctctgg cactcaaccg
    11461 cactatctac cagatagaaa tgatgccgga gaaagtcgat aacgtgcagc aaacgctgga
    11521 cgctttgcgc agcgtggttg atctgaccga tgacgatatt gctgcattcc gaaaagagcg
    11581 cgcacgttca caccgtttca cctctattcc cgtgaaaact aacctgaccg aagtacaagt
    11641 agctcgcttt gccgtcaatc agtaccgttt tccgggtgtc gaagttaaag gctataaacg
    11701 tcgttactat ccttacggtt cggcgttgac ccacgtcatc ggctatgtgt cgaaaatcaa
    11761 cgataaagac gtcgaacgcc tgaataatga cggcaaactg gccaactatg cggcaacgca
    11821 tgatatcggt aagctgggca ttgagcgtta ctatgaagac gtgctgcacg gtcagaccgg
    11881 ttatgaagag gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa aagaagtacc
    11941 accgcaagcc ggacacgata tttacctgac gctggatctc aaactccagc aatatattga
    12001 aacgctgctg gcgggtagcc gcgcagctgt ggtagtcacc gatccgcgta caggtggggt
    12061 gctggcactg gtttccacgc ctagttatga cccaaacctg tttgttgacg gtatctccag
    12121 caaagattat tccgccttgc tgaacgaccc gaatacaccg ctggtgaacc gcgccacaca
    12181 gggggtttat cctcctgcgt ctacagttaa accctatgtg gcggtttcgg cattaagcgc
    12241 cggggtgatc acgcgcaata cgacgctgtt tgacccaggc tggtggcaac taccagggtc
    12301 ggaaaaacgt tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg tcacaagatc
    12361 gctggaagaa tctgcggata ccttcttcta tcaggtggcc tacgatatgg ggatcgatcg
    12421 cctctccgaa tggatgggta aattcggtta tggtcattac accggtatcg acctggcgga
    12481 agaacgttcc ggcaacatgc ctacccgcga atggaaacag aaacgcttta aaaaaccgtg
    12541 gtatcagggt gacaccattc cggttggtat cggccagggt tactggacag cgaccccaat
    12601 ccagatgagt aaggcactga tgatcctgat taatgacggt atcgtgaagg ttccgcattt
    12661 gctgatgagc accgccgaag acggcaaaca ggtgccatgg gtacaaccgc atgaaccgcc
    12721 cgtcggcgat attcattccg gttactggga gctggcgaaa gacggtatgt acggtgttgc
    12781 taaccgccct aacggtacgg cgcataaata ctttgccagc gcaccgtaca aaattgcggc
    12841 gaaatctggt accgctcagg tcttcggtct gaaagcgaac gaaacctata atgcgcacaa
    12901 aattgctgag cgtttacgtg accacaaact gatgaccgcc tttgcgccat acaacaatcc
    12961 gcaagtggct gtcgccatga ttctggagaa cggcggcgcg ggtccggcgg ttggtacgct
    13021 gatgcgccag atcctcgacc acattatgct gggtgataac aataccgatc tgcctgcgga
    13081 aaatcctgcg gttgccgcag cggaggacca ttaatcatga cggataatcc gaataaaaaa
    13141 acattctggg ataaagtcca tctcgatccc acaatgctgc tgatcttact ggcattgctg
    13201 gtttacagcg ccctggttat ctggagcgcc agcggtcagg atattggcat gatggagcgt
    13261 aaaataggcc aaatcgcgat gggtctggtc atcatggtgg tgatggcgca aattcctcca
    13321 cgcgtttatg aaggctgggc cccctatctc tatatcatct gtattatttt gctggtggcg
    13381 gtagatgctt tcggtgccat ctctaaaggc gctcaacgct ggctggacct cggtattgtt
    13441 cgttttcagc cgtctgaaat tgccaaaata gccgtaccac tgatggttgc gcgctttatc
    13501 aaccgcgacg tttgcccgcc atcgttgaag aacactggca tcgcgctggt gctgatattt
    13561 atgcccacgc tgctggtggc tgcacagcct gacctgggaa catcaatcct cgttgcgctt
    13621 tccggtctgt ttgtactgtt cctctctggc cttagctggc gtctgattgg cgtcgcagta
    13681 gtgctggtgg cggcgttcat tcctattctg tggttcttcc tgatgcatga ttaccagcgc
    13741 cagcgcgtaa tgatgctcct ggacccggaa tcagacccac tcggcgcggg ctatcacatt
    13801 attcagtcta aaattgctat tggctccggc ggattacgcg gcaaaggctg gctgcatggc
    13861 actcagtcac aacttgaatt tctacccgaa cgccataccg actttatctt cgcggtactg
    13921 gcggaagagc tgggattagt gggcattctg attctgctcg ctctctacat tctgctgatc
    13981 atgcgcgggc tgtggatagc cgccagagcg caaaccacct ttggtcgcgt catggctggc
    14041 ggcttaatgc tgatattatt cgtttatgtc ttcgtaaata ttggtatggt aagcggtatt
    14101 ctgccggttg taggggttcc gctcccactg gtcagttatg gaggatcagc gctaattgtg
    14161 ctgatggctg ggttcgggat tgtaatgtca atccacaccc acaggaaaat gttgtcgaaa
    14221 agcgtgtaag aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg cggcaggaat
    14281 gctcgcggca tgtacaagcg atgatggtca gcaacagacg gtaagtgtac cgcagcctgc
    14341 ggtatgtaac ggccccatag ttgaaattag cggggcggac ccgcgtttcg aaccactgaa
    14401 cgcgacggca aatcaggatt accagcgcga cggtaaaagc tacaaaatcg tgcaggatcc
    14461 gtctcgattt agccaggcgg gactggcggc aatctatgat gccgaaccag gcagtaacct
    14521 gacggcctct ggcgaagctt tcgatccgac acagttgacg gcggcccatc caacgcttcc
    14581 gatccccagc tacgccagaa tcactaacct ggctaacggg cgaatgatcg tggtgcgcat
    14641 taatgatcgc ggcccctacg gcaacgaccg cgtaatttcg ctttctcgcg cagcagctga
    14701 ccgtcttaac acgtcaaaca acaccaaagt tcgtatcgat ccgattattg tcgcccagga
    14761 tggttcgctt tctggtcctg gtatggcttg taccacagtc gccaaacaga cttacgccct
    14821 gcctgcacct cccgatttaa gcggtggcgc gggggcaagt tcagtgtctg gcccgcaggg
    14881 tgacattctt ccggtcagta actcgacgct aaaaagcgaa gatccgaccg gcgcgccggt
    14941 aaccagtagc ggtttcctcg gcgctccaac gaccttagcg cctggtgtac tggaaagcag
    15001 cgaaccgaca cctgcaccac agcccgttgt tacagctccg tcgacaacgc ctgcaacctc
    15061 gcctgcaatg gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact ttatggtgca
    15121 agtcggggcc gttagcgatc aggctcgtgc gcaacagtac caacagcaac tgggacagaa
    15181 gttcggcgtc cccggtcgcg taactcaaaa tggcgcggtc tggcgtatcc agcttggccc
    15241 attcgccagt aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg aagcccaatt
    15301 acagtcattt attaccaccg cgcagtagcg taaagcaggc atctgatgtg tcaatttctg
    15361 taaatgacgt taacaaagtc acgcggaaag tcagatgcct gccggtagtg catttgctat
    15421 agtagggcac tttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt
    15481 tttccgctcg tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct
    15541 ctgctgcaca tgccgatgac ctgaatatca aaactatgat cccgggtgta ccgcagatcg
    15601 atgcggagtc ctacatcctg attgactata actccggcaa agtgctcgcc gaacagaacg
    15661 cagatgtccg ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc
    15721 aggcaatgaa agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat
    15781 gggccaccgg taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc
    15841 aggttccggt ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgcg
    15901 tcgccatggc cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct
    15961 acgttaacgc cctgggcctg aaaaacaccc acttccagac ggtacatggt ctggatgctg
    16021 atggtcagta cagctccgcg cgagatatgg cgctgatcgg ccaggcattg atccgtgacg
    16081 taccgaatga atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc
    16141 tgaaccgtaa cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac
    16201 acactgacaa agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga
    16261 tttctgcggt gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaactgc
    16321 taacctgggg cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg
    16381 cctctgaacc ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg
    16441 tgtacctgac cattccacgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca
    16501 gcagtgaatt gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc
    16561 ttgatggcaa aacgatcgag caacgcccgc tggttgtgtt gcaagaaatc ccggaaggta
    16621 acttcttcgg caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa
    16681 aattgaacac ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaaactc
    16741 ccgccttctg gcgggagttg ctatttaatt acgttacgcc ggagctgaca tgaaaaccaa
    16801 acttaacgaa ctgcttgaat tccctactcc ttttacttac aaagttatgg ggcaggcgtt
    16861 acctgagctg gttgatcagg tggttgaagt ggtacagcgc catgcgccag gtgactacac
    16921 cccaacggta aaaccaagca gcaaaggcaa ctaccactcg gtatctatca ctatcaacgc
    16981 cactcatatc gagcaggttg aaacactgta tgaagaactg ggcaaaatcg atattgtccg
    17041 catggttctg taactcgctt ctccgttacc cggctcctgg tcgggtaact cccctctcct
    17101 cgctgtgata tacttttccc ccacttttac tcattctcca cggagatgcc gttttgtatc
    17161 aggataaaat tcttgtccgc cagctcggtc ttcagcctta cgagccaatc tcccaggcta
    17221 tgcatgaatt caccgatacc cgcgatgata gtacccttga tgaaatctgg ctggtcgagc
    17281 actatccggt attcacccaa ggtcaggcag gaaaagcgga gcacatttta atgccgggtg
    17341 atattccggt gatccagagc gatcgcggtg ggcaggtgac ttatcacggg ccgggacaac
    17401 aggtgatgta tgtgttgctt aacctgaaac gccgtaaact cggtgtgcgt gaactggtga
    17461 ccttgcttga gcaaacagtg gtgaataccc tggctgaact gggtatagaa gcgcatcctc
    17521 gggctgacgc gcctggtgtc tatgtcgggg aaaagaaaat ttgctcactg ggtttacgaa
    17581 ttcgacgcgg ttgttcattc cacggtctgg cattaaacgt caatatggat ctttcaccat
    17641 ttttacgtat taatccttgt gggtatgccg gaatggaaat ggctaaaata tcacaatgga
    17701 aacccgaagc gacgactaat aatattgctc cacgtttact ggaaaatatt ttagcgctac
    17761 taaacaatcc ggacttcgaa tatattaccg cttaattcca tacatcaatg gcccaattta
    17821 cactgggtca ttactcataa tacctgctaa tgctttacat ctccttcgcg tttgtatatt
    17881 ttctttatca cttgcagaaa tgcggtgcca cgccttgctc cgcaactcac cccattgatt
    17941 aacctattga aatagttgat tatggtgagc aaaagcgttc cgttccaatt agacataaga
    18001 ttattgtaat tttttaaata ttatttttcc atgaataaaa ttggagtaag tgcgtggata
    18061 gtaataatca aattgaaccc tgtttaagca gaaagtcgtc agaaggtaaa ccacaaatat
    18121 ttacaacctt acgaaatatt gatcttaacc ttctgactat ttttgaagct gtatatgtac
    18181 ataaagggat cgttaatgca gcgaaagtgc ttaatctgac cccctcggca atcagtcagt
    18241 ctattcagaa actgcgcgtt atattccctg acccattgtt tattcgcaaa ggccagggtg
    18301 tcactcctac cgcatttgcg atgcatctac atgagtatat cagtcagggc cttgagtcca
    18361 ttcttggtgc gctggatatc gaaggaagct atgataagca acgaacgata actattgcga
    18421 ccactccctc ggtcggagcc ttggtccttc ctgtcatcta tcgggcgatt aaaactcact
    18481 atccgcagct tttactgcgc aacccgccca tcagcgacgc ggaaaaccaa ctcagtcagt
    18541 ttcaaaccga tctcatcatc gataacatgt tttgcaccaa tcgtacggtg caacatcatg
    18601 ttctgttcac cgacaatatg gtgttaattt gccgcgaggg aaatccacta ctctctttag
    18661 aagatgacag agagactatc gacaacgctg cgcatgtact tctgttaccg gaagagcaaa
    18721 atttcagcgg tctgcggcag agagttcaag aaatgtttcc ggaccggcaa atcaatttca
    18781 ccagctacaa cattttgaca atcgctgcac tggttgccaa cagtgacatg ttagcgatta
    18841 ttcccagccg tttttataac ctgtttagcc gctgctggcc gctggaaaaa ctgccttttc
    18901 cgtccttaaa tgaggagcaa atagatttct ctatccacta caacaaattc agcctgcgtg
    18961 atcccatcct gcacggagta atagatgtta tccgtaatgc attttgaaaa agtattgtta
    19021 cagccagatc acagcgcaga acgataaaat ggcacaatcc aacaacaatt ttacattttc
    19081 gcgaccgctt tggctgcttt caggtccgtt tcaatgatat actgccagtc gttaattcaa
    19141 aaatagttga taattacaac aatctattga attgaaacgc tttccttcgt aattcgcaac
    19201 tggaacacgc acgctatgag taaacccatt gtgatggaac gcggtgttaa ataccgcgat
    19261 gccgataaga tggcccttat cccggttaaa aacgtggcaa cagagcgcga agccctgctg
    19321 cgcaagccgg aatggatgaa aatcaagctt ccagcggact ctacacgtat ccagggcatc
    19381 aaagccgcaa tgcgcaaaaa tggcctgcat tctgtctgcg aggaagcctc ctgccctaac
    19441 ctggcggaat gcttcaacca cggcacagca acgtttatga tcctcggcgc tatttgtacc
    19501 cgccgttgtc cgttctgtga tgttgcccac ggtcgcccgg tagctcctga tgccaatgaa
    19561 ccagtgaaac tggcgcagac cattgccgat atggcgctgc gttatgtggt tatcacctcc
    19621 gttgaccgtg atgacctgcg cgatggcggt gcccagcact ttgcggattg cattactgcc
    19681 attcgggaaa aaagcccgca aatcaaaatt gaaactctgg tgccggattt ccgtggtcgt
    19741 atggatcgtg ctctggatat tctgactgca acgccaccag atgtgttcaa ccataacctg
    19801 gaaaacgtac cgcgtattta ccgtcaggta cggcctggtg cagattacaa ctggtcgctg
    19861 aagctgctgg aacgctttaa agaagcgcat ccggaaatcc cgaccaagtc tggtctgatg
    19921 gtgggactgg gtgaaaccaa tgaagaaatt attgaggtaa tgcgcgacct gcgccgtcat
    19981 ggtgtgacga tgttaacgct ggggcaatat ttgcagccaa gccgccatca cctgccggtt
    20041 caacgttacg ttagcccgga tgagttcgac gaaatgaaag ccgaagcgct ggcgatgggc
    20101 tttacccatg ctgcatgcgg tccgtttgtc cgctcttctt accacgccga tttgcaggcg
    20161 aaagggatgg aagttaagta atcctgtaat aattacttac atctgtctga taaaaaaccc
    20221 gctttttgaa gcgggttttt tgtatcgaac aagatattga gggagcgtcc tgctcgccac
    20281 gtcactcttt atgagagagc ttttcagcct gaagatcaac gtctgcgcct tttttcgccg
    20341 cagcatcgtc atcattcatc gccttcttga accctttaat ggccgctcca aggtctccgc
    20401 ccagcgtacg taacttctta gtcccaaaca gcagaacgac cagcgccgca actactagca
    20461 gtttggtaat actaatctca cccatagata ccttcttgac atataaccgg caacagaacg
    20521 cgccttaaaa accagagaat attaacggtc attcgacgcg cgcacacaat agcaatctgt
    20581 aacaaggtga atcaagcgcc gagtaaaaaa acatcataat aattgcggcg gcgcaaagcg
    20641 acggttgttt aagacgggca gttgcgcgcg cacctgacgc acacgttcgg gcgtcacttc
    20701 cgccataatg agtgcaggca tttctgacgc tgccgcaatg gtgacgccaa agggatctat
    20761 aatccggctt tgaccgatat ttttgttccc gcactccccc gccgccacca tataacaggt
    20821 ggtatccagc gcacgagcgg caagcaacgt tgaccaatga tgctctttga gcggcccgcg
    20881 aacccaggcg gcaggaagta ccaggatttc agctccctgt aatgcctgtg ccagcgccag
    20941 ctctggaaag cgtaagtcat aacaggtcat cagaccgacc ttcatcccct ccacctcgag
    21001 taacggagcg atttcattac cagcatcaac acggcgtgat tcctgaatgg caaatgcatc
    21061 atagagatgc aatttggcat aacgggcgac gatgtttcct gcctgtagcg ccaccagcat
    21121 attccatgcg cgccccggcg ttgaaggaac atgaatcgtc agaattgtcg tcatcatgtt
    21181 acgtttactt tctcgccgta aacgtccgag gaattcgcct tccagcagct gtgctgattt
    21241 aaccgataga tctgcatcat gatcatcgcg cgccagcaat gcttccggca ggacgaacag
    21301 cgatacgtcg ttttccgccg cctgcgccat caacgaggca caaatctcag cgttcttttc
    21361 ccacacagat gtaacagcaa actgtcctgc ggcaaccagc attgccacct cctgtcagag
    21421 tcgtaaaatt cgccaacgac gattattcgg cgttacactt atcactcata caaatcaaat
    21481 agcaggattt tgcagtgtta caacttcttt tagcagtttt tattggcggt ggtacgggaa
    21541 gcgtggcgag atggctgtta agtatgcgat ttaacccgct gcatcaggcc attccgttgg
    21601 ggacgctggc agcaaacctg attggggcat tcatcatagg aatgggattc gcctggttca
    21661 gcaggatgac gaacattgat ccagtgtgga aagtattaat caccaccgga ttttgtggcg
    21721 gtctaacaac cttctcaaca ttttcggcag aagtggtgtt tttgttacaa gagggccgct
    21781 ttggctgggc gttactgaac gttttcgtca accttctggg gtcttttgcc atgacagcac
    21841 tggctttctg gctgttttcg gcctcaaccg cacactaaag accaaaaaaa aacccgctga
    21901 ttaagcgggt tttgaattct tgctgacgta tcttacagag cgattacgtt tgcagcagaa
    21961 gggcctttgg caccgttagt gatttcgaac tctacgcgct gaccttcagc aagagtttta
    22021 aaaccattag tctggattgc agagaagtgt acgaacacgt ctttgctgcc gtcttccgga
    22081 gtaatgaaac cgaatccttt ggactcatta aaccacttaa cgttaccttt aatcttagac
    22141 atcaaaatta cctttacatg aaaaatagac acaaatgctg tgtcggttac cagtacacca
    22201 attgtggtac gctttgtcca gtgcaacttt actaaaaagt gataaaagtc gcgttatttt
    22261 tgatgttact tattttagct attgatttta aagaagttac taaaacttca tttgtctcaa
    22321 aactgaaagc gcatccaggc aaagtacaca ttgccattgt tgtaggtacc cggaatgtag
    22381 gtcatctgaa aagtcactgg gccataaccc acggaggcca atggcagtag aaccgggaga
    22441 gggatgtaat tccagttatc gcgtgccgtt acgccagcgg tgaatcccag acctaaatga
    22501 aaattttcat ccgccagcgg tcgccaggta ctttcccatc cgtatccggc aatcggttcc
    22561 catttgttcc acgagtcctt aaatgccatg gcatacaggc catgccagtt tcctttttca
    22621 tcccaacgcg acaggccaaa accgccaccc cacggtcgct cgttatagcg atcggttttt
    22681 tctttgtcgt aagcgaaacg tgcatgccag gtgatggcag gaatatataa atcataatgt
    22741 tcaggctgtt gccaggtttg tgcaatattt tctctaaacg ttgtcatcca ctcatctgcg
    22801 ttagcaaaaa ctttaccaac gctgattaac tgaataaaaa caaaggaaaa gatagcgaca
    22861 tatttactca cgttcatttg tgaccataaa acatttatca aaaatctact actagcatag
    22921 caaagctaca attaacataa ccttaataga cccaacataa agaataatct gaatagctgc
    22981 tctatttatt tccgcttgtt caagttaaat atttccgcgc atcgattaaa gatgatgcgt
    23041 cgttttctgc gatgggaata gtcaaaaaag aaaaa
//
LOCUS       DAZGXZ010000079        22834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000079 DAZGXZ010000000
VERSION     DAZGXZ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.079"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..899)
                     /locus_tag="ACHKJF_003174"
     CDS             complement(<1..899)
                     /locus_tag="ACHKJF_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0950487.1"
                     /translation="MRVLLRPVLVPELGMVLLKPGRESMSAFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLAVWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPVEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMKRPKHRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGT"
     gene            complement(901..1179)
                     /locus_tag="ACHKJF_003175"
     CDS             complement(901..1179)
                     /locus_tag="ACHKJF_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950488.1"
                     /translation="MAQDTKLHNSDNSAAPASRHGRRSHAFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1315..1572
                     /locus_tag="ACHKJF_003176"
     CDS             1315..1572
                     /locus_tag="ACHKJF_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0950489.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            1578..1877
                     /locus_tag="ACHKJF_003177"
     CDS             1578..1877
                     /locus_tag="ACHKJF_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0950490.1"
                     /translation="MTYEVIITPEAEQQIINLHRYITEKAGNVIADNYANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(2080..2595)
                     /locus_tag="ACHKJF_003178"
     CDS             complement(2080..2595)
                     /locus_tag="ACHKJF_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950491.1"
                     /translation="MSEINYQEGHETAGQAKPVAWRYRYVKKDVTDFQGKPWAGDWKY
                     VPTKEDCNDRPNYEIQALFIGPPVPVTSEGLVKAVRFYEQVKRENPPVETGAWKDAVD
                     WVLREACCAAILGKADNPPASGNQVSELAMWVKRLVSQLKKAKPDCKLPDKAMDYLKR
                     NGLINEEDILR"
     gene            complement(2592..2768)
                     /locus_tag="ACHKJF_003179"
     CDS             complement(2592..2768)
                     /locus_tag="ACHKJF_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950492.1"
                     /translation="MADFGSTKYNVSFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            complement(2761..2943)
                     /locus_tag="ACHKJF_003180"
     CDS             complement(2761..2943)
                     /locus_tag="ACHKJF_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950493.1"
                     /translation="MRKFKIIIETGIAGGDFEDVFEVDDDATPDEIHDEAKEIFFNYC
                     DYSYHEIKDEEEEQNG"
     gene            complement(3091..3396)
                     /locus_tag="ACHKJF_003181"
     CDS             complement(3091..3396)
                     /locus_tag="ACHKJF_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0950494.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQEATSERN"
     gene            complement(3393..3674)
                     /locus_tag="ACHKJF_003182"
     CDS             complement(3393..3674)
                     /locus_tag="ACHKJF_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0950495.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKAREPGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGVPRREIAEKFGMKLRAVNNYVYFDRRIQA"
     gene            complement(3707..4468)
                     /locus_tag="ACHKJF_003183"
     CDS             complement(3707..4468)
                     /locus_tag="ACHKJF_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0950496.1"
                     /translation="METVFDALQAMGKATSIELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGKGESGVTEEQPAQSEVPDVLTGEVEQKVTADMMIEFICQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCIPGADLPAEPEAASVAETDGKA
                     FPQPAGVALPVREAETQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRA
                     KGQVQKWERVCAALRELNKCRDILRDITATREQQR"
     gene            complement(4502..4963)
                     /locus_tag="ACHKJF_003184"
     CDS             complement(4502..4963)
                     /locus_tag="ACHKJF_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0950497.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESEANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSIPPIRRQLASPKRPQGPTPIELLKQEYERRKAAGFV"
     gene            complement(4956..5993)
                     /locus_tag="ACHKJF_003185"
     CDS             complement(4956..5993)
                     /locus_tag="ACHKJF_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0950498.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTTCHGVSLRGHIDVTTEDLASALDVTEDVIDSILHAMQGRVLDGDLISGWEKRQV
                     LKEDNGNVSQTAKSPAERKRAQREREKLRKYDADCHDESRRVTHLSRQVTTDKDTDTE
                     LNPTHNARMRESAPTGESNGASLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAA
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAASGGTASRAVQKIRAAREQWERENGFISNGNGLEAVGTYGGGVFEPMDPEERGCAV
                     ETLDCSDWRDD"
     gene            complement(6062..6487)
                     /locus_tag="ACHKJF_003186"
     CDS             complement(6062..6487)
                     /locus_tag="ACHKJF_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0950499.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTSDAVEKIQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRERLVKDVDDFVAVAIAWGTLTNSGGQPGNAVVVH"
     gene            complement(6471..6746)
                     /locus_tag="ACHKJF_003187"
     CDS             complement(6471..6746)
                     /locus_tag="ACHKJF_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0950500.1"
                     /translation="MNLGEYLHHSGITQKHFAEIVGVTQGMVSHVITGRAKLTGEKIL
                     RWCEATGWIVTPHEIDSSTYPNPTDGLPVEYQANTQPEAGVIHENQA"
     gene            6854..7354
                     /locus_tag="ACHKJF_003188"
     CDS             6854..7354
                     /locus_tag="ACHKJF_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0950501.1"
                     /translation="MKRKSLSEIDLQAAQRLKEIWTAKKNQLGLTQERAAEILGFSTQ
                     GAVSHYLNGQTPLNLEAVIKFAGLLQVPPESIRPDMAELLQIVRMYPQESGEDNVVTI
                     SADMEQSENELPFNIDPMERDLLQTFRAFPKEDKEKMLKEMKEKKESIEEIVARWLAA
                     QKGRRA"
     gene            7372..7563
                     /locus_tag="ACHKJF_003189"
     CDS             7372..7563
                     /locus_tag="ACHKJF_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP0950502.1"
                     /translation="MSTALSPIISEFETVEQENSYNEWLRAKVAASLSDPRPAIPHDE
                     VMAEMENLIAQLAATNRSE"
     gene            7563..7853
                     /locus_tag="ACHKJF_003190"
     CDS             7563..7853
                     /locus_tag="ACHKJF_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0950503.1"
                     /translation="MLPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLT
                     EHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIEIVNVVHSRRQYPNKTC"
     gene            8121..8273
                     /locus_tag="ACHKJF_003191"
     CDS             8121..8273
                     /locus_tag="ACHKJF_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0950504.1"
                     /translation="MDTLNLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRW
                     LARNTN"
     gene            8769..9623
                     /locus_tag="ACHKJF_003192"
     CDS             8769..9623
                     /locus_tag="ACHKJF_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0950505.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTSRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAPSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            9634..9822
                     /gene="dicB"
                     /locus_tag="ACHKJF_003193"
     CDS             9634..9822
                     /gene="dicB"
                     /locus_tag="ACHKJF_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0950506.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIFGEHQ"
     gene            9819..10007
                     /locus_tag="ACHKJF_003194"
     CDS             9819..10007
                     /locus_tag="ACHKJF_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0950507.1"
                     /translation="MTSAFALVMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPLKKVSLYLNNETPAG"
     gene            10100..12544
                     /locus_tag="ACHKJF_003195"
     CDS             10100..12544
                     /locus_tag="ACHKJF_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0950508.1"
                     /translation="MSTDKEEFSLYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PLVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSEKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLDADYTDRGLLTKEWMKGNRVLRISRTPSGANAGGGILTDRGEGFVHDDASVE
                     RDVAAGVLARSMDIDIYNPHPAHAKRIEEIVSENKPPFSVFRDKFIAMPGHLDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LYEARKNIPPKNPVDADKLLAASHGEFVEGISDPNDPKWVKGIETRDSVNHNQQETEQ
                     KGHKAEQHSPNAQQNEPETKQPEPVAQQEPEKVCTACGQTGGGNCPDCGAVMGDATYQ
                     ETFNEESQDEAREKDPEEMESAGLPNKECTEGDQHANGNNETGETANPLIKVNGHREI
                     TSTSRLWHHLMIDLETMGKNPDAPIASIGAVFFDPQTGEQGPEFSKIIDMGTCGGTVD
                     ISTIEWWLQRSGEARAAILADRIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVI
                     LRRSYERQEIPCPWRYTNDRDVRTMVALGLVMDFDARNVTTFEGERHNALHDARYQAK
                     YVSAIWQKLFPNQADF"
     gene            12603..12806
                     /locus_tag="ACHKJF_003196"
     CDS             12603..12806
                     /locus_tag="ACHKJF_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP0950509.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRNWLIKNGLPFYTNRSGKPIV
                     SRELFTCRNTLPPREAEPDFGAI"
     gene            12806..13831
                     /locus_tag="ACHKJF_003197"
     CDS             12806..13831
                     /locus_tag="ACHKJF_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0950510.1"
                     /translation="MGRRRKNPEHEKLPPKVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNHHDHAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVL
                     ADSVKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPVPVFIAMEIAYLCAARISDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWTPRLQSVIEKAKQLPKSAYVISNQYGNRYMYKGFNEMWVEARNLAGQIS
                     GIATDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKISPTLDVPLPQN
                     IPTKYSK"
     gene            complement(13884..13973)
                     /locus_tag="ACHKJF_003198"
     tRNA            complement(13884..13973)
                     /locus_tag="ACHKJF_003198"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13937..13939),aa:Ser,seq:cga)
     gene            14067..14864
                     /gene="mtfA"
                     /locus_tag="ACHKJF_003199"
     CDS             14067..14864
                     /gene="mtfA"
                     /locus_tag="ACHKJF_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP0950511.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            14965..15040
                     /locus_tag="ACHKJF_003200"
     tRNA            14965..15040
                     /locus_tag="ACHKJF_003200"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14998..15000,aa:Asn,seq:gtt)
     gene            15202..16458
                     /locus_tag="ACHKJF_003201"
     CDS             15202..16458
                     /locus_tag="ACHKJF_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0950512.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLLVKPGGSRHWYLKYRI
                     NGKESRIALGAYPAVSLSDARQQREGVRKMLALNINPVQQRAAERGSRTPDKVFKNVA
                     LAWHKSNRKWSQNTADRLLASMNNHIFPVIGNLPVSELKPRHFIDLLKRIEEKGLLEV
                     ASRTRQHLSNIMRHAVHQGLIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIEA
                     YHQGRELTRLAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLDICRKAYVSPYMMVQENR"
     gene            16643..18616
                     /locus_tag="ACHKJF_003202"
     CDS             16643..18616
                     /locus_tag="ACHKJF_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017382560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoX family phosphatase"
                     /protein_id="HFP0950513.1"
                     /translation="MSSPLKSVFKKEHSDEISNHSVNPVFSEVVSAFMSRRRFLQMGM
                     VAGAAVSFPYLVKPENAFAAKANPSALSKAVSLGFTSIPVSTADTVTVPEGYIARPFY
                     RWGDAVGIKGNLPEFKFDASNTTDEQAAQAGMHHDGMAWFSLPQGKENPAHGLLALNH
                     EYIDNGMLFKDGTANWDLDKARKGQNAMGISVIEVKKDNVGWQVVRPSSFARRITVNT
                     PMQLSGPARHQALMKTAADPQGEVVLGTMQNCANGKTPWGTYLTCEENWSDIFVKKAP
                     RNVLEKRYGISDSDESYRWNEVDERFSVDKTPNEPNRFGWVVEIDPYDPTSTPRKHTA
                     LGRFKHEGAAVTLAGDNRVVVYMGDDQKFEYIYKFISENKYDPGDRKANMQLLESGTL
                     YVARFNDDGSGDWLPLIFGENGLDQSKGFDNQGDLLIKTRLAADTVGATKMDRPEWIA
                     VDTHAKGSVYCTLTNNSDRGKEGKAPVDAANPRANNQFGHIMHWREERADPASAKFTW
                     NILVLAGRTDSDDPKAKGSMQGAEFGSPDGLSFDHRGVLWIQTDVSSSTINKKAYEGM
                     GNNQMIATLPGTNEYRRFLTGPRGCEITGIAFTPDNRTLFINIQHPGEGGDDITDPSN
                     PRAISNWPDSRSDGRPRSSTVVITKSNGGIIGT"
     gene            18895..19632
                     /gene="aztA"
                     /locus_tag="ACHKJF_003203"
     CDS             18895..19632
                     /gene="aztA"
                     /locus_tag="ACHKJF_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein AztA"
                     /protein_id="HFP0950514.1"
                     /translation="MHDYSLRFANLALGYEGLPAIQNITGTIQKGSLTAIIGPNGSGK
                     STLLKGIAGILAPLSGSCTVEPKARIAYLPQISELDRTFPATVSDLVSLGLWPERGLF
                     RHHRIEDRKRLTDALGSVGLAGFEKRQLSALSGGQLQRALFARVILQQANIILLDEPF
                     NAIDATTIDDLLVLINRWHAQKRTVLAVMHDIGLVRNHFPQAILLNGKLVAWDETEQV
                     LRHTSLLPAQNMGTARALPGQHEATHS"
     gene            19629..20486
                     /gene="aztB"
                     /locus_tag="ACHKJF_003204"
     CDS             19629..20486
                     /gene="aztB"
                     /locus_tag="ACHKJF_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HFP0950515.1"
                     /translation="MNWLHEWVLAPFVGFGFMRRALYGSFLLCLSACPVGVFLTLRRM
                     SLVGDAMSHAILPGAAFGFLLYGLDILPMTIGGLVAGLIVAIFAGAVSRLTVQKEDAS
                     MAAFYLISLAVGVLIVSLRGSSVDLMHVLFGTVLALNHEALLLIFSVALLTLLLVSIF
                     WRALIAECLDPLFLRSVSRLGSPVHFIFLALVVLNLVAGFQALGTLLSVGVMVLPAVT
                     ARFWTRRVATMCIASVLIGMLACISGLLFSFHFSLPSGPSIILSVGIMYLVSAAAMMV
                     KNIHLKKLR"
     gene            20518..21468
                     /gene="aztC"
                     /locus_tag="ACHKJF_003205"
     CDS             20518..21468
                     /gene="aztC"
                     /locus_tag="ACHKJF_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AztC"
                     /protein_id="HFP0950516.1"
                     /translation="MRKPGFISLCILTTSMLFLLFTFPAKAKINVIASFSVIGDMAKN
                     IGRDRIELRTIVGPNGDAHVYEPSPADAIAMSKADVILVNGLQLEGFISRLIEASETS
                     APVIETTKGANILQDPAGGHYHFYDGKAVFHAAPFDPHAWQSVGNSRIYVKNIAEAFC
                     AQDKSNCDFYQANARAYDAKLADLENKIIKTVATIPQEKRTVVVGHNAFKYFEQAYGI
                     HFLSPQGISTESEASAADVAGILKKIKENNASAVFSENISNPRLVEQIAREAGLNVAG
                     VLYTDALSEPGGPASTYLEMMEHNVTAIATAASNSRNTQP"
     gene            21507..22757
                     /gene="aztD"
                     /locus_tag="ACHKJF_003206"
     CDS             21507..22757
                     /gene="aztD"
                     /locus_tag="ACHKJF_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallochaperone AztD"
                     /protein_id="HFP0950517.1"
                     /translation="MKKRLLSTSISTLLLGLSVMPAFADEDVTAWRLFIADHDKPVVN
                     VIDALDGDKLATFNVKGPANLSRSESGATIFAIQGSAGVVSTIASGIAFHDHGDHADI
                     DIDAPKLLPLELTGKKPGHFVERQGKIAQWFDGEDSAQILGESAVLKGQKNITKVNVV
                     APHHGVAVPYDNYAVVSIPNPDDASKRPVGARVVDLQGKKVGDDALCPGLHGSAGSGD
                     TFALSCETGLLLITQKNAAPVIRHLPYAKTLPEGSTSTLIGGKGMQYFIGNYGPDRII
                     LVDPTESDSFRLIQLPTRRVHFVVDPVRAKFAYVFTEDGKLNQIDVLKGEISQSVRVT
                     DPYSMDGHWNDPRPRIAVADNKIYVTDPLKSKIIVLDATSFKKTSEISVEGQPFNIVA
                     VGGSGKVHGEHHDHEAHHHDDHAH"
BASE COUNT         5863 a         5709 c         5370 g         5892 t
ORIGIN      
        1 tttgttccca ttcccccctg accatgacca atcaggtgat gcggatcgtc agctggctta
       61 ccacaacacg cacacggctg tgtctttacc cagcgcgtgt atttctcatt aacccaacgg
      121 cgatgtttag gccgcttcat aaacgattca ggagactccg gatcaacggc gatactgaca
      181 accgtttttt tctgtggtgg attttgttgc tggtggacgt gaagtggcag cgcaatattt
      241 tttgtgcgct gcttcagtat gctgatggct gtctgttctc ccggtacgat gtcactctca
      301 cggtatacgg agcggatttt ttccaccggt aatcccagcg aacgacgcgc tactgcctca
      361 ggtagtgcat ccaccacctg attgcaggcc gcccaccagg ataattcggc cagcgataac
      421 tccctctcct gcgtaccgct tattgcgtga cgtataacat caatcatcca gactgccaga
      481 ttctgctgag caagttgatc gagtgattct gatgtctggt cgcgcagctg gttgtcacag
      541 tgccagcaca acaccatcgc gccggtaccg taacggtgaa tgacggtttc gctgtgatga
      601 taatcgccgt gtggccactg gcaggatttc acgtgacgta ataaccagtc agacagtgca
      661 cctgcaccac ctgctgcacg aataacccgc tcatcgctga aaaatggcag taatgtttta
      721 tcctctgcca gcggctggcg aacggcagga acgactccgg acggcagacc gcgcatgttt
      781 ttcggttccg gctccaccaa tattctgccg ttatggaatg ctgacattga ttcacggccc
      841 ggcttaagga gcaccatccc gagttccggt accagaacag gtcgaagtaa tacccgcacg
      901 ttacctccag atacgctgct ggaatgtgcg ggacggacgc ggtgggcgtt cggaataagg
      961 gagcctgacg gagattatcc agtgacgacg atcgaagctg agatctttct gaaactcgta
     1021 accacgtctg cggtagcact ggatcagcca ttcggcctgt tcttcagtgc atgggtcatg
     1081 ctggaaccag tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
     1141 ggagttatcc gaattgtgta atttggtatc ttgcgccatc ggttgtctct gctggcgcag
     1201 caggtgccag ttgttcaggc tggcgtgcgg caatattgtc tctgatttct gttgtcgtca
     1261 acaggcagcg tgctatcatc gaatagtgtt ctatcctact ccgtgaggtt taccatgcgt
     1321 acaacccaac aattcagcat tacattaact aacgagatgg ctgacatggt gcgcgcccgt
     1381 gtggcttccg gtgcctatgc ttcagaaagc gaggtcattc gtgaagggct tcgcgcactg
     1441 aatgagcgcg ataaagcaat cgaagcgtgg ttaacgcatt cagccgcccc ctctcttgat
     1501 tctatccgcg aaaacccaaa caacggacgc tccatttcac aggttcgcgc cgcgattcga
     1561 tccgggaagt aatctgcatg acatatgaag tcatcattac tcctgaggcc gaacaacaaa
     1621 taatcaacct gcacagatat ataacggaga aagcagggaa cgtcattgct gacaattatg
     1681 ccaatgcgct tcttgattat cttgatgggt tttctacatt cccgcatcgg ggcaataaac
     1741 gcgatgatat tcgccagggg atgcgggtaa ctcatttccg ccacagaacg attattgctt
     1801 ttgccgttga tggcagaaaa gtctttattg tcggtatcta tcatggtggg caaagttatg
     1861 aaaccgattt cttataaact tttacccata tcattccggt gttagaataa accgtctgcc
     1921 cctctcttac tgcggattcg taggctatat aaatcaaaga tcccggctca tgtttgtggc
     1981 gggatctttt ttcggcaatt tatccccagc ggcaaatcga atacaccacc agcgccaccg
     2041 ccattgcaat tcctaccgtt atgaacgctt caggccaggt catcgtaaaa tatcctcctc
     2101 gtttatcagt ccgtttcgct tcaggtagtc catcgcctta tccggtaatt tgcagtccgg
     2161 cttcgctttt ttcagttggc tgaccagccg tttaacccac attgctaatt cgctaacctg
     2221 attgccggat gctggtggat tgtcggcttt acccagaatg gcagcacagc aggcctctct
     2281 gagcacccag tcaacagcat ccttccatgc tcctgtttcg actggcggat tctcacgctt
     2341 tacctgttca taaaagcgca cagctttaac cagtccttct gatgtcaccg ggactggcgg
     2401 gccgatgaat aaggcctgaa tttcatagtt cggcctgtcg ttacaatcct cttttgtcgg
     2461 tacatatttc cagtcaccag cccacggctt cccctgaaag tctgtaacgt cttttttcac
     2521 gtagcgatat cgccatgcaa ctggttttgc ctgccctgcc gtttcatgcc cttcctgata
     2581 attaatctcg ctcattcatc gccccactca tcacaatatg cttcgaccgg agtttttcct
     2641 gcttcataat catcacgcca tgcttcagca tcagcagcac tgccaccacg taactctgca
     2701 tagtccatta acagttcatg ccattcttca aaactgacgt tgtatttagt tgaaccaaaa
     2761 tcagccattt tgttcttcct cttcgtcttt tatttcgtga tatgagtaat cgcagtagtt
     2821 aaagaaaatt tcttttgctt cgtcatgaat ttcatcaggt gttgcgtcat cgtccacttc
     2881 gaatacatcc tcaaaatctc caccggctat tcccgtttca ataattattt tgaactttcg
     2941 catttcacta ccgccctgcc gggcggtctc ctgatgttct gagggtgcag aaatccctcc
     3001 ggttaaggat taaattttat ttacagtgct gattttaatt attcagttct ggattatgct
     3061 ttctctttca cctgccgtag ttcctggtaa ttaatttcgc tcactggttg cctcctggaa
     3121 aataaccgca tgtcccagtt tctccgccag tgccagttct gccttagcgc ctgccgaccg
     3181 ctgccagcct ttcagcatgt aaattgcatc cacacaacga atcattgcca tgcaaatatc
     3241 catgtaatgt ggctgagtca gcccgtccgg aagtactgcc gggtttaaaa cggtatgccc
     3301 ttcccgtttc agcgcttctt ccgccctgtg aaacgcctcg cggttgaaat tttcatatcc
     3361 cgtcatcgga ccggcgatat aaattctcac cctcacgcct gaatcctcct gtcgaaataa
     3421 acgtagttat tcactgcacg caacttcatt ccgaactttt cggcaatttc ccgtcggggt
     3481 acaccgcgct gatgcagttg ccgcgccagc tcaatatcac gctgtgaata ttttgccgac
     3541 gggtgaaaat caccacgtaa catcatactg atgcccggct cccgtgcctt cgttctcact
     3601 gccgcttcag ttcgtccgat aagtgcgcca atgcttttca ccttcatagt tcccgcacac
     3661 tggcggagta tcaggatttc agcccggcac cacgtcttcc agccactcac cgctgctgtt
     3721 ctctggtggc ggtaatatcc cggagaatat cccggcactt gttcagctcc cgtagcgcgg
     3781 cgcagactcg ctcccacttc tgaacctgac cttttgcccg gcgcagctcg cggttagcca
     3841 catgcagcga tggtaaaatc agaccatccg gatgctttct ggtgaacgac ggctgtgact
     3901 gcactgtgac cgccacactt tccgttttta tttcttcctg tgtttccgct tcccgaactg
     3961 gtaacgcaac acctgctggc tgaggaaagg ctttaccatc ggtttccgct acggatgcag
     4021 cttccggctc tgccggtaaa tcagcgcccg gtatgcagta acgaaattta ccgttctgat
     4081 ttacgcgtgc caggcgcccc gttgctgtta cgaccgccaa cgtggaagca accttgcgaa
     4141 tgctaacacc gaacttatcc gccagttcct cacacgtttt agccccatcc tgacagataa
     4201 actcaatcat catgtccgcg gtaacttttt gttcgacctc cccggtcagc acatccggta
     4261 cttcagattg tgctggctgc tcttcggtta ccccggattc acctttacca gccagaaacc
     4321 aggtgtgacc cgttttatca acgacgccat ttcttttcag ttcccacaac tcgttcagca
     4381 cttcttcacg actgatatca agtcgcgcag caagttctat ggatgtggct tttcccattg
     4441 cttgcagtgc gtcaaaaacg gtttccatta aaacgtcctc cgacaaaatc atttctcaaa
     4501 ttcaaacaaa accagccgcc ttccggcgct cgtattcctg tttcagtaat tcaattggcg
     4561 ttggcccttg cgggcgcttc ggcgatgcga gttgcctccg gatcggcgga atactcatcc
     4621 cgttaccaac atgctttgcc catttcgtca gttgccgttc cgcaagtcgt tttaactcac
     4681 cctgcgtcat ctggcgctca atccctctgg tacgcatttc gaggcagatg tggtacagca
     4741 caggctgtgg ccacgggtat ttatcactcc cgtcgtatcg ccaggattca ttgcgccagc
     4801 gccggtactc ttccatcacg gcatccaccg taagaccaaa tggatttgcc tcactctccg
     4861 aaatcagcgc aacaaactca gccaggtccg ggggccacgt ttcacccgcc cggcagcggt
     4921 ccatgcactg acggcagacc tgccggattt gctgttcagt catcgcgcca atctgagcaa
     4981 tccagagttt cgacggcgca gccccgttct tctgggtcca tcggttcgaa tacacctccc
     5041 ccataagttc ccacagcttc caggccgttt ccgttgctga taaatccgtt ttcacgttcc
     5101 cactgttctc gtgctgcccg gattttctga actgcccgtg atgccgtgcc acctgatgct
     5161 gcatggctta cccccttgct gactggtttt acctgtgccc tgacgtgctg cacgtggcgg
     5221 gcaaatttct gctcccactg aacctgcgtg aaaaccttcc cctccgccat ccagtaatcc
     5281 cggaatgcgg caagctcagc aggtgtaaat tccggctcag gcagagccat accccacact
     5341 gctgcccgtt gtcgaaaatc cggcgacggc tgccagacag tggtcatcga aaatttcccg
     5401 atcggttcgc tcaggccgtc caggtattca ggttcggctg tctgcaacga cgcaccattc
     5461 gactcaccgg ttggagcact ctcgcgcatg cgcgcgttat gtgtggggtt taattctgta
     5521 tctgtatctt tatctgtcgt gacttgtcgt gacagatgcg tgacacgtcg tgactcatcg
     5581 tgacaatcag catcatattt ccgcagcttt tcgcgctccc gctgcgctct cttgcgctct
     5641 gccggggatt ttgccgtttg cgaaacgtta ccattgtcct ctttcagcac ctgacgtttt
     5701 tcccatccgg aaataaggtc accatccaga acccgcccct gcattgcatg caaaattgaa
     5761 tcaattacgt cttccgtcac atcaagcgca cttgctaaat cttccgtcgt gacatcaatg
     5821 tgaccacgta gtgacacgcc gtgacatgtc gtgacatttc gtgacgcgct caccagaaga
     5881 tggatataca ctgccatcac tgttgcgatt ggctgtcctg ataccctggc aatcgttcgc
     5941 cacttggggt catttggcat gtcatgccac aatctgagcc aggcattagc catactcacc
     6001 tcttctgata ccgaacttta cccacgaact tccggaagaa atccggtata aatattgttg
     6061 gtcaatgcac aacaactgca ttaccaggct gaccaccact gttagtcagg gtgccccagg
     6121 cgatcgctac agcgacaaaa tcatccacat ctttcaccag ccgctcccgt cgttcgacga
     6181 tctcccggta atattcagaa ctgtgactgc gcatacgggc caccagcaaa ggcggcatcg
     6241 ccttttcgat cgccggtaac agcgcctgaa ttttttcaac agcatcagac gtgtccttct
     6301 ccacccagcg gaaaattttc tgggtattac gggccagggc ttccggatgg ctgtcgtcgt
     6361 acagttccgg gaacgtcatc cccagttcga aataagtccg ggctatttca gctgctggaa
     6421 ctttctcacc gtctggatac gcccaggcat tcattgccat gcggatgtgt tcatgcttga
     6481 ttttcatgaa tcacccccgc ctctggttgt gtgttagcct gatactcgac aggtaagccg
     6541 tcggttgggt tgggataagt actgctatca atctcgtgcg gagttactat ccagcctgtt
     6601 gcttcgcacc agcgtaaaat tttttccccc gtaagtttcg cccgtccggt aatgacatgg
     6661 cttaccatcc cttgggttac cccaacaatt tcagcaaaat gcttctgagt tataccggaa
     6721 tgatgcaaat attctccaag attcattgtt cacctcatgt gatgtcatca cgatcattaa
     6781 tagcattgtt atttttaaaa gtaaatagca tcactatttc aaagagatta ataatcttat
     6841 tagaattgaa ggtatgaaaa gaaaatccct gtcagagatc gacctgcaag ccgcccagag
     6901 actgaaagaa atctggacgg cgaaaaaaaa tcaactaggg ttaacccaag agcgtgcggc
     6961 agaaattctg ggattttcga cacagggagc tgtaagccat tatctaaatg gtcagacacc
     7021 tttaaatctt gaggctgtta tcaagttcgc agggttgctg caagttcctc ccgagtcaat
     7081 cagaccagat atggccgagt tgttacaaat tgtaaggatg tatccccaag aatctgggga
     7141 ggacaatgtt gtcactatat ctgcagatat ggaacaatcg gaaaacgaac ttccgtttaa
     7201 tatagacccc atggagcggg atttgctcca gacgttcagg gctttcccca aagaagataa
     7261 agagaaaatg cttaaggaaa tgaaggagaa aaaagaatca attgaagaaa tcgttgcgcg
     7321 atggctagcc gcgcaaaagg gtcgtcgcgc ctaatctgag gaggtcaaaa catgagtaca
     7381 gccctttccc cgataatttc tgaatttgaa acagtcgaac aagaaaacag ctataacgaa
     7441 tggttgcgag ccaaagtggc agcaagcctc tcagatcccc gtcctgcaat tccacatgac
     7501 gaagtaatgg ctgaaatgga aaaccttatt gctcaattag ctgcaacgaa caggagtgag
     7561 taatgctgcc cattttatgg ctaccttctg cacgtgatga tttacgtcag atcgtagcct
     7621 atattgctaa ggaaaaccct cccgctgcac gtagactaaa aatacgcatt gaaacatcag
     7681 ttttgtcact tactgaacac ccttatctgt acccaccgag cgaaagagtt ccaagtcttc
     7741 gtgagatagt gactcatcct aactacataa tactttaccg agtaacagca tctaacatcg
     7801 agatcgtaaa tgtagttcac tcacgaagac agtatccaaa caaaacctgt taatccttcc
     7861 tgtcaacaac caccttcggg tggttttttt cttgccacaa taatagcact gctatttaca
     7921 taattaaata gtagtggtat tgttcattca tcaacccccc cgccccacag aacgccaggc
     7981 aatacttcga gttacccggc agtggtcagg ggttaagtag ccagcccgag gcgtatgaac
     8041 atgacggcgg gaatactttg tataacagcg cagcaggttt ttagttccgc gacccggcgt
     8101 taagggtaaa tgaggtcaac atggatacgc tcaatcttgg caacaacgaa tctctggtat
     8161 gcggtgtgtt ccctaaccag gacggcacgt ttaccgcgat gacgtatacc aaaagtaaaa
     8221 agtttaaaac cgaagctggc gcgcgtcgct ggttagccag aaacactaac tgattagcac
     8281 cagtaaaaac aggttgccac tggttaattt accctgaaaa gtcagggtat aacacgaaag
     8341 cgcacggcaa agctctttcc cttagaaggc ttgtcgttag atttcttcga acgtgcgctt
     8401 ccggttgtgg caatccgcga aatggcgcgg cggtaagtat ggctggggtt tcctccattg
     8461 ctccagaaaa tgcaccgggt tgtcaggttg accatacgat taagtgacag ccccgccaca
     8521 atacccatgt gtagtctttg gtggcatcag ttctactccg tgactgctct gccacccttt
     8581 ttaaagtgaa ttttgtgatg cggtgaatgc ggctaagcgc acgcggaaca gttaaaacaa
     8641 gctgtctttt actggcgtaa cagacatcaa ctaacaatcc ggcgttaatt gttaactggt
     8701 taacgtcacc tggaggcacc aggcactgca tcaacaaagt tcacttcggt gatgaagggt
     8761 aagagaaaat gttgaatgta gctattgaaa accagaatgg gtggaattat agtgcacctg
     8821 cacctcataa agcgggtgcc gggcgtggaa acccgatgat gactactgcg cataaccgcg
     8881 ctcaggcggt ttttttatgc gtaatgcaca gccacattca gattatggtg gggcgtgcag
     8941 ggcagccgaa aggctggccg gtttcggtag tcaccggtat ttccacccct gtacgtctca
     9001 ccacccttat ggtcgtggaa agccttggtg gtgagttatt taaactgact atcgaggctg
     9061 ccatcatggc tactatccct gccctttctc accctgacgt aaccatcgaa aatggacgcg
     9121 ctgtcactac gtctattgcg atcgctgagt tctttggtaa acgccacgaa cgagtgttgg
     9181 ataaaattcg caatctggac tgttcagcaa aattcactga gcacaatttt gtgtcgagcg
     9241 aatataccga ctcaaccagt cgcaaactcc caatgtacca aatcaccaaa aacggcttcg
     9301 ttttcctggt gatgggcttc accggcaaaa aagccgctgc atttaaagaa gcctacatcg
     9361 ctgagttcga tcgcatggag aaagaactgc gccagaataa cgccccgtct cccgacaaaa
     9421 tgattcacgg ggacggacgt accctggtta tccgtctcga cgaacacggc aatatcaaat
     9481 tcactgaaac cgttccggac ggcgcaatgg tctgcaccct ggataccttc cagttttacc
     9541 tggagaaaca aggctggact cttgtaaacc ggagcgcaat taaaaatatg actgtggagc
     9601 aattactaaa aattcattgt tgaggacgcg ataatggaaa cgttattacc aaacgttaat
     9661 acgtctgaag ggtgttttga tattggtgtt ctgctcagta accgggagtt tactgaagat
     9721 gccattaaga tgagaaaata tgagccttat cttctcaatg ataattccat actctccaga
     9781 attgcccttc ttgaacttgg tattttcgga gaacatcaat gacttcagca tttgcactgg
     9841 tgatgacggt ttttcttata acgggtgaat cacagaatgt gattaccgga atttatgcca
     9901 gtaaagaatc ctgcctccag gcaagagacg agcaaaaaat ttctggtgaa tgcctcccgt
     9961 taaaaaaagt atcgctgtac ctgaataacg aaacaccggc tggataaccc agcagccgta
    10021 ttaacgccat acccgttgat taagcatgcc agcaatggca ggggttcgta caaccttaaa
    10081 atagttatga ggtttatcca tgagcactga taaagaagaa ttttcgctat attgcgaagc
    10141 aaaaaatgac aaggtcagaa aacgccttgg gattaaaggg ggtttttact ggactacagc
    10201 aaaaaaatta tctgttgcca tctcacggtg cgttgttgca atggacgatg ctggatttga
    10261 cgaagaggat ttcaaaaaac ctgttcgcgt gaatttgccc attgttaatg acctgccgcc
    10321 tgaaggtgtg tttgataccg aattctgcaa ccgctatgaa aaaggcgggg aagatggcat
    10381 cacaatgata tttatagcgc cttccccctt agttcaggac aaaccagcca gcactgacaa
    10441 taccaacgtc aatggcgaag acatggctga gattgaggat aatatgctcc tgccgatttc
    10501 cggtcaggaa ctgcccattc gctggcttgc gcaacatggc agcgaaaaac cggtaacgca
    10561 cgtttcacgg gaagaacttc aggcattaca tatcgcacga gctgaagaac tgcctgctgt
    10621 tactgccctg gctatttccc acaacacaaa gctgctcgac ccgctggaga ttcgcgacct
    10681 tcacaaactg gtacgcgaca cagacaaagt tttccctaat cccgttaatt ccagtctggg
    10741 gttaatgact gcttttttcg aagcatacct ggacgctgac tataccgatc gaggtctgct
    10801 gacaaaagag tggatgaaag gaaatcgtgt tttacgcatc agccgcacgc catccggcgc
    10861 taatgctggc ggaggaattc ttaccgatcg cggtgaaggt tttgtccacg atgatgcgtc
    10921 agtggaacgt gacgttgccg ctggcgttct ggcccgttca atggacatcg atatttacaa
    10981 tccacatccg gcacacgcca aacgcattga agaaatcgtt tcagagaata agccgccctt
    11041 ttctgttttt cgtgacaaat tcatcgccat gcctggtcac ctggattatt cccgcgcgat
    11101 agtggttgcg tccgtgaaag aagcaccaat tggtatcgag gctactcccc accgtgttac
    11161 cgaatatctg aacaaagtac tgaccgaaac cgaccatgcc aaccctgatc cagaaatcgt
    11221 ggatattgcc tgcggtcgct cctctgctcc aatgccgcag cgtgtaacaa aagaaggaaa
    11281 acaggatgat gaagaaaaac cgcagccatc tggcgcaatg gcagatgaac aggcaacgac
    11341 tgaagcagtg gaaccggata caactgaaca taatcaggac acgcagtcga tggatgctca
    11401 gccacagata aattctgttg atgcgaaata tcagaaactg cgtgcagaac tctatgaagc
    11461 caggaaaaac attccaccca aaaatcctgt cgatgcagat aaattactgg ctgcttctca
    11521 cggagaattt gttgaaggga ttagcgaccc gaatgatcca aaatgggtga aggggattga
    11581 aacccgcgat tctgtgaacc ataaccagca agaaacggaa cagaaaggcc ataaagcgga
    11641 acaacacagt ccaaatgcgc aacaaaacga gccagaaacg aaacagcctg aaccagtagc
    11701 gcaacaggaa ccggaaaaag tctgcacagc ctgcggtcag accggcggcg gcaactgtcc
    11761 tgactgtggc gcggtgatgg gcgacgcaac ataccaggag acattcaacg aagaaagcca
    11821 ggatgaagcc cgggaaaaag atccggagga aatggaaagt gccggactcc cgaacaagga
    11881 gtgcaccgaa ggcgatcaac atgccaatgg caataatgaa acaggcgaga cagcaaatcc
    11941 cttaattaag gtgaacggtc atcgtgaaat cacatccacc agcaggttgt ggcaccatct
    12001 gatgattgac cttgaaacaa tgggcaaaaa tcctgatgcg ccaatagcct caataggcgc
    12061 tgtatttttc gatccacaaa ccggagagca ggggcctgaa ttcagcaaaa taattgatat
    12121 gggtacatgt ggcggcactg tagacataag caccatcgaa tggtggcttc aacgctctgg
    12181 cgaagcccgt gccgccattt tagctgatcg aataccgctt gatgatgcgc ttttacaatt
    12241 acgggaattt atagacgaaa actccggtga gttttttgtt caggtctggg gaaatggagc
    12301 caacttcgac aacgtgattt tacgccgttc atatgaacgg caggagatcc cctgcccgtg
    12361 gcgttacacc aatgatcgcg atgtaagaac gatggtcgct ctggggttgg tgatggattt
    12421 cgatgcccgc aacgtcacca catttgaggg tgaacgccat aatgccctgc acgatgcgcg
    12481 ttaccaggca aaatacgttt cagctatctg gcaaaaactg ttcccaaatc aggctgattt
    12541 ttaatgttca accctgatcg ccgctaaccg catatagtta gcggcggtta tgagatatag
    12601 ctatgagcag cttattttta accgaagatg aattgctaat attaacgggc tgcaaatatg
    12661 caagccacca gcgcaactgg ttaataaaaa atgggcttcc gttctatacc aatcgtagtg
    12721 gaaaaccgat cgtcagtcgg gaactgttta cctgtagaaa cactttacca ccacgtgagg
    12781 ctgaacctga tttcggtgcg atctaatggg aagacgaagg aaaaatcccg aacacgaaaa
    12841 attaccgcca aaggtatatc ccaataaata tagttatgta tggaaaccaa catccagaga
    12901 atctgtaacc ttaactgcaa tcaaggatgg tttagccgca ttatggaaaa aatatgaaga
    12961 aacggttaac catcatgatc acgcaatgac atttgggcgt ttgtgggaaa aatttctcgc
    13021 cagcgcctat tacagcgatc ttagtcccag aacgcaaaaa gattatctgc aacatcaaaa
    13081 aaaactgctg gccgtattcg gtaaggtgct ggccgattct gtaaaaccag agcacatcag
    13141 acgatacatg gacaaaaggg gcgagcagag taaaacgcag gcaaaccatg aaaaaagcag
    13201 catgtcacgc gtttacagtt gggggtatga acgagggtat gtaaaggcta atccttgtgc
    13261 cggtgtaagt aaattcaagg ccaaaaaccg agagcgctat gtgactgaca aagaatacca
    13321 ggccgtatta agcgtggctc ctgtgcctgt ttttatcgca atggaaattg catacctgtg
    13381 cgcagcaagg atttccgatg tgttgtctct gaaatgggaa cagatcggaa acgacgggat
    13441 ctttatccag caaggtaaaa caggaaagaa acagataaaa gcctggactc cccgtctgca
    13501 atcagttata gaaaaagcta aacagctacc caagtcagcc tatgtaatca gcaatcagta
    13561 cggcaaccgt tacatgtata aaggatttaa tgaaatgtgg gttgaggcaa gaaatttggc
    13621 tggacaaata tcagggatcg cgacggattt tacattccat gaccttaaag ccaaggggat
    13681 atcagattac gaaggtagta gccgggataa gcaacttttc tctggtcaca agaccgaagg
    13741 acaggtgcta atctatgata gaaaagtaaa gatctcaccc acactggatg taccattacc
    13801 ccagaatatt ccaacaaaat attccaagta attccaagtg tgattttcat cattgactta
    13861 atgatgtgta agtgattgaa ttctggcgga gagaggggga tttgaacccc cggtggagtt
    13921 gcccccactc cggttttcga gaccggtccg ttcagccgct ccggcatctc tccgttcaga
    13981 tggttgccat gatgccagga aatttggcat tttaacagtc cctgtccgtg caattttgtt
    14041 caagtgacga gtttgcgagc aaaacgatga ttaagtggcc ctggaaagta caagaatcag
    14101 cacatcaaac tgcccttccc tggcaggaag cactatcgat ccccctttta acgtgtctga
    14161 cggaacagga acaaagcaaa ttagtcactc ttgccgaacg ttttttacag caaaagcggc
    14221 ttgttccttt acagggcttt gaactggatt cattaagaag ctgccggata gcacttctat
    14281 tttgcctacc cgttctggag ttaggactgg aatggctgga tggttttcat gaagtcttaa
    14341 tttatcctgc gccatttgtg gtcgatgatg aatgggaaga cgatatcggt ctggtgcata
    14401 accaacgtat tgttcagtca ggtcagagct ggcagcaagg gcctatcgtt ttgaactggt
    14461 tggatataca agattctttt gatgcttctg gttttaacct gattattcat gaagtcgctc
    14521 ataagctgga cacccgtaac ggcgatcgcg ccagcggagt tccctttatt ccgttgcgtg
    14581 aggttgctgg ctgggaacac gatcttcatg ctgcaatgaa caacattcag gaagaaatcg
    14641 aattggttgg cgagaatgcg gcgagcattg atgcttatgc tgccagtgat cctgctgaat
    14701 gttttgccgt actttctgaa tatttcttta gcgccccaga actttttgct cctcgtttcc
    14761 cttcattgtg gcaacgtttc tgccaatttt atcaacaaga tcctttgcag agactgcatc
    14821 gcactaatga tacagactcg ttttcggcga cgaatgttca ttaattaaca actttgcaga
    14881 ttaattaacc aattgaaatg acttatgaaa tttagtgttg acagacaagg taccgctaag
    14941 taatatgcgc cccgttcaca cgattcctct gtagttcagt cggtagaacg gcggactgtt
    15001 aatccgtatg tcactggttc gagtccagtc agaggagcca atttgctgtt ttcatgcatc
    15061 cttgcgaatc attatatgat gttggttcaa caggttagcg tgaaaactct tcccggtgca
    15121 ttttgatttt accctccgca tcgggaaaaa ttggtggtca aatctggggt caggttagtt
    15181 cgataatgga gcgaccccca tatgtccctt accgacgcga aaatccgcac tctcaagcct
    15241 tctgataaac cctttaaagt ctccgattcc cacggtctgt atctgctggt caagccgggt
    15301 ggctcacgtc actggtatct caagtaccgt attaacggta aagaatcgcg cattgcgctg
    15361 ggtgcctatc ctgccgtctc cctctctgat gcgcgacagc agcgtgaagg tgtccgtaaa
    15421 atgctggcgc tgaatatcaa cccggtacag cagcgggctg ctgaacgtgg ctcacgaaca
    15481 ccggataaag tttttaaaaa cgtggcgctg gcgtggcata aaagtaaccg aaaatggtcg
    15541 cagaacaccg ctgaccgtct gcttgccagc atgaacaatc acatctttcc ggtcattggc
    15601 aacctgcctg tatcagaact taaaccccgt catttcattg acctgctgaa aaggatcgag
    15661 gaaaaaggtc tgctggaagt tgcgtcccgc acgcggcagc acctgagtaa cataatgcgc
    15721 catgcggttc atcaggggtt aatcgatacg aacccggcag caaatcttgg cggcgtgacc
    15781 acacctcctg tcagacggca ctatcctgcc ctgccactgg agcggctgcc tgaactgctt
    15841 gaacgtattg aggcatatca tcagggccgt gaactgacgc ggttggccgt cctgctgatg
    15901 ctgcatgtgt tcattcgctc cagtgaactg cgtttcgccc gctggtcaga gattgatttc
    15961 acaaaccgag tctggacgat acccgcgacg cgagaaccca ttattggagt gcgttattcc
    16021 ggccgcgggg ccaaaatgcg aatgccgcat atcgtacccc tctcagaaca gtccatcgca
    16081 attctgaaac agattaagga tatcaccggt aataatgaac tgatcttccc cggcgaccat
    16141 aacccgtata agccaatgtg tgaaaacacg gtcaataagg cactgcgggt gatgggctac
    16201 gacacgaaaa aggatatctg tggccacggc tttcgggcga tggcatgcag tgcgctgatg
    16261 gaatcgggtt tatgggcaaa ggatgcagta gaacgccaga tgagtcatca ggagcgcaat
    16321 accgtgcgca tggcttatat tcacaaggca gaacacttgg aagcccgcaa ggcgatgatg
    16381 cagtggtggt cggactatct ggatatatgc agaaaagcat atgtgtcgcc ttatatgatg
    16441 gttcaggaga accgctgaat tgttagtata acgaagccgt ctccgcttag gtggggacgg
    16501 ctttatttat cagtaataca aaaatgccag tgagcttttt ctcctgaacc tccgttattt
    16561 ctcatgttat aaatttgtaa caaatatttt ttaaactgtc ctgtattgcc acgcaaatct
    16621 atggaaatta caggaaaacg atatgagcag cccccttaaa tcggtgttta aaaaggagca
    16681 cagcgatgag atcagtaacc acagcgtgaa ccccgtcttt tctgaggtag tcagcgcttt
    16741 tatgtcccgt cgccgctttc tgcaaatggg aatggtggcg ggcgctgccg tctcctttcc
    16801 ttatctagtc aagccagaaa acgccttcgc ggcgaaggca aatccctcag cattatctaa
    16861 agcggtgtca ctgggcttca ccagcattcc cgtttcgaca gcagacactg tcaccgtgcc
    16921 ggaaggctat atagcccgtc cgttttatcg ctggggcgat gctgtgggca taaagggtaa
    16981 tctgccggaa tttaaattcg acgctagcaa caccaccgac gaacaggcgg cgcaggccgg
    17041 tatgcaccat gacggcatgg cctggtttag cctgccgcag ggcaaagaga acccggcaca
    17101 cggtctgctg gcgctcaacc atgagtacat tgataacggc atgctgttta aagacggtac
    17161 cgccaactgg gatcttgata aggcccgtaa gggtcagaat gcgatgggta tatcagttat
    17221 cgaggtgaaa aaagataacg tgggctggca ggtagtccgt ccctccagct ttgctcgtcg
    17281 cattaccgtt aatacaccaa tgcaactgag cggtccggcg cgtcatcagg cgttgatgaa
    17341 aaccgccgcc gatccgcaag gcgaagtggt actgggcacc atgcaaaact gcgccaatgg
    17401 taaaaccccg tgggggacgt acctgacctg tgaagagaac tggtcggata tttttgttaa
    17461 aaaagcgccg cgtaacgtgc tggagaagcg ctacggcatt agcgatagcg atgaatccta
    17521 ccgctggaac gaggttgacg aacgtttcag cgtcgataaa acgcccaacg agccgaaccg
    17581 ctttggctgg gtggtggaga ttgaccccta cgatccgacc tccacaccgc gcaaacatac
    17641 cgcattaggt cgctttaagc atgaaggtgc cgccgtgacg ctggcggggg ataatcgcgt
    17701 tgtggtctat atgggcgacg atcagaaatt tgaatacatc tataaattca tctccgagaa
    17761 caaatatgac cctggcgatc gcaaggcaaa tatgcagttg ctggagtcag gcacgctcta
    17821 tgtcgccaga ttcaatgacg acggttccgg cgactggctg ccgctaatct tcggtgaaaa
    17881 tggccttgat caaagcaagg gtttcgacaa tcagggcgat ctgctgatca aaacgcgcct
    17941 tgctgccgat acggtgggcg caacaaaaat ggatcgcccg gaatggatag ccgttgatac
    18001 ccatgcgaaa ggcagcgtgt attgcacgct caccaacaat tccgatcgcg gtaaagaggg
    18061 caaagccccg gttgacgcag ccaacccgcg cgccaacaac cagttcggcc atatcatgca
    18121 ctggcgcgaa gagagggcag accccgcctc agcgaaattt acgtggaata ttctggtgct
    18181 cgccgggcgt actgacagtg acgatcctaa agcgaaaggc tccatgcagg gggcagaatt
    18241 cggcagccct gatgggctct ccttcgatca ccgcggcgtg ctgtggatcc agactgatgt
    18301 ttcctccagt acgattaata aaaaggcgta tgaagggatg gggaataacc agatgatagc
    18361 gaccctgccc ggcaccaatg agtatcgccg ctttttgacc gggccacgcg ggtgtgaaat
    18421 taccggtatc gcctttacgc cggacaaccg tacgctgttt attaatatcc agcatcccgg
    18481 cgaaggcggc gacgacatta ccgatccgag caaccctcgg gctatctcca actggccaga
    18541 ttcaagatca gacggacgac cacgttcttc aacagtcgtt atcactaaat cgaatggcgg
    18601 gataatcggt acctgatgca tctggtgccc ttttgggcac cattttttat ggcaacagct
    18661 tgatcctccc aacattccct aggtatcaaa tatttaatct cgctgttatc tgaaggaatt
    18721 aaccttagcc ttttatgttt tctcttactc gctgaaacga ttatcaattt taacgctacc
    18781 ttaagcctgg cttcgtacat gcacgttcca tatcaaaaaa tcgattaaac gtttacatta
    18841 catttgttat gttataacgt tacaaaaagt aacttattat gtggaatgga tgtcatgcac
    18901 gactactctt tgcgttttgc aaatctggcg ctgggttatg agggtttacc tgcgatacaa
    18961 aatattaccg ggacgataca aaaagggtcc cttacagcca tcatcggccc taacggttct
    19021 ggtaagtcaa cactgcttaa aggtatcgca gggattctgg ctccgttaag tggctcctgt
    19081 accgtagaac caaaggcgcg cattgcctat ctgccacaaa tatcggaatt ggatcgcact
    19141 ttccctgcaa ctgtttcaga tttagtttcc ctaggtttat ggcctgaaag agggttattc
    19201 cgtcatcaca gaattgagga tcgtaaacgg cttactgatg cattgggatc agtaggatta
    19261 gcagggtttg aaaaaagaca gcttagtgca ttatccgggg ggcagcttca acgcgcgctc
    19321 ttcgcacggg tcattcttca acaggcaaac atcatcttgc ttgatgagcc ttttaatgcc
    19381 attgacgcca ctactattga tgacttgctt gtgctgataa accgctggca tgctcagaaa
    19441 cgtactgtct tagctgtcat gcatgatatc ggtctggtta gaaatcattt tccacaggcc
    19501 attttgttaa acggtaaatt agtggcatgg gacgaaacag aacaagttct gcgccatacc
    19561 tcacttctac cggcacagaa tatgggcaca gcacgtgctt tacctgggca acatgaggct
    19621 acacattcat gaactggctt catgaatggg ttctagcacc ttttgtcggc ttcggtttta
    19681 tgcgccgggc gctttatggt tcatttctgc tttgtttaag cgcttgcccg gttggcgttt
    19741 ttttaacatt acgccgtatg agtctggttg gtgatgccat gtcacatgct atcttgcccg
    19801 gtgctgcatt tggcttctta ctctacggat tagatatatt accgatgact attggtgggc
    19861 ttgtcgcagg gctgatagtc gctatttttg caggggcggt ttcccgctta acagtgcaaa
    19921 aagaagacgc ttcaatggct gctttttatt taatctcact cgcagtcggc gtgctcattg
    19981 tatcgcttcg aggctcaagc gtggatctta tgcatgtcct atttggaact gtgcttgcac
    20041 tcaatcacga agcgctgttg ttaattttct ctgtcgcgct gctcacactt ctgctggttt
    20101 ctatattctg gcgcgcactt attgctgagt gccttgaccc tttatttctt cgctccgttt
    20161 cacgattggg atcgccagtc cattttattt tcctggccct ggtggtactg aatcttgtcg
    20221 ctggttttca ggcactcggc actttgctct ctgtcggggt aatggtcttg ccagctgtga
    20281 cggctcgttt ctggacgaga cgtgtagcaa caatgtgcat agcctctgtc ctgataggaa
    20341 tgcttgcatg tataagcggt ttgttatttt catttcattt ttcactacct tcaggaccgt
    20401 caatcatatt atctgtggga attatgtatc ttgtctctgc tgctgccatg atggtgaaaa
    20461 acatccactt aaaaaaactt cgttaatctt ttcactacaa atcgaggaaa ttatttaatg
    20521 agaaaacccg gttttatatc tttatgcatc ctgacgacat caatgctttt tttacttttt
    20581 actttcccgg cgaaagcgaa aattaatgtc atagcaagct tttctgttat tggcgatatg
    20641 gcaaaaaaca tcggtcggga ccgcatcgaa ctgcgtacta ttgtcggccc taatggcgat
    20701 gcccatgttt atgagccctc tcctgctgac gcgatcgcta tgtctaaagc ggatgtcata
    20761 ctggtaaatg gtttacaact tgaaggcttt atttcacggt tgatcgaggc aagtgaaact
    20821 tccgcaccgg taattgaaac aacaaaagga gcaaatattc tccaggaccc ggcaggcgga
    20881 cactatcatt tttatgatgg taaagcggtc tttcatgctg caccgtttga tccacatgcc
    20941 tggcaatcag tagggaattc cagaatatat gtgaaaaata ttgcagaagc cttttgtgca
    21001 caagataaaa gtaattgtga tttttatcag gctaatgccc gtgcttatga tgcaaaactt
    21061 gcggatctgg aaaataaaat tattaagact gtcgctacga tcccacagga aaagcgtact
    21121 gtcgtcgtag gccataatgc attcaaatat tttgaacagg catatggtat acatttcctt
    21181 tcccctcagg gtatatccac cgaatcagag gcctctgccg ctgacgttgc cggcatactt
    21241 aagaaaatta aagaaaataa cgcatcagct gttttttctg aaaatatttc caatccacgt
    21301 ctggttgaac aaattgccag agaagctggc cttaacgtgg ctggcgtgct ttatacagat
    21361 gccttgtcag aaccaggagg accggcttct acctacctgg aaatgatgga acataacgta
    21421 acggcgattg cgacagcggc gagcaatagc cgtaataccc agccataaat acccgccgaa
    21481 cagttttata tatgatggag aacattatga agaaacgctt attatctact tcaatctcaa
    21541 cgctgctttt gggcctttct gtgatgcctg ctttcgcaga tgaagacgta accgcatggc
    21601 gtctgttcat cgccgatcat gataaacccg ttgtgaatgt tattgatgca ctggatggag
    21661 ataagctcgc aacatttaat gttaaaggcc ctgcaaattt atctcgcagc gaaagtggtg
    21721 ccacaatttt cgctatccag ggtagtgctg gcgtagtatc tacaatcgct tcaggcattg
    21781 ctttccatga tcacggagat catgcagaca ttgatattga cgctcccaaa ttattaccgc
    21841 tggaactaac gggtaaaaaa cctggtcatt ttgttgaacg tcagggcaaa attgcccagt
    21901 ggtttgacgg ggaagatagc gcgcaaatcc tgggtgaaag cgccgttctt aaaggtcaga
    21961 agaatataac gaaagtaaat gttgttgcgc cacatcacgg cgttgctgtt ccttacgata
    22021 attatgctgt cgtatccatt cccaaccctg atgatgcgtc aaaacgacct gtcggcgctc
    22081 gcgtggtgga cctgcaaggt aaaaaagtgg gtgatgatgc cttatgcccc ggacttcacg
    22141 gctcagccgg ctcgggtgat acttttgccc tatcatgtga gactggcctc ctgctgatta
    22201 cgcaaaaaaa tgcggcaccg gttatcaggc accttcccta cgcgaaaacg ttgccagagg
    22261 gaagcacatc caccttgatc ggtggtaagg gaatgcaata ttttatcggt aattatgggc
    22321 cagaccggat tattctggtc gatcctaccg aatcggatag cttccgtttg attcagttgc
    22381 ctacccgtcg agtccatttc gttgtagacc cggtacgcgc gaaattcgcc tatgtgttta
    22441 cagaagacgg aaagcttaat caaatagatg tcctgaaagg ggaaatttcg cagtcagtgc
    22501 gtgtcactga tccctattca atggatggcc actggaatga tccacggccg agaatcgcag
    22561 tagctgacaa taaaatttac gtcaccgatc cgttgaaaag taaaattatt gtgttagacg
    22621 ctacttcttt taaaaagacc agtgaaatta gcgtcgaagg gcagccattt aatattgttg
    22681 cagtcggcgg ttcaggaaaa gtacatggtg aacatcacga tcatgaagct catcatcatg
    22741 atgatcacgc tcattaatac ttgtcaaacc taacaacgcc tttcatagta gatctgctga
    22801 tagcagatct actttctggc tttaataatc tttt
//
LOCUS       DAZGXZ010000080        22501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000080 DAZGXZ010000000
VERSION     DAZGXZ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.080"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..443)
                     /locus_tag="ACHKJF_003207"
     CDS             complement(<1..443)
                     /locus_tag="ACHKJF_003207"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0950518.1"
                     /translation="MAFTISCGTRAFRACDFAFVVPVAISIPLLDWWGTVYTEKAQIK
                     PLTWATPKAYSGPHLEIIKVHKCKAPQVSLPLAGLLTTNDSESIEVAMLNHTTHPQGR
                     DSHNLNKYIWRFIALSTAQPRVITIEATSEQEARQQSPAGCVMVFA"
     gene            370..549
                     /locus_tag="ACHKJF_003208"
     CDS             370..549
                     /locus_tag="ACHKJF_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0950519.1"
                     /translation="MATGTTNAKSQALKARVPHEIVNAMESVKESGESTSQFIIASMQ
                     GEIKRRQRRKAKEQE"
     gene            complement(558..992)
                     /locus_tag="ACHKJF_003209"
                     /pseudo
     CDS             complement(558..992)
                     /locus_tag="ACHKJF_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1050..1238)
                     /locus_tag="ACHKJF_003210"
     CDS             complement(1050..1238)
                     /locus_tag="ACHKJF_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0950520.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(1604..2833)
                     /locus_tag="ACHKJF_003211"
     CDS             complement(1604..2833)
                     /locus_tag="ACHKJF_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0950521.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLSDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWMERLGI
                     LAGTHENVTALPAARRK"
     gene            3082..4203
                     /gene="roxA"
                     /locus_tag="ACHKJF_003212"
                     /gene_synonym="ycfD"
     CDS             3082..4203
                     /gene="roxA"
                     /locus_tag="ACHKJF_003212"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP0950522.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4349..5578)
                     /gene="pepT"
                     /locus_tag="ACHKJF_003213"
     CDS             complement(4349..5578)
                     /gene="pepT"
                     /locus_tag="ACHKJF_003213"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP0950523.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            5828..6964
                     /gene="potA"
                     /locus_tag="ACHKJF_003214"
     CDS             5828..6964
                     /gene="potA"
                     /locus_tag="ACHKJF_003214"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP0950524.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            6948..7805
                     /gene="potB"
                     /locus_tag="ACHKJF_003215"
     CDS             6948..7805
                     /gene="potB"
                     /locus_tag="ACHKJF_003215"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP0950525.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            7802..8596
                     /gene="potC"
                     /locus_tag="ACHKJF_003216"
     CDS             7802..8596
                     /gene="potC"
                     /locus_tag="ACHKJF_003216"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP0950526.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            8593..9639
                     /gene="potD"
                     /locus_tag="ACHKJF_003217"
     CDS             8593..9639
                     /gene="potD"
                     /locus_tag="ACHKJF_003217"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP0950527.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            9697..10158
                     /locus_tag="ACHKJF_003218"
     CDS             9697..10158
                     /locus_tag="ACHKJF_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP0950528.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            10155..10943
                     /gene="ycfZ"
                     /locus_tag="ACHKJF_003219"
     CDS             10155..10943
                     /gene="ycfZ"
                     /locus_tag="ACHKJF_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0950529.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(11082..11903)
                     /gene="cobB"
                     /locus_tag="ACHKJF_003220"
     CDS             complement(11082..11903)
                     /gene="cobB"
                     /locus_tag="ACHKJF_003220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP0950530.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(11919..12830)
                     /gene="nagK"
                     /locus_tag="ACHKJF_003221"
     CDS             complement(11919..12830)
                     /gene="nagK"
                     /locus_tag="ACHKJF_003221"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP0950531.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(12859..14103)
                     /gene="lolE"
                     /locus_tag="ACHKJF_003222"
     CDS             complement(12859..14103)
                     /gene="lolE"
                     /locus_tag="ACHKJF_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP0950532.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(14103..14804)
                     /gene="lolD"
                     /locus_tag="ACHKJF_003223"
     CDS             complement(14103..14804)
                     /gene="lolD"
                     /locus_tag="ACHKJF_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP0950533.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(14797..15996)
                     /gene="lolC"
                     /locus_tag="ACHKJF_003224"
     CDS             complement(14797..15996)
                     /gene="lolC"
                     /locus_tag="ACHKJF_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP0950534.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            16258..17331
                     /gene="ycfT"
                     /locus_tag="ACHKJF_003225"
     CDS             16258..17331
                     /gene="ycfT"
                     /locus_tag="ACHKJF_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP0950535.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWSIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATLMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQQFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLGYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAISYSR"
     gene            17459..20905
                     /gene="mfd"
                     /locus_tag="ACHKJF_003226"
     CDS             17459..20905
                     /gene="mfd"
                     /locus_tag="ACHKJF_003226"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP0950536.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            21049..22011
                     /gene="ldtC"
                     /locus_tag="ACHKJF_003227"
     CDS             21049..22011
                     /gene="ldtC"
                     /locus_tag="ACHKJF_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP0950537.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(22094..22351)
                     /gene="bhsA"
                     /locus_tag="ACHKJF_003228"
                     /gene_synonym="ycfR"
     CDS             complement(22094..22351)
                     /gene="bhsA"
                     /locus_tag="ACHKJF_003228"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP0950538.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         5502 a         5820 c         5593 g         5586 t
ORIGIN      
        1 gcaaatacca tcacgcagcc agccggagat tgctggcgtg cttcctgttc gctggtggcc
       61 tcaatggtaa tcacgcgcgg ttgtgccgtg ctcagggcga taaaacgcca gatgtattta
      121 ttcaggttgt gcgagtcccg cccttgcggg tgtgtggtat gatttaacat agctacctcg
      181 atactttcgc tatcgttggt ggttaggagc cctgcgagtg gtaacgacac ttgcggggct
      241 ttgcatttat gcaccttgat aatctcaagg tgtggcccac tatatgcttt aggtgtggcc
      301 cacgtcaagg gttttatttg tgctttttct gtgtatactg tcccccacca atccaacaga
      361 ggaatagaaa tggcaacggg tacaacaaac gcaaaatcac aagctctaaa ggctcgtgta
      421 ccacacgaaa tagtaaacgc catggaatca gtgaaagaat caggcgaaag cacatcacaa
      481 ttcatcattg cgtcaatgca aggcgagatc aaacgccgcc agcgccgcaa ggccaaagag
      541 caggagtaac catcaccagc gccgtggtgt aaggtattac ggcgcattgc tatgcaggac
      601 aacacaatga ccgataaaga attgaccaaa acattatcac cggcacggaa aagacggcgc
      661 agaaagatag agcatgaatc agaaagattc gcgccatgtg cttttgccct tgagcaattc
      721 cttaaagagt acagggaaaa gcgctcattg caggtatggc aacgaactga accagactga
      781 tagcattgcc caccagcctg atagcgacta tcattgccgt tcttatgttt gggatcacac
      841 acacaaacgg cgcagcgggt tatctgttca gaaaggcggc tccatttcgg ggccgctttt
      901 tttatgtctg aaaaacccca attttgttgt ttttcagttt caccagggcg aacgaatccc
      961 cgcccacgtt ctggcgtata ttcaatcttc atggttatag ctctgtgttc agatgattga
     1021 tgtgtggcgg ctgcgtgccg ccagcgtgat taatgaactg ccttgcagct atccttccag
     1081 gccagaacct cggataaaga ccagccaacg gaacgaccgc caagtttacg acgtgatggg
     1141 aattgtccgg ccttttccag gcggtagcga cacgtacggc taaggccggt tagtttttcg
     1201 cattcttttt cacgtataaa ccgatcagtg cttaacacta ttgcccccct ttcgtttctt
     1261 aaagagtcat caggtgtctt attgtgtcgt attgttcccg ctagagtgat gaatggcaaa
     1321 tattgaggat gtatgattta cagaaaatga aacagtaaga ataaaatctt ttaaattcat
     1381 gttaatacaa aggcataaaa tattgtttca tgcctttttt ctcacgcgtt aaagcgttat
     1441 tcgctaatgt ataaaaaaac agtcacacaa ataaaatcag tcacttataa acctgtgtac
     1501 tttttcgcct cttgttcgtg gttgttccac gttgtttcac attgttcgcc gttgtatctg
     1561 tgtgcttatc cagtatgcgc atactgaaaa aacacgaaaa aaattatttt cttctggctg
     1621 ctggtagtgc ggttacgttt tcatgtgttc ccgccagtat ccctaaccgc tccatccaca
     1681 tatccagcgc attgcgttta gcatccagat aacgggaatg attataaact cgctgcattc
     1741 ctggcatctg gtggcctgta agctgctcca cgacgtgagg atccacgcct aaatcgctca
     1801 gcatcgttgt aaaggtgcgc cggatgtcat gtagtgacca gtgagggtga tttagcctcc
     1861 tgtgcgctaa tctgccgtac tgcgacacgc ttgtttcctg tttcacttcc cccagcaata
     1921 agcccgtgtg cctgttctgc tccaccagct gcgtgacgaa cggcagtatt gcttccggta
     1981 tgggccggaa tattgccacc ttcgttttgc tgtgttcttt cggcacggtc cagagcattt
     2041 cggtaaaatc ccactcgctg atctccgata acctcagttc taccgtccgg catccgaaca
     2101 caatcaggag gcggattaac gcgatgtagt agggggagaa tatttttttg tccagtgcct
     2161 gcaataattc gcccagttct ttggtgctta agacacgctc gcttatatcc ggttttttcc
     2221 caacgtccgc cacactcata tcatcaagaa cgttgctgat tgcatagcgc cgcctccggc
     2281 agaacttaag cgcctgtttg cacgtctgta gcaagaatcc ggcagtaaca ggcgttcgct
     2341 ttgccacctg gtcaaaacag gccagccagt gccgtagctc gcatttatcc agcggcatag
     2401 caccaatgtg ctgtattacg tgattattaa ggcgcttttt cagggcggca taatccacgc
     2461 ggttttcctt tgcgtacgac tcaagccagt aggtgagcgc atcgccaacc gttaccggct
     2521 ttaacgcttc ctgtacggtg taattaagct catgacgtgg atttttcccc tctgccagcc
     2581 atgcgcgaca ctgggcggct ttttccctgg ctgatttcag gctcagatca ggataatttc
     2641 ccagcttaat gcgttccggt ggtgcccccc ttcccgttcc ggccctgtaa gtgaaatacc
     2701 aggttaaaag gccactggtt gaatgcctga cgctcaggtt tccaccgtca ttaagaaagg
     2761 ctgttttttg ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc agtttgttaa
     2821 gtgctctgct cataattttg attccggtca tcacaattaa ctacaccatt aactacaccg
     2881 atcgttgcac aaagggctac aacgtgaaac aagctggaac aaggaaaatc acaaatctgt
     2941 ttataatcaa aaacataatg aacaatctga aacattatga aacggcaaaa aacactaatt
     3001 gataaaatat gtccatactt cacgcattac gttaagcatc cgttataatc ggttgcagat
     3061 accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc cgattttctt
     3121 gaacgtcact ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa ttttattgac
     3181 ccgatctctc cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga cagtcgactg
     3241 gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag ctacgatcat
     3301 ctcggtgaaa ccaactggtc attactggta caggcagtga accactggca tgagccgacc
     3361 gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga tctgatgatc
     3421 tctttttctg taccgggcgg cggcgtcggc ccgcatctcg atcagtacga cgtgtttatc
     3481 attcagggta ccggtcgtcg tcgctggcga gtgggcgaaa agctgcaaat gaaacagcac
     3541 tgtccgcacc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga tgaagagctg
     3601 gagcctggcg atattcttta tattccgcca ggattcccgc atgaaggcta tgcgctggaa
     3661 aatgcgatga actattccgt gggttttcgc gcgccgaata cgcgggaact gattagcgga
     3721 tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga tccggatgtt
     3781 ccacctcgcg ctcatcctgc ggatgttctg ccgcaagaga tggataaact gcgtgagatg
     3841 atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga gtttatatcc
     3901 cagtcacgtc atgaactgga tatcgcgccg ccggaaccgc cttatcagcc agatgaaatc
     3961 tacgatgcgc tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg cgtattgcgc
     4021 attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg tccggcactg
     4081 gatgcactcg ccagcaacat tgcgctgact gcggagaatt ttggcgatgc gctggaagat
     4141 ccgtcattcc tcgcaatgct cgcggcgctg gtaaatagcg ggtattggtt cttcgaaggg
     4201 taagtttgga tttaagccgg atgtggtgct gattgcgcct tatcgtgcct acatgcactg
     4261 aacgcctgta ggcacgataa gtatgtggac cagtgtcaat ataacggttt catcccggta
     4321 attggcagtc cacatacgca tcgggcaatc acgacttccg ttgcgccgtt aactcggcaa
     4381 tacggacgat cacctgcacc gctttttcca taccttccag agtcacaaac tcatgcttac
     4441 catgatagtt gtaaccgcca gtgaacaggt tcgggcaagg taatcccata aacgacaact
     4501 gcgcgccgtc ggtaccgccg cggatcggtt tcagttccgg ttcaatatcg cagtcacgca
     4561 tcgcctgctg ggcgatatcg agaatatgcg gatgctcaac cactttctcg cgcatattgt
     4621 agtaactgtc ttcaatcacc aattcaatgt agcaatcagg atgtaaccct ttgcccactt
     4681 ttttggcgat ctccatcatt ttacgtttac gcgcttcaaa ctgtttacgg tcgaaatcac
     4741 ggatgatgta atgcatatcg gcccgttcaa cggtgccttt catgctcgcc aggtgataga
     4801 aaccttcata gccttctgtc atttccgggc tttcatccgc cggaacttcc gcatgaatac
     4861 gtgccgccag cgacagcgca tttaccatca ctcctttcgc cgtgcccggg tgaacattgt
     4921 taccgacaat tttgatattg accgacgcgg cgttgaagtt ttcaaactcc agttcgccta
     4981 cgccaccacc atcaacagtg taagcccagc gggcatcgaa ggcgtcaaca tcaaaatgtt
     5041 tcgccccttt gcccacttct tcatccgggg taaaggcgac gcgaatatca ccatgcggaa
     5101 tgtttttctg ttgcaatacc gccagcgcgg tcatgatttc tgcaataccg gctttgtcat
     5161 cggcacctaa caaggtttta ccatcggtag taatcagcgt ctgacccagt agctgatgca
     5221 gcaccgggaa cataaccggt gataaaactt catcgccgat acctagcgca atatcgccac
     5281 ctcgatagtt ttcaacaatt tgcggattca catttttgcc gctgcaatcc ggtgaggtat
     5341 ccacatgaga aataaagcca atcgccggga tattgccagg gacgttagcc ggtaacgtcg
     5401 ccatcaaagt gcccttctca cttaaggtca cattgataag ccccatctct tcgagctgct
     5461 ctttcagcag atgcaataac ttccattggc cttccgtgct gggaacctgt ctcacccctg
     5521 cttttgattg ggtatccaga gacacgtagt tcaaaaatcg ctcaagtagt ttatccatgt
     5581 agtcaccctc actttttgtg acaacattat taagaagcaa gaaaagacaa atattgcgtc
     5641 aggtcacttt tacccctgca agcgggaata tttatcagca ttaccttaat gaatataaag
     5701 ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc
     5761 cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg
     5821 cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt
     5881 gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct
     5941 gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac
     6001 cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa
     6061 cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta
     6121 cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa
     6181 aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcagctgga
     6241 aaccttcgct caacgcaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat
     6301 tgcccgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct
     6361 ggattacaaa ctgcgcaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg
     6421 cattacattc gtcttcgtga ctcacgacca ggaagaagca ctcaccatgt cagacaggat
     6481 tgtggtgatg cgcgatggtc gcattgagca agacggcacg ccgcgtgaaa tctacgaaga
     6541 gccgaaaaac ctgtttgttg ccgggttcat tggcgaaatt aatatgttta acgccacggt
     6601 gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat
     6661 ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga
     6721 cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg
     6781 tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat
     6841 ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact ctctcgacca
     6901 aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa
     6961 gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc
     7021 tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca
     7081 aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc
     7141 tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctatc
     7201 catttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc
     7261 tgattgttcc gttctggacc aactcattga ttcgtatcta cgggctgaaa attttcctca
     7321 gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaattc
     7381 gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg ctaccgttta
     7441 tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc
     7501 gcgatctcgg tgccagcaag ttacagactt ttatccgtat cattattcca ctgacgatgc
     7561 cgggaattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtat
     7621 ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtacagttcc
     7681 tcaatattcg tgactggcca tttggtgcag ccaccagcat tacgctgact atcgtaatgg
     7741 gcctgatgtt gctggtttac tggcgcgctt ctcgtctgct gaataaaaag gtggaactcg
     7801 aatgatcggt cgactgcttc gcggcggttt tatgaccgct atctacgcgt acctgtatat
     7861 cccaatcatt attttgattg tgaactcctt taacagctcg cgctttggca tcaactggca
     7921 gggttttacc accaaatggt atagcctgct gatgaacaac gacagcctgt tacaggcagc
     7981 gcagcattca ctaacaatgg cggtgttttc ggcgacgttt gctacgctta tcggttcact
     8041 gacggcagtt gcgctgtacc gttatcgctt tcgcggtaag ccgttcgtta gcggaatgct
     8101 gtttgtggtg atgatgtcgc cagatattgt gatggcgatt tctctgctgg tgctgtttat
     8161 gctgctgggt attcagcttg gcttctggtc gctgctgttc tcgcatatca ccttctgcct
     8221 gccgtttgtg gtggtgacgg tgtattcgcg cctgaaaggt tttgacgtgc ggatgctgga
     8281 agcggcgaaa gatctcggtg ccagtgaatt taccattctg cgaaaaatca ttctgccact
     8341 ggcaatgcct gcggtggcgg cgggctgggt gttaagcttt accctgtcga tggacgatgt
     8401 agtagtttct tcgttcgtca ccggaccaag ctatgaaatt ctgccgttaa aaatttattc
     8461 gatggtcaaa gtcggcgtat cgccggaagt taacgcgctg gcaaccatat tactggtgct
     8521 gtcgctggtg atggtaattg ccagccagct tattgctcgt gataaaacga aaggtaacac
     8581 aggggacgtt aaatgaaaaa atggtcacgc cacctgctcg cggcgggtgc tctggcactg
     8641 ggcatgagcg ccgctcacgc cgatgacaac aatacgctgt atttctacaa ctggaccgag
     8701 tacgtgccgc caggactgct tgaacagttc accaaagaaa ccggtattaa ggttatctat
     8761 tcgacttacg agtcgaacga aaccatgtac gccaagttga aaacgtacaa agacggtgcc
     8821 tacgacctgg tggttccttc aacctattac gtcgataaaa tgcgtaaaga agggatgatc
     8881 cagaagatcg acaagtcgaa gttaagcaat ttcagcaacc tcgatccaga catgctcaac
     8941 aagccgtttg acccgaataa cgactactcc attccgtata tctggggggc gacggcgatt
     9001 ggcgttaacg gtgatgcggt agatccgaaa tccgttacca gctgggccga tctgtggaag
     9061 cctgagtaca aaggtagcct gctgctgacc gacgatgccc gtgaagtgtt ccagatggcg
     9121 ctgcgcaaac tgggctactc cggtaacact accgatccga aagagattga agctgcatat
     9181 aacgagctga aaaaactgat gccaaacgtc gcagcgttta actccgataa cccggctaac
     9241 ccgtacatgg aaggcgaagt taacctcggc atgatctgga acggctctgc tttcgttgca
     9301 cgccaggcgg gtacgccaat tgacgtggtg tggccgaaag aaggcggcat tttctggatg
     9361 gacagcctgg cgatcccggc aaatgccaaa aacaaagaag gcgcgctgaa attgatcaac
     9421 ttcctgctgc gcccggatgt ggcaaaacag gttgccgaaa ctatcggtta tccaacgcca
     9481 aaccttgcgg cgcgtaagct gttaagcccg gaagtggcga acgataaaac gctctacccg
     9541 gatgctgaaa ccattaaaaa tggtgaatgg cagaatgacg ttggcgcagc cagcagcatt
     9601 tatgaagagt attatcagaa gctgaaagca ggacgttaat tcagcaaacg ggcggtggta
     9661 ttaccgcccg ttttttagtt agcaaggatg tggctaatga gtcaggacag taaagtttta
     9721 tcccgcgctt ttctcggtat cggtctgata ttgctcgtta tttctgcaat catcttttat
     9781 taccatttta cttttaccaa aagcgcggtt catacagaag gaatcattgt tgatgccgtt
     9841 tggtacaaca atcactcaaa cgacgtcgat gataatggtt cctggtatcc agtggttgcc
     9901 tttcgcccta cgccagatta cacgctaatt ttcaattcga atatcggtag tgatttttat
     9961 gaagatagcg aaggtgacag agtcaatgtc tattatccgc cagggcatcc agaacaagcc
    10021 gaaataaaca atccctgggt taattttttt aaatggggct ttgttggcat agcaggcatt
    10081 attttcggta gtgtcggtct gataatcacg ctcccctcca ccaaaaaatc tcgcaggaaa
    10141 cggaagtccc gcccatgaaa aaattcatta tactgctcag tttactgata ctcctgcctc
    10201 tggtagcaac cagtaaacca ttgattccaa taatgaagac actttttacg gatgttaccg
    10261 gtactgtacc ggatgctgaa gagatcgcac gtaaagccga gctttttcgc caacaaacgg
    10321 gaattgcacc gtttattgtg attttacccg acattaataa tgaagccagt ctcagacaaa
    10381 atggtaaagc gatgctggcg catgcgtcat cttcattgag taatgtaaaa ggaagtgttc
    10441 tgttactatt tactacccgc gaaccacggt taattatgat caccaacggc caggttgaaa
    10501 gtggtctgga cgataaacat ctcggccttc tgatagaaaa tcacacgctg gcttatttaa
    10561 atgcagatct ctggtatcag ggaatcaata atgcattggc tgttctacaa gcacagatat
    10621 taaaacaacc gacgccgcca ctgacgtatt atccgcatcc aggacaacag catgaaaatg
    10681 ctccccccgg cagcaccaac acattaggtt ttatcgcctg ggctgcgacg tttatcttat
    10741 tttcctggat cttttgctac acgactcgtt ttatttatgc gttaaaattt gcagtggcga
    10801 tgactattgc caatatgggt tatcaggctt tatgtttgta tatcgataat agctttgcga
    10861 ttaccagaat atcgccactc tgggcagggc taattggtgt ctgtacgttt attgccgcat
    10921 tgttattgac gagtaaacga taaaagaggc cggaaaagtg cgcgtgtatt atgccggatg
    10981 gggcgcgaac gccttatccg gcccacaaaa cccgcaaatt caattaattg cgtccccttg
    11041 taggcctgat aagcgtagcg catcaggcaa tgcttcctgc tttataatcc cttcagcaac
    11101 ttttcaacaa actcaggcac cacctggctt gccgggccgt aatatttctc ggcaaattca
    11161 ttaccgacct gacttggttc gagattcagt tccacggtgt gcgcgccatg cagtttcgct
    11221 tcgtgaacaa acccagccgc cggataaaca tgcccggaag taccaatggc aatgaaaata
    11281 tcagccatcg acaacgccat ataaatttca tccatgccga gtggcatttc gccaaaccaa
    11341 acgacgtgcg ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta
    11401 acgtctccgg tccagtcgag aacctgacca ctttgcgaac aacgaacttt cagcaattcc
    11461 ccatgcatat gaatgacatt ggtattacct gcgcgttcat gcaggttgtc tatattctgc
    11521 gttaccagca aaaagcgatc gccgagggcg tcttgcagtt tagccagcgc aagatgcgcg
    11581 gcgttaggct gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgcttgc
    11641 accagttcag gatcgcgatc gaaaccttcc ggcgttgcca catcttcaac ccgatgttct
    11701 tcccacaggc catctgcggc gcgaaaggta cgaatacctg attccgcaga aatccctgcc
    11761 cctgtcagta cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa
    11821 aaaatacgct gacgcaaacg ctcgcgcagg cggcgtttat ttttacgaaa acgacttaac
    11881 cgatgacccc gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag
    11941 gccgcaccac gcattcctcc cgcatcaccg tggcgcgcgc gttcaatgcg cggaacacga
    12001 gctacaggta agagatgacg aggcagcctg tccgccagtt gcgttgtgat tgccgggaaa
    12061 ttcgataagc caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc
    12121 agacaaaccg ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct
    12181 tgatcataaa gcgcaataat ttcgggagcc tgcaacggtt gatgataata gtgttgatac
    12241 agccacgcaa aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg
    12301 cagcggcgta acgggaaatc caaccccatc atggttaacg catcaaccgg cagacgcata
    12361 tggccaaact cgccggtaat gtagctttta ccggtaatcg gtttgccgtt gaaaatcagc
    12421 ccgccgccaa cgccggtgcc gagaatcaac cccatcacca gtggatattg agtaaattca
    12481 tcatcccagg cttctgaaag ggcaaaacag ttggcatcgt tatcaaggcg tacatcgcga
    12541 tcaagacgtg cgctcaggtc ggcacgcagc ggtttaccgc tggcagcggg gacattggcg
    12601 gcatacagcg taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct
    12661 ttacagccaa aacgttggtc agcttcagct accagctcac acactgcatc taaaaatgcg
    12721 tcatagctgt cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca
    12781 aacacgccaa gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct
    12841 tgttttaatg tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc
    12901 gcgccgcgcc ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta
    12961 gaagacgtcc agccagtgca attccgatgg caggaaatca ataaaataga tatcgctgga
    13021 gaggaactga tgaccgatca acttttcaat ccactcaata atcggggtaa gttgcagtga
    13081 aaccaccaca ccgataatca ccccacacag gctgccgaac agccctgcca gcaatccata
    13141 ccagacaaag atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat
    13201 atcgccactc ttgtctttca ccgccatcac taatgtggag acgatgttga aacaggccac
    13261 gccaatcacc agtaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata
    13321 catatagccg taagtaccaa tccaactttt aatataaaca tagctgttgg tcacttcacc
    13381 cgcatcgcgt accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga
    13441 cacgctggaa cccatatcaa gatattgttg ggcatccgcc agcgggatca tggcaaaact
    13501 gtgatcgagt tgaccactca actgcaaaat accggcaacg tgcaaacgca cacgttttgg
    13561 ctgcatcagt ttatgctcgg gattcgagtt ggggatcata atcgacaccc aatccccctg
    13621 cttcactttc agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt
    13681 aaaattgcgc cacgcatcgc cctggacaaa cgagggtaat gcgctcagac gctgttcctg
    13741 ttgcgggtta acgcccttca cctggattgc gcgaagattc acgccacttt ccaccagccc
    13801 ggtgaaattg atatacggcg cggcagcggc aatacccggc actttttgca cgtgatccag
    13861 tgcttcctgc cagttagtcc acggctgatc caccgcctcg atttcaccat gcggcaccac
    13921 cgccagaata cggttgttca gttcgcgttc aaagccgttc atcgcgctta agccgacgat
    13981 caataccgcc acgccgaggg caatgccaat ggtagaaata acggagatca gcgacaccat
    14041 gccgccgcgc cgccgtccgc gactaaaacg caggccaatt aataacgata aaggcatcgc
    14101 cattactccg cccccatcag gctcagttcc gccgtcagac gaccatcacg catttctaac
    14161 tggcggctca tacgtttcgc cagttgcagg tcgtgagtaa ccaccaggaa ggcggtgccc
    14221 tgcaagcgat tcaattcccc aagcaactgg aagatgctgt cggcgttacg cgcatcgagg
    14281 ttaccggtag gttcatccgc cagtaccagg cgcgggttat tcaccagcgc acgggcaatc
    14341 gccacacgct ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgatcc
    14401 agccccaccg cttttaacat ctcaagtgca cggctgttga tttcagcggg ctttttcttg
    14461 ccaatcagca gcggcatagc cacgttttcc agggcggtaa aatccggcag caggtggtga
    14521 aactgataaa taaagcccag cttctggttg cgcagttctg ctttcgccgc cgaagacagt
    14581 ttgctcatcg gttgaccgtt aaagatcaca tcgccggagg ttggtgtatc cagcccgccc
    14641 agcaggtgca gcaaggtact tttaccggaa ccagagctac cgacgatcgc catcatttca
    14701 ccttcgccca cgctgaaact gacattgtgc agtacatcgg tttgcacact gccttcctga
    14761 tagcgtttgc acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc
    14821 gggttgtgtg gcggcagcgc gccatgaagg gtaaagcgta gacagcagcg cgatagccat
    14881 cgccaccagc gcaataacaa tgacctgtaa aggttcgata gccaccggca gcgccgcgcc
    14941 atcaagcagg acgccgatta tcggcatcag attatttaac tggctggcaa gcagtgcgcc
    15001 aagcgccgca ccgaggatcg caccgataat cccggcgctg gccccttgca ccataaagac
    15061 catcatgatt tgtcgcggag ttaagccttg cgtttgcagg atcgcgactt cgccctgctt
    15121 ctccattacc atcagcccta gtgaggtaat aatgttaaac gccgcaacgg cgacaatcag
    15181 gctcagcagc aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt
    15241 acggtcacgc cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaaagagtc
    15301 aactttcagc ggctcatcca gccacaaacg ccagccggta atattgcctg ccggataacg
    15361 catcagacgc gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt
    15421 ggcggcgaaa gtgccaatca cattgaacag gcgctggctt ggaatacgcc ccatcggcgt
    15481 gaactggctg gcagatggta ccatcacgcg gatttgatca ccgcgattaa cgcctagctg
    15541 tgaggcaagt tgttcgccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt
    15601 cacattgacc agatacggtg taagtggatc tttttgcgcc gggtcgatac cgagcatcac
    15661 cccgaccgcc acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac
    15721 gcgattaacg ccgtccagtt tgactgccgt ttctgggagt tgctgcgggt taagagagcc
    15781 atgctcagaa gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg
    15841 ctcaaagccg ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc
    15901 gatggtagaa agccaggaga cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta
    15961 acgcaggcca ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca
    16021 aagacacgag tatataagcg aaagcaagat gggtaaacgt ctgaactgta agtgatcgtc
    16081 agggctaatc tgaaaagccc ccgtgaatca atcggctaaa ttgccatgtt ttatcatcaa
    16141 tcttgccgcc tgatcttaat ctgaaaaatc atccaataca gacagatacc cattacacat
    16201 agggtcataa cccgcaggat caaccgcatg taaattaaaa aagagcgtgt tgtcctgatg
    16261 aaacaaaaag agctatggat taaccagatc aaagggttat gtatttgtct ggtggtgatt
    16321 tatcactcgg tcattacctt ttatccgcat ctgaccactt ttcagcatcc gttatcggaa
    16381 gtcctgagca aatgctggat ctatttcaat ctttaccttg caccctttcg tatgccggtt
    16441 tttttcttta tttccggcta tttgattcgc cgctatatcg acagcgtacc gtggggaaat
    16501 tgtctcgata aacgcatctg gagcatcttt tgggtgctgg cgctttgggg cgtggtgcag
    16561 tggctggcgt taagtgcgct taaccagtgg ctggcacctg agcgcgattt aagcaacgct
    16621 tccaatgccg cgtatgccga ttccaccggt gagttcctgc acgggatgat cacagccagc
    16681 accagcttgt ggtatctgta tgcgttaatt gtctatttcg tggtatgtaa aatttttagc
    16741 cgtctggcgc tgccactatt cgccttgttt gtactgctga gtgtggcggt taatttcgtt
    16801 cccacgccgt ggtggggaat gaacagtgtg atccgcaatt tgccttatta cagccttggc
    16861 gcatggtttg gcgcaacatt aatgacctgt gttaaagagg tgccgttgcg ccgccatctg
    16921 ctgatggctt ctttgcttac cgttctggcg gtcggtgcct ggttgtttac tatctcgctg
    16981 ctgttgtcgc tggtatcgat tgtggtgatc atgaagctgt tttatcaata cgagcaacaa
    17041 ttcggtatgc gctccaccag cctgctgaat gtgattggct ccaacaccat tgctatctac
    17101 accacccatc gcattctggt tgaaatattc agcttaactc tgcttgcgca aatgaacgca
    17161 gcacgctggt cgccgcaagt cgaactgaca ctcctgctgg gttacccctt tgttagtttg
    17221 ttcatctgta ctgttgcggg cttgctggta agaaaacttt cacagcgcgc attcagcgat
    17281 ctgttgttct ccccgccttc tctgcccgcg gccatcagtt actcccgcta agccatcgcc
    17341 ccagccgggg cgattcaatt tgctaaacca tgtcgattac ggataatgaa cagcattgct
    17401 tatcagagat atgcccccat atgttgaggc atatcctaac gagaatctga caaccgttat
    17461 gcctgaacaa tatcgttata cgctgcccgt caaagcgggt gagcagcgtc tgctgggcga
    17521 gttaaccggc gctgcctgcg cgacgctggt agcggaaatt gccgaacgtc acgccggtcc
    17581 ggtggtactc attgcaccag atatgcaaaa tgctctgcgt ttgcatgatg aaatcagcca
    17641 gtttaccgat caaatggtga tgaatctggc ggactgggaa actcttccct acgacagttt
    17701 ttcgcctcat caggacatta tctcctcgcg cctttccacc ctttaccagc taccgacgat
    17761 gcagcgtggc gtactgattg ttccggtgaa tacgcttatg cagcgcgttt gcccgcacag
    17821 ttttcttcac ggtcatgcgc tggtgatgaa aaaaggtcag cgtctgtcac gagatgcatt
    17881 aagaacccaa ctggacagcg ccggttatcg ccatgttgac caggtaatgg agcacggcga
    17941 atacgccacg cgcggcgcgt tgctggatct cttcccgatg gggagtgagc tgccttatcg
    18001 tcttgatttc tttgatgatg aaatcgacag cctgcgggtg tttgacgtcg acagccagcg
    18061 cacgctggag gaagtagaag cgatcaatct gctgcccgcg cacgaatttc cgaccgataa
    18121 agcggcaatt gaactgttcc gcagccagtg gcgcgatacc ttcgaagtga agcgcgatcc
    18181 ggaacatatt taccagcaag tgagtaaagg cacattacct gccgggatcg agtactggca
    18241 gccattgttc ttcagcgaac cactgccgcc gctgttcagt tatttccctg ccaatacctt
    18301 gctggtgaat actggcgatc tggaaaccag tgccgaacgt ttccaggctg acacgctggc
    18361 gcgttttgag aatcgcggcg tcgatccgat gcgcccgctg ttgccaccac aatcgctctg
    18421 gctgcgggtg gacgagctct tctcagagct gaaaaactgg ccccgggtgc agctaaaaac
    18481 tgaacattta ccgacaaaag ccgcgaatgc caatttaggt ttccagaaac tgccagacct
    18541 ggccgttcag gcacaacaaa aagcgccgct ggatgcgctg cgtaagttcc tcgagacttt
    18601 cgacggtccg gtggtgttct cggtagaaag tgaaggtcgc cgtgaagcgc tgggtgaact
    18661 gctcgcacga attaaaattg ctccgcaacg cattatgcgt cttgatgaag ccagcgaccg
    18721 tgggcgttat ctgatgattg gcgctgccga acatggtttt gtcgataagg tgcgtaatct
    18781 ggcgctgatc tgcgaaagcg atctgctcgg tgaacgtgtt gcccgccgtc gtcaggattc
    18841 tcgccgcacc atcaaccccg atacactgat ccgtaacctt gcggagctgc atattggtca
    18901 gccggtggtc catctggagc acggtgtcgg gcgctatgcc ggaatgacca cgctcgaagc
    18961 gggcggcatt actggcgagt atttgatgct cacctatgcc aacgacgcca aactgtatgt
    19021 tccggtgtcg tcactgcatc tgattagccg ttacgcaggt ggcgcggaag aaaacgcccc
    19081 gctgcataaa cttggcggcg atgcgtggtc acgcgcgcgg cagaaagcgg cggaaaaagt
    19141 gcgtgatgtg gcggcggaat tgctggatat ctacgcgcaa cgcgccgcca aagagggctt
    19201 cgcgtttaaa cacgatcgtg agcagtatca gttgttctgc gacagcttcc cgtttgaaac
    19261 cacgccggat caggcgcagg ccattaatgc ggtacttagc gacatgtgtc agccgctggc
    19321 aatggatcgt ctggtgtgcg gcgatgttgg ctttggtaaa acagaagtgg cgatgcgcgc
    19381 cgctttcctg gcagtagata accacaagca ggtggcggtg ttggtgccta ccacccttct
    19441 cgcgcagcag cattacgaca acttccgcga ccgtttcgcc aactggccgg tacgtatcga
    19501 aatgatctcc cgtttccgca gcgccaaaga gcagacgcaa atccttgcgg aagtggcgga
    19561 agggaaaatc gatattctga tcggtacgca caaactgctg caaagtgacg tcaagtttaa
    19621 agatttaggc ctgctgattg tcgatgaaga acaccgcttc ggggtgcgtc ataaagagcg
    19681 tattaaagcg atgcgcgcga acgtggatat tctgacgctt actgcaacgc cgatcccacg
    19741 tacgctgaat atggcaatga gcggaatgcg tgacctgtcg attatcgcca cgccgcccgc
    19801 ccgtcgtctg gcagttaaaa cctttgtccg tgagtatgac agcctggtgg tccgggaggc
    19861 gatcctgcgt gaaattttgc gcggaggaca ggtttattat ctctacaatg atgtggaaaa
    19921 cattcagaaa gccgccgaac ggctggcaga actggtgcca gaagcgcgga tcgccatcgg
    19981 tcacgggcag atgcgcgagc gcgaactgga acgggtgatg aatgatttcc atcatcaacg
    20041 tttcaacgtg ctggtttgta ccaccattat tgaaaccggg atcgacatcc cgacagccaa
    20101 caccattatc attgaacgtg cggatcactt cggtctggcg cagctgcacc agttacgcgg
    20161 tcgcgtcgga cgttcgcatc atcaggcata tgcatggttg ctgacgccgc atccaaaagc
    20221 gatgactacc gatgcacaaa aacgtcttga agcgattgcc tcgctggaag atctcggggc
    20281 aggttttgcg ctggcaacgc acgatctgga gattcgcggc gcgggtgaac tgcttggcga
    20341 agaacaaagc ggctcaatgg aaaccatcgg tttctcgctg tatatggagt tgctggaaaa
    20401 cgccgtcgat gcactgaaag ccggacgcga gccgtcgctg gaagatctca ccagccagca
    20461 aacagaagtc gagctgcgga tgccgtcgct attgccagat gatttcattc ctgacgtgaa
    20521 tacgcgtttg tcgttctata aacgtattgc cagcgccaaa acggaaaacg aactggaaga
    20581 gatcaaagtc gagcttatcg atcgcttcgg cctgctgccg gatccggcgc gtatcctgct
    20641 ggatgttgcc cgtctgcgcc agcaagcgca gaaactgggg atcaggaagc tggaaggtaa
    20701 tgagaaaggc ggggtgatcg aatttgccga gaagaatcac gttaatccgg cctggttgat
    20761 tggtttgctg caaaaacagc cgcagcatta ccgccttgat ggtccgacgc gcctgaaatt
    20821 tattcaggat ttgagtgagc ggaaaacgcg tatcgaatgg gtacgccagt ttatgcgtga
    20881 actggaagag aacgcgatcg cttaatggta aatcgggggc gtttctgcgc ccccatctgc
    20941 aacatttaca aattctttgc acttccctgc actatccgac actgtcacca tccataattc
    21001 aggcttatct gtttattaca ataaccttat atttattatg gatttttggt gatgatcaaa
    21061 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    21121 ccggcaaaag ccaacacctg gccgctgccg ccagcgggca gtcgtctggt tggcgaaaac
    21181 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    21241 gtcggctttc tcgctctgtt acaggctaac cccggcgttg atccttacgt accgcgcgcg
    21301 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    21361 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    21421 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    21481 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    21541 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    21601 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    21661 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    21721 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    21781 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    21841 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    21901 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    21961 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    22021 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    22081 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    22141 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    22201 ccagcgatcc cagatttgtc cccgcgttag cactgattgt accgactttt tgttggcctt
    22261 ctggcgttga ctgaacttcg acagccgcaa agctggcaaa tgacatggag cttaaaatcg
    22321 ccgcagcgat gagggttttt acgtttttca taatagtggc cttatgcaga tgaatgacgt
    22381 cgcaagcaat tacttaacga tcgataacta aatgatagat gtgatctgga tcacatacaa
    22441 gatatttttt ataacaatca ttaattaatt taaaaatctt taaatttcag ttaaatagaa
    22501 a
//
LOCUS       DAZGXZ010000081        22384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000081 DAZGXZ010000000
VERSION     DAZGXZ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.081"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..298
                     /locus_tag="ACHKJF_003229"
     CDS             <1..298
                     /locus_tag="ACHKJF_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HFP0950539.1"
                     /translation="DQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAEL
                     LRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            389..1636
                     /gene="tnaB"
                     /locus_tag="ACHKJF_003230"
     CDS             389..1636
                     /gene="tnaB"
                     /locus_tag="ACHKJF_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP0950540.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFLNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            1768..2943
                     /gene="mdtL"
                     /locus_tag="ACHKJF_003231"
     CDS             1768..2943
                     /gene="mdtL"
                     /locus_tag="ACHKJF_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP0950541.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            2918..3877
                     /gene="yidZ"
                     /locus_tag="ACHKJF_003232"
     CDS             2918..3877
                     /gene="yidZ"
                     /locus_tag="ACHKJF_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP0950542.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWSLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4034..4783
                     /locus_tag="ACHKJF_003233"
     CDS             4034..4783
                     /locus_tag="ACHKJF_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP0950543.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPLHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            4805..5371
                     /gene="yieF"
                     /locus_tag="ACHKJF_003234"
                     /gene_synonym="chrR"
     CDS             4805..5371
                     /gene="yieF"
                     /locus_tag="ACHKJF_003234"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP0950544.1"
                     /translation="MSEKLKVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5425..6762)
                     /gene="adeP"
                     /locus_tag="ACHKJF_003235"
     CDS             complement(5425..6762)
                     /gene="adeP"
                     /locus_tag="ACHKJF_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP0950545.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGNVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            6929..7594
                     /gene="yieH"
                     /locus_tag="ACHKJF_003236"
     CDS             6929..7594
                     /gene="yieH"
                     /locus_tag="ACHKJF_003236"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP0950546.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            7661..8134
                     /locus_tag="ACHKJF_003237"
     CDS             7661..8134
                     /locus_tag="ACHKJF_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFP0950547.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            8183..8770
                     /gene="cbrC"
                     /locus_tag="ACHKJF_003238"
     CDS             8183..8770
                     /gene="cbrC"
                     /locus_tag="ACHKJF_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFP0950548.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(8832..9554)
                     /locus_tag="ACHKJF_003239"
     CDS             complement(8832..9554)
                     /locus_tag="ACHKJF_003239"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0950549.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(9569..10738)
                     /locus_tag="ACHKJF_003240"
     CDS             complement(9569..10738)
                     /locus_tag="ACHKJF_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP0950550.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(10765..12381)
                     /gene="bglH"
                     /locus_tag="ACHKJF_003241"
     CDS             complement(10765..12381)
                     /gene="bglH"
                     /locus_tag="ACHKJF_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP0950551.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(12467..13861)
                     /gene="bglB"
                     /locus_tag="ACHKJF_003242"
     CDS             complement(12467..13861)
                     /gene="bglB"
                     /locus_tag="ACHKJF_003242"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP0950552.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(13880..15757)
                     /gene="bglF"
                     /locus_tag="ACHKJF_003243"
     CDS             complement(13880..15757)
                     /gene="bglF"
                     /locus_tag="ACHKJF_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP0950553.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(15890..16726)
                     /gene="bglG"
                     /locus_tag="ACHKJF_003244"
     CDS             complement(15890..16726)
                     /gene="bglG"
                     /locus_tag="ACHKJF_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP0950554.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(17012..17737)
                     /gene="phoU"
                     /locus_tag="ACHKJF_003245"
     CDS             complement(17012..17737)
                     /gene="phoU"
                     /locus_tag="ACHKJF_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP0950555.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(17752..18525)
                     /gene="pstB"
                     /locus_tag="ACHKJF_003246"
     CDS             complement(17752..18525)
                     /gene="pstB"
                     /locus_tag="ACHKJF_003246"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP0950556.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(18616..19506)
                     /gene="pstA"
                     /locus_tag="ACHKJF_003247"
     CDS             complement(18616..19506)
                     /gene="pstA"
                     /locus_tag="ACHKJF_003247"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP0950557.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(19506..20465)
                     /gene="pstC"
                     /locus_tag="ACHKJF_003248"
     CDS             complement(19506..20465)
                     /gene="pstC"
                     /locus_tag="ACHKJF_003248"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP0950558.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(20552..21592)
                     /gene="pstS"
                     /locus_tag="ACHKJF_003249"
     CDS             complement(20552..21592)
                     /gene="pstS"
                     /locus_tag="ACHKJF_003249"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP0950559.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(21840..>22384)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKJF_003250"
     CDS             complement(21840..>22384)
                     /gene="lpfD-O113"
                     /locus_tag="ACHKJF_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFP0950560.1"
                     /translation="ETKIVTLYGTISRDPPVDYSQPMADVYIRGDITAPQSCEINNLQ
                     PVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTD
                     NSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPASLTG
                     ARPAPGQFTATATITVEIVR"
BASE COUNT         5601 a         5699 c         5299 g         5785 t
ORIGIN      
        1 agaccagttc ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc
       61 ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg
      121 cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga
      181 cttcattatt gaagccttta aacatgtgaa agagaacgcg gcgaatatta aaggattaac
      241 ctttacctac gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt
      301 aatactacag agtggctata aggatgttag ccactctctt accctacatc ctcaataaca
      361 aaaatagcct tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc
      421 attttggggt gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc
      481 tgttgatctt gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt
      541 ttcaatgctt cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc
      601 cagttttaac accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat
      661 taccgttgcc ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat
      721 cattagtgaa acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat
      781 ctgcacagcc attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat
      841 tacctcattg ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt
      901 cttccaggtc gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta
      961 cttcccgtat atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa
     1021 tattcccagc ctgattattt gctatggaaa acgcaaagat aagttaatca aaagcgtggt
     1081 atttggttcg ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa
     1141 tattccgcgc gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt
     1201 gaaatcgttc ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc
     1261 taacttagct gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc
     1321 ggacctgttt aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt
     1381 cctgccacct gcgttgttgt atctgatctt cctgaacggc tttatttacg ggatcggcgg
     1441 tgccgggctg tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc
     1501 tcgcaagaag tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat
     1561 tgtcattctc tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt
     1621 acctaaattt ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg
     1681 aaatcactta tgtccaaatg gcaactcgcc tgatcctcct tcaccacgta tgctttgcgt
     1741 caccttacta tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg
     1801 gttttacttt atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc
     1861 gatctcaatg ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg
     1921 gcagctgcga tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc
     1981 atacccggcg cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc
     2041 acgttatttc ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtagtg
     2101 gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta
     2161 ctcaacggta ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg
     2221 cttaaattcc cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg
     2281 atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa
     2341 ccacgagaaa atagcgagtc gctgcttaat cggtttttcc tcagccgtgt tgttatcacc
     2401 accctcagcg tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa
     2461 atcatgggtt ttgagcgcgg agaatacgcc accattatgg cgttgactgc tggcgtcagc
     2521 atgaccgttt cattctccac gccatttgcg ctgggaattt ttaagccacg tacgttgatg
     2581 atcacctcgc aggtgttatt ccttgcagcg gggatcaccc ttgccgtttc accttcccat
     2641 gcggtttctc tgtttggtat cacgctgatt tgcgccggtt tctcggtagg ttttggcgta
     2701 gcgatgagtc aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta
     2761 ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc
     2821 ggcgcatgga atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg
     2881 attatgttcg tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct
     2941 tgatctcaat ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc
     3001 ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc
     3061 gtggtttgac gacccgctct ttgtgaactc accgctgggc ctgtcgccca caccgctaat
     3121 ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa
     3181 accgcaccac cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat
     3241 gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat
     3301 caaattacgt aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat
     3361 tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt
     3421 agccattgat tatgaagtgc tgtttagtga tgtgccctgc gtctggttac gcaaagatca
     3481 tccggcactg catgaaacgt ggagtctgga cacttttttg cgttatccgc atatcagcat
     3541 ttgctgggaa cagagcgata cctgggcgct ggacaatgtg ttacaggagc tggggcgcga
     3601 acgcacgatt gctatgagcc tgccggaatt cgagcagtca ctgtttatgg cagcgcagcc
     3661 cgacaatctg ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccacca
     3721 actgccgttg gttgcgcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt
     3781 cccttttacc ctgctttggc ataaacggaa cagccataat ccgaagatcg tctggttacg
     3841 ggaaaccatt aaaaatcttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc
     3901 tatttcacaa taaaatgtaa aaattttgta ataaacttgt ctgaatcgaa cttttagccg
     3961 ctttagtctg tccatcattc cagtaaatga ttactcttgt attcataatg gaccattaag
     4021 catggagcga aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat
     4081 ttctgttcgt ctcccctccc agtgtcatca agaagcccga aacattcccc ttcatcgtca
     4141 aagccgtttt ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat
     4201 tggcgaattg ccggaaattg tcaccctgcc gaaaggtaaa ccggttttca gcgataaaaa
     4261 tttgccttcg ttttctattt cctatgccgg gaatatggtt ggcgtggcgt taacaaccga
     4321 aggtgaatgt ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca
     4381 cgcgcccgat aaccacacct tttcaagcaa tgaatcgcta tggatcagta aacaaaacga
     4441 tcctaacgaa gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg
     4501 tgatgttttg aatgacgatc cgcgcgatct gcagctgctg cccattgccg gacgcctgaa
     4561 atgtgctcat gtaaatcatg tagaagcgtt atgcgacgcg gaagacgtgc tggtgtggtc
     4621 cgtggcggtc acgcccacga ttgaaaagct cagtgtctgg gagttagatg gcaaacacgg
     4681 ctggaaaagc ctgccggata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg
     4741 ttttgcccag ctctctaccg tgaaggcttt ttcgccaaat tgatagacaa ccacaggagt
     4801 catcatgtct gaaaaattga aggtggttac gttactgggg agcctgcgca aaggctcatt
     4861 taatggcatg gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aagtcaatgc
     4921 gttaccttcc attgccgaca ttcccttgta tgacgctgac gtacagcagg aagaaggttt
     4981 tccagcaacg gtagaagctc tggcggaaca gatccgtcag gctgacggtg tggtgattgt
     5041 cacgccggaa tataactact cggttccggg cggtctgaaa aatgccatcg actggctttc
     5101 ccgcctgccg gatcaaccgc tggccggtaa accggtgttg attcagacca gctcaatggg
     5161 cgtgattggc ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc
     5221 aatggtgatg aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatccgca
     5281 aaccggagaa gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt
     5341 tggtgagttt attcagcgag ttaagatcta aataaaaaac ccgccagcaa tcatgcatgg
     5401 cgggttttta acgcgctatc gattttagtg agcgtcgata aacacaatct tcaggataaa
     5461 cagcagcgca acgatgatta cgcacgggct tagatcacgc agacgtccgg taccgatttt
     5521 catcacgcag taggagataa agcccagcgc gataccttcg gtaatcgaga agctgaacgg
     5581 catcatcacg gcggtaataa acgccggaac agattcagta agatcctgcc agttcacgcg
     5641 tgccagactg gaagtcatca gcacgccaac gtaaatcagc gcaccagctg cagcgtagcc
     5701 tggcaccatc cccgccagcg gcgacagaaa gataaccagc aggaacagca gaccaacaac
     5761 cactgccgtc agaccggtac gaccgccaac cgatacgccg gaagaggact caatataagc
     5821 cgtaacggaa gaagtaccga taaacgaacc ggtcacggaa gagatactgt cgacatacag
     5881 cgcctgcttc atgcgcggga atttaccctt ctcatccgcc aggcctgctt tatcggtcac
     5941 gccaatcagc gtaccggagg agtcaaacag gttgaccagc atgaaagaga atatcacccc
     6001 tgccagcccg aggttaaacg acccggctaa atctacatgg cccacgacag tcattacgct
     6061 cggcggcgca gaaacgatgc cattgtagtg gacattaccc agcatccagc ccagcagcgt
     6121 tgtcaccacg atggaaacca gcaccgccgc gtgaatgttg cgcgaggcca gaatagcgat
     6181 gatgaagaag ccgaggatcc ccagcagcac gctgtgagaa gtcagattac cgatgctgac
     6241 cagcgtttcc gggttagcga caatcacacc tgcattcttc agtcccatca taccaatgaa
     6301 cagaccgata ccgctggtga tgcccacacg cagactcacc ggaatgttgg caatcatcca
     6361 gtagcgaacg cggaaaatcg tcagtaacag cagacctatc gcgccccaga agattgcgcc
     6421 catcccgact tgccacggca agcccatcgc ctgcacaacg acaaaagcga agaatgcatt
     6481 caggcccatt gccggtgcca gtgcaactgg caggttagca aacagtccca tcataatact
     6541 gccgaatgca gcaatcagac aggtagtgac gaagacggcg ctggtatcca tgccagcaac
     6601 gccaagaatt tgcgggttaa caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc
     6661 ggcgatcact tcggtccgtg ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag
     6721 catcccctgg ccagaagcct gggttgtgtg ttgttgactc attatctatt tccgaacaag
     6781 gagggaaaat ccgtcgctat cgtataccaa aatgagacaa tatggacggc tgaagataac
     6841 tttttttcat tgtatttgct tatacggcaa cgattgcgtc atgcgaattg gcatgacgaa
     6901 aacgttaaac taacgataaa ggaaagacat gtcccagata gaagcggtat ttttcgactg
     6961 cgacggtacg ctggtcgaca gtgaagtcat ttgctcccgc gcatatgtaa cgatgtttca
     7021 ggaatttggt attacgctcg atcctgaaga ggtattcaaa cgtttcaaag gtgtaaaact
     7081 gtacgaaatt atcgatattg tttcccttga acatggtgtt acgttagcga aaacagaagc
     7141 tgaacacgtt taccgtgcag aagtcgctcg gctgttcgat tcagaactgg aagccatcga
     7201 aggggctgga gcgctcctgt cagcgatcac tgcgccaatg tgtgtggtat ctaacggccc
     7261 aaataacaaa atgcagcatt ctatgggcaa gctgaatatg ttgcactact tcccggataa
     7321 actgttcagc ggctacgata ttcagcgctg gaagccagac ccggcgttaa tgttccatgc
     7381 ggcaaaagcg atgaatgtaa atgtagaaaa ctgcattctg gttgatgact cagttgccgg
     7441 tgcgcaatct ggtatcgacg caggtatgga agtgttctac ttctgcgccg acccgcacaa
     7501 taagccgatc gttcacccga aagtcaccac ctttacccat ctttcgcagt taccggaact
     7561 gtggaaagcg cgtggttggg atattacggc atagttcttc acactccctt cacttacccc
     7621 gcttaaattg gcgctcaaag gtaagtaaag ggtgtttgat atgtctgttt cacgtcgggt
     7681 aatacatcac ggactttatt ttgcggtttt aggaccgtta attggtgttc tgtttcttgt
     7741 cctctacata ttcttcgcaa aagaaccgct ggttcttttg gtaataatac aggtacttcc
     7801 attgtttctc ttattgtcga ttactacggg agctattcct gcattgttaa ccggcgtgat
     7861 ggtcgcctgt ctgccggaaa agatcgggtc acagaaaaat tatcgttgtc tggctggtgg
     7921 cataggtggc gtcgttatca ccgagatcta ttgtgcagtt attgtacata ttaagggcat
     7981 ggcttcctcg gagttgtttg aaaacattct ttctggtgac agtctcgttg tccgcatcat
     8041 tcctgcattg ctggcaggtg tggtgatgag cagaatcatt acccgtctgc ccggattgga
     8101 tatttcatgt cctgaaacag actctttaag ttaagcggga tactttatct ttgggctact
     8161 caaaagcaga caggatgtta ctatgactca aaatatcagg ccgttacccc aattcaaata
     8221 tcatcccaag ccactggaaa caggcgcatt tgaacaggat aaaaccgtag agtgcgattg
     8281 ctgtgaacaa cagacgtcag tttattactc gggtcccttt tattgcgttg atgaagttga
     8341 acatctctgt ccgtggtgta ttgcggacgg ttctgctgct gaaaagtttg caggtagttt
     8401 tcaggatgat gccagcatag aaggtgttga atttgagtat gatgaagagg acgaatttgc
     8461 cggtattaag aacacatatc ctgatgaaat gctgaaagag ttggttgaac gcacgccagg
     8521 ttatcatgga tggcaacagg aattctggct tgcgcattgt ggcgatttct gtgcttttat
     8581 cggctatgtg ggctggaatg atataaaaga tcgcctcgat gaatttgcca accttgaaga
     8641 agattgtgag aatttcggta ttagaaattc tgatctagct aaatgcctgc aaaagggtgg
     8701 tgattgtcag ggttatctct tccgctgtct ccactgcggc aagctgagac tgtggggtga
     8761 tttttcgtag ttatttaaat aatgagaaca ggccggagcg taattcatac atccggcctt
     8821 atttcttcag cttaaccgag cgctaattct gcggcagcgg ctttatcagc gattaccatc
     8881 agcgaaggat gcagctgcaa cacggaagct ggaacatctt cggtaaccgg accttgcagc
     8941 acatttttca gtgcctgcgc tttacctgcg ccactgacaa ttatcagcaa gtttttcgcc
     9001 gccatgatgc ttttcggccc catcgtcacg tagctgtcag gcactaacga aaagtcgccg
     9061 cccaactcac catgcgccac aatatcaacc atctctccct ggatcgggaa ctccacggtt
     9121 tgctcatgga aatgggtcgt attcggcaaa ttgccacaaa aatgaccatc tgcacctaat
     9181 cccagcacca ccaaatccag gccgccttca cgcgccagtt tctgatcatg ctcgcggtag
     9241 ttatcaatag tgagcttctg gatattctct tctttgatcc ccgcaggggt gaaaaacaga
     9301 ttacgcagat tggtaatcgt tacaccttct ccctctttgc cgcgaaaggg aatttcatcg
     9361 aaattataga aatagcagtt atcgtaccag ggcttacctt taaccagggt ggtgaggtat
     9421 tcatacatgc ctttgggcgt gctaccggcg gtaattgcca ggttaacacg acgcgtcttc
     9481 gacatataac caagcagatg gtgtgccgcg acacggctca tttcctggta atcttcggta
     9541 atgattaatt tcatcttagt gccttatttt atttaaataa tttctgtacg aaggctgcat
     9601 aggcaggccg ccagacatcc atttcatggt tcaggccggg atattcctgg taatcaaact
     9661 taattttttt ctgctcaagc tcagttttca gcccggcgat atccttgccg gttacgacat
     9721 ctttatcacc aacgaccaca gtaaaattac gtagttgctg gttaatagct gccggatcgt
     9781 tcagccgggc cgcgacacct tcatccggta cggttgtcgt ggtaacacca ctgaatgtgg
     9841 ccagccagcc aaagctttcc agatgattca ttccggaaac cagcgcctgg tacccgcctt
     9901 gtgaaagccc tgccagcgcg cgaccatcgg catctttacg gacattaaaa cgcttgctaa
     9961 tcagcgggat aatatcgttc atcagttcgc gatctgccgc tttagcattc agcggataaa
    10021 agactttacg tctttcctga ggcacgaaat cttcgggaat aatgcccttc gcatcggttt
    10081 cggtatctgg gatcaccacc agcatcggtt taattttccc ttcagccagc aggttatcca
    10141 tgatttgcgg gatacgcccc tgatcgatag cggaacgtcc agtatcacca aagccgtgat
    10201 agaaatagag cactggcaaa ggctcgccca tgccggtgta tcctggcggg gtccagacat
    10261 acatctgacg ttcagattgc aatgcgttgg agtggtaagt tatggcgatc aaatcaccat
    10321 gcgcaacaga acgcgtatcc agataactgc ctggcaccag aatcatactg gagttaacct
    10381 ggcgctgagg cttagtcatt gcggtgccag tatcaatgct gcgtacacca tcaacattga
    10441 aaaaatattc gtacagattg cctttcagga caggtgtgcg ccacgaccag actcccgctt
    10501 cgtctttggt catcgggtga atattgtccg gaaccggaac acccaccact acggagacgt
    10561 tttttgcccc aggggcaaag tagcgaaagg tcacgctgtt atcggcattg acctgagtca
    10621 catactgttt aaccggaata gtgggtgccg ggctggctgg catatccgct gctatggccc
    10681 actgagcaga aataccgctg gcaatagcca gcgttaaagc agcaattttt atattcatcg
    10741 taatattcct ttcattaagt tccattacca ccagatttca gcctgggcac cgacagcaaa
    10801 ctggtcgtct ttattgtctt caaaggtgaa gccatccagt tcgttttcca gggctttgat
    10861 atacgtggcg tagaaacgaa tttccggacg cgaggtcaac atactggtat tgactttaaa
    10921 ggtatggaag agcgtggttt tataaccaga ctcattaaat ttattactat tcgcatcttt
    10981 gttttgctgg gtgaaatagc ccagttcaac acctgtctgg ttatattggt cccaaatata
    11041 ggccgggcga acaaccgcac gaatagattc gaaatcagag tgggcgcctg tttcgtagct
    11101 atatatatcg ttaccgaagg agtaaacaat cgcgttagct acaatgaaat gatcgccaat
    11161 ataggcttcg ccctgcgaag tcagacgtac cgctgttccg ccggtgtgat caccataata
    11221 acgaccatta aaggtggtaa atggacttgc gccagcataa cggccaaagt tactggcgat
    11281 agaattattc gcgaccagga aggagaattc gttgaagcca cctttatcaa atttctgcgt
    11341 taaagatgtg ccaaacatcc aggtatcttt ccagtcataa tagccgttat tatcctgatt
    11401 atctttttcc gatgcgcttt cgtttgccgt gacataacga ccactcacca ttaaggtcgc
    11461 tttatcccat aacgggatat ctttatagcg taaatcaata gtattggtat taatctgctg
    11521 tttgttttgc aggctgcgat cgtaatcatc gacatcttcg cgaaccagcg cgatatcaat
    11581 tttacccgga ccgactttcc agttttccag acctacaccc gctgcggcat cagtacgctg
    11641 cgttttccag tccagcatct ggatttcaat tttcggcgca ccgtgtttac ccacccagaa
    11701 atcagcctct ggcgcaaagg gcaggaaacc tttggtggta acgtacatat cggagaactg
    11761 cataaagttc tcgccaccgg cgttatcgcc aaaccagccg gtagagtact gctgaccaac
    11821 gttaccatcc atcatcacaa cggcatcaac ccgtttgccg ttttcgttgt agacacgttg
    11881 ttttaactgc aaatcaaacc agccggagta ttcgttacca aagcggccca gagaaccaat
    11941 cgcccatgat ttaggtgaac catgagaggc ggtcccccaa ccagaacggt aatagccgtt
    12001 gtaactaaag ccaatttcat ctttcacaaa cttactgaaa tctttcagag tcatggaaga
    12061 ataaatcgag cctgtctcac tggcattttt ctcttcattt ttgaccagta cagcatcagg
    12121 ttttgcggca ctactggtcg ggaacggatt ggcggcatac ccttgatcat tcgtacttac
    12181 gctacgattc accgtcgctg gcgtatattt tttcttctct tcatctttat actttttgag
    12241 ttcgctttgc gtttctctta acgctttttc taataactca aggcgatgtt ccaccgttaa
    12301 tgattcagcc aatgattgtg cagaaaaggc taacggtgcc attaataaga tggcagaggt
    12361 aataagattt cgtctaaaca taatatccct ttatggtgca aagaaagaat taacgtatcg
    12421 catccagact gttctgaatg cgacgataat taaggtgctt tattggttat ttttttaatg
    12481 acagcccccg cgttttgatc acttctgcat accatccgaa gcttttctta cgcgttcttg
    12541 tgaggcttcc ttcgccatta tcatcacgat ccacataaat aaagccgtag cgcttagaca
    12601 tttgtgaatg agatgcactg actaaatcaa ttggccccca actggtgtac cccataatat
    12661 ccacaccatc ggcaatcgct tcatttacct gtaccaggtg atcgtttaaa taggcaattc
    12721 gataatcgtc ctgtatcgaa ccatccgctt caacgctgtc ttttgcgcct aatccgttct
    12781 cgacaataaa taacggtttt tgataacgat cccaaagcgt atttaacaga acccgtaatc
    12841 caaccggatc aatttgccac ccccactctg aacttttcag atgcggattg gggatcatat
    12901 tcagtatgtt gccctgcgca tttttattaa tgctttcgtc gtgggaaaca caaccagtca
    12961 tgtaataact aaaagagatg aaatctacgg tatgttttaa atcttctgcg tcactttcag
    13021 tcatctcaat ggtgatattg tggtcgcgga agaaacgctg catatagccg ggatactgac
    13081 cacgcgcctg aacatcacca aagaacatcc agcgccggtt ctcttccatg gcctccaaca
    13141 tatcctgtgg ctggcaggtg agggggtaaa ccagcccgcc gagaagcata ttgccgattt
    13201 tcgcttcggg gatcaggcta tgacaggctt taactgcccg cgcactggca accagttgat
    13261 gatggatcgc ctgataaact tccgcctcgc cactctcttc tgccagcccc acgcccgtga
    13321 atggcgcgtg taacgacata ttgatttcat taaacgtcag ccataacgct actttatgtt
    13381 ggtagcgggt aaagaccgtg cgggcgtaat gctcgaagtg atcgatgacc gcccgattag
    13441 cccaaccgcc gtagtttttc accagcccat atggcatttc gtaatgggat aacgttacca
    13501 gcggcttgat ccccgcctgc gccatttcat caaacagccg atcgtaaaac gctaaccccg
    13561 cttcattcgg ttcggcttcg tcgccctgag ggaaaattcg cgcccaggca atggaaatac
    13621 gcagacaggt gaagcccatc tcggcaaata acgcgatatc ttccgggtaa cggtgataaa
    13681 aatcgatggc gacatctttg atattctctt tccccaggat gcgcggttcc atttttccca
    13741 ttacgccatg aggctgtaaa tctgaggtcg tgatcccttt gccatcttcc agccaggcac
    13801 cttccacctg attggcagct gttgcgccac cccaaagaaa tgtttctgga aatgctttca
    13861 taattaactc cttttatcgt tagcgaatga tggataacag cggctcacca gcgcttatct
    13921 gcgccgtgcc gtggggtaat acgtctgtaa aatcatcgct attactgatt aataccggcg
    13981 tcgtcagatc aaatccggcc tcgcgaatag cagggatatc aaaagaaatc agccgatcgc
    14041 ctgtattgac cttgtcaccc acgttgacgt gagcggaaaa gaatttgccg tccagtttta
    14101 cggtgtcgat accgacatga atcaggatct ccacaccatc atctgactca atgccaatag
    14161 cgtgtaatgt ggcgaacaac gaagcaattc gacccgcaac cggagaacgc acttcaccaa
    14221 ccgagggcag aatggcgata cctttaccca acaggccact ggcaaacgtg gtatcagcga
    14281 cgtgaatgag cggcacaatc tctcccgtca tcggtgaaca gataccgccc tgctcaggtg
    14341 gtgtaataac ctctggtgtt ttctcttgcg gggcaccctg cgctggctga cgtttagcgg
    14401 tgatgaaatg aagcatcacc gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa
    14461 taacgctggc ccagacggtg aaatcaattc ccgttgacgg gatggtttgc atgaaggtga
    14521 aaatacttgg caaaccaaag gagtacactt tcgtttgcgc gtagccaatg atggtggccc
    14581 ccaaagcccc actgatacag gcgataacaa aggggtactt acgcggcaga ttaacgccat
    14641 ataccgctgg ttcggtgata ccaaacaggc ccgtcaacgc cgctgatccc gccaccactt
    14701 ttttctgcgc atcgcgttcg cagaggaaga cgccgagcgc cgccccgacc tgcgccataa
    14761 tggcgggcat taacagcggg atcatggtgt cgtagcccag cacggtgaag ttattgatac
    14821 acagcggcac caggccccag tgcagtccga acatgacgaa gatttgccag aagccgccca
    14881 ttaccgcgcc cgcaaatgca ggaaccgcct gataaagcca gagataaccg gcggcaatca
    14941 gttcgcttat ccaggttgat agcggcccca ccagcagaaa ggtgacgggt gtgataacca
    15001 tcagacatag caatggtgtg aagaaatttt tgattgccga cggtaaccac gcattaagtc
    15061 ggcgttccag aatgctgcac aaccaggcag aaaaaataat gggaataacc gatgacgagt
    15121 aattcaacaa tgtgaccgga atacccagga aatccagccc cagctcatcc gctttttgcc
    15181 cgttctcgaa agcagtcaga attaatggat gcactaacgc tccaccaatc accatggcag
    15241 taaatggatt accgccgaag cgtttccccg cggtgtatcc caggattatc gggaagaacc
    15301 aaaacaaggc atcactggcg ctgaataaaa ttaaataagt accactttgt tcggtcgtcc
    15361 actgaaaagt gagcgccaga gccagcatac ctttcaggat cccggttgcc gccatcaaac
    15421 cgatcagagg cgtaaaaata cctgaaataa cataaacaaa gcggtttagc agattacctt
    15481 tctcatcatt ttccggtgcc tgttgcgctt tttcgccaag gcctgccacg ctgttgaccg
    15541 ccaggaagac atcggccaca tggttaccta tgaccacctg aaactggcca ccgctttcca
    15601 ccaccataat aataccgggg gtgtttttca gtacctcagc ttgcgctttg ctttcatcct
    15661 ttaacttaaa acgtaatcgc gttgcgcaat gcatcagact aacaatgtta tctgcgcccc
    15721 cgactcctgc gactattttt ctggctaact ccgtcataac ttgccctcta ccgctttgcg
    15781 gcaaaactcc aaaaaaaacc tgaaaaaaac ggcctgacgt gaatcaagca atttttttca
    15841 ggttttgccc gcttagtgcg gtaacaatcc tttactcagt aataatattt cagtgttctt
    15901 tgcgcacgcg ctctatattt atggctaaaa acataatctc tgcgggtgaa attttacgtt
    15961 gatactgcaa accaataaaa atggcgatcc gttccgcaca ttgccatgct tgcgggtaat
    16021 tttgttttac tgcttgttgt aatgattcat cactatcgtt aattgaagca tgttcaagaa
    16081 tacgccagga taaaaacttc agatgtgtaa ccagtcgctg ataactcaag ctttcttcct
    16141 ggtaattaag gctgaactga aattttatta attgcagcat ttcgcgcatt aactgcgtga
    16201 cacctgcaac atcctccata tttccgctca tttgggcact gaccagatgc atggcaataa
    16261 agcccacttc atctttcggt aactgcacgc ccaaccgttt atcaataatg gttaacgctt
    16321 cttcccctag ctggaactct ttcgggtaaa gccgctggat atcccacagc aacgggttgg
    16381 gcagcaacac gttttgctga aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga
    16441 tataaatact gtcctgtaat tttcccaagc gctcctgcgc taaagagata atacgatcac
    16501 aggttgccat cacctcaaga ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt
    16561 catgactgct caaggcatac tctttttcta ttccacttga gttaattctt tcgccagcgc
    16621 gtttttgaaa gccaattcct cgccccatga cgactttttc ccgctgttga tcatcaataa
    16681 ccaccacaac attattgttg agaattttgg tgatttgcat gttcatagca aggacctttt
    16741 ttataaacaa aaaaacccga cttcaccagt attctctggt tatgtcaggt tttgcctgcg
    16801 aatgcagtaa caatccagtc atttattaat ggtttttata acgaacatcc aggttctgaa
    16861 attaatttaa ttgcgtgcag agaaaatagc aatgcgctat tgataaaaat atgaccatgc
    16921 tcgcagttat taactttgtg taattttagg aatttataaa gttatatata acaaatccca
    16981 ataattaagt tattgggatt tgtctggtga attatttgtc gctatctttt cccgccagca
    17041 gtttatccag ctcatcaccg cctacatgac ggaaatcctg ccccttcacg tagtagaaga
    17101 taaactcaca aatattctgg cagcggtcgc caatacgttc gatagaacgc gcgcagaaca
    17161 gcgcagtaag tacgctcgga atggtacgcg aatcttccat catgtaggtc atcagttgac
    17221 gaacaatacc ttcgtattcc tgatcgactt ttttatcttc acgataaata cgtaccgctt
    17281 cgtcaatgtc catccgcgcg aacgcgtcca gcacgtcgtg cagcatctgg atggtatgac
    17341 ggcccagcga ctccagactt accagcaacg gctgatgctg ctgggagaat ttctccagcg
    17401 cagtacggca gattttgtcc gccacgtcgc caatacgctc cagctcggca atggttttac
    17461 tgatcaccat aaccagtcgc aggtcgctcg ccgtcggctg acgtttggcg ataatgcgca
    17521 cgcaggcttc atcgatcgcc acttccatca tgttgacgtt cttgtcgcct tcgatcacgc
    17581 gcttcgccag atcgctgtcc tggttatgca tcgcggtgat tgcatcagaa agctgctgct
    17641 ccaccatgcc gcccatggtc atcacctgcg tgcggatact ttccagttcg gcgttgaact
    17701 ggccggaaat atgtttatta agattgagac tgtccataac gcactcctga atcaaccgta
    17761 acgaccggtg atgtagtctt ctgtttgttt cttcgctggc ttggtgaaca gatcgtccgt
    17821 gttgctgaac tcaatcaatt cgcccaggta cataaacgcc gtgtggtcgg aacaacgcgc
    17881 agcctgctgc atgttgtggg tgacgatcac cacggtgtaa tcctgcttca gttcggtgat
    17941 cagctcttca atacgcccgg tagagatagg gtcgagcgcc gaacacggtt cgtcgagcag
    18001 cagcacttcc gggcgaatgg cgataccacg cgcaatacac agacgctgtt gctgaccacc
    18061 agagagagag taaccgctct ggtgcaattt atctttggtt tcgttccaca atgcggcttt
    18121 ggtcaatgcc cactgcacgc gctcgtccat gtcggcacgg gagagcttct caaacagacg
    18181 aacgccaaaa gcgatgttgt cgtagatgga catcggaaac ggcgtcggtt tctggaacac
    18241 catgcccact ttcgcacgca gcagggcgat atcctgagag ttggtcagga tgttgtcgcc
    18301 atcaagcaga atttcacctt ccgcacgctg ctccgggtac agttcaaaca ttttgttgaa
    18361 ggtacgcagc agcgtcgatt taccgcagcc ggacggcccg ataaacgccg ttacctggtt
    18421 tttagcgata tccaggttga tgtttttcag ggcatggaat ttgccgtagt agaagttcaa
    18481 attacgaacc tgaattttac tcggggcagt ttcaaccata ctcattgcaa tcttttcctc
    18541 atcgtgcctg atgcacttcg cgaatcaggc ttaccgtagg ccggataagg cgttcacgcc
    18601 gcatccggca aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt
    18661 cagcagcagt acgcacaggg taataatcaa caccccggcc caggccagtt gctgccattc
    18721 tgcaaacggg ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat
    18781 catgtccgtg ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc
    18841 ggcaatacgg gcaatcgcca gcaggatacc ggtcatgatc ccggacaccg acgctttcag
    18901 cgtaatcgcg gagatcattt tccacttcgg tgtacccagc gcataagccg cttcacgcag
    18961 gctgtccggc accagtttca gcatgttctc ggtggtgcgg ataacaatcg gcacctgcaa
    19021 cagcgccagc gcaattacac ccgcccagcc agagaagtgc tccatctgcg ccaccacgat
    19081 ggtgtaaaca aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa
    19141 gcgaatcact tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat
    19201 aatgcccagc ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag
    19261 ggcgttcgcc agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc
    19321 cagcgacata ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa
    19381 caggccgaag gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg
    19441 acgcgcctgc attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat
    19501 agccattagc gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg
    19561 aggacgatga aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc
    19621 agaccggatt ccgcttccgc aaattcgttc gccagcgcag aggtgatact gttgcccggc
    19681 atatacagcg aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg
    19741 gtttcaccga gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg
    19801 aacggaagaa cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac
    19861 tctttcatca tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga
    19921 ataatcatga tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca
    19981 gagaacagcg cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag
    20041 taaacggcga acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga
    20101 atggctgcca gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc
    20161 tcagtcagga acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac
    20221 gaagtcacca acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc
    20281 cactctttgg tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg
    20341 atcagagaga caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc
    20401 agcacgctga aaattatgtc gcccttttta cccggtgggt taaaagcagg cttggttgca
    20461 gccataagtt actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg
    20521 cgtaaaacac cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt
    20581 taatattggt cttccacgca gcgcgaacct gttcaactac gctatccggc aggctggcgt
    20641 aatccaggtc gttcgcctgt ttagccccgg ttttgtaagc ccagtcgaag aacttcagca
    20701 cttctgtgcc ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg
    20761 taataggcca tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc
    20821 agtctgcacc ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat
    20881 cagcggagat cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat
    20941 aaccaattgc acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc
    21001 ccagaccgat cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt
    21061 tcactttcgc caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta
    21121 ctacagcaat gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat
    21181 catcccactt cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca
    21241 gttcgccaga cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg
    21301 ggaactggaa cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac
    21361 caaaatcaac ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt
    21421 agttaacttt attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg
    21481 cagggaaggt tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcgctca
    21541 tcgataaggt cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc
    21601 ctgggaggat tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt
    21661 gacagttata tgtaaggaat atgacatttt tatgacagaa aggaattacc aaataactta
    21721 ctgattttaa atgtgaagat gaaaatccca catgtcatct ttttgtcatg atgcatttaa
    21781 aagtgataaa gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat
    21841 tagcgcacaa tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga
    21901 gctccagtaa ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac
    21961 actgttgatt tacccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta
    22021 tttttgtcgt aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc
    22081 gtattgggtt cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg
    22141 ttttcacact caatagtgac cgttttagta attttatgtg ttgttaccgc acttccaaca
    22201 acagatgaaa aatctgcagc tggtatttct ttaaaatcaa aataaactgg ctgtaaatta
    22261 tttatttcgc aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc
    22321 tgtgagtaat ccaccggggg gtcacggcta attgttccat acaatgtcac tatctttgtt
    22381 tcag
//
LOCUS       DAZGXZ010000082        22227 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000082 DAZGXZ010000000
VERSION     DAZGXZ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.082"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            68..940
                     /gene="ygcG"
                     /locus_tag="ACHKJF_003251"
     CDS             68..940
                     /gene="ygcG"
                     /locus_tag="ACHKJF_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0950561.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1000..2298)
                     /gene="eno"
                     /locus_tag="ACHKJF_003252"
     CDS             complement(1000..2298)
                     /gene="eno"
                     /locus_tag="ACHKJF_003252"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP0950562.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2386..4023)
                     /gene="pyrG"
                     /locus_tag="ACHKJF_003253"
     CDS             complement(2386..4023)
                     /gene="pyrG"
                     /locus_tag="ACHKJF_003253"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP0950563.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4251..5042)
                     /gene="mazG"
                     /locus_tag="ACHKJF_003254"
     CDS             complement(4251..5042)
                     /gene="mazG"
                     /locus_tag="ACHKJF_003254"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP0950564.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5113..5448)
                     /gene="mazF"
                     /locus_tag="ACHKJF_003255"
     CDS             complement(5113..5448)
                     /gene="mazF"
                     /locus_tag="ACHKJF_003255"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP0950565.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5448..5696)
                     /gene="mazE"
                     /locus_tag="ACHKJF_003256"
     CDS             complement(5448..5696)
                     /gene="mazE"
                     /locus_tag="ACHKJF_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP0950566.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5774..8008)
                     /gene="relA"
                     /locus_tag="ACHKJF_003257"
     CDS             complement(5774..8008)
                     /gene="relA"
                     /locus_tag="ACHKJF_003257"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP0950567.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8056..9357)
                     /gene="rlmD"
                     /locus_tag="ACHKJF_003258"
     CDS             complement(8056..9357)
                     /gene="rlmD"
                     /locus_tag="ACHKJF_003258"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP0950568.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9414..12170
                     /gene="barA"
                     /locus_tag="ACHKJF_003259"
     CDS             9414..12170
                     /gene="barA"
                     /locus_tag="ACHKJF_003259"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP0950569.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSVLPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12402..13742)
                     /gene="gudD"
                     /locus_tag="ACHKJF_003260"
     CDS             complement(12402..13742)
                     /gene="gudD"
                     /locus_tag="ACHKJF_003260"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP0950570.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLSDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13763..15103)
                     /gene="gudX"
                     /locus_tag="ACHKJF_003261"
     CDS             complement(13763..15103)
                     /gene="gudX"
                     /locus_tag="ACHKJF_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP0950571.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15105..16457)
                     /gene="gudP"
                     /locus_tag="ACHKJF_003262"
     CDS             complement(15105..16457)
                     /gene="gudP"
                     /locus_tag="ACHKJF_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP0950572.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16892..17341)
                     /gene="yqcA"
                     /locus_tag="ACHKJF_003263"
     CDS             complement(16892..17341)
                     /gene="yqcA"
                     /locus_tag="ACHKJF_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP0950573.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17359..18141)
                     /gene="truC"
                     /locus_tag="ACHKJF_003264"
     CDS             complement(17359..18141)
                     /gene="truC"
                     /locus_tag="ACHKJF_003264"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP0950574.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18141..18470)
                     /gene="yqcC"
                     /locus_tag="ACHKJF_003265"
     CDS             complement(18141..18470)
                     /gene="yqcC"
                     /locus_tag="ACHKJF_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP0950575.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19092..19637)
                     /gene="syd"
                     /locus_tag="ACHKJF_003266"
     CDS             complement(19092..19637)
                     /gene="syd"
                     /locus_tag="ACHKJF_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP0950576.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19705..20553
                     /gene="queF"
                     /locus_tag="ACHKJF_003267"
     CDS             19705..20553
                     /gene="queF"
                     /locus_tag="ACHKJF_003267"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP0950577.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20665..22029
                     /gene="ppnN"
                     /locus_tag="ACHKJF_003268"
     CDS             20665..22029
                     /gene="ppnN"
                     /locus_tag="ACHKJF_003268"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP0950578.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5466 a         5914 c         5259 g         5588 t
ORIGIN      
        1 gataacgaat ctaaaattac cgatgccgcc gtcaatttat ttatccagat ttaatgagaa
       61 atttataatg cgctatttca tactaatgtt cactttcgtg tgttcctttg ttgcagccca
      121 accaactatt gtcccacaat tacagcaaca agttacggat cttacgagta gcttaaactc
      181 acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata cccaagtgca
      241 aattgctgta ttaattgttc ccacaaccaa agacgagaca attgaacaat atgctacaag
      301 agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac tgatcattgt
      361 tgcctggtca gatcgtactg tccgcatcca ggtaggttat gggctggaag aaaaggtaac
      421 cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta aacaacaaaa
      481 atttgctcag ggattagagc tagctataaa cgctttgaat aaccaactca cttcacaaca
      541 ccaatatccg actaatcctt cagaaagtga atcagcgtct tccagtgatc attattactt
      601 tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc atcaaggcag
      661 taatttttgt cgcgcatgta aaagtggagt ctatatttca gcaatctatc ttttagattt
      721 attcctgttc tccgataaaa ttttttccat tgctgtattt tccttctttt ttactttcac
      781 catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatcag gtaaaagtta
      841 tcattcagac aacagcggtt ccggtggagg ctcaagtggc ggcggattta gcggcggtgg
      901 cggttcttct ggaggtggcg gcgcatccgg ccgctggtaa ctcaaccata aaaaatgcca
      961 gcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc ctttgatctc
     1021 tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa tcagctggtt
     1081 gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc ctgcagcagt
     1141 accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt gagagataac
     1201 tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg tcagagaacc
     1261 gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac cttctttcag
     1321 gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt cgcccagaac
     1381 tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt cgatagaaac
     1441 gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag aggtgaacgc
     1501 tttgttgcct tcgccagcca gaacgtattt accgtctttg tagaattcag aagctgcgca
     1561 gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa cagcttcagc
     1621 gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac cttcgtcacc
     1681 aactgcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga aaacttcaga
     1741 acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca tgaattcctg
     1801 gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca tcggaaccgg
     1861 catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca gcggcatacc
     1921 tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg cgttcgcgcc
     1981 gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg
     2041 atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc
     2101 tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc
     2161 acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac caccctccag
     2221 atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac gaccgatgat
     2281 ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa actccagaca
     2341 aacaacgcgt acccagggta cgcgttgccg ttctaacttt tttacttact tcgcctgacg
     2401 tttctggaac tcgctggcgg ctttcacaaa gcctgcaaac agcgggtgac catcacgtgg
     2461 agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat tcggaacttc
     2521 gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac ctgcatcttc
     2581 aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct caacaattgt
     2641 cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact gctgtgcgcc
     2701 gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt tgccgttttc
     2761 atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca caaattccgt
     2821 agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat
     2881 acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat
     2941 catgccttct acgccacgat agccgaaacc gccaggtacg aggattgcgt ccagaccttt
     3001 aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga tgttgacgct
     3061 gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat aagcatccgg
     3121 cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt tcgcttcttc
     3181 gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc tgaatcgttt
     3241 acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat agatggaatc
     3301 gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg caatcttcgc
     3361 acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag gctggatacc
     3421 gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt caccagacgc
     3481 tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc caatttcaac
     3541 agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta ctgtaccgcc
     3601 gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct ctttgattgc
     3661 gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt
     3721 acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc tcattttggt
     3781 acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt cttcagtaac
     3841 gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga tgtacggatc
     3901 cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga gggaggctgc
     3961 ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat agttcgttgt
     4021 catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac gggaaagcag
     4081 tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg ccagcgcact
     4141 attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac gcaaatcaag
     4201 cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg cgtaattccc ttagagatca
     4261 atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc aacacctgtc
     4321 atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg ctcaaatttt
     4381 tcgttcgctt tttgcaatgc gatttccgct tttgtcccta aatgacgagc cagattgacc
     4441 gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac aacagcctgc
     4501 cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcgactac cggaccaagc
     4561 gtcgtccagt cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc acgcattaaa
     4621 gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg cgcgcgctct
     4681 tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc agaactatca
     4741 gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca aatatcatta
     4801 aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga
     4861 tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg
     4921 taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt atcccacggg
     4981 cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacggtc gatttgattc
     5041 attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca gaatagaagt
     5101 gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt gtaattcctc
     5161 tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgatac tttttacctg
     5221 atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga acggatatcc
     5281 ttttgattgc gttgtataag gaacacacag acacatacct gttttgttgt tgtacatgaa
     5341 aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg tcgggtcaaa
     5401 atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta ccagacttcc
     5461 ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt gatgtcgttg
     5521 accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat taatttgcca
     5581 tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg cattaacgta
     5641 gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg gatcataacc
     5701 ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat tgtagatacg
     5761 agcaaatttc ggcctaactc ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt
     5821 tgagtttacc cagcacgcgc cccagcactt gcaggttgta aatctcaatg gtcatgtcga
     5881 tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagcacg ttcaccttct
     5941 cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta gccaccacgc
     6001 ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca acaatgcgtt
     6061 ctggcgcatg ggagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac
     6121 cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg cagcagcgcg
     6181 cgatgtggtg catcaggttg ccaacacctt cgaccaccac gcgaccgtta tctttactgc
     6241 ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg tcctgctctt
     6301 cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg agacggatat
     6361 ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa cgcggcagca
     6421 gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg tcaaggattt
     6481 gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg
     6541 aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca cggctggggt
     6601 tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg taggtgaacg
     6661 gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca ctgtggatgt
     6721 ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca cctttcggcg
     6781 taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc atttcgccgg
     6841 aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaagcaatc cggtcttcat
     6901 gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc gcagcaacac
     6961 ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg gttttgccac
     7021 ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg ttagcgacat
     7081 aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc agtgcggcat
     7141 aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac agctcatcga
     7201 aggcgaggtt ctttttctgc attttgcgcc agatactgta gatgtgtttc ggacgaccat
     7261 acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatggccg acaaactctt
     7321 cgatatagtg ttcgcggtcg agacggcgtt catgcagcag ttttgcgatg cgtttgtatt
     7381 cggtcgggtg gagataacgg aagcagtaat cttccagctc ccatttcagt tgcccaatcc
     7441 caagacggtt cgccaacggc gcgtagatat tggtgcactc ttttgccgcc agtacgcgtt
     7501 catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc agtttgatga
     7561 ccacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta tcgacctgtt
     7621 cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca
     7681 cgccgtgaat aaggttaaca accgacttac cgacgctctc acgcagcaca tcttcgctaa
     7741 ctacgttggc atcagccaga gggaacagca gcgccgcccg cagcgtgtca atgtccatac
     7801 ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga ctggcatccg
     7861 gatgcccctg cgtctgttgc agacaatacg cccaggtttc ggctaagcac tcacacgact
     7921 tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca ccagccttat
     7981 tgatatgtgc acttcttacc gcaaccatcg tcctctcctt tagggaccag acctgccgaa
     8041 atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca gatgtcccgt
     8101 gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt ttaataacgc
     8161 ttcgctatcc cgagccagcg ttgcaggatt acaggataca taaactatac gaataggttc
     8221 cagttttata atttgctgca taacacctgc ggcacctgct cgcgcggggt ccagcaacac
     8281 tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa gattttcatg
     8341 ataaaacgtc acattctgta agccattaag acgcgcattc tgctggcctt tttccaccag
     8401 cgccggaaca ccttctacac cgaccacact ggctgcttgt ttcgccaatg gcagtgtaaa
     8461 gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat ccagccattc
     8521 caacgcacgc gctaccattt tttggttcac acccgcattg acctgaataa aatcgcgcgg
     8581 gctaaaagtt aagcgcaacc cgtttgagtc ataccagggc atctcaccag agacggtttc
     8641 gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg aaaagcgttc
     8701 cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc
     8761 gctggttgcc tgtaccagtt caacatgacc aagatggcgc ataacttgta agctgcccag
     8821 gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg ggcattgttt
     8881 aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt
     8941 cggtaagtag ttcagactta aacgcgcgcg gcggcgatag ccccagggaa catcggcgat
     9001 cacttcagag acatcgtgtt tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg
     9061 taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat gaggacagcg
     9121 tggtgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc gggcatactg
     9181 ttttttatct tcagtaacag caacttccgc gttttcctgc ggcaataatc cggggataaa
     9241 taacgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga
     9301 aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg
     9361 cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac tccatgacca
     9421 actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc cttattggtt
     9481 tattgctgag tatctttttc gtcgtgcatc gctataacga cttgcagcgt caactggaag
     9541 atgccggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc atgagcctgc
     9601 aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat tccgatattg
     9661 ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc aattttcatc
     9721 ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag ctcactgtca
     9781 ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt tactcccccg
     9841 acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat attgcgcttg
     9901 agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt tccagcgtga
     9961 tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg cgcgatgtaa
    10021 ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca
    10081 gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt atcaactcga
    10141 tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac caggcgacgt
    10201 ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagtta gatctggcga
    10261 aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat atgtcacacg
    10321 agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg aaaacagaat
    10381 taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat aatttgctgg
    10441 caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg attctggaaa
    10501 gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg gcacattctt
    10561 ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct gataacgtga
    10621 tcggcgaccc actgcgatta cagcaaatca tcactaacct ggtggggaat gcaattaaat
    10681 tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt aataccaaag
    10741 tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc gatcaatcgc
    10801 gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat ggtggcaccg
    10861 gtctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat atttcgttcc
    10921 atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat ctgaacccga
    10981 acattattat cgaagggcca tccacccagt gcctcgccgg taagcgcctg gcctatgtcg
    11041 aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg ccgctggaag
    11101 tggtttatag cccaacgttc tccgtgctgc ctcccgcgca ttacgacatg atgttgttag
    11161 gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg
    11221 tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc aatgcagaaa
    11281 aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct acgcgcctgt
    11341 tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg
    11401 aaagcaagct ggcaatgaca gtcatggcag ttgatgacaa ccccgctaac ctgaaactta
    11461 tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc gggcatcagg
    11521 cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt caaatgcctg
    11581 acatggatgg cattcgggcc tgcgagctca tccaccagct cccccatcag caacaaacgc
    11641 cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg cttggcgcag
    11701 ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat ttgttgttgc
    11761 gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc aacgaaattg
    11821 tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca gcaggaaaaa
    11881 ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca
    11941 aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg attcataaac
    12001 tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg
    12061 aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg ctggaactgt
    12121 tggacgagat ggataatgta gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt
    12181 gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt
    12241 ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa attcaatgaa
    12301 ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt tagcatcagt
    12361 ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac catgcacggg
    12421 cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc atcgtcacgc
    12481 gcgccaagcc cgtgtttctg atacagctca tgggctttca tcacttgatc catatcgatt
    12541 tctacaccca gccccggttt ttctggcacc tgtaccagcc cgcctttgat ctcaaacggt
    12601 tctttggtca ggcgctgatt accttcctgc cagatccagt gcgtatcaat agcagtaatt
    12661 ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg
    12721 tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac acgtaccgaa
    12781 ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg cagggagagc
    12841 gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg caggccagtt
    12901 gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga
    12961 tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc ttcgttcagc
    13021 gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc
    13081 acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg
    13141 aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct
    13201 tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcagc
    13261 ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac ttcgctacgc
    13321 tgttgaccat cgctcagcag ggatgccacg tttaccccca gatgctgccc cagcagatcc
    13381 agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc
    13441 aaaccgcgcc caccagcatc acgatcggca aaagtattac gcaccagcgt cagaacgttt
    13501 ttgtattcac ccagcgtttt acctaccacc agcggaatcg catcttccag cgttttacgg
    13561 attttctcgc cgccgggaat ttcccctacg ccagtgtgac cagaattatc tttgataatc
    13621 acaatattac gcgtaaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca
    13681 tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc
    13741 atatttatgt cctttttcag aatcaatgac ggccgaaaac gggacgttta cggtcaaagg
    13801 tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg acaggcagac
    13861 gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg
    13921 gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg gtcaggcgac
    13981 aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg
    14041 cgcccacatg ggtaaacatc gccagagaga tatcgaaatg gttattagaa tggcagcccc
    14101 aggtcagccc ccagtcgtcg caaagctgcg ccacacggac tgcaccggaa agcgtccaga
    14161 agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatga cccatttcgc
    14221 gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgc cgaaactccg
    14281 ccataacttc acgaccggag aaaccctgtt ctgcgccgca tggatcttcg gcataggtaa
    14341 gaacatcatt cagccctttg catagagaaa tggcttcatc aagtagccag gcaccgttgg
    14401 gatcaacggt aatccgcgca tcagggaagc gtttcttcaa tgcacgaaca gtgtcgattt
    14461 cttgctcgcc aggtaacacc ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct
    14521 gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga tggcgcaact
    14581 gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt ttggtccgat
    14641 caccgatata aaacagataa ccgaggacgg taatagtctc gcgttgcttg cctggcccca
    14701 acagttcgca gaccggaaca ttcagcgcct tacctagcag gtcaagcaag gcggcttcca
    14761 gcgccgccac ggcattaacg cgcaattcaa aagtccaggc acctttgccg aaggtatcaa
    14821 aatcggctgc ctgattacct ttatgcacct gctgaaccac tttattcagg cgcgcaactt
    14881 cctggcccag aaccatcgga atagcatcga ccagtgtttg ataaatcacc tctccgcccg
    14941 gcgcttcacc aatgccggta tgcccggcgt tatcggtgag taccacaata ttgcgggtga
    15001 aatatgcgtt atgtgcccca ccaatattaa gcaacatgct atcgtgcccg gcgaccggaa
    15061 tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca
    15121 ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt
    15181 aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt gccaacgata
    15241 taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca
    15301 ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc
    15361 ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg
    15421 acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta
    15481 cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat cccacccagc
    15541 acaccgccga taaaaccgca aaccgccgga acggaagcca caaagcccgc tttcagaatc
    15601 gacatcccac gcgcctgcac cagataaacc ggaaaccagg taatgaaaaa gtaagtcagg
    15661 gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagttgt
    15721 ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg ctgatccata
    15781 ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac ccccggatgt
    15841 tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc cagaccgccc
    15901 ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg
    15961 aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc
    16021 tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca
    16081 agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc actaaagata
    16141 tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg
    16201 cgttttgaac caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc
    16261 catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc
    16321 atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata
    16381 aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc
    16441 tgacttaaag aactcattgt tgcactcctg aaaattcgcg ttagccacgc tcactctgga
    16501 ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt tttatagatc
    16561 gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag
    16621 cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag
    16681 ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa
    16741 aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa
    16801 aatccgcgct atgaagcagt ttttactggc atttgcctga aaatattcga ttcagcaccg
    16861 ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc cactgttcga
    16921 cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc aacagcattt
    16981 cgccaacccg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg ccgccattgc
    17041 agaaatttac ataactgctg tcaccgagcg caatcacgcc ataacgcaga ttcggctgga
    17101 agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga aggtcgccct
    17161 gcccggtcgt ggacgtaacc accagaacat acttatcctg atagggcagc cagtcgctta
    17221 attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt
    17281 cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca atttccgcca
    17341 taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg gcgcactgaa
    17401 ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata acgcctgcat
    17461 ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa
    17521 tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag caccgctgcg
    17581 attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca aatgggcaag
    17641 atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca ggccgtagcg
    17701 cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac cgcgataatg
    17761 cgtcactgct ggctgcgagc ctttatcttc gcgggcaaat ttatcagcga ttttgtccag
    17821 ttcttccacc agtggataat ccagaactgc ttcttccatc aaccagccgc gcacaatcgc
    17881 atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc gtccggcctc
    17941 gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat gagcggtaaa
    18001 aacgtgctgg ccaatctgat cacgcacggt ttgcatgacc actactttct cgtcgcgatc
    18061 cagccagctg cggtgaacca gccagccgga gggtttattt accgcaacca gccattcatc
    18121 ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc cagtttttcc
    18181 agctcagcca gaataagcgc gcgttgcgga tggtccgtcg ccagcgccat ttcataatag
    18241 ggtgcaacgg caaaagcgcc cggtaacggc tgtttgttat ctaacaaatc gtgcattcgc
    18301 gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa gaacggttgg
    18361 gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt
    18421 aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat aaagcaacct
    18481 caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac tgtattcaca
    18541 gcgctcccgg ttcgtttcgc agcattccag ctacttttgt tgctccctgc tcatccttga
    18601 caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt cctgctcaca
    18661 ccaccccgat gtgatacttc atcctgaagt gtccctggcc ttcctgaccc accgaatcat
    18721 cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg cgtcatcctc
    18781 ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc
    18841 atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga atcgtctttt
    18901 tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca tatgaaagtt
    18961 aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat ctttttaagt
    19021 attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca caaccagatg
    19081 gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag atttgaggct
    19141 aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga gagattacac
    19201 accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg cggcagtttt
    19261 agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg gaagtcgtct
    19321 tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg catatccccg
    19381 gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt
    19441 tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta tacggcatct
    19501 tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact taacggccag
    19561 cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt
    19621 gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt gtcgtttagt
    19681 ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc acttgcgggc
    19741 ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccagcct actgcaaggc
    19801 gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct gccttttcac
    19861 ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg tttgccgcag
    19921 gtcgctgtcg gtcatgttga acttgattac accagcgtaa atctgattga gtcgaagagt
    19981 tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga tgaggtgcgc
    20041 cagacgctgg agcgcgactt aagcacttgc gcccagggta aggttagcgt ggcgttatat
    20101 cgtcttgatg aactggaagg ccagccgata ggtcatttca atggcacctg cattgatgac
    20161 caggatatca ctatcgataa ctatgaattc actactgact atctggagaa tgccaccagc
    20221 ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa ctgcctgatc
    20281 acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca aattgacaga
    20341 gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca cgaacagtgc
    20401 gtggaacgca tctttaatga cctgttacgc ttctgccagc cagaaaaatt gagcgtttac
    20461 gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa tagcgatttt
    20521 gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg cgtgctggat
    20581 tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag ggaatgacga
    20641 tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg ctccatggat
    20701 atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga cctctatcaa
    20761 ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga taacagcaaa
    20821 gaattgctgt ctcgttttga aaatttcgat attaacgtct tgcgccgtga acgcggcgta
    20881 aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat tattcgcgct
    20941 ttgcaggcca acttgttcgc ggtcctgcgt gacattctct tcgtttacgg gcaaatccat
    21001 aacaccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac taacctggtc
    21061 ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc
    21121 tgctggggcg gtcactcaat taacgaaaat gagtatttgt atgcccgtcg cgtcggaaac
    21181 cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc gatggaagcg
    21241 ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag tcgttttatt
    21301 ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt caacgaattg
    21361 atcatcatgc cggatatcga aaaacgtctg gaagcgtttg tccgtatcgc tcacggcatc
    21421 attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct gggaatttta
    21481 atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc gaaagagagc
    21541 gccgactact tccgcgtact ggacgagttt gtcgtacata cgctgggcga aaacgcgcgc
    21601 cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg
    21661 atgccgctgg tgaaagaaaa tcgccgcgac actggtgacg cttacagctt taactggtca
    21721 atgcgcattg caccagattt gcaaatgccg tttgagccgt ctcacgagaa tatggctaat
    21781 ctgaagcttt acccggatca acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc
    21841 tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc gcgccattga agagtttggt
    21901 ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct gctacagggt
    21961 tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg ctacgaaatc
    22021 tgcacgtaat ctccgctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac
    22081 ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc acgcgacaaa
    22141 tatcatcaca gttaatatgt catacaattt atgttgcaac gcaaacgttt ccctattttc
    22201 ataaaccgta ttttatcgct atgaaaa
//
LOCUS       DAZGXZ010000083        21870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000083 DAZGXZ010000000
VERSION     DAZGXZ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.083"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..164)
                     /locus_tag="ACHKJF_003269"
     CDS             complement(<1..164)
                     /locus_tag="ACHKJF_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0950579.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFA"
     gene            359..538
                     /locus_tag="ACHKJF_003270"
     CDS             359..538
                     /locus_tag="ACHKJF_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0950580.1"
                     /translation="MATKAVNAKSKKLEARVPHAIADAVENLKEDGESTGQFIVSALE
                     GEIKRRQRRKAKESE"
     gene            663..989
                     /locus_tag="ACHKJF_003271"
     CDS             663..989
                     /locus_tag="ACHKJF_003271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950581.1"
                     /translation="MNQKDSRHVLLPLSNSLKSTGKSAHCRYGNELNQTDCIAHQPDS
                     GYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQSHQGERIPAHVLAY
                     IQSSWL"
     gene            complement(1039..1227)
                     /locus_tag="ACHKJF_003272"
     CDS             complement(1039..1227)
                     /locus_tag="ACHKJF_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0950582.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(1602..2831)
                     /locus_tag="ACHKJF_003273"
     CDS             complement(1602..2831)
                     /locus_tag="ACHKJF_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0950583.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRTWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLEYYVKENRVDYIALKRRLNNHVIQQIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSNK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEAKQEASVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            2819..3001
                     /locus_tag="ACHKJF_003274"
     CDS             2819..3001
                     /locus_tag="ACHKJF_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950584.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRCTKGYNVKQAGTRKITNLLIIK
                     NIMHSLKHYETAKNTK"
     gene            3008..3913
                     /gene="tqsA"
                     /locus_tag="ACHKJF_003275"
                     /pseudo
     CDS             3008..3913
                     /gene="tqsA"
                     /locus_tag="ACHKJF_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(3938..5326)
                     /gene="pntB"
                     /locus_tag="ACHKJF_003276"
     CDS             complement(3938..5326)
                     /gene="pntB"
                     /locus_tag="ACHKJF_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP0950585.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5337..6869)
                     /gene="pntA"
                     /locus_tag="ACHKJF_003277"
     CDS             complement(5337..6869)
                     /gene="pntA"
                     /locus_tag="ACHKJF_003277"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP0950586.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7393..8337
                     /gene="ydgH"
                     /locus_tag="ACHKJF_003278"
     CDS             7393..8337
                     /gene="ydgH"
                     /locus_tag="ACHKJF_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP0950587.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8523..9905
                     /gene="ydgI"
                     /locus_tag="ACHKJF_003279"
     CDS             8523..9905
                     /gene="ydgI"
                     /locus_tag="ACHKJF_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0950588.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAYAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9942..10664
                     /gene="folM"
                     /locus_tag="ACHKJF_003280"
     CDS             9942..10664
                     /gene="folM"
                     /locus_tag="ACHKJF_003280"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP0950589.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10661..10996)
                     /gene="ydgC"
                     /locus_tag="ACHKJF_003281"
     CDS             complement(10661..10996)
                     /gene="ydgC"
                     /locus_tag="ACHKJF_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP0950590.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            11125..11844
                     /gene="rstA"
                     /locus_tag="ACHKJF_003282"
     CDS             11125..11844
                     /gene="rstA"
                     /locus_tag="ACHKJF_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP0950591.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLSKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11848..13149
                     /gene="rstB"
                     /locus_tag="ACHKJF_003283"
     CDS             11848..13149
                     /gene="rstB"
                     /locus_tag="ACHKJF_003283"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP0950592.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13225..14154
                     /gene="tus"
                     /locus_tag="ACHKJF_003284"
     CDS             13225..14154
                     /gene="tus"
                     /locus_tag="ACHKJF_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP0950593.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGARMPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(14151..15554)
                     /gene="fumC"
                     /locus_tag="ACHKJF_003285"
     CDS             complement(14151..15554)
                     /gene="fumC"
                     /locus_tag="ACHKJF_003285"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP0950594.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAASKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15697..17343)
                     /gene="fumB"
                     /locus_tag="ACHKJF_003286"
     CDS             complement(15697..17343)
                     /gene="fumB"
                     /locus_tag="ACHKJF_003286"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0950595.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17542..18717
                     /gene="manA"
                     /locus_tag="ACHKJF_003287"
     CDS             17542..18717
                     /gene="manA"
                     /locus_tag="ACHKJF_003287"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP0950596.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18818..20326
                     /gene="ydgA"
                     /locus_tag="ACHKJF_003288"
     CDS             18818..20326
                     /gene="ydgA"
                     /locus_tag="ACHKJF_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0950597.1"
                     /translation="MNKSLVAVGVIVSLGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20552..21817)
                     /gene="uidC"
                     /locus_tag="ACHKJF_003289"
     CDS             complement(20552..21817)
                     /gene="uidC"
                     /locus_tag="ACHKJF_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP0950598.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGMGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
BASE COUNT         5406 a         5639 c         5237 g         5588 t
ORIGIN      
        1 gcgaatacag caacacaacc tgacgggcaa cggttacgcg cttcgcgttc cgtccaggcg
       61 gttacgtgga ttatttgaga ttctccggca ctcagtgcca gaaaacgcca cacaaaggcc
      121 gtttgtgtgt gtacaaggtg tggtatatga tttacagcaa ccataacggc tcctagttta
      181 cgttgttggt tagaagccct gcgagtggta acgacacttg cggggctttg tcttttcatc
      241 gattaccttg ataaaggtgt cagccactat attagtaacg ggtgtcagcc acgtcaatag
      301 tgtttttccg ttttcttgtg tgtatactgt cagccaccta ataacgagga aatcagatat
      361 ggcaacaaaa gcagttaatg caaaatccaa aaagcttgag gctcgagtac cacacgcaat
      421 agctgatgct gtagaaaatt taaaggaaga tggcgaaagt acagggcaat ttatcgtttc
      481 ggcacttgaa ggcgagatca aacgccgcca gcgccgcaag gccaaagaat cagaataatc
      541 actatcagcg ccgtggtgtg aggtattacg gcgcattgct atgcaggaca acacaatgac
      601 cgataaagaa ttgaccaaaa cattatcacc ggcacggaaa agacggcgca gaaagataga
      661 acatgaatca gaaagattcg cgccatgtgc ttttgccctt gagcaattcc ttaaagagta
      721 cagggaaaag cgctcattgc aggtatggca acgaactgaa ccagactgat tgcattgccc
      781 accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca cacaaacggc
      841 gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt ttatgcctga
      901 aaaaccccaa ttttgtggtt ttccagtctc accagggcga acgaatcccc gcccacgttc
      961 tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat gtgtggcggc
     1021 tgtgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg ccagaacctc
     1081 ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga attgtccggc
     1141 cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc attctttttc
     1201 acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa agagttattt
     1261 cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag ttggcaaatg
     1321 ttgaggtcgt atggtttaca gaaacaggaa taatcaggat aaaatcattt aaattcatgt
     1381 taatacaaag gcataaaata ttgtttcatg ccttttttct cgccatttaa agagtgattc
     1441 gctagtgtat aaaaaaccag taacacatta aaaatcagcc acttataaat ccgtacactt
     1501 tttcgcctct tgttcgtagt tgttccgcat tgttgctcat tgttgcatgt tgtatctgtt
     1561 cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct tctggctact
     1621 ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc cgtccacata
     1681 tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg ctgcattcct
     1741 ggcatctgat gacctgtaag ctgctccacg acatgcggat caacgcctaa atcgttcagc
     1801 atggttgtaa aggtgcgccg gatgtcatgc agtgaccagt gaggatgatt aagcctcctg
     1861 tgcgctaatc ttccatactg cgatacgctg gcctcctgtt tcgcttcccc cagcaataag
     1921 cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcaggatcgc ttccggtatg
     1981 ggccggaata ttgcgacctt cgttttgctg tgctccttcg gaacggtcca tagcatttcc
     2041 gtaaaatccc actccccgat ctccgatagc ctcagttcta ccgtcctggc tccgaagaca
     2101 atcaggaggc ggattaacgc gacgtagtaa ggggaaaata tttttttgtc cagtgcctgc
     2161 aataattcgc caagttcttt gttacttaag acacgttcgc ttatatccgg ttttttccca
     2221 acgtccgcca cgttcagatc gtccagaacg ttgctgattg catagcgccg cctacggcag
     2281 aacttaagcg cctgtttgca cgcctgtagc acgaatccgg cagtaacagg cgttcgcttt
     2341 gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag cggcatagca
     2401 ccaatctgct gtattacgtg attattaagt cgccttttca gggcgatata atccacgcgg
     2461 ttttccttta cgtaatactc aagccagtag gtgagcgcat cgccaaccgt tacgggcttt
     2521 aacgcttcct gtacggtgta attcatctca tgacgtggat ttttcccctc agccagccat
     2581 gtgcgacact gtgcggcttt ttccctggct gctttcaggc tcagatcagg ataacttccc
     2641 agcttaatgc gttcgggtcg tgtctccctt cccgttccgg ccctgtatgt gaaataccag
     2701 gtcaatttcc ctgatgttaa atatttcacg ctcaggtttc cgccatcact ataaaacgtg
     2761 tttttctccg ctggcttacc atgaagtttc cttagcaagg tatcgctcag tttgttcatt
     2821 gctctgctca cgttttaccc cctctttacg aaaaagtgac tacaccagtg actacaccga
     2881 tcgttgcaca aagggctaca acgtgaaaca agctggaaca aggaaaatca caaatctgtt
     2941 gataatcaaa aacataatgc acagcctgaa acattatgaa acagcaaaaa acactaaatg
     3001 ataaaatgct gttattctta acccgctggt gcaacacatg gtccgctggc gagtgccgcg
     3061 tgtactggcg gtgtcgattt tgatgaccat catcgtgatg gcgatggtgt tgctattagc
     3121 ttatctgggt tccgcgctca acgagttgac gcggacgtta ccgcaatatc gcaactctat
     3181 tatgacgccg ctgcaagctc ttgagccgtt gttgcaacgc gtagggattg acgtctcagt
     3241 tgaccagctg gcgcattaca ttgatccgaa cgcggcgatg acgttgctca ccaacttatt
     3301 gacgcagtta tctaatgcca tgtcatcaat atttttattg ctgctgacgg tgctgtttat
     3361 gctgctcgaa gtgccacaat tgcccggaaa atttcagcaa atgatggcgc gtccggttga
     3421 agggatggcg gcgattcaac gtgcaattga cagtgtttct cattatctgg tgctgaaaac
     3481 agccatcagc atcatcaccg gcctggtcgc ctgggcgatg ctcgccgcac tcgatgttcg
     3541 cttcgctttt gtctggggat tgctggcctt tgcgcttaat tacatcccta atattggttc
     3601 agttctcgcg gcaatccccc ctatcgctca ggtactggtg tttaatggct tctacgaagc
     3661 gttgctggtg ctggcgggat atctgctgat taatctggtc ttcggcaata ttctggagcc
     3721 gcgcatcatg ggacgtgggc tggggctttc cacattggtg gtatttttgt cgttgatttt
     3781 ttggggatgg ttgttaggac cggtgggtat gctgctttcc gtgccgttaa caattattgt
     3841 caaaattgcg cttgaacaaa cagcgggagg tcaaagcatc gccgttctgt taagcgatct
     3901 caataaagag tgacggcctc cgcagaggcc gtcagggtta cagagctttc aggattgcat
     3961 ccacgctggc tttggcgtca ccaaacagca tgtgggtgtt ttccttgaag aacagcgggt
     4021 tttgcacacc agcatagcca gtgttcatcg aacgtttaaa gacaatcacg ttctgcgctt
     4081 tccacacttc cagcacaggc ataccagcaa taggactctt cggatcatcc tgcgccgccg
     4141 ggttaaccgt atcgttagca ccaatcacca gtacggtatc ggtatcagca aagtcatcgt
     4201 tgatctcgtc catttccagc acgatgtcat acggtacttt tgcttcagcc agcaatacgt
     4261 tcatatgtcc aggcaaacgc cccgcaaccg ggtggatacc gaaacgcacg ttgataccac
     4321 gagcgcgcag tttctcggta atttcagcga caggatattg cgcctgcgcg actgccatgc
     4381 cgtaccccgg agtaatgatc actgaatggg agtttttcag cagttccgct gtctcttctg
     4441 cggtgatttc gcggtgctcg cccacttcct gatcatcgcc agtagaagag ccgtcggtgc
     4501 cgaaaccacc cgcaataacg ctgataaagg aacggttcat cgccttacac ataatgtaag
     4561 aaaggatagc gcccgaagaa ccgaccagcg caccggtcac aatcagcagg tcgttgctga
     4621 gcataaagcc cgcagccgca gccgcccagc cggagtacga gttcagcatc gacaccacca
     4681 ctggcatatc tgcaccaccg atggaggcga ctaaatgcca gccgaatacc agcgcaatgg
     4741 cggtcattat cagcaatgcc agcacttgca ggccgacgct atccgtgcga acaaatacaa
     4801 tcagcagcag gaaggaaacg accagagccg ccaggttcat tttgtgacgg tttggcagca
     4861 tcaacggttt agacgaaatc ttgccacaca gtttgccgaa cgccaccacc gaacccgtga
     4921 acgttaccgc cccgatgaag ataccgagga acacttccgt caggtgaata ttgaccagaa
     4981 tcggtgccat tcccgcgtca tgatgcagat aactgttaaa gccaaccagc actgccgcca
     5041 gacccacgaa gctatgcagg atcgccacca gttctggcat ttcggtcatt tcaactttct
     5101 tcgccagacg gataccaatt gccccaccaa tgaccatcgc cagcaagatc cagccaacat
     5161 tacccgtatc cggtccaaaa atggttgcga ttaacgcaat cgccatcccg gcgataccga
     5221 agttgttacc ctggcgagac gtttcatgtt tcgaaagacc ggccagactg aagataaaca
     5281 ggatcgcggc aacaatgtat gcagctgtaa ctaatcctcc agacatatgt taccccttaa
     5341 tttttgcgga acattttcag catgcgctga gtcacggtga agccaccgaa aatattaatg
     5401 ctggctataa gcaccgcgat aaaactaagg aagctaaccc agccgccctg gccaatctgc
     5461 aacagtgctc cgacaacaat aatccctgaa atcgcgttgg tgaccgacat caacggtgta
     5521 tgcagcgcat gcgatacatt ccacaccacg taataaccga caacgcaggc cagcgcgaaa
     5581 acggtgaagt gcccaagaaa ctctttcggc gcaacgcttg ccagccaacc gaaaaggatg
     5641 attgccagcg ccatcaatgc gtatttacgc cacggtgagc aggcacattt ttcctcagtt
     5701 ttcacttccg gtgccgcttt ttgtgccgcc tgcggctgag ctgatacctg aatcggcggt
     5761 gccggccagg taatttcgcc cgcacggatc acggtcacgc cgcgaatcac cacatcatca
     5821 aaatcaacag tgatattgcc gtctttctct ttgcacaaca gtttcagcag attaacgagg
     5881 ttagtaccgt aaagctgtga ggattgcgtc ggcagacggc ccggaagatc ggtataacca
     5941 atcactttga caccattttc cgtagtgaag atttcacccg gcacggtgta ttcacagttg
     6001 ccaccgtttt gggctgccag gtcgacaatc acactgcccg ccttcatgga gtcaaccatt
     6061 tcacgggtaa ttagcttcgg cgctggtttg cctggaataa gcgcggtggt gacaatgata
     6121 tcgacctctt ttgcctgggc ggcaaagagt tccatttccg ctttgatgaa cgcgtccgac
     6181 atcactttgg catagccatc gccgctgccc gcttcctctt taaaatccag ctcgaggaat
     6241 tccgcgccca tactttgaac ttgttctttc acttccgggc gggtgtcgaa tgcacgcaca
     6301 atcgcgccga gactgtttgc tgcgccaatg gcggccagac ctgcaacacc cgcaccaatc
     6361 accatcactt ttgccggtgg cactttcccg gccgcagtaa tttgcccggt aaagaagcgc
     6421 ccaaattcat gtgccgcttc aacaatggcg cgataaccgg cgatgttcgc catcgagctt
     6481 agtgcgtcca gcgattgtgc gcgtgagata cgcggcacag aatccatcgc catcacggtc
     6541 acgttacgtt ccgcaagttt ttgcattaat tccggattct gcgcaggcca gataaaactc
     6601 accagcgttg tcccaggatt cagtaacgca atttcatcat ctaacggcgc attgaccttc
     6661 agaatgatct ctgactgcca gacgctattc ccttctacaa tttcagcgcc cgcttgcaca
     6721 aacgctttat cgtcaaaact tgccagttga cccgcgccgc tctctaccgc gacggtaaaa
     6781 cccagtttca gcagctgttc cactgttttt ggcgttgctg caacacgggt ttcattggtt
     6841 aaccgttctc ttggtatgcc aattcgcatg atattccctt ccatcggttt tattgatgat
     6901 ggtttgcctg tgtcaggagc cacacaagcc gctcatgtac gagctaaatg ttactccgtt
     6961 aaaataaatt agtaacaaac gccctataac gtactgaaaa ttatgcctgt gatctagcgc
     7021 caaaaatcag tatttcggcg tgaactcgca aaatattaac gattcagccg tgatagtggg
     7081 ataaacacct tagaacgccg gataaagact gataattgtc ttcgacggtc gggtaaaacg
     7141 agacatcgcc ccggcacgaa tcactactta acattaaatt aacttatata attcagttgc
     7201 ttcagtagta atgatgctga tacggctgtt ttttaagcat agacggtcat ttgagcagga
     7261 ttaaaattgg cttaaagaat gtgatatgaa aaatgacgca gacagttaca ccgtttaaat
     7321 gcaataatca gccacgtttc tcgttaataa caataccagt acctggtttg cgcaaggcga
     7381 aggattattt ttatgaagct taagaacacc ctcctggcgt cggcactgct ttctgctacg
     7441 gcattctccg ttaacgcagc aacagaactg acaccggagc aagcggcagc ggttaaacct
     7501 tttgaccgtg tagtggttac cggtcgtttt aatgctattg gcgaagcggt gaaagccgtt
     7561 tctcgtcgcg cagataaaga aggtgccgcc tctttttatg ttgtcgacac ttctgatttt
     7621 ggtaacagcg gtaactggcg tgtggtcgct gacctctata aagccgatgc tgaaaaagca
     7681 gaagaaacaa gtaatcgcgt aattaacggt gttgtcgaac tgccgaaaga tcaggctgtt
     7741 ctgattgaac cgtttgacac ggtcaccgtc cagggcttct atcgtagcca gccagaagtc
     7801 aatgatgcca tcaccaaagc ggcaaaagcg aaaggtgcct actctttcta catcgttcgt
     7861 caaatcgatg ccaaccaggg cggcaaccag cgtattactg cattcatcta taaaaaagat
     7921 gctaagaaac gtatcgtcca gagcccggat gtgatcccgg cagattccga agcaggacgt
     7981 gcagctctgg ctgccggtgg cgaagccgcg aagaaagttg agatcccagg tgttgcgact
     8041 accgcatcac caagttctga agtcggtcgt ttctttgaaa cccagtcatc aaaaggcggg
     8101 cgttacaccg tcacgctccc ggatggcact aaagtcgaag aactgaacaa agcgaccgca
     8161 gcgatgatgg tcccgttcga cagcatcaaa ttctctggca actacggcaa catgaccgaa
     8221 gtctcctatc aggttgcgaa acgtgccgcg aagaaaggtg ctaagtatta ccacatcacc
     8281 cgccagtggc aggaacgtgg taataacctg accgtcagcg cagatctgta taaataacta
     8341 ttcagttact cctataaggc ggcttgatgc cgcctttcgc atttttagtc acaaatttcc
     8401 ccgcgaccat tgcatgcaga cacatctctt cgtaaaatcc cgcgcttcag tggcaaactc
     8461 catttttatg cgttttgccc taatatttat tcttcttatc acgttttaat cactggatat
     8521 cgatggaaaa gaaactggga ctgagcgcac tcaccgcgct ggtattaagc tcaatgctgg
     8581 gcgcgggtgt tttcagtctg ccgcaaaata tggcggcagt tgccagcccg gcagcactgc
     8641 tcatcggctg gggtattact ggcgctggca ttttattgct ggcctatgcc atgctgatcc
     8701 tcacgcgcat tcgtcccgaa cttgacggcg gtatctttac ctatgcccgc gaagggtttg
     8761 gcgagctaat cggcttttgt tccgcatggg gatactggct gtgcgcagtc atcgccaacg
     8821 tctcctatct ggtgatcgtt ttttccgcgt taagcttttt tacggacacg ccggaattgc
     8881 gcctgttcgg cgatggtaat acctggcaat cgatcgtcgg tgcatcggct ttattgtgga
     8941 ttgttcactt tttgatcctg cgcggggtgc aaaccgctgc cagcattaac ctggtggcga
     9001 cactggcaaa attgttgccg ctgggtctgt ttgttgtgct ggcgatgatg atgttcaaac
     9061 tggatacctt caagctcgac ttcaccggac ttgcacttgg cgtacccgtt tgggaacagg
     9121 tgaaaaacac catgctgatc accctgtggg ttttcattgg tgtggaaggt gccgtggttg
     9181 tttctgcgcg tgcgcgtaat aaacgtgatg ttggcaaagc gacactgctg gcggttctct
     9241 ccgctctggg cgtttacttg ctggtaacgc tgctttcgct gggtgtggtg gcacgtcctg
     9301 aactggctga aattcgtaac ccgtcaatgg ccggtctgat ggtggaaatg atgggaccat
     9361 ggggcgaaat catcatcgct gccggtttga tcgtttccgt ttgcggcgcg tacttaagct
     9421 ggaccatcat ggcggcagaa gttccgttcc tggctgccac tcataaagca ttcccacgca
     9481 tttttgcgcg ccagaacgca caagctgcgc catccgcctc actgtggttg accaacatct
     9541 gcgtgcaaat ttgtctggta ctcatctggc tgaccggttc cgattacaac acgttgctga
     9601 ccatcgcctc agaaatgatt ctggtgccct atttcctggt cggcgcgttc ttgctgaaaa
     9661 tcgcaacccg tccactgcat aaagcggtag gtgtcggtgc ctgcatttat ggcttatggt
     9721 tattgtacgc ctctggtcca atgcacttgc tgttatccgt tgttctgtat gcgccggggc
     9781 tgctggtttt cctgtatgcg cgcaaaacgc atacacatga taatgtactg aaccgtcagg
     9841 aaatggtgct gattggcatg ctgttaattg cctcagttcc ggcgacctgg atgctggtgg
     9901 gataacgttc atcccatcgt tggatagcaa aggagataac gatgggtaaa gcccagccct
     9961 tgccaatatt aattactggc ggaggtcgtc gcatcggcct cgccctcgcc tggcatttca
    10021 ttaatcaaaa gcaaccggtg attgtcagct atcggacgca ctatccagcc attgatggac
    10081 tgattaaggc aggtgcgcag tgtattcagg ctgatttttc gaccaacgac ggtgtgatgg
    10141 cgtttgccga tgaagtacta aaaagcaccc atggtctgcg tgctattttg cataacgcca
    10201 gtgcgtggat ggcagaaaaa ccaggtacac cactggccga cgtactggct tgcatgatgc
    10261 agatccacgt taatacccca tacctgctca accatgcgct ggaaagatta ctgcgtgggc
    10321 acggacacgc cgccagcgat atcattcact ttaccgatta tgtggtggag cgcggtagcg
    10381 acaaacatgt tgcgtatgct gcaagcaaag cggcactgga taatatgacc cgctcgtttg
    10441 cccgcaagct ggcaccggaa gtgaaagtga attctattgc gccatcgctg atcctgttta
    10501 atgaacatga tgatgccgaa tatcgacaac aggcgctgaa taaatcactg atgaaaaccg
    10561 cgcctggcga gaaagaagtg atcgacctgg tcgattactt acttaccagt tgctttgtca
    10621 ccggacgcag tttcccactt gatggcggtc gtcatctgcg ttaatgcagc tttatccagc
    10681 atataatcaa cacccatgcg cttatccccc aacacgcaac agaaccaaca aaagcggcgg
    10741 gcaagcgcat cattccggtg aaataccaca gcgacaccag gtaaacaaaa tagggaataa
    10801 tcgaccacat actaaaaatg atggttgcgc gtaaggcttc aatgccgcgt tcgctggcaa
    10861 caatataatg cgcgataagc gcaaaggtcg ggaaaagtgg aatcagcccg gcgatataat
    10921 aatttttcgt ttttgctaaa acaccaatca acagcactac cagcgcaccg agcgcggctt
    10981 tgattaccag ccccattttt ttaccttaac acttccataa caagtcatca gtagaatacc
    11041 tgatgaaaac ttgtttagaa acgattgata gtaagtaaaa acagcgcggt gtattgtgac
    11101 gtttttatat ctaccgtgaa tgttatgaac actatcgtat ttgtggaaga tgatgcggaa
    11161 gtcggttcac tgattgccgc gtacctggca aaacatgata tgcaggttac cgtagagccg
    11221 cgcggcgacc aggccgaaga aaccattttg cgagaaaatc cggatttggt gttactcgac
    11281 atcatgctac caggcaagga cggcatgacc atttgtcgtg atttacgcgc aaagtggtct
    11341 ggaccgattg ttcttctaac ctctctcgat agcgatatga accacatcct ggcactggaa
    11401 atgggtgcct gcgactatat tctcaaaacg acgccccctg ctgttttgct agcgcgttta
    11461 cgtttgcatt tgcgtcagaa tgagcaagcc acactgagca aaggtcttca ggaaacgtct
    11521 ctgactccct acaaagccct gcatttcggc acgttgacca tcgatcccat caaccgcgta
    11581 gtcaccctgg ctaacactga aatctcgctc tcgacagctg atttcgaatt attgtgggaa
    11641 ttagctaccc atgccgggca aatcatggac cgcgatgcat tgctgaaaaa tttacgcggc
    11701 gtcagttatg acggactgga tcgtagcgtg gacgtggcta tttcgcggtt aagaaaaaaa
    11761 ctgctcgata acgccgcaga accttatcgc attaaaactg tgcgtaacaa aggctatctt
    11821 tttgcgcctc atgcatggga ataagcgatg aaaaaactgt ttatccagtt ttacctgtta
    11881 ttgtttgtct gcttccttgt gatgtctctg ctggttgggt tggtgtacaa atttaccgcc
    11941 gaacgcgcgg gcaaacagtc gctggatgat ttgatgaaca gttcgctgta tctgatgcgc
    12001 agcgaattgc gtgagatccc cccacacgac tggggtaaga cgctgaaaga gatggattta
    12061 aatctctctt tcgatctgcg tgtcgagcca ttgagtaaat accatcttga tgatatttcc
    12121 atgcaccgac tgcgtggcgg cgaaattgtc gccctggacg atcagtacac gtttttgcag
    12181 cgtatcccgc gcagccacta cgtgctggca gttggtcctg ttccttatct ttattatctc
    12241 catcagatgc gattgctgga tatcgccctg atcgctttta ttgctatttc cctcgccttt
    12301 ccggtgttta tctggatgcg tccgcactgg caggatatgt taaaactgga agcagcggcg
    12361 caacgatttg gcgatgggca tctcaatgag cgtatccact ttgatgaggg ttcgagcttt
    12421 gaacgacttg gtatcgcatt taaccagatg gcggacaata tcaacgcctt gattgccagc
    12481 aaaaaacagc ttattgacgg tatcgctcac gaactgcgaa caccgttagt gcgcctgcgt
    12541 tatcgactgg agatgagcga taacctgagc gccgccgaat cccaggcgtt gaatcgtgat
    12601 atcagtcaac ttgaagcttt aattgaagag ctgctgactt atgcccgact cgatcgccca
    12661 caaaacgagc ttcatcttag cgaaccagac ctgccgttgt ggctgtcaac gcatctggca
    12721 gacattcagg cagtaacgcc cgataaaacg gtacggatta aaacgctcgc gcaaggccat
    12781 tatgcggcgt tggatatgcg cttaatggag cgcgtgctgg ataatttgct caataacgcc
    12841 ctgcggtact gccattcaac ggttgaaacc agcctgctac tgtcggggaa tagagcgaca
    12901 ttaattgttg aggatgatgg cccagggatt gccccagaaa accgcgaaca tatctttgaa
    12961 ccttttgttc gcctcgatcc cagccgggat cgctcaaccg gcggctgcgg gctggggctg
    13021 gcaattgtcc actctatagc actggcaatg ggcggtacgg ttaattgtga caccagcgaa
    13081 ctgggtggtg ctcgcttctc gtttagctgg ccgttatggc ataacatccc gcaatttacc
    13141 tctgcctgac actacgcgca cgatggtcaa gtcaccacga ctgtgctata aaataagtat
    13201 gttgtaacta aagtggttaa tattatggcg cgttacgatc tcgtagaccg actcaacact
    13261 acctttcgcc agatggaaca agagctggct gcatttgccg ctcatcttga gcaacacaag
    13321 ctattggttg cccgcgtgtt ctctttgccg gaggtaaaaa aagaggatga gcataatccg
    13381 cttaatcgta ttgaggtaaa acaacatctc ggcaacgacg cgcagtcgct ggcgttgcgt
    13441 catttccgcc atttatttat tcaacaacag tccgaaaatc gcagcagcaa ggccgctgtc
    13501 cgtctgcctg gcgtgttgtg ttaccaggtc gataaccttt cgcaagcagc gttggtcagt
    13561 catattcagc acatcaataa actcaagacc acgttcgagc atatcgtcac ggttgaatca
    13621 gaactcccca ccgcggcacg ttttgaatgg gtgcatcgtc atttgccggg gctgatcacc
    13681 cttaatgctt accgcacgct caccgttctg cacgaccccg ccactttacg ctttggttgg
    13741 gctaataaac atatcattaa gaatttgcat cgtgatgaag tcctggcaca gctggaaaaa
    13801 agcctgaaat caccacgcag tgtcgcaccg tggacgcgcg aggagtggca aagaaaactg
    13861 gagcgagagt atcaggatat cgctgccctg ccacagaacg cgaagttaaa aatcaaacgt
    13921 ccggtgaagg tgcagccgat tgcccgcgtc tggtacaaag gagatcaaaa acaagtccaa
    13981 cacgcctgcc ctacaccgct gattgcactg attaatcggg ataatggcgc gcgcatgccg
    14041 gacgttggtg agttgttaaa ttacgatgcc gacaatgtgc agcaccgtta taaaccacag
    14101 gcgcagccgc ttcgtttgat cattccacgg ctgcacctgt atgttgcgga ttaacgcccg
    14161 gctttcatac tgccgaccat ctgttctggc cgtacccagc tatcaaactc ggcttcgcta
    14221 agatacccca gcgcaagggc cgcagctttt aaggtcagcc cttctttatg cgcttttttg
    14281 gcgatctccg cggctttgtc ataaccaatg tgggtgttaa gcgcagtcac cagcatcagc
    14341 gattcattga gtaattgatt gattcgctca cgattcggtt caatacccac tgcgcagtgt
    14401 ttgttaaaac tttccatgcc atctgccagc aagcgcaccg attgcaggaa attgtggatc
    14461 accattggac ggaagacgtt cagttcaaag ttaccggaag cgccccccat gttgatcgcc
    14521 acgtcgttcc ccatcacctg acagcagagc atggttaatg cctcacactg tgttggattc
    14581 actttccccg gcatgattga gctgcccggc tcattttccg ggattgagat ttcaccaatt
    14641 ccgcagcgcg ggccagaggc cagccagcgg acatcattgg cgattttcat cagtgacgca
    14701 gccaaccctt tcaacgcgcc gtgcgcctga accagggcat cacaggtcgc aagcgcttca
    14761 aatttgttcg gcgcggtaac aaacggtgca caggtaatga ctgccagttc atctgctacg
    14821 cgacgcgcat actccggatg ggtatttagt ccagtaccca ccgctgtacc gccaagagcc
    14881 agttccgcta cgtgaggcag gctgtattcg atatgtttga gattatgctc gagcatcgct
    14941 acccagccgg aaatctcctg ccctagtgtt agcggcgtgg cgtcctgcaa gtgggttcga
    15001 ccgattttga cgatatcggc aaatgcacgc gatttttcac tcagcgtctg tgtcagggtt
    15061 ttaagctgcg gaatgagttg cttgcgcagc gccagcagcg ccgcaacgtg catcgccgtc
    15121 ggaaagacat cgttggaact ttggcttttg ttcacgtcgt cgttagggtg aactttacgt
    15181 tccatcccgc gcacgccgcc gagtaattca ctggcccggt tagccagcac ttcgttcatg
    15241 ttcatgttac tttgcgtgcc ggagccggtc tgccagatag ccagcgggaa ttcgtcgtca
    15301 tgctgtcctg ccagtacttc atccgccgcc tgccgaatgg cgctcgcttt ctcttcagac
    15361 aacaagccta aatcttcatt aacttttgac gctgcgcgct tggttagcgc cagcgcatga
    15421 atcagtgagg tgggcatttt ctccgtcgaa atgcgaaaat gctccagcga gcgttgagtt
    15481 tgtgcgcccc acagcttatc tgccgggaca tcaatcgccc ccatcgaatc tttttcgctg
    15541 cgtactgtat tcatggcctg ctcctcacct gtttaatttt ttctttctgt tttgctttcg
    15601 ttaagcaact tttagctcac ttattattta ctatttgata acaaatgttt ggtctttcgt
    15661 gccatgtaaa aaaaccgccc cgaagggcgg ctctgtttat ttcacacagc gagtgcactg
    15721 tgtgagttgt atctgctgga agaagtcatt tcctttatca tccacaagga taaacgccgg
    15781 gaaatcttcc acttcaattt tccagatggc ttccattccc agttccggat attcaacaca
    15841 ttccaggctc ttaatacttc cctgcgccaa tacagcggcc ggaccaccga tactgccaag
    15901 gtagaagccg ccgtgttttt tacaggcatc cgtcacctgc tggctgcggt tgcctttcgc
    15961 cagcatgatc atacttccgc cctgcgcttg cagttgatcg acataagaat ccatccgtcc
    16021 ggcggtcgtt gggccaagag aaccggaggc ataaccttcc ggcgttttgg ccggacccgc
    16081 gtagtaaatc ggatgatctt taatgtactg cggcagccct tcaccgttat ccatccgctc
    16141 tttcagtttg gcgtgagcaa tatcacgacc gacgataatc gtgccgttaa gcgataagcg
    16201 tgtagaaacg ggatactgcg acaactgtgc gaggatctct ttcatcggac ggttaaggtc
    16261 aacgcgcacc gcttcgcctt ctcccgcttt acgcagctct tcagggatat atttacctgg
    16321 attatgttcc agtttttcga tccagatccc ctgacggttg atcttcgctt tgatattacg
    16381 gtcagcagag caggaaacac ccataccaac cggacaggat gcgccgtgac gtggcaggcg
    16441 aatcacgcgt atgtcgtgag cgaagtattt accaccaaac tgcgcaccca gaccgagatt
    16501 ttgcgcttcg atcagcaatt ctttttccag ttccacatcg cggaatgcct gaccgtgctc
    16561 attcccttcc gttggcagtt cgtcatagta tttagcggaa gccagtttca ctgttttaag
    16621 gttagtttct gcagaagttc caccaattac gaacgcaata tgatacggag gacaggccgc
    16681 cgtacccagc gtgcgcatct tctcaaccag gtaatttttc agtttccccg gcgttagtaa
    16741 cgctttggtt tcctgataga gatacgtctt gtttgccgaa ccaccacctt tggcgataca
    16801 gaggaatttg tactcgtcgc catcaacggc ataaagatcg atctgcgctg gcagattggt
    16861 gccggtattc acctctttat acatatccag cggcgcgttt tgcgagtagc gcaaattatc
    16921 ttcgatataa gtgttataga caccgcgcgc cagcgccgct tcatcacagc caccggtcca
    16981 tacacgctgc ccttttttac caacaataat cgcagtgccg gtatcctgac aggttggcag
    17041 aacacctttc gccgcgatgt cggagttgcg caggaattgc agcgccacat atttatcatt
    17101 ttcgctggct tccgggtcac gcagaatgtc ggccacctgt tgttggtgag ccggacgcag
    17161 cataaatgac gcatcatgaa atgcctgacg cgccaacaga gttaacgctt cgggtgcgac
    17221 tttcaaaatc tcctgccctt caaattcaga tacgctaacg tgttcgctgg ttagcaggta
    17281 atactcagta tcatcttttt tgagtggaaa aggagcctga taatgaaagg gtttgtttga
    17341 cattgttctc tcacttactg cctggtttgg ttatgctctg ggcgggtgtt ccgttgccct
    17401 gttaaaagcg agtaacaata tcctacacac ttttttaaca aaaactgaga ctagtacgac
    17461 tttttgcggc tccaggttac ttcccgtagg attcttgctt taatagcggg attaatttcc
    17521 acattaaaac agggattgat catgcaaaaa ctcattaact cagtgcaaaa ctatgcctgg
    17581 ggcagcaaaa cggcgttgac tgaactttat ggtatggaaa atccgtccag ccagccgatg
    17641 gccgagctgt ggatgggcgc acatccgaaa agcagttcac gggtgcagaa tgccgccgga
    17701 gatatcgttt cactgcgtga tgtgattgag agtaataaat cgactctgct cggagaggcc
    17761 gttgccaaac gctttggcga actgcctttc ctgttcaaag tattatgcgc agcacagccg
    17821 ctctccattc aggttcatcc aaacaaacgc aattctgaaa tcggttttgc caaagaaaat
    17881 gccgcaggta tcccgatgga tgccgccgag cgtaactata aagatcctaa ccacaagccg
    17941 gagctggttt ttgcgctgac gcctttcctt gcgatgaacg cgtttcgtga gttttccgag
    18001 attgtctccc tactccagcc agtcgcaggt gcacatccgg caattgctca ctttttacaa
    18061 cagcctaatg ccgaacgttt aagcgaactg ttcgccagcc tgttgaatat gcagggtgaa
    18121 gaaaaatccc gcgcgctggc gattttaaaa tcggcgctcg acagtcagca gggtgaaccg
    18181 tggcaaacga ttcgtttaat ttctgaattt tacccggaag acagcggcct gttctccccg
    18241 ctgttactga atgtggtgaa attgaatcct ggcgaagcga tgttcctgtt cgctgaaaca
    18301 ccgcacgctt acctgcaagg cgtggcgctg gaagtgatgg cgaactccga taacgtgctg
    18361 cgtgcgggtc tgacgcctaa gtacattgat attccggaac tggttgccaa tgtgaaattc
    18421 gaagccaaac cggctaacca gttgttgacc cagccggtga aacaaggtgc agaactggac
    18481 ttcccgattc cagtggatga ttttgccttc tcgctgcatg accttagtga taaagaaacc
    18541 accattagcc agcagagtgc cgccattttg ttctgcgtcg aaggcgatgc aacgttgtgg
    18601 aaaggttctc agcagttaca gcttaaaccg ggtgaatcag cgtttattgc cgccaacgaa
    18661 tcaccggtga ctgtcaaagg ccacggccgt ttagcgcgtg tttacaacaa gctgtaagag
    18721 cttactgaaa aaattaacat ctcttgctaa gcttattaaa ggcttataac accttcaggc
    18781 ggccagtccg cctgatttca ttttatggat aatcattatg aataaatcgc tggtagcggt
    18841 aggcgtcatt gtttcgctag gcgtagtctg gacaggcggc gcatggtata caggcaagaa
    18901 gattgaaacc catctcgaag acatggtcgc gcaggcgaac gcgcaactca aactgaccgc
    18961 tcctgaatcc aacctggaag tgagttatca aaactatcat cgcggcgtat tcagcagtca
    19021 gctgcaactg ttggtgaaac ccattgccgg gaaagaaaat ccgtggatta aaagcggtca
    19081 gagcgtcatc ttcaacgaat cggttgatca tggtcccttc ccgcttgccc agcttaaaaa
    19141 actgaacctg atcccgtcga tggcatcaat tcaaaccacg ctggttaata acgaagtaag
    19201 caaaccactg tttgatatgg caaaaggtga aacgcctttt gagattaact cgcgcattgg
    19261 ttacagcggt gattccagtt ccgatatttc gctcaagcca ctgaattacg agcaaaagga
    19321 tgaaaaagtc gcctttagcg gcggcgagtt ccagttaaat gcggacagag acggcaaagc
    19381 tatctccctt tccggggagg cgcaaagtgg tcggatagac gcagttaacg aatacaacca
    19441 gaaagtgcag ttgaccttta ataatctgaa aaccgacggt tccagcacgc tggcaagttt
    19501 tggtgagcgc gtaggaaacc aaaaactgtc actggaaaaa atgaccattt cggtggaagg
    19561 caaagaactg gcactgctgg aaggcatgga gatcagcggt aaatcggatc tggtcaatga
    19621 tggtaaaacg atcaatagcc aactggatta ctcgctaaac agcctgaagg tacagaatca
    19681 ggatctgggc agcggcaagc tgactttaaa agtcggccag attgatggtg aagcctggca
    19741 tcagtttagc cagcaatata acgcgcaaac tcaggcgctg ctggcacagc cagaaattgc
    19801 caacaatccc gaactttatc aggagaaagt gacggaagcc ttctttagcg ccctgccgct
    19861 gatgttgaaa ggcgatccgg tgattactat cgcgccgcta agctggaaaa acagtcaggg
    19921 tgaaagtgcg ctgaatctgt cgctgttcct gaaagatccg gcaacgacta aagaagcgcc
    19981 gcaaacgctg gcgcaggaag tggaccgctc ggttaaatct ctggatgcga aactgaccat
    20041 tccggtggat atggcaactg agtttatgac tcaggtagcg aagctggaag gttatcagga
    20101 agatcaagcg aaaaaactgg cgaaacagca agttgaaggt gcatcagcaa tggggcagat
    20161 gttccgtctg accaccttgc aggacaatac catcaccacc agcctgcaat atgctaacgg
    20221 tcagataacg ttaaatgggc agaaaatgtc gttagaagat tttgttggca tgtttgcgat
    20281 gccagctctt aacgttccgg ctgtaccggc aattccgcag cagtaattca cgaccaaaac
    20341 gccggagaat tttcactccg gcgttttcat ctgaaaacaa accagagatt gcctgatgcg
    20401 ctacgcttat caggcctacc tttacctgca acactttgaa tttatgagtt tttgtaggct
    20461 ggataaggcg tttacgccgc atccggcata aaaaacgcgc actttgtcaa caatctgaaa
    20521 cgccggaggt ttttctctcc ggcgttattt cttacttcag cataaagtca tactgaatac
    20581 tcattttcac atcccagtaa ttcaggcgat cgcccagcgt actgccatcg cgcttttccc
    20641 gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg ctgacccgat
    20701 agttcatacc acctaagata cccatgcctt caatccgcac atctcgttta ctggattcat
    20761 agccgcgagt cacaatcggt gcaaatgtca gtgtaaattg cggcgttaaa cgatagttga
    20821 cgcccaattg ccaggactgc attccttcac ggttacgatc aagacttaat gaaaagggat
    20881 agccaatgtc ggtatccacc acggcaccat tcattgcatg gcgcaaataa ccaccactgc
    20941 caaatacgct tccccacggc gcattccagg tcaattgtcc actaaccaac gccgtttcat
    21001 tgggctggct ggtattacga cttaaacctt cgagctgcgc gtaaaagccg agcaactccc
    21061 ctttcaacgt ataattcggg tcgagcggta tgcttttctc gagaatactt tgtatctgat
    21121 tggaaacatc tttctgtacc gatgccccca gagaatagcg actggtatca tcatgccaac
    21181 tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt tcccgtctgg ctgttataca
    21241 ccccttcatc acgaataccg gttgttgaat cagtccataa aaatactcgt gaacgccaca
    21301 ttccccacag atcaatattt ggtgttaaag cccaataacc taatgctcct tcagaaactg
    21361 tcggtaatgt acgttgcgaa ctgccagcca ttaacggaat acgataaggt aagctaccat
    21421 aactataatc cgtcccgaag cgacctaatt taagcaggct attttcactg ggttttattt
    21481 ttactgcccc cagggcaaag ccaaaacttt tatcaccatc aagataccac cgggtactca
    21541 tatcagcacg agcacctaat ttataaacat aatacgcccc gccttcaacg ccaataatat
    21601 tggaataata accactattg aaatcgagca atcctccctg tacccatgcg taaatatctc
    21661 ttcccgcgcc agcactgggc ttatccgccc tgcgcaattc gtttttcagt ctgatgcgta
    21721 gtcccgcttc atcgtcagcc agttgcgccg cataagcaga ggtaaggcca gaggcagccg
    21781 tcaggcaaat aacggccatg gccactattt ttctcatagt tgcacctttg atgttctgat
    21841 tatttttatt gaatattaat tagtgatatc
//
LOCUS       DAZGXZ010000084        21406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000084 DAZGXZ010000000
VERSION     DAZGXZ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.084"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="ACHKJF_003290"
     CDS             complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="ACHKJF_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP0950599.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLI"
     gene            complement(281..394)
                     /gene="cydX"
                     /locus_tag="ACHKJF_003291"
     CDS             complement(281..394)
                     /gene="cydX"
                     /locus_tag="ACHKJF_003291"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP0950600.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(409..1548)
                     /gene="cydB"
                     /locus_tag="ACHKJF_003292"
     CDS             complement(409..1548)
                     /gene="cydB"
                     /locus_tag="ACHKJF_003292"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0950601.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="ACHKJF_003293"
     CDS             complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="ACHKJF_003293"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP0950602.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(3979..6612)
                     /gene="mngB"
                     /locus_tag="ACHKJF_003294"
     CDS             complement(3979..6612)
                     /gene="mngB"
                     /locus_tag="ACHKJF_003294"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP0950603.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(6630..8546)
                     /gene="mngA"
                     /locus_tag="ACHKJF_003295"
     CDS             complement(6630..8546)
                     /gene="mngA"
                     /locus_tag="ACHKJF_003295"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP0950604.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            8715..9437
                     /gene="mngR"
                     /locus_tag="ACHKJF_003296"
     CDS             8715..9437
                     /gene="mngR"
                     /locus_tag="ACHKJF_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP0950605.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(9541..10410)
                     /gene="sucD"
                     /locus_tag="ACHKJF_003297"
     CDS             complement(9541..10410)
                     /gene="sucD"
                     /locus_tag="ACHKJF_003297"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP0950606.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(10410..11576)
                     /gene="sucC"
                     /locus_tag="ACHKJF_003298"
     CDS             complement(10410..11576)
                     /gene="sucC"
                     /locus_tag="ACHKJF_003298"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP0950607.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(11670..12887)
                     /gene="odhB"
                     /locus_tag="ACHKJF_003299"
     CDS             complement(11670..12887)
                     /gene="odhB"
                     /locus_tag="ACHKJF_003299"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP0950608.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(12902..15703)
                     /gene="sucA"
                     /locus_tag="ACHKJF_003300"
     CDS             complement(12902..15703)
                     /gene="sucA"
                     /locus_tag="ACHKJF_003300"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP0950609.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     VVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(16004..16720)
                     /gene="sdhB"
                     /locus_tag="ACHKJF_003301"
     CDS             complement(16004..16720)
                     /gene="sdhB"
                     /locus_tag="ACHKJF_003301"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP0950610.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(16736..18502)
                     /gene="sdhA"
                     /locus_tag="ACHKJF_003302"
     CDS             complement(16736..18502)
                     /gene="sdhA"
                     /locus_tag="ACHKJF_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP0950611.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(18502..18849)
                     /gene="sdhD"
                     /locus_tag="ACHKJF_003303"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(18502..18849)
                     /gene="sdhD"
                     /locus_tag="ACHKJF_003303"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP0950612.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(18843..19247)
                     /gene="sdhC"
                     /locus_tag="ACHKJF_003304"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(18843..19247)
                     /gene="sdhC"
                     /locus_tag="ACHKJF_003304"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0950613.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            19628..19735
                     /locus_tag="ACHKJF_003305"
     CDS             19628..19735
                     /locus_tag="ACHKJF_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950614.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            19941..21224
                     /gene="gltA"
                     /locus_tag="ACHKJF_003306"
     CDS             19941..21224
                     /gene="gltA"
                     /locus_tag="ACHKJF_003306"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP0950615.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         5282 a         5770 c         5265 g         5089 t
ORIGIN      
        1 aaaatcagcc cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa
       61 agaaccttct gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacatc
      121 agcaataaac catgccagat ttccagttca ctggtcttcg cggcaaaacg agatgggtcc
      181 caaaacatac atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc
      241 ttgtccatta ccgcatacaa agtcgcgata atcttactca tcagatgtct tcttgaccgg
      301 ctttgcctga ttcgacgtgt tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa
      361 gaagcgttcc cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga
      421 gtgggtgtta cgttcaatat cttctttggt gatacgaccg aacattttcc agtaacacca
      481 ggcggtgtag agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag
      541 cgtcagctgg ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga
      601 cggcatcaca aacgggaaca ttgcgatacc ggctgtcagg atgatgcagg ccagcgtcag
      661 ggaggagaac acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag
      721 cggcagaacc acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac
      781 cagccatgcg ccagcttcac gaaccacttc tttattcagt gggttagagg ctgcgtaatg
      841 gtccattgtc gatttcacga cataaccatc gataccgtac atcacccata cgccagccag
      901 tgcgaaacag accagtgtca ccagcgcagc cacctgagcc gttgcacggg tacgcaggtg
      961 cagttcgccc acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac
     1021 gctcaccacg cctgccagca ggccgaacgg gttaagcaac tggaagaagt taccggtgta
     1081 gtacagacgc agatattcat caacgttgaa cggtacgccc tgcaacaggt taccgaacgc
     1141 tacaccaatt accagcggcg gaacgaagct accaatgaag atgccccagt cccacatgtt
     1201 acgccagcgg gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa
     1261 agacgccagc acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat
     1321 cggccaggca gcaaagagtg cgccgcccgc ggtgatcagc caaacctggt taccgtccca
     1381 gtgtggtgca atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg
     1441 ggtgagcatg cccaccccca tgtcgaaacc gtcagtgact gcaaaaccaa tcagcagaac
     1501 gccaaccagc agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct
     1561 gtcttagcgt gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt
     1621 caggctgctt gggccgaggc gtgcaaattt gaacattaag aacaattctg ccaccaggaa
     1681 cagggtatac aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga
     1741 cgagttcgcc acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc
     1801 agccacgaac cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc
     1861 gcgcagaagc cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc
     1921 gatgattgcc agaagcagga agccacacgc caccatgata cggaacgcaa agtacagcgg
     1981 cgctacacgc gggatggagt ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc
     2041 cacgtttggc gtatagcgtt tcagcagcag accgtaaccg aggtctttct tcatgctatt
     2101 gaactggtca cgaaccgcct ggtcggtaga accagaacgc agttgttcga gcagagagta
     2161 cgccttcatc ccgttacgaa tgcgttcttc atgctgcacc atcagctctt tcaggccgat
     2221 aaccggggta tccacggaac gcgttgcaat gatgcccagt gcgtaaggga tctgaatcgc
     2281 aaatttgttc gtctcctctt cctgatcagg aatgccgaac agagtaaagg cagcaggcgc
     2341 aggttgcgtt tcccactcgg cttcaatagc agccagtttg gttttctgca cgtcgcccat
     2401 ttcgtagccg gattcatcac ccagaacaat aacagacaga acagcagcca taccgaagct
     2461 ggcagcgata gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca
     2521 tgcgctgata ccgaggatga acatcgcgcc agtcacataa ccagacgcta cagtgtgaac
     2581 gaatttcacc tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg
     2641 catagtttca aagttgaaat cggacgcgat tgggttttgc atccagccgt tcgcaaccag
     2701 aatccacagt gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg
     2761 aactttaccc agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa
     2821 gaaggccatc agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga
     2881 atagtaagac cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag
     2941 agcgaagttg ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt
     3001 gccggagagg acgtagaccg tttccataat ggccagcagg aacgccatac cgagcgtcag
     3061 tggcacaaaa aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac
     3121 tatatctaac atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta
     3181 gaaagggtca cacgcatgcc ccaatagaaa tttgtttgaa cactttcatc ataccatgtg
     3241 aatgcacaca aaaacggcgt aaagaaaggt tgcaaacatg ttaataaaaa ctcaaattga
     3301 tcccacgtat atattacgcc gcaaaatcct tacaataaac aggtttttgt tgaagcaaat
     3361 ttgcattttt cgacggtgat caatttatag ctaaattacc gcctttcagc caatttgatc
     3421 gagaacaatt tatctctttt tgatgcccat ttccaagact tatacattga taaatatcaa
     3481 ttcctacaaa ttatgttaat catgtttcaa taaggtaact ttagtgacat ttatgtttaa
     3541 aatgtgtgag ttataaggct gaaattagtt tcggttggca attaaagata aaaagagacg
     3601 aagcaaaaac agcacggttg accttaattc tttttttggt cactggctaa tacgacgcag
     3661 agtttcagga ttactaataa aggcataaat ccaccccgct gatattaaca tttaattaac
     3721 ttataattat attataatta actcacccag ggttattatt ttgttaaaaa taaagccaca
     3781 tatacgtggc ttgagagtga agataaacgc aaaattacct gatgcgctac gcttgccagt
     3841 gtctacatca tctctgcaat atattgaatt tgcctgcttt tgtagaccga ataaagcgtt
     3901 catgccgtat ccggcatgaa caaagcgccc tttgtcaaca atctggccgc gcatatgtgg
     3961 cttcggtttg attgctattc aggcaagccg gtaactgaac gtccgtgact ggccgggtaa
     4021 aaaaggcccc gatagatttg aaccttgatt ttcctcggtg gtaatgtgtt catccatcat
     4081 cgtttctgag caagaaatca cctcgcgact gaaagcaaca gtcgcatcac aggttgctga
     4141 ttcagccgga ttaaacagcc gtaaaatcac ttcttgtcgg tcttcagctt tcttaagtgc
     4201 gcttatcagg catcccactg ggggcatttt caacaaacta taactttccg gcacgttgaa
     4261 tccggctttg ttgagcttca tcgcatccca tgggattttg ttgtaacact gtactggagt
     4321 cagccatgct cgcgcctgct gagctacacc agcggcggtt ggcgtaccgg tataactcaa
     4381 taaacttagg cgacaagaaa gcagaccacg tagttgtgag tccgggactg gcattttaat
     4441 tcccgaaggc cgcccaggcc ttaaaaacag atcttctttg cccagtaagc ccacgccacg
     4501 cagcaacgta atggcaaagg ttttcttctc ttcaccgatg acttcaaatt cacgtagccc
     4561 ttcgctaaag acagccatgc cgttacgccc ttcctgtaag gctgcatagt tgagcatatt
     4621 ccataccgga accggcgctt ctttccagcc ttcttgctgc cagttgttca ttgcactgtc
     4681 gttcacgggg cgcgttagcg aaccaaactg ggtatctgcc agaacactat cggtgttaaa
     4741 agatgtaggg atcaggacgc gaaggcgatg atcgtcagcc tggttatcaa ggttgatatc
     4801 cacatcaata cgtctgctgt tatgactcag agtgacaacc atctccaccc ctactctgcc
     4861 cgtggattgc cggacgcttc gttccaacaa attgagcggc accgccatgt catagcggat
     4921 aacagccctg ctctgccagg cctcatgagt aatctcgcat tgcggtttcg ctgttgcaga
     4981 agtcattacc cactcttctt ttgcgggtga atagtcatat tcatcaccat catcagagct
     5041 ttcttcaatt tgcaacaccc ggtcatagcg cacaccgcta tctttatcta ccagttgtag
     5101 cgttccatcc tcattgagcg caatttgcca gaaagcattt tccagtatcc cttcagcgtc
     5161 actttttgcc gcaatcacgt tgccaggctg attcgcttcg atataaagcg tgcgatagcc
     5221 catagaaggg acaatctggt tgatctgtat atcaaactcc ataaagggat cgtaattacc
     5281 gtaatgaact atttgccgat cgattaggcc tggatcgatc tcacgggcat ggcgaataaa
     5341 atacggtaca ggctgaccgc gatcgtctcg tagattaaac tggctggcgc gcagccgcac
     5401 agtggtgtta ataacttctt cccgcggcca gggcatcagg ttaaacagca cgagtttgtc
     5461 ggcatcactc tgtggcatgt tatcggcaat tttgcgcatg tagaaacgca gcagattatc
     5521 cgccatgtct tcagccagtt cgaagcgggc gacaatttcg cgatgaactt tgtcactgca
     5581 acaacaaccg atactgtcgt gggcatgatt ttttaagatc tctttccaca ttttctccag
     5641 caagccgtgg tggtattcaa aacccaacgt ccaggccagc gttgccagcg gttccagcag
     5701 attaacaatc ttattttcaa tacgtgcgtg ggcaattttg atatccatgc gcgtggaacc
     5761 gatggtgcga tgtacgcgca tatatttgcc atcgataaat tcacctttca gggtggccag
     5821 actttcacgc tgcgcttcga tcttctcaaa tacctcttca aagcggctca tcacaaactt
     5881 acgttgaggg tagatctcac gtagcttatc catcacttcg aagatatttt gctgcaatgg
     5941 catctggtca tgcccattcg gcagcaaaat ctctttggtt acagacgctt tttccagcac
     6001 gtcaaaataa ctgtcgaggc gtttacgtaa tccgttttcg tcggcaggta agtacttacc
     6061 gatggcgtag cccagcggca gcacctgcgc cgtcacttcg ctaccgtcac tgctttgcca
     6121 caaaaactcg gttttatcag taccgtggcg ctccgaacat ccgcgccaga acatggtgcg
     6181 ggtaatgcca aatccattgt agatatgcgg cagttgcccg gacatgccaa aggaatctgg
     6241 taaataacct attttcatcg gctcgccaaa cgcgaggcag tcacgcattc cgtacatcag
     6301 attacggacg atggattccg cagaaacaat cgtggtatcg gtctgggtat accaggggcc
     6361 gataatcaac ttgccggctt ctacctgttt cttcacgcgg tctttgtttt ccggtttcac
     6421 cgcgaaataa tcttcgagga tcgccgtttg cccgtcgagt acgtaatatt tgtattcgtt
     6481 gtcctgttcc agtcggcaca ggatctcttc catattattg accagcagaa tgcgtgactc
     6541 ttcggtggtg aaataccact cccgatccca atgcatatgc ggggtgatgt gaacgcgaga
     6601 taccgctttc atcgtcgttt cctttttgtt tatggcatta cgccatcagt cagatagttg
     6661 ccatgcttaa ccgcgtgacg ccgccagatc aggagaattg cagtcgagat tgcagccccc
     6721 accaatgccg caccaaacca gccaattgcc gccataacac cgcccgcgcc attatcatga
     6781 agtaaaaaga gcgagaaaat gccggcaccg ggtgtcgaaa ggccgatatt catcgcaccg
     6841 acaatagcgc ccgttaccat agagcccagc acaaacgaac caataacccg cagcggatct
     6901 tcaatcgcca tcgggatcgc cccttcggta atacctgcca gccctaacag ccaggtggat
     6961 ttcccggtct caatttcaaa ctctttaaac aggcgcggtg cgagcatcgt ggaagcggtt
     7021 acggtaaatg ccgaaaccat tttgacggag gcgaaaatgg catacgggcc gtaaacgccg
     7081 ttcgccattg cgcccaggca gaatgcataa gcggctttgt tcactggccc tccaaggtca
     7141 aaggaacaca taaaaccgag aatggcaccc agcaacagcg cgttacttcc tgacagaccg
     7201 ttcagccagg cggtaagcga gttattgatc caggcgacag gttctcccac cacaaacagc
     7261 atcagactgc ccgctcccaa cgtaccgagc accgggtaga gataaaaagt caggaatcca
     7321 ttgaatttac tgctaagacg caagtgattt ttcacccagc gcatcaagta accggctatc
     7381 aatccgccaa cgaccgcgcc gagaaacccg gagccgatca tgttggcggc aagtccagcc
     7441 gcaaagcctg gcgctaacgc cggtttatct gccagagaat aggcggtata ggccgcgagc
     7501 accggtacca tcaaaattcc gagcagcccg ccgcccagct tgcggtacat ccacagccag
     7561 gagttttctt cattaaacag atcttgtagc ccgaagattt gcgacagtaa taccgcgacc
     7621 gccagcaccg tgccccccgc gacaatcaac ggtacggcaa aagagattcc gctcaacagt
     7681 gcctgtttca gctccgtttt gacacttttc accggttgcg tatcttgctg tacggtacgc
     7741 gtctcatcgc tacgcttgag ggtaagcgct tgttggatca acgcttctgc atggcgaatc
     7801 ggctcggcaa caggcactga aagcgcggga atgccattaa aacgctcact ctccttgatg
     7861 gcgacttcag ccgcaaaaat acaggcggtt gcactattga gttgatccgc cgttaaacgc
     7921 ccttcaatgc cgttagcgcc ttgtttttca acgtaaacat ttacgccgag tttgcgtccg
     7981 gctttttcca gatattccgc agccatatag gtgtgagcaa tacccgccgg acaggccgtt
     8041 acgcagacga tagttggcgc gttggaaaaa gaggcagaag gttgcgtgcc tcccttgtca
     8101 tccagcgccg agagcaactc atcaggcgtc gtcgccgact gtatacgcgc ccgaatctca
     8161 tcatccgcca ggcgcgtggt cagcgctgtc agcagttgca tatgcgtagt acccgcttca
     8221 ttggggggaa tcgccagcag caccactaaa tcaaccgctt ccgggccatc aacgccttcc
     8281 cactgaagcg gctcgctgag tgtggcgacc gcaaacgccg cttcttttac cgcagcagtt
     8341 ttgccatgcg gcacagccaa cccttcacct aaggcggtcg ggccaaggct ttcacggcga
     8401 tacacttctt ccagaaattg ctcagtactg gaaattttcc ccagagcagc aagacgttga
     8461 gtcaacgcgt ggatggcctc ttcacggctg gtaaagcgcg cattcagaca caacgcatcg
     8521 cggtgggtca gagtcgtcag attcatcatg atcctcactt gatcgttttt atagtcgcgc
     8581 cagtgtgccc gataaaacaa taccattatg tgatcaattt cacttgattg aaattatttg
     8641 tattgtattt gtattattta caggatgaat ttatcgggca taatggctaa attcgtatta
     8701 atgagatact aaaaatggga cacaagccct tataccggca gattgccgat cgcattcgtg
     8761 aacaaattgc gcgtggcgag cttaaacctg gcgatgcgtt acctaccgaa tcggcattac
     8821 aaacagagtt tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag ttagtcgagc
     8881 aacagatcct cgaaagcatt cagggcagcg ggacttacgt caaagaagag cgggtcaatt
     8941 acgatatttt tcagttaacc agttttgatg aaaaactgtc ggaccgtcac gtcgatacac
     9001 acagtgaagt cctgatattt gaagtgattc cggctgacga ttttcttcag caacagctac
     9061 aaatcaccgc gcaggatcgt gtgtggcatg tgaagcgcgt gcgctatcgc aagcaaaagc
     9121 caatggcgct ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc acctggcagg
     9181 tcatggaaaa ttcaaaatat cactttattg aagaagtgaa gaaaatggtt atcgatcgta
     9241 gcgaacagga aatcattcct ctgatgccaa ccgaagagat gagccgtctg cttaatatca
     9301 gccagacaaa gccgattctt gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt
     9361 ttgaatatag ccgcaacgct tttaataccg atgactataa atttaccctg atagcgcaac
     9421 gaaaatcatc gcgataagca caaaaaaggc catcatacga tggccaacca tgtcgaaacc
     9481 gacagccctt cgccattcct gtagagggaa gcggcgaggg ctatttctta ttacagatat
     9541 ttatttcaga acagttttca gtgcttcacc gatatccgcc aggctgcgaa cggttttcac
     9601 gcctgcggct tccagagcag cgaatttctc atccgcagtc cctttcccac cggcaatgat
     9661 ggcacccgcg tggcccatac gtttgccttt cggcgcagtc acaccagcga tgtaacccac
     9721 aactggcttg gtaacatgct ctttgatgta cgctgccgct tcttcttcag cgctaccgcc
     9781 gatctcaccg atcattacga tcgcttcggt ctgcggatct ttttcgaaca tttcgagaat
     9841 gtcgataaag ttagagccag ggatcgggtc accgccgata ccaacacagg tcgactgacc
     9901 gaaaccgtaa tccgtggtct gtttaaccgc ttcataggtc agtgtaccgg aacgggaaac
     9961 gatacccact ttacccggtt tgtgaatgtg acccggctgg ataccgattt tgcattcacc
    10021 cggagtgata acgcccgggc agttcgggcc gatcatacga acgccggctt cgtccagttt
    10081 cactttcaca gtcagcatat ccagcgtcgg gatgccttca gtgatggtga taatcagttt
    10141 gatgcctgca tcaatagctt ccagaatgga gtctttgcag aacggtgctg gtacgtagat
    10201 aacagaagcg gtagcgccag tagcagcaac agcttcacgc acggtgttga acaccggcag
    10261 gccgaggtgg gtagtaccgc ctttacctgg ggttacgccg ccaaccattt tagtgccgta
    10321 tgcaatggcc tgttctgagt ggaaagtccc ctggctacca gtaaagccct ggcagataac
    10381 cttggtgttt ttatcaatta aaatggacat tatttcccct ccactgcggc aacaacctgc
    10441 tgagctgcat ccgtcagacc ttttgctgca ataatattca ggccgctgtc agccagtttc
    10501 ttcgcgccga gttcggcgtt gttaccttcc agacgtacca cgaccggtac gttaacaccc
    10561 acttctgcta ccgcgccgat gataccgtca gcgatcaggt cgcaacgaac gataccgccg
    10621 aagatgttaa ccagaacggc tttcactttg tcgtcagaga ggatgatttt gaacgcttcg
    10681 gttacacgtt ctttggttgc gccgccgcca acgtcaagga agttagccgg ttcgccgccg
    10741 tgcagtttaa cgatgtccat cgtacccatc gccagacctg cgccgttaac catacaaccg
    10801 atgttaccgt ccagcgcaac gtagttcagt tcccactgtg cagcctgtgc ttcacgcgga
    10861 tcttcctgcg actggtcacg catttcgcgc agatcaggct ggcggaacag tgcgttgccg
    10921 tcagcgccca gtttgccgtc gaggcaaatc agatcgccct gtttggtgat gaccagcggg
    10981 ttgatttcga tcaacgccag gtcgcgctcc aggaaaatgg tcgccaggcc catgaagatt
    11041 ttggtgaact gctgaaccag tttaccttcc agacccagtt tgaacgccag ctcgcgtccc
    11101 tgatacggca tcgggccagt cagcggatca agcgcaactt tgtggatcag gtgcggagtt
    11161 tcttccgcca ctttttcgat ttccacgccg ccttcggtgg aggccataaa gaccacacga
    11221 cgggaactac ggtctacaac ggcaccgaga tacagctctt tagcgatatc ggtcgctgct
    11281 tcaaccagaa tctggttaac cggttggcca ttggcatctg tttgatacgt taccagacgc
    11341 ttgccgagcc agttttctgc aaaagcacgg atgtcttctt tgctgtttac aactttcaca
    11401 ccgcccgctt taccgcggcc accagcgtga acctgacatt tcactaccca cgggccggca
    11461 ccgatttttg aagcggcttc ttctgcttcg cgcggagtag tacaggcata acccaccggt
    11521 gctggtaagc catagcgggc aaaaagttgt tttgcctgat attcatgtaa gttcatgtgt
    11581 tctgtccatc cttcagtaat cgttatcttt taaaccgtag accgacaccc tatccacgtc
    11641 ggtctacagt gcaggtgaag cttaaactac tacacgtcca gcagcagacg cgtcggatct
    11701 tccagcaact ctttgatcgt taccaggaag cccacggatt cgcgaccatc gatcagacgg
    11761 tgatcgtagg acagcgccag gtacatcatc ggcaggatct caacctgacc attcaccgcc
    11821 atcggacgat ctttgatagc gtgcataccc agaattgcgc tctgcggcgg gttgatgatc
    11881 ggcgtagaca tcagggaacc gaacacacca ccgttggtga tggtgaagtt accaccggtc
    11941 agatcttcaa cggtcagctt gccgtcacgg cctttgactg ccagctcttt gattttcttc
    12001 tcgatgtctg ccatgccgag ggtatcgaca tcacgcagta ccggtgtcac caggccgcgc
    12061 ggcgtagaaa ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc atcgccgtcg
    12121 atagacgcgt tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt cacgtagaag
    12181 gacataaagc ccagacggat gccgtggcgt ttttcaaacg cttcaccgta ctgcttacgc
    12241 agatccataa tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc ggtggagttt
    12301 ttcgcttcca gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg gacacgtttt
    12361 tcactacgtg cagccagagc cggttgcgcc gccggagcag ccgctgccgg agcagactct
    12421 ttcgccgggg ctttcgccag atgtttttcc acatcttcac gagtcagacg cccacccaca
    12481 ccggtgcctt taatggcgct ggcgtcgaga ttgtgttcag ccagcaggcg acggatcgcc
    12541 gggcttaacg catcgttgtt ttgctcttcc agagatgcct gctggcgttg cgccggagtg
    12601 gacgctttct cttcagattt ggcgctggtt tctttaccgg cgctgttgcc ttcacgcagg
    12661 cgaccaagga tctgacgaga cgttaccgtt gtaccttcat cttccagaac cgcatccaga
    12721 atgccgtctg ctgatgccgg tacttccagt accactttgt cagtttcgat ttctaccagc
    12781 acttcatcac gtacgactgc gtcgccgggt tttttatgcc aggttgcgac ggtggcatcg
    12841 gctacggatt caggcaggtc agggaccaga atatctacgc tactcattgt gtatccttta
    12901 tttattcgac gttcagcgcg tcattaacca gatcttgttg ctgtttctgg tgaacggaca
    12961 tataccctac cgccggagag gcggaggccg ggcggcctgc ataacgcaga gaagccccaa
    13021 acggaatcac ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg ttgagcggct
    13081 cttcctggca ccagacaaaa tccttgacgt gagcaaactg ctgcaacact tcctgcatcg
    13141 ctttatgcgg gaacgggtag agttgctcga tacgcacaat ggcgacatcg tgttgattgt
    13201 tcttacgacg ctgttccagc aggtcgtaat aaaccttacc agaacacatc actacgcgct
    13261 tcacgccctt cggatcaagc tcgtcgattt caccgatggc tggcaggaag gtgccgttcg
    13321 ccagttcttc gaggctggaa accgccagcg gatgacgcag cagggatttc ggcgacatca
    13381 cgaccagcgg acgacgcatc ccgcgcagcg cctgacgacg cagcatgtgg taaacctgtg
    13441 ccggggtaga cggtacgcac acctgcatgt tttgctcagc acaaagttgc agataacgtt
    13501 ccagacgcgc ggaggagtgc tccggcccct gcccttcgta accgtgcggc agcaacatca
    13561 ccagaccaca catccggccc catttctgct cgccagagga gatgaactgg tcgataacca
    13621 cctgcgcacc gttggcgaag tcaccgaact gcgcttccca gatagtcaga gtgcgtggtt
    13681 ctgcggtggc ataaccgtat tcaaacgcca gcactgcttc ttcagacaga acggagtccc
    13741 agacacggaa cgcgccctgg ccgttatgaa tatgttgcag cggcgtgtaa gtggaaccgt
    13801 tagactggtt gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg gagtcttcac
    13861 ccgacaggcg aaccggaatg ccttcgtcaa ccagcgtggc gtaagcgagg ttttccgcac
    13921 cgccccagtc gaacagtttc tcaccggcag ccatcgcctg gcgatcgcca taaatcttgg
    13981 caacgcgaga ctgcatttca actacttccg gcaccgtgct gatgcgtttc gccagctcct
    14041 gcaggcgctt catctcaact ttgttcgggt actcttcgtc ccattcgtgg ttgaggtacg
    14101 gcgaccaggt gaaagagtgc atgttcatcg gacgccactc tgccactacg caatcgccag
    14161 catccagcgc atcgcggtac aggttaacca tctcggtggc atcttccagc gtcgcgactt
    14221 tttcctgctc cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt ttgattttct
    14281 gatacatcag cggctgggtt gcgctcggct cgtcggcttc gttgtggccg tgacggcggt
    14341 agcacaccag gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg agcgccagac
    14401 gggtcacaaa ggcaacggct tccggatcat ccgcgttaac gtggaaaatc ggtgcctgaa
    14461 ccatcttacc gatatcagta cagtacggtg tagaacgagc atccagcgga ttagaggtgg
    14521 tgaaaccaac ctggttgttg ataacgatac gtaccgtacc gccaacttca taaccacgcg
    14581 ctttcgacat gttcagagtt tcctgaacca cgccctgccc ggtcactgcg gcgtcaccgt
    14641 ggatggtgat tggcagcact ttgttgctgc tcggctcatc aagtctgtcc agacgggcac
    14701 gaacagaacc gataactacc gggcttacaa tctcaaggtg agacgggtta aacgccagcg
    14761 ccaggtgcac caggccgcca tcggtctgga agtcagacga gaagcccatg tggtatttca
    14821 cgtcacccgt gccgaggtgt tctttatgtt taccggcaaa ctcgtcgaac aagtcttgcg
    14881 gttttttacc cagcacgttc accagcacgt tcagacgacc acggtgcgcc atcccaagaa
    14941 ccacttcgcg ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc atcgggatta
    15001 acgcgtcacc gccttccagc gagaagcgtt ttgcgccagg gaattttgcg ccgaggtaac
    15061 gttcaaggcc ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct tcgctattga
    15121 aagtcgcgcg accagactcg atacgctgtt ggatccagcg tttttcttcg gtgctggtaa
    15181 tgtgcatata ctcggcacca atcgggccac agtaggtttg cttgagggct tccagcagct
    15241 cgccgagttt catggtttct ttgccgctgg caaatgaacc gacgttgaag gtttcctgga
    15301 agtctgcttc ggtcagatcg tggaaagacg gatccagatc ggccacttta tcttgctgcc
    15361 acagtcccag cggatcgaga ttcgcatgct ggtgaccacg gaagcggtat gcgttaatga
    15421 gctgcaggac tttaacctgc ttcacattgg tgtcagggtc ggagatcgtt gaagagtaac
    15481 gtgaagcgtc tttcgccagg cggcggaaat attcacgcgt ttgagagtgg aattgatccg
    15541 gtttgactcc cgtaccaggt aactgctgga acgtcgaacg ccagttagcg tcaaccgagt
    15601 caggatcggt taagaagtct tcatagagct gttctatcca gctctggttt gcgccagaga
    15661 ggtaagaaga gtccaaccag gctttcaaag cgctgttctg catcgtgatc ccttaagcat
    15721 cttttttatg cttacttcgc cgtggatact accacgcaca gtgatgtgcg tgccggggtt
    15781 cacttgcgag ctcttgtcta tgtagtggct cgcgaaggaa cctttaaaaa ctgtcttagt
    15841 gtcaggggca gtttttaaag atttcctgca tttctttgtt acgcctgatg cgctgcgctt
    15901 atcaggccta cgggtaatga cttgtaggcc ggataaggcg ttcacgccgc atccggcact
    15961 ggttgcctga tgcgacgctt gcgcgtctta tcaggcctac ggtttacgca ttacgttgca
    16021 acaacatcga cttaatatgg ccgatggcgc gcgtcgggtt cagccccttc ggacatacac
    16081 tgacgcagtt catgatgctg tgacagcgga atacgctgaa tgcatcgctc aaaccgtcga
    16141 ggcggctgtc agtctcggta tcacggctat cgatcaggaa acgatatgcc gctaacaagc
    16201 ctgccgggcc gataaactta tcgggattcc accagaaaga cggacaagag gttgaacaac
    16261 atgcgcagag aatacattca tacagcccgt cgagtttttc gcgctgctct ggcatctgta
    16321 aatgctcgcg agctggcgga ttttgtccat tattcaacag gtaaggctta attttctcat
    16381 attgcgcata gaattgtccc atgtctacca ccaaatcgcg gatcaccggt aaacctggca
    16441 gcgggcgaat cacaatcttc ttgcccggct ggttgagtgc cgaaatcggg gtaatacagg
    16501 ccagaccatt cttgccgttc atgttcagac cgtcggaacc gcacacacct tcacggcagg
    16561 agcggcggaa cgacaggctg ggatctttct ctttcagctg gataagcgca tccagcagca
    16621 tcatgtcgcg accttcttcc gcttccaggg tgtaatcctg catacgcgga gcatcatcaa
    16681 catccgggtt atagcgataa attgaaaact cgagtctcat tttcctgtct ccgcattagt
    16741 aagtacgaat cttcggcggg aatgccgggc gcagtttcgg ttccatgttg acgcttcggc
    16801 gcgtcatgga ttccgactct ggcagataca gggagtggca cagccagttt tcatcatcac
    16861 gatccgggaa gtcgaagcgg ctatgtgcgc cacggctttc ggtacggaag ttggcagaaa
    16921 cagccgttgc atacgccgtt tccatcaggt tatccagttc caggcactca acgcgctggg
    16981 tgttgaactc gctggaagtg tcatccagac gggcattttt cagacgctcg cggatcactt
    17041 tcaactgctc aagccctttc gccatcgcat caccttcacg gaagaccgag aagttatgct
    17101 gcatacattc ttgcagcgct ttacgaatcg ccaccggatc ttcaccgtta cgattattgt
    17161 tccagcggtt cagacgatcc agagatgctt caacatcaga ctcgctggca tcgcgcaatg
    17221 cgccctgttc ggcgatagac tcttgcagat gcagacccgc tgcacgacca aagaccacca
    17281 ggtccagcag cgagttgccg cccagacggt tagcgccgtg taccgataca caagcgattt
    17341 caccaacggc aaacagtccc ggaacaacca catcttcgcc tttctcgttc accgtcagcg
    17401 cctgaccggt cactttggtc ggaataccgc ccatcatgta gtgacaggtt gggataaccg
    17461 gaatcggctc tttcaccgga tcaacgtgag cgaaggtacg ggagagttcc aggatacccg
    17521 gcaggcggga ttcgagaact tctttaccca ggtggtcgag tttcagtttc gcgtgtggcc
    17581 cccacggacc atcacagccg cgaccttcac ggatttcgat catgatggaa cgcgccacca
    17641 catcacggcc cgccaggtct ttggcgttcg gcgcataacg ctccataaag cgttcgccat
    17701 gtttgttcag cagataaccg ccttcaccac ggcaaccttc ggtgaccagt acgcccgcac
    17761 cggcaatacc ggtcgggtgg aactgccaca tttccatatc ctgcaccggt acgcctgcgc
    17821 ggatagccat gccgacgccg tcgccagtgt taatgtgggc gttagtggtg gactgataaa
    17881 tacgccctgc cccgccagtc gccagcacgg tagcgcgggc tttgaaataa accacttcac
    17941 cagtttcgat gcacagtgca gtacaaccca ccaccgcgcc atcctggttt ttcaccagat
    18001 ccagcgcata ccactcggag aaaatagtgg tgtggttttt caggttctgc tgataaaggg
    18061 tatgcaacag tgcgtgaccg gtacggtcag ctgctgccgc agtgcgtgcc gcctgctcgc
    18121 cgccgaagtt tttcgactga ccgccaaacg gacgttgata gatacgacca tcatcaagac
    18181 gcgagaacgg caggcccatg tgttcgagtt ccagaatcgc ttccggcccg gttttacaca
    18241 tatattcaat cgcgtcctgg tcaccgatat agtccgaacc ttttacggtg tcgtacatat
    18301 gccattccca gttatcttca tgggtattac ccagcgcaac ggtaatgccg ccttgcgcag
    18361 aaacggtatg ggaacgggtc gggaagactt tagagagcag cgcacaggtt tgaccgctct
    18421 gggaaatttg cagcgccgcg cgcatacctg cgccaccggc accaatcaca actgcatcaa
    18481 attctctgac tggcaatttc atcacacacc ccacaccaca acgaatccat aaatcacgta
    18541 aaccaccaga gcaacgacaa tcaccagttg cagcatcagg cgcaaggcca gcggtttaac
    18601 gtagtcggtc aacacctgcc acatgccgat ccaggcatgg atcaagatag aaaacagcgc
    18661 cagcagggtg aacactttgg tgaacgcaga ggcgaagaaa ccgatccaga cttcatatgt
    18721 cagctcgcca ctggtagcga aaaaaccgac catataaatg atgtagagcg tcaggacgat
    18781 agcggtagcg cgaacgagga tgaaatcatg tacgccattg cgtcctaatg cggaggcgtt
    18841 gcttaccata cgaggactcc tgcgagaagt gaaagcacga cagtaataac aaaggagatt
    18901 ttggcggagc gtttacccgc ttcgaatgtt tcttccagat agccaaaatc catcatcatg
    18961 tggcgaatac ctacgacgac gtgatacgcc agagcggtaa ggatgcccca catgataaat
    19021 ttgacgaaga agctgcccat aatcgcggaa gcttgctcga aaccttcagg ggaagagagg
    19081 ctggtaccca gaagccacag caggataccc actgcaataa aggtgatcac accggaaacg
    19141 cgatggagaa tggacgctat cgccgtgatg gggaaccgga tggtctgtag gtccagatta
    19201 acaggtcttt gttttttcac atttcttatc atgaataacg cccacatgct gttcttatta
    19261 ttccctgggg actacgggca cagaggttaa ctttctgtta cctggagacg tcgggatttc
    19321 cttcctccgg tctgcttgcg ggtcagacag cgtcctttct ataactgcgc gtcatgcaaa
    19381 acactgcttc cagatgcgaa aacgacacgt tacaacgctg ggtggctcgg gattgcaggg
    19441 tgttccggag acctggcggc agtataggct gttcacaaaa tcattacaat taacctacat
    19501 atagtttgtc gggttttatc ctgaacagtg atccaggtca cgataacaac atttatttaa
    19561 tttttaatca tctaatttga caatcattca acaaagttgt tacaaacatt accaggaaaa
    19621 gcatataatg cgtaaaagtt atgaagtcgg tatttcacct aagattaact tatgtaacag
    19681 tgtggaagta ttgaccaatt cattcgggac agttattagt ggtagacaag tttaataatt
    19741 cggattgcta agtacttgat tcgccattta ttcgtcatca atggatcctt tacctgcaag
    19801 cgcccagagc tctgtaccca ggttttcccc tctttcacag agcggcgagc caaataacaa
    19861 acgggtaaag ccaggttgat gtgcgaaggc aaatttaagt tccggcagtc ttacgtaata
    19921 aggcgctaag gagaccttaa atggctgata caaaagcaaa actcaccctc aacggggaca
    19981 cagctgttga actggatgtg ctgaaaggca cgctgggtca agatgttatt gatatccgta
    20041 ctctcggttc aaaaggtgtg ttcacctttg acccaggctt cacttcaacc gcatcctgcg
    20101 aatctaaaat tacttttatt gatggtgatg aaggtatttt gctgcaccgc ggtttcccga
    20161 tcgatcagct ggcgaccgat tctaactacc tggaagtttg ttacatcctg ctgaatggtg
    20221 aaaaaccgac tcaggaacag tatgacgaat ttaaaactac ggtgacccgt cataccatga
    20281 tccacgagca gattacccgt ctgttccacg ctttccgtcg cgactcacat ccaatggcag
    20341 tcatgtgtgg tattaccggc gcgctggcgg cgttctatca cgactcgctg gatgttaaca
    20401 atcctcgtca tcgtgaaatt gccgcgttcc gcctgctgtc gaaaatgccg accatggccg
    20461 cgatgtgtta caagtattcc attggtcagc catttgttta tccgcgcaac gatctctcct
    20521 atgccggtaa cttcctgaat atgatgttct ccacgccgtg cgaaccgtat gaagttaatc
    20581 cgattctgga acgtgctatg gaccgtattc tgatcctgca cgctgaccat gaacagaacg
    20641 cctctacctc caccgtgcgt accgctggct cttcgggtgc gaacccgttt gcctgtatcg
    20701 cagcaggtat tgcttcactg tggggacctg cgcacggtgg tgctaacgaa gcggcgctga
    20761 aaatgctgga agaaattagc tccgttaaac acattccgga atttgttcgt cgtgcgaaag
    20821 ataaaaatga ttctttccgc ctgatgggct tcggtcaccg cgtgtacaaa aattacgacc
    20881 cgcgcgccac cgtaatgcgt gaaacctgcc atgaagttct gaaagagctg ggcaccaaag
    20941 atgacctgct ggaagtggct atggagctgg aaaacatcgc gctgaacgac ccgtacttta
    21001 tcgagaagaa actgtacccg aacgtcgatt tctactctgg tatcatcctg aaagcgatgg
    21061 gtattccgtc ttccatgttc accgtcattt tcgcaatggc acgtaccgtt ggctggatcg
    21121 cccactggag cgaaatgcac agtgacggta tgaagattgc ccgtccgcgt cagctgtata
    21181 caggatatga aaaacgcgac tttaaaagcg atatcaagcg ttaatggttg attgctaagt
    21241 tgtaaatatt ttaacccgcc gttcaaatgg cgggttaatt tttatatgcc taaacacaaa
    21301 aaattgtaaa aataaaatcc actatcggac attcatatat tctaaaaaaa aaatatatcg
    21361 gtataaatta taaacaaatc gatgattttt aatcatctat cttcct
//
LOCUS       DAZGXZ010000085        21128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000085 DAZGXZ010000000
VERSION     DAZGXZ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.085"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..173)
                     /locus_tag="ACHKJF_003307"
     CDS             complement(<1..173)
                     /locus_tag="ACHKJF_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0950616.1"
                     /translation="MANRKQQRARAERLHIRSEINRRLFRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYL"
     gene            497..1021
                     /locus_tag="ACHKJF_003308"
     CDS             497..1021
                     /locus_tag="ACHKJF_003308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950617.1"
                     /translation="MWRINITWQLVIVCIRGVFLMSDKLPSDYDILFDLWYSYWLERI
                     TCVFYRRVSAALRISAFLVGVAAFVLCDFVVLVLFSVVLFLLWQCMAASFERRAEKAE
                     WQTVSYQRLISQRHVISKECMIARLSFVEESDSPIMECMINLAWNKACEAVGSSSRNH
                     VSISGRLLSRLCGF"
     gene            complement(995..1345)
                     /locus_tag="ACHKJF_003309"
     CDS             complement(995..1345)
                     /locus_tag="ACHKJF_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0950618.1"
                     /translation="MAVIYIAGPMTGCKDYNRTAFCAEDLKLRCAGNIVLNPAVLPDG
                     LSQQQYMSICIPMLMCADAIYLLDGWEESAGARAEYAMALKLNMPVSFPENRAAGETV
                     AQLFGKSKAAQATE"
     gene            complement(1336..1554)
                     /locus_tag="ACHKJF_003310"
     CDS             complement(1336..1554)
                     /locus_tag="ACHKJF_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950619.1"
                     /translation="MIQESTVIRFTANGRQYEVDESLIDPGMTRQGSRNPEMHHIRLI
                     NGSHFCATNVEEVRVLTATRNTGGKAWQ"
     gene            complement(1551..1787)
                     /locus_tag="ACHKJF_003311"
     CDS             complement(1551..1787)
                     /locus_tag="ACHKJF_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950620.1"
                     /translation="MNSENNKELLRELRLIARAHEETAKVVAAAGRPELVITSAILLR
                     ITMEAIRVIQDQESEIAALKKQLNEKSAGQRGQQ"
     gene            complement(1823..2803)
                     /locus_tag="ACHKJF_003312"
     CDS             complement(1823..2803)
                     /locus_tag="ACHKJF_003312"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFP0950621.1"
                     /translation="MSKELLSGTPLKWVGGKSRIMNTLKAHLPEADCLVEPFVGGASV
                     FMNTSYKRYILGDSNGALINFWRAARDNTDSLIDEAKTLFLNHNNRGDYLIIRETFNA
                     ENHAFLSSRTGISDRRELRLAAMFLYLNRHCFNGLYRVNGKGDFNVPFGGYRKPYFPE
                     REIRAFADKANSTQTLLIHADFKDTLSSASHLFGMGNTLCVYCDPPYLRFSGEGNFTA
                     YGKPFDEHEHVRLRAALDRLSQETGGMTRITISNSDTPETRRIYRGYRMNCIQAPRSV
                     GARTREPAPEVIATLRQCNACGHYGGGYCPDCGPVTGDATYSVMFTPAST"
     gene            complement(3037..3618)
                     /locus_tag="ACHKJF_003313"
     CDS             complement(3037..3618)
                     /locus_tag="ACHKJF_003313"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0950622.1"
                     /translation="MNHLMIDTETLGNGPDAAIFAIGAVFFDPFTGKLGKQFEKFIDP
                     VDSERNGGTVNAATAVWWAGQSVEARACLRNADGTELAAVTEFLAFISRNMHDESPGN
                     SLTIWCKGASFDFPILKSAITRTAGEKSIPWCYWDERCMRPLIAMAESTGWRMPGRSG
                     KETIAHTALGDAVYQAKVVSEIWQRFTTPFLNI"
     gene            complement(3618..3848)
                     /locus_tag="ACHKJF_003314"
     CDS             complement(3618..3848)
                     /locus_tag="ACHKJF_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950623.1"
                     /translation="MRTAESPGRKITARPANHHAHISMAARKAERAHICELLRELTET
                     AKETHTCPQETCAAIERLIAILANPDELKGCA"
     gene            complement(4063..4188)
                     /locus_tag="ACHKJF_003315"
     CDS             complement(4063..4188)
                     /locus_tag="ACHKJF_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950624.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIHELKDIELFFTGDKRRK"
     gene            complement(4185..4427)
                     /locus_tag="ACHKJF_003316"
     CDS             complement(4185..4427)
                     /locus_tag="ACHKJF_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0950625.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVAA"
     gene            complement(4446..4781)
                     /locus_tag="ACHKJF_003317"
     CDS             complement(4446..4781)
                     /locus_tag="ACHKJF_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0950626.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATATTEIHTTPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLISGL
                     RFQYPGSKH"
     gene            complement(4807..5001)
                     /locus_tag="ACHKJF_003318"
     CDS             complement(4807..5001)
                     /locus_tag="ACHKJF_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950627.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            complement(5053..5577)
                     /locus_tag="ACHKJF_003319"
     CDS             complement(5053..5577)
                     /locus_tag="ACHKJF_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0950628.1"
                     /translation="MRKSIADKELSDTQALQENDSITESVGAANDDEHQEEKKKRTYK
                     KGATLRLDGPVAGLCSLEKGAAYIGLTKAALRVALQRNQMPGHKTRSNPEDENSDGVW
                     WFNAREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     KSKLEQHQYSEGNQ"
     gene            5681..6022
                     /locus_tag="ACHKJF_003320"
     CDS             5681..6022
                     /locus_tag="ACHKJF_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0950629.1"
                     /translation="MVNMSRDYAKKLKAIRNAENLTQSQFSQVTGVSVGTIGNYESGY
                     KPARIEVVEKVIQVERFEKYTMWILHDKTFPAAGQVAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSRRNAG"
     gene            6092..7084
                     /locus_tag="ACHKJF_003321"
     CDS             6092..7084
                     /locus_tag="ACHKJF_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0950630.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIDAWWVLDGRNQPYGDTYRVRLEKINREMRDPRASEMT
                     RKFMLGYRSDKLQAGLMPSSVNRDLCALSAMFTSLIDADVFHNENPVRGIRKLKVRNT
                     EMAFLSDDEIDRLLERLEGDARRVAVLCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDTVLSLIKTRKTGLLYQADYIRFRDILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLADSVHKLSID"
     gene            7384..9828
                     /gene="dmsA"
                     /locus_tag="ACHKJF_003322"
     CDS             7384..9828
                     /gene="dmsA"
                     /locus_tag="ACHKJF_003322"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP0950631.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            9839..10456
                     /gene="dmsB"
                     /locus_tag="ACHKJF_003323"
     CDS             9839..10456
                     /gene="dmsB"
                     /locus_tag="ACHKJF_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP0950632.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            10458..11321
                     /gene="dmsC"
                     /locus_tag="ACHKJF_003324"
     CDS             10458..11321
                     /gene="dmsC"
                     /locus_tag="ACHKJF_003324"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP0950633.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(11357..11983)
                     /gene="ycaC"
                     /locus_tag="ACHKJF_003325"
     CDS             complement(11357..11983)
                     /gene="ycaC"
                     /locus_tag="ACHKJF_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP0950634.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            12297..13445
                     /gene="ycaD"
                     /locus_tag="ACHKJF_003326"
     CDS             12297..13445
                     /gene="ycaD"
                     /locus_tag="ACHKJF_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0950635.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGSLPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            13655..15085
                     /gene="ycaM"
                     /locus_tag="ACHKJF_003327"
     CDS             13655..15085
                     /gene="ycaM"
                     /locus_tag="ACHKJF_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0950636.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLQARKANSK"
     gene            complement(15086..15994)
                     /gene="ycaN"
                     /locus_tag="ACHKJF_003328"
     CDS             complement(15086..15994)
                     /gene="ycaN"
                     /locus_tag="ACHKJF_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0950637.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            16094..16684
                     /gene="ycaK"
                     /locus_tag="ACHKJF_003329"
     CDS             16094..16684
                     /gene="ycaK"
                     /locus_tag="ACHKJF_003329"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFP0950638.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(16766..17506)
                     /gene="pflA"
                     /locus_tag="ACHKJF_003330"
     CDS             complement(16766..17506)
                     /gene="pflA"
                     /locus_tag="ACHKJF_003330"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP0950639.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(17698..19980)
                     /gene="pflB"
                     /locus_tag="ACHKJF_003331"
     CDS             complement(17698..19980)
                     /gene="pflB"
                     /locus_tag="ACHKJF_003331"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0950640.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(20035..20892)
                     /gene="focA"
                     /locus_tag="ACHKJF_003332"
     CDS             complement(20035..20892)
                     /gene="focA"
                     /locus_tag="ACHKJF_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP0950641.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         4927 a         4998 c         5471 g         5732 t
ORIGIN      
        1 agatagctga agacggcagc gcagtaacgg tttgatagtg cgtgcgtgcg ctcatgcagc
       61 atgttgatat gcatgatttg tgcgacgcgt gttgcgcgga aaagtctgcg gttgatttcg
      121 ctgcggatgt gaagacgctc agcgcgggcg cgctgttgct tgcggtttgc catggtgtgg
      181 cctctttgct agtaagttct gaaaactcac caacccgagg ttccaagctc agggtggcga
      241 gacgtacagg gttggaacta ccggctagca aagaacccgg cccgaccgaa gtcggccccg
      301 tacgccccgc cattattctg acgcgaaaaa gacgtggcga tacagtacgc acaaaaaaac
      361 cgctggcgcg gttgtgcgct ttgctagtcg gcggggttcc aatcccggca cccgttttat
      421 gaggtgcagc ggaaatgtaa cctgacacga cgagcgctgg caagcggttt ttgtgttgcg
      481 tgctgatgtg gttagaatgt ggcgaattaa tatcacctgg caactggtga ttgtctgcat
      541 acgcggggtg tttctgatga gtgataagct cccttctgat tacgacattc tttttgacct
      601 gtggtacagc tactggcttg aacgtattac atgtgtgttt taccgacgcg tttctgctgc
      661 tctgcgaatc agcgcctttc tggttggtgt tgcggcgttt gtcctgtgtg attttgttgt
      721 gttggtactc ttcagtgtgg ttctgtttct gctctggcag tgcatggctg ccagttttga
      781 acgcagggct gaaaaggctg aatggcagac agtcagttac cagcgcctta tcagtcagcg
      841 ccatgtgatt agtaaggaat gcatgattgc gcgcctctcc tttgttgagg aaagtgacag
      901 tcccatcatg gagtgcatga ttaatcttgc ctggaataaa gcctgtgagg cggttggcag
      961 ctcttcgcgc aatcatgttt cgatctccgg tcggttactc agtcgcctgt gcggcttttg
     1021 atttgccaaa cagctgtgcg acagtttcgc cggccgcccg attttccggg aatgacaccg
     1081 gcatgttcag cttgagtgcc attgcgtact cagcccgtgc gccagcggac tcttcccatc
     1141 catccagtaa gtaaatggca tcggcacaca tgagcatcgg aatacaaatg ctcatgtact
     1201 gctgctggct taatccgtca ggcagcacgg caggattaag aacgatgttt ccggcgcacc
     1261 gaagtttcag gtcctccgcg caaaatgccg tcctgttgta gtctttgcat ccggtcatgg
     1321 gaccggcgat ataaatcact gccatgcttt acctcccgta ttgcgtgttg ctgtcagcac
     1381 gcgtacttct tccacgtttg tggcgcagaa gtgcgaaccg ttgataaggc ggatgtgatg
     1441 catctctgga ttgcgtgagc cctggcgtgt catgccgggg tcaatcaggc tttcgtctac
     1501 ctcgtactga cggccgttgg ctgtgaatct gataacggtt gactcctgga tcattgctga
     1561 cccctttgtc cggcagattt ctcgttgagc tgctttttaa gggcggcgat ttcactttcc
     1621 tgatcctgaa tcacccggat tgcttccatg gtgatcctca gtaagattgc tgaagtgata
     1681 acgagctctg gtcttccagc agctgcgaca acttttgctg tttcctcgtg tgcacgggcg
     1741 atgagacgca attcccgcaa aagctctttg ttgttttcgc tattcatttt ctgactccgg
     1801 tgttactgat tcatctgttt attcacgtac tggcaggggt aaacatcacg ctgtaagttg
     1861 catctcccgt cacaggtccg cagtccgggc agtagccacc gccatagtga ccgcaggcgt
     1921 tgcactgtct cagggtggcg ataacttccg gtgctggttc gcgggttctt gctccaacgc
     1981 ttcgtggtgc ctgtatgcag ttcatccggt aaccccgata aatgcgtctg gtttccggtg
     2041 tgtcggaatt ggagatggtg attctggtca tgccgcccgt ttcctgcgaa aggcggtcaa
     2101 gtgctgccct caggcgaacg tgctcgtgtt catcgaatgg ttttccgtag gcggtgaaat
     2161 tgccctcgcc actgaaccgc aggtaagggg gatcgcagta cacgcaaagc gtgttgccca
     2221 tgccgaacag gtgactggca gagctcagcg tatccttaaa atctgcgtgg ataagcagcg
     2281 tttgggtgct gtttgccttg tctgcaaatg cgcggatttc ccgctcaggg aagtagggtt
     2341 ttcggtagcc gccgaaaggc acgttaaaat caccttttcc gttcacccgg tacaggccat
     2401 taaaacagtg ccggttgagg tacaaaaaca tggcggccag tcgcagttca cggcggtcgc
     2461 ttattcccgt tctgctactc aggaaggcat gattctctgc gttaaaggtt tcgcgaatga
     2521 tgagatagtc acccctgttg ttgtggttca ggaacagggt tttcgcctcg tcgatcagac
     2581 tgtcggtgtt gtcccgtgct gcccgccaga aattaatcag cgcaccattg gaatcaccca
     2641 ggatgtaacg cttgtaactg gtgttcatga ataccgatgc gccacccacg aacggttcaa
     2701 ccaggcagtc agcttctggc agatgcgcct tcagtgtgtt catgatgcgg cttttgccac
     2761 ccacccattt cagcggtgtg ccggataaca gttctttgct cataacgcgg cctctttgtt
     2821 ataggtttcg tgcgttaata aatcccatga cttcccgttg tttttgctca gtaatcgcca
     2881 gcgaaaaccg attcttatta ccaggtagtc gtggggtttt atacgtgaaa aattccgcac
     2941 gccgtcgcgg aataaacgca gcgatgcgcg ggcttttctt tccacgttca ggggggggca
     3001 gcaacacaca ttcaggatgg cggccatggc ttcacctcat atgttcagga atggtgttgt
     3061 gaagcgctgc cagatttctg acacgacctt tgcctggtaa acggcgtcgc ccagcgcggt
     3121 gtgagcaatg gtctctttgc ctgatcggcc tggcattctc cagccggttg attcagccat
     3181 ggcgataagc ggacgcatac agcgctcgtc ccagtagcac caggggatgc ttttttctcc
     3241 ggctgtgcgt gttatggcgg atttcaggat tgggaaatcg aaggatgcgc ctttgcacca
     3301 tatggtcagt gaattgcccg ggctttcgtc atgcatattg cgtgaaatga acgccagaaa
     3361 ttcggtcact gctgccagct ctgttccatc tgcgttgcgc aggcaggctc tggcttccac
     3421 ggattgtccg gcccaccata cagcggtggc tgcattgacg gttccgccgt tgcgttctga
     3481 atccaccggg tcgatgaatt tctcaaactg cttgcccagc tttccggtga acgggtcgaa
     3541 aaacaccgcc ccgatcgcga atatggctgc gtccggtccg ttacccagtg tttcggtgtc
     3601 aatcatgaga tggttcatta tgcgcacccc ttcagttcgt ctgggttggc gagtattgca
     3661 atcagtcgtt caatggctgc gcaggtttcc tgtggacagg tgtgtgtctc tttcgctgtc
     3721 tcagtcagct cgcgcagtaa ttcgcagata tgcgcacgct cggcttttct tgcggccatg
     3781 ctgatatgtg catgatggtt agctggtctg gcggttattt ttctgccggg tgattcggct
     3841 gttctcattt gttgactcct gaaaaaagac aaagggaacc cgaaggcatg aatgccttaa
     3901 ttaattgctc gtgtgttttc tggtgttatt ccgcgcctgc tttcggcttc agattatccc
     3961 cagattcttc agtgctaacc agataagcgc actcacctga aatatcaccc atgcagtagc
     4021 gatggtgagt attactttta cattcttcat aaagttttcg ctttatttac ggcgtttgtc
     4081 gccagtaaaa aataattcga tatccttcag ttcgtggata attcgcataa tgccgattgc
     4141 cataagcacg gagataatca gaattaccca tgaaagaaat atgctcatgc ggctacccct
     4201 gtgaagtaag gctcaacaat ttcaccttca aacagttctg tcataatcga ctttaattct
     4261 tccagcgctt cacggtcatc gctgcccagt gcgcagttgt atagccccat aattgcgccg
     4321 cgcagtgagt tacgaaggct ttcatcctta gttacgcccc ggtttgcgag ttcaaagtga
     4381 taacgttgca acgcgttatt gataagtatt ttgtaggctt cgatcatggc tgaatccttt
     4441 atttatcagt gcttgcttcc cggatattga aaacgcaggc cgctgattaa tctgtctgcc
     4501 gttcttaagg cttcaaaaaa tgtgaagtcc tgtccgaagt aatcatcccc gcgagtcagg
     4561 gtgaaattaa atctgccagt aacagggctg cgaaaacttt tgtgaataac gatgccagtg
     4621 tcgccaatcg cccaggtgtg ctttccggtt tgttttaccg ggtgaccgtc tggtgtggta
     4681 tggatttcag tggttgcggt cgcactattt ttacggaagt gctggcgtgc aacgtggcgt
     4741 tcggtgttgt tattgatgcg tgcatttcgt tgttgcttca ttcttcactc ggtccttatg
     4801 cgatgattac agtgattcgt ctcttgtcag cctaaaaatc acttcagcca tgacttcgct
     4861 aattacatcg atcccctgat cggtaagggc ttctttccag gccttgcttg cacgtgttgg
     4921 atccatcctg cctgattgct gttgtatgat gatgaagcgt aaatcgctgt ataagtcttt
     4981 tgtttgagtg gagtcagtca tgtcaacacc ttcaaatccc gatgctctta atgatcccgg
     5041 tttgttttga tgtcactggt tgccttcgct gtattggtgt tgctccagtt ttgactttct
     5101 tcctgtgtaa acccgctttc cattgatggt ctggcagtct tctttgtttg ccggggaaaa
     5161 ctttcttttc tgtcggtcgt acgtccagta gcttttccag ttgtgccatt ctggcggctc
     5221 gcattctggt agttcgtcag ccagcttgtc ccactctctg gcgttaaacc accatacgcc
     5281 atccgaattt tcatcttcag ggtttgaacg ggtcttgtgt ccaggcatct gatttcgctg
     5341 aagcgccacg cgcaatgcag cttttgtcaa cccgatatag gctgcccctt tttcaagtga
     5401 gcaaagtccg gctaccgggc catccagtcg caaggttgcc ccttttttgt aggtgcgttt
     5461 tttcttttcc tcttggtgtt catcgtcgtt tgccgcgcca acggattcgg tgatgctgtc
     5521 gttttcctga agggcttgtg tgtcagataa ctctttatcg gcaattgact tccgcatttg
     5581 ctatcctctt gcgttggtgc gcttgtcgcg catcatgaag gtttgttttg atttgtttac
     5641 ttcaccctgt ggtgaagttc gaatagagga tactcatcgt atggtgaaca tgtcaagaga
     5701 ttacgccaaa aaactaaagg caatcagaaa cgcggaaaac cttacacaat ctcagttttc
     5761 gcaagtaacg ggggtttctg taggaactat aggaaattat gagtctggat ataaacctgc
     5821 gcgaattgag gttgtagaaa aagtaatcca agtcgagcga ttcgaaaaat acactatgtg
     5881 gatacttcac gacaaaacat ttcccgctgc cgggcaggtt gccccggctc tctctcttga
     5941 tggctccatt cagtcggagg gcgatcaggt ttcaatcggc attacccaaa aatcaccccg
     6001 atctcgtcgc aatgctggct aacgctgtac atcgagcggg tttcatcccg tagagagtgt
     6061 acggctgatt catccgttgg aggggctaat catgtcgatt aagcaactca aagacgggcg
     6121 ttataaggtt gatgtaagac cgcaagggac ggaaggaaag cggatccgta agatttttac
     6181 cctgaagtca aaggcacagg agtttgaaag gtatgttttg cagaatttcc acgataaacc
     6241 gtggcaggcc aagccagctg atcagcgtcg gttatccgaa ctgatagatg cctggtgggt
     6301 gcttgatggc cggaatcagc cttacgggga tacttacagg gtcaggctgg aaaaaataaa
     6361 ccgtgagatg agggaccccc gcgccagtga gatgacgcga aagtttatgc tcgggtatcg
     6421 ttcagataaa ttacaggccg gattaatgcc ctccagtgtt aatcgtgatt tgtgtgcctt
     6481 gtctgcaatg tttacctcgc tgattgatgc ggatgttttc cataacgaaa acccggtgcg
     6541 tggaatacgt aagctcaagg tcaggaatac agaaatggcc tttctttcag atgatgagat
     6601 tgatcggctg cttgaacgac tcgaggggga tgcgcgtcgc gttgctgttc tgtgtctttc
     6661 cactggcgca aggtgggggg aagcagctgg cttacgtggt gagcatatcg ttggtaaccg
     6721 cgtaatgttc tttaacacaa agaatggaaa atcccgcgcg gttcctgttt ctgatacggt
     6781 gctttcgttg ataaaaaccc gaaaaaccgg gctattgtac caggctgatt acatccgttt
     6841 tcgggatatc cttcaggagg tgaaaccgga tttacccaaa ggtcaggcga ctcacgttat
     6901 gcgtcacacg ttcgccacgc atttcatgat gaacggcgga aatatcgtta cgttacagcg
     6961 aatccttggg cacgccacga ttcaacaaac gatgacgtat gcgcattttt caccggactt
     7021 tttacaggat gcgattagct ttaatccgct ggctgatagt gtccataaac tgtccattga
     7081 ctagttgtaa tttgcgttaa tttgctcccc ttgttttgat ttagtcattg aaaaataagt
     7141 aaatcatcaa ggggagtaag gtgtgttaaa tctcctgcgg gcgctttttt tgtctccctt
     7201 tgataccgaa caataattac tcctcactta cacgtaatac tactttcgag tgaaaatcta
     7261 cctatctctt tgattttcaa attattcgat gtatacaagc ctatatagcg agctgctata
     7321 gaaataatta cacaatacgg tttgttactg gaatcaatcg tgagcaagct tgagtgagcc
     7381 attatgaaaa cgaaaatccc tgatgcggta ttggctgctg aggtgagtcg ccgtggtttg
     7441 gtaaaaacga cagcgatcgg cggcctggca atggccagca gcgcattaac attacctttt
     7501 agtcggattg cgcacgctgt cgatagcgcc attccaacaa aatcagacga aaaggttatc
     7561 tggagcgcct gtacagttaa ctgtggtagt cgctgcccgc tacgtatgca cgtcgtggac
     7621 ggtgaaatca aatatgtcga aacggacaat accggcgatg acaattacga cggcctgcac
     7681 caggttcgcg cctgcctgcg tgggcgttcc atgcgtcgcc gtgtctacaa tccggaccgc
     7741 ctgaaatatc cgatgaaacg agtcggggcg cgcggtgaag gcaaattcga gcgcattagc
     7801 tgggaagaag cctacgacat catcgcgacc aatatgcagc gcctgatcaa agagtacggc
     7861 aacgagtcta tctatctgaa ctatggcacc ggtacgctgg gcggcaccat gacccgctcc
     7921 tggccgccgg gaaataccct ggtcgcgcgg ctgatgaact gctgcggcgg ctatctgaac
     7981 cattacggcg actactcctc cgcgcaaatt gcggaaggtt tgaactatac ctacggcggc
     8041 tgggcagatg gcaacagccc gtcggatatc gaaaacagta agctggtagt gctgtttggt
     8101 aataaccctg gcgaaacgcg aatgagtggc ggtggggtga cttactatct tgaacaggca
     8161 cgccagaaat ctaatgcccg catgatcatc atcgatccgc gctataccga caccggtgcc
     8221 gggcgcgaag atgagtggat ccctattcgt ccgggaacag atgccgcact ggttaacggt
     8281 ctggcgtacg tcatgatcac tgaaaacctg gtggatcagg cattcctcga taaatattgc
     8341 gttggctacg atgagaaaac cctgccagcc agtgcgccga aaaatggcca ctataaagct
     8401 tatattctgg gtgaagggcc agatggcgtg gctaaaacac cggaatgggc ctcacaaatc
     8461 accggtgttc cggcagacaa aatcatcaaa ctggctcgtg aaatcggcag taccaaaccg
     8521 gcgtttatca gccagggatg gggcccgcag cgtcacgcta acggtgaaat cgcaacccgt
     8581 gctatctcga tgctggcgat tctgaccggt aacgttggta ttaacggagg caacagcggc
     8641 gcgcgtgaag gttcatacag cttaccgttt gtccgtatgc cgaccttgga aaacccgatc
     8701 cagaccagca tttcgatgtt tatgtggacc gatgccattg aacgtggccc ggaaatgaca
     8761 gcgctgcgtg atggtgtacg cgggaaagat aagctggatg tgccgatcaa aatgatctgg
     8821 aactatgccg gtaactgcct gattaaccag cattctgaaa tcaaccgtac ccatgaaatc
     8881 cttcaggatg ataagaagtg cgagctgatt gtggttatcg actgccacat gacctcatcg
     8941 gcgaaatatg ctgacatcct gctgcctgac tgcaccgctt ccgaacagat ggactttgca
     9001 ctggatgcat cctgcgggaa tatgtcttac gtgattttca acgatcaggt gattaaaccg
     9061 cgctttgaat gtaagaccat ctatgaaatg accagcgaac tggcaaaacg tcttggcgtt
     9121 gagcaacagt ttactgaagg ccgtacacag gaagagtgga tgcggcatct gtatgcccag
     9181 tcgcgggaag cgattcctga actgccaacg tttgaagagt tccgcaagca ggggatcttt
     9241 aaaaagcgcg acccacaagg gcaccacgtt gcttataaag ctttccgtga agatcctcag
     9301 gcaaatccac tgaccacgcc atcgggcaaa attgagattt attcgcaggc gctggctgac
     9361 attgccgcta cctgggaatt gccagaaggc gatgtaatcg atccactgcc gatctacacg
     9421 ccgggctttg aaagttatca ggatccgttg aacaaacagt atccgctgca gcttacgggt
     9481 ttccactata aatctcgcgt tcactcaact tacggcaacg ttgatgtgct gaaagcagct
     9541 tgccgtcagg aaatgtggat caacccgctt gatgcgcaaa aacgcggtat caacaacggc
     9601 gataaagtca ggatctttaa cgatcgtggt gaggttcata ttgaggcgaa agtgacgcca
     9661 cgaatgatgc cgggtgtggt cgcactgggt gaaggtgcct ggtatgaccc ggatacaaaa
     9721 cgtgtcgata agggcggttg tatcaacgta ctgaccactc aacgtccgtc tcctctcgct
     9781 aaggggaatc cgtcacatac aaaccttgtt caggttgaaa aggtgtaagg agtaaccgat
     9841 gacaacccag tatggatttt ttattgattc cagccgttgc accggttgca aaacctgcga
     9901 gctggcctgt aaagactaca aagatttgac gccagaagtc agcttccgcc gcatttatga
     9961 atatgctggc ggcgactggc aggaagataa cggtgtctgg caccagaacg tgtttgccta
    10021 ctatctgtcg atttcatgta accactgcga agatccggct tgtactaaag tctgcccgag
    10081 cggtgcgatg cataaacgtg aagatggttt tgttgtggtc gatgaagatg tgtgcattgg
    10141 ctgccgctac tgccatatgg cttgcccgta tggcgcaccg caatataacg aaacgaaagg
    10201 ccatatgacc aaatgcgatg gttgttatga ccgtgttgcg gagggtaaaa agccgatctg
    10261 tgttgaatct tgtccgctgc gggcgctgga tttcggccct atcgacgagc tgcgtaaaaa
    10321 acatggcgat ctggcggccg ttgcgccgtt gccgcgagct cactttacca aaccgaatat
    10381 tgtgatcaaa cccaatgcca atagccgccc gaccggggat accactggct atctggcaaa
    10441 cccgaaggag gtgtgagatg ggaagtggat ggcatgaatg gccgctgatg atcttcacgg
    10501 tcttcgggca atgtgtagca ggtggtttta tcgttctggc tttggcgctg ctcaaaggcg
    10561 acctgcgagc agaagcccag cagcgtgtta tcgcctgcat gtttggttta tgggtgctga
    10621 tgggcattgg ctttatcgcc tctatgctcc atcttggttc accaatgcgc gcttttaact
    10681 cgctcaaccg ggtaggggct tcagcactca gtaacgaaat cgccagcggt tcgatctttt
    10741 ttgccgtagg cggcatcggc tggttgctgg caatgctgaa aaagctgtca ccggcattgc
    10801 gtacgctgtg gctgatagtg acgatggttc ttggcgtcat ctttgtctgg atgatggtgc
    10861 gtgtgtataa cagcattgat accgttccga cctggtacag catctggacg ccgatgggct
    10921 tcttcctgac gatgtttatg ggcggcccgt tactgggtta cctgttgttg agtctggctg
    10981 gcgtcgatgg ctgggcgatg cgtctgctgc cagcaatttc tgtactggca ctggtagtga
    11041 gtggcgtggt gtcagtgatg cagggcgcag agctggcgac tattcatagt tctgtgcagc
    11101 aggccgcagc gctggtgccg gactatggtg cgctgatgtc ctggcggatc gtgcttttgg
    11161 ccgttgccct gtgcttgtgg attgcaccac agctaaaagg ttatcagcct gcggttccgc
    11221 tactatccgt ctcattcatt ctgcttctgg caggggaatt aatcggtcgc ggcgtattct
    11281 atggtttgca tatgaccgtg gggatggccg tcgcaagcta atcagtaaca accggggttt
    11341 cggccccggt tctcttttat ttctgcttcg ttaacgtgtc ataactggtc atcaaattac
    11401 gataatcagg aatgtggtta gagaacaacg tcgccagtcc ttcaatatca ttacgccagt
    11461 cgcgatgcag ctcgcacgcc acgccgaacc atgtcatcaa ttgcgcacca gcttgcgaca
    11521 tgcgatccca tgccgaatgc cgggtaattt cattaaaggt ccctgacgcg tcagtcacga
    11581 caaagacgtc aaacccttct tcaatggctg aaagcgccgg gaatgccacg caaacttcgg
    11641 ttaccacacc ggcaataatt aactgttttt tacctgtcgc tttgacagct tttacaaaat
    11701 cttcgttatc ccaggcgtta atatttcccg ggcgagcaat gtaaggtgta tcggggaatt
    11761 gtgctttcag ttctggaact aatgggccgt taggaccggt ttcaaaactg gtggtaagaa
    11821 tagttggtaa gttgaaatac ttagccaggt cacccagcgc cagcacgtta tttttaaact
    11881 tatcaggttc gatatcccgt acaagggaaa gtaaaccagc ctggtgatca acaagcaaaa
    11941 cggcagcatc atttttatca agacgaacat acggtttggt catcctcttc tcctttcgag
    12001 agtcagtggt acgtctgaaa tcgatcagac gcgatgcatt gctctgaaag catagacggg
    12061 aaatatgagt ttgctgtgac catgaaattt ttcgactgaa cgaagcgtat gaaatttgtg
    12121 ttagttcaat aaaaacaatc agatgagact aatcatctct tcagcattag cgttatgaat
    12181 gagtaatata aatcgataat accgccgctg ggatatcgcg tattttcacc cattgacaat
    12241 gtttttggcg gtggcatgat gcgcatgaaa tttgaacttc ctcacggttt taattcatgt
    12301 ccacgtatac ccggcctgtc atgcttttgc tgtctggcct gcttttgttg actctggcga
    12361 ttgcggtgtt aaatacactc gtgccgcttt ggctcgccca ggaacacatg tccacatggc
    12421 aggtaggcgt tgtcagctca tcctatttta ccggcaacct tgtcggtaca ttgctgacag
    12481 ggtatgtcat taagcgcatt ggctttaacc gcagctatta tctggcctcc ttcatttttg
    12541 ccgctggctg tgccggcctt ggcctgatga ttggattctg gagctggttg gcttggcgtt
    12601 ttgtcgcggg cgtcggctgt gccatgattt gggtggttgt tgagagcgcg ctgatgtgca
    12661 gtgggacgtc acgtaaccgt gggcgtttgc ttgctgcgta tatgatggtt tattacgtgg
    12721 gaacgttttt aggccagtta ctggtcagca aagtttcaac cgagctgatg tccgtattgc
    12781 cgtgggttac aggtttgacg ttggcaggga gcttaccgct gttgtttacg cgtgtgctga
    12841 atcagcaggc tgaaaaccat gattcgacgt caattacgtc aatgctaaaa ctccgtcagg
    12901 cgcggcttgg cgtgaatggc tgcattatct caggaatcgt tctgggatct ctatatggcc
    12961 tgatgccgct gtacctcaat cacaaagggg tgagcaatgc cagcattggt ttctggatgg
    13021 cggtactggt cagtgcgggt atccttggac aatggccgat tggacgtctg gcggataagt
    13081 ttggtcgact gctggtgttg cgtgttcagg tctttgtcgt cattctcggc agtatcgcga
    13141 tgcttagcca ggcggcgatg gccccagcgt tattcatcct cggtgccgct ggctttacgc
    13201 tatatccggt ggcgatggca tgggcttgcg agaaagttga acatcatcaa ctggtggcga
    13261 tgaaccaggc cttactgttg agctatactg tgggaagtct gcttggcccg tcatttaccg
    13321 ctatgctaat gcagaatttc tccgataatt tattgtttat catgatcgcc agcgtatcgt
    13381 ttatctattt gctgatgctg ctgcgcaacg ccggtcatac gccgaaaccc gttgctcacg
    13441 tgtaaatgaa ttcaagcaga gtgtgaactt actgtttcac actctgcttt tttgtttctt
    13501 ctatctgact tgctttattc caaattttat tcgtttaaaa ataaaatgtg cagcaggtta
    13561 taattttgca tttcgctatt tccgcacttc ttatttgccg cgcataatcc ctcgttttac
    13621 agatgcccct ttaattttgg cgaaggattt gtctatggct gggaatgttc aggaaaaaca
    13681 gctgcgatgg tacaacattg cgctgatgtc ttttatcact gtctggggtt ttggcaacgt
    13741 tgttaataac tacgccaacc aggggctggt ggttgttttt tcatgggtgt ttatctttgc
    13801 actctatttc acaccttatg cgctaattgt tggtcagtta ggctcgacct tcaaagatgg
    13861 gaagggcggg gtcagtacct ggattaaaca cacgatggga cccggactgg cttatctcgc
    13921 cgcgtggacc tactgggtgg tgcatattcc ctatctggca caaaaacccc aggcaattct
    13981 gattgcgctc ggttgggcga tgaaaggcga cggttcgcta atcaaagaat attcagtcgt
    14041 agcgttacag gggttaacgc tggtgctgtt tatcttcttt atgtgggttg cttcacgcgg
    14101 tatgaaatcg ctgaaaatcg tcggttctgt ggcagggatt gcgatgtttg ttatgtcgct
    14161 cctgtatgtg gcgatggcgg taaccgcgcc tgcgattact gaagtgcata ttgcgaccac
    14221 aaacattacc tgggaaacgt tcattcctca tatcgacttt acctacatta ccactatttc
    14281 aatgctggtt ttcgcggttg gcggagcaga gaagatttct ccttacgtta atcaaacgcg
    14341 caacccagga aaagaatttc caaaagggat gctatgcctg gcggtgatgg ttgcggtttg
    14401 tgccattctg ggctcactgg cgatggggat gatgtttgac tcgcgtaata tcccggatga
    14461 cttaatgact aacggtcaat attacgcctt tcagaagctg ggcgagtatt acaacatggg
    14521 taatacttta atggtgattt acgccattgc gaataccctg ggacaagtag ctgcgctggt
    14581 attctcgatt gatgccccgc ttaaagtgct attaggtgat gctgacagca aatatattcc
    14641 agccagttta tgtcgtacca acgcttctgg tacgcccgtt aatggctatt ttctgaccct
    14701 ggtgctggtg gcaattctga ttatgctgcc gactctcggc atcggtgata tgaacaatct
    14761 ctataaatgg ctgttgaatc ttaactcggt agtgatgccg ctgcgttatt tatgggtatt
    14821 tgttgcattt attgcagtcg ttcgcctggc gcagaaatat aaaccagaat atgtctttat
    14881 tcgtaataag cctctggcaa tgaccgtcgg gatttggtgt tttgccttta ccgcctttgc
    14941 ctgtttgacg gggatcttcc cgaaaatgga agccttcact gcagagtgga ccttccagtt
    15001 ggcgctgaat gttgcaacgc cgtttgtgct ggtaggatta ggactgattt tcccgctgca
    15061 ggcgcgtaaa gcgaatagta aataattatt gtggtcggtg acgctgactc tggcagatct
    15121 gacccgtttt tacataatcg agaaatgcgc gcaagccgca ggacatatac tggcggtttg
    15181 gataatagat ctggaagcct ggacgctctg tgctccagtc tcccagtaca cattctaaac
    15241 gaccagtatc aaggtactcc ttgatctgtt catagagcag atacccgata ccagcaccca
    15301 tcaaaacggc ctccagttca gcatcaacat catccagaat gatatttcct gcgacagcaa
    15361 tttccagctc ctttgcaaat tgccagtgga aaggtttgcc gctgggataa cgaaaaacaa
    15421 cacattgatg attaagcaga tcatgaggat ggcggggttt gccatatctt gcaaagtact
    15481 ctggcgttgc tgctacacat aatttcactg gtgggccgat cgccacgctg atcatatctt
    15541 tttcgacgat gccactaagt cgaacgccag cgtcaaagcc ctgttggacg atatcgacaa
    15601 gagaatcgtc agttgtgagc tcgactttaa tatcaggata ttcacgtgtg aaaccaacca
    15661 gtaaaggcat taaaaatata cgtgcagcta cccgggcggc attgattttg agcgttcctg
    15721 tcggtgtcag acgaaaatca ttcatctcat ctaacatgat ctgaatttcg tcgaaagctg
    15781 ggcgcaatcg ttcatagaga ttagaacccg cttccgttaa cgatacgctt cgggttgtcc
    15841 gattgaataa gcgaatttta agacgttgtt ccagtgtttt aatactatgg ctaatggcgg
    15901 acgaggataa gcctaattca tcgcccgctg cacgaaagct ttgattacgg gccacggcaa
    15961 agaaagtggc aaagtcagac atattcatcc gcattagtga aaatccttca ttaactcatc
    16021 ctgaatcatc acgtttacag gattatgccg cctgcgtaaa gtgcgctcca gaacttaacg
    16081 tggaggtaaa attatgcagt ctgaacgtat ttatttggta tgggcccatc ctcgtcatga
    16141 ttcattgacc gcacatattg ctgatgcgat ccatcagcgg gcaatggagc ggaaaataca
    16201 ggtgacggaa ctcgatttat atcggcgtaa tttcaacccg gtgatgacgt cggaagatga
    16261 accagactgg aagaatatgg ataaacgtta ttctccagag gttcatcagc tttattcaga
    16321 gctgcttgag catgacacgt tagtggtggt ttttcctctc tggtggtaca gcttcccggc
    16381 aatgctaaaa ggatatattg acagagtatg gaataatggg ctggcttatg gagatgggca
    16441 caaattacca ttcaataaag ttcgttgggt ggcgctggtt ggaggagaca aagaatcatt
    16501 tgtccagatg ggctgggaaa aaaatataag cgattattta aaaaatatgt gcagttatct
    16561 tggtattgaa gatgccgatg tcactttctt gtgtaataca gtggtattcg atggggaaga
    16621 acttcacgcg agctattatc agtcgttatt atctcaggta cgggatatgg tagatgcact
    16681 ataagatgtg ttaaaaacgc tgtagcagaa tgaagcgcgg aataaaaaag cggcaactca
    16741 ataaagttgc cgctttacgg ggaaattaga acattacctt atgaccgtac tgctcaagaa
    16801 tgcctttcac gcgttccatg gtctctttct tcggtggttt aacaccgtcg agtttgtact
    16861 cttcacccat tgccacccat ttgtgtttgc ccagctcgtg gtaggggaga agctcgattt
    16921 tctcaacgtt gcccatatca cgggtaaatt caccgaggcg atgcgctgaa tcgtcatcgt
    16981 cagaccagcc tgggacaaca acgtagcgga tccacacctt cacatttttg ttcgccagat
    17041 atttggcgaa ctccagcgtg cggtgattgg aaactccaac cagattttgg tggatctcgt
    17101 cgttcatctg tttgagatcg agcattacca ggtcggttac ttccagcagt tcatcaatca
    17161 ccggatcgta acgacgaaca aaaccgttgg tgtccagaca ggtatgaatg ccttcttttt
    17221 tgcaggcgcg gaaccagtca cgaacaaact cagcttgtag gattgcctca ccgccggatg
    17281 cggtaacgcc gccgccggaa gcgttcataa agtggcgata ggtcaccacc tctttcatta
    17341 aatcttcaac ggtaacttct ttaccgccat gcgtatccca ggtgtcgcgg ttatgacaat
    17401 acaggcagcg catcaggcag ccctggaaaa aggtgataaa gcgaatacct gggccgtcta
    17461 cggttccaca ggattcaaag gagtgaatgc gaccaataac tgacattgcg gtgtttctcc
    17521 agatgtggcc catctgaggc cgtgttggtg cgcagctcga aggctacgtc gagtctgttt
    17581 tggcagtcac cttaaagtat agatagctga caaaaaaggc tctcgtgcta aaaaaggccc
    17641 cactttcgtg gagcctttat tgtacgcttt ttactgtacg atttcagtca aatctaatta
    17701 catagattga gtgaaggtac gagtaataac gtcctgctgc tgttctttag tcagcgagtt
    17761 gaaacgtact gcgtagccag atacacggat ggtcagctgc ggatattttt ccgggttttc
    17821 catcgcgtcg agcagcattt cacggttcat cacgttaacg ttcaggtgct gaccaccttc
    17881 gatggatgct tcgtggtgga agtaaccatc catcagacca gccaggttgg tcttacgaac
    17941 ttcgtcgtct ttacccagtg cgttcggaac gatagagaag gtgtaggaga taccatcttt
    18001 agcgtaagca aacggcagtt tagcaacgga agtcagagac gctacagcac ctttctggtc
    18061 acgaccgtgc atcgggttag cacccggtcc gaacggcgcg ccagcacgac gaccgtctgg
    18121 ggtgttacca gttttcttac catacacaac gttagaagtg atggtcagaa cagactgagt
    18181 cgggatagcg tcacggtagg tgtgcagttt ctgaattttc ttcatgaaac gttctaccag
    18241 gtcaacagcc aggtcatcta cacgcggatc attgttacca aactgcgggt attcgccttc
    18301 gatttcgaag tcgatagcca gaccgtcttc gtcacgaatc ggtttaactt tcgcatattt
    18361 gattgcagac agggagtcag cagcaacgga cagaccagcg ataccacacg ccatagtgcg
    18421 gataacgtca cggtcgtgca gcgccatcag agaggcttcg tagctgtact tgtcgtgcat
    18481 gtagtggatg atgttcagtg cagtgatgta ctgtttagcc agccagtcca tgaagtgatc
    18541 catgcgctcc atcacttcat catagttcag gacatcgcct ttgatcggtt cagacttcgg
    18601 accaacctgc attttcagtt tttcgtcaac gccgccgttg attgcgtaca gcatggtttt
    18661 cgccaggttt gcacgcgcac cgaagaactg catttgttta ccaacgatca tcgggcttac
    18721 gcagcaagca atagcgtagt catcgttgtt gaagtccgga cgcatcaggt catcgttctc
    18781 atactgcaga gaagaggtgt cgatggacac tttagcggcg aatttcttga agttcagcgg
    18841 cagtttttca gaccacagaa tggtcatgtt cggttccgga gacggaccca tggtgtacag
    18901 ggtgttcagg aaacggaagc tgtttttggt aaccagggta cgaccgtcga ggcccatacc
    18961 accgatagat tcggttgccc agatcgggtc gccagagaac agttcatcgt actccggagt
    19021 acgcaggaag cgaaccatac gcagtttcat gaccaggtgg tcaaccattt cctgcgcttc
    19081 ttgttcggtg atcttgccag ctttcaggtc acgttcgatg tacacatcca ggaaggtgga
    19141 ggtacgaccg aaggacattg cagcaccgtt ctgagactta acagcagcca ggtagccgaa
    19201 gtaagtccac tggatagctt cctgagcgtt ggtagccgga ccagagatgt cgtagccgta
    19261 tttcgcagcc atttctttca tctgacccag agcgcggtgc tgttcagcga tttcttcgcg
    19321 cagacggata gtctgttcca ggtttacgcc gttttccaga tcagcctgca gagaagtgaa
    19381 ctgtgccagt ttgtctttca tcaggtagtc gataccgtac agcgcaacgc gacggtagtc
    19441 accgatgata cggccacggc catatgcatc tggcagaccg gtcagaacac cagatttacg
    19501 gcaacgcagg atgtccggag tgtaaacgtc gaacacgccc tggttgtgag ttttacggta
    19561 ttcagtgaag atttttttga tcatcggatc cagttcgcgg ttgtacgctt tgcaggaacc
    19621 ttcgatcatt ttgataccac cgaacgggat aagagcacgt ttcagcggag cttcagtctg
    19681 cagaccaacg attttctcaa gctgcttgtt gatgtagcca gcgtcgtgag aggtgatggt
    19741 ggaagcaaca gcggtgtcaa agtcaactgg cgcgtgagtg cggttttcca gtttaacgcc
    19801 ttccattact ttgtcccaca gggtggtggt cgcttcagta gcgccagcca ggaaggactc
    19861 gtcaccctcg tacggagtgt agtttttctg aatgaagtca cggacgttta cttcattctg
    19921 ccagtcacct ttggtaaaac cttcccaggc tgtggctaac ttttcattaa gctcggacat
    19981 gtaacaccta ccttcttaag tggatttttt atttactgcg tacttcgaca accattaatg
    20041 gtggtcgttt tcacgcaggt aaatgaccca gtatgtcaac ccaaccaaca aaccaccacc
    20101 gataatgttg ccgatcgtaa ccggaatcag gttatcagtg atgaaattca tcacggtcag
    20161 gtgagaaaaa ttttccggtg cagaaccgac tgcggtccaa aattccgggg atgcgaagtc
    20221 gcggattaca atacccatcg ggatcataaa catgtttgcg atactgtgct caaaaccgct
    20281 ggcaacaaac atcgcgaccg gcagcaccat aatgaacgct ttgtccatca ggctgcggcc
    20341 agaataactc atccatactg ccagacatac catcaggttt gccaggatac caagacagac
    20401 ggcctcaata aaagtatggt gcactttgtg gtcggcggtt tgtaggacgt ttagtcccca
    20461 ttgaccattt gcggtcatat actcgccgga aagccacatt aaaagtacaa acagcagtgc
    20521 gccgaccagg ttgccaaaat agacatttag ccagtttttc gccaactgac cccaggtgat
    20581 gcgcccactc gccttagcaa caacaatcaa cacggtggaa gtaaagagat cggctccgca
    20641 gacaacacaa agaatcagcc ccagagagaa gcaaatgccg ccaaccagtt ttgccatgcc
    20701 gaagggcatt gtgcctgtgc cagtggttgc tgtgatatag aagacgaatg cgattgagat
    20761 gaaaacaccg gcggtaatcg ccagatagaa agtcttaagc ggatgtttcg ttgctttata
    20821 gacacccgcc tcttcggcca ctttggccat tgcagcagga agtaaaagat caaaagggtt
    20881 gtcagctttc acactaactc tctctttatt aagtcggcga cgagatacta acaaagcatt
    20941 atagatgaga aattgatata gatcatatct cgcctggctt ataggcccgt aactcgcatg
    21001 gtttttatgc aaatacggag taaatatttg attatccaaa taaaaataaa ttttaaaaat
    21061 taacaaatga gttgaatttt ttccgcatcc tccgctaaaa cagttaatta aaagggagca
    21121 tcaggcga
//
LOCUS       DAZGXZ010000086        21118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000086 DAZGXZ010000000
VERSION     DAZGXZ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.086"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            3..110
                     /locus_tag="ACHKJF_003333"
     CDS             3..110
                     /locus_tag="ACHKJF_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0950642.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(497..1081)
                     /locus_tag="ACHKJF_003334"
     CDS             complement(497..1081)
                     /locus_tag="ACHKJF_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950643.1"
                     /translation="MNMKNSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINVQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEREHVSMEWLVNGYGDEPSMKKEPIEQTKRTKKQ
                     RIDDSDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTVLLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKAGA"
     gene            1249..1497
                     /locus_tag="ACHKJF_003335"
     CDS             1249..1497
                     /locus_tag="ACHKJF_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0950644.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            1499..3589
                     /locus_tag="ACHKJF_003336"
     CDS             1499..3589
                     /locus_tag="ACHKJF_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0950645.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSSDVVDAVEAYRGSPQ
                     LMVERLNALTENQRQVADARIAIVSEVMKVAQQPGFSCAKAIRFIVDNLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSKMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADILDKEVVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFTALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPVKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            3661..4593
                     /locus_tag="ACHKJF_003337"
     CDS             3661..4593
                     /locus_tag="ACHKJF_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0950646.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            4596..4817
                     /locus_tag="ACHKJF_003338"
     CDS             4596..4817
                     /locus_tag="ACHKJF_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950647.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATDVYSGICRSVGAKQVS"
     gene            4830..5084
                     /locus_tag="ACHKJF_003339"
     CDS             4830..5084
                     /locus_tag="ACHKJF_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950648.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            5086..5367
                     /locus_tag="ACHKJF_003340"
     CDS             5086..5367
                     /locus_tag="ACHKJF_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFP0950649.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            5364..5636
                     /locus_tag="ACHKJF_003341"
     CDS             5364..5636
                     /locus_tag="ACHKJF_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950650.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFNQQG"
     gene            5641..5934
                     /locus_tag="ACHKJF_003342"
     CDS             5641..5934
                     /locus_tag="ACHKJF_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950651.1"
                     /translation="MSKVRVIFEFKHVSHDEKPAGNDCVEVHEKIGVDVKTERDTNNR
                     PTSLCDVYASILQYHSPEIIQFLSAEFQASVQAFGADAIIKRHRVHKASGTLQ"
     gene            5946..6476
                     /locus_tag="ACHKJF_003343"
     CDS             5946..6476
                     /locus_tag="ACHKJF_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0950652.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGNPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            6574..7116
                     /locus_tag="ACHKJF_003344"
     CDS             6574..7116
                     /locus_tag="ACHKJF_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950653.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGALPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVALTEE"
     gene            7120..7653
                     /locus_tag="ACHKJF_003345"
     CDS             7120..7653
                     /locus_tag="ACHKJF_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950654.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITSRVLRY
                     LIVDRTAAPAGISAEVA"
     gene            7653..8168
                     /locus_tag="ACHKJF_003346"
     CDS             7653..8168
                     /locus_tag="ACHKJF_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950655.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFCFKGLRFMDDKYPFHRDLWRKPTASNGWSCTPRTSRIPKALRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSD
                     EFYAAEKRVRG"
     gene            8172..8723
                     /locus_tag="ACHKJF_003347"
     CDS             8172..8723
                     /locus_tag="ACHKJF_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFP0950656.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKDIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAEDAVLRELTP"
     gene            8720..8905
                     /locus_tag="ACHKJF_003348"
     CDS             8720..8905
                     /locus_tag="ACHKJF_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950657.1"
                     /translation="MIKRLVFFAIFVPVLGVGFIFAITGNLSMMPDIWFFIRMSLFLF
                     IMNLLIDIYIRITGKHK"
     gene            8944..9276
                     /locus_tag="ACHKJF_003349"
     CDS             8944..9276
                     /locus_tag="ACHKJF_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950658.1"
                     /translation="MSIELRSSYEYRKFLIAGGMKPEDVEKIVSFMDKECDKRDVSEI
                     IMDDMILDSVVALSPLWIVHSLAEIAKGTDKQAAVAALQTLNEMRISPRPTLIQMILS
                     SMEDKANE"
     gene            9269..9466
                     /locus_tag="ACHKJF_003350"
     CDS             9269..9466
                     /locus_tag="ACHKJF_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950659.1"
                     /translation="MNSLPAGWARPLMAKKHHFFKTGDNISICRRWLYLAHNREPDTF
                     ESPDDCAECRRRLNKEKDNGQ"
     gene            9456..9752
                     /locus_tag="ACHKJF_003351"
     CDS             9456..9752
                     /locus_tag="ACHKJF_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950660.1"
                     /translation="MDNSSLLFWCLYITSFFGAFVITRWLCRKIICFFDKRHPVERAA
                     DALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHARWDEAEKAFHVRKKVNTV"
     gene            9749..10258
                     /locus_tag="ACHKJF_003352"
     CDS             9749..10258
                     /locus_tag="ACHKJF_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFP0950661.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKTSCRDMSPDEL
                     SRVLDVFKKRGFKVRQNPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVK
                     SQTAAQNGGEGVANWQWLEQHPALASDVLERLKRWHRRKMLAAMGMPERTLMGYDAVC
                     RQYEKSLPR"
     gene            10328..10753
                     /locus_tag="ACHKJF_003353"
     CDS             10328..10753
                     /locus_tag="ACHKJF_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP0950662.1"
                     /translation="MAETQMSMFGGDSEQLHALIDRLDDIPDDVLKKNWPRTLSELVE
                     VTGAELQRQGIEPVLAGKLARKVAAAQAAYMGGRGYYLPVGESLFAELRNNEIFSRWD
                     RGEKIESLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            10825..11325
                     /locus_tag="ACHKJF_003354"
     CDS             10825..11325
                     /locus_tag="ACHKJF_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0950663.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            11360..11788
                     /locus_tag="ACHKJF_003355"
     CDS             11360..11788
                     /locus_tag="ACHKJF_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950664.1"
                     /translation="MCGTAVTGCPVAAVNNALPALIAGACMAALGIIAVLVAAIWGMH
                     QKTQRLEDNNQVLVRERDEARQVLANQQHTLQLISQISEAATNEKQQNIQHSEGQQSV
                     VRRSLAAVPAASAPVPDDVADRVRRAVCEIRACEAGADPR"
     gene            11772..11990
                     /locus_tag="ACHKJF_003356"
     CDS             11772..11990
                     /locus_tag="ACHKJF_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950665.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            12001..12228
                     /locus_tag="ACHKJF_003357"
     CDS             12001..12228
                     /locus_tag="ACHKJF_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0950666.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKRRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            12209..12517
                     /locus_tag="ACHKJF_003358"
     CDS             12209..12517
                     /locus_tag="ACHKJF_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFP0950667.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            12514..12804
                     /locus_tag="ACHKJF_003359"
     CDS             12514..12804
                     /locus_tag="ACHKJF_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP0950668.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            12807..13388
                     /locus_tag="ACHKJF_003360"
     CDS             12807..13388
                     /locus_tag="ACHKJF_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFP0950669.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQLESRIKGILLGKA"
     gene            13388..15052
                     /locus_tag="ACHKJF_003361"
     CDS             13388..15052
                     /locus_tag="ACHKJF_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950670.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMLRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARIGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAVNARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            15052..16641
                     /locus_tag="ACHKJF_003362"
     CDS             15052..16641
                     /locus_tag="ACHKJF_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFP0950671.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSAALDIPQIASRYIEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKIGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            16625..17956
                     /locus_tag="ACHKJF_003363"
     CDS             16625..17956
                     /locus_tag="ACHKJF_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFP0950672.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGAHISWNWFETDADVHARS
                     FTAARVARLDVLTTLQAEVQRAVDEGISQKAFISTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSMKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGDID"
     gene            complement(17958..18037)
                     /locus_tag="ACHKJF_003364"
     tRNA            complement(17958..18037)
                     /locus_tag="ACHKJF_003364"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            18078..18551
                     /locus_tag="ACHKJF_003365"
     CDS             18078..18551
                     /locus_tag="ACHKJF_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0950673.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLTRSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            18728..19851
                     /locus_tag="ACHKJF_003366"
                     /pseudo
     CDS             18728..19851
                     /locus_tag="ACHKJF_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
     gene            19851..20798
                     /locus_tag="ACHKJF_003367"
     CDS             19851..20798
                     /locus_tag="ACHKJF_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFP0950674.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAVQPDELVFAALRDGISAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            20842..>21118
                     /locus_tag="ACHKJF_003368"
     CDS             20842..>21118
                     /locus_tag="ACHKJF_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFP0950675.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTSADDAGSVAGVVHAVACTD"
BASE COUNT         5110 a         5213 c         6007 g         4788 t
ORIGIN      
        1 atatgacgct cgcgaagttt gccatgattt tctggcacga cctggcagca ccgatcctgg
       61 cggggattat taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggtgaaaacg
      121 ggcgtcaggc tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg taacagcctg
      181 cggccatttt tgtctttatc tcttccacaa gtaaatattg gcaactgtaa ccaggtgaat
      241 catttgatac caacgagggt tttagtgaaa ggaggtgaaa tcacctgtaa gatactgaaa
      301 taaaacaggt gatgtctgga tgagcgctta aagtctattt gataccgatt gtgcaataat
      361 taatcgttta ttctaaaatc ggcaaaactt ttgatactac ggattgacgc taaaatggct
      421 ttaaaaaggc ttaaaacgtt tttctccaga ttgtctttaa aggagtgtac tacagcacaa
      481 aaattgcacc actcccttac gcaccagcct ttttctgggt tacatttaaa tgattaacct
      541 tagcttcaca ttcttcataa atctctctta cagtatcatc gggtaactct tgtagactca
      601 atgctagtgc tcttttgact ccgctcaatg agtgtagttt ttgaatatct gtatcaagca
      661 aaacggttag tagttcggcc ccttttctga tcaataagtt ggacaaaatc tcttgttcag
      721 atgaagttag cgagtcccac gccctcatga tgatggaatc actatcatca attctttgtt
      781 tttttgttct ttttgtttgt tcaattggtt cttttttcat gcttggctca tcaccataac
      841 cattgactag ccactccata gatacatgtt ctctttctgc aatgttctgg agatggtgct
      901 ttcttggttg tggtggcggc tgattgtttc tccttttgta gtagttctgc aatgtctgaa
      961 cgtttattcc ccatgctcta gccgcctcac ttctcgattt gtaacgctct gcaagacgaa
     1021 aaatccgttc gatcactggt tcttttcctt cgtcgagaaa agaactgttt ttcatgttca
     1081 tgctctcagg ttcctttgta tttatcttat tgatattaat aacaaaaaca ccgcaaagaa
     1141 taaaaagaac aaaacacctt gatttggttc ttttgtttgt tctattattt acccgtacag
     1201 atatccgcac gaatatccgc gcgggtaact ttttagaata gaggaagtat gagtagaaga
     1261 gaagtttcag atcacgattg gccgcctgaa atcatcaagg cgcgcttaca catggctggc
     1321 ttatcactgc gctctttatc tttaaaggct ggttacagca gggattcgct gaaaagcgta
     1381 ttacgtactc cttgccgacc gtatcagcaa ataattgctg atgctcttgg ggtatcgcct
     1441 gaagaaatct ggcccagtcg ataccaggtt aagagctata tgagaaaggc gtcatgatat
     1501 gtatgtcatt gcaaaagaac tgattggcgc gcccggaatg cctgctacaa caaaaggtat
     1561 tcgccaggca ttacaacgtt acgtacaagg gaaaagctgt tgttcccgtc gtcgctcagg
     1621 ctctaaagca actgaataca gcatcgactg tttacctgaa gtgacgcagc aggcattacg
     1681 tgaacgttat gccctgcaac tgatgacgca aaaagccgat gaatcaccgg ctccggtggt
     1741 gacaaaggcc agacgctcat ctgacgtggt tgatgcggtg gaggcatatc gcggatcacc
     1801 acaactgatg gtcgaacgcc tcaatgccct gactgaaaac cagcgccagg tggctgatgc
     1861 acgaatcgcg atcgtcagcg aagtgatgaa agtcgcgcaa caacccggtt tcagctgcgc
     1921 gaaagctatc cggtttatcg ttgacaacct ggcacgttcg caactggacg agcgcattgt
     1981 ggcaatggtt gagacggcga acgccaaaaa gggaaacagc cgtgcgttga gtgaaataac
     2041 gctgaaacgc tggattgcgg cctttaacaa ggcgcagaac gccgctgaac gcctgctttt
     2101 actggcaccg ggtaagcgcc aggaaataaa agccgaagat attaactggc tgcccgaatt
     2161 tctggcgcag tatcgccagt caaacggccg accaatgacc gaggcttacg aggattttgt
     2221 cgctgaatgg cagcaccggc acgctgatga gccttatatg ctcgatatca tgccgtctta
     2281 tgacaccatt cgccgcgcaa tgaagaaact gccggaagtg gtgaaacaaa aaggccgggt
     2341 gaccggcagt gaataccgcc agcttgaggg attcacgcgc cgcgactggt caaaaatgcc
     2401 ggtgaattat gtctggattg gtgacggtca cggcatgaag ctgaaatgcg cacacccggt
     2461 tcacggtcgg ccatttgcac cggaagtgac ctttgttatc gacggtggca cgcgctttgt
     2521 ggtgggctgg agccttgacc tggctgaaaa tgttttcgcc gtagccggtg ccatacagca
     2581 cggcattcgc catcacggca aaccgtttct gtattactcg gataacggct ccggggaaac
     2641 cgccgacatc ctggataagg aggttgtggg gatactgccg cgactgggga ttaatcaccc
     2701 gaccgggatt gccggtaatc cgcagggacg aggcattatc gaacggctta accgcacatt
     2761 accgatgcgc atagcccgta aataccgcac ctatttcggg aaaggtgcag atcgcgagac
     2821 gttacgcaaa accaaccgcg atttacgctc ggcatttact gccctgcaac agggcaaacg
     2881 gctgaacgcc cggcagcagt cagcgatgcg tgatttaccg tcctggtctg aactgattga
     2941 tgccattcgt gacggtgttg agtggtacaa caaccggccg cacgatgaat taccggtgaa
     3001 gccgaacggc aagcattaca gcccggcgga gttcagaaaa aaacgcctgg cggaagagga
     3061 cacggaaatt gaatggctgt ccgatgttga gctgcgggat atgttccggc cgatggtgga
     3121 acgccctgta agacgctgtg aaatacgctg gctgaataat atttactacg cgcccgagct
     3181 gcgtgacgag catggtcgta aggtgcttat cagctatgac attcatgatg ccgaacgaat
     3241 taccgtacgt cgcctggatg gcagcgtgat ttgcgaggcg gtatgggacg gtaataaacg
     3301 cgaagccttc ccggttagcg cggaatacta caaacagcag caacgcctta aaggcatgcg
     3361 taaacgcgca gaggaaaaaa tccgtgatgc cgaggatgag gttgtcaacg tgctggagca
     3421 caagccgcag gagccctggc tggaaaacat ataccgccct gtgggtaata cggtgaccgt
     3481 tcagcaaccg gtcgctgacg atgaacctga tgaagaatac gagcgtaatt tccagcgggg
     3541 attgcagttg ctggaagcga aattaaaaga aaatgacccg ctggcctgaa ataaaaaaat
     3601 aacccgagcg gcgactcagg ttatttgatt aaacaaggta tcaaatgaga ggttaataat
     3661 atgactgata ttaacgatgt aatcaagacc attgatgaac ttattgatgg cggcgtactg
     3721 acgcagtatg ccatcgccag agaggcggga atttccgacg gcacattatc ggctttccgt
     3781 aagggaaaat ataaaggcga taacgctgct gtggctgctt ccctgcgttc ctggtatgag
     3841 aactggaata aacaaagcgc actgccggaa ccgccgcagt ttgtggaaac gcagacagtc
     3901 caggagctgc gcgcactgtt tcaggcggtt cgtctgatgg gctgtattaa cgttattgtg
     3961 ggcgtaccgg gtgtgggtaa aacggccact gcccgtaatt actgccagga gcaaccaaac
     4021 acctggatga tcaccctgtc acccgcgcac tccagcgtca cggagtgtct gctggagctg
     4081 gccgatgcgc tggggattga ttacacccgc gcgaacaaag gggcattatc ccgcgccatc
     4141 cgccgtcgcc tgatgggaac gcgtggactg gtgattgtgg atgaggcgga tcatcttggt
     4201 attgacggtc tggagcaact ccgggcaatt caggacgcca cggggatcgg gatggtgctt
     4261 attggtaacc cgcgcggatt gtttaaaggt ggacgccgcg cctttgatga tttatcgcgc
     4321 ctgttcagcc gtcttgcccg tacaaaacaa cttcgcaagg ccaaaaaggc ggatgtgctg
     4381 gccattgcca gggcgtgggg gatcagtggt gaggccgagc tggccgtcat gcaggctatc
     4441 gctgaaaagc cgggagcgtt acgcgttctg acacatacgc ttaaccaggc gtggctcacc
     4501 gccagcggtg aaggcgcggc gctgacagaa aaacatatta atgcggcctt taaagaggtt
     4561 tataccaacc ctgaattact ctcacaggtg tgattatggc ggtatttaat attcctgata
     4621 tttacggacg cttttacctg gttaatttcg ataacgtgaa ggtgatttca ctggccgaaa
     4681 ataaagaatg tggcgattta ctttttgaat ttaatgaccg cacacgaatg gtgatatctg
     4741 ccggacttga tcgcgaaggt gcgacagacg tttacagcgg aatatgccgt tctgttggtg
     4801 cgaaacaagt cagctaaatg aggtgttata tgaatatgca atcctgcggt aacaaaatga
     4861 atttattcga ctccctgaac agcgcgcgcc gtctgaccga acttgccggt gcggtactgg
     4921 aacgcagtaa gcgctatccg caacgttttg cactgaaaac cacgccgccg gtaggcaacg
     4981 tgcagggaac cggtgaaatt gaaatcacca tacagaccaa cggcctgcgc cgccgtgtga
     5041 aggccacccg catcagcggc tgcacggttt actgggaggt gtgaggtgaa aaaaaatctc
     5101 attgcatggg cgtggtcgag tggtcttatt gagtttggtt acgtcctgcc ggaaggtgca
     5161 ttgccgatag ttgccggaaa gcctgccacg gtacggcatg tgattgaggt tatggcgcgt
     5221 catggacgtg atgaacagga gcagttactg gttccgggga taccggaagc ggtgacggag
     5281 gaagaagcct ttaatgccat gattcggttc tgccgtgagg tcagacgccg ggtcagttat
     5341 ccaaacagaa cgaggaccag aggatgagta aagtcgtacg cattattttc gaatacaagg
     5401 agcacgttat ccataaaaac gctgatggaa cagtgcgcat gggggtaagt ctggacatac
     5461 gttcaaccgg gataaagcag aaaggtgatg gacccgccat gatttttggg gtggttatgc
     5521 tcgcggaaag cagagacttt gctgaacttg tggcaatgaa agccagtgcg ctcatgaaag
     5581 atatgagcat gcgttccggg gttattaaag gtaatgaatt taatcagcag gggtaattcc
     5641 atgagcaaag tacgcgttat ttttgaattt aagcatgttt cgcatgacga aaaaccggca
     5701 ggcaatgact gtgttgaagt gcatgaaaag attggtgtgg atgtaaaaac agaacgtgat
     5761 acgaataaca ggccgacgtc actctgtgac gtttatgcaa gtattctcca gtatcacagt
     5821 cctgaaatta ttcagtttct ctcagcggaa tttcaggcat ctgtacaggc ttttggggcg
     5881 gatgccatca ttaaacgcca ccgcgtgcat aaagcatcag gcacactgca ataaggaaaa
     5941 acaaaatggc taaacgtgtt acaaaattaa aggccgcagc cgaggctgca ccgcagaccc
     6001 gcgaagaagt cagccgcgat atccgtaccc tgggcgatat tcagcgagag gcgctgcggc
     6061 tggaaacggc gatgaatgat gaagtggcag aaatcaccgc ccgttatacg ccgcagattg
     6121 aaaaccttaa aaaagaaatc aaagtgcttt ttaaggggat tcaggactgg tgcaaaacca
     6181 accgcgatga gctgacgaac ggcggcaaaa ccaaaacagc caatctgacc accggaacag
     6241 tgtcatggcg gctgggaaat ccatcatgca gcgtcagccg tgatgtggaa ggtgtgattg
     6301 aaatgctgcg ccgtatgggg cttgagcgat tcatccgcac gaaagaggaa gtgaacaagg
     6361 aagcggtcct ggcggagccg gatgcagtga aaggcattgc cggtattaag gtgaataaag
     6421 gcgctgaaag tttttatgtc gagccttttg aacaggacgc cggactgaat aaataacacc
     6481 gcattaaatc tttaaatatc acatcgtttt aagtatggcg ctcgcgtcag gggactgcct
     6541 gcgcctgtaa actgaaaata aggaacagga tatatggcat atttttattt caaattagac
     6601 cgtgtgcaga caaataaata tttcaccaaa tatcaacaga ctgttttacc gctacgcaac
     6661 agtattcttc gggcattact gaaaaataca ggcgctgccg gattgcgctt aaagccgttt
     6721 gccatggacg taatcagtga gttttatttt tctggtgctc tgcctgcggg ctggcgtaag
     6781 cgcgatgatg tggcttttat cggggacgga ccgtgcttta ttgccagacc tgatgagtca
     6841 tgccctgaag gaccggcgat tgccgcaatg attgaagccg ctgaacgcga gttaagaaag
     6901 cgtcctgatt tccttgtctg gctctgtgaa aagctggggg taatgagaat cccctccatg
     6961 tttaacatgg actcctggtg gaccccgtcg ctctcccgtg atgccctgtg cgtggtgttt
     7021 aaagtaggcg cttatggcag ggaaataaaa gggggtattc ctgaagaatg ccaggagatt
     7081 aaacattctg aatatgtggc gctgacggag gaataattca tgattgatgc aaaagtgctt
     7141 gaaggggtta aaaactggct gagtatttac gggcgtctga cctgcggcat tctggctgaa
     7201 aaaatgaata tgccgccatc ctcgatggtt tattttctgc gtgatgcggt cgatgccggg
     7261 gtgctgacgg aatgtaacgg tttttatgat attccgcgtc cccgcccggt gcagccggtt
     7321 cgtcgcaaat gcagccagga aggtgcggct gatgatgttc agtggtgcag cttcagaaaa
     7381 tccctgccgt ggattgaggg gcatgatatt ccgtcaatgg cgtgggaatt tgctcagggc
     7441 gttctgacct gtgaaaccgt ttatgtggtg gctgaagttg atgagcaggc catgaaagaa
     7501 ggcgtgcccc agtttgtgat ggcgtatatc gacatacgcc tgggtgtcat tatctgcggt
     7561 ttaagcggct ggaatatcac ctcgcgcgtt ctgcgttacc tgattgttga ccggacggcc
     7621 gcgcctgccg ggatatctgc ggaggtggcg taatgttctt taaaacatca aacccttccg
     7681 cgctggccgc gtggcaaaaa tatcagcagg actgccagaa agttaaggat gaggcaaaac
     7741 gccttgaggc cgtgctgaat gttgcgtgcc ggtcggtatt tgtatccggt atcagtggct
     7801 tttgttttaa agggctgcgc tttatggatg acaaatatcc ttttcatcgc gacttatggc
     7861 gaaaaccgac tgcgtcgaat ggctggagct gcacaccgcg cacatcacgt attcccaaag
     7921 ccctgcgcgt tgcctctgac gaacttaaca gtctgtggcg tgaatattcg cccgtcacgt
     7981 atgccagaac cgatgcactg ttgttctggc tgggtattga tttctcggca atcatgtttg
     8041 gccctgtgaa gtggttctgc gttgacgatg tgatttacct tgagtgcggc gtaaaacccg
     8101 caaatcagaa aatgaccgaa attctgtctg atgagtttta tgctgctgaa aagcgagtca
     8161 gggggtgatg tatgatgatt ttacaaccca tggggcgaaa aggtcaggca cctgctcatg
     8221 tccgtgcgtg gacacctgaa gaagatgcgc tgctgattgc gctttatcca tccaccccgg
     8281 ttaaggatat tgctgtcagg gtaaaaagaa gtttctgggg tgtacataac cggattgttt
     8341 tattacgcgg tacttacccg gagttgctca aatgcaaacg tccaagattt aaacatgatg
     8401 aagataaatt tatccgaaaa aatgccagaa cgatgaccgg taagcaaata ggagaatatc
     8461 tggggcgaga ccgggattct gtccacaatc gggcgcgata tattggcgta agcatgaaaa
     8521 aatacggaga attgctgccc ttcacccgta tacctgacga tgatgttcat cttattcgtg
     8581 aattacggga tgctgaatca ccacgacgtc ttaccttccg ggaaataggc gagaaatttg
     8641 aattatccga gagtacggtg aattttattt atcaccatcg ccggactgcc gaggacgctg
     8701 tattacggga gttaacgcca tgataaagag acttgtattt ttcgcaattt tcgttccggt
     8761 gttgggggtt ggttttatat ttgcgattac aggaaacctg tccatgatgc cagatatttg
     8821 gttctttatc aggatgtcgc tcttcctgtt tattatgaat cttcttatcg acatatatat
     8881 ccgtattact ggaaaacata aatgagaaaa atcacgtcct tgtcagagtt acaggagatg
     8941 aatatgagta ttgaattaag atcgtcttat gagtatcgca aatttcttat cgccggaggc
     9001 atgaaaccgg aagatgtgga aaaaatcgtt tcttttatgg ataaggaatg tgataagcgg
     9061 gatgtgtcag aaattattat ggatgacatg attctggatt cagtcgtggc gttaagtccg
     9121 ttatggattg tgcattctct tgcggaaatt gccaaaggca ccgataaaca ggcagctgtt
     9181 gctgcccttc agacgcttaa tgaaatgcgt atatctccac gcccgacatt aatacagatg
     9241 attttgtcca gtatggagga taaagcaaat gaatagtcta cccgctggat gggcgcgccc
     9301 tctgatggct aagaagcatc atttttttaa aactggcgat aatatcagta tatgtaggcg
     9361 gtggttatat ctggctcata atcgcgagcc ggatacattt gaaagccctg atgactgtgc
     9421 cgaatgtcgc agaagactga ataaggagaa agataatgga caatagttct ctgttgttct
     9481 ggtgcttata tatcacatcc tttttcggcg catttgttat tacccggtgg ttatgtcgaa
     9541 aaatcatctg cttttttgat aaaagacatc cggttgaacg ggcggctgat gcgctcattc
     9601 agcaggccat tgtgttatat agcggtgaat ttttctgccg gataacaacc agagatggct
     9661 ggcacataat gattattcca ccaacacacc atgcccggtg ggatgaggca gaaaaggctt
     9721 tccatgtccg taaaaaggta aatacggtat gagaggaaaa ctgatatccg ccattcatgt
     9781 ggcaaagcgc gagcttgccc tggatgatga gacttataca tccgcgctgc tggcagccac
     9841 cggcaaaacc agctgccggg atatgtcacc ggatgagtta tcccgcgtgc tggatgtttt
     9901 caaaaaacgc ggttttaaag tgcgtcagaa cccggttaac cgggccttaa aaccgggtac
     9961 ggtgaccgcc aaaattcgcg ccatctggaa ggtgatgcac cggcagggct ttatctctga
    10021 tggtgcggaa accgccctta accgctgggt gaaatcgcag acggccgcgc aaaacggcgg
    10081 cgaaggtgtg gcaaactggc agtggctgga gcaacacccc gccctggcct cagatgtgct
    10141 ggagcgtctc aagcgatggc accgccgcaa aatgctggcc gcgatgggaa tgcccgaacg
    10201 cacgctgatg gggtatgacg ccgtttgcag gcagtatgaa aaatcacttc cccgttaact
    10261 ccaaatcccg ccacaacgcg ggatttttat tttaaactta ccgggaacgc gagaaccgga
    10321 ggctgatatg gcagaaactc agatgagcat gtttggtggt gacagtgagc aactgcacgc
    10381 ccttatcgac cgccttgatg acatccctga tgatgttctc aaaaagaact ggccgcggac
    10441 cctgtcagaa ctggttgaag tcaccggcgc agaacttcag cgtcagggga ttgagccggt
    10501 actggccggt aaactggcac gcaaggtggc tgcggctcag gcagcctata tgggcggacg
    10561 gggttattac ctgccggtcg gggaatctct ctttgccgag ctgcgaaaca atgagatatt
    10621 ttcgcgctgg gaccggggcg agaaaattga atcccttcgc cgccattacc ggatgtcaga
    10681 aacccagatt tataccgtca tacgcgaaca gcgccgtctg catctggcaa gaacgcaacc
    10741 gccacttttc tgatattccg caaagcctgc cgcctctcct tcaccgttac gctgactcag
    10801 agaacatcat gagacagcgt aacaatgcca aaactccccg caccactgcg taaaaagctg
    10861 attgcccttg ttctggccgg tgccgggacg ttcacgattg ccacgcatta caccggttac
    10921 tgggaaggga aagaaaactc cacgtatatc gatcccaccg gtacacccac catctgttac
    10981 ggccataccg gcccggatgt gaaaccgggt atgaccctga cagatgaaga atgcctggaa
    11041 ctactggaaa aggacatgaa atgggccttt gcggccattg atcggcgtgt tcaggtgccg
    11101 cttacccgtg gtcagacggt ggcgctggct tcgtggatct tctgggccgg tgaaacgaac
    11161 tttcgcaact ccacgcttct gcgcctgatc aatgccgggc agatgcccgc gtcctgtaag
    11221 cagtatatcc gctggattta ttcaaagggg gtgaaactcc ccggccttga ggcccgccgt
    11281 tcggcggatg aatggttatg tcgctacgac ttgccgaaag tctgaaccgc ttctggcgac
    11341 cgctcatgat agcgctgctg tgtgcggtac tgctgttacg gggtgtcctg tggctgcggt
    11401 gaataatgcc ttaccggccc tgattgccgg ggcgtgcatg gcggcgctgg ggattatcgc
    11461 tgtactggtt gccgctatat ggggaatgca ccagaagacg cagcgccttg aagacaataa
    11521 tcaggtgctt gtgcgtgagc gggatgaggc gcgtcaggtg ctggcaaatc agcagcacac
    11581 cctgcaactc atttcacaaa tcagtgaggc ggccacgaat gaaaaacaac agaacattca
    11641 gcacagcgag gggcagcaga gcgttgtccg ccggtcgctg gcggcagtgc ctgcggcctc
    11701 tgcccctgtt cctgatgatg tggctgatcg ggtgcgccgg gccgtctgtg aaatacgtgc
    11761 ctgtgaagcc ggtgccgatc cccgctgaat ggctggctga ctgcctggtc cctcctgcgc
    11821 cggagccgtt cacgtttggg gcatcggtca cttacaacct gcaactgctg gcggtgatca
    11881 agaactgcaa cgtggacaag gccagtattc gtcgtctgga ggcgcggcga caacatgaat
    11941 ttactgatat ggccggaacg cctgctgttc cggcaggaaa gacgaagtaa ggaaaaaggt
    12001 atggatgatt cagatcgtgc tcaggctgtg atggagcggg gatcagaacg cgccctttgt
    12061 aaccggctga cacgtaagcg ccgggcaatg gatgctccgg gcagacgtgt ctgcgcggac
    12121 tgtggcggag agatccccgc cgcacgtctt gttgctgtgc cggatgccat ccgttgtgtg
    12181 aactgccaga acatcatgga ggcccgtcat gtgggtcagc atcgttaaag attatgttgt
    12241 gccgatcctt tcggcgacgg caaccgctgg cgggatattc atggcgctga tgcgcaaaac
    12301 gtttgtcccc cgcgaggcct ttgaaaaact ctccgaccgc gttgagaagg tggaaacccg
    12361 cctgtcgtca ctgccgacag aagccgaggt caaccgtctg aatgtggaaa tcgtgaccct
    12421 gcggggtgaa ctgaaaacca cgaacgccac gctccgctct gtctcctatc agaacgaact
    12481 gctgctggag caggctgtaa ggaaaaaaac gcaatgagtg atttcattac tgaagatcag
    12541 cgtctggtca ttctgcggtc gctggcagat tacaacggtg aactgggtga atccgtgctc
    12601 caggactgtc tggatgatta cggccaccgc gtgtcccgcg acaccgttca cacccacatt
    12661 gcctggcttg ccgagcaggg actggtgcgt aagcgcacgc tcgttaacgg ttatttcatc
    12721 gccgaactga ccggccgggg tcaggatgtg gcggaaggtc gcgccagcgt tccaggtgtg
    12781 aagaaaccac gcgcaagggg gtaacgatgg acaagccgac gcgtggacgc gtgcgcaagg
    12841 tggatttgct ccccgacagc atccgtaagc cactgctgga aatgctgcgt gaaaaacgtc
    12901 tgacgcaggt ccagatacgc gaggaaatca accgtctgat ccgtgaggcg ggcctgccgg
    12961 aagagcagca gctttcaccg gcggccatca gccgggaagc ctcccgtaat gagctgattg
    13021 cccgtaacct gcgtgattta cgcgagcaga caaaagccat gatggccgaa ctgggagaca
    13081 agccaaccgg tgaaaccaca gcgctgattc tggagatgtc ccgcgccctg atgtaccgcc
    13141 gtctgcgtgc cgcgaccgaa tccctgaaca gtgacagcga cgtggatatg cgtcttatta
    13201 aggacatcct cctgtcagca cagcgtgcgg aaagtgccgc cgagcgcagc attaagcgtg
    13261 aaaaagaaat ccgggccgca tttgctgaag agatggcaaa cgccgtcacc gacgagctgc
    13321 gcggcgtgga cgggatgagt gaacagcttg agtcccgtat taaggggatt ctgttgggta
    13381 aagcctgatg aataacgccg aagaactctc ccccttactg acgaataccg tctccacccg
    13441 caaaattgat ctggccggtg aaaaagccct gctgggcgtg gatgtgccgg acagccttga
    13501 tttgcccggt gatatgccgg tatttctgga ttatcaggcc cgctggtttg aggatgaaag
    13561 cgaggtctgc atcgcggaaa aatcccgccg caccgggctt acctgggcgg aagccgggcg
    13621 taacgtcatt accgccgcaa agcctaagcg gcgtggtggc cgcaatgtgt tttatgtggg
    13681 gtcaaagcag gagatggcgc tggaatatat ttctgcctgc gcactgttct cccgtgcctt
    13741 taaccagctg gcagatgctg atgtgtatga gcagaccttc tgggatcggg ataaaaaaga
    13801 agaaattctg acctacatga tccgctttcc gaacagtgga ttcaaaattc aggcactgtc
    13861 ttcccgtccg tcaaacctgc gcggccttca gggggatgtg gtgattgatg aagccgcgtt
    13921 ccacgagtca ctggatgagc ttctcaaggc ggcaatggcg ctcaccatgt ggggcgcgcg
    13981 cgtgcgtatt atctccacgc ataacggcgt cgataatctg tttaatcagt acattcagga
    14041 agcccgcgaa gggcgcaagg attacagcgt ccaccgcatt accctggatg atgccattgc
    14101 ggacgggctg taccgtcgta tctgttatgt caccggccgc gaatggtcac cggaaagcga
    14161 gcagaagtgg cgtgatgatc tctacaaaaa cgccccgacc cgtgaggatg ccgacgagga
    14221 atacggctgt atcccgaaaa aatccggcgg tgcctatatt cctcacgcgc ttattgaaat
    14281 ggcgatgctc cgcgacatcc cgattctgac gtttgaagcc ccggacgact tcatcagccg
    14341 tgccgcatgg ctgcgtgaat cggaggtttt aacctggtgt gaagaacatt taaaaccgct
    14401 tttagaggcg ttaaatcccc gttcccgctt cagcttcggg gaagactttg cccgcatcgg
    14461 tgacctctcc tgctttgtgc tgctggaaat cactgaatcc ctggcaaaac gcgaggtgtt
    14521 tcgcgtggag ctgcgcaacc tgccgtatgc ccagcaggag caggtgatga tgtacatcct
    14581 gacccgcgtt ccggcgctgg tcggtgcggc gttcgacgcc accggtaacg gcggctatct
    14641 ggccgaagcc gcgctgctgg cctttggccc ggacatcatc gactgcgtga tgttatcgcc
    14701 gaagtggtac ggcgagtgga tgccaaaact gaaggccgag tttgaggatc agaatatcct
    14761 cgtcgcccgc catcagacca cgcttgatga tttgcgccat gtgaaggtgg tgaacggtat
    14821 cccgcaaatt gacaaggggc gtacgaagga ccagaacgca acggctgtga acgcccgccg
    14881 ccatggcgat tttgccgtgg cgctctgtat ggcaaaccgg gcgtcataca tggagggctt
    14941 catcctggat gaatcagcct gtcaggcgct gccggagcgg tcgcgggcca tggagggcgg
    15001 ttatcgtgat gacgatgagg catatcatga atttgatcgg gggtgctggt agtgggacgt
    15061 ataattgatc ttgacggaaa acctttctcc tttgacccgg agatgcagag cgccgcgctg
    15121 gatattccgc agattgccag ccgttatatt gaacatccgg cctcgggtat caccccgaac
    15181 cgggcggcac agtgccttcg cggggctgaa cgtggcgatc tgattgccca gtccgatctg
    15241 gccgctgaca ttgaagaaaa ggatacccac ctttttgcag agctgggcaa gcgacgcctt
    15301 gccattcagg gggtgccctg gagtattgag ccgccaccga acgccagcgc gaatgagaaa
    15361 aaggacgcgg aaatgctcga cgaatacctg cattccgccg actggtttga tgccatgctg
    15421 tttgatgcca ccgatgccat cctgaaaggc tattcctgca tggagattga gcacgggatg
    15481 ctcggtaaaa tgcacatcat ccgcgccatc cgctggcgtg acagcgggca tttctgcctt
    15541 aacccggatg atttgagcga actgcggctg cgtgacggca gccatgccgg ggtggcgttt
    15601 cagccctttg gctggatagt gcatcagtca cgttcacgca ccggttacgg cggcgcaaca
    15661 gggcttgtca gaacgcttat ctggccgttc attttcaaaa actattccgt gcgcgatctg
    15721 gctgaatttc tggaggtgta cggcctgccg atgaagatcg gtaaataccc gtccggggca
    15781 acgccggagc agaaaagcgc cctgatgcgg gcggtgatgg atatcgggcg acgtacaggc
    15841 gggatcatcc cggccgggat gtctctggag tttcaggcag ccgcgaacgg tcaggccgat
    15901 ccgtttgaaa ccatgatttc gtggggggag cgttccatct ccaaagcaat actcggcggc
    15961 acgctgacca cggaagccgg agacaaaggc gcgcgctcgc tgggtgaagt gcataacgag
    16021 gtgcgccggg aaatcaggga ttctgattta cgccagctgg ccgccacgct gaaccgcgat
    16081 ctggtgtatc cgctgtatgc cctgaacacc gcccacgcca ttgatatccg tcgcctgccg
    16141 cgtatctgtt tccagacaaa agagccgggg gatatcacca aaatcaccag tgcggtgatg
    16201 cagctcagta cggggatgga tatttctgat ccctggataa gggagcagac cggtattccg
    16261 cagcccacgc ccggtgaagc catcttccgt gtccgtcaga gtggcaatga acctgctcag
    16321 acagacaaag agatgccacc ggaaaaacag gagaagacag agcagacagc gctggctgcc
    16381 agactgccgg aagcgaaaag cagcccccgt gatgaactgg acgacatggg ggatgcggtg
    16441 cctgcccgcc ggttacagga ggccatcgac ccggtactgg agccggtcat tgatgccatc
    16501 agaacgcggg ggctggcgga ggcactggca gacctgcccg ccctttaccg tgaaatggat
    16561 gattcccgcc tgatgacgct gctcagtgat gccatgtttg ctgcggaaat gaaggggatg
    16621 ctggatggca caggggattg atttaggtta tgccgccacc cttccctcaa aagaggcggt
    16681 ggcatacttc cgcgccaaag gggcgcatat cagctggaac tggttcgaaa cagacgcgga
    16741 tgttcatgcc cgttcattca cggcggcaag agtggcacgc ctggacgtac tgaccacact
    16801 acaggcggaa gtgcagcgtg ctgttgatga ggggatttca cagaaagcat ttatcagcac
    16861 gctgacaccc cgcctgcaaa agctgggatg gtggggaaaa cagattgtgg tggacagcgc
    16921 cggtaacgca gaagaagtgc agctgggcag tccccgccgt ctggcgctga tttacaacgt
    16981 gaacacccgt gtggcttaca atgccgggcg ttacacgcag atgatgaaca acacggacac
    17041 gcatccgttc tggcagtatg tggcggtgat ggacagccgc acccgcccgg cgcattccgc
    17101 tcttaacggt ctggtattcc gctatgacga tccgttctgg aatacacact acccacccaa
    17161 tggctggaac tgtcgctgcc gtgtccggcc attgtctcag gcccgtctgg atgcaatggg
    17221 gttatccgtt tcatccggtc aggatcatct ctccacccgc aatgttgagg ctggcgtgga
    17281 taagcagacc ggggaagtca gagaaatgcc ggtgaccaca tattcagatg gcaccagaac
    17341 catgacaccg gatgtgggct ggtcatataa ccccggttcg gcggcgttcg gcacagacca
    17401 ggcgctgatc cgtaaactga tcgaggtgaa aagcccggcg ctgcgggaaa tggtggttca
    17461 ggagatgaac aacagcccgg agcggcaact ggcgttccgc atctgggcaa aaaacatcat
    17521 gaaaacccgg cgaggcggta acgatatccg cacgctgggc tttatgaccg aaagcattgc
    17581 gcaggcggtg gaaagccgga cgggaacgcc accggcccgc ctgctggcga tgagcggtaa
    17641 aaatgtgctc catgcggaca gcatgaaaca tcagaatgac ggtatcgccc tgacgccgga
    17701 ggatttcggg cgcttaccgg caatgctggc aaggccaaaa gcggtactgt gggacaaacg
    17761 gcacaacaat ctgatgtata tcgtggaaaa tcaggatggc agtgtgcaga ttgccgttaa
    17821 tgcaccgtac agcctgaagc gtcagccgga taaactggat gtgattgtta atgcttaccg
    17881 ggttatcaat atggataagc tgaaatcgga tatccggggc ggaatgcttg aggtactgga
    17941 aggagatatt gattaacggt cagtggcggg ggtcgaaccc gcatacatga tccacccgga
    18001 ggcaggcacc gactttacca gttaagcgta cactgaccgc ctgtttattt tatattcatc
    18061 ctttatgcgg aggcaatatg tcatccattg atgccgctgt cgttgttgac gttacgcgtc
    18121 tccagcgggt ctttgcccgg cttcagtttg tgggtggtgg aaaagacctg acccgcagtg
    18181 tggcgtccag ccttctgtca tcgtcagaaa tggcgtttga acaggaaaaa gagccggacg
    18241 gcgaacgctg gcatgactgg tcagatcctt accgcaagtg gcgtacccgt aagggataca
    18301 tgcccggcaa aatcctgacg ctgaacggcg atctggcccg acgcctgacc acggattatg
    18361 gcgatacctg ggcgctgatt ggatcaaatg agccttatgc ggccattcat cagtgggggg
    18421 ggctgccagg tatgccgcct ggaccggcgg ccattggtgc acgtccgtat atgggctttg
    18481 atcaggtggc agagcaggag atcatggacg aaatcagaaa acgctttaaa aaggccacag
    18541 aaacgcctta attgtttaag gtatgcaaat gcattacctt acccccttca ggcgcgtgtc
    18601 gtgattttct aacctgaatt taacgggctt taaaatctgc gtgaagcgcc tttgtatttt
    18661 tcccctgaaa gcacttcctg aaatcccgca aaacccgccc tgaaaattcc cggcttatgc
    18721 tgccggaatg aagacgaaaa acacgcccaa acttgcttat gccattctga atgccatcag
    18781 cctgtccgca gacggggacg gtgactggtg tcagatcatg ccagccggtc gggtaaaggc
    18841 ccgtgacggt cgcccggaaa aaccggccga gggctggctg atcaaccatg cagccgttga
    18901 gcgcatggtc tcccgggttg tggcgctcaa tcagccagtg aaaattgact acaaccacca
    18961 gaccctgatt aaggggcatc aggcaccggc tgccggtttt gtgatggcct caccggagaa
    19021 ttttcgcttc agtgaagagc gtggcttcga ggtgcgcccg aagtggaacc ctccggccct
    19081 tgaacatctg cgtaataacg aattcccctg gttttcaccg gtgattggct atgacgagaa
    19141 caccggcgaa cctgtcgaac ttcggatgct ggctattacc ggtgaccccg gtctgaccgg
    19201 catgaatcct gtcgccgcgc tgtcggcgga tgacctttat aacgccttaa accctccttt
    19261 aaaggatacc tccatgaatg agcaattacg ccagttgctg acggcgctcg gtctgaccgt
    19321 ggctgacggt gacgaattta cgccggagct gggcacggcg gcgctgtctg ccctcaccgg
    19381 gatcaaaacc cgtgcggatg cacacgacaa cctgaaaaca caggtcgcca gtctgtcggc
    19441 agagctggaa accgcaaaag gcacaccggc tggcggcagt attgatctga cgaaatacgt
    19501 gcccgttgaa acgtataacg ccctgcgtac cgaatacgtt gcgctgtcgg cgcagcacgg
    19561 cagcaccacg ctggagcagt tgctggacaa ggctgaatcc gaagggcgca tcttcaaaag
    19621 cgaacgcggc tatctggaac aactgggcgg gcagattggc gttgcggcac tttctgcaca
    19681 gcttgacgcc cgcagccggt tgcggccctg acctctcttc agaccgacac cgtgaccgtg
    19741 ccggataaaa agactgccac cgctgcgctg tcggctgaag atatcgcggc cgccaaactg
    19801 ctgggtaaaa ccgaagccga gttcctgaaa atgaaagagg aaatgcaata atgcctaccc
    19861 cgattacacc ggcgatgatc accgccctga tgacgggcta ccgttctgat tttcaggccg
    19921 ggatgtccat ggccccgtcg cagtataaaa aaattgcgat gaccgtgccc tccacatcga
    19981 aatccaacac ctacggctgg ctggggcagt tcccgcagtt ccgtgagtgg atcggctccc
    20041 gcgtcatcga gaagatgaaa gcctatggct atgccatcgt gaacaaaact tttgaaggca
    20101 ctgttgccat tagccgcgat gacttcgaag acgacaacct cggtatttat tccccgttgt
    20161 tccaggagat ggggcgcgct gcggcggtac aaccggatga gctggtcttt gctgccctgc
    20221 gtgacgggat cagtgcggcc tgctatgacg gtcagaactt ctttgatacc gaacatccgg
    20281 tttaccctaa agtggatggc tccggggatg cgcagatggt cagcaatatg tttgtggcaa
    20341 aaaccggctc tgtcggcgca caggctgatt acagcggtcc ggcctggtat ctgcttgact
    20401 gctcccgcgc ggtaaaaccg ctgatttatc aggatcgccg taaggctgaa ctggttgccc
    20461 agaccaaagt cgatgaaggt cgcgcattca ccgataatga atttgtgttt ggtgcatccg
    20521 cccgtcgcaa tgtgggctac ggcttctggc agatggccta catgatgcag tcaccgctga
    20581 cgctggatgc actgtggcac ggctggtcag ccatgcgcga atttaccgct gacggtggcc
    20641 gcaaactcgg catcaaaccc acccatattg ttgtccccac ctcgctggaa aaacaggcgg
    20701 tgcagctgct ggagcgtgaa ctgttcgcag acggaaacgc aaccgtctct aacgagatga
    20761 agggcaagct ggagctggtt gtcgcggatt atctgtaaac ggtgagccgg gcttacggcc
    20821 cggcccctgc ggagggcaaa tatgaatgaa catcacactg tggcagcaac agatgattca
    20881 ggtcttcagg tcagtggtga caattctgtc acggtgctgg cagaagtgcg ttgcagtcgt
    20941 tcggcgtttc gtcgtgcggg gttcctgttc acgcgtggac gtcagcaggt tgaggtcacc
    21001 ccggagcagc ttgcccgact ggaggcggag ccatgcctca ctgtgcgaat actacagacg
    21061 tctgctgatg atgcggggag cgtggcgggt gtggttcatg cagtggcctg tacagatt
//
LOCUS       DAZGXZ010000087        21101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000087 DAZGXZ010000000
VERSION     DAZGXZ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.087"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            98..622
                     /locus_tag="ACHKJF_003369"
     CDS             98..622
                     /locus_tag="ACHKJF_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFP0950676.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            706..1392
                     /gene="lpfB"
                     /locus_tag="ACHKJF_003370"
     CDS             706..1392
                     /gene="lpfB"
                     /locus_tag="ACHKJF_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP0950677.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1416..3947
                     /gene="lpfC"
                     /locus_tag="ACHKJF_003371"
     CDS             1416..3947
                     /gene="lpfC"
                     /locus_tag="ACHKJF_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFP0950678.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYSALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3958..5013
                     /gene="lpfD"
                     /locus_tag="ACHKJF_003372"
     CDS             3958..5013
                     /gene="lpfD"
                     /locus_tag="ACHKJF_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFP0950679.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            5019..5543
                     /gene="lpfE"
                     /locus_tag="ACHKJF_003373"
     CDS             5019..5543
                     /gene="lpfE"
                     /locus_tag="ACHKJF_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP0950680.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5796..7487
                     /gene="eptB"
                     /locus_tag="ACHKJF_003374"
     CDS             5796..7487
                     /gene="eptB"
                     /locus_tag="ACHKJF_003374"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP0950681.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7579..7655
                     /locus_tag="ACHKJF_003375"
     tRNA            7579..7655
                     /locus_tag="ACHKJF_003375"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7613..7615,aa:Pro,seq:cgg)
     gene            7690..7823
                     /locus_tag="ACHKJF_003376"
     ncRNA           7690..7823
                     /ncRNA_class="other"
                     /locus_tag="ACHKJF_003376"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8568..10175
                     /gene="dppA"
                     /locus_tag="ACHKJF_003377"
     CDS             8568..10175
                     /gene="dppA"
                     /locus_tag="ACHKJF_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP0950682.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVAA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10326..11345
                     /gene="dppB"
                     /locus_tag="ACHKJF_003378"
     CDS             10326..11345
                     /gene="dppB"
                     /locus_tag="ACHKJF_003378"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP0950683.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11355..12257
                     /gene="dppC"
                     /locus_tag="ACHKJF_003379"
     CDS             11355..12257
                     /gene="dppC"
                     /locus_tag="ACHKJF_003379"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP0950684.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12268..13251
                     /gene="dppD"
                     /locus_tag="ACHKJF_003380"
     CDS             12268..13251
                     /gene="dppD"
                     /locus_tag="ACHKJF_003380"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP0950685.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13248..14252
                     /gene="dppF"
                     /locus_tag="ACHKJF_003381"
     CDS             13248..14252
                     /gene="dppF"
                     /locus_tag="ACHKJF_003381"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP0950686.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14282..15553)
                     /locus_tag="ACHKJF_003382"
     CDS             complement(14282..15553)
                     /locus_tag="ACHKJF_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP0950687.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            16029..16136
                     /locus_tag="ACHKJF_003383"
     CDS             16029..16136
                     /locus_tag="ACHKJF_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0950688.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16513..16620
                     /locus_tag="ACHKJF_003384"
     CDS             16513..16620
                     /locus_tag="ACHKJF_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0950689.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16996..17103
                     /locus_tag="ACHKJF_003385"
     CDS             16996..17103
                     /locus_tag="ACHKJF_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0950690.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17190..18869)
                     /gene="bcsG"
                     /locus_tag="ACHKJF_003386"
     CDS             complement(17190..18869)
                     /gene="bcsG"
                     /locus_tag="ACHKJF_003386"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP0950691.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGDNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(18866..19057)
                     /gene="bcsF"
                     /locus_tag="ACHKJF_003387"
     CDS             complement(18866..19057)
                     /gene="bcsF"
                     /locus_tag="ACHKJF_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP0950692.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKAMATKK"
     gene            complement(19054..20625)
                     /gene="bcsE"
                     /locus_tag="ACHKJF_003388"
     CDS             complement(19054..20625)
                     /gene="bcsE"
                     /locus_tag="ACHKJF_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP0950693.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDEHLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            20898..21086
                     /gene="bcsR"
                     /locus_tag="ACHKJF_003389"
     CDS             20898..21086
                     /gene="bcsR"
                     /locus_tag="ACHKJF_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP0950694.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5361 a         5112 c         5528 g         5100 t
ORIGIN      
        1 attaaaaata attttctttt ttagcaccat ttgtataatc tgcgccccgt tgtatgaaca
       61 acatcaagca tatttatcta tataaatgga gttataaatg aaaaaggttc tgtttgcctt
      121 atctgcgctt gcgctgacct ctacttccgt attcgcagct gatgcgggcg acggtactgt
      181 taaattcacc ggtgagattg ttgacgccgc ttgtgttgtg tctccagaca ctcaaaaaca
      241 agaagttgtt ctgggtcagg tgaacaagtc tgtatttact gctatgggtg ataaatccac
      301 agcaacgccg ttcaaaatca aactggaaaa ctgcgatatc tccactttca aaaatgtgga
      361 aatcagcttt aacggcgttg gcgatgcaga caacagcaaa ctggtttctg taagcactga
      421 accaggtgcc gcaactggcg taggtatcgg tatttatgat aacaccaata ccctggttga
      481 cctgaatact ggtaaatctg ctactgtcct gaaagaaggt cagaccgttc tgtatttcac
      541 cgctaactat gtctctacca aagatgcagt aaccacgggt tacggtaacg ctgaagtcga
      601 cttcaacctg acttacaact aatcgatcct gacggaacga tcatctctca atggcaacag
      661 agactgtgtt gccattattc acaccaatga ctcagggacc gaatcatgaa acgtattagc
      721 tttgctgtgc tggcactttt aatgaccgca caacagtgtt ttgctggtgg cgtagcctta
      781 agcacgacgc gtgttattta tgatggcggc aagaaagaag cgtcgctgac cgttgaaaac
      841 cataataaaa atgaagagtt tttgatccag tcatggattg atgatgtgaa tggcaataaa
      901 aaaacaccgt ttattattac accgccatta ttcaaacttg atcctgataa aaataacgtc
      961 ttacgtatcg tcaatattga agatacctta cctaaagatc gtgaatccgt ttactggatt
     1021 aatgtaaagg ccattcctgc gaaaagtgct gaaaatgaaa ataaaaacgt cttacagata
     1081 gccgttcgca cccgtttgaa gttgttctat cgtccaaaag gtttgccggg aagcaacctc
     1141 gaggccatga aacagttacg tttccagcgt caaaataacc aaattgtggt taataacgac
     1201 tccgcttata acctgacctt taatcagttt tccattaatg gacaaaagat tgaacgggcg
     1261 ggaatggttc tcgcgaaagg aaagttaaac attaaccttc cagccggtac aggcaacgcg
     1321 cacgaagtga aatacagcat tattaatgat ttcggtgttg ctggcgaaat gctgacgaag
     1381 ccgattaact gatcctttta caaggcgctt ttactatgca ttggatgcat ttacctctct
     1441 atcactacag agcgcatttt tccttctctc tgctggcatt aacgattgcc agctcgttac
     1501 ctgcgtatgg cggaaaattt aaccctaaat ttctcgaaaa cgttcagggt atcgatcaac
     1561 acatcgactt gtcggtatac gactcacctg tcggccagca aatccccggt aaataccggg
     1621 tttcggtgtt tgtaaacgaa gaaaaaatgg cttcccggac gctggatttt agtaccgcca
     1681 gcgaagcgaa acgcaaagcc agcggtgagt cgcttatgcc ttgccttagc cgggtgcaac
     1741 tggaagaaat gggcgtgcgc gttgacagct ttccggcgct gaagatgtca ccaccagaag
     1801 cctgcgtagc gtttgatgaa atcattcccc aggccaccag ccgctttgat tttaatactc
     1861 agacgctgca tctttccttt ccacaggcgg caatgatgat gaccgctcgc ggcacggttg
     1921 atccttctcg ctgggacgaa ggaatacctg cgctgttgct ggattacagc tttagtggca
     1981 gcaatggacg caatgaaggt acaggttcat cttcagattc gaccagtgac agttattacc
     2041 tgaacctgcg cagcggtttg aacgttggtc cgtggcgttt acgtaataac agtatctgga
     2101 accgtaccga tggcaaaaac caatgggata acgttggtac atcgttaaac cgggcaatta
     2161 ttccacttaa atcacagata actctgggtg ataccgccac gcctggcgag atttttgata
     2221 gcgtacaaat gcgtggggcg ttactggcat ccgacgatga aatgttgccg gacagccagc
     2281 gtggctttgc tccggtagtg cgcggcatcg ccaaaagcaa cgctgaagtg agtatcgaac
     2341 agaacggcta tgtcatttat cgcacttttg ttcagccggg tgcatttgag attaacgatc
     2401 tctacgcaac ctccggcagc ggcgacctga cggtgatcat taaagaagcc gacggaagtg
     2461 aacaacgttt tatccagccg ttttcggcgg tggcgatttt ccagcgtgaa gggtatctga
     2521 aatacagtct ggctgccggt gaatatcgtg ctggtaatta cgacagcgat aaaccacatt
     2581 tcgggcagtt taccgccatg tacggcttgc cgtggggtat gaccgcctac ggtggtgcat
     2641 tgttgtcggc agattacagc gctttagctc tgggcctggg gaaaaacttc ggtaccattg
     2701 gcgcagtatc ggtggatgtc actcaggcga aaagtcagtt acgcaacaat gaaaaagagg
     2761 aaggccagtc ttatcgcttc ctctattcga aaagttttga gggtggcact gacctgcgtc
     2821 tgttgggcta caaatactca accagcggct actacacctt ccaggaagca actgatgttc
     2881 gcagtgacgc cgacagtgat taccgccgtt atcacaagcg cagccagata cagggcaata
     2941 ttacccagca actgggagac tacggctcgg tttatttcaa tatgacgcag caggattact
     3001 ggaacgttga cggtaaggaa aactccttaa gcgccggtta tcacggtcac attggtcggg
     3061 tgaattacag cgtggcctat acctggacga gaagtccgga atgggaagaa gacgatcgct
     3121 tgtggtcatt cagtgtttcc atccctctgg ggggcgcgtg gagcagttat cgcatgacta
     3181 ccgaccagaa tggcaagacg tcgcaacagg cctctgttag cggcacgtta ctggaagatc
     3241 gtaacctgag ctataacgtt cagcagggtt acaccagtaa tggcgtaggc tacagtggca
     3301 gcgtcaacat ggggtatatg ggtggctctg gtaacattga cgtaggctat aactattcta
     3361 aagataacca acaggttaac tatggcgtgc gcggcggtgt catcgttcac agtgaaggta
     3421 taacgctttc gcagccttta ggcgaaagtc tggcgattgt ttctgcaccg ggcgcgcgtg
     3481 gtggacatgt ggttaacagc agcggcgtag aagttgactg gatgggcaac gccgtggtgc
     3541 cttatctcac accatatcgt gaaaccattg tggaattgcg tagcgacacg ttggggcaaa
     3601 acgttgaact acaggaagcg ttccagaaag tcgtgcctac tcgtggtgca gttgtgcgtt
     3661 cccgctttga tacccgcgtc ggttaccgtg tgctgatgag cttaaaacag gcgaatggca
     3721 acgcagtacc gtttggtgca accgcagcgt taattgatga gagcaaacct gccagcagca
     3781 ttgtgggtga agaaggacag ctttatatca gcgggatgcc agaagagggc gaattgcagg
     3841 tgagctgggg gaacgagcag gcgcaacgtt gccgtgtgcc attccgcttg ccagagaaca
     3901 aggacaatgc ggctatcgtg atggttaatg cggtatgcga gaaataagga tatttagatg
     3961 aaaattaaaa atttgttggc aggtatggtg ctgttaggga cgagcacttt tgctggaaat
     4021 atatgggctg cggattgggg accatgtcag acatcaaatg gggtggccca tgagtatagt
     4081 tttgatttcg tacagactat ccaggtacct tcagaaaata aggctgggaa aatattaact
     4141 cagccctttg ctttaggtac aaaatattcc gcttattgtg aatgcccgga tcccattcca
     4201 gataatggag ttgtgactta ttttaaaggt gtaacgcttc tgcctgagcc tggaactgta
     4261 gatgggtact ataaattcaa taattacatt gatattctca ccaaaatata tgtctacaca
     4321 caattagata tcccggtgcc ttttaccgat cgaagcaatg gcacggcaca gaaagaatgt
     4381 acgccctata ctgctaacaa ttgggggaca ggtggtaaag gaagtatcag tatttatatt
     4441 tcacatcctt ttgttgggca aatgattatt cctaaaacac gagttgcgtc cctctttggg
     4501 acaaaaaaga aaggtgttta caatgatagt cagcccatgg ccaatgtcag tatttccggg
     4561 agtattacag ttactcaagg ctgtgaactt gccgctggca ccgtactgga tattccattc
     4621 ggagaatatc aggcccatga ttttaaaggg cgtgccggac aaccaccaca aaatgttcag
     4681 aaggtacaaa aagaattaag ctttgactgc aataatattt cagatggcgt caaaatttat
     4741 ctcagtattg atgcaacacc aaatactgcc tatcccagcg caattgattt aggcaacgcc
     4801 gatgttggtg cagtcattga ggacggtaaa ggtaatattc ttaagccgaa tgacagtaat
     4861 agcttattag aaatgaaccc aggctcgctg tatgaagatg tcaaacgcaa agcgacaacc
     4921 acaattaccg cttatcccgt tagcactacc ggaaaattac ccgctgccgg ggattatagc
     4981 ggtattgcta ccatacatgt cgaactggag taacagtcat gaagcgaaca atacttgcgt
     5041 tatgcctgct gcctctttcc ttttcgtcat tcgcggcaac cacggattta gggccgaaag
     5101 gaacattgaa attcaccctt aaaatcagcc agggtgcttg cgaactggtg aaagacagcg
     5161 ttgaggttga catgggcacc gcggttctca aaaagccggt aagaatcgga acggaaatta
     5221 atcccactcc tttttccatc ggcctgaaaa actgtagcga agtggtcaga gcctacgtga
     5281 ctatggacgg tacacccaat gcagacgact caaatctttt tgcgctggat gcgggtggcg
     5341 ctacaggcgt tgggctgaaa atcaaaaccg ctgctggagt acaacagttc ccggaaaata
     5401 caacgccgac accggttgag tttgcaatta acggtgatgg cgcacatcaa ctgaactata
     5461 tcgccagcta cgtgcctatt cgcgccgatg caactattgg tcgcgcagat gcaacggtcg
     5521 actttactgt acagtacgaa taatttcaca tcttaagggc cagttaactg gcccttttta
     5581 ctgctttttg taacgctaaa gtctcttttc aaacttgcat ttttgtaaat ttgtgcttca
     5641 tgcacactct ttccccacac tttttcgctt tgctgtggtc tacttattcg cgcgtgtaga
     5701 ttttacttat ctgactacct ccgcactttt cccctgccgg gcctgaaaag ccgctaagca
     5761 gggtgttatc acctgtttgt ccagggtttg tttgcatgag atacatcaaa tcgattacac
     5821 agcagaagct gagctttttg cttgcaatct atattggcct ttttatgaat ggcgcggttt
     5881 tttatcgccg cttcggcagc tatgcgcacg attttaccgt ctggaaaggc gtttctgctg
     5941 ttgttgaact ggccgccacc gtactggtga ccttcttttt actacgtctt ctatcgctgt
     6001 ttggccgccg cagctggcgt attctggcgt cgctggtggt gctcttttcc gcaggtgcca
     6061 gctattacat gaccttcctt aatgtggtca ttggttatgg catcatcgct tccgtcatga
     6121 ccaccgatat cgacctgtca aaagaagttg ttggtctgaa ctttattctc tggttaatcg
     6181 ccgttagtgc attgcctctt atccttatct ggaataaccg ctgtcgctac accttgctcc
     6241 gacaactgcg aaccccaggg cagcgtattc gcagcctggc ggtcgtcgta ctggcgggta
     6301 ttatggtttg ggcaccgatt cgtttgctgg atatccagca gaagaaagtg gagagggcga
     6361 ccggcgttga tttgccgagt tatggcggtg tcgtagcgaa ctcttatctg ccatcaaact
     6421 ggctttctgc gttggggctg tatgcctggg cgcgggtcga tgaatcttcc gataataatt
     6481 cattgcttaa tccggcgaag aaattcactt atcaggcacc gcaaaacgtt gatgacactt
     6541 atgtcgtgtt tatcatcggt gaaaccacgc gttgggacca tatgggtatt ttcggctatg
     6601 agcgtaatac cacgccgaaa ctggcccagg agaaaaatct ggcggcgttc cgtggttact
     6661 cctgtgatac cgcaaccaaa ctctcactgc gttgcatgtt tgtacgtcag gggggcgcgg
     6721 aagataatcc gcagcgcaca ttaaaagaac agaacatttt cgcggttctg aagcagttag
     6781 gattcagttc tgacctctac gctatgcaga gcgaaatgtg gttctacagc aacacgatgg
     6841 cggacaacat tgcttatcgt gagcagattg gtgcggagcc acgtaatcgt ggcaagccgg
     6901 tagatgatat gttgctggta gacgaaatgc agcaatcgct agggcgcaac ccggatggta
     6961 agcatctgat cattctgcat accaaaggtt cgcattttaa ctacacccag cgttatccgc
     7021 gtagcttcgc gcagtggaag ccggaatgta ttggtgttga tagcggctgt accaaagcgc
     7081 agatgatcaa ctcctatgac aactcggtga cctatgtgga tcactttatc tccagcgtga
     7141 ttgatcaggt tcgcgataag aaagcgattg tgttctacgc agctgaccac ggtgagtcaa
     7201 ttaatgaacg cgagcacctg cacggcacgc cgcgtgaact ggcaccgccg gagcagttcc
     7261 gcgtaccgat gatggtctgg atgtcagata aatatctgga aaatccggcc aatgcgcagg
     7321 cgtttgcgca gctgaaaaaa gaagccgaca tgaaagtgcc acgccgtcac gtagagctgt
     7381 acgacaccat catgggttgt cttggctata cttcaccgga tggtggaatt aacgaaaaca
     7441 acaactggtg tcacatcccg caggcaaaag aggcagcggc taactaagcg gcctgctgac
     7501 tttctcgccg atcaaaaggc attttgctat taagggattg acgagggcgt atctgcgcag
     7561 taagatgcgc cccgcattcg gtgattggcg cagcctggta gcgcacttcg ttcgggacga
     7621 aggggtcgga ggttcgaatc ctctatcacc gaccaaattc gaaaagcctg ctcaacgagc
     7681 aggctttttt gcatctgcag ctcatgagga tgagaacctc cgggggcagg gaggttcgac
     7741 tcgagcgaag cgagagaacg ttgcgccagc aacggcccgc agggcgagcc acgaagtggc
     7801 gagtaatcct ctatcaccga ccaaattcga aaagcctgct cagcgagcag gcttttttgc
     7861 atctgcagct catgaggatg agaacctccg ggggcgggga ggttcgactc gacgtaggcc
     7921 tgataagacg cgccagcgtc gcatcaggca acggcaccgc acttattgtc ggatgcggcg
     7981 cgaccgcctt attcgaccaa cacaccacac attttcctgc caactttgcg ccccatatct
     8041 cactatccat taacggattt gtgacagaaa ccattgtctt tttttatatt tgcttaaatc
     8101 tttttttgcg ttacttatgg ctggcgctgg cattttccgc tgtgcgtttt agcgttcatg
     8161 gtattaatcg ctcagatatc caccttttcc tcagattttt ttcattaaat gcataaaact
     8221 taatcaccaa atgttgctaa atattaagct gattgttctg gtcatgttgg agtagcacaa
     8281 atttctctgc tgcaaatagc tgtttgaagt ttttttaatc tttgtttgtg gattctcacc
     8341 gttggtgtaa atcccggttg gctgtattga cgttttcaca ttctgttgac agattgtagg
     8401 tcacgagggg cattttatgg aggatccgca ctgttacact gatgttaatt agtacggcat
     8461 ccccacctca taacgttgac ccgaccgggc aaaaaacaaa aaaggtcagg cagcgacaac
     8521 ccactgcaaa gggttaaaac aacaaacatc acaattggag cagaataatg cgtatttcct
     8581 tgaaaaagtc agggatgctg aagcttggtc tcagcctggt ggctatgacc gtcgcagcaa
     8641 gtgttcaggc taaaactctg gtttattgct cagaaggatc tccggaaggg tttaacccgc
     8701 agctgtttac ctccggcacc acctatgacg cctcttccgt accgctttat aaccgcctgg
     8761 ttgaatttaa aatcggcacc actgaagtga tcccgggcct cgctgaaaag tgggaagtca
     8821 gcgaagacgg taaaacctat accttccatc tgcgtaaagg tgtgaagtgg cacgacaata
     8881 aagaattcaa accgacgcgt gaactgaacg ccgatgacgt ggtgttctcg tttgatcgtc
     8941 agaaaaacgc gcaaaacccg taccataaag tttctggcgg cagctacgaa tacttcgaag
     9001 gcatgggctt gccggagctg atcagcgaag tgaaaaaggt ggacgacaac accgttcagt
     9061 ttgtgctgac tcgcccggaa gcgccgttcc tcgctgatct ggcaatggac ttcgcctcta
     9121 ttctgtcaaa agaatatgct gatgcgatga tgaaagccgg tacaccggaa aaactggacc
     9181 tcaacccaat cggcaccggt ccgttccagt tacagcagta ccaaaaagat tcccgtatcc
     9241 gctacaaagc gtttgatggc tactggggca ccaaaccgca gatcgatacg ctggttttct
     9301 ctattacccc tgatgcttcc gtgcgttacg cgaaattgca gaagaatgaa tgccaggtga
     9361 tgccgtaccc gaacccggca gatatcgctc gcatgaagca ggataaatcc atcaacctga
     9421 tggaaatgcc ggggctgaac gtcggttatc tctcgtataa cgtgcagaaa aaaccactcg
     9481 atgatgtgaa agttcgtcag gctctgacct acgcggtgaa caaagacgcg atcatcaaag
     9541 cggtttatca gggcgcgggc gtggcagcga aaaacctgat cccgccaacc atgtggggct
     9601 ataacgacga cgttcaggac tacacctacg atcctgaaaa agcgaaagcc ttgctgaaag
     9661 aagcgggtct ggaaaaaggt ttctccatcg acctgtgggc gatgccggta caacgtccgt
     9721 ataacccgaa cgctcgccgc atggcggaga tgattcaggc agactgggcg aaagtcggcg
     9781 tgcaggccaa aattgtcacc tacgaatggg gtgagtacct caagcgtgcg aaagatggcg
     9841 agcaccagac ggtaatgatg ggctggactg gcgataacgg ggatccggat aacttcttcg
     9901 ccactctgtt cagctgcgcc gcctctgaac aaggctccaa ctactcaaaa tggtgctaca
     9961 aaccgtttga agatctgatt caaccggcgc gtgctaccga cgaccataac aaacgcgttg
    10021 aactgtacaa gcaagcgcag gtggtgatgc acgatcaggc tccggcactg atcatcgctc
    10081 actccaccgt gtttgaaccg gtacgcaaag aagttaaagg ctatgtggtt gatccattag
    10141 gcaaacatca cttcgaaaac gtctctatcg aataattaaa agccataccc ggtggtgctg
    10201 tgcctctgcg tgcagcgcca tccggcagca atacttcatt ccctgcccga cctgggcggg
    10261 aattgatttg tgagcaatac agacacgcag ttccaggctg cgggtcacta tagagaatcc
    10321 gggttatgtt gcagtttatt ctccgacgtt tgggactcgt catccccacg tttatcggta
    10381 ttacccttct cacatttgcc tttgtccaca tgatcccggg cgatccggtg atgatcatgg
    10441 caggcgaacg tgggatctcc cctgagcgtc acgcgcagct gctggctgaa ctcggcttag
    10501 ataaaccgat gtggcagcag tatctccatt acatttgggg cgtaatgcac ggcgatctag
    10561 gcatttcaat gaaaagccga attccagtat gggaagagtt cgtgccgcgc tttcaggcca
    10621 cgctggaact tggcgtctgc gcgatgattt ttgctacggc agtcggtatt ccggttggtg
    10681 tgctggctgc ggttaaacgc ggttccattt tcgatcacac agcggttggc ctggcgctga
    10741 ccggctactc gatgccgatc ttctggtggg gcatgatgct gatcatgctg gtttcggtgc
    10801 actggaacct gacgcccgtc tccggtcgcg tgagcgatat ggtgttcctc gatgactcca
    10861 atccgttaac cggttttatg ctaatcgaca ccgccatctg gggtgaagac ggcaacttta
    10921 tcgatgccgt cgcccatatg atcttgcctg ccattgtgct gggtactatt ccgctggcgg
    10981 tcattgtgcg tatgacacgc tcctcgatgc tggaagtgct gggcgaggat tacatccgca
    11041 ccgcgcgcgc caaagggcta acccgcatgc gggtgattat cgtccatgcg ctgcgtaacg
    11101 cgatgctgcc ggtggtgacc gttatcggcc tgcaggtggg aacattgctg gcgggggcga
    11161 ttctgaccga aaccatcttc tcgtggcccg gtctgggacg ctggttgatt gacgcactgc
    11221 aacgccgcga ctatccggta gtgcagggcg gcgtattgct ggtggcgacg atgattatcc
    11281 tcgtcaactt gctggtcgat ctgctgtacg gcgtggtgaa cccgcgtatt cgtcataaga
    11341 agtaagggga catcatgtca caggttactg aaaataaagt gattagcgca ccggtgccga
    11401 tgaccccgtt acaggagttc tggcactatt ttaaacgcaa caaaggcgcg gtcgtcgggc
    11461 tggtttacgt cgtcatcgtg ctgttcatcg cgatctttgc caactggatt gcaccctata
    11521 acccggcgga acagttccgc gatgcactgc tcgccccgcc agcctggcag gaaggcggca
    11581 gcatggcgca cttgctgggc accgatgacg taggccgtga tgtgctgtcg cgcctgatgt
    11641 acggtgcgcg cctgtcgctg ctggttggct gtctggtagt tgtgttatcg ctgattatgg
    11701 gcgttattct cggcctgatc gccggttact ttggcggcct ggtcgataac atcattatgc
    11761 gcgtggtcga tatcatgctg gcgctgccaa gtctgctgct ggcgctggtg ctggtggcaa
    11821 ttttcggccc gtcgattggt aacgccgcgc tggcactgac cttcgttgcc ttgccgcact
    11881 atgtgcgctt aacccgcgcc gccgtgctgg tggaagttaa ccgcgattac gtcaccgcgt
    11941 ctcgcgtggc gggtgccggg gcgatgcgtc agatgtttat taacatcttc ccgaactgcc
    12001 ttgcgccgct gattgttcag gcgtcgctcg gtttctctaa cgccattctc gatatggctg
    12061 ctcttggttt cctcggcatg ggggcacagc cgccaacgcc tgagtggggc accatgctct
    12121 ccgacgtgtt gcagttcgcg caaagcgcct ggtgggtcgt gaccttcccg ggtctggcga
    12181 tcctgctgac ggtgctggca tttaacctga tgggtgacgg tctgcgtgac gcgctcgatc
    12241 ccaaactgaa gcagtaagag gttcgagatg gcgttattaa atgtagataa attatcggtg
    12301 catttcggcg acgaaagcgc gccgttccgc gccgtagacc gcatcagcta cagcgtaaaa
    12361 cagggcgaag tggtcgggat tgtgggtgag tccggctccg gtaagtcggt cagttcactg
    12421 gcgattatgg ggctgattga ttatccgggc cgcgtaatgg cagaaaaact ggagtttaac
    12481 ggccaggatt tgcagcgtat ctcagaaaaa gagcgccgca acctggtggg tgccgaagtg
    12541 gcgatgatct tccaggaccc gatgaccagc cttaacccgt gctacaccgt gggtttccag
    12601 attatggaag cgattaaggt gcatcagggc ggcaacaaaa gtacccgccg tcagcgagcg
    12661 attgacctgc tgaatcaggt cggtattccc gatccggcat cgcgtctgga tgtttacccg
    12721 catcagcttt ccggcggcat gagccagcgc gtgatgatcg ccatggcgat tgcctgtcgg
    12781 ccaaaactgc tgattgccga tgaaccgacc accgcgctgg acgtgaccat tcaggcgcaa
    12841 atcatcgaac tactgctgga gctacagcag aaagagaaca tggcgctggt gttaattacc
    12901 catgacctgg cgctggtggc ggaagcggca cataaaatca tcgtgatgta tgcaggccag
    12961 gtggtggaaa ccggtgatgc gcacgccatc ttccatgcgc cgcgtcaccc gtatactcag
    13021 gcattgctgc gtgcgctgcc agaatttgct caggacaaag aacgtctggc gtcgttgcca
    13081 ggtgtcgttc ccggcaagta cgaccgcccg aacggctgcc tgcttaaccc gcgctgcccc
    13141 tatgccactg acagatgtcg cgctgaagaa ccggcgctga atatgctcgc tgacgggcgt
    13201 cagtccaaat gccattaccc acttgatgat gccgggaggc cgacactatg agtacgcaag
    13261 aggccaccct gcaacaaccg ctgttgcagg ctatcgacct gaaaaaacat tatccggtga
    13321 agaaaggcat gttcgcgccg gaacgtctgg ttaaagcgct ggatggcgtt tcgtttaacc
    13381 ttgaacgtgg caaaacgctg gcagtagtgg gcgaatctgg ctgcggtaaa tcgaccctcg
    13441 gtcggttgct gacgatgatt gaaatgccca ccggtggcga gctgtattac caggggcagg
    13501 atctgcttaa gcacgatccg caggcgcaga agctgcgtcg gcagaaaatc cagatcgtct
    13561 tccagaaccc ttacggttcg ctgaatccgc gtaaaaaagt cgggcaaatt cttgaagagc
    13621 cgctgctgat caacaccagc ttaagcaaag aacagcgtcg ggaaaaagcc ctgtcgatga
    13681 tggcgaaagt cggcctgaaa accgagcact atgaccgcta tccgcatatg ttctccggcg
    13741 gtcagcgtca gcgtatcgcc atcgcccgtg gtctgatgct cgacccggat gtggtgattg
    13801 ccgatgaacc ggtttctgcg ctggatgttt cagtgcgcgc gcaggtgctg aatctgatga
    13861 tggatttgca gcaggagttg gggctgtctt atgtctttat ctcccacgac ctgtcggtgg
    13921 tggagcacat tgctgatgaa gtgatggtga tgtacctggg ccgctgcgtg gagaagggaa
    13981 cgaaagatca aatcttcaat aacccgcgcc atccgtacac tcaggcgcta ctttccgcaa
    14041 cgccgcgcct gaacccggac gatcgccgcg agcgcatcaa gctcagcggt gaactaccaa
    14101 gcccactgaa tccaccgccg ggttgcgcct tcaacgcccg ctgtcgtcgg cgcttcggcc
    14161 cctgcaccca gttgcagccg cagctaaaag actacggcgg tcaactggtc gcttgttttg
    14221 ctgttgatca ggatgaaaat ccgcagcgtt aactcacaaa accggggcca tgcgctccgg
    14281 tttactcaat cagcttcaac agtggcgaca agcatagcaa aatgccgtaa agcagaatta
    14341 gccaggcttt aaatccgcga agtttttcca gttgtgcgac tttatagatc aggaaaaacg
    14401 gaatgaggca cgacacaatg ccatataacg ggcttcccaa ctgaaagaac accagcaccg
    14461 atacacgaaa cgaaacccaa atcgtcaatg taatgacgat aaaagcgcag atcgccagag
    14521 tcagcatgcg tgagttaatt ttcttggtat caataattcg gcttaacaga ttgagaataa
    14581 tgcctttaat agcctcgtgg aaaccgagat aaatgccaaa gaatgcggtc agtacggcaa
    14641 agatattcag caccgtagag gtgatatgaa tgatatgtcc agggatcacc tgcgcggcca
    14701 gcgccagtgc cgagatattt tgttcaaagg cagaaacggc ttcttcgtgg ctaattgaga
    14761 aggtaaacga aaaggcaaaa aacaggatca ccgcgatgag cgtgatataa ctaattcggt
    14821 gggtacgcag cgcgagccgg gttgccagta ctttatctgc ttcccgttta cgataggcaa
    14881 tattcattgg gttaagtacc tgaataaata ccgcagaaaa gaagcaaaat ggaatggtaa
    14941 gcaggacatc gcggaaaaag acggaggctt gcgggaaggc ggttatattg gcgaaattcc
    15001 agtgcgggat catcgcaaaa ccgaacacga caataatccc tactttgacc accaccattg
    15061 gcccggaaat cttaaacagt aatcgttcac caccagatgc aatcgccacc agtacggcga
    15121 aaatagcgac tttataaagt agagattgtg aaagatcggc atcggttaaa ccgaaggttt
    15181 tcaggtacga ggcgctgtcg aaaaccacgg agagagagta gataaaaatc ccgtggataa
    15241 tcatcaaaaa gtagataacc ccgaggaaaa ttccccagtt cttccccagg taatgactga
    15301 taatatcggt gtagtcatta caggaatcgc tttcagaaag ggtttttaaa taaatgtcct
    15361 gcactaccca ggtggcagga taagcaatga tcgctgcggt aataaatacc caaattccct
    15421 tcaagccaat ttgtactggc atcagcacgg ttccggcacc aatcgccatg cctatgcata
    15481 ataaaaccca gccgaagtcg tagcgtgtaa acggcaattt ctgattgtgt ttcgatagtg
    15541 tgttgtgctg cataaagtcc tgtccagaaa gaagtcggcc tcgcaaaaat gaataaaccc
    15601 agtttctctt gcgcaacagg tggagaagtc gaatacgcag tatgtaaaaa atgaggcaac
    15661 agattcactg gggaggtcgc gaggaggcgc aattctacaa aattgcgcat ttcactacca
    15721 gtctggagtc agcccggata tgaactgcaa ctattgcaaa taaagaaatt aagctggaag
    15781 gcatcgcgca atgaggaaga ttgccgggtg ctgacagtaa aaagcaatcg taaaatcagc
    15841 ggcaagccgg aagggttccg gtgaggcgca atgttgcggg ggctttatcc ctggtggcat
    15901 tggttgctgg agagagaaaa cccccgcacg ttgcaggtat atacctgaca acataacggg
    15961 ggctaatctt gaacctagac cactcaagaa tagccgcgaa acgttgtcat tacaacacag
    16021 gcggctatat gacgctcgca gagctgggca tggccttctg gcatgattta gcggctccgg
    16081 tcattgctgg cattcttgcc agtatgatcg tgaactggct gaacaagcgg aagtagcgtg
    16141 tcatgtgggc gtcaggctgc cgtaatggca atttgcgccc ggaccaggcc gcaggagtga
    16201 aactctgcgg cctttttcgt aagaaaaatg caacggttgc aagcggataa tattctgcgg
    16261 aatgcgcagc acagagtgaa gatgagacgg attccggaac agaaaaacag tcgtagaatc
    16321 agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa tccctggtgg
    16381 cattggttgc tggagagaga aaacccccgc acgttgcagg tatgcacctg acaacaccac
    16441 ggggggccat tcttgcatcc aaacaataca agaatagccg cgaaacgttg tcattacaat
    16501 acaggcggct atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct
    16561 ccggtcattg ctggcattct tgccagtatg atcgtgaact ggctgaacaa gcggaagtaa
    16621 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
    16681 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacgg ttgcaagcgg ataatattct
    16741 gcggaatgcg cagcacagag tgagtaagtg atggatttcg gaacagaaaa acagtcgtag
    16801 aatcagcggc aagccggaaa ggttccggta aggcgcaatg ttgcgggggc tttatccctg
    16861 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
    16921 taacgggggc taatcttgaa cccaagcaac tcaagaatag ccgcgaaacg ttgtcattac
    16981 aacacaggcg gctatatgac gctcgcagag ctgggcatgg ccttctggca tgatttagcg
    17041 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
    17101 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
    17161 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
    17221 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
    17281 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
    17341 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagccaggaa gctgctcggt
    17401 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
    17461 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
    17521 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
    17581 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
    17641 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
    17701 tagaacgtgg cactacggct atttttatct ttttcggtaa cgtccagcca gcggttaagc
    17761 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
    17821 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
    17881 ccgaactggc cgttatgccc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
    17941 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
    18001 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
    18061 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
    18121 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
    18181 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
    18241 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
    18301 accggtgcgc cacccgtcgc cgcaacggtt gccgctgcgt tatcacccgt cgttgttaca
    18361 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
    18421 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
    18481 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
    18541 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
    18601 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
    18661 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgcggca
    18721 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
    18781 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
    18841 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc catggctttt tccgtgcggc
    18901 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaacaaacgt aaggtgtcgc
    18961 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
    19021 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
    19081 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
    19141 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
    19201 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
    19261 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
    19321 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
    19381 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
    19441 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
    19501 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
    19561 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
    19621 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
    19681 ggccaataac aaatgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
    19741 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
    19801 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
    19861 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
    19921 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
    19981 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
    20041 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
    20101 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
    20161 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
    20221 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
    20281 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggt
    20341 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
    20401 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
    20461 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
    20521 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
    20581 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
    20641 gattatcagt ttactagcga aagctaagaa ataaacctac cattgaaatt aaaacttatc
    20701 agatttagca tgtgaatcaa tttagaaggt cacaagacgt gtgattttag tcttttcgtt
    20761 tttaactttt tctgttgcgt aacggaaagt caaaaagtga gcaaattccc gtctcgccgt
    20821 aaacaataaa ccggcgaaat gctcacggtt agaattgtct catgaacggt acggttattt
    20881 catagggatc aagcaaaatg aataacaatg aaccagatac tctgcctgat cccgcgatag
    20941 gctatatctt ccagaatgat attgtggcgt taaagcaggc attttcactg cctgatattg
    21001 attatgccga tatttcccaa cgcgaacagt tggccgcggc attaaaacgc tggccgttgc
    21061 tggcagagtt tgcgcaacaa aagtagggga ttggtgaatg g
//
LOCUS       DAZGXZ010000088        21069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000088 DAZGXZ010000000
VERSION     DAZGXZ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.088"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            323..823
                     /gene="tssB"
                     /locus_tag="ACHKJF_003390"
     CDS             323..823
                     /gene="tssB"
                     /locus_tag="ACHKJF_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0950695.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSSLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            858..1082
                     /locus_tag="ACHKJF_003391"
     CDS             858..1082
                     /locus_tag="ACHKJF_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950696.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1133..2608
                     /gene="tssC"
                     /locus_tag="ACHKJF_003392"
     CDS             1133..2608
                     /gene="tssC"
                     /locus_tag="ACHKJF_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0950697.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2615..3028
                     /gene="tssE"
                     /locus_tag="ACHKJF_003393"
     CDS             2615..3028
                     /gene="tssE"
                     /locus_tag="ACHKJF_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0950698.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3032..4882
                     /gene="tssF"
                     /locus_tag="ACHKJF_003394"
     CDS             3032..4882
                     /gene="tssF"
                     /locus_tag="ACHKJF_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0950699.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFTRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4846..5928
                     /gene="tssG"
                     /locus_tag="ACHKJF_003395"
     CDS             4846..5928
                     /gene="tssG"
                     /locus_tag="ACHKJF_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0950700.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5953..7233
                     /gene="tagH"
                     /locus_tag="ACHKJF_003396"
     CDS             5953..7233
                     /gene="tagH"
                     /locus_tag="ACHKJF_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP0950701.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7230..7754
                     /gene="tssJ"
                     /locus_tag="ACHKJF_003397"
     CDS             7230..7754
                     /gene="tssJ"
                     /locus_tag="ACHKJF_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0950702.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7757..9088
                     /gene="tssK"
                     /locus_tag="ACHKJF_003398"
     CDS             7757..9088
                     /gene="tssK"
                     /locus_tag="ACHKJF_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0950703.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9093..9854
                     /gene="icmH"
                     /locus_tag="ACHKJF_003399"
     CDS             9093..9854
                     /gene="icmH"
                     /locus_tag="ACHKJF_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP0950704.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRDYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWLCFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTIKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9863..12646
                     /gene="tssH"
                     /locus_tag="ACHKJF_003400"
                     /gene_synonym="clpV"
     CDS             9863..12646
                     /gene="tssH"
                     /locus_tag="ACHKJF_003400"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0950705.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWQKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGHVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAEPADEAGSEQPEETAETVSPVQRLAH
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIM
                     SRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12643..13386
                     /gene="vasI"
                     /locus_tag="ACHKJF_003401"
     CDS             12643..13386
                     /gene="vasI"
                     /locus_tag="ACHKJF_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP0950706.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13391..14803
                     /gene="tssA"
                     /locus_tag="ACHKJF_003402"
     CDS             13391..14803
                     /gene="tssA"
                     /locus_tag="ACHKJF_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0950707.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14912..18346
                     /gene="tssM"
                     /locus_tag="ACHKJF_003403"
     CDS             14912..18346
                     /gene="tssM"
                     /locus_tag="ACHKJF_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP0950708.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISEY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVPRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18357..19806
                     /locus_tag="ACHKJF_003404"
                     /pseudo
     CDS             18357..19806
                     /locus_tag="ACHKJF_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19733..20215
                     /locus_tag="ACHKJF_003405"
     CDS             19733..20215
                     /locus_tag="ACHKJF_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0950709.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20259..>21069
                     /locus_tag="ACHKJF_003406"
     CDS             20259..>21069
                     /locus_tag="ACHKJF_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950710.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLK"
BASE COUNT         5043 a         5686 c         5756 g         4584 t
ORIGIN      
        1 agcaatcatt agattagctt atgtttaata aaattattat ataattcaat aagatgcata
       61 aaaactctat atttaaatta ttgccataat acacctgacg cgaattgcac aggttcatac
      121 aatctattta acgcttgcag aacacccctc tttacgatcg ataaaaagtc cttttcttat
      181 ttattttgaa gcgtaaaatg cggggtgatg aaggagtggt gattttattt tcaccgccat
      241 caaggcggca tttccgatat tttttgcggt acgaatatgc cgtgttcatg gcctgtacga
      301 gataacatga cggatcactt ttatgagcaa aaaatttgaa ggcagcgtcg cgcctcgcga
      361 gcgtattaat atatcgtatg tcccaaaaac agatggccag acggctgaag tggaacttcc
      421 gcttaacatg ctcgttgtcg gcgataccgg aaatacgcag gaaacatcct cactggatga
      481 acgtcaggca gtttccgtta acaagcacaa tttcggtgca gtgatggctg aagcagccat
      541 tggcctcaat tttactgtcc ccgccaccct gaagggaagc acaacagacg atgaactgaa
      601 tgtggcgctg aatatcaaat cactggatga tttttcacca gatagcgtgg cccggcaggt
      661 tccggaagtg aataaattgc tggaactgcg tgaagcactg actgcactga aggggccgat
      721 gggtaacctt ccggcattcc gtacccagct ccaggcattg ctggaaaatg aagagtcccg
      781 cgagcagttg ctcaaagaaa tcggtctggt gagcaataaa taaccaccac tatttcatta
      841 aacagggtta tccaggcatg acggcacaca aaaatatctc cgggacatac cacctctctg
      901 ttgcagacat tctgcaggtg gtttaccagg tatgtttcag cccgtccgtt gaaatcaacc
      961 aggatggtgt ggctgcgctg attacaacac tggatcgccg tatcagtgat ctattggatg
     1021 agattatcca tttttgtgag tttcagcaga gcgcgtcgca ctggcagcgc gtacttcact
     1081 gacggcgaaa agccggtttc agaaacacag ctaaaacaga aggattttca gtatgtcttt
     1141 acaggaagag gaactcgtat ccagtcatgc cgggcagcct gagcaggcat cgtcgctgct
     1201 tgatcaaatc atggcacaga cccgtattca gcccggttcg gaaggttatg acgttgcccg
     1261 ccagggtgtg acagccttta ttgccagcat cctgcaatcc actgcatctg ccgaaccggt
     1321 caataaactg gccgtggaca gcatgattgc agatattgat gaacgcatca gccgccagat
     1381 ggatgtcatc atccatgcgc ctgcctttca gcaggtcgag tcgttctggc gctcactgaa
     1441 aaccatggtg gatcgcgttg atttccgtga aaacatcaag gtcaacgtgc ttcatgtcac
     1501 caagcaggaa cttctggaag actttgagtt tgcgccagaa attattcagt ccggatttta
     1561 taagcacgtt tattcgtccg gtttcggaca gtttggcggt gaacccatcg ctgccgtgct
     1621 gggcgcttat gagttcaaaa ataccgcgcc tgacatgaaa ctgctgcaat acgtcagtgc
     1681 cgtgggcgca atggcgcacg caccgttcct gtcctccgtt tccccggagt ttatggggct
     1741 gaactcgtgg accgaactgc ctaatatcaa agatctttat gccatctttg aaggtccggc
     1801 ctacaccaaa tggcgcgccc tgcgtgactc ggaagattcg cgctatttag ggcttacggc
     1861 accccgcttc ctgcttcgcc agccctattc accaacagat aaccctgtta agaactttaa
     1921 ctattacgaa gatgtcagcc agaaccacga agattatctg tggggtaata cggcctggat
     1981 gctggcatgc aatatcgcag acagttttgc caaataccgc tggtgtccaa acattatcgg
     2041 tccgcaaagc ggcggcgcag tgaaagatct gccggtgcat ctgttcgaaa cgatggggca
     2101 gattcaggcc aaaatcccaa ccgaggtgct ggtcaccgat cgccgcgaat ttgaactggc
     2161 tgaagagggt tttatcaccc ttaccatgcg taaagactct gataatgcag cctttttctc
     2221 tgcaaactcg gtacaaaaac cgaagcactt tccgggaaaa gatgcagaaa ccaactataa
     2281 gctgggtacg cagcttcctt atctcttcat catcaatcgg ttagcgcact acatcaaggt
     2341 gttgcagcgt gaacaactgg ggtcatggaa agaaaggagc gacttagaaa gagagcttaa
     2401 tacctggatc cgtcagtatg ttgccgacca ggaaaacccg cctgcagacg tgcgcagccg
     2461 caaacctctg cgcgctgcca aagttgaggt tatggatgta gaaggcgaac ccggctggta
     2521 ccaggttgcg ctaagcgtga ggcctcattt taaatttatg ggggcaaatt ttgagctttc
     2581 cctggttggc cggttagaca gggagtaaca caccatgata ccggatcggg aaagaaccac
     2641 aggttctctt tttgagcgta tggaagcctc ttcggccaga aaccggcagg gaggcagtat
     2701 tcattcgctc aggcaatcca ttcgccagaa cctacgaaac attcttaata cccggtccgg
     2761 tagttgccgg ggtgcgcctg agctgggcat tgatgaaccg gaaggggctg aaaacttccg
     2821 cgaatccatg agtcgggcca tcgagcaatg tatagaacgc tatgaacccc ggatctcaca
     2881 cgctgaagtg caggcagtgg tttcatccgc ctccagcccg ctggatatga cgtttcatat
     2941 tacagcctgg gtgacattta acgaaacgca tgaggtgctt gaatttgata tggcaccgaa
     3001 tggcagccag cattaccggg tggactgaca aatggaattt gaagaacgct atttccggga
     3061 agaactcgat tacctgcgcc agcttagcaa gctgctggca acggaaaaac cccatctggc
     3121 ccgcttcctg gccgaaaaag atgcggatcc ggatattgaa cgcctgctgg aaggggtggc
     3181 ttttcttacc ggcaatctcc gccagaaaat tgaggatgaa ttcccagaac tgacgcacgg
     3241 gcttattaag atgctatggc ctaattacct gcgtccggtt ccggcaatga cccttattga
     3301 atatacgccg gatatggata agtcttctgt accggtgtta atcccccgta atgagcagtt
     3361 tacaaccaac gccggggaaa tcagagttga tgaagtgctg ccctctgatg ctaaaaagga
     3421 ggagccgcct ccctgtacct tcactctctg ccgggatatc tggctgctgc ccgttcgcct
     3481 ggagcagatt gaaaaccgca gtacgacccg taatggtgtt atcaacatca ccttttcggt
     3541 cgcaccggga acggacttcc gcacgctgga tctgaacaaa cttcgcttct ggctcggcaa
     3601 tgacgacaac tatacccgtg accagcttta tttatggttc tgcgaatact tgcagggtgc
     3661 cgacctgact gtgggtgaac agcatattcg cctgcctgag tttatgctaa aagctgtcgg
     3721 ttttgagccg caggatgcca tgctgccctg gccgaaaaac gtccacagcg gctaccggat
     3781 ccttcaggag tatttctgtt accccgatgc gtttctcttt tttgatcttt gtggttgtcc
     3841 ggctttgcct gacggattgc aggcggaatt ctttaccctg caactgcgtt ttacgcgccc
     3901 tttgcccgtg gacatccggc tgcgccgcga ttccctgcgc ctgtattgcg cacctgccat
     3961 taatttattt atccaccatg cagaagccat cacgctggac aaccggcggg cagactatcc
     4021 gctggttccc agccgccatt acccacaaca ttacgatgta ttttccgtta acagtgtggt
     4081 gagccaggtc caggatatgt tcaggaaaaa agatctgggg cgtcctgttt cgacgcaggc
     4141 cgcgcgccag tggccagcct ttgaaagttt cagccatcag atggaataca gccggaagcg
     4201 ggaagtggtg tactggcatc accggaccaa aacatccctg ttccatcgcg gctttgatca
     4261 tacccttgcc tttatacatg ctgatggcag ttatccgtca gacgaatctc tgctcagtaa
     4321 tgaagtggtt tcggtatcgc tgacctgtac caaccgtgag cttccgtcac aaattcgttc
     4381 cggcgatatc accggcacaa ccggtaaaaa tgcggctgtt gcttcatttc gcaacattac
     4441 ccgcccgacg caaccactct ggccggtcat tgatggcagc ctgcactggt ccctactctc
     4501 cgccatgaac ctgaattatc tgtcattact ggatacggac gcgctgaagc aggtcatcgc
     4561 caactttgat cgccacgcaa tccatcatcc gcagacggca cggctgtcac aacaaaagct
     4621 ggatgccatt gagcgtctgg agacccgccc cgttgatcgc ctgtttacgg gtattcccgt
     4681 ccggggactg gcctccacgc tgtatctgca cccggagccg tttgtctgtg aaggggaaat
     4741 gtatctgctc ggtacggtgc tttcgcattt tctgtcgctg tacgccagcg ttaactcatt
     4801 ccacatgctg accgttgtga acacagaaag ccaggagaca tggaaatgga cggaaagaat
     4861 cgggcagcat cctcttatct gagtccggga aatcccccgg cagataaaga gcagaatgat
     4921 ccgctggcac aggttttcca taatgcctgc tcttacaatt tttttgcgat ggcggagctg
     4981 ttgcaccgcc tggcaaaggg tgaaaagggg acgccagaat tatccctgcg tgacgatccg
     5041 gcacaggaaa ccctgcgttt cagcgccgat gccagccttg ccttcccgtg cagtgatatc
     5101 agtgcactga aacgggatac gtcaggggca ttcaggatga ccaccacctt tatggggtta
     5161 caggggagcc agtcgcctct ccccggttat tacctcgatc acctggcctg gaaagcggta
     5221 catgaacaaa gtccggtagg tgattttctg gatatgttca gccaccgcct gacccagttt
     5281 gtctggcata tctggcgtaa gtaccgctat cacatcagtt ttcgtaacgg cggtgtggat
     5341 gcgttctcgc agcgcatgta ctcccttgtc gggctgggcc accgccagct tcgcgataag
     5401 ctggcgatta atcacagcaa aatgctggcg tattccggga tcctggcaaa tccgggacga
     5461 tccccggaaa taatctgtgg cctggtgtcc cactgctttg atttgtcaga agtgacgctg
     5521 caaaactggc aacgtcgcaa ggtggatatt gaaccggatc agcaaaatag tctggggagc
     5581 tattctctta aaaatggcga aaaactggcg ggaagatcgg tactgggcaa ctttgttctg
     5641 ggtacacgag tgcccgatct cagcggaaaa ttccagctca gtattaccag cctgacccga
     5701 aaacagttcc tctcttttct gccgtccggc gaaaacttcc tgccactgac gatgttcgtg
     5761 tccttcatcc tgcgcgatca gcttgcctgg gacttacatc tggggctggc gccggagcag
     5821 gtgggcgcaa tgcgtctggg cgataacaaa agtgcgctgc tgggctggac cagcttcctc
     5881 ggcacaccgg aagaacgacc ctcagtcacc atcagggtac ggtcgtaaat tatgataatc
     5941 acggagcaga atatgccgga agagaaacta cagacgcttt cattgcaggt cattaacggc
     6001 agtgagctgg aaagcggacg ggcggcgcgc tgtctgttca cacagcaggg aaatgtcggc
     6061 catggccccg aatgccactg gtcggtacag gatcgtcagc agagcattcc ggcccaggct
     6121 tttaccgtta tcctgcacga tggcacattt tgtctgcgcc cgcagacggc acaactgtgg
     6181 ctgaatcagg caaaagtcac agcaacatca gacctaatac agttgcgcca gggcgatgag
     6241 atccagatcg gacggctgat ggtgagggtt catctgaacc ggggagatat tccccattac
     6301 gatgaggaaa tggccactcc cgaaaccatc gttaccaatc gcgatatgct cacggatacc
     6361 ctgctatcaa cggagggtac gccacactat ccgggaatga ctcaccggca ccaacttgca
     6421 gacaccgtgg taaacggttt ttctgccgat ccactccagg cacttcagtc cgaaagcctg
     6481 attaccacgg gcgatccgct ttcaggcatt gcggctgtcc ggccatcggc accgctgtcc
     6541 gatccggcaa gtaatggggg gatcaatact ccgtttatgg atctgccgcc catttatgcc
     6601 agccccggcg atcgtaatga cgacgtctct gcggcagaaa tggcgcaacg ccaccttgcg
     6661 gtcaccccct tactgcgcgg tcttggcggc tcgcttacca tgagcaattc cgacgatgcg
     6721 gatgattttc tggaggaggc cggacgaacg ttacaggccg caataaaagg tctgctcgat
     6781 ttgcagcagc agcgtaacag cctctcagac aaacatttgc gcccgctgga agataacccg
     6841 ctgcgcctga acatggatta cgccaccgcg ctcgacgtaa tgtttgccga aggtaaaagc
     6901 ccggtacatc tggcggctcc cgccgccgtc agtgaaagcc tgcgcaatgt ccgccaccac
     6961 gaagaagcta accgggcagc gattgtggag tcgcttcgtg tcctgctgga tgctttctca
     7021 ccacaaaatc tgctgcgccg ctttgtgcag taccgccgca gccatgaact gcgccagccg
     7081 ctggatgatg ccggagcatg gcaaatgtac agccattatt acgaagaact ggcctccgat
     7141 cgccagcagg ggtttgagat gctgtttaac gaggtctacg cccaggtcta tgaccgggtg
     7201 cttcgtgaaa aacagcggga gccggaagca tgaacaggaa ggcgtttctg gcctgcgtgc
     7261 tgatgtgcgt catattaacc ggctgcgaaa cggcgaaaaa aatcagtgag gtgatcaaaa
     7321 acccggatat ccaggtcggg agcctgaagg agcaaccttc ggaaataacc gtcaccctgc
     7381 tgaccgaacc ggacaccaat accaatgcgg aaggtgaatc agccgccgtg gatgtgcaac
     7441 tggtgtacct gactgacgac tcaaaattgc tggcagccga ctatgaccag atagccagta
     7501 cgccgctacc ggatgtgctg ggtaaaaact atatcgacca ccaggatttc aacctgttgc
     7561 cggacaccat caaaaccctg ccgccggtaa agctggatga gaaaacgcag tttattggtg
     7621 tcgtggccta tttctccgat gaccaggcca ctgaatggaa acaaatcgag acggtggaag
     7681 gaaccggcca ccattaccgc ctgctggtgc atgtacgtca aagcagcatc gaaatgaaaa
     7741 aagaggatga ataattatgg caaccacccg caacaaggtg atgtggcagg aagggatgct
     7801 gatgcgccca caccatttcc agcaacagca gcgttacaac gactacctgg ataaccagcg
     7861 tttccgggcc atgaatgatt tatcctgggg atttaccgaa ctcaccctca acaatgaact
     7921 gctggcgcag ggtaagatca tgattgacag cgcgtcaggc acactgcccg acggcaccgt
     7981 cttttctatc cccgaccagg acgcactgcc cgatccgctg cacccgcaac attttccgga
     8041 cgagagaagc cgcaatatct acctcgctct gccggtcgcc agtgatgtga gaaatgaaat
     8101 cagcgacggg cggcgaatcg ggcgttaccg gctgaattat gccgatgtcc gggatttgca
     8161 ttcagaagaa ggtgacacgc gaacactgac gctgggacaa ctgacgccgc gcattatgag
     8221 cggtgcagaa gatatgagcg cctatattac gctcccgctt tgccgtatca gtgatcgcca
     8281 tgccgacggc tccctgacgc tggatgacga ttttatcccc tcctgccaga atattcaggt
     8341 cagtaagaaa ctgcgtgttt atctcaaaga ggtacagggg gccattggcg gacgggcaag
     8401 cgatctggca aaccgcattg gctctccggc gcagagcggc atcgcggatg tggcggaatt
     8461 tatgatgttg cagttactta accgtaacca gacccggttt acccatcgcg ctcgtcgatc
     8521 ccagctccac ccggaagatt tctaccttga tcttgccggg ttgctgggtg aactgatgac
     8581 ctttaccgag ccgtcgcgcc tgccctgccc gcttgatgtg tatgatcatc atgacctgac
     8641 caaaacattt aaaacgctgt taccggaagt caaacgggcg ctgcataccg tactgtcgcc
     8701 aagagccgtt aatctgccgc tgcatctgcg tgacggtatc tggcaggccg atatccatga
     8761 cacagaactg ctgcaatctg ccacctttgt gctggctgtg gcggcaaaca tgccggtcga
     8821 tcagatccag cgccagttta tccagcagtc gaaaatttcc tcgccggaaa aaatccgcaa
     8881 tatggtcagt gtgcagatac ccggcattcc attgcgtgcc ctgatggtgg ctccccgcca
     8941 gcttccttac cattccgggt tcagctattt cgaactcgac aagagcggac aggcctggac
     9001 agaaatggct gccgccgggg ccgttgcact gcatgtatcc ggcagtttcc cggatctgaa
     9061 catgcaactg tgggcgataa gagggtaaga cgatggatga ggggtcactg tctctgccac
     9121 catttaccgg atacgacgaa aaaagccagc gcgactatca tctcgcgctt cgtggcaaca
     9181 gcctgaatcc gatgatcgac gcggccacgc cgctactcgg tatggtaatg cgcctttcaa
     9241 ccatgaacag ccagacgatg ccggagcatc tttttgctca ggtggtgacg gatgttcagg
     9301 ccgtggagca actgcttcag gagcagggct acgaaccggg cgtgattatc tcgtttcgct
     9361 acatcctctg cacgtttatt gatgaagcgg cgctcggtaa cggctggtcg aacaagaacg
     9421 agtggatcaa gcagtcattg ctggtccatt tccacaacga agcctggggt ggagagaaag
     9481 ttttcatcct gctggagcgc ctgatccgtg aacccaaacg ttaccaggat ctgctggaat
     9541 ttctctggct ctgtttttct ctgggatttc gcgggcgcta caaagtggca gcacaggatc
     9601 agggggaatt tgagcagatc taccgccgcc tgtaccacgt cctgcataaa ctacgcggcg
     9661 atgccccttt cccgctgtta caccaggaca aaaaaaccca gggcgggcgt tatcagctca
     9721 tcagtcgcct gaccattaaa catatttttt gtggtggcgt tgtcgtgctg gcgttgtttt
     9781 acctgttcta tctgttgcgg ctggacagcc agacgcagga catcctgcac cagctaaaca
     9841 aattacttcg ataaggacgt ttatgatcca gattgatctt cccacgctgg taaaacggct
     9901 taacctgttc tcccgccagg cgctggagat ggccgcctct gaatgtatga gccagcaggc
     9961 agcggaaatt accgtcagcc atgtgcttat tcagatgctc gccatgccac gcagtgacct
    10021 gcgggttatt acccgccagg gcgatattgg catggaagag ttgcgccagg cgctgacggt
    10081 ggagaactac acaactgccc gttctgcgga cagctacccg gcattttccc cgatgctggt
    10141 tgagtggcag aaagagggct ggctgctggc gtcggctgag atgcagcaca gcgaattgcg
    10201 tggcggcgtg ttgctgctgg ccctgctgca ttcgccgctg cgttatatac cgcctgctgc
    10261 cgcccggctg ttgaccggca ttaaccgtga ccgtctgcaa caggactttg tgcaatggac
    10321 acaggagtcg gcggaatcag tcgtgccgga tgcagacggt aaaggcgcag gcacactgac
    10381 ggacgctgct gacaccctgc ttgcccgcta cgccaaaaac atgaccgcag acgcccgtaa
    10441 cggcaggctt gacccggtac tgtgccgcga ccacgaaatc gacctgatga tcgacattct
    10501 ctgccgccgc cgcaagaaca acccggtggt ggtgggcgaa gcaggcgtgg gtaaaagcgc
    10561 cttaatcgaa gggctggcgc tgcgcatcgt ggcaggccat gtgccggaca agctgaaaaa
    10621 caccgatatc atgacccttg acttgggcgc attgcaggcc ggagcgtcgg tgaagggtga
    10681 attcgaaaaa cgtttcaaag ggctgatggc ggaggtcatt tcctccccgg tgccggtcat
    10741 tctgtttatc gacgaagcac ataccctgat tggcgcgggc aaccagcagg gcgggctgga
    10801 tatctccaac ctgctcaaac cggcgctggc gcgcggcgag ctgaaaacca tcgccgccac
    10861 cacctggagc gagtacaaaa aatacttcga aaaagatgcc gccctgtcgc gccgcttcca
    10921 gttggtgaag gtcagcgaac ccaacgctgc cgaagccacc attattctgc gcggtctgtc
    10981 ggcggtctat gaacagtctc acggcgtgct gattgatgat gacgccttgc aggccgctgc
    11041 gacattaagc gagcgttatc tctccgggcg tcagttaccg gacaaagcga ttgatgtgct
    11101 ggataccgcc tgcgcccgtg tggccatcaa cctgtcgtcg ccgccgaagc aaatctcggc
    11161 gctgaccact ctgagccacc agcaggaggc ggaaattcgc cagcttgagc gcgagcttcg
    11221 catcggactg cgtaccgaca catcacggat gaccgaggtg ctggtgcagt atgatgaaac
    11281 gctgacggcg ctggatgaac tggaagcggc ctggcaccag cagcagacgc tggtccggga
    11341 gattattgcg ctgcgccagc agttactggg cgtggcagag gacgatgcgg cgccgttgcc
    11401 ggacgcagat accgtggagg atacgcagcc agagtcagag tcagagtcag agtcagagtc
    11461 agagcaggat aataccggtg ccgaaccggc tgatgaagct ggcagcgaac agccggaaga
    11521 gaccgctgaa acagtttccc cggtacaacg tctggcacat ctcactgccg aactggacgc
    11581 cctgcataac gaccggttgc tggtttcccc gcacgtcgat aaaaaacaga ttgcggcggt
    11641 gattgccgaa tggaccggcg tgccgcttaa ccgcctgtcg cagaatgaaa tgtcggtcat
    11701 caccgacctg ccaaaatggc tcggcgacac catcaaaggc caggacctgg cgattgccag
    11761 cctgcacaaa cacctgctga ccgcacgcgc cgacctgcgc cgtccgggac gcccgctggg
    11821 cgcgttcctg ctggctggcc ccagcggcgt gggtaaaact gaaaccgtcc tgcaactggc
    11881 agagctgctc tacggcggtc gccagtacct gaccaccatc aatatgtccg agttccagga
    11941 gaaacatacc gtctcgcgac tgattggttc gcctccgggc tacgttggct acggtgaagg
    12001 cggcgtactg accgaagcga ttcgccagaa accctactcg gtagtactgc tcgatgaagt
    12061 ggaaaaagcg cacccggatg tgctcaacct gttctaccag gcgttcgata agggcgaaat
    12121 ggcagacggt gaaggccgcc tgattgactg caaaaatatc gtcttcttcc tgacgtccaa
    12181 cctcggctac caggtaatag tcgagcatgc cgatgacccg gaaaccatgc aggaagtact
    12241 gtatccggtg ctggccgact tcttcaaacc tgccctgctg gcgcgtatgg aagtggtgcc
    12301 gtacctgccg ctgtcgaaag agacgctcgc caccatcatt gccgggaaac tggcccgcct
    12361 ggataacgtg ctgcgcagcc gctttggcgc ggaggtgatt atagaaccgg aagtgacgga
    12421 cgaaatcatg agccgcgtca cccgcgcgga aaacggcgcg aggatgctgg aatcggtcat
    12481 cgacggcaat atgctgccgc cgctctccct gctgctgttg cagaaaatgg cggcgaatac
    12541 ggcggttgcc cggattcggt tgtcggcagt ggacggcgca tttacggcag acgtggaaga
    12601 tgctcagaac gacgagtccg tcacaaagga tgaaacggtt ttatgagcag gttgcgcgtt
    12661 ttccggcaga caggatggtt tatcgcgggc ctgatgacgg gcttacccgc cactgccgcg
    12721 cccgcagaag catcgtcaat ggccggtgtg gcggtcgccg tcgccaccac gaccccgccg
    12781 gatgcgacag ccacgttaca ggccatgcag tcctgtcggc gggagtccgc ggcgctggag
    12841 cggcttgact gctatgaccg cctgctggca ccgctgtccc cgtcagggtt tgacggggcg
    12901 ctggtcaaag ccggttttgt gggcgaagcc tggacccgcg ccacggaaca ggaaaaacac
    12961 cgccagggca acaccacaga gctgctggtg acgcaggtgc cgggggagcg cccgacggtg
    13021 gtgataacca ccccggctat cggtcatgtt ccgccacgcc cggtgctgat gttcagttgc
    13081 gtggacaata tcacccggat gcaggtggcg ctgatgcacc ctctggatgt ccatgatatc
    13141 gccgtcactc tgaatgccga caaccgggca ttgcgcagcc actggtttgt gcgcgagaac
    13201 ggcaccctgc tggagtccag ccgggggtta tccgggattg atgaaattaa gcaactgttc
    13261 ggcgcaaaaa cgctgacggt ggataccggc gcagacaatg ccgccggaaa gctgaccttt
    13321 aacattgacg ggctggcaag ggcgattgct cccctgcgcg atgcctgcca ctgggcggga
    13381 gaataaaaat atggcaatgg acttacgcga tccgaatgtc tggatatcgc acctgctgga
    13441 aaacctgccg gaagagaaac tggcatcggc gctgaaagat gacaacccga actgggagta
    13501 tatcgacggc gaaatcgtca agctgggatc gctggcccac agtcaactgg atatcccgga
    13561 actccagcgc cgggggctgg tcattctggc ttcggagagt aaggattttc gtctgctggc
    13621 gcatctgctg agaaccctgc aacacgccgg tgatcctcat ctcgcattgc gcctgctggc
    13681 gctgtatgtg gagcattact gggcggtggc tgcgccgcag aatatggcgc ataaaaaacg
    13741 ctttgccagc caggtcatta aacgctttga aacaggtatt gaagtttttt cacagaacgc
    13801 cgccacagcg cagcgcgatg cgctgtcagg ggaactggca aaactggcgc aatgctggca
    13861 gtcacataac gccccggaac tggcacaggc tactgatgat ctttttgccc tgtaccagcg
    13921 tgcgtttaat cgtgcggcac cggctcctgt ccccactccg gcggcctccg gtagttcacc
    13981 acagaccacc gccacgtctg aaagcggcgt gacgcaaccc agtgctccgg ctccccaaat
    14041 cgtcatcgac agtcacgacg acaaagcctg gcgcgacacg ctgttaaaag tggcggccat
    14101 tttatgtgaa cgccagccgg attcaccgca gggctaccgt ctgcgccgcc atgcgctttg
    14161 gcagaacatc accagtacgc cccaggcgga aagcgatggc cgtactccac tggctgcggt
    14221 ctccgccgat atggtggcag attaccatgc tcagctcggt agtgccgata tggcgctgtg
    14281 gcagcaggtg gagaaaagcg tactgctggc tccttactgg ctggatggtc actgcctttc
    14341 tgcacagacg gcgctgcgtc tgggttacaa acaggtggca gacgccatcc gcgatgaggt
    14401 catccgcttc cttgagcgtc tgccccagct taccgggctg ctgtttaatg accacacacc
    14461 gtttatcagt gagcagacga aacaatggct ggcagcttcg cccgacgcca aagttgcacc
    14521 ggttgcgcaa atcggtgagg aatcgaaggc agccagagcc tgttttgctg agcagggtct
    14581 ggaggcggcg ctgcgatatc tggacatgct acccgaaggc gatccccgcg atcagtttca
    14641 ccgccagtac cttgccgcac agttgacgga agaggcgggg ctggtacagc ttgcgcagca
    14701 gcagtaccgg atgttgttca ggatgggatt gcagatgatg gtggcagact gggagccgtc
    14761 cctgcttgag cagctagaac aaaaattcac ggcagaacaa taaaagagaa caggagtttt
    14821 tgtgttcaga ttaccaacac cccgactact cagcggactc aaatcagccc tgcgaccggc
    14881 gatgccccgg tttaaagtct ctgctttctg gctgctgata ctggcgtgga tttttctgct
    14941 ggtgtggatc tggtggaaag gcccgacgtg gacgctgtat gaagaacagt ggctcaaacc
    15001 actggcgaac cgctggctgg caacggcggc gtgggggatt attgccctga tgtggcttac
    15061 cgtccgggtg atgaagcgcc tgcaacagct tgaaaaaatg caaaagcaac agcgcgagga
    15121 agccgttgat ccgctcagcg tggaactgaa cgcccagcag cgttatcttg accgctggct
    15181 gctgcgcctg caacgccatc tcgacaaccg ccgtttcctg tggcagttgc cgtggtatat
    15241 ggtcatcggc ccggcgggca gtggcaaaac gacactgctg cgcgaggggt ttccgtccga
    15301 cattatttat gccccggagg gcgcacgcgg cgcagaacaa cgcctgtacc tcacgcccca
    15361 tgtcggtaaa caggcggtga tctttgatat cgacggcaca ctctgcgctc ccgctgatgc
    15421 ggatatcctg catcgccgtc tgtgggaaca tgccctcggc tggctgaaag aaaagcgcgc
    15481 gcgccagccg ctgaatggga ttattctgac actcgattta cccgatctgc ttaccgcaga
    15541 caaacgccgc cgcgagcatc tgttacaggc gctgcgcagc cgcttgcagg atatacgcca
    15601 gcatcttcac tgccagttac cggtttacgt ggtacttacc cggcttgatt tattgcaggg
    15661 ttttgccgcc ctgtttcagt ccctgaacag acaggatcgc gatgcgattc tgggcgtcac
    15721 gttcacccgc cgtgcccatg aaaatgatga ctggcgaaca gagttgaatg ctttctggca
    15781 gacatgggtg gatcgaatga atctggcgtt gccggatctg atggtcgctc agactcacac
    15841 ccgcgcgtct ttattcagtt tttcccgcca gatgcaggga agccgtgaac cgctggtgtc
    15901 actgcttgag ggtctgcttg atggcgaaaa tatgaacgtg atgctgcgtg gtgtctatct
    15961 cacctcttcg cttcagcgtg gacagatgga tgatatattc acccagtctg ccgcccgcca
    16021 gtaccggctg ggcaataacc cactggcgtc ctggcccctg gtggacaccg cgccttattt
    16081 cacccgcagc ctgttcccgc aggcattact cgcagagcct aatctggcaa cagagagccg
    16141 cgcctggctg atacgttccc gtcgccgcct gacggttttc tctgccacag gcggcgtggc
    16201 agcactgctg ctcatcaccg gctggcatca ctattacaac ggtaactatc agtctggcat
    16261 caccgtgctt aagcaggcca aagcctttat ggacgtgccg cctccgcagg gggaagatga
    16321 ctttggcaac ctgcaactgc cgctcctgaa cccggtacgc gatgccacac tggcctatgg
    16381 cgactggggc gaccgcagcc gtctggccga tatgggactg taccagggac gacgtatcgg
    16441 gccttatgtg gaacagacct atctgcaact gctggagcaa cgttacctgc cctcgctgtt
    16501 taacgggctg gtcaaagagc tgaacgccgc gccgccggag agtgaagaaa aactcgcggt
    16561 gctgcgcgtg atgcgaatgc tggaggacaa aagcggacgt aacaatcagg tggtgaagca
    16621 gtatatggca aaacgctgga gcgaaaagtt ccacggtcag cgcgatatcc aggcacaact
    16681 gatgtcccat cttgactacg cgctggctca tactgactgg cacgcagagc gtcaggcggg
    16741 cgacggtgac gccatcagcc gctggacgcc atatgacaag cccgtggtat cagcacagaa
    16801 agaactgagc aaactgcctg tctaccagcg ggtttaccag agcctgaaaa cgcgggcgct
    16861 gggcgttctt cctgccgacc tcaatctgcg tgaccaggta gggccaacct ttgaccaggt
    16921 gtttacatct gccgatgaca acaaactggt tgttccacag tttcttaccc gttacggcct
    16981 gcaaagctat tttgtaaaac agcgcgatga actggttgaa ctgacggcga tggattcctg
    17041 ggtacttaac cttacccgca gcgtgaaata cagtgacgcc gaccgcgcgg aaatccagcg
    17101 ccagttgacc gagcagtata tcagcgaata caccgccacc tggcgggccg ggatggacaa
    17161 tctgaatatc cgcaattttg agtccatcgg acaactgacc ggggcgctgg agcaggttat
    17221 cagcggcgac cagcctttgc agcgggcgct aaccgtgccg cgtgacaaca cacagccagg
    17281 cgtcttttct gaaaaactct ctgccaaaga acgggaggaa gccctggcag agccggatta
    17341 ccagttactc acccgcctcg ggcatgaatt cgccccggaa aacagtaccc tggcagtaca
    17401 gaaagacaaa gaaagcacga tgcaggccgt gtatcagcaa ctcaccgagt tgcaccgcta
    17461 cctgctggca atccagaacg cgcctgtacc aggaaaatcg gcgctgaaag ccgtgcagtt
    17521 acggcttgat cagaacagca gcgatccgat attcgccacc cgccagatgg caaaaacgct
    17581 gcctgctccg ctcaaccgct gggttggcag gctggctgac caggcctggc atgtggtgat
    17641 ggtggaggct gttcattata tggaagtgga ctggcgcgac agcgtggtga aaccgtttaa
    17701 cgagcaactg gcaaataact atccgtttaa tccgcgttct gcacaggatg cctcactgga
    17761 tgccttcgaa cgcttcttta aaccggatgg catactggat accttctacc agcagaacct
    17821 gaagctgttt atcgataatg acctgagtct ggaggatggc gataacaacg tcattattcg
    17881 cgaagatatt attgcgcaac tggaaactgc gcagaaaatc cgtgacatct tcttcagcaa
    17941 acagaacggt ctgggaacat cctttgccgt ggaaacggta tcgctttcag gcaataaacg
    18001 ccgcagtgta ctgaaccttg acggtcagtt agtcgattac agccagggcc gtaactatac
    18061 cgcccatctg gtctggccta acaacatgcg cgaaggcaac gaaagtaagc tgacgctcat
    18121 cggcaccagc ggcaacgcgc cgcgcagtat cagcttcagc gggccgtggg cgcagttccg
    18181 cctgttcggg gccggacaac tgaccggagt acaggatggc aactttaccg tgcgctttag
    18241 cgtggacggt ggcgcgatga cctaccgtgt gcataccgac acggaagata acccgttcag
    18301 cggtgggttg ttcagccagt ttggtctgtc agacacactg tactgaggag gcagggatga
    18361 atagtaacgt actgacacaa actatcgtta ccggcagtga cccgcgcggg ctgccggaat
    18421 tcagcgccat ccgcgaggaa ataaacaaag ccagccaccc gtcacagcct gagctgaact
    18481 ggaaactggt ggagtcgctg gcgctggcga tttttaaagc caacggtgtg gatttacaca
    18541 ccgccaccta ctatacgctt gcccggacac ggacccaggg actggcggga ttctgcgaag
    18601 gtgcggaact gctggcggca atggtaagcc acgactggga taagttctgg ccgcagggcg
    18661 gcccggcgcg tactgaaatg ctggactggt ttaactcccg aaccggcaat attctgcgtc
    18721 agcaaatctc ctttgcggaa tccgacctac cactgatcta ccgcacagag cgggcattgc
    18781 agcttatctg cgataagctc cagcaggtgg aactgaagcg cgttccgcgc gtggagaatc
    18841 tgctctattt tatgcagaac acgcgtaaac ggcttgaacc ccagctgaag agtaacactg
    18901 agaacgccgc acagaccacg gtcagaacgc tgatttatgt cccggaaaca caggcatctt
    18961 ccacaccaga agcggtagtg cctcccctgc ccggcctacc tgagatgaaa gtggaagtgc
    19021 gcagtctgac agagaatccc ccacaggcca gtgtgataaa gcaaggcagt acggtaagag
    19081 ggtttatcgc agggatcgcc tgttcagtgg ctgtcgcctc agcattgtgg tggtggcagg
    19141 tctatccggt gcagcagcaa ctgttacagg ttaacgacac cgctcagggc gcagcaacgg
    19201 tgtggatggc ctcacctgaa ctcgaaaact atgagcgcag gctgcaacaa cttcttgata
    19261 cctccccggt acagccgctg gaaaccggga tgcagatgat gcgtgttgcc gacagtcgct
    19321 ggccggaaag cctgcaacag caacaggcct cgacacaatg gaatgaggca ctcaaaaccc
    19381 gcgcacagag tagcccgcag ttgcgtggct ggttgcagac ccgccaggac ttacatgctt
    19441 ttgcagatct agtgatgcag cgcgagaaag agggactaac cctttcctat atcaaaaatg
    19501 tcatctggca ggcggagcgg ggactggggc aggaaacacc cgttgagtct ctgttgacgc
    19561 agtaccatga tgcccgtgcg cagaagcaga atacagatgc gctggaaaaa caaattaatg
    19621 agcgactcga aggcgtgtta agccgctggc tgctgctgaa gaataacgtc atgccagagg
    19681 cggcaaccgg taccacggcc gaaaaataat tatttaatta aaggagatta tcatggcaaa
    19741 tataagttat ttatctttga gtggagagac acaagggcta atttcagcag gttgctcaac
    19801 gcttgattct gtcggaaaca aagcgcagcc agaacataaa gatcagatca tggtttatgc
    19861 gttaatgcat tccatctcaa gaagtcaaaa tgtcaatcat catgaattaa ttatcaccaa
    19921 accggttgat aaatcctcac cattgctggc aaaagcactt agtgacaatg aaaaaatggc
    19981 aatatgtgaa tttatactct accgaaccag taaagcgggt atttaccagc cttattataa
    20041 aataaatctg tcaaaagccc gaatatcaag tattgatttt gtcacaccgc atgctgtcct
    20101 ggaaaaagaa ctggaaccac aggagagaat tgcctttatt tatgaagata tttcctggga
    20161 gcatacgtta gccggtacga atgcaatgag taaatggcag gatcgtgtac agtaacttac
    20221 tgtaataaca taataaatta ctattgcgga gagataaaat ggctggttta aggaataaca
    20281 acaacacaca gaatgcacag tgggctgatt atgtgggtga cattcttcgg ggggctcagc
    20341 cgataaacca gctagtacct cagcaccctt atcttaacga cgtcccactc attgatgagt
    20401 taaggcatca gaacactcat catgttattc tcttgacact tgatgttgca aaaaaaatac
    20461 tcagcccaat aacatcattt gattacattc attttatcac tacacatcct tcaggtataa
    20521 aagatacact cgcctggtta gttaacgcga gtaaattaat gactgagttt gatgacaatg
    20581 ggaaaattat atttaacctt aatgccttaa agtatacaaa agcaagttac tttgaaatac
    20641 ttggagagaa atatataaaa atcacaacat ccagcccctg gcttttagaa aagctcggta
    20701 aatatatttt ctcttcacga gcgcctcaag ttctggaact cgctattggc tggcgaggag
    20761 ctttatctga atcaataaaa ggagttaagt tctgtatctg gttttcagta gcctggagaa
    20821 ccatagaatt tattatgagt tcagagcgag atctggttaa ttttcttggc gatttttcaa
    20881 tggatgtggc caaagcagtt atagccggtg gtgttgcaac cgctattgga agcctggctt
    20941 cttttgcctg tgttagcttt ggctttccag taattcttgt cggaggagca attttactga
    21001 cagggatagt gtgtacagtt gttttaaatg aaatcgatgc tcaatgccat ttatcagaaa
    21061 aattaaaat
//
LOCUS       DAZGXZ010000089        20855 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000089 DAZGXZ010000000
VERSION     DAZGXZ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.089"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..787
                     /locus_tag="ACHKJF_003407"
                     /pseudo
     CDS             2..787
                     /locus_tag="ACHKJF_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(791..2182)
                     /gene="tcyP"
                     /locus_tag="ACHKJF_003408"
     CDS             complement(791..2182)
                     /gene="tcyP"
                     /locus_tag="ACHKJF_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP0950711.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2315..2905)
                     /gene="ydjM"
                     /locus_tag="ACHKJF_003409"
     CDS             complement(2315..2905)
                     /gene="ydjM"
                     /locus_tag="ACHKJF_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0950712.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3068..3736)
                     /gene="hxpB"
                     /locus_tag="ACHKJF_003410"
     CDS             complement(3068..3736)
                     /gene="hxpB"
                     /locus_tag="ACHKJF_003410"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP0950713.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3883..4419
                     /gene="yniB"
                     /locus_tag="ACHKJF_003411"
     CDS             3883..4419
                     /gene="yniB"
                     /locus_tag="ACHKJF_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP0950714.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4460..5320)
                     /gene="yniA"
                     /locus_tag="ACHKJF_003412"
     CDS             complement(4460..5320)
                     /gene="yniA"
                     /locus_tag="ACHKJF_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP0950715.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5426..5716)
                     /gene="ghoS"
                     /locus_tag="ACHKJF_003413"
     CDS             complement(5426..5716)
                     /gene="ghoS"
                     /locus_tag="ACHKJF_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0950716.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5817..6746)
                     /gene="pfkB"
                     /locus_tag="ACHKJF_003414"
     CDS             complement(5817..6746)
                     /gene="pfkB"
                     /locus_tag="ACHKJF_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP0950717.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7033..7791
                     /gene="ydiY"
                     /locus_tag="ACHKJF_003415"
     CDS             7033..7791
                     /gene="ydiY"
                     /locus_tag="ACHKJF_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP0950718.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7844..7951)
                     /gene="yniD"
                     /locus_tag="ACHKJF_003416"
     CDS             complement(7844..7951)
                     /gene="yniD"
                     /locus_tag="ACHKJF_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP0950719.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(8127..10025)
                     /gene="espL1"
                     /locus_tag="ACHKJF_003417"
                     /pseudo
     CDS             complement(8127..10025)
                     /gene="espL1"
                     /locus_tag="ACHKJF_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10548..12476
                     /gene="thrS"
                     /locus_tag="ACHKJF_003418"
     CDS             10548..12476
                     /gene="thrS"
                     /locus_tag="ACHKJF_003418"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP0950720.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12480..13022
                     /gene="infC"
                     /locus_tag="ACHKJF_003419"
     CDS             12480..13022
                     /gene="infC"
                     /locus_tag="ACHKJF_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP0950721.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            13119..13316
                     /gene="rpmI"
                     /locus_tag="ACHKJF_003420"
     CDS             13119..13316
                     /gene="rpmI"
                     /locus_tag="ACHKJF_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP0950722.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            13369..13725
                     /gene="rplT"
                     /locus_tag="ACHKJF_003421"
     CDS             13369..13725
                     /gene="rplT"
                     /locus_tag="ACHKJF_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP0950723.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13842..13966
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13848..13892
                     /gene="pheM"
                     /locus_tag="ACHKJF_003422"
     CDS             13848..13892
                     /gene="pheM"
                     /locus_tag="ACHKJF_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP0950724.1"
                     /translation="MNAAIFRFFFYFST"
     gene            14176..15159
                     /gene="pheS"
                     /locus_tag="ACHKJF_003423"
     CDS             14176..15159
                     /gene="pheS"
                     /locus_tag="ACHKJF_003423"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP0950725.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            15174..17561
                     /gene="pheT"
                     /locus_tag="ACHKJF_003424"
     CDS             15174..17561
                     /gene="pheT"
                     /locus_tag="ACHKJF_003424"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP0950726.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17566..17865
                     /gene="ihfA"
                     /locus_tag="ACHKJF_003425"
     CDS             17566..17865
                     /gene="ihfA"
                     /locus_tag="ACHKJF_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP0950727.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17966..18946
                     /gene="btuC"
                     /locus_tag="ACHKJF_003426"
     CDS             17966..18946
                     /gene="btuC"
                     /locus_tag="ACHKJF_003426"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP0950728.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            19009..19560
                     /gene="btuE"
                     /locus_tag="ACHKJF_003427"
     CDS             19009..19560
                     /gene="btuE"
                     /locus_tag="ACHKJF_003427"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP0950729.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19560..20309
                     /gene="btuD"
                     /locus_tag="ACHKJF_003428"
     CDS             19560..20309
                     /gene="btuD"
                     /locus_tag="ACHKJF_003428"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP0950730.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            20387..20851
                     /gene="nlpC"
                     /locus_tag="ACHKJF_003429"
     CDS             20387..20851
                     /gene="nlpC"
                     /locus_tag="ACHKJF_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0950731.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
BASE COUNT         5407 a         4820 c         5288 g         5340 t
ORIGIN      
        1 aatacagagt aattatggtg atttaaatat taaaagcctg gctttcgact cttttaaaga
       61 aaggcttgag tctaccatgg cggcactcac tttctttata tcaacgggtc aatgtgactg
      121 cgatgaaata gcagagtcga acttcaatcg tatgattgcc tatatgtcta acattaacta
      181 tgatgcctca aagccaggag cgccagcact tagttttgat acgtatctgc aggacaatgt
      241 taaatataga gtgataatta ataatcttta tggcagtgag tttcggataa gagatattaa
      301 tgagaatttt gttgggatgg attttacctg tgaattctga cccgaaaaaa tgacgaattt
      361 gatcaatata aaaataggtt ggttattcat tatttaagga ctatatacta tgaaaaaaat
      421 tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa
      481 gtttccttcg ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc
      541 gggaatggga agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cacttagaaa
      601 acgtctcggg tatgaggttt taagcttctc actacaggca cattcattga gccaggaatg
      661 tattgataag ttggacattt ttttcgctga cgatctgttt aaatatgagt cagtatgcat
      721 cgccgcgatg gaacatttaa aaagtaaagc aacggcccct atacaaaacg gaccgttgcc
      781 agcataagaa ttaacggtgt gccagttcgg cgtcgtcttc gctatccaga atggctttat
      841 cggtttgctt cagccactgg ctggtcagcg tgccagctgt catcgagcca ctaacgttta
      901 gcgccgtacg gcccatgtcg ataagcggtt caacggagat taacagcgcc accagggtta
      961 ctggcaggcc catcgcaggc agcacaatca gtgcggcgaa agttgcacca ccaccgacac
     1021 cggcaacgcc tgcggaacta acggtaacaa taccgaccag cgtcgcaatc cacatcgggt
     1081 ccagcgggtt aatgccaacc gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac
     1141 cggcgcagcc gttctgacca atggttgcac cgaaagaggc ggcgaaactg gcgatggatt
     1201 caggaacgcc cagacgacgc gtttgtgctt ccacattcag tgggatagac gcagcactgg
     1261 aacggctggt aaaggcaaac gtcagcacag gccatacctt acggaagtac ttcagcggac
     1321 tcacgccatt aatgcccagc agaatgccat gcactgcaaa cataatcagc agaccgaggt
     1381 aggacgcgac aacgaaactt cccagtttga tgatgtcttg caagttagaa cctgcaacca
     1441 ctttggtcat tagagccaga acgccgtaag gggtcaattg catgacaagg cgaaccagtt
     1501 tcatcaccca gctttgtagg gtatcgatag cggctaagac gcgttcacct ttcggcgcat
     1561 catccttcag cagtttcaat gcagctacgc cgaggaatgc ggcaaaaatt accacgctga
     1621 taattgacgt cggattggct ccggtaagat cggcaaacgg gtttttcggg ataaaggaca
     1681 agaccagctg cggaacgctc agatcagaga ctttaccaac atagttactt tcaatggcgt
     1741 tcagacgtgc agtttctgca ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt
     1801 tggtgaccag cacaccgacc agcgccgcaa tcagcgtggt aaacaaaagc gtaccgatgg
     1861 tcagaaaact gattttgcct aactgagatg cgttatgcag acgggcaacc gcgctcagaa
     1921 tagaggcgaa gactaacggc ataacgatca tttgcagcag ttgaacatag ccgttaccaa
     1981 cgatgttaaa ccactgtaca gaatctttaa gtacctggct gtcagaacca taaatggtat
     2041 gcagggcaag gccaaaaacc acacccatca ccagacccac cagcactttt ttcgccagac
     2101 tccactgttt atggcgggtc tgagccagcg caaacagcag tacaacgaac accacgatgt
     2161 tcgcaattaa tggaaagttc atccccgttc tcctgatctt attatttcta tcactgcgta
     2221 gcagtcatag tgttggcgca agattaacag aactgtgagc tggctcttat attcaaatgg
     2281 aattgcttat tccagaaagg tgatttttgt ctttttagta ttcaacctga tgcttaataa
     2341 gccgatgaaa ctggatctgc aaggtattga tcatttgcga tgaccaacga acagcgctgt
     2401 tctcgggtaa tgaatggggc atccataccg accagacaaa taatgccatg cagataaaac
     2461 gttccagttg gttgcccttt tggggaacca ggataggcaa gcggaaacgc cagcggcatg
     2521 gccagagcag gggaacaccg gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac
     2581 ccagcaccat tccttgtagc gcatcagccg gaatgaacca gccttccgga accttaaggt
     2641 aaaaggttgc cagcagcgca aataccgcca gcagactgtg ggtaaaacca cgatgcccaa
     2701 aagcgcgggc gatcggtttt gatatccatt ttaatcgctg cccaagaaac gactttgggt
     2761 gatcgatgtc cggtaacaaa cacgtcagga ttgcggaagg gacaatatgc caccagtcac
     2821 cctgtgccag cacgggcgtc agctcggcat ttttggcaaa taccgcacaa gcaatagaaa
     2881 agagaaggtg accttccgcc gtcatgataa aactcactaa actgtcgata cgtacagtat
     2941 aggattttta tacagtgcac ggaagtggtg acggtgtaac tctttctctt aaattgacga
     3001 ttaatccgtc agttgaatgc aaattcagat aaagaaccag gggcagcgag atcactgccc
     3061 ctggtcatta accgagaagg tcttttgcgg tgagttctgt cagcgatgag agtttgacgt
     3121 ttgctaatac aaaacgtgga tcattttgcg cttctggcgc aggaacgaca atggaacgca
     3181 tgcgggctgc tttagaggcg atcatgccat ttactgaatc ttccagcgct acgcaggtca
     3241 gtgggtcaac gcccagtttt gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt
     3301 aaggcagttt ttcagccgag gcgagggcat cgaaactgtc gcgtaagtca aacatggtca
     3361 acactttttc cagcatatgt agtggtgacg cggaggccag tcccaccaat aaaccttgtt
     3421 ctttgcataa cgcaacggct tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca
     3481 gtgaaatggc acgggcaata acccgttcta ctacttcctg acggcttggc ccattccacg
     3541 gttgccgggc gtaccaaaga tcgaccacca tatcgatgcg taaacctaag gtgtccggca
     3601 gctcgttacg acgggagata tccaccccca ggcttgccat cacatccagt tcggctcgat
     3661 cccataaagg ttctgagtcg ataagtaatc catccatatc aaaaattgca gcaagaatct
     3721 gacgcggggt tgacattaca acctctcctt tagagcatta tgtgtaaggt attaagccgt
     3781 gcttatgatt aagcgcctat catatcgcct ttaaagaatg ggcgaaaaca gatatctaca
     3841 ggtaaactaa gtgaaacgac gcgtcttaat caaggagaac tcatgacgta tcaacaagct
     3901 ggacgcattg ctgttttgaa aaggattttg ggttgggtga tttttatacc tgcattgatc
     3961 tctacattga tttctttgct gaaatttatg aatacccggc aggaaaacca ggaaggcatt
     4021 aatgcggtca tgctcgactt tactcatgtc atgatcgata tgatgcaggc gaatacgcct
     4081 tttttgaatc tgttctggta taactccccg acgcccaatt ttaatggtgg cgtgaacgtt
     4141 atgttctggg tgattttcat cctgattttt gtcggactgg cgttgcagga ttccggtgcc
     4201 cggatgagtc gccaggcgcg cttcttgcgg gaaggtgttg aagatcaact cattctggaa
     4261 aaagccaaag gggaagaggg gctaacgcgt gaacaaattg agtcccgtat tgttgttccg
     4321 caccatacta ttttcctgca gtttttctca ctatatatcc tgccggttat ttgtattgct
     4381 gcaggttatg tgttcttttc tctgcttggg tttatttaat caaaaggccg cacggagcgg
     4441 ccattatcct caacccatat catgctgcta ataatctatc caatgactgc tgagcaataa
     4501 ccaaatgctg accgccaaat aatcttgcac gatttaacag cgtgtagagt tggtaaaccg
     4561 gttgacgttc aaggaaatcg gcaggtagcg gggatactga ctgatagccg tcatagattt
     4621 gtggcggctg ttcagtatgc agcggtaaca tcgccaggtc gcactctcgg tcaccccagt
     4681 agcaggcagg gtcgaaaatg tacgggccat ccggacccag cgcacagttg ccggaccata
     4741 aatcgccgtg caacagagaa ggctgcggtt gatgcgaggc cagacgctgc tgaatatgct
     4801 cgacgatggc gtcgatattg ccgaaagcga tccctttctc tgctgccagt tccaactgcc
     4861 agccaatccg ttgttcagca aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg
     4921 gagttgtgga gagcgagtta tcaaaatcga ggccaaattg tggttggtca ctccattgat
     4981 gtaaacgcgc aatttgctga ccaagaataa atgcgctatg cgcatccagc ggacgaggtg
     5041 ggagataatc catcaccaga aaactgtagt cacggtcagc gcctactgcc caaaccttag
     5101 gcacggtgac ggttttacta cgcgacagta actccagttg gtcggcttcg gcggtaaaac
     5161 cgggaagcag ttccctttca tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat
     5221 gccatgcggc atggacttct ccgccaggca gttcattacg cagttcgatt tcgccttcac
     5281 ctaactgctc gctcaaaaga cgactgattg cctgccacat gatgtctctc ccatgttgtc
     5341 tgccagatca taaggttagc gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac
     5401 agactgatta ttgcccaaaa accatttatt ttctgttgct attccattcc tcccaggtcg
     5461 tgatggtgat ttcaggatct ttgccggttg cactttgggt aagccccgaa accagttctc
     5521 taatttcttc ttcactttgg gtactgataa gtccaaaagt gttagtcccc agctcatgga
     5581 tatttccctc atcatccgtc atggtgagca aaaaaccgtc gcgagtcagg taattattca
     5641 actcgttaat ttcagtcaat gtatcctcat gcaacatgac ggttatgaca taacggacaa
     5701 gatcgccact ggccatagtt cacccctttg ttatcacatg acttttgagc atagtcggag
     5761 aaacgcgttg ccgacaggtt ggtggtgatt cccccaatgc tgggggaatg tttttgttag
     5821 cgggaaaggt aagcgtaaat tttttgcgta tcgtcatggg agcacagacg tgttccctga
     5881 ttgagtgtgg ctgcactccc cgcagctacg ccaaaacgga ccatctcttc aagagaggca
     5941 ttttctgcca gtttcagtgt catcgcgccg accatgctgt caccagcgcc aacggtactc
     6001 tggcttttca ccggtggtgg caccacctga atgcagtttt cactatcaac acccagcgct
     6061 ccttgtggac caagggaaac gacaacccgt ttggccttgc cgctattaac gatttcctgc
     6121 gcggctttgc ggacatcgtc tggctgggtg agttcgcgat tcaccagcgc actgagttct
     6181 ttttggttag gcttaaccaa ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg
     6241 ccagaactgt cgatgatgca gcggatccct tggttttgcg cagcggaaat cagttgggtt
     6301 aatttttcca gcttcacacc tggcggcagg cttccgctta tgaccaggat ggccccggat
     6361 tcaatttcca gaacttgctc ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct
     6421 ggcataacaa aacgatactg ctcaccgctt gcttccacat gtacgtgtaa attctgccgg
     6481 gtccagtctt tggcttctac agtagcgacg gggacatttt catccgccaa cagagaaacc
     6541 aggtgttcgc cggtcgcgcc acccgccggg aagatcgctg tggcactgcc tccaagatgg
     6601 gcaatggcgc gggcgacgtt gatgccgccg cccccgggtt cgaacaccgg tgcggtacag
     6661 cgcagttttc cttcgggata aatttgcggg gtaattgttg cgctatcgag agagggcgca
     6721 agtgtcaacg tatagatacg taccatcatt tcctcctata ggctgatttc agtctggcac
     6781 cgaatcagcc gaaagtgaag aaattaacaa tatgatttat tagagcttta tttaaaattt
     6841 tgcagataaa tatatataaa taaaaatctg ttttcgccac atgcttattc aaaaaatgaa
     6901 ataagaatac attgctaagt tattcgcgcc tttttataat tcgttacctt tccagggacg
     6961 ctaatctttg atccttaaga aagtttccgg gaccgcgatg gtctcttttt ttgttgtacg
     7021 gactcttgat aaatgaagct tttgaagaca gttcctgcca tcgttatgtt ggcggggggc
     7081 atgtttgcgt cactgaatgc agctgccgat gattccgttt ttactgtcat ggacgatcct
     7141 gccagcgcta aaaaaccttt cgaaggtaat ctgaacgcag gatatcttgc acaatcaggc
     7201 aatacgaaaa gctcctcact tacggctgac accaccatga cctggtatgg acaaaccact
     7261 gcctggtcgc tgtgggggaa cgccagtaat acttcttcta atgatgagcg ttcttcagaa
     7321 aaatatgcgg cgggcgggcg tagccgtttt aatttaactg attatgacta tttatttggt
     7381 caggcaagct ggctgacaga ccgttataac ggctatcgag agcgcgatgt gttgaccgcg
     7441 ggttatggtc gtcagttttt gaatggcccg gtacacagtt tccgctttga attcggtcca
     7501 ggtgtgcgtt acgacaaata taccgacaat gccagtgaaa ctcagccgct gggctatgcc
     7561 tccggtgctt atgcatggca gttgactgac aacgctaaat ttacgcaggg tgtttccgtc
     7621 tttggcgcgg aagacacaac gctgaactcc gaaagcgcat taaatgttgc tattaatgaa
     7681 cattttggac tgaaagtggc ttacaacgtc acatggaact ctgaacctcc agagtctgca
     7741 ccggaacata ccgatcgtcg gacaacctta tcgctgggct acagcatgta aaattatctg
     7801 ccggaccgtg ttatgcggtc tggctgtctt cgtttgtcac tgctcagccc caaatcattg
     7861 ctgcccaacg tagcaacaac atagcgccac agattgccag tagcaccacc accatcttcc
     7921 agtgtttttt atcaaagcgt ttagtcggca taccagtgtc ctgcatatct cttgtgcgtg
     7981 ataacggagt gtaacaaaag ggacaggcat caagggattt tttgttatta cagccgtcgg
     8041 gtggcgttgt acacctgatc actgtttctg cgttttatga aaaaaataaa attatgtatc
     8101 gatatacaaa tatcataaat agctggttat aaatacgttt ttagttcatc agcactaaaa
     8161 ctcaggcttt ggctgattgc attaatagca gcataatatg tttctacagt tttataatga
     8221 ttatgatgaa tggctatatg aacagctgac atgttttcat gatttttagc ggccaacagt
     8281 gtgaataatt gacgttgact aaaagaatat ttttttgcaa aagtactcag tgttgatata
     8341 taagataaca cgacgtcttt attacccttg ctcatggcga tgtataaagc aggggttcca
     8401 tgtgctgtcg cgccttttaa taagtccatg atgttgattt tgctgagttt atatttaacg
     8461 gcgatcccat aaactttaga gaggaaccgt gtgacacaca aagggtgatt attttccatt
     8521 gcagcaaata accctggttc accatcacgt gttttcatgg ctaaaatatc catcaccatt
     8581 tttttgttta aaagttcttg tgctattgga gtggttaatg cattcagaaa gatatcgatt
     8641 atttcatcga atccatacaa catggagata tagagtccag gtgtttcgtt agcactttta
     8701 gtttgcagta atttaaccag attatcttca tgaatgatat gattgtatac cagtgactgg
     8761 atttcttgca taaatatttt tacgttgtca gtttgtccgt tcatcatagc cagaaataaa
     8821 gcaggtgtac caacgctatt ctgcgcagtt attatttcaa tgatttcttt ttcagggagt
     8881 atgggcaact gtttgaatag tgtaataagc tattgagtct ggcctgtggc cattattaac
     8941 aacattgccg atggatgtat aggggcaaat ggcattcgtg tcgtgatacc cgctaaaata
     9001 gcaactggaa ctccctcagg taatagagga agaatagcaa ttgcacagtt gcgaacatac
     9061 ctatcagccc aggtctgagc atcgggagct gaactgatga acttatcaag cgagagaaag
     9121 cgccgtgctg tcctgtgggt gcctctgacg gcaacgttgg tatcgttcgg gtcgtaaaca
     9181 gacacaacat aatttaattg tccatacact gactgcttta ttcgtagcct tactgacaaa
     9241 gtatgattgt ctatatataa aatcgcggac atatgactga cattgtttgc catcattcga
     9301 caaaagaaaa tatctaaagc gaagccaaaa tccttactcg cgataacgcg actccctgca
     9361 ggaggatggc gaagtaatgc tctgaactcc gcatcccgga ctaaggggat atgctgctgt
     9421 atggattcaa cggaggagta atgtttaatc aggttgactt tgccttttcc atttctcaat
     9481 gaatcaaggg cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc
     9541 aaaaaacatg cactaccatt gagatttact gttggttgta tagttttttc tctatggatt
     9601 tttttagaca gtttcataca atggttacga tcttcggcag ttaactgttc ctgctcaata
     9661 taatccagta tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga
     9721 atgatttctt ttttatagtg taatgtaata agtatatttt ctgcatggat gggtgaagtg
     9781 gcaaagttaa tgataaaaag ggcagtggag taacaatttt gtgatatttt tcgacagttt
     9841 gcggtaaatt cctgagtaat acagtatgac agcatcgaac ttaagtcaga ggtatcaagt
     9901 agggtgatga gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg
     9961 ctatcgatgt gcaatctatg aggagactta taatgagttc tgataatgat atcgttttga
    10021 ctcataatct attcacctta tatatgtctg gatgttttga ggatgatgat taattattat
    10081 cgccagaacg aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg
    10141 atatgaccac ttcgtcatta atctcatgat cgtttaaata ctaccgccaa atcacgcaac
    10201 aaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac ctttcagacg
    10261 ctccgtgatg ttatcagttg ttctttaagc gttttgctgg tgtactcact acaaacgaat
    10321 tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag tgttaacatc
    10381 gctcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg attgcgaacc
    10441 aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca aatttttctt
    10501 tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg cctgttataa
    10561 ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg gatgttgcgc
    10621 tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg
    10681 ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc gatcattacc gccaaagacg
    10741 aagaaggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcac gcgattaaac
    10801 aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt ttttattacg
    10861 acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag aagcggatgc
    10921 atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg cacgaagcgc
    10981 gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac gaaaacatcg
    11041 cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc
    11101 cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg gcaggggctt
    11161 actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag
    11221 acaaaaaagc gcttaacgct tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc
    11281 gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg ggtatggtat
    11341 tctggcacaa cgacggctgg accatcttcc gtgaactaga agtgtttgtt cgttctaaac
    11401 tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt gtcctgtggg
    11461 aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacgtct tctgagaacc
    11521 gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc aaccaggggc
    11581 tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc caccgtaatg
    11641 agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag gatgacgcgc
    11701 atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc cgtttagtct
    11761 atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc actcgtcctg
    11821 aaaaacgtat tggcagtgat gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc
    11881 tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc tacggtccga
    11941 aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt acagtacagc
    12001 tggacttctc tttgccttct cgtctgagcg cttcttatgt aggcgaagac aatgaacgta
    12061 aagtaccggt aatgattcac cgcgcaattc tgggatcgat ggaacgtttc atcggtatcc
    12121 tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccagttcag gttgttatca
    12181 tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg
    12241 cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa atccgcgagc
    12301 acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg gaatcaggca
    12361 aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta aatgaagtga
    12421 tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag gaataaggta
    12481 ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg
    12541 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag
    12601 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg
    12661 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt
    12721 ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg
    12781 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg
    12841 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg
    12901 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct
    12961 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagt
    13021 aaggccttca agtagcactg tctgtggagc cttcgggctt cacaggcttt gttcgccttt
    13081 gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt aagaccgtac
    13141 gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta
    13201 acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag
    13261 ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac gcataagccg
    13321 ttaacgtttt taacttttta attagaatat agatacagga gagcacatat ggctcgcgta
    13381 aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc taaaggctac
    13441 tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa agctggtcag
    13501 tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg gattgcgcgt
    13561 atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa tggcctgaaa
    13621 aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt cgacaaagta
    13681 gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag ttgaaagagg
    13741 gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta tcgccgtagc
    13801 cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta
    13861 ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg tgagaagaga
    13921 aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc gcgttttgaa
    13981 aaataaaaag cctgtctcat caggtgtaat tgtcgcagcc agtctggaca cggccagcgc
    14041 ggagaaaccg gagcgtattt aagtacgtga gaatttcgag cactgcccgg gaccaaaatg
    14101 gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt caccactgac
    14161 acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa ggcggccatt
    14221 agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa
    14281 gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga gcgtccggca
    14341 gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa
    14401 gcggaactgg aaagcgctgc actgaatgcg cgtctggcgg cggaaacgat tgatgtctcc
    14461 ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac catcgaccgt
    14521 atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga aatcgaagac
    14581 gattatcata acttcgatgc tctgaacatt cctggtcacc acccggcgcg cgctgaccac
    14641 gacactttct ggtttgacac tacccgcctg ctgcgtaccc agacctctgg cgtacagatc
    14701 cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg cgtttatcgt
    14761 aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct gattgttgat
    14821 accaacatca gctttaccaa cctgaaaggc actctgcacg atttcctgcg taacttcttt
    14881 gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgttcaccga gccttctgca
    14941 gaagttgacg tcatgggtaa aaacggtaaa tggctggaag tgctgggttg cgggatggtg
    15001 catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg tttcgccttc
    15061 gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct gcgttcattc
    15121 ttcgaaaacg atctacgttt cctcaaacag tttaaataag gcaggaatag attatgaaat
    15181 tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat gcgctggcga
    15241 atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc ggtagcttcc
    15301 acggcgtggt cgttggtgaa gtggttgagt gtgcgcagca tccgaacgct gacaaactgc
    15361 gtgtgacaaa agtgaatgtt ggcggcgatc gcctgctgga catcgtctgc ggtgcgccaa
    15421 actgccgtca gggcctgcgt gtggcggtag ctaccattgg tgctgttctg ccgggtgatt
    15481 tcaaaattaa agcggcgaaa ctgcgtggtg aaccgtctga agggatgctg tgctcctttt
    15541 ctgagctggg aatttctgac gatcatagcg gcattatcga gctgcctgcg gatgcgccga
    15601 ttggcaccga catccgtgaa tacctgaaac ttgatgacaa caccatcgaa atcagcgtga
    15661 cgccaaaccg tgccgactgt ttaggtatca ttggtgttgc gcgtgacgtt gccgtgctga
    15721 atcagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc gacgacacgc
    15781 tgccgattac agtcgaagcg ccggaagcct gcccgcgtta tcttggccgt gtggtaaaag
    15841 gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt cgttgcggga
    15901 tccgttctat cgatgcagtt gttgacgtca ccaactatgt actgctcgaa ctgggccagc
    15961 cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg atggcgaaag
    16021 agggcgaaac gttggtactg cttgatggca ctgaagcgaa gctgaatgct gacactctgg
    16081 tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc gaacactctg
    16141 gcgtgaatga cgaaacgcaa aacgtcttgc tggaatgcgc tttcttcagc ccgctgtcta
    16201 tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt tatgagcgtg
    16261 gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg ctgatcgaca
    16321 tctgcggtgg tgaggctggt ccggtaattg atatcaccaa cgaagcaacg ctgccgaagc
    16381 gtgcaaccat cactttacgt cgtagcaaac tggatcgcct gatcggccat catattgcgg
    16441 atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa ggcaaagacg
    16501 agtggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa gatctggtcg
    16561 aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta caggcaagcc
    16621 tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa acgctgctca
    16681 acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa gtgcagcaga
    16741 tgatccatcc aggcgttgaa gccttactgc tgccaagccc gatctctgtt gaaatgtcag
    16801 caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac cagaaccgtc
    16861 agcagaaccg tgtgcgcatt ttcgaaagcg gtctgcgttt cgtaccagat actcaggcac
    16921 cgttgggcat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac cgttacgaag
    16981 agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc gatcttgaat
    17041 ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa gcgaatccgg
    17101 cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt ggttttgttg
    17161 gggttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact ctggtgttcg
    17221 aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag atttctcgct
    17281 tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc gcagcggata
    17341 ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac ttatttgacg
    17401 tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc ctgatcctgc
    17461 aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc aaatgtgtag
    17521 aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc gcttacaaaa
    17581 gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga tgccaaagaa
    17641 ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga acaggtgaaa
    17701 ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg acgtaacccg
    17761 aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag acccgggcag
    17821 aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga tctaactaaa
    17881 aaggccgctc tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa aatcaacgcc
    17941 atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac agcgacaaaa
    18001 tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct taagcctttg
    18061 cgccggtgaa caatggatct cgccaggtga ctggtttact cctcgtggcg aactgttcgt
    18121 ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg cgctggctat
    18181 atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg gactacttgg
    18241 cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc aagggcaact
    18301 ccccaactgg gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca ctttaatact
    18361 cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg gcgttgcatt
    18421 agggattatc tgtagcgcac taatgacgtg ggctatctac ttttccacct ctgttgattt
    18481 acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc ggcaaagctg
    18541 gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca ggccgatgaa
    18601 tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt ggttctggcg
    18661 caatgtgctg gtggccgcga ccggctggat ggttggcgtc agtgtggcgc tggcgggtgc
    18721 tatcggcttt attggtctgg tgatcccaca tattcttcgg ttgtgtggtt taaccgatca
    18781 tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc tggccgatat
    18841 tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca ccgcaacgtt
    18901 gggtgcgccg gtgtttatct ggttattgtt aaaagcaaga cgttagccgc aaaaagacgg
    18961 tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat gcaagattcc
    19021 attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga gaagtacgct
    19081 ggtaatgtgc tgttgattgt caatgtcgcc tcaaagtgtg gcttaacgcc gcaatatgag
    19141 cagttggaga atattcagaa agcctgggcc gatcgaggtt ttgtggtgct gggattcccg
    19201 tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac ttactgtacc
    19261 accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg cgaaggacgc
    19321 catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc ggaagagagc
    19381 ggattctatg cccgtatggt cagcaaaggt cgtgcaccgc tgtaccctga tgatatttta
    19441 tggaattttg agaaattttt agttggcagg gacgggttgg tcatccagcg tttttccccg
    19501 gatatgacgc ctgaagatcc cattgtgatg gaaagcatta aactggcgtt ggcaaaataa
    19561 tgtctattgt gatgcagtta caggatgttg cggaaactac ccgcctgggg ccactttctg
    19621 gcgaggttcg ggctggggag atcctgcacc tggtggggcc gaatggcgcg ggtaagagta
    19681 ccttgcttgc acgaatggcc ggaatgacca gcggtaaggg aagcattcag ttcgcggggc
    19741 aaccactgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat ctttcacaac
    19801 agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat cagcacgata
    19861 aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat gacaaactcg
    19921 gtcgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt gctgcggtgg
    19981 tgttgcaaat cacaccacaa gccaatcccg caggccaatt gctgcttctt gatgagccga
    20041 tgaacagtct tgatgttgcg caacaaagtg cgttagacaa aattctgagc gcgctgtgtc
    20101 agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacgttg cgtcatgcgc
    20161 atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga gaagaggtgc
    20221 tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg gatatcgaag
    20281 gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat cgggtaatta
    20341 cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc gtttctgcct
    20401 tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac cgccgccaaa
    20461 tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc tacaaagctg
    20521 gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact gttcgggatt
    20581 tgtggttgtg acgatgcgcg atcgtttcga tttgcagctg ccccgtgaaa ccaaagaaca
    20641 agcctctatc ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc tggtcttttt
    20701 caaaacgggt tccggacaaa atggtttgca tgtaggtatt tacgatacca acaaccaatt
    20761 tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg tctattggca
    20821 gaaaaatttc tggcaggcga gacgaatcta gtatg
//
LOCUS       DAZGXZ010000090        20638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000090 DAZGXZ010000000
VERSION     DAZGXZ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.090"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..64
                     /locus_tag="ACHKJF_003430"
     CDS             <1..64
                     /locus_tag="ACHKJF_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0950732.1"
                     /translation="LEKLTSADEILAYRVGWAQE"
     gene            602..2227
                     /locus_tag="ACHKJF_003431"
     CDS             602..2227
                     /locus_tag="ACHKJF_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950733.1"
                     /translation="MPYIDITTMRGMMPGVIASMLPDHSAVLAENCHFRYGVITPEHQ
                     MSEAEKTFAIKPKTIFHYRDDFWFAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLSPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPGKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDVNGNTALATEKIISPEQWQSQFNPASIVAYSWRGE
                     YIACYTKPDGKQDVFVFSPVNMDIRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGSS
                     LPSTIRWHSKIFSLPERTSFSCIRVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVR
                     LPAATGQNWQVMVSGFGQVERITLSTSMSEMPV"
     gene            2224..3492
                     /locus_tag="ACHKJF_003432"
     CDS             2224..3492
                     /locus_tag="ACHKJF_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0950734.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            3507..3785
                     /locus_tag="ACHKJF_003433"
     CDS             3507..3785
                     /locus_tag="ACHKJF_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950735.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            3791..4408
                     /locus_tag="ACHKJF_003434"
     CDS             3791..4408
                     /locus_tag="ACHKJF_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950736.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLVINTSVYD"
     gene            4499..5233
                     /locus_tag="ACHKJF_003435"
     CDS             4499..5233
                     /locus_tag="ACHKJF_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0950737.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGVTAGIGLKF"
     gene            5466..5606
                     /locus_tag="ACHKJF_003436"
     CDS             5466..5606
                     /locus_tag="ACHKJF_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0950738.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            5958..7184
                     /locus_tag="ACHKJF_003437"
     CDS             5958..7184
                     /locus_tag="ACHKJF_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0950739.1"
                     /translation="MKMTGKLLLAFVPALSLSVFSADAYAVDTSANSSGISGSPYNGI
                     TVDVLKSKQYADINSFWNDLGFPGKGYQDGSIVKVENGKITGPASVDIQNTAGKYLKY
                     SLYDAGKSGLTNTELLAPRSEIASYYDYANKVAYFVNTKTNQILFSADDKGLVTTVGT
                     DGKTYSRHIPEISEYIYEIKIKSVIPGSTINTVLFAEDVTDIHARLSALGSGETQVGG
                     GATAGGENATAVGYNATANHTNSVAVGANSQTTRPDEVNVGNRVIGGLRDGVQAADAA
                     TYGQLERYRIAEENARIQGDAQTLKSANTYTDNRVSTLERSTNQQFRQLRDEVEKNRK
                     RADAGIAGAMAMTAIPVVEGKTYSFGMAASNYRDEQAIAAGMHFRTTENSAVRLNASW
                     DTQNGSGVAAGMSVGW"
     gene            7320..7673
                     /locus_tag="ACHKJF_003438"
     CDS             7320..7673
                     /locus_tag="ACHKJF_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950740.1"
                     /translation="MQRVVRNSRNPEFMNEVKDACLKKQAFCFEAPDGFLVLRSVLSA
                     DGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHTARRGFIRLGKRLGFER
                     MPDDEDGFMVFRIAV"
     gene            7767..8423
                     /locus_tag="ACHKJF_003439"
     CDS             7767..8423
                     /locus_tag="ACHKJF_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950741.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDDLNSEQQYENIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAAGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            8426..8872
                     /locus_tag="ACHKJF_003440"
     CDS             8426..8872
                     /locus_tag="ACHKJF_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950742.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNPQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            8882..9154
                     /locus_tag="ACHKJF_003441"
     CDS             8882..9154
                     /locus_tag="ACHKJF_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP0950743.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRRTEIEASNKQLAAQQKAQ
                     KKQNIGTGIGTGATIGATVGSAVPVVGTAVGAVAGAVIGGIAGALF"
     gene            9165..10430
                     /locus_tag="ACHKJF_003442"
     CDS             9165..10430
                     /locus_tag="ACHKJF_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950744.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQKNNERDFE
                     FAQSQFEHNKDVDQRNFDYRAKVDDRNYTLQEREFNANQNYRNASLGMEQQRLQLQKY
                     NQRRLEYNDMLARDQPVMAALGKAIDAGDHDAASHLFGKLSDANPLKMMSTVGYAAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFAPELQQRIGMPDSTGEKTIKEAR
                     IGSIVPAQQEGYVLIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            10500..18881
                     /locus_tag="ACHKJF_003443"
     CDS             10500..18881
                     /locus_tag="ACHKJF_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP0950745.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVVGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEETAMRDAETVQQDDAA
                     PQPESVDPVAQQRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQAPAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDIARNVRMPQPESLPRTVRDSLPEL
                     AQQAEVRRQAGENRDILQPETIAPESETTVSTDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYATQPLSKLFPKPDYAKLANEGADADTLAMIALYRSDI
                     PAKTKHNTAGWGESIKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDRASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDSHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGNVSPEQFSDAFGFR
                     GVQFGNYVEGPRRQADLNRAYDSLHDLAEVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYEPGEAAINLTKGNGPGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEARRVKR
                     VFKDGRYVDAEYPVRQEVYDAFKGVVQAIKNSDMPRRSALLDEVRSKPYWSTNVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGVTRTPEGNLISDVNRSKEAKGSPVPQVEAVARGVMSGIKDS
                     DLKVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGVGASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKAVRSRLEINKVASVYGTENGKKLKSMEMTGLTLYRREKL
                     SRDNLLHRGLQLPKGEHSYRGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKVDDSNNAYMAEELFHGKAESDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQNAYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESRQAMGRASRAQSPSTQAIQDLSESLIRHRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRTIAERKDQETGETIREVVERPVAMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGKGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGDGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPIGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKYWVDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEVAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSTLSLAQQSAMVKS
                     VERDMKIAVDRFNREYNKKVGVE"
     gene            19241..>20638
                     /locus_tag="ACHKJF_003444"
     CDS             19241..>20638
                     /locus_tag="ACHKJF_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950746.1"
                     /translation="MSKKFTKTLLSSSLSGLLLGISSGVLAQELPPIKDSGLPFYSVL
                     KEADTSSVIYGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAASLRTPDKENGKHQVDEEEVRRIFNKVNNLSNTIINPEVLKQSG
                     FIASGPRPEGDRLVQASAAGELALAVGEGAAAEEKNSTAVGSWASAHGRQSTALGSGG
                     QAYADGSTALGSYSHVDDSSVYGVALGDSSVVGENAPEGTALGSRAEVSHRNSVALGA
                     RAHTTRDNEVYIGYVEEAGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQD
                     TNTRLVVEAKKSREYTDSRTTVGVNADGTLTSADGATKTIAVNDGLVALSGRTDRIDA
                     AVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAALAGLF
                     QPYSVGKFNATAALGG"
BASE COUNT         5395 a         4479 c         5937 g         4827 t
ORIGIN      
        1 gctggaaaag ctgaccagtg cggatgagat actggcatac agggttggct gggcacagga
       61 ataacagaca aaaagatggg ggaccatcac ctcccccatg aactgagtca attatacact
      121 tgtgaagtat ttactatgcg aataaatact gtcacggaaa gtgcaggcct ggatgtgatg
      181 gaaatcatgt ttccggcgca tcctgttgat atggataatt tttcagattt ccggaagtga
      241 gagtttttgg tgtttccata ccagccacag ttgatgtgtg ttgtggcgct tggcagtgcg
      301 attaaaggag cagagggaat aaaaaacggg ggcatgacca acccccggta caaggaagga
      361 gtaactacaa tggaaaaatt aatgaagacc tttagtgcta cgttatatgg cgaataaaga
      421 ctgccacagg gaagtttgca gaaacagtga tgccgatcgt gtttctgacg ctcttctctg
      481 ataagagtaa tttttcagat ttctggaaat ctgtattctg cacaggcgca attgtatgcc
      541 gcctttaaaa cttcaattca gtgactcacc tgccattcaa attttcggat accagacaac
      601 catgccttat atcgatataa caactatgcg cgggatgatg ccaggcgtta ttgcatctat
      661 gctgccagat cattctgctg tactggcaga aaactgtcat tttcgctatg gagtgatcac
      721 gcctgaacac cagatgtcag aggctgagaa aacattcgcg attaagccga aaaccatttt
      781 tcattaccgt gacgatttct ggtttgcatg gacggatgtg gtggatgtga tccgcagtcc
      841 ggtcgctcag gactcccacg ggcgtattta ctacactgac gggcgttttc ctaaagtgac
      901 ggatgcgacc attgccacaa aaggggacgg gaatcacccg acatcatcgt atcgtctggg
      961 gatccccgcg ccgacgacag cacctgtctg tactgttcag cagggcggtg atgtttctga
     1021 cgataacccg aatgatgacg aaacccggtt ttatacggaa acctttgtct cagattatgg
     1081 tgaagaaggt ccgccaggtc cggcgtctct ggaggtaaca ctccgtactc cggggactgc
     1141 ggtacagctg acgctgtctc cggtgccatt gcagaatgcc agtattaaac gccgccggat
     1201 ttatcgctct gcatcaggtg gaggagaagc ggatttttta cttgtggctg aactggatgc
     1261 atccgtgctc agttacacgg acaaaatacc ggggaaaaac cttggacctt ctctggcgac
     1321 atgggattac ctgccgccgc cagagaatat gacaggcctt tgcctgatgg ctaatggtat
     1381 tgccgccggg tttgccggta atgaagtgat gttttcggaa gcgtatctgc cgtatgcatg
     1441 gccggaagtg aatcgtcaca cgacggcaga agatattgtg gctatctgtc cgctgggaac
     1501 gtcactggtg gtggcgacaa agggggagcc ctatctgttc agtggggtat cgccttccac
     1561 aatttctggc tccagaattc cttccatgca ggcatgcctg agccgaagaa gtatggtggc
     1621 gatggaggga ttcgtactct atgctgggac aaacggtctg gtatctgttg atgtaaacgg
     1681 taatacagca ctggcaacgg aaaagattat ttcacctgaa cagtggcaga gtcagtttaa
     1741 cccggcgtcc attgtggctt attcctggcg tggtgagtac attgcctgtt acacgaaacc
     1801 ggatggtaag caggatgtgt ttgtattcag tccggtgaac atggatatcc gttatctcag
     1861 tacaccgttt gactgcgcat gggttgatct cgcgaaagat atgatgcgcg tggtgacagg
     1921 agacaaaatg tcagtgcttg ccgggagctc tctgccctcc acgataaggt ggcattcaaa
     1981 aattttttca ttacctgaaa gaacctcttt ttcctgtatc agggtgaaat ctccggcgcc
     2041 tgagcgggtg gggatcacca ttatggctga tgatgttcct gtgattcatt ttgcgccggg
     2101 tacgtttaag ggaagtgtgg tgagacttcc ggcagcaacc gggcaaaact ggcaggtgat
     2161 ggtatccgga ttcgggcagg tggaacgaat aaccctgagt acatcgatgt cggagatgcc
     2221 ggtatgacca gaaaaccgtg gcgtgcgggg aaggatttat ccacagttgt ggagaacatg
     2281 gaaattggca ccgggcagcg tggtgacgga cgccacgcat ttgtgacccg tgaggaactg
     2341 gttggtctta aactcgcccg gcgtcgaaca tcgggtggtg cctcatatgc actgaatccg
     2401 ggtattgaga ttgacagtac tttaatgact gttgattttc ccacaaaacc gctgaatttt
     2461 aaggcgacag gaggatttgg ctcggttctt cttgaatggg atatgcctaa ttatcgcgga
     2521 cattcactga ctgaaatctg gcggggtacg gaggatgacc ttgctgatgc agtgctggtt
     2581 gccacgacgc cggggcaggt ttacggcgat ccggttgacc ctggctggtc gggattttac
     2641 tggatacgtt ttgttaacgc ggcaggagtg aaaggtccat ggaatgctga aaaaggcact
     2701 caggcacaaa cacagatcgg cgtgaaggcc atcattgacc agatccgcga tgaggctgca
     2761 aagtcgccgg ttgtgtccga gctgcgtaaa gaaataaaaa acgcgcaggg gcaggctgta
     2821 aaggatgctg caattaagac aaccgaagtt gtggggactc tcagggaaga aacgacaaga
     2881 acgattggtg gtattgaaac ccgcattagc acactggatt cgtcaaccag tgaatcgctt
     2941 aatgaggtcg acaagcgcat cactaaactg gataaagaag gcggtgaggc gtttctggca
     3001 atgtggtcaa aaaaagcggg agttgatggt atcactgcgg ggatcgggat tgtcgccgga
     3061 aaagacagtg aaggcaggcc tgtaagtcag gttgcaattt ctgcgtcgca gttgtttgtc
     3121 tttgacccga acaacccgga taacaccgcc tatccgtttg cggtatcagg tggcaaggtt
     3181 gtgatcccga aagcgatgat ttatgacgcg gtgattgaaa cactggtgtc gcggaaggtt
     3241 gtggcggatg aggtaaaagc cggggtaagt atcacttcgc cagttatccg gagtgccgtt
     3301 attcagaacg gaaactttca ggttgattct cagggtaacc tgaatattgg aggccttttc
     3361 agtgttacgt cacaagggca actgacaatt cgttactcta atcagaatgt aggactggtg
     3421 atccgcaatg ataaaattga ggtttatgat cagaatggac gactggctgt tcgcataggc
     3481 agattacgct gatcaggagg tgagtattgg aatacggttt tgccatttat aacagaaata
     3541 acgttaatgt tacgggcgtg ctgactccgg tatttttcct ggacagattt acagcggagt
     3601 ctggctcaaa gacgtacacg aataaacccg acgggaaatc attgcaggct gtatgttgtt
     3661 tatttccctg gaataatgta tttgcggatc ggaaagtacc gaagataacc attaatggca
     3721 atacggtgac gtggtcgaat cttgagcagg gtatgggatc ttatatttat acattctggg
     3781 gataagtgtc atgtatggtt tgagcattat gaagccggat ggcagcgtat ggataagtcc
     3841 aggttttacg ccgcagtgtc tgatcaacaa aggcaccata ccggcgactg aaaagtcttt
     3901 ttttaaaaca tcaatcccgt caggcaaaag ttgttttttc tttatcagaa cagagaagaa
     3961 ggccgatgtc atgtacacgc atgaacagat tgatggatat catgcactaa ggcttcatgt
     4021 aattgtcagg ggaacgaacc ctggtgttac gacggtttat gctttcgcga atatggttac
     4081 tccaccttct gagtatggta tcgccatgta taacccggac ggtgagatga tttatcatgg
     4141 cgaaatgatg ctgcttgacg cgaagttaat acctgttgat atcaaatttg aaaaggacct
     4201 tggatatcca tgcgcaatca tgcctgcact ggtcgggtat tataactgga aaagaactcc
     4261 ttatgatcga ccgatttata ccacatccac tggtgctaca ggaaataaaa tatattcctg
     4321 tgagcattat tccggtggtg caacatggga tattcgaaag ccgtatatag ataaggtcct
     4381 ggttattaat acatcagtat atgattagtt gaagcgagtc tttaatattc atttaaaatg
     4441 tctaaaaaga tgtattatta aaaagtttag cgtgttatct gaatacagga tatcttaaat
     4501 gaagagtata gcaacactgg ttgtgtgtgc aatctccggg attgcctgtg taaatttatc
     4561 tgcacatgca gcagaaggaa accatacaat ttctctgggg tatgcgcact ttcagtttcc
     4621 gggactgaag gattttgtaa aggatgcgac tgctcataac agggagactt tcagtcattt
     4681 cgtcaacaga aactactttt cttcattggg cgaatataca gatggtcggg tcagtggata
     4741 tgaaggcaag gataaaaatc cacagggcat taatatcagg tatcgctacg agataacgga
     4801 tgattttggc gttatcacct cttttacatg gacgcgttct ctcactaact cacagacatt
     4861 tattgatgtg cagtcagccg atcataccag gaagattaag aatccggcag cttctgccag
     4921 aacggatatc agggcgaatt actggagtct gttagcgggg ccttcatggc gggttaatca
     4981 gtacatgagt ttatatgcga tggcagggat gggcgttgct aaagttagcg ctgacctgaa
     5041 aattaaggac aatattaaca gtagtggcgg attttctgaa agcaacagca cgaaaaaaaa
     5101 ctcccttgcg tgggctgcag gtgcacagtt taacctgaat gagagtgtta cactggatgt
     5161 ggcttacgaa ggttccggct ctggcgactg gcgcacgagt ggcgttactg ctggcattgg
     5221 cctgaaattc tgacctgtat ccggtaaccg tttactaccc gctgtgatgg cgggtttttt
     5281 attgcccgta cagggcaaaa accgtaaatt atgcgtgggt gcctttcggc tgatggctgg
     5341 agggagaacc tgaaggcctg atgtggaaag gccccgagtc aactttaacg ttaacccgag
     5401 gccctgacca tcatacctta agcaagtaga aggttagcgc ctctctgtaa aaggagtcaa
     5461 gcgctatgtc gcaaaaatcg cttatcaccg tcacaatttg tatgacggtt atcttcacca
     5521 tctggatgtt acacggctcg ttgtgtgagt tccggctgaa tttgtgggga gcggagtttg
     5581 cggcgttctt acagtgtaag cagtaggaaa accgcggcgg ggacgaaagt ccccgtcaac
     5641 tggttgctga ggttcagtcg atatggcacc cgtttgtaga gtgtctttca tccaatcccg
     5701 gacaggccga gagaggggat atgtgtgaat gggaaataat aactattgga aatgttttgt
     5761 tttttctaag tttttttggc gctgtgtata tgaatttttg atgtgttccg tattttgata
     5821 tttttaaata ttaataaatc ttaacttgtt gttttttatt attttattta ttttgtagtt
     5881 gtgagtatta tgcttgacga catccgtgtt cccggatgtc gatgcattta tgacataatt
     5941 aacacgggat aaacatcatg aaaatgacag gtaagctatt gttagctttc gttccggcat
     6001 taagcctgtc ggtattttct gcagacgcat atgcagtaga tacatctgcg aactcatcag
     6061 gcattagtgg ttcgccttat aatgggataa ctgttgatgt tctgaaaagt aaacaatatg
     6121 cagatataaa ttcgttctgg aatgacctcg gatttccagg gaaaggttac caggatggca
     6181 gtatagtaaa ggttgagaat ggtaaaataa caggacctgc cagtgtggat atacaaaata
     6241 cggctggtaa atatttgaaa tattctcttt acgatgccgg taaatctggt ttgactaaca
     6301 cagagttatt agcaccacgt agtgaaatag catcttatta cgactatgca aacaaggtcg
     6361 cttattttgt caatacaaaa acaaatcaaa ttctgtttag tgcagatgat aaggggcttg
     6421 tcacgacagt aggtactgat gggaaaacat attcaagaca tattcctgaa attagtgaat
     6481 atatttatga gattaaaatt aagtctgtaa taccagggag caccattaat accgtacttt
     6541 ttgctgaaga tgtaacggat atccacgctc gtctgtctgc actgggttct ggagaaactc
     6601 aggttggtgg cggggcaaca gcaggcggag aaaacgccac tgctgtcggt tacaacgcca
     6661 ctgcaaacca tacgaacagt gttgctgtag gggctaactc tcagaccact cgccccgatg
     6721 aagttaatgt ggggaatcgt gtaattggtg ggcttcgtga tggtgttcag gctgcggatg
     6781 ccgcaaccta tggtcagtta gagcgctatc gtattgcaga agagaatgcc cgtattcagg
     6841 gtgatgcaca gacactgaag tctgctaata cctacacaga taaccgggtc agtaccctgg
     6901 agcgtagtac taatcagcaa ttccgtcagt tacgtgatga ggtggagaaa aaccgtaaac
     6961 gtgcagatgc aggtattgca ggagcaatgg cgatgactgc cattcctgtt gttgagggta
     7021 aaacgtactc ctttggtatg gctgcaagta actaccgtga tgagcaggct atcgcagcag
     7081 gtatgcactt ccgcaccact gaaaattctg ctgtacgttt gaatgcttca tgggataccc
     7141 agaacggttc aggtgttgct gcgggtatgt ctgtcggctg gtaatttatt ttttgcggag
     7201 aacgccagcc gtgttggtta tcggaagcgt tctccgtcat ccttctgtcg ctgaagacac
     7261 agcacagtca cattgttctt ttttgtggtg agagtgtgag tgaaaaaatt ctccggtata
     7321 tgcagcgtgt ggtgagaaat tcccgcaacc ctgaatttat gaatgaagtt aaagacgcct
     7381 gccttaaaaa gcaggcgttt tgttttgagg cacctgatgg ctttctggtg ctgcgttctg
     7441 tgctcagtgc tgatggtatc ccttatgttc tggtgttgct gggcgtgtgt acggggagta
     7501 acagcgttga gcgttatctg ccggaggtga agacattaac ccgtctggct ggcggacgct
     7561 gggctgaatt tcatacggca aggcggggat ttatccggct gggaaaacgt ctgggctttg
     7621 agcgaatgcc ggatgatgag gatggcttca tggtgttcag gatagcggtc tgactgctac
     7681 agttttcatc attgtgttta aaccaacatt gtaattcaca ttctgaccct gctccggcag
     7741 ggttttttgt tatccagggg gccattatgg gtggaagtaa aggcggtggt gatactaaag
     7801 taaaaccaac agcagcgcaa atagcgcagg aagaagttgc ctggaaaggg tggcaggatt
     7861 atcaaaaaat cctccgtccg gcagaagata acttcatgga aaaggtcgat gaccttaaca
     7921 gtgagcagca gtacgaaaat atcgctggca caacaaatct tggttatcag aaacagtttg
     7981 gtgaagcgcg aagggagctg gcgggtaatc ttgctcagtc cggcgttgac ccatccagtg
     8041 gtcgttttaa tgcggtaatg gatgcaaatc aaagcgatca ggtgaccggg cagattgaca
     8101 caaccacacg ggggcaggtg tcgcaggcag ataagtatgt tgccgggcta caggatgttg
     8161 ctgctctcgg ttctggtcag aaggcggatg cgttacagag ttttaactct ctggcagaca
     8221 gcagtctggc aaaagctaaa tcggatgcac aggcggcgtt tacgaaacag caggggcgag
     8281 cctctcttgt tggcgctggt ctgggtgcgg caggtgcata tgcgatgcat aaggctggcg
     8341 gtagcggagg aagtggcggt gctaaaacac ctggcaccgg cgctaatgcc attcagcatc
     8401 aggctcagaa ctggagactg tgattatgga gtacggtaaa tacgaaacac ttgcaagata
     8461 cggttatacc ggagcagccc gtcctcaggg ggactggcag acatccgcag cgctgacccg
     8521 ccagcaatac gacgactggc gcaccagata tttaccccgt gtagcaaggc tggctgacct
     8581 tggggagaac aacagtctga tgaatccaca gcttgcacgg gtgggaggcc ttgccacttc
     8641 cagtctccgt acagcgcaga tggcgcagga taaccagatg gcgagatacg gggtaagccg
     8701 cccggataat cccgacagta atacgctggg gttacgtaat gccctggcaa tagcaggtgc
     8761 gaaaaatggt atccgtgaag ccgaacagga tcgccagatg aatattctga cgggggcttc
     8821 tgcaccggca agacagaaac tgagtgttgg cggacaactg gtggcagcgt aagggggcaa
     8881 tatgggatac ggtttactgg atattgcaaa tcagtcgcgg cgtgaggcat tacagggaat
     8941 aagtgacgca gacagacgac gtacagagat tgaggcatca aataaacaac tggctgctca
     9001 acagaaagct cagaaaaaac agaatatcgg tacgggcatt ggtacggggg ccactatcgg
     9061 ggcgactgtt ggttcagcag taccggtagt ggggacagcc gtcggcgctg ttgctggtgc
     9121 agtaattggc ggcattgcag gcgctttgtt ttaaggagtg gtgaatgagc ggatttgcac
     9181 aggggttact tgccggattc agcactgttg accaggcaat gacccgtcgt aaggagcttg
     9241 gtttgcgaga agcacagctt gctcagcaac agaaaaataa cgagcgcgat tttgaatttg
     9301 cgcagtctca gtttgaacat aataaagacg ttgatcagcg gaactttgat tacagagcca
     9361 aagttgatga ccgcaattac acactgcagg aaagggagtt taacgccaac cagaattacc
     9421 ggaatgcgtc gctggggatg gaacagcaac gactccagtt gcagaaatac aaccagcgac
     9481 ggcttgagta taacgatatg ctggcgcgcg atcagcctgt gatggcggcg ctgggaaaag
     9541 cgattgatgc gggtgaccat gatgcggctt ctcatctgtt cggtaaatta tcggacgcca
     9601 atccgcttaa gatgatgtca acggtaggct atgctgcgaa agcgggtcag gccgtgaaca
     9661 acctgcagaa aatctttgat gataagccgg acagggcgat tgcttcgctg aataccccgg
     9721 aaaatctcga tgtgctttcc ggcgtgtttg ccccggaact acaacagcgt attggcatgc
     9781 ctgattcaac cggggaaaaa acgataaaag aggccaggat tggcagtatc gtaccggcgc
     9841 agcaggaagg gtacgtactt attggccttg atctcacata cagcgatggc tccaccgcgc
     9901 ataaacctgt aacagaatac ggcagtgcgc accctgatga tcaaaccgtg ctggcgatac
     9961 ccgttgataa ggctatcgct caggtcaggg atcgcagcaa atttgcagag atatcgaaaa
    10021 attatggtta ttttatgccg aagcagcagg gactttctct gaaagagctt cagaaggggg
    10081 ccagcaacgt agcggcggac gcgatcaaga atggcggtaa tgctcaggct gcggtggatg
    10141 aatattatgc tgcgactggt tcacaaccgc atcaacagaa aattcagcaa caaaaacttc
    10201 agcaacaggt tatcaactgg gcgggagatg atcctgataa gctgtcattt gccagaaatg
    10261 tagcggcccg tcagcctgaa atgctggaac ctcagaatca gaaattgctg gagaacgggt
    10321 atgcgaattt tctccgtatt caaaaggcca ggggggaaca ggccagagat gaaagtgctt
    10381 catctgcatc tcagtttatc cgtggactga aacagaatta cgcccagtaa ttcacgatat
    10441 tccattaata ccatttcctg atgcccggcc attgtgccgg gtttttttat ggagtctgta
    10501 tggcctattc agaggaacag cgtcctgagg cgcaactcgg caaccagaat cgtaacagcc
    10561 tgaacattca gcaacccggc gaaactgaca gctatgaagc atttttctct gatccgaatc
    10621 gctggaagga taacagtacg tcgttcagcc tgggcgatgt attgccaaca atgggtaaag
    10681 gtttcgccca gtccgtccgg ggaacagggg aaatggcccg tggactcggt gatgcgatga
    10741 ttcagagccc ggtaaaaaca ggggcgcgta ttttaaatga gttcagccgt atggggctgc
    10801 cgggtgtcgc aactgtgcag gatatttttg ccggtggcag caggggggct gatgaggtca
    10861 tcgataccct gcctgatggc aaaaacgcgg ttactgatac tgtcggtaaa ggtctgaagg
    10921 caaccggtaa ggctgtcagt gatggtgctg aagccactga tgaatggctg accggtaaga
    10981 tgtcgccggg tgcagttcgt gcgctgaata cgccgatgac cgaaggctat gatgattctg
    11041 cggtctgggt ggcgaagggt gtaaacctga ttggtgcgct tgtacctgat atggttgttg
    11101 gcggtgtggc taaaaaggtg ggtgatgtca cactgcgaaa aatgctgacc gccgggctgg
    11161 agaaaaaata catcgcggca gggatgcagc cggaaagagc cacggcactg gcagcagaag
    11221 ctgtcgataa aaaaatgccg gatttattcc aggcgggcct gatcacccat tccacagcca
    11281 gtgcacaggg gcagagtgca atggcggcag cagatgctgt tcttaatgcg gattactctg
    11341 agctggcgca gtcaccgaaa tttcagcaga cgtttttgtc cattgacgcc gacccgcagc
    11401 acgcacagct tactgatcgc cagaaaatgg atctggcaaa agagcgtgtt gccgatgagg
    11461 tgcgcgcgca gctggcaacc gatcctgaat tgctggctgt gaatgccatg gcggcaaaac
    11521 tgggtgacgc acaactgttt aatctggtga cacgaggcac agcgaagacc gttaaaagcg
    11581 gcattgtcag aaatgccacg gaacaggggg cgattaatgc ggcgcagggc ggctattcac
    11641 gctatcagga aaacacggca ttgcgtgaga ccgccggaat ggatgtgtca ccgtgggagg
    11701 gcgtggctga cgcaacgatc gaaggtgcag cctttggtgc tgcgatgggg gctccatccg
    11761 gtgcggttgc cggatatcgt ggcagacgtc aggccgcaga agaaaccgcc atgcgtgatg
    11821 ctgaaaccgt gcagcaggac gacgcagccc cgcaaccaga atctgttgat ccggtggcgc
    11881 agcagcgtga atccatgcag ggcatgaatc gcgagcagct tctggagcag tatgctgatg
    11941 cggatatggc aacagagggt gacgcatccg cagctcatcg ccgggaagct gccagccagt
    12001 tgttgaatga actggacgaa caggcgaagc gacaggctgt gatgaatgag ctgaaggcga
    12061 agccgcgttc tgaactgctt gaggaatacc gcagactcag ccagaaagag gagcgcaccg
    12121 agactgaaga acaacagttt caggcaatac gagaagtcat tcgcccacaa caggaagtga
    12181 cgccggaagc acagtcacag cctgaaaatg cggaggatgg taacgggagc atttacccga
    12241 cggtgcggtt ccgggacccg aatgaagtcc gcattgaaat taacgggaat ggtgcgtcca
    12301 gaccagcgga acgcattgaa aaggtgcgcc cggacaaccg ttatttcacg gatgagaaaa
    12361 gcgccatggg gagtgatgtt ttccgtaatg ccgccgccac cggcctgaaa ccgtccgtag
    12421 tgaagaaagg cgagaatcaa tatgccgttg aaatggataa tcctgcgttc tctgaagatg
    12481 tggcaacgga aaccattaac accctggctg acggagagcg tattgctgat gctgacccga
    12541 tggagcagcc cgcgttcatg cgtgacccac gattccgtgg tttcacgggg gatgatacgg
    12601 aggtacaggc ccgccttgcc cgtggcaacg tgccgacggc agaggagctt gtacgttcac
    12661 agatggctga aggtgatgcc ggtccgacag cacaggagtt aactgagcgt ccacgcctgc
    12721 ccgctcccgg cgatattcat cccggacagg gatatccgtt accgggagag gtggcgcgta
    12781 cgccggatga gaatcaggca ggacgtggtg gtcgttttac cacaaccggt gaggttaagg
    12841 gccagagttt ccagaaagga caagctccgg caccggaaaa cgccgctggt cgccaggggg
    12901 aaacactcga gggtgaaatg gttcgtcgtg gtctgccgtc accggatgcg cagaacgcga
    12961 cagcaccggt acgtgaaggg ctaccggctc ctgacattgc gcgtaatgtt cgtatgcctc
    13021 agcctgaatc acttccccgc actgtacggg actcactgcc tgagctcgca cagcaggcag
    13081 aagtacgtcg gcaggccgga gaaaatcgtg acatcctgca gcctgagaca atcgcacctg
    13141 aatctgaaac aactgtctct actgacaggg aagctaccgt gcgcggaggt gaagtcaggg
    13201 gcaaaaaaat tgaagacttt ggtgaagaaa ttaagggcgc ggcaaaacac cgttatgcac
    13261 agcttgctga aacactgggt aaaacgctgg aagacaggga ttatgccacg cagccgctga
    13321 gcaaactgtt cccgaaaccg gactacgcaa aactggcgaa cgaaggtgct gatgctgata
    13381 ccctggcaat gatagcgctg tatcgtagcg atattccggc gaagacgaaa cacaatacgg
    13441 caggctgggg ggagagcata aaaaaagtac gacacagtgt atcggaaatg ctgaacggaa
    13501 cggtcagcgc gaaacgcctc gcagaatgga tggaaggcag aatgccctcc cgttacgcgg
    13561 atacctggca actgttacgc actctgccac cctcacagat ggacagggct tctgcttatc
    13621 gggtggtatc gggtgtgtat caggcggcag gagggaagcg ttacgatccg ccacagaaac
    13681 tttattcact gcgcaataag gacaataagg ggagtaacct ctttttctcg gaaagcaggg
    13741 atgaattact ggcaaaggcg aaagtctggt ttgcagagca ggaggaaaaa tcacaggcga
    13801 aaggtgatga aaaaacagca ccgtcaccgg atgacaaaat ccgctttgac gtttaccgga
    13861 atacccgcag tggcgatatt tttatcgctt acggtaaaaa caaaatgcgg gtgagaggtg
    13921 gctttaagtc agccagtgat gcgcgtaagt acattgattc acatcgtgat gagcttgttc
    13981 gtcatgtgaa ggagatgcgg gagatttcgc gtgaggagca gcgcaacgct accaaccgcg
    14041 accgtaccgg accagaacgc cgcaagggga atgtttcacc ggagcagttc agtgatgcgt
    14101 ttggtttccg tggtgtgcag tttggtaatt atgtggaagg tccgcgtcgt caggctgatt
    14161 tgaaccgggc ttatgactcg ctgcatgacc ttgcggaagt actgaatgta ccgacaaaag
    14221 cgctttccct gaacggtcgt cttggcctgg catttggtgc ccgtggtaag ggtaaggcgg
    14281 cagcacacta tgagccgggt gaggcggcaa tcaacctgac aaaaggtaac ggaccgggtg
    14341 cgctggcgca cgaatggttc cattctctgg ataattattt tggtcgttat gacgtttcca
    14401 ctgacgggaa aattacgtca ggtggcgact atatgacgga agcccggcgt gttaagcgcg
    14461 tatttaaaga cggcaggtat gttgatgctg aatatccggt acgtcaggag gtttacgacg
    14521 cttttaaagg tgtggttcag gccattaaaa acagtgacat gccgcgtcgt tcagcacttc
    14581 tcgatgaggt gcgctcaaaa ccgtactggt caacgaatgt tgaaatggcg gcacgtgcct
    14641 ttgagcgtta tgttcaggat aaggcgcgta tggctggcgt ggagaatgat tatctggtca
    14701 atatccgtaa ggcacctgag cacaacacag ataacacctg ggcttatccg acgaatgcgg
    14761 aactggatgg cggtattcgt gaggcattcg atcacctgtt ccgcaccctg aaaacccgtg
    14821 agacggacaa gggcgttgcg ttttattccc gtaagggcgt gacccgcaca cctgaaggca
    14881 atctcatttc ggatgttaat cgcagtaagg aagccaaagg cagcccggtc ccgcaggttg
    14941 aagcggttgc ccgtggcgtg atgagcggca ttaaggacag tgacctgaag gtccgtgtgg
    15001 tgaagtcaca gaaagaggct gaagcgctgg cgggtgaatc gttcgacggt tacggcaggg
    15061 tgcacgcgtt ctatcgtccg gataaacgtg aaattgtcct ggtggcggat aacatccctg
    15121 acgggcggac cgttcgcgag aagctgcgtc acgagataat tcaccatgcc atggagcatg
    15181 ttgtcacgcc agcggaatat cagacgatta tcaaaaccgt gctgaaaacc cgcgacagtg
    15241 ataacgccac catccgtgaa gcatggcgta aggttgatgc gtcctatggt aaggaatcac
    15301 cggaagtaca ggcgggtgaa tttctggcac atatggcgga gaaacagccg aataaattcg
    15361 tggcggcatg ggagcgtgtt gttgccctgg tcaaaggggt actgcgtcgt acggggttac
    15421 tgaagccgac ggaactgaac gatatcagac ttgttcgcga gaccatccgt acgttaggcc
    15481 agcgtgtgcg ggaaggttac acgccgcgtg aggatggcgt gggcgcatcg tctcagtact
    15541 cccgtagtgg taagcctgat ccgttcaaag tgccggaagg tgagggcgag cgttatcgcg
    15601 atgaccttgc cagaatgatg aaatctctgc gtaccacaga tttaacggta aacatcgggc
    15661 gtacgccgcc ggtattgcgt caccttggcg caccggattt gccgctggtt atctcccgcg
    15721 atactgtgcg gaaggccacc aatggtgtga aacatgtggt gccgatggat gttatcgaga
    15781 gactaccgga actgatgcac gatccggatg caatttaccg ctcagcgaca gaaagaaatg
    15841 cggttgtgat gctgcttgat gccgtggata aaaatggtga tccggtggtg tcagcggtac
    15901 acatgaaggc tgtccggtcg cgtctggaaa tcaacaaggt agcttctgtt tacggtacag
    15961 aaaatggaaa aaaactgaag agtatggaaa tgaccgggtt aacgttgtac cggagagaaa
    16021 aattaagccg cgataacctt ctgcacagag ggctccaatt gcccaaaggg gaacattctt
    16081 atcgcggctc tgcggataaa atactctatc ctgaagatat tcgcaagggg ccgtattact
    16141 cccgtaccag cagtctgaca ccggaagaga ctattgcatc gcgttttgtg cgccagatgc
    16201 aggataaatt ccaggtgctg aaagctgttc aggagaatat ccgtaaaact ggcggcaaag
    16261 tggacgacag taacaacgct tatatggcag aagaactctt ccacgggaag gcggaaagcg
    16321 acctgaatgt gatgaaggag cgctacgttc agccactggc taaattactg gcggactaca
    16381 aaattgcgca ggccgatctg gatgagtacc tctacgcccg tcacgcgccg gaacgtaacg
    16441 cgcatatcgc gaaaatcaac ccgaaaatgc cggacggcgg ttcggggatg accaacgcgg
    16501 aggcggcgga aatcatgcag cgtgtgcgta acagcggcaa acaggcacag tatgaccgtc
    16561 tggcagggat tgttgacgat atgctggccc gtcgccgtga gcttatccgt gaggccggac
    16621 tggaagagag cggcgtggtg gatgcctggc agaacgccta ccgttactac gttcccctga
    16681 aaggccagga tgttgacggt gtggtgtcac ttccccgtac aggtaagggc ttcaccatcg
    16741 gcggacgtga aagcaggcag gccatggggc gtgcatcccg cgcacagtct ccgtccactc
    16801 aggcgataca ggacctgagc gaatcgctga tccgccatcg caaaaacgaa gtgggtaacg
    16861 ccttcctgaa actggtgcag gataatcctg acaaggatta ctggcaggta ttcaccgatg
    16921 acagaccgga taccatgcgg acgattgcag agcgcaagga ccaggaaact ggtgaaacca
    16981 ttcgcgaagt tgtcgagcgt ccggtggcga tggcaatgat ggcagaccgg tacttcacca
    17041 ccaaaaagaa cggcaaaacg tactacatca aactccatga cccgcgcctg atgcgtgcga
    17101 tgaagaatat gggaccggaa accagtaacg ccgtaatccg tacgctgggg aaagttaacc
    17161 gcttcctggc aacggtgaac acgtcgtata acccggaatt cctggtcagt aacttcatcc
    17221 gtgacgtgca gacggcggtg atgaacctga aggcggagca gggaaggagc gacggtaaac
    17281 tgaaagggct ggataactta tccgccctgg ctgtggtgaa agacagccgt tctgccatgt
    17341 cagccgtata cgccagtctg cgtggtaaaa ccctcacggg caaaggtgca cagtggcaga
    17401 aggtgtggaa agagtttgtt gaggacggag gtaaaaccgg ctggtttaac atgggtgacc
    17461 ttgaaggcca gcagaaggaa atggatcgcc ttgtttcgct ggcgaaggga ggatggaaag
    17521 gccagagtat cggtgcatgg aattcgttcc ttaaccttgt cgaggatgcc aacggggcgg
    17581 ttgaaaacgc tctgcgtctt tctgcctata aacacgcccg tgatgccggt ttgtcacgcc
    17641 agcaggcggc gtctcttgcc aaaaacatga cggtgaactt taaccgtcgt ggtgagcagg
    17701 gagcgctgat gaactcgctg tatatgtttg ccaacgccag cattcagggg accgcaaacc
    17761 tggtgagaac gctcggacat cttaatggcg acgggccgtt actggagcgc cttcgctgga
    17821 agaatctcaa tgtaccgcag aaaatcgcgc ttgccgctgt gggagcgggt tatctgcttg
    17881 gctcgcttaa ccgcagtgtt gcgggtgagg atgatgacgg ggttaactgg tatgacaagg
    17941 tgccgtctca tgtgaaagag cgtaacctcg tcattatgaa atcggtgttc gggggcaagg
    18001 ccggagagta ctggagtatt cccctgcctt acgggtacaa cgttttcttc ctgcttggtc
    18061 acacagctga aggtgtggcg gcgggtgacc tgacggcgtc ccgtgctgcc ggtaatgttg
    18121 ttggtggtgt gcttggtgca ttcagcccga ttggcagtga gacgtcggaa acactgtccg
    18181 gggcattgct gaaaaatgca gcgccgacca ttctgcgtcc gtttgcgaac cttgccatga
    18241 atgaaaactt catgggggcg cagatttacc aggagaacat gccgtttggt acaccaaaac
    18301 ctgacagcca gctgggaaga cgttcaacgc cagaagcgta caaggcgttt gcatcctggc
    18361 tgaatgcgtt ctctggtggc agccagtacc gtcccggcgc ggtggatatc acaccggaat
    18421 cgctgaaata ctgggtggac tatatctccg gagggacagg gcgcttcatt tccaaaacca
    18481 cggatgcggc ggtgaaatcg ctgaatggta ttgatatacc ggagcagcag gtgcccttcc
    18541 tggggaaaat ttcaggtgag gtgatgccgt atgctgacca gcagaagatg tacgaccgga
    18601 tgacagaggt tgcgcagtat cacgcagagc tgaagagtct gaccggtgca gaaagaacgg
    18661 cattcattga cgagaacaac ggaaaattgt cgatgaacgg gcttatgcag gatacccgga
    18721 agagactgaa ggatttgcgt aaacagcgtg atgccattta cgccgacagt actctcagtc
    18781 tggcgcaaca gtcggcgatg gtgaaatcgg tagagcggga tatgaaaatt gccgtggatc
    18841 ggtttaaccg cgagtacaac aaaaaagtgg gagtggagta aagaacatgc cccgtacgga
    18901 tgtacggggc tgatataaag tcacgcaggg aaatattgta gatgaactga acatttaaat
    18961 ttggataatg ttgtgatctt gatcagaaat tacagtgtaa gcagatgtga aatgtcatgc
    19021 ctgatgaggg taagattttt cttgtttcgg ttcctacgtt gctctatgtc tcaggagtga
    19081 atacaccaag aatatagtaa gataattcct ggtcatttaa aattaataaa cacgatcatt
    19141 ttcagaatat cctgaataaa gccgtaatta atcatatttc tttatgagta tgaggtgttg
    19201 cggctgattt gtatacagat aatttaacag gatatccata atgagtaaaa aatttacaaa
    19261 gacacttctg tcatcttctc tgagtgggct tctgcttggg atcagctcag gtgttttagc
    19321 tcaggagtta cctcctatca aagattctgg tttgcctttt tattcagtgc tgaaagaggc
    19381 agatacttcg tctgttatat acggtagtac ttatcgtatt tactataaca aatcctctgg
    19441 ttggggaaca atgcgatatg ttctgaacgg aaaagatgag acccttttta atttcgatca
    19501 ggagggtaat attatagtat tgagtaaaga taactcaatt gcatataccg ttcatgagcc
    19561 tgtgctaaaa gacttcgccc gtatggcagc aagcctcaga acgcctgaca aagaaaacgg
    19621 taagcatcag gttgatgagg aggaggtccg ccggatcttc aataaagtta ataatctatc
    19681 aaatactatt attaatcctg aagtattaaa acaatcgggt ttcatcgcat caggaccacg
    19741 tccggaaggt gaccgtttgg ttcaggctag tgcggcgggc gaactcgctc tggctgttgg
    19801 agaaggtgca gcggcggagg aaaagaattc aacagctgtt ggttcatggg ctagtgctca
    19861 tgggagacag agtactgcat taggaagtgg ggggcaggct tatgctgatg gttcaaccgc
    19921 gttgggaagt tattctcatg tggatgattc atccgtttat ggcgtggcat taggggacag
    19981 ctctgttgtg ggtgaaaatg cccccgaagg cactgcactg ggaagcagag cggaagtttc
    20041 tcatagaaat agtgttgctt taggagcgag agcgcacact acccgtgata atgaagttta
    20101 tatcggctac gtagaagaag cgggcaaggc ttataaaact cgtgtgcttg gtggcctgag
    20161 tgacggtact cgtccctcag atgcagcaac cgttcgtcag gttgaccgtg taaaagacag
    20221 tgttgagcaa ctggctcagg atacgaatac ccgccttgta gtcgaagcga aaaagtctcg
    20281 tgaatataca gactccagaa ccaccgttgg tgtgaatgcg gacggaaccc tgaccagcgc
    20341 tgatggcgct acaaaaacga ttgctgttaa tgacggtctc gttgctctgt ccggcagaac
    20401 agaccgtatt gatgctgcag ttggtagcgt tgaccgccgg gttactaaaa atacacaggc
    20461 tattcagtcc aatactcgcc agttgcagga acataatgca cgcctgaaca gccagcagcg
    20521 tcaaattcgt gagaatcacg aagagatgaa gcgtgcagca gcacagagtg cagcgctggc
    20581 ggggcttttc cagccgtaca gtgtggggaa attcaacgcc actgctgccc tggggggg
//
LOCUS       DAZGXZ010000091        20589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000091 DAZGXZ010000000
VERSION     DAZGXZ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.091"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(456..1652)
                     /gene="hemY"
                     /locus_tag="ACHKJF_003445"
     CDS             complement(456..1652)
                     /gene="hemY"
                     /locus_tag="ACHKJF_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP0950747.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVVLQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1655..2860)
                     /gene="hemX"
                     /locus_tag="ACHKJF_003446"
     CDS             complement(1655..2860)
                     /gene="hemX"
                     /locus_tag="ACHKJF_003446"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP0950748.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(2882..3622)
                     /gene="hemD"
                     /locus_tag="ACHKJF_003447"
     CDS             complement(2882..3622)
                     /gene="hemD"
                     /locus_tag="ACHKJF_003447"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP0950749.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3619..4560)
                     /gene="hemC"
                     /locus_tag="ACHKJF_003448"
     CDS             complement(3619..4560)
                     /gene="hemC"
                     /locus_tag="ACHKJF_003448"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP0950750.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4947..7493
                     /gene="cyaA"
                     /locus_tag="ACHKJF_003449"
     CDS             4947..7493
                     /gene="cyaA"
                     /locus_tag="ACHKJF_003449"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP0950751.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7533..7853)
                     /gene="cyaY"
                     /locus_tag="ACHKJF_003450"
     CDS             complement(7533..7853)
                     /gene="cyaY"
                     /locus_tag="ACHKJF_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP0950752.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7901..9226)
                     /locus_tag="ACHKJF_003451"
     CDS             complement(7901..9226)
                     /locus_tag="ACHKJF_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP0950753.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            complement(9223..10611)
                     /locus_tag="ACHKJF_003452"
     CDS             complement(9223..10611)
                     /locus_tag="ACHKJF_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP0950754.1"
                     /translation="MYQKQGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLD
                     KWDKVPPLTDLTTDYLYEGAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFK
                     AWGDFAADKHSLYFEGKRTDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLY
                     INGHRLADPDSFRVLAQKSWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQL
                     DADPDTFSVVRWMPGSLLTWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFW
                     RKGPACKQEELPGLDPEQFQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLII
                     NKRFNAGKRHGYLLTRAEGWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRR
                     WYARDNRYIYSFDGAQLWRYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDG
                     EFIPAPRNEATK"
     gene            complement(11069..11623)
                     /locus_tag="ACHKJF_003453"
     CDS             complement(11069..11623)
                     /locus_tag="ACHKJF_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP0950755.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            11973..12176
                     /gene="yifL"
                     /locus_tag="ACHKJF_003454"
     CDS             11973..12176
                     /gene="yifL"
                     /locus_tag="ACHKJF_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0950756.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            12213..13037
                     /gene="dapF"
                     /locus_tag="ACHKJF_003455"
     CDS             12213..13037
                     /gene="dapF"
                     /locus_tag="ACHKJF_003455"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP0950757.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            13034..13741
                     /gene="yigA"
                     /locus_tag="ACHKJF_003456"
     CDS             13034..13741
                     /gene="yigA"
                     /locus_tag="ACHKJF_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFP0950758.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            13738..14634
                     /gene="xerC"
                     /locus_tag="ACHKJF_003457"
     CDS             13738..14634
                     /gene="xerC"
                     /locus_tag="ACHKJF_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP0950759.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            14634..15350
                     /gene="yigB"
                     /locus_tag="ACHKJF_003458"
     CDS             14634..15350
                     /gene="yigB"
                     /locus_tag="ACHKJF_003458"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP0950760.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            15434..17596
                     /gene="uvrD"
                     /locus_tag="ACHKJF_003459"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             15434..17596
                     /gene="uvrD"
                     /locus_tag="ACHKJF_003459"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP0950761.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(17743..18507)
                     /gene="yigE"
                     /locus_tag="ACHKJF_003460"
     CDS             complement(17743..18507)
                     /gene="yigE"
                     /locus_tag="ACHKJF_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP0950762.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            18669..18725
                     /gene="ysgD"
                     /locus_tag="ACHKJF_003461"
     CDS             18669..18725
                     /gene="ysgD"
                     /locus_tag="ACHKJF_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP0950763.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            18877..19827
                     /gene="corA"
                     /locus_tag="ACHKJF_003462"
     CDS             18877..19827
                     /gene="corA"
                     /locus_tag="ACHKJF_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP0950764.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19869..20360)
                     /locus_tag="ACHKJF_003463"
     CDS             complement(19869..20360)
                     /locus_tag="ACHKJF_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP0950765.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(20357..>20589)
                     /locus_tag="ACHKJF_003464"
     CDS             complement(20357..>20589)
                     /locus_tag="ACHKJF_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFP0950766.1"
                     /translation="QKISLLSFQNYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRF
                     FRIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
BASE COUNT         4854 a         5262 c         5314 g         5159 t
ORIGIN      
        1 ttttttagtt ttaacggcaa ttaatatatg tgttattaat tgaatgaatt ttatcattca
       61 taataagtat gtgtaggatc aagctcaggt taaatattca ctcaggaagt tattactcag
      121 gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt atttcccgat
      181 tctctgtggc ataataaacg ggtagatgct cattccatct cttatgttcg ccttagtgcc
      241 tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact gacagatgtc
      301 gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac ttgttgtcat
      361 acagacctgt tttaacgcct gctccgtaat aagagcaggc gtttttttat gcatcagaaa
      421 ggtcccggag gtgcttgcct ccgggtgaga aggaactact gtggtgggtt gttctgcaac
      481 gttaacatca aaccgtcgcg acgcatcgct gcagcttctt ccggcttgtg cagtctgtcc
      541 agcgcgtcgg caagccatgc gtaatcgtag gcgtccggac gttgtttcag cgccgcgcgg
      601 aaggcgagcg atgcttcctg ccattctccg tgcttcatca gtgactggcc cagtgtgctc
      661 cacaacagcg ggcgatcgcc gacgtttttg atctgctggc gcagcacttt ttccagctgt
      721 tccggattgt tggttttcag tcgaggaatc ggcagcagta ggcgatcgtc gtactggcgt
      781 ttcaggccat cgatgataat ttgctgggca gtatcatgat cgtcacattc aataagatgt
      841 tccgccattg ccacctgcaa cactacctga tgacgcgttt tccggctttg gtttttccac
      901 cagttacgca aaccttcact accgttatcg gcacgcgcct gatccatcag gccaatccat
      961 gcctgttgtt ccagcattgc acgatgttct tcatcaccaa catgggcttt cgccattgat
     1021 gggatgatat ccagcagaga actccatgca cctgtgcgga tatacgcctg ttccgccaga
     1081 cgtaatactt ccggatggcg tggcgtaact tccagcagct tatccacgcc gtggcgtgca
     1141 gcatggtttt cattacgggc cagttgcaga cgtacacggg tgatttctac cggaatggtg
     1201 tcattgccgg ccagctctgc tgcgcgttcc agatgttggt tggcgcgtgc ttcatcacca
     1261 cgttgttgcg cggcttcggc agccagtaga tagttcacca ccggttgttc cgcgtgatcg
     1321 gcatttttcg ccatcagctt ttcaacttgc tgataatcgc cttccgccag tttcagcagc
     1381 gcctgttcgg tctgcttacg ggcacggcga cgcttacgtc cgacaaacca cccacgggtg
     1441 tgtgcgcctg tgcggaagat ccgccgcagt agccactcaa tggcaaacag cactaccatc
     1501 gccagaatca aaataatcgc caggcccgtg acgctggttt cgatattgta gttgtcagtc
     1561 tggatcagca cgtaaccctg atgaccggca atcatcgggc caaccactat ccccgcaatc
     1621 agcaaaacaa agagtaataa cacttttagc atggttattc tccttgcggc gcggctgccg
     1681 gagtatcacc ttgcggtgca ggtgcaggtg caggtgcagg tgcaggtgca ggtttagctt
     1741 ccgttgtccc cgctgccggt tgtgccagca ggttacgcac gcgggtctgc atcagttttt
     1801 ccagcatcgc ctggctttgc agggtttccg gaagatccat cgagatattt tgctggctta
     1861 actggtccac ctcgtcgagg aacgctttgg tggtggcatc atcagtatcg tagtaagctc
     1921 gtacccaggt ggagacgttc tccagcgcct ggcgataagt ctcttcctgg tgacgcggta
     1981 cagcttgtgc tgcgaccagc aggcgagagc gaatattttc gcgcagatag atatcctgat
     2041 ttggcgctaa cagcggtacg gcggtgtcat cacgacggcg aatcgtaatg aagttgtcca
     2101 taaagttctg ccagcttttt tgcagattga tacgccattc gctgatggaa ctggaaagct
     2161 cttcaccgtc ggaatccatc ggcgaaccat cgctgtcatt atcggccaga cgcaggttat
     2221 ctacctgatt tgaaagctga ttaagcttaa ggatgatgcc gtcataatcc acctgcgaaa
     2281 ctgcagaaag gctggcgata tcatcggtaa ttgcccgacg aacggtaatc agactcgggt
     2341 cattcatatc cgccaggctg gcatctgcac ttttcagcaa cgctgcagcg gtcgtgacgt
     2401 cctgatcgct ccacagcttc cgtccggcga gtttcaccag aaaatcggcc tgagccagca
     2461 gccaggtttt agcatcgctg ccggaaatgg tggcgacctt ttgttggact tcatccaact
     2521 gttttgccag cgtttcttgc tgacgattcg cctgcttaag ctgtgcagct tgttgcttaa
     2581 taatgccttc cagctcggct ttttggctct cctgggcttt ttgcaatgcc gtcagttggt
     2641 tagccagggc atcgctggtg gcggtctgat tgacggcctg ttgtttaccc cagccataca
     2701 aaccgacgcc cgccgccaga gcaatagcga tagccaccgc gctgagaatc aatgcggtat
     2761 tgttcttact ctttttttct gttgcgacag gttgtgacgt ggtgtccacg gcctccctgg
     2821 tctcttcaac cacggcggag gttttttctt gttccgtcat tatggcttcc tgttatgaga
     2881 gttattgtaa tgcccgtaaa agcgcatcgt tgtcagcgtt atcggcgacc ttaatgtctt
     2941 gccagcccag ttcccgggcg agtttcgcca aacgctcact gacgaccaat agtcgacagt
     3001 gtagtaacca gtgctcacga taccattgtg ggatcagcga ccagagttgc tgcaacattt
     3061 caccgctggt aacaacgacc atcgtcacct cgcgggattg ccagcgcatc gcttcttctg
     3121 caccatcgta atggattgcg catcgttgat aacattcaca aaaagtgacc tcagcaccgc
     3181 gcgctgtcag ggtatcccca attagctcac gaccgccatt gccacgtaat atcagcgcac
     3241 gtttgcccgc aatattttgt aattcaggta attgtagcaa gacttcgctg atttcccgat
     3301 cctgcgggta gagaatcttc tgtccgctta cggtatgtag cgccagtgcg gtggtgcgtc
     3361 caatggcgaa ataatcaggt agtcggggcc atttacgatc ttgctgatgc agctgtgatt
     3421 gggcaaaagc aaccgcgtgt tgcgagaggg caaacaacag atcgctctcc cccagcgccg
     3481 ccagttgatc agcaagttgc ggtaattgtc gacccggaga aaactcaatc agcggaaaat
     3541 gccaggccac ctgccccagt gtgcgcagac ggctcactaa ctcttctcca gcgggagacg
     3601 ggcgggtgac caggatactc atgccggggc gtctccgtta tagacttcag cgaggatctc
     3661 gcgcgcgccg ttattcagta gctcttctgc cagcgaaatc cccatttgtt cggcattttg
     3721 cggcgcaccg cggcgttcac cgcgaataat ctgcgaaccg tccggcgcgc cgaccagcgc
     3781 acgcagccag atttcaccat caataagctc ggcgtagcta ccaattggca cctgacatcc
     3841 accttcgaga cgggtattca tggcgcgttc tgcggtaacg cgcagtgcag tttcgtggtg
     3901 attcagcgcg gcaagcagct cgcgagtgcg cgtatcatca aggcggcatt caataccgac
     3961 tgcaccttgt cctaccgccg gaagagaaat ctcgggtggt aacgcggcgc gaatgcggga
     4021 ctccagacct aaacgtttta gtccggctac ggcaagaatg atggcatcgt attcgccgtt
     4081 atctagtttg ctcaggcgag tgccgacgtt gccgcgcagg gagcggataa tcagatccgg
     4141 gcggcgttca gccagttggc actggcgacg taaactggac gtcccgacga tactgcctgc
     4201 cggtaacgca tccagactgt cataattatt ggacacaaag gcatcgcgag gatcttcacg
     4261 ctcacaaata gtgaccaacc ccagaccttg cgggaattca accggcacat ctttcattga
     4321 atgtacggcg atatcggcgc gattctccag gagcgcgact tccagctctt ttacaaataa
     4381 gccttttccg cctactttcg ccagcggcgt atcaagaatc acatcgccgc gcgtcaccat
     4441 cggtaccagt tcaaccacca ggcccggatg gctcgccatc aacttgtctt tgacatagtg
     4501 tgcctgccag agtgcaagtg ggctttggcg tgtggcaatt cttaaaacat tgtctaacat
     4561 gcttgttacc gtcattatca tccgtggtcc atcctaacat ccttgccaga gtgatgtcag
     4621 tgttgtggtg aaacgtagac gcctgcgcaa accgtaaaat gaggtctggc agtggatcct
     4681 gacaggcgtt tcacgccgtc gtaataagga atttacagag aataaacggt gctacacttg
     4741 tatgtagcgc atctttcttt acggtcaatc agcaaggtgt taaattgatc acgttttaga
     4801 ccattttttc gtcgtgaaac taaaaaaacc aggcgcgaaa agtggtaacg gttacctttg
     4861 acatacgaaa tatcccgaat gccgcgtgtt accgttgatg ttggcggaat cacagtcatg
     4921 acgggtagca aatcaggcga tacgtcttgt acctctatat tgagactctg aaacagagac
     4981 tggatgccat aaatcaattg cgtgtggatc gcgcgcttgc tgctatgggg cctgcattcc
     5041 aacaggtcta cagtctactg ccgacattgt tgcactatca ccatccgcta atgccgggtt
     5101 accttgatgg taacgttccc aaaggcattt gcctttacac gcctgatgaa actcaacgcc
     5161 actacctgaa cgagcttgaa ctgtatcgtg gaatgtcagt acaggaccca ccgaaaggtg
     5221 agcttccaat tactggtgta tacaccatgg gcagcacctc gtccgtaggg caaagttgtt
     5281 cctctgacct ggatatctgg gtctgtcatc aatcctggct cgatagcgaa gagcgccagt
     5341 tactacaacg taaatgtagt ctgctggaaa gctgggccgc ctcgctgggt gtggaagtca
     5401 gcttcttcct gattgatgaa aaccgcttcc gtcataatga aagcggcagc ctggggggcg
     5461 aagattgtgg ctccacccag catatactgc tgcttgacga attttatcgt accgccgtgc
     5521 gtctcgccgg taagcgtatt ctgtggaata tggtgccgtg cgacgaagaa gagcattacg
     5581 acgactatgt gatgacgctt tacgcgcagg gcgtgctgac gccaaatgaa tggctggatc
     5641 tcggtggctt aagctcgctt tctgctgaag agtactttgg tgccagcctt tggcagctct
     5701 acaagagtat cgattcccca tacaaagcgg tactgaaaac actgctgctg gaagcctatt
     5761 cctgggaata cccgaaccca cgtctgctgg cgaaagatat caaacagcgt ttgcacgacg
     5821 gcgagattgt atcgtttggt ctcgatccat actgcatgat gctggagcgt gttactgaat
     5881 acctgacggc gattgaagat tttacccgtc tggatttagt acgtcgctgc ttctatttaa
     5941 aagtgtgcga aaagctcagc cgtgaacgcg cctgcgtagg ctggcgtcgc gcagtgttga
     6001 gccagttagt gagcgagtgg ggttgggacg aagctcgtct ggcaatgctc gataaccgcg
     6061 ctaactggaa gattgatcag gtgcgtgagg cgcacaacga gttgctcgac gcgatgatgc
     6121 agagctaccg taatctgatc cgctttgcgc gtcgcaataa ccttagcgtc tccgccagtc
     6181 cgcaggatat cggcgtgctg acgcgtaagc tgtatgccgc gtttgaagca ttaccaggta
     6241 aagtgacgct ggtaaacccg cagatttcac ccgatctctc ggaaccgaat ctgaccttta
     6301 tttatgtgcc gccgggccgt gctaaccgtt caggttggta tctgtataac cgcgcgccaa
     6361 atattgagtc gatcatcagc catcagccgc tggaatataa ccgttacctg aataaactgg
     6421 tggcgtgggc atggtttaac ggcctgctga cctcgcgcac ccgtttgtat attaaaggta
     6481 acggcattgt cgatttgcct aagttgcagg agatggtcgc cgacgtgtcg caccatttcc
     6541 cgctgcgctt acctgcaccg acaccgaagg cgctctacag cccgtgtgag atccgccatc
     6601 tggcgattat cgttaacctg gaatatgacc cgacagcggc gttccgcaat caggtggtgc
     6661 atttcgattt ccgtaagctg gatgtcttca gctttggcga gaatcaaaat tgcctggtag
     6721 gtagcgttga cctgctgtac cgcaactcgt ggaacgaagt gcgtacgctg catttcaacg
     6781 gcgagcaatc gatgatcgaa gccctgaaaa ctattctcgg caaaatgcat caggacgccg
     6841 caccgccaga tagcgtggaa gtcttctgtt atagccagca tctgcgcggc ttaattcgta
     6901 ctcgcgtgca gcaactggtt tctgagtgta ttgaattgcg tctttccagc acccgccagg
     6961 aaaccgggcg tttcaaggcg ctgcgcgttt ctggtcaaac ctgggggttg ttcttcgaac
     7021 gcctgaatgt atcggtacag aaactggaaa acgccatcga gttttatggc gcgatttcgc
     7081 ataacaaact gcacggcctg tcagtgcagg ttgaaaccaa tcacgtcaaa ttaccggcgg
     7141 tggtggacgg ctttgccagc gaagggatca tccagttctt tttcgaagaa acgcaagacg
     7201 agaatggctt taatatctac attctcgacg aaagcaaccg ggttgaggtg tatcaccact
     7261 gcgaaggcag caaagaggag ctggtacgtg acgtcagtcg cttctactcg tcatcgcatg
     7321 accgttttac ctacggctca agcttcatca acttcaacct gccgcagttc tatcagattg
     7381 tgaaggttga tggtcgtgaa caggtgattc cgttccgcac aaaatctatc ggtaacatgc
     7441 cgcctgccaa tcaggatcac gatacgccgc tattacagca gtatttttcg tgatgaacgt
     7501 gccggaaagc gaggcttatc cggcatgcaa tcttagcgga aactgactgt ttcacccgcc
     7561 tgctgcgtcg ccgcctgttc cagcaaatcc cagaaggttt cgccgctgcg atcgcaaatc
     7621 cactcatcgc cttttaggtc aaaatggtag ccgccctgtt tggttgccag ccacacctgg
     7681 tgcagcggct cctggcggtt aataatgatt ttgctgccgt tttcaaaggt aatggtcagt
     7741 acgccgccgt tgatttcgca gtcgatatcg ctgtcgccat cccagtcgtc caggcgttct
     7801 tcaatggtca gccagagttg atcagccagg cgatgaaatt cactgtcgtt cattgttgta
     7861 tcctgtcttt aagtgatggc ggcagtatag cggtatgggg tcagggcttc aaagtttgcc
     7921 cgtctgttgc tgcgttccgg catgagtcat ccgtcaacgg aataataatg tctctgttgt
     7981 gaagttcacg cacattctct tctctgacgt gtgttttcgc cgggcagggg gagggcgtaa
     8041 tccttacctc tgtatcggtc catgtataaa cattgcgatc gtcataagcg aagcgatcgt
     8101 ttttagttag cggaccgtta actggaataa tttgcacgct ctttgaccgt aattggcgga
     8161 taaaataagc atgctgtttg ccgctattaa atgagcgatc aatgaccagg tctggcgtat
     8221 ccagcgggat tatctcaagc tggtcttcac caaatgtcga taattttgtt acatacaacc
     8281 gatctttata ttgcgcaata ttgccagtaa tcagatggaa ttgttcaggt tcaatatctg
     8341 gtaggtctat ccacttgctc cactgacggt cggacgtgag ctgtttacgc cagcgaacgc
     8401 gactttcccc tacttcaaaa gagacgtcat catctacggg tatcgctttg ccagacaatt
     8461 gcccaaaggg ataacgatgc atacccttat tgtcgcgaaa aaccagcagt gaatgaggta
     8521 tccagcgaat tatctcaaat gagtctgcat tcgccgtaag gtgaacgcca tttaaataaa
     8581 tattctgccc actgcggata agcagatcat tggaatacaa atttctgttt acactcataa
     8641 gacgtggatt aataccccac catctttttt ctaatacagt aacattactg ctgctcccct
     8701 gacgtcgacc ggacaaatag agatttttac tatccaccag cgtggtagag ttaccttcga
     8761 tagcttttag tgtcgccaga tcaacacgtg acgcgccgct attactttcg gtgcgctgtc
     8821 cgtcaaaata gaggctatat ttatcgaccg cgaagcggcc catagcctga aagctggcaa
     8881 tatcgacagt gggtgttccc ggtggattgc taacgatttt gccacgccag agaataacgc
     8941 ggccgtctgt cacccagtgg tattcctcat tttttatgct tatttcatcg gtgggtatat
     9001 agccttccac caactcccgc tccccggata tatttgtcac aaggcgcaaa tcggctctgt
     9061 tcatcttcgg aaattgggca atgattttct tatcccccca ataaccttta ccgtaagaca
     9121 taacaccgtg atcattgtaa tagtcgaacc ctatggcctg tacagcaggc gtcagaaatg
     9181 tgtgtcccaa caggatgagt gcaactgtga ttatttgacc cttcattttg tcgcctcatt
     9241 gcgcggggca ggaataaatt cgccgtcatc agtaattttt ccgtccagat atcccgtttt
     9301 gtaatttacg ccatagccat atcccgaatg ttgttccttc cagattaagc gaacttgttt
     9361 cggatcagcg gtgcggtatc gccagagctg cgcgccatca aagctgtaaa tgtagcggtt
     9421 atcgcgggca taccatctgc gcaaaaaggg atggtcatta agatgggctt cgcgttcatc
     9481 cggagaacgg ttatccagta agatcagcgg tccgtcagat tcaaacacat gtaaaccgga
     9541 atggtatggc cacccttcgg cacgcgtaag taaatagccg tggcgtttcc cggcgttgaa
     9601 acgtttgttg ataataagat tgggatcatc aagtttcacg atttccagct tgcggtcacc
     9661 agactctgtt tcgatgatgg tataaagcga gtcctgatac tgggcgacag catcactgat
     9721 gggttgaaac tgctccggat cgagtccggg taattcttcc tgtttacaag caggtccttt
     9781 acgccagaac actttctttt ccagcagaga aaaatccaga cagtgctttg ttaaatcttc
     9841 atcccactcc agattttcct tgtcgaggac tttacgctgc agcccgtttt tatcacgcca
     9901 ggtcaagagt gagccgggca tccagcgcac gacggaaaag gtgtccgggt cggcatcaag
     9961 ctgttcgccg ttgatcagga ttttggtccg tgtacgagcc agggtatccc aggggccaaa
    10021 tggcatagcc acacagggat taaacgcggt agaaaacttt cctcgctgat cccatgactt
    10081 ttgtgccagc acacggaaac tatcgggatc tgcgaggcga tgcccattga tatacaagaa
    10141 attagcgtca cgtaatatca gccccagtaa gtcaggatcc cagggaggcc ggaattccac
    10201 ctgatgcagt gttttgatat ctaagcgatt tcccccgcca ttgttatcgg tgcgcttgcc
    10261 ttcgaaatag agactgtgtt tatctgcggc aaaatcaccc cacgccttga acgaggcgac
    10321 atcgacggga ggtttgtctg gcgggttttg cacaacttca cccgcgtaga ggatatagcg
    10381 accatcgcta tgccaggagt aatgaggata ccaggcctgt gcgccttcat agaggtagtc
    10441 ggttgtcaga tcagtcagag gaggtacttt atcccatttg tctagtgttg tcgggtctat
    10501 taataaattg ggtaaacggc gcaaactttt gtaattcacg tcaggaagag aaagatttgt
    10561 ttctttataa gggcgcaggt ataccacgcc ttttccctgt ttctggtaaa tattatgttc
    10621 aggcggatca agccgacatc cccatgccga cgataaaaac tatgaacaag acaacaaaca
    10681 aaccacattg cgatagtgca taaagccatc ctggcgcgag gtgccgatca cgaaactacc
    10741 agcagaacat aattcctcgc acgtaagcgt aacttttgcg gcaaatcgtc acttttcaga
    10801 atctataaag gccatcgcta ttttctgata gttgctgggg taaacttgag gttttcattc
    10861 acgatataaa tacgagctac tgcgccatgg tgtgactgat cacgttaaaa tgtgccggat
    10921 tgacgccggg aagggtgtca acatgcgact ctgcccaccg ttacctactt tattgtcttt
    10981 cgcgtgttgt acttaggtcc caccaccggt atctaacatt ccccacatta ttacgctttc
    11041 tgccgaacgt gatttttata gaaatctatc aattcatttt ccctgtacca taaagtttat
    11101 ctttcacatc ctgccgaacg cgggtcttgt accccccgtt gtgaagatct tcatacggag
    11161 aaaaagaatc tttactccac cccagcagca ctttatcgcc aggcataaaa tgcacatgca
    11221 gaaaatcttc tccccattct cttttgggga gaggtaacgt catattccgt ttctccagac
    11281 gcattccagc attatactgt gtacgagtag tacttaatgt ccagataacc tctgccgtat
    11341 ccccactaac atcaccacaa caggttgttg aaccgccacc atttgcaaaa gcattcgcgc
    11401 ccgccacgcc gttaacactg aatcccagta tcgggcggtc aatttcactg tggatttcca
    11461 gaatcactcc acctcttggt cctccccata ctgcctgtcc tatattccac aacacgacca
    11521 gtaccggaac gacaattaac gtccgccata cccagcgccc ccaccgggca taaccgcgcg
    11581 taagcttttg gtcaaatttt tccagttggg taaatagccc catcagattt tattccctct
    11641 tatcaccagg ttggcaaagt tgtatgtcac tggcgactaa ggtcagacaa cgaaattgca
    11701 tggtgcgcca ctctattaaa agccccatgc cgacgataag aacaatgaac aagacaacaa
    11761 acaaaccaca ttgcgatagt gcataaagcc atcctggcgc gaggtgccga tcacgaaact
    11821 accagcaaaa cataaatccc cacgtgtaag cgttatactc gcagcatttc ctcacttttc
    11881 agacttcata aagagtcgct aaacgcttgc ttttacgcct tctcctgcga tgatagaaag
    11941 cagaaagcga tgaactttac aggcaatcca taatgaaaaa cgtgtttaag gcactcactg
    12001 tattaattac tctcttcagc ctgacgggct gcggtctgaa aggtccgctc tatttcccgc
    12061 ctgcagataa aaacgcaccg ccgccgacca aaccggtaga gacgcaaacg caatccacgg
    12121 tgccggataa aaacgatcgc gccactggcg atggtccatc ccaggtgaat tactaaaagt
    12181 cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt ctcgaaaatg catggccttg
    12241 gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt ctttttttca ccggagctga
    12301 ttcgtcgcct ggctgatcgg cacctgggag tagggtttga ccaactgctg gtggttgagc
    12361 cgccgtatga tcctgaactg gattttcact atcgcatttt caatgctgat ggcagtgaag
    12421 tggcgcagtg cggcaatggt gcgcgctgct ttgcccgttt tgtgcgtctg aaagggctga
    12481 ccaataagcg cgatatccgc gtcagcaccg ccaacgggcg gatggttctg accgtcaccg
    12541 atgatgatct ggtccgcgta aatatgggcg aacccaactt cgaaccttcc gccgtgccgt
    12601 ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc cgccgagcag acaatcttat
    12661 gcggcgtggt gtcgatggga aatccgcatt gcgtgattca ggtcgatgat gtcgataccg
    12721 cggcggtaga aacgcttggt cctgttctgg aaagccacga gcgttttccg gagcgagcca
    12781 atatcggttt tatgcaagtg gttaagcgcg agcatattcg tttacgcgtt tatgagcgtg
    12841 gggcaggaga aacccaggcc tgcggcagcg gcgcatgtgc ggcggtggcg gtagggattc
    12901 agcaaggttt gctggccgaa gaagtacgcg tggaactccc cggcggtcgt cttgatatcg
    12961 cctggaaagg tccgggtcac ccgttatata tgactggccc ggcggtacat gtctacgacg
    13021 gatttattca tctatgaagc aaccagggga agaactgcag gaaacactca cggagcttga
    13081 tgaccgggcg gttgtcgatt atctgattaa aaatcctgag ttttttatcc gtaatgcgcg
    13141 cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggc accgtttcgt tggtcgagtg
    13201 gcacatggcc cgcgcacgta atcatattca tgttctggaa gagaacatgg cgctgttgat
    13261 ggaacaggct atcgccaacg aaggcctgtt ttatcgccta ctctacctgc agcgcagtct
    13321 caccgccgcc agcagtctcg acgatatgct gatgcgcttt caccgctggg cgcgcgatct
    13381 cggcctggca ggtgcgagtc tgcgcctgtt tccggatcgc tggcgcttag gtgcgccgtc
    13441 gaaccacact catctggcat taagccgtca gtctttcgaa ccgctgcgta ttcagcgttt
    13501 ggggcaggaa cagcactatc ttgggccgct taacggacca gagctgctgg tggtgctacc
    13561 ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg ggaagcgatg ctgatttggg
    13621 tgttgtgctg tttaccagtc gcgatgccag tcactatcaa caagggcaag gaacgcagtt
    13681 acttcatgaa attgcgctga tgttgccgga gcttctggag cgttggattg aacgcgtatg
    13741 accgatttac acaccgatgt agaacgctac ctacgttatc tgagcgtgga gcgccagctt
    13801 agcccgataa ccctgcttaa ctaccagcgt cagcttgagg cgatcatcaa ttttgccagc
    13861 gaaaacggcc tgcaaagctg gcagcaatgt gatgtgacga tggtgcgcaa ttttgctgta
    13921 cgcagtcgcc gtaaagggct gggagcagca agtctggcgt tacggctttc tgcgctacgt
    13981 agcttttttg actggctggt cagccagaac gaactcaaag ctaacccggc gaaaggagtt
    14041 tcggcaccga aagcgccgcg tcatctgccg aaaaacatcg acgtcgacga tatgaatcgg
    14101 ctgctggata ttgatatcaa cgatcccctc gctgtacgcg accgtgcaat gctggaagtg
    14161 atgtacggcg cgggtctgcg tctttccgag ctggtggggc tggatatcaa acacctcgac
    14221 ctggagtctg gtgaagtgtg ggtaatgggg aaaggcagca aagagcgccg cctgccgatt
    14281 ggtcgcaacg ctgtggcgtg gattgagcac tggcttgatt tgcgcgacct gtttggtagc
    14341 gaagacgacg cgctttttct gtcgaaactg ggcaagcgta tctccgcgcg taatgtgcag
    14401 aaacgctttg ccgaatgggg cataaaacaa gggctgaata atcacgttca cccacataaa
    14461 ttacgtcact cgttcgctac gcatatgctg gagtcgagcg gcgatcttcg tggtgtgcag
    14521 gagctgctgg gtcatgccaa cctctccacc acgcaaatct atactcatct tgattttcaa
    14581 caccttgcct cggtgtacga tgcggcgcat ccacgcgcca aacgggggaa ataatgcgtt
    14641 tttaccggcc tttggggcgc atctcggcgc tcacctttga cctggatgat accctttacg
    14701 ataaccgtcc ggtgattttg cgcaccgagc gagaggcgct tacctttgtg caaaattatc
    14761 atccggcgct gcgcagcttc cagaatgaag atctgcaacg cctgcgccag gcggtacggg
    14821 aagcggaacc cgagatttat cacgacgtga cgcgttggcg ttttcgttcg attgaacaag
    14881 cgatgctcga cgccgggctg agtgccgaag aagccagtgc aggcgcacac gcagcaatga
    14941 ttaactttgc caaatggcgc agccgaatcg acgtcccgca gcaaactcac gacaccttaa
    15001 aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa cggtaacgcc cagccggaac
    15061 tgtttggttt gggggattat tttgagtttg tgctgcgcgc tggcccgcac gggcgctcaa
    15121 aaccgttcag cgatatgtac tttttggctg cggaaaaact caacgtgccg atcggcgaga
    15181 tcttacatgt tggggacgat ctcaccactg acgtgggtgg ggcaattcgc agcggaatgc
    15241 aggcttgttg gatcagaccg gaaaatggcg atctgatgca aacctgggac agccgtttac
    15301 tgccgcatct ggaaatttcc cggttggcat ctctgacctc gctgatataa tcagcaaatc
    15361 tgtatatata cccagctttt tggcggaggg cgttgcgctt ctccgcccaa cctattttta
    15421 cgcggcggtg ccaatggacg tttcttacct gctcgacagc cttaatgaca aacagcgcga
    15481 agcggtggcc gcgccacgca gcaaccttct ggtgctggcg ggcgcgggca gtggtaagac
    15541 gcgcgtactg gtgcatcgta tcgcctggct gatgagcgtg gaaaactgct cgccatactc
    15601 gattatggcg gtgacgttta ccaacaaagc ggcggcggag atgcgtcatc gtatcgggca
    15661 actgatgggc accagccagg gcggcatgtg ggtcggcacc ttccacgggc tggcgcaccg
    15721 cctgctgcgt gcgcaccata tggacgccaa tctgccgcag gatttccaga tcctcgatag
    15781 cgaggaccag ctgcgcctgc ttaagcgtct gatcaaagcc atgaacctcg acgagaagca
    15841 gtggccgccg cgccaggcaa tgtggtacat caacagccag aaagatgaag gcctgcgtcc
    15901 gcatcatatt caaagctacg gtaatccggt ggagcagacc tggcagaagg tgtatcaggc
    15961 gtatcaggaa gcgtgtgacc gcgcgggcct ggtggacttc gccgagctgc tgctgcgcgc
    16021 tcacgaactg tggcttaaca agccgcatat cctacaacac taccgcgaac gttttaccaa
    16081 tatcctggtg gacgaattcc aggataccaa caacattcag tacgcgtgga tccgcctgct
    16141 ggcgggcgac accggcaaag tgatgatcgt cggtgatgac gaccagtcaa tctacggctg
    16201 gcgcggggcg caggtggaga atattcagcg tttccttaat gatttccccg gtgccgaaac
    16261 tattcgtctg gagcaaaact accgctctac cagcaatatt ctgagcgccg ctaacgccct
    16321 gattgaaaac aataacgggc gtctgggtaa aaaactgtgg accgatggcg cggacggtga
    16381 gcctatttcc ctctattgcg cttttaacga actcgatgaa gcgcgttttg tggttaaccg
    16441 catcaaaacc tggcaggaca acggcggggc gcttgccgag tgcgccattc tctaccgcag
    16501 taacgcccag tcgcgtgtac tggaagaagc actgttgcag gccagtatgc cgtaccgtat
    16561 ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa gacgcgctct cgtatctgcg
    16621 tctgattgcc aaccgtaacg acgacgcggc gtttgagcgc gtggtgaata cgccaacgcg
    16681 gggtattggt gaccggacgc tggacgtggt acgtcagaca tcgcgcgatc gccagttaac
    16741 actctggcag gcatgtcgtg aactgttgca ggaaaaagcc ctcgccgggc gagctgccag
    16801 cgccttgcag cgatttatgg aattaatcga tgccttagcg caagaaactg ccgatatgcc
    16861 gctgcatgta cagactgacc gggtaattaa agactccggc ctgcgcacca tgtacgagca
    16921 ggagaagggc gaaaaaggcc agacgcgtat cgaaaactta gaggaactgg tgacggcaac
    16981 gcgccagttc agctacaacg aagaagatga agatttaatg ccgctgcagg cattcctctc
    17041 ccatgcggcg ctggaagcgg gcgaggggca ggcggatacc tggcaggatg cagtgcagtt
    17101 gatgacgctg cactcggcga aaggcctgga gttcccgcag gtgtttattg tcggtatgga
    17161 agagggtatg ttcccaagcc agatgtcgct ggatgaaggc gggcgtctgg aagaagaacg
    17221 ccgtctggcc tacgttggcg taacccgcgc gatgcagaaa ctgacgctga cctacgcgga
    17281 aactcgccgt ctgtatggca aagaggttta ccatcgcccg tcgcgcttta ttggcgagct
    17341 gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg gtaagccgcc cggtcagcca
    17401 tcagcggatg ggtacgccga tggtcgagaa cgacagcggc tacaagctcg gccagcgcgt
    17461 acgccacgct aagtttggtg aaggcaccat tgtcaatatg gaaggcagcg gtgagcatag
    17521 ccgtttgcag gtggcatttc agggccaggg tattaaatgg ctggtggcgg cttatgcccg
    17581 gctggagtcg gtgtaacgtt gccggatgcg gtgctgcgca ccttatttgg cctaaaaaat
    17641 cattcagatt caataaattg caacgtcatg taggccggat aaggcgttta cgccgcatcc
    17701 ggcatcagca gcatatttag tatctgacac aaaactatca ttctaacctt tccgctcgac
    17761 ggaaatcatg gtgacaaaag gatagcgttg ccacgggatt gccccgcctt tcatatacat
    17821 atgtgaaatc gtgccatcaa gataaagcag ctgctcaacg ttcagtttcg ctttggcata
    17881 acaggcaaaa tcataaaaat ttgtcgcctg ctggctcaac aaaaacacgg cattcccatg
    17941 tttattaatc ccaacaccgt tacgaatttt gcgtgaggcg acgttgggat gaatacgcgg
    18001 attaattaca ccgttttcca tcaacattgg ccctgactgc accgcaaact gaatctcttt
    18061 actggttttg aaggcatcca gacgaacgat gccgacttta tctcccgcga cataaaacac
    18121 gccgccagga cggataaaga aattcccttc acctgaagcg agatttaacg ccaccttctg
    18181 ctgaccgttt tcgatgtaca aaccgagcgg cgcatagctt tcatcataga tgccgccgtt
    18241 catcgccatc tgcacctgac cctgactatt catatccgcc agcagagcat gtaacgttcc
    18301 ccacgcttcg ccattagctt tttgccagta cattttcacc cgctctgttt gaggattaac
    18361 ggtatacgcc tgtacggtca gcgtcggatc tgagagtgcg caatcatcag cggcaacagc
    18421 aaacaaggga agtaaggtga gggtgagaaa aatccgtttg agattcaagg tgatcattcc
    18481 tttaccaatg agtagccgat gcgccattat aggtcctgga tgttggattt ttttatcctg
    18541 ttagcgacct tgacgagtac caaaaagcgc gaagttcaac tattgttctg tggtgttctg
    18601 ttgcgtgttg acggcaaaat tttgctggcg taacatgcgc gcacgatcac tctaagagga
    18661 cattcgcctt ggacacaccc agtagatact ggctcactat cctgtcatcc aggatcaact
    18721 cctaaggcta tccctttttg ctgatagcct tagcggttgt cagcgacctc aatttttccc
    18781 gtcgcgctga gtcaggctgt ttaatggtct gaaacccaat ttgtttctgt gtgcccaccg
    18841 aactgtccga tattttaagc attgggagtc ccggtcatgc tgagcgcatt tcaactggaa
    18901 aataaccgac tgacccggct ggaagtcgaa gagtcacaac cccttgtaaa tgcagtatgg
    18961 attgatcttg tcgaaccgga cgacgacgag cgactgcgcg tacaatctga acttggccag
    19021 agcctggcaa cccgcccgga actggaagac atcgaagcat cggcacgttt ctttgaagac
    19081 gacgacggcc tgcatattca ctccttcttc ttctttgaag atgcggaaga tcacgccggt
    19141 aactccactg tggcatttac catccgtgat ggtcgtctgt ttactctgcg tgagcgtgaa
    19201 ctgcccgctt ttcgtctgta tcgtatgcgt gcccgtagcc agtcgatggt agacggtaac
    19261 gcctacgagt tgctgctgga tctgttcgaa accaaaatcg aacagttggc agatgaaatt
    19321 gaaaatatct atagcgacct ggagcagttg agccgggtga ttatggaagg gcatcagggc
    19381 gatgagtacg acgaggcgct ctccacgctg gcggaactgg aagatatcgg ctggaaagtt
    19441 cgcctgtgtc tgatggatac ccagcgcgcg ctcaacttcc tggtacgcaa ggcgcgttta
    19501 ccgggtgggc aactggagca ggcgcgtgaa atcctgcgag atatcgaatc cctgctgccg
    19561 cataacgaat ccctgttcca gaaggtgaac ttcctgatgc aggcggcgat gggctttatc
    19621 aacatcgagc agaaccgcat catcaaaatc ttctcggtgg tatccgtggt gttcctgcca
    19681 ccgacgctgg tggcttccag ctacgggatg aactttgaat ttatgccaga gttgaagtgg
    19741 agtttcggct acccaggcgc gattatcttt atgatcctcg cgggcctggc accgtatctg
    19801 tactttaagc ggaagaactg gttgtaatat gagtgccgga taaagctatt tttttatccg
    19861 gcttatttct aaaaattgtc ttttttaaag tcatgcggat tgaaaactcg ctctatttta
    19921 ttttcttgtg ttaattgata ctcagtaaaa tcgttaatac caatatgagt gttacgataa
    19981 gggtattcat taataaccat taagtaattt ttcacgatgc aattatcatt gctcttaatt
    20041 gtcgggaaac agtagagatc ttcatacggc ccttcgatat attttttata gtcagtgaag
    20101 ccattaccga tatcaatgac ataccaggta tgccaactac cttctggtag gggatgttga
    20161 tttttaattt cattctgatg cttcagatac caatttattt tatcgctgtc agtccatggc
    20221 agatgatcaa cgaccagaaa gacagaattg ccagcatcag agtatgtata caaaatctgt
    20281 acaggacgca atgtccataa attatagatg cagcatagaa gaaataaaca taatataatt
    20341 ttctggagtt tgcttttcat agataatttt cgattctttc gatagtgtta aaattggtga
    20401 aaaaaggttt gaaagcaaag tctttatgcc gttgtaaaaa gaaccatatg cgaaaaaatc
    20461 tatatttgct gtaagtcttg tttttgatat cagttacatc aagaccgaag tggtcctgtg
    20521 ctacaaaatg aattgttgca ctccatccta tagcgtagtt ttgaaaactt aaaagagata
    20581 ttttttgag
//
LOCUS       DAZGXZ010000092        20371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000092 DAZGXZ010000000
VERSION     DAZGXZ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.092"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(149..1252)
                     /gene="gldA"
                     /locus_tag="ACHKJF_003465"
     CDS             complement(149..1252)
                     /gene="gldA"
                     /locus_tag="ACHKJF_003465"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP0950767.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1263..1925)
                     /gene="fsa"
                     /locus_tag="ACHKJF_003466"
     CDS             complement(1263..1925)
                     /gene="fsa"
                     /locus_tag="ACHKJF_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0950768.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1937..4438)
                     /gene="ptsP"
                     /locus_tag="ACHKJF_003467"
     CDS             complement(1937..4438)
                     /gene="ptsP"
                     /locus_tag="ACHKJF_003467"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0950769.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFSRSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRTVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            4747..5826
                     /locus_tag="ACHKJF_003468"
     CDS             4747..5826
                     /locus_tag="ACHKJF_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP0950770.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGFSTDEKEDDLDLDFEIN"
     gene            5841..6161
                     /locus_tag="ACHKJF_003469"
     CDS             5841..6161
                     /locus_tag="ACHKJF_003469"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0950771.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6212..8509
                     /locus_tag="ACHKJF_003470"
     CDS             6212..8509
                     /locus_tag="ACHKJF_003470"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0950772.1"
                     /translation="MTNRISRLKTALFSNTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8475..9353
                     /locus_tag="ACHKJF_003471"
     CDS             8475..9353
                     /locus_tag="ACHKJF_003471"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP0950773.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9355..9696
                     /locus_tag="ACHKJF_003472"
     CDS             9355..9696
                     /locus_tag="ACHKJF_003472"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0950774.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9683..10534)
                     /locus_tag="ACHKJF_003473"
     CDS             complement(9683..10534)
                     /locus_tag="ACHKJF_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0950775.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10749..12482)
                     /locus_tag="ACHKJF_003474"
     CDS             complement(10749..12482)
                     /locus_tag="ACHKJF_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0950776.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12665..15316)
                     /gene="ppc"
                     /locus_tag="ACHKJF_003475"
     CDS             complement(12665..15316)
                     /gene="ppc"
                     /locus_tag="ACHKJF_003475"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP0950777.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15668..16819)
                     /gene="argE"
                     /locus_tag="ACHKJF_003476"
     CDS             complement(15668..16819)
                     /gene="argE"
                     /locus_tag="ACHKJF_003476"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP0950778.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            16973..17977
                     /gene="argC"
                     /locus_tag="ACHKJF_003477"
     CDS             16973..17977
                     /gene="argC"
                     /locus_tag="ACHKJF_003477"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP0950779.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17985..18761
                     /gene="argB"
                     /locus_tag="ACHKJF_003478"
     CDS             17985..18761
                     /gene="argB"
                     /locus_tag="ACHKJF_003478"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP0950780.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18822..20195
                     /gene="argH"
                     /locus_tag="ACHKJF_003479"
     CDS             18822..20195
                     /gene="argH"
                     /locus_tag="ACHKJF_003479"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP0950781.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         4615 a         5209 c         5697 g         4850 t
ORIGIN      
        1 cccaaggccc gatacggcat gcggactaca ggcagagaaa actggccgat tttctctgca
       61 ctggagggat aatacaaggc ctgataagcg tagcgcatca ggcaattttg cgttcaaact
      121 cccggacaag ccgggagttt ggagtaggtt attcccactc ttgcaggaaa cgctgaccgt
      181 actggtcggc taccagcaga gcggcgtaaa cctgatctgg cgtcgcgccg ccaggcatgt
      241 tgtggatggt ttcaccttct gcacatgccg cttcagccac aattcgcatt ttcgccggga
      301 catcttcttt aatatccagt tgagcgagag ttattggcaa acctaccgca tggctaagtg
      361 cagctacggt ttcgatttcc tcaaccggtg cgttttccag aaccagctgc gtcagcgtac
      421 caaatgccac tttttcaccg tgataatagt gatgcgcgtc cgggatagcg gtcaggccgt
      481 tatgcaccgc gtgagccgcc gccagcccgc cgctttcaaa accaacgccg ctcaaatagg
      541 tgttcgcttc aatcacgcgc tccagcgccg gggttactac atgctgttcg gcggcaagca
      601 tcgctttttc gccttcttcc agcagggtgt tgtagcacag ttcagccagt gccagcgccg
      661 cctgggtgca cttgccgccc gccatggtgg tcgcgccgct acgagagcaa gcacgcgctt
      721 caaaccaggt tgccagcgca tcgccgatac ccgccgctaa tagacgtgca ggtgcgccag
      781 cgacgatttt ggtgtcgaca atgaccatat tcgggttatt tggcaacagc agatagcggt
      841 caaactcacc ctcatcggtg tagataacag acaatgcgct gcacggtgca tcggtagagg
      901 cgatagtcgg tgcgatcgct accggaacac ccatgaaatg tgccagtgct ttggctgtat
      961 cgagggtttt tccgccaccg ataccgagaa ttgcgccaca ctgcgcagtc tccgcgatgc
     1021 cacgcagacg gtcgatctca ttttgcgaac attcaccgcc aaacggcgca atttctacta
     1081 ccagtccagc atctttaaag cttttctcga cagtggattg agcaaaacct aaaacaaatt
     1141 tgtcacccac cactaaccag cgttctgcca gcggcttcag gtattcgccc agacgattaa
     1201 tcacatcagc gccctggatg tatttacccg gtgattgaat aatgcggtcc ataattgctc
     1261 ctttagagat gagtagtgcc aaatgcggca ttccagtcgt gttcgaactt ctctatagct
     1321 gactctaccg caggggtgtt gagcatttgt tgcgctacat ctaagggcag ggtgatggat
     1381 tcacatcctg ccagtaaaca gtccagcgcc tgacgcggcg ttttaaagct ggccgccagc
     1441 accatgcttt ctggtgcgtg catttctaac agcgcttgca gctcctgaac cgtacgaatg
     1501 ccgtccccgc cctgggcatc tacgcggtta acatacggag caacgtattt tgcccctgcc
     1561 agcgcggcca gtaacccttg tgcagcactg tacactgcgg tgccaagcgt agtaataccc
     1621 tcttttttca gcatcttaat ggcggccaga ccttcggaag tcaccgggat tttcaccaca
     1681 atgcccggaa tggcgtcgcg caggcgcttc gcttcttcca ccataccctg cgcgtcacga
     1741 ctcatagtct gagcaaacag aatgccctca tcaccaatcg ctttttgcag acgcggcaga
     1801 acttcccata tggactcctt gctggcagcg ataatgctcg ggttagttgt caccccggca
     1861 atggggaata tgcgtgccag acgttcgact tctgcgacgt tagcggtgtc cagatacagt
     1921 tccatgatgt ttccttttac agttccagtt catgttgcag caggctggcg atagcgtcgg
     1981 cagaggcggc gttaaccagc gcgttacgga attcttcgtg catgatgcgg cgagcgaggc
     2041 gcgagaaaat gcgcatgtgc tgatcgcccg cagcgtgttt gttcagggtt aacataatga
     2101 tgaattgcgc ttcatcatcg ccccagcgca ccggcgcttg cagacgcgcc acgctgatgg
     2161 tggattgctc aatgtgttct gatttgctgt gtggaatggc aaaactaaag cccagaccgg
     2221 tagagaaaac ggcctcgcgc gcccataagt cggcttccag tttacgcgga tagcggcagc
     2281 ggcccgccag cagcaggtta tcggtcatgc ctttgagcac ttcttctttg ctgcgccagt
     2341 cactttccag cgtgatgcac tcggcggtga ccagcggtgc gtcctgttgg gtcatgcgga
     2401 attgcgccag caggtgttcc acttccagcg aagtacggca ggccattgcc tggttgagca
     2461 acttgcggca ctcacggcta tcaagttgcg ccatccgcgc tttcgccgcc ggaattgatg
     2521 gtgcgctcat gctgagttca tccagcccta agccgaccag caacggcagc acggaacctt
     2581 tcgctcccag ctcaccgcac agaccaatcc atttaccctg gcgatgcacc gcctgcacgg
     2641 cgtaatcgag cgcccgcaag aatgccggat tcaggctgtt gtagtgacga gtaaccttag
     2701 cgttatcgcg atccactgcc agcagatatt gcgtcaggtc attactacca atactaaaga
     2761 aatcaatctc ttcgcagcat tgatcgatga tgaacatcac cgatggcact tccagcataa
     2821 tgccgagctg gatcttctca tcaaacggaa tgtgttcgtt acgcagttgc tgtttggctt
     2881 ccgccagttt ttctttcacc cataagatct cttccattga agagatcatc gggatcatga
     2941 ttttcaggct gccgtgagcg gaggcgcgca ggatcgaccg tagttgtgtg gtgaacagcg
     3001 aggcgtattc ttcataaata cgcacagtgc gataaccgag gaacgggttt gcctctgcgg
     3061 gaatgttcag ataatcaacg ggtttgtcgc cgccaatgtc catagtgcgc acaataatgc
     3121 tgcgtccgtt ggcagactcc agcgcctgac aaaaaatgtt gtacagctcg ctttcgcccg
     3181 gtgcactggt gcgatccata tagagcattt cagtgcggaa caaaccaacg ccttccgcac
     3241 cattgccgaa tgcggcctgc gcttccacgg agtgagcgat gttagcggca atttcaatgc
     3301 ggataccgtc agcggtacgg gcttgttggg tcagccagac acgctgttgc tcacgcaggg
     3361 cgtcctgtac gcgggcttct tgctgataat aacgagctac ggcttctcct ggctcaacca
     3421 caatcgcccc agcgttgccg tcgatataaa tcgtttgatg ctgccacgga gtgagggcat
     3481 caatatccac accaaccagc gtcggaatgt tgaacgaacg ggcaaggatc accgtatgtg
     3541 aggtggtgcc gccgcttttc agtaacaatc ctttgaggtg atttttatcc agttcgagga
     3601 actggctggg ggtcagttca tcagccatac aaatggcgga ctgcgtcagt ttgcccggtg
     3661 ccgggaagcg ttgctcacca tagatttgct ggagtaactg gaagcagaca tcgcgtacgt
     3721 ccagggcacg ttcttgcagg tagctgctgc tggaacggga aaactcttca caaaagtgat
     3781 tcgcactggc gacaatcgct tcggcgcagc ttaatccggc gctgacgcct gccagtaaat
     3841 gttcgcgcag ggaagtatcg ccagccaggg atcggtgcgc ttccagaatc gcgctggtgg
     3901 caccgtcgct atccagcaga cgaaactcaa tgtttttcag taccagcgtc aggccgtttt
     3961 ccagtgcgga ttgctcggcg tcaacgcctt tggctgcggg aagattaccc agcgcattga
     4021 gatctaaaga agagatcggc gtcagaatgc cgcctgcact accgctgcac acggtgcggg
     4081 cgcggataat ttgcggattc agattggtca gtgaaaccgg cagtggttcc agttcgtcag
     4141 atttaacttc cgccagcggc gcatcgcagt gggggaattc atcgcgcagc cattggctta
     4201 aacgctggtg cgcttcctgt tcgtcggccc cggaaatcag taactggcag ttatcgcccg
     4261 ccagcgtatc ggtgccaatc agcgcaaggg cgcttttggc gttgcccttg cggtcagtgc
     4321 gcaggttatg ccactcaatt tgtgatgaaa aagtattaca cagcgtctca acgtggcttg
     4381 ccggacgcgc atgtacgccg ttaggtagct cacaaataaa ttccacaatc agggccattg
     4441 cctctctccc ggtacggttt tctgtttaca ggataagagc gcacggcaac ggcctgccat
     4501 gtgacaaatc tgccaaaagc tggacaaatg taatgtaacc gtcaatttgc gacgcgtctc
     4561 acaagacgct gttttgcggc atgcttccgg tttatcgcaa gttatgaggc ggatcgcatt
     4621 tttgtactga tattacaaaa gtccagtaaa tggccttttt atccactgtt tgctccgcct
     4681 gcgattgcct attgtttcct ccatcaaaaa tatggacatg ggctttcgca agcccgggag
     4741 cagcttatga atgagttggt gcagatcctg aaaaataccc gtcagcattt aatgacgggc
     4801 gtttcacaca tgattccctt cgtggtatcg ggcggtattt tgctggcggt ttccgtcatg
     4861 ttgtatggca aaggcgcagt gccggatgcc gtagccgatc cgaatctgaa aaaactgttt
     4921 gatatcggcg ttgcaggttt gacgctgatg gtgcctttcc tcgccgctta catcggttac
     4981 tccattgcag agcgttctgc gctggccccg tgcgctatcg gtgcctgggt tggtaacagc
     5041 tttggtgcgg gcttctttgg tgcgctgatc gccgggatta tcggcggcat cgtggtgcat
     5101 tacctgaaga aaattccggt gcataaagtt ctgcgctcgg tgatgcccat cttcattatt
     5161 cctatcatcg gcacactgat taccgcaggc atcatgatgt ggggcttggg cgagcctgta
     5221 ggggcgttga ccaacagcct gactcagtgg cttcagggga tgcagcaggg cagcattgtt
     5281 atgctggcgg tgatcatggg gctgatgctg gcgttcgata tgggcggtcc ggttaacaaa
     5341 gtggcctatg ccttcatgct gatttgcgtt gctcagggtg tttataccgt ggtggccatt
     5401 gctgccgtgg gtatttgtat cccgccgctg gggatggggc tggcgacgct gattggtcgt
     5461 aaaaatttct ccgcagaaga gcgcgaaacc ggcaaagcgg cgctggtgat ggggtgcgtt
     5521 ggggttactg aaggggcgat tcctttcgcc gctgccgatc cgctgcgtgt cattccttcc
     5581 atcatggtcg gttctgtttg tggtgcggta actgcggcgc tggtcggtgc gcagtgctat
     5641 gcaggctggg gtggtctgat tgtgctgccg gtggttgaag gcaagctggg ttatatcgca
     5701 gcagtggctg tcggagcagt ggtgacggct gtttgcgtga acgtgctgaa aagtctggcg
     5761 cgtaaaaatg ggttttcgac tgatgagaag gaagacgacc tggatttgga ttttgaaatt
     5821 aattaattga ggaacgagcc atgacgaaaa ttattgcagt aaccgcgtgt ccatctggtg
     5881 ttgcccatac ctatatggcg gcagaggcgc tggaaagcgc cgcgaaagcg aaaggctggg
     5941 aagtgaaagt agaaactcag gggtcaattg gtctggaaaa cgaactgact gcggaagacg
     6001 tggcgagcgc cgatatggtg attctgacca aagatatcgg catcaagttt gaagagcgtt
     6061 ttgctggcaa gaccatcgtg cgcgtcaaca tcagcgatgc ggtaaaacgt gctgacgcca
     6121 ttatgagcaa aattgaagct cacctggcgc aaaccgctta agttcttctc ccgctcgcaa
     6181 gggcgggttc gctttcccac aggagttcct catgacgaat cgtatctctc gcctcaaaac
     6241 tgcactgttt tccaataccc gtgaaatctc gctggagcgg gcgctgcttt ataccgccag
     6301 ccatcggcaa accgaaggcg aaccggtgat attgcgccgg gcgaaagcaa cagcgtatat
     6361 ccttgaacat gttgaaattt cgattcgtga tgaagaactg attgccggta accgcaccgt
     6421 aaaaccgcgc gccgggatta tgtcgccgga aatggaccct tactggctgc tgaaagagct
     6481 ggatcaattc ccgacgcgtc cgcaggaccg ctttgctatc agcgaagaag ataaacgtat
     6541 ctaccgcgaa gagttgttcc cgtactggga aaaacgttcg atgaaagatt tcatcaacgg
     6601 gcagatgaca gatgaagtaa aagccgcgac cagcacgcag attttcagca ttaaccagac
     6661 ggataaaggc caggggcaca ttattattga ttacccacgc ctgctgaatc acgggctggg
     6721 ggagctggtg gcgcagatgc agcaacattg tcagcaacag ccggagaatc acttttatca
     6781 ggccgcgctg ttactgctgg aagcctcgca gaaacacatt ctgcgttacg ctgaactggc
     6841 ggaaacaatg gcggcaaact gcacagatgc ccagcgtcgc gaagaactgc tgactattgc
     6901 ggagatctcc cgccataacg ccgaacataa gccgcagacg ttctggcagg cgtgccagtt
     6961 attctggtac atgaacatca ttctgcaata cgaatccaac gccagttcgc tgtctttagg
     7021 gcgcttcgac cagtatatgt tgccgttcta tcaggcttct ttaacccagg gcgaagatcc
     7081 ggcgttcctg aaagaactgc tcgaatcttt atgggtgaaa tgcaacgaca tcgtgctgtt
     7141 gcgctccacc agcagcgcgc gttatttcgc aggtttcccg accggctata ccgcactgct
     7201 cggcgggtta accgagaacg gacgtagcgc ggtgaacgtg ctttcgttcc tttgccttga
     7261 cgcctatcaa agcgtgcaat taccgcaacc gaacctcggc gtgcgcacta acgccttgat
     7321 cgacacgccg ttcctgatga aaaccgccga aaccattcgc ctcggcaccg gtattccgca
     7381 aatctttaac gatgaagtgg tggtaccagc gttcctcaac cgtggcgttt cgctggaaga
     7441 tgcgcgcgac tattccgtag tgggctgtgt ggaattatct attcccggca gaacctacgg
     7501 cttgcatgac atcgcgatgt ttaatctgct gaaagtgatg gaaatctgcc tgcatgaaaa
     7561 tgaaggcaat gctgcgctga cttatgaagg tttactggag cagatccgcg ccaagatcag
     7621 ccactacatc accctgatgg ttgagggcag caatatttgt gatatcggcc atcgcgactg
     7681 ggcacctgta ccgctgctct cgtcttttat cagcgattgt ctggaaaaag gtcgcgatat
     7741 taccgatggc ggcgcgcgtt ataacttctc cggcgtacag gggatcggta tcgccaacct
     7801 gagcgattct ctccatgcgt tgaaagggat ggtttttgag caacagcgtt taagttttga
     7861 cgaattgctg tcggtattaa aagccaactt tgcaacgcca gaaggcgaaa aagtccgtgc
     7921 tcgcttaatt aaccgcttcg agaaatacgg taacgatatc gacgaggtgg ataacatcag
     7981 cgccgaactg ttacgccact actgcaaaga agtggaaaaa taccagaacc cgcgcggtgg
     8041 ttacttcacg ccgggatcgt ataccgtttc tgctcacgtc ccgttgggat cggtggttgg
     8101 cgcgacgcca gacggtcgtt ttgccgggga acaactggca gacggcggct tgtcacctat
     8161 gctgggccag gacgcacaag ggccaacagc ggtactgaag tcagtcagta agctcgataa
     8221 tacgctgctg tctaacggta cgttgctgaa cgtgaaattc actccggcga ccctggaagg
     8281 tgaagcggga ttacgcaaac tggccgactt cttacgggcg tttacccagc ttaagttgca
     8341 gcatattcag tttaacgtgg tgaacgccga cacgttgcgg gaagcgcaac agcgcccaca
     8401 agattatgcc gggctggtgg tgcgcgttgc cggatacagc gccttctttg tcgaactgtc
     8461 gaaggagatc caggatgaca tcatccgccg gacagcgcat cagctgtaac gttgtggaaa
     8521 cgcgccgcaa tgatgtggcg cgcattttca acattcagcg ttattcactg aatgacggcg
     8581 agggcattcg tacggtggtc ttttttaaag gctgtccgca tctttgccca tggtgtgcta
     8641 atccggagtc gatctccggc aaaatccaga cggtacgcag agaggcgaaa tgtctgcact
     8701 gtgcgaaatg tttgcgtgat gcggatgaat gcccctccgg ggcgtttgaa cggattggtc
     8761 gcgatatcag ccttgacgcg ctggaacggg aagtgatgaa agatgacatt ttctttcgca
     8821 cgtccggcgg cggcgtcacg ctttctggcg gcgaagtgtt aatgcaggcg gagtttgcta
     8881 cccgtttttt acagcggctg cggttgtggg gcgtgtcatg cgccattgaa actgccggag
     8941 acgcgccagc cagcaagctg ttaccactgg cgaaattgtg cgatgaagtg ctgttcgatt
     9001 taaaaattat ggacgcgact caggcgcggg atgtggtgaa gatgaacctg ccacgcgtgc
     9061 tggagaatct gcgtttgctg gtgagtgagg gtgtcaacgt gatcccgcgt ttaccgttga
     9121 tccctggttt cacgctcagc cgggagaata tgcagcaggc gctggatgta ctgatcccgc
     9181 tgaaaatcag gcagatccat ctgttaccgt ttcatcagta cggcgaaccg aaataccgcc
     9241 tgctggggaa aacatggtcg atgaaagagg tgcctgcgcc gtcgtcagcc gatgtgacaa
     9301 cgatgcgcga aatggcagaa cgggccggat ttcaggttac cgtgggaggt taaaatggca
     9361 tacctggtgg cagtaaccgc ctgcgtaagc ggcgtggcgc atacttatat ggcggcggaa
     9421 cggctggaaa agttgtgcca gttagagaag tggggcgtca gcattgaaac tcagggcgcg
     9481 ctgggaacgg agaatcgttt agcggacgag gatatccgtc gggcggatgt tgctctgttg
     9541 attacggata tcgagcttgc cggtgccgag cgatttgaac attgccgcta tgtgcaatgc
     9601 agcatctacg cattcctgcg tgagccgcag cgggtaatga gcgcggtgcg caaagtgctt
     9661 tctgcgccgc agcaaaccca tcttattctg gagtagtcgg tttttctgtc agttggctgt
     9721 ggtactgccg acgatattcc gacggcgagc gttcggtgtt tttacgaaac agacggcaga
     9781 agtagttgct gtcgacaaag ccgcaggcgt gcgccacttc ttttaccttc aggtcatagc
     9841 ctttcaataa cgtcttagcg tgctccagtc gcgtgtgatt caggtattcg ttaaaaccaa
     9901 tggcccccgt tttttgaaac aggtgcgaga ggtaatttgg cgaaatatag aatgcctgtg
     9961 cgacagattc gcgggtaagc gcggaggcat agcgttcgtc gatataatcg cgaatagctt
    10021 cgaatagagc ctggctgcgc gaggcggtct ggatttggct gccgagcaaa tcgcggcagt
    10081 ggctaagcag gctggcgacg ataagccttg ccgtttgctg ctcctgcggc tgcatttgca
    10141 tttcattgag cgtttgtagc agaaaagaac ctatgcgtgg gccgcgccgg gcgacgtgtt
    10201 gcttcgccag attttgatat tgtttgccat cccattgcac gacgctgaag ccgagttgct
    10261 gtttgccaaa cagcacgcta aaggtagtag cgggggcttg ccattgtgga aaattccagc
    10321 caccagcggg aacgtacagc acatcgccgg gaactaacgt tgctccagcg ccggtatcaa
    10381 caaactcacc ttccagcact atttccagcc gtggaaaatc gacctgatac gcaagatcgg
    10441 gcgccggacc gttactgctg gcaaagtaaa tctggcgcag ggacagcggg ccgttgatca
    10501 ggcgggagag cagatagctg acgtcgtgat acatgttatt cccttaggta tgtagacggg
    10561 aaaacagtaa ctggcattat taaaaaaggc cacctgaaaa gtgtggcctg aggggagttc
    10621 gatgtaaaca ttgcggcagt tgtattgccg gatgtggcgt aaacgcctta ttcagtctac
    10681 cgccgcgatc tgtaggccgg ataagacgcg tcaagcgtcg catccggcaa atagcgcctg
    10741 gctgataatt actgattacc tacctggtcg ccatacggca gagtgtcgta atcgatcagc
    10801 gcgtttttct tgtacgggtt accaatccag cgggtagttt ctttgaactg cggattcacc
    10861 actgaacggg ttggatcgta accgtcgtaa gataagcccg ccaaatctga ccaggtgtgg
    10921 atcagttccg ccaggctgta tttacgatcg acatcctgtg agaaatcacg gggatgagtc
    10981 gcttgccatt tttccgacgt ccacagcagg aacggaatgg tgtacatgtg gcgcgtcggg
    11041 ttgtcttcgt tacgcccctg agttttgtgc ggcggcgtgt cgtaaacctc ttcaccgtgg
    11101 tcagagaagt aaaccaggaa tccgttcggg tctgccgctt taaagtcttt aatcaggctg
    11161 gcaaccacat gatcgttata caggttagcg ttgtcataat cgttatatga ttccagctct
    11221 tctgcgctta atcctggcgg aacatgatcg gtattgccat caaacttgcc ctgattttcc
    11281 gggtagcggt atttgtattt gatatgcgta cccagcagat gaacgatgat cagtttcttc
    11341 ggcgcagggt cattcagcac atcctggaac ggcttcagca cgttggtgtc gtattcacgc
    11401 gcactctgcg tacgttgctg gttcatgtag tactgcttgt cggtctggcg cgaaaatacc
    11461 gtcagcatgg tattgcgggc ggtcatcgtc tgctggttgg tgatccagaa ggttttataa
    11521 cccgcctgtt tcatcatgtt catcagcgac ggctgcgtca gatacagatc cgggttcttt
    11581 tcattggcaa aggtcagcgc ctgttgcagg atttcaatgg tgtacggacg agacgtaact
    11641 acgttattaa acacggtcag attcggatcg gttttatgca gcgcatccag ctccggcgtg
    11701 gtttcacgcg gataaccgta cagactcatg cgtccgcgct gggtcgactc gccaatcacc
    11761 agcactaaag tgcgcggttc gttacccgat tcatctttga aattagccag tggcggcaag
    11821 gcattatttt cattcagtaa ctttgttagc gagtttagtt gctgacgata ctgataatag
    11881 ccggtcagga attgccacgg tgcggcaggc tccatacgcg aggccaggtt atccaacgtt
    11941 ttctcaaacg gcttgttttt gataaacgta ttcatggcga tcggatgcag aatcaagcca
    12001 taaagcaggg caaaagagac aacataacgc cacggctttg gaatatagac cgggcgcagg
    12061 cgtgtccaca gcagaactgc caccgccgta taggccagcg cgataagcac aattttcagg
    12121 ctgaaatact ggcttaaata ctcgctggct tcgttggtgt tggtttcgaa catcacaaac
    12181 agaacgctct gcgagaactc ctgcccgtag atgacgtagt agcatagcgc cgccagagag
    12241 gccgcccata gcaccacgcc gattactgcg gcaataattt taatccgctt cggaaagagg
    12301 aataccggga tcaaccacag cgagctgaat aacagcgagt cgcgaatgcc gttggtgcca
    12361 ctataaccac tgatgtaaat aatggcctgt agcagagtag agaaaaacca aaagtagagc
    12421 agtgcccaac ccagggcttt ccagctaaaa aggggtttag cctggacttc tgtggaatgc
    12481 atagtaagaa cctgtcttga aaaaaatatc gccgaatgta acgacaattc cttaaggata
    12541 tctgaaggta tattcagaat ttgaataaaa tgcagacaga aatatattga aaacgagggt
    12601 gttagaacag aagtatttca gaaaaccctc gcgcaaaagc acgagggttt gcagaagagg
    12661 aagattagcc ggtattacgc atacctgccg caatcccggc aatagtgacc attaacgctt
    12721 gttcgacgcg aggatccggt tcctggcctt ctttttctgc ctggcgggag cggtgcagca
    12781 actcggcctg caatacgttc agcgggtcgg tgtaaatatt ccgtagctga atagactctg
    12841 caatccacgg cagatcggcc atcagatggg aatcgttggc aatcgccagc accactttga
    12901 tgtcttcttc ttgcaggttg cgtaactctt tacctaacgg ccacagtgct ttgtctacca
    12961 gacgttgatc atagtattcc gccagccaca ggtctgcttt ggcgaagacc atctccagca
    13021 tgccgagacg cgtcgagaag aatggccaat cgcggcacat ggcttccagc tcgctctgtt
    13081 tgccatcttc gaccactttt tgcagcgccg tacctgcacc cagccaggcg gggagcatca
    13141 ggcggttttg cgtccaggca aaaatccacg gtatggcgcg cagcgactcg acgccgccgg
    13201 ttgggcgacg tttcgccgga cgtgaaccca acggcagttt gcccaattct tgttccggcg
    13261 tagcggagcg gaagtaaggc acaaaatctt tgttttcacg tacgtagcca cggtagagat
    13321 cgcaggagat gactgacagt tcatccataa tgcgacgcca gctctctttc ggctcaggtg
    13381 gtggcagcag gttggcttcc agaatcgccc cggtgtacag cgacaggctg ctgacggtga
    13441 tttctggcag accatattta aagcggatca tctcgccctg ttcagtcacg cgcaggccac
    13501 ctttcaggct tcctggcggt tgtgacagca gcgccgcatg agcaggtgcg ccgccgcgac
    13561 caatggaacc gccgcgaccg tggaacaacg tcagctcaat acccgctttt tcgcaggttt
    13621 tgattaatgc atcctgtgcc tgatattgcg cccaggaagc tgccatcact cccgcatctt
    13681 ttgctgagtc ggaatagcca atcatcacca tctgtttgcc ctgaatcagg ccacgatacc
    13741 agtcaatatt gagcagctgg gtcatgacat cgttggcgtt gttcaggtca tcgagggttt
    13801 caaacagcgg agcaaccggc attgcaaacc cgatacccgc ttctttcagc agcaggtgga
    13861 cagccagcac gtcggacggc gttttcgcca tcgagatcac gtaggcggca atggagcctt
    13921 gcggcgcttc ggcaatcacc tggcaggtat cgagcacttc gcgcgtttcg gcgcttggtt
    13981 gccagttgcg cggcagcagc ggacgtttag agttcagttc gcggatcagg aacgcctgtt
    14041 tgtcggcctc tgaccagctt tcgtagtcgc cgataccgag gtagcgggtc agctcgccca
    14101 gcgcttcggt atggcgggtg ctctcctgac ggatatcaat acggaccagc ggtacgccga
    14161 aacatttcac gcggcgcagg gtgtcgagca gatcgccgtt ggcgataata cccatgccac
    14221 acgcctgaag tgactggtag caagcgtaga gcggttccca cagttcttcg ttttgtgtca
    14281 gcagcccttc tggttttggc agttcttcgc ctttcaggcg cgcttccagc catgcctgtg
    14341 tcgccatcag gcgagaacgc aggtttttca tcagatagcg atacggttct gcggcacctt
    14401 cttcgccaac cagcgccagc agttcagggg tcgcttcaac catcgacagt tcagaaacca
    14461 gaacctgaat atctttcagg aacagatcgg tggctttcca gcgactgagc agcaggacgt
    14521 ggcgggtgat atcggcagtg acgttcgggt tgccgtcgcg gtcaccgccc atccacgagg
    14581 taaaacggac cggaacaaat tcgacgggca gtttgtagcc gagattctct tccagttgtt
    14641 cattcagttc gcgcaggtaa ttcggtacgc cttgccacag gctgttttct actacggcaa
    14701 agccccattt ggcttcatct accgggcttg gacgcagctt acggatttca tcggtatgcc
    14761 atgactgggc gatcaactgg cgcaggcgac gcatcagctg gttgtgttcg tagtcagcga
    14821 tatctttgtt atcgagctgt tttaagcagg cgttcacttc caccattttg tggatcagtg
    14881 tacgacgggt aatttcggtt gggtgagccg tgaggaccag ttccagcgac agcgattcca
    14941 ctgctttttt gatggtgtct tcgctcagtt ccggctggtt tttcagttta cgcagggtgc
    15001 gggcgatcac ttccgggttg ctggcagctt cgcctttcgg cgaaatgctg tggtattgct
    15061 cggcggtgtt ggccaggttc agaaactgac taaacgcacg cgcaacgggc agcagctcgt
    15121 cgttcgacaa attttgtaag gtggtgagca actcctggcg gttagcatca ttgccagcgc
    15181 gtgaagattt ggacaactta cggatagttt ctacgcgttc aagaatgtgt tctcccaacg
    15241 catccttgat ggtttctccc agcactttgc cgagcatact gacattacta cgcaatgcgg
    15301 aatattgttc gttcatatta ccccagacac cccatcttat cgtttgatag ccctgtatcc
    15361 ttcacgtcgc attggcgcga atatgctcgg gctttgcttt tcgtcgtctt ttataaagcc
    15421 acgtaaaagc ggtgacgtca aatgctgcga aatcgcttca gcaaacgaat aaatagcagg
    15481 aatttacgtc attaaattca cgacgcttta aataagcgta acttatggaa atgttaaaaa
    15541 atcgccccaa gtaacaccaa aggtgtaggt cggataagat gcgcaagtat cgcatccgac
    15601 attattgcgg cactggagtt tgacaacagt gccggatgcg gcgcgagcgc cttatccggc
    15661 ctacgtttta atgccagcaa aaatggtgaa ttacctgggt tatcagttcg cgggtgggct
    15721 tgataaaccg tgtttccaga tattcatcag gttgatgagc ctgattaatt gagccaggcc
    15781 ccaacaccag cgtcgggcat aacgtttgaa taaacggcgc ttcggtacag tagttcacca
    15841 cttcggtctt tgctccgagc aatttctcaa ctacttcaac cagttgatga ttcggtgggc
    15901 attcatagcc agggatcggc ggatgcagct cgtcgaccgt cagacgaccc ggccagcgtt
    15961 cgctcaccgg agccaatgca tcgttgagca aaccattaag ttcattgagt gtcatgccag
    16021 gcagcggacg aatatccata tgcaactcac agcaagcgca aatacggtta gaagcgtcgc
    16081 caccgtgaat atgcccgagg ttgagcgtag ggtatggcac ggtaaacgct tcgtagtgat
    16141 aacgttcttt caggttatcg cgcaattgca aaatatgccc gatggcgtcg tgcattagtt
    16201 cgatagcgtt aactccgcgt gctggatcgc tggagtgccc cgactggccc tgaatacgga
    16261 tggcgttaga gatatgacct ttatgtgcgc gtaccggttg tagtgacgtc ggttcgccaa
    16321 tgatggcgca atccgggcgc agggcggtag tttcggcaaa ataacgcgct ccggccatac
    16381 tggtttcttc atcagcagtc gccagaatgt agagcggttt tttcagtttc gtgacgtcga
    16441 catcgcgtag cgcatcaagg ataaacgcaa aaaagccttt catgtcggcg gtgcctaagc
    16501 cgtaaagctt gccgtcatgc tccgtcagtg taaacggatc gcgcgtccag cgaccgtcat
    16561 caaatggcac cgtatcggta tgccccgcca gcaacaagcc gccagccccc tgtccgcaac
    16621 tggccagcat attaaatttg ttgcgagttc ctggaacagg ctgcacttcc acattgaagc
    16681 ccaaatcttt aaaccagtcc gccagcagag tgattaaatc tgcattgctt tgatcgagtg
    16741 cctcttccgt ggcgcttatt gaaggtgtgg caatcagagc gcggtaaatc tcgataaatg
    16801 gcggtaattt gtttttcatt gttgacacac ctctggtcat gatagtatca atattcatgc
    16861 agtatttatg aataaaaata cactaacgtt gagcgtaata aaacccacca gccgtaaggt
    16921 gaatgtttta cgtttaacct ggcaaccaga cataagaagg tgaatagccc cgatgttgaa
    16981 tacgctgatt gtgggtgcca gcggctacgc tggcgcagag ctagtgacct atgtaaatcg
    17041 ccatccgcat atgaacataa ccgctttgac tgtttcagcg caaagcaatg atgcgggaaa
    17101 gttaatctcc gatttgcatc cgcagctaaa aggcatcgtt gatctgccgt tgcagccgat
    17161 gtcggatatc agcgagttta gcccaggggt ggacgtagtg tttctcgcca ccgcccatga
    17221 agttagccac gatttagcgc cgcagtttct tgaagcgggc tgcgtggtgt tcgacctttc
    17281 cggcgcgttt cgtgttaacg acgccacctt ctatgaaaaa tattacggct ttacccatca
    17341 atacccggaa ctgttggaac aggcagccta cggtctggcg gagtggtgcg gtaataaatt
    17401 aaaagaagcg aatttgattg cggtgccggg ctgttatccg acggcggcac agctggcgct
    17461 gaaaccgttg attgatgccg atcttcttga cctcaatcag tggccggtga tcaacgccac
    17521 cagcggcgtg agcggtgcag ggcgtaaagc ggccatttca aacagctttt gtgaagttag
    17581 cctgcaaccg tatggcgtct ttactcatcg ccatcaacca gagatcgcca cacacctcgg
    17641 tgctgacgtt atcttcaccc cacatctggg caatttcccg cgcggcattc tcgaaaccat
    17701 tacctgccgc ctgaaatcgg gtgtgaccca ggcgcaagtc gcgcaagtgt tacagcaggc
    17761 gtatgcccat aaaccgctgg tgcggctgta tgacaaaggc gttccggcgc tgaaaaatgt
    17821 cgttgggctg ccattttgcg atatcgggtt tgccgttcag ggcgagcatc tgattattgt
    17881 ggcgaccgaa gacaacttac tgaaaggcgc ggcggcacaa gcggtacagt gcgccaatat
    17941 tcgtttcggc tatgcggaaa cgcagtctct tatttaaggg tgcaatgatg aatccattaa
    18001 ttatcaaact gggcggcgta ctgctggata gtgaagaggc gctggaacgt ctgtttagcg
    18061 cactggtgaa ttatcgtgag tcacatcagc gtccgctggt gattgtgcac ggcggcggtt
    18121 gcgtggtgga tgagctgatg aaagggctga atctgccggt gaaaaagaaa aacggcctgc
    18181 gggtgacgcc tgctgatcag atagacatta tcaccggagc actggcggga acggcaaata
    18241 aaaccctgtt ggcatgggcg aagaaacatc agattgcggc cgtaggtttg tttctcggtg
    18301 acggcgacag cgtcaaagtg acccagcttg atgaagagtt aggtcatgtt ggactggcgc
    18361 agccaggttc gcctaagctt atcaactcct tgctggagaa cggttatctg ccggtggtca
    18421 gctccattgg cgtaacagac gaagggcaac tgatgaacgt caatgccgac caggcggcaa
    18481 cggcgctggc ggcaacgctg ggcgcggatc tgattttgct ctccgacgtc agcggcattc
    18541 tcgacggcaa agggcaacgc attgccgaaa tgaccgccgc gaaagcagaa caactgattg
    18601 agcagggcat tattactgac ggcatgatag tgaaagtgaa cgcggcgctg gatgcggccc
    18661 gcacgctggg ccgtccggta gatatcgcct cctggcgtca tgcggagcag cttccggcac
    18721 tgtttaacgg tatgccgatg ggtacgcgga ttttagctta agttttgttg gccggaggcg
    18781 cagctttccg gcattgaatt tcaaaataag gaaacagagt tatggcactt tggggcgggc
    18841 gttttaccca ggcagcagat caacggttca aacaattcaa cgactcactg cgctttgatt
    18901 accgtctggc ggagcaggat attgttggct ctgtggcctg gtccaaagcc ctggtcacgg
    18961 taggcgtgtt aaccgcagaa gagcaggcgc aactggaaga ggcgctgaac gtgctgctgg
    19021 aagatgttcg cgccaggcca caacaaatcc ttgaaagcga cgccgaagat atccatagct
    19081 gggtggaagg caaactgatc gacaaagtgg gccagttagg caaaaagctg cataccgggc
    19141 gtagtcgtaa cgatcaggta gcgactgacc tgaaactgtg gtgcaaagat accgttagcg
    19201 agttactgac ggctaaccga cagctgcaat cggcgctggt ggaaaccgca caaaacaatc
    19261 aggacgcggt catgccaggt tacactcacc tgcaacgcgc ccagccggtg acgttcgcgc
    19321 actggtgcct ggcctatgtt gagatgctgg cgcgtgatga aagccgtttg caggatgcgc
    19381 ttaagcgtct ggatgtcagc ccattaggct gtggcgcgct ggcgggaacg gcctatgaaa
    19441 tcgaccgtga acagttagca ggctggctgg gctttgcttc ggcgacccgt aacagtctgg
    19501 acagcgtttc tgaccgtgac catgtgttgg aactgctgtc ggctgccgct atcggcatgg
    19561 tgcatctgtc gcgttttgct gaagatctga ttttctttaa caccggcgaa gcggggtttg
    19621 tggagctttc tgaccgcgtg acttccggtt catcattaat gccgcagaag aaaaacccgg
    19681 atgcgctgga gctgattcgc ggtaaatgcg gtcgggtgca gggggcgtta accggcatga
    19741 tgatgacgct gaaaggtttg ccgctggctt acaacaaaga tatgcaggaa gacaaagaag
    19801 gtctgttcga cgcgctcgat acctggctgg actgcctgca tatggcggcg ctggtgctgg
    19861 acggcattca ggtgaaacgt ccacgttgcc aagaagcggc tcagcagggt tacgccaacg
    19921 ccaccgaact ggcggattat ctggtggcga aaggcgtacc gttccgcgag gcgcaccata
    19981 ttgttggtga agcggtggtg gaagccattc gtcagggcaa accgctggaa gatctgccgc
    20041 tcgacgagtt gcagaaattc agcccggtga ttgacgaaga tgtctatccg attctgtcgc
    20101 tgcaatcgtg cctcgacaag cgtgcggcaa aaggcggcgt ctcaccgcag caggtggcgc
    20161 aggcaattgc ttttgcgcag gcgcggttag agtaatttgt ttatattgct cccctgaaaa
    20221 tggggagctt accacgccta caaagtgcga cacttcgtag gcggataagg cgtttacgcc
    20281 acatcaggca gaaatttgtc agcattctgg tgagcttaaa tttaacgccc aaccagaacc
    20341 attatttgtc acattccttt ttatcattgt g
//
LOCUS       DAZGXZ010000093        19878 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000093 DAZGXZ010000000
VERSION     DAZGXZ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19878
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.093"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKJF_003480"
     CDS             complement(60..1652)
                     /gene="ybiT"
                     /locus_tag="ACHKJF_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP0950782.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKJF_003481"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="ACHKJF_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0950783.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKJF_003482"
     CDS             complement(2850..3968)
                     /gene="ybiR"
                     /locus_tag="ACHKJF_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP0950784.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKJF_003483"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="ACHKJF_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP0950785.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKJF_003484"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="ACHKJF_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP0950786.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKJF_003485"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="ACHKJF_003485"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0950787.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKJF_003486"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="ACHKJF_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP0950788.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="ACHKJF_003487"
     CDS             complement(7218..7283)
                     /locus_tag="ACHKJF_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950789.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKJF_003488"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="ACHKJF_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP0950790.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8704..9207
                     /gene="dps"
                     /locus_tag="ACHKJF_003489"
                     /gene_synonym="pexB"
     CDS             8704..9207
                     /gene="dps"
                     /locus_tag="ACHKJF_003489"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP0950791.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKJF_003490"
     CDS             9611..10357
                     /gene="glnH"
                     /locus_tag="ACHKJF_003490"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP0950792.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKJF_003491"
     CDS             10496..11155
                     /gene="glnP"
                     /locus_tag="ACHKJF_003491"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP0950793.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKJF_003492"
     CDS             11152..11874
                     /gene="glnQ"
                     /locus_tag="ACHKJF_003492"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP0950794.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKJF_003493"
     CDS             11991..14216
                     /gene="ybiO"
                     /locus_tag="ACHKJF_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP0950795.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVVVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKJF_003494"
     CDS             complement(14213..15139)
                     /gene="rlmF"
                     /locus_tag="ACHKJF_003494"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP0950796.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15415..15675
                     /gene="mcbA"
                     /locus_tag="ACHKJF_003495"
     CDS             15415..15675
                     /gene="mcbA"
                     /locus_tag="ACHKJF_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP0950797.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            15940..18222
                     /gene="fiu"
                     /locus_tag="ACHKJF_003496"
     CDS             15940..18222
                     /gene="fiu"
                     /locus_tag="ACHKJF_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP0950798.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18264..18941
                     /gene="ybiX"
                     /locus_tag="ACHKJF_003497"
     CDS             18264..18941
                     /gene="ybiX"
                     /locus_tag="ACHKJF_003497"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP0950799.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            19015..19281
                     /gene="ybiI"
                     /locus_tag="ACHKJF_003498"
     CDS             19015..19281
                     /gene="ybiI"
                     /locus_tag="ACHKJF_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP0950800.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            19546..19806
                     /gene="ybiJ"
                     /locus_tag="ACHKJF_003499"
     CDS             19546..19806
                     /gene="ybiJ"
                     /locus_tag="ACHKJF_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP0950801.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
BASE COUNT         4911 a         5204 c         4877 g         4886 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actggctcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctgag catagtaccc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaagttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcag ctcagcaatc tgcgctttct tcttggcgtt atcagccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttgcc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaaa ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt attggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca attcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtattta acttccagat cggccacttt atagccgtct
     1321 tcctcactca tttccggcaa agcatagatg cggtcacgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccaa agtcggctct
     1501 aagtcgccgc cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc aaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgaacc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt ccactcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtgtcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaatttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacctgg tcggcttatt tatttttgcg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagaatgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggtg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg tgcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa acacgaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttctg catctgatgg gctcgcatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcaatcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctctctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aaatatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gctttttctc gcagtggacg ggaattaaag cgaaggaaat
     6481 aaatatcaaa tacccgttta tatctgagaa gaaagccggg ccgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaaacctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctcttgt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctacttttcc
     8461 tctacaccgt ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc
     8521 gagcagataa ataagaattg ttcttatcaa tatatctaac tcattgaatc tttattagtt
     8581 ttgtttttca cgcttgttac cactattagt gtgataggaa cagccagaat agcggaacac
     8641 atagccggtg ctatacttag tctcgttaat tactgggaca taacatcaag aggatatgaa
     8701 attatgagta ccgctaaatt agttaaatca aaagcgacca atctgcttta tacccgcaac
     8761 gatgtctccg acagcgagaa aaaagcaaca gtagagttgc tgaatcgcca ggttatccag
     8821 tttattgatc tttctttgat taccaaacaa gcgcactgga acatgcgcgg cgctaacttc
     8881 attgccgtac atgaaatgct ggatggcttc cgcaccgcac tgatcgatca tctggatacc
     8941 atggcagaac gtgcagtgca gctgggcggt gtagctctgg ggaccactca agttatcaac
     9001 agcaaaaccc cgctgaaaag ttacccgctg gacatccaca acgttcagga tcacctgaaa
     9061 gaactggctg accgttacgc aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg
     9121 aaagatgacg acaccgcaga tatcctgacc gccgcgtctc gcgacctgga taaattcctg
     9181 tggtttatcg agtctaacat cgaataaatc catcgctgat ggtgcagaac tttagtaccc
     9241 gataaaagcg gcttcctgac aggaggccgt tttgttttgc agcccacctc aacgcactta
     9301 tttagtgcat ccatctgcta tctccagctg attaagtaaa ttttttgtat ccacatcatc
     9361 acacaatcgt tacataaaga ttgttttttc atcaggtttt acgctaaata atcactgtgt
     9421 tgagtgcaca attttagcgc accagattgg tgccccagaa tggtgcatct tcagggtatt
     9481 gccctataaa tcgtgcatca cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaac
     9541 tggcacgatt ttttcatata tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa
     9601 aggaaatgct atgaagtctg tattaaaagt ttcactggct gcactgaccc tggcttttgc
     9661 ggtttcttct catgccgcgg ataaaaaatt agttgtcgcg acggataccg ccttcgttcc
     9721 gtttgaattt aaacagggcg ataaatatgt gggctttgac gttgatctgt gggctgccat
     9781 cgctaaagag ctgaagctgg attacgaact gaagccgatg gatttcagtg ggatcattcc
     9841 ggcactgcaa accaaaaacg tcgatctggc gctggcgggc attaccatca ccgacgagcg
     9901 taaaaaagcg atcgatttct ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa
     9961 agctaacaat aacgatgtga aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa
    10021 gagcggtact ggctccgttg attacgcgaa agcaaacatc aaaactaaag atctgcgtca
    10081 gttcccgaac atcgataacg cctatatgga actgggcacc aaccgcgcag acgccgttct
    10141 gcacgatacg ccaaacattc tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc
    10201 ggtaggtgac tctctggaag cgcagcaata cggtattgcg ttcccgaaag gtagcgacga
    10261 gctgcgtgac aaagtcaacg gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga
    10321 aatctacaaa aaatggttcg gtactgaacc gaaataataa cgctacacct gtaaaacgca
    10381 ctggcagttc cctctcccct ctggggagag gattagggtg aggggcgcaa acccgctccg
    10441 gggccattaa ttaccctgaa tttgattatt tacaccacgg taacaggaac aacatatgca
    10501 gtttgactgg agtgccatct ggcctgccat tccgcttctg attgaaggtg ccaaaatgac
    10561 cctgtggatt tcggtcctcg gtctggcagg cggtctggta atcggattgc tggcaggttt
    10621 tgcacgcacc ttcggtggtt ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat
    10681 ccgcggcaca cctatcgtcg tccaggtgat gtttatctat ttcgccctgc cgatggcgtt
    10741 taacgactta cgcatcgacc catttactgc ggcggtggtc accatcatga tcaactccgg
    10801 cgcgtacatt gcggaaatca cgcgtggtgc ggtgctgtct attcacaaag gttttcgtga
    10861 agcaggactg gcgctcggtc tttcacgttg ggaaaccatt cgctacgtca ttttaccgct
    10921 ggcactgcgt cgtatgctgc cgccgctggg taaccagtgg atcatcagca ttaaagacac
    10981 ctcgctgttt attgttatcg gcgtggcgga actgacccgt caggggcaag aaattattgc
    11041 cggtaacttc cgcgcccttg agatctggag cgccgtggcg gtgttctatc tgattattac
    11101 cctggtgctg agctttattc tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa
    11161 tttaaaaacg tctccaagca ttttggtcca acccaggtgc tgcacaatat cgatttgaac
    11221 attgctcagg gcgaagtcgt ggtgattatc gggccgtccg gttccggtaa atcgaccctg
    11281 ctgcgctgca tcaacaaact ggaagaaatc acctccggcg atctgattgt cgatggcctg
    11341 aaggttaacg atccgaaagt tgacgagcgc ctgattcgcc aggaagcggg tatggtgttc
    11401 cagcagtttt acctcttccc gcatctgacg gcgctggaaa acgtcatgtt tggtccgcta
    11461 cgcgtgcgtg gcgcgaacaa agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa
    11521 gtcggtctgg cagaacgtgc acatcactac ccttccgaac tttctggtgg tcaacagcag
    11581 cgtgtggcga ttgcccgcgc gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg
    11641 acttccgctc ttgacccgga actgcgccat gaagtgctga aggttatgca ggatctggct
    11701 gaagaaggga tgacgatggt gatcgtgacc cacgaaatcg gttttgccga gaaagtagct
    11761 tcgcggctga tctttatcga caaaggccgg attgcggaag atggcgatcc gcaggtattg
    11821 atcaagaacc cgccgagcca gcgcttgcag gaatttttgc agcacgtctc ttaacttctc
    11881 tgcctccctt tcacccgaaa gggaggcaca ccagattcct ctcatttaaa atcgcccctc
    11941 ccccagcatc tatacttatc tttttgctct gttttctcac tggaggagtc atgcggtgga
    12001 tcctgttcat cctcttctgc ctgctgggcg cacctgccca cgcggtatcc atacccggcg
    12061 ttacaaccac aacgacaacg gactcaacga ctgaaccggc cccggaaccg gatatcgaac
    12121 aaaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    12181 aattgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    12241 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    12301 gccattatgg tgaagccctt tccgcccgtt tcggacaact ttatcgcaat atcaccggct
    12361 ccccgcataa gccgtttaat ccacaaacct tcagcaatgc gttgacccat ttttcaatgt
    12421 tagcggtatt agtgtttggt ttttactggc tgattcgtct gtgcgcactg ccgctgtatc
    12481 gcaaaatggg ccagtgggcg cggcaaaaaa atcgcgaacg cagcaactgg ctgcagcttc
    12541 cggcgatgat tatcggggcg tttattatcg acctactgtt actggcactg acattgtttg
    12601 tcggccaggt attaagcgac aacctgaatg cgggcagtcg caccatcgct ttccaacaaa
    12661 gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    12721 tttgcccaaa cgtggctgaa ctgcgcccgt tcacgattca ggacgagagc gcccgttact
    12781 ggagtcgtcg cctgagctgg ttaagcagtt tgataggtta tggcctgatt gtggtcgtgc
    12841 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    12901 tgtgcatgac cgtctgggcg ttgtacctga tctttcgcaa taaaaaagag attacccaac
    12961 atttgctcaa ctttgcggag cattcgctgg cctttttcag cctgtttatc cgcgcctttg
    13021 cgctggtgtg gcactggctg gcaagcgcct attttatcgt gctgtttttc ttttcgttgt
    13081 tcgatccggg caacagcctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    13141 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    13201 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    13261 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcggtaatg ttgctgttga
    13321 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    13381 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacagtgc
    13441 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    13501 cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca
    13561 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    13621 ctggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtgaaa gatattatca
    13681 ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggtt actatcgggc
    13741 cgttgaccgg cactgtggaa cggatgtcga ttcgctccgt gggcgtgcgt caggataccg
    13801 gggcgtatca catcattcct tggtcttcga taaccacctt tgctaacttc gtccgcggca
    13861 ttggttcggt ggtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    13921 aggcactgaa agatgcggta gcggaattga tggaaaacga agaaattcgc gggctggtta
    13981 ttggtgaacc aaactttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    14041 tttcgttcac cacgctgcca ctcaaacagt ggacggtccg ctttgccctc gacagccagg
    14101 tgaaaaagca tttcgacctg gcaggtgttc gcgcgccagt gcagacttat caggtgctgc
    14161 ctgctccggg cgcggccccg gctgaaccgc tgccgccggg ggagccaacg ctttaacgtt
    14221 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    14281 tctgcttttg cccctgggcc atctcttttt taaccacctt caccgcgccc acgtccgtca
    14341 gagcacgata caacggcggt aagttttcac cacgagaaac cagcgaggta aaccacatca
    14401 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaaggcg acttcaccac
    14461 cttcacacca caactcctgt tgctggccgc caaagttcag tgcatcgtct ttgttcagcc
    14521 ccaggttacg gcgtttacgc tcactacctg cccgtgccgc agcggcggaa tcgtggaatg
    14581 gcgggttaca caaggtcgcg tcgtattgct cgtttttatg gatgatgccg ttaaaaatcg
    14641 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    14701 tgatcgcctg cgcactactt aacgcctgac tgctggtttc actaccggta aaacgccagc
    14761 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    14821 tgctggcatt agctggaatt gttccgcttg cttctgccag taaatcggca aggtgatgaa
    14881 tataatccgc ccggcccggt actggcgggc agagaaaacc gtccgggata tcccagttcg
    14941 ctacggcgta aaaatgggcc agcaatgcct tattgagcgc cttcaccgcc agcggattgg
    15001 caaagtctac gctttgctcc cctgcgggtg taagcgtgag gaattgcctg agttcaggat
    15061 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    15121 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    15181 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    15241 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    15301 aaaatatatc gttcaaaaaa taagcacatc tctgtcaatt ttttgcgtca ttttcatcgc
    15361 ccaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    15421 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    15481 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    15541 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgagcaa
    15601 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt cggtactgca
    15661 accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct
    15721 gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg
    15781 cgcaatgggt gatagaaaat cgcgccaagt gataatgctt atcaaaatta ttatcacttg
    15841 cacgaacact atcacgagat taacagtggc atcgcatccg cagagaggct ttctcgtggc
    15901 agtgaaaatt tcaacatata aaaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc
    15961 cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg catcacgcct
    16021 gtggctcagg cactcgccgc cgaagggcaa actaacgcgg atgacacgct ggttgtcgaa
    16081 gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg
    16141 gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc
    16201 gcaaccaacc ttaccgacgc gctcaaaaac gtccccggcg tgggtgcgtt ttttgcgggt
    16261 gagaacggta actccaccac tggcgacgcc atttatatgc gcggtgctga tacctctaac
    16321 agtatttata ttgatggcat tcgcgatatc ggcagcgtct cgcgcgacac cttcaatact
    16381 gagcaggtcg aagtgattaa agggccttcc ggcaccgact acgggcgcag cgcgccgacg
    16441 ggctcgatca atatgatcag caagcagccg cgcaatgatt ccggcattga cgcctccgcc
    16501 agtattggca gcgcctggtt ccgccgcggc acgctggacg tcaatcaggt cattggtgat
    16561 accactgcgg tgcgcctgaa tgtgatgggc gaaaaaacgc acgatgccgg acgcgacaaa
    16621 gtcaaaaatg agcgttacgg cgtcgcccct tctatcgctt ttggccttgg tacagcgaat
    16681 cgtttgtatc ttaattatct gcatgtcacc cagcacaaca cgccagacgg cggcattccg
    16741 accatcggtt tgccgggtta ttctgcccca tctgcgggaa cagcggctct gaatcattcc
    16801 ggaaaagttg atactcataa cttttacggc acggattccg attacgacga ttcgaccacc
    16861 gacaccgcca ccatgcgttt tgagcacgac atcaacgata acaccaccat tcgcaatact
    16921 acccgttggt cgcgcgtaaa gcaggattac ctgatgacgg cgattatggg cggggcgtcg
    16981 aatattactc agcccaccag cgatgtgaat agctggacct ggtcacgcac ggcgaatacc
    17041 aaagatgtga gtaataaaat tctcaccaac cagaccaacc tgacctcgac gttctatacc
    17101 ggttctatcg gtcatgatgt cagtaccggc gtggaattta cccgtgaaac gcagactaac
    17161 tacggcgtta atccggtgac gttacccgcg gtaaatattt atcatcctga cagcagcatt
    17221 catcccggcg gcctgacgcg caacggcgca aacgccaatg gtcagacgga taccttcgca
    17281 atttacgcct tcgatacgct gcaaatcacc cgtgattttg agctgaacgg cgggatccgt
    17341 ctggataatt atcatactga atatgacagt gccaccgcct gcggcggcag cggacgcggt
    17401 gccatcacct gcccagctgg tgtggcaaaa ggttctccgg tcaccaccgt cgacaccgcc
    17461 aagtcgggca atctggtgaa ctggaaagcc ggggcgctgt atcacctgac ggaaaacggt
    17521 aatgtctata ttaactatgc cgtttcccag cagcctccgg gcggcaacaa cttcgccctt
    17581 gcgcagtctg gcagcggtaa cagtgccaac cgcaccgatt ttaaaccgca aaaagccaac
    17641 accagcgaga ttggcaccaa atggcaggtt ctggataaac gcctgttgct caccgccgcg
    17701 ctattccgta ctgatatcga aaatgaagtt gagcaaaatg atgacggaac ttactcgcaa
    17761 tacggtaaga aacgtgtcga aggctatgag atatccgtgg ccgggaatat cacgcccgcg
    17821 tggcaggtga ttggcggcta tacccagcaa aaagcaacca tcaaaaacgg caaagatgtt
    17881 gcccaggatg gttcctcatc gctgccgtat accccggagc acgccttcac cttatggagc
    17941 cagtatcagg caacagatga tatctctgtt ggcgcgggcg cacgctatat cggcagtatg
    18001 cataaaggtt cagacggcgc ggtgggaacg ccagcgttta ccgaaggtta ctgggtcgcc
    18061 gatgccaaac tggggtatcg ggttaatcgc aatctcgact tccagctaaa cgtttacaac
    18121 ctgtttgata ccgattacgt cgcctcaatc aacaagagcg gctaccgtta tcacccgggc
    18181 gagccaagaa ccttcttgct cacagccaat atgcatttct gattcagatg tggggcgcag
    18241 gccccacttt ttggagaact tgtatgatgt accacattcc cggcgtgtta tcgccacagg
    18301 acgtcgcccg gtttcgcgaa caactggaac aagccgaatg ggtggatgga cgcgtcacca
    18361 ccggcgcaca aggtgcgcaa gttaagaaca atcaacaggt cgacacccgc agcacgttat
    18421 acgccgccct gcaaaatgag gtgctgaacg cggttaacca acatgcttta ttctttgccg
    18481 cggccttgcc gcgtaccctt tccacgccgc tgtttaatcg ctatcagaac aatgaaacct
    18541 atggtttcca tgtggatggc gcagtacgca gccatccaca aaacggctgg atgcgtactg
    18601 acctttctgc cacgctgttt ttaagcgatc cacaaagcta cgacggcggc gaactggtcg
    18661 ttaatgacac cttcggacaa catcgggtaa aactcccggc aggcgatctc gtgttgtatc
    18721 cctccagcag cctgcattgc gtgacacccg taacccgcgg cgtacgagtg gcatcattta
    18781 tgtggatcca gtcgatgatc cgtgatgata aaaagcgcgc catgctgttt gaactggaca
    18841 acaatattca gtcgctgaaa agccgctatg gtgaaaatga agagatcctg tcgctgctta
    18901 atctttatca taatctgctg cgggaatggt cggagatctg atgcttgaac tgcccaagtt
    18961 cgtccctgga ttagctacac ttaactgtac aagcattgat atggggagga cgatatggca
    19021 tccggttggg ctaacgatga cgccgtcaac gaacagatca acagtacaat tgaagatgcg
    19081 attgcccgcg ctcggggtga aattccgcgt ggcgaaagcc tggatgaatg tgaagagtgc
    19141 ggtgccccca tcccgcaagc ccgtcgggaa gccattcctg gcgtgcgctt atgcattcat
    19201 tgtcagcagg agaaagattt acaaaaacca gcgtatacag gatataatcg cagaggttcg
    19261 aaagacagcc agttacgtta actacaactg gcgagatgta tagcgagtat aatttgtatt
    19321 ttgcgtggca ttccgtgcct ttaacgctgt cgtgactgcc acgctgcata aaacaagtcc
    19381 ataagtacaa caaataaatg gtttatcagt atgttagata tcaatcaatt tatttgaaca
    19441 aggcggtcaa ttctcttcga ttttatctct cgtaaaaaaa cgtgatactc atcacatcga
    19501 cgaaacaacg tcacttatac aaaaatcacc tgcgagagat taattatgaa aactatcaat
    19561 actgttgttg ctgctatggc tctttcaact ctgtcatttg gcgtgtttgc cgcggaaccg
    19621 gtaacggcat cccaggcaca gaacatgaat aaaatcggcg tggtttctgc cgatggcgca
    19681 tccaccctcg atgccctgga agcgaaactg gctgagaaag ccgcggcagc cggtgccagt
    19741 ggatacagca tcacttccgc caccaacaac aataaattaa gcggtactgc ggtaatttat
    19801 aagtaattcg taggaaggat aaggcgttta cgccgcatcc ggcaacagat gcctgatgcg
    19861 acgctactgc gtcttatc
//
LOCUS       DAZGXZ010000094        19825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000094 DAZGXZ010000000
VERSION     DAZGXZ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.094"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(99..803)
                     /locus_tag="ACHKJF_003500"
     CDS             complement(99..803)
                     /locus_tag="ACHKJF_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0950802.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(940..1392)
                     /gene="moaE"
                     /locus_tag="ACHKJF_003501"
     CDS             complement(940..1392)
                     /gene="moaE"
                     /locus_tag="ACHKJF_003501"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP0950803.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1394..1639)
                     /gene="moaD"
                     /locus_tag="ACHKJF_003502"
     CDS             complement(1394..1639)
                     /gene="moaD"
                     /locus_tag="ACHKJF_003502"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP0950804.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1632..2117)
                     /gene="moaC"
                     /locus_tag="ACHKJF_003503"
     CDS             complement(1632..2117)
                     /gene="moaC"
                     /locus_tag="ACHKJF_003503"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP0950805.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2120..2632)
                     /gene="moaB"
                     /locus_tag="ACHKJF_003504"
     CDS             complement(2120..2632)
                     /gene="moaB"
                     /locus_tag="ACHKJF_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP0950806.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2654..3643)
                     /gene="moaA"
                     /locus_tag="ACHKJF_003505"
     CDS             complement(2654..3643)
                     /gene="moaA"
                     /locus_tag="ACHKJF_003505"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP0950807.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3631..3775)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4040..4948
                     /gene="yvcK"
                     /locus_tag="ACHKJF_003506"
     CDS             4040..4948
                     /gene="yvcK"
                     /locus_tag="ACHKJF_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP0950808.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5140..7161)
                     /gene="uvrB"
                     /locus_tag="ACHKJF_003507"
     CDS             complement(5140..7161)
                     /gene="uvrB"
                     /locus_tag="ACHKJF_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP0950809.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7740..8417)
                     /gene="bioD"
                     /locus_tag="ACHKJF_003508"
     CDS             complement(7740..8417)
                     /gene="bioD"
                     /locus_tag="ACHKJF_003508"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0950810.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(8410..9165)
                     /gene="bioC"
                     /locus_tag="ACHKJF_003509"
     CDS             complement(8410..9165)
                     /gene="bioC"
                     /locus_tag="ACHKJF_003509"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP0950811.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            complement(9152..10306)
                     /gene="bioF"
                     /locus_tag="ACHKJF_003510"
     CDS             complement(9152..10306)
                     /gene="bioF"
                     /locus_tag="ACHKJF_003510"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP0950812.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            complement(10303..11343)
                     /gene="bioB"
                     /locus_tag="ACHKJF_003511"
     CDS             complement(10303..11343)
                     /gene="bioB"
                     /locus_tag="ACHKJF_003511"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP0950813.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11430..12719
                     /gene="bioA"
                     /locus_tag="ACHKJF_003512"
     CDS             11430..12719
                     /gene="bioA"
                     /locus_tag="ACHKJF_003512"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP0950814.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12778..13254
                     /locus_tag="ACHKJF_003513"
     CDS             12778..13254
                     /locus_tag="ACHKJF_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP0950815.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13406..14689
                     /locus_tag="ACHKJF_003514"
     CDS             13406..14689
                     /locus_tag="ACHKJF_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP0950816.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(14831..17092)
                     /locus_tag="ACHKJF_003515"
     CDS             complement(14831..17092)
                     /locus_tag="ACHKJF_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP0950817.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTMETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFIDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(17275..18708)
                     /locus_tag="ACHKJF_003516"
     CDS             complement(17275..18708)
                     /locus_tag="ACHKJF_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP0950818.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(18784..>19825)
                     /locus_tag="ACHKJF_003517"
     CDS             complement(18784..>19825)
                     /locus_tag="ACHKJF_003517"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP0950819.1"
                     /translation="PCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGNDLEID
                     GIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGAFAIE
                     NGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVALTFL
                     NAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELDADKA
                     LLARIESIRLQAGKAMGLGDVSNMVIPKPVLISLAQKGGAINVRYFMPHSCHRALAIT
                     GAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRASVIRT
                     TRKIFSGEVYLP"
BASE COUNT         4730 a         5342 c         5001 g         4752 t
ORIGIN      
        1 gccataaggc gtattttata cttggtactt gttaagtgtc gtttttagta agaaaaaaaa
       61 tcccgacaca tggccgggat attgatggtg aaaaagaatt aacggcggtt gccaaaaatt
      121 cgcagcaaca tcaggaacag gttgatgaag tccagataca aggttaacgc gccaagaatg
      181 gaatatttgc gcaggttcga cgtgtcgcgg gtatcaatct gctcacccat atttttcaat
      241 ttctgagtgt catacgccgt caatccgaca aagacaatca cgccgatgta ggtaactgcc
      301 cacatcaatg cttcgctttt cagccagaag ttgaccagcg atgccagcac aatgccgatt
      361 aacgccataa acagcatatt gccaaagcca cttaaatcgc gcttcgtggt gtaaccgtac
      421 aggctcattg cgccgaacat cccggcagta acgacgaaag tactggcgat agaagcagcg
      481 gtatagacaa tgaatatact ggaaagcgta agacccgtca gcgccgaata aagcataaag
      541 agcatcgtcg ttacacctgc gctcagcttt tgaatcatcg ctgataacac aataaccaat
      601 gctaattgcg cgatgatcag accgattaaa aagacacggt tagtaaacaa cagctccatc
      661 acggccgcgg aattagccgc ataccaggca acaaatgcgg tcagcaacaa gccaacggtc
      721 atccagccat agacttgcgc catataagtt tgcaagccag cccggggttg tacgattgaa
      781 tcagaacgtg ggaatctgtc catgacgatc tcctgaagat ataaggaata tcttaaggat
      841 actgcaaaat gatgaggctg tgcatcgacg cagcgtaaac gcatgtactg agcggtgaaa
      901 ttgccggacg cagcggtgcc ttatccggct aacaaaaaac taccagcgtt ttgccgcctg
      961 ctgatcgctc tcccgagctt caacccagcg gtcgccttcc ggcgtggctt cgcgcttcca
     1021 gaacggtgcg cgggttttga gataatccat aataaactgc ccggcttcaa acgcactgct
     1081 gcgatgcgca ctggtgacac cgacaaaaac gatttcatcg cccggccata attccccgat
     1141 gcggtgaatc acagtgacgc gccccagcgg ccagcggtta cgcgcttcat caacaatttc
     1201 tgccagtgct ttttcagtca tccccggata gtgttcgagg gttaatgctt tgacgctgtc
     1261 gcccaggtta tggttgcgca ccttaccagt aaaggtgact accgcaccgt cttcgtcacg
     1321 ctccgccagc cacgggtact cttctcctac gctgaacggc tgcggaccaa caacaatttt
     1381 ggtttctgcc atcttaacct ccggttaccg gcgggaagaa agctacttcg tcgccgtcag
     1441 tcagcggatg gtcaaaactc accagcgtct ggttgacggc agccagtaat ttgccatctt
     1501 ccagcgccag cgcccagcga tcgctctgcg cagccatgtg ctggcgtaac gcttcaacag
     1561 ttgggaaatc cgcagccact tcggttgcat ctgttcccac caactcgcgc acctgggcga
     1621 aaaaaagaac tttaatcatc cgcttccacc ttaaagtcac ccgacttgcc gccgctcttc
     1681 gccagcaaac gtaccggacc aatcaccata tctttttgca ccgctttgca catgtcataa
     1741 atggtcagcg ccgccacgga ggccgcggtt aatgcttcca tttcgacacc ggttttcccg
     1801 gtcaggcggc ataaggtttc tatacgcacc cgattgtgct ccggctcggc ctgtaaattg
     1861 acttcaactt tgctgagcat cagcggatga cagagcggga tcagatccca ggtgcgtttt
     1921 gccgcctgaa taccggcaat acgcgcagtg gcaaatacgt cgcctttgtg gtggcgacca
     1981 tcaataatca tcgccagcgt ctcgctgcgc atggtgacaa aggcttccgc ccgcgcttca
     2041 cgcacggttt ccgctttggc ggagacatcc accatgtgcg cttcgccagc ggcgttgata
     2101 tgggtcagtt gcgacatact tatttcttca aatgtggatg gaaattacac ggacgcgtac
     2161 gggcatccag ctgcggcgcg atgatatttt cccatgcggt acggcacgct ttggtcgaac
     2221 ccggcatggc gaaaatcagc gttttgttgg cgacgcccgc taccgcacga gattgcaacg
     2281 tggaagtgcc aatctcttca aacgacaaca tccggaacac ttcaccaaaa ccttcaattt
     2341 cacggtcgaa caacggcagc aatgcttcgg gagcctgatc accttccgtc aggccagtac
     2401 cacccgtaat caataccact tgtacatcgt cgctggcgat ccacgcagat acctgagcgc
     2461 gaatagcgta gcggttttct ttcacaatgg ctttatcgac aacgtgatgg cccgcttctt
     2521 gcgccgaatc gcgcagatag tgaccggagg tatcgtcttc ttcaccgcga cgattagaaa
     2581 ccgtaagaat agcaatacgg gtcgggataa attcagtgct tacctgactc atctgatctc
     2641 tccttttgac gttttagccg ccaatgtacg ataagttttg cgtaataccg gtgttgtttt
     2701 gatgcaggaa atgggtctgt ttcttctccc gcagcgccgc tgaaatacgc gcttccagcg
     2761 cctgttgctg ggtatcgtct tccagcagat cgcgcaggtt aacgccgcct tcaccaaaca
     2821 ggcagagatg gagtttacca atggaggaaa cgcgcaggcg gttgcaagtg gcgcagaagt
     2881 ctttttcata cggcatgata aggccaatct ctccggcgta atctggatgg caaaagactt
     2941 gcgcgggacc gtcgctgcgt tgacgtaatt ggtggatcca gccgcgacgc agtagctcgt
     3001 cacgcagaac ctgaccagag atgtgatgct tacggaagag ctcgctgccc tcgcccgttt
     3061 ccatcagttc gatgaaacgc agctggatag ggcgatgctg gatccagttc agaaaggtgt
     3121 cgagctggtg atgattaaca tcacgcatca gcacggtatt gactttgacc ttctcaaaac
     3181 cggcctcaaa tgcagcatca atccccgcca tgacctggtt gaatttatcc tgcccggtaa
     3241 tagcgtgaaa ctggcgggcg tccagactgt cgacactgac gttaatgcca gtaagtcccg
     3301 catcgcgcca gttcgccaca tcgcgttcca gacggtaacc attggtggtg accgcaatct
     3361 ggcggatagc gtcgttttcc cgcacagcgg cgatgatatc ggtaaagtcg cggcgtaaag
     3421 acggctcacc gcctgtcagg cgcacttttt cggtgcccag actggcgaag gcgcgcgtaa
     3481 cccggcgaat ttcatcgacg gtaagaaagc ctttattggt gacgccgctc ggtttgtagc
     3541 catccggcag gcagtaggtg caacgaaagt tacacacatc ggtaatcgac aggcgcaagt
     3601 agtaaaactt acgcgcaaat gcatcagtca gttgtgaagc catgtacacc tttccagata
     3661 cgggaggcga agtcatttct tccttcgccc tggtggcaat ctttacgagc attgtcacgg
     3721 ccaaagcacc gtatcagttg acccaggtgc agaggctaga gtgtttagtg gttatgccga
     3781 tactagcgtg taaatgagat ttttaccatc cacactttcg ctatgtaatc atgtatatag
     3841 cgtcatgatc gtgctatttc gctacagaat aagggaaaat ggcggcttta ctttgatata
     3901 catcatcagg gaggcatagc gtgcatcgtc ctttagaggt agaagaaaat agtttgtatc
     3961 ctcaatattg gcaggttaat tgctgtttcc ccgcaatttg cgctactgta gcgcggataa
     4021 tctgactcca ggaaactata tgcgcaatcg tacgctggct gatcttgatc gtgtcgttgc
     4081 tctcggcgga gggcatggac tgggacgcgt tctctcatca ctttcgtctt tgggttctcg
     4141 tttaacgggt atcgtcacca ccaccgataa tggtggctcg acggggcgta ttcgccgttc
     4201 agaaggcggc attgcctggg gcgatatgcg caactgcctc aaccagctga taacggaacc
     4261 gagcgtcgcc tccgcgatgt ttgaataccg ttttggtggc aatggcgaac tttccggtca
     4321 taatctcgga aacttgatgt taaaggcgct ggatcacctt agcgtgcggc ctctggaagc
     4381 catcaattta attcgtaatc tgctgaaagt ggatacgcat ttgattccaa tgtcagagca
     4441 tcctgttgat ctgatggcga ttgacgatca ggggcatgaa gtttacggcg aggtcaatat
     4501 cgaccagtta actacgccga ttcaagagtt attgttaacg cctaatgtac ccgcaacgcg
     4561 tgaggcggtt cacgctatca atgaagcgga tctcatcatt attgggcctg gcagttttta
     4621 taccagcctg atgccaattc tgctgctgaa ggaaatcgcc caggcattac gccgcacgcc
     4681 agcgccgatg gtttatatcg gcaatctggg gcgtgagttg agtttacctg cggctaattt
     4741 gaagctggaa agcaagctgg caattatgga gcagtatgtt ggtaaaaagg tcattgatgc
     4801 ggtcatcgtc gggccaaaag tggatgtctc ggcggtgaaa gagcggattg tgatccagga
     4861 ggtactggag gccagcgata ttccgtatcg tcatgaccgc cagttgttac ataacgcgct
     4921 ggaaaaggcg ttacaggctt taggttaacg gaaatccgaa ggaaaattcc ggcttcctat
     4981 tgaagacaaa gtgcgcgttg tttatgccgg atgcggcgtg aacgccttat ccggcctaca
     5041 aaccgcgcaa attcaatata ttgcggagaa aatgtaggcc tgataagcgt agcacatcag
     5101 gctgttttcc gtttgtcatc agtcttcttc gctatcctgt tacgatgcgg caataaacaa
     5161 ctcacgcaac tgatgcaact ggtcacgaat ttgtgccgct tcttcgaact ccagattctg
     5221 cgcgtgttgc atcatcagcc cttccagttc atggattttc tgctgcaacg ctttcggcga
     5281 catatccatc ggcacattat ccggctcaac aatcgggcgc gattttcctc tgcccttcgc
     5341 tttggttttg gcaatgttct gccccagcgc caggatatcg accactttct tgttcaagcc
     5401 ttgcggcgta atgccgtgtt cctcgttgta cttctgctgt ttctcacggc gacgttcggt
     5461 ttcgccaatc gctttcgcca ttgatggggt gatcttatcg ccgtagagaa tcgctttacc
     5521 gttaacgtta cgtgccgcac gaccaatggt ctggatcaac gaacgttcgg aacgcaggaa
     5581 gccttctttg tcagcgtcga ggatcgccac cagcgacact tccggcatat ccagaccttc
     5641 gcgcagtaag ttgatcccta ccagcacgtc gaactcaccc agacgcaagt cgcggataat
     5701 ctccatacgt tcgacggtgt cgatatctga gtgaagataa cgcacgcgct cgccatgttc
     5761 ttcgagatat tcggtaagat cttccgccat ccgcttggtc agtgtggtga ccagtacgcg
     5821 ttcgttaatt gccgctcgct gacgaatctc cgaaagaaga tcatcaacct gtgtcgccac
     5881 cggccgcact tcgataatcg ggtcaagcaa tccggttgga cgcaccacct gatccaccac
     5941 atcgccgccg gatttttcca gctcgtaatt acccggcgtc gccgaaacat agatggtttg
     6001 cggcgctaat gcttcgaact cttcaaactt aagcggacgg ttatccagcg ctgatggcag
     6061 gcggaagccg tactccacca gtgtctcttt acgcgcccgg tcaccgcgat acatgccgcc
     6121 aatttgtgga atggtgacat gagattcatc gaccaccagc agcccatcgg cagggaggta
     6181 atcaaacagc gtcggcggtg gctcacccgg tccacgaccg gagaggaagc gcgagtagtt
     6241 ttcaatcccc gaacagtagc ccagctcgtt catcatctcc agatcaaact gggtacgctg
     6301 ggtcagccgc tgctcttcca gcagtttgtt gttttccaat agcactttgc gtctggcggc
     6361 cagctcttct ttgatctcct ccatcgcctg tacgatgcgc tcgcgcggtg tgacgtagtg
     6421 cgttttcggg tagatggtaa aacgtggaat ggtggaaaca atctgcccgg ttagcgggtc
     6481 aaataacgac aatcgttcca cttcctcgtc aaacagttcc acgcgaagtg caatgtcatc
     6541 cgattctgcc gggaagatat caatcacctc gccacgaacg cggaaagtac cacgctggaa
     6601 tgcttgatca ttacgagcgt attgcagctc cgccagtcgg cgcagaatcg cgcgctgatc
     6661 gataatcata ccgaccgtga gatggagcat catcttgaga tataaatcag gatcgcccag
     6721 accataaatc gcggaaacag acgccactac aaccacatca cgtcgctcca gcatcgcttt
     6781 ggtggcggat aaacgcatct gctcaatatg ttcgttaacc gaggcatctt tctcaatgaa
     6841 ggtgtcggaa ctcggtacat aggcttccgg ctgatagtag tcgtagtagg agacgaaata
     6901 ctccaccgcg ttttccggga agaactcttt catttcgcca tacagctggg ccgccagcgt
     6961 tttgttgggc gcaagtacca tggttgggcg ctgaaggtca gcaatgacat tggcaatggt
     7021 gaaggttttc ccggagccag tcacgccaag taacgtctgg tgcgccaggc catcttccag
     7081 cccctcttcg agacgtcgaa tcgcctctgg ctgatcgcca gaaggtttaa aagcggaatt
     7141 cagtttgaac ggtttactca tgagtcgcta cctgaaggag ttgggcgggc aggtatgtaa
     7201 ttttactcgt cgtacttaat tatgccaaca aattatactg gataaaaaaa cagttcatca
     7261 ccataatatt tctgttacag cgtaaactcc gctgtcaatc atgagcaaaa tttactctgt
     7321 ggcgagataa aactccggcc ttaccgggtt atccccaaag caacggcttt tttaacattt
     7381 gtcaagatga gtgatgacag ttttatggca agagatgcct gttcagtgac tgccattgca
     7441 tttatctaac cagttaaaaa ataaaagata tttctttgag ccgtctttaa cgccaattcg
     7501 cgtgcaagcc gcgtattctc tcgcttgcct cgtgttttct aactctaata cacatggtta
     7561 tccacaggaa tagtggataa ctgtctccag cccctatccc ccgccgcctg ggcaattccc
     7621 acattcgccg catgaggcgg aagtaaaatt tttttcgtga ttattttaga atttaattgg
     7681 ttaaattgca gtcaatcgaa gacgcgatct cgctcgcaat ttaaccaaat acaggatggc
     7741 tacaacaagg caaggtttat gtactttccg gttgccgcat tttctggatt ttctgcaagc
     7801 caggggatct ctcccagcag cggcgcggga atcatgcggg tgagcgtggt catatattca
     7861 gcgtgacgtt ttcccggagg cgtaacatcg ttcgccaccc aacccgccag agtcagtccg
     7921 gcgtgttgta ttgcctgcgc agtcaacatc gcgtgattaa tacagccgag tttcacacca
     7981 actaccagta tcaccggcag ttgttcctgt gttacccaat ctgcaaaagt gaaagtgtca
     8041 gaaagcggcg taaaccagcc gccagcacct tccactaaca cccagtcagc ctgttgttca
     8101 agcgcgcgta atccggcgct cattaccaat gattctatcg gcctgccctc ttgcgcgctg
     8161 atgatgtgcg gcgaggtggg ttctgcgaag gtgtaaggat ttaccgttgc gtaatccagc
     8221 tgcaggctgc tgttgtgctg taacgccagc gcgtcactat tacgtaagcc ttccggggtc
     8281 ttttcgctgc cagaggcgac cggtttataa cctgccgtcc gataacctac tgcctttgcg
     8341 gcttgtaaaa gtgcacaact ggcgacagtt ttccccactt cggtatccgt tccggtgaca
     8401 aaataacgtt tactcacgag caatcactcc caaaaaaaga tgatacgtca ggggatatcg
     8461 cccctgtttt tgtggccagg ccagttgcaa tcgctgcaac tgcgaacgcg ttaatattcg
     8521 cgggtcgcgc ccttcatgaa gatgcgtggc tccaatgcct ttcagcgaac gcatggcact
     8581 gagcgcatca tcaaaccaca gcgtgatggg ctgaatatga tgttgataat gcacgccgtt
     8641 cagtgactgt tcgattgcac ctggtggtaa aaagcgatta gcatgcggac gctcgtccac
     8701 tgcctgccac gcctgatgca gttcgggtaa cgatccctgc accagcgtgg taaacgcgac
     8761 cacgcctccg gggcgcacca cccgatacag ctcgcggagt gccgtggata aattaccgca
     8821 ccactgcacc gcgagattgc tccatgcaag atcgaacgtc gcagtcgcta acggcaggga
     8881 ttcgatatct cccgccagat aatggtctgc ggcatccttc tggcgtgcct gaacaagcat
     8941 tggcggcgag agatctaacg ccgtcacctg cgcgtgacgc tctcgccagt ggcggctcat
     9001 ccagcctggc ccacaccccg cgtccagtac gtgggtgtat ttacgctgtg ggagcattgc
     9061 cagtaagacg tcagcactct ggcgctgtag atctgcgtgt tgctcatagt gcgcggctgc
     9121 ccgaccaaat gccgctgcaa tggcttgttt attaaccgtt gccatgcagc atctccagca
     9181 aacggtcaat atcctgcatt tcatgggcgg cggttaaggt caggcgtagc cgcgcggtgc
     9241 cagcgggtac ggttggcggg cgaatcgccg tcacccagca accttgctgg cgcagttttt
     9301 ctgccagctg taacgcacgg ctgttatcac cgacgatcaa tggctggatg gcgctacgtg
     9361 aatccgcaag cgtaaacggc gaaccctgca ctccggcacg aaaacgcgta atgagtgccg
     9421 ccagtttttc gcgccgagcg tccccctctt cactgcgaat gaccgccagc gacgcacgta
     9481 atgcctgcgc ctgagcgggc ggcatactgg tgctgtagat aaggtggcgg gcgaattgca
     9541 gcagataatc cgccaccgta ctggagcaaa gcaccgctgc cccgctgacg ccaaatcctt
     9601 tgccaaaagt cactaccagc aattctggtt ttaccttttg cagccagcag ctgccgcgcc
     9661 cctgctcccc gataacgccc gtgccgtggg catcatcgac catcaaccag ccattgtgct
     9721 gttgcgttac ctgctggatt tccgccagtg gcgcactatc gccgtccatg ctgaacacgc
     9781 cttctgtcac caccatttgc tgccccggac agggggaagc aagcaatcgc gccaaatgag
     9841 tgacatcgtt atgagcaaaa cggcgaagct gcgacgggct taaactggca gcttccagca
     9901 atgaggcatg gctaagccgg tcggcagcaa tacggtcctc tttcgccatc atcgcggcaa
     9961 taactgcctg attagcggca aaaccagaga taaatagcag tgcccgcgaa tagcccagcc
    10021 actcggccag ttcttcttcc agcgcctgat gcgcaacgct ataaccgctg acgtgaccgg
    10081 aaccgccgct accgacgcca aattgctccg caccctgctg ccaggcacgg ataatttgcg
    10141 gatgatggct taaacctaaa taatcgttac tggaaaagtt cagatactgg cgatcatccg
    10201 ccaccagcca gcgtccggct ccttgcgcca ctggataacg gcgacgcagg gcatcggcag
    10261 cacgccgcgc atcgagcgcc gcgttgattt tctcctgcca gctcataatg ctgccgcgtt
    10321 gtaatattcg tcggtgtccg gggtcatcag cgcctgttca agacgttgct gttgttcgtt
    10381 atcccctgcc agcacggcag tttgctgcgg atttagcccc agtttgcgga acagttgcag
    10441 gtctttatct tcttccggat tcggcgtggt cagcagtttg caaccgtaga aaatcgagtt
    10501 tgcgcctgcc ataaagcaca tcgcctgagt ctgttcgttc atctgctcac gtccggcaga
    10561 aaggcgcacg taagaggttg gcatcatgat ccgcgcgacc gcaatggtgc gaataaaatc
    10621 aaaggcatcg acatcatcgt tatcggcaag cggcgtgcct ttcaccttca ccagcatgtt
    10681 gattggtacg ctttccggcg gcgtcggcag gtttgccagt tgcagcaata atccggcgcg
    10741 atcttttacc gtttcgccta agcccacaat gccgccagaa cagactttga tcccggcatc
    10801 gcgcactttt tccagcgtat cgaggcgttc ctgataagtg cgggtggtga tgatattgcc
    10861 gtaaaactcc ggcgaggtgt ccaggttgtg gttgtagtaa tccagcccgg cattcgcaag
    10921 gcgctgcgcc tgagattcac tcaacgtgcc cagcgtcata cacgcctcca gccccatcgc
    10981 ttttaccccc tgcaccattt gttccaggta cggcatatcg cgttcgtggg gattcttcca
    11041 cgccgcgccc atacagaaac gcgtcgatcc cgcagctttc gctttgcgcg ccgactccag
    11101 cacctgttca acttccatca accgctcggc ttccagcccg gttttgtagc gcgagctttg
    11161 cgggcagtat ttgcaatctt ccggacaagc tccggtctta atcgacagca aagtgctgac
    11221 ctgcacctga cgaggatcga aatgctgtcg atgcacctgc tgcgcttcaa acagcagatc
    11281 cagcaacggt ttttcaaata attctgtgac ttgcgacaat gtccagcgtg ggcggtgagc
    11341 catggggctt ctccaaaacg tgttttttgt tgttaattcg gtgtagactc gtaaacctaa
    11401 atcttttcaa tttggtttac aagtcgatta tgacaacgga cgatattgcc tttgaccaac
    11461 gccatatctg gcacccatac acatccatga cctcccctct gccggtttat ccggtggcga
    11521 gcgccgaagg ttgcgagcta attttgtctg acggcagacg cctggttgac ggtatgtcgt
    11581 cctggtgggc ggcgatccac ggctacaatc acccgcagct taatgcggcg atgaagacgc
    11641 aaattgatgc catgtcgcat gtgatgtttg gcggtatcac tcatgcgcca gccattgagc
    11701 tgtgccgcaa actggtggcg atgacgccgc aaccactgga gtgcgttttt ctcgcggact
    11761 ccggttccgt agcggtggaa gtggcgatga aaatggcgtt gcagtactgg caagccaaag
    11821 gcgaagcgcg ccagcgtttt ctgaccttcc gcaatggtta tcatggcgat acctttggcg
    11881 cgatgtcggt gtgcgatccg gacaactcaa tgcacagcct gtggaaaggc tatctgccag
    11941 aaaacttgtt tgctcccgcc ccgcaaagcc gcatggatgg tgaatgggat gagcgcgata
    12001 tggttggttt tgcccgcctg atggcggcgc atcgtcatga aatcgcagcg gtgatcattg
    12061 agccgattgt ccagggcgca ggcgggatgc gcatgtacca tccggaatgg ttaaaacgaa
    12121 tccgcaaaat gtgcgatcgc gaaggtatct tgctgattgc cgacgagatc gccaccggat
    12181 ttggtcgtac cggcaaactg tttgcctgtg aatatgcaga aatcgcgccg gacattttgt
    12241 gcctcggtaa agccttaacc ggcggcacaa tgaccctttc cgccacgctc accacgcgcg
    12301 aggttgcgga aaccatcagt aacggcgaag ccggttgctt tatgcacggg ccaactttta
    12361 tgggcaatcc gctggcctgc gcggcagcaa acgccagcct ggcgattatc gaatccggcg
    12421 aatggcagca gcaggtggcg gctattgaag tacagctgcg cgagcaactt gcccccgccc
    12481 gtgatgccga aatggttgcc gatgtgcgcg tactgggggc aatcggtgtg gtcgaaacca
    12541 ctcgtccggt gaatatggcg gcgctgcaaa aattctttgt cgaacagggt gtctggatcc
    12601 ggccttttgg caaactgatt tacctgatgc cgccctatat tattctcccg caacagttgc
    12661 agcgtctgac cgcagcggtt aaccgcgcgg tacaggatga aacatttttt tgccaataac
    12721 gggaagtcag cgtgagggtt tctggctaca ctttctgcaa acaagaaagg agggttcatg
    12781 aaactcatca gtaacgatct gcgcgatggc gataagttgc cgcatcgtca tgtctttaac
    12841 ggcatgggtt acgatggcga taatatttca ccgcatctgg cgtgggatga agttcctgcg
    12901 ggaacgaaaa gttttgttgt cacctgctac gacccggatg cgccaaccgg ctccggctgg
    12961 tggcactggg tagttgttaa cttacccgct gatacacgcg tattaccgca agggtttggc
    13021 tctggtctgg tagcaatgcc agacggcgtt ttgcagacgc gtaccgattt tggtaaaacc
    13081 ggatacgatg gcgcagcacc gccgaaaggc gaaactcatc gctacatttt taccgttcac
    13141 gcgctggatg tagaacgtat tgatgtcgat gaaggtgcca gcggcgcgat ggtcgggttt
    13201 aacgttcatt tccactctct ggcaagcgcc tcgattactg cgatgtttag ttaatcactc
    13261 tgccagatgg cgcaatgcca tctggtatca cttaaaggta ttaaaaacaa ctttttgtct
    13321 ttttaccttc ccgtttcgct caagttagta taaaaaagca ggcttcaacg gattcatttt
    13381 tctatttcat agcccggagc aacctgtgaa cacattttca gtttcccgtc tggcgctggc
    13441 attggctttt ggcgtgacgc tgactgcctg tagctcaaca ccacccgatc aacgtccttc
    13501 tgatcaaacc gcgcctggta cctcttctcg cccgattctg tcggcaaaag aagcgcagaa
    13561 tttcgatgct caacactatt ttgcatccct gacaccaggt gctgcagcgt ggaatccttc
    13621 cccgattacc ctgcctgcgc aacctgactt tgttgtcggc ccggcgggta ctcaaggtgt
    13681 aacgcatacc acgattcagg cggcggtaga tgcggcaatt atcaagcgca ctaacaagcg
    13741 ccagtatatt gccgtgatgc ctggtgagta tcagggaacg gtgtatgtcc ctgctgctcc
    13801 gggtggaatt actctgtacg gtacaggtga aaaaccgatt gatgtgaaga ttgggctttc
    13861 ccttgatggg ggcatgagcc ctgccgactg gcgtcacgac gtcaacccgc gcggcaaata
    13921 tatgccaggt aaaccagcgt ggtatatgta cgatagctgc cagagcaaac gcagcgacag
    13981 tatcggtgtt ctctgctctg cggtcttctg gtcacaaaac aatggcctgc aactgcaaaa
    14041 tctgaccatc gaaaacacgc tgggcgatag cgtagatgca ggtaaccatc cggcggtggc
    14101 actgcgtact gatggtgacc aggtacagat taacaacgtt aacattctcg gtcgtcagaa
    14161 caccttcttt gtcaccaaca gcggtgtgca gaaccgtctg gaaaccaacc gtcagccgcg
    14221 tacgctggtg accaacagct acattgaagg ggatgtggat atcgtttctg gtcgcggcgc
    14281 agtggtgttc gataacaccg aattccgcgt ggtgaactca cgtactcagc aagaagcgta
    14341 tgtgtttgca ccggctacgc tgtccaacat ttactacggt ttcctcgccg taaacagccg
    14401 tttcaatgct ttcggtgatg gtgtggcgca actgggccgc tcgctggatg ttgatgccaa
    14461 taccaacggt caggtggtga tccgtgatag cgccatcaac gaaggtttta acacggctaa
    14521 accgtgggcc gatgcggtga tctctaatcg tccgtttgcg ggtaataccg gcagcgtaga
    14581 tgataacgac gaaatacagc gcaatctgaa tgacactaac tacaaccgca tgtgggaata
    14641 caataaccgc ggcgtgggta gtaaagtggt tgcagaggcg aagaagtaag agctattaac
    14701 tatttgccgg atgcggcgta aacgccttat ccggcctaca gattcgatgc gattcgtagg
    14761 tcggataaga tgcgcaagca tcgcatccga caataagtgc cgaatgcgac ctacattcac
    14821 atggcgcttt ttacatctga cggtttttat tgaagttaat caaactaccc gccttgataa
    14881 tctcgcgctc ttcagcagtc agactttcca tatagagcgt aatttccgtt accggcgcat
    14941 cttcatggat cacataacct ttaaacgtcg tacccggatt atccagcgcc gctttaatgc
    15001 cagggatgta aatgtaatcc cccacttcaa aggttggcac ttccgccatc tgcagcggta
    15061 acatccccca gttgatgacg ttagaacggt agcgtttagt cgcgtactct tcggcaatat
    15121 tcgccagacc gccaatcaca cgctggcagc tcgccgcctg ttcacgcgca gaaccatcgc
    15181 ctggtttcac cgcatatacc atgctgccaa tttcagtttg cagcggatca atatgctcct
    15241 gaccagcaat ctgcttaatg cgcgcaaaca cctctgtcag ctcgctgaca ttccccgcca
    15301 gacgctgatt ttccagctca gcagtcgctt tacttctgcc aacataaccg ggatcgcggc
    15361 gtgacagggt aaactccgcc agaccaatcg gatttgaacg ataagaagag gtttcaccgg
    15421 aaggaatcag ttcgtcggtg gtggtcactt cgtcgaggat cttcgagcac actttcagga
    15481 cgatattgtc agtcagcgca cccaattccg gccagtcttt aatgttcggt ccgtaaatca
    15541 gcggttgctg agttgcccct ttcacaaagc cctgataaac acggttttta tacggcgtta
    15601 catcgaaggc gtactccggc acgttgtccc agcaatcaag ttcgctggca gaggttaaat
    15661 agccaccgtt tgccgcagtc gcagcgatag aacgagcgtc catcaacgcc accgctgaca
    15721 tctgcccatt agctggctta gagccttcgc ggttcgggaa gttacgcgtg gtgtggcgaa
    15781 tacttaatcc gttgttgatt ggcgtatcgc ccgcgccaaa gcatgggccg cagaacgcgg
    15841 ttctgatgat tgcgcctgcg ccaatcaaat ctgctaccac accttttttg gcgagatcta
    15901 taaacaccgg ctgtgatgac gggtaaactg ccagcgagaa ggtgtcattg ccacaggatt
    15961 gaccgcgcag tgcattcgcc gccgcgatga cgttttcgta gttaccgcca gaacagcccg
    16021 cgataatccc ctgctgcact ttcaggcgac cattttccac tttatccagc agcgagagtt
    16081 tggctttacc gtgcgccacg cgttcggact caatttcaat ctcacgcaga atgtcggtca
    16141 ggttctggtt cagtgtgtcg atttcataca cgttgctcgg gtggaacggc agcgcaatca
    16201 ttggtttgat ggcgcttaaa tcaacgctga tgcagccatc gtagtacgcc atcggttgag
    16261 ggttaagctg gcagtaatcc tggccgcgac cgtgcagcgc cagccagtta tgcacttctt
    16321 catcagtttg ccagacggag cttaaacagg tggtttccat ggtcatcacg tcaacgctgt
    16381 tacggaaatc ggtagagagc gcgctaacgc ccggtccaac gaactccatg actttgtttt
    16441 tgacgtaacc gtttttgaac accgcgccaa tgatagccag cgccacatcc tgcggcccca
    16501 catacggcgc tggttttccg gtcagatgca ccgcaacaac tcccggatag tcgatatccc
    16561 aggtgtcatt aagcagctgt tttaccaact caccaccgcc ctcaccgact gccattgtcc
    16621 ctaatgcacc gtaacgggtg tggctgtctg acccgaggat cattttgccg ccgcctgcca
    16681 tcatctcacg catatattga tggatgaccg caatatgcgg aggcacaaaa ataccgccat
    16741 aacgctgagc tgccgataaa ccaaaaacat ggtcatcacc gttaatggtg ccgccgacgg
    16801 cgcagagtga gttatggcag ttggtcagca catacggcag cgggaaacgt tccataccgg
    16861 acgctttagc ggtctgtaca ataccaacaa aggtaatatc gtgagaggct aatgaatcaa
    16921 acttaatttt aagtttatcc atatttccgg acgtattatg cgaagagaga atagaccagg
    16981 caatagtgcc ttttttggct tcttcttttt taatttcgcc ggtgaaatgt tcttcagcaa
    17041 ttatttcgtt attactggcg agaaacacgc ctttttcaga taacttgatc atcaaatgct
    17101 ccagatatct gcgcaccgtg aagtgcgcag aaagataata aatggatata ctctaaataa
    17161 tttgagttgc aggaaggcga caagcgagtg aatccccagg agcttacata agtaagtgac
    17221 tggggtgagc gaacgcagac gcagcacatg caacttgaag tatgacgaga atatttacag
    17281 catgttccag ccgatcagca tattccacca ccacacgccg agggtgatat gcaccaggaa
    17341 ggttaatatc gtcagtaccg caccgaccaa ccaccaggat ttaatatcgt tataacccac
    17401 gccaaagatg accggacctg ccgcgccgcc atagtgagta accatgccgc cataggagtt
    17461 ggagaacaac agtgccagcg cggttaacat taacggtgcg ccggagacgt tcgccagcat
    17521 ggcaaatacc ggtaacatag caacgatata ggcactaccg gaagcgaaga aataacgcac
    17581 gataatgctg aggaaaataa taacgaagaa agcaacgtta ccgtgaccat caaatgccag
    17641 gttattttta aagacttcag ctaaccattc gaagaattta actttcgata ataaggagct
    17701 taatccgata ataccgccgt accagattaa ggtattccag ccgcctttat ttttaaccac
    17761 gtcttcccag gtaacgatac ccagcagcag catggtcgcc ataacaacga ttgccacagt
    17821 ggattcatca acccccagag acttactgaa aatccagccc agtagcgcca gcacaaagac
    17881 accgagcagc attttttcgc ggattttcat cggtcccagt tcggcaaggc ccgctttagc
    17941 gatggttttg ttatccacct ttttaatttc tggtggatac atggtgtaaa ttaccagcgg
    18001 ggtgaccagc agcataatga tgcccggcaa tccggcggcc agcgcccatc caccccagct
    18061 aatttgcagg tgcagaatgt cgttgatcat tttcagcgcc agaatgttcc ccgccattgc
    18121 ggtaaagaac atatagctgg tggttttggt gaccatgtaa atggacatca tcaggtaatg
    18181 tccgacacga cgcggacttt tttccggttc ggaccccaaa gccaccgcca cgctgttgat
    18241 gatcggtaac acaatgccgc ccgcacgcgc ggtgttagac ggtgttgccg gagccagtac
    18301 cagatcgagg aataccgtaa cgtaacccag acccagcgtg gtgttaccga ttttaccaat
    18361 cagcagatag gcaatacgtt tacctaaacc ggtggtcaca aatgcggcgc ttaaggtaaa
    18421 cgccgagaac accagccagg tagtacctga agagtaaccg cttaatacgg cggtggtctt
    18481 aaacgcaccg tcggataagt taccgaccac caccattgag gcagcaacgg caattaacag
    18541 tacgacaggt tccgggaaag gcttgataac cagacccaca atggccgcca ggtaaatacc
    18601 aaaaagcacc cacgccagtt cgcttaatcc tgccggagct ggcataaaac caataataca
    18661 tgggatcgct aatatcagaa ttagcttcca taacgatttc ttattcattt aaaatcctgt
    18721 tctttctgta tgactcatac agaagtcctt tattgattgt ccttatcctg acaacgaatt
    18781 ttttcaggga agataaactt caccggaaaa tatttttctg gtcgtccgaa taacagatgc
    18841 gcgtaacgtc gtggcatcct gaccttcatt acttaaatga acgtcgagcg caccactggg
    18901 gtgttcaata ttgatattgc cgtatcctac agaagggacg atttgtcggg tgacggtgcc
    18961 ttccaatgca caactactgg aaatagcaat agcaccggtt atcgccagcg cgcgatggca
    19021 agaatgcggc ataaaataac gtacattaat tgccccgcct ttctgcgcta gagaaataag
    19081 cacaggttta gggataacca tattactgac atcacctaag cccattgctt tacccgcttg
    19141 tagacggata gattcaatgc gtgctaataa tgctttgtcg gcatccagtt ccgccggtaa
    19201 ttcataacct gtttttccca gatattcagc cggaataatg acgactggca tcgccatatc
    19261 gatacaggtc accgggacat cgtcaaaata atcaatctga ttatcagtcg ggaagacttt
    19321 tccggttttg gttccggcgg cattcaggaa agtgagcgca accggtgcgg cagtacccgg
    19381 tacgccgtca attctggcgc taccctcgta ctcgacaaca ccatttggcg tttgcacatc
    19441 agcttcgatg aacgtaccag tattgacgtt gcggatacgt acgcgggtaa ctggtgaagt
    19501 cgctgcaatc aaaccatttt caatggcgaa tgccccaaca ccagacagca tattgccaca
    19561 attaggcgtg gtatcgacac gttgctcatg gacaataact tgagcaaaca gataatcgac
    19621 atcagcacgc ggatcgctgg aacggctaat aatggcgact ttactggtca gcggattacc
    19681 gccgccaata ccgtcaattt ccagatcgtt accggaaccc ataattgcca tcaatatttt
    19741 atcgcgctgc gtttgatctt cgggtaaatg ttccgctaac aagaacgcgc ccctcgaggt
    19801 tccacctcgc atcatcacgc agggt
//
LOCUS       DAZGXZ010000095        19622 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000095 DAZGXZ010000000
VERSION     DAZGXZ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.095"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..289
                     /locus_tag="ACHKJF_003518"
     CDS             <1..289
                     /locus_tag="ACHKJF_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950820.1"
                     /translation="SEKIYDYIDIIIEDYENIKENAYLVIPQINYTMDLNIEDSSSEE
                     FLFDNGLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEYTYDYDLLNAI"
     gene            complement(396..772)
                     /gene="rnpB"
                     /locus_tag="ACHKJF_003519"
     ncRNA           complement(396..772)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKJF_003519"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(805..1950)
                     /gene="garK"
                     /locus_tag="ACHKJF_003520"
     CDS             complement(805..1950)
                     /gene="garK"
                     /locus_tag="ACHKJF_003520"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP0950821.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISDLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2047..2937)
                     /gene="garR"
                     /locus_tag="ACHKJF_003521"
     CDS             complement(2047..2937)
                     /gene="garR"
                     /locus_tag="ACHKJF_003521"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0950822.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2967..3737)
                     /gene="garL"
                     /locus_tag="ACHKJF_003522"
     CDS             complement(2967..3737)
                     /gene="garL"
                     /locus_tag="ACHKJF_003522"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP0950823.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     IEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3753..5087)
                     /gene="garP"
                     /locus_tag="ACHKJF_003523"
     CDS             complement(3753..5087)
                     /gene="garP"
                     /locus_tag="ACHKJF_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP0950824.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5462..7033
                     /gene="garD"
                     /locus_tag="ACHKJF_003524"
     CDS             5462..7033
                     /gene="garD"
                     /locus_tag="ACHKJF_003524"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP0950825.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQDIPLENASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNDEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            7182..7517
                     /gene="prlF"
                     /locus_tag="ACHKJF_003525"
     CDS             7182..7517
                     /gene="prlF"
                     /locus_tag="ACHKJF_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP0950826.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7517..7981
                     /gene="yhaV"
                     /locus_tag="ACHKJF_003526"
     CDS             7517..7981
                     /gene="yhaV"
                     /locus_tag="ACHKJF_003526"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP0950827.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(8036..8845)
                     /gene="agaR"
                     /locus_tag="ACHKJF_003527"
     CDS             complement(8036..8845)
                     /gene="agaR"
                     /locus_tag="ACHKJF_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP0950828.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            9094..10374
                     /gene="kbaZ"
                     /locus_tag="ACHKJF_003528"
     CDS             9094..10374
                     /gene="kbaZ"
                     /locus_tag="ACHKJF_003528"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP0950829.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            10397..10870
                     /gene="agaV"
                     /locus_tag="ACHKJF_003529"
     CDS             10397..10870
                     /gene="agaV"
                     /locus_tag="ACHKJF_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP0950830.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10881..11660
                     /gene="agaW"
                     /locus_tag="ACHKJF_003530"
     CDS             10881..11660
                     /gene="agaW"
                     /locus_tag="ACHKJF_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP0950831.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11650..12528
                     /gene="agaE"
                     /locus_tag="ACHKJF_003531"
     CDS             11650..12528
                     /gene="agaE"
                     /locus_tag="ACHKJF_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP0950832.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12546..12980
                     /gene="agaF"
                     /locus_tag="ACHKJF_003532"
     CDS             12546..12980
                     /gene="agaF"
                     /locus_tag="ACHKJF_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP0950833.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12977..14110
                     /gene="nagA"
                     /locus_tag="ACHKJF_003533"
     CDS             12977..14110
                     /gene="nagA"
                     /locus_tag="ACHKJF_003533"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0950834.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            14460..15614
                     /locus_tag="ACHKJF_003534"
     CDS             14460..15614
                     /locus_tag="ACHKJF_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP0950835.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15627..16487
                     /gene="kbaY"
                     /locus_tag="ACHKJF_003535"
     CDS             15627..16487
                     /gene="kbaY"
                     /locus_tag="ACHKJF_003535"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP0950836.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            16654..17130
                     /gene="agaB"
                     /locus_tag="ACHKJF_003536"
     CDS             16654..17130
                     /gene="agaB"
                     /locus_tag="ACHKJF_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP0950837.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            17169..17972
                     /gene="agaC"
                     /locus_tag="ACHKJF_003537"
     CDS             17169..17972
                     /gene="agaC"
                     /locus_tag="ACHKJF_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP0950838.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            17962..18753
                     /gene="agaD"
                     /locus_tag="ACHKJF_003538"
     CDS             17962..18753
                     /gene="agaD"
                     /locus_tag="ACHKJF_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP0950839.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            18754..19509
                     /locus_tag="ACHKJF_003539"
     CDS             18754..19509
                     /locus_tag="ACHKJF_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP0950840.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         4803 a         5078 c         4976 g         4765 t
ORIGIN      
        1 atcagaaaaa atatatgatt acatcgatat aatcattgaa gattatgaaa atatcaaaga
       61 aaatgcttat ctggtcattc cccaaattaa ttatactatg gatttaaaca tagaagactc
      121 tagctcagag gaattccttt ttgataatgg cctcgagaaa gacgaaaatt cttcggacaa
      181 ttgctttgaa gtcgaaaaat ataacgcata tgaagcatat aactcagaga atctatattt
      241 taccagagag gaatatacgt atgattacga ccttttaaat gcaatataga cttaataact
      301 tcttacaaaa tctataatta ataccgtaga tattcattca tcttaccctg tgcaccaaaa
      361 gcaaaaaccc gccgaagcgg gtttttacgt aaaacaggtg aaactgaccg ataagccggg
      421 ttctgtcgtg gacagtcatt catctaggcc agcaatcgct cactggctca agcagcctac
      481 ccgggttcag tacgggccgt accttatgaa cccctatttg gccttgctcc gggtggagtt
      541 taccgtgcca cggactgtta ccagccgcgc ggtgcgctct taccgcaccc tttcaccctt
      601 acctgatccc gcttgcgcgg gccatcggcg gtttgctctc tgttgcactg gtcgtgggtt
      661 tcccccccag gcgttacctg gcaccctgcc ctatggagcc cggactttcc tcccctccgc
      721 ccgtctcccc cgaagaggac gacgacgaag cggcgactgt ctggtcagct tcggcgcgca
      781 gtatagaggg tttgcgcgcc cttgtcaccc cgcgttgcgc attccaatcg ccagtgtcgc
      841 ggcaatatta cgtgaagcac ggcagatatt gtcataagcc ccgcggaatg cttcgtccaa
      901 cgtacctatg ctggtcaata cgctgaagac cgcatcaatg ccatgctgat gtacaacgcc
      961 aacatcatcg gtcaggctac ccgcaatgcc aatcaccggt ttatggtact tcttcgccac
     1021 gtttgcgacg ccaatcggta ccttgccgtg aatactctgg ctgtcaatac gcccttcacc
     1081 ggtgatcacc agcgtacaat cgtgaatatg ttcctccaga ttcagcgccg tagtgacgat
     1141 ttcaatacca cttttcagtt ccgcaccaag aaacgccatc agcgccgcgc ccataccgcc
     1201 agcagctcct gcgccgggga catctttcac atcaacatgc agcgcttttt taatgacatc
     1261 ggcatagtga gagaggttat tgtccagctc aacaatcatc gcttcactgg ctcccttttg
     1321 tgggccaaag atgcgcgatg cgccgttatc gcccaccagc ggattggtga catcacaagc
     1381 gacgcgaatg acgcaatcct ttaagcgcgg atcgaggtcg gaaatatcaa tatcattcag
     1441 agtattaaga ctaccgccgc caaaaccaat ttcattgccg ttggcgtcgc ataatttcgc
     1501 ccccagcgcc tgtaccatgc ctgcgccgcc atcatttgta gcgctgccgc caatgccgat
     1561 aataatgttt gtcgcgccgc tctccagcgc ctgcaggatt aactcgcctg tgccgcgtga
     1621 agtggtcacg agtggatcgc gtttttccgc aggtaccagc tccagcccac tggccgccgc
     1681 catttcaata aacgcggttt tgccatcgcc ggagatcccc caactggcat tcactttctc
     1741 gcccagcggc cctgtaaccc aggcgtgacg ttcagccccc tgggtggctg caatcatcgc
     1801 ttccaccgtt ccttcgccac cgtcggcaac aggaacagaa acgtactgtg catcaggaaa
     1861 aatttcccga aatccttttt ctatcgcctg cgcaacctcg ctggcagata aactttcttt
     1921 ataagagtct ggggcgatta cgattttcat acctatgcct gttaccacat gacgccggag
     1981 ggcgtttctc ttattcggcc tggattccag gcccggattg caatacgcca tccgggcacg
     2041 acgtcattaa cgagtaactt cgactttcgc cagtttttcg tagtagcacg ccagggcgct
     2101 atgatccgcc gttcctaaac catctgcacg cagtgcctgc atcatctcca taaccgcagc
     2161 tgtgagcggc agttgtgcgc cgacgccgtg ggaagtatcc agcgcatttg ccagatcctt
     2221 aatatgcaga tcaatacgga agcccggctt gaagttgcgg tccatcacca tcggcgcttt
     2281 ggcatccagc acggtactgc ccgccagtcc accgcgaatt gcctgataca ccaggtccgg
     2341 gttaacgccc gctttggttg ccagcgttag cgcttctgac atcgcggcaa tattcagcgc
     2401 cacaatgacc tgatttgcca gtttggtgac gttacctgca ccgatttccc cggtatgcac
     2461 cacggaaccc gccatcgctt tcatcaaatc atagtatttg tcgaaaatag ccttgtcgcc
     2521 gcccaccatc actgacagcg taccgtcgat ggctttcggt tcaccgccgc tcaccggagc
     2581 atccagcata tcaatgcctt tcgctttcag cgcttcgctg atttcacggc ttgccagcgg
     2641 tgcgatagaa ctcatatcga tcaataccgt acctggcttc gcgccttcaa tgatgccatt
     2701 ctcacccagc gccacctctt tcacatgagg ggagtttggc agcatggtta tgatgacgtc
     2761 gcactgttca gcgatcgctt tagccgtaga cgctgtttct gcacctgcag caatcacgtc
     2821 agcaatagct tctgggttac ggtcagcaac caccagcgag taacctgctt tcagaaggtt
     2881 tttactcatt ggtttaccca taatccccag gccaataaaa ccaactttca tagtcatatc
     2941 aatcatctct cttgttgcgg tggtggttat tttttaaagg tatcagccag tttctgagtg
     3001 gcagagcgga agacgccgag atcgctgccg acagccacaa acgtcgcgcc ccattccaga
     3061 taacgacgcg catcggcttc gatcggtgcg aggataccgc tgggtttgcc gtgcgcactg
     3121 gcacggttaa aaatgtgctg aattgctttt tgtacatccg ggtgcgaggc attgccgaga
     3181 tggcctaatg ccgcagccag atcgctgggg ccgacgaaga tgccgtctac gccttcggta
     3241 gcggcaatgg catcgacatt atctacgccc tgctgacttt ctatctggac cagaatagtg
     3301 atgttcttgt tcgactgagc gaaataatcc gccacggtgc caaacatatt ggcgcggtga
     3361 gaaacggaga cgccgcgaat gccttccggc gggtaacgag ttgatgccac cgcctgctct
     3421 gcttcctctt ttgtttctac aaaaggaatc aggaagttat agaaaccgat atccagaaga
     3481 cgcttaataa ttaccggctc gttggtcggc actcgcacta ctggcgcgct ggcgctgcct
     3541 ttcagggcca ttaactgcgg aataaacgtg gaaatatcgt ttggcgcatg ttcgccatcc
     3601 agcaccagcc agtcaaaccc agccaaacca agaacttcag tgctaatcgg gttagagagt
     3661 gctgaccagc aaccaatttg tacctgtttc gcagccagtg cggctttgaa tttattcggg
     3721 aaaacatcgt tattcatcgc ttataccttt gcttatttct gcaattccat acgtttaatg
     3781 tcgccaacta cgaagaggta gcagaccatc gccatcagcg ctgaacatcc cacgaaaacc
     3841 agtgctgcat tgaaggagtg cagttcactt accaggtagc caatcaccag tggagtgaca
     3901 atggaggcaa cattgccaaa gacgttaaag acgccgccac agaggccaac aatctctttc
     3961 ggcgcggtgt cagaaatcac cggccagccc agagcaccaa atcctttgcc aaaaaaagcc
     4021 agcgccatca gcatgaccac cagcgtggtg ttgttggtgt agttacataa gatgatggtg
     4081 gaagccagca acattcccag cacaatcggt agcttacgtg ccagggtcag ggataaaccg
     4141 cgtttgatca gataatccga gaagacacct cccagcacgc cgcccgcaaa accacacagt
     4201 gccggaatcg aggcgaccag acccactttc agaatcgaca tgcctttttc ctgcaccaga
     4261 taaatcggga accaagtgag gaagaaccag gtgatggtgt tgataaaata ttgtccgaaa
     4321 aatacgccca acatcatgcg gttagagagc aattgcttga tgtaatgcag tttgggaccg
     4381 cttgctgccg cgctgcccgg ctttttgtgg tccatatcga ccaccgcgcc attttcagag
     4441 ataaacttca gctcttccgc agacatacgt gggtgatctg tcgggttatg aatcaacttg
     4501 atccacagcg ccgtcagcac aaaaccaatc ccccccataa cggtgaagac gtgctcccag
     4561 ccccaggcga aagtcagcca gccaagcagc ggcgaaaaga gcgccagcga gaaatattgc
     4621 gccgagttaa agatggcgga ggcagtacca cgttcttttg tcggaaacca ggcggcgaca
     4681 atacgggcgt tcgccgggaa tgatggcgct tccgagaacc ccagcataaa gcgcataaag
     4741 aacatggaga tccctgccca ggccagcggg aacatatcaa caaagccttg caggaaggtg
     4801 aacagcgacc agaaaaagag gctgtaggtg taaacttttt tcgagccaaa cttatcaagc
     4861 agccagccgc cgggaatttg catcagcagg taggcccagc caaaagcgga gaagatgtaa
     4921 cccatcgaaa ccgcacttaa ctgcaactct tttgccactt cggtaccagc aatagacagc
     4981 gttgcacgat cggcgtagtt aacggcggta acaataaaaa taatcagtaa tattaaatag
     5041 cgggtatgca cgcctttctt ttttacgtca acggtgtcca gaatcatttt atttacctcg
     5101 ggtacttatg ctgattttta ttattatggt gaaggtgtta tttatgagtt ttatttatgc
     5161 cgttacgaca atgaactcgg gaattagtat aagcagcgcg agaataataa tcattgtgca
     5221 aatgctaatt taattaatac tatttaaata ttattttgag catatgcaca taaggttgcg
     5281 cgctaaagca cagatttgcg ctttaccttg ccgggcggca ctgcaatccc tgaaatgatt
     5341 gacattgatc acatttctgc gtttaaactc ctgacattct tatttcaccc aatgaagtca
     5401 tttattttta aatgagacca ggtcctcatt ttaataaccc ctggctggag aatattgcac
     5461 aatggccaac atcgaaatca gacaagaaac gccaactgcg ttttatataa aagttcacga
     5521 cacagataat gtggcaatta ttgttaatga taatggcttg aaagcaggaa cgcgttttcc
     5581 ggatgggctg gaattaattg aacatattcc ccaggggcat aaagtcgcat tgctggacat
     5641 tccggctaat ggtgaaatta ttcgttatgg cgaagtgatt ggttacgccg tgcgcgcaat
     5701 cccacgcgga agctggatcg atgaatcaat ggttgtacta ccggaagcgc cgccgttaca
     5761 cacgctgcca ctggcaacca aagtgccgga acccttaccg ccgcttgaag gttacacctt
     5821 tgaaggctat cgcaatgccg atggcagcgt gggcaccaaa aacctgctcg gcatcaccac
     5881 cagcgtccac tgtgtggcag gcgtggtgga ctacgtagta aaaatcattg aacgcgatct
     5941 gctgccgaaa tacccgaacg tcgatggcgt ggtggggctg aatcatctgt acggttgtgg
     6001 cgtggcgatt aacgctccgg cggcggtggt gcccattcgg accattcaca acatttcgct
     6061 taacccaaac tttggcggcg aggtgatggt gattggcctg ggttgcgaga aattacagcc
     6121 agaacgcctg ctggtcggca cggatgatgt acaagatatt ccgctggaga acgccagtat
     6181 tgtcagcttg caggatgaaa agcatgtcgg ttttcagtcc atggtcgagg atattttgca
     6241 gatcgccgaa cgccatctac aaaaactgaa tcaacggcag cgagaaacct gcccggcttc
     6301 agaactggtc gttggtatgc agtgcggtgg cagcgatgcg ttttctggtg taacggcaaa
     6361 cccggcggtt ggctatgcgt ctgatctact ggtgcgctgc ggcgcaacgg tgatgttttc
     6421 agaagtaacg gaagtgcgtg acgcgatcca tctgctgaca ccacgcgcag tgaacgacga
     6481 ggtcggcaaa cggctgctgg aggagatgga gtggtacgat aactatctca atatgggaaa
     6541 aaccgaccgc agcgccaacc cttcgccggg caacaagaaa ggcggtctgg caaatgtggt
     6601 ggagaaggca ctcggctcca ttgctaaatc gggtaaaagt gcaattgttg aagtgctgtc
     6661 gcccggtcaa cgcccgacta aacgcggatt aatttacgcc gcgacgccag ccagcgattt
     6721 tgtctgtggc acgcaacagg tggcttcggg tatcaccgta caagtgttta cgaccggccg
     6781 tggtacgccg tacggcctga tggcggtacc cgtcattaaa atggcaaccc gcaccgagct
     6841 ggcgaaccgc tggtttgatt taatggatat taacgcgggc actatcgcta ccggcgaaga
     6901 gagcattgaa gaggtgggct ggaagttgtt ccactttatt ctcgacgtcg ccagcgggaa
     6961 gaagaaaacc ttctcggatc aatgggggct gcataaccag ctggcggtgt ttaacccggc
     7021 accggtgacc tgatttcttt tggaacgtcc ctcgcaaaac atggccttag tgccatgttt
     7081 ttattgttta aagcccccac gtccattaat aatgcatttg cattacctta gttcaagctt
     7141 ataatttgag cagaaaacag gctgtaaaag gacagtgaat catgcccgct aatgctcgct
     7201 ctcacgctgt actgaccact gaatcaaagg tcacgatacg cggacaaaca actatccccg
     7261 cgccagtgcg tgaggcctta aaactgaagc caggccagga cagcattcat tacgaaattc
     7321 tgcctggtgg gcaagtattt atgtgccgac tgggagatga acaggaggat catactatga
     7381 atgcattttt gcgttttctg gatacagata tccagaacaa cccgcaaaaa actcgtccat
     7441 tcaacattca acaaggaaag aaacttgtcg ctggcatgga cgtcaacatt gatgatgaga
     7501 ttggcgacga cgaataatgg attttccaca aagggttaat ggttgggcgc tatatgctca
     7561 tccctgtttt caggaaacct acgacgcttt agttgccgaa gtcgagacat taaagggaaa
     7621 agatcctgaa aattatcaga gaaaagccgc cacaaagtta ttggcggtag tccataaagt
     7681 gattgaggag catatcacgg tcaatccatc atcaccggca ttccgtcatg gcaagtcgtt
     7741 aggctctggg aaaaataaag actggtcacg ggtaaaattt ggtgctggtc gttatcgtct
     7801 cttctttcgt tatagtgaaa aagagaaagt catcattctg ggatggatga acgatgaaaa
     7861 cactctgcgc acctacggta aaaaaacaga tgcctatacc gtattcagca aaatgttaaa
     7921 aagaggacat cctcctgccg actgggaaag cctcacccaa gagacagaag aaaaccattg
     7981 atggtgttca caatgcccgc cgaacgtatt cgtgttgacc agcgggcacc ctttctcact
     8041 ccccgaccag aatcacttca accccagcct ttcgcagtcc ttccagacta tccgcaggaa
     8101 tgccttcatc aacaatgatc atgtcgatac gttgagtatc aatgatctta tgtaaactgg
     8161 aacgattgaa tttactggaa tcggtgacca cgatgatccg ttccgcaact tcgcacatcc
     8221 gacggtttaa acgggcttca tcttcattat gtgtgctgac gccgcgctcc agatcgatcg
     8281 catctacacc aagaaacagc atatcgaagt ggtaattttg cagcgattgc tcagcctgat
     8341 cgccgtaaaa agattgcgac tgacggcgca aatgcccgcc ggtcatcagc agctcaacgc
     8401 cttccgcttc cagcaacgca ttagccacgt tcataccgtt ggtcatcgca attacgtcag
     8461 tgtgcttacg catcagacga gcaatctcaa aagtggtagt cccggaatcg aggatcaccc
     8521 gatgaccagg ctgaatcaac tcaacggcag ctttcgcaac gctgcgtttc atcgcggtgt
     8581 tcagcgcgct tttatcttcc actgatggct cgactgacgg cgtcgtgcta tcgcagatca
     8641 acgcgccacc ataggcacgc acagcgatcc cctgcttttc cagaaacgcc agatcattgc
     8701 ggatcgtcac agtagatacg ccatacaatg ccgacagatc gttaacctgc acactccctt
     8761 gctgtcgcag acgctgaatg atctgttctc gtcgctcgct ggtgccagtc actcgcttct
     8821 cacctgaagc gtcggtatta ctcatagtaa gtcctttcgt aaaactttcg tttcatttcg
     8881 ttttgcctat taacgccttt ctattaagca aatgcaagcc caccttgccc attggcgcaa
     8941 gctactctcg tttcagtgac tttcattatg tttcttttgt gaatcagatc agaaaactat
     9001 tatctttcgt tttattttta tctcaccatg acgcagtatc aactgaaaca aaacgaaaga
     9061 ttaatatcgc agtaatctga actggagagg aaagtgaaac atctgacaga aatggtgaga
     9121 cagcacaaag cgggcaaaac aaatggaatt tatgccgttt gttccgcaca tccgctggtg
     9181 ctggaggctg caatccgcta cgccagtgca aaccaaacgc cgttactgat tgaagcaacc
     9241 tccaatcagg tagaccagtt cggcggttat accggaatga cgcccgccga ttttcgcggc
     9301 tttgtttgtc agctcgccga ctcgttgaat ttcccgcagg atgcgttgat tctgggtggt
     9361 gaccatctgg ggccaaaccg ctggcaaaac ctgccggccg ctcaggcaat ggccaatgcc
     9421 gatgatttga ttaaaagcta cgttgcggca ggattcaaaa aaatccacct tgattgcagc
     9481 atgtcctgtc aggacgatcc gattccctta actgatgaca tcgtggctga acgcgccgcc
     9541 cgtctggcga aagtggcgga agaaacctgt cttgaacact ttggcgaagc cgatctggag
     9601 tatgtcattg gtaccgaagt gccggtacct ggcggcgcgc atgaaacctt aagcgagctg
     9661 gcggtcacca cgccggatgc cgcccgcgcc acgctggaag cccatcgtca cgcctttgaa
     9721 aagcaaggtt tgaatgccat ctggccacgc atcattgccc tggtggttca acccggcgtc
     9781 gaattcgatc acaccaacgt tattgattat cagcccgcca aagcgagcgc cttaagccag
     9841 atggtcgaaa actacgaaac gctgattttc gaagcgcact ctaccgatta tcaaacgccg
     9901 caatcgctgc gccagctggt gattgaccac tttgccattc tgaaagttgg cccagcgctg
     9961 accttcgccc tgcgtgaagc tctgttctct ctggcggcga ttgaagaaga actggtgcca
    10021 gcgaaagcct gttctggtct gcgtcaggtg ctggaagacg tgatgctcga ccgcccggaa
    10081 tactggcaaa gccactacca cggtgacggc aacgcgcgtc gtctggcgcg tggttatagc
    10141 tactcggatc gcgtgcgcta ttactggccg gacagccaga ttgatgacgc tttcgctcat
    10201 ctggttcgta atctggcgga ttcaccaatt ccgctaccgc tcatcagcca gtatctgccg
    10261 ctgcaatacg tgaaagttcg ctccggcgag ctgcaaccga cgccacggga actcattatc
    10321 aaccatattc aggacatcct ggcgcagtac tacacagcct gtgaaggcca ataagcaaaa
    10381 caaagaggaa cacgctatgc caaatattgt tttaagccgg attgatgaac gcttgattca
    10441 cggtcaggtc ggcgttcagt gggtcggatt tgcgggggca aatctggtgc tggtagccaa
    10501 cgatgaggtt gccgaagatc cggtacaaca aaacctgatg gaaatggtac tggcagaagg
    10561 gatcgccgta cgtttctgga cgctgcaaaa agttatcgac aacattcatc gcgccgccga
    10621 tcgacagaaa atcctgctgg tttgtaaaac acccgccgat ttcctgacgc tggtgaaagg
    10681 tggcgttccg gtgaatcgca ttaacgttgg caatatgcac tacgccaatg gcaaacaaca
    10741 aatcgccaaa acggtttctg tggatgcggg cgatatcgca gcatttaacg acctgaaagc
    10801 cgctggggtg gaatgcttcg ttcagggcgt cccgacagag cctgctgtgg acctctttaa
    10861 attactttga gggattcatc atggaaatca gcctgttgca ggcatttgcg ttgggcatta
    10921 tcgcctttat cgctggcctg gatatgttta acggcctaac acatatgcac cgcccggtgg
    10981 tcctcggccc gttggtcggg ctggtacttg gcgatctgca taccggaatt ttaaccggcg
    11041 gtacgctgga actggtgtgg atggggctgg ccccgctggc gggcgcacag ccgcctaacg
    11101 tgattatcgg tactatcgtc ggcacggcgt ttgccattac tactggcgtg aaacccgatg
    11161 tcgcggtcgg tgtcgccgta cctttcgctg tcgcagtaca gatggggatt accttcctgt
    11221 tctcggtgat gtctggcgtg atgtctcgct gcgaccggat ggcggagaac gccgatactc
    11281 gcggcattga acgcgtgaac tatctggcac tgctggcact cggcactttc tattttctct
    11341 gcgctttcct gcctatctac ttcggcgcgg aacatgcaaa aaccatcatt gatgtcctgc
    11401 cgcaacgatt aatcgacggc ctcggcgtcg cgggcggcat catgccagca atcggttttg
    11461 ccgtgctgct gaaaatcatg atgaaaaacg tctacatccc ctacttcatc ctgggctttg
    11521 ttgccgcagc ctggctcaag ttaccggtgc tggctattgc tgccgctgcg ctggcgatgg
    11581 cgctgatcga cctgctgcgt aaatctcctg aaccgactca acctgcggca cagaaagagg
    11641 aatttgaaga tggcatctaa tcaaaccacc ctgccgaacg tcagtgaaaa cgaagaaaca
    11701 ttgctgactg gcgtcaatga aaacgtgtat gaagatcaga gcattggcgc ggagctgacg
    11761 aaaaaagata tcaaccgcgt cgcctggcgt tccatgctgt tacaagcgtc ttttaactac
    11821 gaacgtatgc aggcttctgg ctggttgtac ggtctgctgc ctgctctgaa aaagatccac
    11881 actaataaac gcgacctggc gcgcgccatg aaggggcata tgggtttctt caatacccat
    11941 ccgtttctgg tgacatttgt tatcggcatt atccttgcga tggagcgttc taagcaggac
    12001 gttaacagta ttcagagcac caaaattgcc gtcggtgcgc cgctcggcgg gattggcgat
    12061 gcaatgttct ggctaacgct actgccgatt tgtggcggga taggcgcaag ccttgcgcta
    12121 caaggctcta ttcttggcgc agtcgtattt attgtgctgt tcaacgtggt acacctgggg
    12181 ctgcgttttg gtctggcgca ttatgcttac cgcatgggcg tggcggcgat tccactcatt
    12241 aaagcgaata ccaaaaaagt cggccatgct gcgtctatcg ttgggatgac ggtaatcggc
    12301 gcgctggtgg caacctatgt tcgtttaagc accacgctgg aaatcacagc gggcgacgca
    12361 gtggttaagt tacagactga tgttattgac aaactgatgc ctgccttctt accgctggtc
    12421 tacaccttga ccatgttctg gctggtacgc cgcggctgga gtccgctgcg tctgattgca
    12481 gttaccgtgg ttctcggcat cgtcggtaaa ttctgccatt tcctttaaat acaatgaggt
    12541 attagatgtt aagtattatt ttgacagggc atggcgggtt tgccagcggc atggaaaaag
    12601 caatgaagca aattctcggt gagcaatcgc agtttatcgc catcgatttt ccggaaacct
    12661 caagcaccgc gctgcttaca tcgcagcttg aagaggccat cgcccaactg gattgtgaag
    12721 acggcattgt ttttctgacg gatttgctgg gcggcacgcc gtttcgcgta gcttcaacgc
    12781 tggcgatgca aaaaccgggc tgtgaagtga tcaccggcac caatttgcaa ctgctactgg
    12841 agatggtgct ggagcgcgaa gggttaagcg gtgaagaatt ccgtgtacag gcgctggagt
    12901 gtggacaccg agggctgacc agtctggtgg acgagttagg ccgctgccat gaagaatgtc
    12961 cggtcgagga aggaatatga cacacgttct gcgcgccaga aggctgctga ctgaagaggg
    13021 atggctcgat gaccatcagt tgcgtattgc tgacggtgtc atcgcagcaa tcgaaccgat
    13081 tccagtgggc gtgactgaac gcgatgcgga actgctctgc cccgcttaca tcgacaccca
    13141 tgtacacggt ggtgcgggcg ttgatgtaat ggatgacgcg ccggatgtac tcgacaagct
    13201 ggcaatgcac aaggcacgcg aaggtgtcgg cagttggttg ccgactaccg taaccgcgcc
    13261 gcttaacagc attcatgcgg cgctgaaacg tattgctcaa cgttgccaac gcggcggacc
    13321 tggtgcgcaa gtgctgggga gttatctcga aggaccgtac ttcacgccgc agaataaagg
    13381 cgcgcatccg ccggaattgt ttcgcgagct tgaaattgcc gagctggatc aattgattgc
    13441 cgtttctcag cacaccttac gcgtggtagc gctggcaccg gaaaaagagg gggcattgca
    13501 ggccatccgc catcttaaac agcaaaatgt acgagtgatg ctggggcata gcgcggcgac
    13561 ctggcaacaa actcgcgccg cgtttgatgc tggtgccgac ggcctggtgc attgctataa
    13621 cgggatgaca ggtttacatc accgcgaacc gggaatggtt ggcgcgggat taacggacaa
    13681 gcgcgcctgg ctggaactga tagccgatgg tcatcatgtg catccggcgg cgatgtcgct
    13741 gtgttgttgc tgtgcaaaag agagaatcgt gctgatcacc gacgcgatgc aggcagccgg
    13801 gatgccggat ggtcgctata cgttatgtgg cgaagaagtg cagatgcacg gtggcgttgt
    13861 ccgtaccgcg tccggtgggc tggcgggcag tacgctgtct gttgatgcgg cagtgcgcaa
    13921 catggtcgag ttgacgggcg taacgcctgc ggaagccatt catatggcat cgctgcatcc
    13981 ggcgcgaatg ctgggtgttg atggtgttct gggatcgctt aaaccgggca aacgcgccag
    14041 cgtcgttgcg ctggatagcg ggctacatgt gcaacaaatc tggattcagg gtcaattagc
    14101 ttcgttttga tggtttgctc cattattggg ccttcacttc ccccgtaagg cctttctttt
    14161 tctttctttt tgatctgtgc agcggtgtcg gatgcgacgc taacgcgtct tatgcgacct
    14221 acggtacgcc gcatccggca atcggtgaac atagcgacgt cattacgcaa ttcttaatct
    14281 tcccttgtct tttctttcaa cgatcacaga tttcgtttta tttccttttt ctccattgaa
    14341 ctttcagttt cttttctata gattttaatc aacgaaagac atcaccaagt gaaatgaaac
    14401 gaaaggcaag tgaaagcgac aacgcccgac gtcaagttca tcagactaag gattgagtta
    14461 tgccagaaaa ttacacccct gctgccgccg caaccggtac atggactgaa gaagagatcc
    14521 gccatcagcc tcgcgcatgg atccgttcac tcaccaacat cgacgcgcta cgttccgcgc
    14581 tcaataactt ccttgaaccg ttactgcgca aagagaatct gcggatcatc ctgaccggag
    14641 ccggaacgtc ggcatttatc ggtgacatca tcgcgccgtg gctcgccagc cataccggta
    14701 aaaacttcag cgccgtaccg accaccgatc tggtcaccaa tccgatggac tacctgaatc
    14761 cagctcatcc gctgctgttg atctccttcg gtcgatccgg caacagcccg gaaagcgtcg
    14821 ctgccgtgga actggcaaat caatttgtac cggaatgcta tcacctgccg atcacctgca
    14881 acgaagcggg cgctctttac caaaacgcga tcaacagcga taacgcattt gccctgctga
    14941 tgcccgcaga aacgcacgat cgcggctttg cgatgaccag cagcattacc accatgatgg
    15001 ccagctgcct cgcggttttc gcacctgaga cgatcaacag ccaaaccttc cgcgacgtgg
    15061 cggatcgttg ccaggcgatc ctgacctcac tgggcgattt cagcgaaggt gtgtttggtt
    15121 acgcaccgtg gaaacggatc gtttatctcg gcagcggtgg cttacagggc gcagcacgcg
    15181 agtcggcgct gaaagtgctg gaactgacgg cgggtaaact ggcggccttt tatgattctc
    15241 caaccggatt ccgtcatgga ccaaaatcgc tggtcgataa cgaaacactg gtggtggtat
    15301 ttgtctccag ccacccttac acccgtcagt atgatcttga tctgctggct gaactccgcc
    15361 gtgacaacca ggcaatgcgc gtaatcgcca tcgccgcgga aagcaccgac atcgtcgctg
    15421 ccggtccaca tattatcctg ccgccgtcac gtcactttat cgacgttgag caggcatttt
    15481 gcttcctgat gtacgcccag acgtttgcac tgatgcagtc gctgcacatg ggcaatacgc
    15541 cggatacccc atcagccagc ggcaccgtta accgcgtggt gcaaggcgta atcattcatc
    15601 cgtggcaggc ataagaggat cgcattatga gcattatctc cactaaatat ctgttgcagg
    15661 acgcccaggc caatggctac gcggtgcctg cttttaacat tcataacgcc gagacgatcc
    15721 aggcgatcct cgaagtgtgc agtgaaatgc gatcgccggt gatcctcgcc ggaacgccgg
    15781 ggacctttaa acatatcgcg ctggaagaga tctacgccct gtgcagcgcc tattccacaa
    15841 cctacaacat gccactggcg ctgcatctcg accaccacga atcgctggat gatattcgcc
    15901 gtaaagtcca cgcaggtgtg cgcagtgcaa tgatcgacgg cagccacttc ccgtttgccg
    15961 agaacgtgaa gctggtgaaa tcggttgttg acttctgcca ctcacaggat tgcagcgtgg
    16021 aagcagaact gggccgcctg ggcggtgttg aagatgacat gagcgttgac gccgaaagtg
    16081 cattcctgac cgatccacaa gaagctaaac gctttgtcga actgactggc gtcgacagcc
    16141 tggcggtagc gattggtacg gcgcacggct tatacagcaa aacgccgaag attgatttcc
    16201 agcggctggc ggaaattcgt gaagtggtgg atgttcctct ggtgctacat ggtgccagcg
    16261 atgttccgga tgaatttgtc cgtcgcacta ttgaacttgg cgtcacaaaa gtgaacgttg
    16321 ccacagaatt aaaaatagcc ttcgctggcg cggttaaagc ctggtttgcg gaaaatccgc
    16381 agggtaatga tcctcgttat tatatgcgcg tcggaatgga tgcgatgaaa gaagttgtca
    16441 gaaataaaat taatgtctgt ggttcagcga atcgaatttc agcataatca ttagtttttt
    16501 atgatttatc ccaatgtact tcccgattaa tatcggggag tgccttaatg gaaaaggaga
    16561 tgactaaacc ttaataaata catcagtaca atatcgcaac aataatatat taaaaaaaat
    16621 tatattattc aactttatgg tgaggattac acaatgagca gtccaaatat tctcttaacc
    16681 cgtattgata accgtctggt tcacggtcag gttggcgtga cctggacatc caccatcggt
    16741 gcgaatctgc tggtagtcgt ggatgatgtt gtcgctaacg atgatattca acagaaatta
    16801 atgggtatta ccgcagaaac ctacggcttt ggcattcgtt tctttactat cgaaaaaacc
    16861 attaacgtca tcggcaaagc tgcaccgcat cagaagatct tcctgatttg ccgtacgcca
    16921 caaacggtac gtaaattggt agaaggtggc attgacctga aagatatcaa cgtcggcaat
    16981 atgcatttct cggaagggaa aaagcaaatc agcagtaaag tttatgtcga tgaccaggat
    17041 ctcgcggact tacgttttat taaacaacgt ggcgtgaatg ttttcattca ggacgtccct
    17101 ggcgatcaaa aagaacaaat ccctgactaa atctaaaatc gccttaatat tggtttgagg
    17161 taataaaaat gcatgaaata accctacttc agggattatc cctggcggcg ttagttttcg
    17221 ttctggggat tgatttttgg ctggaagcct tatttttatt ccgcccgata atcgtttgta
    17281 ccctaactgg cgctattctc ggtgatattc agactggctt aattaccggt ggtctgacag
    17341 agttggcttt cgccggatta acccctgcag gtggtgttca gccgcccaac ccgattatgg
    17401 cgggtctgat gaccaccgtc attgcatggt ctacgggcgt tgatgccaaa acagcaattg
    17461 gtcttggcct gccgtttagt ttgttaatgc agtacgtcat tctgttcttc tattccgctt
    17521 tctcattatt tatgaccaaa gccgataaat gcgcgaaaga ggcggatacg gcagcgtttt
    17581 cccggcttaa ctggacaacg atgctcatcg tcgcttcagc gtatgcggtg attgctttcc
    17641 tctgtactta cctggcacaa ggggcgatgc aggcgctggt gaaagcgatg cccgcctggt
    17701 tgacccacgg ctttgaagtg gctggcggta ttctgcctgc cgttggtttt ggcttgctgc
    17761 tgcgcgtaat gttcaaagcg caatatatcc cttacctgat cgccggtttc ctgtttgttt
    17821 gctacatcca ggtcagcaac ctgttgccgg ttgccgtact gggcgcaggc tttgcggtgt
    17881 atgagttttt caatgcgaaa tcccggcagc aagcgcaacc gcagcccgtt gccagtaaaa
    17941 atgaagaaga ggactacagc aatgggatct gaaatcagta aaaaagatat cacccgtctg
    18001 ggctttcgtt cgtcgctgct gcaagcgagc tttaactacg aaaggatgca ggcgggcggt
    18061 tttacctggg cgatgttgcc gatcctgaaa aagatttata aggacgacaa accgggctta
    18121 agcgcggcga tgaaagataa cctcgaattt attaataccc acccgaatct ggtcggattc
    18181 ctgatggggt tattaatttc gatggaagaa aaaggagaaa accgcgacac cattaaaggc
    18241 ctcaaagtgg cactgtttgg cccaatcgcc gggattggcg atgcgatttt ctggtttact
    18301 ttgttgccga ttatggcggg aatttgctca tcatttgcca gccagggaaa cctgctgggg
    18361 ccgattctat ttttcgccgt ttacctgctt atctttttcc tgcgcgtcgg ctggacccac
    18421 gtcggttatt cagtcggcgt gaaggcgatc gataaagtgc gagagaactc gcagatgatt
    18481 gctcgctcgg caaccatcct cgggatcacg gtaatcggcg ggctgatcgc ttcgtatgtg
    18541 catattaacg tggtgacatc gtttgccatc gacagtaccc acagcgtcgc gctgcaacag
    18601 gatttcttcg ataaagtctt cccgaacatt ttaccgatgg cctacaccct gctgatgtat
    18661 tacttcctgc gggtgaaaaa agcgcatccg gtgctgttaa tcggcgtgac ttttgtgctc
    18721 tctattgttt gttccgcatt cggcattttg taaatggaac gaggcactgc ttctggcggt
    18781 gcctctttac ttaaggaatt tcatcctgtg caaacccttc agcaagttga aaactatacg
    18841 gcgttaagtg aacgagccag cgaatattta ttggctgtga tccgtagcaa accggatgcc
    18901 gtgatttgcc tggcgaccgg agccacgcca ttactgacgt atcattatct ggtagaaaaa
    18961 atccaccagc agcaggttga tatcagccag ctcaccttcg tgaagctcga cgaatgggtg
    19021 gatctgccat taacgatgcc tggcacctgc gaaactttcc tacaacagca tatcgtgcag
    19081 ccgctggggc tgcgtgaaga ccagctcatc agctttcgct ccgaagagat aaatgagaca
    19141 gagtgcgaac gggtaacgaa cctgattgcg cgcaaaggcg gtctggattt atgcgttcta
    19201 ggattgggga aaaacggtca tcttgggctg aacgaaccgg gagaaagcct acaaccggcc
    19261 tgccatatca gtcaacttga tgccagaaca cagcaacatg agatgttaaa aaccacgggt
    19321 cgccccgtga ctcgtgggat caccttaggc ctgaaggata ttctcaatgc ccgcgaggtt
    19381 ttgttattgg tgactggcga aggaaagcag gatgcgacag aacgttttct cacggctaaa
    19441 gtctccaccg ctatcccggc ttcgttttta tggctgcaca gtaactttat ttgtttaatt
    19501 aatacctaac cagatgaaaa tttgctcaaa atttttaaat taacttattc aacagtcacg
    19561 cattatatta aataacattt gactgggttg aacataacgc caatagcaaa aggagttatg
    19621 tt
//
LOCUS       DAZGXZ010000096        19533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000096 DAZGXZ010000000
VERSION     DAZGXZ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.096"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            353..868
                     /gene="ehxC"
                     /locus_tag="ACHKJF_003540"
                     /gene_synonym="hlyC"
     CDS             353..868
                     /gene="ehxC"
                     /locus_tag="ACHKJF_003540"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFP0950841.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWLSGERKWFIDWIAPFGHNMDL
                     YKHMRKKYPYELFRAIRLDEYSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            870..3866
                     /gene="ehxA"
                     /locus_tag="ACHKJF_003541"
     CDS             870..3866
                     /gene="ehxA"
                     /locus_tag="ACHKJF_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP0950842.1"
                     /translation="MTVNKIKNIFNNATSTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADDLGIEIHRTERDDTAIANQFFGTAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PNLGPLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAIADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVADKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFVTPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSNLIQFVEKDNKHYQARIISELGDKDDIVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDRDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKADKIPHNNNERSGYIKASNIASEKNMVNITSVADDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3916..6036
                     /gene="ehxB"
                     /locus_tag="ACHKJF_003542"
                     /gene_synonym="hlyB"
     CDS             3916..6036
                     /gene="ehxB"
                     /locus_tag="ACHKJF_003542"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP0950843.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELGAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            6040..7479
                     /gene="ehxD"
                     /locus_tag="ACHKJF_003543"
     CDS             6040..7479
                     /gene="ehxD"
                     /locus_tag="ACHKJF_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP0950844.1"
                     /translation="MRFYMKGLWDLICRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVIWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGAFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESFRER"
     gene            7564..7764
                     /locus_tag="ACHKJF_003544"
     CDS             7564..7764
                     /locus_tag="ACHKJF_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0950845.1"
                     /translation="MVEVYFLNLKKVNGMNIIKLLNKVIIYQQQNKQSYPAQEHLLLQ
                     LCMRVTKKLTDNINSSLKGGYK"
     gene            7775..8143
                     /locus_tag="ACHKJF_003545"
     CDS             7775..8143
                     /locus_tag="ACHKJF_003545"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0950846.1"
                     /translation="MVLALLSSADNFFLSPTELSEKLDISRTNITRVFDSLEKFGFIR
                     RMESKEDRRSKNIYLTPDGDLLLQKITRIYGRYLKKYGATLQMIKSRYLSNQQKDVIS
                     KYFSVISHVIMHINPEFLFL"
     gene            8228..8458
                     /locus_tag="ACHKJF_003546"
     CDS             8228..8458
                     /locus_tag="ACHKJF_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFP0950847.1"
                     /translation="MWILNVFQVHKSVRIYIAHFSVFNCHLHPSIDRSLASWYYPNSP
                     KNGKPALILCHDWLNTGSLLQNAHLRIWFYPQ"
     gene            8579..9320
                     /locus_tag="ACHKJF_003547"
                     /pseudo
     CDS             8579..9320
                     /locus_tag="ACHKJF_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(9605..10582)
                     /locus_tag="ACHKJF_003548"
     CDS             complement(9605..10582)
                     /locus_tag="ACHKJF_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP0950848.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNI
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKISPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(11355..11630)
                     /locus_tag="ACHKJF_003549"
                     /pseudo
     CDS             complement(11355..11630)
                     /locus_tag="ACHKJF_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419664.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11659..12761
                     /locus_tag="ACHKJF_003550"
                     /pseudo
     CDS             11659..12761
                     /locus_tag="ACHKJF_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775007.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
     gene            complement(12783..13118)
                     /locus_tag="ACHKJF_003551"
     CDS             complement(12783..13118)
                     /locus_tag="ACHKJF_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFP0950849.1"
                     /translation="MNRKYYFNNMWWGWFMGGYTLYMSWDYEFKYRLLFWCISLCGMV
                     LYPVARWCIENTALKFTRPDFWNSGFFADTPGKMGLLAVYTGTVFILSLPLTLIYILS
                     VIIKRLSVR"
     gene            13247..13594
                     /locus_tag="ACHKJF_003552"
     CDS             13247..13594
                     /locus_tag="ACHKJF_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFP0950850.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLLPLPF
                     MPFWQVTVLMGGLWGISWGCAMWFIYWGLSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(13614..14204)
                     /locus_tag="ACHKJF_003553"
     CDS             complement(13614..14204)
                     /locus_tag="ACHKJF_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950851.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLIPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGIEVRVQINESAAP"
     gene            complement(14204..14461)
                     /locus_tag="ACHKJF_003554"
     CDS             complement(14204..14461)
                     /locus_tag="ACHKJF_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950852.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIDADFQRLRNLIWPENDR"
     gene            14790..15242
                     /locus_tag="ACHKJF_003555"
     CDS             14790..15242
                     /locus_tag="ACHKJF_003555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0950853.1"
                     /translation="MKFMECAVRDVIYGTNVRIVKPVNIYECELRDNVFVGPFVEIQK
                     GCVIGSGSRIQSHTFICENVTLGENCFIGHNVTFANDLFRSGAPDPSPDNWISIILGD
                     SVTVGSGAIILSPYICSGAVIGAGAVVVKPVENKGIYAGNPARLIRIL"
     gene            complement(15275..15637)
                     /locus_tag="ACHKJF_003556"
     CDS             complement(15275..15637)
                     /locus_tag="ACHKJF_003556"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950854.1"
                     /translation="MASLHVKRFYDSWLETIRIGLLSGLLPDPARDFSRYWNIISSMV
                     KPAYLATPPAFPEHGMMDSLFDFRIARIRILSGLGNDALGYLLKKGDITDAEYRAALE
                     IDPRQSISVHLPYSQTYL"
     gene            16069..16287
                     /gene="ccdA"
                     /locus_tag="ACHKJF_003557"
     CDS             16069..16287
                     /gene="ccdA"
                     /locus_tag="ACHKJF_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFP0950855.1"
                     /translation="MKQRITVTVDSDSYQLLRAYDVNISGLVSTTMQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            16289..16594
                     /gene="ccdB"
                     /locus_tag="ACHKJF_003558"
     CDS             16289..16594
                     /gene="ccdB"
                     /locus_tag="ACHKJF_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFP0950856.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGNESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            16595..17404
                     /locus_tag="ACHKJF_003559"
     CDS             16595..17404
                     /locus_tag="ACHKJF_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0950857.1"
                     /translation="MSGSVMHSQSAAMVPAAYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGVRINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDTWYVSQLQTMVATLKIPMERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGMTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKALQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPEVDAVAMLKNRQV"
     gene            complement(17653..17793)
                     /locus_tag="ACHKJF_003560"
                     /pseudo
     CDS             complement(17653..17793)
                     /locus_tag="ACHKJF_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(18233..18619)
                     /locus_tag="ACHKJF_003561"
     CDS             complement(18233..18619)
                     /locus_tag="ACHKJF_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP0950858.1"
                     /translation="MKFVSSQEMIEFHDRLISRDGGVAGMPEPGRADAIIHRVLNMYH
                     YEGVTDIMDLAAVYLVAIARGHIFNDANKRTALFVAQVFLKRNGIHIISSRISFDEMQ
                     IIALNAATGEYNWKMVSDHLKAIILN"
     gene            complement(18616..18840)
                     /locus_tag="ACHKJF_003562"
     CDS             complement(18616..18840)
                     /locus_tag="ACHKJF_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP0950859.1"
                     /translation="MFTCNYSHARDNLASLLELAVDENQPVEIQRRKGGAAVLVDKDA
                     FERYRKAALDAEFAEIMQSHGTAYTELSKR"
     gene            18925..19259
                     /locus_tag="ACHKJF_003563"
                     /pseudo
     CDS             18925..19259
                     /locus_tag="ACHKJF_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT         5626 a         3978 c         4603 g         5326 t
ORIGIN      
        1 cgcagggttg aaataaccgg acagagtgtt cgttaatatg cagtgtagcc ccggaatgtc
       61 cggggctttt ttattaatag gtgggtttta ctttgctgca tcctgtttga tatttctttg
      121 atgagaagtt tgtcgcaggg tgattcatct gaatgttata atataaccta tctaggtgtg
      181 cataagaata catagctaat gtattactgt ttctaatgtt gtttttttgt aggagggatt
      241 ctccttgcgg tagctttttg tttttagatg cttattgctt aaagaatata atttttatgc
      301 ttttatattg atttctttac atttactgag ttttcttatt tgtttaaaca ttatgaaatc
      361 taatgctttt gacgtcatgg ggaaggttgc atggctatgg gcctgttctc ctctgcataa
      421 aaaatggcca ttgtctgttt ttgctattaa tgtcatacct gctattcaga caaatcaatt
      481 tgctctctta ataaaggatg agcttcctgt agctttttgt agctgggcca gcttagatct
      541 ggagtgtgag gtaaaatata taaatgatgt aacatctttg tatgcaaaag actggttgtc
      601 gggagaaaga aagtggttta ttgactggat agctccattt ggacacaaca tggacttata
      661 taaacacatg aggaaaaagt atccttatga gttattcaga gcaataaggt tagatgagta
      721 cagtaaaaca gggaaaatag ctgaatttca tggagggggg atagataaaa aactggcatc
      781 aaaaatattc aggcaatacc atcatgaact tatgtctgaa gtaaaaaaca gacaagattt
      841 taattttaat attgagaaag aaaactaata tgacagtaaa taaaataaag aacattttca
      901 acaatgcgac atcgactaca aaatcagcat tcaatacagc atcatcaagc gtacgttccg
      961 ctggaaaaaa actcatatta ttaatacctg ataattatga agctcagggc gtgggtatta
     1021 atgagttggt caaagctgct gatgatcttg gaatagaaat acaccgtact gaacgagatg
     1081 atacagcgat tgcaaaccag ttttttggta cagcagaaaa agttgtagga ttaactgaac
     1141 gtggtgttgc aatattcgca ccacaacttg acaaacttct gcagaagtat cagaaagttg
     1201 ggagtaaaat aggaggaacc gctgaaaatg taggtaataa tctgggaaaa gccggaacag
     1261 ttctctcagc actacagaat tttacgggga ttgctttatc tggcatggct cttgatgaat
     1321 tgctgagaaa acaacgggaa ggagaggata taagtcagaa tgatattgcc aaaagtagta
     1381 ttgaacttat taatcagctt gtagatacag tatcaagtat aaacagtacc gttgattcat
     1441 tttctgagca gcttaaccag cttggctcat ttttatccag taaacctcgc ttaagttctg
     1501 ttggtggaaa attacaaaat ttaccgaacc tgggccccct gggggatggg ctggatgttg
     1561 tctccggaat tctttctgct gtatcagcaa gctttattct gggaaacagt gacgcacata
     1621 caggaacaaa agctgcagcg ggtatcgaac tgacaactca ggttcttgga aatgttggta
     1681 aagctgtttc gcaatatatt ctggctcaga gaatggcaca gggattatcg acaacagctg
     1741 caagtgcggg tctgatcaca tcggctgtta tgctggctat cagtcctctt tctttcctgg
     1801 ctattgcaga taaatttgag cgagctaagc agcttgaatc atattctgaa cgatttaaaa
     1861 aattgaatta tgaaggggat gctttactcg cagcctttca taaagaaacc ggagctatag
     1921 atgcagccct gacaacaata aatactgtcc tgagttctgt atctgcggga gttagtgccg
     1981 cctccagtgc atccctcata ggggccccga taagcatgct ggtgagtgca ttaaccggta
     2041 cgatatctgg cattctggaa gcatcaaaac aggctatgtt tgagcacgtt gcagataaat
     2101 tcgctgcccg gatcaatgaa tgggaaaagg agcatggcaa aaattatttt gagaatggct
     2161 atgacgcaag acatgctgcg tttttagaag attctctgtc tttgcttgct gatttttctc
     2221 gtcagcatgc agtagaaaga gctgtcgcaa tcacccagca acattgggat gagaagatcg
     2281 gtgaacttgc aggtataacc cgtaatgctg atcgcagtca gagtggtaag gcatatatta
     2341 attatctgga gaatggaggg cttttagagg ctcaaccgaa ggagtttaca caacaagttt
     2401 ttgatcctca aaaagggact atagaccttt caacaggtaa tgtatcaagt gttttgacat
     2461 ttgtaacacc aacatttacc ccaggagaag aagtcagaga aagaaaacag agtggtaaat
     2521 atgaatatat gacatctctt attgtaaatg gtaaggatac atggtctgta aaaggcataa
     2581 aaaatcataa aggtgtatat gattattcaa atttgattca gtttgttgaa aaggataaca
     2641 aacactatca ggcgagaata atttctgagc tcggagataa agacgatata gtttattctg
     2701 gggcaggctc atcagaagta tttgctggtg aaggtcatga taccgtatct tataataaga
     2761 cggatgttgg taaactaaca attgatgcaa caggagcatc aaaacctggt gaatatatag
     2821 tttcaaaaaa tatgtatggt gacgtgaagg tattgcagga agtcgttaag gaacaggagg
     2881 tgtcagtagg gaagcgaaca gagaaaatac aatatcgtga ttttgaattc agaaccggtg
     2941 gaattcctta tgatgtgata gataatcttc attctgttga agaactcatt ggcggaaaac
     3001 atgatgatga attcaaaggc ggtaagttta atgatatatt ccatggcgca gatgggaacg
     3061 attatatcga aggtaattat ggtaatgatc gactatacgg cgatgatagg gatgattata
     3121 tatccggagg acagggagac gaccagttat ttggtggtag tggaaacgat aaattgagtg
     3181 gaggggatgg taataattat ctgacaggag gaagcggtaa tgatgagctt caggcacacg
     3241 gagcttataa tattctgtca ggtggtactg gtgatgataa actttatggt ggtggtggta
     3301 ttgaccttct ggatggaggg gaaggtaatg actatctgaa tggtggtttt ggtaatgata
     3361 tttatgttta taggcaaaac tatggtcatc atacaattgc agatgaagga ggtaaaggag
     3421 atcgtctgca cttatctgat attagctttg atgatatcgc atttaagaga gttggaaatg
     3481 atcttatcat gaataaagcc attaatggtg tactttcatt taatgagtca aatgatgtca
     3541 atgggataac atttaaaaac tggtttgcga aagatgcctc aggagcagat aatcatcttg
     3601 ttgaggttat aacagataaa gatggtcgtg agataaaagc tgataagata cctcataata
     3661 ataatgaacg gtcaggttat ataaaagcca gtaatatagc atctgaaaaa aacatggtta
     3721 atatcaccag tgttgccgat gatattaaca agattatttc ttcagtttca gggttcgatt
     3781 caggtgatga acgattagca tctttatata atttatcctt acatcaaaac aacacacatt
     3841 caacaacttt aacgacaact gtctgagact aaaagcggag gtgatggcca ttccaggagg
     3901 aatggtcatc gttatataat gagtaaatgt agttctcata atagtctgta tgcactgata
     3961 ttgcttgcac aatatcataa tataactgtc aatgctgaaa ctataaggca tcagtataat
     4021 acccacacac aagattttgg ggtgactgaa tggttactgg cagcgaaatc cattggctta
     4081 aaagcaaaat atgtagaaaa acatttttcc agattgtcaa taatttcttt acctgcgttg
     4141 atatggcggg atgacggtaa gcattatata ttatctcgtg ttactaaaga ttcatcacgc
     4201 tatcttgttt atgatccaga acagcatcag tcactaactt ttagtcggga tgagttcgaa
     4261 aaactgtatc agggaaaagt cattctggtt acgtcaagag caacagtagt cggagagtta
     4321 gctaaatttg atttttcctg gtttatcccc tctgttgtga aatacaggag gattttactt
     4381 gaggtgttaa ctgtttctgc ttttattcag tttcttgcgt taataacacc tctttttttt
     4441 caggttgtaa tggataaggt tttagttcac cgggggtttt caacgttaaa tattatcaca
     4501 atagcattta ttatagtgat actttttgaa gtgatattaa ccggagccag aacttatatt
     4561 ttctctcata ctacaagtcg tattgacgtc gaactgggtg ctaagttatt cagacatttg
     4621 cttgcattgc ctgtttcata ttttgaaaat cgcagggtcg gagagaccgt tgccagagta
     4681 agggaactgg agcaaattcg taatttttta accggacaag cgttgacatc agttcttgat
     4741 ctgttttttt ctgtaatatt tttttgtgtc atgtggtatt acagtcctca attaacactg
     4801 gttatattat tgtcactacc ttgttatgtt atatggtcat tgtttatatc acccttatta
     4861 cgtcgacgtc ttgatgataa gtttctcagg aatgcagaaa atcaagcttt tcttgtcgaa
     4921 acggtaacag caataaatac aattaaatcc atggcagtat cgccacagat gattgctaca
     4981 tgggataaac aactggccgg gtatgttgct tccagtttca gggtaaatct ggttgcaatg
     5041 acagggcagc aggggataca gctgatacag aaaagtgtaa tggtaattag tctatggatg
     5101 ggagcacatc ttgtcatatc gggagagata agcatcgggc agttaatcgc gtttaatatg
     5161 cttgcaggtc aggtcattgc tcctgttatc agactggctc atctttggca ggattttcag
     5221 caagttggta tttctgttga gcgccttggg gatgtattaa atacaccggt agagaaaaag
     5281 tcaggcagaa atatactgcc ggaaattcag ggggatatcg aatttaaaaa tgtcagattt
     5341 cggtattctt ctgacggtaa tgttattttg aataatatta atttatacat atcaaagggg
     5401 gatgttatcg gtatagttgg ccgttctggt tcaggaaaaa gtacattaac aaaactgctt
     5461 cagcgctttt atataccaga gaccggacag attttaattg atgggcatga tttatcactt
     5521 gcagatccag aatggttacg acgccagatt ggtgttgtat tacaggaaaa tatactatta
     5581 aatcgtagta ttatcgataa tattacatta gcttctccgg ctgtatctat ggaacaggct
     5641 attgaggcag ccagacttgc aggtgcccat gattttatta cagaactaaa agaagggtac
     5701 aatactattg ttggagaaca gggcgttggt ctttcaggag ggcagcgcca acggatcgca
     5761 atagcccggg ctcttgttac aaatcctcga attcttattt ttgatgaagc aaccagtgct
     5821 cttgattatg agtcggaaaa tataataatg aaaaatatgt caagaatatg taagaacaga
     5881 accgtaatta ttattgcaca caggttgtca actgtaaaaa atgcaaacag aataattgtt
     5941 atggataatg gatttatttc tgaagatggc acacataaag agcttatctc caaaaaagac
     6001 agtttatatg catatttata tcagttgcag gcataacgta tgcgctttta catgaaaggt
     6061 ttatgggatt tgatttgccg ttataagacg gttttttctg atgtctggaa aattcgtcac
     6121 acacttgatg ctcctgtgag agaaaaagat gagtatgcat ttcttcctgc tcaccttgaa
     6181 ctcattgaaa caccagtatc cagacgttct cattttgtta tatggagtat tttattattt
     6241 gtaattatat ctcttctctt atctgttctt gggaaagttg aagtggtctc agtagcaaat
     6301 ggtaagttta ctcatagtgg aagaagcaaa gaaataaaac cgatagagaa tgcgattgta
     6361 gaaaaaataa tggtgaaaga tggcgctttt gtaaaaaaaa atgatccatt agttgaattg
     6421 acggtgcctg gtgttgaatc tgatatttta aaatcagaag catctttgtt gtatgaaaaa
     6481 acagaacaat atcgctacgc aatcctttct gaatcaatac aacggaatga gcttcctgaa
     6541 ataaggataa cagattttcc tggcggagaa gataatgcag gaggtgaaca ttttcagagg
     6601 gttagctcat taataaaaga gcaattcatg acatggcaga acagaaaaaa tcaaaagcag
     6661 ctcacattaa ataaaaaaat agttgaacgg gatgcagcgc ttgctcgtgt tagcctgtat
     6721 gagcatcagg tatcacagga aggaagaaaa cttaatgatt ttaagtattt gttgaataaa
     6781 aaagctgttt ctcaacattc agttatggag caggaaaata gctatattca ggcaaaaaat
     6841 gaacatgcag tctggcttgc acaggtttct caacttgaaa aagaaataga acttgtgcgg
     6901 gaagaactgg cactggagac gaatatcttc agaagtgaaa ttatcgagaa gcacagaaaa
     6961 tcaacagata acattgtgtt actggagcat gagcttgaaa aaaacaggca gagaaaagca
     7021 tcatctttta ttaaagctcc tgtgagtggt actgttcagg agttaaatat acatacagaa
     7081 ggtggtgttg tcacaacagc agaaacgctg atgattattg tccctgataa tgatattctc
     7141 gaagtaacag catctgtact taacaaggat atcggtttta tacaacctgg acaggaggtc
     7201 gttattaaag tagatgcata tccatataca cgtcatggtt accttacagg gaaagtaaaa
     7261 aatataactg cagattctgt ttctgttccg gatacaggac ttgtatttaa cgtgattata
     7321 tctgttgatc ggaatgatat acagggagaa agaaaaaaaa ttcctgttac agctggaatg
     7381 actgttatgg cagaaataaa aacaggagtg cgtagtgtta tcagctatct tcttagccca
     7441 ttaaaggaaa ctattaatga aagtttccgt gaacgttagg atataaataa catattggct
     7501 ttttatatag tatagatttc tgaaataatc ttgtaaatac tatcgttatt atttaaacaa
     7561 gccatggttg aggtttattt tttaaattta aaaaaagtta atggaatgaa tattattaaa
     7621 ttacttaata aagttattat ctatcaacaa cagaataagc aatcatatcc agctcaggag
     7681 catctccttt tacaactttg tatgcgtgtt accaaaaaac ttactgataa tattaattca
     7741 tccctgaagg gggggtataa atgatacaaa gcttatggtt ctggccctct tatccagtgc
     7801 tgataatttt ttcttatctc caactgaact cagtgaaaaa ttagatattt ccagaacaaa
     7861 tatcaccaga gtttttgatt ctctagaaaa atttggtttt attaggcgta tggaaagtaa
     7921 ggaagatcga cgtagtaaga atatttacct gactccagat ggtgatttgt tattacagaa
     7981 gattacaagg atatatggac gctatctaaa aaaatatgga gcaaccttac agatgataaa
     8041 gtcaagatat ttaagtaatc agcaaaaaga tgttatctca aaatatttct cagtaatatc
     8101 acatgtaatc atgcatataa atcctgagtt cttattttta taatttatta ttctgcaatt
     8161 gtgtgtgggg ggggagatac tggttattcc ccctcttttt tctggtaagg aaaatcagag
     8221 ttacgctatg tggattttga acgtatttca ggtgcataaa tctgtcagaa tctacatagc
     8281 gcatttttca gttttcaatt gtcacttaca tccatccatt gatagatcat tagcgtcctg
     8341 gtattacccc aattctccca aaaacggcaa accagccctc atcctgtgcc acgactggct
     8401 taatacggga tccctgctgc aaaacgcgca tttacgtatt tggttttacc cgcaataatg
     8461 cgtgataagc aagcaaaagg atccgcgtgc tgcgatttat tgtcgtggaa ggatccgggc
     8521 gttcaggtca gaatacctgt atccataaac agtgcgtgtg ctacgtgaaa aataactcat
     8581 gaacaatgtc attcccctgc agaattcacc agaacgcgtc tccctgttac ctattgcgcc
     8641 gggggtggat tttgcaacag cgctctccct gagaagaatg gcctcttcca cgggggccac
     8701 accggcctac ctgctggccc cggaagtgag tgcccttctt ttctatatgc cggatcagcg
     8761 tcaccatatg ctgttcgcca ccctctggaa taccggaatg cgtattggcg aagcccggat
     8821 gctgacgccg gaatcatttg atctggatgg tgcaagaccg tttgtgcgga tcctgtccga
     8881 aaaagtgcgt gcgcgacgcg gacgcccgcc aaaagatgaa gtccgcctgg ttccgctgac
     8941 agatataagc tatgtcaggc agatggaaag ctggatgatc accacccggc cccgtcgtcg
     9001 tgaaccatta tgggccgtga ccgacgaaac catgcgcaac tggctgaagc aggctgtcag
     9061 acgggccgaa gctgacgggg tacacttttc gattccggtc acaccacaca ccttccggca
     9121 cagctatatc atgcacatgc tctatcaccg ccagccccgg aaagtcatcc aggcactggc
     9181 tggtcacagg gatcccacgt tcgatggagg tttataccag agtttttgcg ctggatatgg
     9241 ctgccacgct ggcagtgcct ttcacaggtg acgggcggga tgctgcggag atcctgcgta
     9301 cactgcctcc cctgaggtga cggcatacgc gcttccttat ataagctgtg gtcagcagaa
     9361 cagacataac ataagctgga gcatgtatat aagctgtagt gagtaagtca tgttttatga
     9421 agggagcaat gcctcagcat caggttacgg gatcacttac gtaagggaca ggcagatagc
     9481 agctcagcta cagacagcac tgcaggaaac agaatataaa ccgcagtgag cagacaactc
     9541 actgcaccgg ttaatataag ctgtagtcag taaaggagcg ctctttactg actacagttt
     9601 atattcagcg ggatttgaag agtttttcca ggtcatccag cgtgatgccc agtttctcaa
     9661 gcagggcaag cttctccgcc atctccggac tgattttttc cgcaggtgaa ggtggcaacg
     9721 gattttcctt actctcatca ttcggtgctt ttaaccgggg acgccggtag tgaatgcaga
     9781 agagttttgt ccgcccccgc tggatctccg tgtaatcaag atatccaatc tcgcgcaact
     9841 gctccattgc ccgtctgacc gtctggttct gggaaaatac aggagacttc agattgaggc
     9901 gtgcacgcag ccgcgccagc gatatcggtg ccggatcccg gggcaggctc tctataaagg
     9961 tgtagagtgc ctgggcggac tcccgtcgct tcagggcatt aatcgcctta agctggagaa
    10021 ggacttttct gtcaaactgg tacagttcaa acaggcgggg atcggcctgt aactgaacaa
    10081 tatcccgctc agtatcgtag taggctgact gtaccagatg ggtgatgtat tcccgggtgt
    10141 gcttctcatc ggtacgggaa aacgagatca cggtaccggc aatgcgcttc agggaagggc
    10201 tgatgcgctc acgcagcctg cgggatgact ggcttgaagg tataccacac agttttgcaa
    10261 actcaacaaa aggcagttca actttgtcac caatcacgtt atggcgggca aaggaatgaa
    10321 tgatccccac ccaggtcttg aaatcgttat ccatatccag gcgggggccg gtgatctcaa
    10381 ccttatcgaa tccctcagca cgggccaagg aaagacgtgt cagctcttcc gtggcatcag
    10441 tacgtgacag tatatttttt ttactgttct tcagtgattt aagggtcggt acaaaaacgc
    10501 ccagacgcat cagcgccaca ggttgtacgg tgttgttatt gtttggtgtc agtgtcacca
    10561 gctcaccgga cgacttatcc acctcgccga acagtttttt gatgtctgaa ttttcgtttt
    10621 ccagaaagcc tcctgcctgt ggacagtaaa agaataaaca gaaaatcgga tgtcagaacc
    10681 ccgctaactg actgaagaaa aatctgcagc ctgtatttaa cagctgagtc tggcgtactg
    10741 accatagttt atgtcattaa cttactgcag tatatgtatt acttaataca gcttatcatt
    10801 aacttgttac agtttatcag taacatgcca cagcttatgt gatcgtgctc ccggaccatt
    10861 aatgacaagg tttacagcga tatggggatc tttaatggat cctttgttga tcattcatgg
    10921 atctgtttac tggatcgaac ctctggatat gtggataaat catcccaggg gaaacagcat
    10981 acacaaccgc ttataagggc ttcagggcgt acctgctcac tgtaactaca gatacattgc
    11041 gcttcctcgc cggttcgcgg gcttcgcccg gtcaccggtc tgcggcgagc actgtttttt
    11101 ctgaagtctg tctttacagg taatttttga cggaatctgc agtacttcct gagcgtacgg
    11161 tcatatccga agtacacttc atatgtgtgt ttcaatgcca gggattgtgt atacaaacta
    11221 caggcaaagt caggttatca ccggtggtag gctgctccga tggaaaaatt gtgaactgat
    11281 tgacaccatg cctgataact ctgtcgtgtt gtcagcgcca gtgatataag acggtcaaac
    11341 atcgtggcgt tgacacatac agagagccgg atgctgtttg ctgatgctga ttctcttcgt
    11401 atatctcccc gcgaggccag gtcgttaatt gaacaggctg aaaaacggca gaaggatgcg
    11461 cagaacgcag acaagaaggc cgctgatatg cttgctgaat acgagcgcag aaaaggtatt
    11521 ctgaatacgc ggttgtcaga gctggaaaaa aagggcgggg cagcccttgc cgttcttgat
    11581 gcacaacagg cccgtctgct cgagcagcag acacggaatg acagggccat ttcagaggcc
    11641 cggaataaac tcagttcggt gacggaatcg cttaacacgg cccgtaatgc attaaccaga
    11701 gctgaacaac agctgacgca acagaaaaac acgcctgacg gcaaaacgat agtttcctct
    11761 gaaaaattcc cggggcgttc atcaacaaat cattctattg ttgtgagcgg tgatccgaga
    11821 tttgccgata cgataaaaat cacaaccagc gcagttatcg ataaccgtgc aaacctgaat
    11881 tatcttctga ctcattccgg tctggactat aaacgcaata ttctgaatga ccggaatccg
    11941 gtggtgacag aggatgtgga aggtgacaag aaaatttata atgctgaagt tactgaatgg
    12001 gataagttac ggcaaagatt gcttgatgcc agaaataaaa tcacctctgc tgaatctgcg
    12061 gtaaattcgg tgagaaataa cctcagtgcc agaacaaatg agcaaaagca tgcaaatgac
    12121 gctcttaatg ccctgttgaa ggaaaaagag aatacccgta accagcttgc cggcatcaat
    12181 cagaagatag cggaagagaa aagaaaacag gatgaactga aggcaacgaa agacgcaatt
    12241 aatttcacaa cagagttcct gaaatcagtt tcagaaaaat atggtgcaaa agctgagcag
    12301 ttagccagag agatggccgg gcaggctaaa gggaagaaaa tacgtaatgt tgaagaggta
    12361 ttaaaaacgt atgaaaagta ccggactgac attaacaaaa aaattaatgc aaaagatcgt
    12421 gcagcgattg ccgcagccct tgagtctgtg aagctgtctg atatatcgtc taatctgaac
    12481 aggttcagtc gcggactggg atatgcagga aaaattacaa attttgctga ctggatcact
    12541 gagtttggta aggctgcccg gacagataac tggcgtcctt ttttgttaaa acagaaacca
    12601 tcatagcagg taatgccgca acggctcttg ttgctctggt cttcagtatt cttaccggaa
    12661 gcgctttagg cattatcggg tatggtttac tgatggctgt caccggtgca ctgattgatg
    12721 aaacgcttgt ggaaaaagcg aataagttct ggggcattta aataatatat aagacaggct
    12781 gtctatctta cagacagcct ttttatgata acagaaagaa tatatatcag agttaacgga
    12841 agagataata tgaaaacagt acccgtataa accgcaagta atcccatttt tccgggagta
    12901 tcagcaaaaa aaccgctgtt ccagaaatca ggccgggtaa atttcagggc agtattttca
    12961 atacaccatc tggcaaccgg atacaaaacc attccgcaga gagaaataca ccagaacagc
    13021 aatctgtatt taaactcata atcccatgac atatacagag tatacccacc cataaaccat
    13081 ccccaccaca tattattaaa ataatatttt ctgttcatct gcctgttttc ctttcttttg
    13141 cacttttgtc aatgtactga tgcatgacaa cataaccaca gcatgataat aataatcaat
    13201 aacaataagt tgtgtcacgt ttacatcatg gaacggaggt gctcctgtga cctttgagca
    13261 gaaaaaagcc cgggccattg ccctgatgga cagcaaaaag atgtggcgga gcaattatgc
    13321 ccctcctctt ctgcgtattc tctggcggct gggtatccgg cttctgccat taccctttat
    13381 gccgttctgg caggtcacgg tgctgatggg gggcttatgg ggcatttcct ggggatgtgc
    13441 aatgtggttt atctactggg ggctgtcagg aatggtggca ggtgaggcca ttattataag
    13501 tatcacgggg ggttttttgt tcggactgtg catggcctct ttccactggt ggcgcaggaa
    13561 agttaaccgg ctgccaccat gggatgatgt gtaaaccgtg cacaggcgtg ttcttacgga
    13621 gcggcacttt catttatctg tacgcgaact tctattcctg cgtcatgaca ggcctgcagc
    13681 cactgcgcca cttccagcgg atcgccctcc cggcgtacca ccctgccttc tttattccat
    13741 aactgcagac aggtgctgcc gtcgagacgc accacaaaat ccccacggca ggcctgatac
    13801 gttatgccgt tcagctccac cgccagatca gtggcagaag tgccgtctgc gggcagacct
    13861 gctgccggga tcagcggcag tacgacgact tctgccgcac gctgttctgc ccggaggcgg
    13921 tcctgttctg ccagcagcag aggctgtgcc acggcccggc cggcgtccgt cagctccact
    13981 gccagctgca ggttcggagc gcgcagggta cgcagccagc cggcatcctc cagacgacgg
    14041 caggaggcgc gcaggtttgg cccgtagacc ggggcttccc cgcttcgctc cagcacccgt
    14101 tctatgtccc gcgtcagcac ggggcccggc cgtttttcat caagggcagc cagcacaatc
    14161 agcactctcc gctgcagtgg cgacggtcgg cggctctggg tcattacctg tcattctccg
    14221 gccagatgag gtttctgagg cgctgaaagt cggcgtcaat gagctcctgt ctctttatgg
    14281 cattcattga ggcatagcca tttatggcca gttcatgcca gaagatcagc acggctccgg
    14341 ctttggctga ctcgcagaga taaatcgcgc cctggctgtc cagattgcat tcccggatgc
    14401 gtttttccgc aaaccgggtc agttgttcat agctgagtga aaacaccctt tcttcatcca
    14461 tcctgcgcgc tccatctgat aatgttctga caggtaaaaa tcaaattcta tatgattttt
    14521 gtgttatcga tttctgataa tatcagatat tatttcatta ctgatgatta aaatcatatc
    14581 tgctatgaat ttatgtgtgt tgcttaatta tcatatcacc tgcccgtttc ccgccgcgca
    14641 gatccggttt ccggctgaag tggatccaga cctgatttgt tgctgagtat cggtaaaagt
    14701 gcggcttata agctattctg aaacatggca actgaatctg ggctgtataa ttccggcaag
    14761 tgtcatctgt atttaaagga cgtgtgagta tgaaatttat ggaatgtgct gtccgggatg
    14821 tgatttatgg gaccaatgtc cgtattgtca aaccggtcaa catttatgaa tgtgaactca
    14881 gggacaatgt gtttgtcggc cccttcgttg agatacagaa aggctgtgtc attggtagtg
    14941 gaagtcggat acagtcgcac acgtttattt gtgagaacgt aacactgggg gagaactgct
    15001 tcattggtca taacgtgacg ttcgccaacg atctgtttcg ttcaggagca ccagatccgt
    15061 caccggataa ctggatcagc attatcctgg gggattcggt tactgttggc agtggagcca
    15121 ttattctgtc tccgtatata tgcagcggag ctgttatcgg tgcgggggca gttgtagtaa
    15181 aaccggttga gaacaaaggc atttatgcag gaaacccggc aagactgatc agaatattat
    15241 gaaaaaagaa aagggccgtt actggccctt tttatcagag gtaggtctgt gaatacggca
    15301 ggtgtacaga aatcgactgc cggggatcga tttccagggc cgcccggtat tctgcgtcag
    15361 tgatatcacc ttttttcagt aaataaccga gagcatcgtt tcccagccct gacagtatgc
    15421 gtattctggc gatccggaaa tcaaacaggc tgtccatcat accatgctcc ggaaatgcgg
    15481 gaggagtggc cagataggca ggtttgacca tactgctgat gatgttccag tatcgggaaa
    15541 agtcgcgggc cgggtccggg agaagacctg acagcagccc aatccggatg gtttccagcc
    15601 agctgtcata aaatcgctta acatgaagtg aagccatgtg ctcagcacac catttgcgca
    15661 tagcttcttg tcacggatct tcctccccct ccgcacagct ccacaccctg taagcacaac
    15721 atgtcgtgct gcattcagct gctcatgaca ctatatgttg tgtcatctcc ctgtcctgtg
    15781 atgtgccgcg caggggctga aaacagcaat atgatctatt cctcagcgtg gtacacttcc
    15841 ggaaagtcgt ttattcaaat aaagtcggat ccatacgaaa cgggaatgcg gtaattaagc
    15901 tttgttttta taagtcaggt tttaattttt actggttaac ataacgcacg gtaaaatata
    15961 taaggcttat taaaagccag ataacagtat gtgtattgac gcgctgattt gctgcataag
    16021 aatatatact gatatgcata ccagaagtat gtcagaaaga ggtgtgctat gaagcagcgt
    16081 attacagtga cagttgacag cgacagctat cagctgctca gggcgtatga cgtgaatatc
    16141 tccggtctgg tcagtacgac catgcagaac gaagcccggc gactgcgcgc tgaacgctgg
    16201 caggctgaaa atcaggaagg gatggcagag gtcgccaggt ttattgaaat gaacggctct
    16261 tttgccgatg agaacaggga ctggtgaaat gcagtttaag gtttacacct ataaaagaga
    16321 gagccgttat cgtctgtttg tggatgtcca gagtgacatt attgacacgc ccgggcgacg
    16381 gatggtgatc cccctggcca gtgcacgtct gttgtcagat aaggtctccc gtgagcttta
    16441 cccggtggtg catgtcggga atgaaagctg gcgcatgatg accaccgata tggccagtgt
    16501 gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgggaaa atgacatcaa
    16561 aaacgccatt aacctgatgt tctggggaat ataaatgtca ggctccgtta tgcacagcca
    16621 gtctgcagcc atggtaccgg cagcgtattc tgccggacag tctgcacaac ttcctgttgc
    16681 cattgattat ccggcagctc tggcactccg ccagatgtcg atggttcatg atgaactgcc
    16741 gaaatatctg ctggcccccg aagtgagtgc cctgctccat tacgtcccgg atctgcgtcg
    16801 caagatgctg ctggccacac tgtggaacac cggcgtgcgc attaatgaag cactggcact
    16861 gacgcgcggg gatttttcgc ttgcgcctcc gtatccgttt gtgcaactgg ccactctgaa
    16921 gcagcggaca gaaaaagccg ccaggacggc aggaagaatg ccagccggtc agcagactca
    16981 ccggctggtt ccgctctccg acacctggta cgtcagccag ctgcagacga tggtggccac
    17041 actgaaaatc cccatggaac ggcgtaataa acgaacaggc aggacagaga aagcgcggat
    17101 ctgggaagtg acggacagaa cggtcaggac ctggattggg gaggcggttg ccgccgctgc
    17161 cgctgatggt atgacgttct ctgtcccggt cacgccacat acgttccgcc attcctatgc
    17221 gatgcacatg ctgtatgccg gtataccact gaaggccctg cagagcctga tgggacataa
    17281 gtccatcagt tcaactgaag tctacacgaa ggtgtttgca ctggatgtgg ctgcacggca
    17341 ccgggtgcag tttgcgatgc ctgaagtgga tgcggtagcc atgctgaaaa acaggcaggt
    17401 ataattgggg gcagtttgga tatagagaat tattgtacgg caagcctgtt ttttgaacag
    17461 acctgcctgt cgcaataggg tgtactcccg tgctatacgg cgttttcagg gaatttggcc
    17521 ttaatcggat aacctaaatg cgatatagca atacaggcga cagccactga cgttaaatca
    17581 atacgtcagg atcttaaatt tgttaaataa tgcccttaca gtacaaaaat ttccgtttcc
    17641 caaacagccc cctgcattac cgatacaggc aaccagcgca caggcagttg tgggaccaat
    17701 tccttttagc tccatcagcc gctgactgcg gtgatctgtt ctggccatac gggacaaaat
    17761 tttgtcatat tcagtgatgt tagcttcaat gcggggtcag tttggagatc gtaaaatttt
    17821 gttcgttaag cacattattt aaccaataga tatgcgtaac ggattgattt tgcgtcaacg
    17881 gctatcgctt ttaagttatc gccattgagg tattgcattt aggttatccg atagaggtca
    17941 aatcctctga aaacgccgta tagcacggga gtacacccta ccgcaatagc gcaaccgtac
    18001 aatattgtac gtttcccaaa cgcagcctag gaagcacgtc gtcagtattg gagaaaactt
    18061 acaaacgctc gccgcagccc cagcgaccga acgttagtga gcgagtgggc gaggaagcgg
    18121 aaaagtgtca ggtctgaatt gagcacatac gccagaaatt atatagcaaa aagatatatg
    18181 atttttacat tgctcagtag attgtaaaat gctgctgatt ggatatctat atctaattta
    18241 aaataatggc tttaagatgg tcagaaacca tcttccagtt atattctcct gttgcagcat
    18301 tcaaagcgat aatttgcatt tcatcaaacg aaatacgcga actaataata tgtataccat
    18361 tccgttttaa aaaaacctgt gcaacaaaca aggctgttcg cttatttgca tcgttgaaaa
    18421 tgtgaccacg agcaatagca actaaataca cggcagccag atccataata tctgtcacac
    18481 cttcataatg atacatgttc aaaacacgat gaatgatcgc gtcagcgcga ccgggttctg
    18541 gcatacccgc cacgccacca tcacgcgaaa tcaagcggtc atgaaactca atcatttcct
    18601 gtgaactaac gaatttcatc gcttagaaag ctccgtatag gccgtcccat gactttgcat
    18661 gatctcagca aactcagcat caagagccgc tttacggtat cgttcaaaag cgtctttatc
    18721 aaccagtaca gccgcgccac ctttacggcg ctgaatctca acaggctgat tttcatcaac
    18781 cgccagctcg agcaacgagg ccaggttatc gcgagcatgg ctatagttac atgtaaacat
    18841 agtgcttcct ctttgagttg tacaattaaa ttgtacaaca gttatcttca atatgaaatc
    18901 accgttaccg ctggcgcatc ctgccaactt aaccggggag aaagtcgcca tgccgttgcc
    18961 agagctatct gtcacggtca gaaaggtgag ataagaaagc gatataccga tggtcaagaa
    19021 gatcaactgg gcgcactggg gctggtgact aacgccgtcg tattatggaa cactatttat
    19081 atgcaggctg ccctggacca tttcagggca cagggggaaa tcgtgaatga tgaagatatc
    19141 gcacgcctct caccactttg ccacggacac atcaatatgc tcggccatat tccttcacgc
    19201 tggcagaact ggtgactaaa gggcaaaaag aggcatcgga ggaagaaaac attgcttaac
    19261 gtgagttttc gttccactga gcgccagccc cctaattctc tatatccaaa ctgaccccaa
    19321 taggtgacgg gttatgtgac gaaatctgat gtagaaatcg ttgtttcagt gacagtcact
    19381 caatcggtca tttatgtgac accccacgcc gttactggtc gcggaaaaat ccagcggaaa
    19441 aatccaggtt tccggcttgg aaccccatct caagagatcc tttgatcttt tctactgaac
    19501 cgcgatcccc gtcagttcag aagacgaaga tga
//
LOCUS       DAZGXZ010000097        19119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000097 DAZGXZ010000000
VERSION     DAZGXZ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.097"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..434)
                     /locus_tag="ACHKJF_003564"
     CDS             complement(<1..434)
                     /locus_tag="ACHKJF_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0950860.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTG"
     gene            504..866
                     /gene="tnpA"
                     /locus_tag="ACHKJF_003565"
                     /pseudo
     CDS             504..866
                     /gene="tnpA"
                     /locus_tag="ACHKJF_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(930..1223)
                     /locus_tag="ACHKJF_003566"
                     /pseudo
     CDS             complement(930..1223)
                     /locus_tag="ACHKJF_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            1495..2289
                     /gene="yjfZ"
                     /locus_tag="ACHKJF_003567"
     CDS             1495..2289
                     /gene="yjfZ"
                     /locus_tag="ACHKJF_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0950861.1"
                     /translation="MTLPTTIYSFPAYSPHFSSTDKPVKLKFHQYARATLLPNRGRDH
                     NCDGRRTVEIHKLDLSDWQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKY
                     NKAHAGARLTAELKYLLQDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPA
                     QMPNCWSSELPTIDLYIDQLRTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNS
                     AAIYSGLGFERVPQDRDIRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(2360..2809)
                     /gene="rplI"
                     /locus_tag="ACHKJF_003568"
     CDS             complement(2360..2809)
                     /gene="rplI"
                     /locus_tag="ACHKJF_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP0950862.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(2851..3078)
                     /gene="rpsR"
                     /locus_tag="ACHKJF_003569"
     CDS             complement(2851..3078)
                     /gene="rpsR"
                     /locus_tag="ACHKJF_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP0950863.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(3083..3397)
                     /gene="priB"
                     /locus_tag="ACHKJF_003570"
     CDS             complement(3083..3397)
                     /gene="priB"
                     /locus_tag="ACHKJF_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP0950864.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(3404..3799)
                     /gene="rpsF"
                     /locus_tag="ACHKJF_003571"
     CDS             complement(3404..3799)
                     /gene="rpsF"
                     /locus_tag="ACHKJF_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP0950865.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            4126..4401
                     /gene="yjfY"
                     /locus_tag="ACHKJF_003572"
     CDS             4126..4401
                     /gene="yjfY"
                     /locus_tag="ACHKJF_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP0950866.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(4476..5027)
                     /locus_tag="ACHKJF_003573"
     CDS             complement(4476..5027)
                     /locus_tag="ACHKJF_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP0950867.1"
                     /translation="MAIKGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCVV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(5122..5808)
                     /gene="ulaF"
                     /locus_tag="ACHKJF_003574"
     CDS             complement(5122..5808)
                     /gene="ulaF"
                     /locus_tag="ACHKJF_003574"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP0950868.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARGINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(5808..6662)
                     /gene="ulaE"
                     /locus_tag="ACHKJF_003575"
     CDS             complement(5808..6662)
                     /gene="ulaE"
                     /locus_tag="ACHKJF_003575"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP0950869.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(6672..7322)
                     /gene="ulaD"
                     /locus_tag="ACHKJF_003576"
     CDS             complement(6672..7322)
                     /gene="ulaD"
                     /locus_tag="ACHKJF_003576"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0950870.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(7336..7800)
                     /gene="ulaC"
                     /locus_tag="ACHKJF_003577"
     CDS             complement(7336..7800)
                     /gene="ulaC"
                     /locus_tag="ACHKJF_003577"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP0950871.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(7810..8115)
                     /gene="ulaB"
                     /locus_tag="ACHKJF_003578"
     CDS             complement(7810..8115)
                     /gene="ulaB"
                     /locus_tag="ACHKJF_003578"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP0950872.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(8131..9528)
                     /gene="ulaA"
                     /locus_tag="ACHKJF_003579"
     CDS             complement(8131..9528)
                     /gene="ulaA"
                     /locus_tag="ACHKJF_003579"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0950873.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9883..10947
                     /gene="ulaG"
                     /locus_tag="ACHKJF_003580"
     CDS             9883..10947
                     /gene="ulaG"
                     /locus_tag="ACHKJF_003580"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP0950874.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            11055..11810
                     /gene="ulaR"
                     /locus_tag="ACHKJF_003581"
     CDS             11055..11810
                     /gene="ulaR"
                     /locus_tag="ACHKJF_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP0950875.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(11807..12556)
                     /gene="yjfP"
                     /locus_tag="ACHKJF_003582"
     CDS             complement(11807..12556)
                     /gene="yjfP"
                     /locus_tag="ACHKJF_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0950876.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSCLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            12738..13067
                     /gene="bsmA"
                     /locus_tag="ACHKJF_003583"
     CDS             12738..13067
                     /gene="bsmA"
                     /locus_tag="ACHKJF_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP0950877.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            13216..13491
                     /gene="yjfN"
                     /locus_tag="ACHKJF_003584"
     CDS             13216..13491
                     /gene="yjfN"
                     /locus_tag="ACHKJF_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP0950878.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(13608..15233)
                     /gene="aidB"
                     /locus_tag="ACHKJF_003585"
     CDS             complement(13608..15233)
                     /gene="aidB"
                     /locus_tag="ACHKJF_003585"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP0950879.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(15317..16480)
                     /gene="yjfC"
                     /locus_tag="ACHKJF_003586"
     CDS             complement(15317..16480)
                     /gene="yjfC"
                     /locus_tag="ACHKJF_003586"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0950880.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPCDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(16483..17121)
                     /gene="yjfM"
                     /locus_tag="ACHKJF_003587"
     CDS             complement(16483..17121)
                     /gene="yjfM"
                     /locus_tag="ACHKJF_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP0950881.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEQFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(17131..17529)
                     /gene="yjfL"
                     /locus_tag="ACHKJF_003588"
     CDS             complement(17131..17529)
                     /gene="yjfL"
                     /locus_tag="ACHKJF_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP0950882.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(17547..18206)
                     /gene="yjfK"
                     /locus_tag="ACHKJF_003589"
     CDS             complement(17547..18206)
                     /gene="yjfK"
                     /locus_tag="ACHKJF_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP0950883.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(18257..18955)
                     /gene="yjfJ"
                     /locus_tag="ACHKJF_003590"
     CDS             complement(18257..18955)
                     /gene="yjfJ"
                     /locus_tag="ACHKJF_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP0950884.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(18974..>19119)
                     /locus_tag="ACHKJF_003591"
     CDS             complement(18974..>19119)
                     /locus_tag="ACHKJF_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP0950885.1"
                     /translation="SSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEE
                     YSH"
BASE COUNT         4921 a         5058 c         4522 g         4618 t
ORIGIN      
        1 cccgtgacct gacggctatt ccggtagcgc atccagccaa gtttcggcag aaaaatacgg
       61 ctgttttcct gatcgagctt aacgccctgc gggtagcgga atgcatcatt ctgtccccgc
      121 tttttgaatc ggggaaaagc agcccgcttc tggaagaagt ttttgtaagc ccgctcaagg
      181 cctttcagtg actgttgcaa tggctgtgag ggcgaatctt taagccattg cgtttcagtg
      241 gcgtttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta tttattaccg
      301 gcctcatgat tctcattctg aagcgccaga gcacgattga aaacgaaacg acatgcgcct
      361 gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaactggaa tttaaatgcc
      421 tgtaatcgtt tcacaggcca aatgatactt tggtctatga ataaagaaac cgatatccga
      481 cggggaagca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc agataccgac
      541 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      601 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      661 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      721 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      781 tgtggagtcc tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc
      841 aatacattga acaacagcaa acacctggtc aggtggaaaa ccgcgcctta tatccccgcc
      901 ctgaaggacg gggttttacg gcgcaccaga taaaccaggc caagcgtatg ctggttgatc
      961 aaataatatc ccccaagcgc agcaatatta atattcacct gcaatgggtc gatacctggc
     1021 ttaaaaatat tgtatttctt cccttcatcc aataaggatt ccattaactg caagtgcgca
     1081 tggttaattt ctaagagacg ctgtgatttt gcataatgca cacctttact ttgattttca
     1141 ctatgcacaa tttttaaaaa ccaagggtta gcaatataat aatcccatgt aaaatcaata
     1201 attttctcaa tggctttttg tggctggtta accagatgat taacaaacgc accgatacca
     1261 acagcgtaga ataattacat accgcacaac aagaatgtat ggtggaaggc gtggaagtca
     1321 ttttctcccc gggggcatcg ttatatccat aatgagcgat ttttgatatt aatatctttc
     1381 accatccgct attttcacta cttcagaaaa caaacttatt tttattaaaa tcgattcttt
     1441 ttctgtgaac atcagctgcg atatttataa aaacattaaa caggtaacat tacaatgaca
     1501 ttaccaacga ctatttattc atttcctgca tattcacccc atttttcgtc caccgacaaa
     1561 ccagtaaaat taaaatttca tcaatatgca cgtgctacat tactgccaaa tcgtggaaga
     1621 gatcataatt gcgatggcag acgtactgtt gagatccaca aactagatct ctctgactgg
     1681 caagcattca ataagttagc aacgaagtgt aatgcttacg atggagtaac aatgaatggt
     1741 gataattctt ttggatggaa ccatgaagct acattagata atattcacgc ccagaaatac
     1801 aataaagctc acgcgggtgc gaggttaacc gcagaattga aatatttatt gcaagatgtc
     1861 gaatccttcg aacccaacag taaatacaca atacacgaag tggttttggg acctggttat
     1921 ggtactccag actataccgg ccaaacgata ggatacgttg taacccttcc agcccaaatg
     1981 cctaattgct ggtcatctga actaccaaca atcgatcttt atatcgacca acttcgcact
     2041 gttacaggcg tttctaatgc cctcggattc atcattgctg cattgctaaa cgcgtattct
     2101 gatttacccc atgatttgaa aattggtcta cgttccttat caaattcagc agcaatctac
     2161 tcgggtttag gtttcgagcg agtgccgcag gaccgagaca taaggagtga tcgtatgcat
     2221 ctaacgccag caaaccatcc tgacttatgg acacaagaga atggtgaatg gatctacttg
     2281 cgaaactaat acctgacgaa aaatagaaag caaaacgccg accaatggtc ggcgttttta
     2341 cgtctcgttg aataacgaat tattcagcta ctacgtttac gatcactttc gcgaatactt
     2401 cgctgtgaac ctggaagctc acttcgtgtt cgccagtggt acgcagaacg ccgttcggca
     2461 gacgaacttc gctcttagcc acttcaacgc cagctgcagt tacagcgtca gcgatgtcgc
     2521 gagtaccgat ggaaccgaac agtttacctt cgtcgccagc tttagacgcg atggtaacag
     2581 tttccagtgc attgattttc tcagcgcgag cattagcagc tgccagaact tcagccagtt
     2641 tagcttccag ttcagcgcga cgtgcttcga agaattcaat gtttttcttg gtagctggaa
     2701 cagctttacc ctgcggtacc aggaagttac gagcatagcc cgctttaacg tttacctgat
     2761 cacccaggct acccaggttt gctactttat caagcagaat aacttgcatt accttatcct
     2821 ctcaaagtcg tattaatgga ccgtgaccga ttactgatgg cgatcagtgt acggcagcag
     2881 ggacaggtag cgagcgcgtt tgatagcgcg agccagctga cgctggtatt ttgcacgggt
     2941 accggtgata cggcttggga caatcttacc gctttcggtg atgtagtttt tcagcgtagc
     3001 gatatcttta tagtcgatct cttgaacgcc ttccgcggtg aaacggcaga acttgcgacg
     3061 acggaaataa cgtgccatat ggctagtctc cagaatctat caattcaatc tgctcggcat
     3121 gcaaaaccat tttgctcagt ccgttctttg ccttgtggca tgaaatgaac ccctgaacgg
     3181 ttatgcgact gccgaccgtt atactgtgag taatggcctg gttttcgtgt ccgctaacaa
     3241 taacgggcat ttggcaccac gcctgccggt gaaaaccggc ttcctcctgc acagaacgat
     3301 gctcaagcac gaactggcag tgaggaattc ctgatggact gacctttcga aggggagtcc
     3361 tgcacacggt gccggacaac accagacggt tggtcatcag aaattactct tcagaatccc
     3421 cagcttcagc atcatcagcg gtttcgtttg cgaaatcatc gcgacgctca cggcgctcgt
     3481 ctttcgcttt aaccatcgga gatgcttcgg taacagcgtg cttggtacgc ataaccatgc
     3541 tgcggataac ggcatcgttg aagcggaagg tagtttccag ctcatcgatc acttcctgcg
     3601 gagcttcaac gttcatcaga acgtagtgtg ctttgtgcag tttgttgatc gggtaagcca
     3661 gctgacggcg gccccagtct tccagacggt ggatcttgcc ttctgcacca gtgatggcag
     3721 cagtgtagcg ctcgatcatg cccggaacct gttcgctctg gtcaggatgg accataaaaa
     3781 cgatttcgta atgacgcatc gaattgctcc ttacggatta ttcagcctcc tgtctgggtc
     3841 agccgaatcc cggggaggca aggaacgtgt taaaggtcgg ctgaaaaatg acgcgttata
     3901 gtacttgcat gccccgctaa actcaaggcc agccgacaaa taaatctcat ctttcgctca
     3961 aaagaactaa ctccctgatt tgaagttcat cacattccgt cacagtgttt tttcgaacaa
     4021 ctgcatcaga aatggtcacc atcaacttct ttatcattga ttaaaattaa atcagaagat
     4081 catcacagag gagagcacac aaagtcagcc gtcgaggagg tatctatgtt cagtcgagtt
     4141 ttagcccttc tggctgtgct tttgctaagt gcaaatacat gggcagccat tgaaattaat
     4201 aaccaccagg ccagaaacat ggatgatgtg caaagcttag gcgtgattta tatcaatcat
     4261 aatttcgcca ctgaaagcga agcgcgtcag gcattaaatg aagagacaga tgcgcagggc
     4321 gcaacgtact accacgtaat tctgatgcgg gaaccgggga gtaacggcaa tatgcacgcc
     4381 agcgcggata tttatcgcta gcaccaggta taaccaacga aacattgcca tagtttgctt
     4441 tgcccccttc gcagggggct tttttatacg gttattcacc gaattgcgac ttccggatct
     4501 ggctcatgag taatgcgatt acgtaagtcg cggcgcataa tctctattat ccagaaccag
     4561 aaaatatgcc ccaggatttc agacgcatat tcgtcaaaag gcaattgagt gagcggcggc
     4621 gttaaattca gcgccgggca aaatataccg tggactgcca ctgtcacaat aagccctgct
     4681 aatatcccct gccataattt cactttcgga aaaatttccg cgacaacaca ataccctatc
     4741 gcaaaaacca gtgagaagat gatatgcgtc accatcaccg ggttgataat atgttccgag
     4801 aatgtgtaca cagtttgggt tgggtcgata cctaaataat ctcgtagaaa gatatacggc
     4861 gggttaaatt catcgcgacc accggaaaat gtgcgcggtg gcaaggggac ttccgctccc
     4921 catttaacaa atgctgaaat aatgcctgaa agtacaccca caagtaacgc aacgccatag
     4981 cggcgacgct gcggatccgt tctgacaaac aatcctttga tagccataaa gcacctgatg
     5041 ttgatgattt tattaactgg agaggccccg gaatcagacc aattacatcc cgcaggaggt
     5101 aataattccg gggcgcactg cttacttctg cccgtaataa gcgttaggac cgtgtttacg
     5161 catgaagtgt ttattcatga ggtagctgtc gatgtgattc agttgtgggt taatgccgcg
     5221 ggcaatccac gccattttcg ccacttcttc catcaccacc gcgttatgca ccgcgtcgtg
     5281 ggcatctttc ccccaggcga acggtccgtg ctgatacacc acaattcccg gcgtatgcag
     5341 cggctcggcg ttgcccagcg tttcgataat cactttgcca gtgttcagtt catactcacc
     5401 ctgcacctct tcttcgctta atccacgcgt acacgggatg tcgccaaaga agtagtcggc
     5461 atgcgtagtg cctaacgccg ggatcgccag ccccgcctgc gcccatgcgg tagcatgagt
     5521 ggagtgggta tggacaatgc cgccaagcga cggataacga cggtagagtt caagatgcgt
     5581 cgcggtgtcg gaagatgggc gatactcccc ttccaccacc ttaccgctca tatcgaccac
     5641 caccatatcg tctgctttca tggtttcgta ggcgacgccg ctgggcttga tcaccaccag
     5701 cccgcgttcg cggtcgatag cgctgacgtt gccccaggta aaggtcacca gcccgtagcg
     5761 cggcagatcc atgttggctt caaatacctg ctgttttagc ttttgcatta tgccgcctcc
     5821 accatgcccg ctttcgccat gcgcgctttc acccaatcac gcgctttcgc cacttctgcc
     5881 gccgggtctt ccgccgtttc gctccacatc tcaatcaggt acggcccgca atagccactc
     5941 tgtttgagcg tttcgaaaca acgttcgaaa tccactacac cttcgccaaa cggcacgttt
     6001 ttgaagacgc caggtttggt gtctttcaca tgtaccgcga cgatatgccc gattccggcc
     6061 tgcaactcca tctgcacatc gttgtcccac gccgacaggt tgccgatatc cgggtagagc
     6121 tggaaccacg ggttgttgag atagtgcgcg taacccagcg ccttgctgat ggagttcatc
     6181 aacggataat ccatgatctc catcgccagc gtcacctgcg cgcggctcgc catctcaacg
     6241 ctctctttca ggccgtcacg gaaacgacga cgcgtttcgt tattggcttc ctgatagtaa
     6301 acgtcatagc ccgccagctg gatcacgcga atgccaacgt cctgggcgaa ctggatggct
     6361 ttacgcataa tctccagccc ctgcgcccgc accgcgtcat cttcgctgcc caacgggaaa
     6421 cgacgatggg cagaaaggca catggacggc acacgcacgc cggtttcaac aatcgcattc
     6481 accagcgcca gacgctgctc gcggctccag tcgaggcgcg agaggcgatc gtccgtttca
     6541 tccaccgaca tctcgacaaa atcgaagccc aacgtttttg ccagtcgcag gcgttccaac
     6601 cagcactccc cggcggggag cgctttttca tagatgccaa gcgggatttg tttggacaac
     6661 atatccgctc cttagcccca cagttcggcg atggaacgtt tgaactgacg tgcagcttca
     6721 accggagaag cggcatcacg gatgctgcga ccagcgataa acacatgaat cggaatacct
     6781 ttgaacagcg gcagatcttc cagcgccagg ccaccggtga cggtgacttt gaagcccata
     6841 tcggaaagac gtttgatcgc ggtaatgtcc gcttcgcccc acgctacgcc tgcggcctgc
     6901 gcgtcacggc tgcggtgata aaccacctgc tgaatgcctg cgtcacgcca ctgctgcgcc
     6961 tgttcccagg tccagtagcc ggtcaattcg atctgcacgt cgccgttaaa ctcttttgct
     7021 acgtccagcg cgcctttggc ggtgttgata tccgcacagc aaattaccgt cacccagtca
     7081 gcgttggctt cgaagcacat acgcgaaagg attttgcccg catcagcaat tttagcgtct
     7141 gccagtacga ttttgtgcgg gtagagcgct ttcaggtcac gaaccgcacg cacgccttcg
     7201 cccacgcaca gaatggtacc cacttcgata atgtcgactt cttcggcaat caggcgagtg
     7261 gtttcgtagg cgctatccat agtctggttg tccagcgcga cttgcaacat cggtaatgac
     7321 atcttcaatt ccttcttaag ctgccgcgtt ggtgcggtca atgagatcca gtacttcctg
     7381 ctcggtacgg caggcacgta aacggtcgaa attttcttca tcttcaaaca ggttgacgat
     7441 ctgcatgatg cccacttcct ggtgcgtgtt ggcatcgacc gccgccatgg tgatgaggat
     7501 atccaccggg tcgttatctt cgtggttgaa ctccagcggc tttttcagcg tcaccagcgc
     7561 aaaaccggtt ttcttaacgc cctcttccgg gcgaccgtgc ggcattgcca ggcctggtgc
     7621 gagcacaaag tacggaccgt gctgttcaac cgcatccaga atcgcctggt agtaacgcgg
     7681 ctcgacaaca tccgccgcca ccagcaggtc aacgccgatt tttactgcgt cctgccatgt
     7741 ctcagcttca gcctgcaggc ggatggattt attctccgcc agcgaatcac gtaatttcat
     7801 ggcgcgtcct tacttcacat cctgcgggaa atgcgctttg atcacttcca gcagtttcgg
     7861 gccaaagtcg gcaggagaga gcatgttgcg cacgccaacc acgtatttgt tgccggtcac
     7921 ggtgatttcg cccgcaatgt gcgtagaagc gatgatgata tccgcgccgc tcaactcgct
     7981 tttgtattcg ccaaccgcgc agctatttac cgtgtggtca atgtttgatt gggttaaaaa
     8041 ctggtccact ttcatcttca tgatcatgga actgccttgt ccgttgccac acacagccag
     8101 aatacgtacg gtcataatca aaactcctta ttaagcagac tgttctgcca gttgtttttc
     8161 tgcatcttct tctgcgcgca gtgcgcggcc agcgaagaac atataagcca gtgcaattac
     8221 aatgatgacg gccataaagg cgataccgat ggagaagaag ccctgcatca tcggcggtgc
     8281 cagaatcgac cagtccgcca tccccatcca ggcactcata ccggtaagtt tcaccgccca
     8341 gacgcaacca aagatttcga tcatccccat caccagacaa atcttcagcg ccgcacgcca
     8401 gccgccgaag tggttagcga acacgccgat ggtggcgttg gagaagaaca tcgggataaa
     8461 gcctgggata atcaggatcg aggaaccgca ggcgaccagg atgccaaccg caatcagctg
     8521 accgatggtg ccccacataa agccccagac cacggcgttc ggcgcgaagc tatagatagc
     8581 cgcacagtca atcgccagaa ccgcacctgg gatcaggcgc tgggaaatgc cgttaaatgc
     8641 ttcagagagt tccgccacaa acatgcgcac accctgagtg atgatgaaga tcgccaccgc
     8701 gaaggagaaa ccagtttgca ggatgtacac cgtccagttc actttacctg ccatcgcctg
     8761 cacggtgtcg ataccgaagg agagcagaat ggcaccaaag aagatggtca tcacaatcgc
     8821 cgtggagacg atgttgtcgt ggaaaatatt cagccagcct ggcagtttga ggtcttcaac
     8881 gctttcctct tttttgccga ggaacggcgc aactttatag gcaatccatg atgcaaactg
     8941 ctgctggtga ccaatggaga aaccgcagcc gtcggtgact tcctgagtcg gtttgtacat
     9001 catgttggag gtaatgcccc agtagagcga aaccagaatc gccgtacaga taatggtcgt
     9061 ccacatggaa tagccgaaaa taaacaacgt aacggcaatc agccccgcct gctggaacat
     9121 gatgtggccg gtcagcatga tggtgcgaat accggtaata cgacgcagca gcacgtaaca
     9181 aatgttcagc gccagtgcca gcagcaccgc gtaccctacc cagctatagg catcgcccat
     9241 gcggtcgatg gttgccatca tcgacgcgta agtatcggaa attgcgccgt taatgccgta
     9301 gacttcggac attttcgcca ccaccggttt gaaggtgctg gtgaggatgc cggaccctgc
     9361 ctgcaacaac atgaaaccaa ttatggtttt aatcgtgcct ttaataataa cgctgacact
     9421 tttgcgcagt aggatgtagc ccagacaggt cacaataccc agcaacaacg gggcattggt
     9481 catgacctgg ttaaaaaaca cggtaaagat gttgtagagg atctccataa cgatctcctg
     9541 aagaagaggt aaccgggtat tccacacacc cggaatgttg tgcactcact ctaattttca
     9601 aaagtaatca caacaagatt atttatgatt aaatgtgacg cgacccgcaa ataattccac
     9661 atggatagaa tgggattcac acaacgaaac aactattcat ttaaattcat ttagttaatt
     9721 gaattacaca ttccattaaa tctttccaca ctcaaaatca acaaccactt ccctggcaaa
     9781 aagtcaaaaa atggtcattg ccttcctgaa aaaatagtgt caggattaat caaaaccaat
     9841 catatattga ttcgaattga actatcatga aggtaaatgg cgatgagtaa agtgaaaagt
     9901 atcacccgag aatcctggat cctgagcact ttcccggagt ggggtagctg gctgaatgaa
     9961 gaaattgaac aagagcaggt cgcccctggc acatttgcga tgtggtggct aggctgcacc
    10021 gggatctggt tgaaatcaga aggtggcacc aacgtttgcg ttgatttctg gtgcggcact
    10081 ggcaaacaaa gtcacggtaa cccgttaatg aaacagggtc accagatgca gcgcatggct
    10141 ggcgtgaaaa aactgcagcc aaacctacgc accaccccgt ttgttcttga tccgtttgcg
    10201 attcgccaga tcgacgcggt actggcgact cacgatcaca acgatcatat cgacgttaac
    10261 gtcgctgctg ccgtgatgca gaactgtgct gatgacgtac cattcattgg accgaaaacc
    10321 tgcgttgatt tgtggattgg ctggggcgta ccgaaagagc gttgcatcgt ggtcaaaccg
    10381 ggcgatgttg taaaagtgaa agacattgaa attcatgcgc ttgacgcttt cgaccgtact
    10441 gcactgatca ccctgcctgc cgatcaaaaa gcggctggcg tactgccgga cggcatggac
    10501 gatcgcgcgg tgaactatct gttcaaaacg cctggcggct ccctgtatca cagcggcgac
    10561 tcccactact ctaactatta tgcgaagcac ggtaacgaac atcagatcga tgtggcgtta
    10621 ggatcgtatg gtgagaaccc gcgcggtatt accgacaaaa tgaccagcgc cgatatgctg
    10681 cgtatgggtg aagccctgaa tgcgaaagtg gttatcccgt tccaccacga catctggtca
    10741 aacttccagg ccgatccgca agagatccgc gtgctgtggg agatgaaaaa agatcgtctg
    10801 aagtatggct tcaagccgtt tatctggcag gttggcggca aatttacctg gccgctggat
    10861 aaagacaact tcgagtacca ctatccgcgc ggtttcgatg attgcttcac tattgaaccg
    10921 gatctgccgt tcaagtcatt cctgtaatct gtcgtttata ccggatagcg tggagaagtt
    10981 cgcgctatcc ggcgtagtat ttcctggcta tttcaaatat catctaaaaa aatcaaattt
    11041 tatcggaata gctcatgact gaagcacaaa gacatcaaat cctcctggaa atgctcgcac
    11101 aattgggctt tgtgaccgtt gagaaagtcg ttgagcgtct gggaatttcg cctgccactg
    11161 cgcgacgcga tatcaataaa cttgacgaaa gcggcaaact gaaaaaggtg cgcaatggcg
    11221 cagaagctat tacccaacag cgcccgcgct ggacgccgat gaatctgcat caggcgcaga
    11281 atcacgatga aaaagtacgt attgctaaag cggcctcgca actggttaat ccgggcgaaa
    11341 gcgtagtcat caactgcggc tccaccgcgt ttctgcttgg gcgggaaatg tgtggcaagc
    11401 cagtgcaaat catcactaat tatctaccgc tggcaaatta cctgatcgat caagaacatg
    11461 acagcgtgat cattatgggt ggacagtaca acaaaagtca gtccatcact ttaagcccgc
    11521 agggcagcga aaacagtctc tatgccgggc actggatgtt taccagcgga aaagggctga
    11581 ccgcagaagg gttgtataaa accgatatgc tgacagcaat ggcagagcag aagatgctga
    11641 gcgtggtagg gaaactggtg gtactggttg atagcagtaa gattggcgaa cgcgcgggaa
    11701 tgctttttag ccgtgccgat caaatcgata tgcttatcac cggcaaaaat gctaacccgg
    11761 aaatcctgca acaactggaa gcgcaagggg tcagcattct gcgagtttaa agatgctggc
    11821 ggaaaaatgt cacggcagca tctaacgcct caggcgtaat gcggtgacgc acgcctggct
    11881 gccatgaaca ggttagcagt ttatcccgtc ccgtctcgct taaggcctgc tgcaaacgta
    11941 gtgattcgtc ggcaggcaca acgtcatcgt cgaggccatg ccacagcagt agaggtctgt
    12001 ccccaagttg ttccaggtgg tttgtcgctt cccactctgc cagcggcgcg acaatgttat
    12061 tgaattcatt ctgctgtgct gccgtttcag gtatcagcgg tggaaacagt gaacgggcga
    12121 gcgatgtaaa atagcccgat cccatcatgc tggcggtaca tttcaccgtg gggtggcgag
    12181 cggtaattcc cagtgccgtc atcgcgccca tcgacgcgcc accgactgcc agacggtcat
    12241 caagcagcca gttttcttcg gctattgccg cacgtaaagt agtgaattcc tgcatacttt
    12301 gtagcaagat ttgccagaat tgatttaacc gccgcgcttc gtcgccacta aaacggctac
    12361 cgtgatcggg cgcatccggc atgatcaccc gcaaaccagc ctgcgccagc gcaacggcaa
    12421 aatagctata cactaggcag gatgaagtaa agccgtgata aaaaattacg cacggtaacg
    12481 gggtatcttt ttgcccgaca ggataagcat gaagaacggg aatatctgcc agctcgcgtg
    12541 attctatttc aatcatctgt ctctcctttt tgtctggtat gacatgaaga aatccttagc
    12601 acaagtttct gctggcggta ttagttcata atgttgagat gtgggttacg ctttcgtcac
    12661 gttttcattc gaaaatcgcg taccaggtaa caattcggga acattccccc aaagttaaat
    12721 taataaggca ctacactatg gttagcagga aacgtaatag cgttatttac cggtttgcca
    12781 gtttattatt ggtgttgatg ttaagtgcct gtagcgctct gcaaggtacg ccacagccag
    12841 caccgccagt aacgaaccat ccgcaagaga ttcgccgcga ccagacgcaa gggttacagc
    12901 gaataggtag cgtaagcacg atggttcggg gttctccgga tgacgcatta gcagaaatca
    12961 gagcaaaagc tgtcgctgca aaagctgatt attacgttgt cgtcatggtg gacgaaacca
    13021 tcgtgacagg acagtggtat tcacaagcca ttttgtaccg taaataaatc agacaactta
    13081 catagtcagc tttacattgc tttgcgtccc tatgcgttta cctgtgcaca ataaattacc
    13141 ggctggaagc ctgggcgttt attaccgcga tggaatgccc tgcaacatgt ggggaaacga
    13201 aaaatggagc tgacgatgaa acaattactt gcctcaccct cgctgcaatt agtgacttat
    13261 cctgcgagcg ccacggcaca gtctgcagaa ttcgccagtg ctgactgcgt gacgggcctg
    13321 aatgaaatag gtcagatctc agttagcaat atttcagggg atccgcagga cgtggaacgt
    13381 atcgtggcgt taaaggccga tgaacaaggt gcgtcatggt accgcattat taccatgtac
    13441 gaagaccagc agcctgataa ctggcgcgta caggcgatac tgtatgcatg agtgttatat
    13501 acagcaaatg aacaatgccg gatgcggcgc gagcgccttg tccggcctac aattttcgca
    13561 tatttcattg agttaatcga aaccagcgtc gcatcagtcg tatacgctta cacacacact
    13621 ccccccgtcg cccgcagcaa taaatcattc tggatctgct ctgacaaccg tacgccgccg
    13681 cgcgtatcta acatcacctg acaccacgcc tgcgccattg gcggagaggc atatttcaac
    13741 atttgcgcac cgcagcccag caggaatagc tgatgagtaa tctctcgccc cagttcttca
    13801 gctggcttgc gcagctgctg ctgtaaacga cgaaccgcgc gatcaaaata gcgatcctgc
    13861 cctttcactt ccacaaacgc ttccgacaat aagtcgtata cgcccgcttg cttattgaga
    13921 acgcgcagca catccaggca cataatattg ccggaacctt cccaaatact gtttaccggc
    13981 atctcccggt aaagccgcgg cagctcgctc tcctcgcaat aaccaatgcc gcccagcacc
    14041 tccatcgctt cggccacaaa cggcatacct cttttgcaga tcacaaattt tgccgcaggc
    14101 gtaaataaac gcgcccacag ggcttctttg gcatcggcac gccggtccca cgctcgcgca
    14161 agacgaaaca gcaacgccgt ttgcccttca agctgaagtg ccatgcgact taagacatga
    14221 cgcataaggg gctgttggat caatggatta ccaaaaacat ggcgttgatg tgcatgataa
    14281 atcgccagcg aaaatgcacg gcgcatcatg gcatggctac ccagggcgca atcaaaacgc
    14341 gtcatcccac ccattttcag gatcagacga attccttccc cttcctgccc caacaaccag
    14401 ccaatggcat cctgaaactc cacttcgcag ctggcgttag agcgattacc cagcttatct
    14461 ttcagccgct cgaggcgaat cgcgttgcgt tgcccgtcag gcaaaaagcg cggcacaaaa
    14521 aagcaggaca aaccgcccgt agtctgcgcc agcaccagat gcgcatcgct ttgcggcacc
    14581 gagaaaaacc atttatgccc caccagccga taagagccat cttccaggcg ctctgcgcgg
    14641 gtggtgttgc tcatgacatc ggaaccgccc tgcttttccg tcattcccat gccaatcaac
    14701 aaaccgcgtt tttgcccacc tggcaataag tgagaatcgt agcgatcgct cagcagcggc
    14761 gtggtccagt cctgaaacgg cgtgggtaac atctgtaaca acaatggcgt ggcggcaaag
    14821 gtcatcgtta ttggacataa cgacccagcc tcaacctgcg catgtaacat aaaacgcgcc
    14881 gcgcgcgcca caaatgcgcc ggagcgagcg tcttcttccc aggcaagatt gtgcacccga
    14941 ttggtacata acgcctgcat cagcaggtgc caggcggggt gaaaacgcac atcgtccaga
    15001 cggcgtcctt gcgcatcgta gcgcaataat tcaggcggat tcacattcgc cagccgcccc
    15061 agttcaagag attcagccgt tcctaactgc tgaccaatac tggctagaaa atcgctatcc
    15121 cagccagcac cttcacgcgt taccgcttca cagagcgcgc catcagacag gtataagttg
    15181 ctgttattta atggtatagg ttgattaaaa acggtgtgag tttgccagtg cactgtgtct
    15241 ccctccatca atggcagtca cccttagtat ggtcactgcc atggattcct gacagaaaga
    15301 gattgctaaa acattcttat ccagcaatgt aatgcggaac gaaacgtgag gtgtctttgg
    15361 tgatcagcgt gttatcttca cggatcccca ttccgcacgc ttcatcatcg acaatccagc
    15421 taccgatgag tgtgtagcta tcgccaaatc gcggcagagg ttgaaacgcc tggtagatca
    15481 tcggttcatc ggcgtaatca ccatcagcgt ggtcaacgac gttattctga ccgtcgaaaa
    15541 tggtgacgtt tccgccttcg cgcgagtaga tcggtttacg cacatagctt tcgccagcgg
    15601 caatctgcgg tttttcgccc tcaaaccagg atgcaagaag gttaggatga ccagggaaga
    15661 agcgccaaag cagcggcatt agccctttat tactcaagat acttttccat aacggctcca
    15721 cccattgctc gcgacgcttg cgcagcagcg gaccgttatc gtcacgcatc atccactcca
    15781 gcggatacag cttaaatgca cgctggatga cattatcatc aagatcggtc agtacaccgc
    15841 cgacgcccaa accgagatct tcaatgtaga taaaccgcga ctcctgccct gcctgctggg
    15901 cgcagtcctg caagtacagc acggtactcc tgtcttcatc ggtgtcctga cagcagcaaa
    15961 aataaaacgg ttcccgactg taaagctcgc taaagcgtga aatcaggcgt tcctgaatag
    16021 cattgtactg atcggcatca cacggaataa tgccgctgcg ccgggcatcc tccagccaca
    16081 gccactggaa ataagccgac tcgtacaatg aagttggcgt atcggcgttg tactccagca
    16141 gcttcaccgg cgcattgcca caccaggcaa aatccatccg tccatacagc gaaggatcgc
    16201 gggcgcgcca gctttcagcg atcacatccc agtacaacgg cgggatcgcc agttgcgtca
    16261 ggatctcttc gtctttaacc gctcgatcca ccacctcaag gcacatctga tgcagttccg
    16321 cagtcggttt ttcgatctgc tcttcaatct ggcgcagagt aaaacggtaa gcccgactct
    16381 catcccaata gatttcattg tcgatgatat gaaagtcgaa accgttgtca gcggcgatct
    16441 ggtccagatc ccgccgcaca ggaacgttgt gtcttagcat gattagcctc cccaatgccc
    16501 acgggcgctg gaagaacgac catagccgcc gcgagaaacg gttgacgctt ttttggtggt
    16561 aaagccgccc gaagagtaag actctttttt gccagaaccg gagcgccagg agtaatcacc
    16621 ggaagtgttg cgccagacgg ggcgggaagc aaatgaggat ccgccgctgt tataaacaaa
    16681 ctgctcatcg cgatctttac gaataacccg gcttaacaaa aatccagata ttaccgggat
    16741 ccaactctgt tcaacgttgt cgtagtaaca attttcatac ttagactgac agttctgctg
    16801 agtcatattc ttcggaacat cggcataaaa cgccgctttg gcgttgttcc agccacgcgc
    16861 gcaaatgtcg gcattattac cgtcatcaat acaatcctgc actgtcgaat aaaacgttcc
    16921 gtcgccgtcg ttatcgacat cgccactatc gccacaacct ttcaatacaa aaaaagcggc
    16981 accgcccatt agagccaacg ttaagtattt ttgtgcgtag cgattgcgac gcggttcaaa
    17041 aggattattc ggtctaccaa tgcgactgat cgctccgtga ccaattttac tgttatttct
    17101 tgatttgcgt tttctggcca tgatcgtcca ttaccatgtc atacaagctg cgttaaaaat
    17161 accgccagcc agcgcggcgg ttcccataaa cattcctgct gcggtattgt gattaataat
    17221 tttttcgctt aatgcgggca tataaagtct gacaccagca aaaacgagta actgaatcac
    17281 cagtgcaatc ccgccccagg cgaaatagtc tggaatactc accgcattga ttgccgcact
    17341 ggataatggg ataacgtaac ctaacaatgt accactgaac gccagtgaag ccgcggtatt
    17401 attgtttttg attaactgcc attcgttgtg cggtgtaatt ttagaataga taaaaagaaa
    17461 aataatcacc atagccacgc caataaaaaa ataggcgcta aaggcaagaa gtgaatccag
    17521 tatatgcatt gtaaacgtcc ttgtaattaa ccaataatat gaagtgatgt cagcgggata
    17581 tcgacaccca acgcgcgcga aaataaccac tctggctcgc cgagatcgtt aaaagattcc
    17641 tcaccattta acagcaaata ttcgtaagta tcttcggtta cttgccgctg gtagcccatg
    17701 gtaaaattat ggacctccca tttggcatgg ttttgatttt ctactttttc cagcatgtag
    17761 acgggttcga tatttcccgg ttcttcgctg ttaaaaaatc gctgccagcg tttttcctgc
    17821 cagtttaaga tcattgcccc catcactttg gcgttgatcg cctcgcgcca gtgactctct
    17881 ttactgatac cgtagctctc ttcatagaca aagagcttga tatcatcaat gtcatcaata
    17941 tcttcgccgc cggtggtatt gatttgcaga aattcatcgc ctgaagtgta gtagcggaaa
    18001 atctgactac cgccgcccag atcgatgtgg ctgacagcgg ctaccgtaaa ttcctcaccc
    18061 ggcagcgcaa tcagcaattc atcttccagc aaacgaaacg ctaacgtgtc gagcgtaaag
    18121 ccactattga gatgaagtcc cagcggaccg cgagcgatag caggcttatt atccttgcca
    18181 aacagacgct ggaaaaaacc agacatattt tatccttaaa atagttgcca gccttttagc
    18241 gaggctggca aaaaaattac tcgccctgtt ggcgttgcag tcttgctaat acatcctggg
    18301 cgctactttt attgctaccg ccaattccgg cttccgccag cttttcatca agatcgcgac
    18361 cgtctgcgac tttttccaac tgtgcagcag catccaggcg agcctgacgt tcggcctgac
    18421 gcgtttgcag gcgttttaag gattctgccg ctgtcgaaac gctggaagat gcgccaacgg
    18481 tagaggttgt tacagcctgt tgtgcacgct gcatggcttc agtggcttta acgacttcca
    18541 tttgctgctc aaactgagcg atacgctgcg ctgtcgctgt aactgctttt tccacgccat
    18601 cacgggagac ttccagattc gacaacactt gctcttcagc ggtaatgaga ttctcaaggc
    18661 gcgcgatttc ttcagcaact tcgttaatca acgacggatt aacgttcttg cttaacgctt
    18721 ccagcgcacg ggcttccaga ctggctttgc gctcacgtaa atctttcagc ttatcgtgac
    18781 ttaatttcac ccgcgccagc agatcaacgc gagattttcc ggctttatcg agttcagctt
    18841 tagcgtcacg aatatgctgt tccagcatgc gcacgccctg ggtttcttca atggattctt
    18901 ccgcctggga gataaacgat ttccccagcg taaataaact ttttaaaatt cccataaata
    18961 cccgtccttg taattagtgt gaatattctt ctgtaatttc agccagatcc aatgcgttat
    19021 ctaccagcga ggttatctca agcaggatat cttcaagaga agatttaagc gataacgccc
    19081 cgaaaacaat gtaatactct tcctgttgca cgctggaga
//
LOCUS       DAZGXZ010000098        18539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000098 DAZGXZ010000000
VERSION     DAZGXZ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.098"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..584)
                     /gene="rsmG"
                     /locus_tag="ACHKJF_003592"
     CDS             complement(<1..584)
                     /gene="rsmG"
                     /locus_tag="ACHKJF_003592"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP0950886.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDG"
     gene            complement(648..2537)
                     /gene="mnmG"
                     /locus_tag="ACHKJF_003593"
     CDS             complement(648..2537)
                     /gene="mnmG"
                     /locus_tag="ACHKJF_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP0950887.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2915..3358)
                     /gene="mioC"
                     /locus_tag="ACHKJF_003594"
     CDS             complement(2915..3358)
                     /gene="mioC"
                     /locus_tag="ACHKJF_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP0950888.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3448..3906)
                     /gene="asnC"
                     /locus_tag="ACHKJF_003595"
     CDS             complement(3448..3906)
                     /gene="asnC"
                     /locus_tag="ACHKJF_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP0950889.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4058..5050
                     /gene="asnA"
                     /locus_tag="ACHKJF_003596"
     CDS             4058..5050
                     /gene="asnA"
                     /locus_tag="ACHKJF_003596"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP0950890.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(5055..6506)
                     /gene="viaA"
                     /locus_tag="ACHKJF_003597"
                     /pseudo
     CDS             complement(5055..6506)
                     /gene="viaA"
                     /locus_tag="ACHKJF_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
     gene            complement(6500..7996)
                     /gene="ravA"
                     /locus_tag="ACHKJF_003598"
     CDS             complement(6500..7996)
                     /gene="ravA"
                     /locus_tag="ACHKJF_003598"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP0950891.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8219..10087
                     /gene="kup"
                     /locus_tag="ACHKJF_003599"
     CDS             8219..10087
                     /gene="kup"
                     /locus_tag="ACHKJF_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP0950892.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10254..10673
                     /gene="rbsD"
                     /locus_tag="ACHKJF_003600"
     CDS             10254..10673
                     /gene="rbsD"
                     /locus_tag="ACHKJF_003600"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP0950893.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10681..10818
                     /locus_tag="ACHKJF_003601"
     CDS             10681..10818
                     /locus_tag="ACHKJF_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004171564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0950894.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGETARVNP
                     P"
     gene            10782..12185
                     /gene="rbsA"
                     /locus_tag="ACHKJF_003602"
                     /pseudo
     CDS             10782..12185
                     /gene="rbsA"
                     /locus_tag="ACHKJF_003602"
                     /inference="COORDINATES: protein motif:HMM:NF008030.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            12190..13155
                     /gene="rbsC"
                     /locus_tag="ACHKJF_003603"
     CDS             12190..13155
                     /gene="rbsC"
                     /locus_tag="ACHKJF_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP0950895.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFCIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13180..14070
                     /gene="rbsB"
                     /locus_tag="ACHKJF_003604"
     CDS             13180..14070
                     /gene="rbsB"
                     /locus_tag="ACHKJF_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP0950896.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14196..15125
                     /gene="rbsK"
                     /locus_tag="ACHKJF_003605"
     CDS             14196..15125
                     /gene="rbsK"
                     /locus_tag="ACHKJF_003605"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0950897.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15129..16121
                     /gene="rbsR"
                     /locus_tag="ACHKJF_003606"
     CDS             15129..16121
                     /gene="rbsR"
                     /locus_tag="ACHKJF_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP0950898.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16087..17514)
                     /gene="mdtD"
                     /locus_tag="ACHKJF_003607"
     CDS             complement(16087..17514)
                     /gene="mdtD"
                     /locus_tag="ACHKJF_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP0950899.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17537..18229)
                     /gene="yieP"
                     /locus_tag="ACHKJF_003608"
     CDS             complement(17537..18229)
                     /gene="yieP"
                     /locus_tag="ACHKJF_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP0950900.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4472 a         4597 c         4838 g         4632 t
ORIGIN      
        1 ccatccaggg ctggaacctg aagtttaacc actgattcga cctgatattc ttcgggcaac
       61 aaagcgattt catcttccgg catttgccct ttcagcgcgt agaaacggcc ttgctcacca
      121 ggaagatggt ggcaccagct caccatatcg ttcagagagg caaaagcgcg gctaattacg
      181 ccatcaaatg gcggctctga aggaaactct tctaccctgc tctgtactgg ttcaatattc
      241 tccagtttaa gctcatgttg cacctgacga aggaaacgca cgcgtttacc aaggctatcc
      301 aacagagtga aatgggcttc aggacgcacg atagagagtg gaatgcctgg cagtcctggt
      361 ccggtgccga catcgataaa ccgttcacct tgcagatacg gtgccaccac aatgctatcg
      421 agaatatggc gtaccagcat ctcattagga tcgcggaccg aagtcaggtt gtacgctttg
      481 ttccatttat gcagcatatt cacgtaggca ataagctggt ttttctggtg atcggtaagc
      541 gaaatacctg cgtctttcag cagtaaggag agtttgttga gcacggtgat tacctgttct
      601 tgatgcgttg cctggtaagc gggtgcttac caggcatttt taatgcgtta tgcgctacga
      661 cgcagcatac cctgtttttt cagccacacc agcagaatgg agatggccgc aggcgtgacg
      721 ccagaaatac gcgaagcctg gccgatagag gctggtttgt gatcgttaag tttggcgatc
      781 acttcgttag aaagaccgga tacctggcgg taatccagtg tcgcgggtag cagggtgttc
      841 tcgttacgca gctgcttttc gatctcatct tgctggcgcg cgatataacc ttcgtattta
      901 acctgaatct caacctgttc cgccgcctgt tcgtctgtca acgcaggggc aaacggcgtc
      961 agcgtggtta atttttcata agtcatttcc ggacgacgca gcagatcttc accactggct
     1021 tcacgggaaa gcggcgcagt caggtgagca ttcacttcgg ctgcagcttc cgccgacggg
     1081 gttacccagg tcgatttcag acgctgacgc tcacgctcga tattctcaag tttctcgtta
     1141 aagcgcgccc aacgttcgtc atccaccagg cccagttcac gaccgatttc agtcaaacgc
     1201 agatccgcat tatcttcgcg tagcatcaga cgatattctg cgcgcgaagt aaacatacga
     1261 tacggttctt tggttcctaa agtgcacagg tcatcaacta gtacgccgag atacgcctga
     1321 gaacgtgccg gagcccaacc ttctttgtca gcagacagac gggcagcgtt aagaccggcc
     1381 agcaaacctt gcgcagcggc ttcttcgtaa ccggtagtgc cgttaatctg accagcaaag
     1441 aacagcccct ggataaactt gctctccagc gtcggtttca ggtcgcgagg atcgaagaag
     1501 tcatactcaa tggcataacc cggacgcacg atcttcgcgt tttccatccc ctgcatagag
     1561 cggacgattt gcatctgcac atcgaacggc aggctggtgg agataccgtt cggataaatt
     1621 tcattagagg tcagtccttc cggttcaagg aagatctgat gctgatttct gtcggcgaag
     1681 cgcataactt tgtcttcgat cgacgggcag tagcgtgggc cgacaccttc gatcacccct
     1741 gcatacattg ggctacgatc gaggttactg cggatcacat catgggtttt ctcgttggta
     1801 tgagtgatat aacacggcac ctgctgggga tgctgggacg cattgcccat aaacgagaat
     1861 accggcattg ggttatcgcc atgctgttgc gccagcacac taaagtcgat agttcgagca
     1921 tcaatacgcg gtggtgtccc ggttttcaga cgaccaacgc gcagcggcag ttcacgcaaa
     1981 cggcgagaaa gcggaatgga cggcggatca ccagcacggc caccgctgta attatccaga
     2041 ccgatatgaa ttttaccgtc gaggaacgtc ccaacggtga gcacgacggc tttggcacgg
     2101 aacttcagtc ccatttgggt aacagcaccg accacgcgat cgttttcgac aataagatct
     2161 tcaaccgcct gctggaagat catcaggttc ggttggttct ccagcgccgt acgtaccgcc
     2221 tgacggtaga gcacacgatc cgcctgagct cgggtagcgc gaaccgccgg tcctttgctt
     2281 gcgtttagta tcctaaactg gatacccgct tgatcgatcg ctttcgccat cagaccgccg
     2341 agtgcatcca cttcttttac cagatgtccc ttcccaatac cgccgatcgc cgggttgcag
     2401 ctcatctgcc ccagagtgtc gatattgtgt gtcaaaagca gagtctgttg acccatacgc
     2461 gccgcggcca tcgcggcctc ggtgcctgca tgacccccgc caatgatgat gacgtcaaaa
     2521 ggatccggat aaaacatggt gattgcctcg cataacgcgg tatgaaaatg gattgaagcc
     2581 cgggccgtgg attctactca actttgtcgg cttgagaaag acctgggatc ctgggtatta
     2641 aaaagaagat ctatttattt agagatctgt tctattgtga tctcttatta ggatcgcact
     2701 ccctgtggat aacaaggatc cggcttttaa gatcaacaac ctggaaagga tcattaactg
     2761 tgaatgatcg gtgatcctgg accgtataag ctgggatcag aatgaggggt tatacacaac
     2821 tcaaaaactg aacaaccgtt gttccttgga taactaccgg ttgatccaag cttcctgaca
     2881 gagttatcca cagtagatcg cacgatctgt atacttattt gagtaaatta atccacgatc
     2941 ccagccattc ttctgccgga tcttccggaa tgtcgtgatc aagaatgttg atcttcagtg
     3001 tttcgcctgt ctgttttgca ccggaatttt tgagttctgc ctcgagttta tcgatagccc
     3061 cacaaaaggt gtcatattca cgactaccaa taccgattgc gccaaagcgg accgcagaaa
     3121 gatcgggctt ctgttcctgc aatgcttcat agaaaggaga aaggttgtcc ggaatatctc
     3181 cggcaccgtg ggtggagctg ataatcagcc agatccccga ggcagataaa tcttccaaca
     3241 gcggaccgtg cagcgtttcg gtggtaaaac ccgcctcttc cagcttttca gccaggtgtt
     3301 ctgctacata ttcggcaccg ccgagggtgc tgccgctgat aagagtgata tctgccataa
     3361 accgccacct ttattaagag tggcgtattc tacgctgtga acgcgttggg atctacctgt
     3421 ggaaaagtat gggattaaaa aagccgatta tggcttgatg gtacgcatga tcgggttttg
     3481 caggacgatc aatgtctcgg tggactgaat ttcatcaatt gtttggatct tgttgataag
     3541 tacatgctgg agagcgtcga tcgaacggca catcactttt ataaagatgc tgtagtggcc
     3601 ggttgtgtaa taggcttcag tgacttcatc aaggctttcc agctttgcca gcgcggaagg
     3661 gtagtctttg gcgctcttta atataatgcc gataaagcag cctacgtcat aaccgagctg
     3721 cttcgggctg acatcaatac gcgccccggt aatgatcccc gcctgcttca ttttctctac
     3781 tcgaacgtga atcgtccccg gactgacgcc aaattgtttc gccagttcgg cgtaagcggt
     3841 gcgcgcattg cccattaatg cttccaggat gccacggtcc agattgtcga tcagataatt
     3901 ttccatagga ttttcttatg cagattgatg attcattcta ttttagcctt cttttttaat
     3961 gaatcaaaag tgtgttaggc tttttattga atgattattg catgtgtgtc ggtttttgtt
     4021 gcttaatcat aagcaacagg acgcaggagt ataaaaaatg aaaaccgctt acattgccaa
     4081 acaacgccaa attagcttcg tgaaatctca cttttctcgc caactggaag aacgtctggg
     4141 gctgatcgaa gtccaggcgc cgattcttag ccgtgtgggg gatggcacgc aggataactt
     4201 gtcgggctgt gaaaaagcgg tgcaggtaaa agtgaaagct ctgcctgatg cccagttcga
     4261 agtggttcat tcactggcga agtggaaacg tcagacctta gggcaacacg acttcagcgc
     4321 gggcgaaggg ctgtacacgc acatgaaagc ccttcgcccc gatgaagagc gtctttctcc
     4381 gttgcactcg gtctatgttg accagtggga ctgggaacgc gtaatgggcg acggtgagcg
     4441 tcaattctcg actctgaaaa gcacggtaga ggcgatctgg gcgggaatta aagcaaccga
     4501 agctgcagtt agcgaagagt ttggtttggc accgttcctg ccggatcaga tccacttcgt
     4561 acacagccag gagttactgt ctcgttatcc ggatctagat gccaaagggc gtgagcgggc
     4621 gattgcgaaa gatcttggcg cggtattcct cgtcgggatt ggcggcaagc tgagtgatgg
     4681 tcatcgtcac gacgtgcgcg caccggatta tgatgactgg agcaccccgt cagagctggg
     4741 ccatgcgggt ctgaacggcg atattctggt atggaaccct gtactggaag atgcgtttga
     4801 gctttcttcc atggggatcc gcgtggatgc cgacacgttg aagcatcagt tggcgctgac
     4861 cggtgatgaa gatcgtctgc agctggaatg gcatcaagcg ctgctgcgcg gtgaaatgcc
     4921 gcagaccatc ggcggcggta tcggccagtc tcgtttgacc atgctgctgc tgcaactgcc
     4981 gcatatcggc caggttcagt gtggagtatg gccagctgct gttcgcgaga gcgtcccttc
     5041 tctgctgtaa taatttatcg ccgccagcgt ctgagcaggc ggcttcgcat cccggtatca
     5101 aagcgccaga tatgatcgaa aatgcgcatg atgccgggtt tgccgtgtgc cgacatcgcc
     5161 acggcatgaa agcgatgctg atgtacccgc tgcagctctt tcactttact cgtcacgtcg
     5221 tcaggcaacc gctgagcgat aaaatcagaa atcaccaccg catcggcatc aaaccattcc
     5281 ctgctttgca agcgttccat aatggcgcga aaacaactgg caagatcggt gccgccacga
     5341 aactgctggc ttaaaaaacg gattgcttgt tcgatgcctt gtgggcctga aagctcataa
     5401 cggacgatct cggtggaaaa tagcataata tagcagcgcc ggttttcagc gagagcaatg
     5461 cgcatcaagg ccaggcagaa cgctttcgca cactgttcat taaagccgcc cattgagccg
     5521 gaagtatcca ctcagacaat aaacggcccg cgcggctgtt catcgtaatc tttatgtacc
     5581 accggacgtt cgatcacttt ttcacgccac gactcaccgt gcaggcgata ggtgagcaac
     5641 tgtttttcca ccagccgacg gtaaaactca tactccagtt ccgttatccc tagtgtcgcc
     5701 agttctggcg gcagaagacg taaaatatca tcgctttgtt gcagaccatc aacctgctca
     5761 ggaaccgtcg ccggttcgcg caccatggtg cggaaggttt ccatctgcgc atcgttgcgc
     5821 ggtattgatt tggcttcccg agaacgcccc agctgctctg ccaggcgttt cagttccggc
     5881 tgttcgttaa gaaattcacc gtatttcaca atcaactgat agtcgccacg tttaagctga
     5941 ccggcgctca tatcccacag acgaccagct gcagtattgt tatctgcgag aatcggttca
     6001 agttgtccgc tcagcgtcat gcgttcctga acttcactca acagttgttc gcgttcttct
     6061 tctaatagct gttgatttaa cgtcgttgct tgctcgatca gacttaaacg ccaacgctgg
     6121 agaaaaagcg tgtgtaacgc tgaagtgatc gtgctgttag catcaaccaa ctgtcgggct
     6181 tgttctgccc atggataatt cagacgatgc agtaagtcca ggatctgtgg tagctgcaca
     6241 ataaactgtg gcgtggagag gagctggctt tgctgatagc acatcacctc ttcggtgagt
     6301 tccggcggga ctcgggcatc tttcagccga ctgcgcagcg cttcacgcca gcggggaaca
     6361 tcatcagtga ttgccgcctt caggcgtggg aatttttcaa agaagaccgc cagctgcggt
     6421 gaggccagca gtgcgatgat catctcttcg atcaatccct cttcgctgac ggccagcatc
     6481 acattaagcg tatccagcgt tagcattgtt gtgcctggcg aatctgtgca ccgacatcct
     6541 gtaggctggc ttcaatgcga cctaaccagt cgccagggat aaacagacat ttttgctgtt
     6601 cgctgaataa agcgtgttgc ttgcgccagt cgctttcaag ctcttccagt tgttgtttta
     6661 tttcaccagg cagaccttca gccgatgaac cgggaagtgc cagcgtactg ccttgtaaac
     6721 ttacatcgcg aacgacaaga tgttgggcgc tatcaacttc cagattcagt ttctgggcaa
     6781 agccgatacc gttcagttta ccgcgaattt caccaccttt gctcagccac tgttccagcg
     6841 cgctacgctc aaaggagata tgaaccactt ccatatcatg cagttttaac ggtttttgca
     6901 gcagtagagt cagagtggaa gcagtaacgt taacaggaag ttgatactgc tgacgacggc
     6961 tgaaaatgcc gcccagacga attaccgtta aggctgtttt atcgctttgt tgctgctgga
     7021 gttgcaggtg acgttgcaca atcgcgccaa ggcgagtcaa catcccttgc tgttgccagg
     7081 cgtgaccggt catcaataca tcaatttgtt gttgtatcaa attcaggctt tgcgcgtcat
     7141 accacaggca atctttcagc aaaatgagat ctaccggggc aacagcactg cgaccgctaa
     7201 aaaaggcgct ggcctgcaat aatcggatcg cttttttcca gcgacgatcc gagacataag
     7261 gcgcatccgg taatttatcc agttgctggc gcagcataaa aatcagctca aatacatgat
     7321 cgggcagcgt aatttcacca atctctttct gccagcgttc atattcttca tctgtgacct
     7381 gcaaggcatc aggaaccgga ttgtcgtttt catcctgttg actggtcagc atggagcgga
     7441 aattcgcttt atcctgcact ttatctaacc acagacgaat cagcatgcgg tcatataacg
     7501 cttccagact gctgtctgct tccggcagct cgttggaggc cgccaccagc agacgcatcg
     7561 ggattttttc tacgtgtgca ccgttgcgga actggcgctc gttaatggcg gtgagcaagg
     7621 tattaagaat tgccgggccc gctttccaga tctcatccag aaagacaatt tctgcttccg
     7681 gcaggtaacc gctggttaaa cgttcatagc gcccttcatc ttttagcgcc tgaatagaaa
     7741 ggggaccaaa aacttcttcc ggcgtggaga agcgggtcat cagatattca aacgcgcggg
     7801 cattctgaaa ggcgaatttt aagcgccggg cgatcaaact tttggcaata cctggcgggc
     7861 caaggaggaa cacactttca ccacttaatg ccgctaataa acacaagcgg atggcgtggc
     7921 tacgttcata aagccccttt tccaacgaac tgctcaggcg ggaaattctt tccgctaata
     7981 aatgagggtg agccataatg aagtggcgtc ctttcgtcaa aagttctgcg taaattgcga
     8041 gtatagacgt ttcttgctgg tggctaaaat agtctcaaag ggggggtatt tttctttgag
     8101 ccaggttaat gtggccgcat ttaggagtac gattttgccg ttaatcgtgc atactgtgcg
     8161 cttttttgtg ggccaaggga ctaagcacac atttcatatt tcaacgaaag actagtctat
     8221 gagcactgat aataagcaat cattgcccgc gattaccctc gcggcgattg gagttgtcta
     8281 cggcgatatt ggtaccagcc cgttatatac acttcgtgaa tgtttgtccg gccagtttgg
     8341 ttttggcgtt gaacgcgatg ccgtgtttgg ctttttatcg ctgatcttct ggctgctaat
     8401 ctttgtggtt tccattaaat atctcacctt cgtgatgcgg gcagataacg ccggtgaggg
     8461 ggggatcctg acgttgatgt cgcttgccgg gcgtaatacg tcggcgcgaa ccacatcaat
     8521 gctggtgatt atggggctaa tcggcggcag ctttttctat ggtgaagtcg tcataacacc
     8581 cgctatttcg gtgatgtcag ccattgaagg tctggaaatc gtcgccccgc agctggatac
     8641 ctggatagtt cccctctcaa ttatcgttct cactttatta tttatgattc aaaaacatgg
     8701 cacggctatg gtcggtaagc tgtttgcgcc gatcatgctg acctggtttt tgattctggc
     8761 aggactgggg ttacgtagca ttattgctaa cccggaagtg ctgcatgcgc tgaatccaat
     8821 gtgggcggtg catttcttcc ttgaatacaa aacggtttct tttattgcat taggggcagt
     8881 ggtgttgtcg attacggggg tcgaggcgct gtatgctgat atggggcact ttggtaagtt
     8941 ccctattcgc ctggcgtggt tcaccgtcgt attaccttcc ttgaccctta attacttcgg
     9001 tcagggagcg ctgttgttaa agaacccgga agcgattaag aacccgttct tcctgttggc
     9061 accggactgg gcgctgatcc cgctgctgat catcgccgca ctggcgacgg taattgcctc
     9121 gcaggcggtt atctctggcg tcttctcatt gacgcgtcag gcggtacgtc tgggatattt
     9181 atcgccgatg cgcattattc acacctccga aatggagtca gggcaaatct atattccgtt
     9241 tgtgaactgg atgctctatg tcgcggtcgt gattgtgatt gtcagctttg agcactccag
     9301 caacctggcg gcggcgtacg ggattgcggt gaccggaacc atggtgctga cgtctattct
     9361 ctcgactacc gtggcacgtc agaactggca ctggaataag tattttgttg cgctgatcct
     9421 gattgctttc ctttgtgtcg atattccatt gttcaccgct aacctcgata aactgctctc
     9481 cggcggctgg ttgccattga gcctcggtac ggtgatgttt atcgtgatga ccacctggaa
     9541 gagcgagcgt ttccgcttgc tgcggcggat gcatgaacat ggtaactctc tggaagcgat
     9601 gattgcttcg ctggagaaat caccgcctgt tcgcgtgccc gggaccgcgg tgtatatgtc
     9661 gcgtgcaatc aacgtcattc cctttgcgct gatgcataac cttaaacata acaaggtatt
     9721 gcatgagcgg gtgattctgt taactctgcg taccgaagac gcgccatatg tccataatgt
     9781 ccgtcgggta cagattgaac aactgtcgcc cactttctgg cgcgtggtgg caagttatgg
     9841 ttggcgagaa acgccaaacg tggaagaagt tttccaccgc tgcggtctgg aaggattaag
     9901 ttgccggatg atggaaacct ccttctttat gtcgcatgag tcgttgatcc tcggcaaacg
     9961 cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta atgcgctgcg
    10021 tgcaccagat caatttgaaa ttccgccaaa cagggttatc gaactgggta ctcaggtcga
    10081 aatctaacgc cagacgcctc ctttctccat aagggggcgt ttttgttttc atggttaatc
    10141 accatgtaaa acgtttcgag gttgatcaca tttccgtaac gtcacgatgg ttttcccaac
    10201 tcagtcagga ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa aaaatgaaaa
    10261 aaggcaccgt tcttaattct gatatttcat cgatgatctc ccgtctggga cataccgata
    10321 cgctggtggt gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt atcgatatgg
    10381 cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgtcaca aatgaaatgc
    10441 aggtcgaggc tgccattatc gcggaagaga tcaaacaaca taatccgcaa ctccacgaaa
    10501 cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt gaaattcgtt
    10561 acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta attcgcagcg
    10621 gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc tgaggccgtc
    10681 atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt aaaagccctc
    10741 tcgggcgcag cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg cgaaacggcg
    10801 cgggtaaatc caccatgatg aaagtgctta ctggtatcta tgctcgcgat gccggcacgc
    10861 ttttatggct ggggaaagaa acgacattta ccgggccgaa atcttcccag gaagccggga
    10921 ttgggattat ccatcaggaa ctgaacctga tcccgcagtt gaccattgcc gaaaacattt
    10981 tcctcggtcg tgagtttgtt aatcgctttg gcaaaattga ctggaaaacc atgtatgccg
    11041 aagcggataa attgctggct aaacttaacc tgcgctttaa aagcgacaag ctggtgggcg
    11101 atctttccat cggtgaccag caaatggttg aaatcgccaa agtgctgagc tttgagtcga
    11161 aagtcatcat tatggatgaa ccgaccgatg cgctgaccga taccgaaacc gaatccctgt
    11221 tccgcgtcat acgcgagctg aaatcgcaag gccgcggtat tgtctatatc tcccaccgca
    11281 tgaaagaaat cttcgagatt tgcgatgacg ttaccgtttt tcgtgacggg caatttattg
    11341 ctgagcgcga agtggcatca ctgaccgaag attcgctgat tgagatgatg gtgggccgca
    11401 agctagaaga tcaatatcca cacctggaca aagcgccggg agatatccgc ctgaaagtcg
    11461 ataatctctg cggacctggc gttaacgatg tctcttttac tttacgcaaa ggcgaaattc
    11521 ttggcgtctc tggtttgatg ggcgcaggtc gtaccgaact gatgaaagtg ctctacggcg
    11581 cactaccgcg caccagcggt tacgtcaccc tggatgggca tgaagtcgtt acccgttcac
    11641 cgcaggatgg cctggcaagc ggcattgtgt atatctccga agaccgtaaa cgtgacggtt
    11701 tagtgttggg catgtcagta aaagagaaca tgtcgctgac agcgctgcgc tacttcagcc
    11761 gcgctggcgg cagtttgaag catgccgatg aacagcaggc tgtgagtgat ttcattcgtc
    11821 tgtttaatgt gaaaacaccg tcgatggaac aggcaattgg tctgctttcc ggtggcaatc
    11881 agcaaaaagt ggcgattgcc cgcggtctga tgacacgccc caaagtgttg atccttgatg
    11941 aacctacccg tggcgtagat gtcggcgcga aaaaagagat ctatcaactg attaaccagt
    12001 tcaaagccga tggcttgagc atcattctgg tgtcatcgga gatgccagaa gtattaggca
    12061 tgagcgatcg catcatcgtc atgcatgaag ggcatctcag cggggaattt actcgtgagc
    12121 aggccaccca ggaagtgtta atggctgccg ctgtgggcaa gcttaatcgc gtgaatcagg
    12181 agtaaaaaaa tgacaaccca gactgtctct ggtcgccgtt atttcacgaa agcgtggctg
    12241 atggagcaga aatcgcttat cgctctgctg gtgctgatcg cgattgtctc gacgttaagc
    12301 ccgaactttt tcaccatcaa taacttattc aatattctcc agcaaacctc ggtgaacgcc
    12361 attatggcgg tcgggatgac gctggtgatc ctgacgtcgg gcatcgactt gtctgtaggt
    12421 tctctgttgg cgctaaccgg cgcagttgct gcatctatcg tcggcattga agtcaatgcg
    12481 ctggtggctg tcgctgccgc tctcgcgtta ggtgccgcaa ttggtgcggt aaccggggtg
    12541 attgtagcga aaggtcgcgt tcaggcgttt atcgctacgc tggttatgat gcttttactg
    12601 cgcggcgtga ccatggttta taccaacggt agcccagtga ataccggctt tactgagaac
    12661 gccgatctgt ttggctggtt ttgtattggt cgtccgctgg gcgtaccgac gccagtctgg
    12721 atcatgggga ttgtcttcct cgcggcctgg tacatgctgc atcacacgcg tctggggcgt
    12781 tacatctacg cgctgggcgg caacgaagcg gcaacgcgtc tttctggtat caacgtcaat
    12841 aaaatcaaaa tcatcgtcta ttctctttgt ggtctgctgg catcgctggc cgggatcatt
    12901 gaagtggcgc gtctctcctc cgcacaaccc acggcgggga ctggctatga gctggatgct
    12961 attgctgcgg tggttctggg cggtacgagt ctggcgggcg gaaaaggtcg cattgttggg
    13021 acgttgatcg gcgcattaat tcttggcttc cttaataatg gattgaattt gttaggtgtt
    13081 tcctcctatt accagatgat cgtcaaagcg gtggtgattt tgctggcggt gctggtagac
    13141 aacaaaaagc agtaataacg tctacaggac atcttgaata tgaacatgaa aaaactggct
    13201 accctggttt ccgctgttgc gctaagcgcc accgtcagtg cgaatgcgat ggcaaaagac
    13261 accatcgcgc tggtggtctc cacgcttaac aacccgttct ttgtatcgct gaaagatggc
    13321 gcgcagaaag aggcggataa gcttggctat aacctggtgg tgctggactc ccagaacaac
    13381 ccggcgaaag agctggcgaa cgtgcaggac ttaaccgttc gcggcaccaa aattctgctg
    13441 attaacccga ccgactccga cgcagtaggt aatgctgtga agatggctaa ccaggcgaac
    13501 atcccggtta tcactcttga ccgccaggca acgaaaggtg aagtggtgag ccacattgct
    13561 tctgataacg tactgggtgg caaaatcgcc ggtgattaca tcgcgaagaa agcgggtgaa
    13621 ggtgccaaag ttatcgagct gcaaggcatt gctggtacat ccgcagcccg tgaacgtggc
    13681 gaaggcttcc agcaggccgt tgctgctcac aagtttaatg ttcttgccag ccagccagca
    13741 gattttgatc gcactaaagg tttgaacgta atgcagaacc tgttgaccgc tcatccggat
    13801 gttcaggctg tattcgcgca gaatgatgaa atggcgctgg gcgcgctgcg cgcactgcaa
    13861 actgccggta aatcggatgt gatggtcgtc ggatttgacg gtacaccgga tggcgaaaaa
    13921 gcggtgaatg atggcaaact agcagcgact atcgctcagc tacccgatca gattggcgcg
    13981 aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga aagttcaggc taagtatccg
    14041 gttgatctga aactggttgt taagcagtag ttttaatcag gttgtatgac ctgatggtga
    14101 cataaatacg tcatcgacag atgaacgtgt aatataaaga aaagcagggc acgcgccacc
    14161 ctaacacggt ggcgcatttt atggacatcc cgaatatgca aaacgcaggc agcctcgttg
    14221 ttcttggcag cattaatgct gaccacattc ttaatcttca atcttttcct actccaggcg
    14281 aaaccgtaac cggtaaccac tatcaggttg catttggcgg caaaggcgcg aatcaggctg
    14341 tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc ctgtacgggt gatgacagca
    14401 ttggtgagag cgttcgccag cagctcgcca ctgataacat tgatattact ccggtcagcg
    14461 tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt taatggcgaa ggtgagaatg
    14521 tcatcggtat tcatgccggc gctaatgctg ccctttcccc ggcgctggtg gaagcgcaac
    14581 gtgagcgtat tgccaacgcg tcagcattat taatgcagct ggaatcacca ctcgaaagtg
    14641 tgatggcagc ggcgaaaatc gcccatcaaa ataagactat cgttgcgctt aacccggctc
    14701 cggctcgcga acttcctgac gaactgctgg cgctggtgga cattattacg ccaaacgaaa
    14761 cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga tgaagatgca gcgaaggcgg
    14821 cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat tactttagga agtcgtggtg
    14881 tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg attccgggtg caggctgtcg
    14941 ataccattgc tgccggagat acctttaacg gcgcgttaat cacggcgttg ctggaagaaa
    15001 aaccattgcc agaggcgatt cggtttgccc atgctgccgc tgcgattgcc gtaacacgta
    15061 aaggcgcaca accttccgta ccgtggcgtg aagagatcga cgcattttta gacaggcaga
    15121 ggtgacgctt ggctacaatg aaagatgttg cccgcctggc gggcgtttct acctcaacag
    15181 tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc gattaccgcc aaagttgaag
    15241 cggcgattaa agaactcaat tacgcgccat cagccctggc gcgtagcctc aaactcaatc
    15301 aaacacatac cattggcatg ttgatcactg ccagtaccaa tcctttctat tcagaactgg
    15361 tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag tctcgtcctt tgcaataccg
    15421 aaggagatga acagcggatg aatcgcaatc tggaaacgct gatgcaaaaa cgcgttgatg
    15481 gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg tgaaatcatg caacgttatc
    15541 cgacagtgcc tactgtgatg atggactggg ctccgttcga tggcgacagc gatcttattc
    15601 aggataactc gttgctgggc ggagacttag caacgcaata tctgatcgat aaaggtcata
    15661 cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc ggcgcgcctg cggttggaag
    15721 gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc tgatggctat gaagtcactg
    15781 gtgattttga atttaacggc gggtttgacg ccatgcgcca actgttatca catccgctgc
    15841 gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt tggcgtttac caggcgttat
    15901 atcaggcaga gttacaggtt ccgcaggata tcgcggtgat tggctatgac gatatcgaac
    15961 tggcaagctt tatgacgcca ccattaacca ctatccacca accgaaagat gaactggggg
    16021 agctggcgat tgatgtactc atccatcgga taacccagcc gacccttcag caacaacgat
    16081 tacaacttac tccgattctg atggaacgcg gttcggctta gatttacgct gtcttttgat
    16141 caaattatta ccatcggttg ttttcagaag catgaacatt gctgctgaag caacagtaat
    16201 aatgcccatc gtgataaacg tatagtggaa ttgttcgaca gtcgttgtgc cttccatccc
    16261 ttcataaacg cgaaggacgg ccgcacttac agcaacgcct aaactaatcg acagttgctg
    16321 cgtgaccgcc agaacactgt taccgctgct ggcgttgtca tcggtcagat cggcaagtgt
    16381 gatggtattc atcgcggtaa attgcgtcga catagccatc cctaatataa acaacggcaa
    16441 gatcagcatc catatcgcca ttgccggtga ttgcaaagag aactgagcga tcattagccc
    16501 aataatcacc gtgatcccca ctaacgtatg gcgatagccc agacgacgta agacttgggt
    16561 aaccatcgat tttgcaataa tggaacctaa cgctgtcggt gccatcatac agccggcaat
    16621 aaacgcctga taaccaaatc ctacctgtaa catcaatggc ataaggaacg gtacacaacc
    16681 ggtccccaga cgggttgcaa tattgcctac gataccgatc gagaaagtgc gggttttaaa
    16741 taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca tggagaatat agagaagcag
    16801 taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag ctggcgacaa tcttttcccc
    16861 gaatagctct attccgcttg agaagagaac aaggctgagg ccaaacagca aaaagccagt
    16921 gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata tgtttgcgcg cgtaaagaag
    16981 gcccgcaata cctatgggga tattgattaa aaatatccag tgccaggttg cccaggtgac
    17041 cagcacgccg ccaagaacgg ggcctaaaat tggccccacc agacccggca tggcgacaaa
    17101 attcaatact ggaagaagtt cattacgagg ataagcgcgc agtaaggcca gccgagcaac
    17161 aggcatcatc attgcgccgc ctatcccctg aataacccgg aagacaacca gctgtggtag
    17221 cgaattagaa agtgcgcagg ccagagaacc caatgtgaac agactcacgg caagggtaaa
    17281 aatgcgacgc gtaccgaagc gatcggctag ccatccgctt accggaataa gcatcgccac
    17341 cgtcagcgta taactgatga tggctgattg catcgcgaga ggagaacgat taaggctatg
    17401 agcgattgcg ggtaaggcgg tattcagaat agtggcatca agtgcctgca tgaagaaggc
    17461 catcgccgcg atccacggca aacccgccat actgcgattc tttttatcgc tcattcaatg
    17521 tcctgttatc gggttgtcac ttatcaggtg agcgtagcag cgcctgacaa gctttaaatg
    17581 ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc ctggtgcaga tccagcttta
    17641 tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata aaccgaatgg aatagcgagg
    17701 cgaatgaggt caaaaacgga ttggcgctca tttcatagat atgctcatgc caggccatat
    17761 cgacttcgat ccagcgttca cggcgaaagt tctcttttaa tgccgccatt tcggccatta
    17821 acgtattgag atgcgccttc tgttccgcgg tgccaaccgt tgcagccagt aggcaagctt
    17881 gcggctccag acagatacgc ataaccagaa agtgatcgat gacctgatga aagttctctt
    17941 ctgtcatcca ccaggtaagc aattcctgat caagaaaatt ccagtttgat tgtggcatga
    18001 cccgggtacc aattcgcggt cgcggtaaaa ccatcccttt tgccgttaac gttttgaccg
    18061 cttcgcgtac cgctgtacga ctcactccaa attgctcgcc cagctcaatc tcaccaggca
    18121 aaatggtgcc gggttcatat tcacctttta agatgcgttg cgccagcttc tcagccagaa
    18181 catacgaaag gtttttctgt gcagctaact gttgtgcgct taaaggcatt actaatcttc
    18241 ctttttcttt ttattcctcc ttagtatgcc accaggaagt gtgattacgg ttgcaaaaac
    18301 ggcaaattgc ttgttttatg gcacattaac gggggttttg ctgaaaaaat gcgcggtcag
    18361 aaaattattt taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact
    18421 gacacggaac aacggcaaac acgttgccgg gtcagcgggg ttctcctgag aatctcaaca
    18481 gagaaaagca aaaaatgctt gactctgtag cgggaaagcg tattatgcac accccgcgc
//
LOCUS       DAZGXZ010000099        18520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000099 DAZGXZ010000000
VERSION     DAZGXZ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.099"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..70
                     /locus_tag="ACHKJF_003609"
     CDS             <1..70
                     /locus_tag="ACHKJF_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0950901.1"
                     /translation="LTQLVMAGLADYQFGCYRRLQS"
     gene            221..1279
                     /locus_tag="ACHKJF_003610"
     CDS             221..1279
                     /locus_tag="ACHKJF_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0950902.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLIAYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRHYTELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            1320..1395
                     /locus_tag="ACHKJF_003611"
     tRNA            1320..1395
                     /locus_tag="ACHKJF_003611"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1353..1355,aa:Ile,seq:cat)
     gene            1403..1479
                     /locus_tag="ACHKJF_003612"
     tRNA            1403..1479
                     /locus_tag="ACHKJF_003612"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1437..1439,aa:Arg,seq:tcg)
     gene            1493..1569
                     /locus_tag="ACHKJF_003613"
     tRNA            1493..1569
                     /locus_tag="ACHKJF_003613"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1527..1529,aa:Arg,seq:tct)
     gene            1655..2590
                     /locus_tag="ACHKJF_003614"
     CDS             1655..2590
                     /locus_tag="ACHKJF_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP0950903.1"
                     /translation="MVFQESNNRDVARKYAEYITSVPLRRFVAEKVREYCGDLPLSIF
                     DGACGSGQLEQFLTVGSLYAVDIQASPLVHIPDNFPDATVKTVNENFFNAIDDVSDTD
                     TFDCVIMNPPFSLQYKAQSADIREKISAMMPYKKTSGVLDDAFYVLASMKARFSFFLC
                     FPGVGYRRGELAMRQYFGNRVVELVQISGGFADTSVSVLFVIIDNEKQSDDVVVYGVG
                     FNGETCKKSAPVTEKINTDNWNRPYIAEEKEVIDIVQVTAELLVRQTRRRKLEDEHNA
                     FVWSLMSDDQRTEVSRFLSVHGLFFDAGSGCVHIR"
     gene            3614..5572
                     /locus_tag="ACHKJF_003615"
     CDS             3614..5572
                     /locus_tag="ACHKJF_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0950904.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISATSDPEYYYVITLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGTPCKYNDIIPADHCLHDVEDMTRLKPPKAKDAEYGCVG
                     QGLHIGKKLLPFIPANAGILLVPCGRGGSGFSTGTEGQYYEASGAIDASSKWGLGTPL
                     YKDLVARTKAALDKNKKNVFLGVVWMQGEADMAAGKHAEHNALFLKMVEGFRTELEKY
                     DAQCVYGSAAKVPWICGDTTYYWKTKYASQYEVVYGGYKGKTDKAIYFVPFMTDENNA
                     NVTTNKPEEDPDIEAIGYYGSKWRDSSTTWTSQYRDSHFSSWARRGIVSDRLATAIVT
                     YAGKSSAFVNGITEVTAPEEAPTSSSSPASEAHNEENTVATSESVILYDAKKASDNQL
                     KPYGWDGMDGRRTLVDDSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVR
                     FKIPENVNLETTRNKYAFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTGNQ
                     QKELGTFGAFDHDWHTLAFKFKGSNSINVTPVFDDVDGQAFDLVKWANTANELNRFVI
                     TDITGTAETYPVLIDTVEVKANKAGAAA"
     gene            5702..5884
                     /locus_tag="ACHKJF_003616"
     CDS             5702..5884
                     /locus_tag="ACHKJF_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0950905.1"
                     /translation="MTFLNQLMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPITP"
     gene            5910..6155
                     /locus_tag="ACHKJF_003617"
     CDS             5910..6155
                     /locus_tag="ACHKJF_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0950906.1"
                     /translation="MSEVKPLVTAEAVKEVLRSEEVLSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGDGSASDGGEPTPDSDMMM"
     gene            6232..6447
                     /locus_tag="ACHKJF_003618"
     CDS             6232..6447
                     /locus_tag="ACHKJF_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0950907.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            6451..7092
                     /locus_tag="ACHKJF_003619"
     CDS             6451..7092
                     /locus_tag="ACHKJF_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950908.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSVLMPSEDANKRCDEQFAAF
                     YDDCIKIARRTFAKILQEKFLPAALSEKYSITVKSAGEEGNKRYFIASAPDKDQEWEC
                     NRPSFVVSGDDGKITISENGKVTPPSHQHSETLIEFAIDYLKNNKKQGLMKRIGRCMG
                     YLQLAAEIEALASGADRDAAVREGLLCDFNTPPFKKEPDGWIQPGLTYLKWRI"
     gene            7120..7347
                     /locus_tag="ACHKJF_003620"
     CDS             7120..7347
                     /locus_tag="ACHKJF_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016050279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP0950909.1"
                     /translation="MKKYDMTVKSFHMGDKNVVVVLGIYNYQVPSMLLFNIEIDIERR
                     EDATIPFYEALALKKAASLIHEISDDLAVAA"
     gene            complement(7352..7639)
                     /locus_tag="ACHKJF_003621"
     CDS             complement(7352..7639)
                     /locus_tag="ACHKJF_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015976796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950910.1"
                     /translation="MEISNHELFQLIQGLQRENIAINTAANFLFYNIVDILDQQSGDK
                     KFSDELKTRLHSELSKITTSGKPEIKVAINTLMQPPVRPMFGNTSPEPFLK"
     gene            7764..8297
                     /locus_tag="ACHKJF_003622"
     CDS             7764..8297
                     /locus_tag="ACHKJF_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0950911.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMTLSKGKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            8454..8638
                     /locus_tag="ACHKJF_003623"
                     /pseudo
     CDS             8454..8638
                     /locus_tag="ACHKJF_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8653..8784
                     /locus_tag="ACHKJF_003624"
     CDS             8653..8784
                     /locus_tag="ACHKJF_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950912.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVAICFSTAWRVLTLSGLVG"
     gene            8831..9727
                     /locus_tag="ACHKJF_003625"
     CDS             8831..9727
                     /locus_tag="ACHKJF_003625"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0950913.1"
                     /translation="MPSLLPGSVDLVLCDPPYGTIKGLTLRTWDKSTTAWDDALNPED
                     IFTQCERLLRVGGVLVMFSQEPHTSTMITLADKNLPFLYRMVWKKEHFPNPLSVKKAP
                     VSLFEDIVVFRKKYDSEKNHPLREYARNILSFIGHRTDKEVNKTLGHRRAEHFFCVNT
                     LQFSLCSQDTYNELIKRFGIDRMPGFMTYEEMRKVNSGYGTVVFNLPQGKKYRANILE
                     YARERERWHPTQKPVALLEELIQTYSNPGDRVLDFTMGSGSTGVACIRTGRRFTGIEK
                     DDRYFAVAKKRLEKENSEMVVI"
     gene            9944..10129
                     /locus_tag="ACHKJF_003626"
     CDS             9944..10129
                     /locus_tag="ACHKJF_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0950914.1"
                     /translation="MRELKMKLCVLMLPLVVSACGLKPPVQAPCVKPPPPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            10215..10439
                     /locus_tag="ACHKJF_003627"
     CDS             10215..10439
                     /locus_tag="ACHKJF_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950915.1"
                     /translation="MKKLLVTVKSFQGTIPFRILQCGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            10784..11110
                     /locus_tag="ACHKJF_003628"
     CDS             10784..11110
                     /locus_tag="ACHKJF_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP0950916.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(11242..11442)
                     /locus_tag="ACHKJF_003629"
     CDS             complement(11242..11442)
                     /locus_tag="ACHKJF_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0950917.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            11484..11849
                     /locus_tag="ACHKJF_003630"
     CDS             11484..11849
                     /locus_tag="ACHKJF_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0950918.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVREAASGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            12139..12702
                     /locus_tag="ACHKJF_003631"
     CDS             12139..12702
                     /locus_tag="ACHKJF_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0950919.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            12699..14360
                     /locus_tag="ACHKJF_003632"
     CDS             12699..14360
                     /locus_tag="ACHKJF_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0950920.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQNGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVERGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWTGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLEHIGIDPSGVGQIL
                     DSLVEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            14424..16361
                     /locus_tag="ACHKJF_003633"
     CDS             14424..16361
                     /locus_tag="ACHKJF_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0950921.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFS
                     AASGNRKPMVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGSASGG"
     gene            16406..16627
                     /locus_tag="ACHKJF_003634"
     CDS             16406..16627
                     /locus_tag="ACHKJF_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950922.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRQGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            16573..18520
                     /locus_tag="ACHKJF_003635"
                     /pseudo
     CDS             16573..18520
                     /locus_tag="ACHKJF_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT         4534 a         4251 c         5380 g         4355 t
ORIGIN      
        1 actgacccag cttgttatgg cgggtctggc agactatcag ttcggttgtt acagacgcct
       61 tcagtcctga aggctttttt atttgtggta aatgggcggc tggtgggtgt taggggcact
      121 caccagccat ctgctcatgc gtccggatta caagcaaacc tcaggcccac tgctttgcgc
      181 aaaagcagaa tgagcctatc agagacaggc ttaatgatcc atgcttaata ctgtaaaaat
      241 atccagttgt gagttaatca acgccgactg cctggaattt atccggtcgt tacccgaaaa
      301 ttctgttgac ctgatagtca cggacccgcc gtactttaaa gtgaagcctg agggctggga
      361 taaccagtgg aagggcgacg atgattacct gaagtggctg gaccagtgtc tggcgcagtt
      421 ctggcgggtg ctgaaacctg ccggaagtct ttacctgttc tgtggccatc gcctggcatc
      481 tgacattgaa atcatgatgc gtgaacgctt cagtgtgctg aaccatatta tctgggcgaa
      541 gccgtccgga cgctggaacg ggtgcaacaa ggaaagcctg agggcgtatt tccccgccac
      601 agagcgcatt ctgttcgcgg aacattatca ggggccgtat cgtccgaaag atgccgggta
      661 tgaggcgaag ggcagggcac tgaaacagca tgtgatggcc ccgctgattg cttactttcg
      721 tgatgcacgt gctgccctgg ggataacggc aaaacagatt gctgatgcca caggaaagaa
      781 aaacatggtg tcgcactggt tcagtgccag tcagtggcag ctaccggacg aaagcgatta
      841 tctgaaatta caggcgctgt ttgcccgggt ggcagaagag aagcatcagc gcggagaact
      901 ggaaaagcca catcaccaac tggtcagcac atacagtgaa ctgaaccgac actatacgga
      961 actgcagagt gaatataagc atctgcggcg gtatttcggt gtgacggcgc aggtgccgta
     1021 caccgatgtg tggacgcata aaccggtgca gtactatccc gggaaacatc cgtgcgaaaa
     1081 accggcagaa atgctgcagc agataatcag cgcgagcagt cgtccgggtg acctggttgc
     1141 agattttttc atggggtcgg gttcgacagt caaagcagcg atggcgctgg ggcgtcgtgc
     1201 aactggcgtt gagctggaga ctgaacgttt tgagcagacg gtcagggaag ttcaggattt
     1261 agccagtcag aacggatgat attgcaggat tagttacgta ccgttattat cctgcgcccg
     1321 gccctttagc tcagtggtga gagcgagcga ctcataatcg ccaggtcgct ggttcaaatc
     1381 cagcaagggc caccatcaca taccgccatt agctcatcgg gatagagcgc cagccttcga
     1441 agctggttgc gcggggttcg agtcctcgat ggcggtccat tatctgtaca ctgcgttgtt
     1501 agctcagccg gacagagcaa ttgccttcta agcaatcggt cactggttcg aatccagtac
     1561 aacgcaccac acttattttt cctggctcgc ttttgtgggc cttttttgcc tccgcgccac
     1621 gcccggcgca tatcaaccac agagcctttc acaaatggtt tttcaggagt ccaataatcg
     1681 cgatgtcgcc agaaaatacg cagagtacat cacgtccgtt cctttgcgtc ggtttgtcgc
     1741 tgaaaaagtg cgggaatatt gtggtgatct gcccctgagt atttttgatg gtgcgtgtgg
     1801 ctccgggcaa ctggagcagt ttctcacggt gggaagtctg tatgctgtgg atatacaggc
     1861 ctctccgttg gtgcatatcc cggataattt cccggatgcc acagtgaaga cggtcaatga
     1921 aaatttcttc aacgccatcg atgatgtcag tgacacggac acgttcgatt gtgtcatcat
     1981 gaatccgcct ttttcgctac agtacaaagc acagagcgcg gatatcaggg agaagatttc
     2041 agcgatgatg ccttataaaa agacctccgg tgttctggat gacgcgtttt atgttctggc
     2101 gagcatgaag gcaagatttt catttttttt gtgctttccc ggggttggtt acaggcgtgg
     2161 tgagctggca atgcgtcagt attttggcaa ccgcgttgtt gagttggttc agatttcagg
     2221 cgggttcgct gatacatcgg tttctgtatt attcgtcatt attgataacg aaaagcaaag
     2281 cgatgatgtt gttgtttatg gcgtcgggtt taatggggaa acttgcaaaa agtcagcgcc
     2341 ggtgacggag aagattaaca cagacaactg gaacaggccg tacattgctg aagagaagga
     2401 ggtcattgac attgtacagg tgacggctga gttgcttgta aggcaaacaa gaagacggaa
     2461 acttgaagat gagcataacg cctttgtgtg gtccttaatg agtgatgatc agcggacgga
     2521 ggtttcccgt tttctgtcag tgcacggttt gttttttgat gccggttcag gttgtgtgca
     2581 catacgttaa atctggtgcc ggtcgctggt tgccggtgtc gtgatgattg tcttcctgtt
     2641 gccccgaaaa ttttaaatgt ctcacaattc agacggttga cagttgtctg atttgcgggg
     2701 agtttgttaa aagaaactgg catggtgaat ccccctgagc ggaggggcat atcagcgaca
     2761 ggtgtttggt tacatccctt atcctttctg tgcgggttca ggtgctgata ctgaactcac
     2821 cgggaggcac ccggcaccat gcgcatgatg atacagatac gcagcgtcag cccctctccg
     2881 gaggggcttt tctgtgccgg atacatcaca gtttctggaa ccttgggtac tacagtatca
     2941 gtcagggtgc tatattttca gatgtgatga aagcctgtca gcaggcaggg cgtatcggaa
     3001 atgacccagt agagaaaacg ttgactcaga taccggtgct gagttaccgg gaaaccggca
     3061 tcacatgacc gctatccttc caggcccgtc cgctccggtg ggccttttta ctgcagaaaa
     3121 caggttcccc gttaaatgct atgttgctca caattcagtc agttgaaggt tgtcttcccg
     3181 actgagaatt tgttaaaaaa tttctgcatg atgaatcccc ctgtgcggag gggcggctgg
     3241 tgtacggtat ggtctctgat gatcgttagc gagaatgacg cgggttcggt ggcaccgggc
     3301 tgaactcacc gggaggcacc cggcatcatg caatgaacgg tacatagcgc tactccagcc
     3361 cctctccgga ggggctttct tatggataaa aaaagcccgc atggtgagat gcgggcagca
     3421 aggaataaac aacaaaacgt gaagtaatca atttttcagc tggcgaataa tacccgacag
     3481 taatcactct gcgcaactgc gcggcctttt tcgtattgcg ggctgtagtc ttccttctgt
     3541 cattgtcctg taacttccgg acttcagccc gccccttatc tgactcacaa cattatcccg
     3601 accgggagga ttcatggcat ttaaacacta tgacgtggtc agggcggcgt cgccgtcaga
     3661 ccttgcggag cgactgactc aaaaactgaa ggaggggtgg cagccatttg gcagcccggt
     3721 ggccatcacg ccttataccc tgatgcaggc cattgcggcg gaaggtgatg tcaccacacc
     3781 tgtggtggtg aagccgtcgg atggagaagg cacagttatc agcgccacca gcgacccaga
     3841 gtattactac gtgattacgc tggctggcca gtcaaacagc atggcatatg gtgaaggcct
     3901 tccgctgccg gagacatatg accgtccgga cccgcgtatt aagcagctgg cgcgtcgcag
     3961 tacagtgaca ccgggaggta ctccctgtaa atataacgac atcattccgg cagaccattg
     4021 tcttcatgat gttgaagata tgaccaggct aaaacccccc aaagctaaag atgcagaata
     4081 cggttgtgtt ggccagggtt tgcatattgg taagaaacta ctgccattta tccctgctaa
     4141 tgcaggtatt ctgctggtcc cttgtggacg tggcggatca ggctttagta ccggaacaga
     4201 aggacagtat tatgaagcct ctggcgcaat cgacgcatca tcaaaatggg ggcttgggac
     4261 acctctatat aaagatctgg ttgccagaac caaagctgca ttagacaaaa acaaaaagaa
     4321 cgtatttctt ggcgttgtct ggatgcaggg tgaggcagat atggctgccg gaaaacatgc
     4381 agaacacaat gcacttttcc tgaaaatggt tgaaggtttc cgcactgagt tagagaaata
     4441 tgatgctcaa tgcgtatatg gtagtgctgc gaaagtcccc tggatttgcg gcgataccac
     4501 gtattactgg aaaaccaaat acgcgtctca atatgaagtt gtgtacggtg gttacaaagg
     4561 gaaaacggat aaggctattt attttgttcc gtttatgacg gatgaaaata atgcaaatgt
     4621 tacaaccaat aagcccgagg aagacccgga tattgaagct attggatatt atggctccaa
     4681 atggcgtgat agttcaacaa catggacgtc acagtacaga gatagccatt ttagctcatg
     4741 ggctcgtcga ggtatcgttt cggatcgact ggcaacagca attgtaacct atgcaggtaa
     4801 atcatctgcc tttgtgaatg gtattacaga agtaacagca ccggaagagg cgccaacatc
     4861 ttcttcatcg ccagcttctg aagctcataa tgaagaaaat acggttgcca cttcggagtc
     4921 tgtcattctc tacgatgcca aaaaagcttc ggacaatcag ctgaaacctt atggctggga
     4981 cggtatggat ggcagacgca cactggttga tgacagcggc aataaagctc tgcgaattga
     5041 gaaaaataac agcgcgaaat cctggtcaat gtactgtgat attgctgcag acaaggcaaa
     5101 actgttactg gaaaaaggcg gggaaattgc tgtccggttt aaaatccctg aaaacgtcaa
     5161 tcttgaaaca accagaaaca agtatgcctt tggtttgtac tggcgaatag cggaatggcc
     5221 gggtgagggt ggtgaaggct atctgagttc tttctttgtc cagacagata aagccagtat
     5281 tgatgttgca taccatcata caggtaatca acaaaaagaa cttggcacgt ttggcgcatt
     5341 cgaccatgac tggcatacgc ttgcatttaa atttaagggc agtaacagca ttaatgttac
     5401 tccggtgttt gatgatgtgg atggacaggc gtttgacctg gtgaaatggg ccaatactgc
     5461 taatgaactc aacaggtttg tcattacgga tattacaggt actgctgaaa cttaccctgt
     5521 acttattgat acggtggaag ttaaagcaaa caaagctgga gcagccgcat aattgctaaa
     5581 aaaagccgcc agcggcagga atggaagctg gcggctggta aaacgaccat gatgacaccg
     5641 aagtctgcgg tgtttttcta caaaaataag taaatcatgt caactatcac ggtgagaaac
     5701 catgacattt ctgaaccagt taatgctgta cttctgtacg gcggtctgtg tgctgtatct
     5761 tctttcgggt gggtacaggg cagtgcgcga tttctggcgc aggcagattg ataaaagggc
     5821 cgctgagaaa atcagcgcca gtcagtcagc cggaagcaaa cccgaagagc ccattactcc
     5881 ttaataaccc ctttcagcga gaaaatccta tgtcagaagt aaaacccctg gtcactgctg
     5941 aagcagtgaa ggaagtcctg cgctctgaag aagtcctgag cgcactgaaa cagaaactcc
     6001 gccagaacct tgagtcgcgt cttgatgcag aagtggatgc cattctggat gaactgctgg
     6061 gcgcaccggc tgttccggag ccggaaggca ttgcgggtga cgggagtgct tcagatggcg
     6121 gtgaacccac acccgacagc gacatgatga tgtaagcatg cgcaagggac catcggtgtg
     6181 tgccggtggt ctttatattg ttgtgagctt ccggattgcg ggagacgggg tatgtaccag
     6241 atggaaaaaa tcacaacagg tgtgtcatac accacgtcag cggtgggaac gggctactgg
     6301 ttcctgcagt tgctggacag ggtttccccg tctcagtggg cggcaatagg cgtgctgggg
     6361 agtctgctgt ttggtctgct gacgtacctg acgaacctgt atttcaagat taaagaagac
     6421 cgtcgtaagg cggcgcgggg agagtagacg atgaaccatg aagaaatgaa tcagcgcttc
     6481 agtcgtctgg aaaatgaaat tgctgaactg aataaaaaac tgtcggtgct gatgccttct
     6541 gaagatgcaa acaaacgctg cgatgagcag tttgcggcgt tttacgatga ttgcatcaaa
     6601 attgctcgca ggacctttgc gaagattttg caggaaaagt ttttaccggc cgcattgtca
     6661 gaaaagtact ccattacggt taaaagtgcc ggagaggaag gcaataagcg ttattttatt
     6721 gcgtctgcac cggataaaga ccaggaatgg gagtgtaacc ggccatcctt tgttgtatcc
     6781 ggtgatgacg ggaaaatcac catatcagaa aatggaaaag taacaccgcc atcgcaccag
     6841 cacagtgaga cgctcattga atttgccatt gattacctga agaacaataa aaaacagggg
     6901 ctgatgaagc gcattggtcg ctgcatggga tatctgcagc tggcggcaga gattgaagca
     6961 cttgccagtg gtgcagacag ggatgctgcg gtgcgggagg gtcttctttg tgatttcaat
     7021 acgccaccct ttaagaaaga gcctgatggc tggatccagc cggggctgac ttatctcaaa
     7081 tggcgcatat agtaaaagag aaaacaattg aggtgaaaaa tgaaaaaata cgatatgaca
     7141 gttaaatcat tccacatggg ggataaaaat gttgtcgttg tccttggtat atataattat
     7201 caggttccat caatgctgtt gttcaatatt gaaatagata ttgaacgacg ggaagatgct
     7261 accattccgt tttatgaggc gctggcttta aaaaaagcag cctcgctaat tcatgaaatt
     7321 tctgatgatt tagcagtggc tgcttagatt tttatttcaa aaatggctca ggactggtat
     7381 tgccaaacat gggtcttacc ggtggttgca tgagggtatt gatggcgact ttaatctctg
     7441 gtttacctga ggttgtgatt ttgctcagtt cgctatgtaa cctggtttta agctcatcac
     7501 tgaatttctt gtctccactt tgctgatcca gaatgtcaac gatgttgtag aacaggaaat
     7561 tggctgcggt atttatagct atgttttcgc gctgaagccc ctgaattagt tgaaaaagtt
     7621 catggtttga tatttccatt aagttctcct tattgaggtt atcagccatc cctcttttgt
     7681 tacatgtgcc agtgcccacc actggcaggc tgaagactta acatatccag ggattcggaa
     7741 ccgataaatc ctgataaata tccatgaaca ccaaaatcaa atacggcctg tcggctgccg
     7801 ttctggcgct gattgccgca ggtgcgcctg cgcctgaaat cctcgaccag tttcttgacg
     7861 aaaaagaagg taaccacacc acggcatacc gtgatggtgc gggtatctgg accatctgcc
     7921 gaggtgccac ccgggtggat ggtaagcctg tgattcctgg catgacgctg tcgaagggga
     7981 aatgcgacca ggttaacgcc attgagcgtg ataaagcgct ggcatgggtg gagaaaaaca
     8041 tcagagtgcc actgaccgaa ccccagaaag cggggatcgc gtcattctgt ccttacaaca
     8101 ttggccccgg taagtgtttc ccgtcgacgt tttataaacg aattaatgca ggcgatcgca
     8161 ggggagcgtg tgaagcgatt cgctggtgga ttaaggacgg tggcagagac tgccgtatcc
     8221 gctcaaataa ttgttatggt caggtatccc gtcgtgacca ggagagcgcg ctggcgtgct
     8281 ggggtatcga cagataagca gaatattttg ctgaaaaatg acgttgacta acgcggacgg
     8341 ataacacgaa atcctgcgaa ctggcaaaac ctaagtgaat aaaagtaaaa accccgtttg
     8401 ttggcagcaa gcggggtttt gtgtttctga ccttggataa ggcaagggag aacatggaaa
     8461 agtataaacg aattctgttg aggttgacta tgaaaaacgg ccttgaactg aaagcgccag
     8521 agtaactgat gacatcagca gagcgctggc ttttgctatt aagtgggtgg cggttggtat
     8581 cgctgtgtct ccgatactgt atgggctggc aaaactggtc attgcgttga aatcgtgaag
     8641 ggaggattaa gcatgtcaga caaactcata atgctggcga agatcctctg tgtaatcgtc
     8701 ggcatttcat tttcactaat gctggttgct atttgctttt ccactgcctg gcgcgtctta
     8761 actctgtcag ggctggtggg gtgacagaaa catgaaatgt gtactgtatt ccggtgactg
     8821 tcttgatgtt atgccttcat tactaccggg aagtgttgac ctggttttat gtgacccgcc
     8881 gtatggaacc attaaagggc ttacactccg tacatgggat aagagtacga cagcatggga
     8941 cgatgcctta aatcctgaag atattttcac acagtgcgaa cgactgctga gggtgggcgg
     9001 tgtgctggtc atgttcagcc aggagccgca tacaagcacg atgattactc tggcggataa
     9061 aaacctgccg tttttatatc gtatggtgtg gaagaaggag catttcccca acccgctttc
     9121 agtaaaaaaa gcacctgtat ctttattcga ggatatcgtt gttttccgga aaaaatatga
     9181 cagtgagaaa aaccacccgc tgagggagta cgcccgaaat atcctttctt tcattggcca
     9241 caggacagac aaggaagtta acaaaacact ggggcaccgt cgggcagagc attttttttg
     9301 cgtgaacaca ctccagtttt ccctctgctc acaggatacg tataacgagt taattaaacg
     9361 ctttggtatc gaccgtatgc cgggtttcat gacatatgaa gaaatgcgca aagtgaattc
     9421 cggttacggc actgtggttt ttaatctccc tcaggggaaa aaataccgcg caaatattct
     9481 ggagtacgcg cgggagcggg agcgatggca tccgacccag aagccagttg cattactgga
     9541 agagctgatt cagacgtaca gcaaccctgg cgacagggta ctggatttta ctatggggag
     9601 tggttcaaca ggtgttgcat gtatccggac ggggagacgg tttaccggaa tagaaaagga
     9661 tgaccggtat ttcgctgttg caaaaaaacg tctggaaaaa gaaaacagtg aaatggtggt
     9721 gatatgaacc gtgttctgtg tgtggtgatt attgtcctgc tggtggcctg tggtgcgctt
     9781 agtctggggc tgaatcatta ccgcgataac gccatcacct acaaagcgca gcgcgataaa
     9841 aaagccagag agctggagct ggcaaacgca accattactg atatgcaggt gcgccagcgc
     9901 gatgttgctg cgctcgatgc aaaatactcg agggaattag ccgatgcgag agctgaaaat
     9961 gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc tgcgggttaa agccacctgt
    10021 ccaggctccg tgcgtgaagc ccccaccacc tccggcgtgg ataatgcaac cggcccccga
    10081 ctggcagaca ccgctgaacg ggattatttc atcctcagag aacggttgat gacaatgcag
    10141 aagcagctgg aaggggcgca ggaatatatc cgcactcagt gtattaacta gtatttttgt
    10201 tatccggaga atgcatgaag aaattactgg taaccgtaaa gtcttttcag ggaacaattc
    10261 cgttccgtat tttgcagtgt ggtcgtgttc ttgttgaagg ttcgttcagt ggtaaatgta
    10321 cgcaattaca ctcccggacc tttcaggtga atgccacgaa tgaagagcta accgttgagt
    10381 gtacgatgaa tgccgctaaa tgccgcatgg tatccgctgc attacagcca gtgtgttgag
    10441 cgaccttatt atccatgcgc ggtattgtcg ccgtattcct gcattaacag agaccgcagc
    10501 ccgaccggga gactcctctg cgcgagtgtg cggggataat caaaaacgat acacaccggg
    10561 gtttaccgcg ttaacggagc gcggcgttgt cccctcatgg tcgctggtcc ggtgcgatgg
    10621 tggaagaaac cggacgatgt gttacctcgc aagctctgtt atgtcatgtg tctgatttgt
    10681 gatttaagtc ggataattgt cgttgccatt aagcagagga ttgatgaccg acagggtggc
    10741 attgttagaa taagacttat tcttatctgt gccgggaatg aaaatgaaaa gaaatcttcc
    10801 gttaattatt ttgttgtctt ctctggttat gggctgtacg caacataaaa cagatatgcc
    10861 ccgacagttg gttaaggcat taccacaata tccggcctat gcagcggcaa attatataaa
    10921 gggacgggtt gatgtgaagt ttgatattgg tgctgatggt actgtcaccc gaattgagtt
    10981 tatccgttca gagccgcacc atctgtttga tgagcaggtt gtaaaagcga tggcaaaatg
    11041 gcgatttgag aaggacaggc cgcgtaaagg cgtgaagaaa acgtttatct ttagtccttc
    11101 tgcaccctga ttatttcatc agaaattaat tatcactctg ttgttattct gtacatcccg
    11161 gcagggtaag tcttgttccg tcggatatga agatgaaata ttgttggagg acagtgggta
    11221 cctgctcctg taaccgaacg ttcatttctc gttatttgtc atgctggccg ggcgcagatg
    11281 cgttgcatct gttgccagcc ttctcctgca ggcttcaata acccacgctg aaaagttacc
    11341 ggacccttta tgctcaaggg cgatgttgat ctgttcaatc atgtgattgg ggaaacggat
    11401 attgcgggtt gtggttctgc gggtccggtt tttcgatgac atatttattt cctttactga
    11461 ttgccatatg acggggattt tacatggctc agcttcgtac actccagagc agaatcaaaa
    11521 cactgaatac ccgacgggtg aatattctga agggtgaaca gcgtcgtgtc agtggcagtg
    11581 cacgtgtttc cctcaagcgt catatctggc tcagggacgc cgggcagtgc tgtctctgtg
    11641 gtcgtgtggt tgacctctgt gacagtgaac tcgatcaccg aattgcactt cagttcggtg
    11701 gtggtaatga ggagacgaat ctctggacgc tctgtaccga atgccatcga caaaagtctg
    11761 ttcgtgaagc ggcgagtggt atgccggacc cgacactgcc ggaggtgtcc ggaggtagtg
    11821 gcagagcgga cgacatcatc ggactgtaac ccgacccggg ggggtatcat ccggcgtaaa
    11881 aaactatcgc tccggacacc gcgccccctc tcacgcagag aaaaaattcc cgtttcaggg
    11941 cagttaacat gttaactggc tgcccgggca tttttgcggt ttttatcttt attattcagt
    12001 ttgttgcgtg gaaaaaatgt taacaggctt tttcagcaaa tgttaaccag gcagcagtta
    12061 acatttgcgg catgagacgc cgggaaaaat gggctgaacc atacccggct gagtgcgttc
    12121 tggacccggg aggaggctgt gctgacaacg caaaaacgaa aatttgcgct ggcgctcatg
    12181 tccgggaaaa acaaaacagc gtcagccatt gccgccggtt attcggcgaa gacagccagg
    12241 gttaaaggct cgcagctggc aaaagatccg gaggtgcttg cgtttatagc ccgtaaacag
    12301 tgcgagacgg tggaggtgga tgaggttcct gtttaccggc agaaaaaatc agagcaggag
    12361 gataaacccc gtcgccgtga ggcggctgca ataccacagc cggacgaaac aaatccggag
    12421 atgccaccgc ccgtggtgat atctcctggt attgagtata tggaggacgg tcttcccgat
    12481 ccggtgaaag cgatggggcg tcttctggtg gagaacatta ataccgaccc caggctggcg
    12541 ctggatgcgg cttataagct ggcgcagttc acgcaccaca aaaaagggga tgccggtaaa
    12601 aaatcggcaa aaggtgacgc ggcgaaaaaa gcggctaacc gttttgcggt gccaccacca
    12661 ccccgactgg tggtgaataa tgataatgag ggcaacggat gatacctgtg tggagcacgg
    12721 cctgcccgga ctgggcagag cgcctgaaaa aggggctgtc gattattccg gctccgattt
    12781 atccggagca ggccgcacat gccctggcga tttttaaaca actgcggatt gtggatgcac
    12841 cgggcagccc gacgttcggg gagtcctgtg caccgtgggt gtttgacctg gtggcggccc
    12901 tgtttggctc ctacgatgcg cagaacggtg tacgccatat caaggaagtt tttatcctta
    12961 tcccgaaaaa aaacagcaag tccacgctgg ctgccgggat catgatgacg gcgctgttac
    13021 tgaactggcg gcaggcggcg ggctacacca ttctggcccc gaccgtggag gtggcggcta
    13081 acgcctttaa tcctgcgcgg gatatggtca gacgtgacga tgatctggat gacctctgcc
    13141 aggtacagac acatatccgg accatcaccc acagggtgac ggacaccacc cttaaggtgg
    13201 tggcagccga tccgaatacg gtgtccggta tcaagtctgt ggggacactg attgatgaac
    13261 tgtggctgtt tggcaagcag tacaaagcgg aggacatgtt acgtgaagcc atcggcgggc
    13321 ttgcctcccg cccggaaggg tttgtggtgt atacgaccac ccagtcgaat gagccgccag
    13381 ccggggtgtt cagacagaaa ctgcagtacg cccgggatgt ccgtgacggc aaaattcatg
    13441 atccgcactt tctgccggtg atatttgagc atccgccgga gatggtggag cgcggtgagc
    13501 acctgctgat ggaaaacctc gccatggtca atccgaatct cggttattcg gtggatgagg
    13561 cttttctgta ccgggagtac cgtaaagccc gggaggctgg tgaggaagca tttcgtggct
    13621 tcatgtcaaa acatgccaat gtggaaattg gtcttgccct gcgttctgac cgctggacgg
    13681 gtgcggattt ctgggagcag cagggcaggc gcgtcagcct ggacgatatc ctgcagcgcg
    13741 ctgatgtggt gacggtgggg attgacggcg ggggcctgga tgatctgctg ggaatgtacg
    13801 tgattggccg tgacagggaa acccgcgaat ggctgggctg gggccatgcc tgggcgcatg
    13861 aaaccgcggt ggtcagacgg aagagtgagg catcccggtt tcaggatttt gtggcctgtg
    13921 gagacatgac gattgtccgt cgggtcgggg atgacacggc ggaagtggcg gagtatgtgc
    13981 gtcgtattca tgaggctgag ttactggagc atatcggtat tgacccgtca ggtgtggggc
    14041 agattctgga ttcactggtg gaagccggga tccccgacgg aattgttgtg gggataagcc
    14101 agggctggaa gctgggcggg gccataaaaa ccaccgagcg caaactggct gagggagtac
    14161 tggtgcatgg tggtcagcca ctgatggcct ggtgcgttgg caatgcccgg gtggagccga
    14221 aaggtaacgc catccttatt accaaacagg ccagcggacg ggggaaaatt gacccgctga
    14281 tggcgctctt caatgcggta tccctgatgt ccctgaatcc ggagccgaaa aagaaagaat
    14341 atgcggtttt tttcatataa ccctgttcac cctgtaacca tcatggaccg ctgcggcggt
    14401 ttttttattt tcaggaggct gatgtgactc ttaaacgggc ctgctccctg ctgacggtga
    14461 aatccttcag tgaggatgag cgggtgatca ccgggattgc gtcaacgcct tctccggatc
    14521 gggatggtga catcctggag ccggagggcg cggagtttgg cagtgcgatc ccgtttctct
    14581 ggcagcatga ccattcccgc ccggtggggc agtgtacggt gcgccgggtc agcgaagggc
    14641 tggaaatcac ggcaacactg gcgaagcccg tgccggatat gccatcgcaa ctggctgcca
    14701 ggctggatga ggcctgggcg gccattaaga ccgggctggt cagggggctg tccgtgggct
    14761 tccgtcctca tgaatacacc tttctggacg gaggcggact gcattttctg cgctgggagc
    14821 tgatggaggt gtctgccgtc accgtgcccg cgaatgcgga atgcaccatc cggaccatta
    14881 aatcttacga ccgccagttt tctgccgcgt ccggcaaccg gaaaccgatg gtgaaaatcg
    14941 catcttctgc cggcgctgcg gcacagtcaa caaccgtttt tcataaggaa aagaccataa
    15001 tgaatattgg cgaacagatt aaaagttttg aaaacaagcg tgcagcactg gcagcctccc
    15061 ttgaggaggt catgaccaaa gccgcagagg aagggcgaac gctggatgtg gaggaggaag
    15121 agcattacga caacaccgca gcggaaatac gtcaggtgga tgcgcacctg aagcgcctgc
    15181 gtgaactgga agccggtaag gccgccacgg cgcagccggt gaaacaggcc ggtaacggga
    15241 atgtggccgc ggtggcttct gcgccggtga tccgtgtgga gcagaaactg gataagggga
    15301 ttggtttcgc acgttttgcc aaatcgctgg ctgcggctaa aggtgtccgc tctgaagccc
    15361 tggaagtggc ccgtcgtcag tatccggatg acagtcgtct gcatcatgtc ctgaaatcgg
    15421 cagtgggcgc ggggaccacc acggatccgc agtgggcagg cagcctgtct gaatatcagg
    15481 aatacgcaca ggactttatt gattacctgc gtcctcagac cattatcggg cgatttggtc
    15541 agggcgggat ccctgcactt cgtcaggtgc cgttcaatat ccgtgtgcac gcccaggtgt
    15601 cgggcggtgc tgccggctgg gtgggggagg gtaaggcaaa acccctgacg aagtttgatt
    15661 ttgaatccat caccttcagt catgcgaagg tgtcggccat tgcggtactg acggaagaat
    15721 tgatccgttt ttccagtccg gctgctgatg cactggtccg taatgcgctg gcggaagcgg
    15781 tggtggcgcg tctggataca gactttgtgg acccgaaaaa agccgcagtg gcagatgtct
    15841 ccccggcgtc catcacccat gatgtgaagg gcacggcatc aaccggtaac ccggatgcgg
    15901 atgcagaggc ggcgtttgga cagtttgtgg cagcaaacct gcagcccacc ggtgctgtct
    15961 ggctgatgtc cagcaccaat gccctggcgc tgtccatgcg taaaaatgcg ctgggtcaga
    16021 aggaataccc ggacatgacc ctgctggggg ggaccttcca ggggctgccg gtgattgtct
    16081 cccagtacgt gggtgaccag ctggtgctgg tgaatgcccc ggatatttat ctggcggatg
    16141 acggcggcgt ggcagtggat atgtcccgcg aggcatcact ggaaatgcag tctgagccga
    16201 ccggcgacag taccacgccg tcgccggtgg agctggtttc catgttccag acaggcagtg
    16261 tggccatccg tgcggagcgc tggatcaact ggcgtcgtcg ccgtaccgcg gcggtggcgg
    16321 tgatcaccgg tgtgaactac ggcagtgcgt ccggcggctg agtctgataa ggaggacggg
    16381 aggcgtgcgc ctcccgtaac aggttatggc aaagatccga tacctgcagg gcacgcatga
    16441 tgcccgggcc ggggatatcc gggatgtggc acagccgtgt gcggaggtgc tggttcgcca
    16501 gggaaaggcg gagtacatca cggtgcgacg tccggcaggt cagaaaaaga aacgtgatgc
    16561 ggagcatggc gaatgtggaa ccttttacgg cgaacccgaa aaaaccagaa atcaggacgt
    16621 gacgtaagag aggcgggctg gaccagcctg tttcaggcgg tggctgagcc cttttccggt
    16681 gcctggcagc agggcgtgaa agccgatcct gaagccgtcc tctcttttca tgcggtgttt
    16741 gcatgtattt cgctgatatc ccaggatatc gccaaaatgc ggctgcgtct tatgcagacg
    16801 gatgcgcagg ggatacgcag ggaaacgcgc cggggggata ttgcccgcct ctgtcgtcgt
    16861 cccaacgccc agcagaaccg catccagttt tttgaactgt ggctgaacgc caaactgcgt
    16921 cacggcaata cggtggtgct gaaaatccgt aatgcccggg ggcagatcaa agaactgcgt
    16981 attctggact ggaaccgggt tgaacctctg gtggcggatg acggcgaggt gttctaccgc
    17041 atcacgccgg accggaactg tgggatcact gaggcggtga cggtgcctgc ccgggaagtg
    17101 atccacgacc ggtttaactg tttttttcat ccgcttatag ggttgccgcc ggtgtatgcc
    17161 gccgggctgg cagccacgca ggggcatcat attcaggaaa attcgacgtc ttttttcaga
    17221 aatggcggca ggccgtccgg ggtgattgag atccccggca gtattacgga agaaaatgcg
    17281 aaaaaactga agagcaactg ggacagcggg tatacaggcg aaaatgcggg gaaaacggcc
    17341 attctgagca acggggcaaa atacaacccc acgacgtttt caccggtgga tgcgcagacg
    17401 gtggaacaac tgaagatgac cgctgaaatt gtctgttcgg tgttccgtgt cccggcctac
    17461 aagattggcg tgggacaacc gccttccagt gacaacgtgg aggcgctgga gcagcagtat
    17521 tattcccagt gcctgcagac actgattgag tccattgagc tgttactgga tgaggcgctg
    17581 gaaacggggg aaaacgagag tacggaattt gatgtcacca cgctgctgag aatggacagt
    17641 gagcggcgca tgaaaacgct gggggatgcg gtgaaaaata cgcttctcac gcccaatgaa
    17701 gcccgtaaac gggagaacct gccgcccctg gcaggaggcg atgcactgta tcttcagcag
    17761 cagaactaca gtctggaagc gttgtcccgc cgtgatgccc gtgaagatcc gttcgcgtcg
    17821 tccggtaaaa cagcttccgt gcctcaggcg gtcgctgcat ctgacggtaa taaggcaatc
    17881 actgaaacag agcatgacgc ggtgaaggcg atgttcaggg ggattctgaa aaaatgaatg
    17941 aacgtgaact gtccattatc cgtgcgctgg gtgaagaatt ttccgcagtg ctggcggatt
    18001 tacagcgcac atttgagggg aaaatagccg cgcaggcaca aacgtttgaa gaaaaactgg
    18061 cttccctgtc tgtggtatta cagaagtgcg tgacgggcga tgatgtgcgt ccgatgcttg
    18121 agcagatggt gaaggaggcg gtaagccata tccctgttcc gcgcgacggt cgtgactatg
    18181 atcccgatgt tctgcagaag gcggtgaatg atgcggtcgc aaatattccg cagccggcag
    18241 acggtaaaag tatcaccccg gatgatgtgc gtccgatgct tgaacagatg gtgaaagagg
    18301 cgctaagcca tatccctgtt ccgcgtgacg gtcgtgacta cgatccggaa gtactgcaga
    18361 aggcggtgaa tgatgccgtc gggaaaatac cggtaccggc ggacggtaaa agtatcaccc
    18421 cggatgatgt gcgtccgatg cttgaacaga tggtgacaga ggcagtgagc catattcctg
    18481 ttccgcgtga tggtcgtgac tatgatccgg aagtactgca
//
LOCUS       DAZGXZ010000100        18334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000100 DAZGXZ010000000
VERSION     DAZGXZ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.100"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..575
                     /locus_tag="ACHKJF_003636"
                     /pseudo
     CDS             1..575
                     /locus_tag="ACHKJF_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012896819.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            678..1181
                     /locus_tag="ACHKJF_003637"
     CDS             678..1181
                     /locus_tag="ACHKJF_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP0950923.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1248..2705)
                     /gene="nuoN"
                     /locus_tag="ACHKJF_003638"
     CDS             complement(1248..2705)
                     /gene="nuoN"
                     /locus_tag="ACHKJF_003638"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP0950924.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLI"
     gene            complement(2712..4241)
                     /gene="nuoM"
                     /locus_tag="ACHKJF_003639"
     CDS             complement(2712..4241)
                     /gene="nuoM"
                     /locus_tag="ACHKJF_003639"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP0950925.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4405..6246)
                     /gene="nuoL"
                     /locus_tag="ACHKJF_003640"
     CDS             complement(4405..6246)
                     /gene="nuoL"
                     /locus_tag="ACHKJF_003640"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP0950926.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEIISGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6243..6545)
                     /gene="nuoK"
                     /locus_tag="ACHKJF_003641"
     CDS             complement(6243..6545)
                     /gene="nuoK"
                     /locus_tag="ACHKJF_003641"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP0950927.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMHG"
     gene            complement(6542..7096)
                     /gene="nuoJ"
                     /locus_tag="ACHKJF_003642"
     CDS             complement(6542..7096)
                     /gene="nuoJ"
                     /locus_tag="ACHKJF_003642"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP0950928.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7108..7650)
                     /gene="nuoI"
                     /locus_tag="ACHKJF_003643"
     CDS             complement(7108..7650)
                     /gene="nuoI"
                     /locus_tag="ACHKJF_003643"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP0950929.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7665..8642)
                     /gene="nuoH"
                     /locus_tag="ACHKJF_003644"
     CDS             complement(7665..8642)
                     /gene="nuoH"
                     /locus_tag="ACHKJF_003644"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP0950930.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8639..11365)
                     /gene="nuoG"
                     /locus_tag="ACHKJF_003645"
     CDS             complement(8639..11365)
                     /gene="nuoG"
                     /locus_tag="ACHKJF_003645"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP0950931.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFRSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11418..12755)
                     /gene="nuoF"
                     /locus_tag="ACHKJF_003646"
     CDS             complement(11418..12755)
                     /gene="nuoF"
                     /locus_tag="ACHKJF_003646"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP0950932.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12752..13252)
                     /gene="nuoE"
                     /locus_tag="ACHKJF_003647"
     CDS             complement(12752..13252)
                     /gene="nuoE"
                     /locus_tag="ACHKJF_003647"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP0950933.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13255..15057)
                     /gene="nuoC"
                     /locus_tag="ACHKJF_003648"
     CDS             complement(13255..15057)
                     /gene="nuoC"
                     /locus_tag="ACHKJF_003648"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP0950934.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15151..15813)
                     /gene="nuoB"
                     /locus_tag="ACHKJF_003649"
     CDS             complement(15151..15813)
                     /gene="nuoB"
                     /locus_tag="ACHKJF_003649"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP0950935.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15829..16272)
                     /gene="nuoA"
                     /locus_tag="ACHKJF_003650"
     CDS             complement(15829..16272)
                     /gene="nuoA"
                     /locus_tag="ACHKJF_003650"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP0950936.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16903..17841)
                     /gene="lrhA"
                     /locus_tag="ACHKJF_003651"
     CDS             complement(16903..17841)
                     /gene="lrhA"
                     /locus_tag="ACHKJF_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP0950937.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4580 a         5085 c         4735 g         3934 t
ORIGIN      
        1 ctgggtcact gcattatcgc caatgccccg acacgtaagt cctgagcaat tagaaaaaac
       61 agttcgttat cttacacaaa ctgtgcatcc acgtaatgcc ggcaatattg ataatctgaa
      121 taggtccgta gagtatataa aagaggtctt tgtcagtagc ggtgccagag ttaccgcgca
      181 gggcgtcccc gattggctat tacgactctg cgcctggaag ccagaattac ccttacccgg
      241 caatgtcctg gctttatccc gatcggggag actttattgc cgtagtcggc agaatgcagg
      301 atatcaacgc cgttcgtcag gtaaaatcgg cattgttgtc atcccgggat ttatctgttt
      361 attctatgaa tgccccaggg tttattcccg gtattgattt ctctgatcac cttaataatt
      421 ggcaacacga tattcccgcc gtaatgatta ctgacaccgc tttttatcgt aataaacaat
      481 accacttgcc cggtgatacc gcagacagat tgaattatca gaaaatggct caggtggtgg
      541 atggtgttat aaacttgtta tacaacagta aataataaaa atttctcgag gcgggtccag
      601 aaaaagcgac atgcacattt tattttccct cgtctgtttt aagatgcgaa acatgaaatt
      661 caatggagga aaaataaatg ggaatgattg gctattttgc agaaattgac tcagaaaaga
      721 ttaaccaact gcttgagtct actgaaaaac ccttaatgga caatattcat gacacgcttt
      781 caggattacg ccgactggat attgacaaac gctgggattt tttacacttt gggctgacag
      841 gcacctctgc ttttgatccc gcgaagaatg atccgctgag tcgtgccgtg cttggtgaac
      901 acagcctcga agatggcata gatggcttcc tgggattgac ctggaatcag gagttagccg
      961 cgacaatcga ccgactggaa tcactggatc gaagtgagct gcgtaaacag ttttccatta
     1021 agcgactcaa tgaaatggag atttatccgg gagtgacctt ttcagaagag ttagaggggc
     1081 aactctttgc ttcgatcatg cttgatatgg aaaagctgat tagcgcgtat cgacgtatgc
     1141 tgcgacaagg gaatcatgcg ctgacggtta ttgttgggtg atggtgtcag ggcaccaaaa
     1201 aaaaccgccg aacatgtcgg cggctttctg acttacaaac tgatagctta gatcagcggc
     1261 attgctaacc gcacaatgct aatcagcggt tgtggccata cacccagcac cagtaccaac
     1321 agtgcagaga tcagcaccac gataccgcct gcgctgtact gccagtttga cggtgcatcg
     1381 cgacccggtt gttccggggc gtgcaggtac aggctcaccg ccacgcgcag gtagtagtaa
     1441 aggccgattg ccgaaccgac aaccacggcg cccaccagcc accacaagtg tgcctggaca
     1501 ccgaccgcca gcacgtagaa cttaccaata aagcccagcg tcatcggaat accggccaga
     1561 gacagcatca tcaccgtcat caccgccgcg aggatcgggc gatgccagaa cagaccgcgg
     1621 taagagaaca gggaatcagc atccgggcca cgatacgggc tggacatcag gctgaccacg
     1681 ccgaacgcgc cgaggctgct gaacagataa ccggccaggt aaacccctac cgcttccatc
     1741 gacatctcac cagtttgcag cgcaatcagc gccaccagca gatagccgag gtgagagata
     1801 gatgagtaac cgagcagacg tttgatgttg gtctggctca gcgccatcag gttaccgaag
     1861 atgatggagg caaaggcgat aatcgccagt actacgcgaa tcgcttcgct gtcacccacc
     1921 ggtgcgtaga ggaacagacg catcaccaca ccgaagatag cgattttgct cgccgtcgcc
     1981 aggaaagtgg aaaccggcgc aggcgcgccc tggtatacgt ctggcgtcca caggtggaac
     2041 ggcaccagcg agagtttgaa gccgaggcca acaatcatca gtccgaaacc tgccagcaac
     2101 agcggctcgt tgagcatacc gtcgccgagg tttttaccca gcgcgacaaa cgacaggtcg
     2161 ccagactgtg catacaccag cgccatacca aacagcagga aagaagacgc tgcggcagaa
     2221 aggatggtgt atttgatact ggcttccagc gaacgtttct ggcggaaagc gtaaccgacc
     2281 aggccaaaca gcggcaaaga gatcagttca ataccgagga acaaagacgc cagatggttg
     2341 gcattcgcca gcaggatccc gcccagcgcg gcaattaaca ccaacaggta gaactcatcc
     2401 ttgttgtcgt tatagccttc aagccacggg taggcgaaag tacaggtggc gaggctcgcc
     2461 aacaatacca gcccggtgta aagcatggcg aaaccatcaa cgcgcatcag cggcgtaacg
     2521 tccatagcgc ccgcctggcc gacaaaccag agcgaaacca gcgccgcgtt aagcccaata
     2581 accgagagcg tagcgttgag gaaatgattg cgtcgccacg caatggagag catcacaacc
     2641 accaccgtca agccgacgat cagcaacggt agcagtgcga tcaggttttg tggagttatt
     2701 gtcatggcga tttacggcct tgtagtagta acggaattaa caaaccactg ctggatattg
     2761 ccaatcgcgg agtgcgaggt atccagaatc ggctgcggat agaagcccag cagtaccagc
     2821 agcaccacca gcaacaggat cataaacagc tcacgcagcg acatccctgg cagttcctgg
     2881 ctggcaatct ggcttttcgc tttaccgaag taagcgcgat gcaacatcgc cagcgaataa
     2941 acagatgcaa agaccagccc aaaggtagag ataacggtaa tcaccgggac aacctggaag
     3001 ctgccgaaca gaatcataaa ttcgccgacg aagttaccgg tgccaggcat cccaagcgtt
     3061 gccaccgcaa agaacagcga cagtgctggc agccatttca tcttgctcca cagaccgccc
     3121 atcatgcgca tgtcgcgggt atggatacgt tcataaagct gaccacaaag aataaacaga
     3181 cccgccgccg acaagccgtg cgcaatcatc tggattaccg cgccctggta ggccaactgg
     3241 ctgccggtgt agatagcaat cagcacgaag cccatgtggg aaaccgaggt gtaggcgatc
     3301 agacgtttga tatcggtctg ggcgaaggcc atccacgcac cgtagaagat gccgataaca
     3361 cccagccaca tagcaattgg cgcgaactct gccgacgcgt tcgggaacag cggcagggag
     3421 aaacgcagca aaccgtaagc ggcagttttc agcaagatcc ccgcgaggtc aacggaaccg
     3481 gcggtcggag cctgggagtg cgcatccggc agccagccat gcagcggaac caccggcatt
     3541 ttgactgcga aggcgatgaa gaagcccagc attaacaggt attccacacc actggacatt
     3601 ggcgtattca gcagctcttc atagttgaag gtccagacgc cggtcgcatt gtagtgaaca
     3661 aaaaccagcg ccaggatggc aatcaacatc accagaccac tcgcctgggt gtaaatgaag
     3721 aacttggttg ccgccgtgat acgcgtttta ccgtcagagg ctttatgccc ccacagtgcg
     3781 atcaggaagt acatcggcac cagcatcatt tcccagaaga agaagaacag gaacatgtcg
     3841 atggcaagga acacgccgat aacgccgccc aggatccaca tcaggttgag gtggaagaag
     3901 ccctgatatt tttcgatctc tttccacgaa cagagtaccg ccagcacacc gagcagaccg
     3961 gtcagcacga ccatcagcag cgacagcccg tcaatggcga gatggataga gataccaaaa
     4021 cgcgggatcc acggcatgtc gaattcagac tgccactgcg gaattccggc ggattgcgtc
     4081 agtgaataac cgccctgcaa ccacagttgc agcgacagcg ccagcgtcaa tcccatggtg
     4141 atcagcgcga tccagcgcgg caccttgacg ccaaagcgtt cggtctgcca gcacaggaag
     4201 ccgccaataa agggaattaa tattagccag ggtagtaaca tggcgatctt tattccttgt
     4261 aaaagtcccg tcaggaccgg attttcaacg aattctcacg acagaaaatt ctcttcggac
     4321 tgggggtagt gtcggatgcg ccgcgaaggc agcttatccg acctacgttc ttatatgccc
     4381 aggggcaatc ccacaatcct taactcaacg cagtaccatc aacagtgcca gcaccacgac
     4441 cgcaccgatg ctcatggatg ccacatacca gcgcagataa ccgttctcgc ttaacagcag
     4501 gcctttacct gcaaagcggg aaagaaccgc cgggatgttc atcattgagt tcagcggatc
     4561 gcgtttcagc aaccaggcaa tacccagaaa cggcttgacg aacactttgt cgtacagcca
     4621 gtcaaagccc caggcgttgt accaccaggt actcagcagg cggcccggcg cactgttggc
     4681 gatggaggtc accagagtac gtttacccag ccacagccag gctgccagca gaatgccgac
     4741 caccgcgacc acgccagaga taatttccag ggtcaacatg ctgccgtgcg ccagttccgt
     4801 cgtttgcgga agcacgccct gcagcggcgg tacaatcagt gcgccaacga aggtggaaag
     4861 gatcagcagc acaatcagcg gcaggctgtg agttacccct ttcacggcgt gagcgtgaat
     4921 ttgttctttt ccgtggaaga cgatgaaaat catacggaag gtgtagagcg aggtcataaa
     4981 cgcaccgacc aaacctgcca ccatcagatt gatatgacca ttcgccatcg cacccgcgag
     5041 gatctcatcc ttactgaaga agcccgcagt gaccagcggc agtgccgaca gtgctgcgcc
     5101 gcccaccagg aagcagagat aaaccagcgg aatagattta cgcagaccgc ccatcttgaa
     5161 gatgttctgt tcgtgatggc aggccagaat gacggaaccg gatgccagga acagcagcgc
     5221 tttaaagaac gcgtgagtca tcaagtggaa aatcgccgca tcccatgcct gcacgccaag
     5281 cgcgaggaac atgtagccaa tctggctcat ggtagagtaa gcgagaacac gtttgatgtc
     5341 ggtctgtacc agcgcggcaa aaccggccag cagcagcgta accgccccga caatgcccac
     5401 cagatgcaga atttccggcg tcatcaggaa caggccgtgg gtacgggcga tcaggtagac
     5461 acccgcggtt accatggttg cggcgtggat cagcgcggag acaggcgtcg ggcccgccat
     5521 cgcgtcggca agccatgtct gcaacggcaa ctgcgcagat ttaccgaccg caccgcccag
     5581 cagcatcagt gtcgcccaca tcagcatatt attgccatca gcaaagtgcg ctggtgccag
     5641 ttccaccatt tcgcggaagt tcagggtgcc cagttcgttg taaagaatga acagtgcgaa
     5701 agcgaggaaa acgtcaccca cacgggtcac gacgaacgct ttcattgccg ctgcgccatt
     5761 cttcggatcg gtgtaataga acccgatcag cagataggag cacaggccca cgccttccca
     5821 gccgaggtac atcagcagca ggttgtcggc aagcaccaga accaccatgc tggcgatgaa
     5881 caggttggtg taggcgaaga agcgagagta gccttcttca ccgcgcatgt accaggaggc
     5941 gtacatgtga ataaggaaac ccacaccagt gaccaccgag agcatggtca gcgacaggcc
     6001 gtccagcacc aggttaaaac cgatgttaaa gtcgcctacc gacatccacg tccacagcgg
     6061 ctggctgtat gcctgctcgc cgttagcgaa gaaatcaacg ccgataaagg cggttaccag
     6121 cgccgccagg cccacagagc caacgccgac gatcgccgaa acgttttcag accagcgccc
     6181 acgggagaac gccagcagga cgaagccaat caatggcaaa ataatggtta aggcaagcat
     6241 gttcatccgt gcatctcact tactgaatcg atgttcaggt tctggcgacg acggtgaagc
     6301 tgcagcagca gcgcaaggcc gatactcgct tctgccgccg cgaggctgat ggcgagaatg
     6361 tacatcacct gaccgtcggt ctggccccag tagcttccgg cgaccacaaa ggccagcgcg
     6421 gaggcgttaa tcatgatttc cagaccaatc agcataaaca gcagattgcg acggataacc
     6481 agaccggtta agccaagaac gaataagatt gccgcgagga tcagtccatg ttgtaagggg
     6541 atcatgcgtg ctcctccgtt tttcttttcg cgctgtcgtc tttacgattg ctcagcactt
     6601 cacccgctcg ctcttcacga ccgacgtgga aggccacaac cagacccgcg agcagcagca
     6661 tagaagccag ttccaccgcc agtacgtaag gcccgaacag cgtaataccc actgctttag
     6721 cactgattgg cgtaccgtcg ataccctgat cattaacacc aaggatggcg taaacaatca
     6781 ccaccagcat gatggccgac aaaattgccg gaccaatcca cacctgcggt ttcagccact
     6841 ggcgttcctg ttcgatttct gaaccgccca ggttgagcat catcaccacg aacacgaaca
     6901 gcaccataat ggcacccgcg tagacgataa tttccagcgc accggcgaag taagcgccca
     6961 gtgagaagaa caccccggaa atcgccagca gcgaaataat caggtacagc agtgcgtgta
     7021 ccggattggt atgggtgatc actcgcaagg tcgcaagtat ggctatcagg ccacagatat
     7081 aaaaagcgaa ctccattgcc cctctcctta cggtaacagg ctcttgacgt cgataggctt
     7141 ggcttcgttc tctgcttcgc ccttatcttt gccgtcgatt gccatacctg ccatccggta
     7201 gaagttatat tccgggtatt tgcccggacc ggagatcagc agatcctctt tttcgtaaac
     7261 cagatcctgg cgcttgtatt cccccatttc gaaatccggg gttaactgaa tcgccgtggt
     7321 cggacaggct tcttcgcaca gaccacagaa aatgcagcgt gagaagttga tgcggaaaaa
     7381 ttccggatac cagcgaccgt ctttggtttc tgctttttgc agcgagatac agccgaccgg
     7441 gcaggctacc gcgcagaggt tacaggctac gcaacgctct tcgccgtccg ggtcgcgggt
     7501 cagaacgata cgaccacgat aacggggcgg cagatagacc ggctcttccg ggtacattcg
     7561 cgtttcgcgt ttggcgaacg cgtgcaggcc gatcatccag atactacgaa cctgggtgcc
     7621 gaaacctact aacaattctt ttaaggtcat ggtcttattg ccccttattg cgcctgccag
     7681 agaatgacag ccgccgttac cagcaagttg atcagcgtca gcggcaggca gattttccag
     7741 ccgaaggaca ttacctggtc ataacgcgga cgcggtaacg acgcacgaat caaaatgaac
     7801 atcatcataa agaacgcggt tttcagcgcg aaccagatga atggcggtaa caacgggcct
     7861 tgccagccac cgaagaacag cgtcaccatc aatgcagaga tggtcacaat cccgatgtac
     7921 tcacccacga agaacagacc gaacttcata ccggaatatt caatgtggta accatccgcc
     7981 agttcctgct cggcttcggg ttggtcaaac gggtgacggt gacacacagc cacgcccgcg
     8041 atggcaaagg taataaaacc aaagaattgc gggatgacgt tccacacatg cgcctggcta
     8101 ttgacgatgt cggtcatatt gaatgaaccg gcctgcgcca ccacgcccat cagggaaagc
     8161 ccgaggaaca cttcgtagct cagagtctgc gcagaagcac gcatcgcacc gagcagcgag
     8221 tatttgttgt tacttgacca gcccgcaaac agcactgcgt aaaccgccag accagccatc
     8281 atcaggaaga acaaaatccc gatgttcagg tcggcaacca cccaacccgg actgactggc
     8341 acaatcgcaa aggccagcag cagtgaggta aaggcaatca tcggtgccag ggtaaagatg
     8401 acgcgatccg agaatttcgg gatccagtct tctttaaaga acattttgat catgtccgca
     8461 accagctgga gcgaaccgcc ccaaccaaca cggttaggtc cgtaacggtt ctggaacaga
     8521 cccagcaggc gacgttcgcc aaagctcatg aatgccccgc aggtgacaac caccagcagg
     8581 atcaccaccg ctttgaggat ggtcagcagg atctcaatca gttccggtga tatccaactc
     8641 attgttgtgc ctccttgaga tcctcaagat gcgcgccagc cagcaccgga gcaatgccgg
     8701 acatacccat cggcaagccc acctgccctg ccgtcagccc ttcggcgatt tcaaccggca
     8761 gcgtgaccgt gttgccatcg taactaaagg agacgcgtgt acctgcgttc acacccaact
     8821 tcgcggcatc cgctgggttg agtttgatgt acggctgcgg catacggctc cggaagaccg
     8881 gagcacgctg tgacaattca tcgctgccaa acaggtggta gtacggcgcg atacgccatt
     8941 tcccatcctg cggctggaag cgtgccggta cgctggtgaa gtaatccaga ccgttttcgc
     9001 tggtttcaaa cagacgcacg cccggatcgc caaagcgcag tttgccgccc acttcgtcct
     9061 ggaatttgtt ccacgcctgc ggggagttcc agcccggcgc ccaggcaaac ggcacttgcg
     9121 aacggtgcgc ggtcggctgg ttgttacctt ccatcgagaa ggtgaacatg gtgtcaatat
     9181 cctgcggctg acgcggctca tgaacgctga tattggcgcg catggcggta cgaccgctgt
     9241 aacggtgcgg ttcacgggcc agtttctgcc cacgaatacg gaatgtcgca tccggcgcag
     9301 catctttgat acctgccagt tccgggattt tcgccacaac agcgtcaatt acatggtcga
     9361 gctgcgtcca gtccacttca cggctcagca gagtgctgtg cagcgagtgt aaccagcgcc
     9421 agctttccag catgacagtt ttgctgtcgt aataagcagg atcgtaaacc tggaagaaac
     9481 gttgggcgcg gccttcgttg ttgatcaccg taccgtcgct ttcagcaaag ctggcagcag
     9541 aaagtaccag atgggcgttt tccataatcg ctgtgcgttg atgatcaacc accatcacca
     9601 gcggcgcttt agccagcgca gcattcacgc gggtagcgga agcgtgacga tgcagatcgt
     9661 tctccagcac caccaccgcg tcggcgcgtc cggtttccag ttcggttaac gcttcttcaa
     9721 gcgatccacc gcccataatg cccagcccca tgctgttgac ggaacgggca atcatggtga
     9781 taccgacgtc agcgccgcga cctttcaggg ctttcgcgac gttagccgca gcctgaatta
     9841 cttcagcgct accggcgttc gtcccggaga taatcaacgg tttcttcgca ccagccagtg
     9901 cctgcacgat gacgtcgatt ttgctttgca gctcaggttc gataccgtca accgctggtg
     9961 cagagttatc cagcgcatgg gcgatggcaa aacctaaacg cgcctgatct tcaaccggtg
    10021 cgcggtaagt ccacgccgcg atatcatcca gacgggtgtc atcaacgttg gtaacaaaca
    10081 gcggatgctt cgcacgttga ccgatgttga ggattgccgc aatctgccag tcagccactt
    10141 tctgtgctgc cgccatttcg cgcgctttac ctttcaccgc ctgacggact gccagcgcga
    10201 cgcgcgcgcc ggtctgggta acgtcttcgc ccagcaccag taccgcatcg taagattcga
    10261 tttcgcgcag agccggagta taaatgccgc cttcacgcag cactttcagc gccagttgca
    10321 gacgttcctg ctcaccgtga gcgataccgg tgtagaagtt ttcttcgccc accagttcac
    10381 gcagcgcaaa gttgctttcc acgctggcac gcggagaacc aataccgatc actttcttcg
    10441 actgacgcag aatatctgcc gcgccctgca ttgcctgttc ggcgttgagg gtaatgaaat
    10501 catcgccacg acgctgtact ggctgacgcg gacgatcttt caggttgacg taaccgtaac
    10561 cgaaacgacc acggtcgcag aggaagtagt ggtttaccgt accgttgtaa cggttttcga
    10621 tacgacgcag ttcgccgtaa cgttcaccgg ggctgatgtt acagccgatg gaacattgct
    10681 ggcagatgct cggcgcaaac tgcatatccc atttacggtt gtaacgctcg gagtgcgttt
    10741 tgtcggtaaa tacgccggtc gggcaaattt cgaccaggtt accggaaaat tcgctttcca
    10801 gcgtgccgtc ttccgggcga ccgaagtaga cgttgtcgtg cgcgccataa acgcccagat
    10861 ctgtaccgtc agcgtaatct ttgtagtaac gcacgcagcg gtagcaggcg atgcagcggt
    10921 tcatttcgtg agagatgaat ggccccaaat cctgattacg gtgggtacgt ttggtgaaac
    10981 ggtagcgacg gaagctgtgt ccggtcatca cggtcatatc ctgaagatgg cagttaccgc
    11041 cttcttcaca taccggacag tcgtgcgggt ggttggtcat taaccactcg accacacttt
    11101 cacgaaactg tttcgcttct tcgtcgtcaa tggaaataaa ggtgccatcg gaagccggtg
    11161 tcatacagga catcaccagg cgaccacgcg tgtcttccgc gttttggtat tgctttaccg
    11221 cacactggcg gcaagcaccg acgcttccca gcgccggatg ccagcaaaag taaggaatat
    11281 caaggcccag agacagacaa gcttccagca ggttgtccgc tccgttgacc tcgtattctt
    11341 tgccgtctac atgaattgta gccattagca tgcttccagt tttctcagtc agagactgag
    11401 cgttaatcga aattcggtta ccagcgctct ttcagcaggt tcggctgaat cccattaatc
    11461 aaatgggtat tgctgaacgg ctgtttgatt cccgcctcaa attcttcgcg gaaatatttg
    11521 atggcgctct gtaacggctc cactgcacca ggtgcgtggg cacagaaagt tttacccggg
    11581 cctaagaatc gacacagttg ctcaagtgtt tcgatatcgc ccggctgacc ttcaccacgc
    11641 tccagcgcac gcagaatttt cacgctccac ggcagaccgt cgcggcacgg cgtacaccag
    11701 ccgcaggact cacgggcgaa aaactcttcc aggttacgca ccagcgacac catgttgatc
    11761 tcatggtcaa ccgccatcgc cagcgccgta cccagacggc ttcccgcttt accgatactt
    11821 tcgaattcca tcggcaggtc aaggtgcgct tcggtcagga agtcggtgcc cgcgccgcct
    11881 ggctgccagg ctttgaattt cagaccgtca cgcataccac cggcgtaatc ttcgaggatc
    11941 tcgcgcgcgg tagtaccaaa cggcagttcc cacaggcccg gatttttcac ccgaccggag
    12001 aagcccatca gcttggtgcc agcatcttta cttttcgaga tgttctgata ccactccacg
    12061 ccgttagcga ggatcgccgg aacgttacac agggtttcga cgttgttgac acaggtcggt
    12121 ttgccccata cgccggaggt tgccgggaag gggggtttcg agcgtgggtt agcacgacgc
    12181 ccttccaggg agttgattaa cgcggtttct tcaccgcaga tgtagcgccc tgccccggta
    12241 tggacgaaca attcgaaatc aaaacctgtt cccataatgt ttttgccaag cagacccgct
    12301 tcggtggctt cggcaatggc acggcgtaga ttaactgccg cttcgatata ttcgccgcgc
    12361 aggaagatat agccacggta agctttcagc gcaaacgcgg agatgagcat gccttccacc
    12421 agcaggtgcg gcagttgctc catcagcagg cggtctttat aggtgcccgg ctccatttca
    12481 tcggcgttac acagcaggta acggatgttc atggattcgt ctttcggcat cagactccac
    12541 ttcaggccag tcgaaaagcc cgcgccgccg cgccctttca gaccagcgtc ttttacctga
    12601 ttaacgattt cgtctggaga cagcccggtc agcgccttac gcgcgccttc gtaaccgttt
    12661 ttgctgcgat attcgtccag ccacacaggc tgtttgtcat cgcgcagacg ccaggtcagc
    12721 ggatgcgttt cgggagtacg gataatgttt ttcatttata ccgctccagc agttcaggga
    12781 tcgcttccgg ggtcagatgc gcgtgagtgt cctcatcgat catcatattt ggccctttat
    12841 cacagttccc caggcagcag gttggcagca gcgtaaagcg accgtcaaac gtcgtttgcc
    12901 ctggtttgat gttcagtttt ttctccagcg ccgcctgaat accctggtaa ccgttgatgt
    12961 gacagaccac gctgtcgcaa taacggatca catggcgacc aaccggttgg cggaagatct
    13021 ggctatagaa cgttgccacg ccttcgacat cgctcgccgg aatacccagc acatcggcga
    13081 tcgcgtggat cgcgccgtcc ggcacccagc cacgctgctt ctgaacgatt ttcagcgctt
    13141 caatggacgc cgcacgcggg tcttcgtagt ggtgcatctc gtgctcaatc gcttcacgct
    13201 ctgccgcact cagctcaaaa gcctcggttt gtggttgttg attctcgtgc ataattagcg
    13261 gtccacatct gacataacaa aatcgatact gcccagataa acaatcaggt cagacaccag
    13321 gctgccgcgg atcgccgccg gaatttgctg caaatgcgca taactcggcg tgcggatacg
    13381 agtgcgataa ctcatggtgc taccgtcgct ggtcaggtag taactgttga tccccttggt
    13441 cgcctcaatc atctggaaag attcattggc aggcatcacc ggcccccacg acacttgcag
    13501 gaagtgggtg atcagggttt cgatatgttg cagcgtgcgc tctttcggcg gcggcgtggt
    13561 cagcgggtga tccgctttga acgggccttc cggcatgttg ttgaggcact gctcaagaat
    13621 gcgcagactc tggcgcagct cttccacttt cagcattacg cgggtgtagc agtcagaaac
    13681 accaccacca accgggattt caaagtcgaa gttttcatag ccagaataag gacgcgcctt
    13741 acgcacgtcg aagtcgatcc cggtagcacg caggcccgcg ccagtggtgc cccactccag
    13801 cgcctctttc gcgccatagg cggcaacgcc ctgggaacga cctttcagaa tggtattttg
    13861 cagcgccgct ttctcgtaag acgccagacg tttcggcatc cagtcgagga actcacgcag
    13921 cagacgatcc cagccgcgcg gcaggtcgtg cgctacgccg ccaatacgga accacgccgg
    13981 gtgcatacgg aaacccgtga tcgcttccac cagatcgtaa attttctgac gatcggtaaa
    14041 ggcgaagaac acgggcgtca ttgcgccgac gtcctgaata aaggtcgaga tatacagcag
    14101 gtggctgttg atgcggaaca gttcggagag cattacgcga atgacgttaa cgcgatccgg
    14161 cacggtgatc ccggccagtt tctctaccgc cagcacgtaa ggcatttcgt taacgcagcc
    14221 gccgaggtat tcgatacggt cggtatacgg aatgtagctg tgccaggact ggcgttcgcc
    14281 cattttctcc gcaccacggt ggtggtagcc gatgtctggt acgcagtcga caatctcttc
    14341 gccatcgagc tgcaaaacga tacggaaagc accgtgcgcc gacgggtggt tcggaccgag
    14401 gttgaggaac atgaagtcct cgttttcggt gccgcgcttc atcccccact cttccggttt
    14461 gaaggtcagg gcttccatct ccagatcctg tttggctttg gtcagctcaa acggcgagaa
    14521 ttcggtagcg cgcgccgggt aatctttacg cagcgggtga cctttccagg tttgcggcat
    14581 catgatgcgg cgcaggttcg ggtgaccgtc gaaagtaatg ccaaacagat cccaggtttc
    14641 acgctcatac cagttagcgt tcgggaacag tttggtgaag gtcggtacgt gcaggtcgtt
    14701 ttctgccagc gccaccttca gcatgatgtc gcggttacga tcgatagaaa tcagatggta
    14761 gaaaacggaa aaatccgcgg caggtaaccc ttcgcggtgt gtgcgcagac gttcgtccat
    14821 gccgtgtaag tcaaacagca tgacgtaagg tttcggcagt ttctttaaga aatcgcccac
    14881 ttccagtaat tgttcacgct tgatccacac aacgggaacc ccggtgcgag tcgcctgaac
    14941 agtaaaggca tccggcccaa aacggttgcg cagttcgcca atcaccggat catcaagatg
    15001 atcgcgggtc tgccaggcgg gttcttgcgc ggttaagtcg gtcatattgt tcaccattgc
    15061 aaatggtccg tggtgactgt cggcgcagta agcttcgcga aatagattta ggaataagcg
    15121 aagaaaaatc cctttgccga caggcgcaaa ttaaatctcg tcaggtgtac gcaggttggt
    15181 tacggcaatg cgttcaccgc gcttgcgttc gcgctctgat tgcatattgg cgcgataaac
    15241 gccctgatcg ccaaccaccc aggagagcgg acgacgttct ttgccgatag attcctgcaa
    15301 cagcatcagt gcctgcatgt acgcttcagg acgcggcggg cagcccggga tatacacatc
    15361 aaccgggatg aatttatcga cgccctgcac aacggaataa atatcgtaca taccaccaga
    15421 gttggcacag gcacccattg agataaccca ttttggttcc agcatctggt catacagacg
    15481 ctgaataacc ggtgccattt tggtaaagca ggttcctgcc actaccatca ggtcagcctg
    15541 acgcggcgaa gcacgcaata cttctgcgcc aaaacgcgcc acgtcatgca ccgcggtaaa
    15601 cgaagtcacc atctcaacgt aacagcagga aagaccgaag ttatacggcc aaattgagtt
    15661 tttacgaccc cagttaacca tgtcattgag cttgcccata aacacgtttt tgttaacttc
    15721 ttgctccaga gggtcggtta cgatctcctg cttttgcagg gggtaacggt cgttctcacc
    15781 gttgggatct atgcgggtga gcgtataatc catcttaatg cctcgcggtt agcgttgacg
    15841 attagcgata ctgttcgttt ccgggttcat acgctcgcgg cgtgaacgcg cgggcgtcca
    15901 gtccagcgcg ccaatacgca ccagataaac cagacctgcc agtaacacaa aaataaaaat
    15961 tgcagcttcc acaaagccta cccagccgct ttcgcggata gaggttgacc atgcgaacag
    16021 atacagcgct tcaacgtcga agataacgaa gaacatggcc accagataaa acttggcgga
    16081 caggcgtaag cgggcggagc cgaccgagtc gataccggat tcaaacggca cgtttttcga
    16141 cctcgcgcgt gcgcgaccgc ctaaaaacca accgcctacc agcatcaggc aacacaggcc
    16201 aatggcaacg ataagaaaga tagcgaatgc ccagtgatga gcgatgactt cagtggatgt
    16261 tgacatactc attgcttact catcaaaagt agcgccagat tcactgctct tcacggcaga
    16321 tggacgccac atcgattcat ggggaggaat aaaaaaaacc ttacaatcac tgtagaaatt
    16381 cttttataca gctaattgat gtggtctttt actcctttct ataacctttt gtcaacttta
    16441 acaaaagttt cttcacatta gtttacataa tatcaacacc attagcattt aatgcccttt
    16501 caccccagat ccttgacgac tccaggataa ttagatgttg ttgaatcgtg tccgttgtga
    16561 agcaatggaa aaaatacggg tctattttga caggaatttg tgtcgattcc tccccccaaa
    16621 agagagtatt ttcttgatct gtgacacgct tttgtcattc cataacaaaa acgcagcaac
    16681 aaatttacgt attttttaac atcattgtag caggtgattt ttttcaggcg attatttgtg
    16741 cgtacggggc gtgaatctct ggtgattgaa aaatgaacag ttttgtacgt tctgcactat
    16801 gcgaggaagg atttttacta aaaaaaagcc gctggggtct aaaacacccc cagcggctcg
    16861 ttttttacac tattgtctca ggaattatct atcgtccgtc gattactcga tatccctttc
    16921 aatcaacaat gaatcatccc cttccggagc agacattggg ctgtattgcc acggattatg
    16981 gtagctctcc atcgcctgat agatcacctg ggccagctca ttattactgg acgggtcata
    17041 gcacagcaaa tattcggtat caggtagcgg cggtaagcca tctactccac tcagcacacg
    17101 caagtccgga ctcatcatct cgaccggcct tgccgtcacg ccaagaccgg cttttactgc
    17161 cgcacgaact gccggaagcg tcgaggcgac ataagccagt cgccatggaa tatctgcttt
    17221 attaagcgtc gccagcacca tatcgcgaaa cggacttgga tcatccagca atacaagagg
    17281 gatcggctca cctttttgca atacgtattc cgctgcgcag taccagtgtg ttggcgaggt
    17341 acgcaggttc aatgccttaa acgctgaagg acgatgggtg gttaccatca aatccacttc
    17401 ctgggattcc agcatttctg ccatataggc attacgttta acgcggacat ccagcgctaa
    17461 tttcggataa accgaactca cgcgatttaa caggaaaggt aagatcgtat cggcagattc
    17521 atctgaagca ccgatagtta acacgccctg aagattactg aacattaatg atgagcaggc
    17581 ctcatcatta aaacgcagga ttttcctggc gtaaccaaga agttgaatgc catgttcagt
    17641 taacagtttg ttgcgaccgt gacgagcgaa cagttctttc ccaacgagtt gttccagacg
    17701 ctgcatttgc tgacttacgg cggactgagt acgacacaca gcggcagctg cggcagcaaa
    17761 agtgttcaga tcggcaacag caacaaatgt tctcagcaga tcgaggtcga ggttaattat
    17821 cggacgattt gcacttatca tatattatca cttactggcg gctcatactg agctggttaa
    17881 tgctgtgcac acacaaacaa gcaattccat ttgtaatgtg cctccctggc agtttcatcc
    17941 ggaacccgga cgaaagtaaa aatgcatatg agttgcacta aaaaagcgac tcacattgtt
    18001 ccgttataat gcctgaagta gatcacagaa tatatcttca gggatcgcat atctattaag
    18061 ttactcactc ttttctattt atgacatgcg cgtgtttgta taaatgtaag tccttgttcc
    18121 actctcgtgc agcatcgctg gtcatacgcg aacacgtaca aacagcaatg gtgaggcggc
    18181 atcaagagca gggatccgtc atttatgcga gcattttacc ccaaaaacct tttatttata
    18241 agggtcattg cgaattatct gatgcaaagt tatgttatgt taggcaaagt aatcttctat
    18301 tattaataag cacatcaaaa cctttttgaa tatt
//
LOCUS       DAZGXZ010000101        18072 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000101 DAZGXZ010000000
VERSION     DAZGXZ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18072
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.101"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..743
                     /locus_tag="ACHKJF_003652"
     CDS             36..743
                     /locus_tag="ACHKJF_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0950938.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            740..2191
                     /gene="djlC"
                     /locus_tag="ACHKJF_003653"
     CDS             740..2191
                     /gene="djlC"
                     /locus_tag="ACHKJF_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFP0950939.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLAELCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2251..3921)
                     /gene="hscC"
                     /locus_tag="ACHKJF_003654"
     CDS             complement(2251..3921)
                     /gene="hscC"
                     /locus_tag="ACHKJF_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP0950940.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVTRTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDEARECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVIIDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4005..4940)
                     /gene="rihA"
                     /locus_tag="ACHKJF_003655"
     CDS             complement(4005..4940)
                     /gene="rihA"
                     /locus_tag="ACHKJF_003655"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP0950941.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5058..5783)
                     /gene="gltL"
                     /locus_tag="ACHKJF_003656"
     CDS             complement(5058..5783)
                     /gene="gltL"
                     /locus_tag="ACHKJF_003656"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP0950942.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5783..6457)
                     /gene="gltK"
                     /locus_tag="ACHKJF_003657"
     CDS             complement(5783..6457)
                     /gene="gltK"
                     /locus_tag="ACHKJF_003657"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP0950943.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6457..7197)
                     /gene="gltJ"
                     /locus_tag="ACHKJF_003658"
     CDS             complement(6457..7197)
                     /gene="gltJ"
                     /locus_tag="ACHKJF_003658"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP0950944.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7367..8275)
                     /gene="gltI"
                     /locus_tag="ACHKJF_003659"
     CDS             complement(7367..8275)
                     /gene="gltI"
                     /locus_tag="ACHKJF_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP0950945.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8730..10607
                     /locus_tag="ACHKJF_003660"
     CDS             8730..10607
                     /locus_tag="ACHKJF_003660"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP0950946.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLLSSVFLHSSVSHLLMNMFALLVVGGVVERILGKWRLLI
                     VWLFSGVFGGLISACYALRESEQIVISVGASGAIMGIAGAAIATQLASGAGTHHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSFILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10685..12223)
                     /gene="lnt"
                     /locus_tag="ACHKJF_003661"
     CDS             complement(10685..12223)
                     /gene="lnt"
                     /locus_tag="ACHKJF_003661"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP0950947.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12248..13126)
                     /gene="corC"
                     /locus_tag="ACHKJF_003662"
     CDS             complement(12248..13126)
                     /gene="corC"
                     /locus_tag="ACHKJF_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP0950948.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13216..13683)
                     /gene="ybeY"
                     /locus_tag="ACHKJF_003663"
     CDS             complement(13216..13683)
                     /gene="ybeY"
                     /locus_tag="ACHKJF_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP0950949.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13680..14720)
                     /locus_tag="ACHKJF_003664"
     CDS             complement(13680..14720)
                     /locus_tag="ACHKJF_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP0950950.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14873..16297)
                     /gene="miaB"
                     /locus_tag="ACHKJF_003665"
     CDS             complement(14873..16297)
                     /gene="miaB"
                     /locus_tag="ACHKJF_003665"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP0950951.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16443..17618
                     /gene="ubiF"
                     /locus_tag="ACHKJF_003666"
     CDS             16443..17618
                     /gene="ubiF"
                     /locus_tag="ACHKJF_003666"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP0950952.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDITWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPILKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17772..17846)
                     /locus_tag="ACHKJF_003667"
     tRNA            complement(17772..17846)
                     /locus_tag="ACHKJF_003667"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17812..17814),aa:Gln,seq:ctg)
     gene            complement(17884..17958)
                     /locus_tag="ACHKJF_003668"
     tRNA            complement(17884..17958)
                     /locus_tag="ACHKJF_003668"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17924..17926),aa:Gln,seq:ctg)
BASE COUNT         4397 a         4550 c         4707 g         4418 t
ORIGIN      
        1 gtagtcagtt aatgcactta agggattatt aacaaatgaa taaggaagaa caatatcttc
       61 tttttgcgct ttctgcgccc atggaaatac ttaatcaggg ttgcaaaccc gcgcatgact
      121 cgccaaaaat gtataccggt ataaaagaat tcgatctctc atcatcctgg ggaataaata
      181 atcgtgatga tttaattcag actatttatc agatgactga tgatggtcat gccaatgatt
      241 tagctggctt atatctgaca tggcatcgct cttcccctga agagtggaaa gcattaattg
      301 ctggtggctc ggagagaggg ttgatttata cccaattcgt tgcccaaacc gctatgtgct
      361 gtggagaagg gggaatcaaa gcctgggatt atgttcgaat ggggtttctg tcacgggtcg
      421 gtgtgctcaa caactggctg acggaagaag agagtttatg gctgcaatcg cgcgtctatg
      481 tacgagctca ccattactat cacagctgga tgcactattt ttccgcctat tcgttaggac
      541 gtctctactg gcaatcttct cagtgcgaag acaacacttc gctacgcgag gcgctaaccc
      601 tgtacaaata cgatagcgcc gggagtcgga tgttcgaaga gctggcagcg ggaagcgatc
      661 gtttctatgc cacgcttccc tggcagccat taactgtcca gcctgaatgc ccggtaacgc
      721 ttaaggatgt gagcgaccta tgaaaacatg ttggcaaatc ctcgaaattg aaagcacgac
      781 gcaaatagac attatccgcc aggcttatct tgctcgctta ccgttatgtc atcccgaaac
      841 cgatccgcag gggtttaaag cattacgcca ggcctatgaa gaggccctgc gactggcggt
      901 aaatcctgtc ggggaagcag ataatgaaga taaagatgcc gccgctgaac atgaaatact
      961 acgtgcattc aggacattac tggattcaga aagtgatcgt tttcagccct ccgcctggca
     1021 gaaatttatt cagcaattaa atacctggaa catggaggat gtcgatcaat tacgctggcc
     1081 gctgtgtgca atcgctatag aagcgcgata tctttcatta aattgtgctt ctttgctggc
     1141 agagcgtttg aactggcatt cgtttaatga cagcgaagga atggatgagg aagaaaggga
     1201 ggcttttctt gaggccattc aggctggtga ttgtttcgat ttccttagcc ttctggaata
     1261 tcccgtcgcg ttacagaacc agactgttga atattacttc gcgctggaac gttgctgccg
     1321 ttaccatcct gactatgtca ctgcgttttt ggcgatggaa ggtccgtggt tcattcctga
     1381 tgatgcaaag ttacatcgca aactgttgcg ctggtacagc tcggtgcaaa caggtatggc
     1441 ggaactcatt cctgtagcca aacagtggca agcggaagaa ccagaaagcg aagatgcccg
     1501 gtattaccag tgtgcacaac gtttgtactg cggcgagggg gaaagcctgc ttgctgagct
     1561 ctgcgcatac tgggagagtt acccatctac acaagctgat aatttgttgt tgcagtggag
     1621 caagcgtcat tgcccggatt atttcgcgtt attagtgatg gttatcgaag cgcggagcat
     1681 ggtagatgcg caaggtcaac cgctgaaata tgttcctggt gagagcgccc ggacgcggct
     1741 gttatgggcg gaaattttac atagcggaaa attatcgccg ttaggtcaat cgtttattga
     1801 gtcgttattc ttcaaacgca aagcatgggc gtggtggaaa tcgagagtcg gtagcgagac
     1861 agaagaagat tcaccgttac tggatttgta tcgggtagcg gaacaggtag tacttgaagc
     1921 gtttccgaaa caagagatgc tggcccgtct taatacaagg ctggaaggcg gagatgctca
     1981 tccattagag gccattatca cccggttgct gttgacgaaa gtaaaactcg agccggagga
     2041 tgaagatgtc gatgagccaa cacctgaaaa ccatgaagaa aaaaatgatg agggtgaaaa
     2101 accacagagc attaccagca ttatcaaaat cagtttaacg gtgctggtta taggttatgc
     2161 tctcggcaaa atcgcgatgt tgtttagttg acgatgtgtt ttttgcgccg atgccggatg
     2221 cagcatttac gccgcatccg gcagccgtga ttatgaggtc tcaatggcta aataaacgct
     2281 cgcccgccgc ctgacctcat cgacgcgggc catatcgttt gactgcagcg ccgcatcaaa
     2341 atcgatgatc acccggccaa tctcttctcg ctcgtcaccc agcgcccgcg cccacaactc
     2401 ttccagtttg gctttaaagg tgcgattgat gagcatatcg cgcggataaa ttttcaatgc
     2461 ggataaccgc gtccgactct cttcaatctg ctgcgcgctc aatgttaccg ggctgtggtt
     2521 aatcactctg gacttaacgc tgccgtcttc cagaagcacg tcaacttcaa gcaacccgtt
     2581 gatatcataa ctaaagcgaa tatcaatcga ctgataagcc ccggttttct tcaacggcac
     2641 atcgaaggat tccaccagaa tgttgttttt aactttgtgg ttttctccct gatagacgtt
     2701 aaccgtaatt gaatcctgtt ccgggtgcat ggttgaataa gtttctacac gcgacacggg
     2761 cacagtggtg tttcgttcaa taatcggcga gaaaatgccg gaaacgccct ggcggttaac
     2821 ttcaacgccc aacgagtaag ggcaaatatc agtgaggatt acctcttcaa tatcttcact
     2881 gcgtaagcgg caggcggcct ggattgctgc gcccagcgcg acaatggtgc tcggatcgta
     2941 actttgatac ggtaatttgc caaacagacg cacggcgatt cgctgcacca gcggcatttg
     3001 tgacgcgccg ccaaccagca ccagactgtc gatttgactc ggcttcagac gtgcatcgcg
     3061 caacgcctgt tcaatcggca ctcgcaagcg attgagcagc ggcaaccaca aatcttccag
     3121 ttcattctca taaaattcgc attcccgtgc ttcatcctgg tactgccagc gaatgtgtaa
     3181 tggcgattga ttgctacatt ttgccgcttc cacacaggca tacaaggctg ccagttcact
     3241 ctcgttaagc gtggtcctgg tgacatccgc gcgctttaaa acgtcatcga ccaacatatg
     3301 ggtaaaatct tcgccaccga ggaagttgtc gccagcggag gcgtgaactt caatcaccgg
     3361 cgtggcgtac tcaagcaccg taacgtcaaa cgtgccgcca ccgagatcaa acaccagcga
     3421 acgggtattt tgttgggtat gcaggccata cgccatcgca gctgctgtgg gttcattaat
     3481 taagcgtacc gcatttaacc cggctaactc cgctgctaaa cgggtatgct tgcgttgttc
     3541 atcgctgaaa taagccggaa cggagatcac cacatcttta atcggacgtt gcagaaattc
     3601 ttcggcatct tcttttaatg agcgtaatac caaagaggac agttctggcg cgttaaatgt
     3661 gtcgctgcct aaccgccagt tggtattact gcccattgca cgtttaaata acgctgccgt
     3721 tttatccgga tgcgaagtac gccgtgatac agccggtttt ccgactaaaa tatgattatt
     3781 ttcatccatg ctaattatgg atggtgttaa atattcaccg aacttatttg gaattaattg
     3841 cgcggcaccg tctttccaga cggcaattaa actattggta gtaccgagat cgataccaat
     3901 ggcgagttct gcattatcca ttgcatttat atcttgaaag aaaaagagat aaacagatta
     3961 agaccaccca ggtaacgcgt ggtgatctta atcaatgacg tgtgttaagc gtaaaatttc
     4021 agacgatcag ccagtaaatc aacaaagccc tgacgatcaa catcgaccat tacggtggca
     4081 ttcggtttat tgccagtcag ataataataa tcaacaaccg tcataccctg agtatatttc
     4141 ccctgtgttt ccacgccaac ccagcgctca acagtggtaa acaactccgg tttcaacagc
     4201 caggcgatgg tgcatgggtc atgcagcggt gcgccgacaa agccccattt ttcgtcttta
     4261 tgatattcga ggaagaaatc cagcagttcg gcaacaatgg ttgaaacagg gttgccaatc
     4321 gcgcggaaac gctcagtgtc ttcaacgtgg atttgtgctt tgtgagtaac atccagaccg
     4381 gccatcacca ccgggatccc tgactggaag acaatttctg ccgcttccgg gtcaacgtaa
     4441 atattaaatt cagccgcagg cgtccagtta ccaagcccca ttgcgccgcc cataatcacg
     4501 atacgggcaa ttttgctatg cagttccgga tggctattga gcagcaaggc aacgttagtt
     4561 tgcggtccgg tagacacaat cgtgacaggt tccgcacttt cacgcagcgt tttcgccatt
     4621 agctctaccg ccgtacagtt ttgcggtgcg aacgccggtt ccggtaatgc cgggccgtcg
     4681 agaccgcttt cgccgtgcac attgtccgcg ataatcaact cacgcattaa cggttttacc
     4741 gcgccgcctg ctaccggaat atcggtgcga ttgagcaagg tcagcatacg aagaacattg
     4801 cgtaaggttt tttctggtgt ctggtttccg gcggaagacg taattgcttt gacatcaagc
     4861 tctggtgagg cgagggcgag aactattgcg atagcgtcgt catgacctgg gtcgcaatct
     4921 aacagaattg gcagtgccat tgttgctcct tgttgtgtgc ttctttgcga caagggtaac
     4981 gccaggatgt aacagatacg aggggcgaaa cgataaagcg tgagatggcg cgcacttggg
     5041 tatgcgcgcc agagtgatta atgcaggatt ttcgcgagga agtcttttgc gcggtccgat
     5101 ttcggatcat cgaagaaagc gtctttcggc gagtcttcga caattttacc ctcatccata
     5161 aagatcaccc gattcgccac tttgcgggca aagcccattt cgtgggtcac caccatcatg
     5221 gtcattcctt cgttcgccag ttccaccatc acgtccagca cttcgttgat catctccgga
     5281 tccagcgccg acgtcggttc gtcgaacagc atcgcaatag gatccataca caatgcgcga
     5341 gcgatcgcca cacgctgctg ctgaccgccg gaaagctgcg ccggaaattt attggcatgt
     5401 gcggaaagtc ccacgcgctc cagcaatttc aatgcttttt cacgagccgg cgctttatcg
     5461 cgtttaagca ctttcacctg cgccagggtc aggttttcga taatcgacag atgagggaac
     5521 aactcaaaat gctggaacac catcccgact cgggaacgca gctttgccag atcggttttc
     5581 ttgtcgttaa ccacgatacc atcgacggtg atttcacctt gctgcaccgg ttcgaggccg
     5641 ttgacggttt taatcagcgt tgattttccg gaaccagacg ggccgcaaac caccaccact
     5701 tcgccttttt tcacttcggt tgagcagtcg gtcagcacct gaaagtgacc ataccatttt
     5761 gaaacatttt tcagggtaat cattatgctg tccttctttt caagtagctg accaacaacg
     5821 acgcgctaag gctaataacg aaataaacaa atccggcaaa caggatcatc tcgacctgcg
     5881 taccatcacg ctcaccaatg gttgaggcgg tacggaagaa atcggccaga gataacacat
     5941 acaccagtga ggtatcctgg aacagtacga tgccctgagt gagcagcagc ggcaccatcg
     6001 cgcggaacgc ctgcggcaga ataatcagtt tcatcgactg ccagtgagtc attcccaacg
     6061 ccagcgcggc gctcgactga ccacgagaaa tactttgaat accagcacgg ataatctctg
     6121 aatagtaggc cgcttcaaac atcgaaaacg ccaccatcgc cgaaattaaa cggatatcat
     6181 tttttggcga taatcccagc acgttttgca gaaaacccgg cacgatcagg taaaaccaca
     6241 gcaaaaccat aactaaagga atcgagcgga atacgttaac gtaggctttg gcaaaccacg
     6301 ctacgggcgc aaagctggac aagcgcatca ccgccagcat ggtgccccac aaaataccaa
     6361 tcacgactgc cgtgacggtg attttcagcg tgatcaccag cccgtcgagc agatatggca
     6421 gggaaggaac aatggaactc cagtcaaact cgtacattat ttgcccccca tgttgccggg
     6481 caggcgaact ttacgttcaa ccagcgtcat caccagcatg ataaccgcgt taatcaatac
     6541 atacgccagc gtaatggcgg taaacgactc ccaggcgtga gccgagtagt cgagcaattt
     6601 acccgcctgc gctgccatat ccaccagacc gatagtcgag gcgatggcgg agtttttcac
     6661 caggttcatc atctcggagg tcatcggcgg gacgataacg cgataagcat taggcagcag
     6721 tacatagcgg taagcctgcg gtagcgtcag gcccatcgcc agcgcggcat tcttctgccc
     6781 acgcgggagc gactgaatag cggcgcgtac ctgttcgcaa acacgggcgg cggtaaacag
     6841 ccccaggcag agcatggatg aaaggaaaaa ctgaatattg ggatccagct cggccttaaa
     6901 ccacatgccg attttctccg gcaacagctc cgggatcacc agataccagg taaagaattg
     6961 cacaatcaac ggcacgttgc ggaacagttc gacatacaac gtacccagac cggagagaaa
     7021 acggtttgga acggtacgta aaatgccgaa aaatgaaccg acgaggaaag cgataatcca
     7081 ggcgcagatc gacaaagcga tcgtcacctg aaaaccactc cagatccaac caagataggt
     7141 ggtgttgccg aacggggctt gttgtaaaaa aataccccag ttccagtcta tagacataaa
     7201 tctactccag aaaaaagagg gtagcagcgt taactgctac cctcgaagat tgttacccag
     7261 cgtattgcgg ttttcaggcc cgatggggaa cgaccatcag gcgtatagtc tgtccgtgct
     7321 acgtaacaat cgagagggct ggaatttccg cccctggttc ttgtaattag ttcagtgcct
     7381 tgtcattcgg ttctttgaac agtgctttca tttcgtctga cagttcgaaa ttcatgttca
     7441 ggtttttcgg cggaattgga tttttgaacc atttatcaaa ccatttttcc gcttcaccgg
     7501 aggtctgcac ctgagcgatg gtgtcatcca tcagcttttt gaactgcgga tcatctttac
     7561 gcaacataca accgtaggcc tcctgagact gcggcttgcc gacgatttcc cagttgtctg
     7621 gtttcttcgc tttcgcacgt tcaccggcca gcagagcgtc atccatcata aaggcaacgg
     7681 cacgaccgct ttccagggtg cggaaagagt caccgtgatc tttggcgctg atgatgcgca
     7741 tattcatttt ttgctcttca ttcagtttgt tgagcaaaac ttcagaggta gtgccggaag
     7801 tgacgactac ggctttgcct ttcaggtcgg caaaatcttt gatatcgccg ccctttttgg
     7861 tcagcaggcg cgtaccgacc acgaaaatag tgtcagagaa agccgcctgt ttttggcgtt
     7921 cgacgttatt ggtggtagaa ccgcattcaa aatcgaaagt gccgttttgc agcagtggaa
     7981 tacggttttg tgaggtaatc ggaatcagtt ttacctgcaa gtccggtttg ttgagtttct
     8041 ttttcactgc ttcaacaatg gcgttggagt aatcctgcga ataacccacc actttttgct
     8101 gattgtcgta ataagagaaa ggtactgaag attcacggtg accgacgaca atcacaccgt
     8161 ttttggcgat tttgtccagc gtactgcctg ctgccggggc ggcgtcatct gcctgtgcca
     8221 gtcctgcgga aagcgccagg gcgaggattg ctgtggcagg tttacgtaat tgcatatcca
     8281 actcctttat cttctgcgtt aagaacgcat ggatacccgt tgtgagtgtt tgtgttgtta
     8341 tcatctgcaa ctttattgtg cagtattgtg cctgttaggt aacatttagt ttggctaaat
     8401 gtaaagatat tgctgtttta ttgtttgttt ttgcgagatg cgccgcacca ttccgaagca
     8461 aaattcttaa accgcacctt tttggtgcta ccgctgattt gtggtggtgc aacctggttg
     8521 catcgcggct gatttcattg cttacacagt aaataaagca acttacatgc caaaaaatac
     8581 ttaggagtga attaattacg attgtaattt caggctgaaa aagtcatatt tcggacagag
     8641 ttaacttttc ggtatgggac gtttgccggg atgataactg cacacgggga aaatgctttt
     8701 tacctgtctt ttcaaggaag aatcacctta tgtctgcatc ttcggttaag ccgttaaatg
     8761 ttcaattacc cgcaataacc cttatccttt ttgcgctctg tgttgggata ttttgttacc
     8821 tcgcacaatg gatgagttat gaagaagtcg atcaatccgc acttatccat ctcggtgcta
     8881 acgttgcttc tctcacgttg tcgggtgaat cctggcgctt attgagcagt gtctttctgc
     8941 acagcagtgt ttctcatttg ctgatgaata tgtttgcact cctggtggtg gggggagtgg
     9001 tggaacgtat tctggggaaa tggcgactcc tgattgtctg gctattctct ggcgtctttg
     9061 gtgggctaat cagcgcctgt tatgcgttac gcgaaagtga gcagatagtc atcagcgttg
     9121 gggcatccgg ggcaattatg ggaatagctg gcgctgcgat agcaacacag cttgcttcag
     9181 gtgcgggcac acaccataaa aaccagcggc gagtatttcc tctgttgggt atggtggcgc
     9241 tgacactgtt gtacggtgcc cggcaaacag gaatagataa cgcttgccac attggcggcc
     9301 tgattgcggg tggcgcgttg ggttggctga gcgcgcgttt atctgggcaa aaccgactcg
     9361 ttacggaagg cgggattatt gttgcgggca gtcttcttct gaccggggct atctggcttg
     9421 cgcagcagca gatggatgag tcagttttac aggtcaggca aagcctgcgt gaagcgtttt
     9481 atccgcagga gattgaacaa gagcgacgac aaaaaaaaca acagttagcg gaggaacgca
     9541 acgccctcag ggaaacatta tccgctccgg taagtcgtga acaggccagt ggtgatttac
     9601 tcgctgagat tgccgatatc catgatatgg cgatcagtcg ggatggtaat acgttgtatg
     9661 ccgcaattga aaacaccaac agcattgttg ttttcgacct cggacaaaag aaaatcctgc
     9721 atacctttac agcgcctata gcgaaagaaa agtcagtcaa acattgtggt ggctgtaaag
     9781 atcagggcgt cagatcgctg gcattaagcc cggatgaaaa gttgatttat gcgacgtcat
     9841 ttgaagcgaa tgcgttatcg gtcattaacg tggcgacagg ggagattatt cagtcgatta
     9901 ccaccggtgc acatcctgac agttttatcc tctcgcgtga tggcacaaaa gcctgggtga
     9961 tgaatcgcac cagtaatagt gtgtcagcga ttgatctggt gacttatcag catgtggcgg
    10021 atatcccgct ggagaaatac gacgggacgg ggacgagcgg taaaccaggc gcctgggtta
    10081 tggcactttc cccggatgaa agaacattac tggttccggg agcaggcaga ggtaacatcg
    10141 tgcggatcaa taccatcacg catcagaaag aagactttcc cgcaggtgat gcgcgtggaa
    10201 cgatatcggc gatgcgtttt cgacctgaaa acggcgaggt tatttttgcc gatagtcagg
    10261 ggatttcacg tataagcgta ggggatcaac aagccagcat tatgacgcaa tggtgtagca
    10321 ggagcgttta ttccgttgag ggtattagcc cggacggtca atatttagcg ttggtgtcat
    10381 atggcctgca aggttatgtc atcctgctca atattaatgc cgggcagatt attggcgttt
    10441 atcctgccag ctacgttaat caccttcgtt tttcagcgga tggtagaaaa atatttgtca
    10501 tggcgaagaa cgggttgatc caaatggaca ggacgcgctc gcttgatccg caggcaatta
    10561 ttcgtcatcc ccaatatggc aatgtggctt gtatccctga accgtaagaa aaaggggcat
    10621 gataaccatg ccccaaagct caatgagaaa gtacgttttg ctcatgcagg atacatcagg
    10681 cacatcaacg tttacgtgct cgcagactca tcagcacagc ggcaaaacca aacagcgccg
    10741 tcagcaccca cagcggccag ttgccggtac gtgcatacgg tgtgagtccg gtggtcggtg
    10801 tcacattagt ggtcaacacc tcgcgggtga actgcgggat catcgcctga atctcaccct
    10861 gcgggccaat caccgccgta atgccgttgt tggtgctgcg caacagtggg cgcgccagct
    10921 ccagcgcacg cattcgcgcc atctggaagt gctgccatgg accaatagac ttaccaaacc
    10981 acgcatcgtt ggagatagtc agcagatagt cggtatccgg gcggaagtta tcgcgcactt
    11041 gctcgccgag aatgatctcg tagcaaatag ccgcagtaag ctcaatacca tttactgaca
    11101 gcggcggctg gatatatggc ccacggctga acgacgacat cggcagatca aagaacggcg
    11161 ctaacggacg cagaatcgac tccagcggga caaactcgcc aaacggcacc agatggtttt
    11221 tgttatagcg atcggctgat tcgtagctgt acggtgcacc tttacccagc gtgatgatgg
    11281 tgttgtaggt atcgtagcgg ttctgcttat tgagacgcgc gtcgacaatc ccggttacca
    11341 gcgagctacc tttatcacgc aactcaccgt ccagtgcttt gaggaacggt tgctgattaa
    11401 tttccagatc ggttatcgcc gactccggcc agataatcaa cgatgatttg cccagcagcg
    11461 gtgccgttgc gttgtagtaa atcttcagcg tattaagaag ctggtctccg tcccatttca
    11521 gtgattgcgg aatatcgccc tgaaccatcg aaacctgaat ggttttctcc ggttgcgggg
    11581 taaaccattg gatgtaacgc agcgggaagg gaagggcaaa cagcacgacg gccaccacca
    11641 gcggacgcca gttgcgtttg actaacgcca gtgccagcag gccgctaacc atcatcagca
    11701 ggaagttaat ggcttccacg cccattattg gtgccagccc ttttaacggg ccatcaatct
    11761 ggctatagcc gaactgtaac cacgggaagc cagtcagcac ccaaccgcgc agaaactcgg
    11821 tcacttgcca gagagcaggg gcggcaatcg ccacgcgcag ccaggtggtt ttcggccaca
    11881 ggcgcgacag cacgccagca aacagtccgg tatacagcga caaatacgcc gccagcagca
    11941 ccaccaggaa gatgttaacc gggccaggca tcccgccaaa ggtcgcgatg ctgacataga
    12001 cccagttaat accgctgcca aagaggccaa atccccagca gaagccaata gcggcagact
    12061 ggagcgggcg gcggttaaag gtcagcgcct gaagccccat cagcgaaaca atcgccgcag
    12121 gccagacatc gtaaggagag aaggccagcg ttccgcaggc accgaataat aacgccagca
    12181 gcaggcgaat gcgctggcgt tcaattaatg aggcaaaagc catgtagtta tctatccagt
    12241 ttcggtttta ttcatccagc tttggctggg gtgagtcatc cgggattttg acatgaacct
    12301 gaataatacg ccgactatcg gccatcgcca ctttgaactg gtaaccgtcg atgtcgatag
    12361 tttcgccacg cgccggaaga tgcccaaacg cctgcatcac cagaccaccg atagtgtcga
    12421 cttcttcatc gctaaagtgg gtgccgaacg cttcgttgaa gtcttcaatg gaagccagtg
    12481 cgcgcacggt ccaggtatga cgactcagct gacggaagtc gatatcatct tcttcgtcat
    12541 actcgtcttc aatctcacca acaatcagtt ccaggatgtc ttcaatggtc accagaccgg
    12601 aaaccccacc gaattcgtca ataacgatcg ccatgtggta acgctgagag cgaaactctt
    12661 tcagcatccg gtctacgcgc ttactttcag gaacgacaac cgcctgacgt aacactttgt
    12721 ccatgctgaa ggcttcagca tcgctgcgca taaacggcag caagtctttc gccatcagaa
    12781 tcccttcaat gtgatctttg tcttcgctaa tcaccgggaa acgtgagtgg gcggactcga
    12841 tgatgacatc aaggcattcg tccagcgtct ggttgcgttt cagggtaatc atctgggagc
    12901 gggggatcat gatgtcgcga acgcgttggt ctgcgatgtc catcacccct tcgagcatat
    12961 cgcgcgtatc ttcgtcgata aggtcgttct gcccggaatc acggatcagc gccagcagtt
    13021 cgtcacggtt tttcggttca ccgtggaaaa gttggctgag taacagggag aaaaatccct
    13081 tcttgttgct tatcgtgtca ctactgtgtg aattgtcgtc gctcatggcg tcgtatgggt
    13141 tctcatgtta gttaatcaaa acgccgtcgt taatcaccaa cggcggggac gtctgccagt
    13201 caaatgcctg gcaaattatt ctttctcggc aatgtacgga tcctcatagc ccagagcaag
    13261 cataatctct gtttcgaggg cttccatttc ttctgcttcg tcatcttcga tgtgatcgta
    13321 acctaacaaa tgcagactgc cgtgcaccac catatgcgcc cagtgcgcct ccagtggttt
    13381 gccttgctcc tgagcttcct tctcaaccac ctgacggcag ataaccagat cgcccagtag
    13441 cgacatctcc atgccaggcg gcacttcaaa cgggaaggag agcacgttgg tcggcttatc
    13501 cttaccgcga taggtcagat tcagactgtg gctttcggcg gtatcgacca cgcgaatagt
    13561 cacttccgat tcttcctgaa actgcgggat caccgcattc agccatgttt gaaactggct
    13621 ctcttccggt aacccggaat tatcttcaca tgccagttgt aaatcgagga tcacctgact
    13681 catttttgtt cctgttcttc gcgcttgcgt tctgccgcca gcgccgcttt tcgtttttgt
    13741 tcggcttctt cccaggcttc ataggcgtta acgatacgcg ccaccacagg gtgacgaacc
    13801 acgtcttcgc tgtggaagaa gttaaagctg atctcttcga catcggccag cacttcgatg
    13861 gcgtgacgta agcctgattt agtattacgc ggcaggtcga tctgtgtgac gtcgccggtg
    13921 ataaccgctt ttgagttaaa accgatacgg gtcaggaaca tcttcatctg ttcgatggtg
    13981 gtgttctggc tctcatcgag aatgataaag gcgtcattca gcgtacgacc acgcatatag
    14041 gccagcggcg cgacttcaat aacgttgcgc tcaatcagtt tctcgacttt ctcaaagccc
    14101 agcatttcaa acagcgcgtc gtacagtggg cgcagatacg ggtctacttt ctggcttaaa
    14161 tcgccaggca ggaagcccag tttctcaccg gcttcgaccg ccggacgagt cagcagaata
    14221 cggcgaattt cctgacgctc cagggcatca actgctgcag cgactgccag gtaggtttta
    14281 cccgtacccg ccgggccaac gccgaaggta atgtcatggt cgagaatatt ggcgatgtac
    14341 tgcgcctggt ttggcgtgcg cggcttaatt acgccgcgtt tggttttgat attgaccgct
    14401 ttgccgtact ccggcacgct ctccgcactt tgctccagta ctcgcgcttc tttaatcgca
    14461 aggtggatct gttccggttc gatatcctga atctgaccgc gcatcggggc tgtatcgaca
    14521 tacaggctac gcagaatgtc tgccgcagcg gtgacgcaaa tcggacggcc ggtcagttta
    14581 aagtggttat cgcggcgatt gatctcgatg ccgagacggc gttcgagctg cttgatgttg
    14641 tcatcaaacg ggccgcacag gctcaacaga cgcgcgttgt ctgctggctc cagggtgatt
    14701 tcgcgagtgt ctatgttcaa accgttcctc ttatctgtat gccgccggaa gctgaacatt
    14761 caccggccta taaggaaatt attcacgcca caggaaaaag gcgcaagcat tgcgataaag
    14821 atggggataa agagagaaaa aacaaggccc accggaacgg caggcctgag aattacggtt
    14881 gataataacc cacgccaagg tcgttttctt tgcgagtacg ggcaatcact gattccggtg
    14941 tttctgccac gcgcagaccc atttcatctt cagtacgcac cactttaccg cgcagagagt
    15001 tcgggtagac gtcggtaatt tctacatcga cgaatttacc gatcatatcc ggcgtacctt
    15061 cgaagttgac cacgcggtta ttttccgtac gcccggaaag ctccatgatg ctcttacgcg
    15121 atgtaccttc taccagaata cgctgggtgg tgccgagcat ccggcggctc cacgccatcg
    15181 cttgctgatt aatgcgctct tgcagaatat acagacgttg cttcttctct tcttccggaa
    15241 catcatcaac catatcggcg gctggtgtac ccggacgtgc agagaagata aagctgtagc
    15301 tcatgtcgaa attgacgtcg gcaatcagct tcatcgtttt ctcgaagtct tcggtggttt
    15361 cgccagggaa gccaacaatg aagtcagaac tgatctgaat atccggacgc gccgcacgca
    15421 gtttacggat gatcgctttg tactccagcg ccgtgtgagt acggcccatc aggttcagaa
    15481 tgcgatcgga accgctttgt accggcagat gcaggaagct caccagctcc ggcgtgtcgc
    15541 gatacacttc gatgatatcg tcggtgaatt cgatcggatg gctggtggta aagcgaatac
    15601 gatcgatccc gtcgatcgca gcaaccagac gcaacagatc ggcaaacgat ccggtggtgc
    15661 cgtcgtagtt ctcaccacgc caggcattca cattctgacc gagcaggttg acttcacgca
    15721 cgccctgagc cgcaagctgg gcgatctcaa acagaatatc gtcggacgga cggcttacct
    15781 cttcaccacg ggtgtaaggc accacacagt aggtgcaata tttattgcag ccttccatga
    15841 tggagacaaa cgcggtcggc ccttcagcgc gcggttccgg tagacggtca aacttctcga
    15901 tttccgggaa gctgatatct acaaccgggc tgcggtcgcc gcgcacggag ttgatcatct
    15961 ccggcagacg gtgcagcgtt tgcggcccaa aaataatatc gacatagtgg gcgcgctggc
    16021 gaatgtgctc gccttcttgc gatgccacgc agccaccgac gccgataatc aggtctggat
    16081 tcttctcttt taacagtttc cagcgaccca actgatggaa gactttttcc tgagccttct
    16141 cgcggattga gcaggtgttc agcagcagca catccgcttc ttccgccacg tcggtcagtt
    16201 gatagccgtg ggtggcatcc agcagatcgg ccatcttcga tgaatcgtac tcgttcatct
    16261 gacagcccca ggttttaata tggagttttt tggtcatcga cttgctcttg cgaaatagta
    16321 gccaggaatg cagggcgcat agtgtaatgc tttgctgccg ttgtgaccag tatgagcgtt
    16381 atcagccctt gggggtaaaa atcctgtaaa cttaaagcat attgctaaca ggatgattga
    16441 ccatgacaaa tcaaccaacg gaaattgcca ttgtcggcgg aggaatggtc ggcggcgcac
    16501 tggcgctggg gctggcacag cacggatttt cggtaacggt gatcgagcac gcagaaccag
    16561 cgccgtttgt cgctgatagc caaccggacg tgcggatctc ggcgatcagc gcggcttcgg
    16621 tatcattgct taaagggtta ggggtctggg atgcagtaca ggctatgcgt tgccatcctt
    16681 accgcagact ggaaacgtgg gagtgggaaa cggcgcatgt ggtgtttgac gccgctgaac
    16741 ttaagctacc gctgcttggc tatatggtgg aaaacaccgt cctgcaacag gcgctgtggc
    16801 aggcgctgga agcgcatccg aaagtaacgt tacgtgtgcc aacctcgctg attgcattac
    16861 atcgagataa tgatcttcag gagctggaac tgaagggcgg tgaaacgatc cgcgcgaaac
    16921 tggtgattgg tgccgacggc gcaaattcgc aggtgcggca gatggcggga attggcgttc
    16981 atgcctggca gtatgcgcag tcgtgtatgt tgattagcgt acagtgtgag aacgatcccg
    17041 gcgacatcac ctggcagcaa tttaccccgg acggaccgcg tgcgtttctg ccgttgtttg
    17101 ataactgggc atcgctggtg tggtatgact ctccggcgcg tattcgccag ttgcagaata
    17161 tgaatatggc gcagctccag gcggaaatcg cgaagcattt cccgtcgcgt ctgggttacg
    17221 ttacaccgct tgccgctggt gcgtttccgc tgacgcgtcg ccatgcgttg cagtatgtgc
    17281 agccagggct tgcgctggtg ggcgatgccg cgcataccat ccatccgctg gcggggcagg
    17341 gggtgaatct tggttatcgt gatgtcgatg ccctgattga tgttctggtc aacgcccgca
    17401 gctacggcga agcgtgggcc agttatccga tcctcaagcg ttaccagatg cggcgcatgg
    17461 cggataactt cattatgcaa agtggtatgg atctgtttta tgccggattc agtaataatc
    17521 tgccgccgct gcgttttgtg cgtaatctcg ggttaatggc ggcagagcgt gctggcgtgt
    17581 tgaaacgtca ggcgctgaaa tatgcgttag ggttgtagcc ttacaacatt gccggatgcg
    17641 tgccaaccgt aggtcggata agacgcggca gcgtcgcatc cgacattgaa ggataagacg
    17701 tgtcaacatc gcattcgaca ttgaatgaac gcagaaaagc aaaaagctcg ccgaagcgag
    17761 cttttttaat gtggctgggg tacgaggatt cgaacctcgg aatgccggaa tcagaatccg
    17821 gtgccttacc gcttggcgat accccaactg ggcgcactta cgatgtaagc gtcttgaata
    17881 aattggctgg ggtacgagga ttcgaacctc ggaatgccgg aatcagaatc cggtgcctta
    17941 ccgcttggcg ataccccaac aaattggttt ttgaatttgc cgaacgtatt cgacacattc
    18001 agaatttggt ggctacgacg ggattcgaac ctgtgacccc atcattatga gtgatgtgct
    18061 ctaaccaact ga
//
LOCUS       DAZGXZ010000102        18006 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000102 DAZGXZ010000000
VERSION     DAZGXZ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.102"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..161
                     /locus_tag="ACHKJF_003669"
     CDS             <1..161
                     /locus_tag="ACHKJF_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HFP0950953.1"
                     /translation="NIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLS
                     IRIKVLMD"
     gene            193..1773
                     /locus_tag="ACHKJF_003670"
     CDS             193..1773
                     /locus_tag="ACHKJF_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFP0950954.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMGK
                     FIPVLAIPLIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKEKDDKAYGAFSGLIGF
                     MAMHLGTNFYLKQHDLLVAADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLFIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRRAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGLANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPLAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESSPVTLNAHEETAIANIPVHDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEAAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIC"
     gene            1773..2981
                     /locus_tag="ACHKJF_003671"
     CDS             1773..2981
                     /locus_tag="ACHKJF_003671"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFP0950955.1"
                     /translation="MDIFNTSISRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPE
                     AIIRTLKKRLEHPILGYSRWQHDDYLNSIVNWYHQQYKTEIQPEWITYSPSVMYSISK
                     AIEILTLNGDNILIFTPVYNAFFDVIKHNGRNMLTTSLMKENGRGYVINWQDFELKIK
                     SAKMVLLCNPHNPTGTVWSEDDLHRIAACCARHNIWLCSDEIHSDFVFNHSFTSALTI
                     KDDKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNVIATI
                     VAYNECEEWLKSLKVHIASNIQFTLQYLNENIPELIVWPAEGCYFSWVDCSALGYPFD
                     KFYRRLINEGRVGIMAGHVYGTEGESYLRLNLACSREKLYMGLARLASVIKNINQEKE
                     NEQYGSYPSY"
     gene            2950..3546
                     /locus_tag="ACHKJF_003672"
     CDS             2950..3546
                     /locus_tag="ACHKJF_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFP0950956.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
     gene            3618..4565
                     /locus_tag="ACHKJF_003673"
     CDS             3618..4565
                     /locus_tag="ACHKJF_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0950957.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(4644..4720)
                     /locus_tag="ACHKJF_003674"
     tRNA            complement(4644..4720)
                     /locus_tag="ACHKJF_003674"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4684..4686),aa:Met,seq:cat)
     gene            4929..6026
                     /gene="mltA"
                     /locus_tag="ACHKJF_003675"
     CDS             4929..6026
                     /gene="mltA"
                     /locus_tag="ACHKJF_003675"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP0950958.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            6103..6909
                     /gene="tcdA"
                     /locus_tag="ACHKJF_003676"
     CDS             6103..6909
                     /gene="tcdA"
                     /locus_tag="ACHKJF_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP0950959.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(6960..7403)
                     /gene="csdE"
                     /locus_tag="ACHKJF_003677"
     CDS             complement(6960..7403)
                     /gene="csdE"
                     /locus_tag="ACHKJF_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP0950960.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(7403..8620)
                     /gene="csdA"
                     /locus_tag="ACHKJF_003678"
     CDS             complement(7403..8620)
                     /gene="csdA"
                     /locus_tag="ACHKJF_003678"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP0950961.1"
                     /translation="MNVFNPAQFRAQFRAQFPALQDAGVYLDSAATALKPEAVVEATQ
                     QFYSLSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVA
                     QCYARPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPEL
                     ITPRSRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDID
                     FYAFSGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAG
                     TPNVAGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLA
                     FDFAGVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDAL
                     VNAVDRALELLVD"
     gene            8812..9039
                     /gene="ygdI"
                     /locus_tag="ACHKJF_003679"
     CDS             8812..9039
                     /gene="ygdI"
                     /locus_tag="ACHKJF_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP0950962.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9390..10307
                     /gene="gcvA"
                     /locus_tag="ACHKJF_003680"
     CDS             9390..10307
                     /gene="gcvA"
                     /locus_tag="ACHKJF_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP0950963.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            10326..10721
                     /gene="ygdD"
                     /locus_tag="ACHKJF_003681"
     CDS             10326..10721
                     /gene="ygdD"
                     /locus_tag="ACHKJF_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP0950964.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            10714..11814
                     /gene="rlmM"
                     /locus_tag="ACHKJF_003682"
     CDS             10714..11814
                     /gene="rlmM"
                     /locus_tag="ACHKJF_003682"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP0950965.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(11858..12589)
                     /gene="fucR"
                     /locus_tag="ACHKJF_003683"
     CDS             complement(11858..12589)
                     /gene="fucR"
                     /locus_tag="ACHKJF_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP0950966.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12647..13069)
                     /gene="fucU"
                     /locus_tag="ACHKJF_003684"
     CDS             complement(12647..13069)
                     /gene="fucU"
                     /locus_tag="ACHKJF_003684"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP0950967.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(13071..14489)
                     /gene="fucK"
                     /locus_tag="ACHKJF_003685"
     CDS             complement(13071..14489)
                     /gene="fucK"
                     /locus_tag="ACHKJF_003685"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP0950968.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(14598..16373)
                     /gene="fucI"
                     /locus_tag="ACHKJF_003686"
     CDS             complement(14598..16373)
                     /gene="fucI"
                     /locus_tag="ACHKJF_003686"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP0950969.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(16406..17722)
                     /gene="fucP"
                     /locus_tag="ACHKJF_003687"
     CDS             complement(16406..17722)
                     /gene="fucP"
                     /locus_tag="ACHKJF_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0950970.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
BASE COUNT         4534 a         4165 c         4580 g         4727 t
ORIGIN      
        1 aaaatattgg tgatatctat gagcagtttc ttgaaaaaga ttttcagtta caacgtgcca
       61 ttcatgaaat tgaaagatat ctgtacgcaa cttttaaata tgatttgatt ctggaagaga
      121 aactatatct ttctattcgc atcaaagtat taatggacta attatacata acattttcat
      181 gagataacca gtatgaaaca aaagaaagcc tggagttttt ttcagagcct ggggaaggca
      241 tttatgtatc ccatcgctct gttaagtgta tgtggtatga tgttagggct gggaagtggt
      301 ttagccagcg atgatatggg aaaattcatt ccagtgttgg ctattccgtt aattaaaacc
      361 atacttgatt ttattgttag ccttggtttg tttgccttcg ttaatttacc tgtattgttt
      421 gcgatagcta tacctttggg attattaaaa gagaaagatg ataaagccta tggcgctttt
      481 tctggcttaa ttggctttat ggcgatgcat cttggaacga acttttatct taaacagcac
      541 gacttattgg tagctgctga ccaaatgtcg acacatgggc aaaccatcat actcgggatc
      601 cagtcctaca ataccagcgt gttgggtggg attgtggcag ggttattagt cgccagcatg
      661 tataaaaaga tcgttaactt acgtattcct gaatcattag ggttttatag tggcccacgc
      721 cttgtgccta tcattacact gattgtgatg agtggatttg gtctgttcat tccttttatc
      781 tggccgccat ttttcaatct tttcatgctc attggtcact ggatttcaac ttccggtcct
      841 gttggttatt tcttctatgc tgttgccgaa cgcgtgacga taccttttgg cttaaatcac
      901 ttggtgacat cagttttccg ctttacccca atcggcggat cggcagtgat tggcggtgaa
      961 gagtattacg gcaccctgaa catgtttatg gcatacgtca aagagaatgc ggtcattccg
     1021 ctggatttgg cggggaaaat ggagcagggc aaactgatga ttcagtatgg tctggctgga
     1081 gccgcgctgg cgatgtatcg cactgctcat gctcaaaaca gaagggctat caaagcattg
     1141 ctgatttccg gtgtgctcac ggtaattatt ggcggcgtca gcgagccgat tgaatttctg
     1201 ttcttatttg tcagcccgct gttgtttgtc ttccatgcct ttatgaatgg attggctaac
     1261 atggttctgc catatatggg cgtgaagatg ggctttactg gcgatctgat tcaatttatt
     1321 agctttggtg tcttacgtgg cacaagaacg ggatggccat tagcggtatg tgttgaagtg
     1381 gcttatttct tcatttatta ctttgtgttc cgttggacca ttctcaaatt taacctgatg
     1441 actgtaggcc gtgaagagtc cagtcctgtc acgctgaacg ctcacgaaga aactgcaata
     1501 gctaacatcc cagttcatga taaatcagag ttgcaggcag cggagcaaat ggttaaggct
     1561 ctgggtggta aagaaaatat taaatcactg gataattgtg taactcgttt gcgtttaact
     1621 atcgcggata tgggattgat agatgaggct gcaataaaaa gggccggagg aattgcggtt
     1681 gtgaaacttg atcaaaatac cttacaagtc attatcggta caaaagtcat cgccctacgt
     1741 cgggatatgg ataattacat ggggatatgt tgatggatat ttttaatacc tcaatttctc
     1801 gtaaaggaac atattgcaca caatgggact tttgtgaaga cagatttggt gtaaaagatg
     1861 tattaccatt ctctatttca gatatggatc ttccgatacc ggaagctatc atcagaacat
     1921 taaaaaaacg tctggaacac ccgattttag ggtacagtcg ttggcagcat gatgactatc
     1981 tgaattcgat agtcaattgg tatcatcaac aatataaaac agagattcaa ccagaatgga
     2041 tcacctatag cccaagcgtg atgtattcaa tatctaaggc aatagaaata ttgacattga
     2101 atggagataa tattctcatt ttcacgcctg tttataacgc attttttgat gtgattaagc
     2161 ataatggtcg aaacatgcta acaacctctt tgatgaaaga aaatgggaga ggctacgtta
     2221 tcaactggca agactttgag cttaaaatta aaagcgcgaa gatggtgtta ttatgtaatc
     2281 ctcataaccc gacaggaacg gtctggtcgg aagacgatct tcacagaatc gcagcatgct
     2341 gtgcaaggca taatatttgg ttatgttcag atgaaataca tagtgatttt gtttttaatc
     2401 atagtttcac gtcagcgcta acaataaaag atgacaaagt ggtggtgttt aattcgattt
     2461 cgaaaacatt taatgttcct gcgctaaccg gatcatatat gatttcaact gacgataatt
     2521 ttaatcataa gttcagaacg atatcgaggt atcgcgattt cgtaaactcc ccatcagtac
     2581 tgaatgtaat tgcgaccatt gttgcctata atgaatgtga agaatggtta aagtcgttaa
     2641 aggtacatat tgcctctaac attcaattta ccttgcagta tttaaatgaa aatattccgg
     2701 aattgatcgt atggccagct gagggctgct atttttcgtg ggttgattgc tcggcccttg
     2761 gttatccctt tgataaattt taccgtcgat taattaacga agggagagta ggtattatgg
     2821 cggggcatgt ttatggtact gaaggagaga gctatctgcg tcttaatctg gcctgcagtc
     2881 gagaaaaatt atatatgggg cttgcgcgcc ttgcgagtgt aattaaaaat attaatcagg
     2941 aaaaagaaaa tgaacaatac ggatcttatc catcttatta aacgttttat gcgtaacgaa
     3001 cataaagcag tagaagaggt tattgattcc cccctttcgg aagtcgcgaa tttaattaaa
     3061 atattacaat cttgccaggg aaaagttgtt tttattggtg ttgggaaatc tggcattatt
     3121 gccagaaaac ttgctgcaac atttgccagc accggaactc cctcgttttt tgttcacggt
     3181 acggaagcgg tacacggcga ccttgggatg gtggcgaaag acgatgttgt tattcttatt
     3241 tcaaacagtg gtgagacggc ggaaatcctg gcgacactgc ctagtttgaa aaaaatgggt
     3301 aattatttga tctcttttac ccgcagtcat cactcatcgc ttgcgataag ttgtgattta
     3361 tctgttgaaa tccccgtcaa aagtgaggcg gataatttag gtttagcacc gtcatgctcg
     3421 tctactgttg ttctggttgt tggtgatgcg gtggcgcttg cactctctga attgaaaaaa
     3481 tttactcgtg ctgatttcgg cttatatcat ccaggaggtg cactcggcat taaagcaaat
     3541 tcataatttc aaagtataaa tacagtaacc ggtcacgcca tcttttgggg tggcgttcat
     3601 tgaagtgagt gtcagctatg aaaaatgttc tggtcaccgt ccccagcttt tcagcacgct
     3661 gtgtttccgc cagcaagttg ctaagagaga ataattttaa tcttattatt aagaataatg
     3721 tggagcatct gcttaaatca gaatccactg cattacgaga atctatttgc gcggtcatcg
     3781 ccggaaaaga ttgttatcag gcggacacgc tatcattgct gcctggtgtg aaaataatat
     3841 cgagattcgg gacggggata gataatattg atcttcgtgc agcacaacaa tccggtattg
     3901 tagtcaataa tgcggttggg attaattcaa atgctgtcgc ggaatttatc attggactta
     3961 tcttcgcgag catgagaaat attccaggca gctatcatgc gatgcaaaat ggctactggg
     4021 gtgagtcgca tggctatgaa ttacaaggca aacggattgg catggttggc tacggcaata
     4081 taggtaaaac cctggcaaaa aggttatccg gttttgatgt tgagctttta gcttatgaca
     4141 aacaacccga ttatcaggtt gctgacaaag ttggagttca gtttgtatca attgaagata
     4201 tctttatgca gtcgcatgtc atcattgttc ttttgccttt ttctcctgag ctggagaact
     4261 tcattagtca taaatatttg tcgatgatgc gcaatggtgc tctaatcatc aatgccgcaa
     4321 ggggaaagtt gctggatgag ggcgctttac tacaggtgat tgaagaacga aatgtgtttg
     4381 cggcgctgga tgtgtttagc tctgaaccgt tggcgcaatt tagcccactg ctacatgcca
     4441 aaaatatcat tacaacgccg catattgcgg ccgccaccgt tgagtcatac cagcaaaccg
     4501 gtacacacgt tgcccagtcg ataattgatt acttcgcagg aagggagata aaaaacgtgt
     4561 tgtgagttca gcgaacgaag tttttaaata caaaaaaacc gcaatttgtg gaacacattg
     4621 cggtttatca gcattctaag tattggttgc gggggccgga tttgaaccga cgaccttcgg
     4681 gttatgagcc cgacgagcta ccaggctgct ccaccccgcg tccgtggatg cgcactatac
     4741 tccgctcgcg ttttcatgca accttttttt taccccgatc acggatttag catgattttg
     4801 aacaaaaaca aaccactaaa gcctattttt tgtgcaaaat ttgcgtcagg gagaggctgc
     4861 tcatttcatt aaacgcggcg gtttgttatc ttcgttgcgc cttatttttt aacctgaaga
     4921 agagaacaat gaaaggacgt tgggtaaagt accttcttat gggcacggtt gtggcaatgc
     4981 ttgccgcctg ctcttccaaa ccaaccgatc gcggacagca atataaagac gggaaattta
     5041 cccagccttt ctctctggtg aaccagccag atgccgttgg cgcgccgatt aacgccggtg
     5101 attttgccga gcaaattaac catatccgta attcgtcacc gcgtctgtat ggcaaccaga
     5161 gtaatgttta taacgcggtg caagagtggc tgcgcgcagg cggtgatacc cgcaatatgc
     5221 gccagttcgg cattgatgcc tggcagatgg aaggtgccga caactatggt aacgtgcagt
     5281 ttacaggcta ttacacgccg gtaattcagg cgcgccatac ccgccagggc gagttccagt
     5341 atcctattta ccgtatgccg ccaaaacgtg gtcgtctgcc gtctcgtgcg gagatctacg
     5401 caggggcgct gagtgataaa tatattctcg cttacagcaa ctccctgatg gataacttca
     5461 ttatggatgt gcagggtagc gggtatatcg actttggtga tggcagtccg cttaactttt
     5521 tcagctatgc agggaaaaac ggtcatgcct atcgcagcat tggtaaggtg ctgatcgacc
     5581 gtggcgaagt gaaaaaagaa gatatgtcga tgcaggcgat tcgtcattgg ggcgaaacac
     5641 acagtgaagc cgaggttcgc gagctgctgg aacagaaccc gtctttcgtt ttctttaaac
     5701 cgcaatcttt tgcaccggtg aaaggggcaa gtgcggtgcc gctggttggc cgtgcttctg
     5761 ttgcctccga tcgttcaatt attccgccag gtactacctt gctggcagaa gtgccgctgc
     5821 tggataataa cggcaaattt aacggtcagt acgaattgcg tctgatggtg gcgctggatg
     5881 tcggtggcgc aatcaaaggc caacacttcg atatctatca agggatcggg ccggaagccg
     5941 gacatcgcgc aggttggtac aaccactatg gacgtgtctg ggtgctgaaa accgccccgg
     6001 gcgcaggtaa cgtctttagc ggctgatgtg ttattctgta taccgttttt acggatacgc
     6061 cagggtgagg gataacctca ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt
     6121 gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcgttgcaa
     6181 ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg
     6241 gaagcgctgg cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc
     6301 gtcaccaata ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg
     6361 gaagtcatgg cggagcgtat tcgccagatt aacccggagt gccgtgtaac ggtggtggat
     6421 gatttcgtga cgccggataa cgtagcgcag tatatgagcg tgggttattc gtacgtgatt
     6481 gatgccattg atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa
     6541 atcccgctgg tcacgaccgg tggcgcgggt gggcagattg acccgacgca gattcaggtt
     6601 accgatctgg cgaaaacgat tcaggatccg ctggcggcga agttgcgcga gcgcctgaaa
     6661 agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg gcgtcgattg cgtgttttct
     6721 actgaagcgc tggtgtaccc gcagtcagac ggtactgtgt gtgcgatgaa agccacggca
     6781 gaagggccga agcggatgga ttgtgcatct ggatttggcg cggcaacgat ggtgaccgcc
     6841 acctttgggt ttgttgcggt ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt
     6901 cagggttaaa caatcaggtc ggatgcgacg cgaacgtctt atccgacctg gttctgtgct
     6961 taaacctgct tcgccgcagc gataatcgcc tcgcttaacg cattcaaccc ctggctgcgt
     7021 gaggcgctaa gctgtgcacg caatcccagc tcatcaaaca atgccagcgg cgactgtgcc
     7081 tgcaattcgg cgacggtttt cccctcaacg gcagtcaaca acaccgccaa caggccgcgc
     7141 acaatgcgtc cttcgctgtc gccaaagaag tgcattttac cgttttcagt cacggtatat
     7201 cccagccaga cgcggttttc gcatccggca atctctttag cctgcgcttt taactcgtct
     7261 ggcaaagccg gaagctgttt ccccagcatg atcaactggc gatatttatc ttcccattgc
     7321 gacaacggtg cgaaggtatt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt
     7381 ccggcgaatt gcgggtttgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat
     7441 tcaccagcgc atccacatca ctctttgtat tatatggcgc aaaagaggcg cgcagtgtgc
     7501 cggttacgcc taattctgcc agtagcggct gagcgcaatg ctgcccggcc cgcagggcaa
     7561 taccgtactc cgccagcagc gtcaccatat cgctatgatg aacgccagca aaatcaaagg
     7621 ccagcaggct ggaatcctgg cagcggaatg aacgaaagcc gggacgtttc gccagcgcat
     7681 cttccgccag cgttgctaag ctacggctcc agctttcggc ctggttgata tcgtaatctg
     7741 ccagccattc cagcgccgcg cttaatccta tgacaccagc gacatttggc gttccagctt
     7801 ccagtttcca cggcgcagat tgagtcgtga agccgtcaaa actcacttcg tgaaccattt
     7861 tgccgccgcc cagccagggc gacatcgcct ccagcagttc tgatttacca tacagcacgc
     7921 cgatacctgt cgggccatac agtttgtgac ctgaaaaagc atagaaatca atatccagtt
     7981 gctgaacatc cgcggggaaa tgcactgccc cctgagcacc atcaaccatc accaccatcc
     8041 cggctgaatg agcaaaggta atcgctcgcg ccagatccgg gcaaccgcca gtaacgttcg
     8101 acatctgacc caacgccaga atccgactac ggggagtaat cagttctggc aacaaatcga
     8161 catccggcag tcgctgcgca ttaagcggca atttcaccac tttggctcca gtttgttggg
     8221 cgaccatcag ccaggggacg aggttggcgt ggtgttctgc cacgctgaca ataatctcat
     8281 cgcccggttg cagacgcgga cgcgcatagc attgtgccac catgttgatg gattcagtgg
     8341 tgccgcgcgt ccagacgata gttttatcat ccggtgcatt cagtaattgc gccactttct
     8401 ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga
     8461 cgtttccggc gctcagactg taaaactgtt gggtggcttc aaccacggct tcaggtttaa
     8521 gcgcggtcgc ggcgctgtcg agatagacgc ccgcatcctg tagtgcggga aactgggcgc
     8581 gaaactgggc gcgaaactgc gcgggattaa aaacgttcat ggtactcctc ggcttgatgc
     8641 accgatcgtg acgcaaaatt cactttgata caaggagagt gctaaccatc agaattatct
     8701 ggatatcctg gcgaagccgc gcaagtaaca tatgctgaat agtgttaggc gttaatttta
     8761 gcctgttaat caatactatt aagatttatt aatcgcttta aggagaagat gatgaaaaag
     8821 actgccgcaa ttatttctgc ctgtatgctg acttttgccc tgagcgcctg ttccggttcg
     8881 aactatgtga tgcacaccaa tgacggacgt accatcgtct ctgacggcaa accacagact
     8941 gataacgata ccggtatgat ttcgtataaa gacgctaatg gcaacaaaca gcagatcaac
     9001 cgtactgacg tgaaagagat ggtcgaactg gatcagtaat tcgcgatcgc aaggtaaaaa
     9061 aaagcaccgc aattaggcgg tgctacatta atcactatgg acagacaggg taaatgtaca
     9121 ggaagtgaaa aaaggtagct ttgctaccat ggtctgaatc gcagaccaat tgcaaacaca
     9181 acaacacaac atcacaaccg taagccaaaa gttcaccaga acacgcattc cgataaaact
     9241 tttcgttccg gctcaggaag tgccgccact ataggtattt gctggtagaa gctcaacgga
     9301 caatttataa tggctcagat taaaaaaact aataggttac acagtgtgat ctaattgtta
     9361 aattcattta acatcaaagt ttaaaagcca tgtctaaacg attaccaccg ctaaatgcct
     9421 tacgagtttt tgatgccgca gcacgccatt taagtttcac tcgcgcagca gaagagcttt
     9481 ttgtgaccca ggccgcagta agtcatcaaa tcaagtctct tgaggatttt ttggggctaa
     9541 aactgttccg ccgccgtaat cgttcactcc tgctgaccga ggaagggcaa agctatttcc
     9601 tcgatatcaa agagatattt tcgcaattaa ccgaagcgac gcgtaaactc caggcccgta
     9661 gcgccaaggg ggcgttgacg gtcagtttac tccccagttt cgccattcat tggttggttc
     9721 cgcgactttc cagctttaat tcagcttatc cgggaattga cgttcgaatc caggcggttg
     9781 atcgtcagga agataagctg gcggatgatg ttgatgtggc gatattttat ggtcggggca
     9841 actggccggg gctacgggtg gaaaaactgt acgccgaata tttattgccg gtgtgttcgc
     9901 cgctactgct gacaggcgaa aaacccttga agaccccgga agatctggct aaacatacgt
     9961 tattacatga tgcgtcacgc cgtgactggc agacatatac ccgacagttg gggttaaatc
    10021 atatcaacgt tcagcaaggg ccaattttta gtcatagcgc catggtgctg caagcggcta
    10081 ttcacgggca gggagtggcg ttggcaaata acgtgatggc gcaatctgaa atcgaggccg
    10141 gacgtcttgt ttgcccgttt aatgatgttc tggtcagtaa aaacgctttt tatctggttt
    10201 gtcatgacag ccaggcagaa ctgggtaaaa tagccgcctt tcgccaatgg atcctggcga
    10261 aagccgctgc tgaacaagaa aaattccgct ttcgttatga acaataattt acgtagggta
    10321 cgaccatgac cagccgtttc atgctgattt tcgccgccat tagcggcttc atttttgtgg
    10381 ctctgggcgc ttttggcgcg catgtgttaa gtaaaaccat gggggccgtt gagatgggct
    10441 ggatccagac cggcctcgaa taccaggcgt ttcatacgct ggcgatctta ggtctggcgg
    10501 tggcaatgca gcgtcgcatc agtatctggt tttactggag tagcgttttc ctcgcgttag
    10561 gcacggtgtt gttcagcggc agcctttatt gcctggcgct gtcccatctg cgtttgtggg
    10621 cgtttgtcac tccggttggc ggcgtgagct tcctcgcggg ctgggcgtta atgttagttg
    10681 gtgctatccg tttaaagcgc aagggcgtaa gtcatgaata aggttgtatt gctgtgccgt
    10741 ccgggctttg aaaaagagtg cgccgcagaa attaccgata aagccggcca gcgggaaatt
    10801 ttcggttttg cccgcgtgaa agagaatgcg ggttatgtca tttatgaatg ttatcaacct
    10861 gatgatggcg ataagttaat ccgtgagctg ccgttcagtt cattaatttt tgcccgccag
    10921 tggtttgtgg tgggggaact cctgcagcat ttgccgccag aagatcgtat tacccccatt
    10981 gtcggcatgt tacagggcgt agtagagaag ggcggtgaac tgcgtgttga agttgccgat
    11041 accaacgaaa gcaaagagtt actgaaattc tgccgtaaat ttactgttcc gctacgcgct
    11101 gccttgcgcg atgccggggt gctggcgaac tatgaaacgc cgaagcgtcc ggttgtgcat
    11161 gtattcttta ttgcaccagg ctgctgctat accggttact catacagcaa caataattcg
    11221 ccgttctata tgggcattcc gcgcctgaaa tttccggcag atgcgccgag tcgttccacg
    11281 ctcaaactgg aagaggcatt tcatgtgttt attcctgcgg atgagtggga tgaacgcctg
    11341 gcgaacggga tgtgggcggt ggatttaggc gcttgccctg gcggctggac ctaccaactg
    11401 gtgaagcgca acatgtgggt ttattccgtc gacaacggcc cgatggcgca aagtctgatg
    11461 gataccggac aggtgacgtg gctgcgggaa gacggtttca aattccgtcc gacgcgcagc
    11521 aatatctcct ggatggtatg cgatatggtt gaaaaaccgg cgaaagttgc ggcattgatg
    11581 gcgcagtggc tggttaatgg ctggtgccgt gaaaccattt tcaacctcaa actgccgatg
    11641 aaaaaacgct acgaagaagt gtcacacaat ctggcgtata ttcaggcaca gcttgatgaa
    11701 catggcataa atgctcagat tcaggcgcgg cagttgtatc acgatcgcga agaagtgacg
    11761 gtgcacgtcc gccgcatctg ggctgcggtg ggtggtcgtc gcgacgagcg ataacaggca
    11821 aggccggggc gtcgtctccg gcctgctacc ctttccgtca ggctgttacc aaagaagttg
    11881 caacctggcg ctcatcagaa ataatgtgcg ttacctcatc cagatgcccg atgcgggctt
    11941 cccctgaacg attaaattta cttttatcaa tcaataacaa cgattgcgcg gcacgtttta
    12001 atagcatcga tttgtaatca gcgttgatcg cattggagtc ccacagtgcg ccgctgctat
    12061 cgatcccttc acaagaaaaa ataaacagat cgatttccag cgatttaagt tgggaaatca
    12121 gcgagggatt gacgtaacag ccatatttgc gctcaagtgt gccgccggaa ctgatcagtt
    12181 gaatgcgttc gcgtttaccg agttcatggc aaatcggatg gctattggtg aagacctgaa
    12241 tgttgatgtc aggcaactgg cgtgccagat accagcaagt tgaactggcg tctaaggcta
    12301 tcaccatccc ttcttcaatc cacgcgagcg cctcgcgcgc gatatctgct ttatgcgcat
    12361 aatggctttt cagcctgatg tgaaagggat cgccactgtc ttgattttga cggtggatat
    12421 atttagcgcg tccatgattg cgcagaattt taccctgcgt ctgtaattca ttgagatcgc
    12481 gacgaatggt ttctttactg acctttagct gttcagagag agcttccgtg gtcaggctgg
    12541 tatggttcag cagcaggtcg actatcgctt gctggcgtgc cgctttcata tttttcaccc
    12601 catcattcga taaccgcccg cgtcatgcgg gcgtaccggt atgagattac ggtgttaccc
    12661 ctttttttaa aagaatattc ccgtacttcg ctcgttcgcc tgtgataacg atcgcaaagg
    12721 ctttttgcgc ccgttcataa aacgcaaaac gattgatgcg gatgatgtca ggacacgggg
    12781 cttgtagtga aagcgcatta cggtaacgtc gttctacttc aggatcgaga gtgtcacctt
    12841 ctaccgccgc catcatcacc agcggcggtg cataactgtc cagttcaaat aacgggataa
    12901 tcgcctggag caagtcgctc accaacaggc catcagcgcg gatcacctgc ggtcccatcg
    12961 aatgggcggg aaagtgagca tcggaaaaaa taatttcatc tccatgtccc atctctgcca
    13021 gcactttcaa tagttcggga gaaattaacg gcgaaattgt tttcagcatt tcacacttcc
    13081 tctataaatt caggttcagt ttgcgggtag aaataacggt actgataatg aatctgtgcg
    13141 cgggcttctt ccgggctgtt aaattcccct acgccatacc aaccgaacag cgcagctcct
    13201 gcgacggtcg tttcggcgtc gtcgagaact tttaccggaa tatcaagcat attggcttta
    13261 atctgattcc acaatgtgtt gcgacttcct ccaccgacta acaataattc agaggcctta
    13321 aagtgcccga ttttttccag catctgtaga ttgcgctgta attgcgcagt taacccttcc
    13381 agcgccgcgc gatagaaatg cccccgcgtg gtattaagcg tcactccttg ccagccagcg
    13441 ttctgacacg acaataaatc acactgcatt tttacgccat ccgcgccagg cgcgatcaga
    13501 cgagcttctt caatcaacat ttgccagggt gtttcagccg tccagaacag ttttctcacc
    13561 cattccagca cgccggatgc cagccattgc atacctgggt tatacaaccc tgcctggcta
    13621 tccagttcgc aggtggaacc ggcgtactga cttaacagcg aagtatcaac ctgggcgcta
    13681 cggaccatta aaatttccca tgtaccggaa gagagcacgg gttcattttg gtcggcacca
    13741 gcgccaaaaa gggcgaactg ggtatcgtga cctgcggaaa tcaccggtat gccaacgggt
    13801 aagccgagca ttgctgcggc gctgttctgt agcgtaccaa tctgttcacc cgcttccacc
    13861 agacgaggga agagtcggcg tggaataccg gtggcttgta aaatttgcgg actgaaatcg
    13921 cgttgctgga tatccagcat ctggctggtt ccggccatcg tgatatcagt agtgaattcg
    13981 ccggttaaac ggtggttaat cagcgacgaa ataaagagcc aggcgtgcgc gcgttccagc
    14041 agttgtggat gattttcttt caaccacacc aacttatata acgtattgaa actaaaggct
    14101 ccgacgccag aaatagcctg caaccgctgt gcggagatca actgttcaat attgtccata
    14161 accgctgctg ttcgcggaca tttccagcta atgatcggat agagcagatt gccttgctta
    14221 tctaccagag cgccatccac accaaaggtg gtgacggcga taccgcggat gtggcattca
    14281 gtcagttcac tattgatttg ccgacagcaa tcagcaaagc gttgcaaaat ggcgtctaaa
    14341 gaccactggt gccaggtgtt gttttccatc gcgatatcgc tggcattagg cgttgaggcg
    14401 cgggcaacaa tttttccctg ccgattaacc gcgatggccc tgacattggt cgcgccacag
    14461 tcgagtacca ggataacttc ttgtttcata atcgctcctg caatatagcc ggataacatt
    14521 gcttatccgg ctaaccactc ttgcttgttc aggcccggta gccggagcga ccgggcatca
    14581 catcagggag taatgtatta acgcttgtac aacgggccgt agttctggca agcgcggtaa
    14641 tcctgacctt caatatccat gccgtgcgcg gcccaggcag aaggacgata tactttggtc
    14701 tcttcaacgt tgtgcataca taccgggata cgcagcatgg aggcgagagt gataaagtct
    14761 gcgccaacgt ggccgatggt cagaaccccg tggttagcgc cccagttcgc cattaccgaa
    14821 tacacatccg taaacggccc tttaccggtg aggcgcggtg caaaccaggt ggttggccag
    14881 gttgagttgg tgcgtttgtt gaggatgtca tgcacatcct tcggcaattc cacgctccag
    14941 ccttccgcga tttgcagtac cggtcccagg cctttgatga tattgacacg ggtcatggtg
    15001 aacgggacgc cgccttcggt aaggaagcgg gaagagtaac cgccgccacg gaagtattcg
    15061 tggatcgccg ggcaccattc ggtagcggcg aggcaagcgt cagcctcttg ctgagagatt
    15121 tcccagtgcg gcttcatcgt cgggttacct tcgctgtcgc gttgtttaca ggaaccgtcc
    15181 agcgcagcag aacctgagtt gatcaaatgg atgatgccgt gttctgccag tccatcgagt
    15241 ttatgccccg ttacacgctc aattgcttct ggtgaccagt aggtacgcac atcggcaaat
    15301 acctgagcgg tgccggtgag ctggtgaccc attagcattg ccacgccgtt aagactgtcg
    15361 ttttcggtcg ccacgacaaa gggttcgcgc acgccattcc agtcaaatga actgttgagg
    15421 atcgcttcgg cggtgtcacc attgggatat tgatcggtcc agtgacgttg cccctggaag
    15481 cccgcagcga tggcgttgta gccaagtgat tcttccacgc gaccaatatc ggccagtttg
    15541 ctgttgcctt gcatcatgtc gcggatacac atcgccatca gtaaactttc gcgcagaact
    15601 gcgcggcttt gctcggcatt gcgttgatac tgtttgttat tttcatcttc gccatagcgg
    15661 aagtttttat cagcccaggt cagtgccatt tccagttcgg cttcgtcgta aatcttctga
    15721 tcgatacggc gacgcagttc ggtcatatcc accgcctgga ctttcattcc cagccaggat
    15781 tcaaagaagt tgtgatcaac aatagaaccg gcgataccca tcgaaacgcc gcccagcgac
    15841 agatagcttt tacctttcat gctggcgacg gccaaaccgg cgcgggcaaa gcgcagcagt
    15901 ttttcttcaa catcggcagg aatcgatgtg tcatcggcat cctgaacgtc atgaccgtaa
    15961 atggagaatg ccgggatgcc tttctggctg tgagctgcca gagccgctgc caggtaaaca
    16021 gcgccgggac gttcagtgcc gttaaagccc caaatggctt tcgggcgggt tggatccatg
    16081 tcgatggttt cactgccata gcaccagcaa ggcgttaccg taatggtgag gcctacattc
    16141 tggctgctga atttttcttc gcaagcagcg gcttcagcca tacccgcgat acaggtatcg
    16201 gaaatgacac actcgacggc agctccgcag gcatggcgca gtttctcggt cagcagtgcg
    16261 gccgtagctt tcgccatatt cattgtttgt tcttcaagcg actcacgaac acccatgcga
    16321 cgaccgtcaa taaccgggcg gataccaatt ttcggtaagc tgattttttt cattacagat
    16381 tcctcacttt attcggaaaa tatgttcagt tagttgccgt ttgagaacgg aaacgggcaa
    16441 agataaagat gaccgcgaag cagagtgcgg ggatcagttc agcagtgggg atgttgcccg
    16501 ccgcgtcact gacaaaaccc atgaccggag tgacaatacc gccgccaatg atggtcataa
    16561 cgatgaagga cgaaccgtat ttggtgtcct ggccgagatt cttaatgccc agcgagaaga
    16621 ttgttgggta ctgaatcgac ataaaggcgc tgcataaagt cagggctatt aagcccacat
    16681 gaccgcctgc gaaggctgag atcaggcaca gtgccatagc gattaatgcg taggcggcca
    16741 ggactttgtg tggtgcgaag cgactgatga gccaggtacc ggtgaaacga ccaataaaga
    16801 agcacaccat ggttccggtt aaatagttag cggcaaagcc tgcagtcata cctggaattt
    16861 cttctacagc gtagcgaatc aaatagctcc agcaggccgt ttgtgcgccg acatagcaga
    16921 attgcgctaa taccgcccag cgccagtgac gaatacgcgc cagacgagaa agcgatgcgg
    16981 agaacgatcc ttgtttggcg tcactgtgat tatcactctg caatgccggg aatttcgtca
    17041 gcatgatcag cagggcgacc agtaacacga tagccacgat gatcatataa ggtgtctgta
    17101 ccgataatac caggctgtgt ttatacgcac tcaattgctc tggagacatt ttatcgagaa
    17161 cgtcttgcga ttgatgtggc acgttagaca aaataagact ttgcccaaag acaaccgcga
    17221 taattgcgcc aaacgagtta aatgtttgcg caagatttaa gcggaagtga ccactgcttt
    17281 ccggccctaa taccgtaaca aaagggtttg cggcagtttc cagacaacct aatccggctg
    17341 caataataaa taggccaact aaaaataagg tgtagttcat tatttctgca gcgggccaga
    17401 ataatgcagc gcccaaggca tataaaaata atccggtaat aatccctgct ttataactga
    17461 gttttttcat caatatccca gcagggattg ggataatgaa ataaccaaag taaaaggccg
    17521 attggatcag gccagcctgg aaatttgtca gcgtaaaagc ctgctggaat tgaggtaata
    17581 aaatgtcgtt aaggttattg gctaccgccc aaagaaaaaa cagtgagcac agcagcgcga
    17641 atggaataat gtaacttctg ctttgccctg catctttatc taccgcacgg taactctgcg
    17701 tttgtattga tgtgtttccc atagcatcct cttaggttca gaagcttaag cacgtaatcg
    17761 gttatgtttt caaaagtgca aaaccagcga gttcgggctt catatcagtg aatatttgcc
    17821 tgtctgaaat gaactcatgg attcattttc aaataaaaaa cgctctgtaa tatgacggcg
    17881 gtcacactta ttagctggaa ggtgacttta tgtgactacc atcactttaa taatcgaaat
    17941 ttcatgcccg tttcagaaaa taaatcataa aaaacacaaa ccagatgacc gtattaattg
    18001 tttatg
//
LOCUS       DAZGXZ010000103        17562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000103 DAZGXZ010000000
VERSION     DAZGXZ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.103"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..80)
                     /locus_tag="ACHKJF_003688"
     CDS             complement(<1..80)
                     /locus_tag="ACHKJF_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 domain-containing protein"
                     /protein_id="HFP0950971.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSE"
     gene            complement(207..938)
                     /gene="rlmB"
                     /locus_tag="ACHKJF_003689"
     CDS             complement(207..938)
                     /gene="rlmB"
                     /locus_tag="ACHKJF_003689"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP0950972.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1118..3559)
                     /gene="rnr"
                     /locus_tag="ACHKJF_003690"
     CDS             complement(1118..3559)
                     /gene="rnr"
                     /locus_tag="ACHKJF_003690"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP0950973.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLSKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3598..4023)
                     /gene="nsrR"
                     /locus_tag="ACHKJF_003691"
     CDS             complement(3598..4023)
                     /gene="nsrR"
                     /locus_tag="ACHKJF_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP0950974.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4228..5526)
                     /gene="purA"
                     /locus_tag="ACHKJF_003692"
     CDS             complement(4228..5526)
                     /gene="purA"
                     /locus_tag="ACHKJF_003692"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP0950975.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5630..5827)
                     /gene="yjeT"
                     /locus_tag="ACHKJF_003693"
     CDS             complement(5630..5827)
                     /gene="yjeT"
                     /locus_tag="ACHKJF_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP0950976.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5909..6913)
                     /gene="hflC"
                     /locus_tag="ACHKJF_003694"
     CDS             complement(5909..6913)
                     /gene="hflC"
                     /locus_tag="ACHKJF_003694"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP0950977.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6916..8175)
                     /gene="hflK"
                     /locus_tag="ACHKJF_003695"
     CDS             complement(6916..8175)
                     /gene="hflK"
                     /locus_tag="ACHKJF_003695"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP0950978.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8261..9541)
                     /gene="hflX"
                     /locus_tag="ACHKJF_003696"
     CDS             complement(8261..9541)
                     /gene="hflX"
                     /locus_tag="ACHKJF_003696"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP0950979.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9618..9926)
                     /gene="hfq"
                     /locus_tag="ACHKJF_003697"
     CDS             complement(9618..9926)
                     /gene="hfq"
                     /locus_tag="ACHKJF_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP0950980.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10012..10962)
                     /gene="miaA"
                     /locus_tag="ACHKJF_003698"
     CDS             complement(10012..10962)
                     /gene="miaA"
                     /locus_tag="ACHKJF_003698"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP0950981.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10955..12802)
                     /gene="mutL"
                     /locus_tag="ACHKJF_003699"
     CDS             complement(10955..12802)
                     /gene="mutL"
                     /locus_tag="ACHKJF_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP0950982.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12812..14149)
                     /gene="amiB"
                     /locus_tag="ACHKJF_003700"
     CDS             complement(12812..14149)
                     /gene="amiB"
                     /locus_tag="ACHKJF_003700"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP0950983.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14168..14629)
                     /gene="tsaE"
                     /locus_tag="ACHKJF_003701"
     CDS             complement(14168..14629)
                     /gene="tsaE"
                     /locus_tag="ACHKJF_003701"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP0950984.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14601..16148)
                     /gene="nnr"
                     /locus_tag="ACHKJF_003702"
     CDS             complement(14601..16148)
                     /gene="nnr"
                     /locus_tag="ACHKJF_003702"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP0950985.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16147..17286
                     /gene="queG"
                     /locus_tag="ACHKJF_003703"
     CDS             16147..17286
                     /gene="queG"
                     /locus_tag="ACHKJF_003703"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP0950986.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17269..17322)
                     /locus_tag="ACHKJF_003704"
     CDS             complement(17269..17322)
                     /locus_tag="ACHKJF_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950987.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3996 a         4996 c         4393 g         4177 t
ORIGIN      
        1 ctctcgctat ttgtcacatc aatattttgt tcaggtacag tttgcagcgc cgtcgccagc
       61 gccaacggat tccatgtcat atattattcc atatagattt agtttaaata ttaataaaat
      121 gaatatttgc agtacgtaat tatcttacca gctatagaca aaaaaaaacc atccaaatct
      181 ggatggcttt tcataattca gacgaactag ctgcgctgac gaaccgcttc aaataagcaa
      241 attccggttg caaccgaaac gttcagggaa gaaacacttc ctgccattgg gatgctgatc
      301 aactcatcgc aatgttcacg ggtcaggcga cgcatacctt caccttccgc gcccatcacc
      361 agcgccaggc gtccggtcat tttgctctga tagagtgtat gatccgcctc gcctgccgta
      421 ccgacgatcc agatattctc ttcctgcaac atacgcatgg tgcgcgcaag gttagtcacc
      481 cgaatcagtg gaacgctttc tgccgcgccg caggctactt ttttcgccgt agcgttgagc
      541 tgtgcggagc gatctttcgg cacaatcacc gcgtgaacgc cagcagcgtc cgcgctacgc
      601 aggcacgcgc cgaggttgtg cggatcggtt acaccgtcga ggatcagcag gaacggttga
      661 tcgagcgaag cgatcagatc cggcagatcg ttttcctgat actgacgtcc tggcttcacg
      721 cgggcgataa tgccctgatg cacggcaccg tcgcttttct cgtcgagata ttggcggttt
      781 gccaactgga taaccacgcc ctgagactca agggcgtgaa tcagcggtaa cagacgttta
      841 tcttcacggc cttttaaaat aaagacttcc tgaaaacgtt caggggcgcg ctccagcagg
      901 gcctgcactg cgtggatgcc gtaaatcatt tcgctcatta atgtactcgt tgtttacgtt
      961 ttgcctgatg cactacgttt atccggccta catgatctct gcaatatatt gaatttgcgt
     1021 gattatgtag gccggataag gcgttcacgc cgcatccggc atgaacaaag cgcattttgt
     1081 cagcaatcta accctcttct tttaaagagg gtattgatca ctctgccact tttttcttcg
     1141 ccgcacgctt cgctttggtc gctgcggcta ttttctgcgt tttcgccgat ggctttttcg
     1201 cttttctcgc gtctttcttc gccgctttcg gcttcgtttt tttctcaccg cggaaggcgc
     1261 tgtctggctc aaagtttacc tttttaccga cctgacgacg cttgccgcct tttttgcctg
     1321 catcgccttt tttcgctttc tcgcgcgccg ttttaccgac gttgcgcggt gcgcgttcac
     1381 tggagatcag actaaagtcg attttgcgct cgtccatatt aaccgcttcg acgcgaactt
     1441 ccacgcgatc gcccaggcga taagtctggc cgctggattc ccccatcagg cgttgcccta
     1501 cctggtcaaa gcgatagtag tcattgtcca gcgaagagac atggaccaga ccatcaatga
     1561 acaagtcgtc cagacggacg aagaagccaa agccagtgac gctggaaatt acgcctttaa
     1621 agacgttacc tacctggtcg agcatgaagt cacacttcag ccagtcagcg acatcgcgcg
     1681 ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg acccagttgc aacatctctt
     1741 ccatcgaata atggtagccg ccggtttcag tggtgttgcc ctgatgcccc tgctctttcg
     1801 acagcagata tttaatggcg cggtgcagcg tcaggtcagg ataacgacga atcggcgaag
     1861 taaagtgcgc ataggactgc aatgccagac cgaagtgacc acggttttct ggatcgtaaa
     1921 tcgcctgttt catcgagcgt agcagcatgg tttgcagcat ttctgcatca ggacggtcgg
     1981 caaccgactc cagcagttcc gcgtagtcac gcggttccgg cttgttacca cccggcagct
     2041 ccagccccag ctccgccagc actgaacgga aagaggtaat cgcttcggtg ctcggcttgt
     2101 cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac gaaacgcgcc gccgagatat
     2161 tcgccagaat catgcactct tcaattaatt tgtgcgcgtc gttacgctgg gtctgttcga
     2221 tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc ttcttcgctc tcaaatgaga
     2281 tcccaccgcg ttcttcacgg gctttatcca gcactttata gaggttatgc aactcttcga
     2341 gatgcttaac cagcggggcg tactgctcgc gcagatcctg atcgccctgc agaatatgcc
     2401 agactttggt gtaggtcaga cgcgcgtgag agctcatcac cgcttcgtag aatttgtagc
     2461 ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac catacacagg cggtctacct
     2521 gcgggttgag cgaacacagg ccgttagaga gcacttccgg cagcatcggg ataacctgcg
     2581 aagggaagta caccgacgtg ccacggttac gcgcttctct gtccagcggc gttggcggac
     2641 gcacatagta gctgacgtcg gcaatcgcga cccataaacg ccagccgccg ccgcgttttt
     2701 tctcgcagta aactgcatcg tcaaagtcac gggcgtcttc gccatcaatg gtgaccagcg
     2761 gtaaatcgcg caaatcgaca cggcccgctt ttgcttcttc cggcacttct tctttcagcc
     2821 cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg aatttcatgg gtacgcagag
     2881 cgatatcaac cgccatgccg gtgcccatat tatcgccaag gacttcgacg attttaccca
     2941 ccgctttggt gcggcgagtc ggacgctgag tcagttcgac tacgaccaca aagcccatcc
     3001 gcgcgcccat gatctgatcg ggcgggatta agatatcgaa gctcagacgg ctatcgtcag
     3061 gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc aacaatctgg ctggtttttg
     3121 gcaccagtac gcggacaata cgcgcttcac gacgaccttt acggtcagcg cccagcggct
     3181 gagccagcac ctgatcgcca tgaatgcagg ttttcatctg ctcgctggag agatacaaat
     3241 catctttacg cccttcaacc cgcagaaagc cgtagccatc acggtggcca ataacggtac
     3301 ctttcaccag gtcgaggcgt tccggcagcg catagcactg acgacgagtg aagaccagtt
     3361 gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc ttcaagctgc tcttcgcctt
     3421 caatgtgcag ttctaccgcc agctcatcac ggctggccgg tttttcacgt ttggttaaat
     3481 gttcgaggat aaattcccga ctagggatgg gattcgcgta tttttcagct tcgcgttcct
     3541 ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc tctgatgaag attttcgtca
     3601 ctccaccagc aataatttat aaagcggttg attctcttca accaaatcgg caagcgtgta
     3661 gttatccagt tccgtaagaa aactttgcac ggccttagaa agcgcctgtt tcaaccgaca
     3721 ggcaggtgta atgtggcaaa actcactgct gcaattcacc agcgataagg gctccagctc
     3781 gcgcaccaca tcaccaatac gtatcgcact cgccggttta cccaggcgaa tgccgccatt
     3841 ttttccacga acagcagtca cgtagccggc acgactaagt tgattgatta ttttgaccat
     3901 atgattacgg gagacgccgt agacgtcagt cacttcagaa atactggtca tccgcccttc
     3961 tggcaatgac gccatgtaga tcagcgcacg taatccgtaa tcagtgaaac tcgttaactg
     4021 cacatcaacc tcaaaaggga aatcgggaaa aaatacattt atattgatga taaaccagcc
     4081 acaagctgtg tcgctaattt atttcagttc ggggaaggaa aaaagcgagg atttaacact
     4141 gtgtcggata gcgtaatggc ttaaccgaca cagcaatgta tcacactgtt agcccggcag
     4201 gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac ggatcgcgca gaatcatggt
     4261 ttcagtacga tccggaccgg tagagatgat atcgatcggc acaccagtca gctcttcaat
     4321 acgcttgatg tagttcagcg ccgcctgcgg cagaccgcta cgatctttca cgccgaaggt
     4381 ggattcagac cagcccggca tggtttcgta aatcggctct acacctttcc agtcgtcagc
     4441 tgccagcgga gtggtcgtca cttcacgacc atccggcata cggtaagcca cacagagttt
     4501 cacctctttc aggccatcca gaacgtccag tttagtcagg cagaagccag acagggagtt
     4561 cagctgtacc gcacgacgaa cggcaacggt gtccagccag ccggtacgac gacgacgccc
     4621 cgtagttgcg ccgaattcgt taccctgctt gcagaggaac tcgccagttt catcaaacag
     4681 ttcggtcggg aacggacctg cacctacacg agttgagtaa gctttgagga tacccagaac
     4741 ataatcaaca taacgcgggc ccaggccgga accggtcgcc acgccaccag cagtggtgtt
     4801 ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc agcagcgtac cctgcgcacc
     4861 ttcaaacatg acgaaatcgc cacgctgacg cgcctggtcg agcaggtcag aaacgtcaac
     4921 caccatagaa gtcaggatgt cggcaacagc catcgtatca tccagaactt tctggtaatc
     4981 aaccgcttca gctttgtagt agttaaccaa ctggaagttg tgatattcca tcacttcttt
     5041 cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca acacgcagac cgcgacgtgc
     5101 tactttatct tcataagcag gcccgatacc acgaccggtg gtgccgatcg ctttcgcgcc
     5161 acgcgctttc tcacgcgcgt tatccagcgc aacgtgataa tcaaggatca gcggacatgc
     5221 ttcagacagc agcagacgct cacgaacggg gatgccacgg tcttccagtt ctttcatctc
     5281 tttcatcagc gcggccggag acagcacaac accgttaccg atgatgctgg ttacattctc
     5341 gcggagaata cctgatggaa taagatggag aacggttttt tcaccgttga ttacgagagt
     5401 atggcctgcg ttgtgaccgc cctggtagcg tacaacatat ttagcccgtt cagtcagaag
     5461 atcgacgatc ttacctttac cttcgtcacc ccattgggtg cccagtacga cgacgttgtt
     5521 acccattttt caaaatcacc gtttgcttaa aaatggattc taccatcgct ttttcagagt
     5581 tacagcactt tttgcactca aaaatgacct cgatcagtct gttttttgtt cagccaatcg
     5641 ttttcctcaa catgtagtag accacaacgc ccgcaaccac aagtccaccg ccaaaacgac
     5701 gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat cttcttccat gccttcgggt
     5761 aaagcatcgg ccctaaacct tccagtacca aaaccagggc aagcgccagc cagattgtcg
     5821 aattcattca ttatccttat agaaaaagaa aaccaccgac atccctgagg atgcggtggc
     5881 tttattgacc tgtaccgcag tcgttatatt aacgcgttgc ggaagtcggc gtcttcatgt
     5941 agcggaagaa atcgctatcc gggctcatga ccatcacgtc ctgattgcca gagaagctgt
     6001 tctcataagc acgcaggcta cggatgaatg cgtagaagtc cggatcttta ctgaatgcat
     6061 cagcaaacag tttggctgct tcggcatcgc cttcaccacg catgatgcgg ccctgacgct
     6121 cagcttctgc cagcgttctg gtcacttcat agtcggcagt cgcgcgcagt ttttccgctt
     6181 cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc acgctcggcg cgcatacggt
     6241 tgtagatcgc ttcagacact tcggtcggca ggttgatctg cttaattcgc acatcgacaa
     6301 cctcaatacc cagcgccgcc atactgttcg ggttgatgac cggaactttg cccttcgtct
     6361 cagccgttac gcgctctgcc gcttcggcaa tggcgttatc tgccgccggg gtagtaactt
     6421 catcttctgt acccgcagaa ccggagttca gcgcgtcacg tacttcgagg gtcagacgac
     6481 cacgggaatc ggtgacgata tctttcacgt ccaggcgacc aatttcagaa cgcagacggt
     6541 cagagaactt acgtttcaac agcacttccg cttgcgaaat gtcgccacca cccgttgcca
     6601 ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta agagtcgacg atcaggtctt
     6661 tcttctcttt ggtcacaaag cggtcggcct ggttgtccat ggtctgaata cgtgcgtcga
     6721 gcattttcac cgtttcaatg aacggtatct tgaaatgcag acccggctca taaaccagag
     6781 gtttgttgtc atcgtcacgc agtaccttac caaaacgcag cgtaataccg cgctcacctt
     6841 ctttgacgac aaagacagac atgtaaagca ctaccagcac gatgatgata atcgcgataa
     6901 ctgacttacg catcgttatt ccccctgacg ctggtagtcg ttacgctgcg cgttggcgcg
     6961 gcgttggtcc ataatatcgc cctgactggt ggacgacgtg ttgcttgctc cactggttgt
     7021 ggaggaagag gctggcggca gacgcagcag attgctggta ccgttatcgc tcttcgccgc
     7081 aggggcgtta ccgcctttca gcatctggtc taacggcaga accatcaggt tgccaccttt
     7141 atcgttaacc agcactttgc gggtgttacc caacactttt tccatcgtct cgatatacag
     7201 acgctcgcga gtaatttccg gcgcggcttt atattccggc agaagtttag caaagcgcgc
     7261 cacttcaccc tgagcttcca ggatggtctg ggccttgtac gcacgcgcct cttcgaggat
     7321 acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg gtatacgctt ctgcttcacg
     7381 aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg tcaaacgccg cttttacttc
     7441 ttccggcgga cgagcagcct ggaagttgac gtccagcagc gtgataccca tgtcatacgg
     7501 acggatagtc tcttccagtt cgcgctgagt atcgctacga atcacggtac gaccttccgt
     7561 cagaatgcgg tccatggtgt atttaccgat aaccccgcgc agggcgctgt cggtagcctg
     7621 acgcaggctg tcatccgggc tggtcacgct atacagatat ttttccggat tggtgacgcg
     7681 gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc gacgtcagca tcacaccaga
     7741 agcggccagt tcacgcacgg cttccacgtt caccggtttg acttcgtcga taaacgtcgg
     7801 tttccagttc agacccggct caaccagatg gctgaattta ccaaagcgtg ttaccacgcc
     7861 gcgttcggct tctttaatgg tatagaaacc actggccgcc cagataatga caatcgctgc
     7921 cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc gggccttgcg atgaactgcc
     7981 accgccagat ccggtgcctt taccgccgcc cagaccaccg agctttttgc tcagtttgcg
     8041 gaagatatca tctaaatcag gtggcccttg atcgcgaccg cctttgtttc catttccctc
     8101 agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg tcttgtccgt tattaccggg
     8161 ctgattccac gccatgtttg tgctccatat ttgttatgcg gtgatccctg ttgtctttcg
     8221 acgccagagg atatgactcc acgcttcaga cgctacgccg ttagatcagg taatcgatca
     8281 acgccggttc ttgtttacag aggcgacgcc agtcaacgat cggcatacga acttgcagac
     8341 ttacgctgcc gtcctcctcc atccactctt tttctattgc ctgaagctga taaaaacgac
     8401 ttctcagacg cccttcctgc ggcggcaaac gcaatgtatg ctgcgccacc tcgccggaaa
     8461 gccgctccgt caaagcctga aaaagctgtg gtatccccgc tccggtctgt gcggaaagcc
     8521 agacacggat cggtttgttc tcttcgtccc gatcaatacg cggttcgaaa tcttccagca
     8581 tatcgatctt gttcatcacc agcagggttg ggatctcgtg agcgtcgatc tcttcaagaa
     8641 ccgtattcac cgcttcgatg ttttcttgta cacgcacatc cgccgcatca atgacgtgca
     8701 gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt aaatgccgcc accagatcgt
     8761 gcggcaggtg gcgaataaac cctacggtat ctgcaagtac ggtttcaccg acatctgcaa
     8821 cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa ctggtctgcc gcgtagaccc
     8881 gcgcttcggt gatgcgattg aaaagggtag atttaccggc gttggtatat cccaccagcg
     8941 aaacagtagg aacgtcggct ttgatacgcg attgccgccc ctgctcacgc tgcttttcaa
     9001 ctctttccag gcgtgactgt atctgcacga tgcgattacg caacaaacga cggtcggttt
     9061 cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc tttctgtctt tcaaggtggg
     9121 tccagccacg caccaggcgc gtagccagat ggcgcagctg cgccagctca acctgcaact
     9181 taccctcatg ggtacgcgca cgttgggcga aaatatctaa aataaggccg gtgcggtcga
     9241 taacacgaca ctcgcacaaa cgctccaggt tacgctcttg cgccgggctc agggcatggt
     9301 caaaaagaac gaccgaagca cccgtcgctt tgacagcttc cgcaatttca actgctttac
     9361 cttcacctac aaaatacttt gggtgcggcg ctttacggct accggtaatc acctgcaatg
     9421 cttcgacacc ggcggaagag accagagatt caaactcctg gaggtcttcc atatctttgt
     9481 cttgcgtaaa atagatgtgt accagtaccg cctgctcacc agcatcataa cggtcaaaca
     9541 agcgtataac cctctaaata gatcagcggg gaacgcagga tcgctggctc cccgtgtaaa
     9601 aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct gttgcgcgga agtattctgc
     9661 gcgctgctac catgatggta gttactgctg gtaccgccac cggcgttgtt actgtgatga
     9721 gaaaccgggc gagacgggac aacagtagaa atcgcgtgct tgtaaaccat ctggctgacc
     9781 gtgtttttca acaggatcac gaactgatca aaagactcga tttgcccttg cagcttaata
     9841 ccattcacca aataaataga aactggaaca cgttcccgac gcagtgcgtt caggaacgga
     9901 tcttgtaaag attgcccctt agccattctc tcttttcctt atatgcttat ttgtactttg
     9961 aacctttcga ttctgaaaaa attgcgcacg atacgtctca attgtacaca ttcagcctgc
    10021 gatagcacca acaacctgta atacttcgtc gcgcgcctgt tctggttttt cactgtcaag
    10081 ccagtgaacc ccttcccaac cacgcagcca ggttatctgc cgcttcgcca actgtctcgt
    10141 ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt tcgccttcaa ggtaagacca
    10201 catctggcga taacccacgc aacgaatgga aggcaagtcc gtatgcaaat ctcctcgggc
    10261 aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac atctgatgaa aacgctgctc
    10321 aatgcgttga tggagcagtt cacggctcgc cggggcgatg gcgaactgat gcacctgata
    10381 cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa gttttacccg aaatgaaaaa
    10441 aacttccagt gcccgggaaa gcctttgtgg atcatttgga tgaatccttg ctgccgcaac
    10501 cggatctacc tcctgaagtt gacgatgcaa tgactcccaa ccttgctctg ccgcctgttg
    10561 ctcaattctg gcccgtactt ccgggtctgc cgacggtagc ggcgacaacc cttccagcaa
    10621 tgccttgaaa tacaacatcg taccgcccac taacagtggg atccgccccg ccgcggtgat
    10681 atcggccatt tccgccagcg catcgcggcg aaaatcagca gccgagtaag cctgcgacgg
    10741 atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac tcttcagcgt tcggcttcgc
    10801 cgtcccgata tccatccctt tgtaaataag ggcagaatca acgcttatca actctactgg
    10861 taaaatttta cgcagctcaa tggctaacgc cgttttaccg gaggccgtcg gccccatcaa
    10921 aaaaatcgcc ttaggcaggc tcgccttact gatatcactc atctttcagg gcttttatcg
    10981 ccggatgtaa atcaacagat tgtaacagac cacccggcgg cgttttcaca agttgcggac
    11041 ataaccgttc cacgtccgcc agcagggtta tggcctgtgc cattgaccac tgcgcatgtt
    11101 cgctcatcag atttcgtgca atccactgcg caatattgcc aggttcgaat acggactgct
    11161 tcgccaggta gcctatcagt tcaggaatca agatttgtaa attttgttgg cgtaagggta
    11221 aaggcacggc cctgatggtc acatgctgtg catctgactg gaaatcaata cccaattccg
    11281 ccagggcaga ctgcgctttt tctaatgccg atttttcttc gccagaaact tttagccgca
    11341 acggaataag cagcggctga gcgcaaactg gcgcttcacc cggcgtcagt tgtacctgac
    11401 gcagccaacg ttctgccact ggcaaggcta aaagtgaaat gttgccgtcg cgctccagca
    11461 acgcacagtc ggaatggacg atagtcagta cccgaccaaa actctgactg ttcgccgcaa
    11521 gtgcaggttc ctgcggttcc ggcgctttta atttttgcat cggcgcgggc gtttgcaaaa
    11581 gctggcgata cacttcacct tgctgtttct ggtagcctgg ctgcgcattc ggccaggggg
    11641 cagccggacg actgcctgat gctggcgcag gagtgtagcg cggagctacc ggctcacgag
    11701 ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg gttttccgga atggaacgcg
    11761 gtgcaggttg gggttcatcg tccagcggta gcggcgtttc cagttgctgt tgtagcacgc
    11821 tcagcacgcc ctgatagata aaatcatgca ccagacgcga ctgatggaaa cgcacttcgt
    11881 gtttggcggg gtgcacgttg acgtccacct gatgcggatc gatctccaga tacagcacaa
    11941 atgccggttg ctgatcggcc cccagtttgt cttcgcaggc ctggcggatc gcgtgattga
    12001 tcaggcgatc gcgcatcatg cgaccgttca cgtagcaata ctgaatttct gccagtgcgg
    12061 gcgtggtgtg atttggatcg gccacccagc cgcgcagggt gagatcgccg tgttgccatt
    12121 caatcgccag cgcttgttca agaaaagcgg tgccgcaaat cgcgcctaag cgccgttctt
    12181 tttgcccgcc ttccggcact gcgcggtact gacgcacaat tttaccgtta tgcgacaggt
    12241 tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat gatctcatca atgtggttaa
    12301 attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg ggtgttgtag aacagatcca
    12361 gcacctccag cgtcgtcccc acaggatgcg ccgccggttt taccgtcacg ttcatatcgc
    12421 gcccttcggc ataggcctgc caggcttcct gctgttctgc ggtgcgtgaa gtgagcgtca
    12481 ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg aaagcccagg ctgataatgg
    12541 cttcgagatc gtccagagag gcgattttac tggtggcatg acgagccagc gccagcgcca
    12601 gctcatcttt tttgataccg cagccgttat cacgaatgcg gataagtttc gccccaccgc
    12661 gttcgatatc aatatcgata cgcgtcgcgc ctgcatcgag gctgttttcc accagttctt
    12721 tgactaccga cgcaggtcgc tcgaccacct cacctgcggc aatctggttc gccagttgtg
    12781 gcggtaagac ctgaattggc atcaatcgtc cttagtttgg cagcgtgcga tctggcgtcg
    12841 tcaccgtact ggcagtttgt gccgttgccc cctgcggcgc agattgcatc ggatgcgcaa
    12901 ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag ctgttgttgg taatcgtcgc
    12961 tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc ggtttcgacc agtactgacg
    13021 ggatatccgg cgaacgcaga acgccgagac tcgcgtgttc tggtcggcgt ttatgtattt
    13081 cgccaatgcg ttgcaactga ctgatcatac tggtcgctac atcataccct acccgttggg
    13141 aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata ggggtcagac tgactgttcg
    13201 ccagcacatc acccgcccca cccagcagct ccgactgttt ctcgtgctgc tccagccagc
    13261 tggccatttc gctgttggcg cgacggttag agagcaccca tacggaagcg ccagtcgcac
    13321 tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa attggcgttt tgcttacgtg
    13381 ccacatcact gcgccccatc accgagataa agtaatcccc gtcacgggtt aaaacgccct
    13441 taaacatcgg atcgtcattg agcaaagtac gcaatttacg cgcgatggcg atggtgacat
    13501 ttttctcccg cgtaccaccg gggccgatag cgccagggtc ctgaccgccg tgtccggcat
    13561 caatagcgat gataatttta tcgccagtgt tagccgtcgc gcgcgctgcc ggacgcgtta
    13621 ccgtattact gctgataaca cccgtagtgc ggttactttc cgttttaaac ggattgcgcg
    13681 ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc aacgcgtttc gcaaccacgg
    13741 gcggcggagg aggaggtggc ggcgcatcgg cgttaatcgt aaagacgaca gtgtaattgc
    13801 tgccattctg ccgcttcacc gcttcggttt taccgttttc ggtaagatcg accaccagcc
    13861 gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc cttcaccaga ttattgccgc
    13921 tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt gatatcgagc gccacggtgc
    13981 gtttgctttg atggctaaac gcataatcag gatcgccaat aaaactcaac gttatccgcg
    14041 cctgttgatt accgttagaa acctgaatat cagagagcgt cgcggcaccc accggcgtgc
    14101 acagcagcag cagcgtcgct accaaccaat ttctgatgcg atacatcatc ccgccacctt
    14161 tcaaaggtta accggctaaa cgcgccagca acaattcacc cgcagaggaa accgcactca
    14221 cgcgcgcctc acggccttgt gcctgataat caatgtgtat ttcgacatcc gggtcaggaa
    14281 gaacaccagt accttgttgt ggccactcca ccaggcagat ggcatcgttg gcaaaataat
    14341 cgcgaatccc cataaactcc agctcctcgg gatcggcaag gcggtacaaa tcaaagtgat
    14401 aaaccattaa attgtcgagc gtatagggtt cgaccagcgt ataagtgggg cttttgacat
    14461 taccctgatg acccagagct tgtaaaaagc cccggctaaa ggtggtttta cctgcgccta
    14521 aatcgccata cagatagatt acggttgcgc catcgcaggc tttcgctacc cgctcgccca
    14581 ggtctaatgt tgcctgctca tcagggagcg gaattactcg attcatcatg gtttttatca
    14641 gtcacttccg ggttaacaat acgctgtagc gtggaaaaga gatcggttgc cagcatcccg
    14701 cgcgttccaa aacgcgccgc cagtacgtca gctgcagcac cgtgcgcgac acagcccgca
    14761 caggcggcat catacggcga cagtttttgc ccaagcaatg cgccaataat accagagagc
    14821 acatcgccca tgccgccgct cgccatgcct gcatttccgg catcaataat gcctaaagcg
    14881 tcaggatggg cggcgaccac ggttccggca cctttcagca ccgctacgcc gccataacgt
    14941 tgtaccagac gtttggcgca atgtaagcgg tcactttcaa tttcagcgac ggaacagcct
    15001 aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc gattgtgacg cttatcggga
    15061 ttgattgcca gcaggttcaa tgcatcggca tcccacaaca tcggtttgcg aaaattctca
    15121 actttttgca gtgctttttt cccccactct tgctggccca gaccgggacc aatcaccacc
    15181 acatcggccc attccaggct ttcggtaaga gagtccatcg tcagttcatg caccatcaat
    15241 tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc gggtcagtac tcggactaaa
    15301 ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa tagcccccgc cgtgccgtga
    15361 tcgccaccga taattaccag ccgcccgtga tcgcctttat gcgaagtcgg gcgacgcggt
    15421 tttagccagt gagaaagttg ttctgccgaa aaccgctgaa ttttcgtctc ctgacctgcc
    15481 agccaactat ccagccccag tgagtcaaaa tgcagttgtc cggtaacatc ccgcgctttt
    15541 ccagtgagca agcctggttt cagcgcaata aaagtgatgg tgtgatcggc gttgatcacc
    15601 gcgcctggcg tagcgccagt ttcagccagc aggccggaag ggatatcaac cgccacaatc
    15661 ggcgcaggat gggaattagc gtggtcgatt aactggctaa tggattcgcg gggcgcttgc
    15721 cgcaaaccgg taccgagcag cgcatcaaca atcagatcta ccgattcggg ccagacaata
    15781 ttcgaagcat ggatctcgcc acccgcgttt aaccatgctt cgcgtgccag cgcggcctct
    15841 tccggcaacg gtttgtcgct ctcctgggcc aacaacgtga cctcaatgcc gaccgctttg
    15901 gccagtcgcg cgaccacgta gccatcgccg ccgttattac catgaccgca cagcaccagc
    15961 cagtggcggg cgtcaggata cgccgaacga cacacctgga atgcggcctc gccagcgcga
    16021 agcatcagct catagagtgt gagccccagc acatctgccg cctcgcgttc tccgcggcgg
    16081 atatcgtcgg cgtaccagac ggtgtgtggt atacttacgg ggtttttctt cattgtatgg
    16141 tccgtcatgt cagagcccct cgatctcaat cagttagcgc aaaaaattaa acagtgggga
    16201 ctggaactgg gctttcagca ggtaggtatt accgataccg atctcagcga gtccgagccc
    16261 aaactgcaag catggctgga caaacaatac cacggcgaaa tggactggat ggcacgtcac
    16321 ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta cgctgcgcgt gatcagcgtg
    16381 cggatgaatt accttcctgc taacgccgca tttgccagca cgctgaaaaa ccccaaactc
    16441 ggctatgtta gccgttatgc gctgggccgt gactatcaca aacttctgcg caaccgactc
    16501 aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc tgaattttag accgtttgtc
    16561 gattctgcgc ctattctcga gcgcccgtta gctgaaaaag ctgggctcgg ctggacaggt
    16621 aagcactcac ttatcctcaa tcgcgaggcc ggttctttct tctttttagg cgaattgctg
    16681 gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag gatgcggtaa atgcgtggcc
    16741 tgtatgacga tttgcccgac cggtgccatc gtcgagccat ataccgtcga tgctcgccgc
    16801 tgtatctctt atctcaccat cgaacttgaa ggggcgatcc cggaagagtt gcgaccgtta
    16861 atgggaaacc gtatttacgg ttgcgatgac tgccagctta tctgcccgtg gaatcgctat
    16921 tcgcaactca ctacagaaga cgatttcagc ccgcgtaagc cgctacacgc accggaactc
    16981 attgagttat tcgcctggag cgaagagaag tttttaaaag tcacggaagg atcggcgatt
    17041 cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg tagcattagg caatgcccct
    17101 tgggatgaaa cgattttgac agcgctggaa agtcgtaaag gtgagcaccc acttctcgat
    17161 gagcacatag cgtgggcgat tgcgcagcaa atagagagac gaaatgcgtg catagtcgaa
    17221 gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg aaaaagggtt accgcgtgac
    17281 gcctgattca tccacagctt gtgaataaaa ataaaaacgc attgcaattc aagacgaata
    17341 aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat ttttaactta tctatcaata
    17401 ggttaaaaaa atactattca ccgtgcgaag tttctttgaa attaagatta cagcctgagg
    17461 ctgtggataa ctctgtttac aagagtttgt cagagacaac gaaaaccagc aataacgcat
    17521 cgcatcgctg tggatatttt attgagagaa gaatttggag cg
//
LOCUS       DAZGXZ010000104        17350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000104 DAZGXZ010000000
VERSION     DAZGXZ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.104"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHKJF_003705"
     CDS             <1..207
                     /locus_tag="ACHKJF_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HFP0950988.1"
                     /translation="EAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEIKH
                     FRRWLGKLEKDGVIYVDGDDVGPL"
     gene            410..661
                     /locus_tag="ACHKJF_003706"
     CDS             410..661
                     /locus_tag="ACHKJF_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950989.1"
                     /translation="MTQKRRDRTQPKYKALDMTEHALKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            658..963
                     /locus_tag="ACHKJF_003707"
     CDS             658..963
                     /locus_tag="ACHKJF_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950990.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            973..1383
                     /locus_tag="ACHKJF_003708"
     CDS             973..1383
                     /locus_tag="ACHKJF_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0950991.1"
                     /translation="MTAQIAAYGRLVDDPQVKQSSKGTPMTLARMAVSLPCSQAHDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAARPGGNRRKATGTQGNQPPAGGDDPYGDDIPF"
     gene            1394..1666
                     /locus_tag="ACHKJF_003709"
     CDS             1394..1666
                     /locus_tag="ACHKJF_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0950992.1"
                     /translation="MAHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHQVTMRRL
                     ECSRKAQRPPEPPDNFGDLRKAVDRTYAEMGMDGVSDEIWRNYTDS"
     gene            1954..3111
                     /locus_tag="ACHKJF_003710"
     CDS             1954..3111
                     /locus_tag="ACHKJF_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0950993.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            3151..3723
                     /locus_tag="ACHKJF_003711"
     CDS             3151..3723
                     /locus_tag="ACHKJF_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFP0950994.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWVDLAGV"
     gene            3725..4936
                     /locus_tag="ACHKJF_003712"
     CDS             3725..4936
                     /locus_tag="ACHKJF_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0950995.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            4933..5271
                     /locus_tag="ACHKJF_003713"
     CDS             4933..5271
                     /locus_tag="ACHKJF_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0950996.1"
                     /translation="MNIGRLRDRITIQTLKQTRAMTGEILETWEDGHTLWASVNMISS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            5268..5564
                     /locus_tag="ACHKJF_003714"
     CDS             5268..5564
                     /locus_tag="ACHKJF_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0950997.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGVY"
     gene            5564..6004
                     /locus_tag="ACHKJF_003715"
     CDS             5564..6004
                     /locus_tag="ACHKJF_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0950998.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            5988..6170
                     /locus_tag="ACHKJF_003716"
     CDS             5988..6170
                     /locus_tag="ACHKJF_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0950999.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            6293..6649
                     /locus_tag="ACHKJF_003717"
     CDS             6293..6649
                     /locus_tag="ACHKJF_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFP0951000.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            6633..8294
                     /locus_tag="ACHKJF_003718"
     CDS             6633..8294
                     /locus_tag="ACHKJF_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0951001.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLESPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKTTGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRRRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            8308..8589
                     /locus_tag="ACHKJF_003719"
     CDS             8308..8589
                     /locus_tag="ACHKJF_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951002.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMH
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVEVYREKEKITYHWKKPERLRR"
     gene            9349..10137
                     /gene="thiM"
                     /locus_tag="ACHKJF_003720"
     CDS             9349..10137
                     /gene="thiM"
                     /locus_tag="ACHKJF_003720"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP0951003.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            10134..10934
                     /gene="thiD"
                     /locus_tag="ACHKJF_003721"
     CDS             10134..10934
                     /gene="thiD"
                     /locus_tag="ACHKJF_003721"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP0951004.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            11257..11816
                     /locus_tag="ACHKJF_003722"
                     /pseudo
     CDS             11257..11816
                     /locus_tag="ACHKJF_003722"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
     gene            11868..12614
                     /gene="yegW"
                     /locus_tag="ACHKJF_003723"
     CDS             11868..12614
                     /gene="yegW"
                     /locus_tag="ACHKJF_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0951005.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(12588..13553)
                     /gene="yegV"
                     /locus_tag="ACHKJF_003724"
     CDS             complement(12588..13553)
                     /gene="yegV"
                     /locus_tag="ACHKJF_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0951006.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(13550..14554)
                     /gene="yegU"
                     /locus_tag="ACHKJF_003725"
     CDS             complement(13550..14554)
                     /gene="yegU"
                     /locus_tag="ACHKJF_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP0951007.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAELENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(14551..15828)
                     /gene="yegT"
                     /locus_tag="ACHKJF_003726"
     CDS             complement(14551..15828)
                     /gene="yegT"
                     /locus_tag="ACHKJF_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP0951008.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            16085..17137
                     /gene="fbaB"
                     /locus_tag="ACHKJF_003727"
     CDS             16085..17137
                     /gene="fbaB"
                     /locus_tag="ACHKJF_003727"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP0951009.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
BASE COUNT         4526 a         4312 c         4759 g         3753 t
ORIGIN      
        1 gaggcaggca acaagacgga aaaccatacc gcgctatggg ggtgtatccg ttcacgcaca
       61 cagaacggcg acaagtgcac gatcccgctg ttacgtgatg acatgaaaaa actggggtac
      121 gaaataaagc acttccggcg ctggctgggc aagctggaaa aagacggcgt tatctatgtt
      181 gacggcgatg atgtagggcc actgtaaaaa gtggggggca aaagtggtgg gtatggggaa
      241 tttaacaaaa tttaaacacg atcccccact ttttgacctg tatacatccc aaaaagtggg
      301 gagcaaaaaa atccttataa aacagcatga taaaaattac caatcccacg cctttaacga
      361 gatggggaat tgtgaaaagt ggggagtaaa agtgggtata ggtggggaga tgacacaaaa
      421 acgcagagac agaacacagc caaaatataa agcgttagac atgacagagc acgctttaaa
      481 ggtggcaatc agaacgatag accgccacgc gggggaagga tacgcgaagg agcatcccga
      541 actgataagc gcattcatga ccacgacggc ggcaaatttt gccacgctga cagagcggga
      601 gattgccgaa gcggaacagg taacaacaat caacgttaaa accggagagg tggaatcatg
      661 agcgaaccaa aatttggtga aaaattttac aagcacaacg gacggatcac gattctgcaa
      721 ataagcgcag ctacaccagg ctggtgggtt gaaacagacg aaggatcctc acccgtggca
      781 tcatgggcgc tttgtgtgct tagttaccct gaccgcgatg tgtatcagga tattctgcct
      841 gtgatatcca ctgacaaagg catgaaaccc gtagacatca aaaacatggg gtttcagtgc
      901 gtaatgctca ctgaaaaaat gatggaagag atgaaaaaga acaatgccgg atcgcttcac
      961 tgaggtggca atatgacagc acaaattgca gcttacgggc ggctggtgga cgacccgcag
     1021 gtaaaacaga gcagcaaggg cacaccgatg actctggcgc gtatggcggt atcgttgcca
     1081 tgcagccagg cacatgacgg acaggcgacg ttatggttat cggttatcgc atttggcaaa
     1141 caggccgatt tcctggctaa acatcaaaaa ggcgatgttg ccagcgtatc cggcacgatg
     1201 caggtaagcc agtggaccgg acagaacggg gaaacacggc aggggtatca ggttattgcg
     1261 gacagcgtaa tcagtgcccg cgcggcacgt cctggcggga acagacgcaa agccacaggc
     1321 acacagggta atcagccacc agcgggaggt gatgaccctt acggtgatga tattccgttc
     1381 tgagggggtg acgatggcac atgaccgcat agcggaggaa ctcgaggcaa aaggctttta
     1441 ccggagggcg gcggcgcgat ggggtgaagt catgcaactg gtggagacag acaaggaacg
     1501 gcatcaggtt acgatgcgac ggctggaatg ttccaggaag gcacagaggc caccggagcc
     1561 gccggataac ttcggagacc tgagaaaggc agtcgatcgc acttatgctg aaatgggtat
     1621 ggatggtgta agcgatgaaa tatggcgtaa ttacacagac agctaatcaa cagccggagc
     1681 aatccggctt tttgttattt tttgtaaatt atttgttcgt ggttgttcca cgttgttcac
     1741 tgagcggatc ggcatatttt acccgaactg aatcatgatt attctcgccc gtggtgccag
     1801 gacgctgggg ccactttccc gcctgttaat gtgctcgcca atattcatta ccaggcggga
     1861 aaacgatcgg tgcgattact gatttcctta tgaaaaacgg ttgagtgttt gccgcgtcct
     1921 ggagttcctt acttaacccc aggacttttt tttatgccga gaataatcga attacgccag
     1981 cagaaaaccg ccattaaaaa tcagatgcgc gacatgctgg agaacgcgga aaaagaaaac
     2041 cgcagtctta acgatgctga gggcgcaaaa tttgacgaat tacgcgctaa agctgaatcc
     2101 ctcgataaag acatttcccg ccttgaagcc attgcagacg aagagcgcag caagccaggt
     2161 aaaagcagtc agaccactga ccccgcagaa ctacgaaact acattctgac gggggaaacc
     2221 cgcgcattaa gtacaggcgt tcccgctgat ggtggttata ccgttatccc cgaactgaac
     2281 accgaaatca tgcgaatgct ggcggatgaa tccaccatgc gccgcatctg taccgtgaaa
     2341 aaaatcagca gcaacgagtt taagcagctt gtttccgctg gcggtgcgac cgttaaccac
     2401 ggtgaagagg gtaaggcacg cgaacagacc agcaccccgc agattaacga ggtgagcatt
     2461 aagctgtatc cggtctatgc gtacccgcgc accacacagg aaatcgtgga tttttccgat
     2521 gtggacatcc tttcatggct gacgggtgag attggcgaca ccttcacgga aaccgaagaa
     2581 agcgatctgg ttgtgggcga cggtgacaaa aaagcaaaag gttttttatc cgtaccccgt
     2641 gcagagaaga acgacaaaga gcgtgatttt ggtacgttgc aggtaattaa accttccgaa
     2701 tctctggcgt ggacatctgc ggacccgttg atcgacctga aatttgcatt acgtaaaaaa
     2761 taccgcaaaa acgcggtctg ggtggttaac tccacgacgg cggcaaaact tcagaaggtg
     2821 aagaacgcga acggtgatta catctggcgc gaccgtttac aggcgggtga tcctgatacg
     2881 ttgctgggcc ttccggtcga atatctggag tttatgcctg ataacgttat tgccctgggt
     2941 gacttcaaac gcggttacta cattgttgat cacgaaacag gtgttcgcac cagaccggac
     3001 aacctcacag agccgggctt catcaaaatt ttcacgcaga aatatttagg cggtggcgtg
     3061 gtggattcga acgcgatcaa gattctggaa ctgccacagg acgacgatta acagcataca
     3121 gaaggggctt aaaagcccct ttagtgtttt atgggtgaaa aaattatgaa gagtatggaa
     3181 atccggtcat cggaaatcac caccagcgga gccggtacgc tgacgggcta cgttgttcgc
     3241 tgggataagc tttcagaact gttatggggt gagttttacg aaaaattcca gcggggagcg
     3301 tttactgaat ggcttgcagc gggtaatgac gttcgcggcc tgtatgagca tgaccacagt
     3361 atgttactgg ggcgtacccg ttccggcacg ctgaaactgg aagaggacga aacagggtta
     3421 cgctttgaac tgaccccacc ggataccagc acagggcggg acgttatcga actggttaaa
     3481 cgtggtgata tatccggcat gagctttggc tttcgttccc gtaaggatgt atgggatacc
     3541 acaacagatc catgcgtgcg caccgtgctg gtggcggaac tgtacgaaat taccgttaca
     3601 tccgttccgg cttaccctga ttccggcgtg gagctggccc gccgttccct gtatgagcag
     3661 caccccgaaa aaatgccgcg tgcggataat cgccgctggt gggtggattt agcgggggtg
     3721 tgatatgtgg cctttcagaa gaaaaaaaga gcagcgcagc atgacgcttg atgaatttat
     3781 ggcgctggct ggcacatcga acacgggggc gggtgagtac gtatcatcgg ggacagcgga
     3841 atcactgccc gccgtcatga acgccgtcac ggtcatctct gaggcggtgg ctaccatgcc
     3901 gtgttacctg tacctggtac gcaatgagaa ggggaaggag gcccgcgagt ggcttgattc
     3961 tcatccggtc gatcacatcc tcaacgagcg cccgaacgca tggcaaaccc cctaccagtt
     4021 taagcgaatg atgatccgcc actgcctgtt aaacggtaat gcttatgcgg tgattcagtg
     4081 ggggcgtgat ggttttccgg cggctttaca tccttacccg ccgcagtcgg tgaacgtgga
     4141 gcagacaggc gaacacaact ggcgctactg catcactgac gcctacaccg gaaacacccg
     4201 caactattta ccgtgggaag tacttcacct tcgttactcc acggatgacg gctttatggg
     4261 gcgctcacct gtaaccatct gccgcgaatc gctggggctt gggctggccc aacaacgcca
     4321 cggcgcgagc gtgatgcgtg atggcatgat ggcggcaggg gttatcacgt caggcgaatg
     4381 gctggacggc gtgaagggca aacaggcatt agccgcactg gaacgctaca aaggggccag
     4441 aaacgccgga aaaacgccca tccttgaagg gggtatgagc tatcagcagc tgggcatgag
     4501 taatcaggat gctgaatggc tggcctcccg tcgcttcacc attgaagaca tcgcccgaat
     4561 gttcaacgtc tcgccgattt ttttgcagga atacagcaac agcacctaca gcaatttcag
     4621 cgaggcaagc cgcgcatttc tcaccatgac aatgcgcccg tggctggcga actttgagca
     4681 gcagataaaa aacgccctgc tggtggcctc gcctgtacct ggtatccggt atcaggtgga
     4741 gtttgacagc gcggacctgt tacgggccac acctggcgaa cgctttgcca cctatgagcg
     4801 cggcatcaaa tccggcgtta tgtgtccgaa cgaagcccgc gaacgtgaag gactgtcacc
     4861 gcgtgatggt ggtgatgagt tcagccaggc atggaaacag gaagtaaaaa tcagcgaggg
     4921 agaaaaaccg gaatgaacat agggcgactg cgtgacagga taacgattca gaccctgaaa
     4981 cagaccagag ccatgacggg cgaaatactc gaaacgtggg aggacggtca cacactctgg
     5041 gcaagcgtga acatgatcag cagcaaggag gccatttcat cgggcgcaga gctggcgatt
     5101 ggtaccgtaa ggatctggat acggtaccgg aaggacatca acgccaccag tcggataaag
     5161 gtcagtacgg ggccgctggc ggggcgtgta ctgaatatca tcgggcagcc gctgccggat
     5221 gccgccagga cacgccttga aattctttgt cgtgagggcg cggaaaaatg acagaagaac
     5281 ttatcaccct ggaagaagtg aaactccatt gccgcatcga tggcgacgat gaagaccagt
     5341 taatcagcgg atacattgcc gcatcgcttg aggcgtgcca gatacacata ggcaggcgct
     5401 ttgatgacgg gctggagttc acgccagcca taaagattgg ctgcatgatg tttatcgctc
     5461 acctgtatga aaatcgccag attgtcgcgg ataacgcaaa aacacgcgta cccatgacga
     5521 taggcgcact ctggacggct taccgtgatg tgggggtgta ctgatgccgt ggcaaccatt
     5581 aagacgatgc acagaaccag gctgtaacag gcgcgttaaa tccggcaagt gtgaggagca
     5641 caggcgggcg gcatggcgtg cagaggatgc cagacgagga caccgccgcg cgcgtggata
     5701 ttccagacag tgggacaaat accgcgccat gtatctgagt aaaaacccgt tatgcgtgcg
     5761 atgccttgag aaggggatat atacgcccgc cgtggtggtg gatcacatta tcccgattaa
     5821 tggcggtgat gatgttctct tctggcccga atggaaccat caaccattgt gccaggcgtg
     5881 tcataaccag aaaacgaaat ggcttgatcc ggcaacaaaa agcaagcgtg ccgcaggtgg
     5941 atttcgtgaa gaggaagaac gggccgctaa ccgcaataac tggatgtatg acgctgatga
     6001 atgagcggga gcaaaaccgc cttatccgtg gacttataag gcagcgtgac gcatggaaga
     6061 cacaggaaac agggcataaa gataaagcgt caggacgcgc agaacgcatc acagcgaagc
     6121 gattaaccga ccgtgaccgc gaggttatgg aatgtttccg caatcgctga tgaggctgta
     6181 tgacggggtg gggggcgttt tcaggaccaa cccgaccccg ccgggcaccg aacgcctcct
     6241 caaattttta tgcacgggaa ttttttgaaa aataatctgg cgaaaaataa gcatggcaag
     6301 accaccgaaa gcccccgcct acctggatga tatcgccgtg aagcagtggc gggaaaaatc
     6361 taggcagctt gcggaacggg gagacctgac ccccgccgac tggagcaatc tggaactgta
     6421 ttgtgtcaac tactccattt accggaaagc cgttgcagac cttgcggcgc gcgggttcag
     6481 cattgttaac agtcagggcg gtgagagcag aaaccccgca ttaagcgcaa aatccgacgc
     6541 tgaaagagtg atgataaaaa tggcctcttt gctcggtttt gacccgataa gccgccgtaa
     6601 aaatccaccg gaaacagaag aagaggacga gcttgaccgc ctggaataag tacgcagaag
     6661 acgtaaaaac gggcaaaatt ccggcctgta aacggctgaa acaggccgtt aaacggtact
     6721 tttcggacct tgaaagcccc ctttacacgt tcgatcgtga ggttgtggag cggtttattg
     6781 ccttttccag ggtgtgcccg cacgtaaaag ggccgatgcg tggcagaccc attgagctgg
     6841 agccgtggca gcagttcgcc tttgcgtgca tcctcggctt taaggttaag accaccggac
     6901 ggcgcaaata cacgagcgcc tttatcgaag tgccgcgcaa aaatgcgaaa tccacgaccg
     6961 ccgcgattct ggctaactgg tttctgatta tggagaacgg gcagcaggat atttacaccg
     7021 cagcggtgag ccgtgaccag gcgcggatcg tttttgatga tgcgcgtcag atgtgccttt
     7081 tatcccgacc gttacgcagg cgggtgaata ttcaggccca caaggtgatg cacccgaaaa
     7141 gcaacagcct gttaaagccg ctggcagcaa aagcggcaac catcgaggga acaaacccca
     7201 gcctttccat cgtggacgaa taccacctac accccgataa cggcgtttat tccgcgcttg
     7261 agctggggat gggcgcacgt cccgaagggc tgttatttgc cattaccaca tcgggcagta
     7321 acgtcgtttc agcctgtaaa cagcactatg attattgctg ccagatcctg gacggcgaag
     7381 aggtcaacga ttcaattttt gtgctgattt acgagctgga cgacgaaaac gaggttgatg
     7441 atccggcgat gtggataaag gctaacccta acatcgatgt ttccgtggat cgtgaaaaac
     7501 tggcctcaac catccagaaa gcgcggggta ttccgtcgca atgggtggag atgatgacaa
     7561 agcgatttaa tatctggtgc cagggggcta ccccgtggat gggtaacggc gcatgggctg
     7621 agtgtgccgg aacgttcacg gaggaagatt tacatgggca ggagtgttac gcggggctgg
     7681 acttatcatc aaccagcgat atttccagcg tgtgctatgc ctttccggtc ggtaaaaata
     7741 tcatgctggt ttcccgtcat tatctgccgg agttccagct acagaacccc gccaataaaa
     7801 accgcgctgt ctatcgccag tgggctaaag cgggctggat acgcacaacg ccaggtgact
     7861 gcattgatta cgaccggatc agagatgaca tcatgcagga tgcggagaaa tttaatatca
     7921 ggctggtggg ctttgatacg tggaacgcca cgcatctcag gacgcaatta cagggggctg
     7981 gttttgaggt ggagccgttc ccgcaaacct acctcagatt cagtccggcg gcgaaatcgt
     8041 tcgaagtttt tgttaaccgc agggtgattg tgcatcgtgg cgatccggtg ttgtcctggt
     8101 cgatgagtaa cgtcgtgatg cagagtgacg cgaacgccaa tatcaagccg aacaagaaaa
     8161 aatcaccgaa caagatagac ccgagcgtgg cggcgctgat ggcgtttggt acattccagg
     8221 cagagcatga ggattttgct tttgatataa gcgacagcca ccgccagaaa ctggaagaat
     8281 ttagcggggt gtaatgaggt cagcagcatg acagaagccg aaatactgcg attaatccgc
     8341 cgcgtctctg gaatcagcca gcaggctgac gaacagacca cgcagccgga cagcgtgaca
     8401 gccgaaaatt atgcgcgtgt tgttgctgag gtgatgcacc gtgatggtat ccagcttaat
     8461 gatgcggata tgcgcgacat acggatccgc gttctggaaa tgctggccta caatcgccgc
     8521 gttgaagtgt atcgggagaa ggagaaaatc acgtaccact ggaagaagcc ggagcggtta
     8581 cggcggtaac ttgttgatta aaaggcggct taattctgag ctgggtagct gacggcggta
     8641 actgttttat attcccgata gcgcaaaaat gcgcggtcgg gcagaatgac caccacctca
     8701 atcgtgacgg agagtgacaa ccagtgacac accgtgacaa aaagacggtt taaccgttca
     8761 ttaaaaatcg acgtgatttt gtgctatcag gatgacgtgg acaacgtcat tttttagcga
     8821 aaaattctga ttaaggtcac tggctgaatg gtcataatga ccacgcagac catcgaggca
     8881 accatagcga tcatcgttac ggttggagct tttccgccat tggtggaaaa ggtgcacatc
     8941 tggatcatca ttacggtgat caagggtatg taaaacccac cagcctgatt aaaggttaac
     9001 cggaaaaaaa atccggtagc cccgttatgg ggatccccat atcggggaac atccaccagc
     9061 ttcataacgg atagccggaa aatgataacg gttaagcaaa accgccagca aatttccctt
     9121 ttgtttgtta ttgttcgtca ttgttcgcag agcttcaacg aaatgtgtgg tcagttgtgt
     9181 ggtcagtttt taggttttta attcttgtgt gctaaaaaac ttattaatta tcaactgaca
     9241 acaaaaatta gtaagaaata cccgtatcac ctgatctgga taatgccagc gtagggaagt
     9301 cacggaccac caggtcattg cttcttcacg ttatggcagg agcaaactat gcaagtcgac
     9361 ctgctgagtt cagcgcaatc tgcgcacgcg ttacaccttt ttcaccaaca ttcccctctt
     9421 gtgcactgca tgaccaacga tgtggtgcaa acctttactg ccaatacctt gctggcgctc
     9481 ggtgcatcgc cagcgatggt tatcgaaacc gaagaggcca gtcagtttgc ggctatcgcc
     9541 agtgccttgt tgattaacgt tggcacactg acgcagccac gcgctcaggc gatgcgtgct
     9601 gccgttgagc aagcaaaaag ctctcaaaca ccctggacgc ttgatccagt agcggttggt
     9661 gcgctcgatt atcgccgcca tttttgtcat gaacttttat cttttaaacc ggcagcgata
     9721 cgtggtaatg cttcggaaat catggcatta gctggcattg ctaatggtgg acggggagtg
     9781 gataccactg acgccgcagc taacgcgata cccgctgcac aaacactggc acgggaaact
     9841 ggcgcaatcg tcgtggtcac tggcgaggtg gattatgtta ccgatggaca tcgtatcatt
     9901 ggtattcacg gtggcgatcc gttaatgacc aaagtggtag gaactggctg tgcattatcg
     9961 gcggttgtcg ctgcctgttg tgccttacca ggcgatatgc tggaaaatgt cgcatctgcc
    10021 tgtcactgga tgaaacaagc cggagagcgc gcagtcgcca gaagcgaggg gccaggcagt
    10081 tttgttccac atttccttga tgcgctctgg caattgacgc aggaggtgca ggcatgaaac
    10141 gaattaacgc tctgacgatt gccggtactg atcccagtgg tggtgcgggg attcaggccg
    10201 atcttaaaac cttctcggca cttggcgctt atggttgctc agttattact gcactggtgg
    10261 cgcaaaatac ccgtggcgta cagtcggtgt atcgcattga gcctgatttt gtcgccgccc
    10321 agctcgattc ggtgttcagc gatgtgcgaa tcgacaccac taaaatcggt atgttggcgg
    10381 aaaccgatat tgttgaagcg gtggcagaac ggttgcaacg ttatcagatc caaaacgtgg
    10441 tactcgacac cgttatgctg gcaaaaagcg gcgacccgct gctttcacct tcggcggttg
    10501 ctacgctgcg cagtcgatta ttgccacagg tttcattaat aacgccaaac ttgcccgaag
    10561 ctgccgcctt gctcgacgcg ccacacgcgc gcaccgaaca ggaaatgctg gaacaagggc
    10621 gatcgctgtt ggcgatgggc tgtggcgcag tgctaatgaa aggtggtcat ctggatgatg
    10681 agcaaagccc ggactggctg tttacccgcg agggtgaaca acggtttacc gcaccgcgca
    10741 ttatgaccaa aaacacccac ggcactggtt gtacgctctc tgcggcgttg gctgcactac
    10801 gcccgcgcca tacaaactgg gctgacaccg tacaggaggc aaaaagctgg ctttcatcgg
    10861 cgttagccca ggccgacacg ctggaagttg gtcacggtat tggtccggtt caccacttcc
    10921 acgcctggtg gtgagcaccg tactatagct gctttggtta ttgtcagcct ttggcactga
    10981 cattcaggga tgaaaccaat gcagctaaga attaccagtc gaaaaaaact cacctctcta
    11041 ttatgcgctc ttgggctaat ttccatcgtt gcaatttatc cgcgtcagac ggtgaatttt
    11101 ttctattcga cagcagttca gattacagac tacatccatt tctacggtta tcgcccggtt
    11161 aaatctttcg ctattcgcat tcctgccagt tacaccattc acggaataga tgtttctcgc
    11221 tggcaggagc ggatcgactg gcagcgggtg gcaaaaatgc gcgacaacgg tatccgctta
    11281 cagtttgctt ttattaaggc gacggaaggc gaaaagctgg tggacccgta tttttcgcgt
    11341 aactggcaac taagccgcga aaatggcctg ctgcgctggg cgtatcatta tttctccccg
    11401 tcggtagcag cgccggttca ggcacgatta tttctgcaaa cggtggattt ctcacaaggc
    11461 gatttccctg cggtactgga cgtagaagag cggggaaaat tatcggcaaa agaattacgc
    11521 aagcgggtaa gtcagggcta aaaatggtcg aaaaacgcac gggaaaaaag ccgattattt
    11581 actcaggagc cgttttttat cacacgaatc tggcgggcta ttttaatgaa tatccgtggt
    11641 gggtggctca ctactatcaa cgtcgtccgg acaatgacgg catggcctgg cgtttctggc
    11701 agcattccga ccgtggacag gtagatggca tcaatggtcc ggtggatttt aatgtgttta
    11761 atggcacggt ggaggagttg cagggattcg ttgatgggat taaagaaacg ccttaaataa
    11821 gaagcagtga tatactccca ccagactccc tggcaggaaa aagacaaatg gaacaagcgc
    11881 atacccagct gattgcccaa ctgaacgagc gtattttagc tgcggataac acgccgcttt
    11941 atattaagtt tgccgaaacg gtaaaaaatg ccgtgcgcag cggggtgctg gagcatggca
    12001 atattttgcc cggtgagcgt gatttaagtc agttaaccgg cgtttcgcgc attacggtgc
    12061 gcaaggcgat gcaggcgctg gaagaagagg gtgtggtgac gcgttcgcgc ggttacggca
    12121 cgcagatcaa caacatcttc gaatactcgt tgaaagaagc gcgaggtttt tctcagcagg
    12181 tggtattgcg cgggaaaaag cccgatacgc tatgggttaa caagcgtgtc gtgaaatgtc
    12241 ccgaagaagt cgcgcagcag ctggcggtcg aagcaggaag tgatgtcttt ttgcttaagc
    12301 gtattcgcta tgtcgatgaa gaagcggtat cgattgagga atcgtgggtt ccggcgcatt
    12361 taattcatga tgttgatgcc atcgggattt cactttatga ctatttccgc agccagcaca
    12421 tttacccaca acgtacacgt tcccgcgtta gcgcccggat gccggatgcc gagtttcagt
    12481 cacatattca gttagatagc aaaataccgg tgctggtgat caagcaagtg gcgcttgacc
    12541 aacagcaacg gcctattgag tacagcatta gccactgtcg cagcgatcta tacgtttttg
    12601 tgtgcgagga gtagttcctc gcgcgttggc gcacaatcac cgccccgatg cccgacaacc
    12661 cacgacgcca ctgcattgcc cagtaatacg gcatccgcca gtggcagacc agaggccagc
    12721 ccggcaagta cgccacctgc atgactgtcg cccgccccaa tggtgtctac aacttgcgtt
    12781 ggaaatgccg gaatgcagcc agaagcgtcg ttgctgaaat accatgcacc ctctttatcg
    12841 agacgaacga tcaacggcgc ggcaaatttc tcctgccatt gcttgccaag tgttgttatc
    12901 tctgcggata aagcaaaacg ttcggcggca atctcagcct cttgacgatt gagtgatact
    12961 aaaggtcgac aggccatgat ccgcgccagt aatgcgtccg ggatatcgcc gatacgtggg
    13021 ccaaaatcga taaacggcgt cacgtcttgc agcttttcca gccattccac taacaattcg
    13081 ccgcagggcg aggccagttg ataaccggaa aaatagagca ggctgccagg cgcaacggtt
    13141 aatcgcgcca gccactggcg attccactga ttttccacac cgctgaatga cataaaagtg
    13201 cgttcgccat ccggctcaac cagcgccaga caccagccgt tatcgccttc ggcgttatcg
    13261 atcaggctga ttaacccctc tttcgccatc cggttgcgaa taatctccgc ccacacgccc
    13321 tgaccgagcg gcaaggcatt acccgcttcg atgccgaggc gctttaacgc cacggcaata
    13381 ttcagtgcgc agccgccaac gttaacgctc tgctgcttca gttcgatatc gcacccacgc
    13441 caggggaggg cataagcgtc ggcgatcaca tcaatgaccg ccgcgccgac gaccatcacc
    13501 ggctgacgcg aggttaattc aggcagcagc gtgtgtaatc gagcgccgct cataccgcct
    13561 cccgttgttg acgatatttc gccagcgctg tggcatagcg gttgaaatca agctgattta
    13621 ccgcatccag ttccgccttt aatgcaggat cgatagcgtt aacgccatgc aacgcgccgc
    13681 aaatcgccgt cgccatagcg ccgatggtgt ctgtgtcgcc gccaaggtta gcgcacagga
    13741 cggcgcagcg attcgggtcg gtttgtgcca gttcaaccag cgcaatggcg cacggaacgg
    13801 actcaatagt gctggtacct gcgccaacga cctggtaaag ctgttcgctg gcggattcgg
    13861 tgccgtcggc attgcgcaca attttcagcg caatctccag acgtgctgcc agtgaggcgc
    13921 tgaacgtggt gatgcgtttt tgctgtgcat gtcgcgcaat tgaaggcagt gaatctacaa
    13981 tcgctgacca gctttctccg tcaatggcac gagaaatcgc ccatgcgatg actaccgcgc
    14041 ctgcaaccgc cagatcggat ttatgggtcg ggctggacgc cagcgccaca tcatcaataa
    14101 aggaatcaac atcgtgcgcc ggaagcaaac aacctaatgg cgagacgcgc atcgctgcgc
    14161 cgttggtcac gccgttgttt tccagttcag caatgggttt accgtcgcga atggcgttaa
    14221 gcgcaatctt cgaggtcgga ccaagtacgt ttttgttaaa ggcgtcgaaa cgcagcgccc
    14281 agtcgagaat attacgccca atcagattcg gatcgatctt gccttcacgt tccagtaacg
    14341 catccgccag acacagcgcc atcgaggtat cgtcggtgaa ttcggcgcgg ttaaaataac
    14401 aggccgcgtt attctccttt ggtccaggaa gaaaacggtc aatccagcca aagtgtgctt
    14461 taacgcggct gcgtggccaa agctcggagg gcatccccat cgcatcccct aacgcctgcc
    14521 cataaagagc accgagaata cgttctgttt tcatttaact tccccttgtg tcaacgcaat
    14581 atcgcgatca tcgaccttga tagccgtaat ttcgttgtcg gattcgcgga aaaaaatcat
    14641 gaacagcacg gcgataatgg caatcatcac cgcgccaaaa gtccacatcc cggaccagtt
    14701 gaaagtcagt ccgtttaccg gttcctgata agcgaacatc ttttccatca tcacaccgcc
    14761 aagacgatag ccgagcaaac tgccgaagcc ctggcagcag agcgtgatca gcccctgcgc
    14821 ggcggtacgc atatgcacgg gggctttttt atcgacatag atgtaagcgg taacgtagta
    14881 aaaatcgtaa cttacgccgt gaagcaaaat accgaggaac agtaacgcgt aggtgaaata
    14941 ttcatccgca ctaccgtaaa taaagaagcc atagcggatc gcagcggtga ccagaccaag
    15001 caataatacc tttttgatac caaagcgttt agtgaaaaac ggtaatgcca gcataaagaa
    15061 tatttcagag aactggccga gcgtcatcca gccggtggcg tttttcatgc caacttcggt
    15121 cagataaccg ttggcaaaga tgtaatagaa cgccagtggc atcgcaaaca ggaatgaaca
    15181 gaagaaaaag acgaggaagt ttttatcgcg cagcaggatc agcgcatcca ggccgagcat
    15241 gactttaatg tccattttgc cggtgctttt tggcggcgtg tcgggcagga aaaacgcaaa
    15301 cacaccgagc agagcagaac ttccggcggt aatcagcagc gggatgttag tcggtgagat
    15361 atcggcatac cccagtattt gcggcaagaa accacatgcc agaccggagg caatccagcc
    15421 gatagtgccc atcacacgaa tgcgcgggaa atcacgctca acatccggca cgttggcaaa
    15481 agcgatgctg ttagtcagcg caatggtcgg catataggtt agcgagtagg ccagcagtaa
    15541 cgggaagaac ccggcaaaag tggtctgttg cgcagcgaaa tacatcagca cagcaccagc
    15601 gaacatcaat accgccagca ctttttgcgc cgagaaaaag cggtcagtga tggagccaac
    15661 cagaattggc gacaggatcg ccgcaatggc ggtacaggca tacgaccagc caatttctcc
    15721 ggcactaaaa ccgcttttac ttaaccacaa ccacaatggc acaaaccacg cgccccagat
    15781 aaaccattca acaaacatca tgaacgacag ctttgctgtt gttttcatct tttaattcct
    15841 tcatgacagg taatgtaagg tacgcgatga caataccact taataatacc ttttaaatac
    15901 ctttacggtg aaaacttgac cgcgctaaca ttttttctga tgaatcgagc caacagaaaa
    15961 cgctgaaaaa acatccaaaa gatggaaaaa ctcgcccgta acgaccagga ttatgattgc
    16021 ccgcaaaagg cgggccagga caatccatag ccgatatcca atcggaattt acgggagcat
    16081 agtaatgaca gatattgcgc agttgcttgg caaagacgcc gacaaccttt tacagcaccg
    16141 ttgtatgaca attccttctg accagcttta tctccccgga catgactacg tagaccgcgt
    16201 aatgattgac aataatcgcc cgccagcggt gttacgtaat atgcagacgt tgtacaacac
    16261 cgggcgtctg gctggcacag gatatctttc tattctgccg gttgaccagg gcgttgagca
    16321 ctctgccgga gcttcatttg ctgctaaccc gctctacttt gacccgaaaa acattgttga
    16381 actggcgatc gaagcgggct gtaactgtgt ggcatcaact tacggcgtgt tggcgtcggt
    16441 atcgcggcgc tatgcgcatc gcattccatt tctcgtcaaa cttaatcaca acgagacgct
    16501 aagttacccg aatacctacg atcaaacgct gtatgccagc gtggagcagg ctttcaacat
    16561 gggcgcggtg gcggttggtg cgactatcta ttttggctcg gaagagtcac gtcgccagat
    16621 tgaagaaatt tctgcggctt ttgaacgtgc gcatgagctg ggcatggtga ccgtgctgtg
    16681 ggcctatttg cgtaactccg cctttaagaa agatggcgtt gattaccatg tttccgccga
    16741 cctgaccggt caggcaaacc atctggcggc aaccatcggt gcagatatcg tcaaacaaaa
    16801 aatggcggaa aataacggcg gctataaagc aattaattac ggttacaccg acgatcgtgt
    16861 ttacagcaaa ttgaccagcg aaaacccgat tgatctggtg cgttatcagt tagctaactg
    16921 ctatatgggt cgggctgggt tgataaactc cggcggtgct gcgggcggtg aaactgacct
    16981 cagcgatgca gtgcgtactg cggttatcaa caaacgcgct ggcggaatgg ggctgattct
    17041 tggacgtaag gcgttcaaga aatcgatggc tgacggcgtg aaactgatta atgccgtgca
    17101 ggacgtttat ctcgatagca aaattactat tgcctgatac gcctcatacg aacagcacca
    17161 ccccgtagga cggataaggc gtttacactg catccggcaa cctatgcctg atgcgacgcg
    17221 gaagcgtctt atcatgccta catagcactg ccacgtatgt ttacaccgca tccggcataa
    17281 aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc ctttcactcc
    17341 tcgaacaatt
//
LOCUS       DAZGXZ010000105        17294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000105 DAZGXZ010000000
VERSION     DAZGXZ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.105"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            183..785
                     /gene="ais"
                     /locus_tag="ACHKJF_003728"
     CDS             183..785
                     /gene="ais"
                     /locus_tag="ACHKJF_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP0951010.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLVRLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(824..1249)
                     /gene="nudI"
                     /locus_tag="ACHKJF_003729"
     CDS             complement(824..1249)
                     /gene="nudI"
                     /locus_tag="ACHKJF_003729"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP0951011.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            1528..2070
                     /gene="yfaZ"
                     /locus_tag="ACHKJF_003730"
     CDS             1528..2070
                     /gene="yfaZ"
                     /locus_tag="ACHKJF_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP0951012.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            2170..3372
                     /gene="yfaY"
                     /locus_tag="ACHKJF_003731"
     CDS             2170..3372
                     /gene="yfaY"
                     /locus_tag="ACHKJF_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP0951013.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            3436..3897
                     /locus_tag="ACHKJF_003732"
                     /pseudo
     CDS             3436..3897
                     /locus_tag="ACHKJF_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(3938..4840)
                     /gene="rpnE"
                     /locus_tag="ACHKJF_003733"
     CDS             complement(3938..4840)
                     /gene="rpnE"
                     /locus_tag="ACHKJF_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP0951014.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLGRQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(5034..6224)
                     /gene="glpC"
                     /locus_tag="ACHKJF_003734"
     CDS             complement(5034..6224)
                     /gene="glpC"
                     /locus_tag="ACHKJF_003734"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP0951015.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(6221..7480)
                     /gene="glpB"
                     /locus_tag="ACHKJF_003735"
     CDS             complement(6221..7480)
                     /gene="glpB"
                     /locus_tag="ACHKJF_003735"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP0951016.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(7470..9098)
                     /gene="glpA"
                     /locus_tag="ACHKJF_003736"
     CDS             complement(7470..9098)
                     /gene="glpA"
                     /locus_tag="ACHKJF_003736"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP0951017.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            9371..10729
                     /gene="glpT"
                     /locus_tag="ACHKJF_003737"
     CDS             9371..10729
                     /gene="glpT"
                     /locus_tag="ACHKJF_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP0951018.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            10734..11810
                     /gene="glpQ"
                     /locus_tag="ACHKJF_003738"
     CDS             10734..11810
                     /gene="glpQ"
                     /locus_tag="ACHKJF_003738"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0951019.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(11852..12091)
                     /locus_tag="ACHKJF_003739"
                     /pseudo
     CDS             complement(11852..12091)
                     /locus_tag="ACHKJF_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            12089..12193
                     /locus_tag="ACHKJF_003740"
                     /pseudo
     CDS             12089..12193
                     /locus_tag="ACHKJF_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12273..12923
                     /gene="inaA"
                     /locus_tag="ACHKJF_003741"
     CDS             12273..12923
                     /gene="inaA"
                     /locus_tag="ACHKJF_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP0951020.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(12977..13231)
                     /gene="yfaE"
                     /locus_tag="ACHKJF_003742"
     CDS             complement(12977..13231)
                     /gene="yfaE"
                     /locus_tag="ACHKJF_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP0951021.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(13231..14361)
                     /gene="nrdB"
                     /locus_tag="ACHKJF_003743"
     CDS             complement(13231..14361)
                     /gene="nrdB"
                     /locus_tag="ACHKJF_003743"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0951022.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(14450..16735)
                     /gene="nrdA"
                     /locus_tag="ACHKJF_003744"
     CDS             complement(14450..16735)
                     /gene="nrdA"
                     /locus_tag="ACHKJF_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0951023.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            16863..17006
                     /gene="ypaB"
                     /locus_tag="ACHKJF_003745"
     CDS             16863..17006
                     /gene="ypaB"
                     /locus_tag="ACHKJF_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP0951024.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         4095 a         4203 c         4600 g         4396 t
ORIGIN      
        1 tagacgtaaa tattgaaatt tttatatttt ttcttattta ggctttgcat ttggcaaaat
       61 tttgaggcat tttgccgaca tcgtaggatt tttaatatta caccaactgc taattatcgc
      121 cagaaatgtc gctcaatttt acggtaattg tctggttgcg cttgtctata ggtggagttt
      181 acgtgttagc tttttgccgc tcatcattga agtcaaaaaa atatttcatc attttactgg
      241 cgctcgctgc aattgccgga ctgggtactc atgccgcctg gagtagcaat ggtttgccac
      301 gtatcgacaa taaaacattg gtcagactgg cacagcagca cccggttgtc gttttgtttc
      361 gccatgctga acgttgcgat cgttcaacca atcaatgctt gtcagataaa acaggtatta
      421 cggttaaagg tacccaggat gcccgtgaac tgggcaacgc ttttagcgct gacatccctg
      481 atttcgatct ttattccagt aataccgtcc ggaccattca gtcggcgacc tggttttccg
      541 cgggtaaaaa gctgacggta gataaacgac ttcttcagtg cggtaatgag atttatagtg
      601 caattaagga cttacaaagc aaagcgcctg ataaaaatat cgttattttc acccataatc
      661 attgcctgac atatattgct aaaaataagc gtgatgcgac atttaaacct gattatctgg
      721 atggtttagt catgcatgtg gaaaaaggca aagtttatct ggatggggaa ttcgttaacc
      781 actaaatttt caatctgaca gccagtgatg gctgtcatca ttgttaaaga agacctttca
      841 aacgtaacgt ttttcgggtg gcaacattta aatcataatg caccagatct tcaggtttta
      901 cccacgcgta gtcctgaaac tcttcgttta ttttcacttc tcggttggca gaaacgcagt
      961 caaaaatcag gtaaatcata taaatctctt ccttgcgacc atctgcatac gtcttggtgc
     1021 gaatatcatc gctgaaggtc cacggcgtga tttctgtcaa aagcagctgt tctcccagtt
     1081 cttcgcgaat ttcgcggcgt agtgcctctt caattcgttc gccagactcc acgccgccac
     1141 ccgaaagcgc ccattgaccg gggaaaacgc cgcgatcgtc ggccatttta cacagcaaat
     1201 aagcaccatc attttgaatc aaagggcata caatagtccg ttgtcgcata ggggtattcc
     1261 ttataaataa aaagaaacca tgagaatacg gtaactcaga aatatgtgaa agtgacagta
     1321 atctcttttg caatgataac tgcacttatt attatttttg tgtgtatcgg taaagtgtgt
     1381 ttattttttc ggcgtttaat tatttaagaa gtatttagtt acaataagtg actagatccc
     1441 ttttttcgct cattgaatcc tctgtgccag gcagtacaat cccgcagctt tgggaatttt
     1501 gattaattgc ttgtgggaaa ggggataatg aaaaaaattg cgctagcagg tctggccgga
     1561 atgctgttgg tttctgcatc ggtcaatgca atgagcatca gcggccaggc gggtaaagaa
     1621 tacaccaata ttggtgtcgg ttttgggact gaatcgacgg gcctggcttt aagcggtaac
     1681 tggacacata acgacgacga cggtgacgtc gcgggcgtgg ggctggggtt gaatctgcct
     1741 ctcgggccgt taatggcgac cgttggcgga aaaggcgttt acaccaaccc gaattacggc
     1801 gatgaaggtt atgccgcagc ggtaggaggt ggtttgcagt ggaaaattgg caacagcttc
     1861 cgtttgtttg gcgagtatta ctactctccg gattcgctct ccagcggtat tcaaagttat
     1921 gaggaagcga atgctggcgc gcgttacacc attatgcgtc cagtcagtat tgaggcgggt
     1981 tatcgctacc tgaatctgtc gggtaaagac ggtaaccgcg acaacgccgt ggctgacggc
     2041 ccgtatgttg gggttaacgc cagtttctga tcccttccgg cgcggcattc tgtcgcgcca
     2101 ttcagtttct tactacgcct tatctccctt tgcgttatag tgattcgata acaaaagcag
     2161 gagataacca tgttaaaagt ggaaatgcta tccaccgggg atgaagtgtt acacgggcaa
     2221 atcgttgaca ctaacgctgc ctggctggcc gattttttct ttcatcaggg gctgccatta
     2281 tctcgccgca atacggtggg ggataacctt gatgacttag tcaccattct tcgcgaacgt
     2341 agtcagcacg ccgatgtgct gatcgttaac ggcgggctgg gaccgaccag cgatgattta
     2401 agcgcactcg ccgctgcgac agcaaaaggt gaaggcctgg tgctgcatga agcctggctc
     2461 aaagagatgg aacgctattt ccacgaacgt ggacgagtaa tggcaccgag caaccgtaaa
     2521 caagcggagc tgcctgccag tgctgaattt atcaataacc cggtaggcac cgcctgtggt
     2581 tttgccgtgc agcttaatcg ttgcctgatg ttctttactc ccggcgtacc gtcagaattt
     2641 aaggtgatgg tcgagcacga aatcctgccg cgtctgcgcg agcgtttttc tttaccacag
     2701 ccgccggttt gtctgcgttt aactaccttt ggtcgttcgg aaagcgatct ggcacaaagc
     2761 ctggacactc tacaactgcc gccgggcgta acaatgggct atcgctcttc aatgccgatt
     2821 atcgaactga aactcaccgg accggcaagc gagcaacagg cgatggaaaa actgtggctg
     2881 gacgttaaac gtgttgccgg acagagcgtg attttcgaag gcaccgaagg actgcccgcg
     2941 cagatcagtc gcgaattgca aaaccgccag ttcagcctga cgttgagcga gcaattcacc
     3001 ggtggtttat tggctttgca actttctcgc gcaggtgctc cattgctggc gtgtgaagtg
     3061 gttccttcac aggaagaaac cctggcgcaa actgcgcact ggattacaga acggcgggcc
     3121 aaccattttg ccgggctggc actggctgtt tcgggtttcg agaacgaaca tctcaacttt
     3181 gcgctagcca caccagacgg cactttcgct ctgcgtgtgc gtttcagcac tacgcgctac
     3241 agcctggcta tccgtcagga agtgtgcgca atgatggcac tgaatatgct gcgccgttgg
     3301 ttaaacggcc aggatatcgc cagtgagcat ggctggattg aggttattga gtccatgacc
     3361 ttatctgtct gattccgcca ctctgtgccg gtttactgca ccggcacatt ccccctccgg
     3421 caaaacttgt ccaaccctcc ctacggtgag cgtggtgttg gggccagtgt ggcacgggct
     3481 gcgcgctggg gacgcattga gaattacatg gcgcaagtta acgattcact ttgtctgttg
     3541 gtgcaggtgg aaagtaaaac ggcactggat aacctggacg agatcctcga cgtcgaaggg
     3601 attgatggcg tgtttattgg accagcggat ctttctgcgt cgttgggcta cccggataac
     3661 gccgggcacc cggaagtgca gcgaattatt gaaaccagca ttcggcggat ccgtgctgcg
     3721 ggtaaagcgg ctggttttct ggctgtggct cctgatatgg cgcagcaatg cctggcgtgg
     3781 ggagcgaact ttgtcgctgt tggcgttgac acgatgctct acagcgatgc cctggatcaa
     3841 cgactggcga tgtttaaatc aggcaaaaat gggccacgca taaaaggtag ttattgatat
     3901 caaaggccca tggggatcgg ctgtgggcct gtgttaatta gtggttattc gctgccagat
     3961 cggcttcgct tagctgggtg gccgcgagca cctggtcacg atcaatgccc tgttccagca
     4021 tgcgcaaagc aattttaatg gcttgttcat gcataccttc ctgccagcca atttgatgcc
     4081 cttcctgccg tagccgttca gcaatagtca ttaatctctc cttttgtagt ggtgaacgtt
     4141 cggcaatctc ctcaataaaa cgggtgaaac gggaggtatc gccgcattgc agcagataat
     4201 taaacagtgt ttgtagctgg ctgtcattag tgaagcctct aaccaaaagc gtggtgatcc
     4261 tgtcaaccat gccgattaaa tcgcggtcgc gaatatgctt ttgaatcagt tccagcagag
     4321 ctatacgccg atgttgcatg atctcatcgt caggtacgat ggtgatatcc accaacggaa
     4381 aagcttcggt gtacaactgc cggccaagtt gcggatcgtc aaactcatcc agccagttaa
     4441 gtgagtacgg gtagggcgat gtttcgccat gataaaacag caatggcacc accagcggaa
     4501 ctctgtcata gcctttatcc aggtgacgct gcatggcggc agtggcatag cgcattagcc
     4561 gaaaagccat attcttttct gcagagcttt gatgttcaat cacgcagtag atatagccgt
     4621 caccgtcgct ggtttccacg gaccacaaaa catccgagta gtaagcgcgt aaactttttt
     4681 caataaaact agtgggttcc aggcgtaagg tttgcaggtt gcaaagcttg cgcagtggtt
     4741 ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg tgtgaacata aaggttttaa
     4801 acaccgcatc atgcggggag gatgttgttg attcggtcat ggttgtccgt cacctgatat
     4861 ttatgatgac gcgacattac gtctatcccg ggcctttgac atctgtcagt ttcagccttt
     4921 gcgagacatt ttcagaggaa ttttcaaagc gttgcaacgc atacatggtg cgtggaatcg
     4981 cgctcgcaaa aatgcccgat aagcaatgtg cttaccgggc atcaaaaggt cggttaagcc
     5041 agcgcctggg ccagtagcgt aatcggatgt tcgcagcgaa ggctcgtgga catctcaatc
     5101 tgccatttgc aggtttcgca gtcggtgatg accagatccg cgccgctctc ttctatctgg
     5161 cggaacagtg gcgcaccgat ggcttgtgag gtggggtagt tctctttttt gaaaccgtaa
     5221 gtacccgcaa taccgcagca ctgggaatcc agcaccgtta actcaagccc cgggatttta
     5281 cgcaacagct ccagggtgta gagcgtccag cccatttttt ccatatggca cggagtgtga
     5341 taaaccactt tcagcggcag cggtttcagc ggtaacgttt tgccttcgtc cagcttgcgc
     5401 cacagccagc gggttgccag ttcgatatga tcgcgcaagc ctttgttgtc gacattcagc
     5461 acttccgggt attcgtcgcg cagggcaaat gtacaggttg aggaggtggc aatcaccgga
     5521 atgcctttta ctcccacagc ttcgcggatc gactctacat tcgtaattgc ctgtttgcgt
     5581 gctttatcgg taaagccgtt ggcgattagc ggtacgccgc agcatttttc tttgctgagc
     5641 agttgtacac cggtacccat tgcgttgagc actttaatta aatctttacc taactgcgga
     5701 tggttgtagt taacgaagca gccgtgaaag aaagcgacct ggtctttata ttgtgcttgc
     5761 tgagccgcta tgctgcgata ccagcgacgg aacgtgccga aggagtattt cggtagcgtg
     5821 cggcgatgat cgatttttaa cgccgcatca agcagctgcc gcaccggttt cagcgaggta
     5881 gcggtgttga cgattggtgc gaacggcgtg gaaacgctac ccatcaggtc ggtatgactc
     5941 aacacaaaat tacgcagcga cgggcgcgtg gtgtcatatt ttgcccgcgc gcgctggata
     6001 atatcgccaa tcttcacatc ggacgggcag gcgacttcac aacgtttgca gttgatgcaa
     6061 tatttcagcg cctcgtcata cagtgcgcca tctttcaaac gcagacgctc gccatccggc
     6121 ccggcttgtt ttggccctgg ataaccggga ttcacccggc tcaccgggca ggcggtggtg
     6181 cagacggtgc acttaatgca gttttcgaag ctggtgtcgt tcattgttgg cctcctgcgc
     6241 gttgggcaat ctgttgagcg gcatgtaaag cactgacggc acaaacaccg ccgccgcatc
     6301 cctgggcgat gggatcaaat ccgcccagca ccgaaccgat ggcaaacagg ttctcaatgg
     6361 tttgccctgc ctgcgacggg cgtagcgtct catcagtggt tacaccgaac tgctgccacg
     6421 gttgcggcgc aaaaaaatcg cccttatacc attcaccccg cgtggcggtt tgtagcacat
     6481 caaggccgag aatcggctcg cgaatgccgt tacgttccgc taccagtccg ccactaaaga
     6541 aactgccgct ggcgagaacc gcgaaacgtg gacgtagcgg aatatcggcg tgattgcggg
     6601 tccagatttc attcactacg ccatttttac aggtcacttt tttcacttca tcgcccggca
     6661 tccacacgcc accctggcgc acaaactggc gctgtaactg gttttgcaga cgaatgccca
     6721 gcacggaagg cggcagcgtt ggcaaaagca tcagtgaaca aggtagtttt tcattcaacc
     6781 aacgccacag tttgtcatcg gccaggccga agcaggcggg catcaggatc atttcgcagg
     6841 tattggcgac aggaataagc gcatcaagta acagcggcca gttttcttca ttatcaagga
     6901 aacgggcgat attcaccgcg cgaaattcgg tggcgttatt gcgcaggaca tccagttccg
     6961 gcagctctat ttctgcggtt tcaaccgaaa gatccagttc gcgcaacgaa gccgccgcaa
     7021 gatgcgcctg gaaatccatc agcccgctta tccccactac acatatcttc ttcgcgggta
     7081 gcggccagac gggaacttct ggcgaactta accaggttgc acgcaaagtg ccgagcgggg
     7141 taacgcgctg atgggccagt tccacgctgc cctgcaattg cgcaccgctt tcagcgatca
     7201 atgcctgcgc ctggcaagcg agatcgagca cgcgttgtgg ttcgagaaga ctgtaaggat
     7261 gggctggtgc ctgctgacgc aaagattcca gtccactgtg aatgtctgtc accggttgac
     7321 catcaggcag atggctcagc aaatccagcg aaccggatga gaaatgcagc gcgctttgac
     7381 cacgcgtgac aatggcacag cgcaggccgt gtttttgcag ttgcaggcca cagagtaatc
     7441 cggcgaggcc gccgcccata atgacagtat caaagcgcat ctttctgctc cttctccaga
     7501 ccacacaatc cctgataaac ccagcgggta aattcgcttt cgcgcagtgc atctccccag
     7561 gcgatgggtt gcacgccttt ccagcgttcg ttaaggaagg tggaaagttg ctcgatagat
     7621 tgcgcggacg tcgtgacgtt aaaacgttgc agcagtccgg cagcgcggca ggcgcagagt
     7681 tcgccctggc aggtacccat ccccacacgg gtacgacggc gtaaatccag caggctatta
     7741 acgtttaaat tttctaccgc gtactgaact tcacccgcag ttaccgcttc gcactcacat
     7801 accaggctac gatgcagacg gccttcgctc agccaggcag gcgtgcgatc gccatgacga
     7861 taaaccgcag aaccgcgcag cggggcaggc agggagatga ctttacgcaa ggtaacttca
     7921 gcgggttctt gtgaaccagg cagtgccaga tcggcagtcg tacaggggcg cgtgttgccc
     7981 agtttgcggc ataccgcgtc ggtagcccat tcagccatca gccgataggt catcagtttg
     8041 ccaccggtga tggtgataaa tccgtccaga ccatcgcgtt cagcatggtc gagcagcacg
     8101 atgccacggc tgacgttacg tccgctcggg tcgtcatcgc tggcaaccag cgggcgcacg
     8161 ccagaatagg cccgcaaaat gcgcgttttc gccatcacgg gggccagttt ttccccttca
     8221 cgcagcagaa tatcaacctc ttctgccgtc actcgattat cgtcaatctc gttgtaatca
     8281 atacgtaaag aggtggtgcc aatcagcgaa atggtatcgc caggcaccag aatatcggcg
     8341 tcggaaggtt tacggcagcg gttgatcaca tgctggttaa tgcggtgatc catgatcagc
     8401 agcgatcctt tcgccgggaa catgcgaatg cgcagatcgg catattcggc aatgtgttgc
     8461 ccccagatcc cagcggcatt aaccacgaca ggtgcatgaa gggcctgagt ttcgccggtg
     8521 agatgattac gtacacgaac accgcacacc gtcgcgcctt cacgaatcag ccccgtgact
     8581 tcatgagcgg taaggataac ggcaccgtgt tctttggcat ccagcatgtt tgctgcggtc
     8641 agacgaaatg gatcaacggt gccatccgga actttcaccg cgccaatcag tgccgggtta
     8701 acggcaggtt cgataatgcg cgcttgctgc gggtctatag cttctgcgct gatccctgct
     8761 tcttcgcagg cgcgaataaa agtggcctgg aatgagaggt catcttccgg cagggtgata
     8821 aacaggccgt tggttggttc aacgcagtga cgtgcaatgc gtttcaggat ctggttttca
     8881 ctaatgcatt cgcgggccga ttccgcatcg gttaccgcat agcgcgcacc gctgtgcagc
     8941 aggccgtggt tacgcccggt ggcaccggtt gcgatgtcgt ggcgctcaac caaaatcacg
     9001 cgcagcccgc gcagggcaca gtcgcgggca atcccggctc ccgttgcgcc gccaccaatg
     9061 ataatcacgt cacttgattg cgagtcgcga gttttcattg ttatccctct gaagttcgtt
     9121 ttttaccatt tagccatagt aaaaacatga attgtttgat ttcgcgcata ttcgctcata
     9181 attcgaaagt gaaacgtgat ttcatgcgtc attctgaaca ttttgtaaat cttatttaat
     9241 aatgtgtgcg gcaattcaca tttaatttct gaatgttttc ttaacatcgc ggcaactaaa
     9301 gaaacggcag gttctcccac tgaatcagtc tgttaatcat aaataagacc acgggccacg
     9361 gaggctttca atgttgagta tttttaaacc agcgccacac aaagcgcgct tacctgctgc
     9421 ggagatcgat ccgacttatc gtcgattgcg ctggcaaatt ttcctgggga tattctttgg
     9481 ctatgcggct tactatttgg ttcgtaagaa ctttgcgctt gctatgcctt atctggtcga
     9541 gcagggattc tctcgcggtg atttaggttt cgcgctttcg gggatctcga tcgcttatgg
     9601 attttcgaaa ttcataatgg gttcggtatc ggatcgctcg aatccgcgcg ttttcctgcc
     9661 cgcaggtttg attctggcgg cggcagtgat gttgtttatg ggctttgtgc catgggcgac
     9721 gtcgagcatt gcggtgatgt ttgtactgtt gttcctctgc ggttggttcc aggggatggg
     9781 gtggccgccg tgtggtcgta ctatggtgca ctggtggtcg cagaaagaac gtggcggcat
     9841 tgtgtcagtg tggaactgtg cgcacaacgt cggtggtggt attccgccgc tgctgttcct
     9901 gctggggatg gcctggttca atgactggca tgcggcgctc tatatgcctg ctttctgcgc
     9961 cattctggtg gcattattcg cctttgcgat gatgcgcgat accccgcaat cctgtggctt
    10021 gccgccgatc gaagagtaca aaaatgatta tccggacgac tataacgaaa aagcggaaca
    10081 ggagctgacg gcgaagcaaa tcttcatgca gtacgtactg ccgaacaaac tgctgtggta
    10141 tatcgccatc gccaacgtgt tcgtttatct gctgcgttac ggcatcctcg actggtcacc
    10201 gacttacctg aaagaggtta aacatttcgc gctggataaa tcttcctggg cctacttctt
    10261 ctatgagtat gcgggtattc cgggcaccct gctgtgcggc tggatgtcgg ataaagtctt
    10321 ccgtggcaac cgtggggcaa ccggcgtttt ctttatgaca ctggtgacca tcgcgactat
    10381 cgtttactgg atgaacccgg caggtaaccc aaccgtcgat atgatttgta tgattgttat
    10441 cggcttcctg atctacggtc ctgtgatgct gatcggtctg catgcgctgg aactggcacc
    10501 gaaaaaagcg gcaggtacgg cagcgggctt taccgggctg tttggttacc tgggcggttc
    10561 ggtggcggcg agcgcgattg ttggctacac cgtggacttc ttcggctggg atggcggctt
    10621 tatggtaatg attggcggca gcattctggc ggttatcttg ttgattgttg tgatgattgg
    10681 cgaaaaacgt cgccatgaac aattactgca aaaacgcaac ggaggctaat ggcatgaaat
    10741 tgaagctgaa aaaccttagc atggcgatca tgatgagcac tatagtcatg ggaagcagtg
    10801 caatggcggc ggacagcaac gaaaaaatag tcatcgccca tcgcggtgcc agtggatatt
    10861 tgccggagca tacgctgcca gcaaaagcga tggcgtatgc gcagggagcg gattatctgg
    10921 aacaggattt ggtgatgacc aaagacgacc atctggttgt tctgcatgac cattatctcg
    10981 atcgtgttac tgatgttgcc gatcgtttcc cggatcgggc gcgcaaagac ggtcgttact
    11041 acgcgataga tttcacgctg gatgaaatta agtcgctgaa atttaccgaa ggtttcgata
    11101 ttgaaaacgg taaaaaagta cagacttatc cggggcgttt cccaatgggt aagtccgact
    11161 tccgggtgca cacctttgaa gaagagattg aatttgttca ggggttaaat cactctaccg
    11221 ggaaaaatat cggtatctat ccagaaatta aagcgccgtg gttccatcat caggaaggga
    11281 aggatattgc ggcaaaaacg ctggaagtgc tgaagaaata tggttacacc ggtaaggacg
    11341 acaaagttta tttgcaatgt tttgatgctg atgagctgaa gcgtattaag aatgagctgg
    11401 aacccaaaat gggcatggat ctcaatctgg tacagctgat tgcctatacc gactggaatg
    11461 aaacgcagca gaaacagccg gatggaagct gggttaatta caactacgac tggatgttta
    11521 agccgggtgc tatgaaacag gtggcggaat atgccgacgg tatcgggccg gattaccata
    11581 tgttgattga ggaaacatcg cagccgggta atatcaaact cactggcatg gtgcaagatg
    11641 ctcagcagaa taaactggta gtgcatcctt ataccgtgcg gtcagataaa ctgcctgaat
    11701 acactactga tgtgaatcag ttatatgatg ctctgtataa caaagcgggt gtaaatgggc
    11761 tgtttactga tttccctgat aaggcagtaa aattccttaa taaagagtaa tgctactgca
    11821 ggatggcgtt catgcgccat cctgtctcac atcaaatttt tccattcacc acgagttgtt
    11881 ccgtacacgt ttgtagtaaa aacagtggac tgccttctac cggcttttct ttagccaaca
    11941 acgaaatctg tcgatagaag gttggttcgt gaggaacgga acacaattca cctgcaatgc
    12001 ttttcagcgt taactctggt tgtaaggcga tgcctaatcc ctgtcgaata aacttcatcg
    12061 ctgtatccga ctgattaaat tcacatttta tctactgggc gcactattag tggtagtggc
    12121 tggtggaatc tatgcgctgc aaatgaaggg taaaagcggt gtcacgctag tagtggcaaa
    12181 agaaattcat taatacgaca cgtttcatta agattttcct caggtaacca cctatagtca
    12241 ttgtcgttaa taaaaatgac aggcgatagg gtatggcagt ttcagcaaag tacgacgaat
    12301 ttaatcactg gtgggcaaca gagggcgact gggttgaaga gcctaactat cgccgtaatg
    12361 gtatgagcgg ggtgcaatgc gtcgagcgca acggcaaaaa gctgtatgta aagcgcatga
    12421 cgcatcatct gtttcattcc gtacgttatc cgttcggccg accaacgatt gtccgtgagg
    12481 ttgcggtaat taaagaactg gaacgggccg gtgtcatcgt gccgaagatc gtttttggtg
    12541 aagcggtgaa aattgagggt gaatggcgag cgttgctggt gactgaagat atggcggggt
    12601 tcatcagcat tgctgactgg tatgcccagc atgcagtatc gccttattct gacgaagtac
    12661 ggcaagccat gttgaaagca gtggcgctgg cgtttaagaa aatgcatagc attaatcgtc
    12721 agcatggctg ttgttatgtt cgccatatct atgtgaaaac agaaggtaat gcagaagctg
    12781 gttttcttga cctggagaaa agtcgtcgcc gcttgcgtcg cgataaagcg attaatcatg
    12841 acttccgcca gctggaaaaa tatctggagc caatccccaa agcggactgg gagcaggtga
    12901 aagcgtatta ctacgccatg taatcgttgt aggccagata agacgcataa gcgttgtatc
    12961 tggcactaca accaattcac atctcgattt caatatcgcc ttttgcccgg caacaacagg
    13021 gcaaaatttc ccccggctga ataaaggcta acggttcggc aatccagtca acctgacctg
    13081 caaccagacg ggtgcgacag gagccgcagt aaccttcgcg acactgatac tcaaccgcca
    13141 cattgtggga ttccagcgcc gccagaaggg aagggtgttc atcctggcac agcagttgtg
    13201 tgccagtgat gcgcagggta acgcgggcca tcagagttgg aagttactca aatcgtcggt
    13261 gtccacttcc gcgtcaatct gcccgaccag ataagaactg acttccactt cctgcggagc
    13321 aacctgcacg ttatcagaca ccagccaggt gttgatccat gggatcgggt tggagcgcgt
    13381 ctggaacggc agatccaaac cgactgcctg catacggata ttggtgatgt attcaacgta
    13441 ctggcagaga atgtctttat tcagaccaat catcgaaccg tcgcggaaca gataatccgc
    13501 ccagtctttc tcctgttgag ctgcctgaac aaacaggtca tagcactcct gcttacactc
    13561 ttcggcaatt tccgccatct caggatcgtc cgcgccgctg cgcagcagat tcagcatatg
    13621 ctgggtgccg gtcaggtgca gggcttcgtc gcgggcaatc aggcgaataa ttttggcgtt
    13681 gccttccatc aattcgcgtt ctgcaaatgc gaaggaacaa gcaaagctga cgtagaaacg
    13741 aatcgcttcc agcgcgttaa cgctcatcag gcagagatac agttttttct tcagctcgcg
    13801 caggctaacg gtcacagttt taccgttaac ggtgtgggta ccttcgccca gcagatgcca
    13861 gtagctggtc atttcgatca gctcatcgta atagctggag atcccttccg cacgtttctg
    13921 gatctgctcg ttggtgacga tatcgtcaaa cacaacagac ggatcgttaa cgatattacg
    13981 aatgatatga gtataggaac gggaatgaat cgtttctgag aacgcccagg tttcgaccca
    14041 ggtctccagt tccgggatcg agatcagcgg cagcagtgcc acgttcgggc tacggccctg
    14101 aatggaatcc agcagcgtct gatatttcag gttgctgata aagatgtgtt tttcgtgttc
    14161 aggcagtgcc tggtaatcga tacggtcgcg agaaacgtca acttcttccg gacgccagaa
    14221 gaaagagagc tgcttttcga tcagcttttc gaagatgtca tatttttgct gatcgtagcg
    14281 agccacgttg accggctgac caaagaacat cggttctttg agctgatcat ttttcgtctg
    14341 tgaaaaggtg gtatatgcca tgagtgtgtc ctgtggtttt attacccctc accctaaccc
    14401 tctcccgcaa ggggagaggg gacggtttgg tgcaaaggct gggggattat cagatcttac
    14461 atgcgccgct ttcgcagcca tcgtcctgga ttgacggcac cagatcgtcc tgcgcgtctt
    14521 cagcgccgtc acgggtgttc tgataataca gcgttttgac cccgaatttg taggcggtga
    14581 gcaggtcttt cagcaactgc tgcatcggca cttttcctga cgggaaacgt gacggatcgt
    14641 agttggtgtt agcagagatc gactgatcga taaatttctg catgatacct accagttgca
    14701 ggtagccatc gttacccggc atttcccaca gcagctcata ggcgtcgtgc aggtgctcgt
    14761 agtccggcac cacctggcgc aaaataccgt ctttcgacgc tttgatgctg acgtaaccgc
    14821 gcggcggttc aataccgtta gtggcgttag agatctgtga agaagtctct gacggcatca
    14881 gggcagaaag cgtggagtta cgcaggccgt gcgttttgat tgattcacgc aatgcttccc
    14941 agtcgtaatg cagcggctca ttagcgatgg tatccagatc tttcttatag gtatcgatcg
    15001 gcaggatccc tttcgcgtaa gtggtttcgt taaaccacgg gcacgcgcct tgctcttttg
    15061 ccagctcgtt agacgctttc agcaggtaat actgaatcgc ttcgaaggtt ttatgcgtca
    15121 ggttgttggc gctgccgtcg gagtaacgca caccgtgctt cgccaggtag taagcgaagt
    15181 tgatcacacc aatacccagc gtacgacgac ccatcgctcc acgtttggcg gccgggatcg
    15241 ggtaatcctg ataatccagc agcgcgtcaa gtgcacgaac cgccagaatt gccagctctt
    15301 ccagttcatc caggctatta attgcgccca ggttgaaagc agacagcgta cacagcgcga
    15361 tttcaccgtt ctcgtcgttg acgtcgttca gcggtttggt cggcagggct atctccaggc
    15421 acaggttaga ctgacgcact ggcgcgatgg ccggatcaaa cgggctatgg gtattgcagt
    15481 ggtcaacgtt ctgaatatag atacgaccgg tagacgcacg ttcctgcatc atcagcgaga
    15541 acagctcaac ggctttcaca cgctgcttgc ggatgctgtc gtctttctca tatttggtat
    15601 acaggcgttc aaactcttcc tgatcggcga agaatgcgtc gtacagcccc ggtacgtcgg
    15661 acgggctgaa cagggtgata tcttcacctt tcagcaggcg ggtatacatc agtttgttga
    15721 tctgtacccc gtagtccata tggcgcacgc ggttgccttc cacaccacgg ttgtttttca
    15781 acaccagcag gctttccact tccagatgcc acatcgggta gaacagcgtt gccgcaccgc
    15841 cgcgcacgcc gccctgagag caggatttca ccgctgtctg gaaatgtttg tagaacggaa
    15901 tacagccggt atggaacgct tcaccaccgc gaatcgggct acccagcgca cgaatacgcc
    15961 cggcgttgat gccgatcccg gcacgctggg aaacgtattt cacaatcgcg ctggaggtag
    16021 cgttgatgga atccagactg tcaccgcact cgatcagtac gcaggagctg aactgacgag
    16081 tcggggtacg cacgccggac atgattggcg tcggcagcga aattttgaat gtggaaaccg
    16141 cgtcgtaaaa acgcttcaca tattgcaggc gcgtttcacg cgggtagttc gagaacaggc
    16201 acgcggcaac cagaatataa aggaactggg cgctttcgta gatttcgccg gtcacgcggt
    16261 tctgtaccag atatttgcct tccagctgct taacggcagc ataagagaag gtcatatcac
    16321 ggtcgtgatc gataaacgtg tccatctgct tgaactcttc ttccgtgtag tcttccagca
    16381 gatgattatc gtatttgccc atctcgacca ttttcaccac gtggtcgtac agcgcaggcg
    16441 gctcaaactg gccgtaggct tttttacgca ggtggaagat cgccaggcgc gcggcgagat
    16501 actgataatc cggcgcatca cgggagatca ggtctgcggc cgccttgata atggtttcgt
    16561 ggatgtcaga ggtcttgata ccgtcataaa actgaatgtg ggagcgcagc tcgacctggg
    16621 aaatcgaaac gttatgcagt ccttctgccg cccaatccag aacgcgatgg attttgtcga
    16681 gattgatgcg ctctgtgcta ccgtcgcgct ttgtcaccag cagattctga ttcatgtatg
    16741 tcgtacctgt ttttggaatt ctttccgcaa tagtttcatg cagactacct gtagtacggg
    16801 aagctgctta gaacactata tatagggggt atgtttggga ttcactgcaa gatagtgtga
    16861 aaatggccct cttgcaagtg cataactttg tggataactc aggaaggaaa aagtggcttt
    16921 cgcgcacctt aggtcagaca aggtgtccgg gaaagtcaat gggaagagaa aaatttgtta
    16981 aaaataactg ttcggcgatt tcttgaacgg cagaataggt tggataaatt gatatagatg
    17041 gtttattaat ctttttgcta atgattgcaa aaattgatct aagcacgagt catcacataa
    17101 tctgatgtat cagttgtggt caatatataa gcgaatgtaa gcgattgttg atgtcgaaat
    17161 ggttgttttc tattcggtta ttgttaaatg cgaagccatt ttgttaattt accttatatt
    17221 ataagaaaat tattcattac tttaatttag tttaaatata actggtatcc cttaaggtta
    17281 ttaacttgaa ttat
//
LOCUS       DAZGXZ010000106        17165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000106 DAZGXZ010000000
VERSION     DAZGXZ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.106"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..212
                     /locus_tag="ACHKJF_003746"
     CDS             <1..212
                     /locus_tag="ACHKJF_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP0951025.1"
                     /translation="KDKAAGVSAETAQQRIKEFHSEKFFHSLRSTTTFAGRKYTNSDM
                     PSPKKMKLMADTISAVYLDGYEGRQ"
     gene            609..1727
                     /locus_tag="ACHKJF_003747"
     CDS             609..1727
                     /locus_tag="ACHKJF_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951026.1"
                     /translation="MTKLYIYLVLAASLSALSTDMIVPALPELQKAFHADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANDLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKETNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFHYSVVFSLSVLFFIIGNQLSKIEKISNPWVLFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVFLGTTLSISCLILALLSMFVTIIIGNNKK"
     gene            1867..2949
                     /locus_tag="ACHKJF_003748"
     CDS             1867..2949
                     /locus_tag="ACHKJF_003748"
                     /inference="COORDINATES: protein motif:HMM:NF005697.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0951027.1"
                     /translation="MSCSYLGLETHENLKHAIANAIERFGVQLSVARTRVKVDLFEQL
                     EKRLNKIMNGAHTLTFNAVTPCHLAVLPLLASGTLPNYKFNKKPYFILEKTAHATLQI
                     NRGLLQQFGETIRVDFQHKNNIEEAFKYASINNMVPISISDSVGSMGGVAPVEFIVGM
                     ANRFDGYAYIDDAHGMSIYGKNGSGYVMSCLDNKLDDRIIIAGSLSKAFGSHGGFIAC
                     NYPDTINFIKTYGTTYAFGGPPSLPGIAACIAAADLHLDGTVSLRQNDLQQVIKYFDD
                     KFNETEVINKGTTMPIRGLLIGNEEKAITICKKMHDRGFATTVAMYPTVEEGKAILRC
                     AISAIHTRENIDSLHKNFIECINEQG"
     gene            complement(3020..3166)
                     /locus_tag="ACHKJF_003749"
     CDS             complement(3020..3166)
                     /locus_tag="ACHKJF_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951028.1"
                     /translation="MAAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYR
                     GMSM"
     gene            complement(3634..4779)
                     /locus_tag="ACHKJF_003750"
     CDS             complement(3634..4779)
                     /locus_tag="ACHKJF_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFP0951029.1"
                     /translation="MKIVIAPDSFKESLSAMQVATAIEQGFREIYPEAEYVKLPMADG
                     GEGTVESMVEATEGHYLFQNVTGPLGTPVQARWGMLGDGKTAIVEMASASGLHHVPPE
                     CRNPMLTTSYGTGELILAALDHGVERIILGIGGSATNDGGAGMLQALGVAMLDTNGKS
                     LPFGGGALSNLALIDASGCDARLKNVSVTVACDVNNPLCGATGASAIFGPQKGATPEM
                     VASLEAALTNFGIRLQDATGRNVMTTPGAGAAGGMGAALLGILNAELKPGVEIVIDAL
                     HLDSLVADASLVITGEGRLDSQSISGKTPIGVARCAKRYQKPVIALAGGLTTDHQIVH
                     EHGLDAVFSVLTQIATLPEALQDAEYNLRVSARNIASVWKLSQQQMR"
     gene            complement(4787..6055)
                     /locus_tag="ACHKJF_003751"
     CDS             complement(4787..6055)
                     /locus_tag="ACHKJF_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004090684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0951030.1"
                     /translation="MVSITATGAIIALVVAIVLILRKVSPAYGMIVGALVGGLVGGAD
                     LVQTVTLMVTGAQGITNAVLRILAAGVLAGVLIESGAANTIAETVVKKVGESRALLAL
                     AIATMILTAVGVFIDVAVITVAPIALSIARRAGLSKLAILLAMIGGGKAGNVMSPNPN
                     AIAVSDTFHVPLTSVMMTGIIPGLFGLIVAYFLAKCLSQKGLMVMPDEVISHDNHGKQ
                     PGFLAAISAPLVAIVLLALRPVAGIVIDPLIALPVGGLVGAILMGRFKQSNAFMMSGL
                     TRMAPVAIMLLGTGTLAGIIANSELKDVLIHGLTSSGLPSYLLAPVSGAIMSMATAST
                     TAGTAVAAGVFSQTLLGLGVSALAGSAMIHAGATVLDHLPHGSFFHATGGSVGMLIHE
                     RLKLLPYETLVGFVVALISTLMFGVFGLAG"
     gene            complement(6175..7314)
                     /locus_tag="ACHKJF_003752"
     CDS             complement(6175..7314)
                     /locus_tag="ACHKJF_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid recognition domain-containing
                     protein"
                     /protein_id="HFP0951031.1"
                     /translation="MNNIHANYYLQDSTARQIVQRTMGIIPYSVNVMNEHGMIIASGE
                     ASRLHQRHEGAVLALTENRVVEIDSATANKLKGVKPGINLPINFRDQLIGVLGITGEP
                     GEVKAYAQLVKMASELIIEQMALLEQKQWDKRYREELINQLILRDPPPDSLNAVVSWL
                     GINLQLPRVVVIMELQQPDRDALHNLMEYFEYRARDHLVTFSDFNDLIILKPISLKNG
                     VWDKSLELSDLQRLKHYAQSCGFSRLIVGGYFSGENGLHRSFQTAKSTQSMAKRLKLK
                     HQFIFHDDYALASLLDGLSGTWQAEELSKIWRNLVAKDTKGMLQKTLQNYFDHNCDLS
                     QTATGLHIHVNTLRYRLQRVEDITGIQINNLKQLFWLYIGMELQR"
     gene            complement(7597..7955)
                     /locus_tag="ACHKJF_003753"
                     /pseudo
     CDS             complement(7597..7955)
                     /locus_tag="ACHKJF_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766597.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            complement(7956..8317)
                     /locus_tag="ACHKJF_003754"
                     /pseudo
     CDS             complement(7956..8317)
                     /locus_tag="ACHKJF_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
     gene            complement(8550..8789)
                     /locus_tag="ACHKJF_003755"
     CDS             complement(8550..8789)
                     /locus_tag="ACHKJF_003755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951032.1"
                     /translation="MSVVLLPPARPVLTRDMERVLERSGEAPVYWPNLHASFRLREDA
                     GFVPCVRLICSWPWHGYGQILLRNSIGVPDLLKVP"
     gene            8950..9219
                     /gene="yacA"
                     /locus_tag="ACHKJF_003756"
     CDS             8950..9219
                     /gene="yacA"
                     /locus_tag="ACHKJF_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015056448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin YacA"
                     /protein_id="HFP0951033.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
     gene            9219..9497
                     /gene="yacB"
                     /locus_tag="ACHKJF_003757"
     CDS             9219..9497
                     /gene="yacB"
                     /locus_tag="ACHKJF_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin YacB"
                     /protein_id="HFP0951034.1"
                     /translation="MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAG
                     QPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            9869..10399
                     /locus_tag="ACHKJF_003758"
     CDS             9869..10399
                     /locus_tag="ACHKJF_003758"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951035.1"
                     /translation="MVRSNTALTQVQALELIAQNSNFSSYHELIQVAKKTPLESRLLI
                     AAFGDSDFKDVIYNLYGSCTDGPYPSLEIAVEEELSDLIATTNADGFTIEDLEITDAF
                     YDDTKGILHLEVAFLYQGDQLPDHVYSGTEFEVEAKVRLSWRSEKWNFINEDFEVISV
                     VSDTERDWFEEESEEI"
     gene            complement(11584..11729)
                     /locus_tag="ACHKJF_003759"
                     /pseudo
     CDS             complement(11584..11729)
                     /locus_tag="ACHKJF_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612074.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(11790..12428)
                     /locus_tag="ACHKJF_003760"
     CDS             complement(11790..12428)
                     /locus_tag="ACHKJF_003760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951036.1"
                     /translation="MITYQNKNWKVNFIQRVGSGVPGFLLTGTEYILIPPAFVAESLF
                     SPLGLEYFDEIKSSRFFIQYIHKYIVECKHNDKNVIDKYNSEKEFLFTQILKLTSKGK
                     LKGLADLDIYTNCNIQSQFSNQSHSRYAPASAALTIDEKLAKALRNSNTHHISSGKII
                     CGPGNEDDNKAKIEAFLEEYKRMRESEQRHRIAIEARRDFMKELISIGFFSE"
     gene            complement(12425..12889)
                     /locus_tag="ACHKJF_003761"
     CDS             complement(12425..12889)
                     /locus_tag="ACHKJF_003761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951037.1"
                     /translation="MKIEEISIRFKALKPKSKITFTKNLISLLDQIESALILEGPYRL
                     VLDSNIIMRLESYRQGNISEGLLSILLTFKLIKKLPFHFDLVVRPTVFYEYLRQKNLK
                     STHEHWIKFKELKNLVEKELGSKLFFDGIETYQGAEYYLQSIQNDAEKIKKP"
     gene            13037..13225
                     /locus_tag="ACHKJF_003762"
                     /pseudo
     CDS             13037..13225
                     /locus_tag="ACHKJF_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13439..13789)
                     /locus_tag="ACHKJF_003763"
     CDS             complement(13439..13789)
                     /locus_tag="ACHKJF_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0951038.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSTISQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(13792..14754)
                     /gene="parM"
                     /locus_tag="ACHKJF_003764"
                     /gene_synonym="stbA"
     CDS             complement(13792..14754)
                     /gene="parM"
                     /locus_tag="ACHKJF_003764"
                     /gene_synonym="stbA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0951039.1"
                     /translation="MLVFIDDGSTNIKLQWQENDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSDLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQKL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKTHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            14890..15195
                     /locus_tag="ACHKJF_003765"
     CDS             14890..15195
                     /locus_tag="ACHKJF_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951040.1"
                     /translation="MFVGLFLGFVWRVTKDVAVLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLAPVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRTR"
     gene            15272..15955
                     /locus_tag="ACHKJF_003766"
     CDS             15272..15955
                     /locus_tag="ACHKJF_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP0951041.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRFGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMISFYGWHRIDRFMAAWKRAGFSVAGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            15956..16177
                     /locus_tag="ACHKJF_003767"
     CDS             15956..16177
                     /locus_tag="ACHKJF_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951042.1"
                     /translation="MNYAGHEKLRAEVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEAYTEILELDSCFKD"
     gene            16071..16623
                     /locus_tag="ACHKJF_003768"
                     /pseudo
     CDS             16071..16623
                     /locus_tag="ACHKJF_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            16668..>17165
                     /locus_tag="ACHKJF_003769"
     CDS             16668..>17165
                     /locus_tag="ACHKJF_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951043.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVQ
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESD"
BASE COUNT         4532 a         3894 c         4066 g         4673 t
ORIGIN      
        1 tgaaagacaa agcagccgga gtatcagcag aaacggcaca gcaacgtata aaagaattcc
       61 actcagaaaa attttttcac tcactccgga gcacaacaac ctttgctggc agaaaataca
      121 caaacagcga tatgccgtcg ccgaaaaaaa tgaaactaat ggcagacacc atttctgcgg
      181 tttatctcga tggatacgag ggcagacagt aaggggattt accataatcc cttaattgta
      241 cgtaccgctg aaacgctctc atacgcgttc tgacgcaaaa acgcaaaaag acaaggaaac
      301 cccaggctaa accgaaaaaa tgcgtcagaa tcgttttcag gtacttgcat aactattcac
      361 aaaagcgatg cgcgatcgat ttcgactatt ccgacggacg aacaggttaa aatgataagt
      421 ttcctccctc cggatggaag gttcagcacg gggattttta cccccttcca tcaacctgaa
      481 tatgcatctg ggagtaccca ttagtttcta ctcaaacact atcgtagtgc tatctcaaaa
      541 tataaaacat accgatggcg taagcatttt aaatatgata aataagaata ttttatcagg
      601 gattaattat gacaaaacta tatatttacc ttgttcttgc tgcatcatta tcggcattat
      661 caactgacat gattgtgcct gcgttaccag aactacagaa agcatttcat gctgactact
      721 cattaattca actaagtatt agtggatttc ttattgtatt tgcatttagt caactgatat
      781 caggaaccat aggtgattat ttcggaaaag tacgcgtaat gactatatgc ttaattatat
      841 ttcttattgg cagtattgta tgttttatgg ccaatgattt aaatatgctt cttttgggta
      901 gaattctaca ggcaataggt gcaggtgccg gacctgtaat atcaaaagcc attgctaaag
      961 agtcttttcc accactaaag ctaaaaagag cactttctga catatcatca acaagtgcga
     1021 taataccatt aatagcacct ctggccggag cattaatact tgattattat aactggaatg
     1081 taatatttat tgtaatgggt gcatttagca ttctaaccat actcctgtca cctaaagaga
     1141 ctaataaaca ggagaaaaaa acacatcaag ataatgtaaa atttgtgact aaagatttca
     1201 tctgcgggac aatactcgtt tcactaatgc taagctcact attttgctac atatcaatat
     1261 cacctgcact atttatgagt gattatggat taaatacatt tcactactct gttgtttttt
     1321 ccctgtcagt tttgtttttc atcattggaa accaattgtc aaaaatcgaa aaaatatcaa
     1381 acccatgggt cttatttccc ctaaatgcaa tatcagttac accttttctt atattaaatg
     1441 atgcttttat aatatgtgtt attggagcaa tgattttcaa tttaactctt ggcgcatact
     1501 atccaatagc taatttcatc tcattgcaga taaacggttc acgaactggt ttagcagcat
     1561 caattacagg ctttactcaa acagtaagcg cagggacaat cagtttcctt tcagtaaaaa
     1621 taagcgataa tggagttttt ttgggaacca ctttaagtat tagttgtctt attcttgcat
     1681 tattgtcaat gttcgtaact attattatag ggaataataa aaaatgaatt ggcaaaagaa
     1741 aaagatagaa cgttcaatca aataccaaac aagagtattt aacgaacata taaatgaact
     1801 agtgatcacc agaagaaatg gtaaggtaat cactgttaat gatgggaggg agatgattga
     1861 gagtttgtgt catgctctta tcttggactg gaaacacatg aaaatctaaa acatgcaatc
     1921 gctaatgcaa ttgaaagatt tggagtccaa ctttcggtcg ccagaacgcg cgtaaaagta
     1981 gatttattcg agcaattgga aaagagatta aataaaataa tgaatggggc gcacacatta
     2041 acatttaatg cggttacccc atgccattta gctgtactcc cacttcttgc atcaggaaca
     2101 ctacctaatt acaagtttaa taaaaaacca tattttattt tagagaaaac agcgcatgca
     2161 acattgcaaa taaatagagg actattacaa cagttcggtg aaacaatcag ggttgacttt
     2221 caacataaaa ataatataga agaagcgttt aaatatgctt caataaataa tatggtacca
     2281 atatctatat ctgatagtgt cggttcgatg ggtggggtgg ctcctgtcga atttattgtt
     2341 ggaatggcta acagatttga tgggtatgct tacattgatg atgctcatgg catgtcaata
     2401 tatgggaaaa atggctccgg atatgtaatg tcttgtctgg ataacaaact tgatgacaga
     2461 attattatcg ctggttcatt atctaaagcg tttggctctc atggtggatt tattgcatgc
     2521 aattatcctg acaccataaa ctttataaaa acgtatggaa ctacctatgc ttttggtggt
     2581 ccaccatcat taccaggaat tgctgcatgt atcgcagcag cagatttaca cctcgatggc
     2641 acagtatcct tgcgtcaaaa tgacttacaa caagttataa aatactttga tgacaaattt
     2701 aatgaaaccg aagtcataaa caaagggact acaatgccaa tacgtggatt gttaattggc
     2761 aatgaagaaa aagctataac gatatgcaaa aaaatgcatg atcgtggttt tgctacaacc
     2821 gttgctatgt atcctacagt ggaagaggga aaggcaattc tacgttgtgc aatatcagct
     2881 attcataccc gagaaaatat agatagccta cataaaaact ttatagaatg tattaacgaa
     2941 cagggttagt aaatatcatg gcctctttat agagaggcca tctatttcat ttttttatgg
     3001 cgtatagctg tccctgacat tacatactca tgccccggta gtgtccgggc cggcgttgtt
     3061 gttgcctgtg ctgttcgtcc cgcttccact catgccaggt ctgattaaaa ccgagttgac
     3121 ggaagagata atccggcatc tcttccggtt tgttgatggc agccaaaagc gagtcctgac
     3181 agcccttaag ccggaaccgc atcaggtgcg gttccggtgg gtacggagta tcagcatcac
     3241 ggtattgatg atatgaacgg cccaccggcc tgttgaggaa cctgtgaaac ggaaaggctc
     3301 attgaagccg tatattttct tgaggttcat caggcgcgga actcatcgag gcgcgggaat
     3361 aaaatcccat tcagacgccg gatagattca agcaagccca cttatcgtca aaatcggtgt
     3421 tgcaaaaacg ggagtgacca tagattcgtt tcccaaacag cccccttatt gaccattcaa
     3481 tttaaccgct taaccgtctt ttgggatcca ctactcccct ctttttttgc catgctagtg
     3541 ccttgcgtgg gatgactgaa gcaggctgtt ttagcatggg cctggtgaga aagaatatcc
     3601 aggccagcta ctgcttgccg gcctgactga agattagcgc atctgctgct gtgataattt
     3661 ccagacgctg gcaatattgc gtgccgaaac gcggaggttg tattccgcat cctgtagcgc
     3721 ttccggcaga gtggcgattt gcgtcagcac ggagaacacc gcgtccagcc cgtgctcatg
     3781 cactatctgg tggtcggtcg ttaagccgcc cgccagtgcg atcaccggtt tctgataccg
     3841 cttagcgcag cgtgcgacac caatcggtgt tttaccactg atgctctggc tatcaagacg
     3901 accttcaccg gtgattacca ggctggcatc cgcgaccagg ctatcgagat gcagcgcgtc
     3961 aataacaatc tccactcccg gctttaactc cgcattcaga atccctaaca gcgccgcgcc
     4021 catgccgcca gcggcacctg cgccaggcgt cgtcatcacg tttcgtccgg ttgcgtcctg
     4081 aagtcgtata ccgaagttgg tcagcgcagc ttctaaggag gcaaccattt ctggtgttgc
     4141 tcctttttgc gggccgaaga tggctgacgc tcctgttgcg ccacagagtg gattgttaac
     4201 gtcgcaggcg acggtgacag aaacgttttt cagtcgagcg tcgcagcctg aggcatcgat
     4261 gagcgccagg ttgcttagcg cgccgccgcc gaaaggaagc gatttcccgt ttgtatcaag
     4321 catcgccaca ccaagtgcct gtagcatacc tgcgccgccg tcattggtgg cgctaccgcc
     4381 aatcccgaga ataattcgct caacgccgtg gtccagtgcg gcaaggatca gttcgcctgt
     4441 gccataactg gtggtaagca tagggttacg gcattctgga ggaacatgat gtagtccgga
     4501 tgcggaggcc atttcaacga tcgccgtttt cccatctccc agcatacccc agcgcgcctg
     4561 tactggcgtc cccagcggtc cggtgacatt ctggaacaga taatggcctt cggttgcttc
     4621 aaccattgat tcgacggtac cctcaccgcc gtcagccatc ggcaatttga catactcggc
     4681 ctcgggataa atttcacgaa atccctgctc aatagcggtg gccacctgca tggccgataa
     4741 actttcttta aatgaatctg gtgcaatgac tattttcatc gtgtctttac ccggcaagtc
     4801 cgaagacgcc aaacatcagt gtggaaatca gggcgaccac aaaaccgaca agggtttcat
     4861 aaggcagcag ttttaaccgt tcatggatca gcatgccgac actgccgccc gtggcgtgga
     4921 agaagctgcc gtgaggcagg tgatcaagta cggttgcgcc agcatgaatc atggcggaac
     4981 cggcaagggc tgatacgcca agccccagca gcgtctggct gaataccccg gcggcaacag
     5041 cggttcccgc cgtggtggag gctgttgcca tggacatgat cgcgccggaa acaggggcca
     5101 gaagataaga aggcagaccg gaagaggtca gtccatgaat gagcacatct ttcagttccg
     5161 agttggcgat aatcccggcg agcgtaccgg taccgagcag catgatggct accggcgcca
     5221 tgcgcgtaag cccggacatc ataaaggcgt tactttgctt aaacctgccc ataaggatcg
     5281 caccgactaa cccaccgacg ggcagggcaa tcagcggatc gatgacgata cccgcaacag
     5341 gtcgtagcgc cagcagcaca atggcgacga gcggcgcgct gatagcggca agaaatcccg
     5401 gctgcttgcc gtggttgtcg tgtgaaatca cttcgtcggg catcaccatc aggcctttct
     5461 ggctgagaca ttttgccagg aaataagcca cgatcaggcc gaacagaccg gggattatcc
     5521 cggtcatcat cacggaggtg aggggaacat gaaaggtgtc cgaaacggcg atagcattgg
     5581 ggttaggcga cataacatta cccgctttac cgccgccaat catcgccagc agaatggcga
     5641 gtttcgacaa accggcacga cgggcaatag acagggcaat cggcgcaaca gtgataaccg
     5701 ccacgtcgat aaatactccg acggcggtca gaatcatcgt cgcaatagcc aaggctaaaa
     5761 gcgcccggct ttcccccact tttttcacca cagtttcagc gatggtgttt gccgcgccgg
     5821 attctatcaa cacgccggcc agtacacctg ctgcgagaat gcgcagcacg gcattggtaa
     5881 tgccttgcgc gccagtgacc attagcgtca cggtttgcac cagatcggcg ccgccgacca
     5941 gaccgcctac cagcgcaccg acaatcatgc cgtaagccgg cgagactttg cgtaaaatta
     6001 aaacgatcgc caccacgagt gcgatgatgg cgccagttgc tgttattgaa accatcttct
     6061 gctttctcca tgaaaataac agggggcagt atggagaaag cagccgcgtt agacttgtgt
     6121 gtatcggcta aaaatacgac gtcagacggc gaaaaatttg gagggaaata cagttcagcg
     6181 ttgtaactcc atgccgatat acagccagaa taactgcttt aagttattga tttgtattcc
     6241 tgtaatatct tccactctct gcagtcgata gcgtaatgta ttgacatgaa tatgcaagcc
     6301 agtcgctgtt tgcgaaagat cacaattatg atcaaaataa ttctgcagcg ttttttgcag
     6361 catacctttg gtgtccttgg caaccagatt gcgccagatt ttggataatt cttccgcttg
     6421 ccaggtacca gaaagcccat ctaataatga tgccagcgca tagtcgtcat gaaaaatgaa
     6481 ctgatgcttc aacttcagtc ttttcgccat ggactgggtg gatttcgcgg tctgaaaaga
     6541 gcgatgcagg ccgttttctc cagagaaata gccgccgaca atcagtcggg aaaaaccaca
     6601 ggactgggcg taatgtttaa gtctctgcag atcgcttaat tccaacgatt tatcccagac
     6661 gccgtttttt agcgagatag gttttaagat tatcagatca ttaaagtcgg aaaacgtgac
     6721 caaatggtcg cgggcgcgat attcaaaata ctccatcaga ttatgcagtg cgtcgcgatc
     6781 aggttgttgc agttccataa tcaccaccac ccgcggaagc tggagattaa ttcccaacca
     6841 tgagaccacc gcattcagtg agtccggtgg cggatcgcgt aaaatcagct gattaatcag
     6901 ctcttcgcgg taacgcttat cccactgttt ttgctcgagg agagccattt gttcaatgat
     6961 taattccgaa gccatcttca ccaactgtgc atatgccttc acttcaccag gctcgccggt
     7021 aatgcccagt acaccaatca gctggtcgcg aaaattaatc ggtaaattga ttccgggctt
     7081 aacgccttta agcttattgg cggtcgcgga atcaatttcg acgacccggt tttcggtcaa
     7141 tgcgagtacc gcaccttcat ggcgttgatg taaacgtgag gcttccccgg aagcaatgat
     7201 catcccatgc tcgttcatca catttaccga gtacggaata atccccattg tgcgttgaac
     7261 tatctggcgt gcggtagaat cttgcaggta ataattagcg tgaatattgt tcattttatg
     7321 ctctgcatga aaggcgggtg caatatgtgc tcatatccga tctattagcg tatccagtgg
     7381 tgaaatgttc cgtgtaaaca gacaaagaaa gcgggaagta cggcctcttt tttgtcggtt
     7441 tatacaaata aaaatgccac aaaaagcttg tatcctcttt tttcctttaa actcaaataa
     7501 ttaattatat ttctgttcgc ctgttccgtg ttttttttcg atgtgatcgt cataggatat
     7561 tggtaaggga gagcatgaaa aaacctgggg aagtagttat gctgccatcg cggataactt
     7621 tcctccactc tgagcgtcta tcgtgcaacc cgggtaccaa ctggcgaatt tgtcctgcag
     7681 ttaattattc aggacagcgg ttctggtctg taacaaataa tgcgtacctt ccctatccca
     7741 ctgcatctgt tgcttttggg tcattgtctg gcgatcactt cgttgattgt cgactctaca
     7801 aacgctgttg aaaccggttc accataccgg cgcatttttc cgtagtttgg gagcatcata
     7861 tgattattac ggatgcaggt atacatttca tccagctgtt tttgcaggga tttcaggttt
     7921 gcatagttga gttcagggtt atcgcagtac ataagctgca gcaattcctg atgatatgca
     7981 gtaatagcgt ttaccacccc tgacgtcatc cgtgtgtcgg cgatcgctga gtgtgccagc
     8041 ccttcccatg tcagtccggc ctgctggcag cactggtcag agaaatcgtg ccatagcggt
     8101 tggtggcccc ataatatcct gctgccagtc ccatcgcaca atatactgtc agttcttcca
     8161 gccagtcagc cggatcgcta tgtgcggcag cagtctgttt cagaatgcgg gtgtcgaacc
     8221 gggcattaaa gataattact ggttggtctc cgatcgcatg gcgcagttgc cgcgccacat
     8281 ctgtctatga tggcgcgccg cggagagcca gctcatttct tgacggtggg gcgtaattgc
     8341 tccattttcg tacaggttct cttccgcttc ctcgtcgatt cgctcactac gttcggtcat
     8401 cgatttgcgg cgagcggggt ctcctctcat atatcgttga cgattacgat ttgatttatt
     8461 tcgatcgagc cgttggaggc tcgttaaatg acttaaattg ttctgtaacg cgctcaatag
     8521 cgtcagggca tgggaggtga gaaaatgcgt tacgggactt tcaggaggtc aggcacgccg
     8581 atactgttgc ggagcagaat ctgtccgtag ccgtgccacg gccagctaca gatcagacgc
     8641 acgcagggta cgaagccagc atcttcccgg agacggaagg aggcgtgtag gtttggccag
     8701 tataccggag cttccccact tcgctccagc acccgttcca tgtcccgcgt cagcactggc
     8761 ctcgcagggg ggagtagaac tactgacatt cggacgactg taccggctta cgccgggatt
     8821 cggcggtttt actcctctgc catatacagc atggagtgcc gccttccatg ttgctggcgc
     8881 ggcatatcat ggtgacgata tctgaataat tatatactgt gtatatacgt agtaatgacg
     8941 aggtgatgaa tggcacaggt taatatgagt ttaagaatcg acgctgaact gaaggatgct
     9001 tttatggccg ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg
     9061 cggcagaccg ttgaacggca gcataatacc tggttccgcg accaggttgc ggcaggacgt
     9121 cagcaactcg agcacggcga tgtgcttccc catgacatgg tcgaatcctc tgctgcagca
     9181 tggcgggatg aaatgagcag gaaggttgcc ggtaaatgat ggagatattc tggaccatgc
     9241 tggccagcca ggacaggaag cgcattcgtg agtacattgc ggagcagaac ctgatggctg
     9301 ccattgaact ggacgaacgg attggttatt cagccagcag cctggccggg cagccgtata
     9361 aaggccgcaa cggtcgtgtg gaaggtaccc gggaactggt tattcacccc cattttgttc
     9421 tggtttatga ggtcgacagc cagtggggaa aagtgtatat cctgcgcgta ttgcatactg
     9481 cacagaagtg gccatagagg ctgtatggaa tcagttgtga atcatgacga agaaacattg
     9541 gggcgagcac tgataggggg cagtctggag aacggaaatt attgtacggt aagccagttt
     9601 tctaaccagg ctgagtgttg tttctcaatt tgacttaatc agctgatctt gccactataa
     9661 cgctaggtgg tgatggtcac ctggcgcctg tctcgcaaag caatagccca tctttattag
     9721 gttttaccta catcatactt ggctcgccac atttgtgacg ctcagagtcc ggttagcagg
     9781 ctatcggttc taaaagcgtc tgaaatttgg agaacagcca tgtcaaaata ttcttgtatt
     9841 aacattatca aaaccaaagc aaaaaaaaat ggtgcgctct aatactgcac tcacgcaagt
     9901 ccaagcgtta gagcttattg ctcaaaattc gaatttttcg agttaccatg aacttattca
     9961 ggttgcaaaa aaaacaccgt tggaatctcg cttgttgata gctgcttttg gtgattctga
    10021 tttcaaagat gtcatttata acttatacgg cagttgtacc gacggtccct acccttctct
    10081 tgaaatagct gtagaggaag agttatcaga cttgatcgca actaccaatg cagatggttt
    10141 taccattgaa gacctagaga ttacagatgc attttacgat gatacaaagg gtattttgca
    10201 tttggaagtt gcattccttt accagggcga ccagctacca gaccatgttt actccggtac
    10261 ggaattcgaa gttgaggcca aagttcggtt gtcatggcgc agtgaaaaat ggaattttat
    10321 caatgaggat tttgaagtca ttagcgtagt atccgatact gagcgtgatt ggttcgagga
    10381 agaatcagaa gagatctgac agtaggacgg taagcctgaa ttgatacagg cttacccata
    10441 gcaatagggg acactctgat gcgagatata aagagtgatc tcataggggt cagtttggat
    10501 atagataatt attgtacggt aagtctgttt tttgaacaga tttacctatc gcgatagggt
    10561 gtactcccgc gctatatggc atttttaggg aagaatttac ccgcgacact ctcaacgaac
    10621 ttttgtgcgc gtcagggcga gcaactctgg ttgctgattc cctgacgcgt gaacggattg
    10681 cagcctgtac gaactgtatt atcaataaaa ttaataataa cccgcagtcg tctgatgatt
    10741 gccatgacaa aatacttgaa gtacttagag caatgaacca gcatttgcgt cgttttgaat
    10801 attggtggtt gatccgatgg ggatttgcag tcatagcatg gtttaggtca ttgagagtaa
    10861 aaaaaagtat aaactgacaa aactgctttt tgactaatca cttggccgga aatttcggcg
    10921 gtgtcggcgg aaaaccccgt atatattgat gttggggttc caggccagaa aattccgtaa
    10981 ccaaagcagg taatcgacag atttacatga cgttgaataa gtgtctaatt ctgtatatct
    11041 acgcaagaaa tgcacggaag taagccaaac ggttatcgga tgctcagagc atttcgtaca
    11101 acctttacgt tcagtaagtt agctgatctg gatgtaacca ctcgttcaac tcaattctct
    11161 atacgccccg acttcgggta aaccctcgtc ggggccactc cgaccgtgca caaaaactct
    11221 gtcatggggt cagtttggag atcggaaatt tttgtacatt aagcgcatta tttaaccaac
    11281 ctagattcgt aacggattaa ttttgcatca gtggctatcg cttataagtt gtcgctcttg
    11341 cggtatcgca tttaggttat ccgattaagg tcaaacccct gaaaacctcg tatagcgcgg
    11401 gagtacaccc tatcgcgata ggcaagcctg ttcaaaaaat aggcttgccg tacaataatt
    11461 ctctatatcc caactgaccc ctgccaaacg ctaaagtttc aaatgaggag atggatgtgg
    11521 gtttcgatta tgaacgggca tacctttatc atcaatcaga tggtcatacg gttgctgcct
    11581 gctttagttg gctgaataca gcttgaaatc atcaccgtaa tgcaatgatg cccagcacag
    11641 ccttgcattt ttggcggcga tggccaaatc gaatcgactg gctgagtctg gctccgctct
    11701 ttcttgatac agaaagagcg gaggccctgg aaatcggcct taccgatgcg tccgaaggac
    11761 aggctagtaa attctgaaca aattttcgcc tactctgaaa agaaaccaat tgaaataagc
    11821 tctttcatga agtctctgcg tgcttcaatg gcaattcggt ggcgttgttc actttcacgc
    11881 atacgcttat attcttccaa aaatgcttct attttggctt tattgtcatc ttcatttcca
    11941 ggtccgcaga taatcttccc ggacgagata tgatgagtat tcgaatttct caatgctttt
    12001 gccagttttt catctatggt taaggcagcc gaagcggggg catagcgaga atggctttga
    12061 ttagaaaatt gactttgaat gttacagttc gtataaatat ctaaatcggc aaggcccttc
    12121 aactttcctt ttgaagttaa ttttagaatt tgagtaaata aaaactcttt ctcgctgttg
    12181 tatttatcga tgacattttt gtcgttatgt ttgcactcaa caatatactt atgtatgtac
    12241 tgaataaaaa aacggcttga ttttatttcg tcaaaatatt caagtcctaa aggggaaaag
    12301 agtgattcag ctacaaatgc aggtggaatc agtatgtatt ctgtgccagt taaaaggaat
    12361 ccaggaacgc cagaacctac acgctgaatg aagtttactt tccagttttt attttgatat
    12421 gtaatcaagg tttttttatt ttttcagcat cgttttgaat tgattgtaag taatattctg
    12481 ccccctgata tgtttcaatg ccatcaaaga ataattttga acctaactct ttctctacca
    12541 gattctttaa ttctttgaac ttaatccagt gttcatgagt gctttttaaa tttttttgac
    12601 ggaggtattc ataaaataca gtaggcctta ctactaaatc gaaatgaaaa ggtaattttt
    12661 ttatgagttt aaaagtaaga agaatcgaca ggagcccttc cgagatatta ccttgccggt
    12721 atgactctag acgcatgatt atattagaat ctaatactaa tctgtacgga ccttcaagaa
    12781 tcagtgcgct ttctatctga tctaataaag aaataaggtt cttagtaaat gttattttac
    12841 ttttgggttt tagtgcctta aatcgaattg aaatttcttc tattttcata tgaaaagtta
    12901 ttcagaataa atacttataa ccttgattaa tcttacacga ttatgtttgc agttgtcatc
    12961 gtgttagcgg ctctgacggc tcgttaacgc agagatacta aacccttgcc ggggtcatgg
    13021 cttacgccgg gcttgtatca catgtaactt accgctgaat gttagtgatt acgtgaccga
    13081 agcagttaat acagactcta attctattgc ggaaatgttt cataacgctg catggggagt
    13141 gctatcgttg tggtttgaac tcgtcataaa aatagatctg gatatacata aaaagaacag
    13201 gtatgcaagt tatgatttta gaagaaaaat cgaaatgcag catgaagagt ttcagaaaat
    13261 gacagagcgt gagcaagtgt ctttattgaa attgcctgaa taacagagac agtgcaaacg
    13321 attatttcat aataacgaca gtgtgtttat tttatgtaat gactggtaag aaaatagctt
    13381 ggttgagttc ccgttatcct gcatggtagt gggtaaaggg aactcaataa taattgaatt
    13441 agtttattag cttcatcgca ttttttttgg tctcttcatc acttttttgc tcttgttgtg
    13501 aagaggattg agaaattgtt gaagaattaa aatcggccat ctcttttgga aatagcgatc
    13561 tcaatatatt cacgatatct gaaaatgtgg tttctttcgt cagcagttca cataaaagga
    13621 aaggggtcct gggatcttgt ctgtataagg ccagacctgc cgtcagtgca gcccggttaa
    13681 ggcggcttcg ttccccttgc gggatactgt ccagtgtatc acaaacaatt ttatctgttt
    13741 gatttacatc tggatttagt ttgaaggcaa tggttctgcg cttgtccatc attaattacc
    13801 tatgagatac ataccgttaa ctaaatcata ttgagagtta ttggttttga aaaaacgttc
    13861 atcacgaatc tgtgtgtgtg tttttactgc atcgcatatt aattctgcgc caccacctat
    13921 aaccataaca tgagtataac cagaaaattc attgagcgta tttaatacac gttgctcaag
    13981 tttacgaagt gcttcattca ttgcttcggt gactattgat attttgttct catcattaat
    14041 tcgttgcttc agatagttgt tatcttttct gtgaataatt atatcgtcag caagatagct
    14101 acttcctttt gttctcgcaa gagaaagggc atcttttact gcagatgtga ccagagatac
    14161 accaagagat gagtctccgt atattttgct gatccctgat aatttaccca ttacctgaga
    14221 aatatctaat gtggtccccc cgagatctat aattaataaa gaatctaact catccagttt
    14281 ttgtagaact tcataacctg ccggtataga ttcaggcatg acttttacat cttttattgt
    14341 gaatgtatcc ccaccattta atgtaatttt tttccggaaa tttgcttttt tacgctcaat
    14401 attttccgta ttgggttggt tattcctgtc gtaatactct gtcagaggaa gtgtgcaaac
    14461 aatatccact tcgcttaccg gtagatcact ggtcagtaag gcgtggtgca ccgcaacgac
    14521 attaacgtcg ctgtattgcc atgcgatatt ggttgtgact acagcatccg ggctgattgg
    14581 atcaaatgaa tactgctcgc cgttcagtgt gtagttaaag acctttttat caccaaaaga
    14641 gactgcccac tcgcgtttga agctgttcgg gctaatgtgc tgtttaattg ttccgtcgtt
    14701 ttcctgccac tgtagtttga tgtttgttga accgtcatca atgaatacca acatttataa
    14761 aactccttat ggtgtttttg tggtgtgttt ttgggtgtgc tttgggtttt aaatggggtt
    14821 gtttgtcaag tttaccccat ttcaactatc aatcaatgat tacttgtatt gtttttggtg
    14881 tttattgggg tgtttgttgg gttgtttttg ggttttgttt ggcgagtaac aaaagatgtc
    14941 gctgtgcttc tctttttact gcgccggctg acgcggcgcg gcaggaacgc tgcctgtggt
    15001 cagtgtcccg cgtccggccg ggcacgggac gggcgctttt accgctcccg gctggcaccg
    15061 gtgacagtgt acgccagcct gtcgcccttt tctgatgaac gcccttcaag ccgctttcgc
    15121 ggcatagcct tgccgtcaga aagacgacgg ctgcggtatt ccacggtcgg cctgacccgt
    15181 taccggacgc ggtgaacagc ccacaggcag cggggaacgg gcaccacagg ggtgccctcc
    15241 cggtgtcctc tgtaagagaa ggagcgttgt tatgtcccgt tttgtcctcg gtaactgcat
    15301 cgatgttatg gcccgtattc ctgataacgc cattgatttc atactgactg acccgccgta
    15361 cctcgtcggt tttcgtgacc gtttcgggcg caccatcgct ggcgataaaa ccgatgaatg
    15421 gctgcaaccg gcctgtaatg aaatgtaccg cgtactcaaa aaagacgcgc tgatgatcag
    15481 cttctacggc tggcaccgta ttgaccgctt tatggcggcc tggaaacgcg ccggattcag
    15541 cgtggcaggg cacctcgtgt tcaccaaaaa ctacacatcc aaatctgcgt atgtcgcgta
    15601 ccgccacgaa tgcgcgtaca tcctggcaaa gggcagtccg gcccggcccc gtaacccgct
    15661 gccggatgtg ctgggctgga aatattcggg caaccgccat cacccgacgg aaaagcccgt
    15721 taccagcctg caaccgctga ttgagagctt cacacacccg aacgcaattg tgctggaccc
    15781 gtttgcaggc agcggctcaa cctgcgtcgc cgccctccag tccggacgcc ggtatatcgg
    15841 tatcgagctg cttgagcagt atcaccgtgc cgggcagcaa cgccttgccg ccgtgcaacg
    15901 ggccatgcag cagggggccg cgaatgatga ctggtttatg ccggaggctg cgtaaatgaa
    15961 ctatgccgga cacgaaaaac tgcgcgccga agtggcagag gtggccaata ccatgtgtga
    16021 ccttcgggcc aggctgaacg atatggaaca tcgctgtcgc tttgattccg atgtgcttgt
    16081 tgaacgcctg gcacgccaga cgctgtatcg cgccaatcgc ctgttcatgg aggcatatac
    16141 cgaaattctg gaactggatt cctgcttcaa agattgaggg gagagagtat gtacggaaca
    16201 tgcgaaacgc tctgccgggc gctggcagca aagtatccgg gagacacgcc gctgatgctg
    16261 gttgtctggt ccccggaaga gattcaggca ctggctgacg gtatggaaat ttctctgacc
    16321 gatcatgaaa tcaggactgt cctggcccgc ctggaggaca tcccggaaga ccagcggact
    16381 gaatccggta tttcttccgg tgcggcgatg gagattatca aatacgtgag cgaaaaccgc
    16441 caggtgaccg tccctgctga actgctggcg tccctgattc agaccgctga acaggcactg
    16501 tggaaacgtg agtgggccgc ccgtgataac ggcctcgccg ttccggaatg cgtcacccgc
    16561 cgacaggcgg tggttaatca ggcccgcgcc ctgctgaaaa acaacacacg cgaaaacgac
    16621 tgatgttatc gccgcctgcg ggcggcgatt cagggagacc atgaatcatg aacgaaacgc
    16681 tgaatgcact gatttgtcgc cacgcccgta acctgctgct ggcgcagggc tggccggaag
    16741 agacggatgt tgaccagcga aatccgaact atccgggctg gatcagcatt tacgtgcagc
    16801 tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cgatggcgtg ctgccgccgc
    16861 acctggcctc cgccattcag aaactgaccg gaaccggagc ggaacttgta ctgtccggca
    16921 gtcagtggca ggcgctgccg gtgcttccgg cagacggaac gcaggtgtct ttcccgtatg
    16981 ccggtgaatg gctgaccgaa gacgaaatca gggccgttct tgatgcggtg cgcaatgcag
    17041 tacgaagcat ctgttaccag gtggcagacg atgcgcggcg tatccgggcg gcgctgacca
    17101 caaccggtca gacgttgctg acccgccaga cccgccgctt tcgcctggtg gtgaaggaaa
    17161 gcgat
//
LOCUS       DAZGXZ010000107        17088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000107 DAZGXZ010000000
VERSION     DAZGXZ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.107"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            69..296
                     /locus_tag="ACHKJF_003770"
     CDS             69..296
                     /locus_tag="ACHKJF_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951044.1"
                     /translation="MACNVFAFSISGGVSERGYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(373..615)
                     /gene="yfdY"
                     /locus_tag="ACHKJF_003771"
     CDS             complement(373..615)
                     /gene="yfdY"
                     /locus_tag="ACHKJF_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP0951045.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            968..1888
                     /gene="lpxP"
                     /locus_tag="ACHKJF_003772"
     CDS             968..1888
                     /gene="lpxP"
                     /locus_tag="ACHKJF_003772"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP0951046.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2244..2315
                     /gene="ypdK"
                     /locus_tag="ACHKJF_003773"
     CDS             2244..2315
                     /gene="ypdK"
                     /locus_tag="ACHKJF_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP0951047.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2380..3618)
                     /gene="alaC"
                     /locus_tag="ACHKJF_003774"
     CDS             complement(2380..3618)
                     /gene="alaC"
                     /locus_tag="ACHKJF_003774"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP0951048.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3995..5692
                     /gene="ypdA"
                     /locus_tag="ACHKJF_003775"
     CDS             3995..5692
                     /gene="ypdA"
                     /locus_tag="ACHKJF_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP0951049.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5707..6441
                     /gene="ypdB"
                     /locus_tag="ACHKJF_003776"
     CDS             5707..6441
                     /gene="ypdB"
                     /locus_tag="ACHKJF_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP0951050.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6454..7311
                     /gene="ypdC"
                     /locus_tag="ACHKJF_003777"
     CDS             6454..7311
                     /gene="ypdC"
                     /locus_tag="ACHKJF_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0951051.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7314..9809)
                     /gene="ptsP"
                     /locus_tag="ACHKJF_003778"
     CDS             complement(7314..9809)
                     /gene="ptsP"
                     /locus_tag="ACHKJF_003778"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0951052.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQVRRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9834..10871)
                     /gene="ypdE"
                     /locus_tag="ACHKJF_003779"
     CDS             complement(9834..10871)
                     /gene="ypdE"
                     /locus_tag="ACHKJF_003779"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0951053.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATSAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10871..11956)
                     /gene="ypdF"
                     /locus_tag="ACHKJF_003780"
     CDS             complement(10871..11956)
                     /gene="ypdF"
                     /locus_tag="ACHKJF_003780"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0951054.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11971..13218)
                     /gene="fryC"
                     /locus_tag="ACHKJF_003781"
     CDS             complement(11971..13218)
                     /gene="fryC"
                     /locus_tag="ACHKJF_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP0951055.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13240..13566)
                     /gene="fryB"
                     /locus_tag="ACHKJF_003782"
     CDS             complement(13240..13566)
                     /gene="fryB"
                     /locus_tag="ACHKJF_003782"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP0951056.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(13785..14750)
                     /gene="glk"
                     /locus_tag="ACHKJF_003783"
     CDS             complement(13785..14750)
                     /gene="glk"
                     /locus_tag="ACHKJF_003783"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP0951057.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14954..16210
                     /gene="yfeO"
                     /locus_tag="ACHKJF_003784"
     CDS             14954..16210
                     /gene="yfeO"
                     /locus_tag="ACHKJF_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP0951058.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16325..16651
                     /gene="ypeC"
                     /locus_tag="ACHKJF_003785"
     CDS             16325..16651
                     /gene="ypeC"
                     /locus_tag="ACHKJF_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP0951059.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16792..>17088)
                     /locus_tag="ACHKJF_003786"
     CDS             complement(16792..>17088)
                     /locus_tag="ACHKJF_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HFP0951060.1"
                     /translation="QGFIRFHIPLWVRRTVTMLPSFIVILMGLDPTRILVMSQVLLSF
                     GIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWVIVVLVVALNIWLLVGTALGL"
BASE COUNT         4136 a         4574 c         4280 g         4098 t
ORIGIN      
        1 cattttcagg ataactttct atgaaagtaa acttaatact tttcagctta tttttattgg
       61 tctctattat ggcatgcaat gtttttgcat tttccatttc tggtggtgta agtgagaggg
      121 gctataaaga gactgaaaaa acatcagcga tgacaaccac acactctaca aaacttcagc
      181 catcacaggc gattttgttt aagatgagag aagatgcgcc accattaaac ctcacagaag
      241 aaatcacgcc tacttatccg acaaaggcga attatcttat tcatcctgtg cgatagcgtc
      301 tgtcacatgg caggtgaaca atcatgtctg atgctgatgt gaacacctgc ccaaatgagc
      361 catcctgacc cactatcgcc tgccataccc caccataaag cgaatcatca agccacaagc
      421 cggggcaaga aacataaata tccccgtcac tagttcatta ccgccagtta accacatggt
      481 gaagtaataa atgagtgcag cgaggatcac catgccgaag aaagaagaga ccgccgatac
      541 cacgttcagt acctgaccga tgtagccgct tacgcagaca atacacaggg cgagaaacat
      601 ccataaataa atcatagctc aacctcctgc cgcgctaaaa aacctgtaac ttcagtcact
      661 ttaatagtaa gacaatcctt aacctctggc aatttttgct aagcatcaat gaaaacagca
      721 tgttaaatgc aagactgttg tgtacggaaa aatatttact ttgcacgata aataatcagt
      781 agctgaaagc agtcagcgag tgataaagta cagataccgc attcatgctc ctcgtggtta
      841 tgtcctgact agtctttaca ctctttacag gaaccattgt cgtacatgat ggcccaacca
      901 attgagtgta ccgctgtcga catggaatct tctgcgatac aacaattcgt atctacagaa
      961 ggtaactatg tttccacaat gcaaattttc ccgcgagttt ctacatcctc gctactggct
     1021 cacatggttt gggcttggtg tactctggct ttgggtacag cttccttatc ctgttctctg
     1081 ctttctcggc acgcgtattg gcgcaatggc gcgaccattc ctgaaacgtc gtgaatctat
     1141 cgcccgtaaa aacctggaac tttgtttccc gcagcattct gcggaagaac gcgagaagat
     1201 gattgccgaa aactttcgtt cactcggcat ggcgctggta gaaaccggca tggcatggtt
     1261 ctggcccgac agtcgcgtac gtaaatggtt tgatgttgaa gggttggata accttaaacg
     1321 cgcacaaatg caaaatcgcg gcgtaatggt tgtcggcgtc cattttatgt cgctggaact
     1381 gggcggccgc gtgatgggac tgtgccaacc aatgatggct acctatcgtc cacataataa
     1441 tcagctgatg gaatgggtgc agacccgtgg gcgcatgcgc tctaacaaag cgatgatcgg
     1501 cagaaataat ctgcgcggca ttgtcggtgc actgaagaaa ggtgaagcgg tatggtttgc
     1561 tcccgatcag gattatggtc gtaaaggcag ctccttcgcg ccgttctttg cggtggaaaa
     1621 tgtcgccaca accaatggca cctatgttct ctcccgtctc tctggcgcag ccatgttgac
     1681 cgtaacgatg gtaagaaaag cggattacag cggatatcgt ttgttcatca ccccagagat
     1741 ggaaggctac ccgacagatg aaaatcaagc cgctgcctat atgaacaaga ttatcgagaa
     1801 agagatcatg cgcgcaccgg agcagtacct ctggatccac cgtcgcttta aaacgcgccc
     1861 ggtgggagaa tcgtcgttgt acatttaaca aactgctctt actctttccc tcctcaaatc
     1921 ggccataata acctcatggt ttttatggct tttttcttaa ggttttttcc ttcgctgaat
     1981 atctaattat taccgtcaga aattaaactg tcgccgatat gagacacttg taagttacta
     2041 aaagtaaata agaaaatgtc tcttgtctcg tttcagtttt aggcgtacat tagcgccgtc
     2101 tcgagcagtt tagctcagaa ttctggggtg atgcccacgg taaaatacag ggcttttaag
     2161 gtcgctttgc ccggtgtcaa ctcactgtat caggtgtaat gaagtcattc aggcgtaaca
     2221 gtaattacgc ggagagatgt aaagtgaaat atttctttat gggcatttct tttatggtca
     2281 tcgtttgggc cggtactttt gccctgatga tctaaaagca aactgtgcaa tgaaaaacag
     2341 gagccatcag gctcctgttt gcatttctga cgacggtgtt tattccgcgt tttcgtgaat
     2401 atgtttgctg ctggcgggta gtaaaccgtc ggcacggaac atcgctttaa tcccacgaat
     2461 cgcctgacga atacggtcgc ggttttcgat cagtgcaaag cgaacatgcg tatcaccata
     2521 gtcgccaaag ccaatccccg gcgagacaca aaccttcgct tcgttaagga gcttcttggc
     2581 aaattccagc gatcccatgg ccgcatatgg ttccgggatt ttcgcccaga catacatcga
     2641 agccttcggc atttcaacca tccagcccgc ttcatgcagc cctttaacca atacatcacg
     2701 gcggcgtttg tactgttcgg caatgtcgcg cacgcactgt tgatcgccct ccagcgccgc
     2761 aatcgccgcc acctgcaacg gcgtaaaggt gccgtaatcg tgatagcttt taatacgagc
     2821 cagggcgctg accagcgttt tgttgccaac cataaagccg atacgccagc ccgccatgtt
     2881 gtagcttttc gacagcgtaa agaactcgac tgccacatcg cgtgcacccg gtacctgcat
     2941 gattgacggc gctttccagc catcgtagac gatatcggca taggccaggt cgtggaccac
     3001 cagcacatcg tagcgtttcg ccagcgccac aaccttttcg aagaactcca gctccacgca
     3061 ttgcgcggtt ggattcgacg ggaagccgag gatcatcatc ttcggtttcg gataactttc
     3121 acgaatggcg cgctccagtt cgttgaagaa atcgacacct tccaccagcg gcactgagcg
     3181 tacctgcgcc ccggcaatca ccgcgccata aatatggatc gggtaacttg gattcggcac
     3241 cagcaccgtg tcaccatgat ccagcgtcgc cagcatcaga tgcgccaggc cctctttcga
     3301 accaatagtg acgatggctt ctgattccgg gtcgatttca acgtcgtagc gatcctgata
     3361 ccagcgggaa atggcgcgac gtaaccgcgg aatgccgcgt gaagtggagt aaccatgcgt
     3421 gtccgggcgc tgggccacag tacataattt ttcgacgata tgcggcggag tcgcaccgtc
     3481 cgggttaccc atgctgaaat cgataatatc ttcgccgcgc cgacgcgcag ccattttcag
     3541 ttcagcggtg atgttaaaaa cgtagggcgg gagacgatca atgcgcgtaa agcgacgttc
     3601 agggcgagtg tcagccataa tttcctcaga ttaacgttag cgcccggacc gtccgagcga
     3661 cgctgccaca atggtggctg ttttgaaaat agcctgatta atttctgtct gtccagcgtt
     3721 ttagaacatt tttatcaatg gctttaaatg aggaactcca cttcaaaaac aggaaatttt
     3781 tctacgctaa caagaaaatt accaccaatt ttattaacat tataatttca ataaatttac
     3841 cagccagaag tgcattatca agagatacat tgctaataca ccatttatct ttcaatcccc
     3901 ttttaaattt atggtttttc ctcaaaaacg gcctgctggt catcagcctt caggttacct
     3961 atcatagagg tttaatcctt attcagagtc acccgtgcac gaaatattca acatgctgct
     4021 ggcggtcttc gatcgggcag cgttaatgct tatctgcctg ttctttctca tccgtatccg
     4081 cctgtttcgc gaactgttgc acaagtcggc gcactcccca aaagaattgc tcgccgttac
     4141 cgccattttc tcgctgttcg ccctgttcag cacctggtcc ggcgttcccg tagaaggctc
     4201 gctggtgaac gtacgtatta tcgcggtgat gtccggcggg attctgtttg gcccgtgggt
     4261 aggcatcatt accggcgtga ttgcgggtat tcaccggtat ttaattgata tcggcggcgt
     4321 gacggcgatc ccctgcttta tcaccagcat tctggcgggt tgtatatcag gctggataaa
     4381 cctgaaaatc cccaaagcac agcgctggcg cgtcggtatt ctcggcggca tgttgtgtga
     4441 gacgttgacc atgattctgg taattgtctg ggcaccaact accgcgttgg ggatcgatat
     4501 cgtctctaaa atcggcattc caatgatcct cggtagcgtc tgtatcggct ttattgtgct
     4561 tctggtgcaa agcgttgagg gcgaaaaaga ggccagcgcc gcgcggcagg ccaagctggc
     4621 gctggatatc gccaacaaaa cgctacctct gtttcgccat gtcaatagcg agtcattacg
     4681 caaagtttgt gaaattattc gcgatgacat tcacgccgat gcggtggcga ttaccaatac
     4741 cgatcatgtg ctggcctatg ttggcgtggg tgaacataac tatcagaatg gcgatgactt
     4801 cattagcccg actacccgtc aggcgatgaa ttacggaaaa atcatcatta aaaacaatga
     4861 tgaagcccac cgcacaccag agattcattc catgctggtg atcccattgt gggagaaagg
     4921 cgttgtgacc ggaacgctga aaatttacta ctgccacgcg catcagatca cctcgtcatt
     4981 acaggaaatg gcggtcggtc tgtcgcaaat catctccacc caactggagg tttcacgcgc
     5041 cgagcagcta cgtgaaatgg caaataaggc agagcttcgc gccctgcaaa gcaaaattaa
     5101 tccccatttc ctgtttaacg ctttgaacgc tatttcatca tcaatccgtc tgaatccgga
     5161 tactgctcgc cagctgatct ttaatctgtc gcgttatctg cgctataaca ttgaattaaa
     5221 agacgatgag caaatcgata tcaaaaaaga gctgtatcaa attaaagact atattgccat
     5281 tgagcaggcc cgctttggcg acaagctgac ggttatctat gatattgatg aagaggtgaa
     5341 ttgctgcatt cccagcctgc tgatccagcc gttggtggag aatgccattg tccacggtat
     5401 tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt gcagagtgcg gcaatcgggt
     5461 acgcattgcg gtgcgggata ccgggcacgg catcgatcca aaggtgattg agcgggtcga
     5521 agcgaatgaa atgccgggca ataaaattgg cctgctgaat gttcatcatc gcgtgaagtt
     5581 attgtatggc gaggggctgc atatccgccg cctggaacca ggaacggaaa ttgcgtttta
     5641 cattcctaac caacgcaccc cagtcgcctc acaggctacg ttattgcttt gagccggagt
     5701 gatattgtga aagtcatcat tgttgaagac gaattcctgg cacaacagga actgagctgg
     5761 ctaattaaag agcacagcca gatggagatt gtcggcacct ttgacgacgg tctggacgtg
     5821 ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc tggatatcaa tattccgtcg
     5881 ctggatggcg tgttgctggc gcaaaacatc agccagttcg cccataaacc gtttattgtg
     5941 ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg aactggaggc gtttgactac
     6001 attctcaaac cgtatcagga gtcacgtatt accgggatgc tgcaaaaact ggaagcggcc
     6061 tggcaacaac agcagaccag cagtacgcct gccgcgacgg taacgcgtga gaatgacacc
     6121 attaatctgg tgaaagatga gcgaataatc gtcacgccaa ttaacgatat ctattacgcc
     6181 gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg aatcctacgt aatgccgatg
     6241 aacattaccg aattttgcag caaactgccg ccgtcgcatt ttttccgctg ccatcgctca
     6301 ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt ggtttaataa cacctacatt
     6361 ctgcgactga aagatctgga ttttgaagtg ccggtcagcc gcagcaaagt gaaagaattt
     6421 cgccagttaa tgcatcttta atcgggaact ttcatgaaag caccaggctt gcccgccgat
     6481 caacaatttt tcgccgatct gttcagcggc ctggtgctta acccgcaact actggggcgc
     6541 gtctggtttg ccagccagcc tgcctcgtta ccggtgggca gtttatgtat tgattttccc
     6601 cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc tggaagcaaa gcagcagcga
     6661 atggtggaag gagaaatgct gtttattccg gcacgcgcgg ctaatttacc gatcaacaac
     6721 aaaccggtga tgctgttaag cctggtgttc gcaccgacct ggcttgggtt atcgttttac
     6781 gatagccgca ccacgtcgtt gttgcatcct gctcgccaga tccagcttcc cagcctgcaa
     6841 cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc ttagccgttc gccgctggag
     6901 cagaatatca ttcagccact ggtgttaagt ttgctgcatc tttgccgtaa cgtggtgaat
     6961 atgccgccgg gcaattcgca gccgcgcggc gattttctct atcacagcat ttgtaactgg
     7021 gttcaggata attatgccca gccgctcacc cgcgagagcg tggcgcagtt ttttaatatc
     7081 acgcccaatc atctgtcaaa actgtttgct cagcatggaa cgatgcgttt tatcgagtat
     7141 gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc agaaatatca tctgtcaatt
     7201 catgaagtgg cacagcgttg cggttttccg gatagcgact atttttgtcg cgttttccgg
     7261 cgtcagtttg gtctgacgcc gggagagtac agcgcccgtt ttcagggcta acgtcagaag
     7321 gttaattctg tttccagcag cgtcaggata ctttgtgcat cttgcgcggc aaacaacgac
     7381 tggcggaagt ttttattcac cagtttacgc gccagctgcg agaagacttt cacatgattc
     7441 atcccttcgt tagcacccag cgtcagcatg atcaccagtt cgacttcgcc catttctgac
     7501 tgccagtcaa tcggtttcgc cagccgggca atgctgatac tggaatgacg gatccactga
     7561 gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg tggtaacaat ctcttcccgc
     7621 tgccagacgt cttcttccag ctcaaacgga tgttcagtgc gcccgttaac gccgaggttg
     7681 ccgcacagga actggatcgc ctgctcttta ttgctaaaat cctgatcaac aaagatattc
     7741 tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa aggcggtgag taacgcttca
     7801 atttcctgcg cactgcggca ttcacatgcc tgacgcgcca gttcccgaca cgcctcgcta
     7861 tccagttgac gaagctggct tttcaccgcc ggaatacgcg ggctactcat actcagctca
     7921 tccaggccca gcccaagcag tagcggcaga taacggcttt caccgcccag ttcaccgcaa
     7981 atgcctaccc atttgccccg ctgatgcgca gtggtaacaa tctgctgcaa catgcgcagg
     8041 aacgatggcg taatcgggtt atacagcggc gatacgcgcg ggttattacg atcgaccgca
     8101 tacagatact gggtcatatc gttggagccg atactgaaga aatcgacctc atcgcagaag
     8161 tgatcgatga tgtagcacac cgacggcact tccaccatga tccccagcgt aatcgtctct
     8221 gcatgacgca ggccatcgcg cttaagctca acgatcgctt tttggatctc gccctttacc
     8281 cataagatct gatcgagact gtggaccatc gggatcatca actgggcgtt gccaaaactg
     8341 gcggcgcgca aaatcgcccg tagttgagtg cggaacaggc cagcaaattc cgggtaaata
     8401 cgtaccgcgc gatagccgag gaacgggttc tcttcctggg gaatattcag ataaggaatg
     8461 cttttatcgc caccgatgtc cattgtgcgg aagataatcg gcttgtcgcc cgctgccagt
     8521 agcacctgct ggtaggcttc aaattgctcc tgctcgtccg gcgcgctgtc gcgatccata
     8581 tagagcattt cagtacggaa cagtccaacg ccttccgcgc cgttggcaaa cgcgcctggc
     8641 gcttccagag cggtgccgat attcgccgca atatcaatac gcttgttatc acgggaatag
     8701 gccagctgcg cggcggcctg cgcctgttgt ttttggcgtt tatccgccag cgtctgtgcg
     8761 acctgataat aaccgctcac cgcgtcattc gggttaatcg ccagcacgcc gcactgggcg
     8821 tcaagcaccg ccggttgccc ggcataacgg gcaatcgcat ccagcggcag gccgctcagt
     8881 accgggatcg ccgaggcgcg ggccagaatc agggtatgcg aggtgcgccc agttttctcc
     8941 agaatcatgc ctgcaagatt ttttaaatcg aggctcaaaa actggcttgg ggttaaatcc
     9001 tcggctacca gaatagtcgg tttttccagc accagattgt tgcgcggctt cagttccggc
     9061 caggtgatat gcagcaactg ttcgctaata tcgcgaatgt cgctaacacg ttcgcgcaga
     9121 taatcactgg cagaagcaga aagtttggcg caaacctgct ccatattgct gatgatcgcc
     9181 gcccccagcc cttgatgctg ttctgtcatc agacgacgga tattgcccgc gaattcatca
     9241 tcctgaatca gcgacaaatg ggcgctgagg atagttttgc tttcgccgtc acgctcacgc
     9301 aattgctggt tcagttgctc ggcaagcgtt gccaggctgt gctccagccg ggtggaatct
     9361 tgcgcactgg cggggattgc ccgataactg tcgaggctgt cgctttgtaa cagggtcagc
     9421 gtacccacgc cgacgccgct tgccagcaca ttgccgtaca gtaaatccgg gttaaggcgg
     9481 cttaatgaac gcggcagcgg atgcgccgtc agttctgcct gcgtcggctg aacgctgtcg
     9541 ctgtcgataa agcgcaccag gatgtactct tccagcacgc gccgcacctg ctcttcatcg
     9601 ctgccgctaa tgttcaggct gcaactgtca ttaaataagg tgccagttcc aatcaacgcc
     9661 agcgagcttt tagcatcggc ctttgcgttc tggcgatggt tgaaaaaggt gatttcgctt
     9721 tgccactggc tgcactgttc tttaagttcc cacgccggac gagcatgtag accgttaggc
     9781 aaaggacaga gaaattgaat cgttaacata accgctccgg gttaatcagg agatcatctg
     9841 aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt gaataagggc agataaaagt
     9901 tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg ccgcgcaatg tccgtggcgg
     9961 gtggcaggcc ccattaccac tgtgggtacg ccagtaccgg ttaagtgcac cgctccgccg
    10021 tccgtgccgc cgttgctgaa catatctgcc tgcaacggta cgccaatttc agccgccacg
    10081 gtttcgatcc aggcggtaag ttttggcggc gcaatcagtg acttgtcgct taacaccagc
    10141 atcgggccgt taccaatctg gcgatggtta gccgcgccat aatcaaagtt tttcgcccag
    10201 caggcggtat ccagcacaat ggcgacgtcc ggcgacaccg cgctggtagc ggtttgccca
    10261 ccgcgtaatc ccacctcttc gctggaactt gccaccagcc acacctccgc aggcagttcg
    10321 gcatcgtgca gctcacgtag taacgtcacc agcagatagc aaccgaggcg atcatcaaag
    10381 gctttcccca tcacccgctg gtgagggaga acctgaaaag ttgtatcaaa ggtgacgcga
    10441 tcgcctggac gaattcccgc ctgcatcact tcgtcatacg agcgcgcacc aatgtccacg
    10501 cgcatggcgc tgacgtcatt cccctgccgg tcgccgtcaa gcaggcctgg aattttgcac
    10561 tcttcacggg tggtgatgcg caccggctgc agctggcggg cagccatgcg cacgttgcca
    10621 accggcagca catcaattgc cccttcgcgg gagatgctac gcaccataaa tcccacttcg
    10681 tccatatggg cgcagatcat caccttcgga cctgtcgatt cattgaggcg gatcagcacc
    10741 gatcccagac catcaaatcg cacttctttt tgcaggcgat ccgcttcttc cagcaggatc
    10801 tgccgcactt cctgttccga ggaggcgatc gcatctgcct cgctcaacgc ttttaatagc
    10861 gataaatcca ttatgcctct cccgtgagca acactgtttt cggcatggcg tagagcactt
    10921 ctgcgccttg cggggtaacc agcacaacat cttcgatgcg cacgccccct tgccctggca
    10981 aataaatccc cggctccacg gtcagtaaca tgcctggctg tagcgtcgtg gtgtcccgcg
    11041 gtgaaaaacg cggatcttca tgaacttcaa tgccgatagc atgaccggtg ttatgaccga
    11101 aatagtggct aaaacctgcc tcggtaatca cttggcgcgc ggcatcgtca acctgctggc
    11161 agcgcacgcc ggggcgaatt gcagagattg ccgcgagctg tgcctgcagg acaatctgat
    11221 agacgtcaaa cagcgggtga gattcggcgc tcaccccttc gccattcacc agcaaggtgc
    11281 gcgtcatatc agagcagtag ccctgataca gcgcaccaaa atcgagagtg acaaactcgc
    11341 ccgctgcaac aatcttgtcg ctggctttgc cgtgcggcag cgccccgcgc cagccgctgg
    11401 cgacaatggt gtcaaaagag gctttttctg cgccctgctg gcgcataaac cactccagtt
    11461 cagcggctat ctcgcgctcg ctcatccccg cctgaataaa gcggcgaata tgctccgctc
    11521 cgcgatcggc aatcccacag gcgaggcgga ttatctccac ctcctctggc gttttgattt
    11581 gccgcagcac atccggcgtg gcgctaacca gtttcgcatt gagttcagac ttccagcggt
    11641 gcgcggtttc ccaactcacc tgctggcctt caaaaccgag cgtttgcaac tgctcatcgg
    11701 caatgatttg attgacgata gtggtaagcg tgttcgtcgc gtcaagcaaa tgcagctggt
    11761 agccttgcgc acgggcttcc acctcaacgt aatagcgcga atccaccaga atgtgcgcac
    11821 tttcacggct aataaccaca tagcctgatc cggtggagat ccccagatgc ggctgtttgt
    11881 tctgccgtga ggagagaagc actgcatcca gttgttgcgc cttaagccag tcgcgcagcg
    11941 aagcgagtaa tgtcatctgg attttccttc ttacaggcta tcgattaaca atttgccttt
    12001 acggaacatc atcagacgca ggaacaccac catcagcgca gttatgactg cccccagcgc
    12061 gatccccgcc atatagacgc ccaggttagt gaccagcggc catgcccaga tagcggactc
    12121 cgggaaccat tgcactgcgc ccagccagac agcggcggtt gagccgacaa tagcgccgac
    12181 catatacgac ggaatggcgg tgatggggct ttccagcgca aacggaatcg caccctcact
    12241 gatccccata aacgccagga acatggcggt cttaccctgc ggataaagtt gagcgttgaa
    12301 caggcgtttg ccggttaaac gacggtcgat aatggtcgcc agccccagac caatcggcgg
    12361 aataacgata gcgatagaac gcgcggtaac cggtaatacg tggtcggtgg taaagctgaa
    12421 ggcaacgaaa cctgccgctt tgttgattgg gccaccaagg tcgattgcgg tcgccgcggc
    12481 aatccccatt gcatacatca gcgcgccctt ctcacctgcg gcagtcagca cggtacggat
    12541 accgccgttg atccagccac caaatggggt gatgacatag tacatcgcca gcatgacaaa
    12601 aatggcggaa agaatcggta gcaaaaatgt ggttttgaac gctaacagga aatctggtaa
    12661 ctggattttc tggttcatcc atttcacaag gtaaccagca acgatagata taatcagcgc
    12721 accaatgaag gtggacggca ccggcgaaga ggtcgcccat tgcatagtgc tgggatcgaa
    12781 gttcagcagt tgcgtcggct gggtagacat caagccgccg ataaaaccag ccgggaacgc
    12841 cagtttgccg ccgatagagt tcgccacaaa agcggcaaac atcggaatag caaagccaaa
    12901 caacacgccg ccgaaggact gcgacagcca ggcaaatttc agtaaggaga ggtcgaaacc
    12961 ggagaatttc ccgctattaa gagcatccat gatgccgatc tcagcaggaa ttttaagcca
    13021 gctataagca atcagctggc tgaaagcgag gatcacaccg cccataatta aggtcggcac
    13081 catgcgtgaa atccctgaca tcacatgctg cgggagttca ccccagaagc tgcttttaga
    13141 ggcctgcgga ttcttaactg ccgctgccgc accggatgcg ccaggcacaa cggttgcact
    13201 gcgtttttta atggccataa aaattatccc tgtcgaatat tactgttgtt cagaggcaat
    13261 catctcttcg atttctttga tgatgcctac agcgttttta attgcgtcct gcaaagtgat
    13321 ttcataaacg tcgcgtgatt cgaaacgttc gttatcttcc ggggtaactg ccacggaatg
    13381 gatgatgatg gtcgcttcgg cgatatcctg cgccgtcagg cgattctgga tgccgtccgc
    13441 gccctgggtt tcaattttca cttcataacc ggcttctacc gccgcttctt ccagcgcctg
    13501 agcggccata aaagtgtgag ccaggcccat cgggcaggca cataaggcaa tcagtttctt
    13561 actcatcgtt aattcctcgt tcagtgatag ttgacgagtg aaattatgaa cgtgcaaaac
    13621 aaatcgccgt taccagacaa ataatgcatt tactggagaa ttaatcccgc tgtatggaag
    13681 tgtgacgcag gtcggccttg tgaggggaca caaagaaaaa ttgaattaac tgtatgattt
    13741 aaaagattat cgggagagtt acctcccgat ataacaggaa ggatttacag aatgtgacct
    13801 aaggtctggc gtaaatgtgc accggaaccg agaagacccg gattgtcatg gacgatgaga
    13861 tacaccggaa tatcatggac atattcttta aagcgccctt tatcttcaaa tgcggcacgg
    13921 aaaccagagg ctttgaagaa ctcaaggaag cgcggcacga taccgcccgc aataaacacg
    13981 ccgccaaatg tcccgagatt gagcgccaga ttgccgccaa aacggcccat aatgacgcaa
    14041 aacagcgaca acgcgcggcg gcaatcggtg cagctatcag ccagcgcacg ttcggtaata
    14101 tcttttggct tgagattttc tggcaggcgg ttgtcagctt tcacaattgc gcgatacaaa
    14161 ttcaccagcc cagggccaga aagcacgcgt tccgccgaaa catggccaat ttccgcacgc
    14221 agtatttcga ggataatggc ctcttcttca ctattcggcg caaaatcaac gtgaccgcct
    14281 tcgcccggca agcttaccca acgcttatcg acatggacca gatgcgcaac cccaagcccc
    14341 gttccggcac cgtaaaccgc aataggctta ccttctaccg gttctgcgcc accaaactga
    14401 atcagatgct cttttttcag catcgggatc gccatcgata cagcggtaaa atcgttaata
    14461 atttccagat ggctaaaacc gagattcttt ttcatttcgg caattgagaa cgcccaggta
    14521 tggttggtca ttgccaccca gtcaccggta attgggcaag cgatggcaat acagccgtct
    14581 ttcacctcga ccttatgttc ttcaagataa acgcgaatga ccgcttcgag gctggggtaa
    14641 tcaagccctg aataggtctt agcctgcgag atttcaccac tggcaatatc acacagagca
    14701 agacgtgcgt tggtgccgcc cacatcaccg actaatgcat actttgtcat tcttcaactg
    14761 ctccgctaaa gtcaaaataa ttctttctca cactgtaaat acctgggggc ataacaacaa
    14821 cgccggaaag gcaggctccc tgtaaatatc gatctgggtc acacatttac tttatcgttt
    14881 cagcaccaat tgcagcgatg cctttttgca agctgggcaa actaagtatc tgaccccgca
    14941 taaggaatag aacatgctcc atccgcgagc cagaaccatg ttgttattat cgctccccgc
    15001 cgtggcaatt gggattgcgt ccagtcttat tctgattgtg gtgatgaaaa tcgcctcggc
    15061 attacagaat ttgctctggc aacgactgcc gggaactctg gggatagccc aggattcacc
    15121 tctctggatc atcggtgtat taacgctaac gggtattgcg gtggggttgg ttatccgttt
    15181 cagccagggt catgccggac cagaccccgc ctgtgaaccg ctgatcggcg caccagttcc
    15241 gccctctgcg ctaccaggac ttatcgtagc attaattctc ggtcttgctg gcggcgtcag
    15301 cctggggccg gaacatccga tcatgaccgt caatatcgcc cttgcggttg ccattggcgc
    15361 tcgtctgtta ccgcgcgtca accgaatgga gtggactatt ttagcctctg ccggaaccat
    15421 cggcgcgctg tttggtacac ctgttgcggc ggcgttgata ttttcgcaaa ccttaaatgg
    15481 cagtagtgaa gttccgctat gggatcgtct ttttgcgcca ttaatggcgg cagcagctgg
    15541 tgcacttact accggattat ttttccatcc ccatttttca ctgcccattg cgcattacgg
    15601 acagatggaa atgaccgata ttctcagcgg tgcaattgtc gcggcgattg ccatcgcagc
    15661 agggatggtc gccgtatggt gcttaccacg gttgcacgcg atgatgaatc aaatgaaaaa
    15721 tccggtgctc gtgctgggta ttggcggatt tattctcggt attctggggg ttattggtgg
    15781 accagtttcg ctgtttaaag ggctggatga gatgcagcag atggtggcaa atcaggcttt
    15841 cagcaccagc gattactttt tgctggcggt aattaaactt gccgccctgg tcgttgctgc
    15901 cgccagtggc tttcgcggtg ggcgaatctt cccggcagtg tttgtcggcg tggcattagg
    15961 gttgatgctg catgagcacg tacccgccgt accagcggca ataaccgttt cttgcgctat
    16021 tctcggcatc gtgctggtgg taacacgcga tggctggtta agtcttttta tggcggcggt
    16081 cgttgtaccc aataccacat tgctaccgct gctctgtatc gtcatgcttc cggcatggct
    16141 gttattagca ggtaagccga tgatgatggt caatcgtccg aagcaacagc caccccacga
    16201 taacgtttag caaaaaatgc tctcctttat gatttaagag ttatggcgat gatacggggc
    16261 tttacctccc cgtaatattg cgttaacagg ccgctgacgg cttcgatcgt gaaggagaat
    16321 aacaatgttc aggtcactgt ttctggcggc cgccctgatg gcatttaccc cgcttgcagc
    16381 aaacgcaggt gaaatcaccc tactgccatc aatcaaatta caaattggcg atcgcgatca
    16441 ttacggtaat tactgggacg gtggtcactg gcgcgaccgt gactactggc atcgcaatta
    16501 tgagtggcgt aaaaaccgct ggtggcgtca tgataatggc tatcatcgtg gctgggataa
    16561 gcgtaaagcg tatgagcgtg gctatcgtga aggctggcgc gatcgtgacg atcatcgcgg
    16621 aaaaggccgc ggacatggac accgccatta aacacattcg ccaatggaac acaatgcctg
    16681 atgcgacgct agcgagtctt atcaggccta caaaccggct ctttaacgcg tagggcggat
    16741 aaggcgttta cgccgcatcc gccaatagtg ccagatgcga cgctcattca actacaatcc
    16801 cagcgccgtc cccaccaaca accagatatt cagcgccacg accagcacta caatcaccca
    16861 gcctgtctgt tttacgcgtt tgctgttcac cagatcgccc atcaacttgc tgtcactggt
    16921 gaaaatcagc agtggaacca gcgccagggc gataccaaaa cttaacagca cctgactcat
    16981 aaccagaatc cgtgtcggat ctaatcccat cagaatgaca ataaatgacg gcaacatggt
    17041 gactgtacga cgcacccaca gcgggatatg gaagcgaatg aatccctg
//
LOCUS       DAZGXZ010000108        17076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000108 DAZGXZ010000000
VERSION     DAZGXZ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.108"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            281..1306
                     /gene="purR"
                     /locus_tag="ACHKJF_003787"
     CDS             281..1306
                     /gene="purR"
                     /locus_tag="ACHKJF_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP0951061.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(1303..2235)
                     /gene="punR"
                     /locus_tag="ACHKJF_003788"
     CDS             complement(1303..2235)
                     /gene="punR"
                     /locus_tag="ACHKJF_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP0951062.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            2348..3559
                     /gene="punC"
                     /locus_tag="ACHKJF_003789"
     CDS             2348..3559
                     /gene="punC"
                     /locus_tag="ACHKJF_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP0951063.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            3850..4998
                     /gene="cfa"
                     /locus_tag="ACHKJF_003790"
     CDS             3850..4998
                     /gene="cfa"
                     /locus_tag="ACHKJF_003790"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP0951064.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(5038..5679)
                     /gene="ribE"
                     /locus_tag="ACHKJF_003791"
     CDS             complement(5038..5679)
                     /gene="ribE"
                     /locus_tag="ACHKJF_003791"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP0951065.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQLGTEA"
     gene            5894..7267
                     /gene="mdtK"
                     /locus_tag="ACHKJF_003792"
     CDS             5894..7267
                     /gene="mdtK"
                     /locus_tag="ACHKJF_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP0951066.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVKPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(7308..8564)
                     /locus_tag="ACHKJF_003793"
     CDS             complement(7308..8564)
                     /locus_tag="ACHKJF_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0951067.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            8872..8948
                     /locus_tag="ACHKJF_003794"
     tRNA            8872..8948
                     /locus_tag="ACHKJF_003794"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8906..8908,aa:Val,seq:gac)
     gene            8953..9029
                     /locus_tag="ACHKJF_003795"
     tRNA            8953..9029
                     /locus_tag="ACHKJF_003795"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8987..8989,aa:Val,seq:gac)
     gene            9137..9442
                     /locus_tag="ACHKJF_003796"
     CDS             9137..9442
                     /locus_tag="ACHKJF_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP0951068.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            9568..11172
                     /locus_tag="ACHKJF_003797"
     CDS             9568..11172
                     /locus_tag="ACHKJF_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP0951069.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(11184..11996)
                     /gene="ydhT"
                     /locus_tag="ACHKJF_003798"
     CDS             complement(11184..11996)
                     /gene="ydhT"
                     /locus_tag="ACHKJF_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP0951070.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVASTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLDEQHQFVEC"
     gene            complement(12000..12785)
                     /gene="phsC"
                     /locus_tag="ACHKJF_003799"
     CDS             complement(12000..12785)
                     /gene="phsC"
                     /locus_tag="ACHKJF_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP0951071.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYCIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(12782..13450)
                     /locus_tag="ACHKJF_003800"
     CDS             complement(12782..13450)
                     /locus_tag="ACHKJF_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0951072.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(13514..14152)
                     /locus_tag="ACHKJF_003801"
     CDS             complement(13514..14152)
                     /locus_tag="ACHKJF_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP0951073.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(14165..16267)
                     /locus_tag="ACHKJF_003802"
     CDS             complement(14165..16267)
                     /locus_tag="ACHKJF_003802"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP0951074.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(16288..16914)
                     /locus_tag="ACHKJF_003803"
     CDS             complement(16288..16914)
                     /locus_tag="ACHKJF_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP0951075.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         4133 a         4455 c         4019 g         4469 t
ORIGIN      
        1 attaccacac aaatagtgat attacgcatt tttacacact gtgatgaaaa aatctcccgt
       61 catttataat gataagtgtt tttaccactt ccccttttcg tcaagatcgg ccaaaattcc
      121 acgcttacac tatttgcgta ctggccattg accccttcct gacgctccgt gtcgtttttc
      181 cggcgtaccg caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg
      241 attttgcagg agctgaagtt agggtctgga gtgaaatgga atggcaacaa taaaagatgt
      301 agcgaaacga gcaaacgttt ccactacaac tgtgtcacac gtaatcaaca aaacacgttt
      361 cgtcgctgaa gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac actactcccc
      421 tagcgcggtg gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt tgctggcgac
      481 cagcagcgaa gcggcctatt ttgccgagat cattgaagcg gttgaaaaaa attgcttcca
      541 gaaaggttac accctgattc tgggtaatgc gtggaacaat cttgagaaac agcgggctta
      601 tctgtcgatg atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc
      661 agagccgttg ctggcgatgc tggaagagta tcgccatatc ccaatggtgg tgatggactg
      721 gggtgaagca aaagctgact tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta
      781 catggccggg cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtca tccccggccc
      841 gctggaacgt aacaccggcg caggccgcct tgccggtttt atgaaggcga tggaagaagc
      901 gatgatcaag gtgccggaaa gctggattgt tcagggtgac tttgaacccg aatctggtta
      961 tcgcgccatg cagcaaatac tgtcgcagcc gcatcgccct actgccgtct tctgtggtgg
     1021 cgatatcatg gcaatgggcg cactttgtgc tgctgatgaa atgggtctgc gcgtcccgca
     1081 ggatgtttcg ctgatcggtt atgataacgt gcgcaacgcc cgctatttta cgccggcgct
     1141 gaccacgatc caccagccaa aagattcgct gggtgaaaca gcgttcaaca tgctgttgga
     1201 tcgtatcgtc aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc gcttgattga
     1261 acgccgctcc gtggctgacg gcccgttccg cgactatcgt cgttaatcac ccgttgcggg
     1321 agtctcttcc ggctcccgca gccactcctt attcagcgtc tcactatcgc cgagatactc
     1381 aagcagccag gttaacgcag gcgacatatc attttgctgc catgtcagac aacatgccga
     1441 atccggaaag gggttttcca gttctaatgc cacccacttc ccctcattaa gccacggttt
     1501 ggcgaaatgt gttggcacca tccctatgca taatcctgcc gagatacagg ttgccgatga
     1561 ttcccagtca ggcacgacaa ctcttttttg gttatccagc aaccaggtaa tacgtttagg
     1621 tagcgttcgc gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct
     1681 gaacgggcca tccatcaacg ccagcgggtg gtggctggca acaacgcaac tccagcttag
     1741 catccccata tcccggaagg cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat
     1801 cgccagttcc acgcgcccat cggaaagcgc atcccagaca ccgttaaaca cttcctgaaa
     1861 gaccagaagt tcgacgtcgt caaaatggcg ataaaaatca acgatcatct gccgtgtacg
     1921 ttctggcctg acaatattat ccactgcgat agctaactga ccgcgccagc cgttcgctat
     1981 ctgctgacat tgctggcggg tgatctgcat ttttttgaca acagagcgcc cttctttgag
     2041 aaaccacgct ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa agagcggtac
     2101 cgccagccac tcttccagct gacgcacggt atagctgacc gcagaaggaa cgcgatgcag
     2161 ctcctgtgcc gcagcgctaa aactaccatt acgcgctacc gcatcaacaa cttcgagtga
     2221 atattctgac cacatagtct gcctgcaaaa tttttgaaac cagtcatcaa atattaccgt
     2281 ttcacaacac taatttcact ccctacactt tgcggcggtg tttaattgag agatttagag
     2341 aatatacatg caacctggga aaagattttt agtctggctg gcgggtttga gcgtactcgg
     2401 ttttctggca accgatatgt atctgcctgc tttcgccgcc atacaggccg acctgcaaac
     2461 gcctgcgtct gctgtcagtg ccagccttag tctgttcctt gccggttttg ccgcagccca
     2521 gcttctgtgg gggccactct ccgaccgtta tggtcgtaaa ccggtgttat taatcggcct
     2581 gacaattttt gcgttaggta gtctggggat gctgtgggta gaaaacgccg ctacgctgct
     2641 ggtattgcgt tttgtacagg ctgtgggggt ctgcgccgcg gcggttatct ggcaagcatt
     2701 agtgacagat tattatcctt cacagaaagt caaccgtatt tttgcgacca tcatgccgct
     2761 ggtgggtctg tctccggcac tggctcccct gttaggaagc tggctgctgg tccatttttc
     2821 ctggcaggcg attttcgcca ccctgtttgc cattaccgtg gtgctgattc tgcctatttt
     2881 ctggctcaaa cccacgacga aagcccgtaa caatagtcag gatggtctga cctttaccga
     2941 cctgctacgt tctaaaacct atcgcggcaa cgtgctgata tatgcagcct gttcagccag
     3001 tttttttgca tggctgaccg gttcaccgtt catccttagt gaaatgggct acagcccggc
     3061 agttattggt ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg gttatggctg
     3121 tcgcgccgca ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc tggtgctgtt
     3181 tgcagtcagc gtcattgcga cctgggctgc aggcttcatt agccatgtgt cgctggtcga
     3241 aatcctgatc ccattctgtg tgatggcgat tgccaatggc gcgatctacc cgattgttgt
     3301 cgcccaggcg ctgcgtccct tcccacacgc aactggtcgc gccgcagcgt tgcagaacac
     3361 tctacaactg ggtctgtgct tcctcgcaag tctggtagtt tcctggctga tcagtatcag
     3421 cacgccattg ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg tcgctctggg
     3481 ttacatgatg caacgttgtg aagaagttgg ctgccagaat catggcaatg ccgaagtcgc
     3541 tcatagcgaa tcacactgat ctatatcgat atacttatac ttaggctgct aacaaaattt
     3601 tgttgtatga ttgaaattag cggcctatac taatttcgag ttgttaaagc tacgataaat
     3661 attatgtttt tacggggaca ggatcgttcc cgactcacta tggatggtca tttcggcaaa
     3721 ggttcctcct ttccctctgt tctacgtcgg attatagact cgcggttttt tctgcgagat
     3781 ttctcacaaa gcccaaaaag cgtctacgct gttttaaggt tctgatcacc gaccagtgat
     3841 ggagaaacta tgagttcatc gtgtatagaa gaagtcagtg taccggatga caactggtac
     3901 cgtatcgcca acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg
     3961 gatattcgtg tgaaaaaccc cgattttttt aaacgcgttc tgcaagaagg ctctttgggg
     4021 ttaggcgaaa gttatatgga tggctggtgg gaatgtgacc gactggatat gttttttagc
     4081 aaagtcttac gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga cacgctgcgt
     4141 attgccagcg ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaag
     4201 gagcattacg atttgggtaa tgacttgttc agccgcatgc ttgatccctt catgcaatat
     4261 tcctgcgctt actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa
     4321 atgatttgtg aaaaattgca gttaaaacca gggatgcgcg tactggatat tggctgcggc
     4381 tggggcggac tggcgcacta catggcatct aactatgacg taagcgtggt cggcgtcacc
     4441 atttctgccg aacagcaaaa aatggctcag gaacgctgtg aaggcctgga tgtcaccatt
     4501 ttgctgcaag attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg
     4561 ttcgagcacg tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg
     4621 aaaccggaag gtatattcct gctccatact atcggttcga aaaaaaccga tctgaatgtt
     4681 gatccctgga ttaataaata tatttttccg aacggttgcc tgccctctgt acgccagatt
     4741 gctcaatcca gcgaacccca cttcgtgatg gaagactggc ataacttcgg tgctgattac
     4801 gatactacgt tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat
     4861 aactacagtg aacgctttaa acgaatgttt acctattatc tgaatgcctg tgcaggtgct
     4921 ttccgcgccc gtgatattca gctctggcag gtcgtgttct cacgcggtgt tgaaaacggc
     4981 cttcgagtcg ctcgctaaag gctattctat cgccccctct ccgggggcga tttcagatca
     5041 ggcttctgta cctagttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt
     5101 atctaccact gcctgagttt gtggatcaat ttcaatgttg acgcgtgcgc cgagtttttt
     5161 cttcccaaga gtcgtgcgtt ccagagtttc cggaattaaa tggacgcaaa aacgcgttgg
     5221 cgtgacttcg ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat
     5281 atatttcatc aactgactat cctggacttt aaaccagatc tggcgattat tttccgaggt
     5341 taatattttc gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat
     5401 ttcgtcactg aatttcgccg cacgctcaac gtttacccaa tcccccactt ttaaatcgcc
     5461 aagattggta atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat
     5521 ttccgtcacg gtcaggcagc aaccgttatg cgccacggaa gcacctgttt ccaggccgtc
     5581 cagcatgtgg tcgggtaact ccaccacatg cgtacgaaaa tttggtttct cgtcaatcga
     5641 caccagtttt gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag
     5701 ttaagacatt ctgttcagca caatagcagg tggcaaacgc ccttaccagt gaaggggtaa
     5761 gaatgactat tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc
     5821 ctgcttcttc tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat
     5881 aaaaggtgtt cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat
     5941 cccggtgatt ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc
     6001 gggcggctat agtgccaccg acatggcggc ggtcgctatc ggtacttcta tctggcttcc
     6061 ggcgatcctc tttggtcacg gactgctgct ggcattaacg ccggttatcg cgcaattaaa
     6121 tggttccggt cgacgtgagc gcattgcgca tcaggtgcga cagggcttct ggctggcagg
     6181 ttttgtttcc gttctcatta tgcttgtgct gtggaatgca ggttacatta tccgctccat
     6241 ggaaaacatc gatccggctc tggcggacaa agccgtgggt tatctgcgtg cgttgttgtg
     6301 gggcgcgccg ggctatctgt tcttccaggt tgcccgtaac caatgtgaag gtctggcaaa
     6361 aaccaagccg ggtatggtaa tgggctttat cggcctgctg gtgaacattc cggtgaacta
     6421 tatctttatt tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc
     6481 tactgcggcg gtgtattggg tcatgttcct tgccatggtt tcttacatta aacgcgcccg
     6541 ctccatgcgc gatattcgta acgaaaaagg caccgcgaaa cccgatcctg cggttatgaa
     6601 acgactgatt caactcggtt tgccgattgc gctggcacta ttctttgaag tgacactgtt
     6661 tgccgtcgtg gctctgttag tgtctccgct cggtattgtt gatgtcgcag gacaccagat
     6721 tgccctgaac tttagttcac tgatgtttgt gcttccaatg tcgctggcgg cagcggtaac
     6781 tatccgcgta ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa ccgctgcgcg
     6841 gaccgggctt atggtgggtg tctgtatggc aaccctgacg gccatattca cggtttcact
     6901 gcgggagcaa atcgccctgt tgtacaacga caatcccgag gttgtaacgc tggctgcgca
     6961 tttgatgttg ctggcggcgg tatatcagat ttctgactca atccaggtga ttggcagtgg
     7021 gattttgcgt ggttataaag atacgcgttc cattttctat atcaccttta cggcttactg
     7081 ggtgctgggc ttgccaagcg gctatattct ggcactgacc gatctggtcg ttaaacctat
     7141 ggggccagca ggcttctgga taggctttat tattggcctg acgtcggcag ccattatgat
     7201 gatgctgcgt atgcggttcc tgcaacgtct gccgtcagtt atcattctgc aacgagcatc
     7261 ccgctaataa agacaaggcg caaccttcac gggttgcgcc tgtgttttta cgcaggctgg
     7321 agcgttgcgc caatcccgtc ttcgtctggc tgtaatttca gagcgttgca cagagtttgc
     7381 cgactggcat ctgttatctc aacgtgtata tccccggtaa cttccccttt cacattgatg
     7441 aagtcaggtg agatttcttt cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg
     7501 cgtttcactt tcagttgcac atgcgttgcc gcaccagtga tcagccacag ttttccgccg
     7561 ttcagattga tgttaccaac actaaaagtg acactgtcac cttttaccgt actgccgtcg
     7621 agaatcaaca caccgccctg ctgtacagta atcgttgatt gttgcagatc tgcgccatag
     7681 ccaataatca ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc aaccggtgcg
     7741 cgatcttcct ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag gatcactgtt
     7801 ccaccttgta ttaccgcatt ttccagcgta ccggcatgca cttcagcccg accacccaca
     7861 ttcacggaca ggttttgcgt cttaccggaa ctgtagagct gaaggccatc cgtccccaga
     7921 cgataaatgg atccattctc aacaatagtg tcagagtctt caccaccgag attattcatg
     7981 gtgccaccag caagcaacgt atcactggct ttcccggcca gcataaaaac gccgccctga
     8041 ttaatctcac aattgctggc ttccccacct ggatacacca acaattcacc accttcatct
     8101 accacgaccg ctgaggttgt cccattaacc aacaggccgc cctcttgatc gagaatgatt
     8161 ttttccgcgc gatgtccttc cagtacacgc aggttaccgc cattttccag caacaaaccg
     8221 caggcataac cctgatcaac gctgaattca ccttcgggat ggcggccatt aaccgtagcg
     8281 agcgtagaga gactaattgc gcctccctcg gactgaacaa tatctgtggc aatggcaccg
     8341 tcgctgaccg atagcgtgcc attgttaccg tactgtgtgc atttggcgat cccaccggtt
     8401 gccactttca accagccagc ccccgtgacc acggtatttt cggctcttcc gccagcttca
     8461 acaatcaact cgccttcagt aagttgcgtc gcgccaatga cctcgccggt cttaacaatt
     8521 tgcacattcc cgtcgctcat caagtttttc gcatcagatc ccatgattta ttcctttgct
     8581 gcatctgtgt gcctttattg ctacctaagt gtaaaggcta cggaggattt atccacgaca
     8641 gatttgagat ggtggcaaac aactctgttt aaactctgat acacgaatta ttgggttgta
     8701 tcagatgtaa atgcgatcct gaataaaaat cgcccttgca aatcaacaaa atatagccaa
     8761 tttgcttaaa agctctgcaa acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc
     8821 caccttccca ctctgccgct aatattcgtc cccgttgtca cctacaacgt tgcgttcata
     8881 gctcagttgg ttagagcacc accttgacat ggtggaggtc gttggttcga gtccaattga
     8941 acgcaccatc ctgcgtccgt agctcagttg gttagagcac caccttgaca tggtgggggt
     9001 cggtggttcg agtccactcg gacgcaccag attttcttaa tctggtcttc tcctttttcc
     9061 ctctgtttct tctctgtatc caatacgtta aaagatttac actgtcttcg tatgcgttat
     9121 cagaaggaga atcgctatgg caactttgtt acaacttcat tttgctttta atggcccatt
     9181 tggcgacgca atggctgagc agcttaaacc gcttgctgag tcgattaatc aggaacctgg
     9241 ttttctgtgg aaggtatgga cagaaagtga aaagaaccac gaagccggtg ggatctatct
     9301 ttttactgat gaaaaaagcg cccttgccta tctggaaaaa catactgccc gacttaaaaa
     9361 tctcggcgtt gaggaagttg tcgccaaagt tttcgatgtc aatgagccac tgagtcaaat
     9421 caatcaggca aaactcgcct gacagaattt aatcaagggc ggttagcgcc cttttcatcc
     9481 ctgtctgaaa tttctcaaat tctaaaaatc tcaaccaaac ttatctgata acactaaatt
     9541 cgaaagaatg cgtacaggta agtaacaatg aaaaaaattg ctattgtggg tgccgggcct
     9601 acggggatct acaccttatt ctcgcttcta cagcaacaaa ctccactttc tatttctatc
     9661 ttcgagcagg ctgacgaggc cggtgtcggg atgccataca gtgatgagga aaactcaaaa
     9721 atgatgctgg caaatattgc cagtattgaa ataccgccga ttaattgtac gtatctcgaa
     9781 tggctacaaa agcaagaagc cagccatctc cagcgttatg gcgttaaaaa agaaaccttg
     9841 cacgatcgtc agtttttacc gcgaattctg ctgggcgaat atttccgcga tcaattttta
     9901 cgattagtag accaggcacg acagcaaaaa tttgcagtgg ctgtttatga atcatgccag
     9961 gttaccgatc tgcaaattac aaatgctggc gtcatgctcg ctacaaatca ggatttaccc
    10021 agcgagacgt ttgatttagc ggtgattgcc acgggtcacg tctggcctga tgaagaagaa
    10081 gcaaccagaa cgtattttcc cagtccgtgg tcaggcctga tggaagcaaa ggtcgatgcg
    10141 tgtaacgtgg gtattatggg aacatccttg agcggactgg atgcggcaat ggcagtggct
    10201 attcagcatg gttcgttcat tgaagatgat aaacaacacg tcgtttttaa ccgcgataac
    10261 gcaagtgaaa agctaaatat tacgttgatg tcgcgcacgg gtattttacc cgaagccgat
    10321 ttctattgcc ctattcccta cgagccctta cacatcgtca ccgatcaggc attaaatgct
    10381 gagattcaaa aaggtgaaga gggccttttg gatcgggtat ttagattgat agtagaggaa
    10441 atcaagtttg ctgatccaga ctggagccaa cgcatagcct tagagagcct gaatgtcgat
    10501 tcctttgctc aagcctggtt tgccgagcgc aaacaacgcg acccatttga ctgggcagaa
    10561 aaaaatctcc aggaagtcga acgcaataaa cgagaaaaac atactgttcc ctggcgttat
    10621 gtcattctgc gcctgcatga agccgtacag gaaattgttc ctcatctgaa tgaacacgac
    10681 cataaacggt tcagtaaagg ccttgccagg gttttcatcg ataattatgc ggcaatccct
    10741 tcagagtcta ttcgtcgcct acttgcctta cgtgaagcgg gaatcattca tattctcgcc
    10801 ctcggtgaag actacaaaat ggaaattaat gagtcgcgca ccgtcctgaa aacggaagac
    10861 aacagctact cgtttgacgt ttttattgat gcccgcggac agcgtccgct taaagtgaaa
    10921 gatattcctt tccctgggct acgcgagcaa ttacagaaaa caggggatga aatccctgat
    10981 gttggcgaag attatacgtt acagcaaccc gaagatattc gtggacgcgt agcgttcggc
    11041 gcgttgccct ggttgatgca cgaccagcct ttcgttcagg gacttacggc atgtgcagaa
    11101 attggtgagg cgatggctcg ggcggtcgta aagcctgcat cccgtgctcg tcggcgtctt
    11161 tcgtttgatt aaaggtaaag ctatcagcac tcaacaaact gatgctgttc atcaagacga
    11221 tagcggactc cggttcgaat attgttttca ccttcaatgg cgacggcgaa gcgaactctt
    11281 tcgccgtcat gatatgccag tgcggcgctt ccccctggcc cgagaataat tgagtcgacc
    11341 acgcccgtgc tcgctaccgt tgaattttca ccgctggcaa tgatgtgtac attgtcgccg
    11401 ctgttggcaa ttctggcgtt tgcgccaaag ctggcgattt gcaccaggtc accattactg
    11461 gcgacatgac accgttcacc caatgtacaa acgcgtactc gcattccggt attcgcaatc
    11521 cgactactat ctccagcgct gcttatccgc gaagaattac cggttacagc aacccgtgcg
    11581 cgttcaccgg agctgccaat gtgactgtta aagccgacac ttccaatccg cacgctataa
    11641 cccatactgg caatttgcgc ggcataccca gcggttgcga ttcgggcgtt atcctcacaa
    11701 cagccgatgc gagcatgatc gtcgctgcgg gcaaactgac tgcaaaatgg tatctctccg
    11761 gggtctgtgg ccagtttttg catagaggaa acttcctgat gcgcgaagtt ttcatccgcc
    11821 agccattttt tccacgcata ctcaaccaga ctctccgccc agtccgtata tccttcctca
    11881 atcagtgcat gatgaatatc ggcataacta ccgccgcgtg ggaaatggcg caggaaccag
    11941 cgatacatca ccgctccgat acgccattct cgcaggtctg ctcgtgtaat gatcattcgt
    12001 tagtgccggt gatagccatc gaccatgctt ttaaaggttt cgtgtggcgt acgccccgtg
    12061 gtgcaaagat aaagatgacc gaagataaaa aagaggctta taaatgccag agcaaaatgc
    12121 gcctgcaata accagtatct tacgccagga aacacatctc ccacggcttg cggatagaga
    12181 cacagcagcc ccgtcaatag taacaacggc agcaatccat acataacacc aacataggcg
    12241 acctgctgta agggattaaa tttagactgg gttgttgccg ggaaaggatg ttcttccccc
    12301 tgcataatgc caaacaaata aaatcgcgtt tgttttgccg ctcgctccag ccacccctga
    12361 cgacgaatgc aatagtggtg accattaccc ccaacggcat tgatcagcac aaagccgagc
    12421 cagcatgcga gtaacaaaaa tccgcaaact tcatgcaccg caaccagact tttaaccgca
    12481 gttgcgccca ccagcgcaaa gtgatttatc agcccacttg ccagcaacaa tacaaagagt
    12541 aacgcattcg accagtgcca taatctgacc gctttgcagt aaagataaat cttctcacca
    12601 taatcggtga ctgatttctt cacgccacga gcacgaagca aggcatgcag ccccagcacc
    12661 aaccacatcc caaccagcaa tactccggca atgatcaacc acaccggcca aaattcggga
    12721 gtgaactgtg gcacatagtt cgctaactgg ctctgaaact gttcagcatg ttgcgacggg
    12781 ttcatacatt ttccttttta atcaaatgtt gaccgaaccg gcgatacaga tgcggtttcc
    12841 cggcaccagg tagctgatat tgatagtatt tattttgctg caaccacgcc tgaatttctg
    12901 ggctatcttc gcgaccaaag atcaatgcgt gttccgggca ggcgctgacg caaatgggcg
    12961 gaaagccttt tgccagtcgg gactcagcgc agaaatcaca tttatctgcc actttagtga
    13021 cagggttaag gtagcgaacc tggtacggac aagcgccaat gcaataactg cagccgatgc
    13081 actgcgattt ttctacccgc acgatcccct gctcatcacg ccacgacgcc cctgtcgggc
    13141 atacgtcgat gcacggagca tcttcgcagt gctgacatga ctggcgaaaa aagtgatact
    13201 gcgtttcgtt gtcgttatcg gtgactggaa tgtgtgctat cgataaacgg cttccctgag
    13261 cggggacgtg gttagtttta cggcatgcgc gggcgcaaat attacagcca ttgcaccgtg
    13321 actcgtcatg aatcatggca taccgaactt ccttttcttg cctcgtgttc gctaacagag
    13381 aagacgcaga accagtaaaa aagattactg ttcccatccc cagaacaaat ttgcgtcgag
    13441 tgaaggacat ctgattatcc cctaatgcag tggctgcaac tgtccaccct atccatgaca
    13501 tctccctgaa atttcagggg ttctgaaact gctctacctc gaaccattcg cataagccat
    13561 aagccttgcc ataggtcata aatcgctcgc taaacaagta caacaccgcc gtcgatgact
    13621 ccacctgccg tatatcggca tattcaggtg caacatccat cgcggcgatc gcggcttcaa
    13681 tctgcgcttc ctggaagtaa tatggcgctt gcatcagcat ggcgactttg taaggacgag
    13741 gatatgtctg gcactcaaaa cgaacggcgt gtgcaatggc gcgacaaatg tcctgctcca
    13801 caacttgcaa agacatcgcc gcgtagtttt cactcatggc ctgcgttgag taatagtaat
    13861 catcctggct tcccgtcata caagcgatat ccgcgtagtc tccgttctgc ttaatgcttt
    13921 ccagcaaaac tgacagctcc tcttctgcaa ccgaataggg tggcgagaga aacacggcgc
    13981 gcgccaccag ctgcccagcc gcagaatgct ggcgaatgaa ctctgccagg atttcagccg
    14041 gttctggttc ggttaccgta tcaacggggt gacgcatccc ctgtaaccat tgccgcttcg
    14101 cctcgtcaac ttcgctgact ttcgctaacg gcaattcatc ccgatggttc atctcaccca
    14161 ttccttacac aggcagtaga ttgtgtgctg ccagatcggc ggcaatatct tccagcccca
    14221 gacgctgcaa tgtttcgcgg gtcggacaac caagctgtgg gtcccagccc atttctttgt
    14281 agaacatcgt gagcgaagca tgcatatcgt cacgatccat tttgtcagta ccttcagtaa
    14341 agaccgggat ctgcggatcc ttgtcgaata cccaggaaca gataagatcg tgttcattac
    14401 gcatatcttt ggtttgcatc agttttaccg tgtaggcacg atgcaacgta aagatacgct
    14461 ctgcagctaa atctaatttt tcctgagtca tctcttcgcc ggtgatcgct ttgaagaatt
    14521 tggcttcaag cgccagatcg ccccggtaat tacggctttt cagcggcgaa acggtcattg
    14581 gccagaccca gttgcacagg gtgacggcgt tatgcaaaca gacccgcaac agcgaccact
    14641 ttgcatattt aatttttgcg tcattgattg gcgtgtagtt tttggtttca tcgtaggcat
    14701 cttcagaacc aaaaagctct ttcgccactt cacgctgcag tttcaatggc aaacccgaac
    14761 caataaaatt gatatgggta tgcgtcatgc agtcacggtt gaacatacag ttaacaatgg
    14821 aaccgacctg cgctgacgct tcattggcat ggtgaaccgg atagccaaac ggcgaccaga
    14881 gtttattttt tgcgtagccc cagtactctt cacccaaatt ccagcgttct gcgatggcat
    14941 atgaaccatc agccaggtga ctcagctcac ccacacgatg cgccagacgg tagtaaaaat
    15001 ctttaatgaa attaacgtca cccgcttcca gttgatccca gtgaatttcc gcatactctt
    15061 cagctggcag aacacgcttg aacacccctt tgctgtaaca ataagtaaag tcgcgatgca
    15121 gctgcccgta gttacaccat aggccgtagt catcgaacaa attcagacca accaggttac
    15181 caatcacacg accatcgtct ttatcttcaa aatctttcgg cccgttcggg aagatggtgg
    15241 tatggacaaa gttggctaca caagtgttac cacctgtgct gggcacgcca aactctttca
    15301 cccgaggaat attcatttgg gtcatacaac ggatcgggca agagtggcag ccactcattt
    15361 ttactgtgta tttctctgcc gccggaccta agtcaaaaac ggatttatag gtacgaaagc
    15421 cgaccgtgtt ctgattgcct ggcggaattt cccccgtttc aatcggacca ccttcagccg
    15481 cgccccaaaa cagcccttta cgtgctgtcc aacgtgactt gggatctgaa tactctgccc
    15541 acgattgcgg cgtacttggc acgacatggt tgttattcgc accaataagt tcagtcatca
    15601 tgtaatcgtt gagacgcttc atctcctgac gatcggcaat gttgacccct ttcgtccctt
    15661 caaccgcaat cgctttcagg tttttcgaac ccataattgc gccagttccc gcaccgccgc
    15721 tgtggttacg gctattcaac atgccagaga gaggaacaag gttttcccca gcctgaccaa
    15781 tagccgccac acaggtttcc ggactggtca atcgacaaat ttcttccgtc gttgcgcgcg
    15841 tcccttttcc ccataagaaa tcggcttttt ccaggctaac tttgtcatct ttaatcttca
    15901 gccataccgg tgatttcgcc ttcccttcga taataatgac gtcgtatcca gcgaatttca
    15961 tttgcgctgc aaaaaagcca cccatatggg catcgacaac taaatttcct ttggtaaaag
    16021 tagaaagtga ggtgatattt acgcgagaac tacagggggc accagatcca gttaatgggc
    16081 cggtagcaaa gactaattta ttcgcttcat cgaaaggttt cgtgcctggc ggtacttcgt
    16141 catacataat tttgtagccg aagcccatgc caccgacaaa acttttaaac ttactggaat
    16201 cttcgagggt aatatttcct gtcgtgagat tgactcttaa tatattacct gtccaaccgt
    16261 tagccatgat tttttccttt gcaagattca cacagtaata tctttccact cgataatttt
    16321 taacgcccct gttgggcagg cgtttgcgca ttcaccgcat aacacacatt tcgaggattt
    16381 tttactttcg gtatttacgg tggccatcat ccacgggcat gccgtagtac aggcgctaca
    16441 gccaatacag cgtttatgat cgacggtaat acagccttct ttctgctgcc aggtaatcgc
    16501 accaatcggg cagacgttca tgcattgcgg ctctttgcat tgacggcagg tgtccgcggt
    16561 atagttgaga tcgccataca ggccgccgcc agagccaacc ccgttgtcgc caaagaaata
    16621 attgcggtgg attttgatac gggagaagaa tgtccctact gagccatcgt tgaagttggt
    16681 acaagagatt tcacagcggt ggcaacccgt gcatcgcgct cgctgggtca ccagcacccc
    16741 tttgggtgta tttatcaacc ctactgtgcc gctatcaata tcttcctgtt tgcaacccaa
    16801 aagtgacagt aacgcagggg caattgttag accagcaagg ccttttcctg atatacgcaa
    16861 gaattctaat cgcgttaagc caatatctaa tagtggacga tcaaccgggt tcatttattt
    16921 cgcatcctct tcgcaaacac agtgctgaca attcagcagc ctgatattaa gcattcaact
    16981 aatacgctgc tgactctgtc cccaacacca gatattttcc ctaacccgat agtgtatgag
    17041 aaaagattta acgccgataa actaatcctt tatggt
//
LOCUS       DAZGXZ010000109        16742 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000109 DAZGXZ010000000
VERSION     DAZGXZ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.109"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            99..1283
                     /gene="setC"
                     /locus_tag="ACHKJF_003804"
     CDS             99..1283
                     /gene="setC"
                     /locus_tag="ACHKJF_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFP0951076.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSSHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
     gene            1394..2317
                     /gene="yicL"
                     /locus_tag="ACHKJF_003805"
     CDS             1394..2317
                     /gene="yicL"
                     /locus_tag="ACHKJF_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP0951077.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(2321..3139)
                     /gene="nlpA"
                     /locus_tag="ACHKJF_003806"
     CDS             complement(2321..3139)
                     /gene="nlpA"
                     /locus_tag="ACHKJF_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP0951078.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            3361..3654
                     /gene="yicS"
                     /locus_tag="ACHKJF_003807"
     CDS             3361..3654
                     /gene="yicS"
                     /locus_tag="ACHKJF_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP0951079.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(3695..4885)
                     /gene="nepI"
                     /locus_tag="ACHKJF_003808"
     CDS             complement(3695..4885)
                     /gene="nepI"
                     /locus_tag="ACHKJF_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP0951080.1"
                     /translation="MSEFIAENRGADAINRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(5096..5548)
                     /gene="yicN"
                     /locus_tag="ACHKJF_003809"
     CDS             complement(5096..5548)
                     /gene="yicN"
                     /locus_tag="ACHKJF_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP0951081.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(5601..6935)
                     /gene="adeQ"
                     /locus_tag="ACHKJF_003810"
                     /gene_synonym="yicO"
     CDS             complement(5601..6935)
                     /gene="adeQ"
                     /locus_tag="ACHKJF_003810"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP0951082.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITRGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            7110..8876
                     /gene="adeD"
                     /locus_tag="ACHKJF_003811"
     CDS             7110..8876
                     /gene="adeD"
                     /locus_tag="ACHKJF_003811"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP0951083.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(8921..10312)
                     /gene="uhpT"
                     /locus_tag="ACHKJF_003812"
     CDS             complement(8921..10312)
                     /gene="uhpT"
                     /locus_tag="ACHKJF_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP0951084.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(10450..11769)
                     /gene="uhpC"
                     /locus_tag="ACHKJF_003813"
     CDS             complement(10450..11769)
                     /gene="uhpC"
                     /locus_tag="ACHKJF_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0951085.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(11779..13281)
                     /gene="uhpB"
                     /locus_tag="ACHKJF_003814"
     CDS             complement(11779..13281)
                     /gene="uhpB"
                     /locus_tag="ACHKJF_003814"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP0951086.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(13281..13871)
                     /gene="uhpA"
                     /locus_tag="ACHKJF_003815"
     CDS             complement(13281..13871)
                     /gene="uhpA"
                     /locus_tag="ACHKJF_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP0951087.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(13946..14236)
                     /gene="ilvN"
                     /locus_tag="ACHKJF_003816"
     CDS             complement(13946..14236)
                     /gene="ilvN"
                     /locus_tag="ACHKJF_003816"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0951088.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(14240..15928)
                     /gene="ilvB"
                     /locus_tag="ACHKJF_003817"
     CDS             complement(14240..15928)
                     /gene="ilvB"
                     /locus_tag="ACHKJF_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP0951089.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(16034..16132)
                     /gene="ivbL"
                     /locus_tag="ACHKJF_003818"
     CDS             complement(16034..16132)
                     /gene="ivbL"
                     /locus_tag="ACHKJF_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP0951090.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            16587..16700
                     /locus_tag="ACHKJF_003819"
                     /pseudo
     CDS             16587..16700
                     /locus_tag="ACHKJF_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009325922.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4280 a         4535 c         4124 g         3803 t
ORIGIN      
        1 gttgtatcag cgatactcaa aaattcttaa tacaatactc attcgactgg tacttatttg
       61 taactcagtt atattttttc gcccggtgat tcagaagaat gcaaaaaacg gctaccactc
      121 catcaaaaat acttgatctc actgccgcgg catttttact tgtcgccttt ctgacgggta
      181 ttgcgggcgc tcttcagact cctaccctaa gtatattcct cgcagatgaa ctgaaagccc
      241 gtcctataat ggtaggtttt ttcttcaccg gtagcgctat tatgggaatt ctggtcagtc
      301 aatttctggc aaggcactcc gataaacaag gcgaccgtaa attactgatt ctgctatgtt
      361 gcttatttgg agtgctggcc tgcacgcttt ttgcgtggaa tcgcaactac ttcattctcc
      421 tctcaacggg cgtacttctg agtagttttg cttccaccgc aaacccgcaa atgttcgccc
      481 tcgcccgtga acacgccgac agaacaggcc gtgagacggt catgttcagt acatttttac
      541 gtgctcagat ctcgcttgcc tgggttatcg ggccaccgct cgcttatgaa ctggcaatgg
      601 gatttagttt taaagtgatg tatctcaccg ctgccatcgc atttgttgtt tgcggactga
      661 tagtctggtt gtttttgcca tcaatacaaa gaaatattcc tgtcgttacc caacccgtag
      721 aaattttacc ctcctcccac aggaagcggg atacgcggct actttttgtg gtctgttcaa
      781 tgatgtgggc ggcgaataat ctctacatga taaatatgcc gctatttatt attgatgaac
      841 tgcatctaac cgataaactg gctggagaaa tgattggtat cgctgccggt ctggaaattc
      901 cgatgatgtt aatcgcaggc tattacatga aacgtattgg caagcgacta ttaatgctca
      961 ttgctatcgt gagtggaatg tgtttttacg ccagcgtact catggcgacg actccggcgg
     1021 ttgagctgga attgcaaatt cttaatgcca tcttccttgg tattctctgt ggtatcggca
     1081 tgctttattt tcaggacttg atgcctgaaa aaataggctc tgcgacaacg ttatatgcaa
     1141 atacttcacg cgtcggctgg attatcgccg gctctgttga cggaattatg gttgaaatct
     1201 ggagctacca tgcgttgttc tggctggcga tagggatgtt gggtattgcg atgatttgcc
     1261 tgctgtttat taaagatatt tagctcacca gtctgggcta agaaaaatcg gcgtggcgca
     1321 aacagagcca tcctcgccat ttttgctatc atgcctgcat acataaacga caaaacagta
     1381 tgcagaggga aaaatgggtt ccaccagaaa ggggatgctg aacgttctga ttgccgccgt
     1441 gttgtgggga agttcagggg tctgcgcgca atacatcatg gagcaaagcc agatgtcgtc
     1501 gcagtttttg actatgacgc gtttgatatt cgccggtttg attctactga cgctgtcatt
     1561 tgttcatggc gataaaatct tttctattat taacaatcat aaagatgcca ttagcctgct
     1621 gattttttcc gtggttggcg cgctaactgt acagctcact tttttgctaa ccatcgaaaa
     1681 atcgaacgca gccacggcaa cggtgctgca attcctctca ccgacgatta tcgtcgcctg
     1741 gttctcactg gtgcgtaaat cgcgcccggg cattctggtt ttctgcgcta ttttgacatc
     1801 gctggtcggg acttttttat tggtgacaca cggtaatccg acgtcattat cgatctctcc
     1861 tgccgcgttg ttctggggca ttgcctcggc atttgctgct gcattctata ccacctatcc
     1921 ctcaacgcta attgcccgct atggcacgtt accagtcgtc ggctggagta tgctgattgg
     1981 cggtctgatt ctgttgcctt tttatgccag acaaggaaca aactttgtcg ttaacggcag
     2041 tttgattctg gcgttttttt atttggtggt cattggtacg tccctgacat ttagtctgta
     2101 cctgaaagga gcacaattaa ttggcggtcc aaaagccagc attttgagct gtgcagaacc
     2161 attaagtagc gcgctactct ctttgctgtt gctggggatc acgttcacat taccggactg
     2221 gctgggaacg ctgctgattc tgtcatcggt gattttgatt tcaatggatt cccgtcgccg
     2281 cgccagaaaa ataaatcgtc cggcgcggca tgagtgagaa ttaccagcca ggcaccgcgc
     2341 caccgttaaa aatggtttct gccgctttag cgacttcggg tgattgataa gattgcagaa
     2401 attccttcac gttttctgca ttcttattat cttcccgtgc caccaaaata ttcacatacg
     2461 gcgaattctt atcttcaata aatacgctgt cgtgcaccgg agaaagccct gtctgctgaa
     2521 tgtaagtggt actgataatc gctacatcaa ctttgggatc atccagtacg cgcggaagct
     2581 gcgccccctc gagttccata atctgcaaat gacgaggatt atcggttata tccagcgcgg
     2641 taggtaataa cccttttccc tctttcagag taattagctt ctctttttgc aacagtaaaa
     2701 gtgcgcgacc aaggttggtc gggtcgttgg ggatcgccac tgtcgcgcct tcttttattt
     2761 gcgccacggt tgtgattttt ttggaataac ccgccatagg gaagacaaac gtatttccca
     2821 ccgctaccag tttataacca tgcgcctgat tatcttgttc aagaaagggt cgatgctgga
     2881 agacattcgc atcaagttcg ccatgatttg tcgcatcgtt cggtagcaac gaaccgctaa
     2941 atcctaccag ttcaacatcg aggccatatt tctcttttgc caccttttta gcgacttccg
     3001 cgacatcttg ttctgcgcca tttataacgc caactttaat gtgttttgca tcgctgctac
     3061 tctggtcgca acctgccagc agaattccgg ccagcaataa tgcggcccct gtccgtagat
     3121 gatgtgttgt cagtttcatt tttttatcct tttattaatg attacctgac tatagcggta
     3181 aggtcgctgc ggtttaaaaa acgaaacgct atcgataaga ataaaaagga ataaaagtgg
     3241 aatataaagt ctggcacttc ttactgacca cgcaagcgcg tttcgtacag catgatgaga
     3301 gcgatgaaag caaactacac ttatgcttca ttcgatacac atttgtaaaa ggttgaatcc
     3361 atgaaaccaa cgacactact gcttattttc acttttttcg ctatgccagg catcgtatac
     3421 gccgagtcgc cttttagctc actgcagtcc gcaaaagaga aaaccacggt attacaagat
     3481 ttacgtaaaa tttgcacgcc acaggcgtca ttatcagatg aagcgtggga aaagttaatg
     3541 ttgtcagatg aaaacaataa acaacatata cgcgaggcca tcgtggcgat ggaacgtaat
     3601 aatcagagca attattggga agcgttgggt aaggtagaat gcccggatat gtaagaacag
     3661 caacaactac tgaccaacgc ttctttacct gatatcagga tttcttcatt ttcacctttg
     3721 cagtaaccaa caatgcagtc agcaacatca atgtgccgga caacatcaac ggcgaagtca
     3781 gaccaatatt atccagcgca taaccgccga ttgccgcgcc acaggtatta gcaagctgaa
     3841 taaccgccac ctgaatagac ccggcttttt ctgcctgatc ggccagcgag cgggtgatcc
     3901 acgttgacca gccgacggga accaatgcaa aagttagccc ccagataatc gccacgccgg
     3961 tagcaacgat tttatcgctt ccccacaacg tcagcaccaa cgcactcaca gccagtatta
     4021 acggcgcgcc tgctaaggcc agttttaccg aacgtttaag aatgaatgac gaaagcgacg
     4081 taccgataaa gctggcaatg ccaaaactca acagcaccag cgttaagcca tccacgctga
     4141 atcccgccag gttcatatac accgggcgaa tatatgtgaa gaaagcaaac tgcccggcga
     4201 aagacatgaa gatggcgatc atccctgcca tcacacccgg acgttgtaat aagcggaaag
     4261 tattttgttt ctgatgcgag ggttcgcctg gcagtgaagg caatgatttg ataatccaga
     4321 aaatacacag cacgcccatc accgccgccg cattaaagac attgcgccaa ccgataagct
     4381 cgcctaaaaa actgcccaac ggcgcggcaa tcaccagcgc aatagaaacc gcgccgaaga
     4441 tcaccgacag cgccttcggc accgtacgcg gcggcaccag acgcatggtc agcgacgccg
     4501 acatcgccca gaacccgccc agcgccagcc ccagacaggc acgaccgatt aaaagcaaac
     4561 tgaatgagtt agcaaaggaa accagcaagc aggagagcgt cagcaaaacg gcaaacaaaa
     4621 taacaacgta gcggcggtca gtggcctgaa ttgtctgggt aataaacaaa ctggcaaaca
     4681 ttgccacaaa ggcggtcacg gtcaccgatt gcccggcaac cccttccgaa atgcctaaat
     4741 cctgggccat tggcgtcaac aaactgacgg gcaaaaactc aacgataatc agacaggcga
     4801 cacaaaacgc caccgagaaa acggctgacc agttcggtcg gttgatggca tccgcgccgc
     4861 ggttttcggc aataaattca ctcatggtgt tacccgtggc atgtttcagg ggaaaagccg
     4921 accactttaa cattgaaatg tgtgacgcat ttaacgtttt tgcaactttt acggtgttgc
     4981 ttgagctgga gttaagatcg aaacggaaca gggcaaagat taccggatgc ggcgtgaacg
     5041 tcttatccac ccgacaggtc aggcgcggtc tgtaggctga taagacgaaa cggtttcaat
     5101 gcaaccagtg aacgccctcg gcaggctgaa agaacgcgtt atagcgcatc tgaaaagcat
     5161 taatatcctg catttcaggt tcggtttcgc gcagaaaacc cagcgccagc ctgacctgag
     5221 cgtcggtaat cggcgcggcg aggcgagctt tttgtaaaat ccgatgccat tgcagcaggt
     5281 tctgttcgct accatcgaca atgacaattt gccagatgag caacacctgc gcggcgtttt
     5341 gcaggtgcga ctcatccggg cgatgcaaat aacgtaaaaa ttcgtcaccg gctgactttc
     5401 ccatttgatc gaccatcgac tccacgggta cgacatcgat attcagccga tcactgagac
     5461 ggcgaatcgc ttttctggcc ccggatgtca gcacccgaaa accaaccaca aacactaccg
     5521 ccagcgtggc gagcattatc cagatcatga gttctcctgc gtaatcagaa ctcatgatat
     5581 ctggaaaccc tcgccagtta ctaatccacc agaataatct tcagtgcaaa cagagctgcg
     5641 accaccacca cacacaggtt cagatcgcgc cagcgcccgg tgcacacttt catgatgcag
     5701 tacgacataa agccgagtgc aatcccttcg gtgatcgaga aagtaaaggg catcatcacc
     5761 gtggtaataa acgcaggcac cgattcggta aaatcatccc agttaacgcg cgccaggctc
     5821 gaagtcatca gcacgccaac aaagattaac gctccggcgg ttgcgtaagg aggaaccatc
     5881 gccaccagcg gtgagaagaa cataactaac aggaacataa cgccaaccac aaccgcagtc
     5941 agccccgtgc gaccaccgac tgccacacca gaagtacttt caatataggc agtaacagac
     6001 gaggtgccga taaacgcccc cgccaccgaa ctgacgctat caacatacag cgccttattc
     6061 atattgggga atttaccgtt actatctatt aagcccgctt tatcagttac accaattaat
     6121 gttcctgatg aatcaaatag gttgatcagc ataaaggaga aaatgatacc ggcgagttca
     6181 agtgttaacg cgccgctcaa atctacttca ccaatgacgc cgctaatatc aggcggaatg
     6241 gaatagacgc cgctaaaatg aacatcaccg aaaaataatc cacagcaaga cgtcaccaca
     6301 atagaaacca gcaccgcggc atgaaaatga cgtgatgaca acacggttat aataaaaaac
     6361 cctaaaatac ctaacaacac gccgtgagaa cttaaatcgc caatcatcac cagcgtgtct
     6421 ttattggcga caataacgcc agtatttttt aatcccatta aggcgataaa taatccaatt
     6481 cctctggtga taccaatacg taaacttaag ggaatgttgg agatcatcca gtaccggata
     6541 cgaaacagcg tgagcaaaaa tagtccaact gcgccccaga atattgcgcc catcccggtc
     6601 tgccaggaga tgcccatcgc ccccacgacc acgaaggcaa agaaggcgtt cagccccatt
     6661 gccggagcca gcgccacggg taagttagca aatatcccca tcgcaatact gccgataccg
     6721 gcaatcaaac aggtggtaac aaacaccact ttcgggtcca tttgtgccgc gccgaggatt
     6781 tgcgggttca caaaaacgat gtacaccatg gttaaaaaag tggtcatccc cgcaatcaat
     6841 tctgtgcgga cggtggtccc atgctgaggt agtttaaata atcgcgaaag cgtccctgat
     6901 tcattactca cgtaatcggt attgtcatta ttcatttttt tgtccattgg aggagattta
     6961 atcccttgct tttgtaaaac aaatgacaac aacggttcag tgataattat cacatttcaa
     7021 ttgcacatta atggatattc tttaataatc tcgcgacgtt tctttatgat aaataataat
     7081 caaattgata aaatcaaaat gagaaaaata tgaataattc tattaaccat aaatttcatc
     7141 acattagccg ggctgaatac caggaattgt tagccgtttc ccgtggcgac gctgttgccg
     7201 attatattat tgataatgtc tctattctcg acctgatcaa tggcggagaa atttccggcc
     7261 caattgtgat taaaggacgt tacattgccg gtgttggcgc agaatacgct gatgctccgg
     7321 ctttgcagcg gattgatgct cgcggcgcaa cggcggtgcc agggtttatt gatgctcacc
     7381 tgcatattga atccagcatg atgacgccgg tcacttttga aaccgctacc ctgccgcgcg
     7441 gcctgacgac cgttatttgc gaccctcatg aaatcgtcaa cgtgatggga gaagccggat
     7501 tcgcctggtt tgcccgctgt gccgaacagg caaggcaaaa ccagtactta caggtcagct
     7561 cttgcgtacc cgccctggaa ggctgcgatg ttaacggtgc cagttttacc cttgaacaga
     7621 tgctcgcctg gcgggaccat ccgcaggtta ccggccttgc agaaatgatg gactaccctg
     7681 gcgtaattag cgggcagaat gcgctgctcg ataaactgga tgcatttcgc cacctgacgc
     7741 tggacggtca ctgcccgggt ttgggtggta aagaacttaa cgcctatatt actgcgggta
     7801 ttgaaaactg ccacgaaagt tatcagctgg aagaaggacg ccggaaatta caactcggca
     7861 tgtcgttgat gatccgcgaa gggtccgctg cccgcaatct caacgcgctg gcaccgttga
     7921 tcaacgaatt taacagcccg caatgcatgc tctgtaccga tgaccgtaac ccgtgggaga
     7981 tcgcccatga aggacacatc gatgccttaa ttcgccgcct gatcgaacaa cacaatgtgc
     8041 cgctgcatgt ggcatatcgc gtcgccagct ggtcgacggc gcgccacttt ggtctgaatc
     8101 acctcggctt actggcaccc ggcaagcagg ccgatatcgt cctgttgagc gatgcgcgta
     8161 aggtcacggt gcagcaggta ctggtgaaag gcgagccgat tgatgcgcaa accttacagg
     8221 cggaagagtc ggcgagactg gcacaatccg ctccgccata tggcaacacc attgcccgcc
     8281 agccagtttc cgccagcgac tttgccctgc aatttacgcc cggaaaacgc tatcgggtca
     8341 ttgacgtcat ccataacgaa ttgattacgc actcccactc cagcgtctac agcgaaaatg
     8401 gttttgatcg cgatgatgtg agctttattg ccgtacttga gcgttacggg caacggctgg
     8461 ctccggcttg tggtttgctt ggcggctttg gactgaatga aggtgcgctg gctgcgacgg
     8521 tcagccatga cagccataat attgtggtga tcggtcgcag tgccgaagag atggcgctgg
     8581 cggtcaatca ggtgattcag gatggcggcg ggctgtgcgt ggtacgtaac ggccaggtcc
     8641 aaagtcatct gccgttgccc attgccggcc tgatgagcac cgacacggcg cagtcgctgg
     8701 cggaacaaat tgacgccttg aaagccgccg cccgtgaatg cggtccgtta cccgatgagc
     8761 cgtttattca gatggcgttt ctttctctgc cagtgatccc cgcgctaaaa ctaaccagtc
     8821 aggggctatt tgatggcgag aagtttgcct tcactacgct ggaagtcacg gaataataaa
     8881 aaagcccggc gtcatgccgg gcaaaagtca ccagttacgt ttatgccact gtcaactgct
     8941 gaattttttt ctcgcggcgg attttgcgtt cttccatcac cgccactatc gccatcaggc
     9001 agatacaacc aatcgcggcg atatcaagtg cggcgaatgt gcctgcccag ccggtaaggc
     9061 cgaataccgg cgtcccatcg gcaatcattc ccagacctaa cttggcaaag ctgtcaccaa
     9121 tcaggtaagc aaaggtgcct ttaataccat cggcagcgcc aatcgctttt ttaggtacaa
     9181 agccaacagc agccacacca atcaacaatt gcgggccaaa gaccaggaaa cccaacgcaa
     9241 agagagaagc cagatagata tattcgttgc tggcatgttg atacacaccg agcgtggcga
     9301 taatcagcgc cagcgcgatg caggccacca ggccacggcg accgttcgcc aggtcagaga
     9361 gccagcccca cagcagcgta ccgaccagcg caccagcttc aaacagcgta aagccctgaa
     9421 tcgccaccgc tttagagagt ttcagttcct ggaacgcgta tacggttgac cactggtcga
     9481 taccaatacg taccacatag aggaaaatgt tggcgaagca cagcagccag atcactttgt
     9541 ttttcagcac atactcaaca aagatctgcc acttggtcat atcggtagat tctgtctctt
     9601 tgtcctcttc gctgatctcc tcgccgaaca gttcttcagc tttgccgagg ccataagatt
     9661 ccggggagtc gctgccgtaa cgcaggccga taaaaccgac aatcagcgcg ataatcgacg
     9721 ggaagataaa catgccgatg acatggccat cgaacaggta atttgccccg aacagcgcca
     9781 cacctgctgc gcctgcaccg ccaaggttgt gagaaatatt ccagaaaccg aggaatgtcc
     9841 cgcgtttacg acgcggcgtc catttggtga tggtggagta actgcacgaa ccgccggtac
     9901 tctggaaaaa gccgcttaag gcgtagaagg caatcatcag gaacaggcta accgagccgc
     9961 tgcccatact ggcactgaag cccagcatac aaatggcaga caggatcagc atgaacggca
    10021 ggaattgttt ggtgtttttg ccgtcggcgt agtaggaaac cagcgtttta cccacgccat
    10081 aagtgatgga gaaacccagg ccgatcatcc ccagctgcgt catgctcaac ccgtaggtcg
    10141 agatcatgtc gttctgcgcg atgttaaagt tcttgcgaat caggtacatc gtcaggtagc
    10201 cgataaagac caccaggtag gattgcatga acggtttgaa ccacattttg cgccgcactt
    10261 cgagcggaag gtccagggtc ggcttgcgaa cctggtttaa gaaagccagc atgggttact
    10321 cctgaaatga atacctgcgt gaggcatgca ttgtaaaaat cagcagcaaa tcaggcctga
    10381 gatagcgtcc aggcaaaacc tggaaaattt cttagttttg ccggatatga agtgaaaagg
    10441 tgagatgcat cacgcttcgc gcggtgcctg ggcgttcaaa aagggcagta acagcagtgc
    10501 ggaaatcccg gcggcgatag agataaccac aaaaaatccg ctccagtgcc aggtatcgag
    10561 tactttcgcc agcggccaac cagcaagcga cgcccccaga taagcaaaca agccgacaaa
    10621 ccccgtcgcc gcccctgccg cctctttgtg ggaacactct gccgccgcca tgccgattaa
    10681 catctgcggg ccaaagacaa aaaaaccaat ggtgaagaag caggttgcct gcatcacgta
    10741 gctggcaaat ggcatcagcc acagcgagcc gactgaaagc aaaattccgg cggcgaaaat
    10801 caaattcatc ggcccacggt tgccgttaaa caatttgtcc gagccccaac cggctaccag
    10861 cgcaccgata aatccgccca gttcaaacat cgtcactgcc gtattcgccg tgaccagatc
    10921 gacgcccagt gtctcggaca tatacaaatt gccccagtcg ttgatcgccg cccggaccac
    10981 atagaccagc acatagcaaa acgaaagcag ccagatatac ggattcagca acacatattt
    11041 ggtgaggatc tctttacgcg tcaaccctgc cccttcttgt tgttgagcaa tttccagcgc
    11101 gtcgtgtcgc cattcaccga ccgccggtaa acctaacgcc tgcgggcgat cgcgtagccg
    11161 ccagcagaga aaaatcccca cgactatcgc catacaacca gcaatcatca tcccggcacg
    11221 ccagccgtaa tgcagcgcag ccgctgccat cacaatggga atgagtgcgc cgccgacgtt
    11281 atgcgccgtg ttccataatg cccaccaacc gccgcgctcg gtacgtgaat accaggccgt
    11341 taacagacgc gcacacaccg gtgaacccca gccctggaaa aaggcgttca gcacccagag
    11401 cacggcaaac gcccatagcg acgtcgagaa gccaaacaga atgttgatga tgcccgtggc
    11461 gataagccct atccccataa aataacgggc atttgagcga tcgctgacaa tgccggagac
    11521 aaacttcgac acgccgtagg taatgtaaaa cagggtcgct aacaggccga tatcgctacg
    11581 gctgagcacg ccgttagcaa ggatttctgg tacggcggcg ttaaaacttt tacgcgtgaa
    11641 gtaaaacagc gcgtaaccca gccagatggt cagcagaata tgccgacgcc agtagcgata
    11701 gcgggcatca atttcatgtt tgtcagtcat taatggcgca tcggcaggcg ctttcagaaa
    11761 cggcaacatc atcgaacctt agacatagcg ttgaggtaga gaaacgctga cacgcgtgcc
    11821 gtgcagacag gaaatgtgta atgtgccgcc cagcgccgtt acgcgctcgc gcattccggt
    11881 gaggccaaaa ccttgttgcc cggaacccgg cggcaaaccg ctgccatcgt cttcaatcac
    11941 cagcatcaac cgttcatcct gctgccagcc ttgcagcgtg accgcgctgg catcagcatg
    12001 tttcacaatg ttgttcagcc cttcctggca gacacgaaac agcgtcacgc gctggttttc
    12061 acttaatgct gattcatcga ttcgccattc gagatggctg acaatgccgc gcccttccag
    12121 ctccatttcc cgcatcagtg agcggatggc ctgctccagg gagagatcat ccaactggcg
    12181 cggacgtaac cgtcctaata aacggcgcac cgcgtcgtac acgcccagcg acagttgttc
    12241 gatgagctgc ccgctctgct tcacgctggc gttatctgcc gccagccgct gaacaatgcc
    12301 cgcctgagta cgaatggctg tgatggtctg accgatatca tcatgcagct cacgcgccac
    12361 atcacggcgc acgctctctt cggtttccag caaccgttca gccagatgct gattgcgcgc
    12421 cagttccttt tgcagcgact ggttaagttc acgcaaccgc tggatgccag cgccaagcaa
    12481 caaccctgtc agactttgca ccagcagcga gagcaataaa tccaccggat gatcgcgcca
    12541 ggtttgactg gcgatcagcg cgatggcgtt catcaacgtc gcaatcagcg ccccttgcca
    12601 accatagtgc caggccagcg cgataatcgg cagcgccaga caaaatggcg taaagcgcga
    12661 cagttcgtcc ggcaatccca actggagcca gagactgata acaaacagca gcaagtacca
    12721 gaccagatgt cgcccgcgcc agttgatcgg ctgagaaacc agtgacgggc cgagcggcag
    12781 ccaggtgtta ttggcgagat agtgccagaa caccagacat atcggggcca gcgtcaggcc
    12841 gccagttaaa gtcagcaaca gcgcattcca cgactctttg ccgtgccaaa gccagggcag
    12901 cgactgcaac aacgccgccg ccgttaacgc cgcaccctgt aacagcaagg tgcgccagtc
    12961 acgctgatgg cgatagcgcg agatcagcgc cacgggcagt aacgtcagta aactaccgat
    13021 catcaataac ggaaaatggg ttaaaccgac cgcctgcgtt agccagtaaa tcagcagcca
    13081 ctccgcgccc agcagcaccg gccagtatcc gcgcgggcat tgcagcatta gccccagacg
    13141 cagaccaaac ggaaataaca gcaccgccat atcagggcgc tcaaccaggt gcaggctgat
    13201 actccacagg caaaaccatg cggcagagaa gataaaaaag caggcaataa cggtaattaa
    13261 gcgggagaac aacgtcttca tcaccagcca tcaaacatgc ggcgcgccag ctctacgtcg
    13321 ttactgacac ccagtttttc catcagattg gcgcgatgga cgtgtaccgt tttcggtgac
    13381 aagcccagtt cggcggcaat ctctttcacc gccattcctt gcgccagttt ttccgccacc
    13441 tggcgttcac gtttggttag cgggtcctga cgaccggatg ccagtttaat ggcaatatcc
    13501 ggcgtcagat aacagccgcc cgtggcaacc gtatgcaccg cagcaatgag ttcatccggg
    13561 ctacagcgtt tggaaagaaa gccgcgtgcc cccgcgttaa gcgcctgctc aaccagcgca
    13621 ggactgtcgt gaacggagag cataatcgtc gccatacctt tcggcagctg gcttagcagc
    13681 tccagaccgg agatatcggg catggagata tcgcaaatac acacctgcac accgcgcccc
    13741 ggcagccccg ccagcgcctc gcgccccgaa ccaaactcgg caactacctg caaatcaggt
    13801 tccagcccca gcagctgcgc aaagccggag cggacgatga ggtgatcgtc tataagggca
    13861 acggtgatca tggtcttgtc ctggcgggta aaaaaaaacg cgcttacctt aacgataagc
    13921 gcgatgttgt tcaagccttg agcggttact gaaaaaacac cgcaatcttg ttaaacatag
    13981 tcggatcgga ctgattacgc tgcactttca ccacatcttc cagcttatcg atttggctta
    14041 tcatctgctc cagacgctgg tcgtcattga ccagtagcca gatgtggctt ttgtcgctgt
    14101 cctgaatcgg caggcagaga atgccttcaa cgttaaaagc gcgacgggca aaaaggccac
    14161 aaacgtgggt cattacgccc ggatggttgc gaacggtgag ttccagaatc acgttgtcat
    14221 gagttgtgtt ttgcatggct tattccccca ccatttcagt attcgccgca ccaggcggca
    14281 ccatcgggta aactttttct tcggcatcaa tgcgcacatg gatcagcgcc ggaccagggc
    14341 gattgatgat ttcctgcaat gcagcctgcg gatcggcttc gttattcaaa tcacaggttt
    14401 cgaggccgaa tccggcggca atctgcataa agttgatttt gcccggatag gtggcggcaa
    14461 aaacgccctg ctcgtagaac agactctgtt gctgatgtac cagccccagc gcttcgttgt
    14521 tcatcaggat gattttgaca tccagctgat tttcactggc ggtcgccatc tcctgaatat
    14581 tcatcatcag gctgccgtcg ccggagaaac acaacacttt gcgatccggg ttcgccagcg
    14641 ccgcgccaat cgccgcaggc aggccaaaac ccatcgtgcc cagcccaccg gaggtcagcc
    14701 actggcgtgg gcgattgagc ggataagctt gcgcggtcca catctgatgc tgacccacat
    14761 cggtggtgat aatcgcattg tcatcgacac aggcggcaac ggcgttgatc aggccgtaat
    14821 ggcttaatgg atcgcacgct ttcgggattg gacacgggaa ttcgcgctgc aaatccgcta
    14881 ccaactggtg ccactctgca cgcggttgcg cttccacctg cgggatcaat tgcgccagca
    14941 cgtcatcaac atccgcctga atcgccacat gcggctgctt gattttaccc agctctgcgc
    15001 gatcgatatc gacatgaata attttggcat tcggacagaa ctgctcggtt ttgccaatcg
    15061 cccggtcatc aaaacgcgca ccgagcacta tcaacaaatc cgcctcctgc aaaatatagt
    15121 tggtgctgcg cacgccgtgc atccccagca tacccagcga caacggatgc gcttttggca
    15181 acatgcccag cgccattaaa gtcatggtgg taggcagttg cgctttctcc gccagttcac
    15241 gcacccgtgc gggcgcattg atcacaccgc cgcccagata aagcaccggg cgtttggcag
    15301 cgttaatcat cgccgctgcg tcacgaatgc tttcttcgct aaaggcgggg gcggcggctt
    15361 tttctgccat agcgggctgt gtttcaatct caaaaactgc cgtttgcaca tccttaggaa
    15421 tgtctatcca caccgggcct gggcggcctg attgcgcaat gcggaaggca tcgctcatga
    15481 cctgcgggag ttcttcgata tgtctgacca gatagttgtg tttggtgatg gggatagaga
    15541 tgccgtaggt gtccacttcc tggaaggcgt cggtgccgat catcgaggcg ggaacctgac
    15601 cggtgatgca aatcagcggg atggagtcca gccgcgcatc ggcaatggcg gtcaccaggt
    15661 tagtcgcacc cggtccgcta caggccatac agaccgccgg tttaccgtcg gtgcgcgcca
    15721 tcccctgagc gataaatccc gcgccctgtt catgacgggc cagaatatgg cggatttgcg
    15781 tgctttggct taaggcatcg taaacaggca ggatagaacc gcccggaatg cccgtcacaa
    15841 tcttaatgcc ctgctgttcc aggaaatgaa cgataaattc tgcgccggta aagcgcttac
    15901 gcgtcgatgt tgtgcccgaa cttgccatgc tccagtcctt ttcttctggg ccgactttcc
    15961 gggaggtgct taaacgaaaa accccgcccg gtttgcgccg gcggggtttt ggaatcgtgt
    16021 gttgttccag tccctacggc gcattgccga cgaccaccac cacacgcacg acgaccactg
    16081 cggcggatag cgcagttggt agtagttttg cgttgaacat ggaagtagtc attggggacc
    16141 ttgttggttt tgtgtttaac aatatttata caagcacagc tttacagggg agacaatgga
    16201 aaatttttca gcaagggaaa attgaggggc tgatcacgtt ttgtactgaa ttgcaggtaa
    16261 caaaaaaccc cgccggagcg aggtttcatc agccgcctgc ggctggtaac cgcaaagcac
    16321 actgtattat gtcaacactg aaagtatacg tgttccgcgc agaacgcgca atttcggcac
    16381 gaattttgac gtatttaggg catagttgag tatcgatcac agtttgcgtt ttgtccaaat
    16441 attactgttt atttatacag taaacttcta taatatcact gtacgcaatg tgttatgcgg
    16501 gggccgcatc gttacccggc gcactaagtc ctggctgaaa cgggtggtgc cgtcagcgcc
    16561 ttaaccccgc gtgagcacac tgtgttatgt caacaagcac aacgtttctc cttaagatac
    16621 cgcgtgcaca acagctggca acaggcagcg gaaaggtacg tcagctggca gtgctcctga
    16681 accacaggag acgcgtatga gcctggtgga tatcgccatt cttatcctca aactcattgt
    16741 tt
//
LOCUS       DAZGXZ010000110        16605 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000110 DAZGXZ010000000
VERSION     DAZGXZ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.110"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(140..292)
                     /locus_tag="ACHKJF_003820"
     CDS             complement(140..292)
                     /locus_tag="ACHKJF_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0951091.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(557..1291)
                     /gene="cysH"
                     /locus_tag="ACHKJF_003821"
     CDS             complement(557..1291)
                     /gene="cysH"
                     /locus_tag="ACHKJF_003821"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0951092.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1365..3077)
                     /gene="cysI"
                     /locus_tag="ACHKJF_003822"
     CDS             complement(1365..3077)
                     /gene="cysI"
                     /locus_tag="ACHKJF_003822"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP0951093.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3077..4876)
                     /gene="cysJ"
                     /locus_tag="ACHKJF_003823"
     CDS             complement(3077..4876)
                     /gene="cysJ"
                     /locus_tag="ACHKJF_003823"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP0951094.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5195..5557
                     /gene="queD"
                     /locus_tag="ACHKJF_003824"
     CDS             5195..5557
                     /gene="queD"
                     /locus_tag="ACHKJF_003824"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP0951095.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5635..6906
                     /gene="ygcN"
                     /locus_tag="ACHKJF_003825"
     CDS             5635..6906
                     /gene="ygcN"
                     /locus_tag="ACHKJF_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0951096.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6897..7157
                     /gene="ygcO"
                     /locus_tag="ACHKJF_003826"
     CDS             6897..7157
                     /gene="ygcO"
                     /locus_tag="ACHKJF_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0951097.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7174..7749
                     /gene="ygcP"
                     /locus_tag="ACHKJF_003827"
     CDS             7174..7749
                     /gene="ygcP"
                     /locus_tag="ACHKJF_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP0951098.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7897..8757)
                     /gene="ygcQ"
                     /locus_tag="ACHKJF_003828"
     CDS             complement(7897..8757)
                     /gene="ygcQ"
                     /locus_tag="ACHKJF_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0951099.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8754..9533)
                     /gene="ygcR"
                     /locus_tag="ACHKJF_003829"
     CDS             complement(8754..9533)
                     /gene="ygcR"
                     /locus_tag="ACHKJF_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP0951100.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9511..10920)
                     /gene="ygcS"
                     /locus_tag="ACHKJF_003830"
     CDS             complement(9511..10920)
                     /gene="ygcS"
                     /locus_tag="ACHKJF_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951101.1"
                     /translation="MTWRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10942..12396)
                     /gene="ygcU"
                     /locus_tag="ACHKJF_003831"
     CDS             complement(10942..12396)
                     /gene="ygcU"
                     /locus_tag="ACHKJF_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP0951102.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12466..13251)
                     /gene="ygcW"
                     /locus_tag="ACHKJF_003832"
     CDS             complement(12466..13251)
                     /gene="ygcW"
                     /locus_tag="ACHKJF_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0951103.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13570..14847
                     /gene="yqcE"
                     /locus_tag="ACHKJF_003833"
     CDS             13570..14847
                     /gene="yqcE"
                     /locus_tag="ACHKJF_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP0951104.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14874..16352
                     /gene="ygcE"
                     /locus_tag="ACHKJF_003834"
     CDS             14874..16352
                     /gene="ygcE"
                     /locus_tag="ACHKJF_003834"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP0951105.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3976 a         4351 c         4170 g         4108 t
ORIGIN      
        1 taaattaatg attttcattt gaaattcata ataataaata gagactatat attcacataa
       61 aaaatataaa ttttaaaaaa gggtgcagga gaccaaacca tcacaattca gtacgcgggg
      121 ttttaccccc gcgtggtggc tacttcttcg attcgtaagc gagaactgcc ttaaactcca
      181 taccacgttc tctgatactc aactcacaca acgagtcgcc taccatcagc gtgaatccca
      241 gtactgtaaa acacagcact atgattgcca caagggcata ttttgtcagc atgtcttgcc
      301 tccctgcaaa gaagagacta acattcgaat tgtttgggtt cgaacgctgg cctcaggttg
      361 atagaaatat cgcctggggc ttttgtccat ctggaacctc gcgaatgctt aacgccagac
      421 agcctcaagc acccgacgcc attctatacc tgataattct tccagcggcg ttcacgttta
      481 aaatcaggaa tactgctcgc aaaataaaaa cgcccccatc atttatgaca gaggcgttta
      541 atttatccgg caatatttac ccttcgtgca atccacattc cctttttaag ccaaagaaac
      601 gtgtttcttc ttccgacatg ccaggttccc atttacgggt tgtatgggta tcaccgaccg
      661 ataaatatcc ttcatcccat aatgggtgat atttcaggcc atgtttttgc agatactgat
      721 atatcgttcg gttatcccag tcgataatcg gcagcacttt aaatacgcca cgctgaattg
      781 ccagcaccgg taaattggcg cgactgccgg attgttcacg gcgcaggcca gcaaaccagg
      841 tttgcgcgtt aagttctttc agagcccggt tcatcggttc gactttgttg atgtcattgt
      901 acttttcaat gccttcaacg ccctgctccc acagttttcc gtagcgtgct tcctgccagg
      961 ctgcgctttc ggtagcacgg tacactttca ggttgagctt gagtttgtcc gttaactcgt
     1021 caataaagcg gtaggtttcc gggaacagat agccggtgtc ggtgaggatc accggaatat
     1081 ccgggtgaat ctgattcacc agatgcaggc tcaccgccgc ctgaatgcca aagctggaag
     1141 aaagtacata ttcaccgggc agattatcca gcgcccaggc aacgcggcct tcagcgtcca
     1201 gtttttccag ttcggcgtta gtttctgcca gcgccagaat gcgatctacc ttcggcagtt
     1261 cgttcagggc gtttagatcg agtttggaca tagattcctc actgtttgcc ttgcctgatg
     1321 cgacgctcac gcgtcttatc aggcctacaa gaccgggctg atggttaatc ccacaaatcg
     1381 cgcgccggat cgagcaccgg gcgaatgatg cccgcacgca ccgtaaagtc gccgaagcct
     1441 tcacccgctt cgcgctcttt cgcccagcgc cctatcagtt catcaagcga cgccaggatt
     1501 tccggctcgg tgatgttttc tttatacatc cgtgggatac gtgtcccaat gcggttgccg
     1561 ccaagatgca gattgtagcg acccggtgct ttacccacca ggcccacttc cgccagcatc
     1621 gcgcgaccac aaccgttcgg gcagcctgtt acacgcatca cgatatgctc atcgctgaca
     1681 ccatgtttcg ccattaaatt atcgatgttg tcgataaaag acggcaggaa acgctccgct
     1741 tccgccatcg ccagcgggca agtcgggaat gacacgcagg ccatcgagtt ttcacgctgc
     1801 ggcgtgacgg cattcattaa accgctctct ttggcgatct tctcgatctt cgctttctcg
     1861 ctttccggta caccggcgat gatcagattc tggttcgccg taatgcggaa atcgcctttg
     1921 tggatcttcg cgatctccag caggccggtt ttcagcggac gccccggata atcaaggatg
     1981 cgaccatttt cgataaacag cgtcaggtgc cagttatcat caatgccctt aacccagcca
     2041 atacgatcgc ctcgtccggt gaactcatat ggacggatcg gttcaaattt gatccccgcg
     2101 cgacgctcca cttccgcttt aaacgtctca acccccacgc gctccagcgt gtatttggtt
     2161 ttggcatttt tacgatcggt tcggttaccc cagtcacgct gagttgtcac gacggcttcg
     2221 gccaccgcca gcgtatgctc cagcggcaga tagccaaact cgctcgccgt gcgggcgtag
     2281 gttttcttgt tgccgtgttc gatagaaagc ccaccgccca ccagcaggtt gaagcctacc
     2341 agcttgccgt tttcggcgat tgccacgaag ttcatgtcgt tggcatgcag atcgatatcg
     2401 ttctgcggcg ggatcactac cgtggttttg aacttacgcg gcaggtaggt ctgaccaagg
     2461 atcggttctt catcagtagt ggcgactttt tcctggtcga gccagatctc cgcatacgcg
     2521 cgggtacgag gcaacagatg ctcagaaatc ttcttcgccc actcgtacgc ttccgcgtgc
     2581 agctgcgact cgtaagggtt cgaggtgcag agtacgttac ggttcatgtc gttagctgtc
     2641 gccagcgcat caagaccgac cgagtgcagc atctggtgca ccggtttgac gttctttttc
     2701 agaatgccgt ggaactgaaa cgtctggcgg ttggtcaggc gaatgctgcc atagatggtg
     2761 ttttcaccgg caaatttgtc gatcgcctgc cactgtttag tggtaatcac cccacccggc
     2821 agacgacagc gaagcagcat cgcgtggcgc ggctccagct tctgttcagc acgttcggcg
     2881 cggatgtcgc ggtcatcctg ctgatacatg ccgtggaagc ggatcagcag gaagttatcg
     2941 cccttaaagc cgccggtcag accgtcgttt aaatcttccg caatggtgcc gcgcaggtag
     3001 ttgctttcaa gcttcatgcg ctcggcgtct gtcagttttc cttcgaccac taaaggccct
     3061 ggatgttttt cgctcattag tagacatctc gctgataacg gcgctctacg cgcagctcac
     3121 ttaaaaattc atccgccgct tcggtgtcca tgccaccaaa ttcggcaatc acttccagaa
     3181 gtgcctgctc aacgtctttc gccatgcgat tagcgtcgcc gcagacataa atgtgggcac
     3241 catcattgat ccagcgccac agctccgcgc cctgttcgcg cagtttgtct tgtacgtaaa
     3301 ctttttcttt ttggtcgcgc gaccaggcaa gatcgatacg tgtcagcacg ccatctttga
     3361 cgtagcgctg ccactccacc tggtacagga agtcttccgt aaagtgcgga ttaccaaaga
     3421 acagccagtt tttacctggc gcttcgtcgg cggcgcgttg ctgcataaag gcgcggaacg
     3481 gcgcaatacc ggtgcctggg ccaatcataa tcaccggggt ttctggattg gctggtaggc
     3541 ggaagttatc gttatgttcg ataaatacgc ggacttcgcc ctcttcttcc acgcggtcag
     3601 cgaggaagct ggaggcacca ccggcacggg cgcggccttc cacgtcgtaa cgcaccacac
     3661 caacggtgac gtgtacttcg ttctcgactt ccgcctgcga ggaggcaatg gaatagagac
     3721 gcggcgtcag cgggcgcagc agattaatta gcgcttcggc atcaagctgt gccggggaga
     3781 aacgcaccat gtcgacaatc ggcgtcgtcg cggcgtaatg ctgtaacttc gctttatcgc
     3841 ccaccagcgg cagcagtgtt tcgctgcggg taagcgtggc gtaattctca acaatgttgg
     3901 cggtgttgac ggtcagttcg aagtgccact gtagcgcttc gttcagaggc aacgttttgc
     3961 cctcgacggt gacaggttca tcgcctttca gccacagcag ttcgacaagt tctttcacca
     4021 gtgccggatc gttctgatac cagacgccca gcgcgtcacc cggctggtaa cgcaggcccg
     4081 agtcacctaa gtcaatttcg atatggcgaa cgtctttttc agagttacgc ccggtaattt
     4141 tctggttaac agagaggcta gccaccagcg gcgcgtcttt gctgtacggg ctggtgtgga
     4201 tttcatttac cgcgccagta gcgacggatt gcgaaggtgc cgcgacaggc gcacgcgatt
     4261 taagcgcatc aaccacgcgg gcgcgccact cgctggcagc agcctggtat tcaacatcgg
     4321 catcgacacg gtcgagcagg cgttcaccac ccagttccgc cagcttgctg tcaaaatctt
     4381 tcccggactg gcagaaaaat tcataagagc tatcgccgag gctaaacacg gcaaatgcgg
     4441 tgttttccag cttcggcgct tttttggaga acaggaactt atgcagcgcg acggcttctt
     4501 ccggcggttc cccttctcct tgcgttgacg tcaccacgat aagcagtttt tcgctggcga
     4561 tttgtttgaa tttatagtcg cccgcgttca ccagcttaac gttcagtttt gccgctaata
     4621 aatcatcacg taatgcttca gcaacacggc gcgcattgcc ggtttgcgag gcggagataa
     4681 tagttatacc cggcatttct gcggcgggag ctggcgtcgc tgcaagcgca gcaggctgct
     4741 gattgagcac gccccagaaa tagccagaaa cccaggcaag ctgggtggga gttaaatcgg
     4801 tcgtggccgc ctgaaggcgt accagttgct ccgggttcaa cggaagcaac gcggaaggtg
     4861 ggacctgtgt cgtcatgcgt cgttatgttc cagtaagcaa agctgtttct gcgccctgtc
     4921 agcgcccata aaacagaaga gaaggtaagg ttaacggggt gaacggtgtg gattaaagac
     4981 gggatagcga taactaataa ccaaatcgac taacctgttt tagcaatagt ctttaacaac
     5041 aaaatagatt aaccaaccta atgaaaaaca aatgaattta gccaatcatt aagataaatc
     5101 agcgattttg cgcaacaaag tcgttttaga taatgcgaaa aaacagcctt tccggtactc
     5161 tacggcggtt ttatcgtcct gtagagaaat tatgatgtcc accacgttat ttaaagattt
     5221 caccttcgaa gccgctcacc gcttaccaca cgtcccggaa gggcataaat gtggtcgcct
     5281 gcacgggcat tcctttatgg tgcgactgga aattaccggg gaagtcgatc cgcatacggg
     5341 ctggattatc gatttcgctg aactgaaagc agcgtttaag ccaacttatg agcgcctcga
     5401 tcaccattat ctcaatgata ttccaggcct ggaaaacccg accagcgaag ttttagcaaa
     5461 atggatttgg gatcaggtta aacccgttgt gccgctgtta agtgcggtga tggtaaaaga
     5521 aacctgcacc gctggttgta tctatcgcgg cgaatgataa gagtgtgtcg gcggtcaatt
     5581 tcccttaagt aacgctatgt tagggtgttg tgttctggat atctggggca tgacatggaa
     5641 gacgactgcg acattattat tattggtgcc ggtattgcag gcaccgcttg cgcgttacgc
     5701 tgcgcgcgag cgggtttatc cgttttgtta ctggaacgcg ctgaaatccc cggcagcaaa
     5761 aatctttccg gcgggcggtt atatacccat gcactcgcgg aactcctccc tcagtttcat
     5821 ctgaccgcgc ctcttgaacg atgcatcact cacgaaagcc tttccctgtt aacgccggat
     5881 ggcgcaacga cgttttccag cttacagccc ggcggtgaat cctggagtgt attacgtgca
     5941 cggttcgatc cgtggctggt tgccgaagcc gaaaaagaag gtgtcgaatg catccctggt
     6001 gcgacggtgg atgcactgta tgaagaaaac ggcagggtgt gtggtgtcat ttgtggtgac
     6061 gatattctcc gcgcccgtta tgtggtgctg gcagaaggtg ccaacagcgt cctggctgag
     6121 cgtcacgggt tagtgactcg tcctgctggc gaagcgatgg cgttggggat caaagaagtg
     6181 ctgtcgctgg aaacatccgc tattgaagaa cgttttcatc tggagaataa cgaaggcgca
     6241 gcgttgctgt tcagcggcgg gatctgtgat gacttacccg gcggcgcatt tctttatact
     6301 aatcaacaaa cgctctcgtt agggattgtt tgcccgctct cttcccttac gcaaagccgt
     6361 gttccggcaa gcgagctgct gactcgcttt aaagcgcatc cggcagtgcg cccgcttatc
     6421 aaaaacacgg aatcactgga gtatggtgcg catctggtgc cagaaggtgg cttgcacagt
     6481 atgccggtgc aatacgccgg taacggctgg ctgctggtgg gcgatgcgtt gcgcagttgc
     6541 gtcaataccg gaatttccgt gcgcggcatg gatatggcgc tgactggcgc gcaggcggcg
     6601 gcacaaacgc tgataagcgc ctgccagcac cgcgagccgc aaaatctgtt tccgctttat
     6661 catcacaacg tagagcgcag cctgctgtgg gatgttctac agcgttatca gcatgttccg
     6721 gcgcttttgc aacgcccggg atggtaccgt acgtggcctg cgttaatgca ggatatttcc
     6781 cgcgatttat gggatcaggg tgataaacct gttccaccgc tgcgccagtt attctggcat
     6841 catttacgtc gtcacggcct gtggcatctg gcgggcgatg ttatcaggag tctgcgatgt
     6901 ctgtagcccg taatctctgg cgcgttgctg atgcgccgca cattgttccg gctgactccg
     6961 ttgagcgcca gacggcagaa cggttgatta gcgcctgtcc ggcaggtctt ttttcgctca
     7021 caccggaagg tgacttacgt attgactatc gcagttgcct ggagtgtggc acctgccgtt
     7081 tgctgtgcga cgaatcaaca ctacaacagt ggcgctatcc gccttccgga ttcggcatca
     7141 cctaccgctt tggataaata ataaggataa tttatgcccc tcttacacct gctccgccag
     7201 aatccggtga ttgctgccgt taaagataat gccagcctgc aactggcaat cgattctgaa
     7261 tgccaattta tttccgtgtt gtacggcaat atctgcacca tcagtaatat cgttaaaaag
     7321 attaagaacg ccgggaaata tgcttttatt catgttgatt tgctggaagg cgcgtcaaat
     7381 aaagaagtcg ttattcagtt tttgaaactg gtgaccgagg cggacggcat tatcagtacc
     7441 aaagcctcaa tgttgaaagc ggcaagagcg gaaggttttt tctgtattca tcgcctgttt
     7501 attgttgatt caatttcgtt tcacaacatt gataagcaag ttgcgcaatc gaatccggat
     7561 tgtattgaga tcctgccagg ctgtatgccc aaagtgctgg gctgggtgac agagaaaatc
     7621 cgccaaccgc tgattgccgg tgggctggtg tgcgatgaag aagatgcgcg taatgcgatt
     7681 aacgcgggtg tcgtggcgct ttccaccacg aataccgggg tctggacgtt agcgaaaaaa
     7741 ttactttgac gggataatcg taaccaattg aatttggttt gatttgtagg ccgcacgcca
     7801 catccgacat tcagcgcctg atgcgacgct tgacgcgtct tatcaggcct acaagtcccc
     7861 aggccagaac cgtaggcagg atcatccggc aacacgttat tggcaatctg cgtgaatgtt
     7921 tgtcaccagt gcctccagca ccactttcca gtcatcaacc acgccaacat ctgcctgtga
     7981 aaataccgca gcgctggcgt catgattaat cgccaccaca aacttgctat tgcgtacgcc
     8041 cgccattaac gctgctgcgc cagaagcgcc aacaacaata cacacctcag gtgccagcag
     8101 atgtccggaa ataccaatca ctttttcagc atcaacgcca ccgttcatca ctcgcgcccg
     8161 actgtagccc acttcggctc ccagcttttc tgccagcata gcgatttcct ggttatctgc
     8221 ctctccgccc tgccccacca ccagcacccg tctggcctca gctaatgggt cgcgagtaac
     8281 atttttcaga tcctctgtgc taaccagcca gtccggaagc gcacccggga caatgttcag
     8341 ttgttgcatt ccagagggca acgtggcgtt tttcgctgct cccgcctgac gcgccaggga
     8401 aaggcatagc gggcgttttt ctgtttgcag cgtcgccgtt agcgcatttc cccagtggga
     8461 tttgcgcacg cttacggttg gaatatctaa cgaagtcacc tggcagatgc ttgcaccatg
     8521 taaacgccag gccagccggg tcgacagttc atcgccaaac gttcccggcg gaaagagaac
     8581 aacgtccgca ggcgttcgct gccactgttc caccaacgca tccagcacct gttccgccac
     8641 tacaggctga ggttcaatct gccagtgcgc cagggtacaa ccgctgaaat cctgcgccgc
     8701 cagccagctt gctattgcgg cattctcctg atttattgtc acaattgcga tattcatggc
     8761 tgcatccttt ggcgtaaata gtcctgccat aacttttgag ctttttctgc taccgtttgc
     8821 ccatcaatca gcgtcgctcc gcgtcgttgc tctgctcttg ccagttgcag acactgcatc
     8881 gctggcattt cggcagcaac agttttacgg ataatttctg ctttccccgc agccattcgc
     8941 tggcgcattc ccggtactgg caaggccact tcaccgcact gacgcacggc aatcaccgca
     9001 ggcaagcgaa cccggcaaca acgcaaccca tgttcagtac gctgttcgag ggtgataaac
     9061 agcgcgtcga gagtgaaacg ctccacctgg gtgaagcagg gccagcccag catttccgcc
     9121 agcaaaaatg gcgtttgccc attctgccct tcgctgcttt ggcagccggt aattatcaga
     9181 tccagcggat tctgatgctg ccattcggca atatggcgag cgacaaattc cggcgcaaag
     9241 cgcagatctg ccgccgtctc cagcaataca gcttcctcaa acccaagagc catgagatag
     9301 cgcagccagt gcaatgcccg ttcatccccc atgctcaacg cggttaaaga catcggcgta
     9361 ccgttttttc tctgcgccaa cagcagcgcg gcagcggcct gttcatcagc accgagtaaa
     9421 cttcgcagta gcgaaatatc cggtccgctt ttaccctgag ccgccgcctg ccactctttt
     9481 tccgccagca ttccggcatc cggttcggct ttaaacgcta acagaatgtt cattcgcacc
     9541 tcctacattt cccgccgcca ccagcgggag ggctttagtt tctggtgccc atagccaggt
     9601 cacaacaaaa ccaaccaaca acactgtcgc caggagcaat aaggtgactt gcattcccca
     9661 ctgcgccagc acccacggca gcaggccagt acttaccgcc gcgccaagtc gactcatggc
     9721 ggtggcaaaa ccgacgccca gcgagcgaat gtcagtagga aaactttccg caggcaaaat
     9781 gcccaccaga ttactgactg ccgaaatggt ggtgctgaag agaacaaaaa gcagcagcgt
     9841 taatgaactg ccggaaggca aacaggccat tactaccaga gttgccgcca gcagcaaaaa
     9901 acttcccagc aaaaatttgc gatgtgccag caggtgcgtc agaactaatc ccagcagcgc
     9961 gcccacaatt aacaacgcat taagcatcag gctggcagtc agcgcatctt ccagaccaat
    10021 agtctgggcg atagttggca gccaggtata aatcacaaac catgggatta cgaggcagac
    10081 aaagaagacg ctgttaaacg ccgtgcgccg ccagtaacgc gaagagaaca aggttttgat
    10141 gtgtttatgg gtcgccgtta ccacttcatc gcccagtaaa acatggggac caaaatagcg
    10201 atgcacgata gcgtgagctt ctgcaaaacg cccctggcgc agtagccagc gtggtgattc
    10261 tggcgttccc cagcgtaata acgtaatcaa caacgcgggc agagctgccg atgccagtag
    10321 ccagcgccag gcctccgggt tttcggaaat aaagtgatgt ccggcaatac ttgccagcac
    10381 atagcctacg gtccacacca cgctgaatgc gcccagcaaa ataccgcgat ggcggcgcgg
    10441 ggaaaattca gccagcaagg tgtgacctac tgaataatcg cctcccagac caatgccaat
    10501 caaaatgcgc agtccaataa gatgctctgg cgtggtggca aaaaattgta aaaacgaagc
    10561 aagcgtaatc agcaaaaagc tgaaggtgaa gattttttgc cgaccaatat ggtcggagat
    10621 ccacccaaga accaggctgc caaggaacaa accaaggagc gccgagccgc cgatcattcc
    10681 cgccataaac ggcgtcagtt gcatggcggg cgtaagctga ataatggcgt aaccaatgac
    10741 gccgagaaca taaccgtcgg tcaggtgtgc gccgaaagtg agcgcagcaa tgcggcagtg
    10801 aaaacggtta agcggtaaat catccattcg caccggtgaa gtgttcatct ttaccccttc
    10861 attgtccggt aataagaacg gcttctcgcc tgagaagccg aaaaggcaac gccacgtcat
    10921 cgcggaacgc tgcctgacac attatttttc aatcggatag atagtacccg tattcataat
    10981 gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc
    11041 cagtttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca tcgaaccgcc
    11101 gaggcgaatg gtttcttcac agatgatctt gttgagcgga ttgtggtact tgtcgatttc
    11161 ctcttccggc ttacagtcaa cgacgttgta atcgtagacg aagtacatgt tggtgccgtt
    11221 ctgatagcta tgagaggaat gaccgcccag catggtgatg tcgtcggcgt gcgggaactc
    11281 agtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc agcagccgga
    11341 cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt cggcagccac
    11401 tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagtttgc tgtccacgcg
    11461 ctggcactgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg ccttcgcaat
    11521 gcgaggattg ccttcagcca taaagatcag cacacattta ccgtcggcaa aatgggtgaa
    11581 gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat acccttccac
    11641 catgatttca cgcaggatgt tgaagccggt tttcatgtct tccaggatat agccgtagaa
    11701 gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga tatagcacaa
    11761 tgcaccttcg ttgccgatga tgatgtgacg aatgtccggg ccagccgcgc ggcgtggcac
    11821 gtttttaatg cgtgtgacgg tgccatcagc caatactgct tccagaccaa cgaccatatc
    11881 ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta ccaggccgcc
    11941 catctgagcc agcggctttg actgcggaga atgccccgtg gtgtaacctt tttcacgcaa
    12001 cgcattttcc agcacctcca gcggaacgcc acattgcgcc gtcgcctgca tattctcaat
    12061 atcaatatta atgatttgat tcatggcgga gccgtcgagc accaccgagt tttccacaac
    12121 ggtttccagc ccaccttcag tggcggaagc gccggtacgc ggcacaccgt taattttgtg
    12181 cgcattcata aaattcagca cacgggagac ttgttctgtg gaaccgagtt ttacgaccgc
    12241 tgccggaatc ggcaaagtat aaataccatg aatatccgga aatttacgaa aacggtcaat
    12301 actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa caatttcctt
    12361 tagctggtcg acaatcgctg cgcgagataa agacatagta attccttcct gataataaaa
    12421 atggaataat cattaataag agaaaacaga aaccaccgtt atggattagc gcactaaata
    12481 accaccatcg accactaata aatgcccatt gacataattt gaagccggac ttgcgaggaa
    12541 tacagctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa tatgatcaag
    12601 aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatatctg ttgcataata
    12661 gccaggggcg ataccattta cctgaatatt atattgacct agctcatcac aataagcttt
    12721 ggtgaacccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt gtccacctaa
    12781 gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga tcataatttt
    12841 tgcagcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca tcggatccca
    12901 gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat tgtttaccag
    12961 aatatcaact gtaccgaaac gctcacagca agcagcgata atcttctgcg gcgcgccttc
    13021 tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgtttttcaa tcatttcctt
    13081 tgtttcgccg ttatctttga cgaaactagg aataaagata tttgcgccag ctttggccaa
    13141 cgccatggca aatgcctggc ctaaaccgct attcccaccg gtaacaattg cggttttacc
    13201 tttcagtgag aaaaaatcca ttgagaacgc attgagagat tcgattgaca tagttagctc
    13261 caatttccgt aaggcaaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt
    13321 tctcattatc tctggtaact ggagataatt tttagcataa cggcataact gctaatgtga
    13381 tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta
    13441 ctctcacaca acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag
    13501 aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg
    13561 gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt
    13621 agcggcggcg ttagtttcga cctggcttat ttacgttata tttatcaaat tcccatggcg
    13681 aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg
    13741 gctattattc tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg
    13801 attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg
    13861 ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg
    13921 tcggtgtcga ttaaagccgc ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg
    13981 ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg
    14041 gtcttttctc gctttgcacc ggatgacagc gccagcctga aaacagtcat tatcatctac
    14101 agtgtggttt acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac
    14161 ctgcgcagtg ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt
    14221 ttgcgtatca gcaccacctg gtattgcagc atggtgattt ttggtgtctt cacaatctac
    14281 gccattctga gttactccac caactatctg accgaaatgt acggcatgtc gctggtggca
    14341 gcgagctaca tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttggc
    14401 ggcattatca ccacctacag taaagtgaaa tcccctaccc gcgtgatcca aatcctttcc
    14461 gtactcggcc tgctggcgtt aactgccctg ctcgtcacga actctaaccc gcaatcggtc
    14521 gcgatgggga ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg
    14581 tactgggcct gccctggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt
    14641 atttgttcgg tgattggatt cctgccggat gtcttcgttt acccgattat cggccactgg
    14701 caagacaccc tgcccgcagc agaagcctac cgcaatatgt ggctgatggg aatggcggcg
    14761 cttgccatgg tgattgtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc
    14821 gcccccgcaa tggctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga
    14881 aaaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc
    14941 tggaaggtaa cgtggtttgc gaaggcaaag gcttattaca gccgatgcac acgccagatg
    15001 ccgatactgc agaacatcct gacgacgatt tatgggcatc attatgtttt gccggtcacg
    15061 atttgatgag tcagtttgcc gggaataaag aagatattgt cggtattggt ctgggatcca
    15121 tccgttgctg tcgtgcgtta ttgaaagccg atggcacgcc tgctgcgccg ttgattagct
    15181 ggcaggatgc acgcgttaca cgcccttacg aacacactaa tcctgacgtg gcatatgtca
    15241 cctctttttc gggttatctg acgcatcgct taaccggcga gtttaaagac aatatcgcca
    15301 actattttgg tcagtggccg gtggattata agagctggac atggagcgaa gatgctgcgg
    15361 taatggataa gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg cctggcaccg
    15421 tcctcggaca tatcacacca caagccgcac tggcgacaca tttcccggca ggactgccgg
    15481 ttgtttgtac caccagtgat aaaccggtag aagctctggg ggccggatta ctggatgatg
    15541 aaactgcggt aatttctctt ggcacttaca tcgcattgat gatgaacggc aaagcactgc
    15601 cgaaagatcc ggtggcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg
    15661 aaggttacgg tattcgcaaa ggtatgtgga cggtgagctg gctgcgtgac atgttaggcg
    15721 agtcgttaat tcaggatgcc agggcgcagg atctttcacc ggaagattta ctcaacaaga
    15781 aagcctctag tgtgccacct ggctgtaatg ggctgatgac ggtgctggac tggctgacca
    15841 atccgtggga accgtacaaa cgcgggatta tgatcggctt tgattccagc atggattacg
    15901 catggatata tcgttcgata ttggaaagcg tggcgctaac gctgaagaac aattacgaca
    15961 atatgtgtaa tgaaatgaat cactttgcga agcatgtgat cattactggc ggcggttcga
    16021 acagcgatct gtttatgcaa atttttgccg acgtgttcaa ccttccggca cgccgtaacg
    16081 ccattaacgg ttgtgcaagt ctgggagcag cgattaatac agcggtaggt ctggggctat
    16141 acccggatta cgcaacggct gttgataaga tggttcgcgt gaaagatatc tttataccga
    16201 ttgagagcaa tgccaaacgc tacgacgcga tgaataaagg cattttcaaa gacctaacca
    16261 aacatactga tgtgatcctg aaaaaatcgt atgaagtgat gcatggggaa ttggggaatg
    16321 tggattcgat ccagagctgg tcgaatgcgt aagtgaggaa ggccgggcgg gaaactgccc
    16381 ggcctgaaca tacctgaatg gttatccccg ctgacgcggg gaacataagt tcgacaactt
    16441 aaaaaatgat aacaagtccg gtttatcccc gctgatgcag ggaacataat aaaaacttta
    16501 actagttcgg tttatccctg tttttacaag gaatattaac tacactcgat gtctttagaa
    16561 taaaagacaa tacgaatcgc actttatcaa ttttcactta aagta
//
LOCUS       DAZGXZ010000111        16563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000111 DAZGXZ010000000
VERSION     DAZGXZ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.111"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..272
                     /locus_tag="ACHKJF_003835"
     CDS             <1..272
                     /locus_tag="ACHKJF_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage repressor protein"
                     /protein_id="HFP0951106.1"
                     /translation="FDKGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGE
                     ITIRDITRLPGGRIFVEGGNRAFECKIEDIEIIGKIISLTVKYVK"
     gene            288..479
                     /locus_tag="ACHKJF_003836"
     CDS             288..479
                     /locus_tag="ACHKJF_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951107.1"
                     /translation="MLGKVFFVVLSCSLLLNPLTTYARNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
     gene            668..1720
                     /locus_tag="ACHKJF_003837"
     CDS             668..1720
                     /locus_tag="ACHKJF_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0951108.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETESKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAVNLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
     gene            complement(1825..2361)
                     /gene="rcdA"
                     /locus_tag="ACHKJF_003838"
     CDS             complement(1825..2361)
                     /gene="rcdA"
                     /locus_tag="ACHKJF_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP0951109.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            2445..3653
                     /locus_tag="ACHKJF_003839"
     CDS             2445..3653
                     /locus_tag="ACHKJF_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951110.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3653..4468
                     /gene="ybjI"
                     /locus_tag="ACHKJF_003840"
     CDS             3653..4468
                     /gene="ybjI"
                     /locus_tag="ACHKJF_003840"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP0951111.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            4554..4838
                     /gene="ybjH"
                     /locus_tag="ACHKJF_003841"
     CDS             4554..4838
                     /gene="ybjH"
                     /locus_tag="ACHKJF_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP0951112.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4879..6111)
                     /gene="mdfA"
                     /locus_tag="ACHKJF_003842"
     CDS             complement(4879..6111)
                     /gene="mdfA"
                     /locus_tag="ACHKJF_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP0951113.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            6396..6992
                     /gene="ybjG"
                     /locus_tag="ACHKJF_003843"
     CDS             6396..6992
                     /gene="ybjG"
                     /locus_tag="ACHKJF_003843"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0951114.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7050..7808
                     /gene="deoR"
                     /locus_tag="ACHKJF_003844"
     CDS             7050..7808
                     /gene="deoR"
                     /locus_tag="ACHKJF_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP0951115.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(7855..9057)
                     /gene="dacC"
                     /locus_tag="ACHKJF_003845"
     CDS             complement(7855..9057)
                     /gene="dacC"
                     /locus_tag="ACHKJF_003845"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0951116.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            9304..9930
                     /gene="gstB"
                     /locus_tag="ACHKJF_003846"
     CDS             9304..9930
                     /gene="gstB"
                     /locus_tag="ACHKJF_003846"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP0951117.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(9927..11042)
                     /gene="yliI"
                     /locus_tag="ACHKJF_003847"
     CDS             complement(9927..11042)
                     /gene="yliI"
                     /locus_tag="ACHKJF_003847"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP0951118.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPRALAFLP
                     DNHGMLITLRGGELRHWQVGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRSGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(11153..11536)
                     /gene="bssR"
                     /locus_tag="ACHKJF_003848"
     CDS             complement(11153..11536)
                     /gene="bssR"
                     /locus_tag="ACHKJF_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP0951119.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            11749..13074
                     /gene="rimO"
                     /locus_tag="ACHKJF_003849"
     CDS             11749..13074
                     /gene="rimO"
                     /locus_tag="ACHKJF_003849"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP0951120.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(13121..14449)
                     /locus_tag="ACHKJF_003850"
     CDS             complement(13121..14449)
                     /locus_tag="ACHKJF_003850"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0951121.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(14457..>16563)
                     /locus_tag="ACHKJF_003851"
     CDS             complement(14457..>16563)
                     /locus_tag="ACHKJF_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0951122.1"
                     /translation="QRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQ
                     VNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRING
                     KDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNS
                     EGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLL
                     VVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMK
                     DAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDE
                     RNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYR
                     NLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIA
                     RNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGF
                     INELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGL
                     KANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSI
                     KVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYL
                     YQKPMPFSAWDKSGKLVKE"
BASE COUNT         4306 a         4112 c         3860 g         4285 t
ORIGIN      
        1 tttttgataa gggatttcta ccctctacgt ttaaaaaacc ttttgtcatc acagataaca
       61 attctgaatt tatttgtgat aaagaatttg atgatatacg tgatggtaaa tgggtaataa
      121 gtattgatgg cgaaataacg atccgtgaca ttactcgttt acccggtgga agaatcttcg
      181 tcgagggtgg aaacagagcc ttcgaatgca agatagaaga cattgaaata attggtaaaa
      241 ttataagttt aacagtcaag tatgttaaat agtaccggga ggaaactatg cttggtaagg
      301 tattttttgt ggttttgtca tgttctttgt tattaaaccc actaactacc tatgctagaa
      361 attatccctg ctcagggaaa aagggaggtg tttctcactg tacctcagat ggcaaattcg
      421 tttgcaatga tggaactatt agtaaatcca aaaaaatctg tactaaaaac tcacgataac
      481 ttttgctttt atatctgcgt ctaaaataaa aatgagccgc aggttaaccg caaaagttac
      541 atgctcacat agcaaaaaga atagccaact tcattatggc ttcagtgaga tgtatggtcg
      601 taggatttca tacattgaca ctggttatat atacagtaaa aatgctctct actggagggc
      661 attttttatg gcagtacgaa aactcaccac agggaaatgg ctttgcgaat gttaccccgc
      721 cggacgaagt gggcgtcgtg tgcgtaaaca attcgccacc aaaggcgaag ctctggcttt
      781 tgagcgtcac acgatggaag aaaccgaatc aaagccctgg ctgggcgaat cagtggatcg
      841 tcgaaccctg aaagacgtgg ttgagctatg gttcaaacta catggtaaat cactgacagc
      901 tgggcagcat gtctatgaca aattgctgct gatggttgac gctctgggca atccccttgc
      961 aactgatcta acctctaaaa tgtttgccca ctatcgagat aaacgcctga caggtgagat
     1021 ctacttcagc gagaaatgga agaaaggagc aagcccggtc accatcaacc tggagcaaag
     1081 ctatctaagt agtgttttta gcgaactatc ccgcctgggc gaatggtcgt atccgaaccc
     1141 actggagaac atgcgaaaat tcaccatcgc agaaaaagag atggcatggc ttacccatga
     1201 gcagattgtt gaattactgg ctgattgcaa acgtcaggac ccaattctgg cactggtagt
     1261 taagatatgc ttaagcacag gcgcacgctg gcgagaagcc gtaaatctta ctcgttcaca
     1321 ggtgaccaaa taccgaatta cctttgtaag aacgaagggg aagaaaaaca gaagcatccc
     1381 tatcagtaaa gagctttacg aagagatcat ggcgcttgat gggttcaatt tcttcacaga
     1441 ctgctatttt caatttttat ccgtgatgga aaaaacgtct atcgtgctcc ctcgcggtca
     1501 actcacacac gttctgcgcc atacgtttgc ggcgcacttc atgatgtcgg gtggaaacat
     1561 tctggcctta caaaaaattc tcggacacca cgatataaaa atgactatgc gttacgcaca
     1621 tctggcaccg gatcatctgg aaacggcgct ccgtttcaat cctctggcaa cgctgccaag
     1681 tggcgacaaa gtggcggcag cggttggcat taccccgtaa taaccaccac tgaccaccac
     1741 cccaacttat tgtttttact ctaacttatt gttttcatta acccgtttac ataaatgggt
     1801 tttttgttgt ctaaaactta tctactaccc tgcaaccctc tcaaccatcc tcaaaatctc
     1861 ctcgcgcgat agcggcttac ggtcggtgac aaaatgcagc gtcatcccct caataaacgc
     1921 atcaagcgcg cgggcggttc cgggttcaaa ccattgttcg agcgtttgct gactgcgctg
     1981 catccagttt tgcattaccg tttttaatag cggttttcgg ctagccagcg cgtagagctg
     2041 gtacatcagc tccatattat ccggcgttgc aacctgtgag ctgtagatca tatcggtgat
     2101 agcctggcat gcgccttgag catcactaac atcgctaaaa aatgcctgat attgccggga
     2161 cataatctca gtaaaactgc tgaacgcctc cagcaacaac tcatcaattc ctgaaaaata
     2221 gtaggtcatc gatcccaacg gtaccccggc aagggtagca attttgcggt gcgtaacagc
     2281 atgtattccg taaagtttca ccgcctccag cgtggcctgg ataatttttt ctcgccgttg
     2341 cggatcgtta gcgcgacgca tgtcatttcc tctctctgca attgtgtaca aatgtacaca
     2401 accttgctaa tgttgtcttc cctcttctta ttttgacgtg atctatgacc gtaaattctt
     2461 cacgtaatgc attgaaacgc cgaacctggg cgctgtttat gttcttcttt ttgccaggcc
     2521 tgttaatggc gtcctgggca acccgtacgc ctgctatccg cgatattctc tctgtctcga
     2581 tcgctgaaat gggcggtgtt ctctttggtc tgtcgatcgg ttcgatgagc ggtattctct
     2641 gctcggcgtg gttagtgaaa cgctttggca cgcgtaatgt catcctggtc acgatgtcct
     2701 gcgcattgat cgggatgatg atactaagtc tggcactctg gctgacatcg cccctgctct
     2761 tcgcggttgg tctcggcgtc tttggggcaa gttttggttc tgcggaagtg gcgataaacg
     2821 ttgaaggtgc tgccgttgag cgggaaatga ataagacggt tttgccgatg atgcacggtt
     2881 tttatagcct gggcacgctg gcaggcgctg gtgtcgggat ggcactgacg gcctttggcg
     2941 ttccggcaac ggtgcacatt ttattggcgg cgctggtagg catcgcgcct atttatatcg
     3001 ccattcaggc aatccctgac ggtacgggca aaaatgctgc cgatggcacc cagcatggcg
     3061 aaaaaggcgt acctttttat cgcgatatcc agttgctgct gattggtgtt gtggtgctgg
     3121 cgatggcctt tgccgaaggt tctgccaacg actggttacc cttattaatg gttgatggtc
     3181 acggttttag ccccacttcc ggctcactga tttatgccgg ttttaccctg gggatgaccg
     3241 ttggacgctt caccggcggt tggttcatcg accgttacag tcgcgttgcc gtggttcggg
     3301 ccagtgcact aatgggggcg ttgggtattg ggctgattat ttttgtcgat agcgcctggg
     3361 tcgcaggagt gtctgttgta ctttggggac tgggtgcctc gctgggcttc ccgctgacca
     3421 tttctgccgc cagcgatacc ggccccgatg caccaacccg cgtcagtgtg gtagcaacga
     3481 ccggttatct ggctttcctc gtcgggccgc cgctgctggg ctatctcggc gaacattatg
     3541 gattacgtag tgcaatgctg gttgtactgg cgctggttat tctcgcggct attgtcgcga
     3601 aagccgtcgc caaacccgat accaaaacgc agacggcgat ggagaatagt tgatgagcat
     3661 taaattaatt gcggtagaca tggatggtac tttcttaagc gatcaaaaaa cctataaccg
     3721 tgagcggttt atggctcagt atcagcaaat gaaagcacaa ggcattcgct ttgtggtcgc
     3781 cagcgggaat caatattatc agttgatctc tttcttccct gaaattgcta atgaaattgc
     3841 ctttgtggct gaaaacggcg gctgggtagt gagcgaaggc aaagatgttt ttaatggcga
     3901 gctgtcgaag gatgcgttta ctactgtcgt ggaacatttg ctgacgcgcc cggaagtgga
     3961 aattattgcc tgcggaaaaa atagcgccta tacgctcaaa aaatatgacg atgccatgaa
     4021 aacggtggcg gaaatgtatt atcaccgtct ggaatacgtc gataactttg acaacttaga
     4081 ggatatcttc tttaagtttg gcctgaatct ttccgatgaa ctgattccac aagtacaaaa
     4141 agcattacat gaggccatcg gcgatattat ggtgccggtc cacaccggca acggcagcat
     4201 cgatctgatt atccccggcg tacataaagc caatggcctt cgccaactgc agaaattatg
     4261 gggaatagac gacagcgaag tggtggtctt tggcgatggc ggtaacgata ttgagatgct
     4321 gcgtcaggca ggctttagtt ttgcaatgga aaatgccggc agcgcggtcg tcgcagcggc
     4381 aaaataccgg gcaggctcca ataaccgtga aggcgtactg gatgtgatcg ataaagtgct
     4441 taaacacgaa gcgccatttg accaataaaa acggcagcac gcccggcaac acaatacctg
     4501 aatgaaggct gcataattcg cggaaattaa cccgctgcat tatttcaggc gaaatgatta
     4561 tgaaaaattg tctactgttg ggcgcgcttt taatgggctt tactggtgtg gcgatggcgc
     4621 aaagtgtcac cgtcgatgtg ccgagtggat acaaagtggt tgtagttcct gattcagtca
     4681 gcgttccaca agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg gctcctgcgc
     4741 cagctccggc ttaccgcccg catccttatg ctcgccatct tgccagcgta ggtgaaggga
     4801 tggttatcga acaccagatt gacgaccatc accattaatt cgagaatgcc tgatcgcaca
     4861 aagcagtcag gcattttttt acccttcgtg agaatttccc atctgtttat cttttaaaaa
     4921 gataaccatc agcgacagcc acaaaattcc gttgacaagg ttgaagagat taaactgtcc
     4981 gttgcccccg ttcagccagg catgtttgct gatttcaata ccaacggtaa agatcagcat
     5041 ttgcagcatt cccatcgccg cagaaaccgt acctttactc atatcgctgg caaacagggt
     5101 taatcgcacc agtcccgcat tcgccagacc aataccgaaa gcataaatac ttaacccggc
     5161 agtcatccat aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga ccaacagacc
     5221 aatcataatc ggccagccgc ccataattat cagcgaacgt acggtgcggc gcgaggtcaa
     5281 acgcgctaac aacaagttac ccgcaattaa cgccccgaaa ataggcactt gcagcaagcc
     5341 atattcatag ctgctcaact gctcgccggt aatgatgata atcggcgact gggcgatcca
     5401 cgccagcaat ggcaggctaa cgaatcccag cgccagcgcc cccgccacaa agcggccgtt
     5461 cttcagcacc agcttatagt cacgaccgag ttctttcagt gacagtttct cgcctatacg
     5521 cgtggcggtt tcaggcatgg cttgttgcag accgaaaaag gagatcgctg ccaatgcggc
     5581 aaacaagaca aacatccctt cccagggcag cacatggatc cacgccgcgc ccaccagcgg
     5641 accaagtagc ggagcaatca gcgccacgtt cgccatcagc gcggtgatct tgatacaaac
     5701 cgcctcttcg aaggattcct gaattgcggc gtatcccaca gcgccaatga aacagaggct
     5761 tatgccctgc aagaagcgca acagagtgaa ttgttcaatg ttttgcgcca gtaatattgc
     5821 cagacaggtg atgataaacc acaccacccc cgccagcatc accggacggc gaccaatacg
     5881 atccgacagc ggccccagga gccattgtaa aaacatcccg cccgccagat atgcggtcat
     5941 tgaagtagga acccaatcaa tgcccgcctg atattgttcc accacggcca acataccggg
     6001 ttgaatcata tcgttgccga tataggttga aaattcgtaa agcaccagac agagagggaa
     6061 aagtaacgcc tgacgtccaa gcctagcacc ggaagctaat ttattttgca tgcaatttct
     6121 tcgccaataa taatcgcgca gagtttaata aaagcacagc taacgagaaa gcgaattttg
     6181 tagctgaaac cacggttaag cacattctta cattattgcg agtatagcta cgctttcttt
     6241 aagttttatt taacctatgc ccgttacaat tacccaccgt aaacaggccg cttgagggaa
     6301 ataagacgat gccgctttac ccagtttaac ctgcacttta ttctcaacga cttgcctgta
     6361 ttggctccct tttaatcact ttgcgtcggg aagttatgct ggaaaatttg aatctctctc
     6421 tattctctct tattaacgcg acgccagact cggctccgtg gatgatctcg ttggcgattt
     6481 ttattgctaa agatttgatt accgtggtac cgctgctggc cgcggtgctt tggttgtggg
     6541 ggcttacagc acaacggcaa ctggtgataa aaatcgctat cgcgctggcg gtcagcctgt
     6601 ttgtgtcctg gacgatggga catctttttc cgcacgaccg accctttgtc gaaaatatcg
     6661 gctataactt cctgcatcat gcggcggatg actcattccc aagcgaccac ggtacggtga
     6721 ttttcacctt tgcactggca tttttatgct ggcatcgcct gtggtccggc tcacttttaa
     6781 tggtgctggc cgtcgtcatt gcctggtcgc gcgtttatct tggcgtgcac tggccgctgg
     6841 atatgctcgg tggattgctg gcaggtatga ttggctgcct tagcgctcag attatctggc
     6901 aagcgatggg gcataaactc tatcaacgtc tgcaatcgtg gtatcgcttc tgttttgcat
     6961 taccgatccg caaaggctgg gtgcgtgact gatctctgct aaaaagtgta gtattgagcg
     7021 gctcgcttca ataactattc agagggatta tggaaacacg tcgcgaagag cgtatcgggc
     7081 agctgctgca agaattaaaa cgcagcgata agttacatct taaagacgcc gccgccctgc
     7141 ttggggtttc ggagatgacg attcgtcgcg atctgaacaa ccacagtgcg cccgtcgttt
     7201 tgctcggcgg ctatattgtt ctggaaccgc gcagtgccag ccattacctg ttaagcgatc
     7261 aaaaatcccg cctggtggaa gaaaaacgcc gggcggcaaa aatggctgcg acactggtag
     7321 aacccgatca gactctcttt tttgactgtg gcaccaccac gccgtggatt attgaagcga
     7381 ttgataatga aatccctttt accgccgttt gttattcgct aaataccttt ctggcgctga
     7441 aagagaaacc ccattgccgc gcatttctgt gcggtggtga atttcacgcc agcaacgcca
     7501 ttttcaaacc catcgatttt cagcaaacgt tgaataattt ttgcccggat atcgcttttt
     7561 attccgcggc gggcgtgcat gtcagtaaag gcgctacctg ttttaatctt gaagagttgc
     7621 cggtaaaaca ctgggccatg tcgatggcgc aaaagcatgt gctggttgtc gaccacagta
     7681 aatttggcaa ggtgcgtccg gcgcgcatgg gtgacctgaa acgctttgat attgtggtga
     7741 gtgattgttg cccggaagat gagtatgtga agtacgcgca gacgcaccgt attaagttga
     7801 tgtattaatg acgtataacc ggatgacgtt tcgcgccatc cggttatcag aagattaaga
     7861 gaaccagctg ccgaaccact gatggaattt catcatcacg aaatcccaca tccgaccaaa
     7921 gaatccgccc tcttccacat tttccatcac gatcagcgga cgctgctcaa tggatttacc
     7981 gttaagctgg aaatcaatgg tcccgacaac ctgacctttt ttcagcggtg cggtaagctg
     8041 cggttccgtt aacgtgtagc tcgccttcag gtttttcagc tgcccacgcg gtatggtcac
     8101 tgagcccgct tcacctgccc caagattcac ttcgctctta tcaccaaacc agacgcgctg
     8161 agtcacaaag gtggcatcag gtttaattgg cgtcacggtt tcaaagaagc ggaaacccca
     8221 ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgccc ccagcactac
     8281 ggagattaaa cgcatatcgc cctgggtagc cgaagcaacc agattatatc ccgcgcctgc
     8341 cgtggttcct gtcttcatgc catcaacatt cagattgctg ctccacagca gacggttacg
     8401 gttaggctga cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc
     8461 cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg
     8521 ccccggcgca tccagaccgt gcaccgtctg gaaggtagtg ttggtcagac ccagtttttt
     8581 ggcataacca ttcatcaaac caataaatga ctcctggctc ccggcaacgt aatcggccag
     8641 cgcaatacag gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga
     8701 aacctgatcg cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt
     8761 cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag
     8821 cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcgggat ccagtttctc
     8881 atccgcgttg ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg
     8941 cgcatccacg ctcggcgctt caacggtttg ttccgccgcg aatgccgttg gggcaaaaag
     9001 gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa
     9061 tgccacccga gtatccattc aaaaaaaata cgttatatcg ccgtatgata agttcggtga
     9121 ttaataacgc acacacaaac ttaaagaaac cgtaagttgg taaagttttt aaagtttatg
     9181 caatatcctg ccgcttcgct gcttctggtg tcattttttt gccagctgtt gcttaaaagt
     9241 taactttact tcaccatcgg agtcctgttc agcctgtggc tgcctgtcgg aaaaggggtg
     9301 agtatgatta cgctgtgggg tcggaataat tcaactaacg taaaaaaagt attgctgacg
     9361 ctcgaagaac tggaactacc ttatgagcaa attctcgcgg gccgtgagtt tgggataaat
     9421 cacgatgctg attttctggc gatgaaccct aacgggctgg tgccgttgtt gcgtgacgac
     9481 gaaagtgatc tcattctttg ggaatcaaac gccattgtcc gctatctggc ggcacagtac
     9541 gggcaaaaac gcctgtggat cgactcaccg gcacgtcgtg cggaagcgga aaaatggatg
     9601 gactgggcaa accagacact cagtaatgct catcgcggga tcctgatggg attagtcaga
     9661 acaccacccg aagagcgcga tcaggccgcc attgatgcca gttgcaaaga gtgcgacgcc
     9721 ctgtttgccc tgctcgatgc ggaactggca aaagtaaaat ggttctctgg cgacgagttt
     9781 ggtgtgggcg atatcgctat cgcaccgttc atctacaatt tgtttaacgt tggcctgacc
     9841 tggacaccgc gtccaaatct gcaacgctgg tatcagcaac tcactgaacg ccccgcagtg
     9901 cgcaaagtgg tgatgattcc cgttagctaa ttgcgtgggc taactttaag taattcccca
     9961 ctggactcgt cggtgagaac gtataaataa ccgtcgggtc cagagcgaac atcacgaatt
    10021 cgctgccctc tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc gttgacgctc
    10081 atcacaatga catctttatc tttcagcgcg cctataaata atttttgctg ccactggggg
    10141 aatttatcgc tgttatagaa ggccatgccg ctcacagcgg gcgaatcttt ccagtaaaaa
    10201 acaggttgct cggtcccggc gacgatctcc cctttcgctt ccggtatctt aaagcctgaa
    10261 tagttgattc cccaggttgc cagcggccag ccgtagtttt tgcctttttg cgggatatta
    10321 atttcatcac caccgcgcgg gccatgttca ttcagccaca gtgcattact ccacggattc
    10381 atcgccattc cttgcggatt acgaatgcca taagaccaga tctcggcgcg cgcaccggat
    10441 tcctttataa aaggattatc atccgggatt tcgccctggt cggtcagacg caccagtttg
    10501 ccctgtaatt tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc cagagcaata
    10561 aaaagataac ctttaccgtc gaataccagc cgcccgccaa aatggttgcc ggtagacagt
    10621 tttggcatct ggcggaagac ggtgcggaag tcggtcactt ttgagagatc atcacttaag
    10681 cggccataac ccacagcagt tccggctttg ccatcatcgc caacttcgga ataacttaac
    10741 cagatgcggc gagactgagc aaaatcaggc gctaaaacca cgtccagcag gccgccctgc
    10801 ccgtgcgccc aaacgtccgg aactccggaa agcggcgcag ataatccttt tcctacttgc
    10861 cagtgacgca actcgccgcc gcgcagagtg attaacatac cgtgattatc gggtaaaaag
    10921 gccagtgccc tgggatggtc gagtttgtct tgcagtactt cgacatttac cgttgcagga
    10981 gccgcccaga gagcggaaga aagacaaata aggggcacaa ggaaaaagga ttgtcgatgc
    11041 ataacgcctc cggattgact ctggcttaaa gcgtagtcag tggaggagat aacaaattca
    11101 tttttacaaa aacttaaaca tgaaggggga gacgctttct cccccttagt tttcaggcct
    11161 tctcaagcat ggcgtgcttc tgcaggctct ggatactcag cgttaagctc atcagacaat
    11221 tttcaagctt atcggcgttg acggtaataa cagtcgggca atcatggtgc ccactcatca
    11281 aacatactgc ggctgtcgct aatgcttctt cagcatgatg aagagcactc cactcttcct
    11341 gatccagatg aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc agtttaaaaa
    11401 agttatctcg tagatgattg ctttcgctga cggacatgta tccttttgcc ttcctcagtt
    11461 gcacgtatgc ggcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga tcgattcgct
    11521 gtctgtcaac gaacatactt cgatcctcct ctttccagcc ttgttgcaat taaatagtat
    11581 ggctattttt tgagcaaaat gctgtccagg atcaattctt cacgccaaat taacaaaatt
    11641 tacatttcct tcgcctccgc cagcgaatga gattgcgata caaattttat ccagaactct
    11701 gtagaatccc tgccctgacc tttcctgtaa ctgaatattt tttaagctat gagcaaagta
    11761 actccccagc cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct tgtcgattca
    11821 gagcgtattc tcaccgaact acgcactgaa ggttatgacg tggtaccgag ctatgacgat
    11881 gcggacatgg tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca agaatcactg
    11941 gaagccattg gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtaaccgg ttgtctgggg
    12001 gcaaaagaag atcagatccg cgaagtccac ccgaaagtgc tggaaatcac cgggcctcat
    12061 agctatgagc aggttctgga gcacgttcat cactacgtgc caaaaccgaa acacaaccca
    12121 ttcctgagcc tggtgccaga acaaggtgtg aaactgacac cgcgtcatta tgcctatctg
    12181 aaaatttcag aaggctgtaa tcaccgctgc accttctgca ttattccgtc tatgcgcggc
    12241 gacctggtga gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct ggtagatgcg
    12301 ggcgttaaag agattctggt gatctcgcag gatacttccg cctatggcgt tgatgttaaa
    12361 catcgtactg gcttccacaa cggcgagccg gtaaaaacca gcatggtcag cctgtgcgaa
    12421 cagttatcga aactggggat ctggacacgt ctgcactacg tttaccctta tccgcatgtg
    12481 gacgacgtca tcccactgat ggcagaaggc aaaatcctgc cgtatctgga cattccgttg
    12541 cagcacgcca gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt agatcgccaa
    12601 ctggcgcgca tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg ctcaaccttt
    12661 attgtcggct tccctggcga gacagaagaa gatttccaga tgctactcga cttcctgaaa
    12721 gaagcgcgtc tggatcgcgt tggctgcttt aaatacagcc cggttgaagg tgcagacgcc
    12781 aatgccctgc ctgaccaggt tccggaagaa gtgaaagaag aacgctggaa ccgtttcatg
    12841 cagctgcagc agcagatttc cgccgagcgc ctgcaagaga aagtgggccg tgaaattctg
    12901 gtgattatcg acgaagtgga cgaagaaggc gcaattggtc gcagcatggc agatgcaccg
    12961 gaaatcgacg gcgcggttta tctcaatggt gaaaccaacg ttaagccggg tgatatcctg
    13021 cgtgtgaaag tcgagcacgc cgatgagtac gatttgtggg gtagccgggt ttaattaatt
    13081 caggccgggc aaaatcattt gtccggcctg cgaataatga ttactgccag ctaatctgtg
    13141 ccctgccgtt tttcttactc ttatacagcc gctcatccgc cagcattttc aagcgttcga
    13201 cggtaggaca ttcatttgaa tgtgctacac cgatactggt acttaaatta atgcttccca
    13261 cctggaaatg aacgatttct tttagaatcg atagcaactc ttccagcgtt ccacccgtat
    13321 aaatcaccgc aaattcgtca ccgccgaagc ggtagacttt acctttatct accgggatct
    13381 gtgatttaag gattttcacc actttcatca acacttcatc gcccagcaaa tgaccgaagg
    13441 tgtcgttaat gtttttgaat ttatcgacgt caaacaggca caaataaccg cctttttccg
    13501 gcagcgcttt taaatcttcg tcaaaggatt ttctgcccat tgctttggtc aggtcgtcca
    13561 ggaacgccga gttatacata ctgtagagtt ttttgacatc ggcagccgaa aagaaaagcc
    13621 cgccatccag tacgcgttca aaatggttat taatgatggc ttctttctcc acaataccac
    13681 gattaatcac tctgcgtacc agatagaggc aactggcacc cgatatcact gccaccaatg
    13741 cggcaaagaa aataatcaat gtacaaaccc gcaaataaac atctttttta tacgcaaata
    13801 catacaatcc ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg gtgaagatgg
    13861 cgtttttagc attatattca tcaccggtca ccggtttacc gttcttaaac agagagaaca
    13921 tcaggttctc gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc tccagaccaa
    13981 gcaaaaatat cagactacga ttgtgagtta ccggatagag gtaatagacg aaggttttac
    14041 cgtcaaaaga gataaaccct gtatttttga catgtcgata cttcacaacc tgcataatat
    14101 ctgcacaata atttttctcg aactccttta cctgaataca ttcgtcactg gaaatcagat
    14161 aaggaccgac atcgtacaag taagagggtg aaaccgagtc agcgtaagct gtgcgtaagc
    14221 caaccaacag ctgttcctcc gtgggattta gcgtattgat aagatcaatt gccacgctac
    14281 gccgatattc agcaatattt gccaccgcaa aagcagtatc aataacggac tgtttcaggc
    14341 tatacacccg ttcctttacc atttgagtgt agatcccgca ggcaactgct gaaatcatga
    14401 taaaaatcag cagactgact gtaagtacga acttattgat tctggacata cgtaaactac
    14461 tcttttacta attttccact tttatcccag gcggagaatg gcatgggttt ctggtacaga
    14521 tacccctgag cctgaacaca tcctaattta attaattcgc gcgcaacgtc ttcgctctct
    14581 acgccttccg caatcaccgg gatccggtta tagcgtgcca gatcgaaaat aatgcgcaca
    14641 atttttcccg cagtaccgaa aatatcgccg gtaaattgcg agtccacctt tatgctgtct
    14701 ggattaattt cataaaaacg tttcaggttt gacaaaccgg tgccaaagtc atcaatcgat
    14761 atccgtaccc cttgtttacg cagttgttca atattgagat aaaaatgttc attaattcgc
    14821 tcgatcacgg tttcagtgat ctcaacacag aggctgttcg ccttcaggcc attttctttc
    14881 atgatggtgg taaaacgctc atgaaagtgc ggttcgttta actgccaggg cgaaacatta
    14941 atatgcagta agaaatcatc cttcagcgac gcacgctgac taaaatgtct gaattcaccc
    15001 attgccgttt taataatctg ataacccagc tcattgataa acccgatatc ttcagcaatg
    15061 gtaatgaact tcagcggtga aatgatcccc cgctccgcag aaacccaacg gcacaacgcc
    15121 tccgcgccca gaatggcgcg ggtgttaata tcaacgatcg gttgatagac gaggaatagt
    15181 tcgccaattt ccattgcatg gcgaatatta cgcgcaatcc agatattctc ttctatttcg
    15241 ttgagtttcg cgtcatcaca aaaaataaac tcggagaaca tatggtcacg gatactggaa
    15301 atcgccagtt tcagtttttc tgcgtaactc caggcgtttt ccccctgcaa aggttcacaa
    15361 atggcagact tatggatgcg cagattgcgg tagccaaagc tgctgtcaaa atcgtttacc
    15421 atcgctattt tacgggtaaa tgtctgtaca ttttcccgac acagaagtaa atagagatcg
    15481 gcattgaatg tcacgatatc gcagcattgc gcatatttct cggcaattcg ctgacacaat
    15541 ttcctgacga ttttattgcg ttcatcaaca ccaaacaccg cttccacggc ttccagacca
    15601 cacagtttga tgatccccac agccagatac tgattattgt tggactcaat aatcgccata
    15661 tttttaataa aaccgctttc ctgatcgtaa gagatcagtg agagcaattt atttgaccgt
    15721 tcaatttcgg catctttcat ttccgcgatc gaatttttga tgctgataat ctctttcgca
    15781 tcgccttcgc gaattttcag agcctcgatc tgatccgttg agtcgggcaa aaacgccagc
    15841 gtattaacca gctcggtcat ttcagaaaat tgccgacgga acacacgagt ggtcatgaaa
    15901 tataccacca gcaacacgat aataatgaac gcaaacggta gataaaaaag tttatctgat
    15961 gcgttcaata aattaccgcc aggataataa aaatagattt cgaacaatgc attatttggc
    16021 actgctgatg tctttgcaat atactccgta tcgacagaat caataatatt ataattacct
    16081 tcgctattat aaataatcag tttttcacga gcgagttgat tcttggcaat gatctcatga
    16141 ccttgtttca aaaatagaac ttcatcatta gcgctgattt gatcaagata ctcgttcaga
    16201 taccgccagt ttaaatcgac atgaatttta tctaccgcta acaaatcgtg atctttgccg
    16261 ttgatacgta gcgtaaaaga gatacatttt ttacccggac gatcaaagca attgtatgcc
    16321 ggattccagc tgttgttaga gtcatctgaa gccaccgacc acggtcgttt cgatgggtta
    16381 aaatcggcaa aaataacccg ccctgactcg tcgacaagcg gtccatttac ttgttcctga
    16441 tgataaacaa cgattttatc attcgccctc cgcgaaaaga gataatattt attatcatca
    16501 agtaccagtc ccaccgagtc taaaaatggc atcatttcca ttttcagaat cacggcgcgt
    16561 tga
//
LOCUS       DAZGXZ010000112        16507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000112 DAZGXZ010000000
VERSION     DAZGXZ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.112"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(74..880)
                     /gene="trpA"
                     /locus_tag="ACHKJF_003852"
     CDS             complement(74..880)
                     /gene="trpA"
                     /locus_tag="ACHKJF_003852"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP0951123.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLVALKVFVQPMKAATRS"
     gene            complement(880..2073)
                     /gene="trpB"
                     /locus_tag="ACHKJF_003853"
     CDS             complement(880..2073)
                     /gene="trpB"
                     /locus_tag="ACHKJF_003853"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP0951124.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2085..3446)
                     /gene="trpCF"
                     /locus_tag="ACHKJF_003854"
     CDS             complement(2085..3446)
                     /gene="trpCF"
                     /locus_tag="ACHKJF_003854"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP0951125.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3447..5042)
                     /gene="trpD"
                     /locus_tag="ACHKJF_003855"
     CDS             complement(3447..5042)
                     /gene="trpD"
                     /locus_tag="ACHKJF_003855"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP0951126.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5042..6604)
                     /gene="trpE"
                     /locus_tag="ACHKJF_003856"
     CDS             complement(5042..6604)
                     /gene="trpE"
                     /locus_tag="ACHKJF_003856"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP0951127.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6626..6719)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6696..6740)
                     /gene="trpL"
                     /locus_tag="ACHKJF_003857"
     CDS             complement(6696..6740)
                     /gene="trpL"
                     /locus_tag="ACHKJF_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP0951128.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6878..7759
                     /gene="rnm"
                     /locus_tag="ACHKJF_003858"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6878..7759
                     /gene="rnm"
                     /locus_tag="ACHKJF_003858"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP0951129.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVK
                     GVWQLWEQPQNTTEREL"
     gene            7756..8376
                     /gene="yciO"
                     /locus_tag="ACHKJF_003859"
     CDS             7756..8376
                     /gene="yciO"
                     /locus_tag="ACHKJF_003859"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP0951130.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8477..9349
                     /gene="rluB"
                     /locus_tag="ACHKJF_003860"
     CDS             8477..9349
                     /gene="rluB"
                     /locus_tag="ACHKJF_003860"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP0951131.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9389..9979)
                     /gene="cobO"
                     /locus_tag="ACHKJF_003861"
     CDS             complement(9389..9979)
                     /gene="cobO"
                     /locus_tag="ACHKJF_003861"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP0951132.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(9976..10734)
                     /gene="yciK"
                     /locus_tag="ACHKJF_003862"
     CDS             complement(9976..10734)
                     /gene="yciK"
                     /locus_tag="ACHKJF_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP0951133.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10954..12003
                     /gene="sohB"
                     /locus_tag="ACHKJF_003863"
     CDS             10954..12003
                     /gene="sohB"
                     /locus_tag="ACHKJF_003863"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP0951134.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12039..12290)
                     /gene="yciN"
                     /locus_tag="ACHKJF_003864"
     CDS             complement(12039..12290)
                     /gene="yciN"
                     /locus_tag="ACHKJF_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP0951135.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12670..15267
                     /gene="topA"
                     /locus_tag="ACHKJF_003865"
     CDS             12670..15267
                     /gene="topA"
                     /locus_tag="ACHKJF_003865"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP0951136.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15477..16451
                     /gene="cysB"
                     /locus_tag="ACHKJF_003866"
     CDS             15477..16451
                     /gene="cysB"
                     /locus_tag="ACHKJF_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP0951137.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4024 a         4191 c         4383 g         3909 t
ORIGIN      
        1 aaatgaattt aggatttttc cggcttcatt aaagaaagtt aaaatgccgc cagcggaact
       61 ggcggctgtg ggattaactg cgcgtcgccg ctttcatcgg ttgtacaaaa actttcagtg
      121 ccaccagcat tttctctggc tcattaatat gttgctcgat gattttaaca atggccgaac
      181 cagaaatcgc gcccgcagct cctgcatcaa tcgctgcttt tacctgatcc ggggcggaaa
      241 taccaaatcc ctgcaatgga ggtgcagcgt tgtactcttt cagcttcgca accagatgat
      301 tgaggggtaa cgcggcgcgg ttttctgcgc cggtcacgcc tgctcgtgac agcaaatagg
      361 tgtaaccacg accgtaagag gctatctggc gcagcaggtc gtcatcggca tttggcgggc
      421 agatgaagat aggtgcgaca ttatgacgca acgcggcctg gcggaagggc gcggactctt
      481 caactggcac atcggcaacc agcaccgaat cgacgccgac tttttcgcac tgggcataaa
      541 actcatcaat gcctttgtta aacaccagat tggcatacat caacaggcca atgggaatgg
      601 tcgggtgttt ctggcgaatc agtgccagca tttcaaaaca ttgtgccgga gtcacacctg
      661 ccgcaaaagc gcgcagagtg gcgttttgaa tcgtcgggcc atccgccagt gggtcggaga
      721 aggggatacc taactccagc gcgtcagcac cggcttcaat tagcgtatcg ataattttca
      781 atgactgctc aatgcccgga tcaccgagcg tgacgaaagg aacgaatgcg ccttctttgc
      841 gctccttcaa ctgggcaaac agagattcgt agcgttccat cagatttccc ctcgtgcttt
      901 caaaatatcg tgaacggtga agatgtcttt atcgccgcga ccggaaaggt taaccaccag
      961 tagctgctct ttatccgggt tttcgcgcat cattttcaac gcatgggcca gggcgtggga
     1021 ggattccagc gccgggatga tcccttcgtg caggcacagc gttttgaagg cttcaagggc
     1081 ttcatcatcg gtaatagaca cgtaatcagc gcgtccagtg ctgttaagat acgcgtgttg
     1141 tgggccgaca gacgggaaat ccagtccggc ggagatggag taagattctt caatctgccc
     1201 gtcttcggtt tgcatcatcg gcgctttcat accgaaatag atacccacgc gaccatgttt
     1261 tagcggtgcg ccgtgctcgc cagtttcgat accgtgacca cctggctcca caccaatcag
     1321 gccgacgttg gtttcattga tgaaatcagc aaacatgccg atggcattcg aaccgccgcc
     1381 aacacaggcg ataacggcat ccggcaggcg accttctctt tccagaatct gcgctttggt
     1441 ttcttcgcca atcatccgct gaaactcacg cacaatggtc ggataaggat gcgggccagc
     1501 tgcggtgccc agcatatagt gcgcggtttc gtaactaccg gaccagtcgc gcagcgcctc
     1561 gttacaggca tctttcagcg tcgcggaacc gctatgcacc gggatcactt ccgcacccat
     1621 taagcgcata cgaaaaacgt taggcgactg gcgttcaacg tctttggcac ccatataaat
     1681 acggcatttc aggccgagca gggcgctggc aagggccgac gccacgccat gctgaccggc
     1741 accggtttcg gcgatgattt cggttttacc catccgcttc gccagcaacg cctgccccag
     1801 cacctggtta gttttatgcg cgccgccgtg cagcaaatct tcacgcttga gatacagcgt
     1861 ggtgttcgtc ccggctgtaa tgttctggca tttggtcagc gcggttggac gcccggcata
     1921 gtttttcagc aggtcgttga actgagcctg aaattcagga tctttttgcg cactgacaaa
     1981 agcttcttcc agctggcgca gagcaggcat caggatttgt ggcacgtaca tgccgccaaa
     2041 ctcaccaaaa taggggttaa gtaatgttgt cattgttcct ttccttaata tgcgcgcagc
     2101 gtctggaaaa ccgaggccaa aagacgtgcg tctttgatgc ccggttgcga ctctacagca
     2161 gaattaaaat caagtccggc gcagccggtt tgtgccgctt ccacgcagtt atctgcgcct
     2221 aagccccccg ccagcagaac gttgccaagc gattgaccat ttaatagtga ccagtcaaaa
     2281 cgttgcccgc ttccaccctg gccgttgtct aaaacatatt tatcaacgtg ctgaaactcg
     2341 cgggcgggca gggtttcacc gacgcttaat gctttccaga tggcaacatg tgctggcaga
     2401 gcttcacgca gcgtatcgat atacagctgt tcttcattac catgcagttg cactgccgcc
     2461 agcgataaca ccttagcttt gtccaccaca tcggcaatat cgtgattgcg gaacacgcca
     2521 acatactgca acggtgccgc agccatcact tcctgcgcct gttcaacgtt gacgcaacgc
     2581 ggtgatgtcg caacaaaaat caacccaccg taaatcgcgc ccgcgtcata agctgcttta
     2641 gcatcttgcc cacgcgtcag gccacatact ttattctcac ccagcaacac ccggcgcacg
     2701 gcggcgtgca aatcgtcatg ggccatcaac gccgaaccaa tcagaaaacc gttagcgaag
     2761 tggcttaact cgcgcacctg agcgtaagta ttgatgccgg attcgctgat taccgttacg
     2821 ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga gatcaatcga caaatcacgc
     2881 agatcgcggt tgttgatgcc aacgaccttt gcccctaatg caatggcgcg ctccagttcc
     2941 tcttcattac tgacttcggt cagcaccccc atctccagac tgtgagcgac ggcggcaagc
     3001 tggcgatatt ggtcgtcatc cagtactgaa agcattaata agcaggcatc ggcctggtaa
     3061 tagcgcgcca gatagatctg gtaagggtcg ataatgaagt ctttacataa aatcggctgc
     3121 ggggcgattt ggctgacgat ggggaggaaa tcaaagctcc cctgaaaata tttctcatca
     3181 gtcagcaccg aaattgccga agcgtaatgt ttataaatgg cggcaatgcg tgccggatcg
     3241 aaatcatcac ggatcacgcc ttttgacggc gacgctttct tgcactccag aataaacgcc
     3301 gtgcgtgcac cctgtagcgc atcataaaaa tgtcgcgtgc tcggctgaac ctcattctga
     3361 aaactggcca gcggttgctg ctgtttgcgg gtttctaccc aaatcgcctt gtctgcgacg
     3421 attttcgcta aaacggtttg catcatttac cctcgtgccg ccagcgcggt aactctgtcg
     3481 taagcggaac cactgcgcag tacctcaaga acggtttgcg cattggcttg cagatcttca
     3541 tggccatgca ggcgcattaa catggcgacg ttcgcagcga cggctgcttc atgggcggcg
     3601 tcgcctttac cttgtaacaa acgtgttaaa atgtcacggt tttcttccgg tgttccgcct
     3661 gccagttgct cctggtggta gggtgtcagg ccaaaatctt cagcggtcaa ttgatagctc
     3721 ttaatttcgc cgtcatgcag ctcggcaacg attgtcggcg cgtgtaatga aacttcatcc
     3781 atcccgccgc tgtgcaccac cgccgcgcgt tgatacccca gcacgcgcaa ggtttcggca
     3841 atcggcagca ccagttccgg actataaaca ccaattaacg ccagcggcgg atgcgccggg
     3901 ttaatcaatg gccccagcac attgaacagg gtgcgggttt ttagttgctg gcgaaccggc
     3961 attgcatggc gaaatccggt gtgatatttc ggtgcaaaga ggaaacatac gcctaactca
     4021 tccagcgcct ggcgcgattt atcggcgttc atatcaagat taataccgaa cgccgccagc
     4081 agatcggacg aaccagattt actggagacg ctacggttgc cgtgtttcgc cactttcagc
     4141 ccacaggccg cggcgacaaa cgcactggcg gtagaaatat tgatactgtt gctgccgtca
     4201 ccgccagtac cgacgatatc agcaaacaga taatccgggc gcgggaacgg cgctgcgttt
     4261 tccagtagcg cggttgctgc cccggcgatc tcgttcgggt gctcaccgcg aattttcatg
     4321 ctcaccagcg ccgccgccag ttgttccggc ttcagctcgc cacgcaccac cgctgaaaac
     4381 agctggtggc tttcttgttg gctaagcgtc tgcgcctgat acagtttttc cagaatcggt
     4441 tgcagcgtgt tggctggctc tagtttctgc tgcgcccagg ccagcgtttg ttccagcagg
     4501 cgagcgccct gggtggtgag aatggattcc ggatggaact ggaatccaca aacgcgatcc
     4561 gcatcgtgac gtactgccat caccatgcca ttaaaatggg cgttgatggt taaaccggcc
     4621 ggaatgttac tgccaaccag cgagtgataa cgcgccaccg gcagcgggtt tgttaatccg
     4681 gcaaacatcg cctgaccgtc atgttcaatg ctcgacgctt taccgtgaag aatttcgccc
     4741 gcctgaccga catagccccc gtaagcttcg acaattgcct gatgtccgag gcaaatgcca
     4801 ataatgggca gcttgccacg caagcgggtg aggagttccg gcatacaacc ggcttcgctc
     4861 ggcacaccgg ggccaggaga aagcatcagt accggattgc tcatggtcgc caggcgttca
     4921 attaaggtct gcgccggaat atggttgcgg taaatcacca cgttatgccc attgctgcgc
     4981 aactgatctg ccaggttgta cgtaaaagag tcgatattat cgagcagcag tatgtcagcc
     5041 atcagaaagt ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca gcgcgggctt
     5101 tattacgggt ttcgtcggct tccgactgcg gaatagaatc aaggactacg ccagcaccgg
     5161 cttgcacggt ggcgataccg ttttccacca gcgccgagcg gatcacaatg caggtgtcga
     5221 gatcgccgtg cgcggtgaaa taacctaccg cgccgccgta gctgccgcgg cgacgacctt
     5281 ccgcctcggc aattaactgc atggcgcgca ctttcggcgc accgcttaac gtccccatat
     5341 tcatacaggc gcggtaagcg tgtagggcgt cgagatcgtg gcgcagttca ccgaccacgc
     5401 gggagaccag gtgcatcacg taagagtaac ggtcaacttt ggtgaggtcg gcgacgtagc
     5461 ggctgccggg ggtgcaaatg cgtgccagat cattacgggc aagatccacc agcatcagat
     5521 gttcagaaag ctctttatga tcggtacgca tttccagttc gatgcggctg tcgaggtctc
     5581 tgtccagcga gccatcggca cgacgaccgc gtggacgtgt tccggcaatc gggtagatct
     5641 caatctggcg gctggtggcg tcatacttga gcgaactttc cggcgacgcg ccaaacaggg
     5701 tgaaatcatt atcctgcata aaaaacatgt acgggctggg attactcttt ttcagcacgt
     5761 aataggccgc cagcggtgac gggcagggca gagagaaacg gcgagatggc accacctgga
     5821 aaatttctcc agcgcgaatc gctttttgca acaaacgcac tacgccaccg aactcttcat
     5881 cgctctgatt acattcacaa cgcatatgcg gcacggaaac caccggcagc ggcggcgcgg
     5941 cttcggtcag ttgctgacgt agttcgttca ggcgagcagt gagacgttgt ttttcttctt
     6001 cattcggagc aaacaggctg gcctgaatac gggtgctttt tttctgatgg tcaatcacca
     6061 tcagagtttc agcgagataa aaacagaaat cagggcagtt attttccgct gacagttgcg
     6121 gtaaatcttc aaatcccgcc acaaggtcat aagagaacag gccgccgaag aacattgctt
     6181 ctcgttcttc cttcggtaca ttcaacagat tctgcaataa acggaaagcg tcaaaaaccg
     6241 aaagggagca taagcgagcg tcttcatcca gcagtggact gacagggggg aagcgcagca
     6301 cacggcagtt tggtaattgt tcattttcca cacccgcagg cagggcgtta tccagtagtg
     6361 tcagcagggc ttcgccgttg ccggaaagtg cctggattgt gacagtgtca cctaaagctg
     6421 taatgcgcag cgcactgtct accagcagca ggctttttaa atcatctttg ctgtcgatat
     6481 ctgcggattc cagcagcagc gttgccggac gatccccaca caactggtga aaaagcgcgg
     6541 tgggattgtc gcgataagcg ccttcgcagg ttagcagttc gagagtcggt ttttgtgttt
     6601 gcattgttat tctctaattt tgttcaaaaa aaagcccgct cattaggcgg gctgggtatc
     6661 tgattgcttt acgcatggtg aatacactgc ccgtttcagg aagtgcgcca ccaacctttc
     6721 agtacgaaaa ttgctttcat tgtcgatacc ctttttacgt gaacttgcgt actagttaac
     6781 tagttcgatg attaattgtc aacagctcat ttcagaatat ttgccagaac cgttatgatg
     6841 tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga attatgcagt
     6901 gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc cagaagcatt
     6961 ggtgcaccgt gcagtcgaga tgcgcgtcgg caccctggcg atcaccgacc atgacaccac
     7021 agctgcaatc gcgccagcaa gagaggaaat ttcacgttcg ggactggcgt tgaatcttat
     7081 tcccggcgtg gaaatttcca cggtctggga aaatcatgaa attcatattg tcgggctgaa
     7141 tattgatatt actcatccgc taatgtgtga gttcctcgcg cagcagacag aacggcgcaa
     7201 tcagcgggcg cagctgattg ccgaaagact tgaaaaagcg caaatacctg gggcgctgga
     7261 aggcgcacaa cgactggcgc agggcggcgc agtgacgcgc ggtcattttg cgcgtttcct
     7321 tgttgagtgc ggcaaagcca gttcaatggc ggatgtcttt aaaaagtatc tggcgcgcgg
     7381 gaaaaccgga tacgttccgc cacagtggtg tacaatagaa caagctattg atgtcattca
     7441 tcattctggc ggtaaggcgg tgctagctca tcctgggcgg tacaatctta gtgctaaatg
     7501 gctgaaaaga ttggttgcgc attttgccga acaccacggt gacgcgatgg aagtcgcgca
     7561 gtgccagcaa tcgcccaatg aacgcaccca gctggcgaca cttgcgcgtc agcatcattt
     7621 atgggcatca caaggatctg attttcatca gccatgcccg tggatcgaac tgggtcgtaa
     7681 actctggctg cccgcaggcg ttaaaggcgt ctggcagcta tgggaacagc cgcagaacac
     7741 cacagagagg gaattatgag ccagtttttt tatattcatc ctgataaccc gcagcaacgt
     7801 ctgatcaacc aggcggtgga gatcgtgcgt aaaggcgggg tgattgttta tccaactgat
     7861 tccggctatg cgctcggctg taaaattgaa gataaaaacg ccatggagcg tatttgtcgt
     7921 attcgccagc tgccggacgg tcacaacttt accctgatgt gtcgcgatct ttctgaactc
     7981 tcgacctatt catttgtcga taacgttgcg tttcgtttaa tgaaaaacaa cacgccgggc
     8041 aactatacct tcatcctgaa ggggacgaag gaggtgccac gccgtctgtt gcaggaaaaa
     8101 cgcaaaacca tcggtatgcg tgtgccttct aacccgattg cgcaggcgtt gcttgaagca
     8161 ctgggcgaac cgatgctttc cacttcgcta atgctgccag gcagcgaatt taccgaatcc
     8221 gatcccgaag aaattaaaga ccgtctggaa aaacaggtag atttgattat tcacggcggc
     8281 tatctcgggc agaaaccaac aacggttatt gatcttaccg atgatacgcc ggttgtggtg
     8341 cgtgaaggcg taggtgatgt gaagcctttc ttataagtgc gtaactctgt atactacgcg
     8401 gccttaaaat ggcgctatac ggcgccattg attctcctgt tcgacgcctg tgaaggcgac
     8461 actaaaagga agctctatga gcgaaaaatt acagaaagtg ctggcccgcg ctggccacgg
     8521 ctcccgccgt gaaatcgaat ctattattga agccggtcgt gtgagtgttg atggcaaaat
     8581 tgctaaactc ggcgatcgcg ttgaagttac ccctgggctg aaaattcgta tcgatggtca
     8641 tctgatttcg gtacgtgagt ccgctgaaca aatttgtcgc gttctggcct attacaaacc
     8701 ggaaggtgag ttgtgtaccc gtaacgatcc ggaagggcgt ccgacggtgt ttgaccgtct
     8761 gccaaagctg cgtggcgcac gctggattgc cgtaggtcgt ctggacgtta acacctgtgg
     8821 tctgctgctg ttcaccaccg atggtgaact ggcaaaccgt ttaatgcatc caagccgtga
     8881 agttgaacgt gaatacgccg tgcgtgtatt tggtcaggtt gacgacgcga aactgcgtga
     8941 tttgagtcgt ggcgtgcaac tggaagatgg tccggcagct tttaaaacca tcaagttcag
     9001 cggcggcgaa gggatcaacc agtggtacaa cgtgactctg accgaaggcc gtaaccgtga
     9061 agttcgtcgt ctgtgggaag cggttggtgt gcaggtgagc cgcctgatcc gtgttcgtta
     9121 cggtgatatc ccactgccaa aaggtctgcc acgcggtggc tggacggagc tggacctcgc
     9181 ccagactaac tatctgcgcg aactggtgga gctaccgcca gaaaccagct ctaaagtcgc
     9241 tgtagaaaaa gaccgtcgtc gcatgaaggc gaatcagatt cgtcgtgcgg tgaaacgtca
     9301 cagtcaggtg agcggcggtc gtcgttctgg cgggcgtaat aacggttaat caaagtatgc
     9361 cggacgtcat atccggcatt tttacagatt aataatcaat ccctatttgc gccttaacac
     9421 cggcatcaaa cgcatgtttg atggggcgca gctcacttac cgtatccgcc agttcaagaa
     9481 tatcccgatg acagcctcga cccgtgataa tcaccgtctg ttgatgtgga cgttcattta
     9541 acgcctgcac cacttcttcc agtggcaaat agtcatacgc caccatatac gtcagttcat
     9601 caagcaaaac catatccagt gaggaatcag caagcatccg ctttgcatgt tgccagactt
     9661 cgcggcaggc ggcggtatca gactcgcggt tttgtgtatc ccaggtaaag cccgttgcca
     9721 tcacctgaaa ctcaacgcca tgtggctcca gcagattgcg ttcgccatta ggccaggtgc
     9781 ctttaataaa ctgcacgacg cccacttttt ttccgtgacc aactgcgcgt gttgccgtac
     9841 caaatgccgc ggtggttttg ccttttccat tgccggtaaa gacgatgata ataccgcgtt
     9901 catcctgggc ctgggccaca cgagcatcta ctttttcttt cactcgctgc tgacgctgtt
     9961 ggtagcgttc atcactcatt gggaaattcc tggtttacgg cccggttggg cgtcaaaggt
    10021 catgccggtt ttacggcggc tgtcatcgcc catcagccag aggtagagcg gcatgatatc
    10081 agcgggtgtt ttaagtttct gtggatcttc ggtcgggaag gcgcttgcgc gcattgcggt
    10141 gcgcgtaccg cctgggttaa tgcagttgac gcgcaggcgc tgctgatatt catcggccag
    10201 tacctgcatc atcccttcgg tggcaaattt cgacgctgca tatgcacccc agttagctcg
    10261 tccctgacgt ccaacgcttg atgaagtaaa gaccagtgaa ccggcgtccg atttgagtaa
    10321 taaaggaagc agtgcctggg tgagcataaa ggtggcatta acgttgacct gcatgacgtc
    10381 ctgccagacc tgcggatttt gttcgctcat tgggcaaata tcgccgagca atccggcatt
    10441 atgcaaaaca ccatccagac gcggataatt aacgacaatg cgctgtgcca gttgttggca
    10501 atcttcggac gtgcaggtca gcaaatcgag aataaaccac tgtggctgac gtccagtttc
    10561 ttcgtttatg tggctggcta cctgacgtaa tttttcttca ttacggccca acagaatcac
    10621 tgtcgcacca tagcgtgcat acgtcatcgc ggcttcacgg ccaatcccat cgctggctcc
    10681 cgtcaccagg ataatgcgat cattgagtaa atcttgtttt ggctggtaat gcatggctac
    10741 tcctcaacga cgttgtctgt cgcgctgcca gtgtacttta tgactttggg gctttatgcc
    10801 tgaaacagaa gggcatttca atcagcctgg ggcgataaca ccgcaaaatg gatactttgt
    10861 catactttcg ctgcaataac atctctgcga gacggcttaa catgcctgtt gtaaactgtg
    10921 agccaaagcg ttgtttaacc aaggtgggga ctcgtggaat tgttgtctga atatggtttg
    10981 tttttggcga aaatcgttac cgttgtgcta gcgattgcgg cgattgccgc cattattgtc
    11041 aatgttgctc aacgtaataa acgccagcgt ggcgagttac gggtcaacaa tctcagcgaa
    11101 cagtataagg agatgaaaga agaactggcc gcggcgctga tggactcaca tcagcaaaaa
    11161 cagtggcaca aagcgcagaa gaaaaagcac aagcaagaag cgaaagcagc aaaagcgaaa
    11221 gccaaactgg gcgaggtggc aactgacagt aaacctcgcg tctgggtgct ggattttaaa
    11281 ggcagcatgg acgcccatga agtgaactcg ctacgtgaag agataacggc tgtactcgca
    11341 gcattcaaac cgcaggatca ggttgtgcta cgtctggaaa gccctggtgg catggtgcat
    11401 ggttacgggt tggcggcttc gcagctgcag cgtctgcgtg ataaaaacat tcctttaact
    11461 gttacggtag acaaagtcgc tgccagcggc ggttacatga tggcctgtgt ggcggacaaa
    11521 attgtttccg caccgtttgc tattgtgggt tccattgggg tggtggcgca aatgcccaac
    11581 tttaaccgct tcctgaaaag caaagatatt gatatcgaac tgcacactgc cgggcagtat
    11641 aagcgtactc tgacgttgct gggtgaaaat accgaagaag ggcgggagaa attccgcgaa
    11701 gagttgaacg aaacgcatca gttgtttaaa gattttgtga agcgtatgcg tccgtctctg
    11761 gatattgaac aggtggcaac gggtgaacac tggtacggac aacaggcggt agagaaaggc
    11821 ctggttgatg aaatcaatac cagtgatgaa gttattctta gcctgatgga aggccgtgaa
    11881 gtggttaatg tacgctatat gcagcgtaaa cgactcattg accgattcac cggcagcgcg
    11941 gcagagagcg ccgatcgatt gttgctacgc tggtggcagc ggggtcaaaa gccattgatg
    12001 taaaagacaa acgcgaggct aagacctcgc gttttgcttt aatcaaccag atgatatttt
    12061 tctgaaagca catgggccag gtgtttgaac atattaaaca ccgcggtgct tttggctgtt
    12121 ggcaatcctt gttcatctaa aaagtagtcg ccggtaaata ccagcacgcc attacgctgc
    12181 gtgacgccgg tggcttcaat ccctgcgagc gtatcctcat gctcacgaat gattttgttg
    12241 gcctctttca acagcgtttc gcggtcgatg ggttgtgtct ctttgttcat tatttactcc
    12301 ttaaacaagg acattagtct acgccaggca tggcttgcag acaaatatac cacgctggtg
    12361 gcaagagcgc cttactggca actttggatt ttgcatgcta ataaagttgc gtatcggatt
    12421 ttatcaggta cagtgtgacg ctttcgtcaa tctggcaata gatttgcttg acattcgacc
    12481 aaaattccgt cgtgctatag cgcctgtagg ccaagacctg ttaactcagt cacctgaatt
    12541 ttcgtgaaca gagtcacgac aaggggttga tatccgcaga gaacgagtcc atatcggtaa
    12601 ctcgttgcca gtggaaggtt tatcaacgtg cgacgcattc ctggaagaat caaattaggt
    12661 aaggtgaata tgggtaaagc tcttgtcatc gttgagtccc cggcaaaagc caaaacgatc
    12721 aacaagtatc tgggtagtga ctacgtggtg aaatccagcg tcggtcacat ccgcgatttg
    12781 ccgaccagtg gctcagctgc caaaaagagt gccgactcta cctccaccaa gacggctaaa
    12841 aagcctaaaa aggatgaacg tggcgctctc gtcaaccgta tgggggttga cccgtggcac
    12901 aattgggagg cgcactatga agtgttgcct ggtaaagaga aggtcgtctc tgaactgaaa
    12961 caactggctg aaaaagccga ccacatctat ctcgcaaccg accttgaccg cgaaggggaa
    13021 gccattgcat ggcacctgcg ggaagtgatt gggggtgatg atgcgcgcta tagccgagtg
    13081 gtgtttaacg aaattactaa aaacgcgatc cgccaggcat ttaataaacc gggtgagctg
    13141 aatattgatc gtgttaatgc ccagcaggcg cgtcgcttta tggaccgcgt ggtggggtat
    13201 atggtttcgc cgctgctatg gaaaaagatc gctcgtggtc tgtctgccgg tcgtgtgcag
    13261 tcggtggcgg ttcgcctggt ggtcgagcgt gagcgtgaaa ttaaagcgtt cgtgccggaa
    13321 gagttctggg aagtcgatgc cagcacgacc acgccatctg gtgaagcgtt ggcgttacag
    13381 gtgactcatc agaacgacaa accgttccgt ccggtcaaca aagaacaaac tcaggctgcg
    13441 gtaagtctgc tggaaaaagc gcgctacagc gtgctggaac gtgaagacaa accgacaacc
    13501 agtaaacctg gcgctccttt tattacctct acgctgcaac aagctgccag cacccgtctt
    13561 ggatttggcg tgaaaaaaac catgatgatg gcgcagcgtt tgtatgaagc aggctatatc
    13621 acttacatgc gtaccgactc cactaacctg agtcaggacg cggtaaatat ggttcgcggt
    13681 tatatcagcg ataattttgg taagaaatat ctgccggaaa gtccgaatca gtacgccagc
    13741 aaagaaaact cacaggaagc gcacgaagcg attcgtcctt ctgacgtcaa tgtgatggcg
    13801 gaatcgctga aggatatgga agcagatgcg cagaaactgt accagttaat ctggcgtcag
    13861 ttcgtagcct gccagatgac cccagcgaaa tatgactcca cgacgctgac cgttggtgcg
    13921 ggcgatttcc gcctgaaagc acgcggtcgt attttgcgtt ttgatggctg gacaaaagtg
    13981 atgcctgcgt tgcgtaaagg cgatgaagat cgcatcttac cagcagttaa taaaggcgat
    14041 gctctgacgc tcgttgaact tacaccagcc cagcacttta ccaagccgcc agcccgtttc
    14101 agtgaagcat cgctggttaa agagctggaa aaacgcggta tcggtcgtcc gtctacctat
    14161 gcgtcgatca tttcgaccat tcaagatcgt ggctacgtgc gagtagaaaa tcgtcgtttc
    14221 tatgcggaaa aaatgggcga aatcgtcacc gatcgccttg aagaaaattt ccgcgagtta
    14281 atgaactacg actttaccgc gcagatggaa aacagcctcg accaggtggc aaatcacgaa
    14341 gcagagtgga aagctgtact ggatcacttc ttctcggatt tcacccagca gttagataaa
    14401 gctgaaaaag atccggaaga gggtggtatg cgcccgaacc agatggttct gaccagcatt
    14461 gactgcccga cttgtggtcg caaaatgggg attcgcacag cgagcaccgg ggtattcctt
    14521 ggctgttctg gttatgcgct gccgccgaaa gagcgttgca aaaccaccat taacctggtg
    14581 ccggaaaacg aagtgctgaa cgtactggaa ggcgaagacg ctgaaaccaa cgcgctgcgc
    14641 gcaaaacgtc gttgcccgaa atgcggcacg gcgatggaca gctatctcat cgatccgaaa
    14701 cgtaagttgc atgtctgtgg taataaccca acctgcgacg gttacgagat cgaagagggc
    14761 gaattccgca ttaaaggtta tgacggcccg atcgttgagt gtgaaaaatg tggctctgaa
    14821 atgcacctga aaatggggcg tttcggtaaa tacatggcct gcaccaacga agagtgtaaa
    14881 aacacacgta agattttacg taacggcgaa gtggcaccac cgaaagaaga tccggtgcca
    14941 ttacctgagc tgccgtgcga aaaatcagat gcttatttcg tgctgcgtga cggtgctgcc
    15001 ggtgtgttcc tggctgccaa cactttcccg aaatcgcgtg aaacgcgtgc gccactggtg
    15061 gaagagcttt atcgcttccg cgaccgtctg ccggaaaaac tgcgttatct ggccgatgcg
    15121 ccacagcagg atccggaagg taataagacc atggttcgct ttagccgtaa aaccaaacag
    15181 caatatgtct cttcggaaaa agacggaaag gcgactggct ggtcagcatt ttatgttgat
    15241 ggcaaatggg ttgaaggaaa aaaataacct ttaattctgt caggttttta taaacaaagg
    15301 gtcgcgaaag cggccctttt ttattgcata ttattttttc ttcacaccta tacactaagg
    15361 ctataaatga tatagtggtt atagttagca ccttttttat tattaaatcg tattagtcac
    15421 ccgccaggtg tgacgaaaaa acgatgttct gatggcgtct aagtggatgg tttaacatga
    15481 aattacaaca acttcgctat attgttgagg tggtcaatca taacctgaat gtctcatcaa
    15541 cagcggaagg actttacaca tcacaacccg ggatcagtaa acaagtcaga atgctggaag
    15601 acgagctagg cattcaaatt ttttcccgaa gcggcaagca cctgacgcag gtaacgccag
    15661 caggacaaga aataattcgt atcgctcgcg aagtcctgtc gaaagtcgat gccataaaat
    15721 cggttgccgg agagcacacc tggccggata aaggctcgct gtatatcgcc accacgcata
    15781 cccaggcacg ctacgcatta ccaaacgtca tcaaaggctt tattgagcgt tatcctcgcg
    15841 tttctttgca tatgcaccag ggctcgccga cacaaattgc tgatgccgtc tctaaaggca
    15901 atgccgattt cgctatcgcc acagaagcgc tgcatctgta tgaagattta gtgatgttac
    15961 cgtgctacca ctggaatcgg gctattgtag tcactccgga tcacccgctg gcaggcaaaa
    16021 aagccattac cattgaagaa ctggcgcaat atccgttggt gacatatacc ttcggcttta
    16081 ccggacgttc agaactggat actgccttta accgcgcagg gttaacgccg cgtatcgtct
    16141 tcacggcaac ggatgctgac gtcattaaaa cttacgtccg gttagggctg ggggtagggg
    16201 tcattgccag catggcggtg gatccggtcg ccgatcccga ccttgtgcgt gttgatgctc
    16261 acgatatctt cagccacagt acaaccaaaa ttggttttcg ccgtagtact ttcttgcgca
    16321 gttatatgta tgatttcatt cagcgttttg caccgcattt aacgcgtgat gtcgttgatg
    16381 cggctgtcgc attgcgctct aatgaagaaa ttgaggtcat gtttaaagat ataaaactgc
    16441 cggaaaaata atttcttgcg ttattttcgg caccttttat gtagcgaagg tgccggaata
    16501 tattctc
//
LOCUS       DAZGXZ010000113        16262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000113 DAZGXZ010000000
VERSION     DAZGXZ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.113"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..691
                     /locus_tag="ACHKJF_003867"
     CDS             <1..691
                     /locus_tag="ACHKJF_003867"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0951138.1"
                     /translation="MTFRNSSKLSNFISKCKCGDPIKSEDRVPGPYFYYGASGPIDTH
                     TEFNFNGKYLIIAQDGSIGCANVADGKFWANNHVWVLKVKDEYDIESICRFLDKHFPC
                     WKGVTTGSVVPKVTSENLLNILIPIDIAKNREIGSKLRLAVTTAAYAKKLTASAKTLV
                     ESLIEGQLTEQQLIQAQQALEDGDSSFDQAILSKLSAEGYAIEGATPLFSDVDELYSL
                     LEEAAQAEAEE"
     gene            694..1995
                     /locus_tag="ACHKJF_003868"
     CDS             694..1995
                     /locus_tag="ACHKJF_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951139.1"
                     /translation="METRGTDSSTTRQYNDPVNSEAALNLCLQLWQQGGLNANKAALL
                     LAAVPALRSLLQPIIQPKKNDAETDIVSAFSLTAPLLDAFNDLSQSGEWQLALLGLNP
                     DVRQHWINLAAARCQEAGTMSDTMVLVKLIQQLGNASEWVLAQLESTATTPQIIAGPL
                     AQTERDLLGHSLNDNAAIPALCRILRTSYTLFTVSEQNEPPAPIQAVDVTAKQLTNNW
                     CSGRLLALPNTLLDEHNLKPNADWLLVSRSGHDNMPLTELFAQQPWLFLLSLIIFVQD
                     AWAAEQRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPL
                     YPALDANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGEAGQYRIHTSFSDACYSLP
                     LAPLFGYKSQTLQRTIKQLAQNCYANKKRAANRINLQGSSL"
     gene            1992..6278
                     /locus_tag="ACHKJF_003869"
     CDS             1992..6278
                     /locus_tag="ACHKJF_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951140.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQNKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMAYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSSNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PNDAESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            6289..7137
                     /locus_tag="ACHKJF_003870"
     CDS             6289..7137
                     /locus_tag="ACHKJF_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951141.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSPPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            7140..8297
                     /locus_tag="ACHKJF_003871"
     CDS             7140..8297
                     /locus_tag="ACHKJF_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951142.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            8287..9972
                     /locus_tag="ACHKJF_003872"
     CDS             8287..9972
                     /locus_tag="ACHKJF_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFP0951143.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRYADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            9980..10522
                     /locus_tag="ACHKJF_003873"
     CDS             9980..10522
                     /locus_tag="ACHKJF_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951144.1"
                     /translation="MPEVAWVNETFPLLASGKLLDQDVLVHSLGCSVWNTFGHEQSFM
                     AVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAKGQQKLEEKLKNLLEA
                     AQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGTQVIAAPDVEVVFSRF
                     LFIKNLNMIVLDRIHYEVLM"
     gene            10519..11988
                     /locus_tag="ACHKJF_003874"
     CDS             10519..11988
                     /locus_tag="ACHKJF_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFP0951145.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(12188..13453)
                     /locus_tag="ACHKJF_003875"
     CDS             complement(12188..13453)
                     /locus_tag="ACHKJF_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0951146.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            complement(14166..14630)
                     /locus_tag="ACHKJF_003876"
     CDS             complement(14166..14630)
                     /locus_tag="ACHKJF_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0951147.1"
                     /translation="MKKTLRIQFGERVKELRIATGLSQEAFADRCGFARSYMSRIERG
                     GSNASLDAIEVLANALSVEPWQLLASGSSEDNDPELLVPYAADGSCFHPGLASTRDGS
                     FGVGDKAAQKRFGTFSEALEYLRSMETAKWRRPNASGNWGIVSAVRWDKLRK"
     gene            14871..15368
                     /locus_tag="ACHKJF_003877"
     CDS             14871..15368
                     /locus_tag="ACHKJF_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951148.1"
                     /translation="MAGSGFLLPMVAGVALSCVSYGFVARIVSKHQISTLRAVSWGIL
                     GTLSLVLGACAFSAVSAEHPAAKHKTLCLGSAPECYAKAHHNPVKECKQVMDKKAAFR
                     HVWQESEARPVFDTYLWHNEQKKTIQAFGQQAKAINSMNMLIPLQYFCVFNANTGEVI
                     AASFE"
     gene            complement(15409..>16262)
                     /locus_tag="ACHKJF_003878"
     CDS             complement(15409..>16262)
                     /locus_tag="ACHKJF_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005185975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0951149.1"
                     /translation="KKDLDEEFSKLGVSHIKTVLKPRVSKGKVFYSLLLDIPVSNKVD
                     LILSEGEQRAVSLASFLAELKLANHSCGIIFDDPVSSLDHHRRRRVATRLVEEAKNRQ
                     VIILTHDIAFLSELIFATEKNNVDSLIHHLQWTGDFSGCVYDGLPWDKVSYKTRVEKL
                     KQESRQLDPWPVYPSAEQDNSMRRLYSRMRSTVEKMVEDVIFAGIVVRFSEIIGVGNL
                     HKLSGLERECCIAISELWSKCHRITDAHDQPAYKQTALPSPDEFRADLELILDLAKKA
                     AATQIKK"
BASE COUNT         4546 a         3703 c         3834 g         4179 t
ORIGIN      
        1 aatgaccttt aggaattcat caaaactatc taatttcatt agtaaatgca aatgtggcga
       61 ccctattaaa tcggaagaca gagtacccgg accatatttt tactatggcg catcaggtcc
      121 aatagatact cataccgaat tcaatttcaa cggtaaatat ctgattattg cgcaagatgg
      181 ctctattggc tgtgcaaatg tagctgatgg aaagttttgg gcaaacaacc atgtttgggt
      241 tttaaaggta aaagatgaat atgatattga gtctatttgt aggttcctgg ataaacactt
      301 tccgtgttgg aaaggtgtga ctactggcag cgttgtacca aaagtgactt cagaaaacct
      361 gttaaatatt ctgattccga ttgacatagc gaagaataga gaaataggaa gtaagctgag
      421 actagccgtt actacggcag catatgcaaa aaagttaaca gcatctgcca aaactctagt
      481 tgagtcatta atcgaaggcc aactgactga gcaacaacta atccaggccc aacaagccct
      541 ggaagatggc gacagcagct ttgatcaagc gattttaagc aaactgagcg ctgagggtta
      601 tgccattgaa ggtgccacac cgctatttag cgatgtggat gagttataca gtttgctgga
      661 agaggcggcg caagcggagg ctgaagagta aagatggaga caaggggcac ggacagcagt
      721 acaaccaggc aatataacga ccccgtaaat agcgaagcgg cacttaacct ctgcctgcag
      781 ctttggcaac aaggtggttt gaacgccaac aaagcagcat tgttattggc ggctgtgcct
      841 gcccttaggt cactgctaca accaataata cagccgaaaa agaacgacgc tgaaacggat
      901 atcgttagcg cctttagcct cactgcacca ttgcttgatg cctttaacga tttaagccag
      961 tcaggtgaat ggcaattagc gttgttgggc ttaaacccag atgttcgcca acattggatc
     1021 aatttagccg ccgctcgctg ccaggaagca ggcacgatga gtgacacaat ggtattggtt
     1081 aaactcattc agcaactggg caatgccagt gaatgggtct tagcccagct ggaaagcact
     1141 gccaccacgc cgcaaatcat tgccggccct ctggcacaaa ccgaacgcga tttgctcggt
     1201 catagcctaa acgacaatgc tgccattccc gcgctatgtc gtattttgcg caccagctat
     1261 accttgttta ccgtgagcga acaaaatgaa ccaccggcac ctatacaagc ggtggatgtc
     1321 accgcaaaac aactgacaaa taactggtgt agtgggcggt tgttagcact ccctaatacc
     1381 ttgcttgatg aacataacct taaacccaat gccgattggt tattggtgtc ccgctcgggt
     1441 catgacaaca tgccgcttac cgagctattc gcccaacagc cctggttatt tttactcagt
     1501 ctaattatct ttgtgcaaga tgcctgggct gccgagcaac gtggcggttt gttgctcacc
     1561 ttacctgccg ggcaaaatgc ctttgccccc ggtcaaataa atgtggcggt acagggcatt
     1621 gaaggtgatg aggtgagtct gggtagtttg gccgagtttc ttgtgcttct gctcggtgag
     1681 ctcaatatac ccctctaccc ggcactcgat gcgaacacgg aaagcatcaa ccgcctaaac
     1741 cgcgtcctga gctcattcat tgccgaattg ttggcaaaaa aaatctggca atttaccgag
     1801 gcgggtcgtg gagaggccgg ccaatatcga attcatacaa gcttcagcga tgcctgttat
     1861 agcctgccgt tagcaccact atttggctac aaaagccaaa cgttacaacg gaccattaag
     1921 caactggcgc aaaactgcta tgccaataaa aaacgcgccg ccaatagaat aaaccttcaa
     1981 ggatcgtcgt tatgagtttt gaattaaacg aacagatggt tgatagcctg gcaaaacaac
     2041 atcgctgggc ggcgacgggc gtatcagatt atccacctcg cgacccgtta gtgggccaga
     2101 gccgcttttt taaacgcttt caaacctttt tgcatacggt ggatcacgac gatgatcgct
     2161 ttgcccatgt gtttgccgtc gaagccgaat ggggtcgggg taaatcacgt ttaggccatg
     2221 aattggtcgc acaaattaac gactgttcaa ggggctggtt tgtccgcgac gaacaaggcc
     2281 aactgcacga taagcagctg tttaatcgcg acacgcaaga caaatacctg gcgctctaca
     2341 ttcgctactc gcaagtggca tcagattatc aaaactcgga taactggttt gcttttggtt
     2401 tatatcaggc gctattacca ctggccaccc aaacctttga tggatcgatt caaagtgaaa
     2461 ttgccaaaca agcctatgaa cgcttaatgc catttggctt taaatctgaa ctgttagcag
     2521 atgccttaca gctgaacgcc aaccacagcg aagaagacct ctatactgac caaacgctgg
     2581 tagtcagttt ggtgcaagcc gcctataaag tattaaagag ctttggcgtg cagtatatgt
     2641 tggtggtgct ggatgagctg gaaaccgttg cagaagccgc cacctttggc ctggaagatg
     2701 aacaaaataa acgcctcgat ggccaggcca ttcgcctgat tggtaaagca attaaagagg
     2761 aagaccctcg ccgtaaactt ccatggttgc gctatgtagc gctgtgttca ccactgcttg
     2821 gccagcaatt gcgcgaaatt caatcggtcg cacgccgctt tgagttagtg gaacttgagc
     2881 ataatgcctt tgccgatgta tcagactatg tggcaagcct cttaaaagac ggcaagctgg
     2941 gttttaatta ccctgctggt ttagtggaag ctgcatacgc catgagtggt gccaacttcg
     3001 gttggtttaa cgtggtcatg gctaatgtgg atgcagtgct gagccaatac caacaagcag
     3061 gccgcagtat caaggatgtg ggcgatttat ttgaagctgt gctggcaggc tctggccgcg
     3121 tagccaggca tgtgctggat aaaggcgcga tagaaggcat tcaaactcgc gaccaggcac
     3181 tattgaccga atgtcgtagt ttgctctacg gccaattacc cgtgcgctta gctgaaatgg
     3241 cctatgccac agaattgctg gcgctgaaaa acgaagatct ggaacccata gccgctcatt
     3301 accgcaaact gagcattgac cgcctacagt gccgacaagc cctggagcaa gccaaattcc
     3361 gccgcgataa agatgaatgg tactaccctg cagtagatca agcgctgaac ttagatactt
     3421 tgctggataa cctgcgtact ttttcaatta aagaacaagc cgcaagcggc gataaaggcg
     3481 cggtattact acctctgtca aaaggtgagt ttaaatactt actcagcttg ctgtatgacc
     3541 acccagcgat tgaattcgct gccgatgcct tatggcacaa gctagtgggt gaaactaccg
     3601 aactggacgc cagcgaagca acgcatattg gccccagtgt ggcgatgtta ctgcgcctgg
     3661 atttgcgcta tcgcagtgcc cagcaaaact cactgatttt taaagatccc gccatgggcg
     3721 acgctcatga acaggcaatg agtgaactta gcaaacaaag tagccagaag ccgctcatca
     3781 aggcattagc ccgtgtaacg ggctttgtga gattacttga taacaactgg acctatgatg
     3841 aaaacttgct gagtaataaa gcgggcagcg acgatgcacc tttggccatt ttaaccgagc
     3901 cccgtggccg ccagggtggt ttacttaccc tggatggatt taagcttcac ccagaaggta
     3961 aagccttgtt tgcctgggtc agcaacctaa ttgagctgaa caatcttcac acctttgcgg
     4021 caaaccacag ccaccaaaat ggtcgcacgc cagtattagc gttaactgct tctgcacatc
     4081 tcatggagca atacagccga ctggatgaaa gaaatgagtt gcgtgatgcg atactgctgc
     4141 attatgtcaa cccaagcgaa gcagaccagt tagagcgtat tggcttaagt ttggcagcat
     4201 gtcagttgca cggcgtgaat ttaactgccg acagctttac cgcgaaattc aaaaacaagc
     4261 tgcatgcctt gacaaccttt gccacggacg ccattcacaa gtggcgccag cgtttgcagc
     4321 agcgcggttt aattgcctgg ccgttaaagg tagatggtaa attatcatcg aatgaccgag
     4381 acctgttgtt taaaggctgg tatcagctgg ccattgctca ccctgagtta aatggcattc
     4441 tcgatttaca gcagcaacac ggcgtgccag tgaatgagct gagcagcttg ttggataaac
     4501 ttaaggtgcc gggcagctat attgccaaag gctacacggc cgatgaacat gccgggctat
     4561 ttactgaact taataatgtg cagcgaagcc aggcgcagat cccattattt ttagcccgta
     4621 ttgctcaccc taataaaaag cataagtggc aattcgaagg gtttaagcaa cagttttatt
     4681 tcgcctatgt ggctgaaacg agtgttaccg ccaaaggcgt gtttaatgac tggatgtggt
     4741 ggtgtggaga gctgaactta ctcactttaa ccaatccaac agaaaaacag gcaatttggg
     4801 agcactaccc gcgctcccgc ttagaaaatg ccattagaga agcgcaaaac tggttccgcg
     4861 gtaacgatat gggcagctat gcagccaatg tggaagtcat gagccgcgtg tatggttatg
     4921 cacgcattaa tgaaatgttc gcgcctctgg gtaaaaataa gctgggtttc gtcaccgtgg
     4981 aagctaaaga gcagctggaa aaagcacaat cgctatttaa cgtgctcaaa cagcaagaag
     5041 aacaactggc tgatatggtc gaggccaacg acacgaaagt attggcaggg cttatccaca
     5101 aacgcgctga agtgctggag ctggtagcaa aggttaaacc gcttaatagt tccaggccta
     5161 tgctaaaaga tgcccatatt ctcagcttag aagacaaaac gacctcgctt tatcaacgga
     5221 ttgaacaggc ctgtttattt gccgagtttg ttgagtgcag cgctgagcgt atcaacaacc
     5281 gactcgttga tttaatcata gatgttgaaa cagagtgtgc cccgctgacg aattttccga
     5341 aacgtctgta caccaacacg ctgcgcacca taggtcatat tttagatgga gcgttaaaag
     5401 acgataccag cagtgccact ggccgaaaag agcagcaagc agatagcgat accttactgc
     5461 attacctgcg taaactcgat ttaggtagag cgcatgacaa gctttcagcc ttagcgcaag
     5521 aagtgggttt aaacctgcac aatgatcaac agttgccgat agcggagatt caggggcata
     5581 ttttgtccag ctaccgcaac tgcaaagaac gatttagtaa gctggttaat aacctcacag
     5641 agcaaaaact acgtgcacaa caactgcaag aatggcttag tagcgccacg gctgagtatc
     5701 agttcgccga tgacattgca gagctaccaa aactggtaat gaaattacag ctcattgaag
     5761 atgctattgc cgatctcccc aacgatgccg agagcaagcg tcaaagtatg caaaacagtt
     5821 tgcgcaatgg gcagttttca tcgttacggg atttaccaga agagctttta aaaccggcac
     5881 gcggtcaact tacaccgatt caaggccagc tacttaaaat tgaagagcgt ctaaaccaag
     5941 taagacgcaa ctccatcgca caggtaaaca gctggctgcc gttattaaaa ccgctattgg
     6001 cttctcaaaa gcaagcggaa ccagcagcgt tgactctgga ggatgtgagc aatttgggtg
     6061 tcagagaact tcaacaggtg tgtgaaagca cccaggccaa atggcagagt caaggtgagc
     6121 aaatattaaa tgatacaggc ttaacactgg cggattggca accgatttat aaggctttga
     6181 gccaaaatca ggaacccgtg ttaacacctg agcaacagaa aggacttgta gacaaaggca
     6241 ttgtaaaaat gcgccttacc tttgccactg gtttgtagag gtgacgcgat gagtcgttat
     6301 ttatctgcgg cactggccag taatcgtaaa ggccgttttt tgcaaactgt tgcgggggct
     6361 acccccttaa tgaaggattg gattagctcg ccgccagcca gcggtttgtt aatagtgcaa
     6421 gcagaagagt tgactgacgc taatactatg cagcacctgt atcactgggc tatgcaggca
     6481 ggttgtgccg ctttggtaat aaacctgaaa gcagaacaat ttaccttatt agcgcaattg
     6541 ccctatccac tggattggca gttagtacca gcgagcttga gggggcagga accggggcta
     6601 accgctttgc tagccagtga aacagaccag gctattgctg gctttactgg cagcgcagat
     6661 cgctaccagc accaggccgg tgatgtagtg catacccgct acatacgaaa acacagcaac
     6721 agtgggctat tagcctttac cacgttacct ttgtggtcgc tgactttact cgatcatagt
     6781 gagctcctgg tgagctggtt gaattggttt gtggaccatg caggcattgc tgaacgaata
     6841 atcgaaccta aagcgccatc aactgactac acgccagata agcacgatct ggttgtattg
     6901 ctattactct acgctggcgg tggcatgaac ttacaagcgt tgtctgagca taatgcggtt
     6961 aagctgatgt ttgatgttaa tagccttgat attgtcaagc ggggtgagat gttgcggcag
     7021 cacgatttta tcgacgacgc gggcataaca gctaccggta aaacatgcct acaagcctcg
     7081 caatattggg cttatgcccc attacttggc gagcagctac acacaggaac gctataagca
     7141 tgaatgagaa caaaacgatg aatttagcgc aatcgctcgc catcgatagc cagcttcgct
     7201 taccgggaag tgcaggacgc ttgcttaaac aggtccgtga tattgagcaa gtggcgccgc
     7261 tattccgtga tgctggctta gtgaaagcgg tccccaagcc cacaattgtt aatttacgcg
     7321 tagccggtat tgcgactaaa tcgtgcttag acaaggtatt gggtggtttt atttatgcca
     7381 gtgcagcagt gcgcacagat ttgttaatag atgacaataa agtcagcacg gtgggtagcg
     7441 actcggtatc agaactggat gatattgatt ttgaagctca gcgtaaacgg cttgatttaa
     7501 ttgaaattcg tcaggcttat cagttagttg agaaaaaact tttaggttat gagcctggcg
     7561 atttaatttt gctggatacg ccgttgtttt tagcacggga tatggctccg ctggagagaa
     7621 atgtccgtca taaacaagag tacgaaaaaa ccagacaagt gatagaaact ttttggcaaa
     7681 accatcgctc cagaattttt ccctggaatc aaaatggtgt agtacttgcg agcattttgg
     7741 ctgagcgctt tagtgccatt gtaagtatcg ccaaacaaga tttacgtacc gaacaaggac
     7801 gcaaacaaat tttagcatct gatgggtttt ctaaagagtt gatgcaaaat ctggaaggac
     7861 tggatgaaag tttggttggc gtgggagatt tgcgatttat caatggcatt ttaggcaact
     7921 attcgcgcac catcgcattt cgtttgtctg aacaagaaaa gcgcattgaa cccaatattg
     7981 aagctaagca aggtctaatt ggttttcact tccgtagtgc ccgaggtgga cagataaaaa
     8041 tggtgcaatt agcgggtgac gaaccagaat ggaaaagtgc tgatctcgat ttagttgcct
     8101 ctcgcctgat ggtgctggat atgcaaaaca aaggtaatgc aatgccgttg ccacaattac
     8161 ttggttatca acagcttggt attttaccta aatttgctac gttttatcgc cagggtttac
     8221 accaggcact aaagaataat gatattgagg cagggtggtt ggcaggcctc gatgagggat
     8281 tgaataatgg cttataaagt tttaggtaaa ttagtcggta acactggtga tgccagtcag
     8341 ttaactatgg ttgtacaaga ctcgttttca gtacgccgtg gtgagtttgt acgcattatg
     8401 caccaggaac gcaaagatga ggggcaagtc gccgtactgg ggcgagtgac caagataagc
     8461 cgtactaata tgctctataa cgccggcttt ggcgatggtg taaccgagct tgagcttttg
     8521 cccggtgcga atgtaactgg tgagaatatg tttgctcagg tcgagctggt tggttaccgc
     8581 gatcctgtta ctcgtcaaat caaaattcca cgtcgcccgc ttaacccggg taccgctgtt
     8641 gaaaccgtcg attttcagtt tttgagtgat ttttacgaat ttaatgaaca ctccagtttg
     8701 cacttaggta acttagtagg ctacgacaaa ggtgagaata cagttcctgt ttttattgac
     8761 gtgaacaagt tggtcactga gcatttggct gttctggcaa tgacgggttc aggtaagtct
     8821 tatacagttg gccgcattat tgagcgttta gttgcggtta ataacggtac ggtagtagta
     8881 tttgacccac atggtgaata cggtaaagcc ctcgctggcg gtaatctgca attctcaagc
     8941 ttcttagaag ccactgacga taagcgtgat caagaagcac tgccactgat aaaacagacc
     9001 tttgaaaagc tgcaagccgc cggtgcaggt attcaggttt actcgccgca gcatgaatct
     9061 tttaaacata aatacgccag taagaatacg ccattagcgc ttcagtttga ccattttgaa
     9121 atggacgata ttgctgaaat attacctggc ttaacggaac cgcagcaacg agtactcgat
     9181 gttgcaattc gttattggcg ctatgcagat aagactgagc cacgagatat taaccgccta
     9241 cgcttcttat taggtgatgg tattgaagag ttaaaagagt gggacgacct ttctgaagct
     9301 gaggcgaagg cgttatcagg ccgtagtgcc gccgtcgcct cgatgaagct ttcacgtgtg
     9361 cttaatgaag ctaaaagttt ttacagcgca acaatggctg agccaactga tatttacaaa
     9421 atggtgggtc gcccgagcaa tcaacagggg cgtttagtcg ttgttgactt acaaggtttg
     9481 agtgataccg ctaagcaagt catttgtgca ctgttatcta gtgaaatatt aaaagcagcc
     9541 tcgagcaaaa cggacccgct gcgcccatgc tttatcattt acgaagaggg tcataatttt
     9601 gccccggccg gcggcaacgc agtcagtcat cgcattatta aaaagatcgc tggtgaaggt
     9661 cgtaaatttg gtgttggatt tggcattgtg agccagcgtc catcgaaatt agattcagat
     9721 gtgacctctc agtgcaatac gctaattacg atgcgcttaa agaaccctga cgatcaacga
     9781 tttatagcta aagcatcaga tatggtgagc aaagctgatc tggaggaact cccgagttta
     9841 tcaaccggtg aagctttggt gtgtggtcgt tcaatccctg caccactgct tgttaaagtt
     9901 ggtagtaagg cattaattca cggtggtgaa tcaccagagg tactgcgtgt ttggggctct
     9961 ttcagtggct aaggtgccta tgcctgaagt tgcctgggta aatgagacat ttccactttt
    10021 agcaagcggt aagctattgg accaggatgt attggtccac agcctgggct gtagcgtttg
    10081 gaatacattc gggcatgaac agtcatttat ggcggtagtg gaatgcccgg ctccagggac
    10141 atttggtgct gatattcgct ctgactcggg ctggtttcat aaatcttctg catcgcctgt
    10201 gtgtttaatc gaatttgaac gctttgatgg ttcagcgaag gggcagcaaa agctggaaga
    10261 aaaattaaaa aacttactgg aagcagcaca gcgctggaat cattgcccca aaaccctggt
    10321 cctcagtgcc tggagccagg gcttagtagg tgtacctgat actcaaaaac tgaaagacat
    10381 atgccgtatg ggttttactt cgtccacggg tacccaggta atcgccgctc ctgatgttga
    10441 ggtggtattt agtcgctttt tatttatcaa aaacctgaac atgattgttt tggacagaat
    10501 tcactatgag gtgttgatgt gaatataaaa tcagcacatt ttttgccagg gacgaataaa
    10561 cgccttggtg acaaatacct gttacttgaa tgccttggtg atggcagcca tggctgggta
    10621 tggcgggcgg aacgcctgaa agatggaaaa attgtcgcag taaaaatccc taaggatatt
    10681 actcgggaag accgtcagct tgccgaaggt aaagagcttt taaacgtcga gccgcacgaa
    10741 aatattgtgc aaatttttga tatgggccgt atagataatg aatggttcta tattgaaatg
    10801 gagtattttc ccagccaaac cctggcgcaa aagctcgatg acaggcagcg taattttggc
    10861 cagacctatg aacggttgtt cagaatatat cgtcaagtgc tctgtgctgt gcattatttg
    10921 gccgaattac cagtaccaat atctcatgga gatattaagc cacataacat tttggttgga
    10981 gagcgggacc tggtgaagtt gacggacttt ggcagctcag ctttaccaga agaaatttac
    11041 gttcgtaccc gtgaaaatgg cgggactgtg ctgtattcgg ccccagagtt ctccaacgtc
    11101 gatagccgta gaggcagcct ggaagagcta ctattgggcg acatctatag cttaggtgtt
    11161 ttgctatacc agctgctgac aggaaagctg ccccatgata cgccagcaca agttcagcgc
    11221 cacgcacctt tcaagcttcc gacagagatt aatagctcta ttcatacgga cctggaacaa
    11281 gtggttctta cttgtttaca aaaacgagcg gaggatcgct tttccactat ttccgactta
    11341 attcatgcat ttgattctgc tgccactaag caattaaaag ttggtgctat tgctcccgtt
    11401 tttcaaacta aacctgctca ggactggtca agtgcggtac ttgaagcaat gtccgatcaa
    11461 tcttaccaaa aagctgctca attagcgtcg caggagtata gccgcagtaa agatctacaa
    11521 gctttacatc agcaacttat tgccttgtat cgcgccaatc gcttattcga ctttgaaaaa
    11581 gttgtagaag ataataaagc gatcttactt gagggtaaat tgactcaacc agcagctttg
    11641 ttcgagttga tagttaaggc aaacctacag ttacgcaata tcagtcaagc aaatttctgg
    11701 ctgttgcaac gaaagcaagc tgaagcagaa aacgcggcca ccatgtattt agaatcatct
    11761 atccttggcc ttgaagcgaa atattctgaa gcaagagtac tattggagaa agtaaaccaa
    11821 acaactccaa tgaagttcca tgtgttaagc caactcattt tagtttgcga gcaaatgcgt
    11881 gattactctg gtgcagcggc ttatttgaaa gcggcattgc gagtagcacc attggataac
    11941 agcatgaaag agaaaaaaga attgtatgca aagcttggag ttatttgatt gaaaggatga
    12001 ccattgcgcg aacaaggcat agtggatatt taacacttcc gttcacatac cggacaaaaa
    12061 aataaagttt tacataacga tttccttctt gattgagtta agacaaagcc tctccccatt
    12121 agggcggagg cttctcagtt ataagccttt aattaaaaaa agttaatatt catatactta
    12181 gctagaatta tcgaaaagaa ctattaatct ttgcatagtc aaatggagtg atagccgcca
    12241 ttctattcgc atccagataa tcagcccacc actgcagcat caacattcgc tccccaagat
    12301 gttccgcctt atggatataa gccgcccgta ctgagctacg ttcctgatga ctcatctgcc
    12361 gctctactgc atccctcgac cacaatcccg attcaatcaa agaactacac gccatcgtcc
    12421 tgaaaccatg tccacacact tccgttttcg tgtcatagcc catcactcgc aaggccttgt
    12481 taagcgtatt ctcactcatt ggcttacgcg gatcgtgatc accaacgaag atcagctcac
    12541 ggtttccact catgcttttg attttttcca gaatggtgag agcttgtcga gataagggta
    12601 caagatgtgg agtccgcatc ttcgaaccac gctgagaatg cttaactccc tccagtggct
    12661 cacgttctcc cggaatcgtc cacatggcag tatcaaaatc aacttctgac cagcgagcaa
    12721 aacgcaactc gcttgaacgg atgaaaacta acaatgtgag ttcaaccgcc agtcgggtta
    12781 acggcctgcc agaatagtga tagatgcgtt gaagcaattc aggaatgcgg ttaagttcca
    12841 gagcagcacg atgctgtctt ttcgccgtag ctaccgcacc agcaatctct tgagcggggt
    12901 tgtagtcgat taatccactc tgtacagcaa agcgtataat cgcggtagta tgctgctgca
    12961 aacgagcagc gacttcgaga cgcccagacg actccaccgc tttgatgggg acaagcagat
    13021 cccgagtctt cagatcagca atgttccgct taccgatagc agcaaagaga ttatcttcga
    13081 ggcttttcaa tacacgagcg ctatgcgatg cagaccactt ctgattgctg gcgtgccact
    13141 ctctggctac cacttcaaat gttatcacct cttgctcatg ctctacctta acagctttct
    13201 tgttctcact aggatcgacg ccgttagcta acagcttacg ggcttcatcc cggcgacctc
    13261 tggcatcgac cagcgagact tcagggtatt tccccagcgc cagcatcttc tccttaccgc
    13321 caaaacgata acgaagtcgc cagtatttgg agccattagg gtgaaccaac aaaaccatgc
    13381 cttcaccgtc agtaagttta taggcttttg cttccggctt agccgaacgg accttcacat
    13441 cactcagagc catgatgagt atcctttcaa gggccctgtg tgggtacaaa cataatcgaa
    13501 ccgggaaata cccgcagttg tacccgcatc agtaagttga tgtagattga atcaggttga
    13561 cttaggttga gtgaaaaagc gaggaaagcc ttgcggatac tggatttcag gcataaaaaa
    13621 agacctcagt tgaggtctat ttacatactt ttggtgccga aggccggact cgaacctaag
    13681 tctccataac ctgctgtaat cacgttccct ttagccttta aaagaacaat cgtaggacct
    13741 ttttgttcta ggggctacat cttaagtttg atccggtgga cggaatcaaa ccgagtgttc
    13801 gtgagggcac aaaacatcaa cgtgccatac ctgccttttg atgtttatgg aggtctgccg
    13861 ggtcgggagg aaagaacgtt tgtcagctaa gtgggcgtta agaaaacgcc actgcgttcg
    13921 gattttgaaa caagctaaac ccggcgggcc ttgttcataa aaaccattta actcaatgtt
    13981 attgatatga attaatccat tcagcgtcaa attgaaacgc cactgcaacg cctacgtaac
    14041 gcacccgcat tttgctgttt tattcactct tatagaaaca aagggctgat gaaacataag
    14101 actatcagca tttaatgaat gctgttcatc catacagcat aaaaattcat aaacttacat
    14161 ttgaactact ttctgagttt gtcccaccgc actgccgaaa caatgcccca gttgccactg
    14221 gcattgggcc tacgccattt tgccgtctcc atactacgga gatactcaag cgcttcggaa
    14281 aaggtgccaa aacgcttctg agccgcttta tcacctacac caaacgaacc atcacgggtg
    14341 ctcgccaaac cggggtgaaa acaggaacca tcggccgcgt atggaaccaa cagttccggg
    14401 tcgttatctt cagatgagcc agacgctaat aactgccacg gctcaacgct caaagcgtta
    14461 gccagcacct caatcgcgtc cagagacgca ttagagccac cacgttcaat tctgctcata
    14521 tagctacgtg caaacccaca ccgatcagca aaggcttcct ggctcaatcc ggtggctatg
    14581 cggagttctt ttacccgctc gccaaactgt attctcaagg tctttttcat gcactgaatg
    14641 tgcagttttg accactgtaa ggccacgctc tacatgagac attttgaatc aatcctaact
    14701 tcagcgggct gagtcactcg cattagccgc ctgcctcatg acaaccctta atgattcata
    14761 atgaacatca atagtgacat tttaaatcca caaagtatta tgatcgtcat cggttggtcg
    14821 tccccaacat tcgctagtag gcgtgatgtg tttttgaaaa tgagggaatc atggcgggat
    14881 caggtttttt attaccaatg gtggcgggtg tcgcgcttag ttgcgtcagc tatggatttg
    14941 ttgctcgcat tgtttcaaaa catcagatca gcacgctacg cgcagtttca tggggaattc
    15001 ttggaaccct ctcattggtg ctaggcgcat gtgcattttc cgctgtaagc gcagaacacc
    15061 cggcagcgaa acacaagacc ttgtgcttag gtagcgcccc ggaatgctat gcaaaagcgc
    15121 accacaaccc cgtcaaagag tgcaagcagg taatggacaa gaaagcagcg tttcgccatg
    15181 tgtggcagga aagtgaagcg cggccagttt tcgacactta cctttggcac aacgagcaaa
    15241 agaaaacgat tcaagcattt ggacaacagg ctaaggccat taacagcatg aatatgctga
    15301 taccattgca atacttttgt gtttttaacg ccaacactgg ggaagtcatc gcggcttcat
    15361 ttgaatgaaa gcattcaggg aattcttccc tgaatgctta aagattaact attttttaat
    15421 ctgagtcgct gctgcttttt ttgctaaatc taaaataagt tctaaatctg cccgaaattc
    15481 atcagggctt ggtaacgctg tctgtttgta tgcaggttga tcatgagcat cagtaatacg
    15541 atggcatttt gaccagagtt cagaaatcgc aatacaacat tctcgctcaa gcccacttaa
    15601 tttatgtaaa ttgccaacgc ctataatttc actgaatcta accacaatcc cagcgaatat
    15661 cacatcttca accattttct caaccgttga tctcatacgg ctatataatc ttcgcatcga
    15721 gttgtcttgc tcagcactgg gatatacagg ccaaggatct aattgccgag attcttgttt
    15781 taacttttca actctggttt tatagctgac cttgtcccaa ggtaaaccat cataaacgca
    15841 gcctgaaaaa tccccagtcc attgcaggtg atgtattaag ctatcgacat tatttttctc
    15901 ggtagcaaat attaactcgc tcaaaaaagc gatatcatgc gtgaggatta ttacctgccg
    15961 attttttgct tcttcaacca atcttgttgc tacccgtctt cttctatgat gatcaagaga
    16021 agatactgga tcatcaaaaa tgatcccaca tgaatggttg gcaagtttta attcagctaa
    16081 aaaagatgct aacgatacag ccctctgttc cccttcactc aaaattaaat ccaccttatt
    16141 tgatacaggt atatccaaaa gcaaactata aaaaaccttc cccttagaaa ctcttggttt
    16201 gagaactgtt tttatatgag aaacacccaa tttagaaaat tcttcatcca aatctttttt
    16261 aa
//
LOCUS       DAZGXZ010000114        15993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000114 DAZGXZ010000000
VERSION     DAZGXZ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.114"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(9..824)
                     /gene="djlA"
                     /locus_tag="ACHKJF_003879"
     CDS             complement(9..824)
                     /gene="djlA"
                     /locus_tag="ACHKJF_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP0951150.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            1079..3433
                     /gene="lptD"
                     /locus_tag="ACHKJF_003880"
     CDS             1079..3433
                     /gene="lptD"
                     /locus_tag="ACHKJF_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP0951151.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDAHGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            3486..4772
                     /gene="surA"
                     /locus_tag="ACHKJF_003881"
     CDS             3486..4772
                     /gene="surA"
                     /locus_tag="ACHKJF_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP0951152.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            4772..5761
                     /gene="pdxA"
                     /locus_tag="ACHKJF_003882"
     CDS             4772..5761
                     /gene="pdxA"
                     /locus_tag="ACHKJF_003882"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP0951153.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADTTLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLGVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            5758..6579
                     /gene="rsmA"
                     /locus_tag="ACHKJF_003883"
     CDS             5758..6579
                     /gene="rsmA"
                     /locus_tag="ACHKJF_003883"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP0951154.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            6582..6959
                     /gene="apaG"
                     /locus_tag="ACHKJF_003884"
     CDS             6582..6959
                     /gene="apaG"
                     /locus_tag="ACHKJF_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP0951155.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            6966..7808
                     /gene="apaH"
                     /locus_tag="ACHKJF_003885"
     CDS             6966..7808
                     /gene="apaH"
                     /locus_tag="ACHKJF_003885"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP0951156.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(7886..8365)
                     /gene="folA"
                     /locus_tag="ACHKJF_003886"
     CDS             complement(7886..8365)
                     /gene="folA"
                     /locus_tag="ACHKJF_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP0951157.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(8558..10420)
                     /gene="kefC"
                     /locus_tag="ACHKJF_003887"
     CDS             complement(8558..10420)
                     /gene="kefC"
                     /locus_tag="ACHKJF_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP0951158.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(10413..10943)
                     /gene="kefF"
                     /locus_tag="ACHKJF_003888"
     CDS             complement(10413..10943)
                     /gene="kefF"
                     /locus_tag="ACHKJF_003888"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP0951159.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSVPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(11051..12382)
                     /locus_tag="ACHKJF_003889"
     CDS             complement(11051..12382)
                     /locus_tag="ACHKJF_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951160.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRYFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(12440..12727)
                     /gene="fixX"
                     /locus_tag="ACHKJF_003890"
     CDS             complement(12440..12727)
                     /gene="fixX"
                     /locus_tag="ACHKJF_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP0951161.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(12724..14010)
                     /locus_tag="ACHKJF_003891"
     CDS             complement(12724..14010)
                     /locus_tag="ACHKJF_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0951162.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(14061..15002)
                     /locus_tag="ACHKJF_003892"
     CDS             complement(14061..15002)
                     /locus_tag="ACHKJF_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0951163.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGMFDAAQPDAS
                     RTGETHTMEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKDNIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(15017..15787)
                     /locus_tag="ACHKJF_003893"
     CDS             complement(15017..15787)
                     /locus_tag="ACHKJF_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP0951164.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVIAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         3927 a         4485 c         4014 g         3567 t
ORIGIN      
        1 tttaaaactt atttaaaccc tttctgctgc tttatcagct catatgcctg ctgaatttcc
       61 tgcgctttct gcttcgccat ctccatcatc tcaggcggca aacctttcgc caccagctta
      121 tcgggatggt gttcactcat cagcttacgg taggcacgtt tgatggtggt cgcatcatcc
      181 gtcggcttca cgcccagcac attacaggca tcttccagcg tcgggccacg ctgcgcttgc
      241 tgccagttac caccgccaga ttgctgctga taaccgccgc caaactgtgc accgccctgc
      301 atcatgcgca aaaactggtc aaactgggcg cgggagatcc ctaattcttc agcaatgaca
      361 tacagcaccg cccgttcatt cgggtgcagt gaaccatcag caaacgccgc ctgaatctgg
      421 atctccagaa acatacgaat taagtcaaaa cgaccaaagc agacactgcg aaactggcgc
      481 atcttttcgc gcagcgggta attgtctgat tttcccaccc ggaacgcatt ttgcgccgca
      541 gtacgggaag cgccatgaag attcattcgg tccatcaact ggctagcgat atgaatatca
      601 gcctccgtga cgcgaccttt ggatttggtt aaatgcccca tcacttcaaa agtggtggca
      661 aaaaacagcg cctgacgctc acgctggttg gcgaaccacg ccattttacg gctacgggct
      721 ttatcaaaca tatggccaat taacaggcct aacactacgc cccaaaagcc gccgcccatc
      781 agtaaggcca cggccacgcc aatgattttt ccccaatact gcatatattc cccaaatcga
      841 cacacggata tcagggctat ctcccacaat ataaaggtgc ttttaccgtt ttccggctgc
      901 ggtcaattgt gggacatcgc ctataatttg cattatcata cctgtcattc aatgccgtgc
      961 ctaacaccac tgacgctatt cggacaggat taacactagc gtagagatga cgagtacgtt
     1021 agtctctgac cgtttgtcac gcgcaacgtt accgatgatg gaacaataaa atcaacgtat
     1081 gaaaaaacgt atccccactc tcctggccac catgattgcc accgcccttt atagtcaaca
     1141 gggactggca gccgacctcg cctcacagtg catgttgggc gtgccaagct atgaccgtcc
     1201 tctggtacag ggcgatacca atgacttacc cgtgactatc aatgctgacc acgcgaaagg
     1261 ggactacccg gatgacgccg tgtttactgg cagcgtggat atcatgcagg gtaacagccg
     1321 tctgcaggcc gacgaagtgc agctccatca aaaagaggca ccaggacaac cggagccggt
     1381 acgtaccgtt gatgcgcacg gtaatgtcca ttacgacgat aaccaggtga tcctcaaagg
     1441 gccgaaaggc tgggcgaatc tgaacaccaa agataccaac gtctgggaag gtgattacca
     1501 gatggtgggt cgccagggtc gcggtaaagc ggacctgatg aaacaacgtg gcgaaaaccg
     1561 ctataccatt ctggataacg gtagctttac ctcctgtctg ccgggttctg acacctggag
     1621 cgtggtaggt agcgaaatta ttcatgaccg cgaagaacaa gttgcggaga tctggaacgc
     1681 ccgctttaag gtgggtccgg taccgatctt ttatagcccc tatttgcagt tgccggtggg
     1741 tgacaaacgt cgctctggtt tcttgatccc gaacgccaag tacaccacca ccaactactt
     1801 tgagttctac ctgccatatt actggaacat cgcgccaaat atggatgcca ccatcacgcc
     1861 gcattatatg catcgtcgtg gcaacatcat gtgggagaac gaattccgct acctctccca
     1921 agcgggcgct ggcttgatgg aactggacta tctgccttca gataaagtct atgaagatga
     1981 acacccgaac gatgacagtt cacgtcgttg gttattctac tggaaccact ccggggtcat
     2041 ggatcaggtg tggcgtttca acgtcgacta caccaaggtc agcgatccta gctacttcaa
     2101 tgatttcgat aacaagtacg gttccagtac tgacggctac gcaacgcaaa aattcagcgt
     2161 tggctatgcg gtgcaaaact tcaatgccac cgtttcaacc aagcagttcc aggttttcag
     2221 cgaacagaac accagtagct actcggcaga gccgcagtta gacgttaatt actaccagaa
     2281 tgatgttggt ccgtttgata cgcgtattta cggccaggca gtgcactttg ttaacaccag
     2341 agacgacatg cctgaagcaa cccgtgttca cctggaaccg accatcaatt tgccgctctc
     2401 taataactgg ggcagcatca ataccgaagc gaagttgctg gcaacccatt atcagcaaac
     2461 caatcttgac tggtataact ccagaaacac gaccaagctg gacgaatccg ttaaccgcgt
     2521 aatgccgcaa ttcaaagttg acggcaaaat ggtctttgaa cgcgatatgg aaatgctggc
     2581 tccgggttat acccaaacgc tggaaccgcg cgcgcagtat ttgtacgtgc cgtatcgcga
     2641 tcagagcgac atctataact acgactcgtc tctgctgcaa tctgactact ctggcctgtt
     2701 ccgggaccgg acttacggcg gtcttgaccg tattgcctcc gctaaccagg tgacgaccgg
     2761 tgtcacatct cgcatatatg atgatgctgc cgttgaacgt tttaatattt ccgttggtca
     2821 aatctactat ttcacggagt ctcgcactgg cgatgacaac ataacatggg agaatgacga
     2881 caaaacgggt tcactggtgt gggcaggcga tacttactgg cgtatctccg agcgttgggg
     2941 attgcgtggc gggattcagt acgatacacg tctggataac gtagcgacca gtaactccag
     3001 cattgaatac cgtcgggatg aagaccgtct ggtacagctg aattaccgtt acgccagccc
     3061 ggaatatatt caggctacgc tgcctaagta ctattccact gctgagcaat ataagaatgg
     3121 tatttcgcag gtaggtgctg tcgccagctg gccaattgcc gatcgttggt ccattgttgg
     3181 ggcctactac tacgacacca atgcgaacaa gcaagccgac tctatgttag gtgtgcaata
     3241 cagctcctgc tgctatgcaa ttcgcgtcgg ttacgagcgg aagctgaacg gttgggataa
     3301 cgataaacaa catgcggtat atgacaacgc aatcggcttt aacatcgaac ttcgcggcct
     3361 gagctccaac tacggtctgg gtacgcaaga gatgctgcgt tcgaacattc tgccgtatca
     3421 aaacactttg tgatttgttg atttaccacg taatccgcag tgcggttaat tgaaatggaa
     3481 aaagtatgaa gaactggaaa acgctgcttc tcggtatcgc catgatcgcg aataccagtt
     3541 tcgctgcccc ccaggtagtc gataaagtcg cagccgtcgt caataacggc gtcgtgctgg
     3601 aaagcgacgt tgatggatta atgcagtcgg taaaactgaa cgctgctcag gcaaggcagc
     3661 aacttcctga tgacgcgacg ctgcgccacc aaatcatgga acgtttgatc atggatcaaa
     3721 tcatcctgca gatggggcag aaaatgggag tgaaaatctc cgatgagcag ctggatcagg
     3781 cgattgctaa catcgcgaaa cagaacaaca tgacgctgga tcagatgcgc agccgtctgg
     3841 cttacgatgg tctgaactac aacacttatc gtaaccagat ccgcaaagag atgattatct
     3901 ctgaagtgcg taacaacgag gtgcgtcgtc gcatcaccat cctgccgcag gaagtcgaat
     3961 ccctggcgca gcaggtgggt aaccaaaacg acgccagcac tgagctgaac ctgagccaca
     4021 tcctgatccc gctgccggaa aacccgacct ctgatcaggt gaacgaagcg gaaagccagg
     4081 cgcgtgccat tgtcgatcag gcgcgtaacg gcgctgattt cggtaagttg gcgattgctc
     4141 attctgccga ccagcaggcg ctgaacggcg gccagatggg ctggggccgt attcaggagt
     4201 tgccggggat cttcgcccag gcattaagca ccgcgaagaa aggcgacatt gttggcccga
     4261 ttcgttccgg cgttggcttc catattctga aagttaacga cctgcgcggc gaaagcaaaa
     4321 atatctcggt gaccgaagtt catgctcgcc atattctgct gaaaccgtcg ccgatcatga
     4381 ctgacgaaca ggcacgcgtg aaactggaac agattgctgc tgatatcaag agtggtaaaa
     4441 cgacttttgc tgccgcagcg aaagagttct ctcaggatcc aggctccgct aaccagggcg
     4501 gtgatctcgg ctgggctaca ccagatattt tcgatccggc cttccgtgac gccctgactc
     4561 gcctgaacaa aggtcaaatg agtgcaccgg ttcactcttc attcggctgg catttaatcg
     4621 aactgctgga tacccgtaat gtcgataaaa ccgacgctgc gcagaaagat cgtgcatacc
     4681 gcatgctgat gaaccgtaag ttctcggaag aagcagcaag ctggatgcag gaacaacgtg
     4741 ccagcgccta cgttaaaatc ctgagcaact aatggttaaa acccaacgtg ttgtgatcac
     4801 tcccggcgag cccgccggga ttggcccgga cttagttgtc cagcttgcac agcgtgagtg
     4861 gccggtcgaa ctggttgttt gtgccgatac cactctcctt accgaccggg cagcgatgct
     4921 cggtttgccg ctcaccctcc gcccttattc ccccaactcc cctgcacaac cgcaaactac
     4981 gggcacatta acgctacttc ctgtcgcgct acgtgaatct gtcactgcgg ggcagttagg
     5041 ggttgaaaat gggcattacg tggtggagac gctggcgcga gcgtgtgatg gctgtctgaa
     5101 cggtgaattt gctgcgctga tcacaggccc cgtgcataaa ggcgtcatta acgacgcagg
     5161 tattccgttt actggtcata ccgagttttt cgaagagcgt tcgcaggcga aaaaagtggt
     5221 gatgatgctg gcgaccgaag aacttcgcgt ggcgctggca acgacgcatt taccgctgcg
     5281 cgatatcgca gatgctatca cccctgcact tttgcacgaa gtgattgcta ttttgcatca
     5341 cgatttgcgg acaaaatttg gtattgccga accgcgcatt ctggtctgcg gactgaatcc
     5401 gcacgcgggc gaaggcggtc atatgggtac ggaagagata gacaccatta ttccggtgct
     5461 cgacgagctg cgggcgcagg ggatgaaact caacgggccg ctgcctgccg ataccctgtt
     5521 tcagccgaaa tatctcgata acgccgacgc cgtgctggcg atgtaccacg atcagggtct
     5581 tcccgtgcta aaataccagg gcttcgggcg cggtgtgaac attacgctgg gcctgccctt
     5641 tattcgcaca tcagtggacc acggcaccgc gcttgaactg gcgggacgtg gcgaagccga
     5701 tgtcggcagt tttattacgg cgcttaatct cgccatcaaa atgattgtta acacccaatg
     5761 aataatcgag tccaccaggg ccacttagcc cgtaaacgct tcgggcaaaa ctttctcaac
     5821 gatcagttcg tgatcgacag cattgtttct gccattaacc cgcaaaaggg ccaggcaatg
     5881 gtcgaaatcg gccccggtct ggcggcattg accgagccgg tcggcgaacg tctggaccag
     5941 ctgacggtca tcgaacttga ccgcgatctg gcggcacgtc tgcaaacgca tccattctta
     6001 ggcccgaaac tgacgattta tcagcaggat gcgatgacct ttaactttgg cgaactggcc
     6061 gagaaaatgg gtcagccgct gcgtgttttc ggcaacctgc cttataacat ctccacgccg
     6121 ttgatgttcc atctgtttag ctatactgat gccattgccg acatgcactt tatgttgcaa
     6181 aaagaggtgg tgaatcgtct ggttgcagga ccgaacagca aagcgtatgg tcgattaagc
     6241 gtcatggcgc aatactattg caacgtgatc ccggtactgg aagttccgcc gtcagccttt
     6301 acaccaccac ccaaagtgga ttccgccgtc gtgcgcctgg ttcctcatgc aacgatgcct
     6361 cacccggtta aagatgttcg tgtgttgagc cgcatcacca ccgaagcctt taaccagcgt
     6421 cgtaaaacca ttcgtaacag cctcggcaac ctgtttagcg tcgaggtgtt aacgggaatg
     6481 gggatcgacc cggcgatgcg agcggaaaat atctctgtcg cgcaatattg ccagatggcg
     6541 aactatctgg cggagaacgc gcctttgcag gagagttaac gatgatcaat tcgccccgag
     6601 tgtgtattca ggttcaaagc gtctacattg aggcacaatc ttcacctgat aatgaacgtt
     6661 acgtttttgc ttataccgta accatacgca atctggggcg agcgccagtg cagttgttgg
     6721 ggcgttactg gctgatcacc aatggcaatg gccgtgaaac cgaagtccag ggcgaaggag
     6781 tggttggcgt ccagccactt atcgcgcctg gcgaagagta ccagtacacc agcggtgcaa
     6841 tcattgaaac cccgctgggc accatgcagg gtcactacga aatgatcgat gaaaatggcg
     6901 tccctttcag catcgacatt cccgtattcc gactcgccgt tcccacactc attcattaaa
     6961 agaatatggc gacatacctt attggcgacg ttcatggttg ttacgatgaa ctgatcgcat
     7021 tgctgcataa agtagaattt acccctggga aagataccct ctggctgacg ggcgatctgg
     7081 tcgcgcgcgg gccgggttcg ctggatgttc tgcgctatgt gaaatcctta ggcgacagcg
     7141 tacgtctggt gctgggcaat cacgatctgc atctgctggc ggtatttgcc gggatcagcc
     7201 gcaataaacc gaaagatcgc ctgacaccgc tgctggaagc gccggatgcc gacgagctgc
     7261 ttaactggct gcgtcgccag cctttgctgc aaatcgacga agagaaaaag ttggtgatgg
     7321 cccacgcagg gatcacgccg cagtgggatc tacagaccgc caaagagtgc gcgcgcgatg
     7381 tagaagcggt gctatcgagt gactcctatc ccttctttct cgacgctatg tacggcgata
     7441 tgccaaataa ctggtcaccg gaattgcggg ggctgggaag actgcgtttt atcaccaacg
     7501 cctttacccg tatgcgtttt tgctttccaa acggtcaact ggatatgtac agcaaagaat
     7561 cgccggaaga agcccctgcc ccactgaaac cgtggtttgc gattcctggc cctgtcgctg
     7621 aagaatacag catcgccttt ggtcactggg catcgctgga aggcaaaggt acgccggaag
     7681 gtatttacgc gctggatacc ggctgctgct ggggtgggtc attaacctgc ctgcgctggg
     7741 aagataagca gtattttgtc cagccgtcga accggcataa ggatttgggc gaagcggcgg
     7801 cgtcttaaac acagccagat ataggaaggc cggataagac gcgaccggcg tcgcatccgg
     7861 cactagccgt aaattctata caaaattacc gccgctccag aatctcaaaa caatagctgt
     7921 gagagttctg cgcatcggcg tcgtggaatt cgctgaatac cgattcccag tcatccggct
     7981 cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt
     8041 tttgcgcttt tggcaggaac tgctcataaa cgcgaccacc gccaatcacc atgatttctg
     8101 gtacgtcacc acacgccgcg atggcttcat ccaccgattt cacccacgtt acgcgatcgt
     8161 ccgtacccgg ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgatgg
     8221 attcccaggt atggcggccc ataatcacgg gtttatttaa ggtgttgcgt ttaaaccagg
     8281 cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataaca cgatctaccg
     8341 ctaacgccgc aatcagacta atcattgatt tatcctggat ataaaaaatt gtcgccacta
     8401 tacgtaaagc ataaaccgtc gtcgactggc gcaaggatga tgttgagtaa aattttacgt
     8461 tctgctggcg agtccacgct ctcccactgg actcgccaca ttacaatgaa acaaaaacaa
     8521 acagttagct gtaaagtgtg atttacgtca ctctttatta ggatgagggt ttcgtttccg
     8581 gttcatccgc catgttgccg gtatgtttcc cttcttcggt tccctgccag ccatgtcgtt
     8641 gaattaatga cagatgttcg cggtcttcgg taatgatctc acttaacatc gcgctggtgc
     8701 gtttatagac cgctgcgcgg gctttggtgt cgttctcaac cattgccatc tcttccacca
     8761 tctgaatatt aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac
     8821 ctaaactttc cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg
     8881 gcttttcaac gcctgcctga cgcaaacgaa tgtagtgatc gacatcgcgg gcgcgggcaa
     8941 taatctgcaa atgcgggaaa tgttctttca ccatctccgt cagttgcagg ttggtttgcg
     9001 gatcgtcaat tgcgttaatc agcacttccg ctttcgccgc tccggcagac tccagtaaat
     9061 ccatccgcgt ggcatcgcca taaaacactt tcataccaaa tttacgcaag gtttcgatat
     9121 ggtccgggtc gtgatcgagc accaccattt tcaccccgct ggagagcagt aaacgtccgg
     9181 tgatctgccc gaaacgaccg aatccggcga taatcacgcg cggctgttct tcgtcgatct
     9241 catcggcttc acgcgcttcc tcagtagaag attgctcaag gcgattgagg atcaccagca
     9301 gaatcggcgt tgctgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg
     9361 gctccagcac attcgccatc tgcgccgcac caaataccac aaaggcaaac tcactaccct
     9421 gccctaacaa caccgcaaac cagcgacgct gtttatttgg cacctgcaac ggtcgggcaa
     9481 tcagccacag catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca
     9541 atgggttttc aatcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc
     9601 ccaacagcaa acctttaaat ggttcgatat cgctttccag cgcatgacgg tattccgagc
     9661 ttgccagcag tacacccgcc agaaacgcgc ccatcgccat cgacaagcca acctcttcca
     9721 gcagcaaacc aaagccaaac acgaggaata acgccacggc actaaacact tcccgcaagc
     9781 cagagcgggc tacaaaacgc agcgccggac gcgtgacata gcgccccagc aataccacca
     9841 gcaccagcgc ccccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg
     9901 cactgctcgc cgccagtagc gggatcatcg ccaccagcgg gatcgccgcg atatcctgga
     9961 acagcagcac cgcaaaggca ctgcgaccca tttgcgtcac catcagattg cgttcattca
    10021 tcgcctgcat ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga
    10081 cctgccagcg cagcccaagt aacatgcaga acagccccag caggccgccg caaatcacca
    10141 tctgcaatgc gccaccgccg aacactgccg cacgcagctt ccacagcctt tgtggatcga
    10201 gttcgaggcc gataataaac agcatcagca ccaccccaat ctcggcaaag tgcagaatag
    10261 attcggcatc ggtcaccagt cgcagccccc acgggccaat aatgcagcca gcgatcaggt
    10321 agccaagtac cgatcccaga ccaagacgta ccgcaatggg tacaatcagc gccgccgatc
    10381 cgaggtagat cagcgcctga accagcgtat ggctatccat gatgggcctc ctgccattcc
    10441 agcagacgct gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag
    10501 gtacagtgca tggcaaacgg tggcagccag ttcagcccgc agtagattgc cgtcgcctgt
    10561 agcggctgcg acagcacatc aaaacccgga tgcgcaccaa tttcaaaatg gctttccccg
    10621 ccgccggtcg tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc gccatgaccg
    10681 taagcccagc cgtgggagaa aactttatcg atccaaagtt tgaggagcgg aggaacgctg
    10741 taccactgca tcgggtgctg ccagacaatc agatcggcgc gagacagcgc ctcctgctcg
    10801 gcggcaatat cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct
    10861 tccagcgtcc ttgcctgttc aagcatccgt ttattcgcat gggaatgatg cggatacgga
    10921 tgcgcataaa ttataagaat cataggtagc ccgcgtgatt acgcctttgt tttcccacaa
    10981 agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa
    11041 tctgaacaaa ttaccccatt ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg
    11101 acatccctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac
    11161 ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaagatc ggcagtgccc
    11221 aggtcgaaac aatggtgcca atacgactta aggacataat cacgcccacg gcagaggcgc
    11281 ggatatccgt cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag
    11341 agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca
    11401 gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc
    11461 gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa
    11521 tcaccacatt ccccagtgcc gcgtttttgc caacccccaa tcccaacaaa ccaacgattt
    11581 gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa
    11641 caaacagaac aaaaggaaaa tagcggcgat taaacagatc gcgaaaacgg gtttgctgcg
    11701 gctgctcttc atcgaaagcc accggttcgc caaacagttt gatcatcatc tcttcgcact
    11761 cttttactcg ccctttgcgt aacagccagc ggggagattc aggcagttcg aatcgaccaa
    11821 tcaaaatcaa caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca
    11881 catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg
    11941 cgataaagct gatggaaaat gcccgctgac gggtactgga gaactcggtg atcattgagg
    12001 tggcaatggg ataatctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt
    12061 cgacggggga tgaaacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa
    12121 tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc gatgtgccaa
    12181 cgaacagccc ggcgagcgtt cccgcgccca gcaagccaat ccagtcagcg tccagtttca
    12241 gcgccggggt cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa
    12301 acggaccacc gcttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt
    12361 caaagtttct ggacggttgc atgggttatt cctgtttttt gcaatgagag tcagtgcggc
    12421 gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt
    12481 attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac
    12541 atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc
    12601 acgctttcac cagcagctcc agcgcctgtt tatcagcatc ggccttcaca acaatgtgcg
    12661 gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag
    12721 aagtcataaa acggtcactc ctttcatgcc atccttgatt agattgatga agcccacttt
    12781 cttaccgtgg cggaggattt tcttgcgcat cagttctggc gcactgccgt caatggtgaa
    12841 caggtcacgc gccacgccca ccgccagttc cgggtagccg ctaaacatgc gtgggttatc
    12901 aaggaacgcc ggtagtttct gatacatacg catatcgcgc agcggaccac tctcaagatg
    12961 ctgacgatat tccgccagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac
    13021 ggtttttgct gcggcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc
    13081 gagattcata cacattccgg cggcatcacc ggcaatcaat acgccgtcac caaccaactc
    13141 cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc
    13201 acctgcgatc agtggtgcaa cggccggatg ttgtttgaaa tcttccagca tttgcggcac
    13261 cgattttttc gcgtcatgca gatgatgcaa accacaaacc agccccagcg acagggtgtt
    13321 ttcattggta taaagaaaac cgccgcccat caggccatcg gtgggtgatc ccgcaaacag
    13381 gcaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtaa
    13441 ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc
    13501 cagcttttcg gcaaggatgg agttcacccc atcagcaagg atcaccgttt tcgcttcaat
    13561 cacatcgcca tcggcttcta caccgacaac tttgccatcg cgctgtacga ggttatcgac
    13621 gcggatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca gccaggcatc
    13681 aaatttactg cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccattgca
    13741 gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag
    13801 gcgttctacc ggggcagatt cggcgaaacc tggaataatg tgttccaggc tgtgggcata
    13861 gagacgcccg ccggtgacgt tcttggcacc tgcggaattg ccacgctcga taactaacac
    13921 ttgcgcacct tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac
    13981 gatgatggcg tcaaagatat cttcggacat aagagctccc tgtcagcggc aggaccaaaa
    14041 tgcagccctg ccagagtgga tcaacgcgct aaagctgctg tcagcgccgg aaggatcttc
    14101 acggcgtcgc caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatcttta
    14161 ttgatggcga aaatggtttg cgatgcatta gcaccaacca tgtgctggat ctgcccggag
    14221 atccccaccg ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt
    14281 tcgtgctcca tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct
    14341 atcgccttac aaagctgttc tgccagcgca atgttatctt tgctgccaat gccgcgaccg
    14401 acgctgacca ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt
    14461 gccgtgcggg taatcgccac agccggagcc tgccactcca tggtgtgcgt ttcgccagtg
    14521 cgtgacgcgt ctggctgagc cgcatcgaac atgccactgc tgatggtcag taccgcatac
    14581 ggcgtggcaa tgcgttcttc gccaatcgcc aggccgccgt aaaccatgtg tttcactgtc
    14641 gctttaccgt cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga
    14701 taacccagtt ttgccgccag taatttgccg cgacgggtat ttggcaacag caccagaccg
    14761 tctgcgccgt gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccga
    14821 tcgtccggtt tgccgtttaa tttccagaca tgattagcgc cgagctggat tgcctgtgcg
    14881 ccgtcggcat cattgaggac aaaggtgttg atttgattag ctaaagcctg cgcaccgttc
    14941 atcagttccg gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc
    15001 atagcatccc ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt
    15061 cttcgccgtc gccttcaatc acgatgcgct gacgttcgcg ctgtttcggc gcggcaacct
    15121 gttgttcaga ccaggcgtcc acggcgttaa aaccgatatc cgccgccgac catacctgga
    15181 ctggcttttt cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat
    15241 cagtggaaac agcaataacc gcaggcagcg gaatgcttaa cgtttctgtt tcatcttcca
    15301 gttcgcgctc aacggtgagg gtatctgccg tcagggagat aattttgctg acgccgttaa
    15361 ctgccggaat attgaggatt tcgcctacca gcaaaccaac ctgctgggca taaaggtcag
    15421 aagaaccatc gccacagagg atcaaatcaa agcctgcttt ctgggcggcg gcagccagtg
    15481 cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt
    15541 catccgggcc gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc
    15601 ccacacttaa ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt
    15661 caatagcgtt cagatcgtat tggcttattt tggcatcggc tttgctgaag tctaatgaac
    15721 catcagcatt attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa
    15781 tcttcattgc atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta
    15841 cagatatcac gccctttaat ttggaaataa aactttaatc accaatattg aaaatgtcac
    15901 acgcagataa atccaacttt caatattgtt aagttcctca ccaatattga aaacatggcg
    15961 tagcaaaaag aaattttcaa tattgcttta tgg
//
LOCUS       DAZGXZ010000115        15813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000115 DAZGXZ010000000
VERSION     DAZGXZ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.115"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..323)
                     /locus_tag="ACHKJF_003894"
     CDS             complement(<1..323)
                     /locus_tag="ACHKJF_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951165.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALN"
     gene            complement(362..1591)
                     /locus_tag="ACHKJF_003895"
     CDS             complement(362..1591)
                     /locus_tag="ACHKJF_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0951166.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDGDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
     gene            complement(1757..2119)
                     /gene="ssrA"
                     /locus_tag="ACHKJF_003896"
     tmRNA           complement(1757..2119)
                     /gene="ssrA"
                     /locus_tag="ACHKJF_003896"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2334..2816)
                     /gene="smpB"
                     /locus_tag="ACHKJF_003897"
     CDS             complement(2334..2816)
                     /gene="smpB"
                     /locus_tag="ACHKJF_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP0951167.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            2987..3424
                     /gene="ratA"
                     /locus_tag="ACHKJF_003898"
     CDS             2987..3424
                     /gene="ratA"
                     /locus_tag="ACHKJF_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP0951168.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            3414..3704
                     /gene="yfjF"
                     /locus_tag="ACHKJF_003899"
     CDS             3414..3704
                     /gene="yfjF"
                     /locus_tag="ACHKJF_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP0951169.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(3766..4107)
                     /gene="bamE"
                     /locus_tag="ACHKJF_003900"
     CDS             complement(3766..4107)
                     /gene="bamE"
                     /locus_tag="ACHKJF_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0951170.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(4256..5917)
                     /gene="recN"
                     /locus_tag="ACHKJF_003901"
     CDS             complement(4256..5917)
                     /gene="recN"
                     /locus_tag="ACHKJF_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP0951171.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(6003..6881)
                     /gene="nadK"
                     /locus_tag="ACHKJF_003902"
     CDS             complement(6003..6881)
                     /gene="nadK"
                     /locus_tag="ACHKJF_003902"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP0951172.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            7004..7597
                     /gene="grpE"
                     /locus_tag="ACHKJF_003903"
     CDS             7004..7597
                     /gene="grpE"
                     /locus_tag="ACHKJF_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP0951173.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(7652..8938)
                     /gene="yfjD"
                     /locus_tag="ACHKJF_003904"
     CDS             complement(7652..8938)
                     /gene="yfjD"
                     /locus_tag="ACHKJF_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP0951174.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(8959..9750)
                     /gene="ypjD"
                     /locus_tag="ACHKJF_003905"
     CDS             complement(8959..9750)
                     /gene="ypjD"
                     /locus_tag="ACHKJF_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP0951175.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            9917..11278
                     /gene="ffh"
                     /locus_tag="ACHKJF_003906"
     CDS             9917..11278
                     /gene="ffh"
                     /locus_tag="ACHKJF_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP0951176.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            11415..11663
                     /gene="rpsP"
                     /locus_tag="ACHKJF_003907"
     CDS             11415..11663
                     /gene="rpsP"
                     /locus_tag="ACHKJF_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP0951177.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            11682..12230
                     /gene="rimM"
                     /locus_tag="ACHKJF_003908"
     CDS             11682..12230
                     /gene="rimM"
                     /locus_tag="ACHKJF_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP0951178.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            12261..13028
                     /gene="trmD"
                     /locus_tag="ACHKJF_003909"
     CDS             12261..13028
                     /gene="trmD"
                     /locus_tag="ACHKJF_003909"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP0951179.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            13070..13417
                     /gene="rplS"
                     /locus_tag="ACHKJF_003910"
     CDS             13070..13417
                     /gene="rplS"
                     /locus_tag="ACHKJF_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP0951180.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13494..13976)
                     /gene="yfiB"
                     /locus_tag="ACHKJF_003911"
     CDS             complement(13494..13976)
                     /gene="yfiB"
                     /locus_tag="ACHKJF_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0951181.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(13992..15218)
                     /gene="dgcN"
                     /locus_tag="ACHKJF_003912"
     CDS             complement(13992..15218)
                     /gene="dgcN"
                     /locus_tag="ACHKJF_003912"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP0951182.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            complement(15208..15726)
                     /gene="yfiR"
                     /locus_tag="ACHKJF_003913"
     CDS             complement(15208..15726)
                     /gene="yfiR"
                     /locus_tag="ACHKJF_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP0951183.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         3934 a         3707 c         4098 g         4074 t
ORIGIN      
        1 aaatttaatg cttcaggatt ttcactgaga aagtgaaagg cataaactaa gtatacagct
       61 aaagtagccg ttccaattgt tggtccaata tcgccttgat catgaggttc aaggtttaca
      121 ttgaacttga agattagcca ttcaaatccc caagtaaaaa aaacaactaa tatcatggct
      181 gcgaataatt tcgcgatgtt atctgctgat ggattcatca gaacagattg tttctgaaaa
      241 aattcacaaa gtgagagtcc gaaaaaaata aaaaaaagtg gtctggaact aaatttttca
      301 aaaactttga atagggtttc cattttcttt accttcagga ttatattagc tattctgata
      361 cttaggctac cagagcattt gatctttgag cttgaacgta atcactccac cattgcatca
      421 aactctgtcg ctctatcaga tattctgcac gattgtatgc tgcgataatt tcatcttttt
      481 tcgagtgggc aagcgctgcc tcaagaactt cagctctgaa tttaccagac tcctctgccg
      541 ctgttcgtgc aatagaacgc ataccgtgag ctacaagctc gcctccgaac cccattcgga
      601 tgatagctgc gttggctgtt tgttcatgca tatgattaag aggcgctttt atgctgggga
      661 aaacccattc tctatgccca cttattgatt tcattaattc aaggatgcgc aaagcttctt
      721 tactcaaagg aactttgtga agctttttca ttttcatgaa atcagcagga atgttccaaa
      781 tgctgttggt tgtatcaata tcagaccacc ttgcgcgaac ggcttcaccc ggacgaaccc
      841 atgtcaacaa ttgccattca attagcatac gtgtttccaa ccggattgac gcattcgtca
      901 aagattccat aaaccttggc aattcgcttg ggggaagggc aggcatattt tgcttttttg
      961 gtttactgaa tctttgacca aggttgtcag ccgggttgaa ctcaataagt tcttcagtag
     1021 ctgcccaccg gaagatttca ttcagacgtg aaatgatacg gcgtagagtt tccaatacgc
     1081 ctcgttgctc aataggatca aggtgttgtt ttaagagctt aggtcggatc tcattgatag
     1141 ggacattacc cagaccggga aagacatttc tctctaagct gcgccagatg tctgctgcat
     1201 ggtcttgtga gatacctgat gtctttacct tctcatctaa ccatttccgc gctacggctt
     1261 ggagagtgtg ctcagtagca ctctttaatg ccttcgcctt atcgttgtta tggatttggg
     1321 gatcaacacc atttgccaga aaggagagat attcatcacg taaggctctg gctcttgcaa
     1381 gggtaaggtg aggatatgtc ccaaggctca ttttggttct tttcttgctc actggtactg
     1441 catacctgaa ataccaattt ttcttgcctc ctttcgccaa aggagcgatt cgtagaatca
     1501 gaccatcacc gtcaaacaag ttgatttctt tatcggctgg cttggtgctt ttgatttcag
     1561 tgtcagtgag cttcttagcg atttttgcca ttttgggacc ctcggttttt ggacccttct
     1621 tagtgggtcc cattcagggt gccataactc gtagttctca gcaattctca ctggacgaca
     1681 atagacgtaa aaaagcccgc agagcttgtg ctgtgtgggc ttagtagact tcattgaact
     1741 tcaaacaact aaaaagtggt ggagctggcg ggagttgaac ccgcgtccga aattcctaca
     1801 tcctcggtac tacatgctta gtcagtcttt acattcgctt gccagctgcg gacggacacg
     1861 ccactaacaa actagcctga ttaagtttta acgcttcaac cccaggcagg gcttccacgc
     1921 gatctctttt gggtttgacc tctcttgatc cccgtcctaa gagcggaggc tagggagaga
     1981 gggctctaag caggttatta agctgctaaa gcgtagtttt cgtcgtttgc gactattttt
     2041 tgcggctttt tacgaggcca accgcccctc ggcatgcacc ttgggtttcg caaatcccgt
     2101 cgaatccaga atcagcccca atgtgtaaag gtaagtatac cagatttatg agcgccatga
     2161 ccagcctcaa tggcgttatc gttaaagatt tagcacccat gtagcctgat ttttattcga
     2221 ttaagcaatg ggatggcaac atttgtgtcg gatgtgatag ccaataagat gttcattcgc
     2281 gccgccggag agggaggcgc ggtgaggaac tggtcaataa ttggagtgca ggtttaacgg
     2341 tgggcgtttt tcatgatacg tgctttatcc acctgccatt cgcgttcttt gatatctgaa
     2401 cgtttatcgt gctgtttttt acctttggcg acgccgattt tcactttgca ccaggcattt
     2461 ttccagtaca gggagagcgc cactacggta tagccttctc gattgacgcg accgtacaat
     2521 gagtccagtt cgcgctggtt gagaagtaac ttgcgggtac gggtaggatc gcacaccaca
     2581 tgcgtggagg ccacggccat tggcgtgatg ttagcgccaa acagaaatgc ctctccgtca
     2641 cgcagaagga cgtagctgtc gctgatattg gcttttcctg cgcgcaggga tttaacctcc
     2701 cagccttgca gggcaagtcc cgcttcgaac tcttcttcga taaagtattc gtgacgggcg
     2761 cgcttgttaa gcgcgatggt cgctgaacca ggtttatgtg cttttttctt cgtcataagc
     2821 gtcgtgaatc atcggtaatc tgaaatcgaa aacaccccat atcaatcctg cgggggacaa
     2881 ggctctatct tagcatgaac ccgatgttac ccagcgccgg gatagcgttt tttttacagc
     2941 aggataaatg atattatttg ttggattttt gttgatggaa attgttatgc ctcagattag
     3001 ccggaccgca ctggtaccct acagcgcgga gcaaatgtat cagttagtga atgacgttca
     3061 gtcttatcct cagtttttgc cgggttgtac cggaagtcgg attctggagt ccactcctgg
     3121 gcagatgact gcggcggtag atgtctctaa ggctgggatc agcaaaacgt ttactacccg
     3181 caaccagttg accagtaacc aaagtattct tatgaatctg gtggatgggc cgttcaagaa
     3241 attgattggt ggatggaagt ttacgccgct gagccaggag gcgtgtcgta tcgagtttca
     3301 tctcgacttt gagtttacca ataagttgat tgaactcgcc tttggtcgcg tgtttaaaga
     3361 gctggcgtct aatatggtcc aggcttttac ggttcgtgcg aaagaggttt acagtgccag
     3421 gtaaaattgc cgttgaggtg gcttatgcgc tacctgagaa gcagtacctg cagcgcgtga
     3481 cgctgcagga gggcgcgacg gttgaagaag ctattcgcgc cagtggcttg ctggaattgc
     3541 gtaccgatat cgatttaact aaaaacaaag tcggcattta cagccgtccg gcaaaactaa
     3601 gcgatagtgt gcatgatggc gatcgggtgg agatttatcg ccctctcatt gccgatccga
     3661 aagagcttcg caggcaacga gcagaaaaat cagcgaataa ataacagaca gaaaaaaggt
     3721 gctcattgag cacctttttt aacgtctttg agagcaactt tattattagt taccactcag
     3781 cgcaggtttg ttatcaatat tggtcaacac accgctactg ttaaaggtca gcgtcagcgt
     3841 ttgctgcgtt acaccttcat gacctggttg ctggcggaag acatagaacc aggtattcgt
     3901 accaaatgga tcggacatca gcggtgtacc caatgcgtac gcaacttgtt gttgcgtcat
     3961 gccaacacgt attttggata cgtcgttagc ggtcagatag ttcccctggt tgatgtcagg
     4021 acggtaaacc actcgctcca gagtggaaca gcctgcggtc aacatcaata gtactgctgc
     4081 tgcagcagtc agcgttttac agcgcatagt gatttgattc cttttcgggc ccgagcagta
     4141 cgtggctcat ctgtaatatg ccgatgataa tagacctttc atcactttaa aaccttttgc
     4201 tttccggtct tacggcgttt tgctgtttac tctgaccgtg aagcaggaaa aaagtttacg
     4261 ctgcaagcag ttctttcgca ttcgccagtg tattacgtgt gacttcactg ccaccaagca
     4321 ggcgcgccag ctcttgtaac cgcgcttttt tattcaggga ttgcatatgc gtttctgtca
     4381 tcgcaccatc ggtttctttg ctgacaaaat agtgttgatg accacatccc gcgacttgtg
     4441 gcaggtgggt aacacacatc acctgagttg attcgccgag ttgacgcagc agtttgccga
     4501 caactgccgc tgttggaccg ctaatcccta catccacttc atcaaaaatc agtgccgggg
     4561 tttccatttt acgcgccgtg atgacctgga ttgccagtgc gatgcgggac aattcaccac
     4621 cggatgcgac tttggcaata ggctgcattg gctgacctgg gttggtggtt acccgaaact
     4681 caatacgatc ggcaccgtca gcgcccaggt gatgctcgtc aaatttaaca tcgatcgtaa
     4741 actgcccatg cggcattgag agcgcatgca tactgtcggt gatcagctgt gcaagttctt
     4801 ctgcatattg ctggcgttgt tggtgtaatg cgcgcgcgat ttccagtgcc tgctgatgat
     4861 gtttcgttac cgccagcgca agcgtttctt gtgagtcggc ctgatcgtcc agttgctgct
     4921 gttcttccag tagcgactgg taatactgtg gcaatgcctc agggctgacg tgatgtttac
     4981 gtgccagcga aatctgtttt gagatgcgct gttcaagttc aaatagtcgg ttgggatcga
     5041 gatccagacg atcgcagtag tggcgcagtt catcgctggc ttcagcaatc tggatggtag
     5101 cttcttccag catatcaagt acgccggaca gtttgctgtc catgccaatc aattcgctca
     5161 ccagttgttt agccgtgtaa agctgacttt gcaggtttgc gtcttcaccg tcggccatta
     5221 atgccaatgc attctggctg gtggtcagca attgaccgct gttcgccaga cgtttgtact
     5281 cttcgtcgat ttgttcaaac tctccgggct gcggattaaa ttcgttaagt tcttttaatt
     5341 ggtattgcag cagttccgca cgggcggcgc gttcctgact taactgttga tgatgcgcga
     5401 ggtcacggca gctttgatgc cacaactgat aacgtgcggt catttcctgc agtagagagg
     5461 tttcattggc atagccatca agcaggaatt tttggtgctc aggtttggtg agtaattgat
     5521 gagcgtgctg accatggatc tgaatcagca actgacccag ttcgcgcagt tgtgacagag
     5581 gaacagctgt accgttgatg aaaccacggg agcgaccatc gctgctgatc actcgacgaa
     5641 gcaaacattc atgcccgtct tcaagctgat tttcttccag ccagcgcaga gccgctggcg
     5701 tatctttcag agaaaaacgg gcgcacaggt cagcgcgagc agcgccggta cgcaccatgt
     5761 cggcttcagc gcgaccaccg agacaaagac cgagggcatc tattgcaata gatttacccg
     5821 cgccggtctc gccagttatt acggtcatgc cgctatgaaa atcaatctca agctcacgaa
     5881 cgatagcaaa gttgctgatg gtcagttgtg ccaacatagt cgttttcctg tatgaaaaac
     5941 cattactgtt attgtgtaca gtataaactg gttttatata cagtaaagag gctggcgtaa
     6001 aattagaata atttttttga ccagccgagc ttggtgctta atgtgttgaa ataactgtaa
     6061 tctttcggat gaatcagatt cagatggtaa tcacagcgac gaatcaggac atcttcacct
     6121 tcctgaatcg gcagtgctat ctggctgtcg caactgattt ccaggtcgtt acggcgatgc
     6181 gaaaaacgca gacggatcgt gctgctgctg tttatgacca gtggtcgtgc tgacaacgta
     6241 tgcgggaaca tgggcaccag ggtaatcgca tccagagagg gggtcagaat aggaccgcct
     6301 gcagagaggg aataggcggt ggagcctgtt ggcgtcgaaa taattagtcc atcagatcgc
     6361 tgagaaaacg caaagatctc gtcgatatac acttcgaact caatcatatg cgccactttg
     6421 cctggatgaa gcaccacttc atttatcgcg gtgctgatgc gtttctggca atcttgctga
     6481 cagacttgcg cttccagcaa aaaacgtttc tcgctgatgt agtggccttc cagcacatcg
     6541 gctaactgtt gctgggcgtt atcggggtca aggtcagtca ggaaacccag gttgccacgg
     6601 ttgattccaa taactttaat atcgtaacgg gcgagtgtgc gtgccgcgcc cagcatatta
     6661 ccgtcgccac caacgactac cgcgagatca gctagttgcc caatctccgc gagcgtgcca
     6721 gttttcacat tcttcagttg cagttcgtga gcgatttgtt gctcaacgat gacctcgtaa
     6781 ccttttgtgc acagccagcg gtagagcatt tcatgtgttg tcagtgcagt ggggtgccgt
     6841 gggtgtccca caatgccaat acacttgaaa tgattattca tttttccgag gtccttgttg
     6901 cgaagattga tgacaatgtg agtgcttccc ttgaaaccct gaaactgatc cccataataa
     6961 gcgaagttag cgagatgaat gcgaaaaaaa cgcggagaaa ttcatgagta gtaaagaaca
     7021 gaaaacgcct gaggggcaag ccccggaaga aattatcatg gatcagcacg aagagattga
     7081 ggcagttgag ccagaagctt ctgctgagca ggtggatccg cgcgatgaaa aaattgcgaa
     7141 tctcgaagct cagctggctg aagcccagac ccgtgaacgt gacggcattt tgcgtgtaaa
     7201 agccgaaatg gaaaacctgc gtcgacgtac tgaactggat attgaaaaag cccacaaatt
     7261 cgcgctggag aaattcatca acgaattgct gccggtgatt gatagcctgg atcgtgcgct
     7321 ggaagtggct gataaagcta acccggatat gtctgcgatg gttgaaggca ttgagctgac
     7381 gctgaagtcg atgctggatg ttgtgcgtaa gtttggtgtt gaagtgatcg ccgaaactaa
     7441 cgtgccgctg gacccgaatg tgcatcaggc tatcgcaatg gtggaatctg atgacgttgc
     7501 gccaggtaac gtactgggca ttatgcagaa gggttatacg ctgaatggtc gtacgattcg
     7561 tgcggcgatg gtaactgtag cgaaagcaaa agcgtaattt ctgttttcgt aataattcac
     7621 ggccctgcat gcgaatgcag ggccgttttc gttactccgc cacactctcg cgcagcggtt
     7681 tcacaggaaa aacttttacc tgcttaatca tattgtcctg tacgtcgaga atatctatat
     7741 cgtactcgcc aatacgcacg cgggtgcctg cgacagggat ctcttccagt gcctcaagaa
     7801 tgacgccatt aaccgtgcgg gcatcatctt ccggtagatg ccagttaaag gctttgttga
     7861 tttcccgcac gttggcggtg ccatcgataa tcaccgaacc gtcgttttgc ggcgtgacct
     7921 cttcggcaag tgttggcgac atcgacgtag tgaaatcgcc gacaatctct tccagaatat
     7981 cttcaaccgt caccagcccc tgaatgtcgc catactcgtt gacgaccagg ccgactttct
     8041 ttttgttgcg ctgaaacttt accaactgcg tgctgagcgg cgtaccttcc ggcacaaaat
     8101 agatctcgtc cgcggcgcgc agcatggttt ctttggtgaa ctcttttttc tccgacatca
     8161 accgccaggc ttcacgtact cgcagcatac tgatggcgtc gtccagcgaa tcacggtaga
     8221 gcacgatgcg cccgtgaggt gagtgggaga gttggcgcag aatcgatttc cagtcatcgt
     8281 tgatatcaat accgataatt tcactgcgcg gcaccatgat gtcatcaacg gtcatttttt
     8341 ccagatcgag caccgacagc agcatatcct gattgcgacg ggaaatttgt gagcgtgatt
     8401 cgtgcacgat agtgcgcaac tcttctttgc tcaaagagcc gctaaccacg atatcggttt
     8461 tgatacccat catgcgcatc agcatacggg tgatagcatt cagcaaccag accagcggca
     8521 tcatcaaaat ttgcagcgga gccagcagaa aactactcgg gtaagcgact ttttccgggt
     8581 acagcgcggc aatggttttc ggcaatacct cagcaaatac cagtacgaca aaagtcagca
     8641 ctccagtcgc aattgccacg cccgcatcgc cgtacaaacg catcccaaca atagtgccga
     8701 gcgcggaggc aagaatattg accaggttat tgccgattaa caccaggctt atcaggcggt
     8761 ctggcttacg cagcaatttt tcgacgcgtt tggccgagcg attaccctgt ttcgccatat
     8821 gtcgcagacg atagcggttg agggtcatca ttccggtttc ggacccggaa aaataggctg
     8881 aaatgaccac catgatgatc agaataatga tcaacgtagt agtagaaatg tgttccaggg
     8941 gaaactcctt ttctgggttt agctgattaa ctgctggaca attcggctgc cgaagtaggc
     9001 cagtgtcaga attaccgcgc ccgcaacgtt aaaccagacg acgcggcgtc cacgccatcc
     9061 ttcatggtaa tgtccccaca gcagcacaat atagacaaac cacgccacga tagagagcac
     9121 agccttgtcg atattttcca tgctaaatag gttgtgcatg tagaacaggc cagtgcaaag
     9181 cgtgagcgtt agcagcacca cgccaatctg cgtgatgtgg aacattttac gctcgatact
     9241 catcaatggc ggcatttcct ggttaaatgc cagcttcttg ttcttcagtt ggtaatcaat
     9301 ccacgccagt tgcagcgcgt acagggcggc gataattagc gtggcatagg aaaagagcga
     9361 taagccaatg tgcaccagca tcccaggcgt tgcttccaga tgggtgatgt attcattggg
     9421 catgaaggtc gccagcgcca ggttgataag cgcaaaggca tagacaatgg gtagcagcaa
     9481 ccagccacga ttgcgagaag ccacaatggt cattaccgta cagatcatca aactgaccaa
     9541 tgaaccaacg ttcagcaggc tgaggttttg tccgctatca ccgtcgggca ggatgcgggc
     9601 ttccagagcg attgcgtggc agaccagcgc aatgaccgca gaaataatag ccatgcgccg
     9661 ccagccgccg tttttttgca gcagaccggg aacaatcagc gcaagactga cggagtaggc
     9721 gacaagcgcg agcagagcaa aaacgggcat agtgatgtcg acagtttgag caataagaaa
     9781 gagaagcagt atagcgtcag gtgaacgctg ctccaaccgt tgcataacaa caaagacgcc
     9841 ttcatgttat actgcggcaa aatactgatg atgtgtcgcg attgcggcca accgtttcca
     9901 ccccaggcga gagacaatgt ttgataattt aaccgatcgt ttgtcgcgca cgctgcgcaa
     9961 tatcagtggc cgtggacgcc tcactgaaga caacgtaaaa gatacgctgc gcgaagtgcg
    10021 catggcgctg ctggaggcgg acgtcgctct gccggtagtg cgtgagttta tcaatcgcgt
    10081 aaaagagaaa gcggttggtc atgaagttaa taagagcctg acgccggggc aggagttcgt
    10141 caaaatcgtc cgtaacgaac tggttgcggc gatgggcgaa gagaaccaga ccctgaacct
    10201 ggcggcgcaa ccgcctgcgg tcgtgctgat ggcgggcctg caaggtgccg gtaaaacaac
    10261 cagcgttggt aaactcggta agttcctgcg cgagaagcac aagaagaaag tgctggtggt
    10321 ttctgccgac gtttatcgcc cggcggcaat caaacagctt gagacgctgg cagagcaggt
    10381 gggcgttgat ttcttccctt ctgatgttgg tcagaagccg gtagatatcg ttaacgcggc
    10441 gctgaaagaa gccaaactga aattctacga cgtgctgctg gtggataccg ctggtcgtct
    10501 gcacgttgac gaagcgatga tggacgagat caaacaagtc catgcgtcga ttaacccggt
    10561 tgaaaccctg tttgtggttg acgccatgac cggtcaggat gcggccaata cggcaaaagc
    10621 attcaatgaa gcgttaccgc ttaccggcgt agtgttgacc aaagtggacg gcgatgcccg
    10681 cggcggtgcg gcgctctcta ttcgtcacat cactggcaaa ccgatcaagt ttctcggtgt
    10741 tggcgagaaa actgaggcgc tggagccgtt ccatccggac cgcatcgcgt cgcgtattct
    10801 cggcatgggc gacgtactgt cgctgatcga agatatcgaa agcaaagttg accgcgcgca
    10861 ggcagagaaa ttagccagca agctgaaaaa aggcgacggc ttcgatctca acgattttct
    10921 tgagcaactg cgccagatga aaaatatggg cggcatggct agcctgatgg gcaagctgcc
    10981 gggcatgggg cagatcccgg ataatgttaa gtcgcagatg gacgataaag tgctggtgcg
    11041 tatggaagcc atcatcaact cgatgacgat gaaagagcgc gctaagccag aaatcatcaa
    11101 aggttcgcgt aagcgtcgta tcgctgccgg ttgcggtatg caggtgcagg acgtgaaccg
    11161 ccttctgaag cagttcgacg acatgcagcg catgatgaag aaaatgaaga aaggcggaat
    11221 ggcgaagatg atgagaagca tgaaggggat gatgccgccg ggatttcctg gtcgctaaat
    11281 gaccttatgc gacagagtta ttaactgctg attgcatttt ctccagaaat cagtaaaatt
    11341 ttcgggcttt taatatgaca ccggactccg ttcctcgatg gggtccggtt gttttattca
    11401 cacaagagga tgttatggta actattcgtt tagcacgtca cggcgctaaa aagcgtccgt
    11461 tctaccaggt tgttgtcgct gacagccgta atgcacgcaa cggtcgcttc atcgagcgcg
    11521 ttggtttctt caacccaatc gctagcgaaa aagaagaagg cactcgcctg gatctggatc
    11581 gcatcgctca ctgggttggc cagggcgcaa ctatttctga tcgcgttgct gcgctgatca
    11641 aagaagtaaa caaagcagct taatctgtca cggtggtcat gatgagcaaa caactcaccg
    11701 cgcaagcacc tgttgatccc atcgttttgg gaaaaatggg ttcgtcttac ggtattcgtg
    11761 ggtggctcag agtgttttct tccaccgaag acgccgaaag catttttgac tatcagccct
    11821 ggtttatcca gaaggcgggt cagtggcagc aagtccagct ggaaagctgg aagcaccaca
    11881 atcaggacat gatcatcaag ctgaaaggcg ttgacgatcg tgatgcggcg aacctgctga
    11941 cgaattgtga aattgtcgtg gattcatcgc agctgcctca gcttgaagag ggtgactact
    12001 actggaaaga cctgatgggc tgccaggtag taaccactga aggctacgat ctcggtaaag
    12061 tcgtcgatat gatggaaacc ggatctaatg acgttctcgt cattaaggca aacctgaaag
    12121 atgcgtttgg tatcaaggaa cgtctcgtac cgttcctcga tgggcaggtt atcaagaaag
    12181 tcgatctcac tactcgttca atcgaagtag attgggatcc tggtttttaa accaccggat
    12241 aaacggtaaa agacggcgct atgtggattg gcataattag cctgtttcct gaaatgttcc
    12301 gcgcaattac cgattacggg gtaactggcc gggcagttaa aaatggcctg ctgagcatcc
    12361 agagttggag tcctcgcgac ttcacgcatg accggcaccg taccgtggac gatcgtcctt
    12421 acggcggcgg accggggatg ttaatgatgg tgcaaccctt gcgggacgcc attcatgcag
    12481 caaaagccgc ggcgggggaa ggcgcaaagg tgatttatct gtcaccacag ggacgcaagc
    12541 ttgatcaagc gggcgtcagc gaactggcaa cgaatcaaaa attgattctg gtgtgcggtc
    12601 gctacgaagg tatagatgag cgcgtgatcc aaaccgaaat tgacgaagaa tggtcaatcg
    12661 gcgattacgt tctcagtggt ggtgagttac cagcaatgac gctgattgac tccgtttccc
    12721 ggtttattcc gggagtactg ggacatgaag cctcggcaac ggaagattcc tttgctgaag
    12781 gattgctgga ttgcccgcac tatacgcggc ctgaggtgtt agaagggatg gaagttccgc
    12841 cagtgttact gtcgggcaac catgccgaga tacgtcgctg gcgtttgaaa cagtcgctgg
    12901 gccgtacctg gcttagaaga cctgaacttc tggaaaacct ggctctgact gaagagcaag
    12961 caaggttgct ggcggagttc aaaacggaac acgcacaaca gcaacataaa catgatggga
    13021 tggcgtaagc ccccgagata tcagtttacc caggataaga gattaaatta tgagcaacat
    13081 tattaagcaa cttgaacaag agcagatgaa gcaggacgta ccttccttcc gtccgggtga
    13141 taccgtggaa gtgaaagtat gggttgttga aggttccaaa aaacgtctgc aggcattcga
    13201 gggcgtggtt atcgctattc gtaaccgcgg tctgcactct gcattcactg ttcgtaaaat
    13261 ttccaacggc gaaggcgttg agcgtgtctt ccagactcac tctccggtag ttgacagcat
    13321 ttctgtcaaa cgtcgtggtg ctgttcgtaa agctaaactg tactacctgc gtgagcgtac
    13381 tggtaaggct gctcgtatca aagagcgtct taactaagat ttcgcttaag cgacatcctg
    13441 ttaagaaggg ctggccaatt ggctggccct ttttttgtct gtttgctcgt gttttaaggg
    13501 gttgtaatca ccactgcgac gcggcggttc tcggcgcggc cctgggcggt cttgttactg
    13561 gctatgggat atttttttcc taaaccctgt gtggtgagat tgctgcgtgg aatttgtcca
    13621 cccatagccc atgcatcggc cacgacattc gcccgtttca atgataagcc ttcgttgtaa
    13681 ctgtcttcac catagttatc ggtgtgtcca tccatacggg catgtgttag ccctgtcgag
    13741 gccaatttgg ctgccatggt ttggatctgt tgctggcttt ccgggagcaa tttgtagtca
    13801 ttttttgcga acagaatggc atctgataag cccagcgacc agtcgccggc ggattcagta
    13861 aatccataag attgcatagc ggcgacttgc tcaggagtaa actttccctg aggggactgg
    13921 cagccagtca gtattagtga ggtgaaaatc aggggtgcta ccaggtgctt tatcatatcg
    13981 atatatcctt gttatctcac cagcttttcg gcacgctggt gtttggcctg atacatattg
    14041 tgatcggcaa gctcttgtaa tttttccgca gaggcgtgct caatagtcat cgcgtaacca
    14101 atgcttaatg tcatggtggt ctgatggcca ttatgaagat caaacgggag attaaagatt
    14161 tgtgtcagtg ctgagcatat ctgctgcact tcagattccg actgtacatc atagagcacc
    14221 atagcgaatt catcgccgcc caggcggtat gctttatgtc gcagcccgcc aaattcagct
    14281 aaccgttttg cgatttcaat caagactcta tcgcccgtcg catgacccca ggtatcattg
    14341 atatatttga aattatcgcc atcaagaaat agtaacgccg acgtttttcg ggcatcggaa
    14401 ttgttcatca acgtattgat gccgctacga aacgctgcgc ggttagccag cccggttaat
    14461 gggtcatgta gtgcggtacg cagaagctgc gcatttttgg cctgtaaacg aagctgccac
    14521 tcttccattt catccagcag actgttgaag tcgagagcga agcggtgaaa ctcagcaata
    14581 cgttcttccg aaactcgtcg ggaaaaattg cggttggaac gcacatcatg tacgacatcg
    14641 gtgatatttt tcagtgcttc caccaggcca ttgtgcaaat ggcgggtgag ggtaattgcg
    14701 atgcctgatg ccagcagaat acaaccggtc agtacggcga gcgaaaacca gataaaatgg
    14761 ctgattgaac tgtcgcgagc ggttaagcgt acttcgccaa tggtttcacc attgtgacga
    14821 atcggctgaa tgatgggggc agggaagagc cagtggctta tgaaattgct gaaagtatcg
    14881 cctggatcct tacgggtgta atgccaggat gccagaatat tttgctgctt atcacgtact
    14941 tctgcagttg aaaattgccc ttgttggccc agcgctgcca gtgtttcagt tgctgcaggg
    15001 ccatcggcaa aaacgacagc tgcttccaga ctgtaagtca ttgttgctgc tgtcagtgcc
    15061 aggttttttt gcgcgtactg tttcagggtc agcaccgagg tcacggaaag cagcaaccag
    15121 atcagcatca tagtgataaa tatgctggtc atactgatgt tgcgtaatgc tcttttaaac
    15181 gtggggcgct tattaagaga attatcgtta tccatcattt ttcttccgtg cgagcattaa
    15241 gacatccggg ttgaccttta caccgctacg cgataaggca tccaggttta cggcaaatct
    15301 gacgtcatta ttgtggatga tcagacaaaa ggcgctacca ataatgcact cggtattttg
    15361 ttcggcaatt aataataacg cctttgacgg atattgttcg gttaattcca tctggaatgt
    15421 cggtgactcg ttgccaaaat aaaaaccatt gcagcctgaa atcatcgcct cttgttgagt
    15481 gtgaatgatg acaggcagat aaggcaaaga tgtcgcggca ttctcttgta gggcggtact
    15541 aaaacgtgaa gatgaaaaga tacataactt tggtggccct gatagcgcag gccagcgggt
    15601 gtaactgaca ataccggaaa ccatcatacg cacgtttttc gcaacgtcag aaggctcctg
    15661 agcgagtatt ggtgctcccg tcagcaggag aatcagaagt aagaacagtc ggtgagaaaa
    15721 acgcattcag ggatccccac catcttcgct cgaaatgcat cgatattttt tcagaatgct
    15781 cattaaaata tcattttttc atgcgaccgt cat
//
LOCUS       DAZGXZ010000116        15809 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000116 DAZGXZ010000000
VERSION     DAZGXZ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15809
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.116"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(230..709)
                     /locus_tag="ACHKJF_003914"
     CDS             complement(230..709)
                     /locus_tag="ACHKJF_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951184.1"
                     /translation="MQKYKQESLSAAMKESGLLIAKICACVIAGVVVIWGLVTGTETL
                     LTALKTMHEQYDNSMCYIVCATGWFFLVLSNWLWVTYSLYKARTGSTVGGGLQFMFLL
                     SLLAECFSFFVIFWSLSHLDITDIQLLVMGITTLMLFLSLNVKVVVIINHVASGNSD"
     gene            complement(821..2065)
                     /locus_tag="ACHKJF_003915"
     CDS             complement(821..2065)
                     /locus_tag="ACHKJF_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFP0951185.1"
                     /translation="MTLPVDSLIAYTLEKMQERIVQKGRQQDGKIQSGLLFTGNVHDA
                     IPRRLLLDTRLSPLDKMAWMMIRLYAQNNEGAVFPTYDELQLQLASPGKGKASRETVS
                     RVLLMLRITGWLSLCKRVRDEKGRVRGNIYAQHDEPVTCRDAEILDPRYLDMVADACM
                     SRNRTISQTARDILCEIKNDPGMRHYHSHISQLEARIGSAQTPGQMAARQRVTEGRMQ
                     PGSETEPGVETQSSVLKMLSSDSELSEKQGQKILSSDSEPSEKSEGYHRVRKPNHYVR
                     NITHSVIKNTYVLPEALTGQIREEEAIMLTSQLQALPEELAQGVLESLSGMLGSHSLQ
                     NPVGWLLAVLKRAREGNFYPPKQKQRVVVPASVLAGERGRATAQSVSSGKRTEPISAE
                     RLSGIVAGLRASLRRCSAMRSE"
     gene            complement(2083..2550)
                     /locus_tag="ACHKJF_003916"
     CDS             complement(2083..2550)
                     /locus_tag="ACHKJF_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951186.1"
                     /translation="MAIAERTGISSRGDTYEIWLGANCYHYRSDGYAVLLKEGRNRAY
                     PCRVTSETIKSALAAIVELQQKLTAAEKKKECENHGVLLPAEMLIRCLEDQTGGRWIR
                     AHEPVMLMKRLISNLKKARPECTVPDEALAYLRRNCLVNMGDIPVTSRRMSDV"
     gene            complement(2554..2973)
                     /locus_tag="ACHKJF_003917"
     CDS             complement(2554..2973)
                     /locus_tag="ACHKJF_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XF1762 family protein"
                     /protein_id="HFP0951187.1"
                     /translation="MAEINFQVLRENDAGELTGVATAGRPVARHFDDGLTLEVNRTCT
                     TGERNANSALYGAVWRAAKDMGYRRCITYTRADESGASLRAAGFVRVKELPSGKSWAE
                     SSVALKSTRDLVGSGGVPCVLREIRRMSTTVIHIKGE"
     gene            complement(2988..3596)
                     /locus_tag="ACHKJF_003918"
     CDS             complement(2988..3596)
                     /locus_tag="ACHKJF_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFP0951188.1"
                     /translation="MNNSLSQATNGLLMQLVMDLKSGYLRRCESLGLAREEMQMLQGL
                     TIEEIHYLSNSEVSVIRLDINHENLLRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRIAGIDVRPGRGITLSDEESAELWRLWQKSGITDVESADGLDVMML
                     AAEQMNIPLTAVWHAVRGWHQGTAGFNDVSASRRKLNREKAE"
     gene            complement(3593..4318)
                     /locus_tag="ACHKJF_003919"
     CDS             complement(3593..4318)
                     /locus_tag="ACHKJF_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFP0951189.1"
                     /translation="MSDMSHQERLIRRVRKLLELSRNNSNAHEAGLALARAQQLMAKH
                     GITEQDAGLSSVQTSSSQGAPSDAEKVPAWMFRLVMVVTQTFGCRAYYDWRAVPGGRY
                     RRCVTFYGFSERPQVAAYAFDVLCRQLREATDAYLRTQSRRLKLSTRRARAAQFRDGW
                     VQGIRQVVMPFSVTEEESRLMRLWMEQQDMVTVDSREMKACRGGDLARWQGYEAGKNA
                     SLHHGVGESARQVVPGLMIREAS"
     gene            complement(4311..6044)
                     /locus_tag="ACHKJF_003920"
     CDS             complement(4311..6044)
                     /locus_tag="ACHKJF_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP0951190.1"
                     /translation="MAHKPLDLGAAIMQPGRQTTGAGNVVAMGETPMVLTLDQLRPNP
                     DNPRTTRNPRYDDIKNSIHARGLDTVPKVTRMPDSEPDVYIFSDGGNTRYQILTELWK
                     ETGEPRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGVHKARQIHEEQLG
                     RTVSLRELADLLTHEGLPIHNSTISRMEDALKYLYPWIPDLMESGLGRPQVTALLALR
                     QDAERVWGQFAVAADTDAEFDRVFGESCRKFNSPELWSLEMFRDELIGDLLQALPHPS
                     LDYDRWLLELDPKERNRRHHFGEPEPVVVPPSVRGSSTVQPGAPVSGIRSEVPECQPD
                     TDTEAMPESTVSIFPGTEREQHGITRPLKAVSDTDTERTEKCSRPSQEESAGRGDSGA
                     SSAENALRPEIQPDMYGGKPVFSGDVMEDDAAGAVLASVMTGDTAPGTTDALPASVTH
                     FAVRRDDIEHLQNEAFRLAWELAESAGCAEEIAMDRESDLSAGFGAAVEKCSPVVAFL
                     AGLAGDAPRTVLPVSLKDLLTGGSAPEAWPLLDDDHAVKLLRLLSVLRRLRALQRSVL
                     PDDDEGDENDE"
     gene            complement(6044..7411)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKJF_003921"
     CDS             complement(6044..7411)
                     /gene="dnaB-PI"
                     /locus_tag="ACHKJF_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPI-7-type island replicative DNA helicase"
                     /protein_id="HFP0951191.1"
                     /translation="MAEMSVLCAYEAEQAVLGGLMLENERWDEVVLMLSPDDFYVAQH
                     RAIFRAMSELAVNGQPLDLITLSEHLEHQGRLDVLGGFAYLAELSKNTPSAANIMAYA
                     GIVVEKSVLRQLQATGNTLIADVAAPNATSRDVLDAAERRLFALAQNRTLRERTEASL
                     SSALDTVLQQLESVLGSGGVTGTPTGFDCLDEMTCGLQPGDLVLLAARPSMGKTSLAL
                     SMCLSAALRRTEESVLVFSIEMPKEQLILRMLSMLGRVELNHLRSGNMDDEDWLRVSS
                     AVGMALGDGGMGSLGERIIIDDCCEQTPSSLRAGARRYTRLYGKPSLIMVDYLQLIRA
                     PELENRTQEIAEISRSLKALGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALE
                     QDADLIMFIYRDEVYNPDSLAKGEAEIIVSKQRQGPTGTVRVRFDGRYTLFTPSENDG
                     PGGYA"
     gene            complement(7759..8661)
                     /locus_tag="ACHKJF_003922"
     CDS             complement(7759..8661)
                     /locus_tag="ACHKJF_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951192.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGLPASGRP
                     VIAVHGGYVSYSSPRTGHVFQDGSRPETVMTVSNRERMCEWFSQVWLPLHLAALEGDE
                     NAFFSAEVNMVRRELQSVPGNSVNCMCWASLAALAFDAEERVERQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNASGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCTGRIQAGRHHSGSHPDVSVYTERCAQ
                     GMSLSPVHSARQCPVQPTDSAETE"
     gene            complement(8897..9106)
                     /locus_tag="ACHKJF_003923"
     CDS             complement(8897..9106)
                     /locus_tag="ACHKJF_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951193.1"
                     /translation="MCVGCGYSGVRDGAGKNALVWVKVNPESGLGLCSGCDSFLLQER
                     QRRQRHDQLFSCGLSCKQRKTEGAV"
     gene            complement(9439..10326)
                     /locus_tag="ACHKJF_003924"
     CDS             complement(9439..10326)
                     /locus_tag="ACHKJF_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP0951194.1"
                     /translation="MKILPVISTKGGEGKSTQSANLAGFLADAGLRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLSHPDNIISRTAVRGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFQTYDVIVVDSQGARSVMLEMIVLSATDSVVGMVNPVLPDVR
                     EFIRGTVNVMENLMPYRELGIPLPKVRTLVNCMDYTALARQTLAELTGIINAGRYSKV
                     LPEGAVTLLSTQIYDLNIYKQGHAAGQPVHRLEKVSARCSDSALVTIHSLACELLPEW
                     RAFFDALLENGGKSLWAEN"
     gene            10642..11826
                     /locus_tag="ACHKJF_003925"
     CDS             10642..11826
                     /locus_tag="ACHKJF_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0951195.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTKKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQVTDILQQLKTFSGQYRLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYIDGRREMMQWYADYIDSLSEQ
                     VS"
     gene            12155..13198
                     /locus_tag="ACHKJF_003926"
     CDS             12155..13198
                     /locus_tag="ACHKJF_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0951196.1"
                     /translation="MNDRLRFEVNDNQGCFVFPDAWFGSLLDEFDELIDAYDTDEISE
                     TSYINKLRRLARQEPDFIDVHAYLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAALLTDKILAYNPEDNQGARWLLGPEL
                     LRTGDHEQARHVLKAHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            13341..13570
                     /locus_tag="ACHKJF_003927"
                     /pseudo
     CDS             13341..13570
                     /locus_tag="ACHKJF_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13557..15059)
                     /locus_tag="ACHKJF_003928"
     CDS             complement(13557..15059)
                     /locus_tag="ACHKJF_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0951197.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYEQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTDIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
     gene            complement(15221..>15809)
                     /locus_tag="ACHKJF_003929"
     CDS             complement(15221..>15809)
                     /locus_tag="ACHKJF_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP0951198.1"
                     /translation="IHPLSGVSRGRFTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGA
                     ADSRYTLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARI
                     HHKATYTDTSWNKSGEVSDFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLD
                     AGDASVSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
BASE COUNT         3922 a         4462 c         3652 g         3773 t
ORIGIN      
        1 cactgttcac tttttaacct cttctgccac tggcagcact gttcgctttt taacctcttc
       61 tgccactggc agcgctgctc attttttaat ctctgctgcc actggcagca ctgctcattt
      121 tttaatctct gctgccactg gcaacaccgc tcatttttta atcaccgtta ccactggcag
      181 cactgctcat tttttaacct ctgttgccac tggcaacacc gctcattttt taatcactgt
      241 tgccactggc aacatggtta atgatgacta ctaccttaac gttcagagac aaaaacagca
      301 ttaatgtcgt tatccccatc acgagcaact gaatgtcggt tatatccaga tgagacaatg
      361 accagaaaat cacaaaaaat gaaaagcatt ctgccagcag actcaggagg aacataaatt
      421 gcaggccccc tccgactgta ctgcctgtac gcgctttata aagactgtat gtgacccaca
      481 gccagttact taacaccagg aaaaaccacc ctgtggcaca tacgatataa cacatactgt
      541 tatcgtactg ttcgtgcatt gttttcagcg cagtgagcag ggtctctgtg cctgtcacca
      601 gcccccagat aaccaccact ccggcaatca cgcaggcaca aattttagcg atcaacaggc
      661 cactttcttt catggctgca ctcagacttt cctgtttgta tttctgcatg ctatttacca
      721 ccttgtttca cctgaccagt tcccgattct gttttctttt taaccagtcg ctggcggcac
      781 tggttgattt ttgttcccgc gaacatagcc cgtattgcaa tcactcagat ctcatcgcag
      841 aacatctgcg cagcgaggcc cgtagtccag ccacaatgcc actcagacgt tctgctgaaa
      901 tcggttcagt acgtttcccc gatgaaactg attgcgcagt agctcgcccc ctttctcctg
      961 ccagtacgga ggctggcaca acaacacgtt gtttctgctt cggcggataa aaatttccct
     1021 cccgggctcg cttcagcact gcaagcaacc agcccacagg attctgcagc gagtggctgc
     1081 ccagcatccc tgacagactt tccagaaccc cctgcgccag ttcttccggc agcgcctgca
     1141 actgactggt cagcataatg gcctcctcct ccctgatttg ccctgtcagc gcttcaggca
     1201 atacgtacgt gtttttaatc acactgtgtg tgatattacg tacgtaatgg ttcggtttcc
     1261 gaactctgtg ataaccttct gatttttctg atggttcgga atccgaactc agtatttttt
     1321 gcccctgttt ttcactgagt tcggaatccg aactcagcat tttcaatacc gaactctgtg
     1381 tttctacccc cggttcggtt tccgaacccg gctgcattct gccctcagtc acccgctgac
     1441 gggccgccat ctgtcccggt gtctgggcac tccctatccg ggcttccagt tgactgatat
     1501 ggctgtgata atgacgcatt cccggatcat tttttatttc gcacagaata tcccgggccg
     1561 tctgacttat ggtccggttc cggctcatac aggcatctgc caccatatcc agataacgtg
     1621 gatccagtat ttcagcatcc cggcacgtca ccggttcatc gtgctgggca taaatattgc
     1681 cccgtacccg ccctttctca tcacgcaccc gtttgcacag actcagccag cccgtgatcc
     1741 gcagcatcag cagcacccgg ctgacagtct ccctggaggc ttttcctttc cccggggaag
     1801 caagctgcag ttgcagttcg tcatatgtgg ggaaaactgc accttcatta ttctgtgcat
     1861 acagccgaat catcatccac gccatcttat ccagcggaga aagtcgcgtg tccagaagca
     1921 gtcgacgcgg aatggcatca tgcacatttc cggtaaacag cagtccgctt tgtatttttc
     1981 cgtcctgttg tcggcctttc tgcactatcc gctcttgcat tttttccagc gtgtacgcaa
     2041 tcaggctgtc tacgggtaat gtcattacac ttttcctttt tgttatacgt cagacatccg
     2101 tctgctggta acgggtatat cgcccatatt caccagacag ttacgccgga gatatgccag
     2161 cgcttcatca ggaacggtac attctggtct ggcttttttc aggttactga ttaatcgttt
     2221 catcagcatt actggttcat gtgcacggat ccaccgtccc cctgtttgat cttccagaca
     2281 gcgaattaac atctcagctg gcaaaagaac cccgtggttc tcacactcct ttttcttctc
     2341 tgccgcagtt aatttctgtt gcaattcaac aattgctgcc agcgcagatt taatcgtttc
     2401 actcgtcacc cgacatggat aagcacgatt cctgccttct ttcagaagca ctgcataacc
     2461 atcagagcgg tagtgataac agtttgctcc caaccagatt tcgtaagtat cgccgcgcga
     2521 ggagataccc gttctctctg caatagccat atctcattcc cctttgatgt gaatgacagt
     2581 agtgctcatt ctcctgattt cccggagcac acaaggaaca ccaccacttc cgaccagatc
     2641 gcgtgtactc ttcagggcga cacttgattc cgcccagctt tttcctgaag gaagttcttt
     2701 cacacgaaca aaaccagctg cccgaagaga ggctccggat tcatcagccc tggtgtatgt
     2761 aatgcaacgg cgatagccca tatcttttgc cgccctccag acagcgccat aaagtgcgct
     2821 gttagcgtta cgttcaccag tagtacaggt acgattgacc tccagtgtca gcccatcgtc
     2881 aaagtggcgg gcaacaggtc tgccagctgt cgccactcct gtcaattcac ccgcatcatt
     2941 ttcccgcagt acctgaaagt taatttcggc catgtcattt gtccttgtca ttctgctttt
     3001 tcccggttca gtttccggcg actggccgat acatcattaa accccgcagt tccctgatgc
     3061 catcccctca cggcatgcca taccgccgtc agcgggatat tcatttgctc agctgccagc
     3121 atcatgacat ccagtccgtc cgcactctcc acatccgtga tgccgctctt ctgccataaa
     3181 cgccacagct ctgcactttc ctcatcactc agtgtgatgc cacgtcccgg gcgaacatca
     3241 ataccggcaa tgcgacggcg cgccgccaca tccacactgg aaagtccaaa gtaaaacgcc
     3301 atcagttcaa tggagcctcc cagcgccagt gcccggtcaa tgcgctgcag acgtttctgc
     3361 tccgtccggg cctgctgcag catacgcagc aggttttcat gattaatgtc cagccggata
     3421 accgacactt cagagtttga caggtaatga atttcctcaa tggtcagtcc ctgcagcatc
     3481 tgcatttcct cccgggccag ccccagagat tcgcagcgac gcaggtagcc gctcttgagg
     3541 tccatcacca gttgcatcag cagaccgttg gttgcctgag acaagctgtt attcatgacg
     3601 cctcccgtat catcagaccc ggcaccacct gtcgggcact ctcccccaca ccatgatgca
     3661 gtgaggcatt tttcccggcc tcatacccct gccagcgggc caggtcaccg ccgcgacagg
     3721 ctttcatctc acgggagtcc accgtcacca tatcctgctg ctccatccag agacgcatca
     3781 gccggctttc ctcttctgtc acactgaatg gcatcaccac ctgacgaatc ccctgaaccc
     3841 agccatcacg aaactgagcc gcccgggcac gacgggtgct cagtttcagt ctccggctct
     3901 gtgtacgaag atacgcatcc gttgcttccc ttagctgacg gcacagaaca tcaaacgcat
     3961 aagccgccac ctgaggacgt tcactgaaac cgtaaaacgt cacacaccgg cgataccggc
     4021 ctccgggcac cgcccgccag tcgtaatacg cccggcagcc aaatgtctgt gtcaccacca
     4081 tgaccagacg aaacatccac gccggaactt tttcagcatc agaaggtgcc ccctgtgatg
     4141 aagaggtctg caccgaagaa agtccggcat cctgctccgt gataccgtgt ttcgccatca
     4201 gctgttgcgc ccttgcaagc gccagcccgg cctcatgggc attgctgtta ttacggctca
     4261 gctccagcag tttcctgacc cgcctgatca gacgttcctg atgcgacata tcactcatca
     4321 ttctcatccc cctcatcgtc gtcgggtaat acactgcgct gcagggctcg caggcggcgc
     4381 agcacactca gaagacgcag cagcttcacg gcatggtcgt catccagcag tggccacgct
     4441 tccggtgcac tgccccctgt aagcaggtct ttcagactca cggggaggac tgtccgcggt
     4501 gcatcaccgg ccagacccgc cagaaacgcc actacaggag aacatttctc cactgctgcc
     4561 ccgaaaccgg cagacaggtc gctttccctg tccatggcaa tctcttcagc acaaccggct
     4621 gattcagcca gttcccaggc cagacggaac gcttcattct gcagatgttc gatatcatcc
     4681 cgcctcacag caaagtgcgt caccgacgcg ggaagtgcat cagtggttcc cggggcagta
     4741 tctcctgtca taactgatgc cagtactgcc ccggcagcat catcttccat aacatcacca
     4801 ctgaagacag gcttaccacc gtacatatcc ggctgtatct caggacgcag ggcattttca
     4861 gcagaagacg ccccactgtc ccccctgcct gcgctctctt cctgagaagg tcgactgcat
     4921 ttttcagtgc gctctgtgtc agtatcagaa acggctttca gtggccgggt aataccatgt
     4981 tgctcgcgct cagtaccggg gaatatggag actgtgcttt ccggcatcgc ctctgtatcc
     5041 gtatccggtt gacactccgg tacttcagac cggatcccgg ataccggtgc cccgggttgt
     5101 accgtactgc tgccacggac tgaaggaggc accaccaccg gttccggctc accaaaatga
     5161 tgacgacggt ttcgctcttt cggatccagt tccagcaacc accggtcata gtccagggag
     5221 ggatgtggca gggcctgcaa caaatctccg ataagctcat cgcggaacat ctccagtgac
     5281 cacagttccg gcgaattaaa cttccggcag ctttccccga acacgcggtc aaattccgca
     5341 tccgtatcgg ctgcaacagc aaactgcccc catacacgtt ctgcgtcctg acgaagtgcg
     5401 aggagtgctg taacctgtgg acgccccagt cctgactcca tcaggtcggg gatccagggg
     5461 tacagatact tcagggcatc ttccattctg ctgatcgtgg agttatgtat cggcaaccct
     5521 tcatgggtta acaaatcagc cagttcacga agagacactg ttctgcccag ttgttcctca
     5581 tggatttgtc ttgccttgtg gaccccctgc gccttctcga taaaagtcag ttcaccgcga
     5641 acttcatttt cagccagatg accaatcaca cactggagcc gacccggcca gggtttaaac
     5701 agcacatgaa tacggtaaaa acgcggctcc cctgtttctt tccagagttc cgtcagtatc
     5761 tggtaacggg tgtttccccc gtcactgaag atatacacat ccggctcact gtccggcata
     5821 cgggtgactt tgggcaccgt atccagcccg cgggcatgaa tggaattttt gatatcgtca
     5881 taacgcggat tgcgcgtggt gcgcgggtta tccggattcg gacgtaactg gtccagcgtc
     5941 agaaccatgg gcgtttcccc cattgccacc acattgccgg caccagtggt ctgacgaccg
     6001 ggctgcataa tagccgcccc gagatccagt ggtttatgtg ccatcacgca tatcctcccg
     6061 gcccgtcatt ctcagagggc gtaaacagtg tgtaacgacc gtcgaacctg acccgtaccg
     6121 tacccgtggg cccctgacgc tgcttgctga caatgatttc tgcctccccc tttgccagtg
     6181 agtcggggtt atagacctcg tcgcggtaaa taaacataat caggtcagca tcctgctcca
     6241 gcgccccgga atcacgcagg tcaccgttat tggggcgttt atcagcacgc tgctccacct
     6301 gccggttaag ctgggagagg gccagcaccg ggcagcccag ctccttcccc agcgctttca
     6361 gggaacggga aatttcagca atttcctgtg tgcggttttc cagctccggc gcacgaatca
     6421 gttgcagata atccaccata atcagggagg gtttaccgta cagacgtgta taacgacggg
     6481 cgcctgcacg cagtgatgaa ggcgtctgct cacagcagtc atcaattatt atgcgctcac
     6541 ccagagagcc cattcctccg tcaccaagcg ccataccgac cgcagatgac accctcagcc
     6601 agtcctcatc atccatgttg ccactgcgca aatgattcag ttccacacgc cccagcatgg
     6661 aaagcatgcg cagaatcagt tgctccttcg gcatctcaat gctgaatacc agaacgctct
     6721 cttccgttcg ccgcagtgcg gcgctgagac acatggatag cgccagagag gttttcccca
     6781 ttgacggacg ggcagccagc agaaccaggt caccaggctg caggccacag gtcatttcat
     6841 ccagacagtc aaatcccgtg ggggtaccgg taacaccacc ggagccgagc acactttcca
     6901 gttgctgcag aacggtatca agcgcggatg aaagagaggc ttccgtgcgt tcccggagcg
     6961 tcctgttctg cgcaagtgca aacagacgcc gctctgcagc atccagcaca tcccgtgatg
     7021 tggcgttcgg tgccgccaca tcagcaatca gggtgtttcc ggtggcctgt aactgacgca
     7081 gaacgctttt ctccaccaca atgcctgcat aggccatgat gttggccgca gaaggtgtgt
     7141 tcttgctgag ctcggccaga taagcaaagc cgcccagtac gtccagcctg ccctgatgtt
     7201 ccaggtgctc tgacagtgta atcaggtcca gtggctggcc attcaccgcc agttcagaca
     7261 tggcacggaa aatagcccgg tgctgagcca cataaaaatc gtcaggactc agcatcagca
     7321 ccacttcatc ccagcgttca ttctccagca tcagaccgcc cagaacggcc tgctctgctt
     7381 cgtatgcgca gagaacagac atttcagcca tggcgccccc tccccctctg acagccggaa
     7441 acctggctac gtgaaaaaag gaaaaagggt gccggacgta cagctgtcgg gaataaaatc
     7501 ccggtaactg tctgtacgtt ttcagaccgg tgagtttgcc gcccccggtc tgaaggtacg
     7561 gacagcattc gttcattcat aacaagttgt tttaaaggaa aaagtttatg aatacagaaa
     7621 aacaactgcc gtccggcatt gaatctgaag tgaaatacac cctgcagttc tggctgctga
     7681 gtaacggaaa ggtcatgacg gaaaaagacg ggctctgtaa cccctttccc ctgaactgta
     7741 ttgaatcggc tcatcctttt actccgtttc agcagaatca gtgggttgaa ctggacactg
     7801 tcgggccgaa tggaccggtg aaagcgacat tccttgtgca cagcgttctg tataaactga
     7861 aacgtcaggg tggctccctg aatgatgccg gccggcctga atacggccgg tgcattgctc
     7921 actggcacac tgacgtgttt gttgagaaaa aagcgctgcc tgactggctg agagcattcc
     7981 acactgacgt ttcgcactcg taaaaacacg ttcagcccga cgggtcatat tttccagcca
     8041 cccgtcgggg aaaatgtcag caccatcacg gggaaacaaa atcgctttcc attcagggta
     8101 tgagcgcgga atacctccgg aagcattccg gctcagacag cgactcacag tgcaggcagc
     8161 acggaatgca tatgttgcga tgctctcatc atatgttgcg atgttctcat tattgccttt
     8221 ctgccgttcc acccgctctt cagcatcaaa tgccagtgca gcaaggcttg cccagcacat
     8281 gcagttcact gaattccccg gaacgctctg cagctcccgg cgcaccatgt tgacctcagc
     8341 actgaaaaaa gcgttctcat cgccttcaag ggcagccaga tgcagcggaa gccagacctg
     8401 actgaaccat tcgcacatcc gttcccggtt actgacggtc atcactgtct ccgggcggga
     8461 accgtcctga aaaacatgtc cggtgcgtgg agaactgtat gaaacataac caccgtgcac
     8521 cgcaatgacc ggacgacctg acgccggaag ccccacacga cacccactgg caatccgggt
     8581 catcagcagg ttcagttcag ccagtaattc agggcgggtt aagaccgccg gaaccggaga
     8641 atatttcact gaggaagaca tactttcacc tccatatctc tcagcccgcc aggtgcgtca
     8701 cttccccgca gagaaccgga aacctggcta tatgaaaaaa gggaaaaggg tgccggacga
     8761 acagttgtcg ggaataaaat cccggtaact gtctgtacgt tttcagaccg gtaagtttgg
     8821 cgaccctggt cagaaggtac ggacagcatc tttgcgctca taactgtacc gaccgggata
     8881 tgcattacaa agactgtcat accgccccct ccgtctttcg ctgtttgcag gaaagaccac
     8941 atgaaaaaag ctggtcatgc cgctgacgac gctggcgttc ctgcaaaaga aaggagtcac
     9001 aaccggaaca cagccccaga ccactctcag gattcacctt cacccagacc agagcattct
     9061 taccggcacc atcccgtacc ccggaataac cacagcccac gcacaccgtc cacttcttca
     9121 gtacgatgag tgcagtttcg agtgatgcgc ggagacgccg ggaggcaata aggagcaacg
     9181 gctcatggtc ataaaaaacc ccgccgttac cccctccgga ttcaagcagc gggagtcctg
     9241 caggctcccg gggacctgtt gacatttccc gtaattcgtt ttcattccac atattgtgga
     9301 aagttatccg gttcactgaa caacgccatt cctccgccag acgctgatac aaatgctttt
     9361 tcgcttctgc gcggtcagat gcatcaacaa acacatgcac ttcctcgtgt ccgggtgctg
     9421 aagccacggc acgccatatc agttttctgc ccacaggctt ttccctccgt tttccagcag
     9481 agcatcaaaa aacgcacgcc actccggcaa cagttcacag gccagactgt gtatggtgac
     9541 cagcgcagaa tcgctgcaac gggcagacac tttttccaga cgatgcaccg gctgaccggc
     9601 tgcgtgcccc tgtttataaa tattcaggtc gtaaatctgt gttgaaagaa gggtgaccgc
     9661 accttcaggc agcactttgc tgtagcgacc ggcgttgata atgcctgtca gctctgccag
     9721 tgtctgtctg gccagcgccg tataatccat gcagttaacc agggtgcgga ctttcggcag
     9781 gggaataccc agttcacggt agggcataag gttttccatg acattgaccg tgccgcggat
     9841 gaactcacgc acatccggca gaaccggatt caccatcccc accacagagt ccgtggcgga
     9901 cagcacaatc atttccagca tgacagaacg tgcgccctgt gaatccacca caatgacgtc
     9961 ataggtctga aaaagcggat gctgaagcac attacgcaga cgaaggcgac catccggcgc
    10021 atgaagcatg gccgttttca gttgctcatg ggggtcgttg gagacgatga ggtcaagccc
    10081 cctgacagcc gtacgggaaa tgatgttgtc aggatgactg aggtcagccg tgcgcatcag
    10141 taattcaaaa agaccggccg gcgcttcata cgtcagcgga aagatattgc ttgccgtggg
    10201 ctgtgcatgg tctccgtcta tcagcagagt acggaggccg gcatctgcca gaaaaccggc
    10261 aagattagca gactgggttg attttccttc gccaccttta gtggatataa ccggtaaaat
    10321 tttcatatag cacactcttt aaagtatgca accaatcact tacactacgg aaaccagcca
    10381 gaatattcac ttcactacct gaatactcgc aacacgggga ttgaaaatcc ccgtgtcctt
    10441 ggttcgattc cgagtccggg caccactttc acttcctgat tcatctcccg aactccacga
    10501 aactcaagta taatcacatc gttgcaagca atctgaacat tctgaactcc accagactct
    10561 actgaactct ctctcaatca agcgttcatt gggggccccg gtgggggctc attgtgttca
    10621 gtcagtttca ggagccccca tatggcactt acagccagac aggtcgaaac agccagacct
    10681 aaagaaaaag actataaact ctctgacgaa cgtggtttat atctgctggt aaaaaccacg
    10741 ggagcccgct actggcggct taaataccgg atagcaggaa aagagaaaaa actggccctc
    10801 ggcgtctatc ccgacgtctc ccttgctgag gccagaatca aacgcgacga tgcccgaaaa
    10861 atcatctccg aaggtggtga cccgggcgaa aagaagcgaa aggaaaaact cactaagaaa
    10921 atctctgcca ccaatacgtt ccatgccctc gctacggaat ggcaccagca taaatctttg
    10981 tcatggtctg aaagttacgc cagaagcgta ctggaagcgc tggataaaga tattttcccg
    11041 tatctgggca aacgaagcgt tacggatatc ctcccgctgg aaatgctgga aattctgcgc
    11101 cgcatagaaa aacgtggctc gctggaaaaa ctgcgtaagg tccgtcaata ctgtaatcag
    11161 atttttcgtt atgccatcgc caccggacga gccactgtca atccggcatc tgaactgacc
    11221 agtacactgg cggcgccaaa agcggcgcat ttcccccacc tgagagcaga tgagctccct
    11281 gtttttctca ggaagctcgc tgaatatcat ggcagtccag ttacccgcat ggcgacaaac
    11341 ctgctgcttc tgactggcct cagaaccatt gaactacggt ctgctgaatg gtcagaaatt
    11401 gattttgata atgccctgtg gacaatccct gaaagccgca tgaaaatgcg acgtaaacat
    11461 gtcgtaccac tgtcacgaca ggtcactgac attctgcaac aactcaaaac tttctccgga
    11521 caataccggc tggttttccc gggacgttgt gatatcaaca agccaatgag cgaagccagc
    11581 atcaatatgg tgctcaaacg tatcggttac gatggcagag caaccggtca tggttttcgt
    11641 cacaccatga gtaccattct gcacgaacag gggtttaatt ctgcctggat tgaaatgcag
    11701 ttagctcatg tggataaaaa ttccatcagg ggtacctata atcatgccct gtatattgat
    11761 ggtcgccgtg aaatgatgca gtggtatgct gactacattg attcgctttc tgagcaggta
    11821 agttaagctg agaacacgtt attctgattt tcacttactt cacgattatt cctccggcct
    11881 gcaggttcgg aggattctta tttttatgtt ttattttcag ataaagtttt tgacaaaacg
    11941 tttattctgc atcagcaaaa ttatcacaaa acaacgcaat cacagacaaa ttaacatttt
    12001 tcagacgggt atattgagtg cccacaatgt aagacaggca ggcatccggt gacatggtaa
    12061 aatattaatc cagggcaaaa acaaggcttg atacacaaca tcatggcttt tatgctctca
    12121 gactatgcca ccacagattt tacaggctca gaaaatgaat gacagattac gctttgaagt
    12181 gaacgataac cagggctgtt tcgtgttccc ggacgcctgg tttggctcgt tgctggatga
    12241 gtttgacgaa ctaattgatg catacgacac agatgaaatc agcgaaacaa gttatatcaa
    12301 caaactcaga cgccttgccc gacaggagcc tgactttatt gatgtacacg cttacctggc
    12361 atacgcattt ctggagcaga atgccccgcg taaggcactg aacgctgctc ttaaaggact
    12421 ggcagcaggt aatcgtctca tcccggaaag tttcagtggt gaaatcatct ggatgcatcc
    12481 cgaaaaccgg ccttatctgc gcgcgcttta tgcagcaatt ctcgcaaatg tgcacttaca
    12541 gcgtcatcag gatgcagctc tgctcactga taaaatactg gcatacaatc ctgaagacaa
    12601 tcagggagca cgctggctac tgggaccgga actgttacgt actggcgacc atgaacaggc
    12661 acgccatgta ctgaaagcgc atgcagatga attttcacct tactggtatg aactcggcct
    12721 tctgcatttt cttaatgggg agctggtaaa agctgcaact gcatttcgtc gcggctttgc
    12781 ggcaaatact tacatcgcag aaatactgtg cggaaatctg catccatttc cacttgctgt
    12841 atggcataac ttttcaggcg gtccggatac tgctgaagat tattatgcca cataccaccc
    12901 gttatggggg caatatccgg aagcattgct gtttgtaaac tggctctata atcattcctc
    12961 tgtgctgcat gaacgggcag aaatcattaa atgcgccgaa atgctgatgc aggaagatga
    13021 tttcgaaata tgcgaaagta ttttaagaca gcaggagaag ttgcgtgaaa gaattgatga
    13081 gacgctttct gagaaaattg tacagaaatg cagaaatatg aatggtgaat atgtctggcc
    13141 ctggatattg ccgttttcag cggcaggcat gaaacatact ggcatacagt atcagtagat
    13201 attgcattag tgtatcctgc acacaagtaa taatttatcc accaataata acactgttaa
    13261 tgtccccttc ccctggttgt cagccagggg ttatcttctg aatatttctt ttgaaaagga
    13321 taacacagta aattattttt atgaattatc ccatggactc attaacaccc tttcataatg
    13381 ctttattgtc aaacacgtta tggctgacat caaaaaaacc ggatttcctc tgccagcggg
    13441 taatcacctc cccggtgttt tcggttggtc tggttactcc agtctggtaa ttagcaagat
    13501 aatcgatata aacagtggaa aactcatcgt acataatctg gtgatgaaca ttacgcttat
    13561 tttcccctga ccggaagaat cagaggctgc ggtttcagac tgtctgccgg tacattcctc
    13621 tccccgttaa aaaccataac gggttcatta tcttcgtctg tcagcggatt gaatggcggt
    13681 atattttcag tacgaatgtc ggtcagccac tgaaaaatac ctgcgaaatg acgggcactg
    13741 attttcctgc tgacggactg atgagacgtg atgtcactgg cggtaataat caggggaacg
    13801 ctgtagcctc cctgcacatg accatcatga tgaacaggat tggcactatc gctgaccgac
    13861 agcccatggt cagaaaagta aagcatggca aaatgacggg aatgccggcg aaggatacca
    13921 tcaagctgac cgagaaagtt atccagttta ctgatgctgg cgaggtaaca ggcaatcttt
    13981 cggggatact gctccaggta atgattcggc caggagttaa gtcggtcaca cggattcgga
    14041 tgagagccaa tcatgtgcag gaaaatcacc ttcggagagg atttatccgc cagcgcacgt
    14101 tctgtttcct gtaacaacaa catgtcatcc gttttacggg aagcgaagct gcctttcttg
    14161 aggaaaacgg tatgctccgc atcagaagca ataacagaga tgcgtgtgtc atgctctccc
    14221 agttttccct gattggatat ccaccatgtg ctgtatcctg cttttgctgc cagcgccacc
    14281 acgttgttgc cggaatcagg gttctgctca tagtcataaa tcagtgtccg gctcagggaa
    14341 ggtacggtac tggctgctgc cgatgtatag ccgtcaataa ataaaccggg agccgtattc
    14401 agccacggtg tggttggtac gggataacca tataccgaca tataatccct gcgcacactc
    14461 tcaccagtga caatcacaat cgtgtcatac aacggtgctc ccggcaggat tttccagctg
    14521 tcagccctgt gctgactcag ttgttcataa cgctgcattt cacgcaatgt gtcagttgtc
    14581 cccacaacgg ttcctttaac catccgcaac ggccagctgt ttacagagca taatacgaac
    14641 agcagcagtg ccagccagtt acggtgaccg cggtggtgtg tccgccagaa aataaccatg
    14701 aacaccagaa tcgcggcact gaccagaaaa tgataaacag gaatcatccc ggtaaactcc
    14761 gcagcctcat ccgccgtggt ctgcaacaac gcgacaataa aactgttgtt gattttaccg
    14821 tacgtcatac ccgcaggcgc atacagtgca caacagaaca gaaataacag agctgtaatg
    14881 gatgtaaggg tatttctgtg tgcaaggagc agaagaagga acagcagcaa cacattcccg
    14941 gtggcattct tctcagtgta tccgcatgca attgtggtta tgacagaaat aacaaaaaag
    15001 aataaaaaca ataaaatccg tggtgtgttg tccggacaaa acagttttct gatattcatc
    15061 ggagtatatc ggcaacatta ttatgaagag aataaaataa taagaaaaac agaaatatcc
    15121 tgtaaagcag ataacagaca accctgcagt ataattacgg cagggagttt ccgtttaatt
    15181 acagaagaca ggtaattatt atctttcagg agtagtttcg tcagaagtta taagtcacac
    15241 caagcatgat gtcatgactt ttaacatcaa cttctgattt atatttatcg ccccactcat
    15301 ccttgtaact cacactggca tcacctgcat caagatagcg atagctgagg tccagagcga
    15361 tatccggggt tacgtcatag cggacacctg ccccaaaact ccatgcaaaa ttgttatcat
    15421 atcccgggta atgcaacgca gaaaaatcgc tcacctcacc agatttattc caggaggtat
    15481 cagtatatgt tgccttatga tgtatccgtg cataacccag tccaacagac acccatggag
    15541 tgaatgcact gctgtttctg aagtcataat acgtattcac catcagagta ttcacactga
    15601 gcctgttctg tgtgtcttcc cgaccaccac cgaccatcgg agagtaccat gtatccagtg
    15661 tataacggga gtccgcagct ccgcggccat agaattccac ttccgtgcgc acaggaacat
    15721 tgtaatgctg ataaaaatca tagcccaccg caaggccggc accgaatacc gtgaaccggc
    15781 cccttgaaac gccagacagt ggatgtatc
//
LOCUS       DAZGXZ010000117        15716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000117 DAZGXZ010000000
VERSION     DAZGXZ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.117"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2390
                     /locus_tag="ACHKJF_003930"
     CDS             <1..2390
                     /locus_tag="ACHKJF_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0951199.1"
                     /translation="LIEAEDRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQ
                     WVLIDNQTAVCLTPEQLTLLGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQL
                     SISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAG
                     VNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIF
                     DSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFA
                     ITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDS
                     LQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDA
                     QLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQN
                     EKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSFSLSRDFDIGPLKNVSTSL
                     TFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWS
                     VSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHG
                     AAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRN
                     LPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSH
                     KELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            2406..3155
                     /gene="yqiH"
                     /locus_tag="ACHKJF_003931"
     CDS             2406..3155
                     /gene="yqiH"
                     /locus_tag="ACHKJF_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0951200.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3157..4221
                     /gene="yqiI"
                     /locus_tag="ACHKJF_003932"
     CDS             3157..4221
                     /gene="yqiI"
                     /locus_tag="ACHKJF_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951201.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4264..4464)
                     /gene="glgS"
                     /locus_tag="ACHKJF_003933"
     CDS             complement(4264..4464)
                     /gene="glgS"
                     /locus_tag="ACHKJF_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP0951202.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            4733..5362
                     /gene="yqiJ"
                     /locus_tag="ACHKJF_003934"
     CDS             4733..5362
                     /gene="yqiJ"
                     /locus_tag="ACHKJF_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0951203.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            5389..7050
                     /gene="yqiK"
                     /locus_tag="ACHKJF_003935"
     CDS             5389..7050
                     /gene="yqiK"
                     /locus_tag="ACHKJF_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0951204.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            7289..7348
                     /locus_tag="ACHKJF_003936"
     CDS             7289..7348
                     /locus_tag="ACHKJF_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0951205.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            7665..7724
                     /gene="ibsE"
                     /locus_tag="ACHKJF_003937"
     CDS             7665..7724
                     /gene="ibsE"
                     /locus_tag="ACHKJF_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP0951206.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(7844..9277)
                     /gene="hldE"
                     /locus_tag="ACHKJF_003938"
     CDS             complement(7844..9277)
                     /gene="hldE"
                     /locus_tag="ACHKJF_003938"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP0951207.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(9325..12165)
                     /gene="glnE"
                     /locus_tag="ACHKJF_003939"
     CDS             complement(9325..12165)
                     /gene="glnE"
                     /locus_tag="ACHKJF_003939"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP0951208.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFVQD
                     SVIVHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(12188..13489)
                     /gene="ygiF"
                     /locus_tag="ACHKJF_003940"
     CDS             complement(12188..13489)
                     /gene="ygiF"
                     /locus_tag="ACHKJF_003940"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP0951209.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYHDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            13731..14351
                     /gene="ygiM"
                     /locus_tag="ACHKJF_003941"
     CDS             13731..14351
                     /gene="ygiM"
                     /locus_tag="ACHKJF_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP0951210.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            14415..15653
                     /gene="cca"
                     /locus_tag="ACHKJF_003942"
     CDS             14415..15653
                     /gene="cca"
                     /locus_tag="ACHKJF_003942"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP0951211.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAIASWKEQRCPKPD"
BASE COUNT         4096 a         4007 c         3747 g         3866 t
ORIGIN      
        1 atttaatcga agccgaagat cgtgaaaacg ttaacctttc ccaatttgaa actgatggcc
       61 aattacccgt aggtaaatac tcattaaacg ctcttattaa taataaaagg acgccaatcc
      121 atctggacct gcaatgggta ttaattgata accaaactgc agtttgtctg actccagagc
      181 aattaacatt attaggcttt actgatgaga ttattgaagt ggctcagcaa aacctgatcg
      241 atggttgtta ccctatcgaa aaagaaaaac aaattacaac ttatctcgat aaagggaaaa
      301 tgcaattatc catatctgca cctcaggcat ggttaaaata caaagatgca aactggacgc
      361 ctcctgaact ttgggatcat ggtattgctg gggcatttct tgactacaat ttatatgcct
      421 ctcattatgc accacatcag ggcgataatt cacaaaatat aagttcctat gggcaggctg
      481 gggttaatct tggggcctgg cgcctgcgta ctgattacca gtacgatcaa tcatttaaca
      541 atggcaaaag ccaggcgaac aacctggatt ttccgcgtat ttatttgttt cgcccaatcc
      601 cagcaattaa tgcaaaacta actataggtc aatacgatac tgaatcctct attttcgact
      661 ctttccattt ttctggcgtt tcgttgaaaa gcgatgagaa tatgttgccg cctgatctac
      721 gtggttacgc accgcaaatc acgggtgttg cacaaacgaa tgcaaaggtc actgtctcac
      781 agaacaaccg tattatttat caagaaaatg ttcctcctgg cccatttgct attactaatt
      841 tattcaatac attacagggg caactggacg tcaaggttga agaagaggac gggcaagtca
      901 cgcaatggca agttgcatct aatagtattc cttatctgac gcgtaaaggg cagattcgct
      961 acaccactgc tatgggtaaa ccgaccagcg ttggtggtga ttccttacaa caacccttct
     1021 tctggactgg tgaattctca tggggttggc tgaacaatgt atccctgtat ggtggttcag
     1081 ttttaacaaa ccgtgattat caatctctgg ctgccggcgt tggctttaac cttaactcat
     1141 tgggttcatt atcttttgat gtcacacgat ctgatgctca gttgcataat caggataaag
     1201 aaacgggtta tagctaccgc gctaactatt caaaacgttt tgaatctacc ggtagccagc
     1261 tcactttcgc tggttaccgt ttctctgata aaaactttgt gacaatgaat gaatatatca
     1321 atgacactaa ccattacacg aattatcaga atgaaaaaga gagttatatt gtcacgttta
     1381 accagtatct tgaatcatta aggttaaata catacgtaag tttggctcgt aatacttact
     1441 gggacgccag cagtaatgtg aattattcat tttcacttag ccgcgatttt gatatcgggc
     1501 cattaaaaaa cgtctccact tctctaacat ttagccgaat aaactgggaa gaagacaacc
     1561 aggatcaact gtacctaaat atttcgattc cctggggaac cagtagaaca ttgagctatg
     1621 gtatgcaacg aaatcaggat aacaagattt cgcatactgc ttcgtggtat gactcttccg
     1681 atcgaaataa ttcctggagc gtttctgctt caggcgacaa tgatgaattc aaagatatga
     1741 aagcgtcact acgcgccagt tatcagcata ataccgagaa cggtcgtctc tatctctccg
     1801 ggacatcaca gcgagacagt tattattctc tgaatgccag ttggaatggt tcattcacgg
     1861 cgactcgtca cggagccgct ttccatgact atagtggtag tgctgactcg cgttttatga
     1921 tcgacgcaga tggtgcagaa gatattccgt tgaacaataa acgcgcggta actaatcggt
     1981 atggcatcgg agttattcca tcagtcagca gttacataac aacatcatta agcgttgata
     2041 cccgaaatct tccagaaaat gtggatatcg aaaactcggt tatcaccacc accttaaccg
     2101 aaggtgctat tggctacgcc aaacttgata cccgcaaggg ctatcaaatc atgggggtta
     2161 ttcgcctggc agatggtagt cacccaccac tggggattag cgtaaaagat aaaaccagcc
     2221 ataaagaatt aggactggtt gctgatggcg gctttgtata cctcaacggc attcaggatg
     2281 acagcaaact tactttacgc tggggtgata aatcctgttt tattcaacca cccaatagca
     2341 gcaacttaac caccggaacg gttattttac cgtgtattag ccaaaattaa ttcaggatga
     2401 aactaatgcg ctacttaaac acaaagaatc tcattgcagc aggagtatta ctcgcctgca
     2461 tgagcagcat tgcctggggt gccatcatcc cggaccgtac ccgtatcata atgaatgagt
     2521 cagataaagg ggaagcatta aaacttacta atcaaagcaa aaatctaccc tatctggcgc
     2581 aaacatggat agaagatacg aaaggcaata aatcacgtga ctttattgtc acggttcccc
     2641 ctatggtacg tttaaatccc agtgagcaaa ttcaaataag aatgataagt caggaaaaaa
     2701 ttgctcaact gcctaatgac agagaaacgc tcttctattt taacgtgcga gaaataccgc
     2761 caaaaacaga taaaaaaaat gtgatgcaag tcactatgca gcacgcattg aaactattct
     2821 ggcgaccaaa agctattgaa ttagaagatg atggggtaat gtcttacgaa aaagttgaaa
     2881 tcatccgtag aaatgatggt tcgatccgct ttaacaataa gatgccttat cacgttacgc
     2941 tgggttatat cggaacaaac ggaatcacaa tgttgccaca aacgcaaagt ttaatggtaa
     3001 ccccttatag ccatgcagat acgcagttta aaaatgttcc gtcagctttt caggttggtt
     3061 atatcaatga ttttggtgga ttaagttttt atgaaataaa ctgcccgacg gttaataact
     3121 cttgtaatgt ttccgtagcc aaacgagata aataatatgc gctacttatt aattgtaatt
     3181 atatttatta taggttttag tgcattacct gtatgggcaa tggactgcta tgctgaacat
     3241 gaagggggaa ataccgtagt cattggttac gtaccaagaa tttctatccc cagtaatggt
     3301 aaaaaaggtg ataagatctg gcaaagcagt gagtatttta tgaatgtctt ctgtaataat
     3361 gcattacctg ccccatcacc tggagaagaa tacccatcag cctggactaa tataatgatg
     3421 tttttaccag gcggtcagga cttttataat cagaactcct atatttttgg cgtaacatac
     3481 aatggagttg attatgattc gactgcccct aatgctatcg cagcaccaga atgcattgat
     3541 ataaaaggag cgggtacctt taataatcac tataaaaacc ccgctgtctg tagtggaggt
     3601 cctgagcctc aattatcagt aacctttcca gctcgcgtac aactctatat taaattagca
     3661 aaaaatgcca atagagttaa taaaaacctg gtgttacctg atgaatatat cgctctagag
     3721 tttaaaggta tgagtggcgc aggagctata gaagtagaca aaaatttgac cttcaggatt
     3781 cgtggattaa ataatattca tgtccttgac tgctttgtta atgtcgcact ggaaccagct
     3841 gatggcgttg ttgactttgg taaaataaat tcccgaacaa ttaaaaatac cagcgttagt
     3901 gagacgttta gtgtagtcat gacaaaagat cctggtgcgg cctgtactga gcaattcaat
     3961 attttaggga gctttttcac tacggatatt ttgagtgatt atagccatct ggatatgggt
     4021 aatggactgc tattgaagat atttcataaa gatggaacag caacggaatt taaccgcttc
     4081 tcacaatttg cttctttttc atcgtctagt gcaccttcgg tcaccgcacc attcagggca
     4141 gaacttagtg cgaatccggc agagactgtt gttgagggac cgtttagtaa agacgtcatc
     4201 ctcaaaatca cctataacta gcatctaata caaacactaa aacgggccat caggcccgtt
     4261 atatcagtgc tctaactcca gctctcttgc ctgcatcatc tgttgacaat accaggcata
     4321 acgtgcgcaa aaccaggttc tatgttcttc atccatgtta ccagtaacgg caagaatatc
     4381 gaccgggcga ccttctgcgt gcattctggc aaaactacgc gccaggaaat cgaaattatt
     4441 taaagaatta agactatgat ccataagcac atcctccggt tatcgcatac cttttaaggc
     4501 catatgcttt ttctgatttc agtattcccc aatcagtgta gtgcctttaa acataacgtg
     4561 cgtaaatata ttgtccccga tcacactttt tagtgaaaaa taaaaaacca tattaaaaaa
     4621 ctttctcttc cagaacttct ctaacatgtg ttattattaa caaagataaa tgtaagcaag
     4681 gaaactatcc caaagtaaat attgcgtcac taaatggaca ttggagtgac atatgatttt
     4741 attcgccgac tataataccc cttatctttt cgctatttcc tttgtactgt tgatcggcct
     4801 tttagaaatt ttagcgctta tctgcggtca catgctctcc ggtgctcttg atgcacatct
     4861 tgaccattat gattccataa ccacaggtca tattagccag gcacttcatt atctcaatat
     4921 cggaagattg ccagctctcg tcgttctctg cttgctggct ggtttttttg gtcttattgg
     4981 catcctgttg cagcatgcct gcatcatggt ctggcaatca ccactgtcga acctgttcgt
     5041 ggttcctgtc agtttactgt ttacgattat tgcggtgcat tataccggta aaatagttgc
     5101 gccctggatt ccacgcgacc atagttcagc tattacagaa gaagaatata ttggtagcat
     5161 ggcattaatt accggtcatc aagccacttc aggtaatccc tgcgaaggaa aactcaccga
     5221 tcaatttggc caaatacatt atttactact ggagcctgaa gaaggaaaat ttttcactaa
     5281 aggagacaaa gtgttaatta tttgccgact ttctgcaacg cgatatttgg cggaaaacaa
     5341 tccctggcca caaatattgt aatttattaa tcaaaaggaa atttaataat ggatgatatt
     5401 gttaattctg tgccctcctg gatgtttacc gcgattattg ccgtatgcat tctgtttatt
     5461 attggaatta ttttcgccag gctctatcgt cgcgcttcgg cagagcaagc ttttgttcgt
     5521 actggtttag gtgggcaaaa agtggtgatg agcggtggcg caatcgtgat gccgatcttt
     5581 catgaaataa tccccatcaa tatgaatact ctgaagctgg aagtcagccg ctcaaccatt
     5641 gatagcctga ttacgaaaga tcgtatgcgt gtcgatgtag tagtcgcttt ctttgtacgg
     5701 gtaaaaccgt ctgtagaagg catcgccact gcggcgcaga cgttggggca acgcacgcta
     5761 tcaccagaag acttacgtat gttggttgaa gataaatttg tcgatgccct ccgtgcaaca
     5821 gctgcacaaa tgaccatgca tgagttacag gatacccgcg agaacttcgt gcagggagta
     5881 caaaatactg tcgcagaaga cctgtcgaaa aacggtcttg agctggaaag cgtttcactt
     5941 accaacttta accagacctc gaaagaacat ttcaatccga acaatgcctt tgacgccgaa
     6001 ggtttaacca aactgactca ggagacggag cgccgtcgtc gcgaacgtaa cgaagttgaa
     6061 caggatgtag aagttgcggt gcgtgagaaa aaccgtgatg cactttcgcg caagttggag
     6121 attgaacaac aagaagcgtt tatgacgctt gagcaggagc agcaggttaa aacccgtact
     6181 gccgaacaga atgcacgtat tgcggctttt gaagctgaac gtcgtcgtga agcagagcag
     6241 acgcgaattc tggctgaacg acaaattcag gaaacggaga tcgagcgcga gcaggccgtc
     6301 cgctcaagaa aggttgaggc tgaacgtgaa gttcgtatta aagagatcga acaacagcag
     6361 gtcaccgaaa tcgccaacca gacgaaatcg atcgctattg ccgccaaatc ggaacaacag
     6421 tcccaggcag aagcgcgtgc taaccttgca ctcgcagaag cagtaagtgc gcaacaaaac
     6481 gtagaaacca ctcgccagac cgcagaagcc gatcgtgcta aacaagttgc cctaatcgct
     6541 gccgcgcagg atgcagaaac caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa
     6601 gaagccgcag agatgcaggc ggcggctatc gttgagttag ccgaagctac acgtaaaaag
     6661 ggtctggcgg aagcagaagc acaacgtgcg ctgaacgatg ctatcaacgt actttctgat
     6721 gaacaaacca gccttaaatt caaactggcc ctgttgcagg cgctacctgc ggtaatagaa
     6781 aaatccgttg agccgatgaa atctattgac ggtatcaaga ttattcaggt cgatggtctg
     6841 aatcgtggca gcgctgcggg tgatgcaaac acgggtaatg tggggggcgg aaacctggcg
     6901 gaacaagcat tatcagccgc tctctcttac cgcacacagg caccgctgat tgactccttg
     6961 ctcaatgaaa ttggcgtttc aggcggctca ctggcggcat tgacttcacc cttaacctca
     7021 acaactcccg tcgccgaaaa cgtagaataa attttcagct atgaacgtcc ctggcacaaa
     7081 aagccgggga ttttttatat ctgcgttccg ctaaaaactg caaatgatca ggtcgttgca
     7141 acgttttgcg tgaccgctcg gggaaggcaa aactgcctct gggaaagcgt tgcgcgaggt
     7201 ccggcgctca tcaacaatcg gggagtcagc aaagggctga aacgggaaag cccctcccga
     7261 ggaaggggcc ttacagaagg aaagggttat gatgaagctc gtcatcatac tgattgtgtt
     7321 gttactcgta agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc
     7381 acccttgttc ctttacttta ggttgaaaaa aaatcagcgt caagaaggtt tcattaacac
     7441 atatccctgg cccaaaaagc cggggatttt ttatatctgc gttccgctaa aaggtgcaaa
     7501 tgctcaggac gttgcagcgt tttgcgtgac cgctcgggga aggcaaaact gcttcaggga
     7561 aagcgttgcg cggggtccgg cgctcatcaa caatcgggga gtcagcaagg ggctgaaacg
     7621 ggaaagcccc tcccgaggaa ggggccttat agaaggaaag ggttatgatg aagttcgtca
     7681 tcatactggt tgtgttgtta ctgttaagtt tcccgactta ctaacaactg atcagagggg
     7741 ggagaaatcc tcccttaccc ttgttccttt actttaggtt gaaaaaacaa cagcgtcaat
     7801 aggcctgcct gctacgaagc gagatctgtg aaccgctttc cggttagcct tttttatcct
     7861 gttggatctt cttgatgatg ttagtggtcg agcaaccgtc ttcgaagttg agcaccagca
     7921 cttcgccgcc gttggcccag acttctttac tcccggcaat ttcttctggt ttatagtcgc
     7981 cgcctttcac cagcagatct ggcaggatcc cggcgatcag acgctgcggc gtgtcttctt
     8041 caaacgacac tacccagtcg accgcttcca gtgcgcccag cacaatcata cgctgctcga
     8101 gtgggtttac cgggcgggaa tcccctttca gccgtttggt ggaggcatcg ctgttcacag
     8161 cgacaatcag acggtcaccc agtttgcgag catttgccag ataggagacg tgcccggcgt
     8221 gcaggatgtc aaagacaccg ttggtcatta ccaccttttc gccgcgttta cgcgctgccg
     8281 ctacagcaag cttcagttcc tcttcggtca tcacaccaaa gccggtatct gcgcgcccac
     8341 gcactgcatt ttccagctcg atcggcgaaa ccgtggaggt tcccagtttg ccgaccacca
     8401 cgccagccgc cgcattggca aagaagcagg cttcttccag cgaattaccc gctgccagcg
     8461 ttgccgccag gacgccaatc accgtgtcgc ccgcaccggt aacgtcatac acttcctgcg
     8521 cctgggttgg catatgcagc ggcgctttac ccggttgcag cagcgacata ccctgttcgg
     8581 aacgggtcac taacagagcc gagagttcgt aatcggcaat cagtttcatg ccgcgctcaa
     8641 caatctcttc ttcggtctta catttaccga caacagcttc aaattccgag agattcggcg
     8701 ttaacagcgt agcgccgcgg tagcgctcaa aatcggtacc ttttggatca atcagcaccg
     8761 gaacgcccgc tttacgcgcc agttggatca tctgctgtac gcttgccagc gcacctttgg
     8821 cgtagtcaga aagcaccagc gcgccaatcg aactcagcgc ctgattaatc cgctcgtgca
     8881 gcggctgcgg atcaacacct tcgaaacctt cttcaaaatc caggcggatc agctgttggt
     8941 tgcgggaaag tacccgtaat ttggtgatag tcggatgcgt cggtacagaa acgaagtcgc
     9001 atttgacgtt gacgtcggcc agagatttac tcagcgcacg cgccgcatcg tcaatgcccg
     9061 tcagcccgac caggcgagca ttggcaccga gagaagcgat gttcattgcc acgttagccg
     9121 cgccgcccgg acgttcttcg atggtattga ctttaaccac gggcaccggc gcttccggcg
     9181 agatacggct ggtgggaccg taccagtaac gatccagcat cacatcacca accaccatca
     9241 ctcctgcacg ttcaaactct ggcagcgtta ctttcattcc tgtctcctga gagattcaaa
     9301 atttgcgcgc gataatacca tacttcattc ttccaccagc cacttctgcc agcttgcccg
     9361 caccagttca cgctctgcgg tgaagcaatc ctccggcaca tggcccggca attcctgtaa
     9421 tgccagatga tgaagttcat cgcgcagcgt agtataagca cgggtcagcg ccatcgcttc
     9481 ctgctcttcc ataatgtcgt tttgcgccag tagttccaga atgcgcacgt tgtctgacca
     9541 gcgcgttaac ttcggttttt catgagcgta gcgcaacacc agatattggg taataaattc
     9601 gatatcggta attccgcctt catcagcttt gatatcaaag cgatcgcgat gtttattgcc
     9661 gagatgagcg cgcattttct cgcgcatttc ccgcacttcc gtttgcagag ttttaccttc
     9721 acgcggcagc gtcataatct cgcgacgcac cgcgtcaaag tgcgcggtga gctgcggatc
     9781 gccgtacact acccgcgcac gcaccagcgc ctgatgttcc cacgtccagg cctcgttttt
     9841 ctgataatcg gcaaatgctt ctgcggatgt caccagcatt cccgcggccc cggacgggcg
     9901 tagccgtgca tccacttcat acaaaatgcc ggaagaggta cgcgtactga acagatgcat
     9961 aatgcgttgc gccagacgca gataaaactg tcgcccgtcg atttcccgct cgccgtcagt
    10021 catcgcatcc attgggcaat catggaggaa gatcaggtca agatcggaac tgtagcctaa
    10081 ctcccagccg cccagcttgc cgtagccgac caccgcaaaa ccacggcctt cgcgatcgtt
    10141 gaggtgattt ggcttaccgt agcgggccac catttgaacc cacgcctgct gaacgacggc
    10201 atctatcata gcctctgcca gccaggttaa gtgatcgctc actttcatca ccggtagcgt
    10261 accagcgata tccgctgcgg cgatgcgtaa cagctgcgcc tgtttgaact gacgcagcgc
    10321 ctcaagctgt tgctcttcgt catcttccgg cacgcgcagc aaatactggc gcaactcatc
    10381 gcggtaggca tcggtcgccg tcggctggta aagggtgttt ggatcgagca attcatccag
    10441 caataatgga taacgcgcca gttggctggc aatcatcggc gacgcggcac acagagaaat
    10501 caaatgttta agcgccgcgg ggaattcact gagcaattct aaataggtgg tgcgggtaac
    10561 aatccccacc agcaaggcgg taatgcgcga cagcgtaacg gcagcgtctt cacgcgcaca
    10621 gacatcactt agcagatgcg gcatcagatg gtcgagcacc tgacgtcctc gcggcccgat
    10681 ggtgcgctta tccagctctt tgcggaaatc ggcaatcagc gttagcacct gtttgcgatc
    10741 atcctcgcta agatgcgcca gcactggcgt agtgtcatct tcctgcaacg catcctgcca
    10801 cagctcacgc cactgttccg acagcgactc ttcctgagtt tcactttcgt cgtcgccaat
    10861 caattcatta aacacccggc gcacattggt catatgtgcg gtcagcgccc cggtcagttg
    10921 cggccagtca gcaaagtcca tcgcccacgc cagccgcgca cggttaagct catcagaagg
    10981 aagcgtctgg gtttgttcgt cgttaatgct ttgcagcagg ttttccagac gccgcaggaa
    11041 cagatacgcc actcgcaatt gttcagcatc gttttcagaa agcagatgca gcgcggcaat
    11101 gacgctgagc gttggcagta aagagcgcga ctgcagcgac ggttcgcgcc caccgcgaat
    11161 gagctggaac acctgaacga taaattcaat ttcgcgaatg ccgcctgcgc cgagtttgat
    11221 attgtcggtc aaaccacgtc gacgcacttc acgggcaatc atccctttca tgttgcgcag
    11281 cgactgaatc acgctgaaat cgatgtaacg acggaaaaca aacgggcgca gcatcgcacg
    11341 caactcgtta gcatagacgc cttcgctatc gcccataatc cgcgccttga ccatcgcgta
    11401 acgctcccag tcgcgcccct gctcctggta ataatcctcc aacgcggcaa agctcagcac
    11461 cagcgggcca ctttcgccaa acggacgcag ccgcatatcc acgcgataga cgaagccatc
    11521 ctgcgttggt tgatccaaca ctttaatcag ccgctgcccc atgcgggtaa aaaactgcgc
    11581 gttatccagt tcccggcgtc cgccctgcgt acaaccatgt tccggccagg caaaaatcag
    11641 atcgatatct gaggagaaat tcagttcccc accgcccagc ttacccatgc ctaaaatcag
    11701 cagcggttgc gcttcgccct gcgcattgca cggcgttccc cactcgcggc agcaagcgtc
    11761 atacagccag tcacgcgcgg cgacaatcag cgtctccgcc agatggctga gctgctgcaa
    11821 tatgctctct tcagtaacca aagccagcgt ttgcgcccag gcgatgcgca ccataatgcg
    11881 ccgccggaat agccgcagct cgcgcattaa cccggcttcg tcactcacat tactgagcgc
    11941 ctcctgcaac catgccgcgt aatgctgcca ttcgtcggcc tgcggcggtt ggctttccag
    12001 ttccgtcagc cactctggat gcacaatcac gctgtcctgc acaaaatcac taaaagtaag
    12061 tactgacttc gcctgtgcgc taagtgattc ctcggctaaa ggctctggca gccgctcaac
    12121 aacggtctgc cagtactgct gtaacggtga agagagcggc ttcataagcg attttatcct
    12181 tgcctgatta acgttttccg ctgtgcaacc agaacggttc ctgattgttt gcctcattac
    12241 ggaaatgttc aatttcaatg cgttgcccgg tcgcaatagc gtgacgcagc ccctgccagt
    12301 tctccagcca ggcttgcgcg acgacaggat catagtaacc tgccagcagc agtattgagt
    12361 caatatcacg cgtcaggcgt ggcaactgat catggtagcg atcgcctaac ggctggcaga
    12421 aaacgctttt cagttcagcg gcatggcggg aaagatggat atcggcaaag cgtttgaagg
    12481 aatcgctgat tttaccctgc gctttggcat ctaaaaacgg ctgccaggct ttgcttacca
    12541 accattcggt caacgccagc tttgccattg ccgtttcggt agagtagacc gccgtcacgg
    12601 cagaaaccgc agaagcgatg gtcgcttcgc attgagtcag caaatcacgt aagtgagtgc
    12661 tcgctttacg cggcacaatg ccaccgaaca gcatcagtgc atggcgaaca aggccaatgg
    12721 ctgccagcac ctgctctttc gccgcatcgt tgccgcgtac ccacagttct tcatgatact
    12781 gccattgcgc taacgccagc tcgaacgccg cttccagccc ctgttccaca tcggcttttg
    12841 ccgcaacatg caaaatggtg gtcggtttga tttcacgcgc cggattgccc tgcgccagat
    12901 gatagccacg cgccgctttg ctcaggctgc cctggcgtaa tccggtttgc gataccagtt
    12961 ggttcgccag tttcagcacc gcgcgcgtgt cgccgctaag cagttccagt tccagctcac
    13021 agataggttc agcaaattcc cccgctttca cctccccctg atcgagagcg atttcaattc
    13081 ggctgtcatc gaccgccacc agccattttt cgcgataaaa atcggtgctg aacagcggtt
    13141 gtacgcggga agcgagatcg gcgggcaatt cgccgttcgg ccagacttcc gtcggtaact
    13201 gcgccaggtc gagcgtcggt tcgctcaacg ccacgttata ttccgggcgc tggtgtaagc
    13261 cgcctgtcac tcttcctgca actttcatgg tcatctcata gcgaccgttt tcgccacgaa
    13321 tacgtaagcc catatcgtgc ccacgcagcc agttatccgg cgtttcgtag taaatattca
    13381 gcaactgcac ggggtcatgg tgctcgccgc ccagcgtatt gagatggtca cgcaacgcct
    13441 caacggcact gtgattaaca ataaacttta attcgatttc ctgagccatg gcctttactt
    13501 atcggttatg tcacataagt gacgatgaac ggcgaactta atgcgatctt ttttgtcagt
    13561 agatagtatt ttgcgccaaa ttgccatgca acgggcaatt tgacgggcgt aaaagtttga
    13621 agcagtggca agacctgaca cagaggattc cgattgatgt cgaatgcttt gcgtagcgac
    13681 actgatgcca ctactatcgt tccactttcc atgacaataa cgacagcctg atgccaaaat
    13741 tacgcctgat cggattaact ttactcgcac ttagcgccac tgccgtctca cacgctgaag
    13801 aaacgcgcta tgtttctgat gaactgaata cctgggtccg tagcggtccg ggagatcatt
    13861 atcgcctcgt gggcacggtt aacgccggcg aggaagtgac cttattacaa actgacgcca
    13921 acaccaatta tgcccaggtg aaagacagct ctggccgtac cgcctggatc ccgttgaaac
    13981 aacttagcac tgagccaagc ctgcgctccc gtgtgccaga tctggaaaat caggtcaaaa
    14041 ccctgaccga taaactcacc aatatcgata acacctggaa tcagcgcacc gcagaaatgc
    14101 agcaaaaagt ggcgcagagc gacagcgtga tcaacggatt aaaagaagaa aatcagaaat
    14161 tgaaaaacga gctgattgtc gcgcagaaaa aggtcgatgc cgccagcgta cagttggatg
    14221 acaaacagcg caccatcatc atgcaatggt ttatgtatgg cggtggcgtg ctggggcttg
    14281 gcttgctgct cggtctggta ctgccacacc tgatcccaac gcgcaaacgc aaagatcgct
    14341 ggatgaacta aatcgccttc ttcgttgcac tgtcttatta ttaggaattg tgtatttgac
    14401 gaagggatga tggcgtgaag atttatctgg tcggtggtgc tgttcgggat gcattgttag
    14461 ggctaccggt caaagacaga gattgggtgg tggtcggcag tacgccacag gagatgctcg
    14521 acgcgggcta ccagcaggta ggccgcgatt ttcctgtgtt tctgcatccg caaacgcatg
    14581 aagagtatgc gctggcacgt accgaacgga aatccggttc cggttacacc ggttttactt
    14641 gctatgccgc accggatgtc acgctggaag atgatcttaa gcgtcgcgat ctgaccatta
    14701 atgcgctggc ccaggacgat aacggcgaga ttatcgaccc gtacaacggt ctgggcgatc
    14761 tgcaaaatcg tctgttgcgc catgtttccc ccgcttttgg cgaagatccg ttacgcgttc
    14821 tgcgcgtggc gcgttttgct gcgcgttatg cccacctcgg ttttcgtatt gccgatgaaa
    14881 ctctgacgct gatgcgcgag atgacccatg cgggtgaact ggaacacctg acgcctgaac
    14941 gggtatggaa agagacggaa agcgccctca ccacccgcaa tccacaggtg ttcttccagg
    15001 tactgcgcga ttgtggcgca ctgcgcgttt tattcccgga aattgacgca ctgtttggcg
    15061 ttccggcccc tgccaggtgg catccggaaa tcgacacggg tattcatacc ttaatgacgc
    15121 tctcaatggc ggcgatgctg agtccgcagg tcgatgtccg tttcgcaact ttatgccacg
    15181 atctcggtaa aggactgacg ccgccagaac tttggccgcg tcatcatggt cacggtccgg
    15241 cgggtgtgaa attagtggaa caactctgcc agcgtctgcg cgtgccaaat gaaattcgcg
    15301 atttagccag actggtggct gagtttcacg atctcatcca caccttccca atgctgaacc
    15361 caaaaactat cgtcaaattg tttgattcca tcgatgcctg gcgtaaaccg cagcgtgtcg
    15421 agcaactggc gctgaccagc gaggctgacg tgcgcggcag aaccggtttt gaatcagcgg
    15481 actacccgca aggccgttgg ttgcgcgaag cctgggaagt ggcgcagtca gtgccgacaa
    15541 aagcggtcgt tgaagcggga tttaaaggtg tggagattcg cgaggagctg acccgacggc
    15601 ggattgcggc gatagccagt tggaaggaac aacgttgccc aaagcctgac tgagtataaa
    15661 actaaacgtt aacacgaacc atcccctcgc cccttcgggg agagggttag ggtgag
//
LOCUS       DAZGXZ010000118        15675 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000118 DAZGXZ010000000
VERSION     DAZGXZ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15675
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.118"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1289)
                     /gene="uidB"
                     /locus_tag="ACHKJF_003943"
     CDS             complement(<1..1289)
                     /gene="uidB"
                     /locus_tag="ACHKJF_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP0951212.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLT"
     gene            complement(1286..3097)
                     /gene="uidA"
                     /locus_tag="ACHKJF_003944"
     CDS             complement(1286..3097)
                     /gene="uidA"
                     /locus_tag="ACHKJF_003944"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP0951213.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(3486..4076)
                     /gene="uidR"
                     /locus_tag="ACHKJF_003945"
     CDS             complement(3486..4076)
                     /gene="uidR"
                     /locus_tag="ACHKJF_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP0951214.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(4305..5072)
                     /gene="hdhA"
                     /locus_tag="ACHKJF_003946"
     CDS             complement(4305..5072)
                     /gene="hdhA"
                     /locus_tag="ACHKJF_003946"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP0951215.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(5184..6212)
                     /gene="malI"
                     /locus_tag="ACHKJF_003947"
     CDS             complement(5184..6212)
                     /gene="malI"
                     /locus_tag="ACHKJF_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP0951216.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            6387..7979
                     /gene="malX"
                     /locus_tag="ACHKJF_003948"
     CDS             6387..7979
                     /gene="malX"
                     /locus_tag="ACHKJF_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP0951217.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISSLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            7989..9161
                     /gene="malY"
                     /locus_tag="ACHKJF_003949"
     CDS             7989..9161
                     /gene="malY"
                     /locus_tag="ACHKJF_003949"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP0951218.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            9265..10266
                     /gene="add"
                     /locus_tag="ACHKJF_003950"
     CDS             9265..10266
                     /gene="add"
                     /locus_tag="ACHKJF_003950"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP0951219.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(10301..11341)
                     /gene="ydgJ"
                     /locus_tag="ACHKJF_003951"
     CDS             complement(10301..11341)
                     /gene="ydgJ"
                     /locus_tag="ACHKJF_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0951220.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            11584..11709
                     /gene="blr"
                     /locus_tag="ACHKJF_003952"
     CDS             11584..11709
                     /gene="blr"
                     /locus_tag="ACHKJF_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP0951221.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            11982..12197
                     /gene="ydgT"
                     /locus_tag="ACHKJF_003953"
     CDS             11982..12197
                     /gene="ydgT"
                     /locus_tag="ACHKJF_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP0951222.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            12283..12723
                     /gene="ydgK"
                     /locus_tag="ACHKJF_003954"
     CDS             12283..12723
                     /gene="ydgK"
                     /locus_tag="ACHKJF_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP0951223.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            12800..13381
                     /gene="rsxA"
                     /locus_tag="ACHKJF_003955"
     CDS             12800..13381
                     /gene="rsxA"
                     /locus_tag="ACHKJF_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP0951224.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            13381..13959
                     /gene="rsxB"
                     /locus_tag="ACHKJF_003956"
     CDS             13381..13959
                     /gene="rsxB"
                     /locus_tag="ACHKJF_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP0951225.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            13952..>15675
                     /gene="rsxC"
                     /locus_tag="ACHKJF_003957"
     CDS             13952..>15675
                     /gene="rsxC"
                     /locus_tag="ACHKJF_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP0951226.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQ"
BASE COUNT         3726 a         4077 c         3919 g         3953 t
ORIGIN      
        1 gtgagcggat aaaaccagat aataacgaat gccagtagca taaatccgca aggtactaag
       61 gcaattgatg tgcggatgcc cataataact tccggcgttt gcacctgatt ggcgatatat
      121 ccgcttaatc ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc gcatttacgg
      181 gtaaatgaga atagtgaata ggtgagccct tcaattcgca cgccggtcag gtattcaccg
      241 tattctacgg tatcagcttc cagtgcccac atcacagtca tagtaacgcc ctgaccaatt
      301 gaagcgatgg ccaacgcaac caacgccacc ggcagtgacc agacggaaac ccagaagaat
      361 agcagataac cgcaggttcc cagcaaagcg ccaatcagga aggtattctt tttaccgatc
      421 ctcgcgacca tccccggcac cagcggtgcc gatgccacag taccaaccag gttttgcacc
      481 agtaccagca cagtgaacag cccggtatca tttaacacat agcgcacgta gaacaacgac
      541 gaggcgctga ccgcgaaggt cgaaatcagc acacacagcg caccgatgca caacataaac
      601 agcgggcgat tccgtttcag ggtttgcaga ctgatcttca atgacggctg cgccacgata
      661 cgtaccacat tctcacgcgt cgatttgaag cagatgaagt aaagcaccat tccggcaatc
      721 gccagcacaa tcgtccagaa atggtatacc gacaccatct cttccgggct ggagttctta
      781 atgctcggtc ctatcagaaa tgccaggcag acaaaggtta atgaagcggc aatcccgcga
      841 gccgcgccca gacgggcgcg ggattgtggt tgttgggtca tcgcggtagc aagtgaacca
      901 taaggaatat tcaccaggct gtagcaaagc ccgaggccca tgtaggtcaa atatgcatac
      961 actactttgc tgctatggct ccagtcggtc ggtacccaga ataccagcac gctgaagatc
     1021 attaacggcg cagtaccgaa gagtaaaaac gggcggaatt ttccccagcg ggtattcaca
     1081 ctgtccacca ctcgtccggc aaagacgtcg gcgaaggcat cgaatacccg caccagtaac
     1141 agcatggtgc ccgccgcagc ggcaccgacg ccagcgacgt cggtgtagta actcaatagg
     1201 aagagcgccc ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct gtagccgacg
     1261 atggtgcgcc aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga
     1321 agttcatgcc agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc
     1381 gagtgaagat ccctttcttg ttaccgccaa cgcgcaatat gccttgcgag gtcgcaaaat
     1441 cggcgaaatt ccatacctgt tcaccgacga cggcgctgac gcgatcaaag acgcggtgat
     1501 acatatccag ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca
     1561 gcccggctaa cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct
     1621 gccaggccag aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga
     1681 cataccatcc gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg
     1741 tgtgagcgtc gcagaacatt acattgacgc aggtgatcgg acgcgtcggg tcgagtttac
     1801 gcgttgcttc cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt
     1861 cgttggcaat actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt
     1921 taatcgcctg taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt
     1981 ctttcggctt gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag
     2041 cagtttcatc aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt
     2101 aagggtaatg cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt
     2161 gcaccatcag cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa
     2221 agccagtaaa gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg
     2281 accggatgcc gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc
     2341 acagttcata gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag
     2401 tcccgctagt gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc
     2461 tgacatcacc attggccacc acctgccagt caacagacgc gtggttacag tcttgcgcga
     2521 catgcgtcac cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc
     2581 tgcgatggat cccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt
     2641 tttcgtcggt aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac
     2701 aaacggtgat acgtacactt ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg
     2761 gcgtatagcc gccctgatgc tccatcactt cctgattatt gacccacact ttgccgtaat
     2821 gagtgaccgc atcgaaacgc agcacgatac gctggcctgc ccaacctttc ggtataaaga
     2881 cttcgcgctg ataccagacg ttgcccgcat aattacgaat atctgcatcg gcgaactgat
     2941 cgttaaaact gcctggcaca gcaattgccc ggctttcttg taacgcgctt tcccaccaac
     3001 gctgatcaat tccacagttt tcgcgatcca gactgaatgc ccacaggccg tcgagttttt
     3061 tgatttcacg ggttggggtt tctacaggac gtaacataag ggactcctca ttaagataaa
     3121 aatactggtc aacctttaat ctgattagtt aaccaattct gtgttgcaca cagttatctg
     3181 tgagaaaccc atggaagaga aatggaaata aaaagaactg acaccgcgat caaaaaacca
     3241 aaaactgcaa tacccacagc gtgatattga tcgcattaat ctttaaaacg gcgttgacag
     3301 aagccataaa ataagcaacc gaacgttcgt tcgagtatat tgggaacgat aaactctcat
     3361 gacatatggc tacggtgaat atttttgggg gggaattctc cgatcccggc tagattgtat
     3421 tttgtacagg gaaattaaag tatgaaacgc gggcacagtt atcaccagcc cgcgaaatga
     3481 gaggatcagg atgcggttaa gataccgcca atcatcgcgc gtaatccctg agcaaacgac
     3541 gcttcacgcg ccagatcttc cgcttcgata tcggccagta caccgtaggt cagatccagc
     3601 agtaaacgtg aagtcattga cgtgttgatg tctgggctta tctcgccttt ttgctgggca
     3661 tcacgcatcc gctgggcaac aaattcagtg atcgtcatat gcttattttt aagcatggcg
     3721 gcgacctgtg ggttacgcat cccttccgcc ataatttcga ccaccagcgc ccgttgccca
     3781 aaggcttcat gggtgagaga gacaatagac tcgaccatat agtcaacaaa atgaatccct
     3841 tcaatcggtt cccggaaacg ggccagcgcc ctctcctggt cctgtaagat aatcgcctga
     3901 atcaaggctt ctttggagat gaaatggtga tagagcgtcc cgggactaat ggcgcaggat
     3961 ttacagatgg ctttcatcga ggcactgtga aatccatttt ctgaaaaaat ctctctggca
     4021 gcattgagga tccgggtccg tgtcggttgt gcttcagtct gcatgttatc catcattaaa
     4081 ccattatagt tagcgtgaat aactagttta ccgcgttgtc aggtgagcgc ctatcattgc
     4141 tgttgaatag tgcgaaggca cactctattc gtatgctttt catccagcta ttggttaacc
     4201 aatagccgtg atttggctgc cagttgttta gcagagacgt tgatgaattg cacacgctat
     4261 cccggtaaac aacacatacg gctgtttacc ggttcgttag tgtattaatt gagctcctgt
     4321 accccaccac cggagacggt gagaatttgt ccgcttaccc agctggctgc aggcgagcaa
     4381 aggaacagcg ccgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc
     4441 tgtaacattt tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt
     4501 gcccccggcg caatgccatt cacccgaata tttttttcac ccaggtcaaa tgccatattt
     4561 ctgaccagat gactggccgc agctttagat gatgcatagg aagtcatgtt tatattttta
     4621 ttttctgccg ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct
     4681 ggcgcaacaa gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga
     4741 aaatctgcca ttggcatatc aaacggttta ggaccaccgc caccggcgtt gttaaccagg
     4801 atatcaactt tacccagctt actgacggca aagtctgcca gtgcagagag ttcctgttcg
     4861 gaagtaatat cacaacggca ggcaaatgcc tgaccaccca gttgttgaat ttcgtctaca
     4921 acatggttag ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg
     4981 aatgtaatgg cgatttcttt accaatacct gcacccgcac ctgtgatgat ggcgcatttt
     5041 ccgtcgagtc tcaggttgtc agaattaaac acataaacct cctgtgaact tcagtaaaaa
     5101 taaaaggcac cgcctgtgtg ccacattttt tatcattaac cttgaggaaa ggacactacc
     5161 aggtatagca gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga
     5221 ttgcgtgaat gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtggta
     5281 ccaagttccc gcgcaggcgt gctggcccag gtaacgggta tatcatcaag tgtggttggt
     5341 gtcgcatcgg taaatgccgc cagcgaaact tgttgctcaa agtaacgatc gactccgctt
     5401 tccccgcttt gccgccctgc tttgagcaaa ccaaaccatg cccccatcgc aatagtttcg
     5461 ttatagcaaa ccacagcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc
     5521 gcggcttgct tctggctgga tgtgcactcc agcacccaat cgctgtgaaa cggcaggcca
     5581 aattttagta gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt
     5641 tgccctccca gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac
     5701 tgtgcagcct gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg
     5761 gaagcgaaaa tcaccgggat agctttttct tctgccatcc gtcgcaggtc atcgctgctt
     5821 cctgcagccc cggcaattac cacaccatcc acaccctgat tcagtaacag tgaaaaccgc
     5881 tgtgccagct gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct
     5941 tccagagctt ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg
     6001 acgatcaaac caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc
     6061 acaaatccca gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc
     6121 cctttgccac taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca
     6181 tcatgaatgg ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga
     6241 cagataaaac gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga
     6301 tttatgcctc actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca
     6361 gattgttctc tacgaggagt cgttttatga cggcgaaaac agcaccgaaa gtcacgctgt
     6421 gggagttctt ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct
     6481 gcggcattat gctcggcatt ggtagttctc ttagcagcca tgatgtcata accctgatcc
     6541 cggtcctggg caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt
     6601 ttgcttttag tttcctgcct gtcatgttct gtatcgccat cccgctgggc ctggcacgcg
     6661 aaaataaagg cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg
     6721 taaacttctg gttgaccaat aaaggcattc tgccaaccac ggatgccgcg gttctgaaag
     6781 ccaataacat ccagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg
     6841 tgatcgccgg tattatcgtc tggatgctgc atgagcgttt ccataatatc cgcctgccgg
     6901 atgcgctggc attcttcggc ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg
     6961 gccttgtcgg cctggtgatt ccattagtct ggccgatttt cgccatgggt attagcagct
     7021 tgggccatat gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac
     7081 gtctgctgtt gccgtttggt ctgcatcaca ttctggtagc attaattcgc tttaccgacg
     7141 caggcggcac gcaggaagtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg
     7201 cgcaattgag ttgcccgacc actcacggtt tttctgaaag cgccacgcgt ttcctttcgc
     7261 aaggtaaaat gcctgcgttt ctcggcggtc tgccgggtgc agcgttagct atgtatcact
     7321 gcgcgcgccc ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct
     7381 gcgtcgttgg cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc
     7441 tgtatgtcat ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg
     7501 tcaccatcgg taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg
     7561 gtctgtcaac caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact
     7621 acgtcatctt ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg
     7681 aagttgccag ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg
     7741 ttcctgcaat cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca
     7801 ttacccgtct gcgtttgtct gtgaaagata tgtcgcttgt taatgtgcag gcactgaagg
     7861 acaatcgggc aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc
     7921 cacaagttca gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag
     7981 gataagatat gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt
     8041 gggattatgt cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca
     8101 tggattttgc cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg
     8161 tatttggcta cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt
     8221 ccacccagca ttacaccgca attgatacgc agacggtggt gtatggccct tctgtcatct
     8281 atatggtttc agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca
     8341 cgcccgccta tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg
     8401 ttgctttaga gaagcaggct gacgactggt tttgcgatat gggcaagctg gaagccgtgt
     8461 tggcgaaacc agaatgtaaa attatgctcc tgtgtagccc acagaatcct accgggaaag
     8521 tgtggacgtg cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat ggtgtgcggg
     8581 ttatttccga tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga
     8641 gtaatgtggc gcggggagac tgggcgttgc tgacgtcggg ctcgaaaagt ttcaatattc
     8701 ccgcgctgac cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat
     8761 cggcactgaa aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata
     8821 tcgccgccta tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata
     8881 acctgacgta tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc
     8941 cgcaatccac ttatctggcc tggcttgatc tacgtccgtt gaatattgac gacaacgcgt
     9001 tgcaaaaagc gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg
     9061 aagaaggtcg tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag
     9121 gtgtggctgg attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa
     9181 aaaatctttg cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt
     9241 tatattcaga aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat
     9301 cgccaccttg atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat
     9361 atctcgcttc ctgcacaatc cctggaaaca ctgattcccc acgttcaggt cattgccaac
     9421 gaacccgatc tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct
     9481 cttgatgcct gtcgccgcgt ggcatttgaa aacattgaag atgcagcccg tcacggcctg
     9541 cactatgtcg agctgcgttt ttcaccaggc tacatggcaa tggcacatca gctgcctgta
     9601 gcgggtgttg tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg
     9661 caggcgaagc ttatcggcat tatgagccgg accttcggcg aagccgcctg tcagcaagag
     9721 ctggaggcct ttttagccca ccgtgaccag attaccgcac ttgatttagc cggtgatgaa
     9781 cttggtttcc cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg
     9841 catattaccg tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt
     9901 gaactgggtg cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg
     9961 gattttctcg ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc
    10021 agcaccgtag cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt
    10081 gccagcatta acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc
    10141 gttgccgcgc cagctgctgg gttatcccgc gagcaaatcc gccaggcaca gattaatggt
    10201 ctggaaatgg ctttcctcag cgctgaggaa aaacgcgcac tgcgagaaaa agtcgccgcg
    10261 aagtaacaaa atggatggtg caaattgcac catccatttt tcatgcaagg cacaaagtcg
    10321 cgcgatgttt ggcggattcg atgcccagct caatcaactc cattacctgg attgcctggc
    10381 ttgccggaac cggattttca ccatcgccat ttaacgcatc acgaatagcc gcatagtaag
    10441 ccggataatt cccaggcacc gtcaacagcg tttcttcgac acgttcctca ccttccacgc
    10501 gggtaagtac gccatcacgc atatcgtagc cccagtcttc ctgcggtaga cgctcgccat
    10561 ttttcagacg ttcttcctgt ggatcgaggc catatttcac ataactgcct cgggatccat
    10621 gcacgatata ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa atgactcgcc
    10681 gctgtggata ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact
    10741 gtgccaaatc taccgtcatg ctgaccggta aaccaaaaag cgtaatggcc tgatcaagaa
    10801 gatgtggtgc taaatcgtac cagataccgc tgcctgggcc gccctgttca cgccaacgat
    10861 cgcgcacctg cggacggaag cggtcaaaat gagactcaaa gtaagcaact tcacccagca
    10921 cgccttccgc gagtaaacct tttagcgtca agaaatcgct atcccagcga cggttatgga
    10981 atacagacag cacacgcccc aggctttttg ccagcgcatc cagctctcgc gcttgtgaca
    11041 gtgtcacggt aaagggttta tcaacgacca catgtttacc cgcctcaagc gccgctttgg
    11101 ctaacgggaa atgggtatcg ttgggtgtag gaatgacaat caggtctatg ttgggatcgt
    11161 taaacagatg cttcggctca gagacaaccg taaccgttgg ccagtcggct tttacttttg
    11221 tttcatcact gctggagatt accgccagtt ccagcccggg cgtgcccgca atcaggggcg
    11281 catggaaggt tttgctcgca taaccatacc caatcaaccc aacacggatg ttgtcgctca
    11341 tgtcattgcc tctcattgaa gtatgatggc tatttgacac tatcctttac ccacgctcaa
    11401 cagtttaata acctgccagc aataagggat gttgtttaac ttaagtcaaa aaaatagcga
    11461 attttccaac gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta
    11521 ttattgctaa gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct
    11581 gtaatgaatc gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt
    11641 cttggcgtgg cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa
    11701 cacaagtaag ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact
    11761 tgtcggatat taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga
    11821 tagtaaagcg cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg
    11881 cattcttatt attgtaataa cacaagcgtt cacttttggt gaaattttat catcactaat
    11941 aatacctacc cctttggtat taattatttt atggaccttt tatgactgtt caggactact
    12001 tattaaaatt tcgcaaaatc agttcactcg aaagtctgga aaaactctac gaccatctta
    12061 attacaccct gacggacgat caggaactga tcaatatgta tcgtgctgcc gatcaccgtc
    12121 gcgcagagct ggtttctggc gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct
    12181 ggcactatgt ccaataaaga aagtagcgat cggttataaa agccttataa taacgcccct
    12241 aaaattagat ttgcctgttt gcgtcgctat tctggagaac ctatgaccac cacaacgcca
    12301 caacgtattg gaggctggtt gcttggcccg ttggcctggc ttttagtcgc tctgttgagt
    12361 acgacgctgg cgttgttgct gtataccgct gcgttatctt ctccgcaaac atttcaaacg
    12421 ctcggcggac aagcactgac gacacaaatc ttatggggcg tttcttttat taccgctatc
    12481 gccatgtggt attacacact gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt
    12541 cctaaacact atattatctg gctgcttatt tccgtattgc tggcagtaaa agcattcgct
    12601 ttttcacccg tggaagatgg catagccgtt cgccagttgc tgtttacttt gttggcaacc
    12661 gcattgatcg tcccctattt caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg
    12721 taataacctt acagttaacc tgttgtcgcc tgctctggat taacggataa taggcggctt
    12781 ttttatttca ggccgaaaaa tgactgacta cctgttactg tttgtcggaa ctgtactggt
    12841 caataacttt gtactggtca agtttctcgg tctctgtccg tttatggggg tttccaaaaa
    12901 actggaaacc gcgatgggca tggggctggc aacaacgttt gtgatgacgc tggcgtcaat
    12961 ttgcgcctgg cttatcgata cgtggatttt gatcccactt aatctgattt acctgcgcac
    13021 cctggcattt attctggtga ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa
    13081 aaccagcccg gtgctttacc gtctgctggg gatttttttg ccgcttatca ccaccaactg
    13141 tgcagtgctc ggcgtggcgt tgctgaatat caatctcggg cacaatttct tgcagtcggc
    13201 gctgtacggt ttttccgccg ctgtcggttt ctcgctggtg atggtgctct tcgccgccat
    13261 ccgcgaacgc cttgctgtgg ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt
    13321 aattaccgca ggtcttatgt ctctggcctt tatgggcttt agtggtttgg tgaagttgta
    13381 atgaatgcta tctggattgc cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc
    13441 attctgggtt atgcctcccg ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt
    13501 gacgaaatct taccgcagag ccagtgtggt cagtgcggtt atcccggctg tcgcccctac
    13561 gcggaagcca tcagctgtaa cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct
    13621 gtgatgctaa aaattgccga gttgcttaat gtcgagccgc agccgctgga tggcgaagcg
    13681 caagagctaa cgcctgcgcg gatggtggcg gttattgatg aaaataactg tattggctgc
    13741 actaaatgta ttcaggcttg tccggtagac gccatcgttg gcgctacccg tgccatgcat
    13801 acggtaatga gtgatctctg tacgggctgc aatttatgtg ttgatccgtg cccgacgcac
    13861 tgcatctcgt tgcaaccggt cgcagaaaca cctgactcct ggaaatggga tctgaacacc
    13921 attcccgtgc gtatcattcc cgtggaacac catgcttaag ttattctctg cattcagaaa
    13981 aaataaaatc tgggatttca acggcggcat ccatccaccg gagatgaaaa cccagtccaa
    14041 cggtacaccc ctgcgccagg taccactggc gcagcgtttt gttattccac tgaaacagca
    14101 tattggcgct gaaggtgagt tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc
    14161 gcttacccgt ggtcgcggca aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac
    14221 ggctattgcg ccccactcta cggctcatcc ttcagcttta gctgaattaa gcgtgattat
    14281 tgatgccgat ggtgaagact gctggatccc gcgcgacggc tgggccgatt atcgcactcg
    14341 cagtcgcgaa gagttaatcg agcgcataca tcagtttggt gttgccgggc tgggcggtgc
    14401 aggattcccg acaggcgtta aattgcaggg tggcggagat aagattgaaa cgttgattat
    14461 caacgcggct gagtgcgagc cgtacattac cgccgatgac cgtttgatgc aggattgcgc
    14521 ggctcaggtc gtagagggta ttcgcattct tgcgcatatt ctgcagccac gcgaaattct
    14581 tatcggcatt gaagataaca aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga
    14641 ctctaacgat atttctctgc gggtgattcc aaccaaatat ccttctggcg gtgctaaaca
    14701 attaacctac attctgaccg ggaagcaggt tccacatggc gggcgttcat ccgatatcgg
    14761 cgtattaatg caaaacgtcg gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga
    14821 gccgattacc gagcgtgttg taaccctgac tggcgaagca atcgctcgcc cgggcaacgt
    14881 ctgggcacgg ctggggacgc cagtgcgtca tttattgaat gatgccggat tctgcccctc
    14941 tgccgatcaa atggtgatta tgggtggccc gctaatgggc tttaccttgc catggctgga
    15001 tgtcccggtc gtaaagatta ccaactgtct gttggctccc tctgccaatg aacttggcga
    15061 accacaggaa gaacaaagct gcatccggtg tagcgcctgt gctgacgcct gccctgcgga
    15121 tcttttgccg caacagttgt actggttcag caaaggtcag caacacgata aagctaccac
    15181 gcataacatt gctgattgca ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat
    15241 tcccctggtg caatatttcc gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga
    15301 aaagcgcgcc gcagaagcca aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga
    15361 aaaagcggct cgccttgaac gacataagag cgcagccgtt caacctgcag ccaaagataa
    15421 agatgcgatt gctgccgctc tggcgcgggt gaaagagaaa caggcccagg ctacacagcc
    15481 tattgtgatt aaagcgggcg aacgcccgga taacagtgca attattgcag cacgggaagc
    15541 ccgtaaagcg caagccagag cgaaacaggc agaactgcag caaactaacg acgcagcaac
    15601 cgttgctgat ccacgtaaaa ctgccgttga agcagctatc gcccgcgcca aagcgcgcaa
    15661 gctggaacag caaca
//
LOCUS       DAZGXZ010000119        15550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000119 DAZGXZ010000000
VERSION     DAZGXZ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.119"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            38..1171
                     /gene="ompN"
                     /locus_tag="ACHKJF_003958"
     CDS             38..1171
                     /gene="ompN"
                     /locus_tag="ACHKJF_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP0951227.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1312..1746
                     /gene="uspF"
                     /locus_tag="ACHKJF_003959"
     CDS             1312..1746
                     /gene="uspF"
                     /locus_tag="ACHKJF_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP0951228.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1922..2857
                     /gene="ttcA"
                     /locus_tag="ACHKJF_003960"
     CDS             1922..2857
                     /gene="ttcA"
                     /locus_tag="ACHKJF_003960"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP0951229.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2986..4359)
                     /gene="dbpA"
                     /locus_tag="ACHKJF_003961"
     CDS             complement(2986..4359)
                     /gene="dbpA"
                     /locus_tag="ACHKJF_003961"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP0951230.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQVAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4389..4562)
                     /gene="ynaL"
                     /locus_tag="ACHKJF_003962"
     CDS             complement(4389..4562)
                     /gene="ynaL"
                     /locus_tag="ACHKJF_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP0951231.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4764..7031)
                     /gene="adiA"
                     /locus_tag="ACHKJF_003963"
     CDS             complement(4764..7031)
                     /gene="adiA"
                     /locus_tag="ACHKJF_003963"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP0951232.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRKQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(7230..8138)
                     /gene="melR"
                     /locus_tag="ACHKJF_003964"
     CDS             complement(7230..8138)
                     /gene="melR"
                     /locus_tag="ACHKJF_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP0951233.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            8421..9776
                     /gene="melA"
                     /locus_tag="ACHKJF_003965"
     CDS             8421..9776
                     /gene="melA"
                     /locus_tag="ACHKJF_003965"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP0951234.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALHDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            9879..11300
                     /gene="melB"
                     /locus_tag="ACHKJF_003966"
     CDS             9879..11300
                     /gene="melB"
                     /locus_tag="ACHKJF_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP0951235.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(11439..12068)
                     /gene="yjdF"
                     /locus_tag="ACHKJF_003967"
     CDS             complement(11439..12068)
                     /gene="yjdF"
                     /locus_tag="ACHKJF_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP0951236.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(12191..13837)
                     /gene="fumB"
                     /locus_tag="ACHKJF_003968"
     CDS             complement(12191..13837)
                     /gene="fumB"
                     /locus_tag="ACHKJF_003968"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0951237.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYFEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(13915..15255)
                     /gene="dcuB"
                     /locus_tag="ACHKJF_003969"
     CDS             complement(13915..15255)
                     /gene="dcuB"
                     /locus_tag="ACHKJF_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP0951238.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         3778 a         3800 c         4009 g         3963 t
ORIGIN      
        1 gataaaaggc aaataaaata actaaaggat ttattcaatg aaaagcaaag tactggcact
       61 tttaattcct gccctgctcg ccgcaggtgc tgcacatgca gccgaagttt ataataaaga
      121 cggcaacaaa ttagatctgt atggcaaagt tgatggcctg cattattttt ctgataattc
      181 agcgaaagat ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa cccaaattaa
      241 cgatcaactc actggctacg gtcaatggga atacaatatt caggcaaaca acactgaatc
      301 ttcaaaaaac cagtcatgga cccgtctggc atttgccggg ctgaaatttg cagattacgg
      361 ttctttcgat tacggacgta attatggcgt aatgtacgac atcgaaggct ggaccgatat
      421 gctgcctgaa tttggcggtg actcttatac caatgcagac aactttatga ctggtcgagc
      481 caatggcgtc gcgacttatc gtaatactga tttcttcggt ctggtaaatg gtctgaactt
      541 cgcggtgcag tatcaaggta acaacgaagg agccagtaat ggtcaggaag gcaccaacaa
      601 cggacgtgat gttcgccatg aaaacggtga cggctggggt ctttccacaa catatgattt
      661 aggcatgggc tttagcgctg gtgcggcata cacctcttct gaccgcacca atgaccaggt
      721 taaccatact gcggcgggtg gtgataaagc agacgcgtgg actgctgggc taaaatacga
      781 tgctaacaat atttacctgg caaccatgta ttcagaaacg cgtaatatga ccccgtttgg
      841 cgacagcgat tatgctgttg caaacaaaac ccagaacttt gaagtcactg cacagtacca
      901 gtttgatttt ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgacctgca
      961 cgctgccggt ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc
     1021 cgatgttggc gcgacttact atttcaataa aaacatgtcc acttatgttg actataaaat
     1081 caacctgttg gatgaagatg acagtttcta cgctgccaat ggcatctcta ccgatgatat
     1141 tgtcgcttta ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt
     1201 tttctgctta ttcttcctcc tttgatctaa attaaaaatg tgaactccgt cattacacaa
     1261 aaagtgtcat ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga
     1321 acgattcttg tccctatcga tatttccgat tcagaattaa ctcaacgcgt gattagccac
     1381 gttgaggcag aggcaaagat tgatgatgca gaggttcatt tcctgacggt aataccttca
     1441 ctgccctact atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac
     1501 ctgaaagcgg aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc
     1561 gacagagtgc atgtccatgt tgaggaaggc tcgccaaaag accgcattct ggaattggcg
     1621 aagaagatcc ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat
     1681 ctgctcggtt ctaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg
     1741 cgctgacact aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg
     1801 acattttccc ctctaatccg taccatacac gccacagttt ttatatcaga tttcttatgc
     1861 tgggcgttcc ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta
     1921 aatgtcgcaa aatcaagaaa ttagtaagaa agaacaatac aacctgaaca aattacaaaa
     1981 acgtctgcgt cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga
     2041 tcgcatcatg gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg
     2101 caatttgcag caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa
     2161 gcaaccgggc ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta
     2221 caagattgtt gaagagaata cttacggtat cgtgaaagag aagattccag agggcaaaac
     2281 cacttgctca ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg caacggaact
     2341 gggggcgacg aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa cgttgttctt
     2401 aaatatgttc tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg
     2461 caaacatatc gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc
     2521 cgatgcaaaa gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca
     2581 acgtcaggtg attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga
     2641 gacgatgttc agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ctaacctgtt
     2701 cgatttcaaa ggcatcaccc acggttctga agtggttaac gggggtgatc tggcgtttga
     2761 tcgcgaagag atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt
     2821 ggatgagtta cggctgaatg tggttgaagt gaaataacca agatggcgcc cgatgcgcaa
     2881 gcgtatcggg ctactcttat ggaggccgga taagacgcgg ccagcgtcgc atccggcaat
     2941 cccgaataag atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg
     3001 cacccggcac gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc
     3061 aacagcctga cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc
     3121 tgcgccatca agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt
     3181 ggcttttttc ccgccatcga tacacaacgt tgccatttct gcttccagcg gcacaatgga
     3241 actattagct ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt
     3301 ggcccgctgt gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg
     3361 agctgtacga ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac
     3421 caccagctca agcgatttaa tatccagacc acgcgcagca acatcagtcg caaccagtac
     3481 acgggcgcta ccgttagcaa aacgtaccag ggtctgatcg cgatcgcgtt gctccaaatc
     3541 gccgtgtaat gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca
     3601 atcttttttg gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataaccg
     3661 ttgcaacaga ggaattttgc ctttgctgga tgtctcataa aattgttgtt caatgggtgg
     3721 caaagcatct gttgagtcaa tttcaatcgc caaaggatcg cgttgcactc gtccgctgat
     3781 tacggcgatg gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc
     3841 aaaacggatg acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc
     3901 atccatcacc aaagtattca gcgcatccag tgataccgtg cctttttgca agtgatccag
     3961 caaacgaccc ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat
     4021 accgaacggt tgaccaccgc acaacgtcaa aattttggta tttgggagaa aacgcgccag
     4081 ccgacgcaat tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc
     4141 ctgggtttga aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt
     4201 tttgccgctg ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag
     4261 cgcggcgacc tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg
     4321 ggcgggaggc aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg
     4381 acgcaaagtt agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc
     4441 ggttcttcat caggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact
     4501 ggaacaggat cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc
     4561 atatttccct ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca
     4621 aaaaaaagcg cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt
     4681 atgaaaacgt tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa
     4741 ttacgccttc agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca
     4801 ataatttcag tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc
     4861 agcgagcgta aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc
     4921 agcatcggga ttcctggcgg atatgggata actgagtttg ccgcgatgcg tcctggcaga
     4981 ttttcaatgg ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg
     5041 gtgatttccg ccaccggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt
     5101 tctttcagcc aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg
     5161 tcaggatatt gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag
     5221 tggcgtttga aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc
     5281 atagagaaca ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga
     5341 ccaagccagg cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca
     5401 cccattcccg gagcaaggat gctgacttta atcgggtcga gcatactcca gttatccgga
     5461 atatctttga agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg
     5521 gtcagcagtt ttgttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg
     5581 acgacttctt tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatac
     5641 agccgcgcca tcgcctgacg aaaatcaacc gcttcgtcaa tcacttcttg tgtcagtgac
     5701 aggccgctgt tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac
     5761 agcggggagg tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc
     5821 ccacgacctt cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg
     5881 gtggagtggg tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca
     5941 tagtgatcgg catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc
     6001 agacgatcgg aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg
     6061 tcataggtgc agttggtcac cacgcagtaa gacggttttt gcccagcttt gtctttagtc
     6121 agcgggcttt cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt
     6181 ggcccgataa tgccgtagcg gttgcggctt ggcaccatgt agactggttt cgcgcctgtc
     6241 agcatcaagc cttgttcgat ggatttatgg caattacggt caacgaccac gacatcgtta
     6301 tcggtcatgc aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac
     6361 caggagcgat cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca
     6421 gtatggtcaa gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcgaaacaga
     6481 ttttcaccat agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg
     6541 ccctggtggc ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg
     6601 ctgaacagtg gcggcaacag ctgcttgcgg tagcgggtca tcgcggcaac ggcgcgtccg
     6661 gcgataaagt cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga
     6721 tcccggtcca ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt
     6781 tgttggcgct catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt
     6841 tccatttgat agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa
     6901 ccatcatcaa aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc
     6961 agacgctcaa cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt
     7021 aatactttca tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc
     7081 cggccttttc gggctgtaaa gcgcgtgaaa aacaaacatt tttggaatat ttatttttat
     7141 aaacaatatg ttatgtgatt attttaattt attttcactg cttattatcg acggctaaac
     7201 tatttttttg gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg
     7261 ctaagtttgc ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa
     7321 cgactacttg aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg
     7381 ctcagtaacg cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat
     7441 tgcatgaccc gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc
     7501 acatcgttga tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta
     7561 acataaaatt gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc
     7621 agaatcggtt cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg
     7681 gcgagctggc gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca
     7741 aacgggctaa gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta
     7801 atcaggtctt tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc
     7861 atgctctgac aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg
     7921 atatgaccct gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg
     7981 aaaggcacat tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg
     8041 cgaaactcaa tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt
     8101 ttttcgtcgc tgctgcacat aaacgtatct gtattcatag atggctctct ttcctggaat
     8161 atcagaatta tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct
     8221 cttcccagag aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag
     8281 gtaacttatt accataaact cagatttact gctgcttcac gcaggatctg agtttatggg
     8341 aatgctcaac ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc
     8401 aagcaagcca ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt
     8461 cgacgatttt cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg
     8521 cgcatattgc cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc
     8581 gtaagcttat ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag
     8641 aagccttaca tgatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt
     8701 gcacggtgac tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata
     8761 cgttggggcc gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt
     8821 gcgaggacat gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg
     8881 cgatgaatac ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc
     8941 attcggtgca gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc
     9001 tgcgttaccg ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa
     9061 ccgccgacgg cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc
     9121 aggcaccgaa gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctacgaaa
     9181 tgttcaaaaa gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac
     9241 cgtggtttat taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg
     9301 agtacccgaa acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata
     9361 aaaacgcctc ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg
     9421 ctatctggac tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg
     9481 ataacctgcc acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc
     9541 agccgaccaa agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca
     9601 acgtacagac gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg
     9661 ccgcgatgat ggacccgcat actgctgccg tgctgggcat tgatgaaata tatgctcttg
     9721 ttgacgacct gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg
     9781 cgactaaacg ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata
     9841 acagaaggcg ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa
     9901 aactcagtta tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt
     9961 acctcatgta ttactacacc gatgtcgtcg ggctgtctgt gggtttggtc ggtactttgt
    10021 ttctggtggc gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg
    10081 ctacgcgatc gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact
    10141 ctgtaatctt atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct
    10201 ttgtttgcgt gacctacatc ctctggggca tgacttacac cattatggat attcccttct
    10261 ggtcgctggt tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc
    10321 cgcgtttttt tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta
    10381 attatgtcgg cggtggcgat cggggatttg gctttcagat gttcactctg gtactgatcg
    10441 ccttttttat tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag
    10501 acaatcaacc gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt
    10561 ataaaaacga tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca
    10621 acattattac cggatttgct atctattatt tctcatatgt tatcggtgat gcggatttgt
    10681 tcccctatta tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac
    10741 gcttagttaa atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt
    10801 taagctgtgg tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg
    10861 tgattgcggg tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca
    10921 tggtggcaga taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg
    10981 cttactccgt gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg
    11041 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
    11101 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
    11161 tctacttccg tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc
    11221 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
    11281 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
    11341 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
    11401 aattcacgcc gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
    11461 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
    11521 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
    11581 tccgctccct ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt
    11641 atcgccagcg ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc
    11701 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc
    11761 agcttgtcat acggattacg gctcaaccct aaccattcct gcacctcaaa accaacgggg
    11821 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaaa gaaaatgagc
    11881 gtatagagca gcggcgttaa cggataacgt ttcgcggtgg caaggagtag cggcacgaca
    11941 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
    12001 taaatcagga ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
    12061 cgggtcatgg tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa
    12121 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
    12181 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatctgc tggaagaagt
    12241 cgttaccttt gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga
    12301 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
    12361 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
    12421 cgtcggtaac ctgctgactg cggttacctt tcgctagcat gatcatgctg ccgccgtggg
    12481 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
    12541 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
    12601 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
    12661 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
    12721 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
    12781 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
    12841 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
    12901 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
    12961 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
    13021 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
    13081 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
    13141 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga
    13201 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
    13261 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
    13321 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
    13381 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgaa ataggtgtta tagacgcctt
    13441 tcgacagcgc ttcttcatca ccgccaccgg tccacacgcg ctggcctttt ttaccgacga
    13501 tgatcgcggt gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt
    13561 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
    13621 tagccgcaac ctgtttctgg tgtgccggac ggagcatgaa agaagcgtcg tgaaaggctt
    13681 gctgcgccag tagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
    13741 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
    13801 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
    13861 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
    13921 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
    13981 ccccggcaga ataaaactgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
    14041 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
    14101 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac
    14161 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
    14221 ggcatacgcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
    14281 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
    14341 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
    14401 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
    14461 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagtgcga ctacagcgat
    14521 tgcaccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag
    14581 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
    14641 ctcttcgtct ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat
    14701 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
    14761 atcaaaggtg acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact
    14821 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
    14881 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
    14941 accgcaaaga atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg
    15001 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
    15061 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ctggtggttt
    15121 acctggctga aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc
    15181 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
    15241 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
    15301 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
    15361 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattgaag cagcggcaaa
    15421 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
    15481 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
    15541 tattttttgt
//
LOCUS       DAZGXZ010000120        15440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000120 DAZGXZ010000000
VERSION     DAZGXZ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.120"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..192
                     /locus_tag="ACHKJF_003970"
     CDS             <1..192
                     /locus_tag="ACHKJF_003970"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0951239.1"
                     /translation="YFRPAEVETLLGDPTKAHEKLGWKPEITLKEMVSEMVANDLEAA
                     KKHSLLKSHGYDVAIALES"
     gene            195..1160
                     /gene="fcl"
                     /locus_tag="ACHKJF_003971"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             195..1160
                     /gene="fcl"
                     /locus_tag="ACHKJF_003971"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0951240.1"
                     /translation="MSKQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            1163..1642
                     /gene="gmm"
                     /locus_tag="ACHKJF_003972"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             1163..1642
                     /gene="gmm"
                     /locus_tag="ACHKJF_003972"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0951241.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            1639..2862
                     /gene="wcaI"
                     /locus_tag="ACHKJF_003973"
     CDS             1639..2862
                     /gene="wcaI"
                     /locus_tag="ACHKJF_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP0951242.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            2865..4301
                     /gene="cpsB"
                     /locus_tag="ACHKJF_003974"
     CDS             2865..4301
                     /gene="cpsB"
                     /locus_tag="ACHKJF_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP0951243.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            4494..5864
                     /gene="cpsG"
                     /locus_tag="ACHKJF_003975"
                     /gene_synonym="manB"
     CDS             4494..5864
                     /gene="cpsG"
                     /locus_tag="ACHKJF_003975"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0951244.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKEKTLGELVRDRMVAFPASGEINSKLAQPAEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            5919..7313
                     /gene="wcaJ"
                     /locus_tag="ACHKJF_003976"
     CDS             5919..7313
                     /gene="wcaJ"
                     /locus_tag="ACHKJF_003976"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP0951245.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            7315..8793
                     /gene="wzxC"
                     /locus_tag="ACHKJF_003977"
     CDS             7315..8793
                     /gene="wzxC"
                     /locus_tag="ACHKJF_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP0951246.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGASVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            8973..10253
                     /gene="wcaK"
                     /locus_tag="ACHKJF_003978"
     CDS             8973..10253
                     /gene="wcaK"
                     /locus_tag="ACHKJF_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP0951247.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKHSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            10250..11470
                     /gene="wcaL"
                     /locus_tag="ACHKJF_003979"
     CDS             10250..11470
                     /gene="wcaL"
                     /locus_tag="ACHKJF_003979"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP0951248.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKP
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            11481..12875
                     /gene="wcaM"
                     /locus_tag="ACHKJF_003980"
     CDS             11481..12875
                     /gene="wcaM"
                     /locus_tag="ACHKJF_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP0951249.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPSGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            13033..14028
                     /locus_tag="ACHKJF_003981"
     CDS             13033..14028
                     /locus_tag="ACHKJF_003981"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFP0951250.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            14271..15164
                     /gene="galF"
                     /locus_tag="ACHKJF_003982"
     CDS             14271..15164
                     /gene="galF"
                     /locus_tag="ACHKJF_003982"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP0951251.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         3743 a         3858 c         4275 g         3564 t
ORIGIN      
        1 tacttccgtc cggcggaagt tgaaacgctg ctcggcgacc cgaccaaagc gcacgaaaaa
       61 ctgggctgga aaccggaaat caccctcaaa gagatggtgt ctgaaatggt ggctaatgac
      121 ctcgaagcgg cgaaaaaaca ctctctgctg aaatctcacg gctacgacgt ggcgatcgcg
      181 ctggagtcat aagcatgagt aaacaacgca tttttatcgc tggccatcgt gggatggtcg
      241 gttccgccat cacgcggcag ctcgaacagc gcggtgatgt ggaactggta ttacgcaccc
      301 gcgacgagct gaacctgctg gacagccgcg cggtgcatga tttctttgcc agcgaacgca
      361 ttgaccaggt ctatctggcg gcggcgaaag tgggcggcat tgttgccaac aacacctatc
      421 cggcggattt catctaccag aacatgatga ttgagagcaa catcattcac gccgcgcatc
      481 agaacgacgt gaacaaactg ctgtttctcg gatcgtcctg tatctacccg aaactggcaa
      541 aacagccgat ggcagaaagc gagctgttgc agggtacgct ggagccgacc aacgagcctt
      601 atgccattgc caaaatcgcc gggatcaaat tgtgcgaatc ttacaaccgc cagtacggac
      661 gcgattaccg ctcggtcatg ccaaccaacc tgtacgggcc acacgacaac ttccatccga
      721 gtaactcgca tgtgatccca gcattgctgc gtcgcttcca cgaggcgacg gcacagaatg
      781 caccggatgt ggtggtgtgg ggcagcggta caccgatgcg tgaatttctg cacgtcgatg
      841 atatggcggc ggcgagcatt catgtcatgg agctggcgca tgaagtctgg ctggagaaca
      901 cccagccgat gctgtcgcac attaacgttg gtacgggcgt tgactgcact atccgcgagc
      961 tggcgcaaac catcgccaaa gtggtgggtt acaaaggccg ggtggttttt gatgccagca
     1021 aaccggatgg cacgccgcgc aaactgctgg atgtgacgcg cctgcatcag cttggctggt
     1081 atcacgaaat ctcactggaa gcggggcttg ccagcactta ccagtggttc cttgagaatc
     1141 aagaccgctt tcgggggtaa tgatgttttt acgtcaggaa gactttgcca cggtagtgcg
     1201 ctccaccccg cttgtctctc tcgactttat tgtcgagaac agtcgcggcg agtttctgct
     1261 tggcaaaaga accaaccgcc cggcgcaggg ttactggttt gtgccgggag ggcgcgtgca
     1321 gaaagacgaa acgctggaag ccgcattcga gcggctgacg atggcggaac tggggctgcg
     1381 tctgccgata acagcaggcc agttttacgg tgtctggcag cacttttatg acgataactt
     1441 ctccggcacg gatttcacca ctcactatgt ggtgctcggt tttcgcttca gagtcgctga
     1501 agaagatctg atactgccgg atgagcagca tgacgattac cgctggctga cgccggacgc
     1561 gctgctcgcc agtaacgatg ttcacgctaa cagccgcgcc tattttctcg ctgagaagcg
     1621 tgccggagta cccggattat gaaaatactg gtctacggca ttaactactc gccggagtta
     1681 accggtatcg gcaaatacac cggcgagatg gtggaatggc tggcggcaca aggtcatgag
     1741 gtgcgggtta ttaccgcacc gccttactac ccgcagtggc aggtgggtga gaactattcc
     1801 gcctggcgct acaaacgaga agagggggcc gccacggtgt ggcgctgccc gctgtatgtg
     1861 ccaaaacagc cgagcaccct gaaacgcctg ttgcatctcg gcagttttgc cgtcagcagt
     1921 ttctttccac tgatggcgca acgtcgctgg aagccggatc gcattatcgg cgtagtgcca
     1981 acgctgtttt gcacgccggg aatgcgcctg ctggcgaagc tctctggtgc gcgtaccgtg
     2041 ctgcatattc aggattacga agtggacgcc atgctggggc tgggccttgc cggaaaaggc
     2101 aaaggcggca aagtggcaca gctggcaacg gcgttcgaac gtagcggact gcataacgtc
     2161 gataacgttt ccacgatttc gcgttcgatg atgaataaag ccatcgaaaa aggcgtggcg
     2221 gcggataacg tcatcttctt ccccaactgg tcggaaattg cccgttttca acatgttgca
     2281 gacgccgatg ttgatgccct tcgtaaccag cttggcctgc cggataacaa aaaaatcatt
     2341 ctttactccg gcaatattgg tgaaaagcag gggctggaaa acgttattga agctgccgat
     2401 cgtctgcgcg atgaaccgct gatttttgcc attgtcgggc agggcggcgg caaagcgcgg
     2461 ctggaaaaaa tggcgcagca gcgtggactg cgcaacatgc aatttttccc gctgcaatcg
     2521 tatgacgctt tacccgcact gctgaagatg ggcgattgcc atctggtggt gcaaaaacgc
     2581 ggcgcggcag atgccgtatt gccgtcgaaa ctgaccaata ttctggcggt aggcggtaac
     2641 gcggtgatta ccgcagaggc ccacacagaa ctggggcagc tttgcgaaac ctttccgggc
     2701 attgcggttt gcgtagaacc ggaatcggtc gaggcgctgg tggcggggat ccgtcaggcg
     2761 ctcctgctgc ccaaacacaa cacggtggca cgtgaatatg ccgaacgcac actcgataaa
     2821 gagaacgtgt tacgtcaatt tataaatgat attcggggat aattatggcg cagtcgaaac
     2881 tctatccagt tgtgatggca ggtggctccg gtagccgctt atggccgctt tcccgcgtac
     2941 tttaccccaa gcagttttta tgcctgaaag gcgatctcac catgctgcaa accaccatct
     3001 gccgcctgaa cggcgtggag tgcgaaagcc cggtggtgat ttgcaatgag cagcaccgct
     3061 ttattgtcgc ggaacagctg cgtcaactga acaaactcac cgagaacatt attctcgaac
     3121 cggcagggcg taacactgca cctgccattg cactggcggc gctggcggca aaacgtcata
     3181 gcccggagag cgacccgtta atgctggtat tggcggcgga tcatgtgatt gccgatgaag
     3241 acgcgttccg tgccgccgtg cgtaatgcca tgccatatgc cgaagcgggc aagctggtga
     3301 ccttcggcat tgtgccggat ctaccagaaa ccggttatgg ctacattcgt cgcggtgaag
     3361 tgtctgcggg tgagcaggat acggtggcct ttgaagtggc gcagtttgtc gaaaaaccga
     3421 atctggaaac cgcccaggcc tatgtggcaa gcggcgaata ttactggaac agcggtatgt
     3481 tcctgttccg cgccggacgc tatctcgaag aactgaaaaa atatcgcccg gatattctcg
     3541 atgcctgtga aaaagcgatg agcgccgtcg atccggatct tgattttatt cgtgtggatg
     3601 aagaagcgtt tctcgcctgc ccggaagagt cggtggatta cgcggtcatg gaacgtacgg
     3661 cagatgctgt tgtggtgccg atggatgcgg gctggagcga tgttggttcc tggtcttcat
     3721 tatgggagat cagcgcccac accgccgagg gcaacgtttg ccacggcgat gtgattaatc
     3781 acaaaactga aaacagctat gtgtacgccg aatctggcct ggtcaccacc gtcggggtga
     3841 aagatctggt agtggtgcag accaaagatg cggtgctgat tgccgaccgt aacgcggtgc
     3901 aggatgtgaa aaaagtggtc gagcagatca aagccgatgg tcgccatgag catcgggtgc
     3961 atcgcgaagt gtatcgtccg tggggcaaat atgactctat cgacgcgggc gaccgctacc
     4021 aggtgaaacg catcaccgtg aaaccgggcg agggcttgtc ggtacagatg caccatcacc
     4081 gcgcggaaca ctgggtagtg gtcgcgggaa cggcaaaagt caccattgat ggtgatatca
     4141 aactgcttgg tgaaaacgag tccatttata ttccgctggg ggcgacgcac tgcctggaaa
     4201 atccggggaa aattccgctc gatttaattg aagtgcgctc cggctcttat ctcgaagagg
     4261 atgatgtggt gcgcttcgcg gatcgctacg gacgggtgta aacgtcgcat caggcgctgt
     4321 ttgtcggatg cggcgtgaac gccttatccg acctacggtt cgggtttgta gtcctgataa
     4381 gacgcggcag cgtcgcatca ggcaatgaat gcgaaaccga gatgtaaata acgacaaaaa
     4441 caaaattggc cgtttcggtc agggccaact attgcctgaa aaagggtaac gacatgaaaa
     4501 aattaacctg ctttaaagcc tatgatattc gcgggaaatt aggcgaagaa ctgaatgaag
     4561 atatcgcctg gcgcattggt cgcgcctatg gcgaatttct caaaccgaaa accattgtgt
     4621 taggcggtga tgtccgtctc accagcgaaa ccttaaaact ggcgctggca aaaggtttac
     4681 aggatgcggg cgttgacgtg ctggatatcg gtatgtccgg caccgaagag atctatttcg
     4741 ccacgttcca tctcggcgtg gatggcggca ttgaagtcac cgccagccat aatccgatgg
     4801 attataacgg catgaagctg gtgcgcgaag gggctcgccc gatcagcggt gataccggac
     4861 tgcgcgacgt ccagcgtctg gctgaagcca acgactttcc tcccgtcgat gaaaccaaac
     4921 gcggtcgcta tcagcaaatc aacctgcgtg acgcttacgt tgatcacctg ttcggttata
     4981 tcaatgtcaa aaatctcacg ccgctcaagc tggtgatcaa ctccgggaat ggcgcagcgg
     5041 gtccggtggt ggacgccatt gaagcccgct ttaaagccct cggcgcgccc gtggaattaa
     5101 tcaaagtaca caacacgccg gacggcaatt tccccaacgg tattcctaac ccgctgctac
     5161 cggaatgtcg cgacgacacc cgcaatgcgg tcatcaaaca cggcgcggat atgggcattg
     5221 cctttgatgg cgattttgac cgctgtttcc tgtttgacga aaaagggcag tttatcgagg
     5281 gctattacat tgtcggcctg ctggcagaag cattcctcga aaaaaatccc ggcgcgaaga
     5341 tcatccacga tccacgtctc tcctggaaca ccgttgatgt ggtgactgcc gcaggtggca
     5401 cgccggtaat gtcgaaaacc ggacacgcct ttattaaaga acgtatgcgc aaggaggacg
     5461 ccatctacgg tggcgaaatg agcgctcacc attacttccg tgatttcgct tactgcgaca
     5521 gcggcatgat cccgtggcta ctggtagccg aactggtgtg cctgaaagag aaaacgctgg
     5581 gcgaactggt acgcgaccgg atggtggcgt ttccggcaag cggtgagatc aacagcaaac
     5641 tggcgcaacc cgctgaggcg attaaccgcg tggaacagca ttttagccgt gaggcgctgg
     5701 cggtggatcg taccgatggc atcagtatga cctttgccga ctggcgtttt aacctgcgct
     5761 cctccaacac cgaaccggtg gtgcggttga atgtggaatc gcgcggtgat gtgccgctga
     5821 tggaagcgcg aacgcgaact ctgctgacgt tgctgaacga gtaatgtcgg atcttccctt
     5881 accccactgc gggtaagggg ctaataacag gaacaacgat gacaaatcta aaaaagcgcg
     5941 agcgagcgaa aaccaatgca tcgttaatct ctatggtgca acgcttttca gatatcacca
     6001 tcatgtttgc cggactatgg ctggtttgcg aagtgagcgg cctgtcattc ctctacatgc
     6061 acctgttggt ggcactgatt acgctggtgg tgttccagat gctgggcggc atcaccgatt
     6121 tttatcgctc atggcgcggt gttcgggcag cgacagaatt tgctctgttg ctacaaaact
     6181 ggacgttaag cgtgattttc agcgccggac tggtggcgtt caacaatgat ttcgacacgc
     6241 aactgaaaat ctggctggcg tggtatgggc tgaccagcat cggactggtg gtttgccgtt
     6301 catgtattcg tattggggcg ggctggctgc gtaatcatgg ctataacaag cgcatggtcg
     6361 ccgtagcggg ggatttagcc gccggacaaa tgctgatgga gagcttccgt aatcagccgt
     6421 ggttagggtt tgaagtggtg ggggtttacc acgacccgaa accgggcggc gtttctaacg
     6481 actgggcggg taacctgcaa cagctggtcg aggatgctaa agcgggtaag attcataacg
     6541 tctatatcgc gatgcaaatg tgcgacggcg cgcgagtgaa aaaactggtc catcaactgg
     6601 cggacaccac ctgttcggtg ctgctgatcc ccgatgtctt taccttcaac attctccatt
     6661 cacgtctcga agagatgaac ggcgttccgg tggtgccgct gtatgacacg ccgctttccg
     6721 gggttaaccg cctgctaaaa cgtgcggaag acattgtgct ggcgacgctg atcttgctgc
     6781 tgatctcccc ggtgctgtgc tgtattgcgc tggcggtgaa actcagttca cctgggccgg
     6841 ttattttccg ccagactcgc tatggcatgg atggcaagcc gatcaaagtg tggaagttcc
     6901 gttccatgaa agtgatggag aacgacaaag tggtgactca ggcgacgcag aacgatccgc
     6961 gcgtcaccaa agtggggaac tttctgcgcc gcacctcgct ggatgaactg ccgcagttta
     7021 tcaatgtgct gaccgggggg atgtcgattg tcggtccacg tccgcacgca gtagcgcata
     7081 acgaacagta tcgccagctc attgaaggct acatgctgcg ccacaaagtg aaaccgggca
     7141 ttaccggctg ggcgcagatt aatggctggc gcggcgaaac cgacacgctg gagaaaatgg
     7201 aaaaacgcgt cgagttcgac cttgagtaca tccgcgaatg gagcgtctgg ttcgatatca
     7261 aaatcgtttt cctgacggta ttcaaaggct tcgttaacaa agcggcatat tgatatgagc
     7321 ttacgtgaaa aaaccatcag cggcgcgaag tggtcggcga ttgctacggt gatcatcatc
     7381 ggcctcgggc tggtacagat gaccgtgctg gcgcggatta tcgacaacca ccagttcggt
     7441 ttgcttaccg tgtcgctggt gatcatcgcg ctggcagata cgctttctga cttcggtatc
     7501 gctaactcga ttattcagcg aaaagaaatc agtcaccttg aactcaccac gttgtactgg
     7561 ctgaacgtcg ggctggggat cgtggtgtgc gtggcggtgt ttttgttgag tgatctcatc
     7621 ggcgacgtgc tgaataaccc ggacctggca ccgttgatta aaacattgtc gctggcgttt
     7681 gtggtgatcc cccacgggca acagttccgc gcgttgatgc agaaggagct ggagttcaac
     7741 aaaatcggca tgatcgaaac cagcgcggtg ctggcgggct tcacttttac ggtggttagc
     7801 gcccatttct ggccgctggc gatgaccgcg atcctcggtt atctggtcaa tagtgcggtg
     7861 agaacgctgc tgtttggcta ctttggtcga aaaatttatc gccccggtct gcatttctcg
     7921 ctggcgtcag ttgcgccgaa cttacgcttt ggtgcctggc tgacggcgga cagcatcatc
     7981 aactatctca acaccaacct ttcaacgctg gtgctggcgc gtattctcgg cgcgagcgtg
     8041 gcaggggggt acaacctggc gtacaacgtg gccgttgtgc caccgatgaa gctgaaccca
     8101 atcatcaccc gtgtgttgtt tccggcgttt gccaaaattc aggacgacac cgaaaagctg
     8161 cgtattaact tctacaaact gctgtcggtg gtggggatta tcaactttcc ggcgctgctg
     8221 gggctgatgg tggtgtcgaa taacttcgta ccgctggtat tcggcgaaaa gtggaacagc
     8281 attattccgg tgctgcaatt gctgtgtatt gtgggtctgc tgcgctccgt gggtaacccg
     8341 attggttcgc tgctgatggc gaaagcgcgg gtcgacatca gctttaaatt caacgtattc
     8401 aaaacctttc tgtttattcc ggcgattgtt attggtggtc agatggcggg cgcaatcggc
     8461 gtcacgctcg gtttcctgct ggtgcagatt atcaacacca ttctgagtta cttcgtgatg
     8521 attaaaccgg ttcttggttc cagttatcgc cagtacatcc tgagtttgtg gctgccgttt
     8581 tacctctcgc tgccgacgct ggtggttagt tatgcgctgg gcattgtgct gaaagggcaa
     8641 ctggcgctgg ggatgctgct ggcggtgcaa atagccacgg gtgtgctggc gtttgtggtg
     8701 atgattgtgc tgtcgcgcca tccgctggtg gtggaagtaa agcgtcagtt ttgtcgcagc
     8761 gaaaaaatga aaatgctttt acgggcgggg tgagttgttg ttttcggttc cgacaacggt
     8821 tgccgggtgc agcgtaaaac gccttatcca tcctacggtt ctgtgctcgg gacctgtagg
     8881 cctgataaga cgcgtcagcg tcgcatcagg caaaaagcgg caacgaacaa aacaaacgca
     8941 gcctcacctg gcatcaaatt tatgaggaaa aaatgaaatt acttattctg ggcaaccaca
     9001 cttgcggcaa ccgcggcgac agcgccatcc tgcgcggctt acttgatgcc atcaacatcc
     9061 tcaatccaca cgccgaagtg gacgtgatga gccgctatcc ggtcagttct tcctggctgc
     9121 tcaaccgccc ggtaatgggt gatccgctgt tccttcaaat gaaacaacac aacagcgcgg
     9181 cgggcgttgt cgggcgcgtt aaaaaagtcc tccgtcgccg ctatcagcac caggtattgc
     9241 tctcacgcgt caccgacact ggcaagctgc gcaatatcgc catcgcccag ggattcaccg
     9301 acttcgtgcg cctgctgtca ggttacgacg ccattatcca ggtcggcgga tcgttttttg
     9361 tcgatctcta cggcgtgccg cagtttgaac acgcgctttg cacatttatg gcgaaaaagc
     9421 cgctgtttat gattggtcac agcgtcggcc ccttccagga tgagcaattt aaccaactgg
     9481 cgaactacgt ttttggtcac tgcgacgcgc tgatcctgcg cgaatcggtc agccttgatc
     9541 tgatgaaaca cagcaatatc accaccgcaa aagtggaaca tggcgtcgat accgcgtggc
     9601 tggtcgatca ccacaccgaa gacttcaccg ccagctatgc cgtccaacac tggctggacg
     9661 ttgccgcaca acagaaaacg gtggcaatta ccctgcgcga actggcaccg ttcgacaaac
     9721 gcctcggcac cactcagcaa gcgtatgaaa aagcctttgc cggggtggtc aatcgcattc
     9781 tcgacgaagg gtatcaggtg attgcgctct ccacctgtac gggcatcgac agctataaca
     9841 aagacgaccg catggtggcg ctcaacctgc gccagcacat cagcgatcct gcccgttatc
     9901 acgtagtgat ggatgaactc aacgatctgg aaatgggcaa aattctcggt gcctgtgaac
     9961 tcaccgtcgg tacgcgcctg cactccgcca ttatctcgat gaattttgct actccggcaa
    10021 ttgccatcaa ctacgaacat aaatccgccg ggattatgca acaactgggg ctgccagaga
    10081 tggcaattga tattcgtcat ttattagacg gcagcctgca agcgatggtt gcggatacct
    10141 taggccagct tccggcgctg aatgcgcgac ttagtgaagc cgtcagtcgt gagcgtcaga
    10201 caggaatgca gatggtgcag tccgtactgg aacgcatcgg ggaggtgaaa tgaaggtcgg
    10261 cttcttctta ttgaaatttc cgctgtcgtc ggaaaccttc gtcctcaacc aaatcactgc
    10321 gtttattgat atgggctttg aggtggagat tgtcgcgctg caaaaaggcg acacacaaaa
    10381 cacccacgcg gcatggacga aatacaacct tgccgccaga acccgctggt tacaggacga
    10441 acctacgggc aaagtggcga aactgcgcca ccgagccagt cagaccttgc gcggcattca
    10501 tcgtaaaaat acctggcagg cgctcaacct caaacgctat ggtgccgagt cgcggaacct
    10561 gattttgtct gccatttgcg gccaggtcgc aacaccattt tatgccgatg tctttatcgc
    10621 tcattttggc cctgcggggg taaccgcagc aaaactacgc gaactgggtg tcattcgcgg
    10681 caaaattgcc actatcttcc acggtattga tatctccagt cgggaagtgc tcaaccacta
    10741 cactcccgaa tatcaacaac tgtttcgccg tggcgacctg atgttaccga taagcgattt
    10801 gtgggccgga aggctgcaaa aaatgggctg cccgagggaa aaaattgccg tatcgcgtat
    10861 gggcgtggac atgacgcgtt ttagcccgcg tccggtgaaa ccgcccgcaa cgccgctgga
    10921 aatcatttcc gtcgcacgct taaccgagaa aaaaggcctg catgtggcga tcgaagcctg
    10981 ccgtcagttg aaagagcagg gcgtggcatt tcgctatcgc atcctcggca ttggcccgtg
    11041 ggaacgacgc ctgcgcacgc tcatcgaaca atatcaactg gaagatgtgg tggagatgcc
    11101 gggctttaaa ccgagccatg aagtgaaagc gatgctcgac gatgcggatg tcttcctctt
    11161 gccatcggtt acaggtgcgg acggcgatat ggaaggcatt ccggtggcgc tgatggaagc
    11221 gatggcggtc ggcattccgg ttgtttctac tctacatagc ggaataccgg aactggtgga
    11281 ggccgataaa tccggctggc tggtgcctga gaacgatgct cgcgcactgg cgcaacgcct
    11341 ggcggcattt agccaactgg acaccgacga actggctccg gtcgtcaaac gtgcacgcga
    11401 aaaagtcgaa cacgatttta accagcaggt gattaatcga gaactcgcca gcttgctgca
    11461 ggctttatag aggagaacgt atgccattta aaaaactctc ccgacgcacc ttcctgacgg
    11521 caagctccgc gcttgccttc ctccatacac ctttcgcccg tgcacttcct gcccggcaaa
    11581 gcgttaacat taacgactac aaccctcacg actggatcgc ctcatttaaa caagccttca
    11641 gcgaagggca tacggtcgtc gtgcctgccg gattggtttg tgacaatatc aacaccggca
    11701 tcttcattcc ttctggcaaa acgttacaca tccttggaag cctgcgcggc aacggcagag
    11761 ggcgatttgt cttacaggat ggcagccagg tgacagggga ggagggcggc agtatgcata
    11821 acatcacctt ggatgtgcgc ggctctgact gcaccatcaa agggctggcg atgagcggct
    11881 ttggcccggt aacgcagatt tatattggtg gcaaaaacaa acgggtcatg cgcaacctga
    11941 ccatcgataa cctcactgtc agccacgcta attacgccat cttacgccag ggatttcata
    12001 accagattat cggtgccaac atcaccaact gtaagttcag tgatttacag ggcgacgcca
    12061 ttgaatggaa cgtggcaatt aacgacagtg atattttgat ctctgaccat gtcatcgagc
    12121 gcatcaactg taccaacggc aaaatcaact ggggaatcgg cataggcctt gcggggagta
    12181 cttatgataa taactacccg gaagaccagg cagtgaaaaa ctttgtcgtg gcgaatatca
    12241 cgggatcgga ttgtcggcag ttgatccatg ttgaaaatgg taaacatttt gttattcgta
    12301 atatcaaagc ccgcaatatc acgccggatt tcagtaagaa agcaggtatt gataacgcca
    12361 cagtcgctat ttacggttgt gacaatttcg tgattgataa tattgaaatg attaatagcg
    12421 ccgggatgtt aatcggctat ggggtaatta aaggcaaata tctctcgata ccacaaaatt
    12481 tccgagtgaa taatattcag ctggataata cccaccttgc ttataaattg cgcggcatcc
    12541 aaatctccgc cgggaatgct gtctcctttg tggcactgac taacattgag atgaagcgtg
    12601 cgtcgcttga gctacacaat aaaccgcaac acctttttat gcgaaatatc aatgtgatgc
    12661 aggaatcctc agttggacca gcattgagca tgaacttcga catgcgcaaa gacgttcgcg
    12721 gcgtctttat ggcgaaaaaa gaaacactgc tgtctcttgc aaatgttcat gcggtgaatg
    12781 aaagagggca aagctccgtc gatatcgaca ggattaatca ccatattgtt aatgtggaaa
    12841 agattaactt tagattgccg gaacggaggg agtagatttg cgaccattca taaacttact
    12901 ggatataatg ttctaaaaga ttataatcct cacactaact gttaaagctg gggctgggca
    12961 gcattgacta ctcagggaag gtactcatct ttatcttgtg tttctttcgt aacaaccaga
    13021 ctaaagtgat ttatgaacga taacgttttg ctcataggag cttccggatt cgtaggaacc
    13081 cgactacttg aaacggcaat tgctgacttt aatatcaaga acctggacaa acagcagagc
    13141 cacttttatc cagaaatcac acagattggc gatgttcgtg atcaacaggc actcgaccag
    13201 gcgttagccg gttttgacac tgttgtacta ctggcagcgg aacaccgcga tgacgtcagc
    13261 cctacttctc tctattatga tgtcaacgtt cagggcaccc gcaatgtgct ggcggccatg
    13321 gaaaaaaatg gcgttaaaaa tatcatcttt accagttccg ttgctgttta tggtttgaac
    13381 aaacacaacc ctgacgaaaa ccatccacac gaccctttca accactacgg caaaagtaag
    13441 tggcaggcag aggaagtgct gcgtgaatgg tataacaaag caccaacaga acgttcatta
    13501 accatcatcc gtcctaccgt tatcttcggt gaacgcaacc gcggtaacgt ctataacttg
    13561 ctgaaacaga tcgctggcgg caagtttatg atggtgggcg cagggactaa ctataagtcc
    13621 atggcttatg ttggaaacat tgttgagttt atcaagtaca aactgaagaa tgttgccgca
    13681 ggttatgagg tttataatta cgttgataag ccagatctga acatgaacca gttggttgct
    13741 gaagttgaac aaagtctgaa caaaaagatc ccttctatgc acttgcctta cccactagga
    13801 atgttgggtg gatattgctt tgatatcctg agcaaaatta cgggcaaaaa atacgctgtc
    13861 agctctgtgc gcgtgaaaaa attctgcgca acaacacagt ttgacgcaac gaaagtgcat
    13921 tcttcaggtt ttgtggcacc gtatacgctg tcgcaaggtc tggatcgaac tctgcagtat
    13981 gaattcgttc atgccaaaaa agacgacata acgtttgttt ctgagtaatg cagacgagtc
    14041 agagaaaaaa ggaccaggtt ggactacgat tattcggaaa gaggtactga ttaaattatc
    14101 agtatgcgga attccgagta atccgagtaa cccagatgct gatggtgtgt agtgttatcc
    14161 taaggtttaa gcaaagttat gcggaactaa gctttggtat atagccataa cgtttggcta
    14221 acctatggca ataatttacg cactatcagc cacttagagg taggaaaaga atgacgaatt
    14281 taaaagcagt tataccggta gcaggtcttg ggatgcatat gttgcctgcc actaaggcga
    14341 ttcccaaaga gatgctaccg atcgtcgaca agccaatgat tcagtacatt gttgacgaga
    14401 ttgtggctgc agggatcaaa gaaatcctcc tggtaactca cgcgtccaag aacgcagtcg
    14461 aaaaccactt cgacacctct tatgaattag aatctctcct ggaacagcgc gtgaagcgtc
    14521 aactgctggc ggaagtacaa tctatctgtc cgccgggcgt gaccattatg aacgtgcgtc
    14581 agggtgaacc tttaggtttg ggccactcca ttttgtgtgc acgccccgcc attggcgaca
    14641 acccatttgt cgtggtgctg ccggacgttg ttatcgatga tgccagcgcc gacccgctgc
    14701 gctacaacct tgctgccatg attgcgcgct tcaatgaaac gggccgcagc caggtgctgg
    14761 caaaacgtat gccgggtgac ctctctgaat actctgtcat ccagaccaaa gagccgctgg
    14821 accgtgaagg caaagtcagc cgcattgttg aatttatcga aaaaccggat cagccgcaga
    14881 cgctggactc agacatcatg gccgtaggtc gttatgtgct ttctgccgat atttggccgg
    14941 aactggagcg cactcagcct ggtgcatggg gacgtattca gctgactgat gctattgccg
    15001 aactggcgaa aaaacagtcc gttgatgcaa tgctgatgac tggtgacagc tacgactgcg
    15061 gtaaaaaaat gggctatatg caggcgtttg tgaagtatgg actgcgcaac ctgaaagaag
    15121 gggcgaagtt ccgtaaaggc attgagaagc tgttaagcga ataatgaaaa tctgaccgga
    15181 tgtaacggtt gataagaaaa ttataacggc agtgaggatt cgtggcgaaa gtaatttgtt
    15241 gcgaatattc ctgccgttgt tttatataaa taatcagaat aacaacgagt tagcaatagg
    15301 attttagtca aagttttcca ggattttcct tgtttccaga gcggattggt aagacaatta
    15361 gcgtttgaat ttttcgggtt ttgcgcgagt gggtaacgct cgtcacatcg taggtatgca
    15421 tgcagtgctc tggtagctgt
//
LOCUS       DAZGXZ010000121        15164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000121 DAZGXZ010000000
VERSION     DAZGXZ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.121"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1144
                     /gene="dsdA"
                     /locus_tag="ACHKJF_003983"
     CDS             <1..1144
                     /gene="dsdA"
                     /locus_tag="ACHKJF_003983"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP0951252.1"
                     /translation="LAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQLLLK
                     KDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQYSIA
                     VGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGVAVEE
                     GRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYLPCGV
                     GGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNLTAAD
                     GLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMAGPQR
                     VCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(1252..2790)
                     /gene="emrY"
                     /locus_tag="ACHKJF_003984"
     CDS             complement(1252..2790)
                     /gene="emrY"
                     /locus_tag="ACHKJF_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP0951253.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2790..3953)
                     /gene="emrK"
                     /locus_tag="ACHKJF_003985"
     CDS             complement(2790..3953)
                     /gene="emrK"
                     /locus_tag="ACHKJF_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP0951254.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4369..4983
                     /gene="evgA"
                     /locus_tag="ACHKJF_003986"
     CDS             4369..4983
                     /gene="evgA"
                     /locus_tag="ACHKJF_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP0951255.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            4988..8581
                     /gene="evgS"
                     /locus_tag="ACHKJF_003987"
     CDS             4988..8581
                     /gene="evgS"
                     /locus_tag="ACHKJF_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP0951256.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFLALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8637..9782)
                     /gene="yfdE"
                     /locus_tag="ACHKJF_003988"
     CDS             complement(8637..9782)
                     /gene="yfdE"
                     /locus_tag="ACHKJF_003988"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP0951257.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9856..10800)
                     /gene="yfdV"
                     /locus_tag="ACHKJF_003989"
     CDS             complement(9856..10800)
                     /gene="yfdV"
                     /locus_tag="ACHKJF_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP0951258.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10870..12564)
                     /gene="oxc"
                     /locus_tag="ACHKJF_003990"
     CDS             complement(10870..12564)
                     /gene="oxc"
                     /locus_tag="ACHKJF_003990"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP0951259.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12618..13868)
                     /gene="frc"
                     /locus_tag="ACHKJF_003991"
     CDS             complement(12618..13868)
                     /gene="frc"
                     /locus_tag="ACHKJF_003991"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP0951260.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14381..15013)
                     /locus_tag="ACHKJF_003992"
     CDS             complement(14381..15013)
                     /locus_tag="ACHKJF_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP0951261.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         4406 a         3398 c         3180 g         4180 t
ORIGIN      
        1 tctggcaaaa gcatttcctg aaactgctgc cactgggggg attattgaat cagaactggt
       61 tgccattcca gctatgcaaa aacggctgga aaaagaatat cagcaaccga tcagcgggca
      121 actgttactg aaaaaagata gccatttgcc catttccggc tccataaaag cacgcggcgg
      181 gatttatgaa gtcctggcac acgcagaaaa actggctctg gaagcggggt tgctgacgct
      241 tgatgatgac tacagcaaac tgctttctcc ggagtttaaa cagttcttta gccaatacag
      301 cattgctgtg ggctcaaccg gaaatctggg gttatcaatc ggcattatga gcgcccgcat
      361 tggctttaag gtgacagttc atatgtctgc tgatgcccgg gcatggaaaa aagcgaaact
      421 gcgcagccat ggcgttacgg tcgtggaata tgagcaagat tatggtgttg ccgtcgagga
      481 aggacgtaaa gcagcgcagt ctgacccgaa ctgtttcttt attgatgacg aaaattcccg
      541 cacgttgttc cttgggtatt ccgtcgctgg ccagcgtctt aaagcgcaat ttgcccagca
      601 aggtcgtatc gtcgatgctg ataaccctct gtttgtctat ctgccgtgtg gtgttggcgg
      661 tggtcctggt ggcgtcgcat tcggacttaa actggcgttt ggcgatcatg ttcactgctt
      721 ttttgccgaa ccaacgcact ccccttgtat gttgttaggc gtccatacag gattacacga
      781 tcagatttct gttcaggata ttggtatcga caaccttacc gcagcggatg gccttgcagt
      841 tggtcgcgca tcaggctttg tcgggcgggc aatggagcgt ctgctggatg gcttctatac
      901 ccttagcgat caaaccatgt atgacatgct tggctggctg gcgcaggaag aaggtattcg
      961 tcttgaacct tcagcactgg cgggtatggc cggacctcag cgcgtgtgtg catcagtaag
     1021 ttaccaacag atgcacggtt tcagcgcaga acaattgcgt aatgccactc atctggtgtg
     1081 ggcgacggga ggtggaatgg tgccggaaga agagatgaat caatatctgg caaaaggccg
     1141 ttaataacgt ttcaacgcag catcgcaatc ctttccctgg gtgagcgatg ctgccgatgg
     1201 cgcagactta agatccccgc tcttacccac tataaccccc tccttttatc atcacccaac
     1261 gcctttcgct gtaaacggcg gtttcgcaaa ccaaaccaaa accgtaagta agataaaagc
     1321 aatagccgcc atacggaaaa tctcatttgc agaaatagaa agtgactgct gggttatttc
     1381 attattaatt tcattaagaa ctcctgaaag cgaaccatag tatttatcca taatttgtga
     1441 cgatgaatta aacacggggt taaattgatc gatggttgct gtcaactgac tatggtgtaa
     1501 cgattcgcgt cgtccccaca gcgtcattgt caacgacgta ccaactgatc ctgacaaggt
     1561 acgaaaaaaa ttactcatac tcgaggcatt ggcaaattta ttatctggca agcctgaaaa
     1621 cgaaatcgtt gttaagggta aaaagaaaca ggcaacggcg aatccctgga aaaactgtgg
     1681 caaaatgatg cctgtaaaat caatcgttgg cataaatgtc acagaacgcc agtaatagca
     1741 aaccgcatac atcaaaaaac taaatgtcac taacaaccgc atgtctattt tgttgccata
     1801 acgtcctatc aaaggtgaaa ttaatagtgg catgatgccg atgggcgcat aagcaagtcc
     1861 ggcccatatc gcattatacc ccatcgtttc ctggagtaac tgcggcataa ggacgatcgc
     1921 tccagagtaa aataaatacg cgcatgtgat actcacaata ccaatggtga agttacggga
     1981 cttaaacaaa ctgagatcaa gaatcgggtt ctctgaggtc gactcccaaa tgactaaaga
     2041 gatcagagaa ataactgata ctactgttaa tattattatt gtactcgagt tgaaccaatc
     2101 cagatcgcgc cctttatcaa gcataatttg caagccacca acaccgagca ctaacagggt
     2161 cagtcctggt agattcattt tgaccggtga agtttcagtt tctcttcctt taagtaaggt
     2221 taagcataat gtcaggacga taatccccat agggacattg attaaaaata tccaacccca
     2281 gctaaagtta tcgcaaatat aaccgcccaa tatcggccca catatcggag cgataatcac
     2341 ggtcattgac cataatgcca gagcaaatgt tcttttttct ggtggataat tccttaataa
     2401 taaactctgt gacagtggaa ttaacggccc cgccattaat ccctgaacga ctctaaaaaa
     2461 tatcagcaca tcaagattgg tcgataaact acacattaag gaagacagcg aaaaaaaagt
     2521 gactgaaagt aaaaataacc ttaattcgcc tattctttgt gccagcctgc cggtaacagg
     2581 gatcgcaatg gcatttgcta cgccaaacga ggtgataacc caggtgcctt cgtctgttga
     2641 tgctcccaga aagccagata ttgtcggtat tgcgacgtta gaaatagtgg aatccaacat
     2701 ttgcataaac gtcgctaatg acaatgcaat agtaacgcac cataacgtcc caccggttaa
     2761 tggtgccgga gtcgatttag tgattgccat taaagttgtc cattatgcga aataatgttg
     2821 ctaatttctt tttctatcgg actggtatcg ataactaaag ccttactggt ataagccggc
     2881 atggacgtca cggttgaagc cagatcaggc atctcagcaa tgtcttcgtt cttcgtatca
     2941 atagttgcag tcatcgataa accaatgcgt aaggggtgtt ccatgagttc ttttggatca
     3001 agagaaactt caaccggtac acgctgaacg attttgatcc agttccctgt cgcattttgt
     3061 gcaggtaata aggagaacgc attgccggtt cccatattga tccctgtcac ccgaccatga
     3121 aacacaacat tttcaccata aagatcgctg ataatattga ctgattgacc aatccgtaca
     3181 tccgtgagtt gtgtttcttt aaagttggca ttaacccaca tttgacgtgc cggtacgaca
     3241 gccattaacg attgtccggg actcactgtt tcgccgacct gaacactccg ctgggcaata
     3301 tagccagtaa ccggactcct tatatccgta cgtttaagcg ccaaccaggc ttctttagtt
     3361 gcatccgccg cttccacgac ttgtggctga cggtttaatg gtgtgttcat tactaaagct
     3421 ttattcgctt tataagcctg gatagcggca ttcagtgccg ctttgctact tattaacgta
     3481 tctttggtat gctccagcgt ttcttttgaa ataaccccct gcttcgctaa cggtactcga
     3541 cggttataat cttctaaaga ttgttgatac tgaatacgtg ctgaagcgac ttcggcactg
     3601 tattgtttat cctgtaagta cagtttattc gtttgccgaa caatatttgc cagattattt
     3661 ttagctttat tgagtgcgat agtggcatca gttttatcca gtgaaactaa aatgtcacct
     3721 tgtcgaacgt agttcgtatc tttatgatta acgacagtga cactacctga gacttgtgca
     3781 gaaattggat ctgcattccc cgtgacatag gcgtcatctg tactaatcat gtcttctaat
     3841 tccattgacc aataggcata ggcacctgaa aacgcaataa ataaaactac cgccaataaa
     3901 gaaaagtatt ttcttctgtt agaatgtttt ttatttgaat taatctgttc cactattatc
     3961 tctcatttct catagatgaa atttatgaat tgaatgaaaa gagcattcat tcttcatatc
     4021 aaacacagtg catgtgttgc acattatccc aggaacgttg gaggcctaat ttaaatgagg
     4081 tgcaaaaaaa acaacagagg actaaaccgt ggcttttgca atacaattct tacgcctgta
     4141 ggattagtga gaagacttat agtgctaact tgaaactata aatcatcggt ataatccctg
     4201 attttattgt tgacatttca tttatgccga ctatttatat ggtatacttg tcgaattatc
     4261 ttaaaggaag ctcagatttt cttattttta ttgagaaaat gagatgacgc cttatgtctg
     4321 tattactaca gggagaaggg agatgcttca ttgcaaaggg aataatctat gaacgcaata
     4381 attattgatg accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat
     4441 attgaaatct tagcggagtt gactgaaggg ggaagcgccg ttcagcgggt ggaaacactt
     4501 aagcctgata tcgtcatcat tgatgtcgat atccccggag ttaacggtat ccaggtgtta
     4561 gaaacgctga ggaagcgcca atatagcgga attattatta ttgtctccgc taaaaatgac
     4621 catttttacg ggaaacattg tgctgatgct ggcgctaatg gattcgtgag taaaaaagaa
     4681 ggcatgaaca atatcattgc ggctattgaa gctgcaaaaa atggctactg ctatttcccc
     4741 ttctctctca accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta
     4801 tcgaaacaag aaattagtgt catgcggtat attcttgatg gcaaggataa taatgacatt
     4861 gctgaaaaaa tgttcatcag caacaaaact gtcagcactt ataaaagtcg cctgatggaa
     4921 aaattagaat gtaaatcact gatggatctt tacacattcg cgcaacgtaa caaaatcggc
     4981 taatcacatg aagtttttac cctatttttt tcttctctgt tgtggtcttt ggtcgaccat
     5041 aagtttcgca gacgaagatt acatcgaata tcgtggcatc agtagtaaca accgtgtcac
     5101 acttgatcca gtacgtctga gcaacaagga attacgttgg ttagcgagca aaaaaaatct
     5161 tgtgattgca gtacacaagt cccaaacggc taccttgttg cataccgatt cgcagcaaca
     5221 ggttcgtggt attaatgctg attatttaaa tcttttaaaa agagcgttaa atatcaaatt
     5281 aacactccgg gaatacgccg atcatcaaaa agcaatggac gcgcttgcag aaggtgaagt
     5341 cgatatagtg ttatcacatt tagttacttc gccgcctctt aatagtgaca ttgctgcaac
     5401 caaaccattg ataattacct ttctggcgct ggtaaccacc cttcacgact caatgcgacc
     5461 gcttacctca ccaaaaccag taaatattgc tcgggtagca aattaccccc cagacgaggt
     5521 aattcatcaa tcgtttccaa aagcaacaat tatctctttt acaaatttat atcaggcatt
     5581 agcatccgta tcagccggac agaatgatta ctttattggt agtaacatca ttaccagcag
     5641 tatgatttcc cgctatttca ctcactcctt aaatgtagtg aaatattata actcgccgcg
     5701 tcaatataat tttttcttga ccagaaaaga atccgtcatt cttaatgaag tactcaatag
     5761 atttgttgat gctttaacaa atgaagttcg ctatgaagtt tcacaaaatt ggcttgatac
     5821 aggaaacctg gcctttctga acaaaccatt agaactcact gaacatgaaa aacagtggat
     5881 taagcaacat cccgatttaa aggtgctgga aaatccttac tctcctccct attctatgac
     5941 ggatgaaaat ggctcggttc ggggcgttat gggggatatt cttaatatta ttaccttgca
     6001 aacaggttta aatttttctc cgatcaccgt ttcacacaat atccatgccg gaacacagct
     6061 taaccccggt ggatgggata taatacctgg cgctatttat agtgaagatc gagaaaataa
     6121 tgtttcattt gcagaagctt tcataacaac gccatacgtt tttgtcatgc aaaaagcacc
     6181 tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc attccatatt attatgagct
     6241 gcattcgcaa ttaaaagaga tgtatccgga ggttgaatgg atacaggtcg ataatgccag
     6301 cgctgcattt cacaaggtta aggaaggtga acttgatgct ctggtcgcga cacagttaaa
     6361 ttcgcgttac atgatcgatc attactatcc taatgaactt tatcattttc ttattcctgg
     6421 cgttccgaat gcatcgcttt cgttcgcttt tcctcgcgga gaaccggaac ttaaggatat
     6481 tattaataaa gcactgaatg caattccccc aagcgaagtt ctgcgcctga cggaaaaatg
     6541 gattaaaatg cccaatgtga ccattgacac atgggaccta tatagcgagc aattttatat
     6601 tgttacgaca ttatccgttt tattagttgg cagtagcctt ttatggggat tctacctgtt
     6661 acgctcagtt cgtcgtcgta aagtcattca gggtgattta gaaaaccaaa tatcattccg
     6721 aaaagcactc tcggattcct taccgaatcc aacttatgtt gtaaactggc aaggtaatgt
     6781 cattagtcat aatagtgctt ttgaacatta tttcactgcg gattactaca aaaatgcaat
     6841 gttaccatta gaaaacagtg actcaccctt taaagatgtt ttttctaatg cgcatgaagt
     6901 cacagcagag acgaaagaaa atcgaacaat atacacacag gtatttgaaa ttgataatgg
     6961 catcgagaaa agatgcatta atcactggca tacattatgc aatcttcctg caagtgacaa
     7021 tgcagtatat atttgtggtt ggcaagatat tactgaaacg cgtgatctaa ttaatgcact
     7081 cgaggtagaa aaaaataaag cgataaaggc taccgtagca aaaagtcagt ttctggcaac
     7141 gatgagtcac gaaataagaa caccaataag ctctattatg ggcttcctgg aacttctgtc
     7201 gggttctggt cttagcaagg agcaacgggt ggaggcgatt tcacttgcct acgccaccgg
     7261 acaatcactc ctcggcttaa ttggtgaaat ccttgatgtc gacaaaattg aatcgggtaa
     7321 ctatcaactt caaccacaat gggtcgatat ccctacttta gtccagaaca cttgtcactc
     7381 tttcggtgcg attgctgcaa gcaaatcgat cgcattaagt tgcagcagta catttcctga
     7441 tcattacctg gttaagatcg accctcaggc gtttaagcag gtcttatcaa atttactgag
     7501 caatgctctc aaatttacca ccgagggggc agtaaaaatt acgacctccc tggttcacat
     7561 tgatgacaac cacgctgtta tcaaaatgac gattatggat tctggaagtg gattatcgca
     7621 ggaagaacaa caacaactct ttaaacgcta cagccaaaca agtgcaggtc gccagcaaac
     7681 aggatctggc ttaggcttaa tgatctgcaa agaattaatt aaaaatatgc agggcgattt
     7741 gtcattagaa agtcatccag gcataggaac aacatttacg atcacaatcc cggtagaaat
     7801 tagccagcaa gtggcgactg tcgaggcaaa agcagaacac cccatcacac tacctgaaaa
     7861 gttgagcata ttaatcgcgg atgatcatcc gaccaacagg ctattactca aacgccagct
     7921 aaatctatta ggatatgatg ttgatgaagc cactgatggt gtgcaagcgc tacacaaagt
     7981 cagtatgcaa cattatgatc tgcttattac tgacgttaat atgccgaata tggatggttt
     8041 tgagttgact cgcaaactcc gtgagcaaaa ttcttcctta cccatctggg ggcttacagc
     8101 caacgcacag gctaacgaac gtgaaaaagg gttaagttgc ggcatgaact tatgtttgtt
     8161 caaaccgttg accctggatg tactgaaaac acatttaagt cagttacacc aggttgcgca
     8221 tattgcacct cagtatcgcc accttgatat cgaagccctg aaaaataata cggcgaacga
     8281 tctacaactg atgcaggaga ttctcatgac tttccagcat gaaacgcata aagatctacc
     8341 cgctgcgttt caagcactag aagctggcga taacagaact ttccatcagt gtattcatcg
     8401 catccacggt gcggctaaca tcctgaattt gcaaaagttg attaatatta gccatcagtt
     8461 agaaataaca cctgtttcag atgacagtaa gcctgaaatt cttcagttgc tgaactctgt
     8521 aaaagaacac attgcagagc tggaccagga gattgctgtt ttctgtcaga aaaatgacta
     8581 aatagcggct cccacaatgt tcaaatgtgg gagctattta ccagcacatc ttacatttat
     8641 gatgagaact cctggcgaat ttgttcccca tgctggtcga gcgttgccgc tcccggcata
     8701 acatgcgggt ccgcgcagcc gctgattttt atcggattac ccggcatcat tattcccccg
     8761 gcttcaatca acatatttct cgcctgagtt tgtggcaatt taatggcctc agccacactt
     8821 aataacggcg cgacgggtac accaacttca tgtattctgg ctaaccaaac ttcagctgcc
     8881 tgcgttttta acgtccgctc aatatattgt ttaagaatag cctggttttg tacgcgtaaa
     8941 atattgctgc taaatcgggg atcattaacc agttccgtaa gctccagtgc ctggcataac
     9001 gcagaaaaaa gcttgtcatt accacaacaa atcgtaatcg gcttatcctg agtattgaaa
     9061 acatcaaaag gtgccatgta gggatggcga tttcccagac gttgtggtga cttcccagtt
     9121 gcgatatatg ccatcagacc atgctccaga aaactcagcg tggcatcaaa catcgctata
     9181 tcgacatgcg cccctctctg gctcttttcg cggccataaa gtgcactcac tattccgctg
     9241 aataaataga caccgccgca tagatccgca agagaggtac caacgcgcac tggcggagca
     9301 tcaggatatc ctgtttccat cattatcccg ctcattgcct gaatgatggt atcgtaggca
     9361 ggagcatctt ttagcggacc ggtatgtccg aaacctgacg atgaagcata tatgaggcgc
     9421 gggttgattt cttgaagcgt ttcccatgaa aaccccagtt tttccattgt acctgggcga
     9481 aaattctcag ctaatacatc agcttgtttg agcatattta taaatatact tttatcgtga
     9541 tcattcttta aatcaagaac cacactctct ttgccatgat taataaaact gtaatagagt
     9601 gactgtccat ccacataggg accaaatgtg cgggtatcat caccatgacc cggcggctca
     9661 actttaatta cccttgcgcc catattacaa agaagttgag ttccgaaagg tccattaagg
     9721 acatgtgtca tatcgataac taataagcct tcaaacggcc ctttgctttc attatttgtc
     9781 atttgcttat gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt tatgtaaaat
     9841 caataacatg attaattatg aaaccagtcg actgacataa atccacaagg gagccgtgac
     9901 gacaaagccc agtacgctca cagccaatga cgctgtacca gtacgggtgt agacattaaa
     9961 ccggctggca ataatgattc cggagaatgc cggcggtaat gcgcctgcca gtaccatcat
    10021 ctgcagatgt tcgctgttca aatgacatgc cataccaacg agaagcagtg ccagtggcat
    10081 cagaatcagc ttcaggaagg tgttataagc aatttcggca ctgaactcga atttatgtgc
    10141 agccagagtc aaccccgcag cgaataccgc tacccctgag ttagccttcg caatcagatt
    10201 aaaggttggg tcccatgctg ccggaatttt taccccaacc aacaccagga tcgttgccag
    10261 aacaggtgcc catactactg gctcctttgc cgcagaaatt aatgcgctca gattactatt
    10321 cttcttacca tccgctcctg aggaaggatt cagcaaatac agaccaatag gaatagtaat
    10381 tgcgttaaca ataatagaaa taattgctac cactaaacct gttgatacgg aatcaccata
    10441 aataggatcg agaactgcaa accccaggaa tccaatggta ggtgaacccg caattaatgc
    10501 acatacagct gcttctgcat gggtacgttt aaaaaatttg tagcaaccaa accaggagaa
    10561 aaagaaacat ccgacaataa ccacaagtga tacaagggtc agacgagtgt ccgcaaaaat
    10621 catttccctg tttgcccggg taatagaaac aaatagagcc gcaggaagag cgtagtttaa
    10681 taccagttta ttaaatgcgc gggcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa
    10741 ataacccaac agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat
    10801 aatagtattt ccgataaggg tcccctcacc atcgcataat gagttggcaa gaggagacga
    10861 tgtcagtatt taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc
    10921 agttcctgct gcagggtcga tgaccacatt aataatggtc ggtttgcgcg actgaatacc
    10981 ggtggttaaa gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa cgccacgaaa
    11041 cgcatccatt aatttgtcat accttgcatg gtgcaacaga tccgttggtg atggtgcacc
    11101 agcgccactg agatcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat
    11161 cgtcaccggc aggttatatc gacaaatcgt ttcaatttcc atcccactga aaccaaaagc
    11221 actatcacct tcaatggcga caaccggaga accagaagtc acgctagcac cgatggcata
    11281 gcccataccg atgcccatga caccccaggt accacaatcc agacgacgac gtggtttata
    11341 catatcaata atatttcgtg cattatccag ggtatttgca ccttcattaa ctaaataaat
    11401 atcctggttc tcgcgcaata cgtcgcgcac agcacttaat gcattaaagt aatttaatgg
    11461 ttgagtatct gtacttaatt tttcatgcat tttttgtgca ttttgctgct tgtggatatt
    11521 taaaatatcg cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt ctgccagcat
    11581 accttgcata ctggatgcaa tatcaccaac gactggcaca gcaatggggc ggttgctgtc
    11641 aatttcctgc ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt
    11701 accgtgtgcc aataaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc
    11761 cagggcaaac gaacgcgcag ctgccgcaga aagtggatgc gtatcttcaa ggatcccttt
    11821 cgccatagac attggcagga atggaatctg agtactttca ataaattcac gaagctgttc
    11881 atcagcttgt gaatacgccg cgcctttgcc aaggataatt aatggccgtt cagcttttgc
    11941 taaaagcgaa attgcgctag tgactgactt cgggcatggc aataatgctg gcgacggatt
    12001 ttcaacttta acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt
    12061 tgctggcaaa tcaagataaa ctccgccagg gcgacccgat acagagaccc ggatagcgcg
    12121 tgccaatgca atgccaagat cctgcggctg attaacgcga aatgctgctt tggcatacgg
    12181 ttttgccgca ttcatttggt ccagctcttc ataatcacct tgctgtaggt cgacgatcgc
    12241 gcggtcgctg gagccgctaa tcatgatcat cggaaaacca tttaccgttg cgttggccaa
    12301 tgcggtcaaa ccattgagga atcctggcgc agaaactgtc aggcagatcc ccggtttttg
    12361 ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac tgctcatgac gaaaaccaat
    12421 ataacgaatg ccttccgcct gggcatggcg tgccatatcc gtcacaggaa tacctacaac
    12481 accataaata gtgtcaatat tattctgttt taatgcttca acgatgatat gcataccatc
    12541 tgtcatttga agttgatctg acatgattaa taacctcgaa atttttataa attgggagcc
    12601 agaagccccc gttaatatca gatggcgtgg ttttgcttca ttgcagcaat ttcatcgtcg
    12661 ctataaccca gctcctgcaa tacagcagcg gtatgttcac ctaatagcgg cgcagcttta
    12721 atatccggcg taaaggcaga gaatttcatt ggacaaccaa cggtcagata ttttccacgc
    12781 aacggttgtt ccacttcaac aacactgcca ctttggcgca aagagggatc aagtgaaatt
    12841 tctttcatac ttaaaaccgg tgcacaagga atatcaaact gagtcaaata ggccaccgct
    12901 tcatgtttat caatagtgac agtgtatttt tcgatttcag caaaaatatc gaaaatatgt
    12961 ggctgtcgtg catgggctgt actgtatgcc ggatcggtaa tccattctgg tttgccgatg
    13021 gctttacagg tgttttccca gttttgctcc tgaatagtga aataaatata ggcgttagga
    13081 tcggtttccc agcctttaca tttcaggatc cagccaggct gaccgccacc acctgcatta
    13141 ccaccgcggg gaactgcatc accaaatgta ccattcggat actgcgggta ttcttccaga
    13201 taacccaatt tatcgagacg ctgctggtca cgtaatttca cgcggcaaag gttcaatacg
    13261 gcatcctgca ttgacatggt gactcgttgc ccacgccccg ttttttcgcg atgcagcaaa
    13321 gcagcaagta aaccgatcag caaatgcatt ccggtgttgc tgtcacccaa cgctgcagcg
    13381 cttaccagcg gcggaccatc ccaaaaaccc gtagtggatg ccgcgccacc cgctgcctga
    13441 gcaacgtttt cataggcttt tacattcaca taaggcgaac actcatcaaa ccctttgatc
    13501 gaaccaaaaa tcagacgtgg attgatttct tgaatatgct cccaggtgaa gcccatgtga
    13561 tcaatggccc ctggatgaaa gttctcgact aagatatcag cttcgcggat cagcttttcc
    13621 attacctctt tgccttccgc tgttttggta tttaactcaa tagaacgttt gttactgtta
    13681 agcatggtga agtaaagcgc atcgatatca ggaatatctc gcagctggtg acgcgttacg
    13741 tcaccaacgc cgggacgttc aattttaata acgtcagcgc caaaccaggc cagcatttga
    13801 gtacaagatg ggccagattg cacaccggtg aaatcgagaa ctttaattcc ttgaagtgga
    13861 gttgacatag caatacctat tagttaatta aatatgtgtt aatttagcgt ttatttaagg
    13921 cgtaatatct tctataaata acccgcaaaa tatcgtcgta gctgcactcg atatttttaa
    13981 aatcctgtat caagtgctga aaatcatttc atcgccactt ccaattcctt tctgcatggt
    14041 aagtagtgag gacgaaaaga agaatatgat aaatattgat tcgtggtaat cagagagagt
    14101 cgcgacattt gtgatgttat tcttacaact gtaagaagcg ccggtgattt acaagtaagg
    14161 gtatgattac gctttttaaa gcgtaccata aaacataaca taataaatga tttgtgatga
    14221 cacgggatta ttgggattta ctttgaggaa gataaaatgc gtgtttatag aaccgcgagc
    14281 gccaaataat tttatgacca caacctgtcg tattgtggtc ataaaaaatt gtcggctgga
    14341 gggagttaaa tcctttacct caatatactg cctgtcagca ttagttcaca aacagtgctt
    14401 cgctgtcgac gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt
    14461 gctttttatc aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttaagcggca
    14521 tcagatattg gttttccatt acgccaacac cggccaggcg aagttcttcc attgcgccct
    14581 ttttatcccc tttcgccatt ttttcattgg caatttttat cgcggcttct ttttcgggag
    14641 ttgctacata actctcagaa attccgacag atgcattaat gacaatatat tggtcatcat
    14701 tgagattggt ctttttacct ggcttagcga actttgccca ttcggtactg tcatctgaca
    14761 gcaaagcgga agcttcattg gtcagttctt ttgccttttc tggatcgcca tgaaacagag
    14821 ccaggcgcgc tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg
    14881 ctgctgccgt tttctgggtt tgctgaactt gctgagtttg ctgttgtgcc gctaccggtg
    14941 cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa
    15001 ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc catcatattg
    15061 ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt atcttacagt
    15121 tgtagttatt atcgcaagaa gtattttagt cttacaattg tagt
//
LOCUS       DAZGXZ010000122        14544 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000122 DAZGXZ010000000
VERSION     DAZGXZ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.122"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(203..922)
                     /gene="dcuR"
                     /locus_tag="ACHKJF_003993"
     CDS             complement(203..922)
                     /gene="dcuR"
                     /locus_tag="ACHKJF_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP0951262.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(919..2550)
                     /gene="dcuS"
                     /locus_tag="ACHKJF_003994"
     CDS             complement(919..2550)
                     /gene="dcuS"
                     /locus_tag="ACHKJF_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP0951263.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2731..2961
                     /gene="yjdI"
                     /locus_tag="ACHKJF_003995"
     CDS             2731..2961
                     /gene="yjdI"
                     /locus_tag="ACHKJF_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP0951264.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2973..3245
                     /gene="yjdJ"
                     /locus_tag="ACHKJF_003996"
     CDS             2973..3245
                     /gene="yjdJ"
                     /locus_tag="ACHKJF_003996"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0951265.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3472..3768
                     /gene="ghoS"
                     /locus_tag="ACHKJF_003997"
     CDS             3472..3768
                     /gene="ghoS"
                     /locus_tag="ACHKJF_003997"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0951266.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3796..3969
                     /gene="ghoT"
                     /locus_tag="ACHKJF_003998"
     CDS             3796..3969
                     /gene="ghoT"
                     /locus_tag="ACHKJF_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP0951267.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4088..5605)
                     /gene="lysS"
                     /locus_tag="ACHKJF_003999"
     CDS             complement(4088..5605)
                     /gene="lysS"
                     /locus_tag="ACHKJF_003999"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0951268.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5842..7299)
                     /gene="dtpC"
                     /locus_tag="ACHKJF_004000"
     CDS             complement(5842..7299)
                     /gene="dtpC"
                     /locus_tag="ACHKJF_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP0951269.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMAVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7358..9505)
                     /gene="cadA"
                     /locus_tag="ACHKJF_004001"
     CDS             complement(7358..9505)
                     /gene="cadA"
                     /locus_tag="ACHKJF_004001"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP0951270.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9585..10919)
                     /gene="cadB"
                     /locus_tag="ACHKJF_004002"
     CDS             complement(9585..10919)
                     /gene="cadB"
                     /locus_tag="ACHKJF_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP0951271.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(11285..12823)
                     /gene="cadC"
                     /locus_tag="ACHKJF_004003"
     CDS             complement(11285..12823)
                     /gene="cadC"
                     /locus_tag="ACHKJF_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP0951272.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPDTDSPSHSLTIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSV
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQEMLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(13083..13241)
                     /locus_tag="ACHKJF_004004"
                     /pseudo
     CDS             complement(13083..13241)
                     /locus_tag="ACHKJF_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(13704..14030)
                     /locus_tag="ACHKJF_004005"
     CDS             complement(13704..14030)
                     /locus_tag="ACHKJF_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0951273.1"
                     /translation="MKLTVSPLAEHDLEAIGDWIAQDNPVRAISFTEELYQQCLLIAE
                     SPVLYRERPELGAGIRGCSYGRYLLLFRVLDTEVRIERIVHGSRDINRVLAEPDDKPS
                     DHFWQP"
     gene            complement(14027..14290)
                     /locus_tag="ACHKJF_004006"
     CDS             complement(14027..14290)
                     /locus_tag="ACHKJF_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0951274.1"
                     /translation="MPTSVSLSPYFETFIREQIESGRYNNTSEVIRAGLRALEEREQQ
                     IKLESLQSAVTAGINSGESKSAEEVFGRLTHKYKKMAEGEQPI"
     gene            complement(14362..>14544)
                     /locus_tag="ACHKJF_004007"
     CDS             complement(14362..>14544)
                     /locus_tag="ACHKJF_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0951275.1"
                     /translation="IQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIAR
                     HYPEALASTVNKSRKA"
BASE COUNT         3971 a         3325 c         3351 g         3897 t
ORIGIN      
        1 gggtgacata atagttaatt aacttttgtt agcgttttga aattaaaaac accgttcacc
       61 tgaagagata ttaattttta gcgatgatgg agggataatt atatttgatc tggcacaagt
      121 tttactgatg aaggatgtaa cttgtgccag gggtattttg cattacggta ataattatta
      181 cttacagata accagcgatc cgttattggc aatattgttt cagtagtgag tagtgttctg
      241 cctgaatacg gtaacggtaa accggacgcc ccgtgacgcc ataatggata ctggtgaaca
      301 agatgtggca gttgaccagc cagatgaggt atttacggca ggaaacacgc gagatgttaa
      361 cctcgttagc cagctcgtcg gttgaaaact catagtcctg atgcgcgtca atccactggc
      421 acagcgtgcg taacgtctgc ggcgttaagc cttttggcaa gcgacgagga tcctgttcgt
      481 tggagctgct gccgtggatt agctgatcaa gctcggcctg gtcataatac tgatgttttt
      541 ccagcgccat tttcttttgc cgccagccgg tgagcgcctc ttcaaagcgg gaagcctgga
      601 agggtttgat caggtaatcc acgacaccgt aatgcagcga atctttaatg gttgccgcat
      661 cggctgcgga ggagatgaca atcacatcac ttttgcaacg cgcgttatgc aggacaggca
      721 gtaaatcgag cccgttctct ttttgcatat agatatcgag caatatcagg tcgataggcg
      781 tatcgctatt gaagataatc tctttggctt tctccagcgt cgaggctgtt ccacagcatt
      841 gaaagcctgg gatttgtgct acgtatcggc gattcagctc cgcgaccatt gcgtcgtcat
      901 cgataattaa tacattgatc atctgttcga cctctccccg tcccagggta tctggacaaa
      961 aaattgtgtg aaaatcccgg gttccgattc cacggcgatg ctgccgccga gattttctac
     1021 ctgttgtttg acaagtgcta aaccgacgcc tcgctcgctt ccttttgtcg agacaccttt
     1081 gtcaaaaatg tgatcgattt tgtcgggtgc gatccccggt ccatcatcgt taacttcgca
     1141 gtgcagccag ccgtgacggt agtgcaatgt tacgctaatt tcgccaccgg gttccggccc
     1201 taatgcttcc agcgcgtttt ctatcagatt tcccaatgtg gtaatcagcg tcgcgacctg
     1261 gtcctcactg ccgctgtctg gcagctggct ttcactgttt aaaatcagcg tatggcctaa
     1321 atcggtcgcg cgattaatct tgctgattaa aaaaccagcg ataaccggag atttgatctt
     1381 acccagcaga gagccaatct cttcctgata gttattggct gttttgagaa tgtaatcttc
     1441 caactgctta taactcttca gatgcaataa tccgagaatc acatgcaatt tattcataaa
     1501 ttcgtgggat cgttcacgaa gtgcatcagc atagttgacc agaccgtcga gtcgctgcat
     1561 cagtttacga acttcagttt tgtccctgaa ggttgaaatg gcaccgatga taacgccatt
     1621 actgcgcacc ggaacggtgt tgatcagtaa tagccggtct ttaatcgtaa tctcttcgtc
     1681 gcggcgcggc gtaccgtcgc gtaacacttc cgagacatct accacctgtg accatgagtg
     1741 gctgagcgtc gacagtttct catcgtcctg cgacttacgg taattcagca attcttgtgc
     1801 ggcatcgttg atcagcgtga cctcgccgcg atcgtccacg gcaacgacgc cttctttgat
     1861 agactgcaac atggcctggc gctgctcaaa cagcgtggag atttcgtagg gttccaggcc
     1921 gaaaaggatt tttttcagta ccttaaccag aatgcaggtg ccaatcagtc cgaccagcat
     1981 gccaaataat accgaccaga taatgctcca gcgactgtca ttgatttgtt gggtcacgcg
     2041 gcttaactca aggccgatcg ccaccacgcc aatttgttta tgattttcat cgtagatggg
     2101 ggtaaatacg cgtaaagcct gcgccagaaa accgcgattg atagcgacat tttctttgcc
     2161 attcagcgct ttaaggatgt catcaccttt aaatggctga ccaatacgct gggcttcagg
     2221 atgcgagtag cgaagacttt gcatatcggt aacgacaata aacagcagat cgttgcgttt
     2281 gcgcacggct tccgcgatgg cctggattcc gctctcctgc ggttttttct gcaagccctg
     2341 acggatttcc ggcgagtcgg cgagggtacg cgccactgcc agtgccttgt tggccagccc
     2401 gtctcgcgtc atatcactga tttgcgagaa gtaaatcaga tgcaccacca atagcaccga
     2461 gaacagtacc gcgctgacca ttaagatcac tgtggtactc aatttcatcg gacgtttgcg
     2521 taacatgcgg tagggcaatg aatgtctcat cagcttcctt gtgtgacaaa tttcttaagc
     2581 attatctctg atgaggcggg taattcaaag ggagtaagaa tgattggcta tataggggaa
     2641 gagactctgg caacggaaac tgccagtgct gtatgaagat tccagggcta tgcttatagc
     2701 gataatcata ctgatgagag agggaaagtc atggatcagg cgctactgga cgagggttat
     2761 cgctgttata ccggcgaaaa gatcgatgtc tatttcaaca ctgcgatatg tcagcattct
     2821 ggcaattgcg tacgtggcaa cggcaagtta tttaatctca aacgaaagcc gtggatcatg
     2881 ccggatgaag tcgacgtcgt cactgtggtt aaagtgattg atacgtgccc gagcggcgcg
     2941 ctgaaatacc gtcataaata agcgagggta aaatggaaat acgcgaaggc cacaataaat
     3001 tttacattaa tgacgaacaa ggcaagcaaa tcgctgaaat tgtctttgtg ccgaccggag
     3061 agaatttagc gattatcgaa cataccgatg tcgatgaaag cctgaaaggg caggggattg
     3121 gtaaacagct ggttgcgaaa gtcgtggaaa aaatgcgtcg ggaaaaacga aaaattatcc
     3181 cactatgccc atttgcgaaa cacgaatttg ataaaacgcg ggagtatgat gatattcgca
     3241 gttgatggga gagtacagag tcacgatatt tttcatgctc tccgcggagt gatgcaggag
     3301 agcatctgaa gggtaggggg atgcacaaag aatgggcaga gagcgcgttt ttttgtccca
     3361 agtcatcccc tttactgagc aaaaaaaaga atatctccta tatgagaatc atcaatcggg
     3421 gttaataatt tttgcgtccc cagagcattc aatattgata ggagtcatat tatggaaggt
     3481 aaaaacaagt tcaatactta tgttgtttct tttgattatc catcatctta ttcctcagtg
     3541 ttcttaaggt taagatcatt gatgtatgat atgaatttct cttctatcgt ggctgatgaa
     3601 tatgggatac cacgacaatt gaatgaaaat tccttcgcaa taacgacatc gttagccgca
     3661 agtgaaatcg aagatttaat caggcttaaa tgcttagact taccggatat tgattttgac
     3721 ctcaacatta tgacagttga tgactatttc cgtcagtttt acaagtagcc aggaaggata
     3781 agaagaacat aaaaaatggc actattctct aaaatattaa ttttttatgt gattggtgtg
     3841 aacatatcct ttgtcattat ctggtttatc tcacatgaga aaacacatat tcgtttactt
     3901 agtgcattcc tggtcggaat aacctggcca atgagtctgc ctgtggcatt acttttttct
     3961 ctcttttagg agctgtagtt gagtggaccc gctggttatc gtgaaacgaa gcgtctctcc
     4021 tcaaaactgg atttgcggaa cgacgaacga agcagtcgcc tgactgcttc gttcattaaa
     4081 gtgaaattta tttctgtggg cgcatcgccg ggaagagaat aacgtcgcga atagtatgac
     4141 tgtttgtaaa cagcataatc attcggtcga taccaatacc cagaccagcg gttggcggca
     4201 gaccatattc cagcgcagtc acgtaatctt cgtcatagaa catggcttcg tcgtcacctg
     4261 cagctttagc gttaacctgt tcctggaaac gttcagcctg atcttctgcg tcgtttaatt
     4321 cgctaaaacc attaccgatt tcacgaccac cgatgaagaa ttcaaaacgg tcggtgattt
     4381 ctgggttaac atcattacgg cgtgccagcg gtgacacttc tgccggatat tccgtaataa
     4441 aggttggctg aatcagatgt gcttctgcca cttcatcaaa gatctccgtg acaatacgtc
     4501 ccagccccca gcttttctct accgtaatac cgatagattc agctaatgct ttagcagcat
     4561 caaaattatc caggtcggcc atatcggttt ctggacgata ttttttgatt gcttcgcgca
     4621 tggtgagttt ttcaaacggt ttgccgaaat caaacacatg ctcgccataa gtgactttag
     4681 tggtacccag aacctcttgt gccagggtgc ggaacaggga ctctgtcagt tcaatcaaat
     4741 cgtggtaatc cgcatacgcc atgtagagtt ccatcattgt gaactcagga ttatggcgaa
     4801 cagaaatacc ttcattacgg aagttacggt tgatttcgaa tacccgttca aaaccgccta
     4861 caaccagacg tttcagatac agctccggcg cgatacgcag gtacatatct aaatccagag
     4921 cgttatgatg ggtaataaac gggcgagcag atgccccacc tggaattacc tgcatcatcg
     4981 gggtttctac ttccataaag ccgcgcgcga ccatgaactg gcggatagcg gccagaattt
     5041 ttgaacggac aacaaacgtt tgacgggatt tatcgttagc gatgaggtcc agataacgtt
     5101 gacgataacg gacttcctga tcctgcagac catggaattt atctggtaaa ggacgtagtg
     5161 cttttgtcag caggcgcaac tcagtacagt gaatggaaag ctcaccggtt tgcgtcttaa
     5221 acagcgtacc gcgggcaccg ataatgtcac ccagatccca ttttttaaac tgatcgttat
     5281 aaacaccttc tggcaggcta tctcttgcaa cgtacagttg aatacggcca ccgacatcct
     5341 gcaacgttac aaaggaggct ttccccatga tacgacgggt catcattcgg ccagcaaccg
     5401 agacttcaat gtttaaggat tccagttcct ggttatcctt cgcatcaaac tcttcgtgca
     5461 gctggtcaga ggtatggtcg cggcgaaaat cattgggaaa cgccacacct tgctgacgta
     5521 gtgccgccag tttttcgcgg cgatttctca gttcatcgtt aaaatcaata gcctcattgg
     5581 ctccccgtgt ttcttgttca gacattttgg ttcctctaaa tccagctttc aatttagcgt
     5641 agataaagag acagatcggt acactgtatt atctgcctcg actattaata actcagttta
     5701 tcttatcgaa actaacgctt acctcaagtt gatatcacta ataaataacc atcaaaatgg
     5761 tttaaatgac ttattaatct agatttgcta gtgttataaa ctgaaagtta atttattcat
     5821 aaccaattgt ttttacgaca gttaatcgtt gctctcctgt atcatattcg tcggcgtgct
     5881 aaacagaaaa cgggtagcaa aggccaatac gacgatgatc gcgacacaag ccaacgtcca
     5941 ctctcccatc tgagaaaaga atcgctggta agctgcaatt gccataccgc taatttgcga
     6001 ctctgtcgtc tgctgtgcca cgacgcctgc cagccagttg gcgaccgcgc ctgttgccag
     6061 catataaata ccggttaata cgccagacat tttcagacgc gtgatttgcg caatcgccac
     6121 cgggtcaata aagagttcgg caaagcccat tagcgccagc ccggatatca tcacggccat
     6181 tgacgcttga ccgccagccg ctgcatgtcg ggcatcaaat gccaacaaca taaagccaca
     6241 agccatcagc agtaagccaa aggcaaactt cagccagacg cgcaatgttg agttgccgcg
     6301 gctttctggc gacgccagcc aggccagtac aaccccagcg agcatcactg caattgcatt
     6361 caccgactgg aatagtgctg taggtacttc aatattgaat gcctgacgat tcacaaagcg
     6421 atcgataaac aagctgatgg tactgccgcc ctgttgtgcc agtacccaga acaacgtccc
     6481 gacaaacatc aacaatacaa tttgccaaag agcacggcgg tgttcgggga atttgatcat
     6541 catgcgggca atgatttgtg cggcaatgag gcaaacgatc gccagcaaat atccggacca
     6601 gtcgttctcc agcagcaggg taaaaaatac tggggctaaa cagagcatca ccactaacca
     6661 gctccatact ggtaaggcaa atttcacgct agtgagcgct tttttatcca tactacgcgt
     6721 ggactggaaa tgacgatgcc cgcttaagaa aatcaacaaa ccgataaaca tgccgccacc
     6781 cgcaagggca aagccaacat gccatccata ccactgagca gccaggccgc aggcgattgg
     6841 ggcggcgata gaaccgatat tgcccgcagc atacagcagc gaaaaaccgc catcacgtcg
     6901 atgatcgttc tcgtcgtaga gctcgccaag caaacagctg atgtttgatt tgaataaacc
     6961 gtagccacaa ataatgattg ccagcgccag atacaggcta aaggttgaat ttgtatcaat
     7021 gcccagcacc acatggccaa gggtcattaa cagcgcgccg gcaatcactg cagtgcggtt
     7081 gccgagcagg cggtcggcaa gccagccgcc gagaataggg gtaacgtaaa ccagagaagc
     7141 atatgcgctg aacaggctga tggcatggtt atcatcaaaa ccaagctgat gggtgagata
     7201 gagaatgagt aaggcacgca tgccgtaaaa actgaagtac tcccagattt ggatcgccac
     7261 gatatagtat atcgcgcgcg gctgtgaggg tgttttcatg tgttctcctt atgagcaaaa
     7321 aagggaagtg gcaagccact tcccttgtac gagctaatta ttttttgctt tcttctttca
     7381 ataccttaac ggtatagcgg ccatcagcct gacggtatgc accgtgaata tcggtttcaa
     7441 agcccggata gtgagcgccg atttcacaca gcatctgcag gaactccaga accggacggc
     7501 tttcttcggt gatcatttca cccggcatta ccagaggaac tcccggcgga tacggaagga
     7561 tcatattggc gttaatacga cctaccattt cgtcgaggta aacttcttcg gtcataccgt
     7621 gcagctcttt ctggaatgca gcatacggag tcattaccat cgtcggcagc acttcaaatg
     7681 cgcggtacat cagatccggc agattgtggt gaacaatcag tttgtggata ttctgagcca
     7741 gttcctgaat acgcatgttt tcatagaatt caggatcttc acgatacaga gacggcagca
     7801 tgtttttcac acgcaggttc aggtcgaacg cacgtttgaa gtcagtcaga gcacgcagca
     7861 ggctcagtgc tttggtctta tcgataccga tgctgaacag gaacagcagg ttatacggac
     7921 cggttttctc aacaacgatg ccatgttcgt cgaggtattt cgccacgatg ctggccggaa
     7981 taccaaagtc gctcatggtg ccgtcttttt ccatccccgg agtcagcagg gtgactttga
     8041 tcgggtcaag atacatgtgc tcgttatcga tgtttttgaa gccgtgccag gtgctgtcag
     8101 aacgcagcgg ccagcattca gtggtatcga tatgatccgg ctgccataca tcaaagaacc
     8161 agccatcaga ttccgttctc agacgtttga tctctttacg gaatttgatc gcacgttcaa
     8221 tggaaccgtt gatcagacgc ttaccagcat tgcctttcat catcgccgct gcggtttcag
     8281 tggacgccac gataccgtag tgcggagaag tggtggtgtg catcatgtag gcttcgttaa
     8341 aggtttcttc gtttacgtca cctttaacgt ggatcatgga agcctgagag aacgccgcca
     8401 gcagtttgtg agtggactgg gtttcgtaaa tcactttccc ttctacacgg ccaccgctca
     8461 taccgcattt accttcgtaa atcggtgaga agttggtgta aggcacccac gcggagtcaa
     8521 agtggatgga tttcacatcc agtgttttct tgataaagtc ggtgttgtac agcagaccat
     8581 cataggtaga gttggtaatt acagcatgta ccggccaggt tgcgtttggt gtttctttca
     8641 cgcgcttagc aatggtagcg tgctggaatt cactctgtgg gataccacca agaataccgt
     8701 aagcgttacg ggtcgggcgg aaatagattg gcgtaacatc gctcatcatc atcaggtggg
     8761 tcagcgattt gtggcagtta cggtcaatca gaatggtgct gcctgctgga gcagagtaca
     8821 taccaacaat tttgttcgca gtggaagtac cgttggtcac catgtagctg cggtctgcgt
     8881 taaagacgcg agcgatatac tgttctgctt ctttgtgtgg accactgtga tccagcagag
     8941 aacccagttc agatactgaa atggaaatat cagatttcat ggtgttcgga ccaaagaaat
     9001 catagaacag gctacctacc gggcttttct ggaatgcagt accgcccatg tgaccaggag
     9061 tacagaaagt atatttacct tcacgaacat atttaaacag tgctttagtc agcggaggca
     9121 gaatagtgtt gatatattcg tcagtggtct gcttgatctt attagcaata tcttcagcag
     9181 cacccagcgc atattcaaag aagctaatct gtaaacgcag gtcattcagg cttacatcga
     9241 gagtggaata cgtattagcg aacgcgtaca acggcaggtt ctcgttcatt ttgctaattt
     9301 cttcgcacag ctcgagatta tatttatccc agtcaaaaat aacgccgcac agacgcgcat
     9361 tgttttcgat cagttttaat aagtcgtcac ggtcgttcgg gtaaacaatc tggaagttca
     9421 gacgttcaag cgcgcgatga agttcacgga tgggttcttc tttaaaataa acccccatgt
     9481 gattcaatat tgcaataacg ttcatagtca tatctccagg taaaaaaggc ccctcccaac
     9541 acatgggaca aaatgaaagg aggagcctcg gaaaatactt ttaattaatg tgcgttagac
     9601 gcggtgtggt tatccattga gtggctctgg cgctcgtgca ttttgcgagc gtagaacatc
     9661 aggataatca ggctgacgat gaaggtacct gccagctcga aggagcttgc gcccatcagc
     9721 gcgatgaagc agaacacgca acccagtaca gagcagatca ggctgacaaa gttgcggatg
     9781 ttaacgcctt caaaacgaat caggtcaacg caagagtaga agtacggcag catagtcagc
     9841 agtactgcga taccggtcag ttcaccgaac aggtcagatg ctttaccacc ggcagagttc
     9901 atcagagtga tgaggatcat cagggcagtc attttcactg cagccagcag cagacctttt
     9961 ttcggaatac cgttgctgtc gacttcacca taaactttcg ggaagttacc gtcgttagcg
    10021 gcacgtacac ctgcctggcc taccaacatc atccaggagc ccagagaagt caggcacgca
    10081 aaggcggtga atgcagaaac cagcggcgca gcccagttac cgaggatagt tgaagcactg
    10141 attgcaaacg gagcaccgga agccgccatt acagaagacg gatacatacc ggaaagcacc
    10201 tgagtcgcag cgatataaac aatacctgct aaaccagtac ccagcatggt tgccagcgga
    10261 acggtacgtt tcgggttttt aaccatacca gtacttactg ctgcggattc aacacccacg
    10321 aaggcccaca ggcagagcag aatactttta atgatcgcat gaccatcagt ggtatccgca
    10381 gtattccagt tagctgcata agttgccgca tcaaaccaat gccagccaac aatagcagtc
    10441 atcaccacag gaataagaac cagcaccaga ccaatagtgg ttaaacggct tacccaggta
    10501 ccgccgagca tatttacaaa ggtaaatacc cagacgatag caatacaggc gatacccgcc
    10561 ggaacaggat catttaatac tgggaagaag gtggaaagat aagatacagc ggtaatacca
    10621 atcgccaggt taccaatcca gttagcatgg taataaagaa cacctgtctg aaaaccaaat
    10681 gcaggggaaa tttctccggc ataagcaatt gggccacctt gttgcgggtt ttttgttgcc
    10741 agtcgggcat atacatacgc cagcgacatt gcaccaataa tagagataat ccaaccccag
    10801 atagcaatac caccgatact tgctaggttc gcaggtaata atgcaatacc gctccccatc
    10861 atattaccgg caacaacacc ggtacaggca aatagcccga tcttcttggc agaactcatg
    10921 ctcttctcct aatttcattt ttgaatttgg agtccgggtc atgatgtata actatttcct
    10981 gaccagacca aactggcgat aagattactc acgaaaaaag gattaatcct aaagattagg
    11041 tgaaataaca caaaagtttc tgtaagtgag aacttgaggt tttttattaa cacatcagga
    11101 tcgcaagttg atatcatgaa aagataaaca tttaatgttt acaatggatt gcgtgacatt
    11161 ctctggttaa atttatgtaa taaaaattat gcggcaaata aattgccgca acatattata
    11221 ccaacaggaa catacaaaaa ctcaacaaca aatattcccg agcataaatc aacccgggag
    11281 ttacttattc tgaagcgaga aatttgtcga gataaggtac aacataagga acagaagtct
    11341 ggaatatacc attttcaatc cagtaaaggg tgtttgcccc tgggcgtaaa ttaaaggcgg
    11401 tgaggtatgc atcagctgct tcccggttca tccccttcat ttcataaacc ttgccaagca
    11461 acacataatt tagccaggac atttcaagat caatgccagt atttatcgcc tggtaagact
    11521 catctgtttt accttttacc agggcactga ccgcttttat ttgatatata atggacaggt
    11581 tattcaattc cggcagtgta acaatgttat ctatttctgt attcagtgct gctaattgtt
    11641 tttcatctaa aggatgttga gaatggcgca cgatatcaac taatgctttt tctgctctcg
    11701 cgtaggtaaa ttcgggggat gattgaacaa tctcacctag taattcactg gcacggttca
    11761 atgatttatc atcgccatgc agtaaataat catgtgcctg ataaaaatta gttaataacg
    11821 caccacgatg aggcaaaatt tgctggagca tctcctgcat tcgttgtggc cacggttggt
    11881 ttaacgcttt tgataaactc tccagtaaat cattttgaat cgccagctga tttccattag
    11941 tgatgacata acgtttatcc agcatggttg aaccatctgc attgtctacc aattttaccg
    12001 acataaagca ttgttgagca cggtattggc gctgattaac aaacgcaata gataatgttt
    12061 taccggaact gctcggttca tcaatgttgt agttgatttt gtcatgcacc ataaaggtgg
    12121 agaaggtgtt aagtgatgtc gccaccaaat cacccacgcc tatcgcgtaa gagagctgat
    12181 acggggaact ccagctgtta caacttttat ttaccatatt aatgtcaata tcgcgtggat
    12241 tgagcaaaat acgcgatttg ctcataggaa gacgtgtatc aagagttgaa aacgctacca
    12301 gtgctacaca gatacctaac gacaacagga aaaaaaacca tacccaaaag gtagtgaatc
    12361 gtttgctttt aactggggat tgttcaggtg gcgttgcggt gttttgaatg gtaagactgt
    12421 gggagggaga atctgtgtca ggaaccgcct ctggtatagg gggaggcgaa gatagcatta
    12481 tttcctctcc ctcttcttcg ctgtaccaga taaccggcac cattaattta tagccgcgct
    12541 ttggtacagt agcgatatag acaggactat cttcatcatt atcttttaat gatttacgta
    12601 gttctgagat actctgcgtc acaacgtgat tggtgacaat acttctcttc cagacattat
    12661 cgataagttc atccctgcta agtacttcgc cactgtgttg agcaaagaaa accagaagat
    12721 cgattaatct cggctcaagg gtaagttgac gcccattgcg gctaatttgg tttatggacg
    12781 gagtaacaag ccattcgcca acgcgaacta caggttgttg cataatagaa actcattcga
    12841 aaagggaatg gtacaatgat aattgccaca acctattttt accatctata gatggcttta
    12901 tttacatatt attggtgaat gcaagacgtt atttttacca gccataaact tcttgatcac
    12961 atagtattac gaaagaattt tactgagaac cagaagtaat attccttacc atcaaaattc
    13021 atcatctttg ccaaagaaaa atgtgcagaa aataatccat ggaaaattgc ccggagcgct
    13081 tactatttta atggattgtt agtctttgca tgagtaagcg gactgataca tttctctttg
    13141 ttctcattca aaaatctcat cagtcggcgt tctgcccgat gttgtgcttt atggatgcgt
    13201 tacgctcctg atgacgtcat ttgacgttca acagcatcac ggggccgcac gacatttcac
    13261 gtcagtcagt gctatagctc aggaacaaat tttcccgaat tgggatatac ccgcaaaatg
    13321 ctggtgttgt ggggagaatc tggttgagtt cggtagaaat gatttttagg cagaacgctt
    13381 aaattgtggc gtccagaaac gaaaaacgga cctccgtgga ggtccgttta tatgaatttg
    13441 gtgcccggac tcggaatgaa actggttata taaccaattg ttttgtatcg attgttttgt
    13501 ctttgttgtt ttcgaggcca ccaatgaggc ccccatgtgc aattcgaatt cttttgacgc
    13561 tataatccct cttttttgtg ctttgctgta tcattttgtc tggttcgata tgctgaattg
    13621 cggaatcgaa gtaacaacaa aatacagaga tttataaaat acctggctac ctgtgttagg
    13681 gagcgttaac gcacagattt tgtttatggc tgccagaaat gatctgatgg tttgtcgtct
    13741 ggctctgcaa gtaccctgtt gatatcccgg gaaccatgaa cgatacgttc gatacgaact
    13801 tcagtatcca gtaccctgaa cagcagaagg taacgaccat acgaacaacc tctgatccca
    13861 gcccctaatt cgggtctttc tctgtagagt actggcgatt cggctatcaa cagacattgt
    13921 tggtaaagct cttcggtaaa actgattgca cggacaggat tatcctgagc gatccagtca
    13981 cctatagctt caagatcgtg ttctgctaat ggtgaaacag tcagtttcat attggctgtt
    14041 caccttctgc catctttttg tatttgtgcg tcagtcgccc gaatacctct tcagcacttt
    14101 tactttcacc gctgttgatg cctgcggtaa cggcgctctg gagtgattca agctttatct
    14161 gctgttcccg ttcttccaga gcacgaagtc cggcacgaat gacttcactt gtattgttat
    14221 accgaccact ttctatttgt tcgcggataa acgtttcaaa gtaaggactg agggatacac
    14281 tggtaggcat ggtgattttc tcgccgattg attgctatca attaataata gttaatattc
    14341 gcgaattgta aaatacctct tttatgcctt gcggcttttg ttcactgtgc ttgccagggc
    14401 ttccgggtag tgtctggcaa taatatcatt aagcctgtca accagttctg gtttcctgaa
    14461 cgtgatgtgt actgagcctt tcttaaagta tttaatactg aacatctcgt cttcatagtc
    14521 ctctttaccc tgaacagact ggat
//
LOCUS       DAZGXZ010000123        14386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000123 DAZGXZ010000000
VERSION     DAZGXZ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.123"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            294..509
                     /gene="essD"
                     /locus_tag="ACHKJF_004008"
     CDS             294..509
                     /gene="essD"
                     /locus_tag="ACHKJF_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP0951276.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            509..1006
                     /gene="rrrD"
                     /locus_tag="ACHKJF_004009"
     CDS             509..1006
                     /gene="rrrD"
                     /locus_tag="ACHKJF_004009"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0951277.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            1223..1405
                     /gene="rzoD"
                     /locus_tag="ACHKJF_004010"
     CDS             1223..1405
                     /gene="rzoD"
                     /locus_tag="ACHKJF_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP0951278.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1496..1789)
                     /gene="iss"
                     /locus_tag="ACHKJF_004011"
     CDS             complement(1496..1789)
                     /gene="iss"
                     /locus_tag="ACHKJF_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFP0951279.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(2149..2343)
                     /locus_tag="ACHKJF_004012"
     CDS             complement(2149..2343)
                     /locus_tag="ACHKJF_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0951280.1"
                     /translation="MSTKNRTRRTTTRNIHFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            2732..3277
                     /locus_tag="ACHKJF_004013"
     CDS             2732..3277
                     /locus_tag="ACHKJF_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP0951281.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3252..5177
                     /locus_tag="ACHKJF_004014"
     CDS             3252..5177
                     /locus_tag="ACHKJF_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0951282.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            5174..5380
                     /locus_tag="ACHKJF_004015"
     CDS             5174..5380
                     /locus_tag="ACHKJF_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP0951283.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            5377..6978
                     /locus_tag="ACHKJF_004016"
     CDS             5377..6978
                     /locus_tag="ACHKJF_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0951284.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6959..8278
                     /locus_tag="ACHKJF_004017"
     CDS             6959..8278
                     /locus_tag="ACHKJF_004017"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0951285.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            8288..8620
                     /locus_tag="ACHKJF_004018"
     CDS             8288..8620
                     /locus_tag="ACHKJF_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0951286.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8676..9701
                     /locus_tag="ACHKJF_004019"
     CDS             8676..9701
                     /locus_tag="ACHKJF_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0951287.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFEPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGEAVSYKGMYGDTAIVVYSGQYVEKDVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDA
                     FVSVQLA"
     gene            9741..10139
                     /locus_tag="ACHKJF_004020"
     CDS             9741..10139
                     /locus_tag="ACHKJF_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0951288.1"
                     /translation="MTKDELIARLKVLGEQLNRDVSLTGTKEELALRVAELEEELDDS
                     DDTSGQDTPVSPENALTGHENEVVSEPPDTVIQDTADLVTVVALVTLHTDALHATRDE
                     PVDFVLPGTEFRVSAGVAVEMTERGLARMQ"
     gene            10151..10504
                     /locus_tag="ACHKJF_004021"
     CDS             10151..10504
                     /locus_tag="ACHKJF_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0951289.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEDSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            10516..11094
                     /locus_tag="ACHKJF_004022"
     CDS             10516..11094
                     /locus_tag="ACHKJF_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951290.1"
                     /translation="MAIKGLEQAVENLSRISRTAVPGAAAMAINRVASSAISLSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARIVLSRRRRRKKGQ
                     RSALKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            11091..11486
                     /locus_tag="ACHKJF_004023"
     CDS             11091..11486
                     /locus_tag="ACHKJF_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0951291.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            11494..12234
                     /locus_tag="ACHKJF_004024"
     CDS             11494..12234
                     /locus_tag="ACHKJF_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951292.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASASVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSSNGEFAAVAEITVTAS"
     gene            12250..12672
                     /locus_tag="ACHKJF_004025"
     CDS             12250..12672
                     /locus_tag="ACHKJF_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0951293.1"
                     /translation="MFLKTESFEYNGVTVTLSELSALQRIEHLAWLKEQEKKAESSGN
                     LQVSVEDLIRGGAFLVAMSLWHNHPQKTKLPSMNEAITQIEQEVLTTWPTEAIAQAEN
                     VVLRLSGMSEFVVNNAPEQADDAGPTEPVSAGKCSTVS"
     gene            12654..13088
                     /locus_tag="ACHKJF_004026"
     CDS             12654..13088
                     /locus_tag="ACHKJF_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0951294.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPTSPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            13081..>14386
                     /locus_tag="ACHKJF_004027"
     CDS             13081..>14386
                     /locus_tag="ACHKJF_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP0951295.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGVDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWSLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQAD"
BASE COUNT         3364 a         3577 c         4296 g         3149 t
ORIGIN      
        1 tgttgaggtt ttcttatgta tttgtggtgg tgttttgaac actcggtagc attctcataa
       61 atatcattca gtggtttacg tacgtaaaaa attggttatg ctgttaagag tggttacttc
      121 gtcacacagc ttaaacccgc cgtcgagctg gtttttccat tttttgagtc tcgatattag
      181 ctgataactc aatacctgag ttattcactg actccgagtc tgttacgttt ctgctttttt
      241 gcgatacgtt gtattccctc aatttacacc cgctttgtct gcgaggtggg gttatgaaat
      301 ccatggataa gttaacaacg ggtgtcgcct atggcacctc agcaggtagt gccggttact
      361 ggtttttaca gctgctcgat aaagtcacgc cctcacagtg ggcagcaata ggtgtgctgg
      421 gtagcctggt atttggcctg ctgacgtacc tgacaaacct ttatttcaag attaaagaag
      481 ataagcgcaa ggctgcgaga ggtgaataat gcctccatca ttacgaaaag ccgttgctgc
      541 tgctattggt ggcggagcaa ttgctatagc atcagtgtta atcactggcc caagtggtaa
      601 cgatggtctg gaaggtgtca gctacatacc atacaaagat attgttggtg tatggactgt
      661 atgtcacggg catacaggaa aagacatcat gctcggtaaa acgtatacca aagcagaatg
      721 caaagccctc ctgaataaag accttgccac ggtcgccaga caaattaacc cgtacatcaa
      781 agtcgatata ccggaaacaa tgcgcggcgc tctttactca ttcgtttaca acgtgggtgc
      841 tggcaatttc agaacatcga cgcttcttcg caaaataaac cagggcgata tcaaaggcgc
      901 atgtgatcag ctacgtcgct ggacatatgc tggcggtaag caatggaaag gtctcatgac
      961 tcgtcgtgag attgagcgtg aaatctgttt gtggggtcag caatgaacag agtaaccgcg
     1021 attatctccg ctctggttat ctgcatcatc gtctgcctgt catgggctgt taatcattac
     1081 cgtgataacg ccattaccta caaagcccag cgcgacaaaa atgccagaga actgaagctg
     1141 gcgaacgcgg caattactga catgcagatg cgtcagcgtg atgttgctgc gctcgatgca
     1201 aaatacacga aggagttagc tgatgcgaaa gctgaaaatg atgctctgcg tgatgatgtt
     1261 gccgctggtc gtcgtcggtt gcacatcaaa gcagtctgtc agtcagtgcg tgaagccacc
     1321 accgcctccg gcgtggataa tgcagcctcc ccccgactgg cagacaccgc tgaacgggat
     1381 tatttcaccc tcagagagag gctgatcact atgcaaaaac aactggaagg aacccagaag
     1441 tatattaatg agcagtgcag atagagctgc ccatatcgat gggcaactca tgcaattatt
     1501 gtgagcaata cacacgcgct tccagcggag tataaatgcc taaagtaata aaaccgagaa
     1561 atccatttac gaatgtttgc tgggtttctg ttttaacaac attttctgcg ccgccacaaa
     1621 ttttggctgc atcaacagtt ttctcctgtc caattcccga aacgaagaaa tgatgggtga
     1681 tggtttcctt tggtgttact gctgtcggtt tgtttccaac agtaaacgtc tgttgagcac
     1741 atcctgtaat aagcattgcc agagcggcag aaaacaacat ttttttcatc ttattatcct
     1801 gcattgttaa aaacggcaga atcctatgtg acaacaatta aacgatagtt aaatggattg
     1861 atgaaaatta aaactatata ggtggatgct cagcctattg gaggaggggg gcactcagaa
     1921 tcctgtggaa tgaaataaac cgctctatct gtccattacc cttttagctg cgctgtatcg
     1981 tcgccgtatt cccgcattaa ccatgaccgt agcccgacgg ggaattcctt ctgcgtgagt
     2041 gtgcgggaat aatcaaaaac gatgcacacc gggttttact gtgctgacag acgcagggtt
     2101 accctcatag tcgcttttcc ggtgcgatgg tggaagaaac cgggatgttc atccatcatc
     2161 actttggatt gatgtatatg ctctcttttc tgacgttagt ctccgacggc aggcttcaat
     2221 gacccaggct gagaaattcc cagacccttt ttgctcaaga gcgatgttaa tttgttcaat
     2281 catttggtta ggaaagtgga tgttgcgggt tgttgttctg cgggttctgt tcttcgttga
     2341 catgaggttg ccccgtattc agtgtcgctg atttgtattg tctgaagttg tttttacgtt
     2401 aagttgatgc agatcaatta atacgatacc tgcgtcataa ttgattattt gacgtggttt
     2461 gatggcgtag atgcacgttg tgacatgtag atgataatta ttatcatttt gcgggtcctt
     2521 tccggcgatc cgacaggtta cggggcggcg acctcgcggg ttttcgctat ttatgaaaat
     2581 tttccggttt aaggcgtttc cgttcttctt cgtcataact taatgttttt atttaaaata
     2641 ccctctgaaa agaaaggaaa cgacaggtgc tgaaagcgag ctttttggcc tctgtcgttt
     2701 cctttctctg tttttgtccg tggaatgaac aatggaagtc aacaaaaagc agctggctga
     2761 cattttcggt gcgagtatcc gtaccattca gaactggcag gaacagggaa tgcccgttct
     2821 gcgaggcggt ggcaagggta atgaggtgct ttatgactct gccgccgtca taaaatggta
     2881 tgccgaaagg gatgctgaaa ttgagaacga aaagctacgc cgggaagttg aagaactgcg
     2941 gcaggctagc gagacagatc tccagccagg gactattgag tacgaacgcc atcgacttac
     3001 gcgtgcgcag gccgacgcac aggagctgaa aaatgccaga gactccgctg aagtggtgga
     3061 aaccgcattc tgtactttcg tgctgtcgcg gatcgcaggt gaaattgcca gtattctcga
     3121 cgggatcccc ctgtcggtgc agcggcgttt tccggaactg gaaaaccgac atgttgattt
     3181 cctgaaacgg gatatcatca aagccatgaa caaagcagcc gcgctggatg aactgatacc
     3241 ggggttgctg agtgaatata tcgaacagtc aggttaacag gctgcggcat tttgtccgcg
     3301 ccgggcttcg ctcactgttc aggccggagc cacagaccgc cgttgaatgg gcggatgcca
     3361 attactatct cccaaaagaa tccgcatacc aggaagggcg ctgggaaaca ctgccctttc
     3421 agcgggccat catgaatgcg atgggcagcg actacatccg tgaggtgaat gtggtgaagt
     3481 ctgcccgtgt cggttattcc aaaatgctgc tgggtgttta tgcctacttt atagagcata
     3541 agcagcgcaa cacccttatc tggttgccga cggatggtga tgccgagaac tttatgaaaa
     3601 cccacgttga gccgaccatc cgcgatattc cgtcgctgct ggcgctggct ccgtggtatg
     3661 gcaaaaagca ccgggataac acgctcacta tgaagcgttt ttccaatggt cgtggcttct
     3721 ggtgcctggg cggtaaagcg gcaaaaaact accgtgaaaa gtcggtggat gtggcgggtt
     3781 atgatgaact tgctgccttt gatgaggata ttgaacagga aggctctccg acgttccttg
     3841 gcgacaaacg tattgaaggc tcggtctggc caaagtccat ccgtggctcc acgcccaaag
     3901 tgagaggcac ctgccagatt gagcgtgcag ccagtgaatc cccgcatttt atgcgttttc
     3961 atgttgcctg cccgcactgc ggggaggagc agtatcttaa atttggcgac aaagagacgc
     4021 cgtttggcct caaatggacg ccggatgacc cctccagcgt gttttatctc tgcgagcata
     4081 atgcctgcgt catccgccag caggagctgg actttactga tgcccgttat atctgcgaaa
     4141 agaccgggat ctggacccgt gatggcattc tctggttttc gtcatccggt gaagagattg
     4201 agccgccgga cagtgtgacc tttcacatct ggacggcgta cagcccgttc accacctggg
     4261 tgcagattgt caaagactgg atgaagacga aaggggatac gggaaaacgt aaaaccttcg
     4321 tgaacaccac gctcggtgag acatgggaag cgaaaattgg cgaacgtccg gatgctgagg
     4381 tgatggcgga gcggaaagag cattattcag cgcccgttcc tgaccgtgtg gcttacctga
     4441 ccgccggtat cgactcccag ctggaccgct acgaaatgcg cgtatgggga tgggggccgg
     4501 gtgaggaaag ctggctgatt gaccggcaga ttattatggg ccgccacgac gatgaacaga
     4561 cgctgctgcg tgtggatgag gccatcaata aaacctatac ccgccggaat ggtgcagaaa
     4621 tgtcggtatc ccgtatctgc tgggatactg gcgggattga cccgaccatt gtgtatgaac
     4681 gctcgaaaaa gcatgggctg ttccgggtga tccccattaa aggggcatcc gtctacggaa
     4741 agccagtggc cagcatgcca cgtaagcgaa acaaaaacgg ggtttacctt accgaaatcg
     4801 gtacggatac cgcgaaagag cagatttata accgcttcac actgacgccg gaaggggatg
     4861 aaccgcttcc cggtgccgtt cacttcccga ataacccgga tatttttgat ctgaccgaag
     4921 cgcagcagct gactgctgaa gagcaggtcg aaaaatgggt ggatggcagg aaaaaaatac
     4981 tgtgggacag caaaaagcga cgcaatgagg cgctcgactg cttcgtttat gcgctggcgg
     5041 cgctgcgcat cagtatttcc cgctggcagc tggatctcag tgcactgctg gcgagcctgc
     5101 aggaagagga tggtgcagca accaacaaga aaacactggc agattacgcc cgtgccttat
     5161 ccggagagga tgaatgacgc gacaggaaga acttgccgct gcccgtgcgg cactgcatga
     5221 cctgatgaca ggaaaacggg tggcaacggt acagaaagac ggacggcgag tggagtttac
     5281 ggccacttcc gtgtctgacc tgaaaaaata cattgcggag ctggaagtgc agaccggcat
     5341 gacacagcga cgcaggggac ctgcaggatt ttatgtatga aaacgtccac cattcccacc
     5401 cttctggggc cggacggcat gacatcgctg cgtgaatatg ccggttatca cggcggtggc
     5461 agcggatttg gtgggcagtt gcgggcgtgg aacccaccga gtgaaagtgt ggatgcagcc
     5521 ctgctgccca actttacccg tggcaatgcc cgcgcagacg atctggtacg caataacggc
     5581 tatgccgcca acgccatcca gttgcatcag gatcatatcg tcgggtcttt tttccggctc
     5641 agtcatcgcc caagctggcg ctatctgggc atcggggagg aagaagcccg tgccttttcc
     5701 cgcgaggttg aagcggcatg gaaagagttt gccgaggatg actgctgctg cattgacgtt
     5761 gagcgaaaac gcacgtttac catgatgatt cgggaaggtg tggccatgca cgcctttaac
     5821 ggtgaactgt tcgttcaggc cacctgggat accagtccgt cgcggctttt ccggacacag
     5881 ttccggatgg tcagcccgaa gcgcatcagc aacccgaaca ataccggcga cagccggaac
     5941 tgccgtgccg gtgtgcagat taatgacagc ggtgcggcgc tgggatatta cgtcagcgag
     6001 gacgggtatc ctggctggat gccgcagaaa tggacatgga taccccgtga attacccggc
     6061 gggcgcgcct cgttcattca cgtttttgaa cccgtggagg acgggcagac ccgcggtgca
     6121 aatgtgtttt acagcgtaat ggagcagatg aagatgctcg acacgctgca gaacacgcag
     6181 ctgcagagcg ccattgtgaa ggcgatgtat gccgccacca ttgaaagtga gctggatacg
     6241 cagtcagcga tggattttat tctgggcgcg aacagtcagg agcagcggga aaggctgacg
     6301 ggctggattg gtgaaattgc cgcgtattac gccgcagcac cggtccggct gggaggcgca
     6361 aaagtgcctc acctgatgcc gggtgactca ctgaacctgc agacggctca ggacacggat
     6421 aatggctact ccgtttttga gcagtcactg ctgcggtata tcgctgccgg gctgggtgtc
     6481 tcgtatgagc agctttcccg gaattacgcc cagatgagct actccacggc acgggccagt
     6541 gcgaacgagt cgtgggcgta ctttatgggg cggcgaaaat tcgtcgcatc ccgtcaggcg
     6601 agccagatgt ttctgtgctg gctggaagag gccatcgttc gccgcgtggt gacgttacct
     6661 tcaaaagcgc gcttcagttt tcaggaagcc cgcagtgcct gggggaactg tgactggata
     6721 ggctccggtc gtatggccat cgatggtctg aaagaagtac aggaagcggt gatgctgata
     6781 gaagccggac tgagcaccta cgagaaagag tgcgcaaaac gcggcgacga ctatcaggaa
     6841 atttttgccc agcaggtccg tgaaacgatg gagcgccgcg cggctggtct taaaccgccc
     6901 gcctgggcgg ctgcggcatt tgaatccggg ctgcgacaat caacagagga ggagaagagt
     6961 gacagcagag ctgcgtaatc tcccgcatat tgccagcatg gcttttaatg agccgctgat
     7021 gcttgaaccc gcctatgcgc gggttttctt ttgtgcgctt gcaggccagc ttgggatcag
     7081 ccgcctgacg gatgcagtat ccggcgacag cctgactgcc ggagaggcac ccgcggcgct
     7141 ggcgttatcc ggtgatgatg acggaccacg acaggcccgc agttatcagg tcatgaacgg
     7201 catcgccgtg ctgccggtgt ccggtacgct ggtcagtcgg acgcgggcgc tgcagccgta
     7261 ttcgggaatg accggttaca acggcattat cgcccgtctg caacaggctg ccagtgaccc
     7321 gatggtggac ggcattctgc tcgatatgga tacgccaggc ggaatggtgg cgggggcatt
     7381 tgactgcgct gacatcatcg cccgtgtgcg tgacataaag ccggtatggg cgctggccaa
     7441 tgacatgaac tgcagcgcag gtcagctgct tgccagtgcc gcctcccggc gtctggtcac
     7501 gcagaccgcc cggacaggct ccatcggcgt catgatggct cacagtaatt acggcgctgc
     7561 gctggagaaa cagggcgtgg aaatcacgct gatttacagc ggcagccata aggtggatgg
     7621 caacccctac agccatctac cggatgatgt ccgggaaaca ctgcagtccc ggatggatgc
     7681 aacccgccgg atgtttgcac agaaggtgtc ggcatatacc ggcctgtctg tgcaggctgt
     7741 gctggatacc gaggctgcag tgtacagcgg tcaggaggcc attgatgccg gactggctga
     7801 tgaacttgtt aacagtaccg atgcgatcac cgtcatgcgt gatgcactgg atgcacgtaa
     7861 atcccgtctc tcaggagggc gaatgaccaa agagactcaa tcaacaactg tttcagccac
     7921 tgcttcgcag gctgacgtta ctggcgtggt gcaagcgacg gagggcgaga acgccagcgc
     7981 ggcgcagccg gacgtaaacg cgcagatcac cgcagcggtt gcggcagaaa acagtcgcat
     8041 tatggggatc ctcaactgtg aggaggctca cggacgtgaa gaacaggcac gcgtgctggc
     8101 cgaaaccccc ggtatgaccg tggaaacggc ccgccgcatt ctggcagctg caccacagag
     8161 tgcacaggcg cgcagtgaca ctgcgctgga tcgtctgatg cagggggcac cggcaccgct
     8221 ggctgcaggt aacccggcat ctgatgccgt taacgatttg ctgaacacac cagtgtaagg
     8281 gatgtttatg acgagcaaag aaacctttac ccattaccag ccgctgggca acagtgaccc
     8341 ggcacatacg gcaaccgcgc ccggcggatt gagtgcgaaa gcgcctgcaa tgaccccgct
     8401 gatgctggat acctccaccc gtaagctggt tgcgtgggat ggcaccaccg acggtgctgc
     8461 cgttggcatt cttgcggttg ctgctgacca gaccagcacc acgctgacgt tctacaagtc
     8521 cggcacgttc cgttatgagg atgtgctctg gccggaggct gccagcgacg agacgaaaaa
     8581 acggaccgcg tttgccggaa cggcaatcag catcgtttaa ctttaccctt catcactaaa
     8641 ggccgcctgt gcggcttttt ttacgggatt tttttatgtc gatgtacaca accgcccagc
     8701 tgctggcggc aaatgagcag aaatttaagt ttgatccgct gtttctgcgt ctctttttcc
     8761 gtgagagcta tcccttcacc acggagaaag tctatctctc acaaattccg ggactggtaa
     8821 acatggcgct gtacgtttcg ccgattgttt ccggtgaggt tatccgctcc cgtggcggct
     8881 ccacctctga gtttacgccg ggatatgtca agccgaagca tgaggtgaat ccgcagatga
     8941 ccctgcgtcg cctgccggat gaagatccac agaatctggc ggacccggct taccgccgcc
     9001 gtcgcatcat catgcagaac atgcgagacg aagagctggc cattgctcag gtcgaagaga
     9061 tgcaggccgt ttctgccgtg ctcaagggca aatacaccat gaccggtgaa gccttcgagc
     9121 cggttgaggt ggatatgggc cgcagtgcgg cgaataacat cacacagtcc ggcggcacgg
     9181 agtggagcaa gcgtgacaag tccacgtatg acccgaccga cgatatcgaa gcctacgcgc
     9241 tgaacgccag cggtgtggtg aatatcatcg tgtttgatcc gaaaggctgg gcgctgttcc
     9301 gttccttcaa agccgtcaag gagaagctgg atacccgtcg cggctctaat tccgagctgg
     9361 agacagcggt aaaagacctg ggcgaagcgg tgtcctataa ggggatgtat ggcgatacgg
     9421 cgatcgtcgt gtattccgga cagtacgtgg aaaaagacgt caaaaagaac ttcctgccgg
     9481 acaacacgat ggtgctgggg aacactcagg cacgcggtct gcgcacctat ggctgcattc
     9541 aggatgcgga cgcacagcgc gaaggcatta acgcctctgc ccgctacccg aaaaactggg
     9601 tgaccaccgg cgatccggcg cgtgagttca ccatgattca gtctgcaccg ctgatgctgc
     9661 tggctgaccc tgatgcgttc gtgtccgtac aactggcgta atcatggccc ttcggggcca
     9721 ttttctctgt ggaggagtcc atgacgaaag atgaactgat tgcccgtctt aaggtgctgg
     9781 gtgagcaact gaaccgtgat gtcagcctga cggggacgaa ggaagaactg gcgctccgtg
     9841 tggcagagct ggaagaggag cttgatgact cggatgacac ttccggccag gacacgcctg
     9901 tcagcccgga aaatgcgctg accgggcatg aaaatgaggt ggtatcagag ccgccggata
     9961 ccgtaattca ggatacggct gatctggtca cggtcgtggc actggtgacg ctgcataccg
    10021 atgcacttca cgccacacgg gatgagcctg tggattttgt gctgccggga acggagttcc
    10081 gtgtctctgc cggtgtggca gttgaaatga cagagcgcgg cctggccaga atgcaataac
    10141 gggaggcgct gtggctgatt tcgataacct gttcgatgct gccattgccc gcgccgatga
    10201 aacgatacgc gggtacatgg gaacgtcagc caccatgaca tccggtgagc agtccggcgc
    10261 agtaatacgt ggtgtttttg atgaccctga aaatatcagc tatgccggac agggcgtgcg
    10321 cgttgaagac tccagcccgt ccctgtttgt ccggactgat gatgtgcggc aactgcggcg
    10381 tggagacacg ctgaccatcg gtgaggaaaa cttctggata gaccgggttt cgccggatga
    10441 tggtggaagc tgtcatctct ggcttgggcg gggcgtaccg cctgccgtta accgtcgccg
    10501 ctgaaagggg gatgtatggc cataaaaggt cttgagcagg ccgttgaaaa cctcagccgt
    10561 atcagcagaa cggcggtgcc tggtgccgcc gcaatggcca ttaaccgcgt tgcttcatcc
    10621 gcgatatcgc tgtcggcgtc acaggttgcc cgtgagacaa aggtacgccg gaaactggta
    10681 aaggaaaggg ccaggctgaa aagggccacg gtcaaaaacc cgcaggccag aatcaaggtt
    10741 aaccgggggg atttgcccgt aataaagctg ggtaacgcgc ggattgtcct gtcccgacgc
    10801 aggcgtcgta aaaaggggca gcgttcagcc ctgaaaggtg gcggcagcgt gcttgtggtg
    10861 ggaaaccgtc gtattcccgg cgcgtttatt cagcaactga aaaatggccg gtggcatgtc
    10921 atgcagcgtg tggccgggaa aaaccgttac cccattgatg tggtgaaaat cccgatggcg
    10981 gtgccgctga ccacggcgtt taaacagaat attgagcgga tacggcgtga gcgtcttccg
    11041 aaagagctgg gctatgcgct gcagcatcaa ctgagaatgg taataaagcg atgaaacata
    11101 ctgaactccg agcagccgta ctggatgcac tggagaagca tgacaccggg gcgacgcttt
    11161 ttgatggtcg ccccgctgtt tttgatgagg cggattttcc ggcaattgcc gtttatctca
    11221 ccggcgctga atacacgggc gaagagctgg acagcgatac ctggcaggcg gagctgcata
    11281 ttgaagtttt cctgcctgct caggtgccgg attcagagct ggattcgtgg atggagtccc
    11341 ggatttatcc ggtgatgagc gatatcccgg cactgtcaga tttgatcacc agtatggtgg
    11401 ccagtggcta tgactaccgg cgcgacgatg atgcgggcct gtggagttca gccgatctga
    11461 cgtatgtcat tacctatgaa atgtgaggac gctatgcctg taccaaatcc aacaatgccg
    11521 gtgaaaggtg ccgggaccac actgtgggtt tataagggga acggtgaccc ttatgcgaac
    11581 ccgctttcag acgttgactg gtcgcgtctg gcaaaagtta aagacctgac acccggcgaa
    11641 ctgaccgctg agtcctatga cgacagctat ctcgatgatg aagatgcgga ctggactgcg
    11701 accgggcagg ggcagaaatc tgccggagat accagcttca cgctggcgtg gatgcccgga
    11761 gagcaggggc agcaggcgct gctggcgtgg tttaatgaag gtgatacccg tgcctataaa
    11821 atccgcttcc cgaacggcac ggtcgatgtg ttccgtggct gggtcagcag tatcggtaaa
    11881 gcggtgacgg cgaaggaagt gatcacccgc acggtgaagg tcaccaatgt gggacgcccg
    11941 tcgatggctg aagatcgcag tacggtgacg gcagcaaccg gcatgacggt aacgccagcc
    12001 agtgcttccg tagtgaaagg gcagagcacc acgctgaccg tggcattcca gccggaaggt
    12061 gtaaccgaca agagcttccg tgcggtgtct gcggataaaa caaaagccac cgtgtcggtc
    12121 agtggtatga ccatcaccgt gaacggcgtt gctgcaggca aggtcaacat tccggttgta
    12181 tccagtaatg gtgagtttgc tgcggttgca gaaatcaccg tcaccgccag ttaatccgga
    12241 gagtcagcga tgttcctgaa aaccgaatca tttgaatata acggcgtgac cgtcacgctt
    12301 tctgaactgt cagccctgca gcgtattgag catctcgcct ggttgaaaga gcaggaaaaa
    12361 aaggctgaat ccagtggcaa cctgcaggtg tctgtagagg atcttatcag aggcggggcg
    12421 tttctggtgg cgatgtctct gtggcataac catccgcaga agacaaagct gccgtccatg
    12481 aatgaagcca ttacgcagat tgagcaggaa gtgcttacca cctggcccac ggaggcaatt
    12541 gctcaggctg aaaacgtggt gttacgtctg tccggaatgt ctgagtttgt ggtgaataat
    12601 gcccctgaac aggcagatga cgccgggcct acagagcctg tttctgcggg aaagtgttcg
    12661 acggtgagct gagttttgcc ctgaaactgg cgcgtgagat ggggcgaccc gactggcgcg
    12721 ccatgcttgc cgggatgtca tccacggagt atgccgactg gcaccgcttt tacagtaccc
    12781 attattttca tgatgttctg ctggatatgc acttttccgg gctgacatac accgtgctca
    12841 gcctgttttt cagcgatccg gatatgcatc cgctggattt cagtctgctg aaccggcgcg
    12901 aggctgacga agagcctgaa gatgatgtgc tgatgcagaa agcggcaggg cttgccggag
    12961 gtgtccgctt tggcccggac gggaatgaag ttatccctac ttccccggat gtggcggaca
    13021 tgacggagga tgacgtaatg ctgatgacag tatcagaagg gatcgcagga ggagtccggt
    13081 atggctgaac cggtaggcga tctggtcgtt gatttgagtc tggatgcggc cagatttgac
    13141 gagcagatgg ccagagtcag gcgtcatttt tccggtacgg aaagtgatgc gaaaaaaaca
    13201 gcggcagtcg ttgaacagtc gctgagccga caggcgctgg ctgcacagaa agcggggatt
    13261 tccgtcgggc agtataaagc cgccatgcgt atgctgcctg cacagttcac cgacgtggcc
    13321 acgcagcttg caggcgggca aagtccgtgg ctgatcctgc tgcaacaggg tggtcaggtt
    13381 aaggactcct tcggcgggat gatccccatg ttcagggggc ttgccggtgc gatcaccctg
    13441 ccgatggtgg gggccacctc gctggcggtg gcgaccggtg cgctggcgta tgcctggtat
    13501 cagggcaact caaccctgtc cgatttcaac aaaacgctgg tcctttccgg caatcagtcg
    13561 gggctgacgg cagatcgtat gctggtcctg tccagagccg ggcaggcggc agggctgacg
    13621 tttaaccaga ccagcgagtc actcagcgca ctggttaagg cgggggtaag cggtgaggct
    13681 cagattgcat ccatcagcca gagtgtggcg cgtttctcct ctgcatccgg cgtggaggtg
    13741 gacaaggtcg ctgaagcctt cgggaagctg accacagacc cgacgtcagg gctgacagcg
    13801 atggcacgcc agttccataa cgtgacggcg gagcagattg cgtatgttgc tcagttgcag
    13861 cgttccggcg atgaagccgg ggcattgcag gcggcgaacg aggccgcgac gaaaggggtt
    13921 gatgaccaga cccgccgcct gaaagagaac atgggtacgc tggagacctg ggcagacagg
    13981 acagcgcggg cattcaaatc catgtgggat gcggtgctgg atattggtcg tcctgatacc
    14041 gcgcaggaga tgctgattaa ggcagaggct gcgtttaaga aagcggacga catctggagt
    14101 ctgcgcaagg atgattattt tgttaacgat gaagcgcggg cgcgttactg ggatgatcgt
    14161 gaaaaggccc gtcttgcgct tgaagccgcc agaaagaagg ctgaacagca gagtcaacag
    14221 gacaaaaatg cgcagcagca gagcgatact gaagcgtcac ggctgaaata taccgaagag
    14281 gcgcagaagg cttacgaacg gctgcagacg ccgctggaga aatataccgc ccgtcaggaa
    14341 gaactgaaca aggcactgaa ggacgggaaa attctgcagg cagatt
//
LOCUS       DAZGXZ010000124        14357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000124 DAZGXZ010000000
VERSION     DAZGXZ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.124"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            17..583
                     /locus_tag="ACHKJF_004028"
     CDS             17..583
                     /locus_tag="ACHKJF_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951296.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            634..3093
                     /gene="ybgQ"
                     /locus_tag="ACHKJF_004029"
     CDS             634..3093
                     /gene="ybgQ"
                     /locus_tag="ACHKJF_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0951297.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3108..3839
                     /gene="ybgP"
                     /locus_tag="ACHKJF_004030"
     CDS             3108..3839
                     /gene="ybgP"
                     /locus_tag="ACHKJF_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0951298.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3836..4897
                     /gene="ybgO"
                     /locus_tag="ACHKJF_004031"
     CDS             3836..4897
                     /gene="ybgO"
                     /locus_tag="ACHKJF_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951299.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5049..6095
                     /gene="abrB"
                     /locus_tag="ACHKJF_004032"
     CDS             5049..6095
                     /gene="abrB"
                     /locus_tag="ACHKJF_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0951300.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRFPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6092..6883)
                     /gene="nei"
                     /locus_tag="ACHKJF_004033"
     CDS             complement(6092..6883)
                     /gene="nei"
                     /locus_tag="ACHKJF_004033"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP0951301.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6919..7653)
                     /gene="pxpA"
                     /locus_tag="ACHKJF_004034"
     CDS             complement(6919..7653)
                     /gene="pxpA"
                     /locus_tag="ACHKJF_004034"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP0951302.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7643..8575)
                     /gene="pxpC"
                     /locus_tag="ACHKJF_004035"
     CDS             complement(7643..8575)
                     /gene="pxpC"
                     /locus_tag="ACHKJF_004035"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP0951303.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8569..9225)
                     /gene="pxpB"
                     /locus_tag="ACHKJF_004036"
     CDS             complement(8569..9225)
                     /gene="pxpB"
                     /locus_tag="ACHKJF_004036"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP0951304.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9248..9991)
                     /gene="ybgI"
                     /locus_tag="ACHKJF_004037"
     CDS             complement(9248..9991)
                     /gene="ybgI"
                     /locus_tag="ACHKJF_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP0951305.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10262..11743
                     /gene="dtpD"
                     /locus_tag="ACHKJF_004038"
     CDS             10262..11743
                     /gene="dtpD"
                     /locus_tag="ACHKJF_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP0951306.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11785..13203)
                     /gene="phrB"
                     /locus_tag="ACHKJF_004039"
     CDS             complement(11785..13203)
                     /gene="phrB"
                     /locus_tag="ACHKJF_004039"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP0951307.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13200..13709)
                     /gene="ybgA"
                     /locus_tag="ACHKJF_004040"
     CDS             complement(13200..13709)
                     /gene="ybgA"
                     /locus_tag="ACHKJF_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP0951308.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13851..14357)
                     /locus_tag="ACHKJF_004041"
                     /pseudo
     CDS             complement(13851..14357)
                     /locus_tag="ACHKJF_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3459 a         3839 c         3489 g         3570 t
ORIGIN      
        1 gttaaaggac tccgttatgt ttaaaggaca aaaaacattg gccgcactgg ccgtatctct
       61 gctgttcaca gcacctgttt atgcggctga tgaaggatcc ggtgaaattc actttaaagg
      121 tgaagttatt gaagcaccgt gtgaaataca tcaggatgat attgataaag aggttgaact
      181 cggtcaggtg accaccagcc acattaatca gtcacatcac agcgatgccg ttgctgtcga
      241 cctgcgctta gtcaactgtg atctggaaaa ctccagcaac ggttccggtg gcaagatttc
      301 taaagttgca gtcacctttg atagctcagc gaaaaccacc ggcgcagatc cgattctcaa
      361 caacaccagc acgggtgaag ccaccggcgt cggcgtacgt ttaatgaata aagatcaaag
      421 caatatcgtt ttaggcaccg ctactccaga tatcgacctg gctccgacct ccagcgaaca
      481 aacgctgaat ttctttgcct ggatggaaca aattgatcag gctacacctg taacgccagg
      541 cgcagttacg gcgaatgcaa cctacgtgct ggattataaa taagtattac tttggcggcg
      601 ctatcgccgc ctttcataaa atatttatca ggtatggaca ccgtgaatat ttatcgactc
      661 tcttttgtat cctgcctggt cgtggcgatg ccttgcgcat tggcggtcga attcaacctt
      721 aatgttctcg ataaatcaat gcgcgaccgc attgatattt cattattaaa ggaaaaagga
      781 gtcattgctc ccggtgagta ttttgttagc gttgcggtaa ataataacca aatcagtaac
      841 gggcaaaaga ttaactggca caaaaatgac gataaaacca ttccgtgcat caatgattta
      901 ctggtcgata aatttggctt aaaacctgaa gtccgtcagt cgttaccatt gataaatcag
      961 tgcgtcgatt ttagctcccg acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa
     1021 ctaaatatca ccattccgca agcctggctg gcgtggcact cagaaaactg gaccccaccc
     1081 tccacatgga aagaaggtgt cgccggtgtc ctgatggatt acaacttgtt tgccagcagc
     1141 taccgcccac aggatggcag cagcagcact aatcttaacg cctatggaac agccggaatc
     1201 aacacggggg catggcgctt acgcagtgat tatcagttga atcagactga tagcgatgat
     1261 aaccatgaac agtcaggcga aatatcacgt acctatcttt ttcgcccgtt accgcaatta
     1321 ggttcaaaat taaccctcgg cgaaacggat tttagttcca atattttcga cggtttttct
     1381 tataccggcg cggcactggc aagtgacgat cgaatgttgc catgggaact acgcggctac
     1441 gccccacaaa ttagcggtat tgcacagacc aatgccacgg tgacgatcag tcaatcaggc
     1501 cgcgtcattt accagaaaaa agtcccacca ggcccattta tcattgacga ccttaatcag
     1561 tctgttcagg gcacactgga tgtcaaagtg acggaagaag atggtcgggt gaacaatttc
     1621 caggtttcgg cagcatcgac gcccttcctg actcgtcagg gacaggttcg ctataaactg
     1681 gccgcgggtc agccacggcc ctccatgtca catcaaactg aaaatgaaac cttttttagc
     1741 aatgaagttt cctgggggat gctgtcaaac acctcgctgt acggcggcct gctgctttct
     1801 ggtgatgact accattctgc cgcaataggt atcgggcaaa atatgctgtg gcttggcgcg
     1861 ctgtcgtttg atgtcacgtg ggccagtagt cactttgata ctcagcagga cgagcggggc
     1921 ttaagctacc gttttaatta cagcaaacaa gtggatgcca ccaacagcac gatttccctc
     1981 gccgcttatc gtttctccga tcgtcatttt cacagctacg ccaactatct ggatcacaaa
     2041 tacaacgaca gcgatgcgca ggacgaaaaa cagacgatca gcttatctgt gggccaaccg
     2101 attaccccac taaacctcaa tctttacgcc aacctgctac atcaaacctg gtggaatgca
     2161 gacgcctcca cgaccgccaa catcacggca ggttttaatg ttgatattgg tgactggaga
     2221 gatatctcta tttcgacgtc attcaataca acccattacg aagataaaga tcgcgacaac
     2281 cagatttacc tgtcgatttc gctccccttc ggtaacggtg gtcgggttgg ctatgacatg
     2341 caaaacagta gccacagcac cacacaccgc atgtcgtgga acgatacgct ggatgaacgt
     2401 aatagctggg gcatgtctgc cggactgcaa tccgaccgtc cggacaatgg agcccaggtg
     2461 agcggtaact atcagcacct gagttcagcg ggtgagtggg atatttctgg tacctatgcc
     2521 gccaatgatt acagttccgt cagcagcagc tggagcggtt ctttcaccgc aacccaatat
     2581 ggtgcagcgt ttcatcgccg cagctccacc aatgaaccac gcctgatggt cagcaccgat
     2641 ggcgtggcag atattccggt tcagggcaat ctcgactaca ccaaccattt tggcattgcg
     2701 gtggtgccgt tgatttccag ttaccagcct tccaccgtgg cggtgaacat gaatgactta
     2761 cccgacggcg taacagttgc agaaaacgtt atcaaagaaa cgtggattga aggcgcgata
     2821 ggttacaaat cactggcttc ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc
     2881 agcggtcagt ttcctcccct cggagcggat atccgccagg atgacagcgg cattagcgtg
     2941 gggatggttg gcgaggaagg acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt
     3001 accgtggtct ggggtgatag ccagcattgc tcgctccatc ttcctgaaca tatggaagac
     3061 accgcaaatc gcctgatttt accttgtcat taattaagga aatgaaaatg acatttatga
     3121 aaggactacc ccttttgttg ttggttgcca gtctgtgcag ccacgctgca ctacaacccg
     3181 atcgcactcg tattgtgttt aacgctaatg ataaagctac cagtttgcgg gtggataatc
     3241 gtagcgataa actcccctac ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa
     3301 gcgatgatct tctggtcgcc ctaccgccga tacaacgtct ggaaccgaaa gcaacaacgc
     3361 aggtacggat agtaaagcaa gcctcgacca caaaattacc gggcgatcgc gaaacgctat
     3421 ttttctacaa tatgcgtgaa attcctcccg ccccggaaaa aaacagcgac catgccgttc
     3481 tgcaagtcgc cattcagagt cgtatcaagg tgttctggcg accggctgca ttacgcaaga
     3541 aagcaggaga aaaggtcgaa ctgcagttac aggtcagcca gcagggcaac caactgacac
     3601 tgaaaaatcc taccgcgtat tatctgacca ttgcctacct gggacgaaat gaaaaaggcg
     3661 ttctccccgg cttcaaaacc gtgatggttg cgccttttag tacggtcaac acaaacaccg
     3721 gaaattatag cggcagccag ttttatctcg gttatatgga tgattacggc gcgttacgca
     3781 tgacgacgct caactgcagc ggacaatgcc atttacaagc cgtggaggcg aagaaatgag
     3841 tgctggcaag ggagtgttgc tcgtcatctg tctgttattt ctgccgttga agtctgcgct
     3901 tgccctgaac tgctactttg gttcatccgg tggctcggta gaaaaatcag aagcgattca
     3961 accttttgct gtaccaggca atgccaaact cggcgataag atttgggaat ctgacgatat
     4021 taaaattccc gtctattgtg acaacaatac caacggcaat tttgaaagtg agcatgttta
     4081 tgcctgggta aacccctacc cgggggtgca ggatcgctat tatcaactcg gcgtaactta
     4141 caatggcgtt gattatgacg ccaaccaggg aaaaagccgc atcgacacta accagtgtat
     4201 tgacagtaag aacattgaca tttatacccc tgagcagatt atcgcgatgg gatggcagaa
     4261 caaaatttgc tcgggtgatc ccgctaatat tcacatgtca cgtacctttc tcgctcgcat
     4321 gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga
     4381 ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagatccta ctgcccaaaa
     4441 cctgaaatat catattaccg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt
     4501 ttccatttca ccggaaacac aggtgattga ttttggtaaa tttaatttgc tggatattcg
     4561 tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg
     4621 caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga
     4681 taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc
     4741 ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga
     4801 aaaaacctat acggctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt
     4861 cgatacagtg gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc
     4921 cggaaagggc atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat
     4981 tttttcctgg cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg
     5041 agcgaaggat gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta
     5101 ttggtttcct cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga
     5161 tcatcttcag tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc
     5221 aggccattct tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg
     5281 ccgccaactg gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg
     5341 tgggttggtt attggtgcgc tatagctcat tgcctggaaa taccggtgcc tggggttctt
     5401 ctcctggcgg cgcggcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc
     5461 tggttgcatt tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttttggtca
     5521 cccgcatgat gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc
     5581 cagtgagcat taatctcctg cttacgattt tgctcgcggt cgttgccggt acggcgggat
     5641 gcttgctacg ttttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc
     5701 agtctggtca gctcatcacc atcgaactcc cggaatggct gcttgcgatg gcgtatatgg
     5761 caattggctg gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc
     5821 cgctaccgca aatcctgctg tcgatttttg ctctgctggc tatttgtgcg ggtatggcgt
     5881 gggggctgac ccggtttatg cacattgatt ttatgactgc ctacctcgcc accagtcccg
     5941 gcgggcttga taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta
     6001 tggcgatgca aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt
     6061 ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa
     6121 acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc
     6181 catcacgatg aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct
     6241 gcccccgcgt agcgtaagaa aaccgaggaa tatccagtaa cgcgtgtgcg agtgcatcca
     6301 gttgcgccgc attgaggtct ttcgctttat gatttccagt caacccaacc tgccagagga
     6361 tctccacccg caaataattg ccaagtcctg ccagaaacgc ctgatcgagc agtaatccag
     6421 caaactgacg gttacgaaag cgcggtgaca ataaccgttc ctttaccacc tccggcgtca
     6481 gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt
     6541 ccggcctcaa catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt
     6601 gcagttttac ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc
     6661 agacgccgta aagctgatta tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca
     6721 acgccttacc acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag
     6781 tttttaactg cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct
     6841 ccaggttatc cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc
     6901 attctttatt aatcggggtt atgctgcgac aacaatctcc ttttcggcaa atgtagagcg
     6961 tagtcggcgg gcgaacgcca gtgcgtgctc gccgtcgcca tgcaggcaga ctgtttgcgc
     7021 cgtgaccgtt gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctctag
     7081 cgtttgcgcc agcgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac
     7141 cagcgagccg tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc
     7201 atattgcttg cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgctgg
     7261 atcgcaagcg tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg
     7321 gttgtacaac atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat
     7381 cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa acggtttctg gcggcagctg
     7441 catggcgctg cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac
     7501 accatttttt attgcttcac gcacgcaagc ctgcatggtt tgggcatcgc ctgcatgaaa
     7561 tccacaggca atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc
     7621 ttcgcccaga tcggcgttca ggtcaatttt cattgtgtag ccgccacgct aattgttcga
     7681 aataacgttg ttgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat
     7741 gaatcggctg accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg
     7801 caatacgcgg gtaaccaccg gtggtctgtg cgtcgttcat caacacaata ggctgcccgt
     7861 tatgcggcac ctgcaccaca cccggtaaca aaccgtgaga taacagttcg cgatcggtgg
     7921 tgcgttttaa aatttgcccc tgtaagcgat agcccatgcg gttactttgc gggctaagct
     7981 gccagggcga acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc
     8041 ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa
     8101 aatcacgctt cgatttgcca atcgggagtc ggtcaccatc cttcagtaaa cggccttcca
     8161 gcccgccaat ccccacgttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa
     8221 taccacccgc gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac
     8281 gctggcctgc tttcatcggc aatcgccagc cggtccagac ggcattatca tccagccgcg
     8341 cttcgcaacc ggcccctgtc agagcaaacc acccatcagt ttcgaactca accgttaact
     8401 gaccgagcgt gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa
     8461 tgcgcagcgc aggcatatcc agtgcgccgc agtggctgat acccgactgg cgaaaaccgt
     8521 gacgaccgcc atcctgcaca gtggtataca tgcccgcacg aataatcttc agcatactcc
     8581 ctccttctgc ggtacaaagc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg
     8641 cgccggatca aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc
     8701 cagcggataa acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa
     8761 gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa
     8821 gcccggttga aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac
     8881 ctgtttttcg ctcaacccgc aatgcgccgc gaccaccgcc aaatccggtc cgcctgcacc
     8941 accgtaaacc accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc
     9001 ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat
     9061 caccgtgata ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg
     9121 ctgcgccagt cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag
     9181 cactaccgcc gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga
     9241 ttattcgtta tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat
     9301 tcagccactc gctcaatgcg cgaataccac cacgttcagt ggcatggtga cctgcagcat
     9361 aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa
     9421 taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc
     9481 accaggcgac gcgcaggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc
     9541 ccagacgcgc ttcaatccag gaggctagtt ccagtcccgg cacaggcatg gtcagctctc
     9601 cccacggcac caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct
     9661 gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga
     9721 tatcattcgc cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact
     9781 ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct
     9841 catcgagcaa agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc
     9901 cttccacctg caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt
     9961 taatcagttg ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac
    10021 accgttaaca tagcgttacc tcgccagttc gtcgatagcc aatcttttga catattaaga
    10081 aacaaacccg acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata
    10141 tttatgcaat cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt
    10201 aaggaaacac aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat
    10261 tatgaataaa cacgcatcac agccgcgcgc tatttactat gtcgttgcgc tgcaaatctg
    10321 ggaatatttt agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact
    10381 aaaatacaac gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt
    10441 cacgccaatc ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat
    10501 gctgggggcg ttattgatgg cgatcggtca tgtggtgttg ggtgccagtg agatccatcc
    10561 gtcattcctc tatctgtccc tggcgataat cgtttgtggc tatggtctgt ttaaatccaa
    10621 tgtcagttgc ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt
    10681 ctcgctgatg tatgcagcgg gtaacgtggg gtctattatc gcacctattg cctgtggcta
    10741 cgcccaggaa gagtacagct gggcgatggg ctttggcctg gcggcggtgg gtatgatcgc
    10801 gggtctggtc attttcttat gtggcaatcg tcatttcaca catacccgcg gcgttaataa
    10861 aaaagtactg cgtgcgacaa actttctcct gcctaactgg ggatggctgc tggttctgct
    10921 ggtggcaacg cctgcgctga ttaccgtatt gttctggaaa gagtggtcgg tatacgcctt
    10981 aattgtcgcg actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa
    11041 ccagaaacag cgtaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt
    11101 ctgggccttc gcacaacagg gcggcagctc gattagcctt tatatcgacc gcttcgttaa
    11161 ccgcgatatg tttggttata ccgttccgac tgctatgttc cagtcgatta atgccttcgc
    11221 agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg
    11281 taccgtgcgc atctggggaa aatttgctct tggtctcggc ctgatgagcg ccgggttctg
    11341 cattctgacc ttaagcgccc gctggtcctc aatgtatggt cactcttctc tgccactgat
    11401 ggtattaggc ctggcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc
    11461 gcaaattacg cgcattgaaa tccccggtgt gaccggcgta ttaaccggca tttatatgct
    11521 gctttccggc gcgattgcga actatctcgc gggcgtgatt gccgatcaga catcgcaggc
    11581 ttcgtttgat gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga
    11641 tcaaattacc tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca
    11701 ggcgctgaaa ttcagaaacc gcgcgctggc gctggaatct taataggcca gcaccatacc
    11761 tgataaaacg ctcgctgcgt tttatcaggc tcctttcctc gcttcttcgt acgctgccag
    11821 cgttcgcagc cttgcctctt tgtgatcaac tatcggttgc ggataatcca gcatcacacc
    11881 tgctttctgc gcccacttcc acggctcatg caccgctttc cctggtacat cgcgcagctc
    11941 cggtaaccat cggcggataa actcgccctc acggtcaaat ttctcgccct gagttatcgg
    12001 attgaaaata cgaaaatacg gcgcagcatc agtacccgta gaagccgccc actgccagcc
    12061 accgttatta gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc
    12121 gcgccagtcg atcaacaaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt
    12181 atgcatccag ccagtactgt taagctgacg catagcggca tcaacaatcg ggtatcccgt
    12241 tttgcctttc tgccaggcct gtaaatgtgc gggattgctt tgccactgca cacgatccgt
    12301 ccaggcaata aacggacaat gtttacacaa cgaggggtaa tacgtcatca gatgacggta
    12361 gaactcgcgc cagatcagct cactaagcca gacactaccg gccccaccgt ccagcgcctg
    12421 cggctgttcc gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccag
    12481 gctggcggac aaacgactgg tgccttccac tgccggaaaa tctcgttgtt gctcatattc
    12541 tccggcaccg ttctggcaaa actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg
    12601 gaaatgcgca gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctc
    12661 tatcgatccg ctactacgaa cttttggcgc agcgacacac tccggcatcc cttcccgcag
    12721 ccgtttcagc caggcattct taaaaggcgt aaagactttg tacatctcgt gattaccggt
    12781 catcaccgcg ccaggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg
    12841 cagcgctctt tcaacttcca catcccgcgc ccgctcattc acttcatact gatagttata
    12901 aaacaggtgg gtaacgctgt tttccgcgca cacctgttta actatttcga cactggcgac
    12961 aaagtcatcc acttcacgga acaataaagg aatacctttt tccgcaagcg ctatttgtag
    13021 cccattcagt tgagcattga taagttcagc ctgacgcggc gacatgttat gcgtcgccca
    13081 ctggcgtggt gtagcgatat ataacgccag cacgcgtgca gacgaattgc ggcaggcggc
    13141 agccagtgcg agattatcgt gcagacgtaa atcctggcga aaccagacca gatgggtaat
    13201 cataaaactc ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc
    13261 cctgaaagcc aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca
    13321 agaagtggtt gctcgccacg gcgataactg tcgataagcg ccgccaactg ctggcgttct
    13381 gtggaatcaa tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg
    13441 cgcgggttgg cgggatgaga aagcaaaaca attacgcgtt ggtagtactg gttgtaaaaa
    13501 tcatccagat tttgccactg gtgaatatca gccacgaacg ggccaagttt gcggtactcc
    13561 ggctgagaat gcgccagaaa gacgagttta tagcggctgt gataatcgag aagcgccccg
    13621 cgcgttaagc catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg
    13681 gtgctgtcat caaatcgttg ctgattcatt actgctcctg aacggtttac aggagtaagt
    13741 gtagacgttg agaaatgata tttgtgaagt taatcctgtt gtagcaggac aaatttacga
    13801 aagcctgctc cccgcaagga ctgacgtcag gtagtttcta accatagctg ccctttatat
    13861 tcaaacgtga aatcaataag ctcatcaggg acatcgcaaa caatatgaag acgggtttct
    13921 tctctgccgt gactcttttc actaattgcg taactgtcat gcttgggatt atttaattct
    13981 ttcagcggaa atttttcctc gaaggcttta tttagccgcc cctggtttcc ttttacagcg
    14041 aataaataat cacctccctg tttttgtatc ttctctgcaa tatctttctg gcaacccatc
    14101 gcatcggttg tgatgatttt tcctttaata tccagcatgt taagaagttc agggatagct
    14161 gtgatctcat tggatttctt atccgtcttg atctgcccga tgaccagact gtgcattgtt
    14221 gagaacgcac taatgacatg aatcgctccc ctgcggcgac tcttgtcata agagtgccgg
    14281 agcgtttttc catcaattgc aatgacgtct ttatcatttg atgaatggca atcacgcatc
    14341 cagttaataa agcactc
//
LOCUS       DAZGXZ010000125        14010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000125 DAZGXZ010000000
VERSION     DAZGXZ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.125"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(463..999)
                     /gene="yfdR"
                     /locus_tag="ACHKJF_004042"
     CDS             complement(463..999)
                     /gene="yfdR"
                     /locus_tag="ACHKJF_004042"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0951309.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVMPLAPGHA
                     YGMFMERFNELSELRTYA"
     gene            complement(1128..1952)
                     /locus_tag="ACHKJF_004043"
     CDS             complement(1128..1952)
                     /locus_tag="ACHKJF_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP0951310.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(2018..2380)
                     /gene="yfdP"
                     /locus_tag="ACHKJF_004044"
     CDS             complement(2018..2380)
                     /gene="yfdP"
                     /locus_tag="ACHKJF_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP0951311.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(3160..3852)
                     /locus_tag="ACHKJF_004045"
     CDS             complement(3160..3852)
                     /locus_tag="ACHKJF_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0951312.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDFKSNTVCKNSTGEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYITDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            3950..4210
                     /locus_tag="ACHKJF_004046"
     CDS             3950..4210
                     /locus_tag="ACHKJF_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0951313.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNRNELKPQEKEVSRG"
     gene            4203..4754
                     /gene="ymfL"
                     /locus_tag="ACHKJF_004047"
     CDS             4203..4754
                     /gene="ymfL"
                     /locus_tag="ACHKJF_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP0951314.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     FNRLRADGDQIFPLGWAMILQRAAGTHYIADAVAQSAGGVFVSLPDIEEVENADINQR
                     LLEVIEQIGSYSKQIRSAIEDGVVEPHEQTAMNDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            4900..5079
                     /locus_tag="ACHKJF_004048"
     CDS             4900..5079
                     /locus_tag="ACHKJF_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0951315.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            5009..5899
                     /locus_tag="ACHKJF_004049"
     CDS             5009..5899
                     /locus_tag="ACHKJF_004049"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0951316.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIG
                     VLELHTATIGALASFLLLQLSYTQIMVGWVGAPKGAPVSVCAGYANPAQFTTSEIGVS
                     GGGSYPLHTEAAIMATIPALVQPELCIIADKVVTSSLAVASYFGKQHKNVIQKIASLE
                     CSAEFTELNFQLSEYIDASGRKLPCYQITRDGFAFLAMGFTGKRAAQFKEAYINAFNQ
                     MEKQLSKPPVPSDVAHNASVLYSYISSIHQVWLQQLYPMLAKAESPLAVSLYDYINDA
                     SALACLINLSLNPSEVRGRK"
     gene            5896..6120
                     /locus_tag="ACHKJF_004050"
     CDS             5896..6120
                     /locus_tag="ACHKJF_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951317.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            6117..6923
                     /locus_tag="ACHKJF_004051"
     CDS             6117..6923
                     /locus_tag="ACHKJF_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0951318.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVNEPIAVGASVDESVRVRSNRPEYSPEF
                     EQAWLVYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSGTQFV
                     KQAVTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            6920..7414
                     /locus_tag="ACHKJF_004052"
     CDS             6920..7414
                     /locus_tag="ACHKJF_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0951319.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPWVILCLTRQA
                     EELESRGLYRSAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAVSGDEWYLSGN
                     YVGA"
     gene            7414..8067
                     /locus_tag="ACHKJF_004053"
     CDS             7414..8067
                     /locus_tag="ACHKJF_004053"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0951320.1"
                     /translation="MSNKYCQELVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCTAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            8064..8390
                     /locus_tag="ACHKJF_004054"
     CDS             8064..8390
                     /locus_tag="ACHKJF_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP0951321.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTVVKDDDSEAVGIIRSLLAGEENARLRAAHWLH
                     ERGLKV"
     gene            8387..8782
                     /locus_tag="ACHKJF_004055"
     CDS             8387..8782
                     /locus_tag="ACHKJF_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0951322.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            8945..9760
                     /locus_tag="ACHKJF_004056"
     CDS             8945..9760
                     /locus_tag="ACHKJF_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP0951323.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     PTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            9768..10757
                     /locus_tag="ACHKJF_004057"
     CDS             9768..10757
                     /locus_tag="ACHKJF_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0951324.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPNLVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            10771..11523
                     /locus_tag="ACHKJF_004058"
     CDS             10771..11523
                     /locus_tag="ACHKJF_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0951325.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAESKARCERCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGAPVYKICGRCNGNRFSRLPTTLARHRVQKLIPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEEQLRKVTR"
     gene            11672..11748
                     /locus_tag="ACHKJF_004059"
     tRNA            11672..11748
                     /locus_tag="ACHKJF_004059"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11706..11708,aa:Arg,seq:tcg)
     gene            11762..11838
                     /locus_tag="ACHKJF_004060"
     tRNA            11762..11838
                     /locus_tag="ACHKJF_004060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11796..11798,aa:Arg,seq:tct)
     gene            11930..12889
                     /gene="stxA2b"
                     /locus_tag="ACHKJF_004061"
     CDS             11930..12889
                     /gene="stxA2b"
                     /locus_tag="ACHKJF_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0951326.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
     gene            12902..13165
                     /gene="stxB2b"
                     /locus_tag="ACHKJF_004062"
     CDS             12902..13165
                     /gene="stxB2b"
                     /locus_tag="ACHKJF_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0951327.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
     gene            13684..>14010
                     /locus_tag="ACHKJF_004063"
     CDS             13684..>14010
                     /locus_tag="ACHKJF_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0951328.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVLVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPD"
BASE COUNT         3523 a         3220 c         3614 g         3653 t
ORIGIN      
        1 tgcatggggt attataatta ttaatatttc gcctgatacc aactcaaaat cttttttgtc
       61 gcatccattg tggattaata ttcagaggtg aggggtgtct gcggaattta tattttgcag
      121 gatgagcata caatacatga tataacatct gtttttggca cagagcatat agtcagatta
      181 tgtttagttc tgtgccatag atggatacta tctcacctct tggctaactc tcattactca
      241 ataactccag caaatctgta tattttgcgt gatgcccatt tatttgggca ggatttaata
      301 tcaggatctg gaaaatctgg cctgtatttc tggcctgtcc tcctgtttac gctgttccag
      361 cgaagcaccg tcgatattga aacgccacag aattcggcga cttgttttgt tgtcattaag
      421 ttgctcattg ctacccctcc agcggttgtt cagttgaaag actcatgcgt atgtgcgtaa
      481 ctccgataac tcgttaaagc gttccataaa catcccgtag gcatggcccg gtgccagtgg
      541 catcacgttg aacatctctg ttgccgggat accttccagt actggccaga aagagccatc
      601 atcaagcccg agatcgcggc gttcggttgc cagcatgatg agatcggcat atttcacagg
      661 cgtgctcata accgggggta acccgtattt ctcacggatt acggcgtcta ttttttcttc
      721 catccgttta tagtcaggaa gaaggcgttt cagtggagcg ggaatatcct ggcaatacgc
      781 ttctgttgca tcatgcatta acgcttcaaa agcaaattcc tgcggcacca gctggctgca
      841 aagcaccgca tgctgggcga cactgtagaa gtgtgaaaga tgtcctgcaa agcgacagat
      901 atttgaaagg gaaactgcga tatcgttaat cacgatgtcg tctttattta tcctgtcata
      961 ataaaaatgc ttcccggaaa aagttttaat aaatgacatt tcgttctcca ctttatatgc
     1021 gctgcaccgc gttgaattct gctaaaagga agctctcacc atccggtgat tattgagtta
     1081 attacgtttc cataaatgcc cccgcagggg catttgcagt aatgaaatca ggcggtgaaa
     1141 gtaccaataa aggtttctac tttgctgtct ttgaatttct caacaagcag atcacgaaat
     1201 tcgttagcca tttcttcctg caccgcttcc agctgaataa tgcgcagaac cagtacagga
     1261 cgatcgccag tgataatgct gaggcgtaat ttaaacggac gttctttcag gccttcaaac
     1321 ggaacgcatt taaattcaaa tgccactggc ataatgtctt tggttttcgc ttcgacagac
     1381 tccatcagag agcgtttgcc gctgaagtca ttgtcttcaa aatcagcggt ctggtttgct
     1441 tcaattgtga ttttacggac tgccgcagcc gctttggttg cctgaatggt gtcaccatta
     1501 gcatcaaagc ccacaaggta gtcggcccag tcttcaatcc attctgccag tgacttctgg
     1561 gagttacgct cgccattaac agacagcagg gcagagaacg gtgctgtctt tttcagtttg
     1621 agagtggcgg tgttatctgc gtgacctggt tcatcaatag tacctaggtt aagcacactg
     1681 acggctcgca tattatcggc atcgataaag cagcgggtgc cttcatctgc aagatcttta
     1741 gaataacggg taaagtcatc gatgctggca gtggaaagcg caccacggaa acggaagcga
     1801 tttaaattaa atttttccag atcatgaatg cggaaattct caggcaatgc cacagcatcg
     1861 gcaccaatct tactgataat ttcattaaca ccctgagcag aaataagggc atggatttga
     1921 ttaattgcgg ttgcgtctaa gttctgagac ataataagtc ctcactatat taagatattc
     1981 agtgatgaga taaataatca gttaattaag aacgatatta atgacctgct gcgcggagtt
     2041 ttccgtcagg ctcaccggca agagtcagta attgtccctg gtcttcctgc agaatagtca
     2101 ggcgaccacc gcgattgaca tacatcggcg tttcggtggt gtcttcttcg gaaattttcc
     2161 cgcggttagt cgggcgaaca tatgagagtt tgtgtttgat tttcacacgg ttctcatcaa
     2221 acggttcgat ttccaggttg agcgagacct tacctttggt tttcgtgttc atcacaccgg
     2281 aagcgacttc actgagaact gcgccgattt ttgtttcaaa tacgccgccg tccagctccc
     2341 cgataaatgc ctgcacatca gtactgcgtt cgctagccat tttgttgctc ctcatcatat
     2401 cgaccctgca aggtcggttg gtttctccac aaaacagaga agaacacctg cggtggcagc
     2461 cgcccggatg gattgggtta tgagcccgtc gtccggtgat gctcttctct gttttgtaaa
     2521 aagagcggta ccagccggaa gcaagtgtac aaactggtac tgccaaagca gtggctgttg
     2581 tggtgaccgg tgctgatctc cggcttgcgg ttatttcaga ctctcacggg cgtttgattg
     2641 ccccgccgaa cagctctttt ccgcaatagc tgcaatgtct ttcgcgcatc agcctgcgca
     2701 ttcaccacaa cgctgagagc attgccggtg tccgaatcga acggaccttt tccctgccca
     2761 accctcccat ctgaatggga ctgtctggaa ttgaaccagc acttatgcct tgctcgtcaa
     2821 tgctctcatc gttgcgtcct ggtctcttcc caggcgtcaa accgaatcgc cacgctggtt
     2881 aggcgtctta tcagcatcct cattgacttg cacattccgg ctacctggtt tgtttgcccg
     2941 agcaaggagt ggattgtccc ctttaacgtc cccagaccgc taacgacgca tgtgccatac
     3001 gccgtgttac aaccaaattt tgttaggacc ttgtttgttg gtctggaaag aaagataaaa
     3061 tgaaattgcg cattatgcaa gtgtttttgt tgcgagatgc gcaatatgat gggtgatgaa
     3121 aagccacctt ctggtggcta attgatgagg aggtcggggt taattgtgtc gcttaagggt
     3181 ttgtgactga ctgattaaga ccttcccaaa gaccataaac cggtgttcgt tttcgctggt
     3241 aattccccat tcacggtaaa tctggttatc agaaatcacc agtagtttgt caggtatcat
     3301 ttgcagtcgt ttgacataaa ttttatcatc aaaaccaaac acatagatac catccccatc
     3361 aaactgattg atactgatat caacgaagat gagatctcct ggctcaatgg ttggacacat
     3421 actgtcccca cgaacgttga taactttaat gtgattggct ggtcgtcctc caaacatcga
     3481 tacagcatta tcagttatgt attcaatggc atgaatcaca tcaatgacat caccgccctg
     3541 gataaggcca tttcccgcac tggcactgac atccagcatt tcaatacgga atacatcctt
     3601 cacctgcgca acatcctcac cagtactgtt tttacataca gtattacttt tgaagtctga
     3661 ggtaaagaga tctgcaatat caacacctaa gctcctggca atattactca gggcttgttc
     3721 agtgaattgt ttctgcttac ctgtttccag gcgtgagata ttcgccgcat ccactcctat
     3781 tgcttcagcg agatcggcga ttttcatgtt cttcgcctgg cgaagttgtc tgactcgatt
     3841 tcctatgttc atgcgtttat tacatttctt tattgcgcgt taagcaaatc aacttgcgca
     3901 aaatatttgc gtgaaataat atgcttatca cgcaatatgt ggaggttata tgcaatcacc
     3961 attacgaaat gtgcgtaagg cgcacggatt tactttgcag catgttgctg cgggtgttca
     4021 ggttaatcca gcgacgctga gtcgtattga gagactggaa caaattccat ctatcgatct
     4081 ggcagagcgt ctggccaatt tttttaaggg agaaatcagc gaaatgcaga ttctctatcc
     4141 tgctcgcttt caatctagtc aaaaccggaa tgagttaaaa ccacaggaaa aggaggtaag
     4201 ccgtgggtaa gcatcactgg aaagtggaaa aacagcctga gtggtacgtg aaagcggtca
     4261 gaaaaactat cgcggcgttg ccgggtggtt acgctgaagc agctgagtgg ctggatgtaa
     4321 cagagaacgc tttattcaat cgccttcgtg ccgatggcga tcagattttc ccgctgggat
     4381 gggcaatgat tttacagcgc gcggctggca ctcactacat tgcggatgct gtcgcacagt
     4441 ctgctggtgg ggtgtttgta tcgcttcctg acattgagga agtagagaac gccgatataa
     4501 accagcgcct gctggaagtc atcgaacaga tcgggagtta ctcaaagcag attcgttcgg
     4561 caatcgaaga tggggtagtg gagccacacg agcagacagc aatgaatgat gagttgtatc
     4621 tgtcaatttc gaagctccag gagcatgcgg cactggtcta caaaattttc tgcgctccag
     4681 aaaagagtga cgcccgcgag tgtgcagctc cgggcgtcgt ggcgttttgt gtctgtggag
     4741 aaactaacgc atgaacagtt taacggcaaa taaccgtttg tcgcaacagc tggtggtcag
     4801 tgtcgctgca cacctgttgt tacggcatga atgcagatta ccaaatcacc tggctgtaag
     4861 taaccacaga gaactttacc tgactgtggg gggcgagttg tgcaggaact taaccgctgg
     4921 tttcgtgacg gaagagggct ttatgttcat gttattcgtt gggaaccaga aacacagcgc
     4981 gttatctatc ttcgcaaaga ctacccgcat gagtgcttta gtcctttgtg gaaattcagg
     5041 cgtgattttg ttgagtgtga aggaccacca gcacattgat tctgccattc cgggacgtta
     5101 cactgttcag gcaccttata aagcgggtgc cgggattggc gtcctggaat tgcatacggc
     5161 gacaattggc gcgttagcgt cttttttgtt gctacaactc agctataccc aaattatggt
     5221 gggctgggtg ggggcaccga aaggtgcgcc ggtttccgta tgcgccggtt acgccaaccc
     5281 tgctcagttc accaccagcg aaattggcgt ttccggtggt ggaagttatc cattgcatac
     5341 ggaggctgcc atcatggcta cgatccctgc cttagtacaa cctgaacttt gcattattgc
     5401 agacaaagtt gttacttctt ctctggctgt tgctagttat ttcggcaaac aacacaaaaa
     5461 tgtcattcaa aaaattgcgt ctcttgaatg ctctgccgaa tttactgagc tgaattttca
     5521 gctcagtgag tacatcgacg catcaggccg caaactacct tgctatcaaa taacccgcga
     5581 cggctttgcg tttcttgcta tgggtttcac gggtaaacgt gctgcccagt tcaaagaggc
     5641 atatatcaat gcctttaacc agatggagaa acagctttca aaaccccctg taccgagcga
     5701 cgttgcacat aacgccagcg ttctctattc ctacatttca tcaattcatc aggtctggct
     5761 gcagcagctt tatcctatgt tggcaaaagc cgaatctccg ctggctgtta gcttgtatga
     5821 ctatattaat gatgcttcgg cgctggcctg cctcataaat ttgtcgctga acccttcaga
     5881 ggtaaggggg cgcaaatgat ccggaatatt ttcaaacggt ttaccaatca gactttccgt
     5941 tgtcctcgtc cgggtcagtg gtataccaca cctgcagggc atgttctacg tgttagcctg
     6001 gttgaccgtg aatgtcagaa ggtgatttgt gaaccgctgg gccgtaatta ccgcgtcagt
     6061 atgccgctta tagcctttcg ctccggaaaa aacatgaagc atctcggagg tgcagcatga
     6121 gtatggagct gatggttaaa gcgatgaaaa ttcgagtggg taatccattg cgaaaactgg
     6181 ttctgatcaa gctggctgat aatgccagcg atcagggtga gtgctggccc agctaccagc
     6241 atattgctga ccagtgcgag attagcaaac gttctgtgat gaatcatatt gcggcccttt
     6301 gtgagtccgg gctggtaaaa aaagtcaccc ggaaaggtga aaaaggtaac tcaagtaata
     6361 tctatctcct tcatcttgat ggtgcaggag attcactagg gggtagtgca aataattcac
     6421 tatctggtgc agcaaattca ccaggtagtg caggagttgc accagggggt agtgcaggag
     6481 attcacccag aaccagtcac tcttttgaac cagtcaatga accaatagct gttggtgcat
     6541 cagttgatga gtccgtgcga gttcgttcaa accgaccgga atactctccg gagtttgagc
     6601 aggcatggct ggtatatccc aaacgtgctg gtggcaattc aaaatcagca gccttcaaag
     6661 cctggaaagc ccgtttgaac gagggggtaa accccgaaac catgctggaa ggtgtgaaac
     6721 gctacgcggg ctgggtatct gcgatgggca atagcggcac acaatttgtg aaacaggctg
     6781 tcacgttctt tggtccggat cgtcatttcg aagaatcctg ggaagttcct gcggtatctg
     6841 cagccagacg cgaggacccg tacttcaaag ccagttacga caacgtggac tacagccaga
     6901 tcccggcagg attcaggggg tgatcatgag tcttttgaat gacgttcaga aattcattga
     6961 agcccatccg gggtgtactt ccggagacat tgcggatgct tttgcaggtt actcacggca
     7021 gcgcgttctg cagtcagcaa gcaagttacg tcagagcggg cgtgtggctc accgttgtga
     7081 aggagataca cgcagacatt tcccgcgcct gactgagaga gcgcaggaac cggaaccaca
     7141 accagttcgt gaaaccagac ctgtgcgcaa tttctatgtc ggcactaacg atccctgggt
     7201 gattttgtgc ctgacccgcc aggctgaaga actggagtcc aggggcttat accgtagtgc
     7261 tgcaaccgtg tggatggcgg cattccgtga aagccactcc cagccagaac gaaacaattt
     7321 tctggcacgt cgtgagcggt gcttacggaa aagcagcaag cgcgctgtat cgggtgatga
     7381 gtggtatctg tcagggaatt acgtgggggc ttaatgagta ataaatattg ccaggagctg
     7441 gtggagctgc ggaacaaacc agcccatgaa ctgaaggaag tgggcgatca gtggcgcacg
     7501 ccggataaca ttttctgggg aattaacacc ctgtttggcc catttgtcct ggatctgttt
     7561 actgacggtg ataacgccaa atgtaccgcg tattacacgg cggaagacaa cgcgctggcg
     7621 catgactggt cagaacgtct tgcggagctt aaaggtgctg cctttggtaa tcccccatac
     7681 agccgcgcca gtcagcatga ggggcaatac atcaccggca tgcgttacat catgaaacat
     7741 gccagtgcca tgcgtgataa aggcgggcgc tatgttttcc tgatcaaagc tgccaccagc
     7801 gaagtgtggt ggccggaaga tgcagatcat attgctttta ttcgcgggcg tattggtttt
     7861 gaactgcctg cctggtttat cccgaaagat gagaagcagg tgccgacagg cgctttcttc
     7921 gctggtgcta ttgctgtttt cgacaagacc tggaagggac cggcaatcag ctacatcggg
     7981 cgcgatgaac ttgaggcatg tggtgaggcg tttctggcgc aggttcgcca gcaggcggaa
     8041 aaactggtca gggagatggc ggcatgacga cgttaactca atgccagcag caggtgctgg
     8101 atatgctgat ttcttatcag aaagaacgtg gcttcccgcc aaccaatcag gaggtggcaa
     8161 ccatgctggg ataccgttca gtgaatgcag cggtggagca tcttcgcgca ctggagaaaa
     8221 aaggcgtcat cacgataaag cgtggtgtgg cccgggggat cacgcttcat accgtggtga
     8281 aggacgacga cagcgaggcg gtcgggatta tccgctcact gcttgccggt gaggaaaacg
     8341 ccaggctgcg tgcagcccat tggttacatg agagaggcct gaaagtatga agctgatcct
     8401 gccttttccg cccagcgtga acacgtactg gcgacacccc aacaaagggg cgtttgctgg
     8461 taagagcctg ataagcgcgg cggggcgaaa attccagagc gcggcgtgtg cagcaatagt
     8521 tgagcagtta cgtcgtctgc cgaaaccaac gtcggcacct gcttcagtgg agatcgtgtt
     8581 gtttcctccg gataaccgga tccgcgatct ggacaactat aacaaggcgc tgtttgacgc
     8641 cctgacccac gcgggggtgt gggaagacga cagtcaggtg aaaagaatgc tggtggagtg
     8701 gggaccggtt atcccggagg ggaaggtcga gatcactatc agtaagtacg aaaaagcgag
     8761 ttgcaaatta gcaactcggt aacggaattg agcaacaccc taaatttggg tattacctcg
     8821 ttaaagatac tgtatttatg aacagtgtat ccttgataac tattaaaaat cgcagtaagt
     8881 tcatcctgca tcaacgaaaa gggagtgcag tcccgctcgt ggataaaaat ttgtggagaa
     8941 accaatgaat cagttgcttg taattgatgg cgtttctgtg cgccagtact tcgaatctaa
     9001 ctactgtctt aacgaccttc agaaagctgc tcttcttgcc gctggtgaga atcgctcctc
     9061 ccgttcgctg gaagttcacg agtttatgcg tcgtcctgaa acgaaggctc ttgtggaatt
     9121 attggaagaa gaaactacgg gagattcccg tagtattcct gtcatcacca ttcaggggcg
     9181 caatggtggg acgtatgtct gtaaagagct ggtctatgca tatgcaatgt ggatcagccc
     9241 ggcattcagc ttaaaagtga tacgtacttt tgatgcgctt cataattcat caccagaaga
     9301 aaccacatcc gacaaaatta aatccggggt cattctgctt gaatcagcag caaagactct
     9361 aaatctgtca aactcctcga aacttggtgc ataccagaaa ttatcaaagg tagctggtct
     9421 tcctgaactt atgccgatct atgccattga tgcacctgct gatgcgccag atggttcaag
     9481 ccgccctacg ctgtcgctga gtgcactgct gaagcagtat ggtatccgcc tgacggctaa
     9541 tcaggcatat caccagatgg cgaagctggg gatcgttgaa caacgcgaac gatacagccc
     9601 taccgcgatt aacaacatca aaaaattctg gtcgctgacc gcgaaaggct gcatgttcgg
     9661 caagaacatc accagtcctg caaatccgcg cgagacgcag ccgcatttct tcgaatcccg
     9721 attccctgag ctgttaaagc tgctcgatac cgttcattga ggtgaccgtg agagcactac
     9781 tgacccctga aatcgccccg cgtatgggga tcgtattgtt caggccaggt tcagagctga
     9841 tgcccctgtt tatgcagggg cgtgtcctgc tggagcctga gccggagcgt tattcatctt
     9901 tcgccagtgg tgccgttccg gcggcatcac aaccgctggc ggatgatcct gccgttcggg
     9961 ccgtgttccg caatgaggca gtgatccgtc gtgctggtgg cgtggaatgt cttgaaagct
    10021 ggttacttcg tgaaaaaggc tgccagtggc ctcattccga ctggcacagc gagaacatga
    10081 ccacaatgcg acacgctccg ggcgcaatcc gtctgtgctg gcactgcgat aaccagctgc
    10141 gcgatcagtt cacggaacgg ctggaatcaa tggcgacgga taactgtgcc cgctgggtgt
    10201 tgtctgttgt gcgtcgggat ctcggttttg atgatagtca cgttgtgaca atgccggaac
    10261 tgtgctggtg gctgattcgt aatgacttgg cggatgcctt accggaaagt gcagcccgta
    10321 aggcactgag attaccaaat cttgttgtgc cgtctgtcac ccgggaaagt gaccttgtgc
    10381 cttcggttcc tgccaccagc atcatccagg ataaggcaaa aaaggtgctg gcgctgaaag
    10441 tggatccgga gtcgccggag tcttttatgt tacgcccaaa acgtcgccgc tgggttaatg
    10501 aaaagtacac gcgctgggtt aagacacagc cgtgtgcatg ttgtggaaag cccgctgatg
    10561 atccccacca cctgataggt cacggtcagg gtggaatggg aacaaaagcg catgacctct
    10621 ttgtgttgcc tttgtgtaga aagcatcacg acgagctgca tgcggatacc gtggcattcg
    10681 aagagaagta tggctcccag ctggagctga tatttcgttt tatcgatcgt gcgctggcaa
    10741 taggcgtact ggcgtaagtg gagaacgagc atgaaccttg aagccttacc aaaatattac
    10801 tccccaaaat ctccaaaatt gagcgatgac gcaccggcga caggctcggg tggtttaacg
    10861 attacagatg tgatggctgc gcaggggatg gtgcagtcga aagcaccgct tgggtttgcc
    10921 ttattcctgg caaaagttgg tgttcaggat cctcagtttg cgattgaagg tctgctcaat
    10981 tacgcgatgg cactggataa cccgacattg aataaattga gtgaagaaac ccggttacag
    11041 atcatccctt accttgtgaa ttttgccttt gctgattatt cccggtctgc ggaaagtaag
    11101 gctcgctgtg agcgttgtgc tggtactgga tttcataatg tattgcgcga agtggtgaaa
    11161 cactccagaa gcggggaatc tgttatcaag gaagagtggg tgaaggaact atgtcagcat
    11221 tgccatggta agggagaagt cagcacagcg tgcagagggt gtaagggtaa aggtattgtc
    11281 ctggatgaaa aaagaacccg gcttcatggc gcgcctgttt ataagatttg tgggcgttgc
    11341 aatggaaacc ggtttagccg tttaccaacc acactggcga ggcatcgtgt ccagaagctg
    11401 ataccagacc tgacggatta tcagtggtac aaaggatatg cagatgtcat tgataaactg
    11461 gttacaaagt gctggcagga agaagcgtat gctgaagagc aattgagaaa agtgacaaga
    11521 tagataattt cgccgaagat gtcgacatga tgcttgcatt tttcaaaaaa tatggataaa
    11581 gttttcccaa cgatgggctt tgtatgtcta ccgttgataa gatttaagag cccgccgctg
    11641 agcgggtttt tgttgcatct cctgttaaat accgccatta gctcatcagg atagagcgcc
    11701 agccttcgaa gctggttgcg cggggttcga gtcctcgatg gcggtccatt atcggtacac
    11761 tgcgttgtta gctcagccgg acagagcaat tgccttctaa gcaatcggtc actggttcga
    11821 atccagtaca acgcgccata attatttacc aggctcgctt ttgcgggcct tttttatatc
    11881 tgcgccgggt ctggtatcgt attacttcag ccaaaaggaa tacctgtata tgaagtgtat
    11941 attgttaaaa tgggtactgt gcctgttact gggcttttct tcggtatcct attcccggga
    12001 atttacgata gacttttcga ctcaacaaag ttatgtctct tcgttaaata gtatacggac
    12061 agaaatatcg accccacttg aacatatatc tcaggggacc acatcggtat ctgttattaa
    12121 ccacacccca ccgggcagtt attttgctgt ggatatacga gggcttgatg tctatcaggc
    12181 gcgttttgac catcttcgtc tgattattga gcaaaataat ttatatgtgg ccggattcgt
    12241 taatacggca acaaatactt tctacagatt ttcagatttt gcacatatat cagtgcccgg
    12301 tgtgacaact gtttccatga caacggacag cagttatacc actctgcaac gtgtcgcagc
    12361 gctggaacgt tccggaatgc aaatcagtcg tcactcactg gtttcatcat atctggcgtt
    12421 aatggagttt agtggaaatg ccatgaccag agatgcatcc agagcagttc tgcgttttgt
    12481 cactgtcaca gcagaagcct tacggttcag gcaaatacag agagaatttc gtctggcact
    12541 gtctgaaact gctcctgttt atacgatgac accggaagaa gtggacctca cactgaactg
    12601 ggggagaatc agcaatgtgc ttccggagtt tcggggagag ggtggtgtca gagtggggcg
    12661 aatatccttt aataatatat cagcgatact gggcacagtg gcggttatac tgaattgcca
    12721 tcatcagggg gcacgttccg ttcgctccgt gaatgaagag atacaaccag aatgtcagat
    12781 aactggcgac aggccagtta taaggctaaa caatacttta tgggaaagta ataccgcagc
    12841 tgcttttctg aatcgcaggg ctcactcttt aaatacatcc ggagaataac gggagttaaa
    12901 tatgaagaag atatttgtag cggctttatt tgcttttgtt tctgttaatg caatggcagc
    12961 tgattgtgca aaaggtaaaa ttgagttctc taagtataat gagaatgata cattcacagt
    13021 aaaagtggcc gggaaagagt actggactaa ccgctggaat ctgcaaccgc tactgcaaag
    13081 cgcacagtta acaggaatga cggtaacaat caaatcaaat acctgtgcgt caggttcagg
    13141 atttgctgaa gtgcagttta attaatatca gaagcattgc tggtttcgta gtgtgcagca
    13201 atgtagttac agtgtaatca atgtcacaat tcagtcagtt gacggttgcc tgtctggctg
    13261 agcatttgtt aaaaaaatcc tgcatgatga atccccctgt gcggaggggc ataatgacag
    13321 atgtttggtt gcgtattgta taggcaagtt gcggattctg tctggtcatt gcagaattca
    13381 ccgggaggca cccggcatca tgctgtatac agagattagg catatatcca ggctcctcat
    13441 cgcaggagcc tttttacatg caaaaaaagc ccgcgccggg agacgcgggc ggcaaggaat
    13501 aaacaacaaa acgtgaagta atatttcagc tggcgaataa tacccgacag taatcactct
    13561 gcgcaactgc gcggcctttt tcgtattgcg ggctgtagtc ttccttctgt cattgtcctt
    13621 taacttccgg acctcagccc gctctttatc tgattcagta cactatcccg gccgggagga
    13681 ttcatgacat ttaaacacta tgacgtggtc agggcggcgt cgccgtcaga ccttgcggat
    13741 gcgcttgctc aaaaaattcg tgaaggatgg cagccatacg gcgggccgtt ttcttcgtat
    13801 acggatgatg gcgcagcact tattcaggcg attgtcgcag aaggtgatgt gagcacacct
    13861 gtgttggtga agccgtcgga tggagaaggc acagttatca gcaccaccag cgaaccggag
    13921 tattactttg ttgtggttct ggcggggcag tcaaacagca tggcatatgg tgaaggcctt
    13981 ccgctgccgg agacatatga ccgtccggac
//
LOCUS       DAZGXZ010000126        13774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000126 DAZGXZ010000000
VERSION     DAZGXZ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.126"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..552)
                     /locus_tag="ACHKJF_004064"
     CDS             complement(<1..552)
                     /locus_tag="ACHKJF_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951329.1"
                     /translation="MCDFIKSCYAAPRINKMADLFSSTASGVKHKPLKHVKIDNRLFP
                     SPEYIIFSDYLTKNSGPFVNHYFCSIPYSKEEECRLGVTIMKYASHRNKPLSLWCLGM
                     AEGAMARTISQLSKGQVLSLTTSPTKENEPVFFSHGSHIHSYFICSPFFLVHEEIKNN
                     NPELFPHGFDIIIEDTTFQMYSPD"
     gene            1259..1651
                     /locus_tag="ACHKJF_004065"
     CDS             1259..1651
                     /locus_tag="ACHKJF_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951330.1"
                     /translation="MSTFILYPEPGISSFFRRMTAQLRVFFSRNNLSYYSRWYTNPAG
                     NLSVRVCMFRRGGVWTPCLYYRQGMYRNEYTGWYQPLPREVIYLNRFTGRISRRRTWH
                     AACRMAEQLTTLRYRFGLQGDGIVAQPC"
     gene            1734..2213
                     /locus_tag="ACHKJF_004066"
     CDS             1734..2213
                     /locus_tag="ACHKJF_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951331.1"
                     /translation="MHSLLILLYVVVWRACTILRVIAPVSAVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYTESIARHLGYKSGGDQLKSGLFRPFLWHIRVKGHRLGAVM
                     EMEAWKTWKNGCNDVCNPWEDVCNPWERGWNDGWTECLNQRIAGLLKQACIEFSDAD"
     gene            2409..2786
                     /locus_tag="ACHKJF_004067"
     CDS             2409..2786
                     /locus_tag="ACHKJF_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000287229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951332.1"
                     /translation="MYSPLIAHYSALQTQSALLAHISQMEGELMRLRAWQRLGITPEP
                     DDETEPSLVYVYLWDTGEALGWLLYDLEQEEISAPGIQARQALDSLMALGREINHEIW
                     ADSISTGKMVAGADEYFARHDMM"
     gene            2868..3497
                     /locus_tag="ACHKJF_004068"
     CDS             2868..3497
                     /locus_tag="ACHKJF_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951333.1"
                     /translation="MSELSSRPAREPVVYTLEQVSTIPEKQWHAFVLAVTETFWQLPE
                     ALRPQNAYFGSLTRASELFPVTDTLAFYCRSADGLWSVNVTIEREHSRNILALNELNF
                     GRQPGDFFARTVFVLLHNLCPDCFRIHSTVGGASWSLPLKWIKRYLGHENFSAPESVL
                     TTPVRGDAFDSLLLQFLSGQGRQLSPDDWAALEEAEYQLYWLRALAGGR"
     gene            3613..3996
                     /locus_tag="ACHKJF_004069"
     CDS             3613..3996
                     /locus_tag="ACHKJF_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951334.1"
                     /translation="MGLDIYFLARDNHHSGAVVGTQEHQEVGAFRKVKPLLTWFREHT
                     GASDEQHAFPVTRSSLAALLRDLSMLTPGNCHRLFPYVTGSCLRGEDYAGDYWYDVDD
                     VKAWVSNTLKTFDFERRSLVMLALW"
     gene            4089..5066
                     /locus_tag="ACHKJF_004070"
     CDS             4089..5066
                     /locus_tag="ACHKJF_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFP0951335.1"
                     /translation="MKTKKHTSRTATKTPERDLYQLVTDRVVTAIENGVPPWKRPWRS
                     ARNGVGLPEMMPANALTGKLYTGVNVLLLWLAADEAGYVSNDWLTYRQAQQLGGHVRK
                     GEVATLAVIYKDWKKQAVDEHGNRLTDSEGEPLMEIVPVLKSNPLFNVGQCDGLPEHL
                     MVKAEGSADALPADTFSERVLQVLNGSGVSVSSLPQDRAFYRPDLDRIVMPLSSQFDQ
                     VADYWTTLLHELVHSTGHEKRLRRVGISTFTGRNSPEYAFEELIAEMGSAFLCAHLGI
                     AGDVQHESYIDGWLKVLKSDKKALFRACRQAREASEYLLTRRTSQECAA"
     gene            5180..6127
                     /locus_tag="ACHKJF_004071"
     CDS             5180..6127
                     /locus_tag="ACHKJF_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0951336.1"
                     /translation="MSEWCKNRFEITGKSVCLDVLTQWIEGRETPRYRHAIQQSIQLF
                     LAGCAGLVKPTRTTQYPPYPALVRHGTGLSSPANQAFEQWLGLLVKNAVLDGETIKTI
                     ERLYHQSCIGAVRWGNLPDNAREIITTLMSRQYSDWFGLAGLSEHIDAEACWSWLSDY
                     PEQAQPCDMLMVIPSRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGNNVRHARDE
                     MSSLVLLIDTPWYPPSGELVGEISKVFDCEVRHWYSEPVHGIQGYNCYDGGEHTDSDL
                     QAEWPGRETLPQPRLYLVEECAEEQTKAAPLPVPLVSAQ"
     gene            6239..6925
                     /locus_tag="ACHKJF_004072"
     CDS             6239..6925
                     /locus_tag="ACHKJF_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0951337.1"
                     /translation="MATMTDHQKEFISLFNRTACYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVIHIAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWSIASMMAQMQMGDVKALFENKPFITLSEPACGAGSMILAMADILNRSGYPAY
                     HRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRVLLTPGHYLGNWDHR
                     LRHLQEQNVA"
     gene            complement(7159..7711)
                     /locus_tag="ACHKJF_004073"
                     /pseudo
     CDS             complement(7159..7711)
                     /locus_tag="ACHKJF_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154742.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            7829..8746
                     /locus_tag="ACHKJF_004074"
     CDS             7829..8746
                     /locus_tag="ACHKJF_004074"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0951338.1"
                     /translation="MQCEFKGVISALPTPYDQSQQIDMESLRKLIRFNIEQNIKGLYV
                     GGSTGEAFLQNVAEREKILETVADESDGRLTLIAHVGGISTAESEVLAKAAKKYGYHA
                     ISAVTPFYYPFSFEEHCIHYRKIIDSADGLPMVVYNIPALSGVRFSLDQINELVTIPG
                     VCALKQTSGDLFQMEQIKRNHPELVLYNGYDEIFASGLIAGADGGIGSTYNIMGWRYL
                     EIFEAVKNNDVIKAKEMQVACNQVIDTLIQSGVLAGIKTLLYYMGIINTPVCRSPFSP
                     VKEKNLDVLSKLAERLLEEHDRNKKMKII"
     gene            8779..9654
                     /locus_tag="ACHKJF_004075"
     CDS             8779..9654
                     /locus_tag="ACHKJF_004075"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000629094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009384 - N-acylmannosamine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0951339.1"
                     /translation="MITLAVDIGGTKISAALISDDGSFLLKKQISTPHERCPDEMTGA
                     LRLLVSEMKGTAERFAVASTGIINNGVLTALNPDNLGGLKEYPLKNIMEDITGLNGSV
                     INDAQAAAWAEYTVLPKEICDMVFITVSTGVGGGIVVNRKLLTGVSGLAGHVGHILSG
                     VTDTECGCGRRGCVEAVSSGRAIMGAAKNELAGYSTKYIFELARQGYKEAEFLTERSA
                     STIAELIVSLKLLLDCQVVVVGGSVGLADGYIQKVSKHLSIYSEICNVMLFPAYFRSD
                     SGLIGATLWDRDCIT"
     gene            9703..11175
                     /locus_tag="ACHKJF_004076"
     CDS             9703..11175
                     /locus_tag="ACHKJF_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951340.1"
                     /translation="MDSCIQKKQVWYKHLSPRQWKIFGAAWTGYLLDGFDFVLISLVL
                     TEVQHEFGLTTIEAASLISAAFISRWFGGLAIGALSDKMGRRMAMVLSIVLFSLGTLA
                     CGLAPGYAVMFIARIVIGLGMAGEYGSSVTYVIESWPVHLRNKASGFLISGFSIGGGL
                     AAQVYSIVVPLWGWRSLFFVGMLPILFAFYLRKNLPESDDWQKRQQENKPVRTMVDIL
                     YREKNKYINILLSCIAFACLYVCFSGVTANAALITVMALCCAAVFISFIYQGMGKRWP
                     TGIMLMLVVMFCFLYGWPLQAFLPTWLKVDMQYSPETVALIFMLAGFGSAAGSCIGGF
                     MGDWLGTRKAYVISLLIGQLVIIPVFLVDRDYVWLLGLLIFTQQVFGQGIGALVPKII
                     SGYFNVEQRAAGLGFIYNVGSLGGACAPILGAVVASHTSLGTAMCSLAFILTFVVLVL
                     IGFDMPSRVQRWIHPEAALEYDTVDGKPFYGARKKNVAGE"
     gene            11179..12009
                     /locus_tag="ACHKJF_004077"
     CDS             11179..12009
                     /locus_tag="ACHKJF_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000948499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0951341.1"
                     /translation="MLSGNKKMIHYRENIYSLDNYERYITDNSLFENTDDGIIYTGIV
                     PLITVVEPVVSNQHSVLIIPGGGYRRIVYEREGLDVGRWIAGQGYTAFILSYRMPSVN
                     GYYSFADAKNALNFISYWAGMSERTFKSVGCMGFSAGGHLAGYITHLHYEEETSVRPD
                     WMALIYPVISLDRSIAHQGCRKRLLDAGESEYSASLENSVTEYMPPVFIVHATDDKDV
                     PVIHSLRYFNSIRDKGHHAEAHFFDKGGHGFGVSGKNGYPIEKWQSLLLEWLAEHKFH
                     "
     gene            12055..12765
                     /locus_tag="ACHKJF_004078"
     CDS             12055..12765
                     /locus_tag="ACHKJF_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligogalacturonate-specific porin KdgM family
                     protein"
                     /protein_id="HFP0951342.1"
                     /translation="MKKYYPLLTVILLGLPLCSQALTLNLRGGYRSASHAYETLIQVS
                     QGWNNGWWASFESNTWNTIHNNENELFALNYNKIDINKTYKIDDKWALIPGIQTQWMS
                     AGSQYNPYLKISYQATPDLNLALRYRYDWKAFRQTDLDGNRARNDQHQFDGYVTYKVN
                     DSWLFAWQTTVYTKVNNFKYGNHKKTATENAFVLQYKMSPVFTPYIEYDYLDKQGVYK
                     GKDNKHENSYRVGVLINF"
     gene            12778..>13774
                     /locus_tag="ACHKJF_004079"
     CDS             12778..>13774
                     /locus_tag="ACHKJF_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0951343.1"
                     /translation="MKVKNFIYLPFCLFIGTSVAGALPEIPEPFKYGVGGIDNGKIYI
                     GLGSLGNNWYMIDTNQSEKKWTKIAQWPTVPREQATATIIDGKIYVFGGIGKDKSGVI
                     TLQKDVYSYDIAKDKWEKLMTRPPVSLAGHVSFIHNGHAVSTGGVNENIFNGYFSDVE
                     LSKGNSALTEKVNRDYFSKPVDDYFLNNHMISYDPSKNQWKNLGTTPFPGTAGSSVIF
                     AEQQIYILGGERKPGLRSVRSWTGELSHDRIKWSELPPVASPEGVSGAYASVIDGNIF
                     LAGGAYFPGAAEKYSNGEYWSHKGLDKAYSKEIYQLIKNDWKKVGSLPEGLAYGVS"
BASE COUNT         3527 a         2682 c         3586 g         3979 t
ORIGIN      
        1 gtcgggagaa tacatctgaa acgttgtatc ttctattata atatcaaaac catgaggaaa
       61 tagttcaggg ttgttatttt ttatttcttc atgaacaaga aagaatgggg agcaaataaa
      121 gtaagagtga atatgagaac catgactaaa gaatacaggt tcattttctt ttgttgggct
      181 ggttgttaat gataaaacct gtccttttga tagttgagaa attgttctgg ccatggctcc
      241 ttctgccatt ccaagacacc ataagctcaa tggcttattt cgatggcttg catatttcat
      301 aatagtcaca cccagcctgc attcttcttc tttgctgtat ggaatactgc aaaagtagtg
      361 gttaacaaat ggaccactgt ttttcgtaag atagtcagaa aaaattatat actcaggaga
      421 tgggaaaagc ctgttatcaa tttttacatg cttcaaaggt ttatgtttta ctccagatgc
      481 tgttgatgag aaaagatcag ccattttgtt aattcttggt gctgcataac atgattttat
      541 aaaatcacac atttaactat cctgtataac ttataacatc agtaaatcag gagcatatac
      601 tatttttctg aagtatcaaa cagaatctgc ttatttgaaa taagctgtta tctctgtgga
      661 tgtttacttg atatatgata caaggatgtg tggattgtat agaatatttt tttacattca
      721 cccatttaag acaaaacaaa tcactttaca tactgttgat gttaattttc acaatgtcct
      781 ttttagttgt ttattcagtg agttccgata gcaggtgtta agagttcagc ttctgtttcg
      841 tgagggagca tggataattg acgcctttta tgcgctctga tatgctcaga atgcattatc
      901 tgtcgcttcg gcgaccacca atgctccata agtggtctga gaggagcgcc ttcgggtgac
      961 cacatcccgc ttcggtgggc accaatgttc cgttgatctg agaggaacgc cttcgggtga
     1021 tcaacacccc cgtaacctga aaggggatgt gcagccagga tgtttctggt ggtggtaaat
     1081 ctgcccacct ttgagagcag atatcgtcag tcatgacgat gaaggtttct ttataactgc
     1141 gccctttatt aacgggcttt ttctgacagc aacaccatgc tgtactggtg gtgttttaac
     1201 cctgacggga gtttttgtcc gtcggggcga aggctcccgt ttttttacag gagtctttat
     1261 gtcaacgttt attttgtacc ctgaaccggg catctcttct tttttccggc gaatgacggc
     1321 tcagttgcgg gtgttttttt cccgcaataa cctgtcatat tacagtcgct ggtacactaa
     1381 tccggccggc aatctgagcg tgcgggtctg tatgttccgt cgtggcggtg tctggacacc
     1441 ctgcctgtat taccggcagg gaatgtatcg caacgagtac acaggatggt atcagccgtt
     1501 accgcgtgag gtcatttatc tgaaccggtt taccggacgg atatcccgtc gcaggacatg
     1561 gcatgctgcc tgccgtatgg cggagcaact gaccaccctg cgttaccggt ttggtctgca
     1621 gggtgatgga atcgtcgcac agccctgctg atttatcatt tattaccctg tcgggaacac
     1681 gcttcccgca gggcaggtgt gttcccgtta ttttttgtat ctgaggtgaa cacatgcata
     1741 gtctgttaat actcctttac gttgttgtat ggcgtgcctg caccattctt cgggtaatcg
     1801 caccagtgtc ggctgtccgt gcgtggtgtg agtatcaact ggaaggtatg acgatacttc
     1861 gtgatgcgta cagctcgctg gctaaggcgg ctggaatgca gaatgatgca tttgaatata
     1921 ctgaaagtat tgcacgtcat ctgggctaca aaagcggtgg tgaccaactg aaatcaggac
     1981 ttttccggcc gtttctgtgg catatacgtg tgaaaggcca caggctgggg gctgtcatgg
     2041 agatggaggc atggaagaca tggaagaatg gctgtaacga tgtctgcaat ccatgggagg
     2101 atgtctgcaa tccatgggag cgtggatgga acgatggctg gacggaatgt ctgaatcaac
     2161 gtatagccgg gctcctgaag caggcctgta ttgaattttc cgacgcagac tgacgggcag
     2221 gatgccagtc agtcacagga aggatgtcaa atcgtgagcg atgcaactgc cattttgcaa
     2281 tgatggctgt tttgctcccg taacggtttt tcttttactt actgcaccgt gtttttgctc
     2341 cggtgttttc cctgacggga gttttgttcc gtcagggaca ggacttcctt taagagacgg
     2401 aggtcatcat gtattcacct ttaattgctc attattcagc gcttcagacg cagtctgctc
     2461 tgctggctca tatcagtcag atggaaggcg agctgatgcg gttacgtgcc tggcaacggc
     2521 tgggtattac cccggagccg gatgatgaga cggagccttc actggtatac gtgtatctgt
     2581 gggataccgg cgaggcgctg ggctggctgt tgtatgacct ggagcaggag gagatttccg
     2641 caccgggaat tcaggcccgt caggcactcg acagtctgat ggctctgggg cgtgaaatta
     2701 atcatgaaat ctgggctgac agtatcagta ccggaaagat ggtggccgga gcggatgaat
     2761 attttgcccg gcacgacatg atgtaattca tgacctgacc ctgacggggg agttctccgt
     2821 ccgggagaca ttccccgttg tttcgtttta accatgagga atgttccatg tctgagttat
     2881 cttcacgtcc ggcccgtgag ccggtggtat atacccttga gcaggtttcc acaataccgg
     2941 aaaagcagtg gcatgctttt gttctggctg tcactgaaac cttctggcag ttgccggagg
     3001 cgttacggcc gcagaatgcg tatttcggct ccctgacccg ggcgtcagag ttgtttccgg
     3061 taacggacac gctggcgttt tactgccggt ctgccgacgg gctgtggtcg gtgaatgtca
     3121 ctattgagcg ggagcacagc cggaatattc tggctcttaa tgagctgaat ttcggacgtc
     3181 agccgggaga tttctttgcc cgtacagtct ttgtgctgtt gcacaatctg tgtccggact
     3241 gttttcgtat tcattccact gtcggtggtg ccagctggtc gctgccgctg aaatggataa
     3301 aacggtacct ggggcatgag aatttttctg ctccggagtc tgtattaacc accccggtaa
     3361 ggggagatgc gtttgacagc ttactgctgc agtttttgtc cggtcagggg cgacagttat
     3421 ccccggatga ctgggcagca ctggaagagg ccgaatatca gctttactgg ctgcgggctc
     3481 ttgccggtgg gcggtaacag aacgccgtag tttctttatc cggaataacg tcatcacgta
     3541 ttccgtcatt atatcacccg gcagggacat atccctgtgg ggagagtccc ggctttattc
     3601 aggagaattg ttatgggact cgatatttac tttctggctc gtgataacca tcacagtggt
     3661 gccgttgtgg gaacacagga acatcaggag gtgggggctt tccgtaaggt gaaaccgctt
     3721 ctgacatggt ttcgtgaaca taccggtgct tcagatgagc agcatgcttt ccctgtaacc
     3781 cgcagttcac tggctgcatt attgcgcgac ctcagtatgc tgacaccggg gaactgccac
     3841 aggctgtttc cttatgtcac aggctcgtgt ttacgggggg aagattatgc cggtgattac
     3901 tggtatgacg tggatgacgt aaaagcgtgg gtcagcaata cgctgaagac gtttgatttt
     3961 gagcgtcgtt cactggtgat gctggcgttg tggtaattct ggccagtctg attttcgtta
     4021 ttaatttaac tgtacggtgc actctcaccg tacagggagc gtgcatcttt ttcagggggc
     4081 atgctcttat gaaaacgaaa aaacatactt cacgtaccgc gactaaaact ccggaacgtg
     4141 acctgtatca gcttgtgacc gacagggttg ttacggccat tgaaaatggt gtgccaccct
     4201 ggaaacgtcc ctggcgctct gcgcgtaatg gcgtggggct tccggagatg atgccggcaa
     4261 atgccctgac cgggaagctt tatacagggg tgaacgtcct gttgctgtgg ctggccgcgg
     4321 atgaagccgg gtatgtcagc aatgactggc tgacgtaccg tcaggcgcaa caactgggtg
     4381 gccacgtccg taaaggggag gtggccacgc tggcggtgat ttacaaagac tggaaaaaac
     4441 aggctgtgga tgaacacggt aaccggctga cggacagtga gggggagcct ctcatggaaa
     4501 tcgtaccggt actgaaatcg aatcctctgt ttaatgtggg gcagtgtgac ggtttacctg
     4561 aacatctgat ggtgaaggcg gaaggcagcg cggatgcact gccggcagat accttcagtg
     4621 agcgggtgct gcaggtactg aatggcagcg gcgtcagcgt ctcgtcactt ccacaggaca
     4681 gggcattcta ccggcctgat cttgaccgta tcgtgatgcc actgtcgtcg cagtttgacc
     4741 aggtggctga ttactggacc accctgctgc atgagctggt acacagtacc ggtcatgaaa
     4801 agcgcctgcg gcgggtgggg ataagtacct ttaccggtcg caacagcccg gaatatgcgt
     4861 ttgaagagct gattgccgag atgggcagtg cttttctgtg tgcacatctc gggattgccg
     4921 gtgacgtaca gcacgaaagt tatattgacg gatggcttaa ggttctgaag tctgacaaaa
     4981 aagcgctgtt tcgcgcctgc cggcaggcac gtgaagcatc agagtattta ctgacgcggc
     5041 gaacttcgca ggagtgcgca gcataatttt tactaccggt ggcggttata acctgaccgc
     5101 catttatccc gtgagggcat tgtacctcac gggacaatgc cctcttttta catctgtata
     5161 aaaaagagga gcgtaaacaa tgtctgaatg gtgcaaaaac cgttttgaaa ttaccggtaa
     5221 atcagtctgt cttgatgtac ttacacagtg gattgaaggg cgtgagaccc cgcgctaccg
     5281 tcatgccata cagcagagta tacagctgtt tctggccggt tgtgcagggc tggtaaaacc
     5341 cacacggacg acgcagtatc cgccatatcc ggcgctggtt cgtcacggaa cggggttgtc
     5401 atctccggct aaccaggcgt ttgagcagtg gctggggttg ctggtaaaaa atgccgttct
     5461 ggatggtgaa acgataaaga ccattgagcg tctttatcac cagtcatgta tcggcgctgt
     5521 tcgctggggt aatctgccgg ataatgccag ggagatcatc accacgctga tgagccgtca
     5581 gtattctgac tggtttggtc tggccgggct gagtgaacac attgatgcag aagcctgctg
     5641 gagctggttg tcggattacc cggaacaggc gcagccctgt gacatgctga tggtcatacc
     5701 ctcccgtctg gctacagagc tgaatggttc aggtggactg ctgcaaggta tttccacaac
     5761 gaccagcctt tacagccgga tatacggtgt ggagtggccg tcaggaaata atgttcgcca
     5821 tgcgcgtgat gagatgagca gtctggtgct gctgatcgac acgccctggt atccgccttc
     5881 aggcgaactg gttggtgaga tctcgaaggt gtttgactgc gaggtccgac actggtacag
     5941 cgagccggta cacggtatac agggttacaa ctgttacgat ggcggagagc atacggacag
     6001 tgatctgcag gccgaatggc cgggcaggga aacacttcca cagccccgac tgtatctggt
     6061 ggaggagtgc gctgaagaac agacgaaagc cgcaccttta ccggttcctc tggtctcagc
     6121 ccagtaatta acctccgggt atcatgtccg tgatgcccgt tttatcaaca tttcaacccg
     6181 aaggggaaca cctgttcccc tgtccgggcc atgtgttccc tgtttttacg gagaaaacat
     6241 ggccaccatg actgaccatc agaaggagtt catttcgttg tttaaccgga cagcctgtta
     6301 tcacaaacgt cataaggtgt ttgaagactt tgtcagctgt agtgtgattg ccctggaaaa
     6361 ccggcttcag ttcagtgagg cccgggaaca gaagtatctg cgaatcgtca gtggttatga
     6421 aaagcaggat gttatccaca ttgcgcattt gcttgctcat gtcattgagg gactggagca
     6481 gggattctgt gattttctgg ggagtgtgtt catgcaactg gaactggggg ataaataccg
     6541 gagtcagttt ttcacgccct ggagtattgc cagcatgatg gcacagatgc agatgggaga
     6601 tgtgaaggcg ttgtttgaga acaaaccctt tatcacgctc agtgaacctg cctgtggtgc
     6661 cggcagtatg attctggcaa tggctgacat cctgaaccgt tcggggtatc cggcatacca
     6721 caggatgtgg gtgtcggcaa cggatattga cccgctggca gccggcatgg cgtatataca
     6781 actgtcgttg tgtggtgtgg ccggagaagt ggtgattggt aactcgctct gtaatgagcg
     6841 acgccgcgta ctgctgactc ccgggcatta cctgggaaac tgggaccatc gtctgcgtca
     6901 tcttcaggag cagaatgtgg catgatgcag aatgttttat cactgcgcca gtcttcggac
     6961 tggcgttttg ttcaactgcc agttccctga atgtttgatt tgttcaataa agcctgttta
     7021 tcaataaatt ctctgacgac agggaatggt atgtttttat tagtttttat gtgttggaca
     7081 acaagacaga ataatgatgt ctgttttatg tatacgatat agccactgat tcaacgcatt
     7141 atcggctata ttgccagtct tatttagtgc cagattcatt gtatcgaagg ccgctgccgt
     7201 cagtagatgg ctggtcagtt ctatcagaca gaccattttt acctgaggat aaagtgtctg
     7261 gtttccacta tgtatttggc gagtgaagct ttatcgtgtt cttgcgtgtc ctgtgttcgt
     7321 cagaacacgc catcaatggt cagcagattc agatcgcacc agagcggata tggcgtggtg
     7381 ttatgctaga gctgcgctgt ttgagtgaac acgcagcgta tggctccgct tcctggccgc
     7441 tggcgggcat ggagcacggc gctactgcct ggcagcatga tgtccagccg gttcacaatc
     7501 tgatgaaggt tccttacgct catgggccat actgataatt caccagacca tcatttccag
     7561 aggaagacgc cgtttgtgta gcgtgacagt gtcggactct tcagggctgt gggtaatcag
     7621 ttccagggca agatagtcgc agagggttac gcagagaata gtaactggac actggatcga
     7681 tcaaccgatc cttaactgag cggcatgaca gctttctgat ggcttttttt tgataatctt
     7741 cacattgtgc ttcttttctg ttggataatc tttgtacttg atggtgtact aggtatccca
     7801 ttatgcagtt aagtgaaggt gattaaaaat gcaatgtgaa tttaaagggg tcatatcagc
     7861 attacccact ccttatgacc aatcccagca aatcgatatg gagagtcttc gaaaactgat
     7921 ccgttttaat atcgaacaaa atattaaggg attgtatgtt ggaggctcta ctggagaagc
     7981 atttcttcag aatgttgcag agcgagaaaa aattcttgaa acagtagccg atgaatcaga
     8041 tggaagacta acacttatag cgcatgtggg cggaatcagt acggcagaaa gcgaggtact
     8101 tgcaaaagct gcaaaaaaat acggatatca tgctatatct gctgttactc cattttatta
     8161 tcctttttca tttgaagagc attgtatcca ttacaggaag attattgatt ctgcagatgg
     8221 acttccgatg gtagtttata atataccggc tcttagcgga gtgcgttttt ctttagatca
     8281 gataaatgag cttgtcacaa tacccggggt ttgtgcatta aaacagacat ccggtgattt
     8341 attccagatg gaacaaatca aaaggaatca tccagagctt gtgttatata atggttacga
     8401 tgaaatattt gcatcagggt taattgcagg tgcagatggg ggaatcggta gtacatataa
     8461 tataatgggg tggcgttatc ttgaaatatt tgaagcagtc aaaaataatg atgtgattaa
     8521 ggcgaaggaa atgcaggtgg cctgtaacca ggttattgat actcttattc agagtggtgt
     8581 cctggcaggt ataaaaactc ttctttacta tatggggatt ataaatactc ctgtatgcag
     8641 aagtcctttt tctccggtaa aggaaaagaa tctggatgta ttatcaaaac ttgcagagcg
     8701 actgctcgaa gaacatgaca gaaataaaaa aatgaaaata atctgaataa ttgcaagatt
     8761 gttatatagg taaaatgaat gataacactg gctgttgata taggaggaac gaaaatttca
     8821 gcggcgttga tatctgatga cggttcattt ttgctgaaaa aacagatatc aacacctcat
     8881 gaaagatgtc ctgacgaaat gacaggggct ttacgtctac tggtttctga aatgaaggga
     8941 acagcggagc gatttgcagt agcatcaaca gggattatta ataatggagt attgacagct
     9001 ttgaatccag acaatcttgg agggctgaaa gagtatcctc tgaaaaatat aatggaagat
     9061 ataactggtt taaatggaag tgtgataaat gatgcccagg cagccgcatg ggcagaatat
     9121 acagttttgc caaaagaaat ttgtgatatg gtttttataa ccgtttctac gggggttgga
     9181 gggggaattg tagttaacag aaaattatta acaggtgttt ctggattagc cggacatgtt
     9241 gggcatatat tgtccggagt aacggataca gaatgtggat gtggtcgtcg gggatgcgtt
     9301 gaagcggttt catctggtcg ggccatcatg ggggcagcaa aaaatgaact tgcaggatat
     9361 agcaccaaat atatttttga attagcgaga cagggatata aggaagccga atttttgaca
     9421 gagcgttctg ccagtactat tgctgaatta atagtaagtt taaaattact gcttgattgt
     9481 caggttgttg ttgtcggagg aagtgttgga cttgctgatg ggtatattca gaaagtgtcg
     9541 aaacatcttt ccatatattc tgaaatttgt aatgtaatgc tgtttccggc atatttcaga
     9601 agtgactctg gtcttattgg tgcaactctt tgggaccggg attgtattac ttaaagtaat
     9661 actccagtgt attataaatc cgctattatt gtgaggttta atatggactc ctgtattcag
     9721 aaaaagcagg tttggtataa gcatctttca cccagacagt ggaagatatt cggtgcggca
     9781 tggacaggtt atttacttga tggttttgat ttcgttctga tatctctggt tcttacagag
     9841 gttcagcatg aattcggctt gacgacaata gaagcggcaa gccttatttc cgctgctttt
     9901 atttcccgat ggtttggggg gcttgctatt ggcgctttat cggacaagat ggggcgcagg
     9961 atggcaatgg ttttaagtat agtgctattc tctctcggaa cccttgcctg tggtctggct
    10021 cctggatatg cagtgatgtt tattgctcgc attgttattg ggcttggtat ggcaggtgaa
    10081 tatggctcaa gtgtaacata tgttatcgaa agctggcctg ttcatctcag aaacaaggca
    10141 agtggttttt taatttctgg attttctatt gggggaggtt tagctgcaca ggtttatagc
    10201 attgtagtac cactatgggg ctggcgttct cttttctttg tcggcatgct tccaatatta
    10261 tttgcttttt atcttcgcaa gaatcttccg gagtctgatg actggcaaaa aaggcaacaa
    10321 gagaataaac ccgtcagaac aatggtggat attctgtaca gagaaaagaa taaatacata
    10381 aatattcttc tgtcttgtat tgcatttgca tgtctatatg tttgtttttc aggcgtcact
    10441 gcaaacgctg ctttaatcac agttatggca ttgtgctgtg cggctgtttt tatcagtttt
    10501 atatatcagg gtatggggaa aagatggcca acaggcatta tgttgatgct tgtggttatg
    10561 ttctgtttcc tctacggatg gccactgcag gcttttcttc ctacatggct taaggttgat
    10621 atgcagtatt caccagaaac tgttgctctc atatttatgc tggcaggttt tggctctgca
    10681 gctggaagtt gtataggtgg ttttatgggg gactggcttg gaacccgaaa agcatatgtt
    10741 atatcacttc ttatcggtca gcttgttatt atccccgttt ttcttgtgga ccgggactat
    10801 gtatggttac ttggccttct gatctttact cagcaggttt ttggtcaggg gattggtgct
    10861 ctggtgccga aaataatcag tggttatttt aatgtggagc agcgtgcggc aggactgggg
    10921 tttatttata atgtaggttc acttggtgga gcatgtgcgc ctattcttgg tgcagttgtt
    10981 gcgtctcata caagtcttgg tacagcaatg tgttctcttg cttttatctt aacatttgtt
    11041 gttcttgtcc ttattggttt tgatatgcca tcacgtgtac agagatggat ccatccagaa
    11101 gctgcgcttg aatatgatac tgtcgatgga aaaccttttt atggagcaag aaaaaagaat
    11161 gttgcaggag aataaaggat gctatcaggg aataaaaaaa tgattcatta cagggagaat
    11221 atatattctc ttgataatta tgagcgttac ataactgaca attcgctatt tgagaatact
    11281 gacgatggca taatatatac aggtattgtg ccccttatta cagttgttga gcctgttgta
    11341 tctaatcaac atagtgtgct gattatacct ggaggtggat acaggaggat tgtttatgaa
    11401 agagagggac ttgatgtagg cagatggata gcaggtcagg gatatacggc atttattctt
    11461 tcttacagaa tgccctccgt taatggttat tattcctttg cggatgcgaa gaatgccctg
    11521 aactttattt cttactgggc cggaatgtcg gagagaacat ttaagagtgt tggatgtatg
    11581 gggttttcgg caggtggtca tcttgctgga tatattactc atctccatta tgaggaagag
    11641 acttctgtgc gtcctgactg gatggctctg atatatccag ttatatctct tgacaggagt
    11701 attgctcatc agggatgccg gaaacgtctt ctggatgcag gtgaaagtga atattcagcc
    11761 agtcttgaaa atagtgttac agagtatatg ccaccagtct ttattgtgca tgcaacagat
    11821 gataaggatg ttccagttat tcactctctg agatatttta actctatccg ggataaggga
    11881 catcatgcag aagctcattt ttttgataag ggagggcatg gatttggtgt atccggaaaa
    11941 aatggatatc cgatagaaaa atggcaaagc ctgcttctgg aatggcttgc ggaacataag
    12001 tttcattgag aaatcagatt acaagtgata aatatattta atgatgaggc atttatgaaa
    12061 aaatattatc cactattaac tgtgattctt cttggtttac ctttgtgctc tcaggcatta
    12121 actcttaatc tacgtggagg atacagaagc gccagtcacg cttatgaaac gttgattcag
    12181 gttagtcagg ggtggaataa tggttggtgg gcaagttttg agtcaaatac ctggaataca
    12241 attcacaaca atgaaaatga attgtttgca ctaaattata ataaaataga tataaataaa
    12301 acatataaaa tagatgacaa atgggctcta atcccaggga ttcagacaca atggatgtca
    12361 gcaggaagtc agtataaccc ttatctgaaa atttcgtatc aggcaacgcc agatcttaat
    12421 ctggccttac gctatcgcta tgactggaaa gctttccgac agacggattt agatggcaac
    12481 agggcgcgta atgatcagca tcagtttgat ggttatgtga catacaaagt taatgattca
    12541 tggttgtttg catggcagac cacggtatat acgaaggtta ataatttcaa atatgggaat
    12601 cataaaaaaa cagcaactga aaatgcattt gttctgcaat ataaaatgtc accggtgttt
    12661 accccatata tagaatatga ttacctggat aaacaagggg tgtataaagg gaaagataat
    12721 aaacatgaaa atagttaccg tgtcggtgtt ttgattaatt tttaaaaggt ggttacaatg
    12781 aaagtgaaaa actttatata tcttcccttc tgcttgttta ttggaacgtc ggtagccgga
    12841 gctttaccgg aaattcctga accatttaaa tatggtgtag gagggatcga taatggtaaa
    12901 atatatatag gactgggaag tctgggaaat aactggtata tgatagatac aaaccagagt
    12961 gaaaaaaaat ggactaagat agcacaatgg ccgacagttc ccagagagca ggcaacagct
    13021 actattattg atggtaagat ctatgtattt ggaggaattg gaaaagataa aagtggagtc
    13081 ataaccttac aaaaagatgt ctattcctat gatattgcta aagataaatg ggaaaaattg
    13141 atgacccgtc cccctgtatc tcttgccggt catgtttctt ttatccataa tggtcatgcg
    13201 gtatctactg gtggtgtaaa tgagaatatt tttaatggat acttttcaga tgtcgagtta
    13261 tcaaaaggga attccgcgct gacagagaaa gtcaacaggg attattttag taaacctgtt
    13321 gatgattatt ttttaaataa tcatatgatt tcttatgacc cgtcaaaaaa ccagtggaag
    13381 aacctgggaa cgactccatt tccgggaact gctggttcat cggtgatttt tgcagaacag
    13441 caaatttata tactgggagg ggagagaaaa ccgggccttc gttctgttag atcctggacg
    13501 ggggagcttt cgcatgatcg aattaaatgg tctgaacttc caccagtagc ttcacccgag
    13561 ggggtgtcag gagcatatgc ctctgttatt gatggaaata tctttctggc cggtggtgca
    13621 tatttcccag gggcggcaga aaagtatagc aatggtgaat actggtctca taaaggactg
    13681 gataaagcct attcaaaaga gatatatcag ctaataaaaa atgactggaa aaaagttggt
    13741 tctttgccgg aaggacttgc ttatggtgta agcc
//
LOCUS       DAZGXZ010000127        13753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000127 DAZGXZ010000000
VERSION     DAZGXZ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.127"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            71..981
                     /gene="hlyE"
                     /locus_tag="ACHKJF_004080"
                     /pseudo
     CDS             71..981
                     /gene="hlyE"
                     /locus_tag="ACHKJF_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1188..1634)
                     /gene="ycgN"
                     /locus_tag="ACHKJF_004081"
     CDS             complement(1188..1634)
                     /gene="ycgN"
                     /locus_tag="ACHKJF_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP0951344.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRDNLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1726..2385)
                     /gene="ycgM"
                     /locus_tag="ACHKJF_004082"
     CDS             complement(1726..2385)
                     /gene="ycgM"
                     /locus_tag="ACHKJF_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0951345.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIIPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2457..2750)
                     /locus_tag="ACHKJF_004083"
     CDS             complement(2457..2750)
                     /locus_tag="ACHKJF_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP0951346.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2992..3393
                     /gene="pliG"
                     /locus_tag="ACHKJF_004084"
     CDS             2992..3393
                     /gene="pliG"
                     /locus_tag="ACHKJF_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP0951347.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3513..3881)
                     /gene="ycgJ"
                     /locus_tag="ACHKJF_004085"
     CDS             complement(3513..3881)
                     /gene="ycgJ"
                     /locus_tag="ACHKJF_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP0951348.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            4401..5096
                     /gene="minC"
                     /locus_tag="ACHKJF_004086"
     CDS             4401..5096
                     /gene="minC"
                     /locus_tag="ACHKJF_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP0951349.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5120..5932
                     /gene="minD"
                     /locus_tag="ACHKJF_004087"
     CDS             5120..5932
                     /gene="minD"
                     /locus_tag="ACHKJF_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP0951350.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5936..6202
                     /gene="minE"
                     /locus_tag="ACHKJF_004088"
     CDS             5936..6202
                     /gene="minE"
                     /locus_tag="ACHKJF_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP0951351.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6574..6918)
                     /locus_tag="ACHKJF_004089"
                     /pseudo
     CDS             complement(6574..6918)
                     /locus_tag="ACHKJF_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6953..7072)
                     /locus_tag="ACHKJF_004090"
     CDS             complement(6953..7072)
                     /locus_tag="ACHKJF_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0951352.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(7033..7218)
                     /gene="ymgJ"
                     /locus_tag="ACHKJF_004091"
     CDS             complement(7033..7218)
                     /gene="ymgJ"
                     /locus_tag="ACHKJF_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP0951353.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7319..7492
                     /gene="ymgI"
                     /locus_tag="ACHKJF_004092"
     CDS             7319..7492
                     /gene="ymgI"
                     /locus_tag="ACHKJF_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP0951354.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7494..7838
                     /gene="ymgG"
                     /locus_tag="ACHKJF_004093"
     CDS             7494..7838
                     /gene="ymgG"
                     /locus_tag="ACHKJF_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP0951355.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7842..8177
                     /gene="ymgD"
                     /locus_tag="ACHKJF_004094"
     CDS             7842..8177
                     /gene="ymgD"
                     /locus_tag="ACHKJF_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP0951356.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8234..10555)
                     /locus_tag="ACHKJF_004095"
     CDS             complement(8234..10555)
                     /locus_tag="ACHKJF_004095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0951357.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     GSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10672..10881)
                     /locus_tag="ACHKJF_004096"
     CDS             complement(10672..10881)
                     /locus_tag="ACHKJF_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951358.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11281..11499)
                     /gene="ymgF"
                     /locus_tag="ACHKJF_004097"
     CDS             complement(11281..11499)
                     /gene="ymgF"
                     /locus_tag="ACHKJF_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP0951359.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(11631..13154)
                     /gene="pdeG"
                     /locus_tag="ACHKJF_004098"
     CDS             complement(11631..13154)
                     /gene="pdeG"
                     /locus_tag="ACHKJF_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0951360.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13486..13734)
                     /gene="ymgC"
                     /locus_tag="ACHKJF_004099"
     CDS             complement(13486..13734)
                     /gene="ymgC"
                     /locus_tag="ACHKJF_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP0951361.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         3938 a         3187 c         2956 g         3672 t
ORIGIN      
        1 agtaaaattt attaatagtt gtaaaacagg agtttcatta caatttatat atttaaagag
       61 gcgaatgatt atgactgaaa tcgttgcaga taaaacggta gaagtagtta aaaacgcaat
      121 cgaaactgca gatggagcat tagatcttta taataaatat ctcgatcagg tcatcccctg
      181 gcagaccttt gatgaaacca taaaagagtt aagtcgcttt aaacaggagt attcacaggc
      241 agcctccgtt ttagtcggcg atattaaaac cttacttatg gatagccagg ataagtattt
      301 tgaagcaacc caaacagtgt atgaatggtg tggtgttgcg acgcaattgc tcgcagcgta
      361 tatttttcta tttgatgagt acaatgagaa gaaagcatcc gcccagaaag acattctcat
      421 taaggtactg gatgacggca tcacgaagct gaatgaagcg caaaaatccc tgctggtaag
      481 ctcacaaagt ttcaacaacg cttccgggaa actgctggcg ttagatagcc agttaaccaa
      541 tgatttttca gaaaaaagca gcttttccag tcacaggtag ataaaatcag gaaggaagcg
      601 tatgccggtg ccgcagccgg tgtcgtcgcc ggtccatttg gattaatcat ttcctattct
      661 attgctgcgg gcgtagttga agggaaactg attccagaat tgaagaacaa gttaaaatct
      721 gttcagaatt tctttaccac cctgtctaac acggttaaac aagcgaataa agatatcgat
      781 gccgccaaat tgaaattaac caccgaaata gccgccatcg gggagataaa aacggaaact
      841 gaaacaacca gattctatgt tgattatgat gatttaatgc tttctttgct aaaagaagcg
      901 gcaaaaaaaa tgattaacac ctgtaatgag tatcagaaaa gacacggtaa aaagacactc
      961 tttgaggtac ctgaagtctg ataagcgatt attctctcca tgtactcaag gtataaggtt
     1021 tatcacattt aaaaacacag acgtttttta aagattgaga gttacctgtt gataataagg
     1081 tatcgggtaa ctctcattat gacagagcgc tttcaaaaac agggctacga aatggcgagc
     1141 atccagacgt atattaagaa agcccccatc aacatctatt aaatttatca ctgcgcccag
     1201 tcaggtttat ttaagatatg atcctgccag tcaatcactt ctgattcttt cactgcgata
     1261 tgacgcacag agatacgttc accatgcatt gccgctttcg aaccagtaag tagcggatgc
     1321 cacgcaggta aatctttacc ttctgccagc aaacgataag cgcaggtcat tggcagccat
     1381 tcgaatgttg gcagattatc acgggttaat ttaatgcagt cgggttcaaa ctcaaaacga
     1441 cgttcgtagt tccgacattg acaggtttta atattgagct ggcgacaggc gacgttagtg
     1501 aagtagattt cgtcggtgtc ttcatccatc agtttatgca ggcaacactg accgcaacca
     1561 tcacacaacg actcccattc cgcatcgctc atttcgtcca gggttttact ttgccagaaa
     1621 ggtacatcgc tcatcaggtg ttccgccatt acgttaaaac gcaccttata accagtctgg
     1681 cacagcgatg caagttttgc cgccgctttc aggcggcaaa aagtattaca aaacgcgagt
     1741 tgtcaaagaa tgcccatcga aagtgactgt cagctcatca ccgctttgca acgggccgac
     1801 gccatcaggc gtgcctgtca acacaacgtc accggccttg agggtaaaaa acttgctcat
     1861 ataagcgatc agcggaatga ttttatggat catgtccgca gtcgtacctt gctggcgttg
     1921 ttcgccgttt acgctcaggc ccagcgttgt attttgcgga tcgccggtaa attccgccgc
     1981 gggaataaac ccggaaagcg gacaagagtt atcaaacgct ttagcctttt cccacggctg
     2041 cccggctttc ttcatttttc cctgaacatc acgcaatgtc agatcaagcg ccacgccgta
     2101 accggcaatg gctttgcgga catgctcttc cgtagcctga cgcagcgtcg cgccaatcaa
     2161 caccgccagt tcgacttcat gatgaactga accgaaatcg gatgggatcg ccaatggctg
     2221 ccgcagatcg cacagtgccg tttctggttt aataaacagc actggctctt cgggcactgc
     2281 gctgcccatc tctttaatat gtttggcata gttactgcca acacagacta ctttactcac
     2341 cggataatcc agcagcgcac cttgccagtt gtgatgttga tacataacgt tcccctcacc
     2401 ttatcgtgga tatgaaaacc cgcgccgaca aagataatgc caccgccgga tatcggttat
     2461 ttgttagtgt cgtctgtttt ctgccccatg acggaaagat gttgcttcag caaatcttcg
     2521 ggtggtggcg gtaactgcaa atagtaacct tgctcggtta atgcctgttt aaccttttca
     2581 atatcggcat tgaccagttt cttccgccca tccagcggca gaatcatcgc taactgaggc
     2641 tgaccaaaac ctttcatcag ttcctcagga acacgcgaaa aatcgtcttt tttttcgaca
     2701 tataaatagg tctggtcacg cttgctgctt cgataaatca cacaaaacat acttttactc
     2761 tttaagatgc caggttttgg cataagctaa catcaatgaa acccgcgcca caagccagct
     2821 agctgtagat ttctacggtt tgttgagtcc atgccccctt tataagtcat ccccccggca
     2881 aaaatgccgc gacgggattt taccgaacgc aggaaagcca ctggaattct cccgtaaggg
     2941 attgttatat tataacagtt catcgtactc attctgaaca ggagactacc aatgaaaatc
     3001 aagagcatca gtaaggctgt attgctgctt gcactgctaa cctccacgag ctttgctgcg
     3061 ggtaagaatg tcaatgttga gttcagaaaa ggccatagca gcgctcagta ctccggcgaa
     3121 ataaagggat acgattacga tacatatacc ttctatgcca aaaaaggtca gaaagtgcat
     3181 gtgagtatct ctaatgaagg cgctgatact tacctcttcg ggccaggaat cgatgattct
     3241 gttgatttat ccagatattc cccagaactc gatagtcacg gacaatactc actccctgct
     3301 tctggcaaat atgagctgcg agtgctgcaa acccgaaacg acgcccgtaa aaacaaaact
     3361 aaaaaatata acgtcgatat tcaaattaag taaatgtcaa ccgacgggga ctctccccgt
     3421 cataaacgtt aacgccccac tcctctctgt cgctcaagag gttaatgacg aatagcagac
     3481 aatgccgaat agaacataat gtaaacaagg catcactcac gacacataaa taacatcttt
     3541 gtggtggttt gattgatttt tccactccgc ttaccatcta caccaaaata acgatcatca
     3601 cgacataatt tttctttaac atcgcaaaat acaccgttag cgaatgtaaa ttcgctggga
     3661 tcgtagcctt gtaatgattg taagttttca gcggcttttt cgccgagata tttccttgtt
     3721 aactctgggg aaatgccatc tgagttcaca caaatatacg ggtcgcaaac aacgccgcac
     3781 gccggagatt gaagtccgcc agcgtcattt ccagacgcca ttgatgagaa catcatcccc
     3841 acacctgaca atccgatata aaaaatacgc atcatattca tagcacatcc tgataatcca
     3901 accgtacaac acttatgctc atttcatcat caatccgtaa tcaaaaccgc tgacctggac
     3961 gagttacgat gacctgacag aagtatcaga cgtcttctgc ggtttctatg atagagcaat
     4021 tctgtggcgg atattaagat aacgggtatc tgttctttaa tgatgccgca aaaaatggaa
     4081 agttaagaaa gcctggcgtg gtgaaaaaat acacttgcct caatactgga tatttaaaca
     4141 ggtctggaga tgctgtcacg attagggtgc ggaaaaagga agcgtaaata agaagaatca
     4201 aaagtacgac ggcaatgggt tgattgacaa gggtattttt taagctatga atcagcgcca
     4261 tttatcacag aatagacttt tactctgaat aaatgggagg gtgacttgcc tcaatataat
     4321 ccagactata acatgcctta tagtcttcgg aacatcatcg cgcgctggcg atgattaata
     4381 gctaattgag taaggccagg atgtcaaaca cgccaatcga gcttaaaggc agtagcttca
     4441 ctttatctgt ggttcatctg catgaggcag aacctaaggt tatccatcag gcgctggaag
     4501 acaaaatcgc tcaggccccc gcatttttaa aacatgcccc cgttgtactc aacgtcagtg
     4561 cactggaaga cccggtaaac tggtcagcga tgcataaggc ggtttcggca accggtttgc
     4621 gggttattgg cgtaagtggc tgcaaagatg cgcaacttaa agccgaaatt gaaaaaatgg
     4681 ggctgcctat cctgacggaa ggaaaggaaa aagcgccgcg tccagctccc gcaccacagg
     4741 ctccagcgca aaatacaacg ccggtcacaa aaacgcgttt aatagatacc ccggtgcgtt
     4801 ccggtcagcg tatttatgct ccacaatgtg atctgattgt tacaagccac gttagcgctg
     4861 gggccgaatt gattgccgat gggaacattc atgtctatgg catgatgcgc ggtcgtgcgc
     4921 ttgcaggggc aagtggtgac cgggaaacgc aaatattttg tacgaacctg atggcggaac
     4981 tggtgtccat cgcaggtgaa tactggctga gtgatcaaat cccagcagaa ttttatggca
     5041 aagcggcgcg actgcagtta gtcgaaaacg ctttgaccgt tcaaccgtta aattgatccc
     5101 tttttaacaa ggaatttcta tggcacgcat tattgttgtt acttcgggca aagggggtgt
     5161 tggtaagaca acctccagcg cggccatcgc cactggtttg gcccagaagg gaaagaaaac
     5221 tgtcgtgata gattttgata tcggcctgcg taatctcgac ctgattatgg gttgtgaacg
     5281 ccgggtcgtt tacgatttcg tcaacgtcat tcagggcgat gcaacgctaa atcaggcgtt
     5341 aattaaagat aagcgtactg aaaatctcta tattcttccg gcatcgcaaa cacgcgataa
     5401 agatgccctc acccgtgaag gggtcgccaa agttcttgat gatctgaaag cgatggattt
     5461 tgaatttatc gtttgtgact ccccggcagg gattgaaacc ggtgcgttaa tggcactcta
     5521 ttttgcagac gaagccatta ttaccaccaa cccggaagtc tcctcagtac gcgactctga
     5581 ccgtatttta ggcattctgg cgtcgaaatc acgccgcgca gaaaatggcg aagagcctat
     5641 taaagagcac ctgctgttaa cgcgctataa cccaggccgc gtaagcagag gtgacatgct
     5701 gagcatggaa gatgtgctgg agatcctgcg catcaaactc gtcggcgtga tcccagagga
     5761 tcaatcagta ttgcgcgcct ctaaccaggg tgaaccggtc attctcgaca ttaacgccga
     5821 tgcgggtaaa gcctacgcag ataccgtaga acgtctgttg ggagaagaac gtcctttccg
     5881 cttcattgaa gaagagaaga aaggcttcct caaacgcttg ttcggaggat aagttatggc
     5941 attactcgat ttctttctct cgcggaagaa aaacacagcc aacattgcaa aagaacggct
     6001 gcagattatt gttgctgagc gccgtcgcag cgatgcagaa ccgcattatc tgccgcagtt
     6061 gcgtaaagat attcttgagg tcatttgtaa atacgtacaa attgatcctg agatggtaac
     6121 cgtacagctt gagcaaaaag atggcgatat ttctattctt gagctgaacg tgaccttacc
     6181 ggaagcagaa gagctgaaat aagcccgctg taaaaacgca tttatcttca aggcagagtc
     6241 atttctgcct tgagtttttc atccctctca tccacgttgt ggtaaagcgg cgagtattct
     6301 tgctgatact cctcattgct atttccacct ccccctttac accttaaggc tgttcccacg
     6361 cacagaacaa aatgtttatg cctggttgag taaataaccc tgttgttagt gtggatatac
     6421 tgggagatga taaccccgca atttcattat tcattgttct cccataggat gaggtttccc
     6481 gccgtaactg gcgggctttt tttgccccag aattttctct ttcaacatcc tgtaaacgaa
     6541 aactgcgccg aagcgcagtt taatcgttga tggtcagaac gaccagttca caccagccac
     6601 cgcgttccac ggggactcca caccggcacc atggctatac cccaccccga aatgcccgct
     6661 taacgtactg ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa
     6721 atcattgaca aaattaccgt cactattcac tttcacccga ttatcatcga caaattcttt
     6781 gcgcacagcc gccttcaggc agggttcaat ttccataccg ttccccagac gcatgttgta
     6841 actcagcgtt gcacccagtt cacgatatat actgcgggta tcgactgatt tcgattccat
     6901 gccattggat aaatgatagt agcgtctaag tgacctaagc tctgctgcaa catcagcccg
     6961 caggcaccag accaggggat tcatcctgaa gagacagcgc aggtgtattg tgttcaccgc
     7021 tcaccaaaga catcatgatg aaatgatgat atcccgcata agaaagaggc attttttaca
     7081 cgcagtgcgc tgaagtgtag ttggataaaa aatcactctt ttcagataat actgtcgtac
     7141 gcttatcttt cgacaatgag gccgtcggca aaataaaatg actaatgtaa tcgttgctga
     7201 tgaatatctt ctgctcacat aaaaatcaca caataatatt gataaatcag attatcttac
     7261 ctttacaaca ttgcgttaac agtcaatgac acttcttgaa taaaaatatt gtcaatcaat
     7321 gagttattcc tcatttaaga ttattctcat tcacgtaaaa aacataattc ctatcatcac
     7381 agccacgctc attttgaatt atctgaataa tagcgagcgt tctttagtta agcagatctt
     7441 aattgaagac gaaattattg tgtgtgcaac ttacttaata ccggacatct aagttgaaga
     7501 aaaaaatact tgccttcggg cttatctcag cattgttctg ctccacgcca gcgatggcgg
     7561 atatgaatcg caccacgaaa ggtgcattgc taggcgcagg cgttggttta cttactggca
     7621 atggtgttaa tggcgtactg aaaggtgcag ctgttggcgc tggtgttggt gcagtaacag
     7681 aaaaaggccg cgacggtaaa aatgcgcgta aaggtgcaaa agtaggtgcc gccgttggtg
     7741 ctgtcactgg cgttctgacc ggcaatggcc tcgaaggggc gattaaaggt gccgtgattg
     7801 gcggtacggg tggtgcaatt ctgggtaaaa tgaaataagg aatgatgatg aaaaaatttg
     7861 ctttactggc gggcctgttt gttttcgcac caatgacctg ggcgcaggac tacaatatta
     7921 aaaacggttt gccgtcagaa acctatatta cttgcgcaga agccaacgaa atggcaaaaa
     7981 ctgacagcgc acaggttgca gaaattgttg cggtaatggg taatgccagc gttgccagcc
     8041 gtgatttaaa aattgagcaa tctccggaac tgagcgccaa agtggtagaa aaactgaatc
     8101 aggtttgcgc taaagacccg caaatgctgt tgatcacggc aattgatgac accatgcgtg
     8161 ctatcggcaa aaaataatat ttcatatgta ccgcgtcgat gaatacgacg cggtttttta
     8221 gctaacggcg ggatcagaac ttatatttca ggcccaccat cactgcggtg tcattgtagc
     8281 cattatcacc cagctgcacg cccacattcc cccagacgct ggccgctgga ttcaggttgc
     8341 catttacgcc aagtttaatt tcccccaaat tactcgcccc atcttgttcg actttcacac
     8401 cgttcattga gacagcaaaa tctttactgt tatggatcca gttcatttca acaaaaggtt
     8461 caaagtcgtc accgtgcgga ccgctgtttt tctcctgagt acgaataaag gtgcggaaac
     8521 caagtcgggt ctggatgttg ttatttgcgt cattctcaat acgtgagcca ttggcttcgg
     8581 tgtgcgcatt atgatcgacg cccatccaga tagcctgtgc ctgtggctgg aatatccacg
     8641 tgtatttcgc cgcttccaga ccaaaggatt tattcagggc aaagccgtaa cccgcttcca
     8701 gcgagacggt tgcgcctttc gcactatagg attcataggc taattcatcc cctttcaccg
     8761 agtttttaaa ccagctatat tgcgcccagg cgtcaatgta tgcgcctttt ttactgatgt
     8821 catcggcaaa ccaggtggca tacagccctg cgcaatagcc tctgacactc cctttcgaac
     8881 gataatccga cacgctggaa tgagttgagt tgtagtcgcg ggcataacca gccatcactc
     8941 ccaaacgcca actgtcactg tcggtaaaac caccggttaa cagatcggcc cctaactgcg
     9001 agacgtagcg atgcgaggtc gttctcaact gtccgttgct gtcacgccag gcattatgcc
     9061 cgccaatttg acgtagccaa agtcggcttg aacgctcctg ttcagttaca ggatcgatgt
     9121 agcgcatttc acccgcacgg tcgttcagac gcataacaaa aagagtgtta gcggctgcca
     9181 ggttggcgac gtaacttcca gcttcgaggc gcaggacggg ggtaggcgca acagtaggcg
     9241 ctgaacttcc tccggagttt gtttcaccgc cgctcgttgc ctggaagtta gtcagatacc
     9301 agtttttatt ggtcgcttcg gtattacctc gggttaatga atattcgtag gcaccggcga
     9361 aaatcctcga atccttaata aattccccat ttgattcccc ctcaacgctg ataatctcta
     9421 tcccatttaa cgtgtaagct ccgctgcctc ctgcattggt aactttaact cgggttgttc
     9481 cagaggtatt tccattaata acgactttat ccgtagctga attatcatca cccaattcag
     9541 ttctgagatg caatacgcca ttattaccaa cgtaattttc agttattgtt aatcttgttg
     9601 gttcgacatc ccgtccatct gcccgggaaa tatagaccgt actattatca accgtgaggt
     9661 tgctcaccgt tgagtcagct ttaatatccc aggtactatt atctgaaagc gacagatagg
     9721 tatgagtgtt aacatctgtt gaaatattag ctgaacccgt tatttttgaa ttagttgcat
     9781 cgacggtcaa atgaccttta atattattga taaaaacatc tgcagccgat gatacctcac
     9841 tatccgtaat ggtcacgtta ctcgtctcat ttttatggta gaggatttca tttgctgacc
     9901 ctagtgagct accagataaa atatctaccg ttgcttcctg accatttatg gaagactcca
     9961 gcacaattcc agacccctga tgcgcgactg cagatgtatt tttcagcgtc accgtgccgc
    10021 cattataaat tttagcagtc gcggcgtcag gacccgttgt ataaatttct ccctggttaa
    10081 atgaaactgt cccctggcgg gcagcgatgg catgagcatt gtccccatgc gtggtgatat
    10141 caacatcggt taatgtaacg ctggcattgg tataatcaag cgtgtcacca gccttgacga
    10201 cagtgccatt agcgtacgcg gcataacttt ctgttccgtg ggttgtgatc tccccttttg
    10261 ccatatccag ttttgctatg gcattatttt cattacttcc tgtatcggta gtactttctt
    10321 catcaagaga agatacacac gcacgcgcgc ttccgggaga tgcacacgca agaacgccga
    10381 ctgcatgatt acccgcagta acaataaatg tatcatctcc gtcagaaaaa gagggggtaa
    10441 ccgaaccgtc agagttgact cttattatgg tgtcagtatt caggtttcca ctcgtttgac
    10501 ttagcaaacc aattgagtaa tcccccgtta tgacaatcgt gtttttaccc tgcaaaataa
    10561 cagcaccgcc aaattcactt acgattgcgc tagctgttaa tgctgtgctt agaggactat
    10621 tgtcagaagg atataaaccg cccgttgtta caatgacgcc attgttaatg gttaacttgc
    10681 taccctctcc tgcaacggat acccgtaact tccattaccc gttgttataa ctagcgcatt
    10741 atcaatatct tttacttttc catcttttat tgccacagca tgtttatatt catcgtgctg
    10801 aataaactgt tcagcatatg cattacccac cgccagagca ataagtccca aactaaaccc
    10861 ggagagtttt ttcagtttca tagattaacc atccattcat tggattaaca aacattcctg
    10921 gtcaaataac ctatgcgttc agattaccaa aacggagggt aaagacaaaa acaaaagcac
    10981 aaaaaattta aatagataaa cagcgacaat atataaatga taaataagtc ggcattatca
    11041 tttttcacct tattttcatg acattgatca ctttgaggtg acgattttgt ttaacaaata
    11101 actcttcgac atcacaatca ttgatagttg caataaaaat tatttcaatg acatataaaa
    11161 acaactgtga taacaccagg gttgacaact atttgcgcaa taattccttt acttcaatga
    11221 tccaggcatt cactgccagc aacccattca gccgcatact gacagtgaga aactgaaaga
    11281 ttattgctga tccctgtggc tgacgagtct ttcccttaat cgattaacca gattgatcgc
    11341 cagccaaaca agggcattaa gcaaatttat cgtcacaagc acttctccca gcccggtact
    11401 gggttttgca ataaggatga ccaggagggt gatcagcata aatgcaatgg aaaagatgat
    11461 atagagagtg aaataatcca gattattact attgttcatg ctactaatgg caatcctgtc
    11521 aaaactttca aacaattata gcaaacaaaa taatgaacaa tagtaacaag aggcaacgag
    11581 ctaattagct gtctataatt acaccgcact atggccttac tgatcaattt tcactcaacc
    11641 acaaccttca ccttcggttt agaaaggata attttgacca ggtcgatgta tgtaacaggt
    11701 ttatagaaat aataaccctg ctgaaatgtg atattatttt ggttcagata gtcaagttgt
    11761 tctttcgttt cgacaccttc agcgacgata ctgatcgaaa gtttacgcgc cagatccaat
    11821 acacaatcca gaattcgggt tgattctggg tctgcgttta cgcggccaac gaaactgtga
    11881 tcaattttga tataatcaat atgcaggtca tgaagataag aaagccctga gtagccagta
    11941 ccgaaatcat ccagcgcgat gacaaaacca ttttcatgca gaatgttcaa ccgctgaacc
    12001 agactttcat caacattcaa tggctcacgc tcggtgactt ccagaacaag gtttaaatcg
    12061 cggcgggtaa aactgtcacg gaaatttaaa cactcgtcga caaacgtcgg cgaaataata
    12121 tgcgaagcgc taaaattaat tccaatatga aaaccttccg gcaatttact cgcgatagcg
    12181 ttcatctgtc tggcaacctg attcatcagg ctttgcgtaa gcggaacgat taatccggat
    12241 ttttcagcaa gtggaataaa tgccgcgggt gatatatatc caccgtgagg ttgtttccag
    12301 cgggctaaca cctcaactcc ccgcaatgtc ccttcccgac catttaccac aggttggtaa
    12361 aaagggacga tctccccatt atttattgcc cgtcgcagga tctcttcagg cgttgcgctt
    12421 ttattgaaat atctatcaag caggaaggca gcggcgcagg cgattaacag aataaatatt
    12481 aaaatcccaa agcctttatc gataagtctg tagaagctaa agagtggcgg cgggttaaag
    12541 ataatagtga aatgatattt tgtggagtga accttctcgg tatagggata atgtccatta
    12601 agcgtcatca catcacccgt tggtccaata atgtcatccg ccacgcgtaa tacatacctt
    12661 acccctttca agggtacatt aagtgcccca cgaatatgct gatcgcttat cgtaaccaaa
    12721 atgcgcgtat ctgcaaattg gttctgatag agcaggatag gtaatctatt aacggtgtcg
    12781 attgctggag ccaacagtaa attactgtcc gggaaaacag gaatacgaga caacaggacc
    12841 ctattcccag gcagggatgt acaccagact attccctgtt tgagaatgat gattgtgcgc
    12901 aggtgaggtt tcagagctgc ttcagtgcca agctgatatt gctcctcgag gttgcattcc
    12961 ttcccggcaa tgttaaccgc tgtttgagtc gcttgtgacg cttcttcaag tatatgattg
    13021 atattgttgg cggcatatct cgctcccgcc aggtactctg cttttgcgga ataccagagc
    13081 tggatgttca aaatagcgac accggtaata aacaagcata tcgcaacgag gatgggtatg
    13141 agtgtattgc gcatttgctt gtcccggcca aagggtttaa tacatgaaaa tgccattcat
    13201 tcgctcgcgc tggcatctac agagagcatg gtagagagca taagaaatat ccacgccgaa
    13261 gataaaattt tctccatctt gatctaaccc attaattatt gagcataatg taggcagtac
    13321 aaaataagtt aggcgggata tcaggcgtca agaatggaga gcgagaactc tccattcttg
    13381 acacctgata ttgcggacat aataagaaag cataacgcct gaaatgctca ctttgcatca
    13441 gcatggtgat acagctgatg tttattctaa aaccttactc aagttctaag agagcacgga
    13501 ttccctgtca ttaataatga tatccgttag tgtagtctta tgcatttcaa tgtactgcaa
    13561 tatttgcaga tagagatcag aaagaatcac actggtttca tcttttaaac gcaagcgtaa
    13621 agcctgatca ataatatgac tatcaacata cccatgcgtc atgaacacct ccctctcgag
    13681 atttttaaac aaagcacatt gaaaaataaa tctctctggg attgaattat tcatgtcagc
    13741 ctcgtagaaa tat
//
LOCUS       DAZGXZ010000128        13708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000128 DAZGXZ010000000
VERSION     DAZGXZ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.128"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(44..757)
                     /gene="lpxT"
                     /locus_tag="ACHKJF_004100"
     CDS             complement(44..757)
                     /gene="lpxT"
                     /locus_tag="ACHKJF_004100"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP0951362.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(796..1782)
                     /gene="yeiR"
                     /locus_tag="ACHKJF_004101"
     CDS             complement(796..1782)
                     /gene="yeiR"
                     /locus_tag="ACHKJF_004101"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP0951363.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(1900..3366)
                     /locus_tag="ACHKJF_004102"
     CDS             complement(1900..3366)
                     /locus_tag="ACHKJF_004102"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0951364.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(3589..4161)
                     /gene="yeiP"
                     /locus_tag="ACHKJF_004103"
     CDS             complement(3589..4161)
                     /gene="yeiP"
                     /locus_tag="ACHKJF_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP0951365.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            4316..4570
                     /locus_tag="ACHKJF_004104"
     CDS             4316..4570
                     /locus_tag="ACHKJF_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0951366.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(4567..5748)
                     /gene="setB"
                     /locus_tag="ACHKJF_004105"
     CDS             complement(4567..5748)
                     /gene="setB"
                     /locus_tag="ACHKJF_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP0951367.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            6115..7245
                     /gene="fruB"
                     /locus_tag="ACHKJF_004106"
     CDS             6115..7245
                     /gene="fruB"
                     /locus_tag="ACHKJF_004106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP0951368.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            7245..8183
                     /gene="fruK"
                     /locus_tag="ACHKJF_004107"
     CDS             7245..8183
                     /gene="fruK"
                     /locus_tag="ACHKJF_004107"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP0951369.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            8200..9891
                     /gene="fruA"
                     /locus_tag="ACHKJF_004108"
     CDS             8200..9891
                     /gene="fruA"
                     /locus_tag="ACHKJF_004108"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP0951370.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            10315..11256
                     /locus_tag="ACHKJF_004109"
     CDS             10315..11256
                     /locus_tag="ACHKJF_004109"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP0951371.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            11244..12182
                     /gene="psuG"
                     /locus_tag="ACHKJF_004110"
     CDS             11244..12182
                     /gene="psuG"
                     /locus_tag="ACHKJF_004110"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP0951372.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELENTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            12276..13526
                     /locus_tag="ACHKJF_004111"
     CDS             12276..13526
                     /locus_tag="ACHKJF_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0951373.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT         3377 a         3331 c         3655 g         3345 t
ORIGIN      
        1 tcctcactat aaaaataagc ctgatgatgt taattactgt gagttatttg ttttggaaat
       61 gtttgttttt tcccggtagt gatttgtcaa aaaaggtgat taatcgatca cttaatggcg
      121 tcagcaacac ccagggcaaa ccgatcaata tcacggtcat cgaaccgaca atgatgtcag
      181 taaaccagtg tgcgccaatc attactctgg gaaatgcaaa aaccacaaaa ataataaggg
      241 cgataaggcc tgcaactttg ccgaaataac gccacatgaa tgccgaaaaa ataagcagca
      301 tcatgccgtg atcgccggga aagctatctc gtgaggcatc tttcgtggga acagagagca
      361 gttcgctgac gcggttaata tcggtaaaag tcaatgttgg gctggcccgt tttacaggaa
      421 ttaatgcctg acccagctgg tttaatacca ctgcagttaa tagcatgacc agacccataa
      481 tcacgatacg tcgtctgcca ggggcgtttt ctttcagcca gaaactcagc atcaacattc
      541 ccatcgccag cagagaacaa ccgtcgaaag cgcgattgtt ggtcaatgca accagccaca
      601 aaaaggcctt actttcgacc agtttctgat taaagaaata aaaaatatcc gcatcaattg
      661 gcaaccagaa tccatgatta acgggaatat accaggaaag aaacagcgcg aggccgacaa
      721 tattcaacaa cactatttgc ggcaaatttt taatcatatt ttctctaatt atcttactga
      781 aaaacgcagg caaccttacg cggtagtcgc taaacgaagc ttcaacagcg cgctctgcaa
      841 agcattccag tcagcttcgc tgctggaaat cagctcaata cggctgtccg gtggcgcaac
      901 gttttgcgtt tcaatgtgca ggtcatcgcc ctgacggttg attcgtacca gcccttcggg
      961 aatacgcagc acgcctttga cgcgttccac cggtgcaagt cgcgcccatt ccagaatgcc
     1021 aatggtgtcg aataccgtat cagcgtcgaa tatccagccg caggcctgat atccttgccc
     1081 gctgttcaga ctgcgacgcc agcgttgatg ctctggcagg cttaacgctg ctaacccttt
     1141 tttcacgaca tgctgatgag aatgcgcggc gctggcgggc aactcggcta aattgcgacg
     1201 cggcaaatcc agaagatgac cgtcaacttt cccatgctca ctgtgaatta attgtcgatc
     1261 gccaccattt tgctgccacc aacgctgtag cgcttgctca ctttcgggcg tcgcacggtc
     1321 ggatttattg gcgacaatga tgtctgcggc agccagctgg tcacggaagt tttcattgct
     1381 ggcgcttttt tcgtccagca gcaggcgtgg atcgagaatg cacaaggtgg cgcgcagatc
     1441 tatccacggt tcatagactg gtgcggttaa cagatcgagg atctgtttcg gatggcccag
     1501 cccggtcggc tctatcaaca agcggtctgg ttttccctga cgcagtaagg tattcaaccc
     1561 tacctgcatg ggtaaaccat taacgcagca catgcagccg ccggggatct ctttcagcaa
     1621 tgcgccgcta tcggcgagca aagcaccatc aattccgact tccccaaatt cattaaccag
     1681 taccgcccat ttttcgttgg gatctttatg ggctaacaga tgaagaatcg acgtggtttt
     1741 cccgctgccg agaaaaccgg tgatgaggtt ggtcctggtc acaacggctc cgcaatactc
     1801 tggtaagtta actgagcgtt aatcctggct aaaaaaggca gaaaagagaa ggggagagcg
     1861 gacgaaggtg agcttcatcc gctttaaccg cagaaatcgt taaattttga gggtgtttaa
     1921 tagcgcctga tgtgcgccga attgtacgat ttgttgccag gcttgttcga tggcctgcac
     1981 aaaatgcggg ttatctggca gatcatcacc gaaaacttca cgcagggaaa gcagggcggt
     2041 tacgcgttgt tcactgctgc tgcccgcaac aagttcgcga attttatcgc taagcggatc
     2101 gcgaacatca atggcatttc cggcatcatc aacgccgctg acgtaacgca tccagccagc
     2161 aacgcctaat gccagcaacg accagtccgt ttcgcgcccc agatgtatgc gaatacctgc
     2221 cagcatacgt tgcggtaatt tctggctacc atccattgcg atttgccagg tcttatgttt
     2281 cagcgccgga ttagcaaaac gtgcaattaa cttgtccgca tattgtgtta aatcgacatc
     2341 tttaatttgt agtgtcggcg cttgctcatt cagcattaat gttctggcgg catggcgaaa
     2401 tgcgcgatcc tgcatgcaat cactgatatg ggcgaatcct gaaaggtaac ccagataagc
     2461 gagaaaagag tggctgccat taagcatacg cagtttcatc tcttcccatg gcaggacatc
     2521 attcaccatt tgtacacctg cgacttccca ggcaggacgc ccagcgacga agttatcttc
     2581 caccacccac tggataaacg gttcgcagct aatcgcgcag ggatcattta cccccagatg
     2641 ctggctgatt tctgccagtg attcgtcggt tgcagccgga acaatgcggt cgaccatggt
     2701 tcccggaaaa ctgacgtgtt cttttatcca cccggcgagt tctggcgaac gtttttctgc
     2761 cattcccagc accgcgtttt tcaccacatg gccattgtcg ggaatattgt cgcaggagag
     2821 cacggtaaac ggtgtaaggc cgcgctcacg gcggcgtttc agtgcttcga cgagaatacc
     2881 cggtgcggag tgaggttctt caggggtttg tagatcgtga ataatccgcg gattactggt
     2941 gtcgagtgca ccggtggccg ggtcaataca atagcctttt tcggtaatcg tcagggaaac
     3001 aattgccacc tgtggctcgc aaaatttctc aataattgcc gctaaggaat ccagttttgc
     3061 attaaggcat tcgtgaacgg caccgacaat tatcacctga ttgccgtccg cacctttctc
     3121 cagcacggta tataaatggt tctgtgcgcg gagctggctc atcagttgat caccgctgaa
     3181 caagctgatt tcacagatcc cccagtcgcc gccctgggca ttcagcacac gatcggtcag
     3241 taatgcctga tgtgcgcggt gaaaggcgcc aaagccaaaa tgaacgatac gtgattgcaa
     3301 ctgctggcga tcgtagcgtg gagcgtgtac atgatgcggg agcgtaacgg aggcaattgt
     3361 cttcatagga gtcacgtctt agtaacgata aattaacaat gacagtgtaa agttatatga
     3421 cagcaattga aattggtctg gattgttaat gcgatgaagt gtgagggcga tcaatcaacg
     3481 agtgaaaatt gagggcgtaa ggtgttcaga aagcgcaaaa tagaagattg gtataataaa
     3541 ttaaaaaagg gacagcctga gctatccctt ttctgcatgc ggctgaagtt agtcagcacg
     3601 ccccatataa cggcgttctt cgatatggat acgaattttt tcgcccgggc ttaagtattc
     3661 tggtacctga atcaccagac cagtgctcaa tgtcgccggt ttgttacggg cgctggcaga
     3721 tgcccctttg atgcctggtg cggtttcaac gatttccaga tcaacggtct gcggcagctc
     3781 aagcgccagc agttgaccgt cccaggtcag cacctgcatg tccggcatgc cgccctctgg
     3841 cataaacagc aactcttctt caatctgatc tttggtgaag gtatacgggg tatagtcttc
     3901 tttatccata aagacatatt cgttgccatc gacataggag aagtcaacgt aacggcgggt
     3961 cagcgtcacg gtgtcaacga tatcatcacc tttgaaacgc tcttctactt tcagcccggt
     4021 acggacatca gaaaaacgca ttttgtacag cgtagcggca ccgcgggcag tgggcgactg
     4081 aatatcaata tccttaacca gcagcagttt gccgttgtaa ttcagtacca tacctttttt
     4141 aatttcgttc gctcttggca tcgaaaaatt cctgtagtcg aaaatgtcaa aaatatcgcg
     4201 acaaagtact cgcgacgggc ctttcgggca agtggtattc gcacttttgc tggtgcaaaa
     4261 aaggtggtac tgtgcgcgct catcaatccg gtggttaacc ttaagagaac aacgtatgga
     4321 atgccgtccg ggttgcgggg cgtgttgcac tgccccttca atttccagcc ctattcccgg
     4381 tatgccagat ggcaagcccg ccaatacgcc ctgcattcag cttgatgaac agcaacgctg
     4441 taaaattttc acctcgccac tgcgaccgaa agtctgtgca ggcttacaag ccagcgctga
     4501 aatgtgcggt aactcgcgtc agcaggccat gacctggctt atcgatctgg aaatgctgac
     4561 cgcgccttaa acatctttaa tccgcagtaa gcaaaacaga gtggcgataa tcatcaccat
     4621 cgcaaaccag aacacagcgt gataattcca gatctcggcg acgatgcccg ccactgatcc
     4681 tgcgatgatc cagcccacgc gcgaagtgtt ggtatagagc gtggtggctg aacccgcctg
     4741 accgggcatc agatcctgaa aatagagcat cccgatgccg cccagaatgc caataaaaat
     4801 agcatttagc agctgcaagc ccaacagaat gacaggtgaa tgcgccatca gcattcctgc
     4861 gtaaaaacag acgccaccca cggcagcaac gcgcattaag aaacgtttac ccagacgttt
     4921 ggcgaaatat ccggcaatca acatcgtcgg gatttccagc ccggcggcgg tccccatcat
     4981 cacaccggcc agtttctcgg gaagatgcag ttcgttgata ataaatagcg gcatgttgat
     5041 gatgtacagg ctgttcgagc cccacatcaa tgtacaaatg acaaacagca gtagcgtatc
     5101 gcgacggtta cggcgcggcg cttcaaccgt gccggtcgcc agcggaagct ctttttgcat
     5161 cgacggtaaa aacagccaca ccatcacgcc gcaaacaata aacgctaccg ctgcgctcag
     5221 atacattacc gtaaagctga aacccatcgc taacgcataa gccagcggtg ggccaatgac
     5281 ccatgccagt gaaacctgag cgcgtaaaaa agagctgaac atcaccgcct cacgtccggt
     5341 tttgtcggca tgttcacggg caagggcaaa catttgcggg ttagcggtcg agccaaagct
     5401 gctaagaaag acgccaacga atagcaaaac aaagtagttg cgattccagg caaaaagggt
     5461 gcaggccagc acgcctaaca ggcagcaaaa gacaatcagc gatttgcgat cgccgcgctt
     5521 atcagagcgc ccggcgagaa actgacttac cagaatccca atgacagcgc tgccggtaaa
     5581 gaagaatccc accatcgccg gacgggcatg tacttcatcg gtaagaaaaa tactgagtgt
     5641 cggggtttgc agagcgcccg caataccggt gagaaaggca acgattaaaa acgccgtcga
     5701 ggtcaggtca aacgatttcg cgctggagac tgcgggggag ttatgcatgc tggtcgatca
     5761 ctggaagagg aagacgcgga gtttacgctg cttgtcagaa aataaacaga cagcgaaatc
     5821 aaaagcgcca cgaataatca aaatgccatt tcactgttgc tgattggctg ctgacgctgc
     5881 tgaaatgaaa ttgctgataa cggattttcc catcagcaat taggaaaaat ggcaaaaaat
     5941 tgtgcagcac atcaaacttt tgctcataac tttacggctt tccttgcgtg ctgaaacaga
     6001 agtattatgc tttcttgaaa cgtttcagcg cgatcttgtc tttaacccta agccaggttg
     6061 gcgctttttt ctcatagagg ctgaatcgtt tcaattcagc aagagaggag aactatgttc
     6121 cagttatccg tacaggacat ccatccgggc gaaaaggccg gagacaaaga agaggcgatt
     6181 cgccaggtcg ctgcggcgct ggtgcaggcc ggtaatgtag cagaaggcta cgtcaatggc
     6241 atgctggcgc gcgaacagca aacctcaacg ttcctcggca atggtattgc tattccacac
     6301 ggcactaccg acacccgcga tcaggtgctg aaaaccggcg ttcaggtatt tcagttcccg
     6361 gaaggcgtca cctggggtga cggtcaggta gcgtacgtgg caatcggtat tgctgccagc
     6421 tcggatgaac atctgggcct gctacgccag ctgacccacg tactgagcga tgattccgtt
     6481 gctgaacaac tgaagtcagc aacaacagca gaagaacttc gcgcattact gatgggcgaa
     6541 aagcagagtg agcagctgaa gctcgacaac gaaatgctga cgctggatat cgtcgccagc
     6601 gatctgctga ctcttcaggc gctgaacgct gcgcgtctga aagaggcggg ggcagttgac
     6661 gccactttcg tcaccaaagc catcaatgaa caaccgctga atctcggaca gggtatctgg
     6721 ctgagcgata gcgccgaagg caatctgcgt agcgcgattg cggtaagccg tgcggcaaat
     6781 gcttttgatg tggacggcga aacggcagcc atgctggtga gtgtggcgat gaatgacgat
     6841 cagcccatcg cggttcttaa gcgtctcgct gatttattgc tcgacaataa agctgaccgc
     6901 ttgctgaaag cagatgcggc aacgttgctg gcgctgctga ccagcgatga tgcgccgacc
     6961 gacgacgtgt taagcgcgga gtttgtggtg cgcaatgaac acggcctgca tgctcgtcca
     7021 ggtaccatgc tggtcaatac cattaaacaa tttaacagtg atattaccgt gacaaacctt
     7081 gatggtaccg gcaaaccggc aaacggacgt agtctgatga aagttgtggc acttggcgtt
     7141 aagaaaggtc atcgcctacg ctttaccgcc cagggtgcag atgctgaaca ggcgctgaaa
     7201 gccattggcg acgctatcgc tgctggtctt ggggagggcg cataatgagc agacgtgttg
     7261 ctactatcac ccttaatccg gcttatgacc ttgttggttt ctgcccggaa attgaacgcg
     7321 gcgaagtgaa cctggtgaaa accaccggtc tgcatgcggc gggtaaaggc atcaacgtgg
     7381 ccaaagtatt aaaagacctg ggaattgatg tcaccgttgg cggcttcctg ggtaaagaca
     7441 atcaggatgg ttttcagcaa ctgttcagcg agctgggcat tgccaaccgt ttccaggttg
     7501 tacaggggcg cacccgaatt aacgttaagc tgacggaaaa agacggcgaa gtgaccgact
     7561 tcaacttctc gggttttgaa gtcacccccg ccgactggga acgctttgtg actgattctc
     7621 tgagctggct cggtcagttc gatatggtct gtgtcagcgg aagcttaccg tcaggcgtca
     7681 gcccggaagc gttcaccgac tggatgactc gcctgcgtag tcagtgtcct tgcattatct
     7741 ttgatagtag ccgtgaagcg ttagtagcag gtttgaaagc ggcaccgtgg ctggtgaaac
     7801 ctaaccgccg cgagttggaa atctgggcag gccgtaaact gcctgaaatg aaagatgtga
     7861 ttgaagctgc acatgcgcta cgtgaacaag gtattgcgca tgttgttatt tcactgggtg
     7921 ccgaaggcgc gctttgggtt aatgcctccg gcgaatggat cgccaaacca ccgtcagtcg
     7981 atgtcgtaag caccgttggc gcaggggatt ctatggttgg tggcctgatt tatggcttgc
     8041 tgatgcgtga atccagtgaa cacacactgc gtctggcgac agctgttgcc gccctggcgg
     8101 taagtcaaag caatgtgggt attaccgatc gtccgcagtt ggccgcaatg atggcgcgcg
     8161 tcgacttaca accttttaac tgacagcagg agaggcataa tgaaaacgct gctgattatt
     8221 gacgctaatc tcggtcaggc acgcgcctat atggcgaaga ccctgctggg cgcggcggcg
     8281 cgaaaagcaa aactggaaat catcgacaat ccgaacgacg cagaaatggc gattgttctc
     8341 ggtgattcca tcccgaatga cagcgcgctg aacggtaaaa atgtctggct gggtgatatt
     8401 tcccgggcag ttgcgcaccc tgagctgttc ctgagtgaag ccaaaggcca tgcgaaacct
     8461 tacactgcgc cggtcactgc gacagcaccg gttgccgcca gcggtccgaa acgcgtagtt
     8521 gcggtgactg cttgcccgac tggcgtagca cacaccttta tggcggctga agccattgaa
     8581 accgaagcga aaaaacgtgg ctggtgggtg aaagttgaaa cccgtggttc tgttggcgcg
     8641 ggtaatgcaa tcactcccga ggaagtagcc gcagcggatc tggtgattgt ggcggcagat
     8701 atcgaagtgg atctggcgaa atttgctggt aaaccgatgt atcgtacctc taccggtctg
     8761 gcgctgaaga aaaccgcgca ggaactggat aaagcggttg ctgaagcaac gccgtatgaa
     8821 ccggcgggca aagctcaaac ggcgaccact gaaggtaaga aagagagtgc gggcgcttac
     8881 cgtcacttgc tgacgggcgt ttcttacatg ctgccgatgg tcgttgctgg tggtctgtgt
     8941 atcgcgcttt cttttgcttt tggtatcgaa gcgtttaaag agccgggtac gttggcagcg
     9001 gcgctgatgc agattggcgg tggttcagcc tttgcgctga tggtgccggt actggcaggt
     9061 tatattgcct tctccattgc cgatcgtccg ggcctcacac cgggtctgat tggcggtatg
     9121 ctggctgtca gcaccggttc tggcttcatt ggcggtatta ttgcgggctt cctggctggt
     9181 tacattgcga agttaatcag tacgcaactg aaactgccac agagtatgga ggcgctgaaa
     9241 ccgatcctga tcattccgct aatttccagt ctggtggtcg gtctggcgat gatctacctg
     9301 atcggtaaac cagttgctgg cattctcgaa gggttgactc actggctgca gaccatgggg
     9361 actgcgaatg cggttctgct gggggcgatc ctcggtggca tgatgtgtac tgacatgggc
     9421 ggtccggtaa acaaagcagc gtacgcattc ggtgtgggtc tgctgagtac tcaaacctat
     9481 ggcccgatgg cggcgattat ggcggcaggt atggtgccac cgctggcaat gggtctggca
     9541 acaatggtgg cgcgtcgcaa attcgacaaa gcgcagcagg aaggtggcaa agccgctctg
     9601 gtattgggac tgtgcttcat ttcggaaggt gcaattccgt ttgctgctcg tgatccgatg
     9661 cgtgtgctgc cgtgctgtat cgttggtggg gcgctgactg gcgcaatctc aatggcgatt
     9721 ggtgcgaaac tgatggcacc acacggtggt ctgtttgttc tgctgatccc tggcgctatt
     9781 acgccggtac tgggttacct ggtagcaatt attgccggta cgctggtggc gggtttggcc
     9841 tatgccttcc tgaaacgtcc ggaagtggac gcagtagcga aagcagcgta ataaaaggtg
     9901 tgttacaggg cagaaatttc ctgccctgta tcattggatg agcgcaaagg gaatgaccag
     9961 cttgtgaatg taattttatg aatttaaatg gttttatctt tcagtagaaa cttgaaacgt
    10021 tcatcaaaca ctaaggttat ttcctcaagg gcagattatt ttctgccctt ttttattgtt
    10081 cctgttctgt gcatcccgtc acaaattcct cgcttttgta attcaggcat ttaatcgcat
    10141 tttgtgagcc tgctcaaatc tgcccatttt gcctctctgg cggtaattta ctttacgtaa
    10201 aacatgcata ctatgagcac atgtttaaat atgaacacat gtttaaaccg tctttgcagt
    10261 atctatattc ttgtggattg acagggttgg taatttttta ccagaggacg tgctatgcgc
    10321 gaaaaggatt atgtcgtaat tataggctcg gcgaatattg atgtcgccgg atattcacat
    10381 gaatcattaa attatgcgga ttcaaatcca ggtaaaataa aatttacgcc tggtggagtc
    10441 gggcgcaata ttgcacaaaa cctggcgttg ctggggaaca aagcctggct actgagcgcc
    10501 gtaggcagtg acttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc tggcgtttat
    10561 gtcgataaat gcctgattgt gccaggagaa aatacgtcaa gctatttatc attactcgat
    10621 aataccggtg aaatgctggt tgctataaat gacatgaata ttagcaacgc tattacagct
    10681 gaatatctcg cacagcaccg tgaatttatt cagagggcaa aggtcattgt cgcagactgt
    10741 aatatcagtg aagaggcact ggcatggatt ctggataatg ccgccaacgt acccgtattt
    10801 gtcgatccgg tttcagcgtg gaaatgtgtc aaagtgcgcg accgtctaaa tcagatccac
    10861 actctcaagc caaaccgcct tgaagcggaa accctgagtg ggattgcgct gtcagggcgt
    10921 gacgatgtgg caaaagttgc tgcctggttc catcaacatg gcctgaaccg actggtattg
    10981 agcatgggcg gcgacggcgt ttattacagc gatatcagcg gtgaaaatgg ctggtctgcg
    11041 ccgatcaaaa ccaatgttat taatgttacc ggagcgggcg atgccatgat ggcgggactt
    11101 gcttcgtgtt gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc acagggatgt
    11161 tcgtcaatgg cgctctcctg tgaatacacc aataaccccg atttatcgat tgccaacgtt
    11221 atatcgttag tggagaacgc agaatgtctg aattaaaaat ttcccctgaa ttattacaaa
    11281 tttccccgga agtgcaggac gctttaaaaa acaaaaaacc ggttgtggcg ctggaatcga
    11341 ccattatttc tcacgggatg ccgttcccac aaaatgccca gaccgcaatt gaagtagaag
    11401 aaactattcg taaacagggc gcagtacctg ccactatcgc cattattggc ggcgtgatga
    11461 aagtgggttt aagcaaagaa gaaattgaat tactgggtcg tgaagggcat aacgtgacca
    11521 aagttagtcg tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc gcaaccactg
    11581 tggcttcaac gatgattatt gcggcgcttg ccggaattaa agtatttgcc accgggggaa
    11641 ttggtggtgt gcatcgcggg gcggaacata ccttcgatat ttctgccgat ttgcaagaac
    11701 tggaaaatac taatgtcacc gttgtttgtg ccggggcgaa atctattctc gatttaggat
    11761 taaccactga gtatttagaa accttcggtg tgccgttaat tggctatcag actaaagcgc
    11821 tgcctgcgtt tttctgccgt accagctcgt ttgacgtcag cattcgtctc gacagcgcca
    11881 gtgaaattgc ccgtgcaatg gcggtgaaat ggcaaagcgg gctgaacggt ggcctcgtgg
    11941 tagcgaaccc gatcccggaa cagtttgcga tgccggagga atctatcaat gcagccatag
    12001 atcaagccgt cgccgaagcc gaagagcagg gcgttattgg taaagaaagt acaccgttcc
    12061 tgctggcgcg cgttgctgag ctgaccggcg gtgacagcct gaaatccaat atccagctgg
    12121 tgttcaacaa cgccattctg gcgagtgaaa ttgccaaaga ataccagcgt ctcgcgggtt
    12181 aatttctgac gggcagaaag ccgtcgcccg tactcatcaa gcataccaaa acctggcgct
    12241 gacgccgggt ttactggcat gaagggaaaa tcattatgga tataatgaga agtgttgtgg
    12301 ggatggtggt gttactggca atagcatttc tgttgtcagt gaataaaaag agcatcagtt
    12361 tgcgcacggt tggagccgca ctgctgctgc aaatcgctat tggtggcatc atgctctact
    12421 tcccaccggg aaagtgggca gtagaacagg cggcattagg cgttcataaa gtgatgtctt
    12481 acagtgatgc cggtagcgcc ttcatttttg gttcgctggt tgggccgaaa atggatgtcc
    12541 tgtttgacgg tgcgggtttt atcttcgcct ttcgcgtact tccggcgatt attttcgtta
    12601 ctgcgctcat cagtctgctg tactacattg gcgtgatggg gctgctgatt cgcatccttg
    12661 gcagcatttt ccagaaagct cttaacatca gcaaaatcga atcttttgtc gcagtcacaa
    12721 ctattttcct cgggcaaaat gagatcccgg cgatcgttaa accgtttatc gatcgcatga
    12781 atcgcaacga gttgtttacc gcaatttgta gcgggatggc gtccattgct ggttcgatga
    12841 tgattggtta tgccggaatg ggcgtaccaa ttgactacct gttagcggca tcgctgatgg
    12901 cgatcccagg tggtattttg tttgcacgta ttcttagccc ggccaccgag ccttcgcagg
    12961 tcacatttga aaatctgtcg ttcagcgaaa cgccgccaaa aagctttatc gaagcggcgg
    13021 cgagcggtgc gatgaccggg ctaaaaatcg ccgctggtgt ggcgacggtg gtaatggcgt
    13081 ttgtcgcaat tattgcgctg atcaacggca ttatcggcgg aattggcggc tggtttggtt
    13141 tcgccaatgc ctctctggaa agtatttttg gctatgtact ggcaccgctg gcgtggatta
    13201 tgggcgtgga ctggagtgat gccaaccttg cgggtagcct gattgggcag aaactggcga
    13261 ttaacgaatt cgtcgcttac ctgagtttct ccccatacct gcaaacgggc ggcacgctgg
    13321 aagtgaaaac cattgcgatt atctcctttg cgctttgtgg ttttgctaac tttggttcta
    13381 tcggtgttgt cgttggagcg ttttcggcta tttcgccaaa acgcgcgccg gaaatcgccc
    13441 agcttggttt acgggcgctg gcggcggcga cgctttccaa cctgatgagc gcgaccattg
    13501 ccgggttctt tattggttta gcttgagttt cattgccgga tgtactcgct tattaagcgt
    13561 tgcatccggc aatcgcgtac tatactcgtc atacttcaag ttgcatgtgc tgcggctgcg
    13621 ttcgctcacc ccagtcactt acttatgtaa gctcctgggg attcactcgc ttgccgcctt
    13681 cctgcaactc gaattattta gagtatat
//
LOCUS       DAZGXZ010000129        13553 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000129 DAZGXZ010000000
VERSION     DAZGXZ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.129"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKJF_004112"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKJF_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP0951374.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHKJF_004113"
     CDS             516..1217
                     /locus_tag="ACHKJF_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP0951375.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKJF_004114"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKJF_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP0951376.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHKJF_004115"
     CDS             complement(1616..2926)
                     /gene="cyuA"
                     /locus_tag="ACHKJF_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP0951377.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2954..4285)
                     /locus_tag="ACHKJF_004116"
     CDS             complement(2954..4285)
                     /locus_tag="ACHKJF_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0951378.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(4560..5924)
                     /gene="tdcG"
                     /locus_tag="ACHKJF_004117"
     CDS             complement(4560..5924)
                     /gene="tdcG"
                     /locus_tag="ACHKJF_004117"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0951379.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(5996..6385)
                     /locus_tag="ACHKJF_004118"
     CDS             complement(5996..6385)
                     /locus_tag="ACHKJF_004118"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP0951380.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFSTINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6399..8693)
                     /gene="tdcE"
                     /locus_tag="ACHKJF_004119"
     CDS             complement(6399..8693)
                     /gene="tdcE"
                     /locus_tag="ACHKJF_004119"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP0951381.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(8727..9935)
                     /gene="tdcD"
                     /locus_tag="ACHKJF_004120"
     CDS             complement(8727..9935)
                     /gene="tdcD"
                     /locus_tag="ACHKJF_004120"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP0951382.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(9961..11292)
                     /gene="tdcC"
                     /locus_tag="ACHKJF_004121"
     CDS             complement(9961..11292)
                     /gene="tdcC"
                     /locus_tag="ACHKJF_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP0951383.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11314..12303)
                     /gene="tdcB"
                     /locus_tag="ACHKJF_004122"
     CDS             complement(11314..12303)
                     /gene="tdcB"
                     /locus_tag="ACHKJF_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP0951384.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(12402..13340)
                     /gene="tdcA"
                     /locus_tag="ACHKJF_004123"
     CDS             complement(12402..13340)
                     /gene="tdcA"
                     /locus_tag="ACHKJF_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP0951385.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3553 a         3639 c         3168 g         3193 t
ORIGIN      
        1 cgcccccgcc agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc tcggtcacaa tggtgagatg
      121 cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgctcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccagac
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agcagagtat cgcgacagcg aaggcaatca tgttcaggcc agcgccggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctacag cgaacacaat ctcagcaaag
      841 acaaaccgtt aacacgaatg cagctttggc tggacgcctg cccgcagcga gagaatccgc
      901 tgattcaaaa actggcgctt aatatgggca agcagcaatt aatcgcctcg ccagagggta
      961 cgatgggaag cctgcaatta cgccagcaag tgtggctgca ccatatcgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacggga
     1081 aatttcatgc gcttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gttgccggat gcggcgaaaa cgccttatcc
     1441 ggcctacaaa atccaacaac attaacgaat taaacaaccc gtaggccgga taagatgcgc
     1501 cagcatcgca tccggcatca ggtacaaaac acaataacat caacaagttg agcaccccgt
     1561 aggccggata aggtgcgcca gcatcgcatc cggcacgata cccaaaacct ggcgtttatc
     1621 tggccttgct cgccataatc tcgataatct gccgatccgt ttgctgcatc gaatggcttg
     1681 ctaacgcaca caggttggca atcgactgct caacatcatg cgccacgatc ccttcattgc
     1741 cggtcacggc ggtatcatcc agcgccatta acaccgcttt ccacgcagcc gaagcactgg
     1801 tcgaaacctt catcgcgcag ctgttcgacg caccatcgca aatcatgccg ctgacatcgc
     1861 cgatcatact gctgatcgcc atcgagatgg tttcataacg cccatccacc agccatgcca
     1921 tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca cagcgcagac aaacgcggta
     1981 actggttatg gatgtaaatt gcgctcaaat gagaaagcat cagcgcacgc gccagccgtt
     2041 catcatccgc tccgaagtgt tctgctacca ccaccacagg cattgttgcg gtgatcccct
     2101 ggttacccga gccggagtta ctcatagccg gaagcgtagc gccgcccata cgcgcatcgg
     2161 atgccgcgct ggtacgaatc acaatggatg aagagagatc tttcgccagc aaaccgcgct
     2221 cgcactgttt ttccagcgtc gcgccaatat gcagccccca cttaccgctc aaaccttcct
     2281 gcgataacgc acaatttagc ttcgcggaat cgagaataaa gcggatcgcc gcaaacggga
     2341 cttcattgac gaacttcagg atctcagcca gcgtcgttct ggaaagcacc gtcagcggag
     2401 actcttgctc gccctctgcc acacacgcct gctgggtaaa caccacacca ttgtgcgtct
     2461 caatatgcac aatgttggta tgcccgccga cgatggtgac acacgcccac ttctcaccgt
     2521 tccagacttt ggcgcgtgag aagaggattt cattgcaagg ttcctggatc ttaacggaga
     2581 ctttccccgc cgccagcagt gctttggcat cggcaattgc ctgcgcagtt gcgtctttca
     2641 gcacttccag ccctgcgttg gcatttccac ctaacgcccc cagcgccgcc gcaatcggca
     2701 gccccaccat tcccgtgccg ggaacggtga cgcccagacc gttcttcatc agatttggcg
     2761 aaacccaggc ttctacacgt tcaaccggac cttccagttc tgccgcagca accgccgccg
     2821 ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg ttttacttcc tcctgaacgg
     2881 cgaggatgta acgctgccat aacggattta aagtcgaatc aaacatatta aaaaccttaa
     2941 aatttcaggt aaatcaagaa aatgccagga acggagaaac acaaagcaac aaaccagtga
     3001 cgataatcag gtacagagac atccctttgt atttgtgcaa tgccggtact ttgtaaacca
     3061 gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg gctacagata gaggtgaagc
     3121 tcaacaccgg tgcgttcagt acgatggcgc tccaggccag caaaatggcg aaaatcatga
     3181 tgccgcgctg aacaagattt tcgttaatct tctcggcagg catcttgcga cgcaggatgt
     3241 tcattacgat cccttgcgtt gcttcgcgaa agcctaaata gacgccaaag aatgcagtca
     3301 tgacagcaaa gatattgaga atgacgctga ccactttcac ccacgctgcg ccgtcaccgc
     3361 taataaactg tgcggcaatc gccagcgcgg aaatattctg ctcataggct tttaccgctt
     3421 cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa aaagacggtg acaaacaaaa
     3481 tgccaaacgc gatattcatt gcccgcaatg ctttatgccg cgccacttca attgattttt
     3541 cccgcgagcg ataagagatc accatcgggc ttaacgtctg gataaacaga atcgacgtca
     3601 gggtaaacgg cagcgtaata atggcgtttt tcaccagcag ccccagcggc ggtagcgaac
     3661 cgacgttata cagatgccac attcccacca tcgacacgcc cagcgccgcg accaccagca
     3721 gcttggtcag caccatgccg gtcgaaattt tgaataacaa tttctcgccg cgtgaagaga
     3781 tcgccaccag aatgcaaatc agtaccagac cataaaaggg actgtctgac agcaaccctt
     3841 ccgtcacgcc gaaggtatgc agataggaag cactatcgtt ggtgatggcg gtggaataga
     3901 cgaacatcca aatcaccagc attacgaaat agagcgcacc taacaggatg ccccagtttt
     3961 tacctaaata accgctaatg acactcgggt aatctttaca ttctggtgat tctgccagcg
     4021 tattaataaa caaccgctga aacagataca ttgccgggta accaatcacc gatgagagca
     4081 aaaataccca caatcccatc aaaccgacct gcaccgggag aaaaacaatc cccgcgccaa
     4141 tcgccatccc gatactcata atcacccagc cggtgtcagt actgtcgaat ttgatcgctt
     4201 ctcgccactc gctctcactc attccagcac gacccgccgg ggtcgaagcg tctgcaatga
     4261 cgcctttatt cgatgcaatt tccataattt ctcgctcaat attttgtagg gcttattatt
     4321 tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga gttattagaa
     4381 taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt tgaaataacg
     4441 gagatggata agaattttct actaaattaa tcgcaagaga atattcattc tctatttgcg
     4501 gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg atgaaagctg acagcaatgt
     4561 cagccgcaga ccactttaat ggccagtcct ccgcgtgatg tttcgcggta tttatcgttc
     4621 atatctttgc cggtttcata catcgtctcg atcactttat cgagtgaaac acgcggtgcc
     4681 gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt tcacggcatt aatggcatta
     4741 cgttcaatgc acgggatttg tacctgtccg gcaaccggat cgcaggtcag cccaaggtta
     4801 tgctccatcg cgatttccgc cgcattgcat acctgcgccg gactgccgcc cagtagttca
     4861 gttaaccctg ccgccgccat tgaacaggcc acgccaatct ccccctgaca gccgacctcc
     4921 gcgccagaga tggaggcgtt cattttatac agcgcgccaa tagcccccgc tgccagaaaa
     4981 tagcgggcaa ttgaccgctc gtttaccgga cgacggaact tatcgtaata agccagcact
     5041 gccggaataa tgccgcacgc accgttagtc ggtgccgtta ccacgcgccc gccagctgcg
     5101 ttttcttcac taaccgccag cgcgtacatg ttgatccagt cgatgacatt catcggatcg
     5161 ttagagatgt tatcgctgga aaccagctga cgacgcagcg ctacggcacg acgcggcaca
     5221 ttgagcggac caggcagcac gccttcagtg ttcatcccgc gttcaatacc gtcatgcatc
     5281 acttgccaga tacgggcaaa ccctgcgtca atttccgctt tgctgcgcag cgccagctcg
     5341 ttgtgcatca tcaggccaga tatagacagg ccgttgtaat cacacatttt cagcagttca
     5401 cctgctgagt ggaaatcgta aggtacggac gtttcgacat cgtgcgacag gccgaagtgt
     5461 tcttcttcga caataaaccc gccgccgaca gagtaatagg ttttacttaa tagcgcttcc
     5521 tgtcccttcc aggcagtgat ccgcattccg ttctcatgac gaggcaacat ttcgggatgg
     5581 aagataatgt tctttgctac aggaaaatca acaatatgcg caccagatgc cactggcagc
     5641 cgcccgctgc gcgttactaa ctctataaat gcagggatct catcaatgac aacatcctgc
     5701 ggactgtttc ctgccagtcc catgataatg gcgacatccg tggcatggcc tttgcccgtc
     5761 agtgacaacg acccatacag atcgaccaca atatggctcg tcgcggttaa taagccgcta
     5821 ctttccagcc gatcaataaa actttttccg gcattcattg gccccacggt atgcgaactg
     5881 gagggaccaa tcccaatttt gaaaatatcg aatgcactaa tcatatccac accctcggat
     5941 tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt cccgctcacg aggctttacg
     6001 cactacgtac tgcgatagct tcaatttcca gcttcacatc tttcggcaaa cgcgcaacct
     6061 gcacacagct ccgggtcggg taggtcgcct gatgctcatc gaagaactgc ttatagactt
     6121 cattgatggt ggaaaaatca ttcagatcgg tgatgaacac ggtcatcttg atgatatcgc
     6181 ccacgctcag cccggcagca accacgatcg ctttgacgtt ttcgaggctt aaacgcgcct
     6241 gatcttgcac atcagccggg atctcaccgg tctgcgggca aaccggaatt tgcccggagg
     6301 tgaagaccat gctgcctaaa tcaacgccct gaacataagg gccgattgcg cctggggcac
     6361 gttgcgtttc gataatcttt ttcatacatc ctcctgcgtc agagcgcctg ggtaaaagta
     6421 cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt tgaagcgcac ggcgtagcca
     6481 gagacacgga ttgtcaggtt aggatatttt tccgggtgct cgatggcatc cagcagcatt
     6541 tcccgattca ttacgttgac gttgaggtgt tgaccgcctt cgacatccgc ttcgtggtgg
     6601 aaatacccat ccagcaggcc gacaaggttg gttttacgta ctggatcttc tttgcccagc
     6661 gccgcaggaa cgattgagaa ggtgtacgag atcccatctt tggcgtaggt gaacggcagt
     6721 ttcgccaccg acgtcaatga ggccacggca cctttgcggt cacgaccatg catcgggtta
     6781 gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg gcgtattacc ggttttctgg
     6841 ccgtacacca cgttagaagt gatagtcaga atcgactggg tagggacggc gttgcgatag
     6901 gttggcagcg ctttaatttt cttcataaag cgttcaacca ggtcgcaggc aatgctgtct
     6961 acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt cgatttcaaa gtccaccgcc
     7021 aggccgtttt cgtcacggat tggtttcacg cgggcatatt tgatggcaga cagggagtcc
     7081 gtcgccaccg acaggcccgc gatgccgcat gccatagtgc gatagacatc acgatcgtgc
     7141 agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca tgtagtgaat gatattcagc
     7201 gcgctgatgt actgcaccgc cagccagtcc atgaagtgat cgaggctgtc catcactttg
     7261 tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg gcccgacctg aatcttcagc
     7321 ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt tcgccaggtt agcgcgtgca
     7381 ccaaagaact gcatttgctt accaatcacc attgggctga cgcagcaggc aatcgcgtaa
     7441 tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt catactgcaa ggaagaggtg
     7501 acgatcgaca cctgcgcggc atattttttg aaggcaatcg gtaattcttc cgaccaaaga
     7561 atggtcaggt taggttccgg tgccggcccc atagtgtgca gggtgtgcaa atagcggaag
     7621 gagtttttgg tcaccagcgt acgaccgtcc agccccatcc cgccgatcac ttccgtcgcc
     7681 cagattgggt cgccggagaa cagcgaatca aattccggtg tacgcaggaa gcgtaccata
     7741 cggatcttca tgatgaagtg atcgatcagt tcctgtgcct gctgctcatt gagtacgcca
     7801 gctttaaagt cgcgctcaat gtagatatcg aggaacgatg ccgtgcggcc cagcgacatc
     7861 gcgccgccat tttgcgattt cactgccgcc agataagcga agtagagcca ctgcaccgct
     7921 tcctgcgcat tctgcgccgg gcgagagata tcaaagccat atttcgccgc catttcctga
     7981 atctgcaaca gcgcatgacg atgctctgcc agctcctcac gcagacggat ggtggcttcc
     8041 agatcctcgc ctttttccag acgagactgg agatcggcaa attgcagttc gcgttcacgt
     8101 accagataac tgatgccata cagcgctacg cggcgatagt caccgataat gcgcccacgg
     8161 ccatagccat ctggtaaacc ggtcagcacg ccagatttac ggcagcgcag catatccggt
     8221 gagtaaacat caaatacgcc ctggttatgg gttttacgca gatcggtaaa cagatattca
     8281 aattcactgt ccatttcacg gccataggcg tggaatgaac ttttaatcat attaatgcca
     8341 ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt gcaggccaac aattttttcc
     8401 agcggctggt taatatatcc cgcatcatga gcggtaattg tggtggcaat attggtatcg
     8461 aaatcaaccg gcgcgtgggt tgcgttttcg atacggatac cttccattac tttttcccac
     8521 aattccgtgg tggcaggcgt cgcttcggcg aggaaagatt catcgccttc atacggtgta
     8581 tagttatgtt gaataaaatc gcggacatta atttcgtttt tccagtccgt acctttaaag
     8641 ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa tatctacctt catgaaaaat
     8701 aatctctcta caatacttca actaaattat gcaaattctg cgggcgcgtt aactttgcct
     8761 aaatgaatgg catccaaagc aatcattttt tcttcgttag tcggaataac agcgcaaatg
     8821 acacgcgcat tttcactgga aacaattcgc tcaccacagg agttagagcg attattcatt
     8881 tctgtatcaa tctctacgcc taatacagcc aaatgttcca tgaccagacg acgaattaag
     8941 cttgaattct ctcctattcc gccggtgaat ataattccat ccaggcgacg taatgaagct
     9001 gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg ttttaattgc cagttgcgcg
     9061 cgttcgtgac cttcatgcca ggctttttcc agaacacgta aatccgaaga aagaccggaa
     9121 atacctaata atcccgactc tttattcact acgcgttcca ggtcacccaa gctctggttg
     9181 gtttggctgg cgacccagga catcgcacca aaatcgacat cgccactgcg ggtacccatc
     9241 atcaagcctt ccagcggcgt cattcccatt gaggtgtcaa cactctgacc gttgcgaact
     9301 gcgcagattg acgcgccatt gccaagatgc gccacaacca ggccggagtc atcttccgcc
     9361 agattcagca aagaatgtgc gcgctgggaa acatagcggt gcgacgtgcc gtggaaacca
     9421 taacggcgta cacctaactc ttcataatat ttccacggca ggccgtataa ataagcttcc
     9481 ggagccatcg tctggtggaa actggtatca aataccgcca cctgagttac gcccggaaat
     9541 aattgctgcg ccgattcaat accacttaaa ttggcgtaat tatgcagggg tgccagtgga
     9601 gaaacgcgac ggatattatc aatgacctca tcggtaataa tggcggactc ggtaaaaata
     9661 ctgccgccgt gagcgatgcg gtggccaatt aaggccacac tgtcatttaa attccgtttt
     9721 tccagttcaa atgcaattgc cttcaatgca ccttcgtagc tgtggtgagc cagcggtgct
     9781 ggctctcccc catttacgga taagaatgca ttttccgagt taataccgtc ggcaatacct
     9841 gacattaata cttcacagtc gctggcatcg agcacggaaa acttaatcga agacgaacca
     9901 cagttaataa ccaaaacaac cggaaattca ttcatctctt ttctcatcct gagttacgga
     9961 ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga ccaatcacgg taacaaacac
    10021 gttatccaga cgaccacggt atttcgccag agacggcgct ttacggatgg catacatcgg
    10081 caacaggcac agcagggatg cgataatcgg tgcgcccatg gcttcaatca ggtcaaggat
    10141 gttcgggttg gcgtaggcaa caacccaggt ggagcccatg atgaagatca tgctgatagt
    10201 gttcagttta cccagcgaca ctttagtttt gtcgccttta taaccaaact tcaggaccag
    10261 gccattcaga ccttccagcg ttcccagata gtgaccgaag aaagatttga agatagccac
    10321 gagtgcgatg atggaagccg catattccag tgtaatcgcg aacgttgttt tggtaccggt
    10381 catggacgca aagtggttag ccagataaga aagcactgga atattctgcg ctttggcttc
    10441 cgccatgttg gccggagaca gcgtaaacag gcagctaaag gcaaagaaca tcaccactgc
    10501 aaccatcagc atgctggcac gagaaatgat ttgggaacat ttacgttcgg tgaagtcgcg
    10561 accgaagtct ttctcatact cttcacgttt agaaaccacg aaggaagaga cgattggcga
    10621 gaagttaaag gagaaaacca tgatggaaat ccccagccag acagtgatca ggataccgtc
    10681 atgaccggtt aacgacagcg aaccgaggtc aacctggtcg ataactgcag agttccagta
    10741 agggatcagc gacagagaaa tcagcaccag gctggcgata aacggccata ccaggtagct
    10801 catcacttta accatcagat ccttaccaaa ccagatgacg aaagccatca gcagcaacag
    10861 gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg agctggtttt cccagaacgt
    10921 cataaaggta ttggtaatag taacgccata aatccacagc agtgggcaaa tcgcgaagaa
    10981 gtacaggaac gtgataacca cgccgccagt tttaccaaaa tgctcttcca ccgtttccgt
    11041 aatgttgccg gaagggttag agccagaaag acacagacgc gccagcgccc ggtggcaata
    11101 aaatgcgatg gggtatgcca ataccaacat cagaagaatc gggatcagtc cgccaaaacc
    11161 tgcgcggata gggaagaaca gcaccccggc gccgattgcc gtaccaaaca agcctaacgt
    11221 ccatgtggta tctgatttac gccaggacga ttgttttgtc tggctggata caatgctatc
    11281 tgaagtactc atatcctatc ctcaacgaat taattaagcg tcaacgaaac cggtgatttg
    11341 agagacgcga gaaagatcga tattgccgcc ggaaataata ctgacggttt ttctgttttg
    11401 aatatattgg tctaatttac cgcttaataa tgcagcacat gccagagcgc ctgcgccttc
    11461 ggtgacgact ttattgcgct gaattaaggc aatcatactg tttctgattt cgtcttcgct
    11521 gaccagcacg atgtcatcga ctaattcacg aacgatttcg taagttaaat tacccgggcg
    11581 ggagacatca caaccatccg ccagggtgcc ggtagttcgg tgcgtggtta tttctccgga
    11641 gtggaaagaa gccgccatgc cgtgaacgtt ttcagactgt acgccaataa cacgaatggt
    11701 cgggttaata gatttaattg ccaccgcaat accagcaatt aaaccgccac caccaattgg
    11761 cacaatcacg ttatcgacat catagagatc ttccataatt tccagaccaa tcgttccctg
    11821 gccagcaatc actttcggat catcgtaagg tgggataaaa atacggcctt ccatttcgac
    11881 aatttcgctc actttagcga tagtgtcgtt gaaattatca ccatgcagaa cgacttctgc
    11941 ggagtagtcg cacgttgccg ctactttgga ttttggcgca ccttttggca tcaccacttt
    12001 accgtcgata cccagcatcg cgcaggagag cgaaacccct tgcgcatggt tgcccgcaga
    12061 acaggccact acacctttac gtttttccgc atcagtcagt gaacttaatt tattaaatgc
    12121 gccacgaatt ttaaatgaac ccgtacgctg catattttca aacttcagga atatttcacc
    12181 tttgcaacgt tcactaaaat agtttgagcg aggcatgcct gttttataaa ttcgcccagc
    12241 cagtcgttgt ttcgcttcaa taatatcatc aatagcaacc ggcagatcgt atgtaatatg
    12301 cattataaaa cctcttcgcc tgaattaaat atgtaggtaa ccgtaaccga cacctgcaaa
    12361 acagacaggt ggattattta tgtttaaata aaaacaaata actaaccaac ttctattaat
    12421 tgccttcgtc tacacccatt ataagatgaa tactctttgg ctaattccac caaaaccgat
    12481 gctgcttttt taatacgata atttttcgac cataccgcgg catattgtgc cacaggtaat
    12541 gtttcttcaa ccggaatagt aataaattga ttagaaccaa aaggtgacgt catatcacaa
    12601 ggaattacag ttaagaaatc agcattgaga acaagattat aaattgtcac gactgagtcg
    12661 gttttaacga tgttttcaat actgatgcca tttctttgta acgtagtaag cagttcgctg
    12721 tagtacccca tattagtttg tggcaacacc cactgttcgt tcttcaacga ctccagcgtg
    12781 gtggtgccgg tgcatgttcg ggacttactg gctaccagca caaactcgga ctcgaacagc
    12841 ggctcaacat gtaaatcctg aagcttcatt tctgcactta acgtcccaat cgcaaaatcc
    12901 agtcgaccgt cgcggattgc cggtaagaac gaagacagtt gcgcttcata catagaaacc
    12961 tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc ctgacataaa agtaaaacca
    13021 atcaatgaag gaaaaccaaa tgagacttcc accaccgcct cagaagacat accgcttatc
    13081 tcattaacca tatttttcat ttcacgggta atggattcgg aacgagagag taacagttga
    13141 ccggctggtg ttaatgttac accggtattt ttccgcacca ctaattccac accaaaataa
    13201 tcttcaatat cgttaatgat tttactgacg gccggttgag ttaaccctaa ttcttttgca
    13261 gccgagccga tagagccact tctaatgact tcctgaaaga ctaccaggtg ctgcgttttc
    13321 ggaagaagaa tagtgctcat aacgacctac gttaattacc tcattgacgg catgaagtgt
    13381 atcaaaatga aatgaacagg atatgtgcga ccactcacaa attaactttc aatactttcc
    13441 agggtatcgt tattacaatt aaataccttt aaatatcaac aagttaaagt ataaaaatca
    13501 gcataaaccc tgatttttgt caaaaaaaat gacggggata accatatttt atg
//
LOCUS       DAZGXZ010000130        13354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000130 DAZGXZ010000000
VERSION     DAZGXZ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.130"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..902
                     /gene="ydhF"
                     /locus_tag="ACHKJF_004124"
     CDS             6..902
                     /gene="ydhF"
                     /locus_tag="ACHKJF_004124"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP0951386.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            983..1504
                     /gene="sodC"
                     /locus_tag="ACHKJF_004125"
     CDS             983..1504
                     /gene="sodC"
                     /locus_tag="ACHKJF_004125"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0951387.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(1505..3517)
                     /gene="ydhK"
                     /locus_tag="ACHKJF_004126"
     CDS             complement(1505..3517)
                     /gene="ydhK"
                     /locus_tag="ACHKJF_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0951388.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3517..4374)
                     /gene="ydhJ"
                     /locus_tag="ACHKJF_004127"
     CDS             complement(3517..4374)
                     /gene="ydhJ"
                     /locus_tag="ACHKJF_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0951389.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(4377..4613)
                     /gene="ydhI"
                     /locus_tag="ACHKJF_004128"
     CDS             complement(4377..4613)
                     /gene="ydhI"
                     /locus_tag="ACHKJF_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP0951390.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            4814..5248
                     /gene="slyA"
                     /locus_tag="ACHKJF_004129"
     CDS             4814..5248
                     /gene="slyA"
                     /locus_tag="ACHKJF_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP0951391.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(5295..5762)
                     /gene="slyB"
                     /locus_tag="ACHKJF_004130"
     CDS             complement(5295..5762)
                     /gene="slyB"
                     /locus_tag="ACHKJF_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP0951392.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            6036..7145
                     /gene="anmK"
                     /locus_tag="ACHKJF_004131"
     CDS             6036..7145
                     /gene="anmK"
                     /locus_tag="ACHKJF_004131"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP0951393.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            7243..7572
                     /gene="mliC"
                     /locus_tag="ACHKJF_004132"
     CDS             7243..7572
                     /gene="mliC"
                     /locus_tag="ACHKJF_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP0951394.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            7631..8287
                     /gene="pdxH"
                     /locus_tag="ACHKJF_004133"
     CDS             7631..8287
                     /gene="pdxH"
                     /locus_tag="ACHKJF_004133"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP0951395.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            8416..9690
                     /gene="tyrS"
                     /locus_tag="ACHKJF_004134"
     CDS             8416..9690
                     /gene="tyrS"
                     /locus_tag="ACHKJF_004134"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP0951396.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            9749..10612
                     /gene="pdxY"
                     /locus_tag="ACHKJF_004135"
     CDS             9749..10612
                     /gene="pdxY"
                     /locus_tag="ACHKJF_004135"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP0951397.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(10656..11261)
                     /gene="gstA"
                     /locus_tag="ACHKJF_004136"
     CDS             complement(10656..11261)
                     /gene="gstA"
                     /locus_tag="ACHKJF_004136"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP0951398.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(11367..12869)
                     /gene="dtpA"
                     /locus_tag="ACHKJF_004137"
     CDS             complement(11367..12869)
                     /gene="dtpA"
                     /locus_tag="ACHKJF_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP0951399.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         3486 a         3300 c         3422 g         3146 t
ORIGIN      
        1 cgattatggt tcagcgtatt actattgcgc cgcaaggccc ggagttttcc cgttttgtga
       61 tgggctactg gcgattgatg gactggaata tgtccgcccg ccagctggtc agttttattg
      121 aagagcatct ggatctcggc gtgaccaccg tggaccatgc tgatatttat ggtggctatc
      181 agtgcgaagc ggcgtttggc gaggcactga aactggcacc tcacctgcgt gaacggatgg
      241 aaatcgtcag taaatgcggt atcgcgacga ccgcgcgtga agaaaacgtc attggtcatt
      301 acatcactga ccgcgatcac atcattaaga gcgccgaaca gtcgctaatt aatctcgcga
      361 ccgatcatct ggatctgctg ttaatccacc gaccagaccc gttaatggat gccgatgaag
      421 tggcggacgc gttcaaacat ctgcatcaga gcggcaaagt gcgtcatttt ggcgtatcga
      481 actttacgcc tgcgcaattt gccctgttgc aatcacgtct gccgtttacc cttgccacta
      541 atcaggtgga aatatccccg gtgcatcagc cgttactgct ggatggcacg ctcgaccaac
      601 tacaacaact gcgtgttcgt ccgatggcgt ggtcctgcct tggtggtggt cgtctgttta
      661 atgatgatta tttccagccg ctgcgtgatg aactggctgt ggtggcagag gagttaaacg
      721 cgggctcgat tgaacaggtg gtttacgcct gggtattacg tttaccatcg cagccgctgc
      781 caattatcgg ttcaggtaaa attgagcgcg tacgggcagc tgtcgaagca gaaacactga
      841 aaatgacccg tcaacaatgg tttcgtatcc gtaaagcggc actggggtac gacgtaccgt
      901 aagcactttt aggaatagcc gccgttcaaa aatgtgtcac tggtttacac ttattcagga
      961 atgcacaatg aacggaggtc ctatgaaacg ttttagtctg gctattctgg cgctggttgt
     1021 tgcaaccggc gcacaagctg ccagtgaaaa agtcgagatg aacctcgtca cgtcgcaagg
     1081 ggtagggcag tcaattggta gcgtcaccat tactgaaacc gataaaggtc tggagttttc
     1141 gcccgatctg aaagcattac cccccggtga acatggcttc catattcatg ccaaaggaag
     1201 ctgccagcca gccaccaaag atggcaaagc cagcgccgcg gaatccgcag gcgggcatct
     1261 tgatccacaa aataccggta aacatgaagg gccagaaggt gccgggcatt taggcgatct
     1321 gcctgcactg gtcgtcaata atgacggcaa agctaccgat gccgtcatcg cgcctcgtct
     1381 gaaatcactg gatgaaatca aagacaaagc gctgatggtc cacgttggcg gcgataatat
     1441 gtccgatcaa cctaaaccgc tgggcggtgg cggtgaacgc tatgcctgtg gtgtaattaa
     1501 gtaattacga ggccagcgta ccttgcggtg gtgcttgctc aagttgcgaa agcgagcagt
     1561 acagccgcca gacaattgcc gccagctcac gggcggcagg ttgatgatga cgggcaaggc
     1621 tgtcgcaaat ccgctgtaat tcttcaagtg tggccgccag tgatttttgc tgaacgccac
     1681 gctcactcat cactccccgc aacagtgaaa tacagttatc ccgtactcgc gataacggat
     1741 cggaacgcga ttcccagtcg cgcaattgcc agacaacatg agaacagttc agcagcacta
     1801 caccccagcg taataaccaa cggcgagcca gcgcatcctg gctgttactc aactgactga
     1861 cgtgatgata agtgagcgat tcaaattcgc tttcactcag tgttggatgg cggcttagct
     1921 gatcgacaaa atcccggcgc agcgcgcgaa tatggcggcg gcttttacga gcatccgatc
     1981 ctggacgcag aatggcgaac gctagccaag ccagcgcaac gccaacgatt tttgccagat
     2041 tatcgttaag aaaatcagca aaatcataca ccgggggatt agtgacagcg ataaacgaac
     2101 ccataaaaac aatcagttgc ccccacagtg cggcaaattt gggcatctgc aatttaagaa
     2161 gctgcattgt cgccagcagt ggaaagagaa acagtaaaaa ttgccacaga tcgctaatct
     2221 ggaccatcag gccaaatttg accacaaagc tgaatagcga aagtaacacc agcgtgcgca
     2281 tcagcagtga caacgactta aacggtgctg cgacggcgga gtagagtacg cagctaattg
     2341 ctgccagcgt taatgcattg gcaccggcat cccattgcga tgcaatactc catgcgccaa
     2401 tcatcatcaa cgtacaaaat gtacgcagcc cgctccacat agcttcggcg ttgtcggtat
     2461 gacgagccag agcggatgtc cgtgcgagtc tggcatgatc atctacaccg ctttgcagac
     2521 gatgtaattc ctgactactt tgcagataaa ggcggcaaaa ataacgtagt cgctgccaga
     2581 aggcgacgtg ccggtagtcg gcgacgttgg tcgggcgtag cggggcgata atacgtgcga
     2641 cggtgtaagc atctgtttgc gaactggcga gcgccgtcag caattgttcg agaatttctc
     2701 gcgtggcacc tggcggtgag ggccagttga gcaacatacg tcgcaggctg gagatgacac
     2761 tggtcatacg taattgctgg tggagtagcg cattaaggcg cgcgttttgc tggcgaaaac
     2821 gatagtggct ccagaaagcc tggatacgca gcaaattcat ggtcagtatc tgcccaatca
     2881 ccccctcatg tgctgcacga atggcatcgt ttgtttcagg ctgccagagt aaactggcat
     2941 gttccagtaa tcgggcgtgc atgtttttca atgcggttaa aagggctgta gcatcggaac
     3001 tgctaggcag gatcatcatc atcatgccgc cgcacaaaat accgactatc acctcgcaaa
     3061 cgcgcgcctg agcgatatcc cacagctggc tggcctcagt aatattaacc atcggaaagg
     3121 ctatgatggc agccgtgtag cccgccagtt gaaatgcata cgcgacgtta ttcgtgaagt
     3181 gcgcacaggc ccaggtacaa aagccaagcc acgccgacat gctcaataga aaaaaccacg
     3241 gctcattgag cgtatgcccg gcaagaagca gtgccgcaat ggctcccagt aaactgccag
     3301 cgatgcgtcc gaggcttttg ctgatgacac cgccaacggt gggaaagcta accactgcag
     3361 ccgaggtcat cgcccaatag ggttcatcca gattcaaata ataggcaacc gtcagcgcca
     3421 gacacatggc aatggtattg cgtaacgcat aacgccattg cgccagcgtg gccttgaacc
     3481 agggcaaatt gcgcaaggac catgacgatg cgttcattat tgacctacgg caatgctaca
     3541 ggtggtgccc gacaccagcg tgacatcgcc tggaacttta tccagcgcaa agcggacggg
     3601 aacgcgttgg gcgagacgaa cccaggggac gttaggtttc acatccggaa taaggcttga
     3661 gtcactttca acgctttgat cataaatcgc ccgaccgata ctggaaacgt gaccccgtaa
     3721 cgttttgtta tcactataga gggtaatttg tgctggtgca ccttcgcgga tatggcgcaa
     3781 cttggtttct tcaaaataac caatgacgta aaacgagtgg ctgtcgacaa gggcaaacag
     3841 cggtttcccg gtgtcagcgt aatcgccgat gcgggtagtg aggttagtca cccaaccact
     3901 caccggggcg cgaatttccg tttgcgccag ttgccattgt gtctgtttca gcgtggcctg
     3961 tgcggcatta acactggcct gcatcgcttt aacattgagg ttagcggtat cgagttcttc
     4021 ggcagaaata aaattttgtg agagatgacg acgacgattc gcttcgttat tggcttttgc
     4081 gaggtcagat tgcgctttcg ccagctgagc ctgtgcgttc agctcggcga tctgaaaggg
     4141 cgttttatcg atcgtgagca gaagatcccc cgcgttaacc agttggttgt cttttatatt
     4201 cagctcaaca atacgtcccg acacctgtgg ggtgatagaa accttttctg cgcgtatttt
     4261 cccgtcgcgt gtccacggag attgcatgta ataattccac agccaccatc cggcaagaac
     4321 cgcaactatc gctacaatga ttgttgagaa atacttaatt gttttaattg acatagttac
     4381 cacgcaatca gtattgccag agcaaggcaa acgcaaatcg caaacagcga taaatccatt
     4441 aacaagggat gccagatgtc accggcgtag atccagccac gaagcaggcg gtgtacgaca
     4501 agccagatga cgaatccaaa cgcgaaggct ttgaaaaacg gaggaaaata gacggacgca
     4561 ccgaacacca gatcttgtaa gggcaatcct gttgcattga gcataaactt cacaaagtag
     4621 attcctttac gaccgaatat attgcgttag attaataaat attctttaag tgcgaaaaat
     4681 ttacgcgcaa tttctgaaaa atatgtctga cggtaaccaa atgcagcaat acatttgttt
     4741 tagcaataca attgctgcac actattctaa aagccgcata atatcttagc aagctaatta
     4801 taaggagatg aaattggaat cgccactagg ttctgatctg gcacggttgg tgcgcatatg
     4861 gcgtgctctg atagaccatc gcctgaaacc gctggagtta acacaaaccc attgggttac
     4921 gttacacaat attcatcagt tacctccaga ccagtcgcaa attcaactgg caaaagcgat
     4981 tggcatcgag cagccatcac tggtccgtac tctggaccaa ctggaagaaa aagggttaat
     5041 ttcgcgtcaa acttgtgcca gcgatcgtcg ggctaaacgt attaaactga cggaaaaggc
     5101 agagccactg atcagcgaaa tggaagctgt tattaacaaa acccgcgcgg aaatattaca
     5161 tggcatctcc gcagaggaac tggagcaatt gattacgctc atcgcaaaac ttgagcataa
     5221 tatcattgag ttacaggcca aagggtgaaa tgaagggggc gtgtggtcag taacctgacc
     5281 acacgcaaac ttatttagcg cggagaaacg gtcacctgac tgccattgct ggccagtacg
     5341 acacgttggc ccggagagaa acgagtgttg ccttgtttct gtaccaccat gatggtatta
     5401 ccatcgtctt tacgaatttc cagctcgaca ccctgcgttt tgttcattgc actctgtacg
     5461 ccctgaccag ctacgccacc tgcaacagcg cctgctgcag tagccagaga acgcccggtt
     5521 ccgccaccaa cagtattccc caggaaacca ccaagaacag caccgccaat tgcaccgata
     5581 acgttggaat catcaccgcc ctgaatctgt accggacgta cgttaacgat ggtgccatag
     5641 ctgacattct gtacttgttt cgcttcagaa gcggtataaa catcccctga cagggtgtcg
     5701 ttattaacac aaccgacaag agacagacct accattgaaa caaccaatac gcgtttaatc
     5761 attgaaaaat ctcctgttca ccatgaaacg ccacgcaagc atccctcatg gtcaaagtat
     5821 atggcatatg tgaatccatg gttacatgtt ctaccaaata tgagcaaatc ataatgaaag
     5881 taggatcaac caattataaa caaaacattc atgatgatta ttgatttgaa gataatgttt
     5941 tactaccaac ggtaaaaatc ggtcattccg catggtattc acgaaaagtt tatgtttgag
     6001 tggtgcgcaa acgtcagatt gaaaaaggat gaactatgaa atcgggccgc tttattggcg
     6061 ttatgtcagg caccagcctt gatggtgttg atgttgtgtt ggcgacaata gatgaacacc
     6121 gggtcgcaca gctggcaagc ttgagctggc cgatcccggt gtctctgaaa caggctgtac
     6181 tggatatttg ccagggccag cagcttacac tttcgcagtt tggacagctt gatactcaac
     6241 tcgggcgact ttttgctgat gcggtcaatg ccttgcttaa ggaacaaaac ctgcaggcga
     6301 gagatatagt tgcgatcggc tgtcacggtc aaaccgtctg gcatgaaccg acgggcgtgg
     6361 caccacacac tttacagatt ggcgataaca atcaaattgt ggcacgcacc ggaattacgg
     6421 ttgtcggtga ttttcgccgt cgcgatattg ccttgggagg acaaggcgca ccgctggtac
     6481 ctgcgttcca tcatgccttg ctggctcact caaccgagcg acgaatggtg ctcaatattg
     6541 gcggcatcgc caatctgtca ctgctcattc ctgggcagcc ggttggcggc tacgataccg
     6601 gtcctggtaa catgctgatg gatgcctgga tctggcgtca ggccggtaaa ccttacgata
     6661 aagatgccga gtgggcacgg gcgggtaaag ttattctccc actgctgcaa aatatgctca
     6721 gcgacccata tttctcgcaa cctgcaccga aaagcaccgg acgcgaatac tttaactacg
     6781 gctggctgga gcgccattta cgccattttc cgggtgttga tccccgtgat gttcaggcca
     6841 cactggcaga actcaccgcc gtgaccattt ctgaacaagt tttgttgagc ggtggctgcg
     6901 aacgattgat ggtatgtggt ggaggtagtc gtaatccgct actcatggcg cgtctggcgg
     6961 cattactgcc aggcacagaa gtcaccacca ccgatgccgt tggcattagt ggcgatgaca
     7021 tggaagcatt ggctttcgcc tggcttgcct ggcggacgct ggcgggatta ccaggaaatc
     7081 tgccttccgt cactggcgca agccaggaga cggtactggg ggctattttc cctgctaacc
     7141 cgtgatatca gagttaactg aatttttctt accgcgtctg ccgttacact tggaacgtta
     7201 gggagggcgt attgccctcc agaccaggaa agtcttcggg aaatgtctat gaaaaaactg
     7261 ttactgatct gtttgccggt gcttctcacc ggctgtagcg cgttcaatca actggttgag
     7321 cacatgcaaa ccgctacgct ggaataccag tgtgatgaaa aaccgttgac ggtcaaactg
     7381 aataatccgc gccaggaagt cagttttgtt tacgataatc aactactgca tctcaaacag
     7441 ggcatttcag cgtctggtgc acgttacact gacggaatct atgttttctg gtcgaaaggc
     7501 gatgaagcga ctgtctataa acgcgaccgc atcgtattga ataactgtca gttacaaaat
     7561 ccacagcgtt gagatttttc caggggcggc gcacaatagc gtcacccact gacaatccgt
     7621 aaagaaaacc atgtctgata acgacgaatt gcagcaaatc gcgcatctgc gccgtgaata
     7681 caccaaaggc gggttacgcc gtcgcgatct tcccgccgat ccattaaccc tttttgaacg
     7741 ttggctctct caggcgtgtg aagccaaact ggcagaccct accgcgatgg tggtcgctac
     7801 cgtggatgaa catgctcagc cttatcaacg catcgtttta ctcaaacatt acgacgaaaa
     7861 aggcatggtg ttttacacca acctcggtag ccgtaaagca catcaaattg aaaataatcc
     7921 gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc caggtgatgg tgatcggtaa
     7981 agcagaacga ctttcgactc tcgaagtgat gaaatatttt catagccgcc cgcgtgatag
     8041 ccagattggt gcatgggttt cgaagcagtc cagtcgcatt tctgcccgcg gtatccttga
     8101 aagtaaattc ctggagctga agcagaagtt tcaacagggc gaagtgccat tgccgagctt
     8161 ttggggcggt tttcgcgtca gccttgaaca gattgagttc tggcagggcg gtgagcatcg
     8221 cctgcatgac cgctttttgt accagcgaga aaatgatgcg tggaagattg atcgtcttgc
     8281 accctgaaaa gatgcaaaaa tcttgcttta atcgctggta ctcctgattc tggcacttta
     8341 ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg gcaaaggtgc agtcgttata
     8401 tacatggaga ttttgatggc aagcagtaac ttgattaaac aattgcaaga gcgggggctg
     8461 gtagcccagg tgacggacga ggaagcgtta gcagagcgac tggcgcaagg cccgatcgcg
     8521 ctctattgcg gcttcgatcc taccgctgac agcttgcatt tggggcatct tgttccattg
     8581 ttatgcctga aacgcttcca gcaggcgggc cacaagccgg ttgcgctggt aggcggcgcg
     8641 acgggtctga ttggcgaccc gagcttcaaa gctgccgagc gtaagctgaa caccgaagaa
     8701 actgttcagg agtgggtgga caaaatccgt aagcaggttg ccccgttcct cgatttcgac
     8761 tgtggagaaa actctgctat cgcggcgaac aactatgact ggttcggcaa tatgaatgtg
     8821 ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta accagatgat caacaaagaa
     8881 gcggttaagc agcgtctcaa ccgtgaagat caggggattt cgttcactga gttttcctac
     8941 aacctgttgc agggttatga cttcgcctgt ctgaacaaac agtacggtgt ggtgctgcaa
     9001 attggtggtt ctgaccagtg gggtaacatc acttctggta tcgacctgac ccgtcgtctg
     9061 catcagaatc aggtgtttgg cctgaccgtt ccgctgatca ctaaagcaga tggcaccaaa
     9121 tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga agaaaaccag cccgtacaaa
     9181 ttctaccagt tctggatcaa cactgcggat gccgacgttt accgcttcct gaagttcttc
     9241 acctttatga gcattgaaga gatcaacgcc ctggaagaag aagataaaaa cagcggtaaa
     9301 gcaccgcgcg cccagtatgt actggcggag caggtgactc gtctggttca cggtgaagaa
     9361 ggtttacagg cggcaaaacg tattaccgaa tgcctgttca gcggttcttt gagtgcgctg
     9421 agtgaagcgg acttcgaaca gctggcgcag gacggcgtac cgatggttga gatggaaaag
     9481 ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc aaccttcccg tggtcaggcg
     9541 cgtaaaacta tcgcctccaa tgccatcacc attaacggtg aaaaacagtc cgatcctgaa
     9601 tacttcttta aagaagaaga tcgtctgttt ggtcgtttta ccttactgcg tcgcggcaaa
     9661 aagaattact gtctgatttg ctggaaataa tgcattaagt ggaaaggggg agtgagaaat
     9721 cactccccct ggtttttata cagggaacat gatgaaaaat attctcgcta tccagtctca
     9781 cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt cctatgcgcc gcctgggcgc
     9841 gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac acccaatacg gcaaatggac
     9901 tggctgcgtg atgccgccca gccatttaac cgaaattgtg caaggcattg ccgccattga
     9961 taaattacac acctgtgatg ccgtgttaag tggctatctg ggatcggcgg agcagggtga
    10021 acatatcctc ggtatcgtcc gtcaggtgaa agccgcgaat ccgcaggcga aatatttttg
    10081 cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt gcaccgggtg tcgcagagtt
    10141 tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg ccgaatctgg ttgagctgga
    10201 aatactctgt gagcatgcgg taaataacgt cgaagaagcg gttctggcag cgcgcgaact
    10261 cattgcgcaa gggccacaaa ttgtgttggt taaacacctg gcgcgagctg gctacagccg
    10321 tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagcc tggcatatca gccgtccgct
    10381 ggtggatttt ggtatgcgcc agccggtagg tgttggtgat gtgacgagcg gtttactgct
    10441 ggtgaaactg cttcaggggg caacgctgca ggaggcgctg gaacatgtga ccgctgcagt
    10501 ctacgaaatc atggtgacca ccaaagcaat gcaggaatat gagctgcaag tggtggctgc
    10561 tcaggatcgt attgccaaac cagaacatta cttcagcgca acaaagctct gaaatatctc
    10621 atttcggccc acaacgacct gtgggccgat ggatattact ttaagccttc cgctgacagc
    10681 gcgtcttgta cttccggacg ttcagccata cgttgcataa atgctgcaat gtgctctaac
    10741 ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa ccgtaaacag ataggcatca
    10801 gcaattgtaa atctttgccc gcagatccag tgctcatcct tcagtgcctc gttcacatat
    10861 tgcagcttct tctccagttg cgcgcgaact gtcggtttgt actcttccgg tgtatcaggg
    10921 cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga tgtaattcag ccattcgatg
    10981 gttttatagc gggaaatact gtttaccggt gccagcaact ggcggtcggg gacgctgtcg
    11041 gcaagatact gcataatcgc tacgccttcc gtcagcaaag taccgtcatc cagcagcaat
    11101 gcaggcacct gccccttagg gttaacggca aagtaatcgt caccgttttc gagacgtttt
    11161 ttcattaaat ccacactgac gagggtaaaa tcctttccgc tctcacgcag ggtgatatgg
    11221 gaagcgagag agcaggcacc cggtttgtag aacaatttca tcggtaactc cttttgtctg
    11281 tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa gccgctaacg aaaagttagc
    11341 ggcttgtaaa agagtttccc tgaattttac gctacagcag ctttcgccgc tttgtctgca
    11401 gcgtcatcct gcgtcatacg gtgcagttta ggcgcggtca gcagcatcag tactgcaata
    11461 atggcagtag cgacaccaat ctgcaagaat acgcgaccat aaacttccag tgacatcagc
    11521 gggtcggtaa cgttatccgg cacagccatc atacccgcaa cataaccacc aatcaggttt
    11581 gcaccggcag tggtcaggaa ccagctaccc ataatgaagc ccatcagacg ctgcggaacg
    11641 agttgtgcaa ccattgccag acccagacca gagatcatca gttccccgat gctctgcagg
    11701 ccatagcttg cgaccagcca gcttacagac acgataccag cgtcagacgc gaatttcgca
    11761 cccagcggca gaatcaggaa cgcaccagaa cacatcacca tgccgattgc aaacttggtt
    11821 ggcatcggca gggtatcgcc catcttgtta tagatagcgg ccagaatcgg actaccgatg
    11881 atgatccaga acgggttcag tgcctgatac tgctcaggtt ctacggccag acccagaatg
    11941 gagtgctcaa cgttacgaat cgcaaagaag ttcagtgacg ttggcatctg gctgtacagc
    12001 acgaagaaga taatggcttc gagcatcagg atgaaggcaa cgatcatttt acgacgcgca
    12061 gcacctttca tcgcgaaggc ttctttaccg aagataacca cgataccgaa ggcaacaacg
    12121 cccagcgcca tacgcgcaac ttcctgattg tgcagcagcc aggtggcgat agcgatcagt
    12181 gccacaacac caataatggt cagcagcagg ttacggtagt tgataggctc gaagtctggt
    12241 tttgaaccgt actgtttaac ccagcgttgg cagaaggcga agttaacgat agtgatcagc
    12301 aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt atttcgcggc cagccacggc
    12361 gtagcaatca tagagaagaa agagccgatg ttgacggaca tgtagtacat ggtgaatgca
    12421 ccgtccagac gcgggtcgtt tttctcatag catgtagaaa gcagagaaga cgggttagct
    12481 ttaaacaggc cgttaccgac cgcaatagcc gccataccca tataaacgat accggcgtcg
    12541 tgaccagacc aggcaaccag agcataacca atcgccagca caatagcgcc gagcataatt
    12601 acgcgtttag tacccagtac cttgtcacct aaccagccgc cgatagcgac cagaccataa
    12661 accagggcac taaaggaaga gaaaagggtg attgaatccg cttcagacat acccagttgt
    12721 ttaaccaggt aaacagccat aattccttgt aggccgtaat aaccaaaacg ttcccataac
    12781 tcaatcgaga agatgagata gaacgccttc ggttgtttga aagcgttcaa actgacgctt
    12841 tcagttggtt tttggtttgc agtggacact tttacctctt tttttacatc ccatattaac
    12901 gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc aattgttata gtgggagggg
    12961 aaacggcagg taatgttcac tatcttgcac tttcagacaa gtcctttgta ataatctgtt
    13021 acatataact gaggcgggga attcaaccat tccgggaaat gttatccagc gttaaatttt
    13081 tcaaaaaacg taacggcaga ttttttcact gcactgagag gggggtgatg gcttaacggc
    13141 gatatgttct gctatttagc gtttttacta gtgagatagt ccagtttctg aaaaatagcc
    13201 agtgtaatgt tttgtaggtc aatatttgtt gcgtgtgtaa atttaaatga ttttaacctg
    13261 attcttgcaa tgtttttaca ttgcgacagc atcgtgatct tgatcacgaa tgaatagaaa
    13321 atttcgctat taaaaaaaca attaacctgc attt
//
LOCUS       DAZGXZ010000131        13263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000131 DAZGXZ010000000
VERSION     DAZGXZ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.131"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            457..1170
                     /gene="aphA"
                     /locus_tag="ACHKJF_004138"
     CDS             457..1170
                     /gene="aphA"
                     /locus_tag="ACHKJF_004138"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP0951400.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1281..1697
                     /gene="yjbQ"
                     /locus_tag="ACHKJF_004139"
     CDS             1281..1697
                     /gene="yjbQ"
                     /locus_tag="ACHKJF_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP0951401.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1701..2057
                     /gene="yjbR"
                     /locus_tag="ACHKJF_004140"
     CDS             1701..2057
                     /gene="yjbR"
                     /locus_tag="ACHKJF_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0951402.1"
                     /translation="MIISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2092..4914)
                     /gene="uvrA"
                     /locus_tag="ACHKJF_004141"
     CDS             complement(2092..4914)
                     /gene="uvrA"
                     /locus_tag="ACHKJF_004141"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP0951403.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5168..5704
                     /gene="ssb1"
                     /locus_tag="ACHKJF_004142"
     CDS             5168..5704
                     /gene="ssb1"
                     /locus_tag="ACHKJF_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP0951404.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5803..6084)
                     /gene="yjcB"
                     /locus_tag="ACHKJF_004143"
     CDS             complement(5803..6084)
                     /gene="yjcB"
                     /locus_tag="ACHKJF_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP0951405.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6514..8100
                     /gene="pdeC"
                     /locus_tag="ACHKJF_004144"
     CDS             6514..8100
                     /gene="pdeC"
                     /locus_tag="ACHKJF_004144"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP0951406.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8103..8426)
                     /gene="soxS"
                     /locus_tag="ACHKJF_004145"
     CDS             complement(8103..8426)
                     /gene="soxS"
                     /locus_tag="ACHKJF_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP0951407.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8512..8976
                     /gene="soxR"
                     /locus_tag="ACHKJF_004146"
     CDS             8512..8976
                     /gene="soxR"
                     /locus_tag="ACHKJF_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP0951408.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9522..10871
                     /gene="ghxP"
                     /locus_tag="ACHKJF_004147"
                     /gene_synonym="yjcD"
     CDS             9522..10871
                     /gene="ghxP"
                     /locus_tag="ACHKJF_004147"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP0951409.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            11023..12672
                     /gene="yjcE"
                     /locus_tag="ACHKJF_004148"
     CDS             11023..12672
                     /gene="yjcE"
                     /locus_tag="ACHKJF_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP0951410.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12826..>13263)
                     /locus_tag="ACHKJF_004149"
     CDS             complement(12826..>13263)
                     /locus_tag="ACHKJF_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0951411.1"
                     /translation="HADLSRVNLNKAILQSINLVDSKLFCTWLTNTFLEMVICTGSNM
                     ANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDS
                     DTLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         3116 a         3240 c         3462 g         3445 t
ORIGIN      
        1 ctagctcaac aggtaaagat gtcgttttgt tgctattcac atatgatata ttcatatcat
       61 catctccatc gggagtaata aaattagtca ccattgtagg atagggttct ggtcaatcag
      121 gaatcatctt tataaacttc gattgttttt gtaatgctgt atcattaagt tcattaaatc
      181 gtacagcaga taaatgttct atcaaatttc gctcatttgc agaggattca tcataataaa
      241 cgtaaaatta atgtatcctt acatcgagta ataaacattt tttatacaaa aaaagacagg
      301 aacgtattta ctgggttaaa cataatcatc ctgcttttca tcacaaaaac cgcagataat
      361 ccttcctttc cccggcagct ggcgttatgg tcagatggtt tttgcaacaa atctcacaat
      421 aaaaagtttc aacatactga ctatttaggg aaaaatatgc gcaagatcac acaggcaatc
      481 agtgccgttt gcttattgtt cgctctaaac agttccgctg ttgccctggc ctcatctcct
      541 tcaccgctta accctgggac taacgttgcc aggcttgctg aacaggcacc aattcattgg
      601 gtttcggtcg cacaaattga aaatagcctc gcagggcgtc cgccaatggc ggtggggttt
      661 gatatcgatg acacggtact tttttccagc ccgggcttct ggcgcggcaa aaaaaccttc
      721 tcaccagaaa gcgaagatta tctgaaaaat cctgtgttct gggaaaaaat gaacaatggc
      781 tgggatgaat tcagcattcc aaaagaggtc gctcgccagc tgattgatat gcatgtacgc
      841 cgcggtgacg cgatcttctt tgtgactggt cgtagcccga cgaaaacaga aacggtttca
      901 aaaacgctgg cggataattt tcatattcct gtcaccaaca tgaatccggt gatctttgcg
      961 ggcgataaac cagggcaaaa tacaaaatcg caatggctgc aggataaaaa tatccgaatt
     1021 ttttatggcg attccgataa cgatattacc gccgcacgcg atgtcggcgc tcgtggtatc
     1081 cgcattctgc gcgcctccaa ctctacctac aaacccttgc cacaagcggg tgcgtttggt
     1141 gaagaggtga tcgtcaattc agaatactga cagagcggga gagcgtgacg ctctcccgaa
     1201 tgctgttttt ttaatcacac ctttatcctt tcgctgtctt gctgcaaact gattaagaga
     1261 gttttatcaa ggagcagcac atgtggtatc aaaagacgct cacgcttagc gccaaatctc
     1321 gtgggtttca tctggtaacg gatgaaattc tgaatcagct ggctgatatg ccgcgcgtta
     1381 acatcggctt actgcatctg ttgctgcaac atacctccgc ctctctgaca cttaatgaga
     1441 actgcgatcc caccgtacgc cacgacatgg agcgtttttt cctccgcacc gttcccgaca
     1501 acggaaatta tgagcatgac tatgagggag cagacgatat gccttctcat atcaaatcct
     1561 caatgctggg aacatcgctt gtattgccgg tacataaagg gcgtattcag accggcacct
     1621 ggcaaggcat ctggctgggg gaacatcgca tccacggcgg atcgcgtcgc atcatcgcga
     1681 cactacaagg ggagtaaaaa atgatcattt cggagttgct acaatattgc atggcaaaac
     1741 caggcgcaga acagagcgtg cataacgact ggaaagcgac gcagatcaaa gtggaagatg
     1801 tactgtttgc gatggtgaaa gaagtagaaa atcgcccagc tgtttcgctg aaaaccagcc
     1861 cggagctggc ggagctgcta cgtcagcagc acagcgatgt gcgtcctagc cgccatctga
     1921 ataaagcgca ctggagcacc gtgtatctcg acggttcgct gccggattcg caaatctatt
     1981 atctggtgga tgcgtcttat cagcaggcag tgaatttact accggaagaa aagcgtaaat
     2041 tgctggtaca actctgaaag gaaaaggccg ctcagaaagc ggccttaacg attacagcat
     2101 cggcttgagg aagcgtgccg tatgcgaagc ttcgcactcc gcgacggttt ctggcgtacc
     2161 ggagacgagg atttcgccgc cgccactgcc gccttctggt cccaggtcga caatccagtc
     2221 agcggttttg atcacgtcga gattgtgctc aatcaccaca atggtgttgc cctgatcgcg
     2281 cagtttatgc agtacgtcga gcagttgctg aatatcggcg aagtgcagac cggtggtcgg
     2341 ctcgtcgaga atatacagcg tctgcccggt gccgcgtttt gacagctcac gcgccagctt
     2401 cacgcgctgg gcttcaccac cagaaagtgt ggttgcggac tgccccaggc gaatgtacgt
     2461 caggccaacg tccatcaacg tttgcagctt acgcgccagt gcaggtacgg catcaaagaa
     2521 ctcacgcgcc tcttcgatgg tcatatccag cacttcgtgg atggttttgc ctttgtactt
     2581 aatttccagc gtttcacggt tatagcgttt acctttacac tgatcgcacg gcacgtagat
     2641 atccggcagg aagtgcatct ctactttgat cacaccgtcg ccctgacagg cttcgcagcg
     2701 cccgccacgg acgttaaagc tgaaacgtcc tggcgtataa ccacgcgcac gggattccgg
     2761 tacgcccgca aacagttcgc gcacaggcgt aaacacgccg gtataggtcg ccgggttaga
     2821 acgtggagta cgaccaattg ggctttggtc gatatcgatc actttatcga aatgctccag
     2881 cccctgaata tcgcgatacg gtgctggttc ggcgatggtc gccccattca actggcgttg
     2941 ggcaatcggg aacagtgtgt cgttaatcag cgtcgattta ccggaacctg aaaccccggt
     3001 gatgcaggta aacagaccca ccggcagcgt cagcgtcacg tccttcaggt tgttgccgcg
     3061 tgcgcctgtc agcttcagca ctttttccgg attcgccgga acgcgtttct tcggcacttc
     3121 aatcttgcgt ttgccgctca tgtactgccc ggtcaacgac tccggcaccg ccataatcgc
     3181 ttccagcgga ccttctgcga ccacttcacc gccgtgaaca cctgcgcccg ggccaatgtc
     3241 gatcacatgg tcagcggcgc gaattgcgtc ttcgtcgtgc tccaccacaa tcacggtatt
     3301 accgagatcg cgcagatgga taagcgtacc caacaggcgc tcgttatcgc gctggtgcag
     3361 gccgatagat ggctcgtcca gcacgtacat aacgccaacc aggcccgcac caatctggct
     3421 cgccagacgg atacgctggg cttcaccgcc ggaaagcgtt tctgccgagc gggaaagcgt
     3481 caggtaattc aggccgacgt taacgaggaa tttcaaacga tcgccgatct ctttaaggat
     3541 tttttccgca atcttcgccc gttgaccagc gagtttgaga ttgttgaaga attccatcgc
     3601 atgaccgatg ctcatgtcgg agatagcagg cagcggcgta ttctcgacat aaacgtggcg
     3661 cgcttcccga cgcagacgcg tcccttcgca gctggcgcat gggcgattgc tgataaactt
     3721 ggctaattct tcacgtaccg cactggattc tgtctcttta taacggcgct ccatattgtg
     3781 cagcacgcct tcgaacggat gacgacggat ggaggtatcg ccacgatcgt tcatgtattt
     3841 gaattcaatg ttttctttgc cagaaccgta caacaccact ttatgcacgt tcgcgctcag
     3901 gctgccccac ggcgcttcga cgtcgaactt atagtgatct gccagcgatt tcagcatctg
     3961 gaagtaatag aagttgcggc gatcccagcc acggatcgca ccgccagcca gtgacagctc
     4021 ggggttttgg atcacgcggt caggatcgaa atattgctgt acgccaaggc cgtcacaggt
     4081 cgggcaggca cctgccgggt tgttaaacga aaacagtcgc ggctccagtt cacgcatact
     4141 gtagccgcaa attgggcagg cgaagttggc ggagaacagc agctcttccg ctttcgggtc
     4201 gtccatatcc gccactaccg ctgtaccacc ggaaagctcc agcgcggttt caaacgactc
     4261 cgcaagacgt tgggtaagat cgtcacgcac cttgaagcga tcaaccacca cttcaatggt
     4321 atgtttcttt tgcagttcca gtttcggcgg atcggaaaga tcgcagactt cgccatcaat
     4381 acgagcacgg atgtaaccct ggcttgccag gttctccagc gttttggtgt gttcgccttt
     4441 gcgctcttta atgattggcg cgagcagcat cagacgcttg ccttccggct gcgacagcac
     4501 gttatccacc atctggctga cggtttgcgc cgccagcggg acgtcgtggt ccggacagcg
     4561 cggttcgccg acgcgggcga acaacaaacg caaatagtcg tggatttcgg tgattgtccc
     4621 caccgtagaa cgcgggttat gagacgtcga tttctgctca attgagatgg caggagaaag
     4681 cccctcaata tgatcgacgt ccggcttttc catcagtgac agaaactgcc gcgcgtaggc
     4741 ggaaagggat tcaacgtaac ggcgctgccc ttcggcatat aaggtgtcga aagcgagcga
     4801 ggatttgcca gaacccgaaa gcccggtcac gacaatgagc ttgtcgcggg ggataacgag
     4861 gttgatgttt ttgagattat gggtgcgggc gccccgaact tcgatcttat ccattcacct
     4921 ttcccggatt aaacgctttt ttgcccggtg gcatggtgct accggcgatc acaaacggtt
     4981 aattatgaca caaattgacc tgaatgaata tacagtattg gaatgcatta cccggagtgt
     5041 tgtgtaacaa tgtctggcca ggtttgtttc ccggaaccga ggtcacaaca tagtaaaagc
     5101 gctattggta atggtacaat cgcgcgttta cacttattca gaacgatttt tttcaggaga
     5161 cacgaacatg gccagcagag gcgtaaacaa ggttattctc gttggtaatc tgggtcagga
     5221 cccggaagta cgctacatgc caaatggtgg cgcagttgcc aacattacgc tggctacttc
     5281 cgaatcctgg cgtgataaag cgaccggcga gatgaaagaa cagactgaat ggcaccgcgt
     5341 tgtgctgttc ggcaaactgg cagaagtggc gagcgaatat ctgcgtaaag gttctcaggt
     5401 ttatatcgaa ggtcagctgc gtacccgtaa atggaccgat caatccggtc aggatcgcta
     5461 caccacagaa gtcgtggtga acgttggcgg caccatgcag atgctgggtg gtcgtcaggg
     5521 tggtggcgct ccggcaggtg gcaatatcgg tggtggtcag ccgcagggcg gttggggtca
     5581 gcctcagcag ccgcagggtg gcaatcagtt cagcggcggc gcgcagtctc gcccgcagca
     5641 gtccgctccg gcagcgccgt ctaacgagcc gccgatggac tttgatgatg acattccgtt
     5701 ctgatttgtc attaaaacaa taggttatat tgttttaagg tggatgatta aagcatctgc
     5761 cagccataaa aaagaagcct ccgttatgga ggcttctacg tatcaggtca aaatcattgg
     5821 ccattgtggc ggtgtctgat tcatctcttc tatcatcacc gactcgatgt tgtgccagat
     5881 agcggtgatg tccgccattg tgatgccaag caaccccatt gcgaaccaac aggcggaaac
     5941 aacgcccaga ccgctgctga tcaccgcaag cccgacataa tcagaccgac gaaaacggat
     6001 gttgagtgtg ctggccagaa acatcattac ggcactaaga agttgccagc gaagaagaac
     6061 cacgccagtg gtgagggtag ccatcaaaca attcctctga aaagagccga tgccctggac
     6121 agcgcggcgt tgttcacggg aggcgtggta cactctggct atcgcggggc ttgcagaaca
     6181 caaaaatgaa acactctgtt tgtttcatta attttgtgaa ctatatcaca attgattgtt
     6241 tgttagccat attaggccgt gacttttatt gctgtacaga ttatgtggtt tttcagtggc
     6301 attaagggca tatcttcccg ccgcctctgc attcctgtag gaaattaatt ttgaatatca
     6361 atgaattatt ttcatccagg tgacgattag aaaggtatca atttcaaatc aggcaaaagt
     6421 gctatttata ccgtaagatt tatctaaaga cgtcggtacc cagggttttc accttgcaat
     6481 ggccgggtat aaacaggcag gaaattgata gcaatgagtc atcgtgcacg acaccaatta
     6541 ctggcgttgc cgggcattat ctttttagtt ctctttccca tcattctttc gctatggatt
     6601 gccttccttt gggcaaaatc agaagtgaat aatcagctcc gaacctttgc tcaactggca
     6661 ctggataaat ccgagctggt cattcgccag gcagatttag tgagcgatgc agctgaacgc
     6721 tatcaggggc aagtttgcac tccagcccat caaaagcgaa tgttgaatat tattcgtggc
     6781 tatctttata ttaatgaatt gatctatgcc cgtgataacc attttttatg ctcatcgctg
     6841 atagcgcctg taaacggcta tacgattgca ccggccgatt ataagcgtga acctaacgtt
     6901 tctatctatt attaccgcga tacgcctttt ttctctggct ataaaatgac ctatatgcag
     6961 cggggaaatt atgtggcggt tatcaaccct ctcttctgga gtgaagtgat gtctgatgat
     7021 ccgacattgc aatggggtgt gtatgatacg gtgacgaaaa cctttttctc gttaagcaaa
     7081 gaggcctcgg cagcaacgtt ttctccgctg attcatttga aggatttaac cgtacaaaga
     7141 aatggctatt tatatgcgac agtttattcg acaaaacgcc caattgcagc cattgttgcg
     7201 acttcatatc aacgtcttat aacccatttt tataatcatc ttatttttgc gttgcccgcc
     7261 ggtattttgg ggagtcttgt tctgctatta ctctggctac gtattcgaca aaactattta
     7321 tctcccaaac gtaaattgca acgcgccctc gaaaaacatc aactttgtct ttattaccag
     7381 ccaataatcg atatcaaaac agaaaaatgt atcggcgctg aagcgttgtt acgttggctt
     7441 ggtgagcagg ggcaaataat gaatccggca gagtttattc cgctggcaga aaaggagggg
     7501 atgatagaac agataactga ttatgttatt gataatgtct tccgtgatct gggcgcgtac
     7561 ttggcaacac atgccgatcg ctatgtttct attaacctgt cggcctccga ttttcatacg
     7621 tcacggttga tagcgcgaat caatcagaaa acagagcaat acgcggtgcg tccgcagcaa
     7681 attaaatttg aagtgactga gcatgcattt cttgatgtcg aaaaaatgac gccgattatt
     7741 ctggctttcc gccaggctgg ttacgaagtg gcaattgatg attttggtat tggctactct
     7801 aacttgcata accttaaatc attgaatgtc gatattttga aaatcgacaa atcgtttgtt
     7861 gaaacgctga ccacccacaa aaccagtcat ttgattgcgg aacacatcat cgagctggcg
     7921 cacagcctgg ggttaaaaac gatcgctgaa ggcgtcgaaa ctgaggagca ggttaactgg
     7981 ctgcgcaaac gcggcgtgcg ctattgccag ggatggtttt ttgcgaaggc gatgccgccg
     8041 caggtgttta tgcaatggat ggagcaatta cccgcgcggg agttaacgcg cgggcaataa
     8101 aactacaggc ggtggcgata atcgctggga gtgcgatcaa actgccgacg gaaaacgcgg
     8161 gagaaggtct gctgcgagac ataacccagg tccattgcga tatcaaaaat cggacgctcg
     8221 gtggtgcgca actcaacggc ggccagtaac aggcgacgtt ggcgaatgta atcgccaagc
     8281 gtctgatgcg tcaccgtgcg gaacattcgt tgcaagtacc actttgaata gcctgatttt
     8341 tttgcgacta catcaatgtt aagcggctgg tcaatatgct cgtcaatcca tgcgataaga
     8401 tcctgaataa ttttctgatg ggacataaat ctgcctcttt tcagtgttca gttcgttaat
     8461 tcatctgttg gggagtataa ttcctcaagt taacttgagg taaagcgatt tatggaaaag
     8521 aaattacccc gcattaaagc gctgctaacc cccggcgaag tggcgaaacg cagcggtgtg
     8581 gcggtatcgg cgctgcattt ctatgaaagt aaagggttga ttaccagtat ccgtaacagc
     8641 ggcaatcagc ggcgatataa acgtgatgtg ttgcgatatg ttgcaattat caaaattgct
     8701 cagcgtattg gcattccgct ggcgaccatt ggtgaagcgt ttggcgtgtt gcccgaaggg
     8761 catacgttaa gtgcgaaaga gtggaaacag ctttcgtccc aatggcgaga agagttggat
     8821 cggcgcattc ataccttagt agcgctgcgt gacgaactgg acggatgtat tggttgtggc
     8881 tgcctttcgc gcagtgattg cccgttgcgt aacccgggcg accgcttagg agaagaaggt
     8941 accggcgcac gcttgctgga agatgaacaa aactaaagcg ccacaagggc gctttagttt
     9001 gttttccggt ctttgtcttt ctctctatcc cgctggtaca caggagggtt tcccccgacg
     9061 tcaacacacc ccattcgagc acgtggtgga ggttccggtt ggtgttgatg ctttaattgt
     9121 atgtcaccgg catttcttcg ccagtgtaaa agtatacttt ttatccgcaa tatttttgtc
     9181 atctcagacg attttttatc gcaatcctga acggtatacg gctcgataac gctgcaatct
     9241 tgcgcaccga cgataacgtt tgcgcatcaa ttgcctggtt tttcatcgtc aagacaataa
     9301 aagagaaaaa agcagcaaac ttcggttgaa aaagccgcta tgatcgccgg ataatcgttt
     9361 gcttttttta ccacccgttt tgtatgcgcg gagctaaacg tttgcttttt tgcgacgcag
     9421 taaattgtcg caaacctgga gcaggaagat aacgtttcgc tggcagggga ttgtccgcca
     9481 cgcatcttga cgaaaattaa actctcaggg gatgttttct tatgtctacg ccatcagcgc
     9541 gtaccggcgg ttcactcgac gcctggttta aaatttcaca acgtggaagc actgtccgtc
     9601 aggaagtggt tgccgggtta acaacgtttc tggcgatggt ctactcggtc atcgtcgttc
     9661 caggtatgtt gggtaaagca ggcttcccgc ctgcggcagt tttcgttgca acctgtctgg
     9721 ttgccggact cggttctatc gtgatgggtc tgtgggctaa tctgccgttg gcgattggtt
     9781 gcgccatctc cctgacggcg tttaccgcat tcagcctggt gctggggcaa catattagcg
     9841 tacctgtcgc gctgggtgcc gtgttcctga tgggggtgct gtttacggta atttctgcca
     9901 cgggtatccg tagctggatt ttgcgcaact tgccgcacgg tgtggcgcac ggcacgggga
     9961 tcggtatcgg tctgttcctg ctgctcattg ccgctaatgg tgtcggtctg gtgattaaaa
    10021 acccgcttga tggtctgccc gttgcgctgg gtgatttcgc gaccttcccg gtgattatgt
    10081 cactggtagg tctggcggtg atcatcggcc tggaaaaact gaaagtccct ggtggcattc
    10141 tgctgaccat tatcggtatc tcaattgtcg gtttgatctt cgatcctaac gtccatttct
    10201 ccggcgtttt cgccatgcct tcattgagcg atgaaaacgg caattcactg attggcagcc
    10261 tggacattat gggcgcgctg aatcctgtag tcctaccaag cgttctggcg ctggtgatga
    10321 cggcagtatt tgatgccacc ggaactatcc gtgccgtcgc cggccaggcg aacctgctgg
    10381 ataaagatgg gcagatcatc gacggtggga aagcactgac cactgactcc atgagcagcg
    10441 ttttctctgg cctggtgggt gctgctccgg cagcggtata catcgagtct gcggcgggta
    10501 cggcggcggg cggtaaaacc ggtttgacgg ctatcaccgt tggcgtgctg ttcctgctga
    10561 ttctgttcct ctctccgctc tcttacctcg ttccggggta tgcaacggct ccggcgctga
    10621 tgtacgttgg cctgctgatg ctgagcaacg tggcgaaaat cgactttgct gattttgttg
    10681 atgcgatggc gggtctggtt acggcggtat tcatcgtgct gacctgtaac atcgtaacag
    10741 gcatcatgat cggcttcgcg actctggtga ttggtcgtct ggtttccggc gaatggcgca
    10801 agttgaacat cggtacggtc gttatcgccg tggcgctggt gaccttctat gcgggtggct
    10861 gggctatcta atctttcatc ggattctgaa aacgggtggc aatggctgcc cgtttttatt
    10921 ttctccgcac attgtgtgag cttttgcgat attctgaaaa aatgagaatt caggcataac
    10981 gtctttctca gatcataaaa aaacatcgca aacagggaac gcatggaaat cttcttcacc
    11041 atactgataa tgaccctcgt ggtctcgctg tccggggtgg tcactcgtgt catgcccttt
    11101 cagatcccgc ttccgcttat gcaaatcgcc atcggtgcgc tactggcgtg gccgacgttt
    11161 ggtttgcatg tggagtttga tcctgaactc tttttagtct tgtttatccc gccgttgctg
    11221 ttcgctgatg gctggaaaac gccgacccgt gaatttctcg aacatggtcg agagattttc
    11281 ggcctcgcgc tggcgctggt ggtggtcacc gtggtcggca ttggcttcct tatttactgg
    11341 gtggtgccgg gcattccgct gatccccgcc tttgcgctgg cggcggtact ttctccgacc
    11401 gatgctgtgg cgctctccgg gattgttggc gaagggcgca tcccgaaaaa aatcatgggc
    11461 attttgcagg gcgaagcgtt gatgaatgac gcctccggcc tggtgtcgtt gaagtttgcc
    11521 gtggcagtgg cgatggggac gatgatcttc accgtcggcg gtgcgacggt cgaatttatg
    11581 aaagtagcca ttggtggtat tcttgccggt tttgtggtga gctggctgta tggtcgctcg
    11641 ctgcgattcc tcagccgctg gggcggtgat gaacccgcga cgcagatcgt tctgctgttc
    11701 ttgctgccat tcgcttctta tctgattgcc gaacatattg gcgtttccgg catcctcgct
    11761 gcggttgccg ccgggatgac catcacccgc tccggtgtga tgcgccgtgc gccgctggca
    11821 atgcgcctgc gcgcaaacag cacctgggcg atgctggaat ttgtctttaa cggcatggtg
    11881 ttcctgctgt taggtctgca gctgccgggt attctggaga cgtcgctgat ggcggcagaa
    11941 atcgacccta acgtcgaaat ctggatgctg tttaccgata ttattctgat atatgcggcg
    12001 ctgatgctgg tccgtttcgg ctggctgtgg acgatgaaaa agttcagcaa ccgcttcctg
    12061 aagaagaagc cgatggagtt tggttcgtgg accacacgag aaatcctgat cgcgtctttc
    12121 gccggggtgc gtggggcgat cactctggcc ggtgtgctct ctatcccgct gctcttgccg
    12181 gatggtaacg tcttcccggc gcgctatgag ctggtgttcc tggcggctgg cgtcattctc
    12241 ttctcgctgt ttgtcggcgt ggtgatgttg cctattctgc tacaacacat tgaagtcgcg
    12301 gaccattcgc aacaattgaa agaagaacgt attgcgcgag cggcaacggc agaagtggcg
    12361 attgtggcga tccagaaaat ggaggagcgt ctggcggcgg ataccgaaga gaatatcgat
    12421 aaccagctgc ttacggaggt cagttctcgc gtcattggta acctgcgtcg tcgcgccgat
    12481 ggacgtaacg acgttgaaag ttccgtgcag gaagagaacc ttgagcgtcg cttccgtctg
    12541 gcggcattgc gttctgaacg tgctgaactg taccacctgc gcgccacgcg ggagatcagc
    12601 aacgaaacgc tgcaaaaatt actgcacgat ctcgatttgc ttgaagcgtt gctaattgag
    12661 gaaaatcagt aagccgaaag cgacgtaacc aggcccgagg taatctgaaa aaggtgcaat
    12721 cttttgcacc tttaacaggt tagctcgggc taacattcat gattctaaaa caaataagtt
    12781 atctctcttg taaagtcaat tcattggctg ttcttcgact taacctcaat ctgcgtgttg
    12841 cataatatct gcagtaatct tcatagcgac tgcattaata ttatcctcag tttgtgacat
    12901 gccagtatga gaggtacaat cttcttcaaa gaatttctgt agtgttacca gggtatcact
    12961 atcaatgggg atattttcct ccccacggag aatagaaatg cccattccct ggacgctagc
    13021 ctcatcaaaa ttaacccgat agagacgggt attaaacata caggcttttg ttaaaataga
    13081 acagttgaaa tggcagttgc ttaaattggc attattaaaa ttaacattag ccatattaga
    13141 gccggtacat ataaccattt cgaggaatgt atttgttagc cacgtacaaa agagtttgct
    13201 atcaactaag tttatactct ggaggattgc tttgttcaga ttgacgcgag acagatcagc
    13261 atg
//
LOCUS       DAZGXZ010000132        13088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000132 DAZGXZ010000000
VERSION     DAZGXZ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.132"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            232..780
                     /locus_tag="ACHKJF_004150"
     CDS             232..780
                     /locus_tag="ACHKJF_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0951412.1"
                     /translation="MNVNKKNLAEMFNVDPRTIERWQTQGLPQISGGGKGIEIIFDSA
                     KAIEWYAQREADIENEKLRKEVEDLRAASETDLQPGTIDYERYRLTKAQADAQELKNA
                     RDSGQVIDTGFCMYALARLAQDISGILDSIPLYMQRQFPEFPPSALEFLKSEIAKASN
                     KCADTADGLPDMLADYIRESNS"
     gene            731..2680
                     /locus_tag="ACHKJF_004151"
     CDS             731..2680
                     /locus_tag="ACHKJF_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0951413.1"
                     /translation="MGYQICLQTTLESQIHKFVFPFRAGLKGMIRPLPVTPVEWADRH
                     YYLPPESSYLAGRWETLPFQMAIMNCMGNDRIRTVNLIKSARVGYTKMLLGVVGYFIE
                     HKSRNTLLFQPTDSSAEDFMKSHVESTLRDVPCLKILSPWLGRKHRDNTLTLKRFTSR
                     VGFWCLGGAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIR
                     GSTPKIKGTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPES
                     VFYLCEHHGCVIHQSEPDQSNGRWICENTGMWTRDGLTFFSAAGNEIPPPRSITFHIW
                     TAYSPFTTWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHY
                     TAAVPARVVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDA
                     AINKKYRHADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVIT
                     MPKTRNQRGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQ
                     QLVAEELVEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSR
                     EEETTRPTLKELAAKLSGGVNGYSR"
     gene            2664..2870
                     /locus_tag="ACHKJF_004152"
     CDS             2664..2870
                     /locus_tag="ACHKJF_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP0951414.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2867..4459
                     /locus_tag="ACHKJF_004153"
     CDS             2867..4459
                     /locus_tag="ACHKJF_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0951415.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDWQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQQDNPLINILEKFSNWYDTNHVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4449..5954
                     /locus_tag="ACHKJF_004154"
     CDS             4449..5954
                     /locus_tag="ACHKJF_004154"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0951416.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSLPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTSVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVCQDMQQRIDAAHRMFAEKVAMYTGLSVEAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASAQSEADRIMACEEAKGREQLAATLAAMPD
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5991..6338
                     /locus_tag="ACHKJF_004155"
     CDS             5991..6338
                     /locus_tag="ACHKJF_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0951417.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAVRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            6396..7424
                     /locus_tag="ACHKJF_004156"
     CDS             6396..7424
                     /locus_tag="ACHKJF_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0951418.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7476..7859
                     /gene="gpFI"
                     /locus_tag="ACHKJF_004157"
     CDS             7476..7859
                     /gene="gpFI"
                     /locus_tag="ACHKJF_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0951419.1"
                     /translation="MATKEENQKRLRELAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGSDQNYTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            7852..8205
                     /locus_tag="ACHKJF_004158"
     CDS             7852..8205
                     /locus_tag="ACHKJF_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0951420.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8221..8754
                     /locus_tag="ACHKJF_004159"
     CDS             8221..8754
                     /locus_tag="ACHKJF_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951421.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSASQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELASALKQQLRIAIKR"
     gene            8751..9146
                     /locus_tag="ACHKJF_004160"
     CDS             8751..9146
                     /locus_tag="ACHKJF_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0951422.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMAEIPALAGMITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9154..9900
                     /locus_tag="ACHKJF_004161"
     CDS             9154..9900
                     /locus_tag="ACHKJF_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951423.1"
                     /translation="MATPNPLEPVKGSGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTGTVEKGKTTTLTVTVEPENATDKTFRAISTDPSKATISVKDMTITVTGV
                     KDGKVSIPVISGNGQFAAVAEITVNNVPGG"
     gene            9919..10350
                     /locus_tag="ACHKJF_004162"
     CDS             9919..10350
                     /locus_tag="ACHKJF_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0951424.1"
                     /translation="MFLKTEQFEYNGVSVMLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            10377..10790
                     /locus_tag="ACHKJF_004163"
     CDS             10377..10790
                     /locus_tag="ACHKJF_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0951425.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            10771..>13088
                     /locus_tag="ACHKJF_004164"
     CDS             10771..>13088
                     /locus_tag="ACHKJF_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0951426.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTASAV
                     EQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGLIPMFRGLAGAVSLPAVGITSLVAATGALAYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTVDRMLALSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGVDDQTRRLKENMGMLETWADKTGKAFKSMWDAILDIGRPDTAQEMLIKAETAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQAELNKALRDGKILQADYNTLMASAKKDYEST
                     QKKTKSSGVRVSAGERQEDRAHAALLALETELRTLEKHSGANEKISRQRRDLWKAESQ
                     YAVLKEAATKRQLSGQEKSLLAHEKETLEYKRQLAELGDKVEYQKRLNELAQQAARFE
                     EQQSAKQAAISAKARGLTDRQAQRESEAQRLRDVYGDNPQALARVTGALKQTWADEDM
                     LRGDWLAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGAEADWRGFTR
                     SVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASTGTAIEAAAANFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYR"
BASE COUNT         3056 a         3084 c         4101 g         2847 t
ORIGIN      
        1 cattatcatt ttctcgggtc ctttccggca tatggacccg ttacggggcg gcgacctcgc
       61 gcgttttcac tatttatgaa aattttctgg ggaaaatcat gtcggtactg attatatgta
      121 atgtattgtt tttaaacggt acgtaagaaa aaaggaccgt cacaggtgcg tggaagaaag
      181 gcattttcag gccgttttgt gtcgtaactg atttttgaat gaggttgttg tatgaatgtt
      241 aacaaaaaga accttgcgga aatgtttaat gttgatccgc gtacaattga acgctggcaa
      301 actcaggggc tccctcagat atctggtgga ggaaaaggta ttgagatcat ttttgattct
      361 gcgaaggcga tcgaatggta tgcgcagcgt gaagccgata ttgaaaacga aaaactccgt
      421 aaagaggtcg aagatttgag agctgccagc gagactgacc ttcagcctgg taccattgat
      481 tacgaacgtt accgactgac gaaggcacag gcagatgcac aggaactgaa aaatgcccgc
      541 gacagtgggc aggtgattga tactggtttt tgtatgtatg cactggcgcg tcttgcgcag
      601 gacatttccg gtattcttga cagtattcca ctgtatatgc agagacaatt ccctgaattt
      661 cccccttccg cccttgagtt tttgaaatcg gagatagcaa aagcatctaa caagtgtgcg
      721 gatactgcag atgggttacc agatatgctt gcagactaca ttagagagtc aaattcataa
      781 gtttgttttt ccattccggg ctgggttaaa ggggatgatt cggccattgc ctgtgacgcc
      841 agttgagtgg gctgacagac attattatct accaccagag tcttcgtatc ttgctggtcg
      901 ctgggaaact ctgccatttc agatggcaat aatgaactgc atggggaatg acaggatccg
      961 cacggttaat ctgattaaat ctgcccgtgt tggttataca aagatgttac tgggcgtggt
     1021 cgggtatttt atcgagcata aaagccgtaa cactttattg ttccaaccga cagattcatc
     1081 tgctgaagat tttatgaaat ctcacgtgga gtcaacgtta cgggatgtcc cttgtctgaa
     1141 aattctttcc ccttggctgg gacgtaagca ccgggataac acgctgacat taaaaagatt
     1201 cacctctcgt gtggggttct ggtgcctggg cggtgcagcc gccaaaaact accgtgaaaa
     1261 atccgtggac gtggtctgct atgacgaact ttcctcgttc gagccggatg ttgaaaaaga
     1321 gggctcgcca accctgctgg gggataagcg tattgagggc tcggtatggc caaaatctat
     1381 tcgcggctcg acgcctaaaa taaaaggcac ctgccagatc gaaaaagcgg ccaacgagtc
     1441 ggcgcatttc atgcgttttt atgtgccctg cccgcactgt ggggaggagc agtatctgaa
     1501 atttggcgat gaatccacgc cttttggcct taaatgggag aaggacagcc ccgaaagcgt
     1561 tttctatctc tgtgaacatc atggctgcgt gatccatcag tctgagcctg accagagcaa
     1621 cgggcggtgg atctgtgaaa acacgggcat gtggacccgt gacggcctga cgtttttcag
     1681 cgctgcgggt aatgaaattc cgccgccgcg ctccatcact ttccatatct ggacggcgta
     1741 cagtccgttc accacctggg tacagatagt ctatgactgg ctggatgcac tgaaagatcc
     1801 caacggcctg aaaacttttg tgaacaccac gctgggcgag acctgggaag aggccgtggg
     1861 cgaaaaactc gatcaccagg tgctgatgga taaggtcgtg cattacacgg cagcggtgcc
     1921 tgcccgggtg gtttatctga cggcgggcat tgactcgcag cgtaaccgtt ttgagatgta
     1981 tgtctgggga tgggctccgg gagaggaagc ctttctggtg gataaaatca tcattatggg
     2041 gcgtcctgat gaggaagaga cgctgttacg tgtggatgcg gcgatcaaca aaaaataccg
     2101 ccatgcagac ggtaccgaaa tgaccatttc ccgtgtctgc tgggacaccg gggggatcga
     2161 tggtgaaatt gtttatcaga gatcaaaaaa acacggtgtt ttccgggtgc tgccggtaaa
     2221 aggcgcatct gtctatggca agccggtgat caccatgcca aaaacccgca atcagcgggg
     2281 cgtgtatctg tgtgaagtgg ggacagacac cgcaaaagaa attctctatg cccgtatgaa
     2341 agccgatccc acgcctgcgg atgaagccac gtcgtatgcc atccgttttc ctgatgatcc
     2401 ggagattttt tcgcagacag aggcgcagca actggtggcg gaagaactgg tggagaagtg
     2461 ggaaaaagga aagatgcgtc tgctgtggga taacaaaaag cggcgtaacg aagcgctgga
     2521 ctgcctggtg tatgcctacg cggcattacg tgtgtccgtg caacgctggc agcttgatct
     2581 ggctgtactg gcaaaatccc gggaagaaga gacgacccgg ccaaccctga aagaactggc
     2641 agcgaagctg tccggaggag tgaatggtta cagtcgctga actgcaggcg ctgcgtcagg
     2701 cgcgccttga tttattaacc ggtaaacggg tggtgtctgt ccagaaagat ggtcgcagaa
     2761 ttgaatatac ggcggcttct ctggatgagc ttaaccgtgc gatcaatgat gcggagtcgg
     2821 tactggggac aacccgccgt cgccgtcgtc cgctgggagt gaggttatga aacgaacgcc
     2881 tgtcctgatt gatgtgaacg gcgttccgct tcgggagagt ctcagctacc acgggggcgg
     2941 tgcaggattt ggcgggcaaa tggcggagtg gttgccaccg gcgcagagtg ccgatgcggc
     3001 cctgctgccc gcgttgcgtc tggggaatgc ccgtgcagat gatctggtac gcaataacgg
     3061 gatagcggct aatgcggtgg ccctgcataa ggatcatatt gtcgggcata tgtttcttat
     3121 cagctaccgt ccgaactggc gctggctggg gatgcgggag actgcggcaa aaagttttgt
     3181 cgatgaggtg gaggcggcct ggtcggaata cgcagaaggg atgtttggcg agatcgacgt
     3241 ggaagggaaa cgcacgttca cggaatttat ccgtgaaggt gtgggcgttc atgcctttaa
     3301 cggcgaaatc tttgtgcagc cggtctggga tacggaaacc acgcagttat tccgcacgcg
     3361 ttttaaagcc gtgagtccga aacgggtgga cacgccagga cacggtatcg ggaatcgttt
     3421 tctgcgggcc ggggtggagg tcgatcgata tggccgtgcc gttgcgtacc atatctgtga
     3481 ggatgatttt cctcgctccg ggagtggacg atgggaacgg atcccgcgtg aacttcccac
     3541 cgggcgtccg gccatgctgc atattttcga gccggtggag gactggcaga cccgtggggc
     3601 caatcagttt tacagcgtca tggaacggct gaagatgctg gattccctgc aggcaacaca
     3661 gcttcagtcg gccattgtta aagccatgta tgcagcgacg attgaaagtg aacttgatac
     3721 cgaaaaggcc tttgaatata tcgcgggggc accgcagggt cagcaggata atccgcttat
     3781 taatattctg gagaagttct ccaactggta tgacacgaat catgtgacgc tgggcggtgt
     3841 caaaattccg caccttttcc ccggggatga tctgaaactg cagaccgcgc aggattcaga
     3901 caatggattt tcggcgcttg aacaggcgct gctgcggtat atcgccgccg gtcttggcgt
     3961 ttcctacgaa cagttgtccc gtgattactc gaaggtcagt tattcaagtg cccgcgcctc
     4021 cgccaatgag tcgtggcgct attttatggg acgacgaaaa tttattgcag cccggctggc
     4081 cacgcagatg ttttcctgct ggctggaaga ggcacttctt cgggggatta tccgtccgcc
     4141 acgggcacgt tttgattttt atcaggcgcg ttcagcctgg tcgcgggcag agtggattgg
     4201 cgcaggaaga atggccattg acgggctcaa ggaggtccag gaatcagtga tgcgcattga
     4261 ggccggactg agcacgtatg agaaagagct ggcgctgatg ggagaggatt atcaggacat
     4321 tttccgccag caggtcaggg aatccgcaga gcgggaaaaa gccggactct cacgtccggt
     4381 gtggatagca caggcgtatc agcagcagat agcggagagc cgcaggccgg aagaggagac
     4441 aacaccacgt gagacgtaat ctttcacaca ttattgccgc agcattcaat gaaccgctgc
     4501 ttctggagcc cgcctatgcg cgggttttct tttgcgcgct cgggcgcgag atgggggcag
     4561 caagtctttc gttaccacaa cagcaggtac agcttgatgc tcccgggatg ctggctgaaa
     4621 cggacgagta catggccgga ggtaaacgac cggcccgtgt ttaccgggtg gtgaacggta
     4681 ttgcggtact gccggtgacc ggcacgctgg tgcaccggct gggtggtatg cggccatttt
     4741 ccggaatgac aggctatgac ggcattgttg cctgtcttca gcaggcaatg gcggacacct
     4801 ctgtccgggg cgtactgctg gatattgaca gtccgggcgg gcaggccgcc ggtgcgtttg
     4861 actgcgctga catgatttac cgcctccggc agcagaagcc ggtctgggca ctgtgtaatg
     4921 acacggcctg ttctgcggcc atgctgctgg cgtcggcctg ctcccgacgg ctggttaccc
     4981 agacatcccg tatcggctcc attggcgtga tgatgggcca tgtcagctat gccggtcatc
     5041 tggcgcaggc cggagtggat atcacgctga tttatgccgg ggcgcataag gtggatggca
     5101 atcagtttga agccttaccg gcagaggtgt gtcaggacat gcagcagcgt attgatgcgg
     5161 cgcaccggat gtttgccgaa aaagtggcga tgtatacggg gctgtctgtg gaagctgtca
     5221 cggggacaga ggctgccgtt tttgaaggtc agtccggtat tgaggccggg ctggcggatg
     5281 agttaatcaa tgcgtcggat gccatcagcg tgatggctgc ggcgctgaac acacatgata
     5341 caggaggcac tatgccgcaa ttaactgcaa cggaagccgc cgcgcaggag aaccagcgag
     5401 tgatggggat cctgacgtgt caggaagcga aaggacgtga acagcttgcc acgatgctgg
     5461 caggacaaca gggcatgagc gttgaacagg cccgggcgat tctggctgcg gcagcaccac
     5521 agcagccggt ggcatccgcg cagagtgaag ccgatcgcat tatggcgtgt gaagaagcta
     5581 aaggtcgtga acaactggcg gcaacgctgg cggcgatgcc ggatatgacg gtggaaaaag
     5641 cccgcccgat cctggctgct tcaccgcagg cggatgccgg accctcactc cgtgatcaga
     5701 ttatggctct ggatgaggca aaaggggctg aggcgcaggc tgaaaaactg gcggcctgcc
     5761 cgggaatgac cgtggagagc gcccgggctg tgctggctgc gggatcaggt aaggcagaac
     5821 cggtttctgc atccacaacc gccctgtttg aacatttcat ggcgaaccat tcaccggcag
     5881 cggtacaggg tggcgtgcca cagacgtcag cagacggtga tgcggacgtg aaaatgctca
     5941 tggccatgcc atgaagccag tgctgaccat caataggagg ttttaacaat atggtgacga
     6001 aaaccatcac tgaacaacgt gcagaagtac gtatttttgc cggtaatgat ccggctcata
     6061 ccgccacagg cagcagcggg atttctcagg caacaccggc actgacgccc ctgatgctgg
     6121 atgaagccag cgggaaactg gtggtctggg acggacagaa agccggtagt gcggttggca
     6181 tactggtact gccgcttgaa ggcacagaga cggtgctgac gtattacaag tcggggacct
     6241 ttgcgacgga ggcagtccgc tggcctgaaa gtgtggatga acacaaaaag gccaacgcct
     6301 ttgtcggcac agccctgagt cacgcggcgc tgccataaca cgttatcagg ccaccgcgtt
     6361 ggcctgactg atttctgaat gaaaggaact gatttatggg attgtttacg acccgccagt
     6421 tactcggtta taccgaacaa aaagtgaaat ttcgtgcgct gtttctggag ctgtttttcc
     6481 gccgtacggt gaatttccac accgaagagg tgatgctgga taaaattacc ggaaaaacgc
     6541 cggtggcagc ctatgtctcc ccggttgttg aaggaaaagt gctgcgtcat cgtggtggtg
     6601 aaacccgcgt gttgcgtccg ggctacgtca agccgaaaca cgaatttaat taccagcagg
     6661 cggtggagcg ccttcccggt gaagatccgg cgcagctgaa cgacccggcc taccgtcgtc
     6721 tgcgtatcat caccgataac ctcaaacagg aagagcacgc cattgtccag gtggaagaaa
     6781 tgcaggcggt gaatgccgtt ctgtatggca aatacaccat ggaaggggag cagtttgata
     6841 ctgtcgaggt tgatttcggg cgctctgaag gaaataacat tgagcaggct gacggtaaaa
     6901 aatggtctga gcaggaccgt gatacgtttg atccgacgca tgatattgac ctctactgcg
     6961 atcaggccag cggtcttgtg aatatcgcca ttatggacgg tacggtctgg cgtctgctga
     7021 atggctttaa gctgttccgc gaaaaactgg atacccgtcg cggctcaaat tcacaactcg
     7081 aaacggcagt gaaagacctg ggggcggtgg tgtctttcaa ggggtattac ggtgatctgg
     7141 ccattgtggt ggcgaaaaca tcttatgtgg cagaggacgg taccgaaaaa cgttatctgc
     7201 cggagggcac gctggtcctg ggaaatacgg ctgctgatgg catccgttgc tatggtgcca
     7261 ttcaggatgc gcaggcgttg tccgaaggtg tggtggcctc ttcccgttat ccgaaacact
     7321 ggctgaccgt tggcgatccg gcccgtgaat tcaccatgac gcagtccgct ccgctgatgg
     7381 tgctgccgga tccggatgag tttgtggtgg ttcaggtgaa ataatccgtg agcgggggcg
     7441 atatgccccc gtgtcttttt tcacagaggg ctgagatggc aacaaaagaa gaaaatcaga
     7501 aacgtcttcg tgaactggct ggcctgctgg ggcgcgaggc agatatgtcg gggagtgcag
     7561 cggatattgc tcaacgtgtg tctgagtggg aagaggagct tgctgtttcc ccggaaggca
     7621 ttatgcactc tgatgagagc gggtctgatc aaaattacac agacgatggt gagcagttga
     7681 acaacactga tgctccggat gatgttaaag ccgtccgggt acggaagtgc ctgcaagtga
     7741 tggggtattg cccggagaca ggtcgtcccg ttgagctggc gttacggggt atgcgtgttc
     7801 tggtgccatc atcactggca acggccatga tacagcacgg aacggctgag tatgcgtgat
     7861 tttcagaatg cctttgatgc cgcccttgcg ggggtggaca gcacgattgt tgaagtgatg
     7921 gggatccgtg cgcagttcac ctccggagca caacgtggcg gtgaagttca gggggttttt
     7981 gacgatccgg agtcgctggg ttttgccggt ggcggggtcc gtattgaagg aagcagcccg
     8041 tcattatttg tgcggacgga tacggtgcgt gctgtgcggc gtggtgacac gctgaccatt
     8101 aacggcgaga tattctgggt ggatcgtgtt tctccggatg acgggggcag ctgttatctc
     8161 tggctcaacc gtgggcaacc acccgcagtt aaccggcgac gataaacgca gggtgaaatt
     8221 atggcgataa aagggcttga tcaggcgatt gaaaatctga gccgggttcg taaaaacgcc
     8281 attcctgctg cttcagcaat ggccattaac cgcgtggcca caacggcaat aaatcagtct
     8341 gcgtcacagg ttgcccgtga gacaaaggta cgccggaaac tggtaaagga acgctccaga
     8401 ctgaaacggg ccacggtcag aaatccgaat gcaaaaatta tcgttaaccg cggtgatctt
     8461 ccagtgatta agctggggat caggatgctg ggccgtcgtc cgaacagcat acttaaagcc
     8521 ggtcagcatc ggtatcagcg ggcattcatt cagcgattaa aaaacggtcg ctggcatgtc
     8581 atgcagcgtg tggccgggaa aaaccgttac cctattgatg tggtgaaaat cccgatggcg
     8641 gccccactga aacaggcgtt tgatgagaat gttgaccgta tccggcgtga acgcctgccc
     8701 ggagaactgg catccgcgct gaaacaacaa ctgaggattg cgataaaacg atgaaacata
     8761 ctgatatccg tgctgcagtg ctggatgcac tcgagcagca tgaacacggg gcgacgctgt
     8821 ttgatggtcg ccccgttgtt tttgacgaag aggattttcc tgcgatcgcg gtttatctga
     8881 cggatgcaga gtataccggt gaagagttgg atgcagatac ctggcgggcc acactgcata
     8941 ttgaggtgtt tttaccggca caggtacctg attcggagct cgatcagtgg atggaaagcc
     9001 ggatttaccc ggcgatggcg gagatccctg cactggcagg catgattacc acgatggtta
     9061 cgcagggcta tgagtatcgt cgtgatgacg atatggcgtt atggagctct gcggatctga
     9121 cttattccat tacatacgag atgtgaggac gatatggcaa caccaaatcc tcttgagccg
     9181 gtgaaaggtt ccggtaccac actgtgggtt tacaacggca aggctgatgc ttatgcaaac
     9241 ccgttgtcag acgatgactg gcagcgactg gcgaaggtga aggatctgac ccccggcgag
     9301 atgacggcgg aatcctacga tgataactat ctggatgatg aggatgctga ctgggtatcc
     9361 accgggcagg ggcagaaatc tgccggtgat accagtttta cgctggcctg gaagccggga
     9421 gaggaaggcc agaaagggct tataggctgg tttgaaagcg gcgatgtccg ggcctataaa
     9481 atccgttttc cgaatggcac ggtggatgtg tttcgtggct gggtcagcag tatcggtaag
     9541 gccgtgacgg cgaaagaagt gatcacccgc acggtgaaag tcactaacgt gggtaaacct
     9601 tctgtagcgg aagaacgcag caaaattacg ccggtcactg cgattaaggt aacgccgaca
     9661 ggtacggttg aaaaagggaa aacaaccacc ctgaccgtta ctgtggaacc ggaaaatgca
     9721 acggataaga cattcagggc gatttccacc gatccatcaa aagccaccat tagcgtgaaa
     9781 gatatgacga ttactgtgac gggggttaag gatggaaaag tcagcatccc tgtgatttcc
     9841 ggtaatggtc agtttgctgc ggtggctgaa attaccgtta ataatgtgcc gggtggctaa
     9901 agagctgaga gataagcgat gttcctgaaa acagaacaat ttgaatataa cggtgtatcc
     9961 gtcatgcttt ctgagctgtc tgcgctgcag cgtattgagc atcttgccct cctgaaacgg
    10021 cgggcagaag aggctgaagc cagcggcaac ctgcaggtga gtgtggaaga tcttgtcaga
    10081 accggcgcgt ttctggtggc gatgtccctg tggcataacc atccacagaa aacgcagtca
    10141 ccgtcaatga atgaggccgt gatgaagata gagcaggaag tgctcaccac ctggcctgcc
    10201 gatgccattg cccgggcgga agacgtggtg ttgtgcctgt ccgggatgat cgaagctgtt
    10261 cgtccggata ctgatattac tgaagtggcg aaaaataaca cgctgactga tgatgatttt
    10321 tctgcgggaa agtcttcgac ggcgagctga actttgccct cagactggcg cgtgagatgg
    10381 ggagacccga ctggcgcgcc atgcttgccg ggatgacatc caccgaatat gccgactggc
    10441 accgttttta ccgcacgcat tattttcagg atacccagct ggatatgcat ttttccgggc
    10501 tgacgtacgc tgtactcagc ctgttttttt gcgatccgga tatgcatccc tctgatttca
    10561 gtctgcttgt cccccggcat gaggaagagc aggtggagag gccggatgag gacaaaatgc
    10621 tgatgcagaa agcggcagga cttgccggag gcgtccggtt cggtggggac ggagggcgcg
    10681 atattttatc gtctgcggat gtggcggatg tcatggtgga tgatgccgca ttaatgatgg
    10741 cttcagcggg gattccggga ggtgtgagat atgtcccagc cggttggtga tcttgttatt
    10801 gaccttagtc tggatgctgt ccgtttcgat gagcagatga gccgggtaag gcgtcatttt
    10861 tcaggactgg agactgacgc cagaaaaacc gccagtgctg ttgagcaggg gctgagccgc
    10921 caggcgctgg ctgcacaaaa agccgggatg tccgtcgggc agtataaagc cgccatgcgt
    10981 atgctaccca tgcagttcac cgacgttgcc acgcagcttg ccggtggtca gaatccctgg
    11041 ctcatcctgc tgcaacaggg cggtcaggtg aaagactcgt tcggtggtct tatcccgatg
    11101 ttccggggac tggcgggagc cgtctcgcta cctgccgtcg ggatcacatc tctggtcgcc
    11161 gcgacaggtg cgctggcgta tgcctggtac cagggggatt ccacgctttc agcgtttaat
    11221 aaaaccctgg ttctttccgg taatcagtcc ggactgactg tcgatcgcat gctggcgctc
    11281 tccagagccg gacaggcagc agggctgacg tttaaccagg cgggagagtc actggcagcc
    11341 ctggtcagtg ccggtgtgcg tggtggtgag cagtttgatg ccattaacca gagtgtggcg
    11401 cgttttgcgt ctgcctccgg tgtggaagtg gacaaggttg cagaggcttt cggaaagctg
    11461 accaccgacc cgacgtcggg actgatggcg atggcgcgcc agttccgtaa cgtgacggca
    11521 gagcagattg cgtatgttgc acaactgcag cgttccggtg atgaggccgg ggccttacag
    11581 gcggcgaacg aggccgcaac gaaaggggtt gatgaccaga cccgccgcct gaaagagaac
    11641 atggggatgc tggaaacctg ggcagataaa acagggaagg cgttcaaatc gatgtgggat
    11701 gccattctgg atattggtcg tcctgatacc gcgcaggaga tgctgattaa ggcagagact
    11761 gcgtttaaga aagcagacga catctggaat ctccgcaagg atgattattt tgttaacgat
    11821 gaagcgcggg cgcgttactg ggatgatcgt gaaaaggccc gccttgcgct tgaagccgcc
    11881 cgaaagaagg ctgaacagca gagtcaacag gacaaaaatg cgcagcagca gagcgatacc
    11941 gaagcgtcac ggctgaaata taccgaagag gcgcagaagg cttacgaacg cctgcagaca
    12001 ccgctggaga aatataccgc ccgtcaggca gaactgaaca aggctctgag agacgggaaa
    12061 atcctgcagg cggattacaa cacgctgatg gcgtcggcga aaaaggatta tgagtcaacg
    12121 cagaaaaaaa cgaagtcgtc aggtgtcaga gtgtcagccg gggagcgtca ggaagaccgg
    12181 gcgcatgcag ccctgctggc gcttgaaacc gagctccgga cgctggagaa gcacagcggt
    12241 gcgaatgaga aaatcagccg gcagcgccgt gatttatgga aagcggaaag tcagtatgcg
    12301 gtcctgaaag aggccgccac gaaacggcag ttatccgggc aggaaaaatc cctgctggcc
    12361 catgagaaag agacgctgga gtacaaacgc cagctggctg agctgggcga caaggttgaa
    12421 taccagaaac gcctgaatga gctggcacag caggcggcac ggtttgaaga gcagcagagc
    12481 gcgaagcagg ccgccatcag cgcaaaagcc cgtggtctca ccgaccgtca ggcgcagcgg
    12541 gagtctgaag cgcagcgtct tcgtgacgtg tatggcgata atccgcaggc gctggcccgg
    12601 gtcaccgggg cactgaaaca gacatgggcg gatgaagaca tgctgcgcgg tgactggctg
    12661 gccgggctga agtccggctg gggcgagtgg gcggaaagtg cgacggacag tttttcgcag
    12721 gttaaaagtg tggccacgca gacctttgat ggtattgcac agaatatggc ggcgatgctg
    12781 accggtgcag aggcagactg gcggggattc acccgttcgg tgctgtccat gatgacagaa
    12841 atcctgctta aacaggccat ggtgggcatt gtcgggcgta tcggcagcgc cattggtggt
    12901 gctttcggtg gtggcgcatc cgcttccacg ggaacggcca ttgaggctgc ggcggcgaac
    12961 tttcattttg cgaccggcgg atttacgggg acgggcggca aatatgagcc tgcggggata
    13021 gttcaccgcg gggagtttgt tttcacgaag gaggcaacca gccggatagg tgtggggaat
    13081 ctttaccg
//
LOCUS       DAZGXZ010000133        12999 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000133 DAZGXZ010000000
VERSION     DAZGXZ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.133"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..278
                     /locus_tag="ACHKJF_004165"
     CDS             <1..278
                     /locus_tag="ACHKJF_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951427.1"
                     /translation="YNHINNENKKIKIPVLISPVVMMMISLITLTDNIIKQQRILYLC
                     GEISKKDFYSNRRVVLKKFSNVRLKLHHNRKEYSEKLSTLIEKKGMQ"
     gene            complement(846..1196)
                     /locus_tag="ACHKJF_004166"
     CDS             complement(846..1196)
                     /locus_tag="ACHKJF_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951428.1"
                     /translation="MKDNQNQSLQVGDATIRIGDIFHTHWGYEQTNIEFYQVVSLHGK
                     KTVGLREIAREVVEHTSWCSAEIRPVPDKFIDEAIHKRRVIAKWGSVLVRFSDVTNAW
                     RTSPEALHHCSWGY"
     gene            complement(1383..1886)
                     /locus_tag="ACHKJF_004167"
     CDS             complement(1383..1886)
                     /locus_tag="ACHKJF_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951429.1"
                     /translation="MHQEQDIADALHESLAYFNRYCRYSLKRLAADYQEEIDNYQDDV
                     WEAPQRAARLSAAVKNYKTSRMIEFIFYMAIKLNLDLTPLVVKRLCSCLFQRTGSQEL
                     IVEIFGKKQRVNRSADNTMERINDVTERYRKSATRHWRKTLSDIEKVKKGYQKEIRAR
                     RKKKSGK"
     gene            complement(2266..2319)
                     /locus_tag="ACHKJF_004168"
                     /pseudo
     CDS             complement(2266..2319)
                     /locus_tag="ACHKJF_004168"
                     /inference="COORDINATES: protein motif:HMM:NF023783.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2300..2557
                     /locus_tag="ACHKJF_004169"
     CDS             2300..2557
                     /locus_tag="ACHKJF_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0951430.1"
                     /translation="MQARVAERVEKASIQLALSALRDELNISQTELATMMGVKQPSVA
                     RMEQTDNDPRISTLKRYVKALGGELSLDVTLPTGKRVAFHL"
     gene            2576..2866
                     /locus_tag="ACHKJF_004170"
     CDS             2576..2866
                     /locus_tag="ACHKJF_004170"
                     /inference="COORDINATES: protein motif:HMM:NF036629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5397 family protein"
                     /protein_id="HFP0951431.1"
                     /translation="MRTLEEVIAAQSPESQAIIKAMADKMRLEIGVKTMNTVQIPTGT
                     IKTFGLLGIPYEVGEPAQELPDGDILVNIILVQTGEKELYKLSKLLQDPDAE"
     gene            2870..3148
                     /locus_tag="ACHKJF_004171"
     CDS             2870..3148
                     /locus_tag="ACHKJF_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HFP0951432.1"
                     /translation="MYAISFDLTVADTEKHHPKGVSQAYNDISSTLKRYGFERIQGSL
                     YTNSDENMANLFQAMNALKALEWFPNSVRDIRAFRIEQWSDFTSSIKG"
     gene            complement(3294..3785)
                     /gene="yjeJ"
                     /locus_tag="ACHKJF_004172"
     CDS             complement(3294..3785)
                     /gene="yjeJ"
                     /locus_tag="ACHKJF_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP0951433.1"
                     /translation="MEYTPIALSPGAMLHKGKFIGYVMKIRFSECSGDHLFYLRYDMV
                     VELARALARKTHQMYLKGTFSSDESSQATLEMEKDYKQHDIYLTSDEISSPSVNNFVA
                     LLDYNVRDASLLLSLIKNDNSKIDISIPEAAVEIFTGYMMNTVIKAGGEALSEKLISD
                     IGV"
     gene            complement(3814..4023)
                     /locus_tag="ACHKJF_004173"
     CDS             complement(3814..4023)
                     /locus_tag="ACHKJF_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partition protein ParG"
                     /protein_id="HFP0951434.1"
                     /translation="MSKMKLGNHKATNTALDALKKPAGATRKLQMNIPEELHLAFKLA
                     CVEDGVDMTQVNIELIQNWLKSRNL"
     gene            complement(4020..4643)
                     /locus_tag="ACHKJF_004174"
     CDS             complement(4020..4643)
                     /locus_tag="ACHKJF_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0951435.1"
                     /translation="MPKVIAFLNGKGGVSKTTSAVNIATALARKGYKVVVVDTDPQGS
                     VSNWYDDSKCVFDVAEATTEKEIYRLRKALPLHDFIIIDGAAAISAISAAAVMVSDLV
                     IIPVTPSPLDFAACGAILAVIEARQDLRPVIARFLITKKVQQTHMLSLLKESIAETSI
                     PALKTGTSYRQSYIKSMLDGGTIFDTKDSAAKGEISVITEEILGLLK"
     gene            complement(4963..5313)
                     /locus_tag="ACHKJF_004175"
     CDS             complement(4963..5313)
                     /locus_tag="ACHKJF_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951436.1"
                     /translation="MKIKLGSGSVSVNGVVYEGKGKVISILNNEVIIDGVVHGKAVAL
                     PEITVIVNGHVDSLHTELGNIIINGNAGMVKTASGDVSCENVSGNVESMSGNVNCKNV
                     YGGVKTLSGNIYKE"
     gene            complement(5346..5621)
                     /locus_tag="ACHKJF_004176"
     CDS             complement(5346..5621)
                     /locus_tag="ACHKJF_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951437.1"
                     /translation="MSQYRLTGKSASRSYELLVGWDTPLSRFFLVIEPELDEPVYSNI
                     YEKDPSSLTLEFFQSVLERYGIENVSLLPGHESGLYEKLHDDRRNNN"
     gene            complement(6383..6868)
                     /locus_tag="ACHKJF_004177"
     CDS             complement(6383..6868)
                     /locus_tag="ACHKJF_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951438.1"
                     /translation="MNNLRDKKSNPVITLIAVVISLFFLPAIAAGLGTMGEIYIDGLR
                     ELPSFGIKAISVAIWMLLVAYLMCCFMKAKKDFIRLPAILTFIGTVLLPFVSSFQKMM
                     IISGSDVSTKATFLSKVTEGLGTMKSGFMFIVPALLCAIASIMLAVGMINILRNRYMG
                     R"
     gene            complement(7079..7729)
                     /locus_tag="ACHKJF_004178"
     CDS             complement(7079..7729)
                     /locus_tag="ACHKJF_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001478769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951439.1"
                     /translation="MMVNTKKFVMIALSSLLLAGCDSAKDANKSNFEKAINTKLEKEC
                     ITISPLGRFDSKPYPVQLSVAQTNRYTSQERADEINTKEFSMFDALVKAGLLTVKDTL
                     VDETIGFSKTGKKVPGREYALTENGKKYLKSPERPDFCIGHYKVDEIIDFTEPGDAMG
                     MKITRVNYTFSPTNIAEWAKSDDIQAAFPLLKFNLKEKQKNRLSLVLKNDGWSAER"
     gene            complement(7795..8103)
                     /locus_tag="ACHKJF_004179"
     CDS             complement(7795..8103)
                     /locus_tag="ACHKJF_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcgAII protein"
                     /protein_id="HFP0951440.1"
                     /translation="METFDTDKFRSELDLLSKRIMPGCGLVFELYQRRLSAAIDEFIA
                     RLPKEQHAQAFELARQEFDYLSAEEIADEIRRDAEKGYCCHGFDRDCCPLGCGDLDDY
                     "
     gene            complement(8794..9429)
                     /locus_tag="ACHKJF_004180"
     CDS             complement(8794..9429)
                     /locus_tag="ACHKJF_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA family replication protein"
                     /protein_id="HFP0951441.1"
                     /translation="MSRKRRRFLNRWIKSLATVAGRVKIKNELRRRIRHNRYWVNEAN
                     KFGIEALCELMLAILDDLDLRSWETRHNLETLAERAGLVTRSDAGRKSISRASRGCDR
                     LVWLNAIITDKAPFNPYDARCACKHIEVTEDFFAILGIPLKLVYRERARLLKADPNEI
                     IYSGDARLIAIRVQNWTRKAAAGLARMKAKRDAARQRKKEYYAHPPTPIPL"
     gene            complement(10011..10277)
                     /locus_tag="ACHKJF_004181"
     CDS             complement(10011..10277)
                     /locus_tag="ACHKJF_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFP0951442.1"
                     /translation="MMMVCILVVSNNGREELIDFNPDHDLAHIIKSYRTPENRMVCII
                     QDGKRILRWDRSYASRAKNHWRKVDPDSFEILGSVEYLRYVGQG"
     gene            complement(10515..11483)
                     /locus_tag="ACHKJF_004182"
     CDS             complement(10515..11483)
                     /locus_tag="ACHKJF_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0951443.1"
                     /translation="MISPAIDYFAANLPYRPYHANELLYGLRIDKAAKAVLARYIQHN
                     PPHAMYWLVFDVDRPIAAIDWNDLNAPTPNFTVKNPVNGHAHLFYGLKTAVRVAKDGS
                     IKAIEFAAAVERGLRDKLKSDTGYAGLICKNPLHTYWQVVEWRGELYELAELADYVDL
                     YAPTAANDEQAFDIDFSYGLGRNCWLFNLTRIWSYTAIRQGWPDFPQWKEAVIQRVEM
                     YNVQLSVPLSHKECVCIGSSIAKWTHKRFTEAGFEQYVADTHTSEIQARRGRKNRSEV
                     QAVKGRKGGKISKRGVVEDSARSLKPWVELGISRATYYRRRANETK"
     gene            complement(11711..12154)
                     /gene="ssb"
                     /locus_tag="ACHKJF_004183"
     CDS             complement(11711..12154)
                     /gene="ssb"
                     /locus_tag="ACHKJF_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0951444.1"
                     /translation="MARKGVNEVTLVGFVGNAPVISQASSGDKIAVISVATTETWTDR
                     KTGAEKEKTEWHRVVIFGKTAEITEQYVKKGTLLYIRGKLQTRKWLDRDNIERYSTEI
                     IVNPAGGEMQMLGSRQDKVTGGKAGNEIPENGAGSTDKQQSNETD"
     gene            complement(12170..12529)
                     /locus_tag="ACHKJF_004184"
     CDS             complement(12170..12529)
                     /locus_tag="ACHKJF_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbM/KikA/MpfK family conjugal transfer protein"
                     /protein_id="HFP0951445.1"
                     /translation="MTKIKILILAAFVSFGTVHSASAENGSDALSLTGEFLEQPVNSS
                     DPCTVFLCMAGMLKGESLGECSGAKKKFFSIIKRKHGSFHPGRTFNARKAFLGDCPSA
                     PGDIVGKILDKYGKLRW"
     gene            complement(12532..>12999)
                     /locus_tag="ACHKJF_004185"
     CDS             complement(12532..>12999)
                     /locus_tag="ACHKJF_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0951446.1"
                     /translation="REAVIAEINAVSEPVVEKTGPENKESTTGIKISEKIQAIINKYA
                     EKYQISETLLKALIKTESGFNPNAVSRKGAKGLMQLMPVHTKRHNADPFDVETNVKIG
                     TGHLSYLLDKYGDLRLALAAYNAGEGAVDKYGGIPPYKETQQYVNKIMAMLGG"
BASE COUNT         3856 a         3022 c         2349 g         3772 t
ORIGIN      
        1 tatacaacca tataaataat gaaaacaaaa aaataaaaat acctgttctt atatcgcctg
       61 ttgttatgat gatgatatcc cttatcaccc ttacagataa catcattaag caacaacgga
      121 ttctttatct ttgtggagaa atatcaaaaa aggacttcta tagcaataga agagttgttc
      181 ttaaaaagtt ttctaacgtt aggctaaaat tacatcataa tcgaaaagaa tacagcgaaa
      241 aactttcaac actgatagaa aaaaagggaa tgcaatagca ttcccttaca gttcagaatc
      301 agatcaataa cggtctagat tctgacgaat ctgacaaatg aaatattcgg cttcaaactc
      361 cgtaatctca tttgtcacga gcgagtatac aatcaaatga acaatacctg ctcgtagatc
      421 aattacagca tgaataactg ccatactcct gaaaagatat gttctcaata atcgagaata
      481 cctatcattt tcgcgaagat tgcattcatt cagcatacca tcagcttgag cctcaacaat
      541 gaaatcagtg aatgtatcaa cagaaacaat cgactgatag catcgttctc ccattacaga
      601 atcaaatgga gatctgcaag ggatcaggtt taacgatgat aaattgttta taacactagt
      661 cataaccaga ctcctgtatt cagaagctgc gttacgctgc cgaagcaacg taacgttgct
      721 tcgacagttg tgtgaatgtt ctttgatgct gaaacgtcag aacatcaaaa ttgatgctgc
      781 atcatctgac agaaccaatc tatcacgcat aaagaagaag gcgcaactgc gccttctcac
      841 aacgtttagt atccccagga acaatggtgt aaagcttctg gtgaagttcg ccatgcattg
      901 gttacatcac tgaaccgtac aaggacagac ccccattttg cgataacccg gcgtttatgg
      961 atggcttcat caataaactt atcaggaaca gggcgaatct ctgcactaca ccaactggtg
     1021 tgctctacga cttccctagc aatctcccga agccctactg ttttctttcc atgtagtgaa
     1081 acaacttgat aaaactcgat attagtttgc tcatagcccc aatgggtatg aaaaatgtca
     1141 ccaatacgaa tagttgcgtc acctacctgg agtgattggt tttgattgtc tttcataacg
     1201 tttacctcaa acatgaggat cacgttacgc tgccagaggc aacgtaacgt tgcctcgaca
     1261 gagaattggt taatgatagg ttacagtctg cccttcccta tcccgcagac agaaaaaaga
     1321 tacactaatg attaaaagat atccactctc cccccacaac ctttgtagtg agtgagttca
     1381 cttcatttac cgcttttctt ttttctccgt gcacgtattt ctttctgata gcctttttta
     1441 actttttcta tatctgataa tgtcttacgc caatgtcttg tggccgattt acgataacgt
     1501 tcagtcacgt cattgattcg ttccattgta ttatcagcac tacgatttac acgctgcttt
     1561 tttccaaaaa tctcaacaat aagctcctgg ctaccagtac gctgaaaaag acaagaacat
     1621 aaacgcttaa caaccagcgg ggttaaatca agattgagct ttatagccat ataaaaaata
     1681 aactcaatca tccgtgaggt tttatagttt tttaccgctg cacttaaccg cgcagcgcgc
     1741 tgcggagcct cccatacatc atcctggtag ttatcaatct cttcctgata atccgcagca
     1801 agccttttta gcgaatagcg acaatatcga ttgaaatagg ctagggattc atgaagtgca
     1861 tctgctatat cctgctcttg gtgcatacta cgccctatgc taacgctact atatgtacac
     1921 attatgcatt agaacttgta caaaacaatc aatccacagt ctcggctacc tcccttcacc
     1981 ggataacttg caaatataag gctttcacat ccaaattcag ccaaaacaag aaacgagatt
     2041 ttattaacaa ataagcaaca taaaaaacga cgaaaaacgc ctaggaaaac atgatctttt
     2101 gagcggattt ttcagatctg acaacgaact gtgatccggt ggtgctgata acgtccatta
     2161 tgttaaatag ccctgttttt ctggaaatta tagtgtattc gttataatgc agtatataat
     2221 gaataccgtg aacatcttgc aaaactggag aaataacatg gctacctaca gagaactact
     2281 ggctaaagag agtcctgaaa tgcaggcgcg cgtagccgag cgagttgaaa aagccagcat
     2341 tcagctcgca cttagtgctt tacgtgatga gctgaacata tcccaaaccg aactggctac
     2401 aatgatggga gtaaaacaac catcagttgc caggatggag caaactgaca acgatccgag
     2461 aatatcgacg cttaaacgtt atgtaaaagc tttgggcggc gagttaagcc tggacgtgac
     2521 attgcctaca ggcaagcgtg tagctttcca cctgtaaaaa attgggcatc aacagatgag
     2581 aacattagaa gaagtaattg cggcccagtc acctgaaagt caggctatta tcaaagctat
     2641 ggctgataag atgaggctgg aaatcggagt gaaaacaatg aatacagtgc agattccaac
     2701 aggtacgatt aagacattcg ggctgcttgg tattccttac gaggtaggag agccagccca
     2761 ggaactaccc gatggtgata ttcttgtcaa cattatactg gttcagacag gtgagaaaga
     2821 gttgtacaaa ctttcaaaac tattacaaga tcctgatgca gagtaagtta tgtacgcaat
     2881 atcatttgat ttaaccgttg cagatacaga aaaacatcat ccgaaaggcg tgtcacaggc
     2941 ttacaacgat ataagttcga cccttaaaag gtacggattt gagcgaatcc agggaagcct
     3001 gtacacaaat agcgatgaaa atatggcaaa cctttttcag gctatgaacg cattaaaagc
     3061 tcttgaatgg ttcccaaatt cagtcaggga tatcagagca ttcaggattg aacagtggtc
     3121 tgacttcact tcgtctataa agggttgata tcctccacgc cctaatgtcg tggaggacgg
     3181 aaggttaagc ctagatcttg ccccctaccg gaaagcgagt ggcctttaag atttagttca
     3241 aaaggggcga aaagcccctt ttttgagcgt tcgcgtttac ctggcagcaa taattaaact
     3301 ccaatatctg atataagttt ttcagataaa gcttctcccc ctgccttaat aacagtattc
     3361 atcatataac ctgtaaatat ttccaccgct gcttctggta tactaatatc aattttagaa
     3421 ttatcatttt tgattagtga taataataat gatgcatccc ttacattata atctaacaat
     3481 gcaacaaagt tatttacaga tggtgacgat atttcatcac ttgttaaata aatatcatgc
     3541 tgcttataat ccttttccat ttctaacgta gcctgactac tttcatctga actaaaagtc
     3601 cctttaagat acatctggtg tgtttttctt gctaatgctc ttgcaagttc aacaaccata
     3661 tcatatctca aataaaaaag atgatctcca gaacactcgg aaaatctgat cttcatgaca
     3721 taaccaatga atttcccttt atgcaacata gctcctggac ttaacgctat tggtgtatac
     3781 tccataaatc ctccatatgc atcataacaa aaactataag ttacgactct tcaaccagtt
     3841 ttgaattagt tctatattta cctgtgtcat atcgacacca tcttccacac aagctaactt
     3901 aaatgcaagg tgtaactctt ctggaatatt catttgtaat ttcctggttg ctccagcagg
     3961 tttctttagt gcatccagag cggtgtttgt tgccttatga ttcccaagtt tcattttact
     4021 cattttaata gtccaagtat ttcttctgta atgactgata tttcaccttt agcagcacta
     4081 tcttttgtat caaatatagt accaccatct agcatagact ttatatatga ctgcctgtac
     4141 gacgttcctg tttttaatgc tggtatgctt gtttctgcaa tagattcttt cagcaaagat
     4201 agcatatgcg tttgctgtac ttttttagta atcaaaaagc gagcaatcac cgggcgtaaa
     4261 tcctgccgag cttctatcac tgccaatatc gcgccacatg cagcaaaatc aagcggcgaa
     4321 ggagtaactg ggataatgac aagatcagaa accataacag cagctgccga tatcgccgaa
     4381 atcgccgcag caccatcaat aatgataaaa tcgtgcaaag gaagcgcctt ccgcaaacgg
     4441 taaatttctt tttctgtcgt agcttctgct acatcaaaaa cacacttact gtcatcatac
     4501 cagttactaa cacttccctg aggatcggta tccacaacga caactttgta tcccttacga
     4561 gcaagagcag tagcaatatt gactgccgag gttgtcttag acaccccccc cttaccattt
     4621 aaaaatgcta taactttagg catttgcccc tcccctttgt tcacatagta cctctgccaa
     4681 acattataat aatctcatac tcatagaatt atgcaatcat aaaatcactt gattttatga
     4741 ttatcgaatc aagtaattgt ataccaaaaa atcaggggta cgcagatacg gcagataacg
     4801 aaaaacagtc cctggattga tgattttgtg aactatctca ttaagaaaaa ataaatacct
     4861 gataaaatga aaatggtttc tgcatataac ctggaaatgt agcaacccaa tacctcttct
     4921 gtactacccc cgtacaatat attgtacggg gttttaatga actcattctt tatatatgtt
     4981 cccagataat gttttaactc caccataaac atttttacaa ttaacattac cactcataga
     5041 ctcaacattt cctgaaacat tttcacaaga aacatccccc gatgcagttt taaccatacc
     5101 agcattacca ttaatgatta tatttcccaa ctcggtatgt aatgaatcaa catgaccatt
     5161 aacaataaca gtaatttcag gaagagctac ggctttccca tgaacgacac catcaataat
     5221 gacttcatta tttaatatgg aaataacttt cccctttcct tcatacacaa caccatttac
     5281 agataccgaa cctgacccta gttttatttt catcgcaata ttcctgcata ttccattaaa
     5341 aaccgctagt tattatttct tctatcatca tgcaatttct catacaaacc tgactcatga
     5401 ccaggcagca gagatacatt ctctatcccg taacgttcaa gaactgactg aaaaaactca
     5461 agcgtcaaag atgatggatc tttctcataa atattcgaat ataccggctc gtcaagttct
     5521 ggctcaataa caagaaaaaa cctggaaagc ggggtatccc agccaaccag aagttcataa
     5581 ctacgcgatg ctgattttcc tgttaaacga tactggctca taactccctc ctgaataatt
     5641 cacgctcaaa aacaatcata taatcatatg attacaaaat caagggatta tatccttttg
     5701 ttattcttat agggctgcgt ttgggaaacg aacaatattg tacggttgcg ctatcgcggt
     5761 tgggtgtact cccgtgctat acggcgtttt caggggcttt ggccttaatc ggataaccta
     5821 aatgcgacat agcaagagcg ataacttaaa ggcgatagcc gctgatgtta aatcaatacg
     5881 ttagaatctt aaattgatta aataatgcgc ttaacgtaca aaaatttccg tttcccaaac
     5941 cgccccctta taggggttcc gtgggaattt ctgcgataac acccgctaag cggagatacg
     6001 tctggataac gcaaatgatt gcggtatgca ggccaggcgg tcagattttc agccgtgcca
     6061 gttcctgtag ggctgcgtta gggaaacgta caatattgta cgtttcccta accgacccct
     6121 tactgatcgc ggaaaaatcc aggtttccgg cttacccccc cctgtagcta ttaagtcgtt
     6181 tatctggcaa atataattga agcaagaagt aggctaatca tcgtcgttaa caacaaagtt
     6241 attttatcgt tatatttcaa tataacgata aaattcaaaa tagcgagaga caccaccacc
     6301 cactcataaa aattatctgt tatcagttcc acattaactc ccttctataa tcaaatcaac
     6361 ctcacaatca ccgcacatca gattagcgcc ccatataacg attacgtaaa atatttatca
     6421 ttccaacagc cagcataatg gaagctattg cacataataa agcaggtact ataaacataa
     6481 atccactttt cattgttccc agaccttctg taaccttgct caaaaaggtc gctttggtgg
     6541 aaacatctga accggatata atcatcatct tttgaaaact acttacaaaa ggcaggagaa
     6601 ccgtcccaat aaacgtcagt atagcaggta agcgaataaa atctttctta gctttcatga
     6661 agcaacacat cagatatgca actaaaagca tccagattgc aacacttatc gcctttatac
     6721 caaaagacgg taattcacgc agaccatcaa tgtaaatttc tcccatagtc cccagtcctg
     6781 cggcgattgc aggaaggaag aataaagaaa tcacgacagc aattaatgta ataacaggat
     6841 ttgatttttt atcacgtaaa ttattcataa ataatatctc ccacttgatt acaataatta
     6901 tctatccaaa cactcataca caacaatctt ataatcatat gattataaaa tcaagagatt
     6961 atttaatcct ccttattttt caatcatctg ttcagcataa cgagaagtag cggcagagtg
     7021 agagtgctac gtgaaatcag caaaagaggg agaccacgta gcaacgtcat ttatctacct
     7081 atcgttcagc ggaccatcca tcatttttca aaaccagaga tagacggttt ttctgtttct
     7141 ctttcaaatt aaacttaaga agtggaaatg ctgcctgaat atcatcgctc tttgcccatt
     7201 cagcaatatt cgtaggactg aatgtgtaat tgacccttgt tattttcatt cccatagcat
     7261 caccaggttc agtaaaatca atgatctcat caaccttata atgaccaata cagaaatcag
     7321 ggcgctctgg agatttcaga tatttttttc cattctctgt cagagcatat tcacgcccag
     7381 gcactttttt acctgtttta gaaaatccaa tagtttcatc aaccagggta tcttttactg
     7441 tcaataaacc agctttaacc agtgcatcaa acatggaaaa ctctttggta tttatttcgt
     7501 ctgcccgttc ctgagatgta tatcgattag tttgggcgac agataattga actggataag
     7561 gcttactatc aaacctccca agaggactga ttgtaatgca ttccttctca agtttcgtat
     7621 taatcgcttt ttcaaagttt gatttattgg catccttcgc tgagtcacac cctgccaaca
     7681 ataatgaact taacgcaatc atcacaaatt tttttgtatt aaccatcatt tttcctcttt
     7741 atcagaaaaa atcagcctat attttcagta tgtaaatata tttatccctt taaattaata
     7801 atcatctaaa tcaccgcaac caagagggca gcaatcgcga tcaaagccgt ggcaacaata
     7861 gcctttttct gcgtctcggc gtatttcatc tgcaatctct tctgcagaaa gataatcaaa
     7921 ctcctggcga gccagttcaa acgcctgagc atgttgttct ttcggcaatc tggcgataaa
     7981 ttcatcaatc gcagcgctca aacgacgttg atatagctca aaaacaagac cacaaccagg
     8041 catgatcctt tttgataaaa gatccagttc tgagcgaaat ttatcagtat cgaaagtctc
     8101 cataattatc acctgcgcaa gttatttgat gcgattgttg cctctgattt gggcaagcaa
     8161 ctttcgttga tactgattat aaagtatacg cgcatgtaaa ttcttatgga atatgccgct
     8221 ttagcggcat ggaaggcgcg aaaaatggtt tcatagagtc acagatgcca ggtcgcgccg
     8281 atacccctaa ccagggagtt tcatgtcgcc aggaaccgcg tcagccccct gctaagcctg
     8341 accgcacgga acttacagct attcaggtgg taggtggtcg tagaccgtcg atgttacagc
     8401 aaagataggt gaataatagt tcgattgtga tgatgggata ctgttgtttc taatgccccg
     8461 agcttgcgag gggtgctact ttcgaggcgc ttgcgccgat gtttttaggt tattcacttg
     8521 ttaaagggga taacagtaat gtttttcttt tttgcttctc ttttagtacg taagtaacta
     8581 aaagagaagc aaaagagtaa ttgcggcata tcgtttgcac tttaagtatg attatccaca
     8641 acctgtgcat agtttaccca atgatacagc ttatacctgc tcttttctgt acatggaaaa
     8701 cggaaaataa cccacgctaa gactattttt tgtacagttt tttattatgg acgtgccgat
     8761 acaccgcccc ataaggcggt aagttagcct gtatcagagc ggaatagggg taggtgggtg
     8821 agcgtaatat tcttttttgc gttgtcgagc tgcgtcgcgt ttcgctttca tccgggctaa
     8881 tccggctgcc gctttccgtg tccagttctg gactctgata gctatcagtc tggcatcacc
     8941 ggagtaaata atttcattag gatctgcctt taacagcctg gcacgttccc gataaaccag
     9001 ttttaacgga atgccgagta tggcaaagaa gtcttcggtt acttcaatgt gtttacaggc
     9061 acaccgtgca tcataggggt taaacggcgc tttatctgta atgatcgcat tcaaccagac
     9121 caggcgatca catcccctac tggcacggga aatagattta cgccccgcgt cactgcgtgt
     9181 caccagtccg gcacgttctg ccagtgtttc gaggttatgc ctggtttccc aggagcgtaa
     9241 atcgagatca tccaggatag ctaacatcag ttcgcaaagt gcctctatgc caaacttgtt
     9301 agcctcatta acccaatatc ggttatgccg gatacggcga cggagttcgt ttttgatttt
     9361 gacacgccca gcgaccgtcg ccagcgattt aatccagcga ttgaggaaac ggcgtcgctt
     9421 acgcgacaca acgttatagc atggactttg ttttgccacc cctgacggcg atatgcgggg
     9481 gattaaggaa ttgttctttt cagccaccac cacccgcact tattgccggg cagtagtctt
     9541 gtattgaaaa atgcatttgc tataatcctt tacaggcaaa agcctgcttt ggaccccctg
     9601 attatcttct ttggccccgc aaaagctcta agagagatat cagggggttg ttgcattccg
     9661 ggggctataa attcgctatc cccccacccc tggacgctgt tacgggaacg tcccaccctt
     9721 ataaattcgt caaaaccgca taaaaataac atgcgcagat gttaagatca atctatgcgc
     9781 caaaagccat cacaaagcat gctcagcatt tctctttaat attcataaca ttatataaat
     9841 attaaaactc aaagattcac gtgcaacata acacaaaaag ttaatgctaa tgtacgcatc
     9901 cgttagcatt aacaaaagag caatgaccat catccgtcaa agatagcatt atcataacct
     9961 ggttaaaaaa cagtcttagc atgagttgtt ttccgttttc cacgccccaa ctaaccctga
    10021 ccaacatagc ggagatattc aacagaccct aaaatctcaa aactgtctgg atctacctta
    10081 cgccagtgat tttttgcacg ggaagcataa ctgcgatccc aacgaagaat gcgcttacca
    10141 tcttggataa tacataccat cctgttttct ggtgtccggt atgatttgat aatgtgcgct
    10201 aaatcatgat cgggattaaa atcaatcaac tcttcccgac cgttatttga cacaacaagt
    10261 atacagacca tcatcatact ctccatttgt cttcttgcca tgctcaaaca tcgagcgtaa
    10321 gtgcaaacat caacctctga ctcccttgcg cctcctcgcc cactcgctca cctggcggtt
    10381 cggtcgctgg ggctgcggca ggcgctatca tctcactcat aaaagactca atacctgaaa
    10441 atcggaagat cactaaaggc aaaggatctg tctgcaagga tcaagatccg gtgctgttat
    10501 ctgatatagg ttgtttattt ggtctcattt gccctacgtc gataataggt tgcccgactg
    10561 atccctaact ctacccacgg tttcaaactc cgcgcagaat cctcaacaac ccctctttta
    10621 gatattttcc cacctttacg ccccttaaca gcctgcactt ctgagcgatt cttacgccct
    10681 ctacgagcct gaatttctga tgtatgggta tcagccacat attgctcaaa tccagcttca
    10741 gtgaagcgtt tatgcgtcca tttggcgatg ctggagccaa tgcatacaca ttctttgtga
    10801 gacaaaggca cgcttaactg cacgttatac atttcgacac gctgaatcac cgcttctttc
    10861 cattgcggga aatcaggcca gccctgccgg attgctgtat atgaccagat acgggtcaga
    10921 ttgaagagcc aacaatttcg cccaagccca taactaaagt cgatatcgaa tgcctgttca
    10981 tcgttagccg ctgttggcgc gtacaaatca acataatcag ccagttcagc taactcataa
    11041 agttctccac gccattcaac aacctgccag tatgtatgga gaggattttt acaaatcagc
    11101 cctgcatagc cggtatctga tttaagtttg tcacgtaatc ctcgctctac cgcagcagca
    11161 aactcgatcg ccttaatgct accgtctttg gcaacacgaa ccgccgtttt taacccataa
    11221 aacaggtgtg catgaccatt aaccggattt ttgaccgtaa aattaggtgt gggcgcatta
    11281 agatcattcc agtcaatcgc cgcaattggc ctgtcaacgt caaaaacaag ccagtacatt
    11341 gcatgaggcg gattatgctg tatataacgc gccagcactg ctttagcagc cttatcgata
    11401 cgaagcccat agagcaattc attcgcgtga taaggtcggt atggcaagtt tgccgcaaaa
    11461 taatcaattg ccggactaat catatggccc tgcacttatg cctgaatgac acattatttt
    11521 tcttgccgtt accggatgat ttaaatacaa tttaaatggg cttttatggt cccctgataa
    11581 tgcccacaag ctgactctgc caagtatcaa cctcatgggt attatcatcc ccgtagtaaa
    11641 aaccaaaaac gccggggtca ctcggaactt gcttctgagt gactccgggg ttgaacaagc
    11701 cgaaggcgcg ttagtcggtt tcattgcttt gctgcttgtc cgtactgcca gcgccatttt
    11761 caggaatctc gtttccggct tttcctccgg ttactttatc ctggcgactc cccagcatct
    11821 gcatttctcc ccctgccgga ttaacaataa tttccgtgct gtaacgctct atattgtctc
    11881 ggtccagcca cttacgggtt tgcagtttcc cgcgaatgta cagcaatgtg ccttttttta
    11941 catattgttc cgttatctcg gctgtttttc cgaaaataac aactcgatgc cactcggttt
    12001 tctctttttc agcacctgtc tttctgtccg tccatgtttc agttgttgca acggaaataa
    12061 ccgcgatctt atccccgcta ctggcctgag atatcaccgg agcattacca acaaaaccaa
    12121 ccagggtgac ttcatttaca ccttttcttg ccactgttta ccccttctgt taccagcgta
    12181 attttccata tttatcaagt atcttgccta caatatctcc aggtgctgac ggacaatcac
    12241 caagaaacgc tttccgggca ttaaatgttc ttcctggatg aaaactgcca tgcttccttt
    12301 tgataatgct gaaaaatttt ttctttgccc cactacattc accaagcgat tcccccttaa
    12361 gcattccagc catacagaga aaaacagtac atggatcact actgttaaca ggttgctcaa
    12421 gaaattctcc tgtcagtgac agagcatcac ttccattttc cgcgctggcg ctatgcacgg
    12481 ttccaaatga gacaaaagct gcaagtatca gtatctttat ttttgtcata tttaacctcc
    12541 taacattgcc atgatcttat ttacatactg ttgtgtttcc ttataagggg ggataccgcc
    12601 atatttatca acagcacctt ccccagcgtt atatgcagcc agagcaagcc gtaaatcccc
    12661 gtatttatcg agaagatatg aaagatgccc tgtaccaatt ttcacgttgg tttcaacatc
    12721 aaatggatca gcattatgcc ttttggtatg tacaggcatt aactgcatca gtccctttgc
    12781 accttttctg ctgacagcgt ttggattaaa tccagactcc gttttaatta acgctttcaa
    12841 caaagtttca ctaatttgat atttttctgc gtatttattg attatcgcct gtattttttc
    12901 agatattttt attcctgtgg tcgattcttt gttttctggc cctgttttct ccaccaccgg
    12961 ctctgacacg gcattaattt cagcaatcac cgcttctct
//
LOCUS       DAZGXZ010000134        12952 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000134 DAZGXZ010000000
VERSION     DAZGXZ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.134"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1345
                     /gene="paoC"
                     /locus_tag="ACHKJF_004186"
     CDS             <1..1345
                     /gene="paoC"
                     /locus_tag="ACHKJF_004186"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP0951447.1"
                     /translation="LQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAG
                     ANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQV
                     DPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLE
                     KSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVS
                     AGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEAT
                     AGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNP
                     KTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDD
                     TDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1355..2311
                     /gene="paoD"
                     /locus_tag="ACHKJF_004187"
     CDS             1355..2311
                     /gene="paoD"
                     /locus_tag="ACHKJF_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP0951448.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            2362..2700
                     /locus_tag="ACHKJF_004188"
                     /pseudo
     CDS             2362..2700
                     /locus_tag="ACHKJF_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            2989..4266
                     /locus_tag="ACHKJF_004189"
     CDS             2989..4266
                     /locus_tag="ACHKJF_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0951449.1"
                     /translation="MRYKPNQYIICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRMQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLSQQSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRESYEAFSNK
                     ELLQVLQVFSGNSAENKEMMALCLIGLYTGMRINEIASLTIDDVKEIEGVLCFEITQG
                     KTKAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKR
                     KALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASP
                     VELKNYIELLHYPEIEKGLSINKKSNLRRKTTP"
     gene            complement(4348..5061)
                     /locus_tag="ACHKJF_004190"
     CDS             complement(4348..5061)
                     /locus_tag="ACHKJF_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFP0951450.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNTNNSASTVKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVAIKTQPSKNIGANMKKRVN
                     TEKPSSEGEDTITPAFKQE"
     gene            complement(5114..6928)
                     /locus_tag="ACHKJF_004191"
     CDS             complement(5114..6928)
                     /locus_tag="ACHKJF_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951451.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDNVTKGYKKAQSEVDKIAQQKAKTEKQNQRAQAAAARAQARQQAQIAARSEKAALK
                     LLDVGSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKIN
                     GNRKAQLAPVKGGSGNSATMGALLFGGATAAAGVMAVSKASEFMANSFANAETQGELI
                     QRARLGGVDVNQMYNITEWAYKNGVDSMMGDQGARKYLDQMKDVRERAAKSYSEAELV
                     KDKKTGKSEWKGGDNAINELLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSDSQ
                     IGQRLEDLGDDLMLTSKYWQRSVKDVQESINQQKASGKWLTETQQDSIVKFRELNRQL
                     SQLSDARQVAFVDGFMKSLDPKVTEEFMKNLSNLTPYFTKLGEASGSLFEAIMKIVNW
                     FNRNDDKTDAIQKNLGDAPPLSNEGMKQHLSNLVPDQYKGAGTATTTPDNTHSLFNTI
                     KGWLFDDNASSVSDVKMSVSEAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVVT
                     EVPLTINSDTSRLSDYVDFTARASRDSFLKSLTLTSLS"
     gene            complement(6943..7146)
                     /locus_tag="ACHKJF_004192"
     CDS             complement(6943..7146)
                     /locus_tag="ACHKJF_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000452041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951452.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGGKND"
     gene            complement(7232..8260)
                     /locus_tag="ACHKJF_004193"
     CDS             complement(7232..8260)
                     /locus_tag="ACHKJF_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0951453.1"
                     /translation="MILGNDYVDLAPLFSAHSTRNYLLSTLDFTDSVGVTSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYAEYVAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVTPM
                     KATVNASSTDTTKIFKEFDQIATDIIEKAQSQAAAVERIVVFCKPEAFSAIRFSAGMA
                     NAFQYVSPLEEGNVVYQRRDLLPGVTAFTIPGTNIDVVKLVDPLHLAHMTADAVAVPK
                     FAKGSNVYQNIYGAASSTFELINAAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGLG
                     FSVQIIVK"
     gene            complement(8279..8641)
                     /locus_tag="ACHKJF_004194"
     CDS             complement(8279..8641)
                     /locus_tag="ACHKJF_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951454.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVESKVFASLPADVECG
                     DLITSTGAKYESGDDIYVVMSEFVTAGKNKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHSVFTS"
     gene            complement(8638..8850)
                     /locus_tag="ACHKJF_004195"
     CDS             complement(8638..8850)
                     /locus_tag="ACHKJF_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951455.1"
                     /translation="MLFLNDKEQIIKYRDEMLKINPQITEMVAKYAGCSVEEVERCVE
                     KYFSPSSPSTPSLNELIKLKAKEITK"
     gene            complement(9182..9520)
                     /locus_tag="ACHKJF_004196"
     CDS             complement(9182..9520)
                     /locus_tag="ACHKJF_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951456.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPAALTEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGAAITEE"
     gene            complement(9522..9932)
                     /locus_tag="ACHKJF_004197"
     CDS             complement(9522..9932)
                     /locus_tag="ACHKJF_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951457.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFQILDNADHYGDF"
     gene            complement(10062..10340)
                     /locus_tag="ACHKJF_004198"
     CDS             complement(10062..10340)
                     /locus_tag="ACHKJF_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951458.1"
                     /translation="MIQYLVKNQVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAGCSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIEFIYSTL"
     gene            complement(10352..11005)
                     /locus_tag="ACHKJF_004199"
     CDS             complement(10352..11005)
                     /locus_tag="ACHKJF_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0951459.1"
                     /translation="MINEEQFVKVPMWILKISQVNGHKFTDSAKNLYCYLKGFDRAFP
                     SYAKIGDVFGITPRAAEERVKKLLEMGLIIKDARPGSSNVYQVVDIPQEEARQEAQTQ
                     QEATVKAVPDQLPPTEDNKQPSFDVLDDWDAPLPWETEETPVSSSEKVANDNEEDVLE
                     NFASLICQQKNTRTGGASFIEFATSLAYKHRLKLPNGIEAYFSKHYPNVYDDFDIPF"
     gene            complement(11057..11224)
                     /locus_tag="ACHKJF_004200"
     CDS             complement(11057..11224)
                     /locus_tag="ACHKJF_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951460.1"
                     /translation="MSETKKPIPRTYLHVDPEIFKVLFAEAKKRQIMVSDLMLEIITE
                     AAENIKQKKGK"
     gene            complement(11245..11433)
                     /locus_tag="ACHKJF_004201"
     CDS             complement(11245..11433)
                     /locus_tag="ACHKJF_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951461.1"
                     /translation="MKWFTSQDVVAAWKRGEINRFKVRMYRNTARRCGYPEREKCFDD
                     ALKIIDELRKAEKEAEKE"
BASE COUNT         3545 a         2835 c         2767 g         3805 t
ORIGIN      
        1 ccttcagcac ttgcgtatcg gtgccgacca gagcgggaaa atcaccgcta tctcacatga
       61 aagctggtct ggaaacctgc ccggcggcac gccggaaacg gcggtacagc aaagcgaatt
      121 actctacgcc ggggcgaatc gtcataccgg cctgcggctc gccacgcttg atttgccgga
      181 agggaacgcc atgcgtgcgc ccggcgaagc ccccggtctg atggcgctcg aaatcgcgat
      241 cgacgaactg gcggaaaaag cgggcatcga tcccgtcgag tttcgcatcc tgaatgacac
      301 tcaggttgac cccgccgacc cgacgcgctg cttctctcgc cgtcagctta tcgagtgctt
      361 gcgcaccgga gcggataaat ttggctggaa gcagcgcaac gccacacccg gacaggtgcg
      421 cgacggggag tggctagtcg gccacggtgt tgcggcgggc tttcgcaata atctgctgga
      481 aaaatcgggt gctcgggttc acctcgaaca aaacggcacc gttaccgtag aaacggacat
      541 gaccgacatt ggcaccggca gctacaccat tctggcccag acggcagcgg aaatgcttgg
      601 cgtaccgctg gagcaggttg cggttcacct cggcgattcc agtttcccgg tttctgcggg
      661 ttctggtgga caatggggcg cgaatacctc cacctccggc gtttacgccg cctgtatgaa
      721 gcttcgcgaa atgattgcct cggcagtcgg gtttgatcct gagcagtcgc agtttgccga
      781 cggcaagatt accaacggta cccgaagcgc cacgctacat gaagccaccg caggcggcag
      841 actgacagcg gaagagagca ttgaattcgg aacactgagc aaagagtacc agcagtcgac
      901 ctttgccggg cattttgtgg aggtcggcgt gcatagcgcg acgggagaag ttcgggtccg
      961 gcgtatgctc gctgtgtgtg ctgcaggacg catcctgaat ccgaaaactg cgcgcagcca
     1021 ggtcattggc gcaatgacta tgggcatggg cgcggcactg atggaggagc tggcggtgga
     1081 tgaccgtttg ggctacttcg ttaatcacga tatggcgggg tatgaggtgc cggtccatgc
     1141 ggatatccca aaacaggagg tgattttcct ggatgatacc gaccccatat cctccccgat
     1201 gaaggccaaa ggtgtcggtg agttgggcct gtgcggcgtg agcgcggcta tcgccaacgc
     1261 ggtgtataac gccaccggta ttcgggtacg ggattatccc atcactctgg ataagctgct
     1321 cgataagctg ccggatgtgg tttaaggaga aacaatgtca tacccgcttt ttgataaaga
     1381 cgaacactgg cataagccag agcaggcgtt tctcaccgat gaccaccgga ccattctgcg
     1441 cttcgccgta gaggcgctaa tgtccggtaa aggagcggtg ctggtgacgc tggtggagat
     1501 acacggcggc gcggcgcgcc cgctcggggc gcagatggtg gttcgcgaag atggtcgtta
     1561 ctgcggtttt gtctctggcg gctgcgtgga ggccgctgcc gcttttgaag cgctggagat
     1621 gatgggctca ggccgcgatc gcgaaattcg ctatggcgaa ggttcgccgt ggtttgacat
     1681 cgttctgccc tgcggcggtg ggatcacgct tacgctccat aaactacgct cggcacagcc
     1741 tctgctcgcc gtgctgaacc gtctggaaca gagaaaaccg gcggggctgc gctacgatcc
     1801 gcaagcacaa tcgttggtgt gcctgcccac gcaaacccga acgggctgga atctcaatgg
     1861 ctttgaggtg gggttcaggc catgcgtcag gctgatgatt tacggacgtt ctcttgaggc
     1921 gcaggcaacc gcgagtcttg cagcagccac aggctatgac agccatatct tcgatctttt
     1981 tccggcctca gccagcgctc agatcgatac cgacacggcg gtcattttgc tgtgccatga
     2041 tctcaaccgg gagctgccag cattgcaggc cgcgcgagaa gcaaaaccct tttatctcgg
     2101 cgcattgggc agccatcgaa cccacacgtt acgtctgcaa aagctccacg agctgggatg
     2161 gtccagggag gagacagcgc aaatccgggc ccccgtcggg atattcccca aagcccggga
     2221 tgcgcatact ctggcactct ccgtgctggc agaaatcgcc tctgtacgtc tccatcatga
     2281 ggaggattca tgcctgcccc cgtcgtcctg atccttgcgg ccgggcgtgg agagcgcttt
     2341 ctcgcctccg ggggaaatac ccataagtgt atcggctggc gtcagtcccc ggaggttgcg
     2401 ccttatcgct ggccatttga agaaaacggg agaactttcg accttgcgat tgaaccgcag
     2461 attacgacta atgatctgcg tctgatgttg aggctggctc ttgccggcgg aggaataaca
     2521 attgccactc aggaaacttt caggccatat attgaaagcg gtaagcttgt atcgctgctt
     2581 gatgactttc ttccacaatt tccgggcttc tatctgtatt tcccacagcg tcgcaatatt
     2641 gcaccaaagc tccgcgccct gattgactac gtcaaagaat ggcggcagca attggtttaa
     2701 atagctgcac ctgcactgcc tgatgtcaga acagtatttt gatgaattgc caaggttaca
     2761 atggcacaaa tgcggcacag gaggaaacgt ggtgtattta aatatggggt aactctttga
     2821 ttttaatggt gccgataata ggagtaaaac actcgattaa tctcgtgtta atgccatcac
     2881 ctacgaccat tactaatcga ttgaatctta tagaaaaaat catcattttc tatgcgttac
     2941 aatcaatttt cacattacaa acgctccgac actttagccg gggagtttat gagatacaag
     3001 cctaaccaat acataatctg tgattcttac ggtaactact atttaaggat cacgctgcct
     3061 gtgtatatgc agcccttttt tgaaggaaaa aggacgtttg tcaggagtct gcatacaagt
     3121 aatcttcgtg ttgcacgtag gaagcgtgat cagattgcag atgaatacca ttgtctacgg
     3181 gagagtgtcg cccctgtaaa cagcacaata gagaacacgc tggaactgtt acgcagtaag
     3241 gctaaatacg ccaaaacagc taccagaatg caagatacag cgtcttcgtg tccgtcattg
     3301 cttaaaattc ttgaaatcta cctgacaatt aacagcacga agaagaagcc agccacttta
     3361 gctaaggcaa gaaaagcggt agagatgttt ctctcctacc gtaaaaagcc tgatattgca
     3421 ttgcaagatg taagccgcac cactgttaca ggctggattg aacacatgca aaaaaccctt
     3481 tcacaacaat caattgcaaa ttatatcagc ccaatggccc agctatggga gttagcttca
     3541 tcacgttacc acgatgcgcc agaaagggcg ctctccccct ggcgagggca taggcttgat
     3601 gtggcacaaa gtagagagag ctacgaggca ttttctaaca aagagctatt gcaggtgttg
     3661 caagtatttt ccggtaattc agcagaaaac aaagaaatga tggctttgtg tcttatcggt
     3721 ttatatacag gtatgcggat caatgagata gcaagtctca caatagacga tgtgaaagag
     3781 atcgaaggtg tgctgtgttt tgaaatcaca cagggaaaaa cgaaagctgc ggcacgtgtt
     3841 gtgcctgtac atagccttat cactccgttg gtgttgtcgc tgcgtgaaaa gcctcataat
     3901 ggctttttgt tctatcacgc cagcattaca gagcgtgctg atggtaaacg ttccacgtgg
     3961 cacacgcaga gatttacaag agctaaacga aaggctttag gggaaaaggg aacagaaagg
     4021 aaagtgtttc attctctgag acacggagta gcacagcttc ttgatcgaaa tcaaattcca
     4081 gaagacagga tcgcccttct cctgggccat acacgcggca atacagaaac attccgcaca
     4141 tatagcaaga atgcagcttc tccagtagag cttaaaaact atattgagct tctacactac
     4201 cctgaaatag agaaaggctt atcaatcaat aaaaaatcaa atttaaggcg taaaacaacg
     4261 ccatagacgc aataaagagg cttcaacata gattggtagc ctcttttata attacatcgt
     4321 tacagagcgt tttagaggcg tttacggcta ttcctgttta aaggcaggag taatagtgtc
     4381 ttcaccttca ctacttggtt tttcagtgtt aacacgtttt ttcatattag ccccgatgtt
     4441 cttagatggc tgtgttttga tagctacagt gttcacagtg ccataagaaa ctaatcttat
     4501 ttgctcaaag ctaagatcaa ataccaggga ttcgcttgcc tcttctgtgt attcgatgcc
     4561 tgttaaaaca acgttctcaa aagttttatt ttctgtaaca agaagaaaag gctgtctact
     4621 gtccataatc tgattcaggt agtcgatagc caatgatctt ctgttcccgt tcacccctgc
     4681 actctttaac aagccaggat cgcttttaat cggtgtttca gtgatccttc ctttaagaga
     4741 aaacttattg tttttaattt gaacgtgatc agacacagta gcaccatttt ctacggggta
     4801 gcttgtaaca tctgccgaac gtctcacgtt cacatcgtca acactgtcaa aagccagtgc
     4861 catataattt tcataaacgt cattaccaga agatgccagc ccactggtaa caatagcaaa
     4921 tccgttctct cctttcacag tagaggcaga gttatttgtg ttttttgtag aatttatatc
     4981 ttgttctgct ttgtaaacag cctgaacgtg ttgttctgga gtatttgctg cacgtatggt
     5041 taaaatcccg gcggtcgcca taataataat attccttgta atgttatggc gggaattacc
     5101 cgccgttaga ttgtcatgat aagctggtta atgttaatga tttcaagaag ctatcccgac
     5161 ttgctctcgc tgtaaaatcc acataatcgc ttaatcttga tgtgtcacta ttaatagtaa
     5221 gaggaacctc tgttaccact tcaaatgtag gattcagatt aaacacagga gataaattat
     5281 aagccggagt tgtcattgca agattattta aagcaccctg tttgagattg gttatcggtg
     5341 cttcgctgac tgacattttt acatctgaca cagaagaagc attatcatcg aaaagccatc
     5401 ccttaattgt gttaaagagg gaatgagtat tatcaggtgt agttgttgca gtgccagcgc
     5461 ctttatattg atcaggaacg agattagaaa gatgctgttt catcccttca ttactcaaag
     5521 gcggtgcatc acctaaattt ttctgaatag catctgtctt atcatcatta cgattaaacc
     5581 agtttacaat cttcataatg gcctcaaaga gacttccaga cgcttcccct agcttcgtaa
     5641 aatacggagt gagattagaa aggtttttca taaactcttc cgttactttt ggatcgagag
     5701 acttcataaa gccgtctacg aatgctactt gtcttgcatc agaaagctgt gaaagctgtc
     5761 tgtttagttc tctgaattta acaatagagt cttgctgtgt ttctgttagc cattttccag
     5821 aggctttttg ctgattaata ctttcttgta catctttaac ggaacgttgc caatattttg
     5881 atgtaagcat caaatcatcg cccaggtctt ctaaacgttg tccaatttgg gagtctgaaa
     5941 accctttttt catcattcca ttaacagcct tagaaattaa tccagccgga ttatcagcaa
     6001 attttttaag gtcgcttttg ttaataacac cgatgtttag aagttcatta atagcgttat
     6061 caccgccttt ccattcagat tttccggttt tcttatcttt caccaattca gcttcactgt
     6121 aggattttgc ggcacgttcc ctaacatctt tcatctgatc caggtatttt ctagcccctt
     6181 gatcacccat catagagtca acgccgtttt tatacgccca ttctgtaatg ttatacatct
     6241 ggttaacgtc tacaccgcca agccttgcac gttggattaa ttctccttgt gtttcagcat
     6301 tagcgaagct gttagccata aattcagagg ctttactaac agccatcacg ccagcagcag
     6361 ccgtagcgcc tccaaataac agagcgccca tagtggcgga attccctgat cctcccttaa
     6421 cgggggctaa ttgagctttt cggttgccat taatcttacg ttgttgttgt tgtaggcgtt
     6481 tcatctggct attcatacgt gcaagcgaga tagcgccttg ctcatattgt tttgctatct
     6541 ctctagcctg tgcaatagct ttatattgtt ccgctacaga gagacggtgc attgcactaa
     6601 tgcttgatcc aacgtctaac agttttaagg ctgccttctc actgcgtgct gctatttgtg
     6661 cctgttgcct tgcctgtgct cttgctgcgg cagcttgtgc tctttgattt tgtttttcgg
     6721 ttttagcttt ctgttgagca atcttatcta cttcactttg tgctttctta tagcctttag
     6781 tgacattatc aaatcttaat ttagctggct tgatgttctc tgcgaattcc tttaagcctg
     6841 taatagagtc tttcgctttt ttcaagctgt ctttatctac actaaatgtt actgtgttct
     6901 taattttaga agtgttaatt tctaacattt aaaatcccct tattaatcat ttttaccacc
     6961 ttctttgctt aagaaatggt gcagagcttg atcaatactc tcgtttagtc tgaacatacg
     7021 ggcttttact ccttcatatg gaacatctag gagagaacat agatatttca gatttaacaa
     7081 tcctctagaa tcattagcat ctaaatgtac gtgtacacca ttttttgatt tcagaatcac
     7141 ttccatattt taatcacctc tcaaaattta aaaaaaataa aaaaaaggcg aagcgtgaat
     7201 acacgccacg cctctattac acgttaaaac attatttaac aatgatttga acagagaagc
     7261 ccaacccgtg gtttacaacc atctggctat tctctgtaac aacgttaaca gcatcaccac
     7321 gagaagattc atagctgtag ctgtacacct cagcaggagc cgcattaatc agttcaaaag
     7381 tgctggaagc agccccgtag atgttctgat atacgttaga gcctttagca aacttcggaa
     7441 ccgctacagc gtctgcggtc atatgtgcaa gatgtagcgg atctaccagt ttaacaacat
     7501 caatgttagt tcctggaata gtgaacgctg tcacccctgg tagcaggtca cgacgctgat
     7561 acacaacatt cccctcttcc agtgggctta catactggaa tgcgtttgcc ataccagcag
     7621 agaatcgaat tgcagagaag gcttccggct tacagaaaac aacaatacgc tctacagcag
     7681 ccgcctggct ttgtgctttc tcgataatgt cagtagcaat ctgatcaaat tctttaaaaa
     7741 tcttcgtggt gtcagtgctg gaagcgttca cagtggcttt cataggtgtc acattaaata
     7801 gcttgcccca ctcaattaac acatcatcag ttttaggcgt gtaagttttg ccagagaaca
     7861 aagaagcagc caatacgctc tcttttgtac gctgatacgc tacgtggtgt ttagcaacat
     7921 actcagcata aatatcagtg agtgtttcct ggaaatcagt gccaggttta cgcttgcctt
     7981 gaacatcaga aggacggatc acatcttcac gaagaaaata cgggatttca ataagccatt
     8041 ctttaccttg ttctcgctta gtaacgttgt gttcagaaga gaaacgagag gtttctttgt
     8101 tgaacagaga ttcgttgctt tcgacaagct gtgaaacagc tactttatga gatgtaaccc
     8161 ctacagaatc cgtaaaatca agggtagaga gtagataatt tctagtgcta tgcgcagaga
     8221 acagaggagc taaatcaacg taatcattac ctagaatcat ttattattat tccttttatt
     8281 aagaagtgaa aacagagtgg aagccttcaa gctgaaagcc ttttttaatt aatgctgttt
     8341 ttgctgcttc acttacagca ttcagggcat cagctttaat gcatacaagg ccagcattgc
     8401 tacgtaaaac atctacaggc ttgttttttc cagccgttac aaattcactc ataacaacat
     8461 agatatcatc tcctgattca tatttagcgc ccgtgctggt gatcaagtct ccgcattcaa
     8521 catcagcagg aagagaagca aaaaccttgc tttctacaga ggcaaacgga tcagtacaat
     8581 aacttacaat atctgtgaaa gtctttcgaa ctggaaatag agtgtctaaa tcaataatca
     8641 tttagtaatc tccttagctt ttaatttgat taattcatta agggaaggtg tagacggaga
     8701 agaaggagaa aaatattttt caacacatct ttctacttct tcgacagaac acccagcgta
     8761 tttagctacc atttcggtaa tttgtggatt gatctttagc atttcatcgc gatacttgat
     8821 gatttgctct ttatcgttta agaacaacat ttatataaac cttgttacct tgtttaaagt
     8881 aagaaataca acttttaagt tgcataacgg gcaaaataaa aggcagcatt taaagccgcc
     8941 tttgtaatta ccctatgtta tgcacactcc aagatgtatg cataacaaag gaaaaagagg
     9001 taataatgac acagtattta cttaaggagg agaagcgggg agcggagatt ttactcccct
     9061 ttaacgacaa gaagaatttt taatatcgaa ccaataaagg aaaaataatc accccatata
     9121 attatattaa catacaatat atgaaatgtc aagatatttt acaacttttt tgttgctttt
     9181 tttattcttc tgtgatagca gcgcctaagc gttgtgcgag ttcgaccaag ccatccccta
     9241 catagcgaac atcagattca atatcttcta tccagtttga tagctcccat atctgatcgg
     9301 ctgtcaggta tgcgttttcg cgcttacaga gcatctctac acgcttagag aggtgctcaa
     9361 aggcattcac agctaaatcc cagtgtttaa acgcctcagt tagtgccgct ggtgtatttt
     9421 ctttgtatgc ttcaagttca gcgcggttta aaggcaggag gtaatcatcc tcataataat
     9481 taattagtga gtaaattgta tcatacgtgt ttttgttcat attaaaagtc tccatagtga
     9541 tcagcattat ctagaatttg gaattctgga actaagttat ttaggccatt tgtggcgaga
     9601 taaccgacgt agtggttgta gtcttcaaat cctaaaatct ggtatttgcg taaatcagag
     9661 ctatcaacaa tatactcttt tctttttcta ggcttccaga acacgccagg tgttgaagat
     9721 aagatttctt gcaaggcgtt aaattcatct ccgcaaggca cagagggaga atgttctatg
     9781 atctctgttt tccattcgcc aggggcgcta ttttgcttgg tgagcgtaac tagctcttta
     9841 aagtctttgc gtcttagaat agatttaggt ttacttagaa gagaagctga atcaccgatt
     9901 ttcttaacaa cgttgttttt tctttttgcc atttattttt ccttttcttt taaaggaaaa
     9961 caccccgaaa tatgcttcta acgtgagaaa gaacaataac caatacatag ctactatcat
    10021 aataatgttt tccttttata ttttaattac atagagagat tttagagagt ggaatagatg
    10081 aattctattt ctttatctaa tcttttgaga ttgaatgtag agaaagataa ttctttaatc
    10141 tctataacac cgaaatttga tagctgtttc agccagaatt ttaaaccatc ttcagagcac
    10201 cctgctcggt gtaacacatc gctatatttt agcggtgttg gctttccttt atagaggtaa
    10261 gcaagtgttt cgtagatgcg ttttcctgtg tcattacact gaattctgtc tacttgattt
    10321 tttactaaat attgaatcat aaccctcctt attaaaatgg aatatcaaag tcatcataca
    10381 catttgggta atgcttagaa aaatatgcct caataccatt tggtagcttt aacctgtgct
    10441 tataggctaa cgatgttgca aattctataa atgatgctcc tcctgttctg gtattcttct
    10501 gctggcagat aagagaggca aaattctcta acacatcctc ctcattatca ttagctacct
    10561 tttcacttga tgaaacaggt gtttcttccg tctcccacgg caaaggcgca tcccaatcat
    10621 caagaacatc aaaagaaggc tgtttattat cttccgtagg gggaagctga tcaggaacag
    10681 ctttaacggt agcttcttgt tgtgtctggg cttcttgtct agcttcttct tgtggaatat
    10741 ctaccacttg atagacatta gaacttcctg gtcgtgcatc ttttataatt aatcccattt
    10801 cgagcaattt cttaaccctt tcctctgctg ctcttggtgt tatgccaaaa acatcgccta
    10861 tcttagcgta agagggaaat gcacgatcaa accctttcag atagcagtaa aggtttttag
    10921 cactatctgt aaatttatgc ccgtttactt ggcttatttt tagaatccac ataggaactt
    10981 ttacaaattg ttcttcatta atcattaaaa atctccttat aaatcacgct taatgcgcta
    11041 ctaaagtgaa tgaggatcac ttaccctttt tttgtttgat gttctctgct gcttcagtaa
    11101 tgatttctaa catcaaatca ctgaccataa tttgcctttt cttggcttca gcaaataaaa
    11161 ccttgaagat ttcagggtca acgtgtaggt aagtgcgcgg aattggtttc ttagtttcgc
    11221 tcatagtttt ctcctctcct gttattattc tttctctgct tctttctcag ctttacgcag
    11281 ttcatcaata atttttagtg catcgtcaaa acatttttca cgctctggat aaccacaacg
    11341 gcgagcggtg tttctataca tccttacttt aaagcggttg atctcaccac gtttccaggc
    11401 tgcaactaca tcctgtgaag taaaccattt cataaaaact ccttattcag ttaaaagaaa
    11461 aagacacctt gcaagccttg ggcttcaaag tgcggtgcat tggtttaatt atttagagga
    11521 taagaatcta ttcctgtccc ctctaatacc tattattgca cagaaagaga cgtttgtcaa
    11581 taccttttca caaaaaatct gatctttttc atgcacttac attttttctt agcaattcgt
    11641 gctctcctgt gtggtgctat tcctccttat tgtctaaaac gcgttctaac gagattttga
    11701 taaaaacaat acattccctt atgtagaggt tgtttagctc gccagaggct tatttaaggg
    11761 gcttattttg tatttgttag gtatatgaaa aggtgatggt agatataaga tagagagtca
    11821 ctttataaaa cgctctgtaa agctctgtag tgagcttata tgtgtaggca atgcgtttcc
    11881 attctctacc ctgcttaatg ctgttagaga agcatttaga ccccttattt ttaattacct
    11941 aaagtatcct ctatacattc ctgttatgaa acacctctga atccttcata aaacacatta
    12001 tgacgcaaag aacacctttt ctaaggtgat tcccttcctg aatgctgttt cttgcgtcag
    12061 agagctacag agagcttata aagctattat taataataaa gtagataaag taagataagt
    12121 aagaagatta actaagatag ataagattaa taagatcaga attctctttc ggtgtcgcct
    12181 ctggcttttc caccgaaaac aattacacga ctacagaatg tggaagtgga attgtattga
    12241 ggcttagtga aattacacga ggaaagcaga gtgtaattga gctttagccg ctctctcttc
    12301 gagtaattac acaactacag agaataacag agttattaaa tgtaggaggg taattaagag
    12361 ttagagagag ttaacacgta agcaatcagt atgattggtt aggtgattac tcttactgcg
    12421 cacagcgcaa tagtatttct tcgtgggcgt taccgccaac gatagttaca cgaagaaagt
    12481 aacgctttgc tttcggtgtg agcggaacgg gccagccaac gtagcccgaa aggtagcgga
    12541 gtaaggctgc ttgccagaga gtgaagctaa cgccggaagg caaaagatca tataaataag
    12601 tatataatat tataatttaa taaatgatct aataggtaac agaattctgt gggggtgtct
    12661 aacagttttc tgtgggggtg tataacagaa ttctgttagg gtggggtaac agaattttgt
    12721 tagggtacaa atctctttaa aagttaagat taacagaatt ctgtgggggt agctcacagt
    12781 gactaccgtt actaccaatc atcacatagc actcaccctg tgtaatgcct ttctatgtgc
    12841 gtgccataat ccccctatat tccctgtgta aatcatagaa tatccctaac ttattcctgt
    12901 gtaattccat actaaaaatt caatgaaaaa gtagcttaaa agttcaattc tt
//
LOCUS       DAZGXZ010000135        12876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000135 DAZGXZ010000000
VERSION     DAZGXZ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.135"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..155)
                     /locus_tag="ACHKJF_004202"
     CDS             complement(<1..155)
                     /locus_tag="ACHKJF_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951462.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCRGRLILQTD
                     AQGAWFE"
     gene            complement(152..535)
                     /locus_tag="ACHKJF_004203"
     CDS             complement(152..535)
                     /locus_tag="ACHKJF_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0951463.1"
                     /translation="MYAKSFIALDGNGHLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTELPWFEHTDDGLTEHGQQCPYVRPERREVRLIKRLQKFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCHTGHFSEEGVAE"
     gene            complement(906..1505)
                     /locus_tag="ACHKJF_004204"
     CDS             complement(906..1505)
                     /locus_tag="ACHKJF_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0951464.1"
                     /translation="MKTYNTNPGYEMDPQLLTHFNQHLDSLFGIYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEILRLINEAGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGDGYYHWCRFNKNYPVNINHVIHYSDHKAVNALRYALSYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLGSTLPERCSLV"
     gene            2782..3573
                     /locus_tag="ACHKJF_004205"
     CDS             2782..3573
                     /locus_tag="ACHKJF_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951465.1"
                     /translation="MTTRITFNMTSDETLRIVDEYCHTHKLSRSKVIDALLSATAPVL
                     NDINCYYQLAGKLQSRLLNGVYQRDLPHKRNVVSAEKYCLEIWENKLFTKRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKAKYACFIYIERTIYNHD
                     NPSGGTPVKAAVGNAVILLAKDVIYDEYFFDLRQSFFVSVKDLMASGAKGIPETQKYP
                     DVYCWIPLFSINSGVVITPVYKIDPRKPLTVKKPDQITVVCNYRE"
     gene            3959..4165
                     /locus_tag="ACHKJF_004206"
     CDS             3959..4165
                     /locus_tag="ACHKJF_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0951466.1"
                     /translation="MTTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            4259..4861
                     /locus_tag="ACHKJF_004207"
     CDS             4259..4861
                     /locus_tag="ACHKJF_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951467.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHI
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            5334..5549
                     /locus_tag="ACHKJF_004208"
     CDS             5334..5549
                     /locus_tag="ACHKJF_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951468.1"
                     /translation="MAWNTDVSADGKPHSLWKQGRALASQPESVTKTNIAENRIHRSG
                     VNARRQKPLSAQPPSDLNKKHTHNRLT"
     gene            5691..5987
                     /locus_tag="ACHKJF_004209"
                     /pseudo
     CDS             5691..5987
                     /locus_tag="ACHKJF_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            7237..8412
                     /locus_tag="ACHKJF_004210"
     CDS             7237..8412
                     /locus_tag="ACHKJF_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951469.1"
                     /translation="MWEEQVRCIVGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWL
                     EKEPRASYFFLFRLYTSIHNTFFPQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDY
                     APQPIPEMDSSVVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGM
                     PPEHLRFATGFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIIN
                     SVFSEWASINNDYPSPFSWVDSRDSGQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACA
                     TFDNWEGWNEQQVQFLLKSNPRRNRAKFTISPFPALRVKQHKAVLLDELKSAREQQKR
                     RDERADGSVPLKLSGKIHKQLESIARSRGVPPKKMLNEMIEQAHRDFVANEQHKTRS"
     gene            complement(9271..10005)
                     /locus_tag="ACHKJF_004211"
     CDS             complement(9271..10005)
                     /locus_tag="ACHKJF_004211"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0951470.1"
                     /translation="MPVLDFKEIPEAHKATGLQDTFELFSRDFLAFLGYKIISDPDRG
                     ADGGVDIIAEEKRTGVGGETIIRWLVSCKHKAHSGNSVNPGDDANITDRVIANNCQGF
                     IGFYSTLASAGLSGILDGLKGRIEFQVFDKEKIERELLHSSRGLEIAERYFPKSLSEW
                     KTENPKPARIFSDKPSLKCKVCKKELLFQDDKGIITIWQRIRTDYEKEPEYNEHIFST
                     CRGNCDQALRQYVRKQTMALLMAGKI"
     gene            complement(10181..10783)
                     /locus_tag="ACHKJF_004212"
                     /pseudo
     CDS             complement(10181..10783)
                     /locus_tag="ACHKJF_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010340155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            11015..11959
                     /locus_tag="ACHKJF_004213"
     CDS             11015..11959
                     /locus_tag="ACHKJF_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFP0951471.1"
                     /translation="MDLIMEWRFLGSLSEARKSGCSGVYLIVHKGLFSRVVYVGVSCN
                     VGRRITEHYDGYLRGNRTIYDAGHDEDVYRFMSAYKIHNHTKYYQALANDYKIWASTT
                     MYSDLPKNMLAKSQTFDTDWQSIALEKYIPQLVVWALPMAKYCYSNASRIESVIQSKL
                     IKSFDLRGFFNIKQLSILGKIEYPYMEKVKVFIINTPDLDPASQLIFSNLYNKKTDNN
                     FCKEFRSQFKSEIFQRESETQRKRTIREHKVSLYENYGKPWTLKEMEKLRVMLVDFDL
                     SPTEISEYLGREPRSISKKISENDKVTNYKWRESVVWL"
     gene            complement(12133..>12876)
                     /locus_tag="ACHKJF_004214"
     CDS             complement(12133..>12876)
                     /locus_tag="ACHKJF_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951472.1"
                     /translation="GKEFRKLFNRYETGFYLSEFEYLKDFDPLFPKCVGKELKLGYKR
                     KVDVWCEEDAALYAEFIATKERLKRGRLILKINQVWSRVYPAYIYAIEERINVRAKGR
                     ESFKYETVQEKQCKLSIMLDGLKDLECEIAMISSEFEIEKHSMHYFYMIFDIERYIND
                     CSYFVFSAAKVVKGVIQCIGYIDKNNKASCDEFYSGSYECLDDDQETLNYLNESCAEG
                     IDADKDLIRKNELLALSLKELYQLLVSKK"
BASE COUNT         3665 a         2977 c         2820 g         3414 t
ORIGIN      
        1 tgctcaaacc acgccccctg agcatctgtc tgcaaaatca accggccacg acaggaaagg
       61 cagaaacact gccggatatt tctgctgagc gcagagtcgg cactgataat gtgttcaccc
      121 ggcgtgatcc ccagccccgt ttttataccg ttcattcagc cactccctcc tcactgaagt
      181 gccctgtatg gcagtgagtg cagtactgct ccccataata atcgtgatga cattgtctgc
      241 agtgccagct ggctttacgc accacgggta aggcatccgg tacgaatttc tgcagacgct
      301 taatcaagcg tacctccctg cgctccggtc tgacataagg gcactgttga ccgtgctcag
      361 tcagcccgtc gtcagtgtgt tcaaaccagg gaagttcagt gtcgtactgc gggtggtatc
      421 tgagtgcact gccgcaaagg tggcaggtgt agtgagcata aggtgcggcc tgtgcagtac
      481 gggcgcccgt cagatgtccg ttgccatcaa gagcgataaa ggattttgcg tacatcgtca
      541 tattcctcag ccgccagacg acacgcaggc aacagccgtc ccggtacggg cagtgtggtc
      601 aggtgtgaat ggcggttcag ttaaaatctg gactggagtt tgggtaaagc aggctggttg
      661 ctgttacagt actgtggttg tcacacaatg tcccaccggc gtaaaaaact gtcagcaggc
      721 agggatggac atcagagaat gtgctgtatc ggttgccgga aggactggta aagaatgtgc
      781 catggttaaa tctccgtatg gttcatacgg aatagccatg tcgtaaaaaa tgtgcagtgc
      841 cgctgaagtg gcctccggaa ccgactgtaa cgagtagcgc cagcccgcag ctgtcgttca
      901 ggggcttaca ccagagaaca tctctccggc agggtgctgc ccagtcgcgg tcgcccccga
      961 ttactttttt caggaagccc gctgcatttc aggatgatgc cacattcttt ctgttctcgt
     1021 ttggccaggt agctgagggc atagcgtaag gcattcaccg ctttgtgatc tgaatagtga
     1081 atgacgtggt taatgtttac cggataattt ttattaaacc ggcaccagtg gtagtaacca
     1141 tcaccctcgg taacccgcct ccagatatct cccatcaatc tggataccag atacgaactc
     1201 ctgtggctct gaccgttcag atagcccaca aaatgaatgt gaagcccttt acgttccgta
     1261 tattccatta cccaggcata cccgaccagt ttctctcccg cttcgttaat cagtcgcagt
     1321 atctccgcgc acatggcgta cctgcaggca cacctgtagc tgagggtgtt ttttcggtaa
     1381 gcaaaatcca ttctgaacgg taacagtttc gaatatatcc cgaataatga atccagatgc
     1441 tggttaaagt gtgtcagaag ctggggatcc atttcgtaac cgggattggt gttgtaagtc
     1501 ttcatgtttc ttcctcgtgt tctgtaacac aggaagacgc gttgccgtaa atttgaagaa
     1561 aacgttatca ggcagggtga cctccggtaa cgacgtccac gggtatcaga agcagacaga
     1621 gaacagcata cgtaacagcc cgttacagcg catataacgc ctgaagctct ggtaaccaga
     1681 ctgaagcggg aaggcgttta taacgcgtgt gggcaatcac tttttccagt gaacaacagc
     1741 ggaactactc tggtttaaaa ttccattacc ctcccgttat cctgtactgt gattaacgta
     1801 ataccgtcag aatggcctga cgttatccct aaagcctttc cacttccctg tttctccggt
     1861 aaaaaatgct tttaatacca gccataaccc gatatgacaa ttaacgtagt tttccgcaac
     1921 gtactacata agtaacagag atacagggtt aaacaacacg ccgtaaggct gtttttaatg
     1981 gtataaggca tactctttta cgtattacta ccaaaactca tcaggtaagc tgcaggaaca
     2041 gatcattaac agtaattcct taatcaccgt tatccacagt atcaacactg gcaaagtcat
     2101 actcttattt agccagaaac acttacacaa acgcacaaaa gcaatggcgg acccagaata
     2161 gtctccacac aacaggcatt acttaacaaa gtacgttaca gtcagtgtgt gtacattaca
     2221 gatttatatt caggtgttac gggattacat tgcagcagag agggttaagg acagtgttac
     2281 aagtactaca gagaatatcg ctgcacagat acttctatga gacagacttc tgcaggacct
     2341 ggttacaagg tgtatttacg aggtacaatt acaggggaga gttacaggat agaattacag
     2401 gatagaatta cagagttcat ttacacggta gaattacagg gtcatttaca tgaccatact
     2461 tctataaata atactaatta accttaatta ttttttaatg gtacatgaac tgcctgtagg
     2521 tgtaacgata aacaaatcac ccaaaacgaa aaatactggt tatcatggtt cagagaactc
     2581 atacaagtgg cagctccccc cctgagcaat gcttcatttt cggtggttct gtgtgataga
     2641 gttatactga ctacagtggc agtaacactg ctttccacca cactaggata aagccagtaa
     2701 aaaaatcaat tatatgaatt aaaagcattt tttatgaaat gttatgcata tcaaccagta
     2761 tgattaacag aaaatttcat catgacaaca cgtataactt tcaatatgac cagtgatgag
     2821 acgctgcgaa ttgtggatga gtactgtcat acccacaaac tttcgcgctc taaggtaatc
     2881 gacgcattac tcagcgccac agcacctgtc cttaacgata ttaactgtta ttatcaactt
     2941 gcaggcaaac tgcagtcacg cttattaaat ggagtgtatc aacgggatct tcctcacaaa
     3001 cgcaatgtcg tcagtgcaga gaagtactgt ctggagatct gggaaaacaa acttttcacc
     3061 aaaagaatcc tggagtttga cagttcaaat ggtgttctgt acgcgctgaa acataaaaga
     3121 cactatcgcc gggacaagat gattggaagg gttgaaagcc ggtgtatcaa agacatttgc
     3181 gaataccaga tgcaactgtc cggggagaag gcaaaatatg cctgttttat ttatatcgaa
     3241 agaacaatat acaatcacga taatccatcc ggtgggacac ctgtaaaagc ggctgttggt
     3301 aatgcagtca tcctgctggc aaaagacgtg atatatgacg agtacttttt tgatctgcgg
     3361 cagtcatttt tcgtgagcgt taaagatctg atggcctctg gcgcaaaagg tattccggag
     3421 acacagaaat atccggacgt ttattgctgg atcccgcttt tctccataaa ttccggcgtg
     3481 gtcattaccc cggtctataa aattgatccc cggaaaccct taacggtaaa aaaaccggat
     3541 cagatcactg ttgtttgtaa ttacagagaa taatttcagt cttctccgtt caccgcaaca
     3601 gtgtttacct ctgctttatc ttccgttcag taaatacaaa ccaccgctct ggcaggtaca
     3661 gggcgtggtt tatctccgat actcccctgc gcagtaataa tcctcactga aaatactgat
     3721 cccgtcacgg caaaacgcca tcccatattc cggcaacaca aaatcatctt ccgtttatac
     3781 cggcttcact gacaaaacag acaacctgta ttgcctgtac acaacagttt ctctcctggc
     3841 aggaatattc cccctactga ttttttacgt ttctccccgg ttgttatgcc ggtgaaggtg
     3901 gtgcgttgtt ttcatcatca acgccggttg agattaacaa catccggagg agatttttat
     3961 gaccacccca gtttcgctga tggatgacca gatggtcgac atggcgttta tcactcaact
     4021 gaccggcctg accgataagt ggttttacaa gctcatcaag gatggggcct ttcctgcgcc
     4081 catcaaaatg gggcgcagct cccgctggct gaaaagtgaa gtggaagcct ggctgcaggc
     4141 gcgtattgca cagtcccgtc cgtaattttt aaccactaac ccttcgccca cagcagacgc
     4201 aatctccggc ctgccggcgg cactctgctg cctgttatcc cccgtgagga atatgaaaat
     4261 gaaacaacag tatcagaccc gttacgagct gctccatgaa aactaccaga aatggctgac
     4321 cggcttcacc cggcacgccg tatcatgggg cgtgtgtcat ccgaatatct actatttcca
     4381 taatctgacg cccgggtggg tgtcattcaa tggcgaaaaa ccggagattg ccatcgttcc
     4441 ccagagtcta caccggctga tttatggtcc ggacaaacgg agctccccgt ccctggatga
     4501 tgatctggtg gtgaatttat gcaccagcga acacctgctg gttcatcatc cgatgctgga
     4561 agggatcctg ctgtctgaat gtgagcgcct gaagcaacat tctctggcaa acaaactgat
     4621 cagcctcttc cgtcagtttg gcggcacgga gttacgtctc aaactggtct ggctctgctg
     4681 gcttgactta atgaccggaa acagccttga cgactggacg aaaaacctga aacacatatc
     4741 agaaaaagat ctggagcaat ggatcattgc acgtcagggt cagagtgaac cactgacaaa
     4801 tctgatggat cagtacgtgc ttatggcata ccgtacatcg gttgatgccg ctcacagttg
     4861 atgtctgaga accacagggc tggcagcaga aaaccagccc tgtggtaaaa tacagtaata
     4921 gcggcgtaca tcaggcacgc cgctcactcg tccacagatc atatacgtca ttatactgcg
     4981 cgataacgga gggcttcagc acccggtcat ttctgagcgg gtataaatac cattgatgat
     5041 gcgaagcagc cacgggcaga aacgttcttc acaaccaggt gcctccatct tcactgcagg
     5101 ctgaatgtgc cggcttgtta tccaccagaa atggtatact ccggtacatg cataccccgg
     5161 agactataaa cgcactgaat catagcctcc cacctcagca ggaggcagtc agctttcagg
     5221 cggaaaaacg ttgcagttaa agatcgtgag aatcgctgtt tcgttgcata gcaacccgaa
     5281 cagttgtagt gaaacatttt acctgcatca gcacatggtc aggcgccgtc tgtatggcgt
     5341 ggaatactga tgtttcggct gatggcaagc ctcattctct gtggaaacag gggcgcgcat
     5401 tagcttctca accagagagt gtcacaaaga caaatattgc tgaaaatcgc attcatcgtt
     5461 caggcgtgaa tgcccggaga caaaagccgc tgtccgcaca gcccccctct gacctgaaca
     5521 aaaagcatac acacaacagg ctcacctgac cccgaaaaac cataacttca gcaataccag
     5581 tgacaccagc aagttacccc agtgccacat cttcagaccg cgatcaatct gaaatgtgac
     5641 tatcggggca atatccctgc tttgtggtac acgataacca gaaaacatcc cctgaaccgc
     5701 cagccagaaa gactggccac aaaaggggga agctgaaatc gtttatggat tacatcgaca
     5761 tatgcctggc agagaatgtc cggaatgacg aggttatctt tgcggagatt aaggcaatgg
     5821 tatacgggcc gacgttccat gccgcgttac tacattcagc ccggacgtaa aatgcgcccg
     5881 tcaaaaagaa cggttcgctt cgaaactcag ccaggatacc acctctccgc cacatccggg
     5941 aagaagttga tatggtggtt gccggacaac agcgctcctg gattgactgc tgcatcactc
     6001 aaccacgctg aatatcaaag gagagcgtta ccggttaaaa gagaaacgta aagctgtggt
     6061 gatgacccaa aacgcaacgc ctgtcagtga ggatgaaatg gtgaaaagcg gacagcatca
     6121 gtaacggaag tatcttagcg ggtacgaaaa tggcaggatc gtgacattga cagaatatct
     6181 cctgatcatc ctgaatgact gcagttgctg tggaaaagta agctgtctcc acccacggca
     6241 ccacttaaca ggcattccgt aactaccccc agacttacta tgacgcatta tcggtgcact
     6301 gaaaacagga gagtgcgctt aaaaccgcag accgcattgc gggtgttact gcatgacagt
     6361 ctggccgtat tgtgagatgt cggcaggaac cgatgtccgc gcagggggaa cttcctgcag
     6421 tacctgacgc ttcaaatggt attaaccgta gggcgttgtt ggttccggat cgcggaaatg
     6481 ggagcacagg tctgctacgg ggtgtacctc tgacagtaaa tccggctcgc tcatctgact
     6541 gcctctctcc cggaaaagac caaaaatgag atcgtctcct caacagcaga gaagaggtct
     6601 tgacagacag gctacattgc atacgattta tgaagaaaaa gacagcaaat atggaggtta
     6661 tagcggagaa caccctacga aattcctatg aaaaatgatt gaaaaaaata tcaaattcga
     6721 ttcgttttta tatgcttttt gggaacgtgg cgatgacgaa ctgttgcatg tgcagaggag
     6781 aatgcacccg gaagcaccag aatcatccag aaaggcagaa cgattacgca tcaaactagg
     6841 agacagcaat atcaaggtaa tgtactgatt taaaatgaat ttacagcaat acgtgaaaac
     6901 attttttact taaaggagac agatgcgctg aagagagtgc aggtattttt atataaaaat
     6961 gagaagttta cgggatgtat atccggcaca ctatgaccgg agaatatcac tcaagcttaa
     7021 aaagataata aatacagtga gttaaataca cagagaaaac gtaatatcag agggaacatc
     7081 ttacaacaga gactgaattt ccggtttttc ataaccggcg cattccggaa agtgcaggcg
     7141 gcccggaggt cttctgacgc cagtacctga tagattgttc tgtacattct gtacatcatg
     7201 tacggaggtt tttgtttgct ttcttaaaat ctgcaaatgt gggaagagca ggtgaggtgt
     7261 attgtgggaa aggaactgga tgatatctgc accagctgtc cgtatattga tgctgtaaaa
     7321 cggcacaaac aacagctcgg tgccatagag gaatatacac agtggttaga aaaagaacca
     7381 cgggcatctt atttttttct gttccggctg tataccagca ttcacaatac atttttcccc
     7441 caaaagcaac aactcccgtt cactcccggt ggcactcact gcccggaacc tgatgtcacc
     7501 cttcgggact taacgctttc cccgggctac cacagtgatt atgcccctca acccattccg
     7561 gagatggaca gttctgttgt tgttcccccc actaatgaaa acacctcgcc tccggaagat
     7621 actcccgaca acactccggc cggaggcaat accggacaag cggaaaagac cagaaacgca
     7681 ggactcacac ccattcctga aaaacgttca ggaatgcccc cggaacatct gcggtttgcc
     7741 acggggtttc catcgcaacc gaagattgcc ggtcccagag aaaagccgat gcgcaccgtg
     7801 catccggata aaatctaccg tgaaattata tggttctgct ccagttacct gctgaaatcc
     7861 ggaccagagg ctacgaggac gattatcaac tcagtattct ctgaatgggc cagcataaac
     7921 aatgattacc cctccccctt ttcgtgggtg gacagcaggg acagtggaca gtgtgactgg
     7981 ttatggaacg ccatgcaggt ccggtgtgtg ggaaccccgc taaatcccct taccccggag
     8041 cagaaatact ggttcgcctg cgccacgttt gataactggg aaggctggaa tgagcaacag
     8101 gtacagtttt tactgaaaag taatcccaga cgaaacagag cgaagtttac gataagtccc
     8161 ttcccggcac tcagggttaa acagcataaa gctgttcttc ttgatgagct gaaaagtgcc
     8221 cgggagcaac aaaaaaggcg tgatgagcgt gctgatggtt ccgtcccgct gaaactgtcc
     8281 ggaaaaatcc acaaacagct tgaaagtatt gcccgaagtc gtggtgtccc cccaaaaaaa
     8341 atgctgaatg aaatgattga gcaggcgcac cgggattttg tggcaaatga gcagcataag
     8401 acccggagtt aaagacggca gggtccccga ttaccaattc tgcagcgtac tcaacaaatg
     8461 tatccggccc ttacctgtga gggacacgaa aattatacat tgagcatgag tggataatcc
     8521 cgggtgagag tctcccgttt ttacactatt cactaaaggt gaggcatatc attcagtctc
     8581 tctgaattct tcatttcagt aacttcagct accgtcgttt catctctccc acgaaccgga
     8641 gccgcaactt gttgcatcac agggctgaca agtgaactga aatccgcacc ttaatattct
     8701 gactggctgc gtacattcat tgcatgaagc ggccggagcg gctgagctga aggtccgttg
     8761 tgagcgaaaa gcggacatta actccttgct ttttggcact gaaacagagc gaagcagcat
     8821 gtaagctgtt acggatgcgc agcggtgaca acagcaactt caattaagga aatgctcttg
     8881 gttttcgggg agacttttcg agcgatatat gataatgaag acactaaaaa actgaacaga
     8941 aacgtctagc tcaatatgac ttctgttcag gtataaatgg tctatttatg tattctctca
     9001 tcaaatcctt actgaatact ctcgaagatg gctaacgccc cctctcttaa ccttcatagt
     9061 tcatgccgcc aagatacgaa ggtatcatca ttaatgattt aaccctttct ttttcttttt
     9121 cggtgagatg acgacatgca aatgggtaaa tattgatcaa aaatgtcttg agttcgtcaa
     9181 atgcctcatc tgaatataga gtcccagatc ttaactcatt cattattgtc atgacccatt
     9241 ttataaacac agttggcatc attacatcag ctatatcttc ccagccatca ataaggccat
     9301 ggtttgtttt ctaacatact gccggagtgc ttggtcgcag ttgcctcgac acgtcgaaaa
     9361 aatatgttca ttatactctg gttctttttc gtagtcggtg cggattcttt gccatatagt
     9421 gataattccc ttgtcgtctt gaaacagaag ctctttcttg caaactttgc attttaagct
     9481 aggtttatcg gaaaatattc ttgcgggttt ggggttttct gttttccact cagataacga
     9541 ctttgggaag tacctttcgg caatttccaa tcctctggaa gagtgtaaaa gttccctctc
     9601 tattttctct ttatcgaaaa cctgaaattc aattcgtcct ttcaacccgt caagtattcc
     9661 agacagacct gcactagcaa gagttgaata gaacccgata aatccttggc agttattcgc
     9721 tattactcga tcagtaatat ttgcatcatc tcctggatta actgaatttc cactatgagc
     9781 tttatgctta cagcttacca accaacggat aattgtttct ccgccgacac cagtcctttt
     9841 ttcttcggct ataatatcaa ccccaccgtc agcccctcta tctgggtcag atataatttt
     9901 atatcccaga aaagctaaga agtcgcgaga aaaaagttca aatgtgtctt gcagccctgt
     9961 tgctttatga gcctctggta tttctttgaa gtctaatact ggcattttta tggggtcctt
    10021 aatttgttaa tgcttattgg gtggaaagaa ccgtatttaa acacggactt tctgcctctg
    10081 taaatttaat atgtagaaca ctcatcactt tttataactg acaataggat atttagacgt
    10141 ctgtttttgg cacagggctg ctgttgcagc cctgacaacc ttagacttcg atcgattcag
    10201 agatctctaa tgcatcatcg acttcaatgc ccagataacg gactgtgctt tccagtttct
    10261 tatggcccaa cagaagttgg atcacccgga gattcttggt tttcttgtat atcaggtaag
    10321 gttttgttct tctcatggag tgtgtgctgt aaagagaagc ttccagacca agtttttcta
    10381 ctcatccatg aaaaattcgg ttgtattgcc gggttgatat gtgccggtta gttccgatcc
    10441 gagaccgaaa caagaagtcg ttgctatgca aatttccaag ttttatcaat gcagcaacgg
    10501 cttctcttgt ccctttggtt atctcaaatt gcacaggact accggttttc tgttgcagca
    10561 cagttgctct gctggaaacc gagctaccat atgcaacatc agatactttg aggttgacca
    10621 gatcacagcc acgtagctta ctgtccaaag ccatattgaa cagagccaaa tcgcgcgtct
    10681 taccttctag ttcaagcctg attcggattc cccagatatg ggatatctga agtggtcttt
    10741 tttggccgac gatacggtct ttgttccacg gtgacgtgtt catactcgaa tctcctgcaa
    10801 tatgggagat ttgagtatgg ttgtctctca tgagcgagga acggacatta ctaacagcat
    10861 tctgtgtgaa tcaccaggga gcaggtcagc gccactactc tatataatcg ctaacgatga
    10921 caagtataat tatataagtc tttgccctta gcttttggat aaaattgttg aagttttacg
    10981 aaaataatga taaacaaaaa aagtagcaat aataatggat ttaattatgg aatggagatt
    11041 tttaggttct ctatcagagg ccaggaaatc tggctgtagc ggagtttatc ttattgtcca
    11101 taaaggactt ttcagtcgtg tggtttacgt tggggtaagt tgtaatgtag gtagaaggat
    11161 aaccgaacat tacgacggtt atcttagagg aaatcgaaca atttacgacg ctggtcacga
    11221 cgaagatgtt tatcgcttca tgtcagctta taaaattcat aaccacacaa aatactatca
    11281 agccttggcg aatgattata agatatgggc atctaccaca atgtattctg atttgccgaa
    11341 aaacatgcta gcaaaaagcc aaacctttga tactgattgg cagagcattg cattagaaaa
    11401 atatattcct caactggtag tatgggccct tccaatggca aagtactgtt attcaaatgc
    11461 aagcaggatt gaaagtgtaa tccaatcaaa gctcattaaa tcttttgatt tgagaggttt
    11521 ttttaatata aagcaattaa gcatattggg taaaattgaa tatccctata tggaaaaggt
    11581 caaagttttc attattaaca cacctgattt agacccggca tcccagctta tttttagcaa
    11641 tctatataac aaaaaaactg ataacaactt ctgtaaagag ttccgttcac aatttaaaag
    11701 cgagattttt cagagggaaa gtgaaactca gagaaagcga actatcaggg agcataaggt
    11761 atctctttat gaaaattatg gcaagccttg gactttaaag gaaatggaaa aacttagagt
    11821 catgcttgtc gattttgatt tgtccccgac agaaatatct gagtatctag gaagagagcc
    11881 gcgctctata tctaaaaaaa taagcgaaaa tgataaagtt actaattata aatggcgaga
    11941 gagcgtcgtc tggttgtgac gttacgtttt gattgaaata accctccatt ttgcaaagtc
    12001 cgctcttggc acagtgcgga caagctcact gagctaaagg tccgcagtga gcgaactgcg
    12061 gacatcgtgg agacaacatg ctgctgggtg cggatatccc atggcacgtc atatcgctcg
    12121 ccgtattact tgttattttt ttgaaacgag aagttgatac aattccttca gggataaggc
    12181 taataactca ttcttcctga tgagatcttt atcagcgtct ataccctctg cgcatgattc
    12241 atttaaatag ttcagtgttt cctgatcatc atccaagcat tcatagctgc ctgaataaaa
    12301 ttcatcgcaa cttgctttgt tatttttgtc aatgtaacca atgcattgaa tcacgccttt
    12361 aacaacttta gcagcagaaa aaacaaagta actgcagtca tttatatagc gttcaatatc
    12421 aaaaatcata taaaaatagt gcatagagtg tttttcaatt tcaaactcac ttgaaatcat
    12481 agctatttca cattcaaggt ctttcagacc atctaacatt atggaaagtt tacattgttt
    12541 ttcttgcaca gtttcatact taaatgactc tctccccttt gccctgacat ttattctttc
    12601 ttcaattgca taaatataag caggatatac acgcgaccac acttggttta tttttaggat
    12661 taaccggcct cttttcagcc tttcttttgt agctataaat tctgcatata gcgcagcatc
    12721 ctcttcgcac catacatcaa cttttcgttt ataacccaac tttaattctt tccctacgca
    12781 tttaggaaac aaaggatcaa agtcctttaa gtattcaaac tcacttagat agaatcctgt
    12841 ctcataacga ttaaataatt tcctaaactc tttccc
//
LOCUS       DAZGXZ010000136        12816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000136 DAZGXZ010000000
VERSION     DAZGXZ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.136"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            217..1731
                     /gene="caiT"
                     /locus_tag="ACHKJF_004215"
     CDS             217..1731
                     /gene="caiT"
                     /locus_tag="ACHKJF_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP0951473.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1762..2904
                     /gene="caiA"
                     /locus_tag="ACHKJF_004216"
     CDS             1762..2904
                     /gene="caiA"
                     /locus_tag="ACHKJF_004216"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP0951474.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3032..4249
                     /gene="caiB"
                     /locus_tag="ACHKJF_004217"
     CDS             3032..4249
                     /gene="caiB"
                     /locus_tag="ACHKJF_004217"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP0951475.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4323..5876
                     /gene="caiC"
                     /locus_tag="ACHKJF_004218"
     CDS             4323..5876
                     /gene="caiC"
                     /locus_tag="ACHKJF_004218"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP0951476.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5985..6770
                     /gene="caiD"
                     /locus_tag="ACHKJF_004219"
     CDS             5985..6770
                     /gene="caiD"
                     /locus_tag="ACHKJF_004219"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP0951477.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6776..7366
                     /gene="caiE"
                     /locus_tag="ACHKJF_004220"
     CDS             6776..7366
                     /gene="caiE"
                     /locus_tag="ACHKJF_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP0951478.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7452..7847)
                     /gene="caiF"
                     /locus_tag="ACHKJF_004221"
     CDS             complement(7452..7847)
                     /gene="caiF"
                     /locus_tag="ACHKJF_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP0951479.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8108..11329)
                     /gene="carB"
                     /locus_tag="ACHKJF_004222"
     CDS             complement(8108..11329)
                     /gene="carB"
                     /locus_tag="ACHKJF_004222"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP0951480.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11347..12495)
                     /gene="carA"
                     /locus_tag="ACHKJF_004223"
     CDS             complement(11347..12495)
                     /gene="carA"
                     /locus_tag="ACHKJF_004223"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP0951481.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         3033 a         3316 c         3381 g         3086 t
ORIGIN      
        1 acaaactgat taataatcaa aattaacgag aaaagcgccc tctattcacc cgaaacaaaa
       61 atgtgataac aatcacagaa tgcagcttat tgaataccca ttatgagtta gccattaacg
      121 cgtccacgag gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
      181 caaaataaat gttcgttttc acagtggaat taaatcatga agaatgaaaa gagaaaaacg
      241 ggaatagaac cgaaggtttt ctttccgccg ttaataatcg tcggcatact ttgttggctt
      301 acagtcagag atctggatgc agcgaatgtc gttattaatg ctgtattcag ttacgtcacc
      361 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
      421 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccacctga atttagtacc
      481 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
      541 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
      601 ggggcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
      661 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
      721 attcgcccca gctcgacact ggtgccgctg gtaggtgaaa aacacgccaa agggttgttc
      781 ggcactatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
      841 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
      901 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
      961 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
     1021 atgctgggtt gggtgttcat tgtcagcggt gccagcttca tcatgaacta cttcaccgat
     1081 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
     1141 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttatgct
     1201 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
     1261 ttcggcatgg tgctggggct gacagcgtca acctggatcc tgtggactgt actcggtagt
     1321 aacactctgc tgttgataga taaaaacatt atcaacattc caaatctgat cgaacagtac
     1381 ggtgtggcgc gcgccattat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
     1441 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
     1501 accctggcga tgtccacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
     1561 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttttgctggc gctcggcggc
     1621 ctgaaaccga ttcaaactgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
     1681 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
     1741 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
     1801 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
     1861 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
     1921 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
     1981 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagtt gccgggcggg
     2041 ttcaacacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
     2101 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
     2161 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
     2221 tgttttatta ccagcagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
     2281 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
     2341 gtgaccaaac ttgaaaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
     2401 gacgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
     2461 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
     2521 tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
     2581 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
     2641 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
     2701 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
     2761 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
     2821 ctgcgtgtag accgcgtctc cgggggatct gacgaaatgc agatcctgac gctgggtcgc
     2881 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct aatgcgtctt
     2941 atcaggccta cgggagcacg aatgtaggcc ggataaagcg tttacgccgc atccggcagt
     3001 catgcgctac ataatttttc aggagctaat aatggatcat ctacccatgc cgaaattcgg
     3061 gccgttggcc ggattgcgcg ttgtcttctc cggtatcgaa atcgccgggc cgttcgccgg
     3121 gcaaatgttc gcagaatggg gtgcggaagt tatctggata gagaacgtcg cctgggccga
     3181 caccattcgc gttcaaccga actacccgca gctctcccgc cgcaatttgc atgcgctgtc
     3241 gttaaatatt ttcaaagatg aaggccgcga agcgtttctg aaattaatgg aaaccaccga
     3301 tatcttcatc gaagccagta aaggtccggc ctttgcccgt cgtggcatta ccgatgaagt
     3361 actgtggcag cacaacccga aactggttat cgctcacctg tccggttttg gtcagtacgg
     3421 caccgaggag tacaccaatc ttccggccta taacactatc gcccaggcct ttagtggtta
     3481 cctgattcag aacggtgatg ttgaccagcc aatgcctgcc ttcccgtata ccgccgatta
     3541 cttttctggc ctgaccgcca ccacggcggc gctggcagca ctgcataaag tgcgtgaaac
     3601 cggtaaaggc gaaagtatcg acatcgccat gtatgaagtg atgctgcgta tgggccagta
     3661 cttcatgatg gattacttca acggcggcga aatgtgcccg cgcatgagca aaggtaaaga
     3721 tccctactac gccggttgcg gtctgtataa atgtgccgac ggctacatcg tgatggaact
     3781 ggtgggcatt acccaaattg aagagtgctt taaagatatt ggcctcgcac atctactcag
     3841 tacgccggaa atcccggaag gcactcagct tatccaccgt atcgaatgcc cttacggccc
     3901 actggtagaa gaaaaactcg atgcctggct ggcggcacat accatcgcag aagttaaaga
     3961 acgctttgcc gaactgaata tcgcctgtgc caaagtactg accgtaccgg aactggaaag
     4021 caatccacag tatgttgccc gtgaatcaat cactcagtgg caaacgatgg atggtcgcac
     4081 ctgcaaaggg ccgaacatca tgccgaaatt caaaaataac cccggacaaa tctggcgcgg
     4141 aatgccctca catggcatgg acacggctgc cattttgaaa aatatcggct acagcgaaaa
     4201 cgacattcag gagttggtca gcaaaggtct ggccaaagtt gaggactaaa cgcgtggcgt
     4261 cagtcggctt gcctgactag cgcccgccca ataacaaacg aacaaaaatg gatagaggtg
     4321 caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctt gcggacgttt
     4381 acggtcataa aacggcgctg atttgtgaat ccagcggcgg agtcgttaac cggtatagtt
     4441 atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatacg ctggggattc
     4501 gcaaaggcga caaggttgca ctacatctcg acaactgccc ggaatttatc ttttgctggt
     4561 tcgggctggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttacgcgagg
     4621 aaagcgcgtg gatcctgcaa aatagccagg cgtgcctgct ggtgaccagt gcgcaattct
     4681 atcctatgta tcaacagatt cagcaggaag atgccactca attgcggcac atttgcctga
     4741 cagatgtggc acttcccgct gatgatggcg tgagttcgtt tactcaactg aaaaatcaac
     4801 aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg gaaattctct
     4861 tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct
     4921 tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg
     4981 taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg
     5041 gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggtgcaga
     5101 agtaccgcgc caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtgcagc
     5161 cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt
     5221 cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg
     5281 ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa cgacgctggc
     5341 cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc
     5401 cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct
     5461 tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc
     5521 tacataccgg cgataccgga tactgcgacg aagagggctt tttttatttc gtcgatcgcc
     5581 gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca
     5641 tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat tcgattcgcg
     5701 atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat
     5761 ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca
     5821 gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga
     5881 aacagcaggg aactaccctg ccagctaata atcacgctat aaaacaatat gcattttttg
     5941 cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt gaatcattac
     6001 atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa gcgaatgcta
     6061 ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc
     6121 aattacgtgt cgccattatt accggtgccg gagagaagtt cttttccgcg ggctgggatt
     6181 taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggatttgcgg
     6241 gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac ggctatgcct
     6301 ttggcggcgg tttcgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca
     6361 gcttcgccct gccggaagcc aagctgggca tcgttcctga cagcggcggc gtgctgcgtc
     6421 taccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc agacgaatgg
     6481 gcgcagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga
     6541 tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgccccgctg gcgattgcgg
     6601 cgctgaaaga gatcttccgc accaccagcg aaatgccggt agaagaagcg tatcgctata
     6661 ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca ttcggaggat gccattgaag
     6721 ggccgctggc gtttgccgag aagcgcgatc cggtgtggaa aggacgttaa caaccgtgag
     6781 ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag
     6841 tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc
     6901 actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg
     6961 cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca
     7021 cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt
     7081 gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga gctttgtcaa
     7141 agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg
     7201 cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct
     7261 tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa tggaggaaaa
     7321 tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg
     7381 acctggttgc tggataagat gctacaacat cgcatctggc aagcaggacg cccggtatca
     7441 acgagataaa attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
     7501 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta
     7561 tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc
     7621 agtcgctggc actggcatcc ccgtccttcc agcttattag ggatcatctt aacttcgcag
     7681 cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
     7741 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
     7801 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
     7861 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
     7921 attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct
     7981 tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc
     8041 tcatcagaga accgtattac ttattcgatc aaattagcgg atgaaaaata tccgccatga
     8101 cacgctatta tttgatctgc gcgtgcattt cctgcaccga aattactttt tcggtcgcgt
     8161 cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt
     8221 tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcgcga cggcctgaag
     8281 tggtgttgat gatgtaggta tattcgccat tcttgatgcg gtcctgaatg tgcggacggc
     8341 cttcatgcac cttgttcacc agacgcggat taatgcccgc ttcgcccagc acaatcgccg
     8401 tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca
     8461 cgcgttcttt atcgccttcg cgcacggaaa gcagcgcacg accgtgtttc ttcatggtgg
     8521 agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc acgcccatca
     8581 cttccccggt ggagcgcatt tctggcccta acagcgggtc aacacccggg aatttattga
     8641 acggcagcac cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct
     8701 gctcagccag cgatttgcca gccatcacac gcgccgccac ttttgccagc ggtacgccgg
     8761 tagctttgga gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga
     8821 cttcgttgtt tttcaccgca aactgcacgt tcatcagacc gcgcacctgc aattcgaagg
     8881 ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg
     8941 gcagagaaca tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc tccatgatgc
     9001 cgccaatcag caccatttcg ccgtcgcaga tggcatccac gtcaacttct accgcgtcat
     9061 caaggaagtg atccagcaac actggcgcat cgttagacac gctgaccgcc gtctggaagt
     9121 agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag
     9181 acggacgtac caccagcggg tagccaatct ctttcgcctt ctctaccgcc atttcaatag
     9241 cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc
     9301 gttcacggtc ttctgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag
     9361 cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc
     9421 ctttcggctt ctcaatacgc acgatttcta gcacatcttc cagagttacc ggctcgaagt
     9481 agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc cgggttacag ttaaccataa
     9541 tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg tacacagcag tagtcgaatt
     9601 cgataccctg accgatacgg ttcgggccgc cgccgagaac catgattttt tcacggtcgg
     9661 tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg
     9721 cgaactctgc cgcacaggta tccacgcgct tataaaccgg gtgcaggtca tactggtcgc
     9781 gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt tgccagacgc gcatcggcaa
     9841 agcctttgcg tttcagctgg cgcaggaatt cagcgttcag gccagtgatg cccacttccg
     9901 ctactttctc ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatat
     9961 tggtcaggtt gaacacgccg tccacagaca ggcccgcacg gaaggcatcg gcgatgtacc
    10021 agatacgctc tgcgccagca tctttcagtt cgcgacggat tttggttaac gcttccgggt
    10081 catcgaggct cactttcggg tcgaatccag tcgcaccgac ttccaggccg cgcagcgctt
    10141 tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct
    10201 gagtggtcag acggtcgtta gcaccagcga atttttcaaa gttgaagcga ggaattttag
    10261 taaccacata gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt
    10321 tcatcagttc gtcgagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga
    10381 aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa
    10441 caatcaggcg accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa
    10501 cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg
    10561 tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt atggatgccc atcgcatcaa
    10621 agttttcgat agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct
    10681 cgtactcttt ccagccgatc agcgactcat caatcagcag ctctttggtt ggggagagat
    10741 ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata ccgccgccgc
    10801 taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaaccg
    10861 ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct
    10921 tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcggtcgcac
    10981 caatcatagt cacgccgaac tcttccaaca cgccctgacg ctccagctcc agcgcgcagt
    11041 tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc cgggcgctct ttttcaataa
    11101 tcttgcgtac tacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt
    11161 cggtcatgat ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcgcgca
    11221 gggctttaca tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg
    11281 ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac
    11341 tcctgattac ttagcggttt tacggtactg ctcaattaac tcgataaagt ggtcgaacaa
    11401 cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga ccctggaagc tgaacgccgg
    11461 tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg
    11521 caggtttgca ggtaatgttg cttcatccac cgcaaaaccg tggttctggg cggtgatcat
    11581 caccacgttt ttctccacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat
    11641 tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc agatgccgaa
    11701 taccggaata tcggtttcga ggaatttctg gatggcggta atggcgtaat cgcacggggc
    11761 cgggtcgcca ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc
    11821 cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag
    11881 gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt
    11941 tttcgcttct ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc
    12001 tgcggtggtc acttcttttg ccagatccat gccattcaga cctgggaacg cgcgggcttt
    12061 ttctaacgcc aacgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc
    12121 tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt
    12181 atggcgcttc aggtaagaag agaggtcttc ggtattacgg aagttgctgg caatcagcgg
    12241 cagatcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt
    12301 agtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg
    12361 atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc
    12421 cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag
    12481 cgctgactta atcaaaacac cctccagaga atattcactc actttatttg catattaatt
    12541 cacaatgatg acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg
    12601 cattctggag atatgggcgc taaaagtcaa cttaatggcg ggatttttta tattattttg
    12661 tggctcatgc atgttttaca ggcattaagc gacaaaaaga tcatataacc tgttctggaa
    12721 aacgattgcg cagcaagaag attgcatgaa ataaaagcga gcagggaaaa gtggaccaaa
    12781 tggtcaaaat ttacattaaa acaacaaaaa ccgaag
//
LOCUS       DAZGXZ010000137        12781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000137 DAZGXZ010000000
VERSION     DAZGXZ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.137"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..488
                     /locus_tag="ACHKJF_004224"
     CDS             <1..488
                     /locus_tag="ACHKJF_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0951482.1"
                     /translation="IANIRRDYLHKVTTAVSKNHAMIVIEDLKVSNMSKSAAGTVSQP
                     GRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKE
                     NRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQAT
                     A"
     gene            complement(528..1742)
                     /gene="ydcO"
                     /locus_tag="ACHKJF_004225"
     CDS             complement(528..1742)
                     /gene="ydcO"
                     /locus_tag="ACHKJF_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP0951483.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSGAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1795..2331
                     /gene="sutR"
                     /locus_tag="ACHKJF_004226"
     CDS             1795..2331
                     /gene="sutR"
                     /locus_tag="ACHKJF_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP0951484.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2404..4365
                     /gene="rlhA"
                     /locus_tag="ACHKJF_004227"
     CDS             2404..4365
                     /gene="rlhA"
                     /locus_tag="ACHKJF_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP0951485.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     HRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(4457..4687)
                     /gene="yncJ"
                     /locus_tag="ACHKJF_004228"
     CDS             complement(4457..4687)
                     /gene="yncJ"
                     /locus_tag="ACHKJF_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP0951486.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4909..5085
                     /gene="hicA"
                     /locus_tag="ACHKJF_004229"
     CDS             4909..5085
                     /gene="hicA"
                     /locus_tag="ACHKJF_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP0951487.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5131..5547
                     /gene="hicB"
                     /locus_tag="ACHKJF_004230"
     CDS             5131..5547
                     /gene="hicB"
                     /locus_tag="ACHKJF_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP0951488.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5626..7032
                     /gene="ydcR"
                     /locus_tag="ACHKJF_004231"
     CDS             5626..7032
                     /gene="ydcR"
                     /locus_tag="ACHKJF_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0951489.1"
                     /translation="MKKYQQLAEQLREQIATGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKEPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLCRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            7277..8422
                     /gene="ydcS"
                     /locus_tag="ACHKJF_004232"
     CDS             7277..8422
                     /gene="ydcS"
                     /locus_tag="ACHKJF_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP0951490.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDATVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8440..9453
                     /gene="ydcT"
                     /locus_tag="ACHKJF_004233"
     CDS             8440..9453
                     /gene="ydcT"
                     /locus_tag="ACHKJF_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0951491.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDPLMAENLCGMTGSFALRPEHIRLNIPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9454..10395
                     /gene="ydcU"
                     /locus_tag="ACHKJF_004234"
     CDS             9454..10395
                     /gene="ydcU"
                     /locus_tag="ACHKJF_004234"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0951492.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIILIALYLAFVKRLGAFDAL"
     gene            10385..11179
                     /gene="ydcV"
                     /locus_tag="ACHKJF_004235"
     CDS             10385..11179
                     /gene="ydcV"
                     /locus_tag="ACHKJF_004235"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0951493.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDSVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11201..12625
                     /gene="patD"
                     /locus_tag="ACHKJF_004236"
     CDS             11201..12625
                     /gene="patD"
                     /locus_tag="ACHKJF_004236"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP0951494.1"
                     /translation="MQHKLLINGELVTGEGEKQPVYNPATGDVLLEIAEASVEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AVVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3104 a         3133 c         3435 g         3109 t
ORIGIN      
        1 gtatcgcaaa tatccgcagg gactaccttc acaaagtcac aacggccgtc agcaaaaacc
       61 acgcaatgat agtcattgag gatttgaagg tcagcaacat gtcaaagtca gcagcgggta
      121 cggtcagcca gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg
      181 atcagggctg gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc
      241 aggtgcttgc tgttccgcca gcgtatacaa gccagcgttg cgcgtactgt ggtcatacag
      301 cgaaagagaa ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagcga
      361 acgccgatgt aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg
      421 gagagatggt gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga
      481 cagcctgaac gtagcaggga tccacgtcct tcagggcgtg gaggatgtca atatcggttt
      541 ctgtcagcga ttaaattcaa caccacgtaa caaacgcctc cggcaattaa tccccagaac
      601 gcagaaccaa tcccgaccag cgtcaatcca cttgccgtta ccagaaatgc caccaccgcc
      661 gcgtctcgct cacgctcatt atgcagcgcc tgatacaaac tgccgctaat ggtacttaac
      721 agcgccagac ctgccagcat ctggatccaa cttacgggca gggcagccat catcccggta
      781 atagcactac caaacagacc tgcgatcaaa taaaaaatgc ctgcaacggc agctgccagc
      841 caacgttgat ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc
      901 gcaataccga cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat
      961 acaattaatg gcgaaacagg agccgaatac ccggctgctt tcattgctgc gatacccggt
     1021 gcgttttgcg atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca
     1081 aacgaaaaat caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg
     1141 acaacgtcac cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca
     1201 tagcgcggcg caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta
     1261 aattgaccgt ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc
     1321 attgccgccg caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg
     1381 ccgcagagga ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt
     1441 agtccctgca atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca
     1501 ggtacgcgat accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc
     1561 gtcatccagc cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt
     1621 attgcccctg aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt
     1681 ggaggaatag aaaacagacg catagtcacc tcgtacgtta tagcgtacag gcaccgtagc
     1741 atttgtccgt tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa
     1801 aatctcgctc gctttttatc caccacactt aaacaactac gccagcagcg cggctggagt
     1861 ctttcgcgac tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt
     1921 aatgagtcca gcccgacggt cgcgacatta tggaaaattg ccaccgggtt gaatgtgcca
     1981 ttttcgacat ttatttctcc gccacagtcc gctacgcctt cagtttatga tccacaacag
     2041 caggcgatgg tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc
     2101 tcgattcaga tggcacccgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc
     2161 gaacatgtcg tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc
     2221 cttaattgtg gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt
     2281 ggggagcaaa ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa
     2341 ctatttcgca acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag
     2401 aatgtgaccg tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt
     2461 gcccgcgaag ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt
     2521 gcccgtcata atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat
     2581 cgttatggtg caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa
     2641 cccgcgcaac ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag
     2701 gatatgggga ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc
     2761 gacattcgta cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg
     2821 ctggcgcgag agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc
     2881 attgaattct ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct
     2941 catgcgcaaa cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca
     3001 tacacattga aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg
     3061 aaagataacg atcagacagc caacctcggc gcgctgattg atgctggcgt acgctccttc
     3121 aagattgaag ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc
     3181 cagatgctgg atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt
     3241 actgaacatt tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat
     3301 tttgtgaatg cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg
     3361 ccggtgggtg aagtattgaa agtggcgaaa gatcatctcg atgttgccgt caccgagcca
     3421 ctggcaaatg gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc
     3481 aatacggtcg agaaaaccag agaaaatcag taccgcgtct ggcccaatga aatgccagca
     3541 gatttgcaca aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag
     3601 caggcactga caaaaacctc cagcgaacgt cgggtggcgg tagacattga attgggcggc
     3661 tggcaggaac aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg
     3721 ctggacgggc agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat
     3781 ggtctggcaa aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg
     3841 ggggcgctgt ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg
     3901 ctggatgctg cgcgtcttgc cagttaccat cgcggcagcc gtaaaccggt tgctgatcct
     3961 gcgccggttt atccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg
     4021 cgtgaatttt atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa
     4081 gagaagggcg aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg
     4141 tgcccgaaac aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt
     4201 aacggcgatg aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt
     4261 ggcaaaatca aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta
     4321 agtccggatg agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg
     4381 gttactcaca actcattgaa tctgcatgat attgcctgcc gggtaaggtg tcacgccgca
     4441 tccggcaaca aatgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa
     4501 aatgctcgcg caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg
     4561 cttcatgacg cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc
     4621 ctgccatgag ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg
     4681 taaacatcct gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgagg
     4741 ttattattca gttttgcaaa ttagcgcaaa gaaattctgg aatcttcctt cctgattttg
     4801 catggcattc tgccgttgcg gcgatttagt gctattttcg agcaaattac acacggaggt
     4861 aaacactaat gtttactttg ttgatatact cagcggcagg gaggcgatgt gaaacaaagc
     4921 gagttcagac gttggctcga atctcagggc gtcgatgtag cgaatggcag caaccatttg
     4981 aaactcaggt ttcatgggag gcgcagtgtc atgccgcgtc acccctgcga tgagattaaa
     5041 gaaccattgc gtaaagcaat cctgaaacaa ctcggtttga gttaatcgcc aattaaaaag
     5101 gttaatgaca tgcgagggac agtcgaaatt atgcgttatc ccgtcactct tacacccgcg
     5161 ccggaaggcg gttatatggt ttcttttgtg gatatccctg aagcgttgac gcagggcgaa
     5221 actgtcgctg aagcgatgga agcggcaaaa gatgctttac tgaccgcatt tgatttttat
     5281 tttgaagata acgagcttat cccgttacct tcgccattaa atagtcatga tcactttatt
     5341 gaagtacctt tgagcgtcgc ctctaaagta ttgctgttaa atgctttttt acagtcagaa
     5401 atcactcagc aagagttagc caggcgaatt ggcaaaccta aacaggagat tactcgccta
     5461 tttaatttgc accatgcgac aaaaatcgac gccgtccagc ttgcggcaaa ggcgcttggc
     5521 aaagagttat cgctggtgat ggtttaatta cagttaacga aaagttgtca tttttaacaa
     5581 ctgatataga ctgccgaatc atctgcacat aattacgatt cgataatgaa aaaataccag
     5641 cagcttgcag aacaattacg cgagcagatt gctacgggta tctggcaacc cggcgatcgt
     5701 ttgccttcgt tgcgtgacca ggtggcgctt tcaggcatga gctttatgac tgtcagccat
     5761 gcctatcagt tgctcgaaag tcagggatat attatcgcac gaccgcagtc gggttattac
     5821 gttgcgccac aggcaataaa aatgccgaaa gagccagtca ttccagtcac tcgagatgaa
     5881 gcagtcgata tcaacactta tatttttgat atgttgcagg ccagtcgcga accgtcggtc
     5941 gttccgtttg cctcggcctt tcccgacccg cgacttttcc ccctccaaca actaaaccgc
     6001 tcgctggcgc aggtaagcaa aaccgccacg gcgatgagcg tgattgaaaa cttaccgcca
     6061 ggaaacgcag aactgcgtca ggctattgct cgtcgctatg ccttacaggg catcaccatt
     6121 tctcctgatg aaattgtcat tactgccggg gcgttagagg cattaaacct cagtttgcaa
     6181 gcggtaactg aaccgggcga ttgggtgata gtagagaatc cttgtttcta tggcgcgttg
     6241 caggcgctgg aacggctacg gctgaaagcg ttatcggtgg cgacggatgt taaagaaggg
     6301 atagatcttc aggcgttgga actggcgttg caggagtatc cggtgaaagc gtgctggctg
     6361 atgactaata gccagaatcc actcggattt accttaacgc cgcaaaaaaa agcacaactg
     6421 gtggcgttgc tcaatcagta caacgtaacg ctgattgaag atgacgttta cagcgaactt
     6481 tattttggac gggaaaaacc gctgcctgcg aaagcgtggg atcgccacga tggcgttttg
     6541 cattgctctt cgttttcgaa atgtctggtg cctggttttc gtattggttg ggtcgccgcc
     6601 ggaaaacatg cacgtaaaat tcaacgattg cagttgatga gtacgctttc caccagctca
     6661 ccgatgcagc ttgcgctggt ggattacctt tccacgcgcc gatacgacgc ccatcttcgt
     6721 cgcctgtgtc gccagcttgc ggaacgtaaa caacgtgcct ggcaggcact gctgcgttat
     6781 ctgcctgcgg aagtgaaaat tcatcataat gacagtggtt actttctctg gctggagctc
     6841 cccgagccgt tagatgccgg cgaattaagc ctggcggcac tgacgcatca tatcagtatt
     6901 gcgccgggta aaatgttttc taccggtgaa aactggtcac gttttttccg ttttaatacc
     6961 gcgtggcagt ggggagagcg tgaagaacag gcggtaaaac aattaggcaa acttattcaa
     7021 gaacggctgt aatagcgttt aatttaattc ctcttagatt gggtaatatg aatttcgaat
     7081 agcagtcata tttcctaact ccttgactat actccagaag ataaccttac agacggcata
     7141 atgcgcggta gctcacaacc tgattaaatt ttctcagggg cgaaggtgtg cctgcaagcc
     7201 gccgtctatg gttaaacaag gagatatttt tacggcacgg cggctgaaca attaattacg
     7261 acaggagtaa gaccttatga gcaagacatt tgcccgcagc agcctgtgtg cgctcagcat
     7321 gacaataatg accgctcacg ccgccgaacc gcctaccaat ttagataaac cggaagggcg
     7381 actggatatt atcgcctggc cgggatacat cgaacgcgga caaactgata aacaatacga
     7441 ctgggtaacg cagttcgaaa aagagacagg ctgcgcggtg aatgtgaaaa ccgccgcgac
     7501 ttccgatgaa atggtcagtc tgatgaccaa agggggttac gatctggtta cggcatccgg
     7561 cgatgcctcg ctgcgtttga ttatgggtaa acgcgtgcag ccgattaata ccgcattgat
     7621 tcccaactgg aaaacgctcg atccgcgcgt ggttaaaggc gactggttta atgttggcgg
     7681 caaagtttac ggcacacctt accaatgggg gccgaacctg ctgatgtaca acactaaaac
     7741 cttcccgacg ccgccggata gctggcaagt ggtttttgtt gagcaaaatc tgccggacgg
     7801 caagagcaat aaaggccgcg ttcaggctta tgatggccct atctacattg cggacgctgc
     7861 gttgttcgtt aaagccactc agccgcagtt gggcatcagc gatccgtatc aactcaccga
     7921 agaacagtac caggcggtgc tgaaagtgct gcgcgctcaa cacagtttga tccatcgcta
     7981 ctggcatgac gctaccgtgc aaatgagcga tttcaaaaac gagggtgtgg ttgcttccag
     8041 tgcctggccc tatcaggcca acgccctgaa agccgaaggc cagcctgttg ctaccgtttt
     8101 cccgaaggag ggtgttaccg gttgggctga taccaccatg ctgcatagcg aagcgaaaca
     8161 tccggtttgc gcctacaaat ggatgaactg gtcattaacg ccaaaagtgc agggcgatgt
     8221 ggcggcctgg tttggctcgt taccggtagt gccggaaggg tgtaaagcca gtccgttatt
     8281 aggcgaaaaa ggttgtgaaa ccaacggttt taactatttc gacaaaatcg ccttctggaa
     8341 aacgcctata gcagaagggg gcaagtttgt tccctacagt cgctggacgc aggattacat
     8401 tgccattatg ggcggtcgct aacttcgctg gggtgcttta tgacgtacgc agtggagttt
     8461 gacaacgtct cgcggttgta cggtgacgtg cgcgcagtag atggcgtcag tattgcgata
     8521 aaagatggtg agttcttctc tatgctgggg ccgtccggct ccggcaaaac cacctgcctg
     8581 cgcctgattg ctggcttcga acagctttcc ggcggggcta tctctatctt tggtaaaccc
     8641 gccagcaatc tgccaccgtg ggagcgggac gtgaatactg tctttcagga ctacgcgcta
     8701 tttccgcata tgtcgattct tgacaatgtc gcctatgggc tgatggtcaa aggcgtgaat
     8761 aaaaagcagc ggcacgcaat ggcgcaagag gcgctggaga aagtggcgtt ggggtttgta
     8821 catcaacgta aaccgtcaca actttctggt ggtcagcgcc agcgggttgc tatcgccaga
     8881 gcattggtga atgaaccgcg cgtattgctg ttggatgaac cgctcggcgc actggatctc
     8941 aaattgcgtg agcagatgca gctggaactg aaaaaactgc aacagtctct cggtatcact
     9001 tttatcttcg ttacccacga tcagggcgaa gcgttatcga tgtccgatcg tgtggcggtt
     9061 ttcaataatg gacgcattga gcaggtcgat tccccgcgcg atctctatat gcgcccgcgc
     9121 acgccgtttg ttgccggatt cgtcgggaca tccaacgttt ttgatccgct aatggccgag
     9181 aatctttgcg gcatgacggg aagcttcgcc ctgcgaccgg aacatatccg tctcaatatc
     9241 cccggcgaaa tgcaggctaa tggcacgatc caggcggtgc aatatcaggg cgcagcgacc
     9301 cgttttgaac tgacactgag cggcggtgaa aaactgctgg tgagtcaggc caatatgaca
     9361 ggcgaagaac tgcctgccac gctcacgccc ggacaacagg tgatggtttc ctggtcgcgt
     9421 gatgtgatgg tgccgctggt tgaggagagg tgaatggcga tgaatgtatt gcaatcacct
     9481 tcacgtccag gtctgggtaa ggtgtccggt ttcttctggc gtaatccggg gctggggctg
     9541 tttttactgc tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg
     9601 acactgttat ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa
     9661 ctgacgctgg cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc
     9721 acgctgacta tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg
     9781 tggtatatgg cgcgctatac cagcgggaaa atgaaagcgt ttttttatat tgcggtaatg
     9841 ttgccgatgt gggcgagcta cattgttaaa gcctatgcct ggacgttatt gctggcaaaa
     9901 gatggcgtgg ctcagtggtt tttacaacat cttgggctgg aaccactgct gactgcgttc
     9961 cttacattac ctgcggtggg cggaaatacg ctgtcaactt ccgggctggg gcgctttctg
    10021 gtgtttctct atatctggtt gccgttcatg atcctgcccg ttcaggcggc gcttgagcgt
    10081 ttgccgccgt cattgttgca ggcgtcggct gatctcggcg cacgtccacg acaaaccttt
    10141 cgctatgtgg tgctgccgct ggcaatcccg ggtattgccg ctggctctat ctttaccttc
    10201 tcactcacac tgggcgattt tatcgtcccg cagctggttg gtcctccagg atattttatc
    10261 ggcaatatgg tttattccca gcagggggcg attggcaata tgccgatggc ggcggcattc
    10321 accctggtgc cgattattct catcgcactg tacctggcgt tcgtgaaacg tctgggagcg
    10381 ttcgatgcac tctgaacgcg caccgttttt cctcaaactg gcggcctggg gcggcgttgt
    10441 tttcctacat tttcccatcc tgataatcgc cgcctatgcg tttaacactg aagatgcggc
    10501 gtttagtttt ccaccgcagg gcctgacgct gcgctggttt agcgtggcag cacagcgtag
    10561 tgatattctt gattccgtga cactgtcact taaagtggcg gcgctggcga cattaattgc
    10621 gctggtgtta gggacgctgg cagctgccgc gctgtggcga cgagactttt tcggcaaaaa
    10681 cgccatttcg ctgttactgc tgctgcccat tgcgctgccg ggcattgtca ctggtctggc
    10741 gttattaacc gcctttaaaa ccatcaatct ggagccggga tttttcacca tcgtggtagg
    10801 tcatgcgact ttttgtgtag ttgtggtgtt taacaatgtc atcgcccgtt ttcgccgcac
    10861 ctcgtggagt ctggttgagg cgtcaatgga tcttggggcc aatggctggc aaaccttccg
    10921 ctacgtagtg ttgccgaatc tcagttcggc gctactggca ggaggaatgc tggcgtttgc
    10981 cttgtcgttc gatgaaatca tcgttacgac ctttacggca ggtcatgaac gaacgttacc
    11041 gttgtggttg ctcaatcagc ttgggcgacc gcgtgatgta ccggtaacca acgtggtggc
    11101 actgctggtt atgttggtaa caaccttgcc gatcctgggg gcctggtggc taacccgcga
    11161 aggcgacaat ggtcaataac cactgataca ggaatatgct atgcaacata agttactgat
    11221 taacggagaa ctggttaccg gcgaagggga aaaacagcct gtttataatc cggcaacggg
    11281 ggacgtttta ctggagattg ccgaggcatc cgtagagcag gtggatgctg ctgtgcgcgc
    11341 ggcagatgca gcatttgccg aatgggggca aaccacgccg aaagcgcgtg cggaatgcct
    11401 gctgaaactg gcggatgtta tcgaagaaaa tggtcaggtt tttgccgaac tggagtcccg
    11461 taactgtggc aaaccgctgc atagtgcgtt caatgatgaa atcccggcgg ttgtcgatgt
    11521 ttttcgcttt ttcgcgggtg cggcgcgctg tctgaatggt ctggcggcag gtgaatatct
    11581 tgaaggtcat acttcgatga tccgtcgcga tccgttgggg gtcgtggctt cgatcgcacc
    11641 gtggaattat ccgctgatga tggcagcgtg gaaactggct ccggcgctgg cggcaggaaa
    11701 ttgcgtggtg cttaaaccat cagaaattac cccgctgacc gcgttgaagc tggcagagct
    11761 ggcgaaggat atcttcccgg caggcgtgat taacatactg tttggcagag gcaaaacggt
    11821 gggtgatccg ctgaccggtc atcccaaagt gcggatggtg tcgctgacgg gctctatcgc
    11881 caccggcgag cacatcatca gccataccgc gtcgtccatt aagcgtactc atatggaact
    11941 tggtggcaaa gcgccagtga ttgtttttga tgatgcggat attgaagcag tggtcgaagg
    12001 tgtacgtaca tttggctatt acaatgctgg acaggattgt actgcggctt gtcggatcta
    12061 cgcgcaaaaa ggcatttacg atacgctggt ggaaaaactg ggtgctgcgg tggcaacgtt
    12121 aaaatctggt gcgccggatg acgagtctac ggagcttgga cctttaagct cgctggcgca
    12181 tctcgaacgc gtcagcaagg cagtagaaga ggcgaaagcg acagggcaca tcaaagtgat
    12241 cactggcggt gaaaagcgca agggtaatgg ctattactat gcgccgacgc tgctggctgg
    12301 cgcattacag gacgatgcca tcgtgcaaaa agaggtattt ggtccagtag tgagtgttac
    12361 gctcttcgac aacgaagaac aggtggtgaa ctgggcgaat gacagccagt acggacttgc
    12421 atcttcggta tggacgaaag atgtgggcag ggcgcatcgc gtcagcgcac ggctgcaata
    12481 tggttgtacc tgggtcaata cccatttcat gctggtaagt gaaatgccgc acggtggaca
    12541 gaaactttct ggttacggca aggatatgtc actttatggg ctggaggatt acaccgtcgt
    12601 ccgccacgtc atggttaaac attaagttag atgttagtgg ctgttatagg acggccacta
    12661 tcgttttagg ttctgagttt aatggcggtt aagtattaga atgattacat taataactag
    12721 cgtaaaaatt aggctaaatg tttgatggcg atttttcatc ctgtcacagt tgattagcat
    12781 a
//
LOCUS       DAZGXZ010000138        12736 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000138 DAZGXZ010000000
VERSION     DAZGXZ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.138"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..244)
                     /locus_tag="ACHKJF_004237"
     CDS             complement(<1..244)
                     /locus_tag="ACHKJF_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0951495.1"
                     /translation="MDFNSGGKKAIERLVEAYGFGTRQALCDHLGVSKSTMATRYMRD
                     IFPADWVIQCALETGTSLNWLTTGHGSKQASANTNTI"
     gene            complement(430..1488)
                     /locus_tag="ACHKJF_004238"
     CDS             complement(430..1488)
                     /locus_tag="ACHKJF_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951496.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DSKQAALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKALELK
                     VYADDQMAKHLAAPDPHSQYAPKESPTFTGTPKAPTPAAGNNTTQIATTEFVQAALTA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVTGLLTYLRL"
     gene            complement(1485..2090)
                     /locus_tag="ACHKJF_004239"
     CDS             complement(1485..2090)
                     /locus_tag="ACHKJF_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP0951497.1"
                     /translation="MNSLLPPGSTSLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(2083..2991)
                     /locus_tag="ACHKJF_004240"
     CDS             complement(2083..2991)
                     /locus_tag="ACHKJF_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFP0951498.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAQFVALHPKDEQEAVI
                     RTLELESEPVTKLLQESAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVTRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(2978..3337)
                     /locus_tag="ACHKJF_004241"
     CDS             complement(2978..3337)
                     /locus_tag="ACHKJF_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP0951499.1"
                     /translation="MTLYSGMNNTSGKVITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSVLIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(3334..3912)
                     /locus_tag="ACHKJF_004242"
     CDS             complement(3334..3912)
                     /locus_tag="ACHKJF_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0951500.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRANWLCRVKTGDLETNWIN
                     WLTLRAGKSRTWWKPSVGEQVVLFSLGGNLETAFALPAVYSNQFPPPSGSEDGNVTEY
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITLKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            complement(3981..4427)
                     /locus_tag="ACHKJF_004243"
     CDS             complement(3981..4427)
                     /locus_tag="ACHKJF_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0951501.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFTKLRTTKYLKTAASADSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            complement(4420..4851)
                     /locus_tag="ACHKJF_004244"
     CDS             complement(4420..4851)
                     /locus_tag="ACHKJF_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951502.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDEPEEMWTVKRG"
     gene            complement(4814..5062)
                     /gene="lysC"
                     /locus_tag="ACHKJF_004245"
     CDS             complement(4814..5062)
                     /gene="lysC"
                     /locus_tag="ACHKJF_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461641.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFP0951503.1"
                     /translation="MLTLSGCGNVRRSPEVQLTVSGCPRVTPCWLDQAAPRTNGDLNA
                     LLDETEAAWAVCADKVDMIIACQERNSEQTTIPAPRPQ"
     gene            complement(4947..5375)
                     /gene="lysB"
                     /locus_tag="ACHKJF_004246"
     CDS             complement(4947..5375)
                     /gene="lysB"
                     /locus_tag="ACHKJF_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFP0951504.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSALLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVLAGSGSAAH"
     gene            complement(5372..5749)
                     /locus_tag="ACHKJF_004247"
     CDS             complement(5372..5749)
                     /locus_tag="ACHKJF_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFP0951505.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVAGLLT
                     WAVIPVALAALIKNGPLWQELRASLLKTITRKENVFISWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(5751..6263)
                     /locus_tag="ACHKJF_004248"
     CDS             complement(5751..6263)
                     /locus_tag="ACHKJF_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0951506.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDAAVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLQRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(6244..6459)
                     /locus_tag="ACHKJF_004249"
     CDS             complement(6244..6459)
                     /locus_tag="ACHKJF_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1 family phage holin"
                     /protein_id="HFP0951507.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(6463..6666)
                     /locus_tag="ACHKJF_004250"
     CDS             complement(6463..6666)
                     /locus_tag="ACHKJF_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP0951508.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(6666..7130)
                     /locus_tag="ACHKJF_004251"
     CDS             complement(6666..7130)
                     /locus_tag="ACHKJF_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0951509.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDSKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(7226..7876)
                     /locus_tag="ACHKJF_004252"
     CDS             complement(7226..7876)
                     /locus_tag="ACHKJF_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFP0951510.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(7880..8938)
                     /locus_tag="ACHKJF_004253"
     CDS             complement(7880..8938)
                     /locus_tag="ACHKJF_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP0951511.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRDAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSSTTGGETTPRAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            complement(8955..9788)
                     /locus_tag="ACHKJF_004254"
                     /pseudo
     CDS             complement(8955..9788)
                     /locus_tag="ACHKJF_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
     gene            9931..11697
                     /locus_tag="ACHKJF_004255"
     CDS             9931..11697
                     /locus_tag="ACHKJF_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFP0951512.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            11697..12722
                     /locus_tag="ACHKJF_004256"
     CDS             11697..12722
                     /locus_tag="ACHKJF_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0951513.1"
                     /translation="MGKSKKNRAAATNQLKHKSQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQMDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLLEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLVFVRNELIPLQKRMQELNKWLEDNIL
                     QFKPYKF"
BASE COUNT         2765 a         3741 c         3323 g         2907 t
ORIGIN      
        1 ctatagtatt tgtatttgct gatgcttgct ttgaaccatg ccctgttgtt agccaattaa
       61 gcgaggtgcc cgtttcaagg gcgcactgga ttacccaatc tgctggaaaa atatcacgca
      121 tatagcgcgt tgccatggtg ctcttagaaa cacctaaatg atcacagaga gcctgacggg
      181 taccgaaccc atatgcttca actaaacgtt ctatggcttt cttaccgccg ctattgaaat
      241 ccacaagtcc tccaaagaaa tccaaaattc gttgacagat tccaaaagcg atcttaaagt
      301 tgagccagaa gtgttctttt ggagccttca ctactaatca cgacaaacaa cggctcgcca
      361 caagccatat ctagaaggaa tgttgcctta tgacacctaa catttcaatt actctgaata
      421 caccacatgt cacaatcgaa ggtatgtgag aagaccagtt acatcctttc cactcaaatt
      481 agtcagcgta ttgtccagcg gttgtttacc tgccagtgca ttaagcattg tcgtggcaaa
      541 gttcgggtca ttccccagcg ccgccgccag ttcgttcagt gtatccagtg ccgcaggtgc
      601 agaacccacc attgccgcaa tcgccgattt cacaaatgcc gtggtggcaa tttgtgtatt
      661 gttgaccgac tgtgccgcag taggtgcggt tggcgttccg gtgagtgccg gactcgacag
      721 cggcgctttc agtgccagcg cattgttaat ggtggtactg aatttcggat cattgttaat
      781 ggctgcggct atttctttca gcgtgtccag cgtggctggc gcaccattaa taagagccgt
      841 cagagccgcc tgaacaaact cggtggtcgc aatctgcgtg gtgttattcc ctgctgctgg
      901 cgttggcgct ttgggtgtcc cggtaaacgt cggactttct ttgggtgcat actgtgaatg
      961 cgggtccggt gcggcaagat gttttgccat ctgatcatcc gcgtacacct tcagctccag
     1021 tgctttgtca tccacatact tgcgggttgc cagcactaca gcagggtcga ttttcagggt
     1081 gatattgtcc gtgctgctgg taatcagcac catgcgcacg gtctgagtgc gcccgctacc
     1141 ttcagccagt tgcggcttat agctttccgg gcagttgccc acggcaatca atgccccgga
     1201 ctcatcaaac aagcccactt cacgtatcca ccaaccgccc tcgttttcag ggatcacctg
     1261 ttcggcaata atctgactgc tgttctgcgg gtcgatatag agcatattca gcgcagcccg
     1321 gcgtttctca tttaccagtg cagcctgctt tgagtctggc gttggcaata ctccgccgcc
     1381 atcgccgacc gccatatggg taatttttag cggcacaccg agcgcggcgg cgctggcaag
     1441 tttcgccgcg ccaatatccg tcagcagggt ataaaatttt gtgctcatgg attcactctc
     1501 attgtgtcaa taacatggac cgccccgcct tcatgcgcgg tgccgccaga aataattgtt
     1561 tcgttgatat acggatagat cgtgatttct tcgccaagat agctggcggc ccccacccaa
     1621 tgcggaccgc tggtctgcag attgatggac atgccgatca tgtgacggct acatggtttg
     1681 gcatcgctta tcagccgctc aagttccaga taggtatctt cagtgatgcc ctggtcctgc
     1741 acgccgatat ccaggcgaaa cgtgcccggt gtttctccgg tctgccacca ctcaataatg
     1801 cggatcagga atccgaacgg ttccaccacc cgccgcacgg cactggtggt ccctttatgc
     1861 tgatgaatat aaaaagcatc cttcactacc tggcgtttga cgctttctgt ccagccctcg
     1921 tcccagcgat ccacagagaa cgcccaggcg agataaggca ggaagctgac cggacaggta
     1981 gccggattcc acaagtcacg cagcggcacc tgcagatcag aaatcccgct acaggtttgc
     2041 gccagtcggc gctccagtga agttgaaccc ggtggcagca gactattcat ccgttcctcc
     2101 gttggttacg ctccactgcg tacatgatgc cgcctgtgtt ttgttcagga ccacatccgc
     2161 cagcggagaa gccagttcca cacgttgaac accctcaaca tgcagagcag caaagatggc
     2221 gctacggcga atatcccgac caagcctcgt ctggctggca atgtacttct gcagactggc
     2281 ttttgccgct gccattaccg gctctgcttc cggtcccgga tagagaaaaa tggtggcttc
     2341 cacgcgatac gggatgattt ctgcgctgcg aaccgtaaga cggtcagcca ccgggcggac
     2401 gttctcactg ttcagagctt tttccaccac gtccagcagg tctttttctg cagttccatc
     2461 gccttcgcgg ctaaggacag tcagcaccac ctctgcaggt gccgggctgg ttgcactggc
     2521 atccgctacc cgaccgtcgg cgcttcgggc atgaaattca taagctgcag ttggccccgc
     2581 aacagaaagc ccttcaaagg ctgcaggcac acgcaggcgt aacgcttcat cgctttccat
     2641 cacagctgca acgggcggca cagcatcatt atcagcaggc gtcaccgtca ggcgtgtcac
     2701 gttgtagttg gcagcgagct ggtcaagatc gccgcccatc gcgtaagcca ccatcaccgc
     2761 ctgcgcggct tcgttaatgc gctggcgcag aagcaactca cggtaagcgc tctcctgcaa
     2821 caatttagtg acgggttcag attccagttc cagcgtgcgg atcacggctt cctgctcatc
     2881 tttcggatga agcgcaacaa attgggcctt gcgttcggca agcagcgtct caaagtccgg
     2941 cacatccaca atctgcggtg caggcaactg cgaaaggtca atcactgcca ttctctgctc
     3001 ctgttgatac ggaaagggac acaggcacac cgttattccg ccgcccggtc agctccacca
     3061 ccatagaacc gtcaaagttg ctgttgatgg tgatggaatc cagcgtcagc cgtggctccc
     3121 agcgactcag cgccacatac actgccgaca tgacctgcag gcgtaatgcc ggattttgtg
     3181 gctgatctat taaaaccgac aggagggaac catattctcg gcgggcaatg cggctaccct
     3241 gcggtgtcag cagaatgtcc cgcaccgact ggcgcagatg atcaatatca gtaatgactt
     3301 tgccgctggt attgttcatc ccgctataaa gcgtcatacc gggcctccgg ttgtatcgcc
     3361 gcctttcagg acgccagtat gctgatgcgc atcaaccaca atcccgttag agctcatcgc
     3421 tccgccgccc tgggtaacgc caccattgat caccacttcg ctgttaatac gcgtgcggtc
     3481 agcctccagc acaaactcac tggttttcag ggtgatattg tcagcggcct caatgaccat
     3541 tgatttgatg cccctgacat accagcgccc ggtggcgggt tcgtattcaa accagccgcc
     3601 gtccgggtat tccgtcacgt tgccgtcctc agagcctgaa ggtggcggaa actggtttga
     3661 gtagaccgca ggcagggcaa acgcggtttc cagattgccg ccaaggctga acagcacaac
     3721 ctgctcaccc acagacggtt tccaccaggt gcgcgatttg cccgcgcgca gtgtcagcca
     3781 gttaatccag ttggtttcga ggtcgccagt tttcacccga cacagccagt ttgccctgtc
     3841 cacttcggtg actacacctg tgcggatcag gttggtgata aggcgcatga tttcggttaa
     3901 ttgtgcgttc atagggaaag gttgccatca ggggaagaaa ggcggcagtg ctgcaacttg
     3961 tatcagtgct gatacaaaga tcaccccgcc agccattgca gaatcatgtc gcgggtcatt
     4021 gcctcaacat catcatttac acccagaagg cgacgctctg cgtaacggac ctcaggtcct
     4081 ttgcgactga cacgatcgcg caagccgtaa tggtgaacac gggcaatgcg ctgcacctta
     4141 ccttcaaact gcacgctggc agagtcggcg ctggcggcag ttttcaggta ttttgtggtg
     4201 cgaagttttg taaacatctg ccgtttgata cggccttttt tactgcgtgc tgttactcgc
     4261 ctcggttcat agctgctgcc atcaggattg cgttgcatcc tgatgttctg ctgctgtgtc
     4321 cggcgcagtt cctgcgccag ctggcgcatc atgcggcttc tggcggctgg ttccagattc
     4381 gccagcaagg cactcagcca gtcgtccact ttctgcagtt cagccacgtt tcaccgtcca
     4441 catttcttca ggttcatcag gttccgctat agcttcaaca ctcgacacac taccgtcagt
     4501 gctgaccagc acacgctccg tcagttgcag gttcaggctg atatcacaga catcgttgcg
     4561 cagaatatcc acctcaaagg tgaatagttt ttcccgtaac gccgggttat tgatggcatc
     4621 gggctggtta tccctcagcc acagcaaaac cggggccatc agcagattct ggtcgccgct
     4681 gaaatcctca atcaccgcgt tcagggtgta acggtactcc cacgacatgg agctggcccc
     4741 cgtggcaacc agcgaaccgt tatccacaaa cagatgcagt ttgtccgggt tattgcggac
     4801 ataaggcacc gctttattga gggcgtggcg cagggattgt ggtttgttca ctgtttcgct
     4861 cctgacacgc aataatcatg tccactttgt ctgcacagac cgcccaggcg gcctccgttt
     4921 catccagcaa cgcgttcaga tcaccgttag tgcgcggcgc tgcctgatcc agccaacacg
     4981 gcgtcactcg cggacaacca ctgacggtaa gctgcacctc cggtgagcgt cggacgttcc
     5041 cgcagccgga taatgtcagc aggcaaagga gtatcagccc agcggcgtaa atcctcgttc
     5101 tcacgtttca gttcctcgat ccggtgttgt cgttgtctca gcagcgcgct ggtctgttct
     5161 gcttcggcat agagccgcgc ctgctcccgg ttattggttt cagtcagaat ggacaggctg
     5221 ataagctggc tgttgctctt tgccagtgcc tggcttttgc tctgcagctc gtctgcctgc
     5281 gtgctaatgg tctggctggc atcagccagc cgccacgtct gccagcccag cgccgccagt
     5341 aataacgcca gcacaaccag cagcaaccgg ttcatgctgc tacctgttgc gccatctggt
     5401 tacgggtgat ccagaaggca ataacggtca gtagataaaa gaccagggta atagcccacc
     5461 ccgtccaggc gagacttaca acaatcagca atcgcatcac ccaactgata aatacgtttt
     5521 cttttcgggt aattgtcttc agtaaagatg cccttaactc ctgccagagc gggccattct
     5581 taattaacgc agccagtgct accggaatta ccgcccatgt cagcaaaccg gctacccaaa
     5641 cgccggacgc tgccagtacc ggaaaaatcc cctgcggata caccattgct gcgattaaca
     5701 gcgccatcca taacatcaga aacagtccgc tgattaattt ctttttcatt tcagtttgct
     5761 ccctgtaaac accaggccat ctcccgcgca cggcggttat ccagcccctg attaaacaca
     5821 ccttttacat aaacccagcg ctgcaactgt cggcacgcat ccgcccagcg ccgctgattg
     5881 agcaatttca ccagcgtgga gctgcaggca ttgcccgtcc ccacgttgaa ggcaaacgac
     5941 accgcagcgt catacacctt ctgcggtggc tgttgcttca cacacctttc cagtgcccgc
     6001 tccacacgca gcacgttgga gatcagccct tctgctgcct gtcgttccgt gattgttttg
     6061 ccgggaataa cgcccgatgt attaccaatg ccgtcggtcc agacacccgc gctgcactga
     6121 tacggctgca gacgacagcc ttcgtaatca gcaatcagtt tcagcccctc cacggaggtg
     6181 tgaagctgct gaaaacccgg cagcgtggca gcaatagcca gcacggcccc gacaaggcag
     6241 cgtttaacga ttgatggatt catagtcctc ccgcgagatc tgcccgtcgc gcagaagctg
     6301 gtaggctttg tgtttgtagt accagttgat agccagcatc agcacaccaa tcatcaggcc
     6361 gcccagcgtt gaggcatcct tgatggacaa atcgcccagc caggccagca cgacggcgat
     6421 gcaatacgtg ataaaggcgc tgattcgctc aagcgtcata attcagtccc atagctggac
     6481 ggtctgcacg gtggtggtgg tcggaatgtc cggcagctcc acctgcagcc cgtgaggtaa
     6541 aaaggggccg tattcggcaa gccccggatt tgccttcagt acctgctccg tgacaccctg
     6601 cgtgcgcccg taatgacgcc agcaaagcgc gtccaccgtg tcatactgat gcgcacgcac
     6661 tttcatcaga taagctccac tgtgcagtgc ggcgcatcct gcacccggct gatggcccag
     6721 cgggcgtcac gccacaaatc accgcttgct tccgccagtt cctcgccccg cttcacaccg
     6781 gacgccgtgg cgtcatagtc ctggtaacgt tcgttgagca tggcgcgtgc ccagcagtaa
     6841 accgcgttga aatagtgctg aatgcgctca cttttgctgt ccagctgttc cgccggaacc
     6901 tctgccagcg aggcataccc cagcatctgc tggcgtctgc gaaactcata cagctctgcg
     6961 ttgacctccg aaattgccga cagcgcaacc tgctttaaac gcggctgcgt caccgtgccg
     7021 tcagtgcgca tcacgctgcg aaactccgac aggtccacat caggccagaa cggcgtattc
     7081 ctgatgattt ccgcctgttc cggtgcctgt tctggcgcaa caaacttcat gctgctttct
     7141 cctgaaatag agggcggtgg acggggtttt gatgtggcag tgcctttcgc caccccgtgc
     7201 cgcccgtgtg cgggggcacg ttctgtcagc ggctgtcatt gcgcagtctg cgctccagct
     7261 gctgtttgtc ttttttcacg ccacagcgag gatcgagctg taacgcatgg ttgagatgat
     7321 taagggcgga agccgggttg ctttcactca ggacagcgcc aatcgcttta tgcagacgcg
     7381 cccgtgactg gtccggcata tccagaccgt ctgtcagctc cagcgtctgc agcaacaggt
     7441 cggcatcaaa gccggtggcg gcaagcattg cgctctgggc tgcatctgcc atttcctctg
     7501 ctagcacggt ctgcacgttg cggttaccca gcggcatcac ccagccatga cgcagggcat
     7561 gacgcccgat ctccagcgcc ccggcataat ctccggcatc aatgcgccac agcatcacgt
     7621 acatcagcac gtcatcctgt tgagcgcctc cggcagccag aacgccctcc gcccaggcgg
     7681 catatttcgg cagcagctcc accttgattt ccgctttttt gaccgtggac tgaacgccct
     7741 tgagacggcg gcggtcttcc gccagttgca gcagcatcag gtcatagccc gacgcgtggc
     7801 gaacactacc accctcgcga gcggcctgtt cagcctgaac gcgcaggcga tgctgccgtg
     7861 cgggactcag gctcatgaat tacgctccgg tttctgctgc ggcggcgctg aagtcaccaa
     7921 tctggatgtt ttccaccagt gcagcgcagc gatagtcctc aaccacatag gcttcgttaa
     7981 cggattcaaa attttcaatc cggtcacgtt tcgggttgtc gataactgaa cggcggcggg
     8041 tatcttcctg ccagtagatg gacaggttat ccagacgggt gatcaacagc gcattcggcg
     8101 ggaagaacgg cgcacgcacc gcctgcaggc cacccatgcg tttctgactg atgatcatat
     8161 cggcagccag tttttcactg ttttcctgct ctttgttgac cagcgggaaa tacttgtcag
     8221 acagcagttc acgcccgcaa atcaccacca gatcgtcatc gtcctggtag acaacgtcga
     8281 taagctcatt gacggcatcc atcaccacgg cgtccaggtt ggcatattcg ccacctttac
     8341 cgactttcac cgcacgcggt gtggtttcac cgcccgtggt gctgctgccc atgacgtgat
     8401 ccggtgcatc ctcacggatt ttctgtaacc agcctttgtt cacatcctgc agcagcgggt
     8461 tttcgctacg gttggaggtt ttcgcacgct tcacgccgtt aaagccgatc atgatgcggt
     8521 ccagtgcctg acgtttcacg atggcgtcac ggatacgcac ctggaaatcc tgaaacttcg
     8581 cccacaggtc cagcttcgcg taggtcagta ccgtgtcaaa gttggtctgc tcgcatttgt
     8641 attccacatc gaccatcagc gtaggatcga caggttcacg ctctttcgcg gtggtgtcag
     8701 tggttccggc aatggtgctg ccaacaccca accccagcag ctgaccggac tgctcagtca
     8761 ctggcgtgac gttaatcagc gtcaggaaag cggcggactg ctggatctgg tcttccagcg
     8821 tctgctgtac agacggctcc acggtgaact tgctggacag ttcttcaact gccacaccgt
     8881 tcagacgcgc cagctgctgc aggtaagcgt taaaagcaaa tcgggtattc ttcttcatcg
     8941 ggttttgtgc tccatcagca attggtcaga gtgtcagcgg gggcgttacc gcctgttgca
     9001 cgctggcggt agtcctggcg gcagtcttca tgactcagct tgtccaccag ttcgttaaag
     9061 gcggtttgct gtgcctgcag ggcagtctcc agctcagaca ggcgttcttc ctgctcagac
     9121 agggattttt cggtgcgtgc gctcaggttc tgctgctcag tggcgaccag ctccacggcc
     9181 taatgcacat cagagaaccg ggcgtcatcg gactgctctt ttttggtaaa cagcgccgtg
     9241 acacgggcaa acagggacgg tttgtcgtcc tggatttctt ccagttcgat caccgtttcc
     9301 tctgcagcgg taaagagatt ggcaggattc tgcttgcggt ttgccagtgg gttatgggct
     9361 gcactggcgc tgaatgtcag catttcagtg cccagactgg cagggtcatc agtggctgcc
     9421 aggccgacca ggtaggcttt gcccgtatca gcgaacttcg ggctgacttc catagaggtg
     9481 aataatttct ggcctttttt caccagctcc accagggact ccgttggctc aacgtcggca
     9541 tacagcgcca tcttgcctgc cagcggacct tccatgattt cttcagcaaa cagcgccgtc
     9601 accttgccgt agcggttaaa ggtgctgtcc ggcagataag acttgatgtg ctcaaggtta
     9661 atcagcgcgg tatacaccgc cgggttgtag ctggctgcca tctgttccag ccattcacgc
     9721 tggatttcgc gtccgtcggt ggtggcacct tccaccccga tgcgaaaacg ctttgctttc
     9781 actgtcatga gccgtgctcc gttagaaaaa acttactgga gccttatggt tgcggtgatg
     9841 ggggcagtga aacaatgcgc ggtatttgta ccgacaacca cacaaaccgc aggcggggaa
     9901 agccgtcatt caaggctgta ggtttgtgcc atgaacacca cactgacacc cgcagatctc
     9961 gatccccgtc ggcaggccat gctgctgtac tttcagggat accgcgtagc ccgcattgct
    10021 gaaatgctgg gcgagaaagt tgcaaccgtt cacagctgga aaaaacgcga caagtggggt
    10081 gactatgggc cgctggatca gatgcagctc accaccgccg cacgctactg ccagctcatt
    10141 atgaaggagc acaaagaagg gaaagatttc aaagagattg acctgctggc gcgccagtct
    10201 gagcgccacg cgcggatcgg caagtttaac aatggcggca acgaagccga cttaaaccct
    10261 aacgtcgcca accgcaataa aggcccacgc cgtcagccgg aaaagaatgt tttcaccgat
    10321 gagcagattg agaagctgga agaaatcttc cattcctcca tgttcaacta ccagcgccac
    10381 tggtgggaag ccggaaaaac caaccgcatc cgcaacctgc tgaagtcacg ccagatcggc
    10441 gcgaccttct attttgcccg tgaagccctg attgacgccc tgcttaccgg acgtaaccag
    10501 attttccttt ctgccagtaa ggctcaggcc cacgtcttta aacaatacat catcgacttc
    10561 gccaaagaag tcgaggtgga gctaaaaggc gatccgatgg tgcttcctaa cggagccacg
    10621 ctttacttcc tcggcaccaa tgcccgcacg gcccagagtt atcacggcaa cctgtatctg
    10681 gatgaatatt tctggatacc gaaattccag gagctgcgca aagtggcttc cgggatggct
    10741 attcacaaaa aatggcgaca aacctatttt tccacgccat ccagcctgac ccacagtgct
    10801 tatccgttct ggtccggtgc gctgttcaac cgtggacgca acaaagctga caaggtggac
    10861 atcgacctgt cccacagcaa tctggccccc ggcctgctgt gcgcagacgg gcaataccgc
    10921 cagatagtca ccgtggaaga tgcggtgcgc ggcggctgta acctgttcga cctcgaccag
    10981 ctacgcatgg agtacagccc ggacgaatac cagaacctgc tgatgtgcga gttcgtggac
    11041 gatctcgcgt ccgtgttccc gctcagcgaa ctgcaggcgt gcatggtgga cagctgggaa
    11101 gtctggaccg actttcatgc actggccctg cgcccgtttg gctggcgcga agtgtggatc
    11161 ggttatgacc cggcaaaagg tacgcagaac ggcgacagtg ccggatgcgt ggtggtggct
    11221 ccgccagccg tgccgggtgg taagttccgc attcttgagc gtcaccagtg gcgcgggatg
    11281 gacttccgcg cccaggctga cgccatcaaa aaactcaccg aacagtacaa cgtgacctat
    11341 atcggtatcg actcaaccgg cgttggtcac ggggtttatg agaacgtgaa agcgttcttt
    11401 cctgccgtcc gggagtttgt ctacaacccc aatgttaaaa acgccctggt actcaaggcc
    11461 tacgacatta tcagccaccg ccgtctggag tttgacgccg ggcacaccga cattgcgcag
    11521 tcattcatgg caatccgtcg cgccaccacc gccagcggca accgcccgac ctatgaagcc
    11581 agccgcagcg aagaagccag ccacgccgat ctggcctggg caacgatgca cgcactgttt
    11641 aacgaaccgc tgcagggcga atccgccaat accagcaata ttgtggagat tttttgatgg
    11701 gaaagagtaa gaaaaaccgc gctgcggcga cgaatcagct caagcataaa agccagactt
    11761 cagccgaagc attcagcttt ggcgatcccg ttcctgttct ggaccgccgt gaactgctgg
    11821 actatgtgga atgcgtacag atggaccgct ggtatgagcc gcccgtcagc tttgacggac
    11881 tggcacgaac cttccgcgct gccgtgcatc acagctcacc gattgcagta aaatgcaaca
    11941 ttctgaccag tacctacatc cctcacccgc tgctcagcca gcaggctttt tcgcgttttg
    12001 tacaggacta tctggttttt ggtaacgcct acctggaaaa acgcacgaac cgcttcggag
    12061 aggtcatcgc tcttgagcct gcgctggcaa aatacacccg acgcgggtta gacctggata
    12121 cctactggtt tgtgcaatac ggtatgacaa cccagccgta tcagttcacg aaaggcagca
    12181 tctttcatct gttggaaccg gacatcaacc aggagatcta cggcctgccc ggttatcttt
    12241 ctgccattcc gtcagctctg ctcaacgagt ccgccacgct gttccgccgc aagtattaca
    12301 ttaacggcag tcatgcaggc ttcatcatgt acatgaccga tgctgcgcag aaccaggagg
    12361 atgtgaacaa cctccgcaat gcgatgaaaa gcgccaaagg accaggtaac ttccgcaatc
    12421 tgtttatgta ctcgcctaac ggcaaaaagg acggacttca gatcatccca ttgtcagaag
    12481 tcgcggcgaa ggatgagttt ctgaatatca aaaatgtcag ccgcgatgac atgatggcag
    12541 cgcatcgtgt gccaccgcaa atgatgggga ttatgccgaa taatgttggg gggtttgggg
    12601 atgtggaaaa agcgagttta gtgtttgtga ggaacgaatt aataccctta cagaaaagga
    12661 tgcaggaatt aaataaatgg cttgaagata atatattaca attcaaacca tataaatttt
    12721 aacgaaaaat cagcct
//
LOCUS       DAZGXZ010000139        12379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000139 DAZGXZ010000000
VERSION     DAZGXZ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.139"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..161
                     /locus_tag="ACHKJF_004257"
     CDS             <1..161
                     /locus_tag="ACHKJF_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0951514.1"
                     /translation="FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEP
                     TEMIQATA"
     gene            205..312
                     /locus_tag="ACHKJF_004258"
                     /pseudo
     CDS             205..312
                     /locus_tag="ACHKJF_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            complement(296..934)
                     /gene="ytfB"
                     /locus_tag="ACHKJF_004259"
     CDS             complement(296..934)
                     /gene="ytfB"
                     /locus_tag="ACHKJF_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP0951515.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEKQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1153..1773
                     /gene="fklB"
                     /locus_tag="ACHKJF_004260"
     CDS             1153..1773
                     /gene="fklB"
                     /locus_tag="ACHKJF_004260"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0951516.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGVSIPPFSTLVFEVELLEIL"
     gene            2082..3494
                     /gene="cycA"
                     /locus_tag="ACHKJF_004261"
                     /gene_synonym="dagA"
     CDS             2082..3494
                     /gene="cycA"
                     /locus_tag="ACHKJF_004261"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP0951517.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3539..4201)
                     /gene="ytfE"
                     /locus_tag="ACHKJF_004262"
     CDS             complement(3539..4201)
                     /gene="ytfE"
                     /locus_tag="ACHKJF_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP0951518.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4309..5274)
                     /gene="ytfF"
                     /locus_tag="ACHKJF_004263"
     CDS             complement(4309..5274)
                     /gene="ytfF"
                     /locus_tag="ACHKJF_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0951519.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5382..6242)
                     /gene="qorB"
                     /locus_tag="ACHKJF_004264"
     CDS             complement(5382..6242)
                     /gene="qorB"
                     /locus_tag="ACHKJF_004264"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0951520.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKTAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEVGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGHPTTTLAE
                     SVSHLFNVNN"
     gene            6331..6711
                     /gene="ytfH"
                     /locus_tag="ACHKJF_004265"
     CDS             6331..6711
                     /gene="ytfH"
                     /locus_tag="ACHKJF_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP0951521.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGGLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6829..8772)
                     /gene="cpdB"
                     /locus_tag="ACHKJF_004266"
     CDS             complement(6829..8772)
                     /gene="cpdB"
                     /locus_tag="ACHKJF_004266"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP0951522.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8962..9702
                     /gene="cysQ"
                     /locus_tag="ACHKJF_004267"
     CDS             8962..9702
                     /gene="cysQ"
                     /locus_tag="ACHKJF_004267"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP0951523.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEARQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9692..10250)
                     /gene="ytfJ"
                     /locus_tag="ACHKJF_004268"
                     /pseudo
     CDS             complement(9692..10250)
                     /gene="ytfJ"
                     /locus_tag="ACHKJF_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
     gene            10574..10780
                     /gene="ytfK"
                     /locus_tag="ACHKJF_004269"
     CDS             10574..10780
                     /gene="ytfK"
                     /locus_tag="ACHKJF_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP0951524.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10842..12185)
                     /gene="paeA"
                     /locus_tag="ACHKJF_004270"
     CDS             complement(10842..12185)
                     /gene="paeA"
                     /locus_tag="ACHKJF_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP0951525.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
BASE COUNT         2901 a         3117 c         3221 g         3140 t
ORIGIN      
        1 aattcagatg ccaggtatgt ggatatacag cgaacgccga tgtaaacggc gctcgtaaca
       61 ttttagcggc ggggcacgcc gttcttgcct gtggagagat ggtgcagtca ggccgctcgt
      121 tgaagcagga acccaccgaa atgattcagg cgacagcctg aacgaagcag ggatccacgt
      181 ccttcagggc gtggaggatg tcaacatatc agtatggttt cctcccaggc actggaagcc
      241 agacgtaagg tcatcaaaga gacaatcctt agctggcttt tggttgaccc ttcatctacc
      301 gcgcacgaat aaaactgcca tccggctggc gggtaaacag gacctgttga ttattccccg
      361 tatcaatggt taagcccgtc accacgccgc tggcgttctg gcggattttc accatttgcc
      421 cgttttgcaa attactcagc ggcttacccg cgccttctac ctgcgccatt gcatagacat
      481 cggtagcggg cagtccgtgg tcgcggaaca gctgcgccat cgttttaccg ggttcgacac
      541 ggtaagagcg ccattgatta tcaattccgc tatctggctg gaatggttgc gtttgggtag
      601 tttgcggctg cttttctggc tggccttcct gtataggttc tggagcgacc ggagccacct
      661 gatcaggatc attttgtggg gtgactaatt gcgcccgcag ctgttcttct gtcggcggtt
      721 gtgattgcga ctgaatatct aactgcgctt cacgcgtcac caccggcgcg ttgggcgtat
      781 catcagatgg aagcagaaat ccgaccacca atacaatggc ggcaataatg atcccccgac
      841 gatgcatcgg cggcagcggg tccatgaagc gaaaattgtc cggcgcgtgc cagactttct
      901 ccagggttgg ttttagttca aagcgcccgg gcatggtttc ctcctgctcc gcgtcttgtt
      961 cctcaatcat agcctatgaa taagctaacg ctatgatgtc cgtggtaaac ccgcctttat
     1021 tatattcata cgggatattg ctattgtttc tttttccccg ggatttgtca tcattcctgc
     1081 gacaaagttt tacccaaaga agtgtggctg atatgctgcc cgctactttt ataccctaag
     1141 aaaggaaata cgatgaccac cccaactttt gacaccatcg aagcgcaagc aagctacggc
     1201 attggtttgc aggtagggca acaactgagt gaatctggcc tggaagggct gctgccagaa
     1261 gcactggttg caggtattgc cgatgcgctg gaaggcaaac atccggctgt tccggttgat
     1321 gtggtgcatc gcgcgctgcg tgaaatccac gaacgcgccg atgccgttcg tcgtcagcgt
     1381 ttccaggcga tggctgctga aggtgtgaaa tacctggaag aaaacgccaa aaaagaaggt
     1441 gtgaatagca ccgaatctgg cctgcaattc cgcgtgatca accagggtga aggcgcaatt
     1501 ccggcacgta ccgaccgcgt tcgtgttcat tacaccggta aactgatcga cggcaccgtg
     1561 tttgacagct ccgttgctcg tggtgaaccc gctgaatttc cggttaatgg cgtgatccct
     1621 ggctggattg aagcactgac tctgatgccg gtaggttcta aatgggaact gactatcccg
     1681 caggaactgg catatggcga gcgcggcgca ggcgtatcca tccctccgtt cagcaccctg
     1741 gtgtttgaag tcgaactgct ggaaatcctc taagcagcgc attctgttcc cctcgaacca
     1801 gaggggagca ggcattcagc aataaaccct tcagtttgcc aaacggcgct attttgtgtt
     1861 gcaaagaccc cgtaagcgtg tatttttgtg agctgtttcg cgttatcacc gtgatatgac
     1921 actcacttta aacataaaat taacattaga tctaaatctt agtattcatc ccgcgtattg
     1981 ttacctaata tcgatgagtc ccgatacaga ttcgtcgtat catagactga ctaaaggccg
     2041 tagagcctga acaacacaga caggtacagg aagaaaaaaa catggtagat caggtaaaag
     2101 tcgttgccga tgatcaggct ccggctgaac agtcgctacg gcgcaatctc acaaaccgac
     2161 atattcagct tattgccatt ggcggtgcca ttggtacggg gttgtttatg gggtctggca
     2221 aaacgattag ccttgccggg ccgtcgatca ttttcgttta tatgatcatc ggttttatgc
     2281 tctttttcgt gatgcgggca atgggggaat tgctgctttc gaatctggaa tacaaatctt
     2341 ttagtgactt cgcttccgat ttactcgggc cgtgggcagg atatttcacc ggctggactt
     2401 actggttctg ctgggttgta accggtatgg cagacgtggt ggcgatcact gcttatgctc
     2461 agttctggtt ccccgatctc tccgactggg tcgcctcgct ggcggtgata gtgctgctgc
     2521 tgacgctcaa tctcgccacc gtgaaaatgt tcggtgagat ggagttctgg tttgcgatga
     2581 tcaaaatcgt cgccattgtg tcgctgattg tcgtcggcct ggttatggtg gcgatgcact
     2641 ttcagtcacc gactggtgtg gaagcgtcat tcgcgcattt gtggaatgat ggcggctggt
     2701 tcccgaaagg tttaagtggc ttctttgccg gattccagat agcggttttc gctttcgtag
     2761 ggattgagct ggtaggtaca accgctgcgg aaaccaaaga tccggagaaa tcactgccgc
     2821 gtgcaattaa ctctattccg atccgtatca ttatgttcta cgtcttcgcg ctgattgtaa
     2881 ttatgtccgt gacgccgtgg agttcggtag tcccggagaa aagcccgttt gttgaactgt
     2941 tcgtgttggt agggctgcct gctgccgcaa gcgtgatcaa ctttgtggtg ctgacctctg
     3001 cggcgtcttc cgctaacagc ggcgtcttct ctaccagccg tatgctgttt ggtctggcgc
     3061 aggaaggtgt agcaccgaaa gcgttcgcta aactttctaa gcgcgcagta cccgcgaaag
     3121 ggttgacctt ctcgtgtatc tgtctgctcg gcggcgtggt gatgttgtat gtgaatccta
     3181 gcgtgattgg cgcgttcacg atgattacaa ccgtttccgc gattctgttt atgttcgtct
     3241 ggacaattat cctttgctcg tacctggtgt accgcaaaca gcgtcctcat ctgcatgaga
     3301 agtcgatcta caagatgccg ctcggcaagc tgatgtgctg ggtatgtatg gcgttctttg
     3361 tgttcgtggt cgtgttgctg acactggaag atgacactcg ccaggcgctg ctggttaccc
     3421 cgctgtggtt tatcgcgctg gggttgggct ggctgtttat tggtaagaag cgggctgctg
     3481 aactgcggaa ataaccgcat tatcatgctg gatggcgcaa tgccatccag cttttagatc
     3541 actcacccgc cagcgcgcgt gggaacagta cattgttttc caggctgatg tgatccatca
     3601 ggtcatcaat cagttcatta atgccgttat acatcgcttt ccaggtggtg caggcttccg
     3661 gcggcggtgt gacgttattg gtagtgtgtt tgattacttc cagcagttca cccgcttcat
     3721 cgtgctcgct ttccattacg ctgattggcc ccattgcctg gctgcccatg ccttgtttga
     3781 tcatcgggaa gaggatctgc tcttctttca tcatgtggct ggaaagctct tcatgcagca
     3841 tagtcaggta ttttgtcagc ccttttggca cgctcggttt gtcggcgtga acgcgctcga
     3901 ctttagtcgc ttgcaggatc agctccggca actgctcgcg gtgacgatcg tggtagcgca
     3961 cgatgatatg gtcaatgatt tctgccagcg gggcgctacg ccagtctttc tcaatcggtt
     4021 gttcagcgag ctttgccagt tcagcttcaa tgacctcaac atccagttct ttacgtgccg
     4081 ccgcgcgcgc cagcgtctgc ttaccgccac agcagtaatc catatcatat ttacgaaaca
     4141 gagctgaagc gcgaggaata gagagcgcca gttcacctaa aggttggtcg cgataagcca
     4201 tagctgatac ctcattctta ataagataag atgtatttta aatgcatctt taaggcaaaa
     4261 gctataaccc ttacgtagca gaggcttatt taactcactg ccagcgtgtc aactttctga
     4321 gacagattca gttaaaggtt tttctggttt tgctctgacc gcgatgacca cgccaatcac
     4381 taacagcgcg ataccgctca gcgtcattag cggcggcatt tgctggcgga gtaaaaaggt
     4441 gtacagcaaa cctgccagcg tttcgaaaac aatcagcggc ccgagaatca ctgtcggtaa
     4501 tcgctggctg gcgacgttcc agcagagtgc gccaacccat gagcaaagca cggctatcgc
     4561 aatcatcaga ctaataaaca ccagcggacg ggggccaaag ggtagggaga agtccggcgt
     4621 ttgcgtattc agccagtaac aagcgacgag atagccgata agtgaaaccg gcagtgtgac
     4681 cagcgcctgc gctgttgccc acatcatcgg atgtttgtcg ggattttccc gcagccagcg
     4741 ggcgttgcgc agggcatacc acgcccagca gaccacggaa actaacgcta gcacgatgcc
     4801 agaggtataa cgtgcccagt caaaatcggg gagtccgtgg tttaactcag caatattcac
     4861 actcgcgagg ccgatgccaa tacaaatcag tgccggggcg agttttcccc acgcgagttt
     4921 gccgtcgcgc tggctataaa gcagattggc aaagacagga atgaccaccg gcagggtgcc
     4981 gataatcatc gtggaaacag gcgcgccagt acgttgaatg gcactggcaa ggcagaagta
     5041 ataaatgagg ttgcccatca tagtgagcat caaggcggta agccagtcgc gacgcgccaa
     5101 ctgacgcaga cgcacgcgtc ccagccaggc aatgggcagc gcaattaacc ctaacgccag
     5161 ataacgcccc atcgactgca acatcgccgg gtattccggc acgatcaacg gcccgacaaa
     5221 aataagcccc cacatcaacc ctgctaacag ggcgtacagc acgccgctaa tcattactgg
     5281 catccattga tctgtcagaa gaaagttcag gaagcagaca tgattgctcc cggcatcccg
     5341 tcattatcag agaggatgcg ggaggatgcc actttaatta actagttatt aacattaaaa
     5401 agatggctta cgctttcggc taacgttgtc gttgggtggc caatcagttt gctaagcgtt
     5461 ttgctgtcat caaacagacc gcctttcgat gcgccaacgt cagaatccgc cagcatatcc
     5521 gccagtccgt cgggcagtcc gacgcttttc agtgccgcgg cgaaatcggc ttcgctcaga
     5581 ttttgatagg taacggattt tccgctctgt ttggtcagct ctgccgctaa ctgtgtcaac
     5641 gtccaggcac tatcgccagc cagttcgtaa accttccctt cgtgaccgac ttcgctaatc
     5701 acgcgtgccg cagctgccgc ataatctgcc cgcgttgctg aggcgatttt gccatcgccc
     5761 gccgcaccga taaatacgcc gtgttccagt gctgccgggg cgctggcgag gtagttttcg
     5821 ctgtaccagc cgttgcgcag cagggtgtaa acgatgccag aatcagccaa cattttctcc
     5881 gtctcaatgt gctcatcggc gaggccgagc ggggaggtat ctgcatgtag caggctggta
     5941 taagcgataa atttcacgcc agccgtcttt gcggcattaa taacattacg atgctgcggg
     6001 gcacgttgac ccacttcgct ggaagatatc agcagtagtt tttccactcc ctgaagtgca
     6061 gatgtcagtg cggcttcatc gccgtagtca gcctgacgca cggtaatgcc ttgtgctgcc
     6121 agggcctggg cttttgccgg attacgaacg atagccacta tttggctggc aggaaccgtt
     6181 ttcatcaagg attcaataac atagtgacca agttggccag tggcaccagt aatagcgatc
     6241 atgggaagtc tccatcgttt tgtcttgtgt ttaaatacgc taacacctaa acttactttt
     6301 agtaagtaca tacaaaaagg taagtatgaa atgagtcagg ttagcctgtc gcaacaactg
     6361 aaagagggca atctctttgc ggaacagtgc ccgtcgcgcg aggtgttgaa acacgtcacc
     6421 agccgttggg gggggttgat tctggtggcg ctacgcgaag gtactcatcg ctttagcgac
     6481 ctgcggcgca aaatcggcgg ggtgagcgaa aagatgcttg cgcagtcgtt acaggcgtta
     6541 gaacaggatg gttttcttaa ccgtatcgcg tatccggtgg tgccaccgca tgttgaatac
     6601 agcctcacgc cgctgggcga gcaggtgagc gaaaaggttg ctgcactggc ggactggatt
     6661 gagttgaatt tgcccgaggt gttggcggtg cgggatgaac gtgcggcata actgccggat
     6721 gcgcggccta cggtgtatga cttgtagggc ggataagatg cgtcagcatc gcatccgcca
     6781 ataaatgccg gatgcgattg atgatttata ggccgaaaaa gaagtgtttt acttactcaa
     6841 atccacctga taaatcgcaa acccgatatc atcggtcgcg actttattca tcggatactg
     6901 ccctttctct ttaataaacg ctgcggcttt atctgacggg gaagtttcga aacggatatc
     6961 cagtttctta tcggcagcta tcggtgctaa acgccagttg ttatctgccg ccgggtgaat
     7021 ttcccccgca cgtttcgact catcagcaat ccacgctgcc agaaccgagc ggttctcatc
     7081 cggtgaagca aaagcgatat ggctgtcgcc cgtcccggca aatttgccgc cgtaagcgcg
     7141 atagttattg gtggcaacga ggaacatagc gttcggatca atcggcttgc cattaaaggt
     7201 caggttctta atcctttccg cattggcatt aatcatctgg cactcgccgt cataacgggc
     7261 gggctgggta acatcaatct gataattcac accatcaata acatcaaagt tataggtgcg
     7321 gaaaccgtcc cagttgatga gtgactgtgg tttcgtgctg ttgggatcaa tctggttaaa
     7381 ctgtccggcg gagcactcca gccactcttt cacctcttta ccgctggctt tcaccacaat
     7441 cagcgtattg ggatagagat aaagatcggc ggcattacgg aaggtcaact ggcctttttc
     7501 cacctccaca tagcttgccg ggtcattttt gcgaccacca actttaaacg gtgcggcagc
     7561 tgaaagcacc ggcagttttg ccagatccgg atcgccctga atgtaatgtt cgacatacgc
     7621 tttttgcgcg ttgttcacta cttgcacggt cggatcgtcc tgcaccagcg ccagatagct
     7681 atacatgttg tcggcagatt taccgattgg cttgctgacg aactggcgtg tggcatcgtg
     7741 atcggctttg agtgtttcta ccagcttgct gtcttccgcc gcgagggatt ttttattggc
     7801 gatgtcgtaa atcggccgtg cttccgcttt cgcctgcgtc acctgccatt taccgctgtc
     7861 attactgagt tgtaagtcga ccaccccaag atgatcgccc cacatgcccg gcattaccgc
     7921 cggaacacca ttcagcgtgc ctttggtgat atcagcccct tcgatatcag caaaatcttt
     7981 acccgggaaa acggcgtgag catggccaaa cataatggcg ttaacgcccg gaatttcact
     8041 gaggtaataa actgagtttt ccgccatcac tttatacgga tcggcagaca gcccggaatg
     8101 tgccagaacg acaacaacat cggcaccttt ctcgcgcatt tcaggcacgt atttgcgcac
     8161 ggtttcggta atatcattca ccgtcacttt cccggataaa ttagctttat cccagcccat
     8221 gatttgtggc ggcacgacgc caatatagcc aatcttcagc gtctgttttt ttccgtcttt
     8281 atcgaccact tcggtgtctt taattaaata cggtgtaaac attggctgtt tggttctggc
     8341 gtcaatgacg ttggcattta cataagggaa tttcgctcct gccagcgcat ttttcaggta
     8401 atccagaccg tagttaaact cgtggttgcc aagcgttccg acggtatagt ccagcgtatt
     8461 taatgcctta tagaccgggt gaatatcacc tgcttttaat cctttcgccg acatgtaatc
     8521 ggccagcgga ctcccctgaa tcaaatcgcc gttatcgacc agtacgctgt ttttaacttc
     8581 attgcgggca tcgttaatca ggcttgccgt acgtaccagt ccgaattttt ccgtggcggt
     8641 gtctttgtaa taatcgaaat ccatcatgtt gctatgcaga tcagtggttt ccatgatacg
     8701 cagatcgacc gtcgctgcat tcacactggc ggcaatcagc gtggccagga gcgttgcgct
     8761 aaacttaatc atcagggaca tccttttatc atcgggaata cgaaagaaaa gggagaataa
     8821 acgtcttact tatagaacag tgaagaatgc cacaatttta cgctttgaaa atgatgacac
     8881 tatcacagtt ggcgcattca ttaacgatag ggtataagta aaacaataag ttaacaccgc
     8941 tcacagagac gaggtggaga aatgttagat caagtatgcc agcttgcacg gaatgcaggc
     9001 gatgccatta tgcaggtcta cgacgggacg aaaccgatgg acgtcgtcag caaagcggac
     9061 aattctccgg taacggcagc ggatattgcc gcacacaccg ttatcatgga cggtttacgt
     9121 acgctgacac cggatattcc ggtcctttct gaagaagatc ctcccggttg ggaagcccgt
     9181 cagcactggc agcgttactg gctggtagac ccgctggatg gtacgaaaga gtttattaaa
     9241 cgtaatggcg aattcaccgt taacattgca ctcattgacc atggcaaacc gattttaggt
     9301 gtggtgtatg cgccggtaat gaacgtaatg tacagcgcgg cagaaggcaa agcgtggaaa
     9361 gaagagtgcg gcgtgcgcaa gcagattcag gtccgcgatg cgcgcccgcc gctggtggtg
     9421 atcagccgtt cccatgcgga tgcggagctg aaagagtatc tgcaacagct tggcgaacat
     9481 cagaccacgt ccatcggctc ttcgctgaaa ttctgcctgg tggcggaagg acaggcgcag
     9541 ctgtacccgc gtttcgggcc aacgaatatt tgggataccg ccgctggtca tgctgtggct
     9601 gcggctgccg gagcgcacgt tcacgactgg cagggtaaac cgctggatta cactccgcgc
     9661 gagtcgttcc tgaatcctgg gttcagagtg tctatttatt aattaattta tgcagcaggt
     9721 ccatcacttg ctgcacctct tcctgagtaa gcgccccgtc tttggcccat tgcacgcgac
     9781 cgtctttatc cagcaccact acagcggaac ttttttcatc cagctgccag gcaccgcgtg
     9841 cgacgccatt gctatcaaca ataaactgcg accagggata aagcttttta ttactctcca
     9901 gactgctgcg cacaaacatg ccggaacccg gaattgcgtc gtcggtgtta acaatggtgg
     9961 tggtctggta acgatcgtgc ggtaacttcg ctgatttaat cgcttcaatc agcgtcgcgt
    10021 ttttctcttt tgcagaggtg cgaccagcaa tatgttgcag tactccgcac ttttcccact
    10081 aactgcgcgc tgttccaggt tttgtagcta aactgatctt tatcaagcac caactcgcca
    10141 cgatcggtga tgccaatcgg cggcactcgc tgaccggttt cgaactgatg tgcggaagcc
    10201 atcatcggca acagcaggca ggtgagtgcc agaatcttgc gtagggtcat ggtgtttcct
    10261 tcttatgata tgcaggtgat ccgaccactt gggtctgagt tttaatcata tgtgctattt
    10321 atcgaatttc ccgcaagtgt gatgccagtt tgcggtcaag cgcacaaatc atatgaaaaa
    10381 tgaatgctta tactgaagac cgcgcttcgg taaaaagata attctgaata attgtaacct
    10441 ttaggtaaaa aagttatacg cggtggaaac attgcccgga tagtctatag tcactaagca
    10501 ttaaaatttg cgcctcataa tagttgggcc gattgtggca ccgcacaggc gtaatactca
    10561 gcaggagata acaatgaaaa ttttccaacg gtacaaccca cttcaggtgg cgaagtacgt
    10621 aaagatcctg ttccgtggac ggttatacat caaggacgtt ggcgcttttg aatttgataa
    10681 gggtaagatt cttatcccaa aagtgaagga taagctgcat ttgtcagtga tgtccgaagt
    10741 taaccgtcag gttatgcgtc tgcaaacaga gatggcttaa ccaaagtgct atgcagtaat
    10801 aagacggctc ctgattcagg agccgttgat gtttctgggg gctacgcgac gctttcttct
    10861 ttatctttag cgtcaggcag ttttggcgaa agggcggtgg ccttgctgtc gatccgggtc
    10921 accagcagct ggtcgatgcg gtagttatcg atatccacca cttcaaattt gtagccggcg
    10981 aatttcaccg aatcggtgcg tttcgggatc ttacgcagca taaacatcat aaagccgccg
    11041 atggtttcgt agttgcccga ctgcgggaac tcgtcaatat ccagcacgcg catgacgtcg
    11101 tcaatcggag tgccgccgtc aatcagccat gagttctcat cacgggcgac aatctgctct
    11161 tccagcccct gaccaaccag atcgcccatc agcgtggtca tcacgtcgtt gagggtgatg
    11221 atccccacca ccagcgcgta ctcgttcatg atcaccgcga agtcttcacc tgcggtttta
    11281 aaactttcca acgcctctga aagggttaac gtatccggca caatcagcgt gttgcgaatt
    11341 tgtacgccgc tgttcagtgc caggctttgg ttagccagca cgcggttcag caggtcttta
    11401 gaatcgacat aaccgatgat gtggtcaata tcttcattac agacgaggaa cttagagtgc
    11461 ggatgttccg ccaccttatt cttcaggctt tgctcatctt cgtggagatc aaaccaaatc
    11521 acgttttcac gcggtgtcat tgaagacgga acggtacggg attccagctc aaagacgttt
    11581 tcaatcagct cgtgttcctg tttacgtaac acgcctgcca gcgcaccggc ttccactacc
    11641 gcgtagatgt catcagaagt gatgtcatct ttacgtacca ttggcagttt gaaaatacgg
    11701 aagattatgt tcgccagtcc gttgaagaac cacaccagcg gggtgcaaac gtacaggcag
    11761 aagcgcatcg ggttgatgat acgcaaagcc accgcttctg gcgcaatcat accgatgcgt
    11821 ttcggggtta aatccgcaaa caggataaac atgccagtca ctaacgagaa agagagaata
    11881 aagctcagtt gctcagagag ttctgccgac atatagcggg agaacaggct gtgaaaagct
    11941 ggagaaaatg ccgcatcacc gacgataccg ccgagaatcg ccactgcgtt cagaccgatt
    12001 tggaccacgg taaagaacat gccgggattt tcctgcatat tcagaacgcg ttgggcattt
    12061 atattgcctt catcagccag cagtttaagt ttgattttgc gtgaggcggc aagcgagatc
    12121 tcggacatcg agaagaacgc acttacagcg atcaaacaga gtatgactaa aatactgttt
    12181 aacatatctt atccgacctt tcaggccaga tcctcggaag gggaagttga ttaatttgtg
    12241 tgaaatacac attgaaagcc gattcgaaga aagtgaaccg gcattttcag tggctagtat
    12301 agcgtaaggt actgtaaagt cgccagaggg ttaaaattca gactgctgat aaatgcaata
    12361 ctgcctgatg cgctatgcc
//
LOCUS       DAZGXZ010000140        12366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000140 DAZGXZ010000000
VERSION     DAZGXZ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.140"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(41..322)
                     /locus_tag="ACHKJF_004271"
     CDS             complement(41..322)
                     /locus_tag="ACHKJF_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP0951526.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(585..1694)
                     /gene="apbC"
                     /locus_tag="ACHKJF_004272"
     CDS             complement(585..1694)
                     /gene="apbC"
                     /locus_tag="ACHKJF_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP0951527.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1826..3859
                     /gene="metG"
                     /locus_tag="ACHKJF_004273"
     CDS             1826..3859
                     /gene="metG"
                     /locus_tag="ACHKJF_004273"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP0951528.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEDRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4000..7794
                     /locus_tag="ACHKJF_004274"
     CDS             4000..7794
                     /locus_tag="ACHKJF_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFP0951529.1"
                     /translation="MRHFIYQDEKLHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDNALLHLGEMLRFPQEEALYPGLLQVKDACTADSLAEFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7804..11436
                     /gene="yehI"
                     /locus_tag="ACHKJF_004275"
     CDS             7804..11436
                     /gene="yehI"
                     /locus_tag="ACHKJF_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP0951530.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11497..11817
                     /gene="yehK"
                     /locus_tag="ACHKJF_004276"
     CDS             11497..11817
                     /gene="yehK"
                     /locus_tag="ACHKJF_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP0951531.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
BASE COUNT         3229 a         3152 c         3022 g         2963 t
ORIGIN      
        1 atttcggctt tctaaatgca attaaaatca ggttgccaaa ttaatcgtag cgatttaaca
       61 cgattcgacg tttgctatcg ctacctgcaa caaccacttc tgtcgttaca cctttgacgg
      121 gcgaagaaaa cagatgacgg gtgtcaacat tatacgttaa ctgaccacaa tgctgttgag
      181 gttgaccatt aaccatagcc atactgcatg ttgggggact gatcctgcca ttaatcgcta
      241 atgtggcagt ctctgaagag aatgcgggag aagccaatcc ataggcaaga aaaataattc
      301 ctgacagcca atacttattc attgaacgtt atccctgtag taaaggttat gcctggatag
      361 aatgagtgca taacaaacta tagctgtaca tccactacac agccacgaag gatgataatg
      421 aagcattgct tgtatgatca atcgactttg tagagtttcg gacgaaggtc cgcagaatat
      481 tcgcagtatt aaataagtgt tcattaatgc ggcaaaaact aatacaccgc atcaatgtaa
      541 catctctgcg gtgtatgaag aataaccaga atggttaata ggcgttagac cgcgcggaag
      601 gaaatctcgc ctggaatgac ttcaccctgc cagtagagct gagctgcaac gcggtcagcc
      661 agctggcggt agatagcggt aaattcgctc tccggacggc tgataaccgt tggcgtgcct
      721 ttatcgagat cctcacgtaa ggagatgtgt aacggcatct gccccagcag ctgtgtgtgg
      781 tatttttcag ccagtttctc agcaccaccg gtaccgaaaa tcggttcatg gtgaccacag
      841 ttactgcaaa tatgcacact catgttctcg acaataccaa gcaccggaac ctcgactttc
      901 tcgaacatca caatgccttt tttcgcatcg atcaacgcga tgtcctgcgg ggtggtgacc
      961 acgactgcac cagttactgg aatgttttgc gccagcgtca attgaatgtc accggtgcct
     1021 ggcggcatat caagcaccag atagtcgaga tccggccaca aggtttcctg caacatctgc
     1081 attaacgcct tgctcgccat cggtccacgc cacaccatcg cattgtcgtc agtgaccaga
     1141 taaccaattg aattggttgc caggccatga gacataatcg gagccatgtg ggtaccgtcc
     1201 ggcgacgttg gacgctgatt ttctgcgccc agcatggtgg gaattgatgg gccgtagata
     1261 tcggcgtcca gaatgccgac tttcgcccct tccgccgcca gtgccagcgc caggtttacc
     1321 gccgtggagg atttacccac gccgccttta ccggagctga ccgcaatgat gttcttcaca
     1381 ccattaatac ccggctgatt cttcacgcgt tttaaggtgg cgatattgtg cgacagtttc
     1441 cagtcgatag ctttcgcccc ggtgatacgc agcagttcgg cgctacattg ctcttttaac
     1501 tcttcaaacg cgctatgcca gacgaatggc ataaccagtt ccacatgcaa cgtgtcgtcc
     1561 atccaggcga catggtgcaa cgctttcagc gtggtgaggt tatgcttcag ggttgggtgc
     1621 tgaaaattgg ccagtgtccc ggcgaccatt gcgcgcaagg cttctggtga tttggcctgg
     1681 gattgttcgt tcatcccgac tccttttttg tatagataaa ccatcagctg atagtttacc
     1741 tgaagaatat agagaagtac ttacttaaca ttttcccatt tggtactatc taatcccttt
     1801 tcactattaa gaagtaatgc ctactatgac tcaagtcgcg aagaaaattc tggtgacgtg
     1861 cgcactgccg tacgctaacg gctcaatcca cctcggccat atgctggagc acatccaggc
     1921 tgatgtctgg gtccgttacc agcgaatgcg cggccacgag gtcaacttca tctgcgccga
     1981 cgatgcccac ggtacaccga tcatgctgaa agctcagcag cttggtatca ccccggagca
     2041 gatgattggc gaaatgagtc aggagcatca gactgatttc gcaggcttta acatcagcta
     2101 tgacaactat cactcgacgc acagcgaaga gaaccgccag ttgtcagaac ttatctactc
     2161 tcgcctgaaa gagaacggtt ttattaaaaa ccgcaccatc tctcagctgt acgatccgga
     2221 aaaaggcatg ttcctgccgg accgttttgt gaaaggcacc tgcccgaaat gtaaatcccc
     2281 ggatcaatac ggcgataact gcgaagtctg cggcgcgacc tacagcccga ctgaattgat
     2341 cgagccgaaa tcggtggttt ctggcgctac gccggtaatg cgtgattctg aacacttctt
     2401 ctttgatctg ccctctttca gcgaaatgtt gcaggcatgg acccgcagcg gtgcgttgca
     2461 ggagcaggtg gcaaataaaa tgcaggagtg gtttgaatcc ggcctgcaac agtgggatat
     2521 ctcccgcgac gcgccatact ttggttttga aattccgaac gcgccgggca aatatttcta
     2581 cgtctggctg gacgcaccga ttggctacat gggttctttc aagaatctgt gcgacaagcg
     2641 cggcgacagc gtaagcttcg atgaatactg gaagaaagac tccaccgccg agctgtacca
     2701 cttcatcggt aaagatattg tttacttcca cagcctgttc tggcctgcca tgctggaagg
     2761 cagcaacttc cgcaagccga ccaacctgtt tgttcatggc tatgtgacgg tgaacggcgc
     2821 aaagatgtcc aagtctcgcg gcacctttat taaagccagc acctggctga atcattttga
     2881 cgctgacagc ctgcgttact actacactgc gaaactctct tcgcgcattg atgatatcga
     2941 tctcaacctg gaggatttcg ttcagcgtgt gaatgccgat atcgtgaaca aagtggttaa
     3001 cctggcctct cgtaatgcgg gctttatcaa caagcgtttt gacggcgtgc tggcaagcga
     3061 actggctgac ccgcagttgt acaaaacctt cactgatgcc gctgaagtga ttggtgaagc
     3121 gtgggaaagc cgcgaatttg gtaaagcagt gcgcgaaatc atggcgctgg ctgatctggc
     3181 taaccgctat gtcgacgaac aggctccgtg ggtggtggca aaacaggaag accgcgatgc
     3241 cgacctgcag gcaatttgct caatgggcat aaacctgttc cgcgtgctga tgacttacct
     3301 gaagccggta ctgccgaaac tgaccgagcg cgcagaagca ttcctcaata cagaactgac
     3361 ctgggatggt atccagcaac cgctgctggg ccacaaagtg aatccgttca aagcgctgta
     3421 taaccgcatc gatatgaagc aggttgaagc actggtggaa gcgtctaaag aagaagtgaa
     3481 agcagctgcc gcgccagtta ctggcccact ggcagatgat ccgattcagg aaaccatcac
     3541 ctttgacgac ttcgcaaaag ttgacctgcg cgtggcgctg atcgaaaacg cagagtttgt
     3601 tgaaggttct gacaaactgc tgcgcctgac actggatctc ggcggtgaaa aacgcaatgt
     3661 cttctccggt attcgttctg cctacccgga tccgcaggct ctgattggtc gtcacaccat
     3721 tatggtggct aacctggcgc cgcgtaaaat gcgctttggt atctccgaag gcatggtaat
     3781 ggctgctggt cctggcggga aagatatctt cctgctaagc ccggatgccg gtgctaaacc
     3841 gggtcatcag gtgaaataat cccccttcaa ggcgctgcat cgacagcgcc ttttctttat
     3901 aaattcctaa agttgttttc ttgctatttt gtctctctct aacccgcata aatactggta
     3961 gcatctgcat tcaactggat aaaattacag ggatgcagaa tgagacactt tatctatcag
     4021 gacgaaaaat tacataaatt ctgggcggtt gagcaacagg gaaacgagtt gcatatcagt
     4081 tggggaaaag ttggcaccaa agggcaaagc cagataaaaa gtttttcaga tgctgcggca
     4141 gtggcaaaag cggagcttaa gctgattgcg gagaaggtga agaaggggta tgtggagcaa
     4201 gcgaaggata attctttgca accttcccaa acggtaacgg gctctctcaa ggtagcggac
     4261 ttatccacca ttattcagga acaaccctct ttcgtagcag aaacccgtgc gcctgacaaa
     4321 aatacagatg ctgttttacc gtggctggcg aaagatattg ctgtcgtttt tccgcccgaa
     4381 gttgtacaca ccacgttaag tcatcgccgc tttcccggag ttcctgttca gcaagcagac
     4441 aaattgaccc aattacgtcg cttagcctgt agtgtgtcgc aacgggataa taaaacagcc
     4501 acatttgact tcagcgcctg ttctttagaa tggcaaaaca ccgtcgccca ggcgatcagt
     4561 cagatcgacg gcctgaaaac aacacagcta ccatcaccag taatggctgt actcacggca
     4621 cttgaaatga aatgcacaag atataaagtg cgtgaggatg ttatggatca gatcgtccag
     4681 gaaggcggtc tggaatatgc tactgatgta ataatacacc ttcaacagat tgatattaaa
     4741 tgggattatg cgaataatgt cattattatt ctgccgtctg gcattgcacc tgactacttg
     4801 gagcaatatt ccagatttga attacgccta cgtaaacatt tatcactggc ggaagagtct
     4861 ctctggcaaa aatgtgcaca aaaacttatt gccgcaattc cacatattcc agaatggcgg
     4921 caaccattaa ttgctttgtt attacccgaa aaaccagaaa ttgcacatga aattgcccag
     4981 cgtctactgg ggcaaaaaaa attaccctcg cttgagtggt taaaaatagt ggcgactgat
     5041 gagcacattc ttgcctcatt agaaaaatat cacgagccat atgccatttt tgatgattac
     5101 tattgtggtg cgatatggtc agccaccgta ttacaggagc aaggtgttgc agccctgccc
     5161 cgatttgctc cctatgccgc aagtgactac tgcgccgatg tgttgcgtca tatcaatcat
     5221 ccgttcgcat taacactgct tatacgtgta gccgggcata ctaaacgctg tcacgatcgg
     5281 atgacgaaag cctgcgctgc gttcccacat gcagcaatgg cggcactgac ggaacttctt
     5341 gggcaaaaag aagagaacag ttggcgcatt atgctaatga caatgcttat ctcacaacca
     5401 gcactggcag aacaggtcat tccctggctc tcgacacccg cagttgccgt actgaaatca
     5461 tgccagcaac aactgacaca gccctcaaac catgccagcg ccgatctact gccagccgta
     5521 gtagtctccc ctccctggct ttcgaaaaag aaaaaatcgc cgattccggt gctggattta
     5581 gcgccattag gcattgagcc aatctgttat ctgacagaag aaatcagtaa tcaacttttg
     5641 gcgaaatata tctggtattc aaaacacatc acggttagcc atgaagaaag tactaccaac
     5701 ctgttggcaa ggatgggttt tcaacgacgg atcgctggta catatattaa agctcccgaa
     5761 gcggtagttg aggcatggct aaatgaagat tattcaacct tactaagtga atttaaggtg
     5821 tttcattcac ctaccgggca ttattggcag ttggggattt tgacaacatt gccgctggag
     5881 aaagcagtaa aagcatggaa tgcccttacc ctatctccac ataccgatac cgaatacgcc
     5941 atgttacatt ttggactcaa agggttacct gggttagtaa actcacttgc acgctatcca
     6001 caagaagcct tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc
     6061 cgtgccttca acaaactgaa aacgctacgc gaaaacgccc gtagctggct gttgaaatac
     6121 ccggaacatg cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacag
     6181 gataacgccc gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa
     6241 gaaatcgccc gacgttataa ccagccggaa gtaaccgatg cggtgaacgc tctgcttgcg
     6301 ctcgatccct tagataatca cccgacaaaa atccccactc ttccggcctt ttatcagcca
     6361 tcgctctgga cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc
     6421 ctccacctcg gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg
     6481 caggtgaaag atgcctgtac cgccgactca ctggctgaat ttgcctggga tctgtttacc
     6541 gcctggcaga ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt
     6601 ctcggtaacg atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa
     6661 tcccagcata aacgcgccac cgttgggttg gatattctcg ctgctatcgg tagtgatatc
     6721 gcccttatgc agcttaacgg catcgcccag aaactgaaat tcaaagcatt acaggagcgg
     6781 gcaaaagaaa aaattgccga cattgccgag agccgcgaac tcacggtggc ggagcttgaa
     6841 gatcggttag caccggatct cggtctggat gataacggtt cgctgctgct ggattttggc
     6901 ccacggcagt tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc
     6961 ggcagccgcc tgaaagacct gcccaaaccg aacaaaagcg atgatgaaac gcgggcgaac
     7021 gatgcggtta accgctacaa attgctgaaa aaagatgcgc gtaccatcgc cgcccagcag
     7081 gtagcaaggc tggaatccgc catgtgcctg cgccgccgct ggtcgctgga aaacttccag
     7141 ctcttcctgg ttgagcatcc gctggttcgt cacttaaccc gccgtctgat ttggggcgtt
     7201 tatagcgccg aaaaccagct actggcttgc tttcgcgtag cagaagataa cagctacagc
     7261 accgctgacg atgatctttt caccctgccg gaaggcgata tctctatcgg cactcctcac
     7321 gttctggaaa tatcaccaac ggatgctgcc gcctttggtc agctttttgc cgactacgaa
     7381 ctgctaccac cgttccgcca gctcgaccgt aacagctacg ccctgacaga agccgagcgc
     7441 aatgccagtg aactgacccg ctgggcaggc agaaaatgcc cgagcggtcg ggtcatggga
     7501 ctggcgaata aaggttgggt gcgtggtgaa ccgcaggatg gtggctggat cggctggatg
     7561 atcaaacctc tgggccgctg gtcgttaatc atggaaatcg atgaaggctt tgcagttggt
     7621 atgtcgccag ccgaacttag cgccgagcaa ctcttaagca agctgtggct atgggaaggc
     7681 aaagcagaaa gctatggctg ggggagtaat tcaacacagg aagcgcagtt ctccgtactc
     7741 gatgccatca ccgccagcga gctgattaac gatattgaag ccctgtttga ataaggaaag
     7801 agcatggaca aggaattacc gtggctggcg gataacgccc aactggaact gaaatataaa
     7861 aaaggcaaaa caccactcag ccatcgcaat tggccgggcg aaccagtgcc tgttatcact
     7921 gaaagcatca ttcagacact gggtgatgaa ttgctacaaa aagctgagaa gaaaaaaaac
     7981 attgtctggc gttatgagaa tttttcactg gagtggcagt ccgccattac gcaggccatc
     8041 aacttgatcg gcgaacacaa accctcaatc ccggcccaga caatggcggc gctagtctgt
     8101 atcgcgcaaa atgacagcca acagttgctc gacgaaatcg tccaacaaga ggggctggaa
     8161 tatgcgactg aggtggtgat tgcacgccag tttattacac gatgttatga gagtgatcct
     8221 ctggtagtca cattgcagta tcagaacgag gactatggct atggttatcg ctcagaaacc
     8281 tataacgaat tcgatctccg actgcgtaag catctctctc tggcagagga aagctgctgg
     8341 cagcgttgcg ccgacaaact cattgccaca ctgccaggaa taaccaaagt tcgccgccct
     8401 tttattgcgc tgatcctccc cgaaaaacca gaaatcgcca atgagttggt aagccttgaa
     8461 tgcccgcgga ctcattttca ttctaaggag tggttaaaag ttgttgctaa tgaccccaca
     8521 gcggtgagaa aactcgaacg ctactggagc caggatatat ttagcgatcg agaagccagc
     8581 tacatgtcgc atgaaaacca cttcggctac gcggcctgcg ccgccctttt gcgcgaacaa
     8641 ggactggcag ccgttccgcg cctcgcgata tatgcccata aagaagattg cggcagtctg
     8701 ctggtacaaa ttaaccatcc gcaagtcatc cgcaccttgc tgttagttgc tgataaaaac
     8761 aaacccagcc tgcaacgtgt ggctaaatac agtaaaaact tcccccatgc gacactcgcc
     8821 gcactggcag aactgctggc gttaaaagaa ccaccagccc gcccaggtta tccaatcatc
     8881 gaagacaaaa agctgcctgc acagcaaaaa gcacgcgatg aatactggcg tacgctgtta
     8941 caaacgctga tagcatcgca gccacaacta gcagaaaggg tgatgccgtg gttaagtact
     9001 caagccaggg cagtgctgaa tagttattta tcggcaccgc caaaaccggt tattgatagc
     9061 accgataaca gcagtctgcc tgaaatgctc gtttcaccac cgtggcgtag taagaaaaaa
     9121 atgacagctc cacgtcttga tttggcaccg ctcgaattaa ctccgcaaat ttactggcaa
     9181 ccaggcgaac aagagaggct tgccgccact gagtctgccc gttatttcag cacggaatct
     9241 cttgcggaac gcatggaaca aaaaagtgga cgagttgtat tacaggaact gggttttggg
     9301 gatgatgtat ggctgtttct gaattatata ctccccggaa aactggatgc tgcacgcaat
     9361 tcactcattg ttcagtggca ttactaccag gggcgggttg aagagatcct gaatggctgg
     9421 aactccccgc aagcacaatt agcagaacag gcgctccgca gcggtcacat agaagcgtta
     9481 attaacatat gggaaaatga caacttctca cgttatcgtc cggaaaagag tgtctggaac
     9541 ctgtatttat tggcacagtt gccgcgtgag atggctttga ccttctggct gcgtatcatt
     9601 gagaaaaagc atctgttcgc gggtgaggac tattttctca gtatcctcgg attggatgcg
     9661 ctaccaggtc tgctgttggc tttttcacat cgtccaaaag aaacatttcc gttaatttta
     9721 aattttggcg caacagaact ggcgctgcct gttgcccgag tctggcgacg tttcgcggcg
     9781 cagcgtgatc tggctcgcca gtggatttta caatggccgg aacatacggc tagtgcactt
     9841 atccctcttg tctttaccaa acccagcgat aatagcgaag ccgcattact tgccctgcgt
     9901 ttactgtacg aacagggaca tggcgaattg ctacaaaccg tggcaaaccg ctggcagcgt
     9961 acagatgtat ggtctgccct ggagcagttg cttaaacagg gtccaatgga catttacccg
    10021 gcacgcattc caaaagcccc tgatttctgg catccggcaa tgtggtccag gcctcgactt
    10081 atcactaata atcagcctgt taccggtgac gctctggaaa ttatcggcga aatgctgcgc
    10141 tttacccagg ggggacgttt ttatagcggg ctggaacaac tgaaaacgtt ctgccagcca
    10201 caaacgctgg cagcttttgc ctgggatctc ttcactgcgt ggcaacaagc tggtgccccc
    10261 gcaaaagaca actgggcatt tctggcgtta agtctctttg gtgacgaaag cacggcacgg
    10321 gatctgacga cacagatcct cgcctggcca caagaaggca aatctgcccg tgctgtcagc
    10381 ggcctgaaca tccttaccct gatgaataat gatatggcgc tgatacagct gcatcatata
    10441 tcgcaacggg ctaaatcccg ccccttacgt gataacgcgg cggaatttct tcaggtggtc
    10501 gcagaaaatc gcgggctaag ccaggaagag ctagcggaca gattagtccc aaccctgggc
    10561 cttgatgatc cgcaggcgtt gagttttgat tttggtcccc ggcagtttac cgttcgcttc
    10621 gatgaaaacc tcaacccggt tatctttgat cagcaaaacg ttcgccagaa aagcgttccc
    10681 cggttgcgcg ccgatgacga tcaactgaaa gcgcccgagg cactggcccg actaaaaggg
    10741 ctaaaaaaag atgctactca ggtgagcaaa aacctgctcc cgcgtcttga agctgcccta
    10801 cgtaccaccc gacgctggtc gctggcagat tttcatactc tgtttgttaa tcatcccttt
    10861 acccgcctgg ttacccagcg attaatatgg ggcgtgtatc cggcaaatga accgcgtcgt
    10921 ttactcaacg cctttcgtgt ggccgcagag ggggagttct gcaatgcgca ggatgagcca
    10981 attgacctgc ctgcggatgc tctgattggc attgcccacc cgttagaaat gacagcagaa
    11041 atgcgcagtg aatttgccca gctttttgcc gattacgaaa ttataccgcc acttcgccag
    11101 ttgacacggc gcacggtgct gctcacgcct gacgagtcag ccagtaatag tttgaatcgc
    11161 tgggaaggta aatctgctac cgttgggcaa cttatgggaa tgcgatacaa aggctgggag
    11221 tcaggctatg aggacgcatt tgtctatgac ctgggtgagt accggctggt ccttaagttt
    11281 tcacccggtt ttaaccacta caatgttgat agcaaagcgc taatgagctt ccgttctctt
    11341 cgagtgtaca gtgacaataa atccgtcact tttgccgaac ttgatgtgtt tgatttgagt
    11401 gaggcgttaa gcgcacctga cgtcattttc cattaaaaca cagcgggcag tgatgcaact
    11461 gcccgttatc aacacgactt accagcgggg aaagcgatga tcgtgcaaaa agagctggtt
    11521 gctatttacg attatgaggt ccctgtacct gaagatccgt tttccttcag acttgagatc
    11581 cataaatgct ctgaattatt tacaggttcc gtctatcgac tggagcgatt ccggctacgt
    11641 ccaacatttc atcaacgtga tcgagaagat gctgacccgc taataaatga tgcgttgatt
    11701 tatataagag atgagtgtat tgatgagcgg aaattacgag gtgaatcacc tgaaactgta
    11761 atagcaattt ttaatcgtga actacagaat atattcaacc aactaataga atattagttt
    11821 attaaagggg gaagcggttt tcccccttag ttgcgtttca atttgcccat tacttcaaca
    11881 aatcaatcaa ccgtaacaga gacactaata gctccagtac ggcaatgagc acggttaact
    11941 tttgtgtcag cgtgtccatc cgctctcctt attaaggagc gcaacaccag caccttaccc
    12001 ttacactgcc tgtcgatact ggtttacgtt tgttgagtcg ccatgttgca ctccggatca
    12061 gagcactacc tgcggcacca cccgtccggt agccaggact tgaaatgggt tcgatccggc
    12121 gcaacttccc aacagtcagg attataaatc tcattatctt aattgcaaca ctgtccagag
    12181 gtggttataa ttcgttcgtt gagatgccat gttgcactat cagtaactta acatcaaagt
    12241 agtaaataaa agaacctgcc tcaccagcag gtttttttat tttctgcgat ctactttcca
    12301 gatatttttt gccaatagcc caccataaat attcacatcc cggtcaaatg gtgcgttgaa
    12361 tataaa
//
LOCUS       DAZGXZ010000141        12149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000141 DAZGXZ010000000
VERSION     DAZGXZ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.141"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            170..646
                     /locus_tag="ACHKJF_004277"
     CDS             170..646
                     /locus_tag="ACHKJF_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0951532.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            643..2766
                     /locus_tag="ACHKJF_004278"
     CDS             643..2766
                     /locus_tag="ACHKJF_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0951533.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQTARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEESLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            2763..2975
                     /locus_tag="ACHKJF_004279"
     CDS             2763..2975
                     /locus_tag="ACHKJF_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951534.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            2975..4477
                     /locus_tag="ACHKJF_004280"
     CDS             2975..4477
                     /locus_tag="ACHKJF_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0951535.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVAMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            4467..6446
                     /locus_tag="ACHKJF_004281"
     CDS             4467..6446
                     /locus_tag="ACHKJF_004281"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP0951536.1"
                     /translation="MKNKSWFRMQAGGPGDADIYIYDEIGFWGVTAKQFVSELNALGD
                     ITHINLHINSPGGDVFEGIAIFNSLKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            6533..6859
                     /locus_tag="ACHKJF_004282"
     CDS             6533..6859
                     /locus_tag="ACHKJF_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP0951537.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            6852..7133
                     /locus_tag="ACHKJF_004283"
     CDS             6852..7133
                     /locus_tag="ACHKJF_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP0951538.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            7136..7759
                     /locus_tag="ACHKJF_004284"
     CDS             7136..7759
                     /locus_tag="ACHKJF_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951539.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMTAMAHQLRLVLKRK"
     gene            7772..8170
                     /gene="gpU"
                     /locus_tag="ACHKJF_004285"
     CDS             7772..8170
                     /gene="gpU"
                     /locus_tag="ACHKJF_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP0951540.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            8178..8927
                     /locus_tag="ACHKJF_004286"
     CDS             8178..8927
                     /locus_tag="ACHKJF_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951541.1"
                     /translation="MATPNPLEPVKGSGTTLWVYTGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKILFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEITVTDVAAG"
     gene            8947..9378
                     /locus_tag="ACHKJF_004287"
     CDS             8947..9378
                     /locus_tag="ACHKJF_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0951542.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            9405..9809
                     /locus_tag="ACHKJF_004288"
     CDS             9405..9809
                     /locus_tag="ACHKJF_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0951543.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRREEAQTERPDEDDMLMRKAAGLSGGVRFGADGKEI
                     VTVSDDMRSSTEDEAMLMMVSEGIAGGVRYGG"
     gene            9799..>12149
                     /locus_tag="ACHKJF_004289"
     CDS             9799..>12149
                     /locus_tag="ACHKJF_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0951544.1"
                     /translation="MAGNFADLTAVLTLDSTRFSEEAARVKKELGETSDLADLMAGRV
                     SQSFKKQAAAVEQGLSRQALAAQKAGMSVGQYKAAMRMLPMQFTDVATQLAGGQNPWL
                     ILLQQGGQVKDSFGGMIPMFRGLAGAISLPMVGVTSLAVATGALAYAWYQGDSTLSAF
                     NKTLVLSGNQSGLTAERMLTLSRAGQAAGLTFNQAGESLAALVSAGVRGGEQFDAINQ
                     SVARFASASGVEVDKVAEAFGKLTTDPTSGLIAMARQFRNVTAEQIAYVAQLQRSGDE
                     AGALQAANEAATKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQE
                     MLIKAEAAFKKADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQD
                     KNAQQQSDTEASRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTL
                     MAAAKKDYEATLKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQ
                     QRRDLWKAESQFAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERL
                     NALAQQADKFAQQQRAKRAAMDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMS
                     EQKKTWAAEDQLRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLT
                     GSEQNWRSFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAFGGGASASSGTAIEAAAA
                     NFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYR"
BASE COUNT         2825 a         2903 c         3863 g         2558 t
ORIGIN      
        1 tgattttatc gtcatcatca tcatgatggt aacttgttgt ttttaatgct gttagtatta
       61 aaaagatgat gattgtggtt gttttttgtt cgacatcttt atatggcggc atttctttac
      121 aaaaaaaaca gagtcacttc tgttctgcgg tttatgtgga gggatgtaaa tggacggcga
      181 gctgaaaaat atgaagttaa atattaatca actggcagcc ctttcaggtc tgcaccggca
      241 gactgttgcc gccagaatgg cggatgttcc tcttgcacca ggcagtaatg aaaagaaaaa
      301 actgtatctc ctgacggatt tgataacttc gttgctggaa aaaccaccat cttccgaaga
      361 tgaggatatg gatcctcacg ctcgtaaggc atggtatcag tccgagcgcg agcgtcttaa
      421 atttcagcat gaaactgttc agcttgtgcc agtcagtgat gtcaggcggt ccttttctgt
      481 cgtggtgaaa gcgatagttc aggtactgga aacctggcct gaccggctgg agagggacag
      541 ggggtggact gcatcacaac tgaatgaagt acagattgtg gttgatgaga tccgcgacac
      601 actggaaaag gcagtcattg actgttgtga tgaggccgat atgtgaatca ggtgaacgag
      661 agccatagcc gcgcatccga tatctggcgc gaagtggcct cgctgtttcg cccacctggc
      721 cggttaccag tagcggaagc catcaggcgt tatatgcggg taccacgggg agccaatact
      781 tccggtccgt gggagtcatc gctgacgccc tatatgatag accccattaa tacattatca
      841 gcccgtgaat atgacgcggt ggtgtttgtc ggaccggcgc gaaccgggaa aaccgaaggg
      901 ctgattgatg gctggattgt gtacggcatc atctgtgatc cggcggatat gctggtggtg
      961 cagatgaccg agacgaaggc gcgtgagcat tccagaacgc gtctttccag gacgtttcgc
     1021 cacagtccgg aggtcagcaa gcgcctcagt ccttcccgta atgacaacaa cgtccacgat
     1081 aaaatgtttc ttgacggctc attcctgaaa attggctggc cgtcgatcac cgtattttcc
     1141 tcctcggatt accgtcgtgt ggcgctgacg gattatgacc gttttcctga gaatgtcgac
     1201 ggtgaagggg atgccttcac gctggcctca aagcgtacca ccacctttat gtcctcgggg
     1261 atgaccctgg tcgagagttc accggggcgg gatatcactg acaccaaatg gcgttgtggt
     1321 ggagcacatg aggcaccgcc aacaacgggg atcctgtcac tgtataaccg gggagaccgc
     1381 cgccggtggt actggccgtg tccgcactgc ggggaatatt ttcagccggt gatggataac
     1441 atgaccggtt accggaataa ccctgatttt gtggctgccg ggcagacggc ccgcctgatg
     1501 tgtccgcact gccgcggcct gattgctccg gaacagaagc gtgaactgaa taaccagggg
     1561 atctggctgc gggaaggcga acgggcagcg gcggatggca gtatcaccgg aaccccccga
     1621 aattccagaa tagcaagttt ctggatggag gggccggctg cggcgtttca gacctgggaa
     1681 caactgattt ttaagctgct ggcggcagaa gaagagtatg agcgaaccgg cagtgaagag
     1741 agcctgaaag cggtggtgaa caccgatatc ggacgaccct atctgccccg ttcagccacg
     1801 gaacagcgta aaagtgaact gcttgaacag cgtgccgagc cgtttcccag gcgatctgtg
     1861 ccggatggtg tgcgttttat tgaggcaacg gttgacgtac agggcggtaa aaatcgccgt
     1921 tttgttgtgc agatcaccgg atacggagag cagggggaac gctggattgt tgatcgctac
     1981 aacatccggc attcactgcg ctgcagtccc aacggtgaaa gtctgccggt tgatccggcg
     2041 gcatatccgg aggactggga tttgttgctg acggatgtgt tccataaaac atggccgctg
     2101 gcttctgatc cggatgtgcg catgcgtctg atggccatgg cggtggatac gggaggggaa
     2161 gccggggtga cagataacgc ctatcgtttc tggcgtcgtt gccggagtga cggactgggc
     2221 aacagggtgt ttctgttcaa gggggatggt cttcgccgtg acaggctgat taaccgtacc
     2281 ttcccggata ataccggcag aagtgcccgc cgtgccagag ccagtggcga tgtcgcgctg
     2341 tggctggttc agacggatgc gtttaaggac cgtgtaaata atgccctgtg gcgtgacaca
     2401 ccagggccga attatatcca ctttcccgac tggctggggc ggtggtttta cgatgagctg
     2461 acctatgaag agcgcggcag tgacggaaaa tggcgaaaac cgggcagggg tgctaacgag
     2521 gcgtttgacc tgctggttta tgcggatgcg cttgctgttc tgcatggtta tgaaaagatc
     2581 cgctggccct ccgcaccgga ctgggcacag cgggaaacgt ggctcgtctt cccgcaggag
     2641 cgttctggtg aaacggtatc cccggaactg acggccgggg cagaaaaacg ccgtcgccgg
     2701 aagaaaaaac tgcggacgga gcgtgcggaa gataatccat ggataacatc aggaggctgg
     2761 ttgtgagcac agaagaagcc agagaaatga ttcagcggta ccgtgaagcg gaaatggccg
     2821 tactggaggg gaagtctgtc accttcaacg ggcagcaact gacgctggaa agcctttctc
     2881 agatccgcgc cggacgtcag gagtgggaac gcaggcttgc cgcgatggtg agccgcaggc
     2941 ggggaaaacc gggatttaaa ctggcgaggt tttaatggca attattgatg atgtgatagg
     3001 cgtgttttcc cccggatgga aagcagccag actgcgttca agggcgttaa tcatggccta
     3061 tgaggcggtg aaaccgaccc ggacacataa agcccggcgg gaaaatcgct ctgctgatca
     3121 gctcagtaaa tacggtgcgg tttccctgcg ggagcaggcc cgttttctgg atatcaatca
     3181 tgacctggtg attggtgtgt ttgacaagct ggaagagcgg gtgattggtg ccaggggaat
     3241 tattgtggag cctcagccat tacgaaaaaa cggggaaatg gcggcagagc tggctgcgga
     3301 tatccgccgg ttgtgggctg aatggtccgt gagtccggat gtgacagggc agtatacccg
     3361 tcccgtgctt gaacgtttac tgctgcggac ctggctgcgg gatggtgaag tgtttgcgca
     3421 gatggtcagt ggtgcgggaa acggtctgga acggacggcg ggagtgccat tctggcttga
     3481 ggcgatggag ccggattttg ttcccatgcg cactgatgaa tccgccggac tgaatcaggg
     3541 ggtttttctt gatgagtggg gaagaccgaa aaaatatctg gtttataaaa attatccggt
     3601 cagcggccgg cagagtgata cgaaagaaat cgctgccgga aaaatgatcc acctgaagtt
     3661 cactcgtcgt ctgcatcaga cgcgaggctc atccatgtta tcgggggtgc tgatgcggat
     3721 cagtgccctt aaagagtatg aggatgcgga actgacagcg gcgcgtattg ctgcggcgct
     3781 gggactgtat atccgtaaag gtgacggaca ggactatgaa gatccgggga tcaaagaaac
     3841 cgagcgggaa gtccatatca ccccgggtat tatttatgac gatttgcgca agggcgagga
     3901 tatcggcatg gtcaaatctg accgtcccaa tcccaacctt gaaactttcc gcaacggcca
     3961 gttgcgtgca gtggcagctg gcagtcgtct gagtttttcc agtgcggcgc gtaactataa
     4021 cggcacctac agcgcccagc ggcaggagct ggtcgagtcc acggatggtt acctgatcct
     4081 gcaggactgt tttattggtg cggtaacccg cccggtgtac cggacatggc tgaatatggt
     4141 ggttgcggca ggtctgctga aaattccggc ggatgtggcg atgaaaacgc tatataacgc
     4201 gacgtattcc ggtccggtga tgccgtggat cgacccggtt aaggaagctg aagcctggag
     4261 aattcagatc cggggtggtg cagcgacaga atctgactgg gtgcgtgccg gcgggcgcaa
     4321 tccggatgag gtcaaacgtc gccgcaaggc tgaaattgat gaaaacagca gactggggct
     4381 ggtctttgat actgaccccg tcaacgacaa aggaggcaac agtgccggaa ctgaacgaca
     4441 gtatcagcgt gacaccgaaa gccagcatga agaataaatc ctggttcagg atgcaggctg
     4501 gggggccggg tgacgcggat atttatattt atgacgagat tggtttctgg ggagttaccg
     4561 cgaagcagtt tgtcagcgaa ctgaatgctc tgggtgatat cacccacatt aatctccaca
     4621 tcaactcacc gggtggcgat gtctttgaag gcatcgccat ttttaattcc ctgaaaaatc
     4681 agggggcgac cattaccgtg tatgtggatg gcgttgccgc ctcgatggca tctgtgattg
     4741 cgatggccgg tgatacggtc attatgccgg aaaatgcctt catgatgatc cataagccat
     4801 ggggattcag tggcggggat gctgaggata tgcgcagtta tgctgatttg ctggataaag
     4861 tcgaatcggt actgttgcca gcctatgcgc agaaaaccgg aaaaaccacc gatgaaattg
     4921 ccgccatgct ggcggatgaa acctggatgt ccggtgccga atgtctggca cacggatttg
     4981 ctgaccaggt gacacccgct gttgaggcaa tggcatgtat tcagtcaaaa cgtacagagg
     5041 aatttaaaaa gatgccggaa tccatccgaa acatgattac tccgccacgc aacagtgccc
     5101 cgcgtgatac cacagtgaca atccctgcac cggcggtaac agaaccatca ccggtaccag
     5161 cagtgtctga tgaggcgacc attcgcgccc gcgttatggc tgagcagaaa gcacgcatgt
     5221 caggcattaa cgatctgttt gccatgttcg gcggtcgcta tcagacgctt caggcacagt
     5281 gcgtggctga tcctgactgt tcgctggaaa tggcccgtga acgtctgctg aatgaaatgg
     5341 gcaaggagtc ctcgccgacc aacaaaaaca caccggccca tatttatgcc ggaaacggca
     5401 attttgtggg ggacgggatc cgccaggcga tgctggcccg tgccggattt gaaaatgtcg
     5461 agaaggataa cgcctataac gggatgaccc tgcgtgaatg ggctcgcatg tcactgacgg
     5521 agcgcggtat tggggtggcc agttataacc ccatgcagat ggtcgggctg gcgctgacgc
     5581 acagcacctc tgattttggc aatattctgc tggatgtgtc gaacaagggg ctgatccagg
     5641 gctgggagga atcagaagaa accttccaga agtggacccg taagggacgc ctgtcagact
     5701 tcaaaacagc gtatcgcgtg gggatgggcg gttttggttc tctgcgccag gttcgtgagg
     5761 gggcggagta taaatacatc accacctcag atcgcaagga gaccattgca ctggccactt
     5821 acggggagat tttctccatc acccgccagg ccattatcaa tgatgatctg aatatgctgg
     5881 tggacgtgcc gatgaagatg gggcgtgcgg cgaaggcaac gattggtgac ctggtctaca
     5941 aggtgctgac ggataacccg aaactgtctg acggtaaggc gctgttccat gccgatcaca
     6001 aaaatattgc caccgggggg atctccgttt ccggactgga tgcggcccgt cagatgatgc
     6061 gcctgcagaa agaaggcgat cgcgccctga atatccgtcc ggcctttatg ctggtaccgg
     6121 tggcactgga gacggtggcg aaccagacca tcaaatcggc cagtgtgaaa ggggcggatg
     6181 caaacgccgg tgtcattaac cccatccaga actttgctga ggtgattgca gaagcgcgtc
     6241 ttgatgcggc agatccgaaa acctggtatc tggcggcggc acagggcact gacaccattg
     6301 aagtggcctg gctggatggt gtggacacgc catacattga tcagcaggaa ggtttcacca
     6361 ctgacggtat tgccacaaaa atccgtattg atgccggagt ggcaccactt gactggcgcg
     6421 ggctggtgcg ttcgtcggtg gcctgataac cgcgttatca caatcactgc ccgaaagggc
     6481 ttttttatgc ctgaaaaaca gccccacagg ggctgtccgg agaaacagca ttatggcgaa
     6541 aaattttgta caggacggta ccaccattga actggtgaat gccggagatc agaccatcct
     6601 gagcggtgct gcggtggtgg tcggcagtat ggtggccgtg gccattaccg atattcctgc
     6661 cggtgaggcc ggtgacggtt ttgccgaagg cgtgttcctg ctgcccaaac agtctgctga
     6721 cgacattcag tccggcgcgg tggtttatct gaaggacggg gttgtgcagc tggctgcaga
     6781 cggtgcggtg gcagcgggag tagcctggga aaatgctcct gcaaacagcg ccactgtggc
     6841 ggtaaaaatc aatgtctgat ctgtttacgc gaatgtgttg ccggatggac gtggcgaccg
     6901 ttcggatgat gggcaaacag gcggagatta acggcgtcgt gtacgacgtg atgccggagg
     6961 aagagtccgc ggagatgggg gcgctttcgg gcagccagtt gtcactggtg gtgttttcag
     7021 cccggtaccg tccggcccgt catgatgttg ttgtgtttgc ggggcgcaca ctgacggtga
     7081 cccgttatga cacgtacaac ggtaaacccc ggatttttgt cgaacaggaa tgagtatggc
     7141 aataaaaggt ctggcgcagg ccatgaaaaa tctggatgca attgatcgcc gtgccgttcc
     7201 ccgggcctct gccacgacac tgaaccgcgt ggcgggggcc attattgcga aaacggcctc
     7261 ttcagttgcc agggagctgg ccgttccccg tcgtcttatc cgtgcccgca tccggttaag
     7321 tccggcacga ccggataagg tttacgcaaa ggtttacatc aataccggca acctgcccgc
     7381 catcaaactg ggggaggccc gcgttcgact ttcccgcaga aaacggagaa agaaaggaca
     7441 gcgtgcggcc ctgaaagggg gcggcagtgt gctgattgtg gggaaaagac ggatcccgga
     7501 cgcctttatc acccggctgg ctaacggacg ctggcatgtg atgcagcgta tgccgtgggc
     7561 atcatcgtcc accggcgcgg acagcaaagg gaggccgaaa cgccaccgtc tgccgatcga
     7621 agtggtgaag attccgactg ccggaccgct ggcagaaacc tttgaacgtg aacgggaccg
     7681 gatgtaccgg gaaaaattac cggcgcagat gatgacggcc atggcgcatc agttacgcct
     7741 ggtgctgaaa cgaaaatgac agggagggtg tatgaaacac cgtgaaatac gggcggcagt
     7801 tctgtctgcc ctgaaagaaa atatttctga gagggtgagc tggtttgacg gtcgtccggt
     7861 ttttattgat gaacaggaac tgcctgctgt tgctgtttac ctgacagatg cgtctgctgc
     7921 tgacgagttc gttgatgagg gaacctggga ggcgacactg catattgagg tttttctcag
     7981 ggcaaaagaa ccggactcgg cactggatat gtggatggaa gaaaaaattc ttcctgcgct
     8041 ggaggcagtt cccgggctca gtgcattact gctgaagatg aatcttcagg ggtatgacta
     8101 ccgccgggat gatgagttta tgatgtgggg atcggcagat ctcctgtgga aaattaccta
     8161 cgagatgtga ggacgatatg gcaacaccaa atcctcttga gccggtgaaa ggttccggta
     8221 ccacactgtg ggtgtacacc ggcactggtg atgcttatgc aaacccgttg tcagacgatg
     8281 actggcagcg actggcgaag gtgaaggatc tgacccccgg cgagatgacg gcggaatcct
     8341 acgatgataa ctatctggat gatgaggatg ctgactgggt atccaccggg caggggcaga
     8401 aatctgccgg tgacaccagt tttacgctgg cctggaagcc gggtgagaaa gggcagcgcg
     8461 atttgattgc ctggtttgac agcagtgaga cccgggccta caaaattctc ttcccgaacg
     8521 gtacggtgga tgtgttccgt ggctgggtga gcgccattgg taaagcggtg accgccaaag
     8581 aggtgatcac ccgtacggta aaaatcacca atatcggtcg tccgtcgctg gcggaagatc
     8641 agggggacat cacaccggtc accggtatta ccgtgacgcc accaacgggc aatgtggcaa
     8701 aaggtcagaa tatcaccctg accgtggccg ttcagccgga aggcgcgacg gataaaacgt
     8761 tccgcgcaac ctcagcgaat cagaatttcg ccaccattac cgtgaagggg aacacgatca
     8821 cggtgaaagg tgttgcggca ggtaaagcac agatccctgt ggtcaccggc aatggtgagt
     8881 ttgcggcggt ggcggaaatt accgtcacgg atgttgcagc gggttaagct gagggagtga
     8941 taaagcatgt ttctgaaaac agaacaattt gaatataacg gtgtgtctgt cacgctttct
     9001 gagctgtctg cgctgcagcg tattgagcat cttgccctcc tgaaacggcg ggcagaagag
     9061 gctgaagcca gcggtaacct gcaggtgagc gtggaggacc ttgtcagaac cggggcgttt
     9121 ctggtggcga tgtccctgtg gcataaccat ccacagaaaa cggcgtcacc gtcaatgaat
     9181 gaggccgtga tgaagataga gcaggaagtg ctcaccacct ggcctgccga tgccattgcc
     9241 cgggcggaag acgttgtgtt gtgcctgtcc gggatgatcg aagctgttcg tccggatact
     9301 gatattactg aagtggcgaa aaataacgcg ctgactgatg atgatttttc tgcgggaaag
     9361 tcttcgacgg cgagctgaac tttgccctca gactggcgcg tgagatgggg agacccgact
     9421 ggcgcgccat gcttgccggg atgacatcca ccgaatatgc cgactggcac cgtttttacc
     9481 gcacgcatta ttttcacgat actcagctgg atatgcattt ttccgggctg acgtacgccg
     9541 tactcagcct gtttttttgc gatccggata tgcatccctc ggatttcagt ctgcttgtcc
     9601 cccggcgtga ggaagcgcag acggagaggc cggatgagga cgatatgctg atgcggaaag
     9661 cggcaggact ttctggtggt gtccgctttg gggctgacgg gaaggaaatc gttacggtca
     9721 gtgatgacat gcggagcagc acagaggatg aagccatgct gatgatggtg tctgagggaa
     9781 ttgcaggagg tgtacgctat ggcgggtaat tttgccgatc tgacagctgt tcttacactg
     9841 gattcaaccc gtttttctga agaggctgca cgggtaaaga aagaactggg tgaaaccagt
     9901 gaccttgcgg atttgatggc cgggcgtgtc agccagtctt ttaagaaaca ggccgctgcc
     9961 gttgaacagg ggctgagccg tcaggccctg gctgcacaaa aagccgggat gtccgtcggg
    10021 cagtataaag cggccatgcg tatgctaccc atgcagttca ccgacgtggc cacgcagctt
    10081 gccggtggtc agaatccctg gctcatcctg ctgcaacagg gcggtcaggt gaaggactca
    10141 ttcggcggga tgatccccat gttccggggg cttgccggtg cgatcagcct gccgatggtc
    10201 ggggtcacct cgctggcggt ggcgaccggt gcgctggcgt acgcctggta ccagggggat
    10261 tccacgcttt cagcgtttaa taaaaccctg gttctttccg gtaatcagtc aggactgacg
    10321 gcagagcgca tgctgacgct ctccagagcc gggcaggcgg cagggctgac gtttaaccag
    10381 gcgggagagt cactggcagc cctggtcagt gccggtgtgc gtggtggtga acagtttgat
    10441 gccattaacc agagtgtggc gcgttttgcg tctgcctccg gtgtggaggt ggacaaggtt
    10501 gcagaggctt tcggaaaact gaccaccgac ccgacgtcgg ggctgattgc gatggcacgc
    10561 cagttccgta acgtgacggc agagcagatt gcgtatgttg ctcagttgca gcgttccggc
    10621 gatgaagccg gggcattgca ggcggcgaac gaggccgcca cgaaagggtt tgatgaccag
    10681 acccgccgcc tgaaagagaa catgggcacg ctggagacct gggcagacag gactgcgcgg
    10741 gcattcaaat ccatgtggga tgcggtgctg gatattggtc gtcctgatac cgcgcaggag
    10801 atgctgatta aggcagaggc tgcgtttaag aaagcagacg acatctggaa tctgcgcaag
    10861 gatgattatt ttgttaacga tgaagcgcgg gcgcgttact gggatgatcg tgaaaaggcc
    10921 cgtcttgcgc ttgaagccgc ccgaaagaag gctgagcagc agactcaaca ggacaaaaat
    10981 gcgcagcagc agagcgatac cgaagcgtca cggctgaaat ataccgaaga ggcgcagaag
    11041 gcttacgaac ggctgcagac gccgctggag aaatataccg cccgtcagga agaactgaac
    11101 aaggcactga aagacgggaa aatcctgcag gcggattaca acacgctgat ggcggcggcg
    11161 aaaaaggatt atgaagcgac gctgaaaaag ccgaaacagt ccggcgtgaa ggtgtctgcg
    11221 ggcgatcgtc aggaagacag tgctcatgct gccctgctga cgcttcaggc agaactccgg
    11281 acgctggaga agcatgccgg agcaaatgag aaaatcagcc agcagcgccg ggatttgtgg
    11341 aaggcggaga gtcagttcgc ggtactggag gaggcggcgc aacgtcgcca gctgtctgca
    11401 caggagaaat ccctgctggc gcataaagat gagacgctgg agtacaaacg ccagctggct
    11461 gcacttggcg acaaggttac gtatcaggag cgcctgaacg cgctggcgca gcaggcggat
    11521 aaattcgcac agcagcaacg ggcaaaacgg gccgccatgg atgcgaaaag ccgggggctg
    11581 actgaccggc aggcagaacg ggaagccacg gaacagcgcc tgaaggaaca gtatggcgat
    11641 aatccgctgg cgctgaataa cgtcatgtca gagcagaaaa agacctgggc ggctgaagac
    11701 cagcttcgcg ggaactggat ggcaggcctg aagtccggct ggagtgagtg ggaagagagc
    11761 gccacggaca gtatgtcgca ggtaaaaagt gcagccacgc agacctttga tggtattgca
    11821 cagaatatgg cggcgatgct gaccggcagt gagcagaact ggcgcagctt cacccgttcc
    11881 gtgctgtcca tgatgacaga aattctgctt aagcaggcaa tggtggggat tgtcgggagt
    11941 atcggcagcg ccattggcgg tgctttcggt ggtggtgcat ctgcttcctc ggggacggcc
    12001 attgaggctg cggcggcgaa cttccatttc gcgaccggag gatttacggg gacgggcggc
    12061 aaatatgagc ctgcggggat agttcaccgc ggggagtttg tcttcacgaa ggaggcaacc
    12121 agccggatag gtgtggggaa tctttaccg
//
LOCUS       DAZGXZ010000142        12060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000142 DAZGXZ010000000
VERSION     DAZGXZ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.142"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            258..2495
                     /gene="nrfB"
                     /locus_tag="ACHKJF_004290"
     CDS             258..2495
                     /gene="nrfB"
                     /locus_tag="ACHKJF_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP0951545.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2482..5454
                     /gene="nfrA"
                     /locus_tag="ACHKJF_004291"
     CDS             2482..5454
                     /gene="nfrA"
                     /locus_tag="ACHKJF_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP0951546.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDASAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNTQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5455..6345
                     /gene="ybcH"
                     /locus_tag="ACHKJF_004292"
     CDS             5455..6345
                     /gene="ybcH"
                     /locus_tag="ACHKJF_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP0951547.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            6528..7289
                     /gene="envY"
                     /locus_tag="ACHKJF_004293"
     CDS             6528..7289
                     /gene="envY"
                     /locus_tag="ACHKJF_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP0951548.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            7804..8757
                     /gene="ompT"
                     /locus_tag="ACHKJF_004294"
     CDS             7804..8757
                     /gene="ompT"
                     /locus_tag="ACHKJF_004294"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP0951549.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            8944..10428
                     /locus_tag="ACHKJF_004295"
     CDS             8944..10428
                     /locus_tag="ACHKJF_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0951550.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
     gene            complement(10611..10823)
                     /locus_tag="ACHKJF_004296"
                     /pseudo
     CDS             complement(10611..10823)
                     /locus_tag="ACHKJF_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            10973..11641
                     /locus_tag="ACHKJF_004297"
     CDS             10973..11641
                     /locus_tag="ACHKJF_004297"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0951551.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(11586..11723)
                     /locus_tag="ACHKJF_004298"
     CDS             complement(11586..11723)
                     /locus_tag="ACHKJF_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFP0951552.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(11696..>12060)
                     /locus_tag="ACHKJF_004299"
     CDS             complement(11696..>12060)
                     /locus_tag="ACHKJF_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0951553.1"
                     /translation="TVYDVASGDALFISELGPLPENVTWLSPEGEFQKWNGTAWVKDA
                     EAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEETSLLEAWKKYRVLLNR
                     VDTSTAPDIEWPTNPVRE"
BASE COUNT         3262 a         2984 c         2825 g         2989 t
ORIGIN      
        1 ctgctcacct cctccaagca cttccactac atgatgacct gatcggtgcc accactcctc
       61 ttcactatga cgttaacctc ccctccttga caccacgctt ttgtctacat tctacgcaaa
      121 cggcaacttt tataaccagt gtaaaaataa cgtgccgcaa tagtcatacc tattaatggt
      181 aaaaagctgt cacaattcat aaaaaacctt aatatacgcc accctaaaca taaccagcgt
      241 taatgtaagg tttttgtgtg gactggcttc ttgatgtttt tgcaacctgg ctctacggct
      301 taaaagtaat cgcgataacg ttagcggtca tcatgttcat cagcgggctg gacgattttt
      361 ttattgatgt cgtctactgg gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct
      421 acccgcgaat gagttaccgc gaactgtata aaccagatga aaaaccgtta gcgattatgg
      481 ttccggcgtg gaatgaaacg ggcgtcatcg gcaatatggc cgagctggcg gcgaccacgc
      541 tcgactacga aaactatcat atctttgttg gcacctaccc caacgacccc gatactcaac
      601 gtgatgttga cgaagtgtgc gctcgctttc cgaacgtgca taaggtagtc tgcgcgcgtc
      661 ctggtcccac cagcaaagcc gactgtctga acaacgtgct ggacgccatc acccagtttg
      721 aacgtagcgc caatttcgct tttgctggtt ttattctgca tgacgccgaa gatgtgattt
      781 caccgatgga attgcgtctg ttcaactatc tggtcgagcg taaagatctg attcagatcc
      841 cggtgtatcc gttcgaacgc gaatggacgc acttcaccag catgacttac attgatgagt
      901 tttcagagct gcatggcaaa gatgttccgg tgcgtgaagc cctcgccgga caagtgccca
      961 gcgcaggcgt cggcacctgt ttcagccgcc gcgccgtgac cgctctgtta gctgacggtg
     1021 acggtattgc tttcgacgtg cagagtctga ctgaagatta cgacattggt ttccgcctga
     1081 aagagaaagg tatgacggaa atttttgtcc gttttccggt ggtggacgaa gccaaagaac
     1141 gcgagcagcg taaattttta cagcacgcac ggacgtcaaa catgatctgc gtgcgcgaat
     1201 atttccccga taccttttcg actgcagttc gacaaaaatc tcgctggatc atcggcattg
     1261 ttttccaggg ctttaaaacc cacaaatgga cctccagcct gacgctgaac tactttctct
     1321 ggcgcgaccg caaaggggca atcagtaact ttgtcagctt cctcgcgatg ctggtgatgc
     1381 tccagctttt gctgttgctg gcgtatgaaa gtttgtggcc cgatgcctgg catttccttt
     1441 ctatttttag cggcagcgca tggttaatga ccctgctgtg gctaaacttt ggcttgatgg
     1501 ttaaccgcat cgtgcagcgg gtgattttcg tcactggcta ctacggtctg acgcaggggc
     1561 tgttatctgt cctgcgtctt ttctggggca acctgattaa cttcatggcc aactggcgcg
     1621 cgctaaaaca ggtacttcaa cacggcgatc cacgtcgcgt cgcgtgggat aaaacaacgc
     1681 atgacttccc cagcgtgact ggcgataccc gctcgttgcg cccgttaggc caaattctgc
     1741 tggaaaatca ggtcatcact gaagaacaac tcgatacagc actgcgtaat cgcgtcgaag
     1801 gtctacgctt gggcggttca atgctgatgc aggggctgat tagcgccgag cagctggcac
     1861 aggcgctggc agagcaaaac ggcgtggcgt gggaatccat cgatgcctgg cagatcccgt
     1921 cctcgctgat tgccgaaatg ccggcctccg tagcgctgca ttatgcagta ctgccgctgc
     1981 gtctggaaaa tgatgagtta attgtcggca gtgaagatgg tattgacccg gtttcgctgg
     2041 cggccctgac gcgtaaagtc ggacgcaaag tgcgttacgt cattgttctg cggggacaaa
     2101 ttgtcacggg gttacgtcac tggtatgcac gccgacgcgg tcacgatccg cgggcaatgt
     2161 tgtacaatgc ggttcagcat cagtggctca cggaacagca ggccggtgaa atctggcggc
     2221 aatatgtgcc gcatcagttc ctgttcgccg aaatactgac cacgctcggt catattaatc
     2281 gttcagcaat taacgtgttg ttattgcgcc atgaacgcag ttctctgccg ctcggcaagt
     2341 ttttggtcac cgaaggcgtt atcagccagg aaacgttgga tcgcgtcctg acaattcaac
     2401 gcgaattaca agtttcgatg caatcactat tactcaaagc aggtttaaac acagaacagg
     2461 ttgcgcaact ggagtccgaa aatgaaggag aataacctta atcgcgtcat cggatggtct
     2521 ggtttactgc tgacgtcttt attgagtacc agcgcactcg cagacaatat cggcaccagc
     2581 gcagaagagc tggggctgag cgattatcgc cattttgtta tttatccccg tctcgataag
     2641 gcgctgaagg cacagaaaaa taacgacgaa gcaaccgcca tccgcgaatt tgaatatata
     2701 caccagcagg tgccggataa tattccgctg actttatacc ttgcggaagc ctatcgccat
     2761 tttggtcatg atgaccgggc gcggctgttg cttgaggatc aactgaaacg tcacccagga
     2821 gatgcccgac ttgagcgcag tctggcggct attccggttg aagtgaaaag cgttacgact
     2881 gttgaagaac tgcttgccca gcaaaaagcg tgcgacgctg cgccgaccct gcgttgtcgc
     2941 agtgaagtcg ggcagaatgc cctgcggctg gcacaattac ctgtcgccag agcgcaactg
     3001 aacgatgcga cgtttgctgc atcgccagaa ggaaaaacgc tgcgaaccga tctgctgcaa
     3061 cgggcaatct acctgaaaca atggtcccag gcagatacgc tatacaatga agcacgccag
     3121 cagaacacat taagcgcggc agaacgccgt cagtggtttg acgtgcttct tgccgggcag
     3181 ctggacgatc ggatcctggc actgcaatca caggggatct tcaccgatcc tcagtcatat
     3241 attacttacg cgaccgcgct ggcttatcgt ggcgaaaaag cacgcctcca gcattatctc
     3301 aatgaaaata agccgctgtt taccacggac gcacaagaga aaagttggct ctatctgtta
     3361 tctaaataca gcgccaaccc cgttcaggca ttggcgaatt atacggtgca gtttgccgat
     3421 aaccgccagt atgttgttgg cgcgacgcta ccggtgctgt taaaagaagg tcagtacgac
     3481 gcagcgcaaa aactgctcgc caccctcccc gccaatgaaa tgcttgagga gcgttatgct
     3541 gtcagcgtgg cgacccgtaa caaggctgaa tctctgcgtc tggcacgatt gctgtatcag
     3601 caagaaccgg caaatcttac ccgcctggat caactgacct ggcaactgat gcagaacgag
     3661 cagtcacgcg aagctgccga tttattgctg caacgctatc ctttccaggg cgatgcgcgt
     3721 gtcagccaga ctttaatggc gcgactggcg tctctgctgg aaagtcatcc ttacctggca
     3781 acgccggcga aggtggcgat tttatcgaaa cccttaccgc tggcggagca acgtcagtgg
     3841 caaagtcagt tgccgggtat tgcagataat tgcccggcaa tagttcgctt gctgggcgat
     3901 atgtcgcctt cctacgatgc ttccgcctgg aaccgtctgg caaagtgtta tcgggacacg
     3961 ctacccggtg tggcgctgta tgcatggctt caggccgaac aacgacaacc gaacgcctgg
     4021 caacatcgtg cggtagccta tcaggcgtat caggttgagg actacgccac cgcactggcg
     4081 gcctggcaga aaatcagtct tcacgacatg agcaatgagg atctgcttgc tgctgccaat
     4141 accgcccagg cggcaggaaa tggtgcggct cgcgatcgct ggcttcagca ggcagaacaa
     4201 cgtgggctgg gaaacaatgc cctctactgg tggctgcatg cgcaacgtta cattcctggt
     4261 caaccggaac tcgcactgaa cgatctcacg cgctcaatca atattgcgcc ttctgccaac
     4321 gcttacgttg cgcgggcgac aatttatcgc caacgtcata atgtcccggc ggcggtgagt
     4381 gatttgcgcg ccgctctgga actggaaccg aataatagca acacccaggc agcgctcggt
     4441 tacgccttgt gggatagcgg tgatatcgca cagtcgcggg aaatgctcga acaggcgcat
     4501 aaagggctac cggacgatcc ggcacttatc cgacaactgg cctacgtgaa ccagcgtctg
     4561 gatgacatgc ctgcgacgca gcactacgcc cggctggtga ttgatgacat tgataatcag
     4621 gcgctgataa ccccactgac cccagagcaa aatcagcaac gcttcaattt ccgccgtttg
     4681 catgaggagg tcggtcgccg ctggacgttc agtttcgatt cttccatcgg cttgcgttcc
     4741 ggcgcaatga gtaccgctaa caataatgtc ggcggcgcag cgccagggaa aagctatcgt
     4801 agctacggac aactggaagc cgagtaccgc atcggacgca atatgctgct ggaaggcgac
     4861 ctgctctcag tttatagccg cgtctttgcc gataccggag aaaacggggt gatgatgccg
     4921 gtgaaaaatc cgatgtccgg caccggtctg cgctggaagc cgctgcgcga tcagatcttt
     4981 ttcctcgccg tcgaacagca gttgccgctg aacggccaaa atggcgcatc cgataccatg
     5041 ctgcgcgcca gcgcctcatt ctttaatggc ggcaaataca gcgacgaatg gcacccgaac
     5101 ggttcaggct ggtttgccca aaacctgtac ctcgatgcgg cgcaatatat ccgccaggat
     5161 attcaggcgt ggacggcaga ttatcgcgtc agctggcatc agaaggtagc taacggacag
     5221 actattgagc cttacgctca cgttcaggac aacggctatc gtgataaagg cactcagggc
     5281 gcgcagcttg gcggagtcgg agtccgctgg aatatctgga ccggcgagac gcactacgac
     5341 gcctggccgc acaaagtcag tctcggcgtc gagtatcaac atacctttaa ggcgattaat
     5401 caacgtaacg gagagcgcaa caacgcgttt ctcaccattg gagtgcactg gtaaatgcgt
     5461 aagttcattt tcgtattgct gacactgctt ttggtcagcc ctttttcctt tgcgatgaaa
     5521 ggtattatct ggcaaccaca aaaccgagat agtcaggtta ccgataccca gtggcagggg
     5581 ctgatgagtc agttacgttt gcaaggcttc gataccctgg ttttgcaatg gacccgttac
     5641 ggcgatgcat tcacccagcc agaacagcgc gcgttattgt ttaagcgtgc cgcagctgcg
     5701 caacaggctg gcctgaagct gattgtcggg ctgaacgccg atccggaatt ttttatgcac
     5761 cagaaacagt cgtccgcagc gctggaaagc tatcttaatc gcctgctggc tgccgatctc
     5821 cagcaagcca gattatggag cgccgcgcct ggcgtaacgc cggatggctg gtacatcagc
     5881 gcggaaattg acgacctgaa ctggcgcagc gaagctgccc gtcagccttt gctaacatgg
     5941 ttaaacaacg cgcagcggct gattagcgat gtttcagcaa aaccggttta tatcagtagt
     6001 tttttcgccg gaaacatgtc gcccgatggc tatcgccaac tgctggaaca agttaaagca
     6061 accggcgtta atgtctgggt acaggatggc agcggcgtgg ataaacttac cgctgaacag
     6121 cgtgaacgtt atctacaggc cagcgccgat tgccaaagtc ccgcccctgc cagcggcatt
     6181 gtttatgaac tgtttgtcgc cggcaaaggc aaaaccttta cagcgaaacc gaaaccggat
     6241 gcagagatcg cctcgctgtt agcgaaacgt tcctcttgcg gtaaagacac tctctatttc
     6301 tctctgcgct atttgcccgt cgcgcacggc attctcgagt attaaatctc ctccaggtaa
     6361 gtcgggtacg acctggctta cctctttcgc tctcgtcaga attccatcaa gattaattgc
     6421 taaaaagcgt gctaaagaaa gaagatcgtt atcggcgaac gggccgatca ctcatgcagg
     6481 aagcatagat cctttgagcg gagtttctga tatctgagga gtgcgaaatg caattgagca
     6541 gcagtgaacc ttgcgtggtg atcctgaccg aaaaagaggt agaggtaagc gtcaataacc
     6601 atgctacgtt tacccttccg aaaaactacc tggccgcctt cgcgtgcaac aataacgtca
     6661 ttgaactctc aacgttaaat cacgtattaa tcacccacat caaccgtaac atcatcaacg
     6721 attatctgtt gtttttaaat aagaacttaa cctgtgtaaa gccctggtcg cggctggcaa
     6781 ccccggttat tgcttgtcat agccgtacac cggaagtgtt ccggctagcc gccaaccaca
     6841 gcaagcagca acccagcaaa ccctgcgaag cggagttgac gcgcgtattg ctttttaccg
     6901 tattgtctaa ctttcttgag caatcgcggt ttattgccct actgatgtat atcttacgca
     6961 gcagcgtccg cgacagcgtc tgccgcatta ttcaaagcga tattcagcat tactggaatc
     7021 tgcgaattgt cgccagttcg ctatgtttaa gccccagcct gctcaaaaag aaattaaaaa
     7081 acgaaaatac cagctatagc cagattgtca cagagtgtcg tatgcgttac gccgtacaga
     7141 tgttattgat ggataacaaa aatatcactc aggtggcgca attatgtggc tatagcagca
     7201 cgtcatactt tatctctgtt tttaaggcgt tttacggcct gacaccgttg aattatctcg
     7261 ccaaacagcg acaaaaagtg atgtggtgaa gggcaaagcg gaaacggata agacgggcat
     7321 aaatgaagaa gaaatggcgc gccctgcagg attcgaacct gcggcccacg acttagaagt
     7381 tcctagaacg acattttaag tcaacaactt accgcgccat ctctgcgctc acacgtccca
     7441 ctacctcaaa acatgtaaag ccttgcaagc cattgtgagg ccttatgtgt ctcagttttg
     7501 tccctctttt ttgtactaaa aaacatagca attgaggata aacctcatgc tattttcgct
     7561 tatatgcctc taaaggcatg gcgcttaaat agataaaaac accacaaaag cataaaaaaa
     7621 ccacacagta aaaccgcaat ataaaacaat aacagataat taaaccaaaa acagatagac
     7681 gcattgtgat aatcattcaa tactaaacaa aatataaaca gtggagcaat atgtaattga
     7741 ctcattaagt tagatataaa aaatacatat tcaatcatta aaacgattga atggagaact
     7801 tttatgcggg cgaaacttct gggaatagtc ctgacaaccc ctattgcgat cagctctttt
     7861 gcttctaccg agactatatc gtttactcct gacaacataa atgcggacat tagtcttgga
     7921 actctgagcg gaaaaacaaa agagcgcgtt tatctagctg aagaaggagg ccgaaaagtc
     7981 agtcaactcg actggaaatt caataacgct gcaattatta aaggtgcaat taattgggat
     8041 ttgatgcccc agatatctat tggggctgcg ggctggacta ctctcggcag ccgaggtggc
     8101 aatatggtcg atcaggactg gatggattcc agtaaccccg gaacctggac agatgaaagt
     8161 aggcaccctg atacacaact caattatgcc aacgaatttg atctgaatat caaaggctgg
     8221 ctcctcaacg aacccaatta ccgcctggga ctcatggccg gatatcagga aagccgttat
     8281 agctttacag ccagaggtgg ttcctatatc tacagttctg aggagggatt cagagatgat
     8341 atcggctcct tcccgaatgg agaaagagca atcggctaca aacaacgttt taaaatgccc
     8401 tacattggct tgactggaag ttatcgttat gaagattttg agctaggtgg cacatttaaa
     8461 tacagcggct gggtggaagc atctgataac gatgagcact atgacccagg aaaaagaatc
     8521 acttatcgca gtaaagtcaa agaccaaaat tactattctg ttgcagtcaa tgcaggttat
     8581 tacgtaacac ctaacgcaaa agtttatgtt gaaggcgcat ggaatcgggt tacgaataaa
     8641 aaaggtaata cttcacttta tgatcacaat gataacactt cagactacag caaaaatggt
     8701 gcaggcatag aaaactataa cttcatcact actgctggtc ttaagtacac cttttaacga
     8761 agttaaccag attttctccc cggtggtatt gtataacccc ggggaatttg tttacagtta
     8821 caagtagaca atatccacta attttgtaag catttaacct atgtcataac gtgtaatctc
     8881 ttaccgcaac acaagacctc aatatattca aatcttatct ttattccgat attcagagac
     8941 acaatgcgaa aaataattac tcatttcaaa gttgttttaa cgttacttct accagtaacc
     9001 gtatctgccc agcagataca gtggcaatcc tgtatggcca gtcaattcaa ccactggttt
     9061 ggtgaggaaa aaccgtctcc tgacttacta tgtggttatt tgtctgttcc attaaaatat
     9121 acagacacag gcggagatgc ttcttatgaa aaaaaatcac aagtcaaact agcgttgaca
     9181 aaattgccgg caaaaagcaa gcataaagga agtatcctga taataagtgg tggtcccggg
     9241 ttaccaggca taaatcctta tattaacttt gactggccag tcacaaatct tcgtgagtca
     9301 tgggatatta ttggatttga tcctcgaggc gtcggacagt ccactccgac aataaactgc
     9361 cggcaatcag atacagagac tcaggaaaac ataactgaaa agcaacaagt attaaataaa
     9421 attaatgcct gtatccataa taccggagcc gaagtcattc gccatatcgg ctctaacgag
     9481 gctgtatacg atattgatcg tattaggcaa gccttggggg ataaacaact gacagccgtg
     9541 gcgtattcgt atggaactca aattgcagcc ttatatgcag aacgttttcc ctacaacgta
     9601 agatctatcg ttcttgatgg agtcgtcgat atcgatgacc tggaggacaa cttcacatgg
     9661 caactcaaac aggcacagag ttatcaggaa acgtttgatc gctttgcatc ctggtgtgcg
     9721 cgtacaaaaa gttgcccgct ttcttcagac agagataagg caataactca gttccatgag
     9781 ctattatcaa aattacatca caaaccttta ttagacagta agggagaaaa tatatcttca
     9841 gatgaactca tatcattaac aacagacctt ctgctatggc gttcatcatg gccaaccctt
     9901 gcaactgcca tacgccagtt ctctcagggg attgtcagta atgaaattga aactgcgctc
     9961 agtgctccga tagcctcaga agagtcaagc gatgcttcgg gggtaatcct ctgtgtagat
    10021 cagggggatg agcaattaac accagaagag cgaaaatccc gaaaagacgc tcttgcgaat
    10081 gccttcccgg ctattaactt tgacaatgga cgttccgatt cacctgattt ttgtgaatta
    10141 tggccaatac atagcgacct gaacaaaact cgcctgaaaa atactgttct gccctctggt
    10201 ttactgtttg tagcacacaa atacgaccca acaacgccct ggattaatgc ccgtaagatg
    10261 gcagagaaat tttccagccc gttactaaca ataaatggtg atgggcatac attagctctc
    10321 accggagtta atttatgtgt agataaagca gttgtacatc acctgatcac tccacaaaaa
    10381 atagaaaata tatactgccc aggaaattct gaagcagaaa tacaataatt tcaaaatatt
    10441 ccgctatttg ctcccaatgc aaaacatata ttgcattggg agcatattca tatttttgtt
    10501 attcttgtat gaaatcgtct catgtacacg gtcctttttc gccgaaaact gatattactt
    10561 taatcaaaag ttgacgtgcc agattcgaag taaatttata aaatatcaaa tcaatataca
    10621 ttttgcacct tcgagatagt aatggctgtt actgtaacat gagttacaaa cagcttcata
    10681 cccagagcta ctgatgtatc aacacgattc aacaataccc gatatttatt ccatgcctcc
    10741 agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg cagtggcgca
    10801 atatactcac tgaattcctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc
    10861 tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa
    10921 ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata acatggtgaa
    10981 attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct
    11041 ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga
    11101 acaccttctt ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg
    11161 aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa
    11221 cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat
    11281 tagccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg attccatttc
    11341 catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg
    11401 caatgcggcg caggccttaa ctgacgatgg aactctatac ggagccacaa ttcttggtga
    11461 tggagttgtg cacaatagct tcggtcaaaa actgatgcgt atttacaatc agaaaggcat
    11521 cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga
    11581 gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata
    11641 gcatccaacc gcagcacgtt cttgcttaag acgtgctgcg gcataatccc aatgattact
    11701 ccctgacagg gttcgtaggc cactcaatat caggtgcagt tgatgtatca acacggttca
    11761 gcaacacccg atactttttc caggcttcca gcaatgaggt ttcttcctcc gttgcgattt
    11821 ccagatctac agcatcctga agtggcgcaa tatgctcact ggctacctgc atcaggctgt
    11881 tttttgtttc ttccgcctct cggatccgga acagtttttc tgcttctgca tctttcaccc
    11941 aggctgtgcc gttccacttc tgaaactccc cttccggcga taaccaggta acattttccg
    12001 gtaacggacc gagttcagaa ataaataacg cgtcccctga cgctacgtca taaaccgttt
//
LOCUS       DAZGXZ010000143        12055 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000143 DAZGXZ010000000
VERSION     DAZGXZ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.143"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..563
                     /locus_tag="ACHKJF_004300"
     CDS             30..563
                     /locus_tag="ACHKJF_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0951554.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            855..986
                     /locus_tag="ACHKJF_004301"
     CDS             855..986
                     /locus_tag="ACHKJF_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951555.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAIFLFLPWMMLSSSGMLG"
     gene            950..1104
                     /locus_tag="ACHKJF_004302"
                     /pseudo
     CDS             950..1104
                     /locus_tag="ACHKJF_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788411.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1169..1630
                     /locus_tag="ACHKJF_004303"
     CDS             1169..1630
                     /locus_tag="ACHKJF_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0951556.1"
                     /translation="MNRILYVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCSGT
                     VREAPTTSGVDNATAPRLADTVERDYFTLRERLMLMQKQLEGAQDYIRTQC"
     gene            1930..2736
                     /locus_tag="ACHKJF_004304"
     CDS             1930..2736
                     /locus_tag="ACHKJF_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0951557.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPSNDACSDDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVITPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            2717..4423
                     /locus_tag="ACHKJF_004305"
     CDS             2717..4423
                     /locus_tag="ACHKJF_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0951558.1"
                     /translation="MTFRKNKTRCDEPAEMTETEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRNMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQESTASGLLLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYISKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPM
                     IVYDIEPVTGAKTKAQSLREGNKNELQRTLMNYLLVWELPDPDEEYVCGADTAEGLEH
                     GDRSSLDVVKRSNGEQVAHWFGHLDAELFAHLISQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            4423..6567
                     /locus_tag="ACHKJF_004306"
     CDS             4423..6567
                     /locus_tag="ACHKJF_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0951559.1"
                     /translation="MKNEINTTAMKNDHGSTPRFSQRQLLSLCSDIDSQPLWRDAANK
                     ACAYYDGDQLAPEVIQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLIVMSDDPND
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDAPQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            6725..7732
                     /locus_tag="ACHKJF_004307"
     CDS             6725..7732
                     /locus_tag="ACHKJF_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951560.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEQEQAVPASPSDLGTTASVGIGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            7756..8970
                     /locus_tag="ACHKJF_004308"
     CDS             7756..8970
                     /locus_tag="ACHKJF_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP0951561.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARMLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            9026..9415
                     /locus_tag="ACHKJF_004309"
     CDS             9026..9415
                     /locus_tag="ACHKJF_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951562.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHALIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            9465..9926
                     /locus_tag="ACHKJF_004310"
     CDS             9465..9926
                     /locus_tag="ACHKJF_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951563.1"
                     /translation="MSEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            9910..10473
                     /locus_tag="ACHKJF_004311"
     CDS             9910..10473
                     /locus_tag="ACHKJF_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951564.1"
                     /translation="MAELSDFLPYVRRQISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPSRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            10473..11123
                     /locus_tag="ACHKJF_004312"
     CDS             10473..11123
                     /locus_tag="ACHKJF_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP0951565.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLSCVPGARQTLPDGAIQLLDVICLSDGSAIKPVSREVLDAQYPDWHMLRG
                     KPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            11120..>12055
                     /locus_tag="ACHKJF_004313"
     CDS             11120..>12055
                     /locus_tag="ACHKJF_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFP0951566.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPK
                     GERGDVGAQGAVGPAGPRGEKGEQGKRGPQGIPGLK"
BASE COUNT         3089 a         2789 c         3463 g         2714 t
ORIGIN      
        1 gggacagcgg ctgaatattt aatatatcca tgaacgcaaa aatcaaatac ggcctgtcag
       61 ctgctgttct ggcgctgatt gccgcagggg cttcggcgcc tgaaatcctc gaccagtttc
      121 tggatgaaaa agaaggtaac cacaccacgg cataccgtga tggtgcgggt atctggacca
      181 tctgccgtgg agccacccgg gtggatggta agcctgtgat tcctggcatg aagctgtcga
      241 aggggaaatg cgaccgggtt aacgccattg agcgtgataa ggcgctggca tgggtggaga
      301 aaaacatcaa agtgccactg actgaacccc agaaagcggg aattgcgtca ttctgtcctt
      361 acaacattgg ccccggtaag tgtttcccgt cgacgtttta taaacgaatt aatgcaggcg
      421 atcgaaaagg tgcctgcgaa gcgattcgct ggtggattaa ggacggtggc agagactgtc
      481 gtatccgctc aaataactgt tatggtcagg tatcccgtcg cgaccaggag agtgcgctgg
      541 cgtgctgggg aatcgacaga taagcagaat attttgctga aaaatgacgt tggtcaacgc
      601 gggtggataa cacgaaatcc tgcgaactgg caaaacctaa gtgaataaaa gtaaaaaccc
      661 cgtttttggc agcaagcggg gttttgtgtt tatggcagta agctatgggt ggctgccttg
      721 attgatttta gcaaactgat tagggagttg cgactcatga ttagtcaatt accaaactgg
      781 aagtttttgc tggtctggag catccctttt ttatgggggg tatcccagtt aattgtggca
      841 attaaggggt agctatgtca gacaaactca taacgctggc gaagatcctc tgtgtaatcg
      901 tcggcatttc attttcacta atgctggttg ctatttttct tttcctgccc tggatgatgt
      961 tgtcttcgtc ggggatgctg gggtaacagt gactgatgac atcagcagag cgctggcttt
     1021 gctattaagt gggtggcggt tggtatcgct gtgtctccga tactgtatgg gctggcaaaa
     1081 ctggtcattg cgctgaaatc gtgaacttta tttaaacaga tgagtaatga aattgctgta
     1141 ctggtccggg caattcgctg gtggtgatat gaaccgtatt ctgtatgtgg tgattattgt
     1201 actgctggtg gcctgtggtg cgcttagtct ggggctgaat cattaccgcg ataacgccat
     1261 cacctacaaa gcgcagcgcg ataaaaaagc cagagagctg gagctagcaa acgcaaccat
     1321 tactgacatg cagcagcgcc agcgtgatgt tgctgcgctt gatgccagat actcaaggga
     1381 attagccgat gcgagagctg aaaatgaaac tctgcgcgct gatgttgccg ctggtcgtaa
     1441 gcgcctgcgg atcaacgcca cctgctccgg taccgtgcgt gaagccccca ccacctccgg
     1501 cgtggataat gcaaccgccc cccgactggc agacacagtt gaacgggatt atttcactct
     1561 cagagagcgg ttgatgctga tgcagaagca gctggaaggg gcacaggact atatccgcac
     1621 tcagtgctga actaagtttt gctgatgcgc cgtatcgtcg ccgtattccc gcataaacag
     1681 agaccgcagc caccttatct gcgtgagtgt gcgggggtaa tcaaaaacga tgcacaccgg
     1741 gttttctcat ttttcacgag atgggagcga tttcccgcga agccgcctgt ccggtgcggt
     1801 ggtggaagaa accggataaa acaacctcat tgtacaaata tcgatcaagt atggcgctgc
     1861 tgtgtgaatt ctgaaaaatc acagcggtca ttatgcgtca gtttttaaca gaggacgtca
     1921 gaaagtgaca tggcaaagct ggactggaaa aagctggagc aggcattccg acgcgaacat
     1981 gccgaaacgg gaataacatt actggactgg tgccggaaga aaaagattaa ttacaacacc
     2041 gccagaaccc gtataaaaat gggcaaaatc gatcatgaaa ttgatcataa aaccgatcat
     2101 gaaatcgatc atgacatctc agatgaagaa cccagcaatg acgcgtgttc cgacgatgaa
     2161 aaatgcgcaa aaaactctga aaaaaactgc gcaaattcgg cagaaacgaa acggattcgt
     2221 ggttcccgac ttttacctcc ttcaaacgct ttttctcagc gaaacaccca cgccgtaaga
     2281 caccgtggat atgcgaagta tcttgaggca gataacctca tggatgatgc gtccgacatg
     2341 gtgctgttcg atgaactggt gttcacccgg gcccgcgcac tttcagtaac taaggcactt
     2401 aaagggatgt tcgccgacct ggaagaggca actgacgtgg aaacccgcgt tgctctttac
     2461 gacaaaatac tcaaagctga acaggccctt gaccggaata ttgcccgtat cgagtcaatt
     2521 gaacgctcat tgctgacgct ggacgtcctg gctgagacag caccaaaact tcgtgctgac
     2581 cgggaaagaa tcaacgccgc cagagataaa ctcagagcgg aaaccgatat tctgaccagc
     2641 cagcgccggg gtgttattac gccagtcagt gacatcgtgt catcgctgca tgaaatgagt
     2701 aattcgggga gactggatga cattccggaa gaataaaacg cgatgtgatg aacctgcaga
     2761 aatgactgag accgaacaac gtctgttcat tatgacaaaa ctgagcaatc cctggtggcg
     2821 cctcaatcat ctctacaaaa tacagaacga aaaaggtgaa ctggtcacct tcagaatgcg
     2881 accggcgcaa cgtcagttgt ttcgtaacat gcataataaa aacattatcc tgaaagcgcg
     2941 ccagctggga ttttccacag ccattgatat ttatcttctc gaccaggcat tattcattcc
     3001 gcatctcaaa tgcgggatcg tcgctcagga taaacaggct gccagtgaaa ttttccgcac
     3061 aaaaattgct gtaccgtttg atcatctccc tgactggctg agagcctcat tcaccatcgt
     3121 tgaacgtcgt agcggtgcca gcggtggcta tatcctgttt ggtcacggct cgagtattca
     3181 ggtggcaacc tcatttcgct caggtacggt gcagcgcctg catatctcag agcacggcaa
     3241 aatttgcgcg aaatatccgg ctaaggcgaa agagctgcga accggtacgc ttaatgccgt
     3301 ctctgatgaa tgcattattt ttgatgaatc cacggctgaa ggcgtgggtg gtgattttta
     3361 cgagatgagt aaccgtgcac aggagagcac tgcatcaggc ttattgctga cggcacagga
     3421 ttataaattc catttttacg catggtggca ggatcctaaa tacagcgcca gagtgcctga
     3481 aagcgggctg aagctgtcac gggaaaaaat gacgtatttt tctgcggttg agaaggcaat
     3541 gaacatcacg cttaccgatg agcagaagca gtggtacatc agtaaggaaa ctgaacagcg
     3601 tgaggaaatg aagcaggagt ttccctcaac gccacaggag gcgtttctga cgtccggacg
     3661 acgtgtgttc agtgccgaaa gtacattgca ggcagaatca ttctgttcgc caccgatgat
     3721 tgtttatgac attgaacctg ttacaggagc gaagactaaa gctcagtctc tgcgtgaagg
     3781 aaataaaaac gagttgcagc ggacgctgat gaattatctg ctggtatggg aactgccgga
     3841 tccagatgaa gagtatgttt gtggggcaga tactgccgaa gggctggagc acggagaccg
     3901 ctcatcgctg gatgttgtca aacgcagtaa tggcgagcag gtggctcact ggttcgggca
     3961 tctcgatgct gaactttttg ctcatctcat ttcgcaggtc tgtcgtatgt ataacaacgc
     4021 gtttgtgggg ccggagcgta ataatcacgg acatgcagtt atcctgaaac tccgggaact
     4081 ctatccgaca cgttatatct acaacgaaca gcatcttgac caggcatatg acgacgatac
     4141 gccccgcctt ggctggctga caacccgtca gagcaaacct gttctgaccg aaggaatgaa
     4201 aacgctcctg aataatggaa tatcagggat ccgctggtca ggcacattat cggaaatgaa
     4261 cacctacgtt tatgacgcga aaggctccat gaatgcacag gaaggctgct ttgatgatca
     4321 gctcatgagc tacatgattg cccaggagat gcgcgccaga atgccggtga gggtaaaaca
     4381 gaaaacggat aaacgcagaa ccacacactg gatggcacac tgatgaaaaa tgaaattaac
     4441 accacagcga tgaaaaacga tcatggatcc acgccgcgtt tttctcagcg tcagttactg
     4501 tctctctgtt ctgatattga cagtcagcct ctctggcgtg atgccgcaaa caaggcctgt
     4561 gcgtattatg atggcgacca gctggcaccg gaagttatcc aggtactgaa agatcgcggt
     4621 cagcccatga ccatccataa cctcattgcc cccacggtag atggtgtact gggaatggag
     4681 gcaaaaacaa gaacggacct gatagtgatg tcagacgatc cgaacgatga aacagagaaa
     4741 ctggcagagg ccattaatgc ggagtttgct gatgcgtgcc gtcttggcaa catgaataag
     4801 gcccgctccg atgcctatgc ggaacaaatc aaggcggggc tcagttgggt ggaggtcagg
     4861 cgcaacagcg atccgttcgg acctgaattc agggtatcca cagtcagcag aaatgaggtt
     4921 ttctgggact ggctcagccg ggaatccgac ctgagtgact gccggtggct gatgcgtcgc
     4981 cgctggatgg ataccgatga ggcaaaagcc acgttcccgg gaatggctca ggttatcgat
     5041 tatgctattg atgactggcg tggttttgtc gataccacga ttactgaagg ccagcccagt
     5101 ccgttgatga gtgcatggga agagtatcag tcatgggatc gcagggagaa tgaatggctt
     5161 cagcgtgaac gccgtcgtgt gctgcttcag gtggtttatt accgtacatt cgagcgtctt
     5221 ccggtgattg aactcagtaa tggacgggtg gtggcttttg ataaaaataa tctgatgcag
     5281 gcggtggctg tggcatccgg gcgggttcag gtgaaagtcg ggcgggtaag tcgtattcgt
     5341 gaagcctggt ttgtgggccc acactttatt gtggatcgcc cctgtagtgc accgcagggg
     5401 atgtttccgc tggttccttt ctggggatac cgaaaggata aaaccgggga gccatacggg
     5461 ttaatttccc gcgccattcc ggcacaggat gaggtgaatt ttcgtcgtat caagctgacc
     5521 tggctgcttc aggccaaacg cgtgattatg gacgaggatg ccccccagtt gtcagacagc
     5581 gacctgatgg agcagatcga acgtccggat ggcattatta aactgaatcc ggcccgaaaa
     5641 aatcagaaaa gtgtcgcaga tgtttttcgg gttgagcagg attttcaggt tgccagccag
     5701 cagtttcagg tcatgcagga atcggaaaaa cttatccagg ataccatggg agtgtattcc
     5761 gcatttctcg ggcaggattc aggtgcgacg tcaggcgtgg ctatcagtaa cctggtggag
     5821 cagggggcca caacccttgc ggaaatcaac gataactacc agtttgcctg ccagcaggtg
     5881 ggaagactgt tgctggctta tcttctcgat gacctgaaaa aacgccgtaa tcatgcagtg
     5941 gtgattaatc gcgatgatcg ccagcgtcgc cagaccattg tcctcaatgc tgaaggtgat
     6001 aatggtgaac tgaccaatga tatttcaagg ttaaatacac atattgcgct ggcgcctgtt
     6061 cagcagacac cggcgtttaa ggcacagctt gcacagagaa tgtcagaggt tattcagggg
     6121 ctgccgcctc aggtgcaggc tgttgtgctc gacctgtggg ttaatcttct ggatgtgccg
     6181 cagaaacagg agtttgttga gcgtattcgt gctgcgctgg ggacgccaaa atcaccggat
     6241 gaaatgacgc cggaagaaca ggaagtagcg gcacaacaac aggcacttca gcaacaacag
     6301 gcagaactcc agatgcgcga gatggctggc agagtggcaa aactggaagc tgacgccgcc
     6361 agggcacatg cagctgcaca acgggataat gccagtgcac agcgggaagt cgccctgaca
     6421 caggggcagc gttatgtgga tgcgcttaac caggcacata cggcagaaat cattaccggc
     6481 gtacagaata tggaacagga gcaggacgtt cttcagcaac agatgctgta tacgttacaa
     6541 cagcggatga atgaaatgtc gctctgaaaa ctctggcttc aactgaaccc cgtcatcgta
     6601 cggggttttt tgtttccgga ggtaagcgtt ccgggagcgg tgcgcttatt cgcgggggca
     6661 gcgataagcc ttatttactc aaccattcgg atctgtccga taaacagacc atgcggagtt
     6721 atttatggat tttgaattta cgggtgaaga aaccccggaa caactggaaa aaatgctgga
     6781 aggacttggg gatgtggata ttgacagtca cgcacaggac gtcgtgacgg aagataccac
     6841 ggaaaaacat gcggatgagg aagcacagac tcagacgggc gataacaatg tggcaccgac
     6901 gccggatgcc agtgtggagc agacgcagga cgtgaaggag ccggaagcga agggggtgct
     6961 cacccgcgac ggtaaacacg tcattcccta tgaagtcctt gaggctgaac gttccggtaa
     7021 gcaacgggcc gaacaggaag ccgcacttct tcgtgggcag atagctgaag aaaaacgcag
     7081 ggtggaacta ctgacgtctc agatccacca ggccggtatg aagcccacac cgttaccgga
     7141 aaacgaaaaa atttctgatg agcagattgc ccgtatcagg gagatgtatc cggaaattgg
     7201 tgacgcggtg gcttcgctca tccgtaaaaa taactatctc cagtcccgtg ttcagcaatc
     7261 aacacagcag gcagaaggta atggtggtga ggatttatca ccggttcttg atgcgatgaa
     7321 tgccgtgccg gtgctgaaaa cgtggcagga gtccgatccg gatcgcttct cggttgctgt
     7381 atccatcgac gggaagctcc agaatgaccc cgcatggaaa gacaaaacgc tcactgaacg
     7441 tttcgctgaa gtggcccgtc gtacgcaggt tgctttcggt gaagtcagtg agtcgtctgc
     7501 tgacaaccag gcagacaaaa cggatatccg gaaaacggcg gaagagaaag tgaagaccgc
     7561 tgaacaggag caggcagtac ctgcttcccc gtcagattta ggcaccacgg cctccgtcgg
     7621 aatcggtgat aattttgaac ggttacttgg tgcttctcat tcagaggctg aggcgattat
     7681 gcgtggtatg acgaatgctg aaatagacgc acttctggag aagctcgggt aacttactga
     7741 aggagtactg aagtaatgac gactgtaaca tcagcccagg cgaataagct gtatcaggtg
     7801 gcgcttttta ctgctgccaa ccgcaaccgc tcgatggtta atatccttac tgaacagcag
     7861 gaagcgccaa aagcggtttc gccggacaag aaaagcacga agcagaccag cgcaggtgcg
     7921 ccggttgtcc gtatcacaga ccttaacaaa caggccggtg atgaagtgac cttcagcatc
     7981 atgcacaaac tctcaaaacg tccgacgatg ggagatgagc gtgttgaagg tcgtggtgag
     8041 gatctcagcc atgctgactt ctccctgaaa atcaatcagg gacgtcacct ggtggatgca
     8101 ggcggacgta tgagtcagca gcgcacgaag tttaacctgg catcctctgc cagaatgctt
     8161 ctggggacgt actttaatga cctgcaggac cagtgtgcga tagtgcatct tgctggagct
     8221 cgtggtgatt ttgttgctga cgacactatt ctgccgacag cggagcaccc tgaattcaaa
     8281 aaaatcatga tcaacgatgt actgcctccg acacatgacc gtcacttttt tggcggtgat
     8341 gcgacaagct ttgagcagat tgaagcggca gatatttttt ctattggcct ggtggacaat
     8401 ctctccctgt tcattgacga aatggcgcat ccgttacagc cggttcgtct gtccggtgat
     8461 gaacttcacg gagaagatcc atattacgtc ctgtacgtca cgccgcgtca gtggaatgac
     8521 tggtacacct cgacgtccgg taaggactgg aaccagatga tggttcgggc cgtgaaccgt
     8581 gcaaaaggtt ttaatcatcc gctgttcaaa ggtgaatgtg cgatgtggcg caatatcttg
     8641 gttcgtaagt atgcgggtat gccgatccgt ttctatcagg ggtcaaaggt tctggtatca
     8701 gagaataacc tgacggcaac cacgaaagag gtcgctgctg caaccaatat tgaccgcgcc
     8761 atgttactgg gggctcaggc gctggcaaat gcttacggtc agaaggcggg cggtcacttc
     8821 aacatggttg agaagaaaac ggatatggat aaccgtactg agatagcaat cagctggatc
     8881 aacggtctga aaaaaatccg tttccccgag aagagcggca agatgcagga tcacggcgtg
     8941 attgccgttg atacagcagt gaagctctga ttttttcctt tccccatgcc gggttttcgc
     9001 ccggcttttt caggagtcat taattatggc aaagactatc cttgccccgt cactgagtga
     9061 acgggtctat acgggtacgc acggtaatga gtcggtggca gaaggcgtat ttacggtgaa
     9121 tgctgcggaa gcggacagtg ttattcatct tctctcactg ccagtgggca tccgtatcaa
     9181 ctcactccag ctggtttcaa cgggtggtct gggtactgca accgtcagca ttaagtccgg
     9241 tgagcatgct ctcatcgata acagcgaagc tgtttctgca aaatttgcca gatatgtgcc
     9301 agtggagccg tacaccacac agcgtgacgg ggagctggtt actgtcacca ttaagactgc
     9361 cgctgcaacc ggcaccctga atgttctgct gcgttatacc gtggtgggat actgattaaa
     9421 accttccggc ccgcgtcatg cgggcttttt atccggggaa ttatatgagt gagaaaattg
     9481 ccgttgtcta tatcggccca aaacccgtga aaaaggacac cattaccgga agccgcacat
     9541 tgttcccacg tcttgagccg gtgcatgttg acagtgcgat ggcctggcaa ctgctggggt
     9601 ttccggatgt ctgggttcgt catgaagagc ttgatgatgt tctgaaaaag caacaacaga
     9661 atgagcagtt gcggcaggca cagcaggcgc aggaaagagt gcttgctgcg cgggcagaag
     9721 cggagaacag ttttgttgtt tctgttaacg ggcaggaggt ggatttaagt aagctcacct
     9781 cagcacggct ggcgacgctg tgtgaggcag aagagctgga tattcacaaa gatccgaaag
     9841 aaacggctga ggcattccgt atccgggtgc gtgaggcatt tcgccgtcgt gttgcggaga
     9901 ctgaacagca tggcggaact gagtgatttt ttaccgtatg tccgtcgtca gatcagcggt
     9961 ccactgaaca ttatgatgac ggatgctctg tcaatggctg ccgtggcatt cagccgtcag
    10021 tcgctggtgt gccgtcggga ggttactgtt gtaccagtag caggaaaaga aatcgtgctt
    10081 ccgtatgaca aagatgatga ggagtgcgtt catatcatcc gtatctctga cgataatcat
    10141 gagctttttg tcggtcggga tgtggatatc agctccggac gctccctgcg atttgcctgt
    10201 tctcccggtg aggtgagcgt gctttatgcc gtcgctccga aagccggacg cagccagata
    10261 ccggatgaac tcctcacatg gcctgaagaa gtggctgcgg gggcacttga gcggttgttc
    10321 atgcagactg gtgtttcatg gtcagatccg tcacgcgcac agtatttttc tgtgcagttt
    10381 tctgagggga tccgtcgggc atatcgtcat acactggcga caagcccgta ctcttcatac
    10441 cgcaaccctg tacgcaggca gaggttttac tgatgacgac gattacagaa atcatcggac
    10501 gtgtgaatac acaactggtt gacccgatga tggtgcgctg gcctctggct gagctgtgtg
    10561 attattacaa tgatgctgtg cgggcggtga ttctggcaag gccggatgca ggcgcaagcc
    10621 tggaaacgct cagttgtgtc cctggcgcac gccagacttt acctgatggc gcaatacagc
    10681 ttcttgacgt gatatgcctc agcgatggca gtgcgattaa accagtatcc cgggaggtgc
    10741 ttgatgcaca gtatcccgac tggcacatgc tgaggggaaa accggaatgc tttatcagca
    10801 acgacctgtc cccgcgcgta ttctggctgt ttcctgtccc tgacaaagag ataagtattg
    10861 atgcagtggt aagccggata ccggaggcag tgtatgttct gacgcaggac gatgatacgc
    10921 cagttccact ggaagaggct tatgttaacc cactggtgga ctggatgttg tttcgtgctt
    10981 tcagtaagga tgctgccggt ggtgcagaat cggggctggc tgcgcagcat tatcagagtt
    11041 ttgttgagca acttgggatc aaacaggggg cagacagtgc attgtctgcc cgtaaaaaag
    11101 tgtttaacgg aggtggagtg tgagtgttgt tgtttcgggg acgctgaaat ctcctgatgg
    11161 tgaggcgata tcaggagcaa atattaccct gacggcgctg acagtttcac cggatgcgct
    11221 cagcggcacc agtgcgtcgg cagtgacccg tgaaggtgga tattacggaa tgacgatgga
    11281 tccgggggag tatgcggttt cggtgacggt gaaagggaag actgctgtct acggacgtgt
    11341 gcgtattgag gggaccgaaa gtacggtgac gctcaatatg ctgttacgcc gcagtcttgt
    11401 tgaggtgagc atacccggag agctgctgac agatttccgg cagatacaga ataatgtggc
    11461 tgatgacctt gctactattc gtcgcctgaa tgaagacacg gcgacaaaaa acactcaggc
    11521 cacacagtca aaagaaagtg cagcagccag tgcgaagagt gcatctgaca gtgcaaagac
    11581 ggcaaccagc agggcggctg aagccggaca aaaagcgact gatgccactg aggccgcgac
    11641 ccgtgcaatc acagcagcgg ggaatgcaga ggaaagctcg acccgtgccg gagagtctga
    11701 aaaagccgcc ggagctgatg cagaaaaagc cagacagcat gctgaaaagg ccaggctggc
    11761 gcaggagagc gccggagaga tccttaagcg ggcagaggct gccactgtca gtgctgaaga
    11821 ggccagacgt atggctgaga atgcacgggg gccccggggg cctcagggag aaactggtcc
    11881 gaagggggat gtcggtccta aaggcgaaac aggtccagtg ggccctcaag ggcccgcagg
    11941 gccgaaaggt gagcgtggtg acgttggtgc tcagggggct gtagggcccg ctggtccgcg
    12001 tggtgagaag ggcgaacagg ggaagcgagg accgcaggga ataccaggcc tgaag
//
LOCUS       DAZGXZ010000144        11934 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000144 DAZGXZ010000000
VERSION     DAZGXZ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11934
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.144"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..353
                     /locus_tag="ACHKJF_004314"
     CDS             <1..353
                     /locus_tag="ACHKJF_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0951567.1"
                     /translation="NNCPTMQLMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNE
                     LFHYPIKKAYTPYNIHTYKTEEVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPI
                     DLLDRSFSSSSWPF"
     gene            677..2065
                     /gene="narU"
                     /locus_tag="ACHKJF_004315"
     CDS             677..2065
                     /gene="narU"
                     /locus_tag="ACHKJF_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP0951568.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAILGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2147..5887
                     /gene="narZ"
                     /locus_tag="ACHKJF_004316"
     CDS             2147..5887
                     /gene="narZ"
                     /locus_tag="ACHKJF_004316"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP0951569.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSSSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5884..7428
                     /gene="narH"
                     /locus_tag="ACHKJF_004317"
     CDS             5884..7428
                     /gene="narH"
                     /locus_tag="ACHKJF_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0951570.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVMEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7428..8123
                     /gene="narW"
                     /locus_tag="ACHKJF_004318"
     CDS             7428..8123
                     /gene="narW"
                     /locus_tag="ACHKJF_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0951571.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8120..8800
                     /gene="narI"
                     /locus_tag="ACHKJF_004319"
     CDS             8120..8800
                     /gene="narI"
                     /locus_tag="ACHKJF_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0951572.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLVQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8879..9772
                     /gene="yddE"
                     /locus_tag="ACHKJF_004320"
     CDS             8879..9772
                     /gene="yddE"
                     /locus_tag="ACHKJF_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP0951573.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARKLGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9868..10713)
                     /gene="nhoA"
                     /locus_tag="ACHKJF_004321"
     CDS             complement(9868..10713)
                     /gene="nhoA"
                     /locus_tag="ACHKJF_004321"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP0951574.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10886..11455
                     /gene="yddH"
                     /locus_tag="ACHKJF_004322"
     CDS             10886..11455
                     /gene="yddH"
                     /locus_tag="ACHKJF_004322"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP0951575.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11459..11686)
                     /gene="pptA"
                     /locus_tag="ACHKJF_004323"
     CDS             complement(11459..11686)
                     /gene="pptA"
                     /locus_tag="ACHKJF_004323"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP0951576.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11785..>11934)
                     /locus_tag="ACHKJF_004324"
     CDS             complement(11785..>11934)
                     /locus_tag="ACHKJF_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0951577.1"
                     /translation="AELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLA
                     GRRLS"
BASE COUNT         2852 a         3088 c         3104 g         2890 t
ORIGIN      
        1 agaacaattg ccctactatg cagctcatgt tatttaattt gttttcccca gcacttaagc
       61 ttaatactgg cctggcaatt ctttcgcctg gtgcatttga agttcactct gacggaatag
      121 atgtggataa cgaattgttt cactatccta ttaaaaaagc atatacccca tataatatac
      181 acacttacaa gacagaggaa gttgtaaacc agaggaatat aaaagttaaa aacatgacct
      241 tagatgaaat aaacaatact tactgtaata acgattatta caatcaggca ataagagagg
      301 aaccgataga ccttctggac agatcgtttt cctccagttc atggcctttt tagtgaataa
      361 atcatcacta attctataag tgtctggtct tgttgataat atagttaata tgtaaccctt
      421 tagattcacg atctgaaggg ttgaaacatg acatacgttc aaagattaac ccccgtttga
      481 aggttagctt gttagttaca tttagtaaca catatctggc tacttaatcc ccattctgcc
      541 cccgcggtta cgctttctta tctattcttt caatcagcac ttcacccaat tcatccttaa
      601 ttctaataac tggctattgc gccttacctc ctgttttgtc ttttttcatt accaatgtgt
      661 gcatgtgagg aacaatatgg cactgcaaaa tgagaaaaat agtcgttatc ttttacgcga
      721 ctggaaacca gaaaatccgg ccttctggga aaataaagga aaacatattg ctcgaagaaa
      781 cctctggata tcagtcagtt gtctacttct tgccttctgt gtctggatgc tatttagcgc
      841 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
      901 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      961 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
     1021 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
     1081 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
     1141 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
     1201 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgccattct
     1261 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
     1321 gatttgggta ccgctactgg cgattgccac gatcgccgca tggtcaggga tgaatgatat
     1381 cgccagttca cgcgcctcaa ttgccgacca gctccctgtc ttacaacgcc tgcatctctg
     1441 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
     1501 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
     1561 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata agttcggcgg
     1621 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
     1681 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
     1741 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
     1801 ccagataacc atttatcggg taaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1861 agccgtcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1921 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1981 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     2041 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     2101 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     2161 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     2221 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     2281 cgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     2341 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacct
     2401 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctttacag
     2461 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgcgaagc
     2521 cctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     2581 gtgcctgagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     2641 actaaaccag ctgattgccg ccgctaacgt ctggaccatc aaaacctacg gcccggatcg
     2701 cgttgccggt ttctcgccga tcccggcgat gtcgatggtt tcttacgccg ccggaacgcg
     2761 ttatctgtcg ctgcttggcg gcacctgttt aagtttctac gactggtatt gcgacctacc
     2821 gcccgcctcg ccgatgacct ggggcgagca aaccgacgta ccggaatctg ccgactggta
     2881 taactccagc tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2941 ccacttcttt accgaagtac gctacaaagg cactaaaacc atcgccatta cccctgacta
     3001 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     3061 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     3121 ctactttatc aactactgcc gccgctacag cgacatgccg atgctggtaa tgctggagcc
     3181 tcgcgacgat ggtagctacg ttcccgggcg catgatccgc gcatctgacc tggtggatgg
     3241 actgggcgaa agcaacaatc cgcagtggaa aaccgtagca gttaataccg caggtgaatt
     3301 ggtagtgccg aacggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     3361 atccattgcc gccggtacgg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     3421 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt
     3481 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     3541 caacacctgt ccggtggtca gcgtttatga tttggtactg gcgaattacg gcctcgatcg
     3601 cgggctggaa gatgaaaaca gtgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     3661 ctggggtgag caaattaccg gcgtgccgcg ccagtatatt gaaaccatcg cccgtgaatt
     3721 tgccgatacc gcccataaaa cgcatgggcg ctcgatgatt atcctcggcg caggtgttaa
     3781 ccactggtat cacatggaca tgaactaccg cgggatgatc aatatgctga tcttctgcgg
     3841 ttgtgtcggg caaagcggtg gcggctgggc acactatgtc ggccaggaaa aactgcgccc
     3901 acaaaccggc tggttgccac tggcctttgc gctcgactgg aaccgaccac cgcgccaaat
     3961 gaacagcacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     4021 tcaggagtta ctttcaccgc tcgccgatgc cagtaagtac agcggtcatc tgattgattt
     4081 caacgttcgc gccgaacgta tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     4141 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     4201 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagca gcagcaatca
     4261 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     4321 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     4381 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcagcgattg aaggcaagct
     4441 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     4501 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     4561 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     4621 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     4681 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     4741 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     4801 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4861 gatggacaaa cttggcaatg gcggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4921 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4981 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     5041 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     5101 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     5161 aattatctcc agccccacct ggtccggtct ggaaagcgat cacgtatcct ataacgcggg
     5221 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     5281 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     5341 cgacactcgt agcgtcagtg agatgcgcca gataccgcca aacggcttcc cggaaaaagc
     5401 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     5461 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa cagatgcccg
     5521 agaactgacc attgtcgata acgactgggt ggaagtattc aacgccaacg gcgcgctgac
     5581 tgcccgcgcg gtggtcagcc aacgtgtacc gccgggcatg accatgatgt atcacgccca
     5641 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     5701 ctcggtcacc cgcgtttgcc cgaaaccaac gcatatgatt ggcggttacg cgcagctggc
     5761 ctggggcttt aactactacg gcaccgtcgg atcgaaccgc gatgagttca tcatgatccg
     5821 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5881 aaaatgaaaa tccgttcaca agtcggcatg gtgcttaacc tcgacaaatg tatcggctgc
     5941 catacctgtt cggtgacctg taaaaacgtc tggaccgggc gcgaaggcat ggagtacgca
     6001 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     6061 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatccgtcc gcgtctgggc
     6121 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     6181 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     6241 atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa agtgatctgg
     6301 ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     6361 ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat gatgtacctg
     6421 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     6481 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     6541 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     6601 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     6661 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatc
     6721 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     6781 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6841 gtgatggaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6901 ctaccgctgc accctgaata tcgcaccctg ccgatggtct ggtacgttcc tccgctgtcg
     6961 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     7021 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     7081 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     7141 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     7201 gtcgaagaga tgtatcgtta cctcgccatt gccaactatg aagatcgttt tgtcatcccg
     7261 acgagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     7321 tttggcgacg gttgtcacgg ctcggacagc aaattcaacc tgttcaacag tagccgtatc
     7381 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     7441 aagtgatcgg cctgttgatg gagtatccgg acgagctgtt gtgggaatgc aaggaggacg
     7501 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     7561 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     7621 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     7681 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     7741 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     7801 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7861 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7921 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7981 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     8041 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcaa tatgtcgaca
     8101 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     8161 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acggccagta
     8221 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     8281 tctgttccat atcggcattt tggggatttt cttcgggcac ctgttcggca tgttaacgcc
     8341 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     8401 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcaggct
     8461 gacaaatcag cgcgtgcgtg ccacttccac tacaccggat atcatcatca tgagcatttt
     8521 gctggtccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     8581 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     8641 gtcagagatg cttaacggtg tagcgttcgt tttccgcctg catctggtgt tgggaatgac
     8701 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     8761 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     8821 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8881 gaaaccgcag gtttaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8941 cggtgttgtt ttccccgccg ataatcttag cgaagcgcaa atgcagctta tcgcccgcaa
     9001 attaggtcat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     9061 ctactttacg ccaacagttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     9121 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacggtc tggcaaacat cgctggcagg
     9181 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     9241 tacgccgggc tttgaaccgc cgctggaggg tgaaacacgt gaggcgatta tcaacgcgct
     9301 tcatctcact gaggacgata ttctgccagg cttgccaatt caggtggcaa caacaggaca
     9361 ctcaaaagtg atgatcccgt tgaaaccaga agtggatatc gacgcccttt cgcctgatct
     9421 tgctgcgctg agcgctatca gcaaacagat tggctgcaat ggtttcttcc cgttccagat
     9481 ccgcccaggc aaaaacgaaa ccgatggtcg catgttctcg cctgcgattg gtattgtgga
     9541 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     9601 attgccccac gatggcaagg tgctgcgtgt taaaggccat caggggcgcg cattggggcg
     9661 tgacggcgtg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     9721 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     9781 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9841 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9901 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9961 accaaactgt tcctgcatca cggcatataa cgacaccaca tccggtagat ttcgctgctc
    10021 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagcgtta gcttgccacc
    10081 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
    10141 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat caaaacggta
    10201 catcgactgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
    10261 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
    10321 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcctctt ccagttccac
    10381 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
    10441 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
    10501 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
    10561 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
    10621 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
    10681 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
    10741 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
    10801 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10861 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10921 cagccgtcta ttaaatcatg gcccaactgt catgatcacc agtttcgatg agcaatccca
    10981 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac cgccacgcgt
    11041 ggcgattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    11101 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcagtgg gaagcgtgtc
    11161 gggtcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    11221 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    11281 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    11341 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    11401 tcatttaggt gctggcacgt ttgttaccag cggcaagcga gttacggcgg gttaagcatt
    11461 acatgctgta accgggtttc tttatcaaag cctccatttg gggcgcgatt tcggcatccc
    11521 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    11581 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    11641 gttcgtccag ttcacgcgga aaacatttaa tgtcgatgtg cggcatgatt gtgctccttt
    11701 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtcg tgtgtccggc aatgcgtttt
    11761 tacgacaata atgccagggc aagattacga aagccttctc cccgcaagga ctgacgccag
    11821 gtagtttctg tccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
    11881 atcattcgtc aaaatattaa tagcaatgtg ccgtatccct gaaaataatt ctgc
//
LOCUS       DAZGXZ010000145        11915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000145 DAZGXZ010000000
VERSION     DAZGXZ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.145"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..791
                     /gene="ydeO"
                     /locus_tag="ACHKJF_004325"
     CDS             30..791
                     /gene="ydeO"
                     /locus_tag="ACHKJF_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP0951578.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMNALAI"
     gene            1195..2877
                     /gene="ydeN"
                     /locus_tag="ACHKJF_004326"
     CDS             1195..2877
                     /gene="ydeN"
                     /locus_tag="ACHKJF_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0951579.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2929..4086
                     /gene="ydeM"
                     /locus_tag="ACHKJF_004327"
     CDS             2929..4086
                     /gene="ydeM"
                     /locus_tag="ACHKJF_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0951580.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKNLFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4052..4174)
                     /locus_tag="ACHKJF_004328"
     CDS             complement(4052..4174)
                     /locus_tag="ACHKJF_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951581.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKGRVINYSPFVLQHP"
     gene            4377..6062
                     /gene="yddA"
                     /locus_tag="ACHKJF_004329"
     CDS             4377..6062
                     /gene="yddA"
                     /locus_tag="ACHKJF_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP0951582.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            6100..8472
                     /gene="yddB"
                     /locus_tag="ACHKJF_004330"
     CDS             6100..8472
                     /gene="yddB"
                     /locus_tag="ACHKJF_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP0951583.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKMHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8529..11312
                     /gene="pqqL"
                     /locus_tag="ACHKJF_004331"
     CDS             8529..11312
                     /gene="pqqL"
                     /locus_tag="ACHKJF_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0951584.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            11674..>11915
                     /locus_tag="ACHKJF_004332"
     CDS             11674..>11915
                     /locus_tag="ACHKJF_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0951585.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         3535 a         2571 c         2547 g         3262 t
ORIGIN      
        1 taaaaacttt attgttttaa ggagataaaa tgtcgctcgt ttgttctgtc atatttattc
       61 atcatgcttt caacgctaac attttagata aagattacgc cttctctgac ggcgagatcc
      121 tgatggtaga taacgctgtt cgtacacatt ttgaacctta tgagcggcat tttaaagaga
      181 tcggatttac tgaaaatacc attaaaaaat atctacaatg cactaacatc cagacagtga
      241 cgatgcctgt tcctgcgaag tttttacgtg cttcaaatgt accgactgga ttgcttaatg
      301 aaatgattgc ttatctcaac tcggaagaac gcaatcatca taatttttca gaacttttgc
      361 ttttttcttg cctgtctatt tttgccgcat gcaaaggttt cattacacta ttaactaacg
      421 gtgtgctatc cgtttccggg aaagtgagaa atattgtcaa catgaagctg gcgcacccat
      481 ggaagctgaa agatatttgt gactgcctgt acatcagtga aagcctgttg aagaaaaaac
      541 taaagcaaga gcaaacgaca ttctcacaga ttctattaga tgccagaatg cagcacgcaa
      601 aaaatttgat acgcgtagaa ggttcagtca ataaaattgc cgaacaatgt ggttatgcca
      661 gtacatctta ttttatttat gcgttccgca aacatttcgg caacagtccg aagagagttt
      721 ctaaggagta ccgttgtcaa agtcacatgg gtatgaatac gggcaacacg atgaatgctt
      781 tagctatttg attatttgct aacgagtagt caaccacgca ccctacgtaa aaattaaaaa
      841 tggggcagtc attccctgcc ccgcgttgtt tttaggtgat atatttattg aaataaataa
      901 gtgacattca tcacatattt atgcatttgc ataacctgtt gcatgattat ttatgatctc
      961 aattcggcat tttgtcagta aaatgcaata atttattaaa tatcaataga ttagttgttt
     1021 atcggcgaga aattacttag tagaacagaa agtaatgtca acgctttatg gactgttatt
     1081 ttcctttttt tagctaaatc tgctatctct ttatgtgact aacttcactt acatccactt
     1141 atttctcttc gtaaaattac tttggaatta agtacaataa gaagaggaac atttatgaag
     1201 tctgcattaa agaaaagtgt cgtaagtacc tcgatatctt tgatactggc atctggtatg
     1261 gctgcatttg ctgctcatgc ggcagatgat gtaaagctga aagcaaccaa aacaaacgtt
     1321 gctttctcag actttacgcc gactgaatac agtaccaaag gaaagccaaa tattatcgta
     1381 ctgaccatgg atgatcttgg ttatggacaa cttccttttg ataagggatc ttttgaccca
     1441 aaaacaatgg aaaatcgtga agttgtcgat acctacaaaa tagggataga taaagccatt
     1501 gaagctgcgc aaaaatcaac gccgacgctc ctttcattaa tggatgaagg cgtacgtttt
     1561 actaacggct atgtggcgca cggtgtttcc ggcccctccc gcgccgcaat aatgaccggt
     1621 cgagcgcccg cccgctttgg tgtctattcc aataccgatg ctcaggatgg tattccgcta
     1681 acagaaactt tcttgcctga attattccag aatcatggtt attacactgc agcagtaggt
     1741 aaatggcact tgtcaaaaat cagtaatgtg ccggtaccgg aagataaaca aacgcgtgac
     1801 tatcatgaca acttcaccac attttctgcg gaagaatggc aacctcaaaa ccgtggcttt
     1861 gattacttta tgggattcca cgctgcagga acggcatatt acaactcccc ttcactgttc
     1921 aaaaatcgtg aacgtgtccc cgcaaaaggt tatatcagcg atcagttaac cgatgaggca
     1981 attggcgttg ttgatcgtgc caaaacactt gaccagccat ttatgcttta cctggcttat
     2041 aatgctccgc acctgccaaa tgataatcct gcaccggatc aatatcagaa gcaatttaat
     2101 accggtagtc aaacagcaga taactactac gcttccgttt attctgttga tcagggtgta
     2161 aaacgcattc tcgaacaact gaagaaaaac ggacagtatg acaatacaat tattctcttt
     2221 acctccgata atggtgcggt tatcgatggt cctctgccgc tgaacggggc gcaaaaaggc
     2281 tataagagtc agacctatcc tggcggtact cacaccccaa tgtttatgtg gtggaaagga
     2341 aaacttcaac ccggtaatta tgacaagctg atttccgcaa tggatttcta cccgacagct
     2401 cttgatgcag ccgatatcag cattccaaaa gaccttaagc tggatggcgt ttccttgctg
     2461 ccctggttgc aagataagaa acaaggcgag ccacataaaa atctgacctg gataacctct
     2521 tattctcact ggtttgacga ggaaaatatt ccattctggg ataattacca caaatttgtt
     2581 cgccatcagt cagacgatta cccgcataac cccaacactg aggacttaag ccaattctct
     2641 tatacggtga gaaataacga ttattcgctt gtctatacag tagaaaacaa tcagttaggt
     2701 ctctacaaac tgacggatct acagcaaaaa gataaccttg ccgccgccaa tccgcaggtc
     2761 gttaaagaga tgcaaggcgt ggtaagagag tttatcgaca gcagccagcc accgcttagc
     2821 gaggtaaatc aggagaagtt taacaatatc aagaaagcac taagcgaagc gaaataacta
     2881 aaccttcatg cggcggaatt ttccgccgcc ttattgagcg agatagcgat gcacgttaca
     2941 gccaagccct ccagttttca atgtaatctc aaatgtgatt actgttttta ccttgaaaaa
     3001 gagtcgcagt ttactcatga aaaatggatg gatgacagta ctctgaaaga gttcatcaaa
     3061 caatatatcg cagcgtctgg caatcaggtc tattttacct ggcaaggcgg tgaacccact
     3121 ctggctggcc tggatttttt ccgtaaagtt attcactatc aacaacgcta tgcaggccaa
     3181 aaacgtattt ttaatgcatt acaaacgaat ggcattttat tgaataatga atggtgtgct
     3241 tttctcaaag aacatgagtt tctggttggt atctcgatcg atggccccca ggagctacat
     3301 gaccgttaca gacgcagtaa ttcaggtaac ggtacttttg caaaagtgat agcagccatc
     3361 gagcgtctga aatcatatca agttgagttt aatacgttaa ccgtcattaa taacgttaat
     3421 gtccattacc ctcttgaggt ttatcatttt ttaaaatcta tcggcagtaa acatatgcaa
     3481 tttatcgaat tgctcgaaac cgggacgccg aatattgatt tcagtggtca tagtgagaac
     3541 acattccgta tcattgattt ttctgtgcct cccacggctt atggcaagtt tatgtcaacc
     3601 atttttatgc aatgggttaa aaacgatgtg ggtgaaattt tcattcgtca gtttgaaagc
     3661 tttgtcagcc gttttttggg gaatgggcat accagttgta ttttccagga gtcctgcaag
     3721 gataatctgg ttgttgaaag taatggagac atttacgaat gcgaccattt tgtctatcca
     3781 cagtacaaaa ttggaaacat taataaatct gaactcaaaa cgatgaacag tgtacaactg
     3841 acagcgcaaa aaaaacggat ttcagcgaaa tgtcagcaat gtgcatataa acctatctgc
     3901 aatggcggtt gtcctaagca tcgtattact aaagtaaaca atgagactgt ttcttatttt
     3961 tgcgaaggtt ataaaaacct tttttcaacc atggtacctt atatgaacgc catggtggag
     4021 ttagctaaga acagagtacc gctttatcac attatggatg ttgcaaaaca aatggagaat
     4081 aattaataac acgccctttt agctccattc ttagcctcct tcttatgacc ttaacaaggc
     4141 acccgacata aattcctgtc gggtgtttcg tcatcatcat cttcccttgc gaaaatcctg
     4201 gcttacgtgg tatgcgtcag ggcattagca atacccttga ttatcaatag cataaccata
     4261 actaatgaaa atgaaatatt tctttatatt tcatattgtt aagtgatact taaataaatt
     4321 tcatcagtaa atcattgcca tagataatca ttggatggca caatcaattt tcgctaatga
     4381 taaccattcc cattacgttt tgtatgttga tagcaaagta tctctgtctg ctgaagccct
     4441 tttggctgcg taagaataat aaaacttcag ttttgttaat cattattatc ctcgccatga
     4501 tccttggcgt ggttaaaatt caggtctggt taaacgactg gaataatgat tttttcaatg
     4561 cgctaagcca gaaagaaacc gataagcttt ggcagcttgt cctttggttc cctgccctgc
     4621 tggggatctt tgtattaata tccgtaaaca aaacatggtt aattaaacta ctgactattc
     4681 gctggcgcga atggttaaca gattactacc ttaatcgctg gtttgcagat aaaaactact
     4741 acttcacgca gatctacggc gagcataaaa atacagataa ccctgaccaa cgtatcgctg
     4801 aagacattct cttactgatc agcaaaacgc tcagcctttc atttggcttc atccagtcac
     4861 ttagcatgct gatcaccttt accgttattc tttggcaaag cgcgggtacg ctctcattca
     4921 ctgttggtgg aacagaatgg aatatccagg ggtatatggt ctataccgtt gtgctcattg
     4981 tgatcggtgg aactttattt acccataagg ttggtaagcg tattcgtccg cttaatgtgg
     5041 aaaaacaacg tagcgaagcg acatttcgaa ctaatcttgt gcagcataat aagcaggcag
     5101 aactgattgc gctaagcaat gctgagtctc tacaacgcca ggagttgagc gacaattttc
     5161 ataccattaa agagaactgg catcgtttaa tgaatcgtca gcggtggctt gattactggc
     5221 aaaatattta ttcgcgctcg cttagcgttc ttccctactt tctgttattg ccgcagttta
     5281 ttagcgggca gatcaatctg ggcggactga tgaaatcgcg ccaggcattt atgctagtat
     5341 cgaacaattt aagctggttt atttataaat atgatgaact tgctgaactg gctgcggtta
     5401 tcgatcgctt gtatgagttc catcaactca ctgaacagcg ccctacgaat aagcctaaaa
     5461 attgccaaca tgcggtacaa gtggctaatg cgagtattcg tacgcctgat aataagatca
     5521 tattagagaa cttgaacttt catgtttcgc caggcaaatg gctattactg aaaggctact
     5581 ctggtgcggg aaaaaccaca ctgcttaaaa cattatccca ctgctggccg tggtttaaag
     5641 gtgatatttt ttctcctgct gacagttggt atgtgtcaca aacaccgtta atcaaaaccg
     5701 gcttactgaa agagattatt tgtaaagcac ttcccctgcc cgtagacgat aaatcgttga
     5761 gcgaagtact gcatcaggtt ggtctgggga aattggctgc gcgtattcat gaccacgatc
     5821 gctggggaga tattctttcc agcggcgaaa aacaacgtat cgccctggca cgattaattt
     5881 tacgacgtcc gaaatggata tttcttgacg aaactacctc tcatcttgag gaacaagagg
     5941 ctatacgctt actgcgttta gtgcgtgaaa aactacccac aagcggcgtc attatggtta
     6001 cacatcaacc cggtgtctgg aacctggctg atgatatttg tgacattagc acggttttat
     6061 aacaacaata aaagccctgc tgtacggaga tataaataaa tgaagcgagt tcttattcct
     6121 ggcgtcattt tatgtggcgc tgatgtggcg caggccgtcg atgacaaaaa catgtacatg
     6181 catttttttg aagagatgac ggtctatgct cctgtccctg tacccgtaaa cggcaacacg
     6241 cattatacca gtgaaagcat cgagcgttta ccgaccggga atggcaatat cagcgatctg
     6301 ctgagaacca accctgcggt acgcatggat tcaacgcaaa gtacctcgtt gaaccaggga
     6361 gatattcgcc ctgagaaaat ctctattcac ggtgcgtcgc cctaccagaa tgcctatttg
     6421 attgacggta ttagtgcaac taataacctg aacccagcga atgagtccga tgccagtagt
     6481 gcaaccaata ttagcgggat gtcacagggg tattatcttg atgtcagctt actggacaat
     6541 gtgacgcttt atgacagttt tgtgccggtt gaatttggtc gcttcaatgg cggggtaatt
     6601 gatgcaaaga tcaaacgctt caacgctgat gatagcaagg tgaaattggg ttatcgcact
     6661 acgcgttcgg actggttaac atcgcatatc gatgagaata acaagagcgc atttaatcaa
     6721 ggctcttcag gaagtactta ttactcccca gattttaaaa agaactttta taccttgtcg
     6781 tttaatcagg aactcgctga taacttcggc gttaccgccg gtttatcgcg ccgccagtct
     6841 gatatcaccc gcgcggatta tgtttcgaat gacggcattg tcgccggtcg ggcacagtat
     6901 aaaaacgtta tcgatactgc attgagcaaa tttacctggt ttgccagcga ccgctttacc
     6961 cacgatttaa ccttaaaata taccggctcc agtcgtgatt ataataccag caccttcccg
     7021 cagtctgatc gcgaaatggg taataaatcc tacggtctgg catgggatat ggatacgcag
     7081 ctcgcatggg ccaaactgcg taccaccgtt ggttgggatc atattagtga ttatacccgt
     7141 cacgatcatg acatctggta caccgaactt tcatgtacat atggtgatat tacagggcgt
     7201 tgcacccgtg gcggattagg acacatttcc caggctgtag ataattacac cttcaaaaca
     7261 cgcctggact ggcaaaaatt cgccgtgggt aatgtttcgc atcaacccta cttcggcgcg
     7321 gaatacatct attccgatgc gtggactgaa cgccataacc agtctgaatc ctatgtgatt
     7381 aatgctgccg gaaagaaaac taaccatacc atttaccata aaggtaaagg cagcctggga
     7441 attgacaact acacgctgta tatggcggat cgcattagct ggcgtaatgt gtcattaatg
     7501 cccggtgtgc gttatgacta tgacaactat ctgtcaaacc acaatatctc cccgcgcttt
     7561 atgacggaat gggatatttt tgctgatcaa acctcaatga ttacagcagg ttataaccgt
     7621 tactatggcg ggaatattct tgatatggga ttacgtgata tccgcaatag ctggacggaa
     7681 tcggtatcag gcaataaaac cctgacgcgt tatcaggatt tgaaaacgcc ttataacgat
     7741 gaactggcaa tgggattgca gcagaaaatc ggtaagaacg ttattgcgcg cgcaaactat
     7801 gtttaccgtg aagcgcatga tcaaatcagc aaaagcagtc gtaccgacag cgcgactaaa
     7861 accaccatta ctgaatataa caacgacggc aaaacaaaaa tgcattcgtt caacctcagt
     7921 tttgaactgg ccgaacccct gcatatcagc caggtagata ttaacccgca aattgtcttt
     7981 agctatatca agagcaaggg caacttgtcg ttaaacaatg gttatgagga gagcaatacc
     8041 ggtgataacc aggtggttta taacggtaat ctggtttctt acgatagcgt tccagtggca
     8101 gattttaata acccattaaa gatctcctta aacatggatt tcacgcatca accgagcggg
     8161 ttagtgtggg cgaatacgct ggcctggcaa gaagcgcgta aagctcgcat tatccttggt
     8221 aagacaaatg cgcaatacat cagcgaatat tcagattaca agcagtatgt tgacgaaaaa
     8281 ctggatagca gcctgacctg ggacacccgc ttgtcctgga cgccacaatt tctgaaacaa
     8341 caaaacctga cgatcagtgc cgatattctc aatgtactgg atagcaaaac cgctgttgat
     8401 acaacgaata ccggtgtggc gacctacgcc agtggccgta ctttctggct tgatgtcagc
     8461 atgaaatttt aatagttcgg attttactta accgggtaaa ttcgcccggt tttcgcatgg
     8521 agataattat gagaaacctc tgtttcttac tgacgttagt ggcaactctg ttgctccccg
     8581 ggcggctgat tgccgccgcc ttaccgcagg atgaaaagtt aattaccggg caactggaca
     8641 atggcttgcg atatatgatt tatccgcatg ctcaaccaaa ggatcaggta aatttatggc
     8701 tgcaaattca taccggttca ttgcaggaag aagacaatga gcgcggcgtg gctcattttg
     8761 tagaacatat gatgtttaac ggcacaaaaa catggccggg taataaagtc atcgaaacat
     8821 ttgagtcaat gggcctgcgt tttggtcgcg atgttaatgc ctataccagc tatgacgaaa
     8881 cggtgtatca ggtgagtttg ccgactacgc agaaacaaaa tctgcaacaa gtgatggcaa
     8941 tcttcagtga atggagtaat gccgcaacct ttgaaaaact cgaagtagac gctgaacgtg
     9001 gcgtaattac tgaggaatgg cgtgcccatc aggatgcgaa atggcgcacc tctcaggcgc
     9061 gccgcccttt cctgctggct aatacccgta atttagaccg tgaacctatc ggcctgatgg
     9121 atactgtcgc cacggtcaca ccggcacaat tgcgccaatt ttatcaacgc tggtatcaac
     9181 caaataatat gacctttatc gtggtcggcg atatcgacag taaagaagcg ctggcgctga
     9241 taaaggataa tttaagtaag cttccggcta acaaagcagc tgaaaatcgc gtctggccga
     9301 caaaagccga aaaccacctg cgctttaata tcattaatga taaagaaaac cgggtgaacg
     9361 gcatcgcact ctattatcgc ctgccaatgg tacaagtgaa cgatgagcaa agctttatcg
     9421 aacaagctga atggagcatg ttagttcagc tgttcaatca acgtctgcag gaacgcatac
     9481 agtcgggcga gttgaagact atttctggcg gcactgcgcg cagcgttaaa attgcacccg
     9541 attatcagtc gctgtttttc cgtgtaaatg cacgagacga taatatgcag gatgctgcga
     9601 atgcattaat ggcagagttg gcaaccattg atcagcatgg tttttctgct gaagaactcg
     9661 atgatgtcaa atctacccgc ctcacctggc tgaaaaatgc ggttgatcag caagctgaac
     9721 gtgatttacg tatgctgacc agtcgcctgg catccagctc attaaataat acgccgttct
     9781 tgtcgccgga agagacatat caactttcga aacgtctgtg gcagcaaatt accgtgcaaa
     9841 gtctggcgga aaaatggcag cagttaagaa agaaccagga cgcattttgg gagcaaatgg
     9901 taaacaatga gcttgccgcc aaaaaagcat tgtctcctgc ggctatcctg gcgctggaaa
     9961 aagagtacgc caacaaaaag ctggcggctt acatcttccc aggcagaaat ttatcgttaa
    10021 cagtagacgc tgacccacag gcggaaatta gcagcaaaga aacgctggct gagaatctga
    10081 catcattaac actttccaat ggtgccaggg ttattctggc aaaatccgcg ggtgaagagc
    10141 aaaagctaca aattactgcc gtatcgaata aaggcgattt aagtttccct gcgcagcaaa
    10201 aatcacttat cgcgctggca aataaagcag taagcggaag cggcgttggt gagctctcct
    10261 cttccagcct gaaacgctgg agtgcggaaa attcggtaac catgagcagt aaagtcagtg
    10321 gcatgaatac gttgctctcc gttagcgcgc ggactaataa ccctgaacct ggttttcagt
    10381 tgattaacca gcgaatcacc cacagcacga ttaacgacaa tatttgggca tcgctacaaa
    10441 atgctcaaat tcaggcgttg aaaacgctcg accagcgtcc agcggagaaa ttcgcccagc
    10501 agatgtatga gacgcgctat gctgatgacc gcacgaaatt actgcaagaa aatcagattg
    10561 tacagtttac tgccgcagat gcgctggctg ccgatcgcca gttgttttca tctccagcgg
    10621 atatcacgtt tgtcattgtc ggtaatgtct cagaagacaa actcgtggcg ttaattacgc
    10681 gttacttagg atcaatcaaa cactctgatt cgccattagc cgcaggtaaa ccattaactc
    10741 gcgcgacgga caacgcatcg gttactgtaa aagaacaaaa tgaacctgtg gcacaggttt
    10801 cacagtggaa gcgttatgat tcccggacac ctgttaatct ggcgacgcgt atggcgctcg
    10861 atgcttttaa cgtcgcactg gcaaaagatc tacgtgttaa tattcgtgaa caggcatctg
    10921 gagcatacag cgtttcttct cgcctctcgg ttgatcctca ggccaaagat atcagtcatt
    10981 tgctggcttt tacttgtcaa ccagaacgac atgatgaact gttaacgtta gcgaatgaag
    11041 tgatggttaa gcgtctggct aaagggatca gtgagcaaga actgaatgaa taccagcaaa
    11101 acgttcagcg cagcctcgat atccaacagc gtagcgttca acaattagcg aacactattg
    11161 taaatagtct tattcaatat gacgatcctg cagcatggac tgagcaggag caattgttga
    11221 aacaaatgac ggtagagaat gttaacactg ccgttaaaca atatctttct catccggtaa
    11281 atacttatac cggagtatta ttgccaaaat aataacagcc ccgtcaacac atcgttggcg
    11341 gggattttag caatattcgc tatttttatg taataatttt ataaatgcgt tcaaaataat
    11401 aatcaagtac taatagtgat attttaaggt ctgattttta cgtgataatt caggagacac
    11461 agaatgcgca taaaaataac agcataaaac accttaccac cacccaagaa tttcatattg
    11521 cattgttttt caatgaaaaa atattattcg cgtaatatct cacgataaat aacattagga
    11581 ttttgttatt taaacacgag tcctttgcac ttgcttactt tatcgataaa tcctactttt
    11641 ttaatgcgat ccaatcattt taaggagttt aaaatggata agaagcaagt aacggattta
    11701 aggtcggaac tactcgattc acgttttggt gcgaagtcta tttccactat cgcagaatca
    11761 aaacgttttc cgctgcacga aatgcgcgac gatgtcgcat tccagattat caatgacgaa
    11821 ttatatcttg atggcaacgc tcgtcagaac ctggccactt tctgccagac ctgggacgac
    11881 gaaaatgtcc acaaattgat ggatttatcc attaa
//
LOCUS       DAZGXZ010000146        11880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000146 DAZGXZ010000000
VERSION     DAZGXZ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.146"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            21..584
                     /locus_tag="ACHKJF_004333"
     CDS             21..584
                     /locus_tag="ACHKJF_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951586.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            666..3311
                     /gene="yfcU"
                     /locus_tag="ACHKJF_004334"
     CDS             666..3311
                     /gene="yfcU"
                     /locus_tag="ACHKJF_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFP0951587.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTWPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGSVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDGGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GETRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDD
                     IKPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3331..4083
                     /locus_tag="ACHKJF_004335"
     CDS             3331..4083
                     /locus_tag="ACHKJF_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0951588.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4058..4606
                     /locus_tag="ACHKJF_004336"
     CDS             4058..4606
                     /locus_tag="ACHKJF_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951589.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAIDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4603..5073
                     /locus_tag="ACHKJF_004337"
     CDS             4603..5073
                     /locus_tag="ACHKJF_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951590.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5070..5594
                     /locus_tag="ACHKJF_004338"
     CDS             5070..5594
                     /locus_tag="ACHKJF_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951591.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5581..6453
                     /locus_tag="ACHKJF_004339"
     CDS             5581..6453
                     /locus_tag="ACHKJF_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP0951592.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6575..7126)
                     /gene="smrB"
                     /locus_tag="ACHKJF_004340"
     CDS             complement(6575..7126)
                     /gene="smrB"
                     /locus_tag="ACHKJF_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP0951593.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7292..8224
                     /gene="prmB"
                     /locus_tag="ACHKJF_004341"
     CDS             7292..8224
                     /gene="prmB"
                     /locus_tag="ACHKJF_004341"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0951594.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            8259..9344
                     /gene="aroC"
                     /locus_tag="ACHKJF_004342"
     CDS             8259..9344
                     /gene="aroC"
                     /locus_tag="ACHKJF_004342"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP0951595.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9348..10172
                     /gene="mepA"
                     /locus_tag="ACHKJF_004343"
     CDS             9348..10172
                     /gene="mepA"
                     /locus_tag="ACHKJF_004343"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP0951596.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10172..10981
                     /locus_tag="ACHKJF_004344"
     CDS             10172..10981
                     /locus_tag="ACHKJF_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP0951597.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10981..11529
                     /gene="epmC"
                     /locus_tag="ACHKJF_004345"
     CDS             10981..11529
                     /gene="epmC"
                     /locus_tag="ACHKJF_004345"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP0951598.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11563..11841
                     /locus_tag="ACHKJF_004346"
     CDS             11563..11841
                     /locus_tag="ACHKJF_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP0951599.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2943 a         2897 c         3102 g         2938 t
ORIGIN      
        1 ttatagttta aggacaaata atgagtaagt ttgttaaaac agctattgct gcggcaatgg
       61 tgatgggcgt tttcacctct acggcaacaa tcgctgcggg taacaacggt actgcacgtt
      121 tttacggcac cattgaagac tcagtgtgtt ctatcgttcc ggacgatcac aaactggaag
      181 ttgatatggg tgatatcggt gccgaaaaac tgaaaaataa cggcaccacc acgccgaaaa
      241 gtttccagat tcgtctgcaa gattgcgtat ttgatactca ggaaacaatg accactacct
      301 ttactggtac cgtttcttct gcaaatagcg gcaattatta caccattttc aataccgata
      361 ccggtgcggc atttaacaat gttagcctgg cgatcggtga ctctctgggt acctcttaca
      421 aaagcggcat gggtattgac cagaaaatag tgaaagacac ttctaccaat aaaggcaaag
      481 cgaagcagac actgaacttt aaagcctggc tggtcggcgc agctgatgcg ccagatctgg
      541 gtaatttcga agcaaatacc accttccaga ttacttacct gtaaggcata cccccatagc
      601 gacactgcta tgggggattt aaatggattt tgaccgaaca agaataaaaa cggatagaaa
      661 cgtgtatgcc tgaccattct ctttttcgat tacggattct tccgtggtgc attgcgctgg
      721 caatgtcagg gagttatagc agtgtctggg ctgaagacga cattcagttt gattcccgtt
      781 ttctggaatt aaaaggcgac acaaaaattg atctgaagcg tttttccagc cagggatatg
      841 ttgagcccgg aaaatacaat ttacaggttc aactaaataa acagccattg gcggaagagt
      901 acgatattta ctggtatgct ggtgaagatg acgcgagcaa aagctatgct tgtctgacac
      961 cggaactggt agcgcagttt ggtttaaaag aagacgtggc gaaaaatctt caatggagcc
     1021 acgatgctaa atgcctgaaa tccggtcaac tggaaggcat ggaaattaag gctgatttaa
     1081 gccagtccgc attagtcatt tcactaccgc aggcttacct cgagtatacc tggcccgact
     1141 gggatccgcc ttcacgttgg gatgacggca tttccgggat cgtcgcggac tacagcatca
     1201 acgcacaaac ccggcatgaa gaaaatggcg gtgatgatag taacgagatc agcggcaacg
     1261 ggacggtcgg ggttaacctg gggccgtggc gtatgcgtgc tgactggcag actaactatc
     1321 aacatactcg cagtaatgat gatgacgatg aattcagcgg cgatgaaact caaaaaaaat
     1381 gggagtggag tcgctactat gcctggcggg cgttaccatc attaaaagcc aaactggcgc
     1441 tgggcgagga ttacctcaga tccgatattt ttgatggctt taactatgtt ggtggcagtg
     1501 tcagtactga cgatcaaatg ttgcctccca atctgcgcgg ctacgcgcca gacatttccg
     1561 gcgtggcaca caccacagca aaagtgaccg tcagccagat ggggcgtgtg atttacgaaa
     1621 cgcaggttcc ggctggaccg tttcgtattc aggatcttgg tgattctgtc tccggtacgt
     1681 tgcatattcg cattgaagaa cagaacggcc aggtgcagga atatgacatc agcaccgcct
     1741 cgatgccata cctcactcgc ccaggtcagg ttcgctataa gatcatgatg ggccgtccgc
     1801 aagagtgggg acaccatgtc gagggtgaat ttttttctgg tgctgaagct tcctggggga
     1861 tcgctaacgg ctggtcgtta tatggcggcg cactgggaga tgaaaactat cagtctgcgg
     1921 cgcttggcgt cggtcgcgat ttgtctacat tcggctcggt cgcgtttgat gttactcact
     1981 cgcacaccaa actggataaa gacaccgctt atggcaaagg ttcgctggac ggtaactcct
     2041 tccgtgtgag ttattccaaa gactttgacc agctcaacag ccgcgttact tttgctggat
     2101 atcgcttctc ggaagagaac tttatgacca tgagcgagta tctggatgcc agtgacggcg
     2161 gaatggtacg cacgggcaac gacaaagaga tgtacaccgc cacttataac cagaacttcc
     2221 gcgatgcggg tgtttcggtt tatctcaact atacccgcca tacctactgg gatcgcgagg
     2281 agcagacaaa ctacaacatc atgctctcgc actatttcaa tatgggcagt attcgtaatg
     2341 tcagcatctc gatgactggc taccgttacg agtatgacaa ccaggccgac aaaggcatgt
     2401 acatttcgct cagtatgccg tggggcgaca acagtaccgt tagctataac ggtaactatg
     2461 gcagtgggac ggacagcagt caggtcggtt atttcagccg tgtcgatgac gcgactcact
     2521 atcagttgaa cgtcggcacc agtgacaaac acaccagcgt tgacggctat tacagccatg
     2581 atggttcgct ggcgcaggtt gacctcagtg cgaactacca tgaagggcaa tacacctctg
     2641 cgggcttgtc gttacagggc ggcgcaacgc ttactgccca cggtggcgca cttcaccgta
     2701 cccagaatat gggcgagaca cgcttgttga ttgatgccga tggcgttgcc gatgttccgg
     2761 tagaaggtaa cggtgctgct gtttatacca atatgtttgg taaagccgtc gtttctgacg
     2821 tcaataacta ttaccgcaat caggcgtata tcgacctcaa caaactgccg gaaaacgccg
     2881 aagcaaccca gtcggtggta caagccacgc taactgaagg ggcgattggc taccgcaaat
     2941 ttgccgtcat cagtggtcaa aaagcgatgg cggtgctgcg tttacaagac ggcagccatc
     3001 ctccgtttgg cgcagaagta aaaaatgata acgagcagac agtgggcctt gtcgatgatg
     3061 acggcaatgt ttatctggct ggggtgaaac ctggcgaaca catgagtgtg ttctggagtg
     3121 gtgttgcgca ttgcgatatc aacctgccgg acccgctgcc tgccgatctg tttaacggct
     3181 tgttactgcc atgccagcat aaaggcaacg tcgcgccggt tgttcctgat gacataaagc
     3241 ctgtcattca ggagcagacg caacaggtga cacccaccaa tccccctgtt tctgtttcag
     3301 ctaaccaata acgtgattaa ggaatgatcc atgtctgatt tactttgctc tgccaaattg
     3361 ggtgctacga cgctagcgtt actgcttagc gctgccagtt tgtctgccca ggcttctgta
     3421 acgccagatc gtacccgttt gatatttaat gaaagcgaca aatcaatcag cgtgacgttg
     3481 cgcaataacg atccgaaact gccttatctg gcccaaagct ggatcgaaga tgaaaagggt
     3541 aacaagatca gttcaccgtt aactgttctg ccaccggtgc aacgcatcga ttcaatgatg
     3601 aacgggcagg taaaagtgca gggaatgccg gatatcaaca aattgcctgc cgatcgtgaa
     3661 agcctgttct acttcaacgt gcgtgagatc ccgccaaaat caaacaaagc caacacgctg
     3721 caaatagcac tacaaacgcg tattaagttg ttctggcgac cgaaggcgct ggagaacgtc
     3781 agcatgaaaa acccctggca atataaggtt actttaaccc gcaacggtca ggaatttacc
     3841 gtcaataacc cgacaccgta ttacgtcatt ttcagtaatg ccagcacaca gaaaaatggc
     3901 aatccggcag ccggtttctc gccaatagtg atgtcgccga aaacctctgc accgctgaat
     3961 gtgaagatgg gtagcgtgcc ggtactgacc tatgtcaacg actatggcgc tcggatgccg
     4021 ctctttttca cttgcaacgg taacagttgc caggtcgatg aagagcagag ccgcaaaggc
     4081 tgaggccggg agagccagta tgaaaggatc ttgttctgtc tgggcggtaa ctgggttact
     4141 gatatgttgt gttggtagcg gctttgcagt tgcagcgagt aagtcagtaa gtatgaattt
     4201 acgggtgctt gtcgatgccc ctcctccttg tacggtaaat ggtgcggcag ttgagtttgg
     4261 taatgtattc attaataaga tcaatggggt tgattataaa cgtcccattg actactcgct
     4321 tgtttgtaac aatctggcaa tagatgacct gcgtctacag atgcaggcga caacggtcgt
     4381 gattaacggt gaaacagtta tcagcacggg gattcctggg tttggtattc gggttcagaa
     4441 aagtagtgat cacacaatac tcgacttaac ctctggctca tggttgccat ttaatttttc
     4501 ctcgggagtt cccgttctgg aagctgtgcc ggtgaaacag agtggtacaa cgttagcggc
     4561 agctgaattt aacgccagtg ccacaattgt ggtggattac caatgaaacg gatctcgctt
     4621 attttgctgt ggggcttttg ctcgatggca ctctcaaacg tctcttttca cggttattta
     4681 gtgcagccac ctaactgcac gattagcaac gctcaaacca ttgaaataac ttttcaggat
     4741 gtgcttattg atgacattaa tggcagcaat tatgagcaaa ccgtgccgta ttccattacc
     4801 tgcgatacgg cggttcgcga cccattaatg gaaatgacct tgagctggag tggcacacca
     4861 agcgattttg ataatgctgc agtaagcagc aatatcactg gtttaggcat tcagctcaaa
     4921 caggcagggc agagttttac gatcaatacg ccacttgtgg tgaatgaaac cgatttaccg
     4981 gtgttaacgg ctgtgccagt aaaaaagagt ggtgtcgttc tgccagaggc ggactttgag
     5041 gcctgggcca cgttacaggt agattaccaa tgaagaaaaa gcgtacgttg ttttttatta
     5101 gtagcctgat gttgctgggc agtggaacca caatagcagg agataatttg cactttaccg
     5161 gcaatttaat cagtaaatcg tgtacgccag tgattaatgg tagccagttg gcagaagtac
     5221 attttcctgc cattgccgcc agcgatttaa tgaaccttgg gcagtcagag cgagtgccat
     5281 tggtgtttca attgaaagat tgtcatagtt ctacattgtt taatgtcaaa gtaacgttga
     5341 caggaacaga agatagtgct ctacccggct ttctggcctt tgactcctcg tcatcagcat
     5401 ccggcgctgg gattggtatt gaaactgcgg caggcacatc ggtgccaatc aacaatacaa
     5461 caggcgtcac gctcccgctg aatcagggaa ataacagcct aaattttaat acctggttac
     5521 aggcaaagag cgggcgagat gtaacgtccg gagactttag tgccacagtg acggcaacct
     5581 ttgagtattt ctgaaatgaa aaaatggacc ataattttcg cgagcctaat gctgctcgtt
     5641 ctgagtgttg tcggcgcatc taagagttat gctggagata aacttatgag tgcgagtttt
     5701 gacagtgtga gagtttatta tgccatggac aaggtaaccg gtgctgtagc gagcagtgtt
     5761 ttcaatgtta cggtgataac gccaaaggag gttgcatatg ggaaatatga ttcttttgcg
     5821 tataaaggcg atacgttgcg ggttatttcc tggtctgggt cgggttcagc tccaactttg
     5881 gtactgactg actttgatac aattaataac tcaaattgcc ctggcataga tacgaaaatt
     5941 tttcgttgtg catatatgac gtttaaaatt accgttgcat ctgatgatta tggttgccct
     6001 tggatcgcat ccttttattc ctatacagat ttacccggat ttggaagcta tacggcccct
     6061 acagtgcata acacaatatg cccaacgata ccggtcgctt catacgatat ctcctggagt
     6121 gaaaattatg tcagccataa taaggcgtta cgaattcagt ccacggggtc aacagtgacc
     6181 accacgcttt cgacctactt gatggaaggc ggcagactct gtgatggcag taatttttct
     6241 gataatgatg gcagaggagc atactgccgc gcggtatcag aattgttaac ctttacctct
     6301 tacggttgtg ataaatcaac ggtaacggtt acgccaactc gtcatccggt aacggataaa
     6361 gtactacacg atattgtggt gaatgtgaat accagtagtg ggcaaccgat tgattcgacg
     6421 tgtcgttttc agtatgtatt gaatgaactg tagttcgtag tattgctctg atgcgtctta
     6481 tcaggcctac gaagtgaatt gcaatttatt gaattatcac atatttgtag gccggataag
     6541 gcgtttacgc tgctccggca tttgctgcca gcattcaggg caactccggc ggcaaccact
     6601 cttccacttc aatcaacacc aacaaagccg catcaccgcc gtactctttc ggtgcctggt
     6661 gaaatgccat cacatgcgga tgttgtgcca gccacagcgg tgtttgttgc ttcaaaatat
     6721 gcttcccgtg cccatgcatc acgcaggcac aaaacacatg ttcgcggcga caggcggcaa
     6781 tcaacgcgcc cagttcctgc ttggcctgca gctgcgtcag accgtgtaaa tccaaaaaca
     6841 attccggtga ataatcgccg cggcgcagtt tcttcgcctc aaaatggctg acatccgggc
     6901 gaacatattt caccggacct tcggtattta ataacggctg aaactcatcg gagaaataat
     6961 ggctggcatc agcctgctcc tggatcaagc gtttcaccgg cacttcgctg atttttttac
     7021 gctgcggtcg gtggacaatc gtgtcctgct taatcttgcg agtccccgcc atcaactggc
     7081 gaaacagagc ctggtcctcc tcgctaagtg ttgttttctt tttcatttat ctgtctctat
     7141 ttctaatttt cctacagttt acccgactcg tagcacaatc gatcgtttta aacgtatttt
     7201 tcctcactca ccgcgcgtga atgctgattt atcgtcatct tcatggcaaa ctagccgccg
     7261 aaattattgc gatcatgccc tggaggaata cgtggataaa attttcgttg atgaagcagt
     7321 aaatgagctg caaaccattc aggacatgtt gcgctggtcg gtaagccgct tcagcgcggc
     7381 aaatatctgg tacggtcacg gcaccgataa cccgtgggat gaagccgtac agctggtgtt
     7441 accatcgctc tacctgccgc tggatatccc ggaagatatg cgcaccgcgc gtctgacctc
     7501 cagcgaaaaa caccgtattg ttgaacgcgt gatccgccgc gtcaacgagc gcattccggt
     7561 ggcttacctg accaacaaag cctggttctg cggtcatgaa ttttacgtcg atgaacgcgt
     7621 gctggtgccg cgctcgccga ttggtgaact gatcaacaat aaatttgccg gactcatcag
     7681 caagcaaccg cagcatattt tagatatgtg taccggtagc ggctgcatcg ccattgcctg
     7741 tgcttatgcc ttcccggaag cggaagtcga cgcggtagat atctctcccg acgcgctggc
     7801 ggttgccgaa cagaacatcg aagaacacgg tctgatccac aacgtcattc cgattcgttc
     7861 cgatctgttc cgcgacttgc cgaaagtgca gtacgacctg attgtcacta acccgccgta
     7921 tgtcgatgcg gaagatatgt ccgacctgcc aaacgaatac cgtcacgagc cggaactagg
     7981 cctggcatct ggcaccgacg gcctgaaact gacgcgtcgc attctcggaa acgcggcaga
     8041 ttaccttgcg gatgatggcg tgttgatttg tgaagtcggc aacagcatgg tacatcttat
     8101 ggaacaatat ccggatgttc cgttcacctg gctggagttt gataacggcg gcgatggtgt
     8161 gtttatgctc accaaagagc aactgctggc ggcccgtgaa catttcgcga tttataaaga
     8221 ttaagtaaac acgcaaacac aacaataacg gagccgtgat ggctggaaac acaattggac
     8281 aactctttcg cgtaaccacc ttcggcgaat cgcacgggct ggcgctcggc tgcatcgtcg
     8341 atggtgttcc gccaggcatt ccgctcacgg aagcggacct gcaacatgac ctcgaccgtc
     8401 gtcgccctgg gacatcgcgc tataccaccc agcgccgcga gccggatcag gtcaaaattc
     8461 tctccggtgt ttttgaaggc gttactactg gcaccagcat tggcttgttg atcgaaaata
     8521 ccgaccagcg ttctcaggat tacagcgcaa ttaaagacgt tttccgtcca ggccatgccg
     8581 attacaccta cgaacaaaaa tacggtctgc gcgattatcg cggcggtgga cgttcttccg
     8641 cccgcgaaac cgccatgcgc gtggcggcag gggcgattgc caaaaaatat ctcgccgaga
     8701 aatttggtat tgaaatccgc ggctgcctga cccagatggg cgacattccg ctggaaatca
     8761 aagactggtc gctggttgaa caaaatccgt tcttctgccc ggaccccgac aaaatcgacg
     8821 cgttagatga actgatgcgc gcgctgaaaa aagagggcga ctccatcggc gcgaaagtca
     8881 ccgttgttgc tagtggcgtc cccgccggac ttggcgagcc ggtctttgac cgcctggatg
     8941 ccgacatcgc ccatgcgctg atgagcatca acgcggtgaa aggcgtggaa attggcgacg
     9001 gttttgatgt agtggcgctg cgcggcagcc agaaccgcga cgaaatcacc aaagacggtt
     9061 tccagagcaa ccatgcgggc ggcattctcg gcggtatcag cagcgggcag caaatcattg
     9121 cccatatggc gctgaaaccg acctccagca ttaccgtgcc ggggcggact attaaccgct
     9181 ttggcgaaga agttgagatg atcaccaaag gtcgtcacga tccctgtgtc gggatccgcg
     9241 cggtgccgat cgctgaagcg atgctggcga tcgttttaat ggatcacctg ctacggcagc
     9301 gggcgcaaaa tgccgatgtg aagactgata ttccacgctg gtaaaaaatg aataaaaccg
     9361 cgattgcgct gctggctctg cttgccagta gcgccagcct ggcagcgacg ccgtggcaaa
     9421 aaataaccca acctgtgccg ggtagcgcac aatcgatagg cagtttttct aatggctgta
     9481 tcgtcggcgc tgacacgctg ccgatacagt ccgaacatta tcaggtcatg cgtaccgatc
     9541 agcgtcgcta tttcggtcac ccggacctgg tgatgtttat ccagcgtctg agtagccagg
     9601 tgagcaatct gggcatgggt acggtgctga ttggcgatat ggggatgccc gctggagggc
     9661 gtttcaacgg cggtcatgcc agccaccaga ccggactgga tgtcgatatc tttctgcaac
     9721 tgccgaaaac tcgctggacc tccgcgcagc tcttgcgccc gcaagcactg gacttagttt
     9781 cccgcgacgg taaacacgtt gtctccacgc tgtggaagcc agaaattttc agcttgatca
     9841 aactcgccgc ccaggacaaa gacgtcacgc gcatttttgt taatccggcg attaaacaac
     9901 aactttgcct tgatgcgggc accgatcgcg actggttgcg caaagtgcga ccctggttcc
     9961 agcatcgcgc gcatatgcat gtacgattac gttgtcctgc cgatagtctg gagtgtgaag
    10021 atcaaccttt accgccacca ggcgatggtt gcggggcaga actgcaaagc tggtttgaac
    10081 ctccaaaacc gggaacaaca aagcctgaga agaagacacc gcctccgttg ccgccttcct
    10141 gccaggcgct actggatgag cacgtgatct aatggaaacg tttaatagcc tgtttatggt
    10201 ttccccgctg ttgctgggag ttctcttttt tgtcgccatg ctggcgggat ttatcgactc
    10261 gattgccggt ggcggtgggt tactcaccat tccggcattg atggctgcgg ggatgtctcc
    10321 cgctaatgcg ctggcaacca ataaactgca agcctgcggc ggctctattt ccgccactat
    10381 ctactttatt cgccgcaaag tggttagttt aagcgatcag aaactcaata tcgccatgac
    10441 ctttgtcggc tcaatgagcg gcgcactgct ggtgcaatac gttcaggctg atgtcttacg
    10501 gcagattttg cccattctgg taatttgtat cggcctctat ttcttgctga tgccaaagct
    10561 gggtgaagaa gatcgccagc gtcggatgta cggtttaccc ttcgcgttaa ttgccggtgg
    10621 ctgcgtcggt ttttatgacg gattctttgg tccggcagcc ggatcgtttt acgcactggc
    10681 ctttgttacg ctgtgcggat tcaacctcgc caaagccacg gcgcacgcca aattactcaa
    10741 cgcaacgtca aacatcggcg gtttactgct gtttattctc ggtggcaaag tgatttgggc
    10801 gacgggcttt gtcatgctgg tggggcagtt cctcggcgcg cgcatgggtt cacgactggt
    10861 gttgagcaaa ggacaaaagc tgatccgccc gatgattgtt atcgtctcgg cggtgatgag
    10921 tgccaaatta ctttacgata gccacggaca ggagatcctc cactggttgg ggatgaacta
    10981 atgaacagta cacaccacta tgagcagttg attgaaattt ttaatagctg ctttgccgat
    11041 gaatttaata cccgtctgat taaaggcgac gacgaaccga tctatcttcc tgctgatgcg
    11101 gaagtaccgt ataaccgaat tgtctttgcc cacggctttt atgccagcgc aattcatgag
    11161 atttcgcact ggtgtattgc cggaaaagcg cgccgtgagc tggttgattt cggctactgg
    11221 tattgcccgg atgggcgtga tgcccaaacg caaagccagt ttgaagatgt tgaagtgaag
    11281 ccgcaggcac tcgactggtt gttctgtgtg gcggcgggat atccgtttaa cgtcagttgc
    11341 gacaatctgg aaggcgactt cgaaccggat cgtgtggtgt ttcaacggcg ggtacatgcg
    11401 caggtgatgg attacctagc aaacggcatt cctgaacgcc cggcacgctt cattaaagca
    11461 ctacaaaatt attatcacac gccggaactt acggcggaac agttcccgtg gccggaagcg
    11521 ctcaactaaa gccagcgcca cgttttacaa tgaggaaaga agatgatcgc ggagtttgaa
    11581 tcacgcattc tggcattaat cgacggtatg gttgaccatg ccagtgatga tgagttgttt
    11641 gccagtgggt atttgcgtgg ccacctgaca ttagccatcg cagaactgga aagtggtgat
    11701 gaccactccg ctcaggcggt gcatacgacc gttagccaga gtctggaaaa agccattggt
    11761 gccggtgaat tgtcgccgcg tgaccaggcg ctggtgaccg atatgtggga aaacctgttt
    11821 cagcaggcgt cacagcagta agttgcggcg tctggagcac catgcctgat gcgacgctta
//
LOCUS       DAZGXZ010000147        11874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000147 DAZGXZ010000000
VERSION     DAZGXZ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.147"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..276)
                     /gene="ydhL"
                     /locus_tag="ACHKJF_004347"
     CDS             complement(37..276)
                     /gene="ydhL"
                     /locus_tag="ACHKJF_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP0951600.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPKQPSLF"
     gene            379..978
                     /gene="nemR"
                     /locus_tag="ACHKJF_004348"
     CDS             379..978
                     /gene="nemR"
                     /locus_tag="ACHKJF_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP0951601.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            1015..2112
                     /gene="nemA"
                     /locus_tag="ACHKJF_004349"
     CDS             1015..2112
                     /gene="nemA"
                     /locus_tag="ACHKJF_004349"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP0951602.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            2193..2600
                     /gene="gloA"
                     /locus_tag="ACHKJF_004350"
     CDS             2193..2600
                     /gene="gloA"
                     /locus_tag="ACHKJF_004350"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP0951603.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            2703..3350
                     /gene="rnt"
                     /locus_tag="ACHKJF_004351"
     CDS             2703..3350
                     /gene="rnt"
                     /locus_tag="ACHKJF_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP0951604.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            3443..8058
                     /gene="lhr"
                     /locus_tag="ACHKJF_004352"
                     /pseudo
     CDS             3443..8058
                     /gene="lhr"
                     /locus_tag="ACHKJF_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(8109..8456)
                     /gene="grxD"
                     /locus_tag="ACHKJF_004353"
     CDS             complement(8109..8456)
                     /gene="grxD"
                     /locus_tag="ACHKJF_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP0951605.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            8791..9606
                     /gene="mepH"
                     /locus_tag="ACHKJF_004354"
     CDS             8791..9606
                     /gene="mepH"
                     /locus_tag="ACHKJF_004354"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP0951606.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            9734..10315
                     /gene="sodB"
                     /locus_tag="ACHKJF_004355"
     CDS             9734..10315
                     /gene="sodB"
                     /locus_tag="ACHKJF_004355"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP0951607.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(10461..11630)
                     /gene="ydhP"
                     /locus_tag="ACHKJF_004356"
     CDS             complement(10461..11630)
                     /gene="ydhP"
                     /locus_tag="ACHKJF_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951608.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(11796..>11874)
                     /gene="cydH"
                     /locus_tag="ACHKJF_004357"
                     /gene_synonym="ynhF"
     CDS             complement(11796..>11874)
                     /gene="cydH"
                     /locus_tag="ACHKJF_004357"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP0951609.1"
                     /translation="LKFSLVTTIIVLGLIVAVGLTAALH"
BASE COUNT         2887 a         3189 c         3091 g         2707 t
ORIGIN      
        1 ttatcagata tgagaggagt atacgcaaga ttaggttcaa aagagtgatg gttgcttcgg
       61 ttcgtctgat gacgctggct tatttgcgcg taatttgcgc attaatcgct gccgacaaag
      121 gcgcagcacc tcttgttttt cgccatcgct cattttattc cagttaaaac gctcatcacg
      181 actacgaaaa cagccgcgac aaaacccgcg ttcgtcagac tggcaaattc cccggcacgg
      241 gctctggacg ggaaagaact ctaattgctc cgccacttcg ccctcctcag ataagattat
      301 taccattatt gaagctgtta atgtccaaag tagcaacttt gcttgcacta gaccgactag
      361 tctactacac tccaacgcat gaacaaacac accgaacatg atactcgcga acatctcctg
      421 gcgacgggcg agcaactttg cctgcaacgt ggattcaccg ggatggggct aagcgaatta
      481 ctaaaaaccg ctgaagtgcc gaaagggtcc ttctatcact actttcgctc taaagaagcg
      541 tttggcgttg ccatgcttga gcgtcattac gccgcatatc accagcgact gactgagttg
      601 ctgcaatccg gcgaaggtaa ctaccgcgac cgcatactgg cttattacca gcaaacactg
      661 aaccagtttt gccaacatgg aaccatcagt ggttgcctga cagtaaaact ctctgccgaa
      721 gtgtgcgatc tgtcagaaga tatgcgcagc gcgatggata aaggtgctcg cggcgtgatc
      781 gccctgctct ctcaggcgct ggaaaatggc cgtgagaacc attgtttaac cttttgtggc
      841 gaaccgctac aacaggcaca agtgctttac gcactgtggc ttggcgcgaa tctgcaggcc
      901 aaaatttcgc gcagtttcga gccactggaa aacgcgctgg cccatgtaaa aaacattatt
      961 gcgacgcctg ccgtttagca ggcatttttt atcaccagac gaccgggagc ctttatgtca
     1021 tctgaaaaac tgtattcccc actgaaagtg ggcgcgatca cggcggcaaa ccgtattttt
     1081 atggcaccgc tgacgcgtct gcgcagtatt gaaccgggtg acattcctac cccgttgatg
     1141 gcggaatact atcgccaacg tgccagtgcc ggtttgatta ttagtgaagc cacgcaaatt
     1201 tctgcccagg caaaaggata tgcaggtgcg cctggcatcc atagtccgga gcaaattgcc
     1261 gcatggaaaa aaatcaccgc tggcgttcat gctgaaaatg gtcatatggc cgtgcagctg
     1321 tggcacaccg gacgcatttc tcacgccagc ctgcaacctg gcggtcaggc accggtagcg
     1381 ccttcagcac ttagcgcggg aacacgtact tctctgcgcg atgaaaatgg tcaggcgatc
     1441 cgtgttgaaa catccatgcc gcgtgcgctt gaactggaag agattccagg tatcgtcaat
     1501 gatttccgtc aggccattgc taacgcgcgt gaagccggtt ttgatctggt agagctccac
     1561 tctgctcacg gttatttgct gcatcagttc ctttctcctt cttcaaacca tcgtaccgat
     1621 cagtacggcg gcagcgtgga aaatcgcgca cgtctggtac tggaagtggt cgatgccggg
     1681 attgaagaat ggggtgccga tcgcattggc attcgcgttt cgccaatcgg tactttccag
     1741 aacacggata acggcccgaa tgaagaagcc gatgcactgt atctgattga acaactgggt
     1801 aaacgcggca ttgcttatct gcatatgtca gaaccagatt gggcgggggg tgaaccgtat
     1861 actgatgcgt tccgcgaaaa agtacgcgcc cgtttccacg gtccgattat cggcgcaggt
     1921 gcatacacag tagaaaaagc tgaaacgctg atcggcaaag ggttaattga tgcggtggca
     1981 tttggtcgtg actggattgc gaacccggat ctggtcgccc gcttgcagcg caaagctgag
     2041 cttaacccac agcgtgccga aagtttctac ggtggcggcg cggaaggcta taccgattac
     2101 ccgacgttgt aatccaacat tgcgagcggc gtaaagccgc cgctatacta aaacaacatt
     2161 ttgaatctgt tagccatttt gaggataaaa agatgcgtct tcttcatacc atgctgcgcg
     2221 ttggcgatct gcaacgctcc atcgattttt ataccaaagt gctgggcatg aaactgctgc
     2281 gtaccagcga aaacccggaa tacaaatact cactggcgtt tgttggctac ggcccggaaa
     2341 ccgaagaagc ggtgattgaa ctgacctaca actggggcgt ggataaatac gaactcggca
     2401 ctgcttatgg tcacatcgcg cttagcgtag ataacgccgc tgaagcgtgc gaaaaaatcc
     2461 gtcaaaacgg gggtaacgtg acccgtgaag cgggtccggt aaaaggcggt actacggtta
     2521 tcgcgtttgt ggaagatccg gacggttaca aaattgagtt aatcgaagag aaagacgccg
     2581 gtcgcggtct gggcaactaa tctcctgccg ggcgtgaact catcgcgccc gcatctttac
     2641 tgcatcgaca agtaatattt gtcataatgc gcgctgcaat ttatccgtat taagagaatc
     2701 agatgtccga taacgctcaa cttaccggtc tgtgcgaccg ttttcgtggt ttttatcctg
     2761 ttgtgatcga tgttgaaaca gccggattta acgccaaaac cgatgcgctg cttgagattg
     2821 ccgccatcac cctgaaaatg gatgaacaag gctggctgat gccggacacc acattacatt
     2881 tccacgtcga accatttgtc ggcgcaaatt tgcagccaga ggccctcgcc ttcaacggca
     2941 ttgacccgaa cgatcccgat cgcggcgcgg tcagcgaata cgaggcgctg cacgaaattt
     3001 ttaaagttgt acgtaaaggt attaaagcga gcggctgtaa ccgcgccatt atggtggcgc
     3061 acaatgccaa ttttgatcac agctttatga tggccgccgc agaacgcgcc tcactgaaac
     3121 gtaacccgtt ccaccctttc gccacttttg acactgctgc actggccggg ctggcactcg
     3181 gacaaaccgt attgtcaaag gcttgccaga ccgctggcat ggacttcgac agcacccagg
     3241 cgcactccgc gctgtacgac accgaacgca ctgctgtgct gttttgtgaa atcgtcaacc
     3301 gctggaaacg tctgggaggc tggccgctac ctgccgccga agaggtgtaa tcgagtcgat
     3361 gcctgatgac atgcaatgat tcaggcatct atagtgaggc tattccacgc atcctgcatg
     3421 atattcacgg ggaatagcgt taatggcaga taatccagac ccttcatcgc tcctgccgga
     3481 cgtgttttca ccggcgaccc gcgactggtt tcttcgcgcc tttaaacagc cgaccgctgt
     3541 ccagtcgcaa acctggcatg tggcggcgcg aagcgaacat gcgctggtga ttgcaccgac
     3601 cggctccggg aaaacgctgg cagcatttct ctacgccctc gatcggctct tccgcgaagg
     3661 cggcgaagat acccgcgagg cgcataagcg taaaacctca cgcatcctct atatttcacc
     3721 gataaaagct ctgggcaccg acgttcagcg caacttgcag ctcccgttga agggtattgc
     3781 cgatgaacgg cggcggcgcg gcgaaacgga agtcaatctt cgcgtaggga tccgtactgg
     3841 cgatacgcct gcacaggaac gcagcaaact cacccgtaat ccgccggata ttctgatcac
     3901 cacacccgaa tcactctatc tgatgctgac ctcccgcgcg cgcgaaacgc tacgcggcgt
     3961 cgaaacggta attattgatg aagtccacgc ggtagcgggc agtaaacgtg gtgcgcatct
     4021 ggcgttaagt ctggagcggc tcgatgcgct gctccacacc tcagcacagc gaattggcct
     4081 ttctgccact gtgcgctcag ccagcgatgt ggcagcattt cttggtggcg atcgcccggt
     4141 tacggtagtc aacccgcccg caatgcgcca tccgcagata cgaattgtcg taccggtcgc
     4201 caatatggat gatgtctcat cggtcgccag cagcaccggc gaagacagcc atgccggccg
     4261 ggaaggctcc atctggccat atattgaaac gggtatcctt gatgaagtgt tgcgccatcg
     4321 ctcgaccatt gtctttacta attcacgtgg gctggcggaa aaactgacgg cacgattaaa
     4381 tgagctttac gccgcacgct tacagcgttc cccgtctatc gccgttgatg cggcccattt
     4441 cgagtcgacc tccggcgcaa cctctaaccg tgtacaaagt agcgacgttt ttattgcccg
     4501 ctcacaccac ggctccgtct ctaaagaaca acgagcaatc accgagcagg cgctgaaatc
     4561 gggtgagtta cgctgcgtgg tcgcaacctc cagtctcgaa ctggggattg atatgggcgc
     4621 agtggatctg gtgattcagg tggcaacgcc gctttctgtt gccagtgggt tacaacgcat
     4681 tggtcgcgcc ggacatcagg ttggcggtgt atctaaaggg ctgtttttcc ctcgtacccg
     4741 gcgtgactta gtcgattccg cagtcattgt agagtgtatg ttcgcaggca ggctggaaaa
     4801 cctgacaccg ccgcataatc cactcgacgt ccttgcgcag caaaccgttg ccgccgcggc
     4861 gatggatgca ttacaggtag acgaatggta ctcccgcgta cgccgtgccg caccgtggaa
     4921 agatctgcca agacgtgttt ttgacgccac gctggatatg ctttccgggc gctatccctc
     4981 tggcgatttt tctgcttttc gccccaaact ggtctggaac agggagaccg ggatattgac
     5041 cgcccgacct ggcgctcaat tgttggcggt taccagcggc ggcaccattc cggatcgtgg
     5101 catgtatagc gtgttattac ccgaaggtga agaacaggcc ggttcgcggc gggtgggtga
     5161 actggatgag gagatggtat atgagtcgcg ggtgaacgac attatcactc tcggcgctac
     5221 ctcatggcgg atccagcaaa tcacccgcga tcaggtgatt gtgactcctg ctccgggtcg
     5281 ttctgcccgg cccccttctg gcgtggtgaa ggtaacggac gtccggctga attaggcgag
     5341 atgatcggcg attttcttca tttactggcg gatggcgcgt tcttttccgg gactattccc
     5401 ccgtggctgg cagaagaaaa tacgaacgcc aatattcagg ggttgattga cgagcaacgc
     5461 aacgcgacgg gcatcgttcc ggggagtcgc catctggtcc tcgaacggtg ccgtgatgaa
     5521 attggcgact ggcgtattat tttgcactct ccctatggaa gacgggtgca tgaaccctgg
     5581 gcgctggcga ttgccgggcg aatacatgcc ctatggggcg ctgacgcgtc tgtggtcgcc
     5641 agtgatgacg gcattgttgc gcgcattcct gacactgatg gtaaattgcc cgacgccgcg
     5701 atttttttgt ttgaaccaga aaagttgctg caaattgtcc gcgaggcggt aggcagctcg
     5761 gcacttttcg ccgcccgttt tcgcgaatgc gccgcgcggg cattattaat gccggggcgc
     5821 actccgggcc atcgcacccc gctttggcaa caacggctgc gcgccagtca gttgctggaa
     5881 atcgctcagg gatatccgga ttttccggtc attctcgaaa ccctacgcga atgtctgcaa
     5941 gatgtttatg atcttcccgc actggaacgt ttgatgcgtc gcctgaacgg tggcgaaatt
     6001 caaatatccg atgtaacgac cactacgccc tcgcctttcg ccacaagttt attgttcggc
     6061 tatgtcgcgg aatttatgta ccagagcgac gccccgctgg cagagcgccg ggcatccgta
     6121 ctgtcgctgg acagcgagtt actgcgcaat ctactcggac aggtcgatcc gggggaatta
     6181 ctcgacccgc aggtcattcg ccaggtggaa gaagagttgc aacgactggc tcctggcaga
     6241 agagcgaaag gtgaagaagg attgttcgac ctgctgcgcg aactggggcc aatgaccgtt
     6301 gaagacctgg cgcaacggca tacaggcagc agtgaagagg ttgcgtcgta tctggaaaat
     6361 cttcttgcag taaaacgaat cttcccagcg atgattagcg gacaggagcg tcttgcctgt
     6421 atggatgatg ccgccaggct gcgtgatgcc ctcggcgtac gactaccaga gtcattgcca
     6481 gagatttatt tacatagagt cagttacccg cttcgcgacc tctttctgcg ctatctccgg
     6541 gctcatgctc tggtcacggc tgaacaactg gctcatgagt ttagtctcgg tattgccatt
     6601 gtcgaagagc agcttcagca actgcgtgaa cagggtctgg tgatgaatct gcaacaagac
     6661 atctgggtga gcgatgaagt atttcgtcgt ctgcgtttgc gctcgctgca agccgccaga
     6721 gaagcgacgc gtcccgttgc agccacgacc tatgcgcgat tgctgctgga acgtcagggc
     6781 gtattacccg ccaccgatgg tagcccggcg ctctttgcct caacatcgcc aggcgtttat
     6841 gagggcgtag atggcgtgat gcgggtgatc gaacagcttg ccggagtcgg tttacccgcc
     6901 tcactctggg aaagccagat cctgcctgcc cgcgtacgcg actattcgtc agaaatgctc
     6961 gatgaattac tggcaaccgg tgcagttatc tggtcggggc aaaaaaaact gggtgaagat
     7021 gacggcctgg tggcactgca tctacaggaa tatgctgcag aatcgttcac tcccgccgaa
     7081 gcggatcagg cgaatcgttc ggcgctgcaa caagcgatag tcgctgttct ggctgacgga
     7141 ggagcctggt ttgcacaaca aatcagccaa cggatacgcg ataaaatcgg cgaatcggtt
     7201 gatctctctg ccctgcaaga ggcgttatgg gcgctggtct ggcaaggcgt catcaccagc
     7261 gacatttggg caccgttacg cgccctcacc cgcagcagtt ccaacgcacg cacctcaact
     7321 cgccgcagtc accgggctcg tcgtggacgt cctgtctatg cgcaacccgt ctcgccgctg
     7381 gtatcttaca acacaccaaa tctggctgga cgctggtcgt tattgcaggt ggagccacta
     7441 aacgataccg aaaggatgtt ggcgctggcg gaaaatatgc tcgaccgcta cggcatcatc
     7501 agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat gcaaacgctt
     7561 tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga aggtctgggt
     7621 ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc gacacaagcc
     7681 acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc taatgtgtgg
     7741 ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg tcgggcgggt
     7801 gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg tggcaaaaaa
     7861 atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca cgcgctgact
     7921 accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa tgatctaccg
     7981 gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag ttcgccacaa
     8041 gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg tcagtattgc
     8101 aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt ttctttgatc
     8161 agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc gccgaccagc
     8221 tcgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg cagttccgca
     8281 cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca tgcggcaagc
     8341 gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc tttcatgtac
     8401 agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt gctcattgtc
     8461 ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca gtgcggcatc
     8521 ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat ttgttgcatt
     8581 tcaaatattc gtttcttctc tgatgcgcgg caaactggcg tatttataac catttttagc
     8641 tttcactgct gcattttttt gcgctcgcca acgaaacgta ttttttaaca ataaaagcta
     8701 ttaactttct cttcttctat gcattagaat catcaagttt tgtaaatcag acgcaggcat
     8761 gatagacctg ccttaacaga gggacgctca gtggcgcgga taaaccgtat ttcgatcacg
     8821 ctctgtgctt tactttttac caccctgcct ttaacgccta tggcccatgc ttcaaagcaa
     8881 gccagggaga gttctgctac cactcatatc accaaaaaag cagataaaaa gaaaagcacg
     8941 gcaaccacca aaaagaccca gaaaacagcg aaaaaagccg ccagtaaaag tacgaccaaa
     9001 agcaaaaccg cttcttccgt taaaaaatct tccattaccg cttctaaaaa cgccaaaact
     9061 cgcagcaaac acgccgtcaa taaaacggcc tcagccagct ttaccgaaaa gtgtaccaaa
     9121 cgtaagggct ataaatcgca ttgtgtgaaa gtcaaaaatg ccgcgtcagg aactcttgcc
     9181 gacgcgcaca aagcgaaggt gcaaaaagct acgaaagtgg caatgaataa actgatgcag
     9241 caaattggta agccatatcg ttggggtggc agctcaccgc gtaccggttt tgattgcagc
     9301 ggcctggttt attacgctta taaagatttg gtgaaaattc gtattccgcg tacggcgaat
     9361 gaaatgtatc acctgcgtga tgcagcgcca atcgaacgta gtgaactgaa aaacggcgac
     9421 ctggtctttt tccgtactca gggacgcggc acagccgatc atgtcggcgt gtatgtcggc
     9481 aacggcaaat ttattcagtc accgcgcaca ggtcaggaaa ttcaaatcac ttctctcagt
     9541 gaagactact ggcagcgcca ctatgttggc gctcgtcggg taatgacccc aaaaacactt
     9601 cgctaaaact ttaccctgtt gttacggcaa cagggtaagt tcatcttttg tctcaccttt
     9661 taatttgcta ccctatccat acgcacaata aggctattgt acgtatgcaa attaataata
     9721 aaggagagta gcaatgtcat tcgaattacc tgcactacca tatgctaaag atgctctggc
     9781 accgcacatt tctgcggaaa ctatcgagta tcactacggc aagcatcatc agacttatgt
     9841 cactaacctg aacaacctga ttaaaggtac cgcgtttgaa ggtaaatcac tggaagagat
     9901 tattcgcagc tctgaaggtg gcgtattcaa caacgcagct caggtctgga accatacttt
     9961 ctactggaac tgcctggcac cgaacgccgg tggcgaaccg actggaaaag tcgctgaagc
    10021 tatcgccgca tcttttggca gctttgccga tttcaaagcg cagtttactg atgcagcgat
    10081 caaaaacttt ggttctggct ggacctggct ggtgaaaaac agcgatggca aactggctat
    10141 cgtttcaacc tctaacgcgg gtactccgct gaccaccgat gcgactccgc tgctgaccgt
    10201 tgatgtctgg gaacacgctt attacatcga ctatcgcaat gcacgtcctg gctatctgga
    10261 gcacttctgg gcgctggtga actgggaatt cgtagcgaaa aatctcgctg cataataact
    10321 gatggcaaat gcagcattgc ctgatgcgct tcgcttacca tgccaacgcg gatgaggcgt
    10381 taacagcgca ttcggcaaga taaatcgcac gttgtcagca actgtaacgc agaaggttat
    10441 ccttctgcgt ttttgtttaa ttagctgtta gcaacgcaaa ctgtttcagg ttgttttctg
    10501 gctgacataa acaccagcaa taatgccagt cccgcgacaa tcgctcccat caccggcaca
    10561 aagctgtatc ccagcccagc ggaaattacc gcaccaccag cagctgctcc cagcgcattt
    10621 ccaagattaa aggcaccaat attgactgat gaagacagac ctggcgcttc actggcgaca
    10681 cgcatcacgc gcatctgtaa cggcggtacg accgcaaagg ttgctgcgcc ccacaccacc
    10741 atgctaatag ctgcgccgaa ctcattgcgg gccaggaacg ggattgccag cataatcacc
    10801 atcaacaaca acaaaaagcc tttcaacgtg ccgttaactg aacgatctgc cagtttgccg
    10861 ccgagatagt taccgataga gaatccgaca ccaatcagca ccagcattgc cgtgacgaac
    10921 actggtgttg cgtgggtaat actttgtagt accggagaga tataggtgta gagagtaaac
    10981 attgcaccag ctcccagtac cgtcgtcagc aatgcagaca gcacctgcgg acgcattaat
    11041 accgccagct cttttttcac ttcaggtcgt gcccctgcac cacctttagg taatgagaag
    11101 aacagactta ccattgaaat cactcccagc cccgccgttg ccagaaatga catccgccag
    11161 ccgatggttt cacccaacca ggtcgccgcc ggcacgccac cgatatttgc cagggttaac
    11221 cccataaaca tagtggcaac tgcgctggcc tgtttatgtt ttggcaccac gcttgcggcc
    11281 acgactgaac ccaaaccaaa aaatgctccg tgattcaggc tggtcaaaat gcgtgaaagc
    11341 atcagggtca tataatccgg cgcgatggcg gaaagtacgt tgccgagcgt gaaaattgcc
    11401 atcaggaaaa tcaacgcact gcggcgggca cgatgagaaa gtagaagcgt catcagcggc
    11461 gcgccaacca ttacgccaac tgcataggca ctgattaaca ttccggcagc gggaatcgag
    11521 acatccacac cgcgcgcaat gacgggcaac aaacccattg gcgagaactc cgttgtcccg
    11581 ataccaaacg cgccaatcgc cagcgccagc aacggatagt taattttcat gccttatctc
    11641 cacctcttcg cgtcattacg cgatattcat taaagtggcg aaagcatgac agcaatcaca
    11701 aaaaaatgaa aataacaaaa agagaaaaca cttttgccat tttgctaaca aacaggaagg
    11761 agatgcgagg gagaacgcgc tccctcgaga ggaaatcagt gcagcgcggc agtcaaaccc
    11821 acggccacga tcaaaccgag gacgataatc gttgttacca gtgaaaattt aagg
//
LOCUS       DAZGXZ010000148        11864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000148 DAZGXZ010000000
VERSION     DAZGXZ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.148"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1143
                     /gene="ompC"
                     /locus_tag="ACHKJF_004358"
     CDS             <1..1143
                     /gene="ompC"
                     /locus_tag="ACHKJF_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0951610.1"
                     /translation="MLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTFSDNPGDD
                     GDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLKFGEMGSF
                     DYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFGLVEGLNF
                     ALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGAAYSNSDR
                     TNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMTPYGNVGI
                     ANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIG
                     ATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
     gene            complement(1177..1587)
                     /locus_tag="ACHKJF_004359"
     CDS             complement(1177..1587)
                     /locus_tag="ACHKJF_004359"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951611.1"
                     /translation="MLNIIHAKFSLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACVADGDEMPIKKPEFDEGRTQASV"
     gene            1734..2585
                     /gene="hchA"
                     /locus_tag="ACHKJF_004360"
     CDS             1734..2585
                     /gene="hchA"
                     /locus_tag="ACHKJF_004360"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP0951612.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(2693..4051)
                     /gene="hprS"
                     /locus_tag="ACHKJF_004361"
     CDS             complement(2693..4051)
                     /gene="hprS"
                     /locus_tag="ACHKJF_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP0951613.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(4051..4722)
                     /gene="hprR"
                     /locus_tag="ACHKJF_004362"
     CDS             complement(4051..4722)
                     /gene="hprR"
                     /locus_tag="ACHKJF_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP0951614.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4855..5268
                     /gene="hiuH"
                     /locus_tag="ACHKJF_004363"
     CDS             4855..5268
                     /gene="hiuH"
                     /locus_tag="ACHKJF_004363"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP0951615.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5377..6381
                     /gene="msrP"
                     /locus_tag="ACHKJF_004364"
     CDS             5377..6381
                     /gene="msrP"
                     /locus_tag="ACHKJF_004364"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP0951616.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6382..7017
                     /gene="msrQ"
                     /locus_tag="ACHKJF_004365"
     CDS             6382..7017
                     /gene="msrQ"
                     /locus_tag="ACHKJF_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP0951617.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFALATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            7279..7929
                     /gene="zinT"
                     /locus_tag="ACHKJF_004366"
     CDS             7279..7929
                     /gene="zinT"
                     /locus_tag="ACHKJF_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP0951618.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            8272..8802
                     /gene="yodB"
                     /locus_tag="ACHKJF_004367"
     CDS             8272..8802
                     /gene="yodB"
                     /locus_tag="ACHKJF_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0951619.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAVLFHH
                     YIQKDNTLLRMMPRRK"
     gene            9642..9974
                     /locus_tag="ACHKJF_004368"
                     /pseudo
     CDS             9642..9974
                     /locus_tag="ACHKJF_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332514.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(10265..10738)
                     /locus_tag="ACHKJF_004369"
     CDS             complement(10265..10738)
                     /locus_tag="ACHKJF_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951620.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRVILAREFFIPENRKSVPTHD
                     ESWVIICDAMVSRLMRCMFQGRRYLQLDAEEITGRLETDRKIRNGDWEQNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGIHTKTVNTFLYMGLGKNGLRYRSVKHLVGRA"
     gene            complement(10826..>11864)
                     /locus_tag="ACHKJF_004370"
     CDS             complement(10826..>11864)
                     /locus_tag="ACHKJF_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0951621.1"
                     /translation="SAIDSFYAANPKINKDDPNIKQMVAQAAGNSGRYTVSLPSGLPS
                     LTTENIFNLKVTDIEKIKSNIDGVNSVITSKTAKDYNIAISNGMSSEAALTAAKDGNG
                     ALLQEFNRLGTNVNDLKNATTFALDENGEITYEKGVESIPVKDVVAEVKADTTIRKNK
                     DGSYTLDQTAPDTVRVNAAIVDLDKNLKDRTTVGVNSDGSLTTAEGATKTVAVSEGLV
                     SLSGRTGRIDAAVGGIDTRVTKNTQTIQNHSRQLQEHNARLNSQQRQIRENHEEMKRA
                     AAQSAALAGLFQPYSVGKFNATAALGGYSDKQAMAVGVGYRFNEQTAAKAGIAASDGD
                     VSYNVGVNFEF"
BASE COUNT         3315 a         2782 c         2436 g         3331 t
ORIGIN      
        1 atgctggtcc cggcgttatt agttgctggc gcagcaaatg cggctgaaat ttataataaa
       61 aatggcaata aactggatct gtacggaaaa gttgatgctc gtcatacttt ttccgataac
      121 cctggcgatg acggcgatga aactatcatc gagttaggct tcaaaggtga gacccaaatt
      181 acagatcagt taactggtta tggtcaagca ctgaccaaaa caaaggctag cgacactgaa
      241 ggttctgata atacttatgt caaacttgct tttgcaggtc tgaagttcgg cgagatgggc
      301 tcctttgact atggtcgtaa ctatggtgtg atttacgacg ttgaagcctg gacagatatg
      361 cttcctgtat tcggcggcga ctcctatacc tggactgata acttcatggc aggccgtgca
      421 aatggggtag ctacttaccg caatagtgat ttcttcggtc tggtggaagg tctgaacttt
      481 gcattgcagt atcaaggtaa caacgaaggc agcaatgcag gagaagatca agaaggtact
      541 aaaaatggtc acgaagacgt tcgcttccag aacggtgatg gttttggtct ttccacttca
      601 tatgactttg acttcggttt gagcttaggt gccgcttact caaactctga ccgtaccaat
      661 tctcaagtag ctcttggcgg gtatcactac aatgaataca gcaaatttgc tggtggtgat
      721 acagctgaag catggacatt tggtgcaaaa tacgacgcca acaatgttta tcttgcaatg
      781 atgtatgctg aaacccgcaa tatgacacca tatggtaatg tgggtatcgc aaataaaacc
      841 cagaactttg aagctgtagc acagtatcag ttcgattttg gtctgcgccc atctcttgca
      901 tatgtttatt caaaaggcaa agacctcggc ggtaacgact ataacaacaa tggtcatcaa
      961 gagtatgttg atcaggatct ggtcaactat gttgaaattg gagcgaccta ctatttcaat
     1021 aagaacttct caacatatgt tgactataaa ataaacttgt tggataaaga tgacgatttc
     1081 tatgacaaca atggcatcgc aacagatgac gttgttggtg tgggcttagt ctaccagttc
     1141 taatcagcca acgtgctgcg gcgtgttact gtaaaactaa acactagcct gagttcgtcc
     1201 ctcatcgaat tcaggctttt ttattggcat ttcatctcca tctgcgacac acgcaattgc
     1261 caatgcatat cgatacagca ccttaccctg cccctcctgg cacaataaca tcaaccagcg
     1321 actcttcaaa ccaacaccca aatgcatttc tttatgaatt aatccgtaac aaacacgctt
     1381 cccatataga aatatttgca aaaatagtgc atgaaataat cttttctcta ccaatccgcg
     1441 atataaatat tcatcagtac cacaatattt cacagcacta aatctctccc cgccgccccg
     1501 tacctttgat aatggtctaa aatcattgag gccacttgcg acgacgttgc tcaataagga
     1561 aaatttggcg tgaataatat tcaacaccag tgcagcatca tcattacata cttacacccg
     1621 ccgactaccg ctttcgatat attgactcaa ttttccagac tggttaaaaa tagcgaccag
     1681 ctcagtcgca aatatagtga ctaccctaac taagcaacaa taaggaatac actatgactg
     1741 ttcaaacaag taaaaatccg caggtcgata ttgctgaaga taatgcattc ttcccttcag
     1801 aatattcgct tagccaatat accagtcccg tctctgatct tgatggcgtg gactatccaa
     1861 aaccgtatcg cggtaagcat aaaattctgg tgatcgccgc ggacgaacgt tatttgccaa
     1921 ccgataacgg aaaactgttc tcgaccggta accatccgat tgaaacgttg ctgccgttgt
     1981 atcatctcca tgctgcaggt ttcgaattcg aagtggcgac catttccggt ctgatgacca
     2041 agtttgaata ctgggctatg ccgcacaaag atgaaaaagt aatgccattc tttgagcagc
     2101 ataaatcgtt gttccgcaat ccgaagaaac tcgcggatgt tgtcgccagc ctcaacgctg
     2161 atagcgaata tgcagcaatc tttgttcctg gtggtcatgg cgcacttatt ggtttaccag
     2221 aaagccagga cgtagctgct gctttgcagt gggcaatcaa aaatgaccgt tttgttatct
     2281 ccctttgcca cggcccggcg gcttttctgg cgcttcgcca cggcgataac ccactgaatg
     2341 gttattccat ttgcgcattc ccagacgccg cagacaaaca aacgccagag ataggctata
     2401 tgccgggtca tctcacctgg tacttcggcg aagagctgaa gaaaatgggc atgaacatta
     2461 ttaatgacga catcaccggg cgagtacata aggaccgtaa agttctcacc ggcgacagtc
     2521 ctttcgcagc gaatgcgtta ggtaaactgg cggcgcagga aatgctggca gcttatgcgg
     2581 attaatccct gttacgtttg aatgtaaacg cataatcaat cgcagccacg ttaatactca
     2641 cgacgtggct gcgttttttc atggttgatt tatggcgtac tgaagcccta tgttaatttc
     2701 tttgcggtaa cataatccgg aacacattat gcttattgag atagtgatac gtagcacttc
     2761 ccccatgtaa ttcggcaatc gctttgacta aagaaagacc aagtccctga cctacggaat
     2821 gacgcgaatt atctccccgc caaaatctac ggaagagttt ttcaggctca tgaattttcg
     2881 ttccaggact ggcgacatca atattaagat agccgttggt atcaagaaaa ctggttatat
     2941 gaatacgcga tttttctggt gaatatctaa tggcattaac aataagattc gataacattc
     3001 gttgcagtaa aattttatcc gcaaagattt gctgattgca ctcgacctta aagcaaatct
     3061 ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt ttcgacttcc ttattgagag
     3121 aaagcgagtc cagtttcacc aaaacattgt ttttatctgc ccgggcaaga aacagtatgt
     3181 tctctgttaa ccgcgaaata ttttccagct cttcaatgtt accggcaatt gttttttgat
     3241 attcagcgat acttctggtt tgactgagcg taacctgatt ctgacccagt aatgcattaa
     3301 tgggcgttct aagttcatga gcgagatcgt cagcaaattg acttagacgc tcaaaatctt
     3361 tgactaaagc atgatgcatt ttattcaacg cctgcccaag aggttttagt tctctcggta
     3421 atgcgctaac ctcaacaggc tcccggctat cgttataatt cagcgcttcc gttacaccac
     3481 tcaacttttt gatctctcgt aatcccgttc tgattaacag cggacttaat actgagcaaa
     3541 gtacaatggc gacaatgcaa attataatgc tattaatttt atactgttca agcatgttat
     3601 gtctggctga agccaattta gccacagtaa ccgttaatga tggcgaaact tcatcaatat
     3661 taattcgtaa agcatcaatc tcagtatcat taataatgct tctgtaaatg ccagctgcgc
     3721 tgattgtctc actagcaggt atgttattta acatgtcatc actgacattt gtccggttaa
     3781 caattttatt gatgccatca ccatgaatga tcaagatatc ctgactaaca tccatcatcc
     3841 ggttaaagta tacaggtaac gtatctggat ttaccccatc aattaacaac tgcttgatct
     3901 gtgctgtccg gttaatgagt gttgtatcat cgcgccattt caactcactt gccaggccat
     3961 tatagagagt ccagacaatt ccagcgccag caacagacag cagcaatata aaaagcaagg
     4021 ttaaacggac ggttatagat aatcttttca ttattttttt accgctacga atgaatagcc
     4081 catcccccgg attgtggcga ttagcttttc aggaaaagga tcatcaactt ttgcgcggag
     4141 cctgcgaatg gcgacatcca ccgtattggt atcactatca aagttgattc cccaaatttc
     4201 actcgcaata accgttctgg gtataatttc gccagctctg gaggccagta gccaaagtaa
     4261 ctgaaactcc ttgcgcgtca gtgtaatact gatattgtcc ctgctcacac tttgacttac
     4321 agagtccatt cttaagccac tgatttctaa tgttgaattc aaagcgtgat gttgccttaa
     4381 ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa ggttttacca gataatcatt
     4441 tgctccactg tccagccctc tgactctgtc atcgacagaa tcccttgcag taaggcaaat
     4501 aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc tgccagccat ccatacccgg
     4561 aagcataata tccagaatga tcaatgcata atcatccttc agcgcaagat aaagcccatc
     4621 tctgccatca gaaacggcat caatgacata acccgcttcg gaaagcccct gcgttaccca
     4681 ctcctgggtc ctttgattat cttcaataag taaaatcttc atgaaaataa tatgccataa
     4741 atcatatgtt atagaaacag cctggttcat tacaaaattg taatgctgct gtaaggttac
     4801 cctggccgct ttttcgctat cctcaaaact cattcacatg acaaggatat aaagatgtta
     4861 aagcgttatt tagtactctc cgtagtaacg gcagcatttt cattaccttc tttggtttat
     4921 gccgcacaac aaaacattct tagcgtgcac attttgaacc agcaaaccgg aaaacccgct
     4981 gccgacgtga cagtcactct tgaaaagaaa gcggacaacg gctggttaca acttaatacc
     5041 gccaaaacag ataaggatgg acgaattaag gcactgtggc ccgagcaaac tgcaactacg
     5101 ggcgattacc gtgtcgtatt taaaaccggg gactatttca agaaacaaaa tcttgaaagt
     5161 ttcttccctg agatccccgt tgaatttcat attaataaag tgaacgagca ttatcatgtg
     5221 cctttattac ttagccaata tgggtattca acctatcgtg gcagttaatg cataaatatg
     5281 taaaagcaga tctctgctac tgttagggag gacactccct gacagattaa cagtaaacgg
     5341 ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga aaaagaatca atttttaaaa
     5401 gaatcagatg ttacggccga gtcggtattc tttatgaagc gtcgacaggt gttaaaagca
     5461 ctgggcatca gcgcagctgc actttctttg cctcacgctg cgcatgccga tctgcttagc
     5521 tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa aaccgctgga gttcagcaag
     5581 cctaccgcct ggcaaaataa tctgccactg acgccagtag ataaagtctc cggttataac
     5641 aacttctatg aattcgggct ggataaagcc gatcccgccg ctaatgctgg tagcctgaaa
     5701 accgatccat ggacactgaa aatcagcggc gaagtggcaa agccattgac cctcgatcac
     5761 gatgatttaa cccgtcgctt cccgctggaa gagcgtattt atcgtatgcg ctgcgtggag
     5821 gcatggtcga tggtggtgcc gtggattggt tttccgctgc acaaattgct ggcgcttgcc
     5881 gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa tttatgcacc ggaacaaatg
     5941 ccaggccagc aggaccgctt tatcggcggc gggctgaaat atccttatgt cgaaggattg
     6001 cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg tgggtgttta tggcaaggcg
     6061 ttaccgccac aaaatggcgc gccggtacga ctgattgtgc cgtggaaata tggctttaaa
     6121 gggattaaat cgatagtcag tattaagctg acccgcgagc gtccgccaac cacctggaat
     6181 ctggcagcgc ctgacgaata cggtttttac gccaacgtta atccgcatgt tgatcacccg
     6241 cgctggtcac aggctaccga acgatttatt ggttcaggcg gcatcctcga tgttcagcgc
     6301 cagccaacgc tactgtttaa tggttacgcc gaccaggtgg catcgctgta tcgtggcctg
     6361 gatttgcggg agaatttctg aatgcgtctg acagcaaaac aggtgacatg gctgaaagtt
     6421 agcctgcatc ttgccggatt gttgccgttt ctctggctgg tctgggcgat caatcacggt
     6481 ggactgggtg ccgatccggt gaaagatatt cagcatttta ccggtcgcac tgcactgaaa
     6541 tttttgctgg cgaccttgtt aatcacacct ctggcacgct acgcaaaaca gccgttattg
     6601 atacgcactc gccgcctgtt aggcttatgg tgctttgcct tggcgacact gcatttaacc
     6661 agttacgcat tgctggagct gggcgtgaat aatctggcgt tattaggcaa ggagttaatt
     6721 acccggcctt atttaacgtt aggaattatc agctgggtta ttttgcttgc tttagcgttt
     6781 acttcaaccc aggcgatgca gcgaaaactc ggcaagcact ggcaacagtt gcataatttc
     6841 gtctatcttg tcgcgatcct ggccccaata cattatctgt ggtcagtgaa gattatttca
     6901 ccgcagccgc tcatctacgc tgggctggct gtactgcttt tagccttacg gtataagaag
     6961 ttgcgttccc tttttaaccg gttacgcaag caggttcaca acaaattatc tgtttaactt
     7021 catattgccg acaaagtacg cgttatccct gccggatgtg gcgcgagcgc cctgtccggc
     7081 ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa cctcaaatta tcaacataat
     7141 tcatttcatt tcatttcaat aagatagata agtacacctg agaaaactat gctctcatct
     7201 cctaagagtt gttgcatttt gccatatgtt acaatataac attacacatc atatacaatg
     7261 actctggagg aaactgtttt ggcaattcgt cttcacaaac tggctgttgc tttaggtgtc
     7321 tttattgtta gcgctcctgc cttttcgcat ggtcatcact cacacggcaa acccttaaca
     7381 gaggtcgaac aaaaagctgc taatggtgtt tttgatgatg ccaatgtaca aaaccgaacg
     7441 ctcagtgact gggatggagt ctggcaatcc gtttatcctt tactgcaaag tggcaaactt
     7501 gaccccgtct ttcagaagaa agcggatgca gataaaacta aaacatttgc tgaaattaaa
     7561 gattattatc acaaaggtta tgcaacagat atcgagatga ttggcattga ggacggcatt
     7621 gttgaattcc atagaaataa tgaaacaaca tcctgtaaat atgattacga tggatacaaa
     7681 atactcacct ataaatcagg caagaaaggc gttcgttact tattcgaatg taaagatcct
     7741 gaaagcaaag cccctaaata tatacaattt agcgatcata taattgcacc acgaaaatca
     7801 tctcattttc acatttttat gggtaatgat tcacagcaat ctttattaaa tgaaatggaa
     7861 aactggccaa cgtattatcc atatcagttg agtagcgaag aagtggtcga ggaaatgatg
     7921 tctcattgag tattctcatg ataacgcctc gatgccgctt tagtaagtca tcataactgc
     7981 cactggtcca tccacaaacg ccactgaacg caagctagct acagacacgc tcatcactat
     8041 gacgtgtctg tatattaata agctaacccg cattgagtta accaataacg gattccatac
     8101 acaatacggc caacagagaa agatacctgt gctcacgcca ttgcttatat tggctggtta
     8161 caatgtgcac tatcaatttt ttaaataaca aattcttaaa accacatctg tataaggaat
     8221 ttttaaggtt cgtgggatag cttgactgta aaaattacag gagctacaaa aatgaaccga
     8281 ttctcaaaaa ctcaaattta tttacattgg ataacgctgc ttttcgttgc aataacctat
     8341 gccgcgatgg aactccgtgg ctggtttcct aaaggtagta gtacttatct gctgatgcga
     8401 gaaacacatt acaatgcggg tatattcgtt tgggtgttaa tgttttcacg cctgattata
     8461 aaacaccgtt atagtgatcc ttctattgtg ccaccgccac ctgcctggca aatgaaagcg
     8521 gcttcgctaa tgcacatcat gctttatata accttccttg cacttcctct gctggggatt
     8581 gctttgatgg cttacagtgg aaaatcgtgg agtttccttg gtttcaatgt gtctcccttt
     8641 gttaccccaa acagcgaaat taaagcactg ataaaaaata ttcacgaaac ctgggcaaat
     8701 ataggttact ttttaatcgc agctcacgct ggcgcagtac tctttcatca ctacattcag
     8761 aaagataata ctctgttacg aatgatgcct cgccgcaaat aagtcgatcc cctgacaagg
     8821 aaatgtgaac catggacatc aacgcgtcat aaatgtttac gcaagtgcac aatgcaccct
     8881 ttccactatc aacaaggagg agcagaaact gtgtaaatcg tacaatggta agacttatca
     8941 aaaatacagg ttaattttgg catttaaacc ttttttactt gttagtatga tacaaaggct
     9001 ttcaaaaaag ctgggaaatt caacaactct tggaaattat ggactttgtc ccgcggcttt
     9061 aatctgacgg ccgcgttcct tttttgactt aattattgac cccttctcta cgcaacttca
     9121 gttcccacca ccaactttgc ggcagtttca taggatgaat gtctaaaaga ataatggtga
     9181 gcgataagaa aacgactgaa taactgcaca ttttcgctcg aaagcttcct gtcagatcca
     9241 tagcgaatca agtgctgaat gtcacagtat cgaacagaaa acagtgacga tctaaccctt
     9301 caagaatatt ctacgattgt tctgtttagg aaaagcaagg cgggaagtcg ggagataagt
     9361 cattgataaa gtggcggaga gagggggatt tgaacccccg gtagagttgc ccctactccg
     9421 gttttcgaga ccgataatac cgaatcgtaa aatcaaaagg ttataaattt ttcttggaat
     9481 aaatgtatta aaaacataca cgaaaaatca ataaattacg tgaaatttaa gaagaatatt
     9541 ccaagctttc tttggggcat ttttgaccat cttacttttt tctttctgat aaaatattgt
     9601 ctgttatcca ttatcattac taatacttgc gtagtgaatt tatgaacaat cctaatccat
     9661 gcgtgatgtg tggtgcctgt tgtgcatttt tccgcgtctc tttttactga gctgaagcag
     9721 atgatgctgg cggtaccatc cccgtaacac tgacagaaca gatttctccg taccatcgat
     9781 gcatgcagag cactaaccag aaaaatcctc gctgtgtggt cctgagcggt caaccgagag
     9841 tcaatgcctg ctacagcatt tatgaaaatc gctcatccac ctgcagggaa tttcctatgt
     9901 ccggcaacaa tggtgtggtt aatgaagcat gtaaccgtgc gcgagcgaaa tacgacctaa
     9961 ccccacttta ctaaacgcga tcggtatcgc tgtcatgaca tattttgtat gccattattc
    10021 tgacgactta acaactcact tattgtaatc aagtttcttt cttctggtcg gggagctgtg
    10081 cagctcccct tttttattgc atcattaagg cagccactgc acagagcgtt accaccgtac
    10141 ccagccacac aaaaagcatg aaacacatcg ctcctgctgt gtagcgcttc tttacgtatt
    10201 tcagatttgc ctgtttcatg acaatttacc agattattct tcacccccgg ataacaaaac
    10261 gcggctatgc ccgccccaca agatgcttca cacttctgta cctcaggccg tttttaccaa
    10321 gccccatata cagaaaggtg ttcactgtct tcgtgtgaat tcccatttca cgggcaagat
    10381 gacacggctt tgactcccgg tatacataac caaacatcac caccatctct gacatcgtca
    10441 gcggacgtgc gtatgtgttc tgttcccagt caccgttacg gattttcctg tccgtttcca
    10501 gacgccctgt aatctcctcc gcatccagtt gaagataacg gcgtccctga aacatacagc
    10561 gcatcagacg actcaccata gcatcacaga taatcaccca ggactcatca tgggtcggta
    10621 cactcttcct gttctccggg ataaaaaatt ccctcgccag tatcaccctg taaccttcca
    10681 gcatctgttg cagaccgtac tccagataac ggttacgggt cgccagaatc actttcatcg
    10741 cagccccctg tttcatgctc acatgagaaa aaaacggcag gccaaatccc ctgcctgccg
    10801 tcatgcttca tgtcactgtc cgcaattaaa actcgaagtt cacaccaacg ttgtaggata
    10861 catcaccgtc gctggctgca atacctgctt tggctgctgt ctgctcgtta aagcggtaac
    10921 ccacaccaac ggccatagcc tgtttgtcac tgtaaccacc cagtgctgcc gtggcgttga
    10981 atttacccac actgtacggc tggaacaaac ctgccagtgc cgcactctgt gcagcagcac
    11041 gtttcatttc ttcgtggttt tcacgaatct gacgctgctg actgttcagc cgcgcgttat
    11101 gctcctgcag ctggcgggag tgattctgga tagtctgagt gtttttggtg acacgggtgt
    11161 caatgccacc aaccgcagca tcaatacggc cagttcttcc cgacagggaa acaaggcctt
    11221 cacttaccgc aacagttttt gtggctcctt cagcagtggt caggctgccg tctgaattca
    11281 cgccaacagt ggttctgtct ttcagatttt tatccagatc aacaattgca gcgttcacac
    11341 gaacagtatc aggcgcggtc tgatccaggg tatagctgcc atctttgttc ttacgaattg
    11401 ttgtatcggc ttttacctct gcaaccacat ccttaacagg aatgctttcc actccttttt
    11461 cataggtaat ttcaccattt tcatcaagag caaatgtagt ggcatttttc aggtcattta
    11521 catttgtgcc gagacggttg aattcctgta acagcgctcc attaccatct ttagccgctg
    11581 ttagcgcagc ttctgaactc atgccgttac tgatagcaat attgtaatct tttgcagttt
    11641 tactggtgat aactgaatta acaccatcaa tattagattt tattttttca atatcagtca
    11701 cttttagatt gaaaatattt tcagtcgtta acgaagggag cccactgggt aaagagactg
    11761 tataacgccc cgaattacca gcagcctgag ccaccatctg tttaatgttt ggatcatctt
    11821 tatttatttt tgggttagcg gcataaaaac tatctatagc agat
//
LOCUS       DAZGXZ010000149        11515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000149 DAZGXZ010000000
VERSION     DAZGXZ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.149"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            196..813
                     /gene="yijF"
                     /locus_tag="ACHKJF_004371"
     CDS             196..813
                     /gene="yijF"
                     /locus_tag="ACHKJF_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP0951622.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(840..1745)
                     /gene="yijE"
                     /locus_tag="ACHKJF_004372"
     CDS             complement(840..1745)
                     /gene="yijE"
                     /locus_tag="ACHKJF_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP0951623.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(1839..4019)
                     /gene="katG"
                     /locus_tag="ACHKJF_004373"
     CDS             complement(1839..4019)
                     /gene="katG"
                     /locus_tag="ACHKJF_004373"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP0951624.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYHKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKFTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4348..5238)
                     /gene="metF"
                     /locus_tag="ACHKJF_004374"
     CDS             complement(4348..5238)
                     /gene="metF"
                     /locus_tag="ACHKJF_004374"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP0951625.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5314..5448
                     /locus_tag="ACHKJF_004375"
     CDS             5314..5448
                     /locus_tag="ACHKJF_004375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951626.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5587..8019)
                     /gene="metL"
                     /locus_tag="ACHKJF_004376"
     CDS             complement(5587..8019)
                     /gene="metL"
                     /locus_tag="ACHKJF_004376"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP0951627.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(8022..9182)
                     /gene="metB"
                     /locus_tag="ACHKJF_004377"
     CDS             complement(8022..9182)
                     /gene="metB"
                     /locus_tag="ACHKJF_004377"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP0951628.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9459..9776
                     /gene="metJ"
                     /locus_tag="ACHKJF_004378"
     CDS             9459..9776
                     /gene="metJ"
                     /locus_tag="ACHKJF_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP0951629.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9960..10568
                     /gene="yiiX"
                     /locus_tag="ACHKJF_004379"
     CDS             9960..10568
                     /gene="yiiX"
                     /locus_tag="ACHKJF_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP0951630.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10713..11388
                     /locus_tag="ACHKJF_004380"
                     /pseudo
     CDS             10713..11388
                     /locus_tag="ACHKJF_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2808 a         3202 c         2897 g         2608 t
ORIGIN      
        1 tctctgcaat atattgaatt tgcttgtttt ttaggccaga taaggcgttc acgccgcatc
       61 cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt tatccgcttc
      121 tggacatttt ctctacagaa caatcgttat cccggaaggt cgaatctgtg actcttattt
      181 tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac ggcatttacc
      241 agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca ggccaatacc
      301 aaccttgcca tcgcagacgg tgccagacag caaatcggca gcacgctatt ttacgatccg
      361 gcgtatatgc agcttaccta tcctggcggt gatgtcccgc aagaacgcgg ggtatgttcc
      421 gatgtggtga tccgtgcatt gcgcagccag aaggtcgatt tgcagaaact ggtgcacgaa
      481 gatatggcga agaattttgc cgagtacccg caaaaatggc agttaaagcg cccggacagc
      541 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttacccgcca cgataaaaca
      601 cgccccatca gcaagaaccc cagtgactat caagcgggcg atattgtctc ctggcgatta
      661 gataacgggc tggcacatat tggggtggtt tcagatggct tcgcccgcga cggtacgccg
      721 ctggtgatac ataacattgg cgcaggtgcg caggaagaag atgtgctgtt tagctggcgg
      781 atggtggggc attatcgtta ttttgttaaa taagcgatcg ccccacatga agaaaaaatt
      841 cagatccttt ttacactaac ggcttctttt ttcttacggc tcaccagcgc cagtgccatc
      901 acaatcagca caataccgct accttcaacg gcccccggat tctcgccgag cagccaccag
      961 gaaaagagta cgccgcaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
     1021 aagtttttca atacaaacaa ccataagctc cacgccagtg ccgtcgccag aatcgcactg
     1081 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
     1141 gcgaccacac tcatcaccag cgccgcatag agcatctgcc aggatgttaa cgacaataaa
     1201 tccacgcgcg gatgacgggc atacagacgt ttagcaacaa tcgcgctcgc cccccaactg
     1261 acgccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
     1321 tgcaacacca aaaataagcc gaaagcggca atcagtatcg cgaaatattg cccacgtcgc
     1381 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctt
     1441 aggatcgcca ccttccccgc tccgccgctg accagcgccc actgcgccag gccaaccatc
     1501 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggtgtcgg gcgcattccg
     1561 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
     1621 gtaaagtcga aggcaccgat gtagctggtg acttgcttca tgaaaatcca gctataactc
     1681 cagataagtg tgagcacaac caggccactg attgccagtg ggttgctctt tcctgcggca
     1741 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
     1801 agcgactgcc tgaaaggcag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
     1861 aggttcatca ctttcaccca tgccgccacg aagtctttaa caaacttctc gtgagcatcg
     1921 ctgctggcgt aaacttcagc caccgcacgc aggacagagt tagaaccaaa taccagatcc
     1981 gcacggctgg ccgtaaattt cacttcgccg gtttcacggt cacggccttc gaacagctct
     2041 ttcgattcgt cggtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
     2101 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
     2161 cccagtacac gcatgccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
     2221 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
     2281 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
     2341 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
     2401 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
     2461 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
     2521 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
     2581 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
     2641 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
     2701 tgcggcagcg gatcttgcca gatcagatct tctttcggta cttccggtcc gatgtaacga
     2761 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
     2821 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
     2881 agcgtcaggt cggtcaccag catcgtcggt ttacgtttct tcgacggatc gaacggatcc
     2941 gggataattt ccggtgcgtc taccgcttcg aactggattg cgccagccgg actgcgggtc
     3001 tgtacccact catatttgaa caggttctcg aagaaatagt tgctccactg ggtcggcgtc
     3061 tgggtccaga ccacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
     3121 gtgctcgccc aacctaaacc ttgttcttca atcggtgcag cttctggatc aggacctaca
     3181 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
     3241 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
     3301 gaaagcggtt cgccgctgtg gtccgggcct tccgggttaa cgtaaatcag gcccatctcg
     3361 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
     3421 tcatcacccc agttaacgtc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
     3481 ccgaaggtac ggaagccgga gttttccagc gccacgttac ccgcgaggat aaacaggtcg
     3541 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
     3601 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
     3661 gcgccgccgc gcccgtcgat tgaacggtaa gtacccgcac cgtgccaggc catacgaata
     3721 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gttgagattc tgtcagcagg
     3781 gctttcagat cttttttcag accgtagtaa tctaatttgc tgaattcttt gtggtagtca
     3841 aagtcctcac ccagtgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
     3901 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
     3961 tgatggaacg ggcatttgcc agtggctgtg gtgttatgaa tatcgtctga cgtgctcatc
     4021 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
     4081 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
     4141 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
     4201 aaaaagggtt ttagctttat atccttcaag taatgaggga gaaaacaagg ctgacaacct
     4261 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
     4321 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
     4381 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
     4441 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
     4501 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
     4561 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
     4621 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
     4681 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
     4741 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
     4801 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
     4861 accacttccc ggcggcagat cgccacgcag cgccacaata tgacgaatac cgttattcca
     4921 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
     4981 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
     5041 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
     5101 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
     5161 cgaaacgtta atttgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
     5221 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
     5281 cacatctatc cgtttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
     5341 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
     5401 aagaaaaaga aaatccggta tgcaaagcaa accggactgc aaatataaac taaccatgta
     5461 cttattgctt cttgccggat tcgtcgtaaa cgcttaatcc ggcctaatcc tatgataatt
     5521 caatatattt cattagaaac cataaacccg aaaacatgag taccgggcat tattaaattt
     5581 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
     5641 gtcgcgcccc gcgccaggcc cacggatcac cagcgggttg tcgcgatacc agcggctttc
     5701 gatggcaaag acattatcgc acggcagcag tgatgccagc ggatggtctt cacgcaccgc
     5761 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
     5821 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
     5881 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
     5941 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaattacca gcttgcgcat
     6001 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
     6061 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
     6121 cgtgccggag aagatcccgc tgatcgacaa aatagtatcg ccgctgtcga tcagatcgcg
     6181 cacggtgtgg ttgatcggca agcccgcacc gacggtggca ttgtacagcc agtgacgccc
     6241 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgctgg ctcccgccag
     6301 tttgttggcg ctgataacgt ggaaaccgtg gctggcgaaa tcaagatact gatcagcaag
     6361 ctgctggctg gcggtaacgt ccagcaccac taaatcatca tacggatggg cgcgcatcca
     6421 caggaacaac gactcttcat cctgctcaac cgcttcatcg ttgaagaagg ctaacgcgcg
     6481 gctggcgtcc agcccgtcat agctcaacag gctgcggcgg ctgtccacca cacctgccag
     6541 cacaaactca aagccggtac gtgccgaaag cgtgctctgc tcacgggcga acagttccag
     6601 ccaacgggaa ccgatattgc ccttaccgaa caataccagg ccgatgcgtt tttctgcgcg
     6661 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
     6721 caccaggctg atgccgtcat cggactgcca ggtaaattcg accggctggc ctttcagttg
     6781 ctgccagaag cggtggcaat gcagcgggtt acgggtgacg cctgcaccga ccatcgccac
     6841 cagcgccagc ccctgacgca ggcgcagttc gccaggtaat cccgcttcgt cgaggatttt
     6901 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc agcaactggc gatcgttatg
     6961 tacgccaacc gccagcgggc gtacctgcgc gcgtttcagg atttggtcga tctctttatg
     7021 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
     7081 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
     7141 ttgatccggc gtgtagctac agcgcagttg caggtcgatt tcgctgccag aaaccggctg
     7201 taaagtacgg gcgtgaagaa cgggagccgc caggcgcgcc agttcgctgg cctcatccag
     7261 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
     7321 ggcgacgtcg ctccagatgg ttacgcgaga aacacccgcc agcgcaccga tttgtgtcgc
     7381 ggaatagtcg gaaccgttac gccccagcag caccgtttca ccggcgttgt tgcggctgat
     7441 aaatccggtc accaccagac gtttgcccgg atgttgcacc agcagctgtt gcagcaacgg
     7501 gtaagaaagc ccttcatcaa cctgcggttg tgcggcgcgt tcagcgcgta aaaactcgcg
     7561 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgtgc
     7621 cgaccacact tcaccgtgcc ccaccacttc cgcatacact gcgtcgttaa taccgctgtc
     7681 gagcagcgcc gccaggcgct caaggtcgct gacaaaagcg ctaatgaggc tatcggcttc
     7741 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
     7801 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
     7861 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagtact ccgccataat
     7921 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
     7981 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
     8041 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
     8101 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
     8161 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
     8221 aacaacgaca gcccgctcag gaaacgacgc agcgtctgct catcgccatc cagttcaaaa
     8281 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
     8341 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
     8401 gcctgcgcgt tacgctgggc caactccatg cgcggcacca gcgttcgcaa cccgcgtagc
     8461 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
     8521 tcagtgacaa cgtccgggtc tttagcaatc accacgccgg ccaccacgtc tgagtgaccg
     8581 ttcagatatt tcgtgcatga atgcaacacc agatcggcac ctaatgccag cggattttgt
     8641 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
     8701 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
     8761 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
     8821 aacaacacgc gatagcaacc acgtttcgcc aggctgtcga acaggcgata gctaccgccg
     8881 tagcagtcgt gcggcgctac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
     8941 tgaatcgcgg acatgccggt attagtaagt actgcaccag caccgccttc cagttctgcc
     9001 agtgctcgct gaaccacatc gcgcgttggg ttgccacgac gcgagtaatc atgcgcgcgc
     9061 ggttcattaa atccggtaaa gttataggta ctggaaagat ggatgggtgg gacaacgcaa
     9121 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
     9181 atgtgatgat gttccctggg ctttattggt gaaatgtcag gcaccagagt aaacattgtg
     9241 gtaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
     9301 caaatagccg ttaaaattat atgcattatc acgccagcag gtggattacc cgatgtcacg
     9361 gtagcgcctg tacggtaaac tatgcgggtt tacggccagt gcccacatta actgtgtggt
     9421 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
     9481 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
     9541 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
     9601 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
     9661 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
     9721 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
     9781 gaaaagagcg gcgcggagtg gaaccgcctg atgcgctacg cttatcaggc ctacgtcata
     9841 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
     9901 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
     9961 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    10021 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    10081 tggcaaccca ttccgattac agccacaccg gtatgctggt gatacgcaac aaaaagccct
    10141 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg atcgcccatg
    10201 gtgaaaaggg caaatacgtc gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    10261 aaaaactggc gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    10321 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    10381 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    10441 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    10501 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    10561 cgtggtaatg gatgatgaaa aaaataattc ccgccgtagt ctgtttgttg ctttgtagtt
    10621 gcacgacctt tccgcaagct gtcgctccgg taaacggtga tgcctccaat tagttgaatt
    10681 aaggatataa tactcgcttt taaggttgtt tcatggtcac agttaacctt cattgccccc
    10741 gttgccagtc cgttcaggtt taccgtcacg ggcagaaccc aaaaagacat gatcgattcc
    10801 gcggccgtga ctgtcaccgg gtatttcagc tcagttactg ttacgagccc cgtaaaccgg
    10861 gagttaaaga ccaaatcgct gaaatgactt tcaacggtgc cggggttcgc gatactgccc
    10921 gtacgctgaa aatcggcatt aatacagtca tccgaacttt aaaaaactct cgccacgaag
    10981 aataacgtca tccccggtcg ctcatgctga tgtggcactc atctgcaaac ttagtcagca
    11041 atggagcttt gtcggcagta aagcgcgaca gcactggctc tggtacgtgt acaacacgaa
    11101 aactggtggt gttctggcct acacttttgg cccccggact gacgaaacct gtcgtgaatt
    11161 gcgggcgctg cttacccttt taccatcggc atgttgacca ctgacgaatg gggtagttac
    11221 gctagagagt taccgaagga gaagcatctg actggtaaga tttttactca gcacattgag
    11281 cgtaacaatc tgaccctgag aacccgaatc aaacgtctgg cccgcagaac catctgtttc
    11341 tcgcgttctg tagaacttca tgaaaaagtc atcggtgctt ttatcgaaaa tatatgttct
    11401 actaattgga gtcatcaccc ttctgataag ctttccttgg atgattacca gactgaatac
    11461 acagccaaag atttttacct cattctgctc ttttttctca aatgacatat attat
//
LOCUS       DAZGXZ010000150        11506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000150 DAZGXZ010000000
VERSION     DAZGXZ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.150"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(51..1415)
                     /gene="rhlE"
                     /locus_tag="ACHKJF_004381"
     CDS             complement(51..1415)
                     /gene="rhlE"
                     /locus_tag="ACHKJF_004381"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP0951631.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            1644..2315
                     /gene="cecR"
                     /locus_tag="ACHKJF_004382"
     CDS             1644..2315
                     /gene="cecR"
                     /locus_tag="ACHKJF_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP0951632.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2318..3313
                     /gene="hlyD"
                     /locus_tag="ACHKJF_004383"
     CDS             2318..3313
                     /gene="hlyD"
                     /locus_tag="ACHKJF_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP0951633.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            3306..5042
                     /gene="ybhF"
                     /locus_tag="ACHKJF_004384"
     CDS             3306..5042
                     /gene="ybhF"
                     /locus_tag="ACHKJF_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0951634.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5035..6168
                     /gene="ybhS"
                     /locus_tag="ACHKJF_004385"
     CDS             5035..6168
                     /gene="ybhS"
                     /locus_tag="ACHKJF_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0951635.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6179..7285
                     /gene="ybhR"
                     /locus_tag="ACHKJF_004386"
     CDS             6179..7285
                     /gene="ybhR"
                     /locus_tag="ACHKJF_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0951636.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7247..7657)
                     /gene="ybhQ"
                     /locus_tag="ACHKJF_004387"
     CDS             complement(7247..7657)
                     /gene="ybhQ"
                     /locus_tag="ACHKJF_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP0951637.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7790..8551
                     /gene="ybhP"
                     /locus_tag="ACHKJF_004388"
     CDS             7790..8551
                     /gene="ybhP"
                     /locus_tag="ACHKJF_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0951638.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8548..9789
                     /gene="clsB"
                     /locus_tag="ACHKJF_004389"
     CDS             8548..9789
                     /gene="clsB"
                     /locus_tag="ACHKJF_004389"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP0951639.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9789..10745
                     /gene="ybhN"
                     /locus_tag="ACHKJF_004390"
     CDS             9789..10745
                     /gene="ybhN"
                     /locus_tag="ACHKJF_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP0951640.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10781..11494)
                     /gene="ybhM"
                     /locus_tag="ACHKJF_004391"
     CDS             complement(10781..11494)
                     /gene="ybhM"
                     /locus_tag="ACHKJF_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0951641.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
BASE COUNT         2654 a         3053 c         3116 g         2683 t
ORIGIN      
        1 ttttgcgttt gttcatcagc ctgatgccgg gcatagcccg gcataaaaga ttactgcgca
       61 gcggtaggtt tacgcggacg gcggcgacgt tgttgttcgc ctgccggttt ggcatcgccg
      121 aggcggcgag acggtttttc tgcaggtttc gcgcttgcag attttgcgcc accttcccca
      181 cggcgtggtt gctgttgacc gcgaccacca ccttgcccac gaccgccgcc gccacgttgc
      241 tggcgaccgt tctggatcgg ttcggctttg attgacgggt ccggctcata gcccggaatc
      301 gcaatgcgcg ggatctcttt tttcagcagt ttttcgatat cacgcagcag tttgtgttca
      361 tcaacacaca ccagcgacaa cgcttcaccg gtagcagccg cacgaccggt acgcccgata
      421 cggtggacat aatcttccgg tacgtttggc agttcatagt tgacgacgtg cggcaactct
      481 tcgatatcca gaccgcgcgc agcgatatcg gtcgccacca gtacgcgaat gtcacccgat
      541 ttaaaatcag ccagcgcacg ggtacgcgca ccttgcgatt tattcccgtg gatcgccgca
      601 ctacggatgc catctttatt gagctgctca gccagatggt tagcgccgtg tttggtacgg
      661 gtaaacacca gcacctgctg ccagttccct ttaccaatca tgtgcgacag caattcgcgt
      721 ttgcgtttct tatcaacaaa gtgaacgtgc tgggtcacct gatcagacgc ggtattgcgg
      781 cgtgccactt cgatttccag cgggttgtgc aacagttttt ccgccagggc tttaatatcg
      841 tcagagaagg tcgcagagaa cagcaggttc tggcgtttcg ctggcagttt tgttaacacg
      901 cgacggatat cgtggataaa gcccatgtcg agcatgcggt cagcttcatc gaggacgagg
      961 atttcaacct gatccagctt cactgcattt tgatgttcca ggtccagcaa acgtcccggt
     1021 gttgccacca gaacatcaac gccgccgcgc agcttcatca tttgcgggtt aatgctgaca
     1081 ccgccaaaca ccaccagcga acgaatgttc aaatatttgc tgtaatcacg gacgttttcg
     1141 ccaatctgcg cagccagttc acgggtcggg gtaagaatga gcgcacgtac cggacgacgc
     1201 cctttggcgt gcggctggcg agtgatcagg tgttgcaaca gcggcagcgt aaagcccgct
     1261 gttttaccgg taccggtctg ggcgctagcc atcaggtcgc ggccttccag caccgcaggg
     1321 atcgcctgct gctgaatagg ggtgggttca cggtaaccct gctcggcaac ggcgcgcagg
     1381 atatcagggc ttaaacccaa agaatcgaaa gacataacta ctccgaaccg ccccgaccgt
     1441 taccggtgta gttttcaggg agatactgaa aaggaaaatg acaaaaacca caatgtcatg
     1501 agggagcgga gtgtagcagt ttttgtgacg cagcgcataa attatccctg tgactggcgt
     1561 agctaaaatt cttagccata ctggacaact cccatatgga gtcataatct taatcaatca
     1621 tttgattaag aatggactgt gcgatgaata atcctgccat gacaatcaag ggtgaacagg
     1681 cgaaaaaaca gctgattgct gccgcactgg cgcagtttgg tgaatatgga atgaacgcca
     1741 ccacccgcga gatagccgcc caggccgggc agaatatcgc tgccatcacc tactacttcg
     1801 gttcgaaaga agatttgtac ctcgcctgcg cccagtggat tgccgatttt attggcgagc
     1861 agttccgtcc gcacgccgaa gaggccgaac gcttgttcgc acaaccacag cctgatcgcg
     1921 cagccatccg tgaactgatc cttcgcgcct gcaggaacat gattaagctg ctcacccagg
     1981 atgacaccgt caacctcagc aagtttatct cccgtgagca gctctctccc acggcagcct
     2041 atcacctggt gcacgaacag gtgattagcc cgctacacag ccacctgaca cgcctgattg
     2101 ccgcctggac cggctgcgac gccaacgata cccgcatgat cctccatact catgcgttga
     2161 ttggtgagat tctggcgttc cgtcttggca aagaaacaat tctgttacgt accggctgga
     2221 ccgcgttcga tgaagaaaag accgaactga tcaaccagac ggtgacctgt catatcgacc
     2281 tgattttgca aggattatcg caaaggagtt tgtagtgatg aaaaaacctg tcgtgatcgg
     2341 actggcggta gtggtacttg ccgccgtggt tgccggaggc tactggtggt atcaaagccg
     2401 ccaggataac ggcctgacgc tgtatggcaa cgtggatatt cgtacggtga atcttagttt
     2461 ccgtgttggg gggcgcgttg aatcgctggc ggtggacgaa ggtgatgcta tcaaagcggg
     2521 ccaggtgctg ggcgaactgg atcacaagcc gtatgagatt gccctgatgc aggcgaaagc
     2581 gggtgtttcg gtggcacagg cgcagtatga cctgatgctt gccgggtatc gcgatgaaga
     2641 aatcgctcag gccgccgcag cggtgaaaca ggcgcaagcc gcctatgact atgcgcagaa
     2701 cttctataac cgccagcaag ggttgtggaa aagccgcacc atttcggcaa acgacctgga
     2761 aaatgcccgc tcctcgcgcg accaggcgca ggcaacgctg aaatcagcac aggataaatt
     2821 gcgtcagtac cgttccggta accgtgaaca ggacatcgcc caggcgaaag ccagcctcga
     2881 acaggcgcag gcgcaactgg cgcaggcgga gttgaatttg caggactcaa cgttgatagc
     2941 cccgtctgat ggcacgctgt taacgcgcgc ggtggagcca ggcacggtcc tcaatgaagg
     3001 tggcacggtg tttaccgttt cactaacgcg tccggtgtgg gtacgtgcct atgttgacga
     3061 acgtaatctt gaccaggccc agccggggcg caaagtgctg ctttataccg atggtcgccc
     3121 ggacaagccc tatcacgggc agattggttt cgtttcgccg actgctgaat ttaccccgaa
     3181 aaccgtcgaa acaccggatc tgcgtaccga cctcgtctat cgcctgcgta ttgtggtgac
     3241 cgacgccgat gatgcgttac gccagggaat gccggtgacg gtgcaattcg gtaacgaggc
     3301 aggacatgaa tgatgccgtt atcacgctga acggcctgga aaaacgcttt ccgggcatgg
     3361 acaagcccgc cgtcgcgccg ctcgattgta ccattcacgc cggttatgtg acggggctgg
     3421 tggggccgga tggtgcaggt aaaaccacgc taatgcggat gctggcggga ttactgaaac
     3481 ccgacagcgg caatgccacg gtgattggct ttgatccaat caaaaacgac ggcgcgctgc
     3541 acgccgtgct cggctatatg ccgcagaaat ttggtctgta tgaagatctc acggtgatgg
     3601 agaacctcaa tctgtacgcg gatttgcgca gcgtcaccgg cgaagcacgt aagcaaactt
     3661 ttgctcgcct gctggagttt acgtctcttg ggccgtttac cggacgcctg gcgggcaagc
     3721 tctccggtgg gatgaaacaa aaactcggtc tggcctgtac cctggtgggc gaaccgaaag
     3781 tgttgctgct cgatgaaccc ggcgtcggcg ttgaccctat ctcacggcgc gaactgtggc
     3841 agatggtgca tgagctggca ggcgaaggga tgttaatcct ctggagtacc tcgtatctcg
     3901 acgaagccga gcagtgccgt gacgtgttac tgatgaacga aggcgagttg ctgtatcagg
     3961 gggaaccaaa agccctgaca caaaccatgg ccggacgcag ttttctgatg accagtccac
     4021 acgagggcaa ccgcaaacta ttgcaacgcg ccttgaaact gccacaggtc agcgacggca
     4081 tgattcaggg gaaatcggta cgtctgatcc tcaaaaaaga ggccacacca gacgatattc
     4141 gccatgccga cgggatgccg gaaatcaaca tcaacgaaac tacgccgcgt tttgaagatg
     4201 catttattga tttgctgggt ggtgccggaa cctcagaatc gccgctgggc gcaatattac
     4261 atacggtaga aggcacaccc ggcgagacgg tgatcgaagc gaaagaactg accaagaaat
     4321 ttggtgattt tgccgccacc gatcacgtca actttgccgt taaacgtggg gagatttttg
     4381 gtttgctggg gccaaacggc gcgggtaaat cgaccacctt taagatgatg tgcggtttgc
     4441 tggtgccgac ttccggccag gcgctggtgc tggggatgga tctgaaagag agttctggta
     4501 aagcgcgcca gcatctcggc tatatggcgc aaaagttttc gctctacggt aacctgacgg
     4561 tcgaacagaa tttacgcttt ttctctggtg tgtatggctt acgcggtcgg gcgcagaacg
     4621 aaaaaatctc ccgtatgagc gaggcgttcg gcctgaaaag tatcgcctcc cacgccaccg
     4681 atgaactgcc attaggtttt aaacagcggc tggcactggc ctgctcgctg atgcatgaac
     4741 cggacattct gtttctcgac gaaccgacgt ccggcgttga tccgattact cgccgcgaat
     4801 tctggctaca tatcaacagc atggtagaga aaggcgtcac ggtgatggtc accacccact
     4861 ttatggatga agcagaatat tgcgaccgca tcggcctggt gtaccgtggg aaattaattg
     4921 ccagcggcac gccggatgat ttgaaagcgc agtcggccaa cgacgagcaa cccgatccca
     4981 ccatggagca agcctttatt cagttgatcc acgactggga taaggagcat agcaatgagt
     5041 aacccgatcc tttcctggcg tcgcgtgcgg gcgctgtgcg tcaaagagac gcggcagatc
     5101 gttcgcgatc cgagtagctg gctgattgcg gtagtgatcc cgctgttgct gctgtttatt
     5161 tttggttacg gcattaacct cgactccagc aagctgcggg tcgggatttt actggaacag
     5221 cgtagcgaag cggcgctgga tttcacccac accatgaccg gttcgcccta catcgacgcc
     5281 accatcagcg ataaccgtca ggaactgatc gccaaaatgc aggcggggaa aattcgcggt
     5341 ctggtggtta ttccggtgga ttttgcggaa cagatggagc gcgccaacgc caccgcaccg
     5401 attcaggtga tcaccgacgg cagtgagccg aataccgcta actttgtaca ggggtatgtc
     5461 gaagggatct ggcagatctg gcaaatgcag cgagcggagg acaacgggca gacttttgaa
     5521 ccgcttattg atgtacaaac ccgctactgg tttaacccgg cggcgattag ccagcacttc
     5581 attatccccg gtgcggtgac cattatcatg acggtcatcg gcgcgattct cacctcgctg
     5641 gtggtggcgc gagaatggga acgcggcacc atggaggctc tgctctctac ggagattacc
     5701 cgcacggaac tgctgctgtg taagctgatc ccttattact ttctcgggat gctggcgatg
     5761 ttgctgtgta tgctggtgtc agtgtttatt ctcggcgtgc cgtatcgcgg gtcgctgctg
     5821 attctgtttt ttatctccag cctgttttta ctcagtaccc tggggatggg gctgctgatt
     5881 tccacgatta cccgcaacca gttcaatgcc gctcaggtcg ccctgaacgc cgcttttctg
     5941 ccgtcgatta tgctttccgg ctttattttt cagatcgaca gtatgcccgc ggtgatccgc
     6001 gcggtgacgt acattattcc cgctcgttat ttcgtcagca ccctgcaaag cctgttcctc
     6061 gccgggaata ttccagtggt gctggtggta aacgtgctgt ttttgatcgc ttcggcggtg
     6121 atgtttatcg gcctgacgtg gctgaaaacc aaacgtcggc tggattaggg agaagagcat
     6181 gtttcatcgc ttatggacgt taatccgcaa agagttgcag tcgctgctgc gcgaaccgca
     6241 aacccgcgcg attctgattt tacctgtgct tattcaggtg attctgttcc cgttcgccgc
     6301 cacgctggaa gtgactaacg ccaccatcgc catctacgat gaagataacg gcgagcattc
     6361 ggtggagctg acccaacgtt ttgcccgcgc cagcgccttt actcatgtgc tgctgctgaa
     6421 aagcccacag gagatccgcc caaccatcga cacacaaaag gcgttactac tggtgcgttt
     6481 cccggctgac ttctcgcgca aactggatac cttccagacc gcgccgttgc agttgatcct
     6541 cgacgggcgt aactccaaca gtgcgcaaat tgccgccaac tacctgcaac agatcgtcaa
     6601 aaattatcag caggagctgc tggaaggaaa accgaaacct aacaacagcg agctggtggt
     6661 acgcaactgg tataacccga atcttgacta caaatggttt gtggtgccgt cgctgatcgc
     6721 catgatcacc actatcggcg taatgatcgt cacttcactt tccgtcgccc gcgaacgtga
     6781 acaaggtacg ctcgatcagc tactggtttc gccgctcacc acctggcaga tcttcatcgg
     6841 caaagccgta ccggcgttaa ttgtcgccac cttccaggcc accattgtgc tggcgattgg
     6901 tatctgggcg tatcaaatcc ccttcgccgg atcgctggcg ctgttctact ttacgatggt
     6961 gatttacggt ttatcgctgg tgggattcgg tctgttgatt tcatcactct gctcgacaca
     7021 acagcaggcg tttatcggcg tgtttgtttt tatgatgccc gccattctcc tttccggtta
     7081 cgtttctccg gtggaaaaca tgccagtatg gctgcaaaac ctgacgtgga ttaaccctat
     7141 tcgccacttt acggacatta ccaagcagat ttatttgaag gatgcgagtc tggatattgt
     7201 gtggaatagt ttgtggccgc tactggtgat aacggccacg acagggtcag cggcgtacgc
     7261 gatgtttaga cgtaaggtga tgtaacttct tatctttcgc cagcaaagac actaccgccg
     7321 ggccggcaag gattgccagc cctgcaattg ccagcaaaaa gttgttttcc agtacacggg
     7381 acaacagcca cagcacaatc agcgctgcgc caaaatacca ggtcgtggtc agctcttcca
     7441 gcacgtctgc cacctcacgc cagcgttgct cggggtggcg ctgaaacacc aacatcatca
     7501 ctaccgtgac acaatacagt gcgcataatc ccacgccgac gtgaaccaga gtcttactca
     7561 tccagcccac caccagcagc gccactacca gtaaatgcaa cataatctgc cagcaactta
     7621 gaccagttgc gacacgaaca cgttgttgcc acttcatggc ttctctcctg aagaatttat
     7681 ctctacttac tcagagtacc gaactttacg gaaaattccg taagaccgca cctttttgtg
     7741 acgccgacta cactatttca atgagataag aggcaaagac aggaaaatta tgcccgatca
     7801 aacacaacaa ttttcgttca aggtgctcac catcaacatt cacaaaggtt ttaccgcgtt
     7861 taaccgacgc ttcattttgc cggaacttcg cgacgcggtg cgtaccgtca gcgccgatat
     7921 tgtttgcctg caggaagtga tgggcgcgca cgaagttcat ccgctgcatg tggaaaactg
     7981 gcccgatacc tcgcactacg agtttctcgc cgacacgatg tggagcgatt ttgcctacgg
     8041 tcgcaatgcc gtatacccgg aagggcatca cggcaacgcc gtactgtcgc gttatcccat
     8101 tgaacattat gagaatcgcg atgtttcggt cgatggtgcg gaaaagcgcg gcgtgctcta
     8161 ctgccgcatt gtgccaccga tgaccggaaa agcgattcat gtgatgtgcg tacatctggg
     8221 cctgcgtgag gcgcaccgtc aggcgcagct tgcgatgctc gccgaatggg tgaatgagct
     8281 accggacggc gaaccggtat tggtggcggg tgatttcaat gactggcggc aaaaagctaa
     8341 tcatccgtta aaagtgcagg ccggactgga tgagattttt acccgcgccc acggacgccc
     8401 ggcgcgcacg tttccggtgc aatttcctct actacgactg gacaggatct acgtcaaaaa
     8461 tgccagcgcc agcgcgccaa ccgcgttgcc gctgcggaca tggcgacacc tttctgatca
     8521 tgccccttta agtgcggaga ttcatttatg aaatgtagct ggcgcgaagg caataagatc
     8581 cagttgctgg aaaacggcga gcaatattat cccgcggtgt ttaaggcgat tggcgaggca
     8641 caagaacgca tcattcttga aacgtttatc tggtttgagg atgacgtcgg caaacagctg
     8701 catgcggcac tactggcagc agcgcaacgc ggggttaaag cggaagtctt gctggatggc
     8761 tacggttcgc cggatctcag cgatgagttt gtcaatgaac tgacggcagc tggcgtggta
     8821 tttcgctact acgatccccg ccctcgcctt tttggtatgc gcaccaatgt gtttcgccgg
     8881 atgcatcgca aaattgtggt gatcgacgcg cgtatcgcct ttattggcgg gctgaattac
     8941 tccgccgagc atatgtccag ctacggtcca gaggctaaac aggattacgc ggtacgcctt
     9001 gaagggccga ttgtcgaaga tattctccag tttgagctgg aaaacctgcc tggacagagc
     9061 gcggcacgac gctggtggcg acgtcatcac aaagcggaag agaaccgcca gccgggagaa
     9121 gcgcaggtat tgctggtctg gcgcgataac gaagaacatc gcgatgatat tgaacgtcac
     9181 tatctgaaaa tgctcactca ggcgcggcga gaagtcatta tcgccaacgc ctacttcttc
     9241 cccggctatc gatttttaca cgccttgcgt aaagcggcac ggcgcggggt gcggatcaaa
     9301 ctgatcattc agggcgaacc ggatatgccg attgtcagag tcggtgcgcg cttgctgtat
     9361 aactatctgg ttaaaggcgg cgttcaggta tttgagtacc gccgccgccc gctccacggc
     9421 aaagtggcat tgatggacga tcactgggcg acagtagggt ccagtaatct cgatccgctc
     9481 agtttgtcac tgaatctcga agcaaatgtc atcatccacg atcgtcattt taaccagacg
     9541 ctgcgcgata atctgaacgg cattattgcc gcagattgtc agcaggtcga tgagaccatg
     9601 ctgccgaaac gcacctggtg gaacctgacc aaaagcgtgc tggcgttcca ctttttacgc
     9661 cacttcccgg cgctggttgg ctggcttccg gcacacacac cacgtctggc gcaggttgat
     9721 ccgcccgcac aaccgacaat ggaaacgcag gatcgggtag aaactgaaaa cacgggggta
     9781 aaaccctgat gagtaaatca cacccgcgct ggcgcttagc aaagaagatc ctcacctggc
     9841 tgttttttat cgcggtgatt gtgttgctgg tggtctacgc caaaaaagtg gactgggaag
     9901 aggtctggaa ggtcatccgc gactacaatc gcgttgcgct gcttagtgcg gtcgggctgg
     9961 tggtcgtcag ctatctgatt tacggctgct atgacctgct cgcccgcttt tactgcggtc
    10021 acaaactggc gaagcgccag gtgatgctgg tgtcgtttat ctgctacgcc ttcaacctga
    10081 cgctcagtac ctgggtcggc ggcattggta tgcgctatcg tttgtactcc cgactagggt
    10141 taccgggcag cactattacg cggattttct cgctcagtat taccaccaac tggctgggct
    10201 atattttact ggcagggatt atctttaccg caggcgtggt ggagttgccg gatcactggt
    10261 atgtcgatca aactacgctg cgtattctcg gcattggctt actgatgatt atcgcggttt
    10321 atttgtggtt ttgcgctttc gcgaagcacc gccatatgac catcaaaggg caaaagctgg
    10381 tgctgccttc atggaaattc gccctcgctc agatgctgat ttccagcgtt aactggatgg
    10441 taatgggggc gattatctgg ctgttacttg gtcaaagcgt gaactatttc tttgtactgg
    10501 gcgtgttact ggttagtagt attgctggcg tcatcgtgca tattccggcg gggatcggtg
    10561 tgctggaagc ggtgtttatc gcgctactgg ctggggagca tacatccaaa ggcacaatta
    10621 tcgccgccct actcgcttat cgcgtcctgt attactttat cccgctgctg ctggcgctga
    10681 tctgttacct gctactggaa agccaggcga agaagctacg ggcgaaaaat gaagcggcga
    10741 tgtgaagttt agttaagttc tttagtatct gcatttacga ctaatgaaaa aaacgcgtat
    10801 gcctttgcca gacaagcgtt atagctatcg cggcaagtat cccaaagaag ccgataaaat
    10861 agtaatagag tgttaatgca cctgtaacag cgcaacgaac cggactccac aactcttcac
    10921 tatggcattt ccccgccaat tcaaggagct tatttcgtcc atgcgagata atgcctgacc
    10981 ataacacaat cattaagcaa cttattaccc aaacgggtcg ttcactcatt aatgcggcat
    11041 tcatgatgat caccagggcc aaacctgtca acgtcatcat aatgataaaa cggtgagaac
    11101 caggatcaac attaaataat aaaccggcca gcatactgat taaaaacatc gcgcccgcag
    11161 tgctgaacac gacgataatg gcaggtacag gagctaaact tgcaacgaca atcgccacca
    11221 aagagagtaa aaacagatat attacgcctg cgatgacggc atttatcttg ttgatatcct
    11281 tacttaagcc atacatacaa actaataata accctatggc gacatacatt gttggtgaat
    11341 catatttggt tgccgtaatt tcgagatact tagctcccca ggccagtcct gcggttgcca
    11401 ccaaacctaa agcggtaatg agccatatac cgtttaatat cctggtctcg tgctgaaaat
    11461 ctaatttatt gctgttttgt gaatatgatt ccattctttt agttgt
//
LOCUS       DAZGXZ010000151        11426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000151 DAZGXZ010000000
VERSION     DAZGXZ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.151"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            336..917
                     /gene="mdaB"
                     /locus_tag="ACHKJF_004392"
     CDS             336..917
                     /gene="mdaB"
                     /locus_tag="ACHKJF_004392"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP0951642.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            948..1262
                     /locus_tag="ACHKJF_004393"
     CDS             948..1262
                     /locus_tag="ACHKJF_004393"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP0951643.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1310..3202)
                     /gene="parE"
                     /locus_tag="ACHKJF_004394"
     CDS             complement(1310..3202)
                     /gene="parE"
                     /locus_tag="ACHKJF_004394"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP0951644.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3231..3812)
                     /gene="yqiA"
                     /locus_tag="ACHKJF_004395"
     CDS             complement(3231..3812)
                     /gene="yqiA"
                     /locus_tag="ACHKJF_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP0951645.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3812..4639)
                     /gene="cpdA"
                     /locus_tag="ACHKJF_004396"
     CDS             complement(3812..4639)
                     /gene="cpdA"
                     /locus_tag="ACHKJF_004396"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP0951646.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4664..5086)
                     /locus_tag="ACHKJF_004397"
     CDS             complement(4664..5086)
                     /locus_tag="ACHKJF_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP0951647.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5087..5716)
                     /gene="nudF"
                     /locus_tag="ACHKJF_004398"
     CDS             complement(5087..5716)
                     /gene="nudF"
                     /locus_tag="ACHKJF_004398"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP0951648.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5921..7402
                     /gene="tolC"
                     /locus_tag="ACHKJF_004399"
     CDS             5921..7402
                     /gene="tolC"
                     /locus_tag="ACHKJF_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP0951649.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7550..8221
                     /locus_tag="ACHKJF_004400"
     CDS             7550..8221
                     /locus_tag="ACHKJF_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP0951650.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8227..9387
                     /locus_tag="ACHKJF_004401"
     CDS             8227..9387
                     /locus_tag="ACHKJF_004401"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0951651.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9425..10213)
                     /gene="ygiD"
                     /locus_tag="ACHKJF_004402"
     CDS             complement(9425..10213)
                     /gene="ygiD"
                     /locus_tag="ACHKJF_004402"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP0951652.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10356..11129
                     /gene="zupT"
                     /locus_tag="ACHKJF_004403"
     CDS             10356..11129
                     /gene="zupT"
                     /locus_tag="ACHKJF_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP0951653.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11187..11357)
                     /gene="yqiD"
                     /locus_tag="ACHKJF_004404"
     CDS             complement(11187..11357)
                     /gene="yqiD"
                     /locus_tag="ACHKJF_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP0951654.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         2842 a         3067 c         2814 g         2703 t
ORIGIN      
        1 attttatgct ttaacatata tcagtacgct cataaaaatc catttataat tatcaacatg
       61 acacgttgat attttcatct tttacttatt caaagccata ttttcaatcc caaaaaaaac
      121 cgaaccaaac gtgtaaatta ttccggtgcg agtaagaatt atgaggaatg gctatcagta
      181 ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc agagcaagat cagacttttg
      241 ctaaattcgc aaaagacttt gcacattttg ctaatttcac cgtaccgctc tgtgacgtac
      301 tatagtcggc aaacgtctca ccttgaggtt aaaaaatgag caacatcctg attatcaacg
      361 gcgcgaaaaa attcgcccac tccaatggtc aactgaacga caccctgacc gaagtcgctg
      421 atggcacact gcgcgacctc gggcatgatg tccgcatcgt tcgcgccgac agcgactacg
      481 atgtcaaagc ggaagtacaa aactttctct gggctgatgt ggtgatctgg cagatgccag
      541 gctggtggat gggcgcgccg tggacagtga aaaaatatat tgatgatgta ttcaccgaag
      601 gtcatggaac gctgtatgcc agcgatggcc gtacccgcaa agatccgtca aaaaaatacg
      661 gttccggcgg cctggtacag ggcaaaaaat atatgctttc tctgacctgg aacgcaccaa
      721 tggaagcctt caccgaaaaa gatcaattct tccacggcgt tggcgtcgac ggtgtgtatc
      781 tgccgttcca taaagcaaac caattccttg gcatggaacc gctgccgaca tttatcgcta
      841 atgacgtgat aaaaatgcct gatgttcccc gctatactga agaatatcgc aagcatcttg
      901 tggaaatttt tggttaacta gagctcaggc tttagaagga gttaaccatg cttaccgtaa
      961 tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca ggcggtattg gatcagtttg
     1021 ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca cggctatgcg ccaatggtgg
     1081 attgcgctgc tggcgtgagt ttccagtcta tggcaccgga ttctatcgtg atgattgagc
     1141 agtgggaaag catcgcgcat cttgaagcgc atctgcaaac cccgcacatg aaggcgtaca
     1201 gcgaagccgt aaaaggcgac gtgctggaga tgaatatccg catcctgcaa cctgggattt
     1261 aaacctgcct tgtttgcccg gccatcctga ccgggcaatg ttctttcctt taaacctcaa
     1321 tctccgccat gtcgcctttc tcttgcaacc agttgcggcg atcttccgag cgtttcttcg
     1381 ccagcagcat atccatcatc gcgtcagtac gctgatcgtc ttcatcatcg atagtcaact
     1441 gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg caattgcatc gggttcattt
     1501 cccccagacc tttaaaacgc tggacgttcg gcttgccttt cttgcgtttt aattgctcaa
     1561 gtacgccctc tttctcttct tccgtcagcg cgtaataaac ctctttcccg agatcaatac
     1621 ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt caccaacgcg cggaagtgtt
     1681 ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc atcagagtcc gcatccgcga
     1741 ggatacagat tttgccataa cgaagctggc tcagatcgtc gctgtcagga tcgataccga
     1801 tcgctaccga aatatcatgc acttcctgcg aggccagcac ttcgtcggaa gaaacttccc
     1861 aggtgttaag gatcttacct ttcagtggca tgatcgcctg atattcgcga tcgcgcgcct
     1921 gcttggcaga tccgcctgcg gagtcacctt ccacaaggaa cagttcggta cggttaagat
     1981 cctgcgcggt acagtcagcc agtttgccag gcagtgctgg gccgctggtc agctttttgc
     2041 gcaccacttt tttagccgca cgcatacggc gctgggcgct ggaaatcgcc atctccgcca
     2101 gcagctccgc cgcctgaacg ttctggttca gccacaggat aaaggcatct ttcaccacgc
     2161 ctgaaacgaa tgccgcgcat tgacgcgaag agagacgctc tttggtctgc ccggcaaact
     2221 gcggatcctg cattttcacc gacagaacat aggcgcagcg atcccagata tcttccgccg
     2281 acagcttcac gccgcgcggc agaatgttgc ggtattcaca gaactcacgc atcgcgtcca
     2341 acaggccctg acgcagacca ttaacatggg taccgccctg catcgttggg ataaggttga
     2401 cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca cagtagcgcc cagtccacag
     2461 cttcagtatc gccagcgaaa ttaccgataa acggtttttc tggcagcgtc ggtaaaccgt
     2521 ttaccgcttc cgccaggtaa tcattcagac cgtcctgata gcaccagcgc tgttcggtgt
     2581 tgttgatctc atctttaaag gtaatctcaa cgccggggca cagtactgct ttggccttca
     2641 gcacatgcgt caggcgtgaa acagaaaaac gcgggctgtc aaagaaggtt tcatccggcc
     2701 agaagtgcac gctggtaccg gtattgcgtt taccgcaagt gccgacaacc tgtaaatcct
     2761 gcaccttttc gccattttca aaggcgatgt tataaacctg accatcgcgg cgcacgttaa
     2821 cttctacgcg cttcgacagg gcgttaacca ccgaaatccc cacgccatgc aggccgccag
     2881 agaactggta atttttgtta gagaatttac cgcctgcatg cagacggcaa agaatcagtt
     2941 caaccgccgg tacaccctct tccgggtgaa tatccaccgg catcccgcgc ccatcgtcaa
     3001 taacttctaa cgactggtca gcatgtaaga ttacatccac gcgttttgcg tgacccgcca
     3061 gtgcttcatc gacactgtta tcaataactt cttgccccaa atggttaggg cgagtggtat
     3121 cggtatacat ccccggacgg cggcgaaccg gctcaagccc ggtgagtacc tcaatggcat
     3181 cagcgttata agtttgcgtc atggtttaag ttagcaattc gagttgatcg tcagagatgg
     3241 tgcagaccaa gaaaatcgac gatcgggttg aaataatctt cgaagcccgt gaatgcgtgg
     3301 ttgccgcctt ctatgacagt ctggcggcag gaagcgtagt acgccaccgc ctggcggtaa
     3361 tccagcactt catctcccgt ctgttgcagc agccagatca aatccggcgc ttccagcggg
     3421 tcaatctgca tgactttgag atcgtaaata tggcgtgact ctagcacata ttgctgcccg
     3481 gtgtaggggt tctcgttctg accgagatag tccgtcagca gttcaaacgg gcgcaccgcc
     3541 gggtttacca ccactgcggg cagcataaaa cattgtgaca accaggtggc gtaatatccc
     3601 cccagtgacg aaccgacaat acccagcgaa tcgccgccat gttccaggac aatggattcc
     3661 agcagctctg ccgcgtcgga aggatacggc ggcaactgcg gaatgatcat ctcaacgtca
     3721 gggtgatgtt ccgccagcca gttttttaac aagctcgctt ttgcagagcg cggcgagctg
     3781 ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc ttctgaagcg gtatcaggtt
     3841 ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag cgtgccatca gcatgtaact
     3901 cgagagtacg ccagccgggc gcgatagtat ccagcgtaaa gttggaacag tgcggcttaa
     3961 actgcacaca ggtcgacggc gttgccagca ggcggcgacc gttccagtcg agatccagct
     4021 cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg aaacttcgcc agcacggtat
     4081 ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca actacaaccc gcaggtagcg
     4141 gatgatgatg cagcagcagc aacgtatggc gttctggcgc atcggccagt ttacgttcca
     4201 gccactcaag ctgaaactcg ctcagctcac cgtgcggcac gccaaacacc tggctatcca
     4261 gcaacaggat ttgccattgc tcaccaataa acacgcgctt cgccggggag atacccgcat
     4321 cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt gcccggcagc cagacgcagg
     4381 gcgcacgaaa acttgcgatg ccttcagcga aatgctgata ggccgcagag gattgatcct
     4441 gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg ctgatgtgga cgaatcgcct
     4501 ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa cagggcttcg tgcttttgtg
     4561 caaacaggtg agtatcggta atttgtaaaa tcctgactct ggcctcacca gccagaggaa
     4621 gggttaacag gctttccaaa tggtgtcctt aggtttcacg acgctagtaa accggaatcg
     4681 ccatcgctcc atgtgctaaa cagtatcgca accagtccgc taaaaactgg ttaatttgat
     4741 gcttttcgtc gcgttgatgc aactttttat taggataatc ataccgcgct ttgaagcgaa
     4801 aaatctgctg gcttgaacac acttcagcca ccatcgcgtc atgatacaga cgcaccgtca
     4861 ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg ttctattgtc accagggtag
     4921 tgtatcgggt cgattccaca atcgtcagcc gatattgtgc gtttgccacc tgatagctta
     4981 cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg caattgtgaa aagttcatct
     5041 cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg cttcatttat gcccactcat
     5101 tttttaacgc ttgatgatgc agctgcagcc attgcaaagc gatgaccgac gctgcgttgt
     5161 cgattttccc ctcttctacc cactggtatg cctgttcccg gcttaccaca tgaacgcgaa
     5221 tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc ggtcgtggcg tccacttcgc
     5281 ccaccataat tgacgaacgc tcactggtgc cccccgggct tgccaggaaa cttaacaccg
     5341 gtttggtccg tttgactatc agtcccgcct cttcaatcgc ttcgcgacgg gcaacatctt
     5401 ccacactttc accctcttct atcatcccgg caaccatctc cagtagccag ggggtttcac
     5461 tggtgtcgta tgcggcaatc cgaatctgct caatcagcac aacttcatca cgcactgggt
     5521 caaagggtag caagactgcg gcgtgaccgc gctcaaaaat ttccctccgt acctcatgac
     5581 tcatttgccc gttgaataaa cgatgacgaa atctataaag atctaatgaa aaaaagccgc
     5641 gataaagtgt ttctcgtgca ataatttcta catcgttttt gccaaatgta acgggcaggt
     5701 tgtctggctt aagcattgtt aatgtcctgg cactaatagt gaattaaatg tgaatttcag
     5761 cgacgtttga ctgccgtttg agcagtcatg tgttaaattg aggcacatta acgccctatg
     5821 gcacgtaacg ccaacctttt gcggtagcgg cttctgctag aatccgcaat aattttacag
     5881 tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa atgaagaaat tgctccccat
     5941 tcttatcggc ctgagccttt ctgggttcag ttcgttgagc caggccgaga acctgatgca
     6001 agtttatcag caagcacgcc ttagtaaccc ggaattgcgt aagtctgccg ccgatcgtga
     6061 tgctgccttt gaaaaaatta atgaagcgcg cagtccatta ctgccacagc taggtttagg
     6121 tgcagattac acctatagca acggctaccg cgacgcgaac ggcatcaact ctaacgcgac
     6181 cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg tcgaaatggc gtgcgttaac
     6241 gctgcaggaa aaagcagcag ggattcagga cgtcacatat cagaccgatc agcaaacctt
     6301 gatcctcaac accgcgaccg cttatttcaa cgtgttgaat gctattgacg ttctttccta
     6361 tacacaggca caaaaagaag cgatctaccg tcaattagat caaaccaccc aacgttttaa
     6421 cgtgggcctg gtagcgataa ccgacgtgca gaacgcccgc gcgcagtacg ataccgtgct
     6481 ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg gtagagcagc tgcgccagat
     6541 caccggtaac tactatccag aactggcagc gctgaatgtc gaaaacttta aaaccgacaa
     6601 accacagccg gttaacgcgc tgctgaaaga agccgaaaaa cgcaacctgt cgctgttaca
     6661 ggcacgcttg agccaggacc tggcgcgcga gcaaattcgc caggcgcagg atggtcactt
     6721 accgacgctg gatttaacgg cttctaccgg gatttctgac acctcttata gcggttcgaa
     6781 aactcgtggt gccgctggta cccagtatga cgacagcaat atgggccaga acaaagtggg
     6841 cctgagcttc tcgctgccga tttatcaggg cggaatggtt aactcgcagg tgaaacaggc
     6901 ccagtacaac tttgttggtg ccagcgagca actggaaagc gcgcatcgta gcgtcgtgca
     6961 aaccgtacgt tcctccttca acaacattaa tgcatctatc agtagcatta acgcctacaa
     7021 acaagccgta gtttccgctc aaagctcatt agacgcgatg gaagcgggct actcggtcgg
     7081 tacgcgtacc attgttgatg tgttggatgc aaccaccacg ctgtacaacg ctaagcaaga
     7141 gctggcaaat gcgcgttata actacctgat taatcagctg aatattaagt cagccctggg
     7201 tacgttgaac gagcaggatc tgctggcact gaacaatgcg ctgagcaaac cggtttccac
     7261 taatccggaa aacgttgccc cgcaaacgcc ggaacagaat gctattgctg atggttatgc
     7321 gcctgatagc ccggcacccg tcgttcagca aacatccgca cgcactacca ccagtaacgg
     7381 tcataaccct ttccgtaact gatgacgacg acggggcttc ggccccgtct gaacgtaagg
     7441 caacgtaaag atacgggtta tctgccgcat tcttccccct tctcgcttca atttcgacca
     7501 gccatcctct attctgatgg gtatttacca ctggtcccgg aagacaaaaa tgaaacggac
     7561 aaaatccata cgccacgcat cgttccgcaa aaactggagc gcgcgccatc tgacaccagt
     7621 cgctctcgcg gttgccactg tttttatgct ggctggctgt gaaaagagtg atgaaacagt
     7681 gtctctctat caaaatgctg acgactgttc agctgcaaac ccaggcaaaa gcgccgaatg
     7741 taccaccgcg tacaacaatg cgctgaaaga agccgaacgt actgcgccga aatacgccac
     7801 ccgtgaagac tgtgttgctg aatttggtga aggtcagtgc cagcaggcac cagcccaggc
     7861 tggcatggca ccagaaaacc aggcgcaggc ccagcaatcc agcgggagtt tctggatgcc
     7921 gctgatggcc ggttacatga tggggcgtct gatgggcggc ggcgcgggat ttgcacagca
     7981 gccgctgttc tcctcgaaaa acccagccag cccggcttac ggtaaatata ccgacgcgac
     8041 gggtaaaaac tatggcgcag cccagccagg ccgcaccatg accgtaccga agacagccat
     8101 ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc ggttttggtg aatctgttgc
     8161 caaacaaagc actatgcagc gtagcgcaac tggcacctct tctcgttcaa tgggtggctg
     8221 ataccgatgg aaagagtcag tattaccgag cgcccggact ggcgtgagaa agcccacgaa
     8281 tacggtttca attttcacac catgtacggc gagccgtact ggtgtgaaga tgcttactac
     8341 aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca ccgccgaact gcaccagatg
     8401 tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc tgatgaccaa attccgcatt
     8461 ccaaaacaca cctggagttt tgtgcgccag tcatggctga cgcaccagcc atcgctttat
     8521 tcgcgtcttg atctggcgtg ggatggcact ggcgaaccta aacttctgga aaataacgcc
     8581 gatacgccaa cgtcactata cgaggcggcg ttctttcagt ggatctggct ggaagatcag
     8641 cttaacgccg gtaacttgcc ggagggcagc gaccagttta acagtctgca agaaaaactg
     8701 atcgatcgct tcgttgagct gcgtgaacag tatggcttcc agttgctgca tctcacctgc
     8761 tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt tgcaggactg cgcaacggaa
     8821 gctgaaattg ctactgagtt cctctacatc gatgatatcg ggttaggtga aaaaggtcag
     8881 ttcacggatt tacaggatca ggtaatttcc aacctgttca agctgtatcc gtgggaattt
     8941 atgttgcgtg agatgttttc caccaagctg gaggatgcag gcgtacgctg gctggaaccg
     9001 gcgtggaaga gcattatctc caacaaggca cttctaccgc tactgtggga gatgttcccg
     9061 aatcacccga acctgctgcc cgcttatttt gcggaagatg atcatccgca aatggaaaaa
     9121 tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg tgtcgatcat tgaaaacggc
     9181 aaaaccattg aagcagcgga aggtccgtat ggcgaagaag ggatgattgt tcagcaattc
     9241 cacccgttac cgaaattcgg cgacagctat atgctgattg gtagctggct ggtgaacgat
     9301 caacccgccg gaattggcat tcgtgaagac cgtgcattga tcacccagga tatgtctcgg
     9361 ttttatccac atatttttgt tgagtaatca atgatgccgg atggcattcg ccatccggta
     9421 attgttaacc tatctgcacc gacagcatac tcaggctgcc catttctata ccatcgaccg
     9481 gaatggtaat tggctcctgc ccatcccacg cacctaagac atacaacaac ggcagatagt
     9541 gctccggcgt tgggttcgat aacgcgccac cttcatggtc gaggtaattc accagaggat
     9601 gctgctctac cgccccttgc cacgtcagat tcgctttcac atactcatta aacgacatcg
     9661 cccaaggata tggtgaacta tcaccgtgcc acttcactgt acgcaggtta tgcaccacgt
     9721 tgccactggc gaccaacatt attccttcat ctcgcagcgc tgccagtttg cgccccattt
     9781 cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc gggatatcgg
     9841 cgtcaggata catcttaatc agcacgcccc aggaaccgtg gtcaaagccc caggcttctt
     9901 tatccagcgt caccgggacc ggtgctaaca gctcaaccag atgctgtgcc agcacaggcg
     9961 aacccggagc aggataatgc gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa
    10021 tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg ggtaaaccag tgagccgaaa
    10081 ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa cttctgccag ctgcgggtat
    10141 acaaattatc ttccagcacg ttcatcggac taccgtgacc taaaaacaat gctggcatac
    10201 gtgttgaaga catgatgata tccttaacta aaggtgtcat tttgatatcc tcacaatacg
    10261 cttgttcggc ggagtaagaa cccggataac aatgatgatg atcatcagtt attttgacga
    10321 tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc agtacctctc attctgacca
    10381 tactggcggg ggcagccacg tttattggcg cgtttctcgg cgttctcggg caaaaaccct
    10441 cgaaccgctt actggcgttt tcgctaggat ttgcggcggg gatcatgttg ctcatctcat
    10501 taatggaaat gcttcctgcc gcactagcag ctgaaggaat gtcgcctgtg ttgggttatg
    10561 gaatgtttat cttcggtctg cttggctatt ttggcctgga ccgcatgttg ccacatgcgc
    10621 atccgcagga tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc aagcgcacag
    10681 ccattctgct cactctcggc atcagtctgc ataacttccc ggaagggatt gccacctttg
    10741 tcacggcgag cagcaacctg gagctgggat ttggcatcgc actggccgtc gcgttgcaca
    10801 atattcctga aggtctggcg gttgcaggcc cggtttatgc agcaacgggt tctaaacgca
    10861 cggcaattct gtgggcgggg atttctggat tggcagaaat tcttggtggt gtgctggcgt
    10921 ggttaatcct cggtagcatg atttcccccg tggtcatggc ggcaatcatg gcggcggttg
    10981 caggaattat ggtggcgctc tcggttgatg aattaatgcc gctcgccaaa gagattgacc
    11041 ctaataataa ccccagctat ggcgtactat gtggaatgtc agtgatggga ttcagtttag
    11101 tgctgctaca aacggcggga attggttaaa agcaacatca gaagttggcc agcgcatgga
    11161 caacttctgc tagcttcact aaagccttat tctacctttt cggctgtctc ttctctcgta
    11221 ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc tgtcctggcg aaacgcccga
    11281 caataacgtt tcaaatgcag gaaataaccc accacaacaa gagcaatcaa tacaatccag
    11341 taccaggcga taaacatcct tttaccctta atatttaaca atactaaaca tttagcgcat
    11401 aaatttcaca tatccttttt cggata
//
LOCUS       DAZGXZ010000152        11420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000152 DAZGXZ010000000
VERSION     DAZGXZ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.152"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..477
                     /locus_tag="ACHKJF_004405"
     CDS             <1..477
                     /locus_tag="ACHKJF_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP0951655.1"
                     /translation="AACQGLAGLCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTE
                     PLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLS
                     ARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            492..1349
                     /gene="menB"
                     /locus_tag="ACHKJF_004406"
     CDS             492..1349
                     /gene="menB"
                     /locus_tag="ACHKJF_004406"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP0951656.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1349..2311
                     /gene="menC"
                     /locus_tag="ACHKJF_004407"
     CDS             1349..2311
                     /gene="menC"
                     /locus_tag="ACHKJF_004407"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP0951657.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2308..3663
                     /gene="menE"
                     /locus_tag="ACHKJF_004408"
     CDS             2308..3663
                     /gene="menE"
                     /locus_tag="ACHKJF_004408"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP0951658.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3773..4039
                     /gene="pmrD"
                     /locus_tag="ACHKJF_004409"
     CDS             3773..4039
                     /gene="pmrD"
                     /locus_tag="ACHKJF_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP0951659.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(4033..4419)
                     /gene="arnF"
                     /locus_tag="ACHKJF_004410"
     CDS             complement(4033..4419)
                     /gene="arnF"
                     /locus_tag="ACHKJF_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP0951660.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4419..4754)
                     /gene="arnE"
                     /locus_tag="ACHKJF_004411"
     CDS             complement(4419..4754)
                     /gene="arnE"
                     /locus_tag="ACHKJF_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP0951661.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4751..6403)
                     /gene="arnT"
                     /locus_tag="ACHKJF_004412"
     CDS             complement(4751..6403)
                     /gene="arnT"
                     /locus_tag="ACHKJF_004412"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP0951662.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6403..7293)
                     /gene="arnD"
                     /locus_tag="ACHKJF_004413"
     CDS             complement(6403..7293)
                     /gene="arnD"
                     /locus_tag="ACHKJF_004413"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP0951663.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7290..9272)
                     /gene="arnA"
                     /locus_tag="ACHKJF_004414"
     CDS             complement(7290..9272)
                     /gene="arnA"
                     /locus_tag="ACHKJF_004414"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP0951664.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYATDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9272..10240)
                     /gene="arnC"
                     /locus_tag="ACHKJF_004415"
     CDS             complement(9272..10240)
                     /gene="arnC"
                     /locus_tag="ACHKJF_004415"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP0951665.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10244..11383)
                     /gene="arnB"
                     /locus_tag="ACHKJF_004416"
     CDS             complement(10244..11383)
                     /gene="arnB"
                     /locus_tag="ACHKJF_004416"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP0951666.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPVDIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2774 a         2969 c         3070 g         2607 t
ORIGIN      
        1 gcggcttgcc aggggctggc ggggctttgt ggggttattg tcgaaggcgg gcatccgggg
       61 ctgcaaaatg ctgaacaacg tgcggaacgt cagcgttccg atcgccaatg ggcgcagcgt
      121 tttcgcacag aaccgttaac ggcggtattt gccgactggt atcaacagcc tgtttttgcc
      181 tcgctcaatg acgatcaacg ccgggagctg gtggcgctgc gcagcaacaa taatggcgca
      241 acccttgccg ccatgctgga ggcgacttct ctcgccgtcc agcctgattt acgtgctaac
      301 cttagcgccc gcacatttgc gttttattat ttatgtggtg aacgtgacag caaattccgc
      361 gccctggcgg cggaactggc tgccgactgc catgtcattc ctcgcgccgg acataacgcg
      421 catcgggaaa atcccgctgg cgttatcgca agtctggcgc agatcttgcg tttctgacta
      481 aaggacacaa tatgatttat cctgatgaag caatgcttta cgcaccggtt gaatggcacg
      541 actgctccga aggtttcgag gacattcgtt atgaaaaatc caccgacggt atcgcaaaaa
      601 tcaccattaa tcgtccgcag gtgcgcaatg ccttccgtcc tctgacggta aaagagatga
      661 tccaggcact ggcagatgcg cgttatgacg acaatatcgg cgtgatcatt ctgactggcg
      721 caggcgataa agcgttctgc tccggtggtg accagaaagt gcgtggtgat tacggcggct
      781 ataaagatga ttccggcgta catcacttga acgtgctgga cttccagcgt cagatccgta
      841 cctgtccgaa accggttgtc gcgatggtgg ctggctactc catcggcggt ggtcacgttc
      901 tgcacatgat gtgcgacctg actatcgcag cagataatgc catcttcggt cagactggcc
      961 cgaaagtcgg ttccttcgac ggcggctggg gcgcttccta catggctcgt atcgtcgggc
     1021 agaaaaaagc gcgagaaatc tggttcctgt gccgtcagta cgacgcaaaa caggcgctgg
     1081 atatgggcct tgtcaacacc gtggtaccgc tggcggatct ggaaaaagaa accgtccgtt
     1141 ggtgccgcga aatgctgcaa aacagcccga tggcgctgcg ctgcctgaaa gctgcgctga
     1201 acgccgactg tgacgggcag gcggggctgc aggagctggc gggcaacgcc accatgctgt
     1261 tctacatgac ggaagaaggt caggaaggtc gcaacgcttt caaccagaaa cgtcagcctg
     1321 atttcagcaa atttaaacgg aatccgtaat gcgtagcgcg caggtatacc gctggcagat
     1381 ccccatggac gcgggggttg ttctgcgcga caggcggtta aaaacccgcg acgggctgta
     1441 cgtttgcctg cgtgagggcg agcgcgaagg gtggggggag atctccccac tgccgggctt
     1501 cagccaggaa acctgggaag aggcgcaaag cgtgctgctt gcctgggtaa ataactggct
     1561 ggcaggcgat tgcgagctac cgcagatgcc ttccgttgcc tttggcgtaa gctgtgcatt
     1621 ggcggagctg gcagatacgc tcccgcaggc ggccaattac cgtgcggcac cactgtgtaa
     1681 tggcgatcct gacgatctga tcctcaaact tgctgatatg ccaggcgaga aagtggcgaa
     1741 ggtcaaagtg ggattgtacg aagccgtgcg cgacggcatg gtggttaatc tgttgctgga
     1801 ggcaattccg gatctgcatt tgcgtcttga cgcaaatcgc gcctggacac cgctgaaagg
     1861 ccagcagttt gccaaatatg ttaacccgga ttatcgccac cgcatcgcgt ttctcgaaga
     1921 gccgtgcaaa acccgcgatg attcgcgagc gtttgcccgt gaaaccggca ttgccattgc
     1981 ctgggatgaa agcctgcgcg agccggattt tgcctttgtg gctgaagagg gcgtgcgcgc
     2041 ggtagttatc aaacccacgc tcacgggcag tctggagaaa gtacgcgagc aggtacaggc
     2101 ggcgcacgcg ctggggctga cggcggtgat cagttcttcc attgaatcga gcttaggctt
     2161 aacgcaactg gcgcggattg ccgcctggtt aacgccggac accattccag ggctggacac
     2221 gctggatctg atgcaggcgc agcaggtacg tcgctggccg ggtagcacgc tgcctgtcgt
     2281 ggaagttgat gcactggagc ggttgttatg atcttctctg actggccgtg gcgtcactgg
     2341 cggcaagtgc ggggagaagc catcgcctta cgtcttaatg acgagcaact taactggcgc
     2401 gagctttgtg ctcgcgtcga tgaattagcc tcaggctttg cggcgcaggg cgttgttgag
     2461 ggcagcggcg tgatgttgcg ggcgtggaat acgccgcaaa cgctgctcgc ctggctggcg
     2521 ttactgcaat gcggggcgcg ggtgttgccc gtgaaccctc agctgccgca accgttgctt
     2581 gaagaattgc tgcccaatct gacgctgcaa tttgcgctgg tgccggatgg ggaaaacacg
     2641 tttccggcgt taacgtcgct gcacattcag cgggttgaag gcgcacatgc cgctacgtgg
     2701 cagccgacgc gtctgtgctc aatgacgttg acctcgggtt ctaccgggtt gccgaaagcc
     2761 gctgtccata cttatcaggc ccatcttgcc agtgccgaag gggtgttatc gttgattccg
     2821 tttggcgatc acgatgactg gttgctctct ttaccgctgt ttcacgtctc cggtcaggga
     2881 attatgtggc gctggttata cgctggtgcg cggatgacgg tgcgtgataa acagccattg
     2941 gagcaaatgc tggcaggctg tactcacgct tcactggtgc caacacaact ctggcgtttg
     3001 ctggttaacc gcagttccgt ttccctgaaa gcggtgttac ttggcggcgc ggctatcccg
     3061 gtcgagttga cggaacaggc gcgcgagcag gggattcgtt gcttttgcgg ctatggtctg
     3121 accgagtttg cctccacggt gtgtgcgaaa gaagccgacg gcctggcaga cgttggttcg
     3181 ccgctgccgg gtcgggaagt gaaaatcgtt aataatgaag tgtggctgcg ggctgccagt
     3241 atggcagaag gttactggcg taacgggcaa ctggtttcac tggttaatga cgaaggctgg
     3301 tacgctacgc gcgatcgcgg tgagatgcat aatggcaagc tgaccattgt cggacgttta
     3361 gacaatctgt tcttcagtgg cggtgagggt attcagccgg aagaagtcga gcgtgttatt
     3421 gctgcacatc ctgcggtttt gcaggtgttt atcgttcccg ttgccgacaa ggagtttggt
     3481 catcggccgg tagcggtggt ggagtatgac cagcaaaccg ttgatcttgg cgaatgggtg
     3541 aaagataagc tggctcgttt tcaacaaccg gtgcgctggc tgactttacc gccggagctg
     3601 aaaaacggcg gtattaaaat ttcacgtcag gcgctaaaag agtgggtgca acgtcagcaa
     3661 taattaaccc tccgtttcac ttctcatttc tgccattcca ttgccgttga taaagagttt
     3721 atctgttaat ctgtaaattg atgtgaaaac tcttagcaaa caggataatg caatggaatg
     3781 gctggtcaaa aaatcgtgct gcaataaaca agataacaga catgtactta tgctctgcga
     3841 tgctggcggc gcgataaaaa tgatcgccga agtgaagagt gacttcgccg tgaaagtggg
     3901 ggatttactc tcgcctttgc agaatgcgct ttattgtatt aatcgtgaaa agctgcatac
     3961 ggtgaaagtc ctctcggcaa gcagttatag ccccgatgag tgggaacggc agtgtaaagt
     4021 ggcagggaaa actcagtaac gttgttttgt cgtgggcagg aaaatcagca tcaacccgct
     4081 cataatacag gctactccca gtagtgcttt cagcgaaaaa gtcccttccc agccgggaag
     4141 taccattgaa gcaatccata ccagcacata actcatactg agcaaggcat aggctttgct
     4201 tagcgcaagt ttatgaagcg ttttatacca acaaaaaacg gaaagcagat aacccagcag
     4261 cccgagtaac agtatccggg catccaggcc aaaagctaac agagccgcga taaaatccca
     4321 taagtgcgtc atcggcggca gatgactcgc cgcaaaacct aggcttaatt gcgcaactga
     4381 ggcaataatg acgctgaata atccccacat caggcccatt acaccgtact cccgaggatc
     4441 acaatgccac caataatgaa cgctacccca caccagtgac gcggcgatac cggttcgtgc
     4501 cacagtttta ctgcagccag cgtcacccag acaaaattca gacttaacat cgggtaggca
     4561 atgcctaccg gtacgttctg caagaccagc agccagagca ccatggcaag accaagacaa
     4621 gccagcgcca gtcccagcca cagcacgata tgtttgcgcc gtttgtttat cgccacaaag
     4681 caggttgcct gtttctgaca caactgcccg gcaacgctaa gcaagctggc aaagactaat
     4741 gttagccaga tcatttggga cgatactgaa tcagcaccag acgctcctga cgatcgatgg
     4801 catctgcggg cggaatggcg agactgttga tatcttcatc gcggtcaacc gaaagcacga
     4861 gagtaataat cccctcctga cgatgttgat taagccagtt tgcgaactca tcaccgctga
     4921 caaatctccc tttcgcatcc ggataattaa ggccgtattt caactcacct gtctggcgat
     4981 acatgatgat gtcatcgcgt tgcaggctcc atgccagacc tgcggcaaca ccgacgctat
     5041 cagtaagaat atagcggctt ggctgaagtg attcttgtgt catctcgaca aaaaattgcg
     5101 gatgttttcc ttccataact ctgtcaggaa ttgaaaatcc taccagcaac gccagcccca
     5161 gcgggcaaag cgcggcaaaa ggccaggtct tttcgacgtt tgttaaggtg taccagccga
     5221 aaaatgccca tagcgaaaaa atcgaccagg cacaaaagac tttatagctc tcgaaggttt
     5281 gccacaccgg cgtgttcatt ggtccccacg gggagaccac aaatgtggca ataatgccag
     5341 tgacgccaaa agcgatgtta atccagccat taatccgcag cgccagagga ttattttttg
     5401 ctgccagcaa agcgtaatgc gccatcagca ttgccagaga tgcaaagcag gaaagaatat
     5461 aggtgggcaa tttaccttta gcgacggaga aaaacagcag cggcattatc gtccagctca
     5521 acaaatagac ggttgcggaa tgcttgcggt ttttccagcc tgtgtacagt gcaccgggga
     5581 gtaatcccag ccacggcagg ctaccggcaa tgatgaccgg cacgtagtac cagaacggag
     5641 ctctatgttg ggcatcatcc agtgcaaagc gttgaatatg ctcaacccag aaaaaatagt
     5701 gccagaagtc aggctcccgc tgcgctatcg ccagtcccca agggagaacc gtcagtacgc
     5761 aactgataac cgccagccag ccgtaaataa agagatcttt ccagcgtttt tgcgtcgcta
     5821 cccatggcag cacgcttaat accggcacgg caagggcgag aaaacctttg gtcatcaccc
     5881 ccatgccgca ggtgattccc agcagtaaaa atcctgcgct tttgcctttc cacgtctgtg
     5941 cctgcattgc cagccagaag ctgcacattc ccgccaccag ccagaatgca ataaacggat
     6001 cgagcacggc ataagtgccg atggcataga caataaacaa tgagagataa attactgtgg
     6061 cgagtagagc cagacgttta tcgcgccata agcgcagagt aaaccaggtc accagcgcgg
     6121 cagttaacag ggtcgcaaag ataacgcctg cccgcacacc aaagttattc gcgccaaata
     6181 gccattgccc aatgctgtta atccagtatc cggcaatggg tttttcgaaa taacgtagcc
     6241 ctaacagatg gggcacaatc cagtcgccgg atgccagcat ttctcgactg atttccgcat
     6301 aacgcgtttc atcgggttgc cagagcagac gcgtgctgat cggtaacagg taatagcagg
     6361 caataaacgc gaagaggccg ataaggtaac gtaccgattt catcagcgac tacccgcaat
     6421 ttgttggcaa cccagccagc cttcacgtcc ggcaatattt ccgcgaacaa cttgtccgag
     6481 cggcaacgtc tctgataaca gttcgcttaa agggcaaaat gtcacgcctt cctgagctgc
     6541 gcgtttgagg agatccacaa aattatgctg ataagcgcag ccttcaactt ctgcatgaat
     6601 ggtataaacc ggcgtgcctt tatctcgcag gatgcggttg agtaaccaac cgttaaaatc
     6661 ttctgctttc acatcccgac caatcacttc atcccaggtg ggtaaggtca ccggaatttg
     6721 cgcagtgcca gggtttcctg attcgagcaa tggacggaac ggcatggccc cacgacaatc
     6781 gctgttgtag cgcaaatgga acgcttcttt tgcttcgatc accttctggt cggcacgcca
     6841 gcccgcagcg gcagaacagg ttaccggttg accgataatc tcttccagag tgcgaagacc
     6901 gcgtgcaata tcgtcgatca ttgtttgccg atcccagtta ccgctacggg cttgccaggc
     6961 atggtgatcc caggcgtgca ggccgacttc gtgatgttta gccgcttcac gaatgatatc
     7021 ggcattggca tgaccaatct ctttacctgg ccaggccgta cctgccagta aaatatccca
     7081 gccataaagc gatgccgcat ttgagcgcag catcttccac aaaaactgtg gcttcaccag
     7141 tcgccagaga tggcggccca tattgtccgg gccgacgctg aaaaaaatgc tggcctgaat
     7201 attatgctta ctcaagattt ccagcagacg cggcacgcct tcacgggtgc cacgaaaggt
     7261 atcgacatca atgcgtaagc ctactttggt catgatggtt tatccgtaag atcaacggtg
     7321 cgcaggaaga aatccagcgt ttcgtcgatg gtttcctgca tatcaatttt cggctcccag
     7381 tccaggcagc ggtgggcatt gcggatgctc ggtttacgat gctctacgtc ctgatatcct
     7441 ttgccgtagt agctgctact ttcgacaacg cgaaagcccg caaacggtgg gaaatgatgg
     7501 cgcagcggat gtttttcgaa gctcgccagc agcatctcgc ccagttcctc aatgctcgct
     7561 tcgttctcag gattgccaat gttgataatt tcaccgtcgc agcgatttcc cgcattttcg
     7621 ataatgcggt ataacgcctc gataccatcg cgaatatcag taaagcagcg tttttgtttt
     7681 ccgccatcaa tcagcttaat cggtgaacct tctaccagat tgagaatgag ttgcgtaata
     7741 gcgcgggagc tgccgattcg tgccgcatta aggttatcca gtcgcggtcc catccagtta
     7801 aacgggcgga agagggtgaa ctgtaaaccc tctttttcgc cataggccca gatcacccga
     7861 tcaagtaatt gttttgatac cgaataaatc cagcgtggtt tattcaccgg gccgacgatt
     7921 aaattagaat ggtcctcatc gaagtattta tcgctacaca tcccataaac ttctgaagtt
     7981 gacgggaaga tgattcgctt acggtacttc acgcagtagc ggataatgcg cagattctct
     8041 tcaaaatcga gttcaaatac gcgcagcggg ttgcgggtat attcaatcgg cgtggctatc
     8101 gccaccagcg gcaagacgac atcacatttt ttgacatgat actcaatcca ttcggaatga
     8161 atactgatat cgccttcaac aaagtgaaaa tgcggatgat tcagaaaacg gcttatcgca
     8221 tcgctgccaa tatccagacc gtaaacttca taatgatctt cgcgcagcag gcgttctgtc
     8281 agatggttgc caataaagcc attcaccccg aggatgagta cccgggtacg gcgtcgggcg
     8341 gtgcaggcag gctggctatt caagcgtgaa ccttgcacca ggcccagcgt ctgcgctaat
     8401 tgcgagccct gcatagtaat gccgtcgccc gcctgtccgg tgacgatttc cagcgcgcca
     8461 tcgccacagg caatcagcag tggcgcaaca gaaatcacgc tccccggctg tgctttgctg
     8521 gcatgaggat gaacacgcga cgaccagacg gtgaatttct gattgccaac atagctgaag
     8581 gcacccggcc acggatcggc aacggcacgt accatgttgt gcagtacgga tgccggttta
     8641 tgccattcaa ggaaactgtc atccggcgtt ctgcgaccaa aacaggtggc ttcgttttcg
     8701 cgctgggcga tttccagaat attaccgtgt ttaatggcgg gtaatgtctg ttccagtagc
     8761 tggcgcgcgg catggcacaa tttatgatgc agcgtaatag cgatatcgtc tggcgcaatg
     8821 gcaatgcgca gttgcgccac aatggccccg gcatcggcac gtttcaccat tcggtgcaat
     8881 gtaacgccag tttccgtttc accgttgacc agcacccagt tcagcggcgc gcgaccacga
     8941 tattttggta acagcgaacc atgcagatta aatgcacctg cgggagcgag ctgcaaaatt
     9001 tcgtcgcaaa taagatggcg ataataaaaa gagaaaatca catctggcga cagttgggca
     9061 atgcgttcca cccacagcgg atgattaacg tcatccgtcg cataaaccgg aatgcctctt
     9121 tccgctgcca gacgagccac cgaaccataa aaggcttttt caccgggatt atcggtatgg
     9181 gtaaaaatgg cgctaatttc gtaaccggca gccagcagag cttcaatacc gaggcatccc
     9241 atatcgtggt aggcaaaaac gacggttttc attattcatt ttccttgctg gatggacgga
     9301 taacttgctg aacaaaatag cgggggcggg cgcggacatc ggtgtagatc ctgccgatat
     9361 attcaccgag taatcccatg ccgataaact gagcgccaat aaaagtaaac agcacggcaa
     9421 atagcataaa gacgccttct gccgcccatt gtggtccgaa ggtcaggcgt aaaatcacca
     9481 gcagcaccgc aatgctaaaa cctccaatcg caataatgct gccgagcaga ctcagcatac
     9541 gtagcggcgt tgtggtaagg caggtcacca ggtcgtacat caaattaatc aggcgcataa
     9601 aactgtattt ggattcacca aactcacgct cggcatgatg tactggaatt tcaatggcac
     9661 ggcgggcgaa gatattcgcc agaatcggga taaaggtgct acgttcatgg cagtgcaaca
     9721 tcgcatcgac aatatgacgg cgataggcgc gcagcataca accgtagtca cccatcgctt
     9781 tgccggtggt gcgctgaata agccggttaa tcatcttcga agctgtttta cgaaaccagc
     9841 tgtcctggcg gttctggcgt acagtcccta ccacgtcgta accttcatcg gcttttgcca
     9901 ccaggcgggg gatttcttct ggcggattct ggagatcggc atcaagggta ataattaagt
     9961 cgccggtaac gtgactgaat cccgccataa tcgctgaatg ttgcccgtaa ttgcggttaa
    10021 gcaaaataga cacaatatgg ctgttctccg cttgtgaggc ttcgaccagc atatgcgcgg
    10081 aattatcgct actgccgtca tcaatcagca ggatctcata ctctttcccc aacgattcac
    10141 aggctgtggt ggtgcgcctg attaattccg gtaagctttc ctgctcgtta taaacgggaa
    10201 taaccaccga gactttctta acagggtgga tttcaaacat ggcttattgt cctgcgagtt
    10261 gctgaagggc tgtgatgaca cggtcggcat cggcggtagt catatccggg aacagcggca
    10321 aagaacagat gcgttcgcta ttccattcgg tattcggtaa cgacagcgtg ggaaaacgct
    10381 cgcgataata tttttgtgtg tgagcggcgc ggaaatgtaa cccggtacca atgcctcttt
    10441 cttttaacgc ttccatcaac gcatcgcgac tgataccaca acgttgttca tcgacacgaa
    10501 taataaacag atgccaggcg tgaacgtgcg gccaggcggg aaggcttaat ggctgaaagg
    10561 ggagagctgc cagtgcttgc tgatattgct gggcaatttc gcgccgacgg gtgttgaggt
    10621 gctctaattt tgctaactgt gtcagggcaa ttgcggcgtt aatatcggtc agattgtact
    10681 tatagcccgg tgttaagact tcagcctgcg gtgcacggcc ccaggtttgt ctgtcatagg
    10741 catcgacacc cagaccgtga aatttcagca tccgtagctg gcgggcaagg ttttcattat
    10801 cagttacaat caggccacct tccgcacagg taatattttt aatggcatga aatgaaaaaa
    10861 tagcggtacc ttttgcgcca atatgtcgcc ctttgtaata cgtaccgacg gcatgggcag
    10921 catcttcgat aactgcgatg ccgtaacgtt cgccaatggc gcgaatggcg tcaatatcta
    10981 ctggcgcacc ggcataatgc accggaatga tggctttagt gcgtggcgtt atggctgact
    11041 cgatcgcttc aggcgtgacc atcagcgtat cgcggtcgac atccaccatt accggcgttg
    11101 cacccaacaa ggaaatcata ttgagggttg aaacccaggt cagggaaggc gtaatcactt
    11161 catcgccctt gccaatttcc aacgccatta gcgtgatatg cattccggcg gtggctgaac
    11221 tgaccgcgat ggcatgctga tttcccgtca actggcaaaa ggcttgctca agcgcctgat
    11281 tcttcggacc ggttgtgatc caaccggatt cgagaacctc tttaactgca gcgagttcct
    11341 ccacgcccat tgctggtcgc gaaaaaggca aaaattctga cattgctttt ccttccgcca
    11401 ttgaatacct gtccacttat
//
LOCUS       DAZGXZ010000153        11410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000153 DAZGXZ010000000
VERSION     DAZGXZ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.153"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..400
                     /locus_tag="ACHKJF_004417"
     CDS             14..400
                     /locus_tag="ACHKJF_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951667.1"
                     /translation="MKELEQNEIFSETSPDAINELKEIAERISKICKEYKIDFVFSFS
                     VLTEVGNNEYKDSRLVSCGLNGKAPSPYIHAACEVVRSNIGAQQIHTLAQALEFAREN
                     SECDCPECQHEKGKTTHKTANQATFH"
     gene            508..2991
                     /locus_tag="ACHKJF_004418"
     CDS             508..2991
                     /locus_tag="ACHKJF_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0951668.1"
                     /translation="MSADKQTFALHCEAKNDKVRKRLGIKGGFFWADARKLSVAVSRC
                     IAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMMAIPFN
                     DNINGEAAVTASNDNDEQSGAQPENAEKNKSQDSNDDCHECEIPVATLSLTHRFLHLY
                     FFGKEEGGKYRHHATPEQRNTVIRMEMDMEDSNLQSLLTAVRSHPELDKLTNHHLSRL
                     AESVSRAFTHAATRRISPAEFDKFISIWMKTDYLDQGLLTKEWAKGNYVSEINRTPSG
                     ANAGGGILTDRGEGFVHDLTSLERDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENK
                     PPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNRVLTETDHATP
                     DPMIVAIACGRSSAPMPQRVTEEGKKDDEEKPQPSDAMADEQATAETVEPDTTEHHQD
                     TQPLDAQSQIDTKYEKLRAELHEARKNILPKNPVDADKVLAASRGEFVEWISDQNDPK
                     WVKGIETRDSVNQNQQETEQNGQKAEQNSPNALQNEPEAQPETEKACDSCGQTGGGNC
                     PDCGAVMGDATYQDTFNEENLDEYQEKNPEKQESPEHPCKENADGDQHHDSDNETGET
                     ANPLIKVNGHNEVTSTSRLWHHLMIDLETMGKNPDAPVNAIAGKFFDPVTGEMGPEFS
                     KTIDLETAGGVIDRDTIKWWLKQSREAQSAILTNEIPLDDALLQFREFIAENSGEFFV
                     QVWGNGANFDNAILRRSYERQGIPCPWRYNNDRDVRTMVALGLVIDFDARTTIPFEGE
                     RHNALHDARYQAKYVSAIWQKLIPSQADF"
     gene            3049..3303
                     /gene="xisR"
                     /locus_tag="ACHKJF_004419"
     CDS             3049..3303
                     /gene="xisR"
                     /locus_tag="ACHKJF_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0951669.1"
                     /translation="MQQIIYQITPSEWCAESILIAATGLKSGTIERARRTSWMLGREY
                     IHFSVDGEPKPNSECLYNIDAIMRWIKNQKQPGAKNASSG"
     gene            3337..4623
                     /locus_tag="ACHKJF_004420"
     CDS             3337..4623
                     /locus_tag="ACHKJF_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0951670.1"
                     /translation="MSNTSYPTGVENHGGSLRIWFHYNGKRVRENLGIPDTAKNRKIA
                     GELRTSVCFAIRMGNFDYAAQFPESPNLKHFGLGKREITIKALAEKWLDLKKIEIGGN
                     ALNRYQSVVRNMLPRIGENRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPVKGRSVV
                     TVNYYMTTIAGMFQFAADNGYTESNPFNGLTPLKRSRTEPDPLTHDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELISLAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDTLKSQAEMTRFGTQYQIDVKFREFGRTVRHECTFVFNPQLVKKCQQAGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMKDNNIGQIELLNKELTESVPYMPHRARR"
     gene            4589..5404
                     /locus_tag="ACHKJF_004421"
     CDS             4589..5404
                     /locus_tag="ACHKJF_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP0951671.1"
                     /translation="MSHTCPTERDDEIISFSIIELDYYSRHVNCVEGNTTLYALPKPE
                     VVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLP
                     ATFDPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILD
                     SRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQK
                     LAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5457..5852
                     /locus_tag="ACHKJF_004422"
     CDS             5457..5852
                     /locus_tag="ACHKJF_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP0951672.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            5893..6636
                     /gene="cmoA"
                     /locus_tag="ACHKJF_004423"
     CDS             5893..6636
                     /gene="cmoA"
                     /locus_tag="ACHKJF_004423"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP0951673.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            6633..7604
                     /gene="cmoB"
                     /locus_tag="ACHKJF_004424"
     CDS             6633..7604
                     /gene="cmoB"
                     /locus_tag="ACHKJF_004424"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP0951674.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(7769..10198)
                     /gene="torZ"
                     /locus_tag="ACHKJF_004425"
     CDS             complement(7769..10198)
                     /gene="torZ"
                     /locus_tag="ACHKJF_004425"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP0951675.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTIGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNVFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(10223..11323)
                     /locus_tag="ACHKJF_004426"
     CDS             complement(10223..11323)
                     /locus_tag="ACHKJF_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP0951676.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2991 a         2862 c         2719 g         2838 t
ORIGIN      
        1 cggaaattga aaaatgaaag aacttgagca aaatgaaata ttttccgaaa ccagtcctga
       61 tgcaataaat gaactcaaag aaattgcaga acgcatcagt aaaatatgca aagaatacaa
      121 aatcgacttt gtattttctt tttcagtgct tacagaagtt ggaaataacg aatataaaga
      181 cagtcgtttg gtttcatgtg ggttaaatgg caaagcacca agcccatata ttcatgctgc
      241 atgtgaagtc gtcagaagca acattggagc acaacaaatc catacactgg cgcaggctct
      301 tgagtttgca agagaaaatt ctgagtgcga ctgccctgaa tgccagcacg aaaagggaaa
      361 aacaactcac aaaacagcaa accaggcaac cttccactga aataaaaatc cggcagcgca
      421 ggctaccgga tttctccctg cgtcaccgta ttcggagaaa tcagacaaag ggccgctaat
      481 tctaatccag ccagaggttt aaatacaatg agcgctgata aacagacttt tgcactacac
      541 tgcgaagcaa aaaacgataa agtcagaaaa cgccttggca tcaaaggcgg ttttttctgg
      601 gctgatgcca gaaaactttc tgtcgcagtt tcccgctgca ttgcagccat ggacgatgca
      661 ggctacgacg aggatgattt caaaaaaccc gttcgcgtaa atttccccgt cgtgaatgac
      721 cttccaccgg aaggcgtgtt tgatactgaa ttctgcaacc gctatgaaaa aggcggggaa
      781 gatggtatca ccatgatggc tatacccttc aatgacaaca tcaacggcga agctgctgta
      841 acagccagca atgacaacga tgaacaaagc ggtgctcagc cagagaacgc agagaaaaat
      901 aaatcccagg acagtaacga tgactgccat gaatgtgaaa ttcccgtcgc cactctcagt
      961 ctgactcatc gtttcctgca tctctacttt ttcggcaagg aagaaggcgg aaaatacagg
     1021 caccacgcaa caccggaaca acgcaataca gtgatccgca tggaaatgga tatggaggac
     1081 agtaacctcc agagcctcct cacagctgta cgctctcatc ctgaactgga taagctgaca
     1141 aaccaccatc tgagcagact ggcggagtcg gtatccagag cattcactca cgcagcaacc
     1201 cgtcgtatca gcccggcaga attcgacaag ttcatatcca tctggatgaa aactgattat
     1261 ctggatcagg gattactgac aaaagagtgg gcgaagggaa attatgtatc tgaaatcaat
     1321 cgcaccccat caggtgctaa tgctggcggt ggaattctta ccgatcgcgg cgaaggtttc
     1381 gtccacgatc tgacgtcact ggagcgcgat gtggccacag gtgtactggc tcgttcaatg
     1441 gacgtggaca tttataacct tcatccggca cacgcgaaac gcattgagga aattatcgct
     1501 gaaaataaac cgccattttc tgttttccgc gacaaattca tcgccatgcc tggtgggctg
     1561 gattattccc gcgccatcgt ggttgcgtcc gtgaaagaag caccaattgg tatcgaggtc
     1621 atccccgcgc acgtcactga atatctgaac agggtactga ctgaaacaga ccacgctacc
     1681 cccgacccga tgattgtggc cattgcctgc ggtcgctcct ctgccccgat gccacagcgt
     1741 gtaacagaag aaggaaaaaa ggatgatgaa gaaaaacctc aaccatctga cgcaatggca
     1801 gatgagcagg caacggctga aacagtggaa ccggacacaa ctgaacatca tcaggacacg
     1861 cagccgctgg atgctcagtc acagatagat acgaagtacg agaagttacg ggccgaactc
     1921 cacgaggcac ggaaaaacat tctgcccaaa aatcctgtcg atgcagacaa agtactggct
     1981 gcctctcgcg gagagtttgt tgaatggatt agcgaccaaa atgatccgaa atgggttaag
     2041 gggattgaaa cccgcgattc tgtgaaccag aaccagcaag aaacggaaca gaacggccag
     2101 aaagcggaac aaaacagccc aaatgcgtta caaaacgagc cagaagcaca accggaaacc
     2161 gaaaaagcct gcgattcctg cggccagact ggtgggggta actgccctga ctgtggcgcg
     2221 gtgatgggcg atgcaacgta tcaggacacc tttaatgaag aaaacctgga tgaatatcag
     2281 gaaaaaaatc cggagaaaca ggaaagccct gaacatccgt gcaaggagaa tgctgacggt
     2341 gatcagcatc acgatagcga taatgaaact ggcgagacag caaatccctt aattaaggtg
     2401 aacggtcata atgaagtcac atccaccagc aggttgtggc accatctgat gattgacctt
     2461 gaaacaatgg gaaaaaatcc tgatgcgcca gtcaacgcta tagccggtaa gttttttgat
     2521 ccggtaaccg gagagatggg accagaattc agcaaaacta tcgatctgga aaccgcaggc
     2581 ggagtcatcg atcgggacac cattaagtgg tggctgaaac agtcacgcga agcacaatcc
     2641 gccattctta ccaatgaaat cccgttggat gacgcgctgt tgcagttcag ggagtttatc
     2701 gccgaaaact ccggtgaatt ttttgttcag gtgtggggaa atggagccaa cttcgacaac
     2761 gcaattttac gccgttcata tgaacgacag gggatcccct gtccgtggcg ctacaacaac
     2821 gatcgtgatg tacgaacgat ggttgctctg ggactggtga tagatttcga tgccagaacg
     2881 actattccat tcgaaggtga acgccataac gccctgcacg atgcgcgtta ccaggcaaaa
     2941 tacgtgtcag caatctggca aaaactgatc ccgagtcaag ctgattttta atgttcaacc
     3001 ctgatcgccg tccaccagct atagtggcgg cggttgcaag gcaattctat gcaacaaatt
     3061 atctatcaga taacacccag tgaatggtgc gctgaaagca tcttaattgc tgcaacagga
     3121 ttaaaatcag gcactatcga gcgagcacgt agaacatcat ggatgttagg aagggaatat
     3181 atccacttct ctgtggatgg agagccaaaa ccaaacagtg aatgcctgta caacatagat
     3241 gcaattatgc ggtggataaa aaaccagaaa caaccaggtg ccaaaaatgc aagttccggt
     3301 taacctgtta atgctcctgg acgtctggga ggtttaatga gtaacacatc atacccaaca
     3361 ggcgttgaaa accatggcgg ctcactccgc atatggtttc actataacgg caaacgtgtc
     3421 agagaaaacc tcggtattcc tgacacagcc aaaaaccgga agattgcagg cgaactcagg
     3481 acttcagttt gttttgcaat cagaatgggg aatttcgact acgctgcgca gttccctgag
     3541 tctccaaatc tgaaacactt cggtctgggg aaaagggaga taaccattaa ggcacttgcc
     3601 gaaaaatggc tggatctgaa gaaaatcgag ataggtggta acgcgctcaa tcggtatcaa
     3661 tctgtggtga gaaacatgct ccctcgtata ggggaaaatc gactggcttc atctgtaaca
     3721 aaagaagatt tactctttat caggaaagat ttattgaccg ggtatcacaa tctctcgaac
     3781 ggcaagacaa ctcctgtcaa agggcgatct gtagttacag ttaattacta catgacgaca
     3841 attgcaggta tgtttcaatt tgcagctgac aacggataca cagaatcaaa cccgtttaac
     3901 ggtctgacac cattaaaaag atcaagaaca gaaccagatc cgctcacaca tgacgaattc
     3961 attcgtttta ttgatgcttg ccaccatcaa caaacaaaaa acctgtggtc tttagcagta
     4021 tacacaggca ttcgtcatgg tgagctgata tctcttgcct gggaggatat tgatttaaaa
     4081 gctaaaacaa tgactatccg tcgtaattat acaaaactcg gtgagttcac tctaccaaaa
     4141 accgaagcgg gaacagatcg tgttattcat cttgttcaac cagccgttga taccctgaaa
     4201 agtcaggctg aaatgacaag atttggtact caatatcaaa ttgacgtcaa atttcgggag
     4261 ttcggacgca ccgtaagaca tgaatgcaca tttgttttta atccgcaact ggtgaaaaaa
     4321 tgccagcagg caggccacca ctacaaagca gactccatca gagactcatg ggcatcggca
     4381 ttgagacggg cgggactacg gcacaggaaa gcctatcagt ccaggcatac ttatgcctgc
     4441 tgggcattat cggcgggagc taatccaagc tttatcgcaa accagatggg acatgcaaac
     4501 gcccaaatgg ttttcaatgt ttacggagca tggatgaaag ataacaatat cgggcagata
     4561 gaactgctca ataaggagtt aactgaaagt gtcccataca tgccccacag agcgagacga
     4621 tgaaataatc tctttcagta tcattgagtt agactattac tcccgtcatg ttaactgcgt
     4681 ggagggcaac accacgcttt acgccctgcc gaaacccgag gttgtcctgc gctggcgtga
     4741 acagaccaca gatgacttcc gcttctgttt taagtttccg gcgaccattt cgcatcaggc
     4801 agcattacga cattgcgatg atttagtgac tgaatttttg acccgcatgt caccgttggc
     4861 tccgcgcatt gggcaatact ggctgcaact gcctgccaca ttcgacccac gggagctgcc
     4921 tgcgctttgg cattttctcg attctctccc tggcgaattt aattatggtg tggaagtccg
     4981 ccatccacag tttttcgcca aaggggaaga ggaacaaacg cttaatcgcg gtttacatca
     5041 gcgcggcgtt aatcgggtga ttttagacag ccgcccggtt catgcagcac gtccacacag
     5101 tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg ctgtactgac
     5161 ggcgacaaat ccgctgatcc gttttatcgg gagtgatgat atgacgcaaa accgggaatt
     5221 atttcaggtc tggttacaga aattagcgca gtggcatcag accactacgc cttatctttt
     5281 tttacatacg ccagacattg cccaggcccc ggaactggta cataccctgt gggaagactt
     5341 acgtaaaacg cttccagaga tcggagcagt tccggctatt ccacagcaat cttctctttt
     5401 ctgaatttgc cacctatcat agacaggtgc catcggccat tttaaaggga gtttgtatgg
     5461 taagcgcgct gtatgccgtt ttaagtgcgt tgttattaat gaagttctct tttgatgtcg
     5521 ttcgcttgcg tttgcagtac cgcgttgcct atggtgacgg tggttttagc gaactgcaaa
     5581 gcgctattcg cattcatggt aacgcggtgg aatatattcc catcgcgatt gtgctgatgc
     5641 tgtttatgga aatgaatggc gcagaaacct ggatggtgca tatttgcggc atcgttttgc
     5701 ttgctggtcg tctgatgcat tattacggtt ttcatcaccg tctgttccgc tggcgacgtt
     5761 ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat ctttggtata
     5821 tgccctggga gttggttttc tccctgcgtt agcgcacaat acgccacttt ctttttcccg
     5881 gatttttacg ttatgtctca ccgcgacacg ctattttctg cccctatcgc cagactgggc
     5941 gactggacct ttgatgaacg ggtagctgaa gtcttcccgg atatgatcca gcgttccgtt
     6001 cccggctatt ccaatattat ttccatgatt ggtatgttag ccgagcgctt cgttcaacct
     6061 ggtacgcagg tttacgatct gggttgttct ctgggcgcgg cgacgctctc ggtgcgtcgc
     6121 aacattcatc atgataattg caaaattatt gccatcgaca actccccggc gatgattgaa
     6181 cgctgccgtc gtcatattga cgcctataaa gcccctacgc cagtagacgt cattgaaggt
     6241 gatattcgcg atatcgccat tgaaaacgca tcgatggtgg tgctgaattt taccctgcaa
     6301 ttcctggaac cttccgagcg ccaggcatta ctggataaaa tttatcaagg actgaacccg
     6361 ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag atgccaaagt tggtgaactg
     6421 ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt acagcgaact ggagatcagc
     6481 cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac ccataaagca
     6541 cgcctgcata aagccggttt tgagcatagc gagctgtggt tccagtgctt taactttggt
     6601 tcactggtgg cattaaaagc agaggacgct gcatgatcga ctttggtaac ttttattctc
     6661 tgattgccaa aaatcatctt tcacactggc tcgaaacgct gcccgcgcag attgctaact
     6721 ggcagcgcga gcagcagcac gggctgttta agcagtggtc caacgcggtg gaatttctgc
     6781 ctgaaattaa accgtatcgt ctggatttat tgcatagcgt aaccgccgaa agcgaagagc
     6841 cactgagcgc cgggcagatt aaacgcattg aaacgcttat gcgcaacctg atgccatggc
     6901 gcaaagggcc gttctcactg tatggcgtca acatcgatac cgaatggcgt tccgactgga
     6961 aatgggatcg cgttctgccc catctttctg atttaaccgg gcgcaccatt ctggatgtcg
     7021 gctgcggcag cggttatcac atgtggcgca tgattggcgc aggcgcgcac ctcgcggtgg
     7081 ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa ctactgggta
     7141 acgatcagcg cgcacatttg ttaccgttag gtattgaaca gcttccggca ctgaaagcct
     7201 ttgataccgt cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg gagcatctct
     7261 ggcagttgaa agatcaactg gtgaatgagg gtgaactggt gctggaaacg ctggttattg
     7321 atggcgatga gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg cgtaatgtct
     7381 atttcatccc ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt tttgtagata
     7441 tccgcattgt ggatgtgtgc gttaccacta ctgaagagca gcgacgcact gaatggatgg
     7501 ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc gagtaagaca gttgaaggtt
     7561 atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct ggtattactg
     7621 ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct tgctaattca atctattgca
     7681 gggactatgt aggcctgata agcatagcgc atcaggcagt tttacgtttg cataacctca
     7741 gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac agcaggctta tcaaatgccg
     7801 ttaacttcgg tgcgttaccc gtatattttt cgatatacac cagcgcagag ttaccggcac
     7861 aggcatttgc cagctgcgag ctggggatat ccgccgttaa cacgttcgca ctgccgtttt
     7921 tacataagcc attttccaga tctggccatg caccttcatg cacgcatacc acgccttttt
     7981 tgatcccgtc agtcaccacc gcacctgtta gtatctgacc acgtttgttc cagacacgca
     8041 ccagatcgcc attcgcaata ccaaatcgag cagcatcttc ggtgtgaata gtgatcggtt
     8101 cacgatcggc aaccgcatat tttttacgca gttccgcata gttaagctga ctgtgtaaac
     8161 ggtgtgccgg atgtgcggtc agaagctgca actgcttctc gtcggcggta cccttccact
     8221 catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat ttttccagcg
     8281 ttttggagta aatctcaatt ttgccacttg gcgtacccag cgcatttttc accggatcgg
     8341 cgcggaaatc accataacga acgtactgtt cgttcttctc gctgcggcgc atttcgatca
     8401 gtttattttg ctgccagaag acattaaaca ttggcatagt gacgcgttgc gcacgggccc
     8461 ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta ccttcggtat
     8521 agatctcttt tccgccaggt ttgagtaatt ccgcaagatc ggcaaacaca tcaaaatcgt
     8581 ttcgcgcttc aaattgcgga gcaacagcct gcttcatcgg cacaatatgc tggttgctgt
     8641 aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt aatacgatat
     8701 ctgcatgttt agcagctgcg gtccagtagc attcagaaac gacgatcatc tccggtttct
     8761 gccatgcttt aatcagacga ttagtatcct ggtgatgggt aaagttaccg ccacccgccc
     8821 accagatcat tttgatattc ggatacgttt gctctttacc gttatgctga tatttgccgc
     8881 caggattttc cagcgcatcg acgatacggg caacaggaat cgccctcatt ccgccatcat
     8941 cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc acgccgccaa
     9001 cacgtgtcgg gttgccacca tttgagtaat gataagagaa accaaaaccg ccgcccggtg
     9061 taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc tgttcaccat
     9121 attgctggcg ctgaattccc cagcctgcca tcagcattgt gcgattcgcc gccatcagtt
     9181 cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgcc caggccgcgc
     9241 tcttcggcgt attatcgctc ttacctctca gatactcttc aaactgcgga tacccgatag
     9301 tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt catcagggta tgcgcaatcc
     9361 ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca ttatcgccaa
     9421 agaattcgat ggtttcggag cggataggat caatggcaat cactggtttg ccagattttt
     9481 tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca atttttaagg
     9541 tattcagcgg gttcattccc cacagcacca caacctggct gttttccaga atcagcggcc
     9601 aggaggtctg ctgttcatac acctctacag aaccgaccac atgcggcatg atcacctgtg
     9661 ccgccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg ttcatataac
     9721 gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg tatgatccgg
     9781 caaaaatagc cgatggtccg ttagctttgc gaatgcgatc gtgttgctca tgaatcagtt
     9841 ttaatgcttg ttcccagctc acctgtacat aggtatcttc gccacgacct ttcgtcggtt
     9901 gcagtggatt atcgagatag ctttttctca ccatcggatg ctgaatacgc gtcgtggtgt
     9961 gtacctgatc cgccgccgta gactgtaagg aattcggtat ggttttcgcc agcgcgcctg
    10021 ttgaagaaac aatcttgccg tccttcactt ctacgttcat cgctccccaa cgtcccgcgg
    10081 tgaggatttt accgcccttc tcttctgccc atgcggggag cggtgctgcc gatgtcacca
    10141 ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt aatgtcataa
    10201 cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg ttgtactgga
    10261 aataccgcgt taaaatgtcc agttcgtttt cgctcatgct ggttcgcgca cccattcctt
    10321 tggcaatgga cggccatgca ttgacggtgt aatggtcagc ggcaataggc gcatggcaac
    10381 cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcggttta cggtccgcta
    10441 atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg ccgtattcgt
    10501 cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt aatgtggcta
    10561 gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc atttgataac
    10621 cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga tcggtaccag
    10681 gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata tgtgcgccat
    10741 tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca tccattttta
    10801 tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg ccgccctttt
    10861 gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc atggcatcaa
    10921 aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc catactgttt
    10981 cggccatttc ctggcgatga gtttcgaact tatcgtcact gtctattttg ccgctaacaa
    11041 attcatgata aatatcttta gatgctttta atttagcaaa taaataatcc atccctgact
    11101 ttggaatatg gcaatctgca cattccgcac gtatcccttt ctggttcgaa aagtggacag
    11161 ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa aatgctgtat
    11221 ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc gccgccacca accacaaccg
    11281 ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg catagattcc tctaaataaa
    11341 ttccagaaca ccgaaatcat tgtataatta aatacacaac gaattaaccg cttgcataac
    11401 tcggcacaac
//
LOCUS       DAZGXZ010000154        11305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000154 DAZGXZ010000000
VERSION     DAZGXZ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.154"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            137..271
                     /locus_tag="ACHKJF_004427"
     CDS             137..271
                     /locus_tag="ACHKJF_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0951677.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            250..354
                     /gene="speFL"
                     /locus_tag="ACHKJF_004428"
     CDS             250..354
                     /gene="speFL"
                     /locus_tag="ACHKJF_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP0951678.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            665..2863
                     /gene="speF"
                     /locus_tag="ACHKJF_004429"
     CDS             665..2863
                     /gene="speF"
                     /locus_tag="ACHKJF_004429"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP0951679.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGDIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2860..4179
                     /gene="potE"
                     /locus_tag="ACHKJF_004430"
     CDS             2860..4179
                     /gene="potE"
                     /locus_tag="ACHKJF_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP0951680.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4235..5875)
                     /gene="pgm"
                     /locus_tag="ACHKJF_004431"
     CDS             complement(4235..5875)
                     /gene="pgm"
                     /locus_tag="ACHKJF_004431"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP0951681.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILLHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     VATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5901..6446)
                     /gene="seqA"
                     /locus_tag="ACHKJF_004432"
     CDS             complement(5901..6446)
                     /gene="seqA"
                     /locus_tag="ACHKJF_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP0951682.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6631..7395
                     /gene="ybfF"
                     /locus_tag="ACHKJF_004433"
     CDS             6631..7395
                     /gene="ybfF"
                     /locus_tag="ACHKJF_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0951683.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7535..7828
                     /gene="ybfE"
                     /locus_tag="ACHKJF_004434"
     CDS             7535..7828
                     /gene="ybfE"
                     /locus_tag="ACHKJF_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP0951684.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7968..8498
                     /gene="fldA"
                     /locus_tag="ACHKJF_004435"
     CDS             7968..8498
                     /gene="fldA"
                     /locus_tag="ACHKJF_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP0951685.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8708..8794
                     /gene="uof"
                     /locus_tag="ACHKJF_004436"
     CDS             8708..8794
                     /gene="uof"
                     /locus_tag="ACHKJF_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP0951686.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8787..9233
                     /gene="fur"
                     /locus_tag="ACHKJF_004437"
     CDS             8787..9233
                     /gene="fur"
                     /locus_tag="ACHKJF_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP0951687.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9317..9643)
                     /gene="chiQ"
                     /locus_tag="ACHKJF_004438"
     CDS             complement(9317..9643)
                     /gene="chiQ"
                     /locus_tag="ACHKJF_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP0951688.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9693..11099)
                     /gene="chiP"
                     /locus_tag="ACHKJF_004439"
     CDS             complement(9693..11099)
                     /gene="chiP"
                     /locus_tag="ACHKJF_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP0951689.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2758 a         2777 c         2792 g         2978 t
ORIGIN      
        1 ataattatta atttattatt aaacctgcca aaaatattat tatttggcag gtttaatttc
       61 ttaacgcaaa tataaaacac aaaattacaa catttaaata acaaaacagc atctaatatg
      121 gcgctattca taattaatga tttattattt ggggtatgat cgttttttgt tgatcttctt
      181 cacagattat agccatttca tggatagaat aactctacct tcaactgaca cagcaagagg
      241 taaaggtaaa tggaaaataa taaccgctta atgcctcata taaggcggac aacccatatc
      301 atgaagtttg cccatcgtaa tagttttgac tttcatttct ttaatgcccg ctagtcttct
      361 gactaaaggg caccccaacg tacaggtctc cctgacttta agcattacag gttaatacct
      421 gtattcctcg gtgctcatat actgctaacc cttttaaact ctaaataatt cgagtcgcag
      481 cacttgcaac ttgaggtatg acgagtatag ccagttaccg ggctggtctg ggttattgca
      541 tctgcaaaaa gcaaactact gatttattta tcagcggtgg agctttgctt tttttccggc
      601 gtgatcgatt tctcctttga gaaattgagg acctgctatt acctgaaata aagagatgaa
      661 caaaatgtca gaattaaaaa ttgccgttag tcgttcttgc ccggattgtt tttccactca
      721 tcgtgcatgc gtgaatatag acgaaagtaa ttatattgac gttgccgcca ttattttatc
      781 agtcagtgat gttgaacgtg gaaaactcga tgaaatagac gctactggct atgacattcc
      841 tgtttttatt gcaacggaaa atgaagaacg tgtacccgca gaatatctgc cacgtatttc
      901 tggtgtcttc gaacattgcg aatcacgtaa agagttttat ggacgtcagt tagaaaccgc
      961 tgccagccat tatgaaacgc aactgcgccc gcccttcttc cgcgcactgg tcgattatgt
     1021 taatcagggc aatagtgctt ttgactgccc tggtcatcag gggggtgagt ttttccgtcg
     1081 ccatccggca ggcaatcagt tcgtggaata ctttggcgaa atgctcttcc gctctgactt
     1141 gtgtaatgct gacgtagcaa tgggcgatct gctgattcat gaaggtgcgc catgtattgc
     1201 acaacagcat gcggctaaag tctttaatgc cgataaaacc tatttcgttt taaacggtac
     1261 ttcatcttct aataaagtgg tcttaaacgc attgctgacg ccgggcgatc tggtgttgtt
     1321 tgaccgcaat aaccataaat ctaaccacca cggtgcgttg ctacaggctg gcgcgacacc
     1381 ggtttatctg gaaaccgcac gtaacccgta tggttttatc ggtggtattg acgctcactg
     1441 ttttgaagaa agttacctgc gtgagctgat tacagaagtt gcaccgcagc gggcaaaaga
     1501 ggctcgtcct ttccgcctcg ccgttattca gctaggcact tacgacggta cgatttataa
     1561 cgcccgtcag gtggtggata aaattggtca tctgtgtgac tacatcctct ttgactccgc
     1621 atgggtcggc tatgaacagt ttattccgat gatggctgac tgttcgccgc tgttgctgga
     1681 tcttaatgag aacgatccgg gtattctggt tacgcaatcc gtacataaac agcaggctgg
     1741 tttttctcaa acttcacaaa tccataaaaa agacagccac atcaaagggc aacagcgtta
     1801 tgtaccgcac aaacgcatga acaacgcctt tatgatgcac gcctccacca gcccgttcta
     1861 tccgctgttt gccgcactgg atatcaacgc caaaatgcat gaaggtgtca gcggtcgtaa
     1921 tatgtggatg gattgtgtgg taaatggcat taatgcccgc aaactgatcc tcgataactg
     1981 tcagcatatt cgtccgttcg tacctgaact ggtggatggt aaaccctggc agtcgtatga
     2041 aacagcgcaa attgcggttg atctgcgctt cttccagttt gtaccagggg aacactggca
     2101 ttcttttgaa ggctatgcag agaatcaata ctttgtcgat ccttgcaaac tgttgctgac
     2161 aaccccaggt attgatgcac gtaacggcga atatgaagcg ttcggtgtac ccgcgacgat
     2221 tcttgctaac ttcctgcgcg aaaatggcgt agtgccggaa aaatgcgatc ttaactccat
     2281 cctcttcctg ctgactccgg cagaagatat ggccaagctt cagcaacttg ttgccctgct
     2341 ggtacgcttc gaaaaactgc ttgagtccga cgcgccatta gcagaagtgc tgccttccat
     2401 ctacaaacag catgaagagc gctacgccgg ttataccctg cgtcagttgt gtcaagaaat
     2461 gcatgatttg tatgcccgtc acaacgtgaa acaactgcaa aaagagatgt tccgtaagga
     2521 acacttccca cgcgtaagca tgaatccgca agaagccaac tacgcctatt tacgcggtga
     2581 agtggagctg gttcgtctgc cggatgcaga aggccgtatc gctgccgaag gtgcgcttcc
     2641 ttatcctccg ggtgtgctgt gcgttgttcc gggtgacatc tggggtggtg ctgttctgcg
     2701 ttacttcagc gctctggaag aagggatcaa cctgttgcca ggttttgcac cggagcttca
     2761 gggtgtctat atcgaagaac atgatggtcg taagcaagtt tggtgctatg tcatcaagcc
     2821 tcgtgatgcg caaagcaccc tgttgaaagg ggaaaaatta tgagtcaggc taaatcgaac
     2881 aaaatgggcg tcgttcagtt aaccatactg acgatggtca acatgatggg ctccggtatc
     2941 atcatgctgc cgacaaagct tgccgaagtt gggacaattt caattatctc ctggctggtg
     3001 acagccgtag gctcaatggc actggcatgg gcattcgcaa aatgcggtat gttcagccgt
     3061 aaatcaggtg gtatgggcgg ttatgccgag tatgctttcg gtaagtccgg caattttatg
     3121 gcgaactaca cctacggtgt ctcgctgctg attgctaacg tcgcgattgc tatttcggcg
     3181 gttggttacg gcaccgaact gctcggcgcg agtttgtcgc cagtgcagat tggtcttgcg
     3241 accatcggag tgctgtggat ttgtaccgtg gctaactttg gtggtgcgcg cattaccggg
     3301 caaatcagta gcattactgt gtggggggtc attattccgg tggtaggcct gtgcattatc
     3361 ggctggttct ggtttagccc gacgctttac gttgattcct ggaatccgca tcatgcaccg
     3421 ttcttcagtg cagtaggttc ttccatcgct atgacgctgt gggcttttct cggtctggag
     3481 tctgcgtgtg cgaatactga tgtagtggaa aacccggaac gtaatgtgcc aatcgcggta
     3541 ctcggcggta cgttaggtgc ggcggtgatt tatatcgtct ccaccaacgt gattgcaggg
     3601 attgtgccta atatggagct ggcaaattca acggcaccat ttggcctggc cttcgcacag
     3661 atgttcacac cagaagtggg taaagtcatt atggcgctga tggtgatgtc ctgctgcggt
     3721 tcgctgcttg gctggcagtt caccattgcc caggtgttta aatcttcatc tgatgaaggc
     3781 tacttcccta aaattttctc ccgtgtaacc aaagtggatg caccggtgca gggaatgttg
     3841 accattgtga ttatccagag cgggttggcg ctgatgacga ttagcccgtc gctaaacagt
     3901 cagttcaacg tgctggttaa cctggccgtg gtgaccaata tcattccgta tattctgtca
     3961 atggcggcat tagtcattat tcagaaggtc gctaatgtgc ccccctcaaa agcgaaagtt
     4021 gcaaactttg ttgcttttgt tggcgcgatg tatagctttt atgcgctgta ctcatccggg
     4081 gaagaagcca tgctgtacgg ttccatcgtg accttccttg gttggacact gtatggtctg
     4141 gtatcaccac gctttgaact gaaaaataaa cacggttaaa aaagcaaagt aaaaaagggc
     4201 gatcttgcga ccgccctttt tttattaaat gtgtttacgc gtttttcaga acttcgctaa
     4261 caatctcaac cgcttctttc tcaatctgct tgcgatgttc ttcaccgagg aagctttcgc
     4321 agtagatctt atatgcgtct tccgtgcctg acggacgcgc ggcgaaccag ccgttgtcag
     4381 tcatcacttt cagaccgccg atagaagcac cattgcccgg tgccgccgtc aggcgcgcgg
     4441 tgatcgggtc gcctgccagg gtgctggcgc tcaccatttc cggagacagc ttagacagcg
     4501 ccgctttttg tgcggaagtc gcaactgcct gcaaacggtt gtagctcggc gcaccaaagc
     4561 gttttgccag ttcgttgtag tgttcctgcg ggttcttacc ggtgaccgcg gtgatttccg
     4621 ccgccagcag acacatgatg atgccgtctt tgtcggtgga ccacggcgtg ccgtcgaaac
     4681 gcaggaagga agcccctgcg ctctcttcgc cgccaaaacc gaagctgccg tcgaacagac
     4741 catcaacaaa ccatttgaaa cctaccggta cttctaccag cttacggccc aagtcattga
     4801 ccacacggtc gatcatcgca gaagaaacca gcgttttacc gacagcaaca tctttgcccc
     4861 actgcggacg atgctggaac aggtagttga tcgccaccgc caggtagtgg ttcggattca
     4921 tcaaacctgc cggagtgacg ataccgtgac ggtcataatc cgggtcgtta gcaaacgcca
     4981 gatcgaactt atcacgcagt gccagcaggc ccgccatcgc acactcggag gagcagtcca
     5041 tacggatcgc gccgtcttta tcaaggtgca taaagcggaa ggtttgatcg acctgatcgt
     5101 taacgatggt caggttgagg ttgtaatact cgccaatacg cttccagtat tcgataccgg
     5161 aaccgcccag cggatcaacg cccagcgtca ggcccgcttt ctgaattgcc gccatatcaa
     5221 cgatatcggc cagaccttcc acaaacggct gcaccagatc ctgctctttt acatgaccgg
     5281 atgccatcgc ttcgtcgagg gagatacgct tcacgccttt caggccatcg gccatcagtg
     5341 cgttggccct gtcttccacc actttggtga cgttagtatc agccgggcca ccatttggcg
     5401 gattgtactt gataccacca tcttccggcg ggttatggga cggtgtaatc acgataccgt
     5461 ctgccagcgg gccacctttt ttattgtgaa gcaggatggc attggaaacg gcaggcgtcg
     5521 gagtgaagcc attgttttcc tgcacaatga catcaacgcc gttcgctgcc agcacttcca
     5581 gtacggaaat gaacgcaggc tcggacaggg cgtgagtatc tttacccaca tagcaagggc
     5641 cagtgatgcc atttttcgca cgttcttcag caattgcctg agcgatcgcc agaatgtgcg
     5701 gctcgttaaa gctgtggcgc gctgcactgc cacggtgacc ggaagtaccg aatttcaccg
     5761 cgtgctccgc attccctgct tctggtttca gtacataata ttgcgccgtc agttgggcga
     5821 cgttaatcaa atcactctgt tgtgcaggtt ggcctgcacg attgtggatt gccattgctt
     5881 tgtcctttgt ctgcaacgtt ttagatagtt ccgcgaacct tctcaatcaa ttccgccggg
     5941 aattgcatcg actgcatgat gtgttcgatc atgctgcatt tacggccggt gttggtgttg
     6001 gtgatcaccc aatacggcgt gcctggcaca tgtttcggct tggtctgatt accatttttc
     6061 agcagcgttt gttcatctgc cgcaaagtaa acgcgtgtac gaccgtgcaa cgattccgtt
     6121 gcttcggcaa acgcctgggc gtcaagagaa tatagtgtag acaacagcag cataaagcga
     6181 ttgaccgctc gcttttgctc tgcgtattcg tccgaaagca gaagttcacg cattgcgcga
     6241 accttgtctt taatcgtttt gaccggcttc gcttcgacga tagcaggtga cgcaacgcga
     6301 acctctttcg tcaccggagc agcaggctgt gatgaggcgg aaaatttcaa catacgccgt
     6361 aaaatgtcgg atgcgctctc gccgatatgc ttagtgtggc tggcaatata gctgtagagt
     6421 tcatcatcaa cttcaatcgt tttcatctta atccagtgca gtgtcttagc tgaatacaag
     6481 tcgccaggag tatatgaata aatcccggca gcggatagcg tcaagcctgg cttgcagaaa
     6541 aactcggaat aaaccttatt tgttgcagcc ctgtggcaca atggtcaaca taatacccta
     6601 acccgacaac gcgaaaaaaa gaactttgcc atgaaattga atatccgcgc gcaaactgca
     6661 caaaaccagc acaataattc tcccatcgtt cttgtccatg gtctgtttgg tagcctcgac
     6721 aaccttggcg tactggctcg cgatctggta aacgatcaca atatcatcca ggttgatatg
     6781 cgtaaccacg gtctttcacc gagagatccg gtaatgaatt acccggcgat ggcgcaggat
     6841 cttgttgata ctctggatgc acagcagatc gacaaagcaa catttatcgg tcactccatg
     6901 ggcggtaaag cggtaatggc acttactgca ctggcccccg atcgcatcga taaactggtg
     6961 gcgatcgata tcgcaccggt cgactatcac gtacgccgtc atgatgagat tttcgcggct
     7021 atcaacgcgg tcagtgaatc ggacgcacaa actcgccagc aagcagcagc aataatgcgc
     7081 cagcatctta atgaagaagg ggtgattcag tttctgttga aatcttttgt tgacggggag
     7141 tggcgcttta acgtgccggt attgtgggat cagtatccgc atattgtggg ttgggagaaa
     7201 atcccggcat gggatcaccc tgccctgttt atccctggcg gcaattctcc gtatgttagc
     7261 gagcagtacc gtgatgattt actggcacaa tttccacagg cacgggcgca tgtgattgca
     7321 ggcgcgggtc actgggtcca tgctgaaaaa ccggatgcgg tattacgcgc tatccgtcgc
     7381 tatctcaatg attaacgcaa ctgattaaaa acccagcgtc cgcgtcgctt tccgcgcgtg
     7441 ggcgttggcg tgactcatcg cgctgatgta ttatggcgcg ctatccatcc gggcagaggc
     7501 ctggctgatt gtttcccccg aagtcaccaa gatcatggcc aaagaacaaa cggaccgtac
     7561 gacattagat ctgttcgcgc acgagcgtcg accgggacga ccgaaaacta atccgctttc
     7621 gcgcgatgaa cagctgcgta ttaataaacg caaccagcta aaacgcgaca aagtacgtgg
     7681 ccttaagcgt gtcgaactga agctgaacgc ggaagctgtc gaggcgctga acgagctggc
     7741 ggagtcgcgc aatatgagcc gtagcgaact gatcgaagag atgttaatgc agcaactggc
     7801 ggcgctgcgt agtcagggca tcgtttaaat ttccactttc atgtagcaca gtgtgcagtc
     7861 ctgctcgttt gctgattccg caagactgcc tgttctgcta tgattgcctt tatccgtggg
     7921 caattttcca cccccatttc aataagtttc aagaggttat ttcactcatg gctatcactg
     7981 gcatcttttt cggcagcgac accggtaata ccgaaaatat cgcaaaaatg attcaaaaac
     8041 agcttggtaa agacgttgcc gatgtccatg acattgcaaa aagcagcaaa gaagatctgg
     8101 aagcttatga cattctgctg ctgggcatcc caacctggta ttacggcgaa gcgcagtgtg
     8161 actgggatga cttcttcccg actctcgaag agattgattt caacggcaaa ctggttgcgc
     8221 tgtttggttg tggtgaccag gaagattacg ccgaatattt ctgcgacgca ttgggcacca
     8281 tccgcgacat cattgaaccg cgcggtgcaa ccatcgttgg tcactggcca actgcgggct
     8341 atcatttcga agcatcaaaa ggtctggcag atgacgacca ctttgtcggt ctggctatcg
     8401 acgaagaccg tcagccggaa ctgaccgctg aacgtgtaga aaaatgggtt aaacagattt
     8461 ctgaagagtt gcatctcgac gaaattctta atgcctgatg tgatgcggcg tagactcatg
     8521 tctacgccgt attaatagat aatgccaatc aaaataattg ctacaaattt gtaacttttg
     8581 ctgttgtacc tgtacaatgt cccggtgttc aagtggcctt gccgttgtaa atgtaagctg
     8641 tgccacgttt ttattaacaa tatttgccag ggacttgtgg ttttcattta ggcgtggcaa
     8701 ttctataatg atacgcatta tctcaagagc aaattctgtc acttcttcta atgaagtgaa
     8761 ccgcttagta acaggacaga ttccgcatga ctgataacaa taccgcccta aagaaagctg
     8821 gcctgaaagt aacgcttcca cgtttaaaaa tcctggaagt tcttcaggag ccggacaacc
     8881 atcacgtcag tgcggaagat ttatacaaac gtctgatcga tatgggtgaa gaaattggtc
     8941 tggctacggt atatcgcgta ctgaaccagt ttgacgacgc tggtatcgtc acccgccaca
     9001 attttgaagg cggtaaatcc gtatttgaac tgacacagca acatcaccac gatcacctga
     9061 tttgcctcga ctgcggcaag gttatcgaat ttagtgatga ttccatcgaa gcgcgtcagc
     9121 gtgaaattgc cgcaaaacat ggcattcgcc tgactaacca cagtctctat ctttacggtc
     9181 actgtgccga aggcgattgc cgcgaagatg agcacgcgca cgaaggcaaa taagccagcc
     9241 tgaacgagaa aagccaacct gcgggttggc ttttttatac aagggaaata agaaaagcga
     9301 gagttacagc tctcacttat ttgttattac tgcgtatttc ctgccagacc ttatcacaat
     9361 cggctttcac cgcctgatca tttccggttt gcgttgcacg cagacactgc tgataatcca
     9421 gtacgcgtac actttcctgg tcggcaaact gctgatgttg tttctctttc ttcagcacgt
     9481 ttaacacgct ctggcaggct tcaatttttt ctggcgaacc ttgtgcggtg ttgatacagg
     9541 cgctgtatgc ctctttcaga cgactgtctt cctgtggcgc ggttgattgc gcacaagcta
     9601 ccagccccga tgccattatg gcgatgagaa tcagtttttt catagcaaat tcctcaaacg
     9661 tgcggcaggc acaaaacctg ccgcgtcggg catcagaaga tggtgaatgg tgcgattacc
     9721 ataaatttca cgtcacgctc atcctggaag atgttaccgt aaccgccgcc ccagcttggg
     9781 atgtcagagt ggttgtcgta ttcggtgaag tgcagtttga acatcgtgcc tttggcgcga
     9841 ccgtcctgaa tggtgtagac cgcatccagg ctgtaggcag actcttcaat agtccggttt
     9901 ttgtcgtagt acgcatccgg attgctctgc caggtcgcag gtttagcatc ccatgcgtaa
     9961 acgtaggaag cgccgatggc gaagcctgga agattccagt ttttcaggtc atacatcgca
    10021 ccgaagaaga ccgctttttc gccgttggcg ttgaagtcgg aacggttatc ccaccagata
    10081 tccaggcggc cgttggagga agcgtaggtt ggagtcatac gttgcaggaa gtatccctgc
    10141 tgaccgtcag ccttaaccca ggtgccttcg aggcgcaaat ccactacgtc agccgcccgg
    10201 taaccaaagg tcaacgcttg cagccaggcg gtgccgtcat aaaggtcgtt gacgctgcga
    10261 tcgtcaactt tatcgcgcgt accatagaac tggtagctgg tggttaacgg gctaccggcg
    10321 atatcaaatt tgtagctggc tttggcaaaa tattgatcga tatacccttc cgcctgacca
    10381 aatgccgctt ccagtacgaa gttatttttg aagtcgtatt tcgccccaag ggagtgcaga
    10441 taatcaactt tggtggtttt atcgttctga taaaactcat ccatttccag atgccacggc
    10501 gctttgtatt cgttggtcca catgtaggag aaactcaagg caccagcatc gccgtaatca
    10561 aaattcgccc cggcttccgc cccctgataa gtacctggca taaagctcca gtgcggcgct
    10621 aacagcgttt gaccagttgg ctgaatgtaa cctgccctcg cccaaaccgg accgtattta
    10681 aatttggccg cagctttata caggcttata ccgcttttgt cgccggacca gtcttcgtca
    10741 taggctttat tactttttga aaacgcgatt tcgttcgggt gggagctgtc gccgttttcc
    10801 gccatttcaa tcgccgtaaa cgcagcaata tcaaggccga acatatcagc agcataaccg
    10861 gactgaaaat cgaggttggc attccaggtg gagtgagaaa ggttggtttt gtatttgtcg
    10921 ccgtcggtaa catctttacg gtcgcgttca cgctgccagt aatatatacc gccggttaag
    10981 gttgaatcgt cgatgaatcc ttcggcgcga gcctccggca ttgccataaa gcccgacatt
    11041 gctgtaacac cggcgatagc cagcgccagc gtactacgtt tgccactaaa cgtacgcatg
    11101 ggttaatcct ctttgacgta taaattgctg caccaaaggt gaacagcgaa aaaataaaaa
    11161 ttaaaaaagt agcgttgaga aaagtctcgc tgactacagg aacagactat ttttcgcgac
    11221 gcgaattatc aatctctttc tgtaaccaga atgtaagaac atgagcaacc gcacatattt
    11281 tgatgatttt tgttacaagg cttaa
//
LOCUS       DAZGXZ010000155        11107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000155 DAZGXZ010000000
VERSION     DAZGXZ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.155"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            278..1492
                     /gene="manD"
                     /locus_tag="ACHKJF_004440"
     CDS             278..1492
                     /gene="manD"
                     /locus_tag="ACHKJF_004440"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFP0951690.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1559..2842
                     /locus_tag="ACHKJF_004441"
     CDS             1559..2842
                     /locus_tag="ACHKJF_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951691.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3094..4011
                     /gene="oxyR"
                     /locus_tag="ACHKJF_004442"
     CDS             3094..4011
                     /gene="oxyR"
                     /locus_tag="ACHKJF_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP0951692.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3994..5394)
                     /gene="sthA"
                     /locus_tag="ACHKJF_004443"
     CDS             complement(3994..5394)
                     /gene="sthA"
                     /locus_tag="ACHKJF_004443"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP0951693.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQTPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5725..6375
                     /gene="fabR"
                     /locus_tag="ACHKJF_004444"
     CDS             5725..6375
                     /gene="fabR"
                     /locus_tag="ACHKJF_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP0951694.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDIGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6375..6734
                     /gene="yijD"
                     /locus_tag="ACHKJF_004445"
     CDS             6375..6734
                     /gene="yijD"
                     /locus_tag="ACHKJF_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP0951695.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6774..7874)
                     /gene="trmA"
                     /locus_tag="ACHKJF_004446"
     CDS             complement(6774..7874)
                     /gene="trmA"
                     /locus_tag="ACHKJF_004446"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP0951696.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7988..8178
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8243..10087
                     /gene="btuB"
                     /locus_tag="ACHKJF_004447"
                     /pseudo
     CDS             8243..10087
                     /gene="btuB"
                     /locus_tag="ACHKJF_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     gene            10032..10889
                     /gene="murI"
                     /locus_tag="ACHKJF_004448"
     CDS             10032..10889
                     /gene="murI"
                     /locus_tag="ACHKJF_004448"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP0951697.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2569 a         2617 c         2958 g         2963 t
ORIGIN      
        1 catcaatcaa tacatatttt aattaattga atataattca tttctattgt tgtgcttatt
       61 tttaatgatt aatggagttt gttatgtgat tatttaacgt gttgttttaa cgatggtggt
      121 taattattca ggtggttttt gagggtattt tttctcctcg gcggaaattt ttatttttaa
      181 tgtgatcacg gttgaataat cgctctttct ctcttgtatg gtagtaggcg taaaattaag
      241 tttgaagcgt ctatttacaa cacaggagat tgcaataatg aaaattattg ctgcagatgt
      301 ttttgttact tgcccagggc gtaatttcgt tacgcttaaa ataaccactg aaagcggact
      361 ttgtggatta ggagatgcca cgttaaatgg tcgcgaattg tcggtcgctt cttatctgaa
      421 agatcatctt tgtccacaac taattggccg cgatgccagc cgtatcgaag atatctggca
      481 attcttttat aaaggtgctt actggcgacg tggccccgtc acgatgtcag ccatttctgc
      541 cattgatatg gcgttgtggg atatcaaagc taaagcggca aatatgccat tgtatcaact
      601 gcttggtggt gcttctcgtg aaggcgtgat ggtttattgc cacaccaccg ggcggacgat
      661 tgatgaagtc cttgaagact atgcaaagca ccagcagatg ggctttaagg cgattcgtgt
      721 gcagtgcggt gtaccgggta tgcaaactac ttatggcctg gcgaagggca aaggcctggc
      781 gtatgaaccg gcaaccaaag gcctgtggcc agaagagcag ttgtggtctt cagaaaagta
      841 tctcgatttt acgccgaagt tatttgaagc ggtgcgtaat aagttcggtt ttaacgaaca
      901 tctgttgcat gacatgcacc atcgcctgac ccccatcgaa gcggcgcgct ttgggaagag
      961 cattgaggat taccgcctgt tctggatgga agatccgacg cctgcagaga accaggaatg
     1021 ttttcgtctg attcgccagc ataccgtgac gccgattgcg gtcggtgaag tgtttaacag
     1081 tatctgggac tgcaagcagt taatcgaaga gcagctgatt gactatattc gcacgacgat
     1141 cacccatgca ggcggcatta ccgggatgcg gcggattgcc gattttgcat cgctttatca
     1201 ggtacgtacc ggttcgcatg gcccttcaga tttatcgcct gtttgtcatg cggcggcgct
     1261 gcattttgac ctatgggtac caaattttgg cgttcaggag tacatgggct attccgaaca
     1321 aatgcttgaa gttttcccgc acagctggcg cttcgaggaa ggttatatgc acccaggcga
     1381 tgaaccgggg ctgggtattt ctttcgatga gaaactggcg gcgaaatatc cgtatgaccc
     1441 ggcgtatctc cccgtcgcac gactggaaga tggcacattg tggaactggt aataacggtt
     1501 cgcttaatta ttttctgaat agtcatcaaa tcgattaatt ccgctcaagg agctaaaaat
     1561 ggacgttgat gtgtcaacca gtgttgcggg taataaaccg caacggattc gtcgtattca
     1621 gacagtgacc ctggttttat tatttatggc ggggatcgtt aattttctcg accgttcgtc
     1681 attgagcgtg gcaggtgaag cgattcgtgg cgagttagga ttatcggcga cagagtttgg
     1741 tgttttgctt tctgcatttt ctctgtctta tggtttttca caactacctt ccggtatttt
     1801 gctagaccgc tttggcccac gaattgtttt aggcgcaggc ttaatattct ggtcattaat
     1861 gcaggcatta acaggaatgg ttaattcttt tagccacttt attttaatgc gtatcggtct
     1921 gggtattggc gaagcgccat ttatgcctgc gggagtaaag tcgatcaccg actggtatgc
     1981 acaaaaagaa cgcggcacgg cgctggggat ttttaactca tctaccgtta tcggtcaggc
     2041 tatcgcgcct cctgctcttg tattgatgca gctggcatgg ggctggcgga cgatgttcgt
     2101 tatcatcggc gtggcaggga ttctggttgg gatctgttgg tacgcgtggt atcgcaaccg
     2161 ggcgcagttt gtcctgactg acgaagaacg gacgtatctc tccgcgccgg ttaaaccgcg
     2221 cccacagctg caatttagcg agtggctggc gctgtttaag catcggacaa cctgggggat
     2281 gattttaggt ttctctggcg tcaattatac cggttggctc tacatcgcgt ggttacccgg
     2341 ttatttgcaa gccgagcaag gtttcagtct ggcgaaaacc ggttgggtgg cggcgattcc
     2401 tttcctcgcg gcggcagtcg ggatgtgggt taacggtatt gttgtcgatc gactggcgaa
     2461 aaaaggctac gacctggcga agacgcgtaa aacggctatt gtctgcggtt tgatgatgtc
     2521 ggcattaggc acgttgctgg tcgtgcaatc ttcctcgcca gcccaggcgg tagcgtttat
     2581 ctcaatggcg ctattctgcg tgcatttcgc tggaacgtct gcatgggggc tggtgcaggt
     2641 gatggtgtca gaaacaaaag tggcttccat cgccgggatt caaaactttg gcagttttgt
     2701 ttttgcttcc tttgctccga tcgtaaccgg ttgggtagtg gataccacac actcgtttaa
     2761 tctggcgttg gttatcgcgg cctgtgtgac gttcaccggg gcgctgtgtt acttctttat
     2821 tgtcaaagat cgcattgagt aatcgttact atcaggctct cttgctgtgg gcctgtagaa
     2881 taaaaaaaag cggatcctgg agatccgcaa aagttcacgt tggctttagt tattcgagtt
     2941 gagaaactct cgaaacgggc agtgacttca agggttaaaa gaggtgccgc tccgtttctg
     3001 tgagcaatta tcagtcagaa tgcttgatag ggataatcgt tcattgctat tctacctatc
     3061 gccatgaact atcgtggcga tggaggatgg ataatgaata ttcgtgatct tgagtacctg
     3121 gtggcattgg ccgagcaccg ccattttcgg cgtgcggcag attcctgcca cgttagccag
     3181 ccgacgctta gcgggcaaat tcgtaagctg gaagatgagt tgggcgtgat gttgctggag
     3241 cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc tgctggtaga tcaggcgcgt
     3301 accgtactgc gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg cgagacgatg
     3361 tccgggccgc tgcacattgg tttgattccc acagttggac cgtacctgct accgcatatt
     3421 atccctatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc
     3481 caccagttac tggcgcaact ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg
     3541 aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt gctggctatc
     3601 tatgaagatc acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa
     3661 aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt
     3721 gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggagac gctgcgcaac
     3781 atggtggcgg caggtagcgg gatcacttta ctgccagcgc tggctgtgcc gccggagcgc
     3841 aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc
     3901 ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc
     3961 cgagcaagaa tggatggcca tttcgataaa gtgttaaaac aggcggttta aaccgtttaa
     4021 cgcagctacc cgataggctt ccgccatggt cgggtagtta aaggtggtgt tgacgaagta
     4081 ctcaatagtg ttgccgccac ctttctgttc cataatcgcc tgaccgatat gaataatttc
     4141 ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct tttgtttccc gatggaacaa
     4201 aattttcagc gtgcccacgt tcatgccgac gatttgtgcg cgtgccagat gtttaaactg
     4261 ggcgcggccc acttcatatg gcactttcat cgcggtcagc tgctgttcgg ttttgcccac
     4321 agagctgatt tccgggatgg tgtaaatgcc ggtagggata tcttcaatca gatgcgcggt
     4381 ggcttcgcct tttaccagcg cctgcgcggc aatgcgcccc tggtcatagg ccgctgatgc
     4441 caggctcgga taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata
     4501 catgctgttg accttcagct gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc
     4561 cagcgaatcg gtattaccgg tacgaccgtt ggcatagagc aggcagtcag ctttcagttt
     4621 tttacccgac ttcagatgca tgatcacacc gtcgtcacag ccttcgatct tctcgtactc
     4681 ttcgttgtga cgaatcacta cgccactgtt ccagaagtga taggagagag aatctgacat
     4741 ctcttgatcg agaaatgcca gcaggcgatc gcgggtgttg atcagatcca cttttacatc
     4801 cataccgcgg aagatcgacg catattcaca gccgatcact ccagcaccat agataagtac
     4861 atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg tcgtaaatgc gtggatgggt
     4921 gaaatcaaca tctgttggat gatatggacg agagccgcag gcaataacaa atttttcagc
     4981 ggttagtgtt tcaacgctgc cgtccgggca atccagcgcc aacgtatgct cgtcaacaaa
     5041 gcgagcgttt ccctgcaata tttcacagtg attacgttcg taaaatccct gacgcatgcg
     5101 cgtttgttga ttaatcacgt tatcggcatg gttaaggata tcggcaaaag aagagcggag
     5161 cagtcgggaa tggtcgctgt aaagtggggt ttgattgaat tctataatgc ggctgacggc
     5221 gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc caacattttg
     5281 ataacgctcg ataactgcga cgcgcgcacc ttgcttaacc aggcccattg cagcgccttc
     5341 gccgccgggg ccggaaccta ttactatggc atcgtaatcg taggaatgtg gcatggtagg
     5401 gcttacctgt tcttatacat aaaagcaaca gaatggtaac attttatcgc gggtaagcca
     5461 attgatcccc gtcatttatc tggctatatc ctgagcggcc tttgctttgt ctgtttctta
     5521 cttttgccct gacgttttat tggattttta tcgacgatac tctccgttta agcggcaggt
     5581 ttccgctgta cgtaaaagaa ccggccaaag aattgcagta aatatgtttt attgcgttac
     5641 cgttcattca caatactgga gcaatccagt atgttcattc tctggtatag tgccagcagt
     5701 acttttggca aggattcaga catcgtgatg ggcgtaagag cgcaacaaaa agaaaaaacc
     5761 cgccgttcgc tggtggaagc cgcatttagc caattaagtg ctgaacgcag cttcgccagc
     5821 ctgagtttgc gtgaagtggc gcgtgaagcg ggcattgctc ccacctcttt ttatcggcat
     5881 ttccgcgacg tagacgaact gggtctgacc atggttgatg agagcggttt aatgctacgc
     5941 caactcatgc gccaggcgcg tcagcgtatc gccaaaggcg ggagtgtgat ccgcacctcg
     6001 gtctccacat ttatggagtt catcggtaat aatcctaacg ccttccggtt attattgcgg
     6061 gaacgctccg gcacctccgc tgcgtttcgt gccgccgttg cgcgtgaaat tcagcacttc
     6121 attgcggaac ttgcggacta tctggaactc gaaaaccata tgccgcgtgc gtttactgaa
     6181 gcgcaagccg aagcaatggt gacaattgtc ttcagtgcgg gtgccgaggc gttggacatc
     6241 ggcgtcgaac aacgtcggca attagaagag cgactggtac tgcaactgcg aatgatttcg
     6301 aaaggggctt attactggta tcgccgtgaa caagagaaaa ccgcaattat tccgggaaat
     6361 gtgaaggacg agtaatgaaa caagcaaatc aagatagagg tacgctgctg ctggcgttag
     6421 ttgctggctt atcgattaat ggtactttcg cagcgctgtt tagctccatt gtgccatttt
     6481 ctgtattccc gattatttcc ctggtgctga cggtttactg cctgcatcaa cgttatctta
     6541 atcgcactat gccggtaggc ttgccgggtc tggcagctgc ctgttttatt ctcggcgtac
     6601 tgctgtacag cacggtagtt cgtgcggaat atccggatat cggctctaac ttcttcccgg
     6661 cagtactctc cgtcattatg gtgttctgga ttggcgcgaa gatgcgtaac cgtaagcagg
     6721 aagttgctga gtaatcggaa tcatgccgga tgctggcgca tccggcatgg gttttacttc
     6781 gcggtcagta atacgccgca ctccatatgg tgcgtgtagg ggaactgatc aaacagagcc
     6841 agacgttcga ccttgtgcgt ctggcttaat gtttccagat tcttgcataa cgtttccgga
     6901 ttgcaggaga tatacagaat acgtggatac gcctgcacca ttttctcggt ttcactgtcc
     6961 agaccgctgc gcggcgggtc gacaaaaatg gtttcgcact gataactctt caagtcgatc
     7021 ccttgcaggc gattaaactc gcgtacacca ttcatcgcct gagtaaattc ttctgccgcc
     7081 atacgaataa tttgtacgtt atcaatatgg ttagctgcga tgttgtattg cgcagcagca
     7141 accgacggct tagcgatttc ggtggctaat acccgatcaa aattgcgcgc cagtgctaat
     7201 gaaaagttac cgttgccgca gtacagctcc agtaaatcgc cttttgagcc tttggttacg
     7261 tccagcgccc attccagcat ctgaatattc atcgccgcgt tcggctgggt aaaactgttt
     7321 tctacctgac ggtagatcat ctctttccct gcgaccggca gacgttcatc gatgtaatct
     7381 tgatccagct cgattttggt tttcgttgcc cgaccaatca gatgcacatt cagattctgc
     7441 gcgcgcagtg catcgcgcag ggcttccgct tcctgacgcc actcatcatc cagcttttta
     7501 tggtacaaca gggaaaccac cgcctgatta ctcagcgtgg tgaggtaatc aatctggaac
     7561 aacttgtggc gcagaacggg attattacgc acacccgcaa tcatcgccgt catcaactgg
     7621 ttgatgagtt cactggcagc ggggaagcta tccacgcgga tgcggctttt ggtttgttga
     7681 tcgaaaatga tgtgatacag gtcatcgcca tcgtgccaga tgcggaactc cgcacgcatc
     7741 cggtaatgac tgaccggcga gcgaaacact tccggaacca ggtcagaaaa cggtgccatc
     7801 atactttgca aacgtaccac tttttcggct aactgcgctt catactgttc tgttggaagg
     7861 tgttcggggg tcatgatgta tcctgaaaaa ttaaagtacg cggcgattgt agggattgct
     7921 catcagatgt ccagatcttg atgaattcct atttgtgagc tacgtctgga cagtaacttg
     7981 ttacaacctg tagcatccac ttgccggtcc tgtgagttaa tagggaatcc agtgcgaatc
     8041 tggagctgac gcgcagcggt aaggaaaggt gcgatgattg cgttatgcgg acactgccat
     8101 ccggtgggaa gtcatcatct cttagtatct tagatacccc tccaagcccg aagacctgcc
     8161 ggccaacgtc gcatctggtt ctcatcatcg cgtaatattg atgaaacctg cggcatcctt
     8221 cttctattgt ggatgcttta caatgattaa aaaagcttcg ctgctgacgg cgtgttctgt
     8281 cacagccttt tccgcttggg cacaggatac cagcccggat actctcgtcg ttactgctaa
     8341 ccgttttgaa cagccgcgca gcactgtgct tgcaccaacc accgttgtga cccgtcagga
     8401 tatcgaccgc tggcagtcga cctcggtcaa tgatgtgctg cgccgtcttc cgggcgtcga
     8461 tatcacccaa aacggcggtt caggtcagct ctcatctatt tttattcgcg gtacaaatgc
     8521 cagtcatgtg ttggtgttaa ttgatggcgt acgcctgaat ctggcggggg tgagtggttc
     8581 tgccgacctt agccagttcc ctattgcgct tgtccagcgt gttgaatata tccgtgggcc
     8641 gcgctccgct gtttatggtt ccgatgcaat aggcggggtg gtgaatatca tcacgacgcg
     8701 cgatgaaccc ggaacggaaa tttcagcagg gtggggaagc aatagttatc agaactatga
     8761 tgtctctacg cagcaacaac tgggggataa gacacgggta acgctgttgg gcgattatgc
     8821 ccatactcat ggttatgatg ttgttgccta tggtaatacc ggaacgcaag cgcagacaga
     8881 taacgatggt tttttaagta aaacgcttta tggcgcgctt gagcataact ttactgatgc
     8941 ctggagcggc tttgtgcgcg gctatggcta tgataaccgt accaattatg acgcgtatta
     9001 ttctcccggt tcaccgttgc tcgatacccg taaactctat agccaaagtt gggacgccgg
     9061 gctgcgctat aacggcgaac tgattaaatc acaactcatt accagctata gccatagcaa
     9121 agattacaac tacgatcccc attttggtcg ttatgattcg tcggcgacgc tcgatgagat
     9181 gaagcaatac accgtccagt gggcaaacaa tgtcatcgtt ggtcacggta gtattggtgc
     9241 gggtgtcgac tggcagaaac agactacgac gccgggtaca ggttatgttg agaatggata
     9301 tgatcaacgt aataccggca tctatctgac cgggctgtaa caagtcggcg attttacctt
     9361 tgaaggcgca gcacgcagtg acgataactc acagtttggt cgtcatggaa cctggcaaac
     9421 cagcgccggt tgggaattca tcgaaggtta tcgcttcatt gcttcctacg ggacatctta
     9481 taaggcacca aatctggggc aactgtatgg cttctacgga aatccgaatc tggacccgga
     9541 gaaaagcaaa cagtgggaag gcgcgtttga aggcttagcc gctggggtga actggcgtat
     9601 ttccggatat cgtaacgatg tcagtgactt gatcgattat gatgatcaca ccctgaaata
     9661 ttacaacgaa gggaaagcgc ggattaaggg cgtcgaggcg accgccaatt ttgataccgg
     9721 accactgacg catactgtga gttatgatta tgtcgatgcg cgcaatgcga ttaccgacac
     9781 gccgttgtta cgccgtgcta aacagcaggt gaaataccag ctcgactggc agttgtatga
     9841 cttcgactgg ggtattactt atcagtattt aggcactcgc tatgataagg attactcatc
     9901 ttatccttat caaaccgtta aaatgggcgg tgtgagcttg tgggatcttg cggttgcgta
     9961 tccggtcacc tctcacctga cagttcgtgg taaaatagcc aacctgttcg acaaagatta
    10021 tgagacagtc tatggctacc aaactgcagg acgggaatac accttgtctg gcagctacac
    10081 cttctgaacc acgtcccacc gtgctggtgt ttgactccgg cgtcggtggg ttgtcggtct
    10141 atgacgagat ccggcatctc ttaccggatc tccattacat ttatgctttc gataacgtcg
    10201 cttttccgta tggcgaaaaa agcgaagagt ttattgttga gcgagtggtg gcaattgtca
    10261 ccgcagtgca agaacgttat ccccttgcgc tggctgtggt cgcttgcaac accgccagta
    10321 ccgtttcact tcctgcatta cgcgaaaagt tcgacttccc ggttgttggt gtcgtgccgg
    10381 cgattaaacc tgctgcacgt ctgacggcaa atggcattgt cggattactg gcaacccgcg
    10441 gaactgttaa acgttcttat actcatgagc tgatcgcgcg tttcgctaat gaatgccaga
    10501 tagaaatgct gggctcggca gagatggttg agttggctga agcgaagctg catggcgaag
    10561 atgtttctct ggatgcacta aaacgtatcc tacgcccgtg gttaagaatg aaagagccgc
    10621 cagataccgt tgtattgggt tgcacccatt tccctctact acaagaagaa ctcttacagg
    10681 tgctgccaga gggaacccgg ctggtggatt ctggcgcagc gattgctcgc agaacggcct
    10741 ggctgttaga acatgaagct ccggatgcaa aatctgccga tgcgaatatt gccttttgta
    10801 tggcaatgac gccagaagct gaacaattat tgcccgtttt acagcgttac ggcttcgaaa
    10861 cgctcgaaaa actggcagtt ttaggctgat ttggttgaat gttgcgcggt cagaaaatta
    10921 ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc actgacacgg
    10981 aacaacggca aacacgccgc cgggtcagcg gggttctcct gagaactccg acagagaaag
    11041 caaaaataaa tgcttgactc tgtagcggga aagcgtatta tgcacacccc gcgccgctga
    11101 gaaaaag
//
LOCUS       DAZGXZ010000156        11100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000156 DAZGXZ010000000
VERSION     DAZGXZ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.156"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            373..1356
                     /locus_tag="ACHKJF_004449"
     CDS             373..1356
                     /locus_tag="ACHKJF_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFP0951698.1"
                     /translation="MPEKYSPDDQSSTIDPYLITSVRQTLEEQGAALQNLSKQLDSGQ
                     YQCVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PCDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIHQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            1428..2576
                     /locus_tag="ACHKJF_004450"
     CDS             1428..2576
                     /locus_tag="ACHKJF_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFP0951699.1"
                     /translation="MLIKVKSAVSWMRAHLSAISLSDIQKHLVKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEKSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            2600..4276
                     /locus_tag="ACHKJF_004451"
     CDS             2600..4276
                     /locus_tag="ACHKJF_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFP0951700.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDISADPNLTGAAPLTGILNGQK
                     SDTQNMSGFDNTPPPAPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            4286..5026
                     /gene="kdsB"
                     /locus_tag="ACHKJF_004452"
     CDS             4286..5026
                     /gene="kdsB"
                     /locus_tag="ACHKJF_004452"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0951701.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEAAEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMPEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            5023..7050
                     /locus_tag="ACHKJF_004453"
     CDS             5023..7050
                     /locus_tag="ACHKJF_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFP0951702.1"
                     /translation="MIGIFSSGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLSVNGAPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNTALISKAREAMHTIVTGDLSKYNLAPAFVADESECSDIVLVVDQTFND
                     MSVTYGNAGQNEFSAMLEAAMAENPQATIWVKVHPDVLKGKKTGYFADLRATQRVRLI
                     TENISPQSLLRHISRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSVTLEELFAAAYLRYCRYIDPLTGAASDLFTVLQWLQLLRNHQQQRNGYLWAP
                     GLTLWKSAILKPFLRTATNRLSYSRRCTAATACVVWGVKGEQQWQVEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPSLSLVLDKRGIYYDATRPSDLEVLLNHSQLTLAQQMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKGKKVILVPGQVEDDSSIKTGTISIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAELIAELADYQALDADIIQCIQHADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTFDEHHCGRRERELTLADLLYQALIVYPTYI
                     HPTRLQPITVEEAAEYLIQTSRKPMFITRKKAGWVIRYYRKLIMFCKVRFG"
     gene            7085..8284
                     /locus_tag="ACHKJF_004454"
     CDS             7085..8284
                     /locus_tag="ACHKJF_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0951703.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFSDVAEWLESLGRNAVNVV
                     FNGGDCFYCRHRHYQAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKYWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPAPHVENLKPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRSYWRKQLYKVTQRKVLPR
                     LMNELDQLYYLAVLQVYNDSQIRNHSNYNDVRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGGRVIYVHDLPMPGLLRHAKAVVTINSTVGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYILMKTQLNAVYYGIF
                     NFRKITN"
     gene            9101..9877
                     /locus_tag="ACHKJF_004455"
     CDS             9101..9877
                     /locus_tag="ACHKJF_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0951704.1"
                     /translation="MKRSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPAAHLL
                     VLLGIFGYIMHRTMPGISFPVFLLNGLIPFFIFNSISNRSVEAIDANQGLFNYRPVKP
                     MDTIIARTLLETLIYIIVYVLLMLIVWMSGEYFEIISLLQLVLVWTLLIIISCSVGLI
                     FMVVGKTFPEMQKILPIFLKPLYFISCIMFPLHSIPQQYWTYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
     gene            9874..10545
                     /locus_tag="ACHKJF_004456"
     CDS             9874..10545
                     /locus_tag="ACHKJF_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0951705.1"
                     /translation="MIKIENLTKSYRTSTGRHYVFKDLNIELPSGKSVAFIGRNGAGK
                     STLLRMIGGIDRPDRGKIITNKTISWPIGLAGGFQGSLTGRENVKFVARLYAKPEELK
                     DKIEFVEEFAELGKYFDMPIKTYSSGMRSRLGFGLSMAFKFDYYIVDEVTAVGDARFK
                     EKCAHYFKERHKESSFLMVSHSLNSLKEYCDVVIYIKSFNEVKYYSVVTDALAEYIID
                     TKIQV"
     gene            10571..>11100
                     /locus_tag="ACHKJF_004457"
     CDS             10571..>11100
                     /locus_tag="ACHKJF_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 1"
                     /protein_id="HFP0951706.1"
                     /translation="MSIIVNHVMSSDIVSGIFTDLLSYYKRYCDRDISIVESSNAIDN
                     ADVYHYHRPHLESRLKENSVVTIHHDLYDTDPWLDYDKFHPRYMEAKKILCLNSSQQN
                     YLNNKGLMNTEIIPHGYNSDIFCSVGAQKKIKNKVCIGFISKRYGRKVKGEAYLYEIF
                     KRLDSKRYRFIFVGVD"
BASE COUNT         3039 a         2395 c         2705 g         2961 t
ORIGIN      
        1 tttaataagt tacaaccagt attcatttca ggaatgaatg tgggaatcga acccataatt
       61 ggcgtcaaag gctgacggaa cacgcccgga aaacagcctg gccgttcctg gtgtgccgac
      121 ggggtttgac cccctttgta attcagtttc aacgcgtacg agatattgcg agaagcgtgc
      181 cagaattgag aaaaaattat taaccaaatg aaaaataaag atttaataaa ttatttttgt
      241 ttgtagactg tgttatatgc tttatggaaa agtagtattt tgatttatgt gaagatgagc
      301 tgaactcgat ttatcagagg caagttgtct ttatgttata agtgttttaa ccttatcgga
      361 agtataattt atatgccaga aaaatattca cctgatgatc agagcagtac tattgatcca
      421 tatttaatta cttctgttcg tcagaccttg gaagaacaag gtgcagcatt acaaaacttg
      481 tctaaacaac tggattccgg gcagtaccag tgtgtcctta atttgataat gaactgtaaa
      541 gggcacgtta ttctttcggg aatgggtaaa tcagggcatg tcggtcgtaa aatgtcagcg
      601 acgctggcct ctaccggtac cccgagtttc tttattcatc ctgcagaagc gttccatggc
      661 gatctgggta tgattacgcc ctgcgatctt ttgatcctta tttctgccag cggtgaaacg
      721 gatgaaatcc tcaagctagt tccttcactg aaaaatttcg gcaaccgaat tatcgccatt
      781 accaataatg gaaattccac gctggcgaaa aatgctgatg ccgtgctgga actccatatg
      841 gcgaatgaaa cctgcccgaa taatcttgca ccaacaacgt ctaccaccct gaccatggcg
      901 atcggcgatg cgctggcgat tgccatgatc caccaacgca aatttatgcc gaatgatttt
      961 gcgcgctatc acccgggcgg ttcattaggt cgtcgcctgc tgacccgcgt tgctgatgtc
     1021 atgcagcatg atgttcctgc ggtacagctg gatgcgtcat ttaaaaccgt gattcaacgt
     1081 atcaccagcg gatgccaggg aatggtgatg gtagaagacg cagaaggtgg gctggcgggc
     1141 attataaccg acggtgacct gcgtcgcttt atggaaaaag aggattctct gacatccgct
     1201 acggctgcgc agatgatgac acgtgaaccg ctgacgctac cggaagacac catgatcatt
     1261 gaagcggaag aaaaaatgca aaagcaccgc gtctcaacgt tattggtgac caacaaggca
     1321 aataaagtca ctggccttgt gcgcattttc gactaattaa gcaacggggt ttgagaggtt
     1381 agtctctcat tgagtttgtg ccccactgga actttgataa tcgttacatg ttgataaaag
     1441 tgaagtctgc cgtatcctgg atgcgtgctc atctgtctgc catctcactg tcagatatcc
     1501 aaaaacacct ggtgaaaatc atcattctgg caccgatggc ggtgctgctg atctatctgg
     1561 ctatcttcag ccagcctcgc tatatgagcg agtcgaaagt cgccattaaa cgctcggatg
     1621 atttaaacag cggcagcctg aattttggtc tgcttctggg tgcctctaac cccagttccg
     1681 cagaagatgc gttgtatctg aaagagtaca tcaactcgcc ggatatgctg gcggcgctgg
     1741 ataaacaact aaattttcgt gaagcgttca gccacagcgg gctcgatttt cttaatcatc
     1801 ttagcaagga tgaaaccgca gaaggcttcc tgaagtacta caaggaccgt atcaacgtct
     1861 cgtatgacga taaaaccgga ttactgaata ttcagacgca gggctttagc ccggagtttg
     1921 cgcttaagtt taaccagacc gtgctgaaag agtcagagcg ctttatcaat gagatgtcac
     1981 atcgcatcgc gcgtgaccag cttgcctttg cagaaacgga gatggaaaag gcacgccagc
     2041 gtctggacgc cagcaaagcg gaattgctct cttatcagga caacaacaac gttctggatc
     2101 cacaggcaca ggcacaggcg gcgagcacgt tagtcaatac gctgatgggc cagaagatcc
     2161 agatggaagc ggatctgcgt aacttgctga cgtatctgcg tgaggacgcc ccgcaagtgg
     2221 tgagtgcgcg taatgcgatt cagtcattac aggcacaaat cgatgaagaa aaaagcaaaa
     2281 tcactgcacc acagggtgac aagctaaacc gtatggcggt ggattttgaa gaaatcaaat
     2341 caaaagttga gttcaacacc gagctctaca aactgaccct gacctccatt gaaaagaccc
     2401 gtgtagaagc agctcgtaag ctcaaggtgc tgtcagtgat cagttcgcca cagttgccgc
     2461 aggaatcgtc ttttccaaat atcccttatt tgatcgcctg ctggttactg gtgtgctgcc
     2521 tgctgttcgg caccctgaaa ctgttgctgg ctgtcattga agatcaccga gactaacgct
     2581 gtcgctgaat gagtttgtga tgaaattatt taaatcaatt ttactgattg ccgcctgtca
     2641 cgcggcgcag gccagcgcgg ccattgatat tagcgctgac ccaaacctga caggagccgc
     2701 gccgcttacc ggtattctga acggacagaa gtcggatacg caaaacatga gcggcttcga
     2761 caatacccca ccgcccgcac cgccggtggt aatgagccgt atgtttggtg ctcaactttt
     2821 caacggcacc agcgcggata gcggtgcgac ggtaggattc aaccctgact atattctgaa
     2881 tccgggtgat agcattcagg ttcgcttgtg gggtgcgttc acctttgatg gtgcgttaca
     2941 ggttgatccc aaaggtaata ttttcctgcc gaacgttggt ccggtgaaag ttgctggcgt
     3001 cagtaatagt cagctaaatg ccctggtcac atccaaagtg aaggaagtat accagtccaa
     3061 cgtcaacgtc tacgcctcct tattacaggc gcagccagta aaagtgtacg tgaccggatt
     3121 tgtgcgtaat cctggtctgt atggcggtgt gacgtctgat tcgttactca attatctgat
     3181 caaggctggc ggcgttgatc cagagcgcgg aagttacgtt gatattgtgg tcaagcgcgg
     3241 taaccgcgtg cgctccaacg tcaacctgta cgacttcctg ctgaacggca aactggggct
     3301 ttcgcagttc gccgatggtg acaccatcat cgtcgggccg cgtcagcata ctttcagcgt
     3361 tcagggcgat gtctttaaca gctacgactt tgagttccgc gaaagcagca ttcccgtaac
     3421 ggaagcttta agctgggcgc gccctaagcc tggcgcgact cacattacga ttatgcgtaa
     3481 acaggggctg caaaaacgca gcgaatacta tccgatcagt tctgcgccag gtcgtatgtt
     3541 gcaaaatggc gataccttaa tcgtgagcac tgaccgctat gccggtacca ttcaggttcg
     3601 ggttgaaggc gcgcactccg gtgaacatgc catggtactg ccttatggtt ccactatgcg
     3661 tgcggttctg gaaaaagtcc gcccgaacag catgtcgcag atgaacgcgg ttcagcttta
     3721 tcgcccatca gtggctcagc gtcagaaaga gatgctgaat ctctcgctgc aaaaactgga
     3781 ggaagcatca ctttctgccc agtcctccac caaagaagaa gccagcctgc gaatgcagga
     3841 agcacaactg atcagccgct ttgtggcgaa agcacgcacc gtggttccga aaggtgaagt
     3901 gatcctcaac gaatccaata ttgattctgt tctgcttgaa gatggcgacg tcatcaatat
     3961 tccggagaaa acatcgctgg ttatggttca tggcgaagtg ctgttcccga acgcggtgag
     4021 ctggcagaag ggtatgacca ccgaggatta catcgagaaa tgtggtggcc tgacgcaaaa
     4081 atcgggtaac gccagaatta tcgtcattcg tcagaacggt gctgccgtca acgcggaaga
     4141 cgtggattca ctcaagccgg gcgatgagat tatggttctg ccgaaatatg aatcgaaaaa
     4201 tattgaagtt acccgtggta tttccacaat cctctatcag ctggcggtgg gtgcaaaagt
     4261 gattctgtct ttgtaaggag ttgagatgag caaagcagtt attgtcatcc cggctcgcta
     4321 tggctcctcg cgcctgccgg gtaagccact gctcgatatt gttggtaaac cgatgatcca
     4381 gcatgtttac gagcgtgcgt tacaggtggc gggcgtcgca gaagtttggg tggcaacaga
     4441 cgatcagcgt gttgaaaagg ccgtgcaggc atttggcggg aaagccatca tgacgcgcaa
     4501 cgatcatgaa tccggcaccg atcggctggt cgaggtgatg cataaagtcg aggcagatat
     4561 ctacattaac ctgcagggcg acgaaccaat gattcgcccg cgggatgtag aaacgctgtt
     4621 acaaggaatg cgtgatgacc ccgcgttgcc agtggcaacg ctatgccacg cgatttctgc
     4681 cgaagaagcg gctgagccaa gcacggtaaa agtggtggta aatacccacc aggatgcgct
     4741 ttatttcagc cgttcgccga ttccgtatcc gcgtaatgct gaaaaagcgc gctacctgaa
     4801 acacgttggt atttacgctt atcgtcgcga tgtgctgcaa aactacagcc agttaccgga
     4861 gtccatgcca gagcaggcag aatcactgga gcagctgcgg ttaatgagcg cggggatcaa
     4921 catccgcaca tttgaggttg ccgcaaccgg tccgggcgtc gacaccccag catgcctgga
     4981 aaaagtgcgc gccctgatgg cacaggaact ggctgaaaac gcatgattgg cattttctcg
     5041 tccggtatct ggcgtattcc gcatctggag aaatttctgg cgcaaccgtg ccagaaactt
     5101 tctctgctgc gccctgttcc gcaagaagtt gatgctatcg ccgtgtgggg acatcgtccc
     5161 agcgcggcaa aaccggtcgc catcgccaaa gcagcgggaa aacccgtcat ccgtctggaa
     5221 gatggatttg tgcgttcgct ggatcttagc gtcaatggtg cgccgccgct ttctctcgtg
     5281 gtggatgatt gtggcattta ctacgatgcc agcaagcctt cagcactgga gaaactggta
     5341 caggataaag ccggaaatac agctctgata agtaaggcca gagaggcgat gcacaccatc
     5401 gtgaccgggg atttgtcgaa atataacctg gcgcctgcgt ttgtggctga tgagtcagaa
     5461 tgttcagaca tcgttctggt tgtcgatcag acatttaatg atatgtcagt gacgtatggt
     5521 aatgctggtc agaatgagtt ttctgccatg ctggaggccg cgatggcgga aaatcctcaa
     5581 gcgacaattt gggtgaaggt gcacccagat gtactgaaag gaaagaaaac aggttatttc
     5641 gccgatctgc gcgccacgca acgagtacgt ttgattacgg agaatatcag tccgcagtcg
     5701 ctgttgcgac atatttccag agtttacgtc gtgacctccc aatacggctt tgaggcctta
     5761 ctggcaggaa aaccagtgac atgcttcggt caaccctggt atgcaggctg gggcttaacg
     5821 gacgatcgtc atccgcagtc ggctttgtta tctgcccgac gcggttctgt cacgctggaa
     5881 gaactttttg ccgcagcata cctgcgttac tgtcgctata tcgacccact aacgggggca
     5941 gcaagtgacc tgtttaccgt actgcaatgg ctgcaattac tgcgcaacca tcagcaacag
     6001 cgtaatggct atttatgggc gccaggctta acgctgtgga agtcagcaat cctgaaacct
     6061 ttcttgcgta ccgcgacaaa caggctgagt tattcacgtc gctgtactgc tgccaccgcc
     6121 tgcgtggtat ggggtgtaaa aggagaacag caatggcagg tcgaagcgca gcgaaaatca
     6181 ctgccattat ggcgaatgga agatggtttt ctgcgttcat ccggacttgg ctcggacctg
     6241 ctgccatcgc tatcgctggt gctggataaa cgcggaattt actatgacgc cacgcgcccc
     6301 agcgacctgg aagtgctgct taatcacagc cagttaacgc tggcgcagca gatgcgagct
     6361 gagaagttgc ggcaacgact ggttgaaagc aaactgagta agtacaactt gggagccgat
     6421 ttctctctgc ctgccgaagc caaaggtaaa aaagttatcc tggtgccggg tcaggtagag
     6481 gacgattcct ctattaaaac aggcactatc tcaattaaga gcaaccttga gttattacgc
     6541 acagtacgcg agcgtaatcc gcacgcctac attatttata aaccgcaccc ggatgtactg
     6601 gtggggaatc gcaagggcga tattccggca gaactgattg ctgaacttgc tgattatcag
     6661 gcactggacg cagatattat tcaatgcatt cagcacgcag atgaagtgca taccatgacg
     6721 tcgctgtcgg ggtttgaagc gttattacat ggtaagcacg tacattgtta cggcctgccc
     6781 ttctatgctg gttggggttt aaccttcgat gaacatcact gcgggcgtcg tgagcgagaa
     6841 ttaacgttag cggatttgct atatcaggcg ctgattgttt atccaactta tatccaccca
     6901 acacggctac aacctattac tgttgaagag gcagcggaat atttgatcca gacgtcgcgc
     6961 aagccgatgt ttattacccg aaaaaaagcg ggatgggtaa tacgctatta ccgcaaatta
     7021 attatgttct gcaaggttag atttggctaa acaatttcat agttgactat tacgcaaatt
     7081 atttatgcaa ggtaatgcac taaccgtttt attatccggt aaaaaatatc tgctattgca
     7141 gggaccgatg ggaccctttt tcagtgatgt agccgagtgg ctggagtcat taggccgtaa
     7201 cgctgtgaat gttgtattca atggagggga ttgtttttac tgtcgccacc gacattatca
     7261 ggcttactac cagacaccga aagagtttcc cggttggtta cgggatctcc accggcaata
     7321 tgactttgat accatcctct gctttggtga ctgccgccca ttacacaagg aagcaaaata
     7381 ttgggcaaag tcgaaaggta tccgctttct ggcatttgag gaagggtatt tacgcccgca
     7441 gtttattacc gttgaagaag gcggagttaa cgcatattca tcgctaccgc gcgatccgga
     7501 tttttatcgt aagttaccag atatgcctgc gccgcacgtt gagaacttaa aaccttcaac
     7561 gatgaaacgt ataggtcatg cgatgtggta ttacctgatg ggctggcatt accgccatga
     7621 gttccctcgc taccgccacc ataaatcgtt ttccccctgg tatgaagcac gttgctgggt
     7681 tcgttcatac tggcgcaagc aactttacaa ggtaacacag cgtaaggtat tgccgaggtt
     7741 aatgaacgag ttggaccaac tttattatct tgccgttttg caggtatata acgatagcca
     7801 gattcgtaac cacagtaatt ataacgatgt gcgtgactat attaatgaag tcatgtactc
     7861 attttcacgt aaagcgccga aagaaagtta tttggtgatc aaacatcatc cgatggatcg
     7921 tggtcacaga ctctatcgac cattaattaa gcggttgagt aaggaatatg gcttaggtgg
     7981 gagagtcatt tatgtgcacg atctcccgat gccgggacta ttacgccatg caaaagcggt
     8041 ggtgacaatt aacagtacgg tgggaatctc tgcactgatt cataacaaac cacttaaagt
     8101 gatgggcaat gccctctacg acatcaaggg cttgacatat caagggcatt tacaccagtt
     8161 ctggcaggct gattttaaac cagatatgaa actgtttaag aagtttcgtg gatatatatt
     8221 gatgaagaca cagcttaatg cagtttatta tgggattttt aattttagaa aaataacgaa
     8281 ttaataaaac atctataata caaacaacgg ttgttgtata ttatttattg aattcaatca
     8341 tttttatact taatgttttt gtaaataata agttgactgt gttgtactat attaatatag
     8401 gcgtttatat tcatatcttt aagagtaagc tattagatgt aaattacttt ggtcaggtcc
     8461 ttcacagcag aaacagaatg tcatgaaaga taaatttacg cagtcacttg atcaactgct
     8521 tagttttgcg tcttaaagga ttgtaactat ggcaactgtg gagcgaaaaa tcattgggca
     8581 cgggaaaagt gcggtcatat agaaaagtgc tctctattca gcatctcatg gtttatcagc
     8641 gaagacagaa aaaaattata acgatgcgca ggaatttgtg gaggtttgct aatatagagc
     8701 gattcgagca tccctcgtta aatctctcga accgcagtat attcagacat ttttaaaata
     8761 cgccagatga ttacgacaga aagaacacaa catataattc atgttcgctg tcctcttgct
     8821 gaatacggaa ttgtcttcaa taaaggtgct acacattatg gtgagagcta ccacggtttc
     8881 ttgaggatgc agaaaatgga tttaccgata tcgtgcaatt ttgtatcggt agtatgtccg
     8941 actcatgtct catggataat gaaccatata ttataactgc gggtagaaaa atgcgcatct
     9001 aacgactttg cctggacgtg tcgattaact gttcctgggt ttgctactct tgtcagtcag
     9061 acgataaaag tgagcataaa tatataggat tacatatcaa gtgaaaagaa gtggatttga
     9121 agttcagaag gtgacagtag aggcattatt tctacgagaa atacgaacac gttttggtaa
     9181 gtttcgtctg ggatatctgt gggcaatcct ggaacccgct gctcatttgc tggtgctgtt
     9241 aggtattttt ggctatatta tgcatcgtac gatgccaggt atctcgttcc cggtattttt
     9301 acttaatggt ttgattccct tttttatctt taatagtatc agtaatcggt ctgtggaagc
     9361 tattgatgca aaccaggggc tttttaatta tcggccagta aagcccatgg atacaataat
     9421 agccagaacc ctgcttgaga cactaattta cattatagtt tatgtgttgc ttatgcttat
     9481 tgtctggatg tcaggagaat attttgaaat aataagttta ttacaactgg tacttgtatg
     9541 gactcttctg attattattt catgtagtgt cggattaata tttatggtcg ttggtaaaac
     9601 tttccctgaa atgcaaaaga ttctaccgat atttcttaaa ccgctgtatt tcatttcctg
     9661 tatcatgttc cctctacatt cgatcccaca gcaatactgg acatatctac tctggaaccc
     9721 attagtccat gtcgtagagt taagtcgtga ggcagttatg ccgggctata tcagcgaagg
     9781 cgtgagtttg aactatctgg caatgttcac tctggtcacc ctgttcatcg gtctggcgct
     9841 ataccgaacg cgtgaagagg caatgctgac atcatgatta agattgagaa tctgacgaag
     9901 tcatatcgca cgtcaacagg tcgacactat gtgtttaagg atttaaatat cgaactccct
     9961 tcaggaaaaa gtgttgcctt tattggtcgt aatggtgcag gtaaatcaac tttactgaga
    10021 atgattggcg gtattgaccg ccccgataga ggaaagatca tcaccaataa aacgatatca
    10081 tggcccattg ggctcgccgg tggattccag ggcagtttaa ccgggcgaga aaacgtaaaa
    10141 tttgttgcac ggttatatgc gaagccagaa gaactgaaag ataaaattga gtttgtcgaa
    10201 gaatttgccg aactcggtaa gtactttgat atgccgatca aaacttactc atctggtatg
    10261 cgatctcgcc tgggttttgg tttaagcatg gcatttaagt ttgattatta tattgtcgat
    10321 gaagtaacgg cagtcggtga tgccaggttt aaagaaaaat gtgcacatta ctttaaagaa
    10381 aggcataaag agtctagttt tttaatggtt tctcatagtt taaattcttt gaaagagtat
    10441 tgcgatgtgg ttatatatat taaaagtttt aatgaagtaa aatactacag tgtagtaact
    10501 gatgcgctgg cagaatatat tatcgacact aaaatacaag tataaacttt ccaagtaaca
    10561 aggcgttatt atgtctataa ttgtaaatca tgttatgtct tctgatatag taagtggaat
    10621 atttacagat cttctctctt attacaaaag atattgtgat agagatatat caattgttga
    10681 aagtagcaac gctattgata atgctgatgt ctatcactat catcggcctc atttagaaag
    10741 cagacttaag gaaaactcag tagtaacaat acatcatgac ttgtatgata cagacccatg
    10801 gttggattat gataagtttc atccccgtta tatggaagca aaaaaaatac tgtgccttaa
    10861 ctcctctcag caaaattatt taaacaataa aggcttaatg aatactgaaa ttataccaca
    10921 tggttacaat tctgacatct tttgctcagt aggggcgcaa aaaaaaataa aaaataaagt
    10981 ttgcattggt tttatatcaa agagatatgg acgaaaagtg aaaggagaag cttatcttta
    11041 tgagattttt aaaagattag attctaaaag atacagattc atctttgtag gagtcgatag
//
LOCUS       DAZGXZ010000157        11071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000157 DAZGXZ010000000
VERSION     DAZGXZ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.157"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            67..609
                     /gene="yehD"
                     /locus_tag="ACHKJF_004458"
     CDS             67..609
                     /gene="yehD"
                     /locus_tag="ACHKJF_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951707.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSRMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            689..1363
                     /gene="yehC"
                     /locus_tag="ACHKJF_004459"
     CDS             689..1363
                     /gene="yehC"
                     /locus_tag="ACHKJF_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0951708.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            1379..3859
                     /gene="yehB"
                     /locus_tag="ACHKJF_004460"
     CDS             1379..3859
                     /gene="yehB"
                     /locus_tag="ACHKJF_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0951709.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEEAFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAIADLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGRSCTITFGKEIDESRNYICQ"
     gene            3873..4907
                     /gene="yehA"
                     /locus_tag="ACHKJF_004461"
     CDS             3873..4907
                     /gene="yehA"
                     /locus_tag="ACHKJF_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP0951710.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(4989..5327)
                     /gene="rcnB"
                     /locus_tag="ACHKJF_004462"
     CDS             complement(4989..5327)
                     /gene="rcnB"
                     /locus_tag="ACHKJF_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP0951711.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5546..6370)
                     /gene="rcnA"
                     /locus_tag="ACHKJF_004463"
     CDS             complement(5546..6370)
                     /gene="rcnA"
                     /locus_tag="ACHKJF_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP0951712.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHVEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6491..6763
                     /gene="rcnR"
                     /locus_tag="ACHKJF_004464"
     CDS             6491..6763
                     /gene="rcnR"
                     /locus_tag="ACHKJF_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP0951713.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            7093..8313
                     /locus_tag="ACHKJF_004465"
     CDS             7093..8313
                     /locus_tag="ACHKJF_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0951714.1"
                     /translation="MGKALNKLSDSTLKKLVAVQAEKERFYSDGGGLEIKHSKGGKLT
                     WYFRYRTGGREVAAERLKLGAYPELSLKAAREKRAQCRAWLAEGKNPRYELCATVQEA
                     LKPVTVKEAINYWLDEYAKDNRKDYIKLVQRMDKHIICHIGAIPLDKCDTRQWIACFD
                     RVRKKAPVAAGHVMQTCKQALKFCRRRRYAFSNALDDLIVSDVGKRAEIRERVHSNSE
                     LKEILRAIDGDVFAPYYSALMRLLIVFGCRTAEVRLSEIKEWDLKEMLWTVPKEHSKT
                     KVTIFRPIPDGILPFIQKLVEQNRHTGLLLGELKKDTTVAQYGRNAHKRLNQEHWTLH
                     DFRHTFTTMLNDLGIDPHIVEHITAHQMPGQQKTYNHSRYLQAKRDALNLWVERLDVL
                     AGNHENIVMLKVAK"
     gene            8310..9104
                     /locus_tag="ACHKJF_004466"
     CDS             8310..9104
                     /locus_tag="ACHKJF_004466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951715.1"
                     /translation="MKDFPRIYYTLPMAAKKLGCTVDDILHLGATNRLEICAYIGEND
                     LLISENKCSARVIFEDDSESYYVEQIKERNVIDSDMYKISYLHYNDDYVEDTDTGIFR
                     LPGWYANYLKGFFAIPSQYLVDVELSGPHNAVDVKPQHLYTIKAHYKKLYLAGIWGLF
                     IPEERLVVFENELSNFDYAAKPVYGGGDRESPKTIAKKAEIIPALIKLIPEMQDVDLE
                     ETPVTKIAEMIEVIAAQRGVELPSTHRQTWQKYLGRDIGDVPKRQK"
     gene            9196..9420
                     /locus_tag="ACHKJF_004467"
     CDS             9196..9420
                     /locus_tag="ACHKJF_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0951716.1"
                     /translation="MNQNTQFQSLSDRVIREDECRKLTGVCRTTRYELEKKGRFPSRL
                     NLGGRSVGWLLSEVMEWVKSRDRINSGKAV"
     gene            complement(9532..9705)
                     /locus_tag="ACHKJF_004468"
     CDS             complement(9532..9705)
                     /locus_tag="ACHKJF_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000130015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951717.1"
                     /translation="MTNEESTEKTSPARKRRRRKKEHESERFAPCAFALEQFLKEYRE
                     TRSLQVWQRTEPD"
     gene            complement(9763..9960)
                     /locus_tag="ACHKJF_004469"
     CDS             complement(9763..9960)
                     /locus_tag="ACHKJF_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0951718.1"
                     /translation="MSSGNTNNKSAKKNIRFPHELIDGIDASVEQEKLTNPSANFSAW
                     VLDACGRKLKYEQRRKAKESE"
     gene            10159..10356
                     /locus_tag="ACHKJF_004470"
     CDS             10159..10356
                     /locus_tag="ACHKJF_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0951719.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQEVRHD"
     gene            10349..10561
                     /locus_tag="ACHKJF_004471"
     CDS             10349..10561
                     /locus_tag="ACHKJF_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951720.1"
                     /translation="MINLSLTDLNRIQFREKFTGQLLVNVDKGRVVCNYHLPDEAIVA
                     TRESLQEITKRAGMINTNIQGMLYAQ"
     gene            10551..10811
                     /locus_tag="ACHKJF_004472"
     CDS             10551..10811
                     /locus_tag="ACHKJF_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951721.1"
                     /translation="MHNKTTPDAAAEAIKTLMHALIDISVIADRAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGGLCVMIVLIP"
     gene            10784..11017
                     /locus_tag="ACHKJF_004473"
     CDS             10784..11017
                     /locus_tag="ACHKJF_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951722.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
BASE COUNT         3169 a         2210 c         2714 g         2978 t
ORIGIN      
        1 gagaaatttg tttccaagag atacagactc ttaacaacca aaaaaaattt tattaaggaa
       61 catattatga aacgttcaat tattgctgcc gctgtctttt cttctttttt tatgagcgct
      121 ggagtatttg ctgcagacgt tgataccgga acattaacta ttaaggggaa tattgcagaa
      181 tctccgtgta aattcgaagc gggtggtgat tcagtaagta ttaatatgcc gactgtacca
      241 accactgtct ttgaaggtaa agctaaatat tctacctatg atgatgcagt cggtgtaacc
      301 agcagaatgt taaaaattag ctgcccgaaa gaagttgctg gtgtaaaact ctcgttgatt
      361 accaacgata aaataaccgg taacgataag gcgatagcca gtagcaacga taccgtgggt
      421 tactatctct atttaggtga taacagcgat gtcctggatg tttctgcacc ttttaacatt
      481 gagagttata aaacagcgga aggtcaatat gctattccgt ttaaagcaaa atacctgaaa
      541 ctgacagata actcagtgca atcaggtgat gtgttatctt ctctggttat gcgtgtggcg
      601 caggattaat aaataaaatt gtaagtttct ccaggtaata aacatggccg ctatcccatg
      661 gcggcctttt aatttaagag gcattaaaat gaaaggatta ttatctttac tcattttttc
      721 tatggtcctt cctgcacatg ccggaattgt tatctacggg acgcgcatta tttacccggc
      781 agaaaataaa gaagttatgg tgcagttgat gaaccaggga aaccgttctt cgctgctgca
      841 ggcgtggatt gatgatggcg atacgtcgtt accaccagaa aaaattcagg ttcctttcat
      901 gttaacgcca ccagtggcaa aaataggggc aaattccggg cagcaagtaa aaatcaaaat
      961 tatgccgaat aaactgccca ctaataaaga aagcattttt tatctgaatg ttctggatat
     1021 tccaccaaat agtccagagc aagaaggcaa aaatgcactg aagtttgcga tgcaaaacag
     1081 aattaagttg ttttaccggc cagcgggtat tgctccggta aataaagcga cctttaaaaa
     1141 gttactggta aatcgcagtg gcaatggatt ggtgataaaa aatgactcag ctaattgggt
     1201 gacgatttcg gatgtcaaag ctaataatgt taaagtcaat tatgaaacta ttatgattgc
     1261 ccccttagaa agtcagagtg ttaatgtcaa aagtaataat gcaaataact ggtatctgac
     1321 cattatcgat gaccacggca actatattag tgacaaaatt taattgcagg agctgcctat
     1381 gttgagaatg accccacttg catcagcaat cgtagcgtta ttgctcggca ttgaagctta
     1441 tgcagctgaa gaagcctttg atacccattt tatgataggt ggaatgaaag accagcaggt
     1501 tgcaaatatt cgtcttgatg ataatcaacc cttaccgggg cagtatgaca tcgatattta
     1561 tgtcaataag caatggcgcg ggaaatatga gattattgtt aaagacaacc cgcaagaaac
     1621 atgtttatca atagaagtta tcaagcggtt aggcattaat agcgataact tcgccagcgg
     1681 taagcaatgt ttaacatttg agcaacttgt tcagggtggg agctatacct gggatatcgg
     1741 ggtttttcgt ctcgatttca gtgtcccgca ggcctgggtg gaagaactgg aaagtggcta
     1801 tgttccaccg gaaaactggg agcggggtat taatgcgttt tatacctctt attatgtgag
     1861 tcagtattac agtgactata aagcgtcggg taatagtaag agtacatatg tacgttttaa
     1921 cagcgggtta aatttactgg ggtggcaact gcattctgat gccagtttca gtaaaacaaa
     1981 taacaatcca ggggtgtgga aaagcaatac cctgtatctg gaacgtggat ttgcccaact
     2041 tctcggcacg cttcgcgtgg gtgatatgta cacatcaagc gatatttttg attctgttcg
     2101 cttcagcggt gtgcggttgt ttcgtgatat gcagatgttg cctaactcga aacaaaattt
     2161 tacgccacgg gtgcagggga ttgctcagag taatgcgctg gtaactattg aacagaacgg
     2221 ttttgtggtt tatcagaaag aggttcctcc tggcccgttc gcgattgcag atttgcagtt
     2281 ggccggtggt ggagcagatc ttgatgtcag cgtgaaagag gcggacggct cggtaaccac
     2341 ctatctggtg ccttatgcag cagtgccaaa tatgctgcaa cccggcgtat cgaaatatga
     2401 ttttgcggcg ggtcgtagcc atattgaagg ggcgagcaaa caaagtgatt ttgtccaggc
     2461 gggttatcag tatggtttta ataatttatt gacgctgtat ggtggctcga tggtcgcgaa
     2521 taattattac gcgtttactt tggggactgg ctggaataca cgcattggtg ccatttccgt
     2581 cgatgccacg aagtcgcata gtaaacaaga caacggcgat gtgtttgacg ggcaaagtta
     2641 tcaaattgcc tacaacaaat ttgtgagcca aacgtcgacg cgttttggtc tggcggcctg
     2701 gcgttattca tcgcgtgatt accggacatt taatgatcac gtttgggcaa acaataaaga
     2761 taattatcgc cgtgatgaaa acgatgtcta tgacattgcc gattattacc agaacgattt
     2821 tggccgtaaa aatagcttct ctgccaatat gagccagtca ttgccagaag gctgggggtc
     2881 tgtgtcctta agtacgttat ggcgagatta ctgggggcgt agcggcagca gtaaggatta
     2941 tcagttgagt tattccaata actggcggcg gataagctat atcctcgcgg caagccaggc
     3001 gtatgacgag aatcatcatg aagagaaacg ttttaatatt tttatatcga ttccctttga
     3061 ttggggtgat gacgttacga cgcctcgtcg gcaaatatat atgtctaact caacgacgtt
     3121 tgatgatcag gggtttgcct caaataatac gggattatca ggaaccgttg gaagccggga
     3181 tcagtttaac tatggggtca acctgagtta tcagaatcag ggaaatgaaa cgacagctgg
     3241 ggcgaatttg acctggaacg cgccggttgc gacagtgaat ggcagttata gtcagtcgag
     3301 tacttatcga caggctggag ccagtgtttc agggggcatt gtcgcctggt cggggggcgt
     3361 taatctggcg aaccgtcttt cagaaacgtt tgctgtgatg aatgcgccag gaattaaaga
     3421 tgcttatgtc aatgggcaaa aatatcgcac aacaaaccgt aatggagtgg tggtatacga
     3481 cggaatgaca ccttatcggg aaaatcacct gatgctggat gtgtcgcaaa gcgatagcga
     3541 agcagaatta cgtggcaacc ggaaaattgc cgccccttat cgcggcgcgg ttgtactggt
     3601 taattttgat accgatcagc gcaagccctg gtttataaaa gcgttaagag cggatgggca
     3661 accattaatg tttggttatg aagtcaatga tatccatggt cataatattg gcgttgtcgg
     3721 tcagggaagc cagttattta ttcgcaccaa tgaagtaccg ccatcggtta atgtagcaat
     3781 tgataagcaa caaggacgtt catgcacaat caccttcggt aaagagattg atgaaagtag
     3841 aaactatatt tgccagtaat agtcaggtgg ttatgaatat tcgtataatg ttatttatag
     3901 taatgatgat ggttatgcct gtgagctatg cggcatgtta tagtgagtta tctgttcagc
     3961 acaatttggt tgttcagggg gattttgcac ttactcaaac acaaatggcg acatatgagc
     4021 ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg agcccgtcgg
     4081 atattattgt tggactttat aacgatacca ttaaattaaa tttacatttt gaatggacca
     4141 ataaaaacaa catcacgtta tcaaataatc agaccagttt caccagtggt tattcggtaa
     4201 cggtgacacc tgcggccagt aatgcaaaag tgaatgtttc tgcggggggc ggcggttcag
     4261 tgatgattaa tggagttgcg acattatcca gtgcttcatc atcgacacgc gggagtgccg
     4321 cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt gtaaatagct
     4381 acagaaatgc attggcacaa aatgcaggtg tctattcctt taatctgaca ttgtcataca
     4441 acccgataac cacaacctgc aaaccggacg atttattaat tactttagac agtattcccg
     4501 tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaacaa ggggatatta
     4561 ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa atgcaggtgt
     4621 atttatcaag ttctgacttg ttaaccaaca gcaacacaat actgaaaggt gcggaagata
     4681 atggcgtagg atttattctt gaaagtaatg gttcgtcagt cacactttta aatatcacta
     4741 acagcagtaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa cttacagata
     4801 caacggtttc aattccgata actgccagtt actacgtcta cgatacaaac aaagttaaat
     4861 ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa caagcaagac
     4921 gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc atgatgaggg
     4981 ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat tttaccgtcg
     5041 gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc ggcatcaggc
     5101 gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg gtacatctct
     5161 gggacggtat cgcctggttt gaaatgctta aagtcagcaa tgaaactgct taactcatac
     5221 ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca ggcggcactg
     5281 gtaaccagca aaagcgcacc cagcaacatc ttatttttaa tggtcataaa ctctccactt
     5341 tggggatctg taatatcagt cgcatatctt tcctgccata caatataact gacaagcgat
     5401 attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc cctaaaagca
     5461 tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct gggctgaaga
     5521 taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca tgtacgccca
     5581 tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt ttagcgagag
     5641 tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg ccaacgccta
     5701 cggtgataag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt gcgcccagtg
     5761 tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg caggggataa
     5821 ggccaccggt taagccaaat aacaaaattt gccagttggt gacctctcta ccatcaaagc
     5881 gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg acatgttcgt
     5941 gatggtgatg atgtccatgg tcgtggtgat cttcgtgatc gtggtgatga tgttcatgat
     6001 catactcgtg catattctcc agccagttgc gttcgccgcg ccaggtacgc cagaacatcc
     6061 agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt tctgctgatt
     6121 gagcagtaaa gcgcttgctg atcaccatcc cgccaaaggc aattaaccag accactgcgg
     6181 tatgcgaaat agttgctgcc agtccgagca tcaccgcttg tttaatggtg cctttgatgg
     6241 cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca tgaagcgcac
     6301 caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga agagttgtaa
     6361 attcggttat gataataatt cttagtatta attcggcaat ctgattctac tcccccccag
     6421 tacctgatac taccccctag tagattaata gtgctatgat ttttcatatt cttgttaaca
     6481 aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc gtgccagtaa
     6541 gattcagggc caggtcgtgg cgctcaagaa aatgctcgac gagccgcacg aatgcgctgc
     6601 agttttacaa cagattgctg ctatccgtgg cgcggtaaac ggtctgatgc gggaagtgat
     6661 taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac gtgaagaaga
     6721 tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta acgcgattgt
     6781 aaaactgccg tttttcctcg tttacaacgc gtgcgctgga cattaccatc ctcctctgcg
     6841 atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg agaaataccc
     6901 gtattttctc atttagtgtt ttttgtggtt cggggttgtt cgtggttgtt tgtgtatgtg
     6961 tttgatatgt attgaattat tgtgttttct tgtttgttgt tgttcgtcgt tgttcgtaga
     7021 gcttcaacga aatgtgtggt cagttgtgtg gtcagttttt tgaggttgga ggacaggaaa
     7081 gaggatctga tagtgggaaa ggcactaaac aaactgagcg attcaacgtt aaaaaaattg
     7141 gtggctgtcc aggcagaaaa agagcgtttt tactccgacg ggggcgggct ggagattaaa
     7201 cactcaaagg gcggcaaatt aacctggtat ttccggtacc gaacgggagg gcgtgaggtt
     7261 gccgcagagc ggttaaagct gggggcttac cctgaattgt cgctgaaagc tgcaagggaa
     7321 aaacgcgcac agtgccgggc atggctggct gagggtaaaa acccccgtta tgagttgtgc
     7381 gccacagtac aggaagcgct aaaacccgtt acggtgaagg aggcgattaa ctactggctg
     7441 gatgaatatg cgaaggataa ccgcaaagac tacataaagc tggtgcaacg tatggataag
     7501 cacatcattt gccatatagg ggcaattcct cttgataaat gtgatacaag gcagtggatc
     7561 gcatgttttg accgtgtacg aaaaaaagca ccagtagcag cagggcacgt catgcaaacc
     7621 tgcaaacagg cgctaaagtt ctgccgcagg aggcgctacg cgttcagtaa tgccctggac
     7681 gatttgatcg tcagcgatgt gggtaagaga gcagaaattc gcgagagggt gcacagtaac
     7741 agcgaattaa aggaaatttt acgcgctatc gatggtgatg tgttcgcgcc ttactacagc
     7801 gcgttaatgc gcttgctgat tgtgttcggg tgcagaacgg cagaggtcag actttcagag
     7861 atcaaagaat gggatctgaa agaaatgttg tggacagtgc caaaagagca cagcaaaacg
     7921 aaggtaacga tattcaggcc aatccccgat ggcattttgc cgttcattca gaagctggtg
     7981 gagcagaacc gacatacagg gttattgctc ggagaactga aaaaagatac cacggtagcg
     8041 caatatgggc gaaatgcgca taagcggctt aaccaggaac actggacgct gcatgacttt
     8101 cgccacacgt ttacaacgat gctgaacgat ttaggtatcg atccgcatat cgtggagcac
     8161 atcacagcgc atcagatgcc cggtcagcaa aaaacctata accattcacg ctatttacag
     8221 gcgaaacggg acgctctgaa tctatgggtt gagcgtctcg atgtgctggc tggaaatcat
     8281 gaaaatattg ttatgctgaa ggtggctaaa tgaaagattt tcctcgtata tattatactt
     8341 tgcctatggc agcaaaaaaa ctcggctgca cggtggatga tattctacat cttggggcaa
     8401 ccaatagact tgaaatatgt gcatatattg gtgaaaacga cttattaata agtgagaata
     8461 agtgctctgc tagagtgatt tttgaagatg atagcgaaag ttattatgtt gaacagataa
     8521 aagaacgaaa tgtaatagat agtgatatgt ataagatatc ttatcttcat tacaatgatg
     8581 attatgttga ggataccgac acagggatat tcagactgcc aggttggtat gcaaattatt
     8641 taaagggttt ttttgcaatt ccgtcgcagt atcttgtgga tgttgaatta tcaggtccgc
     8701 acaatgctgt tgacgtcaaa ccacaacatc tttacaccat aaaagcacat tacaaaaagc
     8761 tatatttagc tggaatttgg gggttgttta ttccggagga aagattagtc gttttcgaaa
     8821 atgaactttc gaattttgat tatgcggcaa aacctgtata tggcggaggt gatagagaat
     8881 ctccgaaaac catagctaaa aaagccgaga taatacctgc actcataaaa ctgattcctg
     8941 agatgcagga tgtggatttg gaggagacac cagtaaccaa aattgcagaa atgatagagg
     9001 ttatcgctgc gcaaagaggg gttgagctac ccagtactca caggcagaca tggcagaaat
     9061 atttggggcg tgatattggt gacgttccga aacgtcaaaa gtgatatcac gtaccgatag
     9121 tgtgatatca cacaaacggc gttttaccac cgtctagaat cgtcctcgtt cggcaacgtt
     9181 cgaggacgtt tttttatgaa tcaaaataca caatttcaat cactatcaga tcgcgtaatc
     9241 cgtgaggatg aatgtcgtaa gttaactgga gtgtgtcgta ctacacgcta cgaactggag
     9301 aaaaaagggc gatttccgtc acgccttaat ctgggcggtc gttctgtagg ttggctgctg
     9361 tccgaggtta tggaatgggt taaaagccgg gatcgcatta attcaggtaa ggcagtgtaa
     9421 aggggagcat atgacacata aaacaaaggc gaccgggggc ggtcgccaat gggaaagcac
     9481 caaacataag cacggggatg atagccgcta tcaggctggt gggcaatgca atcagtctgg
     9541 ttcagttcgt tgccatacct gcaatgagcg cgtttccctg tactctttaa ggaattgctc
     9601 aagggcaaaa gcacatggcg cgaatcgttc tgattcatgc tctttctttc tgcgtcgtct
     9661 ttttcgtgca ggtgatgttt tttcggttga ttcttcgttg gtcattgtgt gcgcctgtaa
     9721 agcaatgcgc cgtagtacct cacaccacgg cgctgatagt gattattctg attctttggc
     9781 cttccggcgc tgttcatatt ttaatttgcg tccacaggca tctaagaccc atgcggaaaa
     9841 attagcacta ggattagtta atttttcttg ttcaacgctg gcatcaatcc catctataag
     9901 ctcatgtggg aatcgaatat ttttctttgc tgatttgttg tttgtgttac cgctagacat
     9961 tgatgcacct cacttaaatg gactttatgg gcgcacacaa tacagcaaaa aaaatgagat
    10021 gttaagtatt gacatgtgcg cacacatgat tttaatctgt gcgcacagtt tgagttttgt
    10081 cactcaaatt aagcaacgcc ccgcagtgct cgcaacacat gcagggcgtc taaccaacaa
    10141 cgtaaacgag gagccgttat ggttgccgta aatcatatac cacaccttgt acacacacaa
    10201 acggcctttg tgtggcgttt tctggcactg agtgccggag aatctcaaat catccacgta
    10261 accgcctgga cggaacgcga agcgcgtagc cgttgcccgt ccggttgtgt tgctgtattt
    10321 gccgcccgta ttcgtcagga ggtgcgccat gattaatctg tcacttactg accttaaccg
    10381 cattcagttt cgtgagaaat tcacagggca gctactggtc aatgtggata aaggtcgcgt
    10441 ggtgtgtaat taccacttac cagatgaagc aattgtcgcc acaagggaat cattacagga
    10501 aatcacaaaa cgtgccggaa tgattaacac gaatattcag gggatgcttt atgcacaata
    10561 aaaccacacc ggacgcagcc gccgaagcca tcaaaacgct gatgcacgcg ctgattgata
    10621 tttctgttat cgcggacagg gcacataagc acgccaccag cgaaacagaa tatgccgggg
    10681 ctttcgttcc tcattcgctg gcggttatgc aacttagtgc tgatatggcg ctgaatgagg
    10741 ccaaagctat cctgattgct gattgtgaaa atgggggggg gttatgcgtg atgatcgttt
    10801 taattccctg aaacaagaat tttccggcgt tcctgatgat gcggctgatg cgctttcgtc
    10861 aatgccagaa cttattagag cggctttttt cttactttcc acgagagaat ataaatcaac
    10921 ggggcttgat gtactgaata tcgccgccga ttatgcggaa tatgtggcag aggctcgtta
    10981 cagaagaaaa tttcctgagg atgtaagcca tgcgtgatat ttaccacgaa acaacagatc
    11041 gcgtatttct tgcactttct cacagtgaaa a
//
LOCUS       DAZGXZ010000158        11002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000158 DAZGXZ010000000
VERSION     DAZGXZ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.158"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(56..1885)
                     /gene="glmS"
                     /locus_tag="ACHKJF_004474"
     CDS             complement(56..1885)
                     /gene="glmS"
                     /locus_tag="ACHKJF_004474"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP0951723.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2047..3417)
                     /gene="glmU"
                     /locus_tag="ACHKJF_004475"
     CDS             complement(2047..3417)
                     /gene="glmU"
                     /locus_tag="ACHKJF_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP0951724.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3768..4187)
                     /locus_tag="ACHKJF_004476"
     CDS             complement(3768..4187)
                     /locus_tag="ACHKJF_004476"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP0951725.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4208..5590)
                     /gene="atpD"
                     /locus_tag="ACHKJF_004477"
     CDS             complement(4208..5590)
                     /gene="atpD"
                     /locus_tag="ACHKJF_004477"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP0951726.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5617..6480)
                     /gene="atpG"
                     /locus_tag="ACHKJF_004478"
     CDS             complement(5617..6480)
                     /gene="atpG"
                     /locus_tag="ACHKJF_004478"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP0951727.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YADTMRKVIGHLASGNLEYKHPYLEERDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6531..8072)
                     /gene="atpA"
                     /locus_tag="ACHKJF_004479"
     CDS             complement(6531..8072)
                     /gene="atpA"
                     /locus_tag="ACHKJF_004479"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP0951728.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8085..8618)
                     /gene="atpH"
                     /locus_tag="ACHKJF_004480"
     CDS             complement(8085..8618)
                     /gene="atpH"
                     /locus_tag="ACHKJF_004480"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP0951729.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8633..9103)
                     /gene="atpF"
                     /locus_tag="ACHKJF_004481"
     CDS             complement(8633..9103)
                     /gene="atpF"
                     /locus_tag="ACHKJF_004481"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP0951730.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9165..9404)
                     /gene="atpE"
                     /locus_tag="ACHKJF_004482"
     CDS             complement(9165..9404)
                     /gene="atpE"
                     /locus_tag="ACHKJF_004482"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP0951731.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9451..10266)
                     /gene="atpB"
                     /locus_tag="ACHKJF_004483"
     CDS             complement(9451..10266)
                     /gene="atpB"
                     /locus_tag="ACHKJF_004483"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP0951732.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIDGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10275..10655)
                     /gene="atpI"
                     /locus_tag="ACHKJF_004484"
     CDS             complement(10275..10655)
                     /gene="atpI"
                     /locus_tag="ACHKJF_004484"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP0951733.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2619 a         3007 c         2728 g         2648 t
ORIGIN      
        1 gtcagttata aaaaagggcc accggtcggc agcccttttt tatctatgat agtgattact
       61 caaccgtaac cgattttgcc aggttacgcg gctggtcaac gtcggtgcct ttgatcagcg
      121 cgacatggta agccagcagc tgcagcggaa cggtgtagaa gatcggtgca atcacctctt
      181 ccacatgcgg catctcgatg atgtgcatgt tatcgctact tacaaaaccc gcatcctgat
      241 cggcgaagac atacaactga ccgccacgcg cgcgaacttc ttcaatgttg gatttcagtt
      301 tttccaacag ttcgttgttc ggtgcaacga cgataaccgg catatccgcg tcaatcagcg
      361 ccagcgggcc gtgtttcagc tcgcctgcag cgtaggcttc agcgtgaatg taagagatct
      421 ctttcagttt caatgcgcct tccagcgcga ttgggtactg atcgccacgg cccaggaaca
      481 gcgcgtgatg tttgtcagag aaatcttctg ccagagcttc aatgcgtttg tcctgagaca
      541 gcatctgctc aatacggctc ggcagcgcct gcagaccatg cacgatgtca tgttcaatgg
      601 aggcatccag acctttcagg cgagacagct tcgccaccag catcaacagc acagttaact
      661 gagtggtgaa tgctttagtg gatgccacgc cgatttccgt acccgcgttg gtcatcagcg
      721 ccagatcgga ttcgcgcacc agagaagaac ccggaacgtt acagattgcc agcgaaccaa
      781 ggtaacccag ctctttcgac agacgcaggc ccgccagggt atccgcggtt tcgccagact
      841 gtgacaaggt tatcatcagg ctgttacgac gcacggcaga tttgcgatag cggaattcag
      901 aggcgatttc gacgtcgcac ggaatacctg ccagcgattc aaaccagtaa cgggaaacca
      961 taccggagtt ataagaagta ccacaggcga ggatctgaat atgctcaacc ttcgacagca
     1021 gttcgtcggc gttcggtccc agctcgctta aatcaacctg accgtggctg atgcgcccgg
     1081 taagggtgtt ttttatcgcg ttcggctgtt cgtagatctc tttctgcatg tagtgacggt
     1141 aaataccttt atcgcccgcg tcatattgca gattggattc gatatcctga cgttttactt
     1201 ccgcgccagt tttatcgaag atgtttaccg aacggcgagt aatttccgca atatcgccct
     1261 cttcaaggaa gataaagcga cgggtcaccg gcaacagcgc cagctggtca gaagcgataa
     1321 agttttcgcc catacccagg ccaatcacca gcggactacc agaacgtgcc gccagcaggg
     1381 tatccgggtg acgggagtcc atgatcactg taccgtacgc accacgcagt tgcgggatag
     1441 cacgcagaac agcctcacgc agagtaccgc cctgtttcag ctcccagttc accagatggg
     1501 caattacttc ggtgtctgtt tcagaaacga aggtatagcc acgcgctttt aactcttcgc
     1561 gcagcggttc atggttttcg atgatgccgt tatgcaccac cacaatgtgt tcagaaacat
     1621 gcggatgcgc attcacttct gaaggttcac cgtgggtcgc ccagcgagta tgagcaatac
     1681 ccgtaccgcc atgcagagga tgttcttccg ctgcctgagc cagcatctgg actttaccga
     1741 ggcgacgcag gcgggtcata tgaccttccg catcaacaac ggccagaccg gcagagtcat
     1801 atccgcggta ttccagacga cgtaaacctt caagaaggat ttctgctaca tcacgttgcg
     1861 cgatcgcgcc aacaattcca cacatagttt ttgattccga tttatatcgt tgtcggtcaa
     1921 cctgtatgcc cgttttttcg ggcgccccga gccttgtaga gagtggggtt atttttatag
     1981 ttactgcttg tgggagggag attatgttat ctcctcatcc catgtgaccg gattacccgg
     2041 ccagaatcac tttttcttta ccggacgacg ccagccttct ttctgagtct gcggcacacg
     2101 gctgatagct aatgcatttt cgccgacatt acgcgtcaca gttgtacccg cagcaatggt
     2161 cgcgcctttg cctactgtta ccggggccac cagctgagtg tcggaaccga caaacacatc
     2221 gtcgccgata atggtcttaa atttattcgc accatcgtag ttgcaggtaa tggttcccgc
     2281 gccgatgtta acgttatcgc caatttccgc atcgcctagg taagtcagat gaccagcttt
     2341 cgagccttta cccagacgcg cttttttcat ctcaacaaag ttaccgacgt gtgcaccttc
     2401 cagcaactca gcaccaggac gcagtcgggc aaacgggcca atagtacagg ccgctgccag
     2461 attcgcgtct tccacaacgg tatacggact gatttcgcaa tcatcgccaa tcacgctgtt
     2521 tttaatcacg caaccggtgc cgattttcac gcgatgaccg agagtcacgt tgccctcgat
     2581 gataacgtta gtatcaattt caacatcgcg cccgtgagta agcgtaccgc gcaaatcaaa
     2641 acgcgccgga tcgcgcagca taacgcctgc taacagcagt ttttcagcct gttcggactg
     2701 gtaaacgcgc tccagacggg agagttgcag gcggttattc acaccttcta cttcgcttaa
     2761 acgttgcgga tgaacggcga cgatttcacg cccttcctga tacgccagcg caataatgtc
     2821 ggtgatgtag tattcgccct gagcattatt gttggtcagc ttcgccagcc agcgtttcat
     2881 atctgcgccg ttagcgatca gaatgccggt gttgatctcc tgaatctgac gctgctcgtc
     2941 ggtggcatct ttgtgctcaa caatgccggt aactttgcca ttttcacggg tgatacgtcc
     3001 ataaccggtc ggatcatcca gtttcaccgt cagcagacca atgccaccct gcggtttagc
     3061 atcacgcagg cgctggagtg tttcgacaga gatcagcggc acgtcgccgt agagcattaa
     3121 aatgtcttca tcatcggcaa agaaaggtgc ggcctgctgc attgcatgac ccgtacccag
     3181 ctgctctgcc tgaagcaccc agttaaggtt gtcgtctttc agcgcctgtt taagcagatc
     3241 gccgccgtga ccgtacacca ggtgaacgtg cgctgcgcct aattcattcg cagcatcaat
     3301 gacatgctga accatcgctt tcccggcaag ggtatgcagc actttcggaa gatcggaata
     3361 catgcgcgtg cctttgcctg cggcaaggat cactacgctc atagcattat tcaacatacg
     3421 cgtcctgact gtaatttgag aacgaattta aaccgcttca ccttgaaaaa actacatttt
     3481 tttcatcgtg aaatggacag aggataaatt gttcaataac ggattatccc cgctgacgaa
     3541 agtgccattt tcgaccatcg tcacgccttt tgtctgcaga aaattaaggc agtaccttca
     3601 tttaagcgtc aggtggatgt ttttgctctt atttcgatca atgaataaac gggaaaacag
     3661 gggtgtttta tatcagcagg atctatgtga acgctcttca ggacggatca cgcgtaaaaa
     3721 aaagccagcc tgtttccaga ctggcttttg tgcttttcaa gccggtgtta catcgctttt
     3781 ttggtcaact cgataacgcg cagctgcgcg atcgctttgg ccagttccgc agacgcctga
     3841 gcgtaatcta cgtcgccgtg agagctgcta atgtgctctt cagccttacg tttcgcttcc
     3901 atggctcgcg cttcgtcgag atcctggccg cgaattgcgg tgtcggccag aacggtcacg
     3961 ttgccaggct gcacttcaag aatgccgcca gacagataga taaactcttc gtgaccgtgc
     4021 tgtttcacga tgcgaatcat accaggctta atggcggtga gcagcggtgc gtggccaggg
     4081 tagatcccca gttcaccttc gctacccgtt acctggattt tctcgaccag accagagaac
     4141 atttgttgct ctgcgctgac gacgtccagg tggtaagtca ttgccatatc accctccgat
     4201 taaggcgtta aagttttttg gctttttcca cagcttcttc gatggaaccg accatgtaga
     4261 acgcctgctc cggcaggtga tcgtattcgc cttccatgat gcctttaaag ccacggatgg
     4321 tgtctttcag ggagacgtat ttacccggag aaccggtgaa tacttctgcc acgaagaacg
     4381 gctgggacag gaagcgctgg atcttacgag cacgcgctac caccagtttg tcttcttcag
     4441 acagttcatc catacccagg atggcgatga tgtctttcag ttcctgataa cgttgcagga
     4501 tggactgaac gccacgcgcg gtgtcgtagt gttcctgacc aaccaccagc gggtccagct
     4561 gacggctggt ggagtccagc gggtcaacgg ccgggtagat acccagagac gcgatctgac
     4621 ggctcagtac cacggttgcg tcaaggtgcg caaaggtggt tgccggagac gggtcagtca
     4681 agtcatccgc aggtacgtat actgcctgta cggaggtgat agaaccagtt ttggtggagg
     4741 tgatacgttc ctgcagaacg cccatctctt ccgccagggt cggctgataa cctaccgctg
     4801 aaggcatacg gcccagcagt gcggatactt ccgtacctgc cagggtgtaa cgatagatgt
     4861 tgtcaacgaa cagcagaacg tcacgacctt cgtcacggaa tttctcagcc atggtcagac
     4921 cggtcagagc aacgcgcaga cggtttcccg gcggctcgtt catctggcca tacaccaggg
     4981 atactttgtc gataacgttg gagtcggtca tttcgtggta gaagtcgtta ccctcacgag
     5041 tacgttcacc tacgcccgca aacacagagt aaccggagtg ctcgatcgcg atgttacgaa
     5101 tgagctccat catgtttacg gttttaccta cacccgcacc accgaacaga ccaactttac
     5161 cgcccttagc gaacggacac atcaggtcga taactttgat accggtttcc agcagttcct
     5221 gagagtttga cagctcttcg taggaaggtg ctgcgcggtg aatcgcccaa cgctcttctt
     5281 caccgatctc gcctttcatg tcgaccggtt cacccagtac gttcatgata cggcccagag
     5341 tcgctttacc taccgggact tcgatcgggt gttcgaggtc ttttacatcc agaccgcgac
     5401 gcagaccgtc ggaggaaccc attgcgatgg tacgcacgat accgccgccg agctgctgct
     5461 gaacttccag caccagacgc tcattaccat tttgcacctc aagagcatcg tacacgcgcg
     5521 gtacggcatc ctgagggaat tcgacgtcaa ctacggcgcc gattacctgg acaatctttc
     5581 cagtagccat cttaaatcct ctacgaaata acctgtttaa accgcggcgg cccccgagac
     5641 gatctcggtg agttcctgag taatgctggc ctgacgagct ttgttgtata ccaactgcag
     5701 ctctttaatc aggctgccgc cattgtcggt cgcggctttc atcgccacca tacgggcggc
     5761 ctgctcgctg gccaggtttt caaccacgcc ctgataaacc tgagattcga cataacgacg
     5821 cagcagggta tccagcaacg ccttcggatc gggttcgtac aggtaatccc aggatttatg
     5881 tttcagatca tcatcatctg atgccggtaa cggcagcagc tggctgatgg tcggaacctg
     5941 agacatggtg ttaataaatt tgttgctgac aatgtaaagc ttgtccagac ggccttcgtc
     6001 gtaggcctgc aacatcactt ttaccggacc gatcagttcg gacagggaag ggttatcccc
     6061 catgccggtg acctgggcaa caacattgcc gcccacggag ttgaagaacg acacgccttt
     6121 cgagccgatc attgcgaggt cgcattgaac gcctttgtcg gtccaggtct tcatttccgc
     6181 cagcagtttt ttgaacaggt taatgttcaa accaccgcac aaaccacggt cggtcgacac
     6241 caccaggtag cccacgcgtt taacgtcgcg ttcttccagg tatgggtgct tatattccag
     6301 attaccgctc gcaaggtgac caatcacttt gcgcatggta tctgcataag gacggctggc
     6361 cgccatgcga tcctgcgatt tacgcatttt ggaagcggcg accatctcca tcgctttagt
     6421 gatcttttgc gtgttctgga cgcttgcgat cttactacgt atctcttttg cgccggccat
     6481 gagcttctcc tcaatgcctt gcggcctgcc ctaaggcaag ccgccagacg ttaccaggat
     6541 tgggttgctt tgaaggaatc gaggatgcct ttcagcttgc cttcgatttc gtcgttgtag
     6601 ccaccggtct ggttgatctc ttgcatcaac ggagcgtgat cacggtcgac gtaagccagc
     6661 agagcggctt cgaagctgcc gattttcgac agttcaacat ccgccaggta accacgttct
     6721 gctgcgaaca gaaccagaga ctgttgcgct acggacatcg gcgcatactg tttctgtttc
     6781 agcagttcgg tcactttctg accgtggtca agctgcttac gtgttgcatc gtcaaggtcg
     6841 gatgcaaact gagagaacgc tgccagttca cgatactgtg ccagagcggt acggatacca
     6901 ccggacagtt ttttcatgat cttggtctgt gctgcaccac caacacggga tacggaaata
     6961 cccgggttaa ccgcaggacg aataccggcg ttgaacaggt tggtttccag gaagatctga
     7021 ccatcggtaa tggagattac gttggtcgga acgaacgcag aaacgtcacc cgcctgagtt
     7081 tcaataatcg gcagtgcggt cagagaaccg gttttccctt tcacttcacc tttggtgaag
     7141 gcttcaacgt attcggcgtt aacacgcgca gcacgctcca gcagacgaga gtggaggtag
     7201 aatacgtcgc ccgggaatgc ttcacgtcct ggcggacgac ggagcagcag ggagatctga
     7261 cggtaagcaa cagcctgttt agacaggtca tcgtaaatga tcagcgcatc ttcaccgcgg
     7321 tcacggaagt attcgcccat tgcgcaaccg gcatacggag ccagatattg cagtgcagcg
     7381 gattcagacg cggttgctac cacaacgatg gtgttagcca gtgcgccgtg ctcttccagt
     7441 ttacgtacca cgttagaaat ggtggacgct ttctggccga tagcgacata gatacatttg
     7501 ataccggaat cgcgctggtt gatgatggca tcgatagcca gtgcggtttt accggtctga
     7561 cggtcaccga tgatcaattc acgctgacca cgaccaattg ggatcatgga gtcaacggct
     7621 ttataaccgg tctgtaccgg ctgatctacg gactgacgtt cgataacgcc cggagcgatt
     7681 gcttctacag cagagaagcc gtcgtgatcc agcggacctt taccgtcgat tggtgcaccc
     7741 agagtgttaa ccacacggcc cagcaggcca cggccaaccg gaacttccag gatacggcca
     7801 gtacacttaa ctttcatgcc ttcggcaagg tcagcgtacg gacccataac aaccgcacct
     7861 acagagtcgc gctcgaggtt cagtgcgata gcgtaacggt tacccggcag ggagatcatt
     7921 tcaccctgca tacaatcggc caggccgtga atgcggataa caccgtcact tacagaaaca
     7981 atagtacctt cgttgtgagc ttcactcaca acattgaact gagcaatgcg ctgcttgatc
     8041 agttcgctga tttcggtgga attcagttgc atgctccagt ccccttaaga ctgcaagacg
     8101 tctgcaaggc gctcaagacg accgcgtacg ctgccatcaa tgaccatatc acccgctcgg
     8161 atgataacgc ctgccattac agacttatcg attttgcaat tcagcttaac tttgcgtgac
     8221 agacgttttt ccatcgcagc agaaattttc gcgagctgtt gttcactcag tgcggcagcg
     8281 gaaatgacgt ctacctcagc ggtagcctca ctcacggcac gaaggtgaat aaactgctcc
     8341 agaacatccg ggagcgcgtt aagacgacca ttttcagcca ttacccgaat caggttctga
     8401 ccgttttcgt ccagttgctc accacaaact gcgataaacg actcggcgag cgtttctggc
     8461 gcaagcgcgc cagagagaag ctctgccatt tgttcgtttt tggttacctc ggcggcaaac
     8521 gccagcatgt cctgccagcg ttctacactt tggtgttcga cggcaaagtc aaaagctgct
     8581 ttggcgtagg ggcgagctac cgtaataaat tcagacatca gcccctccct ccttacagtt
     8641 cagcgacaag tttatccacg atgtcgctgt tagcagcttc atccacggaa cgttcgatga
     8701 tcttctcggc gccagcaaca gccaggatag caacttgctt acgcagctct tcacgggcac
     8761 gtttacgctc ggcttcaatt tctgcctgcg cctgggccac gattttagta cgttcctgtt
     8821 ctgcctcagc tttcgcttcg tccagaatct gcgagcggcg tttgttcgcc tgctcgatga
     8881 ttacctgggc ttccgctttc gcttttttca gctggtcggt cgcgctggcc tttgcaaggt
     8941 caaggtcctt atgtgctcgt tctgcggaag caaggccgtc agcaatttct ttttgacgtt
     9001 tttcgatggc tgccattaat ggcggccata cgtacttcat gcagaacaga acgaacagga
     9061 caaacgcgat ggcctggccg aggattgttg cgttaagatt cacagcacaa tgcctctatt
     9121 tagttaacgt tctgatattg ctctttaaat aaaagcaacg cttactacgc gacagcgaac
     9181 atcacgtaca gacccagacc tacagcgatc atcgggatag catccaccag acccataacg
     9241 ataaagaact gagtacgcag cagaggaatc agatcaggtt gacgcgctgc gccttccagg
     9301 aatttacccc cgaggatgcc gataccgatc gcagcaccga ttgccgccag acccatcatc
     9361 acagcggcag ccatgtacag cagatccata ttcaggtttt ccatgacagt ctccagtttg
     9421 tttcagttaa aacgtagtag tgttggtaaa ttaatgttct tcagacgcca tcgacagata
     9481 gacgatcgtc agaaccatga agatgaaggc ttgcagcgta atgatcagga tgtggaaaat
     9541 ggcccacggc acattcagga tccactgtga ccaccacggc aacagaccag caatcagaat
     9601 gaaaatcagc tcaccggcat acatgttacc gaacagtcgc aaaccaagtg aaactggttt
     9661 ggacagcagg cttacccctt caaggattaa gttgacagga atgaacgccc agtgattgaa
     9721 cggctgcagc gtcaactctt tcgtgaagcc gtcgatgcct ttcattttga tgctgtagaa
     9781 cagaatcagg ataaatacgc ccaatgccat agacagcgtt acgttcacgt ccgcagacgg
     9841 aaccacacgc agtgcaggca gacccagtac atgttcagca atgtacggca gcaggtcgat
     9901 aggcagtaaa tccatcaggt tcatcaggaa tacccagacg aagatcgtca gggccagcgg
     9961 agcaatcagc ttgcttttgc catggtacat gtctttcacg ctaccattaa caaagccgat
    10021 caccagctca atcgcggtct gaaacttacc tggcacaccg ctggtcgcct ttttggctac
    10081 gctacggaat aaaaccagga acaacagacc cagcaccacc gagaagaaca tggagtcaat
    10141 attgattgtc cagaaggtgg ctggggggtt ttgtggatcc accagcgaga atgtacgcag
    10201 gtccagctga aggttattca ggtggtgtcc tatgtaatcc tgcggcgtca tattttctga
    10261 agccatgatg ccttttaccc tttgttgtta attacagccg gtgccagtat ctgaaccacc
    10321 agcaccaaaa cccacgtaac gatcagcggc aagaataccg cctttaaaac cgccaacgcc
    10381 accaccagta acaccaacat cgccagaact ttgaaagctt cgccaaatgc gaatgtccag
    10441 gccacccggc ctttcgctgg tgtatgcgcc tggtgacgcc aggcaaatat cataaacaaa
    10501 acgttaggca gaaagactgc caggcccccg cttattgcag agacgcccca gaaggggtct
    10561 ttgaggctga acagcaatcc acttgctatc accaccagta actgaacgag cagaagcttc
    10621 cgagcaacgt ttcgactcac gagcgacaca gacatcacgt ttttcactcc tgctcccttc
    10681 gaggtatgcc gcgtgtcgta taaaactttc tttaaggctt agagtcaagc atcaaaaagc
    10741 ggtcaaatta tacggtgcgc ccccgtgatt tcaaacaata agtagccaaa aggtgaataa
    10801 atgtttaaat atttttccag tgcatacaat tgcgactttt ctgctaaccc tgttcgatca
    10861 tgaaaaactg taaataacgc gtaaacactg gtgataaagc gtgcttcaga tcacatattg
    10921 cgcatgttcg cgcacagcat atttatttac ttggcaaatg atgcctttgc aagtttatga
    10981 tatttcagtc taaaaacaga ta
//
LOCUS       DAZGXZ010000159        11001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000159 DAZGXZ010000000
VERSION     DAZGXZ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.159"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(451..663)
                     /gene="yncH"
                     /locus_tag="ACHKJF_004485"
     CDS             complement(451..663)
                     /gene="yncH"
                     /locus_tag="ACHKJF_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFP0951734.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDTININTNKNIRQVC"
     gene            complement(739..1356)
                     /gene="yncG"
                     /locus_tag="ACHKJF_004486"
     CDS             complement(739..1356)
                     /gene="yncG"
                     /locus_tag="ACHKJF_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP0951735.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIAFMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            1622..3121
                     /gene="ansP"
                     /locus_tag="ACHKJF_004487"
     CDS             1622..3121
                     /gene="ansP"
                     /locus_tag="ACHKJF_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP0951736.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3236..4297)
                     /gene="yncE"
                     /locus_tag="ACHKJF_004488"
     CDS             complement(3236..4297)
                     /gene="yncE"
                     /locus_tag="ACHKJF_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP0951737.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4539..6641
                     /gene="pqqU"
                     /locus_tag="ACHKJF_004489"
     CDS             4539..6641
                     /gene="pqqU"
                     /locus_tag="ACHKJF_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP0951738.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6677..7342)
                     /gene="mcbR"
                     /locus_tag="ACHKJF_004490"
     CDS             complement(6677..7342)
                     /gene="mcbR"
                     /locus_tag="ACHKJF_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP0951739.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(7540..8577)
                     /gene="curA"
                     /locus_tag="ACHKJF_004491"
     CDS             complement(7540..8577)
                     /gene="curA"
                     /locus_tag="ACHKJF_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP0951740.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8758..9276
                     /gene="mddA"
                     /locus_tag="ACHKJF_004492"
     CDS             8758..9276
                     /gene="mddA"
                     /locus_tag="ACHKJF_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP0951741.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            9273..9722
                     /gene="ydcZ"
                     /locus_tag="ACHKJF_004493"
     CDS             9273..9722
                     /gene="ydcZ"
                     /locus_tag="ACHKJF_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0951742.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(9723..9956)
                     /gene="ydcY"
                     /locus_tag="ACHKJF_004494"
     CDS             complement(9723..9956)
                     /gene="ydcY"
                     /locus_tag="ACHKJF_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP0951743.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(10042..10215)
                     /gene="ortT"
                     /locus_tag="ACHKJF_004495"
     CDS             complement(10042..10215)
                     /gene="ortT"
                     /locus_tag="ACHKJF_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP0951744.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            10410..10505
                     /gene="yncL"
                     /locus_tag="ACHKJF_004496"
     CDS             10410..10505
                     /gene="yncL"
                     /locus_tag="ACHKJF_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP0951745.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(10907..>11001)
                     /locus_tag="ACHKJF_004497"
     CDS             complement(10907..>11001)
                     /locus_tag="ACHKJF_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HFP0951746.1"
                     /translation="VEEMNRLISDGNIQERDYKKFNLSEVFRGL"
BASE COUNT         2736 a         2649 c         2593 g         3023 t
ORIGIN      
        1 ccccaccctg ataacatgat ctgcacattg ccatcttaac gtcgttaaac actggcttaa
       61 tagtggatat ttcaaattag gaatttatta aattcacctg atgaatatca taagcaaata
      121 gcatagaaaa taatgacaac tcccccgagg ggaccatttc caaagcactg caccaccacc
      181 tggtgccgga tgatcttgct tcagtggtaa gagtacctga tgcattgttc ccaaaatggt
      241 gttttcccct gcccctccaa taatgtgtag ctctcacttg ataatctatt gattaggtta
      301 tattaagaat caacatttaa aatacatcca ttttcttgaa taaatcaaca tgtttattaa
      361 tcaattgctg ttttagctct gactcatcaa gctctccata tttgtcctac agataattgc
      421 gaataaatac agagaacgcc atttatcctt ttagcatacc tgccggatat ttttatttgt
      481 attaatatta attgtatctc caactccgag catatccatc attaaaaact ttttgagatt
      541 atagaatacc tgtgataaca agtgaaacat ctgcacaatg tgaatagtta gtttcttttc
      601 agaggaaatg aaatagacaa gctgaatgaa ggggagagtg atataaatca aaaaacataa
      661 catgaattta attaattaca cccatcattg tggataaagt tactgaataa taaataacat
      721 aatcttctgg tatcccactc aaataatttc attccttttt aatacttctt gtaatttcgg
      781 taactgacat acagcatcag cgatcgcgct gatattctgc gcattatcct gaaaccattc
      841 atgccccggc ccccatgtgc gcatggtgca gagataacag tccaccagcg ttagttgctc
      901 accaaaggca taaggttcag cggtcaattg tgaattcagc cagatataaa gtgatttgcg
      961 atattcaatt acattcttct ttaactgttc aggtgcgtca gatgcccaac gctcagggta
     1021 atcggcgaaa gtgaatgttg gatagacatt agcaaccagc cagaccaata gccgttgaaa
     1081 ctgctgacgt tcggcgcgcc caaccggtgg cgcaaggtcc gggcgacgat cgagaaccat
     1141 aaatgcaatc gccgctgtct ccgtcataat ctcgtcattt tccagcgcca gtgtcggaac
     1201 ctgacacaac gggttcaggg ttttcaataa ctcgcgtgaa gctccctcgt ggtcaaaacc
     1261 actgacatca acgaactgat aaggaatatc agccagtgtt agcatcagct cactgattgt
     1321 agagccccat ccgggtacgc catacacctt aatcatatgc cctcacaatc ttttaaagat
     1381 taagtgtagg acagtacaca ggttaaccag caaaaagatt tgtcattttt tcgtcagttg
     1441 tattattccc cttcaggatt gtaaggggat gttttgcatc gtttatcgat aacgggtagg
     1501 atgctgcgcc gatagtttta tacccataaa gaataatggt gataactatc atcgccagga
     1561 tgaataaaca ttgttcatgg caacttatat gactttttca ttaaagcaat cagggagagc
     1621 aatgagtaaa cacgacaccg acacttcaga tcaacacgcc gcgaaacgcc gctggcttaa
     1681 tgcccacgaa gaggggtatc acaaagcgat gggcaatcgc caggtacaga tgatcgccat
     1741 tggcggcgcg attggcaccg gcttgttttt aggtgcagga gcccgactgc aaatggcggg
     1801 tccagcattg gcactggttt atttaatttg tggcttgttt tcgtttttta ttctgcgtgc
     1861 attgggtgag ctggtgctac accgcccttc cagtggcagt tttgtctctt atgcccgtga
     1921 gtttttgggt gagaaagccg cttatgttgc tggctggatg tacttcatca actgggcgat
     1981 gacggggatt gttgatatta ccgccgtcgc tctgtatatg cattactggg gtgcgtttgg
     2041 cggcgtgccg cagtgggtct ttgcgctcgc tgcacttacc atcgttggca ccatgaatat
     2101 gatcggtgtg aaatggtttg cggagatgga gttctggttt gcgcttatta aagtgctcgc
     2161 cattgtgacc tttttggtcg tgggtacagt gttcctcggt agtggtcagc cgctggatgg
     2221 caacaccact ggctttcatt taattaccga taatggcggc ttcttccccc acggtttgct
     2281 gcctgctctg gtgttgattc agggcgtagt gtttgctttt gcctccattg aaatggtggg
     2341 tacagctgcc ggagaatgta aagatccgca gaccatggtg cctaaagcga ttaacagcgt
     2401 gatttggcgt attggcctgt tttacgtcgg ctccgtggtg ttgctggtta tgctattgcc
     2461 gtggagcgcg tatcaggcgg ggcaaagtcc gttcgtgacg tttttctcta aactgggtgt
     2521 gccatatatc ggcagcatta tgaacattgt ggtgctgacc gctgccctct ccagcctgaa
     2581 ctcaggtctg tactgcaccg gacgtattct gcgctcaatg gcgatgggcg gttccgcacc
     2641 gagttttatg gcgaaaatga gtcgtcagca tgtgccgtat gccgggattc tggcgacact
     2701 agttgtgtat gtcgtcggcg tattcctcaa ctatctggtg ccgtcgcgcg tatttgagat
     2761 tgtgttgaac ttcgcgtcgc tgggaatcat cgcttcatgg gcgtttatca tcgtgtgcca
     2821 gatgcgcctg cgtaaagcaa ttaaacaagg caaagcagcg gatgtcagtt ttaaactgcc
     2881 tggtgcgccc ttcacttcct ggctgacatt actgttttta ctgagtgtcc tggtgctgat
     2941 ggcgttcgat tacccgaacg ggacttatac tatcgcggcg ctgccgatta tcggtattct
     3001 gctggttata ggctggtttg gtgtgcgcaa acgcgttgct gaaattcaca gcactgcgcc
     3061 agtcgtcgag gaagatgaag aaaaacagga aattgtgttt aagcctgaaa cggcgagcta
     3121 attgcaaaat tgcccggcta cgtgccgggc aaatttagat tactgacgaa ggtgtgccgc
     3181 atgcggcgtg ataaaagtca ggcaagtctc tccgttttca ccctcgccac atctattaca
     3241 gcgcaatacg aatcacatcg tccggctggg tagcttcctg ctgtttagtg gatttttgtt
     3301 tcacactgac atacagcgtt ttgccatcgg cagacagcgc caggctgttt ggatgagtcg
     3361 gcgtatcgaa cgttttcacc actttatagc ttttcgcgtc aatcacactg actttacctg
     3421 cctgacgatg cgttacgtag gcttcattac gcgctgggtt aaacagcaca gccagtgatt
     3481 ctggtgccgc aaccttcgcc agaatattgc cattacgggt atcgaccact aacacttctg
     3541 cggctttaga atcggtgata aatgcacgct gcctggcggt atcaaggcta atgttgataa
     3601 agaagtgctc tttgccgtca tccagcagct ttttacggct gaggatttta ttgtcggcgg
     3661 tgtcgatggt aatcaattcg ccgtcagcgt tagtggtgta aagacgtttg cctttgctat
     3721 ccagcgccag accggtactc attttaccgg tgttctggat ggcggttttc agtttgatat
     3781 tctcgccatc aacgacccaa atcacgctct ctttaccaat accactgatg taaacggtgt
     3841 tcgtggcatc atcagctacc agctcacgcg gttgcagcgg gcgcacctct tccgtgcgct
     3901 tacgatcatc cagcaccaga cggcctttca cctcgcccgt tttggcatct atcgccgtga
     3961 ccgcgctgtt tacggtgtta ccaaaccaca acgtctgagt cgtgttattg atggtggcac
     4021 caaacggctt gagatcgtta tggatcgcct gcgtcacttc cagagtgacc ggatcaagac
     4081 gataaaccac gccaccttta tccagtttgc ggctttgcga agtggcgagc cacagtgcgt
     4141 tttcttgctg gctataagcc atttcgtagg cacctttacc taccgcttta cgcagcattt
     4201 cttctgcggc ctgcgtactg aatgatgaag caacaagcaa tgaacctaac agtaatgaac
     4261 cacgcaggcg cgatgaaaac agatgacgta aatgcatgac gactcccttt gatgaaccga
     4321 tatttttgct gcttcacaat gcttcgctaa gcgtaatcat cgttacgctc ttgttataaa
     4381 tgggaatggt aatcattatt ttctcaaatg tggagcattt cttgccgtta cgactacaat
     4441 tttctcaatt acttaacgta tcgtgccgtt tcgttttcca taaatttaca atttcattac
     4501 cataacggcg ttttcccatt cttaacctgg cttttttcat gaagattttt tccgtccgac
     4561 agaccgtttt gcccgcactg cttgtccttt cccccgttgt ttttgccgct gatgaacaga
     4621 ctatgattgt cagtgccgca ccgcaggtgg tttcagaact ggatacccca gcagcagtaa
     4681 gcgtggtgga tggcgaggag atgcgcctgg caacaccgcg cattaacttg tccgaatcac
     4741 tgaccggcgt gcctggtttg caggtacaaa accggcagaa ctatgcgcaa gatttacagc
     4801 tgtcgattcg cggatttggc tcccgctcca cttacggtat tcgcggtatt cgcctgtatg
     4861 tggacggtat tcccgccacc atgcccgacg ggcaagggca aacatccaac atcgatttaa
     4921 gcagtgtgca aaatgtggaa gtgctgcgtg gccccttctc tgccctgtat ggcaacgcgt
     4981 ctggtggggt aatgaatgtc accacccaga ccggacaaca gccaccaacc attgaagcca
     5041 gtagttacta cggcagtttt ggcagctggc gctatgggct gaaagcaacg ggcgcaacgg
     5101 gagacggcac acagcctggc gatgtcgatt acaccgtctc aaccacgcgt tttacgaccc
     5161 acggctatcg tgaccatagt ggcgcacaga aaaatttagc taatgccaaa ctgggcgtac
     5221 gcattgatga agccagcaaa ttaagtctga ttttcaatag tgtggatatc aaagcagatg
     5281 acccaggtgg gctaaccaaa gcagaatgga aggctaatcc acaacaagcg cctcgtgcag
     5341 aacagtacga cacgcgaaaa accatcaagc aaactcaggc tgggttgcgc tatgagcgta
     5401 gcctgagcgc gcaagatgat atgagtgtga tgatgtatgc cggagagcga gaaacgaccc
     5461 agtaccagtc aatcccgatg gccccccaac ttaacccgtc acatgcgggc ggcgtgatta
     5521 ccctgcaacg ccattaccag gggatagaca gccgctggac acaccgtggt gaactgggcg
     5581 ttccggtcac gttcactacc ggcctgaact acgaaaacat gagtgaaaac cgcaagggct
     5641 acaataactt ccgcctgaat agcggcatgc cggagtacgg gcaaaaaggt gagttgcgtc
     5701 gcgacgaacg caatctgatg tggaacatcg atccctattt acagacgcag tggcagctga
     5761 gcgaaaaact gtcgctggat gctggcgtgc gctacagctc cgtgtggttt gattccaacg
     5821 accattacgt tactccgggt aacggcgatg acagcggtga tgccagttat cataaatggc
     5881 tacctgccgg ttcgttaaaa tatgcaatga ccgatgcctg gaatatctat ctggcagccg
     5941 ggcgaggttt tgaaacgccg acgattaatg agctgtctta tcgtgctgat gggcaaagcg
     6001 gtatgaactt tggtttaaaa ccatccacca acgatacaat tgagatcggc agtaaaacgc
     6061 gtattggtga tgggctgctt agtctcgcat tgtttcagac cgacactgat gatgaaattg
     6121 ttgtcgatag cagtagcggt gggcgtacga cttacaaaaa tgccggaaag acccgtcgtc
     6181 aaggcgctga actggcatgg gatcaacgtt tcgcaggaga ttttcgcgta aacgcgtcct
     6241 ggacctggct tgatgcgacc tatcgcagca atgtttgcaa tgaacaggat tgtaacggta
     6301 atcggatgcc agggatcgcc cgtaatatgg gctttgcgtc gataggttat gtaccggaag
     6361 atggttggta tgcaggcacg gaagcgcgtt atatgggcga tattatggca gatgatgaaa
     6421 atacggcaaa agcgccgtct tatactctcg tcggcttatt caccgggtat aaatacaatt
     6481 accacaattt aactgtggat ttatttggtc gtgtcgataa tttattcgat aaagaatacg
     6541 ttggttctgt cattgtcaat gagtcaaacg ggcgatatta cgaaccttcg cccggacgaa
     6601 attatggtgt cggcatgaat attgcgtgga gatttgagta attaccttga tgcccggtat
     6661 ttgccgggca tttactttaa cgattgtatt gctggtataa aatagcaata ttttgttgca
     6721 taatttcagc aagacaatgt ctgcatccct ctttgtcttt tgcttttaac gcagcgagta
     6781 acagatgata gttttctata tgctcccgta attccgctgg attaatcgct tcatagagat
     6841 aatgtaaacc aggccccatc ctgacccaca gttgctcaat catctcacac aggatgggca
     6901 tatttgagcg atgataaatc gctaagcgaa atagcctgtt tacattaatg atttgttcca
     6961 tgtcgccctt ttcttgcgcc tgctgtaatt tctcgagcaa ttcctggagt tccgccaggt
     7021 cttgcggggt gaggttttca acagccagag caactgccat taattccagt tcgtagcgga
     7081 tccgattgat ttcgtctaat tgacgtttcc ccacttccgg aactgtaaat gcttgtgcag
     7141 gtgcgacaga aagcgcattc accgaaacca gacgtaataa tgcttcacgc acaggtgtaa
     7201 tactcatacc taattgctcc gccagatttt tagtaatcag gcgtgcgcca ggttttaatg
     7261 cgccaatact aagctgatgt ttcaggtcgt tctcaacctg cagagtcaaa ctgacatgtt
     7321 tcatttttcc cattccaggc attttaattc cttcgcgtgt tttctgtcca accctgcctg
     7381 cacggcagaa ggagttcacc tggtgtatta aagtgaataa tcataaacca cccacctttg
     7441 ttgttattag cagcagaaca atgacgtggt tagttaatta aagctgaaaa taagcttttt
     7501 gcctggattt ttatggcggc ttacgccgcc gttgaaactt taatcatcac ccgcgacgcg
     7561 gatcaccact ttgccgaagt ttttaccctt cagcaggccg ataaacgtct gtggcgcatt
     7621 ctctaaacca tcagtaatat cttcgtggta gtggatttta tcctctttca cccattgccc
     7681 catctccttc tgaaactcat ggatgcggtg accataatcc tgagcgataa taaacccttg
     7741 caagcgaata cgttttttca gcaccgttgc catcaacagg ggtaaacgat ccggaccggg
     7801 tggtagctct gtagcgttat agctgctcac taagccacaa acgggaatgc gcgcagatgt
     7861 attaagtaat ggtagcactg catcaaatac cttaccgccc acgttttcat aatagatatc
     7921 aatacctttt gggcacgctt tcaccagttg ttcggcaaaa tcatccgcgt ggtgatcaag
     7981 acaaacatcg aagcccagca cctcgatagc atggcggcat ttttccgcgc caccggcaac
     8041 ccccaccacc ctgcaacctt taagtttgcc gatttgcccc accgttgctc ccacaggacc
     8101 tgtcgccgca gctaccacca acgtttcgcc ctctttaggc tgaccgatat ccagcagccc
     8161 catataagcg gtaaagcctg gcatccccag cacacccagc gaccacgatg gattttgcgg
     8221 atgatcgcca agtttcacca gatcatcacc actggatatg tcatagtcct gccatccact
     8281 gtagcccagc acccagtcgc caggctgata atcaggatga ttcgactcca caacacggct
     8341 caccgtgccg ccgaccatca cgccgccaat atcaacaggc ggtgaataag atggctcatc
     8401 gctcatacgt ccacgcatat acgggtccag ggacaaataa actgtgcgca gtaacacctg
     8461 tccttcaccc ggtgtggcga catcatcttc ttcaagacgg aaattctccg gaaccggtgc
     8521 gccatgtgga cgcgaggcca gaacccaacg tcgattacgc tgcttttgtt gccccataat
     8581 gctctcctgt gcttttggca tgaatccatc aatagtagca ggctcacggg attggttttg
     8641 tttgcagccg tcacatcccc tctgcaacac gcaataattt catcgaggaa tccgttgctt
     8701 cgggctgttt tcgatagtat tgataacatc atcatcatta ttttatcgag gccagacatg
     8761 tccatccgtt ttgcccgcaa agccgattgt gctgccattg cggaaattta taaccacgcc
     8821 gtgttgtata cggcggctat ctggaatgac caaacggttg atgcagataa ccgcattgcc
     8881 tggtttgaag cgcggaccat agcaggttat ccggtgctgg taagtgaaga agatggcgta
     8941 gtgacgggat atgcctcgtt tggcgactgg cgtagtttcg atggttttcg ccataccgtg
     9001 gaacattcgg tttatgtcca tcccgatcat cagggcaaag gtctggggcg taaattgtta
     9061 agccgattga ttgatgaagc gcgggatagc gggaagcatg tcatggtcgc cgggatcgaa
     9121 tcgcaaaatc aggcctcgct gcatctgcac cattcgctgg gatttgtcgt caccgcacaa
     9181 atgccgcagg tgggcactaa atttgggcgc tggctggatc tgacatttat gcagttgcaa
     9241 ctcgacgaac gcactgaacc ggacgcgatt ggatgaatca gtcgctcacc cttgcttttt
     9301 tgattgccgc cggaattggt ctggtagtcc agaacacgct gatggtgcgc atcacccaga
     9361 cctcctctac cattctcatc gccatgttgc tgaactcact ggtggggatt gtgctctttg
     9421 tttctatttt atggtttaaa caaggcatgg cggggtttgg cgaactggtg tccagcgtgc
     9481 gctggtggac acttataccc ggcttgctgg gatcgttttt tgtcttcgcc agtatcagcg
     9541 gatatcagaa tgtgggagcg gcgacaacta ttgcagtact ggtcgccagc cagctaattg
     9601 gcggtctgat gctggatatc ttccgcagtc atggcgtacc actacgggcg ttgttcggcc
     9661 caatttgtgg cgcaatttta cttgtggttg gcgcctggtt ggtagccaga cgctcatttt
     9721 aatcagagga tggtgccgcc tttggttaac tgttcgtggc gcgcttccat atcttcttta
     9781 tgcttgcgac cgtgatgagc aatagcggtg cgcaaacgct gctgctgggt gtagcgatct
     9841 tcccgactta actccgcgtc atcgctcagg gcgattaata attcgttcat atgggcaatg
     9901 acgctctctt caatcgcggc atccacgcgc gcgatgactt catccagatg cgacatactt
     9961 tctccttaac cacttgtgaa atttatcgat tcatccaaat gatataagtc tgcgtggtgc
    10021 cgtcaagcca gtgagtaaga tttaaaacag tgaaaacaac agcgccaccg ggaaactcat
    10081 cggccatgtt gcccccacca gaaaggcgct taagaaacgg atgcgtttct tatcgtgaga
    10141 aagaaaccag gtgataagaa atgcgattgc tgccataacc gcataaaaaa caagcatatg
    10201 ttgatagaga gacatgtacg ggcgtccagc cagatgaata aaacgcgcgc aaatatgcgt
    10261 cattttttga cgtgcatcaa gttttttcac cagtttgaga aaaaatcatc aaacagcaac
    10321 ttgcccttat ccatcttcag aatgagtatt attgcggatt ttcttaaccc gtactataca
    10381 cataggggca atgataaaag gtggcaaaaa tgaatgtttc cagtagaact gtagtactga
    10441 taaatgtctt tgctgctgtt ggtttgttta ctcttatctc tatgagattt ggctggttta
    10501 tttgatgtag tgattttgca cgcagcacgt actgtcagag aaatcaactc tctgacagta
    10561 cgcaagatct tctcatactc tccaggttta caacattatc cttaatattt acttcttttt
    10621 gctggcatct acattgaaat gagtagaaaa catcccccta ctgtcgtcta tacaaacagc
    10681 ttactgattc aaacggaatc ttgttttcac atttttctat catgaatact ttatacgcgc
    10741 gggagtctat aaatgcttta tactttcaag gaataacgta aatataaaaa gatggcgtaa
    10801 agtatatgca tggcaggatg tttgcaacct ccttgatgac gctgccccat tgaaattgca
    10861 aaccccgccc tggttatctt gcccatttat catgcaaaac agataattat aggcctctga
    10921 atacctcaga aagattgaat tttttatagt ccctctcttg aatatttcca tctgatatca
    10981 atcgattcat ctcttcaaca a
//
LOCUS       DAZGXZ010000160        11000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000160 DAZGXZ010000000
VERSION     DAZGXZ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.160"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            125..886
                     /gene="adiY"
                     /locus_tag="ACHKJF_004498"
     CDS             125..886
                     /gene="adiY"
                     /locus_tag="ACHKJF_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP0951747.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            1023..2360
                     /gene="adiC"
                     /locus_tag="ACHKJF_004499"
     CDS             1023..2360
                     /gene="adiC"
                     /locus_tag="ACHKJF_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP0951748.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDVPISKD"
     gene            2464..4107
                     /gene="eptA"
                     /locus_tag="ACHKJF_004500"
     CDS             2464..4107
                     /gene="eptA"
                     /locus_tag="ACHKJF_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP0951749.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4104..4772
                     /gene="basR"
                     /locus_tag="ACHKJF_004501"
     CDS             4104..4772
                     /gene="basR"
                     /locus_tag="ACHKJF_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP0951750.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4773..5873
                     /gene="pmrB"
                     /locus_tag="ACHKJF_004502"
     CDS             4773..5873
                     /gene="pmrB"
                     /locus_tag="ACHKJF_004502"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP0951751.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5849..5938)
                     /gene="pmrR"
                     /locus_tag="ACHKJF_004503"
     CDS             complement(5849..5938)
                     /gene="pmrR"
                     /locus_tag="ACHKJF_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP0951752.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(6050..7552)
                     /gene="proP"
                     /locus_tag="ACHKJF_004504"
     CDS             complement(6050..7552)
                     /gene="proP"
                     /locus_tag="ACHKJF_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP0951753.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7816..8694)
                     /gene="rdcB"
                     /locus_tag="ACHKJF_004505"
     CDS             complement(7816..8694)
                     /gene="rdcB"
                     /locus_tag="ACHKJF_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP0951754.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8691..10919)
                     /gene="crfC"
                     /locus_tag="ACHKJF_004506"
     CDS             complement(8691..10919)
                     /gene="crfC"
                     /locus_tag="ACHKJF_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP0951755.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHDDLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQARGYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2751 a         2860 c         2701 g         2688 t
ORIGIN      
        1 taatattagt aaaactcgtt atttttatgc atgtttatat tcatcataca attatataac
       61 catttcccgg tatcgctttg ctttagcgag aactggtctt ttatttgcac tcaggagtac
      121 atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa aagatgtctg
      181 gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg cgttattaaa
      241 ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg ctcatattag
      301 tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc aaataccagt
      361 atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag acgtctttcg
      421 cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg aacgaacacg
      481 cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc tttcattaat
      541 gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg aaagcgatat
      601 tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc caagtctgtt
      661 aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca cctgccgcat
      721 gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg tatcacagtc
      781 ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct acggtatgac
      841 gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt taaccttaac
      901 gaagagctat attaataacg gcatcagcga taacccggtc gataataatt caactatcga
      961 atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc aggagtttaa
     1021 ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc ctgatggtgt
     1081 cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc tctactggcg
     1141 ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc tcgatggtat
     1201 acgccaaaat gtcgttcctc gatccaagtc ctggtggttc ttacgcgtac gcccgccgct
     1261 gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc tgctggatcg
     1321 gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc ccgattctga
     1381 aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc gtcctgctga
     1441 acattgtcgg tccgaaaatg atcacccgtg tgcaggccgt tgccaccgtg ctggcgctga
     1501 tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa acctatatgg
     1561 cggcatggaa cgtcagcggc ctgggcacct tcggtgcaat tcaaagtacc cttaacgtta
     1621 cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg gtgaaaaacc
     1681 cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc gtttgctatg
     1741 tactttctac caccgcgatt atggggatga tccctaatgc cgcactgcgc gtttctgctt
     1801 cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgccggggcc attgtttcct
     1861 tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg gcgggtcaaa
     1921 cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt gtaaataaag
     1981 cgggcacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc ttccagctca
     2041 gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc tcggtcatct
     2101 ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggt cacggtcact
     2161 ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc tactgcattt
     2221 gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc ctgatggtca
     2281 tcaccgccat gtatgcgctg aattacaacc ggctgcataa aaacccgtat cccttagatg
     2341 taccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca accttaagaa
     2401 cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga tggaaacacc
     2461 gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg gctattgttg
     2521 gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt gttgcaggcg
     2581 ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt cgtcgctttc
     2641 agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg accactggcc
     2701 tgcctgttta ttctggttgg cgcggctgca caatatttca taatgactta cggcatcgtc
     2761 atcgaccgct cgatgattgc caatattatt gataccactc cggcagaaag ttatgcgctg
     2821 atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctgc gctgattgcc
     2881 tgctggataa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt ccgtggagcc
     2941 aatattctgg tttctgtact actgattttg ctggtcgccg cactgtttta taaagactac
     3001 gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc taacagcatt
     3061 gttgccagct ggtcatggta ctcccatcag cgactagcaa atctaccgct ggtgcgaatt
     3121 ggtgaagacg cccaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa tttgaccatc
     3181 ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta cccgcgtgaa
     3241 actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc atcttgcggc
     3301 acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga gcattacaaa
     3361 gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc gggcatcaac
     3421 gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt gcctcaccag
     3481 aacgtcaccg cgctgaatct gcctggtcag tgcatcaacg gcgaatgcta tgacgaagtg
     3541 ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt gattgtctta
     3601 cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca gttcaggaaa
     3661 tttaccccaa cctgcgacac caatgagatc cagacctgta gcaaagagca actggtgaac
     3721 acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat taatctgctg
     3781 aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca cggtgaatcg
     3841 ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc ggatagccaa
     3901 aaacaggtgc cgatgctgct gtggctgtcg gaggattatc aaaaacggta tcaggttgac
     3961 cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa tttattctcc
     4021 acgctattgg gattaactgg cgttgagacg aagtattacc aggctgcgga tgatattctg
     4081 caaacttgca ggagagtgag tgaatgaaaa ttctgattgt tgaagacgat acgctgttat
     4141 tgcagggatt gattctggct gcgcaaactg aaggctacgc gtgcgatggc gtgacaaccg
     4201 cgcggatggc ggaacaaagc cttgaggccg gtcattacag cctggtggta ctggatttag
     4261 ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa aaatacaccc
     4321 tgccagtact gatcctcacc gctcgcgata cgctgaccga caaaatcgct gggctggatg
     4381 tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac gcccgtatcc
     4441 gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt ggcaatctga
     4501 cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt ctgacgccca
     4561 aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggtg catcgggaaa
     4621 ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc ctggaagtgc
     4681 atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg cgcggctttg
     4741 gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcatttt ctgcgccgac
     4801 caatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg gtgtttgagc
     4861 tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg tttgagcagg
     4921 cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc gaggcggtcg
     4981 ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc tgctatcagg
     5041 cggtacgccg catcactcgc ccgctggcgg agctgcaaaa agagctggaa gcgcgcaccg
     5101 ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa gcggtggttt
     5161 cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa aggttgttta
     5221 ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg catctggaac
     5281 tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt gatcagatga
     5341 tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt tcttccggta
     5401 attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac gaactcagta
     5461 ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc gacatcaccg
     5521 ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa aacgcccatc
     5581 gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc ggagcggtca
     5641 tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag ttgagtaaag
     5701 cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt attgtcagcc
     5761 gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag acttccggca
     5821 cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata taaagaaagc
     5881 tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg tttttcattg
     5941 cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg aaccttaaga
     6001 gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct tattcatcaa
     6061 ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca atctcgtggt
     6121 caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt tccttcgctt
     6181 cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc gtctctttca
     6241 tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag gcaggcatca
     6301 tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga ccggcaacca
     6361 gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc gggaacatcg
     6421 ctggcaaggt agaagccata acgcccgtaa agcagttaag gatcaccgcc agcatcagta
     6481 acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc gggatcgcca
     6541 acacaaacag ggcaacacta ccaagtagca caaacggacg acggccaaaa cggtcactca
     6601 gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata ataatcagca
     6661 ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata taggtcagca
     6721 acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac aggctgcgcc
     6781 agtgtttggt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa ccttcgcggt
     6841 cgccctgttc cagtttatcg acatgctgct ggaacgccgg agtttcttcc agcgcatggc
     6901 gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata cgccagcccc
     6961 agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccactacg ccagcaccca
     7021 gcacaaaccc ggcaatagaa ccaaagtcca gccagctgcc cataaagcca cgtttacggt
     7081 caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccgccg accgagaaac
     7141 cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc gtgtcgtagg
     7201 acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg atagcgagga
     7261 tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca agcggtcgaa
     7321 tcagaaaggg aacggagaac gtggcaagtg cagcaaccat ctgcacgctg gggtcagccc
     7381 ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca aaatcaaacc
     7441 attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta ccgtcatcaa
     7501 taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc atagctttcc
     7561 tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc cgcaaaaggg
     7621 gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct tacaccctcc
     7681 ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa tgaccttttt
     7741 accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt aggactcatt
     7801 gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca ttgcccccgc
     7861 cagacgctgc gctgaacaga gccgtggcat ccccagcgcg acagtttgcc agcgttgggt
     7921 cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc aacttaaaaa
     7981 atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat ggtaatagtg
     8041 acgcgtggtc ggggccgcat tgatactctc tgccagatgt tgtgtcgctt tcacgcgcaa
     8101 atcacccagc aacaacgcca ccgcttgcgg gcttcccaga cgacaggcat cgccaaatgc
     8161 cccccaacgc aactcttcga gaacgataaa acagcgccca ggcaacgacc aggcgttata
     8221 cgccccagca cgccagcggg taaaaatttg ctcgctctgt tcacccgcct gcaccagtag
     8281 gcctcgttgc gttaatgcct tttccttcca gcgacaacgg gctgaaaacc actcatacaa
     8341 ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat ccattttttg
     8401 cagcagcgcc agatgctgcg cgatctgcgt cagtgccaga tgaccgggat ccagcatcga
     8461 cgccagtttt tccaccaatg tcagacgcag gctaagcggc gcatttaaca tgccgccgtt
     8521 cgtccatgtc tgaagctgca cgcgcgcaag aagttcctgc atcagtcgtt cacgaaactg
     8581 ctgctgatgg gcttgtggaa ggcgggcatc atcgccctgc gccagatcca ctaaaaagcg
     8641 gggataaacc gactccagca cgcgaccggg gccgtcaagt aacgtcttgg tcaatatcgt
     8701 tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gggcgtcggt gtaaatccag
     8761 ccgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac cgattcattt
     8821 tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attccgtcaa acatgaagcg
     8881 aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg ctctgccaga
     8941 actttagcca tttgttggca ataaagatcg acttctgcgc ttaaagctcg ttgcaacaca
     9001 ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc gggctgattc
     9061 aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc gtccgttgct
     9121 tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg aactacgccg
     9181 tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc cattttgact
     9241 tgttgctcaa tgggccgaat aacatcggtt aacacccggc aaagggtgga ttcaagctcg
     9301 gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa cagtgtctca
     9361 cagcaggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc ggctaactgc
     9421 gcattcagcg catcttgctg ttgacgcaga aagtggttgg cggaggtcag ggccagctcg
     9481 atttcatgtt taacctcgcc gctcacctgc gcctgattga gttgcaatag ctgcaaactt
     9541 tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa cccgtgcgca
     9601 cgaaaatcca ggtatccccg cgcctgctgc gcgtaattca acagtttatg cgcagcagat
     9661 cgcagagcat acaacgaggc gttagcgtaa gcggcatgaa gcaacgcctg aattggctgg
     9721 gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc gaggtccgcc
     9781 agatcgtcat gacgccagcg cctgccgagc gcagcatggg caaaatcttc cacccagcgt
     9841 tgttgctctg gcgctggtaa cttaccgttg ttggctaact catggcgcgc tcgattcgcc
     9901 aggtagcccc acatcgacga caccggaaat atctgctgtg gcgtaataca gcctttcatc
     9961 agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg atcctgttga
    10021 tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa aatcgcctca
    10081 cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc cagtaccgcc
    10141 gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc ggcttcattt
    10201 ggcccggggg tatccagtaa cgtcaactga ccgggataac tctccagccc cgccagatgg
    10261 acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga aaaaggaaaa
    10321 tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag acaatgaaaa
    10381 ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat ccgctgcata
    10441 agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat atcgcaatca
    10501 cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg tgaaaaatgc
    10561 agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc agtcattggg
    10621 cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt tgatttccct
    10681 gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt acgcaactca
    10741 ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa agccagttgt
    10801 ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa ctgttggcga
    10861 gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg tgtgtacata
    10921 gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt ttttgagttc
    10981 tgttttcggc ttttataatt
//
LOCUS       DAZGXZ010000161        10913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000161 DAZGXZ010000000
VERSION     DAZGXZ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.161"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            43..759
                     /gene="nanC"
                     /locus_tag="ACHKJF_004507"
     CDS             43..759
                     /gene="nanC"
                     /locus_tag="ACHKJF_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP0951756.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            779..1885
                     /gene="nanM"
                     /locus_tag="ACHKJF_004508"
     CDS             779..1885
                     /gene="nanM"
                     /locus_tag="ACHKJF_004508"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0951757.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1950..2930
                     /gene="nanS"
                     /locus_tag="ACHKJF_004509"
     CDS             1950..2930
                     /gene="nanS"
                     /locus_tag="ACHKJF_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0951758.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(2938..3042)
                     /locus_tag="ACHKJF_004510"
                     /pseudo
     CDS             complement(2938..3042)
                     /locus_tag="ACHKJF_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3142..4014
                     /locus_tag="ACHKJF_004511"
     CDS             3142..4014
                     /locus_tag="ACHKJF_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP0951759.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRRNYPY
                     EGFVEWHRKEVFRG"
     gene            4184..6259
                     /locus_tag="ACHKJF_004512"
     CDS             4184..6259
                     /locus_tag="ACHKJF_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0951760.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVATFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            6252..7595
                     /locus_tag="ACHKJF_004513"
     CDS             6252..7595
                     /locus_tag="ACHKJF_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFP0951761.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNALNRHKFFVDYDDYMPDCAANRLLHSALDKLLSLKLSSENQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAATYSLVKHGLKDCFKLRPDLLIQSHKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     AQHFAFSETLKLWVVPYRIMAKRGERMMWPNDVSCTMSPKLDRMAASVSYSGGMR"
     gene            7592..7978
                     /locus_tag="ACHKJF_004514"
     CDS             7592..7978
                     /locus_tag="ACHKJF_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951762.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLIGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            8029..10176
                     /locus_tag="ACHKJF_004515"
     CDS             8029..10176
                     /locus_tag="ACHKJF_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0951763.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NQRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFDWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WQDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            10176..>10913
                     /locus_tag="ACHKJF_004516"
     CDS             10176..>10913
                     /locus_tag="ACHKJF_004516"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0951764.1"
                     /translation="MATIFKRTPEQLSERRIDPWHYQPRFERQINKIKDNLGALPLEV
                     VIDSKRGVASGATPLGANYLEKGRVKFYRTSEVEEMFLQAEEAVFINDEDDEKLKRSR
                     LVDGDVLLTITGAKFGKSAVVSAKHLPANISQHSVRFKPDPKKVDAYFLVAYLNSKTG
                     QVAIWKEAYGATRPAIDFPSVRSLAVPKVLPLAQKYIGDKVRQAEQLRAWAKRLEGMA
                     DRKIKDLFHFNLVDSLTLKPRRMKQQVL"
BASE COUNT         3157 a         2425 c         2571 g         2760 t
ORIGIN      
        1 ggctttgtat tgctttctgt atcctacaaa tgagtgaaat ttatgaaaaa ggctaaaata
       61 ctttctggcg tattattact gtgcttttcg tccccattaa tttctcaggc tgcgacactg
      121 gacgtacgtg gtggatatcg tagtggaagc cacgcctatg agactcgact caaagtcagt
      181 gagggatggc aaaatggatg gtgggcaagc atggaaagta atacctggaa taccattcat
      241 gataataaaa aggaaaatgc cgcactcaat gatgttcagg ttgaagttaa ttacgcgatt
      301 aaacttgatg atcaatggac ggtgcgcccg ggaatgttaa cgcattttag cagcaacggc
      361 acacgctacg gaccctacgt aaaactgtcc tgggacgcga caaaagatct taattttggc
      421 attcgctatc gttacgactg gaaagcttac cgacaacaag acttatccgg tgatatgtct
      481 cgtgataacg ttcatcgttg ggatggatat gtcacttacc atattaatag tgatttcacc
      541 ttcgcatggc aaacgacgct atacagcaaa cagaacgatt atcgctatgc aaaccataag
      601 aaatgggcga cggaaaatgc atttgttcta caataccata tgacgcccga tattacgcca
      661 tacatagaat atgactacct ggaccgtcag ggtgtttaca acggcagaga taatttatcg
      721 gaaaacagtt atcgcattgg tgtgtcattt aaactgtagt agacaggaga cagtcacaat
      781 gaataaaaca ataacggcgc ttgctatcat gatggcttca tttgccgcaa acgcgtctgt
      841 attacctgaa actcctgtgc catttaaaag tggtaccgga gcaattgata acgacactgt
      901 ctacattggt ttaggtagcg caggtacggc atggtacaag ctggatacac aggccaaaga
      961 taaaaaatgg acagcgttag ctgcattccc tggtggacca agagatcaag caacctcggc
     1021 atttattgat ggcaatctgt atgtgtttgg cggcattggc aaaaatagca agggcttgac
     1081 tcaggtattt aatgacgtac acaaatacaa ccccaaaacc aatagttggg ttaaattgat
     1141 gtcgcatgca ccgatgggca tggcgggtca tgtgactttt gtacacaacg gcaaggctta
     1201 tgttactggc ggtgttaacc agaatatctt caatggctat tttgaagatc tcaacgaggc
     1261 tggaaaagat tcagcaacta tagacaagat caatgcccat tattttgaca aaaaagcaga
     1321 agattatttc ttcaataagt ttctgttgtc ttttgatccc tcaacacagc aatggagtta
     1381 cgctggcgaa tctccctggt acggaacggc tggtgcggcg gttgtgaata aaggtgataa
     1441 aacctggctt attaatggcg aagccaaacc aggattgcga acggatgccg tatttgaact
     1501 tgatttcacc ggtaataatt taaaatggaa taagcttgct cccgtctcat caccagatgg
     1561 cgtcgctggc ggttttgcgg ggataagcaa tgattctctt atatttgccg gaggggccgg
     1621 attcaaaggt tcacgagaaa attaccagaa cggaaagaac tatgcgcatg aaggcctgaa
     1681 aaaatcatat agcactgata ttcatctttg gcataacggg aaatgggata aatcgggtga
     1741 attatcgcaa ggtcgggcct acggagtatc attgccctgg aataatagtc tattgattat
     1801 tggcggtgaa actgcaggcg gcaaagcggt gacggattca gttttgatct ctgtgaagga
     1861 taataaagtc acagtacaaa attaacgctt cagggccccg gtaaaggggc cctgctatca
     1921 atttgtcatt tcaattacgc gaaatttata tgaacgcaat aatatcgccc gattattact
     1981 atgttcttac cgttgctggt cagtctaatg ccatggcgta tggcgaagga ctgccattac
     2041 cggacaggga agatgcgcct catcccagaa ttaaacaatt agcgagattt gcgcatacgc
     2101 atcccggagg cccgtcatgt cactttaacg acattattcc actgactcac tgcccacacg
     2161 atgttcagga tatgcagggt tatcaccatc ctctggcaac gaatcatcaa acacagtacg
     2221 gcaccgttgg ccaggcactg catattgcac ggaaattact accctttatt cctgataatg
     2281 caggggttct catcgttcca tgttgccgtg gcggatcggc ttttaccgcg ggcagcgaag
     2341 ggacatattc agaacggcac ggagccagcc atgatgcttg tcgttgggga acggatactc
     2401 cgctgtacca ggatttagtc agcagaacgc gagccgcact ggcaaaaaat ccgcagaaca
     2461 aattcctcgg cgtatgctgg atgcaaggcg aatttgactt aatgaccagt gactacgcgt
     2521 cacaccctca acactttaat catatggttg aagcctttcg tagggatcta aaacaatacc
     2581 attctcagct taataatatt actgacgcgc cgtggttttg cggcgatacc acctggtact
     2641 ggaaagaaaa tttcccgcat tcgtatgaag ctatttatgg caattatcaa aataatgttt
     2701 tagccaatat tattttcgtc gacttccagc aacaaggtga aagaggactg acgaacgcgc
     2761 ctgatgaaga tccggacgat ttaagcacgg gatattacgg ttcagcgtac cggtcaccgg
     2821 agaactggac gacggcactg cgaagcagtc atttcagcac ggcagcccgt cggggggtta
     2881 tttctgacag gtttgtagaa gcaattttgc agttttggcg cgaaaagtga gcgttcatta
     2941 atttgattta ttcgcatcca tcaacgcttt taactgctcc ggcgtaaacg gtggtttgcg
     3001 ccggtgcagc atcgcatggc aattagggta cacaggcagc aaaaaaacaa atgagatcaa
     3061 ggtaatatat cttcacttgc aggcttgaac agcagaaaat ttgcgagcat gatcgcaatt
     3121 atttcactgc cagggatatc aatggcttcc agtaaatcgc tacagcaggc aattgccaac
     3181 ataaaaatct ggcacaaggg tgaacagcgc gcgccgcata agccattgtt attgctatac
     3241 gtattagcgg gatacctgaa tggacatcca cgccttttcg attatggatc ggaaatctac
     3301 gaacctctgc acagtttact ggaacgtttt gggccacagc gttcacagta ccgacctgat
     3361 atgccgttct ggcgattaca aggcgacgga ttctggcaat tacacaatgc cgagctttgc
     3421 tcaacagcag ggagcagccg acaaccgcca gtgaaggaac tgaacgaata ccacgttgca
     3481 ggcggtttcg atgaacagca ctacgccctg gtaaccggca ataaaaaact catcaatact
     3541 ctggcgcaac agatactcga agcgcacttc accgagagta ttcaggaaga aatcgctgac
     3601 gagctgggtt ttgatctcca gcaaatccgt aaacagcgcg accctttatt tcgcaaaaat
     3661 gtattacgcg cttataacta ccagtgcgcg atatgtggct ttaatatgcg ccacgacgac
     3721 accaccgtag cgttggaagc ggcgcatata aaatggaaac agcaaggagg tccttgtgaa
     3781 attcctaatg gcctggcgct ttgcgcaatt caccataaag cctttgataa aggatcgatt
     3841 ggcctggatg aaaatatgcg ggtactggtc tcggatgcgg ttaacggcgg agggatcgtt
     3901 gagagattat tctgggattt tgacgggaaa actattgcgt tgccgcaggt gcgtagaaat
     3961 tacccttatg aggggtttgt ggagtggcat cggaaagagg tttttagggg gtgaggttga
     4021 taacagcatt caccctacaa ccttacattg tatttaaatt agagctgagt taacagatag
     4081 ttgtatatat aacaggcact tgtcacaacg attaactcaa tatagttaga atacctcata
     4141 ttaattaaaa aataagtaat gtcacaacag aggaaaagtc acgatgactc ttgtagatag
     4201 tgtcgaagca ggtaggctaa cgattagtga attgattgat gccctggcga aagataaaaa
     4261 ttacacagct tccagatggt atcagcgata ccgtgcattt acgactttgc tacagcaaac
     4321 ctcaactttt gctgagcctg caacagatgg tctggtcaaa cagctttggt atgagcgtga
     4381 caacggcatt gcaagtattc gccagggcgt tccatcctta gcagaatatc agcaaagcct
     4441 gccactgctt agagaactaa ctgaacgaat tcggcaacag ccggatgaag aaacttacca
     4501 atatgttggc aatgcacttc aacaagctaa agaaaacgga cttctcaagc gtatgtattg
     4561 gagtttgaga aatcgcgtct ttgccgcgtt ctcgccagaa aactacacca gtactgtgga
     4621 tgagaatgct tttaataaag cagcagaatt cttaaatcag cacttccatc tcggtttggt
     4681 actgaccgga aattggttac agaaaaacta tgaattgaaa caagccatac acgcccaatc
     4741 tcctgataca gatccttatt atgtgaatat ggccatctgg catctctatg aattgctccg
     4801 tgaacgcgat aatgaacaaa agcaggagaa agtagctagc actacatcca taacccgcag
     4861 tgagcccatc gagaacaaga tcatcctaca ttcaccaact aacgtgatct tctttggccc
     4921 ccctggcact ggcaagacct tcaggttgca gcaaaaaatg aaagagtaca cttctcatgc
     4981 tgttcccgct gatcgtgatg cctggttgga ttctcgcctt gaatcgttga actggatgca
     5041 ggttataacg ctggtgctgc tcgatcttgg gaaacgagcg aaagttcgcc aaattattga
     5101 acatatgtgg tttcaacgta aggcattatt aaacggtcgt aatggcaatc tatcgaatac
     5161 tgcctgggca gctttgcaat cctatacagt tcccgagtcg ttaaccgttg attataagaa
     5221 tcggcgtgag cctgccgtat ttaacaaaac agataacagc gaatggtttc tagttgattc
     5281 acagctcgag caagtggagg atttggtaga gctctacgcc gaacttaaac gtggccctaa
     5341 atctgccgaa gccatccagc gttttgcggt ggctacgttc caccaatctt acggctatga
     5401 agaatttatt gaaggtatac gcgctcgctc tgacgagagt ggcaatatct cttatcccat
     5461 tgagccgggt atctttatgc gcctttgcca acgtgcgaat gccgatccag gacatcgcta
     5521 cgccattttc attgatgaga tcaatcgcgg taacatatcc aagatctttg gtgaactaat
     5581 ctcactcatt gaagtagaca agcgtgcagg catgcccaat gcgatgagcc tgcaactggc
     5641 ttatagcggt gatcacttca gcgtacccgg caatgtcgat atcatcggag ccatgaatac
     5701 agcggaccgt tctttagctc tgatggacac ggctttgcgc cgtcgctttg actttgtcga
     5761 aatgatgcct gatctctctt tactgagtga agctaaggtg aaaggcatag agctcgagtc
     5821 gttgttagag aaactcaata gccgcatcga ggctctttac gatcgtgaac atacgctggg
     5881 gcatgcgttc tttatgccgg taaaaaatgc actcgatgcc ggtgatgaag aagctgcgtt
     5941 taaacaattg aagatcgcat tccagaaaaa gatcattccg cttttacagg aatacttttt
     6001 cgatgactgg aacaagatcc ggttggtgct ggcagacaat caaaagcaag acgacaacct
     6061 gcaattcgtg attgagaaaa ccgacgatct cgatacgctt tttggtaaca accatggttt
     6121 acgacgccat gatcagcaat caacagctta tgagctcaaa gatttcgatc aagagatctg
     6181 gaatattcca caggcttatc gttcaattta tcagccccaa cagactcccc ttgatgagca
     6241 ggcagtaaat catgggtgaa gttatctccg tttttgaata tgacctcttg gggagtggaa
     6301 aagcggcttc agttggcgca aaaccggtgc ctccacaggt ctttaattat ttagaagcgc
     6361 ttagcctggc aagtaatcaa gggagccagt tcctcaaact cacctctcgc tcaggcttca
     6421 aattgcttca ggttcaaaac tacgcaggga tgctttcaac acctcacggt ttccagcttg
     6481 agatactgcc caaggttggt aaaaacctca cagcggctaa tgcacgtcaa acattgctga
     6541 cgatgctaag ccatctgcct gggtttcgac atattcaaac ccagcaagcc actcttcagg
     6601 ctcagcgtat gcctttgctt gagattttta ttaaccagtt tttgcatagc gtcagccaat
     6661 tgcttaaaca gggtttacgc tccgactatg tgagcaagca aggtaatctg gcttttatga
     6721 agggcaagct gatgctttct gcacagctgc gacataacgc gctgaatcgc cataagtttt
     6781 ttgtcgatta tgatgactac atgcctgatt gtgcagccaa tcggctctta cactccgcgc
     6841 tagataagct tcttagtcta aagctgtcat cagagaatca acgctggctt tacgaactgc
     6901 gctttgcgtt tgacggtatt ccacttagcc gggatattga acgtgatata aacaacttac
     6961 gcctcgagcg tggtatggct cattacaacg agccaatggc atgggcgcaa ttgatcctga
     7021 ggggaatgag cccgagtgct ttgcagggaa acaccaaagc gatatcactt ttgttcccta
     7081 tggaagcggt atttgaatcc tttgtggcac agaccctacc cgacgagtta ccacctcacc
     7141 tcaaagtttt acctcaagca gcaacttatt ctttggtaaa gcatggactt aaagattgct
     7201 ttaagcttcg ccccgacttg ctgattcaat cacacaaacc agttcaaacc gaaatggtga
     7261 tggatacaaa atggaagctg gtgaatagca gccagcagac aaaatcactc tatggactgg
     7321 cacaagctga cttctatcaa atgtttgcct acggacaaaa atatcttggt gggaatggcg
     7381 aaatgtatct gatttaccct gcgcatgatg actttagcca acctatagcg cagcattttg
     7441 ctttctcgga gactttaaaa ttatgggttg tgccataccg gataatggca aaacgcggtg
     7501 agaggatgat gtggccgaat gacgtgtcct gtacaatgag ccccaaactg gatcggatgg
     7561 ctgcttcagt atcctatagt ggaggcatga gatgacccat gaataccagc gacaaagcct
     7621 ggaacaacta ataactcagc aaacagcaac tcccagaagc gccgccgttg tatttaatga
     7681 gaaccaaacc ctctggcaaa cacttggctt tagccagtct caagtttcgc tatggcttgc
     7741 ctcattgcct caaagtaatc atctaatagg gcccacaacg gcaacgtacc aggttatacc
     7801 tgatatcgcg gcacatttgg tatcattgtt gcatcaagct ggtgggcgca tgccccttgc
     7861 acaagtactg aaaaagctcc ctgctggtat tacgaccagt gaacagcaga tacgcaaact
     7921 ggcacagaag cacgcgcaga tagagattaa aggccccttg ctggtactga taaattagcc
     7981 ggcatttgta gtaaccattg gctgtattaa aggattttag aaaacatcat ggcaaaagaa
     8041 gcaaaaaaac aagacccgca acagcagcta gaagcactgt tcaataaact ggtcaaacag
     8101 cactccgagt ttactcagcc attggtcgaa ttacccgagc atgaattcgc caaggctggt
     8161 ctcattcaag gcctggttga aggcaactta acgccaccag tcatgttgat tgcgctgaca
     8221 gaaggccatt gggaatacgc cgctgacact gcaaagtttg atgatttagc ctatcagctg
     8281 ttaagtgata tcgaaggtga ctggccagta tatgccgttg tggacgatgg cttgaaccaa
     8341 cgtattttgg ccttgttcgg tgccgatgga tctgacggta cccacggtgc agataccctg
     8401 cctggtctgg aagaattacg tagctttgat cgcatggagc gtgatccaac ctttcgctgg
     8461 tcgatgcgtg tttatacacg cttaatgcag cgatttgatg ccttccatga aaatgtttat
     8521 cgcgttacca aagacaaggt aaacgacaag aacgacatta ttgaagaagt agctaagctt
     8581 ctgtttttag aaactttccg cctgcatcac gatgaagacc ttacctttaa agatgatgag
     8641 ggaaataccc tgcgttttcg tgatgtcttt gattggcaat atgtggagag ccacggcgat
     8701 aaagccgttg cccaaatcaa agccgccttt gaggaattta aaagccacga aaactacgtt
     8761 gtgatctcag atgatggctc acgtaacccc atctttagta aagaaacaca cctgcgactg
     8821 tctgttgcga aaaactatca ggatttgctg gaagcaatac aaaacctggg tccggtaaaa
     8881 accaacgacg gtaaaatcgc gaaagaacac ggcacgttgg ctgatgtgtc aggagattta
     8941 ctaggcagag tgtttgatgt attcctacgc gctaactttg aatccaaagg tggccttggc
     9001 gtttatttaa caccaaaccc agtaaaacag gccatgctag aaattgctat gcacgacatc
     9061 gacgatgacg atgaaatgcg atcgcgctta gccaatggcg attttcgttt ctgcgacccc
     9121 acctgtggct cctttggttt tggctcggtg gcgttaagcc agattgataa atggatcgat
     9181 tttaagctgg tgctggccga cgataaaaaa gaatcattga aacaaaaatt acgcgactgt
     9241 gcatttaccg gtgccgatgc tgcaccacgc atggtaatgc tggcccgcgt gaatatggca
     9301 ttgcaaggtg cccccaaagc gcagattttt tataccgaca actcactcac tactaacgct
     9361 ctgaagccga atagctttga cctgatttgc accaacccgc cgttcggtac gcctaaattt
     9421 gataaaggca aaaatggtca aaactcaaaa gccaattacg aagccaatat ggagcaagtg
     9481 ctcggtggtt ttcgtccgac caaaaaagtc gttgatagct acaacgcctg gtatgaccac
     9541 gtgaaaatga agtggcagga tatcggtgat ttagagctgg atgaaaacgg cgagcctaaa
     9601 tgggcgggct accgtaccga tttgcgcaga acgggcggga ctgataaaaa gccgttttac
     9661 agcctgcagc ctacaaccgc agggctcgcg ctgggcagca agcccgatag caagggcaac
     9721 tggcaaccgg taggtgccac tattgaccca gcagtacttt ttatcgaccg ctgcctgcaa
     9781 ctgcttaaac ccggtggccg cctgttaata gtgctgcctg atggtatttt atgtaactcc
     9841 ggcgaccgtt atgtccgtga gtacattatg gggaaaaaag acgaaaaaac cggcgagttt
     9901 gttggcggta aagctattgt aaaagcagta atttcattgc cgagcgactg ctttaaactc
     9961 tccggtacgg gggccaaaac ctcgattttg tatttacaaa aacgccatgc taaccccaac
    10021 cagccagagc aatttttgcc cgagccacaa accgacgtgt ttatggcagt ggctgaaaca
    10081 ttgggttatg tggtgaaaaa caatatcgaa gactataacg ccggtgtcgc aaacgatctg
    10141 gataagattg tatcggctta taagcggggt gagtgatggc aactattttt aaaagaacac
    10201 ccgaacaatt gtctgaaagg cggattgatc cttggcacta tcaaccgcga tttgaaagac
    10261 aaattaataa gataaaagac aacctaggtg ctttgcccct agaagtggta attgattcaa
    10321 agcgcggggt ggctagtggc gcaacacctt taggagcaaa ttatttagaa aaaggccgtg
    10381 ttaagtttta tcgcacttct gaagttgaag aaatgttctt gcaagctgaa gaagctgttt
    10441 ttattaacga tgaagatgat gaaaaattaa agcgcagccg attagtggat ggcgacgttt
    10501 tacttacgat tactggtgca aaatttggta aatcggctgt tgtgtcagct aaacatctac
    10561 cggccaacat tagtcagcac tcagtcagat tcaagcccga tcccaaaaaa gttgacgcct
    10621 atttcttggt ggcatatttg aatagcaaaa ccggacaagt agcgatctgg aaggaagcat
    10681 atggcgcaac tcgccctgca attgacttcc caagtgtccg ctcgctagcg gttcctaaag
    10741 ttttacctct agcccaaaaa tacatcgggg ataaagttcg ccaagccgag cagttacgcg
    10801 cttgggcgaa acgacttgaa ggaatggctg atagaaaaat taaagattta tttcacttca
    10861 accttgttga ttcattaaca ttgaaaccta gaagaatgaa acaacaggta tta
//
LOCUS       DAZGXZ010000162        10832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000162 DAZGXZ010000000
VERSION     DAZGXZ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.162"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(67..1731)
                     /gene="glnS"
                     /locus_tag="ACHKJF_004517"
     CDS             complement(67..1731)
                     /gene="glnS"
                     /locus_tag="ACHKJF_004517"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP0951765.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1934..3880)
                     /gene="nagE"
                     /locus_tag="ACHKJF_004518"
     CDS             complement(1934..3880)
                     /gene="nagE"
                     /locus_tag="ACHKJF_004518"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP0951766.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4213..5013
                     /gene="nagB"
                     /locus_tag="ACHKJF_004519"
     CDS             4213..5013
                     /gene="nagB"
                     /locus_tag="ACHKJF_004519"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0951767.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5073..6221
                     /gene="nagA"
                     /locus_tag="ACHKJF_004520"
     CDS             5073..6221
                     /gene="nagA"
                     /locus_tag="ACHKJF_004520"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0951768.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6230..7450
                     /gene="nagC"
                     /locus_tag="ACHKJF_004521"
                     /gene_synonym="nagR"
     CDS             6230..7450
                     /gene="nagC"
                     /locus_tag="ACHKJF_004521"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP0951769.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7498..8250
                     /gene="nagD"
                     /locus_tag="ACHKJF_004522"
     CDS             7498..8250
                     /gene="nagD"
                     /locus_tag="ACHKJF_004522"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP0951770.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8507..10171
                     /gene="asnB"
                     /locus_tag="ACHKJF_004523"
     CDS             8507..10171
                     /gene="asnB"
                     /locus_tag="ACHKJF_004523"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP0951771.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10552..10628
                     /locus_tag="ACHKJF_004524"
     tRNA            10552..10628
                     /locus_tag="ACHKJF_004524"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10586..10588,aa:Met,seq:cat)
     gene            10637..10721
                     /locus_tag="ACHKJF_004525"
     tRNA            10637..10721
                     /locus_tag="ACHKJF_004525"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10671..10673,aa:Leu,seq:tag)
     gene            10745..10819
                     /locus_tag="ACHKJF_004526"
     tRNA            10745..10819
                     /locus_tag="ACHKJF_004526"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10777..10779,aa:Gln,seq:ttg)
BASE COUNT         2682 a         2813 c         2757 g         2580 t
ORIGIN      
        1 gggattgttt gctatttctt gccggatgcg acgtaaatgc ctcacccggc ctggtagaac
       61 ttaaaattac tcgcctactt tcgcccaggt atcacgcagc ccaacggtgc ggttaaatac
      121 cggtttttcc gctgtggaat ggcggctgtc gaggcagaag taaccttcac gctcaaactg
      181 gaatgcttta cccgcaaccg catctttcag cgacggttca gcaaagccct gtttgatcac
      241 cagcgattcc gggttaatca ccgacaggaa atcatccgca gcaccagggt tcggcacgct
      301 gaacagacgg tcgtacagac ggatttcaac cggcagcgca tgtgccgcgc ttacccagtg
      361 aataacgcct ttcactttac gaccatctgc cggatccttg cttaaggtgt cggcgtcata
      421 agtacagaag atggtggtga tattgccttc ggcatctttc tccacgcgtt cagccttaat
      481 gacgtaagca ttacgcagac gcacttcttt acccagcacc agacgtttgt actgcttgtt
      541 agcttcttcg cggaaatcgg cgcgatcgat ccaaatctca ccgctaaacg gcacctgacg
      601 gctccccatt tccggtttgt tcggatggtt cggcatggtg accatttcgc cttcgccctg
      661 atagttttcg ataaccagtt tcaccggatc gataaccgcc attgcgcgcg gcgcattttc
      721 gttgagatct tcacggatgc aggattccag cgacgccatc tcaatggtgt tgtcctgctt
      781 ggtcacgcca atgcgtttgc agaactcacg aatagaagcc gcagtgtaac cacgacgacg
      841 cagaccagaa atggtcggca tacgcgggtc atcccagcct tcaacgtgct tgtcggtcac
      901 cagcaggttc agcttacgct tggacatcac agtgtattcc agattcaggc gcgagaattc
      961 gtactggcgc gggtgaacag gaatggtgat gttgtccagc acccagtcgt acaggcgacg
     1021 gttgtcctgg aactcaagcg tacacagaga gtgcgtaata ccttccaggg catcgctgat
     1081 gcagtgggtg aagtcgtaca tcgggtagat gcaccacttg ttgccagtct ggtggtgttc
     1141 agcaaattta atacggtaca gcaccggatc gcgcatcacg ataaacggtg aagccatgtc
     1201 gattttcgca cgcaggcagg ctttaccttc ttcaaaacca ccggcacgca ttttttcgaa
     1261 cagcgccagg ttctcttcaa cgctgcggtc gcggtacggg ctgtttttac ccggttgcgt
     1321 cagggtgccg cggtattcgc ggatctgttc cggcgtcagt tcatcaacgt acgccaggcc
     1381 tttattgatc agttcgatcg cataggcgtg gagctgatca aaataatcgg aggagtaacg
     1441 gacgttacca gaccagtgaa aacctaacca ctctacgtcg tttttgatcg actcaacata
     1501 ctcgatatct tcttttaccg ggttagtgtc gtcgaaacgc aggttgcact ggcctttata
     1561 gtcctgggcg atcccgaagt tcaggcagat agatttcgca tggccaatat gcagatagcc
     1621 attcggctcc ggcgggaaac gggtgtgtac tgtggtgtgc ttaccactgg ccagatcttc
     1681 atcgatgatc tgacggataa agttagtcgg gcgggcttct gcctcactca tcgtggattc
     1741 ctcaaagcgt aaacaacgta taacggcgta tgatcttata agcgggacag gctgacaact
     1801 gttagttttt taaaattgat aatctaacaa ggattatggg gatgattgat gaaaaaaaaa
     1861 gcggcagaga agatctccac cgcttgtcat tgttggatgc gacgctcaag cgtcgcatca
     1921 ggcataaagc agattacttt ttgatttcat acagcggtgt ttgacccgcc acaacatggc
     1981 cctgagcttt aatgatcaag ccactgaaat cgtcgatatt gctgcaaacc accgggctaa
     2041 tcatcgagcg ggcgttagcg ttcaggtaat ccagatccat ttccagaatc ggttgccctg
     2101 cgcttacctg cgcaccctct tccaccagac gtttaaagcc tttaccttcc agcgctacag
     2161 tgtcgatacc catatggacg acgatctccg cgcctttttc ggtttccagg cagaacgcgt
     2221 ggttggtgtt gaagattttc acgattgtcc ctgcggctgg tgatacgacg attttatctg
     2281 tcggtttcac cgccacaccg tcacccaccg ctttgctggc gaatgcttcg tcaggaacct
     2341 gatccagtgc cacgacatca ccggtaatcg gcgataccag ctccgcgata gataccgcgt
     2401 ttggtacagc ctgcggtttt gctacaggcg cggcagttgc cggagttgct tcagctgacg
     2461 cagcggctac cggaccacgg gcaacgactt tcttcatcgc atcgccgatg gattctgctt
     2521 tcgcgccaac aatcacctga atagtctgtt tgttcagttt cactacacca gaagcaccca
     2581 gacgtttaca catcgtatcg ttgacacggg cagagtcagc cacggtcagg cgcagacggg
     2641 tgatacaggc gtcaattgct ttcaggttgt cagtgccgcc aaccgcagca atatagttgg
     2701 tcgccagttg attgaggcct tcttcagtgt tgctgttggc ttcttcagta acgatctcgt
     2761 cttctttatc ttcacgaccc ggcgttttca ggttgaacat gcggataacc aaactgaaca
     2821 ccacgaagta gatagcgaag aagacaacac ccataaccag cagcatccag acgttctggc
     2881 tggcggccgg caggttatac atcaacgcgt agtcgatagc ccccgcagag aaagagaagc
     2941 cagcatggat acccagcagt gttgccacaa acaggctgat accggtcagc agtgcgtgca
     3001 ggaggtacag cagcggtgca aggaacatga acaggaattc cagcggctca gtcacaccgg
     3061 tcaggaacgc agtaacagca acagaaagca gcatcccgcc aaccatcgga cgacgctctt
     3121 tcggtgctgc gaagtacatc gccagcgccg cacccggcag accgaacatc ataatcggga
     3181 agaagccgga catgaacatc cccgcggtgc cgtcaccggc atagaagcgg ttgatatcac
     3241 cgtggaaaac cgtacccgcc gcgttggtga attcaccaat ctggaaccag gcgatggtgt
     3301 tcagcacctg atgcagaccg gttgggatca gcagacggtt gatgaaacca aagataccgg
     3361 aacccagcgc gcccgcagaa acgatccact cgcctcctgc atggatagcg tgctgtaccg
     3421 gcggccagac gtaaccaaaa atggccgcca gcaccaggca gaagaagccg gtggcaatcg
     3481 gcacaaagcg tttgccgccg aagaaactca ggaagtccgg cagtttaata tcggaccaac
     3541 ggttataggc tgcgccacca accagaccgg taatgatacc cgccagtaca cccatgttaa
     3601 tttctgggtt gatggtcacc atcgctttgg ttaacacaaa gtaacctacc gcacccgcca
     3661 gcgccgccgc acctgcgctg tctttcgacc agctggatgc cacaccgatg gcgaagatta
     3721 atgcgaggtt atcaaaaatc gcaccgcccg cctgggcaat aaacgcaacg ttaagtaaat
     3781 ctggctgacc gaatcgcagc aacagtgccg ccaccggcag caccgcgata gggagctgta
     3841 acgccctacc gagtcgctgg aaaaaaccta aaatattcat cttattcccc ctacgagaac
     3901 cctatttggc tcgtttaaag ccgtattttt attttgctgc aaattgtact gccgatgttc
     3961 tgtaatcaga ttgttagatc atctgctaca gagtgtgtga aaatttaatt cgtatcgcaa
     4021 attaaacgcg tgtcttttgt gagttttgtc accaaatatc gttatcatca ctccctttta
     4081 ctggctaaag cagaaaactt attttatcat tcaaaaaatc aggtcggatt gacgcctgtc
     4141 tgcgcaaatc caggttacgc ttaaagatgc ctaatccgcc aacggcttac attttactta
     4201 ttgaggtgaa taatgagact gatccccctg actaccgctg aacaggtcgg caaatgggct
     4261 gctcgccata tcgtcaatcg tatcaatgcg ttcaaaccga ctgccgatcg tccgtttgtt
     4321 ctgggcctgc cgactggcgg cacgccgatg accacctata aagcgttagt cgaaatgcat
     4381 aaagcaggcc aggtcagctt taagcacgtt gtcaccttca acatggacga atatgtcggt
     4441 ctgccgaaag agcatccgga aagctactac agctttatgc accgtaattt cttcgatcac
     4501 gttgatattc cagcagaaaa catcaacctt ctcaacggca acgccccgga tatcgacgcc
     4561 gaatgccgcc agtatgaaga aaaaatccgt tcttacggaa aaattcatct gtttatgggc
     4621 ggtgtaggta acgacggtca tattgcattt aacgaaccgg cgtcttctct ggcttctcgt
     4681 actcgtatca aaaccttgac tcatgacact cgcgtcgcga actctcgttt ctttgataac
     4741 gatgtcaatc aggtgccaaa atatgccctg actgtcggtg ttggtacatt gctggatgcc
     4801 gaagaagtga tgattctggt gctgggtagc cagaaagcac tggcactgca ggctgcagtt
     4861 gaaggttgcg tgaaccacat gtggaccatc agctgtctgc aactgcatcc aaaagcgatc
     4921 atggtgtgcg atgaaccttc caccatggag ctgaaagtta agactttaag atatttcaat
     4981 gaattagaag cagaaaatat caaaggtctg taattgttat ccctgccctg gctccttgct
     5041 cagggcaata ttttttaaaa tcgggggtca gaatgtatgc attaacccag ggccggatct
     5101 ttaccggcca cgaatttctt gatgaccacg cggttgttat cgctgatggc ctgattaaaa
     5161 gcgtctgtcc ggtagcggaa ctgccgccag agatcgaaca acgttcactg aacggggcca
     5221 ttctctcccc cggttttatc gatgtgcagt taaacggctg cggcggcgta cagtttaacg
     5281 acaccgctga agcggtcagc gtggaaacgc tggaaatcat gcagaaagcc aatgagaaat
     5341 caggctgtac taactatctg ccgacgctta tcaccaccag cgatgagctg atgaaacagg
     5401 gcgtgcgcgt tatgcgcgag tacctggcaa aacatccgaa tcaggcgtta ggtctgcatc
     5461 tggaaggtcc gtggctgaat ctggtaaaaa aaggcaccca taatccgaat tttgtgcgta
     5521 agcctgatgc cgcgctggtc gatttcctgt gtgaaaacgc cgacgtcatt accaaagtga
     5581 ccctggcacc ggaaatggtt cctgcggaag tcatcagcaa actggcaaat gccgggattg
     5641 tggtttctgc cggtcactcc aacgcgacgt tgaaagaagc aaaagccggt ttccgcgcgg
     5701 ggattacctt tgccacccat ctgtacaacg cgatgccgta tattaccggt cgtgaaccgg
     5761 gcctggcggg cgcgatcctc gacgaagctg acatttattg cggtattatt gctgatggcc
     5821 tgcatgttga ttacgccaac attcgtaacg ctaaacgtct gaaaggcgac aaactgtgtc
     5881 tggttaccga cgccaccgcg ccagcaggtg ccaacattga acagttcatt tttgcgggta
     5941 aaacaatata ctaccgtaac ggactttgtg tggatgagaa cggtacgtta agcggttcat
     6001 ccttaaccat gattgaaggc gtgcgtaatc tggtcgaaca ttgcggtatc gcactggatg
     6061 aagtgctgcg tatggcgacg ctctatccgg cgcgtgcgat tggcgttgag aaacgtctcg
     6121 gcacactcgc cgcaggtaaa gtagccaacc tgaccgcatt cacacctgat tttaaaatca
     6181 ccaagaccat cgttaacggt aacgaggtcg taactcaata agagaaagta tgacaccagg
     6241 cggacaagct cagataggta atgttgatct cgtaaaacag cttaacagcg cggcggttta
     6301 tcgcctgatt gaccagtacg ggccaatctc gcggattcag attgccgagc aaagccagct
     6361 tgcccccgcc agcgtaacca aaattacgcg tcagcttatc gaacgcgggc tgatcaaaga
     6421 agttgatcag caggcctcca ccgggggccg ccgcgctatc tccatcgtca ccgaaacccg
     6481 caatttccac gcaatcggcg tacggcttgg tcgtcacgac gccaccatca ccctgtttga
     6541 tctcagcagc aaagtgctgg cagaagaaca ttacccgctg ccggaacgta cccagcaaac
     6601 gctggaacat gccctgttga atgccattgc tcagtttatt gatagctacc agcgcaaact
     6661 gcgcgagctg atcgcgattt cggtgatcct gccagggctt gttgacccgg acagcggcaa
     6721 aattcattac atgccgcata ttcaggtaga aaactggggg ctggtagaag cactggaaga
     6781 acgttttaaa gtgacctgtt tcgttggtca cgatatccgt agtctggcgc tggcggagca
     6841 ctacttcggt gcaagtcagg attgcgaaga ctccattctg gtgcgtgtcc atcgcggaac
     6901 cggggccggg attatctcta acgggcgcat ttttattggc cgcaacggca acgtcggtga
     6961 aattggccat attcaggtcg aaccgctggg tgaacgctgc cactgcggca actttggctg
     7021 cctggaaact atcgctgcca acgccgctat tgaacaacgg gtgttgaatc tgttaaagca
     7081 gggctaccag agccgcgtgc cgctggacga ctgcaccatc aaaactatct gcaaagccgc
     7141 gaacaaaggc gatagcctgg cctcggaagt gattgagtat gtcggtcgtc atctgggcaa
     7201 aaccatcgcc attgctatca acttatttaa tccgcaaaaa attgttattg ccggtgaaat
     7261 caccgaagcc gataaagtgc tgctccctgc tattgaaagc tgcattaata cccaggcgct
     7321 gaaggcgttt cgcactaatc tgccggtggt acgttctgag ctggatcacc gctcggcaat
     7381 cggcgctttt gcgctggtaa aacgcgccat gctcaacggt attttgctcc agcatttgct
     7441 ggaaaattaa tgtgctttta tagtggcgct tattgttgtc aatattctgg gtagtccatg
     7501 accattaaaa atgtaatttg cgatatcgac ggcgtgctga tgcacgataa cgtcgccgta
     7561 ccgggtgcag cggaattttt gcacgggatt atggataaag gcctgccgct ggtgttgctg
     7621 accaactatc cttcgcagac cgggcaagat ctggcgaacc gctttgccac tgccggtgtc
     7681 gatgtgccgg atagcgtgtt ttatacctct gcgatggcga ctgccgattt cctgcgtcgc
     7741 caggaaggca agaaagcgta tgtggtgggc gaaggcgcac tgattcatga actgtacaaa
     7801 gccggtttca ctattaccga tgtgaaccct gattttgtga ttgttggcga aacgcgttcc
     7861 tacaactggg acatgatgca taaagcggcc tatttcgtcg ctaacggtgc acgctttatc
     7921 gccaccaacc cggacaccca cgggcgcggt ttttatcccg cttgtggcgc gttgtgtgca
     7981 gggattgaga aaatctccgg gcgcaaaccg ttctatgttg gtaagcccag cccgtggatc
     8041 atccgcgcgg cattaaacaa aatgcaggca cattcggaag aaacggtgat tgtcggcgat
     8101 aacttgcgca ccgacattct ggcgggcttc caggcgggtc tggagacgat tctggtgctt
     8161 tctggtgttt catcgctcga cgatatcgac agtatgccgt tccgcccaag ctggatttac
     8221 ccgtcggtcg ctgaaatcga cgttatctga atagcaacca cgtctcaggg cgtggtttct
     8281 tatttttacc catcattaaa atcatcccat tcaataaaac tcgccaaaaa ttatcgatcc
     8341 atcaataaat acatcaccac accctcccct ttttcacaaa tcagcaatca ccattacgtt
     8401 ttttattttt gcactgccaa atcgcttgcg cacggtgctg ctttgcggca tttttttaaa
     8461 caagcaaaca caacaagcaa caaataccag gttaacggag aaggttatgt gttcaatttt
     8521 tggcgtattc gatatcaaaa cagacgcagt tgagctgcgt aagaaagcac tcgagctgtc
     8581 acgcctgatg cgtcatcgtg gcccggactg gtccggtatt tatgccagcg ataacgccat
     8641 tctcgcccac gaacgtctgt caattgttga cgttaacgcg ggggcgcaac ctctctacaa
     8701 ccaacaaaaa actcatgtgc tggcggtaaa cggtgaaatc tacaaccacc aggcactgcg
     8761 cgccgaatat ggcgatcgtt atcagttcca gaccggatct gactgcgaag tgatcctcgc
     8821 gctgtatcag gaaaaagggc cggaatttct cgacgacttg cagggcatgt ttgcctttgc
     8881 cctgtacgac agcgaaaaag atgcatatct gattggtcgc gaccatcttg ggatcatccc
     8941 actgtatatg ggctatgacg agcacggtca gctgtatgtg gcctcagaaa tgaaagccct
     9001 ggtgccagtt tgccgcacga ttaaagagtt cccggcgggg agctatttgt ggagtcagga
     9061 cggcgaaatc cgttcttatt atcatcgcga ctggttcgac tttgatgcag tgaaagataa
     9121 cgtgaccgac aaaaacgagc tgcgtcaggc actggaagat tccgttaaaa gccatctgat
     9181 gtctgacgtt ccttacggtg tgctgctctc gggtggtctg gattcctcaa ttatttccgc
     9241 gatcaccaag aaatacgccg cccgtcgcgt ggaagatcag gaacgctctg aagcctggtg
     9301 gccgcaatta cactcctttg ctgtaggtct gccgggttca ccggatctga aagcagccca
     9361 ggaagtggca aaccatctgg gcacggtgca tcacgaaatt cacttcactg tacaggaagg
     9421 tctggatgcc atccgcgacg tgatttacca catcgaaacc tatgatgtga caactattcg
     9481 cgcttcaaca ccgatgtatt taatgtcgcg taagatcaag gcaatgggca ttaaaatggt
     9541 gctgtccggt gaaggttctg atgaagtgtt tggcggttat ctttacttcc acaaagcacc
     9601 gaatgccaaa gaactgcatg aagagacggt gcgtaaactg ctggccctgc atatgtatga
     9661 ctgcgcgcgc gccaacaaag cgatgtcagc ctggggcgtg gaagcacgcg ttccgttcct
     9721 cgacaaaaaa ttccttgatg tggcgatgcg cattaacccg caggataaaa tgtgcggtaa
     9781 cggcaaaatg gaaaaacaca tcctgcgtga atgttttgag tcatacctgc ccgcaagcgt
     9841 ggcctggcgg cagaaagagc agttctccga tggcgtcggt tacagttgga tcgacaccct
     9901 gaaagaagtg gcggcgcagc aggtttctga tcagcaactg gaaactgccc gcttccgctt
     9961 cccgtacaac acgccaacct caaaagaagc gtatctgtac cgggagatct ttgaagaact
    10021 gttcccgctt ccgagcgccg ctgagtgcgt gcctggcggt ccttccgtcg cgtgttcttc
    10081 cgctaaagcg attgagtggg atgaggcgtt caagaaaatg gacgatccgt ctggtcgtgc
    10141 ggttggcgtt caccagtcgg cgtataagta agaaatgcga aacagccccg gtgaaatccg
    10201 gggctgtttc agttattgag gtgcttgtta attctgatat gcagtaagga aatgttccgg
    10261 gtttgccaca tcactgtttg acaactcatc actgtaggcc agtatttcat agattaattt
    10321 cccaccacta ttgttgaatt ccgaatttca tcctgtacga caaaagaatg gctgtagggt
    10381 ggggaaaaaa cacaaagtaa acaataattg acgaatatag cgccacgctg ttcgcaacct
    10441 aaccaaacag tcactttcga gcaattttcc ttgaaaaaga ggttgacgct gcaaggctct
    10501 atacgcataa tgcgccccgc aacgccgata aggtatcgcg aaaaaaaaga tggctacgta
    10561 gctcagttgg ttagagcaca tcactcataa tgatggggtc acaggttcga atcccgtcgt
    10621 agccaccatc ttttttgcgg gagtggcgaa attggtagac gcaccagatt taggttctgg
    10681 cgccgcaagg tgtgcgagtt caagtctcgc ctcccgcacc attcaccaga aagcgttgta
    10741 cggatggggt atcgccaagc ggtaaggcac cggtttttga taccggcatt ccctggttcg
    10801 aatccaggta ccccagccat cgaagaaaca at
//
LOCUS       DAZGXZ010000163        10630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000163 DAZGXZ010000000
VERSION     DAZGXZ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.163"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            199..1566
                     /gene="dcuC"
                     /locus_tag="ACHKJF_004527"
     CDS             199..1566
                     /gene="dcuC"
                     /locus_tag="ACHKJF_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0951772.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1609..2106)
                     /gene="sspB"
                     /locus_tag="ACHKJF_004528"
     CDS             complement(1609..2106)
                     /gene="sspB"
                     /locus_tag="ACHKJF_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP0951773.1"
                     /translation="MDLSQRTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2112..2750)
                     /gene="sspA"
                     /locus_tag="ACHKJF_004529"
     CDS             complement(2112..2750)
                     /gene="sspA"
                     /locus_tag="ACHKJF_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP0951774.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3145..3537)
                     /gene="rpsI"
                     /locus_tag="ACHKJF_004530"
     CDS             complement(3145..3537)
                     /gene="rpsI"
                     /locus_tag="ACHKJF_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP0951775.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3553..3981)
                     /gene="rplM"
                     /locus_tag="ACHKJF_004531"
     CDS             complement(3553..3981)
                     /gene="rplM"
                     /locus_tag="ACHKJF_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP0951776.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4200..5327)
                     /gene="zapE"
                     /locus_tag="ACHKJF_004532"
     CDS             complement(4200..5327)
                     /gene="zapE"
                     /locus_tag="ACHKJF_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP0951777.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5521..5919
                     /gene="zapG"
                     /locus_tag="ACHKJF_004533"
     CDS             5521..5919
                     /gene="zapG"
                     /locus_tag="ACHKJF_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP0951778.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6073..7440
                     /gene="degQ"
                     /locus_tag="ACHKJF_004534"
     CDS             6073..7440
                     /gene="degQ"
                     /locus_tag="ACHKJF_004534"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP0951779.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7530..8597
                     /gene="degS"
                     /locus_tag="ACHKJF_004535"
     CDS             7530..8597
                     /gene="degS"
                     /locus_tag="ACHKJF_004535"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP0951780.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8660..9598)
                     /gene="mdh"
                     /locus_tag="ACHKJF_004536"
     CDS             complement(8660..9598)
                     /gene="mdh"
                     /locus_tag="ACHKJF_004536"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0951781.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            10033..10503
                     /gene="argR"
                     /locus_tag="ACHKJF_004537"
     CDS             10033..10503
                     /gene="argR"
                     /locus_tag="ACHKJF_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP0951782.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2620 a         2691 c         2640 g         2679 t
ORIGIN      
        1 attcacgagc attatgaata ttatgaatgt gttcttacaa aataatcata agcgcatatt
       61 ttttaatgaa aaatcacctc acctacaatt aaaaacacga catccgcacc ataaatagac
      121 ttgcaaaaaa tataacatca ttgttttcaa tctgccgttt atgggattga ccgttttctt
      181 ttgacacgga gttcaacaat gttcggcata attatatctg tcatcgtatt aattacgatg
      241 ggctatttga tcctgaaaaa ctacaaacct caggtggtgc tggctgccgc aggtatcttc
      301 ctgatgatgt gcggtgtctg gttagggttc ggtggtgtac tcgatcccgc caaaagcagc
      361 ggctacttga tcgtcgatat ttataatgaa atcctgcgca tgctgtccaa ccgcattgcc
      421 ggattggggc tatcgattat ggcggtgggc ggttatgccc gctacatgga gcgcataggg
      481 gccagtcgcg cgatggtgag cttgttaagc cgcccgttaa aactcattcg ctcgccgtat
      541 attattctgt cggcaactta cgtcatcggt caaatcatgg cgcagtttat taccagcgcc
      601 tccggtctgg gtatgttgct gatggtcacc ttatttccga cactggtgag tctgggagta
      661 agtcgtctct ctgcggtggc ggttatcgca accacaatgt ccattgagtg gggaattctg
      721 gaaacgaact ccatttttgc agcccaggtc gcgggaatga aaattgccac ttacttcttc
      781 cactaccagc ttccggtcgc ctcttgcgtc attatctcgg tggcgatctc ccactttttc
      841 gtgcaacgcg cttttgacaa aaaagataaa aatatcaatc acgaacaagc agagcaaaaa
      901 gctctcgata atgtcccgcc gctctattac gccattttac ctgtgatgcc gttaatcctg
      961 atgctcggct cgctgttcct cgcccacgtc gggctgatgc agtcagaact gcatctggtg
     1021 gtggtgatgt tactgagttt gacggtgacg atgtttgttg agttcttccg caagcataac
     1081 ttgcgcgaaa caatggacga tgtgcaggcg ttttttgacg gcatgggtac gcagtttgcc
     1141 aacgtggtaa cgctggtggt cgcgggtgaa atatttgcga aaggcttaac gacgattggc
     1201 actgttgatg cggttatcag gggtgcggag cattctggtc tgggcggtat tggcgtgatg
     1261 attattatgg cgctagtcat tgccatttgt gccattgtga tgggctctgg caatgcgccg
     1321 tttatgtcat ttgccagtct tattccgaat atcgcagccg gactacatgt accagcggtt
     1381 gtaatgatta tgccgatgca ttttgccacg acgctagcgc gcgcggtttc gccgattact
     1441 gcggtggtgg tcgttacgtc aggaattgca ggcgtttcgc cttttgcggt ggtgaagcga
     1501 acagcgatcc ccatggcagt cggtttcgtg gtgaatatga ttgccacaat cacgctattt
     1561 tattaagcca ttaaaagaca aaacaggcca ccagggcctg tttttgtatt acttcacaac
     1621 gcgtaatgcc ggtcgaccac cgcgtggtgg ctgcggaggt tcatcgtcag gatgagtgtc
     1681 atcatcgtga tctggcttgt cgccatcaat aaccgacata acggtttcgt tgtctgccga
     1741 tgcctcttca tcattcatga tgctggtatc ttcatcgtag gcagcttcag gctcaaacat
     1801 tgtgcctgcg ccattttcac gggcgtagat agccagcacg gcagccagcg gcacagaaac
     1861 ctgacgcgga atgccgccaa agcgcgcgtt aaagcgcacc tcatcattcg ccagttccag
     1921 attgccgaca gcacgcggcg caatgttgag tacgatttgt ccgtcacgcg catattccat
     1981 aggaacctgc acgccaggga gcgtcacatc caccaccagg tgcggcgtga gctggttatc
     2041 cagcaaccac tcatagaatg cacgcagcag atagggacga cgtggtgttc gctgtgacaa
     2101 atccatacag attaactccg gcccagacgc atttcacgtt ctgcttcagt taaagaagca
     2161 aggaaagagt cacgctcaaa gacgcgggtc atatagcctt tcagctcttt cgcacccggg
     2221 ccgctgaact cgatgcccag ttgcggcaga cgccacagca gcggagcaag atagcaatcg
     2281 actaggctga actcatcgct caggaagtac ggcttctgac cgaagaccgg cgcaatcgcc
     2341 agcagttctt cgcgcagttg cttacgtgcg gcatctgctt cagaagctga accgttgatg
     2401 atggtgttca tcagcgtgta ccagtctttt tcgatacgat gcatgtacag acggctttca
     2461 ccgcgagcta ccgggtaaac aggcatcagt ggcggatgcg ggaaacgctc atccagatat
     2521 tccataatga tgcgagattc ccacagggtc agctcacgat ccaccagggt cggaacgctc
     2581 tgattcgggt tgaggtcaat cagatcctga ggcggattgt ccttttccac gtgttcgatc
     2641 tcgaaactta cacctttctc agccagcaca atgcggacct gatggctata gatgtcagta
     2701 ggaccggaaa acagcgtcat taccgaacgt ttgttggcag cgacagccat gaaaacctcc
     2761 aggtatagtc agaattttta ctgctaccag ccaccaggtg gccagtcaga agttgtgtta
     2821 cccagtaagg aacgactctc tttgttcgaa aatcaaacaa aaaatgagca atacccgaca
     2881 tttgggcaga aaattggatg atagtttacc agattttgcg acctttgtgg tgagtcgatg
     2941 ccggaaatgg ggaaaaagag atgcgcttta gtctgaaata gttgacttag tcccttattg
     3001 gcgatgtggt ttttgttttg cctgtctgtc agacggtagc aaaaagcaac tttccaattt
     3061 ttacgctgat tcagaattta accataaaaa aacccgccga agcgggtttt ttcgcaaatt
     3121 gttttctgcc ggagcagaag ccaattaacg tttggagaac tgcggacgac gacgtgcttt
     3181 acgcagaccg actttcttac gttcaacctg acgagcgtca cgagtaacga agccagcttt
     3241 acgcagttca gaacgcaggg actcgtcgta ttccatcaga gcgcgggtga taccgtgacg
     3301 gatcgcacca gcctgaccag agataccacc acctttaacg gtgatgtaca ggtccagttt
     3361 ctcaaccatg tcgaccagtt ccagcggctg acgaactacc atgcgagcag tttcacgacc
     3421 gaagtactgt tccagagaac gttggttgat tacgatttta ccgttgcccg gtttgatgaa
     3481 aacgcgagct gcggaacttt tgcggcgacc agtgccgtag tattgatttt cagccattgc
     3541 ctataatccc gattagatgt caagaacttg cggttgctgt gccgcgtggt tgtgctcgtt
     3601 acccgcgtaa actttcagtt tacggaacat agcacgaccc agcgggcctt ttggcaacat
     3661 gcctttaacc gcgatttcaa tcacacgctc aggacggcga gcaatcatct cttcaaaggt
     3721 cgcttgtttg ataccaccga tgtggccggt gtggtgatag tacactttgt cagtacgctt
     3781 gttgccggtt acagcaactt tgtcagcgtt cagaacgatg atgtaatcac cggtatctac
     3841 gtgcggagtg tattccgctt tgtgcttacc gcgcaggcga cgagccagtt cagtagccag
     3901 acggcccaga gttttaccgg tcgcgtcaac aacataccag tcgcgtttta cggtttctgg
     3961 tttagctgta aaagttttca ttaaaagctt acccaataaa tagttacacg ttggtgaaca
     4021 cccaaacgtc ttcaattgtt gaggttcaca cgacaaagtc cggcaaacct accccttcga
     4081 atagcctatg ccagcacaca aaaagttttg ggaaaaaaac tttcttgtaa cgtggggtcg
     4141 caggattata gagaagtcgg ggtcaaagat cgaccccttt ttgtgatttg tgacaggttt
     4201 taacccgcca aatgctcgcg cttcagatac tcttcgcttt gcatctcttg cagacgtgac
     4261 aggcaacgct ggaactcaaa cttcagccga tcgccctgat aaatttcata cagcggcact
     4321 tctgcactca ccactaattt gacatggcgc tcgtaaaact catccaccag cgcaataaag
     4381 cgccgcgctt cgctctccat caaccgcgtc ataactggta catcaaacaa catgaccgta
     4441 tgaaagagac gtgagagcgc aatatagtca tgctgactgc gggcgtcgac gcacagcgta
     4501 gtaaaagaga ccgccagcgt ctggttctcg acgcccattg tcgccaatgg ccgatggttg
     4561 atttctaacg tcggtgaatt ttctcgtttc gcccccgcca gcgccaacca tagtttatcc
     4621 atctgcgccc gggtttcatc gttgagtggc gaaagccaca gatgcgcctg agtgagtgta
     4681 cgcagtcgat aatcaacacc agcgtccacg ttcattacat cacaatgctg tttaatggca
     4741 tcgattgcag gcagaaaacg cgcacgttgc aggccatttc gataaagttc atccggcgga
     4801 atatttgacg tcgctaccag ggtaatgccg cgggcgaaca gggctttcat cagaccgcca
     4861 agtagcatgg cgtcggtaat atcagaaaca aaaaattcgt caaaacagag cacgtcagtt
     4921 tcggctttaa agcgatcggc aataatttcc agcggatcgg tctgcccctg taaggcagtt
     4981 agctcttcgt gcacccgcag cataaaacgg tgaaagtgca ggcgctgttt ccgctctccc
     5041 ggcaggcttt gatagaaaag gtccatcagc caggttttcc cgcgtcctac accgccccac
     5101 atatataagc cacgcactgg cgtatgcttt gtgtcttcgc gtttacccca cagcttaccg
     5161 acccgcgcca ttagcccact cgtcctgggg gctggtggcc tgctattgat gagttcttga
     5221 taaataattt ccaggcggct gacggcctct ttttgaacgt cgtcgggttg atggctgcct
     5281 tcattgagcg ccttcaggta ttgcgatgtt ggggtaacgc tttgcatgat cttattgtta
     5341 ttccttgaat aatcggtgcg ccgttgttca cggttgacga aaaaaaggcc gttctacact
     5401 acgcgatatg cagtcgggat tccacttctg tggaattaac ggttatagtg gcataatcag
     5461 ccgcaggcat ggagcctgaa gccaacaccc tacggaaaca aaagacaacg ggagatgttc
     5521 atgacctggg aatatgcgct aattgggtta gtcgtcggca tcattattgg tgctgtggcc
     5581 atgcgttttg gtaatcgtaa actacgccag caacaggcgt tgcagtacga actggaaaag
     5641 aataaagctg aactggacga gtatcgcgaa gagctggtta gccactttgc ccgcagcgcg
     5701 gaattactgg ataccatggc gcacgactat cgccagctgt atcagcacat ggcgaaaagc
     5761 tccagcagcc tgctgccgga actgtctgct gaagcaaacc cgttccgtaa tcgtctggca
     5821 gagtctgaag ccagcaacga tcaggcaccg gtgcagatgc ctcgcgacta ttctgaaggt
     5881 gcatccggcc tgctgcgtac tggcgcgaag cgcgactaat ttatttttcg ggcgcagcca
     5941 ttgcgccctc ctcttctctc cctccccgac tatcatttaa tctggtgtct cattgttagc
     6001 cgtctgaaaa ttcaataaca tcaaactgtt ttgaatctct tttcttatca ttcaggtacg
     6061 agagcaggaa taatgaaaaa acaaacccag ctgttgagtg cattagcgtt aagtgtcggg
     6121 ttaactctct cggcgtcatt tcaggccgtc gcgtcgattc caggccaggt tgccgatcag
     6181 gcccctctcc ccagtctggc tccaatgctg gaaaaagtgc ttccggcagt ggtgagcgta
     6241 cgggtggaag gaacggccag tcagggacag aaaatcccgg aagaattcaa aaagtttttt
     6301 ggtgatgatt taccggatca acctgcacaa cccttcgaag gtttaggttc cggtgtcatc
     6361 atcaacgcca gtaaaggcta tgtgctgacc aacaaccatg tgattaatca ggcacagaaa
     6421 atcagtattc agctcaatga tgggcgcgag tttgatgcaa aactgattgg tagcgatgac
     6481 cagagcgata tcgccctgtt acaaattcaa aacccgagca aattaacgca aatcgctatt
     6541 gccgactccg ataaattgcg cgtcggtgat tttgccgtag cggtcggtaa cccatttggc
     6601 cttgggcaaa ccgccacctc tggcattgtt tccgcattag gccgcagcgg gttgaatctt
     6661 gaaggtctgg aaaactttat ccagacagat gcttccatta accgcggtaa ctccggcggt
     6721 gcactattaa accttaacgg tgagttaatt ggcatcaaca ctgcaatcct tgcgcctggc
     6781 ggcgggagcg tcgggattgg atttgccatc cccagtaata tggcgcgaac actggcgcag
     6841 cagcttatcg actttggtga aatcaaacgc ggtttgttag gcatcaaagg caccgagatg
     6901 agtgccgata tcgccaaagc cttcaacctt gacgtgcagc gtggcgcgtt tgtcagcgaa
     6961 gtgttgccag gttctggctc ggcaaaagcg ggcgtcaaag cgggcgatat tattaccagc
     7021 ctcaacggca aaccgctgaa tagctttgct gagttgcgct ctcgtatcgc gaccaccgag
     7081 ccgggcacga aagtgaagct tggcctgctg cgtaacggca agccactgga agtagaagtg
     7141 acgctcgata ccagcacctc ttcgtcggcc agcgctgaaa tgatcacgcc agcgctggaa
     7201 ggtgcaacgt tgagcgatgg tcagctaaaa gatggcggca aaggtattaa gatcgatgaa
     7261 gttgtcaaag gaagcccagc tgctcaggct ggcttgcaaa aagacgatgt gatcattggc
     7321 gtcaaccgcg atcgggtgaa ctcgattgct gaaatgcgta aagtgctggc ggcaaaaccg
     7381 gccatcatcg ccctgcaaat tgtacgcggc aatgaaagca tctatctgct gatgcgttaa
     7441 tgtcgtaaac cgggcatcag gcttacgtgt gatgtccggt taactcgtgg tatgctgctg
     7501 ccgttccctt ttttaatgac gcctccatca tgtttgtgaa gctcttacgt tccgttgcga
     7561 ttggattaat tgtcggcgct attctgctgg ttgccatgcc ttcgctgcgc agccttaacc
     7621 cgctttccac tccgcaattt gacagtaccg atgagacgcc tgccagttat aatctggcgg
     7681 ttcgccgcgc cgcgccagcg gtggttaacg tttacaaccg tggtttgaac accaactctc
     7741 acaaccagct tgagatccgc accctgggat ccggtgtaat catggatcaa cgcggttata
     7801 tcatcaccaa taaacacgtc atcaacgacg ccgatcagat catcgtcgcc ttacaggatg
     7861 gacgtgtatt tgaagcattg ctggtgggat ctgactctct aaccgatctg gcggtactta
     7921 aaattaatgc cactggcggt ttacctacca ttccgatcaa tgcacgtcgc gtaccgcaca
     7981 ttggcgacgt ggtactggcg atcggtaacc cgtacaacct cgggcagacc attacccagg
     8041 ggattattag tgctacgggt cgaatcggtc tgaacccgac cgggcggcaa aacttcctgc
     8101 aaaccgatgc ttccattaac cacggtaact ctggcggcgc gctggtgaac tcgctgggag
     8161 aactgatggg cattaacacg ctgtcgtttg ataagagtaa cgatggcgaa acgccggaag
     8221 gtatcggctt tgcgattcct ttccagttag caaccaaaat tatggataag ctgatccgcg
     8281 atggtcgcgt gatccgcggc tacattggta tcggcggacg cgagatcgca ccactgcacg
     8341 cgcagggcgg tggtatagat caactgcaag ggatcgtggt taatgaagtg tcacctgacg
     8401 gcccggcggc gaatgcgggt attcaggtca acgatctgat tatttcggtg gataacaaac
     8461 cggccatctc tgctctggag acgatggatc aggtggcaga aattcgccct ggttcggtga
     8521 tcccggttgt agtgatgcgt gatgataagc agttaacgct gcaggtcacc attcaggaat
     8581 atccggcaac caattaagtc gagtgctcaa aacaaaaaac cggagtctgt gctccggttt
     8641 tttattattc gctaattaat tacttattaa cgaactcttc gcccagggcg atatctttct
     8701 tcagcgtatc cagcatacct tccagtgcgc tctgttcaaa tgcgctcagg gtaccgatag
     8761 atttacgctc ttccacgccg tttttaccca gcagcagcgg ttgagagaag aaacgggcgt
     8821 actgaccgtc gccttcaacg taggcacatt cgacaacgcc ttgttcgccc tgcagtgcac
     8881 gaaccagaga cagaccaaaa cgtgcagctg cctggcccat agacagggtt gcagacccac
     8941 caccggcttt cgcttcaacc acttcagtac ctgcgttctg gatacgtttg gtcagatcag
     9001 ccacttcctg ctcggtaaaa ctaacgccag gaacctgtga cagcagcggc agaatggtaa
     9061 caccagagtg accgccaata accggcactt caacttcgcc tggctgtttg cctttcagtt
     9121 ccgcaacaaa agtgttggaa cgaatgatat ccagcgtggt aacgccgaac agtttgtttt
     9181 tgtcataaac accggctttt ttcagcactt cagcagcaat cgcaactgtg gtgttaaccg
     9241 ggttagtgat aataccaatg cacgctttcg ggcaggtttt cgcaacttgc tgtaccaggt
     9301 ttttcacgat gccggcgtta acgttaaaca ggtcggaacg atccataccc ggtttacgcg
     9361 ctacacctgc agagataaga acgacatctg cgccttccag cgccggagtc gcatcttcac
     9421 cagaaaaacc tttgattttc acagcagtag ggatatggct cagatcgaca gccacaccgg
     9481 gagtcactgg agcgatatca tacagagaga gttctgaacc tgaaggcagt tgggttttta
     9541 acagtagtgc aagcgcctgg ccaataccgc cagcagcgcc gaggactgcg actttcatcc
     9601 taaactcctt attatattga taaactaaga tatgttgctc cgctgccgcg accttaatcc
     9661 acaaaattgc cgtttacaat gaccacatct caagaatgtg tagtcacgca agtttagcgt
     9721 ttatgcattt aattgccgta atcaggaacc taacttacgt aattaacagt cgcttatgca
     9781 ttagcgcaac attccaacag gtggtgacaa tataccctac cgttcagcca aaacaacatc
     9841 aatttgataa caattaattt acttttaagc agaatttgca tgccgtgacg caggcatgtt
     9901 tctcaataac gaaatttgat aaaatcccgc tctttcataa cattatttca gccttcttca
     9961 gggctgactg tttgcataaa aattcatctg tatgcacaat aatgttgtat caaccaccat
    10021 atcgggtgac ttatgcgaag ctcggctaag caagaagaac tagttaaagc atttaaagca
    10081 ttacttaaag aagagaaatt tagctcccag ggcgaaatcg tcgccgcgtt gcaggagcaa
    10141 ggctttgaca atattaatca gtctaaagtc tcgcggatgt tgaccaagtt tggtgctgta
    10201 cgtacacgca atgccaaaat ggaaatggtt tactgcctgc cagctgaact gggtgtacca
    10261 actacctcca gtccattgaa gaatctggtg ctggatatcg actacaacga tgcagttgtc
    10321 gtgattcata ccagccctgg cgcggcgcag ttaattgctc gcctgctgga ctcactgggc
    10381 aaagcagaag gtattctggg caccatcgct ggcgatgaca ccatctttac tacccctgct
    10441 aacggtttca ccgtcaaaga cctgtacgaa gcgattttag agctgttcga ccaggagctt
    10501 taatctctgc cccgtcgctt ctggcggcgg ggaaaatgtt gcttatccct ctcattcccc
    10561 ttctttctcc tgcgatacat ttaacgaata gtgcgtttta ccgcgacaca tcattcacaa
    10621 aatgaataca
//
LOCUS       DAZGXZ010000164        10589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000164 DAZGXZ010000000
VERSION     DAZGXZ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.164"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..198)
                     /locus_tag="ACHKJF_004538"
     CDS             complement(<1..198)
                     /locus_tag="ACHKJF_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0951783.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWH"
     gene            complement(198..3224)
                     /locus_tag="ACHKJF_004539"
     CDS             complement(198..3224)
                     /locus_tag="ACHKJF_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951784.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASVVAQNTAAAKKSASDAGTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSVAATSAGAAKTSETNAAASEQSA
                     ATSASTAITKASEAATSARDAAASKEAAKSSETNASLSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSTTNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNALFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTTYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGLIPDMRSWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTH
                     SVTGASAVSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHT
                     HSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(3289..3888)
                     /locus_tag="ACHKJF_004540"
     CDS             complement(3289..3888)
                     /locus_tag="ACHKJF_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0951785.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMVG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(3955..7353)
                     /locus_tag="ACHKJF_004541"
     CDS             complement(3955..7353)
                     /locus_tag="ACHKJF_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951786.1"
                     /translation="MGKGGSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLNSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDLSGTVNGVTPPAVQHL
                     TVEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVESVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRICDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(7414..7986)
                     /locus_tag="ACHKJF_004542"
     CDS             complement(7414..7986)
                     /locus_tag="ACHKJF_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0951787.1"
                     /translation="MKTGAEAIRALATQLPVFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGIFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(7983..8726)
                     /locus_tag="ACHKJF_004543"
     CDS             complement(7983..8726)
                     /locus_tag="ACHKJF_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0951788.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            complement(8732..9430)
                     /locus_tag="ACHKJF_004544"
     CDS             complement(8732..9430)
                     /locus_tag="ACHKJF_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0951789.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGLELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(9430..9759)
                     /locus_tag="ACHKJF_004545"
     CDS             complement(9430..9759)
                     /locus_tag="ACHKJF_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0951790.1"
                     /translation="MKTFRWKVKPGMDVASSPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(9756..>10589)
                     /locus_tag="ACHKJF_004546"
     CDS             complement(9756..>10589)
                     /locus_tag="ACHKJF_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0951791.1"
                     /translation="DAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWA
                     AEDQLRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWR
                     SFTRSVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSLADSR
                     SQASGTFEQNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGG
                     R"
BASE COUNT         2114 a         3231 c         2705 g         2539 t
ORIGIN      
        1 atgccacgat gcctcatcac tgttgaaaac agccacaaag ccagcaggaa tatctggtgg
       61 tgcaatatcg gtactgtttg ctggcagacc tgtatgaggc ggaatatatg cgtcaccttc
      121 accaataaat tcattagttc cggccagcag attataaatt tttatggtcc gtgcttgttc
      181 actcattctg aatgccatta tgcaagcctc acaatatagt taaatgcgat gtttttgaca
      241 gtgttttccg cgttaccagc agcgttaacg gtgatggtgt gtccatgtga gccaatcgca
      301 accgagtgcg tatgagcacc aataccgaca gtatgtgcgt gtgcacctgc agatgcagcg
      361 gtgccgctga cactatgagt gtgcgctcct gcagcacttg tattcacact ggccgcagac
      421 actaccttat gtactgaccc attttgtgac cactggctga ctgctgatgc gcctgtgaca
      481 ctatgagtat ggtttccggc acttgcggct gtaccgctca cactgtgcgt atgcgccccg
      541 gtgttattcg tggatttagt gccgtaatca aacgacgatg tggttttcgt ccccaaatcc
      601 gtactggatg cgctggcgct gtgggtgtgc gatttaatgc cgtcctgttc ctgagacaat
      661 acggcccgac cactggcagg tttgccctta atcgtccagc tacgcatatc agggatcagg
      721 cctgacggat aagcggctgc aagtttcggg tatgcagatt tgtcaaaagt ctgcccctgc
      781 atcagggcat aaccagacgg aacggtatct gatggccacg ggattggtgc accgactgga
      841 taaaactctg caggaggatg agccgaggtg taaagctgcg cccacggcga ccagtttgcg
      901 tcggtcgtat cccgtcgtga acgaataaat gccggagcat gagcaccgct tgtaccactc
      961 cagccgatga gtaactcacc ttcgccaacg gctgtcatcc ctttcaggtg aatgatattt
     1021 ccatacgttg ttggatatcc gttgttatac acctcgtata actcaagacc tgctgccccc
     1081 tgcgtattgt ctgtcagcgc ggctacccga ccttttgaag ccagattaac tgatgatact
     1141 gctgttccac ctgacggtag cgccccgatc tctgatgccg ttggcttatt tctggagtta
     1201 tagtcccttc gccagccagg tgaataatct gttccgtgat taatataggt aaacagggcg
     1261 ttggttgttc cgccaccgct ggatgtggtc ggagtggtaa tgcggatcgt catcgctgac
     1321 tttatcccca ttacttcaat gacagctccg gcgagatgaa tattaccgca gccagtatca
     1381 gtaatgattt tattattgcc ataagaccag gaacccttgc acatccagta tggatggtta
     1441 aatgctccct gagaatccag ccactcgata aactgtgcag tcgtccagtt tcctgttgtt
     1501 gtgcttactg acccaccgaa ggcacggcag gcaccaatat ttttcgtaaa ggtgtctttg
     1561 ccagggatat ccgcaccgtt ctgatctttc tgcagacgtt tctcagcatt gtcattggct
     1621 gcttttactg cctttggcgt tgccgccagc gtttcagacg tgctgttggt cgtgctgctt
     1681 agttggacta tccctttctg tgctgtcgtt gcatcctgtg ctgtgtattt cccgttagcc
     1741 aggtcatacg cggccttaac tgcttttggc gttgccgcca gtgactcgga agtgctgttg
     1801 gtcgcactgc tgagctgtac tatccccttt ttcgtcgtgc tcgcatcctc aagcgccacg
     1861 gcggatgcaa tatcctctgc ccgttttgcc gctgtctcag cgcgcgttgc tgcagattcc
     1921 gccgtacttt tgctctgagc tgctgccgtc gcactaccag ctgcctctgt cgcctttgtg
     1981 gatgccgtcg tggcgctgcc cttcgctgct gacgcctgtc tggtcgcctc atcttttgaa
     2041 gcagacgccg atgatgccga tgacgccgcc gaactggcgg acgatgccgc tgccgttttt
     2101 gaggattctg cacgggtttc cgacgctttc gcgttcgttt cggatgtctt cgctgcggaa
     2161 gctgacctcg ctgctgccgt ggcctgctca gtggcttcgc cagccttcgt tgtggctgtt
     2221 gaagcggatg atgcggcgct ttctgccgat tttccggcgg cggtggcact ggctgaggcc
     2281 tgcccggcac ttgttgacgc ggcactggca gacgacgcag ccgctgtttt tgagcctgcc
     2341 gcagctgagg cgctctgtcc cgctgccgtc tcagaagacc tggcgttcgt ctcggacgtt
     2401 tttgccgcct tcgcggaatt tcctgccgcc gttgccgagg aagctgcgct actggcgctc
     2461 aaggatgcgt tcgtttctga tgatttcgct gcctcttttg aggccgccgc atcccgggct
     2521 gaggtggcag cttctgacgc tttcgtgatc gcggtggatg cagaagtggc ggctgattgt
     2581 tctgacgctg cggcattcgt ttctgacgtt ttcgccgcac cggcactggt agctgccacg
     2641 ctttttgagg actctgcagc ggcagcactt tttgaggctt cagtggcctt tgttgatgcc
     2701 gttcctgcgc tggaagacgc tgactgagcc gacgaagcgg cctgtccggc tgacgtgctg
     2761 gctgcacgtg ctgaatctgc agcatcagtc gcatgggttg ccgcctcacg ggcagatgtg
     2821 ccggcatcac tggctgactt cttcgcggct gccgtgttct gtgccaccac ggacgcgtta
     2881 cgcgctacct cttccaccat cagttcaaaa cggcgcagtg cctcaggacg ggcatcatcc
     2941 tccgtcatgg caccgagaaa atcattcagc gtaccgggtc gggaatcttc atacacggtg
     3001 atggtcccgg catgtgacgg cgggaatcct tccaccaaca gaataacgct gtactgaccg
     3061 tactcaacgt ccatgctgta acgcccggct tcatccggat tttctgaggc cagcgtgttc
     3121 accaccaccg tggtgctgtt acgttttgct ttcagctgga ttgtgcagtt ctgtaccggt
     3181 tttcctgtgc cgtctttcag tacacctgaa atctttactg ccatattcac cccacaaaaa
     3241 agcccgcctg aaccggcggg ctgtcataac actgtgttac ctggctaatc agaacttata
     3301 accgacaccc atgatgaaac cgtcagtgcg ccagtcgcca ctgccggagc cttcataagc
     3361 aatatcaatg gccacggatt cggtcgggtt aaactgcacg ccagctcccc acgccagaga
     3421 cgtgttgctg tggcgaccgt catcacttcc ggtcagcacg tcgtgcgttt tccccttgtt
     3481 gtcagttacg cggagataat ccccggagaa agtcgacaca cggctgtaag ccacacccgc
     3541 catcgcatac gcgctgaacc attcattcac gcgcacagac ggccccacca tcacgctgaa
     3601 ccagcggtta cgaacggaat cttcatgcca gcgggtatcg ctgtaacggg tcagctggcg
     3661 attcttgtct cctgcatagc tgaatgacgt caccagcccc agtgtgtccg taaactcata
     3721 acggtatttc acgttaatcc cgttcagatc atcgctgccg ggaacgttcg tcgaggcatg
     3781 aagatacccc gcgctcagcg tggactgatg ttcagacgcc catgcaggcg caccggatac
     3841 ggccagacaa atggctgcgg acaaaatggc ggcataaagt ttacgcataa ttacctctcg
     3901 cttttctgca ataaaaaagg cgtcatttct gacgcccgtt atgggttata aaattcagct
     3961 gatactgata cctgctgtgg atttcttcat caccacaacc agcagatcgc tgatactggt
     4021 tgttggtgtc cagttattcg ctcctgatga agatacggtg aatgtcagtg tcagtgtccc
     4081 ctgtccggca ggcatatcta taactgaaga aaatacgccc tgagcatccg tcgtggactg
     4141 attaaaaatc tcctgaccat tgcgggtcac tcttaaccgg caggttgaat accagtatga
     4201 ctgttggtta ttactgttga aattctcatg cttaccaccg cggaataaca ctggcggtat
     4261 catgacctgc cggtcaaatt tctgatcatc acagattctt accgtgatgg tgccgctggc
     4321 ataactgttc gtgcggggga aagacttgct gaccgttttg acaatatcgc cttcaatctg
     4381 gttggctgac agtttcccct taatctgaca gttctcatta atcgtgacgt tgttgagcgt
     4441 ccctgagttc gcattcacac tgccactgat atccgcattt ttagcggtca gctttccgtc
     4501 tgatgtcagg gaaaataccg gaggactgcc accgctggta atggtgggtg ccgtcaggcg
     4561 tttcaggaac acgtcgttca tgaatatctg gttgccctgc gccacaaaca tcggcgtttc
     4621 attcccgttt gccgggtcaa taaacgcgat acggttagcg gcaaccagaa actggctcag
     4681 tttgccttcc tccgtgtcct ccatactgag gccaataccc gcgacataat gtttgccgtc
     4741 tttggtctgc tcaattttga cagcccacat ggcattccat ttatcgttgg catccttcca
     4801 ctctttcgaa aactcatcca gtctgctggc gttatcctcc gtcagatcga ctttttccag
     4861 cagctcttta ccgagatggg attcggttat cttgcctttg aaaaaatcca ggtaaccttc
     4921 cgcatcatcg ctcgcccgac cgacggactc cacgaatgcc gatttgccaa cggtgttcac
     4981 actgcggata taaaagtaat aatcatggcc cggtttaata ttgatactgg cggctatcca
     5041 gtacagcccc gtgccaaggt agcgggctgt ggtttcaacc tgcctgatat cggtaatccg
     5101 cgtttccgag aaccagaact caaattgtac cgtcggatca taaaccgcaa gatgcggcgt
     5161 ggcggttatc tgaaaatatc ccggtgtcag ctcaatccga gacggcgctg ccggtgcggc
     5221 aatccggaac gataccgatg ccggatcgcc ctgctgtccc cacgcattta ccgcccggac
     5281 agtcagcctg tagttcccca gcgccagttg cgtgaagcgg tatgtggttt ccgtcgtcct
     5341 tgccgtactg accagccgct cactgccatc gtccgctgcc acggtcaggc gaagcaggaa
     5401 gctcaccccc ttcaccacct tcggcgtgtc ccagcgcgcc agcacctgat attccccgct
     5461 gtctgcggtg acttcgacag tcaggtgctg caccgcgggc ggcgtgacgc cattcaccgt
     5521 gccgctcaag tcgccgtcaa agtgcgcccc gttatccacg atggcttctt tttcgggtac
     5581 atgctgcacg gcggtaatag catacgtgcc gtcgtcgttc tcacggatac tcacgcagcg
     5641 gaacaggcgc tggcgcaacg tcggcaactt cagcccccat acgctgtatc cggcaacgcc
     5701 gtcaggaaca cggctcactt ttaccttcac gccgtcggtg acggactgga cctccacgct
     5761 gaccggattg ccacttccgt caaccaggct tatcagcgtg gtaccggagg atggcagcgt
     5821 gatttcacgg tcgagcgtca gcgtccgggt ctggctgttc accgccagca cgcgtccgcc
     5881 gatgctgatc cccgcatagt catcatcgca gatttcaata acatcacccg gcacatggcg
     5941 aagcccttcg gcacccacgc tgaaatccac ggtctgcgtc tccagcagct ccgttttaat
     6001 cagccacagc ccggcgcggt gtgcctgccc ccggctggta cagccaaagg catccatctt
     6061 cgtgacgtta cgaccgtaac ggagaatggc ctgcgtgtcc tccacaagct ctgtcgccgt
     6121 ctcccagccg ttatccgggt caatccagtt cacctcaacg gcattatggc ggtccttgag
     6181 ggcgctgaag ctgtagcgga acggcgcgcc atcatccggc atcaccacat tactgcggtt
     6241 ataggtccac accttatccg acggtcggtc ctgcacgaac gtcagcgtct gcccgttcca
     6301 taccggcata cagcgcatcg ccgagcagaa atcgctgaga acatcccacg ccttacgctg
     6361 tgtggtcagg taggcattac aggtgatgcg cggctccgtg ccgccaaagc cgtccggcac
     6421 tgactggtcg cagtactggc cgatgacata cagcgcccat ttatccacat ccgccgcacc
     6481 aagacgtttc cccatgccgt agcgcggatg ggtcagcata tcccacagac accaggccat
     6541 gttgttgctg tatgccggtt taaacgttcc gtcccagata ccgctgtatt gccgcgtctg
     6601 cgggttataa ttcgacggca cctgcagaat acgcccgcgc agatgataat tacggctcac
     6661 ctgctggctg ccgaactgct ccgagtccac ctgcacgccg accagtgccg tgttcgggta
     6721 gcactgtttc acatcgataa tttcggtgta tgacgaccag agcgttttgt tctgcagctg
     6781 gtctgtggtg ctgtccggcg tcatcctgcg catccggata ctgaacgggc gcggcggcag
     6841 gttatccacc accaccgagg ccagatactg tgaagtggtt ttacccttaa tggtgatgtc
     6901 cttttccgtc acccagccac cgttacgttg tatctgaacc agcaggcgga cttccgatgg
     6961 attcctgtcc ccctttgagg tggtttccac cagtgcctgc acgccgaaag taaaacgcag
     7021 acggtcaatg tttgccgacg tgatggtccg ggtgatcggc gtgtcatatt tcacttctgt
     7081 accgagcacc gtctcggagc cggaggattc aaatccctcc ggcggtgtct gctcctgctc
     7141 accggcccgg aacaccaccg tgacgccgga tatattggta ttcccctcac tgttcagcac
     7201 cggcgtactg ttcagcagca cgctttttaa tccatccacc ggaccttcaa ccggtccttc
     7261 gctgatggca tcgatcacac tcagcagctg cgtggacttc aggttgtcct tcgcttcgcg
     7321 cggggtatgc cccttactgc cgcctttacc cattcgtcat gctccataaa cgataaaacc
     7381 gcccggaggc ggtttcacat aaaacatttt gcatcagcga ccaatcacca caacctgacc
     7441 accatcccct tcgtctgccg tgctgatctc ctgagaaacc acgcgtgacc ccacgcgcat
     7501 ttcaccgtac agaaccggca gaacattgcc ctgggcaacc atgttatcca gtgaggagaa
     7561 ataggtgttc tgtttgccgt tatccgttgt ctgtgtacgg ggagttctgg ctttcggtgc
     7621 cagcatctgc gccacaccac cgagcaccat actggcaccg agagaaaaca ggatgccggt
     7681 catacctccg gccccaatgg ctgcccccca tgctgcaagg gtggctccgg cggtaaagaa
     7741 tgatccggca atggcggcag cccccaggac aatctggaat atgccacctg acttggcccc
     7801 ggcgactctg ggaacaatat gaatcacagc gccatcaggc agagtctcat gtaactgcgc
     7861 cgttaacccg gacgtgctga cgtcccgccc ggcaatccgt acctgatacc agccgtcgct
     7921 cagtttctga cgaaacaccg ggagctgtgt ggccagtgcg cggatggctt cagcccccgt
     7981 tttcacacga aggtcgatgc ggcggccaaa tcgttgcaaa tccccgtaaa ggcagatgcg
     8041 cgccatgccc gatgacgcca gagggagtgt gtgcgtcgct gccatttgtc ggtatacctc
     8101 tctcgtttgc tcagttgttc aggaatatgg tgcagcagct cgccatcacc acagtaaatg
     8161 gcggcatgat tcggcaccga tgaaccaaaa cagcacagca gcacatcgcc cggttgtgct
     8221 gatgacaacg gcacctgata cagccctgtg gcctccagat tatccagata gagattctga
     8281 ccgtgacgcc accagtcatc ctcgcgatga aaatccggca tctcaatccc cgccagatga
     8341 taagcatccc ggaacagcgt gtaacagtcc gtcaccccgt gctcaaagcg ccgcccggtg
     8401 agatgcggca cacagcggaa cttatgaatc gtcccccggc agaccagcca ccacggcaaa
     8461 tcactctgca cctgcagccg ccggtcggcc tcactcagcc agggcagacc accggggtgg
     8521 ctgtggacca gcgccacaat ctcaccctgc atttctgcct gcagccagtc ttccggcgac
     8581 atacggaaat acgcctccgg ctcaccggag atattcacgc aggggaaata tctttccccc
     8641 tccggcgtgc ttaccacgaa gccgcacgac tccgctggcg cacatcgccg ggcgtgcgcc
     8701 agaatcgctg attctgtctg tgtcatggga tttactgcga aagtttgtta atggaaagga
     8761 agccgccaaa gttgccgacg ttattgcgga acttacaacc gctcaggcat ttgctgcatt
     8821 tatccttcgt gatatcggac gtcggctggt catattcatc cgcgaccgcc ggaccgtgat
     8881 aaccgcactc atcgccgcga taggtccagg tgcaggtgtt ggccagcatg atacgtcccg
     8941 gaaaaacagc gccatccgtt tccgtcggcg tggacagtac aaaggaggca ctcaccgcgc
     9001 tcagttcgct gcactgctcg atgcgccagc ggctgatcac ctcctgctcc ggatcggcgt
     9061 cgctgtttcc gttgacgaag ttcaccgcat ccagaaaacg ggcgtaaacc ttacgccgga
     9121 ccaccgttcc gccgaccaaa ctctgcagat cttccgccat cccggtgacc ataccgtaca
     9181 ggttagaaac cgtcagcgtg gggcgcgtac tggtgccttt gccattcagt tcaagaccac
     9241 tcccctgaat gggatatggc tgatactgtc gcccctgcca ggtgaccggc tcaccttttt
     9301 cgttctgctc attacagaaa aaataacgtt ctccaccgac ctctgtcagg tcgatttccc
     9361 atagcaccac gctggccgac tgctccgcac gggtgcattc attcagtgtt tcctgccgga
     9421 tatcctgcat cagttcacca cctgttcaaa ctctgcgctg aactcaacac gcagcatact
     9481 gacccgcgac gaccattttg cgcaggtcac ctttatctgc cgccactcat aaggcggcgt
     9541 ccacagaaag gctttccagc ccccgtgctc ttccagaaac gactccagta ccgtggcctc
     9601 ctcacggggg acagaaagcg tcacgctgta cgttttcagg ttggcattca gcccggcagg
     9661 cgctcgctgg gaatagccat caccaaagcg cacctttctt acagaagggg acgaagccac
     9721 atccataccg ggtttcactt tccagcggaa ggttttcatc gtccacctcc ggagaacaga
     9781 ccaccatcgc gcatctgccc ggtcacaaca tccattgccg ccttacgggc tacgtcataa
     9841 acagccttca gtgcctgtgg ccctatctga ccgttcgtgc cgtcgttgtt aatcaccaca
     9901 tggttattct gctcaaacgt cccggacgcc tgcgaccggc tgtcagccag actgcccggt
     9961 gtaccgacat aaccaccggt ggcatagccg cgcatcagcc ggtagagatt ccccacgcca
    10021 atccggctgg ttgcctcctt cgtgaagaca aattcaccac ggtgaacaat ccccgctggc
    10081 tcatatttgc cgccggttcc tgtgaatcct ccggttgcaa aatggaattt cgccgcagcg
    10141 gcctgaatgg ctgtaccgcc tgacgcggat gcgccgccac caacagcccc gccaatggcg
    10201 ctgccgatac tcccgacaat ccccaccatt gcctgcttaa gcagaatttc tgtcatcatg
    10261 gacagcacgg aacgggtgaa gctgcgccag ttctgctcac tgccggtcag catcgctgcc
    10321 atattctgtg caataccatc aaaggtctgc gtggctgcac tttttacctg cgacatactg
    10381 tccgtggcgc tctcttccca ctcactccag ccggacttca ggcctgccat ccagctcccg
    10441 cgaagctggt cttcagccgc ccaggtcttt ttctgctctg acatgacgtt attcagcgcc
    10501 agcggattat cgccatactg ttccttcagg cgctgttccg tggcttcccg ttctgcctgc
    10561 cggtcagtca gcccccggct tttcgcatc
//
LOCUS       DAZGXZ010000165        10522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000165 DAZGXZ010000000
VERSION     DAZGXZ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.165"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            143..718
                     /gene="ssuE"
                     /locus_tag="ACHKJF_004547"
     CDS             143..718
                     /gene="ssuE"
                     /locus_tag="ACHKJF_004547"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP0951792.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            711..1670
                     /gene="ssuA"
                     /locus_tag="ACHKJF_004548"
     CDS             711..1670
                     /gene="ssuA"
                     /locus_tag="ACHKJF_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP0951793.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEIAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1667..2812
                     /gene="ssuD"
                     /locus_tag="ACHKJF_004549"
     CDS             1667..2812
                     /gene="ssuD"
                     /locus_tag="ACHKJF_004549"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP0951794.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2823..3614
                     /gene="ssuC"
                     /locus_tag="ACHKJF_004550"
     CDS             2823..3614
                     /gene="ssuC"
                     /locus_tag="ACHKJF_004550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP0951795.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3611..4378
                     /gene="ssuB"
                     /locus_tag="ACHKJF_004551"
     CDS             3611..4378
                     /gene="ssuB"
                     /locus_tag="ACHKJF_004551"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0951796.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4585..7197)
                     /gene="pepN"
                     /locus_tag="ACHKJF_004552"
     CDS             complement(4585..7197)
                     /gene="pepN"
                     /locus_tag="ACHKJF_004552"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP0951797.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7463..8665
                     /gene="pncB"
                     /locus_tag="ACHKJF_004553"
     CDS             7463..8665
                     /gene="pncB"
                     /locus_tag="ACHKJF_004553"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP0951798.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8834..10234
                     /gene="asnS"
                     /locus_tag="ACHKJF_004554"
     CDS             8834..10234
                     /gene="asnS"
                     /locus_tag="ACHKJF_004554"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP0951799.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2388 a         2713 c         2819 g         2602 t
ORIGIN      
        1 gatatataaa gccctttctt tagtttattt tcagaaaaag atacacaaaa tttatatttg
       61 gcattttctt gtctctccga cagactgacc gaattgttat caatgttaac aaaaaaagaa
      121 caattggtta taaggagaga gtatgcgtgt catcaccctg gcgggtagtc ctcgctttcc
      181 ttctcgctcc agctccttgc tggaatatgc gcgggaaaaa ctaaatggcc tcgatgtaga
      241 ggtttatcac tggaatctgc aaaacttcgc cccggaagat ctgctttatg ctcgtttcga
      301 tagtccggca ctcaagacct tcaccgaaca gctgcaacag gctgatggac tgatagtcgc
      361 tacacctgtg tataaagccg cctattccgg cgcgctgaaa accctgctcg acctgctgcc
      421 agagcgcgct ttgcaaggca aagtggtgct accgctggcg acgggcggta ccgtggccca
      481 tctgctggcg gtcgattatg cccttaaacc agttttaagc gcactgaaag ctcaggagat
      541 cctgcacggc gtgtttgccg atgactcaca agtaattgat taccatcaca gaccccagtt
      601 cacgccaaat ctgcaaaccc gtcttgatac cgcgctagaa actttctggc aggcattgca
      661 ccgccgcgat gttcaggttc ctgaccttct gtctctgcga ggtaatgccc atgcgtaaca
      721 tcattaaact ggcgctggtg ggattgctta gcgtctctac gattgcggtt gctgcagaat
      781 cctcgcctga agcgttacgt ataggctatc agaaaggcag tattggtatg gtactggcaa
      841 aaagccacca gttactggaa aaacgctatc cgcaatcaaa aatctcctgg gtggagttcc
      901 ccgcgggtcc gcaaatgttg gaagcgttaa acgttggcag tattgatctc ggcagtaccg
      961 gggatattcc gccaatcttt gcccaggctg ccggggctga tttggtatat gtgggcgtcg
     1021 agccaccgaa gcccaaagcc gaagtgattc tggtggcaga aaacagcccg atcaaaaccg
     1081 tagccgatct taaaggtcac aaagttgcct ttcagaaagg ttccagttca cacaaccttt
     1141 tactgcgtgc actgcgtcag gccggactta agtttaccga tatacaaccc acttacctga
     1201 cacctgccga tgcccgcgcc gcgttccagc aaggtaacgt tgacgcctgg gctatctggg
     1261 atccctacta ctccgctgcg ttattacagg gcggcgtgcg ggtgttgaaa gacggcaccg
     1321 atctcaatca aaccggatcg ttttatctgg cagctcgccc ctatgcagaa aaaaacggcg
     1381 cttttattca gggcgtactg gcaaccttta gtgaggccga tgcgttaacc cgcagccagc
     1441 gcgagcaaag catcgcttta ctggcaaaaa cgatgggctt accggcaccg gtgattgcct
     1501 cttacttaga tcatcgccct cctaccacca tcaaaccggt taacgccgag attgccgcct
     1561 tacagcagca aacggcagat ctgttttatg aaaatcgtct ggtgccgaaa aaagtcgata
     1621 ttcgccagcg catctggcag cccactcaac tggaaggaaa acaattatga gtctgaatat
     1681 gttctggttt ttaccgaccc acggtgacgg gcactatctg ggaacggaag aaggttcacg
     1741 cccggttgat cacggttatc tgcaacaaat tgcgcaagcg gcggatcgtc tgggctatac
     1801 cggtgtgctg atcccaacgg ggcgatcgtg tgaagatgcg tggctggtgg cggcgtcgat
     1861 gatcccggtg acgcagcggc tgaagtttct tgtcgccctg cgccccagcg taacctcacc
     1921 taccgttgcc gctcgccagg ccgccacgct tgaccgtctc tcaaatggtc gtgcgttgtt
     1981 taatctggtc acaggcagcg atccacaaga gctggcaggc gacggcgtgt tccttgatca
     2041 tagcgagcgc tacgaagcct cggcggaatt tacccaggtc tggcggcgct tattgctggg
     2101 ggaaaccgtc aatttcaacg gtaaacatat tcatgtgcgc ggagcaaaac tgctcttccc
     2161 gccgattcaa cagccttatc ctccgcttta ctttggcgga tcgtcagacg tcgcccagga
     2221 gctggcggca gaacaagttg atctctacct cacctggggc gaaccgccag aactagtaaa
     2281 agagaagatc gaacaagtgc gggcgaaagc tgccgcgcat ggacgcaaaa ttcgtttcgg
     2341 tattcgtctg catgtgattg ttcgtgaaac taacgacgaa gcgtggcagg ccgccgagcg
     2401 gttaatctcg catcttgatg atgaaactat cgccaaagca caggccgcat tcgcccggac
     2461 ggattccgta ggacaacagc gaatggcggc gttacataac ggcaagcgcg acaatctgga
     2521 gatcagcccc aatttatggg cgggcgttgg cttagtgcgc ggcggtgccg gaacggcgct
     2581 ggtgggcgat ggtcctacgg tcgctgcgcg aatcaacgaa tacgccgcgc ttggcatcga
     2641 cagctttgtg ctttcgggct atccgcatct ggaagaagcg tatcgggtcg gcgagttgct
     2701 gttcccacat ctggatgtcg ccattccgga aattccccag ccgcagccgc tgaatccgca
     2761 aggcgaagcg gtggcgaatg attttatccc ccgtaaagtc gcgcaaagct aaggaagaaa
     2821 taatggcaac gccagtgaag aagtggttat tgcgcgttgc cccctggttt ttaccggtgg
     2881 gcatcgtggc ggtgtggcaa ctggcctcct cagttggctg gctgtcgacg cgtattttgc
     2941 cctcaccgga aggggtggtg acggcgttct ggacgctctc cgccagcggc gaactgtggc
     3001 agcatctggc gatcagttcc tggcgggcgc tgattggctt ttcaattggc ggatcgctgg
     3061 ggttgatttt gggactgatt agcgggctat ctcgctgggg agaacggctg ctggatacct
     3121 caattcagat gttgcgcaac gtaccgcatc tggcgctgat cccgctggtg attttgtggt
     3181 ttggcattga tgaatccgca aaaatctttc tggtggcgct cggcacgcta ttccccattt
     3241 atatcaacac ctggcatggc atccgtaata tcgatcgcgg tctggtggaa atggcgcgta
     3301 gctatggatt atccggcata ccgctgttta tccatgtgat cctgcctggt gccctgcctt
     3361 caattatggt cggcgtgcgt tttgcgttgg gcctgatgtg gctgacgctg attgttgccg
     3421 aaaccatttc tgccaattca ggcattggtt atctggcgat gaatgcgcgg gagtttttgc
     3481 aaacggacgt ggtggtagtc gctattattc tttacgccct gctcggcaaa cttgctgacg
     3541 tcagcgcgca gctgttagag cgcctctggt tgcgctggaa cccggcttat catttgaagg
     3601 aggccacggt atgaatactg ctcgtctgaa ccagggcaca ccattgttgc tcaatgcagt
     3661 aagcaaacat tacgcggaaa atatcgtcct gaaccaactg gatttacata ttccggcagg
     3721 tcagtttgtg gcggtggtgg gccgcagcgg tggtggcaaa agtaccctgc tgcgcctgct
     3781 ggcaggtctg gaaacgccaa ccgcaggcga tgtgttagcg ggcaccacac cgctggctga
     3841 gattcaggaa gacacgcgaa tgatgtttca ggatgcgcgt ctgctgccgt ggaaatcggt
     3901 gattgataac gttgggttag gccttaaagg ccagtggcgc gatgccgcgc gtcgggcgtt
     3961 ggctgcagta ggactggaga atcgcgccgg ggaatggcct gccgcacttt ctggcgggca
     4021 gaaacagcgt gtggcgctgg caagagcgtt gattcatcga ccgggtttat tgttgcttga
     4081 tgaaccgctc ggggcgctgg acgccttaac gcgactcgag atgcaggatt tgattgtgtc
     4141 actttggcag cagcacggct ttaccgtact gttggtgacg catgatgtga gcgaagcggt
     4201 agcaatggct gaccgggtgc tgttaattga agagggaaaa attggtctgg atttgacggt
     4261 ggatatccct cgtccgcgcc ggttagggtc ggtgcgatta gcggagctgg aagcggaagt
     4321 attgcaacgg gtaatgcggc gtgggcattc tgaacaactg attcggcgtc atggttaata
     4381 tgccggatgt ggcgcacaaa accagcattt tcaacggact agtaggcctg ataagacacg
     4441 tcaagcatcg catcaggcag ttgggcacgg ctgccggatg cggcgtgaac gccttatcca
     4501 gcctacaaaa ccagcaattt caacggagtt gtaggcctga taagacgcgt caagcgtcgc
     4561 atcaggcatt ttggctcaaa agtatcaagc cagtgctttg gtgatcttct cgtacagatc
     4621 gccagagaga ttttccagcc ctttcaactg ttccagcgcc gcgcgcattt tctcctgacg
     4681 tttggcatca taacgtttca ggcgaatcag cggttcaatc agacgtgaag ctacctgcgg
     4741 gttacggctg ttgaggtcgg taagcatttc caccaggaac tggtaaccgc tgccatcttc
     4801 ggcatggaac gctgccgggt tgctgcccgc aaacgcacca atcaacgaac gaatacggtt
     4861 cgggttgctc atggtaaatg agcgatgctg caacagacca cgcaccgtct ccagcacatt
     4921 cgccgccggg ctggtggctt gcaggataaa ccatttatcc atcaccagac cgtcctgatg
     4981 ccacttatcg tcgtactcct gcatcagcgc gtcacggcaa ggcagctgtg cagcaaccgc
     5041 cgcagaaagc gctgccagcg catcggtcat attgtttgct tcatggtatt gcttgctcac
     5101 cagcacgtca gccagatgcg tttcaccaaa agcaaggaag cgcaggcagg cattacgcag
     5161 agtgcgtttt gcaatatctt catgctcaac acggtactcg ctctggtaat tcgcgttgta
     5221 aatagccagc agctcatccg ccagttcagt cgccagagta cgagtgagtg cttcgcgtac
     5281 ttcggcaata gcaatcgggt cgatgatatc gaacagttca gccatttcat tgacagaagg
     5341 cagcgtcagg atttctgccg ccagcgccgg atcaatcttc tcatcgagca gcaccgcgcg
     5401 gaaagcgtca gccacatgta ccggcagaga cagcggctgc ccttgctgat gacgggcgac
     5461 gttcagcttg atgtaggttg ccagcaaact ttgcgccgca tcccaacggg agaaatcatt
     5521 acgcgcatga cgcatcagga aggtcagttg ctgatcgctc cacttatatt ccagtttcac
     5581 tggcgcagag aattcgcaca gcagcgcagg caccggctgg aagtagacat tatcaaaaac
     5641 aaaggtctgt tccgcctggg tgacgttcag cacggaattc accggatgac cgcctttctg
     5701 caacgggatc actttgcctt cgttgtcata caattcgatg gcaaatggaa tatgcagcgg
     5761 ctgtttttct gcctgatccg gcgtggctgg cgtgcgctgg ctgatggtca gggtgtactg
     5821 ctcggtttcc ggattgtagt cgtctttgac ggtcacaatc ggcgtgccgg actggctgta
     5881 ccaacggcgg aaatgggaga gatcgacatt cgacgcatct tccatcgcct gcacaaagtc
     5941 gtcgcaggtt gccgcgctgc catcatggcg ctcgaaatag agttgcatcc ctttctggaa
     6001 gttttcttcg cccagcaggg tgtggatcat gcgaatcact tccgcgccct tctcgtaaac
     6061 ggtcagggtg tagaagttgt tcatctcaat gaccatatcc gggcggatcg ggtgcgccat
     6121 cgggctggcg tcttccgcaa actgcaatcc gcgcatggtg cgcacattat tgatacggtt
     6181 taccgcgcgg gaaccaaggt cagagctgaa ttcctgatcg cggaagacgg ttaaaccttc
     6241 tttcaggctg agctggaacc agtcgcggca ggtcactcgg ttaccggtcc agttatggaa
     6301 atattcatgg ccgataacgc gttcaatatc gagataatct ttgtcggtgg cggtgtcggt
     6361 gcgggccaac acatatttgg agttaaagat attcagaccc ttattctcca ttgcgcccat
     6421 attgaagaaa tccaccgcga cgatcatata gatgtcgagg tcatactcca ggccaaagcg
     6481 ttcttcatcc catttcatgg agtttttcag cgaggtcatc gcccacggcg cgcgatcaag
     6541 gttgccgcga tcgacgtaca gctccagtgc tacttcgcga ccagaacgcg tggtaaaggt
     6601 atcgcgcagt acatcaaagt cgcctgccac cagcgcaaac aggtagcacg gtttcgggaa
     6661 cgggtcctgc cactgtaccc aatggcgtcc gttttcgagt tcgccttgcg caacgcggtt
     6721 accattggaa agcaggaagg gatatttgat tttatcggca ataattttgg tggtaaaacg
     6781 cgccagcacg tccgggcggt cgagataata cgtaatatgg cggaaacctt cggcttcaca
     6841 ctgggtgcaa agcgcatcgc ctgactgata aagcccttcc agcgccgtat tcgccgccgg
     6901 gcttatttca ttaatgatct taagcgtaaa acgctccggc aaattactga tgaccagtgc
     6961 gccctcttct tctttccagg cggtccacgg ctcatcatta atatgaacag aaaccagttt
     7021 gaggtcttcg ccgttgagac gaagcggagc atctgatgca ccatgacgga cagcctggct
     7081 gaccgcggta acgaccgtct tttgcgcgtc gaggtcaaag gtcaagtcaa tatcagtaat
     7141 ctggtaatcc ggcgcacgat aatcgtgacg gtatttggct tgtggctgtt gagtcataaa
     7201 taacctttag catcttttat agagtctggt gttcagtcta ttcctgttgc gtaaatcgcg
     7261 ctatgcagaa tcttcatctt ttcaggtaca aacgccttta ttgctacatt tttataacat
     7321 acaccgcgta atgccatcga ccagaaaggt ggcatatggt gtgatcgggg ttcaataaat
     7381 tgcgaaacaa ggtatactcc agcagttcct gaagatgttt attgtactaa acgctcctgt
     7441 acgaggacgc tactgcgcac ctatgacaca attcgcttct cctgttctgc actcgttgct
     7501 ggatacagat gcttataagt tgcatatgca gcaagccgtg tttcatcact attacgatgt
     7561 gcatgtcgcg gcggagtttc gttgccgagg tgacgatctg ctgggtattt atgccgatgc
     7621 tattcgtgaa cagattcagg cgatgcagca cctgcgcctg caggatgatg aatatcagtg
     7681 gctctctgct ctgcctttct ttaaggccga ctatcttaac tggttacgcg agttccgctt
     7741 taacccggaa caagtcaccg tatccaacga taatggcaag ctggatattc gcttaagcgg
     7801 tccgtggcgt gaagtcatcc tctgggaagt tcctttgctg gcggttatca gtgaaatggt
     7861 acatcgctat cgctcaccgc aggccgacgt tgcgcaagcc ctcgacacgc tggaaagcaa
     7921 attagtcgac ttctcggcgt taaccgccgg ccttgatatg tcgcgcttcc atcttatgga
     7981 ttttggcacc cgtcgccgtt tttctcgcga agtacaagaa accatcgtta agcgtctgca
     8041 acaggaatcc tggttcgtgg gcaccagcaa ctacgatctg gcgcgtcggc tttccctcac
     8101 gccgatggga acacaggcac acgaatggtt ccaggcacat cagcaaatca gcccggatct
     8161 agccaacagc cagcgagctg cacttgctgc ctggctggaa gaatatcccg accaacttgg
     8221 cattgcatta accgactgca tcactatgga tgctttcctg cgtgatttcg gtgtcgagtt
     8281 cgccagtcgc tatcagggcc tgcgtcatga ctctggcgac ccggttgaat ggggtgaaaa
     8341 agccattgca cattatgaaa agctgggaat tgatccacag agtaaaactc tggttttctc
     8401 tgacaatctg gatttacgca aagcggttga gctataccgc cacttctctt cccgcgtgca
     8461 attaagtttt ggtattggga ctcgcctgac ctgcgatatc ccccaggtaa aacccctgaa
     8521 tattgtgatt aagttggttg agtgtaacgg taaaccggtg gcgaaacttt ctgacagccc
     8581 tggcaaaact atctgccacg ataaagcgtt tgttcgggcg ctgcgcaaag cgttcgacct
     8641 tccgcatatt aaaaaagcca gttaatatca tcagggagct aatcggctcc ctttttttac
     8701 ctttaattcc gaaatctttc gctgcatttg cgaattctgc ttgtctgatt gcagatgcca
     8761 ggtaacatag gtatcccccc atttacgggt ggacaagtgt ttattttttc cgactattaa
     8821 cagagagaat attatgagcg ttgtgcctgt agccgacgta ctccagggcc gcgtagccgt
     8881 tgacagcgaa gtcaccgtac gcggatgggt acgtacccgc cgagattcaa aagctggcat
     8941 ctccttcctc gccgtttatg acggttcctg ctttgatcct gtacaggctg tcatcaataa
     9001 ttctctgccc aattacaatg aagacgtcct gcgtctgacc accggctgct cggtcattgt
     9061 gacgggtaaa gtcgtggcgt cgccgggcca ggggcaacaa tttgaaattc aggccagcaa
     9121 agttgaagtt gctggttggg ttgaagatcc agacacttac ccgatggcgg caaaacgcca
     9181 cagcattgag tatctgcgtg aagtcgctca cctgcgtccg cgcacaaacc tgattggtgc
     9241 cgtcgcgcgc gttcgccata cgctggcgca ggcgctgcat cgcttcttta acgagcaggg
     9301 attcttctgg gtttcaacgc cactgattac cgcatctgat accgaaggtg caggcgaaat
     9361 gttccgcgtt tctacgctgg atctggaaaa cctgccgcgt aacgatcagg gcaaagtgga
     9421 tttcgacaaa gacttctttg gtaaagagtc tttcctgacc gtatctggcc agttgaacgg
     9481 cgaaacctac gcttgcgcat tatccaaaat ttataccttc ggcccgactt tccgtgctga
     9541 aaactccaac accagccgtc acctggcgga attctggatg ctggagccgg aagtggcgtt
     9601 tgctaacctg aacgatatcg cgggtctggc tgaagccatg ctgaaatatg tcttcaaagc
     9661 ggttctcgaa gaacgcgcag acgacatgaa attcttcgct gaacgcgtag ataaagatgc
     9721 cgtttcacgt ctggaacgct tcattgaggc cgatttcgcg caggtggatt acaccgacgc
     9781 agtgaccatt ctcgaaaact gcggcaggaa gtttgaaaac ccggtttact ggggcgtcga
     9841 tctctcttct gagcatgagc gttatctggc ggaagaacac tttaaagcac cggtagtggt
     9901 taaaaactat ccgaaagata ttaaagcgtt ctatatgcgc ctcaacgaag acggtaaaac
     9961 cgttgcggct atggacgttc tggctccggg catcggtgag atcattggtg gctcccagcg
    10021 tgaagaacgt ctggacgttc tggacgagcg tatgctggaa atgggcctga ataaagaaga
    10081 ttactggtgg tatcgcgatc tgcgtcgcta cggtactgtt ccgcattcag gtttcggtct
    10141 tggttttgaa cgcctgattg cttacgtaac tggcgtgcaa aacgtgcgtg atgtgattcc
    10201 gttcccacgt actccgcgca acgccagctt ctgattttct tcctcaaaga gccagcgcct
    10261 gctggctctt ttttatcctt tctgtcaagt tatctgtttg ttaagtcaag caatctattt
    10321 gcaaccccgc cataacgatc atcctgttac ggaatattac attgcaacat ttacgcacaa
    10381 aaaataaccc gcattcttat tgcggattag ttttttctta gctgatagca caattttcat
    10441 actatttttt ggcattctgg atgtctgaaa gaggattttg tgccaggtcg ataaagtttc
    10501 catcagaaac aaaatttccg tt
//
LOCUS       DAZGXZ010000166        10230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000166 DAZGXZ010000000
VERSION     DAZGXZ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.166"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHKJF_004555"
     CDS             complement(114..2687)
                     /gene="clpB"
                     /locus_tag="ACHKJF_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP0951800.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHKJF_004556"
     CDS             complement(2817..3548)
                     /gene="yfiH"
                     /locus_tag="ACHKJF_004556"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP0951801.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHKJF_004557"
     CDS             complement(3545..4525)
                     /gene="rluD"
                     /locus_tag="ACHKJF_004557"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP0951802.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4660..5397
                     /gene="bamD"
                     /locus_tag="ACHKJF_004558"
     CDS             4660..5397
                     /gene="bamD"
                     /locus_tag="ACHKJF_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP0951803.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5668..6009
                     /gene="raiA"
                     /locus_tag="ACHKJF_004559"
     CDS             5668..6009
                     /gene="raiA"
                     /locus_tag="ACHKJF_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP0951804.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6112..6237
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6113..6160
                     /gene="pheL"
                     /locus_tag="ACHKJF_004560"
     CDS             6113..6160
                     /gene="pheL"
                     /locus_tag="ACHKJF_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP0951805.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6259..7419
                     /gene="pheA"
                     /locus_tag="ACHKJF_004561"
     CDS             6259..7419
                     /gene="pheA"
                     /locus_tag="ACHKJF_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP0951806.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHKJF_004562"
     CDS             complement(7462..8583)
                     /gene="tyrA"
                     /locus_tag="ACHKJF_004562"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP0951807.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRMMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHKJF_004563"
     CDS             complement(8594..9664)
                     /gene="aroF"
                     /locus_tag="ACHKJF_004563"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP0951808.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9874..>10230
                     /gene="yfiL"
                     /locus_tag="ACHKJF_004564"
     CDS             9874..>10230
                     /gene="yfiL"
                     /locus_tag="ACHKJF_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP0951809.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIR"
BASE COUNT         2628 a         2712 c         2394 g         2496 t
ORIGIN      
        1 gaaaaatata aaaactttat cgttcgctca tttttcaggc aaaacatctt aaatatagtc
       61 ttttccgtct aacttataga caaaaacgag ccccgaaggg ctcgttttat catttactgg
      121 acggcgacaa tccggtcttc attaacttcc aggcgaatca ctttacccgg aaccaattca
      181 ccagacagta tttgctgtgc cagcgggttt tcgatctgct gctggatagc acgtttcaaa
      241 ggacgtgcac catagactgg atcgtaaccg ttctcgctca gcagtttcag cgcctcgtca
      301 gaaatgtgga tttcataacc acgttcttcc agacgtttgt acagacgttt caactgaatc
      361 tgcgcaatcg aggcaatgtg ctgttcaccc agcggatgga agaccaccac ttcatcaata
      421 cggttaatga attccggacg gaagttatgg cttaccacac cgagcaccag ctctttcatg
      481 tgcgcataat ccagttcacc gaagcgttcc tgaatcaaat cggaaccgag gttagaggtc
      541 ataatgacaa ccgtattacg gaagtcgacc gttctcccct gcccgtcagt cagacgacca
      601 tcatccagta cctgcaacag aatgttgaag acatccggat gcgctttttc cacttcatcc
      661 agcaggatga cggaatacgg acgacgacgc acagcttcgg tcaggtagcc accttcttca
      721 taaccgacat atcccggagg cgcaccaacc agacgagaca ccgagtgttt ctccataaac
      781 tcggacatat cgatacggac catcgcctcg tcgctatcaa acataaagtt cgccagcgcc
      841 ttacaaagct cagttttccc cacacccgtt gggccgagga acaggaatga accaatcggg
      901 cgatttggat ccgccaaccc cgcacggcta cggcgaatag cgttagatac cgcatcaacc
      961 gcttcgttct gaccaatgac gcgatggtgc agttcttgct ccatacgcag cagtttttcg
     1021 cgctcgcttt ccatcatgcg agaaaccgga atccccgtcc aacgcgccag cacttcagca
     1081 atttcggcgt cggtcacttt attacgcaac agacgcatag ttttgccttc gagctgcgtt
     1141 gcggcttcca gttgcttttc cagttccggg attttgccgt attgcagttc agacatccgc
     1201 gccaggtccc ccacacggcg agcctgttca atagcgattt tcgcctgttc cagttccgct
     1261 ttaatggtct gcgtaccaga aagcgatgcc ttctctgctt tccactcttc ttctaactcg
     1321 gagtactgac gttctttgtc gctcagttct tcgttgagca tatccaggcg ttttttactg
     1381 gcttcatcag actctttcat taacgcctgt tgttccagtt tgagctggat gatacgacga
     1441 tcgagtcggt cgagttcttc tggttttgag tcaatctgca tacgaatgct ggatgctgct
     1501 tcatcgatca ggtcgatggc tttatccggc agctgacggt cagcaatgta gcgatgagac
     1561 aacgtcgccg ctgcaacaat tgccgggtca gtaatttgca catggtggtg caattcgtaa
     1621 cgttctttca ggccacgcag aatcgcaatg gtatcttcaa cagaaggctc ggcaacaaac
     1681 actttctgga aacgacgttc cagcgcagca tctttttcga tgtactggcg atattcgtca
     1741 agcgtcgtgg cacctacgca gtgcaattca ccacgcgcca gcgccggttt cagcatgttt
     1801 ccggcgtcca ttgcgccatc ggctttaccc gcgccgacca tggtatgtaa ttcgtcgata
     1861 aacaggatga cgttgccttc ctgtttggca agatcgttaa gcacgccttt taaacgctct
     1921 tcaaactcac cgcgatattt cgccccagcc accagcgcgc ccatatccag cgccagtacg
     1981 cggcggcctt tcaatccttc cggcacttcg ccgttgataa tacgctgcgc cagaccttca
     2041 acgatggcag ttttaccgac gccgggttca ccaatcagta ccgggttatt tttagtacga
     2101 cgttgcagca cctgaatggt acggcgaatt tcttcatcac gaccaatcac cggatcgagt
     2161 ttgccctgtt cggctcgttc ggtaaggtcg atggtatatt ttttcaaagc ctgacgttgg
     2221 tcttcagcac cttgatcgtt cacgctttca cctccacgca tttgttcaat cgcttgagta
     2281 atgttggcgg tggtcgcccc tgctgctttc aggatgtcgg ccagcgtgcc gcgagactca
     2341 agtgccgcca gaacgaacag ttctgacgag ataaagttat caccacgttt ttgcgccagc
     2401 ttgtcgcaaa gattaagaac gcgcaccaga tcctgtgatg gctggacatc accaccagta
     2461 ccttcaacct gcggtaaacg atttaatgcc tgattgatat ctgtgcgcaa ctggccagca
     2521 tttatgccag cggatgttaa taaaggacta accgaacccc cttcctgatt cagcagggcg
     2581 ctcattaaat gaagtggttc gataaattgg ttgtcgtgcc cgagtgcaag tgattgggca
     2641 tcggcaagag caagctggaa tttattagta agacgatcca gacgcataac tcctcccata
     2701 acggatcaaa gttgctactg gagattaaat gaggtcatcc ctcaattatt caaggttatt
     2761 gaccagatta atgtgaaaag aaaatcacgc gtaccggatc gtcttgattc tttaggttat
     2821 atcagccaaa tgaaacttgc catacgaccg gtggtcttgt cgcgacgata agagaagaaa
     2881 gtctcatttt ccgtatatgt acaacggtcg ccgccgaaaa tttgctcaac acccacgttc
     2941 gccagacgct gccgggcaag ctgataaata tccgccagat acttatcacc atgctgaatg
     3001 aaagctgcac ttgctttagc gtctactgcc ataaacgcct cgcgaacctc cacccccact
     3061 tcgaacgcgc gtggaccaat tgccggccct aaccaggcga gaatattttc cggattatca
     3121 gcaaaacagg aaaccgtctc ttccagcacg cctgcgcaca gtccacgcca gccagcatga
     3181 gcggcggcga cttccgttcc cgctcgattg caaaacagca cagggaggca gtcggcagtc
     3241 atcactgcgc aaaccgtgcc gggcgtattg ctataagagg catctgctcg ttttgaggca
     3301 taaggttcgc cagtaagctt aagcacatct ttgccgtgta cctgctcaag ccagaccggt
     3361 ttagaaggca aattgcccgc agcaaaaagt cgcttgcgat tctcctcaac gtgatccggg
     3421 ttatcgccac aatgggcacc gaggttgagc gagtcatacg ggggcaagct cacgccgccg
     3481 atacgagaag agctacaggc cgcaacacct tttggtagcg gccactgcgg gacaatcagc
     3541 ttactcataa ccagtccact tcatccttat gctcttcgaa atcggcgcgc atcacctcaa
     3601 tcagctccac catatcttgt ggaataggcg catgccattc catttcgatg ccggagatcg
     3661 ggtgataaag acgcagcatg gttgcatgaa gcgcctggcg gtcaaactta cgcagcatgg
     3721 agataaatgc ttccgaagca ccttttggcg gacgcggacg gccaccataa accggatcgc
     3781 ccaccagcgg atgagtgata tgggccatat gcacgcggat ctggtgcgta cgtccagttt
     3841 ccagacgcaa ccgcagacgc gtgtgcacac ggaagtgttc catgatgcga tagtgagtca
     3901 ccgccggttt gcccatcgga tgcaccgcca tgtgagtacg tttggtcggg tggcggctga
     3961 tgggttcatc tactgtaccg ccagcggtca tatgaccaat cgccaccgct tcatactcac
     4021 gagtaatttc acgccgttgc aaagattcga ctaaacgcgt ctgagccgga acggtttttg
     4081 ccacaaccat caggccagtg gtgtctttat ccagacgatg gacgatgccc gcacgtggta
     4141 catcggcaat gggcggatag taatgaagca acgcattcag taccgtgcca tccgggttac
     4201 ccgcgccagg atgtaccacc aggtcgcgcg gtttattaat gacgatgata tcttcatctt
     4261 catagacgat atccagcggg atatcctgcg gttcaaaacg cgcttcttct tcaatctcag
     4321 cattgatggc aacctgctcg ccacccaata ctttttcttt cggcttatca caaactttgc
     4381 cgtttaccag cacgcgctgg tcgaggatcc attcttttat tcgcgaacgt gaataatccg
     4441 ggaacatttc ggccaaagcc tgatctaagc gttgaccgag ttggttttcg gacaccgttg
     4501 cagtgagctg tactcgttgt gccatatata ctgcttcttc gtttatcgtt ggggttttac
     4561 ggctttgccg tttaatatag tgtgctattg tagctggtct taaccgggag caggaacaga
     4621 gaatctcccg tattacattt tgaggaaagt caaaacgtca tgacgcgcat gaaatatctg
     4681 gtggcagccg ccacactaag cctgtttttg gcgggttgct cggggtcaaa ggaagaagta
     4741 cctgataatc cgccaaatga aatttacgcg actgcacaac aaaagctgca ggacggtaac
     4801 tggagacagg caataacgca actggaagcg ttagataatc gctatccgtt tggtccgtat
     4861 tcgcagcagg tgcagctgga tctcatctac gcctactata aaaacgccga tttgccgtta
     4921 gcgcaggctg ccatcgatcg ttttattcgc cttaacccga cccatccgaa tatcgattat
     4981 gtcatgtaca tgcgtggcct gaccaatatg gcgctggatg acagtgcgct gcaagggttc
     5041 tttggcgttg accgtagcga tcgcgatcct caacatgcac gagctgcgtt tagtgacttt
     5101 tccaaactgg tgcgcggcta tccaaacagt cagtacacca ccgatgccac caaacgtctg
     5161 gtattcctga aagatcgtct ggcgaaatat gaatactccg tggccgagta ctatacagaa
     5221 cgtggcgcat gggttgccgt cgttaaccgc gtagaaggaa tgttgcgcga ctacccggat
     5281 acccaggcta cgcgtgatgc gctgccgctg atggaaaatg cataccgtca gatgcagatg
     5341 aatgcgcaag ctgaaaaagt agcgaaaatc atcgccgcaa acagcagcaa tacataacag
     5401 aaacctgaaa tacaaaacgg cagcccttga gctgccgttt ttttattctg ttagttgtga
     5461 aactgaagcg atttagtcac tatcgatctc atcaaatatg gctcgctttg agatattcct
     5521 caagtaaaaa aacacctctt cctgcgattt ctcacaaaaa agattcgttg acaaaaagtg
     5581 acaaaattat gagattccca tcacacattt tgacatcagg agcggtatgc tgaattcacc
     5641 aagacgggaa gacaagaggt aaaatttatg acaatgaaca ttaccagcaa acaaatggaa
     5701 attactccgg ccatccgcca acatgtcgca gaccgtctcg ccaaactgga aaaatggcaa
     5761 acacatctga ttaatccaca tatcattctg tccaaagagc cacaagggtt tgttgctgac
     5821 gccacaatca atacacctaa cggcgttctg gttgccagtg gtaaacatga agatatgtac
     5881 actgcaatta acgaattgat caacaagctg gaacggcagc tcaataaact gcagcacaaa
     5941 ggcgaagcac gtcgtgccgc aacatcggtg aaagacgcca acttcgtcga agaagttgaa
     6001 gaagagtagt cctttatatt gagtgtatcg ccaacgcgcc ttcgggcgcg ttttttgttg
     6061 acagcgtgaa aacagtacgg gtactgtact aaagtcactt aaggaaacaa acatgaaaca
     6121 cacaccgttt ttcttcgcat tcttttttac cttcccctga atgggaggcg tttcgtcgtg
     6181 tgaaacagaa tgcgaagacg aacaataagg cctcccaaat cggggggcct tttttattga
     6241 taacaaaaag gcaacactat gacatcggaa aacccgttac tggcgctgcg agagaaaatc
     6301 agcgcgctgg atgaaaaatt attagcatta ctggcagagc ggcgcgaatt ggccgtcgag
     6361 gtaggaaaag ccaaactgct ctcgcatcgc ccggtacgtg atattgatcg cgaacgcgat
     6421 ttactggaaa gattaattac gctcggtaaa gcgcaccatc tggacgccca ttacattact
     6481 cgcctgttcc agctcatcat tgaagattcc gtattaactc agcaggcttt gctccaacaa
     6541 catctcaata aaattaatcc gcactcagca cgcatcgctt ttctcggccc caaaggttct
     6601 tattcccatc ttgcggcgcg ccagtatgct gcccgtcact ttgagcaatt cattgaaagt
     6661 ggctgcgcca aatttgccga tatttttaat caggtggaaa ccggccaggc cgactatgcc
     6721 gtcgtaccga ttgaaaatac cagctccggt gccataaacg acgtttacga tctgctgcaa
     6781 cataccagct tgtcgattgt tggcgagatg acgttaacta tcgaccattg tttgttggtc
     6841 tccggcacta ctgatttatc caccatcaat acggtctaca gccatccgca gccattccag
     6901 caatgcagca aattccttaa tcgttatcca cactggaaga ttgaatatac cgaaagtacg
     6961 tctgcggcaa tggaaaaggt tgcacaggca aaatcaccgc atgttgctgc gttgggaagc
     7021 gaagctggcg gcactttgta cggtttgcag gtactggagc gtattgaagc aaatcagcga
     7081 caaaacttca cccgatttgt ggtgttggcg cgtaaagcca ttaacgtgtc tgatcaggtt
     7141 ccggcgaaaa ccacgttgtt aatggcgacc gggcaacaag ccggtgcgct ggttgaagcg
     7201 ttgctggtac tgcgcaacca caatctgatt atgacccgtc tggaatcacg cccgattcac
     7261 ggtaatccat gggaagagat gttttatctg gatattcagg ccaatcttga atcagcggaa
     7321 atgcaaaaag cattgaaaga gttaggggaa atcacccgtt caatgaaggt attgggctgt
     7381 tacccaagtg agaacgtagt gcctgttgat ccaacctgat gaaaaggtgc cggatgatgc
     7441 gaatcatccg gcactggatt attactggcg attgtcattc gcctgacgca ataacacgcg
     7501 gctttcactc tgaaaacgct gtgcgtaatc gccgaaccag tgctccacct tgcggaaact
     7561 gtcaataaac gcctgcttat cgccctgctc cagcaactca atcgcctcgc cgaaacgctt
     7621 atagtaacgt ttgattaacg ccagattacg ctctgacgac ataatgatgt cggcataaag
     7681 ctgcggatcc tgagcaaaca gtcgcccgac catcgccagc tcaaggcggt aaatcggcga
     7741 agagagcgcc agaagttgct caagctgaac attttcttct gccaggtgca gcccgtaagc
     7801 aaaagtagca aagtggcgca gagcctgaat aaacgccata ttctgatcgt gctcgacagc
     7861 gctaatacga tgcagccgag cgccccagac ctgaatttgc tccagaaacc attggtatgc
     7921 ttccggctta cgtccatcac accagaccac aacttgcttt gccaggctac cgctgtccgg
     7981 gccgaacatc gggtgtaacc ccagtaccgg gccatcgtgc gccgccagca tggcctgtaa
     8041 tggtccattt ttcactgatg ccagatcaac cagaatacaa tctttcggta aaggcggtaa
     8101 tttgccgata acttgctcag taacgtggat tggcacacta acaatcacca ttccggcatc
     8161 ggcaacaata tcagccgctc gatcccagtc atgttgctcc agaatccgca cctgataacc
     8221 cgatagtgtc agcatcttct cgaacaggcg tcccatctga ccgccaccgc cgacgataac
     8281 caccggacgc agtgacggac aaagtgtttt aaatcctttg tcgttttcac tggagtaaga
     8341 ttcacgcatc atccgacgca aaacatcctc aatcagatct ggcggtacac ccagagcttc
     8401 cgcctctgca cgacgcgagg ccaacataga tgcctcgcgc tccggaacat aaataggcag
     8461 tccaaagcgg cttttcacct cgcccacttc agcaaccagt tccagacgct tcgctaataa
     8521 attcagcagc gctttatcga cttcatcaat ttgatcgcgt aatgcggtca attcagcaac
     8581 cataataaac ctcttaagcc acgcgagccg tcagctgccc gttcagatcc tgatgaattt
     8641 cacgcagcaa ggcatcggtc atttcccagc taatgcaggc atcggttacg gatacaccgt
     8701 atttcatttc actgcgcggt tgctcggaag actgattgcc ctcgtggata ttactttcga
     8761 tcatcagacc aataattgag cgattgccat ctttgatttg agcaaccacg gattctgcca
     8821 ccgcaggctg acggcgataa tctttattgg aattaccgtg gctgcaatct accatcagag
     8881 acgggcgcag tcccgcctgt tccatctctt tttcacattg cgcaacatcc gcagggctat
     8941 agttcggcgc tttaccaccg cgcaggatca catggccgtc cggattcccc tgagtttgta
     9001 gcaacgcaac ctgccctgcc tggttaatgc caacaaaacg gtgcggctgg gcggcggcgc
     9061 gcatagcgtt aattgctgtt gccagactgc cgtcggtgcc gtttttaaaa ccaaccggca
     9121 tggaaagccc ggaggccatt tcacggtgag tttgcgattc cgttgtacga gcaccaattg
     9181 ctgaccagct aaacagatcg cccaggtatt gcgggctatt cggatctaac gcttccgtcg
     9241 ccagtggcag tcccatattc accagctcaa gcagcaattt acgcgcgatc tgcagcccgg
     9301 cttctacatc aaaagagcca tccatatggg gatcgttaat taaccctttc cagccgacag
     9361 tggtacgggg tttttcaaaa tagacgcgca ttaccagata gaggctatcg ctgacctctg
     9421 cggcaagggc tttaaatcga cgagcatatt ccagagcagt ttccggatca tgaatggaac
     9481 aaggaccaca tactaccagc agacgaggat cgcgcccggc gataatatct gaaatggttc
     9541 tacgcgagtc agcaatctgg gcttcttgtt gcaggctcaa tggaaaagcg gccttcagtt
     9601 gttccggagt cattaaaacc tgttcgtcgg taatatgtac gttattcagc gcgtcttttt
     9661 gcatgatggc gatcctgttt atgctcgttt gcgatagttg atcctcagcg aggatgacgt
     9721 aacgataaca cataaagtaa agttttcaat ccatatttcg tacattttta tttacacagg
     9781 caatttagtc gcgatttcaa cccttacttc tgtatagata aatttacact ccctttgaaa
     9841 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
     9901 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
     9961 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
    10021 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
    10081 ggacaaaaga aaacttgcca gaccgatttt acttatgccc gaggactttc cggtaaaagc
    10141 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
    10201 ggggctgatg aaaatgccag cacaatacga
//
LOCUS       DAZGXZ010000167        10225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000167 DAZGXZ010000000
VERSION     DAZGXZ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.167"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..622)
                     /locus_tag="ACHKJF_004565"
                     /pseudo
     CDS             complement(1..622)
                     /locus_tag="ACHKJF_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            914..1465
                     /locus_tag="ACHKJF_004566"
     CDS             914..1465
                     /locus_tag="ACHKJF_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0951810.1"
                     /translation="MRKYITNSEWALFFNAITGTRNEIRDKALLQITYIHGLRVSELI
                     DLRLGDFDMTEKIVFIRRLKNGLSTSHPLQPETIELLQRWFLIRKRYTKGSQDDYLFL
                     SSHGKKISRQWIYKLCARYSQRAGLRIKVHPHMLRHACGYALANQGLDTRLIQDYLGH
                     RNIHHTVLYTASNPGRFNRVWRS"
     gene            complement(1462..2304)
                     /locus_tag="ACHKJF_004567"
     CDS             complement(1462..2304)
                     /locus_tag="ACHKJF_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0951811.1"
                     /translation="MNLIQQLAGIIEGNLNSPLSVNVLAERSGYSERWLYKLFQDVTG
                     ISVAQYIRRRKLTLAALLLRHTSRRITDISLMYEFSSPQAFSRAFRQQFKVSPVQYRN
                     ADAWDMQYAQPVLYRTTPCTKFRMIEIAEGNHPSVPNLKMPVNLGMDFIHTQNGDHFI
                     YNRNLFESIISIYGPVNEMSDFMISGEMIPSRETDSELIYTFKEINSFSLPETLLPDG
                     LYASQEFTGTYKEIFDFHIYDAMGSLCINKCVMKRGPIYTLFHRTDNKNIMDMQFLIP
                     CVKI"
     gene            2455..3117
                     /locus_tag="ACHKJF_004568"
     CDS             2455..3117
                     /locus_tag="ACHKJF_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase"
                     /protein_id="HFP0951812.1"
                     /translation="MSSRKFLTHHEVSLLLRAVLKGRHPERDACMILLAFIHGLRVSE
                     MLSLRLADLELESRKLCVRRLKNGFSTVHPLLTEEVQCIRNWLKARETIKLPAAEEER
                     DWLFISRSGRPLTRQRFYNLLAEAGRNAGLAVAVHPHMLRHGCGYALADNGIDTRLIQ
                     DYLGHRNIRHTVIYTASNSARFERLWKPSKRAKKQLFDPNCKLPVLMLQMLKKTGYAN
                     NN"
     gene            3484..3948
                     /locus_tag="ACHKJF_004569"
     CDS             3484..3948
                     /locus_tag="ACHKJF_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019076516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiF/GrlA family transcriptional regulator"
                     /protein_id="HFP0951813.1"
                     /translation="MPERRRRIKRHFFCPECVQDMQARPLYLIVTFWGMRTGRLLTVE
                     AVSREFHITQRTAKNILYYIEHEGRNHIRSRRFIQNEPTTGRRRALRILDIQDPQEIP
                     ASSGAELDVQLEPVAPAAKQSLRPLTKFQKLRQWMVSRKQNEQAPLELTEYG"
     gene            3945..4562
                     /locus_tag="ACHKJF_004570"
     CDS             3945..4562
                     /locus_tag="ACHKJF_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0951814.1"
                     /translation="MKQIFLMSQCQYTRLGLFAVMESAGQHAVQVISVETPEQIVSHI
                     LSDGHLPGKQRVIVVDLTCHESSVTVRALWFLWNLSVLYSERHELRGTPCILFGHQEG
                     RNNIRYPFTWISPSQNFHQLKNIFLRILAVPSRYISSGYGFQRLSANERQVINALLGG
                     ETVSDIAMRMQTHYSNVCYYRQKAIFKIGLKNRNDIAWIMNRKFL"
     gene            4884..6104
                     /locus_tag="ACHKJF_004571"
     CDS             4884..6104
                     /locus_tag="ACHKJF_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HFP0951815.1"
                     /translation="MSTLKNTFFIPPPDTPTQAGPENIFYDFNDGARVLLPEGKWHVR
                     LLDADSENILFCCDVDKGWVTSSKKYFVRFRIQVFRQGEETPLLDQTLKLKDRPVLIS
                     FPTGTLGDLLGWFPYAERFQSLHKCRLECTMAQDIIDLLAPQYPQIQFSTPDKPRTVA
                     PYATYRVGLYFGGDTNNQPVDFRKVGFHRSAGYILGVDPREAPVRLDLSAPRVIQEPY
                     VCIATQSTCQAKYWNNGTGWSEVIAHLKSLGYRVLCIDRDAHYGQGFVWNHIPWGAED
                     FTGKLPLQERVNLLRHASFFIGLPSGLSWLAWATGIPVVLISGFSLPNSEFYTPWRIF
                     NSHGCYGCWDDTSLNFDHHDFLWCPRHKNTDRQFECTRLITGAQVNGVINKLHRSLTE
                     QGVEDTLEKGVSNE"
     gene            6097..9819
                     /gene="tibA"
                     /locus_tag="ACHKJF_004572"
                     /gene_synonym="tib"
     CDS             6097..9819
                     /gene="tibA"
                     /locus_tag="ACHKJF_004572"
                     /gene_synonym="tib"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003658957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/invasin glycoprotein
                     TibA"
                     /protein_id="HFP0951816.1"
                     /translation="MNKVYNTVWNESTGMWVVTSELTRKGGQRPRQIKRTVLAGLVAG
                     LLLPSQPVLAAAYDNQTIGSGGTSSSMSLGTGDTATNTTINDGGFQNVNSGGSATSTV
                     INSGGSQLVSSGGSATDTSINKDGLQIVSSGASATNTTIVDFGKQHVSGGSATDTTIN
                     RNGRQYISSGGSATKTTVNSGGRQHVYKNGNVTETIIMDGGRLQVEAGGSASKVTQNS
                     GGAIVTNTGADLSGTNAKGSFSIGGGSAVNMLLENDGYLTVYSGHQASDTTVGSSGTL
                     KVRSGGNAIETTIISGGMLQVEAGGSASKVTQNSGGAVVTNTSADLSGTNAKGNFSIA
                     GGSASNMLLENGGSLTVLNGHQATGTTINSGGDMMVEGIATDTTINSNGLLHVSSGGS
                     VTGTIVNNGGRENINSGGSATSTTLNSGGIQAVSSGGVATSTLVNSGGSQYVYSGGNA
                     LNASVNDGGRQFVSKGGIAIATSINSGGRQYVRIGGSATDTVINNTGRQFISSGGSTT
                     NTTINSGGGQYLYAGNATKTTVNNGGSQYVSSGGSATDTTVNSGGRQNVYIDGNVTET
                     TITSGGMLQVEAGGSASKVTQNSGGAVVTNTSAVVSGTNDNGSFSIAGGSAANMLLEN
                     GGYLTVFDGHQASDTMVGSDGTLDVRSGGVLYGTTTLTDKGTLVGDVVTNEGNLYYLN
                     NSAATFAGTLTGTGTLTQEGGGTRFSGLLSQDGGIFLKSGGAMTMDALQAKANVTTQS
                     GTTLTLDNETILTGNVAGDNTGAGDMAVKGASVWHLDGDSTVGALTLDNGTVDFRPSV
                     TTRLTPAFQAVSLTLGSLSGSGTFRMNTDIASHTGDMLNVAGNASGNFVLDIKNTGLE
                     PVSAGAPLQVVQTGGGDAAFTLKGGKVDAGTWEYGLSKENTNWYLKVDTPPTNPDAGN
                     PGTGNPDAGKPDAGTSSSPVRRTTKSADAVLGMATAPAYVFNSELDNLRFRHGDVMQN
                     TRAPGGVWGRYTGSDNRISGGASSGYTLTQNGFETGADMVFDLSDSSLAVGTFFSYSD
                     NSIKHARGGKSNVDSTGGGLYATWFDNDGYYVDGVLKYNRFNNELRTWMSDGTAVKGD
                     YSQNGFGGSLEAGRTFSLNENAWAQPYVRTTAFRADEKDIRLNNGMKASIGATKSLQA
                     EAGVKLGMTLDVTGKEVKPYLSAAVSHEFSDNNKVRINDTYDFRNDISGTTGKYGLGV
                     NAQLTPNAGVWAEARYENGKQTESPITGSVGFRINF"
     gene            9914..>10225
                     /locus_tag="ACHKJF_004573"
     CDS             9914..>10225
                     /locus_tag="ACHKJF_004573"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0951817.1"
                     /translation="MENKKNIRYIKTNIIEHDVIVHIWIYTPLTKVECDVFELLVKGY
                     KIANVAQYRARSLKTVSSQKHQVYKKLGIRNDVTFWIDIILSHHMRIVFCRNGKVIDT
                     EK"
BASE COUNT         2709 a         2339 c         2605 g         2572 t
ORIGIN      
        1 ttcagtatat ttggcaatgg cggacttttg tcttcgttta ctcggtagtg atgtttaacc
       61 ggctgtcgac ctgtcagccc gcattcccgc atcagccgtc gtgccagcca ccggcctgca
      121 tcaacgcaac tctggcgcaa catctgactg aatgctaggc taccggctgc gccacgactg
      181 agagcatgga aagccctcac ccgacttcgt aattcaattt tttgcacgct tcacctgcgt
      241 gtaataaacg ctacggtgaa taccgaataa atgacaaaga acccgcactg gccactttac
      301 tttcagccgt gtgattagcg cgacagctta ccggggattt tgcttatcaa cacggcagcc
      361 tactttagtg tttctttttc catctcaggg cgctttatct gcgctttaag ctgctgaatt
      421 tcgcgttgtt caggggtaat agcatttcaa taccctgaag ttcctgctta tacaaccgta
      481 tccatttacg caaatggtca gggttgagct cgagtgccag cgcgacttat ctgacatcac
      541 gctggtattt aaccgcctgc tcgatagctt caagcttgaa ctccggggaa aaggtacgtt
      601 tagtccgacg agttttgatc atgcatcacc tcattttcac tgttttaaca ttaacaggat
      661 ttcgaggtgt cctgaattac cgatccacta cacaccaacg aacgaccatc gtgtgaagaa
      721 gttattgccc tggatgtaaa ctgatttttc caccacgtct cagtataggg gcttacttat
      781 tgtctttgaa gaccaagcaa gataacgtca atacggcgtt cgggtaacgc ttacaacacc
      841 cacttccgaa actgataagt cttcctttta tctaaaaatg taaaatttac atataactct
      901 gacaggagga aatatgcgta aatacatcac caacagcgaa tgggcgctct tttttaatgc
      961 tataacgggt acgcgcaatg aaatccgtga taaagcattg ctgcaaataa cttacattca
     1021 tggacttcgc gtcagtgaat tgatagacct tagactggga gattttgata tgactgaaaa
     1081 aattgttttt attaggcggc tgaaaaacgg attatcaact tcacatccat tgcagccaga
     1141 aacaatagaa ctattgcaac gctggttcct gatcagaaaa agatacacta aaggtagtca
     1201 ggatgattat ctttttctgt caagccatgg gaaaaaaatt tccagacaat ggatttataa
     1261 actctgcgca cgatatagtc aacgtgcggg tctgagaatt aaggtgcatc cacacatgtt
     1321 gcgtcacgcc tgtggttatg ctcttgctaa tcagggactg gatactcgtc tgattcagga
     1381 ttatctggga cacaggaaca ttcatcatac tgtactgtat acagccagta atcctggtcg
     1441 ttttaatcgg gtctggcgaa gttaaatttt tacgcaagga atgaggaatt gcatatccat
     1501 aatattttta ttatctgttc tgtgaaatag tgtatatata ggtcctcttt tcataacaca
     1561 tttattaata cacagcgacc ccatggcatc atatatatga aaatcaaaaa tctctttgta
     1621 tgtcccagta aattcctggc tggcatataa accatcagga agaagcgtct ctggcaggga
     1681 aaaactgttt atttctttaa aagtataaat tagttcagaa tccgtttctc tggagggtat
     1741 catttcgcct gaaatcataa agtcagacat ttcattaaca ggaccataaa ttgatattat
     1801 ggactcaaaa aggttacggt tataaataaa atgatcgccg ttctgggtat ggataaagtc
     1861 cattcccaga ttcacaggca ttttaaggtt tggaacagaa ggatgattgc cctcagctat
     1921 ctcaatcatt ctgaattttg tgcacggtgt ggttctgtac aatacgggct gggcatactg
     1981 catatcccag gcatccgcat tgcgatactg cactggtgac accttgaact gttgcctgaa
     2041 agcacgggaa aaagcctggg gcgaactgaa ctcatacatt agtgatatgt ctgttatccg
     2101 gcgactggta tggcgcaaaa gtaaagcggc aagtgttagt tttctgcgac gtatgtactg
     2161 cgctacactg atccctgtta catcctgaaa cagtttgtac aaccagcgtt ctgaatagcc
     2221 tgaacgttca gcaagaacgt ttactgacag aggactgttc agattccctt cgataattcc
     2281 cgccagttgc tggattaaat tcatttgata tacctgtggg tccatgccag ttgactgttc
     2341 ttaagagcaa tcggagtttt attctgcttt cctgacttta gcacgtttag ccatagcctg
     2401 atgggggcat cgtgtcactc ttgaccgctc tgtttcatct gaccggagct attaatgtct
     2461 tcccgaaaat ttcttactca ccatgaagtc agccttttgt tacgggcggt gctgaagggg
     2521 cgccacccgg aacgtgatgc ctgcatgatt cttcttgctt ttatccacgg actcagggtc
     2581 agcgaaatgc tttcgttacg tcttgctgac cttgagctcg agtcccgaaa gctgtgcgtt
     2641 cggcgcctga aaaacggctt cagtaccgtg catcccttac tgactgaaga agtacaatgt
     2701 atcaggaact ggctcaaagc ccgggaaact ataaaattgc ctgcagcaga agaagagagg
     2761 gattggctat ttatttccag atcagggcgt ccgcttaccc gtcaacgttt ttataacttg
     2821 ctggctgaag caggaagaaa tgccgggctg gctgttgctg tccatccaca tatgctgaga
     2881 catgggtgtg gttacgcgtt ggcagataac ggcatcgata cacgcctgat tcaggattac
     2941 ctgggacacc ggaatatccg acatacggtg atttatacag cttcaaattc ggcccgcttt
     3001 gaacggttat ggaagccgtc aaaaagggcg aaaaagcaac tttttgaccc aaactgtaaa
     3061 ctgcctgtat tgatgttaca aatgttaaaa aaaacaggtt atgcaaacaa caattaatta
     3121 aaaatcgttt gatctaaatc aataaaactc ccgtatcaaa tcccttctgt aacaatatgg
     3181 cattgatgtc tgtcagtttt ctgaattttc caaaactcca ttaacagtat gaatggccaa
     3241 aaacaccaca cacctaagaa aataaaacca cacaaaagcc aaaatctagt tttttaatct
     3301 attgttgtca aaatgggtca ttttgacaac aacagttcct cttcttgtgc acctgtattg
     3361 cgcattactc tgcactgata acccgacaca tccggcaaca gatgtttgtt gtaattactg
     3421 gggggaggga tgtcagtgct tcttaattca tacttctgac ggagagatat tcaggtggcg
     3481 ggtatgcctg agagacgcag acgaataaag agacattttt tctgtcctga gtgtgttcag
     3541 gacatgcagg ctcgaccact gtatctcatc gtgaccttct gggggatgcg aaccggcagg
     3601 ttactgacag ttgaggcggt cagccgggag tttcatataa cccagagaac agcaaaaaat
     3661 attctgtatt acattgaaca cgagggacga aaccatatcc gcagcaggcg ctttatacaa
     3721 aacgaaccga caactggacg aagacgcgca ctgcggatcc tggatataca ggatccgcag
     3781 gaaatcccgg ccagttcagg agctgaactg gatgttcagt tggaacctgt tgcccccgca
     3841 gcaaagcagt cactcaggcc actgacaaaa tttcagaaac tacgtcagtg gatggtatcg
     3901 agaaaacaga atgaacaggc tcctctggag ctgacagaat acggatgaaa cagatatttt
     3961 tgatgagtca gtgtcagtac acccgcctcg ggctttttgc agtgatggaa agtgccgggc
     4021 aacatgctgt tcaggttatt tccgtggaaa caccggagca gattgtgtcg catattctgt
     4081 ctgacggaca cctcccgggg aaacagcgtg tcattgtggt agatctgacc tgccatgagt
     4141 catcagtgac tgtgagggcg ctgtggtttt tgtggaatct gtcagtcctt tattctgaac
     4201 gccacgaact gagagggact ccatgcattt tatttggaca tcaggaaggg cggaataata
     4261 tcagatatcc ttttacctgg atttctccgt cacaaaactt tcatcaattg aaaaatattt
     4321 ttctgcgcat tctggccgtc ccttctcgct atataagtag tgggtatgga tttcagcgtt
     4381 taagtgctaa tgagcgacag gtgataaatg cacttcttgg cggggaaact gtaagtgata
     4441 ttgcaatgcg tatgcagaca cattacagca atgtgtgtta ctaccgacaa aaggcaatat
     4501 ttaaaatcgg tctgaaaaac cggaatgata ttgcatggat tatgaacagg aagtttttgt
     4561 aagcactgta acggtacgta ctgaccggat acacttccga aattgataag tctttccgca
     4621 cgtaaactgt aaactaactc ctgcgcgtag ccggaagtta tgcagagagc atttcatcgg
     4681 caacgactaa agtcgttata actataatgg aatgcgcgat gtgtgacatc gtaagaatgt
     4741 aatatgtgag ctcctctctg ctattacaga agaatatcag aaccgtgcga tgggtgttac
     4801 tcatcataag aaagatactt cagttatatt tttagcgtac tcatcgtgct tttcaacgct
     4861 tatatatttt tgaaggtgaa aatatgtcaa cgctgaagaa tacatttttt atccctccac
     4921 ctgatacgcc gacgcaggca gggcctgaaa atatttttta tgactttaat gatggtgcac
     4981 gtgtattgct tcctgaaggg aaatggcatg tgcgtctgct ggatgcggat tcagaaaata
     5041 ttcttttctg ctgcgatgtg gataaaggct gggtaaccag cagtaagaaa tattttgtgc
     5101 gctttcgtat tcaggtattc cgtcaggggg aagaaacgcc cctgctggat caaaccctga
     5161 aattaaaaga ccggcctgta ctaatttcat tcccgactgg gacgctgggc gatttgctcg
     5221 gctggtttcc ttatgcggaa cgcttccagt ccctgcataa gtgccgtctg gaatgcacga
     5281 tggcgcagga cattattgat ctgctggcgc ctcagtatcc gcagatacag ttttccacgc
     5341 cggataagcc gcgcactgta gcgccttatg ccacctatcg tgtggggctc tatttcggtg
     5401 gcgatacaaa caatcagcct gtggatttcc gcaaggtcgg tttccaccgc tctgcgggct
     5461 acattctggg cgttgacccg cgtgaagccc ccgtccggct tgacctgtct gccccccggg
     5521 ttatccagga gccttatgtg tgcatcgcca cgcagtccac ctgccaggcg aaatactgga
     5581 acaacggcac cggctggagt gaggttatcg cacacctgaa atcactgggt taccgggtgc
     5641 tgtgcattga ccgggatgcc cactatggcc agggctttgt ctggaatcac atcccgtggg
     5701 gtgcagagga ctttaccggc aagcttccgc tgcaggagag ggtgaaccta ctgcggcatg
     5761 ccagtttctt cattggcctg ccaagcggct tgtcatggct ggcctgggca accggtattc
     5821 cggtggtgct gataagcggc ttcagcctgc ctaacagcga gttttatacc ccgtggcgga
     5881 tattcaactc ccatggctgt tatgggtgct gggacgatac atcgctgaat tttgaccacc
     5941 acgattttct gtggtgcccg agacataaaa acactgaccg gcaatttgaa tgtactcggc
     6001 tgataactgg cgctcaggtc aatggcgtca tcaataaact tcaccgttct ttaactgaac
     6061 agggtgttga ggacactctg gagaaaggag taagcaatga ataaggttta taacactgtc
     6121 tggaatgaat ccaccgggat gtgggtcgtt acctcagaac tgacccgtaa aggaggtcag
     6181 cgcccccggc aaatcaagcg taccgtgctg gcagggctgg ttgctggttt gcttttgccg
     6241 tcacagcctg tactggcggc agcctatgac aatcagacaa ttggaagtgg tggaacaagc
     6301 tcatccatgt ccctgggtac gggtgatacg gcgacaaata ccaccattaa cgatggaggg
     6361 tttcagaatg tcaacagcgg cggtagtgcc acgagtaccg tcattaatag tggtgggagc
     6421 caactagtat ccagtggcgg tagtgctacg gacaccagta ttaataaaga tgggcttcag
     6481 attgtatcca gcggagcaag tgccacaaat acaaccattg ttgacttcgg gaagcagcat
     6541 gtcagcggcg gaagtgccac ggacaccact attaacagga atggacgcca atatatctcc
     6601 agtggtggca gtgccactaa aacaaccgtt aacagtggcg ggcgtcagca tgtttataaa
     6661 aacggcaatg tgacagaaac gatcattatg gacggcggca ggctgcaggt ggaagccggt
     6721 ggctcagcct ctaaggttac tcagaacagt ggcggtgcta tcgttactaa taccggtgca
     6781 gacctgagtg gtaccaacgc taaaggcagc ttcagtatag gtggcggcag tgcagtcaat
     6841 atgctgctgg agaacgatgg ctacctgacg gtttacagtg gtcatcaggc cagcgataca
     6901 accgttggca gcagcgggac gctgaaagtc cgcagcggcg gaaatgccat agaaacgacg
     6961 attatcagcg gcggcatgct gcaggtggaa gccggtggct cagcctctaa ggttactcag
     7021 aacagtggcg gtgctgtcgt tactaatacc agtgcagacc tgagtggtac caacgctaaa
     7081 ggcaatttca gcatcgctgg cggcagtgct tccaatatgc ttctggagaa tggtggctca
     7141 ttaactgtac tcaatgggca tcaggccaca ggtaccacta ttaacagtgg tggagatatg
     7201 atggtcgagg gcatcgctac tgacacaacc attaacagca atggcttact gcatgtatcc
     7261 agcggcggta gtgtcactgg caccatcgtt aataacggtg gccgcgagaa tatcaattca
     7321 ggcggcagtg ccacgagcac tacccttaac agtggtggta tccaggctgt ctccagcggc
     7381 ggtgttgcta cgagcaccct cgttaacagc ggagggagcc aatatgtcta cagcggcggc
     7441 aatgcgttga acgcttctgt taatgacggt gggcgtcagt ttgtctccaa aggcggtatc
     7501 gccattgcta cttctattaa tagcggcggt cgccagtatg tcagaatcgg cggcagtgcc
     7561 acggacaccg ttattaacaa tactgggcgc cagtttatct ccagcggcgg cagtaccact
     7621 aacactacaa ttaattccgg tggggggcag tatctctacg ccggaaatgc cacgaaaact
     7681 actgttaaca acggcgggag ccagtacgtc tccagtggag gcagcgccac tgacacaacc
     7741 gttaacagtg gtggacgtca gaatgtttac atcgacggaa atgtcacaga aacgactatt
     7801 acaagcggcg gcatgctaca ggtggaggcc ggtggttcag cctctaaagt tacccagaac
     7861 agtggtgggg ctgtcgtcac caataccagt gcagtcgtga gtggtaccaa cgataacggc
     7921 agcttcagta tcgctggcgg cagtgcagcc aatatgctgc tggaaaatgg tggatacctg
     7981 acggtgtttg acggtcatca ggccagcgat acgatggttg gcagtgacgg tacgctggat
     8041 gttcgcagcg gcggtgtgct ctacggcacc acaaccctga ctgataaagg gacgttggtc
     8101 ggtgacgtag tcacaaacga aggcaacctg tattacctca acaacagtgc tgcgaccttc
     8161 gcaggcaccc tgacggggac cggcaccctg acgcaggaag gcggtggcac ccgcttcagc
     8221 ggcctgttgt ctcaggacgg tgggattttc cttaagagtg gcggtgccat gacgatggat
     8281 gcacttcagg ctaaggctaa tgtgacgaca cagtcaggca ccaccctgac gctggataac
     8341 gagaccatcc tgacggggaa cgttgcggga gacaacaccg gtgcaggtga tatggcggtt
     8401 aaaggtgcgt ctgtctggca tctcgacggc gactccactg tcggggcatt aacgctggat
     8461 aacgggactg ttgatttccg tccgtcagta accactcgtc tgacgccagc cttccaggcg
     8521 gtgtcgctga cgctcgggag cctgtcgggt agcgggacgt tccggatgaa cacggatatc
     8581 gcatcacata ccggagatat gctgaatgtc gcgggcaatg ccagcggtaa ctttgtgctg
     8641 gatatcaaaa acaccggtct ggagccagta tctgctggtg cgccgcttca ggtggtgcag
     8701 acaggtggcg gtgatgccgc atttaccctg aaaggcggga aagtcgatgc cggtacctgg
     8761 gaatacggcc tgagcaagga aaataccaac tggtatctga aggttgatac tccgccgact
     8821 aacccggatg caggtaaccc aggtacaggt aaccctgacg caggtaagcc agatgcaggt
     8881 acatcgtcgt ctccggtgcg tcgcacaaca aaatcggccg acgcagtact gggcatggcg
     8941 acagcaccgg catacgtctt caacagcgag ctggataatc tgcgtttccg tcatggtgat
     9001 gtgatgcaga atactcgtgc accagggggc gtatggggcc gttataccgg gtcagacaac
     9061 cgcattagcg gcggggccag ctcgggctat accctgaccc agaacggttt tgaaaccggc
     9121 gccgatatgg tctttgacct gagtgacagc agtttagcgg taggtacttt cttctcttac
     9181 agcgacaaca gcattaaaca tgcccgtgga ggaaagagta acgttgacag cactggtggt
     9241 ggtctgtacg cgacctggtt tgataatgac ggctactacg tggacggtgt gctgaaatac
     9301 aaccgcttta ataatgagct gcgtacctgg atgagtgacg gcacggcggt gaagggcgat
     9361 tacagccaga acggtttcgg cggtagcctc gaagcgggca gaaccttcag tctgaatgag
     9421 aatgcatggg cgcagccata cgtgcggacg acggcattcc gggcagatga gaaggatatc
     9481 cgactgaata acggcatgaa agccagtatc ggtgcgacca aatccttgca ggctgaggct
     9541 ggcgtgaagc tggggatgac cttggacgtt acaggaaaag aggtcaaacc gtacctgtcg
     9601 gcggcggtga gtcatgaatt ttcggacaac aacaaagtcc gtatcaatga cacctatgac
     9661 ttcaggaacg atatctccgg gacgaccggg aaatacgggc tgggtgtcaa tgctcaactg
     9721 accccaaatg ctggcgtctg ggctgaagct cgttatgaaa acggtaagca gaccgagtca
     9781 ccaataactg gtagcgttgg tttccgaatc aacttctgat gaatgggcag ggatacgtat
     9841 tagattttcg atgtgaatcc ctgcctgctt tcagtaaaga ttaatgaaat ctaattttgt
     9901 taggagtgtt ataatggaaa ataaaaaaaa catccgttac ataaaaacta atataataga
     9961 gcatgatgtt atagttcata tatggatata tacgcctctg acaaaagttg aatgcgatgt
    10021 gttcgagttg ctagtaaaag gatataagat cgctaatgta gctcaataca gagctcgtag
    10081 cctcaaaaca gtttcatcac aaaaacatca agtatataaa aaacttggga tccgtaatga
    10141 tgttacgttc tggatagata taattttatc acatcacatg agaatcgttt tttgtcgaaa
    10201 tgggaaagtt attgatacag aaaaa
//
LOCUS       DAZGXZ010000168        10191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000168 DAZGXZ010000000
VERSION     DAZGXZ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.168"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(77..853)
                     /gene="yaaA"
                     /locus_tag="ACHKJF_004574"
     CDS             complement(77..853)
                     /gene="yaaA"
                     /locus_tag="ACHKJF_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP0951818.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(923..2353)
                     /locus_tag="ACHKJF_004575"
     CDS             complement(923..2353)
                     /locus_tag="ACHKJF_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP0951819.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            2632..3585
                     /gene="tal"
                     /locus_tag="ACHKJF_004576"
     CDS             2632..3585
                     /gene="tal"
                     /locus_tag="ACHKJF_004576"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0951820.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            3700..4287
                     /gene="mog"
                     /locus_tag="ACHKJF_004577"
     CDS             3700..4287
                     /gene="mog"
                     /locus_tag="ACHKJF_004577"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP0951821.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(4322..4888)
                     /gene="satP"
                     /locus_tag="ACHKJF_004578"
     CDS             complement(4322..4888)
                     /gene="satP"
                     /locus_tag="ACHKJF_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP0951822.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5037..5750)
                     /gene="msyB"
                     /locus_tag="ACHKJF_004579"
     CDS             complement(5037..5750)
                     /gene="msyB"
                     /locus_tag="ACHKJF_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0951823.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(5776..6180)
                     /locus_tag="ACHKJF_004580"
     CDS             complement(5776..6180)
                     /locus_tag="ACHKJF_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP0951824.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            6557..8473
                     /gene="dnaK"
                     /locus_tag="ACHKJF_004581"
     CDS             6557..8473
                     /gene="dnaK"
                     /locus_tag="ACHKJF_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP0951825.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8562..9692
                     /gene="dnaJ"
                     /locus_tag="ACHKJF_004582"
     CDS             8562..9692
                     /gene="dnaJ"
                     /locus_tag="ACHKJF_004582"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP0951826.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(9796..9948)
                     /gene="mokC"
                     /locus_tag="ACHKJF_004583"
     CDS             complement(9796..9948)
                     /gene="mokC"
                     /locus_tag="ACHKJF_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP0951827.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2667 a         2556 c         2636 g         2332 t
ORIGIN      
        1 tctgcaatat attgaatctg catgcttttg taggccggat aaggcgttca cgccgcatcc
       61 ggcattgact acaaacttaa cgctgctcgt agcgtttaaa caccagttcg ccattgctgg
      121 aggaagcttc atcaaagaag taaccttcgc tattaaaacc agtcagttgc tctggtttgg
      181 tcagccgatt ttcaataata aaacgactca tcagaccgcg tgctttctta gcgtagaagc
      241 tgattatctt aaatttgccg ttcttctcat cgaggaacac cggcttgata atctcggcat
      301 tcaatttctt cggcttcacc gatttaaaat actcatctga cgccagatta atcaccacat
      361 tatcgccttg tgctgcgagc gcctcgttca gcttgttggt gatgatatct ccccagaatt
      421 gatacagatc tttccctcgg gcattctcaa gacggatccc catttccaga cgataaggct
      481 gcattaaatc gagcgggcgc agtacgccat acaagccgga aagcattcgc aaatgctgtt
      541 gggcaaaatc gaaatcgtct tcgctgaagg tttcggcctg caagccggtg tagacatcac
      601 ctttaaacgc cagaatcgcc tggcgggcat tctccggcgt gaaatctggc tgccagtcat
      661 gaaagcgagc ggcgttgata cccgccagtt tgtcgctgat gcgcatcagc gtgctaatct
      721 gcggaggcgt cagtttccgc gcttcatgga tcaactgctg ggaattgtct aacagctccg
      781 gcagcgtata gcgcgtggtg gtcaacgggc tttggtaatc aagcgttttc gcaggtgaaa
      841 taagaatcag catatccagt ccttgcagga aatttatgcc gactttagca aaaaaagaga
      901 atgagttgat cgatagttgt gattactcct gcgaaacatc atcccacgcg tccggagaaa
      961 gctggcgacc gatatccgga taacgcaacg gatcaaacac cgggcgcacg ccgagtttac
     1021 gctggcgtag ataatcactg gcaatggtat gaaccacagg cgagagcagt aaaatggcgg
     1081 tcaaattggt aatagccatg caggccatta tgatatctgc cagttgccac atcagcggaa
     1141 ggcttagcaa ggtgccgccg atgaccgttg cgaaggtgca gatccgcaaa caccagatcg
     1201 ctttagggtt gttcaggcgt aaaaagaaga gattgttttc ggcataaatg tagttggcaa
     1261 cgatggagct gaaggcaaac agaataacca cgagggtaac aaactcagca ccccaggaac
     1321 ccattagcac ccgcatcgcc ttctggataa gctgaatacc ttccagcggc atgtaggttg
     1381 tgtcgttacc cgccagtaat atcagcatgg cgcttgccgt acagatgacc agggtgtcga
     1441 taaaaatgcc aatcatctgg acaatccctt gcgctgccgg atgcggaggc caggacgccg
     1501 ctgccgctgc cgcgtttggc gtcgaaccca ttcccgcctc attggaaaac atactgcgct
     1561 gaaaaccgtt agtaatcgcc tggcttaagg tatatcccgc cgcgccgcct gccgcttcct
     1621 gccagccaaa agcactctca aaaatagacc aaatgacgtg gggaagttgc ccgatattta
     1681 ttacgcaaat caccatgctg gtcagcaccc agattatcgc catcaacggg acaaacccct
     1741 gcatgagccg ggcgacgcca tgaagaccgc gagtgattgc cagcagagca aagacagcga
     1801 gaataatgcc tgtcaccagc ggaggaaaat caaaagaaaa acttagggcg cgggcaacgg
     1861 cgttcgcctg aactccgctg aaaattatgc cataggcgat gagcaaaaag acggcgaaca
     1921 gaacgcccat ccaacgcatc cccagcccgc gcgccatata ccatgccggt ccgccacgaa
     1981 actgtccatt gacgtcacgt tctttataaa gttgtgccag agaacattcg gcaaacgagg
     2041 tcgccatgcc gataaacgcg gcaacccaca tccaaaagac ggctccaggt ccaccggcgg
     2101 taattgccag cgcaacgccg gccaggttgc cgctacccac gcgcgccgca agactggtac
     2161 acaatgactg aaatgaggtt aaaccgcctg gctgtggatg aatgctattt ttaagacttt
     2221 tgccaaactg gcggatgtag cgaaactgca caaatccggt gcgaaaagtg aaccaacaac
     2281 ctgcgccgaa gagcaggtaa atcattaccg atccccaaag gacgctgtta ataaaggaga
     2341 aaaaatctgg catgcatatc cctcttattg ccggtcgcga tgactttcct gtgtaaacgt
     2401 taccaattgt ttaagaagta tatacgctac gaggtacttg ataacttctg cgtagcatac
     2461 atgaggtttt gtataaaaat ggcgggcgat atcaacgcag tgtcagaaat ccgaaacagt
     2521 ctcgcctggc gataaccgtc ttgtcggcgg ttgcgctgac gttgcgtcgt gatatcatca
     2581 gggcagaccg gttacatccc cctaacaagc tgtttaaaga gaaatactat catgacggac
     2641 aaattgacct cccttcgtca gtacaccacc gtagtggccg acactgggga catcgcggca
     2701 atgaagctgt atcaaccgca ggatgccaca accaaccctt ctctcattct taacgcagcg
     2761 cagattccgg aataccgtaa gttgattgat gatgctgtcg cctgggcgaa acagcagagc
     2821 aacgatcgcg cgcagcagat cgtggacgcg accgacaaac tggcagtaaa tattggtctg
     2881 gaaatcctga aactggttcc gggtcgtatc tcaactgaag ttgatgcgcg tctttcctat
     2941 gacaccgaag cgtcaattgc gaaagcaaaa cgcctgatca aactctacaa cgatgctggt
     3001 attagcaacg atcgtattct gatcaaactg gcttctacct ggcagggtat ccgtgctgca
     3061 gaacagctgg aaaaagaagg catcaactgt aacctgaccc tgctgttctc cttcgctcag
     3121 gctcgtgctt gtgcggaagc aggcgtgttc ctgatctcgc catttgttgg ccgtattctt
     3181 gactggtaca aagcgaatac tgataagaaa gagtacgctc cggcagaaga tccgggcgtg
     3241 gtttctgtat ctgaaatcta ccagtactac aaagagcacg gttatgaaac cgtggttatg
     3301 ggcgcaagct tccgtaacat cggcgaaatt ctggaactgg caggctgcga ccgtcttacc
     3361 atcgcaccta cactgctgaa agagctggct gagagcgaag gggctatcga acgtaaactg
     3421 tcttacaccg gcgaagtgaa agcgcgtccg gcgcgtatca ctgagtccga gttcctgtgg
     3481 cagcacaacc aggatccaat ggcagtagat aaactggcgg aaggtatccg taagtttgct
     3541 attgaccagg aaaaactgga aaaaatgatc ggcgatctgc tgtaatcatt cttagcgtga
     3601 ccgggaagtc ggtcacgcta cctcttctga agcctgtctg tcactccctt cgcagtgtat
     3661 cattctgttt aacgagactg tttaaacgga aaaatcttga tgaatacttt acgtattggc
     3721 ttagtttcca tctctgatcg cgcatccagc ggcgtttatc aggataaagg catccctgcg
     3781 ctggaagaat ggctgacatc ggcgctaacc acgccgtttg aactggaaac ccgcttaatc
     3841 cccgatgagc aggcgatcat cgagcaaacg ttgtgtgagc tagtggatga aatgagttgc
     3901 catctggtgc tcaccacggg cggaactggc ccggcgcgtc gtgacgtaac gcccgatgcg
     3961 acgctggcag tagcggaccg cgagatgcct ggctttggtg aacagatgcg ccagataagc
     4021 ctgcattttg taccaactgc gatcctttcg cgtcaggttg gtgtgattcg caaacaggcg
     4081 ctgatcctta acttacccgg tcagccgaag tctattaaag agacgctgga aggtgtgaag
     4141 gacgctgcgg gtaacgttgt ggtacacggt atttttgcca gcgtaccgta ctgcattcag
     4201 ttgctggaag ggccatacgt tgaaacggca ccggaagtgg ttgcagcatt cagaccgaag
     4261 agtgcaagac gcgacgttag cgaataaaaa aatccccccg agcgggggga tctcaaaaca
     4321 attagtggga ttcaccaatc ggcagaacgg tgcgaccaaa ctgctcgttc agtacttcac
     4381 ccatcgccag atagattgcg ctggcaccgc agatcagccc aatccagccg gcaaagtgga
     4441 tgattgcggc gttaccggca atgttaccga tcgccagcag ggcaaacagc acggtcaggc
     4501 taaagaaaac gaattgcaga acgcgtgcgc ctttcagcgt gccgaagaac ataaacagcg
     4561 taaatacgcc ccacagaccc aggtagacac caaggaactg tgcatttggc gcatcggtca
     4621 gacccagttt cggcatcagc agaatcgcaa ccagcgtcag ccagaaagaa ccgtaagagg
     4681 tgaatgcggt taaaccgaaa gtgttgcctt ttttgtactc cagcagacca gcaaaaattt
     4741 gcgcgatgcc gccgtagaaa atgcccatgg caagaataat accgtccaga gcgaaataac
     4801 ccacgttgtg caggttaagc agaatggtgg tcatgccgaa gcccatcagg cccagcggtg
     4861 ccggattagc caacttagtg ttgcccataa ttcctcaaaa atcatcatcg aatgaatggt
     4921 gaaataattt ccctgaataa ctgtagtgtt ttcagggcgc ggcataataa tcagccagtg
     4981 gggcagtgtc tacgatcttt tgaggggaaa atgaaaattt tccccggttt acggtatcag
     5041 acctgagtgg cgctaaccat ccggcgcagg caggcgattt gcagtacggc tggaatcgtc
     5101 acgcgatagg cgctgccgct gaccgcttta accccattta gtgccgcacc tacagggcct
     5161 cccagccccg cgccgcgcag caaaccatgc ccaagtacgc tcattgctgc gtgggtgcgt
     5221 aaaatgcggg tcagttggct ggaaagcaaa tgcgacacgc cttttgccaa taatttgtct
     5281 ttcatcagca gcggcagcag ctcttccagc tcattcaccc tcgcatcgac cgcgtgcaga
     5341 aactcctgtt tatgttcctc gtccatattc ttccaggtgt tacgcagaaa ttgttccagt
     5401 aactgttgct caatctcaaa cgtagacatc tctttgtcgg ctttcagctt caatcgtttt
     5461 gaaacatcga gcaaaatggc ccgatacaat ttaccgtgtc cgcgcagttt gttggcgata
     5521 ctatcgccac caaaatgctg taattctcca gcaatcagct gccagttgcg gcgatgttgc
     5581 tcgggatgcc cttccatcga tttaaacagt tcgttgcgca tcagtacgct ggagaggcga
     5641 gttttgcctt tttcattatg ggtgagcaat cgggcgaaat ttgccaactg ttcctcactg
     5701 caatgctgga gaaaatccag atctgaatca ttcaggtaat taacattcat tttttgtggc
     5761 ttctatattc tggcgttagt cgtcgccgat aattttcagc gtggccatat ccgatgagtt
     5821 caccgtatga ccggaaaagg tgatttttga gacgcagcgt ttattgtcgt tatcgctgtt
     5881 aatgttgatc cagtcagtgg tttgcccttc ttttatttct gaaggaatat tcaggctctg
     5941 actggcgcta cgggcggctt tgaaataaac cgatgcaccg cttaactgta aatcgccatg
     6001 gtcggcagag agttgtatgc gtttaacaat tcggcaaaca ggaagtttca gcgccagatc
     6061 gttggtttcg ttacgtggca tcgcaatgac gccgaggatt ttatggtcgt ttgcctgcgc
     6121 cgtgcagcac agcatcagac taatcgccag gctggcggaa atcgtaaaaa cggatttcat
     6181 aaggattctc ttagtgggga gaggtagggg gatggatacc cactagttta ctgctgataa
     6241 agagaagatt caggtgcgta atgttttctt tttattacaa ttttttgatg aatgtcttgg
     6301 ctgcgattca ttctttatat gaataaaatt gctgtcagtt tcacgtcttg tcctgctata
     6361 tcgcgaaatt tctgcgcaaa agcacaaaaa atttttgcat ctcccccttg atgacgtggg
     6421 ttacgacccc atttagtagt caaccgcagt gagtgagtct gcaaaaaaat gaaattgggc
     6481 agttgaaacc agacgtttcg cccctattac agactcacaa ccacatgatg accgaatata
     6541 tagtggagac gtttagatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg
     6601 tgtagcgatt atggatggca ccactcctcg cgtgctggag aacgccgaag gcgatcgcac
     6661 cacgccttct atcattgcct atacccagga tggtgaaact ctggttggtc agccggctaa
     6721 acgtcaggca gtgacgaacc cgcaaaacac cctgtttgcg attaaacgcc tgattggccg
     6781 ccgcttccag gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc
     6841 tgctgataac ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat
     6901 ctctgctgaa gtgctgaaaa aaatgaagaa aaccgctgaa gattacttgg gtgaaccggt
     6961 aactgaagct gttatcaccg taccggcata ctttaacgat gctcagcgtc aggcaaccaa
     7021 agacgcaggc cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac cgaccgcagc
     7081 tgcgctggct tacggtctgg acaaaggtac tggcaaccgt actatcgcgg tttatgacct
     7141 gggtggtggt actttcgata tttctattat cgaaatcgac gaagttgacg gcgaaaaaac
     7201 cttcgaagtt ctggctacca acggtgatac ccacctgggt ggtgaagact tcgacagccg
     7261 tctgatcaac tacctggttg aagaattcaa gaaagatcag ggcattgacc tgcgcaacga
     7321 tccgctggca atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg aactgtcttc
     7381 cgctcagcag accgacgtta acctgccgta catcactgca gatgcgaccg gtccgaaaca
     7441 catgaacatc aaagtgactc gtgcgaaact ggaaagcctg gttgaagatc tggtaaaccg
     7501 ttccattgag ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat ctgatatcga
     7561 cgacgttatc ctcgttggtg gtcagactcg tatgccaatg gttcagaaga aagttgctga
     7621 gttctttggt aaagagccgc gtaaagacgt taacccggac gaagctgtag caatcggtgc
     7681 tgctgttcag ggtggtgttc tgactggtga cgtgaaagac gtactgctgc tggacgttac
     7741 cccgctgtct ctgggtatcg aaaccatggg cggtgtgatg acgacgctga tcgcgaaaaa
     7801 caccactatc ccgaccaagc acagccaggt gttctctacc gctgaagaca accagtctgc
     7861 ggtaaccatc catgtgctgc agggtgaacg taaacgtgcg gctgataaca aatctctggg
     7921 tcagttcaac ctggatggta tcaacccggc accgcgcggc atgccgcaga tcgaagttac
     7981 cttcgatatc gatgctgacg gtatcctgca cgtttccgcg aaagataaaa acagcggtaa
     8041 agagcagaag atcaccatca aggcttcttc tggtctgaac gaagatgaaa tccagaaaat
     8101 ggtacgcgac gcagaagcta acgccgaagc tgaccgtaag tttgaagagc tggtacagac
     8161 tcgcaaccag ggcgaccatc tgctgcacag cacccgtaag caggttgaag aagcaggcga
     8221 caaactgccg gctgacgaca aaactgctat cgagtctgca ctgactgcac tggaaactgc
     8281 tctgaaaggt gaagacaaag ccgctatcga agcgaaaatg caggagctgg cacaggtttc
     8341 ccagaaactg atggaaatcg cccagcagca acatgcccag cagcagactg ccggtgctga
     8401 tgcttctgca aacaacgcga aagatgacga tgttgtcgac gctgaatttg aagaagtcaa
     8461 agacaaaaaa taatcgccct ataaacgggt aattatactg acacgggcga aggggaattt
     8521 cctctccgcc cgtgcattca tctaggggca atttaaaaaa gatggctaag caagattatt
     8581 acgagatttt aggcgtttcc aaaacagcgg aagagcgtga aatcaaaaag gcctacaaac
     8641 gcctggccat gaaataccac ccggaccgta accagggtga caaagaggcc gaggcgaaat
     8701 ttaaagagat caaggaagct tatgaagttc tgaccgactc gcaaaaacgt gcggcatacg
     8761 atcagtatgg tcatgctgcg tttgagcaag gcggtatggg cggcggcggt tttggcggcg
     8821 gcgcagactt cagcgatatt tttggtgacg ttttcggcga tatttttggc ggcggacgtg
     8881 gtcgtcaacg tgcggcgcgc ggtgctgatt tacgctataa catggagctc accctcgaag
     8941 aagctgtacg tggcgtgacc aaagagatcc gcattccgac tctggaagag tgtgacgttt
     9001 gccacggtag cggtgcaaaa ccaggtacac agccgcagac ctgtccgacc tgtcatggtt
     9061 ctggtcaggt gcagatgcgt cagggattct tcgctgtaca gcagacctgt ccacactgtc
     9121 agggccgcgg tacgctgatc aaagatccgt gcaacaaatg tcatggtcat ggtcgtgttg
     9181 agcgcagcaa aacgctgtcc gttaaaatcc cggcaggggt ggacactgga gaccgcatcc
     9241 gtcttgcggg cgaaggtgaa gcgggcgagc atggcgcacc ggcaggcgat ctgtacgttc
     9301 aggttcaggt taaacagcac ccgattttcg agcgtgaagg caacaacctc tattgcgaag
     9361 tcccgatcaa cttcgctatg gcggcgctgg gtggcgaaat cgaagtaccg acccttgatg
     9421 gtcgcgtcaa actgaaagtg cctggcgaaa cccagaccgg taagcttttc cgtatgcgcg
     9481 gtaaaggcgt caagtctgtc cgcggtggcg cacagggtga tttgctgtgc cgcgttgtcg
     9541 tcgaaacacc ggtaggcctg aacgagaagc agaaacagct gctgcaagag ctgcaagaaa
     9601 gcttcggtgg cccaaccggt gagcacaaca gcccgcgctc aaagagcttc tttgatggtg
     9661 tgaagaagtt ttttgacgac ctgacccgct aacctcccca aaagcctgcc cgtgggcagg
     9721 cttgggtaaa attagggtgc gttgaagata tgcgagcacc tgaaaagtgg cggggatcac
     9781 tccccgccgt tgcacttact cggattcgta agccgtgaaa acagcaacct ccgtctggcc
     9841 agttcggatg tgaacctcac agaggtcttt tctcgttacc agcgccgcca ctacggcggt
     9901 gatacagatg acgatcaggg cgacaatcat cgccttatgc tgcttcattg ctctcttctc
     9961 cttgacctta cggtcagtaa gaggcactct acatgtgttc agcatatagg gggcctcggg
    10021 ttgatagtaa aatatcactc ggggcttttc tctatctgcc gttcagctaa tgcctgagac
    10081 agatagcctc aagcacccgc cgctattata tcgctctctt taacccattc tgttttatcg
    10141 attctaatcc tgaagacgcc tcgcattttt gtggcgtaat tttttaatga t
//
LOCUS       DAZGXZ010000169        10126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000169 DAZGXZ010000000
VERSION     DAZGXZ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.169"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1108
                     /locus_tag="ACHKJF_004584"
     CDS             <1..1108
                     /locus_tag="ACHKJF_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0951828.1"
                     /translation="AYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTLAVSI
                     AMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVILVGIK
                     MMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADKVKDP
                     QVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHVGYVI
                     VVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVVLIML
                     MTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVIVGFI
                     YSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            1139..1236
                     /locus_tag="ACHKJF_004585"
                     /pseudo
     CDS             1139..1236
                     /locus_tag="ACHKJF_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1233..1640
                     /gene="cueR"
                     /locus_tag="ACHKJF_004586"
     CDS             1233..1640
                     /gene="cueR"
                     /locus_tag="ACHKJF_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP0951829.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(1641..2099)
                     /gene="ybbJ"
                     /locus_tag="ACHKJF_004587"
     CDS             complement(1641..2099)
                     /gene="ybbJ"
                     /locus_tag="ACHKJF_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP0951830.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(2096..3013)
                     /gene="qmcA"
                     /locus_tag="ACHKJF_004588"
     CDS             complement(2096..3013)
                     /gene="qmcA"
                     /locus_tag="ACHKJF_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0951831.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            3159..3836
                     /gene="fetA"
                     /locus_tag="ACHKJF_004589"
     CDS             3159..3836
                     /gene="fetA"
                     /locus_tag="ACHKJF_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP0951832.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            3823..4602
                     /gene="fetB"
                     /locus_tag="ACHKJF_004590"
     CDS             3823..4602
                     /gene="fetB"
                     /locus_tag="ACHKJF_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP0951833.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(4665..5519)
                     /gene="cnoX"
                     /locus_tag="ACHKJF_004591"
     CDS             complement(4665..5519)
                     /gene="cnoX"
                     /locus_tag="ACHKJF_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP0951834.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(5580..6389)
                     /gene="ybbO"
                     /locus_tag="ACHKJF_004592"
     CDS             complement(5580..6389)
                     /gene="ybbO"
                     /locus_tag="ACHKJF_004592"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0951835.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(6379..7002)
                     /gene="tesA"
                     /locus_tag="ACHKJF_004593"
     CDS             complement(6379..7002)
                     /gene="tesA"
                     /locus_tag="ACHKJF_004593"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP0951836.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6973..7659
                     /gene="ybbA"
                     /locus_tag="ACHKJF_004594"
     CDS             6973..7659
                     /gene="ybbA"
                     /locus_tag="ACHKJF_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP0951837.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            7656..10070
                     /gene="ybbP"
                     /locus_tag="ACHKJF_004595"
     CDS             7656..10070
                     /gene="ybbP"
                     /locus_tag="ACHKJF_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP0951838.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLLVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMMLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         2223 a         2611 c         2830 g         2462 t
ORIGIN      
        1 tgcctatgcg cgtctggggg cgagctatcc cagcaatggc ggcattatcg acttctttcg
       61 tcgcggatta ggcaacggcg tcttttcgct ggcgctctcg ttactgtacc tgttgacgct
      121 ggcggtgagc atcgccatgg tcgcccgtgc ttttggcgct tatgccgtgc agtttttgca
      181 tgaaggcagc caggaggagc accttatttt gctctacgcg ttggggatca ttgcggtgat
      241 gacgcttttc aactccttaa gcaaccatgc ggtagggcgg ctggaagtga tcctcgtcgg
      301 cattaaaatg atgatcctgt tattgctgat tattgccggt gtctggtcgc tgcaaccagc
      361 gcatatttcc gtctctgcgc cccccagctc cggtgcgttc ttctcctgta ttgggataac
      421 tttccttgcc tatgcgggct ttggcatgat ggcgaacgcg gcggataaag tgaaagatcc
      481 gcaggtcatt atgccacggg cgtttctggt ggcgattggc gttaccacgt tgctttatat
      541 ctcgctggca ctggttttgc ttagcgatgt atcggcatta gagttagaaa aatatgccga
      601 taccgccgta gcgcaggctg cttctccgct gctcgggcat gtgggttatg tgatcgtcgt
      661 tatcggcgct ttactggcga cggcttcagc cattaacgcg aacctgttcg ccgtgtttaa
      721 catcatggac aacatgggca gcgaacgcga actgccgaag ctaatgaata aatccctgtg
      781 gcggcagagt acctggggca acattattgt cgtggtgttg attatgctga tgacggcggc
      841 gctaaattta ggctcactcg ccagtgttgc cagcgccacc tttttgattt gctacctggc
      901 ggtgtttgtg gtggcgatcc gcctgcgtca tgatattcac gcctcgttgc cgattcttat
      961 cgttggtacg ttggtgatgt tgttggtgat cgttggcttt atctacagtc tgtggtccca
     1021 gggtagccgt gcgttgatat ggattattgg ctcactctta ctcagcctta ttgtggcaat
     1081 ggtcatgaag cgcaataaaa ccgtataaca tctctctgtg cgcagtactt cctgtattat
     1141 tgtggtggcg gtcgatattc gcactggcaa aaaaacgtgc ttgaatatct gttgaaaccc
     1201 tttaacaaag cacaggaggc gttgcgcgaa cgatgaacat cagcgatgta gcaaaaatta
     1261 ccggcctgac cagcaaagcc attcgcttct atgaagagaa ggggctggtg acgccgccga
     1321 tgcgcagcga aaacggttat cgcacctaca cgcagcagca tctcaacgaa ctgaccttac
     1381 tgcgccaggc acggcaggtg ggctttaacc tggaagagag cggcgagctg gtgaatctgt
     1441 ttaacgaccc gcagcggcac agcgccgacg tcaaacgccg cacgctggag aaggtggcgg
     1501 agatcgaacg acacattgag gagctgcaat ccatgcgcga ccagctgctg gcactggcga
     1561 atgcctgccc tggcgatgac agcgccgatt gcccgattat cgaaaatctc tccggctgct
     1621 gtcatcatcg ggcagggtga ttaagacgag actgctcgga tatgtagggt tatcccttct
     1681 atcgcaatga cttcaacatg cgtacctgca ccgagatcct cgctggcgct gacaggccat
     1741 gaactgtcac cgacgcgcat atgaccgcgc ccgttgacca gcggagattc cagcacaaaa
     1801 cgtcggccaa tcagctgctg cccgcgctgg tttaaatgac tatcgctgtg cttttgttcg
     1861 cgcacccgcc gcgacaacca tttccaccac agccaggcgg cgagcagcgt caggatggca
     1921 aacattaccc cttgccactc ccaacccagc ggcaccagcc agaccaccag gccggtaatc
     1981 accgctgcca cgccgctcca caacaaataa ccatttccgc ccagcatctc ggctgccagc
     2041 agcaaaccgc cgagactgag ccagaaaata tgtggatgaa cgaccattaa ctccatcatg
     2101 gctgagtccg tttgttggcg ctgtctttca ccagctcggc aatcccggca atcgacccca
     2161 tcaggctgct ggcctctaat ggcatcatca ctactttgct gttactggag gaaccgatct
     2221 gctgtaacgc ttcggtgtat ttctgcgcta cgaagtagtt caccgcctga atatcaccgg
     2281 aggcgatagc ttcagacacc attttggtgg cgcgggcttc tgcttcggcg gaacgttcac
     2341 gcgcttcagc ctgtaaaaac gccgactgac gttcgccttc cgctttcagg atttgcgact
     2401 gtttttcacc ttcggctttg aggatttccg cctgacggat cccttccgct tcaagaatgt
     2461 aagcgcgttt ggtacgttcc gctttcatct gcgcgttcat tgaagagata agctctgccg
     2521 gtgggcgcac gtcgcgaatt tcaatacggg tgactttaat cccccacggg ttggtggctt
     2581 catcgacaat acgcagcagg cgtgagttga tgctgtcgcg ctgagagagc atttcgtcaa
     2641 gttccattga acccaacacg gtacggatgt tagtcatggt caggttgatg atcgccagct
     2701 ccagattgct gacttcataa gccgcgcgcg gcgcgtcaat cacctgaata aaacagactg
     2761 cgtcgatggt aacgttggcg ttatctttcg agataacttc ctgggaaggg atatcgagca
     2821 cttgctccat catattgatc ttgcgaccaa tgcgatccat aaacggcacc accagactga
     2881 gccccggctg taacgttttg gtatagcgac caaaacgttc taccgtccat tgatagccct
     2941 gcggcacgat tttgacgccc gcgccgacaa tgaccagcgc gacaaaaatg agaatcggga
     3001 taaagataag catcggaaaa acctcctgtt gtaccgtcca taatcagcaa aattgctgct
     3061 tgattaaaca aattatacct gattactgaa agagagttcc cccttattcc tgcgaaggat
     3121 aaactgtttt tagtaaaaat cagaaaaagg gaacagtgat gcaggaaaat agtcctttgc
     3181 ttcagctaca aaacgtagga tatctggcgg gtgatgcgaa gattcttaat aacatcaatt
     3241 tttcgctgcg tgctggcgaa tttaagttaa ttaccggtcc ttccggttgt ggcaaaagta
     3301 cgctgctaaa aatagttgct tcattgatca gcccaaccag cggaacgtta ctgtttgaag
     3361 gtgaggatgt cagcacacta aagccagaaa tctaccgcca gcaagtctct tactgcgccc
     3421 agacgccgac gctgtttggc gatacggtat acgataatct gatctttccc tggcagatcc
     3481 gtaaccagca gcctgacccg gccatttttc tcgattttct cgaacgcttc gctttgccgg
     3541 acagcatttt gacgaaaaat atcgccgagc tatctggtgg tgaaaaacaa cgcatctcac
     3601 tgattcgtaa cctgcaattt atgccgaagg ttttattgct ggatgaaatc accagtgcgc
     3661 tggatgaaag taataaacat aacgttaatg agatgatcca tcgttatgtg cgcgagcaaa
     3721 atattgccgt gctgtgggtg acacacgata aagacgaaat taatcatgcg gataaagtga
     3781 ttacactgca accgcatgcc ggagaaatgc aggaagcacg ctatgaactc gcataatatt
     3841 actaacgaat cattggcact ggcattaatg ctggtggtgg tggcaatctt aattagccat
     3901 aaagaaaaac tggcgctgga gaaagatatt ttctggagcg tcgggcgagc gataattcag
     3961 ctgattattg tcggctatgt gctgaagtat attttcagcg tggatgatgc cagcctgaca
     4021 ttattgatgg tgttatttat ctgctttaat gcggcgtgga acgcgcaaaa acgcagtaaa
     4081 tatattgcta aagcttttat ctcatcgttt attgccatta cggtcggggc gggaattacc
     4141 ctggcggtgc tgattctctc tgggtcgatt gaatttatcc cgatgcaggt gatccctatc
     4201 gccgggatga ttgccggtaa cgccatggta gcggtggggt tgtgttacaa caatttaggg
     4261 caacgggtca ttagcgaaca gcaacagatt caggagaaac tgagtcttgg tgcgacgccg
     4321 aagcaggctt cagcgatatt gattcgcgac agtattcgcg cggctttaat tccgacggtc
     4381 gattcagcaa aaacggttgg cttagtgagt ttaccaggaa tgatgtccgg gctgatattt
     4441 gccgggattg atccggtgaa ggcgattaaa tatcagatta tggtgacctt tatgctgctc
     4501 tcaaccgcca gcttgtcgac cattattgcc tgctatttaa cctatcgtaa gttttataat
     4561 tcgcgccacc agttggtggt gacgcaattg aagaagaaat gatgcgatgc cggatgcgca
     4621 gcatcgcatc cggcgttgtg gttcatgtgc cgatcaacgg aatatcaata caacaatgca
     4681 tacagctggc ggcgatactt cgacgccagt gcatcacccg tacccagcgc agcgaggatc
     4741 tcctggaacg ttttacgcgt ctgaccgtct gcggcggtga gatctttacg cagatgcccg
     4801 aacagcaact ccagcgcctc ttcattgcgc ccaacctggt gcagttgcag agccagttgc
     4861 gtcgccagtg cggcatcttc tggattctcc gccacctgct gttgcaactg ttgaatttcc
     4921 ggcgtatcag ccgcctgctt cagcagttcg atttgcgcca ccagcccctg gtagcgggtg
     4981 tcctgatcct gcaacggaat ggttttcagc accgcttccg catcttcaga acggttcagc
     5041 gcaatcagcg tttctgccag cagcaggccg atctccccgt tctgattcga caactgccag
     5101 gcgtctttca gcaatggcag ggcatcggtg taattgcctt cctgcatcag ttgcatcgcc
     5161 tgctgcgctt tcagctcttc ttcgcgcggc agcactttat ccagcagggc gcggatcgcc
     5221 tcttccggtt gcggcccctg gaagccatct accggttgcc cgttctggaa cagatacacg
     5281 gtcggaatcg cgcgcagacc aaactgggcg gcaatcatct gctccgcgtc gcagtccagc
     5341 ttcgccagaa taaattgccc gttgtactgc gccgcgaggc tttccaggat cggggttaac
     5401 tgcaaacagt gctggctacg ttcagaccaa aaatagaaca gcaccggagt ggtcatcgac
     5461 tgttcaagaa cctgttgcag gttagattcg ttaatgttga caatattttc tacggacatg
     5521 gagtcgctct ctgttgtcga tattttcttt gacatggggg cttaagcgcg cgcttcaact
     5581 cacccctgca atattttgtc catcacgcgc cccggcagca ggcgcttgag caccattact
     5641 gcccaggtca ccagcgttac cggatagcgc atcttcggct tctcgctaat aaaagcatgg
     5701 cgtactttgt ccaccaccgc ttccggtccc aacgtaaagc gggcggcgat gccgggattt
     5761 tcgactggtt tatcactttg cgtctggttg acgttgtcgg tgaagcgagt acgaatggga
     5821 ccgggttcga tcaggctgac tttaattccg ctgtggcgca gctccatgcg cagtgcgtct
     5881 gaccacgcct ccagcgcata tttactggcc gcgtaagcgc cacgacccgg cgtggagatt
     5941 aatcccatca ccgatgatgt catcacaata cgcccttcac cgtgcggtaa catcgcgggt
     6001 aacaggcgca tggtgagctg gtgtgcgccg aaaaagttgg cggaaaactg ctgttccatc
     6061 tgcgcacggc tgatggtgga aagggggcca tacatgccga atccggcatt gttaaagatc
     6121 ccatacagac aattatcggt cagggcgatc acctcgtctg ctgcgcgatc aacactttct
     6181 ggtgaatcca gatcgatcaa cacgccggta aatcccatgt tgttcatacg ctcaacatca
     6241 tccggttttc ggcaacctgc cagcacatga aaaccctggc gttttaattc gagcgcgctt
     6301 tccaggccaa ttccactgga acatccggta attaagaccg atttttgcat aactttacct
     6361 gtcaggatct ccgttgcttt atgagtcatg atttactaaa ggctgcaact gcttcgccat
     6421 ccagtcggca ataaacggct gggcgtcgcg gttgggatga ataccgtcat cctgcatcca
     6481 ttgtggcttg aggtagacct cttccataaa aaagggcagc agcggaacat caaactcttt
     6541 ggcgagtttg gggtaaatgg cgctaaaggc ttcattataa cggcgaccat agtttgcagg
     6601 cagacgtatt tgcattaaca atggttcagc gttggcggct ttgacatcct gcaaaatctg
     6661 gcgcagcgtt tgctcggttt gctgtggctg aaaaccacgc aaaccgtcat tgccgcccag
     6721 ttcaaccagc acccaacgcg gctgatgctg tttcagcaga gccggaaggc gcgccagtcc
     6781 ttgttgcgag gtgtcgccgc tgatgctggc attaactacc gacgttttac tctgccactt
     6841 atcattcaac aaggcaggcc aggccgcgct ggcagacatt cgatacccgg cgctcaggct
     6901 atcacccaga atcaataacg tgtccgctgc ggcggcacgg aaggttaaca ggaccaggaa
     6961 caggaagggc aaatgccagc ggaaaacatt gttgaagttc atcatcttaa gaagtccgtc
     7021 ggtcaggggg agcatgaact ctccatcctc accggagttg agctggttgt caaacgtggc
     7081 gagaccatcg cactggtggg cgagtcggga tcgggtaagt caaccttgct ggcgatcctc
     7141 gccgggctgg atgacggcag cagtggcgaa gtgagtctgg tgggacaacc gctacataat
     7201 atggacgaag aagcgcgggc aaagttgcgc gcgaagcacg tcggctttgt ttttcagtca
     7261 tttatgttaa ttcctaccct taacgcgctg gaaaacgtcg agcttccggc gctgctgcgc
     7321 ggtgagagta gcgcggaaag tcgtaacgga gcgaaagcgt tgctcgaaca gttagggctg
     7381 ggtaaacgtc ttgatcatct tccggcacag ctttccggcg gcgaacagca acgagtggcg
     7441 ctggcgcgag cctttaatgg tcgacctgat gtgctgtttg ccgacgaacc caccggcaac
     7501 cttgaccgcc agacgggcga taaaattgcc gacttgctgt tttccctcaa ccgtgagcat
     7561 ggcaccacgt tgattatggt gacccacgac ctgcaactgg cggcacgctg cgaccgctgc
     7621 ttacggctgg tgaacgggca gttgcaggag gaagcatgat tgcacgttgg ttctggcgcg
     7681 aatggcgttc gccgtcgcta ttaattgtct ggctggcgct aagcctggcg gtggcctgcg
     7741 tgctggcgct gggcaatatc agcgatcgca tggagaaggg cttaagccaa caaagccggg
     7801 agtttatggc gggcgatcgg gcgttgcgca gttcgcgcga agtgccgcaa gcatggctgg
     7861 aggaagcgca aaagcgcggc ctgaaagtcg gcaagcagct gactttcgcc accatgacat
     7921 ttgcaggcga cacaccgcag ctggcgaacg tcaaagcggt ggatgatatc tacccgatgt
     7981 atggcgatct gcaaactaat ccccctggcc tgaaaccgca ggcgggcagc gtattgctgg
     8041 ccccacgcct gatggcgctg cttaacctga aaacgggcga caccattgac gtgggcgatg
     8101 ccaccttgcg gattgccgga gaagtgattc aggaaccgga ttccggtttt aaccccttcc
     8161 agatggctcc gcgtctgatg atgaatctgg cggatgtcga taaaaccgga gccgtgcagc
     8221 cggggagtcg ggtcacctgg cgttataaat tcggcggcaa cgagaaccag ctcgacggct
     8281 atgagaaatg gttgttaccc cagcttaaac ccgaacaacg ctggtacggt ctggaacagg
     8341 acgaaggcgc gctggggcga tcgatggaac gctcgcaaca gttcctgctg ctttcggcgc
     8401 ttctgacctt gctgctggca gtggcagcag tggcggtagc gatgaatcat tactgtcgca
     8461 gtcgctacga tctggtggcg atcctcaaaa cgctgggggc agggcgagcg caactgcgta
     8521 agctaatcgt cggtcagtgg ttgctggtgc tggcgctttc agccgttacc ggtggggcga
     8581 taggcctgtt gttcgaaaac gtgttgatgg tgctgctcaa gcccgttctg cctgctgcac
     8641 taccgccagc cagcctctgg ccgtggctat gggcgctggg caccatgacg gttatctcgc
     8701 tgctggtggg gctacgaccg taccgcttgt tgctggcaac gcagccttta cgcgtattac
     8761 gtaatgatgt ggtcgcgaac gtctggccgc tgaagtttta tctgccgatt gtcagtgtgg
     8821 tggttgtgct gctgctcgcc ggattaatgg gtggcagcat gctgctttgg gcggtgctgg
     8881 cgggcgcggt agtactggct ttgctgtgcg gtgtgctggg ctggatgatg ctgaatgtac
     8941 ttcgccgcat gacgctgaaa tcgctacctc tgcgcctggc ggttagccgc ctgttacgtc
     9001 agccgtggtc aacattaagc cagctttcgg cattttcgct ctcctttatg ctgctggcgc
     9061 tgttgctggt attgcgtggc gatctgcttg accgctggca gcagcagcta cctccagaaa
     9121 gcccgaacta ctttttaatt aacattgcca cagaacaggt agaaccgcta aaagcgttcc
     9181 tcgcggaaca tcagatagtc ccggaatcat tttatccggt agtgcgggcg cggctgacgg
     9241 cgattaacga taagccgaca gaaggtaatg aagatgaggc gcttaaccgc gaactcaatc
     9301 ttacctggca aaatacgcga cccgatcata atccgattgt cgccggtaac tggccgccaa
     9361 aagccgatga agtgtcgatg gaagaggggc tggcgaaacg cttaaacgtt gccctcggtg
     9421 ataccgtgac ttttatgggc gatacccagg agttccgcgc taaagtgacc agcctgcgca
     9481 aagtggactg ggaaagtctg cggcctaatt tctattttat tttccctgaa ggggcattag
     9541 acgggcaacc gcagagctgg cttaccagtt tccgctggga gaatggcaac ggcatgttga
     9601 cacaactcaa ccgccagttc ccgaccatta gtctgttaga tattggcgcg attttaaaac
     9661 aggtcggtca ggtgctggag caggtaagtc gggcgctgga agtgatggtg gtactggtca
     9721 ccgcctgcgg tatgttgctg ttgctggctc aggtgcaggt gggaatgcgt cagcgtcatc
     9781 aggagctggt ggtgtggcgc acactcggtg cggggaaaaa gctgctgcgc acgacattgt
     9841 ggtgtgagtt cgccatgctt gggtttgttt ctggcctggt agccgcaatt ggtgcggaaa
     9901 cggcgctggc agtgttgcag gcgaaagtgt ttgattttcc gtgggagcca gactggcgat
     9961 tgtggattgt tctgccgtgc agcggtgcgc tgctgctgtc gcttttcggc ggctggctgg
    10021 gtgcgcgact ggttaagggt aaggcgctgt tcaggcagtt tgcggggtga tgagagtaaa
    10081 taaggggcgt tctggttgaa tggaacgcct tgtttaaatt aattct
//
LOCUS       DAZGXZ010000170         9988 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000170 DAZGXZ010000000
VERSION     DAZGXZ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.170"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(297..372)
                     /locus_tag="ACHKJF_004596"
     tRNA            complement(297..372)
                     /locus_tag="ACHKJF_004596"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(337..339),aa:Asn,seq:gtt)
     gene            complement(473..1960)
                     /gene="yeeO"
                     /locus_tag="ACHKJF_004597"
     CDS             complement(473..1960)
                     /gene="yeeO"
                     /locus_tag="ACHKJF_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP0951839.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            2121..2196
                     /locus_tag="ACHKJF_004598"
     tRNA            2121..2196
                     /locus_tag="ACHKJF_004598"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2154..2156,aa:Asn,seq:gtt)
     gene            complement(2234..3184)
                     /gene="cbl"
                     /locus_tag="ACHKJF_004599"
     CDS             complement(2234..3184)
                     /gene="cbl"
                     /locus_tag="ACHKJF_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP0951840.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEKNLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(3286..4203)
                     /gene="nac"
                     /locus_tag="ACHKJF_004600"
     CDS             complement(3286..4203)
                     /gene="nac"
                     /locus_tag="ACHKJF_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP0951841.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4529..4604
                     /locus_tag="ACHKJF_004601"
     tRNA            4529..4604
                     /locus_tag="ACHKJF_004601"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4562..4564,aa:Asn,seq:gtt)
     gene            complement(4660..5595)
                     /gene="ldtA"
                     /locus_tag="ACHKJF_004602"
     CDS             complement(4660..5595)
                     /gene="ldtA"
                     /locus_tag="ACHKJF_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0951842.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5657..6736)
                     /gene="cobT"
                     /locus_tag="ACHKJF_004603"
     CDS             complement(5657..6736)
                     /gene="cobT"
                     /locus_tag="ACHKJF_004603"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP0951843.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6748..7491)
                     /gene="cobS"
                     /locus_tag="ACHKJF_004604"
     CDS             complement(6748..7491)
                     /gene="cobS"
                     /locus_tag="ACHKJF_004604"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP0951844.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7488..8033)
                     /gene="cobU"
                     /locus_tag="ACHKJF_004605"
     CDS             complement(7488..8033)
                     /gene="cobU"
                     /locus_tag="ACHKJF_004605"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP0951845.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(8185..8358)
                     /locus_tag="ACHKJF_004606"
                     /pseudo
     CDS             complement(8185..8358)
                     /locus_tag="ACHKJF_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(8337..8525)
                     /locus_tag="ACHKJF_004607"
                     /pseudo
     CDS             complement(8337..8525)
                     /locus_tag="ACHKJF_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            9739..>9988
                     /locus_tag="ACHKJF_004608"
     CDS             9739..>9988
                     /locus_tag="ACHKJF_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0951846.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDILKKA"
BASE COUNT         2800 a         2621 c         2129 g         2438 t
ORIGIN      
        1 gaagacaaag tggcaattac gtatagtttc ccgataaaga cgcgacagct acatcccgca
       61 taaggcattt ttctctttat ctttgtacgg tacttcatgg aacagaattt ttgaccttgt
      121 gaatcgtgat gtctgttggg gaggagcaat ttgctcactg aagcgtgaga ctcgattaag
      181 cgcacgaaac acagaaatca aaaaacccgg ccactttttt acaaactgac cgggtaaaaa
      241 taatttttat tttttaactg ccttgagact catagagatg tctcaaaact aaaatttggc
      301 tcctctgact ggactcgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
      361 aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca caatgtcaaa
      421 gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtt gtttattccg
      481 acacaactgg ctttttttca cacttttgcg gttcgggtcg agggtatttc catagccaac
      541 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc
      601 ccatccagac accaaccaca ccccagccaa gcatgatgcc cagcacataa ccgacgacaa
      661 cccggcaacc ccacatgctc aacatagaaa cccacatagc gtaacgggcg tcacgagccc
      721 ccttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat aaagcattta
      781 gccaaatcag aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca
      841 taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggacagcc
      901 agaagacatg ccgcaactga atctctgctt gcgctatctg ccctacccct aaccttcggc
      961 ctgtaatgat cgtagaagca gagccgagcg cacttccggg taagttgata agagccgcaa
     1021 ttgaaaacgc gataaaattt ccggcaataa cactggtccc catcccggca acgaacattt
     1081 gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgctgga ataccaatcc
     1141 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
     1201 ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg ccaatataac
     1261 gagaaatggt taaacccaac ccagccccga caaatcccag tcccggccag gagaaaaggc
     1321 cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg ttaatcagta
     1381 gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaatcaaa gtgatggcag
     1441 ctgctggata actgagtacc gtcagctcca gataagtcaa cgccagtgct ttaacttctg
     1501 tcgtggcatc acccgcgacg aaatcaataa tttgctcgcc aaaatgatga ataagcgttg
     1561 ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc
     1621 gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac agtagtacca agatcgatag
     1681 cagcaaaaaa agccataatg accatattga agctgtccgc caatcccacg ccggccatcg
     1741 catcttttcc cagccagctg accagaaaag tgctcagaac ccccatcaac aggacacagg
     1801 cattctccat gaagatagga acagcaagcg gggttatctc gcgccagaac aacactttgt
     1861 agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga
     1921 tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaataat gagggagaat
     1981 cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata
     2041 gaaacggcaa acagttgggg aatttaaaaa tcgggtttga caaaagattt ttcgccgtta
     2101 agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     2161 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt tctgaaaagc ccgcttttat
     2221 agcgggcctt ttgctatatc tgataatcaa tttcctcttc actgttttcc atcacctggc
     2281 gcttgatatc ctcaactgac agtcctgcat tacaaagttc cagaaagcgc cagacatagt
     2341 tacgctgaag ttgtcctcgc ttgagtccca accagacagt attagcatcg aaaagatgcc
     2401 gcgtatccag gcggattaaa ttcttttcct cttgttcacc gctggattgt tcggcaacta
     2461 atccgatccc aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgcgcactta
     2521 atacaatatc tgccagtaaa cctttgcggg caaatgcgtc atcaatacgt gagcgccccg
     2581 taatcccctg tcggtaagtg attaacggcc acttcgctat tgattccagc gtcaatggtg
     2641 taatttgcgt caagggatga tcgtgtggaa caagcaaact atggtgccaa cgaaaccacg
     2701 ggaaggcgac gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt
     2761 cgccattttg caacaacgtc gcaatttcct gtggcgtacc ctggattagc tccagccgaa
     2821 cctccgggaa aagttcgcga aaagctttaa tgacctcagg caagctataa cgtgcctgag
     2881 tatgcgttgt tgcaatagtg agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc
     2941 gacgaacatt actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg
     3001 gttcagtcat gcccagcagt cgcttacctc gtcgaacaaa tatttcgatg ccaagttcat
     3061 cctccagttc ccgaatatga cggctgacgc cggactgtga ggtaaaaagc atattcgcaa
     3121 cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat
     3181 tcacggtaaa ctccgggcag ctcagatttc ccgttattgt taaagtctaa tgcccggcat
     3241 aacaaataat aaaaacccgc atcttattcc atcccgatat aacgcttagc tcaccaattg
     3301 ccactgcctt ttttccatca ctggagaact aatcactgac attaacaact ctttcactgc
     3361 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
     3421 ggaaggcgtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
     3481 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
     3541 agactcaatt tcaccaatga cttttgccgt gagccgccgt agggaaaaag cctcatcaac
     3601 acgaagtcta acagcactgt aatcactggg gagaaagagg ttcatttgcg caatagcatt
     3661 aacatcaacg ctttgccccg ggcaatcttg agttcctacc aggaaaagat cttctttcag
     3721 caaagcctga ctggataccc cagccacagg ggaatgctca taaatcaccg ccatatcgag
     3781 ttggtgattt atcagttttt cgttaagcac tgcaccgcta ttttcatgaa gatagataac
     3841 gatctccggg aattcagcgc gaaccgcctg taataagggc atggtgatag atgacgcagc
     3901 ggttcctggc gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
     3961 cgccagttgg gcctgctcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
     4021 tccggcgtct gttggcgtaa cgccccgttt tgtgcgaatc aaaagttgtt gatttaactc
     4081 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
     4141 agcctgagtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
     4201 catgttgcct ccggtttttg agaatcggcc caagtgccgc cattacttac aaccagactg
     4261 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     4321 agattatgat agattcgtca ttggctcttt tcaccagcag tcgcgttccc ctgccctata
     4381 aaaggagggt atgcaccacg atggttcatt accaataaga ttgaaatctc atcactttgt
     4441 tgaaattggc agcaaacaaa caaaaaaatg catttcaccc tttgacatca ccatgcactg
     4501 ccattaatat gcgccccgtt cacacgattc ctctgtagtt cagtcggtag aacggcggac
     4561 tgttaatccg tatgtcactg gttcgagtcc agtcagagga gccaaattca aaaaagcctg
     4621 ctttctagca ggctttttgc tttctaatta ccaacgctct taaaacatct gtcttgaacc
     4681 agaactaatt tgcacaggca ttcccgatcg acgttgcaac gcagcatttg cgcgattcac
     4741 atcaacttct tgcccgttga taaacgcccg caaagatggg gttaccggca atggcacttt
     4801 tcggtcagac tcatattctg cacgattgcg cgacagcggc tcatgaactt ccagccagtt
     4861 cgagccatct ggttcagtgg tgtattttac tggctggtcg ataatttgca cacgcgttcc
     4921 aacaggaaca ttatcaaaca gatatttgat atcgtcattg cgcagacgaa tacagccctg
     4981 acttacccga agtccaatac caaaattggc attggtaccg tggatggcat acaacctgcc
     5041 aatataaatc gcgtacagcc ccatgggatt atcgggcccc gcaggaacaa atgcgggcaa
     5101 actctcccct cgtttcgcat attcgcgccg agtgttcggc gttggcgtcc aggttggagc
     5161 ttcttgttta cgttcaacgg tagtcaccca gttacgcggg gtttctcgcc cagcctggcc
     5221 gataccaata ggaaagactt ccacagtatt actgtctggc gggtagtaat aaagacgcat
     5281 ctcagcgacg ttaaccacaa tccctttacg aacagtggcg ggcaaaatca gttgctgcgg
     5341 aatggtgagt tgcgagccag acttcggcaa gaaaacatca gcgcccggat tcgcttccag
     5401 catgttactt aacccttgcc cgtattgtgc ggcaaaagtc tccagcggct gggtattgtg
     5461 atcaggaaca gttacagtaa gcgactgccc caccaaacgg ctaccctctg gaggtaatgg
     5521 ataagttacc gccaggctag tatggctggc aaaaagcaga gcaaatgagc aaagaatatt
     5581 tacacgacgc atcatgtccc tttcctatgt cgcgaaagct atccgttaag tatagctttt
     5641 atcagacttt tcgtttttaa ctgttcaaat cagaagtcgt attccccggt agaacaatat
     5701 tactggcagc aagttcgccc atgttgttat atatcgcaca ggcagcttcg atgatgggca
     5761 tcgccagagc tgcgccactc ccctcaccta aacgcatctc catattgaga taaggctcca
     5821 gccccaaatg cgacagcgct atacgcgccc ctttttctgc cgacaggtga gaaggaatga
     5881 gatacggttt gattgcagga gacatctggc aggctgcgag cgcagcagca taagaaagaa
     5941 atccatccag caacacgggt aaaccgcagg aagcagcgcc taacatcacg ccagccattc
     6001 cgaccaaatc aaatcccccc acttttgcca gaacatcaac gccatcctgc ggatttggtt
     6061 gattcaacgt aatcgcccgg cgcacaacat caattttatt agccagttta tctgtcggca
     6121 ggtttgcgcc aatcccaacc acttcttcag gatcccggcc agtgattgtg ctgactattg
     6181 ccgctgccgg tgtcgtgttt gccatcccca gttcacctac accaaacagc gtgacaccgt
     6241 tttttgccag ctcccgcgta taacatatga cgtccaaaag caacttttca gcctgacggc
     6301 gactcattgc cggagctgaa gcaatattgc cgctacctcg tgcgacacgc atgttgataa
     6361 gcccggggat aggctcagca ctatcaatac caacatcaac tacgtggacg ttagcgcccg
     6421 cttgtgctgc cagcacacac acgccggttg ttccgcgggt catattttca gcctgaatgg
     6481 ctgtcacttc ttttggggaa atagcgaccc cttcctccca gacgccgtga tcggcacaca
     6541 taaccagtac cgcttttttg cccacatgcg gtatgccatt caaccccggc attcccgcca
     6601 gttgtatggc aagcgcctcc agccttccca ggctaccaac aggtttgagt aacccgtcaa
     6661 tatgccgttg tgcacgcgac atagcggcag gatcgatagc agggatcgta ttcagtaaat
     6721 cggcaagtgt ttgcatgtca cgtctcttta tagcagtgcc agcaggaaga ccagttcacc
     6781 aagctctata gcagctccca gcgtatcgcc ggtttgcccg cccagcgtac gtttgagaag
     6841 ctgaccgagg atgaaaatcg ccaccatcgt caccaccata gcagccacac catgcatacc
     6901 cggcaataat acggcagcaa aaatagcggc taagccgagt gtgacgcagg tttgtcgccc
     6961 atcaattttg ccaatgaaca cattgccaag cccttcttcc cgggcataac gatgacgata
     7021 catcaataat gcggcagtcc cgcggctgac cgcacatgct gccgccagcg atgcaaggat
     7081 cggttcgcca cgcagagcca actcgcttaa taccagaatc tttgccagta caacaaaaat
     7141 caatgccaga ccgccatggg tgcctaaacg gctatcacgc atgatttcca acatgcgatc
     7201 gcggctacgt gcagaaaata cgccatcaca ggtatcggcg aggccgtcca gatggaatcc
     7261 cccggtcatc agcgccagca caagtacgct aaacagtgcc gccagtggta cgccacacca
     7321 tgcctgcagc accatgaaga ccagcccgct aatcgcgcca agcaataatc caattaaagg
     7381 aaaagtaata ataccgcgag aataatgctc gaaatccagt ccctgggacc agcgacgcgg
     7441 tacaggcaga cgcgtaataa atgagagcat cgcccaaaat aatttactca tttgattttt
     7501 actccaatac ccgaaaccac cagccatact tcatttgccg cagcggccag ctgctgattt
     7561 acccgcccgg caatatcacg aaaatgtcgt gccagacgac tctccggcac aatccccatt
     7621 cccacttcgt tagtcactaa tacaaccttt gcggggcaac gttggcaggc agcaatcaac
     7681 gactgaatct cagcattaat cgcctgttcc atcgcctgat aatcccattc atcagggtct
     7741 ttatcgccgc cataatcaaa caacagatta gtcaccattg tggtaacgca ttccagcaac
     7801 acagcttcat gcgggttaat gtctgcatga attaattcat caagatgttg ccaacgctcc
     7861 accgtgcgcc agtgcgccgg acggccttgc cgatgatgtt ctatccgtgc agccatctcg
     7921 tcatcaagga tttgcgaggt agcgatatac agaacctgtg aagagtcccc aataagcgcc
     7981 tctgcgtggc gactcttccc gctccgtgcg ccccccgtca ccaaaatcat cataccgact
     8041 cctgattcaa atgtatgatg gtattcattg aacagaacaa tggatccccc accgttgctg
     8101 accgcaaaac atttacgcga ttaatactgc gcgtaatata attaaaaatc cagcattctc
     8161 aatccattac gaaataagat ggcattacgg aatagccaga ctttctcgtg ttaacgtccg
     8221 acaaaatgcg tcattgacag cggtttctgt ggattgacac atttcattca gataatgaat
     8281 taatgctact gccggaccaa ccagcaaacg ggcattgtgt tctgaaatcc aggctattga
     8341 ttcaacgtca gcgacagatc tgcgctgtaa aagaacttgt aaaacaacgc aatagaatta
     8401 tccagaatgg tggcgactat gcactagaac accacaattt cgctctctcg gtaaggcact
     8461 gtcacagcat tcaacagaat gtgacttgcc agatttatta gcgccaccag atgtttaacc
     8521 gggtaaccac gacccagtcg gggatatatt ccatcttctc gctgacatca tcaagaataa
     8581 aaaggtttat cacactcaga gcaacgttct tcagcaggcg ggataattct tcaacattta
     8641 gaagatgtgt tattaaaggc atacaaactg gacgtattgt attttctttt ggtggttgac
     8701 ctaaggtagc agtttatcct gatgcgctga gatttctgta atatctacgt caacattctc
     8761 ctcgaataga aaatgcagcc ccggcaagtg attcacattt tttaacaaaa cgttgttagc
     8821 gtgccatttt cagaagctct ccaagaaagc gaattctttt ctcaaattct gcattctcag
     8881 acacagcttt ctactccatg gcaatggccc aaatacgaag ttgctcagga tcattagtgg
     8941 tgctgagaag ttaaataacc atgcggagta ccactcgtca tcacgtattt atatatcatg
     9001 aagctattgt tgtgttttgt acatcagtag atatattctg atatactcct tttgctagac
     9061 ataacctttc acctgcttgc aaagcttctg tgttctgaca ttgccaaatt gttgcaattc
     9121 tgtatccagc cttctttcag tcatagcttc gggccgcgat aagactcact gatctgaccc
     9181 tgattcctct tgcagacttt atagaccaat taaaatgcag tttctgcagg tcaacgtctg
     9241 accatcattg tcatcactct ggccattaga gtaaccttct gcattcatcc ttttgtaaaa
     9301 agtttatatc agtatcagca attaaccgga cctgatactg atatgagtct taccgcatat
     9361 acggttaatt tcagcaatta attacattat ccacgccaaa gtatttgtca tcacaatgat
     9421 ggtaccttct ttcagacacc attttttcaa ctccgttttc cacggaccgc actcttatgt
     9481 caagagtgcg gtccgtggat acaaccagag accgactgac acgagtcaga ggaaacgacg
     9541 gatatgttca gtcgtaaaat atctatcaaa aaacatgatt aaggtcaaaa atgtttgata
     9601 tttacaattt atgaagatga caataattat agacacatga gaacataaat gaaaataatt
     9661 atcattacag caatctgaat cgccacgggt ttaacagaca cctcagagtc atttaagatg
     9721 gcttaaagag aggtgcccat gagcggtaag cgttatcccg aagagtttaa aactgaagca
     9781 gtcaaacagg ttgttgatcg cggttattct gttgccagcg ttgcaacacg tctcgatatc
     9841 accacccaca gcctttatgc ctggataaag aagtacggtc cggattcttc cactaataaa
     9901 gaacagtcag atgctcaggc cgagatccgc cgtctccaga aagagctgaa acgggttacc
     9961 gacgaacggg acatattaaa aaaagcca
//
LOCUS       DAZGXZ010000171         9779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000171 DAZGXZ010000000
VERSION     DAZGXZ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.171"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            181..1317
                     /locus_tag="ACHKJF_004609"
     CDS             181..1317
                     /locus_tag="ACHKJF_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0951847.1"
                     /translation="MRKLSMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            1413..3110
                     /locus_tag="ACHKJF_004610"
     CDS             1413..3110
                     /locus_tag="ACHKJF_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP0951848.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLAALMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            3179..3436
                     /locus_tag="ACHKJF_004611"
     CDS             3179..3436
                     /locus_tag="ACHKJF_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951849.1"
                     /translation="MNKHENKKYCSSDEIWCTQRKELIKSVLETKKASDLEKQSTDIA
                     QQYLSETIQFSIDDFIKNIKRKVKSRGSRPPAKRKNTYKGT"
     gene            3657..3872
                     /locus_tag="ACHKJF_004612"
     CDS             3657..3872
                     /locus_tag="ACHKJF_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0951850.1"
                     /translation="MSQQKITGIDLAKTNFYLFSINAHGKPAGRIKLSRNQLLNWLVQ
                     QPKMTVAMEARGHPITGPGKSGNSTMM"
     gene            4046..4303
                     /locus_tag="ACHKJF_004613"
     CDS             4046..4303
                     /locus_tag="ACHKJF_004613"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951851.1"
                     /translation="MERTQLINHVRGLLAEYGIVFSKGATELRQKLPALLEDAENELT
                     DTMKTLLHRQYIRLITLDNELEWYDSELKNMSAKILCANAC"
     gene            4285..4476
                     /locus_tag="ACHKJF_004614"
     CDS             4285..4476
                     /locus_tag="ACHKJF_004614"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0951852.1"
                     /translation="MCKRLLAIPRFSPLVSQAIKSWMGDGKQFRRGRDASATLGLVPR
                     QFSTGGKQVLLGITKFGEM"
     gene            4618..5637
                     /locus_tag="ACHKJF_004615"
     CDS             4618..5637
                     /locus_tag="ACHKJF_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0951853.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDEHTGEVIDKQLRRQDFLMFFCNR
                     EPCLIGMEACRGSQHWGRELTKLGHKVRLLQAHFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKDIAVKTEEQQSVLVLHRSRRQLVKFRTMQINSLHGTLLEFGKTIHKGRAAMEREL
                     PEALERMKERLPPYLITVLENQYNRLNELDSLIEDIEKKLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMREASAFKSGREFAAYVGLVPKQTGSGGKIRLLEISKRGNTYLRAL
                     FIHSARAAALLPKEPGPWITELKKRRPTSVAIVAMANKLARTVWAIAAHGSKYDKNHV
                     SIRSY"
     gene            6358..6573
                     /locus_tag="ACHKJF_004616"
                     /pseudo
     CDS             6358..6573
                     /locus_tag="ACHKJF_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            6748..8718
                     /locus_tag="ACHKJF_004617"
     CDS             6748..8718
                     /locus_tag="ACHKJF_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HFP0951854.1"
                     /translation="MYMNVIRTVICTLIILPVGLQAATSHSSMAKDTITVVATGNQNT
                     VFETPSMVSVITNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVL
                     VLVDGVRQLSDMAKSSGTYLDPALIKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADF
                     LPPGETNGVSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAP
                     NKEKPAALFAKGSVSITDSNKAGASLRLYQNNTTEPGNPTLTHGDNGLRDRKTAQNDV
                     QFWYQYSPADNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFP
                     GAHQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSNPVNIIVGSRYDR
                     YKSFNPRAGKLKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRK
                     GKPNYWVPNLNLKPENNITREIGAGIQLDGLLTDNDRLQLKGGYFGTDARNYIATRVD
                     MKRMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTL
                     ISDISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKSFEAS
                     VTLDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            8737..9528
                     /locus_tag="ACHKJF_004618"
     CDS             8737..9528
                     /locus_tag="ACHKJF_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFP0951855.1"
                     /translation="MMKNTGYILALCLTASEHALAHDVWITGKQAENNVTAEIGYGHN
                     FPSKGTIPDRRNFFENPRLYNGKEIITLKPTSTDYVYKTESAANGNSYVLSTKMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGEQQMPAQLYQSPTGDELEIIPLS
                     DISRFGEDVKLKVLYKTSPLAGATLELDSVSYLKSSRHTHAAEHKHSAHKAELTFVSN
                     EDGIITVPSLHVGQWLAKVKNKKVFPDKSLCDETVDVATLSFSRN"
BASE COUNT         3016 a         2061 c         2232 g         2470 t
ORIGIN      
        1 aggtcaagaa ataacacttg agaaagaaaa gtcaaataca ctagaaaata taaaacctca
       61 aataaaaaca acaatataga tcactgagca atattttgtt acatctttgc atagtgataa
      121 ttttttcgcc atagtttaat ttttaaaacc atactaaaaa tgtgcacagg ggattttttt
      181 atgcgaaaat taagtatggt tgtgtgctct gtcgtttctg cattaatgct gtgtgaggca
      241 acacaggcag cagaaatcta taataaagac ggtaataaac tggattttta tggtcgcgtt
      301 aaagcccgtc attacatctc tgatggttct atggatggcg acagaacatt tgcacgtctt
      361 ggtttcaaag gcgaaacaca aattaatgag agcctgactg gctttggtca gtgggaatac
      421 cagttcctgg ggtttaaaac agaaagtgaa gtacgcaacg ataaaaatcg tctggcattt
      481 gcaggtattc gccacaatat actcggttca cttgattatg ggcgaaacta tggtgttgcc
      541 tatgatgtgg gggctctgac tgacgtactt ccggactttg gtggtgacag ctggacttac
      601 tctgataact atatgaccgg cagaacaacc ggagttctga cctggcgcaa tacagatttc
      661 ttcgggcttg ttgatggact tactttttct gctcaatatc agggtaaaaa tgaccgtgat
      721 aatttacttg aagctaatgg tgatggatat ggtttctccg taagctatga ctatgaaggt
      781 tttggaatcg ttgccgctta cactaatgcc gacagaacca gtctgcagga aaaagaaggc
      841 cgtaaacgtt tcatgacatc gaaagattca gaaggaaaag aagttattac gcctcgctgg
      901 attgccgggg gaaaacatgc ggagtttgca ggtgttgggg tcaagtataa tgataatgac
      961 ctgtatctgg cagctgacta ctctgaaacc cgaaatatga ctccatttgg tagcgcaggc
     1021 atcgcagata aagcacggaa tattgaagtt gtggctcagt atatgtttga tttcggcctg
     1081 cgtccttcac tggcttacgt tcagtccaaa gggatggatg ttaaaggtgg aatagtaaac
     1141 tatggcgatc agtctcttgt tgagtatatt gatgttggcg caaactactt tctgaacaaa
     1201 aacatgtcac ttcttattga ttacaaaatt aaccttattg atgaaagcga atttaccaaa
     1261 aaggctggag ttgcaaccga taatatactt gctgttggta tgatgtatca gttttaacag
     1321 gtatccattt aaacgattta catagttttt atttatatca gaggctttta ttattaataa
     1381 atgttcgtga tgcaactcag aaggaatacc atatggaaca tcagttaaat aaaaaactgc
     1441 ttaactcatc aatatcactt atattagcat ctggtctgat gatgagtact gcaaatgcag
     1501 cagatgatgt taagctcaaa gcaacaaaaa ccaatgttgc ctttgcggac ttcacgccga
     1561 cggaatacag cactaaaggt aagccaaata tcattgtttt aacaatggat gatttagggt
     1621 atggacaatt gccttttgat gatagttcat ttgaccctaa aacgatggat aaccgaaatg
     1681 ttgttgatac atacaaaata ggtattgata aagcaattga agctgcaaaa aaatccactc
     1741 caacattagc ggccctgatg gacgaaggtg taaaattcac taacggctat gttgcacacg
     1801 gtgtctctgg cccttcacgt gccgcaatcc tgacggcgcg agctcctgct cgcttcggtg
     1861 tatattcaaa ttctgatgca caggatggta tccctctgga agaaacattc ctgtctgaat
     1921 tacttcagaa tcatggttat tacactgcag caatcggtaa gtggcactta tcaaaaatca
     1981 gcaacgtagg tgtgcctgaa gaaaaacaaa cccgtgacta ccacgataac ttcaccacct
     2041 tctccgcaga agaatggcaa cctcagaatc gtggcttcaa ctacttcatg ggttatcacg
     2101 catccggcgt ttcactttat aactccccgt cattgttccg taaccgtgaa aacattaaag
     2161 ctgaagggta cattagtgat caattaactg atgaagcaat tggtgtaatt gaccgtgcaa
     2221 gcaaactgga acagccattt atgatttatc tggcatacag tgcaccacac ctgcctaacg
     2281 atcagcctgc gcctaaaaac tatcaggaga aattcaacac tggtagcaag actgctgata
     2341 actattatgc gtcaatttac tccgttgacc agggggttaa aaagatcctg aatacactta
     2401 aggaaaaagg tcagtacgac aacaccatca ttctgttcac ctctgataac ggtgctgtaa
     2461 ttgatggtcc actgccactg aatggtgcgc aggctggtta caaaggccaa ccgtatccgg
     2521 gtggtatgca tacacctatg ttcatgtggt ggaatggcaa gctgaaaacg ggtgattatg
     2581 acaaactggt ttcatcaatg gacttctatc cgaccgcttt agatgccgca gatatcaaga
     2641 tccctgcttc tctgaaacta gatggtgtat cattattgcc atatgtacag aaaggagctg
     2701 ataagaaatc tgcagctggc gaacctcaca aatatctgtc ctggattacc tcttattctc
     2761 actggtggga cgaagagaac actgcattct ggaacggata tcataaattc gtgagaaaag
     2821 agtctgatga ttatccgcac aacccacata cagaagactt aagccagttt gcatacactg
     2881 taagaagcaa cgattattct ctggtttaca cctatgcaga gaataaacta gctctgtacg
     2941 atatcaacga tctgcaacaa actaaagatc tctctgcatc ccatcctgat gtagttaaga
     3001 atatgaaggc tgttatgatt gacttcatca cgtcaagtca aaaaccgatc agtgatatca
     3061 accaagccaa atttgagaat ataaaaaaat ctttaactaa cgtgaaatga taataagcag
     3121 ggagtagata tatctactcc ctggatatag ggaaataaat cagttagagc aataaataat
     3181 gaacaaacac gaaaataaaa aatactgttc atctgacgag atttggtgta cacaacgtaa
     3241 ggagttaatc aaatctgttc ttgaaacaaa gaaagcttct gatttagaaa aacagagtac
     3301 agatatagct caacagtatt tatcagagac gattcagttt tctattgatg attttatcaa
     3361 aaatataaaa agaaaagtta aatcccgagg ttccaggcct cccgccaaac gtaaaaacac
     3421 ttacaaggga acatagattc aatgcgttta tttagaaggt ctgtcttctt tttaatatta
     3481 acttattaat tggtgctatt catataattc tttaaacagc gaacttatag ccaggattca
     3541 atagatatag taacaggaac atcagtaagc gatgagatgg tcttccccct ccttcacgac
     3601 aacctttaat tacgtcatga tggataaact ttaaccacaa aagtaagagg gataccatgt
     3661 ctcaacagaa aatcaccggc atcgatctgg ctaaaacgaa cttttatctt ttcagtatca
     3721 atgctcatgg caaacccgca ggaaggatca aactttctcg caatcaactg cttaactggc
     3781 tggtgcagca gcctaaaatg actgttgcga tggaggctcg cgggcatccc atcactgggc
     3841 cagggaaatc aggaaactcg accatgatgt aatatttctt ccggcgcaac atgttaaagc
     3901 ttatcagcga cgccagaaaa atgattatga cgacgctcag gcaatagctg aggcctgcca
     3961 acatggaact atacgccccg taccaataaa aactctggag taacaggacg tccagacttt
     4021 tttgaaaatg cgccggttgg tttcgatgga aaggacacaa ctgataaatc acgttcgtgg
     4081 acttcttgct gaatatggca ttgtgttcag taaaggcgca acagaattac gccaaaaatt
     4141 accggcatta cttgaggatg cggaaaacga actcactgat accatgaaga cgttattgca
     4201 tcggcagtat atacggttga ttacactaga taatgaactg gagtggtatg attctgaact
     4261 gaaaaatatg tccgccaaga tcctatgtgc aaacgcttgt tagccattcc tagattcagc
     4321 cctctggtta gtcaggcaat aaaaagctgg atgggggatg gaaaacagtt tagacgagga
     4381 agagatgcat ctgcgactct gggactggtt ccccgacagt tcagcacagg tgggaagcag
     4441 gttcttctgg gtataacaaa atttggagaa atgtaatgaa cacatcccac cccattaagt
     4501 caggtgctct gtgatggatg aagggaaata ctggaaggtg tggagcctcg cagatttccg
     4561 gcatcatagt gcccaataca ggatgcataa agctccctca gactggagac tccacaaatg
     4621 aaatatacac cggttggcgt tgatatcgca aaacatctga ttcaggttca tttcatcgac
     4681 gagcacacag gtgaagtgat tgataagcaa ctacgtcgtc aggattttct gatgttcttc
     4741 tgtaatcggg aaccctgcct gattggtatg gaggcctgta gaggctctca gcactggggg
     4801 cgtgaactga caaagctggg gcataaagtc cggttgttac aggcccactt tgttaaggca
     4861 ttcgtcatgg gaaataagaa tgatgtgatg gatgcccggg ctatctggat ggctgttcag
     4921 cagccaggta aggacatcgc ggttaaaaca gaagaacaac agtcagtgct ggttttgcac
     4981 cgttcccgca gacaattggt taagttccgc accatgcaga ttaattctct gcacggcacg
     5041 ctcctagaat ttggtaaaac cattcacaaa ggtcgggcag cgatggagcg agagctccct
     5101 gaagcactgg agcggatgaa agagagactg ccgccgtatc tcattactgt gctggaaaac
     5161 cagtacaacc ggctgaatga gctggactcg ctgatagagg atattgaaaa aaagcttacc
     5221 agcgtggcga gacagaatga aacctgtaag cggttgctgg atattcccgg cgttgggcca
     5281 cttattgcaa cggcagcggt ggccaccatg agggaagcat cagcgtttaa atcagggcga
     5341 gagttcgcgg cttatgttgg tctggtgccc aaacagactg gctcaggagg gaaaatccgc
     5401 ctgctggaga taagcaaacg tggtaataca tatctgcgag ccttgtttat tcatagcgca
     5461 agggcggcag cattactgcc caaagaacct ggtccgtgga taaccgaact gaaaaaacgt
     5521 cgtccaacca gcgtggcaat cgtcgccatg gcaaacaaac tagcacgaac agtatgggcg
     5581 atagcagctc atggaagtaa atacgacaag aatcatgtca gtatcagatc atattaatcg
     5641 ctgataccga taaatattaa cttttaatga aaggatgaat actgaaaggt aactctggcg
     5701 gccatagtga tgacaacgac aggtaagacc gggactcact aaacctgaat attgtgttga
     5761 gctaaaagct cgctggagaa atgaggtgtg agtcagcgaa ttacataggg gctcgcagct
     5821 tcatggctgc aaaaaagccg gatataaagc tgcaacctac ccgtcactgt caaggcaatg
     5881 gatgccttgc aacgggatgt gttcatataa tcaatatgtc agatctatgc tgatacaggg
     5941 tgccagagcg gtattgttca gagcctcaga gaaaactgat tcacttcgtg tgtgggttaa
     6001 ttgtattcgg gaaaagcgtg gcttcaacca ggcagttgtt gctcttgcaa ataaactcct
     6061 tcggatcgcc tgggcaatta tcgcccgcaa tgagatgtat ttgcccgcag caaaacaaaa
     6121 tcaataatct acgtaaaatc agtaggagta aagcttaccc tgaaattgca aagaatgcag
     6181 aatgatgaaa aacaggtcag accgacgtta cgaaaacctg caagatgagc caggccagta
     6241 aggccgagta agcgataagg tcggagcgcg cgaatgtctc atcaaggcca gaagcaataa
     6301 cttcatgaac aaaccggata tacgaaagta aacctgtccc tatcatagtt gctgtactct
     6361 tgcaatacgg gggaagacca tctcatcgct tacgaacaat tcgtgaaaac gatgaagaaa
     6421 gactacatcg cgtgcatgcc gaaatctaat gtaagaacgg cattgcataa tcttgcagtg
     6481 gagatcgggt attacaatga aaaccatccg catagtgcgt taggttatct cccaccaagg
     6541 gaatatcaac gtcagcgaat aacgtcacct tagatgcagt agttgtcagg aaatagtgac
     6601 tcaagggcag gcttaccata tacactctta gtggttcgac tgggtattag aatagctatt
     6661 aacagattag cactgaacac tgcgtaatag tggttgcaca aacaaaatgt tatgttataa
     6721 cattgctgta ttgacatata ttttcgaatg tatatgaacg taatcagaac tgtcatttgt
     6781 acattaatca tacttccggt ggggttacag gcagcgacaa gtcattcttc tatggctaaa
     6841 gatacaatca ccgttgtcgc gacaggaaat cagaacacgg tatttgaaac gccgtcgatg
     6901 gtcagtgtca tcacgaatga cacgccatgg agtcagaatg cggtcacatc agccggtatg
     6961 ctgaaaggtg ttgcgggtct cagccagact ggtgcaggac ggaccaatgg gcagaccttt
     7021 aatctacgcg gctatgacaa aagcggggta cttgttcttg ttgacggcgt ccgtcaactc
     7081 agtgacatgg caaaaagcag tggcacttat ctggatccgg cactcatcaa acgcatcgaa
     7141 gttgtccgcg gaccaaactc cagtctgtac ggcagtggcg ggctgggggg ggtggtggac
     7201 ttcagaactg ccgatgctgc agattttctt ccccccggag agacaaatgg tgtaagtctg
     7261 tggggaaata tcgccagtgg tgaccacagc acaggctcgg ggctcacctg gttcggtaaa
     7321 accggaaaaa cagatgcgct tctttctgtc attatgcgta aaagaggaaa tatctatcag
     7381 agtgatggtg agcgtgcacc taacaaggaa aaacctgcag ccctgtttgc gaaaggctct
     7441 gtcagtataa cagacagtaa caaagcaggt gccagcttgc gtctctacca gaataacacc
     7501 actgaaccgg gcaatcccac tctgacacat ggtgacaacg gcctgcgtga cagaaaaaca
     7561 gcacaaaatg acgtgcagtt ctggtaccag tactcccctg cggataacag cctcatcaat
     7621 gtaaagtcaa cgttatatct cagtgatatc actatcaaga caaacggtca caacaaaaca
     7681 gcagaatggc gaaacaacag aacctccggt gttaatgttg tcaacaggag ccatactctg
     7741 atttttccgg gagcccatca gttaagttat ggcgccgaat attaccgtca gcagcagaag
     7801 ccggaaggct ctgccacact atatccggaa ggaaacattg actttacatc gttgtatttc
     7861 caggatgaaa tgacaatgaa aagcaacccg gttaacatta tcgtcggttc ccgttatgac
     7921 cggtacaaga gcttcaatcc ccgagccgga aaactgaaag ccgaacgcct gtcccccagg
     7981 gcggcgattt cagtctcacc aacagactgg ctgatgatgt acggctccat atcctctgca
     8041 ttccgtgccc ccacaatggc agaaatgtac agggatgatg tacattttta ccgcaagggt
     8101 aaacccaatt actgggtacc taaccttaat ctgaaaccag aaaataacat tacccgtgag
     8161 attggcgcag gtattcaact ggacggcctg cttacagaca atgaccggct gcagttgaaa
     8221 ggcggatatt tcggaacgga tgccagaaac tatattgcca cgcgcgtgga tatgaaacgg
     8281 atgcgttctt attcttataa tgtatcccgg gcccgtatct ggggatggga tatgcagggt
     8341 aattaccagt ctgattattt tgactggatg ctttcttata accgcacgga aagtatggat
     8401 gccagtagca gggaatggct gggctccggc aatcctgaca cactcatcag tgacatcagt
     8461 atacctgttg gtcatggagg agtttctgcc ggatggcgtg ctgaactttc ggcagcagcc
     8521 acgcatgtga aaaaaggcga tccccatcag gctggctatg ccgtacattc cttttcactg
     8581 tcttataagc ctgtaagtgt taaaagcttt gaggcatcag tgactctgga taatgccttc
     8641 aacaagcttg ccatgaatgg caaaggtgtg ccgctttcag gcagaactgt cagtctttat
     8701 acccgttatc agtggtaacc tcgcaggaaa taaatcatga tgaaaaatac aggctatatc
     8761 ttagctcttt gtctgacagc atcggagcat gccctggccc acgatgtctg gattaccggt
     8821 aaacaggcag agaacaacgt taccgcagag attggttatg gtcataattt cccctcaaag
     8881 ggcacaattc ctgacagaag gaatttcttt gaaaatcccc ggctttataa cgggaaagag
     8941 ataataacgc tgaagccaac gtccacggat tatgtctata aaactgagtc tgcagccaac
     9001 ggtaatagct acgttctgtc aacgaaaatg aaaccgggat actggtcgag aacctcgtca
     9061 ggatggaagc ctgtcagtcg ggagggcaga aatgatgtgg catactgtga gtttgtgact
     9121 aaatatgcaa aatcttttat tcctggtgaa cagcagatgc cagcacaact ctatcagtct
     9181 ccaacagggg atgagcttga aatcattcca ttatccgata tcagtcgttt cggtgaagat
     9241 gtgaagctga aagttctgta taaaacatcc ccgcttgccg gagctactct ggagcttgac
     9301 tctgtcagtt atctgaaatc atcccgtcat acccatgcag ctgagcataa acattcggct
     9361 cataaagcag aacttacctt tgtaagtaat gaggacggta tcatcactgt accttccctt
     9421 catgtcgggc aatggctggc taaagtcaaa aataagaaag tatttccgga caagagtctg
     9481 tgtgatgaaa ctgtcgatgt ggcaacctta agcttctccc gaaattaata atactattcc
     9541 tgccctgatt tatgggcagg gtattatcaa tacgagaata gcctaccgac atccgtgtaa
     9601 aaaaccacat aatgattttt catatccatt tccagatata cttatgtaaa gatcacctcc
     9661 aaaaaagcgt aatagtaata aaaatccctg aagcaccctg ttcaaaccag aaggaataca
     9721 taacaagaca ataaaatgtg aaatccatca cagcaaagaa ctctttgttt ataatataa
//
LOCUS       DAZGXZ010000172         9585 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000172 DAZGXZ010000000
VERSION     DAZGXZ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.172"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(103..315)
                     /gene="cspA"
                     /locus_tag="ACHKJF_004619"
     CDS             complement(103..315)
                     /gene="cspA"
                     /locus_tag="ACHKJF_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP0951856.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(596..886)
                     /locus_tag="ACHKJF_004620"
     CDS             complement(596..886)
                     /locus_tag="ACHKJF_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP0951857.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1320..2030
                     /locus_tag="ACHKJF_004621"
     CDS             1320..2030
                     /locus_tag="ACHKJF_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP0951858.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2080..3054)
                     /gene="ghrB"
                     /locus_tag="ACHKJF_004622"
     CDS             complement(2080..3054)
                     /gene="ghrB"
                     /locus_tag="ACHKJF_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP0951859.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3158..3817)
                     /locus_tag="ACHKJF_004623"
     CDS             complement(3158..3817)
                     /locus_tag="ACHKJF_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP0951860.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3970..6303
                     /gene="bisC"
                     /locus_tag="ACHKJF_004624"
     CDS             3970..6303
                     /gene="bisC"
                     /locus_tag="ACHKJF_004624"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP0951861.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGKTVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6272..6712)
                     /locus_tag="ACHKJF_004625"
     CDS             complement(6272..6712)
                     /locus_tag="ACHKJF_004625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0951862.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6709..7272)
                     /gene="tag"
                     /locus_tag="ACHKJF_004626"
     CDS             complement(6709..7272)
                     /gene="tag"
                     /locus_tag="ACHKJF_004626"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP0951863.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7430..8128
                     /locus_tag="ACHKJF_004627"
     CDS             7430..8128
                     /locus_tag="ACHKJF_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0951864.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8357..9559
                     /locus_tag="ACHKJF_004628"
     CDS             8357..9559
                     /locus_tag="ACHKJF_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0951865.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSARLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2264 a         2353 c         2483 g         2485 t
ORIGIN      
        1 actagatttt attacgcgat cgattattta tttcctgaaa aacaaataaa aaaatccccg
       61 ccaaatggca gggatcttag attctgtgct tttaagcaga gattacaggc tggttacgtt
      121 accagctgcc gggcctttag cgccgctttc gatggtgaag gacactttct gaccttcgtc
      181 cagagatttg taaccatcgt tctggatagc agagaagtgt acgaacacgt ctttagagcc
      241 atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg aaccatttta cgataccagt
      301 cattttaccg gacatagtgt attaccttta ataattaagt gtgcctttcg gcgatatggc
      361 gtgctttaca gattttgaag cgttaaagga atgtgcacta cgaggggtat caacgataac
      421 tcttgaaggg acttgcctta ctacactgct ttaatggtct gtacgtcaaa ccgttgatgt
      481 gcattaagcc acgcattggc gggtgatgca acaattattt ttcatattta tgattaatcg
      541 gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat aaagtggata aaagtctatt
      601 ccatcaactg cttacttaat gccgggttgg cttgaatcaa acgcatcaat tttagttcgg
      661 cacttgaagg cttcacgcgt ctggattccc attcctttac catggcgact gatacgccca
      721 aaacccgggc gaaatcatcg atttttaacc ctgtcccttt tcgtaactgc tcaatttcgg
      781 tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt ttcatcttta aaaacaatct
      841 gttccaggct gctcaacagc tcatgcattg gatctttata ttccattgaa aactcctcaa
      901 atcacactgc gggatcgtga attgcatcga agctcattaa gaatagtcgg gaaaagcaga
      961 cacagcgggg tgaagtcagg gcatgctctg gctatgattc gattcagcca gtcatttagc
     1021 gtgacttata gccacctaac gatctgataa atgccgatat atctcttacc actcctggtt
     1081 tgtaaagatt tcgaaaatct aaccttctgc gaaggcgtaa atctctggct acaaaaaaag
     1141 caacggcact atcatttttt aacaatgaaa caacaaatta accttgtttc attttctcca
     1201 tgattttacc aggaagatta cgtagaaagg gtatcctgca agcctgtcct ggactatcct
     1261 tgtcacgtca gacacgcgtg tgtcgttgtg cgcttttttt gggtgaaagg agtaagaaaa
     1321 tggcgacagg aaagtcctgc tctcgctggt ttgcgcctct tgcggcgtta ttaatggtag
     1381 ttagcctgag tgggtgtttt gataaagaag gcgatcagcg taaagcgttt atcgacttcc
     1441 tgcagaatac agtgatgcgt agcggtgaac gtctaccaac cctgactgcc gatcagaaaa
     1501 aacagtttgg tccttttgtc tctgattacg cgattctgta tggttattct cagcaggtga
     1561 atcaggcgat ggattccggt ctgcgcccgg tcgtagacag cgttaacgca attcgcgtac
     1621 cgcaggatta cgttacgcaa agcggtccgc tgcgtgaaat gaacggttcg ctgggtgtgc
     1681 tggcgcaaca actgcaaaat gcgaagttgc aggccgatgc tgcgcactct gcgctaaaac
     1741 agaccgatga tttgaaaccg gtctttgatc aggcattcac caaagtggta acaacgccag
     1801 ctgatgcatt gcaaccgttg attccggctg cacaaacttt cacgcaacaa ctggtgatgg
     1861 ttggagacta cattgctcaa caaggtactc aggtgagctt tgtagcaaat ggtatccagt
     1921 tcccgacttc gcagcaggca agtgaatata acaaactgat tgcgccgtta ccggcacaac
     1981 atcaggcgtt taatcaggcc tggactacag cagttactgc aacccaataa agagtaaaag
     2041 cccgagcgat tatcgggctt tacttcacgc agtcgcggct tagtccgcga cgtgcggatt
     2101 cacacagttc ttctcaacct ttccttgtaa cgcatcaatc aaattatcca cggcacaggc
     2161 ggccatgcca taacgcgtct catgggtggc agatccaata tgcggtactg cgacgacgtt
     2221 ggccattgag agcaacggcg aatctacgga cagtggctct tgttcgaaga catccagccc
     2281 ggcggcgtga atttcgcctt tctgcaatgc agcaatcagt gcattttcat caaccaccgg
     2341 gccacgtccg gcattaatga aaatggcgga ggatttcatt ttggcgaatt gttctgcgcc
     2401 aaacagatga tgcgtctcat cagttaacgg caggatcagg caaacgaaat ctgactcttg
     2461 taacagagta tccaaatcgc agtagcgggc gttgaagcgt tcttctgctt ctttatggtg
     2521 gcggcgcgcg ttatagagga tgggcatgtt gaagccaaag tgcgcacgtt gtgccagcgc
     2581 catgccgatc cgtcccatcc cgacaatgcc cagtgtttta tggtgaacgt cagtgccgta
     2641 ccagtccggg cctatgctcg cggtccattc gcctgctttt acccgttctg ctacctccac
     2701 aacccgacga gcggtagaca acaccagcgc catcagcgta tcggcgacgg tttctgttaa
     2761 tacggttggc gtgtgcatca gcagaatttt tcgggcggta agcgcatcga catcaaaatt
     2821 gtcatagccg acggagatcg ttgatgtggc acgcagtttc ggcatttttt ccagcaatgc
     2881 ggcatctaca ttctcgtttg aacccagtaa accttcagct tcggcaaaaa ttgctgcatt
     2941 ttgttcgacg gtttgtgggc tgaggtttgc cacctggtga acggtgaaat gctcttgcag
     3001 gcgttgcagt aaatcatcag gtaaggcttt gtagaggata acggacggct tcatgcttct
     3061 ctccattcac tgataaggcg atcaccttag cctgaaatat gatgaaggaa aactgaagaa
     3121 tgccaggtga tttctgcatc acctggcatg aaagggatta cagcgggctt aaggtaattt
     3181 ctacacggcg gttttgcgcc ttaccttctg cggtgctgtt gctggcgatt gggttagccg
     3241 ggccaaggcc ctgagtacgg atgcggctgg cgtccacgcc ctgggtgatc aacgcgctgg
     3301 caacggaatc cgcacgttgc tgggagagac gcatgttcag gtcgtgacca cccgtgctgt
     3361 cggtataacc aatcacgtta accgccgttt tcggatactc tttcagcacc attgccacgc
     3421 cggtcagggt gttagcgcct gccggtttca gggtcgcgct gctgctgtcg aaggtcacat
     3481 tgttcggcat attgaggata atgttatccc cgctgcgggt tacgctaaca ccagtgccgc
     3541 gcattttgtc acgcagcttc gcttcctgca catccatgta ataacccacg ccgccgccca
     3601 gagctgcgcc tgctgctgcg ccaatcagcg cgcctttacc gcgatctttc ttcgaagaag
     3661 agagcgcacc aatacccgcg cccacgagag agcccagacc tgcgccgata gcagatttgc
     3721 ctgcttcgcg ttcgccggtg taagggttag ttgtgcagcc agataccgcc agagcgccac
     3781 tcactacggc ggcaataaga taaacacgtt tcttcattgt taatccttaa taaccttttt
     3841 attctttgcc acgggttccg tggcgggaga ttatgccgcg tgaacatgaa gattattcct
     3901 gggaatactc ggaaatttgt aagtaatatt taactgctca atacatctaa cctttcagga
     3961 gtcttcggtt tggccaactc atcctcacga tattccgttc tgactgccgc ccactggggg
     4021 cccatgctgg ttgaaaccga cggcaaaacc gtgtttagct cgcgtggcgc gttagccaca
     4081 ggaatggaaa actccttgca gagcgcggtt cgcgaccagg ttcacagcaa tacgcgggta
     4141 cgatttccaa tggtgcgcaa aggctttctc gcgtcaccgg aaaacccgca aggcattcgt
     4201 gggcaggatg aatttgttcg cgtgagttgg gatgaggcgc tggatcttat tcaccaacaa
     4261 cataaacgca ttcgtgaggc ttatggtccg gcatcgattt ttgctggttc ctacggctgg
     4321 cgttcaaacg gcgtgctgca taaggcctcg acattattac aacgctatat ggcgctggca
     4381 ggcggttata tcgggcatct gggggattat tcgaccggtg cggcacaggc gatcatgccg
     4441 tatgtcgtgg gtggcagcga agtttatcaa cagcaaacca gttggccgct ggtgctggaa
     4501 catagcgatg tcgtggtgtt gtggagtgct aacccactca atacgctgaa aattgcgtgg
     4561 aatgcatctg atgagcaggg gctttcttac ttttctgcac tgcgtgacag tgggaaaaag
     4621 ctgatctgca ttgatccaat gcgatcggaa accgtcgatt tctttggcga taaaatggag
     4681 tgggtggcac cgcacatggg caccgatgtt gcgctgatgc tggggatcgc ccatacgctg
     4741 gtggaaaatg gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc
     4801 ttcgcctctt atttgctggg cgagagtgac ggaatagcaa aaaccgccga atgggcagca
     4861 gagatttgtg gtgttggcgc agcgaaaatc cgcgagctgg cggctatttt ccaccaaaat
     4921 accaccatgc tgatggcagg ctggggaatg cagcgccaac agtttggtga gcaaaaacac
     4981 tggatgatcg tcacgctggc agcaatgttg gggcaaatcg gcacacccgg cggcggtttt
     5041 ggtctttctt accattttgc caatggtggt aaccccacgc ggcgttctgc ggtgctctct
     5101 tccatgcagg gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc
     5161 attgttgaag cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat
     5221 ttcccggata ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat
     5281 accaatcgtc tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc
     5341 tggacggcgg cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt
     5401 aatgatctca ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg
     5461 gtgccgccac gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt aagtgaacgc
     5521 tgggagaagg gcggttatgc acgttttacg gaaggaaaaa gtgagctgca atggctggaa
     5581 acgttttata acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt
     5641 gctgagttct ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg
     5701 tttattcgct tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc
     5761 ggcaagattg aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg
     5821 catccaatgt ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag
     5881 gtactttctg cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc
     5941 gaattgtacg cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg
     6001 cgcggcattc aagatggcga tactgttcgg ctgtggaacg cacgcgggca aattcttgcc
     6061 ggagcggtca ttagcgaggg aattaaacct ggcgtgattt gtattcacga aggggcatgg
     6121 ccggatctgg atttaaccgc tgacggtatt tgtaaaaacg gcgcagtcaa cgtgctgacc
     6181 aaagatctcc ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg
     6241 ctggaaaaat acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca
     6301 taatccaggt gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct
     6361 gcgcacggta aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg
     6421 gatagcgctg ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg
     6481 gtgcgacaaa cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct
     6541 taccgtcctc ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg
     6601 gaatgcagtc acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca
     6661 gccacagttc gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt
     6721 tcccggatag caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa
     6781 ggagtaacag attgtggtgc cgacaaactt aaaaccacgc tttttcaggg ctttagatag
     6841 agcgtcggag gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg
     6901 tggctgatga tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat
     6961 ttgcaggtac gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat
     7021 cccggcgtcc tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg
     7081 atcgaactga tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga
     7141 taacccagcc tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc
     7201 aggcacgccc cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc
     7261 gcaacgttcc atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct
     7321 tgacgcatcg gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat
     7381 acaatgacaa taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa
     7441 aaaaagcggt ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt
     7501 tctcaacaca gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc
     7561 cacagagcgt tacacctggg atagtgatca tcaacctgat tacaacgata ttttgtcgca
     7621 acgtattcaa agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc
     7681 tgtggatgtg accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac
     7741 aaccggcccg gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt
     7801 tggcgacagt actaccacgc tgaccgatcc gttatggcat gccagcgtga gtagcttagg
     7861 ctggcgtgtt gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca
     7921 gcaatttggc gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa
     7981 ccagaacggc aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat
     8041 tgccgcctat gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta
     8101 tacgatgggg gttagcgcca gattttaacg taacagtcac aattgaaacc attgaataac
     8161 aacagttgtg gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc
     8221 gcgtcattca ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt
     8281 ttccccaggc ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta
     8341 ctgcaggaat actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat
     8401 cggtacaatc attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg
     8461 cgcgctttcc gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt
     8521 aagtctgggg ctggcaattt catcttctgt tgcgggcaaa ttacaggaac gttttggcgt
     8581 taaacgcgtc accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca
     8641 ttctgacaac ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg
     8701 cgcgggttac ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct
     8761 gatatccgcg ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga
     8821 cacgcagctg ctggaaacgg tgggtctgga aaaaaccttt gtgatttggg gagcgattgc
     8881 gctggtgatg attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa
     8941 aaccagcaat ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca
     9001 gtactggatg ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg
     9061 ggtagcgaaa gatatcgccc aaagtctggc acaccttgat gtggtttccg cagctaatgc
     9121 tgtcactgtt atttccatcg ccaacctttc agctcgtctg gttctgggga tactgtctga
     9181 caaaatcgcc cgtatccgtg ttatcaccat tggtcaggtg atttcgctag tgggtatggc
     9241 ggccctgctg tttgcaccac tgaatgcagt gacgttcttt gcagcgattg cctgcgtggc
     9301 atttaacttt ggcggcacta ttaccgtctt tccgtcactg gtcagtgagt tcttcggcct
     9361 caataacctg gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt
     9421 tggttcgatt atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc
     9481 cctgctgatt ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt
     9541 gcgtgaggcg catatttaga aatatgttgc tactgaaata aatac
//
LOCUS       DAZGXZ010000173         9573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000173 DAZGXZ010000000
VERSION     DAZGXZ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.173"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..204
                     /locus_tag="ACHKJF_004629"
     CDS             <1..204
                     /locus_tag="ACHKJF_004629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951866.1"
                     /translation="LHFIPEMFAMKSAFRFFNSCYSRFMSTLDDASSSGCQYLLVEEI
                     KTKMAAGCYPCGRFRAHIAITAA"
     gene            complement(219..326)
                     /locus_tag="ACHKJF_004630"
     CDS             complement(219..326)
                     /locus_tag="ACHKJF_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0951867.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(731..1831)
                     /gene="chaA"
                     /locus_tag="ACHKJF_004631"
     CDS             complement(731..1831)
                     /gene="chaA"
                     /locus_tag="ACHKJF_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP0951868.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            2101..2331
                     /gene="chaB"
                     /locus_tag="ACHKJF_004632"
     CDS             2101..2331
                     /gene="chaB"
                     /locus_tag="ACHKJF_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP0951869.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2489..3184
                     /gene="chaC"
                     /locus_tag="ACHKJF_004633"
     CDS             2489..3184
                     /gene="chaC"
                     /locus_tag="ACHKJF_004633"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP0951870.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(3228..3581)
                     /gene="ychN"
                     /locus_tag="ACHKJF_004634"
     CDS             complement(3228..3581)
                     /gene="ychN"
                     /locus_tag="ACHKJF_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP0951871.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3766..5160
                     /gene="ychO"
                     /locus_tag="ACHKJF_004635"
                     /gene_synonym="iatA"
     CDS             3766..5160
                     /gene="ychO"
                     /locus_tag="ACHKJF_004635"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP0951872.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(5161..5811)
                     /gene="narL"
                     /locus_tag="ACHKJF_004636"
     CDS             complement(5161..5811)
                     /gene="narL"
                     /locus_tag="ACHKJF_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP0951873.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5804..7600)
                     /gene="narX"
                     /locus_tag="ACHKJF_004637"
     CDS             complement(5804..7600)
                     /gene="narX"
                     /locus_tag="ACHKJF_004637"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP0951874.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7939..9330
                     /gene="narK"
                     /locus_tag="ACHKJF_004638"
     CDS             7939..9330
                     /gene="narK"
                     /locus_tag="ACHKJF_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP0951875.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTHVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2324 a         2337 c         2447 g         2465 t
ORIGIN      
        1 cttcacttca tccctgaaat gtttgcaatg aagagtgcat tccggttttt caacagctgt
       61 tacagtcgtt tcatgagtac tctggatgat gcttccagct cgggttgcca atatttactt
      121 gtggaagaga taaagacaaa aatggccgca ggctgttacc cctgcggccg gtttcgggcg
      181 catattgcca tcacggcagc ctgacgcccg ttttcacctt acttccggtt acgccaccag
      241 ctgacaatcg ctgcggtaat aatccccgcc aggatcggtg ctgccaggtc gtgccagaaa
      301 gtcatggcaa actgcgcgag cgtcatatag ccgccttgtt gtaatgacaa cattttgcgg
      361 ctattcttga agtgtctggt ttcaagatta gcccccgttt tgttgtcagg ttttacctct
      421 caacgtgcgg gggttttctc tttccagcaa ccaatgccac cagggataaa gcccccgcaa
      481 cattgcgcct caccggataa ttccggcttg gtgtggatac tacgtctcaa ttcatcttca
      541 cttcatccct gaaatgtttg caataaagag tacattccgg cttttcaaca gctgttgcag
      601 tggtttcgtg caggttgtgg tacaggctcg caattctgat taacgagctg tccaggtagt
      661 atgaagaaat acaataagca ggaatttatc ttatctcccc cgatttccgt catcggagga
      721 gatataactg tcaggcaaat atcgtcatca aataggcggc aaacagtgcc agatgcgctg
      781 cgccattgag cacgttagta cgtccggtgg agaaggagat atggcacagc actaaagagg
      841 ccaccatcac caccatttct ggcgcaccaa gtgcaaactg taattcgtta cccgtcataa
      901 aggcaattag cgtgacgaca ggtacggtaa gcgaaatggt tgctaacact gaaccaaaga
      961 acagattcat cgcgcgctgg acctggttgt tcaacactgc ttttaacgca cctaaacctt
     1021 ccggcgacag aatcaacaga gccaccagga agccagtaaa ggcgacaggg gcattcatgc
     1081 tgtcgagcaa tgtctccagc gggctggcat tcattttggt gactgcaata acggcaatca
     1141 gatggataat caaccagata gcatgccaca cgctgctatg ggcagacggt ttaccgtgat
     1201 gcgggtcgtc gtcatcactg tcatcttcgt gctcgtagac aaacaaactt tgatgcgttt
     1261 tggtctggat cagcaaaaat acgccataca ttgccgcaga aattaatgct accagtaacg
     1321 cctgaccggt tgaaaaattc gccgcaggca gagccattgg aaataccagt acgattatcg
     1381 ccagggggaa cagcgcaatt aaatactgct tgataccaaa cagattcata tattgggtgg
     1441 caaacttacg accgcccaac aataatgaaa agccaaccag cccaccggta acaatcataa
     1501 tgattgaata gagcgtatca cgcattagcg ttggcgcggc gtcgccggtt gccattaaag
     1561 ctgaaatcaa actgacttca agaataacca ctgaaaggct aagaataagc gaaccgtaag
     1621 gttctcccag gcgatgggct aatacgtccg catgacggac aacactaaag gcgctactta
     1681 aaataccaat aagcgcaaga agattgatgg caatgaccac tggtagtgtc tggctgcttc
     1741 cccacaggaa cagcactacc agcgccagaa ccgggaaaat aagcgaagtc tccttgtggc
     1801 gggtttttac cgcctcttga gcatttgaca ttatggttat ccctttgcag atgaatttat
     1861 cgaaaatgta aaaaataggt agggaaaata acagaaagtg tctggatatc ggtaacattt
     1921 tacgaatttt taccccgctg tcgatttttt actcattggg gcataaaaat aagtattacg
     1981 tttagacaat gtttgtttta gcgtttctta agaagagtct gacatgaaaa ttcttatgtt
     2041 ttggcaagag tagatattgt tgaccacact taatgttcaa ctttgtaaaa ggagtcaacg
     2101 atgccgtata aaacgaaaag cgatctgccg gaaagcgtaa agcacgttct accgtctcat
     2161 gcccaggata tctataaaga agcgttcaac agcgcatggg atcaatataa agataaagac
     2221 gatcggcgtg atgacgccag tcgcgaagaa accgcgcata aagtggcctg ggctgctgtg
     2281 aagcatgaat atgccaaagg ggatgatgat aaatggcata aaaaatcgta aaaccggtag
     2341 cttagttaaa gctattcgtg cggtgttgcc ttgcaagtgg tccgtggatt gcatattgtc
     2401 ccgttagtgg tttcaaaatg agcagtaaaa atgtccggaa gacaccaaaa agttgtcgca
     2461 gggaagtatg cagtggcgga ggtgtaaggt gataacgcgt gatttcttga tgaatgccga
     2521 ttgtaaaacg gcatttggtg ccattgaaga atcactctta tggtcagcag aacaacgggc
     2581 ggcttcgctg gcggcgacgc tggcttgtcg acctgatgag gaaccggtgt ggatcttcgg
     2641 ctatggatcg ttgatgtgga atccggcact ggagtttacc gaatcgtgca ccggtacact
     2701 ggttggatgg catcgcgcat tttgcctgcg cctgaccgcc gggcgcggga ctgcgcacca
     2761 gccgggacgg atgcttgcac tgaaagaggg cggacgcacc acaggcgtcg cctatcgact
     2821 gccagaagag acgctggagc aggaactaac cttgttgtgg aagcgagaga tgattaccgg
     2881 ctgttatctg ccaacctggt gtcagcttga tcttgatgat ggacgcacag taaacgccat
     2941 tgtgtttatt atggatccgc gacatccaga atatgaatct gatactcgcg ctcaggtcat
     3001 cgcgccgttg attgcggcgg cgagcggtcc gctgggaacc aacgcacaat acctgttttc
     3061 actggaacag gagcttatca aactgggaat gcaggacgat gggctgaatg atttgctggt
     3121 atcggtaaaa aaactgctgg cggagaattt tccggatggt gtgttacgtc cgggattcgc
     3181 ctgaataaac ttcccgcata gtggggcgtc agacgccccc tcaaacatta aaatgtgagc
     3241 actttatcgg ctgacagcgt ccattgcgcc agttccacaa gagtaccgat ttccacccca
     3301 tcaatcagag gaagtgtact aatcccgcgc ccgtcggtac aggttttgca caatttcacc
     3361 ggtacattct gagcggtaag gatctccagc atttgctgaa tgttgtagcc ttcccctggt
     3421 ttttgcccgc gcaacccggc tgtgaccgca tcagacatga ggaacagacg cagatccaga
     3481 ttgctctcct gctctcgtaa cgcaatggcc agccgcaagc tgttaaacaa ggattcgctc
     3541 ccgtaaggtg cgccattggc aacgatcacg attttttgca ttatttactc ctgtattcag
     3601 ggaattagac actcatcttc tatcttactg cttctgcagc gtctgaccaa tcggtcacat
     3661 ttttaaggat tttcctgaaa gcgcgagaaa atacgacaaa agttgccagt aatcgttatt
     3721 ctttaaggct atggtttttc attattaccg gaagttaccg acgttttgag ccgtttcgtt
     3781 cctcgcatta tcccgtttta tttactcttg cttgtagcag gcggtacagc taacgcacaa
     3841 tctaccttcg agcaaaaagc ggcaaatccc tttgataata acaatgatgg tctgccggat
     3901 ttaggcatgg ctcccgaaaa tcatgatggg gaaaaacact ttgctgaaat tgtgaaagat
     3961 ttcggcgaaa ccagtatgaa tgataacggg ctggatactg gcgagcaggc aaaagctttc
     4021 gcattgggaa aagtccgcga cgcgcttagt caacaggtta atcagcacgt agagtcctgg
     4081 ctatcaccgt ggggaaatgc cagtgttgac gtcaaagtgg ataacgaagg acatttcacc
     4141 ggcagtcgtg gaagctggtt tgtgccgtta caagataatg atcgttatct cacctggagc
     4201 cagcttggtc ttactcagca ggatgatggg ttggtgagca atgtgggcgt tgggcaacgc
     4261 tgggcgcgcg gcaactggct ggtgggttat aacacttttt atgacaactt gctggacgaa
     4321 aatcttcagc gagcgggctt tggtgccgaa gcgtggggcg aatatttgcg actatcggca
     4381 aacttttatc agccgtttgc tgcatggcat gaacagacag ccacgcagga acaacggatg
     4441 gcgcgcgggt atgacctgac agcccggatg cgcatgccgt tctatcaaca cctcaatacc
     4501 agtgtcagcg tagaacagta ttttggtgat cgtgttgatt tgtttaactc tggtacgggt
     4561 tatcacaatc ccgtcgcgtt gagtctggga ttaaattaca cccctgtgcc attagtcact
     4621 gtgacggccc agcataaaca gggtgaaagt ggcgagaatc aaaataacct cgggctgaat
     4681 cttaattacc gctttggtgt accgctcaaa aaacaacttt ctgcgggtga ggttgccgaa
     4741 agtcagtcgt tacgtggtag tcgctatgac aatccgcagc gaaataatct accgactctt
     4801 gagtaccgac agcgaaaaac gttaacggtg tttctggcga caccgccgtg ggatctaaaa
     4861 cctggcgaaa cagtgccgct gaaattacaa atccgcagtc gttacggtat tcggcaactg
     4921 atttggcagg gcgatacgca gatattaagt ttgacgccgg gcgcacaagc caacagcgcg
     4981 gagggctgga cgctgatcat gcctgactgg cagaacgggg aaagggcgag caatcactgg
     5041 cgattgtctg tggtggtgga agataaccag gggcagcgtg tctcctccaa tgagatcacg
     5101 ctaacgcttg tcgaaccgtt cgatgcattg tcaaacgacg aactgcgctg ggaaccgtaa
     5161 tcagaaaatg cgctcctgat gcacccatac cgctgcttcc acgcgagact tgagcttcat
     5221 tttcttcagc atgtgcttga cgtgcacttt tactgtgctt tcggtgatat ccaggcggcg
     5281 ggcaatcatc ttgttcggca aaccctgggc aatcagcttg agaatatcgc gctcgcgtgg
     5341 ggttaactgg ttaacatcgc gctcagtagt ggcacggtta gcgcgcaagc tggcggccag
     5401 aacaggcgtt aatgcttcgc ttaataccat ttcgccagca gctgcctgat gcaatgcttt
     5461 cagcagatct tccggttcca tatcttttaa cagataacca tccgcgccgc gtttcagtgc
     5521 ggtgaccaca tcttcttcat ggttagagac gctgaatacc acaatgcgcc ctgagaggga
     5581 cttttcgcgc agtttatcca gcgtttccag accgttcatg ccgggcatat tgagatctaa
     5641 caggatcaga tcgggatcaa gagactccgc cagttcaata ccctgttcgc cattactcgc
     5701 ttcgccaacc acggtgatat ctggtgccat actgataagc tgttttacgc cagttcgcag
     5761 catcgggtga tcgtcgatca gcaggatagt agccggttcc tgattactca tgggtatctc
     5821 cttggacgtc tgtgaaagtt ttttcgggaa taaaggtgac caccacttcg gtgccacctg
     5881 attcacgacg gcggacgcgg caatcgcctc gtaaactttg cgcacggtcg cgcattatta
     5941 tcatgccgta gtgattgctg cggatggcat tttcaggcac gccgcagccg ttatcctgga
     6001 cggtcagttt gacctgatta tcgttttgcg ccaccgtcac cacgacttca ctcgcttgcg
     6061 aatgtttgag ggcgttactt aatgcctcac gggcaatttg caacaagtgg attgcctgat
     6121 gcgaaggcac caggcgaggc ggcaattgat aatccagctt caccgggaag ccaaatttgg
     6181 cgctgtactc ttcgcaactc gcctccagcg ccggacgtaa tccaggctcg gtgagctgca
     6241 agcggaatgt ggtgagcaat tcacgcaact gcgcccagga tgcattcagt tcgttacgga
     6301 tctgacttaa cagttcgcgg ctgctttctg gcagcgcatc gccctgcatc tgtaaacaac
     6361 tcacctgcat cttcatgcaa gagagagatt gggcaataga atcatgcagt tcgcgcgcaa
     6421 tggtggcacg ctcttccatc acgatcaact gttgctgacg ttcctgatgg cgatccagcg
     6481 ccagcgtggc ggtgagttgt tcaaccaggg tatccaccag ttgttgttga tcatggctaa
     6541 gatgacgccc ctgcggcagg gtcgccagca aaataccgta ctgcgtatga gagtcagcca
     6601 gccgccactt cagggttgtg ccgcgatcac caacgggtaa tacgccgcgc gggcagagct
     6661 ggcagccttt atcatcacaa gtcatatctg gttggcaggt aaactcctga tgattctctt
     6721 catcatcagt atcataaacc cgcagttcga tatcacgtag cagggttaaa ttctgtaagc
     6781 cgttgagtac aggtgacagg cgttcacaca gcggggcttg ggaatgcaaa cggcggttag
     6841 cctgccataa aaaagagagg atctgatttt tatgctccag cccggcggtt ttctcctgaa
     6901 cccgctgctc aagtacggca taactttcgg ccagttctgc agacatattg ttcaacgcag
     6961 ttccaagcat cgccatttcg ttgcgcccgc tgatgtttgc gcgttgggta aaatcgcgat
     7021 gactgacggc actcgccatt gccagcagtt gccgccacgg ttgtagcagt cgcgcccgca
     7081 accagataat agtgaacacc agtaaaagtg ccataaatac cgccattacc cgatggacca
     7141 gtaccactgt ctcgatgcgc atttccgtgg tgcggtcaaa accagatacc agttgatcaa
     7201 gcccggcaac aaactggctg acatccgctg acaccgtttc tcggttttgt gcacgcatca
     7261 acgcagggat cagttcatta cgccagtaat cttgtaaacc ctgtaattgc gccagttgtc
     7321 cgtctcgttc tgctgctcga gtcaactcgg cgctaaatgc cgtttgttcc atctctttaa
     7381 ttaagggctt gtctttctcg cttaatggca ctgctgccaa cagacggtaa ctttgcatgc
     7441 gcagcgatcc cgctttgttg atcgcatggg cgctgccctg aacgccttgc accagccagc
     7501 cagaaaccgc catccctgcc agtccaatag cagtagaaag caacacaata agcgcaacct
     7561 gattaaccag ggtgagcgga gagagacaac gtttaagcat gtaaacctct tccttcaggc
     7621 tttaaatgag caataacctt aatgaatgtg acgatacatt ctggaatggc agtattctcg
     7681 gctattggct gaagtatacc catacctgga aagagttact ccttatttgc cgtgtggtta
     7741 gtcgctttac atcggtaagg gtagggattc tacagcaccg tgaaaaatct cataattttt
     7801 atgaaatcac tgtactcact atgggtaatg ataaatatca atgatagata aagttatctt
     7861 atcgtttgat ttacatcaaa ttgcctttag ctacagacac taaggtggca gacatcgaaa
     7921 cgagtatcag aggtgtctat gagtcactca tccgcccccg aaagggctac tggagctgtc
     7981 attacagatt ggcgaccgga agatcctgcg ttctggcaac aacgcggtca acgtattgcc
     8041 agccgcaacc tgtggatttc cgttccctgt ctgctgctgg cgttttgcgt atggatgttg
     8101 ttcagcgctg ttgcggtgaa cctaccgaaa gtcggcttta attttacgac cgatcagcta
     8161 tttatgttga ctgcgctgcc ttcggtttct ggcgcgttat tacgtgttcc atactccttt
     8221 atggttccta tcttcggtgg tcgtcgctgg acggcgttca gcaccggtat tctgattatt
     8281 ccttgcgtct ggctgggttt tgccgtgcag gatacctcca cgccttatag cgtcttcatc
     8341 atcatctctc tgctatgcgg ctttgctggc gcgaacttcg cctccagtat ggcaaacatc
     8401 agcttcttct ttccgaaaca gaagcagggt ggcgcgctgg gtctgaatgg tggtctgggc
     8461 aacatgggcg tcagcgtcat gcagttggtt gctccgctgg tggtatcact gtcgattttc
     8521 gcagtatttg gtagccaggg cgtcaaacag ccggatggga ctgagctgta tctggcgaat
     8581 gcgtcctgga tatgggtgcc gttccttgcc atcttcacta ttgcggcgtg gtttggcatg
     8641 aacgatcttg ctacctcgaa agcctccatc aaggagcagt tgccggtact caaacggggt
     8701 catctgtgga ttatgagcct gctgtatctg gcaaccttcg gctccttcat cggcttctcc
     8761 gcgggctttg caatgctgtc aaaaacgcag ttcccggatg ttcagattct gcaatacgct
     8821 ttcttcgggc cgtttattgg tgcgctggcg cgttctgcag gtggtgcatt atctgaccgt
     8881 ctgggcggaa ctcatgtcac gctggtgaac tttattctga tggcgatttt cagcggcctg
     8941 ctgttcctga ccttaccgac tgacgggcag ggcggaagct tcatggcgtt cttcgcggtc
     9001 ttcctggcgc tgttcctgac agctgggctg ggtagtggtt ccactttcca gatgatttca
     9061 gtgatcttcc gtaaactgac aatggatcgc gtgaaagcag aagggggttc tgacgaacgt
     9121 gcgatgcgtg aagcggcaac cgacacggca gcggcgctgg gtttcatctc tgcgattggc
     9181 gctattggtg gcttctttat cccgaaagcg tttggtagct cgctggcatt aacgggttcg
     9241 ccagtcggcg caatgaaggt atttttgatt ttctatatcg cctgcgtagt gattacctgg
     9301 gcggtatatg gtcggcattc taaaaaataa accgttactc gtcatacttc gggttacatg
     9361 tgctgcggct gcgttcattc accccagtca cttactttag taagctcctg ggattcattc
     9421 acttgccgcc ttcctgtaaa ccgaattata tagagtaaaa tatttgatta tcctttgcgc
     9481 ggcatgatgt cgcgcttttt ttatgcgtta tttagttaca acatactaat gttatatggc
     9541 ttattcgccg gatttcatta agagccatta ata
//
LOCUS       DAZGXZ010000174         9554 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000174 DAZGXZ010000000
VERSION     DAZGXZ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.174"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..370
                     /locus_tag="ACHKJF_004639"
     CDS             <1..370
                     /locus_tag="ACHKJF_004639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951876.1"
                     /translation="RKNILRHSVRNQMAEIKGKITNPALVNAKVFYNRRTKLGIPTEV
                     FLILMFLNVFAVVLCVMYFSTFSVISLILFVLVSVVPMKFVFHGDPDAHKAWIYGYLS
                     PSRLNNTHAVKRPITFIDAI"
     gene            400..747
                     /locus_tag="ACHKJF_004640"
     CDS             400..747
                     /locus_tag="ACHKJF_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951877.1"
                     /translation="MLNRISRKISTFQIMLYVALVTGSVTPAMAAGNSTVKLPTPDDS
                     FLQKIVNWMQSILDLVGGAGALFFAFVSGFACIVLWGLAPQQSQAIGKLFRAAVAAIA
                     AFNLAFFIAWLQK"
     gene            759..3359
                     /locus_tag="ACHKJF_004641"
     CDS             759..3359
                     /locus_tag="ACHKJF_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4 family type IV secretion system protein"
                     /protein_id="HFP0951878.1"
                     /translation="MGQSYNEHLNLAGIEQHSGQVYTYTGGAFGAIELTGVDPTGVTP
                     SQGSRISLLLRNIVQLLPLSVTLTQYYIHNDGVKIRLTGRDNQRSQLLSMRRQAYLNK
                     CRNLNASRIIWTLDVHPEENINRLASAEFLKNLFNSMFDDTARRKVRLALSNRESFII
                     ERQAFEKQCLLLKETLRDLDLRLSFISEDNRILDSEGIWRLQKFLATFNHHWLAPRRK
                     VTVPNDMWDCLALEGDDIEAITWRGVDMLKIQGVRPVYVRLASVLKIGDESVPECAWA
                     LTNGRPVLQRGNYVYFMRFTPASAFKKSLMISGKENELVRTQISLSDLMTDSTSKDIL
                     DEKLRRNSHLMKMREELENISYSDERLGICQAGIAVFNTDPDKLLETCADINRSVSQT
                     MQVVWETVAQLDAYKVMQPAYSRNTFRALQLNASQMGAASLFFKSHPGIPEWELNGRM
                     EEAFYILESDDGVPFFFTPKVKEKLLLLGNGATRGGKTFMKNCIASHFIKFGGLYNCL
                     DIDPGSIPMANFFGDDGAAFTLDSDFQCGFNMFSMAQSKDDRVFLAHLIEQLKAMAAE
                     NDSEQDKYLTPDELENITVCASDLLVQKFSPNEGRLSSITLSTLVSKCGQSVKRKLAP
                     FISNGIYGRLFDNKEDAIGVLDKPVAVYNLSGVKDNHNLARLVHREIFFRVVRLFESP
                     EYRTIPKFFEIDEAQYTLSVPHAAEFATQKARTWFKHGGGMGFWTQNPEHYSSLDEWT
                     TLRSSASVFIFMPDNEATVKAFHDAYGFDEEEVNIIRSLKVKQQSYIKIPDMQIAKVV
                     NLFVEAEQYAVCTSTSNEAALAAEIWKRESDPDVAVRQIVEGLITKCNKDLIPTPTET
                     EKDKTEGFFL"
     gene            3356..4177
                     /locus_tag="ACHKJF_004642"
     CDS             3356..4177
                     /locus_tag="ACHKJF_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFP0951879.1"
                     /translation="MRKQLAVILFSVMTGLITTPAISSGIPVADAAQILGQARAMMQD
                     IKNYKEYMEQTILNNKELLEAYKQYNQMLEDYKQILREAEGLKDKLSSIDYKNFMDEI
                     ALISNQFDPVYGGGRMVKNTGNAPWDNAYERNLLLNGYGLTNEEYAEMLAAIPYTSDD
                     RERARQMFEYRQRRVVEGISKDAFVGELQIQLKKQGKTLGELQERRLRLGDNDTTASI
                     QFLAEQNEQLLQQIQSLQLQQAEAMKYSGRLEDHYFNKRAQQEELKARQLQRAWQ"
     gene            4174..4641
                     /locus_tag="ACHKJF_004643"
     CDS             4174..4641
                     /locus_tag="ACHKJF_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951880.1"
                     /translation="MKKYLILFFMMFSASAMAKIGYVDEHQKEVDLKVDALISKYEKE
                     CEGKRNSNMCKSQAWDKAHFEYEDEFRGEDKYNHKHYDGLTKDQAVAKLHELIKLHNI
                     VSKDERNPESWPGKLDTLTINGEINYIVRKHWPAWINPCDKICAELLLRQIGK"
     gene            4701..5672
                     /locus_tag="ACHKJF_004644"
     CDS             4701..5672
                     /locus_tag="ACHKJF_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951881.1"
                     /translation="MSLINRLLFVVLFLSVSFYASAETAMRTNFQNGVSEMVNKILNS
                     PDSDLRVWTIAASTFLAAVTAFVYFWRFVENGGALVDLFTLAIMFMLSMSLLASYSMV
                     VDTITGAFYAISNTLQKLALGNIKPSFLQNFVEGVLTKAISAPSVDIFDGMNMVGMAL
                     LWEICSAILSVAIYLADVWIDVGTAIAKITGVMFIPFLVAPATRGIFDGWFKFYIGWG
                     LAAVVLKITSIVVMIMIRASINSAGSFQGAGDVIASDGTVNGVLNITANNISLLRDIC
                     AYSVIAAIMVFSCFIFAYMIGSGVGSASQGLNNMAVKLAKKAAKALL"
     gene            5682..6401
                     /locus_tag="ACHKJF_004645"
     CDS             5682..6401
                     /locus_tag="ACHKJF_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VirB8/TrbF family protein"
                     /protein_id="HFP0951882.1"
                     /translation="MLLEKKEIEKVNRTSFKDRVGELKKDRNLFFWIALLLAGTTIYQ
                     SYSVQEAIEKSQNTTEVVWVKLLPDGSHKISQFTPDNEQPIYTPTVNSLLSKYVQKRY
                     GQLKETIERDYAEAAVFMSPVLTGEFADPKGFNVAQKVVDIQADMAAKRIYIEIKDID
                     HYDAIEGQFDGDVKPVIRTLVTYQEITRDYQGKEISSVQQMRRLQWTLLQRNELSKQS
                     LDWLNINPLGIQIIDDKSVQQ"
     gene            6414..7247
                     /locus_tag="ACHKJF_004646"
     CDS             6414..7247
                     /locus_tag="ACHKJF_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbG/VirB9 family P-type conjugative transfer
                     protein"
                     /protein_id="HFP0951883.1"
                     /translation="MLAKRTLLFSGVLAVVMTTFSATAQQCRVVNWRPGTIVKVQSAL
                     YLGTRIELPEGSMLLTKEPFSSNALWASTGAANQVMIQPTSEEPEGLKTMVTVFMTNG
                     QTLDIEAVRVSQNQNQQCVQIRGAGMRSADRSALNTAYSMGASGADVARLQNEVNTLR
                     SNRESEVKNAVLEALRRYRYFIYTRYNWTTGKGFSGKNMISDVYDDGRFTYIRIAKPN
                     RGLLAVHADVGGKPAVIPTKYDDAYAIYHMSGIYPKFTLTLDGVKLEVSRADNATDGE
                     S"
     gene            7250..8656
                     /locus_tag="ACHKJF_004647"
     CDS             7250..8656
                     /locus_tag="ACHKJF_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbI/VirB10 family protein"
                     /protein_id="HFP0951884.1"
                     /translation="MEERQEKVEEKPATYRKKIQYYAFGTFGVILIILFINMVISIAS
                     RNEEEPKENAKQETASTAPNTTEKFNALLKNSGARPSASVNQANTVGNKDSASNGNPN
                     SETDKKPVHPYSREKEASQERGARASDAGSPDEIRARFKADEINRALRARSDKTNFDS
                     TKNTVAGSGNAYAPAASFTGNRTNAQRISDINAEQNGLQERIQMMESKVRADEAALPG
                     RLEEARIRGEQQFAEARQGGAGSNATSVTKGSTQSVPSGVVGYTASNPYGASVDGMMK
                     LPVGTVLNAITTFTAISDYSGGSMKAMLTHDIYDATHSYILAPKGSEFVIRVVKASQV
                     NEILQSRIGFQVTWLVLPDGNRVDFKTSSSLDRMGTPAVEGDEVDRHLLAQFLGVAAY
                     ALVGTKTSYEGSGDSNESFAGNFGEGARTQAGNIAQKYLQVVPTTTLHAGAPIRVFTE
                     DEIYMKPWRNIYETDYTG"
     gene            8727..9128
                     /locus_tag="ACHKJF_004648"
     CDS             8727..9128
                     /locus_tag="ACHKJF_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951885.1"
                     /translation="MRESAPVQIPQQDVHVQGNFQELQPLDRNQKDPRWQYIDPLSVY
                     MRTVWPDNIRTIRAAVNYLLEPTGYRLVTSHPAPREASLLVDKPLPPIARMQRTMPVV
                     DALQLLVGLDNYVVIDRAHRLVSFQKNGGGV"
     gene            9140..9349
                     /locus_tag="ACHKJF_004649"
     CDS             9140..9349
                     /locus_tag="ACHKJF_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951886.1"
                     /translation="MRIIFVAFLSIATVILCLFMTGCAQRYESGHDQLFELSERHIIY
                     RDLTVPASMSNLPEKQQISVPGIEP"
     gene            9367..>9554
                     /locus_tag="ACHKJF_004650"
     CDS             9367..>9554
                     /locus_tag="ACHKJF_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0951887.1"
                     /translation="MLAFLISGGISASTDEEILLASITAPGVTSFTDESPSSEWITAM
                     QGRWGSTTDMKDMRSLRE"
BASE COUNT         2744 a         1771 c         2359 g         2680 t
ORIGIN      
        1 acgtaaaaat atactacgtc attcggtaag aaatcaaatg gctgaaataa aaggaaaaat
       61 aaccaatccc gcactggtta atgcaaaagt tttttacaac agacgaacaa agctggggat
      121 acctacagag gtctttttga tcctgatgtt cctgaatgtt tttgctgttg tgctgtgtgt
      181 tatgtatttc agcacatttt ccgttatttc gctgatttta tttgttctgg tatcggttgt
      241 tcctatgaag ttcgtttttc atggcgatcc cgatgctcat aaagcttgga tttatggtta
      301 tttgtcacct tccaggctga acaataccca cgcagtgaag cgtcctatca catttataga
      361 cgctatttag ggatttaacc agtaaaagag gtgtatacaa tgctcaatcg catttccaga
      421 aagatatcaa cattccagat aatgctttac gtagcattag tgacaggaag tgtcacaccg
      481 gctatggctg ctggtaatag tacagttaaa cttcctacgc cggatgatag ttttttacag
      541 aaaatcgtta actggatgca aagtattctg gatctggttg gtggggctgg agctttgttc
      601 tttgcgtttg tcagtgggtt tgcttgtatt gtgttgtggg gacttgcacc gcaacagagc
      661 caggctatcg ggaaactgtt tcgtgctgct gttgcagcaa ttgcagcgtt taacctggcg
      721 ttctttattg cctggctgca aaaatgaggt gatccacgat ggggcagagc tataacgaac
      781 atctgaatct ggcaggcatc gaacaacatt ccgggcaggt gtatacctat actggaggtg
      841 cttttggtgc tattgaactg acaggtgttg atccaacggg tgttacccct tctcagggta
      901 gccgtatttc tttattactt cgtaatatcg ttcagttact gcccctttct gtcacactga
      961 ctcagtatta tatccataat gacggcgtta agatccggct gacagggcgt gataatcaac
     1021 gcagtcagtt actttctatg cgtcgccagg catatcttaa taaatgtcgt aatttaaatg
     1081 cctcaagaat tatatggacg ctggatgttc acccggaaga gaatattaat cgcctggcat
     1141 ctgccgagtt tttaaagaat ctttttaaca gcatgtttga tgacacagca agacgtaaag
     1201 tcaggctggc actgtcaaat cgcgaatcgt ttatcattga gcgtcaggca tttgaaaaac
     1261 agtgcctgtt gctgaaagaa acattacgcg atctggactt acgcctttct tttatttcag
     1321 aagataacag gattcttgat tcagaaggga tctggcgatt acagaagttc cttgcaacat
     1381 ttaatcacca ttggctggct ccacgccgta aggtcactgt acccaatgat atgtgggatt
     1441 gtctggcgct tgaaggtgac gatatcgagg ccattacctg gcgtggcgtt gatatgttga
     1501 agatccaggg tgtacggcct gtttatgttc gcctggccag tgtgttgaaa ataggcgatg
     1561 aatctgtacc tgaatgtgcc tgggcgctga caaatggcag accagtttta cagcggggta
     1621 actacgttta cttcatgcgg ttcactcccg ccagtgcctt taagaagtca ctgatgatca
     1681 gcgggaaaga aaacgaactt gtcaggactc aaatcagttt gagcgattta atgactgaca
     1741 gtacgagcaa agatattctc gatgaaaaat tgcgcagaaa ttcacatctg atgaagatgc
     1801 gcgaagaact ggagaatatt tcatattcag atgagcgcct ggggatctgc caggccggga
     1861 ttgctgtatt taatacagat cccgataaat tacttgagac ttgtgcggat attaaccgta
     1921 gtgtctctca gaccatgcaa gttgtatggg agacggtagc acaactggat gcttacaagg
     1981 ttatgcaacc agcctactca cgcaatacat ttcgtgcatt acagcttaac gcatctcaga
     2041 tgggggctgc atccttgttc tttaaatccc atccaggtat tcctgaatgg gagctaaatg
     2101 gacgcatgga ggaagccttc tatattcttg aatcagatga tggcgtgccg tttttcttta
     2161 cgccaaaagt taaagaaaag ctactgcttc ttggtaatgg ggcgacccgt ggcggcaaga
     2221 cttttatgaa aaactgtatt gccagccatt ttattaaatt cggtgggctg tataactgtc
     2281 tggatatcga tcctggctca atcccaatgg ctaatttctt tggggatgat ggggcagcat
     2341 ttacccttga tagcgatttt cagtgtggtt ttaacatgtt cagtatggcg caaagcaagg
     2401 atgacagggt atttcttgcg catcttatag agcagttaaa agcaatggca gcagaaaatg
     2461 acagtgagca ggataaatat ttaacgcctg atgaacttga aaatataaca gtgtgtgcga
     2521 gtgatcttct tgtacaaaaa ttttcgccga atgaggggcg tttaagctca atcacgttgt
     2581 caacgctggt ttctaagtgt ggtcagagcg ttaaacgcaa gttagcgcca tttattagta
     2641 atggtattta tggccgttta tttgataaca aagaggacgc gattggtgtt cttgataagc
     2701 cggtagctgt ttacaacctt tcaggtgtga aagataacca taatcttgcg cggcttgtgc
     2761 accgtgaaat cttctttcgt gtcgttcgtt tgtttgaatc accggaatat cgcacgatac
     2821 caaagttttt tgaaatagat gaggcacagt acaccctgtc tgtaccacat gccgcagagt
     2881 tcgctacaca gaaagcccga acctggttta agcatggtgg gggtatggga ttctggactc
     2941 agaacccgga gcactattca tcactggatg agtggactac gttacgttcc tcggcgtcgg
     3001 tgtttatctt tatgccggat aatgaggcaa cagttaaagc attccatgat gcttatggct
     3061 tcgatgaaga agaagtgaat attattcgca gccttaaggt aaaacagcag tcgtatataa
     3121 agatcccgga catgcagatt gcaaaagtgg tgaatctgtt tgttgaggct gaacagtatg
     3181 ctgtttgtac atcaacgtct aatgaagcag ccctggcagc ggaaatatgg aaacgtgaat
     3241 cagatcccga tgttgcagtg cgacagattg tagaaggtct gataacgaag tgtaataagg
     3301 acttgatccc aacgcctaca gagactgaaa aggataaaac ggaggggttt ttcttatgag
     3361 aaaacaactt gcagtaatct tgttttctgt aatgacagga ttgattacaa cacctgctat
     3421 ttcttctggc attcctgttg ctgatgctgc tcagattctt ggtcaggcca gggctatgat
     3481 gcaggatatc aaaaactata aagaatatat ggaacagaca attctcaata ataaagaatt
     3541 gctggaagca tataagcaat acaaccagat gcttgaggat tataagcaaa ttttacgtga
     3601 ggccgaaggg ctgaaagata aactttcatc gattgactat aagaatttta tggatgaaat
     3661 agcccttatc tctaaccagt tcgatcccgt ttatggcggg gggcgtatgg ttaaaaatac
     3721 gggtaatgcg ccgtgggata atgcctatga gcgtaatttg ttgcttaatg gctatgggct
     3781 tacaaacgaa gaatatgccg aaatgctggc agcgatacca tatacgagtg atgaccgcga
     3841 acgtgcccgg cagatgtttg aatataggca gcgcagggtt gttgaaggga tttcaaaaga
     3901 tgcctttgtt ggtgaattgc aaatacaatt aaaaaagcaa ggaaaaactc taggggaatt
     3961 acaggaaaga cgtttaaggt taggtgataa cgatacgaca gcctctatcc agtttttagc
     4021 tgaacaaaat gaacaactgt tacaacaaat tcagagttta cagttacagc aagcagaggc
     4081 gatgaaatat agtggtcgtc ttgaggatca ctattttaac aagcgagcgc aacaggaaga
     4141 attaaaggca cgtcagttac agagggcatg gcaatgaaaa aatatctgat tcttttcttc
     4201 atgatgtttt cagcctcggc aatggctaag attggctatg ttgatgagca tcaaaaggaa
     4261 gttgacctaa aagttgacgc tctgatttct aaatatgaaa aagaatgtga aggtaaacgc
     4321 aatagtaata tgtgtaaatc ccaagcatgg gataaggctc attttgaata tgaagatgaa
     4381 tttagaggcg aagataaata taatcataag cattatgatg gcctgacaaa agatcaggca
     4441 gtcgcgaagt tacatgaact tattaaattg cataatattg tttctaaaga tgaacgtaat
     4501 cccgaaagtt ggcctggaaa attagatacg ctaactatta atggtgagat taactatata
     4561 gttagaaaac actggccagc atggattaat ccatgtgata agatttgtgc ggaactgtta
     4621 cttagacaaa taggtaaatg atgtagcatt taacaagcta tatctttaat ttgggggcta
     4681 ttatgcttcg aggattcttt ttgtctttaa ttaacagact cttatttgta gttctgtttt
     4741 tatctgtctc attttatgcc agtgcagaaa cagctatgcg cacaaacttt cagaatggcg
     4801 taagtgagat ggttaataaa atactaaata gcccggatag tgatttgcga gtctggacta
     4861 ttgccgcatc aactttcctg gctgcggtaa cggcattcgt ctatttctgg cggttcgtgg
     4921 aaaatggtgg ggcgttagtt gatttattca cgcttgcaat catgtttatg ttatcaatgt
     4981 cattgcttgc ttcttactca atggttgttg atactatcac aggcgctttt tatgcgatat
     5041 caaatacact acagaaactg gcgttaggta atattaagcc gagttttctg caaaattttg
     5101 tagagggcgt gttaacaaag gctatttcag caccatcggt tgatattttt gatggcatga
     5161 atatggttgg catggcgctc ttgtgggaaa tatgttctgc tattttgtct gttgctatat
     5221 atcttgctga tgtttggatt gatgttggga cagcaatagc aaaaattaca ggtgttatgt
     5281 ttattccttt tttggttgcg ccagcaacaa gaggaatttt tgatggatgg tttaaattct
     5341 atattggatg ggggcttgca gcggttgtac ttaaaataac atctattgtg gtaatgataa
     5401 tgatcagggc atcaattaac tctgcgggta gtttccaggg cgctggcgat gttatcgcgt
     5461 cagatggtac tgttaatggc gttcttaata taacagctaa taatatcagc ttgttacgtg
     5521 atatttgtgc atattctgta atcgctgcaa ttatggtgtt cagttgtttt atatttgcgt
     5581 acatgatcgg ttctggtgtt ggttctgcaa gtcagggctt gaataatatg gcagtgaaac
     5641 tagccaagaa agcggctaaa gcattattgt aaggtgaacg tatgctgctt gaaaaaaaag
     5701 aaatcgaaaa agtaaatcga acatccttta aggatcgtgt tggtgaacta aaaaaagaca
     5761 gaaatttgtt tttctggatt gctttgcttc ttgccgggac gacgatatat cagtcatatt
     5821 ctgttcagga ggcaatagag aaaagccaga atactacaga agtagtatgg gtgaaattat
     5881 tgcctgatgg ttcacataaa atatcacaat ttacgccaga caatgagcag cctatatata
     5941 caccaacagt gaactcattg ctgtcaaagt atgtgcagaa gcgctatgga cagttaaaag
     6001 agactataga acgggattat gcagaagcgg cagtatttat gtctcctgta cttacagggg
     6061 agtttgccga tcccaaagga tttaatgtcg ctcagaaagt tgtggatatt caggccgata
     6121 tggcagcaaa aaggatttat atagaaataa aagatattga ccattatgac gcaatagaag
     6181 gtcagtttga tggtgatgtc aaacctgtaa ttcgaacgct tgttacttat caggagataa
     6241 caagggatta tcagggtaaa gaaatttcat cggttcaaca aatgaggcgt cttcaatgga
     6301 cgttgttaca acgtaacgaa cttagtaaac aatcgctgga ctggcttaac atcaatccgc
     6361 tgggaatcca gattattgat gataaatccg tacaacagta aaggaactta atcatgttag
     6421 cgaaaagaac tctcttattt tctggagtcc tggctgttgt catgactacg ttcagtgcaa
     6481 cagcacagca atgtagggtg gttaactggc ggcctggaac aatagttaaa gtgcagtccg
     6541 ctttatatct gggaacccgt atagaactgc cggaaggctc aatgttatta acgaaagaac
     6601 ccttttcttc taatgcgcta tgggccagca cgggagccgc aaatcaggtc atgattcagc
     6661 ctaccagtga agagccggaa gggctgaaaa cgatggtaac tgtatttatg actaacgggc
     6721 agacgctgga tattgaggcc gttcgggtat ctcagaacca gaatcaacag tgtgttcaga
     6781 tccgtggtgc aggtatgcgt tccgcagaca gaagtgcatt aaacacggct tacagtatgg
     6841 gggcatccgg ggctgatgta gctcgtttac agaatgaagt gaatacattg cggagtaaca
     6901 gagaaagcga agtcaaaaac gcagttcttg aagctcttcg tcgataccga tatttcattt
     6961 atacccgtta taactggacg acaggtaaag gttttagcgg aaagaatatg atctctgatg
     7021 tttatgatga cgggcgtttt acttatatcc gtatcgcaaa accgaatcgt ggattacttg
     7081 cggtacatgc agatgtcgga ggaaaaccag cggttattcc taccaagtac gacgatgcat
     7141 atgccattta tcatatgtca gggatatatc ccaaatttac ccttaccctg gatggcgtaa
     7201 agctggaagt cagccgtgcg gataatgcga ctgatgggga gagctaatta tggaagaacg
     7261 tcaggagaaa gtagaagaaa aaccagctac ttaccgtaaa aaaatacagt attacgcttt
     7321 tggtactttt ggtgtgatcc tgataattct tttcatcaat atggtgataa gtattgcctc
     7381 aaggaacgag gaagaaccaa aggagaatgc gaaacaggaa actgccagca ctgcgccaaa
     7441 tacaacggaa aagtttaatg cgctactgaa aaacagtggt gcacgtcctt ctgcctccgt
     7501 taatcaggct aatacggttg gaaataagga tagtgccagt aatggaaatc cgaatagtga
     7561 aacagataaa aaacctgttc acccttacag ccgggaaaaa gaggcgtctc aggagagggg
     7621 ggcgagagca agtgatgcgg gatcacctga tgaaatcagg gcacgcttta aggctgatga
     7681 gattaaccga gcactgcgtg caaggtcaga taaaacaaat tttgacagca ctaaaaatac
     7741 ggttgcgggt agtggtaatg catatgcccc ggctgcatcg tttaccggaa accggactaa
     7801 tgcgcagcgt atcagcgata taaacgcaga gcagaatgga ttgcaggaac gtatccaaat
     7861 gatggaatca aaagttcgtg ctgatgaagc cgctttgcca ggacgactgg aagaagcccg
     7921 gatacgtggg gaacagcaat ttgcagaagc ccgacagggt ggagcaggaa gtaacgcgac
     7981 atcggtaact aaggggagta cccagagcgt accgtccggt gttgtgggat atacagccag
     8041 taatccttat ggggcaagtg ttgatggaat gatgaagttg ccagtaggta cggtcctgaa
     8101 tgccattact acatttacgg ctatttctga ttattccggc ggctcaatga aggccatgct
     8161 tacgcatgat atctatgatg cgacgcatag ctacattctt gcgcctaaag gaagtgagtt
     8221 cgttatcagg gtcgttaaag cctcccaggt aaacgaaatc cttcaaagcc gtattggatt
     8281 ccaggttaca tggcttgtat tgcctgatgg taacagggtc gattttaaaa cctcgtcgtc
     8341 tcttgaccga atggggactc cggcggttga aggtgatgag gtggacagac atttgctcgc
     8401 tcagtttctt ggcgtagcgg cctatgccct tgtggggacg aaaaccagct acgaaggctc
     8461 aggtgatagt aacgagagtt ttgccgggaa ttttggcgaa ggcgctcgta ctcaggcagg
     8521 aaatatcgct caaaaatacc tccaggttgt gcctacaaca acgttacatg cgggagcacc
     8581 gatcagggtc tttaccgaag atgagatata catgaagccg tggagaaata tttatgaaac
     8641 ggattatacg ggctgaaata ttgttttttg ctgcattctt tgcattttcc tgtcaggcag
     8701 caagaagccc tttccaggta gacaggatgc gagaaagtgc gcctgttcag ataccacagc
     8761 aggatgtgca tgtgcaggga aactttcagg agttacaacc actggacagg aaccagaaag
     8821 atcctcgctg gcagtatatc gatccgttga gtgtttatat gcgtacagtt tggcccgata
     8881 acatcaggac tatcagagca gcggtgaact atctcctgga accaactgga tatcgcctgg
     8941 taacgagtca tccggcaccg cgtgaggctt ctctgcttgt tgataagcca ttaccgccaa
     9001 ttgccagaat gcaacgcaca atgcccgtag ttgatgctct tcagttgctg gttgggcttg
     9061 ataattatgt tgttattgat cgcgcccaca ggctggtttc atttcagaaa aatgggggcg
     9121 gtgtatgaag gaatggtgta tgcggattat ttttgtggct ttcctgtcta ttgcaacggt
     9181 gattctttgc ttatttatga cggggtgtgc acaacgttat gagagtggtc atgatcagct
     9241 tttcgaactg tcagaacgcc atatcattta ccgggatctg actgttccgg catcgatgag
     9301 caatttgccg gagaagcagc agataagcgt accggggatt gagccgtgaa cgtccggtta
     9361 attttcatgc tggcgtttct gatatctggc ggtatcagcg catcaacgga tgaagaaatt
     9421 ctactggcgt cgataaccgc gccaggagtt accagcttta ccgatgaatc accttcttcg
     9481 gaatggatta cagcgatgca gggacgttgg ggtagtacaa ctgatatgaa ggatatgcgt
     9541 agtttgcgcg agat
//
LOCUS       DAZGXZ010000175         9430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000175 DAZGXZ010000000
VERSION     DAZGXZ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.175"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            14..577
                     /gene="fimA"
                     /locus_tag="ACHKJF_004651"
     CDS             14..577
                     /gene="fimA"
                     /locus_tag="ACHKJF_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0951888.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            938..1648
                     /locus_tag="ACHKJF_004652"
     CDS             938..1648
                     /locus_tag="ACHKJF_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0951889.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            1690..4341
                     /locus_tag="ACHKJF_004653"
     CDS             1690..4341
                     /locus_tag="ACHKJF_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0951890.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSATNGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPGTLLNQQTAICR"
     gene            4355..4885
                     /gene="ydeS"
                     /locus_tag="ACHKJF_004654"
     CDS             4355..4885
                     /gene="ydeS"
                     /locus_tag="ACHKJF_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951891.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4898..5401
                     /gene="ydeR"
                     /locus_tag="ACHKJF_004655"
     CDS             4898..5401
                     /gene="ydeR"
                     /locus_tag="ACHKJF_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951892.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            5461..6375
                     /gene="ydeQ"
                     /locus_tag="ACHKJF_004656"
     CDS             5461..6375
                     /gene="ydeQ"
                     /locus_tag="ACHKJF_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0951893.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSARQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            6709..8988
                     /gene="ydeP"
                     /locus_tag="ACHKJF_004657"
     CDS             6709..8988
                     /gene="ydeP"
                     /locus_tag="ACHKJF_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP0951894.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            9236..>9430
                     /gene="safA"
                     /locus_tag="ACHKJF_004658"
     CDS             9236..>9430
                     /gene="safA"
                     /locus_tag="ACHKJF_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP0951895.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT         2657 a         2056 c         2152 g         2565 t
ORIGIN      
        1 attttggaga attatgaaac tcaaacatgt tggtatgatt gtcgtttctg tgttggcgat
       61 gtcgtctgct gcggtaagcg cagccgaggg tgatgaatca gtaacgacca ctgttaatgg
      121 cggtgttatt cattttaaag gtgaagtggt aaatgccgct tgtgcaattg attcagaatc
      181 aatgaaccaa acggttgagc tgggtcaggt tcgttcttct cgcctggcta aagcgggtga
      241 cctcagctcc gccgttggct tcaatatcaa gctgaatgat tgtgatacca atgtttccag
      301 taatgcagct gttgcattcc tgggtactac tgtcaccagt aatgacgata cgttagcgct
      361 gcagagttca gcggcaggct ctgcccaaaa tgtcggtatt caaattttgg accgtacggg
      421 tgaggtatta atacttgatg gggccacttt tagtgctaaa accgacttga ttgatggcac
      481 gaatatactg ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc
      541 aaacgctgat gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg
      601 tcatctgaaa ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caatttacca
      661 atattcatct catttttaat gagaaatggt ttgtatttgg agcgctatga aaaatcacat
      721 aatatctaaa ctatcaataa acaacagtag ttaaacttac tgttatttaa aatatttact
      781 ttgtagcaag gttttcctat taaaagagaa ccaacattta ttacaaacgc attaagtctg
      841 gaaacccggc gactcgccaa tgtattagca acattaatcg attgtctgat gtaacggttt
      901 tcccgcttat cctcttaaca catggagaat gcatcagatg caaactacgc gtacccccta
      961 ctctatttcc tttatggcga cagtgttact tttattgctc tttgcttgcc atagcaccgt
     1021 tgccaacgca gctgtcgcac tgggtgcgac gcgggtaatt tatccggcaa accagaaaca
     1081 ggttttattg cctgtcacga ataatgatcc tgccagtgtc tatctcattc agtcatggat
     1141 agaaaatgca ggtgaccaaa aagacaccca gtttgtcatt accccaccgc ttttttccat
     1201 gcaaggtaaa aaagagaata cgctgcggat aatcaacgct actaatcatc agttaccagg
     1261 cgaccgtgaa agcctgttct gggtgaatgt taaagctatt cctgctatgg aaaaagacca
     1321 gaagaatgag aatacattac agctggcaat tattagccgc ataaaaatgt tttatcgccc
     1381 cacgaacctg gcaatggcac ctgaagaagc gcctgcaatg cttcggtttc gtcgcagcgg
     1441 tagcaaactc acgcttatta atccaacgcc ctatttcatt accgtaacaa atatgaaagc
     1501 aggaaatagc aatctcccaa acaccatggt gccgccaaaa ggtgaagtat cggtggatat
     1561 cacgcatgct gcaaccgggg atatcagttt tcagacgatc aatgattacg gtgcattaac
     1621 tccgcgtatt aaggcaacca tgcagtaagc cagtgaccta attaataata aactcaatcg
     1681 gaattaataa tgacagccat tcgtgccgca tttaaggcat atcgtatgca tcaggtgtta
     1741 ataatgcctc gctttgcacg gcttaccatt gcccttggct tagcaactgc ggtatttccc
     1801 gttgacgcag agtattattt taatccacgg tttttaagca atgatcttgc tgaatctgtc
     1861 gatttatccg cctttactaa gggacgcgag gcaccacctg gtacatatcg ggtcgacatt
     1921 tatttgaacg atgaatttat gactagtcgg gatattactt ttattgcaga tgataataat
     1981 gcagaactca tcccatgcct gagtacagat cttcttgtca gtcttggtat aaaaaaatca
     2041 gcgttattag acaataaaga acattcggca gaaaaacatg tgccggacaa cagcgcatgc
     2101 acaccacttc aggatcgttt ggccgacgca tcaactgagt ttgatgttgg tcagcaacat
     2161 ttgtcactct ccgttccgca aatttatgtt ggcagaatgg ctcgcggcta tgtttctcct
     2221 gatctgtggg aagaaggaat aaatgctggg cttctaaact atagttttaa tggtaattct
     2281 attaataatc gtagtaacca taatgcagga aaatcaaact atgcatattt gaatttacag
     2341 agtggcatca acattggtag ttggcgacta cgcgataact caacgtggag ttataacagt
     2401 gggagtagca attcatctga cagcaataaa tggcagcata tcaatacgtc ggctgaacgt
     2461 gacattattc ccttacgctc acggttaacg gtaggtgata gttataccga tggcgatatt
     2521 tttgatagtg tgaactttcg tggcctcaaa ataaattcaa cagaagcgat gttgcccgat
     2581 agccaacatg gtttcgctcc tgtgattcat ggtattgccc gtggaaccgc acaagtgagt
     2641 gtaaaacaga acggatacga tgtttatcag actactgttc cacccggccc ttttactatt
     2701 gatgatatca actctgcgac caatggtggt gatctgcaag taaccataaa agaggcagac
     2761 ggcagtattc agacattata tgttccttat tcgtctgttc cggttctcca acgtgctgga
     2821 tatacgcgtt atgcgcttgc catgggggaa tatcgtagtg gaaataacct gcaaagctct
     2881 cccaagttca tacaaggtag cttgatgcat ggtctggaag gaaactggac accttatggc
     2941 ggaatgcaaa ttgcagaaga ttatcaggcc ttcaaccttg gtattgggaa agatttagga
     3001 ctttttggtg ccttttcttt cgatatcacg caggccaata cgacacttgc agatggcacc
     3061 cgtcacagtg ggcaatcggt taaatccgtc tacagcaaat ccttctacca gacaggaacc
     3121 aatatccagg tcgcaggata tcgctattct acgcaaggtt tttataactt atccgacagt
     3181 gcctacagtc gaatgagtgg ttacaccgtc aagcctccta ccggagacac caatgagcag
     3241 acacaattta ttgattattt taatctgttc tacagtaagc gtggtcagga acaaataagc
     3301 atctctcagc agcttggaaa ttacggtacg acatttttca gtgccagtcg ccaaagttac
     3361 tggaacacgt cacgcagcga ccagcaaata tcatttggat taaatgtgcc gtttggtgat
     3421 attacgactt cgctgaatta cagctattcc aataatatat ggcaaaacga tcgggatcat
     3481 ttactcgctt ttacgcttaa tgttcccttc agtcattgga tgcgtacaga cagtcagtcg
     3541 gcatttcgta attcaaacgc cagttacagt atgtcaaacg atttgaaagg cggcatgacc
     3601 aatctatcgg gggtttatgg cactctgctg ccggataata acctgaatta tagcgttcag
     3661 gtcggtaaca cccacggagg taatacatcg tctggcacca gtggttacag ttctcttaat
     3721 tatcgtggag cttacggtaa tacgaatgtc ggttacagtc ggaatggtga cagcagccag
     3781 atttattacg gaatgagtgg tgggattatt gctcatgctg atggcatcac ctttggacag
     3841 ccgctgggcg acacaatggt tctggttaag gctcctggcg ctgataatgt caaaatagag
     3901 aaccagaccg gaattcatac cgactggcgt ggctatgcaa tattaccatt tgcgacagaa
     3961 tatagagaaa atcgtgtcgc tcttaacgcg aattcccttg cagataatgt tgaactggat
     4021 gaaaccgtag tcactgtcat cccaactcac ggtgctattg ccagagcaac atttaatgca
     4081 caaatcggcg ggaaagtatt aatgacgttg aagtacggta ataaaagcgt tccattcggt
     4141 gcaattgtca ctcacggaga gaataaaaat ggcagcattg tcgcggaaaa tggtcaggtt
     4201 tatctgactg gacttccaca gtcagggaaa ttacaggttt catggggcaa tgataaaaac
     4261 tcaaactgta ttgtcgatta caagcttcct gaagtctctc ctggaacctt gctgaaccag
     4321 cagacagcaa tctgtcgcta aggaacaata gattatgaaa tacaataacg ttattttcct
     4381 cggtttatgt ctggggttaa ccacctattc tgctttatcc gcagatagcg ttattaaaat
     4441 tagcgggcgc gtcctcgatt atggctgcac agtctcatcg gattcgctta attttaccgt
     4501 agatctccaa aaaaacagtg ccagacaatt tccaacgacc ggtagcacaa gtccagccgt
     4561 cccttttcag attacgttaa gtgaatgcag caaagggaca acgggggttc gggttgcatt
     4621 taacggtatt gaggatgcag aaaataatac tctgttgaaa ctggatgagg gaagcaatac
     4681 ggcctccggt ttaggtatag aaatactgga cggaaatatg cgtccggtga aactgaatga
     4741 ccttcatgcc gggatgcagt ggatcccact ggtaccagaa cagaacaata ttttgcctta
     4801 ctccgctcgt ctgaagtcaa ctcagaagtc cgtcaatccg ggactggtga gggcttcggc
     4861 aacctttacc cttgaatttc aataatcaga gtcagatatg aaaagacttc acaagaggtt
     4921 cctgttagct acgttttgcg cgttattcac agcaactctc caggccgccg atgtcactat
     4981 cactgttaat ggtcgggtag tcgctaaacc ctgcactatt caaaccaaag aagctaacgt
     5041 taatctcggg gatctttata cgcgcaatct gcaacaacct ggttctgcat ctggctggca
     5101 caatattact ttgtcattaa ccgattgtcc ggttgaaaca agtgcagtga cggcaatcgt
     5161 gacaggttca actgacaata cgggttatta caaaaatgaa ggtactgccg aaaatattca
     5221 gatagagctg agggatgacc aggatgcgac gttaaaaaat ggcgatagca aaacggttat
     5281 tgttgatgag atcactcgta atgcacagtt tccacttaag gcaagagcta tcacggtgaa
     5341 tggaaacgca agccagggaa cgatcgaggc gctaatcaat gtgatctaca cctggcaata
     5401 acgtcgattc gattttctta gccttctttt taaatcaatt ttttaacctt aggatatatc
     5461 atgggtaaaa caatcagtat aaaggtcctg ttcggtattt atcttctatt aatggcaggt
     5521 aaagtttttg ccttcagttg taatgtcgat ggcgggtcaa gtattggagc tggtacaacc
     5581 tctgtttatg ttaatctcga ccctgtaata cagccgggcc agaatctggt tgtagacttg
     5641 tctcagcata taagttgctg gaatgattac ggcggctggt acgacactga tcatataaac
     5701 ctggtacaag gctcagcttt cgcaggctct ctacagtcat ataaaggatc gctgtactgg
     5761 aataatgtga cctacccctt cccgttgacg acaaatacca acgtattgga tattggtgat
     5821 aagacaccga tgccattacc gttaaaactc tatattaccc cagtcggggc ggccggtgga
     5881 gttgtcataa aagccgggga agttattgcc cgtattcata tgtataaaat tgcaacatta
     5941 ggaagcggaa atccgcgtaa ttttacgtgg aatattattt caaataacag tgttgttatg
     6001 ccaacaggcg gctgcactgt tgatagtcgt aatgtcaccg tggatctccc ggactttcct
     6061 ggaagcgcag aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat
     6121 ctttcgggtg ccaccactga cagtgcccga caagtgtttg ctaacacagc acctgatgca
     6181 acaaaagcat cgggagtggg tgtctctctg atgcgtaatg gaaaaatact ggctacggga
     6241 gaaaatgtct ccctgggaac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc
     6301 tatggacaaa caggaaacaa ggtttcagcc ggaaccgtcc agtcagtcat tggtgtcact
     6361 tttatctatg aataattgtg attttgatct tagtctatgc gctccattca cgaaaattgc
     6421 gcggataatt attttgtaaa ggctattatc ctacacctgt aagaaaatcc gcgcacaatt
     6481 gaagccagag tcttattttt tatattgaaa aatatgttaa tttaaacact atgagagatg
     6541 atgcttaact tatcgtattt aatctattgt ttaacgataa tagattaaaa atagaaagag
     6601 agtagtcccc tactcttata tatccatgtt ggcgataatc cctttgtata tactgtgcat
     6661 catcgctatt acaaatccta ataattcatt tccacacagg ataagtagat gaagaaaaaa
     6721 attgaatcct accagggtgc tgccggtggt tggggtgctg ttaaatccgt agcgaatgca
     6781 gtacgtaagc agatggatat acgccaggat gttattgcca tgtttgacat gaataagcca
     6841 gagggctttg actgtccggg ttgtgcatgg ccagatccta agcacagtgc gtcattcgac
     6901 atttgtgaaa acggcgcaaa agcaatcgcc tgggaagtca cggataagca ggtaaatgcc
     6961 tctttctttg ctgagaatac ggttcaatca ttacttacct ggggagatca cgagcttgag
     7021 gctgcgggac gtctcactca gcctttgaaa tatgatgctg tcagcgactg ttacaagcca
     7081 ttaagctggc aacaagcttt cgacgaaatt ggcgcacgcc ttcaaagcta tagtgatccc
     7141 aatcaggttg aattctatac ttcgggccgc acttccaatg aagctgcctt tctttatcag
     7201 ctttttgccc gtgaatacgg gagtaataac tttcccgact gctccaacat gtgccatgaa
     7261 ccgaccagtg tgggtttggc agcgagtatc ggtgtgggta aagggactgt gttgctggaa
     7321 gactttgaga agtgcgattt agtcatttgc attgggcata accctggtac aaaccacccc
     7381 cgcatgctga cttcgttgcg cgctttagtg aaacggggag cgaaaatgat cgccatcaat
     7441 cctctacagg aacgtggcct ggagcgattt accgcaccgc aaaacccgtt tgaaatgctg
     7501 acgaactctg agactcagtt ggctagtgcc tactataacg tgcgcattgg tggcgatatg
     7561 gcgttgctca aggggatgat gcgcctgtta attgagcgcg atgatgctgc aagtgccgca
     7621 ggtcggccct cattgctgga tgacgaattt attcaaacgc ataccgtcgg ctttgacgag
     7681 ctacgccgtg acgttctcaa ttccgagtgg aaagatatcg aacgtatttc tggattaagt
     7741 cagactcaaa tcgccgaact ggctgatgca tatgccgctg ccgaacgaac cattatctgt
     7801 tacggaatgg ggatcactca gcacgaacat ggtacccaga atgtacagca actggtcaat
     7861 ctgctgttga tgaaaggtaa cattggcaag cctggtgcgg gtatctgccc actacgtggc
     7921 cactctaatg tacagggcga ccgaaccgtc ggtatcaccg agaaaccgtc tgcagagttt
     7981 ctggctcgcc tgggtgagcg ctatggcttc acaccacctc aagcacctgg acatgctgca
     8041 attgccagca tgcaagcaat atgtacaggg caggctcgag cattgatctg catggggggc
     8101 aactttgcac tggcaatgcc agatcgggaa gcgagcgctg taccgttaac gcaattagat
     8161 ctggcggtac acgtagccac taagcttaac cgctctcatc tgctgaccgc acggcatagc
     8221 tatattctgc cggttctggg acgtagcgag attgacatgc aaaaaagcgg tgcgcaggcg
     8281 gtaaccgttg aggattcaat gtcgatgatt catgcctcgc gtggcgtgtt aaaacccgcc
     8341 ggtgtaatgc tgaaatcaga gtgtgcagtg gtcgcgggaa tcgcgcaggc agcactaccc
     8401 cagagcgtgg tagcctggga gtatctggtg gaagattatg atcgcattcg caatgacatt
     8461 gaagctgtgc tgccagagtt cgccgactat aaccaacgca tccgtcatcc cggtggtttt
     8521 cacctgataa atgcagctgc tgaaaggcgc tggatgacgc cgtcaggtaa ggctaatttc
     8581 attaccagca aagggctgtt agaagatccc tcttcagcgt ttaacagtaa gctggtcatg
     8641 gcgacagtac gcagccacga tcagtacaac acgacgattt atggtatgga tgatcgctat
     8701 cgaggggtat tcggtcaacg agatgtggtc tttatgagtg ctaaacaagc taaaatttgc
     8761 cgtgtaaaaa acggcgaaag agttaatctt attgcgctca cgccagacgg taaacgtagc
     8821 tcacgccgca tggatagatt aaaagtggtc atttacccta tggctgaccg ctcactggtg
     8881 acctattttc cagaatcgaa tcacatgcta acactcgata accacgatcc attaagtggc
     8941 attcctggct ataaaagtat tccggttgaa ttagaaccat caaattaatg cctcttctca
     9001 tttcttctgc tgtcatccgc acagcagaag aatccctcat tgactattat cgcgcaattt
     9061 gctcacatgg attaaattaa actacatact ataagatata aacctctgcc tacagctgta
     9121 agaaactccg ctcagtactg aagtgccagt cctatttcct cttttctcca gcctgttata
     9181 ttaagcatac tgattaacga tttttaacgt tatccgctaa ataaacatat ttgaaatgca
     9241 tgcgaccaca gtgaaaaaca aaatcacgca aagagacaac tataaagaaa tcatgtctgt
     9301 aattgtggtt gtcttattac tgacccttac gttgatagcc attttttcgg caattgatca
     9361 gctgggtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     9421 tttgcaagac
//
LOCUS       DAZGXZ010000176         9423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000176 DAZGXZ010000000
VERSION     DAZGXZ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.176"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            385..1256
                     /locus_tag="ACHKJF_004659"
                     /pseudo
     CDS             385..1256
                     /locus_tag="ACHKJF_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            1628..4474
                     /locus_tag="ACHKJF_004660"
     CDS             1628..4474
                     /locus_tag="ACHKJF_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFP0951896.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADTVVQAGETVNDGTLTNHDNQIVLGTANGMTISTGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQAIKSGAVATDTVVNTGAEGGPDAENGDTGQTVYGDAVRTTINK
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNDGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATNVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDILESHSAQNTLVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGISGQASGLLLENGGSFTVNAGGQAGNTTVGYRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIHFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     VHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     QYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMNFGKGTSSRDTLRDSAK
                     HSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSDSSAEGYNGQATLNVTF"
     gene            4545..4778
                     /locus_tag="ACHKJF_004661"
     CDS             4545..4778
                     /locus_tag="ACHKJF_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0951897.1"
                     /translation="MPGCTSRLLPEGPFSRNQALAVTTAYLNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            4869..5687
                     /locus_tag="ACHKJF_004662"
     CDS             4869..5687
                     /locus_tag="ACHKJF_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0951898.1"
                     /translation="MRLASRFGYVNQIRRERPLTREELMYHVPSIFAEDRHTSRSERY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRREYTKHTLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPALQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSARGKRIHTRAIHSIDTDIKLNRALWVMAETMLESMR"
     gene            5777..6262
                     /locus_tag="ACHKJF_004663"
     CDS             5777..6262
                     /locus_tag="ACHKJF_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0951899.1"
                     /translation="MTTVSHNFTTPSVSVTTASGNNQSQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCENYCDGIWNLYTLNNGGAFMAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            6278..6754
                     /locus_tag="ACHKJF_004664"
     CDS             6278..6754
                     /locus_tag="ACHKJF_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFP0951900.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            6817..7038
                     /locus_tag="ACHKJF_004665"
     CDS             6817..7038
                     /locus_tag="ACHKJF_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0951901.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWQGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            7201..7575
                     /locus_tag="ACHKJF_004666"
     CDS             7201..7575
                     /locus_tag="ACHKJF_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0951902.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVKPCFGARLVQEGNRLHYL
                     ADRAGISGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHSVTLYAKGLTCEAD
                     TLGSCGYVYLTVYPTPATATTS"
     gene            7622..7999
                     /locus_tag="ACHKJF_004667"
     CDS             7622..7999
                     /locus_tag="ACHKJF_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0951903.1"
                     /translation="MKTLSDTHVREASRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMTRDNYRMVNNITQGKHPEAQQ"
     gene            7996..8484
                     /locus_tag="ACHKJF_004668"
     CDS             7996..8484
                     /locus_tag="ACHKJF_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0951904.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGIELAELINKVAENGYS
                     LHVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            8496..8693
                     /locus_tag="ACHKJF_004669"
     CDS             8496..8693
                     /locus_tag="ACHKJF_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0951905.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            8778..>9423
                     /locus_tag="ACHKJF_004670"
     CDS             8778..>9423
                     /locus_tag="ACHKJF_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0951906.1"
                     /translation="MSGITISRPEVVNGHTDVICSTSIGHILAVRKTTLMQIDTLIRQ
                     LTEISAMTESIGGKTAPDWAMKQNFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISAANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIP"
BASE COUNT         2230 a         2593 c         2544 g         2056 t
ORIGIN      
        1 tcaatgctac tcctatcgtt cttttgcgtc acagagcgcc tgccggacag ctgcatcata
       61 gaccaaacgg aactgcttcc gtcgattaat cagatggcag cgcataaaca cgctcaacaa
      121 cccgtttgac tgcttcaatt ttaagctctt cggaataacg cttaccgtgt aagtctccct
      181 gcaaaacgag ctatcaactt ttaaggagtc tctggccccc ctgcttccgc tcctggcaca
      241 gagcggactg tcagattaga ttcagacctg tatcagccac atatacctgc attccaaata
      301 atttcagaca gatattatcg ttgtgaatcc cccgccacct ttccggtgcg cggggttttg
      361 tcttttttca ccgggaatac atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa
      421 ccgctctcct ccctgcctta ctcactttcc cgtcacatcc tggaacacct gcgcaaactc
      481 accagtcacg aacccgtgat tggcattatg ggtaaaagcg gggccggtaa atcctcactc
      541 tgtaatgcac tgtttcaggg ggaggtcacc ccggtcagtg atgttcacgc cggcacccgg
      601 gaagtgcggc gcttccgtct gagtggccac ggtcacagca tggttatcac tgactgcccg
      661 gggtgggcga gagccgggac agggatgcag agtatgaagc cctgtaccgt gacattctgc
      721 ccgaactgga cctggtgctg tggctgatta aagccgatga ccgtgccatg tctgtggatg
      781 agtatttctg gcgacacatc ctgcaccagg gacatcagca ggtgctgttt gtggtgacac
      841 aggccgacaa aacggagccc tgccatgaat gggatatggc cggtattcag ccttctcccg
      901 cacaggcaca gaatattcgc gaaaaaacgg aggcggtatt ccgtctgttc cggcccgtac
      961 atccggttgt ggccgtatcg gcccgcaccg gctgggaact ggatacgctg gtcagtgcgc
     1021 tcatgacagc gcttcccgac catgccgcca gtcccctgat gacccgcctg caggacgagc
     1081 tgcgcacgga atctgtccgc tctcaggccc gtgaacagtt taccggtgcg gtggaccgga
     1141 tatttgacac ggcggagagc gtctgtgttg cctctgttgc ccgtacggtt ctgcgtgccg
     1201 tccgtgacac ggtggtctct gttgcccgcg ctgtatggaa ctggattttc ttctgaacac
     1261 tctgccagga aaatgcctga ttacattgcg ttaaccaact gaaaacatga cacaaacacc
     1321 tcatgctcct cctgtttctc ttgcgtgacc gccttgcaat caatagaata aaacgatcga
     1381 taaaacagat atcgatcgtt actatcgatc gtttatagtg gttaatggtc tgcgaaaaag
     1441 cagttgaatc tcttcatcat gcagaacgga attgcacaaa acggactgat acttctctgg
     1501 cggatgagag aggggaagca tttatgtccg ggaaaaacca ctgtatctga ccatgctgtt
     1561 ttgtacctgc cggtatccac atttgtgggt accggctttt ttattcaccc tctgtaagga
     1621 aaagctgatg aaacgacatc tgaacaccag ctacaggctg gtatggaatc acattacggg
     1681 caccctggtg gtggcctccg aactggcccg ctcacgggga aaacgcgccg gtgtggcggt
     1741 tgcactgtct cttgctgctg tcacatcagt cccggcactg gctgctgaca cggttgtaca
     1801 ggcgggagaa accgtgaacg acggaacact gacaaatcat gacaaccaga ttgtcctcgg
     1861 tacggccaac ggaatgacca tcagtaccgg gctggagtat gggccggata acgaggccaa
     1921 taccggcgga caatggatac aaaatggcgg tatcgccaac aacactactg tcaccggtgg
     1981 tggtcttcag agagtgaatg ccggaggaag cgtttcagac acggttatca gtgccggagg
     2041 cggacagagc cttcaggggc aggcagtgaa caccacgctg aacggcggtg agcagtgggt
     2101 acatgaaggc gggattgcaa cgggtaccgt cattaatgag aagggctggc aggccatcaa
     2161 atccggcgca gtggcaaccg acacggttgt gaataccggc gcggaagggg gaccggatgc
     2221 ggaaaatggt gataccgggc agaccgtcta cggagatgcc gtacgcacca ccatcaataa
     2281 aaatggtcgt cagattgtgg ctgctgaagg aacggcaaat accactgtgg tttatgccgg
     2341 cggcgaccag actgtacatg gtcacgcact ggataccacg ctgaatgggg ggtaccagta
     2401 tgtgcacaac gacggtacgg cgtctggcac tgttgtaaac agtgacggct ggcagattat
     2461 caaggaaggt ggtctggcgg atttcaccac cgttaaccag aaaggcaaac tgcaggtgaa
     2521 cgccggtggt acagccacga atgtcaccct gaagcaggga ggcgcactgg tcaccagtac
     2581 tgcagcaact gttcttggca gcaaccgtct ggggaatttc acggtggaaa acggtaaggc
     2641 tgacggtgtt gttctggaat ccggcggtcg tctggatata ctggagagcc attcagcaca
     2701 gaataccctg gtggatgacg gcggtaccct ggcagtgtct gccggcggta aggcgacagg
     2761 tgtcaccatg acatccggtg gtgccctgat tgcagacagt ggtgccactg ttgaggggac
     2821 caatgccagc ggtaagttca gtattgatgg tatctccggt caggccagcg gcctgctgct
     2881 ggaaaatggc ggcagcttta cggttaatgc cgggggacag gccggtaaca ccactgtcgg
     2941 atatcgtgga acactgacgc tggctgccgg gggaagtctg agtggcagaa cacagctcag
     3001 taaaggcgcc agcatggtac tgaatggtga tgtggtcagt accggcgata ttgttaacgc
     3061 aggagagatt cactttgata atcagacgac accggatgcc gcgctgagcc gtgctgttgc
     3121 aaaaggcgac tccccggtaa cgttccataa actgaccacc agtaacctca ccggtcaggg
     3181 gggcaccatc aatatgcgtg ttcgccttga tggcagcaat gcctctgacc agctggtgat
     3241 taatggtggc caggcaaccg gcaaaacctg gcttgcgttc acaaatgtcg gaaacagtaa
     3301 cctcggggtg gcaaccaccg gacagggtat ccgggttgtg gatgcacaga atggtgccac
     3361 cacagaagaa ggtgcgtttg ccctgagccg cccgcttcag gccggcgcct ttaactacac
     3421 cctgaaccgt gacagcgatg aagactggta cctgcgcagt gaaaatgctt atcgtgctga
     3481 agtccccctg tatgcctcca tgctgacaca ggcaatggac tatgaccgga ttctggctgg
     3541 ctcccgcagc catcagaccg gtgtaaacgg tgaaaataac agcgtccgtc tcagcattca
     3601 gggcggtcat ctcgggcacg ataacaacgg cggtattgcc cgtggggcca cgccggaaag
     3661 cagcggcagc tatggcttcg tccgtctgga gggtgacctg ctcagaacag aggttgccgg
     3721 tatgtctctg acgacagggg tgtatggtgc tgcaggccat tcttccgttg atgttaagga
     3781 tgatgacggc tcccgcgccg gcacggtccg ggatgatgcc ggcagtctgg gcggatacct
     3841 gaatctggta cacacatcct ccggcctgtg ggctgacatt gtggcacagg gaacccgcca
     3901 cagcatgaaa gcatcatcgg acaataacga cttccgcgcc cggggctggg gctggctggg
     3961 ctcactggaa accggtctgc ccttcagtat cactgacaat ctgatgctgg agccacaact
     4021 gcagtacacc tggcaggggc tctccctgga tgacggccag gataacgccg gttatgtgaa
     4081 gttcgggcat ggcagtgcac aacatgtgcg tgccggcttc cgtctgggca gccacaacga
     4141 tatgaacttt ggtaaaggca cctcatcccg tgacaccctg cgcgacagtg caaaacacag
     4201 cgtgagtgaa ttgccggtga actggtgggt acagccttct gttatccgca ccttcagctc
     4261 ccggggtgac atgagcatgg gtacagccgc agccggcagt aacatgacgt tctcaccgtc
     4321 ccagaatggc acgtcactgg acctgcaggc cggactggaa gcccgtgtcc gggaaaatat
     4381 caccctgggc gttcaggccg gttatgccca cagcgtcagc gacagcagcg ctgaaggtta
     4441 taacggtcag gccacgctga atgtgacttt ctgataatca ggtcactccc cttcaggccc
     4501 cggtcatcat gaccggggcc ttttacttac agggagcaac agatatgccg ggttgtacat
     4561 cacgtttatt gcccgaaggc cctttctcac gcaaccaggc tcttgccgtc acaacagctt
     4621 accttaatgt gcttattgaa gatgaccagg gaacgcattt ccggctggtg atccgcaacg
     4681 ctgaaggcca gttgcgctgg cggtgctgga attttgaacc tgatgccgga aaacagctaa
     4741 atccgtatct cgccagtgag ggaattctca ggcaataaac gtcattcact tgatccatca
     4801 ggccgcgtct tctccgaaag acgcggcttt ttcatttata ccgcttaatc attcataagg
     4861 agccaattat gcgattagcc agccgttttg gttatgtaaa tcagatccgc cgggagcgcc
     4921 cgctgacacg cgaagaactg atgtaccacg tcccgagtat ttttgcggaa gaccggcaca
     4981 cctcccgcag tgaacggtat gcgtacattc ccaccatcac cgtcctggaa aatctgcagc
     5041 gggaaggctt tcagccgttc ttcgcctgcc agacccgtgt gcgcgaccag agccgccggg
     5101 aatataccaa acacacgctg cgtctgcggc gggccggaca gataaccggt cagcatgtgc
     5161 ctgaaattat tctgctcaac tcccatgacg gttcatccag ctaccagatg ttacccggat
     5221 attttcgtgc catttgtacc aatggcctgg tctgcggtca gtcgctggga gaagtccggg
     5281 tgccacaccg gggaaacgtg gtggacaggg tcatagaagg tgcttacgaa gtggtgggcg
     5341 tgtttgaccg gattgaggaa aagcgtgatg ccatgcagtc gctggtcctg ccgccaccgg
     5401 cactccaggc gctggcacag gcggcgctga cttaccgtta tggtgatgaa catcagcccg
     5461 tcaccactgc cgacattctg acgccacgac gccgggagga ttacggtaag gacctgtgga
     5521 gtgcttatca gaccatccag gaaaatatgc tgaaaggcgg gatttccggt cgcagtgcca
     5581 gaggaaaacg tatccatacc cgggccattc acagcatcga taccgacatt aagctcaacc
     5641 gggcgttgtg ggtgatggca gaaacgatgc tggagagcat gcgctgatgc cttttccctg
     5701 aaagcgcagt tctgactcat gtccgtcccg gcccgcgact tcttccattc atttactttt
     5761 tataaggaat aatctcatga caaccgtctc gcataatttc accacacctt ctgtttccgt
     5821 aaccactgca tcagggaata accagtctca gttggttgcc accatagtcc ctgatgaaca
     5881 gcgcatcagc ttctggccgc agcactttgg cctcattcca cagtgggtca ccctggagcc
     5941 ccgtatcttc ggctggatgg accgtctttg cgaaaactac tgcgatggta tctggaatct
     6001 gtacaccctg aacaacggcg gggcatttat ggcccccgaa ccggatgatg atgatggaga
     6061 aacatgggta ctgttcaatg ccatgaacgg taaccgcgct gaaatgagcc cggaagctgc
     6121 cggtattgcc gcctgtctga tggcgtacag ccatcacgcc tgtcgtacgg agtgttatgc
     6181 catgacggtc cattattacc ggctgcggga ttacgccctg cagcatccgg aatgcagcgc
     6241 cattatgcgc atcatcgact gaatggaggc agggacaatg cagcagcttt cctttctgcc
     6301 cggagagatg acgcccggcg agcgcagtct cattctgcgg gccctgaaaa ccctggaccg
     6361 ccatcttcat gaacccggcg tggccttcac ctccacccga gcggcacggg aatggctgat
     6421 tctgaacatg gcgggactgg agcgtgaaga attccgggtg ctgtatctga acaaccagaa
     6481 tcagctgatt gccggtgaaa ccctctttac cggcaccatc aaccgcacgg aagtccatcc
     6541 ccgggaagtg attaaacgcg ccctgtacca caatgccgca gccgtggtgc tggcacacaa
     6601 tcacccgtcc ggtgaagtca cccccagtaa ggcagaccgc cttatcacgg aacgtctggt
     6661 acaggcactg ggcctggtgg atatccgggt gccggaccat ctgatagtcg gtggcagcca
     6721 ggttttctcc tttgccgaac atggtctgct ttaacccgtc acaaccacat cacacctgtt
     6781 ttcactttta tcttctgtct tcagaggtat cccattatga aaattatcac ccgtggtgaa
     6841 gccatgcgca ttcaccggca gcatcctgca tcccgtcttt ttccgttctg caccggcaaa
     6901 taccgctggc aaggcagcac tgaagcgtat accggtcgtg aagtacagga tattcccggt
     6961 gtgctggccg tgtttgccga acgccgtaag gacagttttg gcccgtatgt ccggctgatg
     7021 agcgtcaccc tgaactgatg ttccgtcacc cgggaaacga gtacgaccag atgacctcag
     7081 acgccgctgt ggatgaggcc attctcatca atgcatacat cttcaccgaa accggtcagt
     7141 gccccggctg acctgtcatc gttaaaccat tcttaaccac ttttcagaga ggattttatc
     7201 gtgtcagaca aactctccgg gataactcat cccgacgaca atcacgaccg cccctggtgg
     7261 gggcttccct gcacagtgaa accctgtttt ggcgcccgtc tggtgcagga gggtaaccgg
     7321 ttgcattacc ttgccgaccg cgccggtatc agcggccgct tcagcgacgc agatgcatac
     7381 catctggacc aggcctttcc gctgctgatg aaacaactgg aactcatgct caccagcggt
     7441 gaactgaatc cccgacatca gcattccgtc acgctgtatg caaaagggct gacctgcgaa
     7501 gccgacaccc tcggctcctg tggttacgtt tatctgactg tttatccgac accggcaacc
     7561 gcaaccacct cataaccccg aagctctcac ctttagcact accagttaca gagaacttaa
     7621 gatgaaaaca ttatccgaca cacatgtacg ggaggcatct cgctgcccgt ctcccgtcac
     7681 catctggcag acactgctca tccgactgct ggaccagcat tatggcctca cactgaatga
     7741 cacaccgttt gccgatgaac gtgtgattga gcagcatatt gaggcaggca tttcactgtg
     7801 tgatgcggtg aactttctcg tggaaaaata cgcgctggta cgaactgacc agccaggatt
     7861 cagcgcctgt acccgctctc agttaataaa cagcatcgat atcctccggg ctcgccgggc
     7921 aacaggcctg atgacccgcg acaattacag aatggtaaat aacattaccc agggtaagca
     7981 tccggaggcg caacaatgaa actggccctg acactggaag ccgacagcat taacgtgcag
     8041 gcactgaaca tggggcgcat tgtcgttgac gtcgatggca ttgagctcgc tgaactgatt
     8101 aacaaagtcg ctgaaaacgg ttattcactc catgtggtgg acgactccga ccaacagtca
     8161 acctgcacac caccaccgtt aacaaccctt accggcatac gctgcagcac cgcacatatc
     8221 acggaaacag acaacgcctg gttgtactcg ctgtcacacc agaccagtga cttcggcgaa
     8281 tcagaatgga ttcattttac aggtagcgga tatctgttac gtaccgatgc gtggtcatac
     8341 ccggttctgc ggcttaaacg cctggggctg tcaaaaatgt tccgccgtct ggttgtcaca
     8401 cttatccggc gttatggcgt cagtctcatt catctggatg ccagcgctga atgcctgccg
     8461 ggtttaccca ctttcaactg gtaaccagaa acaacatgaa atcattaacc actgaaacag
     8521 cgctggatat cctcattgcg tggctgcagg acaatatcga ctgcgaatcg ggaattatct
     8581 ttgacaacga tgaggataaa acggattcgg cagcactgtt gccctgtatc gaacaggccc
     8641 gggaggatgt ccgtactctt cgtcatctgc agcttctgca ccagaaccgg taaatatcac
     8701 tcaccatact tcattccact gacgccagcc tgaacagggc tggcgttttc atttatctgc
     8761 aaaaaggaaa aacgattatg tcaggtatca caatctcccg tcctgaagtg gtcaacgggc
     8821 atacggacgt tatctgttcc acctcaatcg gacacattct ggctgtacga aagaccacac
     8881 tgatgcaaat cgacacactt atccggcaac tgacagaaat ctcagcaatg acagaaagta
     8941 ttggaggtaa aaccgccccg gactgggcca tgaaacagaa ttttcgctgc ggttgctggc
     9001 tgatggagaa acccgaaaca gcaatgaaag ccattacccg gaatcttgac cgcgaaatct
     9061 ggcgtgacct gatgcaacgt tccgggatgc tgtcattaat ggatgcacag gcacgtgata
     9121 catggtaccg gtcactggaa tacgataatt ttccggaaat cagtgcggca aacattctga
     9181 gcacatttga acaactgcac cagaataagg atgaggtgtt tgagcgaggg gtgatcaacg
     9241 tcttcagggg actgagctgg aattacaaaa ccaactcacc ctgtaaattt ggcagtaaaa
     9301 ttatcgtcaa caacctggtg aggtgggacc ggtgggggtt tcattttatc accgggcaac
     9361 aggcagatcg acttgccgat ctggaaagaa tgctacacct gttcagcggc aaaccgatcc
     9421 ccg
//
LOCUS       DAZGXZ010000177         9254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000177 DAZGXZ010000000
VERSION     DAZGXZ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.177"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..4444
                     /gene="traI"
                     /locus_tag="ACHKJF_004671"
     CDS             <1..4444
                     /gene="traI"
                     /locus_tag="ACHKJF_004671"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFP0951907.1"
                     /translation="WMLTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQVV
                     TQAIAGLSERKVQFTYTDVLVRTVGILPPENGVIERARAGIDEAISREQLIPLDREKG
                     LFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIV
                     SGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLL
                     EGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKD
                     AGVNTYLWQGGEQRPATIISEPDRNVRYARLAGDFAVSVKAGEESVAQVSGVREQAIL
                     TQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDR
                     YVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKIPVADGERLRVTGKIPG
                     LRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGHSVSD
                     SAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKA
                     RAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTS
                     FTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLM
                     ERVPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVKMLPESE
                     RPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSM
                     VGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQ
                     TPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
                     QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVR
                     EKAGELGKEQVMVPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGL
                     ITLEDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTV
                     MAVSGDSVTLSDGQQTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGN
                     RKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAISKAVQKGTAHDVLEPGADREVMN
                     AERLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKS
                     AGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIAR
                     DNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAE
                     EAISRETERRADEIVRKMAEEKPDQPDGKTEQAVRAIAGQEQERASSSERETALPESV
                     LRESQRVQDAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            4464..5210
                     /gene="traX"
                     /locus_tag="ACHKJF_004672"
     CDS             4464..5210
                     /gene="traX"
                     /locus_tag="ACHKJF_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP0951908.1"
                     /translation="MTTDNTNTTRNDSLAARTGTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLEQTWMFLAGRGAFPLFALVWGLNLSRHANIRQSAINRLWGWAVIAQF
                     AYYVAGFPWYEGNILFAFAVAAQVLTWCETRSLWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRAERLALLACLLAVIPALNLASSDVAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWPGDFFPVFYACHLTVLGVLAV"
     gene            5269..6129
                     /locus_tag="ACHKJF_004673"
     CDS             5269..6129
                     /locus_tag="ACHKJF_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0951909.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            6232..6789
                     /locus_tag="ACHKJF_004674"
     CDS             6232..6789
                     /locus_tag="ACHKJF_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP0951910.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVFQRNIPLSHKKLSRALKAITRSESYLCAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            6943..7146
                     /locus_tag="ACHKJF_004675"
     CDS             6943..7146
                     /locus_tag="ACHKJF_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951911.1"
                     /translation="MAVSSGLSEHSGIYRYQRVGRHAVRQEIRGFFREAAEAWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            7884..8345
                     /locus_tag="ACHKJF_004676"
     CDS             7884..8345
                     /locus_tag="ACHKJF_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0951912.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQDYGRWSEKIMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQEEARQQKRGLWADSAPVPPWVWRSRK"
     gene            8448..8831
                     /locus_tag="ACHKJF_004677"
     CDS             8448..8831
                     /locus_tag="ACHKJF_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951913.1"
                     /translation="MNYQAVSELITSSNHNVLIVGDSASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRDALNASEHIIIFNCTGWPDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            9111..>9254
                     /locus_tag="ACHKJF_004678"
     CDS             9111..>9254
                     /locus_tag="ACHKJF_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012368889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0951914.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEAR"
BASE COUNT         2236 a         2156 c         2847 g         2015 t
ORIGIN      
        1 atggatgctg acgctgaagg aaaccgggtt cgacatccgg gcatatcgtg acgcggcgga
       61 tcagcgtgcg gagacccgca cgcaggcgcc cggggctgtt tcacaggagg ggccggatgt
      121 gcagcaggtg gtgacacagg ccattgccgg attaagtgaa cgaaaagtgc agtttacgta
      181 cacggacgtg ctggtcagaa cggtcggtat actgccgccg gaaaatggtg tgattgaacg
      241 ggcacgcgcc ggtatcgatg aggccatcag ccgtgagcag cttatccccc tcgatcgtga
      301 gaaggggctg ttcacgtccg ggattcatgt gctcgatgag ctgtcagtcc gggcactcag
      361 tcgtgacatc atgaaacaga accgggtgac cgtacatccg gagaaaagtg tcccccggac
      421 ggccggttac agcgatgccg tcagtgttct ggcacaggat cgcccgtcgc tggccattgt
      481 gtccgggcag ggcggtgcag ccgggcagcg tgagcgggtg gcggagctgg tcatgatggc
      541 ccgggagcag gggcgggagg tgcagattat cgctgctgac cgtcgctcgc agatgaacct
      601 gaagcaggat gaacggctgt ccggtgagct gataaccgga cgtcgtcagc tgctggaagg
      661 catggctttc acgccgggca gtactgttat cgttgaccag ggcgaaaaac tctccctgaa
      721 agagacgtta accctgctgg acggtgccgc acgtcataac gtacaggtcc tgataaccga
      781 cagtgggcag cgaaccggta caggcagtgc actgatggcc atgaaagatg ccggggtgaa
      841 cacatatctc tggcaggggg gagagcagcg accggcaacc atcatcagtg aaccggaccg
      901 taatgtccgc tatgcccggc tggccggaga ttttgcggtc agcgtgaaag ccggagaaga
      961 gagcgtggca caggtcagcg gagtacggga acaggccata ctgacacagg ccattcgcag
     1021 tgaactgaaa acgcagggcg tgctcggaca cccggaggtg accatgacgg cgctgtcacc
     1081 ggtctggctg gacagccgga gccgttatct gcgggatatg taccgcccgg ggatggtgat
     1141 ggagcagtgg aacccggaga cacgcagtca tgaccgctat gttatcgacc gggtgacggc
     1201 gcagagtcac agcctgaccc tgcgggatgc acagggcgaa acgcaggtgg tgcgtatttc
     1261 ctccctggac agcagctggt cgctgttccg gccggaaaaa ataccggtgg cagacggtga
     1321 gcgtctgagg gtgacaggga aaattcccgg actccgcgtc tccggcggtg accgcctgca
     1381 ggtggcatcc gtcagtgaag atgcgatgac ggttgttgtg ccgggacgtg ctgaaccggc
     1441 caccctgcct gtgagcgatt caccgttcac ggcactgaag ctggagaacg gctgggtgga
     1501 aacgcccggg cattccgtca gcgacagtgc gaaggttttt gcctccgtca cacagatggc
     1561 gatggacaac gccaccctga acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc
     1621 ctcactggat gaaacccgta ctgcggaaaa acttgcccgc catccctcct ttacggtggt
     1681 ttctgagcag ataaaggcgc gtgccggtga gacattgctg gaaaccgcta tcagtctgca
     1741 gaaagccggg cttcacacgc cggcacagca ggccattcat ctggcccttc ctgtgctgga
     1801 aagtaaaaac ctggccttca gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc
     1861 agaaggaacc agttttactg aactgggagg ggaaatcaat gcgcagataa aacgcggtga
     1921 tttgctgtat gtggatgtgg caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc
     1981 gtatgaggca gaaaagagca ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc
     2041 gctgatggag agggtacccg gcgaactcat ggagacactg acgtcggggc agcgtgccgc
     2101 cacccgcatg atactggaaa cgtccgaccg tttcacggtg gtgcagggtt atgccggtgt
     2161 gggtaagacc acacagttcc gggcggtgat gtcagccgtg aagatgctgc cggagagtga
     2221 gcgtccccgt gtcgtggggc tgggccccac gcaccgtgcg gtcggtgaga tgcgcagcgc
     2281 cggcgtggat gcacagacac tggcatcctt tctgcatgac acgcagctgc agcagcgcag
     2341 cggagaaacg ccggatttca gcaacacgct gttcctgctc gatgagagct ctatggtggg
     2401 caataccgac atggcacggg catacgccct gattgcggcc ggtggtggtc gtgccgtggc
     2461 cagcggtgac acggaccagc tgcaggctat cgcgcccggt cagcctttcc gtctccagca
     2521 gacgcgcagt gctgccgatg tggtcatcat gaaggagatt gtgcgtcaga cgccggaact
     2581 gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa agggcactgt ccgggcttga
     2641 gagtgtgaaa ccgtctcagg tgccacgtca ggagggcgca tgggcaccgg agcactccgt
     2701 gacggagttc agtcacagcc aggaagcgaa actggcagaa gcgcagcaga aggcgatgct
     2761 gaaaggcgag gcttttccgg atgtccccat gacactgtat gaagccattg tccgcgacta
     2821 taccggcaga acaccggaag cacgggagca gacgctgatt gtcacgcacc tgaatgagga
     2881 ccggcgcgta ctgaacagca tgattcatga tgtacgggaa aaggccggtg agctggggaa
     2941 agagcaggtc atggtgcctg tcctgaacac agcgaatata cgtgacggag agctgcgtcg
     3001 tctctccacc tgggagacac atcgggacgc acttgccctg gtggataatg tgtatcaccg
     3061 gattgccggt atcagtaagg atgacgggct gataaccctg gaggatgcgg aaggtaacac
     3121 gcggctgatt tcgccccggg aggcggtggc tgaaggtgtc acactgtaca ccccggacac
     3181 catccgggtg gggaccggtg accggatgcg cttcacgaag agtgaccggg agcgcggcta
     3241 tgtggccaac agcgtctgga cggtgatggc ggtttccggt gacagtgtca cgctttctga
     3301 cggacagcag acccgggtga ttcgccccgg ccaggagcag gcagagcaac atattgacct
     3361 ggcctatgcc atcaccgctc acggtgcgca gggggcgagt gaaacctttg ccatcgcgct
     3421 tgaaggcacg gaaggtaacc ggaaacagat ggccggcttt gagtcagcct atgtggccct
     3481 gtcgcgtatg aagcagcatg tgcaggtgta caccgataac cgtcagggct ggacggatgc
     3541 catcagcaaa gccgtacaga aaggaacggc ccatgatgtg ctggagccgg gggctgaccg
     3601 ggaggtcatg aatgcagagc ggttgttcag tacggcacgg gagctgcggg atgtggcagc
     3661 tggccgtgct gttctccgcc aggcgggact ggccgggggc gacagtccgg cacggtttat
     3721 tgcaccgggg cgcaaatatc cgcagccgta tgtggccctg ccggcttttg accgtaacgg
     3781 caagtccgcc ggtatctggc tgaacccact gaccacggat gacggaaacg ggctgcgggg
     3841 attcagtggt gaaggccggg tgaaaggcag cggggatgcg cagtttgtgg ccctgcaggg
     3901 cagccgtaac ggcgagagcc tgctggctga taatatgcag gatggtgtcc ggattgcccg
     3961 tgataacccc gacagcggtg tggtggtgag aatcgccggt gaaggtcgtc cgtggaatcc
     4021 cggtgccatc accggtggcc gcgtgtgggg ggatatcccg gacaacagcg tccagccggg
     4081 ggccggaaac ggtgagccgg tcacggcaga ggtgctggca cagcggcagg ctgaagaggc
     4141 catcagtcgt gaaacggaac gccgtgcgga tgagattgtc agaaaaatgg cagaggaaaa
     4201 acctgaccag ccggatggta aaacagagca ggctgtcagg gctattgccg ggcaggagca
     4261 ggaaagggct tcgtcatcag aacgggaaac tgcgctgccg gaaagtgtgc tgcgtgagtc
     4321 tcaacgggtg caggacgctg tccgggaggt tgcccgggaa aatctgctgc aggagcgact
     4381 tcagcagatg gagcgggata tggtccgtga cctgcagaaa gagaaaaccc tgggcggaga
     4441 ctgatacagg aagacaaacg ctgatgacaa cggataacac gaacacgaca cgtaacgatt
     4501 cactggctgc ccggaccggt acctggctgc agtcgttgct ggtctggtca cccggacagc
     4561 gggatatcat caaaacggtg gcactggtgc tgatggtgct ggaccatgca aaccgcattc
     4621 tgcatctgga gcagacgtgg atgtttctgg ccgggcgggg cgcgtttccg ctgtttgcgc
     4681 tggtctgggg actgaatctg tcccgtcatg cgaatatccg gcagtcggct attaaccggc
     4741 tgtggggatg ggctgtgatt gcccagttcg cgtattacgt tgccggcttt ccctggtatg
     4801 aggggaatat cctgtttgcc tttgcagtgg cggcacaggt gctgacgtgg tgtgagacgc
     4861 gcagcctgtg gcgtactgcc gcggccatac tcctgatggc actgtggggg cctttgtccg
     4921 gcaccagtta cggcattgcc gggctgctga tgctggcagt cagtcaccgg ctgtaccggg
     4981 cggaagacag agcggaacgt ctggcgctgc ttgcatgcct gctggccgtt attccggcac
     5041 ttaaccttgc ctccagtgat gtggcggccg tagctggtct tgtaatgacg gtgctgaccg
     5101 ttggtctggt gtcgtgtaca gggaaatcat taccccgttt ctggcccgga gattttttcc
     5161 cggtattcta cgcctgtcat cttactgtgc tgggcgttct ggctgtgtga ctggtatgat
     5221 gctggcgctg attctgctta ttaataaaca acataacaca ggatatttat gaaaattacg
     5281 gatcacaaac tccctgaagg tattgctctg acatttcgtg tcccggaagg aaatataaaa
     5341 catcctttga ttctcttatg ccatggattt tgcggtatcc ggaatgttct gctgccttcc
     5401 tttgcgaatg cgtttacaga agccgggttt gcaacgatta cttttgatta tcgtggattc
     5461 ggtgagagtg aaggggaacg aggtcgtctg gttccggcaa tgcagaccga agatattatt
     5521 tcagttatca attgggcaga aaagcaggta tgtattgata atcagcgtat aggattatgg
     5581 ggaacatcgc tgggtgggtg tcatgtattc aatgcagctg cgcaggataa aagagtgaaa
     5641 agtattgtca gccagctggc ttttgctgac ggtgaggttc tggttaccgg agaaatgaat
     5701 gaactggaga aagcttcttt tctgtccacg ctgaataaaa tggcagaaaa gaagaaaaat
     5761 accgggaaag agatgtttgt gggggttacc cgggtactga gtgataatga atcaaaggta
     5821 ttcttcgaga aggtaaaagg tcagtatcct gaaatggata taaaaatacc tttccttacg
     5881 gttatggaga cgttgcaata taaaccggca gagagcgcag ccaaagtaca gtgcccagtt
     5941 ttggttgtta ttgccggcca ggatagtgtt aatccaccag aacagggaag agccttatat
     6001 gatgctgttg cttctggtac taaggaatta tacgaggaag ctgacgcatg ccattatgat
     6061 atttatgaag gcgcgttttt tgaacgtgtg gcggcagtac agacgcaatg gtttaaaaaa
     6121 catctttaat tgtcggtatt aatatgtcag aggtcgttga ggtcagctta atatcatagt
     6181 gagtagtttc aacgacctgg gggacatatt ttcacaaagg tcagggcaat catgacagag
     6241 cagaaacgac cggtactgac actgaaacgg aaaacggaag gggaaacacc gacccggagc
     6301 cggaaaacca tcatcaatgt caccacgcca ccaaaatgga aggtgaaaaa gcagaaactg
     6361 gcggagaagg ctgcccggga agcagagctg gcggcgaaga aagcacaggc cagacaggcg
     6421 ctgtccattt atctgacgct gccctcgctg gatgaggcgg tgaacactct gaaaccctgg
     6481 tggccgggat tgtttgacgg taacacgccc cggcttcttg cctgtggtat ccgggaggtt
     6541 ttactggatg aggtgtttca gcggaatatc ccgctctctc ataaaaaact cagccgggcg
     6601 ctgaaagcca tcacccgttc ggaaagttat ctgtgtgcaa tgaaagccgg cgcctgccgc
     6661 tatgatacgg aaggctatgt gacagaacat atcacacagg aagaagaaca gtatgcgcag
     6721 gcgcgcctgg aaaaggtgcg cagacagaac cggatcaagg atgagctgcg ggcgatcctg
     6781 gcagaataat aaaaacgccc tctgtatgac agagggcaac agcgaaaaaa tcgggaaaac
     6841 agacatccag agttttgatg gagaaaaaca cccaaccgtt cagggatgtt tcataatgtt
     6901 tctgacataa tccgggagcc gggtcaagag gggggataaa aaatggctgt atccagtgga
     6961 ttatctgagc acagcggtat ttatcgctat cagcgcgtgg ggcgtcatgc cgtccgtcag
     7021 gagattcgtg gttttttcag ggaagccgct gaggcctggc agaatgcggc ccggctggca
     7081 ccctgtgaag catggcggga ttttgccctg aagagagcat ctgcatgccg gtgtaagtgc
     7141 cgataaaaat aacccccgtt gcggagcgcc gggggatcag acttatatgg cagatcgtta
     7201 ttattatagt ggtgtaagaa ggagttgccc tgtctttcaa acggacatca cagaattaac
     7261 atatttcaca tcccgggtaa gccaacaggg ataaaaaatg tgatgaattt caagaagcag
     7321 aaggtgacag tggcatgcat aacagtacgg ttatgtccgg tgaatctgac agacagccgg
     7381 gctgtattcg cggcaggata aagaaaaacc gggagatgac tctcccggtc cgtcttatcg
     7441 ctaatcccct gagcagggca tgtatgactt atctatgacc gttatattca gcgccacatc
     7501 cggtagcctg cgatataaca cagttatcct atcagtaaga gcaggtatga tgaatacacg
     7561 gagattaaaa atataatctc cgtcacatgt tacccccgga aaccggtaac gccgtcacgg
     7621 gaaatacgtg acagaaaaag cagcctccgt tactccaggt atcagaaggc tgaataatgc
     7681 atgagacttt cttatgtgtc tggtcaggcg gggtgaccag cgtttttagt aacacatagc
     7741 catatgtgta ttaagccacc tcatcttatt ttgtcgaatt gatcacaatt caacggttta
     7801 tttttggcgt gatgcgtgag ccgttattca tcccggcatg gaattcatgc aatcacgaaa
     7861 agtaatcagt gacggagagt aaaatgaaga aatatatacc agtggtatta tttgtttttt
     7921 catgtcaggt attttctgct gatattcatg gacgtgttgt gcgtgttctg gatggcgata
     7981 ccattgacgt catgttatct cagcatccgg tgcgtgtccg tcttgttaat attgatgcac
     8041 ctgaaaagaa acaggactat ggccggtggt ctgaaaaaat aatgaaatct ctggttgcgg
     8101 gaaaaacggt tactgtcaca tattttcagc gagatcgtta tggtcgtatt ctcgggcagg
     8161 tttatgcacc agacggtatg aatgttaatc agtttatggt ccgtgcaggc gctgcatggg
     8221 tttatgagca gtacaacacc gatcctgttc ttcccgtcct gcaggaagaa gcacgacagc
     8281 agaaacgtgg tctgtgggca gacagcgctc ctgttccgcc ctgggtatgg cgctcacgga
     8341 aataatgata tttctgtggc tattgagttc tcattgaaat gcgatattta aaagcatgaa
     8401 gcatattaca ttattaactt atttctctgt taatgaaagg tgaacaaatg aactatcagg
     8461 cagtatcaga attaatcaca tcgtctaatc ataatgttct tattgtgggg gactcagctt
     8521 ctgaagttga tagcttttta aataaactga atattactga ttataagtat tatgatttca
     8581 gccagattta cagttgttct gacagaacat taaatgatta tgcggttatt tttatcaggg
     8641 atgcactgaa cgccagtgag catattatta tttttaattg tacaggatgg cctgatttga
     8701 ataatgaatc tgccgtcatg cagtttgcca gagtggcacg aaaatcagga aaacaactga
     8761 tagtcgcagt aagagaacag gatatgaaaa aaatggaagc agagtttgga aggatcataa
     8821 aaatccactg acgccttcgc gttgctcagt tgtccaaccc cggggacggg aaaaagcaag
     8881 ttttccccgc ccccggcgtt tctgtagtga aaagcgggaa atggagggaa tggtcagtaa
     8941 cagggaatgt ggtattatcg cggcgggtgt ctgagccttt ctggttcagg caagacgcag
     9001 gtaccagaaa tgcgaagacc ccacttgtta atccattaac tcgtgaggcc tgcatgaagt
     9061 acttgaacac tactgattgt agcctcttcc ttgcagagag gtcaaagttt atgacgaaat
     9121 atgcccttat cgggttgctc gccgtgtgcg ccacggtgtt gtgtttttca ctgatattca
     9181 gggaacggtt atgtgagctg aatattcaca ggggaaatac agtggtgcag gtaactctgg
     9241 cctacgaagc acgg
//
LOCUS       DAZGXZ010000178         9231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000178 DAZGXZ010000000
VERSION     DAZGXZ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.178"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            20..301
                     /locus_tag="ACHKJF_004679"
     CDS             20..301
                     /locus_tag="ACHKJF_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951915.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(903..2363)
                     /gene="phoQ"
                     /locus_tag="ACHKJF_004680"
     CDS             complement(903..2363)
                     /gene="phoQ"
                     /locus_tag="ACHKJF_004680"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP0951916.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(2363..3034)
                     /gene="phoP"
                     /locus_tag="ACHKJF_004681"
     CDS             complement(2363..3034)
                     /gene="phoP"
                     /locus_tag="ACHKJF_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP0951917.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(3202..4572)
                     /gene="purB"
                     /locus_tag="ACHKJF_004682"
     CDS             complement(3202..4572)
                     /gene="purB"
                     /locus_tag="ACHKJF_004682"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP0951918.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAVVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(4576..5217)
                     /gene="hflD"
                     /locus_tag="ACHKJF_004683"
     CDS             complement(4576..5217)
                     /gene="hflD"
                     /locus_tag="ACHKJF_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP0951919.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(5253..6359)
                     /gene="mnmA"
                     /locus_tag="ACHKJF_004684"
                     /gene_synonym="trmU"
     CDS             complement(5253..6359)
                     /gene="mnmA"
                     /locus_tag="ACHKJF_004684"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP0951920.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(6413..6874)
                     /gene="nudJ"
                     /locus_tag="ACHKJF_004685"
     CDS             complement(6413..6874)
                     /gene="nudJ"
                     /locus_tag="ACHKJF_004685"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP0951921.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(6884..7537)
                     /gene="rluE"
                     /locus_tag="ACHKJF_004686"
     CDS             complement(6884..7537)
                     /gene="rluE"
                     /locus_tag="ACHKJF_004686"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP0951922.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            7709..8959
                     /gene="icd"
                     /locus_tag="ACHKJF_004687"
     CDS             7709..8959
                     /gene="icd"
                     /locus_tag="ACHKJF_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP0951923.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(9062..>9231)
                     /locus_tag="ACHKJF_004688"
     CDS             complement(9062..>9231)
                     /locus_tag="ACHKJF_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0951924.1"
                     /translation="KDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNS
                     CIIKICPYGLK"
BASE COUNT         2290 a         2326 c         2307 g         2308 t
ORIGIN      
        1 tcagagcgat gggggcacaa tgacagaagc cgaactactg ggattaatcc gccgcgtcgc
       61 cggaatcagc cagcagcatg acgaacaggc cacacagccg gacagcgtga ccgctgaaaa
      121 ttatgtgcgt gttgtagcgg aagtgatgcg ccgtgatggt atagaactta atggcgttga
      181 tatgcgcaac atacggaacc gcgttcttga aatgctggcc tacaatcgcc gcgttgaact
      241 gtatcgggag aaagaaaaaa taacgtacca ctggaagaag ccggaacggc tgcggcggta
      301 acttgctgat attcccgata acgcaaaatt gcgctggcta catccctcac atctgaggac
      361 tgttaaccag aaggccggaa tatattcaca aactacgaaa ttttcgtagt ttaattaatg
      421 gtcagattaa tcgctaaccc gctgatattt tcggaaacct caatttgagg aagtcggaag
      481 cgtcaccctt catattctga atggtcataa tgaccacgca gaccatcaag gcaaccacaa
      541 cgaacatcgt tacggttgga gcttttaccc cactggggga aaggtattac gataatcata
      601 acacctaccg aacaggtaaa acccaccagc ctgattaaca gttaaccgga aaaaaaatcc
      661 ggtagccccg ttatggggat ccccatatcg acattaacgc ccctcatgaa ttggtgcgct
      721 tccccctgga aagattaccc gcctgtatat ttcttgtgtc tatttgttcc acgttgtttc
      781 atacagtgca ccgaacggtg tagttaatgg tgtagtcaat ttgctgtttt tagcactttt
      841 tgcagtgtgg gttgtttaat taaatcaatg gtttgccttg ctgtggtgat aaaaggacat
      901 atttattcat ctttcggcgc agaatgctgg cgaccaaaaa tcacctccat ccgcgcaccg
      961 cccagcatgc tctctccggc gacgatttta ccctcatatt gctcggtgat ttcgcgggct
     1021 accgccagcc ctacaccttg cccagggcgt aaagtatcaa cccgttgacc acggtcgaaa
     1081 atgacctctc gcttgcttaa tggaataccg gggccatcat cctcgaccac aatatagaga
     1141 tgctcgtcgg tttgccttgc agaaatttcg acaaactcga ggcaatattt acaggcatta
     1201 tccagcacgt tgcccatcac ctcgacaaaa tcgttctgct caccgacaaa gctgatctct
     1261 ggcgaaatat cgagagagat attgacccct ttgcgttgat acactttgtt cagcgctgag
     1321 gtgagattgt ccagcagtgg ggcgaccgga tgcagctcgc ggctgagcaa tgtcccgccg
     1381 cgcatactgg cacgatgcag gtagtagcca atttgctgtg aaatgcggct gatttgctcc
     1441 agcattaccg gctcagcatc actgacgctc atcttttcac tacgcagaga acgcagcgta
     1501 ctttgcagca ccgccagtgg cgttttcaga ctatgggtca ggtcggtgag cgtcgtacgg
     1561 tatttgtcgt aacgttcgcg ttcacttttt aacaatcggt tcaggtttcg taccagactg
     1621 gtcagttctc gcgttgtggc tggattgagc aattcgcggt tatgttcttc cagttcgcgg
     1681 acttcttttg ccagggcttc gatggggcgt aaactccacc aggcggcgac ccacagcagc
     1741 gggatcacta acagcagatt ggctgagagc acatagataa accagctcca gaccatatag
     1801 gaacttttta gctccaccgg aatggtatcc accaccacaa tggttaattt tggcatccgc
     1861 gatgttgccg ggtagacgtt tactgccacc gagtgggtca tctccgcgtc gtcatcatct
     1921 tcccgcactt cctgcaactg ttgctgtatc gaatgatctc cactcagcaa gaggctggta
     1981 tcgttaacat ccgcttcaat ttcatgaaaa ccattcgatt tcagccagtc aggctggatc
     2041 atcttcatca gccagggcac gtcacgttgc gcccataaaa gctgcccgtt ctcatcataa
     2101 attagcgtca tggtggggct ttgcttgtcg atattttcgg gtaactcgac atgcaactta
     2161 ttgttttccc acttcgcaag ggtatagaac agattgctct cgccacgtaa cagccgaaac
     2221 gtagttttat cgaaactgac gctataaccg atcagcgcga ccattccgta ggcaagcgaa
     2281 agcaccagta ctaccgcagc cgttgccaac agaaaacgta cccgcagtga gagcgggaaa
     2341 aaaagacgca gtaatttttt catcagcgca attcgaacaa atagccctgg ccgcgaacgg
     2401 tagtaatcac ttcttgcgga tattgtgcct gaattttttt gcgcagacgt cccatcagta
     2461 catcaatggt atggctttcc cgcagctccg catccggata gagttggagc attaacgaat
     2521 ctttgctcac cactttgcca ttattgcgta tcaacgtttc cataatggtg tattcgaacg
     2581 cggtcagttt gatcacttcg tcattaatag ataattcacg gcgagagaga tcaacctgaa
     2641 acgggggtag cgaaatgacc tgtgaagcca ggccgctatt acgccgcatt aatgcctgca
     2701 ttcgcgccat cacctcttca atatgaaacg gtttagtcac gtaatcatca gcaccggcac
     2761 ttaatacttc gactttgtcc tgccagcttt cacgggcggt taataccaga atcggcagtg
     2821 aaacatcatt gctacgccag cggcgaatca gtgacagacc gtcctcgtca ggcaatccga
     2881 gatcgacaat cgcaatatcc ggtaaatgtt cattgagata ataatcggct tctttggcat
     2941 cttctgcgtc atcgacctga tgaccagcat cctgaatctg aactttaagg tggtgacgta
     3001 acaacgcatt gtcttcaaca accagtacgc gcatttttat ttctccctgt cttaattatt
     3061 aaaatagtgt aacgcgatta tgtgtttatg ggggtaaaca ttaaataaac cagcggggag
     3121 gggaggtaaa gtgaaaaaag aaaaagtgga taattttaat aaacaggccg gacagcatcg
     3181 ccatccggca ctgatacgag tttatttcag ctcatcaacc atggtgatag ctcgaccaat
     3241 atagttagcc ggcgtcatcg ctttcaggcg ggctttctct tcttctggca acgccagacc
     3301 atcgataaac tgcttcatgc cttcggcgtc aacgcgctta ccgcgagtca gctctttcag
     3361 cttctcgtac ggtttttcga tgccatagcg acgcataact gtctggattg gctcagccag
     3421 cacttcccag ttgtgatcca gctcatccag cagatggtca cggtttactt ccagtttgct
     3481 cacgcctttc agggtggatt gatacgcaat cagcgcataa ccgataccca cgccgaggtt
     3541 acgcagcacg gtggagtcgg tcaggtcacg ctgccagcgg gaaaccggca gtttgcttgc
     3601 cagatgctgc aataccgcgt tggaaaggcc cagattccct tcggagtttt cgaagtcaat
     3661 cgggttaact ttatgcggca tggtggaaga accaatctca ccagcaatgg ttttctgttt
     3721 gaagtggtta agggcgatat aaccccagac gtcacggtca aagtcgatca gaatggtgtt
     3781 gaagcgcgca acgcaatcaa acaattcggc aatgtagtcg tgcggttcga tctgggtggt
     3841 gtacgggttc cactgaatac ccagcgaggt gacgaactct tcgctgaact gatgccagtc
     3901 aacttccggg taagcggcga tatgggcgtt atagttaccg accgcgccgt tgattttgcc
     3961 gaggatctcc acctgattaa gctggcggta ctggcgctcc atacggtagg cgacgtttgc
     4021 catctcttta ccgatggttg acggcgtggc tggctgaccg tgggtacgag acagcagcgg
     4081 gatatcgcgg tactgaacgg cgagatcttt aatgccatca atcagttgac gccagtacgg
     4141 caggatcact tcatcacgcg cggttttcag cattaatgcg tgggagaggt tattgatatc
     4201 ttccgaagta caggcaaagt ggatgaattc agaaaccgcg tgcagttccg ggatctccgc
     4261 cactttttct ttcaggaaat actcaaccgc tttaacgtcg tggttagtgg tacgctcgat
     4321 ggttttgatg cgtgcggcat cttcttcgct gaaattggcg acaactgcat caaggtaacc
     4381 gattgcgtcg gcagcaaaag caggaacttc cttgatcgct gcgtgcgcgg ccagtttttg
     4441 cagccaacgt acttcaactt gtacacggaa tttcagcaaa ccatattcgc tgaaaatccc
     4501 gcgcagcgcg ctgactttat cgccgtagcg tccatcgaca ggggatacgg cggtcagtga
     4561 ggataattcc atagatcaca actccggggt taaatgagca agaatttgtt ttgcctgagt
     4621 ggtcaggcga ttacgagaaa acatcagttg cagacgtccg ccgccgacct ggtgccagag
     4681 cacggcggcg cgaatgcctg ccagcagggt tgcgcgaact ttcgcctgca cttgtgggct
     4741 ttgcagtaca gcaggggaac cggtgacctg aatgcgcggg ccaagcgggc tgatcacatc
     4801 aacatagata gcagccatcg cgctcatcag cgtttcggac tgtaaatcga agtgttcgag
     4861 ctggcgttgc aggccgttga tccggttgcc cagagtgtcg agcgcgcctt tcgctgagga
     4921 gagtttgcgc tcaagcacca tcaagctgag tgtgtagcgg gttaattcgg cgtttaagcc
     4981 ctggcgactg ctggcattga gcacgccgag cagggtttcc agcccgacgc gcaggttggc
     5041 ttcgctaccg ccaaaaaccg ccagcgtcga gctggggttc atatcaataa tactgttgag
     5101 tgagacgtgt agcgcatcgg catcacaatg cccctggtga gcgagttgtt gcaccaggcg
     5161 tgccgactga caaataccgg ccagggcgag ggtgatgtca tagtaattct ttgccacgtt
     5221 cactgcttcc ttgttttaag taaagataat aatcagaccg gcagcggcag acgctgctca
     5281 ataataccgc caccgaggca cacttcaccg ttatagaaga cggcagactg gcccggcgtc
     5341 acggcggcaa ccggttcatc gaaaatcact tcaatgcgat catcgtccag cgccttgacg
     5401 gtgcaaggga tatcggtctg gcgatagcgg gtttttaccg tgcaacgcat agtgccggtg
     5461 aatggttcgc gatcgaccca gtgcaactgc tgggcaatca acccgacaga catcagccgc
     5521 gggtgttcat ggccctgagc gacaaccaga atgttgtttt cgacgtcttt gtccaccaca
     5581 taccacggtt cttcggtacc ttctttggtg ccaccgatac ccagaccttt acgctgaccg
     5641 agagtgtgat acatcagccc ctggtgctca ccaatttcat cgccatcgac ggtaatgatt
     5701 ttgcccggtt gcgccgggag ataacggccc aggaactcgc ggaatttacg ttcgccaatg
     5761 aagcaaatgc cggtagagtc ttttttcttc gcggtgacca gaccaagatc ttcagcaatc
     5821 ttacgcacct gcggtttttc cagttcgccg accgggaaca ggctttgcgc aatctgctca
     5881 tggctgagcg tataaaggaa gtagctctgg tctttattgc tgtccagacc acgcagcagg
     5941 cggctcttgc catcgacatc ggcccgacgt acgtaatgac cggtagcgat ataatcggca
     6001 cctaaatctt cggcggcaaa ttcgaggaag gctttaaatt tgatctcttt gttgcacaga
     6061 atatccggat tcggcgtgcg accggcttta tattcggcaa ggaacagttc gaagacgttg
     6121 tcccagtact cggcagcaaa gttaacggtg tgcagttcaa tgccgagctt gtcgcagaca
     6181 gcctgggcat cagccagatc cgctgccgct gtgcaatatt cctcaccgtc gtcttcttcc
     6241 cagttcttca taaacaggcc ttcaacctga tatccctgtt gttgcaacag ccaggcagaa
     6301 acggaggaat cgacaccgcc ggacatgccg acgattactt tttttgcggt ttcagacatt
     6361 ggatcactca cgacattgaa cttcaaggcg gcgtattcta tcacgcagcc ttttagatga
     6421 caccctttgt aaaaggccag ttaaaatcgc caatcatctc cagcggatag cgttgcccgc
     6481 tttgataaca acggatactt tccgccacca gcggcgaacg aagatttgac gcctttaaaa
     6541 tctcttcggc gctgacccaa cgacagcagt cgatatcgct gtcatgaggc tgggttgggc
     6601 atatttgctc aagctcaatg gcaaagagga aacgcaaaaa cggcgtttta tctggcgcaa
     6661 tccactgatg catacgaata aagtgttgcg gctgcgcgct gatgccggtt tcttcccaca
     6721 gttcacgggc ggcggcttcc actaaggttt catcggcttc cagatgcccg gcaggttggt
     6781 tccataacgc tttaccatta atcgtctctt caacgactaa aaatttgcct tctgcgtgca
     6841 ccacgcaagc aacggtaacg tgcggtttaa acattttgca tccttaatct gtcacttctc
     6901 gccattcgcc attggcaaga ttatccaaag agtaatctcc catcgcatag cgaatcagtc
     6961 gcagcgtggg gaaaccaaca tgggcggtca tgcggcgcac ctggcgatta cgtccttcat
     7021 ataaggtgat cttcagccag ctggtgggaa tacttttgcg ttcacgaatt ggtggattcc
     7081 gcggccataa ccacgcgggt tcgtcaacca gctccgcgcc ggcgggcagg gtagggccat
     7141 catttaaggt tacgccattg cgcaaggctt caagtgcgtc ttgtgtggga ataccttcca
     7201 cctgcacata atagattttc ccggtgcgtt tacccggctg ggttaaacgc gcctgcaacg
     7261 cgccgttatt ggtcagcacc agtaaccctt cgctatcgcg gtcaagacga cctgctgcat
     7321 aaacgccctg aaccgggatg aattctttta atgttttgcg tccggcttca tcggtgaact
     7381 gcggaagaac atcgtagggt ttattgaaca ggatcacacg cgtgggctgg ttttcaggtt
     7441 tacgcctggt agaacgttgc gagctgaatc gcttaacctg gtgatttcta aaagaagttt
     7501 tttgcatggt attttcagag attatgaatt gccgcattat agcctaataa cgcgcatctt
     7561 tcatgactgc aaacaatagg gtagtattga caagccaatt acaaatcatt aacaaaaaat
     7621 tgctctaaag catccgtatc gcaggacgca aacgcatatg caacgtggtg gcagacgagc
     7681 aaaccagtag cgctcgaagg agaggtgaat ggaaagtaaa gtagttgttc cggcacaagg
     7741 caagaagatc accctgcaaa acggcaaact caacgttcct gaaaatccga ttatccctta
     7801 cattgaaggt gatggaatcg gtgtagatgt aaccccagcc atgctgaaag tggtcgacgc
     7861 tgcagtcgag aaagcctata aaggcgagcg taaaatctcc tggatggaaa tttacaccgg
     7921 tgaaaaatcc acacaggttt atggtcagga tgtctggctg cctgctgaaa cccttgatct
     7981 gattcgtgaa tatcgcgttg ccattaaagg tccgctgacc actcctgttg gtggcggtat
     8041 tcgctctctg aacgttgccc tgcgccagga actggatctc tacatctgcc tgcgtccggt
     8101 acgttactat caaggcactc caagcccggt taaacaccct gaactgaccg atatggttat
     8161 cttccgtgaa aactcggaag acatttatgc gggtatcgaa tggaaagcag actctgccga
     8221 cgccgagaaa gtgattaaat tcctgcgtga agagatgggc gtgaagaaaa ttcgcttccc
     8281 ggaacattgc ggtatcggta tcaagccgtg ttctgaagaa ggcaccaaac gtctggttcg
     8341 tgcagcgatc gaatacgcaa ttgctaacga tcgtgactct gtgactctgg tgcacaaagg
     8401 caacatcatg aagttcaccg aaggcgcgtt taaagactgg ggctaccagc tggcgcgtga
     8461 agagtttggc ggtgaactga tcgacggcgg cccgtggctg aaagttaaaa acccgaacac
     8521 cggcaaagag atcgtcatta aagacgtgat tgctgatgca ttcctgcaac agatcctgct
     8581 gcgtccggct gaatatgatg ttatcgcctg tatgaacctg aacggtgact acatttctga
     8641 cgctctggca gcgcaggttg gcggtatcgg tatcgcccct ggagcaaaca tcggtgacga
     8701 atgcgccctg tttgaagcca cccacggtac tgcgccgaaa tacgccggtc aggacaaagt
     8761 aaaccctggc tctattattc tctccgctga gatgatgtta cgccatatgg gctggactga
     8821 agccgcagac ctgattgtta aaggtatgga aggcgcaatc aatgcgaaga ccgtaaccta
     8881 tgacttcgag cgtctgatgg aaggcgctaa actgctgaaa tgttcagaat ttggtgatgc
     8941 gatcatcaag aacatgtaat cactacatgt gtttaatatt gcaacgggcg tataacacgc
     9001 ccgttgtttt atttatgtag gtattattaa tagcatatcg agcatattta tataaaaccc
     9061 tttacttgag cccatatggg catattttta taatgcaact attatgcaaa catttatttg
     9121 ttattttgct ttctcctgga ggacactctt gactgctttt gagtagactc cataaatcct
     9181 tgttgaatgg tgcgatgtta taaatagtaa taggatattc tttatcttta a
//
LOCUS       DAZGXZ010000179         9118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000179 DAZGXZ010000000
VERSION     DAZGXZ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.179"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..186)
                     /locus_tag="ACHKJF_004689"
     CDS             complement(<1..186)
                     /locus_tag="ACHKJF_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951925.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPY"
     gene            complement(204..422)
                     /locus_tag="ACHKJF_004690"
                     /pseudo
     CDS             complement(204..422)
                     /locus_tag="ACHKJF_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            665..1660
                     /locus_tag="ACHKJF_004691"
     CDS             665..1660
                     /locus_tag="ACHKJF_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0951926.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYATMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNDHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            complement(1668..3101)
                     /gene="cscA"
                     /locus_tag="ACHKJF_004692"
     CDS             complement(1668..3101)
                     /gene="cscA"
                     /locus_tag="ACHKJF_004692"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP0951927.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            3317..4231
                     /locus_tag="ACHKJF_004693"
     CDS             3317..4231
                     /locus_tag="ACHKJF_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP0951928.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGI
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDQDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            4303..5550
                     /locus_tag="ACHKJF_004694"
     CDS             4303..5550
                     /locus_tag="ACHKJF_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP0951929.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(5991..6065)
                     /locus_tag="ACHKJF_004695"
     tRNA            complement(5991..6065)
                     /locus_tag="ACHKJF_004695"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6030..6032),aa:Arg,seq:cct)
     gene            complement(6141..7073)
                     /locus_tag="ACHKJF_004696"
     CDS             complement(6141..7073)
                     /locus_tag="ACHKJF_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP0951930.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            7368..8123
                     /gene="mlaA"
                     /locus_tag="ACHKJF_004697"
     CDS             7368..8123
                     /gene="mlaA"
                     /locus_tag="ACHKJF_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP0951931.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(8305..>9118)
                     /locus_tag="ACHKJF_004698"
     CDS             complement(8305..>9118)
                     /locus_tag="ACHKJF_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951932.1"
                     /translation="DTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHDINN
                     KEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKGDSL
                     AIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSKAIM
                     AESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKFRRDNVKEIISNL
                     FANKVDVDHPENKHKDFKDLEDNVEKRLQNRQAKYQNEINQLSAPGVNFDDI"
BASE COUNT         2194 a         2078 c         2217 g         2629 t
ORIGIN      
        1 atagggtgca aaacgggata agcgcgcatg ggcgtcctga acatcctgtt cggtcaggcc
       61 aacataaggt aaaccttcag ccaatgaggt cgtgccagga ttaaaccagg tggtttcttt
      121 aagagcaacc agatccttta ccaacggata ctgggcgatg agcgagttca ttttagcgtt
      181 ttccatagta cgtctctttg cgcttagatg ataaatgaca gcaggaatgt gcccgccaga
      241 gcgacgactg aagcgataaa tgtcgctgtc gtatagtatt taaatgtttc attgagcgta
      301 gcgccgcaat attgcttcac tagccagaaa agcgagtccg taacgatcgt gcagccaatt
      361 gcacctgaac cgatagcaat cgcaataatt tccgggctga tgtcgggata cagcggcagc
      421 acaatgatcc atatttatat tttcttctcc aggttggcga tgccagttgg catgttttac
      481 ttacttgcct atatggttac tcgttggcga gaacagaggc ggaattgagt acaattatcc
      541 ggcaattatt gttatttttt attgtattgc cgtgtcgggt gatagggcgt tgagaatatc
      601 gttggatctc gccagtgacg tctgtttctg ctacagtgcc cgttttacgg caaacggctt
      661 gggtatggct tcattaaagg atgtcgcacg cctggcggga gtgtcgatga tgacagtctc
      721 ccgggtgatg cataatgcag aatctgtgcg tcctgcaacg cgtgaccgcg tattgcaggc
      781 aatccagacc ctgaattatg ttcctgatct ttccgcccgt aagatgcgcg ctcaaggacg
      841 taagccgtcg actctcgccg tgctggcgca ggacacggct accactcctt tctctgttga
      901 tattctgctt gccattgagc aaaccgccag cgagttcggc tggaatagtt ttttaatcaa
      961 tattttttct gaagatgacg ctgcccgcgc ggcacgtcag ctgcttgccc accgtccgga
     1021 tggcattatc tatgctacaa tggggctgcg acatatcacg ctgcctgagt ctctgtatgg
     1081 tgaaaatatt gtattggcga actgtgtggc ggatgaccca gcgttaccca gttatatccc
     1141 tgatgattac actgcacaat atgaatcaac acagcatttg ctcgcggcgg gctatcgtca
     1201 accgttatgc ttctggctac cggaaagtgc gttggcaaca gggtatcgtc ggcagggatt
     1261 tgagcaggcc tggcgtgatg ctggacgaga tctggctgag gtgaaacaat ttcacatggc
     1321 aacaggtgat gatcactaca ccgatctcgc aagtttactc aatgaccact tcaaatcggg
     1381 caaaccagat tttgatgttc tgatatgtgg taacgatcgc gcagcctttg tggcttatca
     1441 ggttcttctg gcgaaggggg tacgaatccc gcaggatgtc gccgtaatgg gctttgataa
     1501 tctggttggc gtcgggcatc tgtttttacc gccgctgacc acaattcagc ttccacatga
     1561 cattatcggg cgggaagctg cattgcatat tattgaaggt cgtgaagggg gaagagtgac
     1621 gcggatccct tgcccgctgt tgatccgttg ttccacctga tattatgtta acccagtagc
     1681 cagagtgctc catgttgcag cacagccact ccgtgggagg cataaagcga cagttcccgt
     1741 tcttctggct gcggatagat tcgactactc atcaccgctt ccccgtcgtt aataaatact
     1801 tccacggatg atgtatcgat aaatatcctt agggcgagcg tgtcacgctg cgggagggga
     1861 atactacggt agccgtctaa attctcgtgt gggtaatacc gccacaaaac aagtcgctca
     1921 gattggttat caatatacag ccgcattcca gtgccgagct gtaatccgta atgttcggca
     1981 tcactgttct tcagcgccca ctgcaactga atctcaactg cttgcgcgtt ttcctgcaaa
     2041 acatatttat tgctgattgt gcggggagag acagattgat gctgctggcg taacgactca
     2101 gcttcgtgta ccgggcgttg tagaagtttg ccattgctct ctgatagctc gcgcgccagc
     2161 gtcatgcagc ctgcccatcc ttcacgtttt gagggcattg gcgattccca catatccatc
     2221 cagccgataa caatacgccg accatccttc gctaaaaagc tttgtggtgc ataaaagtca
     2281 tgcccgttat caagttcagt aaaatgcccg gattgtgcaa aaagtcgtcc tggcgaccac
     2341 attccgggta ttacgccact ttgaaagcga tttcggtaac tgtatccctc ggcattcatt
     2401 ccctgcgggg aaaacatcag ataatgctga tcgccaaggc tgaaaaagtc cggacattcc
     2461 cacatatagc tttcacccgc atcagcgtgg gccagtacgc gatcgaaggt ccattcacgc
     2521 aacgaactgc cgcgataaag caggatctgc cccgtgttgc ctggatcttt cgccccgact
     2581 accatccacc atgtgtcggc ttcacgccac actttaggat cgcggaagtg catgattcct
     2641 tctggtggag tgaggatcac accctgtttc tcgaaatgaa taccatcccg actggtagcc
     2701 agacattgta cttcgcgaat tgcatcgtca ttacctgcac catcgagcca gacgtgtccg
     2761 gtgtagataa gtgagaggac accattgtca tcgacagcac tacctgaaaa acacccgtct
     2821 ttgtcattat cgtctcctgg cgctagcgca ataggctcat gctgccagtg gatcatatcg
     2881 tcgctggtgg catgtcccca gtgcattggt ccccagtgtt cgctcatcgg gtgatgttga
     2941 taaaacgcgt gataacgatc gttaaaccag atcaggccgt ttggatcgtt catccatccg
     3001 gcaggtggcg cgaggtgaaa atggggatag aaagtgttac cccggtgctc atgaagtttt
     3061 gctagtgcgt tttgcgccgc atgcaatcga gattgcgtca ttttaatcat cctggttaag
     3121 caaatttggt gaattgttaa cgttaacttt tataaaaata tagtccctta ctttcataaa
     3181 tgcgatgaat atcacaaatg ttaacgttaa ctatgacgtt ttgtgatcga atatgcatgt
     3241 tttagtaaat ccatgacgat tttgcgagaa agaggtttat cactatgcgt aactcagatg
     3301 aatttaaggg aaaaaaatgt cagccaaagt atgggtttta ggggatgcgg tcgtagatct
     3361 cttgccagaa tcagacgggc gcctactgcc ttgtcctggc ggcgcgccag ctaacgttgc
     3421 ggtgggaatc gccagattag gcggaataag tggatttata ggtcgggtcg gtgatgatcc
     3481 ttttggggcg ttaatgcaaa gaacgctgct aactgaggga gtcgatatca cgtatctgaa
     3541 gcaagatgaa tggcaccgga catccacggt gcttgtcgat ctgaacgatc aaggggaacg
     3601 ttcatttacg tttatggtcc gccccagtgc cgatcttttt ttagagacga cagacttgcc
     3661 ctgctggcga catggcgaat ggttacatct ctgttcaatt gcgttgtctg ccgagccttc
     3721 gcgtaccagc gcatttactg cgatgacggc gatccggcat gccggaggtt ttgtcagctt
     3781 cgatcctaat attcgtgaag atctatggca agacgagcat ttgctccgct tgtgtttgcg
     3841 gcaggcgcta caactggcgg atgtcgtcaa gctctcggaa gaagaatggc gacttatcag
     3901 tggaaaaaca cagaacgatc aggatatatg cgccctggca aaagagtatg agatcgccat
     3961 gctgttggtg actaaaggtg cagaaggggt ggtggtctgt tatcgaggac aagttcacca
     4021 ttttgctgga atgtctgtga attgtgtcga tagcacgggg gcgggagatg cgttcgttgc
     4081 cgggttactc acaggtctgt cctctacggg attatctaca gatgagagag aaatgcgacg
     4141 aattatcgat ctcgctcaac gttgcggagc gcttgcagta acggcgaaag gggcaatgac
     4201 agcgctgcca tgtcgacaag aactggaata gtgagaagta aacggcgaag tcgctcttat
     4261 ctctaaatag gacgtgaatt ttttaacgac aggtaggtaa ttatggcact gaatattcca
     4321 ttcagaaatg cgtactatcg ttttgcatcc agttactcat ttctcttttt tatttcctgg
     4381 tcgctgtggt ggtcgttata cgctatttgg ctgaaaggac atctagggtt gacagggacg
     4441 gaattaggta cactttattc ggtcaaccag tttaccagca ttctatttat gatgttctac
     4501 ggcatcgttc aggataaact cggtctgaag aaaccgctca tctggtgtat gagtttcatc
     4561 ctggtcttga ccggaccgtt tatgatttac gtttatgaac cgttactgca aagcaatttt
     4621 tctgtaggtc taattctggg ggcgctcttt tttggcctgg ggtatctggc gggatgcggt
     4681 ttgcttgaca gcttcaccga aaaaatggcg cgaaattttc atttcgaata tggaacagcg
     4741 cgcgcctggg gatcttttgg ctatgctatt ggcgcgttct ttgccggcat attttttagt
     4801 atcagtcccc atatcaactt ctggttggtc tcgctatttg gcgctgtatt tatgatgatc
     4861 aacatgtgtt ttaaagataa ggatcaccag tgcgtagcgg cggatgcggg aggggtaaaa
     4921 aaagaggatt ttatcgcagt tttcaaggat cgaaacttct gggttttcgt catatttatt
     4981 gtggggacgt ggtctttcta taacattttt gatcaacaac tttttcctgt cttttatgca
     5041 ggtttattcg aatcacacga tgtaggaacg cgcctgtatg gttatctcaa ctcattccag
     5101 gtggtactcg aagcgctatg catggcgatt attcctttct ttgtgaatcg ggtagggcca
     5161 aaaaatgcat tacttatcgg tgttgtgatt atggcgttgc gtatcctttc ctgcgcgctg
     5221 ttcgttaacc cctggattat ttcattagtg aagctgttac atgctattga ggttccactt
     5281 tgtgtcatat ccgtcttcaa atacagcgtg gcaaattttg ataagcgcct gtcgtcgacg
     5341 atctttctga ttggttttca aattgccagt tcgcttggga ttgtgctgct ttcaacgccg
     5401 actgggatac tctttgacca cgcaggctac cagacagttt tcttcgcaat ttcgggtatt
     5461 gtctgcctga tgttgctatt tggcattttc ttcctgagta aaaaacgcga gcaaatagtt
     5521 atggaaacgc ctgtaccttc agcaatatag acgtaaactt ttccggttgt tgtcgatatc
     5581 tccctatccc tcaaccggaa aataataata ctaaagtgct tagccctgct aataatcacc
     5641 taatccaaac gcctcattca tgttctggta cagtcgctca aatgtacttc agatgcgcgg
     5701 ttcgctgatt tccaggacat tgtcgtcatt cagcgacctg tcccgtgtat cacggtcctg
     5761 cgaattcatc aaggaatgca ttgcggagtg aagtatcgag tcacgccata tttcgctatc
     5821 aggattctgt gtgatggtta catcgcccgg cccagggctg tttagtcatc agcgctttct
     5881 gacagtgctg agatttcaac ctgttgcagt aaaaatgagt agatataagg caagtgtgct
     5941 gccaaaccta tcttttacgg ggtgaaggta gatttcgttt gaagggtatc tggtgtcccc
     6001 tgcaggaatc gaacctgcaa ttagccctta ggaggggctc gttatatcca tttaactaag
     6061 aggacaatgc ggcatgagta tacccgctaa tggactgcgg ggtaagtacg ctgccgctcg
     6121 attgcttaaa ccctcgccat ttatgctggg tttttatcat ttttcttaat gttttccgca
     6181 cgttctgctt tttggcgtgc ttctgcttta cgcttgttgc tcatgtcgtt acgaatctgt
     6241 gcatgactca ttaacgcgaa gataaaggtg ccgccgcaga tgttccccgc taaagtaggt
     6301 agtgcgaagg gccagatgaa atcgctccag tgcagcgtgc cgttaaacac cagatagagg
     6361 atttcaacag aaccgacaac gatgtgggtg gtgtcaccca gtgcaataag ccaggtcatc
     6421 aatataatca ccacaatctt tgccgcaccc gcagcaggaa acatccaaac catagtggcg
     6481 atcagccagc cggaaatgat cgcgttggca aacatctcgc tgggggtgtt cttcatcaca
     6541 tccatgccga ttttgacaaa tgcatcgcga gtttcttcat tgaagatagg catatattca
     6601 aatgcccatg cagcaatacc tgtcccgaga atattaccca gcagcacgac gccccataat
     6661 cgcataagta agccgacgtt gctcattgtc ggtttttgca tgacgggtag taccgcagtc
     6721 acggtgtttt cggtaaataa ttgctggcgg gccataatga cgataataaa accaaaggta
     6781 taaccgagat tctccagtaa gaagctgccc ggcacacctt ccagttcgac atgaaatatc
     6841 ccttttgcca gtaacgaagc gcccatcgac agacccgccg caatggctga ccacagtagc
     6901 gccattgcgt cgcgttccag ctctttttca ccatcctggc ggatatgctc atgaattgcc
     6961 atcgcccggg aggggagtcg gtcttcatct atttctattt ttttgccgcg ctctttttct
     7021 tcgctctcaa cttcaatttc gtcgctgtgt tgatcaattt tgtcgttgtc catggtctct
     7081 tctcttgaat tagcacgtat agctaaagcg tagcggcttt tttgctcgca actggcggga
     7141 gttactctga aaatgtagaa aaggctgcgt ttgccttttt ctgtttctat agaatcaagt
     7201 agcctacagg gcggcgatta ccaggctatg atcaaatcag caaatcaggg cgtctggaca
     7261 tcagttgacg tgctgttaca atcgcccaca cctaaacagg cggatacggt atcgttccgt
     7321 catggatggc aaactgcata agccataaaa aaaacaggga gacatttatg aagcttcgcc
     7381 tgtcggcgct tgctctggga actacgcttc tggtggggtg tgcgagttcc ggtacagatc
     7441 agcaagggcg ttctgacccg ttagaagggt tcaaccgcac catgtacaac ttcaacttca
     7501 acgtattaga cccgtatatt gttcgaccgg tcgctgtcgc ctggcgtgat tatgttccgc
     7561 aaccggcgcg taatggtttg agcaacttta ctggcaacct tgaagaacct gcggtgatgg
     7621 ttaactactt cttgcagggc gacccttatc aggggatggt ccactttacc cgctttttcc
     7681 tgaacaccat tttggggatg ggcggcttta ttgatgttgc agggatggcg aacccgaaac
     7741 tgcaacggac tgaacctcac cgcttcggta gtacgcttgg tcattatggc gtgggttatg
     7801 ggccttacgt tcagttaccg ttctacggta gcttcacgct gcgtgatgac ggtggtgata
     7861 tggcggatgg tctttacccg gttctttcct ggctgacctg gccgatgtct gtgggtaaat
     7921 ggacgcttga agggatcgaa acccgcgctc agctgctgga ttccgatggt ctgctgcgtc
     7981 agtcgtccga tccttatatt atggtgcgcg aagcgtactt ccagcgtcat gatttcatcg
     8041 ctaatggcgg cgaactcaaa ccgcaggaaa atccgaacgc acaagcgatt caggatgatt
     8101 taaaagatat tgattctgaa taagaaacaa ataaaaaagg tgagtcgcaa aactcacctt
     8161 ttttgttgtt atccctcaat taccacaagt aaaaggagat aaaccgacaa tgacccggac
     8221 gtaatgatgt aaaaatactg atgttcgtca ctgactattt ccgatattca ggcgttgaaa
     8281 aatcaaaaaa ctggaaaaat aattttaaat atcatcaaaa ttaacacctg gcgcagatag
     8341 ttgattaatt tcattttgat atttcgcctg gcgattttga agtctttttt caacgttatc
     8401 ttcgagatcc ttaaagtctt tgtgtttatt ttcaggatgg tcaacgtcaa ctttgttagc
     8461 gaataaatta gaaattattt cttttacatt gtcgcgtcta aattttgcat cgtccacttg
     8521 aaaattatat cttattgctc gacctaaacg tttaaaaact tcgttaggtt ctctcagtct
     8581 ttccccgttg agctcgttta caacctgatg atcggaacct gttgcaaatg attctgccat
     8641 gatagcttta ctgctttcaa accacgctga agcattcctg gctttttcac tacaatattg
     8701 ggtgagacta tcaccattaa tacgaattcc tctttgaatg tggtagtcgt cctgatcgta
     8761 gtaaccgaga gataagtaat catttccgtt tttattttta attacaatag cgagagagtc
     8821 gcctttttta tctttaccgt accaggtaaa taagtccgtt ggttcgctat tcccgctctc
     8881 atcaaattcc gttaccgtat gtctgagttt gtgtgatatc ttatcgaaga taaaaccagg
     8941 atctccatca tatatttctt ttaatgcgct gaagatcatt tccttgttat tgatatcatg
     9001 agtcttctca actatgttgt cattaatgat agtacggacg agttctttgt ctttatagtt
     9061 cgcaggtaat tctattggtt cttggttgtc gataccaata gtaatatggt ctgtatca
//
LOCUS       DAZGXZ010000180         9062 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000180 DAZGXZ010000000
VERSION     DAZGXZ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.180"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            49..321
                     /locus_tag="ACHKJF_004699"
     CDS             49..321
                     /locus_tag="ACHKJF_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP0951933.1"
                     /translation="MALPRITQKEMTERKQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(454..1326)
                     /gene="rluF"
                     /locus_tag="ACHKJF_004700"
     CDS             complement(454..1326)
                     /gene="rluF"
                     /locus_tag="ACHKJF_004700"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP0951934.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRAAIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1538..2227
                     /gene="pepE"
                     /locus_tag="ACHKJF_004701"
     CDS             1538..2227
                     /gene="pepE"
                     /locus_tag="ACHKJF_004701"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP0951935.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2318..3949)
                     /locus_tag="ACHKJF_004702"
     CDS             complement(2318..3949)
                     /locus_tag="ACHKJF_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP0951936.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRSGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4169..7852)
                     /gene="metH"
                     /locus_tag="ACHKJF_004703"
     CDS             complement(4169..7852)
                     /gene="metH"
                     /locus_tag="ACHKJF_004703"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP0951937.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLSIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFPNYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            8052..8876
                     /gene="iclR"
                     /locus_tag="ACHKJF_004704"
     CDS             8052..8876
                     /gene="iclR"
                     /locus_tag="ACHKJF_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP0951938.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2090 a         2502 c         2334 g         2136 t
ORIGIN      
        1 aaacgaattc ccgtacactg aactctctgg ctcactgagg gcaccatcat ggcactcccc
       61 cgtattaccc aaaaagagat gactgaacgc aagcagcgtg aacttaaaac actgctggat
      121 cgggcgcgga ttgcgcatgg tcgcgtactg accaactccg aaaccaacag cattaagaaa
      181 gagtacatcg ataaattaat ggtcgagcgt gaggctgaag cgaaaaaagc ccgccaattg
      241 aagaaaaagc aggcttataa accggatcct gaagcgtcat ttagctggtc agcaaatacg
      301 tcgacgcgcg gcaggcgcta atgaacaatc aacgcctgag attttcactc gtctgtgtgg
      361 acacaatctt cccctcaccc taaccctctc cccgaagggg cgaggggact gtccgagctc
      421 tttttggacg ttttcatcag tctgaggcgt taatcagcgt cccttctttt tacgccccgg
      481 cgaggtaaaa cgcttaccgt tggacgccgg gcgaccgcct ttttccgccg ttttttccat
      541 cttaacgact ggacgtttga tgcccgctgt tttcggtttg gccttcgctt taggttttgc
      601 ctcggaagag gaattttcga tgagcttaaa gaggtcgatt aactcatcat cagttaaatc
      661 gcgccactca cccagcggaa tgccgcttaa actgacgttc ataatgcgcg tacgttccag
      721 ctttttcact tcgtaaccaa agtgctcgca catgcgacgg atctgacggt tcagcccctg
      781 caccagggta atgcggaaga caaacggcgc ttcttttttg actttacact ttttagtcac
      841 tgtcccgagg attggcaccc ccgcccccat accacgaata aattcatcgg taatcggttt
      901 atcgaccgta accagatact ctttctcatg atcgttaccc gcacggagga tcttattcac
      961 cagatcgccg tgattggtga ggaaaatcag cccctgggag tctttatcca ggcggccaat
     1021 cgggaacacg cgtttgctgt ggttaacgaa atcgacaatg ttatcgcgct cgccatcttc
     1081 ggtggtgctg acaataccga cgggcttgtt cagggcgata agtaccaaat cttcggcttc
     1141 ccgaggctca atcaactgac cgtttacttt cacaacgtcg ccgggtttca cctgatcgcc
     1201 aatggcggct cgcttgccat taaggaacac attgccttgc tcgatatagc gatccgcttc
     1261 gcggcgtgag caaattccgc tttcgctgat gtatttattt aaacggactg atgagtcggg
     1321 cagcataaat tctcctgtaa aaaacggaat ataccgtacc tttgggttga taaaaaatag
     1381 tcatgagcga actatttgtt atttccattg gcttgaattc gttacctgct caaatcactt
     1441 tccagaagca atgtgcgaca tcgtgagcca tgtcagtaag tggttaaatt cgcagtggta
     1501 gcatgccttc cgtgatttct tactaaccgg agaagtaatg gaactgcttt tattgagtaa
     1561 ctcgacgctg ccgggtaaag cctggctgga acatgcactg ccgctaattg ctgaacagtt
     1621 gcagggtcgc cgctcagcgg tgtttatccc tttcgctggc gtaacgcaga cctgggatga
     1681 ttacacagcg aaaacggctg cggttctcgc tccgctgggt gtttctgtca ccggtattca
     1741 tagcgttgtc gatcccgttg ccgcgattga aaatgctgag atcgtgattg tcgggggcgg
     1801 gaatactttc cagttgctga aacagtgccg cgagcgcggg ctgctggcac caattactga
     1861 cgtggttaaa cgtggtgcgc tgtatattgg ctggagcgca ggcgctaacc ttgcttgccc
     1921 aaccattcgt accaccaacg atatgccgat tgtcgatccg caaggtttcg atgcgctaaa
     1981 tctgttcccg ctgcaaatca acccgcactt caccaacgcg ctgccggaag gccataaagg
     2041 tgaaacccgt gagcagcgta ttcgcgaact gctggtcgtc gcgccagaac tgacgattat
     2101 tggtctaccg gaaggtaact ggatcacagt gagtaaaggt cacgctacgc tgggtggccc
     2161 gaacaccact tatgtgttta aggctggtga agaagcggtg ccgctagaag ctggtcaccg
     2221 tttttaagtg aattgccgga tgcatgatcc ggcatcttgt tcatagtgcc tgatgcgacg
     2281 cttgacgcgt cttatcaggc ctacaggatt cacaatatta atactcgtcc cgtccttcat
     2341 cttcatccgg ctgttccagc acactgtaag ccaccgaaca aaacagcgag ttcaggcgct
     2401 gcatatctcc cagtaagcct aaatgtagcg aactggtttc aatgctctgc acgttttgct
     2461 gatgcaggcg atcgacgtgg gcgtgggaat agcggcgatt aagaatgcga aaacgatgtt
     2521 tgctgcgacg caaacgacga gcgctggtga catcgccaga gaagaacacc gacattgcca
     2581 gttttaaatt actgagcaat tgctcataga gcgcatccag ttccttcaag ccatcaagcg
     2641 aaaatgcccg ccgtgctgcc agcgatttat cggcaatttc gctgcccatg cgctcgacga
     2701 tatcggaggc ctgttcaagg ttgagcgaca tttcgatgat ctccgcccag cggcgcgact
     2761 cttcctccgc caactcttct tttggcatcc gcgccagata cagcttaatg gcggtgtaga
     2821 gaacgttgat atcatctgcc agcttacgca gctctttctc ctgccgtggc tcgccgtgca
     2881 tcactttatt tagcccttcc atcatctgtt ccatggcgtc gccaatgcgc agggtttcgc
     2941 gcgcggcgtt cgccagagca agcgtgggcg tatccagcgc gctgacatcc agatgtttgg
     3001 gccgtagctg ggtatccagt tccggttcat cgcgaataat cgttttgcaa aaccgtgcca
     3061 tcgggtcaac aaatggcagc atgaccaggc aacgtacaag gttgtagaag acgtggaaat
     3121 agatcaccag ttccgctttt ggcaatgaca acttccccat tgtctctgcc agcaaatgga
     3181 caaacggcag gataatcagg ctacccacca gcttaaacag cagactaccc agcgcgacac
     3241 ggcgggctgc ggcattggcg gcactgttgt tgagcatcgc cagcagaccg gaaccgaggt
     3301 tagcaccaat caccagacag agcgccacgg ggaaggagat aatgcctgcg gcggtcagag
     3361 tcgcggtcag cagtacagca gcaaggctgg agtagctgat aatggcgaac atcgcgccaa
     3421 tcagcgcatc cagcagaata tcgccggtca gcgaggcaaa gatcacctga acgccgtttg
     3481 cctgggtgat cggcgttacg gcctgcacaa tcaactccag cgccagcaaa atcagcccaa
     3541 gaccaatacc gacgcggccc agttgcccgg agcgtgactg tttgcgtccg aggaaaaaaa
     3601 tcacgccgat aaaaataagt aacggtgaca gccaggataa gtcgaaggtg agaatacgcg
     3661 ccattagcgc cgtcccgaca tctgcaccca gcacaatgac cagagccggt gcgagggcta
     3721 ccagatcctg agcgacaaac gaggtcacca gcatggtggt ggcattactg ctctgtacca
     3781 gtgcggtaac gccgatcccc gcgcaaaagg cgagcggctt tttttcgacg ctccggctaa
     3841 ggacagtacg caaacgcgcg ccgaagacgc gcattacgcc ggttcgaaca atatgagtcc
     3901 cccagaccag cagggcgacg gcagaaagca ggtgaagcag cgttaacacg agttcaggtt
     3961 ctccttatcg ttatatgttc ctgaagcaat tgaatgacca aaggaccgga aacacgctga
     4021 ctggcatctt tactgtgcct tttgaccttc gtttttccgg ctaagattat tccagcctga
     4081 ttccagtata agtgtttcat attaataaag agacagggca ctcattgagc gccctgtttg
     4141 ttgtaaggaa aagtgacaga tttgtgaatc agtccgcgtc ataccccaga ttcggtgcca
     4201 gccagcgctc aacttcggta acgctcatac ctttacggcg ggcatagtct tcaacctggt
     4261 cgcgctgaat ttgcgctacc gcataatact tgctgtccgg gtggctgaag taccaacccg
     4321 aaaccgatgc accaggccac atggcaaaag attctgtgag tttcatgcca gtgtgttttt
     4381 ccacttccag cagctcccag atggtggctt tttccgtatg ttccgggcag gccgggtagc
     4441 ccggtgccgg acggatgccc tggtagtttt cgcggatcag ttcttcgttg ctgaggttct
     4501 cgttcggcgc atagccccag tagactttac gcacacgctc atggagatac tccgcaaagg
     4561 cttccgccag acggtcggca agcgctttca ccatgatttt gttgtaatca tcgtgctgcg
     4621 cttcaaaggc atcagccagt gcgtcctctt ccagcccgcc agtcacggca aatgcgccga
     4681 tgtaatctgc tttaccagaa agcttcggcg caacgaagtc agcgagacag tagttaggga
     4741 atcccgtttt ttcggtctgt tgacgcagat ggtggctgac gttgatcaca tgggtgcgcg
     4801 tttcgtcacg gtagatttca atgtcatcgc ccacacggtt tgccgggaac aggcccacca
     4861 cgccacgcgg attcagagtt ttctcggcgc ttaatttatc cagcatgtcg ttggcgtctt
     4921 taaacagccg ctgcgcctca acgcccacca cttcatcttc cagaatgcgc ggatacttcc
     4981 cggccagcga ccaggtcata aagaacggtg tccagtcgat gtaattacgc agcgtttcga
     5041 tgctggcttc aacttcctgc acgccgagac ggtgcgccac cggcggcgtg taagcctgcc
     5101 agtcaaaagc gaaatcgtta tcgcgcgccg cttccagcgt gaccggtggt gtgcgcggtt
     5161 tcttacgccc gtgctgaata cgtacggttt cgtactcctt gcgggtacga gcgacaaaat
     5221 catcacgctg agtatcggaa agcagcgccg ccaccacacc aacggtgcgc gatgcgttct
     5281 gtacatacac cgtcgggccg ctgtagttct gctcaatttt caccgccgtg tgcgcttttg
     5341 aggtcgtcgc gccgccaatc aatagcggga tggtgaagcc ctgacgctcc atctctttcg
     5401 ccacgttaac catctcgtcc agcgacggcg tgataagccc cgaaagtcca atcagatcag
     5461 cattcacttc tttagcggta cggagaattt tttccgcagg caccataacg ccgagatcga
     5521 caatttcgta gttgttacat tgcagcacca caccaacgat atttttaccg atgtcgtgga
     5581 cgtcgccctt cacggtggcg atcaccatct tgccgttggt tttgccctgc tctttgctgg
     5641 cttcaataaa cggttcgaga tacgccaccg cctgtttcat gacgcgcgcc gatttgacca
     5701 cctgtggcag gaacattttc ccttcgccaa acagatcgcc gaccacgttc atgccgtcca
     5761 tcaacgggcc ttcaatcact tcaatcgggc gcgtagcctg ctggcgggct tcttcggtat
     5821 cctgctcgat aaactcggta atgcctttga ccagcgagta ttccagacgt ttattcactt
     5881 cccacgagcg ccactccgcc tgctgggcgt tagcggtgtc gtcggttttg ctgccgcgat
     5941 atttctcggc aagctccagt aaacgctcag tgccatcatc gcgacgatta agaatcacat
     6001 cttccaccgc gtcgcgcagt tcagcgggca ggtcgtcgta aatcgccagc tgcccggcat
     6061 tgacgatccc catatccatg ccattgcgaa tagcgtagta gaggaacact gcgtgaatgg
     6121 cttcgcgcac cggatcgttg ccacggaacg agaaagaaac gttagatacg ccgccggaaa
     6181 ttagcgcgtg cggcagttcg cgtttgatgt cttcacacgc gccgataaag tcctgcgcgt
     6241 agttgttgtg ctcttcaatg ccggtggcaa ccgcgaagat gttcgggtcg aagatgatat
     6301 cttctggcgg gaagccaacc tcttcggtga ggattttgta cgcccgacgg caaatctcga
     6361 ttttccgtgc gcgggtatcg gcctgtccct gttcgtcaaa ggccattacc accactgccg
     6421 caccgtagcg acgcaacaat ttcgcgtgat ggataaaggc gtcgacgccc tctttcatcg
     6481 agatagagtt aacaatgcct ttgccctgga tacacttcag acctttttca atgacatccc
     6541 attttgagga gtcgatcata atcggcacac gagcgatatc cggttcaccg gcaatcagat
     6601 tgagaaaacg caccatcgcc gcttcggcat cgagcatccc ttcatccatg ttgatatcga
     6661 taatctgcgc gccgttttcc acctgctggc gggccacatc cagtgcctcg ctgtatttct
     6721 cttctttgat caggcgcttg aacttagcgg aaccggtgac gttggtgcgt tcacccacgt
     6781 tcacaaacag gctatcttcg ccaatgctca gcggctccag gccggacaaa cggcaggcta
     6841 cggggatttc cggcagtttg cgaggcgcta atccttctac tgcacgactc atcgctgcaa
     6901 tatgttgtgg cgtggtgcca cagcagccgc cgacgatatt gagaaaaccc gcttgcgccc
     6961 attcacgtat ctgttttgcc atcgtgtcgg cgtcgagatc gtactcacca aaggcgttgg
     7021 gtagcccggc gttcgggtgc gcggtgacgt agcattccgc aatccgtgac agctcctgca
     7081 cgtactggcg cagttcatcg ggccccagcg cacagttcag gccaaaggtc agagcttcgg
     7141 cgtggcgcaa tgagttgtaa aatgcttcgg tggtctgccc ggagagcgtg cgcccggagg
     7201 cgtcggtgat ggtgccggag atcataatcg gcagctcaac gcccagcgct tcaaactccg
     7261 ttttcaccgc aaaaaccgcc gctttggcgt taagggtgtc gaaaacggtt tcaatcagga
     7321 tcagatccgc accaccttcc accagcgctt tggtggattc gcgataggcc gccaccagct
     7381 ggtcaaaagt gatgttgcgg aatgccggat cgttgacgtc cggagaaata gacgccgtgc
     7441 ggttggttgg gccgagaaca ccggcaacgt agcgcggttt ctccggcgtg cgcgcggtcc
     7501 actcgtcagc acaggcgcgc gccagtttcg ccgccgcaaa gttgatttcc gccgacaggg
     7561 attccatctg gtaatccgcc atcgcaatgg tcgtggagtt gaaggtgttg gtttcgatga
     7621 tatccgcgcc cgcttcaaag taggcgttgt ggatagcggc gatcacttcc ggtttgctga
     7681 gcaccagcag gtcgttgttg cctttgagat cgcatggcca gtcggcaaag cgttcaccac
     7741 gaaaatcggc ttcgttcagt cgatagctct ggatcatggt gcccataccg ccgtccagca
     7801 ccagaatacg ttcatttaac tgcgcacgca gttgttccac tttgctgctc acacttgctc
     7861 ccgacacgct caacccagat ttaataaaaa ttcaacaaac catactggca taaacgcatc
     7921 tgtggtaaaa gcgaccacca cgcaacatga gatttgttca acattaactc atcggatcag
     7981 ttcagcaact attgcattag ctaacaataa aaatgaaaat gatttccacg atacagaaaa
     8041 aggaggctgt catggtcgca cccattcccg cgaaacgcgg cagaaaaccc gccgttgcca
     8101 ccgcaccagc gactggacag gttcagtctt taacgcgtgg cctgaaatta ctggagtgga
     8161 ttgccgaatc caatggcagt gtggcactca cagagctggc gcaacaagcc gggttaccca
     8221 attccacgac ccaccgcctg ctaaccacga tgcaacagca gggtttcgtg cgtcaggtcg
     8281 gcgaactggg acattgggca atcggcgcac atgcctttat ggtcggcagc agctttctcc
     8341 agagccgtaa tttgttagcg attgttcacc ctatcctgcg caatttaatg gaagagtctg
     8401 gcgaaacggt caatatggcg gtgcttgatc aaagcgatca cgaagcgatt attatcgacc
     8461 aggtacagtg tacgcatctg atgcgaatgt ccgcgcctat cggcggtaaa ttgccgatgc
     8521 acgcttccgg tgcgggtaaa gcctttttag cccaactgag cgaagaacag gtgacgaagc
     8581 tgctgcaccg caaagggtta catgcctata cccacgcaac gctggtgtct cctgtgcatt
     8641 taaaagaaga tctcgcccaa acgcgcaaac ggggttattc atttgacgat gaggaacatg
     8701 cactggggct acgttgcctt gcagcgtgta ttttcgatga gcaccgcgaa ccgtttgccg
     8761 caatttcaat ttccggaccg atttcacgca ttaccgatga ccgcgtgacc gagtttggcg
     8821 cgatggtgat taaagcggcg aaggaagtga cgctggcgta cggtggaatg cgctgacttt
     8881 ttctggcggg cagaggcaat attctgccca tcatacctga gtggcaatag aataagggtg
     8941 tctgttaatc gcattgacgc caaaataact taatgtcata cacttcacac acttttatta
     9001 actcagcatt atttttaaac atcaaaccac ttaatcatta acaaaatcgc aagatctcat
     9061 tc
//
LOCUS       DAZGXZ010000181         8932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000181 DAZGXZ010000000
VERSION     DAZGXZ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.181"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1149
                     /locus_tag="ACHKJF_004705"
     CDS             <1..1149
                     /locus_tag="ACHKJF_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0951939.1"
                     /translation="MKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQ
                     TKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLA
                     HNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPL
                     IALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKL
                     AVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLV
                     SIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYD
                     VPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1330..2820
                     /gene="dgcJ"
                     /locus_tag="ACHKJF_004706"
     CDS             1330..2820
                     /gene="dgcJ"
                     /locus_tag="ACHKJF_004706"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP0951940.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2863..3366
                     /gene="yeaK"
                     /locus_tag="ACHKJF_004707"
     CDS             2863..3366
                     /gene="yeaK"
                     /locus_tag="ACHKJF_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP0951941.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3367..3471)
                     /gene="yoaI"
                     /locus_tag="ACHKJF_004708"
     CDS             complement(3367..3471)
                     /gene="yoaI"
                     /locus_tag="ACHKJF_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP0951942.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3641..4087
                     /gene="yeaL"
                     /locus_tag="ACHKJF_004709"
     CDS             3641..4087
                     /gene="yeaL"
                     /locus_tag="ACHKJF_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP0951943.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4044..4865)
                     /gene="nimR"
                     /locus_tag="ACHKJF_004710"
     CDS             complement(4044..4865)
                     /gene="nimR"
                     /locus_tag="ACHKJF_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP0951944.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4962..6143
                     /gene="nimT"
                     /locus_tag="ACHKJF_004711"
     CDS             4962..6143
                     /gene="nimT"
                     /locus_tag="ACHKJF_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP0951945.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6198..6545
                     /gene="yeaO"
                     /locus_tag="ACHKJF_004712"
     CDS             6198..6545
                     /gene="yeaO"
                     /locus_tag="ACHKJF_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP0951946.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6567..6821)
                     /gene="yoaF"
                     /locus_tag="ACHKJF_004713"
     CDS             complement(6567..6821)
                     /gene="yoaF"
                     /locus_tag="ACHKJF_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP0951947.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRYHPAGEK"
     gene            7004..8029
                     /gene="dgcP"
                     /locus_tag="ACHKJF_004714"
     CDS             7004..8029
                     /gene="dgcP"
                     /locus_tag="ACHKJF_004714"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP0951948.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8062..8160
                     /gene="yoaK"
                     /locus_tag="ACHKJF_004715"
     CDS             8062..8160
                     /gene="yoaK"
                     /locus_tag="ACHKJF_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP0951949.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8163..8237
                     /gene="yoaJ"
                     /locus_tag="ACHKJF_004716"
     CDS             8163..8237
                     /gene="yoaJ"
                     /locus_tag="ACHKJF_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP0951950.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8296..8544)
                     /gene="yeaQ"
                     /locus_tag="ACHKJF_004717"
     CDS             complement(8296..8544)
                     /gene="yeaQ"
                     /locus_tag="ACHKJF_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0951951.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8772..>8932)
                     /locus_tag="ACHKJF_004718"
     CDS             complement(8772..>8932)
                     /locus_tag="ACHKJF_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0951952.1"
                     /translation="FRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQEP
                     TEMIQATA"
BASE COUNT         2221 a         2149 c         2090 g         2472 t
ORIGIN      
        1 atgaaatatt tcactaacaa acaaatttta ccaacactca ttttaagcct tgcattttta
       61 agtggcctta tctatttagt tgaaaccatt gtaattatcc ataaaccaat taacggcagt
      121 acactgatcc agacaaagtc gaatgatgtt tctattttct atattttccg ccaactcagt
      181 tttatttgtt taacctcgct ggcactcttt tgttatggaa aagacaacat ccttgacaac
      241 aataagaaaa aaacgggaat cctgttgctg gcgctgattc cttttttagt ttttcccctt
      301 ctggcacaca atctgagcag ttataacgct gactattctt tgtatgtcgt cgactactgt
      361 ccggacaacc atactgcgac ctggggaatc aactatacaa aaatattggt ttgtctgtgg
      421 gcatttttac tgttctttat tatcatgcgt acacgattag ccagcgaact atggccgtta
      481 atagcattat tatgtctggc atcgctatgc tgcaacttac ttctactgac tctggatgag
      541 tataattaca ccatctggta tatcagtcgc gggattgaag tttccagtaa actgtttgtt
      601 gtgtcttttc tgatttataa catttttcag gaactgcaac tctccagcaa actggcagtt
      661 catgatgtgc tgaccaatat ttataatcgg cgctactttt tcaacagcgt agagtcatta
      721 ttgtcgcgac ctgttgttaa ggacttctgt gtcatgctgg ttgatattaa tcagttcaaa
      781 cgcatcaatg cccaatgggg acatcgtgtg ggtgataaag tgctggtttc aattgtcgat
      841 attatccagc aaagcatccg ccctgatgat attttagcgc gactggaggg tgaggtgttt
      901 ggcttgctat ttaccgaact caatagtgcc caggcaaaaa tcattgcgga acgtatgcgt
      961 aaaaatgtcg aactcctgac cggctttagt aacagatatg atgttcctga acaaatgacc
     1021 atcagtattg gcacggtttt ttcaacgggt gacacgcgta atatctcgct tgtcatgacg
     1081 gaagcagata aagccttacg cgaagcgaaa agcgaggggg gcaacaaagt gattattcat
     1141 catatttaag tgcaaaaata ttcagagcca tgctttttgc gtggcttttg catacaattt
     1201 attactatta cccctaaatt tcccctcccc agcggcgcag agatgagtat aattagcgcc
     1261 cctgtgccag gccgcaatcg aactttatct ggttttctcg tttcactaac cgaaggagtg
     1321 ccatttatca tgaaattgca ccatagaatg ctccggcatt ttatcgccgc aagtgtcatt
     1381 gtgctgacat cttccttcct tatttttgaa cttgtcgcca gcgacagagc aatgagtgcc
     1441 tatctgcgct atatcgtgca gaaagcagac tcctcctttc tttatgataa gtatcagaat
     1501 cagagtattg ccgcgcatgt gatgcgcgct ctcgctgctg agcagtcgga agtgtcgcca
     1561 gaacagcggc gcgccatctg cgaggctttt gagtctgcca ataataccca tggcttaaac
     1621 ctgactgccc ataaataccc gggcttacgc ggcacactac aaaccgcatc cactgactgc
     1681 gacacaattg tggaagctgc agcactatta cccgcttttg atcaggcagt ggaaggcaac
     1741 cgccaccagg atgattacgg ttcaggtctt gggatggccg aagagaaatt tcactattat
     1801 ctcgatctca atgaccgcta tgtctatttt tatgagccgg ttaatgttga atactttgcg
     1861 atgaataact ggtccttcct gcagtcagga agtattggta tcgatcgcaa agatattgaa
     1921 aaggtattta ccgggcgtac cgtattgtcg agcatttacc aggatcagcg tactaaacag
     1981 aacgtgatga gtttgctgac gccggtgtat gttgcagggc agctaaaagg gattgtgctg
     2041 ctggatatta acaaaaacaa tttgcggaat atcttttaca ctcatgaccg ccctctcctc
     2101 tggcgttttc tcaatgtcac gctaaccgat accgattcgg ggcgcgacat tatcatcaac
     2161 cagagcgaag ataatctgtt ccagtatgtc agttacgtcc atgacttacc gggcggcatt
     2221 cgtgtctcgt tatccattga tattctttac tttatcacgt cttcgtggaa aagcgttctg
     2281 ttctggattt tgacggcgtt aattttgctg aatatggtcc ggatgcactt ccgtttatac
     2341 caaaatgtgt cgcgagaaaa tattagtgat gcgatgactg gactgtataa tcgcaaaatt
     2401 ttaacccctg aactggagca gcggttgcag aaactggtgc aatccggttc ttcggtgatg
     2461 tttattgcaa ttgacatgga caagttaaag caaataaatg acaccctcgg tcatcaggag
     2521 ggggatttag cgattacgtt attagctcag gcgattaaac aatcgattcg taaaagtgat
     2581 tatgccatcc gactcggtgg cgatgaattc tgcatcattc ttgtcgattc gacgccgcaa
     2641 attgcagcac aactgcctga acgtatcgaa aaacgtctgc aacatatcgc gccgcagaaa
     2701 gaaatcggct tctcttccgg tatttacgcg atgaaagaaa acgatacgtt acatgatgcg
     2761 tataaagctt ccgatgagcg tttatatgtc aataagcaga acaaaaacag ccgttcatga
     2821 taaccttctg tggttgtttg cttgtaatct caggagcgtg aaatgactga aatggctaaa
     2881 ggaagcgtga cgcatcagcg gttaatcgct ttattatcac aagaaggtgc taacttccgc
     2941 gtcgttaccc atgaagcggt aggtaaatgt gaagcggtgt cagaaattcg tggcaccgct
     3001 ctaggccagg gtgctaaggc actggtatgt aaagtcaaag gcaatggcgt aaatcaacat
     3061 gttctggcga tcctcgctgc cgatcaacag gccgatctga gccaactcgc cagccatata
     3121 ggcggattac gcgcttcgtt ggccagcccg gcggaagtcg atgaactcac aggctgtgtc
     3181 ttcggcgcga tccccccttt cagcttccat ccaaaactca aactggttgc cgaccctcta
     3241 ctcttcgaac gatttgatga aatcgccttc aatgcaggga tgctggataa atccgttatt
     3301 ttgaaaacca cggattattt gcgcattgcg caaccagaac tgctcaattt ccgccgcact
     3361 gcgtaactag cctgtccgtt caataagcag aacggacaaa accagcacaa cagcaatggc
     3421 aaaaaacgat gacgtgataa tcagtgtctc gacgaacatt tgatcgttca tagcatgccc
     3481 ccgggagata gctgttttca cgttattgtt agcgtgcaca aatggcagtt tgatgacagt
     3541 tcgcctgatt ttttatgcaa aaaacgtaaa gattttctac ttccttcctg cagcaagcgt
     3601 aaagtaagca ggcttattat tttttggcaa ggaaaccacg atgtttgatg tcactttgct
     3661 gatcctgctc gggttagctg cgctgggctt tatcagtcat aacaccactg tcgccgtttc
     3721 aattctggtg ttgatcattg tccgcgtcac accgttaagc acctttttcc cgtggattga
     3781 aaaacagggg ctaagtatcg ggataatcat tctgacgatt ggcgttatgg cccccatcgc
     3841 cagcgggacg ctaccgccct caaccttgat tcactcgttt ctgaactgga aatcgctggt
     3901 ggcaattgca gtaggcgtta ttgtctcctg gctgggtggg cgcggcgtga ccttaatggg
     3961 cagccagccg caactggtcg ccgggctgct ggtaggcact gttttaggcg tggcgctgtt
     4021 tcgcggcgta ccggtcggcc cgcttattgc cgcgggtctg gtttcgctga ttgtggggaa
     4081 acagtagtta atcccgcaat atatcggcct ggcgtttgcc ccaggccttt cttaaacata
     4141 gtaataaagg ccgttgttga atcatacccc agcgtatgag caactttctg tacagtatcg
     4201 cctttcacta atccctgaag tgccataatc agctgtaact gctgacgcca ctggcgaaaa
     4261 ctcaaccccg tctcttttac tatcagtcgc gcaagattgc gttcactcat cgcaaaaaag
     4321 ccagcccatt gccctaatga cccccactcg acgggccctt tcgccatcat ctccaccatc
     4381 gtgcggattt taggatgaga agaaacgggc aggtgcagct gttgttgtgg ctgctgaggg
     4441 agttcatcaa acaggacctg aattaatctg cgggtcatcg gctcagcacg ctgagtcgtc
     4501 gtcctgtttg ccagggttaa aatcaattcc cggcataagg gcgatatttt caatgtgcaa
     4561 catgttgtcg gcattgtcac tgcgctgggt tcgataaaca gaaaacagag ttcagcgtta
     4621 gctgtcactt gattgctatg ttccaccccg cccggtatcc aaaccgcata ttgcggcggc
     4681 accatccaca aagcattttc caccgtacag gtaattgcac catgtaatgc tagtatcagc
     4741 tgtcctttgc ggtgctgatg tacaggactg gttaactcat ccgttccggc gtgaatacaa
     4801 aatgcgaccg cggcttcatg gtgtcggtcg ggttcatagc cattgagatt caacctgtgc
     4861 atcattttgt ccgaatttag cgataatttg tcattttagc ttgattcaac ataacaataa
     4921 aaacggtaag gtacagcctc gtttgtaaca atgagaagca tatgacctgt tcaacttcat
     4981 taagcggcaa aaacaggatt gtccttatcg ctggcattct gatgattgcc actacattac
     5041 gcgtcacctt caccggcgca gcaccgttac tcgatacgat tcgttccgct tactcgctga
     5101 cgacagcgca aaccggctta ttaaccaccc tgccattatt ggcctttgcg ctaatctcac
     5161 ctttggctgc cccggtggcg cgacgttttg gtatggaacg tagcctgttt gccgcgttac
     5221 ttttgatctg tgcaggtatc gcaattcgtt ctctcccttc gccttactta ttatttggcg
     5281 gtacagcggt cattggcggt gggattgcat taggcaatgt cttactgcca ggattaatta
     5341 aacgcgattt ccctcattcc gtcgccagac ttaccggcgc atattccctg acaatgggag
     5401 ctgcagcggc actgggatcg gctatggtcg tgccgctggc tttgaacggt tttggctggc
     5461 aaggcgcgtt gttcatgctg atgtgttttc ctctgctggc tcttttttta tggctgccac
     5521 agtggcgaag ccaacaacat gcaaatttga gtacctcgcg cgccttacat actcggggta
     5581 tctggcgttc gccgcttgcc tggcaggtca cattgtttct tgggatcaac tcactggtct
     5641 attacgtgat tattggctgg cttccggcga tcctcatcag tcgcggctat agcgaagcac
     5701 aggcgggttc actgcatggt ttgctgcaac tagccacagc agctcccggt ttgctgatcc
     5761 ctcttttctt acatcatgtg aaagatcagc gtggtattgc agcgttcgtt gccttgatgt
     5821 gcgcagtggg cgctgttggg ctctgcttta tgccagcgca cgcgatcacc tggactctgc
     5881 ttttcggttt tggttccggc gcaacaatga tactggggtt gacgttcatt ggtcttcggg
     5941 caagttctgc gcatcaggcg gcggcactct cggggatggc acaatccgtc gggtatttgc
     6001 tggcagcctg tgggccgccg ctgatgggta aaatacacga tgctaacggt gactggtccg
     6061 taccactgat gggtgttgcc atactttcac tactgatggc gattttcgga ctttgcgccg
     6121 ggagagacaa agaaattcgc taatatccgg tgctatagtg acgtgacaaa tcatgcgtga
     6181 aagggagaac aaacacgatg aatattcagt gcaaacgcgt ttatgatccg gctgaacaga
     6241 gcgatggtta tcgcgtactg gtcgaccgcc tctggccgcg cggtatcaaa aaaaccgatt
     6301 tagcccttga tgagtgggat aaagaaatca cgccgtcaac ggaactgcgc aaagcctttc
     6361 acggcgaagt cgtcgatttt gcaacctttc gcgagcaata tcttgcagaa ctggcgcaac
     6421 acgagcaaga aggaaagcgg ctggcggaca tcgccaaaaa acagcccctg accctgctct
     6481 acgcagcaaa aaacaccacg cagaaccatg cgctggtgct ggccgactgg ctacgtagct
     6541 tgtgatttta gtacagcatc cggcggttat ttttcaccag ccggatggta ccgtcgccac
     6601 aatgcccatt catcaatcgt ttcaccgccc ggtaatttgc aattgttgct gaccccttgc
     6661 gctgtctgca ctggaatgag cgtcccgccc ttctgctggc aatagaccga cgccggattt
     6721 gccataccaa tctgcggcgg tttaggtgct tctggctgag aaggggttga acaaccagcc
     6781 aggaccagca agcaaggcag aacaaaactg ataattttca tttattgatc tcacatattt
     6841 atccagactt agagtatcgc ggtatcgttt tgttttgcag cactattttt attacattca
     6901 ctcaaatcat attacgcctt gtttcatctt tgttgatgat gttttatcat gcctgcaaag
     6961 attaaataat cagcatttac ccgccgtatc ctggagttgt ttcgtgtcag atcagattat
     7021 cgcccgcgtc tcgcaatccc ttgccaaaga acagtcacta gaaagtctgg tccgacagct
     7081 tctggagatg ctggaaatgg tcactgatat ggaatcaacc tacctgacca aagtggatgt
     7141 cgaagcgcgc ctgcagcata ttatgtttgc ccgtaacagc cagaaaatgc acatcccgga
     7201 gaattttacc gtctcgtggg attactcgtt atgcaaacgc gctattgatg aaaactgctt
     7261 tttcagcgat gaagtccctg accgttgggg cgactgtatt gcggcacgca atcttggaat
     7321 caccacattt ctgagtacac caattcactt accggatgga tcattctatg gcacgctttg
     7381 cgccgccagc agtgagaagc gccagtggag tgaacgcgcg gaacaggttt tacagttatt
     7441 cgccggactg attgcacaat atattcaaaa agaggcgttg gttgaacagc tgcgcgaagc
     7501 caatgccgcg ctgattgcgc aatcgtatac cgactcgtta accgggctac cgaatcggcg
     7561 ggcgattttt gaaaatctga cgacgctgtt ttctctcgcc cggcatctta accataagat
     7621 aatgatcgcg tttatcgatc tggataactt caaattaatc aatgatcgtt ttggtcataa
     7681 tagtggcgat ctgtttctca ttcaggttgg cgagcgcctt aatacgctcc agcaaaatgg
     7741 cgaagttatt ggtcgtctcg gcggtgatga gtttttggtt gtttcactaa ataacgagaa
     7801 tgcggatatt tcgtcgctgc gagaacgtat tcaacagcaa atacgtggag aatatcactt
     7861 aggtgatgtt gatttgtatt atcccggtgc cagtcttggc atagtagaag tcgatcctga
     7921 aacaaccgat gcagacagtg ccctgcatgc tgccgatatt gcgatgtatc aggagaaaaa
     7981 acacaaacag aaaacacctt ttgtcgcgca tccagcgcta cattcctgag gcgtattcac
     8041 atccttttga ttggtgataa catgcgaatc ggtattattt ttccggttgt aatctttatt
     8101 acagcggtcg tatttttagc atggtttttt attggcggct atgctgcccc gggagcataa
     8161 agatgaaaaa aacaacgatt attatgatgg gtgtggcgat tattgtcgta ctcggcactg
     8221 agctgggatg gtggtaacgt cacctctaaa aaatagcaaa ggctgcctgt gtgcagcctt
     8281 tgtgcaattt aagcgttaac ttttaatctt cctgtagata aatagcacga caatcgcacc
     8341 aataacggca accacgaagc tgccaaaatt gaagccatcg actttaccaa agccaaacag
     8401 cgtgctgatc catccgccaa ctacggcacc gactatcccc agcaggatag tcataaagaa
     8461 tccaccacca tctttacctg gcatgatcca cttcgccaga ataccggcaa taagcccaaa
     8521 aataatccat gacagaatgc ccattgtttc ctcacttatc tgttttgcat tagcgggtta
     8581 gtcgctgata aaaagcatag cacaacatcg ggagggcaag atttgtgacg agcatcacgg
     8641 aggatttttt tgcggatggc acagaaattg cgccatcaac gatcagtgat aattaccaac
     8701 cacaaacatc atgttcgttt tccgtgtctt gacatcctcc acgccctgaa tgacgaggat
     8761 ccctgctacg tttaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg
     8821 cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga
     8881 gcgccgttta catcggcgtt cgctgtatat ccacatacct ggcatctgaa tt
//
LOCUS       DAZGXZ010000182         8903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000182 DAZGXZ010000000
VERSION     DAZGXZ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.182"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..4444
                     /gene="traI"
                     /locus_tag="ACHKJF_004719"
     CDS             <1..4444
                     /gene="traI"
                     /locus_tag="ACHKJF_004719"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFP0951953.1"
                     /translation="WMLTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAV
                     TQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKG
                     LFTSGIHVLDELSVRALSRDIMKQNRVTIHPEKSVPRTAGYSDAVSVLAQDRPSLAIV
                     SGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQ
                     EGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKD
                     AGVNTYRWQGGEQRPATIISEADRNVRYARLAGDFVASVKAGEESVAQVSGVREQGIL
                     TRAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDR
                     YVIDRVTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPG
                     LRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGHSVSD
                     SAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKA
                     RAGETLLETAISLQKTGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTS
                     FTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLM
                     ERVPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESE
                     RPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLLQRSGETPNFSNTLFLLDESSM
                     VGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMNEIVRQ
                     TPELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEA
                     QQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAR
                     EKAGELGKEQVMVPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGL
                     ITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTV
                     TAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGN
                     RKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMN
                     AQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRS
                     AGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDSVRIAR
                     DNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAE
                     EAIRRETERRADEIVRKMAEEKPDQPDGKTEQAVRAIAGQEQDRASSSERETALPESV
                     LREPQRVLDAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            4493..4912
                     /locus_tag="ACHKJF_004720"
     CDS             4493..4912
                     /locus_tag="ACHKJF_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951954.1"
                     /translation="METYPDPDDIRKNTADILKALTVDNIPERHGFREELASLKNCIN
                     DDEYCYMTFYETGYAFLKALLRTRLRLKRTDPAHSLLPLISSSVEALRTQLKENEAYV
                     RLLIGMDAVSRWTGPLFCFAALMILILVGTVFAHVWF"
     gene            4967..5668
                     /gene="traX"
                     /locus_tag="ACHKJF_004721"
     CDS             4967..5668
                     /gene="traX"
                     /locus_tag="ACHKJF_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP0951955.1"
                     /translation="MKTREWPERLLVWSPGQRDIIKTVALVLMVLDHINLIFQLKQEW
                     MFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQFAYYLAGFPWYEGNIL
                     FAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAGLLMLAVSHRLYRAED
                     RAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSCAGKSLPRFWPGDFFP
                     VFYACHLAVLGVLAL"
     gene            5723..6283
                     /locus_tag="ACHKJF_004722"
     CDS             5723..6283
                     /locus_tag="ACHKJF_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP0951956.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPSLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKICRQNRIKAELQAVLDEK"
     gene            6415..6627
                     /locus_tag="ACHKJF_004723"
     CDS             6415..6627
                     /locus_tag="ACHKJF_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP0951957.1"
                     /translation="MNGFRNSSRNGQVWRWQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRSRGRV"
     gene            6873..7334
                     /locus_tag="ACHKJF_004724"
     CDS             6873..7334
                     /locus_tag="ACHKJF_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0951958.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            7380..7589
                     /gene="hha"
                     /locus_tag="ACHKJF_004725"
     CDS             7380..7589
                     /gene="hha"
                     /locus_tag="ACHKJF_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP0951959.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEIIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYNRIPKEIWKYVR"
     gene            7627..8217
                     /locus_tag="ACHKJF_004726"
     CDS             7627..8217
                     /locus_tag="ACHKJF_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFP0951960.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLKTTGADQQSPEHRS"
     gene            8457..8717
                     /gene="repA"
                     /locus_tag="ACHKJF_004727"
     CDS             8457..8717
                     /gene="repA"
                     /locus_tag="ACHKJF_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0951961.1"
                     /translation="MSQIENAVTSSPKRIYRKGNPLTGAEKQRISVSRKKGTHKAINV
                     FIQSELKDDLTQLCKDSGLTQKEMIEHWILKEKAAMTDANRK"
     gene            complement(8758..8889)
                     /locus_tag="ACHKJF_004728"
     CDS             complement(8758..8889)
                     /locus_tag="ACHKJF_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFP0951962.1"
                     /translation="MFFVAKHRKIIGVFAFLAPVNPHQNQFPATLRRGQPQNSLNDQ"
BASE COUNT         2145 a         2127 c         2774 g         1857 t
ORIGIN      
        1 atggatgctg acgctgaagg aaaccgggtt cgacatccgg gcatatcgtg acgcggcgga
       61 tcagcgagcg gagacccgca cgcaggcgcc cggggctgtt tcacaggagg ggccggatgt
      121 gcagcaggcg gtgacacagg ccattgccgg attaagtgaa cgaaaagtgc agttcacgta
      181 tacggacgtg ctggccagaa cggtcggtat actgccgccg gaaaatggtg tgattgaacg
      241 ggcacgcgcc ggtatcgatg aggccatcag ccgtgagcag cttatccccc tcgaccgtga
      301 gaaggggctg ttcacgtccg ggatccatgt gctcgatgag ctgtccgtcc gggcactcag
      361 tcgtgacatc atgaaacaga accgggtgac catacatccg gagaaaagtg tcccccggac
      421 ggccggttac agcgatgcgg tcagtgttct ggcacaggat cgtccgtcgc tggccattgt
      481 gtccgggcag ggcggtgcag ccgggcagcg tgagcgggtg gcggagctgg tcatgatggc
      541 ccgggagcag gggcgggagg tgcagattat cgctgctgac cgtcgctcgc agatgaacct
      601 gaagcaggat gaacggctgt ccggtgagct gataaccgga cgtcgtcagc tgcaggaggg
      661 catggccttc acgccgggca gtaccgttat cgttgaccag ggcgaaaaac tctccctgaa
      721 agagacgtta accctgctgg acggtgccgc acgtcataac gtacaggtcc tgataaccga
      781 cagcgggcag cgaaccggca caggcagtgc actgatggcc atgaaagatg ccggggtgaa
      841 cacatatcgc tggcagggcg gagaacagcg accggcaacc atcatcagtg aggcggaccg
      901 taatgtccgc tatgcccggc tggccggaga ttttgttgcc agcgtgaaag ccggagaaga
      961 gagtgtggca caggtcagcg gggtacggga acagggcatt ctgacacggg ccattcgcag
     1021 tgagctgaaa acgcagggcg tgctcggaca cccggaggtg accatgactg cactgtcacc
     1081 ggtctggctg gacagccgga gccgttatct gcgggatatg taccgtccgg ggatggtgat
     1141 ggagcagtgg aacccggaga cacgcagtca tgaccgctat gttatcgacc gggtgacggc
     1201 gcagagtaac agcctgaccc tgcgggatgc gcagggtgaa acgcaggtgg tgcgtatttc
     1261 ctccctggac agcagctggt cgctgttccg gccggaaaaa atgccggtgg cagacggcga
     1321 gcgactgagg gtgacaggga aaattcccgg actccgcgtc tccggcggtg accgcctgca
     1381 ggtggcatcc gtcagtgaag atgcgatgac ggttgttgtg ccggggcggg ctgaaccggc
     1441 ttccctgcct gtgagcgatt caccattcat ggcactgaag ctggagaacg gctgggtgga
     1501 aacgcctggg cattccgtca gcgacagtgc gaaggttttt gcctccgtca cacagatggc
     1561 aatggacaac gccaccctga acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc
     1621 ctcactggat gaaacccgta ctgcggaaaa acttgcccgc catccctcct ttacggtggt
     1681 ttctgagcag ataaaggcgc gtgccggtga gacattgctg gaaaccgcta tcagtctgca
     1741 gaaaaccggg cttcacacgc cggcacagca ggccattcat ctggcgcttc ctgtggtgga
     1801 aagtaaaaac ctggccttca gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc
     1861 agaaggaacc agttttactg aactgggagg ggaaatcaat gcgcagataa aacgcggtga
     1921 tttactgtat gtggatgtgg caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc
     1981 gtatgaggca gaaaagagca ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc
     2041 actgatggag agagtacctg gcgaactcat ggagaaactg acatcaggac agcgtgccgc
     2101 tacccgcatg atactggaaa cgtccgaccg tttcacggtg gtgcagggct atgccggtgt
     2161 gggtaagacc acacagttcc gggcggtgat gtcagccgtg aacatgctgc cggagagtga
     2221 acgtccccgt gtcgtggggc tggggcccac gcaccgtgcg gtcggtgaga tgcgcagcgc
     2281 cggcgtggat gcacagacgc tggcgtcctt tctgcatgac acgcagctgc tgcagcgcag
     2341 cggagaaacg ccgaatttca gcaacacgct gttcctgctc gatgagagct ctatggtggg
     2401 caataccgac atggcacggg catacgccct gattgcggcc ggtggcggtc gtgccgtggc
     2461 cagcggtgac acggaccagc tgcaggccat cgcgcccggt cagcctttcc gtctccagca
     2521 gacgcgcagt gctgccgatg tggccatcat gaatgagatt gtgcgtcaga cgccggaatt
     2581 gcgggaggcg gtatacagcc tgattaaccg ggatgtggaa agggcgctgt ccgggcttga
     2641 gagtgtgaaa ccgtctcagg tgccacgtca ggagggcgca tgggcaccgg agcactccgt
     2701 gacggagttc agtcacagcc aggaagcgaa actggcagaa gcgcagcaga aggcgatgct
     2761 gaaaggcgag gcttttcctg atgtccccat gacactgtat gaagccattg tccgcgacta
     2821 taccggcagg acaccggaag cacgggagca gacgctgatt gtcacgcacc tgaatgagga
     2881 ccggcgcgta ctgaacagca tgattcatga tgcacgggaa aaggccggtg agctggggaa
     2941 agagcaggtc atggtgcctg tcctgaacac agcgaatata cgtgacgggg agctgcgtcg
     3001 tctctccacc tgggagaata acccggatgc ccttgccctg gtggataatg tgtatcaccg
     3061 gattgccggt atcagcaagg atgacgggct gataaccctg caggatgctg aaggtaacac
     3121 gcggctgatt tcgccccggg aggcggtggc tgaaggcgtc acactgtaca ccccggacac
     3181 catccgggtg gggaccggtg accggatgcg cttcacgaag agtgaccggg agcgcggtta
     3241 tgtggccaac agcgtctgga cggtgacagc ggtttctggt gacagtgtca cactttctga
     3301 cggtcagcag acccgggtga ttcgccccgg tcaggagcgg gcagagcaac atattgacct
     3361 ggcctatgcc atcaccgccc acggtgcgca gggggcaagt gaaacctttg ccatcgcgct
     3421 tgaaggcacg gaaggtaacc ggaaactgat ggccggcttt gagtcagcct acgtggccct
     3481 gtcgcgtatg aagcagcatg tgcaggtgta caccgataac cgtcagggct ggacggatgc
     3541 cattaacaat gccgtacaga aaggtacggc ccacgatgtg tttgagccga aaccggaccg
     3601 ggaggtcatg aatgcgcagc ggctgttcag tacggcgcgg gagctgcggg acgtggcagc
     3661 tggtcgtgcc gttctccgtc aggcggggct ggccggggga gacagccctg cacggtttat
     3721 tgcaccggga cgtaaatatc cgcagccgta tgtggcactg ccggcgtttg accgtaacgg
     3781 caggtccgcc ggtatctggc tgaacccact gaccacggat gacggaaacg ggctgcgggg
     3841 attcagcggt gaaggacggg tgaaaggcag cggggatgcg cagttcgtgg ccctgcaggg
     3901 cagccgtaac ggagagagcc tgctggctga taatatgcag gatagtgtcc ggattgcccg
     3961 tgataatcct gacagcggtg tggtggtgag aattgccggt gaaggtcgcc cgtggaatcc
     4021 cggtgccatc accggcggtc gcgtgtgggg ggatatcccg gacaacagtg tccagccggg
     4081 tgccggaaac ggtgaaccgg tcacggcaga ggtgctggca caacggcagg ctgaagaggc
     4141 catccgccgt gaaacggaac gccgcgcaga tgaaattgtc cgtaaaatgg cagaggaaaa
     4201 acctgaccag ccggatggta aaacagagca ggctgtcagg gctattgccg ggcaggagca
     4261 ggacagggct tcgtcatcag aacgggaaac tgcgctgccg gagagtgtgc tgcgtgagcc
     4321 tcaacgggtg ctggatgctg tccgggaggt tgcccgggaa aatctgctgc aggagcgact
     4381 tcagcagatg gagcgggata tggtccgtga cctgcagaaa gagaaaacgc tgggcgggga
     4441 ctgatgctgt tgactcggcc gtttgtgtta accccataag gagattgctg atatggaaac
     4501 ttaccctgac cctgatgata tccggaaaaa cacagcagac atactgaaag cgttaaccgt
     4561 ggacaatatt cctgagcgtc atggcttcag ggaggagctg gcatcgctga aaaactgcat
     4621 aaacgatgat gagtattgct acatgacgtt ttatgaaacg gggtacgctt tcctgaaagc
     4681 cttactgaga acccgactga ggcttaaaag aaccgaccct gcgcattcgt tgctgcccct
     4741 catctcttcg tcggtggaag cactcaggac tcagcttaag gaaaatgaag cttatgtccg
     4801 ccttttaatc gggatggatg cggtctcccg atggaccggg cctctgttct gctttgccgc
     4861 gctgatgata ctgattctgg tgggaactgt gtttgcacac gtatggtttt aaagctcagt
     4921 aaggcgtgat gatatggaga atacgaataa tacagccagt gtggtcatga aaacccggga
     4981 gtggccggag cgtctgctgg tctggtcacc cggacagcgg gatatcatca aaacggtggc
     5041 actggtgctg atggtactgg accacatcaa tctgatattc cagctgaagc aggaatggat
     5101 gtttctggcc gggcgaggag cctttccgct gtttgccctg gtgtgggggc tgaatctgtc
     5161 ccgtcatgcg catatccggc aaccggccat taaccggctg tggggatggg ggattattgc
     5221 tcagttcgca tattatctgg caggctttcc ttggtatgag gggaatatcc tgtttgcctt
     5281 tgcagtggcg gcacaggtgc tgacgtggtg tgaaacgcgc agtgggtggc gtacagcagc
     5341 ggccattctt ctgatggccc tgtgggggcc tttgtccggc accagttacg gcattgccgg
     5401 gctgctgatg ctggcagtca gccaccggct gtaccgggcg gaagacagag cggaacgtct
     5461 ggcgctggtc gcctgcctgc tggctgttat tccggcgctt aaccttgcca ccagtgatgc
     5521 agcggcggtg gccggtcttg tgatgacggt gctgaccgtt ggtctggtgt cgtgtgcagg
     5581 aaaatcatta ccccgtttct ggcccgggga ttttttcccg gtattctacg cctgtcatct
     5641 ggctgtgctg ggcgttctgg cgctgtgacg ggtgtggtat ctttgaccgc aagaggatga
     5701 ttcgtcagag gcagaacaca gcatgacaga gcagaagcga ccggtactga cactgaagcg
     5761 gaaaacggaa ggggagacgc ctgtccgcag ccggaaaacc atcatcaatg tcaccacgcc
     5821 accaaagtgg aaggtgaaaa agcagaagct ggccgagaaa gcagcccggg aagcggagct
     5881 ggcggcaaaa aaagcgcagg ccagacaggc gctgtccatt tatctgaacc tgccctcgct
     5941 ggatgaggcc gtgaacaccc tgaaaccctg gtggccggga ttatttgacg gtgacacgcc
     6001 ccggcttctg gcctgcggta tccgggacgt gttactggaa gacgtggcgc agcggaatat
     6061 cccgctctcg cataaaaaac tgcgcagggc gctgaaggcc atcacccgtt cagaaagcta
     6121 tctgtgtgcc atgaaagccg gtgcctgccg gtatgatacg gaagggtatg tgacggagca
     6181 tatttctcag gaggaggaag cgtatgcggc agcgcgtctg gataaaatct gccgccagaa
     6241 ccggataaag gcagaacttc aggccgtgct tgatgagaaa taaaaaaaag cctccatcag
     6301 agaggaggca gggaaataag gcttaaaagg aaataacttc cgtacccgaa aggaagtcaa
     6361 ttcagtagat tatttaacac cataatactg acgggttaaa gaggagtgat aaagatgaat
     6421 ggattcagaa acagttcacg gaacggtcag gtctggcgct ggcagcgtgc cggaagccgg
     6481 gccgttattc tggaggtcag tggacgctgg atggaagcgg cagaagcatg gcgacgggct
     6541 gccggtgttg cgccccggac agactggcag cagtttgccc gaaaaagggc tgaacactgc
     6601 caccggcgca gccggggaag ggtgtaacga aaaaagcagc cttcattact ccagggattc
     6661 agaaggctga ataatgcatg agatttgctt tttttaatat ccggtcgggc agtatgaccg
     6721 gacagttctg agtagcacag agttttttcc gtattaagtc gtgattttat attttgtgat
     6781 taattttaca aaataaggtg ttattcagtg tgtgctgcaa tattcaggat tgcctgaaat
     6841 acaggtgcca tttatctgat atggagaata acatgaggaa atatattcca ctggtattat
     6901 ttatcttttc atggccggta ttgtgtgcag atattcatgg acgggttgtt cgtgtactgg
     6961 acggtgatac catcgaggtc atggactcac ggaaggcagt gcggatcagg ctgataaata
     7021 ttgatgcacc ggaaaagaaa caggattatg gccggtggtc aacggacatg atgaaatcac
     7081 tggttgcagg aaaaacggtt accgtcacat attttcagcg agatcgttat ggtcgtattc
     7141 tcgggcaggt ttatgcacca gacggaatga atgttaatca gtttatggtc cgggccgggg
     7201 ctgcctgggt atatgagcag tacaacacag atcccgtgct gcctgtcctt cagaacgaag
     7261 ccagacaaca aaaacgtggg ctctggtcag atgctgatcc ggttcccccc tggatatgga
     7321 gacaccgtaa ataaagcgat ggcaatctta tcattcatct gttctcagcg ggaataaaaa
     7381 tggcgaaaac aaaacaggaa tggctgtatc agttacggcg atgttcatca gtcaatacgc
     7441 tggaaataat tattcataaa aacagagaca gtttattaaa cagtgaacgt gaatcattta
     7501 attcagcagc agaccaccgg ttagcggaat taatcaccgg aaagttatat aacaggatac
     7561 caaaagagat atggaaatat gtaaggtaat aattcagcat aattaattac caggagcaga
     7621 taaacaatga agctgattat ttttattctc attgttctga ttattgcagc gttacttatc
     7681 cggattatcc tcagaagtgt aaatcagcat tctcctttgc tgatgcaatt gcatgcggca
     7741 ggtatacgga ccggtgatgc agagcgaata ctgtccggcg gtgaatactg gcaacgtcag
     7801 aagacgctgc tgacagaaag ggaagtcagt tttatgaaag gactgttcag aattgtggat
     7861 atgaagcggt ggtatctgtg tccgcaggta cgggtcgcag atatcgtcca gctgaacggg
     7921 aatatccggc cacgatcacg ccagtggtgg cagttattca ggatggtgtc tcagtggcat
     7981 gttgatgtgg ttatcgttga gcggcgttcg ttcagtattg ttgcagcagt agagctggat
     8041 gatgccagcc atttacgacc ggaacgcaga cgccgggata ttcttctgga agaggttctg
     8101 aggcaggctg gtattccgtt gctcagaagc cacgatgcca gaaaactgct gcagatgacc
     8161 ggagaatggc tgaaaacaac aggggctgat cagcagtccc cggaacatcg tagctgacgc
     8221 cttcgcgttg ctcagttgtc caaccccgga aacgggaaaa agcaagtttt ccccgctccc
     8281 ggcgtttcca taactgaaaa ccatactatt tcacagttta aatcacatta aacgacagta
     8341 attcccgttg atttgtgtgc caacacagat cttcgtcaca attctcaagt cgctgatttc
     8401 aaaaaactgt agtatcctct gcgaaacgat ccctgtttga gtattgagga ggcgagatgt
     8461 cgcagatcga aaatgcagta acttcctcac cgaaacgcat ttacagaaaa ggtaatccgt
     8521 taaccggagc cgagaaacaa cgcatatctg tttcccggaa aaaggggaca cataaagcga
     8581 ttaatgtttt catccagagt gaactcaaag atgatttgac acagctttgt aaggattcgg
     8641 gcttaactca aaaagaaatg atagagcact ggattttgaa agaaaaagct gcgatgactg
     8701 atgcgaatag aaagtaacta agttaccttc ttgaacattt gaattgcgga tgctagatta
     8761 ctgatcgttt aaggaatttt gtggctggcc acgccgtaag gtggcaggga actggttctg
     8821 atgtggattt acaggagcca gaaaagcaaa aaccccgata atctttctat gtttggcgac
     8881 gaagaagatt accggggccc act
//
LOCUS       DAZGXZ010000183         8881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000183 DAZGXZ010000000
VERSION     DAZGXZ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.183"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1555
                     /locus_tag="ACHKJF_004729"
     CDS             <1..1555
                     /locus_tag="ACHKJF_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0951963.1"
                     /translation="SNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFDKLDNDSQIDNDF
                     GLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNTTDTFIFTIIPDN
                     NHVLKLSSPITVTVECKGGYYFINSSGDKSDIIYKVDGLSIIARNFFTLLSGNFKPDW
                     RWDVSKETFTKEKFDSYVKPVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYAL
                     LVSGHIGIREKGWSLLCELLKHEEMASSAYKHKNNKVLYDLLNTRDMILNELHQHVFL
                     KDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHE
                     INFNGNYTARLANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGIIRDEEKKCYCLG
                     ALQVPFNQMKNPTDPEELANIFNKKDKEHMDDRFIHLIRSNAIASTPVYDNYFNNTTA
                     FRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGV
                     PGLSCFQPH"
     gene            1817..3439
                     /locus_tag="ACHKJF_004730"
     CDS             1817..3439
                     /locus_tag="ACHKJF_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0951964.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGDGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3629..3742
                     /gene="yhiY"
                     /locus_tag="ACHKJF_004731"
     CDS             3629..3742
                     /gene="yhiY"
                     /locus_tag="ACHKJF_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP0951965.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            3805..4872
                     /gene="yhiI"
                     /locus_tag="ACHKJF_004732"
     CDS             3805..4872
                     /gene="yhiI"
                     /locus_tag="ACHKJF_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0951966.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQISASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            4869..7604
                     /gene="rbbA"
                     /locus_tag="ACHKJF_004733"
     CDS             4869..7604
                     /gene="rbbA"
                     /locus_tag="ACHKJF_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP0951967.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMLWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDITHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            7604..8728
                     /gene="yhhJ"
                     /locus_tag="ACHKJF_004734"
     CDS             7604..8728
                     /gene="yhhJ"
                     /locus_tag="ACHKJF_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0951968.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
BASE COUNT         2335 a         1994 c         2263 g         2289 t
ORIGIN      
        1 ttcaaaccat caagataagc attcagataa agttaacagt catcaacacc atggcaaggt
       61 tgataaaaca catcgtgcaa agatagttga atttgataaa ctggataatg attcgcaaat
      121 agataacgat tttggcctgc atatcatcta tttcctgcaa catggtcact ggaaggtaaa
      181 tgatcgtagt caccagatgg agaaagtatg gttttataat agtgaacctt ctatagatat
      241 tcaagaatat aatagattcg ccgacaatac tactgatacg tttatcttta caattatacc
      301 agacaataac catgtgttaa aattatcgtc tcccattacc gttactgttg agtgtaaggg
      361 cggttattat tttattaatt cctctggaga taaatcagat ataatttata aagttgatgg
      421 gctttctatc atagcgagaa atttctttac tcttcttagt ggtaatttca aacctgactg
      481 gcgttgggat gtctctaaag agacgtttac taaggaaaag tttgatagct atgttaaacc
      541 tgtgttttca aaaatagact tctataaaca gtgtggtgtg attaacccac aaaatgccaa
      601 cacggcttat tttggcgata cagatggaag agtaggcgct gtactttatg cattgctcgt
      661 ttcagggcat ataggcatca gggaaaaggg ttggagttta ttatgtgaat tattaaaaca
      721 cgaagagatg gcttcatctg catacaaaca taaaaataat aaagtgctct acgatttatt
      781 aaatacgaga gacatgatct tgaatgagtt gcatcaacat gtttttttaa aggacgatgc
      841 gataacccct tgtatcttcc ttggggatca tacaggcgat agatttagta ctatttttgg
      901 tgataaatat atccttactt tgctaaattc tatgcgaaat atggagggta ataaagatag
      961 ccgtattaat aaaaatgtcg tcgttctggc tggaaatcat gaaatcaatt ttaatggtaa
     1021 ctatactgca cgactagcaa atcataaact ctctgccggt gatacgtata acttaattaa
     1081 aacgctcgat gtttgtaatt atgactcaga aaggcaagtt ttaaccagtc atcacggaat
     1141 aataagagat gaagaaaaaa aatgttattg tctcggtgct ttgcaggttc catttaatca
     1201 gatgaaaaat cctactgatc ctgaggaatt agccaatatt tttaataaaa aagataaaga
     1261 acacatggat gatcgtttta tccatctaat tagaagcaat gctatagcat ccacacctgt
     1321 atatgacaat tattttaata acactactgc tttcagacca aaacctgaag atatcttcaa
     1381 atgtggacag acgctcaaaa aaactaaaca gaaatatggc cattatggat taggggtaga
     1441 tcagcatcaa aaaattgata attataccat ggggctaaat tcctggaaga tagctcctaa
     1501 cgaacgtggt gataaaaaag gtgtcccagg gcttagttgt tttcaaccac attaaaactc
     1561 catgagtgct gtggtatatc tttttaatcc tgccagtatt tcataggttt tcttaatcga
     1621 ttgtagaggc atgttctgat gacgcctcta caatctttag ggactttctc tgctatgaga
     1681 taagaaattt catattatgc tttaaggctg aatgatttgt gcaaaatata attatttttc
     1741 catcgaacat gaggtgtatg tcataagatt aaatgttaac tatgcattcc tcgcggtgaa
     1801 ttatatgagg taaaagatga aaattggaac tgtggcaggc actaacgaca gtacaacgac
     1861 aatcgcaaca aatgatatgg tgcaagagca tgttactaac ttcactaaag aactgtttgg
     1921 gtatattgca aatggtatag gtgatgatat atcaagtatt gccaggacaa tgctcggtga
     1981 agttgtggaa aaaattgatg attggcaaat agaacgtttc caacaatcta ttcgagatga
     2041 caaaatatca tttaccatac aaacgaatca ttcagaaaaa tatagtatgc tatcggacat
     2101 gcgcgctcat attctacgta gggataataa ttatcagttt attgtgacaa ttaatagtaa
     2161 aaattacggc tgctatcttg ataataccga tattaattgg tgcagcattg tttatttact
     2221 taataatatg acagtaaatg atgatgctaa tgatgttgca gttactgagt cgtacaaacc
     2281 cgtctgggac tggaaaatat cgcaattcaa tgtatctgat attaaattcg aaacaatgat
     2341 aaacccccag ttcgctgaca gaacctattt ctccaattgt tcacctgttg atcccacaag
     2401 taccagacca acctattttg gcgataccga tggctcagtc ggtgcagttt tgtacgcgct
     2461 ttttgcaacg ggacatttga gaatcatggc tgaaggagaa aatttcctta gccagttgct
     2521 taatatagaa gatgaagttc tcaacgtatt gctaagggag aactttaatg agcaacttga
     2581 cactaatgtt aatacaataa tcagtattct gaacagaaga gatatagttt tagaaagtct
     2641 tcagccttat ttagttatta ataaagacgc tgtgacgcca tgtacgtttc ttggcgacca
     2701 aaccggcgat cgatttagta atatctgtgg cgatcaattt atcatcgatt tgttaaaacg
     2761 cataatgagt atcaatgaaa atgttcatgt attagctgga aatcatgaaa cgaattgcaa
     2821 tggtaactat atgcagaact tcacgcgtat gaaaccactt gatgaggata cctacgctgg
     2881 gataaaagat tatcctgtat gtttctatga ccccaaatac aaaataatgg caaatcatca
     2941 tgggataact tttgatgagc agcgaaaacg ttacattata ggaccgataa cagtctctag
     3001 tgatgaaatg accaatgctc tcgatccggt cgaattagcg gcaattatca ataaaaagca
     3061 ccatgctgta attaatggca aaaagtttaa aaatagccgg gctatctctt gtcgatcctt
     3121 taatcgttat ttttctgttt caacagatta cagacctaaa cttgaagcac tattagcatg
     3181 ttcacaaatg ttaggtataa atcaagttgt tgcacataac ggagatggtg gaagagaacg
     3241 cataggcgaa acggggacag ttttaggtct taacgcccgt gacagtaaac atgctggaag
     3301 aatgttcagc atgcataatt gccaaattaa ccctggtgca gggcctgaga taaccactcc
     3361 ctggaagtct taccagcatg aaaaaaacag aaatggactt atgcctttga ttagaaggcg
     3421 cacaatgtta cagctctgaa ccatagacaa catgaatatt aatgttacta tctggttgag
     3481 actgttgata aaacgtaaaa aggaatgctc tgttcctgca actgaacagg cgtgaaacgc
     3541 tatccaacag gatggatacc gttttgtcag ccagttttat agatcacact tatgagtatg
     3601 cgtgtttcat ttacggccag gaatgctgat gatgacaaca ttactacccg ttttcactaa
     3661 gccttccccg ttggcgctca atgctctgcg cgctggtcgt atttgtcgtt tccttcttat
     3721 cccggatgga agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc gtcgtttgct
     3781 tacagggatt atggcgggtc gattatggat aagagtaagc gccatctggc gtggtgggtt
     3841 gtcgggttac tggcggtggc ggctatcgtg gcgtggtggc tgttgcgccc ggcaggtgtg
     3901 ccggaaggct ttgctgtcag taatgggcgc attgaagcga cggaagtgga tattgccagc
     3961 aaaattgccg ggcgtatcga caccattctg gtgaaagaag gccagtttgt tcgcgaaggt
     4021 gaagtgctgg cgaagatgga tactcgcgtg ttgcaggaac agcgactgga agccatcgcg
     4081 caaatcaaag aggcacaaag cgccgttgct gccgcgcagg ctttgctgga gcaacgacaa
     4141 agcgaaactc gtgccgcaca gtcgctggtt aatcaacgcc aggcagaact ggactccgta
     4201 gcaaaacgtc atacgcgttc ccgttcactg gcccaacgag gggctatttc tgcgcaacag
     4261 ctggatgacg atcgcgccgc cgctgagagc gcccgagctg cgctggaatc ggcgaaagct
     4321 cagatatcgg cttctaaagc ggctatagaa gcggcacgca ccaatatcat tcaggcgcaa
     4381 acccgcgtcg aagcggcaca agccactgaa cggcgcattg ccgcagatat cgatgacagc
     4441 gaactgaaag ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc aggcgaagtg
     4501 ctggcggcag gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta tatgactttc
     4561 ttcctgccaa ccgaacaggc gggcacgctg aaactgggcg gtgaagcccg gctgatcctc
     4621 gatgccgcgc cagatctgcg tattccggcg accatcagtt ttgtcgccag tgtcgcccag
     4681 ttcacgccaa aaaccgtcga aaccagcgat gaacggctga aactgatgtt ccgcgtcaaa
     4741 gcgcgtatcc caccggaatt actccagcag catctggaat atgtcaaaac cggtttgccg
     4801 ggcgtagcgt gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct cgtggtgagg
     4861 ttgccgcaat gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa ctggcgggcg
     4921 tgagccagca ttatggaaaa aacgttgcgc tgaacaatat cactctcgat attccggccc
     4981 gctgtatggt cgggctgatt ggcccggacg gcgtcgggaa gtcgagcttg ttgtcgttga
     5041 tttccggtgc ccgcgtcatt gaacagggca atgtgatggt gctgggcggc gatatgcgcg
     5101 acccgaagca tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag gggctgggca
     5161 aaaatctcta ccacaccttg tcggtgtatg aaaacgtcga ttttttcgct cgcctgttcg
     5221 gtcacgacaa agcggagcgg gaagtgcgaa ttaatgagct gctgaccagc accgggttag
     5281 caccgtttcg cgatcgtccg gcagggaaac tctccggcgg gatgaagcaa aaactggggc
     5341 tgtgctgtgc gttaatccac gacccggaac tgttgattct tgatgagcca acaacggggg
     5401 ttgacccgct ctcccgcgcc cagttctggg atctgatcga cagtattcgc cagcggcaga
     5461 gcaatatgag cgtgctggtt gccaccgcct atatggaaga ggccgaacgc ttcgactggt
     5521 tggtggcgat gaatgccgga gaagtgctgg caaccggcag cgccgaagag ttgcggcagc
     5581 aaacgcaaag cgccacgctg gaagaagcat ttataaatct gttaccgcaa gcacaacgcc
     5641 aggcgcatca ggcggtagtg atcccaccgt atcaacctga aaacgcagag attgccatcg
     5701 aagcgcgcga tctgaccatg cgttttggtt ccttcgttgc cgttgatcac gtcaatttcc
     5761 gcattccacg cggggagatt tttggattcc ttggttcgaa cggctgcggt aaatccacca
     5821 ccatgaaaat gcttaccgga ctgctgcccg ccagcgaagg tgaggcgtgg ctgttcgggc
     5881 aaccggttga tccaaaagat atcgataccc gccgtcgggt aggctatatg tcgcaggcgt
     5941 tttcgctcta taacgaactc accgtgcggc aaaaccttga gttacatgcc cgtttgtttc
     6001 acatcccgga agcggaaatt cccgcgcgag tggctgaaat gagcgagcgt tttaagctca
     6061 acgacgttga agatgttctg ccggagtcat tgccgctcgg cattcgccag cggctttcgc
     6121 tggcggtggc ggtgattcat cgcccggaga tgttaatcct cgatgaacct acttctggcg
     6181 tcgatccggt ggcgagggat atgttatggc agttgatggt cgatctctcg cgccaggaca
     6241 aagtgactat cttcatctcc acccacttta tgaacgaagc ggaacgttgc gaccgcatct
     6301 cactgatgca cgccggaaaa gtgcttgcca gcggtacacc gcaggaactg gttgagaaac
     6361 gcggagccgc cagtctggaa gaggcattta tcgcctattt gcaggaagcg gcagggcaga
     6421 gcaacgaagc cgaagcgccg cccgtggtac acgacatcac ccacgcgccg cgtcagggat
     6481 ttagcctgcg ccgtctgttt agctacagcc gccgcgaagc actggaactg cgccgcgatc
     6541 cggtccgttc gacgctggcg ctgatgggaa cggtgatcct gatgctgata atgggttacg
     6601 gcatcagtat ggatgtggaa aacctgcgct ttgcggtgct cgaccgcgac cagaccgtca
     6661 gtagccaggc gtggacgctc aatctctccg gttcccgtta ctttatcgaa cagccgccgc
     6721 tcaccagtta tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg gtggcaattg
     6781 agatccctcc caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa ctcggcgtct
     6841 ggattgacgg agcgatgccc agtcgtgccg aaacggtaaa aggttacgtg caggcaatgc
     6901 accagagctg gttacaggat gtggcgagcc gacaatccac acccgccagc caaagcgggc
     6961 tgatgaacat tgagacgcgt tatcgctata acccggacgt aaaaagcctg ccagcgattg
     7021 ttccggcggt gatcccgctt ctgctgatga tgatcccgtc aatgctaagc gcccttagcg
     7081 tggtgcggga aaaagagctt gggtcgatta tcaaccttta cgtgaccccc accacgcgta
     7141 gtgaattttt gcttggtaaa cagttgccat acatcgcgct ggggatgctg aactttttcc
     7201 tgctctgcgg cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt ttcctgacgc
     7261 tcaccctggc ggcgctgctg tatatcatca ttgccaccgg aatggggctg ctgatctcca
     7321 cctttatgaa aagccagatt gccgccattt tcggaacggc gattatcacg ttgatcccgg
     7381 cgacacagtt ttccgggatg atcgatccgg tagcttcgct ggaagggcct ggacgttgga
     7441 tcggcgaggt ttacccgacc agtcattttc tgactatcgc ccgcgggacg ttctcgaaag
     7501 cgctggatct gactgatttg tggcaacttt ttatcccgtt gctgatagcc atcccgctgg
     7561 tgatgggctt aagtatcctg ctgctgaaaa aacaggaggg atgatgcgcc atttacgcaa
     7621 tatttttaat ctgggtatca aagagttgcg cagtctgctc ggtgataaag cgatgctgac
     7681 gctgattgtc ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta cgccaggatc
     7741 gttgaacctc gcgccgatcg ccattgccga tatggatcaa tcgcagttat cgaaccggat
     7801 cgttaacagc ttctatcgtc cgtggttttt gccaccggag atgatcaccg ccgatgagat
     7861 ggatgccgga ctggacgccg gacgctatac cttcgcgata aatattccgc ctaattttca
     7921 gcgtgatgtc ctcgccggac gccagccaga tattcaggtg aacgtcgatg ccacgcgcat
     7981 gagccaggca tttaccggca atgggtatat ccagaatatt atcaacggtg aagtgaacag
     8041 ctttgtcgcg cgctaccgtg ataacagcga accgttggta tcgctggaaa cccggatgcg
     8101 ctttaacccg aaccttgatc ccgcgtggtt tggcggggtg atggcaatca tcaacaacat
     8161 taccatgctg gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg aacacggcac
     8221 ggtggaacac ttgttggtga tgccgataac gccgtttgag atcatgatgg caaaggtctg
     8281 gtcgatgggg ctggtggtgc tggtggtgtc gggattatca ctggtgctga tggtgaaagg
     8341 cgtgctgggt gtaccgattg aaggctcgat cccgctgttt atgctgggcg tggcgctcag
     8401 tctgtttgcc accacgtcaa tcggcatttt tatggggacg atagcgcgtt caatgccgca
     8461 actggggctg ctggtgattc tggtgctgct accgttgcaa atgctttccg gtggatccac
     8521 gccgcgcgaa agtatgccgc agatggtgca ggacattatg ctgaccatgc cgacgacaca
     8581 ctttgttagc ctcgcgcagg ccatcctcta ccggggtgcc ggattcgaaa tcgtctggcc
     8641 gcagttcctg acgctgatgg caattggcgg cgcgtttttc accattgcac tgctgcgatt
     8701 caggaagacg attgggacaa tggcgtaaat acaattgccc gatgcgtaag cttataggga
     8761 ctacgcatcg gtgcaattta ttgaatttta gttgttttta ggccggataa ggcgtttatg
     8821 aactgacccc caaaacctgg acagtttcag ttaaccggct ttttgatact cagtcctgta
     8881 t
//
LOCUS       DAZGXZ010000184         8808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000184 DAZGXZ010000000
VERSION     DAZGXZ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.184"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..1353
                     /gene="moeA"
                     /locus_tag="ACHKJF_004735"
     CDS             118..1353
                     /gene="moeA"
                     /locus_tag="ACHKJF_004735"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP0951969.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            1353..2102
                     /gene="moeB"
                     /locus_tag="ACHKJF_004736"
     CDS             1353..2102
                     /gene="moeB"
                     /locus_tag="ACHKJF_004736"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP0951970.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(2178..2840)
                     /gene="fsa"
                     /locus_tag="ACHKJF_004737"
     CDS             complement(2178..2840)
                     /gene="fsa"
                     /locus_tag="ACHKJF_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0951971.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            2971..3870
                     /locus_tag="ACHKJF_004738"
     CDS             2971..3870
                     /locus_tag="ACHKJF_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP0951972.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            3876..6308
                     /locus_tag="ACHKJF_004739"
     CDS             3876..6308
                     /locus_tag="ACHKJF_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP0951973.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            6454..7269
                     /gene="ybiV"
                     /locus_tag="ACHKJF_004740"
     CDS             6454..7269
                     /gene="ybiV"
                     /locus_tag="ACHKJF_004740"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP0951974.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            7421..8686
                     /locus_tag="ACHKJF_004741"
     CDS             7421..8686
                     /locus_tag="ACHKJF_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP0951975.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         2090 a         2338 c         2360 g         2020 t
ORIGIN      
        1 aagatggata tggcatgtaa aggcaggagc caacgctacg cccgaggtgg catacagtct
       61 gtggcgtttt tctgacataa taggcaaatt cgattttgcc tccgcaggag tgttttcatg
      121 gaatttacca ccggattgat gtcgctcgac accgcgctta atgagatgct ttctcgcgtc
      181 accccactga ccgcccagga aacgctgcca ctggtacagt gttttggtcg tattctggcg
      241 agcgatgtcg tttcgccgct tgatgttccg gggtttgata actccgcaat ggacggctac
      301 gcggtgcgtt tagccgatat tgcctccagg caaccgctgc ccgttgccgg taaatccttt
      361 gccggtcagc cgtaccatgg tgaatggcct gcgggtacct gcattcgtat tatgaccggt
      421 gcgccggtgc cggaaggctg cgaagcggtg gtgatgcagg agcagactga acaaacggac
      481 aatggcgtgc gttttactgc tgaagtgcgt agcgggcaaa atattcgccg tcgcggtgaa
      541 gatatctctg caggtgcggt tgttttcccg gcgggaactc gcctgactac cgctgaactg
      601 ccagtgattg cttcactggg gattgccgaa gttccggtga ttcgtaaagt gcgtgtagcg
      661 cttttttcta ccggtgatga actccagttg cccggtcagc cactgggcga tggccaaatc
      721 tacgatacca accgtctcgc cgtacacctg atgttagaac agttgggatg cgaggtaatc
      781 aacttaggga ttatccgcga cgatccacat gccctgcgcg ccgcatttat tgaagccgac
      841 agccaggcgg atgtggtgat cagttccggc ggtgtttcag tgggtgaagc ggattacacc
      901 aaaacgattc ttgaagagct gggggagatc gccttctgga agctggcgat taaaccaggt
      961 aaaccgttcg cgttcggtaa actcagcaat agctggttct gcggcctgcc gggcaacccg
     1021 gtttcagcga cgctgacctt ctatcaactg gtacagcctt tgctggcaaa actaagcggc
     1081 aacaccgcca gcggcctgcc cgcgcgccag cgcgtacgca cagcgtcccg cctgaaaaaa
     1141 acgccaggac gtcttgattt ccagcgcggc gtgctgcaac gcaacgccga cggtgaactt
     1201 gaagtgacga ccaccggaca tcagggttca catatattta gctcctttag tctcggcaac
     1261 tgctttatcg tgctggaacg cgatcgcggc aatgtggacg tgggcgaatg ggtggaagta
     1321 gaaccgttta acgcgttgtt cggaggcctg taatggcgga actcagcgat caggagatgc
     1381 tgcgctacaa ccggcaaatc attctgcgcg gttttgattt tgacggtcag gaggcgctaa
     1441 aagattctcg tgtgctggta gtcggcctcg gtggactcgg ctgcgcagct tcgcagtatc
     1501 tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt cgacacggtt tcgctctcga
     1561 atctgcaacg ccagacgctg cacagtgatg ctacggtcgg gcaaccgaag gtagaatccg
     1621 cccgtgacgc catgacgcgg atcaacccac atatcgcgat tacgccagtc aatgcactgc
     1681 ttgatgacgc tgaacttgct gcgatgattg caaaacacga tctggtactc gactgtacgg
     1741 ataacgttgc ggtgcgtaat caactgaacg ctggctgttt tgccgcgaag gtgccgctgg
     1801 tttccggcgc ggcaattcgt atggaagggc aaatcacggt cttcacttat caggacggcg
     1861 aaccgtgcta tcgctgcctt agccgtttgt ttggcgagaa cgcattaacc tgcgtagaag
     1921 caggcgtgat ggcaccgttg atcggcgtaa ttggttcgtt gcaggcaatg gaagcgatca
     1981 aactgctggc aggttatggc aaaccggcca gcgggaaaat cgtcatgtac gatgcgatga
     2041 cctgtcagtt tcgcgaaatg aaactgatgc gcaatccggg gtgtgaggtg tgcgggcagt
     2101 aattgccacc tccaggccag ataagacgcg gcactccgcc acgtcatcag caatttatga
     2161 ggatgcagaa cgtccggtta aatcgacgtt ctgccaaacg ctcccagcca gtcctgttca
     2221 aacttcgcca cagcggcttc caccgccgga tagctaatca tctgttgtgc cacatccagt
     2281 ggcagcgtaa ttgattcaca tcctgccagt aagcagtcca gcgcctgacg cggggttttg
     2341 aaactcgctg ccagcacttt cgcctgcggc gcatgcattt tcaataactg gtgtaagtcg
     2401 gtcacagtct gaatgccgct accgccctga gcatcaatac gattaacgta aggcgcaaca
     2461 tattccgcac ctgccagcgc cgacagcagc ccttgtgctg cgccatatac cgcggttccc
     2521 agcgttggaa tcccttccgc ttttaacatc ttaatagctg ccagcccctc ggcggtcacc
     2581 ggaactttca ccacgatatc cgcaataata gaacgcaatt tacgtgcgtc attcaccatc
     2641 ccttcagcag tggtagccat tacctgggca aacagacgcc cctgaccgcc catcgcttca
     2701 tgaagttgcg gaagcacaac atccagcggt tttttacccg cggcgataat gcttgggtta
     2761 gtggtcacac ccgccagcgg aaaaatacgt gacagcgcct tcaccgcaac aacgtctgaa
     2821 gtatccagat acagttccat aaccatcctc aaaatgtact ttttgtctga atgtagacta
     2881 tcacgggaaa actctctgaa cagttgacgc acatcaatct aactttcatt cgaaagtatt
     2941 ttaatgtttg aacgaaacag agaggcaact atgattttca atattcagcg ctactcgacc
     3001 catgatggcc ccggtatccg cacggtcgta tttcttaaag gctgttcgct gggctgccgc
     3061 tggtgtcaga acccggaaag ccgcgcccgc acgcaggatc tgctgtatga cgcacgactg
     3121 tgtctggaag gctgcgagct gtgcgctaag gccgcgccgg aagtgattga gcgcgcgctg
     3181 aatggtttgc ttattcatcg ggaaaagtta accccggagc atctgacggc gttaaccgac
     3241 tgctgtccga cacaggcatt aaccgtgtgt ggtgaagtga aaagcgttga ggagatcatg
     3301 acgaccgttc tgcgcgataa accgttttac gatcgcagcg gcggcggttt aacgctttcg
     3361 ggtggtgagc cctttatgca gccggaaatg gcgatggcgc tactgcaagc cagccacgag
     3421 gcaggcattc atactgcggt agaaacctgt ctgcatgtgc cgtggaaata tatcgcccct
     3481 tctctgccct atatcgatct gtttcttgcc gatttaaaac acgttgccga cgcgccgttt
     3541 aaacagtgga ccgacggtaa cgccgccaga gtactggata acctgaaaaa actcgccgca
     3601 gcgggcaaaa aaatcattat ccgcgtgccg ctgattcagg gctttaatgc cgacgaaacc
     3661 tctgtaaaag ccattaccga ttttgccgcc gacgagctgc acgttggcga aattcatttt
     3721 ctgccctacc acacgctggg catcaacaaa tatcacttac ttaatctgcc ctatgacgcc
     3781 ccggaaaaac cgcttgatgc gccagaactg ctcgactttg cccagcagta tgcctgccag
     3841 aaagggttaa ccgcgacctt acgaggataa caaccatgac cacactgaaa ctggacacgc
     3901 tcagcgaccg cattaaagcg cacaaaaatg cgctggtgca tattgtgaaa ccgccagtct
     3961 gtaccgagcg cgcgcagcac tataccgaga tgtatcaaca acatctcgat aagccgatcc
     4021 cggtacgtcg cgcgctggca ctggcgcatc acctggcgaa tcgcaccatc tggatcaaac
     4081 acgatgagtt gatcattggc aaccaggcaa gcgaagttcg cgccgcgccg atcttcccgg
     4141 aatatactgt ctcgtggatc gaaaaagaga ttgatgatct ggcagatcgt cccggtgctg
     4201 gctttgcggt gagcgaagag aacaaacgcg ttctgcatga agtgtgcccg tggtggcgcg
     4261 gtcagaccgt acaggatcgc tgctacggca tgtttaccga tgagcaaaaa ggtctgctgg
     4321 cgaccggaat cattaaagcg gaaggcaata tgacctccgg cgatgcgcac ctggcggtga
     4381 atttcccgct gctgctggaa aaaggtcttg atggtctgcg tgagaaagta gcggaacgtc
     4441 gctcgcgcat caatctgacg gtgctggaag atttacatgg cgaacaattc ctgaaagcga
     4501 ttgatatcgt gctggtggca gtcagtgaac acattgaacg tttcgctgcc ctggcgcgtg
     4561 aaatggctgc taccgaaact cgcgaaagcc gtcgcgatga actgctgacg atggcagaaa
     4621 actgcgatct tatcgcccac cagccgccgc agactttctg gcaggcgctg caactgtgtt
     4681 acttcatcca attgattttg cagatcgaat ctaacggtca ctcggtatcg tttggtcgta
     4741 tggaccagta tctctacccg tactatcgcc gcgacgttga actcaaccag acgctggatc
     4801 gcgaacacgc catcgagatg ctacatagct gctggctgaa actgctggaa gtgaacaaga
     4861 tccgctccgg ctcacactca aaagcctctg cgggaagtcc gctgtatcag aacgttacca
     4921 ttggcggaca aaatctggtt gatggtcaac caatggatgc agtgaatccc ctctcttacg
     4981 cgatcctcga atcctgcggt cgcctgcgtt ccactcagcc taacctcagc gtgcgttacc
     5041 atgcaggaat gagtaatgat ttcctcgacg cctgcgtaca ggtgatccgt tgcggcttcg
     5101 ggatgccggc gttcaacaac gacgaaatcg tgatcccgga atttattaaa ctcggtattg
     5161 aaccgcagga cgcttacgac tacgcagcga ttggttgtat cgaaaccgcc gtcggtggca
     5221 aatggggcta tcgctgtacc ggcatgagct ttatcaactt cgcccgcgtg atgctggcgg
     5281 cgctggaagg cgggcgtgat gccaccagcg gcaaagtgtt cctgccacaa gaaaaagcgt
     5341 tgtcggcagg caactttaac aatttcgatg aagtaatgga cgcgtgggat acgcaaatcc
     5401 gttattacac gcgtaaatca atcgaaatcg aatatgtcgt cgacaccatg ctggaagaga
     5461 acgtgcacga tattctctgc tcggcgctgg tggatgactg tattgagcga gcgaaaagta
     5521 tcaagcaagg cggcgcgaaa tatgactggg tttctggcct gcaggttggc attgccaacc
     5581 tcggcaacag cctggcggca gtgaagaaac tggtatttga acagggcgcg attggtcagc
     5641 aacagctggc tgccgcactg gcggatgact tcgatggcct gactcatgag caactgcgtc
     5701 agcggctgat taacggtgcg ccgaagtacg gcaacgacga tgatactgtc gatacgctgc
     5761 tggctcgcgc ttatcagacc tatatcgacg aactgaaaca gtaccataat ccgcgctacg
     5821 gtcgtggtcc ggttggcggc aactattacg cgggtacgtc atctatctcc gctaacgtac
     5881 cgtttggcgc gcaaactatg gcaacgccgg acgggcgtaa agcccacacc ccgctggcag
     5941 aaggcgctag tccggcctct ggtactgacc atctcggccc tactgcggtc attggctcgg
     6001 tgggtaaact gcctacggca gcgattctcg gcggcgtgtt gctcaaccag aaactgaatc
     6061 cggcaacgct ggagaacgaa tctgacaagc agaagctgat gatcctgctg cgcaccttct
     6121 tcgaggtgca taaaggctgg catattcagt acaacatcgt ttcccgcgaa acgctgctgg
     6181 aggcgaaaaa acatccggat cagtaccgcg atctggtagt gcgtgtcgcg ggttattccg
     6241 ccttcttcac cgcgctctct ccagacgctc aggacgatat catcgctcgt actgaacata
     6301 tgctgtaatc tcttcggccc ggctgcggcg tcgccgggct aaatcattca catcatcaat
     6361 ttcatcctta ctttcattcg aaatataatt tgtgctctgc gtcacattgt tattacactg
     6421 ttacggtttc attttcgagc caggagcaca cctatgagcg taaaagttat cgtcacagac
     6481 atggacggta cttttcttaa cgacgccaaa acgtacaacc aaccacgttt tatggcgcaa
     6541 tatcaggaac tgaaaaagcg cggcattgag ttcgttgtcg ccagcggtaa tcagtattac
     6601 cagcttattt cgttctttcc tgagctaaag gatgagatct cttttgtcgc ggaaaacggc
     6661 gcactggttt acgaacatgg caagcaactg ttccacggcg aactgacccg acatgaatcg
     6721 cggattgtta ttggcgagtt gctaaaagat aagcaactca attttgtcgc ctgcggtctg
     6781 caaagtgcat atgtcagcga aaatgccccc gaagcatttg tcgcactgat ggcaaaacac
     6841 taccatcgcc tgaaagctgt aaaagattat caggagattg acgacgtact gttcaagttt
     6901 tcgctcaacc tgccagatga acaaatcccg ttagtgatcg acaaactgca catagcgctc
     6961 gatggcatta tgaaacccgt caccagtggt tttggcttta tcgacctgat tattcccggt
     7021 ctacataaag caaacggtat ttcgcggtta ctgaaacgct gggatctgtc accgcaaaat
     7081 gtggtagcga ttggcgacag cggtaacgat gcggagatgc tgaaaatggc gcgttattcc
     7141 tttgcgatgg gcaatgctgc ggaaaacatt aaacaaatcg cccgttacgc taccgatgat
     7201 aataatcatg aaggcgcgct gaatgtgatt caggcggtgc tggataacac atcccctttt
     7261 aacagctgac ctctctaccg gagccgtgcg ctccggtttt cccttctttt tacatcctgt
     7321 acatcacatc aaatcattaa acttgcatta acttatttat aacttatatt gtcacatgca
     7381 aatcagaatg gtttcgaatg aaagctaatg agaggtggtt atggcttcta cttttaccag
     7441 cgacacattg cctgccgatc acaaagcagc tatccgtcag atgaagcacg cgctgcgggc
     7501 gcagcttggc gacgtccagc agatctttaa tcagctaagc gatgacattg ccacgcgagt
     7561 ggctgaaatc aacgcactca aagcacaggg cgatgccgtc tggccggtgc tgtcttatgc
     7621 cgatatcaaa gcaggtcatg ttactgcaga gcagcgcgaa cagattaaac gtcgcggttg
     7681 tgcggtgata aaaggccatt tcccccgcga acaagcgcta ggctgggatc agtcgatgct
     7741 ggactatctg gaccgcaacc gctttgacga ggtctacaaa ggccccggcg ataatttctt
     7801 cgggacgctc agcgcttcac gtcccgagat ttaccccatc tactggtcgc aggcgcaaat
     7861 gcaggcccgc cagagtgaag aaatggcgaa tgcgcagtcg tttctcaatc gtctgtggac
     7921 atttgaaagt gatggaaagc aatggtttaa cccggatgtg agcgtcatct accctgaccg
     7981 tatccgccgc cgtccgcccg gaacgacctc caaaggtctt ggagcgcata ccgactccgg
     8041 ggcgctggaa cgctggctgc ttccagcgta tcagcgcgtt ttcgccaacg tctttaatgg
     8101 caatctggcg caatatgatc cctggcatgc ggcacatcgt acggaagttg aagagtacac
     8161 ggtggacaac accaccaaat gttccgtgtt tcggacattc cagggctgga cagcgctctc
     8221 tgatatgctg cctggtcagg ggctgctgca cgttgtgccc attcctgaag ccatggcgta
     8281 cgtactgtta cgtccgctgc ttgatgatgt gccggaggat gaactgtgcg gcgtagcgcc
     8341 cggaagagtg ttgccggtat cagagcaatg gcatccactg ttaattgagg cgttaaccag
     8401 cattccaaaa ctcgaggccg gagactccgt ctggtggcac tgcgacgtca tccattccgt
     8461 tgcccccgtt gaaaatcaac agggttgggg caacgtgatg tacattcctg cggcaccgat
     8521 gtgcgagaaa aatcttgcct acgcgcacaa ggtgaaggcc gcactggaaa aaggcgcatc
     8581 gccgggcgac ttcccgcgcg aggactatga aacaaactgg gaaggacgct ttacgcttgc
     8641 cgacctcaac attcacggta agcgagcgtt gggcatggat gtttgagagt taatggaagt
     8701 gaattgatgt gggattaaga aaatcgattc gacgttactc agattgctga caagtgcgct
     8761 ttcttaatgc ctgatgcgct acgcttatct ggcctacaaa gtcctgca
//
LOCUS       DAZGXZ010000185         8774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000185 DAZGXZ010000000
VERSION     DAZGXZ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.185"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(350..613)
                     /gene="rpsT"
                     /locus_tag="ACHKJF_004742"
     CDS             complement(350..613)
                     /gene="rpsT"
                     /locus_tag="ACHKJF_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP0951976.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            716..934
                     /locus_tag="ACHKJF_004743"
     CDS             716..934
                     /locus_tag="ACHKJF_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP0951977.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            942..1883
                     /gene="ribF"
                     /locus_tag="ACHKJF_004744"
     CDS             942..1883
                     /gene="ribF"
                     /locus_tag="ACHKJF_004744"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP0951978.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1926..4742
                     /gene="ileS"
                     /locus_tag="ACHKJF_004745"
     CDS             1926..4742
                     /gene="ileS"
                     /locus_tag="ACHKJF_004745"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP0951979.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4742..5236
                     /gene="lspA"
                     /locus_tag="ACHKJF_004746"
     CDS             4742..5236
                     /gene="lspA"
                     /locus_tag="ACHKJF_004746"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP0951980.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHKJF_004747"
     CDS             5324..5773
                     /gene="fkpB"
                     /locus_tag="ACHKJF_004747"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0951981.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5775..6725
                     /gene="ispH"
                     /locus_tag="ACHKJF_004748"
                     /gene_synonym="lytB"
     CDS             5775..6725
                     /gene="ispH"
                     /locus_tag="ACHKJF_004748"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP0951982.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6791..7705
                     /gene="rihC"
                     /locus_tag="ACHKJF_004749"
     CDS             6791..7705
                     /gene="rihC"
                     /locus_tag="ACHKJF_004749"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP0951983.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            7872..8693
                     /gene="dapB"
                     /locus_tag="ACHKJF_004750"
     CDS             7872..8693
                     /gene="dapB"
                     /locus_tag="ACHKJF_004750"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP0951984.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         2013 a         2170 c         2484 g         2107 t
ORIGIN      
        1 tttacaagat ctaataagga gatatttatg aatggttaaa gtgtatagga atttaattca
       61 aaaatagagt ggtcttaagt ttaatacctc aatgtgtatc acaatatcca cattctttgt
      121 aggggagtct ggagattgag tagatattct tgttcagaat gtatcagccg atggttctac
      181 gattcttaag ccacgaagag ttcagaaagt gtaacggcat gtctgcaaaa gccagcatct
      241 tttgactatc tggcaaccgg tagtgtgttc tctcacgcat cataaaagca gcaggcataa
      301 aaaaacccgc ttgcgcgggc tttttcacaa agcttcagca aattggcgat taagccagtt
      361 tgttgatctg tgcagtcagg ttagccttat gacgtgcagc tttgtttttg tggatcagac
      421 ctttagcagc ctgacggtcc acgatcggtt gcatttcgtt aaatgctttc tgtgcagcag
      481 ctttgtcgcc agcttcgata gctgcgtata ctttcttgat gaaagtacgc atcatagagc
      541 gacggcttgc gttgtgctta cgagcctttt cagactgaat ggcgcgcttc ttagctgatt
      601 tgatattagc caaggtccaa ctcccaaatg tgttctatat ggacaattca aaggccgagg
      661 aatatgccct tttagccttc ttttgtcaat ggatttgtgc aaataagcgc cgttaatgtg
      721 ccggcactcg ttacgtagtg atggcgcagg attctaccag cttgcggggt gtgaatacag
      781 cttttccgcg ataaaaattg cagcaggcgg tcactttctt cccgtgattt gcgccatggc
      841 aatgaaaagc cacttctttc tgatttcggt actcaatcgc cggttaacct tgaccgctgt
      901 acaaggtata ctcggacgat tttcactgtt ttgagccaga catgaagctg atacgcggca
      961 tacataatct cagccaggcc ccgcaagaag ggtgtgtgct gactattggt aatttcgacg
     1021 gcgtgcatcg cggtcatcgc gcgctgttac agggcttgca ggaagaaggg cgcaagcgca
     1081 acttaccggt gatggtgatg ctttttgaac ctcaaccact ggaactgttt gctaccgata
     1141 aagccccggc acggctgacc cggctgcggg aaaaactgcg ttaccttgca gagtgtggcg
     1201 ttgattacgt gctgtgcgtg cgtttcgaca ggcgtttcgc ggcgttaacc gcgcaaaatt
     1261 tcatcagcga tcttctggtg aagcatttgc gcgtaaaatt tcttgccgta ggtgatgatt
     1321 tccgctttgg cgctggtcgt gaaggcgatt tcttgttatt acagaaagct ggcatggaat
     1381 acggcttcga tatcaccagt acgcaaactt tttgcgaagg tggcgtgcgt atcagcagca
     1441 ccgctgtgcg tcaggcgctg gcggatgaca atctggctct ggcagaaagt ttactggggc
     1501 acccgtttgc catctccggg cgtgtagtcc acggtgatga attagggcgc actataggtt
     1561 tcccgacggc gaatgtaccg ctacgccgtc aggtttcccc ggtgaaaggg gtttatgcgg
     1621 tagaagtgct gggcctcggt gaaaagccgt tacccggcgt ggcaaacatt ggaacacgcc
     1681 caacggttgc cggtattcgc cagcagctgg aagtgcattt gttagatgtt gcaatggacc
     1741 tttacggtcg ccatatacaa gtagtgctgc gtaaaaaaat acgcaatgag cagcgatttg
     1801 cgtcgctgga cgaactgaaa gcgcagattg cgcgtgatga attaaccgcc cgcgaatttt
     1861 ttgggctaac aaaaccggct taagcctgtt atgtaatcaa accgaaacac ggaaccgaga
     1921 atctgatgag tgactataaa tcaaccctga atttgccgga aacagggttc ccgatgcgtg
     1981 gcgatctcgc caagcgcgaa ccgggaatgc tggcgcgttg gactgatgat gatctgtacg
     2041 gcatcatccg tgcggctaaa aaaggcaaaa aaaccttcat tctgcatgat ggccctcctt
     2101 atgcgaatgg cagcattcat attggtcact cggttaacaa gattctgaaa gacattatcg
     2161 tgaagtccaa agggctttcc ggttatgact cgccgtatgt gcctggctgg gactgccacg
     2221 gtctgccgat cgagctgaaa gtagagcaag aatacggtaa gccgggtgag aaattcaccg
     2281 ccgccgagtt ccgcgccaag tgccgcgaat acgcggcgac ccaggttgac ggtcaacgca
     2341 aagactttat ccgtctgggc gtgctgggcg actggtcgca cccgtacctg accatggact
     2401 tcaaaactga agccaacatc atccgcgcgc tgggcaaaat catcggcaac ggtcacctgc
     2461 acaaaggcgc gaagccagtt cactggtgcg ttgactgccg ttctgcgctg gcggaagcgg
     2521 aagttgagta ttacgacaaa acttctccgt ccatcgacgt tgcttttcag gcggtcgatc
     2581 aggatgcact gaaagcaaaa tttgccgtaa gcaacgttaa cggcccaatc tcgctggtga
     2641 tctggaccac tacgccgtgg actctgcctg cgaaccgcgc aatctctatt gcacctgatt
     2701 tcgactatgc gctggtgcag atcgacggtc aggccgtgat tctggcgaaa gatctggttg
     2761 aaagcgtaat gcagcgtatc ggcgtgaccg attacaccat tctcggcacg gtaaaaggtg
     2821 cggagcttga gttgctgcgc tttacccatc cgtttatggg cttcgacgtt ccggcaatcc
     2881 tcggcgatca cgttaccctg gatgccggta ccggtgccgt tcacaccgcg cctggccacg
     2941 gcccggacga ctatgtgatc ggtcagaaat acggcctgga aaccgctaac ccggttggcc
     3001 cggacggcac ttatctgccg ggcacttatc cgacgctgga tggcgtgaac gtcttcaaag
     3061 cgaacgacat cgtcgttgcg ctgctgcagg aaaaaggcgc gctgctgcac gttgagaaaa
     3121 tgcagcacag ctatccgtgc tgctggcgtc acaaaacgcc gatcatcttc cgcgcgacgc
     3181 cgcagtggtt cgtcagcatg gatcagaaag gtctgcgtgc gcagtcactg aaagagatca
     3241 aaggcgtgca gtggatcccg gactggggcc aggcgcgtat cgagtcgatg gttgctaacc
     3301 gtcctgactg gtgtatctcc cgtcagcgca cctggggcgt accgatgtca ctgttcgtgc
     3361 acaaagacac ggaagagctg catccgcgta ctctcgaact aatggaagaa gtggcaaaac
     3421 gcgttgaagt tgacggcatc caggcgtggt gggatcttga tgcgaaagag atcctcggcg
     3481 atgaagctga tcagtacgtg aaagtgccgg acacattgga tgtatggttt gactccggat
     3541 ctacccactc ttctgttgtt gacgtgcgtc cggaatttgc cggtcacgca gcggacatgt
     3601 atctggaagg ttctgaccag caccgtggtt ggttcatgtc ttccctaatg atctccaccg
     3661 cgatgaaagg caaagcgccg tatcgtcagg tactgaccca cggctttacc gtggatggtc
     3721 agggtcgcaa gatgtctaaa tccatcggca ataccgtttc gccgcaggat gtgatgaaca
     3781 aactgggcgc ggatattctg cgtctgtggg tggcatcaac cgactacacc ggtgaaatgg
     3841 ccgtttctga cgagatcctg aaacgtgctg ccgatagcta tcgtcgtatc cgtaacaccg
     3901 cgcgcttcct gctggcaaac ctgaacggtt ttgatccagc aaaagatatg gtgaaaccgg
     3961 aagagatggt ggtactggat cgctgggccg taggttgtgc gaaagcggca caggaagaca
     4021 tcctcaaggc gtacgaagca tacgatttcc acgaagtggt acagcgtctg atgcgcttct
     4081 gctccgttga gatgggttcc ttctacctcg acatcatcaa agaccgtcag tacaccgcca
     4141 aagcggacag tgtggcgcgt cgtagctgcc agactgcgct gtatcacatc gcagaagcgc
     4201 tggtgcgctg gatggcacca atcctctcct tcaccgctga tgaagtgtgg ggctacctgc
     4261 cgggcgaacg tgaaaaatac gtcttcaccg gtgagtggta cgaaggcctg tttggcctgg
     4321 cagacagtga agcgatgaac gatgcgttct gggacgagct gttgaaagtg cgtggcgaag
     4381 tgaacaaagt cattgagcaa gcgcgtgccg acaagaaagt gggtggctcg ctggaagcgg
     4441 cagtaacctt gtatgcagaa ccggaactgt cggcgaaact gaccgcgctg ggcgatgaat
     4501 tacgatttgt cctgttgacc tccggcgcta ccgttgcaga ctataacgac gcacctgctg
     4561 atgctcagca gagcgaagta ctcaaagggc tgaaagtcgc gttgagtaaa gccgaaggtg
     4621 agaagtgccc acgctgctgg cactacaccc aggatgtcgg caaggtggcg gaacacgcag
     4681 aaatctgcgg ccgctgtgtc agcaacgtcg ccggtgacgg tgaaaaacgt aagtttgcct
     4741 gatgagtcaa tcgatctgtt caacagggct acgctggctg tggctggtgg tagtcgtgct
     4801 gattatcgat ctgggcagca aatacctgat cctccagaac tttgctctgg gggatacggt
     4861 cccgctgttc ccgtcgctta atctgcatta tgcgcgtaac tatggcgcgg cgtttagttt
     4921 ccttgccgat agcggcggct ggcagcgttg gttctttgcc ggtattgcga ttggtattag
     4981 cgtgatcctg gcagtgatga tgtatcgctc gaaggccacg cagaagctaa acaatatcgc
     5041 ttatgcgctg attattggcg gcgcgctggg caacctgttc gaccgcctgt ggcacggctt
     5101 cgttgtcgat atgatcgact tctacgtcgg cgactggcac ttcgccacct tcaaccttgc
     5161 cgatactgcc atttgtgtcg gtgcggcact gattgtgctg gaaggttttt tgccttctaa
     5221 agcgaaaaaa caataataaa ccctgccgga taaggcctac agattgctgc gaaatcgtag
     5281 gccgcatccg gcaaaaatcc tcaaaatata agagcaaacc tgcatgtctg aatctgtaca
     5341 gagcaatagc gccgtcctgg tgcacttcac gctgaaactc gatgatggca ccaccgctga
     5401 gtctacccgc aacaacggta aaccggcgct gttccgcctg ggcgatgctt ctctttctga
     5461 agggctggag caacacctgt tggggctgaa agtgggcgat aaaaccacct tctcgctgga
     5521 gcccgatgcg gcgtttggcg tgccgtcacc ggacctgatt cagtacttct cccgccgtga
     5581 atttatggat gcaggcgagc cagaaattgg cgcaatcatg ctttttaccg caatggatgg
     5641 cagtgagatg cctggcgtga tccgcgaaat taacggcgac tccattaccg ttgatttcaa
     5701 ccatccgctg gccgggcaga ccgttcattt tgatattgaa gtgctggaaa tcgatccggc
     5761 actggaggcg taacatgcag atcctgttgg ccaacccgcg tggtttttgt gccggggtag
     5821 accgcgctat cagcattgtt gaaaacgcgc tggccattta cggcgcaccg atatatgtcc
     5881 gtcacgaagt ggtacataac cgctatgtgg tcgatagctt gcgtgagcgt ggggctatct
     5941 ttattgagca gattagcgaa gtaccggacg gcgcgatcct gattttctcc gcacacggtg
     6001 tttctcaggc ggtacgtaac gaagcaaaaa gtcgcgattt gacggtattt gacgcgacct
     6061 gtccgctggt gaccaaagtg catatggaag tcgcccgcgc cagtcgccgt ggcgaagaat
     6121 ctattctcat cggtcacgcc gggcacccgg aagtggaagg gacaatgggc cagtacagta
     6181 acccggaagg gggaatgtat ctggtcgaat cgccggacga tgtgtggaaa ctgacggtca
     6241 aaaacgaaga gaagctctcc tttatgaccc agaccacgct gtcggtggat gacacgtctg
     6301 atgtgatcga cgcgctgcgt aaacgcttcc cgaaaattgt cggtccgcgc aaagatgaca
     6361 tctgctacgc cacgactaac cgtcaggaag cggtacgcgc cctggcagaa caggcggaag
     6421 ttgtgttggt ggtcggttcg aaaaactcct ccaactccaa ccgtctggcg gagctggccc
     6481 agcgtatggg caaacgcgcg tttttgattg acgatgcgaa agatatccag gaagagtggg
     6541 tgaaagaggt taaatgcgtc ggcgtgactg cgggcgcatc ggctccggat attctggtgc
     6601 aaaatgtggt ggcacgtttg cagcagctgg gcggtggtga agccattccg ctggaaggcc
     6661 gtgaagaaaa cattgttttc gaagtgccga aagagctgcg tgtcgatatt cgtgaagtcg
     6721 attaagtcat tagcagccta agttatgcga aaatgccggt cttgttaccg gcatttttta
     6781 tggagaaaac atgcgtttac ctatcttcct cgatactgac cccggcattg acgatgccgt
     6841 cgccattgcc gccgcgattt ttgcacccga actcgacctg caactgatga ccaccgtcgc
     6901 gggtaatgtc tcggttgaga aaaccacccg taacgccctg caactgctgc atttctggaa
     6961 tgcggagatt ccgctcgccc agggagccgc tgtgccgctg gtacgcgcac cgcgtgatgc
     7021 ggcatctgtg cacggcgaat cgggaatggc tggctacgac tttgttgagc acaaccgaaa
     7081 gccgctcggg ataccagcgt ttctggcgat tcgggatgcc ctgatgcgtg caccagagcc
     7141 cgttaccctg gtggccatcg gcccgttaac caatattgcg ctgttacttt cacaatgccc
     7201 ggaatgtaaa ccgtatattc gtcgtctggt gatcatgggc ggttctgctg gtcgcggcaa
     7261 ctgcacacca aacgccgagt ttaatatcgc tgccgatcca gaagcggctg cctgtgtctt
     7321 ccgtagcgga atcgaaatcg tcatgtgcgg tctggatgtc accaatcagg caatattaac
     7381 ccctgactat ctcgctactt tgccggagtt gaatcgcacc ggaaaaatgc ttcatgcact
     7441 gtttagccac taccgtagcg gcagtatgca aagcggtctg cgaatgcacg atctctgcgc
     7501 catcgcctgg ctggtgcgcc cggacctgtt cactctcaaa ccctgttttg tggcagtgga
     7561 aactcagggc gaatttacct caggcacgac ggtggttgat atcgacggtt gcctgggcaa
     7621 gccagccaat gtacaggtgg cattggatct ggatgtgaaa ggcttccagc agtgggtggc
     7681 tgaggtgctg gctctggcgt cgtaacctgt cacatgttat tggcatgcag tcattcatcg
     7741 actcatgcct ttcactgata tccctccctg tttatcatta atttctaatt atcagcgttt
     7801 ttggctggcg gcgtagcgat gcgctggtta ctctgaaaac ggtctatgca aattaacaaa
     7861 agagaatagc tatgcatgat gcaaacatcc gcgttgccat cgcgggagcc ggggggcgta
     7921 tgggccgcca gttgattcag gcggcgctgg cattagaagg cgtgcagctg ggcgcggcgc
     7981 tggagcgcga aggatcttct ttactgggca gcgacgccgg tgagctggct ggtgcaggga
     8041 aaacaggcgt taccgtgcaa agcagcctcg atgcggtaaa agatgatttt gatgtgttta
     8101 tcgattttac ccgtccggaa ggtacgctga accatctcgc tttttgtcgc cagcatggca
     8161 aagggatggt gatcggcact acggggtttg acgaagccgg taaacaggcg attcgtgacg
     8221 ccgccgccga tattgcgatt gtctttgccg ccaattttag cgttggcgtt aacgtcatgc
     8281 ttaagctgct ggagaaagca gccaaagtga tgggtgacta caccgatatc gaaattattg
     8341 aagcacatca tagacataaa gttgatgcgc cgtcaggcac cgcactggca atgggagagg
     8401 cgatcgccca cgcccttgat aaagatctga aagattgcgc ggtctacagt cgtgaaggcc
     8461 acaccggtga acgtgtgcct ggcaccattg gttttgccac cgtgcgtgca ggtgacatcg
     8521 ttggtgaaca taccgcgatg tttgccgata ttggcgagcg tctggagatc acccataagg
     8581 cgtccagccg catgacattt gctaacggcg cggtaagatc ggctttgtgg ttgagtggta
     8641 aggaaagtgg tctttttgat atgcgagatg tgcttgatct caatagttta taaccacaaa
     8701 atatttgtta tggtgcaaaa ataacacatt taatttattg attataaagg gctttaattt
     8761 ttggcccttt tatt
//
LOCUS       DAZGXZ010000186         8726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000186 DAZGXZ010000000
VERSION     DAZGXZ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.186"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1002
                     /locus_tag="ACHKJF_004751"
     CDS             <1..1002
                     /locus_tag="ACHKJF_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0951985.1"
                     /translation="VSVAQKSFGKEMAMIMFFAMVINIMIARFTPWKFIFLTGHHTLF
                     MSMMVAVILATAGMTGITLIAVGSLVVGVAMVFFPAIAHPYMKKVTGSDDVAIGHFST
                     LSYVLAGFIGSKFGNKEHSTEDMNVPKSLLFLRDTPVAISFTMSIIFLVTCLFAGADA
                     VKELSGGKNWFMFSIMQSITFAAGVYIILQGVRMVIAEIVPAFKGISDKLVPNARPAL
                     DCPVVFPYAPNAVLVGFLSSFAAGLIGMFTLYLLNMIVIIPGVVPHFFVGAAAGVFGN
                     ATGGRRGAILGAFAQGLLITFLPVFLLPVLGNIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(1219..1503)
                     /locus_tag="ACHKJF_004752"
     CDS             complement(1219..1503)
                     /locus_tag="ACHKJF_004752"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0951986.1"
                     /translation="MEQKTLSAEPRRSFSNEFKLQMVKLALQPRVSVARIARKHDIND
                     NLLFKWLRLWLIKGRISRRLPVRNSSGIGVELLPVEMTLDERPYPAFYAR"
     gene            complement(1924..2937)
                     /locus_tag="ACHKJF_004753"
     CDS             complement(1924..2937)
                     /locus_tag="ACHKJF_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFP0951987.1"
                     /translation="MSTRIYLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHSLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWMELEGDMLRLTGRYEYVKGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTTQ
                     WLAFNQRIMQGEASLSTLNQPEFYDPEIVFFADKLDAYTDQPEFRMIAPDGTTFVTRF
                     SSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(2949..4265)
                     /gene="fucP"
                     /locus_tag="ACHKJF_004754"
     CDS             complement(2949..4265)
                     /gene="fucP"
                     /locus_tag="ACHKJF_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0951988.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAGQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNQHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRNALAEVTES"
     gene            complement(4293..5213)
                     /gene="rbsK"
                     /locus_tag="ACHKJF_004755"
     CDS             complement(4293..5213)
                     /gene="rbsK"
                     /locus_tag="ACHKJF_004755"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0951989.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            5518..6300
                     /locus_tag="ACHKJF_004756"
     CDS             5518..6300
                     /locus_tag="ACHKJF_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFP0951990.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISDRPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(6302..6528)
                     /locus_tag="ACHKJF_004757"
                     /pseudo
     CDS             complement(6302..6528)
                     /locus_tag="ACHKJF_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(7208..7345)
                     /locus_tag="ACHKJF_004758"
     CDS             complement(7208..7345)
                     /locus_tag="ACHKJF_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0951991.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTRVNIDD
                     Y"
     gene            complement(7695..8261)
                     /locus_tag="ACHKJF_004759"
     CDS             complement(7695..8261)
                     /locus_tag="ACHKJF_004759"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0951992.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GLNKAIPSLMADFTGELHRLISEYLQCKRRRDKSVEPTPLYYLWSRETVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            complement(8289..8486)
                     /locus_tag="ACHKJF_004760"
     CDS             complement(8289..8486)
                     /locus_tag="ACHKJF_004760"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0951993.1"
                     /translation="MHQDDFCIVNPGGCIIHFPANGRYILDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
BASE COUNT         2442 a         2026 c         1920 g         2338 t
ORIGIN      
        1 gtttccgtag cgcagaaaag cttcgggaaa gaaatggcga tgattatgtt ctttgcgatg
       61 gttatcaaca ttatgattgc ccgtttcaca ccgtggaaat ttatcttcct gaccggtcat
      121 cacacgttgt ttatgtcgat gatggtagcg gtaattctgg caacagcagg catgaccggc
      181 attacgctta tagccgttgg ctctttggtt gttggcgtgg caatggtctt tttcccggct
      241 attgcgcatc cgtacatgaa gaaagtgacc ggttctgatg acgttgccat cggccacttc
      301 tcaacattat cttatgtatt ggccggtttc atcggcagca agtttggtaa taaagagcac
      361 tcgactgaag acatgaatgt gccgaaaagt ctgctgttct tgcgcgacac gccggttgct
      421 atctctttta ccatgagcat aattttcctg gtgacgtgcc tgtttgcggg tgcagatgcg
      481 gtgaaagagc ttagcggtgg taaaaactgg ttcatgttct ctatcatgca atccattacc
      541 ttcgcagctg gcgtgtacat cattctgcag ggtgtgcgca tggtgattgc ggagattgtc
      601 ccggcattca aaggtatttc agacaagctg gttccgaatg ccagacctgc tctcgattgc
      661 ccggtcgtct tcccttacgc acctaacgct gtactggttg gtttcctgag tagctttgct
      721 gcaggtttaa tcggtatgtt cacgttgtat ctgctgaaca tgatcgtgat tattcctggt
      781 gtggtgccgc acttcttcgt aggtgcagct gcaggggtat tcggtaatgc aacgggtgga
      841 cgtcgtggtg cgattttggg tgccttcgct cagggcttgc ttattacctt cctgccagta
      901 ttcttattgc ctgtgctggg taacattggt tttgctaaca ccaccttcag tgatgctgac
      961 tttggcgcac tgggtatttt gttagggatt atcgttcgct aatattttga tgacattaat
     1021 atcctccagg atttcaggaa cgtggaggat attaattcct cttatttttt cccatttttc
     1081 tttcttccag tcaggactgc atccgattaa tcgtaagcgc tccgggaagt acgtagcgta
     1141 aggattattt tacagctgag aagtttcagt gcagcagttc atgcacacgg ttcgacggct
     1201 agtcattgat cttcataatc acctcgcgta aaaagcgggg taaggccgct catccagcgt
     1261 catctccaca ggtaataatt caacgccaat gccagaagag ttccttaccg gaagacgccg
     1321 tgatatacgc cctttgatca gccagagcct gagccatttg aacagaagat tatcattgat
     1381 atcatgcttc cgggcaatac gggcaacaga aacccttggt tgtaaagcca gtttaaccat
     1441 ttgaagttta aactcatttg aaaatgacct gcggggttca gctgataatg ttttttgttc
     1501 cataacaggt gtccactagt tgaaaaggtg ggtacctatg ttaccaatac tggcttaatg
     1561 gctacataca gcagtcagtt tacgcataca cttttatagt gcactccggc ctcatgaata
     1621 taacggtgga ttaccgccga acgaatcgga aaatagatac tggaaaaatc tgaaattgtg
     1681 accagttttt gttgaccact acaaataaat aaaagagttg cagtgcttgc tcggtaagaa
     1741 aacaatggaa gttgagatcc taaaagaagc tgtggagtac accactctac agtagttgcg
     1801 caaatttgga gtggacgata atgtgatcgt ctataagggc aacgctatca tagtcttgtc
     1861 ctggcgggta aaaaaacgcg cttaccttaa cgataagcgc gccgctgttc aggccttaag
     1921 tggttattca attcctgtgg tgactgtaaa agtgcgcgtt tgctgcggtg caacctgaat
     1981 cagcgtgcca ttacgttgcg cggcaagata tccctcaggc cgacaggttg caggtaatgc
     2041 aaaggcggct acctgttgct cgccgttata aaggatccag cgtgtcacat aatttagttc
     2101 ggcactggag aaacgagtaa caaacgtagt gccatcggga gcgatcatgc gaaactctgg
     2161 ctgatctgtg taagcgtcca gtttgtctgc aaagaagaca atttctggat cataaaactc
     2221 cggttgattc agcgtcgaca gagaggcttc tccctgcata atccgttgat taaacgccag
     2281 ccactgagtg gtgggattaa catgcgaagg tactgattca cgcaacctta atatttcgtc
     2341 cggtatattc tgactgaacg tagcatttgg tatatatgca taattcatgt ggcacatata
     2401 ttgtagtggc atatctacag aagccagatt ggttacggcc atcttaatat cgaacagtgt
     2461 agaggatttg tgaaggacca ctgttggctg agccagataa tgatgcccga acccctttac
     2521 atactcgtaa cgcccggtaa ggcgtaacat atctccctct aattccatcc atgcttcatc
     2581 catcgcggca caggccattt caccgtgtag cagatgagta tcttccgcag atgggcagcc
     2641 attagccagc aaacctgaat gaaaggcaaa acagccatag gtctctatca cctctgtcgc
     2701 cggtttaggc tggcgaaaca tattgcacat ggtgagactg tgtccatcaa attgcgcatc
     2761 ccaaatcatc tgccccatcc agggaagaat aattaaatgt ccacgactgt ttgcaatttt
     2821 aagcccctcg acaccactgt catagcgaaa agacgtgaca gtaaaatcac tattttccag
     2881 caagatacga ggtttttcgc caaaaagcgc ccgccacaaa taaatacgcg tactcataac
     2941 gattctcctc aggactctgt gacttcagcc agtgcattac gtactttgct ttcacgccag
     3001 aagtagacac cgacatagac aaagcagagc atagaaacca agaatgaaag ctgtagtgag
     3061 tggaacatat ctgcaatata tccctgaatt gccggaacca ccgctgcgcc aacaatagcc
     3121 ataacaatga ctgctcctgc catttctgta tgttggttat caactgtatc cagtgttcct
     3181 gcatagatcg tcgcccagca agggccaaac aaaacactta ccaggacggc gacatagacc
     3241 gcactgaaac ttggagccag tgcaacatat gccagaaaca gcgcccctat aacggaatag
     3301 agaatcagga ctttttccgg attaaaacgt gtcataagga tgttggctat aaacttgcca
     3361 ataaagaagc aggcaaagct gtagaccatg aagtttgaag catcacgttc gttgatatcg
     3421 cccaactcca gcgccagacg gatggtaaat gaccatactg cgacctgcat acccacataa
     3481 aggaattgag ccacaatacc acgacgaaag cgcggatttc tagccagata gcgcagcgta
     3541 tccattgctg acgggcgttt atggtgactt gtctgtgcca ctttacaggt cgggaagcgg
     3601 gttaaaagga acaacaccat gaccacaacc agaatcataa tcatatactt atacggttca
     3661 agggtgttct ctaacatcag caccttaaag ttgtgaattt gcccggcgtt cattccggac
     3721 atctgcttct caaggctttc cccctcggag aaaaccagat atttgcccaa taaaatacca
     3781 gacgcagcac caatcggata aaaggtctgg ctgatattga gccgcaatgt ggcgtaggct
     3841 tttggaccaa tcatcgaact gtatgtgttc gctgcagttt caaggaagct caggccaatc
     3901 gcaatcgcaa aaatagccgc aaggaacata gtgtaggttg ccatatgcga ggcagggaaa
     3961 aaaagtgtac aaccaccaat atacagcgtc aggccaatta aaattgccac cttataactg
     4021 gtctttttaa tcacaaggga tgctggtatt gcaattaaaa aataaccgcc ataaaatgcg
     4081 ctctgcacca atgctgaagc aaagttactt agcgaaaata cacttttgaa ttgagtgatt
     4141 aatatgtcat ttaatgcagc tgcgcatccc cataacggga ataaacacga taacaaaata
     4201 aactggaaca agggagtctt attcagatac ccatcaggca tctgaatgat gtttttatcg
     4261 ttcatagtgc tacctttaac tgtgcaggat gattattcgt ttaaggttaa aaattcatta
     4321 aattgttcaa tgctcggata agatgattgc gtacctttcc ctgtgacgct gaaagcggca
     4381 aagagagcgg cttttttcaa agcggcttca acatcaccgc tttgaacata ataatgggaa
     4441 aagcaaccaa taaatgcatc accagcgcca ctagtatcaa cagcatttac tttgaatgca
     4501 ggaacatgga cttcctgatc gcgggtcatc cataatgcgc ctttttcgct catggtaaca
     4561 ataatattgt tcagcccttt atcaactaac gaacgcgcag ccaaacgaat atgatcataa
     4621 gtatcaaccg acatacctgt taatatttcc agttctgttt cattcgggat aaagaaatca
     4681 catttgcagg cataagacat atctaactca cgcaatgccg gagccggatt taataacact
     4741 tcaataccat ttttcttacc aaactcaatc gcgtggtaaa ctgtttccag ttgaacttcc
     4801 agttgtaaaa cgatcaattt gcattttttc agatcttctg cagctcgatc gatatcttcc
     4861 ggggaaagaa atttattcgc ccccttaatt attaaaatac tattgctcga gttagcatta
     4921 acaaagatcg gcgcaacacc actgctggta caggggactt tctcaacata agtggtatta
     4981 attccccaag attcaagatt acgaatagta ttatccgcaa aaatatcatc acctacttta
     5041 gtcagcatca ggacttttga attcaattta gccgccgcca ccgcttgatt agcacctttc
     5101 ccaccacatc cgattttgaa ggcaggtgct tccagagttt ctccttcttt aggcatctga
     5161 ttagtgtaag taatgagatc caccatattg gaaccaataa ctgcaatgtc catttcacta
     5221 cctcttataa actttcgcat aacaatggta tttaaataac attatcatgt tacttttgca
     5281 tcatttgtga ctgagatcgc gattagcaca tcaacccgat gtttatttaa tagacttcca
     5341 gtcttatcac tcaggccaac actatctaat tataagcaac ctaacaggat taataccgaa
     5401 aattcagcag gctataccct tttcatttca aagggtcggt cgtatagtat ggtaactaaa
     5461 acaatgttta ctaatgccat gatgttattt ttataacatt tttcggagga agaattgatg
     5521 gaaacgaagc aaaaagagcg tatccgacgt ttgattgaaa tacttaagaa aaccgacaga
     5581 atccatttga aagacgcggc acgaatgctg gaagtttctg taatgactat tcgtcgcgat
     5641 ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat tgtaatggtg
     5701 cataaacccg caccatccat gccagtaatc caggacgttc cgagaaatca tcgtgatgac
     5761 ttacctattg caattctggc cgccggaatg gttaatgaaa atgatctgat cttctttgat
     5821 aatggccagg agataccgct cgttataagc atgatcccgg atgcaatcac cttcactggc
     5881 atctgttact cacatcgtgt ctttgttgcg ttgaatgaaa aacctaatgt gacagcaata
     5941 ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag taactcttcg
     6001 ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt acatgatcac
     6061 tttggcgtca gctggtttaa tcccgaagat cttgccacta agcgtaaagc gatggcccgt
     6121 ggactacgga aaattttgct cgcccgccac gccttgtttg atgaagtagc ctctgccagc
     6181 ctcgcaccgc tctctgcatt tgatgttctg attagtgatc gtccgttacc ggcagattat
     6241 gttacgcact gccggaatgc ttctgtaaag atcattacac ctgattcaga agacgaatga
     6301 cttactgaaa aaacaccgta ctcttgttaa acatcgtcgg attggactga ttacgttgca
     6361 ctttcatcac atattccagc ttatcaattt ggcttatcat ctactccaga cgctggtcat
     6421 cattgaccaa tagtctgata tggcttttgt cgcggtcctg aatcggcagg cagaaaatga
     6481 cttcaacgtt aaaagggcga cgggaaaaag cccacaaagg tgagtcatta cgcccggata
     6541 ataaacaggc tcctccctgt tatggaatcc gttgtattgt ccataatgtc tgggttttta
     6601 atgcacactg gataacaacc ggacgaatct tcaaatatca ctggcgctga cagccggttt
     6661 gaattcatct cacaaccctg catagagcga atctcctgcc tgcgcgtcac tccactccat
     6721 ggtatcaact tcacactctt tatctgcggc tagtttcagt caccagataa gcatcgacta
     6781 tgaaaggtga gccatgacaa cataacgttg gtaacgctct gatgccttaa cggaagatgc
     6841 ctgccaccac agggaatgta aacgactgaa gtgtggcctt taatgccgtg aacggctcat
     6901 ggtctcctgg cacggttgcc gccccaacct gtaacaacat tccacagtac aatgtctgtc
     6961 agagccgtag cctcccatgc ctgttgtagt aactctacca gttacgaaca tccttcctca
     7021 aaagtgttgt cgtatctcgc atggaagaaa agatcctgac taagcagcaa cacacaacgt
     7081 attgcggaac ctgcatgttt ttcctgtaac tagtgtatta cgacatatgg taatagctac
     7141 ctgtgtggtt tcgctggata acaaggggat ttattcgcaa gtaaaatgcc tgataaaata
     7201 cacgaatcta gtaatcatca atatttactc tggtcgaatg aagtgtgaag tggattgcca
     7261 gcagatgcgg ccagtgatcc accgcctgct gaacaaaacg ccggatttcc cccggctctg
     7321 aaagtaaggc ttcggttatt tgcactattt tatctctgtt gaatttggtt aagtcggtgc
     7381 agactcatca acacaagtac ggttcgatgc aaacagctgt gactggcaat atgaaaggaa
     7441 tgatgaatca gtcaggatga caaagtgccg gctgaccgga ggggacgcag gaagattcac
     7501 ggggggacca gcaccaggga acagcgccac atcccgggtg gtactgcttt gatgcgcccg
     7561 gtgacacaaa gcccggattg ttccagacat cctgaatcaa acgtcccaga ttaggggcgt
     7621 cgaaatatgc ctctctgacc attatattcc ggcgtacagg tagcaggtca gaagtgacaa
     7681 tgcgtcacct gacgttaaaa gtcactacac ccaagatgac gttcaacagc accatgcgat
     7741 tcaatgtaag cccgggctgt ctgttccagt acaccaggct cagcgttgta tgtgttagct
     7801 gcatcaaata ccaacgacag cacttcagga tacacaacca gatgtgtaat ggagttatct
     7861 tcacccaata cttttcccca cgcctgctca atcagatttc tgagaaccac cacctcacga
     7921 ctcttacacc agacatcgtt attaagtagc agcaccataa gataaggagt ggtatcgtta
     7981 gccacagtct ccctgctcca gagataatat aaaggggtgg gctcaacaga tttatctcta
     8041 cgtcgcttac actgcaaata ttcagaaatg agtctatgca gttcaccagt aaaatcagcc
     8101 atcagagagg gaatggcctt attaaggccc tgccctggta ttaatttaaa ttgtaataat
     8161 ttaatttcag gatgtgtggc tgcagcccga tacagagttg caaggacaca cttttgccag
     8221 agggcgttac aggaaagctt aacgtttgat tctgtataca taataaatca ccttacagtt
     8281 acaacaggtc aaaaaccgct gtagccagag ttacgctggc ctgatgcttt agtaccgggc
     8341 ttcgtcagat aatccagacg ctccaataag cgctgatact gctcagggaa atcaggatca
     8401 tgaatatcca ggatgtaacg tccattagca gggaaatgaa taatgcagcc ccctggatta
     8461 acaatgcaga aatcgtcctg atgcactgat caatacggag aggactctcg cgtgtggttt
     8521 attgacacca cagtgcagat tcggcgaatc cgcgatcacg gtgcgatttc gttccacagc
     8581 acacaatcat gaccccgggt tttattcagg taagcaggat tgcggatatc cggtgtcgcg
     8641 cctttgtgtc acgaacgggg taggtgcgaa acaccggata aaatgcaggc tggcaatacc
     8701 tctgaacgcc ctgcgcagag cggata
//
LOCUS       DAZGXZ010000187         8519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000187 DAZGXZ010000000
VERSION     DAZGXZ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.187"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(260..466)
                     /locus_tag="ACHKJF_004761"
     CDS             complement(260..466)
                     /locus_tag="ACHKJF_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP0951994.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            779..868
                     /gene="kdpF"
                     /locus_tag="ACHKJF_004762"
     CDS             779..868
                     /gene="kdpF"
                     /locus_tag="ACHKJF_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP0951995.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            868..2541
                     /gene="kdpA"
                     /locus_tag="ACHKJF_004763"
     CDS             868..2541
                     /gene="kdpA"
                     /locus_tag="ACHKJF_004763"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP0951996.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2564..4612
                     /gene="kdpB"
                     /locus_tag="ACHKJF_004764"
     CDS             2564..4612
                     /gene="kdpB"
                     /locus_tag="ACHKJF_004764"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP0951997.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVTRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRQMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4621..5193
                     /gene="kdpC"
                     /locus_tag="ACHKJF_004765"
     CDS             4621..5193
                     /gene="kdpC"
                     /locus_tag="ACHKJF_004765"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP0951998.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVKLNLALDKLDE"
     gene            5186..7870
                     /gene="kdpD"
                     /locus_tag="ACHKJF_004766"
     CDS             5186..7870
                     /gene="kdpD"
                     /locus_tag="ACHKJF_004766"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP0951999.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7867..>8519
                     /gene="kdpE"
                     /locus_tag="ACHKJF_004767"
     CDS             7867..>8519
                     /gene="kdpE"
                     /locus_tag="ACHKJF_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP0952000.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     "
BASE COUNT         1778 a         2213 c         2472 g         2056 t
ORIGIN      
        1 gccggttttc cgctcatctg ctcatccttg ttttatcggg ttatttgtct gcttccaggc
       61 aaaaccctac agatttataa gaaagcctta agtgagatat ctgtcagata agaacaatta
      121 ggaaaatatt tatatatatt tcatgaatat atgtttcgat tggtataacg atgtctaaat
      181 attcctctga acatcttcct gatacaaaaa accgccagcg tttccgctgg cggttttcaa
      241 tgtattcaaa actcagcaat caataaaaat caccagttgc cttttcggcc tgatccatcc
      301 acaccggttt atcgctggtt ttcgaccaga cgcgatgcag gtagctgtaa aagcgggcgc
      361 ggtctttcca gaaaagcatg aaaggcagcg ccagaacgcc cgctgcaacc gcataagtac
      421 ggcgtaaaaa gataagccat gcaggatatt ctctgtagag ttccatactt ttctccccct
      481 tttgtatcta cccggtgaat ggcaccggaa aaatgaattt gtttatctga tgaaaatagt
      541 accgcctttt gtgtaatttt actactcatc cgaccactta tttttgctta ttgatggttt
      601 atttacattc accctgtaat taagttacac aaaagttaag ttaatactaa acattagtta
      661 aatcatggct tttgccattt ttatactttt tttacacccc gcccgcagat ttttgcgaaa
      721 tctttgcagc cagaattcta cccttccggt atcactttta ggccactgga ggtgcactgt
      781 gagtgcaggc gtgataaccg gcgtattgct ggtgttttta ttactgggtt atctggttta
      841 tgccctgatc aatgcggagg cgttctgatg gctgcgcaag ggttcttact gatcgccacg
      901 tttttactgg tgttaatggt gctggcgcgt cctttaggca gcgggctggc gcggctgatt
      961 aatgacattc ctcttcccgg tacaacgggc gttgagcgcg tactttttcg cgcacttggc
     1021 gtctctgacc gtgagatgaa ctggaagcaa tatctttgtg ccattctcgg cctgaacatg
     1081 ctggggctgg cggtgctgtt ttttatgttg ctcggtcagc actatctgcc gcttaatcca
     1141 cagcagttgc cggggctgtc gtgggatctg gcgctgaata ccgccgtcag ctttgtcacg
     1201 aataccaact ggcaatctta cagcggtgaa accacgttga gctatttcag ccagatggct
     1261 ggcttaacgg tgcaaaactt tctttctgcc gccagcggga ttgcggtgat ttttgccctc
     1321 atccgtgcgt ttacccgcca gagcatgagc acactcggga atgcctgggt cgatctgcta
     1381 cgcatcacgt tatgggtgct cgtccctgtg gcgttgttga tcgcactgtt ttttattcaa
     1441 caaggtgcgc tgcaaaactt tcagccttat caggctgtga ataccgttga aggagcgcaa
     1501 cagctgttac ccatggggcc tgtagcttct caggaagcga tcaagatgct cggtactaac
     1561 ggcggtggct tctttaatgc caactcgtcg catccgtttg aaaacccaac cgcactgacc
     1621 aacttcgtgc agatgctggc gatcttcttg atcccaacgg cgctgtgctt tgcctttggt
     1681 gaagtgacgg gcgatcgccg ccaggggcgc atgttgctgt gggcgatgtc agtgattttt
     1741 gtcatctgcg taggcgtggt gatgtgggca gaagttcagg gtaatcctca tctgctggcg
     1801 ctcggcgcag acagcagcat caatatggaa ggtaaagaga gccgtttcgg cgtgctggtc
     1861 agtagcctgt ttgcggtcgt gactacggcg gcttcctgtg gcgcggtgat tgcgatgcat
     1921 gattcgttta ccgctctcgg tggcatggtg ccgatgtggc tgatgcaaat tggtgaagtg
     1981 gtgttcggcg gtgtcggttc tggtctttac ggcatgatgc tgtttgtcct gctggcggtg
     2041 tttattgccg ggctgatgat tggtcgtaca ccggaatatc tgggtaaaaa aatcgacgta
     2101 cgcgagatga aactgactgc actggcgatt ctggttaccc cgacgctggt gctgatgggc
     2161 gcggcgctgg caatgatgac cgacgccgga cgtagcgcca tgctcaaccc tggcccgcat
     2221 ggttttagcg aagtgctgta cgccgtgtca tccgccgcta acaacaacgg cagcgccttt
     2281 gccggattaa gcgccaactc cccgttctgg aactgtttac tggcgttctg catgtttgtc
     2341 ggtcgcttcg gggtgattat cccggtgatg gcaattgccg gttcgctggt gagtaaaaag
     2401 agccaacccg ccagctccgg cacgctgcca acgcacggcc cgctgtttgt tggcctgtta
     2461 atcggcaccg tgttgctggt tggcgcactg acctttatcc ctgccctggc gcttggtccg
     2521 gtggcggaat atctctcctg atgatattga gtgagcactg aatatgagtc gtaaacaact
     2581 ggcgctattc gaaccaacac ttgtcgttca ggcgctgaaa gaagcggtga aaaaattaaa
     2641 cccgcaggcg caatggcgca atccggtgat gtttatcgtc tggatcggca gtctgctgac
     2701 cacctgtatt agcatcgcga tggcaagcgg tgcgatgcct ggcaatgcgc tgtttagcgc
     2761 ggcaattagc ggctggctgt gggtcaccgt actgttcgct aatttcgccg aggcgctggc
     2821 agaaggccgc agtaaagcgc aggccaacag tcttaaaggg gtgaaaaaaa ctgcctttgc
     2881 ccgcaagctg cgtgagccga aatatggcgc tgcggcggac aaagttcctg ccgatcaact
     2941 gcgtaaaggt gacatcgtgc tggtagaagc tggcgatatt atcccctgcg atggtgaagt
     3001 cattgaaggc ggcgcatcgg tcgatgaaag cgccatcacc ggggaatcgg caccggtgac
     3061 ccgtgaatcc ggcggcgatt ttgcctccgt caccggcggc acgcgtattc tttctgactg
     3121 gctggtgatt gagtgtagcg ttaaccccgg tgagacattt ctggatcgga tgatcgcgat
     3181 ggtggaaggc gcacagcgac gcaaaacacc gaacgagatt gccctgacta tcctgctgat
     3241 tgctctgacc atcgtctttt tactggcaac cgccacgctg tggccgtttt ccgcgtgggg
     3301 cggtaatgcc gtgagtgtaa cggtactggt ggcgctgctg gtctgtctga tcccaaccac
     3361 tattggcggc ctgttgtcag cgatcggcgt cgccgggatg agccggatgc taggcgcgaa
     3421 tgtgattgcc accagcggac gtgcagttga agcggcaggt gacgttgacg ttctgctact
     3481 ggataaaacc ggcaccatca cactcggtaa ccgtcaggcg tcggagttta tccccgcgca
     3541 gggcgtggat gaaaaaacgc tggctgacgc cgcacaactg gcttcgctgg ctgatgaaac
     3601 gccggaaggc cgcagtattg tgatcctcgc caagcagcgt tttaacctgc gcgagcgcga
     3661 tgtgcagtcg ctccatgcca cctttgtacc gtttactgcg caaagccgga tgagcgggat
     3721 caacatcgac aaccgcatga tccgtaaagg ttctgtcgat gccattcgtc gccatgttga
     3781 ggctaacggt ggtcacttcc ctaccgatgt tgatcaaaaa gtcgatcagg ttgcgcgtca
     3841 gggagccacg ccgctggtgg tggtggaagg ttctcgtgtg ctgggcgtta ttgcgctgaa
     3901 agatatcgtc aaaggcggta ttaaagagcg cttcgcgcaa ctgcgccaaa tgggcattaa
     3961 aacggtgatg attaccggcg ataaccgtct gactgccgcc gccattgctg cggaagcggg
     4021 cgtcgatgat tttctcgccg aagcgacacc ggaggccaag ctggcattga ttcgtcagta
     4081 tcaggcggaa ggccgtttgg tggcgatgac cggcgacggc accaacgatg ctccggcgct
     4141 ggcgcaggca gatgtcgcgg tggcgatgaa ctccggtact caggcggcga aagaagcggg
     4201 caatatggtc gatctcgact ctaacccgac caagttgatc gaggtggtac atattggcaa
     4261 acagatgctg atgacccgtg gctcgctgac caccttcagc attgccaacg atgtggcgaa
     4321 atacttcgcc attattccgg cggcgttcgc ggcaacgtat ccgcagttaa atgcgctgaa
     4381 catcatgcgc cttcattcgc ccgactccgc cattctcagt gcggtcattt tcaacgcctt
     4441 gattatcgtc tttttgattc ctctggcgtt aaaaggcgtg agttataaac cgcttaccgc
     4501 ttctgccatg ttgcgccgta acttatggat ttacggtctg ggtgggctgc tggtgccgtt
     4561 tattggtatc aaagtcattg atttactgct gaccgtttgc ggtctggtgt gaggtttacc
     4621 atgcgtggat tacgtccggc attatcgaca tttatctttc tgttattgat tactggcggc
     4681 gtttacccgc tgctgaccac cgtgctgggg caatggtggt ttccctggca ggccaacggt
     4741 tcgttgatcc gtgaaggtga tacagtgcgg ggttcggcat taatcgggca gaattttacc
     4801 ggcaacggct attttcatgg tcgcccgtcg gcaacggcag aaatgcccta taatccacag
     4861 gcttctggcg ggagcaatct ggcggtcagt aaccctgagc tggataaact aatagccgca
     4921 cgcgttgctg cattacgggc cgctaacccg gatgccagcg cgagcattcc ggttgaactg
     4981 gtgacggcat cggcaagcgg gctggacaat aatatcaccc cgcaagcggc ggcctggcag
     5041 atcccacgca tcgcgaaagc gcgtaatctc agcgttgaac agctcacgca actgatcgca
     5101 aaatacagcc aacaaccact ggtgaaatat atcggccagc cggtcgtcaa cattgtcaaa
     5161 ctcaatctgg cgctggataa acttgatgaa taacgaaccc ttacgtcccg accccgatcg
     5221 tctgctggaa caaactgccg cgccgcatcg ggggaagctg aaagttttct tcggtgcctg
     5281 cgcaggcgtc gggaagacct gggcgatgct ggcagaagcc cagcgactgc gggcgcaagg
     5341 gctggatatt gtggttggcg tggtagaaac ccacgggcgg aaagataccg ccgccatgct
     5401 ggaagggctg gctgttctgc cgccaaaacg cctggcgtac cgtgggcggc atatcagcga
     5461 gtttgatctc gatgccgccc tcgcccgccg tccggcgctg atcttaatgg acgaactggc
     5521 gcacagtaat gcgcctggtt ctcgtcatcc caaacgctgg caggatattg aagagctgct
     5581 ggaagcaggc attgatgttt ttactaccgt caacgttcaa catctggaaa gcctgaatga
     5641 tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc gtgcccgatc cttttttcga
     5701 tgccgccgac gacgtagtgc tggtggactt gcccccggac gatctgcgcc agcggctgaa
     5761 agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc attgaacatt ttttccgcaa
     5821 aggcaatctg atcgccctgc gtgaactggc actgcgccgt actgccgatc gcgtagatga
     5881 gcaaatgcgc gcctggcggg ggcatcctgg tgaagagaaa gtgtggcaca cgcgcgacgc
     5941 gatcctttta tgcatcgggc ataacaccgg cagtgagaaa ctggtccgcg cggcggcgcg
     6001 gttggcctcg cgcctgggta gcgtctggca cgcggtgtat gttgaaaccc ctgccctgca
     6061 tcgcttaccg gaaaaaaaac gtcgggcaat tctcagcgcc ttacgtctgg cgcaggagct
     6121 gggcgcggag acggctacac tttctgatcc agcggaagaa aaagcagtag tgcgttatgc
     6181 ccgtgaacat aatctcggca agattattct cggtcgcccg gcctcgcgcc gctggtggcg
     6241 tcgggaaacg tttgctgacc gactggcgcg catcgccccc gatctcgatc aggtgctggt
     6301 cgcgcttgat gaaccacccg cccgcacgat taacaacgcg ccggatagcc gctcttttaa
     6361 agacaagtgg cgggtacaga ttcagggatg cgtggttgcc gccgcgttat gcgccgttat
     6421 cactttaatt gccatgcagt ggctgatggc gtttgatgcc gccaacctgg tgatgctgta
     6481 tctgcttggc gtggtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgctac
     6541 cgtcattaat gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgctgt
     6601 ctctgatgtg caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg
     6661 gaaccttact gcgggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac
     6721 acggcactta tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc
     6781 cgccaccagc gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc
     6841 cgatgacaac ggtaaattgc agccgttaac acatccgcaa ggaatgacgc catgggacga
     6901 tgccatcgcg cagtggagtt ttgataaagg cctgcctgcg ggtgcgggca ccgacacgtt
     6961 acccggcgtg ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acggactggt
     7021 ggtggtggaa ccggggaatc tgcgtcagtt gatgatcccg gagcagcagc gcctgctaga
     7081 gacgtttacg ctgttagtcg ccaatgcgtt tgagcggctg acgctgaccg ccagtgaaga
     7141 acaggcgcgg atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cggcgctttc
     7201 gcatgattta cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga
     7261 tctggcaagc gaaggatcgc cccacgcccg ccaggccagc gagattcgtc agcatgtgct
     7321 gaacactacc cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt
     7381 taatttgaag aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgct
     7441 ggaaccgggt ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca
     7501 cgttgacggg ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata
     7561 tgcgggtgcg caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact
     7621 ggatgtctgg gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa
     7681 gtttgctcgc gggaataaag agtcggcagt accgggggta gggcttggac tggcaatttg
     7741 tcgggcgata gtggatgtac acgggggcac tattaccgcg ttcaaccgac cggaaggtgg
     7801 tgcctgtttt cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga
     7861 ggatatgtga caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc
     7921 acggcgctgg agggcgacgg gatgcgcgtc tatgaggccg aaacgctgca acgcggcttg
     7981 ctggaagcgg ctacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt
     8041 gatgggattg agtttatccg cgacctgcgc cagtggagcg cggtgccggt gattgtgctt
     8101 tccgcacgca gcgaagagag cgacaaaatc gccgcgctgg atgccggagc ggatgattat
     8161 ctgagtaagc cgtttggcat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc
     8221 cactccgcca ccgccgcgcc cgatccgctg gtgaaatttt ccgatgttac cgtcgattta
     8281 gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc tcacgccgat tgagttccgc
     8341 ctgctggcgg tgctgctgaa taatgccggg aaagtgctca cccagcgcca gttactcaat
     8401 caggtgtggg ggccaaacgc ggtcgaacac agtcactatt tgcgtattta tatgggacat
     8461 ctgcgacaaa aactggaaca ggatcccgct cgcccccgcc atttcattac tgaaaccgg
//
LOCUS       DAZGXZ010000188         8485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000188 DAZGXZ010000000
VERSION     DAZGXZ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.188"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(10..669)
                     /locus_tag="ACHKJF_004768"
     CDS             complement(10..669)
                     /locus_tag="ACHKJF_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP0952001.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(833..1144)
                     /gene="yqfB"
                     /locus_tag="ACHKJF_004769"
     CDS             complement(833..1144)
                     /gene="yqfB"
                     /locus_tag="ACHKJF_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP0952002.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            1189..2622
                     /gene="bglA"
                     /locus_tag="ACHKJF_004770"
     CDS             1189..2622
                     /gene="bglA"
                     /locus_tag="ACHKJF_004770"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP0952003.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(2679..3422)
                     /locus_tag="ACHKJF_004771"
     CDS             complement(2679..3422)
                     /locus_tag="ACHKJF_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0952004.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(3683..6556)
                     /gene="gcvP"
                     /locus_tag="ACHKJF_004772"
     CDS             complement(3683..6556)
                     /gene="gcvP"
                     /locus_tag="ACHKJF_004772"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP0952005.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(6675..7064)
                     /gene="gcvH"
                     /locus_tag="ACHKJF_004773"
     CDS             complement(6675..7064)
                     /gene="gcvH"
                     /locus_tag="ACHKJF_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP0952006.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(7088..8182)
                     /gene="gcvT"
                     /locus_tag="ACHKJF_004774"
     CDS             complement(7088..8182)
                     /gene="gcvT"
                     /locus_tag="ACHKJF_004774"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP0952007.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT         2032 a         2307 c         2158 g         1988 t
ORIGIN      
        1 gcatacccat tacgcctgcc caatatacaa atagatcgcc agaaagtggc acacactgcc
       61 gccgagcacg aagccgtgcc agatggcatg attgtaggga atgcgtttgc agacatagaa
      121 aatcactccg agcgaataaa ccacgccgcc taccgccaat aaggtaacgc tgcccgccgc
      181 gagcttaact gccatttcat aaattaccac cagcgacagc cagcccatcg ccagataggt
      241 caccagagat aaaattttga atcggtgcgc gatggtcagt ttaaacagaa tccccagcaa
      301 tgccaggctc cagataacaa tcatcaaccc gcgcgctaac ggagaatcca gccccaccag
      361 caaaaacggc gtgtaggttc cggcaatcaa caggtaaata gcgcaatggt caaatttctt
      421 cagccacatt tttgcccgtt gatgaggaat ggcgtgatag agcgtcgaag cgaggaacag
      481 caggatcata ctgccgccat agaggctgta gctggttatc gctgtggcgc tggcattgag
      541 atccaccgcc tgaaccagta gcaacaccag cccaacgata ccaaacacca acccaatgcc
      601 gtgactgacg ctgttggcaa tttcctctgc cagcgaatat ccctgcttaa tgaggggctt
      661 ctgaaccata acttactccg gagaaacgta cacgcacatg tatacctctc cagcgtaact
      721 gagaatagtt ccagtgaaca cctgttagct aaaataaatt ctgatttgag actaatctcc
      781 taaaaatcat gaaattaaat gagaaatttc aactaacagg cgtgagttca atttaaagac
      841 atttaaattc aatcacataa aactgtgtct gaccgggata gatgtcggca atgacctttt
      901 tcagttcagt cagggtcata ttttcctgct ctgcatgttt ttcagtcagc gtatccagcc
      961 ttacggttga ggttgcggtg acttcaatcg tgcaaaaata accgtcatct tcaaaacgcc
     1021 cgacacgaag cacatcaccc gttttgaagt gcgattcaga ctcgtcgcgg atggtgatgg
     1081 ttttacgccc agccagaatg tcatcctgga aacgttgaaa aaaagtgatg tcgtttggct
     1141 gcatggtact atttcctgta agaattgact catctggagc ctatgatagt gaaaaaactc
     1201 accttaccga aagatttctt atggggcggc gcagttgccg ctcatcaggt cgaaggcagc
     1261 tggaacaaag gcggcaaagg gccgagcatt tgtgacgttc tgaccggtgg cgcacacggc
     1321 gtgccgcgcg aaatcaccaa agaagtcttg ccaggcaaat actatccaaa ccatgaagcc
     1381 gttgattttt atggtcacta caaggaagac atcaagctat ttgccgaaat gggcttcaaa
     1441 tgttttcgga cttccatcgc ctggacgcgt atttttccaa aaggtgatga agctcagcca
     1501 aacgaagaag ggctgaagtt ctacgatgat atgttcgatg aactgctgaa atacaacatc
     1561 gaaccggtga tcaccctctc ccactttgaa atgccgctgc atctggtgca gcaatacggt
     1621 agctggacca accgtaaagt ggttgatttc tttgtccgtt tcgcggaagt ggtgtttgaa
     1681 cgctataagc ataaagtcaa atactggatg accttcaacg aaattaacaa ccagcgtaac
     1741 tggcgtgcac cgctgttcgg ttactgctgc tccggcgtgg tttataccga gcatgacaac
     1801 ccggaagaga cgatgtacca ggtgctgcat caccagtttg tcgccagcgc cctggcggtg
     1861 aaagctgcgc gtcgcattaa cccggagatg aaagtcggct gtatgctggc gatggtgccg
     1921 ctctatccct actgctgtaa cccggacgat gtgatgttcg ctcaggagtc gatgcgcgaa
     1981 cgctacgtct ttaccgatgt gcagctacgc ggctattacc cgtcctatgt gttgaacgag
     2041 tgggagcgtc gcggatttaa catcaaaatg gaagacggcg atctcgatgt actgcgtgaa
     2101 ggcacctgcg attatcttgg tttcagctat tacatgacca acgcagtgaa ggccgaaggc
     2161 ggcaccggcg atgcgatctc tggtttcgaa ggcagcgtac cgaacccgta tgttaaagcg
     2221 tctgactggg gctggcagat tgatccagta ggtctgcgct atgccctttg cgaactgtat
     2281 gagcgttatc agaggccgct gtttattgtc gaaaacggtt ttggcgctta cgacaaagtg
     2341 gaagaagatg gcagcatcaa cgacgactac cgcattgact acctgcgcgc ccatatcgaa
     2401 gagatgaaaa aagcggtgac ttacgatggc gtggatctga tgggctacac accgtggggc
     2461 tgcatcgact gcgtatcgtt caccaccggg cagtacagca aacgctacgg ctttatctat
     2521 gtgaataaac atgacgacgg tgctggcgat atgtcgcgtt cacgtaagaa gagctttaac
     2581 tggtaccaag aggtgattgc cagcaacggc gagaagcttt aagtcgatga agtaccggat
     2641 gcaatacttg ttgcatccgg tcagacaact tccctgtttt atttcccgcc cgccaaatcg
     2701 ataaaacttc ccgtgacgta agaggcttta tcacttagta gccagacaat ggcctgcgcg
     2761 acctcttctg cctgtccacc acgctgcatg gggatgttcg acttaacgcg atcgacgcgt
     2821 ccaggctcgc cgccgctggc gtgcatttcg gtataaataa accctggccg cacgcagtta
     2881 acgcggatcc cctgcgcggc gacttccagc gatagtccgg tggttaacgt atcaatcgcc
     2941 cctttcgatg ccgcgtaatc aacatattcc cctggcgaac ccaaccgtga ggccaccgaa
     3001 gagacattga cgatagcgcc gccactgcca ccatttttaa gcgccatgcg ttttaccgcc
     3061 tcgcggcagc agagaaaata tcccgtcacg ttggtggaaa gtactcggtt gattcgctct
     3121 gcggtaaggt tttcaacggt gcactgggta aacaagatcc cggcgttatt gaccagcgct
     3181 gctagcggtt catcgtgctg atcgattgct gtaaacatcg caacgacctg gttttcgtcg
     3241 ctgatatccg cctggagcac gaatgctttg ccaccggctt gcgttattaa gttcatcact
     3301 tcctgcgccg cgtggaggtt ttgctgataa ttaaccgcca ccgtataccc ttcttgcgcc
     3361 aacagtaatg cagttgcccg cccgatgccg cgactgccac cggtcacaag tgctataacc
     3421 atttgttttt ccgagaaaaa ttcattcgtt gacgtatctt tgagattgct aacaaactgg
     3481 ctcaatcttc aatgttggca cgcttttccc cctcgccctt tcagggagag ggccggggtg
     3541 agggtaaata ttcgcgccag tgccggcctg ttcccctcac cctctcccca aaggggcgag
     3601 gggactgtct gagcactttt gtactttgtc atctgactaa aaaggcgccg aagcgccttt
     3661 agaaaatagt cgaatcagtg aattactggt attcgctaat cggtacgcag gagcagaaca
     3721 ggttacggtc gccgtaaaca tcatccagac gtttcaccgt cggccagtat ttgtctgcca
     3781 cacctgccgg gaatactgca acctcacggc tgtacggatg cgcccactcg gcgaccagtt
     3841 cgttctgaat gtgcggcgcg ttcaccagcg ggttatcttc cagcggccag acaccggctt
     3901 taacctggtc gatttctgcg cggatagcca gcatcgcgtc gataaagcga tccagttcca
     3961 ctttgctttc agattcagtc ggttcaacca tcagcgtacc cgccaccggg aacgacatgg
     4021 tcggcgcgtg gaaaccgtag tcgatcaggc gcttggcaat atccagctcg ctgatgccgg
     4081 tttcttcttt cagcgggcga atatcgagaa tacattcgtg cgccacgcga ccgtcgcgac
     4141 cggtatacag caccgggaag gcatcctgca ggcggctggc aatatagttg gcgttgagga
     4201 ttgccacctg gcttgctttt ttcagccctt ctgcgcccat catgcggatg tacatccagc
     4261 tgattggcag gatagaggcg ctaccgaacg gtgccgcaga aactgcgccc tgacgggtta
     4321 acatgccttc gatttgcacc acgctatgac ccggtacaaa cggtgccaga tgcgctttca
     4381 caccgatcgg tcccataccc ggaccaccac cgccgtgcgg aatgcagaaa gttttatgca
     4441 ggttgaggtg cgaaacgtcc gcgccaataa agcccggcga ggtgatgcca acctgggcgt
     4501 tcatgttcgc gccatcaagg taaacctgac cgccgaactg atgcacgact tcacacactt
     4561 cacggatcgt ttcttcatac acgccgtggg tagaaggata agtcaccatg atacaggaga
     4621 ggttatcgcc cgcctgttcc gctttcgcgc gcagatcagt cagatcgatg ttgccgtttt
     4681 tatcacacgc cacaaccacc acctgcattc ctgccatatg tgcagaagcg gggttagttc
     4741 cgtgcgcaga agccgggatc aggcagatat cgcgatgccc ttcgttgcgg ctttcatgat
     4801 aatgacgaat cgccagcagg cccgcgtatt cgccctgtgc gccggagttc ggctgcatac
     4861 aaacggcgtc gtaaccggtc agtttcacca gccagtcagc cagctgcgca atcatctgct
     4921 gataaccttc agcctgttcc ggcggacaaa acgggtgcag ttcggcaaat tccggccagg
     4981 tgattgggat catctcggcg gcggcgttca gtttcatggt gcaggaaccc agcgggatca
     5041 tcgcctgatt cagcgccaga tctttacgct ccagcgagtg catatagcgc atcatttcgg
     5101 tttcgctgtg gtagcgatta aacaccggat gggtgaggat ttcgtcgtcg cgcagcatcg
     5161 caggctggat agagcggctg tcgtgagcca cgtctttgtc cagcgtgtcg atgtccaggc
     5221 cgtggttatc gcccagcagc acgttgaaaa gctgcattac gttttcacgc gtggttgttt
     5281 catcaagggt gatcccaacc gcgttcagaa tatcgctacg caggttgatt tcagccgctt
     5341 cggcacgcgt cagtacgccc gctttgtcgg ccacttccac acacaaggtg tcgaaatagt
     5401 gcgcatggcg cagtttcaga cctttttgtt gcaggcccgc cgccaggata tcggtcagac
     5461 ggtgaatgcg gttagcgata cgtttcaggc caatcgggcc gtgataaacg gcatacaggc
     5521 tggcgatgtt tgccagcagc acctgggaag tacaaatgtt ggagttcgct ttctcacggc
     5581 gaatatgttg ctcgcgagtc tgcatcgcca tgcgcagcgc ggtattgcca gctgcatctt
     5641 tcgatacacc gataatacgg cccggcattg agcgtttgta ttcatctttc gccgcaaaga
     5701 aggccgcgtg tgggccaccg tagcccatcg gcacgccgaa gcgttgcgcc gaaccaaaaa
     5761 caatatccgc gccctgttta cccggcgcag ttaacagcac cagcgccata atatcggcgg
     5821 cgacactgac cacaattttg cgtgatttca gttcgctaat aagcgcggtg tagtcgtgga
     5881 tttcaccggt tgtgccaacc tgctgtaaca gcacgccgaa gacgtcctga tggtcgagca
     5941 ctttttgcgc gtcatcgaca atcacttcaa aaccaaaggt ttcggcacga gtacggacca
     6001 catccagcgt ttgcggatgc acatcggaag ccacgaagaa gcggttggca tttttcagtt
     6061 tgctgacgcg tttcgccatc gccattgctt cggcggcagc ggtggcctcg tccagaagag
     6121 aagcagaggc catatccagt ccagtcaaat ccagcgttac ctgctggaag ttgagcagtg
     6181 cttcaaggcg gccctgggag acttcaggtt gatacggagt gtacgcggta taccagcccg
     6241 gattttccag catgttacgc aggataaccg gcggtagctg cacggcggtg taacccatgc
     6301 cgatgtaaga cgtgaagcgt ttattgcgac tggcaatagc cttgagttct gccagtgcgg
     6361 cgtattcggt cgccggtgcg ccaacctgcg gcggggtcgc aagctgaata tctttcggca
     6421 caatctggcc ggtcagcgcg tttaacgatt gtgcaccaac ggcattcagc atttcttgct
     6481 gttgcgcggc gtccggtccg atatggcgtt caataaaagc gccgctgttt tcaagctggc
     6541 ttaacgtctg tgtcatgagc gatggttcct gaaacgtgca gtgaattgtg aacctctctc
     6601 cttacgaaga gagtgagggt gaggcataaa ttttcctcac cctgctcctc tcccgcagaa
     6661 gaggaataaa gccgttactc gtcttctaac aatgcttcgt atgcggtcgc atccagcagt
     6721 gattccagtt cgctttcatc gctggctttg attttaaaga tccagccacc tgcatacggt
     6781 tcgctgttca ccagttccgg ggaatcgctc agtgcgtcgt ttaccgccac gatttcaccg
     6841 cttactggcg cataaatgtc tgacgccgct tttactgatt cggcaaccgc gcagtcatcg
     6901 cccgcgctaa ccgttgcgcc cacttccggc aggtcaacaa acaccatatc gcctaacagc
     6961 tcctgggcat gttcggttat accaacggtg taagtgccgt cggcttcttt acgcagccat
     7021 tcgtgttctt tgctgtattt cagttctgct ggtacgttgc tcatcaatca atctccaaaa
     7081 aagtaaatca cgcgacggct ttaccgttac gcacaaaaac aggtttcgtc actttaaccg
     7141 gcatttcacg gttgcgaatt tgcacaatcg ccgtttcgcc aataccttcc ggcacgcgtg
     7201 ccagcgcaat gctgtaaccc agcgtcgggg agaaagtccc gctggtgata atgccttcat
     7261 gctggttgcc ctgcgcatcg gtaaaacgta ccggcagttc attacgcagc acgccttttt
     7321 cggtcatcac cagaccaacc agtttttctg taccatgctc acgctgcact tccagggctt
     7381 cacgaccgat aaagtcacga tctgccggtt cccaggcgat agtccagccc atgttggcgg
     7441 ctaaaggaga aatagtttcg tccatctcct gaccataaag attcatgccc gcttccagac
     7501 gcagcgtgtc acgcgcgccc aagccacatg gcttaacacc cgcttccacc agcgcacgcc
     7561 agaaatcggc cgctttttca ttgggcagcg caatttcata gcccgcttca ccggtataac
     7621 cagtggtggc aataaacaga tcgcccgcct gcacgccaaa gaacggtttc atcccttcca
     7681 ccgcctgacg ctgggcgtca ttaaacagtg tggcagcttt tgcctgcgca ttcggccctt
     7741 gcacggcaat catggaaagg tcatcacgaa cggtaatttc gatgccgaaa ggttcagcgt
     7801 gttgggtaat ccaggagagg tctttttcgc gggtggcgga gttaacaacg aggcggaaga
     7861 aatcttcagt aaagtagtag acgatgaggt catctatcac accgccagag gcattcaaca
     7921 tccccgagta aagggctttg ccgcttttgg tgagcttcgc cacatcgttc gccagcagat
     7981 aacgcagaaa ctcccgggtg cggctgccgc ggagatcgac gatggtcata tgtgacacat
     8041 caaacattcc ggcatcggta cgtaccgcat gatgttcgtc gatttgcgaa ccgtaatgca
     8101 gcggcatcat ccagccgtgg aaatccacca tgcgagcgcc gcaaagcgtg tgttgttcgt
     8161 acaaaggagt ctgttgtgcc atcttgtcct cattgaataa gcggggctga caacttttca
     8221 tggtgaaatt atcaccacga aacccagcat cggagccact cccggtcccc aacgcaatcg
     8281 ttctcttttg cctgaactta ccaccgaaac agactgttaa ccataaggta aaattgatca
     8341 tcacattagc ttatggttaa aaaacgccaa aatcgcgaca gaataaaaaa ccaaaaaaca
     8401 caccagtttc tatataaaga tgatgccatt agaaagctaa tttgaataag gcaatattaa
     8461 gagctaaaaa aatgtcaaaa aacac
//
LOCUS       DAZGXZ010000189         8354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000189 DAZGXZ010000000
VERSION     DAZGXZ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.189"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            11..529
                     /gene="ydeJ"
                     /locus_tag="ACHKJF_004775"
     CDS             11..529
                     /gene="ydeJ"
                     /locus_tag="ACHKJF_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP0952008.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
     gene            complement(574..2619)
                     /gene="dcp"
                     /locus_tag="ACHKJF_004776"
     CDS             complement(574..2619)
                     /gene="dcp"
                     /locus_tag="ACHKJF_004776"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP0952009.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQATLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            2756..3502
                     /gene="ydfG"
                     /locus_tag="ACHKJF_004777"
     CDS             2756..3502
                     /gene="ydfG"
                     /locus_tag="ACHKJF_004777"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP0952010.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3591..4277
                     /gene="rspR"
                     /locus_tag="ACHKJF_004778"
                     /gene_synonym="ydfH"
     CDS             3591..4277
                     /gene="rspR"
                     /locus_tag="ACHKJF_004778"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP0952011.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISEFVRQIR
                     QENSDWFSEE"
     gene            4455..4658
                     /gene="ydfZ"
                     /locus_tag="ACHKJF_004779"
     CDS             4455..4658
                     /gene="ydfZ"
                     /locus_tag="ACHKJF_004779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP0952012.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4694..6154)
                     /gene="ydfI"
                     /locus_tag="ACHKJF_004780"
     CDS             complement(4694..6154)
                     /gene="ydfI"
                     /locus_tag="ACHKJF_004780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0952013.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(6243..7526)
                     /gene="ydfJ"
                     /locus_tag="ACHKJF_004781"
                     /pseudo
     CDS             complement(6243..7526)
                     /gene="ydfJ"
                     /locus_tag="ACHKJF_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            8130..8243
                     /gene="ynfT"
                     /locus_tag="ACHKJF_004782"
     CDS             8130..8243
                     /gene="ynfT"
                     /locus_tag="ACHKJF_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFP0952014.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT         2237 a         2120 c         1877 g         2120 t
ORIGIN      
        1 acaaaaaaca gtgaacatta ataaagataa aattgtacaa ctcgcagata ccgacactat
       61 tgaaaacctg acatccgcgt tgagtcaaag acttatcgcg gatcaattac gcttaactac
      121 cgccgaatca tgcaccggcg gtaagttggc tagcgccctg tgtgcagctg aagatacacc
      181 caaattttac ggtgcaggct ttgttacttt caccgatcag gcaaagatga aaatcctcag
      241 cgtaagccag caatctcttg aacgatattc tgcggtgagt gagaaagtgg cagcagaaat
      301 ggcaacaggt gccatagagc gtgcggatgc tgatgtcagt attgccatta ccggctacgg
      361 cggaccggag ggcggtgaag atggtacgcc agcgggtacc gtctggttcg cgtggcatat
      421 taaaggccag acctacactg cggttatgca ttttgctggc gactgcgaaa cggtattagc
      481 tttagcggtg aggtttgccc tcgtccagct gctgcaatta ctgctataac caggctggcc
      541 tggcgatatc tccaggccag ccattggtgg tgtttatatg ttcaagccac gatgttgcag
      601 catcggcata atctgaggag ccttaccgcg ccattgtcga tacaggcgtt ccagatcttc
      661 gctgttacct ctggaaagga tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc
      721 gccctgctca acaaaccact ggtaaccatc atcggccaac atttgcgtcc acagataagc
      781 gtaataacct gcagcatatc cgccaccaaa aatatgggcg aaataactgc tgcgatagcg
      841 tggcggtata gcaggaagat ccatattttc cgccaccagc gcccgcaatt caaaatcatc
      901 gacatcctgc attgcttcgt tttcttccag gcaatgccag cgcatatcga gaagtgcggc
      961 gctaagcagt tcgctcatct catacccttt gttgaacagg ctggcattac gcattttctg
     1021 ttgcagttcg tcaggcattg ctgccccgct ctgataatgc cgggcgtagc gagcgaatac
     1081 ctgcggatgc gttgcccagt gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg
     1141 cgtgttggtg ccggaaagcg tggcataacg ctggcgggca aaaaggccgt gcaacgtatg
     1201 accaaattca tggaataagg ttatgacatc atcccagagt aacaacgcag gctcaccggc
     1261 agcgggtttc tgataattgc aaacgttata aattaccgga tgcgtttcat taagcgttga
     1321 ttgctcaaca aaattgccca tccatgcacc gccgcttttt gaatcacggg cgaagaaatc
     1381 accgtaaaat aacgccagcc ccacgccatt atgatcaaaa atttcccaca cccgaacgtc
     1441 aggatggtag acaggaatat caaaacgttc gacaaactta ataccgaaga gctgattcgc
     1501 ggtccagaat acaccttcat tcaacaccgt gtttaattca aaatatggct tgagctgcgc
     1561 ctcatcaaga tcaaatttct cccgccgtac ctgttcggca taaaatgccc agtcccacgg
     1621 ctgcgcgcta aacccgccct gctgcttatc aataaccgcc tgtatggagg ctaattcatc
     1681 gctcgcacgt tgacgcgccg ctggaacaat ttcccgcata aagttgagcg ctgcttcagg
     1741 tgtttttgcc atctgatcgg cgattttcca tgcggcataa tgaggaaaac caagtaatgt
     1801 cgcctgttgt gcacggatct ccaccagacg ttgaatgata gcgcgggtat cattgccatc
     1861 atttttttcc gctcgcgtcc agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg
     1921 catttcggca agcgccggtt gttgggtggt attcagcagc ggaatcagcc atttgttatc
     1981 cagacctttc tcgcgagccg cctctgccgc cagcgcaatc tcttgctcac tcattcctgc
     2041 cagctgcgcg atatcgttca caaccagacc gccggattta tttgctgcca gtaaccgctg
     2101 gttaaactga ctggtcagtg tcgcagcttc tgtattcagt acttttaatt ttgctttatc
     2161 agcttgcgca agtttggctc cggcaaggac aaaacgttga tgaatcacct ccaccaggcg
     2221 gatggattca ctatcaagcc ccagggattc acggcgctgc cagacagcat ctacccgcgc
     2281 gaataattca ccgttcagat agatatcatt agccagttcc gccagttcag cggaaaactg
     2341 ttcatcaaga cgctgtaatt catcattggt atgcgccgca gtcatcgcaa aaaagacgct
     2401 ggtaacgcgg gtaagtaatt ctccgctttg ttccagtgcc agaatagtat tgttgaaatc
     2461 aggcgtttgc gggttaagcg cgatggcagc aatttctgcc cgcttttgct gcattccctc
     2521 atcgaatgcc gggcgatagt gatgattggc aatttgatca aaatgaggag ccagatacgg
     2581 cagtgtgctt tgcacaagga aaggattcat tgttgtcatt ttcttctcct gaacgcgagg
     2641 tgttccatag cgtaggctta ctgataacgt agtgcaatct tgcaaaccag tattacccgc
     2701 tcttaagcat cacgtgctat gttattgaca cacaaaagcg ttgaggaaca gtgagatgat
     2761 cgttttagta actggagcaa cggcaggttt tggtgaatgc attactcgtc gttttattca
     2821 acaagggcat aaagttatcg ccactggccg tcgccaggag cggttgcagg agttaaaaga
     2881 cgaactggga gataatctgt atatcgccca actggacgtt cgcaaccgcg ccgctattga
     2941 agagatgctg gcatcgcttc ctgccgagtg gtgcaatatt gatatcctgg taaataatgc
     3001 cggcttggcg ttgggcatgg agcctgcgca taaagccagc gttgaagact gggaaacgat
     3061 gattgatacc aacaacaaag gcctggtata tatgacgcgc gccgtcttac cgggtatggt
     3121 tgaacgtaat catggtcata ttattaacat tggctcaacg gcaggtagct ggccgtatgc
     3181 cggtggtaac gtttacggtg cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg
     3241 tacggatctg catggtacgg cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg
     3301 caccgagttt tccaatgtcc gctttaaagg cgatgacggt aaagcggaaa aaacctatca
     3361 aaataccgtt gcattgacgc cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct
     3421 gcctgctcac gtcaatatca ataccctgga aatgatgccg gttacccaaa gctatgccgg
     3481 actgaatgtc caccgtcagt aatttttata cccggcgtaa ctgccgggtt attgcttgtc
     3541 acaaaaaagt ggtagactca tgcagttaac tcactcacaa gcaagaacga atgaccgtcg
     3601 aaacgcaact taatcccaca cagcctgtca atcagcaaat ttatcgtatc cttcgtcgcg
     3661 acattgtcca ttgcctgatt gctccaggca caccgttgtc ggaaaaagaa gtttctgttc
     3721 gtttcaatgt gtcacgccag ccggttcgtg aagcctttat taaactggcg gaaaacggcc
     3781 tgattcaaat tcgtccacag cgtggcagct acgtcaacaa aatttccatg gcccaggtgc
     3841 gcaacggcag ttttatccgt caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca
     3901 tgattaccga aagccagtgc taccaactgg aacaaaatct tcaccagcaa cgcattgcca
     3961 ttgagcgcaa gcaactggat gatttttttg aacttgatga caacttccat caactcctga
     4021 cgcagattgc cgactgtcaa ctggcgtggg ataccattga gaacctgaaa gcgaccgttg
     4081 atcgcgtgcg ctatatgagt ttcgaccatg tttctccacc agaaatgctg ttacgccagc
     4141 atcttgatat tttctctgcc ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga
     4201 cgcaacattt gcaggaaatc agcgaattcg tgcgccagat ccgccaggaa aacagcgact
     4261 ggtttagcga agagtaattc atttactctc atcccatccg ggatgagagg cttttccccc
     4321 cgccttacgg ctcatgcatg catcaaaaaa gatgtgagct tgatcaaaaa caaaaaatat
     4381 ttcactcgac aggagtattt atattgcgcc cgttacgtgg gcttcgactg taaatcagaa
     4441 aggagaaaac acctatgacg acctacgatc gtaaccgtaa cgcaatcacc actggcagcc
     4501 gtgttatggt tagcggcacc ggtcacactg gcaagatcct gtcgattgat actgaaggtc
     4561 tgaccgctga gcaaatccgc cgcggaaaaa ccgtagttgt tgaaggttgt gaagagaaac
     4621 tggcacctct ggacctgatt cgtctcggca tgaactaagc gtgtgaatgc cgccgatggc
     4681 ggcattgctt tttttacttc acggaatatt tcgccacggt cgctttcgcg ccatgcgcta
     4741 ataaagacaa gtacgcttcc gtcacttttg cagtaaacaa gctattgtct ggcaaatcat
     4801 caccaaagat tgccttaatc gccagcaatg actggacgcg cgctttccct tcggcactac
     4861 tttgtacagc cttctgaata acaggtaaca gtgggtcact gatttctatc ggatttccct
     4921 gttcatcaac accaccgaca taacgcatcc aacccgcgac gcccagcgcc agcagatcga
     4981 acttgctgtc atgcgccaga tgccagcgaa cagaatccaa catccgctgt ggcaatttct
     5041 ggctaccatc catcgcaatc tgccaggttc gatgacgtaa cgccgggttg ctatagcgtt
     5101 caattaatcg gttagcgtaa tcttgcaaat caacgccctg cactttcagc gtcggcgctt
     5161 gttcctgcaa catcaagcca tacgccgcat aacgataatg ttcatcttcc atgcagtcat
     5221 taatgtgctg atatccagca agataaccca aatacgccag gaatgaatga ctgccgttga
     5281 gcatgcgcaa cttcatctct tcataaggca gcacatcact aaccagttcg gctcccgctt
     5341 tttcccattc tggacgtccg gcaacaaagt tatcttctat tacccactgg cggaaaggtt
     5401 cacaggcaac gcccgcagga tcgcccacac cggtaagttg ttcgattttc gccagcgtat
     5461 cctctgtcac tgcgggcaca atacggtcca ccattgttga tgggaaagtc acgttatctt
     5521 cgatccattg tgccagtttt tcatcaacgg cttgcgcgta ggaagtgaca acgtcacgca
     5581 taacatgacc gttttctggc atgttgtcac atgacatgac ggtaaatgcg ggaagtcctg
     5641 ccgctttacg gcgagccagc gcctcaacaa tcactcctgt tgctgttttc ggctggtggg
     5701 gattttgcac gtcggcagcg accatcgggt gatcgagcat taactgtccg gtcgccggag
     5761 agtggaaata ccctttttcg gtgattgtca gagagacaat cgcgatttgc ggttcacaca
     5821 tcgcagccaa cacggtttct aagccatcca tctgtacgtg caaggctttt ttaacgacgc
     5881 caacgacgcg agccgtccac gcatcggccg acatttccgc aacggtataa agattatctt
     5941 gctgttgtaa atcggcaatt tgctgttcgc cgccgattaa gttgacctca taatatcccc
     6001 agtcactgaa atgttctgta gcaagaatat cggcatacac gccctgatgc gcacggtgaa
     6061 atgcaccaaa gcctaaatga ataattcttg gggccaggtt attacgatca taaacaggga
     6121 gtgtcgcttt tgctgataac aaattatttc ccataacaat tccttaaata taaatatggc
     6181 aagctatatg ttttgttata tgaataaaaa tcccctctcc ggtaagagaa gggattgagg
     6241 atttacagac ttctggaagg ttgcgcagct cttacaacac gcggttgatc ttccgcagcg
     6301 tcttccagcg cacttaaatc acggtctttc acctctggca ttttcagcgc agagattaaa
     6361 ccaattactg aatatgccat gatcataatg gcgatcggat accaggattc cgtcatggtg
     6421 cagaaaatac ccgccaggat aggaccaaaa ccggaagcga taagaccacc aatttcttta
     6481 gaaatagcca tccgggtaaa gcggttttta cagccgaaca tttctgccat ggtaatgttt
     6541 tccagagcaa ataatcccag caccgcacag ttatgaatca caatcagtgc aaccataatg
     6601 gtgctcgggg catagctttt atctacaatg atagaaagca ttggccatgc cagcacaatc
     6661 gcggaggtat tcataataat atacgggatc cggcgaccaa ttttatcgga taaccaacca
     6721 aggaacggaa tggtcataaa gccgagaatc gaactaatca tcaatgcatc tgttggaatt
     6781 gctttgttaa acaataacgt ctgcactaaa tagcctgcaa ggaaagtctg aattaaccca
     6841 gagttacccg cctgaccaaa acgcagccct gttgccagcc agaaggattt gctctggaac
     6901 atgctaccag caggtgcagg ttttgctgtg ggttggttgc tgtcattaac cttctcaaag
     6961 accgggcttt ctttcagatt catacgtaac cagatagcaa agaccatcac gacaacgctc
     7021 gccaggaacg gtatacgcca tccccacgcc agcagttcct ctttactgag aatgaagaac
     7081 ataaaggccc agattgccgt tgcgctcaag gttccgcagt tagttcccat agctacaaat
     7141 gaggagataa ttccgcgctt accttttggt gcatattccg ccagcatcgt accggcaccg
     7201 gaaatttccg cacctgcacc caacccctga ataatacgca gcgtcaccag caagatgggg
     7261 gcaaaaacac caatctgtgc ataggtcggt aacacaccaa ttaaggtggt acagatcccc
     7321 atcatggtga tggtaataaa gagcactttt ttacgcccta ttctgtcgcc cattttgccg
     7381 aaaataaatg ctccgacaat acgcgccaca taacctgcac cgtaggttcc cattgccaaa
     7441 attaacgcca ttgccgttga tgattcagga aaaaatattt catgaaacac taacgctgcg
     7501 ccgagcgaat ataactggaa atccataatt cacaggtgtt ttttcccatc ctgtggtttc
     7561 cttggcgttt tctaggtttt ttcagatagt tgcatttttt aaaaagcatc ctaagttcga
     7621 tctcagtgtc tatctggggc ctatttctgt cccatatatg ccccaaaaaa actccccaac
     7681 agataagtag ttttttcatg gatttatgcg taaaatcaag aacggctgga aatcattcaa
     7741 tactcacact atcgaaaaat ttaccagcca accgcagcac gttcttgcat aaggcgtgtc
     7801 tgcggttttt caactattca gatacatcac tcccatcaca ttcattcctc cgcatcaaag
     7861 gcatataggc tatatcacct tgatattttt ctttttcaga taaaaactgt tatctatgta
     7921 tacttttaaa cccaatccgt gtagagtctc tgcataagat agtttgcagt tgccgcttca
     7981 gcttgcgcca taaaccgcct gatttttgct gccacctgtt agcattcctg tatacctgaa
     8041 acgacaatgt ttatctacga actttaagaa cacccaagat aaaaattgtc aactatatca
     8101 tatataacac attactaatt cgaggctata tgaacagcat actgataatc acttctctcc
     8161 ttatcatatt cagcattttt agtcatgccc taataaaatt agggattggc atatccaata
     8221 acccagacaa aaccgatgta taagtcaaca tatcctgaat cagacataca atatcgcaat
     8281 gaaaatcaat aatattttaa ggaatatctt catgaaatca aaagacaccc taaagtggtt
     8341 ccctgcgcag cttc
//
LOCUS       DAZGXZ010000190         8281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000190 DAZGXZ010000000
VERSION     DAZGXZ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.190"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..234
                     /locus_tag="ACHKJF_004783"
     CDS             <1..234
                     /locus_tag="ACHKJF_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952015.1"
                     /translation="VDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEMLPVNLT
                     QETLFSSKSHGKYALFPIFTASWRAHRIMNKGV"
     gene            457..954
                     /gene="ubiC"
                     /locus_tag="ACHKJF_004784"
     CDS             457..954
                     /gene="ubiC"
                     /locus_tag="ACHKJF_004784"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP0952016.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            967..1839
                     /gene="ubiA"
                     /locus_tag="ACHKJF_004785"
     CDS             967..1839
                     /gene="ubiA"
                     /locus_tag="ACHKJF_004785"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP0952017.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="ACHKJF_004786"
     CDS             complement(1994..4417)
                     /gene="plsB"
                     /locus_tag="ACHKJF_004786"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP0952018.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4588..4956
                     /gene="dgkA"
                     /locus_tag="ACHKJF_004787"
     CDS             4588..4956
                     /gene="dgkA"
                     /locus_tag="ACHKJF_004787"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0952019.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHLG"
     gene            5066..5674
                     /gene="lexA"
                     /locus_tag="ACHKJF_004788"
     CDS             5066..5674
                     /gene="lexA"
                     /locus_tag="ACHKJF_004788"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP0952020.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5747..7072
                     /gene="dinF"
                     /locus_tag="ACHKJF_004789"
     CDS             5747..7072
                     /gene="dinF"
                     /locus_tag="ACHKJF_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP0952021.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEILKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRTACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7188..7397
                     /gene="yjbJ"
                     /locus_tag="ACHKJF_004790"
     CDS             7188..7397
                     /gene="yjbJ"
                     /locus_tag="ACHKJF_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP0952022.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7439..7954)
                     /gene="zur"
                     /locus_tag="ACHKJF_004791"
     CDS             complement(7439..7954)
                     /gene="zur"
                     /locus_tag="ACHKJF_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP0952023.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1884 a         2009 c         2266 g         2122 t
ORIGIN      
        1 gtcgatgatg ggcctgttat ggtggtaagc tcccagcgct tacatgatat gttgaatcct
       61 acaaaagata ccaattggaa ttccacgtat atctacaaat ccagacatga gatgttgcct
      121 gttaatctta ctcaggaaac acttttcagc tccaaatctc atggtaaata tgcgcttttc
      181 cccattttta ctgcgagttg gcgagctcat cgtataatga ataagggtgt ttaagtaaag
      241 gaaaacatca ccgttcctgg catcctgggc ggtgatgccc ctatggttgc cctctccagc
      301 gcgggcatcg gcaaagcgta aggttcaaca gcgttttacc acttcatgcg attgttgcgt
      361 ttttgttgcg tattagatca cttaatttgc tttacatctc ccgtaaacac ttttctgcga
      421 tacaatgcct ttacgttatg taacggagag ttcggcatgt cacaccccgc gttaacgcaa
      481 ctgcgtgcgc tgcgctattt taaagagatc cctgccctgg aaccgcaact gctcgactgg
      541 ctgttgctgg aggattccat gacaaaacgt tttgaacagc agggaaaaac ggtaagcgtg
      601 acgatgatcc gcgaagggtt tgtcgagcag aatgaaatcc ccgaagaact gccgctgctg
      661 ccgaaagagt ctcgttactg gttacgtgaa attttgttat gtgccgatgg tgaaccgtgg
      721 cttgccggtc gtaccgtcgt tcctgtgtca acgttaagcg ggccggagct ggcgttacaa
      781 aaattgggta aaacgccgtt aggacgctat ctgttcacat catcgacatt aacccgggac
      841 tttattgaga taggccgtga tgccggactg tgggggcgac gttcccgcct gcgattaagc
      901 ggtaaaccgc tgttgctaac agaactgttt ttaccggcgt caccgttgta ctaagaggaa
      961 aaaaatatgg agtggagtct gacgcagaat aagctgctgg cgtttcatcg cttaatgcgt
     1021 acggataagc caattggcgc gttactgctg ctctggccaa cattatgggc gttgtgggtg
     1081 gcgacaccgg gcgttcctca gctctggatc ctggcggtat ttgtcgcggg tgtctggctg
     1141 atgcgcgctg ctggatgtgt ggtgaatgat tatgctgacc gaaagtttga tggtcatgtt
     1201 aagcgcacgg cgaaccgacc acttcccagc ggcgcggtaa cagagaaaga ggcgcgcgcg
     1261 ctgtttgtag tgctggtact gatttcgttt ttactggtac tgacgctgaa tacgatgacc
     1321 attctgttgt cgattgccgc tctggcgttg gcgtgggtgt acccgtttat gaagcggtat
     1381 acccatctcc cgcaagtggt gctgggcgcg gcgtttggct ggtcgattcc aatggctttt
     1441 gccgctgtga gtgagtcggt gccactgagt tgctggttaa tgttcctcgc caatattctc
     1501 tgggcggtgg cttacgacac gcagtatgcg atggttgacc gcgatgatga tgtgaagatt
     1561 ggcattaaat ccacggcaat cctgttcggc caatacgata aattgattat tggtattttg
     1621 cagattggcg tactggcact gatggcgatc atcggtgagt taaatggctt aggctgggga
     1681 tattactggt caattgtggt ggctggcgcg ctgtttgttt atcaacaaaa actgattgcc
     1741 aaccgcgagc gtgaagcctg ctttaaagca tttatgaata ataactatgt tggtctggta
     1801 ctgtttttag gactggcaat gagttactgg catttctgat gatgtaaaaa agccggatga
     1861 tcatccggct ttcttctggg ttgccttatg cgcggcgctt ctcaggccta cacaacacat
     1921 cgcaatttat tgaatttgca gattatggaa ggccggataa ggcgttttcg ccgcatccgg
     1981 caattctctc tgattaccct tcgccctgcg tcgcactctc aatcgtcaaa cgcacgtctg
     2041 atgtaatcaa ctccgccagc aactgataaa ccttcatcgt ttctgccggt tcggcgtcgc
     2101 cgctatcgct gatataccct tcatcacgca gcgtcagcac cagagaactg aacaccgcct
     2161 tgtcgaagaa ctccggcgcg ttgatgccgt gcagcacgga gagacgttgc gcgacggtgc
     2221 ggctctcttt ctccagcgta ccgcggttga tcgacgggtt ggcactcaac aaccagaagg
     2281 tgatggcata acgttgcagc gtttcgcgcg cgcctgcggc cagcagctgt agggtgcgag
     2341 aatgcgccgg gttgatatgc aactcatcat cttgcagggt aatcagcccc tgacgttgca
     2401 tctcatttgc cagcgcatca ataacgtccg gcaactcgtc gcgatcccag cgcaggaaca
     2461 gctccgcttt cagcattggg taaagcacat tgacgtgctc catcaatacg tcgcgggaga
     2521 tgtggcgatg ctgggtgaca attgccgcca tcagcgaagg cagcaccaac atatgcgcaa
     2581 tgttgttgcg atagtaggtc atcagcaccg cttgctcgcg tggcagaatg atgatgtcgc
     2641 cgatagtgtc tttctcgact tcaaacttgt tcatttgcag cgcgtgatcg ataagctcgc
     2701 tggcgctggc tgaaggaacg gtagagtccg tggagtaagg tacgttgcgc atcagatcca
     2761 ggtagcagtt gagttgctcg gttaactgct cgcgggtgag tgagcgctga cgtgatgcca
     2821 gtagcgcagt acagcacagg ttcatggcgt ttgccgcgcc tgcgttgtta atgcgtacca
     2881 tcagatcggc agcaatatta ttgaccgtcg gcgttaacca ggccggacgc accgcttcga
     2941 tgggatcgat agattcacgc cagtctggta cgtgctggtt aaggtaggtc atcaacggca
     3001 ttggttcacc gaagttgacg taaccctgac cgagattacg cagcttgctt aaaccgcgca
     3061 gcatctgcgg caggctctct ttctctttcg tcgcgccgcg cagttctttg gcgtaagtac
     3121 ccacttccat gacgtgctca taaccgatat agatcggaat cagcgtaatc ggacgcgtgc
     3181 cgccacgcag catcgcctga atggtcatcg acagcgtacc ggttttcgga tccagcaaac
     3241 gccccgtgcg ggaacgaccg ccttccacga agtactcgac ggaataacca cggctgaaca
     3301 gttcgccgag atactcacgg aaaacggtgg aataaagttt attgccttta aaagtacggc
     3361 gaataaagaa cgcccccaga cggcggaaaa tcggcccggc cggccagaag ttcaggttga
     3421 tcccggcggc gatatgcggc ggcaccagcc cctggtggta cagcacgtaa gaaagtagca
     3481 ggtagtccat gtgactgcgg tggcaaggca catacaccag ctcatgaccg tcgtgggcca
     3541 gctggcgaac gcgctcggcg ttatggacgt tgatgccctg ataaagtcgg ttccaggtga
     3601 agcccagaat gcggtcagtc aggcggatca tctcgtaaga gaaattcgcc gcaatctctt
     3661 ccatcagcgc aatcgcgttc tgctgcgctt tttcatggga gatttttttg ctgcgcgctt
     3721 catcttctac cgctttggca atggcgcggg aggcgagcag cttattaaac agatcctgac
     3781 gagcaggcag acgtgggccc acagcagcca gacgttgacg ggcaaagtgc atacgcgcca
     3841 cgcgcgccag tttctgagcg atagttttat ctgtgccgtg ttcatcggcc atgcggcgca
     3901 gagaaactga cggcgagaaa cgcacaaaac tgtcgcgacc gagccacagt acggcgaaaa
     3961 atttttgtac gccgttaagc atacgcagcg gcgggtttac ttcgcctttt tcacgccccg
     4021 gcgcgcgacc aaacatcacc gatactggca ccatctgcac atccagattt gggttgctgc
     4081 ggtgcaaatc aagatagtcg tggaacagct taatagactc ttctttcggc gtgtaatagg
     4141 tgaacacacg cggcccgccg tgaatgaaca catagcgcgg cagtagcgtg ccgtcgattt
     4201 ccagcggctc taacgggtca ggcaagtcat gagccagaca ctgggcgcgc aacgtcagca
     4261 aatctgcttt cgagttgtac ggtaaaacgt acataattgg acgagaggta tccagcccca
     4321 gttccggggc aggatctgcc ggaatagact tgctttttac caggatgctt aatggtaaat
     4381 tcagtaattt gtagtaaatt cgtggccagc cggacataaa cgatgtaaag cctctggtta
     4441 ataatgcaaa tgcgcggcaa ggatagcaga aagtcatggg aaattctgtg gtatccgctc
     4501 atgtttcgcg cggcgttacg caaacccgaa tcatcggatt taacggtaca ctgatattga
     4561 cgctcataat gtaaaaaggt tctttcaatg gccaataata ccactggatt cacccgaatt
     4621 atcaaagctg ctggctattc ctggaaaggt ttacgcgctg catggatcaa cgaagcggca
     4681 ttccgtcagg aaggcgtagc ggtattgttg gcggtggtca tcgcctgctg gctggatgtg
     4741 gacgcaatta cccgcgtgct gcttatcagc tccgtgatgc tggtgatgat tgtggaaatc
     4801 ctcaatagcg ccatcgaagc agtggttgac cgaattggct ctgaatacca tgagctttcc
     4861 ggacgcgcaa aagatatggg atccgccgcg gtgctgattg ccattatcgt cgccgtgatt
     4921 acctggtgca tcctgttatg gtcgcatttg ggataaccct tccagatttc gataaatctc
     4981 tggtttattg tgcagtttat ggttccaaaa tcgccttttg ctgtatatac tcacagcata
     5041 actgtatata cacccagggg gcggaatgaa agcgttaacg gccaggcaac aagaggtgtt
     5101 tgatctcatc cgtgatcaca tcagccagac aggtatgccg ccgacgcgtg cggaaatcgc
     5161 gcagcgtttg gggttccgtt ccccaaacgc ggctgaagaa catctgaagg cgctggcacg
     5221 caaaggcgtt attgaaattg tttccggcgc atcacgcggg attcgtctgt tgcaggaaga
     5281 ggaagaaggg ttgccgctgg taggtcgtgt ggctgccggt gaaccgcttc tggcgcaaca
     5341 gcatattgaa ggtcattatc aggtcgatcc ttccttgttc aagccgaatg ctgatttcct
     5401 gctgcgcgtt agcgggatgt cgatgaaaga tatcggcatt atggatggtg acttgctggc
     5461 agtgcataaa actcaggatg tacgtaacgg tcaggtcgtt gtcgcacgta ttgatgacga
     5521 agttaccgtt aagcgcctga aaaaacaggg caataaagtc gaactgttgc cagaaaatag
     5581 cgagtttaaa ccaattgtcg ttgaccttcg tcagcagagc ttcaccattg aagggctggc
     5641 ggttggggtt attcgcaacg gcgactggct gtaacatatc tctgagaccg tgatgccgcc
     5701 aggcgtcgcg gtttgttttt catctctctt catcaggctt gtctgcatgg cattcctcac
     5761 ttcatctgat aaagcactct ggcatctcgc cttacccatg attttctcca atatcaccgt
     5821 tccgttgctg ggactggtcg atacggcggt aattggtcat cttgatagcc cggtttattt
     5881 gggcggcgtg gcggttggtg caacggcgac cagctttctc tttatgctgt tgctgttttt
     5941 acgcatgagc accaccgggc tgactgcgca ggcttatggt gccaaaaatc ctcaggcatt
     6001 agcccgtgcg ctggtgcaac cgttgctgtt ggcattgggg gctggggcgt taattgcgct
     6061 gctgcgtaca cctattatcg atctggcgct gcatattgtt ggcggtagtg aagcagtcct
     6121 ggaacaggcg cggcgctttc ttgaaatccg ctggttaagc gcaccggcgt cgctggcgaa
     6181 tctggtatta ctcggttggt tactcggcgt gcaatatgcc cgtgcgccag taattttgtt
     6241 agtggtcggc aatatcctca acattgtgct ggatgtctgg ctggtgatgg ggctgcatat
     6301 gaacgtgcag ggcgcggcgc tggcgacggt tattgcggaa tatgcaacat tgctgattgg
     6361 tctgctaatg gtgcgtaaaa tcctcaaact acgcggaatt tccggcgaaa tactgaaaac
     6421 tgcctggcga ggaaacttcc gtcgcttgct ggcgcttaac cgcgatatca tgctgcgttc
     6481 gctgttgttg caactctgtt tcggcgcgat caccgtactt ggtgcgcgac tggggagtga
     6541 cattatcgct gttaacgcgg ttctgatgac gctactcacc tttaccgcct atgcgctgga
     6601 tggttttgcc tacgcggttg aagcacattc cggtcaggca tacggtgcgc gcgacggtag
     6661 ccagttgctg gatgtctggc ggacagcgtg ccgccagtcg gggatcgtag cgctactgtt
     6721 ttcggtggtt tatttgctgg caggggaaca catcattgcg ttactgacgt cgttaaccca
     6781 gattcaggag ctggccgacc gctatcttat ctggcaggtg attttgccgt tggttggcgt
     6841 ctggtgttat ctgctggacg gcatgtttat aggcgcaacg cgtgccgccg aaatgcgtaa
     6901 cagtatggcg gtggccgccg caggttttgc gctgacgctc cttacgctgc cgtggctggg
     6961 taatcatggt ttgtggctgg cattaaccgt ctttctggcg ttgcgcgggc tttctctggc
     7021 ggctatctgg cggcgtcact ggcgcaacgg tacctggttt gccgcaacgt gacggttaaa
     7081 aattctgaat aaataatcct aagccaaatt gctgactaca cttaatctca cgttcagaag
     7141 aaaagtgaac gtactctcat tcacaaccta acgatgaggt cttgattatg aataaagatg
     7201 aagccggcgg taactggaaa cagtttaaag gtaaagtgaa agagcaatgg ggcaaactga
     7261 ccgatgatga tatgacgatc attgaaggta aacgtgatca actggtcggt aaaatccagg
     7321 aacgttatgg ttatcagaaa gatcaggcag aaaaagaggt cgtggattgg gaaacccgca
     7381 atgaatatcg ctggtaatta atccctcctg cccgacgtgt acaaggatgt acgccctctt
     7441 aacgcggttt ctttttcacc tgcacagagt gatcatgctg gcactgttca ggatgacgac
     7501 acgcttccac ttccacgcat gccgcacaga gcccatgtgc ttcaatcaca ttatgccgca
     7561 gggcaaaccc catttttgcc gccagcgtat gcataatgtc ttccacgcct tctgcacact
     7621 cttctttcac cgcgccgcag cgatcgcata taaacatggc tgacgtatgc gtgggctggt
     7681 cgaacagatg acagagcaca taactgttgg tggattccac cttatgcaca aaaccttgct
     7741 caagcagaaa atccagcgcg cgataaaccg ttggcggctt ggcttgcggt tcagcttcgc
     7801 gcagtaaatc aagcagatca taagcgctga tagcgccatc ctgcagactc atcaggcgca
     7861 acacttccag gcgctgtggg gtcaggcgca cattacgctg cgcgcagatt ttttcagcct
     7921 gcgctaataa ctcctgcgtt gtggtctttt ccatcgggca cctcaaagtg ggggttaaga
     7981 atgtccttac tttaccatgt tccaggaaaa acaccgagaa cccttattgt tgccgtaatg
     8041 ttgattttct gttttgtagg taaggtgttg tgttgccttg tcgtaccatt atcaacacga
     8101 taataattaa taattcattt ttaaataaat tgcttcaagt taactcgcta tttatttaac
     8161 tgggtttaaa cgcatacctg tagttcagaa attatgagcg atttttgata gtgaccagat
     8221 ggcaaaagaa ttataaggag gacacggtac gacaaaaaag gtagatgaat catgttgaag
     8281 a
//
LOCUS       DAZGXZ010000191         8200 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000191 DAZGXZ010000000
VERSION     DAZGXZ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.191"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(180..1070)
                     /gene="malG"
                     /locus_tag="ACHKJF_004792"
     CDS             complement(180..1070)
                     /gene="malG"
                     /locus_tag="ACHKJF_004792"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP0952024.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(1085..2629)
                     /gene="malF"
                     /locus_tag="ACHKJF_004793"
     CDS             complement(1085..2629)
                     /gene="malF"
                     /locus_tag="ACHKJF_004793"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP0952025.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(2783..3973)
                     /gene="malE"
                     /locus_tag="ACHKJF_004794"
     CDS             complement(2783..3973)
                     /gene="malE"
                     /locus_tag="ACHKJF_004794"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP0952026.1"
                     /translation="MKIKTGARILALSALTTMMFSTSALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            4338..5453
                     /gene="malK"
                     /locus_tag="ACHKJF_004795"
     CDS             4338..5453
                     /gene="malK"
                     /locus_tag="ACHKJF_004795"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP0952027.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            5525..6865
                     /gene="lamB"
                     /locus_tag="ACHKJF_004796"
     CDS             5525..6865
                     /gene="lamB"
                     /locus_tag="ACHKJF_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP0952028.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            7017..7937
                     /gene="malM"
                     /locus_tag="ACHKJF_004797"
     CDS             7017..7937
                     /gene="malM"
                     /locus_tag="ACHKJF_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP0952029.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         2010 a         2141 c         2126 g         1923 t
ORIGIN      
        1 taagtgggaa aacaaataac gtaaaaataa taagttctat gatgggaata ttaaccggcg
       61 aacgattcag attgcagacg aaagaaaaaa aggcgctccg tggagcgccg aataacagtc
      121 acaagttggg ataacgtaag ttgagggtgc agcggcataa cattggcaga acaacatctt
      181 taacctttca caccacctgc cgtcaggccg ttgaccagcc agcgttgagc cagcaagaag
      241 acgatggtga tcggtaatgc agacatcacg gcagcggcgg caaagtcacc ccacaggtag
      301 ttttgcgggt tgaggtattg ctgcatcccc acggccaggg tgtagctgtt tacgtcacgc
      361 agtaacagcg acgcgaccgg aacttcagta atggcagcga taaacgacag gatgaacacc
      421 accgccagaa tcggtactga cagcggcaac aggacaaggc ggaaggcctg ccacggtgtc
      481 gcaccatcca gcgccgccgc ttcttccagc gaactgtcga tggtttcgaa atagcctttg
      541 atggtccaga catgcagcgc aatcccaccc agatacgcga aaattacgcc gccgtgagta
      601 ttcaggccaa tgaatggaat gtactcaccc agacgatcaa acaacgcata caacgcgacc
      661 agtgaaagta ctgccgggaa catctggaaa atcagcattc ctttcagcag cgtcgctttg
      721 cctggaaagc gcatacgggc gaaagcgtag gcgcaggtgg tagagagcgc cacaatgcca
      781 atcgcggaaa tcccagcgac ctttaccgag ttccatagcc acagcagtac cgggaatggc
      841 ggtggcgtaa tgcgaccatc agcctgttcc acgctaaaac ctaacgccag tttccagtga
      901 tcccaggaga tttgctccgg gatcaggctg ccggtcgcaa agttcccctg gcgcagtgag
      961 atagcgacga ccatcagcag cgggaacata atcgctgcga taaaaagtag cagtagcagg
     1021 tgagtaataa acaaacgtgc tttttgcgat ttcggttgga ccattgccat ttttgttatc
     1081 tcccttaatc aaacttcatt cgcgtggctt tcaggttcac tatcgccagc gcacccacca
     1141 gcaggaagat cagcgtggca attgctgccg ccagaccgaa gtcctgaccc ccgccgcctt
     1201 caaaagcgat gcggtaggtg tagttaacaa gcaggtcggt ataaccggct ggcgtggtcg
     1261 tgccaagacg atccgggccg ccgttggtta acagttgaat cagcacgaag ttgttaaagt
     1321 taaaggcgaa gctggcgatc atcagcggcg tcagcggttt aatcagcagc ggcagcgtaa
     1381 tcttaaagaa gttctggaac ggacctgcac catccattgc tgaggcttca tacaaatcgt
     1441 ccggaatcgc tttcagcaag cccatgcaga ggatcatcat gtacggataa cccagccagg
     1501 tattgacgat aatcagcata gtgcgggcgg tggtcggatc gctgaaccag gcgggcttca
     1561 cgccaaacag cgcgctcaac atcatgttga tttcaccgaa gctctggtta aacaaccctt
     1621 tgaaaatcaa gattgaaatg aatgatggca ccgcgtaggg cagaatcagc aggacgcgat
     1681 ataccgcttt gccacgcaac gcttcccact gcaccagaca cgccagaacc atgccgaccg
     1741 ccaccgttaa aaagacggtg atcagtgaaa acaccacggt ccagacgaaa atggcgagga
     1801 acggtttctg aatgccttcg tcggtgaaga cgcgggtaaa gtttttccag ccggtggtca
     1861 cggtgtaacc ggggcttagc ttttcatccc cccagttgcc gtcggcggta atggactggt
     1921 aaaagccaat ttggttattc ggacgatatt tcacgccgct ctgattattc gtcaacgtgc
     1981 cgtcaccgtc gagtgtgtag agcggctgcg tgccagaaaa ctggcgcagg gagctcatca
     2041 tcactttgtt gccatccggc agaatggcgg taatgtcact cagcgcctga cggttctggg
     2101 taatcacgcg cagattcgcg cgttcgcctt cgggctgggc ggtcgtttct ttcagttgca
     2161 gtttttgctc gccgccaaat ttaaacgcgt cggagaggta atttttgccg gtttcgccgt
     2221 cgctgagcgc cagttgccac tcatcgcccg ccgggtaaag accaaagtta taggttttgc
     2281 ctgcttgcca ggagcgatct aacaacactt cctgcgcacg ttcaaaagtc agctggttag
     2341 tgctgctgta gttggtgaag gcaatggcga tggtgcagac cagagggaag aggacgaata
     2401 atcccattcc agccattccc gggtaaacat agcgccaggc gtaggcttta cgattggcga
     2461 aaatatacag ccccgctgaa ctcaatatca gcgtggtaat ggcgaacagg tattcccctt
     2521 gtgcgtacat taaaacaaca aggtaaccca ccagcaggcc gagcagacct agcactgacc
     2581 atttcagcgc gtcgctttgc caccaatgtt tctttttaat gacatccatg gggttcttcc
     2641 tcattccagg acggataagg ctttcacgcc ttatccgaca acaactgcct gatgcgacac
     2701 tgatgcgtct tatcaggcct acatacgttt caatttcgta ggccggacaa ggcgttcacg
     2761 ccgcatccgg catttcacag cattacttgg tgatacgagt ctgcgcgtct ttcagggctt
     2821 catcgacagt ctgacgaccg ctggcggcgt tgatcaccgc agtacgcacg gcataccaga
     2881 aagcggacat ctgcgggatg ttcggcatga tttcaccttt ctgggcgttt tccattgtgg
     2941 cggcaatacg tggatctttc gccaactctt cctcgtaaga cttcagcgct acggcaccca
     3001 gcggtttgtc tttattgacc gcttccagac cttcgtcagt cagcagatag ttttcgagga
     3061 actctttcgc cagctctttg ttcggactgg cggcgttaat acctgcgctc agcacgccaa
     3121 cgaacggttt agatggttga cccttgaagg tcggcagtac cgttacacca taattcactt
     3181 tgctggtgtc gatgttggac catgcccacg ggccgttgat ggtcatcgct gtttcgcctt
     3241 tattaaaggc agcttctgcg atggagtaat cggtgtctgc attcatgtgt ttgtttttaa
     3301 tcaggtcaac caggaaggtc agacccgctt tcgcgccagc gttatccacg cccacgtctt
     3361 taatgtcgta cttgccgttt tcatacttga acgcataacc cccgtcagca gcaatcagcg
     3421 gccaggtgaa gtacggttct tgcaggttga acatcagcgc gctcttacct ttcgctttca
     3481 gttctttatc cagcgccggg atctcttccc aggtttttgg tgggttcggt agcagatctt
     3541 tgttataaat cagcgataac gcttcaacag cgatcgggta agcaatcagc ttgccgttgt
     3601 aacgtacggc atcccaggta aacggataca gcttgtcctg gaacgctttg tccggggtga
     3661 tttcagccaa caggccagat tgagcgtagc caccaaagcg gtcgtgcgcc cagaagataa
     3721 tgtcagggcc atcgccagtt gccgcaacct gtgggaattt ctcttccagt ttatccggat
     3781 gctcaacggt gactttaatt ccggtatctt tctcgaattt cttaccgact tcagcgagac
     3841 cgttatagcc tttatcgccg ttaatccaga ttaccagttt accttcttcg attttggcga
     3901 gagccgaggt ggaaaacatc atcgtcgtta atgcggataa tgcgaggatg cgtgcacctg
     3961 tttttatttt cataatctat ggtccttgtt ggtgaagtgc tcgtgaaaac acctaaacgg
     4021 actctagttt ctttatacgg caacctcttt ccatcctcct tgcccctacg ccccaccgtt
     4081 gctttgtgtg atctctgtta cagaattggc ggtaatgtgg agatgcgcac ataaaatcgc
     4141 cacgattttt gcaagcaaca tcacgaaatt ccttacatga cctcggttta gttcacagaa
     4201 gccgtgttct catcctcccg cctcctcccc cataaaaaag ccagggggtg gaggatttaa
     4261 gccatctcct gatgacgcat agtcagccca tcatgaatgt tgctgtcgat gacaggttgt
     4321 tacaaaggga gaagggcatg gcgagcgtac agctgcaaaa tgtaacgaaa gcctggggcg
     4381 aggtcgtggt atcgaaagat atcaatctcg atatccatga aggtgaattc gtggtgtttg
     4441 tcggaccgtc tggctgcggt aaatcgactt tactgcgcat gattgccggg cttgagacga
     4501 tcaccagcgg cgacctgttc atcggtgaga aacggatgaa tgacactccg ccagcagaac
     4561 gcggcgttgg tatggtgttt cagtcttacg cgctctatcc ccacctgtca gtagcagaaa
     4621 acatgtcatt tggcctgaaa ctggctggcg caaaaaaaga ggtgattaac caacgcgtta
     4681 accaggtggc ggaagtgctg caactggcgc atttgctgga tcgcaaaccg aaagcgctct
     4741 ctggcggtca gcgtcagcgt gtggcgattg gccgtacgct ggtggccgag ccaagcgtat
     4801 ttttgctcga tgaaccgctc tccaacctcg atgctgcact gcgtgtgcaa atgcgtatcg
     4861 aaatctcccg tctgcataaa cgcctgggcc gcacaatgat ttacgtcacc cacgatcagg
     4921 tcgaagcgat gacgctggcc gacaaaatcg tggtgctgga cgccggtcgc gtggcgcagg
     4981 ttgggaaacc gctggagctg taccactatc cggcagaccg ttttgtcgcc ggatttatcg
     5041 gttcgccaaa gatgaacttc ctgccggtaa aagtgaccgc caccgcaatc gatcaagtgc
     5101 aggtggagct gccgatgcca aatcgtcagc aagtctggct gccagttgaa agccgtgatg
     5161 tccaggttgg agccaatatg tcgctgggta ttcgcccgga acatctactg ccgagtgata
     5221 tcgctgacgt catccttgag ggtgaagttc aggtcgtcga gcaactcggc aacgaaaccc
     5281 aaatccatat ccagatccct tccattcgtc aaaacctggt gtaccgccag aacgacgtgg
     5341 tgttggtaga agaaggtgcc acattcgcta tcggcctgcc gccagagcgt tgccatctgt
     5401 tccgtgagga tggcactgca tgtcgtcgac tgcataagga gccgggcgtt taagcacccc
     5461 acaaaacaca caaagcctgt cacaggtgat gtgaaaaaag aaaagcaatg actcaggaga
     5521 tagaatgatg attactctgc gcaaacttcc tctggcggtt gccgtcgcag cgggcgtaat
     5581 gtctgctcag gcaatggctg ttgatttcca cggctatgca cgttccggta ttggctggac
     5641 aggtagcggc ggtgaacaac agtgtttcca gactaccggt gctcaaagta aataccgtct
     5701 tggcaacgaa tgtgaaactt atgctgaatt aaaattgggt caggaagtgt ggaaagaggg
     5761 cgataagagc ttctatttcg acactaacgt ggcctattcc gtcgcgcaac agaatgactg
     5821 ggaagctacc gatccggcct tccgtgaagc aaacgtgcag ggtaaaaacc tgatcgaatg
     5881 gctgccaggt tccaccatct gggcaggtaa gcgcttctac caacgtcatg acgttcatat
     5941 gatcgacttc tactactggg atatttctgg tcctggtgcc ggtctggaaa acatcgatgt
     6001 tggcttcggt aaactctctc tggcagcaac ccgctcctct gaagctggtg gttcttcctc
     6061 ttttgccagc aacaatattt atgactatac caacgaaacc gcgaacgacg ttttcgatgt
     6121 gcgtttagcg cagatggaaa tcaacccggg cggcacatta gaactgggtg tcgactacgg
     6181 tcgtgccaac ctgcgtgata actatcgtct ggttgatggc gcatcgaaag acggctggtt
     6241 attcactgct gaacatactc agagtgtcct gaagggcttt aacaagtttg ttgttcagta
     6301 cgctactgac tcgatgacct cgcagggtaa aggtctgtcg cagggttctg gcgtcgcgtt
     6361 tgataacgaa aaatttgcct acaatatcaa caacaacggt cacatgctgc gtatcctcga
     6421 ccacggtgcg atctccatgg gcgacaactg ggacatgatg tacgtgggta tgtaccagga
     6481 tatcaactgg gataacgaca acggcaccaa gtggtggacc gttggtattc gcccgatgta
     6541 caagtggacg ccaatcatga gcaccgtgat ggaaatcggc tacgacaacg tcgaatccca
     6601 gcgcaccggc gacaagaaca atcagtacaa aattaccctc gcacaacaat ggcaggctgg
     6661 cgacagcatc tggtcacgcc cggctattcg tgtcttcgca acctacgcca agtgggatga
     6721 gaaatggggt tatgactaca acggcgatag caaggttaac ccgaactacg gcaaagccgt
     6781 tcctgctgat ttcaacggcg gcagcttcgg tcgtggcgac agcgacgagt ggaccttcgg
     6841 tgcccagatg gaaatctggt ggtaatagca aaacctgggc cggataaggc gtttacgccg
     6901 catccggcat aaaaacaggt tgtcattatc tgaaaggggc gaaagcccct ctgattatcg
     6961 ggtttagcgc gctattgcct ggctaccgct gagctccaga ttttgaggtg aaaacaatga
     7021 aaatgaataa aagtctcatc gccctctgtt tatcagcagg gttactggca agcgcgcctg
     7081 gaatcagcct tgccgatgtt aactacgtac cgcaaaacac cagcgacgcg ccagccattc
     7141 catctgctgc gctgcaacaa ctcacctgga caccggtcga tcaatctaaa acccagacca
     7201 cccaactggc gaccggcggc caacaactga acgttcccgg catcagtggt ccggttgctg
     7261 cgtacagcgt cccggcaaac attggcgaac tgaccctgac gctgaccagc gaagtgaaca
     7321 aacaaaccag cgtttttgcg ccgaacgtgc tgattcttga tcagaacatg accccatcag
     7381 ccttcttccc cagcagttat ttcacctacc aggaaccagg cgtgatgagt gcagatcggc
     7441 tggaaggcgt tatgcgcctg acaccggcgt tggggcagca aaaactttat gttctggtct
     7501 ttaccacgga aaaagatctc cagcagacga cccaactgct cgacccggct aaagcctatg
     7561 ccaagggcgt cggtaactcg atcccggata tccccgatcc ggttgctcgt cataccaccg
     7621 atggcttact gaaactgaaa gtgaaaacga actccagctc cagcgtgttg gtaggacctt
     7681 tatttggttc ttccgctcca gctccggtta cggtaggtaa cacggcggca ccagctgtgg
     7741 ctgcacccgc tccggcaccg gtgaagaaaa gcgagccgat gctcaacgac acggaaagtt
     7801 attttaatac cgcgatcaaa aacgctgtcg cgaaaggtga tgttgataag gcgttaaaac
     7861 tgcttgatga agctgaacgc ctgggatcga catctgcccg ttccaccttt atcagcagtg
     7921 taaaaggcaa ggggtaatta cgccccacag tgctgatttt gcaacaactg gtgcgtctcc
     7981 tggcgcacct ttttttatgc ttccttgctg ggatatgagc gattttttat agtaactcac
     8041 ttcttcttca ctaagaatat ccattatctc aatgccttat cagagattct tttcctttcg
     8101 ccggttatgt ctggacattc aggctacttt tccaggttat tttatttctg ttatgcagag
     8161 gttttatgat aagtcatatc ctaaattctg gcggcaataa
//
LOCUS       DAZGXZ010000192         8171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000192 DAZGXZ010000000
VERSION     DAZGXZ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.192"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..899)
                     /locus_tag="ACHKJF_004798"
     CDS             complement(<1..899)
                     /locus_tag="ACHKJF_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP0952030.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGL"
     gene            1235..2437
                     /gene="nupC"
                     /locus_tag="ACHKJF_004799"
     CDS             1235..2437
                     /gene="nupC"
                     /locus_tag="ACHKJF_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP0952031.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2487..4676)
                     /locus_tag="ACHKJF_004800"
     CDS             complement(2487..4676)
                     /locus_tag="ACHKJF_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0952032.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQSVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(4884..4959)
                     /locus_tag="ACHKJF_004801"
     tRNA            complement(4884..4959)
                     /locus_tag="ACHKJF_004801"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4924..4926),aa:Ala,seq:ggc)
     gene            complement(4999..5074)
                     /locus_tag="ACHKJF_004802"
     tRNA            complement(4999..5074)
                     /locus_tag="ACHKJF_004802"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5039..5041),aa:Ala,seq:ggc)
     gene            5295..5654
                     /locus_tag="ACHKJF_004803"
     CDS             5295..5654
                     /locus_tag="ACHKJF_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0952033.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            5656..6048
                     /locus_tag="ACHKJF_004804"
     CDS             5656..6048
                     /locus_tag="ACHKJF_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0952034.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6100..7515)
                     /gene="gltX"
                     /locus_tag="ACHKJF_004805"
     CDS             complement(6100..7515)
                     /gene="gltX"
                     /locus_tag="ACHKJF_004805"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP0952035.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            7774..7849
                     /locus_tag="ACHKJF_004806"
     tRNA            7774..7849
                     /locus_tag="ACHKJF_004806"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7807..7809,aa:Val,seq:tac)
     gene            7894..7969
                     /locus_tag="ACHKJF_004807"
     tRNA            7894..7969
                     /locus_tag="ACHKJF_004807"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7927..7929,aa:Val,seq:tac)
     gene            8016..8091
                     /locus_tag="ACHKJF_004808"
     tRNA            8016..8091
                     /locus_tag="ACHKJF_004808"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Val,seq:tac)
     gene            8096..8171
                     /locus_tag="ACHKJF_004809"
     tRNA            8096..8171
                     /locus_tag="ACHKJF_004809"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8129..8131,aa:Lys,seq:ttt)
BASE COUNT         2157 a         2098 c         1937 g         1979 t
ORIGIN      
        1 agtccggcag caaccagact taacccaaag accgttgccg cagcatggct taacagcggt
       61 tgcagcgtca gataagcctc atcaagatcg gcaacaccag tatgaccaga aaagtggaac
      121 gccgccgcag ctgtagccat catcgccaga ttgacaaaac cggcaatcgt catggcgata
      181 gccacatccc atttggtggc ggaataacgt tgttgacgcg aaccgccatg taaatgctga
      241 gtgagcgaag agtgcaaata aatcacatgc ggcataatcg tcgcccctaa cacgcctgct
      301 gccaggaaga ccgcttccga agtaggtaaa ctcgggatca ccattccttt acccagctgc
      361 gccaggttag gctgggaaaa aatcaactcg acaatgtaag ccgcggcaac aaacaacagt
      421 aatccaccaa tcactttttc cagcggtttt tgcccgcgac gttgcagcat taaaatcagg
      481 aaagtcgcga tccccgtcag caccgcgccc tgcaacaacg agacaccaag aatgagttta
      541 aaaccgatcg ccgcaccaat aaattccgcc aggtcggttg ccatcgcaat aatttctgcc
      601 tgaacccaat agaaccacac tacgggacgc ggatagtgat cgcgaatctg ctccgccaga
      661 tttttaccgg tggcaatccc tagtttggca gagaggatct gaatcagcat cgccatcagg
      721 ttggcccaaa cgacaaccca cagtagctga tagccgaagc tggcacccgc ctgaatattg
      781 gtcgcaaagt taccgggatc gatataacca atcgccgcaa tgaacgcagg tcccattaat
      841 gcgagcctca tcttgcgcgc cgcccgtccg ctgctactct caacgcgata gttcgtcatc
      901 ttgtgcctct aaaacatagc ctttgctatg tttcatgcta tgccaaacga gaatgattat
      961 caaattcatt taaatgggtt gtggtgattt ctctgataga cctggattat gactacgaaa
     1021 agattgatga cttgaatgtt atggttatgt ttaatgttag cacatttaca taactttcag
     1081 cttccataca caacatagca gaaatgtatg acagatcact atttttgaag cttgtcacag
     1141 gacgtcatta tagtgtgtgt cagatctcgt tttccttaac catgttacat agaatgtgca
     1201 cggaaattta acctgcctca tatttggagc aaatatggac cgcgtccttc attttgtact
     1261 ggcacttgcc gttgttgcga ttctcgcact gctggtaagc agcgaccgca aaaaaattcg
     1321 tatccgttat gttattcaac tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa
     1381 ctccgacgtt ggtttaggct tcgtgaaagg cttctccgaa atgttcgaaa aactgctcgg
     1441 atttgccaac gaagggacta acttcgtctt tggtagcatg aatgatcaag gcctggcatt
     1501 cttcttcctg aaagtgctgt gcccaatcgt ctttatctct gcactgatcg gtattctcca
     1561 gcacattcgc gtgttgccgg tgattatccg cgcaattggt ttcctgctct ccaaagtcaa
     1621 cggcatgggc aaactggaat cctttaacgc cgtcagctcc ctgattctgg gtcagtctga
     1681 aaactttatt gcctataaag atatcctcgg caaaatctcc cgcaatcgta tgtacaccat
     1741 ggcagcaacg gcgatgtcca ccgtatctat gtccatcgtt ggtgcataca tgaccatgct
     1801 ggagccgaaa tatgtcgttg cggcgctggt actgaacatg ttcagcacct ttatcgtgct
     1861 gtcgctgatc aacccttacc gtgttgatgc cagtgaagaa aacattcaga tgtccaacct
     1921 gcacgaaggt cagagcttct tcgaaatgct gggtgaatac attctggcag gtttcaaggt
     1981 tgccattatc gttgccgcga tgctgatcgg ctttatcgcc ctgatcgccg cactgaacgc
     2041 actgtttgct actgtgactg gctggtttgg ctacagcatc tccttccagg gcatcctggg
     2101 ctacatcttc tatccgattg catgggtgat gggtgttcct tccagtgaag cactgcaagt
     2161 gggcagtatc atggcgacca aactggtttc caacgagttc gttgcgatga tggatctgca
     2221 gaaaattgct tccacgctct ctccgcgtgc tgaaggcatc atctctgtgt tcctggtttc
     2281 cttcgctaac ttctcttcaa tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga
     2341 gcaaggtaac gtggtttctc gcttcggtct gaaactggtt tacggctcta ccctggtgag
     2401 tgtgctgtct gcgtcaatcg cagcactggt gctgtaaaac catacataaa aaagccgggg
     2461 atcattcccc ggctttttta cgcctgttaa tcagctaatg gctgcgggcg accaatcaaa
     2521 tacccttgca gatattgcac cccgagctta tgcaatagcg cctgctgctg ttgcgtctcg
     2581 acaaactccg cgaccacact caatgacttc gctttcgcca gatcggtaat tgatctcaca
     2641 atcatcgcat ccagcgtgtt cgtgacaata tctttcacaa agacgccatc aattttgatg
     2701 atatcagcct gcaaacgctt taaccgttcg tagttggcat atccggtgcc aaagtcatca
     2761 atcgcaatcc ggaaaccaaa cttatgcagc tgctcgatgt tgtacatgct gctttctgcg
     2821 ttagaaaacg cctgctcctc ggtgatctca agaatgaccg actgcgggga gatgtgataa
     2881 cgcttaaaca gacgaataat ccgcccggca atattctttt gcagcagcgt gagcggcatt
     2941 aaattgactg aaaagcgcgg tccttttttg tcgcaagggt gtgttgccag ccacttcaac
     3001 aaggattcca gcacttgcaa atcaaaacgc gcgctaaggt taaactgagc aataaggggc
     3061 agaaacttat ccggggtcat aatgccgccg tcatatttca gtcgcgcgag gatctcatca
     3121 taaccttcac cctctttgtt gcgaattggc tgggcgtaga gcagcaaatc accctgatct
     3181 aacgccgtgc gaatggtatt cagcaatagc acctgtttag tggtctgccc ggaaaccatc
     3241 tcctctctgc tatccagcgc cagcacatga tgatgtgcgc aggattgctc cgccagccag
     3301 cttaactgcc ccaacaaggg ttgcagggtt tcctgatttc catcaaaacg cccccaggca
     3361 gcaccatagc ccatatccag cccggtattg ttccagtgaa tttgccgact attcaggatg
     3421 ttaaccatat gctggagtcg cccttccgtt tccggcccgc tcagcactaa cagcagttca
     3481 ctacccggca attgatacaa cttttcgttt tcctgcatca acggcagcag cgtacggcaa
     3541 attgagcgga tacagtgaac gcgcatcatt aagccgtaat gacgactcat aaactcaaga
     3601 ttatcaatgc gcaggcagca aaaactcttg cccgcctctt gttccggcgc ttgttccaac
     3661 gcacgaaagt tgggtaggag cgttaacgga tctgtcagcg cctgcaaatg ccagcggcga
     3721 ttaagccatt cactgcgatg ataaatgcgc accatataga gcaggcaaac gctaaaggaa
     3781 atcagcaccg caagaataaa tgccagcgaa tattcggttt ccaccccttg caaaaagttc
     3841 tggttgtaat tcagcaggca aagcgttgaa acagcccagg tgagatttaa aaacggatag
     3901 cgaagcttac cgaccccgag ggtaaagatg atgaagaaaa cgggtaccaa gtaaccggca
     3961 ataaagtcgt tttcataagg tgtgcacaac agaagtagca gcacgcttaa cgctgccagc
     4021 cagcttaagg taaatgcgcg tttctctttg cccaacgacg gagcgatatc cctgcgccac
     4081 aatatctgcg caaagtgggg acttacaatc atgcgagtga gatagtagaa aagcatgttg
     4141 taaatcagca cagcggtgaa aaggcttagc aaatcaacga ccgtgaaaat ggcatccgca
     4201 tcgccgaaaa aggtggatat ctttagcggg aaatcaaaga agctccccac aagatacatg
     4261 ctgcatttga tgccgatcgg cgtcaccaaa ccaagccaga acagacgttg ccagacatat
     4321 cggctggtca atccataacg ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca
     4381 cacaacacga caaacgtctg gctaccgagc atgaccgcag tctgtaaaaa agagagatga
     4441 aagttccaca gattggtgca aaacatcccg cataaaacgg gaacgacacc gcgccagcca
     4501 aagataaaca agattgacag cattacgcac agtggcatcc acgccagaaa gatataactg
     4561 gaatggataa tggcaagtgg cgaaataaaa cgggatagct gaataagtac cacggtgagc
     4621 gtaaacgcta gtgtgaatat cttgatattt tttatcaggt tatgctccac aaacatgagt
     4681 acactactct cactgatcgg ggttttcagg cacacaaata ataagtttat tcgcatcgcg
     4741 aagcaaacag gggccagcct gttgccgatt tttcatccaa tgatgctttc accaggcggc
     4801 gctaacccgg acgtagactg tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg
     4861 ctttgcagcg agggttggaa atttggtgga gctaagcggg atcgaaccgc tgacctcttg
     4921 catgccatgc aagcgctctc ccagctgagc tatagcccca cgatgcgttt acgtaccaaa
     4981 tttgctgggt gcaaaatttg gtggagctaa gcgggatcga accgctgacc tcttgcatgc
     5041 catgcaagcg ctctcccagc tgagctatag ccccgtcacg taaagcttgt cgagttgacg
     5101 ggcggcatca tatgaattcc gcccgaatgt gtcaacggca aattgcaacg tgtagtttca
     5161 atcgctgaaa aatcaggcaa atgaacaatt ttggaaaacc gctcgcattc agtagttatt
     5221 catgtcacgg tttcctgtaa agtggtgtta taaaatgaac tactaataga cccacataca
     5281 ttcagggaat tgttatgttc aaggagagga tgacaccaga tgaacttgcc agactgaccg
     5341 gctatagccg ccagaccatt aataaatggg tacgcaagga aggctggaca acgtcaccaa
     5401 aacctggcgt ccagggtggc aaagcccgac tggttcacgt caatgaacag gttcgtgaat
     5461 atattcgcaa tgctgaacgt ccagaaggtc agggagaagc gcctgccctt tctggtgatg
     5521 cgccgcttga agtattgctg gtgacactgg cgaaagagat gacgccagtt gaacaaaaac
     5581 agtttacatc cttgcttctg cgggaaggga ttatcggatt gttacaacgc ttagggattc
     5641 gcgatagcaa ataatatgaa aagattacgc aacaaaatga ccaccgaaga actggctgaa
     5701 tgcctcggtg tggctaaaca aactgttaac cgttggatca gagaaaaagg ctggaaaacg
     5761 gaaaaatttc ccggcgtgaa aggcggtcgt gccagactta ttctggtcga tacgcaagtt
     5821 tgcgagttta ttcagaacac gccagccttc cataacacac caatgttgat ggaagctgaa
     5881 gagagaattg ctgaatacgc tcctggagca cgcgccccgg cgtatcgaca aatcatcaac
     5941 gcgatagata atatgacaga cattgagcag gaaaaagtcg cgcaattttt gtcacgtgaa
     6001 ggaattcgca atttcctcgc ccgtctcgat attgacgaat cagcataaat aaaaaacggc
     6061 aggatattat ctcctgccgt ttatcttttt acacgctaat tactgctgat tttcgcgttc
     6121 agcaataaaa tccagcgctt tgttgatacg ctcgatactg cgggtcttac caattgcgtg
     6181 aacggtaaca tccagtgctg gagactgccc cgcaccggtt acggcgacac gcagcggcat
     6241 accaacttta cccataccca cttccagctc atccgccgtc gcctgaatag cgtgatgaac
     6301 gttttcagcg gtccagtcag taatcgcggc cagtttgtca cgaaccactt ccagcggctg
     6361 acgcgctacc ggacgcagat gttttttcgc cgcatcggca tcgaactcag caaaatcttc
     6421 gtagaaataa cggcagctct gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag
     6481 tttcaccaga tcagccagct gcgggccgtt acgggtatcg atattttcct gctcaatgtg
     6541 ccactgtaag tgagtagcaa catactccgg cggcagcgcg ttaatgtagt gatggttcag
     6601 ccacagcagc ttgtcggtgt tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa
     6661 gtatttgatc atctcttcac gagtgaagat ttcctgatcg ccgtgggacc agcccagacg
     6721 caccagatag ttcagcagtg cttctggcaa ataaccgtca tcacgatact gcattacgct
     6781 gactgccccg tgacgtttgg acagtttttt accgtcatcg ccattgatca tagaaacgtg
     6841 cgcgtaaacc ggcaccggcg ctttcagggc cttaagaatg ttgatctggc gcggcgtgtt
     6901 gttgatatgg tcttcgccac ggataacgtg ggtgatttcc atatcccagt catcgacaac
     6961 cacacagaag ttataggttg gggaaccatc ggtacggcgg ataatcagat catccagctc
     7021 ctggttgctg aactcgatcg gaccacggat ctgatcgtca aaaacaacag aaccttcctg
     7081 cggattagca aaacgcacga cacacggttc atcatcagca tgatgctcat ggctgtggcg
     7141 gcagcgaccg tcataacgcg gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc
     7201 caggcgctct ttagagcaat agcatttata agcagtgccc tcttccagca tctgatcgat
     7261 caccgcattg tagcgatcaa aacgtttggt ctggtagtac ggaccttcat cccactccag
     7321 gctcagccag ttcatgccat ccataatggc ttcgatagct tccggcgtgg aacgctcaag
     7381 atcggtgtct tcaatacgca gcacgaactc accgccgtgg ttacgtgcaa aaagccagga
     7441 gtaaagagca gtacgcgcgc cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg
     7501 agttttgatt ttcatgaaat ggccttacgt ttagaaagat gccgacaacc ggcaaatcct
     7561 ggaaaaattg agtgggcgat attctatcac tcccgcctga ttcctcaatg tagttcgggt
     7621 ttctacctta cgcattattg ttttcgttga caaattgcgc aacacggtct gttttgcgat
     7681 catcttgctt aaatttacga cgaacgaaca atttctttag aaaatgcgtt gactcatttt
     7741 gaactctccc tataatgcga ctccacacag cgggggtgat tagctcagct gggagagcac
     7801 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccaa ctactttatg
     7861 tagtctccgc cgtgtagcaa gaaattgaga agtgggtgat tagctcagct gggagagcac
     7921 ctcccttaca aggagggggt cggcggttcg atcccgtcat cacccaccac tttctcgcca
     7981 gctaaatttc ttgtaaaaat gtgaagtacc taagtgggtg attagctcag ctgggagagc
     8041 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc acttcgggtc
     8101 gttagctcag ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc
     8161 acgacccacc a
//
LOCUS       DAZGXZ010000193         8162 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000193 DAZGXZ010000000
VERSION     DAZGXZ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.193"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1442)
                     /locus_tag="ACHKJF_004810"
     CDS             complement(<1..1442)
                     /locus_tag="ACHKJF_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFP0952036.1"
                     /translation="MKRLLPLLLGLAVSVAQAESNSDYRAGSDFARQIQGQGIGSIQS
                     FNPQESIPGYNANPDETQYYGGVTAGGDGGLKNNGTTQWATSETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQQINRSEFTNYTCERDTMVEEYCSRT
                     ARITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTVPVAGTVESASLSAHAGLYFL
                     NSRYDFMNTAFNVQLVTGQSGTFTIPVPSGFSVTQGQVLTGSGCTANGNCLPHGNGDR
                     MVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVETCPFNKADGVLKGTECSEPGGTKT
                     GVMEGKTWSITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGACLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAHSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDG"
     gene            complement(1439..1861)
                     /locus_tag="ACHKJF_004811"
     CDS             complement(1439..1861)
                     /locus_tag="ACHKJF_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0952037.1"
                     /translation="MALTKKQREKLRMKFGGRCAYCGCELPEKGWHADHVQAVLRKSE
                     QCMKAAEKRIFRLKSTGDVFRPEADCPENLVPACAPCNLLKTTYSLEMFRTQVSLQVE
                     RGRRSSVNFRTAERFRLIQVVNKPVVFWFEQYQRESEV"
     gene            complement(1887..2258)
                     /locus_tag="ACHKJF_004812"
     CDS             complement(1887..2258)
                     /locus_tag="ACHKJF_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952038.1"
                     /translation="MSLPAGYYRIDPDIRALVAAMNVHGFRTYASCQGHGFPVTKLPP
                     YIAFSCPVKMAALLEQRLRQDAESAIPRLTWGWSVKGTVNSDFQLCFRLQPEGPHHWY
                     HRYCRRSLRADFRTLVRLVNP"
     gene            complement(2255..2893)
                     /gene="trbC"
                     /locus_tag="ACHKJF_004813"
     CDS             complement(2255..2893)
                     /gene="trbC"
                     /locus_tag="ACHKJF_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFP0952039.1"
                     /translation="MSRKIFVLFALTTGLFISAHASENVNTPENRQFLKQQETLSQQL
                     REKPDTQLQAWAEQQVRANPLQHSDNQFLDELVRKQQAAQKDKPQQGAVYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKATAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGHDIIRGNLRVRQALEKVAATGDCRQVAQGLLDGTGDKSQ"
     gene            complement(2920..3441)
                     /locus_tag="ACHKJF_004814"
     CDS             complement(2920..3441)
                     /locus_tag="ACHKJF_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952040.1"
                     /translation="MGFFSFLRKISDKEKGVFISEENWFSLLEKVSAFDKVRQWAESN
                     GGAYYPLLECRRLTDIEDVRKSEDILNRLEFIDRRFREFISIMSEYNCNDKSKDRWEK
                     WLSEYPPEKIYQVRREGDHLSLKNYGDDITLDRDTAVATVQITVSRKCITQEEMDELY
                     ALSERLKEKCAGN"
     gene            complement(3504..3815)
                     /locus_tag="ACHKJF_004815"
     CDS             complement(3504..3815)
                     /locus_tag="ACHKJF_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952041.1"
                     /translation="MKVWVIRNKHADDFRKICLLFIRLTSNVLKLFPFAMVMCSVVWF
                     YFCPEYILSDIVNDWHDADTLEKIGLVRLYLKTSFILTVVGVVISYLSSDKFMPVKRR
                     K"
     gene            complement(3846..4838)
                     /gene="traU"
                     /locus_tag="ACHKJF_004816"
     CDS             complement(3846..4838)
                     /gene="traU"
                     /locus_tag="ACHKJF_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFP0952042.1"
                     /translation="MKRCMWLCVVLLLTGHVPAASADAACEGRFVNPITDICWSCIFP
                     LSLGSTKVSQGKVPDTANPSMPLQFCPAPPPVFKRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPEFGKTAQGTAKKEDKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNKPLDILFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMNTIGVDVAVCYEYPSPILP
                     KARWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(4835..5467)
                     /gene="traW"
                     /locus_tag="ACHKJF_004817"
     CDS             complement(4835..5467)
                     /gene="traW"
                     /locus_tag="ACHKJF_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFP0952043.1"
                     /translation="MKYRGLIALLVWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKARVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RIFARQGEVMNPLQYVPFNQTLYFINGDDPEQIEWMKRQTPPTLESKIILVQGSIPEM
                     QKALDSRVYFDQNGVICQRLGIDQVPARVSAVSGDRFLKVEFIPVGEGGK"
     gene            complement(5464..5850)
                     /gene="trbI"
                     /locus_tag="ACHKJF_004818"
     CDS             complement(5464..5850)
                     /gene="trbI"
                     /locus_tag="ACHKJF_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFP0952044.1"
                     /translation="MTTTQKTTDVTARQRSHWWWTVPGCLAMVLLNAAVSYAIVRLNT
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQTWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(5847..>8162)
                     /gene="traC"
                     /locus_tag="ACHKJF_004819"
     CDS             complement(5847..>8162)
                     /gene="traC"
                     /locus_tag="ACHKJF_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFP0952045.1"
                     /translation="LVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAETLFPLPEG
                     LNLPLTLRHYRVFISYCSPSKKKSRADILEMENLVKIIRASLQGAYITTQTVDAQAFI
                     DIVGEMINHNPDSLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTA
                     RILNFHLARNPEIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLK
                     YMDLEKKSKTSYAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETA
                     LEVEQDILNSFRKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFN
                     VANLMPLVADNPLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLI
                     QPLIRSVLDSGGFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERV
                     RDQLSVMASPNGNLDEVHEGLLLQAVRASWLTRQNRARIDDVVDFLKNARDNDQYAES
                     PTIRSRLDEMIVLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAV
                     MFSLIIYIENRMYRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYI
                     TITQNIVDFDSDKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIG
                     KFGAAKDQWFSSFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHE
                     AVWQLAWKKSGPEMASLEAWLEEHEKYRSVA"
BASE COUNT         1868 a         2382 c         1929 g         1983 t
ORIGIN      
        1 ccatccttac agcagttact gaagccggct gcggccttac ggcagaattt cgcctcgccc
       61 gtgaaagccc tgacatccac accattcagg gcggcaacat ccttaccagc agcggccagt
      121 gccgccagct gtgatactgc ctcaccaaaa tcactgcttt ttccgctatg cgccttgtcg
      181 cattcaccgt caaggcagaa gacatcacca ccacagacca tcacttttcc ggacgttctg
      241 gactcgcagg agtacgtggc atactcatgc agacaagctc cctcatcgga ataaaacgcg
      301 cactgacgtg aggccagcgt acaggccgga ttatcgacat atttctgaca ggtgccgtta
      361 tctgcggact gtgtgacata tttatcccgg tatgcccagc acgcctgtgt gatgctccag
      421 gttttacctt ccatcacacc cgttttcgta ccacccggtt ctgaacactc agtgcctttc
      481 agcacgccat ccgctttatt aaacggacaa gtctccaccc actccacccg tggaacccat
      541 tccttatcac ggaccttcat cctgagtttc agcgtaaagg tggaagcacc actgacaagc
      601 gattcataga ccatcctgtc accattcccg tgcgggaggc aattaccgtt tgcagtacag
      661 ccactaccgg tcagaacctg cccctgtgtg acagaaaacc cggagggcac aggtatggtg
      721 aaagtcccgc tctgtcctgt aaccagttgc acattaaagg ctgtgttcat aaagtcgtaa
      781 cgggagttaa ggaaatatag ccctgcatga gccgacagcg aggcactttc cactgtcccg
      841 gcaacaggta cggtaacgct gaacaccagt ttcagaccgt tcatactgaa ccggaactgt
      901 gagtgcggta tcgtcacctc acgatattca tccgtgtatt tccagtctcc tgtgatcctg
      961 gctgttctgg aacaatattc ctccaccatc gtgtcacgtt cacaggtata gtttgtgaat
     1021 tcactgcggt ttatctgctg cgcactgcac tgctgcccgg tatttcccac gatactgtca
     1081 gcccggttca ccacatccct gccggtctga ataaacggcg catccggtga cagaatgtct
     1141 ttcggcttgt tcatgaatga ctctgtgatg gttttgccgg tttcactggt tgcccattgt
     1201 gtggtgccgt tatttttcag accgccatcc ccgccggcag tcaccccacc gtaatactgc
     1261 gtctcatccg ggttcgcgtt atacccgggg atactctcct gtggattaaa actctgaata
     1321 ctgccaatac cctgcccctg aatctgacgg gcaaaatcag aacccgcacg gtaatcactg
     1381 ttgctttcag cctgtgccac actgactgcc agccccagca gcaatggcag aagacgtttc
     1441 atacctcact ctccctctga tattgctcaa accagaacac gactggtttg ttcaccacct
     1501 gaatcagccg gaaccgttct gcggtcctga aattcacgct gcttctgcgc ccccgttcaa
     1561 cctgcaggga cacctgcgtt ctgaacatct ccagcgaata tgttgttttc agcaggttgc
     1621 acggcgcaca ggcggggacg aggttttccg gacagtcggc ctccggtctg aaaacatcac
     1681 cggtggattt cagcctgaaa atgcgctttt ctgcagcttt catgcactgc tctgacttgc
     1741 ggagcaccgc ctgaacatgg tcggcatgcc atcctttctc cggcagttca cagccgcagt
     1801 acgcacaacg gccaccgaat ttcatacgca gtttttcccg ctgtttcttt gtcagcgcca
     1861 tcaatgcctc cttaacggga acagatttac ggattcacta atcggaccag cgtcctgaag
     1921 tctgcacgca gtgagcggcg acagtaccgg tgataccagt gatgagggcc ttcaggctga
     1981 agccggaagc aaagctgaaa atcactgtta acagtcccct tcaccgacca tccccatgtc
     2041 agacgtggta ttgctgattc agcatcctgc cgcagtcgct gctccagaag ggcagccatt
     2101 tttaccggac aggaaaaggc gatataaggc ggcagtttcg taacagggaa gccatgcccc
     2161 tgacaggacg catatgtacg aaatccgtga acgttcatcg cggccaccag tgcacggatg
     2221 tccgggtcaa tccggtaata gcctgcaggt aaactcattg cgatttatcc ccggtgccgt
     2281 ccagcagtcc ctgagccacc tgccggcagt ctcctgtggc ggccactttc tccagcgcct
     2341 gcctgacgcg cagatttccc cggatgatgt cgtgcccctg gctgcagaac accaccagag
     2401 ccggcacgct gcggatgccg tactgtgaaa acagcgtcgg gtcgatctga acaccatcgg
     2461 tcgcaccgtc tttcaccaga gacagcactg cttcagccgt ggctttcagg tcgttgttca
     2521 ccataccccg cagtgtggcc ggaataccgt agtgccgggt ttctcccagc atccgtttca
     2581 gcccctcttc gggtatggaa aacgacacaa aatacacggc accctgctgt ggtttatcct
     2641 tctgagcagc ctgctgttta cggaccagtt catcgagaaa ctggttatcg gaatgctgaa
     2701 ggggattcgc cctgacctgc tgttctgccc atgcctgcag ctgtgtgtcc ggcttttcac
     2761 gtaactgctg actcagtgtt tcctgctgct tcaggaactg gcggttttcg ggcgtattca
     2821 cattttccga agcatgggca gaaataaaca gtcctgtggt cagggcaaaa agtacaaata
     2881 ttttccggct catatcatct cccggcatag tgatataatt taattaccgg cgcacttttc
     2941 tttcagtctc tcagacaaag catataattc atccatttct tcctgagtaa tgcattttct
     3001 gctgacagta atctgcaccg tagccacggc ggtatctctg tccagagtaa tatcatcacc
     3061 gtaatttttt aatgacagat ggtctccctc tctccggacc tggtatattt tttccggtgg
     3121 gtattcagac aaccatttct cccacctgtc tttagattta tcattacagt tatattcaga
     3181 cataatgctg atgaactccc gaaatcttct gtcaataaac tccagtcggt tcagtatatc
     3241 ctctgatttc ctgacatctt caatatctgt aagccgacgg cactccagca gaggataata
     3301 tgccccacca ttactttcag cccattgtct gaccttatca aaagcactca ccttttccag
     3361 cagtgagaac caattctcct cactgataaa aacccctttt tctttatcac ttatctttcg
     3421 taaaaatgag aaaaatccca taacgcacct ttacttatat tgtacacatg cacacactgt
     3481 tatatttatg ataaccatca gactcatttc ctgcgcttaa cgggcataaa cttatctgaa
     3541 gaaagatatg aaataacaac gcccaccact gtaagaataa aagacgtctt cagatacagt
     3601 ctgacaagcc cgattttttc cagtgtatct gcatcatgcc agtcattaac aatgtctgag
     3661 agaatatatt cagggcaaaa ataaaaccac accacagagc acatcaccat agcaaaagga
     3721 aacagcttta atacattgct cgttaaccgg ataaacagaa gacatatttt tctgaagtca
     3781 tctgcgtgtt tatttcttat tacccacacc ttcatataat cgatcctctt attgattaac
     3841 atgacttaca ggaacacaca gttacgttta cgccacatca ggtaaccgaa gtttttctta
     3901 gtgttgggcg gattttttcc cgtctcccag cgcatcacac tgcgtccgaa cggatggcac
     3961 tgcccgctgt ccgggtacat attcaccatc tgataacgcc agcgcgcttt gggaagtatg
     4021 ggcgacggat attcataaca gacagccaca tccacgccga tggtgttcat tatcattccc
     4081 tgacggtgga gcttaaacgc catccgttca ctgaccagca gggatgactg cagcggactg
     4141 gattcattgc tcacccagcc attaaacgga tacatactgc cctgcgaacc ggcacaccag
     4201 aacaggatat ccagcggctt gttaaaggca ctggcaatgg catccgccgc acaggctccc
     4261 tgtgctatcg gatttgcaaa aatgaccgcc tccggattga ggatggtggt caggctgctg
     4321 tccgtccagg tagggtcaat ttctgacaga taggcaatat ccaggtctcc gccttccagg
     4381 caccccaacg acgtgatgat gttcagccag tacgtcagcg ggtatttgta ccagtgaacg
     4441 tgatagaacg ccccgttcac ctgtttatct tcttttttag ccgtaccctg tgccgttttc
     4501 ccgaattccg gcagactgaa ccccagatta accatgcatc ccggtgagcg ggtgacatcc
     4561 gtcagtgcca tcggctccca gtaaccaata gccagcccga tacgtttaaa aacaggggga
     4621 ggcgccgggc agaactggag cggcattgac gggtttgccg tatccggcac ctttccctgg
     4681 ctcacttttg tgctgcccaa cgagagaggg aagatgcagc tccagcagat atccgtgatc
     4741 gggttaacaa accgcccttc acaggcagcg tccgctgacg ctgcgggaac atggccagtc
     4801 agcagcaaaa ccacacacaa ccacatacac cgtttcattt ccctccctcc ccgaccggaa
     4861 taaactccac cttcaggaaa cgatcgccac tgacagcact cacccgtgcc ggcacctgat
     4921 caatgccgag acgctggcag atcacgccgt tctgatcaaa gtaaacacga ctgtccagag
     4981 ccttctgcat ctccgggata ctgccctgca ccaggataat tttgctttcc agcgtgggag
     5041 gcgtctggcg cttcatccat tcaatctgct caggatcatc accgttgatg aaatacagtg
     5101 tctggttaaa aggcacatac tgcagcgggt tcatcacctc cccctggcgg gcgaagatac
     5161 gaccttcgtt atcccggata tccgcagcca gtctgaccga cgggtcaaac aggcggctgc
     5221 tgtatttctc cgtccgaccg ataccgggca cggcaggagg ccgcaggctg ttgcggatca
     5281 cccgcgcctt aaacgcatcc attttccggc ccatctctcc ggactgctcc agagctgtca
     5341 gacgctgcat aatcaccgtc agcatgtccg gctcctttac cggccacaaa tctccccagg
     5401 tgccaagatc ggctgcggcc acactctgcc cccataccag cagggcaatc agcccccggt
     5461 atttcatggt tctgccctca ttcgccgggc aatatcctgc tggatttcac gggtgatatc
     5521 cggtgccccc tgaaccacgg caggcgacac cagaatgacc gcgtggtgct tctgctgcca
     5581 cgtctgcaga ctggcttcca gtgccgtgtt aaaacgcgct gaaagggctt ttgactgtgc
     5641 ttctgacagt tgtttctgac tggcgctgtc aaaaaacgca tccactgtct gtttcatgtt
     5701 gaaggcgacg gttaccggcg tgttcagtct gacaatggcg taactgacgg ctgcgttcag
     5761 taacaccatt gccaggcatc ccggcaccgt ccaccaccag tggctgcgtt gacgggctgt
     5821 aacatcagtc gttttttgcg ttgtggtcat gcaacactcc tgtatttctc atgttcttcc
     5881 agccaggctt ccagcgaagc catctccggc cctgacttct tccacgccag ctgccacacc
     5941 gcctcatgaa tgctcagccc ctccttacgt ttctgctgca caaactcaaa atccgggccg
     6001 tcagagctgt acatggcacg gcttaacggg tccacaaaca gacggtgcca ggaggaatgg
     6061 ttttccacct gcagcaggaa ggaactgaac cactggtctt tggccgcgcc aaacttgcca
     6121 atcatgtcac gctgcagtgg caggaactgg tccggataca gctggttgta tttcgcgaac
     6181 tccttcgcac tctgtttgag gataattttg taggaagagt taccccatgc cgcacgggcc
     6241 gcactggagg ccttgtcaga gtcaaagtcg acgatgttct gtgtgatggt gatataggca
     6301 ccggtatgac gacgggcggt acggtaacct ttctcaataa actcaccgac cttgtgattt
     6361 ttgaagtcca gcagacgcca gccttcatca ataacgttca gtttcttgag gttacgcggc
     6421 gtgcggtaca tccggttctc gatgtaaatg ataaggctga acatcaccgc aaccagcagg
     6481 gacggacggt cttccagtcc acccagctcc agcaccacca tttttgcgtc atcccgcagg
     6541 gacggttcat cagagttaaa atactggccg taagtaccgt tggcagtgta ctggtccagc
     6601 agcactatca tttcatccag acggctgcgg atggtcgggg actcggcata ctggtcgtta
     6661 tcccgggcgt ttttcaggaa atccaccaca tcatcaatac gcgcccggtt ctgtctggta
     6721 agccaggagg ccctgaccgc ctgcagcagc agaccttcat gcacttcatc caggttaccg
     6781 ttggggctgg ccatcaccga caactggtca cggacacgct ccgctgactg gtcaatatcg
     6841 gtgatgttcg caaacggatt aaagcgcagg gtttcaccgt ccagatatac gccccccatg
     6901 ttctcacaca gggacttgta gccatccccc atgtcgaaca ccacggcaaa gcccccggag
     6961 tccagcacgc tgcggataag tggctgtatc agcccggttt taccggcccc ggaggtgcca
     7021 cagaccgcca tgttgtagtt ggtgttattc atcccccgga aaaaaatatc gataaacgcc
     7081 agctggttac ggtaggtggg tgccagcaga cctgccggtg tcagggggtt atccgccact
     7141 aacggcatca ggttggcaac attaaagctc tctgcgcgtt gtaccactcc ggcctctttc
     7201 agctgtttaa acagcccttt cccggccata aagggcaggc aggtcagaaa attgcgcatg
     7261 tggttaaagc gcggtgaaat cagctcaaaa ccgtttttac ggaagctgtt caggatgtcc
     7321 tgctccactt ccagtgccgt ttcattattg tccttgcaga aggctgtgat gttgaggaag
     7381 taggacacga cagaagactg accggatccc agccgctggc gcagttcccc ccactccttc
     7441 gcctcctttt caactgacgg aaaccatttg gcataggagg tcttcgactt cttctccagg
     7501 tccatgtact tcaggttggc ttcgctgtgg gttttcacct ggtcttccac caccagggtc
     7561 agcgtcagga tgaacgggca ggaaatggac agttccgggt tcagcaggtt gctgtagttg
     7621 tcggccatgt tccacaggaa ggcgatttcc gggttacggg ccagatggaa attcaggatg
     7681 cgggcagtgc tgttcctgcc gttctcacgc agtcccagcg tcaggtaatc tgcccggacc
     7741 ttcaggtcaa aactgtcctc cacgcactga taattcaaat cagaatacgg gtccagctga
     7801 cgtcttttcg ggtacaggga gtccgggtta tggttaatca tctccccgac aatatcgatg
     7861 aaagcctgtg catccaccgt ctgcgtggtg atatatgctc cctgcagaga cgcccggatg
     7921 attttcacca ggttttccat ttccagaatg tcggcccggc tttttttctt cgagggagaa
     7981 cagtacgaga taaacacccg gtaatggcgc agggtcagag gcaggttcaa cccttccggc
     8041 agcggaaaca gtgtttctgc ggctttcata taataggccc gggtaatggc gttaaaccgt
     8101 tctgcctgtt cacctgacca ggagaactca cgcagcccgt attcaatcct gtcaccaacc
     8161 ag
//
LOCUS       DAZGXZ010000194         8149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000194 DAZGXZ010000000
VERSION     DAZGXZ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.194"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(137..868)
                     /gene="dnaQ"
                     /locus_tag="ACHKJF_004820"
     CDS             complement(137..868)
                     /gene="dnaQ"
                     /locus_tag="ACHKJF_004820"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP0952046.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            933..1400
                     /gene="rnhA"
                     /locus_tag="ACHKJF_004821"
     CDS             933..1400
                     /gene="rnhA"
                     /locus_tag="ACHKJF_004821"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP0952047.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1397..2119)
                     /locus_tag="ACHKJF_004822"
     CDS             complement(1397..2119)
                     /locus_tag="ACHKJF_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0952048.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALEHQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2153..2908
                     /gene="gloB"
                     /locus_tag="ACHKJF_004823"
     CDS             2153..2908
                     /gene="gloB"
                     /locus_tag="ACHKJF_004823"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP0952049.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            2980..4338
                     /gene="mltD"
                     /locus_tag="ACHKJF_004824"
     CDS             2980..4338
                     /gene="mltD"
                     /locus_tag="ACHKJF_004824"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP0952050.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4386..5156)
                     /locus_tag="ACHKJF_004825"
     CDS             complement(4386..5156)
                     /locus_tag="ACHKJF_004825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0952051.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5234..6034)
                     /locus_tag="ACHKJF_004826"
     CDS             complement(5234..6034)
                     /locus_tag="ACHKJF_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0952052.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6275..7189
                     /gene="yafC"
                     /locus_tag="ACHKJF_004827"
     CDS             6275..7189
                     /gene="yafC"
                     /locus_tag="ACHKJF_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP0952053.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7186..7989)
                     /gene="dkgB"
                     /locus_tag="ACHKJF_004828"
     CDS             complement(7186..7989)
                     /gene="dkgB"
                     /locus_tag="ACHKJF_004828"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP0952054.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         2163 a         1954 c         1981 g         2051 t
ORIGIN      
        1 ctccgcgttg tgttccgttg ggaacgaggc gaatattacg gattgcctca cccttcgtca
       61 acggtttttc tcatcttttg aatcgtttgc tgcaaaaatc gcccaagccg ctatttttag
      121 cgccttttac aggtatttat gcccgccaga ggcagcttcc gcccttcttc tccaccagat
      181 caagacgggc ttcatgagct gcaagctctt catctgtcgc aaaaacaacg cgtaacttac
      241 ttgcctgacg tacaatgcgc tgaattgttg cttcaccttg ttgctgctgt gtctctcctt
      301 ccatcgcaaa agccatcgac gtttgaccac cggtcatcgc cagataaact tccgcaagga
      361 tctgtgcatc gagtaatgcc ccgtgtagcg ttcgtttact gttatctatt tcgtagcggg
      421 cacataacgc atcgaggctg ttgcgcttac caggaaacat tttcctcgcc accgcaaggc
      481 tatcggtgac cttacagaag gtattggtct tcggaatatc gcgcttaagc aacgaaaact
      541 cgtagtccat aaagccgata tcgaacgctg cgttatggat caccaactcc gcgccgcgga
      601 tatagtccat gaactcatcg gctacttcgg caaacgtggg cttatcgagc aaaaattcat
      661 cggcaatacc atgtacgcca aaggcttccg gatccaccag ccgatcgggt ttgagataaa
      721 catggaagtt attgcccgtc aggcgacggt tcaccacttc aacggcacca atctcaatga
      781 tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc ggtggtttcg gtatcgagaa
      841 cgatctggcg tgtaattgca gtgctcatag cggtcattta tgtcagactt atcgttttac
      901 agttcgattc aattacagga agtctaccag agatgcttaa acaggtagaa attttcaccg
      961 atggttcgtg tctgggcaat ccaggacctg ggggttacgg cgctatttta cgctatcgcg
     1021 gacgcgagaa aacctttagc gcaggctaca cccgcaccac caacaaccgt atggagttga
     1081 tggccgctat tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg
     1141 acagccagta tgtccgccag gggatcactc agtggatcca tagctggaaa aaacgtggct
     1201 ggaaaaccgc agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt cttgatgctg
     1261 cattggggca gcatcaaatc aaatgggaat gggttaaagg ccatgccgga cacccggaaa
     1321 acgaacgctg tgatgaactg gcccgtgctg cggcaatgaa tcccacactg gaagatacag
     1381 gctatcaagt cgaagtttaa gcctgtggtt tacgatattg ccgggtggct ccaaccgcct
     1441 ggcgaattcg tggcttgttt ttactctgtt tcatcggatt tagcgttaaa ggaatagtcc
     1501 gtttccgggc aacaataagt tgtaagcaac caagcgcagg aatatgtgca ttcaatagtt
     1561 ttccaccgtg tttgttccac gggagaacgt ggaaacggct ggcgtgcagc acttcaaaat
     1621 tcaacaaaga gagccagtcc agttgccgca tcagagtaaa catccggctg ttatagggcg
     1681 aggttttgcg caataccggc acaagtttgc gtaatcccat taaactgatg ggattgaagc
     1741 cactaatcac cagccagcca tcatcaatca atacccgatc ggcttcacgc aataaacgat
     1801 gcggatcggt gcaccacggc aatgtatgtg ccagtagaca aacatcaacg gatttatcgg
     1861 caaaaggaag atgaagtggg tccgcctgta cctggacggg cattccttgc gcagaaacat
     1921 tcacttgatg agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct
     1981 taagcagatg aaaaccatac attttagtga accacgggtt gagctggtgc tccagcgcct
     2041 tgcgataaag ctctccccag ggcaaatcgc cccagcaatc aggagccacg acagtttgag
     2101 ggactcttgc cggtttcatc acaaccttcc gtttcacact aagaggtaat ctatgaatct
     2161 taacagtatt cccgcctttg atgacaatta catctgggtt ttgaatgatg aagcaggtcg
     2221 ctgcctgatt gtcgatcccg gagacgcaga gccagtatta aacgccatta ccgccaataa
     2281 ctggcaaccg gaggccatat ttctcaccca ccatcatcac gatcacgttg gcggcgtaaa
     2341 agaactggtg gaaaagtttc cgcaaattgt ggtgtatggt ccacaagaga cacaagataa
     2401 gggaacaaca caggtagtca aagatggcga aactgccttc gttttggggc atgaatttag
     2461 tgtaattgcc acaccgggtc acactttagg acatatctgt tacttcagta aaccttatct
     2521 attttgcggc gacacgctgt tttctggtgg gtgtggtagg ttgtttgaag ggacagcatc
     2581 acaaatgtat caatcactta ataagttaag tgcgttacct gacgatacat tggtatgttg
     2641 tgctcatgaa tatacgttat caaatatgaa gtttgctttg agtattcttc cgcacgattt
     2701 gtccataaat gattattatc gtaaagttaa ggagttacgg gcaaaaaatc aaataacact
     2761 acccgtaatt ctgaaaaatg agcggcaaat taatgttttt ttaagaacag aagatattga
     2821 tttaattaat gtaattaatg aagaaacatt attgcaacaa cctgaagaac gttttgcatg
     2881 gttaaggtca aagaaagata ggttctgata aaactttctt gtcatcggct ccgttcgccg
     2941 ttatgatcgg tcgtctttta agcaactatt gacacacaca tgaaggcaaa agcgatatta
     3001 ctcgcctctg tcctgctcgt gggttgccag agtaccggca acgttcaaca gcacgcacag
     3061 agcctttctg cagctggtca aggggaagca gcaaagttta caagtcaggc acgatggatg
     3121 gacgatggga cgtctatcgc gccagatggt gacttgtggg ctttcattgg cgacgagcta
     3181 aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga aacagaaata tttacgcaat
     3241 aagagctatc tccacgatgt aactttacgg gcagagccgt atatgtactg gatagccggg
     3301 caagttaaaa aacgtaacat gcctatggaa ctggtactac tacccatagt ggagagcgct
     3361 tttgatcctc acgcaacgtc tggcgccaat gccgcaggca tctggcagat cattccgagc
     3421 acggggcgca attatggttt gaaacagacc cgcaattatg acgcgcgtcg cgatgttgtt
     3481 gcttcaacaa ctgccgcgct gaacatgatg cagcgtctga acaagatgtt tgacggcgac
     3541 tggcttctga ccgtagcggc ttataacagc ggcgaaggtc gggtcatgaa ggcaattaaa
     3601 acgaacaaag cgcgtgggaa atccacggac ttctggtcgt taccgttgcc gcaggaaacg
     3661 aagcagtacg tgcctaaaat gctggcattg agtgatattc tcaaaaacag caagcgttat
     3721 ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg cgcgtgtgca cctgagcagc
     3781 ccggttgaaa tggcgaaggt tgcagatatg gcggggattt ccgtcagcaa gctgaagaca
     3841 ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg gtccgcaata cgtgatggtg
     3901 ccaaagaagc atgcagatca actgcgtgaa tctctcgctt caggcgaaat tgctgctgta
     3961 cagtcgacgc tggttgccga caatacgccg cttaacagcc gtgtttacac cgtacgctct
     4021 ggcgacacgc tttcaagtat cgcttcacgt ctcggcgtaa gcaccaaaga tttgcagcag
     4081 tggaacaaac tgcgcggatc taagctgaag ccaggccaaa gtttgacgat tggtgcaggc
     4141 agtagcgcac agcgactggc aaacaacagc gatagcatta cgtatcgtgt gcgcaaaggc
     4201 gattcgcttt caagcattgc taaacgccac ggcgtgaaca tcaaagatgt gatgcgctgg
     4261 aacagcgaca ctgcgaatct gcaaccaggc gacaagctga cgttgtttgt gaaaaacaac
     4321 aacatgccag attcctgaca aaccagataa taaaaaggca ccgattcccc cggtgccttt
     4381 tttatttatg ctgctttatg tgcttctacc atgatgatat cactggtgaa agagccatca
     4441 ttctgcaagg caaaatacgt tctcacctct gtcgatgcgc tctgttggta aatgcgaata
     4501 gcgtctacta acgcttctgg cgtacgcatt ctcgcgaccc atgaagaaaa ttccagcggt
     4561 aacttatctg taattaaatt atcaactatc agattggctt cattgattaa cctcaaccac
     4621 tcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta atgcttctac cgtctgtaac
     4681 cagatgtcgc gcactggatg acccggagac attacgtcca tcacaatcat cctaccgcca
     4741 ggtttcaata tcctattcac ttctcgcagt gctgcaccaa catcatgcca gtgatgggca
     4801 gaataacggc tgataacaat atcaaatgcg ttatcggcaa atggcagact ttcggcataa
     4861 ccctggcggg tggtgatatt tttcagttgc cgggcttcgg cagcttgtgc cacgacatcc
     4921 agcatttggg cagataagtc atacgccacc accgcgctca cgttctgcgc agcgacaaag
     4981 ctggcatgcc ctgctccgca gcccatatca agtacacttg catcaggata atcagccaga
     5041 cgcaccgcca ggcgctgtaa atctcgcccg gatgcatgta cggtactggt taaatactca
     5101 ctggcctgcg agctaaactg cttttctacg tggtcatggt gggattgtgt tgtcattgcg
     5161 ctgtcctttt gttgtttgag tataaaagga cagcgccaaa cgttggtctg ccctgtggca
     5221 gacctgacat accttattta tcaggcttgc cgggactgaa ttcaacgagt agcggattgt
     5281 gatcggaagc gcgcgtaacc aatacagaag cttcgctgac gttcagacca cggtagaaaa
     5341 caaaatcgag cgggcggcca aacgcccgac ggcgctgatc atcggtaaaa cgcacctggc
     5401 gcagcgacat ttcccgcgca aagcgatata acgcgttcat ccttctacgg ctccaggcat
     5461 tgaaatctcc tgccataatg accgggccgc tgtggtgagc tatctgatca ccaataggaa
     5521 gtaactgctt actatagaca tccacgccca gactgaagtt gacggcgtgt atattaacca
     5581 ccatcaacag gcgggtgtca ggtaatggat agaccgtcac cagtgccgac ttcgccagac
     5641 gcaaaatggg ttctcgttca cgtaacgggc agcaatacac tggatgcgcc gccgaaaggg
     5701 tcattacgcc agaaggatgt tgtggcagta cgaaagcggg tacctgatcg gcggcaagat
     5761 agttagcggt cgcaaactgt actaactctg gcgttgtctg cgcttcctgc aataacacca
     5821 gatgtgcatc tttgccgtag ttctttaata ccgacaacca ttcagcgcgt tgctgtttgt
     5881 atatgttcca caccaggatc cgaatacgct cttcggtact taacggttct ccgggtggta
     5941 atgcctggcc gatgctcgca aaagaccccg gcggtaagat cctttccgca ggttgtccgg
     6001 caacatagcg catggcatag gtgttttttc gcacttttga ctttgaaacc tctattacgt
     6061 caacccttcc gcactcggga gggttctact gttataggga ttttagctca ctctttcaac
     6121 gagcaattgt atattgttat gtaagcaagt gcttacaccg gtcagaattt tgctcattat
     6181 ggagaacatt aaatcgcatg acatatcacg catcctgatg gtttaatgac aaataaattc
     6241 aaaatatctc ttctattttg caacaggagc aaaaatgaaa gccacgtcgg aagaactcgc
     6301 catttttgtt tcggtcgtcg aaagcggcag ctttagccgg gcagcggaac aattagggca
     6361 agcaaactca gcggtaagcc gggcggtgaa aaagctggag atgaaacttg gcgttagcct
     6421 gcttaatcgg accacgcgac aacttagcct gacggaagaa ggcgagcgtt attttcgtcg
     6481 cgtacagtca attttgcagg agatggcatc ggcagaatca gaaattatgg agacgcgtaa
     6541 tacaccgcgt ggactgttac ggatcgatgc cgcaactcca gtggtgctgc actttctgat
     6601 gccgttaatt aagcctttcc gtgaacgcta tccggaagtc actttgtcgc tagtctcctc
     6661 cgaaacgatt attaatttga tcgaaagaaa agtggatgtc gcgatacgcg ctggtacgtt
     6721 aacggattcc agcttacgtg ccaggccgtt atttaacagt tatcgaaaaa ttatcgcctc
     6781 ccccgattat atttcccgct acgggaagcc agaaacgatc gacgatttaa agcaacatat
     6841 ttgcctggga ttcactgaac ccgcttccct caatacctgg ccgatagccc gtagcgatgg
     6901 acaattacat gaggtgaagt acggtttgtc atccaatagt ggggaaacac tgaaacagct
     6961 ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac atgatcgaca aagaaatcgc
     7021 tcgcggagaa ttggtggagt taatggcaga taaagtgttg ccagtggaaa tgccattcag
     7081 tgcagtctat tacagcgacc gtgcggtaag tacgcgcatc cgggctttta tcgatttcct
     7141 tagcgagcat gtaaaaacag ctcccggagg agctgtcaga gaggcttaat cccattcagg
     7201 agccagacct tccgggctaa ccaggcggtc gttgcaatcc agtgcggcga tcgctttttt
     7261 atcttcggca tcaagctgta aattttgtgc cttaagatta ctttccaggt tttcacgttt
     7321 agtagaagaa ggaattactg agtaaccttc ccccatagcc cacgccagaa tcacttgtgc
     7381 cggagtcgca ttgtgtttag ctgcgatacg agcaataacc tcatctttca gggccttacc
     7441 atacgccagc gtcatatagg aagtaatatg gatgccgtgc tgtttagccc agacaaccac
     7501 tttacggttt tgcagataag gagagagttc aatctggtta gtagcgatgt tttcagcgcc
     7561 aacagcagca atcgtttttt ccatcaacgg gatcgtgaag ttggaaatac cgatctcacg
     7621 tgtcagacct tgttttttgg cttccagcag cgcctgcata aactcttcaa cagagacttc
     7681 atcgtttggt gacggccagt ggattagcgt cagatcaaca taatcggtac gcaatttttg
     7741 caggctctct ttcagacttg ggatcaattt gtctttgctg agattttcaa tccagatttt
     7801 agtggtgatg tagagttcat gacgtggcac gccactttct gcaatcgcct gacctactgc
     7861 ggcttcgtta tcatagattt gtgcggtatc aattgcgcga taaccaagtt caagcgccgt
     7921 tttcacagat gaaataacaa cgtcgtcttt cagacggaaa gtacctaaac caaatgcagg
     7981 gatagccata atatgcctct tttaatgcta ttttcgttaa ctgaggtgat tatgggcttc
     8041 agactgttga ggaaaaagag cgtaaaatgc agaggatttt tgcgattctg gcaataatag
     8101 atatacagac gaaaaaaaac ctcgagcgtt aactcgaggc ttcttaata
//
LOCUS       DAZGXZ010000195         8088 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000195 DAZGXZ010000000
VERSION     DAZGXZ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.195"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            306..824
                     /locus_tag="ACHKJF_004829"
     CDS             306..824
                     /locus_tag="ACHKJF_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0952055.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            1034..3175
                     /locus_tag="ACHKJF_004830"
                     /pseudo
     CDS             1034..3175
                     /locus_tag="ACHKJF_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            3251..7504
                     /locus_tag="ACHKJF_004831"
     CDS             3251..7504
                     /locus_tag="ACHKJF_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFP0952056.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSA
                     SSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYG
                     ESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRLTAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATISPDGKRSTI
                     EYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYHYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSH
                     TCEGHRVSVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYTLTG
                     QLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLDIDI
                     PYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVI
                     RMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGW
                     MSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRS
                     LAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAE
                     YIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPGQ
                     QYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVN
                     GDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWNSRA
                     SLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITI
                     IESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            7516..7965
                     /locus_tag="ACHKJF_004832"
     CDS             7516..7965
                     /locus_tag="ACHKJF_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFP0952057.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT         1992 a         1952 c         2535 g         1609 t
ORIGIN      
        1 tgataattca gaaaggataa atacaatatt tattcatgat tcagctttgg ccattttatg
       61 gaggcaatac ggactttgaa cagttgggta tggttatcca gatatatatt attaagttat
      121 tgctgaataa gagatttaat gattttaaaa atagttttta cgacaaccgt aaattctagc
      181 tcggtttgta tgtaaaaaat actgaaaaat gatgcggttc cgtgaagtga gctgcatctg
      241 cctgtctggg attaacggca ggtcattgta agtagtaatt aaccacgtta acaaggagtt
      301 tacaaatgcc aacaccgtgt tatatctcca tcactggtca gacccaggga aatattactg
      361 caggtgcatt taccgctgat tctgtcggca acatctatgt gcaggggcac gaagacgaga
      421 tgctggtgca ggaattcctg cataacgtta ctgtcccgac cgatcctcag tccggtcagc
      481 ctgcgggtca gcgtgctcac aagcctttca tttttaccgt ggcgctgaac aaagcggttc
      541 cgctgatgta caacgcactg gcctccggcg aaatgctgcc aaccactgaa cttcactggt
      601 ggcgtacctc tgtggaaggc aaacaggagc attatttcac tacccgcctg actgattcga
      661 cgattgtcga tatgaaactg catatgccgc attgccagga cccggcgaaa cgcgagttca
      721 cccagttgct cgaagtttca ctggcctacc gcaaaattga atgggaacac gtcaaatctg
      781 gcacctccgg tgccgacgac tggcgcgcac cgctggaagc ataagttaag ccaacagcat
      841 ccggctatca ggccggatgc tgtttttgta ttacgccgta gaaagaaagg ttgtcagtaa
      901 ggggttacgt tcacgggaga tggcggctgc cggaaacttc ggcggtagcg tgcctgaacg
      961 caagcgtaac actgtactgt cacagccgct ttaacagcga actgtctgtt tttccttaac
     1021 cccggaggtt gctatgtcaa ccggattacg tttcacgctg gaagtggacg gcctgccacc
     1081 ggatgctttt gcggtggtct cctttcatct gaaccagtca ctctcttcgc ttttttccct
     1141 cgatctctct ctggtcagcc agcagtttct ctcccttgaa ttccagcaga tcctcgacaa
     1201 aatggcctac ctgacgatat ggcagggcga tgacgtacag cgccgggtga aaggtatggt
     1261 gacctggttt gaactggggg agaacgacaa aaaccagatg ctgtacagca tgaaggtgtg
     1321 cccaccgctg tggcgcacag ggctgcgcca gaacttccgt atcttccaga atgaggacat
     1381 cgaaagcatc ctcggcacga tattgcagga aaacggggtg accgagtgga gcccgctgtt
     1441 cagcgagcca catccttccc gtgagttttg tgtccagtac ggtgagactg attacgattt
     1501 cctgtgccgg atggcggcgg aggaaggcat cttcttttat gaggagcacg cgcaaaaaag
     1561 taccgaccag agcctggtcc tgtgcgatac cgtgcgttat ctgccggagt cctttgagat
     1621 cccctggaat ccgaacaccc gtaccgaggt gagcaccctc tgcatcagcc agtttcgcta
     1681 cagcgcacaa atccgccctt cttccgtggt gaccaaagac tacaccttta aacgacccgg
     1741 ctgggcaggg cgttttgatc aggaaggcca gtaccaggat taccagcgca cacagtatga
     1801 agtgtatgac taccccggac gtttcaaggg tgcccacggg cagaactttg cccgctggca
     1861 gatggatggc tggcgcaaca acgcagaagt ggcgcgcgga acaagccgtt cgccggagat
     1921 atggccggga cggcgaattg tgctgacggg gcatccgcag gcgaacctga accgggaatg
     1981 gcaggtggtg gcgagcgatc ttcacggcga acagccgcag gcggtaccgg gacgcagggg
     2041 ttcaggcacc acgctggata accactttgc ggtaatcccg gcagaccgga catggcggcc
     2101 acagccgttg ctgaaaccgc tggtggacgg cccgcagagc gccgtcgtga cgggaccggc
     2161 aggcgaggaa atcttctgtg atgaacatgg ccgcgtgcgg gtgaaattta actgggaccg
     2221 ctataacccg tcaaaccagg aaagttcatg ctggatccgt gtggcacagg cgtgggcagg
     2281 caccggcttt ggtaacctgg cgataccgcg tgtgggtcag gaggtcattg tggacttcct
     2341 caacggcgat ccggaccagc cgatcattat ggggcgtacc taccaccagg aaaaccgcac
     2401 ccccggcagc ctgccgggga cgaagacgca gatgacgatc cgctcgaaaa cctataaggg
     2461 cagcgggttt aatgaactga agtttgacga tgcgacaggg aaagaacagg tctacatcca
     2521 cgcgcagaag aacatgaaca ccgaggtgct gaataaccgc accactgatg tgataaacaa
     2581 ccatgccgaa aaaataggta acaaccaggc gatcaccgtt accaataact agatccagaa
     2641 cattggcgtt aatcagatac agacggttgg tgtcaaccag gtggaaacgg tgggcagtaa
     2701 ccagattatc aaagtgggat caaaccaggt tgaaaaggtg gggatcattc gtgcgctgac
     2761 ggtgggtgtg gcttaccaga cgacggtagg cggcattatg aatacctcgg tggcgttgtt
     2821 gcagtcctca caggtagggc tgcataaatc actgatggtg ggaatgggct acagcgtcaa
     2881 tgtggggaat aacgtcacct tctcggtggg caagacaatg aaggaaaaca ccggacaaac
     2941 agcagtttat tctgccggtg agcatcttga actctgctgt ggtaaggcaa ggctggtgct
     3001 gacgaaggac ggaagcatat ttctcaatgg tacgcacatt catctggaag gggagtcgga
     3061 tgtgaacggt gatgcgccag tgattaactg gaactgtggt gccacacaac ctgtaccgga
     3121 tgcgcctgtg ccgaaagatt taccccccgg aatgccggat atgcggcaat tttgagtcag
     3181 gtgcaaaaaa ttaaaacgga actggtgcag tgaccagata aaccatatta aaagcagaag
     3241 gaattgattc atgggcggaa aaccggcggc gcggcagggt gatatgaccc gcaagggact
     3301 ggatattgtg cagggttcag cgggggtgct gataggtgcg ccgacgggag tggcctgttc
     3361 ggtatgtccg ggagggatta cctatgctaa cccggtgaac ccgctgctgg gtgcgaaggt
     3421 gctgccgggc gagacggacc ttgcgctgcc cggcccgctg ccgtttattc tttcccgcgc
     3481 ctacagcagc taccggacca gaacacccgc gccggtgggg gtgtttggtc ccggctggaa
     3541 agcgcccttc gatatccgct tacagatacg cgatgaaggc ctgatactca acgacagcgg
     3601 cggcaggagc attcactttg agccgctgtt tcccggcgag ataagctaca gccgcagcga
     3661 gtcactctgg ctggcgcggg gcggagtagc ggcgcagcac agttcgcagc cgctaagcgc
     3721 gctctggcag gtgctgccgg aagatgttcg cctgagtccg catgtgtacc tggcgacaaa
     3781 cagccttcag gggccgtggt ggatactcag ctggccggag cgggtgccgg gggcggacga
     3841 ggtgctgccg ccggagccgc ccgcataccg ggtgctgacg ggcgtggtgg acggcttcgg
     3901 gcggacgctg gcctttcacc gggcggcgga gggtgatgtg gcgggcgcgg tgacgggggt
     3961 gacggacggc gcgggccgcc gttttcacct ggtgctgacc acacaggcac agcgggcgga
     4021 agtattccgt aaacagcgcg ccacgtcttt atcttcccct gccggtcccc gttctgcttc
     4081 ctcttctctg gttttccccg acacgctgcc cgccggtaca ggatacggca cggataacgg
     4141 tatccggctg gaggcggtat ggctgacaca cgacccggca tacccggatg aacagccgac
     4201 tgcgccgctg gcgcgctaca cgtacacggc cggtggtgaa ctgcgggcgg tgtatgaccg
     4261 cagcggaacg caggtgcgcg ggtttactta tgatgcggag cacgccgggc ggatggtggc
     4321 gcaccattat gcggggaggc cggagagctg ctaccggtat gatgataccg gtcgggtgac
     4381 ggagcaggtc aacccggagg ggctggacta ccgctttgag tacggagaga gccgggtaat
     4441 catcacggac agcctgaacc ggcgggaggt gctgtacacg gaaggcgagg gtggcctgaa
     4501 gcgggtggtg aagaaggaac atgcggacgg gagcattacc cgcagcgagt atgatgaggc
     4561 ggggaggctg aaggcacaga cggatgcggc ggggcggcgg acggagtaca gcctgcatat
     4621 ggcgtcgggt gcggtgacgg cggtgacggg gccggacggc aggacggtac ggtatggcta
     4681 taacagccag cggcaggtga cgtcggtgac gtacccggac gggcttcgca gcagccggga
     4741 gtatgatgag aagggaaggc tgacggcgga gacctcgcgc agcggtgaga caacgcggta
     4801 cagctatgat gacccggcga gtgagctgcc gacagggata caggacgcga cgggcagtac
     4861 aaaacagatg gcatggagcc gttacggtca actgctggcc ttcacggact gctcggggta
     4921 cacgacgcgg tatgagtacg accggtacgg tcaacaaatc gccgttcacc gggaggaagg
     4981 catcagcact tacagcagtt ataacccgcg tgggcaactg gtcagtcaga aggatgcgca
     5041 ggggcgtgaa atccgttatg agtacagcgc cgcaggcgac ctgacagcaa cgatatcacc
     5101 ggacggtaaa cgcagcacca tcgaatatga taaacgcggg cgtccggtga gtgtgacgga
     5161 aggcggtctt acgcgcagca tggggtatga cgctgccggg cgcatcaccg tgctgaccaa
     5221 cgagaacggc agccagtcca cgttccggta tgacccggtg gacaggctga ctgaacagcg
     5281 cggttttgac ggccggacgc aacgttacca ctatgacctg accggaaaac tcacgcagag
     5341 tgaagacgag gggcttatca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
     5401 gacggtgaac ggcgacccgg cagagcagtg gcagtatgat gagcacgggt ggttaaccac
     5461 cctcagccat acctgtgaag gccaccgcgt gtcggtccac tacggctatg acgataaagg
     5521 ccgcctgacg ggtgaacggc agacggtgga gaacccggag acaggggaga tgctgtggga
     5581 gcatgagacg gggcatgcgt acagcgaaca ggggctggcg acccgtcagg agccggacgg
     5641 tctgccgccg gtggagtggc tgacgtatgg cagcggttat cttgcgggga tgaagctggg
     5701 cggaacgccg ctggtcgagt acacgcggga ccggctgcac cgcgagacgg cccgtagctt
     5761 cggcggggcg ggcagtacag cggggtacga acaggccacg gcgtacaccc tcacagggca
     5821 gctacagagc aggcacctga accttccgca gcttgactgt gactatacgt ggaacgataa
     5881 cggacaactg gtgcgcatca gcggcccgca ggagtgccgg gagtaccgtt acagcggcac
     5941 gggaaggctg acgggcgtcc acactacggc agcgaacctg gatatcgata tcccgtatgc
     6001 gacggacccg gcaggaaacc ggctgccgga cccggaactg cacccggaca gcaccctcac
     6061 ggcgtggccg gataaccgca tcgcggaaga tgcgcactat gtctaccgct acgatgaata
     6121 cggcaggctg gcggagaaga cggaccgcat cccggaaggg gtgatacgga tgcacgacga
     6181 gcggacccac cactaccact acgacagcca gcaccgtctg gtgttctaca cgcggataca
     6241 gcacggtgag ccgcaggtgg agagccgcta cctctatgac ccgctgggcc gccggacggg
     6301 aaaacgggtg tggcggcggg agcgtgacct gacggggtgg atgtcgctgt cgcgtaaacc
     6361 ggaggagacc tggtacgggt gggacggtga caggctgacc actgtacaga cccaacagac
     6421 aagaatccag acggtatacc agccgggaag cttcacgccg ctcctgagaa tcgaaacaga
     6481 gaatggtgaa caggcgaagg cgcggcaccg tagcctggcg gaggtgttgc aggaggacac
     6541 gggtgtgacg ctaccggcgg agctggcggt gatgctgggg aggctggagc gggaactgcg
     6601 gcagggcagc gtgagtgaag aaagccagca gtggcttgcg cagtgcgggc tgacggcgga
     6661 acagatggcc gcgcaactgg aggcggaata catcccggag aggaaacttc atctttacca
     6721 ctgtgaccac cgtgggctgc cgctggcgct tatcagcccg gaaggggaaa cggcgtggca
     6781 gggggagtat gacgagtggg gaaacctgct gggcgaggaa agcgcgcagc accttcagca
     6841 gtcactccgt ctgccgggac agcagtatga tgaggagtcg gggctgtact acaaccgcaa
     6901 ccggtactat gatccgttgc aggggagata tatcacccag gacccgatag ggctgagagg
     6961 tgaatggaat ctgtataaat atccattaaa ccctgttcgg tttatagatt ctttgggttt
     7021 aaaattccat gttaacggtg atccaagtga ttttaatcag gctgttgagt atttaaaaca
     7081 agattcacaa atgaaagaaa ctatagattt tttatcaagc tctgaagaaa ctataaatat
     7141 agagtatatt gaaggaacca atgttagatt taattcgaat aatatggcga tatattggaa
     7201 tagtagagca tctttatttt gttcaactga attaaactcg aaatcacaat cgcctgcatt
     7261 aggtttggga catgagtttg ctcatgcgca gtattacctt cttgataagg aaaatttcat
     7321 ggctctttta agccgaacag ataagaaata tgagaataaa gaagaggcaa gggttattac
     7381 aataattgaa tcgcgtgcgg cgaaaacttt aggtgagtgt accagaggag ctcatagtgg
     7441 actgcctttt tatcgggtgg atggaccgtt gcagacgatg aaaataacgg gtacgccaga
     7501 atagtgaggt tttttatgat gaaatatttg atggttttat tgtctctatt ttctggttcg
     7561 gtactgggga tggggcgagt gaatgaattg tgtggaatag atagcgtgaa aacgatagag
     7621 ataattaatc ttccatcgta tgttaccacg ttagttccgc ttagcaagga aggtcttaat
     7681 gaaatataca gatataaagt ggtggtgaat gaaattagcg atctttatgc tggtaagata
     7741 attgatttat tgcaaatgaa gtatttcaga aaagaaaaat ataacaatat aagatggggg
     7801 gttagtatca tttcgaaagg taataataaa tgtgaaattt attttgatgc ttttggtgaa
     7861 tgtggaagtg tgaatggcat taacgtgtgt tttgaaaaaa atgaaatgat aggttggatt
     7921 aaaaaagaaa tccctttgct gtcgcaaaaa ataggtgggc tttaaatggt agggcaatat
     7981 tcaaagacag tataggtaac tatttgtatg gccatgtaag gtaaaagaaa aggaataaac
     8041 catgagcgga aaaccggcgg cgcggcaggg cgacatgacc cgcaggga
//
LOCUS       DAZGXZ010000196         7885 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000196 DAZGXZ010000000
VERSION     DAZGXZ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.196"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(39..737)
                     /locus_tag="ACHKJF_004833"
     CDS             complement(39..737)
                     /locus_tag="ACHKJF_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0952058.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIDGSGFEMNGKGSSARPSLTVSNLSGLVTGVAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMKASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(737..1066)
                     /locus_tag="ACHKJF_004834"
     CDS             complement(737..1066)
                     /locus_tag="ACHKJF_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952059.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWTPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1063..3567)
                     /locus_tag="ACHKJF_004835"
     CDS             complement(1063..3567)
                     /locus_tag="ACHKJF_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0952060.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTATVV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     AFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYSWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQASESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAVALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQGRSQRRGKTASFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAEREASQLKYTGEAQKAYERQLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYAVLKEAATKRQLSGQEKSLLAHEKETLEYKRQLADLGDKVEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLSDRQAQRESEAQRLRDVYGDNPQALAKATSALKNTWS
                     AEEQLRGSWMAGLKSGWGEWAESAMDSFSQVKSAATQTVDGIAQNIAAMLTGSEQNWR
                     SFTRSVLSMMTEILLKQAMVGIVGSIGSAIGFAGGGFTGTGGKYEPAGIVHRGEFVFT
                     KEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQ
                     AGPQLLKAVYDMARKGAQDELRLQLRDGGLLSGSGR"
     gene            complement(3548..3961)
                     /locus_tag="ACHKJF_004836"
     CDS             complement(3548..3961)
                     /locus_tag="ACHKJF_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0952061.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFFDPDMHPSDFSLLVPRHEEEQVEMPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(3988..4419)
                     /locus_tag="ACHKJF_004837"
     CDS             complement(3988..4419)
                     /locus_tag="ACHKJF_004837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0952062.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHADTDITEVAKNSALTDDDFSAGKSSTAS"
     gene            complement(4433..5185)
                     /locus_tag="ACHKJF_004838"
     CDS             complement(4433..5185)
                     /locus_tag="ACHKJF_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952063.1"
                     /translation="MATPNPLEPVKGAGTTLWVYIGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEEADWTATGQGQKSAGDTSFTLAWKPGEDGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWISSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     VTAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMAITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            complement(5193..5588)
                     /locus_tag="ACHKJF_004839"
     CDS             complement(5193..5588)
                     /locus_tag="ACHKJF_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0952064.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAITAIPALAGMITRM
                     VTQGYEYRRDDDMALWSSADLTYSITYGM"
     gene            complement(5585..6118)
                     /locus_tag="ACHKJF_004840"
     CDS             complement(5585..6118)
                     /locus_tag="ACHKJF_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952065.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVSRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     GELAYALKQQLRIAIKR"
     gene            complement(6133..6486)
                     /locus_tag="ACHKJF_004841"
     CDS             complement(6133..6486)
                     /locus_tag="ACHKJF_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0952066.1"
                     /translation="MRDFQNAFDAALAGVDSTIIEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(6479..6862)
                     /gene="gpFI"
                     /locus_tag="ACHKJF_004842"
     CDS             complement(6479..6862)
                     /gene="gpFI"
                     /locus_tag="ACHKJF_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0952067.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESWSEQNHTDDGEQLYNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            complement(6914..>7885)
                     /locus_tag="ACHKJF_004843"
     CDS             complement(6914..>7885)
                     /locus_tag="ACHKJF_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0952068.1"
                     /translation="RALFLELFFRRTVNFHTEEVMLDKITGKTPVAAYVSPVVEGKVL
                     RHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLRIITDNLKQEEH
                     AIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGKKWSEQDRDTFD
                     PTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNSQLETAVKDLGA
                     VVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIRCYGAIQDAQAL
                     SEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK"
BASE COUNT         1657 a         2588 c         1843 g         1797 t
ORIGIN      
        1 ccagaatcgc tgattcagtc tgtgtcataa accgggattt actgcgaaag tttattgatg
       61 gaaaggaaac cgccgaaatt agccaccatg ccgcgcagct cacacccgcg catgcattta
      121 ctgcatctgt ccttgcggat atccgtggtg gggttgtcga actcatccgc cactgccgga
      181 ccgttatacc cgcattcatc gccccggtaa tcccacatac aggtgttcgc cagcatgatg
      241 cgaccgggaa acagcgcccc gtccgtctcg gtcggtgtgg caagcacaaa cgaggccttc
      301 atggctgtca gctccgacat ctgctccacc acccagcggt cgctcagctc ctgctccggg
      361 tccgcttccg gattgcccgc cacaaaattc accgcatcca gaaaacgcgc atacacccgg
      421 cggcggacca ccgtggcacc caccaggctc tgcaaatcct ccgccactcc ggtgacaaga
      481 ccagacagat tggacaccgt cagcgacggt ctggcactgc tgcccttccc gttcatctca
      541 aagccgctgc cgtcaatcgg gtatgcctga tattcccgcc cctgccaggt gaccggctcc
      601 cctttttcat tcagctcatt gcagaaaaaa tagcgctcac cgccctgcac cgtcaggtca
      661 atttcccaga gcaccacccg cggtgactgc tctgatttaa ccgactcgtt aagactttct
      721 tcgtggatat cctgcatcag ttcaccacct gcttaaactc cgcgctgaac tcaacgcgca
      781 acatcccgac ccgcgcagac cacccggcac aggtcacctt tatctgtcgg tatgcataag
      841 gcggtgtcca cagaaacgcc ttccagccac cgtgctctgc caggaacgct tccagatgcc
      901 gggcctcctc ccgggtcacg gaaagcatca ccctgtacgt cttcaggtca gcattcagcc
      961 ccgccgccat acgctgcgaa tacccgtcac caaaacgcac ttcacgcacc gatggctgcg
     1021 agttcacctc catatccggc ttcactttcc agcgaaatgt tttcatcgcc cgctccctga
     1081 taacagaccg ccatcacgca actgcagccg gagctcatcc tgcgccccct tgcgggccat
     1141 gtcatacacc gctttcagca gctgcggccc cgcctgtccg ttgataccgt cattctgaat
     1201 caccacatgg ttgttctgat taaaattaat gccttccgcc cgccgcatct gcgccggact
     1261 tccggcaccg cccacataac caccttccgc atagccccgc atcaggcggt acaggttgcc
     1321 gacaccaatc cggctggttg cctccttagt gaagacaaac tccccgcgat ggactatccc
     1381 cgcaggctcg tatttgccgc cagtacctgt aaaaccacca ccggcaaacc caatggcact
     1441 accgatactc ccgacaatcc ccaccattgc ctgcttaagc agaatttctg tcatcatgga
     1501 cagcacggaa cgggtgaagc tgcgccagtt ctgttcactg ccggtcagca tcgccgcaat
     1561 attctgtgca ataccgtcaa cggtctgcgt ggccgcgctt ttaacctgcg aaaaactgtc
     1621 catggcactt tccgcccact ccccccagcc ggatttcagc ccggccatcc agcttccacg
     1681 aagctgctcc tccgcagacc aggtgttctt cagtgcagat gtggctttcg ccagcgcctg
     1741 cggattatcg ccgtacacat cacgaagccg ctgcgcttcc gactcccgct gcgcctgacg
     1801 gtcgctgagg ccgcgggctt ttgcgctgat ggcggcctgc ttcgcgctct gctgctgttc
     1861 aaaccgcgcc gcctgctgtg ccagctcatt cagccgtttc tggtgttcaa ccttgtcgcc
     1921 caggtcagcc agctggcgtt tgtactccag cgtctctttc tcatgggcca gcagggattt
     1981 ttcctgcccg gataactgcc gtttcgtggc ggcctctttc aggaccgcat actgattttc
     2041 cgctttccat aaatcacggc gctgctggct gattttttca ttcgcaccgc tgtgcttctc
     2101 cagcgtcctg agctcagttt cgagcgccag cagggctgca tgcgcctggt cttcctggcg
     2161 ctcaccggca gacaccttca caccggacgg ctttttctgc gtcgactcat aatccttttt
     2221 tgccgacgcc atcagcgtgt tgtaatccgc ctgcaggatt ttcccgtctt tcagtgcctt
     2281 gttcagttct tcctgccggg cggtatattt ttccagtggc gtcagctggc gctcatacgc
     2341 cttctgcgcc tctccggtat acttcagctg tgacgcctca cgctcagccc tgtcccttgc
     2401 cgccagttca ccggcttttt ccatatccga ctgcagcgtt gccgctgcca gacccagacg
     2461 ggcattttcc cggtcatccc atgcgccctg aaggttggcc cggaaagagg cggttttacc
     2521 gcggcgctgg ctccggccct ggtaccactg ccatttttta tccgcctcat caaatgcctt
     2581 ctgcgcactg gcgagcatat ccgcagaaga ttccggacga ccgatatcca gaatggcatc
     2641 ccacatcgat ttgaatgcct tccctgtttt atccgcccag gtctccagcg tccccatgtt
     2701 ttctttcagg cgacgggtct gctcatcaaa gccttttgtg gcgatatcgt tcgccgcctg
     2761 taaggccacg gcctcatcac cggaacgctg cagctgcgca acatacgcaa tctgctctgc
     2821 cgtcacgtta cggaactggc gcgccatcgc catcagtccc gacgtcgggt cggtggtcag
     2881 ttttccgaaa gcctcagcaa ccttgtccac ctccacaccg gatgcagatg caaaacgcgc
     2941 aacactctgg ttgatggcat caaactgttc accaccacgc acaccggcat tcaccagggc
     3001 tgccagtgac tcactcgcct ggttaaacgt cagccccgcg gcctgtccgg ctctggagag
     3061 cgtcagcatg cgatcggcag tcagtccgga ctgattaccg gaaagaacca gggttttatt
     3121 aaacgctgaa agcgtggaat ccccctggta ccaggaatac gccagcgcac ctgtcgccac
     3181 cgccagcgag gtgaccccga ccatcggcag ggtgatcgct ccggcaagcc ccctgaacat
     3241 ggggatcatc ccgccgaagg cgtccttcac ctgaccgccc tgttgcagca ggatgagcca
     3301 gggattctga ccaccggcaa gctgcgtggc gatatccgta aactgtgcgg gcagggttcg
     3361 catggccgct ttatactgcc cgacggaaat cccggctttt tgtgcagcca gcgcctggcg
     3421 gctcagcccc tgctcaacga cagtggcggt ttttctggcg tcagtatcca gtccggaaaa
     3481 atgacgcctt acccggctca tctgctcatc gaaacggacc gcatccagac tcaggtcaat
     3541 aacaagatca ccaaccggct gggacatatc tcactcctcc cggaatcccc gctgaagcca
     3601 tcattaatgc ggcatcatcc accatgacat ccgccacatc cgcagacgat aaaatatcgc
     3661 gccctccgtc cccaccgaac cggacgcctc cggcaagtcc tgccgctttc tgcatcagca
     3721 ttttgtcctc atccggcatc tccacctgct cttcctcatg ccgggggaca agcagactga
     3781 aatcagaggg atgcatatcc ggatcgaaaa aaaacaggct gagtacagcg tacgtcagcc
     3841 cggaaaaatg catatccagc tgggtatcgt gaaaataatg cgtgcggtaa aaacgtcgcc
     3901 agtcggcata ttcggtggat gtcatcccgg caagcatggc gcgccagtca ggcctcccca
     3961 tctcacgcgc cagtctgagg gcaaaattca gctcgccgtc gaagactttc ccgcagaaaa
     4021 atcatcatca gtcagcgcgc tatttttcgc cacttcggta atatcagtat ccgcatgaac
     4081 aggcccgctc atcccggaca aacgcaacac cacatcttcc gcccgggcaa tggcatcagc
     4141 aggccaggtg gtcaggactt cctgctcaat ctgcatcacg gcctcattca ttgacggtga
     4201 ccccgttttc tgcggatggt tatgccacag agacatcgcc accagaaacg caccggttct
     4261 gacgagatct tccacgctta cctgcaggtt gccgctggat tctgcctgtt ctgcccgccg
     4321 tttcaggagc gcaagatgct caatacgctg cagcgcagac agttcagaaa gcgtgacgga
     4381 cacaccgtta tattcaaatt gttctgtttt cagaaacatg tattacctcc gtttaccctg
     4441 cagcgcccgc ttcagtaacg gtgacttcag ctaccgtggc aaactgacca ttaccggaaa
     4501 tcacggggat actcactttt ccagccttaa cccccgtcac agtgatcgcc atatctttca
     4561 cagcaatggt tcccgttgac ggatcggcgg aaaccgctct gaacgtcttg tcggttgcac
     4621 tttccggctc aaaagaaaca gtcagggtgg ttgtttttcc tttttccacg gtaccggatg
     4681 tcggcgtcac cttaattgca gtgaccggcg taatttcgct gcgttcttcc gccacggaag
     4741 gtttgcccac gttggtaact ttcactgttc gggtgatcac ttctttcgcc gtcactgcct
     4801 taccgatact gctgatccag ccacgaaaca catccaccgt gccattcgga aaacggattt
     4861 tataggcccg gacatcgccg ctttcaaacc agcctataag ccctttctga ccgtcctctc
     4921 ccggtttcca ggccagcgta aaactggtat cacctgcaga tttctgtccc tgcccggtcg
     4981 cggtccagtc cgcttcttca tcatccaggt agttatcatc gtaggattct gccgtcatct
     5041 cgcccggcgt cagatccttc accttagcca gtcgctgcca gtcatcgtct gacaacgggt
     5101 ttgcataagc atcacccgtg ccgatgtaaa cccacagtgt ggttccggca ccttttaccg
     5161 gctccagggg atttggtgtt gccatatcgt cctcacatcc cgtatgtaat tgaataagtc
     5221 agatctgcag aactccacaa tgccatatcg tcatcacgac gatactcata gccctgcgta
     5281 accatccggg taatcatgcc tgccagtgca gggatcgcag ttatcgccgg gtaaatccgg
     5341 ctttccatcc actgatcaag ctctgaatcc ggtacctgtg ccggtaaaaa cacctcaata
     5401 tgcagtgtgg cccgccaggt atctgcatcc agctcttcac cggtatactc tgcatccgtc
     5461 agataaaccg caatcgcagg aaaatcctct tcgtcaaaaa caacggggcg accatcaaac
     5521 agcgtcgccc cgtgttcatg ctgctcgagt gcatccagca ctgcagcacg gatatcagta
     5581 tgtttcatcg ttttatcgca atcctcagtt gttgtttcag cgcgtatgcc agttctccgg
     5641 gcaggcgttc acgccggata cggtcaacgt tttcatcaaa cgcctgtttc agtggggccg
     5701 ccatcgggat tttcaccaca tcaatggggt aacggttttt cccggcaaca cgctgcatga
     5761 catgccagcg accatttttt aatcgctgaa taaatgcccg ctgataccga tgctgaccag
     5821 ctttaagtat gctgttcgga cgacggccca gcatcctgat ccccagctta atcacaggga
     5881 gatcaccgcg gttaacgata attctggcat tcggatttct gaccgtcgcc cgtttcagtc
     5941 tggaccgttc ctttaccagt ttccggctca ccctggtctc ccgggcaacc tgtgatgaag
     6001 actgattaat cgccgttgtg gcaacgcggt taatagccat tgctgaagcc gccggaatgg
     6061 cgtttttacg aacccggctc agattttcaa tcgcctgatc aagccctttt atcgccataa
     6121 ttcaccctgc gtttatcgtc gccggttaac tgcggggggt tgcccacggt tgagccagag
     6181 atagcaactg cccccgtcat ccggagaaac acgatccacc cagaatgtct caccattaat
     6241 ggtcagcgtg tcaccacgcc gcacagcacg aaccgtatcc gtccgcacaa ataatgacgg
     6301 gcagcttcct tcaatacgga ccccgctacc ggcaaacccc agcgactccg gatcgtcaaa
     6361 aaccccctga acttcgctgc cacactgtgc ccccgaggtg aactgcgcac agagtcccat
     6421 cacttcaata atcgtgctgt ccaccccggc aagggcagca tcaaaggcat tctgaaaatc
     6481 acgcatgctc agccgttccg tgctgtatca tggctgtcgc cagtggtgac ggcaccagaa
     6541 cacgcatgcc ccggtacgtc agttcaacgg gacggcctgt ctccgggcaa taccccatca
     6601 catgcaggca tttccgcaca cggaccgctt taacatcatc cgtagcatca gtgttgtaca
     6661 actgctcacc atcgtctgtg tgattttgct ctgaccagct ctcatcacca ggcataatgc
     6721 cctcccggga agcagcaagc tcctcttccc actcagacac acgttgcgca atatccgcag
     6781 cactccccga catatccgcc tcgcgcccca gcaggccagc cagttgacga agacgattca
     6841 gattttcttc ttttgttgcc atatcagccc cctgtgaaaa aagacacggg ggcatttcgc
     6901 ccccgctcac ggattatttc acctgcacca ccacaaactc atccggatcc ggcagcacca
     6961 tcagcggtgc ggactgcgtc atggtaaatt cacgggccgg atcgcccacg gtcagccagt
     7021 gtttcgggta acgggaagaa gccaccacac cttcggacaa cgcctgcgca tcctgaatgg
     7081 caccgtaaca acggatccca tctgcagccg tatttcccag caccagcgtg ccctccggca
     7141 gataacgttt ttcggtaccg tcctctgcca cataagacgt tttcgccacc acaatggcca
     7201 gatcgccgta ataccctttg aaagacacca ccgcccccag gtctttcact gccgtttcga
     7261 gttgtgaatt tgagccgcga cgggtatcca gtttttcgcg gaaaagctta aagccattca
     7321 gcagacgcca gacagtaccg tccataatgg caatattcac aagaccgctg gcctgatcgc
     7381 agtaaaggtc aatatcatgc gtcggatcaa acgtatcacg gtcctgctca gaccattttt
     7441 taccgtcggc ctgctcaatg ttatttcctt cagagcgtcc aaaatccacc tcgacagtat
     7501 caaactgatc cccttccatg gtgtatttgc catacagcac ggcattcacc gcctgcattt
     7561 cttccacctg gacaatggcg tgctcttcct gtttgaggtt atcagtgatg atacgcagac
     7621 gacggtaggc cgggtcgttc agctgagccg gatcttcacc gggaagacgc tcaaccgcct
     7681 gctggtaatt aaattcgtgt ttcggcttga cgtagcccgg acgcaacacg cgggtttcac
     7741 caccgcgatg acgcagcact tttccttcaa cgaccgggga gacataggcc gccaccggcg
     7801 tttttccggt aattttgtcc agcatcacct cttcggtgtg gaaattcacc gtacggcgga
     7861 aaaacagttc cagaaacagc gctcg
//
LOCUS       DAZGXZ010000197         7868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000197 DAZGXZ010000000
VERSION     DAZGXZ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.197"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(185..1378)
                     /gene="tyrB"
                     /locus_tag="ACHKJF_004844"
     CDS             complement(185..1378)
                     /gene="tyrB"
                     /locus_tag="ACHKJF_004844"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP0952069.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEVLKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1631..2710)
                     /gene="alr"
                     /locus_tag="ACHKJF_004845"
     CDS             complement(1631..2710)
                     /gene="alr"
                     /locus_tag="ACHKJF_004845"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP0952070.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2763..4178)
                     /gene="dnaB"
                     /locus_tag="ACHKJF_004846"
     CDS             complement(2763..4178)
                     /gene="dnaB"
                     /locus_tag="ACHKJF_004846"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0952071.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4261..5244
                     /locus_tag="ACHKJF_004847"
     CDS             4261..5244
                     /locus_tag="ACHKJF_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP0952072.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5410..5652)
                     /gene="pspG"
                     /locus_tag="ACHKJF_004848"
     CDS             complement(5410..5652)
                     /gene="pspG"
                     /locus_tag="ACHKJF_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP0952073.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(5786..6823)
                     /gene="dusA"
                     /locus_tag="ACHKJF_004849"
     CDS             complement(5786..6823)
                     /gene="dusA"
                     /locus_tag="ACHKJF_004849"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP0952074.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7186..>7868)
                     /locus_tag="ACHKJF_004850"
     CDS             complement(7186..>7868)
                     /locus_tag="ACHKJF_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP0952075.1"
                     /translation="CTDEDLNDRNFIASVVDRAIFHFAINSICNPGDNKDATPIEQCT
                     FDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFAS
                     LLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKRLADGGEMNF
                     DGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKILTDTSKLIYD
                     LFGVKSEK"
BASE COUNT         2041 a         2045 c         1873 g         1909 t
ORIGIN      
        1 acccaaaaaa acaggataag gaattccaga taattaggat aagcttaaca ataaaagaac
       61 taataacgtt tattatctct cacgtagaac gatggcatca aaaaaatcat ctataaagaa
      121 agagtgaaag cgacgacagt ttaatttcac tgcaggctgg gtagctccag cctgctttcc
      181 tgcattacat caccgcagca aacgcctttg ccacacgttg tacatttgcc gtatttaacc
      241 cggcgacaca catgcgaccg ctggcgatga gatagacacc aaattcttca cgtagtcggt
      301 caacctgagc ggcacttaaa ccggtataac tgaacatgcc gcgctgatta agcagataat
      361 cgaaattgcg ttctggcatc tctgtgctta ataccttcac caattcctga cgcattgcca
      421 gaatgcgagt acgcatctct tctacttccg ccagccagct ggctttcaat acctcgtcat
      481 tcagcactgc agccaccacc tgcgcaccaa aattcggcgg gctggagtag ttgcggcgaa
      541 ctgttgcttt caattgcccc agtacgcggc cagcggcttc ggcatcttca cacagaacag
      601 aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt cgagaacgaa ttgctcacca
      661 gagcgggtaa tccagcgctg gcaatggcgc gaatagcgta ggcatcctct tccataccgg
      721 caccaaatcc ttgataggca atatcgagga atggaataag ctcgcgggct ttgagaattt
      781 caatcaccgc atcccactga tcattagtga gatcggcacc cgttgggttg tggcaacatg
      841 gatgcagcaa cacaatactg cgggcaggta atgttttcag cgtcgccaac aggtcattaa
      901 agcgcacgcc gttagtcgct tcgtcatacc aggggtaagt actcacttcg aatccagccc
      961 cggcgaatat tgctacgtgg ttttcccagg taggatcgct gacccagacg cctgattccg
     1021 ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc ccctgagccg ccaagggttt
     1081 gaatggttgc tacgcgctgt tgttgcagta ctggatggtc cgcaccaaac agcagcggcg
     1141 caatggcatg gcgatagctg ttaagccctt ccatcggtaa ataaagcgaa gcgccatgag
     1201 gctgcgcatt caggcgcgct tccgcctccg ccacggcttg cagttgtgga ataattccgt
     1261 cttcgttgta gtacagaccg atacttaaat tcactttgtc gctgcgaggg tcttctttaa
     1321 aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc gtcaactttt tgaaacacgc
     1381 gatggttctc caggtttacg ggcaggtggt taaaacacaa taaaccggaa gaaggcgaag
     1441 atcgagtgga tgttcaggag cgaacggcaa ttagcaacag agtgagactc atgacaaacg
     1501 tacatccgcc agaggcacga cctttataag agcatgaaag aacatcaact tattgaattt
     1561 ttaggatttt attggccgga taaggcattc acgccgcatc cggcacagac aatcaaatat
     1621 tacagaacga ttaatccacg tatttcatcg cgacccttga agtcaggcgc gtaataagtt
     1681 cgtaagcgct tactttcgtc atttcagcga tacgttctac gggcaaacct tcgccccata
     1741 aaatgaccgg atccccggct ttgtcctgcg cttgtggacc taagtctacg cagatcatat
     1801 ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt caccagcact ggcgtaccgg
     1861 acggcgcggc gcgcggataa ccatcgccat agcccatcgc gactacgcca agacgggtat
     1921 cacgttcgct tacccaggtt ccaccataac caacaggttc tccggcttta tgctcacgca
     1981 cggcaatcag gctggaggtt agtgacatca ctggctgaca gccaaaatcg gcaccggtgg
     2041 agcgatcttc cagcggcgag acgccataaa gaatgatgcc cgggcgcacc cagtcaaaat
     2101 gcgactgtgg ccacagcaga atgccacccg atgcggcaat ggaacgttga ccaggtttgc
     2161 cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt tgcgccacat tttggttcat
     2221 ccgcgcgcgc aaaatggctg acgatattca ccggctgacg aacgtttttg cactgggtca
     2281 ggcgatgata aaacgcctca gcctgttccg gccttacgcc cagacggtgc ataccggtat
     2341 cgagtttcat ccagacggta accggctcgt ccaggctagc ctcttccagc gcagccagct
     2401 gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga aatcgtcggc agatctctgg
     2461 catcaaaaaa gccttcgagt aacagtacag gtttggtgat tccccccgca cgcagtcgca
     2521 gagcttcttc gagacgggct acgccaaagg cgtcagcatc ggggagcgtt cgcgcggtct
     2581 caagaagacc gtgaccataa gcgttcgctt tcaccaccgc aaccatttta ctggcagggg
     2641 ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg gcggttaatc acaacagttg
     2701 ccgcttgcat ttgtgttcct tgataagtgt ttgctttaat tacctaattc ataaaataat
     2761 tattattcgt cgtcgtactg cggccccgca tagttgtcga agcgcgacca ttgaccgtta
     2821 aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt taccgataat aatttccgcg
     2881 atgcctttta aatcactgtt ttcgtgatac acctcatcac gatagataaa catgatcaag
     2941 tccgcatcct gctcgataga gccagattca cgcaggtcgg agttgaccgg gcgtttgtcg
     3001 gcacgttgtt ccagagaacg gttcaactgg gacagcgcca ccaccggcac gttcagttct
     3061 ttcgccagtg ctttcagcga gcgggagatt tctgcaattt ccagcgtacg gttatcggaa
     3121 agcgccggta cgcgcatcag ttgcaggtag tcgatcatga taagcccgat gccgccgtgt
     3181 tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg gcgtcaggcc ggaggagtca
     3241 tcgatataga tattgcgttt ttcgagcaaa atacccatgg tgccggaaat gcgcgcccag
     3301 tcttcatcat cgagctgccc ggtacggatt ttagtctggt caacgcgcga cagcgacgcc
     3361 agagaacgca tcataatctg ttctgaaggc atctccagcg agaagataag taccggttta
     3421 tcctgcaaca tcgccgcgtt ttcgacgagg ttcatcgcaa atgttgtttt acccatcgac
     3481 ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc cagcggtttt tttgttgaga
     3541 tcgtcataac cggtgtttac tccggtaacg ccatcgtgtg gctgctgaaa caactgctca
     3601 atacgcgcca cggttgcgtc gagcacatcg gcgatgttct tcggcccttc gtctttgttt
     3661 gcacgacttt cagcaatttt aaagacgcgg gattcagcaa ggtccagcag atcttcgctg
     3721 gtgcgcccct gcggatcaaa accggcttca gcaatctcat tcgcaaccga aatcatctca
     3781 cggacaacgg cacgttcacg cacgatgtcc gcataggcac tgatgttcgc cgcacttggc
     3841 gtattttttg acagctctgc cagataagca aaaccaccga cgctatcgag ttgcccctgg
     3901 cgttccagcg attccgcaag ggtaatcaga tcgataggac taccgctttc ctgcaaacgc
     3961 gccatttcag taaagatatg gcggtgtggg cgggtgtaaa aatcgtctgc caccacacgc
     4021 tcggctacat cgtcccagcg ttcgttatcc agcattaaac cgcccaacac cgactgctcc
     4081 gcttcgatcg agtgcggagg cactttcagc ccggcaactt gtggatcgcg ttcgcggggt
     4141 tcagcctgct gtttgttgaa gggtttattt cctgccatag tgaatggagt taccgagata
     4201 aagaatgggt cgaaacttta ccatatgaag cagaccctga cgatacgttc tggaggacac
     4261 atggcaacac gaattgaatt tcacaagcac ggtggcccgg aagtacttca agccgtagag
     4321 ttcactcctg ccgatccggc agagaatgaa atccaggtcg aaaataaagc catcggcatc
     4381 aattttatcg acacgtatat ccgcagcggc ctttacccgc cgccatcact acccagcgga
     4441 ttaggtaccg aagcggcagg catcgtgagt aaagtcggca gtggtgtaaa gcatattaag
     4501 gcaggcgatc gtgttgtcta tgcgcagtcg gcgttaggcg cttacagctc tgtgcataac
     4561 attaatgcgg ataaagcggc gattctgcct gcggcaattt cttttgaaca agctgcagca
     4621 tctttcctga aaggcttaac ggtttattat ctgctgcgca aaacctatga aattaaaccc
     4681 gatgaacagt tcctgttcca cgcagcggct ggcggcgttg gcttaattgc ctgccagtgg
     4741 gcaaaagccc tgggcgcgaa acttatcggc accgtaggaa ccgcgcaaaa agcgcagagc
     4801 gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg aagagaatct ggtcgagcga
     4861 ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt acgattccgt gggcagagac
     4921 acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct taatggtcag ttttggcaac
     4981 tcatcaggtg cggttaccgg tgtgaactta ggcattctca atcaaaaagg ctcgttgtat
     5041 gtgacacgcc cttccctgca aggctatatc accacgcggg aggaattaac cgaggccagt
     5101 aatgaactgt tctctttgat tgccagcggt gtgattaagg tcgatgtcgc cgagcagcag
     5161 aaatatccgc tgaaggatgc gcagcgtgcg catgagattc tggaaagccg ggcgacgcaa
     5221 ggttccagcc tgctgattcc ataaaagaaa tagggcttcc acctgggaag ccctttcttt
     5281 ttatagttcg gctgtatgta gggtacagca cgatgaatct gttagaggcg caatagtgac
     5341 agatttgatt atcaattcct attttgttct aaggataaaa ccttaggttg tgatcatccg
     5401 cacaatccct tagtaacgcc agcggtcata acgctgatat ttcggcattt ttggtgcttt
     5461 aatcgcctta ataacccaca ccaccgcaat cgccagtagt aaccacggca gcaacttaat
     5521 catcaatgcc agcataccgc cgaggaacat aatggccgtc gccacaacca gcgcggcgat
     5581 aatgcccagc aacgaaacgc cggtgaccat cagcatgaca aaaaagccaa tcacaaaaag
     5641 tagttccagc atgatgctct cccaaatatg aaatctcttg ctggcattac aagaatcatg
     5701 ccaaaaataa tctattgatt taacagcaaa acgccccgcg acggtgcgca gggcgtggtg
     5761 aatttgatta ctttttggtg aaaagttaac gcttatccgc caccagtttg agcgcgtgtt
     5821 ccagcacgtt aatgtcagca cccgctttat gggcattttc acttaaataa cgccgccact
     5881 gccgcgcgcc aggaataccc tggaacaaac ccagcatatg ccgggtaata tggccgagat
     5941 acgtcccctg gctgagttca cgctcaatgt acggatacat ggcgcgcact accgccaccg
     6001 gatcggcatc ggtatccgag gaaccaaaga tctcccggtc taccgccgcc agaatacccg
     6061 gattctgata cgcctcgcgc ccgaccatca cgccatccat atgttgcagg tgtgctttgg
     6121 cctcttccag cgacttgata ccaccgttaa tcgacattgt cagatgcgga aagtcacgct
     6181 tcagttgata cacacgcgga taatcgagcg gcgggatttc acggttttct ttcgggctta
     6241 accccgaaag ccaggcttta cgtgcgtgga tgataaacat ctcacactcg cctttgccgg
     6301 aaacggtgtt gatgaaatcg cagagaaatt catagctgtc ctggtcatcg atgccaatac
     6361 gcgttttcac cgtcactgga atcgacacca catcgcgcat cgctttcacg cagtcggcaa
     6421 ccagctgcgc attacccatc agacacgcac caaacatgcc gttctgcacc cggtcagacg
     6481 ggcagccgac attcaggttg atctcatcat atccacgcgc ttctgccagc ttcgcacact
     6541 gtgccagcgc cgccggatcg ctaccgccga gttgcaacgc taccggatgt tcttcttcac
     6601 tgtacgccag ataatcacct ttaccgtgaa taatcgcccc tgtggtcacc atttcggtat
     6661 aaagcaacgt attgcgggaa agcagacgca agaaatagcg gcaatgtctg tccgtccagt
     6721 cgagcattgg tgcaacgcta aaccgaccac tccagtgaac gtcagttttt tcaggcattg
     6781 cgctggtttg gttgattttt tgcatttcag aattaccgtg cattttcaaa tgtagagatt
     6841 attttatcaa tatatcatgg ggttatgtta ttcagcttca ctgttcagga ggctcaatag
     6901 cggggtacta taccataaca acaggaagcg cctgtctcat tgcaaaagaa aattgagatc
     6961 ctctcaaggc atgaagctct cacgaagtga tggaaataat cttattagcc gttaactttg
     7021 ttaaggccaa tgataaacaa tccaggttcg acgataaata aaaaatcaca cattaaactc
     7081 tggtgatata tctccctgct aatagcattg atagagaaaa aagaacccaa taaatattgg
     7141 gtccttttat ataatgcctt ccatactatc gaagaacttc acatattatt tctccgattt
     7201 aaccccgaac aaatcataaa ttaatttaga agtgtctgta agtattttaa tctcttcctt
     7261 tgaggttggg tcaaacgact tcgcgaagct aattaattgt ggtgcagcat ctcgcatttt
     7321 gcttaaaata tcaggatcga gcgttccttc attcaccaga tgtgacatct tatccagata
     7381 gccatcaaaa ttcatttctc ccccatcagc cagacgcttc atctctccca ggtacttctt
     7441 catatcacgt tgagataatt tttcaaactg agctttcagg tagctctcat tattttcatt
     7501 aatatacact gtttctgata aatctgcgta ggcactagaa tatgaaagtg aaattaacag
     7561 cgaagcaaat aattttaagc tgttgtcatc atgttcctgg caggcattaa caaacgttaa
     7621 aaaaccagaa cctattgctt gaaaatgtat attcgctaaa ggttcattat ccttagattc
     7681 ctcataaaat agctgaaccg tggagggaag gccattctta gtttctacat caaaagtaca
     7741 ttgttcaatg ggagtcgcat ctttattatc cccaggatta catatactat taatcgcgaa
     7801 atgaaaaata gcccggtcaa caactgatgc aataaagtta cgatcgttta aatcctcatc
     7861 agtgcaat
//
LOCUS       DAZGXZ010000198         7765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000198 DAZGXZ010000000
VERSION     DAZGXZ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.198"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(187..450)
                     /gene="cedA"
                     /locus_tag="ACHKJF_004851"
     CDS             complement(187..450)
                     /gene="cedA"
                     /locus_tag="ACHKJF_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP0952076.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWATQIRQEGEVTE"
     gene            633..2894
                     /gene="katE"
                     /locus_tag="ACHKJF_004852"
     CDS             633..2894
                     /gene="katE"
                     /locus_tag="ACHKJF_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP0952077.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2941..3699)
                     /gene="chbG"
                     /locus_tag="ACHKJF_004853"
     CDS             complement(2941..3699)
                     /gene="chbG"
                     /locus_tag="ACHKJF_004853"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP0952078.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVASFAAEEGIALRIDRQPLSNTG
                     DLPANLRSSHGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3712..5064)
                     /gene="celF"
                     /locus_tag="ACHKJF_004854"
                     /gene_synonym="chbF"
     CDS             complement(3712..5064)
                     /gene="celF"
                     /locus_tag="ACHKJF_004854"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0952079.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5169..6011)
                     /gene="chbR"
                     /locus_tag="ACHKJF_004855"
     CDS             complement(5169..6011)
                     /gene="chbR"
                     /locus_tag="ACHKJF_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP0952080.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6019..6369)
                     /gene="chbA"
                     /locus_tag="ACHKJF_004856"
     CDS             complement(6019..6369)
                     /gene="chbA"
                     /locus_tag="ACHKJF_004856"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP0952081.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6420..>7765)
                     /gene="chbC"
                     /locus_tag="ACHKJF_004857"
     CDS             complement(6420..>7765)
                     /gene="chbC"
                     /locus_tag="ACHKJF_004857"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP0952082.1"
                     /translation="ASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAMFVLIN
                     NVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFIGMALA
                     EERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVAEMFTF
                     IVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQIIMDTIS
                     TPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQYGSVE
                     AALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIF
                     QINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLG
                     AFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
BASE COUNT         2091 a         2023 c         1734 g         1917 t
ORIGIN      
        1 ctcatcatga ttagcaaaac ttaaccattt taaaataaat aaacaattaa agaaaaaaga
       61 tcacttattt atagcaatag ctcgtcaaag gcagcttttt gttacaggtg gtttgaatga
      121 atgtagcaac gaaatgcaga atttcaggtc atgtaactcc cggcaaaacc gggaggtatg
      181 taatccttac tcagtcactt ccccttcctg gcggatctga gttgcccaac gttgggcaga
      241 ttcaggcaca gtaaacgccg gtgagcgcag aaatgactct cccatcagta caaacgcaac
      301 atatttgcca cgcagcatcc agacatcacg aaacgaatcc atctttatcg catgttctgg
      361 cggcgcgggt tccgtgcgtg ggacatagct aataatctgg cggttttgct ggcggagcgg
      421 tttcttcatt actggcttca ctaaacgcat attaaaaatt agaaaaactg tagtttagcc
      481 gatttagccc ctgtacgtcc cgctttgcgt gtatttcata acaccgtttc cagaatagtc
      541 tccgaagcgg gatctggctg gtggtctata gttagagagt tttttgacca aaacagcggc
      601 cctttcagta ataaattaag gagacgagtt caatgtcgca acataacgaa aagaacccac
      661 atcagcacca gtcaccacta cacgattcca gcgaagcgaa accggggatg gactcactgg
      721 cacctgagga cggatctcat cgtccagcgg ctgaaccaac accgccaggt gcacaaccta
      781 ccgcccctgg gagcctgaaa gcccctgata cacgtaacga aaaacttaat tctctggaag
      841 acgtacgcaa aggcagtgaa aattatgcgc tgaccactaa tcagggcgtg cgcatcgccg
      901 atgatcaaaa ctcactgcgt gccggtaacc gtggtccaac gctgctggaa gattttattc
      961 tgcgcgagaa aatcacccac tttgaccatg agcgcatccc ggaacgtatt gttcatgcac
     1021 gcggatcagc agctcacggt tatttccagc catataaaag cttaagcgat atcaccaaag
     1081 cggatttcct ctcagatccg aacaaaatca ccccagtatt tgttaggttc tctaccgttc
     1141 agggtggtgc tggctctgcc gataccgtgc gtgatatccg tggctttgcc accaaattct
     1201 ataccgaaga gggtattttt gaccttgttg gcaataacac gccaatcttc tttatccagg
     1261 atgcgcataa attccccgat tttgttcatg cggtaaaacc agaaccacac tgggcaattc
     1321 cacaagggca aagcgcccac gatactttct gggattatgt ttctctgcaa cctgaaactc
     1381 tgcacaacgt gatgtgggcg atgtcggatc gcggcatccc ccgcagttac cgcaccatgg
     1441 aaggcttcgg tattcacacc ttccgcctga ttaatgccga agggaaggca acgtttgtac
     1501 gtttccactg gaaaccactg gcaggtaaag cctcactcgt ttgggatgaa gcacaaaaac
     1561 taaccggacg tgacccggac ttccaccgcc gcgagttgtg ggaagccatt gaagcaggcg
     1621 attttccgga atacgaactg ggcttccagt tgattcctga agaagatgaa ttcaagttcg
     1681 acttcgatct tctcgatcca accaaactga tcccggaaga actggtgccc gttcagcgtg
     1741 tcggcaaaat ggtgctcaat cgcaacccgg ataacttctt tgctgaaaac gaacaggcgg
     1801 ctttccatcc tgggcatatc gtgccgggac tggacttcac caacgatccg ctgttgcagg
     1861 gacgtttgtt ctcctatacc gatacacaaa tcagtcgtct tggtgggccg aatttccatg
     1921 agattccgat taaccgtccg acctgtcctt accacaactt ccagcgtgat ggcatgcatc
     1981 gtatggggat cgacactaac ccggcgaatt acgaaccgaa ctcgatcaac gataactggc
     2041 cgcgcgaaac accgccggga ccgaaacgcg gcggttttga atcatatcag gaacgcgtgg
     2101 aaggaaataa agttcgtgag cgcagtccat cgtttggcga atattattcc catccgcgtc
     2161 tgttctggct aagtcagacg ccattcgagc agcgtcatat tgtcgatggt ttcagttttg
     2221 agttaagcaa agtcgttcgt ccgtatattc gtgagcgcgt tgttgaccag ctggcgcata
     2281 ttgatctcac tctggcccag gcggtggcga aaaatctcgg tatcgaactg actgacgacc
     2341 agctgaatat caccccacct ccagacgtca acggtctgaa aaaggatcca tccttaagtt
     2401 tgtacgccat tcctgacggt gatgtgaaag gtcgcgtggt agcgatttta cttaatgatg
     2461 aagtgagatc ggcagacctt ctggccattc tcaaggccct gaaggccaaa ggcgttcatg
     2521 ccaaactgct ctactcccga atgggtgaag tgactgcgga tgacggtacg gtgttgccta
     2581 tagccgctac ctttgccggt gcgccttcgc tgacggtcga tgcggtcatt gtcccttgcg
     2641 gcaatatcgc ggatatcgct gacaacggcg atgccaacta ctacctgatg gaagcctaca
     2701 aacaccttaa accgattgcg ctggcgggag acgcacgcaa gtttaaagca actatcaagg
     2761 tcgctgacca gggtgaagaa gggattgtgg aagctgacag cgccgacggt agttttatgg
     2821 atgaactgtt aacgctgatg gcagcacacc gcgtgtggtc acgcattcct aagattgaca
     2881 aaattccggc gtaaatatgg ataaggtcac gtcccctggg gagtgacctt accaccttac
     2941 ttacacatca agataactac ccaaccgata accacgctca gcaattgcat atttcaacga
     3001 cgctgatgtc agcacttcca gctccgttaa ccgtgggaaa cagtaagcac tcttgcgaat
     3061 caaattatcg acaaatgccg gatggcacat gacttccagc gagcgttccc cgcgatggct
     3121 ggaatcatca agcacttgca ggaacagtgc ctcgctaatc tcttcaccat aaaacgcgct
     3181 actgaatccg tgtgaactgc gcagattagc cggtaaatca ccagtgttag atagcggctg
     3241 acggtcgatg cgcaacgcaa tcccctcttc agccgcaaag ctggcaacaa tcgggaaaat
     3301 ctgcgggaac atatgcacat gatgatggct atcaagatgc gtaggtttgc gtccaaatag
     3361 ctcaatgaaa cgcaaatact gactggcaag ctcctgagta atttcttcca gcggtaaagc
     3421 atcttcttct gccaactgcc agatccattt tcccagcaca ccatcgcggg ttaaccccgg
     3481 catagctgtc agtggcttgc ccatagtaag gacaaagtgc atccctatgg ccagactcgg
     3541 ttcatcacga ctcaactgca ccgcatggtc aatagcctgc ccattcacca gcgccgtcgt
     3601 cgatgtgaca atcccattgc gacaggcctc gataatgccg tagttctgac ctttgcttaa
     3661 gccaaaatca tcggcattaa caatcagtaa gcgttccata atcagcctcg gttaatgtgc
     3721 ttttttaagc tctgcgatgc agtcggcaaa atttggcagc catttctcgt gcgccagaat
     3781 catctcgcgt gccagcagct cagcatcgcg atcggaatgc accaacggac taaggtttag
     3841 cgccagtaac acatcgttaa attctccgct aagtgcggcg ttgctggcag caatctcgaa
     3901 gcctttaatg gtgtgaatca gccccatcac tttatcatcg aaatgcgtaa tgcgcggatg
     3961 tggcgtcgcg ccatcgcgcc ccagcttaca ggtcatttct accgcccagt ctgccggaat
     4021 attatcaata tgcccatgat gcgggatatt aacgtaatgt tcagcttgct tgtcgttgta
     4081 gatagcgttg atcacttcgc acgctgcatc agagtaataa gccccaccgc gctgttccag
     4141 ttctttcggc ttaactttca actccggatt tttatacagc tcaaaaagtt gtttctcgac
     4201 tttctgtact acctgtgctc gtgcgccgcc tttgtagtat tcgcccattt caatagccag
     4261 catctctttc tgcttgaagt aatacagcag ataagaacat ggcagcagat ttaacgaacg
     4321 aattaagccc tcactaaatg gcagatcgaa aatatttttt acagaggacg cttttaactg
     4381 ccctgacgcc acaccatcaa gcaattcggc aaagcgcgac ttgccattta ccagcacatc
     4441 cttaatgaac accatatggt tgaggccgaa cagatcgata gataaatcat cgctgtcttt
     4501 cagcatcaga acatcgcgaa taaacatctt catgccgatc ggaatattac acacgccgat
     4561 aaagcgttta aatccggtat gacgataaac ggcttcagtg accattcccg ccgggttagt
     4621 gaagttaatc acccatgcat tcggacaaag ttcttcgaca tcttttacga tgtcaaaaat
     4681 caccggaatg gtacgcagac ctttaaacag accgcccgcg ccgttggttt cctgaccaag
     4741 ataaccatga cttaatggaa tacgttcatc cagttcgcgc gccggtaatt ggcctacgcg
     4801 cagttgggta gtaacgaaat cagcatcttt caatgcttcg cggcgatcca gcgttttata
     4861 aagcttcatc gggacgccag cgttatcaat catccgttgg cagagttcaa aaatgatatc
     4921 cagtttctct ttaccatctt cgacatccac cagccataat tcgctgaccg gcaattcgtg
     4981 ataacgctta ataaatcctt ccagtaactc cggggtatag ctgctcccgc caccaatagt
     5041 gacgactttt aatttctggc tcataatttc tcccttcagt acagaatact gatatctggc
     5101 atatctgccc ccccggacat aaataatcca gcaacagtac agatatgtga attgtcaggt
     5161 ataacgactt actgattaaa ttcagtcaat ttcttacgat agctcttagg cgtaaaggac
     5221 gttaatttct taaacgtttt aataaacaaa ctcgggctac tataaccggc ctcaaacgca
     5281 atatccgtca cggaatagtt ggtcatttcc agttgttttt tggcaaaatt aatacggatc
     5341 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
     5401 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
     5461 ttatcatgca tcttttctac cgtacttttc agccactgcg gtacatcatc aatcacctgt
     5521 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
     5581 tccagccctg tttcgcggaa attcaatgaa gaaatcactg tttccacata ggtgagaaaa
     5641 gcgttattgg tccggtatac ctgcgaagcg acaaagcaat aaggcaacaa cggcaggtaa
     5701 tgctgctcaa aaaagcgttt actgatccca acgttcaata tacgcgtggc accaaactca
     5761 taaaaacttt gatggtgcga acctaacgga ataaaaacaa aatcgccccg ttccagtaac
     5821 acgcgcttac cgttaatctc ctggaaataa cgcccggtta ataccagagt aaattcataa
     5881 tagtcgtgct gatgcagccc gctgatactc tcagttttgt tatagataaa cacatggaag
     5941 tttttgccat taaacaactg ctgttctcgg gcagtggcaa tttccggcgc gttaatcact
     6001 ggctgcatca tcgactcctt atgccttcag tttttcatga agctcaatta attcagtaat
     6061 cagttcacgc gcaagcatgg acgtcattaa atgatcctga gcgtggacca gcaccagact
     6121 cactttcatc ttaccttcgc ccgcatcgcc ttcaatcagt ttcgtctgta ccagatgcgc
     6181 ttcattcaat gccatccgtg actgatccat catggctttt gctgcagcaa aatcgccctg
     6241 cttcgcctgt ttcagtgccg catacgccag gctgcgcgct tgtccggagt tgatgataag
     6301 ccccatcacc acttcttcca gctcttcagc ttctgtttgc gtatcaggaa tattatcgag
     6361 atccatcata catcgttcct ctctcttctt accggcaaga ttacccgtac cggcatcgat
     6421 taaaatttca gggcgttagc gatatcttct tcgctctctt ctttatcaat cgcattttgt
     6481 gctttgttag ccaccacaac aaagggcaga taaattaagg ttgcgatgcc aaggttgaag
     6541 agtgcgacca gcaatgcggc gacgctaccg ttggtgttaa agaaggctcc cagaccggtt
     6601 ggcatggtcc acggtgcaat attggtcacc ggaggaatga tgcccatgta gtacgctgcg
     6661 agggtgattg ccgccagaat cggttgtacc agtacaaacg ggataaacat caccgggttc
     6721 atgataattg gcagaccaaa cagaatcggt tcgttaatct ggaagatgcc ggacggcagc
     6781 gccagttttg ccacctgacg ataatcagca cggcgagagg cgataaagat agccaggatc
     6841 aggcctaaag tcgcaccact gcccccaagg aaaataaagg agtccagcat cggcttggcc
     6901 cagatatgga aggtcttacc ggctgccagc gccgcttcga cggaaccata ttgctgatag
     6961 gtcgcgatat tttccagcgc ccacggcgtc ataatgccgt tgtccagtgc ggtcagcgcc
     7021 agcgcgccat gaataccgaa gaaccagagc agtggaacaa agatcacata ggcccagccc
     7081 accacgctac ccaacgatgc cagtggggtt gagatggtat ccataatgat ctgatggaag
     7141 ttggtgcccc aggtattcaa cgcccaggca ataatcccca tcacggaaag aataataaag
     7201 ccgggaatta atgccgagaa ggaacgcgat actgaagcag gtacgctgtc gggcagttta
     7261 atgacccaat tgcggcggac aataaaggta aacatttctg ccaccaccag gccaataata
     7321 atcccggaga tgatattcgc cccacctaac cagtttgcgc caaccgcata ggcctcaccg
     7381 acactatatg gggtgacggt cataaatgct gcaacggata acaaccccgc cgccagcgca
     7441 tcgactttac gctcttctgc cagcgccatg ccaataaaga acggtgccat taaagacatt
     7501 atccctaatg ttccgttata tacgttgccg ccaatacctt tcaggccatt aagtgtttca
     7561 atggttgagg cgtcgaggcg aatacctaag gaataaaaaa acgacccctc cccaaagctt
     7621 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     7681 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     7741 aggagtacct tttcaagcga tgcaa
//
LOCUS       DAZGXZ010000199         7753 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000199 DAZGXZ010000000
VERSION     DAZGXZ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7753
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.199"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(150..560)
                     /gene="ybcV"
                     /locus_tag="ACHKJF_004858"
     CDS             complement(150..560)
                     /gene="ybcV"
                     /locus_tag="ACHKJF_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFP0952083.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            861..1067
                     /locus_tag="ACHKJF_004859"
     CDS             861..1067
                     /locus_tag="ACHKJF_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952084.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1640..2167
                     /locus_tag="ACHKJF_004860"
     CDS             1640..2167
                     /locus_tag="ACHKJF_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0952085.1"
                     /translation="MSNVSGIGDAYYWSVFKIAEAFGLHRDTVKKRLLAANTPVAATI
                     RGNPVYALQHVGPALFSVKHEAADSVHDPSRMEPKERKDWYQSENERIKLEKEQRKLI
                     PVDEVVIVYSSMRKAVVQVLETIPDVLERDCALTPQAVGVVQQAIDDLRYTLQEKSYE
                     ACAAELIPDEEGESL"
     gene            2176..3165
                     /locus_tag="ACHKJF_004861"
                     /pseudo
     CDS             2176..3165
                     /locus_tag="ACHKJF_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015987600.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            3330..4568
                     /locus_tag="ACHKJF_004862"
     CDS             3330..4568
                     /locus_tag="ACHKJF_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0952086.1"
                     /translation="MSVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWFAFKKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDA
                     VSDKNALQKKLISRLNEIMNYAVNCGTLKTNPLLKIKTAFTGKKNKSLAALPVERLPE
                     FLSWWDSVPHTYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHR
                     EHVVPLSSQAISILRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTPIAASKFKGIVTLH
                     GFRSMWSTLLNEEGFNPDVIEAALAHKSGDKIRDIYNRTIYLEQRKIMMQWVGDFFDD
                     ARKGVINRSGGMKGLRVVNG"
     gene            4582..4785
                     /locus_tag="ACHKJF_004863"
     CDS             4582..4785
                     /locus_tag="ACHKJF_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0952087.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            4845..5423
                     /locus_tag="ACHKJF_004864"
     CDS             4845..5423
                     /locus_tag="ACHKJF_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFP0952088.1"
                     /translation="MNKNIAVTGKGDARHVKKICDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCAALELTNSRTALQMLDDDEKGVNLTYTPGGNQNMSIISESGFYKLIARSR
                     KATTQGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRRKEQYQISASEKGRAL
                     QACKRKKRELEEEEKRLIREYQPEFYFGERIQ"
     gene            complement(5816..6043)
                     /locus_tag="ACHKJF_004865"
     CDS             complement(5816..6043)
                     /locus_tag="ACHKJF_004865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952089.1"
                     /translation="MTNNTLSPIQDTQTQDDEIIRQRQSEACARLEEELTRTKIPPPA
                     PRLVPPEKFALEDFVDKYPRRLKATKNRPPG"
     gene            complement(6101..6406)
                     /locus_tag="ACHKJF_004866"
     CDS             complement(6101..6406)
                     /locus_tag="ACHKJF_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0952090.1"
                     /translation="MSTSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVR
                     GEIARRQAEGRGENPLVSSLDALAQVEKIGVKAAEEIGQLVTIAREELQRRKTQESE"
     gene            6605..6802
                     /locus_tag="ACHKJF_004867"
     CDS             6605..6802
                     /locus_tag="ACHKJF_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0952091.1"
                     /translation="MVAVNHTPRLAHTQTAFVWRFIAVGASDRQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGSHHA"
     gene            6795..7262
                     /locus_tag="ACHKJF_004868"
                     /pseudo
     CDS             6795..7262
                     /locus_tag="ACHKJF_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            7252..7473
                     /locus_tag="ACHKJF_004869"
     CDS             7252..7473
                     /locus_tag="ACHKJF_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952092.1"
                     /translation="MHNKTTPDAAAAALTTLMHALIDIECTAGLAQKEEREEYTLFAL
                     ECIRYTATRSLNDAKNILVADCENGGGYA"
     gene            7466..7699
                     /locus_tag="ACHKJF_004870"
     CDS             7466..7699
                     /locus_tag="ACHKJF_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952093.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSMPELIRAAFFLLSTKEYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
BASE COUNT         2090 a         1624 c         1890 g         2149 t
ORIGIN      
        1 tattcaataa taaaatttat ccataaacct cgtttttacg gggttttgtt atatttgaat
       61 ggttccgaat atctaaatca caattgttga tggtttttat taaaccaatg cagtccggct
      121 caggagtgag agaagccgga cgttatggtt tagcgtggta agatctgtgt agttttctgg
      181 atgctttcag taaatagtaa tgagttatca aaggcatagt aatatctttg gtgttcctgg
      241 atatttgtaa cccatcggaa aactcctgct ttagcaagat tttccctgta ttgttgaaat
      301 gtgatttctt ttgatttcaa cttattatag gaggtctcta taagatgttt gtttctggag
      361 aatttaacat ttacaacctt tttgagtcct tttactaaca ctatgttgtc gttttctaac
      421 acaatgtgaa tattatctgt ggctaaatag taaatataaa gtgagacatt gtgacgtttt
      481 agctcagaat aaaataattc acagtttaaa tctttacgca cttgatcgaa tatttcttta
      541 aaaatggcag cctgagccat tggtaaacct tccatgtgat acgatggcgc gtagttagca
      601 ttatcgtgtt tattgtttca atctggtctg acctctttgt gttttgttga tgatttatgt
      661 caaatattaa gcctgttttt gatgaatagt gttgattgcg taacaaaatt gagccttgct
      721 ggcatccagg agggatatgc aaccgacaga tgtatgtaag gtcgatgtac tcaaactttc
      781 atacttttcc tcttttatgc agtaagattt gaagtaatat ttcaaccgct agatgacgag
      841 caaacgcatg gagcgacaaa atgaataaag aacaatctgc tgatgaactc tcgttggatc
      901 tgattcgtgt aaaaaatatg cttaatagca ccatttctat gagttacccg gatgttgtaa
      961 ttgcatgtat agaacatcag gtgtctctgg aagcattcag ggcaattgag gcagcgttgg
     1021 tgaagcacga taagaattcg aaggattatt ccctggtggt tgactgagca ccataactgc
     1081 taatcattca aactatttca cctgtgacag agtcaatatc gcattctgtc actgtcaggc
     1141 taatacagag ctgcaattca actactgcaa tgtcctcgta attaggtgaa tttacaatat
     1201 cgtcctgttc ggatgccggt tgcattgctg aagatgaggc atttatggtt cgcatatttt
     1261 cccctcatgc tcgtcagtcc tgtgcgtagg aagaaacagg acactcacac taatttgtgt
     1321 gggcatgctg tgatgtcctt ctgaattatt cctatgccat tatgtaaagc gctgtatcag
     1381 atgctcatca cggctgtcag gctgtcaggc tgtcgggtcc tcccggtggg ggcccctgcc
     1441 acggggcggg agcgtcgcgg aaaaaggcta gtttttgcat ttccatggcg gcggcagcat
     1501 gtttggtaat ttattgataa ttaaaagtta tttctctttt cacctgtaca atattttttt
     1561 ctccctgtca ttagaccagt ttgcaattaa ttgaaatata taaataaaca tgattttcac
     1621 ctgccagatg gagttgctta tgtcaaatgt gagcgggatc ggtgatgctt attactggag
     1681 tgtttttaaa atcgctgagg cctttgggct tcaccgggac acagtaaaaa aacggctcct
     1741 cgcggccaac actcctgtgg ctgcgactat cagggggaac cccgtttacg ccctgcagca
     1801 tgtcgggcca gccctgttta gtgtgaagca tgaggcagca gactctgttc atgatccatc
     1861 ccgtatggag ccgaaagaga gaaaggactg gtaccaatct gaaaatgaaa ggatcaagct
     1921 ggaaaaggag cagcgaaaac tcatccccgt tgatgaagta gtcatcgtct attcgtccat
     1981 gagaaaggct gtcgtccagg ttctggagac aattccggat gttcttgaac gcgattgcgc
     2041 cctgactcct caggccgtcg gcgttgtaca gcaggccatt gatgacctgc gatacactct
     2101 tcaggaaaaa tcctacgagg cttgtgctgc tgaattaatt cctgatgagg aaggagagag
     2161 tctctaggag gaataatggg tttttcatca gcccgaaatt tgggaaggga catatcggca
     2221 ggattttccc caccacgtcg catgccgatt tcggaggctg ttaaaaaatt catgcgtgtt
     2281 cccaaggggg ctggtaactc ggtgccatgg gatcctgaac tgacacccta catcattgag
     2341 cccatgaact gcctggcatc gcgtgaatac gatgcggtga tttttgttgg tcctgcgcga
     2401 acagggaaga ccattggtct gatcgatgga tggattgtct ataccatcgt ttgcgatcct
     2461 tcggacatgc tcgttgtgca gatgaccgaa gataaggccc gcgagcattc taaaaagcgc
     2521 ctcgacagaa cgttcagaag cagtgcggcg gtaaagaaaa gaatgagtcc acgtcgtaac
     2581 gacaataatg tccatgataa gacgttcagg gatggctcgt tccttaaaat tggttggccc
     2641 tcggtcaaca ttatgtcgtc gtcggattac cggtttgtcg ccttaaccga ttacgaccgt
     2701 tttccggaga atatcgacag cgagggtgat ggtttctccc tggcctcaaa acgtaccacc
     2761 acatttatgt ccgccgggat gactctggtg gagagctcgc cgggacgtga catctgcgac
     2821 agcaaatggc gacgtaagtc gcctcatgaa gcgccaccga cgactggtat tctttccctt
     2881 tacaatcgtg gtgaccgccg ccgctggtac tggtcatgtc cgcactgcgg tgaatatttt
     2941 cagccagcca tggatgccat gaccggctac cgtaatgaac cggatccctt taaagccagt
     3001 gaggcggcgt atctactttg cccgcactgc agcggcatta tcactgcgga gaaaaagcgt
     3061 gagctcaata gtgcaggagt ctggttgcgt gaaggtcagg tcattgatcg taacggcaac
     3121 gtttccggtg aaccgcgccg ctcccgtatc gccagtttct ggatgtaggg tacatttctt
     3181 acctgtttgt atgttctggt gtcgtttcat agtcttttca atgagttgtg attttatgag
     3241 tttcctctct ttgcttgata atgagttagt ttatcgcttg ttattggctt gaatggacta
     3301 catgacggac taaaaaatga gggcgataga tgtcggtaaa gccattaacc gtgactgaag
     3361 ttaaggggat gaaaccacgt gaaaaggact atgccgttta tgatgggttc ggtttattgc
     3421 tgaatgtgag taaagccggt gggaaagtgt ggcgtttccg ttatagccat ccgataacga
     3481 agaaacggca gacatacacg ataggacgtt ttcctgaatt ctcactcgcg gaagcacggg
     3541 aagtacgtga tgaacttcgg cgaatgattg cacgtggagt tgatccagtg acggagaaga
     3601 aaaatcgtaa aattgagatg tcactaaaaa atctacagac atttgaagct gttgctaatg
     3661 catggttcgc ttttaaaaag ggatctgaat tgcggaaacc tacgctgtat aatatcgaat
     3721 atgaagtata caaatatctt gttcctttct ttggtaagta cagtatagaa aaaattacag
     3781 caccagtagc tattaatgct ctggatgccg tatccgataa gaatgcgttg caaaaaaaat
     3841 taatatcaag attaaatgaa attatgaatt atgctgtaaa ttgtggaaca ttgaaaacaa
     3901 atccattact taagataaag actgcattca caggaaagaa aaataaatca ttagcagcac
     3961 tacctgttga aagattgcct gaatttctga gctggtggga tagtgtgcct catacctatc
     4021 aaatagctca taatgcactt ttattccaga tattgacaat ggtcaggcca ggtgaggcga
     4081 ttaaagcaga gtggtcagag attgattttg attctggctt gtggattatc cccgcgcata
     4141 aaatgaaatg ccatcgtgaa catgttgttc ccttgtcatc acaggctatt agtatcctca
     4201 gaacaatgca ggaaataaaa agagggcgtt atgtgttttt ttcctccaga acaaaagatg
     4261 cgcctatggg gaggaatact atcaagaccc caattgctgc cagcaagttc aaagggattg
     4321 taacgttaca tggttttcgt tcaatgtgga gtacgctttt aaatgaggag ggatttaacc
     4381 ccgatgtaat cgaggctgca ttggcgcata aaagtggtga taaaataaga gatatttata
     4441 atagaactat ttatctagaa cagcgtaaga tcatgatgca atgggtcggt gatttttttg
     4501 atgatgcgag aaaaggggta attaatagat ccggtggtat gaaaggttta agagttgtaa
     4561 atggttgagg aggttcagca aatgaatacc aatgaagata ttttatttac taaagacgta
     4621 atgaaaatct tgcgatatgg agcaatgagt gcattcatca atttctggaa agatgagaat
     4681 aatggttttc ctcagccgtt cagaattgga cgacgacata cctggcaccg tagagatgta
     4741 gaagcatggt tagataaaca acgagaacaa gccaatcccc actaataata tctttcatac
     4801 cccgcgtgca atgcggggtt ttttgtatgt gaggtaaaaa agaaatgaat aaaaatattg
     4861 ccgtgacggg caagggtgac gcacgtcatg taaaaaaaat ctgtgatttt cgtgatctgg
     4921 tcgttctgcg ctttgatagt gtgaacgttc gcgtggtgta tctgaacggc gatccgtggt
     4981 ttgttgcaaa ggatgtctgc gctgcgctgg aactgaccaa ttcgcgtacg gcgttgcaga
     5041 tgcttgatga tgatgaaaag ggagtaaatt taacttacac cccaggagga aatcagaata
     5101 tgagcattat ctctgagtca ggtttctaca aactaatagc ccgtagccgc aaagcaacga
     5161 cgcaaggcac attcgcccat cgtttcagta actgggtatt cagaaatgtg ataccgggta
     5221 tcagaaaaac gggggcttat ggtatcccgt ggggtgcatt acaggatttt tcccgccgca
     5281 aagagcaata ccagataagt gccagcgaga aggggagggc gctacaggca tgtaagcgca
     5341 aaaaacgtga actggaggaa gaagaaaaaa ggctgatacg tgaatatcag cctgagtttt
     5401 actttggtga gcgtattcag taaccacacg cggtgctgat tatacggtac atcgtgttaa
     5461 ccgagaagct acccaccagc aaggcaaaat cttctgctaa aaaatgacat atggcccgtc
     5521 gtccggaaag catgaaattt tacaaaaatg gaaaatgaag atttttattg tgctggtggg
     5581 taaaaacaaa aagcgccccg ttgccggagc gcccttgcga acaattaacc tgctgcgaaa
     5641 aaattggatc agtgcagggg aattatatca actgtgtgaa gaagcgccac aattgccgga
     5701 taacaggcaa agaaaaggcc acctgtcacg gtggcccttc gacacaaatt tcgcgttatc
     5761 cccaacgcat gagcatcgcc gacaatgcca catttacggc tggtggacaa ggctatcagc
     5821 caggcggtcg gtttttagtg gcttttaacc gccgtgggta tttatcgaca aaatcttcaa
     5881 gggcaaattt ttctggtggc actaagcgcg gcgctggggg tggtattttt gttctggtta
     5941 gttcttcctc aagtctggcg caggcttctg actgcctttg ccggatgatt tcatcatctt
     6001 gcgtttgcgt gtcttgtatt ggtgatagtg tgttgttggt catgatactg ccctgtaaag
     6061 caatgcgccg gagttcctca cgccacggcg ctgatagtta ttattctgat tcttgggtct
     6121 tgcgacgctg gagttcttca cgcgcgatgg tgacgagttg cccgatctcc tcggctgctt
     6181 tgactccgat tttttccacc tgcgccagtg catcgagcga agaaaccagg gggttttctc
     6241 ctcttccttc tgcttggcgg cgggcgatct caccgcgtac tgctgtaact ataaaattcg
     6301 ctttagtttc ccctggctgt agggatgcct caatgccact cactacttca tgtggaaatc
     6361 tggcattcag ttgctgtgac ttattgttaa cagaactcgt ggacatgttt gcacctccga
     6421 ttaatagcgg gatgcagtgt aacacaaaag tgaataccca aaaaagcttg aaatggtatt
     6481 cacttaaatg taatctgaat accacaagag ctgatacatg caattcagca attgaaacga
     6541 cgaagccccg cactgttgga gcagtaacgg ggcttctaac caacaacgta aacgaggagc
     6601 cgttatggtt gccgtaaatc atactccacg actagcacac acacaaacgg cctttgtgtg
     6661 gcgctttatt gccgtcggcg cgtcagaccg tcaaatcatc cacgtaaccg cctggacgga
     6721 acgcgaagcg cgtagccgtt gcccgtccgg ttgtgttgct gtatttgccg ctcgtattcg
     6781 tcaggggtca catcatgcgt aaaaaccgct tgcaaaaaat tatcacgggg ctgtatgctt
     6841 cacccgtcgc ccacatggcg accgggttta gcagcctgaa taaattggcg gacagccgcc
     6901 catatccgat aagcggtttt tttgtgtccg taaacctacc catacccgca ttatggcggg
     6961 gcgtaacggg ggagcctttg cgctcgctgg tttcccaatt tgccagtctg ctaaccctgt
     7021 tacgtctcgc caccccgttt agcagcgtag tggtgagacc ccttaacatt aaattgggag
     7081 cctttcacat ggctgtatcc gcacgccctt actttgtctg gcgctttatg cagtgccacc
     7141 acagcagcat tgatatttac accgttaccg cagccactga acgcgaagcc cgcgcccagt
     7201 tgccgcgcgc acatctgatt tttgtcgccc gtattcgtca ggggaaaacc tatgcacaat
     7261 aaaaccacac cggacgcagc cgccgccgcg ctcactacgc tgatgcacgc acttattgat
     7321 attgaatgca cagcagggct tgcacaaaag gaagaacggg aagaatacac attgttcgcc
     7381 ctggaatgta tccgatacac cgccacgcgg tcgctgaatg acgctaaaaa tattcttgtt
     7441 gctgattgtg aaaatggggg gggttatgcg tgatgatcgt tttaattccc tgaaacaaga
     7501 attttccggc gttcctgatg atgcggctga tgcgctttcg tcaatgccag aacttattag
     7561 agcggctttt ttcttacttt ccacgaaaga atataaatca acgggacttg atgtactgaa
     7621 tatcgccgcc gattatgcag aatatgtggc agaggcgcgt tacagaagaa aatttcctga
     7681 ggatgtaagc catgcgtgat atttatcttg aaacaataga ccgcgcatca cttgcacttt
     7741 ctcacagtga aaa
//
LOCUS       DAZGXZ010000200         7747 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000200 DAZGXZ010000000
VERSION     DAZGXZ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.200"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..821
                     /locus_tag="ACHKJF_004871"
     CDS             <1..821
                     /locus_tag="ACHKJF_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0952094.1"
                     /translation="TVAVLVPSLTDKASSRFMQSLQQVLNKNEFQLLLGCHEYNQLKE
                     TEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVVGSPFSPAQITIETAFFE
                     ASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQNTDLVITMPDAAS
                     LQLGRYALNEILLRQPELDAVICSHEEIVLGIMFECQRRLLKIPGNIAVACLDGSDNC
                     DQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNHDESEESRIVQFNYQIELRQST"
     gene            1218..1487
                     /locus_tag="ACHKJF_004872"
                     /pseudo
     CDS             1218..1487
                     /locus_tag="ACHKJF_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1718..1816)
                     /locus_tag="ACHKJF_004873"
                     /pseudo
     CDS             complement(1718..1816)
                     /locus_tag="ACHKJF_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase IV"
     regulatory      1937..2125
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2190..4034
                     /gene="btuB"
                     /locus_tag="ACHKJF_004874"
     CDS             2190..4034
                     /gene="btuB"
                     /locus_tag="ACHKJF_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP0952095.1"
                     /translation="MIKKYWFLMVYSVTAFSVWAQDTPLDTLVVTANRFQEPLSTVLA
                     PVTIVTREDIDRWQVSSVNDVLRRLPGVAISQHGGEGQLSTIFVRGTNSNHTLVLIDG
                     VRLNLAGVSGAADLSQFPVALVQRIEYIRGPRSAIYGSDAIGGVINIITSRENTGTEI
                     SAGWGSNSYQHYDISTHQQLGENTRVTLLGDYTYTRGFDAVAYGSTGMQPQSDRDGFL
                     SKTFYGKLEHNLSDTWSGFVRGYGYNNRTKYDAWYSPGSPLIDTRKLYSQSWDAGLRY
                     AGETLQSQLVSSYSHSKDYNYDPHYGRYDTSANLDDMKQYNLQWTNSVTVGHGNVGAG
                     IDWQKQSTTPGTGYLPKGYDQRNTGIYLTGLQKLGDFTLEGAVRNDDNSQFERHTTWQ
                     SSAGWEFIEGYRFIASYGTAFKAPNLGQLYGMYGNPNLAPEKSKQWEGAFEGLTGGVN
                     WRISGYRNDISEMINYNPHTLRYYNDGKVHVKGIEATVNFDTGALTHTVSYDYTDARN
                     ALTDKPLERRPKLQVKYQLDWQVFDFDWGITYQYMGSRYDSDYSSWPYKSVKMGGVSL
                     WDVAVAYPVTPHLIVRGKIANLFNKDYETGYGYQAAGREYILSGSYTF"
     gene            4048..4239
                     /locus_tag="ACHKJF_004875"
                     /pseudo
     CDS             4048..4239
                     /locus_tag="ACHKJF_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4380..6467)
                     /gene="iha"
                     /locus_tag="ACHKJF_004876"
     CDS             complement(4380..6467)
                     /gene="iha"
                     /locus_tag="ACHKJF_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP0952096.1"
                     /translation="MRITTLASVVIPCLGFSASSMAAEDVMIVSASGYEKKLTNAAAS
                     VSVISQEELQSSQYHDLAEALRAVEGVDVESGTGKTGGLEISIRGMPASYTLILIDGV
                     RQGGSGDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITKKNA
                     DKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQIRGSTQQRQGSSVTSLS
                     DTSATRIPYPTESQNYNLGARIDWKATEQDVLWFDMDTTQQRYDNQDGQLGSLTGGYD
                     HTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKSDKWGLAGKPREL
                     KESNLILNSLLLTPLGESHLLTVGGEYQSSSMKDGVVLASTGETFRQKSWSVFAEDEW
                     HLTDVLALTAGSRYEHHDQFGGHFSPRAYLVWDVTDAWTLKGGVTTGYKAPTMGQLHK
                     GISGVSGQGRTNLLGNPELKPEESVSYEAGVYYDNLSGLNANITGFITDFSNKIVSYS
                     IDDNTNSYTNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTQSEQRDGDNKGAPLSYT
                     PEHMVNAKLNWQITEEVASWLGARYRGKTPRFTRNYSSLSAVQKKVYDEKGEYLKAWT
                     VVDAGLSWKVTDALTLNAAVNNLLNKDYSDVSLYSADKSTLYAGDYFQTGSSTTGYVI
                     PERNYWMSLNYQF"
     gene            6406..6588
                     /locus_tag="ACHKJF_004877"
     CDS             6406..6588
                     /locus_tag="ACHKJF_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952097.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     IYLLQKMNITDKTKYK"
     gene            7008..7201
                     /locus_tag="ACHKJF_004878"
                     /pseudo
     CDS             7008..7201
                     /locus_tag="ACHKJF_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
     gene            7299..>7747
                     /locus_tag="ACHKJF_004879"
     CDS             7299..>7747
                     /locus_tag="ACHKJF_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0952098.1"
                     /translation="MIVCYVILVNRPFETEIAGKKLFFQKNDVLLTDNISQSFFLRHP
                     GNVTELRIDECIVRKYMESADRIQHSDSAVSVPFIKTTFSHIALLHNVMQNLSLQKTL
                     PQQFSEQMFFACLSIFSTHRGFMFLLFRCINSVALKVRYIICSSPSRA"
BASE COUNT         2086 a         1793 c         1781 g         2087 t
ORIGIN      
        1 atacagtcgc cgtactggtt ccttccctga cagataaagc cagctcccgc tttatgcagt
       61 cgttgcagca ggtgcttaat aaaaatgaat tccagctact gcttgggtgt catgagtata
      121 atcagctcaa agaaactgaa attctgatga cgcttcttca aggcaacccg gcggcgttag
      181 ttatttttgg ttctcagttg gctgataaaa ctcaccagat actcgagaaa acaaatatac
      241 ctaccattaa cgttgtcggt tcccctttca gtccggctca aattaccatc gaaacagcat
      301 tttttgaagc atcacataaa ctgaccgaac atctgctgga gcagggttat aagaatatcg
      361 gatttatcgg tgcgcatatg gacaaccgcc tgcaacgtca gcaactgaac ggctggcata
      421 aagcaatgct gagccattac cagaacactg atcttgttat caccatgccg gatgccgcaa
      481 gtctgcaatt ggggcgttac gcattaaacg agatattact gcgacaacca gagctggatg
      541 ctgtgatttg cagccacgaa gagattgtgc tgggtatcat gtttgagtgc cagcggcgtt
      601 tgttaaaaat ccctggaaat atcgctgtag cttgtcttga cggttcagac aattgcgatc
      661 aaacacaccc aactttgaca tccatccgta ttgactataa aaaaatgggg acagagacgg
      721 gtaagctgct gattggacta ttgaataata atcatgatga atcagaagag tcgcgtatcg
      781 tgcaattcaa ctaccagata gaattacgtc agagtacatg agcctaaaag agcactatta
      841 caaagttagc tattgtggta gggatagccg tttccggcta ttccctgcgc agttccggat
      901 gtatgctgct aacgcatcca ttcctacttc aggtatttgg cgtagaagcg gatgctaaaa
      961 acttgccgca aaaccgcaat atcagcctgc agtatcgctc tccagattat ccattcctat
     1021 attgatgaca acgacaggta agaccgtgac ctgataaacc tgaataagct cctggacata
     1081 aagtccgtaa tgggaataag gcgagagtca gcgaatctca tcgaggccag cagcactggc
     1141 tgcaataagg atatagagca gcaatctacc gtcaacgtca aaaacacgga agccttgcaa
     1201 acgggatgcg ttcatataaa caacatggag cagcaacaat acgatgcctg tcgttgctca
     1261 gtcaggacct tttctgtcat gcgcgagcca gcgtcacgat tgataaataa tgttagcaac
     1321 tgattaattt actgcaacag acgtggttgg taataagcac tggcggacaa tgcaggatgg
     1381 aaggctgttt tcaggttgtc cgttcaggta cgttagatca gttcgttgcg ctaaagacaa
     1441 ctgttcagtc ccttatgtta cccgttatgg caaccattgc ccggtgataa ccgggcgtgt
     1501 catctgtgca tccgttgatt cgtgtggact gataaagttt tgtctgctga atctttatac
     1561 ggaatgctct tcagtatgcg taagataatt tgttttttgg aggattgtcg acaatcagag
     1621 ggtaaacaaa agaatggttg aatgtggatg aggttcatat tattgattca gattttttga
     1681 agaatcacca catgggatgt ccatgtggtt gtataactca gggccagcgc ttaataatga
     1741 tggatgtatt aatgccgcca aaggcaaagt tgttactctg caaaaacaca caatcaacct
     1801 tgcgggcttc atgcaactaa ttgagcatat atgttttatt cttattatgg tcgccgataa
     1861 aatattatgt gtattagatc cgagtgttca tgcattccta tttttgagtc atgtatggac
     1921 agtaacttgt tacaaccagt aatatccagt tgtcggtcct gcgagttaat agggaatcca
     1981 gtgcaaatcc gaaactgaca cgcagcggta aggaaaggtg cgatgattgc gtaatgtgga
     2041 cacggtcatc cggtgggtgg tcatctcgtt gtatcttcag atgcccttct aggtctgaag
     2101 acctgtcggt taacgtcgca tctggttctc atcatcgctt actatcgatg gtaactgcga
     2161 tattcttctt ctgttgtgga tgcgttacaa tgattaaaaa atattggttt ctaatggtgt
     2221 actccgtcac agccttttct gtctgggcac aggatacccc tctggatact ctcgttgtta
     2281 ctgctaaccg ttttcaggaa ccattaagta ccgtgcttgc tcctgtaact attgtgacac
     2341 gagaagatat agatcgctgg caagtttctt ctgtaaatga tgttctgcgt cgtttgcctg
     2401 gcgttgcaat ttctcagcat gggggagaag ggcagctctc aacaattttt gttcgtggga
     2461 caaactctaa ccacactctg gtattgattg atggagtgcg cctgaattta gcgggtgtca
     2521 gtggagctgc agacctgagt cagtttcccg tcgctttagt gcagcgtata gaatatatcc
     2581 gtgggccacg ttcagcaatt tatggttcag atgccattgg cggagtgata aatattatta
     2641 catcccgtga gaatactggc actgaaattt ccgcaggatg gggaagtaat agttatcaac
     2701 attacgatat atccactcat cagcaacttg gggaaaatac ccgtgtaaca cttcttggcg
     2761 attataccta tactaggggg tttgatgctg ttgcctatgg cagtacaggg atgcaaccac
     2821 agtcagacag agacggtttt ttaagtaaaa cattctatgg caaacttgaa cataacttat
     2881 ctgatacgtg gagcggattt gttcgtggct acggctataa taaccgaact aaatatgacg
     2941 cctggtactc tcccggttct ccactgattg atactcgaaa actttatagt caaagctggg
     3001 atgcaggtct tcgctacgct ggtgaaacac tgcaatctca acttgtttcc agttatagcc
     3061 atagtaaaga ttacaactac gatccacatt atggacgcta tgacacatca gctaacctgg
     3121 atgatatgaa acaatacaat ctccagtgga caaatagcgt cacagtcggc cacggtaatg
     3181 ttggcgctgg tatcgactgg caaaaacaat ccactactcc tggaactgga tatctgccaa
     3241 aaggttacga tcagcgtaac acggggattt atctgacagg actgcaaaaa cttggcgatt
     3301 ttacgttaga aggcgcggtg cgtaatgacg acaactcgca atttgaacgc cacacaacat
     3361 ggcaatccag tgcgggatgg gagtttattg agggttatcg ctttatcgct tcctatggaa
     3421 ctgctttcaa agcgccaaat ctggggcagc tatatggtat gtatggtaat ccgaatctgg
     3481 ctccggaaaa gagtaaacag tgggaagggg catttgaagg tttaactggg ggcgtaaatt
     3541 ggcgtatttc aggataccgt aatgatatca gtgaaatgat taactataat cctcataccc
     3601 ttcgttatta caacgacgga aaagtacacg ttaaaggaat tgaggctaca gtaaactttg
     3661 atacaggagc actgacgcat actgttagtt acgattatac ggatgccaga aatgccttaa
     3721 ctgacaaacc attggaacgt cgtccaaaac tgcaagtgaa gtatcaactt gactggcaag
     3781 tattcgattt tgactggggg ataacttatc aatatatggg atcgcgatac gattcggact
     3841 actcatcctg gccctataag tcagtcaaaa tgggcggtgt gagtttatgg gatgttgcgg
     3901 ttgcgtatcc ggtcactcct cacctgatag ttcgcggtaa aatagctaat ctgttcaaca
     3961 aagattatga gactggttat ggctatcagg cagcaggacg ggaatatata ttgtctggca
     4021 gctatacttt ctgaaatctg ccccaccgag tcgctaaaca gcgtgatccg ccatgcgatc
     4081 aaaaagcgta aagtgttccc gacagacgag gcggtgaaaa aagttgtgtg gctggcaatc
     4141 cagtctgcgt cccagaaatg gacgatgccg ttgaaggact ggcgtatgga aatgagccgc
     4201 tttattatca agttcagtga ccgcctggac ggtcacttct gggaaaaggc atttacacag
     4261 aatcctgaac aggctcggtc acttcgacca gacaccgtgc gtcatgtaac gttgccactg
     4321 ttccgccagg agttacagtg gcaacgtatg ctaccgtcag tgatggcgtt ttgttgttat
     4381 cagaactgat agttcagcga catccagtaa tttcgctcag gtatcacata tcccgttgtt
     4441 gatgatcccg tctggaagta atcaccggca taaagcgtac tcttatcggc actgtacagg
     4501 ctcacgtcac tgtaatcctt gttgagcagg ttattcaccg cagcattcag cgtcagggca
     4561 tccgtcacct tccacgacag acctgcatcc accaccgtcc aggctttcag atattctcct
     4621 ttctcatcat atactttctt ctgtacagcg ctcagcgacg aataattccg ggtgaaacgt
     4681 ggtgttttcc cgcgataacg ggcacccagc catgatgcca cctcttcggt gatctgccag
     4741 ttcagtttcg cattcaccat gtgttcaggg gtataactca gcggtgcacc tttgttatca
     4801 ccatcacgtt gttcactttg ggtccaggtg taattcaacg acagagtgac atcctctgac
     4861 cacagcggca atgtgccggc gaattccaca ccatgcaagc gggcctttcc gctgtttgta
     4921 tagctattgg tgttatcatc aatggaataa gagacaatct tgttggagaa gtctgttata
     4981 aagcctgtta tattggcatt cagaccggag aggttatcgt aatatacccc cgcctcataa
     5041 cttacactct cttccggctt cagttcgggg ttaccgagta gattagtacg tccctgcccg
     5101 gatacaccgc tgatcccctt atgtaactgt cccatggtgg gggctttata tcccgtggtc
     5161 acaccgcctt tcagcgtcca ggcatcggtc acgtcccaga ccagatatgc acgcggactg
     5221 aagtgtcccc cgaattgatc atgatgttca tagcgactgc ctgcagtcag cgcaagtaca
     5281 tccgtgagat gccactcatc ctcagcaaat actgaccagc ttttctgtcg gaaagtttca
     5341 cctgtgctgg caaggacaac cccatctttc atggacgaac tctgatactc tccccccaca
     5401 gtaagcagat gagattctcc cagaggggta agcagcaatg aattcaggat aaggttagat
     5461 tccttaagct cccgcggttt accggcaagc ccccatttat cgctcttcag aacactacgt
     5521 acaagctcac gacctttatt ttccgtctcg ttccagttca gatacgattt ccaggtaccg
     5581 aaggtgaaag tatgatcata gccagccgaa attttgtttc gctcatagcg cagagtatgg
     5641 tcatatcccc ctgtcaggct acccagttga ccatcctggt tatcgtaacg ctgttgggtg
     5701 gtatccatat caaaccagag cacatcctgc tccgtagcct tccagtcaat acgcgcacca
     5761 agattataat tctgtgactc cgtgggataa ggaatacgcg tggctgatgt gtcgctcagt
     5821 gatgtgaccg atgagccctg acgctgctgt gtgctgccgc ggatctgcag actgacagaa
     5881 tcatccacaa gagggccact gctccagaaa ttaaactggc tgctgttgcc ccatttgtta
     5941 ctctcctgca gattcagccc ggcgttgacg gaagagagcc atttgtctgc attctttttg
     6001 gtaatgatat tcaccacacc acccatcgca tcagagccat acagtgtgga catcggcccc
     6061 ctgataacct caatacgctc aatcgcggcc agaggaggca tgaaccctgt attcatggca
     6121 gaaaaaccgt taggcgtcac gtccccgctc ccgccctgac ggacaccatc aatcagtatc
     6181 agcgtgtaac tggcgggcat tccacggatg ctgatttcca gcccaccggt tttacccgta
     6241 ccactttcaa catccacacc ctccacagct ctcagtgcct ccgccagatc gtggtactgg
     6301 ctggactgca attcctcctg actgattacg gaaacactgg cggctgcgtt ggtcagtttt
     6361 ttctcatagc cggatgccga gacaatcatc acatcctctg ctgccatgct gctggctgaa
     6421 aacccgagac agggaatgac aacggaagcc agagtggtta ttcgcatatt actaatctcc
     6481 gatcatgtta atcggcaaga caatacacaa atacaaacaa taatgagaat aatttatatt
     6541 taccttttac aaaaaatgaa tattactgac aaaactaaat ataaataatg actgcctcgg
     6601 catgccagtt aacagccatc cgcaatggcg tgttcgattt tgcgaataaa aaatggttac
     6661 tgtagaagcg gattttcacc acctgtcaac catgaatggt cagtggagtg agacgactgg
     6721 tatgatttgg tcgatgactt cttccggtga atacggacag aaacattgaa atattgtaag
     6781 agagaacgtt cagaattctg tgtcacgtga aaatactaca gcgttatcac cttattcagt
     6841 cgaccttcaa aataaacgtc taaccgtgac aatcaccatt tggattgccc actggagagg
     6901 cgaacgattg cctcgacggc atttataatt ccagcccaca ttgttggcgc tgacaatact
     6961 aaggacaaac gatgagggag aaccgtctta taccactagc gctgacaccg gcacaccttc
     7021 cccgtgaaat acaccggctg gagcctgaag aaagctgttg cccggagtgt ggcggtgagc
     7081 tggattatct ggggaagtca gcgctgaaca actggaactg gtgagcagct ccctgaaagt
     7141 gatcagcaca gaacgggtaa aaaaagcctg taaaaaatgt gacggtaccg ttgaagcgcc
     7201 ggtctcgtaa gaataatcct gggcaaataa gatttttcct gaaaaagcgt ctagtctttt
     7261 gatatcgttg tttttatgct aatcagggag tatatgtgat gattgtctgt tacgttattc
     7321 ttgttaaccg tccgtttgaa acagagattg ccgggaaaaa gctatttttt cagaaaaatg
     7381 acgtcctgtt gacagataac atcagccagt cctttttcct gcgccatccc ggtaatgtta
     7441 ctgagctccg gattgacgaa tgcattgtca gaaaatatat ggaaagtgcc gacaggatcc
     7501 agcactctga tagtgctgtt tctgttccgt tcattaaaac aaccttctct cacatcgctc
     7561 tgttacataa tgtcatgcag aatctcagtc tgcaaaagac attgccacaa caattttctg
     7621 agcagatgtt ttttgcctgc ctttccattt tttccacaca cagaggtttc atgtttctgc
     7681 tattcagatg cataaactct gttgcgctta aagtcaggta tattatctgt tccagtccat
     7741 cacgggc
//
LOCUS       DAZGXZ010000201         7644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000201 DAZGXZ010000000
VERSION     DAZGXZ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.201"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            107..430
                     /locus_tag="ACHKJF_004880"
     CDS             107..430
                     /locus_tag="ACHKJF_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952099.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
     gene            complement(491..2593)
                     /gene="mchF"
                     /locus_tag="ACHKJF_004881"
     CDS             complement(491..2593)
                     /gene="mchF"
                     /locus_tag="ACHKJF_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFP0952100.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIFRITN"
     gene            complement(2586..3860)
                     /locus_tag="ACHKJF_004882"
     CDS             complement(2586..3860)
                     /locus_tag="ACHKJF_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0952101.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            complement(4013..4465)
                     /locus_tag="ACHKJF_004883"
     CDS             complement(4013..4465)
                     /locus_tag="ACHKJF_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP0952102.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPLAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            complement(4491..6041)
                     /locus_tag="ACHKJF_004884"
     CDS             complement(4491..6041)
                     /locus_tag="ACHKJF_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFP0952103.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTAYAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSARKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVHFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            complement(6313..6540)
                     /gene="mchB"
                     /locus_tag="ACHKJF_004885"
     CDS             complement(6313..6540)
                     /gene="mchB"
                     /locus_tag="ACHKJF_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFP0952104.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(6568..6777)
                     /gene="mchI"
                     /locus_tag="ACHKJF_004886"
     CDS             complement(6568..6777)
                     /gene="mchI"
                     /locus_tag="ACHKJF_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFP0952105.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            6886..7122
                     /locus_tag="ACHKJF_004887"
     CDS             6886..7122
                     /locus_tag="ACHKJF_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952106.1"
                     /translation="MIKDNSKVDDSADRLSFTETLFVAHSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
BASE COUNT         2270 a         1766 c         1420 g         2188 t
ORIGIN      
        1 gacacattaa ccctttatct tttcataaag aaaataattc tttcgttact tgcgagtgta
       61 ctgtttttat ttgttttatc tttctttaat aaaaagagga aataacatga gaaatataac
      121 aaccgatgaa cttgctttaa tacatggtgc aggattagat aaagatgcat ccgttgcaat
      181 aggtactgcg gctggtggtt ttttaggtaa agcaacaaaa atccctggtg cggatatagc
      241 aggaagtgca attggaggat atattggagg agttattgct gatggttcaa ataggacaat
      301 aacaatacca tcaaacggta ttaactataa tccgggtata ggacttggaa gttttaatcc
      361 aaactacaat ggaggtttat taaactcagg atttaactca tcaacatcct cttctagtag
      421 tggaagttga ttaatttcta aagcagcata gcttattatg cagcaatgct gcttttagtg
      481 atatattatc ctaatttgta attctaaaaa tccgatcaac tgttcttaaa gttgtctctc
      541 tatgtgcaat aatcacccgg gtgatattca tcttttttat agcagcattc acgaaacgtt
      601 cactttctgt atcaagagaa cttgtagcct catccataaa taatattcca ggtttccggt
      661 ataaagctcg ggcaatgaat atacgctgtt tttgaccgcc ggaaagacct tcccccagtt
      721 cacctattaa cgtttcatac cccattggca ttttcattat cacatcatga atatgacttg
      781 ctttggcaca ttctgtcatc cattcgtcgt ctgtttcttc tgcaaaccca cagatatttt
      841 cacgaattga tcctgaaaat agccggtcgt cctgcataac acaggctatc atacggtgat
      901 aattatttat tccaagttga cgtatatccg tgccattaac cagtactttt ccactatctg
      961 gttcaaacag tccacataat actttcatta atgtggtttt tccggcaccg gaggcaccag
     1021 ttatagccac actttctccc ggagccacag acaaattcag accactgaat acctgtgctg
     1081 actggctgtc ataccggtag cttaaatcag tggtttccag tgaaaccggg ctcatgtcag
     1141 caacgatttc aatttccggt ttcttttctt ccttttcatg tagtgcaata tctgcaatgc
     1201 gctcattatg cagactcatt attctcagtt gaagaagaaa actggtcagc gaagcaaccc
     1261 gatccgaaaa ctgcccacga aaagaaccaa atgccacaaa catccctatt gtcatctgat
     1321 tatcgatcac aaggcttgca cccagccata aaatcgccac ctgatcacag gcggcaacaa
     1381 aagtatttat ccccccgaag agcaaatcca tcctggttaa cttaatacct gaattgatcg
     1441 catctatttt caggttaagc cagtgtgttc cccgaatccc agccataccc tggattttta
     1501 ccgtggcaat accatacagg ctttccataa aataggagct ggcccgggcc cccctgacaa
     1561 gagtttcttc cgatatttgc cggtaatagc cgtatgtcac cagacgaata agaacgtaaa
     1621 ccatggtaaa accgagcact atccaggtaa gatatcctcc atataacagc atcatcacaa
     1681 aaacacccac aaccataata ctgtccatga ttgccccaac aacacaggta gtaaaggtgg
     1741 ccctcaaagt gtcaagggag ccaaaacgcg actggatatc acctaattta cggcgttcaa
     1801 aaaacgccag cggcagtctg agaagatggt taaacagacc cgactgccac tgtatattga
     1861 tgagcgtgct cataaccagt gaggaccatg cacgcagcat actcaccgcg gccctgagca
     1921 atataaagaa catcaggcca gcagaaataa gcgtcagcag ccctctgtcc cccgccggaa
     1981 tcgcatgatc cataaccagc tgagttccca ccggcattac cagattgatt gcttcaatta
     2041 caactgacag acagaaaatt ttcgccagtg ttcttttaat accgtaaata ctgttaatca
     2101 gtgaacggag acttatgcgg gtctgctggg tttccgccag gaattcactt ccgggccaga
     2161 cctcaagtgc aatgcccgta aaataccggc tcatttcctc ccgaccgaga tatcttctgc
     2221 ctctggccgg atcatgcagt acataacggt tacgctttac gctgaccagc acgacgaagt
     2281 gactgaaatc ccagtggaga atacacggca ttttgagggc accaagttca tccagctcca
     2341 gtgaaagagc ccgggtgacc atccccagct gctccgctat tccattgatt cctgcaaggt
     2401 ttgctccacg ggccgagaga ttaaacttcc ggcgaagaga tatcaggtca atattcttac
     2461 caaaatgacc gcatatcatt gccaggcagg ccagtccaca ttcagcggtc tccgtctgat
     2521 gaataaccgg aacacgacgt cgccagcgca tatcaagctg atttataatt tgtctgaaat
     2581 tcccgttagt cattgagcgg tcctgttgca ctgtgtttca tgtcatagaa aggagaaagc
     2641 atccactggt aaatacgcct tttttccaga aatagtgtac tttcggcttt cattccattt
     2701 tccagaggga ggtatttttc gtcatatctg ataatctgct tttcaggcat cgcaatgact
     2761 ttataccagg gaacagagtc gcccggcgta ttctgtggtg ctcccttata ggtcaacatt
     2821 tcctgtgttg acgcaggagt cctggatata gttttaaccg tagcagagaa ctgcccaaat
     2881 ttttctgccg gaaaggcctc ataacgaata ttcactttgt caccagccga aatataagga
     2941 acagcatcat tcgggaccca gagaataaga taatagtttt caatgttctc aggaataacc
     3001 tgcagaaggc tgtctccggt attgaccatt tgcccgacag tgacactcag ggagtcaact
     3061 ttcccgtcag tcaacgcccg gataatgatt tcgccctcca catcagtgtt aaccagttct
     3121 ttctgtaact cgtaccgttg cagttccatc tggtagatac ggttatcaaa atctgcagcc
     3181 tgagtctgaa tctgactctc cagagtggtt atctgcaggg cattctgttc gttctgtccg
     3241 ctcaggctga gaagattgtt ttgttgctga taatataatg ccacctggtt agttaactga
     3301 tctttattaa tcagcccttt tgcctgatag tttctgtaat tctccatatt gtttttcatt
     3361 atttttatcc cttcctctgc acgctgtata atatctgatg agcgacgaaa cgcatctgtg
     3421 tattgcaggc gttgtttttc cagggtatcc agcgttattt ttttactttc ttccagacgg
     3481 gaaataatgt tgtccacacg aaccagctgg ttttctatat cccgacgatg attatcagtg
     3541 acaataccac tacgtgtact tttactgatg tcaatcagat aaacaggatc cccttttttt
     3601 atcaactgtc cttcatgaac aaactgcctg acaacaaatc cctgtacacc tgaatatata
     3661 ttgacagctc ttggccaggt tgtgacctca ccactgacat taacacggcg gctataggta
     3721 ccaacaataa tgaacatcag aaatgccgta ataaacacta tgcttcccag catgattaac
     3781 catagtggta ttccgggaag taatattgcc cgtccctgcc acttcatttt tctgttttct
     3841 aaagcatcct gacgaaacaa aatagctcct cttactcaca cttttcctgt aataccttca
     3901 ctggttaagc gaaatgctat aatcaaagcc agaaaataca gagtcctgtc aaaatagtat
     3961 cagcgatgca tttaccccgt aatttacaat tatctctgta tcttcagagg tactacgttt
     4021 tatgcagccg ctcagggatg tatgtcacaa ttttctttcg cctgcttaac catcgtactt
     4081 ctccttctcc ccatggctga agctgtataa gatagtcaat aacttttcga ccaaagcctg
     4141 gtttacaaca gaaatccaga atccaaatcc ttccatcttc attccattca gacggatgca
     4201 acctgaattc tggatccctg agcaggcgcg cttctgtatc agcctcaaga tatgcccagg
     4261 taatatacgc cagtggaaaa cctcgtgaat caaacaggaa tttaatctga ttatgctgta
     4321 tcgcatattc agtccagtat tgaatggtta acaccggata cagactgtaa ttacgattgt
     4381 agagcataca actgaccgca aaaccgatct gttcataaac agtccattca tcttttcctc
     4441 tgatggtttc ccttatataa gacatgatta ctatttccct tatatatttt ttacactgtg
     4501 tttcttatcg gttcaagata tccaaatgca tctctggaaa acttaagcat aaaatttttc
     4561 atatcagaaa ttgcttcttg taaaaaaagg ggatcatcgc catgattatc cagcaggatg
     4621 gcaatttttt cgcacatatt ttctttcctc agctcttccc tgggttgcag ccttttcatg
     4681 gtatgatgtt caatttcttt caggagtaat gaggcattta tctgacgtct ggtactgcct
     4741 tgcgtcagcc agtggatggc ctgggaaaaa cactgttgtg caacggtgaa atgaacatca
     4801 ttcttgatgc tactattcag tgcctgcttt tgattatgcc tatgatggat tagcgtcaca
     4861 accaactttt gcaatggtga accatcctga ttgacaatat cttgggcatg tgatttctcg
     4921 cagtattgat tgagtaatgc aacaaaactg ttaaccagtt ctttcaatat atcaggtatc
     4981 tctttttgtg ttataaattc tccaggaata cccaacagtt ctagataaca gtaataacat
     5041 gcttcatgaa aagttttctg aaatatctcc tcaaccttca aacgggctgc aagagtgaag
     5101 tcagggtcaa acatatattt tccgatatac aagcagagat cagacagccc ttgcataagt
     5161 accatcggtt cttttgccag ttcagctaat cttagtttat acccaaaatc agctggggaa
     5221 tctcccggaa acagacaact gagtccgtta aaatccatta atctgccatc cattgcaata
     5281 ttagaaggag aagttgtcaa tctcaggaaa cgtgttcgac aaaatgccat ttgccaggcc
     5341 tcacgacgtg ccagttcaca taacccctca atgcaataaa tccggggatc agttcgtgct
     5401 tcagcatcaa tttcttccgg tggtacaggt agatatcctg gcagcttgtg gatcacagat
     5461 ctaacccgac aggcatcgtg aattaacaga ctggaatact ccggttttac ttggaagtaa
     5521 ggtgcccgtt caaaatgcgc cggacgaaca acaggctcac gtaccaacag ggctcttcgt
     5581 gatttcctag cggagcgctc aaatgccttt tcagtataga gatctgtaag taaaaccgcc
     5641 cgaacccgca cagcactata aggtaatatt tgagccagta cttctcccca caaagcctca
     5701 tatattgcat gaacagcgcc gagtgcacca ttagaatgac gttcgtcagt accttcgcca
     5761 accaacggat tacttcctat tcctttaacc tggtagttac catcaaaccc acaacgtgca
     5821 ccacctccat tacgctgaat accactgcca ccatagcgct ctgcatggaa cactttggca
     5881 taagctgtac tgccaggatc gataatataa ctgcaggcat tttgcagctc gtctggattt
     5941 ctgacatcct ctgcacacca gattaatgag gccttgatct gcctggcagt gaaaggcacc
     6001 aggttttcat tcagttcaga aagtgaacac tgatgactca tctggcagta ttctccgttt
     6061 caattttcat aaaagagtgt tcataaaaga gcaagtaagt gtcctgacag cttcttcctt
     6121 attacagtaa aactctatta attacacata aattaaaaaa ttatttatca acgactgtaa
     6181 atcatatctt catcagtaaa gtgttgaaca taaaaacaca cttataaatg gatcttctat
     6241 ctgttttaaa caaaaaggat atattttaca ggtactaccg aaaaagtgcc tgtaaaataa
     6301 ataaaaatat aattagctac cgccaccagc agaagaactg gcactaccac ttcccacggt
     6361 cgagccaatt gctgttgtca aaccggcaga tacggctcca actacaaccc caagtggacc
     6421 accaggtatt ccggcgagag ctccaacaat agctgcagca cctgcagcat tagctgaagt
     6481 cgctggcgca cctcccgccc cggaaatata tcttaactgt gattctgtta tttctcgcat
     6541 ataaactgca tataaactcc atcatattta acttccgttt ttccagcctg accagaccag
     6601 aatccttcga atcccatgaa tcagctcaat aaaaccaaga aatattataa agcttagaaa
     6661 aacgtcaaca taagaattcc cttcgccaac aatccgaagg gatgaaagtg aaaccaataa
     6721 gatagtaata ggtaaactga atatagccgt caattgggac agttttttat aactcattaa
     6781 tccctacctg ttgctttatt ttcattatga aaagttacca aatcttagca cctcagtcag
     6841 gagagtcagg tcatcgataa agaatattcc agtagagtgg cgctcatgat caaagataac
     6901 agcaaagttg atgacagcgc tgaccgactg tcatttacag aaaccctgtt tgtagcacat
     6961 tccatcttac ctgacctcac ccagacaaag acaaaagaga atgattgtta tttacttttc
     7021 aaaaaaaagt gtcatattgt ccatataaag tcaagaggta ttttacgtta tgtcactgat
     7081 gcaattgtta gtcaggatct catggttgtc gctcatgtat aacctgagac ttgaacgcac
     7141 actaatagca ctgacggtca tacatcggga aatgtgagga ggcataagaa tgaattgtga
     7201 taataatcac agaaatgaag aattcattgt tacctttgat aaaggcaaca agcaagacaa
     7261 ttcaagacga aaacacgata attttcctat agaggtagaa tcctccgtag aacaggagac
     7321 acactgtatc acaaataata agtcggcttc cggtatagta acacatgact atgatgccga
     7381 ttatatttgt ggttgtggtg aaattatgtg tcctggttgc ggtcatgacc tataacaaac
     7441 atttttgcaa actgatacct gatcaggtac aagcctgttg attattatgc tgaatatttc
     7501 atgttataaa accatcgata taaacattaa caagaaaaaa catatattgt ttatctgaac
     7561 atttatcgat ggaaaaccaa aggggaaagt atgtatctta cgaaaaagat tataataagt
     7621 atgatgttta tattaccatc tgct
//
LOCUS       DAZGXZ010000202         7570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000202 DAZGXZ010000000
VERSION     DAZGXZ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.202"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHKJF_004888"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHKJF_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP0952107.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTGRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHKJF_004889"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHKJF_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP0952108.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHKJF_004890"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHKJF_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP0952109.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLCTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHKJF_004891"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHKJF_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP0952110.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHKJF_004892"
     CDS             7014..7292
                     /locus_tag="ACHKJF_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952111.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..>7570)
                     /gene="gadE"
                     /locus_tag="ACHKJF_004893"
     CDS             complement(7293..>7570)
                     /gene="gadE"
                     /locus_tag="ACHKJF_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0952112.1"
                     /translation="PIYFVSRKESIKNLLEITYGKHLPHKNSQLCFSHNQFKIMQLIL
                     KNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSYF"
BASE COUNT         1947 a         1779 c         1826 g         2018 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact ggccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgcagtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct catcaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcca gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacacag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 tagccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgggtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagc gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcaac tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg
//
LOCUS       DAZGXZ010000203         7546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000203 DAZGXZ010000000
VERSION     DAZGXZ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.203"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..448
                     /locus_tag="ACHKJF_004894"
     CDS             122..448
                     /locus_tag="ACHKJF_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952113.1"
                     /translation="MQTITTHEQLLVDGKITERTATHIVTGAHGYETLCTSGYNIRYN
                     EKQELVESCEKIAEGELPVTCPTCFAVWQDVHKFTPGDFDTESGKGDFADTNLTEIMI
                     GKMSDT"
     gene            complement(753..1070)
                     /locus_tag="ACHKJF_004895"
     CDS             complement(753..1070)
                     /locus_tag="ACHKJF_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFP0952114.1"
                     /translation="MKQFSVYKNKSRNKQAYPYFIDIQAEMLAHLNTRLVMPLTPKDN
                     SNSQVKTLTPVININQVDYVVLTTMITTTDAKNLNSDNVVMDASYLRDQLVSAIDMMI
                     LGI"
     gene            complement(1070..1315)
                     /locus_tag="ACHKJF_004896"
     CDS             complement(1070..1315)
                     /locus_tag="ACHKJF_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP0952115.1"
                     /translation="MRTNVAVKKRSINVYLNAGLLEEAKDLDLNVSAISNKALEKAVR
                     ERKRERWIEENRAGIEALNRFVEDTGIFSDDENFGVI"
     gene            complement(1695..2759)
                     /locus_tag="ACHKJF_004897"
     CDS             complement(1695..2759)
                     /locus_tag="ACHKJF_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFP0952116.1"
                     /translation="MDKKTKTYPFGEIEFFDQLEEHAWDQIHDGMLPIMDLYLDPTVT
                     DIFINRYDNIYTSVNGKYVKTDSSFSSERALKAWIDQIATVLRQPFSTSGNYKYGDVI
                     EPVLDARFPDGSRLMCTDPVISPQGSTVSLRKVPKQILEEKDFINSGMLTRDILDYLI
                     SVIQKRKNFIVAGNTGSGKTSLLRLLARYIDTMERVITAEDTQELHIHRYFPLGIALE
                     AAHRKDVNADLPSLVHLTMRGNPDRVWVGEVRTAEAIAAYYMVLASGTIGNATTLHSV
                     TPMGAIRKMTWLMSSYLSIDFQIAQDLIMSEVDVIIQCRRTSKHGRRITDIVEIREGK
                     IVPIFKFDEEQEVHVRFDKK"
     gene            complement(2816..4681)
                     /locus_tag="ACHKJF_004898"
     CDS             complement(2816..4681)
                     /locus_tag="ACHKJF_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952117.1"
                     /translation="MQANDEFLRLVFENIWPWWTYAWVLVFSLLCVFTFSGHAEKSGD
                     KSTLRFIIEAARKKLLVAWILCTLLFFLFVYIIAWKHFGEDSHKASLYMELLYERIGD
                     KSFSFGDYCGIAFVLLFPYACQIINRRLIRPKLSSWLRRFRVSQTSDTMSDIRVEKGK
                     YSPKNFNPLDYYKDGHFFLGLDENDKAIYVTDDYLSKVNIKILGATQTGKGVIQGVIA
                     DQAILKNWGLWFWDQKPDKFIYSIMKQRCESEGREIYEVDLNGEGIGDYAPFKNGTKR
                     EILDRFFNAFSLETGGTDADYYKNNAQEVMYYIYTFWDRSLNDLQKLLSGSDDRIPEE
                     KQEWIAKNGANIRTQLNKWLELPCLSPADGQGLNVTELVKSGAVIYVKGSTKDKVVRA
                     VLKSMLREWSAAVIREQPKNHVLGIIDEARFVISSEVADSLATILSSNASLSIAYQER
                     DDLLNIPDEKPQIAQSIKKGTETNTNLTLVYRCNDETAEWVAKNSGTTAKSLTKLEEV
                     ETDGYGAETWKGRRMIGQEEDYYIPVNTVLAVEERVGIMQTMFELSRVLFTCWVPVQR
                     FYTLPARPQTEQDNVQDCIKNDGSISPSDEDYFNPDMISDEELAMINKTVSSDSK"
     gene            complement(4708..5400)
                     /locus_tag="ACHKJF_004899"
     CDS             complement(4708..5400)
                     /locus_tag="ACHKJF_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952118.1"
                     /translation="MIYRFKTDFMRQIAMLFSLGYTRWFGGEIPLNPHYKFVSLKARF
                     DELYAINATAQQRFRRREKGVANCKFLVWFDEKNATLLWVLLATEGDVYNEEKFYDGM
                     NKKTRISVSGYELKTVPHRGRKPSVSWAMKKETYEKWHADIKKSIRTRNDENIEKLWY
                     SLRRVAGFSELRRQVFKLQRYAMTEWKRSRRGEYPYEKIFVGWLGRFKQSETITDEEL
                     LLIAKSKKADIF"
     gene            complement(6720..6935)
                     /locus_tag="ACHKJF_004900"
     CDS             complement(6720..6935)
                     /locus_tag="ACHKJF_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952119.1"
                     /translation="MSDKIQEITQKIEKLQIKLSKIEEEKNKIQSKLVKLEEEKKSIQ
                     RKLILQEKREELEMLLDKESFDILFNK"
     gene            7016..7474
                     /locus_tag="ACHKJF_004901"
     CDS             7016..7474
                     /locus_tag="ACHKJF_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952120.1"
                     /translation="MFRLAKPEDYEFIPDINLWELSFDKRPVRGVRCEDPVLGAQQYN
                     DTQQSILLNRQRQKLRKQNFFSLENISWDGIFDWCLSKGTQEECYLIVKLYYAKNKDE
                     HYSTLSKLKKHEGFYHESDLYLWVSMFFMSGNKIASTDKLTLVEEKGGYI"
BASE COUNT         2320 a         1643 c         1372 g         2211 t
ORIGIN      
        1 tttgtgatat ttgtcatatg tttttttgag caatacgaaa taagatgctc tcccgtcatc
       61 tgacgtatat ttttacggtc gccccaacaa tcacataaac caacaattat gagagacaga
      121 catgcaaacg atcacaacgc atgaacagtt attagttgat ggaaaaatca cagaaagaac
      181 cgccacacat atagtgacgg gcgctcacgg gtatgaaacg ctttgcacaa gcggctataa
      241 catcaggtac aacgagaaac aagaactggt agaaagttgt gaaaagatag cggagggcga
      301 actacctgta acatgcccta cctgcttcgc agtctggcaa gacgtacata agttcacgcc
      361 aggcgacttt gacactgaat cggggaaagg agattttgct gatacaaatc tcacagaaat
      421 catgattgga aaaatgtcag acacataaaa aaaacaatca tcacataaaa aataatgata
      481 cgactacgaa acaattcaaa caacattgca gaactgctcc aaagtttgga atatgccgct
      541 ttagcggcat ggaaggcgcg aaaaatggtt tcatagagtc acagatacca ggtcgcgccg
      601 atacccctaa ccagggagtt tcatgtcgcc aggaaccgcg tcagcctcct gctaagcctg
      661 accgcacgga acctacagct attcaggtgg taggtggtcg tagaccgtcg atgttacagc
      721 gatatttttt actatttact accctgcaaa aattaaatac ccagaatcat catgtcaatt
      781 gcagaaacca gttgatctcg taaatatgaa gcatccataa cgacattatc agagttcaga
      841 ttttttgcat ccgttgtcgt tatcatagta gtcagaacca cataatctac ctggttgatg
      901 tttattactg gcgttaacgt ctttacctgc gagtttgaat tatcttttgg cgttaaaggc
      961 atcaccaacc gggtgttaag gtgtgcaagc atctctgcct gtatatcaat aaaatagggg
     1021 tacgcctgtt tatttcgcga tttgttcttg tagacagaaa attgtttcac taaattaccc
     1081 caaaattttc atcatctgaa aaaatcccgg tatcttccac aaaacggttt aatgcctcaa
     1141 tgccagcacg gttttcttct atccagcgtt cacgcttacg ctcccttacc gctttttcga
     1201 gagctttgtt agagatagca gaaacattca gatcaagatc ttttgcttct tcaagtaacc
     1261 ctgcatttaa gtatacgttt atactacgtt tttttactgc cacatttgtt ctcataacaa
     1321 gccccccaca taaattatgc atataaacat agcatatacc caaaaccccg gatctagaag
     1381 tgtggaatat gccgctttag cggcatggaa ggcgcgaaaa atggcttcat agagtcacag
     1441 atgccaggtc gcgccgatac ccctaaccag ggagtttcat gtcgccagga accgcgtcag
     1501 cctcctgcta agcctgacca cacggaacct acagctattc agatggtagg tggtcgtaga
     1561 ccgtcgatgt tacagcaata tttcacacaa acacaattat ataagcacat aagcatataa
     1621 gcatataagc atataagcat ataagcatat aagcatataa gcatataagc atataagcat
     1681 atgatgctaa atgttcattt tttgtcaaac ctgacatgta cttcttgctc ttcatcaaac
     1741 ttgaatatag gaacaatttt cccttcacgt atttcgacga tatctgtaat tcttcttcca
     1801 tgctttgatg ttctccggca ctgaataatc acgtcaacct ctgacataat taaatcctga
     1861 gctatctgaa aatcgatact gagatagctt gacataagcc aggtcatttt gcgtattgcc
     1921 cccatagggg taacggagtg cagagttgta gcattaccta tagttccact tgccagaacc
     1981 atgtaataag cagctatagc ctctgccgtt cttacctccc caacccatac acgatcggga
     2041 ttaccacgca tagtcaggtg aaccagagaa ggaagatccg cattcacatc tttacgatgt
     2101 gcagcttcca gagcaatccc taacggaaaa tacctgtgta tatgaagttc ttgagtgtct
     2161 tcggcggtga taacacgctc catcgtatca atatacctgg cgagcaagcg taaaagtgat
     2221 gttttgccag aaccagtatt accagcaaca ataaaatttt tccgtttttg tataacacta
     2281 atgagataat caagaatatc tctcgtcaac ataccggaat ttataaaatc tttttcttcc
     2341 agtatttgct taggtacttt acgaagactt accgtagaac cctgcggtga aatcaccggg
     2401 tctgtacaca tcaaccggga accatcggga aatcgggcat caagtacagg ctcaatgaca
     2461 tcaccatatt tatagtttcc tgacgttgaa aatggctgcc tcaacaccgt tgcgatttga
     2521 tcaatccagg ctttaagtgc acgttcactg gaaaaggaac tgtcggtctt tacatacttg
     2581 ccattgacag atgtatatat attgtcatac ctgtttataa atatatcggt tactgtcgga
     2641 tcaagataca aatccataat tggtagcata ccatcatgaa tctggtccca tgcatgttcc
     2701 tctaactggt caaaaaactc aatttcacca aaaggatatg tttttgtctt tttatccata
     2761 tgtacaccat attattaact aaaaaatctg gcatcagcta gatgccagat ttattttatt
     2821 tactgtctga cgatactgtt ttatttatca tcgctagctc ttcatcacta atcatgtcag
     2881 gattaaagta atcctcatca cttggagaaa ttgaaccatc attctttata caatcctgaa
     2941 cattatcctg ctccgtttga gggcgggccg gaagtgtata aaaacgttga acaggaaccc
     3001 agcatgtaaa taaaacccga ctgagttcaa acatagtctg cataatacca actcgttctt
     3061 caactgcaag aaccgtattt actggtatat agtaatcctc ttcctgtcca atcattcgtc
     3121 gtcccttcca tgtttccgcg ccataaccgt cagtttcgac ttcttcaagt tttgtcagac
     3181 tcttcgcagt tgtaccagaa tttttggcaa cccattcagc cgtctcgtca ttacaacgat
     3241 aaacaagtgt caaattggta tttgtttccg tccctttttt tattgactga gcaatctgcg
     3301 gtttttcatc cggtatattt aaaaggtcat ctctctcttg atatgcaata gacaaacttg
     3361 catttgagga taatatagtt gccagactat cagcaacctc tgacgagata acaaaacggg
     3421 cctcatcaat tatccctaac acatgatttt ttggttgttc acgaataaca gccgcagacc
     3481 actcacgcaa cattgatttt aaaacagccc tgacaacctt atcttttgtg ctacctttta
     3541 cataaataac cgcacctgac ttaaccagtt cagttacatt taatccctga ccatcagcgg
     3601 gagatagaca aggaagctct aaccatttat taagctgcgt tctaatatta gctccatttt
     3661 tagctatcca ttcctgtttt tcctccggta ttctatcatc actaccagac aacaattttt
     3721 gtaaatcgtt taacgaacgg tcccaaaaag tatatatata gtacataacc tcctgtgcat
     3781 tatttttgta atagtctgca tcagtcccgc cagtctcaag agaaaacgca ttaaaaaacc
     3841 tatccaaaat ttcacgtttt gtaccattct taaatggcgc ataatcacct ataccttcac
     3901 cattcaaatc aacttcatat atttctcgcc cctctgactc acaacgttgt ttcataatcg
     3961 agtaaataaa tttatctggt ttctgatccc agaaccacaa accccaattt ttcaatattg
     4021 cctggtctgc aataaccccc tgaataacac ctttacccgt ttgcgttgcg cctaaaattt
     4081 taatatttac tttagacaaa tagtcatctg taacgtagat tgctttatca ttttcatcta
     4141 atcctaaaaa gaaatgacca tccttataat aatctaaagg attgaagttt ttaggcgaat
     4201 atttaccttt ctcaacacga atatcactca tagtatccga ggtctgagac acacgaaaac
     4261 gacgaagcca tgatgatagt tttggtctta taagacggcg attaattatc tgacaggcat
     4321 agggaaaaag tagcacaaat gctataccac aataatcacc aaatgagaaa gatttgtcac
     4381 caatccgctc atacaataac tccatataaa gtgatgcttt atgtgaatct tccccaaagt
     4441 gtttccacgc tattatgtac acaaagagaa agaacaaaag agtacataaa atccatgcaa
     4501 caagtaattt cttcctggct gcttcaataa tgaaacgcag agtactctta tcaccacttt
     4561 tttctgcatg tcccgaaaat gtaaacacac ataacaaaga aaaaactaaa acccaggcat
     4621 atgtccacca gggccatatg ttttcaaata ccaagcgaag aaactcatca tttgcttgca
     4681 taagcacctc acgctatgtt gataaaacta aaagatatca gctttttttg acttcgctat
     4741 taacaaaagc tcttcatctg tgatggtttc actttgttta aatcgtccta accatccaac
     4801 aaaaatcttc tcataaggat attcacctct tctggagcgt ttccactctg tcattgcata
     4861 tctctgtaac ttaaatacct gtctacgtaa ctcactaaac cctgccaccc tcctgagtga
     4921 ataccataat ttttctatat tctcatcatt tcttgttctt atcgacttct ttatatccgc
     4981 atgccacttt tcataagttt ctttcttcat agcccatgaa acgctaggtt ttctacctcg
     5041 atgtggaact gtttttagtt cataacctga aacagaaata cgagtttttt tattcatacc
     5101 atcataaaat ttttcttcat tgtatacatc cccttctgtt gcaagtaaca cccataaaag
     5161 agttgcattt ttttcatcaa accagacaag aaatttgcag tttgctactc ctttttctct
     5221 tctcctgaaa cgttgttgtg ctgttgcatt gatagcatag agttcatcaa aacgtgcttt
     5281 tagtgacacg aatttatagt gagggtttag aggaatttcg ccaccaaacc accttgtata
     5341 tcccagcgaa aacagcatgg ctatctgacg cataaaatcg gttttaaaac ggtaaatcat
     5401 cgaaatcctc ataaaagcta tgtagtaacc gctacataag agaagtcaac atatgggaat
     5461 accttataaa gagattcctt gcgctgcgct tacaggttaa atattatact gatgtccggc
     5521 tcgatgcgag cctttcgggc gccggactat caagcatcgc ttacgtaaca aaacgtgcgt
     5581 tgcgagctgc caactctcga cgcatttttg ggaagtcgaa agacgaaaaa agccaaaagc
     5641 cttcgtctgc caaacgaagg tgagttatca ggactgccaa ccctgataac agttactgcc
     5701 gaagccgtat gcctgtaatt gggctggtac ggctacgaag ggcgttcggg ggtgaaactc
     5761 ccctgtccga cccaatccac gccaaagcgt gagactgggc gacacggaaa cgggaacgta
     5821 ggtgactacg tgatccctgt tgatcttgaa cgggtgaaaa tcccgaatat ggggtgcttg
     5881 agaaggtaca acatattccc gacaggtggg ctgctgaggg catcgtgagg tgtcagggcg
     5941 ttgaggggaa acctggaaac gtcggtactg tgaggggcaa cctttgcaga tcgctgtaaa
     6001 gctcaagcct gtaattgcgt ccgaagaggt cgcattcggg taagtgataa ccagccatga
     6061 cttaagtgtc tggctatggt gttgacggca aattatgagt aagggaggtg ctttggaatc
     6121 tcggccccct gaaagccaga aatggtaagg cgtagcctag tagtttgtct gagccagtgg
     6181 cgggttatcc cgacaagatc agctaacgtg ggaagcactc tggaaaattt cgggtggcac
     6241 cgaaattaca ttcctgagtg tgggagagtg gacgagtagg cgcaaaaatc tacagacgat
     6301 atagccgaag tctgtagatg aatctgaaaa tactggaggc aaccagtaca gataagtagc
     6361 tgaaaattca cggtagtaac atggcagagg aaacattcat gtttcctctt tttcgtcctc
     6421 tttctgttgg ttcagtaaga ggacttccca aaaaacgtag gtatattttg ttatgaaatt
     6481 gactaaaaaa tcatgtttga cacatgtaag acaacaacga ttttcagtat tgatgcagga
     6541 tcatttctgc aaaaaattct gcttacgttg ttgataaaac gctcacatgt cctgtctaac
     6601 gatagggcat tgtaatgaac aatcatcgcg tttttcgcga tcataactaa cggtaatagg
     6661 cactaccaat gacaaattgc cctctgataa tcagagggca attctctcaa aaatcatgct
     6721 catttattga ataatatatc aaaactttct ttgtctaaaa gcatttcaag ctcttctctt
     6781 ttctcctgta gaatcagctt gcgctgtatt gattttttct cttcttctaa cttaaccagc
     6841 tttgattgta tcttgttttt ttcctcttca attttgctca attttatctg caatttctca
     6901 attttttgtg tgatttcttg gatcttatcg ctcatattaa acacctttgt tgaaaaaatt
     6961 tttgttttac ggtaaaccag aactaccgaa cacacaacag acaaaggata gcgttatgtt
     7021 ccgtctggcg aaaccggaag attacgagtt cataccagat attaacctat gggaactgtc
     7081 atttgacaaa cgccctgttc gtggcgttcg ctgtgaagat cctgttcttg gtgcccagca
     7141 atacaacgat actcaacaaa gcattctttt aaacagacaa cgccagaaat taagaaaaca
     7201 gaactttttt agcttagaaa atatatcatg ggatggaata ttcgattggt gtttatcaaa
     7261 gggaacacag gaagagtgtt accttattgt caaactttat tatgcaaaaa ataaggatga
     7321 acattatagc actttaagca aactaaaaaa acacgaagga ttttaccatg aatcagattt
     7381 gtacttatgg gtcagtatgt tttttatgag tggtaacaaa atcgcatcga ctgataaact
     7441 gacactagtt gaagaaaaag gtgggtatat atgatggaga gaaaacaact taatttaaga
     7501 ataaatgatc aatttcttat tggagaaatt gacaacaaat tacata
//
LOCUS       DAZGXZ010000204         7508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000204 DAZGXZ010000000
VERSION     DAZGXZ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.204"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            302..4225
                     /locus_tag="ACHKJF_004902"
     CDS             302..4225
                     /locus_tag="ACHKJF_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0952121.1"
                     /translation="MNKIYSLKYSSVTGGLVAVSELTKKVTTASRKKLRLSSVVLLPA
                     GVLFATGAMASHMWIDNFYVRDYLDFAQNKGVFTAGNENISIIKKDGSVYKLPDVPFP
                     DFSPVANKGATTSIGGAYSVTATHNKTSHQAIATQSWGQTNYKYVDRNTSDDFAVTRL
                     NKYVVETHGIVSGADTSLTKAQALERYGVNFKGEKKLIAFRAGSGYLALQTNGKTFNY
                     KDISYTPELLNGSFVLVEDWTSGHIVTHNLFDEFGNRTTSGDSGSPFLLYDNIDKKWV
                     VLGTLFGEYYYPNGNVRSAVNRWNQSTVDALKKKYTQDVNVNGNEVSQTSLANNKDNV
                     IHGGGTLNIGSNLNLGNGGLVFDTSQKYTVKGNNVTYTGAGVDIGENTTVDWYLKGVT
                     NDNLHKIGAGTLDVKVTQGGNLKTGNGTVILSAEKSFNNIYMASGNGTVQLNADKALG
                     GGDYAGIFFTENGGTLDLNGHDQAFKYIAATDSGTTIINTNKDKTATLSVNNTDAYIY
                     HGNVTGNTKIVHSFEQKQQNERLILDGDINTTNDISIKNAHLVMQGHATDHAVFRDGG
                     FSCSYPAPLKWLCGTDYVSEIQNKEKVVNQKQNTNYKTNNQVSDLSQPDWERRTFSFG
                     TLHLENADFSVARNADVKGDIDAKNSTVTLGSDTAYIDLHSGKNITNEGFTFRKDVRS
                     GTSVGTTEDISSFTGSVTADNSTLTINNKFSGGFTAENKSKVSVKSRDVVLNTGATIS
                     ADSTLTLEKGSRLTANMWLMNSGTINVSENAELNIQGYSIADKFIPSQHELGNVKMTA
                     SNATLTAGNYAMFSGDITADDSTAVRLNLGSDTSTLSEFNPNPELTSLMFDKYNTSWT
                     GKISALKGDASMVNTVWRMTGDSGLNTLKTSKSLTVFSSDNNKFSTLTVNDLTTSDST
                     FVLRSDSTGSDKVVVKNKLEGKNNNLLVDYVANDGKHNSLNLELVRAPKGTAADVFNS
                     QTQSVGFSDVTPVIEQKDNGDKTTWTLKGFNAVANPQSAEKAVHFMSAGYKSFLAEVN
                     NLNKRMGDLRDINGEAGAWVRIMSGSGSAGGGFSDNYTHVQVGADKKHELDGLDLFTG
                     VTMTYTDSNAGSDTFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAEL
                     GTKDYSSHSWYAGAEVGYRYHVTDDAWIEPQAELVYGAVSGKQFSWKDQGMSLSMKDK
                     DFNPLIGRTGIDVGKSFSGKDWKVTARAGIGYQFDLLANGETVLRDASGEKRIKGEKD
                     GRMLMNVGLNAEIRDNLRFGLEFEKSVFGKYNVDNAVNANFRYSF"
     gene            complement(4407..5617)
                     /locus_tag="ACHKJF_004903"
     CDS             complement(join(4407..5326,5326..5617))
                     /locus_tag="ACHKJF_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0952122.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYHSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQTSAYFGEGGV
                     RPPLEKIMPLLDKLREQYGVGPVCSELHIAPSTYYHCQQWHHPDKRSARAQRDDWLKR
                     EIRRVYDENHQVYGVRKVWRQLLREGIRVARCTVARLMAVMRLAGVLRGKKVRTTVSR
                     KTVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSSM
                     ETTLVLDALEQALWARHPSDTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYDN
                     AMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAEKAH
                     YASIGNNDLAA"
     misc_feature    complement(5220..5336)
                     /locus_tag="ACHKJF_004903"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(6001..6486)
                     /locus_tag="ACHKJF_004904"
     CDS             complement(6001..6486)
                     /locus_tag="ACHKJF_004904"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0952123.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQALGATR
                     TFVVCRKGTQQVVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDASFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTESAKQFYIHHGFTPSKTQLQTLFLKLP
                     Q"
     gene            complement(6474..6758)
                     /locus_tag="ACHKJF_004905"
     CDS             complement(6474..6758)
                     /locus_tag="ACHKJF_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0952124.1"
                     /translation="MIYGGFMKSDVQLNLRARESQRILIDTAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADEPAIEKLLARKPQWDV"
     gene            complement(6833..6973)
                     /locus_tag="ACHKJF_004906"
     CDS             complement(6833..6973)
                     /locus_tag="ACHKJF_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952125.1"
                     /translation="MAESGYGLAGLGMGKVKSINQYRLTPGFGGFTPVSHVTTACRLP
                     CR"
BASE COUNT         2050 a         1688 c         1790 g         1980 t
ORIGIN      
        1 tggaatcgct tcctggacac acactaccgg cggggctgga atgtcaacgt atcccaggtg
       61 atcatttccc ataacttaaa acagcttaga tccttgttca ccagaaagtt tcatccattt
      121 tcttccaccc aggttgcgga agaaccactt ttcatccgtc gttgacatgt ggcgtttgta
      181 acatttttta ctgtaatgac aaaaatttaa caggtattat tactaagcta tttatcgcgg
      241 tacgtgataa gtggagttta aattttaaac ttttttgaat gcttgaaaga gatttataga
      301 tatgaacaaa atctattcac ttaaatacag ctccgttacc ggaggactgg ttgctgtttc
      361 tgaattaact aaaaaggtta ctactgcatc aaggaaaaaa ctcagacttt catcggttgt
      421 tcttttacct gcaggagtgt tgtttgcgac aggtgcaatg gcatctcata tgtggattga
      481 taacttttat gttcgggatt atcttgattt tgcccagaat aagggggtgt ttacagcagg
      541 taatgaaaat atttctataa taaaaaaaga tggttctgtt tataaactac ctgatgtacc
      601 atttcctgac ttttctcctg ttgccaataa aggagcaacg acatcaattg gcggggctta
      661 tagtgtaaca gcaactcata ataaaacgag tcatcaggct attgcaacgc agtcgtgggg
      721 acagacaaat tataaatatg tagacagaaa cacaagtgat gactttgctg taactcggct
      781 aaataaatat gttgtggaaa cccatggaat tgtttccggg gccgatacat cactgactaa
      841 agcacaggct cttgagcgat atggcgttaa ttttaaagga gagaaaaaat taatcgcatt
      901 cagggcaggc tcgggttatt tggctctaca gacaaacggg aaaaccttta attataagga
      961 tatatcatat actcctgaac tacttaatgg ctcattcgtt ctcgtggaag actggacatc
     1021 cgggcatata gtaactcata acctttttga cgaatttgga aaccgtacaa cctctggtga
     1081 cagtggttcc ccatttttac tttatgataa tatagataaa aaatgggttg ttcttgggac
     1141 attgtttgga gaatattatt accctaatgg taatgtgcgc tcagcagtaa accgatggaa
     1201 tcagagtaca gtagacgctt taaagaaaaa atatactcag gatgtaaatg taaacggaaa
     1261 tgaagtgtca caaacctctc ttgctaataa caaagataat gttattcatg gtggcggaac
     1321 actgaacatc ggaagtaatc tgaatctcgg aaacggcggt ttggtatttg atacgagcca
     1381 gaaatatacc gtcaaaggaa ataatgtaac gtatactggt gccggtgtcg acattggtga
     1441 aaatactaca gttgactggt atctgaaagg tgttacaaat gataaccttc ataaaattgg
     1501 tgccggtacg ctggatgtga aggttaccca gggcggaaat ctgaaaacag gtaacggtac
     1561 tgttatcctc agcgctgaaa aatctttcaa caatatctac atggccagtg gtaatggtac
     1621 cgttcagctg aatgctgata aggcactggg aggcggtgat tacgcaggca ttttctttac
     1681 tgaaaatggc ggaacccttg atctaaatgg tcatgatcag gcatttaagt atatagctgc
     1741 aacagattca gggacgacca tcatcaatac caataaagat aagacagcca ctttatctgt
     1801 aaataacaca gatgcctata tttatcatgg taatgtcacc ggtaatacta aaatcgttca
     1861 ctcgttcgaa cagaaacagc agaatgagcg cctgattctg gacggtgata tcaatacgac
     1921 aaatgatatc agcattaaaa atgcccactt agtgatgcag gggcatgcta ctgatcatgc
     1981 tgtattcagg gatggcggat tttcctgctc atatcccgct ccgttaaaat ggttgtgcgg
     2041 cacagattat gtgtcagaaa tccagaataa ggaaaaagta gttaatcaaa aacaaaatac
     2101 aaactacaaa accaataacc aggtatccga tttgtcccag cctgactggg aaaggagaac
     2161 gtttagcttc ggtacacttc atcttgaaaa tgcggatttt tctgttgccc gtaatgctga
     2221 tgtgaaaggt gatattgatg cgaaaaactc cacggtgacg cttggcagtg ataccgctta
     2281 tattgacctt cattcaggaa aaaatatcac caatgaagga tttaccttcc gtaaggatgt
     2341 tcgttcagga acatctgttg gtacaacaga ggatatcagt tcgtttaccg gaagtgttac
     2401 agctgataat tcaacgctca ctattaacaa taagttctcg ggggggttta ccgcagagaa
     2461 taagagtaag gtttctgtta aaagccggga tgtggttctt aacaccggcg ccacaatttc
     2521 agctgacagt actctgacac tggagaaagg ctcacgactg acggctaata tgtggctgat
     2581 gaattcaggc acaattaacg ttagtgaaaa tgcggagctg aatattcagg gatattcgat
     2641 tgctgataaa ttcattccgt cacagcatga gctcgggaat gtgaaaatga ccgcgtcaaa
     2701 tgccaccctg acggcaggta attatgctat gttcagcggt gatatcactg ccgatgacag
     2761 tactgcagtc agacttaacc tgggctcaga tacatcaaca ctctctgaat ttaatcctaa
     2821 tccggaactg accagcctga tgtttgataa atataataca tcatggaccg gcaaaatctc
     2881 tgccctgaaa ggtgatgcct caatggttaa taccgtatgg cgtatgaccg gagattccgg
     2941 acttaacacc ctcaaaacca gtaaatccct gacagtattc agcagtgata ataataaatt
     3001 ctccacgctg acagttaatg acctgacgac cagtgacagc acctttgtgc tgcgttctga
     3061 ctctacgggc tctgataagg tggtagtgaa aaacaaactt gagggtaaaa acaataatct
     3121 tcttgttgat tatgttgcga atgatggaaa acataattca ctgaatcttg aactggtcag
     3181 agcaccgaaa ggaactgctg ccgatgtctt taattcacag acacaaagtg tgggtttcag
     3241 tgatgtgaca ccggtgattg agcagaagga taacggagat aaaaccacat ggaccctgaa
     3301 ggggttcaat gccgtggcta atccgcagtc agcggagaaa gccgtgcact tcatgtcagc
     3361 cggctataaa agcttcctgg cggaggtcaa caacctgaac aaacgtatgg gtgacctgcg
     3421 tgacatcaac ggcgaggccg gtgcatgggt ccgtatcatg agcggaagcg gctctgccgg
     3481 cggtggcttc agtgacaact acacgcacgt tcaggtgggt gcggataaaa aacatgaact
     3541 ggacggtctg gacctcttca ccggggtgac catgacatat accgacagca acgccggcag
     3601 cgacaccttc agtggtaaaa caaagtcagt gggtgcgggt ctgtatgctt cagccatgtt
     3661 tgattccggg gcctatatcg acctgattgg taagtatgtt caccatgata atgagtatac
     3721 ggcgaccttt gccgagctcg gcacaaaaga ctacagctct cattcctggt atgccggtgc
     3781 tgaagtgggt taccgctatc atgtcaccga tgatgcctgg attgagccgc aggctgaact
     3841 ggtttacggt gctgtttccg gtaaacaatt ctcctggaag gaccagggca tgagcctttc
     3901 catgaaggac aaggacttca acccgctgat tggccgtacc ggtattgatg tcggtaaatc
     3961 cttctcaggt aaggactgga aagtcactgc ccgcgcaggt atcggctatc agtttgacct
     4021 gctggctaac ggtgaaaccg tactgcgtga tgcgtccggc gaaaaacgca tcaaaggtga
     4081 aaaagacggt cgtatgctca tgaatgtcgg tctgaatgcc gagattcgtg ataatctccg
     4141 tttcggtctg gagtttgaaa aatcggtatt tggtaagtac aacgtggata acgcggtgaa
     4201 cgccaacttc cgttactctt tctgatagta aaagccaccg cttgtggact gaccccataa
     4261 agtttgacag ttcatgttaa gcggctttca gggcctggtt ttggtattct accggactca
     4321 ggccttttaa tttcaggatg atctgctcgt tggtaagcgt acagtgaatc gccccggttt
     4381 tcctggagag tgttttatct gtgaactcag gctgccagat cattgtttcc gatggaagca
     4441 taatgagctt tttctgcttc tgccggagga gtatggccca gccttcccag caatcgtcga
     4501 ttgttatacc agtccaccca cgtgagtgtg gccagttcca cttctgcacg gtttttccag
     4561 ctcttacggt gtattacctc cgctttgtaa agaccattga tgctctcagc catcgcgttg
     4621 tcatacgagt cgcctgtgct ccctgttgat gccagtaatc cggcttcctt aagccgctgc
     4681 gtgtaggcca gcgatacata ctgagagcct ttatcgctgt gatggatggt gtcagacgga
     4741 tggcgggccc acaacgcctg ctcaagtgca tccagcacca atgtcgtttc catggacgat
     4801 gagacccgcc atcccacgat atatccggca aacacatcaa tgatgaacgc cacataaacg
     4861 aagccctgcc atgtactgac gtaagtaaaa tcagccaccc acaactggtc aggacgttct
     4921 gccacgaact gacggtttac gcggtcacct gcggcaacgg ttttccggct gacggtagtg
     4981 cggacctttt taccccggag aacaccggca agtctcataa ccgccatgag gcgcgccact
     5041 gtacatctgg ccaccctgat tccttcccgt aacaactgac gccagacttt acgcacaccg
     5101 tacacctgat gattttcatc gtatacgcgc cgtatctctc tcttcagcca gtcatcgcgc
     5161 tgcgcacggg cactgcgttt atcaggatga tgccactgct gacaatggta atacgttgac
     5221 ggggcaatat gcagttcact gcataccggt ccgaccccgt actgctcacg cagcttatcc
     5281 agcagtggca ttattttttc cagaggcggt cgaactccgc cttcgcaaaa taagcggaag
     5341 tctggcgaag gatatcgtta ctgcggcgca gttcacgatt ttcacgttcc agctctttca
     5401 gacgctgacg ttcagcggtg gtgagtccac catcaccgcc cccggtatcc cgctcatgct
     5461 ggcgaaccca gacacgcaga gtctctggcg tacaaccaat ctttggagca atggaacaaa
     5521 ttgccgccca ttgtgagtga tattcgccct gactttccag aaccatacga actgcccgtt
     5581 gacggacttc gggggaaaaa cgagtatttt tagtcatcct gtttacctct ttctcaggaa
     5641 gtttagtctc caggatttcc ggggcggttc atgcactgaa aaaacgggat atttatgtca
     5701 atcccagctg gcgttatgca gatccccggg caggactcat cgaaggtaag gaataggaag
     5761 ctctgcgttc aataatctgc cgctctcttg gattaccatc aatacctggt gccactctgt
     5821 cagcaatcgc aacagaactg gattcaacat atcgtgatgt actgaaccga ctaccggaaa
     5881 atccggctgt cagatttgcg gaaaatggcg ataaaactga gttgatgtca gcgccagtga
     5941 tataagacgg taatggggat gaagactatt tcctggactg acttattatg tcagcctccc
     6001 ttattgaggg agtttaagaa acaatgtctg cagctgagtt tttgatggtg taaagccatg
     6061 atggatataa aactgttttg cgctttccgt aagtgcatga accataattg cacgtacacc
     6121 gatattttcg gccactctgt aacaacgacg aactgcatca tgtagtaaat ccgcaccaag
     6181 ccctttacca cggaatgacg catcaacagc cagtcgtgca agtataatga cagggatggg
     6241 gtcaggcata ttgcgccgaa gattgcctgt agcttctgta tggttgacac tgccagtagc
     6301 cagtgagtaa aaaccgacta cttgttgcgt acctttcctg catacaacaa aagtccgggt
     6361 ggctccgaga gcctgatttt ttagcccctt ctgctttaac cagtcatcaa gaacagcttc
     6421 accgctgaca aattcagcga cctgatgaaa tgaggataaa ggttctggtg ctgttacaca
     6481 tcccattgag gtttccttgc cagtaatttt tcgatggcag gctcatctgc aaccggagca
     6541 tcaagcattt cgatgaactc ttcatattgc ctgtcattaa aattaaacac ccgccggtca
     6601 agaataacat cttccgcagc tttacaggcc atttcaagaa tgaagtctgt acgtgactta
     6661 tgaagaattt ctgcggcggt atcaatgagt atccgctgtg attctctagc tctgaggttg
     6721 agctgaacat ctgatttcat aaagcctccg tatatcattt gctttacaac aatagcacag
     6781 tgacgatata tagcaaatga tatacacaaa gtaacagatt actgatgtcg cgtcagcggc
     6841 atggaaggcg gcacgctgtg gttacatgtg ataccggagt aaaaccgccg aagcccggcg
     6901 tcagccggta ctgattgata gatttcacct tacccatccc cagccctgcc agaccatacc
     6961 cgctttcagc catgccagag cctctgtgcg cggtcggagt ggtcccgaca agggtttacc
     7021 cgcagtcggg gcgtatctcc gcgttagcgg gccgtcaggg ccgcttacga gcgtgtattc
     7081 agtacgtcca gccagaagac tggcagcgat gataatgtag ttacaacatt cataattaaa
     7141 agcgactctg ttccggccct ttgggccggg gcggggccgc ttttcagtta tgagggaggg
     7201 gctttgtggt ttcggttctg cgctggaccg gggttttttc ggggtctgtt tttgcatgtt
     7261 gtaactaaac cggctccggt cggggcccgc cgcttgcggt gggaggtgca tatctgtctg
     7321 tccacaggac aggcagtgaa caggttttct ttttaaatga atgtaattaa gtagtttaaa
     7381 ggagatataa acaggtgttt aaaagataca ttgcaccctg taacgctgac ggctggcgct
     7441 ttatgacatg aacggttgta accttatggg gaagtccctt gcagttaaat gtggataagc
     7501 aaaattcc
//
LOCUS       DAZGXZ010000205         7471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000205 DAZGXZ010000000
VERSION     DAZGXZ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.205"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..557
                     /locus_tag="ACHKJF_004907"
                     /pseudo
     CDS             30..557
                     /locus_tag="ACHKJF_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            554..1540
                     /gene="ycdU"
                     /locus_tag="ACHKJF_004908"
     CDS             554..1540
                     /gene="ycdU"
                     /locus_tag="ACHKJF_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFP0952126.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(1733..1820)
                     /locus_tag="ACHKJF_004909"
     tRNA            complement(1733..1820)
                     /locus_tag="ACHKJF_004909"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1784..1786),aa:Ser,seq:gga)
     gene            2054..2992
                     /gene="ghrA"
                     /locus_tag="ACHKJF_004910"
     CDS             2054..2992
                     /gene="ghrA"
                     /locus_tag="ACHKJF_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP0952127.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3047..3784
                     /gene="ycdX"
                     /locus_tag="ACHKJF_004911"
     CDS             3047..3784
                     /gene="ycdX"
                     /locus_tag="ACHKJF_004911"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP0952128.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3808..4362
                     /gene="ycdY"
                     /locus_tag="ACHKJF_004912"
     CDS             3808..4362
                     /gene="ycdY"
                     /locus_tag="ACHKJF_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP0952129.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            4464..4955
                     /gene="ycdZ"
                     /locus_tag="ACHKJF_004913"
     CDS             4464..4955
                     /gene="ycdZ"
                     /locus_tag="ACHKJF_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0952130.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(5019..5852)
                     /gene="csgG"
                     /locus_tag="ACHKJF_004914"
     CDS             complement(5019..5852)
                     /gene="csgG"
                     /locus_tag="ACHKJF_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP0952131.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5879..6295)
                     /gene="csgF"
                     /locus_tag="ACHKJF_004915"
     CDS             complement(5879..6295)
                     /gene="csgF"
                     /locus_tag="ACHKJF_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP0952132.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(6320..6709)
                     /gene="csgE"
                     /locus_tag="ACHKJF_004916"
     CDS             complement(6320..6709)
                     /gene="csgE"
                     /locus_tag="ACHKJF_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP0952133.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6714..7364)
                     /gene="csgD"
                     /locus_tag="ACHKJF_004917"
     CDS             complement(6714..7364)
                     /gene="csgD"
                     /locus_tag="ACHKJF_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP0952134.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         1853 a         1661 c         1844 g         2113 t
ORIGIN      
        1 gcgtgtccac tattgctggg taagatcagg cggttaaagg catcaaccgc gaacaactgc
       61 agacgatgat tacggaatca ggcattgcgc tggatattag ctgtgataac acgccgcgcc
      121 agcaggttat tgggggaacg caggctgcgt tgaatgaatt tgccacgctg ctgatggccg
      181 ctggatacga accggttaaa ctcggtgtga gcggtgcctg gcacacgcgt ttgatggaag
      241 acggcgttca ggcaatgcgc gattatcttg ccggactgga tatcgcttcg ccagagcatc
      301 aggttctgat gaacgtaaca gcaaaaagcg aggtcgctcc gtcaattatc aaagaaaatc
      361 tctcattaca cttaactcat acggtgaaat ggactgaatc tttcgataca tttctgaata
      421 tgccaacccc ggtagctttc cttgaaataa gtaataagcc ctatttgggg aatatgttaa
      481 atgattttgc tggcgtggat cagcaacgag ttatgcattg tcgtaaagca tttagtgatg
      541 caaaggtatt caaatgattc ctgattattt aacttttatt cgctttcagg ataaacgaaa
      601 tctgatatac atttatgcta ttggacttat tctgataggc ttttattgga agaatgcagg
      661 gtttactttt ccatcagagg atattggtgt agttagtggg attctggccc tggtgctgta
      721 taattttatt tttgatctca aggcgtactg ggcttataaa tgcgtcacga agaatatcga
      781 tttttcgtgg tttaagaaaa agcagaacca caaaatagaa ttatttctta cacaacctct
      841 ggtggcagga tttctgtcgt taatcatgtt gagtgcaatg agttgggggc tataccagct
      901 tctaccctcg ttatatgcgc tgttcctgat ttcgttactt gggccgttgg tcatctttct
      961 gctgtttcgg atgatccgca ccagttatgt caagcaggtc gctatttcag tagcgaaaaa
     1021 agtaaaatat aaaagtctga ctcgctatgt gctgctttcg gtgtgcatct caacggttgt
     1081 taacctgctt actatcagcc cgttgcgtaa cagtgattct tttgtgacag aggggcagtg
     1141 gttaacgttt aaatcgataa ttgcattgct cattctttgt ggcgtagtgt tggcgattaa
     1201 tctgtttttt ctgcgcttct ccaagcggta cgcttttctg ggcaggcttt ttttgcagga
     1261 aatcgatctg tttttctcca gtgaaaatgc gttgtcgacc ttttttgcca agccgctttg
     1321 gcttcggtta ttcatattgc tggttattga aatgatgtgg attacgctgg tgtcggtatt
     1381 ggcaacgctt gtggaatggc ggatttggtt tgaagcctat tttttactct gctatgtacc
     1441 gtgcttaatt tactattttt tccattgtcg attcctctgg cataacgatt ttatgatggc
     1501 atgtgacatg tatttccgtt gggggcattt taataagtga ggaagttaca ggaagtgacc
     1561 agataacgca tatatgttct ctactatctt gcgcattttg attgttgact gagtaaccag
     1621 acagttgatg tgcacaattt cccctcgccc taacagacgt gggcgggggc acccggtgtg
     1681 tgcaatatac gaaaaaaaag cccgtacttt cgtacgggct cttctttaaa tatggcggtg
     1741 agggggggat tcgaaccccc gatacgttgc cgtatacaca ctttccaggc gtgctccttc
     1801 agccactcgg acacctcacc aaattgtcgt tcctgtcttg ctggaacggg cgctaattta
     1861 gggaaatcat gacctgaggt caacaaactt tttgaaaaaa tcgcgcgttt attcaaactt
     1921 caatcaatgt gtggttttag taagcgaaat ctgctttttt tgccaccgac cacggatttg
     1981 ttatgctggt ggcctttgta gatcataacg ataagtgcga ataaatttcg cacaacgctt
     2041 ttcgggagtc agtatggata tcatctttta tcacccaacg ttcgataccc aatggtggat
     2101 tgaggcactg cgcaaagcta ttcctcaggc aagagtcaga gcatggaaaa gcggagataa
     2161 tgactctgct gattatgctt tagtctggca tcctcctgtt gaaatgctgg cagggcgcga
     2221 tcttaaagcg gtgttcgcac tcggggccgg tgttgattct attttgagca agctacaggc
     2281 acaccctgaa atgctgaacc cttctgttcc actttttcgc ctggaagata ccggtatggg
     2341 cgagcaaatg caggaatatg ctgtcagtca ggtgctgcat tggtttcgac gttttgacga
     2401 ttatcgcatc cagcaaaata gttcgcattg gcaaccgctg cctgaatatc atcgggaaga
     2461 ttttaccatc ggcattttgg gcgcaggcgt actgggcagt aaagttgctc agagtctgca
     2521 aacctggcgc tttccgctgc gttgctggag tcgaacccgt aaatcgtggc ctggcgtgca
     2581 aagctttgcc ggacgggaag aactgtctgc atttctgagc caatgtcggg tattgattaa
     2641 tttgttaccg aatacccctg aaaccgtcgg cattattaat caacaattac tcgaaaaatt
     2701 accggatggc gcgtatctcc tcaacctggc gcgtggtgtt catgttgtgg aagatgacct
     2761 gctcgcggcg ctggatagcg gcaaagttaa aggcgcaatg ttggatgttt ttaatcgtga
     2821 acccttaccg cctgaaagtc cgctctggca acatccacgc gtgacgataa caccacatgt
     2881 cgccgcgatt acccgtcccg ctgaagctgt ggagtacatt tctcgcacca ttgcccagct
     2941 cgaaaaaggg gagagggtct gcgggcaagt cgaccgcgca cgcggctact aataaagcat
     3001 caggattcct gctatccttg gcgggaattg aatacaggag agagttatgt atcccgtcga
     3061 ccttcatatg cataccgttg ccagcacaca tgcatatagc acattgagtg attacattgc
     3121 ccaggccaaa caaaagggca ttaaactttt tgcgatcacc gatcatggcc cggatatgga
     3181 agatgctccg catcactggc acttcattaa catgcgtatc tggccgcgag tggttgatgg
     3241 ggtagggatc ctgcgcggca tcgaagctaa cattaaaaat gttgatggtg aaattgattg
     3301 cagcggtaaa atgtttgact cgctggatct gattattgcc ggttttcatg agccggtttt
     3361 tgcaccacat gacaaagcga ccaatacaca agcgatgatc gccactatcg ccagcggcaa
     3421 tgtgcatata ataagccatc ccggaaatcc caaatatgaa atagatgtga aagccgttgc
     3481 tgaagcagcc gcgaaacatc aggtggcgct ggaaatcaat aattcttcat ttttacactc
     3541 acgtaagggc agtgaagaca actgtcgtgc ggtagccgca gcggtacgtg atgctggagg
     3601 ttgggtggca ttaggctcgg attctcacac cgcgtttacc atgggggaat ttgaagagtg
     3661 tcttaaaatc ctcgacgcgg tagattttcc gccagagcgc attttgaatg tttcgccgcg
     3721 ccgcttactg aacttccttg aatctcgcgg tatggcaccg attgcggaat ttgcagacct
     3781 ttaattacta gtaatggaaa tatgttaatg aacgagtttt ctatcctctg tcgtgtgctg
     3841 ggttcgctct attaccgcca accgcaagat cctttactgg tgccgctgtt taccctgatt
     3901 cgtgagggga aactggctgc gaactggcca ctggagcagg atgagttact gacgcgttta
     3961 cagaaaagct gtgatatggc gcaagtctct gccgattaca atgcgttgtt tatcggcgat
     4021 gaatgtgccg tgccgccata tcgtagcgca tgggttgagg acgcaacgga agcggaagtg
     4081 cgcgcttttc tttccgaacg agggatgcca ctggcggata cgccagccga tcacatcggc
     4141 acattgctgc ttgcggcttc ctggctggaa gatcagtcaa cggaagatga gagcgaagca
     4201 ctggaaacac tgttcagtga gtatctgtta ccctggtgtg gtgcgttcct cggcaaagtg
     4261 gaggcccatg caaccacgcc tttctggcgc accatggcac cgctaacccg tgatgccatt
     4321 agtgcaatgt gggacgagct ggaagaagat tctgaagagt aaatgtgatc atcgtcactc
     4381 ataactgcaa cagataattt attgttgcat aaaatgtgtg ctcgatctca ttcatggccg
     4441 cgttttctgc tatcatgcgc ggcatgaaca tacttctctc tattgcaatc acaacaggca
     4501 ttctctccgg tatctgggga tgggtggctg tttccctggg cttactaagc tgggcgggct
     4561 tcctgggctg tacggcctac tttgcctgcc cgcaaggtgg gctgaaaggg ctggcgatct
     4621 ccgctgcaac cctgcttagt ggcgtggtgt gggcgatggt cattatttac ggtagtgcac
     4681 tggcaccaca tctggaaatt ctcggttatg tcataaccgg tattgtggcg tttctgatgt
     4741 gtattcaggc caaacagctg ctgctttcat ttgttccggg gacgtttata ggcgcatgcg
     4801 cgacgtttgc cgggcagggt gactggaaac tggtgttacc ttcgctggcg cttgggctgg
     4861 tatttggtta cgcaatgaaa aacagcggcc tgtggctggc ggcgcgtagt gcaaagacgg
     4921 ctcaccgtga gcaggaaatc aaaaataaag cgtgaggggc actcacgctt tcgcttaaac
     4981 agtaaaatgc cggatgataa ttccggcttt tttatctgtc aggattccgg tggaaccgac
     5041 atatggcggt atttcaccag aatgtcgttc tgccgttctg ctttgttctg caaatcccac
     5101 agaccacggt cgataccatc attaatcagg aaaatgaccc ctgtttcgat agccgacatc
     5161 agacacagca taacgggttc gtttgaggtg taacccactt ccccttccag caagcgctgg
     5221 tagtcaataa agcggaaaac cccggcctga acttcatagg aaagtatcgt cttactggtg
     5281 ttcaccgagg agaggatctc accggtactc acattgacga cgcgcaggtt cacggcaatc
     5341 tgatcgagct ggtattgcgt gtctgcaccg atcccaaaat atcttgcccc aacaccgccc
     5401 gatttaacgt tgctttcata accgataatc gaaccttcaa ccataatatt tgccgccgtt
     5461 aaagattgca gcgggattcg gttattaatg gcaaccgtgc cgttttcttg tgccgcacga
     5521 ataatcttgc gctcgttaag caggttttgt aagccctggc gctccagcgg tataaaccag
     5581 cgagaatctt tcaatgccgt gaccagcatt gccgtggcgc tttgcggaac agcagtggag
     5641 aagttacttg ccgggtaggg tttaaattgc ccggtttcgt cctgaatgtt gtataccgaa
     5701 acaaagatct tacccgtcgg cgctggcaga tgggtcaaat ctttgtagct ctgagctcga
     5761 ggcattaatg tcggtctggc ggcttcttta ggcggggcgg ttaagcatcc gctcagtaac
     5821 atgacggcaa ccaaaagaaa taagcgctgc atgattattt tccttatgaa gctggggctt
     5881 aaaaatcggt tgagttattt tgtaaacccg aaacctggat ggtcgaggtt tgtccggttt
     5941 tacgatctgt cacgttcaac tgcaattgac catcgcggtt agcaatatca acaatataat
     6001 cgttggtcac catgcggccc ggtttaccgg tattaatatt cgacagtagc ccacctaaaa
     6061 tttgtgactg gatggcctga gtaaagttat ctaacgctga gggtgtttca ataccaaagt
     6121 catcgttata gctcggatct ttataagagt tttgggcctg agcgctattt aataaaaaag
     6181 cgccattatt tgggttacca ccaaagtttg gattacggaa ctggaaagtc atggttccag
     6241 cccaacttaa tggcgaaata agcatgagta gaactactgc atgtttgaca cgcattgcag
     6301 cctccgaaca attttttatt tagaattcat catgcgccaa atcgcccgta cttaataacg
     6361 cctgatttat ctggcgacga tttagtgctt cttcagtttg aatcagtgca aagacgacag
     6421 ttttctcgaa gtctcttttc aacggaaata aaaaagtctg gaaaataacg tcctgattga
     6481 ccgttatagt gatccagctt ccccatcgtg cactgggcct ttcattaatc gttaagttac
     6541 ccgtatagtc actttcccat ttatcactaa aggctcggta aaaatcatgg ccaatagatg
     6601 aaacagtatg gtcagttaac aatcccggga cttctacctc aacggcgtga agattccctg
     6661 cggcgaacag aaattctgct gccacaatcc agcgtaaata acgtttcatg gctttatcgc
     6721 ctgaggttat cgtttgccca ggaaaccgct tgtgtccggt tttttacggc tatcttcttg
     6781 aaaagattat aaagatgcgt tttaaccgta ttttcgctga tgaacaacga acgagcgatc
     6841 tcgttattag acgcgccgat acgcagctta ttcaggatct ctttttcccg atgagtaagg
     6901 agggctgatt ccgtgctgtt ataacgatag ttaccagaat gtgtaatcag gtaactggca
     6961 agcttttgcg taaagtagca ttcgccgcgc aggacgcctt gcaacccatt gacaacacgt
     7021 tcttgatcct ccatggcata aaaaacgccg ttgatatgag gccagttttc aatgtcgcgg
     7081 tacgggtaat cttcaggcgt atttagcaac aatattttga tattgttgtt tttcctgctc
     7141 aaagtatcct gccaataatg gataagcttt ttatctgctt ccatcatatc cagaagaata
     7201 atagagcctg aagatatatc gtccagagaa cgttgaatat tatgtaattt tcctgtaatt
     7261 gccagcgatt gtttaaggtg ctgcaagaga gctgtcgctt gcaaagacgg tttagtgatc
     7321 aacaataatg tatgaccatg aatactatgg acttcattaa acatgatgaa accccgcttt
     7381 ttttattgat cgcacacctg acagctgcct ctaaaataga agcaccagaa gtactgacag
     7441 atgttgcact gctgtgtgta gtaataaatc a
//
LOCUS       DAZGXZ010000206         7391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000206 DAZGXZ010000000
VERSION     DAZGXZ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.206"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..576)
                     /gene="folK"
                     /locus_tag="ACHKJF_004918"
     CDS             complement(97..576)
                     /gene="folK"
                     /locus_tag="ACHKJF_004918"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP0952135.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(573..1970)
                     /gene="pcnB"
                     /locus_tag="ACHKJF_004919"
     CDS             complement(573..1970)
                     /gene="pcnB"
                     /locus_tag="ACHKJF_004919"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP0952136.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(2030..2926)
                     /gene="gluQRS"
                     /locus_tag="ACHKJF_004920"
     CDS             complement(2030..2926)
                     /gene="gluQRS"
                     /locus_tag="ACHKJF_004920"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP0952137.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2993..3448)
                     /gene="dksA"
                     /locus_tag="ACHKJF_004921"
     CDS             complement(2993..3448)
                     /gene="dksA"
                     /locus_tag="ACHKJF_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP0952138.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3626..4330)
                     /gene="sfsA"
                     /locus_tag="ACHKJF_004922"
     CDS             complement(3626..4330)
                     /gene="sfsA"
                     /locus_tag="ACHKJF_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP0952139.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4345..4875)
                     /gene="thpR"
                     /locus_tag="ACHKJF_004923"
     CDS             complement(4345..4875)
                     /gene="thpR"
                     /locus_tag="ACHKJF_004923"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP0952140.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4949..7378
                     /gene="hrpB"
                     /locus_tag="ACHKJF_004924"
     CDS             4949..7378
                     /gene="hrpB"
                     /locus_tag="ACHKJF_004924"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP0952141.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKDLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPRHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1696 a         1910 c         2057 g         1728 t
ORIGIN      
        1 ttatgtttct gcaaaaatgt ttaaactaaa atttttatta aggaaagaga ggatgtatga
       61 ttttcagaaa atatttatat tcgtaatata aataaattac catttgctta atttgtcaaa
      121 tgctcttgta tgtaagactt cacgcaacgt ctccccatca ggaaacgcca gctccggtgc
      181 aatctcaaac agcggccaca gcataaatcc acgattcttc atatcgtagt gcggaacggt
      241 caggcgttcg gtatttatca cttcattacc aaacagcatg atatcgagat ccagcgtgcg
      301 tggtccccag cgttcagctt tacgaacacg accctgttgc agttctatgc gctgtgtatg
      361 gttgagtaac tcttccgggg cgagcgaagt ttcaagcgcc acggcggcgt tgaggtaatc
      421 gggttgatct tgcggcccca gcggtggggt gcggtaaaac gaagaaacgg caagaatgtg
      481 gctttcaggg atatcgccta atgctttcag ggccgcattg acctgctcca gcggagaggc
      541 cagattgctg cctatggcaa tatacgccac tgtcatgcgg taccctcacg acgcggtgcg
      601 cgtttgcgtg gacgacgagt acggcgacgc ggtgacggct cttcatccaa ctcgttaagc
      661 atcccttttt gatctggtgg cgcggaaacc tggaactcac cccaccattt caccagacgc
      721 tgcagttcag cgttacgttc aacttcagct cgcaaggcca acaggtcata agccgcacgg
      781 aacttaggat gctccagcag tttccatgcg cgtttaccct gacgacggga catacgcaac
      841 tgcaactgcc agatatcgcg ggttaacgtc gtcagacgtt tcgggattgc cagtgaacgg
      901 caggcttcgt ccagcacgtc gttcatcgcc agcgcgaaag cgtcgtgata ggtcaggccg
      961 ctttcctggg caatcttctg tgccgtctcc agcagtgggt accagaacat ggcggcaaac
     1021 aggaacgccg ggttcacgcg catatcgtta tggatacgcg tatcggtatt cttcagcacc
     1081 tgttcaatga tccgctccat cgggctgtcg ccattttccg tgaagtagcg ggtaatggtc
     1141 gggaacagcg gctggaacag atgatattca cacaacagct tataggtatc gtaaccgtag
     1201 cccgcttgta gcagtttaag cgattcttca aacaggcgtg ccggtgggat atcgttcaac
     1261 agggtagcga ggcgagggat cggttctgcg gtttccgggc tgatgcgcat acccaatttg
     1321 gcggcaaaac gtaccgcgcg cagcatacgt accggatctt cacggtagcg cgtttccggg
     1381 ttaccaatca gacggataac gccgtccttc agatccttca taccgccaac gtaatcacgg
     1441 acggtaaaat ccgctacgct gtaatagagg ctgttgatag tgaaatcgcg gcgctgggcg
     1501 tcttcttcga tggagccgaa aatgttgtcg cgcagcaaca tgccgttttg cccgcgttgg
     1561 gaggtcgtgc ggtcgctgac gttaccttcg tggtgtccac ggaaggtcgc aacttcgata
     1621 atctccgggc caaacattac atgagccaga cggaaacggc gacccaccag gcggcagtta
     1681 cggaacagtt tgcgcacctg ctcaggcgtg gcgttagtgg ttacgtcaaa atctttcggc
     1741 tttttgccaa gtaacaggtc gcgcacgccg ccgccaacca gccaggcttc gtatcccgct
     1801 ttgttgagcc tgtacattac cttcagggca ttttcactga tatctttgcg ggaaatagca
     1861 tgctgctcac gcgggatcac cgtcacctgt ggacgggcga ctgcctgttc agcctcgctt
     1921 tcctcgcggc ttagcacctt gcggcaaaaa ttagcgactc gggtaaaaat agtacacctc
     1981 ggtagtgtca aacatcattc aggacaaaaa aatagcggct aatcatagct cagcatgacg
     2041 catttgagaa tgttgaattt acaattgccg actcgggcac ggcggtaagc cgccagtttt
     2101 tgacggctga ctgaaggatt tgctcgacgc tgaaatcctg ccagtgtgct tctgcctgct
     2161 gccccagaaa ttgaagtgcc gcgattagta ccgggcgtgg atcgcctttc ggcaacgcag
     2221 gcgcatgatt ctgcttggaa agtttagcgc cttgtggatt aagcgccagc ggcagatgaa
     2281 tgtaatctgg cactttccag ccaaaaagct ggtacagcga gatttgcctt actgttggtt
     2341 caatcagatc agccccacgc actatttctg taacgccctg gaaatgatca tcaaccacaa
     2401 cagccaggtt gtaggcgaac aacccatcac ggcgatgaat gataaaatct tcccgtgcca
     2461 gtttttcgtc ggcgtgaata atgccgcgca gctggtcagt aaattgcgtg accggatgct
     2521 gctggcggat acgcactgcg gcattgtctg gtccatgatg caacacccgg caatgaccgt
     2581 cgtaaatacc gccaatgctt tgaatacgcg cacgcgtaca ggtgcagtaa taacttagtc
     2641 cttgttcatg taaccaggcg agtgcttcac gataggcgtg gtgacgttgc gattgccaga
     2701 gaacatcgcc gtcccagtgc agaccgtaat gttccagctg gcgcaggata gtttctgcgg
     2761 caccgggaac ttcacgaggc gggtcgatat cttctatgcg taccagccag cgaccttgcc
     2821 gggcgcgagc ctgcaaatag ctgccgagcg cggcgatcag agagccaaaa tgaagctcgc
     2881 cggaaggaga gggggcgaag cggccaatat actgtgtgtc tgtcatctct ttgaacaaaa
     2941 aataaggcgg gagcatttcc cgcctgtggt aaacgtgatg gaacggctgt aattagccag
     3001 ccatctgttt ttcgcgaatt tcagccagcg ttttgcagtc gatgcacaga tcggctgtcg
     3061 ggcgcgcttc cagacggcga ataccaattt caacaccgca ggattcgcag tagccgaaat
     3121 cttcgtcttc cacttttttc agcgtcttct cgatcttttt gatcagctta cgctcgcgat
     3181 cgcggttacg cagttcgagg ctgaactctt cttcctgggc tgcacggtct accgggtccg
     3241 ggaagttggc tgcttcatcc tgcatatgtg taacggtgcg atcgacttca tccctgagtt
     3301 gattacgcca tgcttccaga atacgacgga agtgcgccag ctgggcttca ttcatatact
     3361 cttcgcccgg cttctcctga tatggttcca ccccagcgat ggcgagaata ctcagggacg
     3421 atgttttacg gttttgccct tcttgcatgt tgcttctcct taacacgcac tatcgatccc
     3481 catgttcggg ggaaaaatga ggccgctata aatagcagat gcttttccgg atagcaatta
     3541 tctaaacgta acacttgaca actgtgtgag gaaaagcgta tttgcgcacg cgaccagaat
     3601 gtaaattaac cagttactta ctttactaca atgtaaccgg cagtgatttt ttaagagcca
     3661 tgccttcagc agaaatttcc gctttgtaag ccagaatttc tacccctctc tgttgagctt
     3721 ctgacaatag ttgcgcgtat ttctcatcga tgtggcgcgc gggtgaaaac cgtgtaatgg
     3781 ctgaatgcag cacggcgaaa aagataaccg cacgctggcc ttcagccgct acgctcatca
     3841 actcccgaag gtgtttctga cctcgttcag tgaccgcatc gggaaaatat ccctgttcgt
     3901 tctccgctaa cgtaaccgat ttcacttcaa tatagcagtc tggacgcgaa tccgcctgca
     3961 acataaagtc aatacggctg cgttctgcgc cgtattttac ttcgcttttc agcgagctat
     4021 agcctgacag ttctgaaatt gattcattaa ggatagcctc tttcgtcaac ctgttagccc
     4081 aaagcgtgtt gacgcaaata aatgcgccgc tctggctttg agttaattcc caggtgtgtg
     4141 ggtatttccg tttggtgttg tctgaagtcg aataccagac ggtatcgcca ggcgttgcac
     4201 aaccggtcat tgcacccgta ttcgggcagt gtagcgttaa ttcgcgacca tcgggtgtga
     4261 tcacatcggc taaaaaacgt ttgtaacgct gaattagcgt cgcgcgctgt agagggggag
     4321 aaaattccat tgcgacaatc cttgttattg cgttagcgcc cagcgtttta gcggcgtgta
     4381 gcgtgtgcgt ccacgggcaa acgaggaggc gtaaagggtg aactccgtca ccgcataaga
     4441 ccagttaaaa cctggcggcg ggattgtcac cgcctcgctg gcgtcgcgca ataaggtaat
     4501 atgtggatga aacggacgat tgctttgaaa acaaccgctg cgggcagcct gtgaacggag
     4561 catattcgcc agctggatta agccacgtgg aggctgacgc atccctaacc acaccacacg
     4621 cgaacgcagc cattgtccgg cgtcatcaag cgtgagtgtg aaaccaggtt gacgaatccg
     4681 tccggctaaa agagaaagcg ccttctcttt ctcggcgctc acttcgccta aaaatgccag
     4741 agtcagatgc aaattatcgg cggcgaccgg acgtcccgcc tcaggtggga agtgtgtggc
     4801 gcgccaatgg ataatctgtt cgcggatttc tgcaggtaag tcgatagcaa agaacagacg
     4861 ttgcggttca gacatgtgag gcactcggtt atgaattacc gcgatgctac aatgtggcgc
     4921 gaagaatgtt aaccctctgg agcgttttgt gtcgtcgttg cccgttgctg ccgtcttatc
     4981 tgaattactt accgctctcg attgtgcgcc gcaggtatta ttaagtgcgc cgaccggggc
     5041 cgggaaatca acctggctgc cgctgcaact gctggcgcat cccggcatta acgggaaaat
     5101 tatcctgctg gagccgcgtc gtctggcggc gcgtaacgtc gcgcaacggc tggcggagct
     5161 gcttaacgaa aagccaggcg ataccgttgg ctaccggatg cgtgcgcaaa actgcgtcgg
     5221 gccgaatacc cgcctggaag tggttaccga aggcgtgctg acgcgcatga tccagcgtga
     5281 cccggaactg agcggtgttg gactggtgat ccttgatgaa tttcatgagc gcagcttgca
     5341 ggcggatttg gcgttggcgc tgttactcga tgtgcaacaa ggtctgcgtg atgaccttaa
     5401 actgctgatt atgtcggcta ccctggacaa cgaccgcttg cagcaaatgc tgccagaagc
     5461 gcctgtcgtc atctcagaag ggcgctcgtt tccggttgaa cgccgttatt taccgctgcc
     5521 cgcgcatcag cgttttgacg atgccgttgc ggtagccacc gctgaaatgc tgcgtcagga
     5581 aagcggatca ttactgttat ttttacctgg cgtcggagaa attcagcgtg tgcaggaaca
     5641 actggcttcg cgcatcggca gtgatgtatt gctctgcccg ctgtatggcg cgttgtcgct
     5701 gaacgatcag cgaaaagcga tcctcccggc accgcaaggg atgcgcaaag tggtgctggc
     5761 gaccaatatt gctgaaacca gtttaaccat cgaaggtatt cgtctggtgg tggattgtgc
     5821 ccaggaaagg gtggcgcgtt ttgatccacg cacggggctt acgcgactga ttactcaacg
     5881 cgttagccag gcatccatga cgcagcgtgc cgggcgcgcc gggcgtctgg agccgggtat
     5941 ctgcctgcat ttaatcgcca aagaacaagc agaacgcgct gccgcgcaaa gtgaaccgga
     6001 gatcttacaa agcgatcttt ccggtttgct gatggaatta ctgcaatggg gatgcagcga
     6061 tccggcgcag atgagctggc tggatcaacc gccagtagtg aatctactgg ccgcgaaacg
     6121 tctgttacaa atgctggggg cgctggacgg tgaacggctt agtgcgcaag ggcaaaaaat
     6181 ggcagcgctg ggtaacgatc cgcgtttagc ggcaatgctg gttagcgcga agaacgacga
     6241 cgaagctgct accgcggcaa aaattgccgc cattctcgaa gagccgccac ggatgggcaa
     6301 tagtgacctg ggcgtggcgt tttcgcgcaa tcaaccagcc tggcagcaac gtagtcagca
     6361 actgttaaaa cgcttaaacg tacgtggcgg tgaggcagac agttcgctta tcgcgccgct
     6421 acttgccggg gcgtttgccg atcgcattgc tcgtcgccgt gggcaagatg gacgctatca
     6481 actggcaaac ggcatgggag cgatgctcga tgccaacgac gcgctaagcc gccacgaatg
     6541 gttgatcgca ccgttattat tgcagggcag cgcctcgccg gatgcgcgga ttttactggc
     6601 gctgccggtc gatattgatg agttagtaca acgctgcccg cagctggtac agcagtctga
     6661 cactgtggag tgggatgacg cgcaaggtac gctgaaagcc tggcgtcggc tgcaaatcgg
     6721 tctgctgacg gtgaaagtgc agccgctggc gaaaccctcg gaagacgagt tgcatcaggc
     6781 gatgcttaat ggcatccgcg ataaagattt aagcgtgctc aactggacgg cggaagcgga
     6841 acagctacgc ttgcgtttgt tatgcgccgc aaagtggttg ccggaatatg actggccagc
     6901 ggttgatgat gaaagtttat tggcgacgct ggaaacgtgg ctgctgccac atatgactgg
     6961 cgtacattca ctacgcggcc tgaaatcact cgacatttat caggcactac gcggattact
     7021 tgattgggga atgcagcaac gtctggatag tgaattgcct gcgcattaca ctgtgccgac
     7081 gggaagccgg atcgccattc gttatcatga agataacccg cccgcgctgg cggtgagaat
     7141 gcaagagatg tttggcgagg ccaccaatcc gacgatcgcc caggggcgcg tgccgctggt
     7201 gctggagttg ctttcacctg cccaaaggcc attacaaatc acacgagatt tgagcgcctt
     7261 ctggaaagga gcgtaccgtg aggtgcaaaa agagatgaaa gggcgttatc ccagacatgt
     7321 ctggccggac gacccggcaa atactgcacc gacgcgacgg acgaaaaagt attcgtaagg
     7381 ttggttttct c
//
LOCUS       DAZGXZ010000207         7326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000207 DAZGXZ010000000
VERSION     DAZGXZ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.207"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(42..866)
                     /locus_tag="ACHKJF_004925"
     CDS             complement(42..866)
                     /locus_tag="ACHKJF_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP0952142.1"
                     /translation="MDKPKAYCRLFLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(920..2638)
                     /gene="proS"
                     /locus_tag="ACHKJF_004926"
     CDS             complement(920..2638)
                     /gene="proS"
                     /locus_tag="ACHKJF_004926"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP0952143.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2750..3457)
                     /gene="tsaA"
                     /locus_tag="ACHKJF_004927"
     CDS             complement(2750..3457)
                     /gene="tsaA"
                     /locus_tag="ACHKJF_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP0952144.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3454..3858)
                     /gene="rcsF"
                     /locus_tag="ACHKJF_004928"
     CDS             complement(3454..3858)
                     /gene="rcsF"
                     /locus_tag="ACHKJF_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP0952145.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3976..4791)
                     /gene="metQ"
                     /locus_tag="ACHKJF_004929"
     CDS             complement(3976..4791)
                     /gene="metQ"
                     /locus_tag="ACHKJF_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP0952146.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4831..5484)
                     /locus_tag="ACHKJF_004930"
     CDS             complement(4831..5484)
                     /locus_tag="ACHKJF_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP0952147.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5477..6508)
                     /gene="metN"
                     /locus_tag="ACHKJF_004931"
     CDS             complement(5477..6508)
                     /gene="metN"
                     /locus_tag="ACHKJF_004931"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP0952148.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6696..7268
                     /gene="gmhB"
                     /locus_tag="ACHKJF_004932"
     CDS             6696..7268
                     /gene="gmhB"
                     /locus_tag="ACHKJF_004932"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP0952149.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT         1802 a         1895 c         1855 g         1774 t
ORIGIN      
        1 ttcacgacgc atccggcatt ttgtgcacga ggctggcttt atcactgagt cagcccaaca
       61 aagcgacccg ccttgatata aatcccaggc ttcaggtgcc caacatactg taactgcgtt
      121 tctggcttaa acgcagagac atcaccaata cgcatatgca tcagatcggc agggctaatc
      181 cagcccgatt gcgccagtgt aagcggatgc ccggctttgg caaatgcatc agtgacaaat
      241 tccgaacaaa accacgactt tttgtctcct tcgcccacac tgcttaactg cgctttcgcc
      301 aggccgctga cgcactgttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg
      361 cgagtcacca taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg
      421 attttattgg caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt
      481 aagacgaaaa gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca
      541 gcgcctgtcg cttctgcaac gttattatca cccagataga ttgcaacgtg actcacagag
      601 gaagtgctga agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa
      661 tcgccgggtt tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt
      721 tgatgctgga atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg
      781 tctggctggc tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaaacagg
      841 cggcagtacg cctttggttt atccatttta tacaatccat gtaaaaaaag ggccctgaaa
      901 ttcaggaccc tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac
      961 cagtcttaat taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt
     1021 cgaggttacg gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa
     1081 acatcacgcc cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca
     1141 gttcgctgta cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg
     1201 gcagaatcgc cacctggaac ggcgcgatag cgtcaggcca tacgatgcct cgttcgtcgt
     1261 agttctgctc aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg
     1321 tcaggatttg gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact
     1381 tggtacccag ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct
     1441 ggccatccgg gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag
     1501 cgacatcgcg atcccagttg atgccgaagt agtgtttacc atcgatgtta gcaccagcag
     1561 cgaaatcact catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg
     1621 gacccagtga acccggaccg gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag
     1681 tcagcgggct tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac
     1741 cgcgcaccag cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag
     1801 tcttaaccgt tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg
     1861 cgttcggcgt atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg
     1921 cgatagcttc tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat
     1981 cgtcttcacc gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac
     2041 cggtgtcggc ttgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg
     2101 ccgcatacat tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag
     2161 catctttcat caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc
     2221 ggaacttggt ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt
     2281 tacgaatcag gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc
     2341 cacggtcaac aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc
     2401 acaaatcagc tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct
     2461 cttcacgcac gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat
     2521 ataacccgga ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca
     2581 cctcggcgtc ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt
     2641 tgttacggtt ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaaagt
     2701 gatttagttt accagtgcaa aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt
     2761 ccagcgcaaa gacttcaaaa cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta
     2821 gccagacggc ataggttttg cccgtttcct cacctttacg ataggccggg cgtgggtcct
     2881 gcgccagtac ttcgcggata aacagcgtta actgcggata acgcttctcc agcgtcaaaa
     2941 gctgcttttc gacctctgcg gtaaaactca ccgccatctc tgccgctggc gcactttgcg
     3001 catagctggc actggcatcg ggaagcgatt cggcaaaggg gagatacggt ttgatatcca
     3061 ctaccggcgt accatcgacc agatccagac tgccgagctt cagaatcacg ctgtctttat
     3121 ggcaaacaac ctctttcagc tctaccagcg acataccaat tgggttaggg cggaaagtag
     3181 agcgtgttgc gaaaaccccc attctggcgt taccgccgag gcgcggtgga cgcacagtcg
     3241 gacgccagcc gccttccatc gtttgatgaa agacgaaaag gatccataaa tggctgaacg
     3301 cttccaggcc gcgaacggcg tcggcctggt tgtagggagc aatgagatgc agttcgccgt
     3361 tggcgctttt taccagacct ggctggcgcg gaacggcgaa tttttcttta tagggcgagc
     3421 gaataacgcc tatttgctcg aactggaaac tgctcatttc gccgtaatgt taagcgcaga
     3481 gccgatacat acagcctgac gatagcagcc tggcgtaccg ctggtgactt cgcagctatg
     3541 cagtaatacc gcattggctt tcattttaga ggcgttgatt tgcatccgct tacgcgcggt
     3601 tggaatgctc ggcggagagt cctgattaga ggcctggcaa gagtcgccac tgacttcacc
     3661 gagatcgcgg aacggtttgc cgactaattc ttctgcattg gtataaattc ggaccggcgt
     3721 ggcgcgcggc gctttcggtt ttgcaggctc cgctttcggc tggggtgcag tgctttgaac
     3781 gggttcgaca ggggatctgc ttaacatgga acagccgctt agcatgagtg ctactaaaca
     3841 gatcggtaaa gcacgcatag tatttcctca atgtatgatc aaaacgtcaa tattgaatca
     3901 ggagcttgta aaaatgacaa gacgggcaag cgcccgtcct gaatgatatt acaaattgtg
     3961 gaaacagcct aaaaattacc agcctttaac agcgccgccg ttaaacactt tgtttgctgc
     4021 ttcgtaaact tcatcagact gataagcctg gacgaatttc ttcacgttct cggcgtcttt
     4081 gttatcttca cgcgttacga tcaggtttac gtacggggag tctttatctt cgacaaagat
     4141 accgtctttc gctggagtca ggccaatctg gctggcatag gtggtattga taactgccag
     4201 agcgatttgc gcgtcgtcca gagatcgagg cagctgcggt gcttccagct caacaatttt
     4261 cagatttttt gggttctcaa caacatcaag aacggtcggc agcaggccaa cgccatcttt
     4321 cagtttgatc aagcccactt tttgcagcag cagcagtgaa cgaccaagat tagttggatc
     4381 gtttggcacg gcaacctgcg aaccatcctg cagttcatcc agtgatttga ttttcttgga
     4441 gtaaccagca atcggataaa caaaagtgtt gcctactgcg accagtttgt agccacgatc
     4501 tttcagttgc tgatcaaggt acggtttatg ctggaaggcg ttggcgtcga tatcgccttt
     4561 gctcaatgct tcgtttggca gaacatagtc gttgaaggtt accagctcaa cgtccaggcc
     4621 atatttgtct ttcgcaactt tctgcgcgac ttctgctacc tgctgttcgg caccaacaat
     4681 cacgccgact ttaatgtgat ttggatcttt ttcatcctga ccgcagccta ccagtgccag
     4741 tgatccaatc agggctccca ctgccgcaaa ggttttgaat ttgaacgcca taccttattc
     4801 cttcttcaat tatttatgtt gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg
     4861 gtcgcctgcg aactgaatta aataaaccag aatgaccagc aataccagta ccgtattcat
     4921 caccgtcgcg ttatagccga tgtagccata ctgatagcca atctgaccta aaccaccggc
     4981 accgactgca ccacccatcg cggaataacc gaccagggta atcagggtga tagttgccgc
     5041 attcaccaga cccggcaatg cttccggtaa cagcacttta cggacgatct gcatcggcgt
     5101 ggcacccatt gcacgggaag cttcaattaa cccggttggg atctccagca gagcgttctc
     5161 gaccatacgg gcaataaacg gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa
     5221 accaatcgat gtaccaacaa taacgcgggt aaacggaatc atccatacca gcaagataat
     5281 gaacgggata gaacggaaaa tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt
     5341 agcaataatt tgccccggac gcgtgacata aagcagaacg ccaaccggca gaccaatcac
     5401 aaagccaaaa aaaccggata cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag
     5461 cagccacatc atcggctcag acataaccca gtacctctac ttttacatgg tgttcctgca
     5521 gccaggcaat ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc
     5581 cgaacttaac gccaccggcg tagtccatct gcgcgctaat aatgttgttg ttgacgttga
     5641 aacgacgcgc ggtttcagaa agcagtgggg catcgaccga ctgaccggta aactccagac
     5701 gcaacatcgg cacgcagtca gtaaatggct ccgcttgcag acgttcctga taatcttccg
     5761 ggatatccag atgcagggtc gactgaataa acttctgcgc cagcggcgtt ttcggatgcg
     5821 aaaacacttc acttaccgtg tcctgctcga tcagttcgcc attgctgatg accgccacgc
     5881 aatcacaaat gcgctttaca acgtccattt cgtgggtgat caacagaatc gtcaaaccca
     5941 gacggcggtt gatgtctttc agcagttcga gaatagaacg tgtcgttgcc gggtccagcg
     6001 cgctggtggc ttcatcacac agcaatactt tgggattgct ggctaatgca cgggcaatcg
     6061 ccacacgttg tttctgccca ccggaaagat tcgacgggta gctatcatgc ttatcgccaa
     6121 gaccaaccaa tgacagcaat tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt
     6181 ccagctccag cggcagagcc acgttgccaa aaacagtacg cgaagagagc aggttaaaat
     6241 gctggaaaat cataccaatc tggcggcgag ctttggtcaa ctcggattct gacagcgtgg
     6301 tcagttcctg gccatcgacc agcacgctac cctcggttgg gcgctccagc aggtttacac
     6361 aacgtataag cgtactctta cccgcgcctg aggcaccgat aacgccataa atttgtccag
     6421 ctggcacatg caggctgacg ttgttcaacg cctggatggt gcgggtgccc tggtggaaca
     6481 ctttggtgat attcgaaagt tttatcattg attatttatt atcgtcatta agttagtcgt
     6541 ggcatctcga atgcctgaaa cgggcaacgc cgtcaatgaa atggatgtta aggcatccag
     6601 acgtctaaat caatcaggtt tatgcgaaga gcactttctt gcaggtcgaa acatgcgata
     6661 ctagcgtcac atgccttatt aaggagctat aaaaggtggc aaagagcgta cccgcaattt
     6721 ttcttgaccg tgatggcacc attaatgtcg atcacggcta tgtccatgag atcgacaact
     6781 ttgaatttat cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg ggctttgcgc
     6841 tggtggtagt aaccaaccag tctggcattg ctcgcggtaa atttaccgaa gcacagtttg
     6901 aaacgctgac cgagtggatg gactggtcgc tggcggaccg agatgtcgat ctggatggta
     6961 tctattattg cccgcatcat ccgcagggta gtgttgaaga gtttcgccag gtctgcgact
     7021 gccgtaaacc acatccgggg atgcttttgt cagcacgcga ttatttgcat attgatatgg
     7081 ccgcttctta tatggtgggc gataaattag aagatatgca ggcagcggct gcggcgaacg
     7141 tgggaacaaa agtgctggtg cgtacgggta aacctattac gcctgaagca gaaaacgcgg
     7201 cggattgggt gttaaatagc ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac
     7261 cggcataatg attaaaagat gagcaattga aataaaaatg catttttcca cttgtcttcc
     7321 tgagac
//
LOCUS       DAZGXZ010000208         7283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000208 DAZGXZ010000000
VERSION     DAZGXZ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.208"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..2105)
                     /gene="traG"
                     /locus_tag="ACHKJF_004933"
     CDS             complement(<1..2105)
                     /gene="traG"
                     /locus_tag="ACHKJF_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFP0952150.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVAGYEMVFAQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASSDPYTIIFSQPSPLRQVPNNNYSFLQSSEPFVSCK
                     DASVGLKDKLNFDTNTGGKTWNYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSAS
                     QIMRQNVTMNALKEGITSNAARNGDTASLVNLATTSSMEKQRLAHVAIGHVLMRNLPM
                     VQTILVGITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYA
                     KQNGAPVVLSELSQIQLKYSDLAATAGYISAMIPPLSWMMVKGLGAGFSSVYSHFASS
                     SVSPTASAAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRD
                     GNMVMDASGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLS
                     QFGSNRGSSDSVTSGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRST
                     RASAGMYGDAHAEWGIKPKILGVGGGAGVRGGGRAGIDWEDNDAHTASSNTQSSHNAR
                     HDIDARATQDFKEASDYFT"
     gene            complement(2102..3475)
                     /gene="traH"
                     /locus_tag="ACHKJF_004934"
     CDS             complement(2102..3475)
                     /gene="traH"
                     /locus_tag="ACHKJF_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFP0952151.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFATNTTQ
                     AGVWQGQAAGYAYGGSLYARSQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAMGYFFDLALQTTVPEIKTAKDFLQKMANDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKIYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDNREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(3462..3854)
                     /gene="trbF"
                     /locus_tag="ACHKJF_004935"
     CDS             complement(3462..3854)
                     /gene="trbF"
                     /locus_tag="ACHKJF_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFP0952152.1"
                     /translation="MKENKKNQEFKIRKIKRDIERSCDNAIKYFWLFVVFFAAGLIVR
                     NVMHDFFSAGIDSWKADPELNNFRYMWNTLMYVIPIMLYALAAGFLAAASLSPLCEII
                     FGGVRIFLLKRRMRRENTLREGSNNASH"
     gene            complement(3808..4122)
                     /gene="trbJ"
                     /locus_tag="ACHKJF_004936"
     CDS             complement(3808..4122)
                     /gene="trbJ"
                     /locus_tag="ACHKJF_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFP0952153.1"
                     /translation="MRNKQVVLLIAGISGVATGIIVSLNIPFIRQGLFYPASPVEIVV
                     SLCLTFSVSVVFFVGAIVGWILVSEIYYSRMTGLNESSEISEETYNERKQKESGIQNQ
                     KN"
     gene            complement(4112..4666)
                     /gene="trbB"
                     /locus_tag="ACHKJF_004937"
     CDS             complement(4112..4666)
                     /gene="trbB"
                     /locus_tag="ACHKJF_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFP0952154.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMAAQPAQPAA
                     DTSARTDKTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYG
                     FSVFPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGA
                     TDAAGFMARMDTVLQMYGGKTGAK"
     gene            complement(4653..4934)
                     /gene="traQ"
                     /locus_tag="ACHKJF_004938"
     CDS             complement(4653..4934)
                     /gene="traQ"
                     /locus_tag="ACHKJF_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001732192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFP0952155.1"
                     /translation="MSKYKFSLPRLDITGVWVFSLGLWFHIVARLVYRKPWMAFFLAE
                     LIAAILVLFGAYQILDTWIARVSREEREALEARQAAILAAEKESRHVSH"
     gene            complement(5000..5401)
                     /locus_tag="ACHKJF_004939"
     CDS             complement(5000..5401)
                     /locus_tag="ACHKJF_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001477376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952156.1"
                     /translation="MDKENSSIPERWLIASGVAVILLTLLAFMSFPEPDVSMNRYYSW
                     LMNVLILSVTGVMYVALSVVMVILYFLYERNSRLLYLSVLALIIAAGIWLLRFLDDFL
                     MTPAFHVDLFVLWVLLCFLYIRCKNEMSIPE"
     gene            complement(5532..5897)
                     /locus_tag="ACHKJF_004940"
     CDS             complement(5532..5897)
                     /locus_tag="ACHKJF_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952157.1"
                     /translation="MNFISLWPLLLAQVTVMSLTVIFLAADRRFFSWDCFVVCSLSMM
                     MAEGIVIMKYELWHIPPALLGLAGVLSWLLLLLHTGYFSGISEGVHKRLWLAVASVFS
                     GAGMIIFYWLLQPSETIMK"
     gene            complement(5911..6654)
                     /gene="traF"
                     /locus_tag="ACHKJF_004941"
     CDS             complement(5911..6654)
                     /gene="traF"
                     /locus_tag="ACHKJF_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFP0952158.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKMKQPEPENKPAPVI
                     PRQEPDIMQKLTALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEQYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(6647..6904)
                     /locus_tag="ACHKJF_004942"
     CDS             complement(6647..6904)
                     /locus_tag="ACHKJF_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFP0952159.1"
                     /translation="MKVIFTSNRLVDFLIRLLITAIVISPVIIWSWDTVKETTAGGVL
                     AAAFVIFYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(6918..>7283)
                     /gene="traN"
                     /locus_tag="ACHKJF_004943"
     CDS             complement(6918..>7283)
                     /gene="traN"
                     /locus_tag="ACHKJF_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HFP0952160.1"
                     /translation="EKALGKAKDNKLTVSVGEFCSRKVLGVCLQKKRSYCQFDSKLAQ
                     IVQQQGRNGQLRIGFGSAKSPDCRGITVDELQRIKFDQLDFTNFYEDLMNNQKIPDNG
                     ALTEKVKEQIAGQLKQVGQ"
BASE COUNT         1893 a         1963 c         1678 g         1749 t
ORIGIN      
        1 ctggtaaagt aatcgctggc ctctttaaag tcttgtgtag ctctggcatc gatatcatga
       61 cgagcattat ggctcgactg agtattacta cttgccgtat gcgcatcatt atcttcccaa
      121 tcaataccag cccgaccacc acctctgaca cctgcgccac caccaacacc aagaatttta
      181 ggtttaatcc cccattcagc atgagcatct ccatacatcc cggcagaagc tctcgtactt
      241 ctggatgcca gctcctgagt cgcctgctca ttactgatat tatgcgcctt cgcatagctt
      301 tccactgcgc tgcgcatacg gctggccatc atggaatcct gcgcgctcat cgtactgtca
      361 gcgccgctgg tgactgaatc actgcttccc cggttagaac caaactgact gagcgagttc
      421 caggcactgg caatactgct gctgaaaccg tgcagggcgc tttcagctct gttcgacgcc
      481 tcccgggcca tttcctgttg tgccgccgca atctgacgcg ttgcattgat accgaccggt
      541 aaacgggaca tcgctccgct agcatccatc accatattac cgtcacgggt ctgtgtggcg
      601 gttgcgccac ttccggtctg gtacatcatc tgaccaaacg acgtggtgct gttggtgctc
      661 cagctgaagc cgttcacgtt ctccgtctgc atgttgccgt aggagtaatt accgtcaacc
      721 acactgcctg ccgcactggc agtcggactg acagaggaag aggcgaagtg gctgtacacg
      781 ctggaaaaac ctgcgccaag gccttttacc atcatccatg acaacggggg tatcatggca
      841 gagatatatc cggcagtggc cgccaggtcg gagtatttca gctgtatctg agagagttca
      901 gagagcacga ccggtgcacc attctgcttt gcatagaatg tcatggcact gttcagaatg
      961 gcatacagca acggccaggt ctggaaccac atcagggcaa acacatagcc tctcagaaca
     1021 gacagcgtca gtctgttgaa gacagctgcc agtaccagaa gcgggaagat accgatggta
     1081 atgcccacca gaatggtctg caccatcggc aggtttcgca tcagcacatg accaatggct
     1141 acatgcgcca gacgctgttt ctccatcgat gaggtggtgg ccagattcac caggctggcc
     1201 gtatcaccgt tacgggcagc attactggtg ataccctctt tcagggcatt catggtgacg
     1261 ttctggcgca taatctggct ggcagactgg ctggagccgt agaaataact gtaactgtca
     1321 ctcaccagtt gtctgaataa caggtcaggg tccggcctgc cgccaaatat ctgctgcaca
     1381 taataattcc aggtcttgcc tccggtattc gtatcaaaat tcagcttatc cttcagtccg
     1441 acggatgcgt ccttacagga cacaaacggc tctgagcttt gcagaaaact gtaattatta
     1501 ttcggcacct gacgtaaagg gctgggctga gaaaagatta tggtgtacgg gtcagaggag
     1561 gccatcaggt cttccagcgt gtatttgtgg ttcaggtaaa tatcacccag cacgcagttc
     1621 tggacgtagt cctgaaacag attgatgatt tccggattcc gggacaggaa atcggtgctt
     1681 ttcacaatca ggttcgcccc gaacagcatg cctgtcttgc tgtaggtggc gctgtcaggc
     1741 tgagcgaaca ccatctcata accggccacc atcgcatgcc cgatacgggt cgtcagtgaa
     1801 agtggcatcg ccagtccgac cggcacatta tccacccggt gaactttgac caggtcactg
     1861 ttatcaataa tctgcaccga tgtacggaca ttaaccagca ggctgataag cacaaacacg
     1921 gccacccagc ccagcaaatc catcacgttg tgccgctgta cccacatcac ggccaccgcg
     1981 agaacagaca atgtgagcgc aattttttcg atggaatccc acgtccgggt ccccataaag
     2041 gcggcaatgg cgttcaggtt attgcgtaac cactcaccac cggcaatcac atagacttca
     2101 ttcacagcgt gctccctccg aagtgatagt tgttctggta acggctgagc atgcgggcgg
     2161 acagctgctg gcgcatgtag ctcatctgac ggtcaacaac cagcaatgca tcctgctgga
     2221 cctttacctg cccctggaaa accgcaatct gttgctgtgc ctgattcaga ttctccagca
     2281 cactgtccag cacggtctgt ggatagttcc cggtggcaat catggcccgg gcctgctgaa
     2341 gcagttcctg aatgtactgg agcatgatgt cgtagccgat atagtccgtc agctggtaaa
     2401 ccacgctgct ggacacaccg agcatctgcg ggtcaatcag atatttaaag acggggacgg
     2461 tggtgctgga aatgaagcct ttttccctgt tatccagtgg agtatcactc accgctttat
     2521 tctgaatact ggtgagcagt tttgtaatct gtgatttcag ggctttatcc cgggcgatgg
     2581 tcacatttgc atccgccacc accttcagac acttgtccga atcgttgcag tggtaaattt
     2641 ttgccgttcc cccctccata atcgcccgga ggatatcgcg gtcagtggta cgcgccggca
     2701 ggggggtaat ctcgctgtct tccccaaaaa tcagcgtacc ggtgagcgtc ataatgaact
     2761 ctttcagctc tttgttacca tcaagcatcc tgtttttgga cagcgagtcc cagatgatat
     2821 tgatgttctt cagcacccgt tccttatctt tgtcactggc cttgtcacga acagacgatg
     2881 actgtcctcc gaccgtgcag ccctgacggg acgcggccca gtcggcgaaa atattgctct
     2941 caccggcaat atcctgacag accttctgct gtgacacctg agtctgagga aataatccac
     3001 cgacaatccc ctgtgccacc tgacaggagc tgaggttcat gctgttaatg tcattcgcca
     3061 tcttctgcag gaaatctttt gccgttttga tttccggcac cgttgtctgc agggcaaggt
     3121 caaaaaagta acccatcgcg ttactcataa tctgcttaac aaaacgctgc agctgctcgc
     3181 cattaataaa actgaacgag ccgagatagg catcgatgcc gccacaccct gcattgatgt
     3241 ccggcagtgt catggagata agctggatat ttttgacctg gctgcgggca tagagggagc
     3301 caccataggc atacccggca gcctgcccct gccagactcc ggcctgggtg gtattggtgg
     3361 caaagcccag cttgttaaag aactggttca tgtcgctgtt cacatccgct gacgctgcgg
     3421 gtgtgaccgt cagtaaggcg gcacaaagaa taaaaagagg cttaatgcga agcattattg
     3481 ctgccctccc ttaatgtgtt ctcccggcgc atacggcgtt tcagcaggaa tatccggaca
     3541 cccccaaata ttatttcgca caacggcgac agagaagccg cagcaagaaa accagctgca
     3601 agcgcatata acataatcgg aatgacatac atcagggtgt tccacatata acgaaaatta
     3661 tttaattccg ggtcagcttt ccacgaatca ataccggcac tgaaaaaatc atgcataacg
     3721 ttccttacaa tcaatccagc agcaaagaaa accacaaaaa gccagaagta ttttatggcg
     3781 ttatcacaac tgcgctcaat atcccgttta atttttctga ttttgaattc ctgattcttt
     3841 ttgttttctt tcattataag tttcctctga tatttctgat gattcattca gaccggtcat
     3901 gcggctgtaa tatatttcag acaccaaaat ccatcccaca attgccccta caaaaaacac
     3961 aacagaaaca gaaaaggtaa gacacagcga aacgacaatt tccacagggc tggcgggata
     4021 aaaaagcccc tgacggataa aggggatatt caggctgacg attattcccg tcgcgacgcc
     4081 ggatattccg gcaataagta acaccacctg cttatttcgc acctgttttc cctccgtaca
     4141 tctgcagaac ggtatccatc cgcgccataa aaccggcggc atccgttgcc ccctgtaaaa
     4201 gcggtaatgc ctcaagcgtg ttgacgttga ccaggaaggt ggtcggtgtg gccaccggaa
     4261 tattcgggaa gaaggtctgc atcacgtccg gtggcaccgg taatgcttcc ggaaaggcgg
     4321 tatccccctg accatccagg gtgtagggaa aaacggaaaa gccgtactgc tgtgccagct
     4381 gcttcagtac cgggtcaaac tggtgacagt aagggcaatg cccctgcata aacagcacca
     4441 ccttccagtc ggccaggtta acctgcctgc cgttgctgag gcggaaccag cggggagccg
     4501 gcttttctgt cttgtctgtc ctggctgacg tatctgctgc cggttgtgca ggctgtgcgg
     4561 ccataccctg cggattccag agtcgctcta tttcatcccg ggtggaggcc tgcacggcag
     4621 cggctgacag taacagggtg aacagcagtg atttagtgag agacatgtct gctctccttt
     4681 tctgcagcaa gaatggcggc ctgacgggct tccagtgcct cacgctcttc ccggctgacg
     4741 cgggctatcc atgtatccag tatctggtat gcgccaaaca gaacgagaat ggccgcaatc
     4801 agctctgcca gaaaaaaagc catccagggc tttctgtaaa ccagacgggc aacaatatga
     4861 aaccagagcc ccagggagaa cacccataca cctgtaatat caaggcgggg taaagagaat
     4921 ttatatttac tcataaatat atcctgtttg taatatctgc cacactcagc aggatggcaa
     4981 ataaaaaacc gactgattat tattccggta tactcatttc atttttacag cgaatgtaca
     5041 aaaaacagag gagcacccat aaaacaaaca gatcaacatg aaatgctggc gtcattaaaa
     5101 aatcatccag aaatctgaga agccagatac ctgctgcaat gattagggca agcacagaca
     5161 agtacaataa cctgctgtta cgctcgtaaa gaaaataaag tattaccatt acaacagaca
     5221 gcgccacata cattaccccg gtcacactca gaattaacac attcatcaac cagctataat
     5281 agcgattcat actgacatcc ggctccggaa aactcataaa agccagtaag gtcagtaaaa
     5341 taaccgcaac accagacgcg attaaccatc tttcaggaat actactattt tctttatcca
     5401 tacagaataa aaccatactt aacaaacaac atgaattaaa agccattcac catcaggtgt
     5461 ggggacagaa aaaagttatc tctgattgtc ttttactctg taaatgagtt catttacata
     5521 ttaacagggc attatttcat tatggtttct gatggctgga gtaaccagta gaatataatc
     5581 attcctgcac cagaaaacac tgaggcaaca gcgagccaca aacgcttatg cacgccttct
     5641 gatatgcctg aaaaatatcc tgtatgaagt agcagcagca accatgacaa cactcctgcc
     5701 agacccagca gggcaggagg gatatgccac agttcatatt tcataataac aataccttct
     5761 gccatcatca tggacaggct gcaaaccaca aaacagtccc atgaaaaaaa acgcctgtca
     5821 gcagcaagga aaatcactgt cagtgacatc actgtcacct gggccagcaa taaaggccat
     5881 aaagaaataa aattcattta aacgacctct ttaaaaattc ggtttaaagt cttcagaaac
     5941 gttcaggaac tgttttgcca ggtcgtcctg agaaataaag ccgtatgata acgggcgaac
     6001 actgccctgt ttcgggtcaa ccagcatcat ggccgggaaa tatttcacac cgaggcgctg
     6061 cgcctgcccc tggtcagtcc gggaatccgg caacagtgga ttaatcacgc catccacgga
     6121 aacgggaata acactcagac catacgtatc ccggaagcca ttaatgacct gcgccagctg
     6181 cccgtcgatg gggtcctgcc cccggtaaaa aaacataatg ccgtattgtt cagccagttt
     6241 cgcaatggcc tgtcgctgct gtgcctggtc tgccgccagc tggttccgta ccgtgccgtt
     6301 gtaatggctg tactgcaggt tatagtccag ttcaggatgt gccagcatgg cctttttggc
     6361 gctcatggtg aaaagcccgg cctgctgagt ccagtaattc tgcagccgga aatatttcac
     6421 aaaattatcc actccgggat acagaatggc ttcgtacagc gcccgctttg ttgccgtctg
     6481 cagcgcggtc agtttctgca taatatccgg ctcctgacgg ggaattacag gtgcaggttt
     6541 attttccggt tccggctgtt tcatcttttc gttataccac tgccagcctg catctttccc
     6601 tgacgccgga aaaataaagc agcagagtaa cagtggcagt aatgctttat tcatcttcat
     6661 tcctttcatc actttttctt tcatcaggtt tctgcaggtc agtcagagca gaaaaacaaa
     6721 aatacagaat aaacaggagc acaccggagt aaaaaataac gaaagcagca gcaagcaccc
     6781 cgcctgccgt ggtttctttt accgtatccc atgaccagat aattaccggg gaaatgacaa
     6841 tcgctgtaat caaaagccgg ataagaaaat caacaagacg attcgacgtg aatatgactt
     6901 tcattatttt cccgacctta ctgccccacc tgtttcagct gaccggcaat ctgctctttc
     6961 actttttcgg tcagcgcacc attatccggg attttctggt tattcatcag gtcttcgtag
     7021 aagttagtga agtccagctg gtcgaactta atacgctgca gttcatccac ggttattccc
     7081 cggcagtcag gactttttgc gctgccaaac ccgatacgca actgcccgtt acgcccctgc
     7141 tgctggacta tctgcgccag cttcgaatca aactggcagt aactgcgctt tttctgcaga
     7201 cagacaccca gcactttccg ggagcagaac tcgcccacac tcaccgtcag tttgttgtcc
     7261 tttgcctttc ccagcgcctt ttc
//
LOCUS       DAZGXZ010000209         7218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000209 DAZGXZ010000000
VERSION     DAZGXZ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.209"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(97..438)
                     /locus_tag="ACHKJF_004944"
     CDS             complement(97..438)
                     /locus_tag="ACHKJF_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952161.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVR"
     gene            complement(431..3673)
                     /locus_tag="ACHKJF_004945"
     CDS             complement(431..3673)
                     /locus_tag="ACHKJF_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0952162.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQDGGRQAA
                     AAAREQRRALAELNSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASAAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     TMADDGQLTADKVVPALISQLGILRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     ATKTLSGALNGVAGNIDTVATAAGVLVAVGVARYFGNLASGAMSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAVTQERLNRNIAART
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGNGFMINHLTSVKTVTEELAQATEQFAVEQSRLAQMQEKAQSIQDVLAGLEERR
                     VVLIRQQAAEQNKAYQSLLVMNGQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLD
                     DKQQGALNNTERQLALSRLKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGKNTELAKLKYQTSQGELKT
                     LTEIQKQELLRNAALIDQKKIRVLLRAREETLKNDNADARASNDAELLGYGQGERVRE
                     RMRELQQIRDGYRQKDANLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAES
                     DAQRADWQKGLQEGLSNWVDSASDYASQAAQLATDGISGMVNNITEMLNGNKVEWRSW
                     AASILQEISKVLMNVAIVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAVGTLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(3721..3930)
                     /locus_tag="ACHKJF_004946"
     CDS             complement(3721..3930)
                     /locus_tag="ACHKJF_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP0952163.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            complement(4026..4400)
                     /locus_tag="ACHKJF_004947"
     CDS             complement(4026..4400)
                     /locus_tag="ACHKJF_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP0952164.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     REVLDGGDAGDEPLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4415..5131)
                     /locus_tag="ACHKJF_004948"
     CDS             complement(4415..5131)
                     /locus_tag="ACHKJF_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0952165.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKYLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(5197..5541)
                     /locus_tag="ACHKJF_004949"
     CDS             complement(5197..5541)
                     /locus_tag="ACHKJF_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP0952166.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVAVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5538..5984)
                     /locus_tag="ACHKJF_004950"
     CDS             complement(5538..5984)
                     /locus_tag="ACHKJF_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP0952167.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGIHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(5981..6331)
                     /locus_tag="ACHKJF_004951"
     CDS             complement(5981..6331)
                     /locus_tag="ACHKJF_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP0952168.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6341..6667)
                     /locus_tag="ACHKJF_004952"
     CDS             complement(6341..6667)
                     /locus_tag="ACHKJF_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0952169.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELIQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(6664..>7218)
                     /locus_tag="ACHKJF_004953"
     CDS             complement(6664..>7218)
                     /locus_tag="ACHKJF_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0952170.1"
                     /translation="LEQMVKEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQDGR
                     DATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTG
                     ERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTGD
                     KPGEAHSSGWTLAAKRGRDAGGGK"
BASE COUNT         1493 a         2403 c         1693 g         1629 t
ORIGIN      
        1 caaaatgtaa atacaagatc gatttcgcac tttctgacaa gacagattaa aaataattaa
       61 caactaaaca ataaagagtg atttatagga taattctcat ctgactacct gctctatcgt
      121 gcaactgaaa tcactgtacc gggcgttatc cgtaatactc cactcccggc acaccaccct
      181 gaccgtccgg ttatgtttcg ggggcttcca caaaaaagcc cggtaaccac catgccatga
      241 cagaaacgcg gacagtgcct cccgctcagc atccgttgtc acccgaaaaa ccacctgaaa
      301 ggtcttcagt tgcgcattca gtcctgccgg acgacgttgc tgataaccgt cgccaaactt
      361 caccgtcacc accgacggtt tctccgtcac ctgcatccct tcccggggac accagtgaag
      421 ggtcttaatc tcatccactc agcattcctc cgtcacgacg catggataac atcaccgcct
      481 gtacccgctg gtcaatcagt tgcacaaggg tgcccaccgc ttccggccct atctgcccgt
      541 tagcgccgtc attctgaatg gcaatgtggt acaccgggga atacaccaga cccgcactgc
      601 cgttcatact gcccaccgca cgcacgccga gtgaaccatc cgctgcccgg gtcaggggca
      661 tgatggcttc aggtccggct tcccccatca gccctgcccc ttttgcaaag gcaaagtacg
      721 tgggcgtgtc cacaatactg ttactgtatg cactcaggtt tgccgaggta taaacgccgc
      781 cttttgcatt tgccaccgcg ccacccagcc agttgcctat actgccgaaa aatcctcctg
      841 caccggacat actgtttgcg gccatcttaa tgccgttgac aatggccaca ttcataagaa
      901 cttttgatat ttcctgcagg attgatgcag cccagctgcg ccattccact ttatttccgt
      961 tcagcatctc cgtgatgtta ttcaccatcc ctgagatacc gtctgtcgca agctgtgctg
     1021 cctgtgaagc gtaatctgat gcgctgtcca cccagttact tagcccttcc tgcagccctt
     1081 tctgccagtc agcacgctgc gcatccgatt cggcataaaa agcctcctgg tctttaaggc
     1141 gttcactcag atactgcgca ttctgcgcca gcgcctgtct gtaaaaatcc tcactgatat
     1201 cccctgtctg atactgggac tgcaggttcg catccttctg gcggtagccg tcacgaatct
     1261 gctgcagctc ccgcatccgt tcacggaccc gctcaccctg cccgtacccc agcagctcag
     1321 cgtcatttga tgcccttgcg tctgcattat catttttcag ggtctcctcc cgggcccgta
     1381 acagcacccg gattttcttc tggtcaatca gggcagcatt gcgcagcagc tcctgcttct
     1441 gtatctccgt cagggttttc agctctccct gcgaggtctg gtacttcagc ttcgccagtt
     1501 ctgtattctt ccctgccagt gccagttgtt ccttctgctg cttcagcaga cgggaaaaac
     1561 tgtcttccgc tttttccgtc tcagattttc cgccccggga tttgggttta ttcgcctcat
     1621 tgttgcgcca ggcttccatc gaattacgga tgtaacgctg cctcgcctcc tgatacgtat
     1681 cccccaccag accaaggtca tccgccgcat accccagccg ggcacgctct ttttcttccc
     1741 ctttcagccg ggacagggca agttgacgct ctgtgttatt cagggcgccc tgctgtttat
     1801 catccagagt ggcctgtggc agtcgcagcg gtacactcgc cagtccctga cgctgttgca
     1861 gcagttcatt acccagcccc agcaggcggt tgaattccgt atgctgaccg ttcatgacca
     1921 gcagtgactg atacgcttta ttctgctctg ctgcctgctg acgaattaac acaacacggc
     1981 gctcttccag tccggcaagc acatcctgaa tggactgcgc tttttcctgc atctgtgcca
     2041 gacgggactg ctcaacagca aactgctctg ttgcctgagc aagctcttcc gttacagttt
     2101 tcaccgatgt cagatggttt atcataaagc cgttaccggt cgtccagccc ggattagcca
     2161 gcacatactg atacccggcg attttttcct gcagggattt cacccggctg gcctgctcat
     2221 caatcagccg gttctgctct gtcagcgcct cccgtgttcg cccttcatta tctgaggctt
     2281 caggcagaga cattgatggc gttttatgcg cgatttcatc aatcgtcagt gcatactggc
     2341 gcgcagactc cctggcctgt tcctgattct ggtacagcgt gtaccatgcg gcagctccca
     2401 gcatcaccag tccgggtacg cctccaacca gtcccagcgc accgctcatc agacgtgagc
     2461 ccaccgccgt tgtactgttc agcgcattct gggcggcggt tctggcagca atatttctgt
     2521 tcaggcgttc ctgtgtgacc gccagacggg cctctgccgc aatctgcatc tcagtcccgc
     2581 gggctgccgc cacagcctgt tgagcccggt acacggctgc ccttgcccgc gccgtggcaa
     2641 tctgcgttcc ccttaactgg gcctcagcca gtgccacttc attacgtgca gcggtcacaa
     2701 gtcctgccgt ggcagacatc gctccggaag ccagattacc aaagtaccgg gccaccccga
     2761 cagcaaccag cacacccgca gctgttgcca cggtatcaat atttccggcc acaccgttca
     2821 gtgcgccgga gagcgttttc gtcgctccgc tggcctcatt cgcgccgccc acccaggcca
     2881 taaaggcgtt ttccaccttc gtgatcccgt cagacaccgt ttccggcatg gctgcatatt
     2941 catcacgcaa tatccccagc tggctgatta acgcgggaac gactttatcc gccgtcagtt
     3001 gaccatcgtc cgccatcgtc ttcagatcct tacgggccac gcccatgcct gcagccagtg
     3061 cgcgaatgat ccggtcccca ctttcattga ccgaattaaa ttcctcgccg cgcaacaccc
     3121 cctgtgccag cgcctggctg aactgggtga tcaccgaacc cgcctctgcc gcactggcac
     3181 cggaaatttt cagccctgtc gaaatggcct ccgtcacctt cagcacatca tcagcactgt
     3241 aaccatattc acgcatcgag gcagccgaac gggcaaacag agccgcatta tccgaaaatg
     3301 cggtgcctgt ccgctggctg atatccatca gcactttctg tgatgacgaa aattcatccg
     3361 atgactgcga cgcctgtttc agacgggcat tcacggagct ccattcatcc gccagcgaaa
     3421 tcaggtgtcc ggtggcaaag gcaccggcaa acgcaccggt cattccgaca gccgaagcgc
     3481 ggatttccgt caactggctg ttcagctcag ccagagcccg gcgctgctcc ctggctgccg
     3541 cagcagcctg acgtccgcca tcctgcaggg tccggtaata ttcactgccc atacgggacg
     3601 cccgctggat ctccgactgg aatgactgtg aatttgccga aattttgata atcagttcac
     3661 gtaacgtcgc cattcacctt tctccgggcg taaaaaaacc gcctcagcgg ttctcatcat
     3721 tcatgactgt gctgcaaagc tcagcgcgtc ttccagcgcc gcaaacggat ccacctccgg
     3781 cttatcctca tcctcgcccc agcagagcat ggcgtccttc agtgcaacat tcatcccctg
     3841 tgccccgaaa accgctttca cgatctgtgc attacggata tccccgcgct catcacccaa
     3901 cggggatacc ctgtcaaact ccatccacat catcgcctcg ctcgcactca ggctgtgccg
     3961 cagttcggat aaggtgcgcc ccagacggag cgcaagtcgc atcagaaagc gaatttccgg
     4021 gcgggctact tttttctggc cgactctgca tcagcgatca gttccagtgc ctgacgcagc
     4081 aaccgggcat gtaccggacc atagacggcc agcacctgct cacggtcgtc cggagtgaac
     4141 acccgttgca ggtccgtatc acacaggaca tcgcagaaca gcgtcacatc cgcttccagg
     4201 ttacggcggg ttttcgccac caccgacagg ggttcgtcac ccgcatcacc gccatccagc
     4261 acttcccgcc acagatacca ggcctctgcc gaaggctccc gcagcaccac gctgacatta
     4321 ccccattccg gcaccttcac cgttttatga cgaaaccctg acagtctggc cagcgccagc
     4381 gttttcagat cctttttcat gatgccccat ccccttatcc ggcggctgcg ctcactgtca
     4441 cggtacattc aacagacgtc acactctgtg ctttctctgc cgaatcggtc accacgcagg
     4501 tatatttccc cgcatcagcg gactgcgcac ctggcttact gaaggtgtct gtcgtctgcc
     4561 cgtcaaccgg ctgaccatcc ttcttccagg cgtatttata cggcggcatt cccccgttgg
     4621 cactgactga cattgtcagc agcgcaccgg tattcacggt aagtgtctta tccagatttt
     4681 tcacaaacgc cagcggtacc acaaaggaca ccggtttgcc tttcagacgc agtgagaacg
     4741 ttgcagccac cacgccgttg gtaccggatg accaggtgtg ctgacgcact tccgccaggt
     4801 atttaaagcc cttaccggac ggaaactgca ccttaaacgc atacaacgcg tcattgtcat
     4861 aagcatcacg cagggcgttc tgggcctgat tcagataaaa attacccgac atggaaatct
     4921 cagacgacgc ccccagaccg ttgatgttct cctgctctgt ggagcagagc gtggtcacat
     4981 caatatcctg tttctgaccg gcggtgaact ggacttcctt gatggtgcag tccaggcgca
     5041 gatattccgc cttatccata gtttcagcag tcgccggggc agatgaaatc atcacctgcg
     5101 tcagctgtga gcgttcatac aaagcagaca ttctgcctcc tgataataaa aaacccgcac
     5161 gcggcggggt atgggttttg tagaaaaaag aaaaagtcac accgtgacct gaaactccag
     5221 ggttgcacgg taacagcggt tttccggaat atagtcctgc atttcactga cggatcccgg
     5281 ggccagcagc attatggctt cacgggcgtc ctgacgtatc tgacgcgcct gcgtcacagt
     5341 cccggcataa acgtctatct gcaccgccac tgaggactct gcctgcccgc ccatcacgtc
     5401 cgcagacacc gatgaaatca ggctgaaaac cacccacgga agcgccaccg acggcctgcc
     5461 atccagcagg gggaccacat acgggtacac ctgcccgccg gcaagatgcg ccagatgagg
     5521 atacaaatcc gcctccgtca tcgtctcagt acctcatcaa tggcccggtt catccgagca
     5581 atcgccacct gcgctgcctg ttcactgcgc acatcaaacg ccgggcgcac aaacgggtgc
     5641 ggtggcatat tcacggttcc catttccaca aaccgccagt agaaagcatt gcgcgggtta
     5701 tccgccttca tggtgttatc gctgttaccg gtgtccggat taacaccacg gatatggata
     5761 ccggattcca tcccgccatc gcgggagcgc cgggaaagga ccaccacatt gcggcgcagt
     5821 tttcccctgc gcaccggtgc ccgtgacacc acttcttctt tcagcacatt cgcacccgca
     5881 cgggttgcct cacgcagcac ccggttattt tccgcaccac tcagaagctg caaatcgcgg
     5941 ctgatgtcct ccagccccga aaaatccagc agggtttcga tcatttttcc cctcccagcc
     6001 gacagagaat ttccagacgc ccgccggtcg catccggcac aggcagcccg acaacgttca
     6061 ggatccggtc acgccaggga ccactcagca catgaagtcg tgacgctgcc gtgatttccc
     6121 gaccggactg accgcgcacc cagatgcgga tttccgcctg cgccatttcc gcaccggact
     6181 gcatccgctc ccggctgctc ctgccacgga tatccgcatg aattttcccg catgacaccc
     6241 attcttccgt catttctccg gcagcattac gggttaacac cgggttcaga acacttatca
     6301 tctgtgtcag acgacctgca gatattgcca ttccccctcc tcataacacc gtcggacaac
     6361 gcaaatcgta aatcagcacg gaaacagaaa acggcagctc cccctgaatc agttcttccc
     6421 gctccgcaag atccggattc cggtacagca tcccggtcag tcgcatggca gcccccttca
     6481 tccgggttaa tgcctcgccc gggatcagtt caccgtcctc acgaatcacc ttatcccggc
     6541 tgccctgaat gtaggccagc agcacggcgg tagcctgacg aaccttgtcc atcagcatgt
     6601 catcatccgc gtcatggtcg acacgcagat gtgccttgat ctcttccagt gtcagtaatg
     6661 ccgtcatttt ccgcctccag catcccgccc acgttttgca gccagggtcc agcctgatga
     6721 atgagcttct ccgggtttat caccggtcat actgttgcag tgccacagcg agccgcccca
     6781 cgtcaccgta tcgccggggt ggtaggtttc accggctctg aacacaccgc ggtagagcat
     6841 caccggcagg gaaaatgttt tttccgtacg ctggccactg ctctgccgga ccaccacaga
     6901 gaacaaccgt tcacccgtca tgctgacgtc aatatccgcc accccgtcaa ccaggcattc
     6961 ccatccccgc atcccgtgcg ttttttcata cgcccgccag agtccgccct ggtgtgtggc
     7021 atacgtgccc cggggaaagg atttttgatc gtcaatggcg gggagtattt ccagtgccgt
     7081 ggcatcacgc ccgtcctgcg gagccggcag ggcactcacc gcatccagaa cagccttctg
     7141 cagaacatcg ggatcatagt cacgaccatc acgcggaaca ggaatatggc ttaccgcctc
     7201 cttcaccatc tgttcaag
//
LOCUS       DAZGXZ010000210         7192 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000210 DAZGXZ010000000
VERSION     DAZGXZ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.210"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..372
                     /locus_tag="ACHKJF_004954"
     CDS             <1..372
                     /locus_tag="ACHKJF_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952171.1"
                     /translation="FDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHA
                     RLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILNTLNKMAESFGFTDNPR
                     YIAEKNYMEALLKKASPHTVR"
     gene            complement(341..2077)
                     /gene="aceK"
                     /locus_tag="ACHKJF_004955"
     CDS             complement(341..2077)
                     /gene="aceK"
                     /locus_tag="ACHKJF_004955"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP0952172.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2358..3662)
                     /gene="aceA"
                     /locus_tag="ACHKJF_004956"
     CDS             complement(2358..3662)
                     /gene="aceA"
                     /locus_tag="ACHKJF_004956"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP0952173.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(3692..5293)
                     /gene="aceB"
                     /locus_tag="ACHKJF_004957"
     CDS             complement(3692..5293)
                     /gene="aceB"
                     /locus_tag="ACHKJF_004957"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP0952174.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(5562..6491)
                     /gene="metA"
                     /locus_tag="ACHKJF_004958"
     CDS             complement(5562..6491)
                     /gene="metA"
                     /locus_tag="ACHKJF_004958"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP0952175.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6648..7091
                     /locus_tag="ACHKJF_004959"
     CDS             6648..7091
                     /locus_tag="ACHKJF_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0952176.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         1736 a         1843 c         1858 g         1755 t
ORIGIN      
        1 ttcgataaaa aaaatatgaa gcccctcctc ctggcaaata attctaatga atatcccggt
       61 ttgttttcag cgatacagca taaacaacaa aatgttgtag agacggttta tcttgcttta
      121 tctgaccatg cacgcctgtt tggatttacc gctgaagata ttatggattt ttggcaacac
      181 aaagcgccac aaaaatactc tgcctttgag ttggcttttg aatttggtca ccgggttatt
      241 gctgaattaa tccttaatac attaaataag atggctgaaa gctttggctt tacggataac
      301 cctcgataca ttgcggagaa aaattatatg gaagctttac tcaaaaaagc atctccccat
      361 accgtacgct aaatctttgc ctgcgccgat acgcataaac atcttccaca tgcccttcac
      421 gtatgcggtt ttgtagtgcg cgccagtaat cagcgcggaa caggtcggcg tgcatctctt
      481 caaacagcgg accaatacgc gggtcggcgc atagccagtg gcgaaactct tccgggaaaa
      541 catcgcccgg cgagacgctg taccacggtt cgctggcaag ttcgtcttcc ggatagcgcg
      601 gcggcgggat gtcgcggaaa ttcacttccg tcatgtagca aatttcatcg taatcataaa
      661 aaaccacacg cccgtgacgg gtgacaccga agtttttaaa cagcatgtcg ccaggaaaaa
      721 tgttagcagc ggcaagctgg cgaatagcgt taccgtattc ttcaatggcg tcgcgcaact
      781 gctgaccttc cacttgttcc agccagatat tgagcggcac catccgccgc tcgatataga
      841 gatggcgaat tacaatctgc tcaccaagat cggtgatttt ttccgctgct tcctgaagca
      901 gtaattccat taatgccggg gaaatatgcc gcttctccag cacaaagttt tcaaactcct
      961 gggtgtccgc cattcggccc acacggtcgt gctctttcac cagttgatag caggcacgaa
     1021 cgtgagcggc agacatctct ttctgcggcg cgaacttgtc tttgatgact ttgaataccc
     1081 gatcaaagcc cggcagcgta aacaccaaca tcaccattcc acgaataccc ggcgcttcaa
     1141 tgaactgctc attacagccc tgtagataaa cgagatattc gcggtagctt tcggttttgg
     1201 cgtgcttctg gcagccgata gccatataca attcagcagt ggttttacct ggcagaattt
     1261 cccgtagcca ctcgaccagc gctgcgggca gcggtgcata aaccataaaa taagaacgcg
     1321 caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag gcaggtatca ataaataact
     1381 cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa cgtgccggaa ggcgtgatca
     1441 gtttgcccac cagccaggcg gctttattgc ggtaaaacag ttcgttcgcc acctgtaaat
     1501 gactttccgc gaggttgtct gtccccagcg tttccgtcag atggcgaatg atgtaatgaa
     1561 tgtcacggct tttattctgc cagcgcaggc gcagcggcag gtcgctgata acgcgcatca
     1621 gtagagattc ccagccgtga tcggggtgaa agtctttcgc cagcgggcgg ggaatggtac
     1681 gaaagcggcg ctctggctga gagctaaaga taaaaagccg ctcgggagta agcgagcggt
     1741 ggtcaaataa ccgacagtac acggagttaa aaaagctctc cgcaatctcg aagcgcgggt
     1801 aatccggcaa cagccgcgtg taatgctctt taacgcgcag taaaaatgcc gcgtccgtac
     1861 tttggccgtt agtaatgcag cgcagttgct ccacgaccag accaacgtga tgatcgtaaa
     1921 gatggatacg gtttttcatc gcctgctgga cagcatgcca gtcggcctgt tcgaaacgct
     1981 gctgcgcccc ggaagtcact tcgaggaatc gaccatactg agcgtcgaag ccttgcaaaa
     2041 tggtttgagc aatcagtaat tccaggccac gcggcattta gcgccctcat caggagcaga
     2101 gaattgccgg atgcggcgta aacgccttat ccagcctacg ttcggcaacg gttgtaggca
     2161 tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg attgccggat gcggcgtaaa
     2221 tgccttatcc ggcctacgtt cggcaacggc tgtaggcatg ataagacgcg caagcgtcgc
     2281 atcaggcatc gtgcaccgat tgccggatgc ggcgtgaacg ccttatccgg cctacagtca
     2341 gcaacggttg ttgttgctta gaactgcgat tcttcagtgg agccggtcag cgcggtgact
     2401 gaagacgtgc cgccctgaat aatagtcgtc actttatcga agtaacctgt acccacttcc
     2461 tgctggtgag atacgaaggt atagccatct ttcgcggcgg caaattccgg ctgctgcact
     2521 ttctcaacgt agtgcttcat accctcgccc tgggcatagg cgtttgccag gtcaaacatg
     2581 ttgaaccaca tgctgtggat acctgccagg gtgatgaact ggaacttgta gcccatatcc
     2641 gacagctgct gctggaagct ggcaatagtt ttgtcgtcga ggtttttctg ccagttgaac
     2701 gacggcgagc agttataagc cagcagtttg cccggatatt tcgcgtggat agcttgtgca
     2761 aagcgacgcg ccagttccag atccggcgtg gaggtttcac accagaccag gtcagcatat
     2821 ggcgcatacg ccaggccacg gctgatcgct tgctcaatgc ccgcatgagt acggaagaag
     2881 ccttcactgg tacgctcgcc ggtaataaat tcgctgtcat acgggtcgca atcggaggtg
     2941 atcagatccg ctgcatcggc atcggtacga gcaaccagta gggttggaac gcccgtcacg
     3001 tcagctgcca gacgcgccgc gaccagtttc tgaatagctt cttgagttgg caccaaaact
     3061 ttgccgccca tgtgaccgca tttcttcact gacgccagct gatcttcgaa gtgaactgcc
     3121 gctgcaccgg cttcaatcat cgctttcatc agttcaaagg cattcaggac accgccaaaa
     3181 ccggcttccg catcggcaac gatcggcagg aagtaatcga catagcgcgg atcgcccggc
     3241 tcaatgcccg cggaccattg gatctgatcg gcacgacgga aggtgttgtt gatccgctcc
     3301 accacagctg gcaccgagtt tgccggatag agcgactgat ccggatacat gctggccgcc
     3361 aggttagcgt ccgccgctac ctgccatccc gacagataga ctgcttcaat acccgctttc
     3421 gcctgttgca gcgcctgacc gccagtcagt gcaccgaggc tgttgatgta gccttttttc
     3481 gactcgccat gcagcagacg ccacattttc gccgcgccca gttgcgccag ggtgcattca
     3541 ggattgactg aaccgcgtaa tttcaccaca tcttccgcgc tgtatgggcg agtaatgcct
     3601 tcccaacgcg gttgagtcca ctctttctgt aattcttcaa tttgttgtgt acgggttttc
     3661 atgtgcagat gctccatagt tatgtggtgg tttacgctaa caggcggtag cctggcaggg
     3721 tcaggaaatc aattaactca tcggaagtgg tgatctgttc catcaagcgt gcggcatcgt
     3781 caaaacgccc ctgggagaaa cgttcttcgc ccagttcgct ggcaatgact ttcatctctt
     3841 cacccagcat ctggcggaac aaggctttgg tcaccggttt gccattgctc aacgtttttt
     3901 gatgatggat ccactgccag attgaggtac gggaaatttc agccgtcgcc gcatcttcca
     3961 tcaggccata aatcggcacg cagccgttgc cggagatcca tgcttcgatg tactgcactg
     4021 ccacgcgaat gttggcgcgc ataccttctt cggtgcgttc accatcacaa ggtgccagca
     4081 gctgatcggc agtaatcggc gcgtcttgtt cgcgcatcac ttcaagctga tttttacggg
     4141 agccgagaat gtcgttgaat accgccattg ccgtatccgc aaggcctggg tgagcaatcc
     4201 atgtgccatc gtgaccgtta ttggcttcca acgctttatc cgcttttact ttgttgagca
     4261 cctggttatt gcgctcttca tctttgctcg gaataaacgc tgccatgccg cccatcgcaa
     4321 aagcaccgcg tttatggcag gttttaatca acaggcgtga gtaagcattc aggaatggtt
     4381 tatccatcgt cactgcctgt ctgtctggca ggacgcgatc gggatagttt ttcaacgttt
     4441 tgatatagct gaagatgtaa tcccaacgac cgcagttcag accaacaata tggtcacgca
     4501 gcgcgtgaag gatttcatcc atctggaaca cggcgggcag cgtttcaatc agcaacgtcg
     4561 ccttgatggt gccgcgcggc agattaaagc gatcttctgc atagctgaag acttcgctcc
     4621 accaggccgc ttcctgccag gactgggttt tcggcagata gaaataggga ccgctgccct
     4681 ttgccaacag tgcctgatag ttgtggaaga aatagagcgc aaaatcaaac aggctaccgg
     4741 ggattgcctc accacgccag gtgacatgtt tttccggcaa gtgcagaccg cgtacccgac
     4801 aaatcaaaac cgctggattg ggcttgagct gataaatttt gcctgcttca ttggtatagc
     4861 tgatggtgcc gttaaccgca tcgcgcaggt taatttgccc gtcgatcact ttgttccagt
     4921 ctggtgccag tgaatcttcg aaatcggcca taaagacttt cacattggcg ttcagtgcgt
     4981 tgatcaccat cttgcgctca accgggccag ttatctcgac tcgacgatct tgtaagtccg
     5041 cgggaatacc acgaattttc cagtccgtat cgcgaatgga agctgtttcc gaaataaaat
     5101 caggcaaagt tccgttatca atatcttgct gctgctgaat gcgcgctgcc agaagtttat
     5161 tgcgttgtgg cgtaaaatgc gtcaccagct cagtcagaaa ttctaccgct tcggcagtaa
     5221 gaatttgctt ctcctgctcg ccatacggcc ttatgaaagc cagttcatcg gttgttgttg
     5281 cctgttcagt catcgtgcag ctcctcgtca tggatccgaa aacttcccca ctgtgaacga
     5341 aggatcgttg tgcgcttttc gttcagcaca actaagacta ctcatttaaa atgcaaaatc
     5401 aaaaacaatt tccattttaa ttaaaaatac ttgataactt attgataaca atatgaataa
     5461 aatttattca cctcctgagg tgcgtttcgg aaataaaaaa ggcacccgaa ggtgcctgag
     5521 gtaaggtgct gaatcgctta acgatcgact atcacagaag attaatccag cgttggattc
     5581 atgtgccgta gatcgtatgg cgtgatctga tagacgtaat agttgagcca gttggtaaac
     5641 agcaaattac cgtgactacg ccagctcgct cgcggtgtat tttgcggatc attgtgcggg
     5701 aaatagttat acggtacttc cgggcctagt ccggcttcca catcgcggaa atattcctgc
     5761 gccagcgttt gcgcatcata ttcgggatgg cccgtcacaa aggcaatgcg cttatctttg
     5821 ctggcaaaca gatatgcatc cccttcttcc gtctctgcca gaatttccag atcggtgtaa
     5881 tcacgaatca acgctgccgg aaagtcagca tagcgcgaat gtggtgccag gaatgaatca
     5941 tcaaagccac gcgtcagaag cgcatgagga tggagaatat gatgctcgta aacgccagag
     6001 agtttgtcgg tgcgagtttg cttaggaatg ccgtagagga tattgagcgc ggcctgtacc
     6061 gcccagcaga caaacagcgt cgaggtgacg tgatctttcg accactccag cacctgtttg
     6121 atctgcggcc agtaagcgac atcattaaac tccaccaggc ccagcggcgc accggttaca
     6181 atcagaccgt caaagttctg ttcctgaata tcttcaaagt tacagtagaa gttgttcaga
     6241 tgctctgcgg gcgtgttgcg cgattcacgg gaatcgatgc gcaacagctg aatatcgacc
     6301 tgcaaaggtg agtttgaaag caggcgcaga aactgatttt cagtttcaat cttcttcggc
     6361 atcaggttaa ggatcagaac cttaagtgga cgaatttcct gaccagacgc acgagaagtt
     6421 gtcatcacaa agacgttttc ttcacgcaag aaattgacgg cgggtagctc gtccggcaca
     6481 cgaataggca taacctgatt acctcactac atacgcttat acgtttagac atccagatag
     6541 ctgaagataa ccagaaaatt tgccaatgtc gagcctgcat gttgaaggtg agaaagtttc
     6601 aaggaaaagg ctacgttaga atataagaat gacgaaaagg agagaaaatg gttattagta
     6661 ttcgccgctc acagcatgag gaaggggagg aactcgttgc gatttggtgt cgatctgtcg
     6721 atgccaccca cgatttttta tcagcagagt atcgggccga gctggaagag ctggttcgtt
     6781 cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga acgggatcag ccggttgggt
     6841 ttatgttgtt aagtgggcag catatggatg cgctgtttat cgatcctgat gtgcgcggct
     6901 gcggcgtggg tcggatgctg gtggagcatg cgctctcgat ggcaccggaa ctgacaacca
     6961 acgttaatga gcaaaatgag caggcggttg ggttctataa gaaggtgggt tttaaggtta
     7021 cgggacgctc tgaggtggac gatttgggga aaccgtatcc gttgctgaat ctggcgtatg
     7081 tgggggaata agccgcccct gccgtgcctg cccaggctgg agaagacgcg ccagcgtcgc
     7141 acccggcatt tttttcacca acgcaaaaag gccatcgtga ctgatggcct tt
//
LOCUS       DAZGXZ010000211         6959 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000211 DAZGXZ010000000
VERSION     DAZGXZ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.211"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..239
                     /locus_tag="ACHKJF_004960"
     CDS             <1..239
                     /locus_tag="ACHKJF_004960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952177.1"
                     /translation="LMSQEITLQQAAERAHQIEVICALAEDYPGMMTDSESGAIIGLL
                     KRLSGEVCVFLSDEQERRTLISNEKKCGGVHHVQ"
     gene            229..450
                     /locus_tag="ACHKJF_004961"
     CDS             229..450
                     /locus_tag="ACHKJF_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952178.1"
                     /translation="MCNNIRPDAAAAALTTLMHALIDIECTAELAQKEEREEYTLFAL
                     ECIRYTATRSLNDAKNILVADCENGGGYA"
     gene            443..664
                     /locus_tag="ACHKJF_004962"
     CDS             443..664
                     /locus_tag="ACHKJF_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952179.1"
                     /translation="MRDDRFNSLKQEFSGVPDDAADALSSISEIMRVAFFFLCTDEHR
                     DTGLNILDIAANYADFVTEVILRKTTDGD"
     gene            666..899
                     /locus_tag="ACHKJF_004963"
     CDS             666..899
                     /locus_tag="ACHKJF_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952180.1"
                     /translation="MRDIYLETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITILEHVIMELQEIDLLHDRYKEQHTGE"
     gene            905..1204
                     /locus_tag="ACHKJF_004964"
     CDS             905..1204
                     /locus_tag="ACHKJF_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0952181.1"
                     /translation="MKLKYSGLTASGKTRTKFMSGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFDRDFSLVQAVPRNVPTSREKARANIQEIKKMLNVFRGKK"
     gene            1201..2607
                     /locus_tag="ACHKJF_004965"
     CDS             1201..2607
                     /locus_tag="ACHKJF_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP0952182.1"
                     /translation="MKLAPNVKLLPKDKHTEAIIFAGNDAHSFAEHYIIAQAKKAGDS
                     TPPVYLGRYQLSELDNLQIVDEGRYRVTVIRAGNIDEAQLLTIATKLAIAGVHEARLL
                     SENFELLEKWTDQLPRLRETWERGESLIMNGGKQKITLPISWGSEGFDAQQSYVIKGL
                     IPAEALCSTYGASGSYKSFLAISWACHVATGMKWGGRRVCKGAVIYVAGEGSMGVKRR
                     VKAWEITHGKAVTDLCIVNSPVFPASPDYVEQVIRTAGLVQDRTGERVRMIVIDTLAR
                     CFGGHDENDSRDMGAFIQGCDAIKQATGATVLVVHHSGKDETKGARGSSAFRAALDAE
                     FRISREHSEATALVAACTKMKDAEEPKESAYDLKSVEVFTDPEGEEIVSMVVIDIPRE
                     PAELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLY
                     KLEKDGVIRIDGDDVAPL"
     gene            2808..3059
                     /locus_tag="ACHKJF_004966"
     CDS             2808..3059
                     /locus_tag="ACHKJF_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952183.1"
                     /translation="MTQKRRDRTEPKYKALDMAELALKVAIRTIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            3056..3466
                     /locus_tag="ACHKJF_004967"
     CDS             3056..3466
                     /locus_tag="ACHKJF_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0952184.1"
                     /translation="MTAQIAAYGRLVDDQQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGYQVIADSVISA
                     RAALPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            3477..3749
                     /locus_tag="ACHKJF_004968"
     CDS             3477..3749
                     /locus_tag="ACHKJF_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0952185.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            3706..3834
                     /locus_tag="ACHKJF_004969"
     CDS             3706..3834
                     /locus_tag="ACHKJF_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0952186.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRFFLHQLKRYGALPGFRHGQA"
     gene            3876..4100
                     /locus_tag="ACHKJF_004970"
     CDS             3876..4100
                     /locus_tag="ACHKJF_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952187.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            4352..4558
                     /locus_tag="ACHKJF_004971"
     CDS             4352..4558
                     /locus_tag="ACHKJF_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952188.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            4558..5613
                     /locus_tag="ACHKJF_004972"
     CDS             4558..5613
                     /locus_tag="ACHKJF_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0952189.1"
                     /translation="MAVKAIALNTHQLFAYLNREDIAEFKFSPLFTALFFPNVATFNT
                     QDIMLDNLDIEEVTMSAFCSPMVGSQVQRDKGYETSIIRPGYMKPKHEIDPLKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQADGKKWSEQDKDIHDPIYDIELYADQAGCPANVMIMGVDVWRMLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQDAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIA"
     gene            5625..5960
                     /locus_tag="ACHKJF_004973"
     CDS             5625..5960
                     /locus_tag="ACHKJF_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0952190.1"
                     /translation="MDTPYIELFAGSQQVATTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            5973..6386
                     /locus_tag="ACHKJF_004974"
     CDS             5973..6386
                     /locus_tag="ACHKJF_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP0952191.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCISVDNAREILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            6590..>6959
                     /locus_tag="ACHKJF_004975"
     CDS             6590..>6959
                     /locus_tag="ACHKJF_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0952192.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IDNRVNNAISQLTNDKGDIDDDELLRVRIRLLTAQAEAQELKNERDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQ"
BASE COUNT         2022 a         1520 c         1924 g         1493 t
ORIGIN      
        1 ggcttatgag ccaggaaatc acactacaac aggcagcaga acgcgcccac caaatcgaag
       61 ttatttgcgc actggcagag gattaccctg gcatgatgac cgacagcgaa tcaggggcaa
      121 tcatcggctt acttaaacgc cttagcggtg aggtctgcgt attcctgagc gatgaacagg
      181 aaagaagaac gcttatttct aacgaaaaaa aatgcggagg ggtacaccat gtgcaataac
      241 atccgtccgg acgcagccgc cgccgcactc actacgctga tgcacgcgct tattgatatt
      301 gaatgcacgg cagagcttgc acaaaaggaa gaacgggagg aatacacatt gttcgccctg
      361 gaatgtatcc gatacaccgc cacgcggtcg ctgaatgacg ctaaaaatat tcttgttgca
      421 gattgtgaaa atgggggggg ttatgcgtga tgatcgtttt aattccctga aacaagaatt
      481 ttccggcgtt cctgatgatg cggctgatgc gctttcgtca atatctgaaa ttatgcgggt
      541 ggcttttttc tttctttgca ctgatgagca cagagataca gggctaaata ttcttgatat
      601 tgccgctaac tatgctgatt tcgtgacaga agttatttta agaaaaacaa cggacgggga
      661 ttaatatgcg tgatatttac ctcgaaacaa tagaccgcgc atttcttgca ctttctcaca
      721 gtgaaaacat gctggaaata ttgcgcatat ggcttgaaac acttggcgac aatgaacgcg
      781 acaaacaaaa atcaagaatt gccacggcat taataacgat tcttgagcat gtaataatgg
      841 aactgcaaga aatagatcta ttgcacgaca gatataaaga acagcacacc ggagaataaa
      901 aataatgaaa cttaaatatt ctggcttaac tgccagtggc aaaactcgca ctaaattcat
      961 gagcggtgat atttaccgcg accagtacgg cggcacggta atgattaagg gcgtggagga
     1021 acggcgcgta acctaccgtc gtgaaggcta cgaatatgat tgcgtgatgc ctgtttatca
     1081 gttcgaccgt gatttttctc tggtgcaggc tgtaccgcgt aacgttccaa ccagcaggga
     1141 gaaagcacgc gccaatattc aggaaataaa aaagatgctt aacgtattca ggggtaaaaa
     1201 atgaaactgg caccgaacgt aaaactatta ccgaaagaca aacacaccga agcaattatt
     1261 ttcgcgggga atgatgcgca ctcatttgca gagcactaca tcattgcaca ggccaaaaag
     1321 gcgggagact ccacgcctcc cgtttacctg gggcgttatc agttaagcga actggacaac
     1381 ctgcagattg ttgatgaggg gcgatacagg gtaacggtga tacgcgccgg aaatattgac
     1441 gaagcgcagc tgttaaccat agcgacaaaa ctggcgatcg ccggagtcca tgaagcgcgg
     1501 ctgctttctg aaaacttcga attactggag aaatggaccg accagcttcc acggctacgg
     1561 gagacatggg agagagggga aagcctgatc atgaacggag gaaaacaaaa aatcacgcta
     1621 ccgatttcat ggggatcgga agggttcgac gcgcagcaaa gctacgtgat taaggggctt
     1681 attccggcgg aagcattatg cagcacctac ggggcgagcg gttcctataa gtcgttcctt
     1741 gccatttcgt gggcgtgtca tgttgccact ggaatgaaat ggggaggccg cagggtgtgt
     1801 aaaggcgcgg ttatctatgt tgctggtgaa ggaagtatgg gcgtaaagcg ccgcgttaag
     1861 gcatgggaaa taacccacgg taaggcggtt actgatttgt gtatcgtcaa ttcgccagta
     1921 ttccccgcat cgccggacta tgtggaacag gttatcagga ccgccggatt agtgcaggac
     1981 agaacgggcg aaagggtgcg gatgattgtc attgacacgc tggcccgttg ctttggtggt
     2041 catgatgaaa atgactcccg cgacatgggg gcgtttatcc aggggtgtga cgcgataaag
     2101 caggccacgg gggccacggt tctggttgtg catcattccg gcaaggatga gacgaaaggg
     2161 gcgcggggtt ccagtgcatt ccgtgcggcg cttgatgctg agttccgaat cagtcgggaa
     2221 cattcagagg ctacggcgct ggttgcagca tgtacgaaga tgaaggacgc ggaggagcca
     2281 aaagaaagtg cctacgacct taagagcgtg gaggtattta cggatcctga gggtgaagaa
     2341 atcgtgtcca tggtggtgat tgacatccca cgcgaaccgg cagaactgga gcgcatcgag
     2401 gaggcaggga acaagacgga aaaccatacc gcgctatggg ggtgcatccg ttcacgcaca
     2461 cagaacggcg acaagtgcac gatcccgctg ttacgtgatg acatgaaaaa actggggtat
     2521 gaaatgaaaa acttccggcg ctggctgtac aagctggaaa aagatggggt tattcgtatc
     2581 gatggggatg atgtagcgcc gctataaaag tgaggagcaa aagcgaggtg gatagaaaga
     2641 gggccaaaat tagcctgctc tccctcactt ttctacctgt atacatcctc aaaagtgagg
     2701 ggtaaaaaaa tacttatgaa acacacacat agaaaaacca aaaatcccaa ttgcgacgaa
     2761 gtgagacgct tgaaaaagtg aggcgaaaaa gtgagaggtt gcgagaaatg acccaaaaac
     2821 gcagagacag aacagagccg aaatataagg cgttagatat ggctgaactg gctttaaagg
     2881 tggcaatcag aacgatagac cgccacgcgg gggaaggata cgcgaaggag catcccgaac
     2941 tgataagcgc attcatgacc acagcagcgg caaactttgc cacgttgaca gaacgggaga
     3001 ttgccgaagc ggaacaggta acaaccatca acgttaaaac cggagagcag acggcatgac
     3061 agcacagata gcggcttacg ggcggctggt ggacgaccag caggtaaaac agaccagcaa
     3121 gggtacaccg atgacgctgg cgcgtatggc ggtatcgctg ccatgtagtc aggcgcagga
     3181 tgggcaggcg acgttatggc tatcggtcat cgcatttggt aagcaggccg acttcctggc
     3241 taaacatcaa aaaggcgacg ttgccagcgt atccggcacg atgcaggtca gccagtggac
     3301 cggacagaat ggggaaacgc ggcagggtta tcaggttatt gcagacagcg taatcagtgc
     3361 ccgtgcggca cttcctggcg ggaacagacg caaaaccaca ggcacacagg gtaatcagcc
     3421 accagcggga ggcgatgacc cttacggtga tgatatttcg ttctgagggg gtgacgatgg
     3481 tacatgaccg catagcggag gaactggagg cgaaaggctt ttaccggagg gcggcggcgc
     3541 gatggggtga agtcatgcag ctggtggaga cagacaagga acggcatcac atcacgatgc
     3601 gacggctgga atgttcaagg aaggcacaga gggcaccgga gccgccggat aatttcggag
     3661 acctgaaaaa ggcagtcgat cgcacttatg ccgaaatggg tatagatggt gctggtgatg
     3721 aaatatggcg caattaccag gacagctaat caaacagccg gagaaatacg gttttttttg
     3781 caccagttga aacggtatgg cgcattaccg ggttttcgtc acggtcaggc atagttacta
     3841 tctgaaacaa acagacacaa cagaggaaaa aaacaatgcc gatgaaattt gatgagatat
     3901 taaaacagcg tgataaatac catgctgaca acatggagac gatgagcatc aatgattacc
     3961 gcgcattcct ggagacgggc gcactgattg aaaaggatca gcatggtttt gtgagatgtg
     4021 ctctatccgg tgaaatgctg gcggtaaatc ctgaacagat agatgcattg atagaatttc
     4081 tgaaagagat cagagactga gccagcacac agcacacata gccggagcaa tccggctttt
     4141 ttgcgcccaa aaaaagcccg ataaggtcag agggttctta tcgggctttt gcatatgagg
     4201 tttttggatg cactggcgtt cgtgatcggg ataatcattt cataatttgc aacataactc
     4261 aatatcattg cataaaatgc aattctgatt ataatcataa ctggataaac atccagttat
     4321 gattttttta gtcgaagagg aatttcttac tatggctgaa gagaaaaaag gcggtgtttc
     4381 ggtgtacata agccccgaaa tcgtggaggt gctcaagcag cgccacaaaa aaaactatga
     4441 agctggcgtt gcggctggac tggatccgct gatgacgccg gagccgtcga taggttcact
     4501 tgtgcgatct tatttacttg cggcgcttgg gatgcacaaa aattatgggg gcgaataatg
     4561 gcagtcaaag caatagcact taacacacac cagcttttcg cgtacctgaa tcgcgaggat
     4621 attgcggaat ttaaatttag tccgctgttt accgcgctgt ttttcccgaa cgtggcgaca
     4681 ttcaacacgc aggacatcat gttagataac ctggatattg aagaagtcac tatgtcggcg
     4741 ttttgttcgc ctatggttgg cagccaggtt cagcgcgata aagggtacga aaccagcatt
     4801 atccgccctg gctacatgaa gccaaaacac gaaatcgatc cattaaaaac aataatgcgc
     4861 atggctggag aagatccggc acagcttaac gatcctacct accgccgtat gcgcctgatt
     4921 actggcaaca tgcgccgcca gataaacgcc attaaggcac gcgttgaatg gctggcggtg
     4981 aatgcggtaa cgaccggaaa aaacatcatt gagggcgaag gcatagaacg ctatgaaatc
     5041 gactggaaga taccggaaaa aaacatcata gagcaggccg acggtaaaaa atggtctgag
     5101 caggataaag acatacacga cccaatatat gacattgaac tatacgcaga tcaggcaggt
     5161 tgccccgcca acgtcatgat tatgggcgtt gatgtatggc gcatgttacg cagctttaaa
     5221 aaattccgtg aactgtacga tctctcccgt ggttcagaat ccgccgcaga gctggcatgt
     5281 aaaaacctgg gcgaagtggt gagctttaaa ggctatcttg gtgatctggc ccttatcgtc
     5341 tattccggca aatacactga cagcgacggc acagaaaaat atttccttga gcctgatttg
     5401 cttgtcctgg gcaacaccaa caataaaggg ctggtggcct atggtgcgat tatggatcag
     5461 gacgcagtaa gaacgggggc aacgcaaaac atgtactacc cgaaaaactg gattgaggac
     5521 ggagatccgg cgattgagta cgtgcagacg cacagcgcac cgcagccggt tccggcagat
     5581 attcgcaaat ttgttactgt caaaattgct taacggggga ttctatggac actccataca
     5641 ttgagttatt tgcaggcagt cagcaggtcg ccacgacgct ggtacatttt gccgctgatg
     5701 ctggcgttat tcaggaattt accccgctga tgctggcgga caatggcgag tttaagccgt
     5761 gggatggtca ggaatctggc aaggctgttt atctgacttc gtatcctgtg gacacgtcaa
     5821 agcagaaatc agcacagtgt tacaagacgg ggatattcaa tattgccgcc gttaactggc
     5881 ctgagagcgt cgacaccgat gcgaaaaaat gcgccgcctt tgcgggttcc ggcgtatccg
     5941 ttcagccgct ggcccgataa gcagggggaa cgatggcaac gaatgaaagc atcatgacgc
     6001 taccgctggc gagtaaattt aaagccgaag cgcgggcaat ggctgacaga ggtttatcaa
     6061 cctatgaggc cgtatatcaa ctaaacaaac tggaagagca ggacaagccg cgcgctgatg
     6121 cgattatggc gcttcatgag cataaagact atcagccgct gttacgtgca atggcaaacg
     6181 tgccatgtat tagcgtcgat aatgctcgtg aaatcctgaa catgaccata gagcaggagc
     6241 gcccaaaggt tgcaccagag cttaccgcag cctttgaaaa ctttatggac atgcacagcc
     6301 cgcaagccgt atcagctggc atggcatacg atggcagaaa ccagggcgat gacggcgaca
     6361 tcgatcgcat actgaaaacc atctgagaca aggccggaga aatccggctt tttttacggg
     6421 tcctttccgg catatggacc cgttacgggg cggcgacctc gcggtttttc gctatttatg
     6481 agccttttca ggggggtggt ggttttgttg tttgctctat ctttatgaat gaaaagggaa
     6541 agatgcaaac aatacaccaa cctgaagcag taattaagtt ggtgtattaa tgaaatcgca
     6601 cctgatgaac aaaaaaaaca tggcgaaaag ctgccgtgta agtgcgacag cgttcgacaa
     6661 gtggggagtg actcccgttg aacgtaaagg ccgtgaggcg ttttacgatg ttgccagcgt
     6721 gatagacaat agggttaaca atgcaattag ccagcttaca aacgacaaag gcgatattga
     6781 tgatgatgaa ctcttacgag tcaggatcag attactgaca gcacaggcgg aggcgcagga
     6841 acttaaaaac gagcgcgatc gcggtgacgt gattgatact gagttttgcc tgtacgcgct
     6901 ttcaaagctg gcgagtcaga tttcatcaat catggacagc ctcccgctta ctatgcaaa
//
LOCUS       DAZGXZ010000212         6926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000212 DAZGXZ010000000
VERSION     DAZGXZ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.212"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..214)
                     /locus_tag="ACHKJF_004976"
     CDS             complement(<1..214)
                     /locus_tag="ACHKJF_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP0952193.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKT"
     gene            complement(211..1881)
                     /gene="menD"
                     /locus_tag="ACHKJF_004977"
     CDS             complement(211..1881)
                     /gene="menD"
                     /locus_tag="ACHKJF_004977"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP0952194.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(1970..3265)
                     /gene="menF"
                     /locus_tag="ACHKJF_004978"
     CDS             complement(1970..3265)
                     /gene="menF"
                     /locus_tag="ACHKJF_004978"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP0952195.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3344..3649)
                     /gene="elaB"
                     /locus_tag="ACHKJF_004979"
     CDS             complement(3344..3649)
                     /gene="elaB"
                     /locus_tag="ACHKJF_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP0952196.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3704..4165)
                     /locus_tag="ACHKJF_004980"
     CDS             complement(3704..4165)
                     /locus_tag="ACHKJF_004980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0952197.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4230..5147
                     /gene="rbn"
                     /locus_tag="ACHKJF_004981"
     CDS             4230..5147
                     /gene="rbn"
                     /locus_tag="ACHKJF_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP0952198.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLVAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5335..6558
                     /gene="elaD"
                     /locus_tag="ACHKJF_004982"
     CDS             5335..6558
                     /gene="elaD"
                     /locus_tag="ACHKJF_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFP0952199.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHAATN
                     SPLKPAGPDNSDNLLFEKLSSPSKLMTIIGDEIPLISEKQSLSKVLLNDKNNELSDGT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYLPDSNSTYLEIALNDNIKIDP
                     SWPKEVQLFPINTGGHWILVSLQKTVNEKNNTQQIKCVIFNSLRALGHDKENSLKRVI
                     NSFNSELMGEMSNNKNITVHLTEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRES
                     NSDSAPYTLLKNYADEFKKYSAEEQYEIDFQHRLINRNCYLDKYGDANINHYYKNLEI
                     KHTQPKNRASGKRVS"
BASE COUNT         1816 a         1789 c         1594 g         1727 t
ORIGIN      
        1 aggttttacg cagtaagtcg gtgacatcat caaatccatc gacgctaatc gccgccgaac
       61 caccatgacc tgggagatca acatacaacc gtgagtagtc ggcaaacgcc tcgcccactt
      121 cttgccattc gtggcaatcg ccggaaaaac cgtgcaaaaa caccagccag ggtaaacctg
      181 gttttccgtg ttttgcctgc gcgtgcagga tcataaatgg cttacctgcg ccagaagttg
      241 ctggagcgtt tgcgcaccat cggtgtcgtt aaccaccatt tcaatcaccg tggtggttgg
      301 cgtgcgccag gcgtcggcaa atgccgtttc aagttcctgc cagttttgcg gacgatgata
      361 tttcagctcg aacatcgcgg cggcgtgctc aaaatggacg ttttgcggca tcagatagaa
      421 acgctcacgc tcgctttgcg gcgttggcaa cagcgagaaa atttgcccgc cgttgttgtt
      481 caccacaatt aataccagcg gcgcagaaac ctgacgcaat aacgccagcg cgttgagatc
      541 gtaaagtgcg gagagatcgc ccacaatcgc cagcgtcggt ttgccgcttg cccgctgaac
      601 gcctgcggcg gtcgaaagca gtccgtcgat accgctggcc cctcggttgc tgtacaccgg
      661 gtaacctgcc ggaagttgcg aaagcgcatc aatcagacgt accaccaggc tgttaccgac
      721 aaacaattgc ccctgttcag gcagatagtc gctgatgcga tgcgccagtt gcgcttcgcc
      781 aaacgcatcg cgacgggcaa taaccgcctg cattgcctgt tccgccaggc gcgggatttc
      841 aacgcaccag ggctggcgtt tttctgccgg atgcagctcc agccagtcgg caatattggc
      901 aattaagcga cgtccgcgat ggtgtgccgg atcaagtcgc ccttcaatgt catcaacaat
      961 ccagtactct tctggttcac agcttgcctg ccattgcagg agccgtttgc ccgtcaggct
     1021 gcttcccagt tgcaccacaa tttgcgcctg ctgcagctcg ctggtcgctt tggcattgcc
     1081 taaccaaaga tcggcacacg gcagcggctg cccggtttgt gacagcacat cgccaatcag
     1141 cggccagcca agagtttgcg cccacagggc aacttttttg ccctcttccg cactcatgcg
     1201 cccggcaacc accacgccgc gcttttgtcg ccagaagaac cagtcgcgct gtttttcact
     1261 ttccagacga ggcgcttcac gcagccacgg tttgtcgtcc tgccaccagt cacccagacg
     1321 ctgttgccag ctaagcccgg tatcgtccat ttcgccatac agcggttcag caaacgggca
     1381 gttgatatgg actccccccg catgaagcgt accgagagcg tggtcgatgg tagaaaccag
     1441 ccaacgtgcg gggatatcct gggtcgggcg cggcaatgaa atactgtgcg tggggtgaga
     1501 ggcgaacatt cccggctggc gaattgcctg attcgcgccg cagtcaatta gctccggcgg
     1561 gcgatcggcg gttaagagaa tcagtttttc tccggttaac ccggcttcaa tcagtgccgg
     1621 atagagattt gccaccgccg tgccggaggt cacaatcacc gccaccggct gcttgctgac
     1681 tttcgccagc cccagcgcca gatgccccaa cccacgctca tcgaaatggg tgtggtgaat
     1741 gaatgcggaa ttctccgccg ccgctaacgt taacggtgta gaacgcgagc ctggggcgat
     1801 acagatgtgt ctgacgccgt gacgcgttaa tgcttccaga atgaccgccg cccagcgtcg
     1861 gttaaatgcg cttactgaca tgagtttgtc cggtatcaat attgcggcta agtataagga
     1921 gctacaaaaa tagattattg atatgaatcg gtaatgatgc gactcattac tattccattt
     1981 gtaataaagt acgcagccct gccgctttgt tgtcgatttc ctgccactct tgctcggggt
     2041 cggaaccacg gacaatgccc gcgccagcat ataatcgcac gacattgccg ctaatttttg
     2101 ctgagcgcag ggaaacgcag aattcgcttt gttgtaatga gagatagccc gcagaaccgg
     2161 cgtaccattc tcgggtgaac ggttcgtgac gggcgataaa ctgtcgcgcc agatcgcgcg
     2221 gtaagccagc aactgctgcc gttggctgca actgatgtaa acagatcaca tcatccgctt
     2281 tgttgagtga agtccagata cagcggcgaa gatgctgcac tttacgcaga cgcagtacct
     2341 gcggcggtaa aacatccagc gtctgggtat cggcctgtaa tcgttggcag atatcttcca
     2401 ccaccagcat gttctcgcgc tggtttttat catccgccat cagccactct cctaactgct
     2461 gcgcctgctt atcatcagga ttatttgcta ctgttcccgc cagcgcttca gtacgcagcg
     2521 ctttgtcacg ccgccgccat aaccgttccg gtgaagagcc aagaaaagca ttttcgccat
     2581 caaaggccat gtaaaaatgg tagcaattca gattcagtcg acgactggca gccatcatcg
     2641 ccgccgcgtt gaccggactt gcgaaatgca ggtcagttgc ccgagcgagc accactttgt
     2701 cgagctcacc ttcggcgatg gttttcgttg ccagttcgat taattgcgtc cagcccgttt
     2761 tgtccggcca gtgttgttct cgcgtggtgg ttaaatgtaa cccaggcaag ggcttgatac
     2821 tcaccagtgt ggcgataaat tcttttgcct gaatcgcatc gtgctgaagg gagctttcgc
     2881 tgaatagcgt cagccgcagc gtggctttac cgccacagcg tcgccattcc aggcggggta
     2941 aaagtaaatt gccctgcgac gggtcaaaag cattcagccc ccaaatgcgt aagtcggcgt
     3001 gttccgggtg ctggcgaaga aaacgttgtg cctggtccaa cgacgtaaaa cgggtaatcg
     3061 cgcccaggac ggcagcttct tcatcaccat tacgttgttg ccagtagaat tgcgggtacg
     3121 tttgctgact ggccagccag ctcaaggcat caaaagcgtc tttgagaggg aaaggaatat
     3181 cgataacccg aatgccgggt gtcgccggaa tctcttgcga caaatggcgc agtagatttt
     3241 ccagcgccgt agtaagtgat tgcacgcgga cctctccctg ttaaaagcct cacattatac
     3301 ggggtactac aaaaaaaatg cagtaccccg gtgtagggag gttttaacgg cgtgccagca
     3361 acagtcctag taccagccca acggccgcgc ccacaccaat tccttgccag ggtttttcgt
     3421 ggacgtagtc atcagcacga taaactgcct gcttcgcccg atagtaataa ctgtctgaag
     3481 cctggctaac ccgttttttc acatcatcca gcgctttttc tgcacgcgct ttcagctcaa
     3541 catatttctg atcggcggga tcgccagagg agcggagcac ctcttccagt gtttcactaa
     3601 gcagcgtcag gtcgtcatcg atacgtgtat caccaaactg attagacatt ctcgttctcc
     3661 atttgcgtaa aacctgtcac taactataga caagggttgt tgattacgcc tgaattacct
     3721 cgcgtgccat cccaatgtgt gggataccat cttcttcata gacctccgtc acagggataa
     3781 aaccgaaact ctggtagaag ttttgcagat gcgcctgcgc cccgaggtaa acaggcttat
     3841 caggccagtg atgcgtacat gtttcgagtg ttttactcat cagctgctgc cccacttttt
     3901 cgccacgcaa cgcttcactg acaatcaccc gacctataac gaccggctca agatcatcat
     3961 cacttttcag aatcctcgca tatgccacca gttcatcatt tttccaaccg agaatatggc
     4021 gattatcccc cgtcaaatca tcaccatcga tatcctgata agggcaattc tgctcgacaa
     4081 caaacaccgc gcaacgcagt tgtaataagg cataaagctg ggaaaccgat aattcagagt
     4141 gatgcagatc ttgccattca atcataatgc tctcctttta gatgtatgcc ttatactagc
     4201 cggaatattt tttgaaacgt gatgaactca tggaattaat ttttttaggt acttcggccg
     4261 gtgtgccaac ccgcacgcgc aatgtcacgg caatattgct taacctgcaa catccgaccc
     4321 agagcggtct ttggttgttt gactgcggtg aaggcaccca gcatcagcta ctgcataccg
     4381 cctttaaccc tggaaaactg gacaagattt ttatcagtca ccttcatggc gatcatcttt
     4441 ttggtttacc cggcttgctg tgcagtcgtt ctatgtcagg cattatccaa cccttaacga
     4501 tttatggtcc gcaaggtatc cgtgaatttg tggaaaccgc gctgcggatt agcggctcat
     4561 ggaccgatta tccgctggaa attgtcgaaa ttggcgctgg cgaaattttc gatgatggcc
     4621 tgcgcaaagt gaccgcttat ccgctggaac acccactgga atgttatggc tatcgtattg
     4681 aagaacatga taaaccgggt gcattaaatg cccaggcatt aaaagctgct ggcgtgccgc
     4741 ctggcccact gtttcaggaa ttaaaagcgg gcaaaacaat cacgttggaa gatggaaggc
     4801 agattaacgg cgcagattac ttagttgctc cagtgccagg taaagcgctc gctattttcg
     4861 gcgataccgg cccctgcgat gccgcacttg acctggctaa aggtgtcgat gtcatggtgc
     4921 acgaagcgac gctggatata accatggaag ccaaagccaa tagtcgcggc catagctcta
     4981 cacgccaggc tgcgacactg gcccgtgagg ctggagtcgg taagctaatc attacccacg
     5041 tcagctcgcg ctatgatgac aaaggttgtc agcacctgtt acgtgaatgc aggtcaattt
     5101 tcccggcgac tgaactggcg aatgatttca ccgtgtttaa cgtttaactc tgcttattat
     5161 taaaaacagg gcgaaacttg ccctgttatc gcaacccgcg ctacatatat ccacccacac
     5221 gggcatattg agcgattttt gatatttatt catctcctca cgcactatat tcagagcaat
     5281 aagttgcctc tatttatctt gattcagcac aacctttgca actaaaagga gcccatgatg
     5341 gttacagttg tcagcaatta ttgtcaatta tctcaaacgc aactcagtca gacatttgca
     5401 gaaaaattta ctgtgaccga ggaattactg cagtctttaa aaaaaacagc gttatccgga
     5461 gatgaagaaa gcattgagtt actgcataat attgcgttag gctatgatga atttggaaaa
     5521 aaagctgaag atattcttta ccatattgtt agaaacccaa caaatgaaac cctatcgatt
     5581 atccgactta tcaaaaatgc ctgtttaaaa ttatataatt tggcacatgc cgcaaccaac
     5641 tcccccctta aaccagctgg tccggataat tcagataatc tcctgttcga aaagctatcc
     5701 tccccgtcaa aattaatgac aattatcggt gatgaaattc ctcttatatc agaaaaacag
     5761 tcgctttcaa aggtgctttt aaatgataag aataatgaac tgagtgatgg tacaaacttc
     5821 tgggataaaa atcgtcaatt aaccacagat gaaatagctt gctatcttca gaagatcgcc
     5881 gctaatgcaa aaaatactca agtcaattat cctactggtc tctacctccc cgattccaac
     5941 agcacttacc tggaaatcgc tctcaatgat aatattaaga tcgatccatc atggccgaaa
     6001 gaagtccagt tattccccat aaatactggc ggacactgga tattagtttc gctacagaaa
     6061 acagttaatg aaaaaaataa cacacaacaa ataaaatgcg tcatattcaa ctcattgcgt
     6121 gcactaggcc atgataaaga aaattcactt aagcgtgtca ttaatagttt taattctgaa
     6181 ctcatgggag aaatgtcgaa taataagaat ataacagttc atttaaccga accagagata
     6241 atatttttac atgccgatct tcagcaatac ttaagccaaa gttgcggtgc atttgtgtgc
     6301 atggcagccc aggaagtgat tgaacaaagg gaaagcaatt ctgacagcgc cccctatacg
     6361 ttattaaaaa actatgctga cgaatttaaa aaatattcag cagaagagca gtacgaaatt
     6421 gattttcaac atcgactgat aaacagaaat tgttatttag ataaatatgg cgatgctaat
     6481 atcaatcatt attataaaaa cttagaaata aaacacacac aacccaaaaa tagagcgtca
     6541 ggaaaaagag taagttaaca tcattgttaa ttaattcgac cagtcagtaa atatgctgac
     6601 tggtaggaca tagtttgcta aaaagtcatc actgactgat gccagtcaca ccatccgcca
     6661 gttcaatctt cgggtatttc cagcgtatct cggcacgcga ttgctggtct gttgatgtag
     6721 tggattgttg ttgctgactt ttcttaccat cagatttctg ccccctgtca actcaatctg
     6781 ccaataaata acataattat atttttcctt atatgaattc ctttttaaaa tagcgaagcc
     6841 actgtaaaag atactttttg tatagttcta acaaccgtaa taaaacaaac gctcataact
     6901 cattataaat aatgataaaa taacgc
//
LOCUS       DAZGXZ010000213         6769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000213 DAZGXZ010000000
VERSION     DAZGXZ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.214"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(175..660)
                     /gene="sixA"
                     /locus_tag="ACHKJF_004983"
     CDS             complement(175..660)
                     /gene="sixA"
                     /locus_tag="ACHKJF_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP0952200.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(863..3007)
                     /gene="fadJ"
                     /locus_tag="ACHKJF_004984"
     CDS             complement(863..3007)
                     /gene="fadJ"
                     /locus_tag="ACHKJF_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP0952201.1"
                     /translation="MEMTSAFTLNVRLDNIAVITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRSSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYDSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3007..4317)
                     /gene="fadI"
                     /locus_tag="ACHKJF_004985"
     CDS             complement(3007..4317)
                     /gene="fadI"
                     /locus_tag="ACHKJF_004985"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP0952202.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQGTAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELHRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4497..4781)
                     /locus_tag="ACHKJF_004986"
     CDS             complement(4497..4781)
                     /locus_tag="ACHKJF_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP0952203.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5153..6493
                     /gene="fadL"
                     /locus_tag="ACHKJF_004987"
     CDS             5153..6493
                     /gene="fadL"
                     /locus_tag="ACHKJF_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP0952204.1"
                     /translation="MSQKTLFTKSALAVAVALISTQVWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1680 a         1830 c         1614 g         1645 t
ORIGIN      
        1 taactttggg caaaatcaag atacgcgcag aagaataatt tccgaatgaa tgtagaaagt
       61 gttttattta tagataagga gagtcttcga tagtagtgag gttgacagat atccgctgcc
      121 tgatgagcaa ttatgcccaa tgaatctacc tcattgggca taatttggaa cacatcagat
      181 agcttttgcc atcttcagat tacacgggct catctgccag ttgaatgtac cgtttccgct
      241 ttcatcaagc gttacgctgg caatggccga ggtggtaaac ataggcggcg tttcgcccgg
      301 acataactcg gcaaccagat acccgactaa cggtaagtga gagatcacca gtaccgaagc
      361 aacaccttca ttggtcaacg cctgcaaata ggcactgacc agaccaacat cgccgcaggg
      421 cgttaactcc ggcagaactt ctgcactgga aggcaggttc agacaatccc ctacttcttc
      481 cagtgtttgc tcggctcgca ggaacgggct caccagaacg cgttcgattt ccactttttg
      541 acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa ccattagtgg tcagaggacg
      601 aaccgaatca ctggcggcat cgagggctgc gtcgccgtga cgcatgataa aaacttgcat
      661 attgcaccgc ttttgttaac cagattcgcc cgttttcttt catctgaaac cagagaaaga
      721 aaacggtggc cggcattgtg ccttatccat tcaccgaatg aaacgctgtt ttttacctca
      781 atggcgtaag tatagtcaat atgcgtttac attttgacca acactccgcc attcagcgcg
      841 gattcatata gctttgacct tcttattgca ggtcagttgc agttgttttc caaaaacttt
      901 ccccacgcgc gcccatctcg accaaacgct cgcaaggggt aaaacgggaa tcatactgcg
      961 tggcaagtcg ttgcattatt gcaaccactt cgcccgcgcc gagagaatcg atatagcgga
     1021 acggtccacc gagaaatggc ggaaaaccaa tgccaaatac cgcgccaata tccccgtcac
     1081 gcacgctacg gataacctgc tcatcaacac aacgtactgc ttcattcagc atcaacatca
     1141 cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg cccttgtgcg ccaatgagcg
     1201 ggtaaatggc aggatcgacc tgttttttgc ttttacgccc tttctgacca taaagataga
     1261 aaccccgacc attttttctg cctttgcgat cgtcgttcaa aattgaagaa acaacatttg
     1321 caggcgcgct aaaacgttct ccataagcgg cttccagtac aggaataatt ttagtcccgg
     1381 tgtcgattcc tacctcatcc aaaagttgga ttgggcctac cggaaaacca aatttcacta
     1441 gcgcagcatc aatgtgctct acccgttcac cttcggtcag catgcggata gcttcattaa
     1501 tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt gtcacgcacg acaatgggcg
     1561 ttttgccctg ttttttcgcc agttttaccg tagtggcgat ggtttgcgct gatgtacccg
     1621 catgaggaat aatctccacc agcggcattt tttccaccgg actgaagaaa tgcaggccga
     1681 taacttgctc aggtcgcgtg gcgtgagcgg cgatatcacc aatcggcaaa gatgacgtat
     1741 tcgaagcaaa gatggtatga gcggcgcaat tttgctccac ttccgccacc atctgttgtt
     1801 ttaattcgag attttcaaac accgcttcaa taatcagatc gcgatgggca aagccgcgat
     1861 agtccgtcgt tccggagatt aatgccagct gtttgtcacg ttcgctggct ttgagatgac
     1921 gacggcgaac tttgccctcc agctgatccc aactgtactt cagcgcatga tttatgccct
     1981 gcgggttgat atctttaatt ctgaccggaa gccccgcttt acaagcagtg acataagcaa
     2041 taccgccgcc catcaagcca ccacctaaaa ttcccacgct gtttaatggc gcaggcggtg
     2101 catcactacc gggatctttc ttcacttccg tactggcaaa aaagatacta cgcaaagctt
     2161 gcgattgtgg cgtcatcgcc agttcgccaa acgcccgagc ttcggcgtca taaccgctgc
     2221 tggtgccctg cgctaatccc gtttcaacaa cctccaggat gcgttctgtc gccggataat
     2281 tgccttgagt tttgtgttct gttttcttgc cgaccatttt gaacagcagc gcacgaccta
     2341 acggccccgc cagaatacgc tcgcgtacag gtagagggcg ggaagatgag cgatccttct
     2401 tcgccagctc aacagcggct tccagcaaaa tggagtgcgg aacaacgtca tccaccagcc
     2461 ccagctttaa tgcctgtttc gcccgaagtt gttttccggt gagaatcatc tctaatgctg
     2521 tactgacgcc tatcagacgc ggtaaacgct gggtgccgcc tgaaccgggt aacaatccaa
     2581 gttgtacttc aggcaaaccg agcaccgttt taggatcgtc agtacaaacg cgaccgtggc
     2641 acgccagcgc cagctccagc ccaccgccca ggcaagcgcc atgaatagcc gcgataaccg
     2701 gaatgggcaa agcatgaatc tccgccatca actgttggcc ctgccgcgcc agtgcttccg
     2761 cttcttgcgc cgttttgcag ttgccgatca tgttgatgtc tgcgccagca atgaagttgt
     2821 ccggtttagc ggagacaaac accacgcctc gcaactcttt gttttcacgg agttgcttaa
     2881 taatggcgcg cacctgcgag gcaaactccg ccttcagggt attcattttc tcacccggta
     2941 cgtcgatggt gataacggca atgttgtcca gacgaacatt aagggtaaac gctgatgtca
     3001 tttccattat tccgcctcca gaaccattgc cgcgccaagc ccaccggcag cacaggcggt
     3061 aactaaacca aatccaccgc cgcgacggtg aagttcatgc aatgtctggg taatcatccg
     3121 cgcgccggtc gccgcgaagg gatgcccgta agcaatcgaa ccgccaagca cgttaaattt
     3181 gctatcgtcc acttcgccag tggcatgtgc acgccccagt acttcacgag caaaacgttc
     3241 gctgcccagc aactgaatat tcgccagtgt ctgagcggca aaggcttcgt gcatatcgat
     3301 caatgtcaga tcgctcatcg tcaaaccggc acgctccagc gccagcggtg ttgaccaggc
     3361 cggaccgagc aacatgtcct gccagacatc aatcgcagta aatgcgtagc tgcgcagata
     3421 ccccagcggc accagcccta attctttcgc tcgggattca gtcatcagga tcaccgctgc
     3481 cgcgccatcg gtcagcggcg tactgtttgc cgccgttacc gttccgtgtt tacgatcaaa
     3541 cgccggacgc agctttgcgt aatcggcaag cgaggaatta ccgcgaatat tgttgtcttc
     3601 gacaagcggt tgtttataag gagggataaa ggcagtcatc acctcttctt tgagttttcc
     3661 gtctgaccat gcctgagcgg cacgctgatg cgaacggtgc gctaatgcat cttgctgttc
     3721 tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg tcgcccatcc gcaagccagt
     3781 agaatattct gctaccgcag gcggtacagg cattaagtcg cgcaaacgca ggcgagagaa
     3841 gagtttcagg cgctggctca tggttcgagc tttgttgaca tcaaccagca cgcgcgccag
     3901 ttttttactg acgccaattg gcaatacgga agaggaatct gccccaccgg caatccccgc
     3961 tcggatagtt cccgccatca ggctttctgc gacgtttgca accgcctgga aactggtagc
     4021 gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc attccggtac cgagaacaat
     4081 ttcacgcgca atgttggggg cttcaggcat ttgtacgacc tgaccaaaga ccagctgttc
     4141 aatcacttca gcggggatct cgctgcgtgc cagcagttcg cctaccacca tcttccctaa
     4201 atcaaccgcg ggaatgccat gaaaagccgt cccctggcgg gcaaaaggcg tacgtaaacc
     4261 gctaacaatg gcgatacgat cgccctggcg ggtaaccagc ggtaaaacct gacccataac
     4321 actcccctgt aaaaaaataa ataaagtggt ctgacctgat catagtctta accatttttt
     4381 tacatttagc caagcggaga aaaaagaacg tgggagctat gacacagtga agacaggaga
     4441 aagaaaagaa aacgcccctg ccagcaactg acaggggcga acgtacccga gaggaattaa
     4501 cgcagaccca gctggaagat cagcatttca gcttcgcagg caaaagtgaa atcgatatcc
     4561 aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga ttttgcacgg ttcggattcc
     4621 acgctacggg ctttttcagt cagcgccgcc agcgtttgtt ctgcttctgc gcggtttgca
     4681 aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca taatggtgcc aacatccata
     4741 cagcagcaaa ccggggtttc atcagcacta catttactca tcgttgattt cctctgtatg
     4801 tgcacccaag gtgccagata aacgttgtgg atattttacg cttccggaaa gtgctgctcc
     4861 agttgttaat tctgcaaaat cggataagtg accgaaatca cacttaaaaa tgatctaaaa
     4921 caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt ttgccagctg gatcgcgttt
     4981 cttagatcat atttgaaaaa agatggaaac atacttgcaa cattccagct ggtccgacct
     5041 atactctcgc cactggtctg atttctaaga tgtacctcag accctacact tcgcgctcct
     5101 gttacagcac gtaacatagt ttgtataaaa ataaatcatt gaggttatgg tcatgagcca
     5161 gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg gcacttatct ccacccaggt
     5221 ctggtcggca ggctttcagt taaacgaatt ttcttcctct ggcctgggcc gggcttattc
     5281 aggggaaggc gcaattgccg atgatgcagg taacgttagc cgtaaccccg cattgattac
     5341 catgtttgac cgcccgacat tttctgcggg tgcggtttat attgacccgg atgtgaatat
     5401 cagcggaacg tctccgtctg gtcgtagcct gaaagccgat aacatcgcgc ctacggcatg
     5461 ggttccgaac atgcactttg ttgcaccgat taacgaccaa tttggttggg gcgcttctat
     5521 tacctctaac tatggtctgg caacagagtt taatgatact tatgcaggcg gctctgtcgg
     5581 gggtacaact gaccttgaaa ccatgaacct gaacttaagc ggtgcgtatc gcttaaataa
     5641 tgcatggagc tttggtcttg gtttcaacgc cgtctacgct cgcgcgaaaa ttgaacgttt
     5701 cgcaggcgat ctggggcagt tggttgctgg tcagattatg caatctcctg ccgggcaaac
     5761 tcctcaaggg caagcattgg cagctaccgc caacggtatc gacagtaata ccaaaatcgc
     5821 tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc ggaatcctgt atgaactgga
     5881 taaaaataac cgctatgcac tgacctaccg ttctgaagtg aaaattgact tcaaaggtaa
     5941 ctacagcagc gatcttaatc gtgcgtttaa taactacggt ttgccaattc ctaccgcgac
     6001 aggtggcgca acgcaatcgg gttatctgac gctgaacctg cctgaaatgt gggaagtgtc
     6061 aggttataac cgtgttgatc cacagtgggc gattcactat agcctggctt acaccagctg
     6121 gagtcagttc cagcagctga aagcgacctc aaccagtggc gacacgctgt tccagaaaca
     6181 tgaaggcttt aaagatgctt accgcatcgc gttgggtacc acttattact acgatgataa
     6241 ctggaccttc cgtaccggta tcgcctttga tgacagccca gttccggctc agaatcgttc
     6301 tatctccatt ccggaccagg accgtttctg gctgagtgca ggtacgactt acgcatttaa
     6361 taaagatgct tcagtcgacg ttggtgtttc ttatatgcac ggtcagagcg tgaaaattaa
     6421 cgaaggccca taccagttcg agtctgaagg taaagcctgg ctgttcggta ctaactttaa
     6481 ctacgcgttc tgataacgcg ttcgcctgga taaagtcacc tgcatagcag gtgactttaa
     6541 ctcccccact tcaccgaaag tagtgtcctc attgcttacc tcctgagttt tgcaaacagc
     6601 ctgttggcag cttgcccttt caaatcaata agcggtatcc acagacaaac ctgaagggaa
     6661 aggcatattt tcaggcgttc tgctcgtcct tctcaaagag tttacttttc tgcatttcca
     6721 ggatactccc ccccctggct attgtgcgct catacactca aattaaaga
//
LOCUS       DAZGXZ010000214         6750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000214 DAZGXZ010000000
VERSION     DAZGXZ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.215"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(179..1108)
                     /gene="fdhE"
                     /locus_tag="ACHKJF_004988"
     CDS             complement(179..1108)
                     /gene="fdhE"
                     /locus_tag="ACHKJF_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP0952205.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1105..1740)
                     /gene="fdoI"
                     /locus_tag="ACHKJF_004989"
     CDS             complement(1105..1740)
                     /gene="fdoI"
                     /locus_tag="ACHKJF_004989"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0952206.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1737..2639)
                     /gene="fdoH"
                     /locus_tag="ACHKJF_004990"
     CDS             complement(1737..2639)
                     /gene="fdoH"
                     /locus_tag="ACHKJF_004990"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP0952207.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2652..5702)
                     /gene="fdnG"
                     /locus_tag="ACHKJF_004991"
     CDS             complement(2652..5702)
                     /gene="fdnG"
                     /locus_tag="ACHKJF_004991"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5115..5117),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0952208.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRNDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5896..6729
                     /gene="fdhD"
                     /locus_tag="ACHKJF_004992"
     CDS             5896..6729
                     /gene="fdhD"
                     /locus_tag="ACHKJF_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP0952209.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1465 a         1834 c         1838 g         1613 t
ORIGIN      
        1 tcttacgctt gtcatcagtg acataccaat aaccttatcg atgtgttgga gggaagaacg
       61 aggcaaacag gcactggcgg catagcttga ggtggtaacg cggaatacgg gagatggtgt
      121 cgtcacaccc tatgggctgt aatccgggcg acctgcgcca cccggaaaac gtcagagatt
      181 actccccttc acccgggaac aggaacgggt tgatggaact gcgggcgtag ccttcttgct
      241 ccattcgcgc gtccagtacc agagaggcga ggtcatctgc cacggcttca actttcggtt
      301 ctttttcctg ataaagaatc ttcaggtaag tgccgcagtc atcgcagctt tcggctttaa
      361 tcgcggcctg ttcgtcatcc agcgaccagt aatgcagttt gccgctctgc tcgcagttgc
      421 tgcattttac gcgcactacg tgccattcgg tttcacacag gttgcagtgc aggtaacgca
      481 gaccctgagt ggtgccaatt tgcaccatgc tggacaccgg catagaacca caaaccgggc
      541 aatattgacg ttgctcgccg tattcagcgc gggctttgcc ggggatcaga ttggccatct
      601 gcgcccagta gagcgacagt gcagcccaga taaacggcgc tttatcgctg ctgacggatg
      661 agaaatcaga ggcaaacagt gcgctggcca tatcttccag ctcctgagtt gatgccttct
      721 ccagattctc aatcactgcc agcgccgggc cgctcatttc aggtttcagc tcagcaatca
      781 gcgccatcag cagcttttgc cagtgcttat cacgcggcag aacgtgaata tccagcgggg
      841 gcttgccctg tgcgctggct tctttaatgc gcgcggtcag atccatctcc agcggatggt
      901 cgtacagcac cacttcctgg gcgtgggcga taagcgcagc aaagcgcagg taatcaccca
      961 gcggattatt ttctgccagc tcgcgcagac gctcggcgcg gcggttgtat aaattcttga
     1021 gccgagggaa caataacggc ggaatcatat ccgccgtacg tttctcgctc gaacccagct
     1081 catcttgcgg gattatgcga atactcattc agctttcttt tccgttgtct tgcggacctc
     1141 acggtaccag cgcgggtgat gtttcttcgc ccaggcgctg gtaacccatc cttccaccat
     1201 ggcggtaatc gtgcctttca cccaaagggc ggcgtagata tgcaccatga taaccacaat
     1261 taacgccact gcggcaaatg aatgcagcat taacgcgaat cggatcaccg ggattgagaa
     1321 agcaggcgca aaataaggac gccagataat cacgccgctc accagcagca gaaccaggaa
     1381 aataatcgcc gcccagaaaa cgcatttctg accgaagtta taacgcccgg tgtcacctac
     1441 ttcctcgttg acgacgatct tacgaatatt cttcgcccaa aagatatcat cccgattgat
     1501 taggttgtga tgccagtaac ggaaaaacat gatgatgaac gaggcaaaca taaccacgcc
     1561 gacaaacggg tgcagaattc gcgccagctg cggtgtgccc atgatttgca tcaaccagtt
     1621 gaaggacggg aacaaaaagc ccagcccgct caccgccgcc aggatgaagc agaaggcggt
     1681 gatccagtgg ttgatacgtt ccggcgcggt gtagcgcacg atggtgtcac gtcgtttcat
     1741 ttgcgctcct cgtctttctc ttcgtgcaga ttattctctt cctcatccgc acggttcgga
     1801 ccgacaccca cgtagtggaa gatactggcc gcaaaggtag ccgcaaagcc aacagccgcg
     1861 agcggtttcc agatgccttt ccagaatttc acggtttcgc tgatttccgg gttctccggc
     1921 aagccatgat acagatttgg cttgtcagca tggtgcagca cgtacatgac gtgtgtacca
     1981 ccgacgcctg ccggatcgta cagacccgca ttgtcgtaac cgcgggtttt cagctcagca
     2041 acgcgctcgc tcgccagcgt tttcatcgac tctttcgtac cgaagtgaat cgcgcccgtt
     2101 gggcaggtct tcacgcaggc tggttcttgc ccaaccacca cgcggtcaac gcacagcgta
     2161 catttgtaga cgcggttgtc ttccgggttg aggcgcggaa tgtcgaacgg acagcccgca
     2221 atgcaataac cgcagccaat gcactgctcg gactggaagt cgacgatacc gttggcatac
     2281 tgaataattg caccttccgc cgggcacgct ttcaggcagc ctggatcgga acagtgcata
     2341 cagccgtctt tgcggatcag ccattccagt ttgtcgttct gctccacttc cgagaagcgc
     2401 atcaccgtcc acgatttggc gcttaaatca ttggggttgt cgtacacccc aatattattg
     2461 ccgacggtat cgcgaatgtc gttccactct gaacacgcca cctgacaggc tttacagccg
     2521 atacaggtgg tgacgtcgat gagtttcgcc acttcttcct ggaagtcccg cgcctgaggc
     2581 gcgggggtca gaccgttagt cgcggaacga cgaatgatat cttgagattg ataagccata
     2641 tgtcgtctcc gttacacctt ttccacattc acaaggaagg acttaaactc cggcgtctgc
     2701 gtgttcgcat caccaacgaa tggcgttaac gtattggcaa taaagccttt tttagctacg
     2761 ccttcataac cccagtgaat cggaataccg atggtatcga tatctttgcc gttcgctttc
     2821 agcgtacgaa tacgtttggt caccaccgct ttggctttga tatagccacg gttggaggag
     2881 actttcacgg tatcgccctg ggcaatgcca agtttattcg ccagcgactc cccgatttcc
     2941 acaaactgct ctggttgcaa aatcgcgttc aacagcgcgt gtttggtcca gtagtggaag
     3001 tgctcggtca gacgataggt ggttccgaca tacgggaact tatcggcttt acccaatgct
     3061 tcggcgtcgt ctttaaagat acgtgcagcc gggttcgaga taacgtttgg atgcagcggg
     3121 ttagttccca gcggcgtttc aaacggctcg tagtgttccg ggaacggacc ttccgccatc
     3181 ttatcgaggg caaacagacg ccccatgcct tcctgctgca tgataaacgg cccgacgccg
     3241 ctacccggag gcgctgcgct gtagtccgga atatcccagc cggtccactt agtgccgtcc
     3301 catttcagca actgacgctt cggatcccac gggttaccct gcggatctgc ggaggcgcgg
     3361 ttatacagaa tgcggcggtt aagcggccat gcccatgccc agcccagcgt gttaccgagg
     3421 ccagacggat cggcgttatc acgacgcgcc atctggttgc cttccggcgt ccagctaccg
     3481 gcgaaaatcc agcagccaca ggaggttgta ccgtcattgc gcaattgggc gaacgagcta
     3541 agttgttggc ctttcttgac gataaccgca ccggttgccg gatcggtaat atcggccagc
     3601 gccttaccgt tgctctccat cgctacttct tccgacttcg gctcatgcgg aatggcgtag
     3661 ttccaggtca tgttcagcac ctggtccggg ttcgcgccac cctgttcggc atacatcttg
     3721 cgcaagcgca ggaagatacc ggagaggatc tcgccatcag tcagcgcaat ccccggggcg
     3781 tccgcacctt tccagtgcca ctgcaaccag cgacctgagt taacgattga accgttctct
     3841 tctgcgaagc aggtcgatgg cagacggaac acttcggtct ggatcttcga cgagtcaact
     3901 tcgttcagct caccgtggtt ctgccagaag ttagaggttt cagtgttcag tgggtcgatg
     3961 gtgacgagga acttcagttt cgacagacaa ccgatcactt tgtttttgtt cgggaatgag
     4021 gcaacagggt taaagccctg gcagatatag ccattgacct tgccctcttt catcatctcg
     4081 aagtactgca ggacgtcgta gcctttatcc cacttcggca accagtcaaa gccccagcta
     4141 ttttccgccg tcgctttatc accaaagaag gccttcatca tagagacgaa gaatttcggg
     4201 tagttgcccc agtagtttac ctggccttcc agcagcggtt ttggcgtgtt ggcggtaagg
     4261 taggtttgca gatcggtctg cttctcgctt ggcagcgtca tgtaacctgg cagactctgc
     4321 gacagtagcc ccaggtccgt cagcccctga atattggagt gaccgcgcag ggcgttaacg
     4381 ccgccgccgg ccatccccat attgccgagc agcagctgga tcatcgccat cgtacgaatg
     4441 ttttgcgcac caacggagtg ttgcgtccag ccgagggcat acaggaacga ggcagtttta
     4501 tcgtgagcac tggtttctgc gatgtattcg cagactttca ggaacgcgtc ttttggcgta
     4561 ccacagatgt tttcaaccac atctggcgtg tagcgggaaa cgtgctgttt cagcaagttc
     4621 cacacgcagc gcggatgttg cagcgtggta tcgcgtttgg cgaagccgtt ttcgtccagt
     4681 tcataagtcc aggaggattt atcgtacttg cgtttttccg cgtcgtagcc ggtgaacagg
     4741 ccatcttcaa agccgtaatc ctcacgcact atcaggctgg cgttggtata ggcttcggtg
     4801 tattcgcggt tgaatttttc attgttcagc aggtacagca atacgcctga caggaaagca
     4861 atgtcagtac cggaacgaat aggggcatag tagtcagcta ccgccgccgt acgcgtaaag
     4921 cgaggatcga tcacaatcag cttcgcgccg ttgtgaattt tggcttccat cgcccagcgg
     4981 aacccgaccg ggtgagcttc agcggcgtta ccgcccatca ccacgacgag gttggcgttc
     5041 ttgatgtcga cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc aagacttgct
     5101 accgttggtc cgtgtcagac acgcgcctgg ttgtcgaccg cgagcatacc cagcgcgcgg
     5161 gagaattttt gcgttaaata gccggtttcg ttactcgacg cggaagcaca cagcatcccg
     5221 gtggagagcc agcggttaac agtcacgcct tcggcgtttt gcgcaatgta gttagcatcg
     5281 cggtcttctt tcatcagttt ggcgatgcga tcaaacgcct cttcccaact gatttgctgc
     5341 catttatcag aacctggcgc acggtattcc ggaaacttca gacggctttc ggagtggatg
     5401 aaatccacca ggccagcgcc tttcggacaa agtgcaccgc ggttgaccgg gtgatccgga
     5461 tcgccttcga tatggaagat agatgctttg gcgttttttg ctccgtcacc gaggctgtac
     5521 atcaacagcc cacagcctac ggaacaatag gtgcaggtat tacgggtttc gcgggtgcgc
     5581 agcagtttat actgccgggt ttccgcgagc gctacgctgg gtgcaaaacc cagtgccgct
     5641 gccgtggtgc ctgccatacc gccagcgcag atcttaaaga actgccttct gctgacctgc
     5701 atggattgct ccttgtttcg acattgtcac gtcccattta cattcgcttg ctgcgtgtgc
     5761 agggagtggg agttattttt ctttgcggaa ggggccgcaa aggtccagaa ttggctcaat
     5821 ttccctccat ccaggaaggg tttgtaacag aataccataa tgttggtgtg tgtgttctta
     5881 tctggttaag agaaagtgaa aaaaacacag caaaaagaaa tcgaaaatgt gacaaatatc
     5941 acaggtgttc gtcaaattga gttatggcgg cgtgatgatt tacaacaccc acggttagat
     6001 gaggtcgcgg aagaagttcc cgttgcgctg gtctacaacg gcatttcgca tgtggtgatg
     6061 atggcgtcgc ccaaagacct tgagtacttt gcgctcggtt tttcgctttc cgaggggata
     6121 atagaaagtc cgcgcgatat cttcggcatg gatgtcgttc cttcctgtaa tggtcttgaa
     6181 gtacaaattg agctttccag ccgccgcttt atggggttga aggagcgccg ccgggcgctg
     6241 gcgggacgta cgggctgtgg cgtatgcggc gtggagcaac ttaatgacat cggaaaaccg
     6301 gtgcagccgc taccgttcac ccagacgttt gatctcaaca aactggatga tgcattacgt
     6361 catctcaacg attttcagcc agtggggcaa ctgactggtt gtactcacgc cgctgcctgg
     6421 atgttgccat ctggcgaact ggtcggcggg catgaagacg tgggtcgcca tgtggcgctg
     6481 gacaaactgt taggccgccg gtcacaagaa ggggaaagct ggcagcaagg tgcggtactg
     6541 gtttccagtc gtgccagtta tgaaatggtg caaaagtcgg cgatgtgcgg cgtagagatt
     6601 ttgtttgcgg tgtctgccgc gaccacgctt gctgtagaag tggctgagcg ttgtaatctg
     6661 acgctggtgg gtttttgtaa gccgggtcgg gcaacggttt atacccatcc gcagcgtttg
     6721 agcaattaat taaaataagt cctgcgatat
//
LOCUS       DAZGXZ010000215         6696 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000215 DAZGXZ010000000
VERSION     DAZGXZ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.216"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(72..1721)
                     /gene="pgi"
                     /locus_tag="ACHKJF_004993"
     CDS             complement(72..1721)
                     /gene="pgi"
                     /locus_tag="ACHKJF_004993"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP0952210.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     regulatory      1958..2154
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2246..3595
                     /gene="lysC"
                     /locus_tag="ACHKJF_004994"
     CDS             2246..3595
                     /gene="lysC"
                     /locus_tag="ACHKJF_004994"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP0952211.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3740..4666
                     /locus_tag="ACHKJF_004995"
     CDS             3740..4666
                     /locus_tag="ACHKJF_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP0952212.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4702..5493)
                     /locus_tag="ACHKJF_004996"
     CDS             complement(4702..5493)
                     /locus_tag="ACHKJF_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP0952213.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5499..6626)
                     /locus_tag="ACHKJF_004997"
     CDS             complement(5499..6626)
                     /locus_tag="ACHKJF_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFP0952214.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILLEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1651 a         1743 c         1604 g         1698 t
ORIGIN      
        1 tcacagacaa ggggatttat ctgataaaac aatgccgaca taacgtcggc attgttatta
       61 aggtttttaa attaatcgcg ccacgcttta tagcggttaa tcagaccatt ggtcgagcta
      121 tcgtggctgc tgatttcttt atcatctttc agctctggca gaatacggtt tgccagttgt
      181 ttacccagct ccacgcccca ctggtcgaag gtgaagatgt tcaggatcac gccctgagta
      241 aagattttgt gctcatacag cgcaatcaac gcccccaggc tgaacggagt gatttcacgc
      301 agcaggatgg agttggtcgg gcggttacct tcgaacactt tgaacggcac cacgtagtca
      361 agcgttgccg gatctttacc ctgatcgcga tattcctgct caaccacttc gcgggattta
      421 ccaaacgcca gcgcttcagt ctgagcaaag aagttagaca gcagtttctg gtggtgatca
      481 gagagcgggt tatgggtgat agccggagcg atgaaatcgc acggtaccat tttggttccc
      541 tggtggatca gctggtagaa cgcgtgctga ccgttagtgc ctggttcacc ccagataatc
      601 gggccagtct ggtaatccac aacgttaccg ttacggtcaa catacttacc gttggactcc
      661 atattgccct gctggaagta cgccgcgaaa cggtgcatat actggtcata cggcagaatc
      721 gcctcagttt ccgcaccaaa gaaattgttg taccagatgc caatcagcgc cagcagtaca
      781 ggcaggtttt tctcggcagg cgtggtggag aaatgcttgt ccatcgcgtg tgcgccagaa
      841 agcagttcaa cgaagttatc aaagccgatg gagagaacaa tcgacaggcc aatcgctgac
      901 cacaaagagt aacggccgcc gacccagtcc cagaactcga acatgttggc agtatcaata
      961 ccaaactcgc caacggcttt ggcattggtt gagagcgccg caaagtgttt cgcaacgtgt
     1021 ttttcatcac ctgccgcttt caggaaccag tcacgcgcgc tatgggcgtt ggtcatggtt
     1081 tcctgagtgg tgaaggtttt agatgctacc aggaacagcg tggtttccgg gtttactttt
     1141 ttcagcactt ccgcgatgtg agtcccatcg acgttagaaa caaagtgcat gttcaggtgg
     1201 tttttgtacg gacgcagtgc ttcggtcacc atgtacgggc cgaggtcaga accgccgatc
     1261 ccgatgttca ctacgtcagt gattgctttg ccggtataac ccttccactc accggagata
     1321 atcgcttccg agaaggtttt catcttctcc agcaccgcgt tgacttccgg cattacgtct
     1381 ttgccatcaa ccaaaatcgg ggtattgcta cggttacgca gcgctacgtg cagcacagcg
     1441 cggttttcag tgcggttgat cttctcgcca gagaacatcg acttaatcgc gcccgccaga
     1501 tcgcactctt tcgccagatc ttgtaatttc gccagcgtct cttcagtgat gcggtttttg
     1561 gagtaatcca ccagcatctg atcgccgaag gttgcggaga acttagaaaa acgatcgccg
     1621 tctttagcaa aaagatcggc gatcgtaacg tctttcattt catcgaagtg tttctgtagt
     1681 gcctgccagg cagcggtctg cgttggattg atgtttttca ttagcaatac tcttctgatt
     1741 ttgagaattg tgactttgga agattgtagc gccagtcaca gaaaaatgtg atggttttag
     1801 tgccgttagc gtaatgttga gtgtaaaccc ttagcgcagt gaagcattta ttagctgaac
     1861 tactgaccgc caggagtgga tgaaaaatcc gcatgacccc gtcgttgaca accgccccgc
     1921 tcacccttta tttataaatg tactacctgc gctagcgcag gccagaagag gcgcgttgcc
     1981 caagtaacgg tgttggagga gccagtcctg tgataacacc tgagggggtg catcgccgag
     2041 gtgattgaac ggctggccac gttcatcatc ggctacaggg gctgaatccc ctgggttgtc
     2101 accagaagcg ttcgcagtcg ggcgtttcgc aagtggtgga gcacttctgg gtgaaaatag
     2161 tagcgaagta tcgctctgcg cccacccgtc ttccgctctt cccttgtgcc aaggctgaaa
     2221 atggatcccc tgacacgagg tagttatgtc tgaaattgtt gtctccaaat ttggcggtac
     2281 cagcgtagct gattttgacg ccatgaaccg cagcgctgat attgtgcttt ctgatgccaa
     2341 cgtgcgttta gtagtcctct cggcttctgc tggtatcact aatctgctgg tcgctttagc
     2401 ggaagggctg gaacctggcg agcgattcga aaaactcgac gctattcgca acatccagtt
     2461 tgccattctg gaacgtctgc gttacccgaa cgttatccgt gaagagattg aacgtctgct
     2521 ggagaacatt actgttctgg cagaagcggc ggcgctggca acgtctccgg cgctgacaga
     2581 tgagctggtc agccacggcg agctgatgtc gaccctgctg tttgtcgaaa tcctgcgcga
     2641 acgcgatgtt caggcacagt ggtttgatgt acgtaaagtg atgcgtacca acgaccgatt
     2701 tggtcgtgca gagccagatg tagccgcgct ggcggaactg gccgcgctgc agctgctccc
     2761 acgcctcaat gacggcttag tgatcaccca gggatttatc ggtagcgaaa ataaaggtcg
     2821 tacaacgacg cttggccgtg gaggcagcga ttatacggca gccttgctgg cggaggcttt
     2881 acacgcatct cgtgttgata tctggaccga cgtcccgggc atctacacca ccgatccacg
     2941 cgtagtttcc gcagcaaaac gcattgatga aatcgcgttt gccgaagcgg cagagatggc
     3001 aacttttggt gcaaaagtac tgcatccggc aacgttgcta cccgcagtac gcagcgatat
     3061 cccggtcttt gtcggctcca gcaaagaccc acgcgcaggt ggtacgctgg tgtgcaataa
     3121 aactgaaaat ccgccgctgt tccgcgcgct ggcgcttcgt cgcaatcaga ctctgctcac
     3181 tttgcacagc ctgaatatgc tgcattctcg cggcttcctc gcggaagttt tcggcatcct
     3241 cgcgcggcat aatatttcgg tagacttaat caccacgtca gaagtgagcg tggcattaac
     3301 ccttgatacc accggttcaa cctccactgg cgatacgttg ctgacgcaat ctctgctgat
     3361 ggagctttcc gcactgtgcc gggtggaagt agaagaaggt ctggcgctgg tcgcgttgat
     3421 tggcaatgac ctgtcaaaag cctgcggcgt tggcaaagag gtattcggcg tactggaacc
     3481 gttcaacatt cgcatgattt gttacggcgc atccagccat aacctgtgct tcctggtgcc
     3541 cggcgaagat gccgagcagg tggtgcagaa gctgcatttt aatttgtttg agtaatattt
     3601 ccctactggc gtataacgct caccgattgt tatacgccat cctcactgaa aacatagatt
     3661 aaataatatc tgaaaagcaa aatcactcct gtagaacagt attataatca ccgaaaaata
     3721 tcatttaaca ggagtgttaa tggctacttt aatggaaaaa gatgcacttt taaacggagc
     3781 cagtcagtgc atcgcgtttt tatcaaatat cattgataat tgctcagttt cgtcgcatca
     3841 ggattcaggc gatgctctca agcgattagt cagctatcgc gattatcttt attctacccc
     3901 cgctgaactt gttgatttta cacaaggtaa gattctcctt cagcaggtaa gaactcaata
     3961 tcagcatgaa ttcaacaata ctacgcattc agaaaataaa gcatcttttg attctatttg
     4021 gcagcgtctg acaaatcatg aagtcacacc gcaacagcat cccattggtt ttgttttagg
     4081 tgggcaaccc ggagcaggta aatcatctct gattgaatta gccaaaagag agacaaaaga
     4141 taatataatg attattaacg gtgatgattt tcgatttcta caccctgatt ttaattatat
     4201 ctaccaaaat tatggggatg attttgttac ccatacagcg aaattttccg gcgaaacggt
     4261 cgaacgtgca attgagagag cgatagtcag taaactcaac attgttgtcg aaggaacatt
     4321 tcgtaatgcc gcgacgccgt tacaaacatt aaaaaaactc aaagatgcag gctaccaaac
     4381 agaagtcatg ataaaaacaa cctcagcggc attaagctgg gaaagcacca atgaacggta
     4441 tagtaaggac aaagaggcgg gaaatattgc ccgaaaagtg gataaaaacc accatgatat
     4501 cgtcaccggt ttgctggcag aaaatgcccg caaggtgttt gcttcgaacc tgtcagataa
     4561 gtttgcggtg tattcgcgtg agaagatgat atttagttca caagcggcaa cgaatgacga
     4621 tattgcaaca cttatacaga atgaaatcag cgggaataca caataataat aagagctgat
     4681 ggcgtgaaat aacgccgggc tttatgcccg gcgcattacc gtctggtaca ttaagctatc
     4741 cagttcctta acgtcccctt ctctgctatc cggtaacagc ggtggaattt ccccacgcgc
     4801 cagttccgca cgaataaacg catgtaacaa tggacggcgc ggaccatatt ccgcttctcc
     4861 tgcgcgctgt ttacgttcca gtaattcgtc tatttctgca cgtaacggcg catcaagttc
     4921 gcttcccgcc agtaactcag caaagcgcat tggcggtacg cctttacccg cttcgaccca
     4981 gcgcacggcc agcagcggac gcagcacgta aaagtatttt ttcagtcgaa cttcgtcgcc
     5041 ctgcaaatat ccggggaagt ttttctgcgc catcgaatag taatgccagc gcgcacgcag
     5101 cggagaaaac cacattggga ccattgcctt aaacgccgtt atcgtttctt catcttgttg
     5161 ataaatcacc ggagaatcca gccattctat cagcgtcggg ttcgcgcctt tcaataatcc
     5221 cagcgcctta cgccactccc aaccggaaac atctaactcg tcgtcgatgg gcagctcaat
     5281 aacatcgcgc ggtgactcta ctcgcagata ccattccagc gggtgaacat acaaaaaccg
     5341 tacatcgtag tcgctgtcgg gcgaggcaaa tccccagccg cggctccctg attcacaggc
     5401 gtacagtact ttcacatcat accgccgttc aatttctttt aactgctgcg ataccctttc
     5461 gcgcatcgca gcactgactc catttaatgg catgttactc accctttgat attaaccaac
     5521 ccatgcagaa cctgatccag accgccaaat acagagatgt tatcgatccg ctcagccact
     5581 ctctccagcg tttctagctc ttttaaacgc aacgctaccg gattgttctc catgactttg
     5641 gcggtgttca gtaacgagcg cgtcgctgcc gtttcttcac gtcggcgaat cacattcgcc
     5701 tgcgcagatt tctctgcctc aaccagctgc gcgaggatat ttttcatatc accaggcagg
     5761 acgatatctt tcacccctaa tgaggcaatc tccataccaa atggcaacat gcgcgatttc
     5821 acctgttcgc tgacaacgtc atcaatgacc tgtttatctt ccagcagttc atccagcgta
     5881 cgagtaccta ccgcttcacg cagggcaaat tgcaattccc gatacaaatg atcaatcggc
     5941 ttcgttaact gactaaatgc cagcaacacg tcgctgtagc gccagttagc ggcgaggtta
     6001 atccgcagat tgaccttatc tttcgttaag atttcttgcc cgctgacttc cagtacctgc
     6061 aagcgggtgt caaccacttc agcgtcaacc agatggttaa ttttccagta tgcggtcagc
     6121 cccggcggta acagcgcctg cgtttcacca tcgattttca gcacgcctac atgccaggcc
     6181 ggaacctgca ccgttagtat tgcatcgcgg cctttaactg cccctttgcg ccgtggttgc
     6241 aataccgcat tcatcacctc cgtttgtacc tggacttgct gcgtattcat ccgcaccagt
     6301 gtcaaatcat cttctacacg ccagtacaaa cggcgcgttg atggcggcag aatctccagc
     6361 aaaataccgt ccatatacaa cgccccggct tcaatctccg acaagtctgc caccaggcaa
     6421 tatttctcca cccaatcagg ctgaaagcgg cgcaaataat ccgccagcac gtcgggaact
     6481 tcgctgccat cgagcgtaat cagcaagact tctggcgtat ttaaccaggg taaaagatgc
     6541 tcgcctgcat gtagcacttt gtagtaatca ccgtttctgg agagtaatgc cagctgatct
     6601 tttcttacgc ttattttttt aatcattatc tattccttgc ttaacagccg tcgcggaagc
     6661 agacacgaag cgacagggga attccctttt gcctgt
//
LOCUS       DAZGXZ010000216         6641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000216 DAZGXZ010000000
VERSION     DAZGXZ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.217"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            66..1172
                     /locus_tag="ACHKJF_004998"
                     /pseudo
     CDS             66..1172
                     /locus_tag="ACHKJF_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            1508..2485
                     /gene="glaH"
                     /locus_tag="ACHKJF_004999"
     CDS             1508..2485
                     /gene="glaH"
                     /locus_tag="ACHKJF_004999"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP0952215.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            2505..3773
                     /gene="lhgO"
                     /locus_tag="ACHKJF_005000"
     CDS             2505..3773
                     /gene="lhgO"
                     /locus_tag="ACHKJF_005000"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP0952216.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAMELREREPNITGLGGIFVPSSGIVSYRNVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            3796..5244
                     /gene="gabD"
                     /locus_tag="ACHKJF_005001"
     CDS             3796..5244
                     /gene="gabD"
                     /locus_tag="ACHKJF_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0952217.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLENGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5258..6538
                     /gene="gabT"
                     /locus_tag="ACHKJF_005002"
     CDS             5258..6538
                     /gene="gabT"
                     /locus_tag="ACHKJF_005002"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP0952218.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
BASE COUNT         1659 a         1681 c         1789 g         1512 t
ORIGIN      
        1 tgagtcacct ctgactgaga gtttactcac ttagccgcgt gtccactatt gctgggtaag
       61 atcaagataa catctctaag gatgaatggg aaaacctcca tgcattaaaa gaaaagcgtt
      121 taaatggaat gcctaaaaca acacccaaaa gtgaccaggt tattatgttg caaaatcaat
      181 acgttcgtga aatgcgaaaa tatggcgtac gaggtttacg ttatgatgcg gcaaaacact
      241 caaaacatga acaaatagag aggtcgataa ccccaccgct taaaaattat aatgagcggt
      301 tacacaatac taatttattt aacccaaaat atcaaaaaaa agccgttatg aattacatgg
      361 aatacctggt aacttgtcag ttggatgaac aacaaatgtc atcactgctt tatgagagag
      421 atgatttaag tgccattgat ttttctttac tcatgaaaac gataaaagcc ttttcatttg
      481 gtggagatct ccaaaccctt gcatcaaaac cgggttccac aatctcaagt atcccatcag
      541 aaagacggat attgattaac attaaccacg attttcctaa caatggtaat cttttcaatg
      601 actttctatt taaccatcaa caagatgaac aattagcaat ggcatatatg gccgctctcc
      661 cgttcagcag acctttagtt tacagggatg gccaggtatt aaaatcaacg actgaaatta
      721 aaaattatga tgggtccacg cgtgtcggcg gtgaggcgtg gcttaataaa ggttgctcta
      781 cctatcagca gctctacaat gaattccacg cattatatat agataaagca ggaatatgga
      841 gcgcatttga gggtgtattt gcaactaaaa acgttctggc ctttagtcgt ggggattctg
      901 tgaacattaa tcactctcct catgatggac tagttataat aaataaagga aacgaagaag
      961 ttgaaggtac ctggcctaac aaattgcaac ctggaatata caaaaacatg gggagtaata
     1021 gcgttaacat tattattaat aatacccgaa aaattatccc ccctggtaaa gtatttacgc
     1081 ttagaggcgg aactctaaat atcaatattc ctgggcgtag cgctcttctt ttagggaaaa
     1141 caggagaacc gccgaactat ctctatttat aatttaatgt tatatctgcc ccgataaaac
     1201 ggggcagata atatgtttag tttactaacg gtcattttgc agtgaagcca tttactgttt
     1261 tttatcgacc agataatctg ttctctaatg ttaactcccc ctaacctgtt gctttagtta
     1321 ttcatttcct gtctcacttt gccttaatac cctacgttaa atgttactaa tttattgctt
     1381 ttgatcacaa taagaaaaca atatgtcgct tttgtgcgca tttttcagaa atgtagatat
     1441 ttttagatta tggctacgaa atgagcatcg ccatgtcacc ctacatctca taagaggatc
     1501 gcttctgatg aatgcactga ccgccgtaca aaataacgct gtcgattcag gccaggacta
     1561 tagcggattc actctcatcc cgtcggcgca atccccgcgt ctgctggaac tcaccttcac
     1621 cgaacagacg accaaacagt ttctcgaaca ggttgccgaa tggcccgtgc aggcgctgga
     1681 gtacaaatcg tttctgcgtt ttcgggtagg caaaattctt gacgatctgt gtgcgaatca
     1741 gctgcaacca ttgctgttga agaccctgct aaaccgcgct gaaggtgcgc tgttgatcaa
     1801 tgcggtgggt atcgatgatg tggcgcaggc agatgagatg gtgaagctgg caacggcggt
     1861 agcgcatctg attggtcgct caaatttcga tgcgatgagc ggtcagtatt acgcgcgttt
     1921 cgtggtgaaa aatgtcgata actcagacag ctatctgcgt cagccgcacc gcgtgatgga
     1981 actgcacaac gacggcacgt atgtcgaaga gatcaccgat tacgtgctga tgatgaaaat
     2041 cgatgagcaa aacatgcagg gtggaaattc gttgctgctg catctcgatg actgggaaca
     2101 tctggaccac tatttccgcc atccactggc gcgtcgcccg atgcgctttg ccgcgccgcc
     2161 gagcaaaaac gtcagcaaag atgttttcca tccagtgttc gacgttgatc aacagggtcg
     2221 cccggtgatg cgctatatcg accagttcgt ccagccaaaa gattttgaag aaggcgtgtg
     2281 gttgagcgag ctttccgatg ccattgaaac cagcaaaggc attctttctg tacccgttcc
     2341 cgttggcaaa ttcctgttga taaacaacct gttctggctg cacggtcgcg accgctttac
     2401 tccgcacccg gatctgcgcc gtgaactgat gcgtcagcgt ggctatttcg cttacgccac
     2461 tcaccactac cagacgcatc agtaagcgca aaggaattga gcggatgtat gattttgtga
     2521 ttattggcgg cggcatcatc ggcatgtcga ccgccatgca actgattgat gtttatccgg
     2581 atgcccgcat tgcgttgctg gaaaaagagt ccggcccggc ctgtcaccag acgggccaca
     2641 acagcggcgt gatccatgcc ggggtttatt acacgcccgg tagcctgaag gcgcagtttt
     2701 gcctggcggg aaaccgcgcc accaaagcct tttgcgatca aaacggcatt cgctatgaca
     2761 actgcggcaa gatgctggtc gccacctctg aactcgaaat ggaacggatg cgcgcgttgt
     2821 gggaacgcac ggcggcaaac ggtatcgagc gcgagtggtt aaacgccatg gaactgcgcg
     2881 agcgcgaacc gaatatcacc gggcttggcg gcatttttgt gccatccagc ggcattgtca
     2941 gctaccgcaa cgtgacggcg gcaatggcaa aaatcttcca ggccagaggc ggtgagatta
     3001 tctataacgc cgaagtcagc ggcctcagtg agcataaaaa cggcgtggtg atacgtaccc
     3061 gccagggcgg cgaatatgaa gcatcgacgc tgattagctg ttccgggctg atggctgacc
     3121 ggctggtgaa aatgctcggc ctcgaaccgg gctttattat ctgcccgttc cgcggcgagt
     3181 atttccgcct tgcgccggag cataaccaga ttgttaacca tctgatttac cctattcccg
     3241 acccggcaat gccgtttttg ggcgtgcatc tcacccgaat gatcgatggc agcgtgaccg
     3301 tcgggccaaa cgcggtgctg gcttttaaac gcgaaggcta ccgcaagcgc gacttctcgt
     3361 ttagcgacac gctggaaatt ttaggctcgt cggggattcg ccgggtgctg caaaaccatc
     3421 tacgctcagg actgggcgag atgaaaaact cgctgtgcaa aagcggctat ctgcggctgg
     3481 tgcaaaagta ttgtccccgg ctttcgttaa gcgatctcca gccctggccc gccggtgtgc
     3541 gggcgcaggc ggtatcgccg gatggcaagt tgattgacga ttttctgttt gtcaccaccc
     3601 cgcgcacgat ccacacctgc aatgcgccct ccccggcagc gacatcagca attcctattg
     3661 gtgcgcatat tgtcagcaag gtacaaacgc tgttggcaag ccagagtaac cccggacgca
     3721 cgctgcgagc ggcacgtagt gtggatgcct tacacgccgc atttaatcaa taacctttga
     3781 aaacaggacg taacaatgaa acttaacgac agtaacttat tccgccagca ggcgttgatt
     3841 aacggggaat ggctggacgc caacaatggt gaagtcatcg acgtcaccaa tccggcgaac
     3901 ggcgacaagc tgggtagcgt gccgaaaatg ggcgctgatg aaacccgcgc cgctatcgac
     3961 gccgccaacc gcgccctgcc cgcctggcgt gcgctcaccg ccaaagaacg cgccaacatt
     4021 ctgcgcaact ggttcaattt gatgatggag catcaggacg atttagcgcg cctgatgacc
     4081 ctagaacagg gcaaaccgct ggctgaagcg aaaggcgaaa ttagctacgc cgcctccttt
     4141 attgagtggt ttgctgaaga aggcaaacgc atttatggcg acaccattcc cggtcatcag
     4201 gccgataagc gcctgattgt tatcaagcag ccgattggcg ttaccgcagc tatcacgccg
     4261 tggaacttcc cggcggcgat gattacccgc aaagccggtc cggcgctggc ggcaggctgc
     4321 accatggtgc tgaaacccgc cagtcagacg ccgttctctg cgctggcgct ggcggagctg
     4381 gcgattcgcg cgggcattcc ggctggggta tttaacgtgg tcaccggttc ggcgggcgca
     4441 gtcggtaacg aactgaccag caacccgctg gtgcgcaaac tgtcgtttac cggttcgacc
     4501 gaaattggcc gccagttaat ggaacagtgc gcaaaagaca tcaaaaaagt gtcgctggag
     4561 ctgggcggca acgcgccgtt tatcgtcttt gacgatgccg atctcgataa agccgtggaa
     4621 ggtgcgctgt cctcgaaatt ccgcaacgcc gggcaaacct gcgtctgcgc caaccgttta
     4681 tacgtgcagg acggcgtgta tgaccgcttt gccgaaaaat tacagcaggc ggtgagcaaa
     4741 ctgcacatcg gcgacgggct ggagaatggc gtcaccatcg ggccgctgat cgatgaaaaa
     4801 gcggtagcaa aagtggaaga gcatattgcc gatgcgctgg agaaaggcgc gcgcgtggtt
     4861 tgcggaggta aagcgcacga acgcggcggc aacttcttcc agccaaccat tctggtggac
     4921 gttccggcca acgccaaagt gtcgaaagaa gagacgttcg gccccctcgc cccgctgttc
     4981 cgctttaaag atgaagccga tgtgattgcg caagccaatg acaccgagtt tggccttgcc
     5041 gcctatttct acgcccgtga tttaagccgc gtcttccgcg tgggcgaagc gctggagtac
     5101 ggcatcgtcg gcatcaatac cggcattatt tccaatgaag tggccccgtt cggcggcatc
     5161 aaagcctcgg gtctgggtcg tgaaggttcg aagtatggca tcgaagatta cttagaaatc
     5221 aaatatatgt gcatcggtct ttaactggag aacgcgaatg agcagcaata aagagttaat
     5281 gcagcgccgc agtcaggcga ttccccgtgg cgttgggcaa attcacccca ttttcgctga
     5341 ccgcgcggaa aactgccggg tgtgggacgt tgaaggccgt gagtatcttg atttcgcggg
     5401 cgggattgcg gtgctcaata ccgggcatct gcatccgaaa gtggtggctg cggtggaagc
     5461 gcagttgaaa aaactgtcgc acacctgctt ccaggtgctg gcctacgagc cgtatctgga
     5521 gctgtgcgag attatgaatc agaaggtgcc aggcaatttt gccaagaaaa cgctgctggt
     5581 caccacaggt tctgaagcgg tggaaaacgc ggtgaaaatc gcccgcgccg ccaccaaacg
     5641 tagcggcacc atcgctttta gcggcgcgta tcacgggcgc acgcattaca cgctggcgct
     5701 gaccggcaag gtgaatccgt actctgcggg catggggctg atgccgggtc atgtttatcg
     5761 cgcgctttat ccttgcccgc tgcacggcat aagcgaggat gacgctatcg ccagcatcca
     5821 ccggatcttc aaaaatgatg ccgcgccgga agatatcgcc gccatcgtga ttgagccggt
     5881 tcagggcgaa ggcggtttct acgccgcgtc gccagccttt atgcagcgtt tacgcgctct
     5941 gtgtgacgag cacgggatca tgctgattgc cgatgaagtg cagagcggcg cggggcgtac
     6001 cggcacgctg tttgcgatgg agcagatggg cgttgcgccg gatcttacca cctttgcgaa
     6061 atcgatcgcg ggcggcttcc cgctggcggg cgtcaccggg cgcgcggaag tgatggatgc
     6121 cgtcgctcca ggcggtctgg gcggcaccta tgcgggtaac ccgattgcct gcgtggctgc
     6181 gctggaagtg ttgaaggtgt ttgagcagga aaatctgctg caaaaagcca acgatctggg
     6241 gcagaagttg aaagacggat tgctggcgat agccgaaaaa cacccggaga tcggcgacgt
     6301 acgcgggctg ggggcgatga tcgccattga gctgtttgaa gacggcgatc acaacaagcc
     6361 ggacgccaaa ctcaccgccg agatcgtggc acgcgcccgc gataaaggcc tgattcttct
     6421 ctcctgcggc ccgtattaca acgtgctgcg catccttgta ccgctcacca ttgaagacgc
     6481 tcagatccgt cagggtctgg agatcatcag ccagtgtttt gctgaggcga agcagtagct
     6541 atgtccgttt gtaagagtta aacgttgcta agggaatgat aacgatgcag taaaaaataa
     6601 taacgatgag gcgatcgaca aagttaaaaa cgactgcgga c
//
LOCUS       DAZGXZ010000217         6621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000217 DAZGXZ010000000
VERSION     DAZGXZ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.218"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..895
                     /locus_tag="ACHKJF_005003"
     CDS             <1..895
                     /locus_tag="ACHKJF_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0952219.1"
                     /translation="IEQAVEELGYIPNRTAGALASGHSDTVAVLVPSLTDKTSSRFMQ
                     LLQQVLNKNEFQLLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKT
                     NIPTINVVGSPFSPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQL
                     NGWHKAMLSYYQNSDLVITMPGAASLQLGRYALNEILLRQPELDAVICSHEEIALGIM
                     FECQRRLLKIPGNIAVACLDGSDSCDQTHPTLTSIRIDYKKMGTETGKLLIGLLNNNH
                     DESEESRIVQFNYQIELRQST"
     gene            1098..1778
                     /locus_tag="ACHKJF_005004"
                     /pseudo
     CDS             1098..1778
                     /locus_tag="ACHKJF_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1795..2103
                     /locus_tag="ACHKJF_005005"
                     /pseudo
     CDS             1795..2103
                     /locus_tag="ACHKJF_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(2206..3312)
                     /locus_tag="ACHKJF_005006"
                     /pseudo
     CDS             complement(2206..3312)
                     /locus_tag="ACHKJF_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            4078..6126
                     /gene="ireA"
                     /locus_tag="ACHKJF_005007"
     CDS             4078..6126
                     /gene="ireA"
                     /locus_tag="ACHKJF_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor IreA"
                     /protein_id="HFP0952220.1"
                     /translation="MKNKYIIAPGIAVICSAVISSGYASSDKKEDTLVVTASGFTQQL
                     RNAPASVSVITSEQLQKKPVSDLVDAVKDVEGISITGGNEKPDISIRGLSGDYTLILV
                     DGRRQSGRESRPNGSGGFEAGFIPPVEAIERIEVIRGPMSSLYGSDAIGGVINIITKP
                     VNNQTWDGVLGLGGIIQEHGKFGNSTTNDFYLSGPLVKDKLGLQLYGGMNYRKEDSIS
                     QGTPAKDNKNITATLQFTPTESQKFVFEYGKNNQEHTLTPGDSLDAWTMRGNLKQPNS
                     KRETHNSRSHWVAAWNAQGEILHPEIAVYQEKVIREVKSGKKDKYNHWDLNYESRKPE
                     ITNTIIDAKVTAFLPENVLTIGGQFQHAELRDDSATGKKTTETQSVSVKQKAVFIENE
                     YAATDSLALTGGLRLDNHEIYGSYWNPRLYAVYNLTDNLTLKGGIAKAFRAPSIREVS
                     PGFGTLTQGGASIMYGNRDLKPETSVTEEIGIIYNNDSGFSASATLFNTDFKNKLTSY
                     DIGTKDPVTGLNTFIYDNVGEANIRGVELATQIPVYDKWRVSANYTFTDSRRESDDES
                     LNGKSLKGEPLERTPRHAANAKLEWDYTQDITFYSSLNYTGKQIWAAQRNGAKVPRVR
                     NGFTSMDIGMNYQFLPGTLINFAVLNVTDRKSEDIDTIDGNWQVDEGRRYWANVRISF
                     "
BASE COUNT         1879 a         1443 c         1500 g         1799 t
ORIGIN      
        1 gatagaacag gccgtagaag agttgggtta tattcccaac agaactgctg gcgcccttgc
       61 atctggtcac agtgatacag tcgccgtact ggttccttcc ctgacagata aaaccagctc
      121 ccgctttatg cagttgttgc agcaggtgct taataaaaat gaattccagc tactgcttgg
      181 gtgtcatgag tataatcagc tcaaagaagc tgaaatcctg atgacgcttc ttcaaggtaa
      241 cccggcggcg ttagttattt ttggttctca gttggctgat aaaactcacc agatactcga
      301 gaaaacaaat atacctacca ttaacgttgt cggttcccct ttcagtccgg ctcaaattac
      361 catcgaaaca gcattttttg aagcatcaca taaactgacc gaacatctgc tggaacaggg
      421 ttataagaat atcggattta tcggtgcgca tatggacaac cgcctgcaac gtcagcaact
      481 gaacggctgg cataaagcaa tgctgagcta ttaccagaac tctgatcttg ttatcaccat
      541 gccgggtgcc gcaagtttgc aattggggcg ttacgcatta aacgagatat tactgcgaca
      601 accagagctg gatgctgtga tttgcagcca cgaagagatt gcgctgggta tcatgtttga
      661 gtgccagcgg cgtttgttaa aaatccctgg aaatatcgct gtagcttgtc ttgacggttc
      721 agacagttgc gatcaaacac acccaacctt gacatccatc cgtattgact acaaaaaaat
      781 ggggacagag acgggtaagc tgctgattgg tctattgaat aataatcatg atgaatcaga
      841 agagtcgcgt atcgtgcaat tcaactacca gatagaatta cgtcagagta catgagctca
      901 aaaggggatt attgcaaagt tagctattgt ggtagggata gccgtttccg gctattcccc
      961 gcgcagagcc ggatgtgtgc tgctaacgca tcctgtcaac gacggatgaa aagttatcca
     1021 cttatatctc caccaacggc ccaatattgt atcgttcgtg acacatccat ttctacttca
     1081 ggtatttggc gtagtagcgg gtgctaaaaa tttgccgcaa aaccgcaata tcagcccgca
     1141 gaggcgctct ccagattatc cattcctata ttatctctgc accggatgaa ttactcgaac
     1201 aactcagaaa tatgacacat atgcaactta tcaggactct gggatcctgg aggcctgatg
     1261 ccagtgaata cagtaatgtt accaacgttt atcgcattac attgaaatcc cttgttcgac
     1321 gctatttcga gtttcatgat gaaatcacca acttggatgt catgattgcg gcaattgttg
     1381 atgaactggc acctgaactg attaaacgta atgctgttgg atacgaaagc gctttgcagt
     1441 tgttgatctc tgccggagac aatccctaac ggttaagaac cgaatcaggt tttgcggcgt
     1501 tgtgcggtgt cagccagttt cccctatctt ctggaaaaac gaaccattac cgacttaatc
     1561 ggggtgggga gcgtgttgca aatagtgcac tttacatcat cactatagga tgtttacaaa
     1621 ctgacgataa aacaaaggaa tatgtctcca ggcgagtagc cgaaggacat acaaaaatgg
     1681 aagcaatacg ctgtctgaag cgctatatct cacgcgaagt ttatacatta ctacgaaatc
     1741 aaaacaggca tgttaactgc atcccgataa cgtcttgact cttagaaggg cgtccaaggc
     1801 agtcattatt cgagcaggca gttccggcag ttattgtgtc gataccagat caggcagagt
     1861 atgagccggc gcggaaaatg ctgggataac agctcaatgg aacgcttctt caggagtctg
     1921 aagaacgaat ggatgccggt ggtgggttac gtaagcttca gcgaggcagc tcacgccata
     1981 acggacgata tcgttggata ttacagcgca ctaagaccgc acgaatataa cagtgggtta
     2041 tccccaaacg aatcggaaaa tcgatactgg aaaaactcta actcggtggc cagtttttgt
     2101 tgaccacttc agccatcgcc agacgctaac atgagttact cttgttctgg gctacgtcac
     2161 cttcagggaa ctggttatcc tcattcttta tttgtgacat tgtggttatt gtgcgacaaa
     2221 catggctatc ccgccccata tcccagataa aaccacctgc tcacgtgcga cagcgacaat
     2281 tgtaatattg acattctttc ctttggcctg aagttttcga gacctcttgc aaagcctgag
     2341 ttgagcatcc catgctctgt caatgacggg gcggggtaaa ttttcctgat tttttgtatt
     2401 gccggactga tgcgtgtcgg atgccggtaa ctccacgctg cctcaaccaa tagctttctg
     2461 gcatagctgt ttccggcttt ggtaatgctg ccctgatgtc tgcttccacc cgatgaatat
     2521 tctgatggcg tgataccaaa ccatgacatc agttgtcgtg gatgttcaaa acgggtcaga
     2581 tcacccagtt cagaaattaa tccaaccgca gtaataaact gaatccctcg catggcttgc
     2641 tgcgcctcta ctaccggata cagcctccat tcggtaacgg cctccttcgg gcggattcca
     2701 gccgttcaca ttgtgcctgc cggtcttcta ttgtacaacg gtgttcgtca aaagctaatt
     2761 gtcgccaggg actttcgaat gagtatttgc tgagccagtg tcggtgtgca gggccccaat
     2821 ctgctcgccc gacataatgg accccatgaa ccagaagaaa cgatttcaga cgttgccttg
     2881 catgccgtaa atcatcgcgg gcagatgccc atgcccgggc caaatcccgg aatgtctcat
     2941 cttcaacgcc ggatacgtag acagcagaaa gatctcctgc acgcagtgaa cgcacaagtc
     3001 tgatggcatc acggtgtcgg ttttaccccg ctccccctgt tttttcggga taagtgaggg
     3061 ggcgcagacc atgcagtcaa aaccggattt caccagccga cgataaagtc cataaccaca
     3121 gggtcccgct ttataaacga tactgacctg cgatgacttt gacctgatac gttgataaag
     3181 attatgaatg tcagtaggtg atgtgctaat tttacccatc agttcaacag gttctgaatt
     3241 gatagcatag gagacagtaa tcgattcttt gtgaacatct aattcgacgt acaacatgtt
     3301 attgttgttc atgctggcct ccgtggtgga aattccaggg gggtagcaac agctcaccca
     3361 tgcggctctg acaacgatgt taacccgcat taaatatccc acgttggaca gcatctgtct
     3421 cacggaaagt catactgcct tacgtgcata tttcacctta cgccttcact gtctgtcatc
     3481 ggaagtactg atttatattg gggctttgca cttactgtca ttcacgggaa aagatggtgg
     3541 tgaacagtac gatactgtct tggctgttgc cgagggaaga ttcaggagtg gtaagggggt
     3601 tagtggtgta agatgagtct ggagctctga ctggtcagtg acgcgaaact caatctgcca
     3661 gggtaaactc ccccttgctt ttgcgcagaa gcagtgggcc ttggttcgct tatgtcctgc
     3721 atctacaagc gaatgactga ccggagactc ccatatctgg tcagtcgccc atttttattt
     3781 tccaccacat cggacaagca taccgtaata aacagcacca gtctcctgtt cactcttgct
     3841 tgatttactg gaatgctgtt tatggagata tccggttgaa agattttacc tcttcgacag
     3901 gactataaat tatacagtaa gaagtccctc gtagaaaggg gctgattctc atcaagtcgg
     3961 acaggttcaa agtgttacct gaatgagcta aatgttattt accttccttt ctgttcattg
     4021 taaatttttc ttgctaacgc aaatcattat cattacgatt ttagaaaagg gaaatttatg
     4081 aagaacaaat atatcattgc tccgggcatt gccgtgatat gttctgcagt catatcatca
     4141 ggttatgcca gttctgataa aaaagaagat acgcttgttg ttactgcctc cggcttcact
     4201 cagcaactca gaaatgcacc ggccagtgtt tcagtcatta cttcagagca actgcaaaaa
     4261 aaaccggttt cagacctggt cgatgcggta aaagatgttg aagggattag tatcaccggt
     4321 gggaatgaaa aaccggatat cagtatacgt gggttaagtg gcgattatac attgattctg
     4381 gtcgatggac gacgtcagag tggtcgggaa tccagaccaa acggcagcgg cggttttgaa
     4441 gccggattta tccctcctgt ggaagcaatt gagcgtattg aagttatacg tggccctatg
     4501 tcttccctgt atggttccga tgctatcgga ggagttatta atatcataac caaacctgtt
     4561 aataaccaga catgggatgg cgtacttggg cttgggggaa ttattcagga acatgggaaa
     4621 tttggtaact caaccacgaa tgacttctac ctgtcaggcc cattggttaa ggataaactt
     4681 ggtcttcagt tatatggagg aatgaactat cgcaaggaag atagtatctc tcagggaaca
     4741 ccagcaaaag ataataagaa tataacggca acactccagt ttactccgac tgaaagccag
     4801 aagtttgttt ttgaatacgg aaaaaataac caggaacata cattaacacc tggtgattct
     4861 cttgatgcct ggactatgcg gggaaatctt aaacaaccaa acagtaaaag agaaacacat
     4921 aactcacgta gtcactgggt agcagcatgg aatgcccagg gcgaaatact gcatcctgaa
     4981 attgctgttt atcaggagaa agttattcgt gaggttaaat ctggtaaaaa agataaatat
     5041 aatcattggg atcttaatta cgagtcaaga aaaccggaaa taaccaacac gatcatagat
     5101 gcaaaagtga cggcatttct gccggaaaat gtgctgacca tcggaggtca atttcagcat
     5161 gcagaactcc gtgatgactc agccacaggt aaaaaaacga cagaaacaca gtctgtttca
     5221 gttaaacaga aagctgtttt tatagaaaat gaatatgctg caacggattc tctcgccctg
     5281 actggagggc tgcgcctcga taaccatgaa atctatggca gttactggaa tccaagattg
     5341 tatgctgttt ataacctgac cgataatctc actctcaaag gagggatcgc aaaagcattt
     5401 cgggcccctt cgattcgtga ggtcagtcct ggatttggaa cactgacgca gggtggtgcc
     5461 tctattatgt atggaaacag ggacctgaaa ccggagacca gtgtaaccga agagatcggt
     5521 attatttata ataatgacag tggtttttca gcaagcgcga ctctgtttaa tacagatttt
     5581 aaaaataagc tgaccagtta cgatataggc acaaaagatc cagtcactgg gctaaacact
     5641 tttatttatg ataatgtggg tgaggcaaat atcagaggtg tggagcttgc aactcagatt
     5701 cctgtgtatg ataaatggcg tgtatctgca aactatacat tcactgactc ccgtcgagaa
     5761 agcgatgacg aaagtctcaa tggcaagtcg ctgaaagggg aacctctgga aagaactccc
     5821 agacatgcag ccaatgcaaa actggaatgg gattacactc aggatattac attttattca
     5881 tctctgaatt atacaggaaa acaaatctgg gcagcacaaa gaaatggtgc caaggttccc
     5941 cgtgttcgta atggattcac ttctatggat attggtatga attaccagtt cctgccaggc
     6001 acgctgatta attttgccgt acttaacgtc acagacagaa agagcgaaga tatcgatacc
     6061 attgatggta actggcaggt cgatgaagga cgtcgttact gggctaatgt aagaatatcc
     6121 ttctgagaga atatagataa cctgaggccg tgcgcctcag gtaccagtat agtaatcagt
     6181 aacgtcagct tccaggttgt aatgctttta attttgataa aaataaactt aataaattac
     6241 atttgttaaa tattgataat tttaatttgg ttttattact ccagttatac tcacactcta
     6301 tctttacaca attcatcacc attacacctc catagcctgt tgatttaaaa gatttaattt
     6361 cctgtgaaac aatcaggcct gtatgaacct gattatacca cataatcttt taacttattt
     6421 aaatttaact ctcaggcttt tctgtcagta aaatcagaaa gaattgcagg atttcgtttt
     6481 tttgctgcga agtgaaatgt atctcatttt tactgccttc gccagtgcgt caggcaatgg
     6541 caggtagtgt acctgcattc tgctgtaacg cgtaatgtcg ggtattttac ttaatatgtt
     6601 ttatgtttgc attattataa a
//
LOCUS       DAZGXZ010000218         6609 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000218 DAZGXZ010000000
VERSION     DAZGXZ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.219"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..372
                     /gene="yjbE"
                     /locus_tag="ACHKJF_005008"
     CDS             130..372
                     /gene="yjbE"
                     /locus_tag="ACHKJF_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP0952221.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            486..1124
                     /locus_tag="ACHKJF_005009"
     CDS             486..1124
                     /locus_tag="ACHKJF_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP0952222.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1121..1858
                     /locus_tag="ACHKJF_005010"
     CDS             1121..1858
                     /locus_tag="ACHKJF_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0952223.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1858..3954
                     /locus_tag="ACHKJF_005011"
     CDS             1858..3954
                     /locus_tag="ACHKJF_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0952224.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4001..4279)
                     /locus_tag="ACHKJF_005012"
     CDS             complement(4001..4279)
                     /locus_tag="ACHKJF_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952225.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4295..4423
                     /locus_tag="ACHKJF_005013"
     CDS             4295..4423
                     /locus_tag="ACHKJF_005013"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952226.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            4549..4959
                     /gene="psiE"
                     /locus_tag="ACHKJF_005014"
     CDS             4549..4959
                     /gene="psiE"
                     /locus_tag="ACHKJF_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP0952227.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5003..6478)
                     /gene="xylE"
                     /locus_tag="ACHKJF_005015"
     CDS             complement(5003..6478)
                     /gene="xylE"
                     /locus_tag="ACHKJF_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP0952228.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
BASE COUNT         1634 a         1681 c         1774 g         1520 t
ORIGIN      
        1 attccaatta tcctaaaacc ccatctctaa ttccccgcgc cgtaattcgc atgctttagt
       61 tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat tcaatgaagg
      121 gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct tgcggcgact
      181 tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc aacgtcggtt
      241 tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt gggtgtcgcg
      301 ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac cacgaccacc
      361 agtacccagt aataaagtat gtatccccaa aataatttga gtcattgcat ctgtggcttg
      421 aagtatgaag ggatttacca taaccacact ccggtgtggt tattcagccc ctctggagaa
      481 gagtcgtgaa gcgacctgca ctcattctta tctgcctgct attacaggcc tgttcagcca
      541 cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca ggcgtacagc
      601 tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag cttaatggcg
      661 ggccgcaatt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa tgggtcactc
      721 aggatcaagc cactcttgtc acacaacatg gtcgtctggt gaagactttg cttggcggcg
      781 acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc gcacaaattg
      841 ttgatggtgc aacatggacg cgcacgatgg gctggaccga gtaccagcag gtacgctacg
      901 ccaccgcacg ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc ggcagcgatg
      961 aaaccccggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc tggcataacc
     1021 gctactggat cgacagcgaa gggcaaattc gccagtcgga acagtatctc ggcgcggatt
     1081 atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac aaactattgt
     1141 cgcattgatt ttgagtgtgg gggcgtcatc ggtcttcgcg gcgggaaccg tcaaggtatt
     1201 cagcaatggc agcagtgagg ccaaaacgct gacgggcgca gagtatttaa tcgatctggt
     1261 aggccagccg cggctggcaa acagttggtg gcctggtgcg gtgattagcg aagagctggc
     1321 aacggcggca gcattgcggc agcagcaggc gttgctgaca cggctggcag aactggctgc
     1381 agattccagc gccgacgatg ccgccgcgat taacgcctta cgccagcaaa ttcaggcgtt
     1441 gaaagtgacg ggcaggcaaa aaatcaatct tgatcctgat atcgtccgcg ttgccgaacg
     1501 cggtaaccca ccgttgcagg gcaactacac gctgtgggtc gggccaccac cgtccacggt
     1561 cacgctgttc gggcttatca gccgtcctgg caagcagcca ttcactccag gtcgcgacgt
     1621 ggcgagctat ctctctgacc agagcctgct cagcggtgcg gatcgcagct acgcgtgggt
     1681 cgtttacccg gacggacgca cacaaaaagc gccggtggct tactggaaca agcgtcacgt
     1741 agagccgatg cccggcagca ttatttatgt tggcctcgcg gactccgtct ggagtgagac
     1801 gcctgatgcc cttaacgccg acattcttca gactctgacg cagcggatac cccaataatg
     1861 aaaaaaagac atctgcttag cttactggcg ctgggcatta gcacagcttg ctacggcgaa
     1921 acatatcctg cgcccattgg tccgtcgcag tcggatttcg gtggcgtagg attattacaa
     1981 acgcccaccg cgcgcatggc gcgggaaggg gagttgagcc tgaactatcg agataacgat
     2041 cagtaccgtt attactcagc ttcagtgcaa ctcttcccgt ggctggaaac aacgctgcgc
     2101 tacaccgacg tgcgcacccg gcagtacagc agcgtcgaag cgttctctgg cgatcaaacg
     2161 tataaagata aagccttcga tctcaaactg cgtttgtggg aagagagtta ctggctgccg
     2221 caagtggcgg ttggtgcgcg ggatatcggc ggtacggggc tgtttgatgc ggaatatctt
     2281 gttgccagca aagcctgggg gccgttcgat tttacgctcg gcctgggctg gggatatctg
     2341 ggcaccagcg gtaatgtgaa aaatccgctc tgttcagcca gtgataaata ttgctatcgc
     2401 gataacagct acaaacaggc gggatctatc gacggtagcc agatgttcca cggtcctgcc
     2461 tcactgtttg gcggcgtgga ataccagacg ccctggcaac cgctgcgcct gaaactggag
     2521 tatgaaggca ataattatca gcaggatttt gccgggaagc tggagcaaaa aagtaagttt
     2581 aacgtcggtg cgatttatcg cgttaccgat tgggccgacg ttaaccttag ctatgaacgt
     2641 ggcaacacct ttatgtttgg cgtcacgctg cgcaccaact ttaacgatct gcgcccgtcc
     2701 tacatcgata acgcccgccc gcaatatcaa ccgcagccgc aggatgccat tttgcagcat
     2761 tcggtggtgg cgaatcagtt aacgctgttg aaatacaatg ccggacttgc cgatccgcag
     2821 atccaggcga aaggcgatac gctgtatgtt accggcgagc aggtgaaata tcgtgattcg
     2881 cgcgaaggga tcatccgtgc taatcggatc gtgatgaatg atctgccgga tgggatcaaa
     2941 acgatccgcg ttacggaaaa tcgccttaac atgccgcagg tgacgacgga aaccgatgtc
     3001 gccagcctga aaaatcatct cgccggagag ccgttgggcc acgaaacgac gctggcgcaa
     3061 aaacgcgtcg agccagtggt tccgcagtcc accgagcagg gctggtatat cgacaaatca
     3121 cgctttgatt tccatatcga tccggtgctg aaccagtcgg tcggtggccc ggaaaacttt
     3181 tacatgtatc agctgggcgt gatgggaacg gcagatttgt ggctgacgga ccatctactg
     3241 accaccggca gcctgtttgc aaatcttgcc aacaactacg acaagtttaa ctacaccaat
     3301 ccaccgcagg actcgcactt accgcgcgtt cgtacccatg tgcgcgagta tgtgcagaac
     3361 gatgtctatg tgaataacct gcaagccaac tacttccagc atctgggcaa cggcttctac
     3421 ggtcaggtct acggtggtta tctcgaaacc atgtttggcg gtgcgggggc agaagtgttg
     3481 tatcgcccgc tggacagcaa ctgggcgttt ggtctggatg ccaactacgt taaacagcgc
     3541 gactggcgta gtgcaaaaga tatgatgaaa ttcaccgact acagcgtgaa aaccggacat
     3601 ctgaccgcct actggacgcc atctttcgct caggatgtgt tagttaaagc cagcgtcggg
     3661 cagtatctgg caggggataa aggcggcacg ctggagatcg ccaaacgctt tgatagcggc
     3721 gtggtggtgg gtggctatgc cacgatcact aatgtttcaa aagaggagta cggcgaaggg
     3781 gacttcacca aaggcgtgta tgtctcggta ccgttggatc tcttctcgtc tggcccgaca
     3841 cgcagccgtg cggcgattgg ctggacgccg ctgacgcgtg acggtggtca gcaacttggg
     3901 cgtaagttcc agttgtatga catgaccagc gaccgtagcg tcaatttccg ctaagtcatg
     3961 ggaaaggtgc cagttttcgc aatcactggc accctcattc ttaatggcct gttgagccat
     4021 caggaacgca gttcaccccc ggtttgcgat ctggtgtgta aaggcgaaaa cattctgtct
     4081 tcctgtccgt cgatatcggt tgcagatctg gttgcctgag cgactgggcc atcattgggc
     4141 tggagacatt gctttgcggt aggtcggctt tatcagcagt accgagcgga ccagcataag
     4201 caggaagaac agagactgat aacatcaaag cagcaaaata aggcttcatt tttaccacct
     4261 ttatcaggtt acgtttcatt tgttccagag gaacatggtc gatttttcgc gcattgctgg
     4321 tggctgggaa tcacctgaat gggtgatttt tgaattaccg gctttggtgc ggtttgtctt
     4381 gccggatgcg ccgccaggcg cggcttatcc ggcctacggg taggtatatc cggctttggt
     4441 ggaggcgcgc tccaaatcca ggttgaacaa aacatacaca aaaaatatag atctccgtca
     4501 catttttgcg ttatacagga agctcgccac tgtgaaggag gtactgctat gacgtcactc
     4561 tctcgtccgc gcgtggagtt tatctccacc attttgcaga ccgtactcaa tcttggcctg
     4621 ttgtgcctcg gcctgatttt ggttgtcttc ctcggcaaag aaacggtgca tctggcagat
     4681 gtgctgttcg cgccagaaca aaccagcaaa tatgagctgg tagaaggact ggtggtttac
     4741 tttctctatt tcgaatttat cgcgctgatt gtgaaatact ttcagtccgg ttttcacttc
     4801 ccgctacgtt actttgtcta tattgggatc accgccattg tgcgcttgat catcgtcgat
     4861 cataaatcgc cactggatgt gttgatctac tcggctgcga tcctgttact ggtgatcact
     4921 ctgtggttgt gtaattcgaa acggctgaag cgggagtaaa aagacagcac gccgaactgg
     4981 cgcggcgtgc tggacaggaa gattacagcg tagcagtttg ttgtgttttc ttcgtttccg
     5041 gttcccagag ggcttccagc tcctcaaggg ttttaccttt ggtttccggg acaaatttcc
     5101 acataaatag tgctgccaga acgcccatac acccgtaaat ccagtaggag aaaccgttgt
     5161 ggaaatgggc caccagccag gagtttttgt ccatcatcgg gaaggtccag gagacgaagt
     5221 agttcgccag ccactgggcc gccaccgcga ttgccagcgc tttaccacga atggcattcg
     5281 ggaagatttc cgacagcaga acccagcata ccggacccca ggacatggca aaggcggcaa
     5341 catagaacag catcgacagt agcgccacaa tacccggtgc ctgagtgtaa aacgcggtac
     5401 cgaggctaaa cataccgatt gccattccga gcgcgccgat aatttgcagt ggcttacgac
     5461 caaatttatc caccgtcata attgccagaa cggtgaaggt gaggttgata actccgacaa
     5521 taatggtctg caacagcgcg atatccgtgc tggcccccag cgttttgaac acttccggcg
     5581 cgtagtacaa caccacattg atgccgacaa attgctggaa gatggagagc ataacgccga
     5641 tgacaatcac gcccacgcca aacatcagca gacgaccacc ggttttgcgg ccatgatcca
     5701 gggagtgttt aatttcctgt actgcctgag ttgcaagcgt gttgcccata attttgcgca
     5761 ggatactttc cgcctgttct tgcttgccgc gtgacatcag ccagcgagga ctttctggca
     5821 cggtatacag cagcattaag aacagcagtg cagggataca ttccgaggca aacatataac
     5881 gccagccgtc agtattcagc cagctggcat caccggaacg ggcaataaaa tagtttacgc
     5941 agtaaactaa aagttgcccg aaaataatcg caaactggtt aaatgaaacc agttttccgc
     6001 gaatatgagc tggagccagt tccgcaatat acattggtga aagcattgag gctaaaccaa
     6061 cgccaatacc gccaataata cgataaataa caaattccgg gacataacct gccagataaa
     6121 taggtactgt gttgtccggg tttatagagg taaaaccaag ttctggccag gcagaaccta
     6181 caccagaaat aaaaaacagg acagcagcaa tcttaagtga atcacgacgg ccgaagcggt
     6241 tactgcaata accaccgagg gcaccgccga tgatgcaacc aatcagagcg ctggctacgc
     6301 aaaatcctaa cagggagttg gcagcggatt cacttaagtt ttgtggagca acaaagacgg
     6361 tattgagtga ctcaacagta ccggaaataa cggcggtgtc gtagccaaat aataaaccac
     6421 ctaatgtagc gactaaggta atcgaaaata tataactgga attatactgg gtattcattc
     6481 agacctgcct tagaccattc tgatctttta tcagtgcatt gatgttggaa ttgtcaggca
     6541 gttaaggcaa ttatcatttt ttacaatgcg tttacgtaat gtcttttctg tgatcttaat
     6601 tgtgataat
//
LOCUS       DAZGXZ010000219         6606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000219 DAZGXZ010000000
VERSION     DAZGXZ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.220"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..224
                     /locus_tag="ACHKJF_005016"
     CDS             <1..224
                     /locus_tag="ACHKJF_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952229.1"
                     /translation="IKNRKSTKESKIYAACGLYYLNVENIESLFNENDKQEYVSVLRV
                     DILTKIKLNDILNSVIINGCNTKLISEHK"
     gene            763..2037
                     /locus_tag="ACHKJF_005017"
     CDS             763..2037
                     /locus_tag="ACHKJF_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP0952230.1"
                     /translation="MIPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESAT
                     LPVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLT
                     LVLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVL
                     SLDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAE
                     TWFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQ
                     LYRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSP
                     ILFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTV
                     YTGGHDYAWWRGAIIDELANYNCRMVSNNNSV"
     gene            complement(2185..3303)
                     /locus_tag="ACHKJF_005018"
     CDS             complement(2185..3303)
                     /locus_tag="ACHKJF_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP0952231.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAEEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSRIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGK"
     gene            complement(3978..4214)
                     /locus_tag="ACHKJF_005019"
     CDS             complement(3978..4214)
                     /locus_tag="ACHKJF_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0952232.1"
                     /translation="MLTSITGHDSVLLRVDDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            4315..4901
                     /locus_tag="ACHKJF_005020"
                     /pseudo
     CDS             4315..4901
                     /locus_tag="ACHKJF_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4952..5131
                     /locus_tag="ACHKJF_005021"
                     /pseudo
     CDS             4952..5131
                     /locus_tag="ACHKJF_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515829.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5287..5464)
                     /locus_tag="ACHKJF_005022"
                     /pseudo
     CDS             complement(5287..5464)
                     /locus_tag="ACHKJF_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(5575..6141)
                     /locus_tag="ACHKJF_005023"
     CDS             complement(5575..6141)
                     /locus_tag="ACHKJF_005023"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0952233.1"
                     /translation="MYTESNVKLSSNALWQKCVLATLYRAAATHPEIKLLQFKLIPCP
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPTPLYYLWSREAVANDTTPYLM
                     VLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDNSITHLVVYPEVLSLVFDAANTYNA
                     EPGVLEQTARAYIESHGAIERHLGCSDF"
     gene            complement(6169..6366)
                     /locus_tag="ACHKJF_005024"
     CDS             complement(6169..6366)
                     /locus_tag="ACHKJF_005024"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0952234.1"
                     /translation="MPQDDFCIVNPGGCVIHFPANGRDIQDIHDPDFPEQYQRLLERL
                     DYLTKPGTKASGQRNSGYSGF"
BASE COUNT         1984 a         1445 c         1398 g         1779 t
ORIGIN      
        1 taataaaaaa tagaaagtca actaaggagt ctaaaattta tgctgcttgt gggctatatt
       61 atttaaacgt agaaaatata gagtcattgt ttaatgaaaa tgataaacaa gaatatgtgt
      121 ctgtcttaag agtggatatt ttaacaaaaa taaaactgaa tgatattctg aattctgtga
      181 taataaatgg ttgcaacacc aaattaatat ctgaacataa atgagcagga ggtctttaaa
      241 atgagagaaa tatcagatca catgcttgat ttcgtgaaag gagggatgaa tcttaatgga
      301 ttacctagtt ctactaatgt aatagatcta cgaggaaaag atatgggaac atatattgat
      361 gctaatggag catgctgggc tccgggtact ccatccatca tcatgtatcc ggggggaagt
      421 ggaccttctt atagtatgag tagttccaca tccagtgcaa acagcggcag ttaaataaat
      481 accctccggc atggccggag ggtatttatg atgtgcctgt aagactctac ttctgtcgtc
      541 tctttacaaa ggcatatgat ctgtcatttt gtaccagaca tcttaacgag agccttttca
      601 tatattactt acatacgtga aatctacagt ataaagcaaa aattttatta aacctgtttt
      661 atatttatca tcaatacaac agatttaatt ccggaaagtc actcatcatc ccttgaatga
      721 acgtcatttg atgtattatt cttacacttg gattaattta agatgatacc tatgaaaaac
      781 tatcttttcc agacacccga agatatttgt gtacagttaa aaaaaatgac acatcctgtc
      841 acaataagaa caacagatat tgctaatttc tggcactatc ttgagtcagc aacacttcca
      901 gtgatcacaa aaagcaccac tacagaaaat cgggaggtta catttctgtg gcgctcagag
      961 aaagcactgc aaggcgtata tcttcgcctg aatcgtgtaa cagataaaaa agacgtcaaa
     1021 aaaggactaa tgactcatat cccttcgaca gatatctgga tgctgacact ggtgttacca
     1081 gcttcatatc ggggctcata ctcatttata gaaattccca cagatatgac acaaaaagac
     1141 atatttcaac taggaagtcg cttctctcca ttacccggta aatctgatcc atttaacaaa
     1201 acagcagaaa taaatatacg aggattcgga gaatcagtcc tttctcttga tatggctcct
     1261 gaacaaaagg aatgggatga tacttcccat aaatgtacag gtattctttc aacattacat
     1321 tcctttgttg caggatatca acgccggatt cgtttatatt ttcccctgaa tccaacatca
     1381 gtacctcttg gattacttgt gttacctgat gctgaaacat ggtttgaccg gatagatatt
     1441 acccgggcat tagatatggc catcaccact ggtcgtatca cgccaatggc aattatgggg
     1501 atagacaata ttaatgaatc tgatcgtatg aatatactgg gaggcaataa agaactcatc
     1561 tttgatatag cggaaaatct gataccccag ttatacagag actatccgga tatcgtatgg
     1621 gctggtcgct ctaatactgt actggccggg cagagcctcg gtggagtgac agcactgatg
     1681 gcagctatat atgcgtcgac aacatttggt acaatcatta gtcactcacc ttcaatgtgg
     1741 tggaaccctg accagggcag cccgatcttg tttactgaga ataatacctc ctgggtaagt
     1801 gagcagatac tttcagcgcc tccgaaagat gtaaatatcc gacttggagt cggttcttta
     1861 gaaggtacaa ccgtctcaca tgttcagcga ttgcatcatt cgttaatcgc agctggtttg
     1921 gaaagtaacc tcactgtcta taccggtggt cacgattatg cttggtggcg cggagcaatt
     1981 attgatgaat tagcaaatta taattgcagg atggtatcaa ataataactc tgtgtaaaat
     2041 atcgttttat cgtccgataa ctctttctgc aatattatta tgggagactg tctcatttat
     2101 ttagtaatgt ccttcattta tgtgatgaag gacattatat tactcataac atttctcgtt
     2161 gaaaatttca gcaatacgct acaattattt cccgttgttt tttaattttc tcatcaaaac
     2221 ctctgcgatc tctgcaggac tgggttgttc agccatttca gcggctacct gatctgaaca
     2281 gaagcgtagt gaatcatcat aaagcaggcg atttaccaaa tcactggtca gtccatgctt
     2341 gtccggaata atcccacacc cacgcttcgc aacaatttct gcattaacag agcgatctgc
     2401 accttcgcca aacacaatct gtggtattct actatacagc gctgtcaggg tattacctgc
     2461 gccaccgtga tgaataaatc catcagctcc attaaggaat acacccatag gtatccagtc
     2521 aaccagacgt acatttgatg gtagttttcg taaaccagta cgagcattta ttgcaagttg
     2581 caaaatgata tcagcatcaa cttcatttgc agaatccata acccatgaaa tcagctccag
     2641 accatcaacc attggtttta cagtgccaag gctgataagt aaacgtttcc gatcagaatc
     2701 cctgtcccac catgtttcct ttacagcacc tccgttataa ggaatatatc tcattgagat
     2761 aaccggttct tcagcatttt taagaatgct catacttggg ggagcaacat caatccatgc
     2821 taaatcacat agtggaccgc tgactccatg ccgcctgtaa gcattgctta aagaacggtt
     2881 taacatctga atatgggcag atgtatgcgc gaatccaaca gccagcatca ctgaaggaat
     2941 tctatattta gcagcaacca atgggcctgc cggaccaagc gggggataga ctattaaatc
     3001 tggcctccat tttcctgcaa aatcgatgag gttatctgcc atttcatcgc taaaaaaaga
     3061 gaaatgacca ataatattac tttttttcct caactcttcc tgatggcgat atccagcctc
     3121 tgaatctaaa cctggaactg catcaaaaac aaccagtcca gcttctgctg ctttattcgc
     3181 gaatttgcca gcgctactaa ttactacatc atgtccgatt acacgaaagg cctgagccag
     3241 agaaatcaat gggtataaca aaccgtacag cggagggcca ataaaaagaa tacgttttcg
     3301 cattaaaaaa gtccgtcttc tgcttacctg tttatttttc tatcgctaag aaaacataat
     3361 taatattatt tttatgtgag cttatgcaca aaaataacaa agtatcttat cttctggtag
     3421 agatacagga tacagtttgt aggagctttc ttttttaaac aatgatttgc aggacagtta
     3481 tgccaaccta gcagaatcag tatcacaaca ataaatgaca gatcatacct gaaatttgaa
     3541 ttaacaggca tcatgaatgt caatagtcta agcggttact acttacatcg cttgcataaa
     3601 tactaaacac tgactagaaa agtattggat aaccccgtca ctaaaagtaa cacttcggtt
     3661 atttgctcgg aatgtatcaa cgccaagatt gttagccgtt acaccttata tcactggcgc
     3721 tgacaaatgt ttcttttcta atttactgaa ccggcgcaga cttacagaaa aacactccgg
     3781 caccagtgta atgccagtta taatcaataa gaataaaatg acgaatcagt caggaatagc
     3841 tataacctgc aggctgactg gagaatgtct gcatgaagat gaacgtaaat gtcgccagca
     3901 ccaggggcag ggccgggttc cacaatacca gcgctggcat tttgcacatt gccagcgtac
     3961 cggcattaca acacgtttca tgctgctgct cctcgtgact cctcgattcg ctgttgcatc
     4021 cactgctcca cttcgctttt gtaccagcgg ctgctgcggc ccagcttgat gggtttagga
     4081 aaatgtcctt cactgatcag cttgtaaaac catttgtcag tcatacctgt aaagctggtg
     4141 atgtagttca tgtcgatcag gggatcgtcg acacgcagca atacgctgtc gtggcctgtt
     4201 attgaggtca acatggtcag aatatcctca gtgagattta ttaagaatgg ggtaatacgg
     4261 ggaatgggat ggcgtgctgc tttcagcact gccaaccggg attgccggca aggcaagggg
     4321 cttctggacc catggaggga gtggttaaaa gagcaacgag aaagtggtaa ttataacgcc
     4381 agccggatat ggcgggaaat ggtggcccgg gggttacagg cagtgaaacc atcgtcaggg
     4441 atgctgttgc caaatggcgt aaaggctgga acccaccagt tactactgcc gccagacttc
     4501 cttcagtgtc ccgggtaagc cgctggttga tgccctggat aataatcagg ggtgaagaaa
     4561 attatgcttc ccgatttatt agtctgatgt gtgaaaaata accggagctg aaaatagcgc
     4621 agcaactggt actcgagttc taccgtattc tgaaaaccca aaatagatca cagctaagca
     4681 gctggttcac tcgagtccac gaaagcggtt cagcagaact tcggcgcgtg gcggcgggga
     4741 tggaagctga tgctgcggct atatgtgagg caatcagcag tagctggagt aatggtgttg
     4801 tcgaaggtca tgtaaatcgc ctgaagatgt tgaaacgcca gatgtatggt cgagccggat
     4861 ttgaactact caggcagagg gtcatgagtc cactggcatg aaaatctcca cccgggttgc
     4921 ggaagagcca ctattgcacc gttcgtgaca cattgggcaa actaaagatg tgtatgtcta
     4981 ttacccaact gtgagagata ctgaaatcac aacatttgaa gaaactctgg ataacctcct
     5041 acaacgccga cgagcattgg ccagagatat gctctgcgca actcccgatt tgaactgtgc
     5101 ggattttgag acccttttga agggagccta accgatatga gcggcaatac tgccagttgc
     5161 caaacctgaa ataacactta gctgtgatga ttcgctatta cctaccgata gcttcggaaa
     5221 acataaatta aaaatgccca gaggagtact attatcgtta gcattacccg aacgccgtat
     5281 taacggttat ttattggtaa gtgctacgtt ccatggcaag agttcatcaa cacggttagc
     5341 cgacagtata ttgagtatag ggcgcagata tgcttgcgga ttgataccat tcagacagca
     5401 ggtcccgctc agcccgtaca gtagagcgcc gttcactacc gtaatcactg ccgaaaaaca
     5461 cattgattgc ccctcaataa tcctgccgtc aacgtcaaaa ttgctggtcg ggctcgactt
     5521 atttaattgt gtaaacaggg cctctcagcg aaggccctta tgcatcacct gacgttaaaa
     5581 gtcactacac ccaagatgac gttcaatggc accatgtgat tcaatgtaag ctcgggccgt
     5641 ctgttccagt acaccaggct cagcgttgta tgtgttagct gcatcaaata ccaacgacag
     5701 cacttcagga tacacaacca gatgtgtaat ggagttatct tcacccaata cttttcccca
     5761 cgcctgctca atcagatttc tgagaaccac cacctcacga ctcttacacc agacatcgtt
     5821 attaagtagc agcaccataa gataaggagt ggtatcgtta gccacagcct ccctactcca
     5881 gagataatat aaaggggtgg gctcaacaga tttatcttta cgtcgcttac actgcaaata
     5941 ttcagaaatg agtctatgca gttcaccagt aaaatccgcc atcagagagg gaatggcctt
     6001 attaatacca gggcaaggta ttaatttaaa ttgtaataat ttaatttcag gatgtgtggc
     6061 tgcagcccga tacagagttg caaggacaca cttttgccag agggcgttac tggaaagctt
     6121 aacgtttgat tctgtataca taataaatca ccttacagtt acaacaggtc aaaaaccgct
     6181 gtagccagag ttacgctggc ctgatgcttt agtaccgggc ttcgtcagat aatccagacg
     6241 ctccaataag cgctgatact gctcagggaa atcaggatca tgaatatcct ggatgtcacg
     6301 tccattagca gggaaatgaa taacgcagcc ccctggatta acaatgcaga aatcgtcctg
     6361 aggcactgat caatacggag aggactctcg cgtatggttt attgacacca cagtgcaaat
     6421 tcggcgaatc cgcgatcacg gtgcgatttc gttccacagc acacaatcat gaccccgggt
     6481 tttattcagg taagcaggat tgcggatatc cggtgtcgcg cctttgtgtc acgaacgggg
     6541 taggtgcgaa acaccggata aaatgcaggc aggcaatacc tctgaacgcc ctgcgcagag
     6601 cggata
//
LOCUS       DAZGXZ010000220         6566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000220 DAZGXZ010000000
VERSION     DAZGXZ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.221"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..165)
                     /locus_tag="ACHKJF_005025"
     CDS             complement(<1..165)
                     /locus_tag="ACHKJF_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952235.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRAD
                     SRFELIQKNVG"
     gene            complement(343..1254)
                     /gene="gsiD"
                     /locus_tag="ACHKJF_005026"
     CDS             complement(343..1254)
                     /gene="gsiD"
                     /locus_tag="ACHKJF_005026"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP0952236.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1257..2177)
                     /gene="gsiC"
                     /locus_tag="ACHKJF_005027"
     CDS             complement(1257..2177)
                     /gene="gsiC"
                     /locus_tag="ACHKJF_005027"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP0952237.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2195..3733)
                     /gene="gsiB"
                     /locus_tag="ACHKJF_005028"
     CDS             complement(2195..3733)
                     /gene="gsiB"
                     /locus_tag="ACHKJF_005028"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP0952238.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3753..5624)
                     /gene="gsiA"
                     /locus_tag="ACHKJF_005029"
     CDS             complement(3753..5624)
                     /gene="gsiA"
                     /locus_tag="ACHKJF_005029"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP0952239.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5611..>6566)
                     /gene="iaaA"
                     /locus_tag="ACHKJF_005030"
     CDS             complement(5611..>6566)
                     /gene="iaaA"
                     /locus_tag="ACHKJF_005030"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0952240.1"
                     /translation="VIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLEAGES
                     ALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSHLRNP
                     VLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEGATVL
                     DHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGCYANN
                     ASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGGLIAI
                     DHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
BASE COUNT         1631 a         1913 c         1567 g         1455 t
ORIGIN      
        1 gccaacgttt ttctgaataa gttcaaagcg gctatcggca cgttttaccg cgcgttccga
       61 taccagttga tagttaatga tgaaaaaaag accaataaat gacagtgctg ccaataaaaa
      121 taaaattatc agccttatct ttatcttttc gtataaactc agcatgttaa tacatttaac
      181 aaaataacta tctgaacgct gtgccgctgc aaaaatagtc ccttgctgcg gctttttaac
      241 caaattaccc gattctacaa aatcagcaga aagttacaac acttgcttag ggcaataatt
      301 gcctgtgagg tgattaccct ttcaagcaat attcaaacgt aactatcctt taattttcgg
      361 atccagcgca tcgcgtaaac catcgcccaa caaattgaac gccagtacgg tcagaaaaat
      421 agccagggcc ggaaaaacag cgacatgcgg cgcgataacc atatccgctc gtgcctcatt
      481 gagcattgct ccccactctg gtgtcggcgg ctgcgcaccg aggccgagaa atgacaggct
      541 ggcggcagag ataatcgagg tgccaatgcg catggtgaaa aacaccacga tagacgagac
      601 ggtcccaggc aagatatgac gcaataaaat ggtcatatcg ctggcaccaa tactgcgtgc
      661 tgactcaata aaggtttgct gtttcaacac cagcgtgttg ccgcgcacca ggcgggcaaa
      721 cgcggggatg gaaaaaatgg cgactgcgat aatcacgtta gcaatgccgc ttcccaacac
      781 cgcaacaaca gcgatcgcca gtaaaatacc cgggaaggca aacagcacat cgcaaatgcg
      841 catgatcagc cgatcccacc agccttcata atatccagcc agcaagccca gcaacgtccc
      901 tatcgccgca ccgataaaca cggcaaacac gcccgccgcc agcgagattt gcgcaccaac
      961 caggacacgg ctgaaaatgt cgcgccccag tgaatcgacg ccaaaccagt gctgcaaaga
     1021 aggtccgtta ttcagattgt cataatcaaa ataattttcg gcgtcatagg gagcgatcca
     1081 gcgtgcaaaa atggccacca caatcaataa aataacgaat aatgcggcgg tcatcgccat
     1141 atgctgacgg cgaaatcgtc gccagaattc atgccacggt gtacgcacct ggtcaggttt
     1201 gaccagtggc atggcgttta acaccgcctg acgtcgccag ttaaatagtc gcatccttac
     1261 ttgtacctga tagccgggtt aatggcggcg taaagcacat ccaccactaa gttgataaga
     1321 ataaattcca gcgagaaaag cagaatttcc gcctgaatca ccgggtaatc gcgcatttct
     1381 acggagtcaa cgagtaagcg tccaagtccg ggccagttga aaactttctc caccacgata
     1441 gaaccaccga gcaaaaagcc aaactgtaag cccatcatgg tcactaccgg gatcatcgcg
     1501 ttacgtagcc cgtgtttgag gacaacccag gtttcgctca cccctttcgc cctcgcggta
     1561 cgcatataat cttcgcttaa aacatcgaca aacgacgcgc gggtaaagcg cgccatcacg
     1621 gctgccactg ccgcgccgag cgtcagggag ggtaaaatgt agtgctgcca actgtctgct
     1681 cccacggtag gcagccagcc cagttcaacg gagaatacct gaattaaaag catccccagc
     1741 gcaaatgccg gaaacgagat ccccgacacc gcaatggtca tactcaatcg atccggccaa
     1801 cgattacgcc agacggcggc gataattccc gccgccatac caaatatcac cgcccagacc
     1861 atactggtta tggtcagcca cagcgttggc ataaagcggc tggcaatctc atcggcaacc
     1921 ggacgacgcg acaccatcga caggccaaaa tccccctgca cagcattgct gatatagtgc
     1981 cagaactggt gatacagcgg ctgatccaac cccagctgct gacgcaccag ttctataacc
     2041 tgcgcatcag cttcgggccc ggcaatcaat cgcgccggat cgccgggcag catatgcaca
     2101 aataaaaaca ccagcaccga gacgataaac agcgtcggaa tcaaccccag taagcgtttg
     2161 ataacgtaat taagcattcc actccctgcg ttgcttattg caaatccgcg tcttcaaagc
     2221 tgaagccggt gtctggcatg atccaaaaac cggtcaggtt tttactgtgt gccgacacca
     2281 gtttttctac caccagcggg atccacggcg actcttgcca gatgatatcc tgcgccgcct
     2341 tatacaagcg ggtcttttcc gccggatcat tagttttcag tgcctgagcc aggaagtcgt
     2401 ccacctgttt attgctgtaa aatgcggtat taaacagcgt cggtggccag ttttgcgagg
     2461 cgaacagtgg cgatagcgcc cagtccgctt cgccggttga agccgaccag ccagtgtaga
     2521 acatccgcac gccgctctct ttttgccctt taccttcaac ttctgccgcc cgctgtccgg
     2581 catccatcgc agtcacctgg actttaatcc cgacctgcgc taactgctgc tgggtaaatt
     2641 gcagcacttt ctgcgcggtg ctgtggttat gtgacgacca cagcgtggta ctgaaaccgt
     2701 tgggatatcc cgcctctttc agtaattcgc gcgctttcac tggatcgtaa ggccacggtt
     2761 tataactttg cgcgtaggcg atacttggcg gtaccacacc agtagctggc gttgcatagc
     2821 ccgcaaaggc aactttcacc agcgccggac ggttaatggc gtaattcagc gcctcacgga
     2881 ccttcgggtt atcgaacggc ttttgcgtca cgttcatact gatataacgc tgcataattg
     2941 acggactggc catcaactcg atatttttgt ttttctccag cagtgtggcc tgctcgtagg
     3001 gaatggggaa agcaaactgc gcttcaccgg tttgcagcat tgccgcgcgg gtgttgttat
     3061 ccgccaccgg acgccaggtt atgctgtcca gtttgggcaa tcctggctgc cagtaacccg
     3121 cgaatttttt caccttcaca aaatcggtct gattccaggt atccagttca tacggtccgg
     3181 ttcccaccgg atgaaaacca atctccttgc catatttttc cagcgctgcc ggtgaaatca
     3241 tcgcggtcgc cggatgggca agaatattaa taaacgctga gaacggctgt ttgagggtaa
     3301 tctttactgt tgttggatcg atcgcctccg ttttggcaat attcttatac aggttatagc
     3361 gtttaagatg attcgccgga tcgctggccc ggtccagatt cgctttcacc gccgcggcgt
     3421 tgaaatcggt gccatcctgg aatttaatgc cttcccgcaa tttcacggtg taagtaatgc
     3481 cgtcatcgga aacggtataa ctctccgcca gcacgttttt cagtttcatc tctttatcca
     3541 gaccgaacag cccctggtaa aacgatttcg ctacggcctg agataacgtg tcattcgcgt
     3601 catacggatc gagcgtggtg aaattcgatc ctaccgccac caccacatct ttggcagcga
     3661 atgcacaaga tgccatcaac gctgtcgcaa tgcccagcgc cactaaccca ctacggtgta
     3721 cagctcttgc cattttattc tccgcctgaa tgttatctac gcatgaatgc gtattctgat
     3781 tgtggttgtg cgacgtaatg ccccggcccg acgcattgca acgagacggc tgccacctct
     3841 tcgccacgca gatgaatatt gctgggaaga tcgtccgaca gcagtacacg ctgcggtcgt
     3901 tgtcgggacg gttcagcgac cggaactgcc gccagtaatt tacgcgtata aggatgctgc
     3961 gggttttcga agaccgcgcg ccgtggacca atttcaacaa tttgcccgag atacatcacc
     4021 gccacacgat gactaatccg ctctaccaca gccatatcgt gggagataaa cagatacgca
     4081 atgccgaaat cacgctggag atcgagcaac aagttgataa tctgcccgcg aatagaaaca
     4141 tccagcgccg aaacggcttc gtcggcaatg atcacttttg gattcaatgc caacgcgcga
     4201 gcaatgcaga tgcgctggcg ctgaccgccg gaaaactcat gcgggtaacg ccaggcatgt
     4261 tcaggtaaca ggcccacgcg ctccagcaac cacgcaacgc gtgcagccgc ttctttacct
     4321 ggcaataaac cgtgtacacg cagcggttcg ataatcgaat caccgatggt ctgacgtggg
     4381 tccagcgaag cgtaagggtc ctgaaaaata aactgaatat cccggcgcaa tgcctgaagt
     4441 ttgccgggtg acaaggtatc gattcgctga ccgttaaaga taatttcgcc gccctgcgat
     4501 tcgaccaggc gcagcaacgc ccgcccggta gtggatttac cgctgccaga ctcgcccacc
     4561 agcgatagcg tttcgccagg ccagagatca aaactgactt tctcaacggc atgcacttcc
     4621 cgcgttacgc gattcaacaa accgctgcgc aaagggaaac gggtgaccag attacgcacc
     4681 cgtaaaacag gttcaccatc caccaccgtt ttctgctcga tggggggggc ctgtttcgct
     4741 ggatgttcaa gcgatatcaa tgggaaacgt cggggataat ctaacccttt catcgcacca
     4801 agttgcggaa cagcagctaa cagcgcacgg gtgtaaggat gttgcggtgc atgaaaaatc
     4861 tgttcgacgg tacccgtttc caccgcctcg ccctgataca tcaccagtac ccgatcggca
     4921 atctctgcca ccacgcccat atcgtgagtg ataaagataa cgcccatcga catctctttt
     4981 tgcaatactt tgattaattg caggatctgc gcctgaatag tgacatccag cgcggtggtt
     5041 ggctcatcgg caatcagcac cgccgggcgg catgacagcg ccatcgcaat catcactcgc
     5101 tggcgcatcc cgccagagag ttgatgcgga taacgtgaaa gaatggtttg tgcctcagga
     5161 atgcgtacct gatccagcat ccgcttcgcc tcgaccatcg cttcttcacg actggcgttc
     5221 tgatgcagac gaattgattc ggcaatctgt tcacccacag taaataccgg gttcagcgat
     5281 gtcatcggct cctgaaatat catcgccata tccgcaccgc gaacatggcg catttgtgca
     5341 gcgctctgct cgctaagttc aatcacttcg cgactgcgcc gctgcaacag cattttatcg
     5401 cactgtacta aaccgcccgc ctgttccaac aggcgcatca atgccaacgc agtcactgac
     5461 ttaccggagc cggattcgcc aacaattgcc agcgtctcac cgcgttgcag actaaaagag
     5521 agattgcgga ccgcagctat tttctgctgg tcctgcataa aggcaatatt cagattttca
     5581 accgccagca cattaccggc atcaagttca tcactgtgtg gcaacggtgt cccctttttc
     5641 acggtagata ccggtggttg gcgtatcgcc tgcgtagccc caggcgcgat acattccttc
     5701 ggtgttaaac ggtagcgcga cattcccttc atggtcgata gcgattaagc caccgctacc
     5761 gccaagcgca gggagttttt ccattactac ccgctcgcag gcttccgcga gacttaatcc
     5821 gccgtaatcc attaacgcgg cgatgtcata tgccgccagc gcgcggatga agacttcgcc
     5881 cgtgccggta caagaaaccg ccacactggc gttattggcg tagcatccgg cacccactaa
     5941 gggactatcg ccaactcgtc cgggtaattt attggtcatt ccgcctgtgg acgtggctgc
     6001 cgccaaattg ccgtctaaat ccaacgccac ggcccccacg gtgcccattt tctgtttttc
     6061 atccagtggc gcaccgctat ggtcgaggac ggttgcccct tccttgcgcg ctgccagtag
     6121 ttgttcataa cgcaaaggcg tggagaaaat ctccggcgag acgcgctcca tgccacgagc
     6181 aaacgcaaaa ttttctgccc cttcgccaat catcatcaca tgcgggcttt gctccatcac
     6241 cagccgggcg gcaagaaccg gattacgcag atgactaacg cccgccaccg caccggcttt
     6301 cagggtgtta ccatccatca cacaggcgtc cagttcatgg gtttcatcac gcgtaaagac
     6361 agcgccaatt ccggcgttaa acagtggaca ctcttccagc agacgcaccg cttccgtcac
     6421 cacatccagc gcactttcgc ccgcttccag cattttctgc ccggtttcaa caatggcaga
     6481 caacgcctca atgtagcgta attcctgttg cagactcatc tgcgcgcggc taattgcacc
     6541 tgcgccacca tgaattgcaa tgactg
//
LOCUS       DAZGXZ010000221         6469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000221 DAZGXZ010000000
VERSION     DAZGXZ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.222"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..815
                     /locus_tag="ACHKJF_005031"
     CDS             246..815
                     /locus_tag="ACHKJF_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0952241.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGEFKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1075..1476
                     /locus_tag="ACHKJF_005032"
     CDS             1075..1476
                     /locus_tag="ACHKJF_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0952242.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            1464..1766
                     /locus_tag="ACHKJF_005033"
                     /pseudo
     CDS             1464..1766
                     /locus_tag="ACHKJF_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            1900..2232
                     /locus_tag="ACHKJF_005034"
     CDS             1900..2232
                     /locus_tag="ACHKJF_005034"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0952243.1"
                     /translation="MELVRLALEEEGSIAALARKHDVNDNLLFKWIRLWQREGRVCRP
                     RKNSSSLPALIPVQLQAGSSLPTFELPSCSPPATCHIKCRGGEITLTHPSAELMSTVL
                     RELMRGPV"
     gene            2229..2576
                     /gene="tnpB"
                     /locus_tag="ACHKJF_005035"
     CDS             2229..2576
                     /gene="tnpB"
                     /locus_tag="ACHKJF_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0952244.1"
                     /translation="MINLPAGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLMEGIDW
                     RQPKRLLTSLTML"
     gene            2626..4161
                     /locus_tag="ACHKJF_005036"
     CDS             2626..4161
                     /locus_tag="ACHKJF_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP0952245.1"
                     /translation="MNDTSSDDILLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNLLQQESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPTEPCCPECGGSLSYLGEDAAEQLELMRSAFRVIRTVREKHACRRC
                     DRIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYARQGVVLSRSVLSG
                     WVDACCRLLAPLDEALQHYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGHIKEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIESELRGKRAEERQAVRHQKVLPLLASLEG
                     WLREKQKTLSRHSELAKAFGYALNQWPALTRYAEDGWVEVDNNIAENALRLVSLGRKN
                     WLFFGSDHGGERGASLYSLIGTCKLNGVDPERYLHHVLDVIADWPVNRVGALLPWRVT
                     LPA"
     gene            4200..4427
                     /locus_tag="ACHKJF_005037"
                     /pseudo
     CDS             4200..4427
                     /locus_tag="ACHKJF_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
     gene            4602..6221
                     /locus_tag="ACHKJF_005038"
                     /pseudo
     CDS             4602..6221
                     /locus_tag="ACHKJF_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            6301..6469
                     /locus_tag="ACHKJF_005039"
                     /pseudo
     CDS             6301..6469
                     /locus_tag="ACHKJF_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
BASE COUNT         1511 a         1701 c         1820 g         1437 t
ORIGIN      
        1 taatcgcgtt tccacagtcc cagcacttct ttttgcgtat caccataact ccctcctcac
       61 agccttaccc gtaaccggtt ttttacatta aaaaaacaac gctgaaaatt caaccgtcag
      121 tccggagacg accgttcggg ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca
      181 cttcagggac tctgcgcatt ttttacgacg tggctattcc gtatgaacca tacggagatt
      241 taaccatgac ctacaaatac aaccccttct ggcagcaacg tattcgtgag acggtgcggc
      301 acgcactgaa tgttcatccc cgcctgacgg cattgcgggt tgacctgcgt ctcccggatg
      361 taccggcagc aacggacgca gctgtgatat cccgcttcat caatgccctg aaagcccgaa
      421 tcgacgctta ccagaaacgt aagcatcggg aaggtaaacg cgtgcatccc accaccctgc
      481 attacgtctg ggcccgggag tttggggagt tcaaaggtaa aaaacactat cacctgatgc
      541 tgctggtcaa ccgggatacc tggtgtcgtg ccggtgatta ccgcgctccg gagtctctgg
      601 ccgggatgat taaacaggcg tggtgcagtg ccctgggagt ggatgtcggg tgccatgcca
      661 cgctggtgca ttttccggcc tggccggcgg tgtggctggc gcgtaatgat gacaccggct
      721 ttcagcaagt gctggaacgt gctgactatc tggcgaagga gcataccaaa gctcactgca
      781 ccggtgagcg caattttggc tgtagccgga gttgagcacg actggcgcgc caatatccgg
      841 caaaccaaaa gtatgggttg ttgtctggcg gcaacgatgt atccactcgc ccactgactg
      901 ttttttacgt cagtgttcca ttgtgtgaca accacagtgc tgtaacagca accagcctgc
      961 tttacccaaa ctccagtcca gattttaact gaaccgccat tcacaccctg accacgctgc
     1021 ccgtaccggg acggctgttg cctgcgtgtc gtctggcggc tgaggaatat gactatgtac
     1081 gcaaaatcct ttatcgctct tgatggcaac ggacgtctga cgggcgcccg tactgcacag
     1141 gccgcacctt atgctaacta cacctgccac ttgtgtggca gtgcactcag ataccatccg
     1201 caatacgaca ctgaacttcc ctggtttgaa cacactgacg acaggctgac agagcacggt
     1261 caacagtgcc cttatgtcag gccggagcgc agagaaatac agttgattaa acgtctgcag
     1321 caattcgtac cggatgcctt acccgtggtg cgtaaagcca gctggcactg cagacaatgt
     1381 caccacgatt attatgggga gcggtactgc acacactgcc agaccggagg attcagtatt
     1441 ccccggacaa ctcaggagga aatatgcgaa ttctgaactg ttatatggcg aatgacagca
     1501 aaggccattt tgttacggcg aaagaagcag cgaagcacaa ccgccaggac gttttgtgct
     1561 gtgtgtcctg tggatgcccg ttaacacttc agcggggcaa tgacggacaa ccaccgtggt
     1621 ttgaacatga ccagatgact gtcgctgaga aaatcctgct gcgatgcacc tggcttgacc
     1681 cggcagagaa agaggcccgt cgtttgcatc tgcagggtat gacggttccg gattatacgg
     1741 tgaaggtgag aaagtggttt tgtgtgtaag cgtcgtctga gtaccgtctg gtcccgaatc
     1801 caggatcctg taacttaagc acccacttat tttgcaggtg gacacctcat gcgcgctaaa
     1861 gaaagacttc cccggaaaca ctattcccct gaattcaaaa tggaactggt caggctggct
     1921 cttgaagaag aaggcagtat tgccgcactg gcccggaaac atgacgtcaa tgataacctg
     1981 ctctttaaat ggataaggct ctggcagcgt gaagggcggg tctgtcggcc ccgaaaaaac
     2041 tcatcgtcgc ttcctgccct gatacccgtg cagcttcagg cgggctcttc tctcccaacc
     2101 ttcgaactac catcctgctc tcctcccgct acctgccaca taaaatgtcg gggaggagaa
     2161 ataacactga ctcatccctc tgctgaactc atgagcactg tcctgcgcga actgatgcgg
     2221 gggcctgtat gataaatctt cccgcaggca caaaaatctg gctggttgcc ggtatcaccg
     2281 atatgcgcaa cggcttcaat ggcctcgccg caaaggtgca gaccgcgctg aaagatgacc
     2341 cgatgtccgg ccacgtcttc atcttccggg gacgcagcgg cagtcaggta aaactgctct
     2401 ggtccaccgg cgatggtctg tgcctgctga caaagcgact ggaacgtggt cgcttcgcct
     2461 ggccctcagc ccgcgatggc aaagtgttcc tgacgccggc gcaactggcg atgctgatgg
     2521 aaggtatcga ctggcgacag ccaaagcggt tactgacatc cctgaccatg ttgtagcgct
     2581 ctttatcctg gttgtcgcag aataagcctg gtaaaataca ggcttatgaa cgacacctct
     2641 tctgacgaca tccttctgct gaaacagcgc ctggccgaac aggaagcgtt gatccacgcc
     2701 ctgcaggaaa agctgagcaa ccgggaacgc gaaatagacc atctgcaggc gcaactggat
     2761 aagctgcgcc ggatgaactt cggcagtcgt tccgaaaagg tatcccgccg tatcgcgcaa
     2821 atggaagctg acctgaacct gctgcagcag gaaagcgata cgctgaccgg ccgggtggat
     2881 gacccggcag tgcagcgacc gctgcgtcag acccgcaccc gcaaaccgtt ccctgagtca
     2941 ctcccacggg atgagaagcg gctgctgccg actgagccgt gttgcccgga gtgcggtggt
     3001 tcgctgagtt acctgggtga ggatgccgcc gaacagctgg agctgatgcg cagcgccttc
     3061 cgggttatcc ggacagtccg tgaaaagcat gcctgccgtc ggtgcgatcg catcgttcag
     3121 gccccggctc cttcgcgccc catcgaacgg gggatcgccg gaccggggct gctggcccga
     3181 gtgctgacct caaagtatgc agagcacaca ccgctgtatc gccagtcgga gatctatgcc
     3241 cgccagggtg tagtgttgag tcgttctgta ctgtcgggct gggtggatgc gtgttgtcgt
     3301 ctgctggcac cgctggatga agcccttcag cactatgtcc tgaccgacgg caaactccat
     3361 gctgacgata cgcctgtccc ggtgctgttg ccaggcaata agaagacgaa gaccgggcgc
     3421 ttatggacgt acgttcgaga cgaccgcaac gccggatcag cgctggcccc cgcagtgtgg
     3481 ttcgcttaca gcccggacag aaaaggtatc caccctcaga cccatcttgc aggcttcagt
     3541 ggcgtactgc aggctgatgc ctacgccggg ttcaacgagc tctaccgcga cggccatata
     3601 aaggaagccg cgtgctgggc ccatgcccgc cgaaaaatcc acgatgttca cgtccgtacc
     3661 ccgtcagccc tgacggagga ggcgctgaaa cggatcggcg agctgtatgc catcgagtcg
     3721 gagctcagag gtaaaagagc agaggaacgg caggcagtcc ggcaccaaaa agtgctgccg
     3781 ctgctggcgt cactggaggg gtggctgcgg gagaaacaga aaaccctctc aaggcactca
     3841 gaactggcga aggcgttcgg gtatgcgctg aaccagtggc cggcgctgac ccgctatgca
     3901 gaagatggct gggtggaggt ggataataac attgccgaaa acgccctacg actggtcagt
     3961 ctggggagaa aaaactggct gttcttcggc tcggatcatg gtggtgagcg cggtgcgtca
     4021 ctgtacagtc ttatcgggac gtgcaaatta aacggcgtgg atccggaacg ctatctgcat
     4081 catgtacttg atgtcatcgc cgactggcct gtgaaccggg tgggtgctct gctcccgtgg
     4141 cgggttactc tgcctgcctg accagcttca cttcgtcaat acggttctca ctgcacgctt
     4201 acttttgtgt gatgtgtgac gaagattatg aaggagaaaa gtgctgcccg cgttgtggta
     4261 ccggggtata cagcagggag gggggctgca ggaaggcaac tggaaggata ggaactgagc
     4321 ggttcagtcg cggaaatcag ttgcagagtt gtaatgatat ctccgggagt gctgcgttgg
     4381 taggggcatc ccgggataga gaaattttag cactttgccc tgaatgattg tagacgttcc
     4441 gagtactcgt accggtctga gttattacag ttagatattc actttttctc ccgcatactt
     4501 cacatctgtc aacgacggat gaaaagtggt tcttccgcaa cctgggtgga agaaaatgga
     4561 tgaaactttc tggcgaacaa ggatctaagc tgttttaagt tatgggcaac gcaatgcact
     4621 ctcttaagac acttctacag ttaccttgcg gatggcgatg cagtcgacaa attattagct
     4681 ctgacggtat aaccctccat ctccacggaa aacgcaaaac agcacaatgt cctgaatgct
     4741 ttaagcgtag cgactctgtt catagttgtc gtcggcgccg gatacagcat ctaccctgct
     4801 cagggcagac gctatggctt gtattttccg tccgccactg gtactgccgt aacccggctt
     4861 gttcacgtaa aatttttgcc gagtcgcttg ctcccttcgc cggttcacac cagcagtctt
     4921 cacaggcgtt acaaaattta caacgtcaac tgggattaat agctggaggt gaggctggaa
     4981 aacgggctgc aacggcagcg ggtctccgtt gcagtgctga tactcttctt cgcagggtta
     5041 tcaatacccc ggagacgaaa cagtcaggcg cgcctcatgt cggtattgat gagtgggcgt
     5101 ggcatcgggg ccactgctgc ggtatgttaa tagtcaatct tgatactcac cgtcccctcg
     5161 tcctgcttcc cggccgtgat cagcgtacgc tggcgacctg gttcagaaaa tatccggaaa
     5221 tacaggttgt ctcgcgtgat cgcagtggag tctatgcgac agcagcacgt gaaggtgcac
     5281 ctcaggccag acaggtggcc gatcgatggc acctgctaaa aaatattggt gatgagcctg
     5341 aacgaatgat gtacagacat atgcctctga tacgtcttgt tgtcagagag ttatcactga
     5401 agaaatcacc tgagccagaa atatctgtgc ctgtagcatc gctccgtcgt ctggaacgcc
     5461 ttaaacagca catccgcaaa aaacggcatc agcgttggac agaggttatg gccctgcata
     5521 acaagggatg tagtttcagg gaaatatccc gtattacagg cctgtcgcga gtgacagtca
     5581 gtcgctgggt gggttcagga acattccctg aaatgtcaac taggcctcca aagcgagggc
     5641 ttctggaccc atggagggag tggttaaaag agcaacgaga atgtggtaat tataactccg
     5701 gccggatatg gcgggaaatg gtggccaggg gggttacagg cagtgaaacc atcgtcaggg
     5761 atgctgttgc caaatggcgt aaaggctgga tcccaccggt tactactgcc gcaagacttc
     5821 cttcagtgtc ccgggtaagc cgctggttga tgccctggag aataatcagg ggtgaagaaa
     5881 attatgcttt ccgatttatt agtctgatgt gtgaaaaaga accggagttg aaaatagcgc
     5941 agcaactggt actcgagttc tatcgtattc tgaaaaccta aaataaatca cagctaagca
     6001 gctggttcac tcgggtccac gaaagcggct cagcagaact tcggtgcgta gctgcgggga
     6061 tggaagctga tgctgcggct atatgtgagg caatcagcag tcgctggagt actggtgttg
     6121 tcgaaggtca tgtaaatcgc ctgaaggtgc tgatacgcca gatgtatggt cgagccggac
     6181 ttgaactact taggcggagg gtaatgagtc cactggcatg aaaatcttca cccaggttgc
     6241 ggaagagcca cttttgcact gttcgtgaca gtatggcctt tatgtactca ctttgttacc
     6301 ggtagctgga tcatcactac cgtgctgcaa atggtggtga acagcatcaa ttgtggaatg
     6361 aatgttgccg aagcgaccaa tgcgcctcgt ttccactatc agtggttgcc ggacgagctg
     6421 cgtgttgaaa agggtttagt ccggatacgc tcaagctgct ggatgcaaa
//
LOCUS       DAZGXZ010000222         6404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000222 DAZGXZ010000000
VERSION     DAZGXZ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.223"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..142
                     /locus_tag="ACHKJF_005040"
     CDS             <1..142
                     /locus_tag="ACHKJF_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0952246.1"
                     /translation="NLGNNESLVCGVFPNQDGTFTAMTYTKSKKFKTEAGARRWLARN
                     SD"
     gene            complement(256..771)
                     /locus_tag="ACHKJF_005041"
     CDS             complement(256..771)
                     /locus_tag="ACHKJF_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952247.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQHECVSLVELVSTYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVTVN
                     GGSIIMHLCPKCFGQKIVSILQPFPVREYEFFHKSRCLYCENQFLMNKNPDYVSPPSI
                     EELARKLNGNL"
     gene            1300..1488
                     /gene="dicB"
                     /locus_tag="ACHKJF_005042"
     CDS             1300..1488
                     /gene="dicB"
                     /locus_tag="ACHKJF_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0952248.1"
                     /translation="METSLPNVNTSEGCFNIGILLSNREFTEDAIRMRKYEPYLLNDN
                     SILSRIALLELGIFGGQQ"
     gene            1485..1673
                     /locus_tag="ACHKJF_005043"
     CDS             1485..1673
                     /locus_tag="ACHKJF_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0952249.1"
                     /translation="MSSAFALMMTVFLITGEPQNVITGIYASKESCLQARDEQKISGE
                     CLPLNKVSLYLNNETPAG"
     gene            1769..4240
                     /locus_tag="ACHKJF_005044"
     CDS             1769..4240
                     /locus_tag="ACHKJF_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0952250.1"
                     /translation="MSKIFICAAIPDEQAIKNEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGTHRPALDSWDAEYMLENRWDEESASFVPVETESDPM
                     NVTFDKLSPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPEIKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLART
                     RVNQHLTENSAAKINPVTTAIRREYRQTWKTLDKELACALWPGDVDAGNIDGTIHRWA
                     KNEVIDKDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIYKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADAILSSAAQTDPVETAESNSQKNEILVEAEPSVEREGPFYF
                     VFTDKDGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLQQNTDTAEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDTDKLLAASRGEFVEGISDPNDPKWVKGIETRDSV
                     NQNQPESEQNDQKAEQNSPNALQNEPETKLPEPEVQQEPEKVCTACGQTGDGNCPDCG
                     AVMGDATYQETFDEKNQAEVQEDDSEEMEGAEHPNNENAGNDQHHTSDSETGEAAEPL
                     IAENGHHIITSTSRVWIHLSVDLETMGTNPDAPINSIGGKFFDPATGEMGPEFSKAID
                     LETSGGIIDRKTIKWWAKRSREAQSAIFTDEISLDVALRLFIEFIEKNSGGRFVQVWG
                     NGANFDNVILRRSYERQGIPCPWLYYNDRDVRTIVELGNAIGFDVRMAIPFEGVPHNA
                     LDDARHQAKQVSAIWQKLIPSQADF"
     gene            4305..4553
                     /locus_tag="ACHKJF_005045"
     CDS             4305..4553
                     /locus_tag="ACHKJF_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0952251.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            4531..5661
                     /locus_tag="ACHKJF_005046"
     CDS             4531..5661
                     /locus_tag="ACHKJF_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0952252.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVSPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTQILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(5707..6345)
                     /gene="ompW"
                     /locus_tag="ACHKJF_005047"
     CDS             complement(5707..6345)
                     /gene="ompW"
                     /locus_tag="ACHKJF_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP0952253.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
BASE COUNT         1891 a         1487 c         1519 g         1507 t
ORIGIN      
        1 caatcttggc aacaacgaat ctctggtatg cggtgtgttc cctaaccagg acggcacgtt
       61 taccgcgatg acgtatacca aaagtaaaaa gtttaaaacc gaagctggcg cgcgtcgctg
      121 gttagcaaga aactctgact aatgaagtct ggtagttaag gaatcctcca cggggagaac
      181 tggtgcacac gcgccggaca caagcaagca tccggcatgc tctttaacaa tctggatatc
      241 cataacagta acaatctaca gattgccgtt cagttttctg gccaactcct caatagatgg
      301 aggcgataca taatccggat ttttattcat aagaaactga ttttcacagt agaggcacct
      361 gcttttatga aaaaactcat attctctaac cgggaatggt tgaagtatcg atactatctt
      421 ttgtccaaaa cattttggac aaagatgcat gattatgctg ccgccgttca cggttacctc
      481 cttcgagtac acaaaagttc ccgactcaag ttggttaagg atataacctt ccgtctgagc
      541 ctcaaagttt tcaaattctg caattttagc tttgagagaa gcatttattt cttgataagt
      601 gctcaccagc tcaacgagag acacgcattc gtgctgaata gatgcaagct ttgagttcag
      661 ttcaccaata gccgcattta cttcagcttg agtttttgcc tcgttcatta gttttgcaat
      721 ctgagcggtt tcacgaatag ccgtcattgc tgccgttaat tcagcgatca cattcaatac
      781 tcttattgtt gttggggata tccagattaa caagatcctt gttgttgggg aataacaggt
      841 ccacctcgcc tgacgtggtt aaaagcaggc acacaacacg aaagcgctcg gcgaagttag
      901 tctctctgta tatgtcgtcg ttaaatgtaa ttcgatcgtg cgcttccggt tgtggcaatc
      961 cgcgaaatgg cgcggcggta agtatggctg gggcttcctc cattgctcca gaaaatgcac
     1021 cgggttgtca ggttgaccat acacctgagt gacaacaccg ccacaacaac ctctgttatc
     1081 acttttctgg tgattcggcg gaaatggata tccgccattt ttaaagtgta ttttgtgatg
     1141 cggtgaatgc ggctaagcgc acgcggaaca gttaaaacaa gcggtctttt actggcgtaa
     1201 cagacatcaa ctaacaatcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
     1261 aggcaccaca tcacaaaatt cattgttgag gacgcgataa tggaaacgtc actaccaaac
     1321 gttaatacgt ctgaagggtg ttttaatatt ggtattctgc tcagtaaccg ggagtttact
     1381 gaggacgcca tcaggatgag aaaatatgag ccttatcttc tcaatgataa ttccatactc
     1441 tccagaattg cccttcttga acttggcatt ttcggagggc agcagtgagt tcagcgtttg
     1501 cactgatgat gacggttttt cttataacag gtgagccaca gaatgtgatt accggaattt
     1561 atgccagtaa agaatcctgc ctccaggcaa gagacgagca aaaaatttct ggtgaatgcc
     1621 tcccgttaaa caaagtatcg ctgtacctga ataacgaaac accggctgga taacccacca
     1681 gccatattaa cgccatacca acggattaaa aatgccagca atggcaggga ttcgttcacc
     1741 ctgaaatctg taatgaggtt taaacacaat gagtaaaata tttatttgcg ccgccattcc
     1801 tgacgaacag gcaataaaaa acgagggcgc tgttgctgtg gccactgcca ttgaagccgg
     1861 tgatgaacgt cgcgcccgcg caaaatttca ctggcaattc cttgaacatt atccggctgc
     1921 tcaggactgc gcttataaat ttcttgtctg tgaggataaa cccggtacac accgcccagc
     1981 cctcgactcc tgggatgctg aatatatgct ggaaaaccgc tgggatgagg agtctgcttc
     2041 ctttgtcccg gttgagactg aatcagatcc gatgaacgtc acttttgaca agctgtcccc
     2101 tgaagtacag aacgctgtca tggttaagtt cgacacatgt gaaaacatca ccgttgatat
     2161 ggttattagc gcacaggaat tgttgcagga agacatggca accttcgacg gacatatcgt
     2221 tgaagcgttg atgaaaatgc cagaagttaa cgccatgtat ccggagatta aactgcatgc
     2281 catcgggtgg gttaagcata aatgtaagcc tggtgccaaa tggccagaaa ttcaggcaga
     2341 gatgcgcatc tggaaaaaac gtcgcgaagg tgaacgcaag gaaaccggaa aatatacgtc
     2401 tgttgttgat ctcgcacgca ccagagttaa ccagcacctc accgaaaact cagcagcaaa
     2461 aatcaaccct gtcactacag ccattcgtcg cgaatacagg cagacatgga aaacactgga
     2521 taaagaactg gcctgcgccc tctggcccgg ggatgtggat gcaggaaaca ttgacggtac
     2581 catccatcgc tgggctaaaa atgaggttat cgacaaggat cgcgaagact ggaagcgtat
     2641 ctcagcatca atgcgcaaac agcctgatgc gcttcgctat gaccgtcaga ctattttcgg
     2701 ccttgttcgt gagcgcccga tcgacattta caaagatccc gttgcactga acaaatacat
     2761 cagcgaatac ctaacgacaa agggcgtgtt tgaacatgaa gaaacagacc agagctctgc
     2821 tgatgctatc ctgtcgtcag cagcacaaac tgatccagtg gagacggcgg aatccaattc
     2881 tcaaaaaaat gaaatcctgg tggaagctga accatctgta gagcgtgaag gaccgtttta
     2941 tttcgtcttt accgataagg acggggaaaa atacggcagg gcaaacaaac tttctggcct
     3001 ggacaaggcg ctggctgccg gtggtaccga aatctcaaaa gaagaatatt ttgcccgaaa
     3061 aaatggcaca tatacgggct tacagcaaaa tacagatacc gcagaagatt cagaacaacc
     3121 agagccggta aaagttaccg ctgacgaagt aaacaaaatt atgcaggcag ccaatatcag
     3181 ccagcctgac accgataaat tgcttgctgc atcacgtggt gaatttgttg aagggattag
     3241 cgacccgaat gatccgaaat gggttaaggg gattgaaacc cgcgattctg tgaaccagaa
     3301 ccagcccgaa tcggaacaaa acgaccagaa agcggaacaa aacagtccaa atgcgttaca
     3361 aaacgagcca gaaacgaaac tgcctgaacc agaagtgcaa caagaaccgg aaaaagtctg
     3421 caccgcctgc ggtcagaccg gcgacggcaa ctgtcctgac tgtggcgcgg taatgggcga
     3481 cgcaacatac caggaaacat tcgatgaaaa aaatcaggct gaagttcagg aagatgattc
     3541 ggaggaaatg gaaggcgctg aacatccgaa caatgagaat gctggtaacg atcagcatca
     3601 caccagcgat agtgaaactg gcgaggcagc agagccctta attgcggaga acggtcatca
     3661 cattatcaca tccaccagca gagtgtggat tcacctgtcg gtcgaccttg aaacgatggg
     3721 aacaaatcct gatgcgccaa tcaactctat tggcggtaaa ttttttgatc cggcaaccgg
     3781 agagatgggg cctgaattca gtaaagctat cgatctggaa acctccggcg gaatcatcga
     3841 ccgtaaaaca ataaagtggt gggcgaaacg atcacgcgaa gcacaatccg ctatttttac
     3901 cgatgaaatc tcgttagatg ttgctctccg gctattcata gaatttatcg aaaaaaactc
     3961 cggcgggcgt tttgtccagg tatggggtaa tggagccaac ttcgacaacg taattttacg
     4021 ccgttcatat gaacggcagg ggatcccctg cccgtggctt tactacaacg atcgcgatgt
     4081 acgcacaatc gttgaactgg gtaatgccat tggctttgat gtcagaatgg ctattccatt
     4141 cgaaggcgta ccccacaatg cgctggacga tgcccgtcac caggcaaaac aagtttcagc
     4201 aatctggcaa aaactgatcc cgagtcaggc tgatttttaa tgttcaaccc cggtcgtcgt
     4261 ccgcaagcta tagtggcggc ggccatgatt agcgaacaac gctcatggca agacttattc
     4321 tgctcactga gtgggcaaaa gaggaattca gtgaaccggt ccctactccg ggcacgttaa
     4381 gtaaatacgc taaagccgga atgatatttc ctctccccaa aaaagttgga agacactggc
     4441 gagtggatcc tcgagctcgc tttgtcggaa tggtaaacaa gccggaagtg atcgccacag
     4501 atcaccctgc tttgaagagg atactggaag atggcgcgcc cacgaaaata taaaactgaa
     4561 gttccgggat tatctccgta ttttgacaaa agaaataaca aagtttactg gcgttacagg
     4621 catcccataa caggaaaaaa tcacgggctc ggcagtattg accagaaaca ggcagaagct
     4681 attgcagcag aagcgaacag ccgtcttgcc aggcaacaaa tggaacaaat gctcactctg
     4741 caggagaaaa ttattcgtga taccggtggt tcatcaaccg tttctgtttt tctgaatagt
     4801 taccgaaaaa ttcaacagga aagatatgaa aacggagaga tcaaactcaa cacactgaaa
     4861 cagaaagcat cccctctcag ggtatttgat gaacgttttg gtaccagacc attagatgcc
     4921 ataaccgtaa aagatgtggt atcggtgctg gaagattaca aggcaaaagg acataacaga
     4981 atgggacaaa ttttcaggaa agtactgatt gatgttttcc gggaggccca gcaaacgggc
     5041 gatgtctcgc caggctttaa ccctgcagaa tcggcaaaaa aaccacaggt acggatatca
     5101 cggcaacgac tgacctttga tgagtggatg atgatttata atgcagcgga aaaggatggt
     5161 tactttttac aacgtggtat gctgctggcg ctgatgacag gccagcgcct ttcagatatt
     5221 tgcaaaatga aattttcgga tatcagggat ggttatcttc atgtcgaaca gcaaaaaaca
     5281 ggaacccgga ttgccatccc tctggctctg cgttgcgata aattaaatct caccctggat
     5341 gatgtagtgt catcctgtcg cgattgcgtt cttagtccgt ggttattgca ccaccatcat
     5401 gctaaaggga cagctaagcg cggcgggatg gttaagccag caacgttgac agttgcattt
     5461 aaaaaagccc gggattctgt ggattacaac tggcgcgcta atggcactcc cccctctttc
     5521 catgagcaga gatctttatc agagcgattg ttcagggaac agggaattga tacccaaatt
     5581 ttgctgggtc attcgaatca aaaaatgacc gatatttaca acgatgtacg cggtaaagag
     5641 tggaaaaaac tggtcatttg atgaccagtt ttgcagagga gttttgcaga gattttgcag
     5701 agaaaattaa aagcgatatc cagcagagaa cataaacacc cacggatcca gtcgtaccga
     5761 gtctttcacg gtagtaacac cagatttata cttggccgtg gtatcgatat ccatgtacca
     5821 cactgacatg ttaaccaacc agtcacggtt aatcagataa tcaaccccca cctgcccggc
     5881 agctccccag gaatctttca gactgagatc ggaaagcccc gcctctttgc catgatcgtt
     5941 aaatccatta tcaaagaagg tggtgtagtt aataccggcc ccaacgtaag gacggaattt
     6001 gctgctggca tcaccaaaat accactgcgc catcagtgtt ggcggcagat ggtgaacggt
     6061 tgcaatatcg ccggtcgccc gggtgccgat tttatggcgg aacggcgtcg ctgccagtaa
     6121 ttccacacca atgttgtcgg tcgccatata agtaaacgtc aagcccagtt gagtgttatt
     6181 ggtcacgctg aatccaccca gacttcctaa cgtaccacca gcaccttctg ttggacgtac
     6241 ggttgcagaa cctgcacgca taaaaaattc gcctgcttca tgcgcaaagg cactgccaga
     6301 aagaagagtt gttactgcca aagccgccac tgttaacttt ttcatatccg ctccgtcgtt
     6361 atggttataa aaacgtgacg aatataccta cattggagta acaa
//
LOCUS       DAZGXZ010000223         6369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000223 DAZGXZ010000000
VERSION     DAZGXZ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.224"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..498)
                     /locus_tag="ACHKJF_005048"
     CDS             complement(<1..498)
                     /locus_tag="ACHKJF_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952254.1"
                     /translation="MNKTLNALICRHARNLLLAQGWPEETDIEQLNPHYPGWISIYVR
                     LDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEEEIRAVLDAVRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESD"
     gene            complement(543..1095)
                     /locus_tag="ACHKJF_005049"
                     /pseudo
     CDS             complement(543..1095)
                     /locus_tag="ACHKJF_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            complement(989..1210)
                     /locus_tag="ACHKJF_005050"
     CDS             complement(989..1210)
                     /locus_tag="ACHKJF_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952255.1"
                     /translation="MNYAGHEKLRAEVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LARQTLYRANRLFMEVYTEILELDSCFKD"
     gene            complement(1211..1894)
                     /locus_tag="ACHKJF_005051"
     CDS             complement(1211..1894)
                     /locus_tag="ACHKJF_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP0952256.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFVAAWKNAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(1970..2281)
                     /locus_tag="ACHKJF_005052"
     CDS             complement(1970..2281)
                     /locus_tag="ACHKJF_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952257.1"
                     /translation="MKYDGEGRATARFFSGKGCRHAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRT
                     R"
     gene            complement(2278..3180)
                     /locus_tag="ACHKJF_005053"
     CDS             complement(2278..3180)
                     /locus_tag="ACHKJF_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0952258.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTKDVRFEQCHGLTAAGRGVVSPENIAFTRWLTYLQDGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGIPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIKVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            3619..4578
                     /locus_tag="ACHKJF_005054"
     CDS             3619..4578
                     /locus_tag="ACHKJF_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0952259.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDGKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLRTLINDVAEIKNITHVIDSEVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            4578..4973
                     /locus_tag="ACHKJF_005055"
     CDS             4578..4973
                     /locus_tag="ACHKJF_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0952260.1"
                     /translation="MESSDPKKRKKVVAYLHPTLYPQDNLTQQTIDSLPIQMRGDFYR
                     QSLICGAALYSVDPRLLTLISGFFSEKITAENLVKLIEQTTGYTSTSIDINVLKNIIE
                     ASSENKSESIASKDDFEEQTRRNLSMLKK"
     gene            complement(5088..5420)
                     /gene="pemK-mt"
                     /locus_tag="ACHKJF_005056"
     CDS             complement(5088..5420)
                     /gene="pemK-mt"
                     /locus_tag="ACHKJF_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK-mt"
                     /protein_id="HFP0952261.1"
                     /translation="MERGEIWLVSLDPTAGHEQQGTRPVLIVTPAAFNRVTRLPVVVP
                     VTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRTIDMKARGGKRLERVPETIMNEVL
                     GRLSTILT"
     gene            complement(5422..5679)
                     /gene="pemI"
                     /locus_tag="ACHKJF_005057"
     CDS             complement(5422..5679)
                     /gene="pemI"
                     /locus_tag="ACHKJF_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HFP0952262.1"
                     /translation="MHTTRLKKVGGSVMLTIPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPHRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(5772..>6369)
                     /locus_tag="ACHKJF_005058"
     CDS             complement(5772..>6369)
                     /locus_tag="ACHKJF_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP0952263.1"
                     /translation="GISWGARFVMELAVLLYGPGKNYLFFSHGAVLMYLLCVFLVYRR
                     WIAPLPVVGQLRNVGVLWLVGAMAVVYIGVFLLGKVLALPAEPFMTKLFADKSIPDVI
                     LTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGALITSLLFVAAHSQYKNLLTL
                     AELFLVGLITSAARIRSGGLLLPVLLHMEATTLGLLFG"
BASE COUNT         1688 a         1748 c         1559 g         1374 t
ORIGIN      
        1 atcgctttcc ttcacaacca gacgaaagcg gcgcgtctgg cgggtcagca acgtctgacc
       61 ggtggtggtc agcgccgcac ggatacgccg cgcatcttct gccacccggc aactgacact
      121 gcatactgca tcgcgcaccg catcaagaac cgccctgatt tcttcctccg tcagccattc
      181 accggcatac gggaaagaca cctgcgttcc gtctgccgga agtaccggca gcgactgcca
      241 ctgactgccg gacagaacaa gttccgctcc ggttccggtc agtttctgaa tggcagaggc
      301 caggagcggc ggcagtacgc cgccgtgacg gttgataagt aacgtcgcca gccggggcgc
      361 atccagccgc acgtaaatgc tgatccagcc cggatagtgc ggattcagtt gttcaatatc
      421 cgtctcttcc ggccagccct gcgccagcag caggttacgg gcgtggcgac aaatcagtgc
      481 attcagcgtt ttgttcatga ttaatggtct ccctgaatcg ccgcccgaaa gcggcgataa
      541 catcagtcgt tttcgtgtgt gttgtttttc agcagggcgc gggcctgatt aaccaccgcc
      601 tgacggcggg tgacgcattc cgggacgaag aggccgttat cacgggcggc ccattcacgc
      661 ttccacaatg cctgttcagc ggtctgaatc agggacgcca gcagttcagc agggacggtc
      721 acctggcggt tttctctcac gttattgatg atctccatcg ccacgccgga agaaataccg
      781 gattcagtcc gctggtcttc cgggatgtcc tccaggcgcg ccaggacggt tctgatttca
      841 tgatcggaca gggcaatatc cattccgtca gcgagggcct gaatttcttc cggggaccag
      901 ataaccagca tcagtggcgc gtctcccgga tactttgctg ccagctcccg gcatagcgtt
      961 tcgcatgttc cgtacatact ctctcccctc aatctttgaa gcaggaatcc agttccagaa
     1021 tttcggtata tacctccatg aacaggcgat tggcgcgata cagcgtctgg cgtgccaggc
     1081 gttcaacaag cacatcggaa tcaaagcgac agcgatgttc catatcgttc agcctggccc
     1141 gaaggtcaca catggtattg gccacctctg ccacttcggc gcgcagtttt tcgtgtccgg
     1201 catagttcat ttacgcagcc tccggttcaa accagttatc attcgcggcc ccctgctgca
     1261 tggcccgttg cacggcggcc agtcgttgct gtccggcacg gtgatactgt tcaagcagct
     1321 cgataccgat ataccggcgt ccggactgaa gggcggccac gcaggttgag ccgctgcctg
     1381 caaacgggtc cagcacaatc gcgttcggat gtgtgaagct ctcaatcagt ggttgcaggc
     1441 tggtaacggg cttttccgtc gggtgatgac gattgcctga atatttccag cccagcacat
     1501 ccggcagcgg gttacggggc cgggccggac tgccctttgc caggatgtaa gcgcattcgt
     1561 ggcggtacgc gacatacgca gatttggatg tgtagttttt ggtgaacacg aggtgcccaa
     1621 ccacgctgaa tcccgcattt ttccaggcgg ccacaaagcg atcgacgcgg ttccagccat
     1681 agaagctgac catcagcgca tcttttttga gtacgcggta catttcatta caggccggtt
     1741 gcagccattc atcggtttta tcgccagcga tggtgcgccc ggaacggtca cggaaaccga
     1801 cgaggtacgg cgggtcggtg aggatgaaat caatggcgtt atcagggata cgggccataa
     1861 catcgatgca gttaccgagg acaaaacggg acatacaaaa ctccttctct tacagagggc
     1921 accgggaggg cacccctgtg gtgcccgttc cccgctgcct gtgggctgtt caccgcgtcc
     1981 ggtaacgggt caggccgacc gtggaatacc gcagccgtcg tctgtttgac ggcaaggtta
     2041 tgccgcgaaa gcggcttgaa gggcgttcat cagaaaaggg cgacaggctg gcgtacactg
     2101 tcaccgacgc cagccgggag cggtaaaagc gcccgtcccg tgcccgccgg acgcgggaca
     2161 ctgaccacag gcagcgttta tgccgcgccg cgtcagccgg cgcagtaaaa agaggagcat
     2221 ggcgacatcc tttaccggaa aagaaacggg cagttgcccg cccctctccg tcatacttca
     2281 tccaccgtaa tgcgccacct tgtcgactat ccacgcaggt ccggtgactt ccggcagctc
     2341 atcgtcatct gtcggggaag accattccag ttccccccac agcacatcaa caagctctcc
     2401 acgctcatat cgctgccagc cacagaaatt gcagccctgt tcggcgtacc agtgctccag
     2461 cgtgcagctg aaacggcggc ttaattctgc aataacgtcg ctttccggct gacaccacgg
     2521 cgtgtcgaaa tccaccttaa tgaagttgtc tccctgactg ctgatattca cctcataccc
     2581 cacaggccac ttcacgccat actgttccgt ataccagtga tatgcagaag gaataccgtt
     2641 cagaacacca ccgttaaagc cattcacttc aacatccagg cgggtgggca gaaccgtcag
     2701 caggtcaaac ggcatggttt tttccggcag tacgttgtca cacagacggt tccaccacac
     2761 cgatacatcc tcgttactcc agaagccaca ccagtcacct ctttttgcgg tgaaaagtgc
     2821 ggtgatggta tccctgacat cgtccggtaa tccttcccag cgacgctgtc cagtaccgct
     2881 ctgcagccag agttcatgca gcatcaggca gttttgctca tccagcagta caccgtcctg
     2941 caggtatgtc agccagcggg tgaacgcgat attctccggc gatacaacgc cacgtccggc
     3001 agccgtcagt ccgtggcatt gttcaaagcg cacatcttta gtggtctgca gaagtccggc
     3061 acttccggcc agaaacagct gaataccttc atttgtggcg cggcgataaa acggtgtgac
     3121 ggcaccactg gccagtcgtt taatctcagc gatctgtgcc ggttcaccag aaaaatgcat
     3181 acggttactg caccagtttg gcatgggaat tctcctctca catgcggcct ccatcaggcc
     3241 gtgccgcacc tgtaaccggg cgaccgcata cgaatgcagg tgcggccccg aagccgcagc
     3301 gcgtggagga ggaacgacga aacgtgaccg ggtgaggagg ccggcgacag cgacacactt
     3361 acatcggatg cagcccggtt aacgtgccgg cacggcctgg ttaagtagtt tccgtctcag
     3421 ttcgtgacgc aaaatgagaa accaataaga actcaaaacg agtcacaaca agagcataaa
     3481 gagactccaa aagagcaacg cggcttgaca tagactcaaa atgagacaca ataagaaaca
     3541 acaaaaactc aaaaagatgc actgacataa aagatgctca atttgagaca aatacaaact
     3601 caaaacgagg caaaaagaat gctaaaagta tcatgcgatg atggttcaac taacgtaaaa
     3661 ctggcctggc ttgaagatgg cgaggtaaga accagtctgt caggaaacag ctttaaagaa
     3721 ggctggaacc cgggactgtt taatgccggc aaagtttaca attacgtcgt ggatggaaaa
     3781 aaatacacct atgatttggg cagcactgct gtcataggta caacacatgt cagttatcaa
     3841 tacagcacca ccaatctgct tgccatacat catgcactgc tgacatccgg ccttcagcca
     3901 caggatgtag aactgacggt aacattacct gtgaccgaat tctttgataa tgacaatcaa
     3961 ccaaatgaag aaagaataga aagaaaaaag gcaaatgttc tccgtgagat atctctgaat
     4021 aaaggggaaa catttaaaat taaaaaagtt aatgtaatgc ctgaatcttt gccggctgca
     4081 tttgaatcac taaaaaaaga taaagtaaac aaacttgaac gcagcctgat tatcgatctt
     4141 ggaggaacca ctctggattg tggtttaatt ctcggtgcat ttgaaggcat ttctgaaatc
     4201 cgtggctatt cagaaatcgg aacatcccgc ataactcata ccgtcatgaa tgcactaaca
     4261 aaagcctcca caccctgcaa ttattttatt gctgatgagt taataaaaaa caggcatgat
     4321 aatgaatatc tccggacatt aataaatgat gtggctgaaa taaaaaatat tactcatgta
     4381 atagacagtg aggtgaaatc tctggcagaa agcatccggc aggaaatttc aacattctca
     4441 ggaatgaacc gaatctacct cacaggcggg ggagccgagc ttatttaccc tcacataaag
     4501 caatatttcc cgaatctgaa agtgaacaag gtggatgaac ctcagtttgc attggtgaaa
     4561 gcaatggtgc atgcgtaatg gaaagcagtg atccgaaaaa aagaaagaaa gttgtggcat
     4621 acctgcaccc gacactttat ccgcaggata acctgacaca acaaacaata gactctctcc
     4681 cgatacaaat gcggggagac ttctacagac aatctcttat ttgcggagca gctctttatt
     4741 ctgttgaccc ccgactgtta acgctaatca gtggtttttt cagtgagaaa atcactgcag
     4801 aaaatctggt aaaacttata gagcaaacaa caggatacac atccacatca attgatataa
     4861 atgtgttaaa aaacattata gaggcatctt cagaaaacaa atcagagagc atcgcttcaa
     4921 aagatgattt cgaagaacaa accagacgca atctgtctat gttaaaaaaa taataaaaga
     4981 agagcaggtt cagttattac ctgctcttct tttataatga gggaccagca gcggttttag
     5041 tgccctaaat cgaacgtttt cgtccggttg cccctcaaac cccatgttca agttagaata
     5101 gtggacagac ggccaagaac ttcgttcatg atagtctctg gaacccgttc gagtcgtttt
     5161 ccgccccggg ctttcatatc aattgtccgg ggttgatcgc aacgcacaac acctgtggta
     5221 cgtatgccaa caccatccag cgacaccgca aagccggcag tgcgggcaaa attgcctccg
     5281 ctggttacgg gcacaacaac aggcagtcgg gtcacgcgat taaaggccgc cggtgtgaca
     5341 atcagcaccg gccgcgttcc ctgctgctca tgacctgcgg taggatcaag cgagacaagc
     5401 cagatttccc ctctttccat gtcagatttc ctcctgacca gtcgccggtg catccagcca
     5461 ttctcgttct tcagctgata tttcagcatt cggatcacac tgtgccagta gctcagccag
     5521 tgaatattgc gggcgtctgt gcggctcaac aatcagccgg ccattatcaa tgaccatgcc
     5581 aacttcatta tctgtaccca gagacagcgc attcagcagt gccggtggaa tggtcagcat
     5641 aactgagccg ccaaccttct tcagtcgggt ggtatgcatt cttcacctcc ataaaagtta
     5701 tatttaaata taacatccac taaaaaaaca caccaggctt taacgcgtaa tgtttaataa
     5761 aaatataact ttcaaccaaa cagtaaaccc agagtggttg cttccatatg cagcaatacc
     5821 ggcagcagca ggccaccact tctgatcctg gccgctgatg taatcaaccc caccaggaac
     5881 agctctgcca gtgtcagcag gttcttatac tggctgtgcg cggcgacgaa caacaacgac
     5941 gttatcagcg cccccagcca catcgtccag cagtaccgtg aacggaagac gttcagcata
     6001 atcccccgga atagcgtttc ctcattcaac ggggcaagga taaagatggt cagcaacgtc
     6061 aggatcacgt caggtatgga cttatcggca aaaagtttcg tcataaatgg ctcagcaggc
     6121 agagccagca ccttaccgag cagaaataca ccgatataca ccacggccat cgcaccgacc
     6181 agccacagta cgccaacgtt gcgcagctga ccaacgaccg gtagcggagc tatccaacgg
     6241 cggtatacca ggaaaacaca cagcaggtac atcagaacag caccatgact gaagaacaaa
     6301 tagttttttc ctggcccata aagcagaacg gccagctcca tgacaaatct ggctccccag
     6361 ctaatgccc
//
LOCUS       DAZGXZ010000224         6335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000224 DAZGXZ010000000
VERSION     DAZGXZ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.225"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1883)
                     /locus_tag="ACHKJF_005059"
     CDS             complement(<1..1883)
                     /locus_tag="ACHKJF_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0952264.1"
                     /translation="MNLRKKHICIAISLVLNPASISQASAQADTNKLTVEVDETSLGH
                     DVYGDDWKSIEIGTTKFTAKDESKGINFDHKSPVAEGRNSIAIGTSAEAGGDISTKEG
                     LYTGESIAIGAKTKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGKTYGREKIENN
                     YLASEAKGDGSIALGSRTFAKGELSVSLGSVSSAEGMASNAIGATAEAKGDMAIAVGF
                     NALANAGNALAVGSQSSANKNDALAVGSEANAAQNETVALGHGATVTVAKGNVSIGAD
                     SRDKQAQKVTEAKVRTKRGAEVTYSGFAGNNAASVVSVGDAGKERQIVNVGAGAISDT
                     STDAINGSQLYAIAKTLKDDLDAINPTWTLKDDKGGSATINGGKDNKNGNTLTVKGDG
                     DIAVTVNDKGMTIDGTKLTENIKTDAIKIKDNNGGLLKKDLSDEIEITGKGPIKTQVA
                     GDKLEISAETASLPVNGGNGKITVNKGDDDKLVTAKNIAEAINQSGWKIKADKDTGGV
                     IEGNAQGTETLIKPGDMVTLKAGKNLSVKQSNGDFTFSVIDTPSFSSVQLNGNGKNGN
                     VTLSSEGNKLTLSGGNGAGQNGVVISGVASGLNNKDLAGLKADDAEMKNAASVADLKT
                     AIDG"
     gene            complement(2727..3188)
                     /locus_tag="ACHKJF_005060"
                     /pseudo
     CDS             complement(2727..3188)
                     /locus_tag="ACHKJF_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3496..3609
                     /locus_tag="ACHKJF_005061"
     CDS             3496..3609
                     /locus_tag="ACHKJF_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0952265.1"
                     /translation="MHEPPVQPDRCALSGNYRLGSNPVRHDLTPLAAAIGN"
     gene            complement(3853..4557)
                     /locus_tag="ACHKJF_005062"
     CDS             complement(3853..4557)
                     /locus_tag="ACHKJF_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2931 family protein"
                     /protein_id="HFP0952266.1"
                     /translation="MRRLPLMVGVIVGVMLAVGCHDRRAGVKTLLGDGMDQWGQKLPY
                     DHWQFNFFYPKNLPALVTMVYLEDGDVRESIFRQLDAARPSQSSVDTWSQRVGSFSAS
                     FNIGKALPVRMTVCWDSVIDKKAYETEIWFSRDTWQQMLTAYPDTYKPGKTYYRDNMI
                     IGLAPGGAVRIWLEDNGEPVVLQHPARQFTLTGDDMLICKNVPNQIDFSYIKANGYDP
                     FIRDFIKEKPYPYGHW"
     gene            complement(4568..6259)
                     /locus_tag="ACHKJF_005063"
     CDS             complement(4568..6259)
                     /locus_tag="ACHKJF_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFP0952267.1"
                     /translation="MVEEDEKNVPGDTCYYRYEIIGDSTHRTSIHNSFKTIQCRRVPV
                     RGITLTVGVFFDGTGNNRANADDLRLAYAHCANLVDEERARACEKYEKRARAGLGNAS
                     WQGGHTNISRLFDLYQQSDELNDSETEAQVKAYISGIGTADGESDSILGKALGSSLIR
                     QFEGVVTKTDEALEKISAELTRFIKLNRDKVAIAKVQFDVFGFSRGAAAARHFANRVM
                     NQDAAIAAAIDKGTDLTGHHGKLAGEVRFLGLFDTVAAIGSLLNFYDVNGRSNPGVNL
                     ELRPSVAQKVFQISAMHECRYNFSLNSIAGMWPELALPGAHSDIGGGYNAGEDEISLL
                     TMPDFEVTRDSADEMQTEVYRKAEKMRQVLYSLPALKYLMPHGEVETKMISWPLVNQN
                     KARASIFEKKVGAAVFMTRRDVPNDWEKVSLRVMLDAAQDAGAVFTPILSSNKELALP
                     SELLPLCDKAIAQGRAVRRGAEPEGFTAEDMRTIGRYLHCSANWNIDSDLSLWVSPSS
                     GEVFLRNNYAPTDERSFVWPMAPGKGWVRTVWRMDDQQQWEERARVNADALDGLF"
BASE COUNT         1497 a         1814 c         1286 g         1738 t
ORIGIN      
        1 ccatcaattg ccgttttcag gtctgcaaca ctggcggcat ttttcatttc cgcatcgtct
       61 gctttcaggc ctgccaggtc cttattattc aatccacttg ccacaccact gataacaaca
      121 ccattttgtc cggcaccatt accgcctgac aatgtcagct tattaccttc ggatgagagc
      181 gttacatttc cgttttttcc attaccgttc agctgaacac tgctgaatga cggagtatca
      241 ataacagaga atgtaaaatc accgttactt tgtttcacgc tgagattttt accggctttc
      301 agcgtaacca tatcaccggg ttttatcagt gtttctgttc cctgagcatt tccctcgata
      361 acaccaccgg tatctttatc cgctttaatt ttccagccag actggtttat ggcttcagca
      421 atgtttttcg cagtaacaag cttatcgtca tcacctttgt ttactgtaat cttcccgtta
      481 ccaccattta caggcaacga agctgtctct gctgaaatct ccagtttatc tccagccacc
      541 tgggttttta ttgggccttt gccagtgatt tcgatttcat ccgaaaggtc ttttttcaga
      601 agaccaccat tgttatcttt tatttttata gcatctgttt taatgttttc agtaagttta
      661 gttccgtcaa tcgtcattcc tttatcatta acagtaactg caatatctcc atcaccttta
      721 acagttaaag tgttaccatt cttattatcc ttcccaccat taatagtcgc agaacctccc
      781 ttatcatcct ttagcgtcca cgtcggatta atggcatcaa ggtcatcttt cagcgtcttc
      841 gctatcgcat ataactgact accgttaatg gcatctgtac ttgtgtcaga aatcgcaccc
      901 gcccccacgt taacaatctg acgctcttta cccgcatcac caacagatac tactgatgct
      961 gcgttatttc cggcaaagcc actatatgtg acttccgcgc ctcttttagt tctgacctta
     1021 gcctctgtta ctttttgtgc ctgcttatcc ctggagtcag cgccgatgga cacgttcccc
     1081 tttgcgacag taacggttgc tccatgcccc agggcaaccg tttcattctg agcggcattt
     1141 gcctctgaac caacagcaag agcatcgttt ttatttgcag aggactgact accaacggcc
     1201 agagcattac cggcattagc cagcgcatta aaaccaaccg caatcgccat atccccttta
     1261 gcttccgccg tggctccaat tgcattcgag gccatcccct cagcagaact caccgaacca
     1321 agcgatactg acaattcgcc tttagcaaaa gttctgctgc ccagcgcaat actgccatcc
     1381 cccttcgctt ccgatgccag atagttattc tctatttttt cccgaccata agtcttacca
     1441 tgcaaaggag ttaaatcatc cccaccaata acaatagccc cccatccggt agctttggta
     1501 tcgccaccta ttgcaattga ctgttcttta gttgctttcg ttttcgctcc tatcgcaata
     1561 gactcgcctg tgtacaaacc ttccttcgtg ctaatgtcac ctccagcctc tgcactggta
     1621 ccaatcgcga tagagttacg tccttcagcc accggtgatt tatggtcgaa attaattccc
     1681 tttgattcat cttttgctgt aaatttcgtt gtacctattt caatgctctt ccagtcatcg
     1741 ccatagacat catggccaag gctggtttca tcaacttcaa cagttaactt atttgtatct
     1801 gcctgagcac tggcctgtga tatggatgcc ggattaagaa caagtgatat ggctatacag
     1861 atatgttttt ttctgaggtt catatatacc ctcttttctc ctttattttc ttccactcag
     1921 tcggaatagc ataaaaatca tgcatgaata ttatgtaagg tggactgcct tcatttcttc
     1981 agcatccatt ttttataaag ccagaagtga acaaaacggg aaccattaaa aagcagacaa
     2041 aaggcaataa agtgatttat aataatgcga acataaaaca tattaagttt aagtaaacta
     2101 cccgacatta cgcgttacag cagaatgcag gtacactacc tgccattgac tgacgcaccg
     2161 gcgaaggcaa taaaaatgag atacatttca cttcgcagca aaaaacgaaa ttctacaatt
     2221 tttctgattt tactgacaga aaagcctggg agttaaattt aaataactta aaagattatg
     2281 tggtataatc aggttcatac aggcctgatt attttacggg gatgttaact ctttcaaatc
     2341 aacagtctac ggagaagcaa tgtggttatg gtcaataaat gttcactgat ttctttcacg
     2401 gtaatacccg gggcacttct tgtcctctgg taacaaaggt aactgtacat tcgtctttgc
     2461 aactgacagt gcatcacggt tttgaagtac ctgctgctgt tgttttttta ctcagaaagc
     2521 gcagttactc tttatatttt cccagcctat ctcttatttt aatatacatc cactgtctgg
     2581 cgttttaagg ggctggctca ctggccaagt tgacattaat catcattcct gtctttgtca
     2641 gcggagactt ctgctgtcag ttttttcatc catacagagt gacattaatt atccgtaagc
     2701 gcaccgtgaa ggacgtgggg taaaaattag tttacagata gagtgacgtt ccagggcaac
     2761 aactctttca cgcggttggc tggccaggtg ttgatgacac tgatcacgtg gcgtagccac
     2821 gtttccggct cgattccgtt aagtttgcag ctaccgatca ggctgtacat cactgccgca
     2881 ctctcgcctc ctccatcaga gccgaagaac atgtagttac gccgccccag tgcaactacc
     2941 cggagggcgt tttcacacag attattgtcg atctccaccc agccattgcg gcagtattcg
     3001 ttcagggcgt cccattgctt cagcagatag gtgaacgctt tcgccgtatc cgagtgacgc
     3061 gacagcgtgc tcatctgccc ctggagccac tcatacaacg actgcattag cggtaccgtt
     3121 ctggcttttc tgaccgccag tcgctcttct gccggactgc cgcggatctc agactcgata
     3181 gcgtacagtg cctcgatagg gctgcgtttg ggaaacgtac aatattgtac ggttgcgcta
     3241 ttgcggtagg gtgtactcca acgctatacg gtatttggca gacctttagg gacatgagat
     3301 aacctaaatg caataagaga aaagcaataa cttaaaggcg atagctgatc acgatgaatc
     3361 aatcagttac ttatccgcat cgattaaaaa atgctcctaa cgtacaataa ttccgtttcc
     3421 caaaccgacc cctctcatag ctcacgctgt tggtatctca gttcggtgta ggtcgttcgc
     3481 tccaagctgg gctgtatgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     3541 aactatcgtc ttgggtccaa cccggtaaga cacgacttaa cgccactggc agcagccatt
     3601 ggtaactaaa caaggtggat tccgatatta gagtcttgaa gtgatcaccc aactacggct
     3661 acactgaaag gacagtattc ggtatctgcg ctccaccaag ccagttacca cagtcgatta
     3721 gaagaggtcg aagacttcta atcggacgga aaaagccgcc gcaaggcggc tttttccgtt
     3781 tacaagccga agattacgcg cagcttaaaa ggatctcaag aagatcattt taattttgct
     3841 gatcgttgct ccctaccagt gaccatacgg atagggcttt tccttgataa aatcgcggat
     3901 aaatggatcg taaccattag ccttgatgta actgaaatca atctggttgg gaacgttttt
     3961 gcatatcagc atatcatctc ccgtcagagt gaactgccgg gcaggatgtt ggagtacaac
     4021 aggctcgcca ttatcctcca gccagatccg cacggcccct cccggtgcca atccgataat
     4081 catattgtcc cgataatacg tttttcccgg cttataggta tcaggatagg cagtcaacat
     4141 ctgctgccac gtatcccggc tgaaccagat ctccgtctca tatgcctttt tatcgataac
     4201 cgaatcccaa cacaccgtca tcctgaccgg cagtgccttg ccaatgttaa aactggctga
     4261 aaaactgcct acgcgctggc tccatgtatc cacactactc tgacttggtc tggctgcatc
     4321 aagctgccga aatatgcttt cccgtacatc accgtcctcc agatacacca ttgtcaccag
     4381 tgctggcagg tttttcgggt aaaaaaaatt aaactgccag tggtcataag gcagcttctg
     4441 cccccattga tccataccgt cgcccaacag cgtcttcacg ccagccctac ggtcatggca
     4501 gccgacagcc agcattacgc ccactatcac gcccaccatc agcggtaatc gtctcattcc
     4561 tgcctcctca gaacagtccg tccagcgcat ccgcgtttac ccgcgcccgc tcttcccact
     4621 gctgctggtc gtccatccgc cacaccgtcc gcacccagcc tttgcccggt gccatcggcc
     4681 acacgaacga gcgttcgtca gtcggagcat agttatttcg cagaaaaact tctcccgagg
     4741 acggactcac ccacaggctc aagtctgagt cgatgttcca gttcgccgag caatgcaggt
     4801 aacgcccgat ggtccgcata tcctccgccg tgaagccctc aggctccgcg ccacgccgga
     4861 cagcacgccc ctgagctatg gctttgtcac acaggggaag cagttctgac ggcaacgcca
     4921 gctctttgtt actgctgagt atcggagtaa acacagcccc ggcgtcttgt gccgcatcca
     4981 gcatcacccg caaactcacc ttctcccagt cgttcggcac atccctgcgc gtcataaaca
     5041 ctgccgcgcc cactttcttc tcaaaaatag acgcacgcgc cttgttctga ttgaccagtg
     5101 gccacgaaat cattttcgtc tccacctcac cgtgcggcat caggtacttc agcgccggca
     5161 gtgagtacag cacctgacgc atcttctccg ccttacggta cacctccgtc tgcatttcat
     5221 ccgcgctgtc gcgcgtcacc tcaaaatccg gcatcgtcag cagggagatt tcatcctctc
     5281 ctgcgttata gcccccgcca atgtccgagt gcgcccccgg cagcgccagc tccggccaca
     5341 tccccgcgat gctgttcagg ctgaagttat accgacactc atgcatcgcg ctaatctgaa
     5401 acaccttctg cgccactgaa ggacgcagtt cgagattcac cccgggattg ctgcgtccgt
     5461 taacgtcgta gaagttcaac aggctcccga tagccgccac tgtgtcaaac agccctaaaa
     5521 agcgcacctc tcccgccagt ttcccgtggt gcccggtcag gtctgtccct ttgtctattg
     5581 ccgccgctat cgccgcatcc tggttcatca cccggttggc aaaatgccgt gcagccgccg
     5641 cgccgcggct gaatccgaac acgtcaaact gcaccttcgc aatcgccact ttgtcacgat
     5701 taagtttgat aaagcgggtc agttctgcgc tgatcttctc cagtgcttca tcggtctttg
     5761 tcaccacccc ttcaaactgt cggataaggc tgctgcccag cgctttaccc agtatcgaat
     5821 cactttctcc gtctgccgtc ccgatgccgc tgatataggc tttgacctgc gcctcagtct
     5881 cgctgtcgtt aagttcatcg ctttgctgat aaagatcgaa cagccgggaa atattagtgt
     5941 ggccgccctg ccagcttgca ttacccagcc cagctctggc gcgcttttca tacttctcac
     6001 atgccctagc ccgctcctcg tcaaccagat tggcacagtg ggcatatgcc agccgtagat
     6061 cgtctgcgtt cgcccggtta ttccccgtcc cgtcaaagaa cacccccacc gtcagcgtta
     6121 tccccctgac cggcacccgc cgacactgga tggttttaaa actgttgtgt atgctggttc
     6181 gatgggtact gtccccaata atttcatacc ggtaataaca ggtatcgccc ggcacatttt
     6241 tttcatcttc ttcgaccata aaaatcctta tttatctgaa agataaaatc agtagcaaaa
     6301 tccttcggga cttttttaca tctgcctgtt agacg
//
LOCUS       DAZGXZ010000225         6312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000225 DAZGXZ010000000
VERSION     DAZGXZ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.226"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            241..750
                     /locus_tag="ACHKJF_005064"
     CDS             241..750
                     /locus_tag="ACHKJF_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0952268.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPVSNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEEDYEEKLLIARWELTSEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHIDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            722..2650
                     /locus_tag="ACHKJF_005065"
     CDS             722..2650
                     /locus_tag="ACHKJF_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0952269.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRIVPMTAVEWADQNYYLPKES
                     SYGDGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVETTIRDVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSEPDQSNGRWICENTGMWTRDGLMFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVDEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSRDEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            2634..2840
                     /locus_tag="ACHKJF_005066"
     CDS             2634..2840
                     /locus_tag="ACHKJF_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP0952270.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            2837..4429
                     /locus_tag="ACHKJF_005067"
     CDS             2837..4429
                     /locus_tag="ACHKJF_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0952271.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYHGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVRAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILEKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4419..5924
                     /locus_tag="ACHKJF_005068"
     CDS             4419..5924
                     /locus_tag="ACHKJF_005068"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0952272.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGLRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYAGAHKVDGN
                     QFEALPAEVRQDMQQRIDAAHRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMATALNSNARGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILSAAAPQQPVASSQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKASPILAASPQADTGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVENARAVLA
                     AGSGKAEPISASTTALFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            5961..6308
                     /locus_tag="ACHKJF_005069"
     CDS             5961..6308
                     /locus_tag="ACHKJF_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0952273.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EASGKLVVWDGQKAGGAVGILVLPLEGTETVLTYYKSGTFATEAIHWPESVDEHKKAN
                     AFAGSALSHAALP"
BASE COUNT         1453 a         1531 c         1948 g         1380 t
ORIGIN      
        1 cattatcatt ttctcgggtc ctttccggca tatggacccg ttacggggcg gcgacctcgc
       61 gggttttcac tatttatgaa aattttccgg gatccatgtc cggtttccct gcaagttaac
      121 tatatgaaaa atataaaaac aggctttccg tgaaccggac atgcgcaaaa aatgaacatg
      181 taaaccggac atgaccggtt ttgtgttgat tgtgaggtga gagtttttgc gaggtgagga
      241 gtggctaccc agactgaagt tgccaggcat ttaagtctga ccgaccgcca gcttcgcaga
      301 ttgcagaaat taccgggtgc cccggtctcg aataagcgag gacaactgga tctggatgcc
      361 tggcgtgatt tttacatatc gtatctgagg agaagtaaaa acgatgtgcc tgatggcgat
      421 agcgaagaag actatgaaga aaagttactt attgccagat gggaactgac atcagaacag
      481 gccgttacac aacagctaaa aaatgaggtg tcaaaaggaa aactgattga caccggattc
      541 tgtatttttg ccctcagcaa gctggcgatg gcattgtcca gtacgcttga ttctattccg
      601 ttatccatgc agcgacagtt cccggattta acgccacgtc atattgacca tctgaaaacc
      661 cttattgcga agggcgcaaa tcagtgtgcg cgggcagggg ataaattacc ggatttgctg
      721 gatgaatata tcagagcaac aactgaataa tatgatggct gccgtttcgg ttgcgctgca
      781 gcccctggtc aggattgtgc cgatgacggc agttgaatgg gctgatcaaa attattatct
      841 gcctaaagaa tcctcatacg gtgacggcga atggaaaacg ctgccgttcc agatcgccat
      901 catgaacagt atggggaatg atcagatccg cactgttaat ctgattaaat ctgcccgtgt
      961 tggctataca aagatgttgc tgggggtggt cgggtatttt attgagcata aatcccgaaa
     1021 cagtctgctt tttcagccca cggattctgc cgctgaagat tttatgaagt ctcacgtgga
     1081 gacgacgatt agggacgtgc catgcctgaa agacctttcc ccatggctgg gtcgtaaaca
     1141 tcgtgataat accctcacgc tgaaacgctt ttcatcgggc gtcggtttct ggtgcctggg
     1201 cggcgctgcc gccaaaaact accgtgaaaa atccgtggac gtggtctgct atgacgaact
     1261 ttcctcgttc gaaccggatg ttgaaaaaga gggttcgcca accctgcttg gggataaacg
     1321 tattgagggc tctgtatggc caaaatccat tcgcggctcg acgcctaaaa tcaaaggcac
     1381 ctgccagatc gaaaaagcgg ccaacgagtc ggcgcatttc atgcgttttt atgtgccctg
     1441 cccacactgt ggggaggagc agtatctgaa atttggcgat gaatccacgc cttttggcct
     1501 taaatgggag aaggacagcc ccgaaagcgt tttctacctc tgtgaacatc atggctgcgt
     1561 gatccatcag tctgagcctg accagagcaa cgggcggtgg atctgtgaaa acacgggcat
     1621 gtggacccgt gacggcctga tgtttttcag cgcccggggt gatgaaattc cgccgccgcg
     1681 ctccatcact ttccatatct ggacggcgta cagtccgttc accacctggg tacagatagt
     1741 ctatgactgg ctggatgcac tgaaagatcc caacggcctg aaaacctttg tgaacaccac
     1801 gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg tactgatgga
     1861 taaggttgtg cgttacacgg cggcggtgcc tgcccgggtg gtttatctga cggcgggcat
     1921 tgactcgcag cgaaaccgtt ttgagatgta tgtctgggga tgggctccgg gagaggaagc
     1981 ctttctggtg gataaaatca tcattatggg gcgtcctgat gaggaagaga cgctgttacg
     2041 tgtggacgcg gcgatcaaca aaaaataccg ccatgcggat ggcaccgaaa tgaccatttc
     2101 ccgtgtctgc tgggacaccg gggggatcga tggcgaaatc gtttatcaga ggtcaaaaaa
     2161 acacggtgtt ttccgggtgc tgccggtaaa aggtgcatct gtttatggca agccggtgat
     2221 caccatgcca aaaacccgca atcagcgggg cgtgtatctg tgcgaagtgg ggacggacac
     2281 cgcaaaagaa attctctatg cccgtatgaa agccgatccc acgcctgtgg atgaggccac
     2341 gtcgtatgcc atccgttttc ctgatgatcc ggagattttt tcgcagacag aggcgcagca
     2401 actggtcgcg gaagagcttg tggagaagtg ggaaaaagga aagatgcgtc tgctgtggga
     2461 taacaaaaag cggcgtaacg aagcgctgga ctgtctggtg tatgcctacg cggcattacg
     2521 tgtgtccgtg caacgctggc agcttgatct ggctgtactg gcaaaatccc gggacgaaga
     2581 gacgacccgg ccaaccctga aagaactggc agcgaagctg tccggaggag tgaatggtta
     2641 cagtcgctga actgcaggcg ctgcgtcagg cgcgccttga tttattaacc ggtaaacggg
     2701 tggtgtctgt ccagaaagat ggtcgcagaa ttgaatatac ggcggcttct ctggatgagc
     2761 ttaaccgtgc gatcaatgat gcggagtcgg tactggggac aacccgacgt cgccgtcgtc
     2821 cgctgggagt gaggttatga aacgaacgcc tgtcctgatt gatgtgaacg gcgttccgct
     2881 tcgggagagc ctcagctacc acgggggcgg tgcaggattt ggcgggcaaa tggcggagtg
     2941 gttgccaccg gcgcagagtg ccgatgcggc cctgctgccc gcgttgcgtc tggggaatgc
     3001 ccgtgcagat gatctggtac gcaataacgg gatagcggct aatgcggtgg ccctgcataa
     3061 ggatcatatt gtcgggcata tgtttctgat cagctaccgt ccgaactggc gctggctggg
     3121 gatgcgggag actgcggcaa aaagttttgt cgatgaggtg gaggcggcct ggtcggaata
     3181 cgcagaaggg atgtctggcg agatcgacgt ggaagggaaa cgcacgttta cggaatttat
     3241 ccgtgaaggt gtgggcgttc atgcgtttaa cggcgaaatc tttgtgcagc cggtctggga
     3301 tacggaaacc acgcagttat tccgtacgcg ttttaaagcc gtgagtccga aacgggtgga
     3361 cacgcctgga cacggtatgg ggaaccgttt tctgcgggcc ggtgtggagg tcgatcgata
     3421 tggccgtgcc gtcgcgtacc atatctgtga ggatgatttt ccgttctctg gtagtggacg
     3481 atgggaacgg atcccgcgtg aacttcccac cgggcgcccg gccatgctgc atattttcga
     3541 gccggtggag gacgggcaga cccgtggggc caatcagttt tacagcgtca tggaacggct
     3601 gaagatgctc gattccctgc aggcaacaca gcttcagtcg gccatagtga gggcgatgta
     3661 tgcagcgacg attgaaagtg aacttgatac cgaaaaggcc tttgaatata tcgccggtgc
     3721 gccgcagggg cagaaggata atccgcttat taatattctg gagaagttct ccacctggta
     3781 tgacacgaat agcgtgacgc tgggtggtgt caaaattccg caccttttcc ccggggatga
     3841 tctgaaactg cagaccgcgc aggattcaga caatggattt tcggcgcttg aacaggcgct
     3901 gctgcggtat atcgccgccg gtcttggcgt ttcctacgaa cagttgtccc gtgattactc
     3961 gaaggtcagt tattcaagtg cccgcgcctc cgccaatgag tcgtggcgct attttatggg
     4021 acgacgaaaa tttattgcgt cccggctggc cacgcagatg ttttcctgct ggctggaaga
     4081 ggcacttctt cgggggatta ttcgtccgcc acgggcgcgt tttgattttt atcaggcgcg
     4141 ttcagcctgg tcacgggccg agtggattgg tgccggaaga atggccattg acgggctcaa
     4201 ggaagtccag gaatcggtga tgcgtattga ttccggactg agcacgtatg agaaagagct
     4261 ggcgctgatg ggtgaggatt atcaggacat tttccgccag caggtcaggg aatccgcaga
     4321 gcgggaaaag gccggactct cacgtccggt gtggatagca caggcgtatc agcagcagat
     4381 agcggagagc cgcaggccgg aagaggagac aacaccacgt gagacgtaat ctttcacaca
     4441 ttattgccgc agcattcaat gaaccgctgc ttctggagcc cgcctatgcg cgggttttct
     4501 tttgcgcgct cgggcgcgag atgggggcag caagtctttc ggtaccacaa cagcaggtac
     4561 agcttgatgc tcccggaatg ctggctgaaa cggacgagta catggccgga ggaaaacgac
     4621 cggcccgtgt ttaccgggtg gtgaacggca ttgctgtact gccggtgaca ggcacgctgg
     4681 tgcaccggct gggggggctg cggccctttt ccggaatgac aggctatgac ggcattgtcg
     4741 cctgtcttca gcaggcaatg gcggatagcc aggtgcgtgg cgtactgctg gacattgaca
     4801 gtccgggcgg gcaggccgcc ggcgcgtttg actgcgctga catgatttac cgcctccgtc
     4861 agcagaagcc ggtctgggca ctgtgcaatg acacggcctg ttctgcagcc atgctgctgg
     4921 cgtcggcctg ctcccgacgg ctggttaccc agacatcccg tatcggctcc attggcgtga
     4981 tgatgagcca tgtcagctat gccggtcatc tggcgcaggc cggtgtggat atcacgctga
     5041 tttacgccgg ggcgcataag gtggatggca atcagtttga agcgttgccg gcagaagttc
     5101 gccaggacat gcagcagcgg attgatgcgg cacaccggat gtttgccgaa aaagtggcga
     5161 tgtataccgg tctgtctgtg gatgccgtca cgggaacaga ggccgccgtt tttgaaggtc
     5221 agtccggtat tgaggccggg ctggcggatg aattaatcaa tgcgtcggat gccatcagtg
     5281 tgatggccac ggcgctgaac agtaatgcca gaggaggcac tatgccgcaa ttaactgcaa
     5341 cggaagccgc cgcgcaggag aaccagagag tgatggggat cctgacatgt caggaagcga
     5401 aaggacgtga acagcttgcc acgatgctgg caggacaaca gggcatgagc gttgaacagg
     5461 cccgggcgat tctgtccgcg gcagcaccac agcaaccggt ggcatcctcg cagagtgaag
     5521 ccgatcgcat tatggcgtgt gaagaggcta aaggtcgtga acaactggcg gcaacgctgg
     5581 cggcgatgcc ggagatgacg gtggaaaaag ccagccctat cctggctgct tcaccacagg
     5641 cagataccgg cccctcactc cgtgatcaga tcatggccct ggatgaggca aaaggggctg
     5701 aggcgcaggc tgaacagctg gctgcctgcc cgggcatgac cgtggagaat gcccgggctg
     5761 tgctggctgc gggatcaggt aaggcagagc ctatctctgc atccacaacc gccctgtttg
     5821 aacgcatcat ggcgaaccat tcaccggcag cggtacaggg gggcgtgtca cagacgtcag
     5881 cagacggtga tgcggacgtg aaaatgctca tggccatgcc atgaagtcag tgctgaccat
     5941 caatatgagg tttttacaat atggtaacga aaaccatcac tgaacagcgt gcggaagtac
     6001 gtatttttgc cggtaatgat ccggctcata ccgccactgg cagcagcggg atttcttctg
     6061 caacaccggc tctgacgccc ctgatgctgg atgaggccag cgggaaactg gtggtctggg
     6121 acggacagaa agccggtggt gcggttggca tactggtact gccgcttgaa ggtacagaga
     6181 cggtactgac gtattacaag tcggggacct ttgcgacaga ggcaattcac tggcctgaga
     6241 gtgtggatga acacaaaaag gccaacgcct ttgccggcag tgcccttagt cacgcggcgc
     6301 tgccgtaaca cg
//
LOCUS       DAZGXZ010000226         6051 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000226 DAZGXZ010000000
VERSION     DAZGXZ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6051
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.227"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..213
                     /locus_tag="ACHKJF_005070"
     CDS             <1..213
                     /locus_tag="ACHKJF_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0952274.1"
                     /translation="AWLFGHTARPLTRTEMVVVFGVVFHEYAFTFLADQLGISMKTVC
                     AHLYNAMEKNGLRGVSIKYLCSTADR"
     gene            309..842
                     /locus_tag="ACHKJF_005071"
     CDS             309..842
                     /locus_tag="ACHKJF_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0952275.1"
                     /translation="MKEYINPGKFIISGMQTDNVVIISSSRISEIVGFLYKNNISDMN
                     TLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMSVEKLTLSGRQVNV
                     LMMLLYGSNGTEGARHLGMSEKTFSTHKQNLIKRLRVHNEVELLYKGMLLVRKREVVK
                     QDSLFPVMLNAASDSLI"
     gene            861..1049
                     /locus_tag="ACHKJF_005072"
     CDS             861..1049
                     /locus_tag="ACHKJF_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0952276.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            1193..2041
                     /gene="mscS"
                     /locus_tag="ACHKJF_005073"
     CDS             1193..2041
                     /gene="mscS"
                     /locus_tag="ACHKJF_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0952277.1"
                     /translation="MVRNQDMLPGYAANLVAAVVIIIIGGIAARMISGVLNNLMLGRN
                     IDATVALFLSALIRHAIIAFTLIAALGRVGVQTASVIAVLGAAGLAVGLALQGSLSNL
                     AAGILLVMFRPFRAGEYADLGGVAGTVQNVHIFSTTMRTLDGKIVVIPNGKIIAGEIV
                     NFSREPERRNEFIISVSYDADIDCVKQVLTDIVMSEERVLKNREITVRLNEPGESSMN
                     FVVRVWSRRDDLQSVYWDVLERIKREFDSEGISFPYPQMDIHLVRSGKQAESVTHNSR
                     SVSSRQ"
     gene            2167..3387
                     /locus_tag="ACHKJF_005074"
     CDS             2167..3387
                     /locus_tag="ACHKJF_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HFP0952278.1"
                     /translation="MPKKIMTGIDVFDAAMERIEWVFDTFSSVCLSFSGGKDSTVLFH
                     LMANIARRKKRRFSVLFVDWEAQYQCTIEHVTRLREMYDDITDTFFWVALPLTTVNGV
                     SQVQPEWVCWEDGAEWVRQPPEDAITRASFFPFYQNAMTFEEFIPAFNHWFAREKGMV
                     AAILTGVRADESLNRFMGLVSGRKLRYAEDKPWTTASAEGFYYTMYPLYDWKTRDVWI
                     YHARTRAAYNSLYDLMYRAGVPLRNMRVCEPFGPEQRKGLWLYHVLEPDTWGRMCARV
                     SGAVGGALYANESGAYFALRKQITKPVHHTWQSYAMFLLESMPEKTAEHYKNKIAVYL
                     RWWQSHGWPDGIPDEQDNDQGCKDIPSWRRICKTLLKNDFWCRMLAFSPNKPRHYERY
                     LARMREKRKEWGIL"
     gene            3617..5362
                     /locus_tag="ACHKJF_005075"
     CDS             3617..5362
                     /locus_tag="ACHKJF_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0952279.1"
                     /translation="MTAGEKRVASRLEAFMSNECLVWYDIPVGRKNRHPDFVIIDPEN
                     GLVFLEVKDWTVHTLHQVNHEQVILETNGILKSEINPLLQVRRYACDTVNALPADSRL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKALAGNDENAVERIFPSAQTICQDDMTQS
                     VLPDIFRQKIAGMFTMGFRTRVTPQVRDILRAHLFPEMTVRQHSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIESRGL
                     KSRVTVFHFHSWCASMIKRHGIQVTAGGKDYPEKCFSALEDAVNSGTITDTGYDAVLV
                     DEGHDFESRWLALIARLFDNASRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNTKRILHFAYAFSREYFEKHQNREIPFVQPQAGGEEGTEPEILRCASESDE
                     AVQVVGWLEKRYTLCGHWSDMAVLCPAEFSVKHLKEVMTQRGIPYATCFDSEGKKKYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVHSGAADESEVIPALYVAFTRATRELLVT
                     CYRENSISRHLEDFV"
     gene            complement(5544..>6051)
                     /locus_tag="ACHKJF_005076"
     CDS             complement(5544..>6051)
                     /locus_tag="ACHKJF_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP0952280.1"
                     /translation="AEPVDCVIWIKNNLIEANDYNPNVMAPSEKRLLTHSLLVDGFTQ
                     PVVTLAQKGKYTVIDGFHRQMLGKSTTKLEKKLKGYLPVTCIKHDNNFKAYSIASTIR
                     HNRARGKHQIDALSDIVRDLSRMGWDDLRIGSELGMDTDEVLRLKQISGLTELFEEEN
                     FSPAWTIK"
BASE COUNT         1519 a         1162 c         1587 g         1783 t
ORIGIN      
        1 gcgtggctgt tcggacatac ggcaagacca ctgacccgta ccgagatggt ggtggtcttc
       61 ggagttgttt tccatgagta cgcttttacc tttctggcag accagctggg gataagcatg
      121 aagacggtat gtgcgcatct ttacaatgcg atggagaaaa atggtctgcg cggcgtcagt
      181 attaaatatc tctgcagcac cgcagaccgg taaatccggg ataattaatg ttatgtggcc
      241 aggcgtgata aatttcatgt ggaacgggta tgcattttat ttgtgataac agttattttc
      301 tggagggagt gaaggagtat attaatcccg gtaaattcat tatcagcggc atgcagacag
      361 ataatgtggt gataatatct tcttcccgga tatctgaaat tgtcggtttt ctgtataaga
      421 ataatataag tgatatgaac accctttatt tttcttctcc ggaggcgctc cgttttctct
      481 ggggagtggt tgacagtccg gtttatgata tcagacatct tactgagcga tgttcagtaa
      541 acgatatcag tcatctgtac cgcgcattca tgtcggtgga gaagctgacc ctgtcaggcc
      601 ggcaggtaaa tgtactgatg atgctgcttt atggcagtaa cggaacagag ggggcacgac
      661 atcttggcat gtcagagaaa acattctcca ctcataagca gaatctgata aagaggttac
      721 gggtacataa tgaagttgag ttgttgtaca aggggatgct tctggtcaga aaaagggagg
      781 ttgtgaagca ggacagctta tttccggtaa tgttaaacgc agcctctgat tcgttaattt
      841 aataaacata aggagttttt atgcgtctgt tgattgcttt tcttcttccc tggctgactt
      901 ttttcaccat tggccggcct ctgacgggga ttttttgtct ggtgttacag ataacgctgc
      961 tgggctggat accggcaaca atatgggccg tgtatgcgct ttcgcagtat aagacggaac
     1021 agaaaatccg tgaagccacg gggaaatgat gactgcagaa gagcgggttg tcatagctgt
     1081 gtatatgatg cgggataaag gcccggagat tcctggaaat aaaatgttcc ggggaatata
     1141 aaacagggaa aaaccggatg tgatggacgc ttctggcgag gcggtttcat gaattgtccg
     1201 taatcaggat atgttgcccg gttacgccgc gaatcttgtg gcagcagtgg ttattattat
     1261 tatcgggggt atcgctgcac ggatgatttc tggtgtgctg aataatctga tgctgggcag
     1321 gaatattgat gccacagtgg cgctgttcct ttccgcgctg atacgtcatg ccattatagc
     1381 ctttacgctg attgctgccc tgggacgtgt gggggttcag acagcttccg taattgcagt
     1441 cctgggggct gcaggtcttg ctgttggact tgccctgcag gggtcattgt caaaccttgc
     1501 agctggtatt cttctcgtga tgttccgtcc tttcagggct ggtgaatatg ctgacctggg
     1561 tggtgttgcc gggactgtgc agaatgtaca tatattctca accacgatgc gtacactgga
     1621 tggtaaaatt gttgtcattc cgaacgggaa gattattgcc ggagagattg ttaatttctc
     1681 cagggagcct gaacgacgaa atgagtttat catcagtgtt tcatatgatg cagatattga
     1741 ctgtgtgaaa caagtcctga cagatattgt gatgtcagaa gagagggttc tgaagaacag
     1801 ggaaatcact gttcgcctga atgagcctgg ggagtcatcc atgaattttg tagtacgggt
     1861 atggagcagg cgtgatgacc ttcagtctgt atactgggat gtactggaga gaattaagcg
     1921 ggagtttgat tcagaaggga taagttttcc atatccacaa atggatatcc atcttgttcg
     1981 ctccgggaag caggcagagt cggtaaccca taacagtcgc tcagtcagtt ccagacagta
     2041 aaatgaaaag cgcattttat ttgtgaaaga tgaacagcct gaatctgtca ggctgttttc
     2101 attgtgtttt tatggtcatt tttggtatat gtactgtata taatgtcatc tgtacaagga
     2161 tataaaatgc caaagaaaat aatgacaggg attgatgttt ttgacgctgc catggagcgt
     2221 attgaatggg tgtttgatac gttttcatca gtttgtcttt ctttttccgg cggaaaagac
     2281 tccactgtcc tgtttcacct gatggcgaat attgcccgcc gaaaaaaacg ccgtttttct
     2341 gtactgtttg ttgactggga ggctcagtat cagtgtacca ttgagcatgt caccaggttg
     2401 agggaaatgt acgatgatat cacagatact tttttctggg tggcgctgcc tctgacgacc
     2461 gttaatggag tgtctcaggt acagcctgag tgggtatgtt gggaggacgg ggctgaatgg
     2521 gttcgccagc caccagaaga tgccataacc agagcgtctt tttttccttt ttatcagaat
     2581 gcgatgacat ttgaagagtt tattccggca tttaatcatt ggtttgcccg ggaaaaaggg
     2641 atggttgctg cgattcttac tggtgttcgc gctgatgagt cactgaatcg ttttatggga
     2701 ctggtatccg gacgtaagct ccgttatgcg gaggataaac cctggaccac ggcatcggct
     2761 gagggatttt actacaccat gtatcctttg tacgactgga aaacaagaga tgtatggatt
     2821 taccatgcga gaacacgtgc tgcctataac tctctttatg acctgatgta cagggctgga
     2881 gttcctcttc gcaatatgcg ggtgtgtgag ccttttggac cggagcaacg taaggggttg
     2941 tggctttatc atgtactgga gccggataca tggggcagaa tgtgtgctcg cgtatcaggt
     3001 gctgtagggg gagcgctcta tgcaaatgaa agtggtgctt attttgcttt gaggaaacag
     3061 ataacaaaac cagttcacca tacatggcag agttatgcaa tgtttctgct ggagtcaatg
     3121 cctgagaaaa cggcggagca ttacaagaat aagattgctg tttatcttcg gtggtggcag
     3181 tctcatggat ggccggatgg catccctgat gagcaggata atgaccaggg atgtaaagat
     3241 attccctcct ggcgtcgcat ctgtaagacg ttactgaaaa atgatttctg gtgtcggatg
     3301 ctggcgttta gtccgaataa gccccgacat tatgagcgct acctggccag aatgcgtgaa
     3361 aaaaggaaag agtggggaat attgtaaatt atatttaaac atcagaaata tgacggttaa
     3421 tccaggatag tagctaaaaa aataagattc cttgctgatt tatccctgtt tgatatgtaa
     3481 aatctgaggg gcttataagc aacggatata cgagtgctac aatatatgat aagttatata
     3541 tattaaatgt tcatcatctg tcaggggctg ttttatggct aaactgataa atatatcagg
     3601 tgttaccgag gagcggatga ctgccgggga aaagcgggtt gcatcccgtc ttgaggcatt
     3661 tatgagtaat gagtgtctgg tctggtacga tattccggtg ggacggaaaa accgacatcc
     3721 tgattttgtc attattgacc cggagaatgg tctggtattt cttgaagtca aggactggac
     3781 cgttcacacg ttgcatcagg taaatcacga acaggtcatt cttgaaacta acggtattct
     3841 taaatcagaa ataaatccgc tgctacaggt tcgtcgttat gcctgtgata ccgtaaatgc
     3901 tttacctgca gactcacgtt tgcgacagaa tgatggtcag tataaggggc gtctgaatct
     3961 ggcctgggcg tatggtgttg tgtttacccg tattacccgc cagcaactga aggctctggc
     4021 tgggaatgat gaaaatgctg tggaaaggat tttcccttca gcgcagacaa tctgtcagga
     4081 tgatatgact cagtctgttt tacctgatat attccgccaa aaaattgccg ggatgtttac
     4141 gatgggattc agaacccggg tgacaccaca ggttagagat attctgcgtg ctcatctgtt
     4201 tcctgaaatg accgtcagac agcactcaca aatcaaaata atggatattc agcaggagat
     4261 tctggccaga aatattggtg atgggcatcg tgttattcat ggggtagccg gctctggaaa
     4321 aacaatgatt cttttgttcc gttgtcttta cttggctgaa actacccctg gtaagattct
     4381 ggtgctatgt tacaacatta ctctggccag ctacctgcgg gaatgtattg agtccagggg
     4441 actgaagtca cgggttacgg tattccattt tcatagttgg tgcgcctcaa tgataaagcg
     4501 ccatggaatt caggtgactg ctggaggaaa ggattacccg gagaaatgct tcagtgcgct
     4561 ggaagatgcc gtgaacagcg gaacaattac tgatactgga tacgatgccg tacttgtgga
     4621 tgaggggcat gattttgaga gtcgctggct ggctctgata gcccgcctgt ttgataacgc
     4681 gtcccgttca ctgctcctga tgtacgatga cgcacagtcc atttacagac gggaacgggc
     4741 tctgaacttt tcgcttgcca gtgtggggat tcaggcgcag ggacgtactt ccgtcctgcc
     4801 ggtaaattac cgtaatacga aacgcattct gcactttgct tatgcattct cccgtgagta
     4861 ttttgagaag catcagaacc gtgaaatacc gtttgttcag ccacaggcgg gcggtgaaga
     4921 gggcacagag ccggaaattc tgcgttgtgc ttctgagagt gatgaggcag tgcaggttgt
     4981 gggctggctg gagaaacgct atacactgtg cggtcactgg agtgatatgg ctgttttgtg
     5041 tcctgcagag ttttcagtga aacatctgaa agaggtcatg acacaacgcg ggatacctta
     5101 tgccacctgc tttgacagtg aaggtaagaa gaaatattcg cgacgcaagg atgttgtgca
     5161 tttactgacc taccagagca gtaagggact ggagtttccg tatgttgcgg ttatcaatgc
     5221 ttctttcgtt cactcaggtg ctgcggatga gtctgaagtt attccggcgc tttatgttgc
     5281 gttcacccgg gctacacggg aattactggt gacctgttat cgtgaaaaca gtatcagtcg
     5341 tcatcttgag gattttgtat aagttgtgtt ttattattgc tgatatgtaa tcattcgttt
     5401 tttgataatc taagatgggt atttagcgag tattcagagt tgaattaata tcccccatgt
     5461 ttttgatctg tcaatagctg tccattacat ggggtgatca tatatcagtc ggtaattact
     5521 ggataaagtc accccctaaa tgattacttt attgtccatg ccggactaaa gttttcctcc
     5581 tcaaatagtt ctgttaaccc acttatttgt tttaatctca gtacctcatc tgtatccatg
     5641 cccagttcac tacctattcg gagatcatcc catcccattc gggaaagatc tcggacgata
     5701 tcggagagcg cgtctatctg gtgctttcct ctggccctgt tatgcctgat ggtcgaggcg
     5761 atagagtaag ctttaaagtt attatcatgc ttaatgcatg tgacaggtaa atatcctttt
     5821 agttttttct ctagttttgt tgtgcttttc ccaagcattt gtctgtgaaa tccgtcaata
     5881 acggtgtatt tccccttttg agctaaggtg acaacaggtt gagtgaatcc atcaaccaac
     5941 agggagtgtg ttaataacct tttttcggag ggagccatga cgttaggatt atagtcgttt
     6001 gcttcaatta gattgttttt tatccatata acacaatcaa caggttctgc g
//
LOCUS       DAZGXZ010000227         6035 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000227 DAZGXZ010000000
VERSION     DAZGXZ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.228"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            219..866
                     /gene="fucA"
                     /locus_tag="ACHKJF_005077"
     CDS             219..866
                     /gene="fucA"
                     /locus_tag="ACHKJF_005077"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP0952281.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            894..2042
                     /gene="fucO"
                     /locus_tag="ACHKJF_005078"
     CDS             894..2042
                     /gene="fucO"
                     /locus_tag="ACHKJF_005078"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP0952282.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACHKJF_005079"
     CDS             complement(2097..2852)
                     /gene="xni"
                     /locus_tag="ACHKJF_005079"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP0952283.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLKQLRLVR"
     gene            complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACHKJF_005080"
     CDS             complement(2964..4331)
                     /gene="sdaB"
                     /locus_tag="ACHKJF_005080"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP0952284.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACHKJF_005081"
     CDS             complement(4389..5678)
                     /gene="sdaC"
                     /locus_tag="ACHKJF_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP0952285.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         1516 a         1487 c         1612 g         1420 t
ORIGIN      
        1 ttttgataaa tatttttaag agcggtcatt tttaatgggg ttataaaaag agaacttgca
       61 gttatttact gtgattagtt gaaccaggtc acaaaatata agcttgtgaa tttctttctg
      121 gttaatcagg aagaagatgg tgggtaatta aacggctaat tcaatagtgt gaaaggaaca
      181 acattattgc cctgttttga atcagagaga ggtagctaat ggaacgaaat aaacttgctc
      241 gtcagattat tgacacttgc ctggaaatga cccgcctggg actgaaccag gggacagcgg
      301 ggaacgtcag tgtacgttat caggatggga tgctgattac gcctacaggc attccatatg
      361 aaaaactgac ggagtcgcat attgtcttta ttgatggcaa cggtaaacat gaggaaggaa
      421 agctcccctc aagcgaatgg cgtttccata tggcagccta tcaaagcaga ccggatgcca
      481 acgcggttgt tcacaatcat gccgttcatt gcacggcagt ttccattctt aaccgaccga
      541 tccccgctat tcactacatg attgcggcgg caggtggtaa ttctattcct tgcgcgcctt
      601 atgcgacctt tggaacacgc gaactttctg aacatgttgc gctggctctc aaaaatcgta
      661 aggcaacttt gttacaacat catgggctta tcgcttgtga ggtgaatctg gaaaaagcgt
      721 tatggctggc gcatgaagtt gaagtgctgg cgcaacttta cctgacgacc ctggcgatta
      781 cggacccggt gccagtgctg agcgatgaag agattgccgt agtgctggag aaattcaaaa
      841 cctatgggtt acggattgaa gagtaatttc gtaaagcaac aaggagaagg atgatggcta
      901 acagaatgat tctgaacgaa acggcatggt ttggtcgggg tgctgttggg gctttaaccg
      961 atgaggtgaa acgccgtggt tatcagaagg cgctgatcgt caccgataaa acgctggtgc
     1021 aatgcggcgt ggtggcgaaa gtgaccgata agatggatgc tgcagggctg gcatgggcga
     1081 tttacgacgg cgtagtgccc aacccaacaa ttactgtcgt caaagaaggg ctcggtgtat
     1141 tccagaatag cggcgcggat tacctgatcg ctattggtgg tggttctcca caggatactt
     1201 gtaaagcgat tggcattatc agcaacaacc cggagtttgc cgatgtgcgt agcctggaag
     1261 gactttcccc gaccaataaa cctagtgtac cgattctggc aatccccacc acagcaggca
     1321 ctgcggcaga agtgaccatt aactacgtga tcactgacga agaaaaacgg cgcaagtttg
     1381 tttgcgttga tccgcatgat atcccgcagg tggcgtttat tgacgctgac atgatggatg
     1441 gtatgcctcc agcgctgaaa gctgcgacgg gtgtcgatgc gctcactcat gctattgagg
     1501 ggtatattac ccgtggcgcg tgggcgctaa ccgatgcact gcacattaaa gcgattgaaa
     1561 tcattgctgg ggcgctgcga ggatcggttg ctggtgataa ggatgccgga gaagaaatgg
     1621 cgctcgggca gtatgttgcg ggtatgggct tctcgaatgt tgggttaggg ttggtgcatg
     1681 gtatggcgca tccactgggc gcgttttaca acactccaca cggtgttgcg aacgccatcc
     1741 tgttaccgca tgtcatgcgc tataacgctg actttaccgg tgagaagtac cgcgatatcg
     1801 cgcgcgttat gggcgtgaaa gtggaaggta tgagcctgga agaggcgcgt aatgccgctg
     1861 ttgaagcggt gtttgctctc aaccgtgatg tcggtattcc gccacatttg cgtgatgttg
     1921 gtgtacgcaa ggaagacatt ccggcactgg ctcaggcggc actggatgat gtttgtaccg
     1981 gtggcaaccc gcgtgaagca acgcttgagg atattgtaga gctttaccat accgcctggt
     2041 aaatgcgctg atgtgataat gccggatacg acgtttgcgg cgtatccggc tcgccgttac
     2101 cgtaccaacc gcaattgctt taaattgccg tcgatatgca aatcggtttg taagcgggca
     2161 atatcgcggc acagaaacgc catctcttta tgggtttcta attttttgcg ccacttttcg
     2221 gcaaccgcat ccagattctc atatatccct tccagactct gaaactcgac cagcagctgc
     2281 gtggcgcttt ttggtccgat tcccgcaaca cccggtacct ttgaactgct gatccccgca
     2341 agtccccagt aatcgggcaa ctgctgcggt tgaacgccaa attctttatc gataaatggc
     2401 gcatccagcc aacgtttctg gaagtaatca cgaatacgta atgtcggtga aagtaactga
     2461 cagtagcctt tatctgtcga aacaatcgtt gcctgatgcc cggcctgtgt cactttgacc
     2521 gccagcgtgg cggctaagtc atcggcttcg ttgccgctgg ctgaccagca cgggacgccg
     2581 cgttgctcaa aggcggcgcg taatgcaggc atctcgtcgt gcaactcttc cggcattggc
     2641 ggtcgacccg ctttgtaatc tggtaaacgc tgatgacgcc agccgctact gcggttttca
     2701 tcatcaaaaa cggcgacagc gtgggttggc tggctgtgca taatgagctg atcgagcgcg
     2761 tgctggcagg tttcgacaca gggcgacccc tgaacggcat gaatgcgacg aataagattc
     2821 agtgcatcga caataagcaa atgaacagcc acgataaccc ccttaaagat caggcgataa
     2881 gggtaacatt cgatgccgga acaggctacg gctggatgag aaatcgggaa gaggcctcgc
     2941 aaaacgaggc ctctggagag cgattaatcg caggcaacga tcttcattgc caggccgccg
     3001 cgagaggttt cgcggtactt ggcgttcata tctttacctg tttcgtacat ggtttcgata
     3061 actttatcga ggcagacgcg cggctcgctg gtacggcgta gcgccatacg tgcggcgttc
     3121 accgctttta ctgccgcaat ggcgttacgc tcgatgcatg gcacctggac ctgtccggcg
     3181 accgggtcac acgtcagacc gaggttgtgc tccatggcga tttccgccgc gatgcacacc
     3241 tgcgccgggc ttgcgcctaa cagttctgcc agaccagccg ccgccattga gcacgccaca
     3301 ccaacttcac cctggcagcc cacttcggca ccagaaattg atgcgttcat cttataaaga
     3361 gaaccaatgg cgctggctac cagcaggtaa cgagccagtg agttagcgtt cacttcgcgg
     3421 ataaacttat cgtagtacgc cagcacagcc gggataatcc cgcacgcacc gttagtcggc
     3481 gcagtcacca cgcgaccgcc agcggcgttc tcttcgttca ctgccagtgc aaacatgttg
     3541 atccagtcaa ctaccgccat cgggtcagtg gtggttttat cctggctgac cagcatccgg
     3601 cgtagtgccg cagcacggcg tggaacgcgc agtttgccag gcaacacgcc ttcggtggaa
     3661 ataccgcgct caataccgcc gcgcatgact tcccagacgt tcgccaggtg ctgttccagc
     3721 tcttctttgc tgtgcagcgc cagctcgttt ttcatcatca ggccagagag tgacagcccg
     3781 gtttcctgac aatgtttttg cagatcggct gctgaactgt acggataagg aacttcaacc
     3841 ggtgcgctat cctgctggcc aaaatgctct tcatcaacga taaagccacc gccaatagag
     3901 tagtaagtct ggctgtaaac gactttatcg cccgccagcg cggtaatgcg cataccgttt
     3961 tcatgcagag aaaggttgtc ggcatggaag ttcatgcact gatcaaccgg gaactccact
     4021 tcatgctgac catttgccag catcaggcga ccatgagtat tcacatcctg aataaaacca
     4081 gggatggaat cgatatccac ggtatctggc aggttacccg ccaggcccat aataatggcg
     4141 atatcagtgt ggtggccttt accagtcaga gagagcgagc cgtacacgtc aaccaccacg
     4201 cgggtcacgt ctttaagcag gttacgggca atcagatcgt cggtaaattg tttacccgct
     4261 ttcattggtc caacggtatg agaactggaa gggccaatgc cgattttgaa aatatcgaat
     4321 acgctaatca taggaaatac atcgcgttaa aacggaggaa gcgccgcccg aaagcggcgc
     4381 gaaaggactt agctgaacag agagtagaag attgcggaga ttgcaatcag acccatcacg
     4441 acaacgaata cgttgctgat gtgaccgctg tacttacgca ttgccggtac tttctgaatt
     4501 gcgtacatcg gcatcaggaa caggatcatc gcgatgattg ggccgcccag ggtttcaatc
     4561 atacccagga tgctcgggtt cagggtggca acaatccagg tcgttaccag catgaacagc
     4621 gcagtgatac ggttcagctt gttgatttcg atagacttac ctttaccacg cagagattta
     4681 atcaccatac cgttgaagcc ttcacgtgcg cccaagtagt ggccgaggaa ggatttggtg
     4741 atagcgataa tcgcgataat cggagccatc cacgcgataa ccggtgcgtt aaagtggtta
     4801 gccaggtaag acagaatcga gatgttctgc tctttagccg cagccaggtc tgccggagtc
     4861 aggctcagta cgcagctgaa gacgaagaac attacggtca gcaccatcat gatgtgtgcg
     4921 aatgccagga tcttcgagca tttctgttct gccatatcgc cgtactcttc acgcttcgca
     4981 acggcgaaag aagagatgat cggagagtgg ttgaacgaga acaccattac cggaattgcc
     5041 agccacaggg tcatccacag accgtttccg gttgcagatg cagtgtccag agacagcgtt
     5101 tccagtgctg cgccgttcca ctgcgggatc aggtacagag ccagcagcat cagtacgcca
     5161 acaaacggga ataccagaat actcatcgct ttaacgatca tctgctcacc aaagcgaacg
     5221 atggtcatca tacccacgat caggatcagc gacagaatcg cacgcggcgg tggcgtcata
     5281 cccagctggt gagacatgaa gctttcaact gtattggtga ttgccacgct ataaaccagc
     5341 aggatcgggt agatagcgaa gaagtagagc agggtaatca gtttacctgc gccaatacca
     5401 aagtgttctt ctacaacctc ggtgatgtct tcgcccgggt ttttaccaga cagtacgaag
     5461 cgagtcaggc cgcggtgagc aaaaaacgtc atcgggaacg caaggatagc catgatgatc
     5521 agcgggatca taccgccaac accggcgttg attggcagga acagcacgcc cgcgccgatt
     5581 gccgtgccgt aaaggcccag catccacatg gtgtctgtct tgcgccaggc actacgagag
     5641 tctttcgacg caatcgtgct ggtttgagtc gtttccatct atttctcctg gaggaatgca
     5701 aataattcag cttttagcca gttcagcccg ggaaggtgcc tgaccgtaaa aatgaaatct
     5761 gtctatcggc gatggcttaa tcatttaccg ccgtaataat ttgagggcgg aaagatacac
     5821 acaagttatg tatctatcag tgatcttgat ctcaaataca ataatctact tggaagatag
     5881 attatcccaa cgctttactc tgtaaatcaa aagtaaaata gaatttaagg gcaatatttg
     5941 cccattaaac gacatctgac ttcttccggg tcgcgcaata tatgtaggtt gaactgaaaa
     6001 aatcaatact tttagttaat catgggctat gaaaa
//
LOCUS       DAZGXZ010000228         5898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000228 DAZGXZ010000000
VERSION     DAZGXZ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.229"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(7..228)
                     /gene="kilR"
                     /locus_tag="ACHKJF_005082"
     CDS             complement(7..228)
                     /gene="kilR"
                     /locus_tag="ACHKJF_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFP0952286.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIEKRKLYIR
                     NFTTKTCITDCMIKVFLGRDGLPVKAESW"
     gene            809..1063
                     /locus_tag="ACHKJF_005083"
     CDS             809..1063
                     /locus_tag="ACHKJF_005083"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952287.1"
                     /translation="MANIYSPVEYFKITNQNIKNIVATQQLFGKLLGVIAARTGLTNE
                     LKEIIESSAATEESMSEHVEYSKQFILNSMSLVRIIKDHS"
     gene            complement(1140..1262)
                     /locus_tag="ACHKJF_005084"
                     /pseudo
     CDS             complement(1140..1262)
                     /locus_tag="ACHKJF_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1264..1419)
                     /locus_tag="ACHKJF_005085"
     CDS             complement(1264..1419)
                     /locus_tag="ACHKJF_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0952288.1"
                     /translation="MDMLNLGNNESLVFGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LERNSGE"
     gene            complement(1693..2337)
                     /locus_tag="ACHKJF_005086"
     CDS             complement(1693..2337)
                     /locus_tag="ACHKJF_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP0952289.1"
                     /translation="MESLGIRLKKLRKDKGLTQVELGKLSGVTGVTIGYWEKDLNEPG
                     SKALSKLALALGTTESYLLYGVSSPESSLVQKNPGTQIPYLSWGEAISFLILKGEKTM
                     GNVDRITTFFDVKEGDFAVSMPDDTMHNPSGSPSIPVGATVIMRPGEKYKNGSIVAVI
                     VPDPLTNEPSMTIKKLVIDGKLVYLAPLNPRYQSSLLTPECNIVAVAKGVQFSL"
     gene            2437..2664
                     /locus_tag="ACHKJF_005087"
     CDS             2437..2664
                     /locus_tag="ACHKJF_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0952290.1"
                     /translation="MRVDELVQFFGSVQRVADFYGVTREAIYMWRKRPGEIVPKGRAA
                     EAVAYSKGKLSLNPELYKKKDNTSNERKNDS"
     gene            2661..3098
                     /locus_tag="ACHKJF_005088"
     CDS             2661..3098
                     /locus_tag="ACHKJF_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0952291.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYFELEMTYPPM
                     HDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLPRELRYHLSIFDTVER
                     RALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH"
     gene            3185..4195
                     /locus_tag="ACHKJF_005089"
     CDS             3185..4195
                     /locus_tag="ACHKJF_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFP0952292.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESNGAPLQTAEPEYLDGLSEPIGKFPMTGDWHPSPDFRRRAALWGVALPES
                     EFTPAELAAFRDYWAAEGKVFTQVQWEQKFARHVNHVRAQVKPVSRGVNHAAAAGTAS
                     RAVQEIRAAREQWERENGFVSDGNGMEAVGTHGRGVFEPLDSEERSCSVEALDCPDWR
                     DD"
     gene            4188..4649
                     /locus_tag="ACHKJF_005090"
     CDS             4188..4649
                     /locus_tag="ACHKJF_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0952293.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRSKGIERQMTEGELKRLVE
                     RQLTKWAKHVGNGLSVPPVRRQLAAPKRPPGPTPIELLKQEYERRKAAGFV"
     gene            4684..5397
                     /locus_tag="ACHKJF_005091"
     CDS             4684..5397
                     /locus_tag="ACHKJF_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0952294.1"
                     /translation="METVFDALKALKKASSHEIAARLEISRDDAVNELWKLKRRGEAD
                     NKGSMWWLTSEATEMATKTTAEMLINAIEQHGPQSADELALMFGITSRRANSTLAMAI
                     SKGRLIRVNQNGKFRYCLPGDNLPAEPKAASVTETAGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQPSFTRKHPDSLILPSLHVANRELRRAKGQVQKWERVCAALRELNK
                     HQDIVRQITDSSCCVASDK"
     gene            5413..5811
                     /locus_tag="ACHKJF_005092"
                     /pseudo
     CDS             5413..5811
                     /locus_tag="ACHKJF_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
BASE COUNT         1605 a         1300 c         1520 g         1473 t
ORIGIN      
        1 tactcattac catgattcag cttttaccgg caaaccatca cgtcccagga aaactttaat
       61 catgcagtcg gtaatgcatg tttttgtcgt gaagttacga atatagagtt ttctcttttc
      121 aatattgttt gctgaagcga tatatgtccg accttcatga agaacataat cgccaggcgt
      181 cacgcactga cgtggtattt catcagttcc gaagtgatga gcaatcataa ttatctccat
      241 tttcacaaat gaactttgtt gatgcagtgc ctggtgcctc taggtgacgt taaccagtta
      301 acaattaacg ccggaataca taagaggata cttacgccag taaaagaccg ctttactgat
      361 ttaactgttc cgcgtgcgct tagccgcatt caccgcatca caaaattcac tttaaaaagg
      421 gcggcagagc agtcacggag taaaactgat accgccaaac gtcaccagaa aattgataac
      481 agagggcgtt gcagcggggt tgtcacttaa gcgtatggtc aaccttacaa cccggtgtcc
      541 tcaacgggga agaaataacc ccgccatact taccgccgcg ccatttcgcg gattgccaca
      601 accggaagca cacggtcgaa gaaatctaac gacaagcctt ctaaggaaaa gacgccttcg
      661 ccgtgtgctt tcgtgttgtg ccctgacttt tcagggaaat atcctttcag taaactgtca
      721 gtgccggatg ctcacccgtg tccggtgcac gttctccccc tcaccagtgg ataactccaa
      781 aactactgac aaccccagga gggtgaatat ggcaaatatt tatagtcctg tcgaatattt
      841 caaaataact aatcaaaaca tcaaaaatat tgttgccact caacaactct ttggtaaatt
      901 acttggagta attgctgccc gcacaggttt gactaatgaa ctaaaggaga taattgaatc
      961 cagtgctgcg acagaagaat cgatgtctga gcatgttgag tattcaaagc agtttattct
     1021 caactcaatg tctttggttc gcataattaa agaccattct taacgcttaa gaacacttcg
     1081 tttatgaagc atccactcgc atctggtgat gttgtattta tcgtagttaa atcgcttttc
     1141 ttaatagcgc gtttagcaat ccagatgcgg gcctcagtac ctgcatttgg ttccacctgc
     1201 tggaggcgct ttgcatcttc cagaagcagg gcgataacgt gttttaattc tgcctcgttc
     1261 attttattca cctgagtttc tttccagcca gcgacgcgcg ccagcttcgg ttttaaacgt
     1321 tttgcttctg gtatacgtca tcgcggtgaa cgtaccgtcc tggtttggga acacgccgaa
     1381 aaccagagat tcattgttgc caagattgag catatccatg ttgacctcat ttacccttaa
     1441 cgccgggtag cggaactaaa aacctgctgc gctgttatac aaagtgttcc cgccgtcatg
     1501 ttcatacgcc tcgggctggc tacttaaccc ctgaccactg ccgggtaact cgaagtattg
     1561 ccctgcattc tgtgaagcgg ggtgggttgt aatgaatata agaatattta gttttgtcgt
     1621 caagttgaat ataaatattt ttaagataaa aggccggcaa agccgacctc tgattacagg
     1681 gaaaagcggg agctagaggc tgaactgaac accttttgct acagcaacga tgttacattc
     1741 cggtgtaagt aaggatgatt gatagcgtgg gttaaggggg gctaagtaca cgagctttcc
     1801 atcaataact aattttttta tagtcatgga tggttcgttt gtgagtggat ctgggactat
     1861 tactgcgacg atgctaccat ttttatattt ttcgccaggc ctcatgatca ctgtggctcc
     1921 aactggaatg cttggggatc ctgatggatt gtgcatagta tcatcaggca ttgaaacggc
     1981 aaaatctccc tccttgacat caaagaatgt ggtgatcctg tcgacatttc ccatagtttt
     2041 ctctcctttt aatattagga aagaaatcgc ttctccccaa gaaaggtatg ggatttgggt
     2101 acctggattt ttctgcacaa gggatgattc aggtgatgat actccataca ggagatagga
     2161 ctcagtagtg cctagtgcta gggctaattt actaagagct ttgctacctg gttcgtttag
     2221 atctttctcc cagtatccta ttgttacccc agttacgcca gaaagcttgc caagttcaac
     2281 ttgggtcaga cctttgtctt ttctgagttt cttaagccta atgccaaggc tttccattat
     2341 tttctcccgc gaattgaata taaattattt tagattgtat tgacctaaaa aaaattaccc
     2401 tttaatctaa aaatacttag tttttaaggg ggcgaaatgc gagttgatga acttgtccag
     2461 ttttttggtt ctgtccaaag agtcgctgat ttttatgggg taacccgaga agctatatac
     2521 atgtggcgta agcgtcccgg cgaaatagtt cccaagggga gggctgctga agcagttgca
     2581 tactccaagg gaaaattatc gttgaaccca gaactttaca aaaagaagga taacacctcg
     2641 aacgaaagga aaaatgattc atgaaaatca aacatgagca catccgcatg gcgatgaatg
     2701 cgtggctgct ttatccgagg gtagggcgca aaaaaatcgc tgatgatata gcgacagcat
     2761 attttgagct tgaaatgact tatccaccaa tgcatgacac ctctacgaca gagggtattg
     2821 gattgaacat acaaaatatt tttcgctggc ttgaaaagga tacgcctgat gctgttgaaa
     2881 aaattcaggc actaattccg gctatcctga ctgttcttcc gcgtgaactg cgttatcacc
     2941 tcagtatttt tgacactgtt gagcgccgtg cattactggc ggcgcaggaa gcgttgagta
     3001 cggcaattga tgcacatgat gatgcagtcc aggccgttta ccggaaagcg tatttcagcg
     3061 acggcgggtc atccggcgag tctgttgtgg tgcattgata tttatgccag acccctgctg
     3121 attctgttga ttggggaatc acagattatc accagaggat ggttcgtcac aagatgaggc
     3181 aattatggcc gcattaccat acatgcaact gtacatagct gattacctgg ctgacaccat
     3241 gcatttgtcc gcagaggagc acggtgcgta tttgttgctg atgttcaatt actggcaaac
     3301 agggaaacca atacctaaaa acaggctggc aaaaatttcc cgtctgacta acgagcgatg
     3361 ggctgatgtg gaaccatcct tgcgggagtt tttttgcgat aacggcgatg aatgggtgca
     3421 tcttcggatt gaggaagatc tggcatcagt cagggaaaaa ttgaccaaaa aatcagccgc
     3481 cggaaaagca tctgttcagg ccagaagaag cagaaaggaa gcatatgttc aaacaaaaca
     3541 agagagagat ttaacaggtg ttcaaacaaa tgttggtgtt gtgtttgaac atgacgcaaa
     3601 cacaaaagca actaataaag atacagatct aaaagaatta aaccccacac ataacgtgcg
     3661 tgaacgcgag agtattccga ccagtgagtc gaatggtgcg ccgttgcaga cagccgaacc
     3721 tgaatacctg gacggcctta gcgaacccat cgggaaattt ccgatgactg gtgactggca
     3781 tccgtcgccg gattttcgac gacgggctgc actgtggggc gttgccctgc cggagtcgga
     3841 atttacacct gctgaacttg ccgccttccg ggactactgg gcagcggagg ggaaagtttt
     3901 cacgcaggtt cagtgggagc agaaattcgc ccgtcacgta aatcacgtca gggcgcaggt
     3961 taaaccagtc agcagggggg tgaaccatgc ggcagcagct ggaaccgcat cacgggcagt
     4021 tcaggaaatt cgggcagcac gtgagcagtg ggaacgtgaa aacggatttg tcagcgacgg
     4081 aaacggcatg gaagctgtgg gaactcatgg gagaggtgta ttcgaaccgc tggactcaga
     4141 agaacggagc tgctccgtcg aagctctgga ttgcccagat tggcgcgatg actgaacagc
     4201 aaatccggca ggtatgccgc cagtgcatgg accgttgccg ggctggtgaa acatggcctc
     4261 ctgacctggc tgagtttgtt gcgctgattt cggaaagtgg ggcgaatcca tttggtctta
     4321 cggtggatgc cgtgatggag gagtaccgtc gctggcgcaa tgaatcctgg cggtacgacg
     4381 ggagtgataa atacccgtgg ccacagcctg tgctgtacca catttgcctc gagatgcgtt
     4441 caaaggggat tgagcgccag atgaccgaag gggagttaaa acggcttgtg gaaaggcagc
     4501 tgacgaaatg ggcaaagcat gttggtaatg gcctcagtgt tccgccagtc cggcgacaac
     4561 tggcagcacc caaacgcccg ccagggccaa cgccaattga attgctgaaa caggaatatg
     4621 agcgccggaa ggcggccggt tttgtctgag ttgagaagta atttttaccg ggaggaaaat
     4681 ttaatggaaa ccgtatttga cgcactgaaa gcactgaaaa aagcctcgtc gcacgagatt
     4741 gcagcccgtc ttgaaatcag ccgtgacgat gctgtcaacg agctgtggaa gctgaaacgc
     4801 cgtggtgaag ctgataacaa ggggtcgatg tggtggctga cgagtgaggc aactgaaatg
     4861 gccacaaaaa ccactgcgga gatgctgatt aacgcaattg aacagcatgg tcctcagtcg
     4921 gctgacgaac tggcgttaat gttcgggatt acctcccgcc gggcgaattc aacgctggca
     4981 atggcaataa gcaaaggtcg tctgattcga gtaaatcaga acggtaaatt tcgttactgc
     5041 ctgccgggcg ataatttacc agcagagccg aaagctgcat cggtaacgga aactgctggt
     5101 aaagcctttc ctcagccagc aggtgttgcg ttaccagtcc gggaagcgga aacacaggaa
     5161 gaaattaaaa ctgaaagtgt ggcggtcaca gtgcagtcac agccgtcgtt caccagaaag
     5221 catccggata gtctgatttt accatcgctg catgtggcta accgcgaact gcgccgggca
     5281 aaaggtcagg ttcagaagtg ggagcgagtc tgtgctgcgc tgcgtgagct gaacaagcac
     5341 caggatattg ttcgacagat tactgattca tcctgttgtg ttgcatcaga caagtgattg
     5401 caggggacgc tgatggcaag agtatttaca ccagaagagc gggaaaaaat taaggggcag
     5461 gttgttgaac tcgtacgcca gagcggtcgc gagacgttac ggcaactgga agctaaaaca
     5521 ggtgcaacaa gatatctggt gatcgttctc gccagagagc tggttgccag tggtgatgta
     5581 tacaactctg gctacgggtt atttccgtct gaacaggctc gtaaagactg gcaaaacgcc
     5641 cgcaaaaaac tttcaaggga aaaggcgaag aaaccggctg tggttgatcc ggaccttatc
     5701 tggtcattac cagacggaga aatacgccgc tacgacaggc gcctgaacat aatctgtcgc
     5761 gagtgccgga atagtgaagt tatgcagcta gtgctggcgt tttatcaggg ggtggggtca
     5821 ggaggtggtg ctgtgagtga aattagctat caggcttcaa tttccgctgg cattcgcatc
     5881 aaaggagagg agcatgga
//
LOCUS       DAZGXZ010000229         5888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000229 DAZGXZ010000000
VERSION     DAZGXZ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.230"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1239)
                     /locus_tag="ACHKJF_005093"
     CDS             complement(<1..1239)
                     /locus_tag="ACHKJF_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020315086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4209 domain-containing protein"
                     /protein_id="HFP0952295.1"
                     /translation="MDDINISLDELNTIDLSPLIQSKLPVTCFDITKYLSSLGDGRAE
                     IRLLTHICGFHFRPENPQVPFGPCFQSSQGRSAIPDDINGLSLEVLSQFCPTIELPEL
                     QARIADTLWVRKIGGIRFPLLAVRAYYASSIAIMTSQGTWVSALERLERALRLCCFFR
                     KHTDFRDEFDQLSAHLLAEYERTREQGDSPYPLRLLQLDIDCKVSEPSFIAQELLFLT
                     KNYLAQKLFSFAVDACKTAIPIANRCCDRDTQFEFWRLLADTHLEESKFQDGGMISAA
                     CMQNAIEALANIPGTRIERLALYEEMRDYQIESRHQMSILQSPPQDISEIVHQAKSRV
                     VGRDLFDMVFRLAMLVCRPTSIERLKAQAIEQKANSIAWMFGSTHIDHEGMTLARIPA
                     GLGIDDANGAVIWPIMMTRMR"
     gene            1914..2069
                     /gene="hokD"
                     /locus_tag="ACHKJF_005094"
     CDS             1914..2069
                     /gene="hokD"
                     /locus_tag="ACHKJF_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0952296.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            2286..2537
                     /gene="rem"
                     /locus_tag="ACHKJF_005095"
     CDS             2286..2537
                     /gene="rem"
                     /locus_tag="ACHKJF_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFP0952297.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNLVSHYRIMCLRKNGAALIN
                     FVDGRVTDGFILREGEFVTSLQALKEIGIKAGFLAFAEE"
     gene            2604..2882
                     /locus_tag="ACHKJF_005096"
     CDS             2604..2882
                     /locus_tag="ACHKJF_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952298.1"
                     /translation="MARNVKYYNSDNSPVLVCTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            2884..3933
                     /gene="ydfU"
                     /locus_tag="ACHKJF_005097"
     CDS             2884..3933
                     /gene="ydfU"
                     /locus_tag="ACHKJF_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0952299.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQSLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLELLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKTPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHTDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            3947..4699
                     /gene="ydfT"
                     /locus_tag="ACHKJF_005098"
     CDS             3947..4699
                     /gene="ydfT"
                     /locus_tag="ACHKJF_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0952300.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDAQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIGKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(4874..5076)
                     /gene="cspF"
                     /locus_tag="ACHKJF_005099"
                     /pseudo
     CDS             complement(4874..5076)
                     /gene="cspF"
                     /locus_tag="ACHKJF_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            5375..5590
                     /gene="cspB"
                     /locus_tag="ACHKJF_005100"
     CDS             5375..5590
                     /gene="cspB"
                     /locus_tag="ACHKJF_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFP0952301.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAANVIITD"
BASE COUNT         1629 a         1254 c         1449 g         1556 t
ORIGIN      
        1 acgcattctc gtcatcatta tgggccaaat aactgcccca tttgcatcat caatccccaa
       61 tcctgcgggt atgcgagcaa gtgtcattcc ctcatgatca atatgtgttg atccaaacat
      121 ccaagctatg ctgttggcct tttgttctat tgcttgagcc ttaagtctct caatgctggt
      181 tggccgacag accagcatcg caagtctgaa aaccatatca aacaaatctc ttccgactac
      241 ccgactcttg gcttgatgga caatctcact aatatcttga gggggagact gaaggataga
      301 catctggtga cgggattcga tttggtaatc acgcatttct tcatacagcg caagacgttc
      361 tattcgggtt cctggaatat tagccagtgc ttcaatagca ttctgcatgc aggctgccga
      421 aatcataccg ccatcttgga attttgactc ttccagatgt gtatcggcaa gaagccgcca
      481 gaattcaaac tgtgtatctc tgtcacagca cctattcgca atcgggattg cggttttaca
      541 ggcatcgacg gcaaatgaaa ataatttttg agccagatag ttttttgtca gaaacaggag
      601 ttcctgggca ataaaagatg gctcactcac tttacagtcg atatcgagtt gcaggagtcg
      661 cagcggataa ggtgaatcgc cttgttctct ggtacgttca tactctgcta gtagatgtgc
      721 cgaaagctga tcaaattcat ccctgaaatc agtatgcttt cgaaaaaaac aacacagcct
      781 aagcgcacgc tctaggcgtt ctaaagcact aacccacgtc ccttgcgatg tcataatagc
      841 tatactggac gcataataag cacggactgc cagcaaagga aaacggattc caccaatttt
      901 acgaacccaa agtgtatcag caatacgagc ttgaagttcg ggtagctcta tcgtcgggca
      961 aaattgagaa agtacctcca gcgacaaccc gttaatgtca tccggaattg cagatctacc
     1021 ttggctgctt tgaaagcacg gaccaaatgg tacctgtgga ttttcgggac gaaagtgaaa
     1081 accgcaaata tgggttagca gtcttatctc cgctcttcca tcacctaggc tagaaagata
     1141 ctttgtgata tcaaagcagg taactggcag cttactctgg attaaggggg ataaatcaat
     1201 agtgttaagt tcgtcgaggc tgatattaat atcgtccatg tcgtcacctt gatgatttgt
     1261 taatgcgggg gcaacattaa taaatgtact tgagaccgca atttgtcgat ggagccaaca
     1321 tttataacta tgtgcttcta ccctctgaat ttttacatgt gaattgctag cttgaatagc
     1381 tctatcatta tatacgtggc actaaattat ggctgttttt cgctcaaagc ggactagaag
     1441 gttagcttgt gtcggacttt gcgtattaaa agaagtgctg gtggtgacta gtggttgtgc
     1501 cccatttcca cagaaaaaat cagagaaact atacccaata gttgtattga atcactgacg
     1561 agacagcctc atattcatca ggactggtct acgtccaata caggaggttg tggtgctggt
     1621 tctcaaatgt gcgctggcta ttacggctgt aatggcgatt tattgtcttg ctattgttct
     1681 tatggatcgc ctttctgact gatttcacat tggcgaggta acggtagtta agtagaatgg
     1741 ctgcgggtgc ttgaggctat ctgcctcggg catgaacacc aacggcagat agagaaaagc
     1801 cccagttaac attacgcgtc ctgcaagacg cttaacatta atctgaggcc aatttcatgc
     1861 tagacatatg taggttagcc tcttacgtgc cgaaaggcaa ggagaagcag gctatgaagc
     1921 agcaaaaggc gatgttaatc gccctgatcg tcatctgttt aaccgtcata gtgacggcac
     1981 tggtaacgag gaaagacctc tgcgaggtac gaatccgaac cggccagacg gaggtcgctg
     2041 tcttcacagc ttacgaacct gaggagtaag aaacccggcg agggagaaat ccctcgccac
     2101 ctctgatgtg gcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc gggtttttgt
     2161 ttttattttc aacgcgtttg aagttctgga cggtgccgga atagaatcaa aaatacttaa
     2221 gtagcgcgca gggataagag ggatggtccc ttaaagggga gagctaatta tccggaagga
     2281 ttctgatgat gaacatcgaa gaattgcgta aaattttttg tgaagatggc ctctatgctg
     2341 tgtgcgttga aaatggaaat cttgttagtc attaccgcat tatgtgtttg cgaaagaatg
     2401 gggctgcgtt aattaatttt gtggatggtc gagtgacaga cggatttatc ttgcgcgaag
     2461 gtgagtttgt cacttcatta caggcactga aagagatcgg aataaaagca ggctttttag
     2521 cttttgcaga agaataaact catctacaat cttgcgcggg gctgaactcc cgctgagtaa
     2581 caccgtgcca ccggagaaaa ccgatggcac gcaacgtaaa atattacaat tctgataatt
     2641 cgcccgttct tgtctgcacg cacgagcggt attctcacgc attcaagtct gaatggttcc
     2701 aacaccctcc atgcactgaa gagcaggctg aatggataat tcagtgttac cgcaggcgcg
     2761 gatacgaggt taagaaagcc cttagcctcg actaccgtca ctggataatc tcagtcagat
     2821 tgccttactc cgaacgccca ccacgtgcgt cccgcacttt ccagcaacgg atctggaggt
     2881 aacgtgcggg tattacttag acctgttctg gtgcctgagc ttgggctggt ggtccttaag
     2941 ccgggccgtg aatccataca gatatttcat aatcctcgag tgctggtgga accggaacca
     3001 aaaagcatgc gtaatctgcc atccggagtc gttcctgccg ttcgccagtc gctggcggaa
     3061 gacaaaacat tgctgccgtt ttttagtaac gaacgggtga ttcgtgctgc tggcggtgtt
     3121 ggcgcattgt ctgactggct attacgtcat gttacatcct gccagtggcc taatggcgat
     3181 taccatcact ctgaaacagt cattcaccgt tatggtaccg gcgcaatggt gttgtgttgg
     3241 cactgcgaca accaactgcg tgaccagaca tcggaatcac tggagctgct tgctcaacaa
     3301 aatctgacag catgggtgat tgacgtcatc cgtcacgcaa taagcggtac gcaggagcgg
     3361 gaattatctt tggctgaatt atcctggtgg gcggtctgca atcaggtggt ggatgcacta
     3421 cctgaggctg tatcgcgtcg ttcgctggga ttaccagcgg aaaaaatctg ctcggtgtac
     3481 cgcgaaagcg acatcgtacc gggagagcag accgccacca gcatattgaa acaacgcaca
     3541 aaaaatcttg caccgttgcc ttacgcccac cagcaacaaa aaacaccaca ggaaaagacg
     3601 gtggtaagca tcaccgttga tccagagtct ccggaatctt tcatgaagct gcctaaacgt
     3661 cgccgctggg ttaaggagaa atacacacgt tgggttaaga cacagccgtg tgcttgctgc
     3721 ggtatgccag ccgacgatcc gcatcatctg attggtcacg ggcagggcgg aatgggaaca
     3781 aaagcacatg atctctttgt gttgcctttg tgcagaaagc atcacaacga gctgcatacg
     3841 gatacagtgg catttgaaga gaagtatggc tcccaactgg agctgatatt tcgttttatc
     3901 gatcgcgcgc tggcaattgg cgtactggcg taagtggaga acgagcatga accttgaagc
     3961 cttaccaaaa tattactccc caaaatctcc aaaattgagc gatgacgctc cagcgacagg
     4021 caccggttgt ttaacaatta cggatgtaat ggcagcgcag gggatggtgc agtcgaaagc
     4081 accacttggg ttggccttat ttctggcaaa agttggtgtt caggacgctc agtttgcgat
     4141 tgaaggcctg ctaaattacg cgatggcact ggataacccg acattgaaca aattgagtga
     4201 agaaatccgg ttacagatta ttccttacct cgtgaatttt gcctttgctg attactccag
     4261 gtctgcggca agtaaggctc gctgtgagca ttgttcaggt acgggatttt ataatgtatt
     4321 gcgcgaagtg gtgaaacact acagacgcgg ggaatctgta atcaaggaag aatgggtgaa
     4381 ggaactatgt cagcattgcc atggtaaggg cgaagtcagc acagcgtgca gagggtgtaa
     4441 gggtaaaggg attgttctgg atgaaaaaag aacccggttt catggcgtac cggtatataa
     4501 gatttgtggg cgttgtaatg gaaaccggtt tagtcgttta ccgaccacgc tggcacgacg
     4561 tcatgtccag aagctggtac cagacctgac cgattatcag tggtataagg ggtatgcgga
     4621 cgtcattggt aaactggtaa caaagtgctg gcaggaagaa gcatacgcgg aagcgcaatt
     4681 gaggaaggtg acgagataaa tgatttttgc tgaagatggc gacatgatgt ttgcattttt
     4741 caaaaaacat ggataagatt ttctcaacga tgggctttgt gtatccgacg tttagaaaaa
     4801 agtagaaaac ccgcttataa gcgtgttttt gtgctttaaa tggggcaata gagatattga
     4861 atctcatccc gggataaaca ttggcagttg aaggtccacg cgaaccattt atccagcaaa
     4921 attccacgcg taatcctgtg gtaatttctt ctgcatctcg aagattgaga gctgaaacgt
     4981 gaagctgggc atcgatacgc catcggatgg gaatataaga cctttgctgc ttttgtagtc
     5041 aaagtttttg acaattcctg tcattttagg ggacagaaaa actccttaat actgataacc
     5101 tggtgcacca tacacacgtt cctggagaaa actacttttt tgataggatt gaaggtggct
     5161 ggatgtctaa aataaacatt gcttcatatg ttcaactatg agttaatgac tgcgtcggtt
     5221 tgaagaacag acgatatacg aagtagttta ctaaagcagt tctcatttca ggtgttattc
     5281 acttattcct tctttgagtc tctccaatta agtacgaagt cgtttctgtt atgcaaacca
     5341 tttatgccga aaggctcaag ttaaggaatg tagaatgtca aataaaatga ctggtttagt
     5401 aaaatggttt aacgctgata aaggtttcgg ctttatttct cctgttgatg gtagtaaaga
     5461 tgtgtttgtg catttttctg cgattcagaa tgataattat cgaaccttat ttgaaggtca
     5521 aaaggttacc ttctctatag agagtggtgc taaaggtcct gcagcagcaa atgtcatcat
     5581 tactgattaa aattcatcgc tcgtctgtat acgataacga agaaggctga tgcctgagta
     5641 gagatacgga cagagtagtg aatattggat ctctttaata aaaagtaagg aggtccaata
     5701 catgaaacaa tggctagcat atttggcaaa atcttaatca ggaaaagtat gctaaccatt
     5761 gtggtgaagt gcaggtttgc tgcatgaata gtttcacagc agaagctaac tgctggcatc
     5821 gcaaaacaaa gtgcgtaagt ggatgactcc cacaaaaagc accacaatct caaacccgct
     5881 caggcggg
//
LOCUS       DAZGXZ010000230         5871 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000230 DAZGXZ010000000
VERSION     DAZGXZ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.231"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..1226
                     /gene="ompC"
                     /locus_tag="ACHKJF_005101"
     CDS             135..1226
                     /gene="ompC"
                     /locus_tag="ACHKJF_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0952302.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSLAYLQSKGKNLGRGYDDEDILKYVDVGATYYFNKNMSTYVDYK
                     INLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1338..2393
                     /gene="apbE"
                     /locus_tag="ACHKJF_005102"
     CDS             1338..2393
                     /gene="apbE"
                     /locus_tag="ACHKJF_005102"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP0952303.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQTAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            2467..3531
                     /gene="ada"
                     /locus_tag="ACHKJF_005103"
     CDS             2467..3531
                     /gene="ada"
                     /locus_tag="ACHKJF_005103"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP0952304.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADSEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3531..4181
                     /gene="alkB"
                     /locus_tag="ACHKJF_005104"
     CDS             3531..4181
                     /gene="alkB"
                     /locus_tag="ACHKJF_005104"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP0952305.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILWRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     ATTAAGYPDFQPDACLINRYAAGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4257..>5871
                     /gene="yojI"
                     /locus_tag="ACHKJF_005105"
     CDS             4257..>5871
                     /gene="yojI"
                     /locus_tag="ACHKJF_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP0952306.1"
                     /translation="MELLVLVWQQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAA"
BASE COUNT         1386 a         1483 c         1604 g         1398 t
ORIGIN      
        1 gttttagtat catattcgtg ttggattatt ctgcattttt ggggagaatg gacttgccga
       61 ctgattaatg agggttaatc agtatgcagt ggcataaaaa agcaaataaa ggcatataac
      121 agagggttaa taacatgaaa gttaaagtac tgtccctcct ggtcccagct ctgctggtag
      181 caggcgcagc aaacgctgct gaagtttaca acaaagacgg caacaaatta gatctgtacg
      241 gtaaagtaga cggcctgcac tatttctctg acaacaaaga tgtagatggc gaccagacct
      301 acatgcgtct tggcttcaaa ggtgaaactc aggttactga ccagctgacc ggttacggcc
      361 agtgggaata tcagatccag ggcaacagcg ctgaaaacga aaacaactcc tggacccgtg
      421 tggcattcgc aggtctgaaa ttccaggatg taggttcttt cgactacggt cgtaactacg
      481 gcgttgttta cgacgtaact tcctggaccg acgtactgcc agaattcggt ggcgacactt
      541 acggttctga caacttcatg cagcagcgtg gtaacggttt cgcgacctac cgtaacactg
      601 acttcttcgg tctggttgac ggcctgaact ttgctgttca gtaccagggc aaaaacggta
      661 gcgtaagcgg cgaaggcatg actaacaacg gtcgtgaagc actgcgtcag aacggcgacg
      721 gcgttggcgg atctatcact tatgattacg aaggcttcgg tatcggtgct gcagtttcca
      781 gctccaaacg tactgatgat cagaacagcc cgctgtacat cggtaacggc gaccgtgctg
      841 aaacctacac tggtggtctg aaatacgacg ctaacaacat ctacctggct gctcagtaca
      901 cccagaccta caacgcaact cgcgtaggtt ccctgggttg ggcgaacaaa gcacagaact
      961 tcgaagctgt tgctcagtac cagttcgact ttggtctgcg tccgtccctg gcttacctgc
     1021 agtctaaagg taaaaacctg ggtcgtggct acgacgacga agatatcctg aaatatgttg
     1081 atgttggtgc gacctactac ttcaacaaaa acatgtccac ctatgttgac tacaaaatca
     1141 acctgctgga cgacaaccag ttcactcgtg acgctggcat caacactgat aacatcgtag
     1201 ctctgggtct ggtttaccag ttctaatctc gattgatatc gaacaaaggg cctgcgggcc
     1261 cttttttcat tgttttcagc gtacaaacac agttttttgg tgtactcttg cgaccgttcg
     1321 catgaggata atcacgtatg gaaataagct ttacccgcgt ggcactgctg gctgccgcgc
     1381 tcttctttgt tggttgcgat caaaaaccac aaaccgccaa aacccacgct actgaagtta
     1441 ccgttcttga aggcaaaact atgggtacct tctggcgtgc cagcatcccg ggcattgacg
     1501 ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca gctggacgcc gacgatcagc
     1561 tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt taacgattcg caaagtttat
     1621 cgccgtggcc ggtaagtgaa gcgatggccg atatcgtcac cacctcgctg cgcattggcg
     1681 caaggaccga tggtgcgatg gatataaccg tcgggccgct ggtgaatctg tggggctttg
     1741 gcccggaaca acagccggtt caaattccga gccaggaaca gatcgatgcg atgaaagcca
     1801 aaaccggctt acagcacctg acggtcatta atcagtcgca tcagcaatat ctgcaaaaag
     1861 acctaccgga tttatatgtc gatctctcca ccgtcggtga aggttatgcg gcggatcacc
     1921 tggcacgctt gatggagcag gaagggattt cccgctatct ggtgtcggtg ggcggcgcgc
     1981 tgaacagccg tggtatgaac ggtgaaggcc tgccgtggcg ggtagcgatt caaaaaccaa
     2041 ccgataaaga aaacgcggtt caggctgtgg tggatatcaa cggtcatggt attagcacct
     2101 ctggcagtta ccgtaactat tacgaactgg acggcaaacg tctttctcat gttatcgatc
     2161 cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt gacggtgatt gctccgacgg
     2221 cgctggaagc cgatgcctgg gatactggct tgatggtact cgggccggag aaagccaaag
     2281 aggttgttcg ccgggaaggg ctggcggttt atatgatcac caaagaaggc gatagcttta
     2341 aaacctggat gtcaccacag tttaaaagct tccttgtcag cgaaaaaaat taaagcgcaa
     2401 gattgctggt ttttgcgtga tggtgaccgg gcagactaaa ggctatcctt aaccagggag
     2461 ctgattatga aaaacgccac atgcttaact gacgatcaac gctggcaatc tgtcttagcc
     2521 cgcgacccga atgccgacag cgaattcgtt ttcgccgtgc gcaccacagg catcttttgc
     2581 cgtccgtctt gccgcgccag acatgctttg cgggaaaacg tctccttcta cgcaaatgcc
     2641 agcgaggcac tcgccgctgg ctttcgcccc tgcaaacgtt gtcagccaga caaagccaat
     2701 gcccagcaac atcggttgga taaaattacc cacgcctgtc gactgctgga acaggaaacg
     2761 cctgtaacgc tggaagcctt agccgaccat gtggcgatga gtccgttcca tctgcatcgg
     2821 ttgtttaaag cgactaccgg aatgacgcct aaagcctggc aacaggcctg gcgcgctcgc
     2881 cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga cgtctattct taacgccgga
     2941 ttccccgaca gcagcagtta ctaccgcaaa gccgacgaaa cgctgggcat gacggctaaa
     3001 caattccgtc acggtggcga aaatctggcg gtgcgttacg cgctggctga ttgtgagctg
     3061 ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg cgatattgct gggcgatgat
     3121 gacgcgacac taatcagcga gttgcagcag atgtttcccg ctgccgacaa cgcgcctgcc
     3181 gatctgatgt ttcagcaaca tgtgcgtgaa gtgatcgcca gcctcaatca acgcgatacg
     3241 ccgctgacgt taccgctgga cattcgcggc actgcttttc agcaacaagt ctggcaggca
     3301 ctgcgcacga taccttgcgg tgaaaccgtc agttatcagc aactggctaa cgccatcggc
     3361 aaaccgaaag cggtacgggc cgttgccagc gcctgtgccg ccaacaagct ggctatcata
     3421 ataccctgtc atcgggtggt ccgtggtgat ggcacacttt ccggctaccg ctggggcgtg
     3481 tcgcgtaaag cgcaactgct gcgccgcgaa gctgaaaatg aggagaggta atgttggatc
     3541 tgtttgccga tgctgaaccg tggcaagagc cactggcggc tggtgcggta attttatggc
     3601 gttttgcttt taatgctgcg gagcaactaa tccgcgatat taatgacgtt gccagccagt
     3661 cgccgtttcg ccagatggtc acccccgggg gatataccat gtcggtggcg atgaccaact
     3721 gtgggcatct gggctggacg acccatcggc aaggttatct ctattcgccc attgatccgc
     3781 aaacaaataa accgtggccc gccatgccac aaagctttca tgatttatgt caacgtgcga
     3841 ctacggcggc gggctatcca gatttccagc cagatgcttg tcttatcaac cgctacgctg
     3901 ctggcgcgaa actgtcgctg catcaggata aagacgaacc ggatctgcgc gcgccgattg
     3961 tttctgtttc tctgggctta cctgcgattt ttcaatttgg tggcctgaaa cgtaatgatc
     4021 cgctcaaacg tttgttgttg gaacatggcg atgtggtggt gtggggcggt gagtcgcggc
     4081 tgttttatca cggtattcaa ccgttgaaag cggggtttca tccactcacc accgactgcc
     4141 gctacaacct gacattccgt caggcaggta aaaaagaata aaaataagaa ttattattgc
     4201 tgtgcgcgcg aagatgttta aactgcgggc tgtaattcat tgtccgggtt ttctgcatgg
     4261 aacttcttgt acttgtctgg cagcagtatc gctggccatt tatcagtgtg atggcgctaa
     4321 gcctcgccag tgcggcatta ggcattggct taattgcttt tatcaatcag cgccttatcg
     4381 aaacggcgga taccagtctg ctggtgttgc cggagtttct gggattattg ctgctgttga
     4441 tggcagtcac tctcggatcg caactggcgc tcaccacttt ggggcatcac ttcgtttacc
     4501 gactgcgtag tgaatttatc aagcggattc tggataccca cgtcgagcgc attgaacaac
     4561 tcggtagcgc ctcgttgctg gcgggattaa ccagcgatgt gcgcaatatc accattgctt
     4621 ttgtgcgtct tccggaactg gtgcagggga tcattctcac tatcggttcc gcggcgtatc
     4681 tgtggatgct gtcgggcaaa atgttgctgg taacggctat ctggatggcg atcaccatct
     4741 ggggtggttt tgtactggtg gcgcgggtgt acaaacatat ggcgaccctg cgtgaaaccg
     4801 aagacaagct gtacacggat tttcaaaccg tactcgaagg gcgcaaagag ctgactctga
     4861 accgggaacg cgccgagtat gtgtttaaca acctctacat ccccgatgcg caagagtatc
     4921 gccatcatat tattcgcgca gacaccttcc atcttagcgc cgtgaactgg tcaaacatca
     4981 tgatgctggg cgctatcggc ctggtgttct ggatggcgaa cagtctcggt tgggctgata
     5041 ccaacgttgc tgcgacctat tcgttgacgc tgttattcct gcgtacgccg ctgctttcgg
     5101 cggttggcgc attgccgacg ctgctgacgg cgcaggtggc gtttaacaag ctgaacaaat
     5161 tcgcgctcgc gcctttcaaa gctgagtttc cgcgcccgca ggcttttccc aactggcaaa
     5221 cgctggagct gcgtaacgtg acgtttagct atcaggataa cgcgttttcc gttgggccga
     5281 ttaacctcac cattaaacgt ggcgagctgc tgtttctgat tggtggcaac ggtagtggaa
     5341 aatcgacgct ggcgatgttg ctgacgggct tgtaccagcc acaaagcggc gaaatcttgc
     5401 tggatggcaa acctgtcagt ggcgaacaac cggaagatta tcgcaaactg ttttcggcag
     5461 tgtttaccga tgtctggctg tttgatcaac tgctggggcc ggagggtaaa cccgctaacc
     5521 cgcaactggt tgagaagtgg ctggcgcagc tgaaaatggc tcataagctt gagttaagca
     5581 acgggcgtat tgttaacctt aagttatcaa aagggcagaa aaaacgcgtg gcgctgttgc
     5641 tggcgctggc agaagaacgc gatattatcc tgctggatga atgggcggcg gatcaggatc
     5701 cacacttccg tcgtgagttt tatcaggtgt tgctgccgct gatgcaggag atgggtaaaa
     5761 ctattttcgc tatcagtcat gatgatcatt actttatcca cgccgaccgc ctgctggaaa
     5821 tgcgcaatgg gcaacttagc gagctgacgg gcgaagagcg cgatgccgct t
//
LOCUS       DAZGXZ010000231         5869 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000231 DAZGXZ010000000
VERSION     DAZGXZ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.232"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(58..444)
                     /gene="ridA"
                     /locus_tag="ACHKJF_005106"
     CDS             complement(58..444)
                     /gene="ridA"
                     /locus_tag="ACHKJF_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP0952307.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(517..978)
                     /gene="pyrI"
                     /locus_tag="ACHKJF_005107"
     CDS             complement(517..978)
                     /gene="pyrI"
                     /locus_tag="ACHKJF_005107"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP0952308.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(991..1926)
                     /gene="pyrB"
                     /locus_tag="ACHKJF_005108"
     CDS             complement(991..1926)
                     /gene="pyrB"
                     /locus_tag="ACHKJF_005108"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP0952309.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(1930..2064)
                     /gene="pyrL"
                     /locus_tag="ACHKJF_005109"
     CDS             complement(1930..2064)
                     /gene="pyrL"
                     /locus_tag="ACHKJF_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP0952310.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(2345..2740)
                     /locus_tag="ACHKJF_005110"
     CDS             complement(2345..2740)
                     /locus_tag="ACHKJF_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0952311.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(2871..3584)
                     /gene="bdcA"
                     /locus_tag="ACHKJF_005111"
     CDS             complement(2871..3584)
                     /gene="bdcA"
                     /locus_tag="ACHKJF_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0952312.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMSRGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3655..4248
                     /locus_tag="ACHKJF_005112"
     CDS             3655..4248
                     /locus_tag="ACHKJF_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0952313.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            4393..4845
                     /gene="tabA"
                     /locus_tag="ACHKJF_005113"
     CDS             4393..4845
                     /gene="tabA"
                     /locus_tag="ACHKJF_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP0952314.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            4968..>5869
                     /locus_tag="ACHKJF_005114"
     CDS             4968..>5869
                     /locus_tag="ACHKJF_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0952315.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFPQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEIDDLNAVNNYLLNNKKATDN
                     EINDIKVNLSHILIASLDDAKVNLT"
BASE COUNT         1538 a         1333 c         1434 g         1564 t
ORIGIN      
        1 attttgtgtt tgtcatcaga cttaatccgg gcatgatagc ccggatttcc atcaagatta
       61 gcgacgaaca gcgatcgctt cgatctcaat tttcacgtct ttcggcagac gggcaacttc
      121 aacgcaagaa cgtgccggga aggtggcatt gtgttcggtg aagaaggctt cgtaggtagc
      181 attaacggtt gcgaagtcgt tcagatcttt cacaaacacg gtggttttaa cgatgtcgcc
      241 cactttcagg cctgcggctt cgacgatcgc ttttacgtta tccagcgact gacgtgcctg
      301 tgcagcgacg tctgccggta cttcgcccgt tttcggattt accgggattt gaccggaggt
      361 gatgatcata ttgcccagat caacgccctg tacgtaagga ccgatagctg ccggtgcatt
      421 ttccgtcgcg atagttttgc tcatgatttc tcctttatta cagcggtaaa aagtctggct
      481 cattataggg agccagactt ttattaccaa ccgcaattaa ttggccagca ccacattatg
      541 ggaaaactct ttttcacagt atttgcattt gagcgcgatg tcattggcgc gttttcgcac
      601 ggcaaagctg gaagaaaccg gttcggcatg gctgatacag ttgctgttcg ggcagaccag
      661 cacattgtcg atgcgctctg gcagactggg gcgcgattta cccaccactt catagttgtc
      721 gatacggtta accgtggctt gcggcgcata caatgccagt tgatctacct gatcttcact
      781 caaaaaggta ttttcgattt tgatcagatc tttgcggccc atctcgccag aaggcaagtt
      841 cagaccgatg gtgatgcgct gatccgtttc ggtcagcttg aacagactca acagcttaaa
      901 accgatctgg gcggggatat ggtcaattac cgtgccgcgt ttaatagctt caacctgcaa
      961 tttattatcg tgtgtcatct ctatttcccc ttacagtacc agatcgcgat tcagaaccag
     1021 tgccagtaac gcctggcgag cgaaaatccc gttgcctgcc tgctggaagt accaggcgtg
     1081 tggcgtttta tcaacatccg tcgcaatctc atcaacacgc ggcagcggat gcagcacttt
     1141 catattggct ttggcgttgt ggagatcgct ggcgcgaaga acaaactgcg ctttcacgtt
     1201 ggcgtactcg gacgggtcca gacgctcttt ttgcacgcgg gtcatgtaca ggatgtctac
     1261 ttccgccatc acttcttcaa tagagctgtg cagactccat gcgatccctt tttcatcgag
     1321 catatccaga atgtattgcg gcattgccag cgcgtccggc gcgatgaagt aaaaacggtt
     1381 gccgtcgaac ttcgctaacg cctgagtcag ggagtgaacg gtgcggccat atttcaggtc
     1441 accaaccatt gcgacgtgga gattgtccag acgcccctgg gtttcctgaa tagtgaataa
     1501 gtccagcaag gtttgcgtcg gatgttggtt ggagccatca ccggcattca gtaccggtac
     1561 attgccggaa aactcggtgg ccaggcgcgc cgcaccttcc tgcggatgac gcatcactat
     1621 cgcatcgacg taagtgctga taaccgaaat ggtatcggcc agcgtttcgc cctttttacc
     1681 cagtgatgta ttggcgctgt cggagaagcc caccacgctg gcacccaggc ggtgcataga
     1741 tgtttcgaaa gagaggcggg tacgggtaga ggcttcgaag aaacagctgg caatgacttt
     1801 gtgcttcaac agctctggtt gcgggtttgc tttcagtttc gccgctgtcg ccagcaccag
     1861 attaaggtca tcgcgactaa ggtcgtttat ggaaatgata tgtttctgat atagcggatt
     1921 agccatcttt tatctcctga cgcctgggca aaaaaaagcc cctcgattga ggggctggga
     1981 atgggtgatc aacgggaaga aaaacggcag gccagcgtct tttttcagac gcggtaagac
     2041 aaaatgtcga acacactgaa ccatacatcc tcccggcaaa ttgtccggca ttatactcat
     2101 cgtcagaagc ggtgcaagca tttgatgcaa tattttgtca gcgcaaacgg tttatttgaa
     2161 ttaaaagtca aggtatatgc atttttattt gatgtgattc tgcaggggaa cggtcgttcg
     2221 gtatcaataa ttgcagacat ttcctgcttt tttctttttc actttcacgc aatcagatat
     2281 gcattttatt ccattcatcc gacttatagg gcggagtgtg aaaacgaacg gctaacacta
     2341 ttgcttactg ctcagggatg cgcgctatca ctttaatttc aaaatcaaag cctgccagcc
     2401 atgtaacacc caccgccgtc cagtttggat aaggtggggc gctaaatatt tcatttttca
     2461 ccgtcatgat gtcttcaaat tggttttctg gatcggtatg gaagctcgta acatcaatga
     2521 tatcgtcaaa agtgcatccc gcagcagcca gggtcgcatg caaattatca aatgccagtc
     2581 tgacttgttg ctgaaaatcg ggttctggtg ttccgtcctc tcgacttcct acttgcccgg
     2641 aaacaaacag caaatcgccg gaacgaatag ccgcagaata acgatgttca gcatacagtg
     2701 aatgtcggcc tgcagggaaa acagcggttc tttctaccat ttggttatcc tcaagattta
     2761 cgacatgaac agaagattta tatttaccag gagccgcttt tagcggacga cgtgagtaaa
     2821 caaaacccag acatcatgga taatggctgg gcttaattga gcgtagtcgg ttatgcgcca
     2881 aacgcgccat caatggtatg catcgcgccg gtaacaaaac tggcttctgg ccctgctaac
     2941 catgcgacca taccagcgac ctcttccggt tgcccatgtc ttttgatagc catcaaacta
     3001 tgcaacatat cgcgcattgg cccgttggcg ggattagcgt cggtatcaat tggccctggc
     3061 tggacgacgt tgatggtgat cccacgcggt ccaaaatcac gggccagccc gcgcgacatg
     3121 ccttgtaggg cagatttact ggcggcataa gcagccatgc ctgcaacagg catacgatcg
     3181 ccattcacgg agccgatgat taagatgcgc ccgccttcgg gcatctgccg ggcggcttca
     3241 acagaggcat ggtaaggagc atgaatattg attttgaaaa ggcgatcaat atcgtcggca
     3301 tttaattcca gggcatcgcc aaagacgcca atacctgcat ttaccaccag gatatccaat
     3361 gcgccgctct tacgaacgac atcaataaca gcgtctctgt cagcactatc tgtgaatact
     3421 gctgttgctc cagtctcttg tgccaggcgt tcagcggcat ctttcgaccc cgcataggtg
     3481 aatcgtacat tggccccatc ggtgacgaaa cgacgtacga tagcggcacc gattccacgg
     3541 ctaccaccga ggatgagaac tgtcttacct gtaaaagcgc ccataaggac tccttgattt
     3601 attatgtaat atgcattaca aaactgtttt aactttctgt caacaagttt tgtaatggtc
     3661 actaaaaaac aatctcgcgt tccaggtcgt cccagacgtt tcgctcctga gcaggcagtc
     3721 tctgcggcaa aagtgctttt tcaccaaaaa ggtttcgatg ctgtcagtgt tgctgaagtt
     3781 actgattatc ttggtattaa ccccccgagc ctctacgcgg cttttggcag taaggctggg
     3841 ttatttagcc gtgtactcaa tgaatatgtc ggtacggaag ctattccgct tgtcgatatt
     3901 cttcgtgatg atcgtccggt aggggagtgc ctggctgagg tattaaaaga agcggcgcgc
     3961 agatatagcc aaaacggcgg ctgcgctggc tgtatggttc ttgaaggcat tcatagtcat
     4021 gatccacaag cgcgtgatat tgccgttcaa tattatcacg ccgcagaaac ggccatttat
     4081 gactatatcg ccaggcggca tccacaaagc gcacaatgtg tgactgattt tatgagtacc
     4141 gtgatgtcag ggctttctgc gaaggcacga gaggggcact ccctcgaaca actctgtgca
     4201 acagctgcac tggcgggggg agcgataaaa actattctca aggagtgatg cgggccttga
     4261 cccgaaaggc gggaacgcgc ctgccgataa gttgtgataa gacaataatt cacgcgttaa
     4321 ggctagcgga attgatcatc ttttcgtata acgatagaaa tgaaacgttg ttttaattaa
     4381 ggagtggaaa agatgatcat cggaaatatt cataatcttc agccgtggct accccaggag
     4441 ttacgccagg cgattgagca tatcaaagca cacgttacgg tagaaacgcc aaagggcaag
     4501 cacgatatcg aaggcaatcg tctgttttat cttatctcgg aagatatgac cgagccgtac
     4561 gaagctcgcc gtgcggagta tcatgcccgc tatctcgaca ttcagattgt gttaaaaggt
     4621 caggaaggca tgaccttcag cacgcaacct gcaggcacgc cggataccga ctggttagct
     4681 gataaagaca tcgcattttt gccggaaggc gttgatgaga aaacagttat ccttaatgaa
     4741 ggtgattttg ttgtgtttta tccgggggaa gtgcataaac cgctgtgcgc agtgggcgcg
     4801 ccagcccagg ttcgcaaagc agtagtgaag atgctgatgg cgtgatgact tttcgccgta
     4861 aataactcca ggtttacggc gagtttgtga aaagagcgtt ttttgatatt tttttgtgag
     4921 tagaatttgt aatgcttaga cgttcttatt aactcaagga gtccgtcatg agcaaaatat
     4981 caggttggaa tttttctcaa aacattacat cagccgacaa ttgtaaacaa aaaaatgaag
     5041 acttagatac ctggtatgtg ggaatgaatg attttgcccg aattgccgga gggcagaata
     5101 gcagaagcaa tattctttct cccagagcat ttttggagtt tttggctaag atatttaccc
     5161 tgggttatgt ggattttagc aaacgctcca acgaagcggg tagaaatatg atggctcata
     5221 ttaagtcctc atcttatatc aaaaataatg atggtagtga gataatgaag tttgttatga
     5281 ataatcctga aggggagcga gcggatttat ctaaggtgga aatagaaatt acacttgcct
     5341 cagcttttaa taatgggatt cgtgaaggac atacagtcat tatatttcca caacctgatg
     5401 gttcgactaa tcgttatgaa gggaagtcct ttgaaagaaa agatgagagt tcattacacc
     5461 tgattactaa caagattctg gcgtgttacc aacgcgaagc taacaaggaa atagctcgtc
     5521 tattaaatat tccgcaggag ttaaataatt ctcaggactt gaataactcg caggtgagtt
     5581 gtaaagattc agttgattct acgattacgg atttattaga aaaaccattg aataatgcat
     5641 tattagcaat aaggaaagaa catctgctat taatgcctta tgtatgtaat gaatcgattt
     5701 catacttgct tggtgaaaaa gggatactga aagaaataga tgaccttaac gcagtaaata
     5761 attatcttct taataataag aaagctactg acaatgaaat taatgacatc aaagttaacc
     5821 tgtctcatat tcttattgct tccttagatg atgcaaaagt taaccttac
//
LOCUS       DAZGXZ010000232         5865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000232 DAZGXZ010000000
VERSION     DAZGXZ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.233"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(324..1283)
                     /gene="nrdF"
                     /locus_tag="ACHKJF_005115"
     CDS             complement(324..1283)
                     /gene="nrdF"
                     /locus_tag="ACHKJF_005115"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0952316.1"
                     /translation="MKLSRISTINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(1293..3437)
                     /gene="nrdE"
                     /locus_tag="ACHKJF_005116"
     CDS             complement(1293..3437)
                     /gene="nrdE"
                     /locus_tag="ACHKJF_005116"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0952317.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(3410..3820)
                     /gene="nrdI"
                     /locus_tag="ACHKJF_005117"
     CDS             complement(3410..3820)
                     /gene="nrdI"
                     /locus_tag="ACHKJF_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP0952318.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(3817..4062)
                     /gene="nrdH"
                     /locus_tag="ACHKJF_005118"
     CDS             complement(3817..4062)
                     /gene="nrdH"
                     /locus_tag="ACHKJF_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP0952319.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(4310..4639)
                     /locus_tag="ACHKJF_005119"
     CDS             complement(4310..4639)
                     /locus_tag="ACHKJF_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP0952320.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            4791..5135
                     /locus_tag="ACHKJF_005120"
     CDS             4791..5135
                     /locus_tag="ACHKJF_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP0952321.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(5172..5621)
                     /gene="alaE"
                     /locus_tag="ACHKJF_005121"
     CDS             complement(5172..5621)
                     /gene="alaE"
                     /locus_tag="ACHKJF_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP0952322.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         1512 a         1430 c         1440 g         1483 t
ORIGIN      
        1 cgatatatta cccaatggaa atagtcactt ttctctaccc taacatactg agaatctgag
       61 gcaacccctg agggcaaatg tggcatgaat attgatgcga gcaaatcgcc ggaatttccc
      121 gtgatataag ggctgagagc aaatcgagaa aagttgatat tttttgtcat agatagcgaa
      181 aaaacgcctg aatattgtag tttcaggaga ataatgcaac agagtatcag tgtagatcac
      241 tacagaatat ttgcgtgata aaaagctatt tggcgggaat tatttccctg ctgcgggtag
      301 tgatattttt gaaaataaca ccctcagaaa ttccagtctt catcttctgt ttcaaccgct
      361 ttccccatca cataagagga gcctgaaccg gaaaagaaat cgtgattttc atcggcattc
      421 ggcgaaagcg cggcgaggat tgccggattc acttccgcca tttctgcggg aaataacggt
      481 tcgtagccca gattcatcaa agccttattg gcgttgtaac agagaaacgc tttcacatcg
      541 tcagcccacg gggtttcggc gtacagctca tcggtgtatt gcaactcgtt gtcgtagagt
      601 tccagcaaca aatcgaaggc gaaactcttc aactcttcac gttgtcccag agatatcttt
      661 tccatgtttt tctgatattt atagcctatg tagtaaccgt ggactgcttc atcgcggata
      721 atcagacgga tcagatctgc ggtattggtc agctttccgc ggctggaaaa atacatcggc
      781 agccagaaac cggaatagaa caaaaaagat tcaagaaaca cactggcgat tttctttttc
      841 agcggatcat caccgcgata atgttgctga ataatctgag cttttcgctg caacggtgcg
      901 ttttcttcac tccaggcgta ggcggcatcg acatctttgg tctgacacag cgtcgagaaa
      961 atcgaactgt aagagcgggc atgaaccgct tccataaagc tgatattcga taataccgct
     1021 tcttcatgag gcgtgagtgc atcgggcatc agcgaaggcg cgccgataac attttgcagc
     1081 gtgtcgagca gcgtcaggcc agtaaaaacg cgcatcgtca gttgttgttc tacgacagtt
     1141 aatgtctgcc aggcaggaat atcgttcgac agcggcacct tttctggtag ccagaaattg
     1201 ctggtcaggc gattccacac ctccagatct ttatcgtcag atatcttgtt ccagttgatg
     1261 gtgctgatac gtgagagttt catagatatt ccttaaagtg cacaggagac gcagccttca
     1321 atttcagtgc cttccagcgc catctgacgc aggcgaatgt aatagagcgt tttgatacct
     1381 ttgcgccagg cgtaaatctg cgctttgttg atatcgcgag tggtggcggt atcggggaaa
     1441 aacagcgtca gcgacaaacc ctgatcgaca tggcgagtcg cttccgcgta ggtgtcgatg
     1501 atcttttctg cgccgatttc gtaagcatcc tgatacagcg ccagattctc gttagtcata
     1561 aacggggcag ggtagtaaac gcgtcctgtt ttgccctctt tgcgtatctc tactttcgcc
     1621 acaatcggat gaatactcga cgtagcatgg ttgatataag agatagaacc ggttggcggc
     1681 accgcctgaa gattctggtt ataaatgccg tagcgcatca cgtcgtcgcg cagttgcgcc
     1741 cacatctcac gggtaggtaa cgtaataccg ctgcgggcaa acagttcgcc aactttcgcc
     1801 gttttcggct gccagttacc ttgcagatat tggctgaaat attcaccact ggcatagcgt
     1861 gactgtttga acccggcgaa ggtttcaccg cgttcgcgcg ccagcaacat cgaggtacgc
     1921 agtgcgtgcc aggtgatggt atagaaatag agattggtga aatccagggc ttccggcgaa
     1981 ccataagcga tgccttccct cgccagataa ccatgtaaat tcatctgccc cagtccgatg
     2041 gcgtgcgagg cggcatttcc ggcttcgatg gacggcacgc tgcggatatg actcatatct
     2101 gacaccgctg ttaaaccacg tacggcagtc tctaccgtgc gggcaaagtc gggggaatcc
     2161 atggtgtgag caatattcaa cgaacctaaa ttgcaggaga tatcatggcc tgtgcgggca
     2221 tagtcgagat tctcgtcata ctctgaggca cagttgacct gcaaaatctc tgagcaaaga
     2281 ttgctcatat ttatgcgccc ggcgataggg ttagcacggt ttactgtgtc ttcatacatg
     2341 atgtagggat agccggactc aaactggatt tctgccagtc gctggaagaa atcacgggcg
     2401 ttgaggtatt ttttgcggat gcgttcatcg gcaaccagtt cgtcatagtg ttcgctaatg
     2461 gcgatatcgg caaacggctt gccataaact cgctcaacgt catagggcga aaacagcgcc
     2521 atctgcgcat tctcttttgc cagatggaaa gtgatatccg ggatcaccac gccaagcgac
     2581 agtgttttaa tgcggatttt ttcgtcggca ttttcccgtt tcgtgtcgag aaaacgcaga
     2641 atatcgggat gatgagcatg taaatagact gcacctgccc cctgacgagc gccgagttgg
     2701 ttggcatagg aaaatgcgtc ttccaacatt ttcatcaccg gaattacgcc agaagattga
     2761 ttttcaatac gtttaattgg cgcgcccgct tcccgcagat tcgacagcaa aaatgctacg
     2821 ccgccgccgc gtttcgacag ctgcagtgcg gaatttaccg cccgaccaat cgactccata
     2881 ttgtcttcaa tacgcagcaa aaaacaggaa accagttcgc cgcgctgctg cttaccgcag
     2941 ttgaggaatg ttggcgtggc tggctgaaag cgtcctgaca gcatttcatc ggtcagttgc
     3001 aacgccagcg tctcatcgcc ttgtgccagt gtcagcgcca ccatcgttac tcgatcggca
     3061 aaatcttcca gataacgttt accgtcgaat gtcttcaacg tatagctggt gtaaaacttc
     3121 catgccccga ggaatgtctg gaaacgaaaa ccgctggtgt gtgcgtgggc aaacagcgta
     3181 atgacaaagt cgcgagagta gcgattaaga acgctttcat catagtaacc ttcgttgacc
     3241 agccagttca ggcgctgctg ctggctactg aaggtcacac tgttcggacg cacatgcgtc
     3301 gcaataaagg cgtcaacggc ctggcggtct ttatcgaact gaatgcgacc tgcgctatcg
     3361 tagaggttaa gcatcgcatt cagcgcgtgg taatccatcg tttcctgcgt caggcattct
     3421 gcggttgtcg ttgccaaaat tcggttactc ctttacgaac gttttcgata tcgctttgcg
     3481 tacccatgag ttcaaaacgg tacagccacg gcacgccgca tttccgggca atcacatctc
     3541 cggcgcggcc atacgcctca ccaaagttgc gattaccaga agcaataacg ccgcgaagca
     3601 acgcccggtt gtgctcgtcg tttaaaaagc gaattacctg tcgtggcacc gcgccagccg
     3661 taccgccgcc gccgtaagag ggcacgatca ggatgtaagg ctcgtctacc tgaatccgtt
     3721 cccgctcatt gagcgggatg cgcaccgcgg gcagacctaa acgttcgata aaacgctgcg
     3781 tgttttcgga gctgctggag aagtagacga gctggctcat gcactggccg cgtgtggcgc
     3841 tggatgcaga cggttaatca tgtccggacg gaaaccagac cagctaagat cgccagcaat
     3901 cactaccggc aactgacgaa agccctgagc acgcaacgct tctgccgctt caggaacgcg
     3961 atcgacatta atcatttcaa aatcaaagcc ccgattttcc atcgcccgtt tggtggcgtg
     4021 gcactgaacg caatcgttac gagtgtaaat agtaatgcgc atgattcgta tttccgttta
     4081 aaatgaagat acggcgcgat gatacgcgtc gggttgtctt tctgttgata cagagatact
     4141 agatgtagtt gaaaaaagat tcaaccacac aatatatagc aaatagtggt cgaaattacc
     4201 ctggatatga gcgtgacggg gtagggggat ttttgtgatt caccaggcaa aaagaaaccc
     4261 cgaagacagg cttcggggtt aaagacgcgt atttattatc atttttgcac tacgatttgc
     4321 gcatgcttaa cagtgcgccg ataaaaatac ctaccgcagc tgctgtaccc acgctacacc
     4381 agggtctttc acgaacaaaa gaatccgcgc agccaacggc atcgcgtgcg gcttgctgga
     4441 cgcgagtacg accatgcatt cgcgccctgg tttctttcag caatgcctga gctttactgc
     4501 gtgcggcttc agcttcccct ttggcgtcgc tgccccagga cttcaataca gattcgaggc
     4561 tgtcagctaa ttgattgaca tcattctgga tatcctgcac gccatcatca acgtcgttgc
     4621 ggttcggtcg gttaaacata tgctccccca ttagaattcg atgtaatttt cagtgtagac
     4681 cataattttc ttaactgagg ttcatcgcgg ctttatggac ttttctgaaa gcgttgtgaa
     4741 tgcgcaatca ggtaaggtag gcccgcggtt gataataacg aggatcaaat atgtatttac
     4801 gaccagacga ggtggcgcgc gtacttgaaa aagtcggttt tactgtcgat gtggtaaccc
     4861 agaaagcgta tggttaccgc cgtggggaaa attatgtcta tgttaatcgc gaagcgcgaa
     4921 tgggacgtac cgctctggta attcatccaa cattaaaaga acgcagttcg acactggctg
     4981 aacccgcttc cgatattaaa acttgcgatc attaccagca atttccgctc tatttagcag
     5041 gcgagcgaca cgagcattac ggtatcccgc atggctttag ttcgcgtgtt gcgcttgaac
     5101 gttatttgaa tggtttattt ggcgaagcca gttaaagacg cgactggcga tgccagtcgc
     5161 gaaaagaaga gtcaggcttt tacctgctgg taacggctga ctttaaacag tcggcggcaa
     5221 taatcgagga agtagccata aaccgccccc atcaacatcg aaacaacgat gtttgaactg
     5281 accgccgcca taatctgatg ccagtctgcg cccaccacta acaagatcgc cacatacacc
     5341 ggtgactgga acgtcacata agccaggata tccgccagat tttttatcca gcccgacggg
     5401 ctaactttgc gtgccgcgcg cataaacaga tcacggtaca taccgtatgg ccatgcaatt
     5461 aagatgttca ccggaatcgc taccaatctg gaataaaaag actgttcgaa gctcattccg
     5521 gagaggaaaa cttcaataca catgttcacg acagaacagt aaacaaccat cgcgaacgta
     5581 tctgcaactg catgacgcaa gcgtgactgc ggtgagaaca tgctaatgct ccttaataaa
     5641 agcgtaatgg gatgttaatg gagatgaaaa cagtggatct cattgccatc tgcattttcg
     5701 catagcgtat atcgctggct atgactaatc aattagtgaa aaatttttat ttagtcagtt
     5761 tttgtagata aaatattccg taacggctcg ttttttgtac attgtggatt tttacatgcc
     5821 gatatgattt attttcaatt aaatcaatta gatatgacct ttcct
//
LOCUS       DAZGXZ010000233         5832 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000233 DAZGXZ010000000
VERSION     DAZGXZ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5832
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.234"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..130
                     /locus_tag="ACHKJF_005122"
     CDS             <1..130
                     /locus_tag="ACHKJF_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0952323.1"
                     /translation="REVVKACNASARVADNLPQILADYLKETTGNVPEKLLQKKGE"
     gene            386..667
                     /locus_tag="ACHKJF_005123"
     CDS             386..667
                     /locus_tag="ACHKJF_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952324.1"
                     /translation="MIEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVAEVMR
                     RDGIELNAVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            909..1067
                     /locus_tag="ACHKJF_005124"
     CDS             909..1067
                     /locus_tag="ACHKJF_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952325.1"
                     /translation="MVNGGVADLQLDHETTETTRSLGSKSNTQILGLTRDTQIWGIGG
                     NHNDFRYG"
     gene            complement(1387..1530)
                     /locus_tag="ACHKJF_005125"
                     /pseudo
     CDS             complement(1387..1530)
                     /locus_tag="ACHKJF_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            1942..2307
                     /gene="mdtJ"
                     /locus_tag="ACHKJF_005126"
     CDS             1942..2307
                     /gene="mdtJ"
                     /locus_tag="ACHKJF_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP0952326.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2294..2623
                     /gene="mdtI"
                     /locus_tag="ACHKJF_005127"
     CDS             2294..2623
                     /gene="mdtI"
                     /locus_tag="ACHKJF_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP0952327.1"
                     /translation="MAQFEWIHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(2662..3483)
                     /gene="ydgD"
                     /locus_tag="ACHKJF_005128"
     CDS             complement(2662..3483)
                     /gene="ydgD"
                     /locus_tag="ACHKJF_005128"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP0952328.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(3583..3666)
                     /locus_tag="ACHKJF_005129"
     CDS             complement(3583..3666)
                     /locus_tag="ACHKJF_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952329.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(3759..4067)
                     /gene="asr"
                     /locus_tag="ACHKJF_005130"
     CDS             complement(3759..4067)
                     /gene="asr"
                     /locus_tag="ACHKJF_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP0952330.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            4338..4442
                     /locus_tag="ACHKJF_005131"
     CDS             4338..4442
                     /locus_tag="ACHKJF_005131"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP0952331.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(4491..5744)
                     /gene="ynfM"
                     /locus_tag="ACHKJF_005132"
     CDS             complement(4491..5744)
                     /gene="ynfM"
                     /locus_tag="ACHKJF_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0952332.1"
                     /translation="MSRTTTVDGASASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
BASE COUNT         1572 a         1350 c         1440 g         1470 t
ORIGIN      
        1 aagggaagtg gttaaagcct gtaatgccag tgccagagtt gccgacaacc tcccacagat
       61 actggctgat tacttgaaag aaacaaccgg aaacgtaccg gaaaagttgc tacagaagaa
      121 aggcgagtaa cagacgcgca attattgaac aaaactgaga aacgacatga aagcgtcata
      181 aatcgccatt ttagatgatt accgtgtcgt ttctttttat tgtgtatcta tttaaaaaca
      241 aatagttatg ttcgagaagt accgacatgc tttttccaga aaaattttca taaacagata
      301 aaaaccgcga ggtcgccgcc ccgtaacggg ccataattcc aggaaggacc cgacgacacc
      361 agactttcag aacgatgggg gcacaatgat agaagccgaa atactgggat taatccgccg
      421 cgttgccgga atcagccagc aggttgacga acaggccatg cagccggaca gcgtgaccgc
      481 tgaaaattat gcgcgtgttg ttgcggaggt gatgcgccgt gatggtatag aacttaatgc
      541 cgtggatatg cgcaacatac gaaccagagt tcttgagttg ctggcctacc gtcgccgcgt
      601 ggagatgtat cgggagaagg agaaaataac gtaccactgg aagaagccgg agcggttgcg
      661 gcggtaactg gttgatattc ccgacggccc aaaaatgggt tggctacacc cctcacaact
      721 acgacatttt cgcagtttaa ttaatggtca gcttaatcgc taacccgctg atattttcgg
      781 aaacctcaat ttgaggaagt cggcgcggta actcgctgaa ctttaagcaa agcgcaaaat
      841 tgcgcttgct gaataatcat tatgattacg cagatgatta aggaatgacc gaaggcggaa
      901 attcgcctgt ggttaatggg ggagttgcag atctgcaact cgaccatgaa actacggaaa
      961 ctacccgtag tttgggtagt aagagtaaca cccagatttt ggggcttact cgtgataccc
     1021 aaatttgggg tatcggtggc aaccataacg actttcgtta cggttgatgc ttttacccca
     1081 ttgggggaaa ggtattacga taatcataac acctaccgaa caggtaaagc ccaccagcct
     1141 gattaacgtt taaccggaaa aaaattccgg taggtgggga tccccatatc gacattaacg
     1201 cccctcatga attggtgcgc ttccccctgg aaagatgatc cgcctgtata tttcttgtgt
     1261 ctatttgttc cacgttgttt catacggtgc accgaacggt gtagtcactg gtgtagtcat
     1321 tttgcgattt ttggcacttt ttgtggtgca aattatttaa ttaaatcaat gcgttaagtg
     1381 aaataagtta tatataacag caataaataa tgcgagaata aacggcacga tgatctcggc
     1441 ggcaaaacgg ataccgcaga gaataatgac cagcattccc aacataatga cgatttttag
     1501 gccattgagc gtgatgatcg gctttgccat gcttgtcccc gttttttctt cctttgctaa
     1561 taataatgtc tttttcgctc cgtcatcata acgaaaatca aaccgctcag gtaaagaagt
     1621 gaaaatattt tgagttaatt cttaagctat gatacaaatc aggcgtgttc aactaccgag
     1681 gacaattatc atccgcgatg acgagaagca acactgcgga taattgtaat attatggaca
     1741 atttgttcag gacgttattt tcaacattta cccatcttcg tacctcatcc actatcttgc
     1801 tcgtaggtga gcagcactgg cgcaacgcgc tttagtcctt tcttctgcct aagcttgccc
     1861 tatgcgcggg gcttgaccac tttactttgg tttcgctgaa ttaagcgaaa attaaaataa
     1921 ttctcttgca ggagaaggac aatgtatatt tattggattt tattaggcct ggctattgct
     1981 acagaaatta ccggtacgct gtcaatgaaa tgggcgagcg tcagtgaggg aaatggcggc
     2041 tttattttaa tgctggtgat gatttctctg tcgtatatat ttctctcttt cgccgttaaa
     2101 aagatcgcct taggcgtagc ttatgcgctg tgggaaggta tcggtatttt atttattacc
     2161 ttgtttagcg ttttgttatt cgacgaaagt ttatcgctga tgaaaattgc cgggttaacc
     2221 accctggtcg ccgggattgt gttgatcaaa tcaggtaccc gtaaagcgcg taaacctgaa
     2281 ctggaggtga accatggcgc agtttgaatg gattcacgcc gcctggctgg cattggcaat
     2341 cgtgctggaa atcgttgcta acgtcttttt gaaattttct gacggctttc gtcgcaaaat
     2401 atttggcttg ctctccctgg cggcggtgct ggctgccttt agtgcgcttt ctcaggccgt
     2461 aaaagggatc gacttgtctg tcgcttatgc attgtggggc gggtttggta ttgccgccac
     2521 gttagccgca ggttggatct tgtttggtca acggttaaat cgtaaaggct ggattggcct
     2581 ggtcttgctg ttggctggaa tgatcatggt gaaacttgcc tgatgaagac gctgcccgcg
     2641 ctgtcgggca gcgtttgaac attatttttg cgacagttga tccagcttgt cgcgaaaacc
     2701 ggtaacagaa atggcccggt tatcggcgcg ccagcgatct ttcgcggcag gagccgaact
     2761 ttgcacccca attaattgcc agccgtcatc ggtatgcaac atcagaggcg aaccgctgtc
     2821 acccggcaag gtatcgcact gatgtgacat caccgacgtt tgcgcccagc cagtcacttc
     2881 acagttttga tgactgtata acgtatcgag atgatcttca ggatatcccg cctgagtcac
     2941 tttacgacca gccgatttta atgcggcagt aagcgcggct ttatctccct caaataacgg
     3001 caacggtgta atgccagaag ggggattacg cagcacaatc aagccgaagt cccatggtgc
     3061 agctgcggga ggaacaatcc aaccatcccc atctgctttt aaccgctttc ccagtgtcgg
     3121 atcaacgcgg ccttctatgt cgtggatctc atagcgccaa agacctttat ttgacacaaa
     3181 acgcagcgcc actgctttat cggctttacc ctttggaggt gtcaataaac agtgtcctgc
     3241 cgttaatgcc agattgggtg caatcagcgt cgccgtacat aaattgccgc tggccgtttc
     3301 cagttgccca accgcatccc acggtgattg ggtcgtgtca ttcactggca cacgatcatc
     3361 atgaccaaaa aacagggtgc tgacctcatc gtttgccgat ttggcaacgt ctggtttatc
     3421 tgcaaacaca aaagcagacg tcaaactaat tgcacccaac actacagcaa tggttgtacg
     3481 catatcacac tctggtgggt aattatgatt attaaaagca aaccctcata aatactatag
     3541 acgggacggg atgaaagtgg gagtaaaatc agataactac agtcaggaaa gataaaaacg
     3601 ggcggcaatc agtgcgcata aaataaggat gataagaatg aactcaaagc gataacggcc
     3661 caccatacta ccctccgcta aaggcggtgc taaacgttac ccgcttagca ccgtaatttt
     3721 caggcgcgag ggggcgcgcc agcattactg ttgaaaactt acgctgcggg ttgtgcagca
     3781 ggttttgccg gttgctgatg gctgtgtttc ccggcgtgtt tcttcgctgc ctgcgctttt
     3841 tgttcagggg ctttctgttc agctttcgta tttttatgat gctttttagc cgcctgcgct
     3901 ttctgggcag gtgctgcttt atgctgtttt ttatgatgtg tcgttttcgc cggcgctgct
     3961 ttggtggtcg tcgcagtcgg agcaggtgtg gtcgcagtct ctgcagcaaa ggcggcagaa
     4021 gacagaccca tagcagcggc aacaaccaga gctaatactt ttttcattgt cataccctca
     4081 atttgttttt tcatttaacc ccactgcggg gccgttgaaa taactatatc cctgggaatg
     4141 gcagacttcc gtgagtggtt ggtttcagcg tgtaacgata tgtacaaacg ctgaataaat
     4201 tacagcgttg ataataacgt attgtgactt aagggaaatt tagctaccaa taatagtagt
     4261 cttgatcggg ttaactattt tactacttac aaagcggtag aataactgcg catcagtaat
     4321 aaatgacaca cagcaaaatg aatccgttta tttgggtact tataatcctg atgacgctag
     4381 acgcgctgcg ggaattggct ggcgcttcgt ctattttagg atggctatta acgttggttt
     4441 gagcaagcaa taagtccgga cgtgtagtta ccgcagtccg ggcttatttt tcaggcgtgc
     4501 agacgacgat gcaaacgcgt cccgaccagc agagcaatga ccagcatcag agcaataaat
     4561 gcgccgacgc cgttccagcc atagttatgc cagaaaacac cacccagcgt cccggcaata
     4621 ctcgacccca gatagtaact gaacagatac agcgaggagg cctggccttt agcgcgtttt
     4681 gcgcgggggc caatccagct gctggcaact gagtgggcag cgaagaatcc tgctgagaag
     4741 agtaacattc cggcaaagat cagccacagc gagctgaata aggtcatcag taaaccaaac
     4801 agcataaccc ccgtcgaaaa caacatcacc ggaccgcgcc cgtagcgggt ggtcatggtt
     4861 ccggctttgg gtgagctcca tgtaccggtc aaataagcca gcgataataa gccaaccacg
     4921 gcctgactga catgccaggg ggaaagcatc aaccgatagc cgatgtaatt aaacagcgtg
     4981 acgaacgacc ccatcagcaa aaagccttct gcgaacaata acggtaatcc ccggtcacgc
     5041 cagtgcagac gaaagttgat aaataacgtc ttagggcgca gcgaagtcgg gcgaaaatgg
     5101 cgtgattccg gaaggatttt ccagaacatc aacgccgagg ccagcgcgaa acaaccgatt
     5161 gccgccagag caattcgcca gttgaaaaag tccgtgaaga caccactaat taagcgtccg
     5221 ctcatgccgc caattgagtt gccgctgata tacaacccca tcgaaaaggc cacgaaactg
     5281 gggtggattt cctcgctaag ataagtcatg ccaacagctg ccacgccact taacgaaagc
     5341 ccaatcaagg cgcgcataat caaaatgccg tgccagctgg tcatcattgt cgaaagtaac
     5401 gtacaaatgg aggccaacag tagcgccgtg accatcactg gtttgcgacc aatggcatcg
     5461 gatagggggc cagtaaacag caaaccaata gccaacatcg ccgtggaaat ggacagtgaa
     5521 atactactgt tcgcgggggt taagccaaac tcctgcgaaa gcaccggaag gataggctgc
     5581 acacaataga gaagtgcaaa ggttgccagt ccggcagaga acagcgccag ggtgacgcgc
     5641 ataaattgcg gcgtaccgcg tttaataaat tgatttggct gagaaatgct ttgcttgtca
     5701 gtgtcgcttg ccgaagcgcc atcaacagtt gtagtacggc tcacttgaaa tccttgctaa
     5761 atatgcctgt ggatcaggct tatacatagg gtaggaaaat cgaattgttc tgtctaatat
     5821 attaataatc tc
//
LOCUS       DAZGXZ010000234         5756 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000234 DAZGXZ010000000
VERSION     DAZGXZ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.235"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1038
                     /locus_tag="ACHKJF_005133"
     CDS             <1..1038
                     /locus_tag="ACHKJF_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0952333.1"
                     /translation="ANATTLKPFSVALGAGSVADRGAGIEAYMPETKRWNPGDVVKSR
                     MSDKQLNRLMEIDKLISAYNSQETLSAEETKQLEEVKKERADMFATYIATHDAVSVGV
                     GATTTGRHAVTRQITSVAAGSVDTDAVNVAQLRDLQTVTEKADAALDKRITNNTSAIT
                     KNLQVVNSNKEEISNINKTTKILDERVTANTQTAEEHKLLISENSKQISTNTLGIREN
                     TQRVQDEGQARIRGDRETLRAATGYTDMRVNGLERNTNKRFAQLKEDIDKNRKRADAG
                     IAGAMAMTGIPSVPGKKVSFGMAMAGYRSEGAVAAGFHFNTSENSAVKVNAAWDTENG
                     AGVSAGMALGW"
     gene            complement(1468..2043)
                     /gene="dolP"
                     /locus_tag="ACHKJF_005134"
     CDS             complement(1468..2043)
                     /gene="dolP"
                     /locus_tag="ACHKJF_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001238641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0952334.1"
                     /translation="MRLLYSAVVLISALLLQGCVAATVVGTAAVGTKAATDPRSVGTQ
                     VDDEVLELRVSSALSKDEQIRKEARISEIAYQGRVLLIGQSPDRELSARARQIALGVD
                     GVQEVYNEIRQGTPVSPVIMAGDAWLTTKIRSQLLTSARVKSSSVKVVTENSEVFLLG
                     LVTEQEGRAAADIASRVSGVKRVTTAFSCIR"
     gene            complement(2389..2616)
                     /locus_tag="ACHKJF_005135"
     CDS             complement(2389..2616)
                     /locus_tag="ACHKJF_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HFP0952335.1"
                     /translation="MFPEYRELISALKGNHKRFDSLFEKHNKLDHEIKRIEDEFGSAY
                     STKVVRLKLEKLEIKREIQKILEDESEKGER"
     gene            complement(3288..4379)
                     /locus_tag="ACHKJF_005136"
                     /pseudo
     CDS             complement(3288..4379)
                     /locus_tag="ACHKJF_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            complement(4642..4830)
                     /locus_tag="ACHKJF_005137"
     CDS             complement(4642..4830)
                     /locus_tag="ACHKJF_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0952336.1"
                     /translation="MRLLIAFLLPWLTFFTIGRPLTGIFCLVLQITLLGWIPATIWAV
                     YALSQYKTEQKIREATGK"
     gene            complement(4905..5420)
                     /locus_tag="ACHKJF_005138"
     CDS             complement(4905..5420)
                     /locus_tag="ACHKJF_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001210963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0952337.1"
                     /translation="MRFICDNSYFLEGVKEYINPGKFIISGMQTDNVVIISSSRISEI
                     VGFLYKNNISDMNTLYFSSPEALRFLWGVVDSPVYDIRHLTERCSVNDISHLYRAFMS
                     VEKLTLSGRQVNVLMMLLYGSNGTEGARYLGMSEKTFSTHKQNLIKRLRVHNEVELLY
                     KGMLLVRKGRL"
     gene            complement(5544..>5756)
                     /locus_tag="ACHKJF_005139"
     CDS             complement(5544..>5756)
                     /locus_tag="ACHKJF_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0952338.1"
                     /translation="AWLFGHTARPLTRTEMVVVFGVIFHEYGCTFLADQLGISMKTVC
                     AHLYNAMEKSGLRGVSVKYLCSTADR"
BASE COUNT         1700 a         1370 c         1187 g         1499 t
ORIGIN      
        1 gcaaacgcaa caacattgaa accattctct gtcgctctgg gcgcaggaag tgttgctgat
       61 cgtggcgctg gtattgaagc atatatgcca gagacaaagc gctggaatcc aggggatgta
      121 gtaaaatcca ggatgtctga caagcagctt aacaggctta tggaaatcga taagctaata
      181 tccgcttata attcacaaga gacgctgtct gctgaagaga ctaaacaact ggaggaagtg
      241 aaaaaagagc gggctgatat gtttgcgaca tatatagcaa cgcacgatgc tgtatctgta
      301 ggtgttggtg caacgacaac aggacgacat gctgttacaa gacagataac tagcgttgca
      361 gcaggttctg tcgatacgga tgctgttaac gttgcacagc tcagggattt gcagactgtg
      421 acagaaaagg cagatgcagc gcttgataaa cgcataacaa acaacaccag tgcgataact
      481 aaaaatctgc aggtggttaa cagtaataaa gaggaaatca gtaacattaa taaaacgaca
      541 aaaattctgg atgaaagagt gacagccaat acacagacag cagaagaaca taagctgctt
      601 atttcggaga acagcaaaca aatcagtacc aacactcttg gtatcagaga gaatacacaa
      661 cgggtccagg atgaaggaca ggctcgaatc cggggagaca gggaaacact gcgtgcagcg
      721 accggttata ccgacatgcg ggtaaacggg ctggagcgta acacgaataa acgttttgca
      781 cagcttaaag aggatataga caaaaacaga aaacgtgcag atgcaggtat tgcaggtgcg
      841 atggcaatga cggggatacc gtccgtcccc gggaaaaagg tctcatttgg tatggccatg
      901 gccggatacc ggagtgaagg tgctgttgct gccggcttcc attttaatac aagtgaaaac
      961 agtgctgtta aagtgaatgc agcctgggac actgaaaatg gagctggcgt ttcagccggg
     1021 atggctctgg gatggtaatg cttccgggcc agaagccgtt cgcagatatt gttatccagg
     1081 ggacactgca ccaccattca ggaagcgatt cagtttatcg catcggctca ggatgtaatt
     1141 aatacggaca atctggctcc tgcaccggag ccagacgtac acgtgtcaac gccacgatct
     1201 ttgacctgta tggataaaag ctcagattat ttaccagcag cgacatccgg agagcaccgg
     1261 gattttatct gtgagtgaac atacccgggc aaaaaaatcc cccgaattca ggggggtgtc
     1321 aaagaggaat tatgtatatt ggttattata atgagcacgt ctccggtatt ccgcgacgaa
     1381 tgttcacgtg gtggcatgaa cggcccggaa acgtgctctt ctgattttta acgtaaattt
     1441 gcactaaagg caactcagat acggccttca ccgtatacag gagaatgccg tcgtgacccg
     1501 cttcacgcca ctcaccctac tggcaatatc tgctgccgct cgcccttcct gctctgtaac
     1561 caggcccagc aaaaagactt cgctgttttc tgtcaccacc ttgacactgg atgatttgac
     1621 acgggcactt gtgagtaact gagaacgaat ttttgtcgtg agccaggcat cacctgccat
     1681 tatgaccgga ctgacgggag tcccctgccg gatttcatta tagacctcct gaacaccatc
     1741 gacaccgagt gctatctgac gggcacgggc agataattca cggtccggag actgaccgat
     1801 cagcagcacg cgtccctgat atgcaatctc actgatgcgt gcttcttttc tgatttgttc
     1861 atcttttgaa agtgcactgc tgaccctcag ctccagcact tcatcatcca cctgcgttcc
     1921 gacactgcgt gggtcggttg ctgcctttgt cccgacagcg gctgtaccca ccacagttgc
     1981 agcaacgcat ccctgtaaca ataaggctga aatcaggaca acagcagaat ataataaacg
     2041 catatctccc ccggaattta tcgtacaaaa agtttacctt cagtgaaaca aaacaagcta
     2101 cggactgtta ttctcttccg gcacagccgg aaacagcgga tacgttacgt gacatatctg
     2161 tatctgccag acggaaatgt ttatgacgga tatggatgta ctgaaattta atattgttca
     2221 tgtttttgca cgaaaacgtt gatagcgtag taaagattct gttttttaac tacctggttt
     2281 gctgagcagg atctcctttt cgttatacac aggctaaaag gcaccgtggc cccccggtgc
     2341 ctttttcata aaacagagaa aacacggctt tgaatatacc gtttatgatt accgttcgcc
     2401 cttttctgac tcatcttcaa ggattttctg gatttctctt ttgatctcaa gtttttcaag
     2461 cttcagcctg accactttcg tcgaatacgc tgaaccaaat tcatcttcaa ttctttttat
     2521 ttcgtgatca agtttattat gcttttcaaa taaagaatca aagcgtttat gatttccttt
     2581 caatgctgaa atcagttctc tgtactccgg gaacattgtt tttatctcct ttcatcaaca
     2641 ctgtaacaat acatataaac tttaaattta ttaaagattc tcaccatcat tcgcagtgaa
     2701 taatgccgca tctcatattc atatcctgca gagaaaaccc ctccgaagag gggcgagtgt
     2761 agcgttaatg gagtgttatg tagcatggtg ccgggtgcct cccggtgaac agactgttcg
     2821 tgataccagt cagcaacata ggaaattaat ggtatcaccc caccgcacag ggggattcac
     2881 catgcctgcc ttttttagca aattcatcag aaaccggaca acatgcttat ttatgaaatg
     2941 aggtgctttt cacatttata taaaatcatt ataacgataa tcctaaaaat caatgtaatt
     3001 atcatcttca gtaactttct gtattacacc atacggaaat tgatttttga cggtaaagat
     3061 acaaggggaa gtatgtctgt aaggagtacc ctgaataatg gacaggcatg catcgtggct
     3121 gttcccctga aattatcagt acaaccagtc agctcataat ccctgtctct ttttccgggg
     3181 agccctcagg gggactttcc cttatccccc cagtttctgg agagacgtaa aaaagaatta
     3241 atcatatgtc aggtatctga atatgtcccg taattaatat atttacgtca ggattttttc
     3301 tgcccgccag aagtatgaac atcttccgcc gttgagtctg tcatatccat ttttttctgt
     3361 gccagtactt catatgattt ttctttttcc agactctcct gctgcatccc gtgagccgga
     3421 gcatatacaa actcagccat cactggaaaa tgatctgaac ccatatccgg taaacggcgt
     3481 aaacttttaa gcagaaaatg ctggctgtga aatacatggt caagcggcca tcgcaggaaa
     3541 aaataatggc tgtgaaacgt gctaaaaaaa ccacgtcccc ggcgtggatc cagcagtcca
     3601 cttattttca tgaataaacg agtggtcgca gaccacgcaa catcattcag atcccccgct
     3661 aatattacag gtaattcaga atctgcaaca cttttcccga ccattaaaag ttctgagtca
     3721 cgatcagttg actcatcact ttcagtcggg ctcggtggaa caggatgaag acaatgtaat
     3781 cttattctct gaccttccgg catatgaact atcatatgca tggagggaat ttccttgtcc
     3841 actaaatact gtatttctgc atcacttaaa atcatccttg aatatacatg catgccatat
     3901 aaattttctt tcgggcattt cagggagtat ggaaacactt catccagagg tgatagtttc
     3961 tgttcccacc ataagtttgt ttcaagaaga acaagtatat caggtttttc tttatttata
     4021 agttcaagca aaggcatata ttttttattg gtcataagga cattgaatac cagtatactt
     4081 aaacccggaa atccatcaga gtcctttatt tactttacct gcttcttgcc aattactgta
     4141 taaggaatta tccataccaa ctggtaagcg acacaaatta aacttattat cacaacaaac
     4201 aactctgcaa atgagtcaag aaaaacaaca gacagaaaaa cattcaaagt acacagcaaa
     4261 agtatctgta gtcggggaaa atcccatacc ctgacaaccc atgaagtatt accggaaaca
     4321 gggataaaag ttatgactgc cagaaggata gcagtaaaaa taaaaacaca agttatcaca
     4381 aatcactcct tgttctgaac cggcacacaa aactgtcata tacgtttcaa aagtaaaaac
     4441 acactgctgc cacaagattc acgacgtaac cgggcagcat atcctgatta cggacaatcc
     4501 atgaaaccgc ctcgccagaa gcgtccatca catccggttt ttccctgttt tatattccac
     4561 gaaccatttt attttcagga atctccgggc ctttatcccg catcatatac acagctatga
     4621 caacccgctc ttctgcagtc atcatttccc cgtggcttcg cggattttct gttccgtctt
     4681 atactgcgaa agcgcataca cggcccatat tgttgccggt atccagccca gcagcgttat
     4741 ctgtaacacc agacaaaaaa tccccgtcag aggccggccg atggtgaaaa aagtcagcca
     4801 gggaagaaga aaagcaatca acagacgcat aaaaactcct tatgattatg aaattaacga
     4861 atcagaggct gcgtttaaca ttaccggaaa taagctgtcc tgcttcacaa cctccctttt
     4921 ctgaccagaa gcatcccctt gtacaacaac tcaacttcat tatgtacccg taatctcttt
     4981 atcagattct gcttatgagt ggagaatgtt ttctctgaca tgccaagata tcgtgccccc
     5041 tctgttccgt tactgccata aagcagcatc atcagtacat ttacctgccg gcctgacagg
     5101 gtcagcttct ccaccgacat gaatgcgcgg tacagatgac tgatatcgtt tactgaacat
     5161 cgctcagtaa gatgtctgat atcataaacc ggactgtcaa ccactcccca gagaaaacgg
     5221 agcgcttccg gagaagaaaa ataaagggtg ttcatatcac ttatattatt cttatacaga
     5281 aagccgacaa tttcagatat ccgggaagaa gatattatca ccacattatc tgtctgcatg
     5341 ccgctgataa tgaatttacc gggattaata tactccttca ctccctccag aaaataactg
     5401 ttatcacaaa taaaacgcat acccgttcca catgaaattt atcacgcctg accacataac
     5461 attaattatc cggagcaccg ggatgcccgt gacacagcat gcacagaaat tcccgtcgca
     5521 acagccttca tcagaccggc tttttaccgg tctgcggtgc tgcagagata tttaacactg
     5581 acacctcgca gaccgctttt ctccatcgca ttgtaaagat gcgcacatac cgtcttcatg
     5641 cttatcccca gctggtctgc cagaaaggta cagccgtact catggaaaat aaccccgaag
     5701 accaccacca tctcggtacg ggtcagtggt cttgccgtat gtccgaacag ccacgc
//
LOCUS       DAZGXZ010000235         5701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000235 DAZGXZ010000000
VERSION     DAZGXZ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.236"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(309..557)
                     /locus_tag="ACHKJF_005140"
     CDS             complement(309..557)
                     /locus_tag="ACHKJF_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0952339.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
     gene            776..2362
                     /gene="prfC"
                     /locus_tag="ACHKJF_005141"
     CDS             776..2362
                     /gene="prfC"
                     /locus_tag="ACHKJF_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP0952340.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            2755..3360
                     /gene="osmY"
                     /locus_tag="ACHKJF_005142"
     CDS             2755..3360
                     /gene="osmY"
                     /locus_tag="ACHKJF_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP0952341.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            3487..3648
                     /locus_tag="ACHKJF_005143"
     CDS             3487..3648
                     /locus_tag="ACHKJF_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP0952342.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            3770..4843
                     /locus_tag="ACHKJF_005144"
     CDS             3770..4843
                     /locus_tag="ACHKJF_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP0952343.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            4840..5622
                     /locus_tag="ACHKJF_005145"
     CDS             4840..5622
                     /locus_tag="ACHKJF_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0952344.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVLDVLCELRPEPEDEIAEVLLNNTYAVFSVSG"
BASE COUNT         1412 a         1388 c         1516 g         1385 t
ORIGIN      
        1 ataaacacga agacggtgaa cacctttctg tatatgggac ttggtaaaaa cggtcttaag
       61 tacaggagtg cgaagcacct ggttggacgt gcgtagccgg aatgcatatt cgtgaagtcg
      121 ggtaatgaga gttgaacggt ggcagtgaga atggagcagg aaaaaatcag atgcggaaag
      181 cgaaaataca cagcgggtgc gatgtgcttc atgctgtttg tatggctggg tggtgtggta
      241 acactctgtg tacttgctgc actgataaca caataaaaga ggggagctgc cagctccccg
      301 tgctgatgtc accggataaa ccagtcatcc gctgtttccc agacttcctg cagcgtttcc
      361 tgtacaaatt cttgcgcgga ttctttatcc acggctctta aaattgtgag tccatcatta
      421 ctggcagatt tcacaatcac atctacatca tcataacgct taccgacacg tcgtagcatt
      481 tcctgttgca gggcaggaac agagcctttt ggcattttgc agattttttc tttagcgata
      541 cagatctcaa tacgcataac acctcctgtc actgtgtttg tatacagtgt tatttttaac
      601 tgtatgaata aacagtgtca atagagcaaa gattgctgca tatacacatg ttctttggct
      661 acaaatacac ctactgatca tgttctgcta tgctctgata cctccaaaat gtacaatcag
      721 cggcatgaat ttttctttgt acatatgatt gtacataatc aaagagacaa caattatgac
      781 aagccttttg gccgcagagg tagcgaaaag aagaaccttt gcaattattt ctcacccgga
      841 cgccggtaaa actaccatca ccgagaaggt gctgctgttc ggacaggcca ttcagaccgc
      901 tggtacagta aaaggccgtg gttccaacca gcacgctaag tcggactgga tggagatgga
      961 aaagcagcgt gggatctcca ttactacgtc tgtgatgcag tttccgtatc acgattgcct
     1021 ggttaacctg ctcgacaccc cggggcacga agacttctcg gaagatacct atcgtaccct
     1081 gacagcggtg gactgctgcc tgatggttat cgacgctgca aaaggtgttg aagatcgtac
     1141 ccgtaagctg atggaagtta cccgtctgcg cgacacgccg atcctcacct ttatgaacaa
     1201 acttgaccgt gatatccgcg acccgatgga actgctcgat gaagttgaga acgagctgaa
     1261 aatcggctgc gcaccgatca cctggccgat tggctgcggc aagctgttta aaggcgttta
     1321 ccacctttat aaagacgaaa cctatcttta tcagagcggt aaaggccaca cgattcagga
     1381 agtccgtatt gttaaagggc tgaataaccc ggatctcgac gctgcggttg gtgaagatct
     1441 ggcacagcag ctacgtgacg aactggaact ggtgaaaggc gcgtctaacg agttcgacaa
     1501 agaattgttc cttgcgggcg aaatcactcc ggtattcttc ggtactgcgc tgggtaactt
     1561 cggcgttgat catatgctgg atggcctggt ggagtgggct cctgcgccga tgccgcgtca
     1621 gactgatacc cgtaccgtag aggcgagcga agacaaattt accggcttcg tatttaaaat
     1681 tcaggccaac atggacccga aacaccgcga ccgcgtggcg tttatgcgcg tagtatccgg
     1741 taaatatgaa aaaggcatga agctgcgcca ggtgcgtact gcgaaagatg tggttatctc
     1801 cgacgcgctg acctttatgg cgggtgaccg ttcgcacgtt gaagaagcgt atccgggcga
     1861 tatcctcggc ctgcacaacc atggcactat tcagatcggc gacaccttta cccagggtga
     1921 gatgatgaag ttcaccggta ttccgaactt cgcaccagaa ctgttccgtc gtatccgcct
     1981 gaaagatccg ctgaagcaaa aacagctgct caaagggctg gtacagcttt ccgaagaggg
     2041 cgcggtgcag gtgttccgtc cgatctccaa caacgatttg atcgttggtg ctgttggtgt
     2101 gctgcagttt gatgtggtgg tatcgcgcct gaagagcgaa tacaacgttg aagcagtata
     2161 tgaatcagtc aacgttgcca ctgcccgctg ggtagaatgt gcggacgcga agaaattcga
     2221 agagttcaag cgtaagaacg aaagccaact ggcgcttgat ggcggcgata acctcgctta
     2281 catcgctacc agcatggtca acctgcgcct ggcgcaggaa cgttatcccg acgttcagtt
     2341 ccaccagacc cgcgagcatt aattcttgcc tccagggcgc ggtagccgct gcgccctgtc
     2401 aatttccctt ccttattagc cgcttacgga atgttcttaa aacattcact tttgcttatg
     2461 ttttcgctga tatcccgagc ggtttcaaaa ttgtgatcta tatttaacaa agtaatgaca
     2521 tttctgacgg cgttaaatac cgttcaatgc gtagatatca gtatctaaag ccgtcgattg
     2581 tcattctacc gatattaata actgattcag aggctgtaat ggtcgttatt catcacccat
     2641 cgcttttgtg atggcgacca ttgacttctg tagagggtga agtctctccc tattcagcaa
     2701 tgcaacctcg tgttgccagg ctcaaattac gagcaaacat acaggaataa atcgatgact
     2761 atgacaagac tgaagatttc gaaaactctg ctggctgtaa tgttgacctc tgccgtcgcg
     2821 accggctctg cctacgcgga aaacaacgca cagactacca atgaaagcgc agggcaaaaa
     2881 gtcgatagct ctatgaataa agtcggtaat ttcatggatg acagcgccat caccgcgaaa
     2941 gtgaaggcgg cgctggtgga tcatgacaac atcaagagca ccgatatctc tgtcaaaacc
     3001 gatcaaaaag tcgtgaccct gagcggtttc gttgaaagcc aggctcaggc cgaagaggca
     3061 gtgaaagtgg cgaaaggcgt tgaaggggtg acctctgtca gcgacaaact gcacgttcgc
     3121 gacgctaaag aaggctcggt gaagggctac gcgggtgaca ccgccaccac cagtgaaatc
     3181 aaagccaaac tgctggcgga cgatatcgtc ccttcccgtc atgtgaaagt tgaaaccacc
     3241 gacggcgtgg ttcagctctc cggtaccgtc gattcccagg cacagagcga ccgtgctgaa
     3301 agtatcgcca aagcggtaga tggtgtgaaa agcgttaaaa atgatctgaa aactaagtaa
     3361 ttcgtcgtaa ttcgtcctcc cgaactttgt cgggaggcgc gatgtgcacc acactaaaaa
     3421 tatcgcattg agtgattttc acgctcatat caagcggtga cattaactat ggtaaaggag
     3481 acgcttatgt ttcgttgggg catcatattt ctggttatcg cgttaatcgc cgccgcactt
     3541 gggtttggtg gtctggccgg taccgctgca ggcgcagcta aaattgtctt tgtcgtcggg
     3601 attattctgt tcctggtgag tttgttcatg ggccgaaaac gcccctagat ttcagaccat
     3661 taagaaaatt ttatccaaag ccagtccagc ggactggctt ttgcggtttt agcgaataat
     3721 aaattgcgtt actttgtcat tctttcacaa caaaaacagg aaggcagagg tggggcagcg
     3781 aatacctgta acgcttggta atattgcgcc gttgtcgcta aggccgttcc agcctggacg
     3841 aatagctctg gtgtgcgaag gcggcggaca gcgtggaatt ttcacggctg gcgtgctgga
     3901 tgagtttatg cgcgcgcagt ttaatccttt cgatctttat ctcggcacat ctgccggggc
     3961 gcagaacctc tcggcgttta tctgcaatca gcccggttac gcgcgcaaag tcatcatgcg
     4021 ctataccaca aaacgcgaat ttttcgatcc attgcgcttt gtccgtggag gaaatcttat
     4081 cgatctcgac tggctggtgg aggccaccgc aagtcagatg ccgttgcaaa tggataccgc
     4141 cgcgcggttg tttgacagcg gcaaatcgtt ttatatgtgc gcctgtcgtc aggatgacta
     4201 cgcgccgaat tactttttac caaccaaaca aaactggctg gatgtgattc gcgcctccag
     4261 tgcgatacct ggcttttatc gtagcggagt gtcgctggaa ggcattaact acctggatgg
     4321 cgggatcagt gatgcgattc cggttaaaga ggcggcaagg cagggcgcta aaacgttggt
     4381 cgtcattcgc actgtgccgt cacaaatgta ctacacgccg cagtggttca aacgcatgga
     4441 acgctggctg ggtgacagta gcctgcagcc gctggtcaat ctggtgcagc atcatgaaac
     4501 cagctatcgt gacattcagc aatttattga gaaaccaccg ggcaagctgc ggatattcga
     4561 aatttatccg ccgaagccat tacatagtat cgcgcttggc agtcggattc cggcgctgcg
     4621 cgaagactat aaacttgggc gtttatgcgg tcgttatttc ctcgccacgg tcggtaaatt
     4681 attgactgaa aaagcgccgc ttacccgcca tctggtgcca gtggtgacgc cggaatcgat
     4741 tgtcattccg cctgcgccag tcgccaacga tacgctggtt gccgaagtga gcgatgctcc
     4801 gcaggcgaac gacccgacat ttaacaatga ggatctggct tgatttgccg ttttatcgac
     4861 acccactgcc attttgattt cccgccgttt agtggcgatg aagaggccag cctgcaacgc
     4921 gcggcacaag cgggtgtagg caagatcatt gttccggcga cagaggcgga aaattttgcc
     4981 cgtgtgcagg cgttagcgga aaaatatcaa ccgctgtatg ccgcattggg cttgcatcct
     5041 ggtatgttgg aaaaacatag cgatgtgtct cttgatcagc tacagcaggc gctggaaagg
     5101 cgtccggcga aggtggtggc ggtgggggag atcggtctgg atctcttcgg cgacgatccg
     5161 caatttgaga ggcagcagtg gttacttgac gaacaactga aactggcgaa acgctacgat
     5221 ctgccggtga tcctgcattc acggcgcaca cacgacaaac tggcgatgca tcttaaacgc
     5281 cacgatttac cgcgcactgg cgtggttcac ggtttttccg gcagcctgca acaggccgaa
     5341 cggtttgtac agctgggcta caaaatcggc gtaggcggta ctatcaccta tccacgcgcc
     5401 agtaaaaccc gcgatgtcat cgcaaaatta ccgctggcat cgttgttgct ggaaaccgac
     5461 gcgccggata tgccgctcaa cggctttcag gggcagccta accgaccgga acaggctgtg
     5521 cgggtgttgg acgtgctttg cgagttgcgc ccggaaccag aagatgagat tgccgaagtg
     5581 ttgcttaata atacgtatgc ggtgtttagc gttagtgggt agtttgccct tcgtaggcag
     5641 catccggcag tcggcgcata atgcctgatg cgacgcttgt cgcgtcttat catgcctaca
     5701 g
//
LOCUS       DAZGXZ010000236         5534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000236 DAZGXZ010000000
VERSION     DAZGXZ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.237"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            145..321
                     /locus_tag="ACHKJF_005146"
     CDS             145..321
                     /locus_tag="ACHKJF_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952345.1"
                     /translation="MADFGSTKYNASFEEWHELLMDYAELRGGSAADAEAWRDDYEAG
                     KTPVEAYCDEWGDE"
     gene            318..677
                     /locus_tag="ACHKJF_005147"
     CDS             318..677
                     /locus_tag="ACHKJF_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952346.1"
                     /translation="MSEINYQEGHEKAGQAKPVAWRYRYVKKGVTDFQGKQWAGDWKY
                     VQTKEDCNDRPNYEIQALFTAPPAPVTSEGLVKAVCFYEQVKRENPPVETGAWKDAVD
                     WVLKEACQAVSISNKGG"
     gene            681..857
                     /locus_tag="ACHKJF_005148"
     CDS             681..857
                     /locus_tag="ACHKJF_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952347.1"
                     /translation="MRVVCISLLPYPTRFLASALIAKPRVLMDDSIIPTPKRRHTGIA
                     AARRAAKKRRRAKR"
     gene            1050..1228
                     /locus_tag="ACHKJF_005149"
                     /pseudo
     CDS             1050..1228
                     /locus_tag="ACHKJF_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1562..1906
                     /locus_tag="ACHKJF_005150"
     CDS             1562..1906
                     /locus_tag="ACHKJF_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952348.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDNDIIRTYKMDIAVLE
                     IALASLAAEPAGKLHEYKPVGHQRLVDELTMLVKQLTWQLRKTKPDCKLPGKAMDYLK
                     RNGLISAEDVLR"
     gene            2198..2353
                     /gene="hokD"
                     /locus_tag="ACHKJF_005151"
     CDS             2198..2353
                     /gene="hokD"
                     /locus_tag="ACHKJF_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0952349.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TVYEPEE"
     gene            2519..3169
                     /locus_tag="ACHKJF_005152"
     CDS             2519..3169
                     /locus_tag="ACHKJF_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952350.1"
                     /translation="MNENQLAPCWEFQPYLAESNVRQLLAEIANVLEQLYYHKHVLDS
                     NWSEGVRAYDWVRNHLIQNEGAIPGLKMISKGLDYVVALNKVPLQFTKDCINNPKKKH
                     RLRRNKVEHEQLSLFGDVEAEQDITWRVIAEPFLSEEGDGELESTLPRWEVALVGFNI
                     YGAQISMVSHQSTASMPLMPLDGNTLPGEAEINKVSLRRRTKDKILDVSSNGTSGE"
     gene            3150..4253
                     /locus_tag="ACHKJF_005153"
     CDS             3150..4253
                     /locus_tag="ACHKJF_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952351.1"
                     /translation="MEHQVNNFTEYRGDKLKLARMAIGLSCEELAEKIGKTKQFVSKL
                     EKGFRPSEQCLELIASALMIKPDFLFTERKYALESDVCHFRSKKSRTQTLTNSVLARA
                     EILNIIISAVEGEIEFPDVNIPEHPGTELLTPNDIERVAEDCRRAWNLGLGPISSMVK
                     LAESLGVIVAHVTGVDDRVDAFTVHNNRPVIIRNNVKKSICRFRSDLGHELGHLVMHE
                     GITTGDKLTESQADHFSSALLVPRLSFIKEFPRIRGKQFDWSALVEFKLRWKISLKMC
                     IYRASALGLLTQEQARTGYMHLNSRGYTRVEPGDELLRHEEPSMLSEAIDMLDDATWL
                     RILMKTGLSQDLIRELFSINRPITNPRNIFQIV"
     gene            4356..4634
                     /locus_tag="ACHKJF_005154"
     CDS             4356..4634
                     /locus_tag="ACHKJF_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952352.1"
                     /translation="MAHNTKSHNSDNSAVFASRHGRRSRTFKSDWFQHDPCTEEQAEW
                     LIQCYRRRGYEFQKDLSLDRLHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            4636..>5534
                     /locus_tag="ACHKJF_005155"
     CDS             4636..>5534
                     /locus_tag="ACHKJF_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0952353.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGHESMPVFQNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGAGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADAFPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIAPPSHARQQQNPPQEKTVVCITVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGQPADDPHHLIGHGQGGMGT"
BASE COUNT         1561 a         1156 c         1474 g         1343 t
ORIGIN      
        1 gaatagctgg tggagatttt gaggatgtat tcgaagtaga tgatgacgca acacctgatg
       61 aaattcatga cgaagcaaaa gaaattttct ttaactactg caattactca taccacgaaa
      121 taaaagatga agaggaagga caaaatggct gattttggtt caactaaata taacgccagt
      181 tttgaagaat ggcatgaact gttaatggat tatgcagagt tacgcggtgg aagtgccgct
      241 gatgctgaag catggcgtga tgattatgaa gcagggaaaa ctccggtcga agcatattgt
      301 gatgagtggg gcgatgaatg agcgagatta attatcagga agggcatgaa aaggcagggc
      361 aggcaaaacc agtggcatgg cgatatcgct acgtgaaaaa aggcgttaca gactttcagg
      421 ggaagcagtg ggctggtgac tggaaatatg tccagacaaa agaagattgt aacgacagac
      481 cgaactatga aattcaggcg ttattcacgg ccccgccagc cccggtgaca tcagaaggac
      541 tagttaaagc cgtgtgcttt tatgaacagg taaagcgtga gaatccacca gtcgaaacag
      601 gtgcatggaa agatgctgtt gactgggtac tcaaagaggc ttgccaggct gtaagcatta
      661 gcaacaaagg ggggtgagta atgcgtgtgg tatgtatcag tttgttaccg tacccgactc
      721 gttttttggc ttctgcgcta attgcaaagc cgcgtgtcct gatggatgac agcatcatcc
      781 cgacaccaaa gcgccgccat accggtattg cagcggcacg acgagcagca aagaaacgca
      841 ggagagcaaa acgatgaaaa accgtaaagc aaaaattctg ttagctcgca gaaacggtgt
      901 tggtgtctgg cggtgggtga ggattagtaa cagacgggtg aggttgacgg ggtgttgcgg
      961 tgttatgggg cacagttgtt gcaaaaagcc cagcgcggcg caaaaccgct ggaaaaacca
     1021 ctgttatctg cgcactaaag gagagtgaga tggcgttaac acaccgcgaa ctctgtcaga
     1081 ttgcgtacaa gttccttaag cgcaacgggt tcaaggtttg ctttcatgac cgctttatag
     1141 ctgtaaccag taccggagaa cagccagatg ctatgggatt cagaaattca gcatcatgcc
     1201 tgatagaggc gaagtgttct cgtgctgact tgttggcaga tagaaaaaag cgtttccgta
     1261 aaaatccctc tcttggcatg ggcgactgga gattctttat tagtgagccg ggaattattt
     1321 caattgagga tttaccacct ggctggggat tacttcacgt tgttaacgga agagtacgga
     1381 aagtacatgg gtggcccaag ggtaattgct gttggggtaa tcctgacgat aagccattta
     1441 ctggaaataa gcaggttgaa tgcgattaca tgttgtctgc attaaggcgc atggagttga
     1501 gagggcacct taatgaaata tatgacggtg taattgttaa taagaaagaa ggaaacgcgg
     1561 catgacgact tttaccagag agcagttaat agctcacgca gaggagacta ttgaagcaca
     1621 gagactgtgc ataccgggca caatcgacaa tgacatcatc cgcacatata agatggatat
     1681 tgctgttctg gaaatcgcac tggcatcgct ggcagcagag ccagccggta aattgcatga
     1741 atacaaacca gtgggacatc agcgtctggt cgacgagtta accatgctgg taaagcagtt
     1801 aacctggcaa ctgaggaaaa cgaagccgga ctgtaaattg ccgggtaagg cgatggacta
     1861 cctgaagcga aacggactga taagtgcaga ggatgtttta cgatgacctg gcatggggca
     1921 ttcacaacgg tagggattgc aatggcggtg gtgctgattg tgtattcgat ttgtcgctgg
     1981 gggtaaaaac gatttgcggg gaaaggatag ttaagtagaa ttgctgcggg tgcttgaggc
     2041 tatctgtctc aggcatgaac accaaaaggc agatagagaa aagccccagt taacattacg
     2101 cgtccggcaa gacgcttaac attaatctga ggccatatct atgctctaca cacgtaggtt
     2161 agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa aggcgatgct
     2221 aatcgccctg atcgtcatct gtttaaccgt cattgtgacg gcactggtaa cgaggaaaga
     2281 cctctgcgag gtacgaatcc gaaccggcca gacggaggtc gctgtcttca cagtctacga
     2341 acctgaggag taagagacca ggcgggggag aaatctcccg ccacctctga tgtgtcaggc
     2401 atcctcaacg cacccgcact taacccgctt cggcgggttt ttgcattagt ctggttgaca
     2461 aaaacaggaa aatgcgaaaa tataaggttt acgaattcta aaaaaagcga aacttgaaat
     2521 gaatgaaaat cagttagctc cttgttggga atttcaacct tatcttgctg aaagcaatgt
     2581 tcgccagttg ttggcggaga tcgctaacgt acttgagcag ttgtactatc ataagcacgt
     2641 attagacagc aactggtctg aaggtgtaag ggcttatgat tgggtcagaa accatcttat
     2701 tcaaaatgaa ggcgcaattc ctggtcttaa gatgatttcc aaggggttgg actatgtagt
     2761 tgctttaaat aaagttccgc tacaatttac caaagattgc attaataacc ccaaaaagaa
     2821 acatcgcctg cgtcgaaata aagtagagca tgagcagctt tcattgtttg gtgatgttga
     2881 ggctgagcaa gatattacat ggcgagtcat agcggagcca tttttatccg aagagggaga
     2941 tggtgaatta gagtccacac tgcctcgttg ggaggtggct cttgttgggt ttaatatcta
     3001 tggtgctcag attagtatgg tttctcatca atctacagca tcaatgccac ttatgcccct
     3061 tgacggtaac acactccctg gcgaagcgga gattaataag gtgtctcttc gtcggcgtac
     3121 gaaggataaa attttggatg tgagcagtaa tggaacatca ggtgaataac ttcactgagt
     3181 atcgaggcga taagctcaaa ctagcaagaa tggctattgg gctttcatgt gaagagttgg
     3241 ccgaaaaaat tggcaaaaca aaacaattcg ttagcaaatt ggagaaggga tttaggccat
     3301 cagagcaatg cctggagtta atagcctcag ctcttatgat taagcccgat tttctattta
     3361 ctgagcgaaa atatgctctg gaaagcgatg tttgccattt tcggagtaag aagtccagga
     3421 ctcaaacgct gactaatagt gtcttggcca gggctgagat tcttaatatt ataatttctg
     3481 ctgttgaagg tgaaatcgaa ttccctgatg tcaacatacc agagcaccca ggaactgaat
     3541 tacttacccc gaatgatatt gagcgagtgg cagaagattg tcgccgtgca tggaatctag
     3601 gtcttggccc tatatcatca atggtgaaat tggcggagag tttgggggta atcgttgcgc
     3661 atgttacggg agtcgatgat cgtgttgatg cattcactgt tcacaataac aggcctgtta
     3721 ttatcaggaa caatgtaaaa aaaagcatat gtagatttcg ctctgattta ggtcatgagt
     3781 tagggcattt agtaatgcat gagggcataa ctactggtga taaacttacg gaatcacaag
     3841 ctgatcactt ttctagcgcc ttattggttc ctaggctatc tttcattaaa gaatttccac
     3901 gaatacgagg taagcaattc gattggagtg ctctggttga atttaagctt agatggaaaa
     3961 ttagccttaa aatgtgtatt tatcgagcca gtgcattagg actattgacc caggaacagg
     4021 caagaactgg ctatatgcat cttaattcta gagggtacac gagagttgaa ccaggtgatg
     4081 aacttttgcg ccacgaagaa ccaagtatgc tgtccgaagc gattgacatg ctggatgatg
     4141 caacttggtt aagaattctt atgaaaactg gcttgagtca agatttaatt cgtgagttgt
     4201 tctccatcaa tcgacctatt acaaatccaa gaaatatttt tcagattgtt tgagcatacc
     4261 cgctacggcg ggttttgttt tttccgggct ttttggttta caatccgcac gccagcctga
     4321 acaactggca cctgctgcgc cagcagagaa aaacgatggc gcacaatacc aaatcacaca
     4381 attctgataa ttcagctgtc tttgccagca ggcacgggcg gcgttcccgc acattcaaat
     4441 ctgactggtt ccagcatgac ccatgcacag aagaacaggc cgaatggtta attcagtgct
     4501 accgcaggcg cggatacgag tttcagaaag acctcagcct cgaccgactg cactggataa
     4561 tctctgtgag gctcccttac tccgagcgcc caccgcgtcc atcccgcaca ttccagcaac
     4621 gtatctggag gtaacgtgcg ggtattactt cgacctgttc ttgtgccaga actcgggctg
     4681 gtgatcgtta agccgggcca tgaatccatg ccggtattcc aaaatacccg ggtactggtg
     4741 gagccggaac cgaaaagcat gcgtaatctg ccgtccgggg tcgttcctgc cgttcgccag
     4801 ccgctggcgg aggataaatc attactgcca tttttcagcg acgaacgagt gattcgtgct
     4861 gctggtggcg ctggcgcatt gtctgactgg ttactgcgcc atgttaaatc ctgccagtgg
     4921 ccacacggcg attatcacca cagtgaaacc gtcattcacc gttatggcgc tggcgcgatg
     4981 gtgttgtgct ggcactgtga caaccagctg cgtgaccaga catccgaatc actcgggcaa
     5041 cttgctcatc aaaacctgtc agcatggatg attgacgtca tacgccatgc aatgaatggc
     5101 acgcaggagc gggaattgtc gctggctgaa ttatcctggt gggcggtctg caatcaggtg
     5161 gcggacgcgt ttccggaggc agtattacgt cgttctctgg ggttacgtgc ggaaaaaatc
     5221 cgctcggtgt atcgcgaaag cgacatcata ccgggagagc agaccgccac cagcatactg
     5281 aagcagcgca caaaaaatat tgcgccgccg tctcacgccc gccagcaaca gaacccacca
     5341 caggaaaaga cggtggtatg catcaccgtt gatccggagt ctccggaatc ttttatgagg
     5401 cgacctaaac gtcgccgttg ggtaaatgag aaatatacgc gctgggtgaa gacacagccg
     5461 tgtgcgtgtt gtggtcagcc agccgacgat ccccatcacc tgattggtca cggtcaggga
     5521 gggatgggaa caaa
//
LOCUS       DAZGXZ010000237         5473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000237 DAZGXZ010000000
VERSION     DAZGXZ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.238"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1165)
                     /locus_tag="ACHKJF_005156"
     CDS             complement(join(<1..907,907..1165))
                     /locus_tag="ACHKJF_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0952354.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVA"
     gene            complement(1239..2381)
                     /locus_tag="ACHKJF_005157"
                     /pseudo
     CDS             complement(1239..2381)
                     /locus_tag="ACHKJF_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
     gene            complement(2326..3834)
                     /locus_tag="ACHKJF_005158"
     CDS             complement(2326..3834)
                     /locus_tag="ACHKJF_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0952355.1"
                     /translation="MAILDDVIGVFSPGWKAARLRSRAVIQAYEAVKTTRTHKARREN
                     RTADQLSQYGAVSLREQARYLDNNHDLVIGVFDKLEERVVGKNGIIVEPHPVLRNGAI
                     ARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLMLRTWLRDGEVFAQMVSGRINSLAP
                     SAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDDWGRPEKYLVYKSRPVSGRQMETKE
                     VDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSALKEYEDSELTAARIAAALGMYIRKG
                     DGQSYEPDGNGRKENERELTIQPGIIYDDLKPGEEIGMVKSDRPNPNLETFRNGQLRA
                     VAAGSRLSFSSTARNYNGTYSAQRQELVESTDGYLILQDWFIGAVTRPMYRAWLKQAV
                     ASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKEAEAWKIQIRGGAATESDWVRAGGR
                     NPDDVKRRRKAEIDENRNLDLVFDTDPASDKGGSSAATKRQEPQHTDDQSEE"
     gene            complement(3834..4046)
                     /locus_tag="ACHKJF_005159"
     CDS             complement(3834..4046)
                     /locus_tag="ACHKJF_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952356.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            complement(4043..5158)
                     /locus_tag="ACHKJF_005160"
                     /pseudo
     CDS             complement(4043..5158)
                     /locus_tag="ACHKJF_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            complement(5246..5368)
                     /locus_tag="ACHKJF_005161"
     CDS             complement(5246..5368)
                     /locus_tag="ACHKJF_005161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952357.1"
                     /translation="MTEAEILGLIHRVTGIRHKRTPIIIQLLINIAAFVSFFIV"
BASE COUNT         1236 a         1576 c         1308 g         1353 t
ORIGIN      
        1 gccacggttt tacagtaaaa atggtatctg ttctctgact cttccggcgt cagcccaccg
       61 ttataatggt gaggtctgac gctgttgtag tagttcaata tgtaatcatt aatttgctgc
      121 cgggcctcgt ccttgcctgc gtaaccattc gttggcaccc attctgtttt cagactgcgg
      181 aagaagcgtt ccatggggct gttatcccag cagtttcccc gccgactgac gctttgattt
      241 attctgcaac gccagagaag ttgttgatat ttaaggcctg tatactggct tccctggtcg
      301 ctatggaaca cgacatcccg gggttggcca cgcgtctcat aggccatccg cagggcactg
      361 cttatcagtg cggtatcggc atgcgctgac agactccagc cgataaccct gcgggcaaaa
      421 agatccataa caaccgccag atagcaccag cgatttcctg cccagagata cgtaatatct
      481 ccacaccata cccggtctgg ctccggtact gcgaactgac gctcaagcag attcggcagg
      541 ctggtatgct cctgacgggc atttttatac tgatgttttc cgggctgaca actgctcagg
      601 ttcagatatt tcatcagacg tccggcacgg taacggctca tcgggacgcc gttttgagtc
      661 agcatttcag ccagagtgcg cgcgcccgca gagccccgac tttggttcca cgcccggcgt
      721 atttcgctgc acaacctgac tcgtgccgga ttaaccgtat cgcgtcgttt tcgccagtac
      781 cggtaactgc tgcggtgtat ttccagagca gaacacaggc tgacaaccgt gtggctgtca
      841 cttagtctgg cggctatcgt gaaccgttca gcgagtcgga cattaagagc gcggtagcct
      901 tttttaatat cgtattttgt tcctccagac ggcgaacttg cttttccagc tcgcggatac
      961 gttgctggtc tggagtaatg ggtgtggcag agggcgtaat accctggcgc tctcgcctga
     1021 gctggcgtac ccagctctca agcgtggttg aaccgacatt catcgcttca ctggcctgtc
     1081 gatatgagta gcccttatca acaatcagct gtgcacattc cagcctgaac tcaggggtga
     1141 aggttcgttt ggttttcttg ttcattaagt cacctgtttt gtgttgtggt gagaatatca
     1201 cctttcatca ggtggccaaa tttagtgtgc cactacacac cgaccatctg catcgggtta
     1261 taactggaaa caccaatacc acgttcagtc agtgacatac gggcatattc acgcagggtc
     1321 atcccgttgt agacattatc acgttcagtt ttttcaaacc cggcgcgcgc catcagcgcc
     1381 tggcggatcc cgtcccccac aaaattaccg ttaccggcat aaatatgagc cggggtattt
     1441 ttattggatg gcgtggactc gcgccccatc tcgttcaaca gcttttcgcg ggcctgctcc
     1501 agcgaacatt caggatcggc aagacactga gcctgcaacg actgataacg cccgccaaac
     1561 atggcaaaca gatcattaat accgtttaca cgcgcttttt gctctgccag gacctgcgca
     1621 cggatgctgt tttcatccac cacgggtgct gctgcctgca ctggcgtccg ggaggctgca
     1681 ggtccatcat cctgtacgcg tggagcactg ttgcgtggcg gagtaatcat atttcgaatg
     1741 gattccggca tctttttaaa ttcctctgta cgttttgact gaatacatgc cattgcctta
     1801 acggctggtg tcacctgatc agcaaatccc tgtgccagac attcggcacc ggacatcctg
     1861 gtctcatccg ccagcatggc agcaatttca tcggtggttt tcccggtttt ctgtgcataa
     1921 gcgggtaaca gaaccgcctc aactctatcg agcaggtcgg cataggtgcg catgtcctcc
     1981 gcatcaccgc ccgtaaagcc aaatggttta tgaatcatca tgaaggtgtt ttccggcata
     2041 atgaccgggt ttcctaccat cgcaatgacc gacgccattg acgccgccac accgtcgaca
     2101 taaacggtaa tggacgcacc atgtgttttc agcgcattaa aaatggcgat gccttcaaag
     2161 acatcgccac ccggtgaatt aatatggaga ttaatgtggg tgatatcgcc cagtgcattc
     2221 agatcactga taaactgctt cgctgtaaca ccccagaaac caatctcgtc ataaatataa
     2281 atatccgcgt cactctggtg accagcctgc atcctgaacc aggaattatt cttcggactg
     2341 gtcgtcggtg tgctgcggct cctgtcgttt cgttgcggca ctactgcctc ctttatcact
     2401 ggccggatcg gtatcaaata ccagatccag attgcggttt tcatcaattt cggccttgcg
     2461 ccgacgtttg acatcatccg gattacgacc accagcacgt acccagtctg attctgtcgc
     2521 cgctccacca cgaatctgga ttttccaggc ctcagcctcc ttaacagggt caatccacgg
     2581 catcactggt ccggaataca ccgcggtata cagtgaagaa cggtcaagat cgcggggtag
     2641 cctgataaca ccggatgcca cagcctgttt cagccatgca cgatacatcg ggcgggtgac
     2701 ggcaccaata aaccagtcct gcaggatcag gtagccatca gtggattcaa ccagctcctg
     2761 acgctgggcg ctgtaagtgc cgttatagtt gcgcgctgta ctggaaaaac tcagacgact
     2821 gcccgccgcc acggcacgca actgaccatt acgaaaagtt tcaaggttag gattggggcg
     2881 atccgacttc accattccga tttcttcgcc gggtttcaga tcgtcgtaaa taatgcccgg
     2941 ctgaatggta agctcacgtt cattctcctt tctgccatta ccatccggtt catagctctg
     3001 cccgtcgcct ttccggatgt acatccccag agcagcggcg atccttgctg cagtcagctc
     3061 agaatcttca tattctttca gggcactgag gcggatcagc acaccggaca acaaagacgt
     3121 cccgcgcatc tggtgcagac ggcgaacaaa tttaagatgc agcattcgct ctgcatccac
     3181 ttctttggtt tccatctgcc gtccggatac gggacggctt ttatacacca gatatttttc
     3241 gggacgcccc cagtcatcaa caaacacgcc ctgattcagc ctgttgctct catcactggt
     3301 catgggaata aagtccggct cgagcgcctc cagccagaaa tgaacaccgg cagaaggcgc
     3361 caggctgttt atgcgcccgg aaaccatctg ggcaaacacc tcaccatcgc gcagccaggt
     3421 acgaagcatc agacgttcca gcatcggacg ggtaaactgc ccggtgactt ccgggctgac
     3481 agaccattca ctccatcggg tgcgaatctc cgcagccagg tcacgggcaa tggccccatt
     3541 gcgtaatacc ggatgtggct cgacaataat cccgtttttc cccaccaccc gttcttccag
     3601 cttgtcaaat acaccaataa ccagatcgtg gttgttatca aggtaacggg cctgctcacg
     3661 taacgacacg gccccgtact ggcttaactg gtcggcagtt cggttctccc gtcgggcttt
     3721 gtgtgtccgc gtcgttttta cggcctcata agcctggatc accgcacggg aacgcagcct
     3781 tgccgctttc catcctggtg aaaaaacgcc aatcacatca tcaagaattg ccatcagaac
     3841 ctcgccagcc ggtacccggg atgcccccgt cgtcgtgtaa tcagagccgc aaggcggcgc
     3901 tcccactcct gccgtccctg ccggatctca gataagtttt ccatggtcat ctgctgacca
     3961 ttaaaggtga cggattttcc gtccagcacc gccatttcag cttccgtata acgctgaatc
     4021 atggcttcga tatcattctg gttcataacc atcctccgga agtcagccag gggttaacat
     4081 cgtcagttac tgttttcttc cgtttttgtt ttttaacagg cgtggatacc ggttccggtg
     4141 aggatgacgg ttcggtactg tcctggacac actccagcca ggtctcccgg ctcgcccact
     4201 ccggtgcatc cggccagcgg atcttttcgt atccatgcag aatgaccaga gcctcggcat
     4261 acaccatcag gtcaaaagct tcgttggcac cgcgacccgg cttactccat ttcccgtcac
     4321 tgctccgctc ttcatacgtc agttcgtcgt aaaaccagct ccccagccag tcagggaaat
     4381 gcacatagcc tggacctggc gagtcacgcc ataacgcgtt attcacccgg tctttcaggg
     4441 catccgtctg aagaagccag agcggcacat cacctgcggc ctgcgcccgg cggcccgttc
     4501 gtccggtgtt atcagggaat gtacggctga tcagttttgc gcgccggatg ctgtcaccct
     4561 taaacaggta aatacgttta ccaaggccat cacgacggca acgacgccag aatttatagg
     4621 cattatcagt gaccccgtct tcaccgccgg agtccaccgc cattgccatc agtcgcattt
     4681 gttgagaagg atcggaggcc agcggccagc ttttatgaaa aacatccgtc agcaggacat
     4741 cccagtcttc cggatagctg gccggatcaa ttcgctggct ctccccgtcg cagtcactgc
     4801 gcaatgactg cgtgatgttg taacgatcaa taatccagcg ttcgccacga ctgccatagc
     4861 ccgttacctg aaccacaaaa cggcgatgac gtcccgcctg cacatccact gtcgccacaa
     4921 ggaaattaac gccatccggc acactgcggg aaggaactgg ctctgcccgc tgctcaagca
     4981 gttcactttt tcgttgctcc atgctggcac gaggaagata aggcaatccc cagtcggtgt
     5041 tgataaccgc cctgagtgtt tcttcgcttc ctgtcgcttc atactcctgt tctgcagtca
     5101 gtaatttgta aaccagttgc gcccaggtct gatacgcagc agctggccct tccatccaag
     5161 aagaagtaaa taaaaggtaa ctaaatgatt ataaatagtt atttagttat actacctcct
     5221 acatagtcca agatttagta cctgcttaaa cgataaaaaa agaaacaaaa gcagcaatat
     5281 taattaaaag ctgaatgatg atgggggtac gcttgtgtct gattccggta acgcggtgga
     5341 ttaatcccag tatttcggct tctgtcattg tgcccccatc gcgctggtga agaaaacaag
     5401 ctcagatttt ttgtaaagaa tctgtcacgc taaaagatgt cgaacaaaaa ataaccttct
     5461 tcatcatctt ttt
//
LOCUS       DAZGXZ010000238         5359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000238 DAZGXZ010000000
VERSION     DAZGXZ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.239"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHKJF_005162"
     CDS             complement(<1..200)
                     /locus_tag="ACHKJF_005162"
                     /inference="COORDINATES: protein motif:HMM:NF020332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase domain-containing protein"
                     /protein_id="HFP0952358.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADL"
     gene            complement(200..2398)
                     /gene="traD"
                     /locus_tag="ACHKJF_005163"
     CDS             complement(200..2398)
                     /gene="traD"
                     /locus_tag="ACHKJF_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0952359.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKEVA
                     RMLKKDGKASDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGIS
                     ESGEVVDMTAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            complement(2449..3186)
                     /locus_tag="ACHKJF_005164"
     CDS             complement(2449..3186)
                     /locus_tag="ACHKJF_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952360.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPKKLAEVSREIIVASAQEIVWTPGSSRIDGHQEENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGYHHRHTGYTWSVSVN"
     gene            complement(3389..4120)
                     /gene="traT"
                     /locus_tag="ACHKJF_005165"
     CDS             complement(3389..4120)
                     /gene="traT"
                     /locus_tag="ACHKJF_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP0952361.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNTGSAGATLGVGLATGLAGMAADALVE
                     DINYTMITDVQIAERSKVAVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            complement(4134..4643)
                     /gene="traS"
                     /locus_tag="ACHKJF_005166"
     CDS             complement(4134..4643)
                     /gene="traS"
                     /locus_tag="ACHKJF_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898910.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFP0952362.1"
                     /translation="MITHYDIKMEMQKLKEVLSVEGVNIPSLLQVIKPGTYVFLWVLL
                     WPTFLRLVSVKSDVRDVGFDICASGMMGFLLFVAITNGMMLYLAIPDSFRKDSKIINF
                     MYSKSKTYILLFLIVFSMVSFMHSILYVFALMITFILFFLVYTIDINRYNLSAIASVI
                     GLFKKESVS"
     gene            complement(4640..>5359)
                     /locus_tag="ACHKJF_005167"
     CDS             complement(4640..>5359)
                     /locus_tag="ACHKJF_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HFP0952363.1"
                     /translation="SHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASR
                     TESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQVQP
                     GVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRNDVKHQVD
                     NMVTEYKGNIGDTQNSIHGEENIVDRQYSELKNNHKQEEIQQNNRYNEENKRQKLMPT
                     PSEDALKQMVDDKKERLKGPL"
BASE COUNT         1346 a         1429 c         1148 g         1436 t
ORIGIN      
        1 cttagatccg ctccgtccgg cagccttccc tccagaagac gggtaaaaac atccttatcg
       61 acactgccct gcagtcccag ctgttcagcc cccctgccgg cccagcgttc tcccatgctg
      121 cccagtacat agtaattatc cttgtcggta taatagttac cggcacttcc ggccgatctg
      181 acctgcgcga tactcatcat cagaaatcat ctcccggctc aacatcctcc ccacgctccc
      241 ggtgcacatt aatgttcacc tcttcacgac gctgcatctg ctgctggatg tccggatgat
      301 tttcctgttg ccatgcctca taagcggtca tatccaccac ttcaccggat tcactgatcc
      361 cgggtggcag ttgctgttcc atctcttctt ccggtttcat tttcagctcc tgctcgatcc
      421 cccctgccgg aacattcaca cctgaatctg acttcttatc gttgatggca ggagacaccg
      481 gctgttgtgg ctgttgtggc tgttgtggct gttgtggctg ttgtggctgt tgtggctgtt
      541 cagcctgagt cacatcttct ccggaaacaa cctccggcac atccggttcg aagaggctgg
      601 ccatctgacg accttctgct tcccttgcgg caagtacggc actcaggcgg ttctccattt
      661 ccgggttgat gtcacgcgga ataaactccg gagcgacctt cggtcgtgcc tgatatttca
      721 gagagagttt gactgccgga tacggtccgg gcagagtgac atagcaggtc agatccggca
      781 gggactgaat gtcggaataa ctgaccagcg tctgacgctc catatcctta ccggtcgata
      841 ccccgtcacg taccgggtca gcaccgtagg aatactgctc gctggctttc aggtgctctt
      901 tctcaccaat ttcaccggca gcgaactctg caatcttatg gctgggagaa cggaaaaagg
      961 cacgggtgtt catgacgtca aacagcgtgg ccgccgcttt ctcaccgtag atatcttcca
     1021 gctgggcata ggactggata ccaaacacat aacagccacc gaacttacgg gcttccggca
     1081 ggatctccac caggtccggc agtttgtgta acgtgggtaa ctcatcacag aaaaaccaca
     1141 cacgacggtt acggttttct cccattgcca gcagaccacg aatggcaatg gacagccaca
     1201 tggagatcac cggcttcagg gaggcatggg tgtcggcatt cgacgagata aacagccagc
     1261 cgtttttctg atcttcccgg acaccccgca tccagtcacg gatggtaaaa gactcaccgt
     1321 tatgctcaat cccctgcagg taacggatgg ctttcacgta gttggtcagc acagcacgga
     1381 tggaaatcgc cgttttctca attttctctt ccaccaggtt ggccgccgga gaattacgca
     1441 ggaaggtacg cagtttttca attttgatgg aaagcagtgt gtccaccagt ttgctgtaac
     1501 tgcggttggg gtcattacgc atcaggtacg ctgcttccgc aaaaatggtt cgtcctgacc
     1561 cctgccagaa cgggtcctct tttgtaccca tcgggatcag agtatttgcc gtattatcaa
     1621 aatccggctg tgtcaggcac tccttccaca aatcccatgc ggcacagcgg gcatccagcg
     1681 gattcaggat cttatcgatg gagggatcat agtaactttt aacaaattcc cccgaacggt
     1741 cataaatcac caccatatcg ccacgctgac gggcgtagtt ggccagacgt cggataactt
     1801 ccgacttacc ggcccccacc gtgccgtgca gacagaagtt ctggatttcg gaatcccgga
     1861 taatcggcag gtcgccaatc cggatatccg aggctttacc gtcttttttc agcatccggg
     1921 caacctcttt cgggttctcc gtcagctgac gaccacctgt gacttcattt tcgctctgtt
     1981 gtttaccctg acgccccaga atccaggaga caacaaagaa ggtaatcagg cagataacca
     2041 gggccacgac agacgccaga acgaatgcag accatagctg ctcaccgcac cagatcatat
     2101 atttatcatg cagtacctga gccgcattca tccggaaggt tttgccgtaa tactggatct
     2161 catagaccgg ctgggatttt attaaatccc gcatgccttc cagcgtggta caccaccagt
     2221 aaatgcagcc attcacaaac gtctgccagc ttatttttac ccataagacc agaccaatga
     2281 ggatccagaa aaaaataaac aggcagtaaa gcatgatatt ggcgatctgg ctgaacatgc
     2341 ggatacgcat ggacgcaatc tgaccgccct gggtcatatc ctttgcgtta aaactcacga
     2401 tgaaactccg ggaaaatctg gaacagagca gaaaagaatg atgtggaatt aattcacaga
     2461 aacagaccag gtgtaccctg tatgccggtg gtgatacccg cctgcagtcc tgccggaaga
     2521 gaccttctct gtaacaatgt cagtcaggcg caatacttca gaccagtcac tcccgtgcgg
     2581 aaactggatc tgaataaaaa ggcgtacccg ttcacggtga tccagaactg tatccggttc
     2641 ataaaaacgg ccactgtcag gagaaacaca ggacgtttta ttgatgagat catacttatc
     2701 ggcaggagac tgcgatgaaa tcgcccaggt aatattctcc tcctgatggc catcaatacg
     2761 actgctcccc ggtgtccaga caatttcctg cgcactcgcc acgatgatct ccctggagac
     2821 ttctgccagc tttttgggtg tacagaatac cggtgccagc acacacaggg acaacaaatt
     2881 acagctgacc gcctccggtt taagcaaatt gctcccggat ttttcaggaa aagatatttt
     2941 tgtcagtatt tcagttaatt ccttatgcct gagagaatta ataaagtaca tgataagcat
     3001 catgagaaac aatataacaa taagccctcc gataaccact aaaaggtcag tatcaatatt
     3061 attgctgtac agatagtctt tagtggtcac tgtattatcc tgtatcaccg gggggtattc
     3121 agttacaggc ttctcagtaa attgattatt ctcaccaaca tattcagcga tattgtctga
     3181 tgtcataatg actttctccg gaagatcccg gcttcgccgg caacacttca cggtcgcaca
     3241 aagaatttgc acaaccgctc agtgttttca tatttatttc ggaaacgccg ggaggtgaca
     3301 aaatttattt tgttacatcc cggggttgga caacgagcaa cgcgaggcgt cagcaaggag
     3361 gccggtcaga tcagcctcct gacgaggttc agagaatatt tgcgattgat ttggccagct
     3421 ggtcttcgag aacaggcttc gcctcttcaa atttcaggtt aaccttgttc gcatttgaga
     3481 ccacacgggt ctggtattta tgctggttac ccgtttcagt actggtctga attttcgcac
     3541 cggatgttcc ctgacgcagc gcagcaacgt tatccgttgt gacggccact ttactacgtt
     3601 ctgcaatctg cacatcggtg atcatcgtgt agttaatatc ttcaacaagc gcatcagctg
     3661 ccataccagc aagaccggta gccagtccca cccccagcgt tgcgccggca gaacctgtgt
     3721 tatatccggt aataccggca cctaacgctg caccaactgc ggccccttca tagccccggt
     3781 tcagccagcc ctgagactcc cgcagatcca tcttatcggc tttcagcaca ttcgcctgga
     3841 tccagtagta ggctttatcc ggagaagtca ccacctgata cccttttgct ttcacagcat
     3901 cagcaatttt gccctgcagc ccactcatgt ctttatcaga cgtgtttttg atctgcagaa
     3961 ataccgtgcg ttcactggcg ggttcaagcc agatggtctc actcatctga gtcttcacct
     4021 caaggttacg cttcttgatt gctgtgctca tcgcaccaca ccctgaaagg gccagagtgg
     4081 aactgaccag tgcaaccatc attaattttt ttgttttcat ccaaaatatc cttttaacta
     4141 acagactctt ttttgaacag tcctattact gaggctatag ctgagagatt ataacgattt
     4201 atatctatgg tatacactaa gaaaaataat atgaatgtaa tcattagtgc aaaaacatat
     4261 aagatagaat gcataaacga aaccatagaa aaaacaatca aaaacaacaa aatatatgtc
     4321 ttactcttag aatacataaa atttattatt tttgaatcct ttctgaaact atcaggaatc
     4381 gcaaggtata acatcattcc atttgtaata gccacaaaaa gcaggaaccc catcattcca
     4441 ctagcacata tatcaaagcc aacatccctg acatcacttt taaccgatac taaacgcaga
     4501 aaagttggcc ataataaaac ccataaaaac acataagttc ccggctttat gacttgtaat
     4561 aatgatggga tattaacacc ctctacagag agtacctcct ttagtttttg catctccatt
     4621 tttatatcat agtgagtgat cataatggcc ccttcaatcg ttctttctta tcatccacca
     4681 tctgttttaa cgcatcttca gacggtgttg gcattaattt ctgtcgttta ttttcttcat
     4741 tatatctgtt gttttgttga atttcttcct gtttgtgatt attttttaac tctgaatatt
     4801 gtctgtctac aatattttct tcgccatgaa tactattctg agtatctccg atattccctt
     4861 tatattctgt gaccatatta tcaacctgat gttttacatc attacggata ttactgtcct
     4921 gcgttctttg atcaataatg gcctgatgcc cctgatggtc agcaataata tcctggctgc
     4981 caccacccga aggtacgctc tccattcctt taccaatatc cccacgggat tcacgccagg
     5041 cattatcaac accaggctgc acctgttcct gaacaaaaga ccatgccatc gcccgacggc
     5101 gttctgcaat ctccggtgaa ctggtgtttg tcagaatagc ttctgcatcc tgcggcgcgt
     5161 gtttcatcac atactgtgca aactgttgcg acagatcttc actcatctgc ccactcatgc
     5221 tttcagtacg ggatgccatt tcagcatatt catggctgcg ggtcatattc gtcgtgtact
     5281 ggtcgtaact ctgtttcgct gagttcaggg ctgcagacaa ctggtccacg cgggaatcag
     5341 cattattgtc agtatggct
//
LOCUS       DAZGXZ010000239         5275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000239 DAZGXZ010000000
VERSION     DAZGXZ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.240"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            61..480
                     /locus_tag="ACHKJF_005168"
     CDS             61..480
                     /locus_tag="ACHKJF_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFP0952364.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQVRDRYREALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPLVQSDAAVFGRNQKGFI"
     gene            477..1037
                     /locus_tag="ACHKJF_005169"
     CDS             477..1037
                     /locus_tag="ACHKJF_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFP0952365.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDGVLRSMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQADSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVGP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            1038..1283
                     /locus_tag="ACHKJF_005170"
     CDS             1038..1283
                     /locus_tag="ACHKJF_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFP0952366.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKPFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            1280..2782
                     /locus_tag="ACHKJF_005171"
     CDS             1280..2782
                     /locus_tag="ACHKJF_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0952367.1"
                     /translation="MINFDGIGNDRRIPLIEVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGAGVLDVPVRITRASQATELWGRGSMIALMVAEFIAINPDAELYAIAQGNGT
                     GQATAAAMNLTGTVTRDGIVYACVGGRRYTLPAPKGKKGKELTDELAALINADPDAPF
                     TASSGAGSGDNGAGLKGSLGITARFTGECSVHDVRLNYYDGEATPEGIQVAIAYPKQK
                     AANPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDVVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSWLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNASDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            2791..3156
                     /locus_tag="ACHKJF_005172"
     CDS             2791..3156
                     /locus_tag="ACHKJF_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFP0952368.1"
                     /translation="MSGKQYQGTATIRVNGQEYETLEGATFSPSGFEREVVKGAKVYG
                     YRQKPREATLDCKFPAGGEGSPAADEINNWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGEISVKFASASSTRVQ"
     gene            3171..3647
                     /locus_tag="ACHKJF_005173"
     CDS             3171..3647
                     /locus_tag="ACHKJF_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP0952369.1"
                     /translation="MTTRKKKTAVSEAAVMEAIREALEGADPRTAGLTEQLAKGYVDL
                     LDGLPFGETREYRVTFRELTAKDSIDAEAEAERVVETNNGPMLIASPSLRGVALLRRQ
                     IAAVGDIEGPLSPRQIGQLSERDLSRLMAAVSLLDTALAGKLAADRGRSGAVSGSD"
     gene            3774..>5275
                     /locus_tag="ACHKJF_005174"
     CDS             3774..>5275
                     /locus_tag="ACHKJF_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0952370.1"
                     /translation="MARNLRASLIVDLLGNISAKSRQWSQELGAFSRSGQAGLGGLGN
                     AARRAGQETDVLGSRMQRTLAGVRGSIRHVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNRGFIRPAAEMETYMIRLNSLYKGDRAKTDDVRRWAIQNAKETTWGLAGVMQ
                     EYTSSLGFGMSDREARNFVTMLQDQGAVGGWSLSDAQGASLQLKQMYARGSIQAADAN
                     ILATYGINAYRVLADRLGVDQKVVRKLGEKGLLGPDSIRLLFQTLAEQARGAQKDAMN
                     SWSGLTAMMGDVWDQFARDVMDSGPFEKLKGNLRGFLSWVDSAKSDGSYHSLAESTAS
                     AMNQGFEYARDAVTGFYQAIRKVRDTLQALRDAGYGDALDRIGQGAQTAAKYLLYMYL
                     ATRALKMARAVGTGIIRPGAALLGYGLSAAAFLTSPFRRSPPAGTPPGTSQGRGQRFI
                     NFLTGVNPAAVQPVLVTNWPTGGLSGTGATDTSSGGRQGRGRRKRGPGRG"
BASE COUNT         1183 a         1370 c         1624 g         1098 t
ORIGIN      
        1 acaggatgct ccccggaaaa agaccggtaa caaagcgaaa caggcgaaaa aggcccgcgc
       61 atgaattacg ccactgaaac cgatatgcgg gcgcgttacc gcgaggattt gctcaggccg
      121 ttactggctg tgccccgttc ggatgagccg gacacgcgca agctgaaccg ggcgctgaca
      181 gatgcgtcag cccttatcga cagctatctg tccgcccgtt acacgttgcc gctggaggtt
      241 attccggccg ttcttgttca gcactgttgc gcgattgcct tttattacct gtgcgatcag
      301 cgagcctccg atcaggtgcg tgaccgttac cgtgaagcgc tggcctggct gaaagatgtc
      361 atgaacggca atgtgccggt cggtgtggat acgaacggtg cggcccctga atccggggat
      421 ttaccactgg ttcagtctga tgcagcggta ttcgggcgca accagaaggg cttcatatga
      481 ttacggaaac cgaacaggca tacatcgccc gtatccgtga gtatttcggg aatgaacttg
      541 tgtctgttga cacgcatccc ggcgactgga gcgacggcgt actgcgcagc atgctgatta
      601 acgccccggc gatctacgtt gcctggctgg gtgccggtga aggccgtacc cgtggtcgcc
      661 tggtcagtca ctgggtgttc tacgtcatcg gcgacatgct caacgggcgt gaggccagcc
      721 gtcccggact gtatcagatt gtggcccggc tgattgccgt gcttaacggc ttcagaacag
      781 aaaaaacctc accgctttac tttgaaaagg ctgtcaacgg ttacaccgaa acccaggcgg
      841 acagtggtgc ggtgatgtat gcgctttatt tctcctgcga ggaaatgatc gcaccgctga
      901 ccgatatcag ctcgctggac gacttcctgc gtcattacga aacctttgtg gggccgcccg
      961 gtacgccgcc gtttgaggca catattgatt tgccggggaa cacgacaacg gcggtcgaac
     1021 cgccggagga actgtaaatg aaaaccatct ttatcaaacc cgcgccggga cgcctgattc
     1081 gtgatcccga cacaatgcgc ccgctggcac aggaggggga ggaaaagccc tttacgccgt
     1141 tctggtgtcg gcgtcttgat gacggcgatg tcattcaggc tgaaaaagcc gctgaagagg
     1201 ctccggctgt atctgcggat gcaacgactg cgacagaaaa accggtcgcc caaccggcat
     1261 ccgataagga gaaaccgcaa tgattaattt tgacgggatc ggtaatgacc gccgtatacc
     1321 gctgattgag gttgagttta ataactccat ggctgtcacc ggcactccgg cacagcgtca
     1381 gtgtgtgctg ctgtttggtc aggcgagaat gaaagagaat gccgtggacg gtgccggtgt
     1441 tctggatgtt ccggtcagga tcacccgtgc gtctcaggca acagaactct gggggcgcgg
     1501 ctccatgatt gccctgatgg tggcggaatt tatcgccatc aatcctgatg ctgaactgta
     1561 tgccattgct caggggaacg gcaccggaca ggcaacggct gcggccatga atctgaccgg
     1621 aacggtcacc cgtgacggga ttgtttatgc ctgcgtgggg gggcggcgtt atacactccc
     1681 ggcaccaaaa ggtaagaaag gcaaagaact gaccgacgag ctggccgcgc tgatcaatgc
     1741 cgatcctgac gcgcccttca cggcctcctc tggtgcagga agtggtgata atggcgcagg
     1801 tctgaaaggg tctctgggga tcaccgcccg ctttaccggt gaatgctccg ttcatgatgt
     1861 gcgcctgaac tattacgacg gggaagcaac cccggaagga atacaggtcg ctatagccta
     1921 tccgaaacaa aaagcggcta acccggatat cacccgaagc gtggcgggta tgggcgatcg
     1981 ccagtacaac tatgtcgtca tgccctataa ggatgatgca aacctgaaaa tcatcagtga
     2041 tgagcttctg aaacgctggg gaccggccaa aatgtctgac ggcgtgctgt ggctggcaca
     2101 taccggcacg tttggtgagg tgcaggcatt cggggcaaaa cgcaatgatt ttctggcgac
     2161 ctgcacatcc attcccaaag cccctgaacc ggattacctc tgggcagcgg cggtctgtgc
     2221 cacctgtgca ccctctctgg gagcagatcc tgcgcgtccg cttcagacgc tggcgcttcc
     2281 gtcacgcatg gccccggctc cggccgatcg tctgacgcgt gaagagcgta acagtctgct
     2341 gtggggaggg attgccacgg tcagtgtggc tgccggtgat gtggttcaga tcgaacgtca
     2401 ggtgaccatg tatcgccaga acgtgtacgg cgaaagtgat cccagctatc tggatgtgga
     2461 aaccatctac accctctcct ggctgcgtta ttccctgcgt acttttatca cacaacgttt
     2521 tccgcgccat aaactggctg atgacgggac gcccgttcgt gccggtcaga atatcgtgac
     2581 gccggagatc atgaagcttc agttaatttc gctgggtgaa gagtgggtgg aactcgggct
     2641 ggtcgaaaat ctggacacct ttaaaaagaa tctgcttgtg gagcgtaatg cgtcagatcg
     2701 aaaccgtctt gacgtgttat gcacgccgga tctggttaac cagttccgct tcctggcagc
     2761 acagatccgt ttcattttgt gaggtaaggc atgagcggaa aacagtatca gggtacggcc
     2821 actatccgtg tgaacggtca ggaatacgag acgcttgagg gggctacatt ttctccgtct
     2881 ggttttgaac gtgaagtggt gaaaggcgcg aaagtctatg gttaccgcca gaaaccacgt
     2941 gaagcgacgc tggactgcaa atttccggca ggcggagaag gttcaccagc cgccgatgaa
     3001 atcaacaact ggactgcggt cactatcgag tttgtggcgg ataccggcga agtccacatg
     3061 atgacgaagg cctggagcag tgaaccggcc tcgcttgacg gtggcgggga gatctccgtg
     3121 aagttcgcca gcgcctccag tacccgtgtt cagtgatcag gaaaaataaa atgaccacac
     3181 gtaagaaaaa aacggcagtt tccgaggcag ccgtgatgga ggcaatccgg gaagcacttg
     3241 agggggctga tccacgcact gccgggctga cagagcaact ggcaaaggga tatgtggatc
     3301 tgcttgatgg tctgccgttt ggtgagaccc gtgaatatcg tgtcacgttc cgggaactga
     3361 cagcgaaaga cagtattgac gcagaagcag aagccgagcg cgtggtggag acaaacaatg
     3421 gcccgatgct gatagcgtct ccgtcgttac gcggtgttgc actgctgcgc cgtcagattg
     3481 ccgcagtggg agacattgaa ggtccgttgt caccccgtca gattggacag ttaagcgagc
     3541 gcgatctctc ccgcctgatg gcagcggtca gcctgctgga taccgcgctt gccggaaagc
     3601 tggcagctga ccgggggcga tcaggcgcag tgtcgggatc agattgaaga agcggcaatt
     3661 atcctgggga tggtgacaaa aagcggtccg gaatgggcgc ttaacctccc cctgtcgcaa
     3721 ctttaccggc actgccgaca gacagaaaaa atcatcagaa cgaagcagta agtatggccc
     3781 gaaatctcag agcatctctg atagttgatt tgctcggcaa tatctcagcc aaatcccgcc
     3841 agtggtcaca ggagctgggg gcattctcac gctccggcca ggcggggctg ggtggcctgg
     3901 gaaatgctgc ccgccgtgcc gggcaggaaa cagatgtact cggaagcagg atgcagcgca
     3961 cgctggccgg ggtgcggggc agtatccgcc atgtcacttc tgattttgac cgtcttcagg
     4021 gcagtattac cggcaccatc gggcggatca gcaatcttta cggaatgctg gccggtggtg
     4081 ctgctgtata tggctttaac cggggattta ttcgcccggc agcggagatg gaaacctata
     4141 tgattcgcct gaactccctt tacaaagggg atcgggcgaa aactgatgat gtccgcaggt
     4201 gggcaataca gaatgcgaag gaaaccacct gggggctggc tggcgtcatg caggaatata
     4261 cgtccagtct gggctttggt atgagtgaca gggaggcccg aaattttgtc accatgcttc
     4321 aggatcaggg tgccgtgggc ggctggtcac tgtccgatgc acagggggca tccctgcaac
     4381 tgaaacagat gtatgcaaga ggcagtattc aggcggcgga tgcaaatatt cttgccacct
     4441 acggcattaa tgcctaccgc gtgctggctg atcgtctggg tgtggaccag aaagtggtca
     4501 gaaaacttgg tgaaaaaggg ctgcttgggc cggacagtat tcgcctgtta tttcagacgc
     4561 tggcggaaca ggccagggga gcacaaaagg acgcaatgaa ttcctggagt ggcctgacgg
     4621 ccatgatggg tgacgtctgg gatcagtttg cccgggatgt gatggacagt gggccatttg
     4681 aaaaactgaa gggtaatctc aggggatttc tttcatgggt tgacagtgcc aaatctgatg
     4741 gcagttacca ttccctggcg gaaagcacgg catcagccat gaaccagggc tttgagtatg
     4801 cccgtgatgc agtgacaggg ttttatcagg cgatcagaaa ggtacgcgac acgcttcagg
     4861 ctctgcggga tgcgggctat ggtgatgcgc tggatcgcat tggtcagggg gcacagaccg
     4921 ccgcgaaata cctgctgtac atgtatctcg ccacccgtgc cctgaaaatg gcgcgggctg
     4981 tcggaacggg gattatacgt ccgggtgctg cattgctggg atacggatta tcagcggcgg
     5041 ccttcctgac ttcacctttc cggcgctctc ctccggccgg aacaccacca ggcacatctc
     5101 agggacgagg gcaacgtttt ataaactttc ttaccggggt aaatccggca gccgttcagc
     5161 cagtacttgt aacaaactgg cccacaggag gactgtccgg caccggcgcg accgatactt
     5221 cctcaggcgg acgtcagggg cggggccgca ggaaacgcgg accggggcgc ggaag
//
LOCUS       DAZGXZ010000240         5257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000240 DAZGXZ010000000
VERSION     DAZGXZ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.241"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHKJF_005175"
     CDS             complement(145..627)
                     /gene="ybiA"
                     /locus_tag="ACHKJF_005175"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFP0952371.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRERSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            747..2897
                     /gene="dinG"
                     /locus_tag="ACHKJF_005176"
     CDS             747..2897
                     /gene="dinG"
                     /locus_tag="ACHKJF_005176"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP0952372.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHKJF_005177"
     CDS             2925..3887
                     /gene="ybiB"
                     /locus_tag="ACHKJF_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP0952373.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHKJF_005178"
     CDS             4028..5113
                     /gene="hcxB"
                     /locus_tag="ACHKJF_005178"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP0952374.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHKJF_005179"
     CDS             5180..5239
                     /gene="ybiE"
                     /locus_tag="ACHKJF_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP0952375.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
BASE COUNT         1238 a         1327 c         1484 g         1208 t
ORIGIN      
        1 tctgcaatat attgaatttg catgcttttg taggccggat aaggcgttta cgccgcatcc
       61 ggcatttcac aacaagcact tgtcagcaat ttgagaacac gggaaaaaat ttatctgttt
      121 taccgcccat aagccaccga gacgttactt ctctatagcc aattgttcgc gcaactccat
      181 taaaaggtag cccagtcgat tcttgccctt accattacca ccgtctcccc agtaagcatc
      241 gttttccgta tgctcaacca gttttgcggg cgcggttgcc agcaagagcg cacgcagttc
      301 tggatgctgt tcgaatttag cgcgaagtgc ttttcgcatc acctgttctt tgaccgactc
      361 ccagttttta cgcagaggct tagaacgctc gcgccccatg cgtgcggcga ccatagggga
      421 agaaacccga cggatctctt cgcggtattt ttcatcaagg aatttttgtg cctgaaaata
      481 gtgttctgag gtgggccagg ttttcccgtc aaccttgatg ggccaggcgg caaagttaga
      541 aaaatcaccg tagtcatcgc tggtgctgta gaaatttatg atggtgtctt gcatgacgtg
      601 ttggatcctt tgtgctcgaa cgggcattaa accgcattat gttggtggtt attgcgagcc
      661 gctttccaga aacagaaaaa ccattacccc tgaaaaccga aaaatgccac aatattggct
      721 gtttatacag tatttcaggt tttctcatgg cattaaccgc cgcgcttaaa gcgcaaattg
      781 ccgcctggta taaggcgctt caggaacaga tccccgactt tattccccgt gcgccgcagc
      841 ggcagatgat tgcggacgtc gccaaaacgc tggccggaga agaagggcgg catctggcga
      901 ttgaagcccc caccggcgtt gggaaaacgc tctcctattt gatccccggc atcgccattg
      961 cccgcgaaga gcaaaaaacg ctggtggtga gtaccgccaa cgtggcgttg caggatcaga
     1021 tttacagcaa agatttaccg ctgctgaaaa agatcattcc cgatcttaaa ttcactgccg
     1081 cttttgggcg tgggcgctac gtttgcccgc gtaatctgac ggcgctcgcc agcactgagc
     1141 ccacgcaaca ggatctgctg gcgtttcttg acgacgaact gacgccaaat aatcaggaag
     1201 agcaaaaacg ttgtgcgaag ctgaagggcg atctcgacac ttataaatgg gatggtctgc
     1261 gcgatcatac tgatatcgct atagatgacg atctctggcg tcgtttaagt accgacaaag
     1321 ccagctgcct caaccgcaac tgttactact atcgtgaatg cccgtttttt gttgctcgtc
     1381 gggagattca ggaagcggaa gtggtggtgg caaaccatgc gctggtgatg gcggcgatgg
     1441 aaagcgaagc cgtactacct gacccgaaaa atctgctgct ggtgctggac gaaggtcatc
     1501 acctgccaga tgtggcccgg gatgcgctgg agatgagtgc cgaaatcacc gcgccctggt
     1561 atcgactaca gctggacttg ttcacgaaac tggtcgctac ctgcatggag cagtttcgcc
     1621 cgaagaccat cccaccgctg gcgatccctg aacgtttgaa tgcgcattgt gaagagttgt
     1681 atgagcttat cgcctcgtta aacaacattc tcaatctcta catgcctgcc gggcaggagg
     1741 cagagcaccg ttttgcgatg ggcgaactgc ctgatgaagt gctggagatc tgccagcggc
     1801 tggcaaaact caccgagatg ctgcgtggtc tggcggagtt atttcttaac gatttaagtg
     1861 agaaaaccgg cagccacgat attgtgcgtc tgcatcggtt gattttgcag atgaaccgcg
     1921 cgttggggat gttcgaggcg caaagcaaac tctggcggct ggcgtctctg gcgcaatctt
     1981 ccggtgcgcc ggtgaccaaa tgggcgacgc gggaagagcg cgaagggcag ctgcatctct
     2041 ggtttcactg cgtggggata cgtgtcagcg atcagctgga aaggctgctg tggcgcagta
     2101 ttccgcacat tattgttacc tccgcaacct tgcgttcgct gaatagtttt tcgcgtttgc
     2161 aggagatgag tggtctgaaa gagaaagcgg gcgaccgttt tgtggcgctg gattccccct
     2221 ttaaccactg cgaacagggc aaaattgtta ttccccggat gcgcgttgag ccttccatcg
     2281 acaacgaaga gcagcatatt gccgaaatgg cggccttttt ccgtaagcag gtggagagca
     2341 aaaaacatct cggtatgttg gtactgtttg ccagcggacg ggcgatgcag cgctttctcg
     2401 actatgtgac ggatttacgt ctgatgctgc tggtgcaggg cgatcagccg cgttatcgct
     2461 tagttgaact gcaccgcaaa cgcgttgcca acggtgagca tagtgtgctg gtgggcttac
     2521 agtcatttgc cgaagggctt gatttgaaag gtgatctgct cagccaggtg catatccaca
     2581 aaatcgcttt tccgcccatc gacagcccgg tggtgatcac cgaaggggaa tggctgaaaa
     2641 gcctcaaccg ctatccgttt gaggtgcaaa gcctgccgag cgcctcgttt aacctgattc
     2701 agcaggttgg gcgactgatt cgaagccacg gttgctgggg cgaagtggtt atctacgaca
     2761 aacgcttgct gaccaaaaat tatggcaagc gactactgga tgcattaccg gtatttccga
     2821 tagagcaacc ggaagtccct gaaggtatag ttaaaaagaa agaaaaaacg aaatccccac
     2881 gccgtcggcg gcgttaatga tgtgagtcag gtaaggagtc gtaaatggac tatcgcaaaa
     2941 tcattaaaga gatcgggcgc gggaaaaacc acgcgcgcga tttagaccgg gatactgcgc
     3001 gcggtctgta tgctcatatg ctcaatggtg aagtccctga ccttgagttg ggcggcgtat
     3061 tgattgcgct gcgtatcaaa ggagaagggg aagcagagat gctcggcttt tacgaagcca
     3121 tgcaaaatca caccatcaag ctgacgccgc cagcaggcaa gccgatgccg attgtcatcc
     3181 ccagttacaa cggcgcgcgt aaacaggcca acctgacgcc gttgttggcg attctcctgc
     3241 acaaactcgg ttttcctgtg gtggttcacg gggttagcga agatccaacc cgcgtgctga
     3301 ctgaaaccat ttttgaattg atgggtatta cgccaacgtt gcacggcggg caggcgcagg
     3361 cgaagctcga cgaacatcaa ccggtgttta tgccagtcgg cgcgttttgc ccgccgctgg
     3421 aaaaacaact ggcgatgcgc tggcggatgg gcgtgcgtaa cagtgcacat accctggcga
     3481 aactggcgac gccatttgcc gaaggtgagg cgctgcgtct ttccagcgtt tcgcatccgg
     3541 aatacattgg acgcgtcgcg aagttcttta gcgatatcgg cgggcgggcg ctactaatgc
     3601 atggtactga aggtgaagtg tatgctaatc cgcagcgctg cccgcagatc aatctcattg
     3661 accgtgaagg gatgcgggtg ctgtatgaaa aacaggacac tgctggtagc gagttactgc
     3721 cacaagcaaa agatccggaa accacggcgc agtggattga gcgttgcctt gctggcagcg
     3781 aaccgattcc cgaatcgctg aaaatccaga tggcttgctg cctggtggct acgggtgaag
     3841 cggcaactat cagcgacggc ctggcgcgcg ttaatcaggc attttaattc ttttctcagc
     3901 cggatgagcc atgtcatccg gcttttaccc caccgcaata tgaaattcct gcatctttat
     3961 tgaccttccc acgcccggcg tgcagcataa aaatacaaca aacacataac ataaacagga
     4021 gttaaccatg gaaagtggtc atcgctttga tgctcagacg ctgcacagtt ttattcaggc
     4081 tgtatttcgt cagatgggta gcgaggaaca agaagcgaaa ttagttgccg atcatttaat
     4141 cgcggcaaac ctggcagggc atgattcaca tggtattggc atgatcccaa gctatgtacg
     4201 ctcctggagt caggggcacc tgcaaattaa ccatcatgcc aaaaccgtta aagaggcggg
     4261 ggcggcggtc acgctcgatg gcgatcgcgc atttggtcag gtcgcggcac atgaagcgat
     4321 ggcgctgggg attgagaaag cgcatcagca cggtattgcc gccgtggcgc tacataactc
     4381 gcatcatatc ggccgtatcg gttactgggc ggagcagtgt gcagcggcgg ggtttgtctc
     4441 tatccacttt gttagcgtgg tcggtattcc aatggtcgcg ccgttccacg gtcgcgacag
     4501 ccgctttggc accaatccgt tctgtgtggt tttccctcgt aaagataatt tcccgctgtt
     4561 gcttgattac gccaccagcg ccattgcatt tggcaaaacc cgcgtcgcct ggcataaagg
     4621 cgtccccgtg ccgccaggtt gcctgattga cgttaacggc gtgccgacga ccaatccggc
     4681 ggtaatgcag gagtcgccgt tgggttcgct gttgaccttt gccgaacata aaggctacgc
     4741 ccttgcagcg atgtgtgaaa ttcttggcgg ggcgctttcc ggcggtaaaa cgacgcatca
     4801 ggaaacgtta caaaccagtc ccgatgccat tcttaactgc atgaccacta tcatcatcaa
     4861 cccggaactg ttcggcgcgc cggattgtaa cgcgcagacc gaagcctttg ccgagtgggt
     4921 gaaagcctcg ccgcatgatg atgataagcc gattttgcta ccgggcgagt gggaagtgaa
     4981 cacgcgtcgc gaacggcaga agcaggggat tccactggat gcgggaagct ggcaggccat
     5041 ttgtgatgca gcgcggcaga ttggtatgcc ggaagagacg ttgcaggctt tctgtcagca
     5101 gttagccagc taaaaaaaag cccgtccagt ggcggacggg caaacaaggg taacatagga
     5161 tcaatgaggg ttagagcata tgcgtctgtc ggcaaacaga cagggaaata cttgtgctgg
     5221 acgtagcgta aacgcctgat ccgtccaacg gttcgga
//
LOCUS       DAZGXZ010000241         5107 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000241 DAZGXZ010000000
VERSION     DAZGXZ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.242"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..717
                     /locus_tag="ACHKJF_005180"
     CDS             <1..717
                     /locus_tag="ACHKJF_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HFP0952376.1"
                     /translation="SHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEYAEMASR
                     TESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFVQEQVQP
                     GVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQGIIEQRTQDSNIRNDVKQQVD
                     NMVTEYRGNIGDTQNSIHGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSAQEGMPG
                     ADSPQELMNRAKEYQDKYKQ"
     gene            739..1218
                     /locus_tag="ACHKJF_005181"
     CDS             739..1218
                     /locus_tag="ACHKJF_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFP0952377.1"
                     /translation="MKNLAHVALITVIQLIACNLADWGGAETVFILCFIVLWQGLFIW
                     LFSQIRKKRHVSDEFKFSKGVWYILMPVSSLFSPLLSLMVFIIGSLYELRRVCGCVSV
                     REWMQSQVNEKTNEDLHLDFDNMDFHRTNPATGLPMLGIGVDIGGNLYGYSSKRYDE"
     gene            1267..1998
                     /gene="traT"
                     /locus_tag="ACHKJF_005182"
     CDS             1267..1998
                     /gene="traT"
                     /locus_tag="ACHKJF_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP0952378.1"
                     /translation="MKMKKLMIVTLVSSTLVLSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPSSERTVFLQIKNTSDKDMSGLQAKISDAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQTWLNSGYEGAIAGAALGTGITGYNSSSAGAALGVGIATGLIGMAADAMVE
                     DINYTMITDVQIAERTTSEVRTDNVAALRQGTSGAKVQTSTSTGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            2058..2318
                     /locus_tag="ACHKJF_005183"
     CDS             2058..2318
                     /locus_tag="ACHKJF_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952379.1"
                     /translation="MNRAESRLSQVLFLLGYDSEKYPEHVCRELLKELYRFADATGDS
                     ELAIACQRYLTALEPFSAYEIGVSATEYLPVSEYPTGRRRRK"
     gene            2486..3217
                     /locus_tag="ACHKJF_005184"
     CDS             2486..3217
                     /locus_tag="ACHKJF_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952380.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELAEILTKISFPEKSGSNPLKPEEGRC
                     NLLSLCVLAPVFCTPKKLAEVSRQIIVTSAQEIAWTPGSSRIDGEQEENITWSLSSQT
                     HADKYDLINKTSRVSPDSGHFYEPGTALDYRERVRLFIQIQFSHESDWSEILRLTDIV
                     LEKVSSGRSAGGYHHRYTGYTWFVL"
     gene            3273..>5107
                     /gene="traD"
                     /locus_tag="ACHKJF_005185"
     CDS             3273..>5107
                     /gene="traD"
                     /locus_tag="ACHKJF_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0952381.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIRSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVSTGKSEVIRRLANYARKRGDMVVI
                     YDRSCEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNVANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTYLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHSGESFTIRDWMRGVREDKQNGWLFISSNADTHA
                     SLKPVVSMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVLNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKEMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARA
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGE"
BASE COUNT         1364 a         1122 c         1326 g         1295 t
ORIGIN      
        1 agccatactg acaataatgc cgattccagg gtggaccagc tgtctgcagc cctgaactca
       61 gcgaaacaga gttacgacca gtacacgacg aatatgaccc gcagccatga atatgctgaa
      121 atggcctccc gtactgaaag catgagtggg cagatgagtg aagacctgtc acaacagttt
      181 gcacagtatg tgatgaaaca cgcgccgcag gatgcagaag ctattctgac aaacaccagt
      241 tcaccggaga ttgcagaacg tcgtcgggca atggcctggt cttttgttca ggaacaggta
      301 cagcctggtg ttgataacgc ctggcgtgaa tcccgtggcg atatcggtaa aggaatggag
      361 agcgtacctt cgggcggtgg cagccaggat attattgcag accatcaggg gcatcagggt
      421 attattgagc aaagaacgca ggacagtaat atccgtaacg atgtgaagca gcaggttgat
      481 aatatggtca cagaatatcg gggcaatatc ggagatactc agaacagcat ccacggcgag
      541 gaaaatattg taagagggca gtattctgaa ctgcaaaatc accataaaac agaggcatta
      601 tctcagaata ataaatataa tgaagaaaaa tcagctcagg aaggaatgcc cggtgctgac
      661 agcccacagg aacttatgaa cagggcaaaa gagtatcagg ataagtataa gcaataatgc
      721 tgggtttggg agatgataat gaaaaattta gctcatgttg ctctgataac tgtgatacag
      781 ctcattgcct gtaatctggc tgattggggg ggcgctgaaa cagtttttat cctgtgcttt
      841 attgtactat ggcagggatt gtttatatgg ttgttttctc aaatacgaaa aaaacggcat
      901 gtgtcggatg agtttaaatt cagcaaaggc gtctggtata tactgatgcc agtatcttcc
      961 ttattttccc cgttattaag cctgatggtt tttatcattg gttcactata cgaattaagg
     1021 cgagtttgtg gttgtgtaag tgtaagagaa tggatgcaat ctcaggtaaa tgaaaaaact
     1081 aatgaagatt tgcatctgga ttttgataat atggattttc atcgtactaa tcctgcaaca
     1141 gggttaccta tgttaggtat tggtgtggat atcggtggta atttatatgg ttatagttca
     1201 aaacgatatg atgagtgaat cttaatttgt atattatggt gttttattca atatgaagga
     1261 acattgatga aaatgaaaaa attgatgata gttaccctgg tcagttccac tctggtcctt
     1321 tcagggtgtg gtgcgatgag cacagcaatc aagaagcgta atcttgaagt caaaactcag
     1381 atgagtgaaa ccatctggct ggagccttcc agtgaacgca ccgttttcct gcaaatcaaa
     1441 aacacatctg acaaggatat gagcggattg caggcaaaaa tcagtgatgc cgttaaagcc
     1501 aaaggctacc aggttgtgac ctcacctgac aaggcatact actggatcca ggccaatgtc
     1561 ctgaaggcag acaaaatgga tttgcgggag tcccagacct ggctgaacag cggttatgaa
     1621 ggtgccattg ccggtgcagc attgggcacc ggcatcacag gatataattc cagctctgcc
     1681 ggtgcggcgc tgggtgtagg gattgccaca gggttgattg gtatggctgc cgatgcaatg
     1741 gtggaggata tcaactacac catgatcacg gatgtgcaga ttgcagagcg gacaacatct
     1801 gaagtcagaa ctgataatgt tgcagctctc cgtcagggaa cctccggtgc aaaagtgcag
     1861 accagcacat caaccggaaa tcaacataaa taccagaccc gtgtggtatc taatgccaac
     1921 aaggttaacc tgaaatttga agaagcgaag cctgtactgg aagaccagct ggctaaatcc
     1981 atcgcgaaca tcctgtaaat tacccatccg gcccggacag attgtaccgg gccgatattc
     2041 ctgccccgga tgaatacatg aaccgtgcag aatctcgtct ctcgcaggta ctctttctgc
     2101 tgggctatga cagtgaaaag tatcctgagc atgtatgtcg ggagttatta aaagaacttt
     2161 accggtttgc tgatgcaacc ggagattctg aactggccat agcctgccag agatacctga
     2221 cagcactgga accgttctct gcatatgaga tcggcgtaag cgcaacagaa tatcttcctg
     2281 tgagtgaata tccgaccgga cgtcgcagaa gaaaatagct gacgcctcgc gttgctcgtt
     2341 gtccaacccc ggaaagtaac aaaataaatt ttgtcacttc ccggcgtttc cgaaataaat
     2401 atgagaacac tgaatggttg tgcaaattct ttgtgcgacc gtgaagtgtt gccggcgaag
     2461 ccggaagctt ccggagaaag tcattatgac atcagacaat atcgctgaat atgttggtga
     2521 gaataatcaa tttactgaga agcctgtaac tgaatacccc ccggtgatac aggataatac
     2581 agtgaccact aaagactatc tgtacagcaa taatattgac actgaccttt tagtggttat
     2641 cggagggctt attgttatat tgtttctcat gatgcttatc atgtacttta ttaattctct
     2701 caggcataag gaattagctg aaatactgac aaaaatatct tttcctgaga aatccgggag
     2761 caatccgctt aagccggaag agggccgctg taatttgttg tccctgtgtg tgctggcccc
     2821 ggtattctgt acccccaaaa agctggcaga agtctccaga cagatcatcg tgaccagtgc
     2881 gcaggaaatt gcctggacgc ccgggagtag ccgtattgat ggtgagcagg aagaaaatat
     2941 tacctggtct ctgtcgtcac agactcatgc cgataaatac gatctcatca ataaaacgtc
     3001 ccgtgtttct cctgacagtg gccactttta tgaaccgggc acggctctgg attatcgtga
     3061 acgggtacgc ctttttattc agatccagtt ttcacacgag agtgactggt ctgaaatatt
     3121 gcgcctgact gatatcgtgc tggaaaaagt ttcttccggc aggtcagcag gcggctatca
     3181 ccaccggtat acagggtata catggtttgt tctgtgaatg tattccacac cgctcttttc
     3241 tgctctgtac ctgactttac cggagtttca tcatgagttt taacgcaaag gatatgaccc
     3301 agggcggtca gattgcgtcc atgcgtatcc gcatgttcag ccagatcgcc aatatcatgc
     3361 tttactgcct gtttattttt ttctggatac tcgttggtct ggttttatgg gtaaaaataa
     3421 gctggcagac gtttgtgaac ggctgtattt actggtggtg taccacgctg gaaggcatgc
     3481 gggatttaat caggtcccag ccggtctatg agatccagta ttacggcaaa accttccgga
     3541 tgaacgcggc tcaggtactg catgataaat acatgatctg gtgcggtgag cagctatggt
     3601 ctgcattcgt tctggcgtct gtcgtggccc tggttatctg cctgatcacc ttctttgttg
     3661 tctcctggat tctggggcgt cagggtaaac agcagagcga aaatgaagtc acaggtggtc
     3721 gtcagctgac tgacaatccg aaagacgttg cccggatgct gaaaaaagac ggcaaggact
     3781 ccgatatccg gattggcgac ctgccgatta tccgggattc tgaaatccag aacttttgtc
     3841 tgcacggaac agtatcgacg ggtaagtcag aggttatccg acgtcttgcc aactatgccc
     3901 gtaaacgtgg tgatatggtg gtgatttatg accgttcgtg tgagtttgtc aaaagttact
     3961 atgatccctc cattgataag atcctgaatc cgctggatgc acgctgtgcc gcctgggatt
     4021 tatggaagga gtgtctgaca cagccggatt ttgataatgt cgcgaacacg ctgatcccga
     4081 tgggcacaaa agaggacccg ttctggcagg gctccgggcg caccatcttt gcggaagcgg
     4141 cgtacctgat gcgtaatgac cccaaccgca gctacagcaa actggtggat acactgcttt
     4201 ccatcaaaat tgaaaaactg cgtacctatc tgcgtaactc accggcggct aacctagtgg
     4261 aagagaaaat cgagaaaacg gcgatttcca tccgtgctgt gctgacaaac tacgtgaaag
     4321 ccatccgtta cctgcagggg attgagcaca gcggtgagtc cttcactatt cgtgactgga
     4381 tgcgtggtgt ccgggaagat aagcaaaacg gctggttgtt tatctcgtcg aatgccgaca
     4441 cccatgcctc cctgaagccg gtggtttcca tgtggctgtc cattgccatt cgtggtctgc
     4501 tggcaatggg agaaaaccgt aaccgtcgtg tgtggttttt ctgtgatgag ttacccacgc
     4561 tgcataaact gccggatctg gtggagatcc tgccggaagc ccgtaagttc ggtggctgtt
     4621 atgtgtttgg tattcagtcc tatgcccagc tggaagatat ctacggtgag aaagcggcgg
     4681 ccacgctgtt tgatgtgctg aatacccgag cctttttccg ctctccgagt cataagattg
     4741 cagagttcgc tgccggtgaa attggtgaaa aagagcacct gaaagccagc gagcagtatt
     4801 cctacggtgc agacccggta cgtgacgggg tatcaaccgg taaggagatg gagcgccaga
     4861 cgctggtcag ttattccgac attcagtctc tgccggatct gacctgttat gtcaccctgc
     4921 ccggaccgta tccggcagta aaactctccc tgaaatatca ggcacgagcg aaggttgctc
     4981 cggagtttat tccgcgtgac atcaatccgg aaatggagaa ccgtctgagt gccgtacttg
     5041 ctgcacggga agcagaaggg cgccagatgg ccagcctctt cgaaccggag gttgcctccg
     5101 gagaaga
//
LOCUS       DAZGXZ010000242         5022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000242 DAZGXZ010000000
VERSION     DAZGXZ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.243"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..344)
                     /locus_tag="ACHKJF_005186"
                     /pseudo
     CDS             complement(1..344)
                     /locus_tag="ACHKJF_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403787.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(322..783)
                     /locus_tag="ACHKJF_005187"
     CDS             complement(322..783)
                     /locus_tag="ACHKJF_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0952382.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQAIPDSPNAERDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERQVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            complement(780..1076)
                     /locus_tag="ACHKJF_005188"
     CDS             complement(780..1076)
                     /locus_tag="ACHKJF_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0952383.1"
                     /translation="MRVRIYIAGPMTGYENFNREAFHRAEEALKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            complement(1073..1354)
                     /locus_tag="ACHKJF_005189"
     CDS             complement(1073..1354)
                     /locus_tag="ACHKJF_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0952384.1"
                     /translation="MILRQCAGTMKVKSIGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLHIVNNYVYFDRRVQA"
     gene            complement(1387..2103)
                     /locus_tag="ACHKJF_005190"
                     /pseudo
     CDS             complement(1387..2103)
                     /locus_tag="ACHKJF_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(2137..2598)
                     /locus_tag="ACHKJF_005191"
     CDS             complement(2137..2598)
                     /locus_tag="ACHKJF_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0952385.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMTQGELKRLAE
                     RQLTKWAKHVGNGMSVPPVRRQLEGAKHPQGPTPIERLKQEYERRKAAGFI"
     gene            complement(2591..3628)
                     /locus_tag="ACHKJF_005192"
     CDS             complement(2591..3628)
                     /locus_tag="ACHKJF_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP0952386.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSISALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGTVNKNSTENGYVSAQNRPKNGTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLDGLSEPIGKFSMTTVWQPSPDFRQRAV
                     VWGMALPEPEFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQVKPVSKGVS
                     HAASGGTASRAVQEIRAAREQWERDNGFISNGNGLEAVGAYGGGVFEPLDSEERGRTF
                     EALDCPDWCDD"
     gene            complement(3600..4151)
                     /locus_tag="ACHKJF_005193"
     CDS             complement(3600..4151)
                     /locus_tag="ACHKJF_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0952387.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRVSDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKRAYRQQY
                     QHYFADKQISVWGSYEYRCTTMG"
     gene            complement(4135..4401)
                     /locus_tag="ACHKJF_005194"
     CDS             complement(4135..4401)
                     /locus_tag="ACHKJF_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0952388.1"
                     /translation="MSHTTLADVIKAVRVSVVADVCGVSQRAIYKWMNNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAVLTIGRIKPTANESLAVNNENHP"
     gene            4481..4900
                     /locus_tag="ACHKJF_005195"
     CDS             4481..4900
                     /locus_tag="ACHKJF_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952389.1"
                     /translation="MVHEDEARKEFASRLALACENAGFEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLKELAALIGTTPTYLLGEDTEESGSLRFYQELSPKQKIIIELLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
BASE COUNT         1151 a         1422 c         1065 g         1384 t
ORIGIN      
        1 ggattttgtc gccctgcgta tccgcgcttt cgcgttacgc tcaatctgaa ttaacttttc
       61 tatatttttc cgcctttcct gttcctcctg gcgcaatagc cttacatcat ctgccagtct
      121 ggtttctctt ttcgccacag agagcatcca gtcaaacggc tccacaactg caccgcagat
      181 ttacagcgga cctgacgctc tttttcgtca acccgaacag aggcgtgatg acaatatggt
      241 ctttccgatg gctcataaag aaaattaacc tgattacgag ggtcatcctc ttttaccgga
      301 aataaaacga tattgcttaa ctcatcctct ggttttattt ccatgctcct ctcctttgat
      361 gcgaatgcca gcgacgcgta atgcgtgttc taggtcaatc aggtaaagcc aactgccatt
      421 ttctttaggt atcatgactt gtcgctcatc tgcatttatc gggtgtccat atcgaaggtc
      481 gtagcgagtc ggtaattgaa cttcccgcgc ttccagttca gcaatgcgct tactcccatc
      541 cgagataaca ccttcgtaat actcacgttg ctcgttgagt tgtgattttg cttcttccag
      601 tccatccagc aaatcagcga taatatccgc ttcccgatga cggatgtgac gcttaaacgc
      661 agcaagagcc gcatcacaat cccgttcagc atttgggctg tccgggatag cctgatacca
      721 cgccagcgtc gactgatagt tttgtgctgc ctcacgaagc gcctcatagt taacctctct
      781 cattgagcca cctcctgata aatcaccgca tgccccagtt tctccgccag tgccagctct
      841 gccttagcgc ccgctgaccg ctgccagcca ttcagcatgt aaatcgcatc cacacaacga
      901 atcattgcca tgcaaatatc catgtagtgc ggctgtgtca gcccgtccgg aagtactgcc
      961 gggtttaaaa cggtatgccc ttcccgtttc agtgcttctt ccgccctgtg aaatgcctcg
     1021 cggttgaaat tttcatatcc cgtcattgga ccggcgatat aaattctcac cctcacgcct
     1081 gaaccctcct gtcgaaataa acgtagttat tcactatgtg cagcggcatg cctaattttc
     1141 tggcaatttc ccttctttgc atgcctctct gatgcagttg ccgcgccagc tcaatatcac
     1201 gctgagaata ttttgccgac gggtgaaaat caccacgtaa catcatactg atgcccagct
     1261 cccgtgcctt cgttctcact gccgcttcag ttcgtccgat aagcgcgcca atgcttttta
     1321 ccttcattgt tcccgcgcac tgccggagta tcagaatttc agcccggcac cacgtcttcc
     1381 acccactcac cgctgctgtt ctctggtggc ggtaatatcc cggagaatat cccggcactt
     1441 gttcagctcc cgcagcgcgg cgcagactcg ctcccacttc tgaacctgac cttttgcccg
     1501 gcgcagctcg cggttagcca catgcagcga tggtaaaatc aggtcatcta ctttcgtttc
     1561 ggtgaccgat ggctgtaact tcacaatgtc ttccacgatt tctgttttca tttcttcctg
     1621 tgtcgtcgtt tcctagactg gtaacgcaac acctgctggc tgaggaaagg ctttaccatc
     1681 ggtgtccgct acggatgcag cttccggctc tgccggtaaa tcagcgcccg gtatgcagta
     1741 acgaaattta ccgccctgat ttacgcgaat cagacgccct ttgctgattg ccatggccag
     1801 cgatgaattc gcccggcggg aggtaatccc gaacataagt gccagctcat ccgccgtttg
     1861 tgggccatgt tgttcaatcg cctcagtcag catttgcgct gtcactttcg gtaccggtga
     1921 cactggttca ctttcaccag cctgaatcag ccaccacatc gcccccttgt tatccgcttc
     1981 accgcggcgc ttcagtttcc acagttcgtt gaccgcatct tcacggctga ttccaaggcg
     2041 ggccgccact acctgtgaag aggctctttt cagtgctttc agtgcgtcaa atacggtttc
     2101 cattaaaatt tcctccgaca aaatcgtttc tcagattcaa ataaaaccag ctgccttccg
     2161 gcgttcgtat tcctgtttca gccgttcaat tggcgttggc ccttgcgggt gtttcgcccc
     2221 ttccagttgt cgtcgcactg gcggaacact catcccgtta ccaacatgct ttgcccattt
     2281 cgtcagttgc cgttccgcaa gtcgttttaa ctcaccctgc gtcatctggc gctcaatccc
     2341 tctggtacgc atttcgaggc agatgtggta cagcacaggc tgtggccacg ggtatttatc
     2401 actcccgtcg tatcgccagg attcattgcg ccagcgccgg tactcttcca tcactgcatc
     2461 caccgtaaga ccaaatggat ttgccccact ctccgaaatc agcgcaacaa actcagccag
     2521 gtccgggggc cacgtttcac ccgcccggca gcggtccatg cactgacggc agaccagacg
     2581 gatttgctgt tcagtcatcg caccaatctg ggcaatccag agcttcgaag gtgcggcccc
     2641 gttcttctga gtccagcggt tcgaatacac ctcccccata agctcccaca gcttccaggc
     2701 cgtttccgtt gctgataaat ccgttgtcac gttcccactg ttcgcgtgct gcccggattt
     2761 cctgaactgc ccgtgatgcc gtgccacctg atgctgcatg gcttaccccc ttgctgactg
     2821 gttttacctg tgccctgacg tgctgcacgt gacgggcaaa tttctgctcc cactgaacct
     2881 gcgtgaaaac cttcccctcc gccatccagt aatcccggaa tgcggcaagc tcagcaggtg
     2941 taaattccgg ctcaggcaga gccatacccc acactactgc ccgttgtcga aaatccggcg
     3001 acggctgcca gacagtagtc atcgaaaatt tcccgatggg ttcgctcagg ccatccaaga
     3061 atacaggggg cgctgcctgt aaagacaaaa cttcctgctc actggtcgga gcactctcgc
     3121 gtgcgttatg tgtggggttt agatctttgg gttcctttgg gttccgtgat ccgtttttgg
     3181 gtgtctttga tggaaaattt gggtgtcttt ggttattttc catgcagcta agagttccgt
     3241 ttttgggtct gttttgtgct gaaacataac cattttcggt actgttttta ttaacagtac
     3301 caattttacc cacctttaaa gactcccgtt tttgggtgta ttcaggctcg gcaacacttt
     3361 cttctacacc gataagtcgg tacaccacaa tttgctttgt tctgcctttt ctctcaccag
     3421 tatcaacaat taacccaatc tccatcaggt gtcgtaaact gtcctgcaca gtctttttgt
     3481 tcagttccgt tacttctgcc agtgcagata tagacgggta tgcgcacaaa tcggcaccgc
     3541 acatatcagc aagccaggtc aatactgact tactggatga actgccggtt ttcacctttt
     3601 tagcccatcg tagtgcatcg atactcatac gaaccccaga cagatatttg tttatctgca
     3661 aagtaatgtt ggtattgctg acgatacgca cgcttgaaag caatggcttt ttctataagc
     3721 tcgtcagtct cacgttccac aacaactgga tccgcaaaaa gcagcccgga ctccaccaca
     3781 tcgccatact ctttgtttaa cccggcgatc atgtacgtaa tgctttttcc gtcagtaatt
     3841 tcacaataca acctgaaatc acttacccgg atagcctcca taattgccgg aatcagcgcc
     3901 gtgaattttt cacgcttatc cctggtgtcg atagccttcc agcgttcgaa tatcttcact
     3961 cgattaacgc ccagcgcccg ttgatcaacc tcgccatcat taaacgtgac gcgttgaaca
     4021 tcgatgttcg ggcgttcttt cagagcccag aatgcttccg tgattaatat cgtcgcctgc
     4081 tcctgtgtca ttcctggtcg gcatacccag gcatccagag cctcacaaac ctgttcaggg
     4141 gtgattttca ttgttcaccg ccagtgattc attcgctgta ggcttaatcc tgccaatagt
     4201 caaaaccgca tcagcagaaa ataatccgtt tgatgccaga gcaatttttt cagcgtaatt
     4261 tgtttcgcct gtatattctg tgcgcggtaa ttttccatta ttcatccatt tgtagattgc
     4321 tctttggctg acaccgcaaa catcggctac aacagaaacg cgaactgctt tgattacatc
     4381 tgcaagtgtt gtatggctca tatcaccctc acgatgtgaa ctttgagtac aaaatataac
     4441 agaactgaca gtacattcaa tagcaaatat cattgaactt atggttcatg aagatgaagc
     4501 gcgtaaagag ttcgccagca ggcttgcgct agcctgtgaa aatgctggct ttgaacaaca
     4561 cggaaggcag gcagaaattg cccgtcgaat gaagctaaca cccaaagcgg ttagtaaatg
     4621 gtttaatgga gagacaattc cccgtcgaga gaaactgaaa gaattggcag cacttatcgg
     4681 aacaacacca acttaccttc tgggggagga cactgaagaa agcggaagcc tgcgtttcta
     4741 tcaggagtta agtccgaagc aaaaaatcat cattgaactt cttgatgaac tccctgacag
     4801 tgaaactgat gaacttttaa aaactcttga ggagaaaaaa cagaagtaca acgcaattta
     4861 tgaagaatta gcgcgaaaga aaaaacaaaa agcctcctga atctgcataa atccggtagc
     4921 ttccctccgg gtttatgctc ctctcatccc ctcctgatat tttatccctc acaaaaatgt
     4981 actaaaagta ctttacatta atgaacaaca agtacattat gt
//
LOCUS       DAZGXZ010000243         4899 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000243 DAZGXZ010000000
VERSION     DAZGXZ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.244"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(306..1337)
                     /gene="rsmC"
                     /locus_tag="ACHKJF_005196"
     CDS             complement(306..1337)
                     /gene="rsmC"
                     /locus_tag="ACHKJF_005196"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP0952390.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFIVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1440..1853
                     /gene="holD"
                     /locus_tag="ACHKJF_005197"
     CDS             1440..1853
                     /gene="holD"
                     /locus_tag="ACHKJF_005197"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP0952391.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1822..2268
                     /gene="rimI"
                     /locus_tag="ACHKJF_005198"
     CDS             1822..2268
                     /gene="rimI"
                     /locus_tag="ACHKJF_005198"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0952392.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2283..2960
                     /gene="yjjG"
                     /locus_tag="ACHKJF_005199"
     CDS             2283..2960
                     /gene="yjjG"
                     /locus_tag="ACHKJF_005199"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP0952393.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3346..4581
                     /locus_tag="ACHKJF_005200"
     CDS             3346..4581
                     /locus_tag="ACHKJF_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0952394.1"
                     /translation="MALSDAWLRSVVGKKRDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELELNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IVDRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEINILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQFAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYWAWW
                     ERVESIVTCTDLISNGQFA"
BASE COUNT         1242 a         1174 c         1253 g         1230 t
ORIGIN      
        1 cctgaagcta gcgcgtctac caattccgcc accttcgcat accatcaatt cttaaaaaga
       61 attgctacca cggaggcgca ttctagtggt tttcagcttt tcgtcaatag ttaattatcg
      121 ccggaggtgt aattgctgga aaaatgtcca tcaggaaact agcgtgcagg tttggtaggc
      181 atgcgggggc agatgccaga tgcgacgctg gcgcgtctta tctggcctac gaagggctaa
      241 cgtgcaggtt ttgtaggtcg gataaggcgt tcacgccgca tccgacacgg tattcggcga
      301 gataattaac ctttcttcgc ctggcgggtc ataatggcgc gatacacctt gaaacgcccg
      361 gtttgcgcaa tcacttcatg gaagccaaat gtctcatcca gcacgtccgg gtaaggcagg
      421 aaggcgttcg ctacaattcg cagctcgccg ccgctattaa gatgacgcac cgcgccgcga
      481 atcagcgttt gcgccgcatc caggctggtt tgcatcccat cgtggaacgg cgggttggag
      541 atgatcatat caaaacgacc tttcacctcg gaaaagacgt tgctggcaaa gacttcacct
      601 tcaacacagt tggccgcaag tgttgcgcgg ctggcttcta ccgctggcgc agagacatcg
      661 cacaaggtga gacgaatttt cggcgaatgg cgcgcaaagg caactgaaag cacccccgcg
      721 ccacagccga catccagcac tttacctttc gtgtgcggag ttaacgtcga gagcagcaac
      781 tggctaccga catccagacc gtcgcggcta aacacgccag gcagcgtttt gaccgtcagg
      841 ccatcgacgc tgtattcgcc ccagaattta tcggcatcaa ataccggctg tttttccaga
      901 cgaccaaaat agaggccaca gcgacgggcg ctgtcgactt tattcaacgg cgcataatca
      961 gccagcatct gctcggcgct gcgcacgccg ctgcgattct cgccaacgat aaaaatatct
     1021 gtccccactg gcagcagaga aagtaaattc atcaactgga actgggcttc cggtttgttc
     1081 ttcggccagt agtaaatcag cgtatcgcaa tctgcgacgt catccgccgt ggcgaccaga
     1141 ctgaaacggg cgttatcccc catctggcgg cttaatacct gccagtggtg gaattgctgg
     1201 gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca ggtcatcctg taagtctccg
     1261 gcaaacagaa tacggctttg ttcgaaatca tcactgtgac gcagcaagac ttcacttgcc
     1321 ggggtaaatg cagacatgga atgctcctca attgatactg gcggcgatta tagccatatg
     1381 ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg acaacaggag cgattcgcta
     1441 tgacatcccg acgagactgg cagttacagc aactgggcat tacccagtgg tcgctgcgtc
     1501 gccctggcgc gttgcagggg gagattgcca ttgcgatccc ggcacacgtc cgtctggtga
     1561 tggtggcaaa cgatcttccc gccctgaccg atcctttagt gagcgatgtt ctgcgcgcat
     1621 taaccgtcag ccccgatcaa gtgctgcaac tgacgccaga aaaaatcgcg atgctgccgc
     1681 aaggcagtcg ctgcaacagc tggcggttgg gtactgacga accgctatca ctggaaggcg
     1741 ctcaggtggc atcaccggcg ctcaccgaat tacgggcaaa cccaacggca cgcgccgcgt
     1801 tatggcaaca gatttgcaca tatgaacacg atttcttccc tcgaaacgac tgatttacca
     1861 gcggcttacc acattgaaca acgcgcccac gcctttccgt ggagtgaaaa aacctttgcc
     1921 agcaaccagg gcgagcgtta tctcaacttt cagttaacgc aaaacggcaa aatggcggcg
     1981 tttgcgatta cgcaagtggt gctggatgaa gctacattgt tcaatattgc ggtcgatcct
     2041 gactatcagc gtcagggatt gggaagggtg ctgctggaac atctgatcga cgaactggaa
     2101 aaacgcggcg tggcgacact atggctggaa gtccgtgcct caaatgctgc cgccattgcc
     2161 ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc gcaattacta ccccaccacg
     2221 gacggtcgcg aagacgccat catcatggcg ttgccaatca gtatgtaaga caaggtggaa
     2281 taatgaagtg ggactggatt ttctttgatg ccgatgaaac gctgtttacc tttgactcat
     2341 tcaccggcct gcagcggatg tttcttgatt acagcgtcac ctttaccgct gaagattttc
     2401 aggactatca ggccgttaac aagccactgt gggtggatta tcaaaacggc gcgatcactt
     2461 cattacagct tcagcacggg cgttttgaga gctgggccga acggctgaaa gttgaagcag
     2521 gcttgcttaa cgatgccttt attaatgcga tggcggaaat ctgtacgccg ttgccgggcg
     2581 cggtttctct gcttaacgcc attcgtggca acgctaaaat cggcatcatc accaacggtt
     2641 ttagcgcctt gcagcaagtg cgtctggaac gcacgggcct gcgtgattac ttcgatttgc
     2701 tggtgatttc cgaagaagtt ggcgttgcca aaccgaataa gaaaattttc gattatgcgc
     2761 tggaacaggc gggcaatcct gaccgttcac gcgtgctgat ggttggcgac actgccgagt
     2821 ccgatattct cggtggcatc aacgccgggc tggcgacctg ctggctgaat gcgcaccatc
     2881 gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc atcgttgcac gaactggagc
     2941 agctcctgtg taaacactga ttgcctcccc cccgttgatg ggtaaaatag ccgcaatttt
     3001 tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga agaaagaatt atgacgttgt
     3061 ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc cattatttct caccccaata
     3121 attaagccta aattaaagct cttttacttt ttcaaagtcc tttcagttaa ttgatgtttt
     3181 gttttataag tcatttaaat acagttagtt atgtggcttc tttaattgaa aattctttca
     3241 tgtaacttta aatcgatcag gttgctttca tcaaaaatct gtacatatgc ttgtacataa
     3301 tgtacaaagc aacagaggtg ttttgcgatt tgtacaaggt gagtaatggc gctgtctgat
     3361 gcgtggttgc gttcagtcgt tggaaagaaa cgtgataagg ttttggttaa atccgatcgt
     3421 gatggtctgt ctgtcagagt atcaccgaaa ggtcgcgtag tgttccaata tcgttatcaa
     3481 tgggcaggga aaggtgagcg ccttgatatc ggaacttacc cggcaactgg attaaaagag
     3541 gccagagaag aagttatccg tctccgtggt gaactcgagt taaaccgcaa tccacgatta
     3601 gtcaagcagg ctgaaaaacg aaaagctact gaagccatga cggtagagtc tgtgatccgt
     3661 gcctggtatg aagcatattg tgtaaaaaat aaaaaaggtt ctgaacagat actccgctcg
     3721 tttgagctgc acctgttctc taaaatcggg aatatccctc acgatgcagc tacattgcat
     3781 gattggttag aagtcctgga gcctcttagc accaagactc cagcaatagt agaccgattg
     3841 ctaattaacg caaagcaggc tcatgtctgg gcgtataaga gaaagctcat tgaaactcgc
     3901 ccactgtcgg atatcacggg taaagatatg gatatccgta aaggtcagaa aaaacggttt
     3961 ctgacacatg atgaaattaa catcctttat gctgcgatcg atggttctcg aatggttcct
     4021 aaataccgag ccttcattaa actattgctg cattttggtt gccgtagttc agagctaatt
     4081 actgctaggg tggatgattt tgatttcata aataaggtat ggactgtacc accagaacga
     4141 cataagactg gggacataac aggcgaaccg ctaaagcggc ccattattga accggttgaa
     4201 gagcttataa aatacgttat ctctatgaac aacggttccg atatgctttt taccaaggaa
     4261 ggaagcaggg agccagttgg tcggacatca ttgcagtcgc tgccttacaa tttaatgcag
     4321 ttcgcatggc gacgtttggg gtatcaattt cctcattggt ctcttcatga tttgagacga
     4381 acagcacgaa caaacttttc tgatcttact gctcctcata ttgctgaaat aatgctcggt
     4441 cataaactgc caggtgtatg gcaagtttat gataagagcg attatctaga agaacagcgt
     4501 aaagcttact gggcatggtg ggagagggtt gaatcgatcg ttacttgtac tgacttaatc
     4561 tccaacggac agtttgcgta gccagagcgt aatcatatct gccagtccgc tgcggatacc
     4621 ttgtgtactg ggtttttagc cagactacgt taaccggtag cttcagtatt tgggggggcg
     4681 gcaggagttt gatggtttat tgggttactg tgggactaag gatgtaggga tgccaatcaa
     4741 tgaattatga aaaatatcat agcacaaaca gaagcaaggg tttgcgaacc ttctgctact
     4801 taatttatta tgtgcaggtg atttttaatg tgatgaagtg ttttatgact cgcggaaact
     4861 ttgattttat aaactctaac gtggttttct cagaagccg
//
LOCUS       DAZGXZ010000244         4862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000244 DAZGXZ010000000
VERSION     DAZGXZ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.245"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..585
                     /locus_tag="ACHKJF_005201"
     CDS             <1..585
                     /locus_tag="ACHKJF_005201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0952395.1"
                     /translation="ISYELTPQEYNAIELLNSNNLMWGTYNLKKLFGPAIRGKRLKNA
                     DRIPGSLPFVTAGEGNEGISAFISNDVVVFPENTITIDMFGSAKYRNYEYGADDHVAV
                     VHTEFFDRNAVIFITASIHKSSCTGKFNYGRNFYAKDADELMVSLPSKDNNPDYSFME
                     TLILAVHKLVIKDVVQYTDKKLVAYRQITSEKVV"
     gene            complement(941..1159)
                     /locus_tag="ACHKJF_005202"
     CDS             complement(941..1159)
                     /locus_tag="ACHKJF_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013575645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952396.1"
                     /translation="MLIYNVFGRHIGVKREGDHWLVFRADLTERKFSRLYDIVIPDDM
                     TEDEIPGWLSDIFHEAATERHPDVKRIR"
     gene            2032..3945
                     /locus_tag="ACHKJF_005203"
     CDS             2032..3945
                     /locus_tag="ACHKJF_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0952397.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSDRDISSPAEPGSHPTVSTKPAAEPEYYYVIALAGQSNGMSYGEGLPLPDT
                     FDSPDPRIKQLARRSTVTPGGAACIYNDIIPADHCLHDVEDMTGLNHPKADLSKGQYG
                     CVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGTDGTYSDTTGASESSTRWGVD
                     KPLYKDLIGRTKAALAKNPKNVLLAVVWMQGEFDFSGTPANHTAQFGALVDKYRADLA
                     DMVGQCVGGSADGVPWICGDTTYFWKQKNETAYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GANVPTNKPEEDPDIPGIGYYGSKWRTDRSTWTSQDRASHFSSWARRGIISDRLATAI
                     LSCAGKSSAFVNGTAGVVVPDRPVTTSESVIFYDAKKASDNQLKPYGWDGMDGRRTLV
                     DDSGNKALRIEKNNSAKSWSMYCDIAADKAKLLLEKGGEIAVRFKIPENVNLETTRNK
                     YAFGLYWRIAEWPGEGGEGYLSSFFVQTDKASIDVAYHHTVNQQKELGTFGAFDHDWH
                     TLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANELNRFVITDITGSAETYPVLID
                     MVEVKANKAGAAA"
     gene            4088..4270
                     /locus_tag="ACHKJF_005204"
     CDS             4088..4270
                     /locus_tag="ACHKJF_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0952398.1"
                     /translation="MIFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLIP"
     gene            4296..4568
                     /locus_tag="ACHKJF_005205"
     CDS             4296..4568
                     /locus_tag="ACHKJF_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0952399.1"
                     /translation="MSEIKPLVTAEAVKDVIRSEEVRSALKQKLRQNLEERLDAEVDA
                     ILDELLGVPAEPPTEAGDTTAENGKPQPELPVADATEPQPETVMML"
     gene            4645..4860
                     /locus_tag="ACHKJF_005206"
     CDS             4645..4860
                     /locus_tag="ACHKJF_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0952400.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
BASE COUNT         1303 a         1061 c         1269 g         1229 t
ORIGIN      
        1 ataagctatg agcttacacc gcaggaatat aacgcgattg agctattaaa tagcaataat
       61 cttatgtggg gcacttataa cctcaaaaag ttatttggcc ccgcaataag aggtaagcgt
      121 ctgaaaaacg cagatcgtat tcccggatcc ttgccttttg ttactgcggg tgaaggcaat
      181 gaaggtattt ctgcatttat cagtaatgat gttgttgtat tccctgaaaa taccattacg
      241 atagatatgt ttggctccgc aaaatacaga aattatgagt atggtgcaga tgaccatgtg
      301 gcagttgttc atacagaatt ctttgacaga aatgccgtta ttttcataac agcatcaatt
      361 cataagtctt cttgtaccgg aaagttcaac tacgggagaa atttctatgc aaaagatgcc
      421 gacgagttaa tggtatcttt gccgtcaaaa gataataacc ctgattattc tttcatggag
      481 acgctaattc tagcagtaca taaactggtc atcaaagatg ttgtccagta cactgataaa
      541 aagctggtag cgtacaggca gattacatct gagaaggtcg tttagtgcaa atccccacct
      601 gacagggtgg ggaatggtca ggttatcgtg ccctgatgca gaaaaacagt ttacaatcgt
      661 aaaaatcatc atatcatgac aaaagtggtt acattgccac gcagccgaac ccgccgatgc
      721 gcgggttttt tgtatccgga atcctgtgag ttatacggaa agtacacaga aaggaaggtg
      781 cgaccgcaat taataacaaa atcttaaaaa tcgcatatgg cactattagt tttctaaata
      841 ttgtatattt tgagtattgc aggatatccc ctgtaacgaa gtttgcgtaa cagcatttta
      901 ctctacgagt ttgccagcct cccccagtgg gctggctttt ttatctgatg cgcttcacat
      961 cagggtgacg ttctgttgcc gcttcatgga aaatgtcgct gagccagccc ggtatctcgt
     1021 cttctgtcat atcgtctgga atgacaatat cgtataaccg ggagaatttt cgttctgtca
     1081 ggtctgcccg gaagaccagc cagtgatcac cttcacgttt gacaccaata tgtcgaccaa
     1141 agacgttgta gattagcact atcgacctct ttcaggaatt gtgtaactga gatgacagat
     1201 tcagatttcg tcaataaatg caaaatgtca atcctgagat catggtgagc tatctggcgg
     1261 ttttttaata tctgtttaac gggggatgtt acatacctca caattcagtc agttgaaggt
     1321 tgtctgcccg gatgagaatt tgttagaaaa tttctgcatg gtgaatcccc ctgtgcggag
     1381 gggcatatca gcgacaggtg gttggttata tcccttatcc tttctgtgcg ggttcaggtg
     1441 ctgatactga actcaccggg aggcacccgg caccatgcgc atgatgatac agatacgcgg
     1501 ctttagcccc tctccggagg ggctttttaa tgaggggaag gcaccgggca gtgaaatgtt
     1561 aaatcactca caattcagtc agttgacggt tgcctgtctg gctgagcatt tgttaaaaaa
     1621 atcctgcatg atgaatcccc ctgagcggag gggcataatg acagatgttt ggttgcgtat
     1681 tgtataggca agttgcggat tctgtctggt cattgcagaa ttcaccggga ggcacccggc
     1741 atcatgctgt atacagagat taggcatata tccaggctcc tcatcgcagg agccttttta
     1801 catgcaaaaa aagcccgcgc cgggagacgc gggcagcaag gaataaacaa caaaacgtga
     1861 agtaatcaat ttttcagctg gcgaataata cccgacagta atcactctgc gcaactgcgc
     1921 ggcctttttc gtattgcggg ctgtagtctt ccttctgtca ttgtcctgta acttccggac
     1981 ttcagcccgc cccttatctg actcacaaca ttatcccggc cgggaggatt catggcattt
     2041 aaacactatg acgtggtcag ggcggcgtcg ccgtcagacc ttgcggaaaa actgactcaa
     2101 aaactgaagg aagggtggca gccttatggt agtgccctga tttcgacagc tggttatggt
     2161 gcggagttta tccagcctgt tgtgagtgac agggatatct catcacctgc ggagccaggc
     2221 agtcacccga ccgtttcaac gaagcctgca gccgaaccag aatattacta tgtgatcgcg
     2281 cttgctggtc agtccaatgg tatgtcatac ggtgaggggc tgccattacc ggatacattc
     2341 gacagccctg atccacgtat taaacagtta gcgcgtcgca gtacggtgac accgggaggt
     2401 gctgcatgta tctataacga tattatcccg gcagatcact gtctccatga tgtggaggat
     2461 atgacagggc ttaatcatcc caaagcggat ttatcgaaag gtcagtacgg ctgtgtggga
     2521 caggggctgc atatcgccaa aaagcttctg ccatttatac cggcgaatgc gggcattctt
     2581 cttgttccgt gctgccgtgg tggttcagct ttcaccaccg gaactgatgg cacatacagt
     2641 gacactactg gcgcctcaga gagttccacc cgctgggggg tggacaagcc gctgtataag
     2701 gacctcattg gtcgtacaaa agccgcgctg gcgaagaatc cgaaaaatgt gctgcttgcc
     2761 gttgtgtgga tgcaggggga gtttgatttc agcggcacgc cagcgaatca cacggctcag
     2821 tttggcgcac tggtggataa ataccgtgcg gaccttgcag atatggtggg acagtgtgtt
     2881 ggtggttctg ctgacggtgt tccctggatt tgtggagaca caacatattt ctggaagcag
     2941 aagaacgaaa cggcgtacca gacggtgtac ggtagttaca aaaacaaaac ggaaaagaat
     3001 atccattttg tgccgttcat gaccgatgag aacggggcaa acgtcccgac gaacaaaccg
     3061 gaagaagacc cggatattcc gggtatcggg tattacggtt caaaatggcg aacggacaga
     3121 agcacctgga catctcagga cagggccagt catttcagtt catgggctcg ccgtgggatt
     3181 atttccgacc gtctggcaac ggcgattctg agctgcgcgg gtaagtcttc tgcgtttgtt
     3241 aatggtactg ccggggtggt tgttccagac agaccggtta ccacctcaga gtctgtaatt
     3301 ttttacgatg ccaaaaaagc ttcagacaat cagctgaaac cttatggctg ggacggtatg
     3361 gatggcagac gcacactggt tgatgacagc ggcaataaag ctctgcgaat tgagaaaaat
     3421 aacagcgcga aatcctggtc aatgtactgt gatattgctg cagacaaggc aaaactttta
     3481 ctggaaaaag gcggggaaat tgctgtccgg tttaaaatcc ccgaaaacgt caatcttgag
     3541 acaaccagaa acaagtatgc ctttggtttg tactggcgaa tagcggaatg gccgggtgag
     3601 ggtggtgaag gctatctgag ttctttcttt gtccagacag ataaagccag tattgatgtt
     3661 gcataccatc atacagttaa tcaacaaaaa gaacttggca cgtttggcgc attcgaccat
     3721 gactggcata cgcttgcatt taaatttaag ggcagtaaca gcattaatgt tactccggtg
     3781 cttgatggtg tggatggaca ggcgtttgac ctggtgaaat gggccaatac tgctaatgaa
     3841 ctcaacaggt ttgtcattac ggatattaca ggtagtgcag aaacctaccc tgtacttatt
     3901 gatatggtgg aagttaaagc aaacaaagct ggagcagccg cataattgct aaaaaaagcc
     3961 gccagcggca ggaatggaag ctggcggagg taatcccaat ggagaatgta aagaaaagat
     4021 gctttcgtac atcggttatt ttttaaatga aaacagttct cattgtcaac cataacggta
     4081 agaaattatg atatttattc atcaggtgat gctgtacttc tgtacggcgg tctgtgtgct
     4141 gtatcttctt tcgggtggat acagggctgt gcgcgatttc tggcgcaggc agattgataa
     4201 aagggccgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagagccgct
     4261 cattccttaa caaccccttt cagcgagaaa atcctatgtc agaaattaaa cccctggtca
     4321 ctgcagaggc agtgaaggac gtcattcgct ctgaagaagt cagaagcgca ctgaaacaga
     4381 aacttcgcca gaatctggag gaacgcctcg atgctgaagt ggatgccatt ctggatgagc
     4441 tgctcggcgt accggcagag ccaccgactg aagcgggaga taccaccgca gagaacggta
     4501 aacctcagcc tgaattaccg gtagccgatg caactgaacc gcaacctgaa acggtcatga
     4561 tgctgtaacg ggctgtcagg gccgccagta aaccactggt ggtcttttta ttgttgtgag
     4621 cttccgttgc gcgggagacg gggtatgtac cagatggaaa aaatcacaac aggtgtgtca
     4681 tacaccacgt cagcggtggg gacgggctac tggttcctgc agttgctgga cagggtttcc
     4741 ccgtctcagt gggcggcaat aggcgtgctg gggagtctgc tgtttgggct gctgacatat
     4801 ctgacgaacc tgtatttcaa gattaaagaa gaccggcgta aggcggcgcg gggagagtga
     4861 at
//
LOCUS       DAZGXZ010000245         4698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000245 DAZGXZ010000000
VERSION     DAZGXZ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.246"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..347)
                     /locus_tag="ACHKJF_005207"
     CDS             complement(<1..347)
                     /locus_tag="ACHKJF_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFP0952401.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSP"
     gene            651..1583
                     /gene="rfaD"
                     /locus_tag="ACHKJF_005208"
     CDS             651..1583
                     /gene="rfaD"
                     /locus_tag="ACHKJF_005208"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP0952402.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1593..2639
                     /gene="rfaF"
                     /locus_tag="ACHKJF_005209"
     CDS             1593..2639
                     /gene="rfaF"
                     /locus_tag="ACHKJF_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP0952403.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLYKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2643..3635
                     /gene="rfaC"
                     /locus_tag="ACHKJF_005210"
     CDS             2643..3635
                     /gene="rfaC"
                     /locus_tag="ACHKJF_005210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP0952404.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3632..>4698
                     /gene="rfaL"
                     /locus_tag="ACHKJF_005211"
     CDS             3632..>4698
                     /gene="rfaL"
                     /locus_tag="ACHKJF_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP0952405.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTTKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKN"
BASE COUNT         1261 a         1040 c         1114 g         1283 t
ORIGIN      
        1 tctggtgacc agtactctgg gttttttaat tgtcgaggct caagtctatt tgtaaacgac
       61 tcatcttttt gaattttttc gatacgactt ctgatatatc taaatttctt ttttatatcg
      121 ataacgataa ccgttgttgg ttttccgttg cgaatcgctt caacccttgg tttcaaatcg
      181 ggagaagtga aaataatcat ctcattttca agtgcggcca gacgctcaaa gtaactaaaa
      241 taaacatcaa ctgaacgagc tagtttttca cggaaccctt tattagcagt ccaatcccct
      301 ctaccaatgt caaaatatgc agtgataatt gtggtggatg atttcattac taataagacc
      361 attaatataa aaaaagaatt atatcataaa tattaacaga atgtatactg tatattatcc
      421 attcaaatgc ttccgttatt tatcatacat cggaatattg atactaaagc actattcaca
      481 tgcaaaacca acatccgcca tgaaggacta gctaaaaccc aaactagttt gttgcaatta
      541 gcatccttgc acctctatgt aaagggctga agggattcgg atgtgatggt atgattacag
      601 acattcgtgt ctgagattgt ctctgactcc ataattcgaa ggttacagtt atgatcatcg
      661 ttaccggcgg cgcgggcttt atcggcagca acatcgttaa ggccctgaat gataaaggca
      721 tcaccgatat tctggtggtg gacaacctga aagacggcac caagtttgtg aacctggtgg
      781 atctggatat cgcggactat atggataagg aagacttcct gatccagatt atggctggcg
      841 aagagttcgg cgatgtcgaa gcgattttcc acgaaggcgc atgctcttcc accaccgagt
      901 gggatggcaa gtatatgatg gataacaact atcaatactc caaagagctg ctgcactact
      961 gtctggagcg cgaaatcccg ttcctgtatg catcttccgc ggccacctac ggcggacgca
     1021 cctccgactt tattgaatcc cgcgagtacg aaaaaccgtt gaacgtctac ggttactcaa
     1081 aattcctgtt tgatgaatat gttcgtcaaa tcctgcctga agcgaactcg cagattgttg
     1141 gcttccgtta tttcaacgtt tatggaccgc gtgaaggcca taaaggcagc atggcgagcg
     1201 tcgctttcca tctcaacacc cagcttaaca acggtgaatc accgaagctg tttgaaggta
     1261 gcgagaactt caaacgcgac ttcgtctatg tgggcgacgt ggctgatgtg aatctgtggt
     1321 tcctggaaaa tggcgtttcc ggcatcttca atctcggtac tggtcgtgcg gaatccttcc
     1381 aggctgtagc tgatgctacg ctggcttatc acaagaaagg ccagatcgaa tacattccgt
     1441 tcccggataa actgaaaggc cgctatcagg cgttcactca ggcagatctg acgaatctgc
     1501 gcgcggcggg ttacgacaaa ccgttcaaaa ccgttgctga aggtgtaacg gaatacatgg
     1561 cctggctgaa tcgcgacgca taagagctct gcatgaaaat actggtgatc ggcccgtctt
     1621 gggttggcga catgatgatg tcgcaaagtc tctatcgcac gctccaggcg cgctatcccc
     1681 aggcgataat cgatgtgatg gcaccggcat ggtgccgtcc attattatcg cggatgccgg
     1741 aagttaacga agctattcct atgcctctcg gtcacggagc gctggaaatc ggcgaacgcc
     1801 gcaaactggg tcatagcctg cgtgaaaagc gctacgaccg cgcctacgtc ttacccaact
     1861 ccttcaaatc tgcattagtg cctttcttcg cgggtattcc tcatcgcacc ggctggcgcg
     1921 gcgagatgcg ctacggttta ctcaacgatg tacgcgtgct ctataaagaa gcctggccgc
     1981 taatggtgga acgctatata gcgctggcct atgacaaagg cattatgcgc acagcacaag
     2041 atctgccgca gccattgtta tggccgcagt tgcaggtgag cgaaggtgaa aaatcatata
     2101 cctgtaatca attttcgctt tcatcagaac gtccgatgat tggtttttgc ccgggtgcgg
     2161 agtttggtcc ggcaaaacgc tggccacact accactatgc ggagctggca aagcagctga
     2221 ttgatgaagg ttatcaggtg gttctgtttg gctcggcgaa agatcatgaa gcaggcaatg
     2281 agattcttgc cgctttaaat accgagcagc aggcatggtg tcggaacctg gcgggggaaa
     2341 cacagcttga tcaagcggtt atcctgattg cagcctgtaa agccattgtc actaacgatt
     2401 ctggcctgat gcacgttgcg gcggcgctca atcgtccgct ggttgccctg tatggtccaa
     2461 gtagcccgga cttcacaccg ccgctatccc ataaagcgcg cgtgatccgt ctgattaccg
     2521 gctatcacaa agtgcgtaaa ggtgacgctg cggagggtta tcaccagagc ttgatcgaca
     2581 ttactcccca gcgcgtactg gaagaactca acgcgctatt gttacaagag gaagcctgac
     2641 ggatgcgggt tttgatcgtt aaaacatcgt cgatgggcga tgttctccat acgttgcccg
     2701 cactcactga tgcccagcag gcaatcccag ggattaagtt tgactgggtg gtggaagaag
     2761 ggttcgcaca gattccttcc tggcacgctg ccgttgagcg agttattcct gtggcaatac
     2821 gtcgctggcg taaagcctgg ttctcggccc ccataaaagc tgaacgcaaa gcgtttcgtg
     2881 aagcgctaca agcagagaac tatgacgcag ttatcgacgc tcaggggctg gtaaaaagcg
     2941 cggcactggt gacacgtctg gcgcatggcg taaagcatgg attggactgg caaaccgctc
     3001 gcgaaccttt agccagcctg ttttataatt gtaagcatca tattgcaaaa cagcagcacg
     3061 ccgtagaacg cacccgcgaa ctgtttgcca aaagtttggg ctatagcaaa ccgcaaaccc
     3121 agggcgatta tgctatcgca cagcattttc tgacgaacct gcctacagat gctggcgaat
     3181 atgccgtatt tcttcatgcg acgacccgtg atgataaaca ctggccggaa gaacactggc
     3241 gagaattgat tggtttactg gctgattcag gaatacggat taaacttccg tggggcgcgc
     3301 cgcatgagga agaacgggcg aaacgactgg cggaaggatt tgcttatgtt gaagtattgc
     3361 cgaagatgag tctggaaggc gttgcccgcg tgctggccgg ggctaaattt gtagtgtcgg
     3421 tggatacggg gttaagccat ttaacggcgg cactggatag acccaatatc acggtttatg
     3481 gaccaaccga tccgggatta attggtgggt atgggaagaa tcaagtagag tgtcgttcta
     3541 caagtatgtc tcttgcagat ttgccagctc aaaccgtttt tcaaaattta aatcttgaaa
     3601 taataaccaa taagttgaca tcggagataa gatgacctca acattatttt tctctctcga
     3661 gaaaaaaaac tggatagcgt actggaacag agctctcgta ttcttattca ttaccaccta
     3721 ttttttgggt gggattacaa ggtataaaca tcttattgtt attcttatga caataacgac
     3781 aatcgtctat ctctgcaaac ggccaaaaca ctatctctca ctatttaaaa catttctttt
     3841 tggtagtgtt gccatattaa ctattgctgc attgctgtca cttcttcaat cccctgatgc
     3901 aggtgctagc atgaaggaaa ttttcaaagc tattattgag aatactttac tatgcacaat
     3961 agcaataccg atcatattga gagacgagaa aagagaagat gtcgaaaaaa tcgttttctt
     4021 ctcatttatt agtgcgttgg gcttacgctg tttttctgaa ttgattacct attataagga
     4081 ctatcaacaa gggataatgc cattcgcaga ttatagacac cgtagcattt ctgactcgat
     4141 ggtcttttta ttccctgcat tgttaaatct ctggcttatc aaatcagcaa aataccgcat
     4201 ttcttttgtg gttctaagcg ttatttttat ttttctgata ttaggaactt tatccagagg
     4261 ggcctggctt tccgtgttag tcattggatt aatatggatt ctgatgttta aacaatggaa
     4321 gttactatta gtaggagtaa tggttgccat cattgcattg tcggttattt tcacacataa
     4381 ggagatgacc acaaagctaa cgtataaact tcaacaaact aatagttctt atcgctatgc
     4441 aaatggtact caaggcagcg cactcgatct aatattagaa aatcctgtta ttggttatgg
     4501 ttacggtaac gttgcatata aagatgtcta taataaacgt gtcattgatt atccagaatg
     4561 gacctttaga caatcaatag ggccacataa ttttgcgcta ttcatctggt ttggcactgg
     4621 tttattaggg ctggtaagtc ttatgatgct atactgtgca atattgaaag agtgtataaa
     4681 aaatggcgtc aagaataa
//
LOCUS       DAZGXZ010000246         4515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000246 DAZGXZ010000000
VERSION     DAZGXZ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.247"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(71..268)
                     /locus_tag="ACHKJF_005212"
     CDS             complement(71..268)
                     /locus_tag="ACHKJF_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0952406.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLLHQNR"
     gene            complement(280..768)
                     /locus_tag="ACHKJF_005213"
     CDS             complement(280..768)
                     /locus_tag="ACHKJF_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0952407.1"
                     /translation="MKLTLTPEADSVNVQALNMGRIVVDVDGVNLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(765..1142)
                     /locus_tag="ACHKJF_005214"
     CDS             complement(765..1142)
                     /locus_tag="ACHKJF_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP0952408.1"
                     /translation="MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITQGKHPEAKQ"
     gene            complement(1189..1566)
                     /gene="cbeA"
                     /locus_tag="ACHKJF_005215"
                     /pseudo
     CDS             complement(1189..1566)
                     /gene="cbeA"
                     /locus_tag="ACHKJF_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
     gene            complement(1642..1863)
                     /locus_tag="ACHKJF_005216"
     CDS             complement(1642..1863)
                     /locus_tag="ACHKJF_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0952409.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     NIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(1932..2408)
                     /gene="radC"
                     /locus_tag="ACHKJF_005217"
     CDS             complement(1932..2408)
                     /gene="radC"
                     /locus_tag="ACHKJF_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0952410.1"
                     /translation="MQQLSFLPGAMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(2423..2896)
                     /locus_tag="ACHKJF_005218"
     CDS             complement(2423..2896)
                     /locus_tag="ACHKJF_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0952411.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(3030..3230)
                     /locus_tag="ACHKJF_005219"
     CDS             complement(3030..3230)
                     /locus_tag="ACHKJF_005219"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952412.1"
                     /translation="MKHIPEGVIQAPVSPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNG"
     gene            complement(3230..4051)
                     /locus_tag="ACHKJF_005220"
     CDS             complement(3230..4051)
                     /locus_tag="ACHKJF_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0952413.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            complement(4229..4360)
                     /locus_tag="ACHKJF_005221"
     CDS             complement(4229..4360)
                     /locus_tag="ACHKJF_005221"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0952414.1"
                     /translation="MAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIRNAEGQRR"
BASE COUNT         1005 a         1173 c         1289 g         1048 t
ORIGIN      
        1 tttgcagata aatgaaaacg ccagccctgt tcaggctggc gtcagtggaa tgaagtctgg
       61 tgagtgagag tcaccggttc tggtgcagaa gctgcaactg gcgcagtgta cggatatctt
      121 ccctggcctg ttcaatgcag ggcagcagtg ctgccgaatc cgttttatcc tcatcgttgt
      181 caaagataat tcccgattcg cagtcgatat tgtcctgcag ccacgcaatc agaatatcca
      241 gcgctgtttc cgtggttaat gatttcatgt tgtttctggt taccagtcga aagtgggtaa
      301 acccggcagg cattcagcgc tggcatccag atgaatgaga ctgacgccat aacgtcgggt
      361 aagtgtgaca accagacggc ggaacgtttt tgacagcccc aggcgtttaa gccgcagaac
      421 cgggtatgac cacgcatcgg tacgtaacag atatccgcta cctgtgaaat gaatccattc
      481 tgattcgccg gtgtcattcg tctgatgtga cagcgagtac agccaggcgt tgtcctttgc
      541 agtgatatgc gcggtactgc agcgtatgcc ggtaagggcc gcagatggtg gcgtgcgctc
      601 tgctgatgtc cggtcagatt catcaacaac acgaagggag taaccgttat cgcagaccac
      661 gttaatcagt tcagcgagat taacaccatc gacgtcaacg acaattcgtc ccatgttcag
      721 cgcctgcacg ttaacgctgt cagcttccgg cgtcagagtc agtttcattg tttcgcctcc
      781 ggatgcttcc cctgggtaat gttatttacc gttctgtaat tgtcgcgggt catcaggccg
      841 gtcgccctgc gagcccggag gatatcgatg ctgtttatta actgagaacg ggtacaggca
      901 ctgaattccg gctggtcggt acgcaccagt gcgtattttt caacgagaaa gttcaccgcg
      961 tcacacagtg aaatccctgc ctcaatatgc tgctcaatca cacgttcatc agcgaacggt
     1021 gtgtcattca gcgtgagacc atagtgctgg tccagcagtc gggtgagcag tgtctgccag
     1081 atggtgacgg gagacgggca gcccgatgcc tcccgtacgt gagtgtcggg taatgtattc
     1141 atcttaagtt ctctgtaaca ggtggtgata aaggggagat atccggggtt atacggtgat
     1201 tgcgggcgct gccggtgtcg gataaacagc cagataaacg taaccacagg agcctagggt
     1261 gtcggcttcg caggtcagcc ctttcgcata cagtgtgacg gtatgctgat ggcggggatt
     1321 cagttcaccg ccggtgagca tgagttccag ttgtttcatc agcagcggaa aggcctggtc
     1381 caggtggtac gcatccgcgt cgctgaacag gcctctgata ccggcgcggt cggcaaggta
     1441 atgcaggcgg ttaccctcct gcaccagacg tgccccgaaa cagggcgtca cggtgcaggg
     1501 cagcccccac taggggcggt cgtgattgtc gtcgggatgc gttgttccgg ggagtgtgtc
     1561 tgacacgata aagtcctcac agaaaatcag ttaagaatgg ttcgatggtg gcgataattc
     1621 tgctcatctg aatgccctga ttcagttcag ggtgacgctc atcagccgga catacgggcc
     1681 aaaactgtct ttacggcgtt ctgcaaatac agccagcaca ccgggaatat tctgcacttc
     1741 acgaccggta tacgcttcag tgctgccgtg ccagcggtat ttgccggtac agaacggaaa
     1801 aagacgggat gccggatgtt gttggtgaat acgcatggct tcaccacggg tgatgatttt
     1861 catagtgtga tacctctgaa gacagaagat aaaagcgaaa acaggtatga tgtggttgtg
     1921 acggtgacag gttaaagcag accgtgttcc gcaaaggaga aaacctggct gccaccgact
     1981 atcagatggt ccggcacccg gatgtcaacc agggccagcg cctgtaccag acgttcggtg
     2041 ataagccggt ctgccttact gggtgtgact tcaccggacg ggtgattgtg tgccagcacc
     2101 acggcagcgg cattgtggta cagggcgcgt ttaatcactt cccggggatg gacttccgtg
     2161 cggttgatgg tgccggtaaa gagggtttca ccggcaatca gctgattctg gttgttcaga
     2221 tacagcaccc ggaattcttc acgctccagt cccgccatgt tcagaatcag ccattcccgt
     2281 gccgctcggg tggaggtgaa ggccacgccg ggttcatgaa gatggcggtc cagggttttc
     2341 agggcccgca gaatgaggct gcgctcgccg ggcgtcatcg cgccgggcag aaaggaaagt
     2401 tgttgcattg tacttctcct gctcagtcga tgatgcgcat aatggcgctg cattccggat
     2461 gctgcagggc gtaatcccgc agccggtaat aatggaccgt catggcataa cactccgtac
     2521 gacaggcgtg atgactgtac gtcatcagac aggcggcaat gccggcggct tccgggctca
     2581 tttcagcgcg gttaccgttc atggcattga acagtaccca tgtttcgtca tcatcgtcat
     2641 ccggttcggg tgccataaat gccccgccgt tgttcagggt atacagattc cagataccac
     2701 cacagtagtc ttcgcaaaga cggtccatcc agccgaaaac acggggctcc agggtcaccc
     2761 actgtggaat gaggccaaaa tgctgcggcc agaagctgat gcgctgttca tcagggacta
     2821 tggtggcaac cagttgttgc aggccagtct ccggtgcaca ggctgaagag gtggtgttct
     2881 gagacaatgt tttcatgtgt gaaatccttg taatgtgaat aaatgagagg aaataaaggc
     2941 cgggggctga caacatcacg ttcatcactg accgtgagac ccggcaatat cagggatgaa
     3001 tgatattcgg ctgaaattcc ggacagccgt taaccgttcc ggtcacagac ggatggccag
     3061 atgccattgc ctgtactcag tctgcacaga ctgagttctc cgggacggta aagcagacgg
     3121 gtacaccagg cgcacaggtc atcatcagaa atggaacgac atcccggcag cgcctccggg
     3181 gcgtcgggtg atacgggtgc ctgaatcacg ccttcaggga tatgtttcat cagcgcatgc
     3241 tctccagcag cgtttctgcc atcacccaca atgcgcggtt gagcttaata tcggtgtcga
     3301 tgctgtgaat ggcacgggtg tggatacgtt ttccttttgc actgcgaccg gaaatcccgc
     3361 ctttcagcat attctcctgg atggtctgat atgcgctcca caggtcttta ccgtaatcct
     3421 cccgacgtcg tggcgtcaga atgtcggcgg tggtgacggg ctgatgttcg tcaccataac
     3481 ggtaagtcag tgccgcctgt gccagcgcct ggcgtgccgg tggcggcagg accagcgact
     3541 gcatggcatc acgtttctct tcaatccggt caaaaacgcc caccacctcg taagcccctt
     3601 caatgacttt ctgcaccaca tttccccggt gtggaacacg cacttccccc agagactgac
     3661 cacagacgca cccgttctgg cagacgaacc ggaagtaacc cggcagcatc tggtagctgg
     3721 aggtaccgtc atgggagttg agcagaataa tttcagggac atgttctccg tttatctctc
     3781 cggcccgccg cagacgcagc atgtgttttg tgtatccccg gcggcccggg tcgcgcacac
     3841 gggtctggca ggcgaaaaag ggctggaagc cttcccgctg cagactttcc agtacggtga
     3901 tggtggggat gtacgtatag ttctggcttc gggatgtgtg tttctcttcc ccgaaaatcc
     3961 ccggcacatg gtgcatcagc tcttcatgtg tcagcggacg gtcacggcgt atctggtttg
     4021 cagcataacc aaaacgactg actaatcgca tactttgctc cttatcggtg gttaagattt
     4081 actggtgcaa taaatgaaaa agccgcgtct cccggagaag acgcggcctg atggacgaaa
     4141 tcaatgacgt ttattgtctg agaagccctc cactggcgag ccacgcatta agctgttttc
     4201 cggcatcagc ttcagagttc cggcagcgtc agcgtcgctg accttccgcg ttgcggataa
     4261 cccgacggaa atgcattccc gggtcatcct ggataaacac attgcggtaa gcggttatga
     4321 cagcaaccgc ctgcccgtat gagaaagatc cttcagccag gacatactct gtgtgtaacc
     4381 cggcatatct ggtttctcct gataaatagc ctctgccata cgttgtggca gaggctgaag
     4441 catgaaactg acttcaggga tcagttaaca tttttccgga aacggtaatc agcagtggat
     4501 ggtagtcctg gggat
//
LOCUS       DAZGXZ010000247         4476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000247 DAZGXZ010000000
VERSION     DAZGXZ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.248"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1442
                     /locus_tag="ACHKJF_005222"
     CDS             <1..1442
                     /locus_tag="ACHKJF_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HFP0952415.1"
                     /translation="ALIIDEAQALHSSWLETLSCWFENKRIIAFCDETQTFSFEKGTN
                     YADLQRIINPQFSFNLSVVLRCPRAVTDYLIAVSPASYQITSPRNNEPDTLKELVVTD
                     PLSTLLNEILQLKESSVLPDEITVLVPTDLVKNSLQNDMRTLGVNIETVSRFRGMESS
                     VVITLYSESMEDAQLFCAYSRATTAFIAIYDSEKLAWREKNKFLHKLVNRKEVQRAIN
                     EAKHNSSSSVLMAGFFREGELKLSTVKLAWSKHFSSWLVEFDERNSPEEMWIDYLYSY
                     LPYPIIYWFRDSCRAVNYIKNKQFELNELGSGIHLVIKNCSICKKMVPFYSDDNCLLC
                     NKKNEELSPTRDEINKIREFDVTISQDNLNSVTQRLLPTPLAAVRARRAAFENPYRNK
                     VLSESLPAGNLLYRSALAFIQALIALVPSKKILDLDKITDALRRRYVHLEQLEYTELR
                     NALACALSTCYNRKYLIKVAKGKYLPVDD"
     gene            1524..1871
                     /locus_tag="ACHKJF_005223"
     CDS             1524..1871
                     /locus_tag="ACHKJF_005223"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952416.1"
                     /translation="MPGQPIPSELKEKGLIWLLPPYCWSHRQIARKLKISASVVSKWR
                     YELVEQGLLPENEKITSVDVEDWTPERRFSVVLETATLSEMELADYYRRMGLFVEQVK
                     EWRAISIQAHEKN"
     gene            2044..2208
                     /locus_tag="ACHKJF_005224"
     CDS             2044..2208
                     /locus_tag="ACHKJF_005224"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952417.1"
                     /translation="MERRKIIALVREAVSNGARKSQACRCIGISIRTLQRWTDGNVIT
                     TDVSVNGAPY"
     gene            complement(2212..2400)
                     /locus_tag="ACHKJF_005225"
                     /pseudo
     CDS             complement(2212..2400)
                     /locus_tag="ACHKJF_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            2461..3585
                     /locus_tag="ACHKJF_005226"
                     /pseudo
     CDS             2461..3585
                     /locus_tag="ACHKJF_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
     gene            complement(3582..>4476)
                     /locus_tag="ACHKJF_005227"
     CDS             complement(3582..>4476)
                     /locus_tag="ACHKJF_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFP0952418.1"
                     /translation="SVFCTGVAEKYALIEQWRQQFPIEAMCQVFGVSKSGYYNWVQHE
                     PSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELRLRC
                     KQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDV
                     YTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQF
                     GLKTSMSRKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEIFYNRQRR
                     HSRLGNISPAAFREKYHQMAA"
BASE COUNT         1259 a          946 c         1022 g         1249 t
ORIGIN      
        1 atgctttaat cattgatgaa gcacaggcgt tacattcttc gtggctggag actttatctt
       61 gttggttcga aaataaaagg attatcgcat tttgtgatga aactcaaaca ttttcctttg
      121 aaaaaggaac aaattacgct gatcttcagc gaataataaa tccccaattt tcttttaact
      181 tatctgttgt gttaagatgt cctagagccg ttactgatta tttaatagct gtcagccctg
      241 cctcatacca aattacttcc cctaggaata acgaaccgga tacgctaaaa gaattagttg
      301 ttacagatcc actctccaca ttgcttaatg aaattttaca attgaaagaa agttctgtac
      361 tgcctgatga gattactgtc cttgttccta cggatttagt gaaaaattcc ctccagaatg
      421 atatgagaac tttgggggtg aatattgaaa cagtatctcg ttttcgaggt atggaatcat
      481 ctgtcgtgat tacattgtat tccgaaagca tggaggatgc acagctattt tgtgcttatt
      541 caagagctac aactgctttt attgcaattt atgattcgga gaaattagca tggagagaaa
      601 aaaataaatt tttgcataaa ctggttaaca gaaaggaggt tcagagagca attaatgaag
      661 caaagcataa ttcttcctcc agtgttctta tggccggttt tttccgcgaa ggagagttaa
      721 aacttagtac tgtaaaatta gcatggtcga agcacttttc ttcgtggctt gttgaatttg
      781 atgaaagaaa ttctccagaa gaaatgtgga tagattattt gtattcctat ttgccttacc
      841 cgattatata ttggttcaga gattcatgta gagctgttaa ttacataaaa aataaacaat
      901 ttgaattaaa tgaattaggc tcagggatac atctggtaat aaaaaattgt agcatatgta
      961 aaaagatggt gcctttttat tctgatgata attgtttgct ttgtaataag aagaatgaag
     1021 agttgtcacc aaccagagat gaaattaata aaattcgcga gtttgatgta acaatatccc
     1081 aggacaatct taattcagtt actcaacgat tgttacccac accattagct gcggtaagag
     1141 cccgaagagc tgcatttgaa aatccatata gaaataaagt actaagtgaa tcattacctg
     1201 ctggaaacct tctttacaga agtgcattag cttttataca ggccttgatt gcattagtcc
     1261 catcaaagaa aatactggac ttggataaga taacagatgc tctgcgtagg cggtatgttc
     1321 atcttgagca attagaatat accgaattaa gaaatgcttt agcttgtgca ttgtcaactt
     1381 gttataatag aaaatatctc attaaagttg caaaaggaaa atatttacct gttgacgact
     1441 aggtgcgtat taccgtgaag gagatcggtg agtaaccccc tgtgtcagca tagttgtcgc
     1501 ctcaattttt taaggagata acaatgccag gacaaccaat accaagtgag ctcaaagaga
     1561 aaggactgat atggctttta ccaccatact gctggtcaca tcgtcagata gcccgtaagc
     1621 taaagatcag tgcctcagtt gtatcaaaat ggagatatga gttagtggag caagggcttt
     1681 tacctgaaaa tgaaaagatt acctccgttg atgttgaaga ctggacaccg gagcgtcgat
     1741 tctcagttgt tcttgagacg gctacgctgt cagaaatgga gcttgctgat tattaccgac
     1801 gcatgggatt gtttgttgaa caggttaaag aatggcgggc tatctcaatt caggcgcatg
     1861 aaaaaaattg acggaaaata ttcgaacaga cagggcattg cgtgacgcaa aaaataagtt
     1921 acgagaactg tagaaagagc ttaagcgtaa agataaagct ctggcggaag ctgcggccct
     1981 gcttatattg agggaaaagt tcaatgccct ctgggacaac agcgaggaag actgacccct
     2041 ctcctggagc ggcgtaaaat tattgccctt gtccgcgagg ccgtctccaa cggcgcccgg
     2101 aaaagtcagg cttgtaggtg tatcggtatt tcaattcgga cacttcagcg ctggactgat
     2161 ggcaacgtta ttaccacgga tgtaagcgtc aacggagcac cgtattgacg cttatttatt
     2221 ggtgagtact acgttccatg gcaggagttc atcaactcgg ttggaaggcc attccggcag
     2281 tacgctcagg ataaggcgca gatacgcttc cggatcgata ccgttcagac ggcaggtgcc
     2341 gatcagcccg tacaacagtg ctccacgctc gccgccgtga tcgctgccaa agaacataaa
     2401 gatggtatga cctttatata ctcactatag cattggtggg atattccaga ggaggtcatt
     2461 atgcattcgg aaaatattgc agcttacatc gggctggatg tacataaaga aacgttggcg
     2521 gttgctattg ctgcacctga acggcttggc gaagttcgtt attatggtac gattaacaac
     2581 gaagcccagg ctgtacgtcg attatttcag aaattgcagg gactttacgg aaacattctt
     2641 tcctgctatg aggctggccc ctgtggtttt gggctatatc atcaactcac agcgatgaat
     2701 ataaaatgcc aggttattgc cccttcccgg ataccaaaat caccgacaga cagaatcaaa
     2761 aatgatcatc gggacgcaat ttctcttgcc aggttactgc gggctggtga acttacccct
     2821 gtctggatcc ctgacctgac tcatgaagct atgcgggatc taatcagagc ccgtgcagca
     2881 gctaaacgag acagcagagt tgcccgacag cgaattctga gcatgctatt gcgaacagac
     2941 aaacgttatg cagggaagca ctggaccggc aaacatcgaa cctggttagc caatcaatca
     3001 tttagtcagc catctcaaca gattgctttt caacactatt gtcagagtct ggagcagatt
     3061 gaagatcgca tactttaact cgaccaggaa atatcccgcc tgttacctga gtggtctctg
     3121 tgcaatctgg tctgccagtt acaggccctt aaaggtgttg gtcagctaac cgcaataacc
     3181 ctggttgctg aactgggtga tttttcacga ttttccagtc ccaaacaact catggctttt
     3241 ctgggactgg ttccagggga atattccagt ggaaacagca ttcgtcccag gggaataaca
     3301 aaagttggaa acagcgaact gagacgtctg ctttacgaag ccgcctggtc ttatcgtaca
     3361 cctgcaaaag ttggtgcatg gcttatatat taccgaccgg attctgtaac acaatattcc
     3421 aaagatattg catggaaagc tcaacaacga ttgtgttctc gttaccgaag tctgacagca
     3481 aaagggaaaa aatcacaagt agccattacg gcggtggctc gtgagttgac tggatttatg
     3541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
     3601 tatttttccc tgaaggctgc cggggagata ttccccagac gagagtgacg acgctgacga
     3661 ttgtagaaaa tctcaatgta ttcccgtatt actgagatgg cttcatcccg gttattaaaa
     3721 cgatagtggc tcaggctctc atttttcagc gttccccaga agctttccat cggagcgttg
     3781 tcgtaacagt tacctttacg cgacattgat gttttcagac caaactgctc ctgtatgacc
     3841 cggtaatcgt atgcgcagta ctgtgaacct cgatcagagt ggtggattag cccggcaggt
     3901 gggcgctggc tcctgagcgc cataaacagg gctttacctg tcagctcttt tgtcatgcgc
     3961 tctcccatgg cgtagccgac aatttcgcac gtataaacat ctttgatgcc agcgaggtac
     4021 aaccatccct cctgtgtggc aacatacgtc aggtccgcca cccagacctg atttggtgct
     4081 gtaggagcga acgtctggtt cagcagattt ggcgcaactg gcagattgtg gttcgagttc
     4141 gtagtcgctc tgaacttgcg tttctgctta cagcgtagcc ttagctcctt acgaagacgt
     4201 gccagtcggt cacgaccaac gatgatgcca ttctctgcca gctccgtctg gagccgccgg
     4261 gttccatatg tttcgcgagt gcggatatgt gccaccttaa tctccagttt tagccgctca
     4321 tcactttgtt ttctgtctga gggttcatgc tgtacccagt tgtaataacc gctcttggat
     4381 acaccaaata cctgacacat cgcttcaatg ggaaattgtt gtcgccattg ttcgattaac
     4441 gcgtattttt cagcgactcc tgtgcaaaat acgctg
//
LOCUS       DAZGXZ010000248         4471 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000248 DAZGXZ010000000
VERSION     DAZGXZ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.249"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            164..1564
                     /gene="gabP"
                     /locus_tag="ACHKJF_005228"
     CDS             164..1564
                     /gene="gabP"
                     /locus_tag="ACHKJF_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP0952419.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPALMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            1585..2247
                     /gene="csiR"
                     /locus_tag="ACHKJF_005229"
     CDS             1585..2247
                     /gene="csiR"
                     /locus_tag="ACHKJF_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP0952420.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(2248..2697)
                     /gene="kbp"
                     /locus_tag="ACHKJF_005230"
     CDS             complement(2248..2697)
                     /gene="kbp"
                     /locus_tag="ACHKJF_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP0952421.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(2781..2939)
                     /locus_tag="ACHKJF_005231"
     CDS             complement(2781..2939)
                     /locus_tag="ACHKJF_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0952422.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            3122..3421
                     /locus_tag="ACHKJF_005232"
     CDS             3122..3421
                     /locus_tag="ACHKJF_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0952423.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            3431..3955
                     /gene="ygaP"
                     /locus_tag="ACHKJF_005233"
     CDS             3431..3955
                     /gene="ygaP"
                     /locus_tag="ACHKJF_005233"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP0952424.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPAKLRHEQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPFPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(4002..4406)
                     /gene="stpA"
                     /locus_tag="ACHKJF_005234"
     CDS             complement(4002..4406)
                     /gene="stpA"
                     /locus_tag="ACHKJF_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP0952425.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         1039 a         1097 c         1132 g         1203 t
ORIGIN      
        1 cacgcggcta caccctcacc ccggccctct ccctaaggga gagggagaaa accctcccgc
       61 tttcgttttt aagattttgt tgaccgtctc aattaaacgc gtgacccggc gcgggaaatg
      121 tcggggcgct ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca
      181 accacatgag ttaggcggcg ggctgaagtc acgccacgtc accatgttgt ctattgccgg
      241 tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag cgggcccggc
      301 ggtattactg gcctatctgt tcgccggatt actggtggtt atgattatgc gaatgctggc
      361 ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc acctatgccg ataaagccat
      421 tggccgctgg gcgggttata ccatcggctg gctgtactgg tggttttggg tactggttat
      481 cccgctggaa gccaacatcg ccgctatgat cctgcactcg tgggttccag gcattcccat
      541 ctggttattt tccctcgtca ttaccctcgc cttaactggc agtaatttat taagcgttaa
      601 aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt
      661 tattttcctt ggtgcagtcg caattagcgg tttttaccct tatgccgaag tgagcgggat
      721 ctcaagattg tgggatagcg gcggctttat gcccaacggc ttcggtgcgg tattaagcgc
      781 gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga
      841 atccgacacg ccggaaaaac atattgtccg cgctaccaac tcggttatct ggcgtatttc
      901 tatcttctat ttgtgctcta tttttgtcgt agtggcgtta ataccgtgga atatgccggg
      961 gctgaaagcc gttggttctt atcgctcggt tctggaattg ctcaatattc cccatgcgaa
     1021 attaatcatg gactgcgtga tattactttc cgtaaccagc tgtctgaact cggcgctgta
     1081 taccgcgtca aggatgctct actccttaag ccgtcgcggt gatgcgcccg cgctaatggg
     1141 caaaatcaac cgcagtaaaa ccccgtatgt ggcggtgtta ctctccaccg gagcggcatt
     1201 tttaacggtg gtggtgaact attacgcacc tgcgaaagtg tttaaattcc tgatagacag
     1261 ctccggcgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt tgcggatgcg
     1321 caaaattctg cgagcagaag gaagcgaaat tcgcttgcgc atgtggcttt atccctggct
     1381 cacctggctg gtcatcggct ttattacctt tgtgttggta gtgatgctat tccgtccggc
     1441 gcaacagtta gaagtgatct ccaccggctt attagcgata gggattatct gtaccgtgcc
     1501 aattatggct cgctggaaaa agctggtatt gtggcaaaaa acacccgttc ataatacgcg
     1561 ctgataatga tcaggagtca caccatgacc attacgtctc tggatggcta tcgctggctg
     1621 aagaacgata ttattcgcgg taattttcaa ccggatgaaa aattacggat gagtttgctg
     1681 acatcgcgtt atgcacttgg cgttgggccg ttgcgggaag cactttctca actggtggcg
     1741 gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag
     1801 gagctgctcg atattttcga cgcccgcgcc aatatggaag cgatgctggt cagtctggcg
     1861 attgcccgcg gtggcgatga atgggaggca gacgttctcg caaaagcgca tctgctcagt
     1921 aagctggaag cctgtgacgc cagcgagaaa atgcttgatg agtgggatct gcgtcatcag
     1981 gcgtttcata cagcaattgt ggcgggctgt ggttctcact atttgctgca aatgcgtgaa
     2041 cggttgtttg atctggcggc gcgctatcga tttatctggc tgcggcgaac ggtgctttcg
     2101 gtagaaatgc tggaggataa acacgatcag caccagaccc tgactgcggc ggtactggcg
     2161 cgagataccg cgcgcgccag tgagttaatg cgccagcatt tactgacgcc aattcccatt
     2221 atccagcagg cgatggctgg caattaacta ctcttccgga atacgcaaca cttgccccgg
     2281 ataaatttta tccgggcttt ttagcatcgg tttattcgct tcgaagattt tattgtacag
     2341 attagcgtta ccgtagacct gtttggaaat ggcactcaga gtgtcgccag acttaacggt
     2401 ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcga cactggcaat
     2461 accggaaata ttcccaaccg caacaaggat tttctccttc gcctcctgac tcaggccgtc
     2521 accagtgacc gtcgctttgc cgtcggcaat ttgaatgttc actttatcgg catcaggtat
     2581 accggttttg ctcagatgct cctgcacctt cttcgcctga tcgtctttat cgtgctgacc
     2641 tgtaaccgcg tcccagagtt tttctccggc atctttcaca aaattgaaca gacccatagc
     2701 tacctcattc gttaacggaa acactcagaa agtgtagcag aggaacgcgg cgcgttaacg
     2761 ccgcggggat gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg
     2821 gggatatacc caagcagtgt caacagaata ttaataatga acgcccaacc gaacccttta
     2881 ccgagcagca cgccgagcgg cggcagaatg atggtgatga cgattctcca gaaacccata
     2941 tgtactccct ataagaaaat tactcattgt ttaaaaagag attttatctc ttaagcgtag
     3001 taattttagc ggagcctgcc agtttcagct tacgctttta ctctcattta cgctgctgag
     3061 tctggtatat gttgcatttt tagtttagag tttacttaat ttagaaaata cttaaataaa
     3121 tatgactgaa ctcgcgcaat tacaggccag tgccgaacag gcagcggcct tattgaaagc
     3181 aatgagccac cctaaacgat tgctgattct gtgcatgctt agcggttccc ccggcaccag
     3241 cgcgggagag ctgacgcgca ttaccggact gagtgcctct gcgacatcac agcatctcgc
     3301 tcgtatgcgg gacgaagggc ttatcgacag ccaacgggat gcccaacgca ttctatattc
     3361 cattaaaaat gaggcggtaa atgccattat cgccaccctg aaaaatgtct attgtccgta
     3421 aggagtcact atggctttga caaccatttc gccgcatgat gcacaagaat taatcgcacg
     3481 cggcgcaaag ttaatcgata ttcgtgatgc tgatgaatat cttcgtgaac atattcctga
     3541 agcagatctg gctccattat ccgtgctgga acagtcaggt cttccggcta aattacgtca
     3601 cgagcaaatt atattccact gccaggcagg taaacgcacc agtaataatg ccgataaatt
     3661 agcagcgatt gccgcccccg cagaaatatt tttactcgaa gatgggattg atggctggaa
     3721 aagagctgga ttgccagtag cggtaaataa atctcaaccc tttccgttaa tgcgccaggt
     3781 gcagatcgct gcgggtggtt taatattaat cggcgttgta ctgggttata ccgtaaatag
     3841 cggtttcttc ttattaagtg gctttgttgg tgccgggtta ctgtttgcag gaatcagcgg
     3901 tttttgtgga atggcaaggt tgttagataa gatgccgtgg aaccaacgag cttgagaagc
     3961 gacgccggac gcgccctagc agcgacatcc ggcctcagta attagatcag gaaatcgtcg
     4021 agagatttac cttctgccag cgcctgagca attggcttcg gtgtacggcc ctgaccggtc
     4081 caggttttag tttcaccgtt aacatcggtg aatttatatt tcgccggacg cggctggcgt
     4141 tttttaccag cgcgtggtgc agcagcagag ctattaccta ataactcttc cgggttaatt
     4201 ccgtcagcct tcatcagctc cagccaggtg ctaatttttt cctgacgctc agccagttca
     4261 cgctgctgct gttcttcttc ttcacgtctt tctttagtga caaccctgaa tttttcgagc
     4321 atttcttcaa gaacgtcaat ggagaattcg cgagccatcg cacggagagt gcgaatgtta
     4381 tttaaacttt gtaacattac ggacataata aagaaaacct tttaacgcca aaacaaaaaa
     4441 gtatttcgtc gcgtagtgta agcgatcact t
//
LOCUS       DAZGXZ010000249         4422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000249 DAZGXZ010000000
VERSION     DAZGXZ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.250"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..1378
                     /locus_tag="ACHKJF_005235"
                     /pseudo
     CDS             1..1378
                     /locus_tag="ACHKJF_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            1433..1747
                     /locus_tag="ACHKJF_005236"
                     /pseudo
     CDS             1433..1747
                     /locus_tag="ACHKJF_005236"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            1956..2222
                     /gene="hipB"
                     /locus_tag="ACHKJF_005237"
     CDS             1956..2222
                     /gene="hipB"
                     /locus_tag="ACHKJF_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP0952426.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            2222..3544
                     /gene="hipA"
                     /locus_tag="ACHKJF_005238"
     CDS             2222..3544
                     /gene="hipA"
                     /locus_tag="ACHKJF_005238"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP0952427.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            3856..4064
                     /locus_tag="ACHKJF_005239"
                     /pseudo
     CDS             3856..4064
                     /locus_tag="ACHKJF_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1260 a          907 c         1128 g         1127 t
ORIGIN      
        1 cggtggcaat gccaccggtg tcggaaccct gaatgtgcag gatcaagact ctgtcatcac
       61 ggtacgcaga ctctataatg gatatttcgg taatggcgca gtcaatattt ccaataatgg
      121 actaattaat aacaaagaat attcattggt gggcgttgaa gacggttccc acggtgtcgt
      181 caacgtgacc gataaagggc attggagttt cctcggaaca ggcgaagctt tccgctatat
      241 ctatatcggt gatgctggtg acggtgaact taatgtctcg cgtgaaggta aagtagattc
      301 gggaattatc actgcgggga tgaaagaaac aggcacaggc aacattactg ttaaggataa
      361 gaactccgtt atcactaatc tcggaactaa tcttggttat gacggccacg gcgaaatgaa
      421 tatcagtaat gaggggcttg ttgtcagcaa cggaggaagc tcactcggtt atggagaaaa
      481 cggcgtcggg aatgtcagca tcaccacggg ggggatgtgg gaggtcaata agaatgtcta
      541 taccaccatt ggtgttgcgg gcgtcggaaa cctcaatatt agcgatggcg gtaagtttgt
      601 atcgcaaaat attacttttt tgggcgataa agcaagcggt atcggcacac tgaacctgat
      661 ggatgggaca tcatcgttcg atactgtggg tatcaatgtc ggtaattttg gtagcggtat
      721 cgtaaatgtc agtaatggtg ccacccttaa ttcaacgggc tatggattta tcggaggaaa
      781 tgcctccggt aaagggatag ttaatatttc aacggatagt ctctggaatt taaagacatc
      841 atctactaac gcccaattgc tacaggtcgg tgtattaggc acgggtgaac tgaatattac
      901 caccggaggt atagttaaag cgcgtgatac acagatagct ctcaatgaca aaagtaaggg
      961 cgacgtgagg gtggatgggc agaactctct tcttgaaaca ttcaatatgt acgtagggac
     1021 atctggtacg ggtacgttaa ccctgacgaa tagcggcacg ctgaatgtcg aaggtggaga
     1081 agtttactta ggtgtttttg aacctgctgt aggaacgcta aacattggtg cagctcacgg
     1141 tgaggcggcg gcagatgccg ggtatataac caacgcgacg aaagtagagt ttggttctgg
     1201 cgaaggtgtt tttgtcttta atcatactaa taacagtgat gccggctacc aggtcgatat
     1261 gctgattacg ggtgacgata aagacggaaa agtgatccat gatgcaggcc atacggtgtt
     1321 caatgcaggg aatacttata gcggtaaaac gctggtcaat gacggcctcc tgaccatggg
     1381 tgtgaaagat tctgaccata cccggaaaga cggaacacgc attgaaacgg aaggcgacgg
     1441 aaacgtgcaa acgcgacttg gggtgaaaac ctacctgaac agccatcacc agcgtgacga
     1501 tggtaaacag cgtgagttcc agccttacat tgaagcgaac tggatcaaca atagcaaagt
     1561 ctacgccgtg aagatgaatg gtcaaaccgt aagccgtgat ggtgcgcgta atctcggtga
     1621 agtacgtacc ggggttgagg cgaaagtaaa taacaacctt agcctgtggg ggaatgtcgg
     1681 tgtgcaacta ggtgataaag gctatagcga tactcagggc atgctgggag tgaaatatag
     1741 ctggtaaacc gtataagccg catgtcgaga tggcatgcgg cttaatattg ccgacttcaa
     1801 aggttttaaa gcctcccttt tatccgtgat cgcggatatc gcagcgttta tcccgtacag
     1861 cggataagat gtgtttccag attgacttta tcctcactaa aggataaaac tgataatatc
     1921 cccttaagcg gataaatttg ctgtggacgt atgacatgat gagctttcag aagatctata
     1981 gcccaacgca attagcgaat gcaatgaaac tggttcgcca gcaaaatggc tggacgcaga
     2041 gcgagctggc gaaaaaaatt ggcattaagc aggcgacaat ttccaatttc gaaaacaacc
     2101 ctgacaatac cacgctcacg acatttttta agattttaca gtcgcttgaa ctctcaatga
     2161 cactatgcga cacgaaaaat gcctcgccag aatcaacaga acagcaagat ctggagtggt
     2221 aatgcctaaa cttgtcactt ggatgaacaa ccagcgggta ggcgagttaa cgaagttagc
     2281 caacggcgcg cacaccttta agtatgcacc ggagtggtta gccagccgtt atgccagacc
     2341 gttgtcactt tcgctgccat tgcagagggg gaatatcacc tcagatgccg tatttaactt
     2401 cttcgataac ctgttacccg atagcccgat tgtacgtgac cggatcgtta aacgttatca
     2461 tgctaaatcc agacaaccgt ttgatttatt gtcagaaata gggcgagaca gcgttggtgc
     2521 cgtaacgtta ttacccgaaa atgaaaccat aacgcgcccg ataatggcat gggaaaagct
     2581 tactgaagcc aggcttgaag aagtattaac ggcttataaa gcagatatcc ctctgggcat
     2641 gattagagaa gaaaatgact ttcgcatctc ggttgctggc gcgcaggaga agacagcgct
     2701 gcttagaata ggcaatgact ggtgcattcc gaaaggaata acgccgacga cgcacatcat
     2761 taaattaccg attggcgaaa tcaggcagcc caatgcgacg ctcgatctca gccaaagcgt
     2821 tgataatgag tattactgtc tgctgctggc gaaagaactt gggttgaatg ttccggacgc
     2881 agaaatcatt aaagcgggaa gggtgcgcgc gttagcggtc gaacgttttg acaggcgttg
     2941 gaatactgag cgaacggttt tacttcgctt gccacaggag gatatgtgtc agacattcgg
     3001 tttaccttca tctgtgaaat atgaatcaga tggaggccca ggcatcgcgc ggatcatggc
     3061 gtttttgatg gggtccagcg aggcgcttcg cgatcgttat gattttatga aattccaggt
     3121 cttccagtgg ttgattggcg caacggatgg ccatgcaaaa aacttctccg tatttattca
     3181 ggctggcggc agttatcgac tcacgccatt ttacgacatc atttcagcat ttccggtcct
     3241 tggcggtacg ggaatacaca tcagcgatct caaactggca atggggctta acgcatccaa
     3301 aggcaaaaaa acggcaatcg ataaaattta tccgcgacat tttttggcga cagcaaaggt
     3361 gctgagattc ccggaagtgc agatgcatga aatcctaagt gactttgcca gaatgattcc
     3421 ggcagcactg gataacgtga agacttcatt accgacagat tttccggaga acgtggtgac
     3481 ggcagttgaa accaatgtgt tgaggttgca tggacggtta agccgagaat acggtagtaa
     3541 gtgagatatg gggtttggtc gttgttaatg agcacgacaa tcatgaccgc caacgactgg
     3601 attcttcctt ggtttatgct ggtgcgcata atgtatatta tgttaaatca tgtctgagtg
     3661 atgcgaagtg cttttctggt agtcgttatt cgttcagggt tcaagaactt aagcctctcc
     3721 tctttcataa ttggcctgtg catggagaaa tagcctggtt attccctgcc ccatgctaag
     3781 agcgcagtgt ctgtcgcata gacactgcat acgaaaacac cagagggtgc tgttacttat
     3841 ttaaggacgg atcaagcggg aatgagtcca ctccggtatc agaaatggct acgtctcaat
     3901 gaagtcaggc gaccgatgct gaatgaacat tacgatgtca ccacagcagc ctatgctgtc
     3961 ggttacgaaa gctatcccat ttcagtcggg aatattcgcg gatgtttgga gagtcaccca
     4021 agagagatat taccaggtta agaaagtctg tcggtcagct ttaataaacc ctgcacttat
     4081 ctgtttagag caattcggtg ttagtttcag caagcaaaca ttaaccatag ctaatgattt
     4141 atagccatat taaccattgg tagcccttga cctttcctga cacttccctt gttcgcaata
     4201 atattctgtt tagtcacggt ttataaataa catgcataat gaaatcgtca tgttatttag
     4261 cgacataaaa tgtttttgca aatactctct tcaacaagtg cacaataaac ttgttgtgat
     4321 taggtgtcct tgtgatttaa agatattatc gttcttcgtg aaatgtaata accctggaaa
     4381 ctatattctc agggcaacaa ccaatcaaga atgattcttg tt
//
LOCUS       DAZGXZ010000250         4421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000250 DAZGXZ010000000
VERSION     DAZGXZ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.251"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            25..597
                     /gene="lpfA-O113"
                     /locus_tag="ACHKJF_005240"
     CDS             25..597
                     /gene="lpfA-O113"
                     /locus_tag="ACHKJF_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP0952428.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            645..1376
                     /gene="lpfB-O113"
                     /locus_tag="ACHKJF_005241"
     CDS             645..1376
                     /gene="lpfB-O113"
                     /locus_tag="ACHKJF_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFP0952429.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1401..3923
                     /gene="lpfC-O113"
                     /locus_tag="ACHKJF_005242"
     CDS             1401..3923
                     /gene="lpfC-O113"
                     /locus_tag="ACHKJF_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP0952430.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGIGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3934..>4421
                     /gene="lpfD-O113"
                     /locus_tag="ACHKJF_005243"
     CDS             3934..>4421
                     /gene="lpfD-O113"
                     /locus_tag="ACHKJF_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFP0952431.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IV"
BASE COUNT         1215 a          941 c         1063 g         1202 t
ORIGIN      
        1 atatattata tcaatggagt ttttatgaag cgtaatatta taggcggtgc attcactctg
       61 gcatctctaa tgctggccgg gcatgcactg gcagaagatg gtgttgttca cttcgtcggt
      121 gaaattgtcg acactacttg tgaagttacc tccgatacag ccgatcaaat tgtcccactg
      181 ggtaaagtca gtaaaaatgc attttcaggt gtaggtagtc tggcgtcgcc acagaagttc
      241 agtattaagc ttgaaaattg cccggcaacg tacactcaag cagccgttcg ttttgatggt
      301 acagaagcgc ctggcggcga cggcgacctg aaagtgggta cgccgcttac agcaggcaac
      361 cctggtgatt ttaccggtac aggacaagcg attgcggcaa ccggcgttgg tattcgtatt
      421 tttaaccagt ccgataattc gcaggttaaa ctttataacg actctgctta taccgctatc
      481 gatgctgaag gcaaggctga aatgaagttt attgcacgct atgtggcaac caatgcgacc
      541 gtaacggctg gtacggcgaa cgctgattca caatttactg tcgaatataa gaaataattt
      601 atcaagggcc tttttattca gttggctttg gccagggagt cgttatgtcc agaaaaagtt
      661 gtttaatcag gctctttaaa acaacagttt catgtaagtt atttaccgcg attatactca
      721 gcgttttttt aggccaacca gcattaactt atgcaggcgt tgtcattggt ggtacgcggg
      781 tggtttactt aagtaataat gctgataaat ctatttctgt atttagcaaa gaagaaaaaa
      841 taccttatct catccaggct tgggtagatc catttaataa agaggataaa agcaaagcgc
      901 cgtttaccgt tattccgcca gtatcacgat tagaaccttc gcaagaaaaa atattacgca
      961 ttattcacac caaaggtgtg tcattgcctg atgatcgcga gtcagtcttc tggctgaata
     1021 ttaaaaatat tccgccatca gcgagcaaca aagcgacgaa ctcgctggaa atagcagtaa
     1081 aaacgcgtat caagctgttc tggcgaccag caaatatccg ccttattccc gaagatgctg
     1141 cgccgaaagt gaaatggcgc cgggaagggc gaaatctgat agcagagaat cccaacccaa
     1201 ttcatatcag tgtgatggat gtcatcgttg atggccatga tgtgcccctc aatatgatcc
     1261 gtccttttga aacgctcacg ttacctttac ctgctaattc tgcgggaggc caaatgacct
     1321 ggcgatttat taatgattac ggcgcagtca gtgacccgat aaaaatgacg ctgtaagcaa
     1381 gggataagga taggcgtatt atgatgacga ccagaatagt ggttggcctc acggcaggga
     1441 cgtgtctgat tttctcgcaa aacctgatgg ccgaggtcag tgtattcaat ccggcgcttc
     1501 tggaaatcga ccatcaatcc ggagtcgata ttcgccagtt taatcgggca aacctgatgc
     1561 ccccaggtgt ttatagcgtt gatattttta tcaacggtaa aatgtttgaa cgtcaggatg
     1621 tgacatttgt tcaggataat ccagatgctg atctgcacgc atgctttatt gccattaaaa
     1681 aaacactgtc ctcctttggc ataaaagttg atgcgctcaa atcgttcaat gatgtggatg
     1741 agacggtttg cctcgatcct gctccacgta ttgaaggctc atcctggcag tttgacagtg
     1801 ataaattgca gctgaatata tccattcccc aaatctacat ggacgcgatg gcttatgatt
     1861 acatcagccc cacgcgttgg gatgagggga ttaacgcgct caccatcaac tacgattttt
     1921 ctggttcaca tacactacgt tcagattatg gttcacaaga gacagatacc agttatctca
     1981 atctgcgcaa tggactgaat attggaccgt ggcggctacg taattacagt actttaaaca
     2041 ccagcgatgg ccgtgcggaa tacaactcca ttagtacctg gatacagcgc gatattgccg
     2101 cgttaagaag ccagattatg attggtgata cgtggacggc gagcgatatt ttcgacagta
     2161 cgcaaattcg cggcgcgcgt ttgtatactg ataacgatat gctacccgcc agccagaatg
     2221 gctttgctcc tgtggttcgt gggattgcaa agtccaacgc caccgtcatc attcggcaga
     2281 atggctacgt gatttatcag tctgccgttc cacaaggtgc ttttgagatc accgatctca
     2341 acaccgccag tacaggtggc gatttggacg taaccatcaa agaagaagac ggtagcgaac
     2401 aacgattcac ccaaccttat gcttcattgg cgattcttaa acgtgaaggt cagacagatg
     2461 ttgatgtcag cgtgggtgaa ttgcgcgatg aagacggatt tacaccggac gtccttcagg
     2521 cgcaaatact tcatggtttt tcccacggga tcactttata tggaggtatg caggctgctg
     2581 aaaattatgg ttctgcagct ctgggtatcg gtaaagatct tggcgctttg ggcgcaattt
     2641 ctttcgatgt gacacatgct cgtgcgaatt ttagccatga tgatacagaa acgggtcagt
     2701 catatcgctt tctctattca aaacgatttg acgacacaga cactagcttg cgcctggttg
     2761 gctatcgtta ctccaccgag ggctactata ccctcaatga gtgggcatcg cggcgcaaca
     2821 gccctgaaga cttttgggaa acaggtaacc gacgtagtcg cgtggaggga acgctaacgc
     2881 agtcgttggg gagagattat ggcaatttat acctgacatt aagccggcaa caatactggc
     2941 ataccgatga tgtcgaacga ttaatgcaat ttggctacag cagtagctgg aagcgtctct
     3001 cgtggaacgt ctcctggagt tattccaata ctgccagaca ggggacgggg aacaaccatg
     3061 ccagtgataa caccagtgag cagatctaca tgctctcttt atctgttcct ttatcgggct
     3121 ggtggggtaa tagttacgcc acctattctg tttcgcaaaa cgataattcc ggtagctcac
     3181 atcaactcgg actcagcggt acggcgctgg aaagaaataa cctttcatgg aatttaatgc
     3241 agtcctataa cagtcatgat gatgaggttg gcggtaatat gtccctgacc tatgatggct
     3301 cttatggcac ggtgaacggc agctataact acagccaaaa ttcccagagg ctgaattatg
     3361 gtatcagagg gggaattctg gcacacagcg aaggggtaac gttaagtcag gagttaggtg
     3421 aaactattgc tcttgttaaa gcacctgggg ccgccgggtt agaaatagat aatatgcgcg
     3481 gtgctgcgac ggactggcgc ggctatacgg tcaagacaca gctaaaccct tatgatgaaa
     3541 atcgggtagc aatcagcgat aactatttct cgaagtcgaa tatagaactt gataataccg
     3601 tcgttacgat ggttcccacg cgtggtgcag tggttaaagc ggagtttgtg actcatgtgg
     3661 gttatcgcgt tctcttcaga gtgttaaatg caaatggtaa accggtacct tttggagcca
     3721 ttgctgcgat acaagatgca agtttggcag attcaggaat tgtcggtgac cgtggcgaac
     3781 tttatctttc tggtctacca gaaaaaggac aggttacgtt atcctgggga gaaaacgcct
     3841 caacaaaatg catcttcaat tattcacttt cgacaccaga aagtgagagc ggattaattg
     3901 aacagggtgt gacatgtcat taaaaggatt tttatgaaca agtatatcaa acagtggtgc
     3961 tttgctgtgt ttatgctctc gttaagtagt gtagcacttg cagctcctaa aggtatctgt
     4021 acccctgata atggcgtatt tcacagtacg cttgatttct cgggatatct tattaccgcg
     4081 aacgaaaata aggttggaac gacatttaac accactgtaa ctaatggatc ctcatatcct
     4141 ggacgctgtc attgtgatac tggtaacgta ggagaattcc cctatatcta ttatacgtca
     4201 aaaataaacc aggcattaac ttatgctggg gttcactcta atattaatta ttatgatctt
     4261 aatccgaacc ttgatgttgg gattgctata gatattcttg gggttgggta tgtgaatgca
     4321 ccttttgaat atcatgctaa caacccgtct ggtaatacaa agtacaattg caaccgcatt
     4381 gaacctttaa gtatatctag tggggctaaa gcgatagtat a
//
LOCUS       DAZGXZ010000251         4383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000251 DAZGXZ010000000
VERSION     DAZGXZ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.252"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..720
                     /locus_tag="ACHKJF_005244"
     CDS             <1..720
                     /locus_tag="ACHKJF_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0952432.1"
                     /translation="HVQVGADKKHELDGLDLFTGVTMTYTDSNAGSGTFSGKTKSVGA
                     GLYASALFDSGAYIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTE
                     DAWIEPQAELVYGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKV
                     TARAGLGYQFDLLANGETVLRDASGEKRIKGGKDGRMLMSVGLNAEVRDNIRFGLEFE
                     KSAFGKYNVDNAVNASFRYSF"
     gene            complement(1289..3961)
                     /gene="rcsD"
                     /locus_tag="ACHKJF_005245"
     CDS             complement(1289..3961)
                     /gene="rcsD"
                     /locus_tag="ACHKJF_005245"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP0952433.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         1117 a          998 c         1095 g         1173 t
ORIGIN      
        1 cacgttcagg tgggtgcgga taaaaaacac gaactggacg gtctggacct cttcaccggg
       61 gtgaccatga catataccga cagcaacgcc ggcagcggca ccttcagtgg taaaacgaag
      121 tcagtgggtg cgggtctgta tgcttcagcc ctgtttgatt ccggggccta tatcgacctg
      181 attggtaagt atgttcacca tgataatgag tatacggcgt cctttgccgg actcggcaca
      241 aaagactaca gctctcattc ctggtatgcc ggtgctgaag tgggttaccg ctatcatgtc
      301 acagaggatg cctggattga gccacaggcg gaactggtct atggtgctgt atccggtaaa
      361 cagttcagct ggaaggacca gggaatgagc ctgtctatga aggacaagga ctacaatccg
      421 ctgattggcc gtactggtgt ggatgtgggt aaatcgttct ccggtaagga ctggaaagtg
      481 acggcacgtg ctggtcttgg ctatcagttt gacctgctgg cgaacggaga aacggtactg
      541 cgtgatgcat ccggagagaa gcgtattaag ggcgggaaag acggtcgcat gctgatgtct
      601 gtgggtctga acgcagaggt tcgtgacaac atccgttttg gtctggagtt tgagaaatca
      661 gcctttggta agtacaacgt tgataatgct gtcaacgcca gcttccgtta ttcattctga
      721 cggttgttac tgaaggggat tatggcctga cggctccctg taaataaaga caggcacaga
      781 agattgtgag ttacttagtg ttccaccaaa aagtaaggtc gcaactctgt gcctgcttcg
      841 taagtgtaca gtgagggcca tatggtcata actgaagcat ccgacgacgt ggtgtctagc
      901 agagtgtcag atatgcagga aaatatgagc tccgacagaa atgcagtaaa aaaagggagt
      961 ctcgcactcc cctccagaaa actccagggc tgtaagttgt attaaaaagc tgatttttct
     1021 ttagcgatac agacctcaat aagtataaca cccttcagtt actgtggtta tgcacaggtg
     1081 ttatttttaa ctgtgtggat aaacagtgtc gattattcag attggttatg gggcatgtat
     1141 gggacatttt taatcggcga aatacgtcga agttcgtcga tatggtaaac gaatcatcta
     1201 tccaaccctt gaaaaacggc gctcctggac gatcttcgtc gatttttaaa atgcaacaag
     1261 tacgttgttc atgtaatagg ctaccttgct acagcaagct cttgacataa ctgtcaatgt
     1321 cgctgatgta tttttctatt cctggaacat ccttctcacg aatcagatgt tccagcgttt
     1381 cacataactg cttgccgggt accagattta gcatggcaaa tacgccttta agacgatgag
     1441 ccgtttgggc taacgcagca aagtcactgg ttgctgcttc agtatacagc ctcttaacat
     1501 catccggtac tgtgtctaca aagagcgcat aatagccgct ggcatggagt tgcgcatttt
     1561 catctccgcc cagaggcgat tctgtcacct cttcctgcgc cagttgcact tcaattaatt
     1621 gtaagaccgc ttcctgcata gcgttgctca tattgaagtt gacgcacaat tgaccaggcc
     1681 caatttcccg tacgccagac tcatcatcgc ttaaaagcaa gccagaggca gtaagattag
     1741 acggattatc cgttaaaaag atatcataat cttgactaat taatctttca tcgggtgtga
     1801 tacaggttgc accccaattt tctaactggc gagtgacaat attccgaatt tctgccgaag
     1861 taacatccac cattacgcag acatcatcca gtaaacgctc ttcttcctct tcaacttccg
     1921 ggtcagcggc gagcattttg atatgcacag agtagcgtgt accaagccca tcccgcgttt
     1981 tgatgtttaa atgaccgccc agtttacgcg ccagttgatc gctcagccag aatgccagcg
     2041 ggtccgcctt gccatagcga tcgttttggg tctggttgat aaacgggaag tgcaaattat
     2101 ccatttcatg aatacttacg ccttctccgg tgtccagaat gcggaatgtc aggcggtctt
     2161 cagaggactc atcctgatca acctcaaggg tgatttttcc caattgcgtt gaggtcacgg
     2221 catattgcat cagtagcagc aaaatacgtc gtaaggcatc gcgatcgccg cggcgcatat
     2281 cgtgtgcttt cagatgattg ttaatcagca gttgcagacc tttacgcttg atggcaggca
     2341 acactgaagg cacaacttca tcaattaaat cctgcacgga gaacagcacc gtctcacttt
     2401 tccagctatc atccgcaagc atgttcgcta actgtatttc atcgaccagc cgcaccagca
     2461 catctgcctg attcgccagt tgtttgcttt ccggggcgtt gagtttagcc gcgctctccg
     2521 ccagggactg tgcgggttct ttcagcgcat cgccaatgtt tttcataaag gtcatccggc
     2581 cctgctggtt tttctcatac agacgctgcg cctgcttgag tttcttgttt accagcactt
     2641 cgcgatcctg atcgcgaata atgaaaattt gtgtgcgcgg cgcgacctgg ctgcggaaca
     2701 tgcggatctc atacagctcg ttattgatcg tcgcctgaat aatcccctga tgctgttccg
     2761 ccatggtggt gatgttttgc agattcaaat gcggcagcaa atgatcggca attttgttac
     2821 ttatgacagt gcggttcgat tcctgatcgt gaaccagcag gccgagcggc agcagtgaga
     2881 ctatctcttc attgattgcc cgtaaaatgc gcaattcgtt attgaccgcc gtgttgggta
     2941 gactttctgt actgcgactg gagaaatggc ggaatgtggt atagccaaat aacgccagcg
     3001 ccagcaaacc gatgttcagc agcagtggca gcagaatgtt ttgcaacgta tccagcaata
     3061 aggtgccata aggaacctgc cagaccaggc gcatatcggt agagttgagt gccgaggaga
     3121 tttcaatctt cgtactgtta aagtggatac tgacgctatc cgttccttct ttctcattat
     3181 cattgtctcc cgttgccgtc gtgtctggct caaggcggaa actgtccagc ggcatacccg
     3241 gtgggatcaa atcattaatc ggcagatcaa aagccacgac cgttgccaga tgtcctggct
     3301 ggttaaaggt agtacgcaag gtaaagtaat gaccgttctg ccaggccagg cggcgcatgt
     3361 tagaaaagct ttcgcgttca tcgagggcgt tggcctgttg caacatctct gcacgacgtg
     3421 aatcaacaat gtcgctgacg gtcgattctt taaatccgga ggtgagatct ttaaggggta
     3481 gggttgagat cagcaccaga ctgttatcct gaccattcag gtaatacatc gaccacggta
     3541 cattttctgc gccccacaat gtatccagat aagtggacat ccgctgagtc atctcaagcg
     3601 ttgagttgtc gtgagagcca aagattaacg cttccgtttt gcggcgtggt ttctccagat
     3661 agtagacatc ctgtttcagg cgcgtctctt gtaaaccttc gccggaggag ggggaggtcg
     3721 tcgcggcaat gttgtcgtag atctgccagg tcacgtaacg ccaggtatcg acgcgctttt
     3781 ggattgcgtg ggtaatgtcg acaatctggt aacttttatc tttcagccat gcgttaacgg
     3841 cgctttgtac cattaccccc atcgtcacca gtaacacaat gatcaacagt aaaaagaagc
     3901 gggtaatgct ccccggtagg agtgaaaagc gggtcgtggc cgttgtctct ttctgacgca
     3961 ttcgtgttta tgacctgtta aaacttcgcg aagtgatagg gcagtataaa gggtacagtg
     4021 tgaaattcca gactcttacc tgattgatga ttctctattc tcccgtgata tttatcatcc
     4081 tgtatggggg aaatagatgt acaagcgcca ttttgctgta caaataacct acaaaaagcc
     4141 caacgaaatt aattgcaaat aaacgaagat tgctggaaat tatgctgatg ttaattattt
     4201 gtgaaatagt taacaagcgt tatagttttt ctgtggtagc acagaataat gaaaagtgtg
     4261 taaagaaggg taaaaaaaac cgaatgcgag gcatccggtt gaaatagggg taaacagaca
     4321 ttcagaaatg aatgacggta ataaataaag ttaatgatga tagcgggagt tattctagtt
     4381 gcg
//
LOCUS       DAZGXZ010000252         4319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000252 DAZGXZ010000000
VERSION     DAZGXZ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.253"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(37..618)
                     /locus_tag="ACHKJF_005246"
     CDS             complement(37..618)
                     /locus_tag="ACHKJF_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0952434.1"
                     /translation="MALLGYARVSTSHQKLTHQISELKNAGVREDRIFTDMMTGATDE
                     REGLQRLLARAEKDDVIICTKMDRLGRNTADMIRVVDSCYKKGIAIRFLENGLSTEGT
                     MGKMVIQILAAVAEAERERILERTNDGRIAALAAGVKFGRKKHPRTPTALELISQGES
                     LGSVTEKTGISRSTYFRLKRTIKNDAKIATFYK"
     gene            775..3813
                     /locus_tag="ACHKJF_005247"
     CDS             775..3813
                     /locus_tag="ACHKJF_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP0952435.1"
                     /translation="MQKYKKRISILTKSEINELYQVPSFNPVERDEFFSLDISLKREI
                     DKIINIESRVYLILITGYFRYKPVIPEFTLKDVKRDVKYIIQTYYKNESTNLNLNIPK
                     STKYRLVKRMLSTLGFEQLTPSIKNELSERLNDVATICMEPKYIFDELLAFLGQRRIA
                     LPGYSTVQNLVSDALNFERKRTINILSKRIYPATAQKITSIIHDDGILNSIRGYNYST
                     RDFSASEIENELRTHNVISSIYYEIKDIINSLGISQGNMEYYATIVKHSTTFRLKRHP
                     KWQGILYLCCYLYFRYREINDKIITAFRYLVKKHKDASVLFAKARATEEIDLINKKLK
                     FASKIFNFFVDSNIEDSIPFGYVREKVFSLISKDDMHLVSEFFDKNKIDVIEYQWQYI
                     DSHHLKIANSIRKLFLAIDIVYDNEKSVIGQQITAAKDDLRGKGKLDAKDLRVIHPSE
                     KNYIVKEGEINTQRFEFHMYSRVSNHLENGVFYAAESEQNKRLEDDLIERSYWHKNKS
                     ILIEKTGLERLITPITQTLNELENTFSDKLARITIGINSDANEFVKKQPHSNRLQWSL
                     ASKKWKTSIDNPIYNQIKNIGIVDVMKYVNNETNFLSVFNAISSRKNNLEADYNDLLA
                     CIFGNGANYGVHRMASASDRSIGILRDVNDKFIRPETTGMANTVISDAIANLSVYKYW
                     TINEDSPFGSVDGQKHTCRINTFKARYSAKYFRKGKGVSAMTLVSNHVPLSTVVISPN
                     EYEGHFAFDLLYNNTSEIQPKSLATDNHGINNVNFAILDIFGYQFSPRYAKFKKIFNE
                     LFDVILESDKLIIKLKKQFNFKLIEHEWENIQHIICSLSRKTTIQSTVVKKLSNTKRS
                     NKTLAALREYDRVIKCIYVLDYADNKTLRQFVQQALNRGEAYHQLRRAIASVNGNQFR
                     GGNDYQIDQWNDCARLIANCVIYYNSAILSRLVEKFEKENNKKAIDVLANLSPVAWRH
                     ILLGGNYSFEDQVAITNLDSLLEGVDPLSDIGDIESEYDG"
     gene            complement(4018..4215)
                     /locus_tag="ACHKJF_005248"
     CDS             complement(4018..4215)
                     /locus_tag="ACHKJF_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP0952436.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQTHFEAQYGIEQP"
BASE COUNT         1402 a          813 c          837 g         1267 t
ORIGIN      
        1 ctaaaagtct caccttgctt aggtaattaa ttgaatttat ttataaaacg ttgcaatttt
       61 agcatcattt tttattgttc tttttaacct gaagtacgtc gatcgagaga tacctgtttt
      121 ttctgtcaca ctgcccagag attctccctg tgatataagc tccaacgctg taggcgttcg
      181 tggatgcttc ttcctgccaa attttacgcc tgcggcaagt gccgctattc ttccgtcgtt
      241 tgtccgctcc agaatacgct cacgttccgc ttcagctaca gcagcaagga tttggataac
      301 cattttgccc attgtccctt ctgtactgag accgttctcc aggaaccgga tggcgatccc
      361 ctttttgtaa cagctatcaa ccacacgaat catgtcagca gtgttacgcc ccagtcggtc
      421 catttttgta cagatgatta cgtcgtcttt ctctgcacgg gccaacaacc gctgtagtcc
      481 ttcccgttca tcagttgcgc cagtcatcat atccgtaaaa atacgatctt cccgtacccc
      541 ggcatttttt agctccgaga tttgatgtgt tagcttctga tgactggttg atacccgtgc
      601 ataccccaat aacgccattt ttagtctcac ttatcgtttt tgacactgca tggtatcata
      661 atgtacaaaa tacgattttt gaaacctgct tttatgctgt tttagacagg tatcagatat
      721 cttaatattt gatactagcc atatttaaca aatatttagc attttggtaa atgtatgcag
      781 aagtacaaga aaagaattag tatattaaca aaaagtgaaa taaatgaatt atatcaagtc
      841 ccctctttta atcctgttga aagggatgag tttttttcgc tggatatcag ccttaaaaga
      901 gaaattgata aaataataaa tatagaaagc agggtgtatc tgatattaat aacagggtat
      961 ttcagatata aacctgtgat tcctgaattt acattaaaag acgtaaaaag ggatgtgaaa
     1021 tatattatcc agacatatta taaaaacgaa agtactaatc tcaatttaaa catacctaaa
     1081 agtacaaagt accgccttgt taaacgaatg ctttctactc taggatttga acaactcacc
     1141 ccatctataa aaaatgagtt aagtgaacgt ctgaatgatg ttgctactat atgtatggaa
     1201 ccaaaatata tttttgatga attacttgct tttttaggac agcggcgtat agctcttcct
     1261 ggatacagta ctgtccagaa cctcgtttca gatgctttaa actttgaaag gaaaaggaca
     1321 attaatattt tgtcaaagag aatttatcct gccactgcac aaaagattac atccatcatc
     1381 catgatgatg gaatattaaa ttccatcagg ggatataatt atagcacgag agacttctca
     1441 gcatcagaaa tagaaaatga attaagaact cacaatgtga taagtagtat ttactatgaa
     1501 ataaaagata taattaattc attaggtata tcccagggaa acatggagta ctatgcaact
     1561 atcgtaaaac acagtacaac attcagactt aagcgacacc ctaaatggca aggaatactt
     1621 tatttgtgct gctacttgta cttcagatac agagagataa atgataaaat aatcacagcc
     1681 ttcagatatt tagtaaaaaa acataaagac gcttcagttt tgtttgcaaa agctcgggca
     1741 acagaggaaa tagatctaat aaataaaaag cttaagtttg cgagtaaaat atttaacttc
     1801 ttcgttgata gtaacattga agacagcatc ccttttggat atgttcgtga aaaggttttt
     1861 tcacttatat cgaaagatga tatgcatctt gtgagtgaat tttttgacaa aaataaaatc
     1921 gatgtaattg aatatcaatg gcagtatatt gacagccatc accttaagat cgccaattca
     1981 atccgaaagc tttttctggc tatagatata gtatatgaca acgagaagtc tgttattggc
     2041 cagcaaatta ctgctgccaa ggatgatctc agaggaaaag gaaagctgga tgctaaggat
     2101 ctgagggtaa ttcatccatc cgagaaaaat tacattgtaa aagaaggaga aattaacact
     2161 caacgattcg aatttcatat gtacagcagg gtatctaacc atctggaaaa cggtgtcttt
     2221 tatgctgcgg agagtgaaca aaacaagaga ctggaagatg atctcataga acgaagttac
     2281 tggcataaga ataaatcgat tttgatagag aaaacaggcc ttgaacgact tatcacacca
     2341 ataacgcaga ctcttaatga gctggagaat acctttagcg ataaactggc tcgtataacc
     2401 attggaataa actcagatgc taatgagttt gtgaaaaaac aaccacactc caacagatta
     2461 cagtggagtc tggccagtaa aaaatggaag accagcatcg ataatccgat ttacaatcag
     2521 ataaaaaata tcggaatagt tgacgtaatg aagtatgtaa acaatgagac gaattttttg
     2581 tctgtgttta atgccatatc atccaggaaa aataatcttg aagctgacta taatgacctt
     2641 cttgcctgta tatttggtaa tggcgcaaat tatggtgttc acagaatggc atctgcatca
     2701 gaccgtagta ttggaatact acgtgatgtg aatgacaaat tcattcgtcc agaaacaaca
     2761 ggaatggcca acacagtaat atctgatgca attgcaaatc tttctgtata taaatactgg
     2821 acgataaatg aagattctcc atttggaagt gttgatgggc agaagcacac ttgcagaata
     2881 aatacattta aagcccgtta ttctgccaag tatttcagaa aggggaaagg ggtttctgcg
     2941 atgacactgg tatctaatca tgtgccatta tcaactgtgg taatttctcc aaatgaatat
     3001 gaaggtcatt ttgcatttga cttgttatat aacaacacca gtgaaataca accaaagtca
     3061 ttagcgactg acaatcatgg tataaacaac gttaattttg cgattcttga catttttggc
     3121 tatcagtttt caccacgtta tgcaaaattc aagaaaatat ttaatgaatt atttgatgtt
     3181 atactggaga gtgataaact cattataaaa cttaaaaaac aatttaactt taagctcatt
     3241 gaacatgaat gggaaaacat ccaacatatt atttgttctt taagcagaaa aacaaccatt
     3301 caaagtacgg ttgttaagaa gttatctaat acaaagcgta gcaacaagac attagcagca
     3361 cttcgggagt atgaccgagt tataaaatgc atatatgtcc tggattatgc tgacaataaa
     3421 accttaaggc aatttgtgca acaagcactg aacagaggtg aagcttatca tcaactaaga
     3481 agagcaatag catcagtaaa tggaaatcaa tttcgcggtg gtaatgacta ccaaatagat
     3541 caatggaacg actgtgcgag gttaatagct aattgtgtaa tctattataa ttcagcaata
     3601 ttatccaggc tggtcgagaa atttgaaaaa gaaaataata aaaaagcgat agatgttctg
     3661 gctaacttat ccccagtagc atggagacac attctccttg gagggaatta ttcttttgaa
     3721 gatcaagttg cgatcacaaa tttggacagt cttttggagg gagtagaccc actaagtgat
     3781 atcggagata ttgagtccga atatgacggt taacaacatg ataaataatg gtttttatgc
     3841 taagagtgag acttttggag gaaatgggcg cagaacccct atacactccg ctaccgctac
     3901 gtgactgggt catggctgcg ccccgacgcc cgctaaagcc ccctgacgtg ccctgacggg
     3961 ctttgtctgc tggcgaaaac actcacggac aagccgcgac cactttcgcc agcgccttta
     4021 tggttgttct atgccgtact gagcttcaaa gtgagtctgt atgctgttct ggagttcttc
     4081 cgccagttcg tgcagtcgtt cgcacatggc ggcctgttcg tctgcatcca gtgcatccag
     4141 tttttcgagc aacgtcaggc tctggctttt gataaatcgc gccatgttga gcgcggtctg
     4201 ctgctgtttg ttcacgttct cccgtctcct ttactggtgt ttacgtccgg cagtgcgtca
     4261 cctggtgtga tgacccggtg acgtcactga aaattatcac acaagcccag tcgcgcgtg
//
LOCUS       DAZGXZ010000253         4254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000253 DAZGXZ010000000
VERSION     DAZGXZ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.254"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHKJF_005249"
     CDS             complement(<1..209)
                     /locus_tag="ACHKJF_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0952437.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKJF_005250"
     tRNA            complement(324..399)
                     /locus_tag="ACHKJF_005250"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKJF_005251"
     tRNA            complement(406..480)
                     /locus_tag="ACHKJF_005251"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKJF_005252"
     tRNA            complement(597..681)
                     /locus_tag="ACHKJF_005252"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKJF_005253"
     tRNA            complement(690..765)
                     /locus_tag="ACHKJF_005253"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKJF_005254"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKJF_005254"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP0952438.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKJF_005255"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKJF_005255"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP0952439.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKJF_005256"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKJF_005256"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP0952440.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1094 a         1057 c          953 g         1150 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttatctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtc
//
LOCUS       DAZGXZ010000254         4169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000254 DAZGXZ010000000
VERSION     DAZGXZ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.255"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(12..1988)
                     /gene="speA"
                     /locus_tag="ACHKJF_005257"
     CDS             complement(12..1988)
                     /gene="speA"
                     /locus_tag="ACHKJF_005257"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP0952441.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INTAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(1997..2128)
                     /gene="yqgB"
                     /locus_tag="ACHKJF_005258"
     CDS             complement(1997..2128)
                     /gene="yqgB"
                     /locus_tag="ACHKJF_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP0952442.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2222..2365)
                     /gene="yqgG"
                     /locus_tag="ACHKJF_005259"
     CDS             complement(2222..2365)
                     /gene="yqgG"
                     /locus_tag="ACHKJF_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP0952443.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2476..2727)
                     /gene="yqgD"
                     /locus_tag="ACHKJF_005260"
     CDS             complement(2476..2727)
                     /gene="yqgD"
                     /locus_tag="ACHKJF_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP0952444.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2783..3937
                     /gene="metK"
                     /locus_tag="ACHKJF_005261"
     CDS             2783..3937
                     /gene="metK"
                     /locus_tag="ACHKJF_005261"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP0952445.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLREAAGLK"
BASE COUNT         1056 a         1124 c         1006 g          983 t
ORIGIN      
        1 aataatctta attattcatc ttcaagataa gtataaccgt acaaacctgc ctcgaactct
       61 tcaaggaact gttgttgcag ttcagcatca agatcggttt tcttcacttg atcgcggaac
      121 tgggttaaca gcgttttcgg atcgagctgt acatattgca gcatgtccgc cacggtatcg
      181 ccttcgtcag acagttctac ttctacgcta ccgtcaggga acacgaacac gtcaaccgct
      241 tcggtatcac cgaacaggtt gtgcatattg ccaaggatct cctgatatgc gccgaccata
      301 aagaaaccga gcatcggcgg attctctgga tcgtactccg gcattggcat tgtcgtggca
      361 ataccatcac catcaatata gtggtcgata gcaccgtcag agtcacaggt aatatccagc
      421 agcacagcgc ggcgttccgg cacttgatcc agcccttcca gcggcagaac cgggaacaac
      481 tggtcgatcc cccacgcatc cggcatcgac tggaacagcg agaagttgac gtacattttg
      541 tctgccatac gttcctgcag ctcgtcgata atcggacgat gagcacggtt ttgcggatcc
      601 agttgcttct gcacttcatg gcacatgctc agatagagtt gttctgccca tgcacgttct
      661 tgcaggctaa aggtgccgga agagtagccg atatgaatgt cgtgcagatc catctggctg
      721 tcgtgtaacc attcacgcaa agaacggcga gttcccggtt cgtgcatctc ctgccaggtt
      781 tcccacatgc tttgcagcgc gcgcggcgca tcttctgcgg gcgcggtcgg caccgtatat
      841 tcgttacgtt ccacgccgat gatattcgat accagcacgg tgtgatgcgc agtcactgca
      901 cgacccgatt cggtgattac cgtcggatgc ggcagaccgt tttcttcaca cgcatcacca
      961 atcgcccaga tgatgttgtt ggcatattca ttgaggccgt agttcaccga acagtcggac
     1021 tgcgaacgag taccttcata atccacgccc agaccgccgc cgacgtcgaa gcactgaata
     1081 ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg attcacgaac gcctgtcgcg
     1141 atatcgcgaa tattcgccat ctgcgaaccg aggtgaaagt gcagtagttg caggctgtcg
     1201 agacgcccgg cttcacgcag ggtttcaacc agttgcagta cctgagtcgc agccaggccg
     1261 aacttcgatt tttccccgcc ggaggactgc catttacccg agccctgcga agccagacgt
     1321 gcacgcacgc ccagacgagg aacgacattc agacgttctg cttcatccag cacaatggcg
     1381 atttctgaca tcttctcaat gaccagatag accttgtgcc ccatcttctc gccaattaat
     1441 gccaggcgga tatattcgcg gtctttataa ccgttgcaga cgatgacgct acgggtcatg
     1501 ccagcatgtg ccagcactgc catcaactcg gctttggaac cggcttccag acccagcggt
     1561 tcgcccgaat gaatcaggga ctcgatcacg cggcggtgct ggttaacttt gatcggataa
     1621 acaaggaagt aatcgccgtt atagccgtag gattccctcg cacgtttgaa cgcggtgtta
     1681 atggaacgca aacggtgctg caggatctgt gggaaacaga acagtgcagg cagacgctga
     1741 ccctgtgctt cacgagtttt cactaactgc gcgagatcga cgcgagcttc cgggacgtcc
     1801 gggtccgggc acacgctaat gtggcccagc tcgttaacgt catagtagtt attgccccac
     1861 caggcaatat tgtaagtacg cagcatcttg ctggcttcct gggagctcat tgcaacctcc
     1921 tgcatggagc gtagtacacc gtgttcgccc gctgacgaag gcaaacccat agacatgtcg
     1981 tcagacatag cgaacctcaa attattttat taagtgtaaa acagttaacg actatcgcag
     2041 cctggaactg cgataacaac ccataagcac atggattttc cagcagtgta tgctgacgct
     2101 ccgactgcgc aaccggtttc tttttcataa cattattaag cacataaccg aacgtaagtg
     2161 tgacagttcg gcgaaaccac gagaaaactc ttgtttttac aagagcgccc ttgttcagtc
     2221 ctcagtaact gtaaccagct cttgaatcct gagaagcgcc gagatgggta taacatcggc
     2281 aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa cggtgagaca
     2341 ggttaagcag cagacaacgg ttcattattt cgtatcacct ccacggccgc ctgttaagac
     2401 gaacccacaa gccaaaactc tgatttcaac ccggctggaa gtggcaacac gaaagaaacg
     2461 tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac cccacaatgg cagaaaattg
     2521 caaaagataa atacgcagaa tgccggcatt gtcaggaaaa atttccagcc acgtttttaa
     2581 cagaatgaga cacgattcaa aaaaaagtgg aaatcgggtg aagaattgac ctaaaatagc
     2641 catccagatg ttaatccatc cataccgatt aacactcaga ctgccagtgt ttttaacctg
     2701 cagagtcgtg gtaggatccg ctaccacaga aaatccacac aacagtttga gctaaccaaa
     2761 ttctctttag gtgatattaa atatggcaaa acaccttttt acgtccgagt ccgtctctga
     2821 agggcatcct gacaaaattg ctgaccaaat ttctgatgcc gttttagacg cgatcctcga
     2881 acaggatccg aaagcacgcg ttgcttgcga aacctacgta aaaaccggca tggttttagt
     2941 tggcggcgaa atcaccacta gcgcctgggt agacatcgaa gagatcaccc gtaacaccgt
     3001 tcgcgaaatt ggctatgtac attccgacat gggctttgac gctaactcct gtgcggttct
     3061 gagcgctatc ggcaaacagt ctcctgacat caaccagggc gttgaccgtg ccgatccgct
     3121 ggaacagggc gcgggtgacc agggtctgat gtttggctac gcaactaatg aaaccgacgt
     3181 gctgatgcca gcacctatca cctatgcaca ccgtctggta cagcgtcagg ctgaagtgcg
     3241 taaaaacggt actctgccgt ggctgcgccc ggacgcgaaa agccaggtga ctttccagta
     3301 tgacgacggc aaaatcgttg gtattgatgc tgtcgtgctt tccactcagc actctgaaga
     3361 gatcgaccag aaatcgctgc aagaagcagt aatggaagag atcatcaagc caattctgcc
     3421 cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc gtttcgttat
     3481 cggtggccca atgggtgact gcggtctgac tggtcgtaaa attatcgttg atacctacgg
     3541 cggcatggcg cgtcacggtg gcggtgcatt ctctggtaaa gatccatcaa aagtggaccg
     3601 ttccgcagcg tacgcagcac gttatgtcgc gaaaaacatc gttgctgctg gcctggccga
     3661 tcgttgtgaa attcaggttt cctacgcaat cggcgtggct gaaccgacct ccatcatggt
     3721 agaaaccttc ggtactgaga aagtgccttc tgaacagctg accctgctgg tacgtgagtt
     3781 cttcgacctg cgcccatacg gtctgattca gatgctggat ctgctgcacc cgatctacaa
     3841 agaaaccgca gcatacggtc actttggtcg tgaacatttc ccgtgggaaa aaaccgacaa
     3901 agcgcagctg ctgcgtgaag ctgccggtct gaagtaatct ttcttcacct gctttcaaag
     3961 gccagcctcg cgctggcctt tttcttttac ctcaccgcgg tggataggcg ttcacgccgc
     4021 atccggcaaa aagctgcccg cacaataaca tcattcttcc tgaacacgtt ttatcgcaaa
     4081 ttatcatcac tactgaaacc gattacacca acaacaccag acaaatattt gtaatatttt
     4141 catattatta ttcagttttc acagttgtt
//
LOCUS       DAZGXZ010000255         4087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000255 DAZGXZ010000000
VERSION     DAZGXZ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.256"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..3067)
                     /locus_tag="ACHKJF_005262"
     CDS             complement(<1..3067)
                     /locus_tag="ACHKJF_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ag43/Cah family autotransporter adhesin"
                     /protein_id="HFP0952446.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLINHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WINAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSSAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIHEGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     IQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGIY
                     GAAGHSSVDVKDDDASRAGTVRDDAGSLGGYLNLTHTSSGLWADIVALGTRHSMKAST
                     DNNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNAGYVKFGYG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSTKHGVSELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITLGVQAGYAHSV"
     gene            complement(3401..>4087)
                     /locus_tag="ACHKJF_005263"
     CDS             complement(3401..>4087)
                     /locus_tag="ACHKJF_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0952447.1"
                     /translation="PVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDAEYEA
                     LYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPCHEWD
                     MAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVTVSARTGWELDTLVSALMTALPDHAA
                     SPLMTRLQDGLRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSV
                     ARAVWNWIFF"
BASE COUNT          808 a         1230 c         1118 g          931 t
ORIGIN      
        1 tgacgctgtg ggcataaccg gcctgaacgc ccagggtgat attttcccgg acacgggctt
       61 ccagtccggc ctgcaggtcc agtgacgtgc cattccggga cggtgagaac gtcatgttac
      121 tgccggctgc agctgtaccc atgctcatgt ccccccggga gctgaaggtg cggataacag
      181 aaggttgtac ccaccagttc acaggcagtt cactcacacc gtgttttgta ctgtcgcgca
      241 gggtgtcacg ggatgaggtg ccttcaccaa aggtcatatc gttgtggctg cccagacgga
      301 aaccggcacg cacatgttgt gcactgccat acccgaactt cacataaccg gcgttatcct
      361 ggccgtcatc cagggagagt ccctgccagg tgtactgcag ttgcggctcc agcatcagat
      421 tgtcagtgat actgaagggc agaccggttt ccagtgaacc cagccagccc cacccccggg
      481 cgcggaagtc gttattgtcc gttgacgctt tcatgctgtg gcgggttccc agggccacaa
      541 tgtcagccca caggccggag gatgtatgtg tcagattcag gtatccgcca aggctgccgg
      601 catcatcccg gaccgtgccg gcacgggagg cgtcatcatc cttaacatca acggaagaat
      661 ggcctgcagc accatatatc cctgcggtca cagacatacc ggcaacctct gttctcagca
      721 ggtcaccctc caggcggacg aagccatagc tgccgctgct ttccggcgtg gccccacggg
      781 caataccgcc gttgttatcg tgaccgagat ggccgccctg aatgctgaga cggacgctgt
      841 tattttcacc gtttacaccg gtctgatggc tgcgggagcc tgccagaatc cggtcatagt
      901 ccattgcctg tgtcagcatg gaggcgtaca gggggacttc agcacgataa cgttcttcac
      961 tgcgcagata ccagctctca tcactgtccc ggttgagggt gtagttaaag gccccggcct
     1021 gcagcatatt cccctggaca aaagcccctt cctccgtggt ggcaccgtta atggcctcaa
     1081 ccacctgaat acccttaccg gtggtcgcca gccccgtacc actgttgccg gcgttcacca
     1141 gattcaggat ggtttttccg gttgccctgc caccgtcaat gaccagtctg tcagcattgt
     1201 tctgcgccat atccgggcgt acacgcaggc tgatggtgcc attctgcccg ttcaggtttt
     1261 tcacctgcag ggttgtcgga acgaacttcc ctgtgcgggc ggaggtgaaa tgaatctgtc
     1321 cggcatggct gaggtcatcc accaccgact gaaccgtggc gttatcgggg atattccagg
     1381 tggcaccgga tgtcagggtg acattcgtgg ggtcaatggc accgttcagc actgtgctgc
     1441 cggtcagctt cagggccgtg ccgcgctgag cgatgacatc cgtggtgacg gtactgtcgt
     1501 tcagcgtcag cgtgccttca ttcaggttga cggttttctg gatgagtgtg gtgttgctga
     1561 cagtgagtgt gccacttcct gatttttcca ccctgccgtt accggtaaga gcgcctccct
     1621 gcaggagtgc atcaccttca tggatggtca gggtatcgtt attcaccgtt ttcccggaaa
     1681 gggtaagcgt ggcaccgtta ttcagcgtgg tggtgcccgc cagcgagcca cctctggcgg
     1741 tgaacagtcc gccatttacc gtggtatccg tggccgtatc accggcgttc agtgtgaagg
     1801 aactgccttt ttccagcatc agggcatccg cctgaccgcc tccgatacgg aatgccgttc
     1861 cgtcgctccg ggtaccactg acagcggcac cggaatcggc cagcagtaca ccgccattcc
     1921 ccatggatac ggtggtggcg gcaccaccgt tgcggacatc cagcgttccg ccatcatcca
     1981 cacgggtatt agtggctgtg tgtccgctca gcacatccag acgtccgcca ttttccagta
     2041 cgacattatc agccttaccc gccacaacgg agaatgctcc caggcggttt gtaccggtga
     2101 cggttgcagc tgaactggta accagtgcac cgcccgtgtt ctgggtgaca tcagacgctt
     2161 taccgccggc attcacctgc agctttcctt tctggttgat ggtggtatgc gcggcagttc
     2221 ctccttcctt aatcacctgc cagccatcac ggtttatcag cgtctctgtt gccgtgccac
     2281 cgttgtgtac atactggtta cccccctcca gccgggtgtt atgtgcctct ccgtgtaccg
     2341 tctggtcacc accggcgtaa atgagggtgt cccgtgccat ccccgaagac cagataacct
     2401 gccggccatt tttgttgatg gtggtggatt cagccgtccc tccgaccatc tgaccgctgg
     2461 acacattttc agactccgga ccaccttctg caccggtgtt gacgatggtt ccggttgcca
     2521 gtccgccatg ttttatggtc tggtaaccat cctggttaat aattgtaccg gcagctttcc
     2581 ctcccccgtg tacccactgc tcacctctgt tatccagcgt ggtgttcacc gccagtccgt
     2641 taaggctctg ccctccgcca tcacgaataa ccgtatcact ggcagttcct cctgcctgca
     2701 caatctgacg accatttgcg gtgacagtgg tgtttctgcc tgtgccaccc gcgtttatcc
     2761 attgcccgcc ggtgttttcg tcactgtccg gccccagctc aagcccggta ctgacagtca
     2821 cgccatcagc tgttccggat acaaactggt tgtcatggtt tatcagtgtt ccgccattca
     2881 ctgtttcacc cgggtgcaca acgatgtcag cagccagcac cgggagtgac gtgaccgcgg
     2941 caagagacag tgcaaccgcc acaccgccac gtttaccccg tgcgcgggcc agttcggagg
     3001 caaccacgaa agcgcccgta atgtgattcc ataccagcct gtagcaggta ttcagatgtc
     3061 gtttcatcag catttcctta atcagagtga gggtgaataa aaaagccggt atccacagat
     3121 gtggataccg gcaggtaaag ggttacagtg ccagaggtcc ggctgacgga agtttctgaa
     3181 ttgtggttct gttgttccct gaatctcatg tctgtgtgta tctgcgcacg ttgctaacag
     3241 gagttattat tagcataacg atcgatatta acgatcggtg aaataggtat cgatcgtttt
     3301 attctattgt caagctattg cacggacaag agagacagta acgaaaacag cgcttttgtg
     3361 agcagaccca gaggcaggga ggacatcatc cacgacaggt tcagaagaaa atccagttcc
     3421 ataccgcacg ggcaacagag accaccgtgt cacgaacagc gcgcagggcc gtacggacaa
     3481 cagaggcaac acagacgctc tccgctgtgt caaatatccg gtccaccgca ccggtaaact
     3541 gttcacgggc ctgagagcgg acagattccg tgcgcagccc gtcctgcagt cgggtcatca
     3601 ggggactggc ggcatggtcg ggaagcgctg tcatgagcgc actgaccagc gtatccagtt
     3661 cccagccggt gcgggccgat acggtcacaa ccggatgtac gggccggaac agacggaata
     3721 ccgcctccgt tttctcccga atgttctgtg cctgtgcggg agaaggctga ataccggcca
     3781 tatcccattc atggcagggc tccgttttat cggcctgcgt caccacaaac agtacccgct
     3841 gatgtccccg gtgcagtatg tgtcgccaga aatactcatc cacagacagg gcacggtcat
     3901 cggctttaat cagccacagt accaggtcca gttctggcag aatgtcacgg tacagggcct
     3961 catactctgc atccctgtcc cggctctcgc ccaccccggg caggtcagtg ataaccatgc
     4021 tgtgaccgtg accactcaga cggaagcgct gcacttcccg ggtgccggcg tgaacatcac
     4081 tgaccgg
//
LOCUS       DAZGXZ010000256         4079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000256 DAZGXZ010000000
VERSION     DAZGXZ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.257"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..687
                     /locus_tag="ACHKJF_005264"
     CDS             <1..687
                     /locus_tag="ACHKJF_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0952448.1"
                     /translation="PVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDAEYEA
                     LYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPCHEWD
                     MAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAA
                     SPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDTVVSV
                     ARAVWNWIFF"
     gene            1015..>4079
                     /gene="agn43"
                     /locus_tag="ACHKJF_005265"
                     /gene_synonym="flu"
     CDS             1015..>4079
                     /gene="agn43"
                     /locus_tag="ACHKJF_005265"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFP0952449.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARARGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNIP
                     DNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNNA
                     DRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFVQGNRLQ
                     AGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGE
                     NNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLMRTEVAGMSVTAGVYG
                     AAGHSSVDVKDDDGSRVGTVRDDAGSLGGYLNLIHNASGLWADIVALGTRHSMKASTD
                     NNDFRARGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGKDNAGYVKFGHGS
                     AQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSELPVNWWVQPSVIRTFSSRGD
                     MRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSV"
BASE COUNT          936 a         1110 c         1223 g          810 t
ORIGIN      
        1 ccggtcagtg atgttcacgc cggcacccgg gaagtacggc gcttccgtct gagtggccac
       61 ggtcacagca tggttatcac tgacctgccc ggggtgggcg agagccggga cagggatgca
      121 gagtatgagg ccctgtaccg tgacattctg cctgaacttg acctggtact gtggctgatt
      181 aaagccgatg accgtgccct gtctgtggat gagtatttct ggcgacacat cctgcaccgg
      241 ggacatcagc gggtactgtt tgtggtgacg caggccgaca aaacggagcc ctgccatgaa
      301 tgggatatgg ccggtattca gccttctcct gcacaggcac agaatattcg cgaaaaaacg
      361 gaggcggtat tccgtctgtt ccggcccgta catccggttg tggccgtatc ggcccgcacc
      421 ggctgggaac tggatacgct ggtcagtgcg ctcatgacag cgcttcccga ccatgccgcc
      481 agtcccctga tgacccgact gcaggacgag ctgcgcacgg aatctgtccg ctctcaggcc
      541 cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca cagcggagag cgtctgtgtt
      601 gcctctgttg tccgtacggc cctgcgcgct gttcgtgaca cggtggtctc tgttgcccgt
      661 gcggtatgga actggatttt cttctgaacc tgtcgtgact gatgccctcc ctgactctga
      721 gtctgctcac aaaagcactg ttttcgttac tgtctctctt gtccgtgcaa tagctcaata
      781 atagaataaa acgatcgata tctattttat cgatcgttta tatcgatcga taagctaata
      841 ataacctttg tcagtaacat gcacagatac gtacagaaag acattcaggg aacaacagaa
      901 ccacaattca gaaactccca cagccggacc tccggcactg taacccttta cctgccggta
      961 tccacgtttg tgggtaccgg cttttttatt caccctcaat ctaaggaaaa gctgatgaaa
     1021 cgacatctga atacctgcta caggctggta tggaatcaca ttacgggcgc tttcgtggtt
     1081 gcctccgaac tggcccgcgc acggggtaaa cgtggcggtg tggcggttgc actgtctctt
     1141 gccgcagtca cgtcactccc ggtgctggct gctgacatcg ttgtgcaccc gggagaaacc
     1201 gtgaacggcg gaacactggc aaatcatgac aaccagattg tcttcggtac gaccaacgga
     1261 atgaccatca gtaccgggct ggagtatggg ccggataacg aggccaatac cggcgggcaa
     1321 tgggtacagg atggcggaac agccaacaaa acgactgtca ccagtggtgg tcttcagaga
     1381 gtgaaccccg gtggaagtgt ctcagacacg gttatcagtg ccggaggcgg acagagcctt
     1441 cagggacggg cagtgaacac cacgctgaat ggtggcgaac agtggatgca tgagggggcg
     1501 atagccacag gaaccgtcat taatgataag ggctggcagg tcgtcaagcc cggtacagtg
     1561 gcaacggata ccgttgttaa taccggggcg gaagggggac cggatgcaga aaacggtgat
     1621 accgggcagt ttgttcgcgg ggatgccgta cgcacaacca tcaataaaaa cggtcgccag
     1681 attgtgagag ctgaaggaac ggcaaatacc actgtggttt atgccggcgg cgaccagact
     1741 gtacatggtc acgcactgga taccacgctg aatgggggat accagtatgt gcacaacggc
     1801 ggtacagcgt ctgacactgt tgtgaacagt gacggctggc agattgtcaa aaacgggggt
     1861 gtggccggga ataccaccgt taatcagaag ggcagactgc aggtggacgc cggtggtaca
     1921 gccacgaatg tcaccctgaa gcagggcggc gcactggtta ccagtacggc tgcaaccgtt
     1981 accggcataa accgcctggg agcattctct gttgtggagg gtaaagctga taatgtcgta
     2041 ctggaaaatg gcggacgcct ggatgtgctg accggacaca cagccactaa tacccgcgtg
     2101 gatgatggcg gaacgctgga tgtccgcaac ggtggcaccg ccaccaccgt atccatggga
     2161 aatggcggtg tactgctggc cgattccggt gccgctgtca gtggtacccg gagcgacgga
     2221 aaggcattca gtatcggagg cggtcaggcg gatgccctga tgctggaaaa aggcagttca
     2281 ttcacgctga acgccggtga tacggccacg gataccacgg taaatggcgg actgttcacc
     2341 gccaggggcg gcacactggc gggcaccacc acgctgaata acggcgccat acttaccctt
     2401 tccgggaaga cggtgaacaa cgataccctg accatccgtg aaggcgatgc actcctgcag
     2461 ggaggctctc tcaccggtaa cggcagcgtg gaaaaatcag gaagtggcac actcactgtc
     2521 agcaacacca cactcaccca gaaagccgtc aacctgaatg aaggcacgct gacgctgaac
     2581 gacagtaccg tcaccacgga tgtcattgct cagcgcggta cagccctgaa gctgaccggc
     2641 agcactgtgc tgaacggtgc cattgacccc acgaatgtca ctctcgcctc cggtgccacc
     2701 tggaatatcc ccgataacgc cacggtgcag tcggtggtgg atgacctcag ccatgccgga
     2761 cagattcatt tcacctccac ccgcacaggg aagttcgtac cggcaaccct gaaagtgaaa
     2821 aacctgaacg gacagaatgg caccatcagc ctgcgtgtac gcccggatat ggcacagaac
     2881 aatgctgaca gactggtcat tgacggcggc agggcaaccg gaaaaaccat cctgaacctg
     2941 gtgaacgccg gcaacagtgc gtcggggctg gcgaccagcg gtaagggtat tcaggtggtt
     3001 gaagccatta acggtgccac cacggaggaa ggggcctttg tccaggggaa caggctgcag
     3061 gccggtgcct ttaactactc cctcaaccgg gacagtgatg agagctggta tctgcgcagt
     3121 gaaaatgctt atcgtgcaga agtccccctg tatgcctcca tgctgacaca ggcaatggac
     3181 tatgaccgga ttgtggcagg ctcccgcagc catcagaccg gtgtaaatgg tgaaaacaac
     3241 agcgtccgtc tcagcattca gggcggtcat ctcggtcacg ataacaatgg cggtattgcc
     3301 cgtggggcca cgccggaaag cagcggcagc tatggattcg tccgtctgga gggtgacctg
     3361 atgagaacag aggttgccgg tatgtctgtg accgcggggg tatatggtgc tgctggccat
     3421 tcttccgttg atgttaagga tgatgacggc tcccgtgtcg gcacggtccg ggatgatgcc
     3481 ggcagcctgg gcggatacct gaatctgata cacaacgcct ccggactgtg ggctgacatt
     3541 gtggccctgg gaacccgcca cagcatgaaa gcgtcaacgg acaataacga cttccgcgcc
     3601 cggggttggg gctggctggg ttcactggaa accggtctgc ccttcagtat cactgacaac
     3661 ctgatgctgg agccacaact gcagtatacc tggcagggac tttccctgga tgacggtaag
     3721 gacaacgccg gttatgtgaa gttcgggcat ggcagtgcac aacatgtgcg tgccggtttc
     3781 cgtctgggca gccacaacga tatgaccttt ggcgaaggca cctcatcccg tgcccccctg
     3841 cgtgacagtg caaaacacag tgtgagtgaa ttaccggtga actggtgggt acagccttct
     3901 gttatccgca ccttcagctc ccggggagat atgcgtgtgg ggacttccac tgcaggcagc
     3961 gggatgacgt tctctccctc acagaatggc acatcactgg acctgcaggc cggactggaa
     4021 gcccgtgtcc gggaaaatat caccctgggc gttcaggccg gttatgccca cagcgtcag
//
LOCUS       DAZGXZ010000257         4046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000257 DAZGXZ010000000
VERSION     DAZGXZ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.258"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..154)
                     /gene="ariR"
                     /locus_tag="ACHKJF_005266"
                     /gene_synonym="ymgB"
     CDS             complement(<1..154)
                     /gene="ariR"
                     /locus_tag="ACHKJF_005266"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP0952450.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNK"
     gene            complement(183..455)
                     /gene="ymgA"
                     /locus_tag="ACHKJF_005267"
     CDS             complement(183..455)
                     /gene="ymgA"
                     /locus_tag="ACHKJF_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP0952451.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(498..734)
                     /gene="ycgZ"
                     /locus_tag="ACHKJF_005268"
     CDS             complement(498..734)
                     /gene="ycgZ"
                     /locus_tag="ACHKJF_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP0952452.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1048..2259
                     /gene="bluF"
                     /locus_tag="ACHKJF_005269"
     CDS             1048..2259
                     /gene="bluF"
                     /locus_tag="ACHKJF_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP0952453.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            2464..3195
                     /gene="bluR"
                     /locus_tag="ACHKJF_005270"
     CDS             2464..3195
                     /gene="bluR"
                     /locus_tag="ACHKJF_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP0952454.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3416..3820
                     /gene="ycgX"
                     /locus_tag="ACHKJF_005271"
     CDS             3416..3820
                     /gene="ycgX"
                     /locus_tag="ACHKJF_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP0952455.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3873..3983
                     /locus_tag="ACHKJF_005272"
     CDS             3873..3983
                     /locus_tag="ACHKJF_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952456.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
BASE COUNT         1203 a          837 c          806 g         1200 t
ORIGIN      
        1 ttttattatt aacattatcg cctgaaagca ttaaattggt gacagcctgc cctaacactg
       61 ctgactcttc ttctaacaaa ttacccgaac tgcgaaagta acttgctaac gctttatcag
      121 ttattgcatt atgaattgta gtatcttcaa gcatggtaac ctctcatctt acttatgaaa
      181 ttttaatgta ttctgtttat tttcttacca ttgaacatac ttttattgtc agctgtcgct
      241 gttcttcggt ttggccctcc tgaatcacta ttgctaaaat cacgaattat gctgtcaatt
      301 aaatcgcgtc ggaaagcatc ttcctcagcc gatttcatta cgagtaattg cttaataaga
      361 gtctgcaatg aaaaatttat cttcatgcgc agtatctgga tcaccgcctg gccagtaatt
      421 tcatatttta tacgttcatt atcagatgtc ttcataacga cttcatagct aacctgttaa
      481 ataatcaggc tgttgcatta ttcaaaaagc aacccaatta gtgcattata atgtttctgt
      541 tcctcttccc cagtcgcgtg ttccagtcgg caaagaagtt tggcacaaag cgactttcga
      601 ctcagattac gtccatcttt caaaatctca gtcacaattt ccccgagagt ttcctgctga
      661 gtaggcaagt tagcttttgc aaagtaacga gtgattgctc ccgcagaatc taaagtcact
      721 gaattttgat gcatgctacg cctctgttaa aaatgttaat caatgttaag tgaggtaaca
      781 atagcaaact tgtacgaaat atgtgtacag aatgataaat taattttttg caattagtgc
      841 taatgcatat aaaacaatgt gttatgataa aacatattgc atcatttaac tgacaatcta
      901 tacaagattt ttcgccaact agtgttcaac tttttccatt ttttatcaat gtaaagaaac
      961 agttatgttt tggtattaaa agaaagacac aagaagatat gtctgttacc gtcttactct
     1021 cgcctcaccc attaccctgg attgttaatg cttaccaccc ttatttatcg tagccatata
     1081 cgtgacgacg aacctgtcaa aaaaatcgaa gaaatggttt cgatagcaaa tcgcaggaac
     1141 atgcagtctg acgtaacagg gatcttactg tttaatggtt ctcatttttt ccagcttctg
     1201 gaaggtccgg aagaacaggt taaaatgata tatcgggcta tatgccagga tccacggcac
     1261 tataatattg ttgagctgct gtgcgattac gcgcctgctc gccgttttgg caaagcggga
     1321 atggaattat ttgatttgcg cctgcacgag cgagatgacg ttttacaggc cgtattcgac
     1381 aaaggcacat caaaatttca gctaacttat gatgacagag cgctacaatt ttttcgtact
     1441 tttgtccttg caaccgaaca atcaacctat ttcgagatcc ctgccgaaga ctcctggctt
     1501 tttatcgctg acggatctga taaagaactt gattcctgtg ccctttcacc aactataaac
     1561 gaccactttg cctttcatcc tattgtcgat cccttatcgc ggcggataat cgcttttgaa
     1621 gccattgtgc aaaaaaatga agatagccca tcagccatag cggttgggca gcgtaaagac
     1681 ggggaaatct acacagcgga tctcaaaagt aaggcgcttg cattcacgat ggcacacgca
     1741 cttgagctcg gtgataaaat gatttcaatc aatctattac ctatgaccct ggttaacgaa
     1801 cctgacgcag tctctttttt acttaatgaa ataaaggcca atgctctggt gcctgaacaa
     1861 atcatcgttg aatttactga aagtgaagtc atatctcggt ttgatgagtt tgccgaagcg
     1921 attaaatcgc taaaggctgc tggtatcagt gtagcaattg atcattttgg cgcaggtttt
     1981 gctggtttgt tactcctgtc acgcttccag cctgacagaa ttaaaatcag tcaggaattg
     2041 attaccaatg ttcataaaag tgggccacgg caggcaatta ttcaggcgat cataaaatgc
     2101 tgtacatcac ttgaaattca agtcagtgct atgggcgtgg caacaccaga agagtggatg
     2161 tggcttgaat ccgcaggaat tgagatgttt cagggagatc tgtttgctaa agctaaattg
     2221 aatggtatcc cttccatagc gtggccagag aaaaaataat tttcagcaca ttctttcaca
     2281 tgatttcagt aaatcatcgt gaaaatgaat acgttacgtt taaatttaat ccacattaaa
     2341 aataatgttt catccaattt agctgaaatc ttatttatac tttgtacaat tttagcgtat
     2401 attgtacaaa gtattattgg gtcgtgtaca ggcgacggag atttgtgacc gcaaggagga
     2461 attgtggctt attacagcat tggtgatgtt gctgaacgtt gcgggattaa tcctgtcact
     2521 ctccgggcct ggcaacgccg ctacggtttg ttaaaaccac agcgcagtga aggcggacac
     2581 cgactctttg atgaagaaga catacaacgc atcgaagaga tcaagcgttg gataagtaat
     2641 ggcgtccctg taggcaaagt taaagcatta ctggaaacca ccagccagga tacggaagat
     2701 gactggagcc gcctgcaaga agagatgatg tcaattcttc gcatggctaa tcctgccaaa
     2761 ctacgcgcga gaattatttc actgggtcga gagtacccag tagatcaatt gattaatcat
     2821 gtttaccttc ctgttcgcca gcgtctcgtg cttgatcaca acacctcccg cattatgagc
     2881 agtatgtttg acggcgcatt aattgaatac gcagcaacct cgcttttcga aatgcgccgt
     2941 aagcccggta aagaagccat tctgatggcg tggaatgttg aagagagagc acgattgtgg
     3001 ctggaagcat ggcgtttatc attgtcagga tggcacattt ctgttcttgc tgatcccatt
     3061 gaatcgccgc gcccggaact gttcccgacg caaacattga ttgtctggac aggcatggca
     3121 ccaacgagaa ggcaaaacga acttttacaa cattggggtg agcaaggtta taaagtcatc
     3181 tttcatgccc cctaatttca cctttatccc gccccttaga cagaatgtta taatctgaac
     3241 ccacaacagc gtttgtaccg gagctggtcc gttgcaaaat gctcaccggg agaattcaga
     3301 tattccctct atcacactct gtacatatca cagccaaagg acagaccaga ttgaagtggt
     3361 aaaaccgatc gtgcacacta cgcagtctca caactaacaa gaggttatta tcctgatgga
     3421 tcaggtcgtt gtttttcaaa agatgtttga gcaagtgaga aaagagcaaa acttctcctg
     3481 gttttattca gaattaaaac atcaccgtat tgcacattac atttattatc tggctacgga
     3541 taacatcaga attattactc acgatgacac ggttttgtta ttaagaggaa ccaggaacct
     3601 gttaaaagtt agtacgacca agaaccctgc taaaataaaa gaggccgcat tgcttcatat
     3661 ttgcggaaaa tctacatttc gggaatactg ttcaacacta gcaggcgcgg gcgttttccg
     3721 gtgggttact gatgttaatc ataacaaacg cagttactac gccattgata atacgctttt
     3781 atatattgaa gatgtagaaa ataataaacc attaatctag ctaaagttgg atgcttaaga
     3841 aatgcttcat aattcagtaa ggcattagca taatgggaat aaaagtacag agactatccc
     3901 tatggatgat aaatactgtc cttttattgc tacccataaa taatcaccat gccaatacta
     3961 tcaaattgat atttgaaatg tgatcacttg actttctata cgttatttta taacggttaa
     4021 tatatttata aaaacaacgg gtgtgc
//
LOCUS       DAZGXZ010000258         3795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000258 DAZGXZ010000000
VERSION     DAZGXZ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.259"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..847
                     /locus_tag="ACHKJF_005273"
     CDS             <1..847
                     /locus_tag="ACHKJF_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFP0952457.1"
                     /translation="KPAGSLYLFCGHRLASDIEIMMRERFSVLNHIIWAKPSGRWNGC
                     NKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMAPLIAYFRDARATL
                     GITAKQIVDATGKKNMVSHWFSASQWQLPNEDDYRKLQVLFARVAEEKHQRGELEKPH
                     HQLVSTYSELNRQYASLLEEYKSLRRYFSVSAAVPYTDVWTHKPVQYYPGKHPCEKPA
                     DMLRQMITASSRPGDLVADFFMGSGSTVKAAMALGRRAIGVELEAERFEQTAMDVQNL
                     IRKRE"
     gene            888..963
                     /locus_tag="ACHKJF_005274"
     tRNA            888..963
                     /locus_tag="ACHKJF_005274"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:921..923,aa:Ile,seq:cat)
     gene            971..1047
                     /locus_tag="ACHKJF_005275"
     tRNA            971..1047
                     /locus_tag="ACHKJF_005275"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1005..1007,aa:Arg,seq:tcg)
     gene            1061..1137
                     /locus_tag="ACHKJF_005276"
     tRNA            1061..1137
                     /locus_tag="ACHKJF_005276"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1095..1097,aa:Arg,seq:tct)
     gene            complement(1260..1379)
                     /locus_tag="ACHKJF_005277"
                     /pseudo
     CDS             complement(1260..1379)
                     /locus_tag="ACHKJF_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1643..3592
                     /locus_tag="ACHKJF_005278"
     CDS             1643..3592
                     /locus_tag="ACHKJF_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0952458.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVPDTGAGGSPGVATTEPEYYYVIPLAGQSNGMAYGEGLPLPETY
                     DRPDSRIKQLARRSTVTPGGDTCAYNDVIPADHCLHDVQDMSALNHPHADLSKGQYGT
                     VGQGLHIAKKLLPYIPQNAGILLVPCCRGGSGLTVGNDGTFSETSGASANSARWGVGK
                     PLYQDFLFRTKAALLKNPKNRLLAVVWMQGENDLADGSQQHSGLFTAMVQQFRADMAA
                     YSAQCVGGSAGSVPWICGDTTYYWKNLNADKYEAVYGGYKGREAQNIFFVPFMTDSSG
                     ANTSTNNPSEDPDIPASGYYGAASRTSANWTSADRASHFSSWARRGIISDRLATAILV
                     HAGRAAEFITGKQPDTVKPTGPSGEGPEREAEAPVSNRTLMSLLASGEDLASQGWRYY
                     HKPASGDSIDKNIAEAVVSDEGATGGKALQLNKPESHIWFLEHDAAGQGAELLKKGGR
                     VSVRFKVPGSLVQNRFALGIYWQLSSLPEGVSLAEEGNDMLMSFFLQTDATNLNAMYH
                     KTQNAKLDTFGAFDNEWHTLSFEFAGNNSIQVTPVLDEKRGTPFTLVKSQATGVADKL
                     LLTDITKVATYTLLIDSVKVEVNNP"
     gene            3741..>3795
                     /locus_tag="ACHKJF_005279"
     CDS             3741..>3795
                     /locus_tag="ACHKJF_005279"
                     /inference="COORDINATES: protein motif:HMM:NF018789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0952459.1"
                     /translation="MTFIQLILLYFCTAVCVL"
BASE COUNT          921 a          900 c         1118 g          856 t
ORIGIN      
        1 gaaacctgcc ggaagtcttt acctgttctg tggccatcgc ctggcatctg atatcgaaat
       61 catgatgcgt gaacgcttca gtgtgctgaa ccatattatc tgggcgaagc cgtccggacg
      121 ctggaacggg tgcaacaagg aaagcctgag ggcgtatttc cccgccacag agcgcattct
      181 gttcgcggaa cattatcagg ggccgtatcg cccgaaagat gccgggtatg aggcgaaggg
      241 cagggcactg aaacagcatg tgatggcccc gctgattgct tactttcgtg atgcgcgcgc
      301 taccctgggg ataacggcaa aacagattgt ggatgccaca ggaaagaaaa acatggtgtc
      361 gcactggttc agtgccagcc agtggcagtt accgaacgag gatgattaca gaaaacttca
      421 ggtgctgttt gcccgggtgg cagaagagaa acatcagcgg ggtgaactgg aaaagccaca
      481 tcaccaactg gtcagcacat acagtgagct gaaccggcag tatgccagcc tgctggaaga
      541 gtacaaatca ctgcggcgtt atttttccgt atcggcagcc gttccttata cggatgtctg
      601 gacgcacaag cctgtgcagt attatccggg caaacatccc tgtgaaaaac cggcagatat
      661 gttgcggcaa atgattaccg ccagcagtcg tccgggtgac ctggttgcag atttcttcat
      721 ggggtccggt tcgacagtca aagcagcgat ggcgctgggg cgtcgtgcaa ttggtgtcga
      781 gctggaggct gaacgttttg agcagaccgc aatggatgta cagaatttaa tcagaaagag
      841 agagtgatat tgctgaattt attctgtaac gttatcatta tgttatcggc cctttagctc
      901 agtggtgaga gcgagcgact cataatcgcc aggtcgctgg ttcaaatcca gcaagggcca
      961 ccatcacata ccgccattag ctcatcagga cagagcgcta gccttcgaag ctggttgcgc
     1021 gggggtcggg tccccgatgg cggtccatta tcggtattca gcgttgttag ctcagccgga
     1081 cagagcaatt gccttctaag caatcggtca ctggttcgaa tccagtacaa cgcgccacac
     1141 ttattttccc tggctcgctt ttgcgggctt tttattacag gaaagacacc ggacagtgaa
     1201 atgttaaatg cctcacaatt caggcagttg atgattgtct ggttgacgga gagttgttaa
     1261 aaaagttttg catggtgaat ccccctgtgc ggaggggcaa tcagcgagta ggtatatggg
     1321 ataatcgcgg attcaggtgc tggtactgaa ttcaccggga ggcacccggc accatgcagt
     1381 tatgcaatgt aaatgttcac acaagcaaat cccctctccg gaggggattt ttttatgcaa
     1441 aaaaagcccg agtgggttcg ggcaacagca tgagatactt gcattgtcat ttttatcgtg
     1501 tggattttaa ccagggttta taaggctgcg caactgcgcg gcctttttcg ttttgcgggc
     1561 tgcggttctc ctcttttgat tctccgtgta gccggaccgt ggcccgcaac tgttgaggaa
     1621 aatcccggaa aggggaggaa taatggcatt taaacactat gacgtggtca gggcggcgtc
     1681 tccgtcagac cttgcggagc gactgacaca aaaactgaag gagggctggc agccgtttgg
     1741 cagtcctgtg gctatcactc cttatacact gatgcaggcc attgccgcag agggtgatgt
     1801 gaccacgcct gtggttgtgc ccgacacagg ggctggtggc tctccgggag tggctaccac
     1861 tgaaccggag tattactacg ttattcccct ggccgggcag tcgaacggta tggcctatgg
     1921 tgaggggctt cctctgccgg agacctatga tcgccctgac tcccgtatta agcagctggc
     1981 ccgtcgcagc actgtgacgc cgggtggtga tacctgtgca tacaatgacg ttattccggc
     2041 agaccactgt ctgcatgatg ttcaggacat gagcgcactt aaccatcctc atgcagacct
     2101 gagtaagggc cagtacggga ctgtcggtca ggggctgcat attgccaaaa aactgctgcc
     2161 ttatatcccg cagaatgccg ggatacttct ggtgccctgt tgccgtggcg ggtctggttt
     2221 aaccgtgggt aatgacggca cgttcagcga aacgtctggc gcatcggcaa attcagcccg
     2281 ctggggagtg ggtaaaccgc tgtatcagga ttttctcttc cgtacaaaag cggcgctgtt
     2341 gaagaacccg aaaaacaggc ttctggccgt ggtatggatg cagggggaaa atgatcttgc
     2401 ggacggcagt cagcagcaca gcggcctgtt taccgctatg gtgcagcagt tcagggctga
     2461 tatggcagca tattctgcac agtgcgttgg cggaagtgct ggctcggtgc cgtggatttg
     2521 cggtgacacc acgtattact ggaagaatct taacgccgat aaatatgagg cggtatatgg
     2581 cggttacaag ggcagggaag cacagaatat tttctttgtt ccttttatga cggacagcag
     2641 tggtgcaaac acatccacca acaatccgtc agaagacccg gatattccgg catcaggata
     2701 ttacggtgca gcctcccgga cgtcggcaaa ctggacgtca gcagaccgag cgagccattt
     2761 cagctcatgg gcacgcaggg ggattatttc cgaccgtctg gcaacggcga ttctggtgca
     2821 tgccgggaga gccgctgaat tcattaccgg aaaacagcct gatacggtga agcccacagg
     2881 gccttccggt gaaggtccgg agagagaggc ggaagccccg gtcagtaacc gaaccctgat
     2941 gagtctgctg gcgtcaggcg aagacctggc atcacagggc tggcgctatt atcacaaacc
     3001 ggcgagcgga gacagtattg ataaaaacat cgctgaggct gtggtcagtg atgagggtgc
     3061 caccggtggt aaggccctgc aactgaacaa accggaaagc cacatctggt ttctggagca
     3121 tgatgcagcc gggcaggggg cagagttact gaagaagggc ggacgtgtga gcgtgcggtt
     3181 taaggtaccg ggctcactgg tacagaaccg gtttgccctg ggtatttact ggcagttgtc
     3241 gtccctgccg gagggtgtgt cgctggcgga ggaaggcaac gacatgctga tgtccttctt
     3301 cctgcagacg gatgcgacga acctgaacgc gatgtaccac aagacgcaga atgcgaagct
     3361 ggatacgttc ggggcatttg ataacgaatg gcacacactg tcttttgagt ttgccggaaa
     3421 caacagtatt caggtgacgc cggtacttga tgagaagcgg ggcacgccat tcacgctggt
     3481 gaaatctcag gcaacagggg tggcggataa acttctgctg actgatatca cgaaggtggc
     3541 aacgtatacg ctgctgattg acagcgtgaa ggtggaagtg aataacccgt aagcaggaaa
     3601 aaaaagaccg ccggggcagg gaaaacaagg agccagaacc ggcggcaaat gtcgtcatat
     3661 ccaaagcaaa acatgcagga cgctttttta accaacaggt attaacgatg tcaacaccat
     3721 atcaataatg gagatgaata atgacattta tacagcttat tttattgtat ttctgtacag
     3781 cagtttgtgt gctgt
//
LOCUS       DAZGXZ010000259         3739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000259 DAZGXZ010000000
VERSION     DAZGXZ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.260"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..191
                     /locus_tag="ACHKJF_005280"
     CDS             <1..191
                     /locus_tag="ACHKJF_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952460.1"
                     /translation="NAEKYNEWARKFSEQIFKITGNENAAKNELEPWTPEGADPNYCW
                     REVDPVDAANEDMSYHND"
     gene            224..442
                     /locus_tag="ACHKJF_005281"
     CDS             224..442
                     /locus_tag="ACHKJF_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP0952461.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMIPISPLILVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            444..809
                     /locus_tag="ACHKJF_005282"
     CDS             444..809
                     /locus_tag="ACHKJF_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFP0952462.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            806..1150
                     /locus_tag="ACHKJF_005283"
     CDS             806..1150
                     /locus_tag="ACHKJF_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952463.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELSGAERTDKRGGYFTMTWPEALTTVGIAMA
                     VALVVYSICRWG"
     gene            1362..1517
                     /gene="hokD"
                     /locus_tag="ACHKJF_005284"
     CDS             1362..1517
                     /gene="hokD"
                     /locus_tag="ACHKJF_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0952464.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1684..1956
                     /locus_tag="ACHKJF_005285"
     CDS             1684..1956
                     /locus_tag="ACHKJF_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952465.1"
                     /translation="MAQHIKSHNSEVDPTIKRGRRSHVFKTDWFQHPPCTEEQAEWLI
                     QCYRRRGYEFEKALSFDRRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            1958..3007
                     /locus_tag="ACHKJF_005286"
     CDS             1958..3007
                     /locus_tag="ACHKJF_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0952466.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSVFHNGRILVEPEPKNMRG
                     LPSGVVPAVRQPLAEDKTLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDQLAQQNLVAWMIDVIRHAISGTQERE
                     LSLAELSWWAACNQVVDALPEAVARRSLGLPAEKIRSVYRESDIVPGEQTAISILKQR
                     TKNIALPLHVHQQQNPPQKKTVVSIAVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGVGTKAHDIFTLPLCREHHNELHADPLKFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            3020..3382
                     /locus_tag="ACHKJF_005287"
     CDS             3020..3382
                     /locus_tag="ACHKJF_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0952467.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     PVSGGRLEIRITEVGVHE"
     gene            3375..>3739
                     /locus_tag="ACHKJF_005288"
     CDS             3375..>3739
                     /locus_tag="ACHKJF_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0952468.1"
                     /translation="MNSQYLQFVREQLMIATADLSGSTKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIALVSPVEFATSSWRRAVLELEEHQK
                     AWLLWCYSGNVCWEDQVTIT"
BASE COUNT         1009 a          858 c         1017 g          855 t
ORIGIN      
        1 ttaatgcgga aaaatataac gagtgggcaa gaaaattctc tgagcagatt tttaaaatta
       61 ctggcaatga gaatgcggca aaaaatgaat tagaaccgtg gacgcctgaa ggagccgacc
      121 caaattattg ctggagggag gttgatccag ttgatgctgc aaatgaagat atgagttatc
      181 acaacgatta atgtcaggag gccgcccgaa agggcggtaa tgaatggtca cattatttag
      241 aaaaaaatat ccgcgaaaga gtagaacaac agaatttctg tttctcattc tgtttatcgt
      301 gttgatgata ccgatatccc cgttaatcct agtctggata atcggaaaaa taattgagcc
      361 agttattgaa ttgtataacg acgtggtatg ggcgtcattc aacacactgc acaataaaat
      421 taatccgtat aaggaaagct gatatggcac tgacgaaaaa acaacgcgca gaactgcgca
      481 tgaagttcgg tggtcgctgt gcttattgcg gctgcgaact tggcgaaaag tggcatgcag
      541 accatgtaaa accggtcatt cgttttgatg gaaatatgct tcaccaggaa cgtgacgata
      601 tatccaacat ggttccggca tgccacccat gcaatctgca caagcattgc agtagtctgg
      661 aagattatcg gcgaattatc agtgatggtc gtcgtgaatt ccttgcgtcc gggaaaggca
      721 aagcgctggt tcgtatggga ttggttgaaa tgaaatctga cccggttgtg ttctggtttg
      781 aaaaatatca agaaggggct acggcatgac gacttttacc agagagcagt taatagctca
      841 cgcagaggag actattgaag cacagagact gtgcataccg ggcacaatcg accatgacat
      901 catccgcaca tataagatgg atattgctgt tctggaaatc gcactggtat cgctggcagc
      961 agagccagcc ggtaaattgc atgaatacaa accagtggga tatcagcgtc tggtcgatga
     1021 gttatctgga gcggaacgga ctgataagcg tggaggatat tttacgatga cctggcctga
     1081 agcattaaca acggtaggaa ttgcgatggc ggtggcgctg gtggtgtatt cgatttgccg
     1141 ctggggataa aaacggtttg cgggaaaagg agagttaagt agaattgcag cgggtgcttg
     1201 aggctatctg tctcaggcat gaacaccaaa aggcagatag agaaaagccc cagttaacat
     1261 tacgcgtccg gcaagacgct taacattaat ctgaggctca atctatgaac ggcaaatcta
     1321 ggttagcctc ttacgagccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga
     1381 tgttaatcgc cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga
     1441 aagacctctg cgaggtacga atccgaaccg gccagacgga ggtcgctgtc ttcacagctt
     1501 acgaacctga ggagtaagag accaggcgag ggagaaatcc ctcgccacct ctgatgtgtc
     1561 aggcatcctc aacgcacccg cacttacccc gtttcggcag actttgtttt ttcctggcat
     1621 tctggtttac aattcgcacg tcagcctgaa caactggcac ctgctgcgtc accggagaac
     1681 ccgatggcgc aacatataaa atcccacaat tctgaagtcg atccgaccat taagcggggg
     1741 cggcgttcac acgtatttaa aaccgactgg ttccagcatc ccccatgcac tgaagaacag
     1801 gccgaatggc tgatccagtg ttaccgcagg cgcggatatg agtttgagaa agccctcagc
     1861 ttcgatcgtc gtcactggat aatctccgtc aggctccctt attccgaacg cccaccgcgt
     1921 ccgtcccgca cattccagca gcgcatctgg aggtaacgtg cgggtattac ttcgacctgt
     1981 tctggtaccg gaactcggtc tggttatcgt taagccaggc cgtgaatcaa tgtcagtatt
     2041 ccataacggc agaatattgg tggagccgga accgaaaaac atgcgcggtc tgccgtccgg
     2101 agtcgttcct gccgttcgcc agccgctggc agaggataaa acattactgc catttttcag
     2161 cgatgagcgg gttattcgtg cagcaggtgg tgcaggtgca ctgtctgact ggttattacg
     2221 tcacgtgaaa tcctgccagt ggccacacgg cgattatcat cacagcgaaa ccgtcattca
     2281 ccgttatggt accggcgcga tggtgttgtg ctggcactgt gacaaccagc tgcgcgacca
     2341 gacatcagaa tcactcgatc aacttgctca gcagaatctg gttgcctgga tgattgatgt
     2401 catccgtcac gcaataagcg gtacgcagga gagggagtta tcgctggccg aattatcctg
     2461 gtgggcggcc tgcaatcagg tggtggatgc actacctgag gcagtagcgc gtcgttcgct
     2521 gggattacca gcggaaaaaa tccgctccgt ataccgtgag agtgacatcg taccgggaga
     2581 acagacagcc atcagcatac tgaagcagcg cacaaaaaat attgcgctgc cacttcacgt
     2641 ccaccagcaa caaaatccac cacagaaaaa aacggttgtc agtatcgccg ttgatccgga
     2701 gtctcctgaa tcgttcatga ggcggcctaa acgtcgccgt tgggttaatg agaaatacac
     2761 gcgctgggta aagacacagc cgtgtgcgtg ttgtggtaag ccagctgacg atccacatca
     2821 cctgattggt catggtcagg ggggagtggg aacaaaggcc cacgatattt tcacgctacc
     2881 gttgtgccgg gagcaccaca acgaacttca tgcagacccg ctgaagtttg agaaaaagta
     2941 cggctctcag attgagttaa tttttcgttt tcttgatcac gcctttgcga ctggcgtgct
     3001 cgggtaaaag aggtgactga tgctcataga tttggtttta ccttacccgc cgacggtgaa
     3061 cacttactgg cgacgccgtg gcagcacata ttttgtatca aaagccgggg agcgttatcg
     3121 ccgggcagtg gcgcttattg ttcgccagca gcgactgaaa ttaagcctgt ccggacggct
     3181 ggcaataaaa attattgcag agccaccgga taagcgccgc cgtgacctgg acaatattct
     3241 gaaagcaccg ctggatgcgc tgacgcacgc ggggttgtta atggacgatg agcagtttga
     3301 tgaaatcaat attgtacgtg gccagccagt atctggtgga cggctggaga taagaattac
     3361 agaggtgggt gtgcatgaat agccagtatt tacagtttgt tcgtgagcaa ctcatgattg
     3421 ccactgcgga tctcagtggg tcgacaaaag gccagctgga agcctggcag gaaaatgccc
     3481 tgttcgatac agggcgttac agacgtaaaa aaattcgtta ccgcgatgag gtaaccggaa
     3541 aaatgatcac gcgggataat ccaccgatcc cgggcaaaca atcactggcg aaagggacgt
     3601 caattgctct ggtcagtccg gttgagtttg caacatcatc gtggcggcgt gccgttctgg
     3661 aactggaaga acatcagaag gcgtggttac tgtggtgcta cagtggaaat gtttgctggg
     3721 aagatcaggt caccataac
//
LOCUS       DAZGXZ010000260         3483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000260 DAZGXZ010000000
VERSION     DAZGXZ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.262"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(123..389)
                     /locus_tag="ACHKJF_005289"
     CDS             complement(123..389)
                     /locus_tag="ACHKJF_005289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952469.1"
                     /translation="MLLTSRRLFAGMPGAQATARQGRRDRSGRCKPGVFTHRTVATAR
                     NRASGFGGDWRVRQQLPVGVGPDDRMPPGSREPKVRPADRTTLQ"
     gene            954..1184
                     /locus_tag="ACHKJF_005290"
     CDS             954..1184
                     /locus_tag="ACHKJF_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952470.1"
                     /translation="MTVSIVSPSAAAVKPRRHTRFRREDIPAPEIDPVLKAFGRHIAR
                     SFRRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            1236..2597
                     /locus_tag="ACHKJF_005291"
     CDS             1236..2597
                     /locus_tag="ACHKJF_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP0952471.1"
                     /translation="MTLSAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPEQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDRISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            2644..3207
                     /locus_tag="ACHKJF_005292"
     CDS             2644..3207
                     /locus_tag="ACHKJF_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0952472.1"
                     /translation="MHSQLRERIRLMRARLDNARPVIEIPAEFQLFVTPAPVCDRLVT
                     LAEISNHDHILEPSAGTGAILRAIRDTAPGAMCDAVEINNGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDICEELPRGTFAYTDVPTMIIRLRA"
     gene            complement(3293..>3483)
                     /locus_tag="ACHKJF_005293"
     CDS             complement(3293..>3483)
                     /locus_tag="ACHKJF_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952473.1"
                     /translation="TGQHCSSQNQSGRKKKSSSSSSSSSSSSSSSRNNNIISNRQMTG
                     LKRQKPPSERPRNERGGA"
BASE COUNT          765 a          979 c         1010 g          729 t
ORIGIN      
        1 ctggcgcgtc gcccgttccc cttcccgttt gtgatgtgtc tgactcagga gtgtttcttt
       61 cttatgttat ggaactgttc ctgatgttgt tgttgttctt ttctctgctg ctctgattct
      121 gactactgca gtgttgtcct gtctgccggg cgcaccttcg gctcccggct gcctggcggc
      181 atccggtcgt caggcccgac tccgacaggc agctgctggc gcacccgcca gtctccaccg
      241 aacccgctgg cgcggtttcg ggctgtcgct acggtccgat gcgtgaaaac ccctggcttg
      301 cagcgaccgg aacggtcgcg ccgcccttgt cgcgccgtgg cctgcgctcc tggcatcccg
      361 gcaaaaagac gccgggatgt aaggagcaac gccgtttcgc aaaccgcgct tcacgtcgtt
      421 gcagcgggtc cctgctcatt gcctgccggg tgccacacac ccgtatgagt ccctgtcgcc
      481 gggggcatag acagccacaa cctgccgcac tgactgtcca gggtaaatga acagcccaca
      541 aaatttttta cccaaaaccc ggtaaaaaat tttgcgttct gcccaggaca ggtgcgtcag
      601 gccgtggcag tgatgcccct tgcggcgaag gttctcaaac gggtgaagtt cataccacag
      661 ggaatacagc agcaggttcc cgcgaaacgt caaaaccgcg gctcccgaaa ctcaacgggt
      721 gatgttcttt aaaaatcagg atttatttca ggcgccggca atacgcattt atgtgttctg
      781 tcagcatcct gctggcaggt aatgagaacg caggaacaga aggaggaata caccggaaag
      841 cgtcagatgc agatatgaaa aaagcaggtc agggacctgc ttaatccacg ccgaagagac
      901 tgtgcaacca gtctttaagg cactgttacc aacctgtcat aaggaatcca tttatgactg
      961 taagcattgt ttcaccttcc gcagcggctg taaagccccg tcgtcatacc cgtttcagac
     1021 gcgaagacat ccccgcgccg gaaattgacc ccgtgctgaa agccttcggg cgacacattg
     1081 cccggagttt tcgccggggg cgtggtgtac acatccccgc catgaaaaac acggccttcg
     1141 ggcaggtgct ccgcacactc gaactgaaac gcgcttttaa ctgatgaggt gagccgcccc
     1201 gcgaggggcg gcaacagacg gaaggagaac attttatgac attatcagca atcaccatga
     1261 ccgccccgga agccgccagc ccggtgcaga tgtaccgcgc gacctactca ccggatgata
     1321 acaaacttcg cctgtatgcc gtgtcacgcc tcgacccgga gacgtataaa aaagtgcatg
     1381 atgctggttt ccgctgggca ccagaacagg cgctgtttgt tgcgccagcc tggacaccgg
     1441 gccgggaaga cgtgctcctc tcacttgccg gagagattga ggatgaagac agcacgctcg
     1501 ctgaacgtca ggaagcacgg gcggagcggt ttaccggata cagcggaaag cgggccagtg
     1561 aatccgcaca ggcacttgat gaagtggaaa gactggccgc gatgatcccg cccggtcagc
     1621 ccattctcgt ggggcatcac agcgaacgcc gcgcccgtcg tgatgcgcag cgtattgaaa
     1681 acggcatgaa acgtgccgtg atgctctttg aacgtgcgga atactgggaa gaacgggcgc
     1741 ggtcggcact gcttcacgcg aagtataaag aacgtccgga cgttcgctgg cgtcgtatca
     1801 aaaaaatcga agctgatttg cgcaaggctg aaaagaccat cgcgcagtcg cagaaatatc
     1861 tgacgatgtg gcgggctgaa tcgctggatc tgaatatggc aaaactcatc agcagtcatg
     1921 accgtatcag tgcctgtttc ccgctggata cgtatccgcg cccggcagaa aaaagccagt
     1981 atgaaggaag tcgatcgtta tggtcggccc tggatgatga catcatcacc acggagcagg
     2041 cccgcgaaat tgcgatccgc tgtcatgaac ggcagattca gcatcagcaa cgctgggtta
     2101 accactatca gaaccgcctg atctatgagc gtgccatgct ggacgaaagc ggcggcgtgg
     2161 ttacccggac acaggatttt gagccgggcg gacaggtttt cagccggggc gagtggctga
     2221 ccatcatccg cgtgaacaaa agcaacgggg cggtgagttc agtcacaacg ccgaattaca
     2281 gtttcctcgg gtacagcggc acgatgaaag tgacgcccga tcgcatcacg gactacaagg
     2341 caccatcggc agaagaggct gccgtcgcca gtcaggccgc gaagcgtccg ccggtagtca
     2401 actatccggg ggaaggtttc cgggaaatga caaaggcaca gtgggccgcc ctgccccggg
     2461 actgtaaggc cgtgcgcagt gtggcagaag cagaagacca cggggcatac cgctaccgcc
     2521 gcacaatgga caataatttc cgcctggtga atgtgtatat caccgacatg aaaatcacgg
     2581 aaatcccgca aaaataagac ttaccccccg gagaaatccc cgggggatga cggagacaat
     2641 gacatgcaca gtcagttaag ggagcgtatc aggctgatgc gcgcaaggct ggacaatgcc
     2701 aggccagtta tcgagattcc ggcagaattt cagctttttg tgacacctgc cccggtctgc
     2761 gatcgcctgg tgacgctggc ggaaatcagc aaccatgatc acattctgga accctctgcc
     2821 gggaccggag ccatactgcg ggcgattcgg gataccgcgc cgggggccat gtgtgatgcg
     2881 gtggagatca acaacgggct ggtccggtat ctgcgggaaa attttaacgg tgtcagggtt
     2941 cagtgtggtg acttcatgga atggcagccg gtgcagtatt acagccgtat tattatgaat
     3001 ccgccgttca gccacgggca ggatatccgg catatcctgc gggctttttc cctgttgcgt
     3061 cccgggggcg tgctggtcgc tgtttgtctc aacgggccgc gccagcagga gaagctgtta
     3121 ccgttttctg acatctgcga ggagctgccg cgcggcacgt ttgcttatac cgatgtcccg
     3181 acgatgatta ttcgtttgcg tgcctgatga cacagggctg gcgggaggat aaatgcccgt
     3241 ctgtccgcgc cgggaaaaaa ccgcgcgccg gtggcgctga aaggggccgc gctcacgcgc
     3301 cgcccctttc attacgcgga cgttctgatg ggggcttctg tcttttcaga cctgtcattt
     3361 gtctgttact gatgatgttg ttgttcctgc tactgctact gctactgcta ctgctactgc
     3421 tactgctact gctcttcttt ttcctgccgc tctgattctg actactgcag tgttgtcctg
     3481 tct
//
LOCUS       DAZGXZ010000261         3474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000261 DAZGXZ010000000
VERSION     DAZGXZ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.263"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHKJF_005294"
     CDS             complement(<1..191)
                     /locus_tag="ACHKJF_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952474.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYS"
     gene            432..2078
                     /gene="cka"
                     /locus_tag="ACHKJF_005295"
     CDS             432..2078
                     /gene="cka"
                     /locus_tag="ACHKJF_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFP0952475.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDELQKKSNQLDADISKL
                     NSYKNTLQSKIGDVNKQKEAEEKARENAEVAEHETLNEEKQAVVEAEKRLDEAKAELA
                     KAESDVQSKQATVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLQRQLEAKQAEYSAV
                     ENELKNAVSFRDGKAAEVKEAEQKLKEAQDALEKSQIKDAVDTMVGFYQYITEQYGEK
                     YAKIAQDLAEKSKGKKIQGVDEALAAFEKYKNVLDKKFSKVDRDAIFNALESVNYDEL
                     SKNLTKISKSLKITSRVSFLYDVGTDFKNAIETGNWRPLFVTLEKSAVDVGVAKIVAL
                     MFSFIVGVPLGFWGIAIVTGIVSSYIGDDELNKLNELLGI"
     gene            complement(2075..2365)
                     /gene="cki"
                     /locus_tag="ACHKJF_005296"
     CDS             complement(2075..2365)
                     /gene="cki"
                     /locus_tag="ACHKJF_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HFP0952476.1"
                     /translation="MYLKYYLHNLPESLIPWILILIFNDNDNTPLLFVFISSIHVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
     gene            2389..2520
                     /locus_tag="ACHKJF_005297"
     CDS             2389..2520
                     /locus_tag="ACHKJF_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0952477.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(2719..2862)
                     /locus_tag="ACHKJF_005298"
     CDS             complement(2719..2862)
                     /locus_tag="ACHKJF_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0952478.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDT
                     QNS"
BASE COUNT         1012 a          610 c          865 g          987 t
ORIGIN      
        1 gagtaagcct gggtgggatt cacaccatta ctgatttcac tgaccacatg acgctgatca
       61 gccagtgtca gcgtgtaaac ctgtttctgg atgaggtcac gggctttgtc atactcatat
      121 ccggcagcaa caagggtgtc gagaagtcgt ttgtctgttt tgttcagttt atggcggggt
      181 gatttgctca catcgttctc cgttttttca tgatccaaca gttttatatc tcatcctgat
      241 ttgctccggt gttctgtttc gacgttctca atgcctgcct ccccatagtg tgatccttat
      301 tgcaaaaatg aaaatccatg ctcttgacac ggacaatgct gagtagtagg tttcaactgt
      361 acataaaacc agtggttata tgtacagtat attgttttta atttattgtt ttaattatca
      421 aagaggaatt tatggctaaa gaactaagtg gatatggccc aactgctggt gagtctatgg
      481 ggggaacagg ggcgaatctg aatcagcaag gtggaaataa taatagtaat agtggggttc
      541 actggggtgg tggctctggg catggtaata atggtggtca gggaaattca aattcatctg
      601 gttctacatc aactgtgatg aaaaccggtg agtcatatct gactccgtgg ggtgatgttg
      661 tcattaataa tgatggcctt ccggtgatga atggtattgt tatgacagaa gagaattcaa
      721 ctctggttga taatccattt ggaggggtct ccagagttct taattcttta attagtgata
      781 tgccatcact ttttgcagaa agctcaggaa ataacaacaa taacactgct tcagttaata
      841 ctgcaccaac gaatgctcag gtcagtgata tggataagag ttcgaaggtg gtgggtaatg
      901 taattaatga aaaacaaaag cagaagaata aaattgctac acagattagt gaaaaacaga
      961 agaaaatcga ggagatgaaa aaagtattca agcatcattc ttatcatggt atcactgatc
     1021 ttgagaggga tgttgatgag ttgcaaaaaa aatcaaatca gcttgatgca gatattagca
     1081 aacttaattc ttataaaaat acattgcaaa gtaaaattgg tgatgtgaat aaacagaagg
     1141 aagctgaaga aaaggctcgt gaaaatgcag aggtcgctga acatgaaact cttaatgaag
     1201 agaaacaggc ggtggtggaa gcagagaagc gtttagatga ggcgaaagct gaactggcga
     1261 aggctgaaag tgatgtacag agtaagcaag ctactgtttc cagagttgca ggagagcttg
     1321 aaaacgctca gaagagtgtt gacgtgaagg tcaccggatt tccgggatgg cgtgacgttc
     1381 agaaaaaact acagcgtcag cttgaagcta agcaggctga gtactccgca gttgaaaatg
     1441 aacttaagaa tgctgttagt ttcagggatg gtaaggccgc tgaggttaaa gaggcagagc
     1501 agaaattaaa agaagctcag gatgctcttg aaaagagcca gataaaagat gctgttgata
     1561 ctatggttgg attttatcaa tatataaccg aacaatatgg agaaaaatac gcaaaaattg
     1621 ctcaggattt agcggaaaag tctaaaggaa agaaaattca aggtgttgat gaagctctgg
     1681 cagcatttga aaagtataaa aatgtactgg ataagaaatt cagtaaggtt gatcgggatg
     1741 ctatttttaa tgcgttagag tctgttaatt atgatgagtt atctaagaat ttaacgaaga
     1801 tatcaaagtc gcttaaaatc actagtcgag tatcttttct atatgatgtt ggtactgatt
     1861 ttaaaaatgc aatagaaact ggtaactggc gacctttgtt tgtcacgctg gagaaaagtg
     1921 cggtagatgt aggtgtggca aaaattgtgg cattaatgtt tagttttatt gttggtgtgc
     1981 ctcttggttt ctgggggatt gcaattgtca caggtattgt ttcttcttac ataggggatg
     2041 atgagttgaa caaacttaat gagttgttag ggatttaatt tctctttaga gtgtaatata
     2101 tgaaacttgg caatcctact gggattgcca ttaataaata aaacaatgca gataacttgc
     2161 ataagtacca gggctctttt tttaactttg tattttcttt gatatatcta gatatggtta
     2221 atttagagta tggatatagc aatacatgta ttgatgatat aaagacaaat aacaaaggag
     2281 tgttatcatt gtcgttaaat attaaaataa gaatccatgg tataagtgat tcaggtaaat
     2341 tatgtaggta gtattttaga tacataattt ttcttttgtt ggaggtgtat gaaaatagta
     2401 atttttctaa ttacaatatt attcttgtca ggatgtcaag caaactatat tcgtgatgta
     2461 caaggaggaa ccgttgcacc atcttcgtct tctgaactga cggggatcgc ggttcagtag
     2521 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     2581 caaaaaaacc accgctgcca acggtggttt gtttgccgga tcaagagcta ccaactcttt
     2641 ttccgaaggt aactggcttt agtggagcac agataccaaa tgctgtcctt ccagtgtagc
     2701 cgctataagg cttcaacttc aagagttctg cgtatctaaa tccactatcc agttaccaat
     2761 ggctgctgcc agtggcgtta agtcgtgtct taccgggttg gactcaagac gatagttacc
     2821 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     2881 aacgacctac accgaactga gataccaaca gcgtgagcta tgagaaagcg ccacgtttcc
     2941 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3001 gagggagctt ccggggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3061 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3121 ctgtggcgcg gccttttccg ggttctcgtt ttttgcgcat atgtttcttt cggcgttatc
     3181 ccctgattct gtggataacg gtatcaccgt ctttgagtga ggtgactccg ctcgccgcag
     3241 ccgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagtgtacc ggaagctgca
     3301 ttttctcctt acgcatctat gcggcatttc acacccggca tggtgcactt ttcatacaat
     3361 ccgcactgat gccacatgat tacgccagta tacactccgc tatcgttacg tgactgggtc
     3421 atggctgcgc cccgacgccc gctaaagccc cctgatgccc tgacgggctt tagc
//
LOCUS       DAZGXZ010000262         3464 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000262 DAZGXZ010000000
VERSION     DAZGXZ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.264"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(24..671)
                     /locus_tag="ACHKJF_005299"
     CDS             complement(24..671)
                     /locus_tag="ACHKJF_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0952479.1"
                     /translation="MIQISNQQKLVFAWGVVLLVVRTTNLLSLFVEWVKLFTDKVTRG
                     GKMKKWMLAICLMFINEICQATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINP
                     VTGYGSGLMQVDSQHFNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAV
                     GAYNAGFRKTERQNQRRLAYASEVYRIYTGIKSSKGIRIPVTKKSLPEINSVQNN"
     gene            956..1342
                     /gene="traM"
                     /locus_tag="ACHKJF_005300"
     CDS             956..1342
                     /gene="traM"
                     /locus_tag="ACHKJF_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFP0952480.1"
                     /translation="MAKVQAYVSDEIVYKINEIVERRRAEGAKSTDVSFSSISTMLLE
                     LGLRVYEAQMERKESSFNQTEFNKVLLECVVKTQSSVAKILGIESLSPHIAGNPKFEY
                     ANMVEDIREKVSIEMDRFFPKIDNEE"
     gene            1531..2220
                     /locus_tag="ACHKJF_005301"
     CDS             1531..2220
                     /locus_tag="ACHKJF_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HFP0952481.1"
                     /translation="MCALDPRERPLNSQSINNYILNVQDIYKNSPVPVCVRKNRKILY
                     ANGAYIELFSREDKPLSGESYIRQHVEIFLSSLEIECQSLGHGSAFCRRFNFHGEIYQ
                     IRMENVSFYNDESVVLWQINLFPDYLFFCMKNEGYYNRDSYVQSVIANMSAKSLVVFC
                     FYILGYKHINIAKELEITEVASKKRIKKTNAEINKRFGSFDLFRNKCITTGVMNEILT
                     IVSEYMGVKKM"
     gene            2308..2535
                     /gene="traY"
                     /locus_tag="ACHKJF_005302"
     CDS             2308..2535
                     /gene="traY"
                     /locus_tag="ACHKJF_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFP0952482.1"
                     /translation="MRRRNARGGTSRTVSVYLDEDTNNRLIRAKDRSGRSKTIEVQIR
                     LRDHLKRYPDFYNEEIFREVTEERESTFKEL"
     gene            2570..2923
                     /gene="traA"
                     /locus_tag="ACHKJF_005303"
     CDS             2570..2923
                     /gene="traA"
                     /locus_tag="ACHKJF_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFP0952483.1"
                     /translation="MNTSVSVPGAFTPVKKKSSFFSKLNLLRLVRAVIPAAVLMMFFP
                     QMALAAGGQDLMQSGNDTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFLAGFA
                     IISVFIAVGMTVVGL"
     gene            2938..3249
                     /gene="traL"
                     /locus_tag="ACHKJF_005304"
     CDS             2938..3249
                     /gene="traL"
                     /locus_tag="ACHKJF_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFP0952484.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTVLLRGIFHNVPDSCFRQWI
                     K"
     gene            3271..>3464
                     /locus_tag="ACHKJF_005305"
     CDS             3271..>3464
                     /locus_tag="ACHKJF_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFP0952485.1"
                     /translation="MEHGARLSTSRVLAIAFIFMSLLIVLSLTVNVIQGVNNYRLQNE
                     QRTAVTPMGFNMPFAVSQNSA"
BASE COUNT         1026 a          624 c          748 g         1066 t
ORIGIN      
        1 ggtatctgac aatgtctgta agattaattg ttctgcacgc tgttaatttc cggaagtgat
       61 ttcttcgtga ccggaatccg gataccttta ctgcttttta tcccggtata aatccggtaa
      121 acctctgatg cgtaggcaag acgtctctgg ttctggcgtt cggtcttcct gaatccggca
      181 ttgtatgcac caacggcctc ccaggagacg ccccattttt taaaggctat tgccagataa
      241 taagcaccgg tataaatgtt catgcaggga tctgttgtca gatgttccgg tttaattcca
      301 tagcgggcca gttcgttaaa atgctgggaa tctacctgca tcagtccgct gccatatccc
      361 gttaccggat taataccgat ggcattaacc cggtaacggg attctttcca tgatattgct
      421 ctcagtaaat caggatctat tttgtaatcc cggcctgcaa gatcaaagca atcagtggcc
      481 tggcagatct catttataaa catcaggcag atggctaaca tccatttttt catttttcca
      541 cctctggtga ctttatccgt aaataattta acccactcca caaaaaggct caacaggttg
      601 gtggttctca ccaccaaaag caccacaccc cacgcaaaaa caagtttttg ctgatttgat
      661 atttgaatca ttaacttatg ttttaaataa tgtattttaa tttattttac attataaaaa
      721 ggattcattg gtgaatcgta tatgattcac caatgaattt aacacaactc atagatgaat
      781 cagtaataat aaaacaacga gttaaagaga attgactcta gattcaattc gtgatgtgat
      841 ttgctgcatg aatcgtaatt ttcgtaactt gcgtcacgat tcgattcatc gatgaattgt
      901 cattttttat atgtgaaata aatttgatgc gataagaaaa taaagaggta tacatatggc
      961 gaaagtgcag gcttatgtca gtgacgaaat tgtctataaa ataaatgaaa ttgtggaaag
     1021 aagacgtgca gaaggagcta aaagtacaga tgtaagtttc tcaagcatat ccactatgct
     1081 tcttgaatta gggcttcgtg tatatgaagc tcagatggag cgtaaagagt catcctttaa
     1141 tcagacagag tttaataagg tacttcttga gtgtgttgta aaaacacagt catcagtggc
     1201 taagatttta ggtattgagt ccctcagccc tcatatagca ggtaatccaa aatttgaata
     1261 tgcaaatatg gttgaagata tcagggagaa ggtatcaatt gagatggata gattttttcc
     1321 aaaaattgat aatgaggaat aaaaatgaaa gtttgatttc cttcaaataa aagaatattg
     1381 atctggttct atttgatgac aattaaccac ataaggagta tattaattat gtggttaatg
     1441 ccacgttaaa atttaaatga aaaatcgccg atacagggag gtcagatctc cctgtatcga
     1501 ctgtccatag aatccttaac ggaggttcct atgtgtgcgc tggatcctag agaaaggcca
     1561 cttaacagtc aatctataaa taattacatc cttaatgttc aggatatcta taaaaattcg
     1621 cctgtcccgg tttgtgtccg taagaacagg aaaatccttt acgcaaacgg agcgtatatt
     1681 gagctctttt ccagagaaga taaaccccta tccggagaga gttatatacg tcagcatgtt
     1741 gaaatttttc tttcatcact tgagattgaa tgccagtctc ttgggcatgg ttctgcattt
     1801 tgtcgtcgtt ttaattttca tggggaaatt tatcaaataa ggatggagaa tgtttcgttt
     1861 tataatgatg aatctgttgt tttatggcag attaacctct ttcctgatta tttgttcttt
     1921 tgtatgaaaa atgaagggta ttataataga gacagctatg tacagtctgt catagctaat
     1981 atgtcagcaa agtcattggt tgttttttgt ttctatatat taggatataa acatataaat
     2041 atagctaagg aactagaaat tacagaagtg gcatctaaaa agagaatcaa aaaaactaac
     2101 gccgaaataa acaaaagatt tggttctttt gatttattca ggaataaatg tatcacgaca
     2161 ggagtaatga acgaaatatt aacaatcgta agtgaatata tgggtgttaa aaaaatgtaa
     2221 taaatgatgg ctcgttttgg gcccattttc gacaggccat ttctgtgtaa tcattatttt
     2281 attactcggg aaacagaggt gaatttagtg aggaggcgta acgcgagagg tggtacaagc
     2341 agaacggttt cagtgtatct cgatgaagat acaaataacc gacttattcg ggcgaaagac
     2401 cgtagtggca gaagtaaaac tatagaagtc cagatccgtc tcagagatca tctgaagcgg
     2461 tatcctgatt tttataacga agagatattc agggaggtga cagaagagag agaatcaact
     2521 tttaaagaac tttagtttta atctttttac atacaatccg aggtaattga tgaatacttc
     2581 tgttagtgtt ccgggggctt ttacgcctgt taaaaagaaa tcatcttttt tctctaaact
     2641 gaacctgctt cgcctggttc gtgctgtgat cccggctgct gtgctgatga tgttctttcc
     2701 tcagatggcc ctggctgctg gcggtcagga cctcatgcag tccgggaatg acaccgtcaa
     2761 agccaccttt ggtaaggact ccagtgttgt taaatgggtc gttctggctg aagtgctggt
     2821 cggggcggtc atgtacatga tgaccaaaaa cgtcaagttc ctggccggtt ttgccatcat
     2881 atctgtattt attgctgtgg gtatgaccgt cgttggcctc tgacaggaaa taaaacgatg
     2941 tcgggagacg agaataaact taagaaatat cgtttcccgg aaacactgac caaccagagc
     3001 cgctggtttg gcctgccact ggatgaactg atccccgcag cgatctgtat tggctggggt
     3061 atcacaacat cgaaatatct tttcggtatt ggtgcggcgg ttctggttta tttcgggatt
     3121 aaaaaactga aaaaagggcg gggcagctcc tggttacggg atcttattta ctggtatatg
     3181 ccaacagtcc tgctgcgcgg tatttttcat aatgttcctg attcgtgttt ccggcagtgg
     3241 attaaataga actgatacct ggattgttat atggaacacg gtgcccgctt aagtaccagt
     3301 cgtgtactgg ctattgcttt tatatttatg tccctgctca ttgttctcag cctgactgtt
     3361 aacgtgattc agggggtgaa taattaccgc cttcagaatg aacagcgaac tgccgtgaca
     3421 ccgatgggat ttaatatgcc ctttgcggta tcacagaaca gtgc
//
LOCUS       DAZGXZ010000263         3447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000263 DAZGXZ010000000
VERSION     DAZGXZ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.265"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..470
                     /locus_tag="ACHKJF_005306"
     CDS             <1..470
                     /locus_tag="ACHKJF_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0952486.1"
                     /translation="LDNFNFRDCEFEKCRFVNCSIKNLKLNFFKLIDCEFKDCLLQGV
                     NAADIMFPCTFSLVNCDLRFVDFISLRLQKSIFLSCRFRDCLFEETDLRKSDFTGSEF
                     NNTEFRHSDLSHCDFSMTEGLDINPEINRILSIKIPQEAGLKILKRMGVVVGG"
     gene            1040..1705
                     /locus_tag="ACHKJF_005307"
     CDS             1040..1705
                     /locus_tag="ACHKJF_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6710 family protein"
                     /protein_id="HFP0952487.1"
                     /translation="MMFWNRNKKDKATIANEKKRFNHLLSVAEKLPVKALPDLIRAIV
                     RPVQSDFLLAVAEEGTDARPDMTPQEFFFKGLTQIQSYEKMKECEMNGADYPLSLASD
                     IVLPWPWSLPRYIDNVSRIGTHKGKPWKQDKNNHYVDLWLPWRIGFVHGGNHSITAGI
                     LAGEGSLIPEHVYDMSWMFELVRTDGNNWFVEGQKAEAVKSGRSAAVFEIGRLLVEGA
                     KER"
     gene            complement(1741..2040)
                     /locus_tag="ACHKJF_005308"
     CDS             complement(1741..2040)
                     /locus_tag="ACHKJF_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0952488.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLVENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDEASRVNILAVVGQRQNPRFIL"
     gene            complement(2030..2293)
                     /locus_tag="ACHKJF_005309"
     CDS             complement(2030..2293)
                     /locus_tag="ACHKJF_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFP0952489.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSAQLRQQLQGRYQELRNEL"
     gene            complement(2313..2450)
                     /locus_tag="ACHKJF_005310"
                     /pseudo
     CDS             complement(2313..2450)
                     /locus_tag="ACHKJF_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(2798..>3447)
                     /locus_tag="ACHKJF_005311"
     CDS             complement(2798..>3447)
                     /locus_tag="ACHKJF_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP0952490.1"
                     /translation="GSRRLYVISPSEEYKPLDALIAGKAKMEVIEQHWDEVLRLKASI
                     GAGLVPPSVILKKLAASPRQNRLNLALREMGRIERSIFICDWLLDTRLRRRSHAILNK
                     GESRHALARAIFLHQLGEFRNRAAETMAHRASGLNLVVNAIILWNTVYLSRAVDYIRS
                     QGIIIPEELLSSVSPLPWGHISLTGDYLWNEIDHPLERYRPLRTNRFNPKNFTFP"
BASE COUNT          881 a          792 c          860 g          914 t
ORIGIN      
        1 agcttgacaa ctttaacttt cgcgattgtg agttcgaaaa atgtcgcttc gtaaactgct
       61 caataaagaa tttaaaactt aattttttta aacttattga ctgtgaattc aaagattgtt
      121 tattacaagg agtaaatgca gcagacatta tgtttccctg tactttttct cttgtaaatt
      181 gtgatttgag atttgttgat tttattagtt tacggttaca gaaatctatt tttttatcct
      241 gtcgcttccg tgattgttta tttgaggaaa cagaccttcg taaatctgat tttactggtt
      301 cggagtttaa taatacagag ttcagacatt ctgatttgtc tcactgtgat ttttcaatga
      361 cagaaggtct cgatataaat cctgagataa acaggatctt atctataaag ataccacagg
      421 aagcaggttt aaaaatactt aaacgaatgg gagtggttgt agggggatga ggtgagtaga
      481 cccgccggtt gcccggacgg gcccctcctc agaaccggac ctgcggaact accgcatccg
      541 gctcccgaca gatcagtgtc cccacacata tttcagaaag aatggaacat tgactgagtt
      601 ctccatctcg atgggtatcc cagcattttg agaatcagat ccagcttttc tcatgtcatt
      661 gggtgtgggg tttgaagccc aatggagcgg aaacgtacgc taagaagcta actatatgat
      721 tttaagtcaa taaagtcgtg gcgttgttag gcaggtttac gttgatttga ttaccgagtt
      781 tattaacact gaaagtccct gaaaccgcat ggctactggc attgacggga agagtaaata
      841 aacccctcgt tttctttact cacctcttct gctcaaaaaa tgaacttagc gtacgatctc
      901 gtacggttgg gcctcaaacc cctggttgac cggctaacgc cgaagctttc gaagaatatg
      961 cacgtgcgcg tgacgtgaaa ctactacacc attagaatga aaaccatcat cctgatcacg
     1021 ataaggaggg ctacagacag tgatgttctg gaaccgaaac aagaaagata aagcaactat
     1081 agccaatgaa aaaaaacgct tcaatcatct gctttctgtg gcagagaaac tacccgttaa
     1141 ggcgctacca gatctcattc gcgctatcgt gcggccagta cagagtgatt tcttgctggc
     1201 ggtagctgag gagggaacag atgcccgccc ggatatgacc ccgcaggagt tcttctttaa
     1261 gggacttacc caaatacaat cttacgaaaa gatgaaagag tgtgagatga acggcgctga
     1321 ttatccgttg tcgctggcaa gcgacatagt gctgccgtgg ccttggagcc taccacgcta
     1381 tatcgataac gtttcacgca ttggaaccca taaggggaag ccgtggaagc aagataaaaa
     1441 caaccactat gttgacctgt ggttgccctg gcgtattggc tttgtgcacg ggggcaacca
     1501 ctccataacg gcgggtattc tggccggaga agggtcgctg atcccggaac acgtttacga
     1561 tatgtcatgg atgtttgagt tggtccgaac ggacggtaat aactggtttg ttgagggaca
     1621 gaaagccgaa gcggtgaagt cgggacggtc agctgcggtg tttgagatag gtcggctgtt
     1681 agtggaagga gctaaagaac gttgatcgat tctctgaaga tggagttcca agccggacac
     1741 tcaaagaatg aatcggggat tctgacgctg gcctacaacc gccaggatat tgacacgact
     1801 ggcctcatca ttcacttcat aaagtacatg ctgtccaagt aagctaatac ggcgaacccc
     1861 ttcaccatag cgttcatccg tttcatacag cctggggtaa tcagccaggc gatcggttgc
     1921 agtgatcaga cgctctgtca cgttccttgc atcccaaaga gatgcattct cgaccagata
     1981 ttcaaacaac gccgttatat cctcaatcgc acctggtcgc cattctattt cataactcat
     2041 tgcgcagttc ctgataacgt ccctgtagtt gctggcgcag ttgcgcggaa ctcattcccg
     2101 gccgcttatc ggcgcgggca gcggcaactt tcgctcggat ctgttcgtcg tattccgccc
     2161 gtgccagttc ttcattagcg aggcgttgca atagtgcaga cttgtctgta ctgccttctg
     2221 gaaaaatacg aggcaggctt tcggccagct cgcgcaggaa attggcgttt tcgtaatgtg
     2281 tggtgctatt cataattgac tccccctttc attcaataaa gggcaattgc aggttttttg
     2341 atgccacacg catgggtata acgagtcaga gtatcgatat tcgccccggt gatattgttt
     2401 cacaggcagg cgataaccgg atgcataatg ctcatttgcc tggcaatctg cgtgttgtca
     2461 ggttttatgc agagtatata acctttcaga cacagccacc aacatatgct tatgccgcgc
     2521 cagcggcatg gaaggcggca cgctgtggtt acatgtgata ccggagtaaa accgccgaag
     2581 cccggcgtca gccggtactg attgatagat ttcaccttac ccatcccttg ccctgccaga
     2641 ccatacccgt tttcagccat gagagagttt ctgtgcgcgg tcggagtggt cccgacgagg
     2701 gtttacccga agtcggggcg tatctccgcg ttagcgggcc gtcagggccg cttacgagcg
     2761 tgtaagggtt ccgtgggcat ttctgcgata atacacgcta aggaaaagtg aagttctttg
     2821 gattgaagcg attagtacga agtggcctgt atcgttcaag gggatggtca atttcgttcc
     2881 acaggtaatc tccagtgagc gagatatgac cccatggtag tggtgataca ctggacaaaa
     2941 gctcttccgg tataatgatt ccttggctgc gaatgtaatc aacagcccga ctgagataga
     3001 ccgtgttcca cagaattatt gcattaacaa caaggttgag gcccgatgcc ctgtgagcca
     3061 ttgtctcggc agccctgttc cggaattcgc ccagttggtg aagaaaaata gcgcgggcaa
     3121 gtgcatggcg gctttcgcct ttgttgagga ttgcgtgtga tctgcgacgt aaccgagtgt
     3181 caagcaacca gtcacagata aaaattgagc gttcaatacg tcccatttca cgcagggcca
     3241 gattgagcct gttttgtctc ggagatgctg ccagcttttt cagtattacc gatggtggta
     3301 ccagaccagc gccgatcgag gccttaagcc gcaaaacttc atcccagtgc tgttctatta
     3361 cttccatttt ggcctttccg gcgatcaggg catcaagtgg tttatattcc tcagaaggtg
     3421 aaataacgta caacctgcga ctgccga
//
LOCUS       DAZGXZ010000264         3386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000264 DAZGXZ010000000
VERSION     DAZGXZ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.266"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            41..1138
                     /locus_tag="ACHKJF_005312"
     CDS             41..1138
                     /locus_tag="ACHKJF_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0952491.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1328..1711)
                     /gene="quuD"
                     /locus_tag="ACHKJF_005313"
     CDS             complement(1328..1711)
                     /gene="quuD"
                     /locus_tag="ACHKJF_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFP0952492.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1797..1937)
                     /gene="ylcG"
                     /locus_tag="ACHKJF_005314"
     CDS             complement(1797..1937)
                     /gene="ylcG"
                     /locus_tag="ACHKJF_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0952493.1"
                     /translation="MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            complement(1934..2296)
                     /gene="rusA"
                     /locus_tag="ACHKJF_005315"
     CDS             complement(1934..2296)
                     /gene="rusA"
                     /locus_tag="ACHKJF_005315"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP0952494.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            complement(2293..2583)
                     /gene="ybcO"
                     /locus_tag="ACHKJF_005316"
     CDS             complement(2293..2583)
                     /gene="ybcO"
                     /locus_tag="ACHKJF_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0952495.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            complement(2576..2746)
                     /gene="ninE"
                     /locus_tag="ACHKJF_005317"
     CDS             complement(2576..2746)
                     /gene="ninE"
                     /locus_tag="ACHKJF_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0952496.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPEPKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            complement(2746..3201)
                     /gene="ybcN"
                     /locus_tag="ACHKJF_005318"
     CDS             complement(2746..3201)
                     /gene="ybcN"
                     /locus_tag="ACHKJF_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP0952497.1"
                     /translation="MNLPQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLINRGKTFATPAWVKDALKHTYLGYETKDLVDVVTGDITTI
                     QSLRHTSDLDIGEMYVFLCKVEAWAVNIGCHLTIPQSCEFQLLRDKQEA"
     gene            complement(3198..3299)
                     /locus_tag="ACHKJF_005319"
     CDS             complement(3198..3299)
                     /locus_tag="ACHKJF_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952498.1"
                     /translation="MRTHNPNSLLPPQMQKCTCDILYPAFDLCGGEA"
BASE COUNT          895 a          816 c          756 g          919 t
ORIGIN      
        1 acatcatttt ttaataatag ttcaaaaaaa agggctcacg atgaaaaaat taacagtggc
       61 aatttctgct gtagctgcat cagtactgat ggcgatgtct gctcaggcag ctgaaattta
      121 taataaagac agtaacaagc tggatctgta cggaaaagtt aatgccaagc actacttttc
      181 ctctaacgat gcagatgatg gtgatactac ttatgttcgt ctgggcttca aaggcgaaac
      241 ccaaatcaac gatcagctga ctggtttcgg tcagtgggaa tatgaattca aaggcaaccg
      301 cgctgaatct caaggttcct ccaaagacaa aacccgtctt gcatttgcag gcctgaaatt
      361 cggtgactac ggctcaatcg attacggccg taactacggt gtagcatacg acatcggtgc
      421 gtggactgac gttctgccag aattcggtgg cgatacctgg acccaaacag atgtgttcat
      481 gactggtcgc accactggtg ttgcaaccta tcgtaacaac gacttctttg gtctggttga
      541 tggtctgaac tttgctgctc agtaccaagg caaaaacgat cgtagcgatt tcgataacta
      601 caccgaaggt aacggtgatg gcttcggttt ctctgctacc tatgaatacg aagggttcgg
      661 tatcggtgca acttatgcga aatctgatcg taccgacact caagttaatg cagggaaagt
      721 tcttcctgaa gtatttgctt ccggtaaaaa tgcagaagtt tgggccgcag gtctgaaata
      781 tgacgctaac aacatttacc tggccactac ctattctgaa acccagaata tgactgtatt
      841 tgctgatcac ttcgttgcta ataaagctca aaacttcgaa gctgttgcac aatatcagtt
      901 cgatttcggt ctgcgtccgt ccgttgctta cctgcaatct aaaggtaagg atcttggagt
      961 atggggcgat caggacttag tcaaatatgt tgatgtaggt gcaacctatt acttcaacaa
     1021 aaatatgtct actttcgttg attacaaaat caacctgctt gacaaaaatg acttcactaa
     1081 agcactcggt gtaagcactg atgacatcgt tgctgtaggt ctggtttacc agttctaatc
     1141 tgattacgaa aaagatatgt tgcgggaggc tttgcctccc caacatataa gtggctccct
     1201 caagccactt cctttaggag cacaaccttg cttctaacta tataaacctt ctgttatata
     1261 ttacccttta tttttggggg cgttgcaacg ccccattttt aataattttt agtaaacaat
     1321 tggcatatta attagagtta ttaacaacga tatccatctc taaccggata tctaatgcca
     1381 ttaacatccc ttcaattatg ccctcagcct tctgtaacct tttcccgata taaccatcag
     1441 agcagcaatg cttacctgcc agtgacatga atgtcatacc gactacataa taatctacta
     1501 ataaatcgtg caaatcgctg ttgttctttt tcagacgggc catgcacccg caaatgatca
     1561 tcgcgtcatc gtcacaacat tgcgggcgag attttacttt tgaaggaatt aatcccttaa
     1621 aaccggcggc aatggacgac caggtcacat cttcatgatt attagccgcc cacgctcccc
     1681 aacgctcaag aaccatctga atatcacgca tcaacttact ccacaaaaat cagaccagaa
     1741 cgccaattac aagcaaaaat caacaaaaca gtattagttg attgttatct ctgacttcat
     1801 actcctgctc ctgtcagggt tttggcgtaa ttcttcagta ttcggtaatc ggtcaaaaca
     1861 gaaccgggga aacgatataa gcgcagacgc ccccagcggt ggcgaagaag ttctgccata
     1921 ttaaactcaa acatcattca ttccccattt cggtgatggt cagttccagc ctcccacctt
     1981 tggtaacagg catcttcaca acgcggtaat caacgacctg agcatcatcc agccagaaac
     2041 ctgctttagt gagtgcgtca aaagcggctt tttgcagatt atccaggtca cggcgacggc
     2101 gatccggcat gtggcactca atgcggattt tcacaggcat agccaggccg atatccagca
     2161 ttgcgttttt aatgattcgg gcgacgttat cgcggtatgc ctgcccctct gcgctgacgt
     2221 gcgtgcgccc gcgattatgg cggtaatagc gattattgct cggaggccag ggtaatgtga
     2281 tactgtaggt attcacgcct taataacccc ctctttcagc cagataacct gtgttctcgc
     2341 cataccttcc agcgcgcatt cttttgcata tgcagcatcg acaaaatgtg tgcggcggtc
     2401 gatttcgtcg tggcaggcag aacatgcaat ggtggcaatc aggtctggcg gtttgatacc
     2461 ggtaccgcac aatccagcca gccggatatg tgccagtaca gacgtttcag aattgccatt
     2521 acatacgcca gggattctta cctggcattc ccgaccacgc gctgcttttc tcaaatcagc
     2581 catgattcct ccttgctgcc agtcgcaacc attttttatc aaccaggcta gcggtatatc
     2641 cgagcagtgt tggtatttcg gatggcttcg gctcaggctt acgcttacga cgatttgata
     2701 ctctgtagat gtgtccgttc atgacacgaa taagcggtgt agccattacg cctcctgctt
     2761 gtcacggagc agctggaact cgcagctctg cggaatagtc aggtggcagc caatattcac
     2821 cgcccaggct tcaaccttac acaggaagac atacatctct ccgatatcaa gatcggaggt
     2881 atggcgtaac gactggatag tggtgatatc accggttacg acatcaacca ggtctttggt
     2941 ttcataaccg agatatgtgt gtttgagagc atcttttacc caagctggag tggcgaacgt
     3001 tttacccctg ttgatgaggt attcactgat ttcgctgtac cacatgtggc tgagtgcatt
     3061 ctgggaaaga ctgcgtttct cacgccacgg tttaagcacc atgcgaaagc atttgccctc
     3121 ctccagataa ggctggatct gccgaccgat agcggtgaag ttaccgcgat gtaatttgat
     3181 gccatcttgt gggagattca cgcttcacct ccgcagaggt caaacgctgg atacaatata
     3241 tcgcaggtgc atttctgcat ctgtggaggg agaagagagt ttggattgtg tgtgcgcata
     3301 aacgtccccg tttagcgcag aagtcaccgg agttgttcag gctccgatga catgattatg
     3361 gcgagttgat tatcgcaaat caaagg
//
LOCUS       DAZGXZ010000265         3352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000265 DAZGXZ010000000
VERSION     DAZGXZ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.267"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..141
                     /locus_tag="ACHKJF_005320"
     CDS             <1..141
                     /locus_tag="ACHKJF_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952499.1"
                     /translation="MKKGDAAEHAEFHMKDNRWVPGWMCTPCPQAETETTEYRDDQAE
                     AA"
     gene            210..644
                     /gene="psiB"
                     /locus_tag="ACHKJF_005321"
     CDS             210..644
                     /gene="psiB"
                     /locus_tag="ACHKJF_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0952500.1"
                     /translation="MKTELTLNVLQTMNAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            641..1360
                     /locus_tag="ACHKJF_005322"
     CDS             641..1360
                     /locus_tag="ACHKJF_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP0952501.1"
                     /translation="MSARSQALVPLSTEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            1357..1684
                     /locus_tag="ACHKJF_005323"
                     /pseudo
     CDS             1357..1684
                     /locus_tag="ACHKJF_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
     gene            1903..1983
                     /locus_tag="ACHKJF_005324"
                     /pseudo
     CDS             1903..1983
                     /locus_tag="ACHKJF_005324"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            1949..2050
                     /locus_tag="ACHKJF_005325"
     CDS             1949..2050
                     /locus_tag="ACHKJF_005325"
                     /inference="COORDINATES: protein motif:HMM:NF007279.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP0952502.1"
                     /translation="MIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK"
     gene            2143..2316
                     /locus_tag="ACHKJF_005326"
     CDS             2143..2316
                     /locus_tag="ACHKJF_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952503.1"
                     /translation="MAPFSLLTLWQHEQPDCQKGHFPPGTGTGMNVRLRRTFFSPAFS
                     VYGQLSEMTLPFP"
     gene            complement(2509..2682)
                     /locus_tag="ACHKJF_005327"
                     /pseudo
     CDS             complement(2509..2682)
                     /locus_tag="ACHKJF_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2982..3284
                     /locus_tag="ACHKJF_005328"
     CDS             2982..3284
                     /locus_tag="ACHKJF_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952504.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
BASE COUNT          734 a          892 c         1014 g          712 t
ORIGIN      
        1 atgaagaaag gcgatgccgc tgaacatgca gagttccaca tgaaagacaa ccgctgggtg
       61 cctggctgga tgtgtacacc atgtccacag gcagagacgg aaaccacgga gtaccgtgac
      121 gaccaggcag aagccgcctg accgccagtc ataaaccatt tatcacgccg ccccgccgga
      181 gacggggcgg cagcaaggga gataccgtga tgaaaactga actgaccctg aatgtattac
      241 agaccatgaa cgcacaggaa tatgaagata tccgggctgc cggaagtgat gaacgccgtg
      301 agctgactca cgccgtgatg cgggagctgg atgcgccgga taactggacg atgaacggcg
      361 agtacggcag cgagtttggc ggctttttcc ccgtccaggt ccgtttcacg cctgcccacg
      421 aacgttttca cctggcctta tgttcaccgg gcgatgtctc tcaggtctgg gtgctggttc
      481 tggtgaatgc cggtggtgag ccgtttgcgg tggttcaggt acagcggcgg tttgcaccgg
      541 aagccgtcag ccattcactg gcactggcgg cgtcactgga cgcgcagggg tacagcgtca
      601 gtgacatcat ccatatcctg atggcggaag gaggtcaggc atgagcgcac gttcacaggc
      661 actggttcct ctcagcacag agcaacaggc cgcatggcgg gcggtggcgg agaccgaaaa
      721 acgccgccat cagggtaaca cgctggctga atatccgtat gcgggggcgt ttttccgctg
      781 tctgaacgga agccgcagga tatcgctgtc tgacctgcga tttttcatgc cgtccctgac
      841 cgcagaagaa ctgcacggca accgtcttca gtggctgtat gccatcgatg tgctgattga
      901 aacacagggc gaggtttgcc tccttcccct tccaggcgat gcagcagaac gcttgttccc
      961 gtcggtgcgg ttccgtgtca gggagcgcag tcgccataaa tccgcactgg tcatgcagaa
     1021 gtacagccgt cagcaggcac gggaggcaga gcagaaagcc cgtgcgtacc aggcgctggt
     1081 cgcccaggct gaaatcgaac tggcgttcca ttcaccggaa acagtcggaa gctggcatgc
     1141 acgctggagt gaccgggtgg cagagcatga cctggaaacc ctgttctggc agtggggcga
     1201 gcgttttccg tcactggccg ggatggaacg ctggcagtgg caggatatgc cattctggca
     1261 ggtcatcgca gaggctggta tggcggcaag ggaggccggt catgccgtcc gtgagatgga
     1321 gcgatggatg gtgccgaaca aactccggga ggcggcgtga agtggcgtta ctcattgcgc
     1381 tggaaactgc cttatccctg tcctggcgag catgaactgg tttcagaagt gggtttcaga
     1441 agtggtgaag gcggggcagc ctgcaccggc atccgttatg tcacgctggg ttgccggagc
     1501 agggtatgcg gtctgtctgg attttatttc tgaccgcccg gtgcgccgct ggagcgaaga
     1561 acgtaaggct gcggtacggc ggcggaatct ggagaaacgt atcaacaggc atgcactact
     1621 gtttgcggat gaactgattg cccgtgaact ggctgaacga ccggattatt ttcagggaaa
     1681 gtgagtgtgg tcagcgcgca gggatatggg ctatgatgtg cccggcgctt gaggctttct
     1741 gcctcatgac gtgaaggtgg tttgttaccg tgttgtgtgg cagaaagaag atagccccgt
     1801 agttaatttt tcattaaccc acgaggcatc ccatatgtct gacaacatca ggatagcctc
     1861 ttaccgcgct ttgcgcaagg agaagaaggc catgaaacta ccacgcagct ctcttgtctg
     1921 gtgtgtgttg attgtgtgtc tcacactgtt gatattcact tatctgacac gaaaatcgct
     1981 gtgcgaaatt cgttacagag acggatacag ggaggtggcg gctttcatgg cttacgaatc
     2041 cggtaagtag caacctggag gcgggcgcag gcccgccttt tcagggctga tgctggtctg
     2101 actgcactga agcgccttta taaaggggct gctggttcgc cggtggcccc tttctccttg
     2161 ctgacattgt ggcagcatga acaaccagac tgccagaaag gccacttccc tccgggaacg
     2221 ggaacgggaa tgaacgtgcg cctgcggcgc acgttctttt cccccgcctt ctctgtttat
     2281 ggccagttgt cagaaatgac attacctttc ccgtgattat tctctttttc ttcctgctct
     2341 gattctgact actgcagtgt tgtcctgtct gccgggcgca ccttcggctc ccggctgcct
     2401 ggcggcatcc gtcgtcaggc ccgactccga caggcagctg ctggcgcacc cgccagtctc
     2461 cgccgaaccc gctggcgcgg tttcgggctg tcgctacggt ccgatgcgtg aaaaccccgg
     2521 gcttgcagcg accgttccgg tcgcgccgcc tctgtcgcgc cgtggcctgc gctcccggca
     2581 tcccggcaaa aggccgccgg gatgtaaggc gcaacgccgt ttcgcaaacc gcgcttcacg
     2641 tcgttgcggc gggtccctgc ccgttgttct gtccggtgcc attcacctgt ttaagcccct
     2701 gtcgccgggg gcatagacag ccacaacctg ccgcactgac tgtccagggt aaatgaacag
     2761 cccacaaaat tttttaccta aaacccggta aaaaattttg cgttctgccc aggacaggtg
     2821 cgtcaggccg tggcagtgat gccccttgcg gcgaaggttc tcaaaaaggt gatggtgaat
     2881 accggaggga acaaaaggca ggtccccgca aaacgtcaaa accgcggctc ccgaaactca
     2941 aaagatgatg ggggctgaaa acatcagata caggaggaaa catgagcgca ttttctttta
     3001 acaccgctta tcagccaaca ggcgatcagc agaaagacgc aatcgcacaa atcgggatca
     3061 tgcagaatcg tgctgttcag gcaaatcttg cataccagag cagcctggat gcggaaacgc
     3121 tattgaaaca gttatggcaa atcaatgatg aactggggga actgttggat agcgtggaaa
     3181 atcacacgcc agttatcaga aaaaaagcca gtgatttgag tgcactgatg atgcttttca
     3241 gccaacaggc cggtcagcct atgaccatgt tgttgatttg ttaatgatca atcaggggca
     3301 ggaaattaag cgaaagatgc tctgccccga gtttgcagcg accggaacgg tc
//
LOCUS       DAZGXZ010000266         3295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000266 DAZGXZ010000000
VERSION     DAZGXZ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.268"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1239
                     /gene="espX1"
                     /locus_tag="ACHKJF_005329"
     CDS             <1..1239
                     /gene="espX1"
                     /locus_tag="ACHKJF_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFP0952505.1"
                     /translation="LHHFFQYTHLRDAYFYRCKLIEHTVQFYFFKHKGITLLRLDVFD
                     DRTSECLSEEIKIYQECHEKFVNFLKANFNQEIYPQLYTPEIFYEACRNLQSFYDHQE
                     TSQNAKYSAIDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQNFK
                     TSNIAYSQLCKKLSLFKSIPSRLIKKSAYCSTENTITNKFDVVFSYCGNNVKEFILLL
                     PYNKSLEMHELNEQNIQYLTALNINIHKMLLSNITIEKSDLSYGYYFGCVLSNILCFE
                     SDLSNTIFSNGEINNLFIKKSNIFGASFTNTRIKNLLCEDIMPGRWTTQLVNKHLGYR
                     YTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSEMR
                     KYLKSINECMFLMKNIAHQN"
     gene            1486..2817
                     /locus_tag="ACHKJF_005330"
                     /pseudo
     CDS             1486..2817
                     /locus_tag="ACHKJF_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2830..3295
                     /locus_tag="ACHKJF_005331"
                     /pseudo
     CDS             2830..3295
                     /locus_tag="ACHKJF_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT         1166 a          605 c          546 g          978 t
ORIGIN      
        1 cttcatcatt tttttcaata tactcatctg agagatgcat atttttatag atgtaaactc
       61 atagaacata cagttcaatt ttattttttt aaacacaaag gtatcacatt acttagattg
      121 gatgtttttg atgacagaac aagcgaatgt ctttctgaag agattaagat ataccaagaa
      181 tgccatgaaa aattcgttaa ttttttaaag gcaaacttca accaagaaat atacccacag
      241 ctatatactc ctgaaatatt ttatgaggcc tgcagaaatc tccagtcatt ttatgatcat
      301 caggaaacgt cacaaaacgc aaaatacagt gcaatagaca agaaaaaaag tcatcttaac
      361 caggaaatca aaaagcttat taaaaaaaat attttccctg agctatacaa tgagcaatgc
      421 aataaaatcc cctcctcatc gatagatgat aaccagggaa taatgtggca gaactttaaa
      481 actagcaata ttgcatattc acaactatgt aaaaaattat ctttatttaa aagcataccc
      541 agtcgattaa taaaaaaatc agcttactgc tcaactgaaa acacaatcac caataaattt
      601 gatgttgtct tttcttactg cggaaataat gtaaaggaat ttatactttt attaccctat
      661 aataaatctc tggagatgca tgaacttaac gagcaaaaca tccagtattt gactgccctt
      721 aatattaata tacataaaat gttgctatca aatataacaa tagaaaagag cgacctttca
      781 tatgggtatt attttggttg tgtattatcc aatatattat gttttgaaag cgacttatca
      841 aacaccatct tttcaaatgg agaaattaat aatctcttca ttaaaaaaag caacatattt
      901 ggagcatcat tcactaatac cagaataaaa aatttgctct gtgaagatat tatgcctggt
      961 cgatggacca ctcaacttgt caataagcac ttaggttaca gatacacagg agtattcaaa
     1021 accctggcaa gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc attggtacaa
     1081 actttagttc gcgacaatgt aaagctaaat aatgatgtgt ataaagaact taataagttt
     1141 atgcacgatt acgacaaaac atcatcagaa atgagaaaat accttaaaag catcaacgag
     1201 tgtatgttcc ttatgaaaaa catagctcat caaaattaaa aaatctcaaa aaagaagtaa
     1261 tttcagaatg aaattaactg tgatgattat taattcacta cagaggaata aaaaataatc
     1321 tgaagcatac tctgaaacat cctactctct cactctaata atggcacctt gctcttctta
     1381 taaagaaggg aataattaag aatattaaac tttcatcaac ttcacatcaa gaaattaaaa
     1441 attttaaaca ttaatctaaa taataacaac ctgatcacat aatcgggtac gattttatcc
     1501 gataactata atgctataac tctgggaaat ggttggaata caccgctggg tgccatctct
     1561 ttttacgctg cccgatcgag tagtaagtta aataatgata ccagacacga aggtacgagc
     1621 tatcaggtcg cctataataa atatctagta caaacagcca ctcgatttag cgtggccgcc
     1681 tggcgttatg cttcgcagga ttacaggaca ttcagcgacc atctttacga aaatgataaa
     1741 cactatcatc agagtgatta tgatgatttt tatgatattg gcagaaaaaa tagcctttct
     1801 gctaatatta tgcaaccttt atccaataat ctgggaaatg tatcattaag tgcactttgg
     1861 cggaattact gggggcgaag tggaaatgct aaagattacc aattcagtta ctccaataac
     1921 tggcaacaca ttagttatac tttctctgcc agccaatctt atgatgaaaa taataaagaa
     1981 gaggagcgtt ttaatctgtt tatctccatt cctttctact ggggggatga tattgccaaa
     2041 acacgtcacc aaattaactt atcgaattcg acctcatttt ccaaagatgg ctattcctcc
     2101 aacaatactg gaattactgg catagccggt gaacatgatc agttaaatta tggtatatat
     2161 gttaatcagc aacaacaaaa taatgatacc tcgcttggta cgaatttaag ctggagaact
     2221 cccatcgcca taatagatgg cagctatagt cattctaaaa acgcctggca aagtggtgga
     2281 agtattagta gtggattagt tgtctggccc ggtggtatta atatcactaa ccagttatcc
     2341 gatacatttg caattctgga tgcgcctggg ttagaaggtg cacatattaa tggacaaaaa
     2401 tacaatcgaa caaacagcaa aggccaggtt gtttacgacc tgataatacc tcatcgtgaa
     2461 aaccatctgg tacttgatat agcaaacagt gaaagtgaaa cagaattgca gggcaatcgt
     2521 caaattattg caccttaccg tggagcagtt tcttatgtgc agtttacaac tgaccaacgt
     2581 aagccctggt atatacaggc actgcgtccc gatggttcgc cattaacctt tggctatgac
     2641 gtactggatc tccaggaaaa taatattgga gtcgttggcc agggtagtcg cctttttatt
     2701 cgcgtagatg aaattcccac tggaataaaa gttgctctca atgatgaaca gaacttattc
     2761 tgtactatta cttttcaaca cgttatcgat gaaaacaaaa catatatatg ccagtaaaag
     2821 gagtttacta tgaagtggct tttattaatc accttaagtc tatattcttt tattgtgcaa
     2881 agtgctccct gtgcattgac caatgtcggt gaacaacgtg gaacatatat actcaagcca
     2941 ctgagtatga aaggtaatct aaccgccgaa aaattgaaaa caccggaaat atcattgagt
     3001 gtacagactt gcaagagaca accaacgcca ttaaagttca ttttttaaca cagaacgctt
     3061 taaggtttaa agtcggaagt cgtcattata ttattaattt cccctttgaa tctgacggta
     3121 aaaaaataga gcttccaggg aagtcatctt ataatttaga agaaatgcta tctgcgataa
     3181 atttcaccat tccatattct atcgcatctg tatcagatag ttctaacacc gcagacattt
     3241 ttcctggaac tccttttcct ttgttgttcg aaattgaagt gagttcatgt acagg
//
LOCUS       DAZGXZ010000267         3282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000267 DAZGXZ010000000
VERSION     DAZGXZ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.269"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHKJF_005332"
     CDS             complement(<1..191)
                     /locus_tag="ACHKJF_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952506.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEHDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYS"
     gene            436..1908
                     /gene="cta"
                     /locus_tag="ACHKJF_005333"
     CDS             436..1908
                     /gene="cta"
                     /locus_tag="ACHKJF_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin 10"
                     /protein_id="HFP0952507.1"
                     /translation="MDKVTDNSPDVESTESTEGSFPTVGVDTGDTITATLATGTENVG
                     GGGGAFGGASESSAAIHATAKWSTAQLKKHQAEQAARAAAAEAALAKAKSQRDALTQR
                     LKDIVNDALRANAARSPSVTDLAHANNMAMQAEAERLRLAKAEQKAREEAEAAEKALR
                     EAERQRDEIARQQAETAHLLAMAEAAEAEKNRQDSLDEEHRAVEVAEKKLAEAKAELA
                     KAESDVQSKQAIVSRVAGELENAQKSVDVKVTGFPGWRDVQKKLERQLQDKKNEYSSV
                     TNALNSAVSIRDAKKTEVQNAEIKLKEAKDALEKSQVKDSVDTMVGFYQYITEQYGEK
                     YSRIAQDLAEKAKGSKFNSVDEALAAFEKYKNVLDKKFSKVDRDDIFNALESITYDEW
                     AKHLEKISRALKVTGYLSFGHDVWDGTLKGLKTGDWKPLFVTLEKSAVDFGVAKIVAL
                     MFSFIVGAPLGFWGIAIITGIVSSYIGDDELNKLNELLGI"
     gene            complement(1905..2195)
                     /gene="cti"
                     /locus_tag="ACHKJF_005334"
     CDS             complement(1905..2195)
                     /gene="cti"
                     /locus_tag="ACHKJF_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159421186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 immunity protein"
                     /protein_id="HFP0952508.1"
                     /translation="MTVKYYLHNLLESLIPWLFYLLLNYKTPPFSLFIFIASIHVLLY
                     PYSKLTIFSFIQNTTKMKKEPWYSYNLFYFLYLAMAIPVGLPSFIYYSLKRN"
     gene            2220..2351
                     /gene="ctl"
                     /locus_tag="ACHKJF_005335"
     CDS             2220..2351
                     /gene="ctl"
                     /locus_tag="ACHKJF_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin 10 lysis protein"
                     /protein_id="HFP0952509.1"
                     /translation="MKITFLIITILSLSGCQANYISDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(2566..2707)
                     /locus_tag="ACHKJF_005336"
                     /pseudo
     CDS             complement(2566..2707)
                     /locus_tag="ACHKJF_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            3081..3269
                     /locus_tag="ACHKJF_005337"
     CDS             3081..3269
                     /locus_tag="ACHKJF_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952510.1"
                     /translation="MDNGVTAFEKGESARRSRTTERSESVSEEAEEHRKLHFLLTHLC
                     GISHPAWCTFHTIRTDAT"
BASE COUNT          911 a          617 c          862 g          892 t
ORIGIN      
        1 gagtaagcct gggtgggatt cacaccatta ctgatttcac tgaccacatg acgctgatct
       61 gccagtgtca gcgtgtaaac ctgtttctgg atgaggtcac gggctttgtc atgctcatat
      121 ccggcagcaa caagggtgtc gagaagtcgt ttgtctgttt tgttcagttt atggcggggt
      181 gatttgctca catcgttctc cgttttttca tgatccaaca gttttatatc tcatcctgat
      241 ttgctccggt gttctgtttc gacgttctca atgcctgcct ccccatagtg tgatccttat
      301 tgcaaaaatg aaaatccatg ctcttgacat ggacaatgct gagtagtagg tttttactgt
      361 acataaaacc agtggttata tgtacagtat gttgttttta atttattgtt ttaattatca
      421 aagaggaatt tctttatgga taaagtcact gataattctc cagatgtgga gagcacagaa
      481 tctactgagg ggtcattccc aactgttggg gttgatactg gcgatacgat tacagcgacg
      541 cttgcaactg gaactgaaaa tgttggtgga ggcggtggag catttggtgg ggccagtgaa
      601 agttctgctg cgatacatgc aaccgctaaa tggtctaccg cgcagttgaa aaaacatcag
      661 gctgaacagg ctgcccgtgc tgctgcggct gaggcagcat tggcaaaagc gaaatctcag
      721 cgtgatgccc tgactcaacg tctcaaggat attgttaatg acgctttacg tgctaatgcc
      781 gcccgtagtc catcagtaac tgaccttgct catgccaata atatggcaat gcaggcagag
      841 gctgagcgtt tgcgccttgc gaaggcagag caaaaagccc gtgaagaagc tgaagcagca
      901 gaaaaagcgc tccgggaagc agaacgccaa cgtgatgaga ttgcccgcca acaggctgaa
      961 accgcgcatt tgttagcaat ggcggaggca gcagaggctg agaaaaatcg acaggattct
     1021 cttgatgaag agcatcgggc tgtggaagtg gcagagaaga agctggctga ggctaaagct
     1081 gaactggcga aggccgaaag cgatgtacag agtaagcaag cgattgtttc cagagttgca
     1141 ggggagcttg aaaacgctca aaaaagtgtt gatgtgaagg ttaccggatt tcctggatgg
     1201 cgtgatgttc agaaaaaact ggagagacaa ttgcaggata agaagaatga atattcgtca
     1261 gtgacgaatg ctcttaattc tgctgttagc attagagatg ctaaaaaaac agaagttcag
     1321 aatgctgaga taaaattaaa agaagctaag gatgctcttg agaagagtca ggtaaaagac
     1381 tctgttgata ctatggttgg gttttatcaa tatataaccg aacaatatgg ggaaaaatat
     1441 tccagaatag ctcaggattt agctgaaaag gcgaagggta gtaaatttaa tagtgttgat
     1501 gaagcacttg ctgcatttga aaagtataaa aatgtactgg ataagaaatt cagtaaggtt
     1561 gatagggatg atatttttaa tgctttagag tctattactt atgatgagtg ggccaagcat
     1621 ctagaaaaga tctctagagc tcttaaggtt actggatatt tgtctttcgg gcatgatgta
     1681 tgggatggta ccctaaaggg attaaaaaca ggagactgga agcctttatt tgtcactctg
     1741 gagaagagcg cggtagattt cggcgtggca aaaattgtgg cattaatgtt tagttttatt
     1801 gttggtgcgc ctcttggctt ctggggaatt gcaattatca caggtattgt ttcttcttac
     1861 ataggggatg atgagttgaa caagcttaat gaattgctag gtatttaatt tctctttaga
     1921 gagtaatata tgaaacttgg caatcctact gggattgcca ttgctaggta aagaaaataa
     1981 aaaaggttgt atgagtacca aggttctttt ttcatttttg tcgtgttttg tataaaagaa
     2041 aatatagtta gttttgaata tgggtatagt aatacatgta tagatgcgat gaatatgaaa
     2101 agtgaaaatg ggggcgtctt atagtttaat aacaaataga atagccatgg gataagtgac
     2161 tctagtaaat tgtgtaggta gtattttacg gtcattattt atgttgttat aggggtagaa
     2221 tgaaaataac gtttttgata attacaatat tatccttgtc gggatgtcaa gcaaattata
     2281 ttagtgatgt acaaggaggg acggtcgctc catcttcctc ttctgaactg acagggatcg
     2341 cggttcagta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2401 ctgcttgcaa acaaaaaaac caccgctacc aacggtggtt tgtttgccgg atcaagatct
     2461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccca aaacttgtcc
     2521 ttctagtgta gtcaaaatca ggctgatact tcaagaactc tgttactaac ttataaatct
     2581 cgctctgcta acctggttac caatggctgc tgccagtggc gttaagtcgt gtcctttccg
     2641 ggttggaccc aagagaacag ttaccggaga aggcgcagca gtcgggctga acggggggtt
     2701 cctgcataca gcccggcccg gagcgaactg cctaagtcgc cccgagatac cagaagacgt
     2761 gagctatgag aaagcgccac gtgtcccgca agggagaaag gcggaaaagg tatccggtaa
     2821 tcgatatggc gggaacgtgg gggcgcagga gggagcgatt caccggaaac ggtgatgatc
     2881 tttaagccac gtcgggtttc gccaccgctg gcggatttca tggttgagcc gtaaggctcc
     2941 cacagatgca ccgaaaaagt gtctgttttg tgaagtccgt caggggggcg gagcctatgg
     3001 aaaaagcgcc tgtgccgcgg cctttcccgg ggtcttcgtt ttttgcgcac atgttctttc
     3061 ccgggttatc cccggattct gtggataacg gggttaccgc ctttgagaaa ggggagtccg
     3121 ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg gaagagcacc
     3181 ggaagctgca ttttctcctt acgcatctat gcggcatttc acacccggca tggtgcactt
     3241 ttcatacaat ccgcactgat gccacatgat taagccagta ta
//
LOCUS       DAZGXZ010000268         3276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000268 DAZGXZ010000000
VERSION     DAZGXZ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.270"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..214
                     /locus_tag="ACHKJF_005338"
     CDS             <1..214
                     /locus_tag="ACHKJF_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952511.1"
                     /translation="QANAEPEEQVDPRKAAVEAAIARAKVRKLEQQQTNAEPEEQVDP
                     RKAAVAAAIARAQAKKAAQQKVVNED"
     gene            215..1273
                     /gene="rsxD"
                     /locus_tag="ACHKJF_005339"
     CDS             215..1273
                     /gene="rsxD"
                     /locus_tag="ACHKJF_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP0952512.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1277..1897
                     /gene="rsxG"
                     /locus_tag="ACHKJF_005340"
     CDS             1277..1897
                     /gene="rsxG"
                     /locus_tag="ACHKJF_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP0952513.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAQELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1901..2596
                     /gene="rsxE"
                     /locus_tag="ACHKJF_005341"
     CDS             1901..2596
                     /gene="rsxE"
                     /locus_tag="ACHKJF_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP0952514.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            2596..3231
                     /gene="nth"
                     /locus_tag="ACHKJF_005342"
     CDS             2596..3231
                     /gene="nth"
                     /locus_tag="ACHKJF_005342"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP0952515.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQYNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          769 a          835 c          876 g          796 t
ORIGIN      
        1 acaggctaat gcggaaccag aagaacaggt tgatccgcgc aaagccgccg tcgaagccgc
       61 tattgcccgt gccaaagtgc gcaagctgga acagcaacag actaacgcag aaccagaaga
      121 acaggttgat ccgcgcaaag cggcagttgc cgcggctatt gcccgcgctc aggccaaaaa
      181 agccgcccag cagaaggttg taaacgagga ctaaatggta ttcagaatag ctagctcccc
      241 ttatacccat aaccagcgcc agacatcgcg cattatgctg ttggtgttgc tcgcagccgt
      301 gccaggaatc gcagcgcaac tgtggttttt tggttggggt actctcgttc agatcctgtt
      361 ggcatcggtt agtgctctgt tagccgaagc tctcgtactc aaactacgca agcagtcggt
      421 agccgcaacg ttgaaagata actcagcatt gctgacaggc ttattgctgg cggtaagtat
      481 tccccccctc gcgccatggt ggatggtcgt gctgggtacg gtgtttgcgg tgatcatcgc
      541 taaacagttg tatggcggtc tggggcaaaa cccgtttaat ccggcaatga ttggttatgt
      601 ggtcttactg atctccttcc cggtgcagat gaccagctgg ttaccgccac atgaaattgc
      661 ggtcaacatc cctggtttta tcgacgccat acaggttatt ttcagcggac ataccgccag
      721 tggtggtgat atgaacacac tacgcttagg tattgatggc attagtcagg cgacaccgct
      781 ggatacattt aaaacctctg tccgtgccgg tcattcggtt gaacagatta tgcaatatcc
      841 gatctacagc ggtattctgg cgggcgctgg ttggcaatgg gtaaatctcg cctggctggc
      901 tggcggcctg tggttgctat ggcagaaagc gattcgctgg catattcccc tcagcttctt
      961 agtaacgctg gcgttatgcg caacgttggg ctggttgttc tcaccagaaa cactggcagc
     1021 accgcaaatt catctgctgt ctggtgcgac catgctcggc gcattcttta ttttgactga
     1081 cccggttacc gcttctacga ccaatcgtgg tcgtcttatt ttcggcgcgc tggcgggctt
     1141 attagtctgg ttgatccgca gtttcggcgg ctatcctgac ggcgtggctt ttgccgtcct
     1201 gctggcgaac atcacggttc ctctgatcga ttactacacg cgtccgcgcg tctacggcca
     1261 tcgcaaaggg taaaccatgc tgaaaactat ccgaaaacac ggcattacgt tggcgctatt
     1321 tgcagcgggt tcaacagggt taactgcggc catcaaccag atgaccaaaa cgacgattgc
     1381 tgaacaggcc agtctgcaac aaaaggcgtt atttgatcag gtgctgccag ccgaacgcta
     1441 taacaatgcg ctggcacaga gttgctatct ggtaactgcg caagagttag gtaaaggtga
     1501 gcatcgggtt tacatcgcca aacaggatga caaaccggta gccgccgttc tggaagcaac
     1561 cgcgccagat ggctattccg gtgcgattca gctgctggtg ggagccgatt ttaacggcac
     1621 ggtacttggc acgcgcgtga cagagcacca cgaaacgcca gggcttggcg ataaaatcga
     1681 actgcgcctt tctgactgga tcacccattt tgcgggtaaa aaaatcagtg gtgcagatga
     1741 tgcgcactgg gcggtgaaga aagatggtgg tgatttcgac cagttcaccg gcgcgacgat
     1801 tactccccgc gcggtggtta atgcggtaaa acgcgccgga ttgtacgctc agacgttacc
     1861 ggcacaactt tctcaacttc ctgcctgtgg agaataaagc gtgagcgaaa ttaaagacgt
     1921 tattgttcag gggttgtgga aaaacaactc tgcgctggtc cagctgctcg gcctttgtcc
     1981 tctgttggcg gtcacgtcca ctgccactaa cgctctgggt ttaggacttg cgactacgct
     2041 ggtactgacg ctgaccaacc tgaccatttc gacgctgcgt cactggacgc cagccgagat
     2101 ccgcattccc atttacgtga tgatcatcgc ctcggtggtc agcgctgtac agatgctgat
     2161 caacgcctac gcctttggcc tgtatcaatc attagggatt tttattccgc tgattgtcac
     2221 taactgtatc gttgtgggcc gcgctgaagc cttcgccgcc aaaaaaggtc cggcgctttc
     2281 ggcactggac ggcttttcaa ttggtatggg cgcaacctgc gccatgttcg tgctgggttc
     2341 actacgcgaa attatcggca atggcacatt gtttgacggt gcagatgcgc tgttaggtag
     2401 ctgggcaaaa gtattacgcg tggaaatttt ccacaccgac tcccctttcc tgctggcgat
     2461 gctgcctcca ggtgcattta ttggcctggg actgatgctg gcaggaaaat acctgattga
     2521 tgaaagaatg aaaaagcgcc gtactgaagc agctgccgaa cgtgcattgc caaacggtga
     2581 aacagggaat gtctgatgaa caaagcaaaa cgcctggaga tcctcactcg cctgcgtgag
     2641 aacaatcctc atcccaccac cgagcttaat ttcagttcgc cttttgaatt gctgattgcc
     2701 gtactgcttt ccgctcaggc gaccgatgtc agtgttaata aggcgacggc gaaactttac
     2761 ccggtggcga atacgcctgc agcgatgctt gaactgggcg ttgaaggggt taaaacctat
     2821 atcaaaacga ttgggcttta taacagcaaa gcagaaaata tcatcaaaac ctgccgtatc
     2881 ttactggagc agtataatgg cgaggttccg gaagatcgtg ctgcgcttga agccctgccc
     2941 ggcgtaggtc gtaaaacagc caacgtcgta ttaaacactg cattcggctg gccgactatt
     3001 gctgtcgaca cgcacatttt ccgcgtttgt aatcgtactc aatttgcgcc ggggaaaaac
     3061 gtcgaacagg tagaagaaaa gctactgaaa gtggttccgg cagagtttaa agtcgactgc
     3121 caccattggt tgatcctgca cgggcgttat acctgcattg cccgcaagcc ccgctgtggc
     3181 tcttgtatta ttgaagatct ttgtgaatac aaagagaaag ttgacatctg aagaaaaggg
     3241 gtaacaccga ttaccccatt gataaccttt ctttat
//
LOCUS       DAZGXZ010000269         3228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000269 DAZGXZ010000000
VERSION     DAZGXZ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.271"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKJF_005343"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHKJF_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP0952516.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKJF_005344"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHKJF_005344"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP0952517.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHKJF_005345"
     CDS             1252..1521
                     /locus_tag="ACHKJF_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP0952518.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKJF_005346"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHKJF_005346"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP0952519.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHKJF_005347"
     CDS             2601..3227
                     /gene="dsbA"
                     /locus_tag="ACHKJF_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP0952520.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          815 a          777 c          788 g          848 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctgct tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt
     1021 agcattaacc acgacgtgag agagctgcgc cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcca gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agccacgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gatttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag cggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaattttc cgtggttaac cggggaagat tactggctgc gacagacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gcatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaatatc tgtccgagaa aaaataat
//
LOCUS       DAZGXZ010000270         3196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000270 DAZGXZ010000000
VERSION     DAZGXZ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.272"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..720
                     /locus_tag="ACHKJF_005348"
     CDS             <1..720
                     /locus_tag="ACHKJF_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0952521.1"
                     /translation="HVQVGADKKHELDGLDLFTGVTMTYTYSNAGSDTFSGKTKSVGA
                     GLYASALFDSGAHIDLIGKYVHHDNEYTASFAGLGTKDYSSHSWYAGAEVGYRYHVTE
                     DAWIEPQAELVYGAVSGKQFSWKDQGMSLSMKDKDYNPLIGRTGVDVGKSFSGKDWKV
                     TARAGLGYQFDLLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFE
                     KSAFGKYNVDNAVNASFRYSF"
     gene            1448..1573
                     /locus_tag="ACHKJF_005349"
                     /pseudo
     CDS             1448..1573
                     /locus_tag="ACHKJF_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1633..2139)
                     /gene="yciE"
                     /locus_tag="ACHKJF_005350"
     CDS             complement(1633..2139)
                     /gene="yciE"
                     /locus_tag="ACHKJF_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0952522.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2185..2685)
                     /gene="yciF"
                     /locus_tag="ACHKJF_005351"
     CDS             complement(2185..2685)
                     /gene="yciF"
                     /locus_tag="ACHKJF_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0952523.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2771..2950)
                     /gene="yciG"
                     /locus_tag="ACHKJF_005352"
     CDS             complement(2771..2950)
                     /gene="yciG"
                     /locus_tag="ACHKJF_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0952524.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT          848 a          666 c          725 g          957 t
ORIGIN      
        1 cacgttcagg tgggtgcgga taaaaaacat gaactggacg gtctggacct cttcaccggg
       61 gtgaccatga catataccta cagcaacgcc ggcagcgaca ccttcagtgg taaaacgaag
      121 tcagtgggtg caggtctgta cgcttcagcc ctgtttgatt ccggggccca tatcgacctg
      181 attggtaagt atgttcacca tgataatgag tatacggcgt cctttgccgg actcggcaca
      241 aaagactaca gctctcattc ctggtatgcc ggtgctgaag tgggttaccg ctatcatgtc
      301 acagaggatg cctggattga gccacaggcg gaactggtct atggtgctgt atccggtaaa
      361 cagttcagct ggaaggacca ggggatgagc ctgtccatga aggacaagga ctacaatccg
      421 ctgattggcc gtaccggtgt ggatgtgggt aaatcgttct ccggtaagga ctggaaagtg
      481 acggcacgtg ctggtcttgg ctatcagttt gacctgctgg cgaacggaga aacggtactg
      541 cgtgatgcat ccggagagaa gcgtattaag ggcgagaaag acggccgtat gctgatgtct
      601 gtgggtctga acgcagaggt ccgtgacaac atccgttttg gtctggagtt tgagaaatca
      661 gcctttggta agtacaacgt tgataatgct gtcaacgcca gcttccgtta ttcattctga
      721 cggttgttac tgaaggggat tatggcctga cggctccctg taaataaaga caggcacaga
      781 agattgtgag ttactcagtg ttccaccaaa aagtaaggtc gcaactctgt gcctgcttcg
      841 taagtgtgca gtgagggcca tatggttata actgaagcat ccgacgacgt ggtgtctacc
      901 caaaaagcga cctgtgaata ttaggctggt taagtgcgta ttttcatgga aggagatcac
      961 ccaataaccc cgatctcctt tatgttaata cgcattaagc gggtaaaaca ggaagattaa
     1021 gaaagggaga gtttttcgac tccccaacca agagaagcag cttataacta ccagatagtt
     1081 gttatgtcat acaagacgga taatgtcata aaaaatatgt cattgccgtg attatgatcg
     1141 cgttattgga taaagcagtt cagcggtttc cccggcttcc tgcagcgttt cctgtacaaa
     1201 ttcgtgcgcg gattctttat ttacaactct taaaatggtg agtccatcat tgctggtggt
     1261 tctgacaatc acgtccacat ggtcatatcc gttagttatg gaatgactat aaggggggat
     1321 aagcctctgc aaaacaaaaa aagactttaa caaaatcaat cagttaaaaa cacagaaaaa
     1381 cggtatgaaa aatcaccata aaagtttgtt tttcctttta taaatagctg tttatcacat
     1441 tttttataat ctaccagtgt ggtacatgga tatcgatacc accgccaatt ataagctagg
     1501 cggtgcacag caacacgata gcgtacgcct cgatccgtgg gtgtttatgt tctcagcagg
     1561 atatcgtttt taattccgca caaaaacgac cccgtaatat acggggtcaa taaggacatg
     1621 gtataaaggg tattatttct tcgcttctac gccatcagtt tcagaacgaa ttaaaaattt
     1681 ctcagttgtt tgcggaatat gctgaatcag ccagtcggcc atttgctttt cctcatttaa
     1741 aatcgcttcg atgatcggaa ttgagactgt atcaccggca ttttttgctg ctgctaatag
     1801 tgaggtgtaa caggcgattt caaattgctc gaagacatat ccgctaatag agcctttaac
     1861 tatttcatca gaagggaata taccaccgat tgactgccca agcgcagcca ttttactcat
     1921 ggaatcttta atgactgaac gtgaaatgtc attacgatca agaatagttt ccagttgaac
     1981 aatctggttt ttggtttcac taagatgttg ttcaatacga gcgcgtagtt caggataatt
     2041 atctatacga ctggccatgg attcaagcat agattcggct tgcttttcca ttgcgtgggc
     2101 gtcacgtaac cagtcatgat aatgttcaat acgattcatg aaaatgtcct ctttataaaa
     2161 tgactaaaag ttaaaattca tatttcaggc tttattttcg gctttcttat ttacgttatt
     2221 aagggccaga tcagtcagtt tgatgtcggt ggccttttct tcttccaggg tttctttcag
     2281 aagcttcgct gctttacggt aacctaattg ttcagccagc gtcgctaatg tcccgtaact
     2341 ggcaatctca taatgctcga ctttctgtgc tgcggcaatc agtgcggcat cacgcacttc
     2401 gtttttctcg gtactttcga tgacctcatt agcttcttca ataagacctt ccattgccac
     2461 acatttcatg cgcttaattt tcaggttcga ttcggactcc acaacttgat caatacgttc
     2521 aatctgtcca tgagtttcct cgaggtgcgc atgaaaagcc tgacttaatt tttcatttga
     2581 tgttgctctt gcgagttttg ccagtgcccg ggttaattgt ttttctgcgc tgtaggtatc
     2641 tgaaagcagg tgaataaata catcttcaat ggtcttcata ttcatatttt tctccagtga
     2701 aatcactgcg agccatacgg cccgcagatg caattatctt tatcagctga tattaataat
     2761 taacggtgaa tcaggattta cctgatttat taccaccgct ttgttgaccg ccttttttac
     2821 ccgcttcaga tgcgcgttgc ggatcatttt taaaattacc gccgctatgc tgaccgcctt
     2881 tacggcctgc gtcggatgcc ttctcacggt cttcggcgaa atttcctgaa ccaccacgat
     2941 gttcggccat gttatttctc ccgttgcgtt gcattgtttc attaatatga gtgttgtgtg
     3001 tcgacactca ttaaaattag tcgctaatga gaattagtca aattaagcgc aacgagaaga
     3061 tagagggaaa atatattttg aggaacattc tggatatatt aacaattacc tgaggaataa
     3121 gtgacttaga gaaaattatt gattttactg gtgttatgtt gcgggattaa tttgtttgat
     3181 tatattactg ttgggc
//
LOCUS       DAZGXZ010000271         3165 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000271 DAZGXZ010000000
VERSION     DAZGXZ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.273"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(42..1949)
                     /locus_tag="ACHKJF_005353"
                     /pseudo
     CDS             complement(42..1949)
                     /locus_tag="ACHKJF_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(1969..2430)
                     /locus_tag="ACHKJF_005354"
     CDS             complement(1969..2430)
                     /locus_tag="ACHKJF_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP0952525.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(2458..>3165)
                     /locus_tag="ACHKJF_005355"
     CDS             complement(2458..>3165)
                     /locus_tag="ACHKJF_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0952526.1"
                     /translation="PADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQ
                     PIIMGRTYHQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKN
                     MDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQ
                     DRGTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVI
                     RLSKDGITIVGGSVFIN"
BASE COUNT          593 a          944 c          849 g          779 t
ORIGIN      
        1 gaattattta gtacaaaaaa ccggcgttac atgatctttc cctggctcca cgtctggtcg
       61 ttatcggacc agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc
      121 agccgcgcat ccgcttctgt cagctgcccc cgcgcatcct gttcgtaggt cgccagcgtc
      181 tgtatgccgt tatccgtacg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt
      241 ttaagccgga tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca
      301 aacgtgatgc ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg
      361 gggctgtgtg ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt
      421 cccggaaagg cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga
      481 taatcatcgc ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgt cgtatcactg
      541 tcaccgtcga aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc
      601 tgttcacgca cccaggcgta ttcgctccag gagtcgctcc agcccacgcc cgtcaggcca
      661 tgcagcggca gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagc
      721 gtaaagtcgg tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc
      781 catctgatga ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg
      841 ccgagctttg cgcccagcag cttcccgctt ttccccagca ggtttcccag tttgccgagt
      901 ctcgccagat tcttcagttt tcccagcagt cccgcaggcg gcagaagata gctggctatc
      961 gttattcccc ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatag
     1021 gtcacctgct caccgccaat aaagacgtcc ggcgaacctc ccacgatcac cgcactgcac
     1081 tccagtttgt cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc
     1141 tgggctatcc tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc
     1201 gggaaatcca cctctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt
     1261 aataccggac cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg
     1321 gcattgctgt ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct
     1381 ttttccgctg ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt
     1441 gggacttcag gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg
     1501 gccagctcgc cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca
     1561 caaaagctgt ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc
     1621 ttatagctgg catacatcag cgcaggcacc agaaccagcg cataagggcc ggtggcacag
     1681 gcggccataa tcaggcaacc ttctgcgatg gtcactatcg cccccagcac ggcccctgcc
     1741 agcgccccca aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggccg
     1801 gacttttttg atgccagcat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg
     1861 ctggcgacca tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct
     1921 ccctgtggcc cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg
     1981 gtccttttgg atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag
     2041 catcgtcttt tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt
     2101 atctccacgc cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc
     2161 cggaccgttc caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg
     2221 gcaggcatgt tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta
     2281 cagctctggt gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca ggttgatgcc
     2341 actgttatct gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg
     2401 aatgtggcct tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca
     2461 gttgatgaaa acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt
     2521 cgattccccg acctgtagct gaatgccatc cggcgtcagc gcaattttgc tttcctccac
     2581 ggccagcaaa atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt
     2641 gtttacagcg tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc
     2701 agataaagac cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg
     2761 gttaccttcc tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt
     2821 tacggtggtg gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta
     2881 gacctgttct ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta
     2941 cgttttggag cggatggtca tctgcgtctt tgttcccggc aggctgccgg gggggcggtt
     3001 ttcctggtgg taggtgcgcc ccataatgat cggctggtcc ggatcgccgt tgaggaagtc
     3061 tacaatcacc tcctgaccca cacgcggtat cgccaggtgg ccaaaaccgg tgcctgccca
     3121 cgcctgtgcc acacggatcc agcacgaact gtcttggtct gccgg
//
LOCUS       DAZGXZ010000272         3105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000272 DAZGXZ010000000
VERSION     DAZGXZ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.274"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(284..433)
                     /locus_tag="ACHKJF_005356"
     CDS             complement(284..433)
                     /locus_tag="ACHKJF_005356"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0952527.1"
                     /translation="MEQKTLSAEPRRSFSNNFKLQMVKLALQLGASVARIAQEHDIND
                     NLLFK"
     gene            497..775
                     /locus_tag="ACHKJF_005357"
                     /pseudo
     CDS             497..775
                     /locus_tag="ACHKJF_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(976..2204)
                     /locus_tag="ACHKJF_005358"
     CDS             complement(join(976..1889,1889..2204))
                     /locus_tag="ACHKJF_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0952528.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEQLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQQASNGLSDNRCLEI"
     gene            2487..2747
                     /locus_tag="ACHKJF_005359"
                     /pseudo
     CDS             2487..2747
                     /locus_tag="ACHKJF_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2729..2815
                     /locus_tag="ACHKJF_005360"
                     /pseudo
     CDS             2729..2815
                     /locus_tag="ACHKJF_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2812..>3105
                     /locus_tag="ACHKJF_005361"
     CDS             2812..>3105
                     /locus_tag="ACHKJF_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952529.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRN"
BASE COUNT          766 a          770 c          761 g          808 t
ORIGIN      
        1 tccatttttc tttcttccag tcaggactgc atctgattaa tcgtaagcgc tccgggaagt
       61 acgtcgcgta aggattattt tacagctgag aagtttcagt gcagcagttc atgcacacgg
      121 ttcgacggct agtcattgat cttcataatt acctcgcgta aaaaacgggg taaggccgct
      181 catccagcgt catctccaca ggtaataatt caactccagt gtcagaagag gttgttaccg
      241 aaagacgccg agatatacgc ccttcgtttt gccagagtct gagctatttg aacaggaggt
      301 tatcattgat atcgtgttcc tgggcgatac gggcaacaga ggctcctagt tgcaaagcca
      361 gtttaaccat ttgaagttta aaattatttg aaaatgatct gcggggttct gcggataatg
      421 ttttttgctc cataacaggc gtccactagt ttgaaaaggt gggcacctac gttaccaata
      481 ctggcttaat ggctacatac ggcggtcggt ttacgcttac gaaatacggg gattaccgga
      541 gtcagagcgt ctttccgtca ggcagcagcg aagcaaagtg ttactgacgt cgctgcatga
      601 atggatggtg gagaagaatg gtacgctgtc gaaaaaatcc agactgggcg aagcgttcag
      661 ctatgtactg aatcagtggg atgccctctg ttattacagt gatgacggtc tggcggaggc
      721 ggataataat gctgcggaaa gagcgcttcg tgcagtctgt ctcggaaaga aaaactctta
      781 tgacctttgt caaatattga gcccaaaaag accttacgca gctccgggag cttcgttata
      841 tcgcggatta aagaacctga aacagtcaga ctgtatcttg gattttacaa actcttattc
      901 ggggatttta gccccatgac gatttacgca gtgagctggc ctctttctat atgcaggtat
      961 tggctggatt tgcccctata tttccagaca cctgttatca cttaacccat tactggcttg
     1021 ctgccgtaga tattcccgtg gcgagcgata acccagtgca ctatgcggat gccattcgtt
     1081 ataatgctcg aacgcctctg caaggttctt tgctgccgtt aacccgtctg gtttgggcat
     1141 gacactgatg tagtcacgct ttatcgtttt cacgaagctc tctgctatgc cgttactctc
     1201 cggactccgc accgccgtgc tcttcggttc aagccccaac atccgggcaa actgccgtgt
     1261 ttcattagcc cggtagcatg aaccattatc cgtcagccac tctactggag acgccggaag
     1321 ctcgttgccg aagcggcgtt ccaccgctcc cagcatgacg tcctgtactg tttcactgtt
     1381 gaagccgccc gtagtgaccg cccagtgcag tgcctcacgg tcacagcagt ccagcgcgaa
     1441 cgtgactcgc agttgttctc cgttatcaca gcggaactcg aacccgtcag agcaccatcg
     1501 ctgattactt tctttcacag ccactctgcc tgtatgtgcc cgtttcgatg gcggtacagc
     1561 aggttttcgc tcaagcaaca gcgcattctg gcgcatgatc cggtaaacac gtttggcatt
     1621 gatcgcaggc ataccatcaa gttctgcctg tctgcgaagc agcgcccata cccgacgata
     1681 accatacgtg ggcagctctc cgataacatg gtgtatacgg agaagcacat ccgtatcatc
     1741 agtgtgacga ctgcggcggc catccatcca gtcatcggtt cgtctgagaa tgacgtgcaa
     1801 ctgcgcacgc gacacccgga gacaacggct gactaagctt actccccatc cccgggcaat
     1861 aagggcgcgt gcgctatcca cttttttgcc cgtccatatt caacggcttc tttgaggagt
     1921 tcattttcca tcgttttctt gccgagcagg cgctggagtt ctttaatctg cttcatggcg
     1981 gcagcaagtt cagaggcagg aacaacctgt tctccggcgg cgacagcagt aagacttcct
     2041 tcctggtatt gcttacgcca gagaaataac tggctggctg ctacaccatg ttgccgggca
     2101 acgagggaga ccgtcatccc cggttcaaag ctctgctgaa caattgcgat cttttcctgt
     2161 gtggtacgcc gtctgcgttt ctccggccct aagacatcaa tcatctgttc tccaatgact
     2221 agtctaaaaa ctagtattaa gactatcact tatttaagtg atactggttg tctggagatt
     2281 cagggggcca gtctattggc gcattctgac ctcctgaatt cttttacgag gatcactcat
     2341 tccgtgtctg actgcttcag ggtttaattt tgcttctgct gactgaatga tgattgggca
     2401 gcaagtgcaa tatcccacat aatgaggtag cctgagttta acggacactc cttcctgaaa
     2461 tagaatggca tcagaaggag ctaataatga gcagaaaaac ccaacgttac tctaaagagt
     2521 tcaaagccga agctgtcaga acggttcttg aaaatcaact ttcgatcagt gaaggcgctt
     2581 cccgattatc tcttcctgaa ggcactttag gacaatgggt taccgccgcc agaaaagggc
     2641 tcggaactcc tagttcccgc acggtggctg aactggaatc tgaaattctg caactgcgta
     2701 aggcgttaaa tgaagctcgc cttgagcgag atatattaaa aaaagcaact gtagattcaa
     2761 ttggtcaacg caacagttat gtgaaaacat ggggttgcgg aggttttttg aatgagacga
     2821 acatttacag cagaggaaaa agcctctgtt tttgaactat ggaagaacgg aacaggcttc
     2881 agtgaaatag cgaatatcct gggttcaaaa cccggaacga tcttcactat gttaagggat
     2941 actggcggca taaaacccca tgagcgtaag cgggctgtag ctcacctgac actgtctgag
     3001 cgcgaggaga tacgagctgg tttgtcagcc aaaatgagca ttcgtgcgat agctactgcg
     3061 ctgaatcgca gtccttcgac gatctcacgt gaagttcagc gtaat
//
LOCUS       DAZGXZ010000273         3015 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000273 DAZGXZ010000000
VERSION     DAZGXZ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3015
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.275"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..198)
                     /locus_tag="ACHKJF_005362"
     CDS             complement(<1..198)
                     /locus_tag="ACHKJF_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0952530.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWH"
     gene            complement(198..>3015)
                     /locus_tag="ACHKJF_005363"
     CDS             complement(198..>3015)
                     /locus_tag="ACHKJF_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0952531.1"
                     /translation="YSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGA
                     MTEDDARPEALRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAAG
                     SARAASTSAGQAATSAQEASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSE
                     TNAAASQKSAATSASTATTKASEAATSARDAAASKEAAKSSETNASTSASSAASSATA
                     AGNSVKAAKTSETNARSSETAAEQSASAAADSETAAASSAREASIKAEEAAVSATAAG
                     KSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETSAESSKTAAASS
                     ASSAASSASSASASKDEATRQASAARGSATTASTKATEAAGSAAAAAQSKSTAESAAT
                     RAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYDLANG
                     KYTAQDATTAQKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGI
                     VQLSSATNSTSETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLN
                     STTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGN
                     PKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHT
                     SPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAY
                     PKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTS
                     SFDYGTKSTNNTGAHTHSLSGSTNAAGNHSHRDGRRFNPSVFKDTYQYGYTSSGQNTW
                     GVQGSVGMSTGWLANTSTDGNHSHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTI
                     TVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          578 a          828 c          796 g          813 t
ORIGIN      
        1 atgccacgat gccttatcac tgttgaaaac agccacaaag ccagcaggaa tatctggtgg
       61 tgcaatatcg gtactgtttg ctggcagacc tgtatgaggc ggaatatatg cgtcaccttc
      121 accaataaat tcattagttc cggccagcag attataaatt tttatggtcc gtggttgttc
      181 actcattctg aatgccatta tgcaagcctc acaatatagt taaatgcgat gtttttgacg
      241 gtgttttccg cgttaccagc agcgttaacg gtgatggtgt gtccatgtga accaatcgca
      301 acggagtgcg tatgagcacc aataccgaca gtatgcgcgt gtgcacctgc agatgctgct
      361 gtgccggaca gtgaatggct atgattacca tctgtactgg tattcgctaa ccaccccgta
      421 gacataccta ctgagccttg tacaccccag gtattttgac ctgagcttgt ataaccatat
      481 tgataagtat ctttaaaaac actggggtta aatcgacggc catctctatg gctgtgatta
      541 ccagctgcat tcgtgctgcc acttaaacta tgggtatgcg caccagtgtt attcgtggat
      601 ttagtgccgt aatcaaacga cgatgtggtt ttcgtcccca aatccgtact ggatgcgctg
      661 gcgctgtggg tatgcgattt aatgccgtcc tgttcctgag acaatacggc ccgaccactg
      721 gcaggtttgc ccttaatcgt ccagccacgc atatcaggga tcacgcctga cggataagcg
      781 gctgcaagtt tcgggtatgc agatttgtca aaagtctgcc cctgcatcag ggcatagcca
      841 gacggaacgg tatctgatgg ccacgggatt ggtgcgccag gcggataaaa ctgctctgat
      901 ggcgtataga gtgaataaac tgtaccgtcc gttaaccctt ccggcttatt agcagaatat
      961 gctggtgacg tatgaatcgt cacgctggca ttactggtat aatcccattg aatatttaca
     1021 ccagtcgcat aatttccgat tgcaacgtaa atatcgtaag tatcaccaga tgtattgacc
     1081 caggcaaaat ttgtaaaccc tgtcgatgtg cgctgccata aagcaccagt aatccccttc
     1141 ggattaccat tacctgcacg taaaacaagt tcagatatac ctgcctgttg aggtgacccc
     1201 acgttaaatc cagcgccacc aatcaacgta attgaaacaa cagaactcgc ctgtggcatg
     1261 gttaccgttg ctaatttgaa ccaaccagca ccaccgctga atgacattgt tgttgagtta
     1321 agcgtaccaa tatctttcgg cgtcagtgtt atatccgctg aaagcgcctt accattcacc
     1381 ttacgggcag aaggtacccg accattcgca ttgtcattag ctgctttcac tgctttcggt
     1441 gtcgcagcaa gcgtttcaga tgtgctgttg gttgcactac tgagctggac aattcctttt
     1501 tgtgctgtcg tagcgtcctg tgcggtgtat ttcccgttag ccaggtcata cgcggcctta
     1561 acggcttttg gcgttgccgc ctgtgactcg gaagtgctgt tagtcgcact gctgagctga
     1621 actatccctt tctgtgctgt cgttgcatcc tgtgcggtgt atttcccgtt agccaggtca
     1681 tacgcggcct taacggcttt tggcgttgcc gcctgtgact cggaagtgct gttggtcgca
     1741 ctgctgagct gtactatccc ctttttcgtc gtgctcgcat cctcaagcgc cacggcggat
     1801 gcaatatcct ctgcccgttt tgctgctgtc tcggcgcgcg ttgccgcgga ttccgccgta
     1861 cttttgctct gtgctgccgc tgccgcactg ccagcagcct ctgtcgcctt cgtggatgcc
     1921 gtcgtggcgc tgcccctcgc tgctgacgcc tgtctggtcg cctcatcttt tgaagcagac
     1981 gcagatgatg ccgatgacgc cgccgaactg gcggacgatg cggcagccgt ttttgaggat
     2041 tctgcgctgg tttccgacgc tttcgcgttc gtttcggatg tcttcgctgc ggaagcagac
     2101 ctcgctgctg cgctggcctg ttcagtggct tcgccagcct tcgttgtggc tgttgaagcg
     2161 gatgatgcgg cgctttctgc cgattttccg gcggcggtgg cactgactgc tgcctcctct
     2221 gccttaatgg acgcctcacg ggccgatgat gcagctgctg tctcagagtc tgccgcagct
     2281 gaggcgctct gttccgctgc cgtttcagaa gacctggcat tcgtctcgga cgtttttgcc
     2341 gccttcacgg aatttcctgc cgccgttgcc gaggaggctg cactgctggc gcttgtggag
     2401 gcgttcgttt ctgatgattt tgccgcctct ttggaggccg ccgcatctct ggctgaagtg
     2461 gcggcttctg acgctttcgt cgtcgcagtg gatgcagaag tggctgctga tttttgtgac
     2521 gctgcagcat tcgtttctga cgttttcgcc gcaccggcac tggtggccgc cgcgcttttt
     2581 gaggactctg cagcggcagc actttttgat gcttcagtgg cctttgttga tgccgttcct
     2641 gcgctggaag acgcctcctg agccgaagtc gcggcctgtc cggctgacgt gctggctgca
     2701 cgcgctgagc ctgcagcatc agtcgcatgg gttgccgcct cacgggcaga tgtgccggca
     2761 tcactggctg acttcttcgc ggctgccgtg ttctgtgcca ccgcggacgc gttacgcgcc
     2821 acctcttcca ccatcagttc aaaacggcgc agagcctccg gacgggcatc atcctccgtc
     2881 atggcaccga gaaaatcatt cagcgtaccg ggtcgggaat cttcatacac ggtgatggtc
     2941 ccggcatgtg acggcgggaa tccttccacc aacagaataa cgctgtactg accgtactca
     3001 acgtccatgc tgtaa
//
LOCUS       DAZGXZ010000274         2990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000274 DAZGXZ010000000
VERSION     DAZGXZ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.276"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            64..396
                     /gene="hdeA"
                     /locus_tag="ACHKJF_005364"
     CDS             64..396
                     /gene="hdeA"
                     /locus_tag="ACHKJF_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP0952532.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            512..838
                     /gene="hdeB"
                     /locus_tag="ACHKJF_005365"
     CDS             512..838
                     /gene="hdeB"
                     /locus_tag="ACHKJF_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP0952533.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            902..1549
                     /locus_tag="ACHKJF_005366"
     CDS             902..1549
                     /locus_tag="ACHKJF_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP0952534.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1591..2121)
                     /locus_tag="ACHKJF_005367"
     CDS             complement(1591..2121)
                     /locus_tag="ACHKJF_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0952535.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHKJF_005368"
     CDS             complement(2277..2843)
                     /gene="slp"
                     /locus_tag="ACHKJF_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP0952536.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          864 a          595 c          661 g          870 t
ORIGIN      
        1 tataacctca gtgtcgaaat tgattcgtga cggctctttc actttatagt tgaggatatt
       61 acgatgaaaa aagtattagg cgttattctt ggtggtctgc ttcttctgcc agttgtaagc
      121 aatgcagcgg atgcgcaaaa agcagctgat aacaaaaaac cggtcaactc ctggacctgt
      181 gaagatttcc tggctgtgga cgaatccttc cagccaactg cagttggttt tgctgaagcg
      241 ctgaacaaca aagataaacc agaagatgcg gttttagatg ttcagggtat tgcaaccgta
      301 accccagcta tcgttcaggc ttgtactcag gataaacaag ccaactttaa agataaagtt
      361 aaaggcgaat gggacaaaat taagaaagat atgtaattcc gggaatgcgt tacatcgtac
      421 ttccttgcat attgaacagg ccggaatatc ttctttaaaa gcagctattc ctcctgttca
      481 tatataatct ctatattgaa tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt
      541 tatttttatg ggcgctgtag cggctttgtc actggtgaac gcacaatctg cgctggcagc
      601 caatgaatcc gctaaagata tgacctgcca ggaatttatt gatctgaatc caaaagcaat
      661 gaccccggtt gcatggtgga tgctgcatga agaaacagta tataaaggtg gcgataccgt
      721 tactttaaat gaaaccgatc tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa
      781 cccgcagaaa aatttgtata ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg
      841 aggtacaagt aaaaaggagt agcaagttga gccatcttgc tgctcctttt tgcattttta
      901 tatgacagca gaatttatta tacgtcttat actcgcggca attgcctgtg gcgctattgg
      961 catggaaagg caaatgcgcg gcaaaggagc agggttacgc acacatgtat taattggcat
     1021 gggaagcgcc ctgtttatga ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga
     1081 tcacgtcgga ctcgacccca gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat
     1141 cggtgcaggt aacattctcg ttcgtaacca aaatattgta ggtctgacga cggcagcgga
     1201 tatctgggtg accgccgcca taggtatggt tattggcagc ggtatgtacg aactgggtat
     1261 ttatggttca gtgatgacct tgttggtgct ggaagtcttc catcaattaa ccttccgcct
     1321 gatgaacaaa aattaccatt tacagctgac attagtgaac ggtaatacgg tatcaatgct
     1381 cgactggttt aagcagcaaa aaataaaaac ggatttagtt tcgttgcagg aaaatgaaga
     1441 tcatgaagtg gtcgccattg atatccagct tcatgccaca acgtcgatag aagacctgtt
     1501 gcgattatta aaaggtatgg caggggtgaa aggcgtttca attagctaat ctgtgtggca
     1561 gcgtagccag actcaccgta agcctgaaat tcacaccaga taatcaatat gctgatggcg
     1621 taccagctca ttgatccgct taagattgag gatcatcatg atattgtgtt tatggcagta
     1681 gaacgttttt agcgatattt tcagtttctc cagaatatct tccgttgtca tttgattcgc
     1741 aatgcagctc agaacctgat gttgcttgcg cgaaagtgac acagccgctt caggcacaga
     1801 gcagtaactg ccattgatga gtgcgacaaa atcaatgata gtgatagtcc gcggaacaaa
     1861 ggtcaccggc actttcccca ggcagcgttt aatattaaag ggggagaaaa taatcacatg
     1921 gacaggtttt aatcgttcaa tttgagtgag aaaattagaa tgaaatacat tattcattaa
     1981 tgtatcaata atgacgaagg cattctgatg caacattacg tcgatctctt cttcgttctg
     2041 tatatgaacg acattacctt tactcagaat gtttttcatc gcggtgaaga acatcgtatc
     2101 cctggtaatt ataagaaaca taaccgactc ctggtgcgga cattaacttt ataatcagtt
     2161 tgccaaaggt aattaacagg acagggctta taataataat agttagctta ctgtatatta
     2221 attttatgta atacatgatg cagatgctga gaaagcatct gcatctttcg gtggtgttat
     2281 ttgaccagct caggtgttac ctgactcacc gcattggtgt agtaaggcgc accccagccc
     2341 ggttccggcc agaatgcacc atagccgtaa tcccacaggt tataggtggt attaaccact
     2401 tctctcaaat gccacacctg gatgccctgc atattcactt ccaggaagtt atacgggact
     2461 ttattgataa agcctggttg ttcaccctga atggtaccga ggatggtaac aaagtgatta
     2521 cgatagttca ctggatcaag gaagccgctt tgtctggcga gcagtcggcc ctgatagttg
     2581 gcttcaatat caggcttcgc atagctatcc aacggtaata cagcgatttc taacaacgta
     2641 tccgttttgc cattgataac gttgataacc ttcccaccaa agcgcgcttg ttgaccaaca
     2701 tataaccccg gctggttatg aacagcaaca aaactttttt gaatatcagg ttggttattg
     2761 cctttgatat tttgcggaat tgaactacat gcggcaagca aaaatgaaag gctgaggatg
     2821 agtgcacctt ttgtcatgtt catgttacta tccttatcaa caaatattaa aaccataata
     2881 gatatttctt tatagttttc atctgattct gaggtgatat gcctgatagt aacgtaaacc
     2941 tgtctgatgt ttatatccct tctgtttagt tcaattccgt tattttgggg
//
LOCUS       DAZGXZ010000275         2983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000275 DAZGXZ010000000
VERSION     DAZGXZ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.277"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..250
                     /locus_tag="ACHKJF_005369"
     CDS             <1..250
                     /locus_tag="ACHKJF_005369"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952537.1"
                     /translation="TYENGQVSNKHRHVYFFPDKSVKKGEYVILFTRSGKNKTGTWND
                     DDDTVTHRFYWGLDTTVWNEDGDEALVLKYRIVDRKKV"
     gene            403..960
                     /locus_tag="ACHKJF_005370"
     CDS             403..960
                     /locus_tag="ACHKJF_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0952538.1"
                     /translation="MAIFGYGRVSTAQQDTENQRLELEQAGWTFDFWFADIVSGKAQA
                     IQRKAFAEMLGKIRTGETLVVAKLDRLGRDAIDVLQTVRLLSDRGIKVIVHQLGTTDL
                     TSAAGKLLLSMLAAVAEMERDLLIERTQAGLSRAKAEGKKLGRPSKIAPEARRAIIEK
                     KSSGISVSALAREYGVSRATIAALL"
     gene            1483..>2983
                     /locus_tag="ACHKJF_005371"
     CDS             1483..>2983
                     /locus_tag="ACHKJF_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005185975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952539.1"
                     /translation="MASPSLEDSDYEELLELIKIENKILPSDKLESLPLNKDHIPVSA
                     VNDQVIKIKELSSLVHVNRIAPDQKIAFCDNGITIIYGMNGAGKSGYGRVLKHACRAR
                     DKGGAILPNVNLPGYSSNIPNGKFKISLNGKEHDLQWSYNSPSPELLSSISVFDTLCA
                     SSYLKEGEAAYLPRGLDILEGFANIIIPKLAEKVLAEAKAIDVNKEITQIFTDGTSVS
                     DICKVLDHKTDVVKLRDLATLSDSELSRDKELDRVLAEKNPEEKYKELLRSVSHIKEL
                     CVRINNQSKFINNAAMIKLKSLIEGVTNAEKALNESAQKLAAGDDLLPNTGEGIWRDL
                     FLSAKKYSLSCAYPGHDFPSVGRDAKCVLCQQPISEATDRMLRFNEFINDDISKLLDE
                     RKSSLKTAREKIYTATLDFSFNAAIHIEIGNEHSELIERVNKYQEDIINTKTAMLSMI
                     DSKNFANIEFDYNPISELRLLAASKLREARLYKQASKKRKIDALIKESKE"
BASE COUNT          935 a          513 c          683 g          852 t
ORIGIN      
        1 cacttatgaa aatggacaag tatctaataa acatcggcat gtctactttt tcccggacaa
       61 aagcgttaaa aaaggagagt atgttatttt gtttacacgc tcagggaaaa ataagactgg
      121 aacatggaat gatgatgatg acactgtgac tcatcgtttt tactggggct tggatactac
      181 agtctggaat gaggatggag atgaagcctt ggttctgaaa taccgaatag ttgataggaa
      241 gaaggtttaa tatccaccaa attgactttt gccggaaatc gaccgttttc cggcacccct
      301 tcgcaggtat cttccgggat gttgaaaaat tagccttggc aattgttggc aggcgtccta
      361 ttgttgacac cgttttctga caggtattga gaggtgttga gaatggcgat ttttggctac
      421 gggcgtgtct ctaccgctca gcaagatacg gagaaccaga ggttggagct tgagcaagct
      481 ggttggacgt ttgacttttg gtttgccgat attgttagcg ggaaagctca ggcaattcag
      541 cgtaaggcgt ttgctgaaat gcttggcaaa attcgaaccg gtgaaacgct ggtagtggca
      601 aaactcgacc gtttggggcg tgatgctatc gacgtgctcc agacagtccg attgttatct
      661 gatcgcggca tcaaggtgat cgtccaccag cttggaacaa ctgacctcac ttctgctgcc
      721 ggtaagcttc tattgtcgat gttggcagcg gttgcggaga tggagcgtga tctactgatt
      781 gaacgcactc aggcgggttt gtcgcgagca aaagccgaag gcaagaaatt gggtagacct
      841 tctaaaatcg caccagaagc ccgtagagcg attatagaga aaaagagtag cggaatcagt
      901 gtcagtgcgt tagcgcgtga atacggcgtt tccagagcaa ctatagccgc gttgctttaa
      961 acgtttattg cttctcgaag ctcttgggcg acaactcgtt gtatagcggt tctaactttc
     1021 agaccgcagg gcttatttca gagaaagatt gccaatgcac ttaatcattt gggcataact
     1081 acgcctcatg gtggaacttg gcgattgtct caggttcatg gaataatcag taggttagaa
     1141 gaaaatgtga aagaagagac tcagtttaaa atgctttcgg ttgcgttaaa tgaattcttc
     1201 acttaaaaca taacggtgtc gcatatgtcg atgtttatga gagagttaag ttaaattgaa
     1261 gagcgtgttc ttctttgttc caatgtcttc ggtggatgcg atacagtcta accttatgaa
     1321 ttgcttatga aatcttctat caacgctatt atatgaaaga taggaggcaa ttcttatcta
     1381 taaaaataac tgttagaggc tatcatggat gtcttacagg aaatattgaa atggtctagt
     1441 tccgagttac ccctctgggc aagagactgc ttaaggagac aattggcttc tccctcattg
     1501 gaggatagtg attatgaaga attattggaa ttgataaaga ttgaaaataa aatattgccc
     1561 tccgataaat tagaatctct ccccttaaat aaggatcata ttccggtttc agcggttaat
     1621 gatcaagtca ttaagattaa agaactaagt agtcttgttc acgtgaacag gattgcgcct
     1681 gaccaaaaaa ttgcattctg tgataatggg attaccatca tttatggtat gaatggtgcg
     1741 ggaaaatcag gatacgggcg agttctcaag catgcatgta gagcacgaga taaaggtggt
     1801 gcgatactcc ctaatgtaaa cctgcctggc tattcatcca atatacctaa cgggaaattt
     1861 aaaatttctc tgaatgggaa agaacacgat ttacaatggt cttataattc accatcccca
     1921 gaattgttgt cctcaattag tgtttttgat actctttgtg catcatcata tctgaaagaa
     1981 ggcgaggctg cttatttacc tcgcggtctc gatattcttg aaggatttgc aaatataata
     2041 attcctaaat tagcagagaa agttttagcc gaagctaagg ctatagatgt gaataaagag
     2101 ataactcaaa tttttacaga tggcactagt gtatctgata tatgtaaagt attggaccat
     2161 aaaacagatg tcgtaaaatt aagagattta gccactttaa gtgattccga gctaagtaga
     2221 gataaagagc tagatagagt tctggcagaa aaaaatcctg aggaaaagta taaagaatta
     2281 ctaagaagtg tatcgcacat aaaagagctt tgcgttagga tcaataatca aagtaagttt
     2341 ataaataacg cggccatgat aaagcttaag tcgttgatcg aaggcgttac taatgcagaa
     2401 aaggcattaa atgagtcagc gcaaaaactt gcggccggag atgatttatt gcctaacact
     2461 ggtgaaggta tctggagaga tttgtttctg tctgctaaaa aatactcgct cagttgtgct
     2521 tacccaggcc atgattttcc ctccgttggt cgtgatgcaa aatgcgtttt atgccagcaa
     2581 cctatatccg aagcaactga cagaatgtta cgcttcaatg agtttattaa tgatgatata
     2641 tctaaattat tagatgaaag aaaatcttct ctaaaaacag ctagagaaaa aatatatact
     2701 gctacccttg attttagttt taatgctgca atacatattg agattggcaa tgagcatagc
     2761 gaattaattg aaagagttaa taagtatcaa gaagatatta tcaatacaaa gactgcgatg
     2821 ctttcaatga ttgatagtaa gaacttcgcg aatatcgaat ttgattataa tccaatttca
     2881 gagctaagac ttctggctgc tagcaagtta cgtgaagcaa ggttatataa gcaagcatcc
     2941 aaaaaaagaa agattgacgc tctaataaag gaaagtaaag aac
//
LOCUS       DAZGXZ010000276         2930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000276 DAZGXZ010000000
VERSION     DAZGXZ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.278"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1264..1866
                     /gene="fimB"
                     /locus_tag="ACHKJF_005372"
     CDS             1264..1866
                     /gene="fimB"
                     /locus_tag="ACHKJF_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP0952540.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2344..>2930
                     /gene="fimE"
                     /locus_tag="ACHKJF_005373"
     CDS             2344..>2930
                     /gene="fimE"
                     /locus_tag="ACHKJF_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP0952541.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKR"
BASE COUNT          870 a          531 c          606 g          923 t
ORIGIN      
        1 attaaatacc tgagtataaa atctcttctg atgtttaatt gatttgaatg ttcgtaagct
       61 atatcactta ctcaatccat tttacccaga gtcataaaat agaatgtctg ttgatctcat
      121 tttcatattc ctttcagact gaagtgcgtt ttcatcaccc gatgagaaag aactgaagtg
      181 atgtttgcca tagattgcga gatgccctgt ctcattgact atcttggtgc acctttatac
      241 ctgttatacc agatcaaaaa tcacgcaatc catacaacaa aaccagattt gcaattcgtg
      301 tcacaaaata tgtcgatatt tttctaagaa gaagatgcca tgtgaagcca gacgaacact
      361 tgcggaggcc ttcacaaacc aaagattgtt gtttaattat ctgttttata agcaatttat
      421 ttaaagtata aacaaggaaa gatttgcaag ctaactcagt gagcgtggtg aaaatcagtg
      481 tttacccgcc atcaggctga gcataattct catcatgaaa tatgtttcct ggtttgtggc
      541 ttgtaactgg tcacttctga agtcgatctg gagaggcttg ttgatgttgg tgttttcagg
      601 atgatgtttc acttagtttg tttgccgtat cgcccggcga atggctgtga ttgaggaagg
      661 tttaagtcgt agtgaccaaa gctatattta ccaacgaatg tagatgaaaa aatcatctcc
      721 tgcgttcccc atatctccag gataaaaagg aatgtaacaa tctcatggcg taagctgacg
      781 aatcagccag gaataatcgc tagggaccta agaattagca tgataatagc cactaagaaa
      841 ttactgcgct ccatgaaata gccattttgt ggcaaatgga gttgactaat aatgtcatat
      901 gtgagacggc tagttgaacg aatattaaat tttgctgaat tttttatgtt gattttactt
      961 gttacagaac atatcacatg atatatagat aagattagtt gcattaatga tgagggttat
     1021 tattagattc gtatccgatt gataaatata taaaggtaca tagcatgcaa gagcatggcg
     1081 tttgtatggc aacgttatta taattaactg ttgctactcc atttaagttc actcagaaga
     1141 actggtccac ttacgttagt tattaagcaa acgttcgctt ttataaacat aatcaggatt
     1201 aaaatgttgg attattgcta acccagcaca gctagtgcgc gtctgtaatt ataagggaaa
     1261 acgatgaaga ataaggctga taacaaaaaa aggaacttcc tgacccatag tgaaatcgaa
     1321 tcactcctta aagcagcaaa taccgggcct catgcagcac gtaattattg tctgactttg
     1381 ctttgtttta ttcatggttt ccgggcgagt gaaatttgtc gattgaggat ttcggatatt
     1441 gatcttaagg caaagtgtat atatatccat cgattaaaaa aaggcttttc aacaacgcac
     1501 ccgctattga acaaagaagt tcaggctcta aaaaactggt tgagtatccg tacttcgtac
     1561 ccgcatgctg agagcgagtg ggtattttta tcgcgtaagg ggaatccgct ttctcggcaa
     1621 cagttttacc atattatctc gacttccggt ggtaatgccg ggttgtcact ggagattcat
     1681 ccgcacatgt tacgccattc gtgtggtttt gctttggcga atatgggaat agatacgcga
     1741 cttatccagg attatcttgg gcatcgcaat attcgtcata ctgtctggta taccgccagc
     1801 aatgcagggc gtttttacgg catctgggat agagcaagag gacgacagcg tcacgctgtt
     1861 ttatagagat agttgagata ccagggatgg tgttaaaaac tacattattc ttacgatata
     1921 tcaaaaatga ttaaccattt attaattgat gttattgtaa gtgtgattct taattatcat
     1981 aataaacatt aagttaacca tatccataca aaatacaatg gtttatgttc ttcaaaataa
     2041 ataaacaaaa tcattcataa atttacacat cacttaaatt ctcctgtttc cgcacttttt
     2101 tctttatttt ttaagcaact ggaagttaat ccactgcaat ctattgttat attgaatcaa
     2161 atcaatgaaa atagatgttg tcacatcagt gatattttat ttttgtatga tatttaatgt
     2221 aattgactga tagccacatc acttcgtgtg tggttatctt tttatctatt gggctaattt
     2281 tgaccgattg aggtttccta taggtattca ttcaaatata tctcagttag gagcactact
     2341 attgtgagta aacgtcgtta tcttaccggt aaagaagttc aggccatgat gcaggcggtt
     2401 tgttacgggg caacgggagc cagagattat tgtcttattc tgttggcata tcggcatggg
     2461 atgcgtatta gtgaactgct tgatctgcat tatcaggatc ttgaccttaa tgaaggtcga
     2521 ataaatattc gccgactgaa gaacggattt tctaccgttc acccgttacg ttttgatgag
     2581 cgtgaagccg tggaacgctg gacccaggaa cgtgctaact ggaaaggcgc tgaccggacc
     2641 gacgctatat ttatttctcg ccgcgggagt cggctttctc gccagcaggc ctatcgcatt
     2701 attcgcgatg ccggtattga agctggaacc gtaacgcaga ctcatcctca tatgttaagg
     2761 catgcttgtg gttatgaact ggcggagcgt ggtgccgata cccgtttaat tcaggattat
     2821 ctcgggcatc gaaatattcg ccatactgta cgttataccg ccagtaatgc tgctcgtttt
     2881 gccggactat gggaaagaaa taatctcata aacgaaaaat taaaaagaga
//
LOCUS       DAZGXZ010000277         2910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000277 DAZGXZ010000000
VERSION     DAZGXZ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.279"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            291..1457
                     /gene="nhaA"
                     /locus_tag="ACHKJF_005374"
     CDS             291..1457
                     /gene="nhaA"
                     /locus_tag="ACHKJF_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP0952542.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1523..2422
                     /gene="nhaR"
                     /locus_tag="ACHKJF_005375"
     CDS             1523..2422
                     /gene="nhaR"
                     /locus_tag="ACHKJF_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP0952543.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2461..>2910)
                     /locus_tag="ACHKJF_005376"
     CDS             complement(2461..>2910)
                     /locus_tag="ACHKJF_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP0952544.1"
                     /translation="NTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQ
                     GEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQY
                     GKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          699 a          640 c          720 g          851 t
ORIGIN      
        1 ggattatttt tgaaatattg tcctaacaaa taacgccgac tgacaagaaa ttaattatta
       61 cttttcctaa ttaatccctc agaaatcctc accttaagct atgattatct gggcttaggg
      121 tcactcgtga gcgcttacag ccgtcaaaaa ctcatctcac cgctgatggc gcaaattctt
      181 caatggatcg taaaaaacga gttattccta cactataatc tgattttaac gatgatttgt
      241 gcggggtaga atagtaaaaa cgatctattc acctgaaaga gaaataaaaa gtgaaacatc
      301 tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt gccgctatcc
      361 tggcgatgat tatggccaat agcggcgcaa ccagtggatg gtatcacgac tttctggaga
      421 cgccggttca gctccgggtt ggttcactcg aaatcaacaa aaacatgctg ttatggataa
      481 atgacgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa cgtgaactga
      541 tgcaaggatc gctagccagc ttacgccagg ccgcatttcc agttatcgcc gctattggtg
      601 ggatgattgt tccggcatta ctctatctgg cctttaacta tgccgatccg attacccgcg
      661 aagggtgggc gatcccggcg gctactgaca ttgcctttgc acttggtgtg ctggcgctgt
      721 tgggaagtcg tgttccgtta gcgctgaaga tctttttgat ggctctggct attatcgacg
      781 atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg atggcctctc
      841 ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc
      901 gcacgggcgt ctatattctg gttggcgtag tgttgtggac tgcggtgttg aaatcggggg
      961 ttcacgcaac cctggcgggg gtaattgtcg gcttctttat tcctttgaaa gagaagcatg
     1021 ggcgttctcc agcgaagcga ctggagcatg tgttacaccc gtgggtggcg tatctgattt
     1081 tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggtgtcacg ctggatggct
     1141 tgacctccat tctgccattg gggatcatcg ctggcttgct gattggcaaa ccgctgggga
     1201 ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct gagggaacga
     1261 cttatcagca aattatggcg gtggggatcc tgtgcggtat cggttttact atgtctatct
     1321 ttattgccag cctggccttt ggtagcgtag atccagaact gattaactgg gcgaaactcg
     1381 gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta cgcgttcgtt
     1441 tgcgtccatc agtttgacag gacggtttac cggggagcca taaacggctc ccttttcatt
     1501 gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt attacttctg
     1561 gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt taactccaca
     1621 aaccattacc ggacagattc gagcgctgga agagcgcctg caaggcaaat tatttaaacg
     1681 caagggacgt ggtctcgaac ccagcgagct gggagaactg gtctatcgct atgccgataa
     1741 aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag aatccaattt
     1801 attgtttgac gttggtgtgg ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa
     1861 cgccgcagtg gtagaaggcg agcccattca tcttcgctgc ttcgaatcca cccacgaaat
     1921 gctgctggag caattaagtc agcataaact ggatatgatc atttctgact gtccgataga
     1981 ctctacgcag caggaaggcc tgttctccgt gagaattggc gaatgtggcg tgagtttctg
     2041 gtgtacaaat ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac ggcgactttt
     2101 gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta actcccaggg
     2161 attaaacgta gaaatcctcg gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc
     2221 gatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt atgccgataa
     2281 aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc
     2341 tgagcggatg attcagcacc cggcggtaca gcgaatctgc aatacggatt attctgcgct
     2401 ttttagtcca gcggcgcgtt aatcggcacc tccccccaaa gttaaggtgg gggagataga
     2461 ttagttgtac ataaccacga ttttgactcg gctcattatt tgcccgcttg agacattatt
     2521 tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat ggggatgctg
     2581 aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat tggagagttg
     2641 aaagcctact tgacccgcac cgtcctcact gttttctaac tcatttttta atatgctggt
     2701 tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acatcactgg gttcaaaaac
     2761 taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact gataattttc
     2821 attattgata cgagacagta caaatcgtcc agtgtcgatg gattgatctt caaaaccaca
     2881 ggtcattaaa tttacctgta aaatagtatt
//
LOCUS       DAZGXZ010000278         2817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000278 DAZGXZ010000000
VERSION     DAZGXZ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.280"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            217..942
                     /locus_tag="ACHKJF_005377"
     CDS             217..942
                     /locus_tag="ACHKJF_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952545.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFIVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            900..1577
                     /gene="bglJ"
                     /locus_tag="ACHKJF_005378"
     CDS             900..1577
                     /gene="bglJ"
                     /locus_tag="ACHKJF_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP0952546.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1615..2403)
                     /gene="fhuF"
                     /locus_tag="ACHKJF_005379"
     CDS             complement(1615..2403)
                     /gene="fhuF"
                     /locus_tag="ACHKJF_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP0952547.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2544..2780
                     /locus_tag="ACHKJF_005380"
     CDS             2544..2780
                     /locus_tag="ACHKJF_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP0952548.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
BASE COUNT          750 a          602 c          702 g          763 t
ORIGIN      
        1 tattttaaat gtctccaggc tatttcacca ggttatcatt tgatgctaac cttctgacac
       61 atgcagtgga gttgttgtgc agcaggagta tgctgatatg aaagtgaatg ctaaggataa
      121 tttattcgct taatctatta atttgctgga aacatttaag gaatgaatat cctgaataaa
      181 ccatcatgat ttttagacag tggatgtgga ggaaatatgt tgccaggatg ctgcaaaaat
      241 ggaattgtta tcagtaaaat acctgttatg caagcagggt taaaagaggt catgaggact
      301 cacttccctg aatatgaaat aatatccagc gcctctgcgg aggaccttac cttattacaa
      361 ttacgtcgtt ccggattagt cattgctgat ttagccggtg aaagtgaaga tcctcgttct
      421 gtttgtgaac attattattc tttaatctca caatatcggg aaattcactg ggttttcatt
      481 gtgtcacgct cctggtattc ccaggcagta gaattgctca tgtgccctac ggcgacgtta
      541 ttgtctgatg ttgaacccat tgagaatctg gtcaagaccg tacgttccgg caatacgcac
      601 gcagagcgta tcagcgccat gctgacctct ccggcaatga atgaaactca tgattttagc
      661 tatcgctccg tcattctcac tctttcagag cgcaaggtac tgcggctatt aggtaaagga
      721 tggggcatca accagatagc ttcgttgctt aagaaaagta ataaaactat cagcgcccaa
      781 aaaaacagtg cgatgcgtcg actggcaatt cacagcaacg ctgaaatgta tgcatggata
      841 aatagcgcgc agggtgcaag agaacttaac ttgccttctg tttatggaga tgccgcagaa
      901 tggaacacag ccgaattaag aagagaaatg tcgcactcat agaaaaatgc gtcatgagca
      961 gtatcggtat tgagagttta ttcagaaagt ttgcgggtaa cccttataag ctccatacct
     1021 ataccagtca ggagtcattt caggatgcca tgtcgcggat ctcatttgcg gcggtcattt
     1081 tttctttttc tgccatgaga agtgagcgca gagagggatt atcttgcctg actgaactgg
     1141 cgattaagtt tccgcgtacc cggcgtttag ttattgcgga tgatgatatt gaagctcggc
     1201 tgattggttc attgtcgcca tcaccgctgg acggtgtatt aagtaaagcg tcaacgctgg
     1261 agatttttca tcaggaactc tttttgtcat taaatggtgt acgtcaggcg accgaccgac
     1321 tgaacaatca gtggtacatt aaccaaagcc ggacgctaag cccgacggag agagaaatat
     1381 tgcgctttat gtcgcgtggc tactcaatga cacaaattgc cgagcagctt aaacgcaata
     1441 tcaaaacgat ccgtgcacat aaatttaatg tgatgtcgaa actgggcgtc agttctgatg
     1501 cagggttgtt ggaggccgca gatattctgt tatgtatgcg gcattgcgaa gcaagtaatg
     1561 tgttgcatcc ctattaatcc gcatgatgcc gggtttactt ccccggcagt gctttcattt
     1621 cagcgtacaa tcgccacatt gctgcacatc cggtaagcga taacgctggc agcaggtccg
     1681 gcgcaccagc aggccgtcgc gtagcaccac ggtacgccag agtggattat cttcaccggt
     1741 agtaagcgtt ttctcaaaaa agagggcatg gcgcagcgat tcaaccgtcg cctcgccgag
     1801 cagttgcttc atctcagtaa gataccagtt gatcaaataa ccggtattac tccagataag
     1861 tttgccgttg atctctccgg tcgcttctag tgcctgcaca accggaacca gcgcctggct
     1921 gattaacctt tccattcgct gctgcggcga atgtggtgtt gcgtttttat cttcacacac
     1981 atcgacccag aaacaggcgg cacggccggt ttcgtgaaac tcagcatgga aatgttccgg
     2041 cgacacatct aatgcctttt cctgcgtcag tagcgccagc attaatggtg gcaccatcag
     2101 gccgatatac cattgtgccc atagtgagat cagcggtttg ttctcgcgga tcatcgttgg
     2161 ttggttgcga tagatatgat cggaatagac cgcgagtaga gaacttagcg cattcggtga
     2221 tgaccattgc gccagcgtca tggcgttaag cggagcgggt tcatccaggc ggataaactc
     2281 cagcaaatgt tcacgatgtt cggcgatcgt cgcacgtacg gcttgtgcaa gcccagcatc
     2341 ctgtggctgg agatgcgttc gccagataac atcttcatag agcggtgcgg aacgataggc
     2401 cataatcggg atagtaatct aaatgataat gattgctaat cctagcgata ggtttacccg
     2461 atagcaaggg atttctctgg cttgcaaatg ataaaaatta tcatatgata ttggttatca
     2521 ttatcaatga aagagatgaa accatgttgc aacgtacgct gggcagtggc tggggagtgt
     2581 tgctgccagg attgctgatt gcagggctga tgtatgcgga tttatcaccg gatcagtggc
     2641 ggattgtcat tctgatggga ttagtattga cgtcggtgat gctgtatcac aaacagttac
     2701 ggcattacgt tttgctacca tcgtgcctgg cgcttattgc tgccatcatg ctgatgataa
     2761 tgaatttgaa tcagggatga aaaatcaagg aagaaacaag aaaggaagta aagataa
//
LOCUS       DAZGXZ010000279         2733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000279 DAZGXZ010000000
VERSION     DAZGXZ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.281"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(366..1352)
                     /locus_tag="ACHKJF_005381"
     CDS             complement(366..1352)
                     /locus_tag="ACHKJF_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0952549.1"
                     /translation="MYRYDFNEKIDFWYVIALAGQSNGMAYGEGIPLPDTLDKPESRV
                     KQLARRKTITPGGKECKFNEIIPADHCLHDVQDMSGYHHPAADLHKGEYGCVGQGLHI
                     AKKLLPYIPEQAGILLVPCCRGGAAFTVGAEGMYVPDTGATADAMRWGTGTALYEDLV
                     ARVKVALEYNRKNKLLSVCWMQGEFDLMSPDYEKHPDLFYQMVTSFRSELSEYSSQCV
                     GNSSERVPWLCGDTTWYWKESYQKEYDFIYGHYRQRTDDEIHFLSFQDSNRHELTNEP
                     EEDADDLSVGYLGSSWRTELSWTTSQRSTHFNSMARRGVIAECYAQKIRNYL"
     gene            1453..2187
                     /gene="nanR"
                     /locus_tag="ACHKJF_005382"
     CDS             1453..2187
                     /gene="nanR"
                     /locus_tag="ACHKJF_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0952550.1"
                     /translation="MSDNKKTVLPGLTTTRKPIVTKKLSEIVAEELERMIRQEEIREG
                     EYLPSERELMAFFGVGRPSVREALSSLRHKGLIRYNKGEKASVCRPSPETIINGLSGI
                     VKDFLSTADGIKDFERLRIFFETNLVRYAAKNATPEDIHRLEKVLSIHHNSLRTPEVY
                     AQYDIEFHSILADIPDNKVLKTIHSAFSEWLIMAKPLEKNKMTYENNKKTHQQHIEIL
                     TAIKEHDCDKAEQLMYNHLYKRIEAS"
     gene            complement(2223..>2733)
                     /locus_tag="ACHKJF_005383"
     CDS             complement(2223..>2733)
                     /locus_tag="ACHKJF_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0952551.1"
                     /translation="NADKWHLQRHRIGVLGFSAGGHLASTFVNQICDFKRSSDSKEQE
                     IFAIPDFVGLIYPVISMKDDVTHPDSRKQLLGDNSTSENIIQYSADLNVTSCFPPVFL
                     LHCCDDDLVSIENSLLMYRGLIEKGVSAEMHLYEKGGHGFSVQWATDLPVGGWIKLFM
                     DWMYSHGFK"
BASE COUNT          856 a          622 c          496 g          759 t
ORIGIN      
        1 acctgcgcca catcgactga atgttttggc cctgacagaa aaaagaacta ataggtgatg
       61 tttccgtcca gagatactct ggatggagtg tcggcgtcag tgatataaga ctcgagtgaa
      121 cactgagccg acctcgaaat gacgttgtag gtgcagacat gccataatgg gactgtcgat
      181 gatattacag ggactcacgt ccctggtgcg tgccttcgtg acacactacc gtccacatag
      241 tatcagaacc agtgatatca aacggtaacg accagctatc ttggtgttga taccgacaac
      301 tccataccac tccgtcatgt ctctcagaga atcatgtcag agctgtagat atatcctgaa
      361 cttgtctaca aataatttct tattttttgc gcataacatt cagcaataac tccccttctg
      421 gccattgaat taaagtgtgt acttctctgt gacgttgtcc agcttaattc tgtcctccat
      481 gatgacccca gatacccgac agataaatca tcggcatctt cttctggctc attagttaat
      541 tcatgtcggt tactatcctg aaagctgaga aaatgtattt catcatcagt ccgttgtctg
      601 taatgtccat atataaaatc atattctttt tgataagatt ccttccagta ccaggttgta
      661 tcaccacaca accatggaac tctttctgac gaattaccaa cacactgaga tgagtattca
      721 gataactcag acctgaaaga agttaccatc tgataaaaca agtcaggatg cttttcataa
      781 tctggtgaca taagatcaaa ctccccctgc atccagcaga cagatagcag tttattcttt
      841 ctgttatatt ccagagctac cttaacccgg gctactaagt cctcatagag tgctgttccg
      901 gtgccccacc gcattgcatc agctgttgcc cctgtatcag gtacatacat accttcagct
      961 ccaacagtaa aggcggcacc tcctctgcaa caaggaacca gtaatatccc tgcttgctca
     1021 ggtatgtatg gcagtaactt tttagctata tgcaatccct gtccgacaca accatattcc
     1081 cctttatgca aatcagcagc cggatggtga tatccactca tatcctgaac atcatgcaga
     1141 cagtgatctg ccggaataat ctcattgaat ttacactctt ttcctcctgg tgtgattgtt
     1201 tttcgacgag ccagttgttt tacacgggac tcgggtttgt cgagtgtatc tggcagggga
     1261 ataccttcac cgtaggccat accattagac tgtcctgcga gggctatcac ataccagaaa
     1321 tctatttttt cattaaaatc ataacgatac atcactcccc cctttacata atagatttaa
     1381 ttaaatattt tcattattaa aagaaagtgt taaattaaaa actcatttgc tctaatccat
     1441 ataaatcaat atatgtccga caacaaaaaa accgtgttac ctgggctaac aacgaccaga
     1501 aagcccattg ttacaaaaaa actttcagaa atcgttgccg aagagcttga aagaatgatc
     1561 cgccaggagg agataaggga aggtgaatac ctgccttctg aacgtgaact tatggcattc
     1621 tttggcgttg gaagaccatc tgtcagagag gctttatcat ctctcagaca taaagggcta
     1681 atccggtata acaagggaga aaaagccagt gtctgccgtc catcaccaga aaccatcatt
     1741 aatgggttat caggcattgt taaagatttt ttatccacag ctgatggcat caaggatttt
     1801 gaaagactac gcatcttttt tgaaaccaac cttgtaagat atgcagcaaa aaatgcaacg
     1861 cctgaggata ttcacaggct tgagaaggtg ctcagtatcc atcataactc actgagaacc
     1921 cccgaagtgt atgctcaata tgatattgaa tttcatagta tacttgctga tatacctgac
     1981 aataaagtat taaaaacaat acactcagca ttttctgaat ggctgataat ggccaaacct
     2041 ctggaaaaga ataaaatgac atatgaaaat aacaaaaaaa cccatcagca gcatattgaa
     2101 atacttacgg ccataaaaga gcatgattgt gataaagctg aacagctaat gtataatcac
     2161 ctttataaac gaattgaagc atcatagtta ctcgacgctt caccaggtag tcaccataaa
     2221 tattatttaa atccatgaga atacatccag tccataaaca gttttatcca tcctccaaca
     2281 ggaagatctg tggcccactg aacactaaag ccatgccctc ctttctcata gagatgcatt
     2341 tcagcagaaa cgcctttctc tattaggcca cggtacatga gcagactgtt ttcaattgac
     2401 acaagatcat catcacaaca atggagtaaa aacacaggag gaaaacatga tgtgacatta
     2461 agatccgcac tatattgtat tatattttct gaggtactat tgtcccccag taattgcttc
     2521 ctggagtctg gatgtgtcac atcatctttc attgatatta caggataaat taacccgaca
     2581 aaatcgggaa tagcgaaaat ctcttgttct ttactgtcag aagaacgttt gaaatcacaa
     2641 atctgattca caaacgttga ggcgagatgc cccccagcag aaaatcctaa tacaccaata
     2701 cgatgacgct gtagatgcca tttatcagca ttc
//
LOCUS       DAZGXZ010000280         2731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000280 DAZGXZ010000000
VERSION     DAZGXZ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.282"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHKJF_005384"
     CDS             complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHKJF_005384"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP0952552.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHKJF_005385"
     CDS             complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHKJF_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP0952553.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          694 a          666 c          649 g          722 t
ORIGIN      
        1 tgttgcgttt gtcatcagtc tcagcccgct tttcagcggg cttcattgtt tttaatgctt
       61 acagcactgc cacaatcgct tcgcacagcg gagccatgtt atctggtgtc atcccggcca
      121 catttacgcg accagaagca accgcatata cgccaaactc ttcgcgcaga cgcagcactt
      181 gttcttttgt caggccactg aaggagaaca tgccgttctg tttgatgata aagctgaagt
      241 cgcggtttgc gcctttttcc tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac
      301 gctggcgcat atcagtcagc tcttgttccc aaatcgcacg taacgcatcg ttgctcagga
      361 tggtggcaac aacagaagcg ccgtgtgctg gtgggttaga gtagttagcg cgaatcgccg
      421 ctttcatttg gctgaatgcg cgatcaacgg tttcactgtc ggcagcaacc agagtacaag
      481 cgccaacacg ctcgttgtac aggccaaagt ttttagagta ggaactggca acaatcagct
      541 ctttatgcat agccgcgaaa gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa
      601 aaccctggta agcgaagtca aacagcggta accagccttt ctcaacggag agttgtgcca
      661 gtgtctgcca ttgttccagc gtagggtcga taccggttgg gttatggcag cagccatgga
      721 acagcactac gtcgccagcc tgagcttcat tcaggctgtt aatcagtgca tcgaagtcaa
      781 gagtgtgatt ttccgcatca taataagcgt attcacgaac ttccagacct gcagagttaa
      841 agacgctctt atggttcggc cagcttgggt tgctcaccca cactcgctta acgctggtat
      901 tttttgccag gaaatcggca gctacgcgta gtgcgccagt gccccccgga gtctgtgccg
      961 tgcgagcacg tttgtcattg atcagggcgc tacctttacc aaacagcagt tcctgagtgc
     1021 agcgaccaaa ttcagggatg ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga
     1081 gcagatactg ttcagccttt ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt
     1141 tatagacacc aatcccgagg ttaattttgc cgggacgttc atcggcacga aacagatcgg
     1201 ccaggcccag aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc
     1261 attatggtta cagaagggaa gtccgctatc agggtaacgg gagatttaca aaattccaac
     1321 tattactgat gaaaacgcag gctgtttttg caagacgtga gattgctctg gaaggtataa
     1381 aaaaaacagg accaaagtcc tgttttttcg gcatttaaca aagaggtgtg ctattagaac
     1441 tggtaaacga tacccacagc aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa
     1501 tcgatctggt tgatgatgta gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt
     1561 gcgcccactt caaagtagtt caccagatca acatcaccga taccttctac gtctttcgct
     1621 ttagatttgg tgtaagcgat ggacggacgc agaccgaaat cgaactggta ttgcgcaact
     1681 aacagaacgt cttgcgtttt gttggcgaag ccgctggtgt ttgtaaattt attagtgatc
     1741 ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt cgcgtcgtac
     1801 ttcagaccag tagcccactg ttcagctttt ttaccgttgc caagaggttg agcttcttgc
     1861 aggttggtac ggtcagctgc accataagca ccaacgatac caaagccttc gtattcgtag
     1921 ctgatagaac cgccaacacc gtcgccgtta gaacggcgtg cagtgtcacg ctcgttttta
     1981 cccaggtact gaacagcgaa gttcaggcca tcaaccagac caaagaagtt ggagttacga
     2041 taggtagcaa cgccgccaac acgaccaacg aagaagtcat cgctgtatgc agtatcacca
     2101 ccaaattctg gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg
     2161 ccgtaatcga aagaaccaac gtcagcgtat ttaagacccg cgaatgccag acgcgttttg
     2221 ttaccagttt gagcgtcagc gccttcagag ttgttaccct ggaagttata ttcccactga
     2281 ccataaccgg tcagatcgga attgatttga gtttcccctt taaaaccaag acgggcatag
     2341 gtcatgtcgc cattgccacc gtaactgttt tcaccgttac ccttggaaaa ataatgcaga
     2401 ccaacagctt taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg
     2461 tttgcagtac ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc
     2521 attatttatt accctcatgg ttttttttat gacacctgcc actgccgtca ataagttctg
     2581 tcaataaaaa tttacggaac tattgatgag agtttggtgt ctttatgtgt ctgcaggcat
     2641 ctttccattc aaactaacgt ttcgctaccg tgaaagtgct acaaagataa agatttagtt
     2701 tcaaaaagaa aaaatatgta accaaaagta a
//
LOCUS       DAZGXZ010000281         2721 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000281 DAZGXZ010000000
VERSION     DAZGXZ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.283"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2379
                     /locus_tag="ACHKJF_005386"
     CDS             <1..2379
                     /locus_tag="ACHKJF_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952554.1"
                     /translation="PSDVVWPYTNSDVVVDDNGVGFRYSFSALKDRHTAVEVNYTDPQ
                     NGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTELLETQTVDFT
                     LGSQGLRHTPGDIIEICDNDYAGTLTGGRILSIDAASRTLTLDREVTLPEAGTSTVNL
                     INGSGKPVRVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSIRENTD
                     GTFAITAVQHVPEKEAIVDNGARFEPLSGSLNSVIPPAVQHLTVEVSASDGQYLALAK
                     WDTPRVVKGVRFSLRLTSGSGENSRLVTTAITADTEHRFSGLPLGEYTLTVRAINSYG
                     QQGEPATTAFRINAPAAPASIELTPGYYQITAVPVLAVYDPTVQSEFWFSEKRITDMA
                     QVETFARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAEGYL
                     GLFREKIGKLHLAQGMWELIDNSQLDDEMAEMKTTITETRNEITQTVSKTLEDQSATI
                     QQIQRVQTDTNNDLAALYMLKVQKTKNGIPYVAGIGAGIEDADGQPLSNILLQADRIA
                     MINPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNAD
                     ISGHISANSGTLNNVVIAENCTINGTLKAENIIGDLVKCAGVAFPVDGSYLANGTRTL
                     TVYDDHSFDRQIIIPPIIYVGSKQESRTSNDIWTECFLHVDQNGRRIYSGRSVTEPGI
                     FSGIIDMPAGHGHITLSFTVSSRRQNGSFGSSRISNLQAIVVKKNSAGISIR"
     gene            2578..2718
                     /locus_tag="ACHKJF_005387"
     CDS             2578..2718
                     /locus_tag="ACHKJF_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0952555.1"
                     /translation="MSQKPLKTTVICITVVLIIWITHTSLCEFRFRIAGAEIAAFLQC
                     KQ"
BASE COUNT          644 a          714 c          835 g          528 t
ORIGIN      
        1 ccgtcggatg tggtgtggcc gtacaccaac agcgatgtgg tggtggatga taacggcgtg
       61 gggttccgct acagcttcag tgccctgaag gaccggcaca cggcggtgga ggtgaattac
      121 accgacccgc agaacggctg gcagacttcc acggaactgg tggaagaccc ggacgccatc
      181 ctgcgctacg ggcgcaatct gctgaagatg gatgcgtttg gctgtaccag ccgcggtcag
      241 gcccaccgtg ccggactgtg ggtgataaag acagaactgc tggaaacgca gacggtggat
      301 ttcacgctcg ggtcacaggg gctgcggcac acgcccggtg acattattga aatctgtgat
      361 aacgactacg cagggaccct gaccggcgga cgcatcctgt ccatcgatgc cgccagccgt
      421 accctgacgc tggaccgtga ggtgacactg ccggaagcag ggacatcgac ggtgaacctg
      481 attaacggca gcggtaagcc ggtgcgcgtg gacatcactg cacaccccgc cccggaccgg
      541 atacaggtca gtaccctgcc tgatggtgtg gagacatacg gggtgtgggg actctccctg
      601 ccgtcactgc gccgtcgcct gttccgctgt gtctccatcc gggaaaacac ggacggcacc
      661 tttgccatca cggcagtgca gcacgtaccg gaaaaagaag ccatcgtgga taacggggcc
      721 cgctttgagc cgctctccgg ctcactgaac agcgtcatcc cgccggcagt gcagcacctc
      781 actgtggagg tgagtgcctc agacggccag tatctggcgc tggcgaaatg ggacacgccg
      841 cgggtggtga agggcgtgcg cttcagtctg cgcctgacca gtggcagtgg tgaaaacagc
      901 cgcctggtga ccaccgccat cactgccgac acggagcacc gtttcagtgg cctgcctctg
      961 ggggaataca ccctgacggt cagggcgata aacagctacg gccagcaggg cgaacctgcc
     1021 accaccgcgt tccggattaa tgcgcctgca gcaccggcca gcattgagct gacgccgggg
     1081 tattatcaga taacagcagt accggtgctg gcggtgtatg acccgacggt acagtctgaa
     1141 ttctggttct cagaaaaacg catcacggac atggcacagg tggaaacctt tgcccgttat
     1201 ctggggacag gcagccagtg gagtgtctcc ggcccgcaca ttaagccggg gaaggatttc
     1261 tggttttatg tgcgcagcgt caacctggtg gggaaatctg cgtttgtgga agccagtggc
     1321 cgggccagca atgatgcgga agggtatctg gggctgtttc gggaaaagat aggaaaactg
     1381 catctggcac aggggatgtg ggagctgatt gataacagcc agcttgatga tgagatggcg
     1441 gagatgaaga ccaccatcac agaaacccgc aatgaaatca cacagacggt cagtaaaacc
     1501 ctggaagacc agagtgccac catacagcag atacagcggg tgcagacaga cacaaataac
     1561 gacctggctg cgctgtacat gctgaaggta cagaaaacga aaaacggcat tccgtatgtt
     1621 gccggtatag gtgcggggat tgaggatgct gatggccagc ccctgagcaa tattctgctg
     1681 caggcggacc gtatcgcgat gattaacccg gagaacggca acaccacgcc gctgtttgtg
     1741 gcgcagggga atcagctgtt catgaacgat gtgttcctga agcggttgtt tgcagtgagc
     1801 atcacctcgt ccggcaatcc cccgacgttc tccctgacgc cggagggcag gctgacggcc
     1861 cgcaatgcgg acatcagcgg acatatcagt gcgaactcgg gcacgctcaa taatgtcgtg
     1921 atagcggaga actgtacgat aaatggcacg ctgaaagcgg agaacattat tggtgatctt
     1981 gtgaaatgtg caggggtggc ttttccggtg gatggtagtt accttgcgaa cggtacacga
     2041 acgctgacgg tgtatgacga tcacagcttt gaccggcaga ttataatccc gccgataatc
     2101 tatgtcgggt caaaacagga atcccgtacc agtaatgaca tctggacaga gtgcttcctg
     2161 catgttgatc agaacggacg ccggatttat tccggcaggt cagtgacaga gccgggaatt
     2221 ttcagcggga tcatcgatat gccagccggt catggtcata tcaccctgag ttttaccgta
     2281 agttcacgac gtcagaacgg gagttttggc agttcacgta tcagtaacct tcaggcgata
     2341 gtggtgaaga aaaacagcgc ggggatcagc atccgctgaa catcgcgccc cgggattgcc
     2401 ggaaggagac aaaaaccgta cagtatgcgc gggtgccttt ggctgatggc cggagggaac
     2461 acctgaaggc cggatgtgaa aaggccccgg acaaacattc atgtttaacc cgaggcctga
     2521 ctttcgatct tcagcaaatt gaaggttagc gcctctccgt aaaaggagca agcgtttatg
     2581 tcgcaaaaac cgttaaaaac caccgtgatt tgtatcacgg tagtgctcat tatctggatc
     2641 acccacactt cactgtgcga gttccggttc cggatagcgg gcgcggagat tgcggcgttc
     2701 ttacagtgta agcagtaaga a
//
LOCUS       DAZGXZ010000282         2711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000282 DAZGXZ010000000
VERSION     DAZGXZ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.284"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..802
                     /locus_tag="ACHKJF_005388"
     CDS             <1..802
                     /locus_tag="ACHKJF_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0952556.1"
                     /translation="IVRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGL
                     SLCIRPGERVGIIGSIGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVF
                     LLEQNPRLFLGTLRENLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPRGLDMQTGEG
                     GQGLSGGQRQMVALTRMVLGSPRVVLLDEPTTGLDLQTESVVLKGLSHWSRGRTMVVV
                     THRLQVLNIVDRIVVMNEGRVMMDGPRDEILKKLSGKQQSEESRQAAS"
     gene            862..2109
                     /locus_tag="ACHKJF_005389"
     CDS             862..2109
                     /locus_tag="ACHKJF_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFP0952557.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKSLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDNVPEDLRERESTV
                     YFLRKTELAQSIAGLKQSKALLDREIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKRLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            2395..>2711
                     /locus_tag="ACHKJF_005390"
     CDS             2395..>2711
                     /locus_tag="ACHKJF_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952558.1"
                     /translation="MKMIFTGKVSGEKTVLTAGARHTVKAQAGEQYGLVDEVTGLVPD
                     GVEADRSGDDLILRKKEDDTEIRIEGFWEECQPGETQCTAVFNVVGENGQVTEAVLTQ
                     DGPV"
BASE COUNT          737 a          527 c          804 g          643 t
ORIGIN      
        1 cattgtccgc cgtccggtgg agcgggaaag tcagacacat tatctgacag tgaaggatat
       61 ccagggagag tttcgtttca gtgatgtctc cttttcttat aagcccggat tacccgacgc
      121 attgtgcggt ctgtcgctct gtatccggcc cggagagcgg gtgggtatta ttggctcaat
      181 aggttgtggt aaatcgacgc tgctgaaaat gatggtgggg ctgtaccgtc agaacagggg
      241 acaaattact cttgacgggg ttgcgatgca gcaactggac ccctgttttg tccgggataa
      301 agtgtttctt ctggaacaga atccccggct ttttctggga actctgcgtg aaaatctgca
      361 gctttcgcgt ctgccgggaa gcctgtcgga tgtggatatg attaatgcag ccggacgttt
      421 cgggtttgac cgttttatcc ggcatcatcc ccgcggactt gatatgcaga caggggaagg
      481 aggtcagggg ctgtcaggag gacagaggca gatggttgcc ctgacgagaa tggtactggg
      541 ttctccccgg gtggtactgc ttgatgagcc cacgacaggt ctggacctgc agactgagtc
      601 tgttgttctg aaaggtctga gccactggag ccgtggacgc acaatggtgg tggtgacaca
      661 tcgtctgcag gttctgaata ttgttgaccg tattgtcgtg atgaatgaag ggcgggtaat
      721 gatggacggt ccccgtgatg aaatactgaa aaaactgtca gggaaacagc agagtgaaga
      781 gagcagacag gcagcttcct gaacgtcaac cggataagaa aaaagagcag atatgtttaa
      841 cagtttgtgg accgcaggga tatgacagaa aaaacggaac ggaataaagc gtcgtcagag
      901 gtggtcaggc agtcagacct ggcgctgttg acagatgtac aggcagccct tcagcaggaa
      961 aagcatcatg cccttttcct gatggtgatt tttctgctgg tactggtagt gacgtttgtt
     1021 atctgggcat ggaacagtcc tctggatgaa gtgacccgag gtcaggggag tattattccc
     1081 ggcagcaggg agcaggttat acagaccctt gacccgggta tactgaaatc acttgaggtc
     1141 agggaaggcg atattgttga gaaaggtcag gtactgctga cactggatga tacccggagc
     1201 tctgccatgc tgagggaaag tgaagcccgg gtgaataacc tggaggccgt cagggcaaga
     1261 ctccgtgcag aagcttattc cgaatcactg acatttccgg ataacgttcc tgaggatttg
     1321 cgggagcggg aaagtacggt ctattttctc cggaagacgg agctggcgca gagcattgcc
     1381 ggtctgaagc agagtaaggc gctgctggac agggaaatag caatgacgcg gccaattgtg
     1441 cgtgaagggg caatgtcgga ggtcgaactg ttacgtatgc agcgtcagtc agcggagctg
     1501 cagttacaga tggatgagaa acagaataaa tacctgacgg aagccggtgc tgaactggtg
     1561 aagacagaag cagagcttgc tcaggcaaaa gagaatatgg ctggcagagc cgacccggtt
     1621 gagcgttcac gtctcagggc ccccctgaga ggtatcgtaa aaaatatacg ggtaaacaca
     1681 ctgggagggg tggtttctgc aggacaggac ataatggaaa ttattccgct ggaagaccag
     1741 ttgctgattg aagcttatat aaatccacgg gatgttgcct atgtcagaac agggatgccg
     1801 gcacttgtaa aactgacggc ttatgactat gccatttatg gtggtcttga cggcgtggtg
     1861 acactggtaa gtccggatac tttacgtgac cagaaacggc cgggagattt aaaactggac
     1921 ccgaatgaag cttattaccg ggtactggtt accaccagta ataattatct gacagacagg
     1981 aacggaaaaa gattgccagt tattcctggc atgatagcca gcgtggatat aaaaaccggg
     2041 cgtaaaacgg tatttcagta tctgattaaa ccgataacca gaatgaagca ggcacttcag
     2101 gaaagataat aaaaaggtct tccggggaaa gtattttaaa tcaattttaa acgggcagaa
     2161 tgatttcact ttattctgcc cgggtcattg cgcatgcctg tatgtgattt ggggctgaag
     2221 ataatataaa tgttatatct gtcaggggaa aataaatctg aaaaaagcgg aatgattaag
     2281 gaggtccggt cagagaaata aattatctgt aatctgattc gtttttattt aatgactccc
     2341 tttagcggat aaaacataag agcatactgg ttttattaac ctgaggatac ttatatgaaa
     2401 atgattttca ccggcaaggt cagtggagaa aaaacagtac tgacagccgg agcccgtcat
     2461 acagtaaagg cacaggccgg ggaacagtat ggactggttg atgaggtgac aggtctggtc
     2521 ccggatggtg tggaagcgga ccgttctggt gatgatttaa ttctgcgtaa aaaggaagat
     2581 gatacagaaa tccggattga gggattctgg gaagaatgcc agccgggtga aacacagtgt
     2641 acagcagtat ttaatgttgt gggtgaaaat ggtcaggtaa ctgaagctgt cctgacccag
     2701 gatggccctg t
//
LOCUS       DAZGXZ010000283         2589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000283 DAZGXZ010000000
VERSION     DAZGXZ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.285"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            341..484
                     /locus_tag="ACHKJF_005391"
     CDS             341..484
                     /locus_tag="ACHKJF_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP0952559.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            497..706
                     /gene="cspE"
                     /locus_tag="ACHKJF_005392"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             497..706
                     /gene="cspE"
                     /locus_tag="ACHKJF_005392"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0952560.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            872..1681
                     /gene="rlmA"
                     /locus_tag="ACHKJF_005393"
     CDS             872..1681
                     /gene="rlmA"
                     /locus_tag="ACHKJF_005393"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP0952561.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1678..2244)
                     /gene="mntP"
                     /locus_tag="ACHKJF_005394"
     CDS             complement(1678..2244)
                     /gene="mntP"
                     /locus_tag="ACHKJF_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP0952562.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          702 a          601 c          615 g          671 t
ORIGIN      
        1 ttaactgact gttttatgag aaaaagtgat ataacttttt attcattgca tagcaaaaaa
       61 tgtgatattg cacgcactat gtaataactt ctcccactgg cctggaacaa ctgaacttat
      121 tgaactatgt tagaaaatac gccagtttaa gtatctgcct gaactggcaa ggttaagcac
      181 aatgatatat cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga agacgtatcg
      241 agatttgtgc gtctgatcga gacatgttta aaaatggctt gccataatta acgttgtatg
      301 tgataacaga tttcgggtta aacgaggtac agttctgttt atgtgtggca ttttcagtaa
      361 agaagtcctg agtaaacacg ttgacgttga ataccgcttc tctgccgagc cttatattgg
      421 tgcctcatgc agtaatgtgt cagttttatc tatgttatgc ctgcgggcga agaaaacaat
      481 ctaaggaatt tttcaaatgg caaagattaa aggtcaggtt aagtggttca acgagtctaa
      541 aggttttggc ttcattactc cggctgatgg cagcaaagat gtgttcgtac acttctccgc
      601 tatccagggt aatggcttca aaactctggc tgaaggtcag aacgttgagt tcgaaattca
      661 ggacggccag aaaggtccgg cagctgttaa cgtaacagct atctgatcga atccactgat
      721 ctgaagtgtg aatacgcttc aatctcgcta taaagcctcg tcgaatgcga ggctttttac
      781 tatgctttat cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt cgcgttacac
      841 ttgcggcctt tagtatcctg ccggagttgt catgtcgttt tcctgtccac tttgccatca
      901 gcctctttcg cgtgaaaaaa acagctatat ctgtccccag cgacatcagt ttgatatggc
      961 gaaagaaggg tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg atccgggcga
     1021 cagcgcggaa atgatacaag cacgccgcgc attcttagat gccggacatt atcagccgct
     1081 gcgtgatgca attgtcgccc aactgcggga acggcttgat gaaaaggcca cggcggtgct
     1141 ggatattggc tgtggtgaag ggtattacac acacgcattt gccgatgtgt tgcccgaaat
     1201 caccacgttt ggcttggatg tctcgaaggt agcgataaaa gcggcggcga aacgctatcc
     1261 gcaggtcact ctttgtgtcg cttccagcca ccgtttgccg ttttccgata ccagtatgga
     1321 cgccataata cgtatttacg cgccgtgtaa agcagaagaa ttagcacgag tagtgaagcc
     1381 cggcggctgg gtcattactg ccacgccggg accgcgacat ttgatggagc tgaaggggct
     1441 gatttacaat gaagtacatc ttcatgcacc tcatgcagaa caactggaag gttttacatt
     1501 acagcagagt gatgagttgt gttatccgat gcgtcttcgc ggtgatgaag ccgtcgcatt
     1561 attgcagatg acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa cactggcagc
     1621 aaaagaagtg ttcgactgcc agacggactt taatattcac ctctggcagc gttcttatta
     1681 accgtggaag tgcgtccaga ggatctggac gccgatgccg atcagcacca gcccgccgag
     1741 aatttccgct tttttcccaa taattgagcc gataaagcga ccaaccatca tccctaatgt
     1801 cgacataatc aaggttgcac aaccaatggc caatgcggtc gcgataatgt tgacctgcag
     1861 gaaagcaaga ccaacaccca cagccatggc atccaggctg gtggcaatcg cggtggttac
     1921 cagtagccag aaaccgtgcc gacggcgcgg ctcttcatct tcatcatctg cgccacgaaa
     1981 accctcaata atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa tccagtggtt
     2041 ccattcaagg acaaaccggc tggctaacat gcccattccc cagccgatca gcggcgtcag
     2101 ggtttcgacg gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt tcggtttatg
     2161 gagggtggca cctttaccga ttgatgcagc aaatgcatcc atcgacatac caaacgcaag
     2221 aagaacagta gcagtgatat tcatgacaat gtcctgaccg gggtatccat aaaacacatc
     2281 atcgccccca gcaaacatgc atacatgaac aacaagccgt gatggttttc gcgttcatgc
     2341 aggcatagcg attgatgtgc atatggtctc gcctgactga aagtattcag tccgtacgtg
     2401 ccacgttttg caacgagtat gttgacacgt acatttccgg aatctggaaa tcggctactc
     2461 cccaaatgag ggcgcaacct taacatattt tgagaatata aaacaactgt tggaatatat
     2521 tatttcattg ctttattgat aacgattttc atttagattt atatatcaat gaaacgttaa
     2581 aataaataa
//
LOCUS       DAZGXZ010000284         2575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000284 DAZGXZ010000000
VERSION     DAZGXZ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.286"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1125
                     /gene="traB"
                     /locus_tag="ACHKJF_005395"
     CDS             <1..1125
                     /gene="traB"
                     /locus_tag="ACHKJF_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFP0952563.1"
                     /translation="QRRIEKLGQENAALAEQVKALGANPVTVTGEPVPQMPASPPGPE
                     GEPQPGNAPVAFPPQGAGAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKTFSYDA
                     GTKQGPSLPYIPSGSFAKAILIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDA
                     TGCFVGLEAWGDVSSERAIVRTRNISCLKEGKTIDMPVKGHVSFRGKNGIKGEVVMRN
                     GKILGWAWGAGFVDGIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLS
                     DYYIKRAEQYHPVIPIGAGNEVTVVFQDGFQLKTVEEMALEQAQGQAEESNPGSPVPN
                     PPSAESHLNGLNTDQMLKQLGDLNPQQFMSGGQEGGNDGK"
     gene            1115..1702
                     /gene="traP"
                     /locus_tag="ACHKJF_005396"
     CDS             1115..1702
                     /gene="traP"
                     /locus_tag="ACHKJF_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFP0952564.1"
                     /translation="MANNTSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVQEINFVRQTATAGQFPVSECRFSSSDLPGAEEKEICHYRNAG
                     ATEYARETDRSLMQLVTAFWATLALMYMSFAAGTRKYPMWPGKMKCVRGGTADERLRE
                     VLAEDASLPGKISKCHAYFPDNRTNRHNGDKNEHA"
     gene            1689..2009
                     /locus_tag="ACHKJF_005397"
     CDS             1689..2009
                     /locus_tag="ACHKJF_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFP0952565.1"
                     /translation="MNMRNIHVITALSVPDKRISDDFMHAVLSNCTTGILLPSQEKFS
                     PELLPRNFNMAAVGVMKKGMSMSLFSSLGHIVVAGQSGGGKSVPVKELIDRIIQKYKE
                     TFDE"
     gene            2002..2253
                     /gene="trbG"
                     /locus_tag="ACHKJF_005398"
     CDS             2002..2253
                     /gene="trbG"
                     /locus_tag="ACHKJF_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFP0952566.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGEERIVA
                     YVYKSSRNTVFENPDIVKTCTVRDLKNDFMNCDEKGEGQ"
     gene            2250..>2575
                     /gene="traV"
                     /locus_tag="ACHKJF_005399"
     CDS             2250..>2575
                     /gene="traV"
                     /locus_tag="ACHKJF_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFP0952567.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLADGNFRTTPVQAVAATTPSRSRPAVTALPEQKLLAPR
                     PLFTAAR"
BASE COUNT          642 a          630 c          751 g          552 t
ORIGIN      
        1 cagcgtcgca ttgaaaagct ggggcaggag aatgctgcgc tggcagagca ggtgaaagcc
       61 ctgggcgcga atcccgttac agtgaccgga gagccagttc cacagatgcc ggcatcccct
      121 cccggtccgg agggggagcc tcagcccggt aatgcgcccg ttgcttttcc gccgcagggg
      181 gcaggtgcag tgccaccgcc caccgcgttc tatccgggca acggagtcac gccgccgcca
      241 caggtgacgt accagtctgt gccggtgccg aaccggatac agcgtaaaac gttcagctat
      301 gatgctggca caaagcaagg tccgtccctg ccgtacattc cgtcaggcag ttttgcgaaa
      361 gccattctga ttgaaggggc ggatgccaat gcctctgtca ccggtaatga atccacggtg
      421 ccgatgcagt tgcgtatcac cggtctggtg gaaatgccga acagcaaaac ctatgacgca
      481 acgggatgct ttgttgggct ggaagcctgg ggggatgtgt ccagtgagcg tgccatcgtg
      541 cgtacccgca atatcagttg cctgaaggag ggtaaaacca tcgatatgcc ggttaaaggg
      601 catgtcagct tccggggtaa aaacggtatc aagggtgaag tggtgatgcg taacggcaaa
      661 atcctcggct gggcatgggg cgcgggattt gttgacggta tcggtcaggg aatggagcga
      721 gcttcacagc cagctgtcgg gctgggagcc actgccgctt acggggccgg cgatgtcctg
      781 aaaatgggga tcggtggcgg tgcatcgaaa gcggcacaga cactcagtga ctactacatc
      841 aaacgcgctg agcagtatca cccggtgata cccatcgggg cgggtaatga agtgaccgtg
      901 gtgttccagg acggtttcca gctgaaaacc gtggaagaga tggcgctgga gcaggcacag
      961 gggcaggcgg aagagagtaa cccgggaagt cccgtgccga atccgccatc cgcagagagt
     1021 cacctgaatg gtctgaatac cgaccagatg ctgaagcagc tgggcgacct gaatccgcag
     1081 cagtttatgt ccggtggtca ggaagggggc aacgatggca aataatacgt cgtcccgtca
     1141 ggcgtgccat gccgcccggt atgttgtcgc ccgggttctg cgtggactgt tctggtgtct
     1201 gaaatacacc gtcattctgc cactggccac aatggccctg atggcgttat ttgttttatg
     1261 gaaggacaac accacgccgg ggaaattact ggttcaggag attaactttg taagacagac
     1321 agccactgcc gggcagtttc cggtcagtga atgccggttt tcttcctcag accttcccgg
     1381 agcagaagaa aaagaaatct gccattaccg caatgctgga gccacagagt atgccaggga
     1441 gactgaccgg tcactgatgc aactggtcac cgcattctgg gcaacgctgg cacttatgta
     1501 tatgtcgttt gcggcaggca ccagaaaata tccgatgtgg cccggaaaaa tgaaatgtgt
     1561 ccggggggga accgcagatg aacgactgag ggaagtactt gctgaagatg cttccctgcc
     1621 gggaaaaatc agtaaatgcc atgcttattt ccctgataac agaacaaaca gacacaacgg
     1681 agataaaaat gaacatgcgt aatattcatg ttattacggc cctctctgta ccggataaac
     1741 gcatatcaga tgattttatg catgctgttc tcagtaactg caccacagga atcctacttc
     1801 cttcacagga gaaattcagt cctgaattat taccgcgcaa ttttaatatg gctgctgtcg
     1861 gtgtgatgaa gaagggtatg tcgatgagct tattttcttc tctggggcat attgtggttg
     1921 ccggacaatc cggtggcgga aaatctgttc cggtgaaaga actaatcgac agaattattc
     1981 agaaatataa ggaaaccttc gatgaataag ctggtttctg atggcagcgt caaaaaaata
     2041 aattatccgg tgctgtatga gtcaggaata acccctccgt tatgtgaagt cagtgcacca
     2101 gaacctgatg cgggggaaga acgcattgtg gcatatgttt ataaatcatc acggaatacg
     2161 gtttttgaaa atcctgacat tgtaaaaacc tgtacggtca gggatttaaa gaatgatttt
     2221 atgaactgcg atgagaaagg tgagggacaa tgaaacagat ttctttattt attccgctgc
     2281 tggggacgtt attactttcc ggctgtgccg gaacgaactc cgactttgag tgtgatgcca
     2341 ccacatccga tacctgtatg acgatggagc aggccaatga gaaggccaaa aaactggagc
     2401 agtcctcaga agcaaagccg gttgcggcat cactgccgcg cctggctgac gggaacttcc
     2461 ggaccacgcc agtgcaggcc gttgcggcga caacgccgtc ccgcagcagg cctgctgtga
     2521 cagcacttcc ggagcagaaa ctgctggcac cacgcccgct gtttacggct gcccg
//
LOCUS       DAZGXZ010000285         2571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000285 DAZGXZ010000000
VERSION     DAZGXZ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.287"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            247..684
                     /locus_tag="ACHKJF_005400"
     CDS             247..684
                     /locus_tag="ACHKJF_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repA"
                     /protein_id="HFP0952568.1"
                     /translation="MNTQNLIAEHREKHDRDNLGFTQVYPLGWKRLMALAKGNQGAFG
                     LYTFLAQHIDGACGAVVADQAFLADQFDVTTRTVRNWISYLEEQKAIVKIPVAGRVCA
                     YALNPHEVWKGYDNNKPTAAFLTKTLVSKDGEIKRRIMAMFSN"
     gene            1023..1340
                     /locus_tag="ACHKJF_005401"
     CDS             1023..1340
                     /locus_tag="ACHKJF_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V family bacteriocin"
                     /protein_id="HFP0952569.1"
                     /translation="MREITLNEMNNVSGAGDVNWVDVGKTVATNGAGVVGGAFGAGLC
                     GPVCAGVFAVGSSAAVGALYDAAGNSKPTKQKPEGLPPEAWNYAEGRMCNWSPNNLND
                     VCL"
     gene            1360..1572
                     /locus_tag="ACHKJF_005402"
     CDS             1360..1572
                     /locus_tag="ACHKJF_005402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952570.1"
                     /translation="MRPLTENELKNVSGALYESVNTNCVLASGAMIVSAFGGPIGLIS
                     TGLAAIGACATSSSSSSASASAGNGS"
BASE COUNT          738 a          512 c          626 g          695 t
ORIGIN      
        1 aaaaaaacca aaaccaaacg atcgtgcatt aggaaacata gtgctcttaa gataacaatt
       61 agcggaacat gtaagttatt gtttttattt atagaaatgt agcctgatag gaaacacatg
      121 tgtttccgca tgataggaaa cacatgtgtt tcctatcact tctgaaagta ggaatgaaat
      181 aagcattgat gtggaaaata aatttcctac tataggaatg ataatttccg gttgatagga
      241 atcgctatga atacccagaa tctaatcgca gaacatcgag aaaaacatga cagagataac
      301 ctcggattca ctcaagtgta tccactagga tggaagcggc tgatggctct tgccaaagga
      361 aatcagggcg catttggctt gtatactttt ttggcacagc atatcgatgg tgcgtgcggt
      421 gctgttgtag cggatcaggc ttttctggct gatcagtttg atgtgaccac tcgaactgta
      481 cgtaattgga tctcgtacct agaagagcag aaagccatag tcaaaattcc cgttgcggga
      541 agagtttgcg cctatgctct taacccacac gaggtgtgga aaggctatga caataacaag
      601 ccaacagctg cattcttaac aaagacctta gtcagcaaag atggggaaat taagcgccgg
      661 attatggcca tgttttcaaa ttgagccagc ggcaggcaga caatcagggc tacgtgttaa
      721 cgttttgacc atgattgtct gcctgcattt atccaatggt aataaaattg ttacccaaat
      781 catattttta tatatgcaac ttttaatatg aaatgaacga tcgtattgtg aacgttacta
      841 taaattaagc ataggaatat aaatgaaaac gtggcaagta ttcttcatta tattacccat
      901 aagcattatt attagtcttg ttgcgaaaca gctaaatgct agcaaccttg ttcagtctat
      961 agttagcggt atcgctattg catttatgat atctattttt gttaatagga gataagtgac
     1021 gtatgagaga aataacgcta aatgaaatga ataatgtgtc tggtgctggt gatgtcaatt
     1081 gggttgatgt cggaaaaact gtagctacaa acggagctgg tgtggtcggc ggtgcgttcg
     1141 gagcggggct atgcggccct gtttgtgccg gtgtgttcgc tgtcggatct tctgctgctg
     1201 ttggtgcgtt gtatgatgca gcaggaaatt ccaaaccaac gaaacaaaaa ccagaaggac
     1261 tgccaccaga agcatggaac tatgctgaag gtagaatgtg taattggagt ccaaataatc
     1321 ttaatgatgt ttgtttataa tatgaaaagg aattatttaa tgagaccatt aacagaaaat
     1381 gaattgaaaa atgtatctgg tgctttgtat gaaagcgtta atactaactg tgttttagct
     1441 tctggagcaa tgatagtatc tgctttcggt ggacctatag gtttaatatc tactggatta
     1501 gcagccatag gtgcttgcgc cacttcatca tcgtcatctt cggctagtgc ttctgcaggg
     1561 aacggatctt aactattagg agtattcatg ttcgacgaat tgattaagtt agaacttaaa
     1621 tctatatttt tctcgaaaga atacgtgaat atcatagtta caactattat ttctgtaatg
     1681 ttgatgttca ttttatatca gctacctaat aaagactggt taatattctt tacaggaata
     1741 ttaattatat gggagagaat tcttcttttg aaaataagac gaattcagtc agctaaataa
     1801 ctttcacggt tccggcagcg ctttgagcgc aaccggaaac cgtctgggga gaaccccaga
     1861 cccccatcgc tgcaacatcg acggtctacg accacctacc agccgagtgg ctgtctgttc
     1921 cgtgcaaatg ggctttgcag ggggctggcg cggttcctgg cgacctgaaa gcccctggtt
     1981 aggggtatcg gcgcgtctga cctcctgaaa cgctctgtaa gcgtttttcg cgccttccat
     2041 gccctggcgg gcatttaaac cccgtttcgt gcgtctggga gcttctgagc gcgttttaag
     2101 ggccaatatg tacccaacca ttacagacac tagaaaatcg cgttctggtg ctctggatgg
     2161 cttacagaac gcattttcga acagaacagc aacagaatcg ctgacaacat gcgagaggtg
     2221 acaccgctcg ccgcagcccc agcgaccgaa cgtagtgagc gagtgggcga ggaagcggaa
     2281 gagaaccgga agccacatag agcacttacg cactgatgcg gggtgtcggg gtgaagccct
     2341 gaccagatgg ggaatgtgtg agtgcgcgtg cgcggtccga cattcccaca tcctgtcccg
     2401 atttctgagc cgttttagcg gtgaacggac ggcaaaaaca tgatggagct gttgaacgtg
     2461 gagagcacga aatcggacaa aaatcacggc tgacgaggct tgcagaggag taagccagta
     2521 tacactccgc tatcgctgcg tgactggttc agggcttcgc cccgaaaccc c
//
LOCUS       DAZGXZ010000286         2523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000286 DAZGXZ010000000
VERSION     DAZGXZ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.288"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            97..1581
                     /locus_tag="ACHKJF_005403"
     CDS             97..1581
                     /locus_tag="ACHKJF_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0952571.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(1903..2505)
                     /locus_tag="ACHKJF_005404"
     CDS             complement(1903..2505)
                     /locus_tag="ACHKJF_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952572.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
BASE COUNT          656 a          566 c          626 g          675 t
ORIGIN      
        1 caacaaaaat ttttacctca tattgatttg actgctattt ttgctattaa tagccagaat
       61 tgcagtgctt ttatatctgt gtatagtgag gttactatgt cattcggtca gccttgtgat
      121 gagtttcccc tgagttcgct gcctccactc attcgtgatg ccgttattga agctcagcaa
      181 attacgcagg ctccccttgg gcttgtggca gcgtctgccc tgggcgctgt ttcacttgtc
      241 tgccagaatc tgattgatgt ctgcagactg aatacactgc gggggccggt ctctcttttt
      301 ttcctgacgc ttgccgagtc cggagaacga aaaaccgcag tggacaaatt attaatgaag
      361 cccctgtacc agcaggagat gcagttatat tccagataca aaagtgaact ggctgtctgg
      421 aaaaacaagg aagagctttt aaaggctcag aaaaaagcat tgttgtcaaa actgaataaa
      481 gaactccgca aaggcgcgga tgagtcagaa accctcagac aactggaagt acttcagaaa
      541 aacagtgcgg aagaacctgt gaggtataaa ttcattttta atgatgccac gactgcggca
      601 attaagaatc agctttgtgg taaatggcgt tcagtgggta ttatgtcaga tgaagccggt
      661 attatatttg atggatatac cctgtctgag ttgcctttta ttaacaaaat gtgggatggc
      721 tctgtattgt ctgtggacag aaagaatgaa cccgaacaga tgattgaaaa tgccagaatg
      781 acgttatccc tcatggtgca acccggtctt tttgaccgat atatggagcg aaaaggttct
      841 gttgccaggg acagtggttt tcttgcccgt tgtcttatca gtaaacctgc aaccacgcag
      901 gggaaacgct ttattaacgg agctgtgatt ccgggaggat cattaacggc atttcatgaa
      961 cgattaatgg aactggccag aggcagtatt gaaaaatcca gtgaggatga acggtattgt
     1021 ctgcattttt cgcctgaagc gcaaaaaata tttattgagc actataatgt tctggagcag
     1081 gatttatccc cttccggacc cttaagcccg tttcggggac atgtttcaaa aaaaacagaa
     1141 aacattgcaa gaattgcagc gttatttcag tatttcagct atggtgaagg gaaaatctct
     1201 gccgatatca tgacgtctgc tgtcgtaata agttcgtggt acacagatga atataaaaaa
     1261 ttatttgcgt tacctgatga atctgaactg cagcagaagg acgccgagga gctgttcgac
     1321 tggctcattg aggagtgccg cggagaatgc ccgccccgtg tcaggaaaaa ttatattctc
     1381 cagtgtggcc cgggccggtt cagaaacagg aaaaaactga acgcattgct gaacattctt
     1441 gaatctcagt tcaggctttc tgtcgtgccg gaaggaaaaa ccatgtatgt tctgcttccc
     1501 cagatagcca gtttaaagtt gtctgatgtc tctggtattt ttacctcggg ttatcactac
     1561 aataagctga gagcgaagta accggtgagc agtttttctc cattatgctt cagacttaga
     1621 tgattgatta ttatggggat ttcatcaccg atattcagat gtatgaatat cggtgatgaa
     1681 ctaaagctca ggagctgcgt ggtggtgatg aatcaacagg catataactg atacccgccg
     1741 gtcagaagtt ccagcctgcg actttcctgt ctgcgtgccc gcagtccggc gttcaggtgc
     1801 agcgtgtgac agaacgcacg gagagcgaaa cggcaggcag acaggccgtt cagtcagccc
     1861 tggcaccctg cccggatacc agcttccctg aaacaactgg catcaggtgc gcttatcgtc
     1921 aaccgttgtg cggtatgcca ggagcacgta ctgatccatc agatccgtca gtgctgcgct
     1981 ctgcttctga cggttgatga tccattcctc cagctctttt tctgatttcc gtttcagatt
     2041 ctccgtccag tcgtcaaggc agtttccggt cattaaatca agccagcaaa gccagaccag
     2101 tttgaggcgc aactccgtgc caccaaactg acggaagagg ctgatcagtt tatttgccag
     2161 cgaacgctgc ctcagacgtt cacattcaga caggagaatg ccttccagca tcggatgatg
     2221 aaccagcaga tgttcactgg tgcataaatt cactatcaga tcatcatcca ggggcggcgt
     2281 ggtccgtttg tccggaccat aaatcagccg gtgcagactc tggggtacga tggcaatctc
     2341 cggtttttca ccattgaatg acacccaccc gggcgtcaga ttatggaagt agtagatatt
     2401 cggatgacac acgccccagg atacggcgtg ccgggtgaag ccggtcagcc atttctggta
     2461 gctttcgtgg agcagctcgt aacgggtctg atactgtggt ttcattttca tattcctcac
     2521 ggg
//
LOCUS       DAZGXZ010000287         2478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000287 DAZGXZ010000000
VERSION     DAZGXZ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.289"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            47..382
                     /locus_tag="ACHKJF_005405"
     CDS             47..382
                     /locus_tag="ACHKJF_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0952573.1"
                     /translation="MKWIVIDTVIQPSCGISFSAIWSKIKLIIWYQSDVFLPPESIFT
                     LTHTGIVLNNKVLPVTIYNVVPFNKRFWDLIKNSQECPANSDNVLKECFNNHCILQIC
                     PYGLKQQCP"
     gene            complement(684..848)
                     /locus_tag="ACHKJF_005406"
     CDS             complement(684..848)
                     /locus_tag="ACHKJF_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0952574.1"
                     /translation="MTVKLRLAAVALLLFLVVMVDFTSRIMSVLADGVLVVGIVVVLF
                     PLVKKDMPGS"
     gene            complement(845..1276)
                     /locus_tag="ACHKJF_005407"
     CDS             complement(845..1276)
                     /locus_tag="ACHKJF_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance protein"
                     /protein_id="HFP0952575.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATITGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
     gene            complement(1446..1522)
                     /locus_tag="ACHKJF_005408"
     tRNA            complement(1446..1522)
                     /locus_tag="ACHKJF_005408"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1486..1488),aa:Arg,seq:tct)
     gene            complement(1536..1612)
                     /locus_tag="ACHKJF_005409"
     tRNA            complement(1536..1612)
                     /locus_tag="ACHKJF_005409"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1576..1578),aa:Arg,seq:tcg)
     gene            complement(1622..1697)
                     /locus_tag="ACHKJF_005410"
     tRNA            complement(1622..1697)
                     /locus_tag="ACHKJF_005410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1662..1664),aa:Ile,seq:cat)
     gene            complement(1738..>2478)
                     /locus_tag="ACHKJF_005411"
     CDS             complement(1738..>2478)
                     /locus_tag="ACHKJF_005411"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0952576.1"
                     /translation="KPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAK
                     GRALKQHVMAPLIAYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYL
                     KLQALFARVAEEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVP
                     YTDVWTHKPVQYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALG
                     RRATGVELETERFEQTVREVQDLASQNG"
BASE COUNT          625 a          709 c          533 g          611 t
ORIGIN      
        1 tatacttttc agaacatcac cagcacagca taaataagga ggctaaatga agtggattgt
       61 gattgataca gttatccagc catcatgcgg aatatctttt tcagccatat ggagtaaaat
      121 aaaattaata atctggtatc aatcggatgt tttcttacct cctgaaagta tatttacgtt
      181 gactcacaca ggtatcgtac ttaataacaa agtgctacct gtaaccattt acaacgttgt
      241 accattcaat aaaagattct gggatttaat caaaaacagc caggaatgtc ctgcaaattc
      301 agataacgta ttgaaagaat gctttaataa tcattgcatt ctgcaaatat gcccttacgg
      361 actaaaacaa cagtgtccat aattagttta ctcatatctt acaaaattca tataaaagcc
      421 cctccggaga ggggctaaag ccgcgtatct gtatcatcat gcgcatgatg ccgggtgcct
      481 cccggtgagt tcagcccggt gccaccaaac ccgcgcgttt actttcctgg gtatcaaaga
      541 taacacctaa tgcaccagtc gcccctccgc tcagggggat tcaccatgcc tgtttctttt
      601 aacaaactcc ccgcaaaaca gacaactgtc aaccgtctga attgtgagac atttaaaatt
      661 ttcggggcat gactgatacc cggctaacta cctggcatgt cctttttcac caaaggaaaa
      721 agcaccacca caataccgac caccagcact ccatccgcca gcaccgacat gatcctgctg
      781 gtgaaatcca ccatcaccac cagaaacagc aggagtgcca cagcggccag acgcagtttt
      841 accgtcacag gtggttctcc agtcgcaggc caagaacacc agcaatctct tccagaacct
      901 tacgctcttc cggctcaatt tcaccatctg cttcagcaat ggccaccgcc acatccagca
      961 catcttccgc ttcacgcgta tcgtgtttca catcttcaat ttcacgcaac gccgctcgac
     1021 gaccaatttt aaagttggtg tcaagctgac ctgtgatcgt ggcactgata gcattaattt
     1081 ctgacgtaaa cgcggacaac gctggctggt tacgcaagac ctgctcgatc ttcgctttct
     1141 ctgaagcctc acattcacca tctgcatagg ctaccaggta ggcagcatta ataaccgcct
     1201 gtgccagatc acgtttctca aactttttaa tttccgctgc tgctctgcgg gttttctttt
     1261 tgaaaatacc aaacatcgtg acgttccttt aggtgggtga gccaacgccc gggagcgatc
     1321 tgcccacaga gaaagtcaca ctgaccactc cgtaagctca cccccgaaag gctctgtggt
     1381 tgatatgcgc cgggcgtggc gcggatacaa aaaaggcccg caaaagcgag cctggtaaat
     1441 aaatatggcg cgttgtactg gattcgaacc agtgaccgat tgcttagaag gcaattgctc
     1501 tgtccggctg agctaacaac gctgaatacc gataatggac cgccatcgag gactcgaacc
     1561 ccgcgcaacc agcttcgaag gctgacgctc tatcccgatg agctaatggc ggtatgtgat
     1621 atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc tcgctctcac
     1681 cactgagcta aagggccgag tgcaggataa taacggtacg taactaatac tgcaatatca
     1741 tccgttctga ctggctaaat cctgaacttc cctgaccgtc tgctcaaaac gttcagtctc
     1801 cagctcaacg ccagttgcac gacgccccag cgccatcgcg gctttgactg tcgaacccga
     1861 ccccatgaaa aaatctgcaa ccaggtcccc cggacgactg ctcgcgctga ttatctgctg
     1921 cagcatttct gccggttttt cgcacggatg tttcccggga tagtactgca ccggtttatg
     1981 cgtccacaca tcggtgtacg gcacctgcgc cgtcacgcca aaataccgcc gcagatgctt
     2041 atattcactc tgcagttccg catactgccg gttcagtgaa gtatacgtct ccagcagctg
     2101 gtggtggggc ttctccagtt caccccgctg atgcttctct tctgccaccc gggcaaacag
     2161 cgcctgtaat ttcagataat cactttcgtt cggcagttgc cactgactgg cactgaacca
     2221 gtgcgacacc atgtttttct ttcctgtggc atccactatc tgttttgccg ttatccccag
     2281 ggcagcacgc gcatcacgaa agtaagcaat cagcggagcc atcacatgct gtttcagtgc
     2341 cctgcccttc gcctcatacc cggcatcttt cggacgatac ggcccctgat aatgttccgc
     2401 gaacagaatg cgctctgtgg cggggaaata cgccctcagg ctttccttgt tgcacccgtt
     2461 ccagcgtccg gacggctt
//
LOCUS       DAZGXZ010000288         2454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000288 DAZGXZ010000000
VERSION     DAZGXZ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.290"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..2050
                     /locus_tag="ACHKJF_005412"
     CDS             <1..2050
                     /locus_tag="ACHKJF_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001340207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0952577.1"
                     /translation="KSRKSVKSVEPDIADRANNWLKSYGLDYKLEQAPLNNEIDNALD
                     AYFSKNGGTGGNRPDVKLLVQDKYGKQYPVLIEYKGYKDRLIRLGSNGIIENKDARKE
                     WNFKNINGYAVNGAVHYANALLQFTNYPDIIAIGMTGWRDDGTGELHHEIGVWYVSKN
                     NLGAGQKVGEFTDLSFLADKNVDGFLNKIKLLNLPPEELEKIKASKEEEIDTRLSRLN
                     NDIYQNEKGLGESDRVYLVVATVIATLGIPGKLAPLDKKELTSSTEEDLRDGDIIFRK
                     IRNFLRLKAVPETKREMILRSLQNTLWTENINKPVNGESQLKRVFVKVVDDLGEYYKI
                     GLTTDFTGKLFNEMYRWLGFTQDKLNDVVLTPPYVATLLARLARVNKDSYVWDFATGS
                     AGLLVAAMNEMLIDARENIHSPNELQLKEAQIKAEQLLGLEVLSSIYMLAILNMILMG
                     DGSSNILNKDSLADFDGKYGFGKTGEKFPADAFILNPPYSAKGNGMIFVQKALSMMDK
                     GYAAVIIQSSAGTGKATEYNKKILKENTLLASIKMPADLFIGKSSVQTYIYVFQVKIP
                     HNAKQAVKFIDFSNDGYARSNRKKARNNLVDADRAKERYQEVVDLVHFGKGCLNIFTE
                     DEYFEGTIDPDSGEDWNQTRPVDARPTLDDFKKTVGDYLAWEVSQLLKKQGENNFAGK
                     "
     gene            2092..2454
                     /locus_tag="ACHKJF_005413"
                     /pseudo
     CDS             2092..2454
                     /locus_tag="ACHKJF_005413"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          758 a          455 c          590 g          651 t
ORIGIN      
        1 taaatcaaga aagtctgtaa aatctgttga accagacatt gccgaccggg ctaacaactg
       61 gctcaaatct tatggacttg attacaaact ggagcaggca ccacttaaca acgagatcga
      121 taacgcactg gatgcttatt tttcgaaaaa tggcggcaca ggcggtaatc gtcccgacgt
      181 aaaactcctt gttcaggaca aatacggcaa gcagtatccc gttctcatcg agtataaagg
      241 ctacaaggac aggcttatca gacttggcag caacggtatt attgagaaca aagatgccag
      301 aaaagaatgg aattttaaaa atattaatgg atacgcggtg aatggcgcag tccattatgc
      361 caacgcactt cttcagttca ccaattaccc tgatattatt gctattggta tgaccggctg
      421 gcgcgatgat ggtacaggcg aactgcatca tgaaatcggc gtgtggtacg tatcaaagaa
      481 taatttagga gccgggcaga aggttggtga attcaccgac ttatcattcc ttgctgacaa
      541 aaatgttgat ggatttctca acaagataaa gctgcttaat cttcctcccg aagaattgga
      601 aaaaatcaag gccagcaaag aggaagagat agacacgcga ctttcccgtc tgaataacga
      661 tatttaccag aatgaaaagg ggcttggtga aagcgatcgt gtttaccttg ttgtggctac
      721 cgttattgca acgttgggga ttccgggaaa gttagcaccg ctggataaaa aagaactgac
      781 ctcttcaacg gaggaggact tgcgtgatgg tgatattatt ttcaggaaaa taaggaattt
      841 tttaaggctg aaagctgttc ctgaaacaaa aagagaaatg attttgcgct cgttgcaaaa
      901 tacgctgtgg actgaaaaca ttaacaaacc agtcaacggt gaaagccagt taaagcgcgt
      961 gtttgttaaa gttgttgatg atcttgggga gtattacaaa atcggactga ccactgattt
     1021 tacgggcaaa ctgtttaatg agatgtatcg ctggcttgga tttacgcagg acaaactcaa
     1081 tgacgtcgtt ctgacaccac cttatgttgc cacattgctg gcacgactcg ccagagtaaa
     1141 caaagattcc tatgtgtggg attttgctac aggttctgcg ggattgcttg tggctgcaat
     1201 gaatgaaatg ctcattgacg ccagagaaaa tattcactca ccgaatgaac tacaacttaa
     1261 ggaagcgcaa atcaaggctg aacagcttct tggccttgag gtgttatcca gtatttatat
     1321 gctggccatc ctgaatatga ttttgatggg ggatggtagc tcgaatatcc tcaataaaga
     1381 ctcgctagcc gattttgatg gcaagtatgg atttgggaaa acaggagaaa aattccctgc
     1441 ggacgcattt attctcaatc ctccgtattc agctaaaggt aacggcatga ttttcgtgca
     1501 gaaggcgctg tcgatgatgg ataaaggcta tgctgcagtg attatccaga gttcagccgg
     1561 cacaggaaag gctacggagt acaacaaaaa aatactcaag gaaaatacct tgctggcaag
     1621 catcaaaatg cctgcggatc tgtttattgg taaatcaagc gttcagacgt acatttacgt
     1681 ttttcaggta aaaattccgc ataacgcgaa acaggctgtt aagtttatcg acttttccaa
     1741 tgacggctat gctcgctcta atcgcaaaaa ggcaagaaac aatcttgttg atgccgatcg
     1801 tgcaaaagag cgttatcagg aggttgtgga tctggttcac ttcgggaaag gttgccttaa
     1861 tatctttacg gaggatgaat attttgaggg aacaatcgat cctgatagtg gtgaggactg
     1921 gaatcagacc aggccagtag atgctaggcc aacgctggat gatttcaaaa agacggtagg
     1981 tgattacctt gcatgggagg tctcgcaatt actgaaaaaa caaggtgaaa acaattttgc
     2041 gggaaagtaa attccccgct cagcgataaa ttaagaagcg ttgagtgggg agagttcagg
     2101 attggcgatt tgttctatgt gagcacaggc tcgcttatct cctcaggaag attaaaacca
     2161 ggcaaaatta aacgtgtttc agcaaagagc gatcaaaacg gtatcatcgg agagtttgat
     2221 accgagttta tggatgatgc aaggcattat gaaaatttta taagcgttaa tttttttgga
     2281 gatgcttttt atcatcctta ttgtgccagc gtagaaatga aggtgcatgt tcttacatta
     2341 aaatcaagta agtccgactt taatgcaaag cgcggactgt atgttgccag tatgattaat
     2401 aaagctttaa aggggcgttt taattatggt caacagttat cgagttccaa actc
//
LOCUS       DAZGXZ010000289         2427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000289 DAZGXZ010000000
VERSION     DAZGXZ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.291"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..572
                     /gene="eco"
                     /locus_tag="ACHKJF_005414"
     CDS             63..572
                     /gene="eco"
                     /locus_tag="ACHKJF_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP0952578.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(721..2367)
                     /gene="mqo"
                     /locus_tag="ACHKJF_005415"
     CDS             complement(721..2367)
                     /gene="mqo"
                     /locus_tag="ACHKJF_005415"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP0952579.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          571 a          667 c          609 g          580 t
ORIGIN      
        1 gcaaattaat aaatctgatg aatatgttaa ccttcagcga catcatcggt gaaaacctat
       61 aaatgaagaa ggaaagcaaa aaaatgaaga ccattctacc tgcagtattg tttgccgctt
      121 tcgctaccac ttccgcctgg gcggcagaaa gcgtccagcc actggaaaaa atcgcgcctt
      181 atccacaagc tgaaaaaggg atgaagcgtc aggtgattca gttaaccccg caagaagatg
      241 aatctaccct gaaagtagaa ctgttaatcg gtcagacgct ggaagtcgat tgcaatttgc
      301 atcgtctcgg cgggaagctg gaaagcaaaa cgctggaagg ctggggctat gattattatg
      361 tctttgataa agttagctcg ccggtttcca cgatgatggc ctgcccggat ggcaagaaag
      421 agaagaaatt tgtcaccgcg tatctgggcg atgctggaat gctgcgttac aacagcaagc
      481 tgccgatcgt ggtgtatacg ccagacaatg tagatgtgaa gtaccgcgtc tggaaggcgg
      541 aagagaaaat tgacaacgcg gtagttcgct aaaatgctgt gaagtgcggc accccgtagg
      601 ccgaataagg cggtcacgcc gcatccgaca tctaacgccc gagccggtgc ctgatgcgac
      661 gctggcgcgt cttatcagca tacgccacat ccggcatacc atgccggatg tggcacatca
      721 ttacaacgca atatccgcca cttctttttg aacgggttgc ggtttcaact gcggttttgg
      781 cgtactatct gctgcctgcg gcttgtcgta tttcagcccc agcacttcgc tggtgtactg
      841 caactcgcgt tctgttgccg ctacatcacc gttcagcttg cgtccataag acggaacgat
      901 cgctttcaac gtagcctgcc attgcgggct ggaaacgcga tcgccaaata ctttttccag
      961 cagatccaac ataatcggcg cggcggttga cgcccctggc gatgccccca gcagcgcggc
     1021 aatggtccct tgctggtcac tgaccacttc agtacccaga cgcagtacgc cacctttctc
     1081 ggcatcacgc ttgataatct gcacgcgctg ccccgcttgc cacaaacgcc agtcctcttt
     1141 ttttgcctgc ggatagtact ctttcaacgc ttcaaaacga tcctcttcac tcaacatcac
     1201 ctgactcacc agatatttca ccagatcgaa attatccagc ccgacgtgca tcatcggcat
     1261 cacgttagag gtggtggtgg aactcatcag atcccacaat gaaccgtttt tgaggaattt
     1321 ggttgagaag gtggcaaatg gcccaaacag cactacgcgt ttaccgtcca gaacgcgggt
     1381 atcgatatgc ggaaccgaca tcggtggtgc gccaacggat gctttaccgt aaaccttcgc
     1441 cagatggtga ttaaccacgt ccgggttttc cgaaacaagg aactgtccgc ccaccgggaa
     1501 accggcgtag tctttcgctt ccggaatccc cgattcctgt aatagcttca gcgccgcacc
     1561 gcccgcgccg ataaagacaa atttggcgcg gatgttctgt gcagtgccgt ttttcagatc
     1621 ggcaacggta acggtccagg tgttatcgtc attacgcttt agggcgcgga cttcgctgct
     1681 gagttgcagc gagaagttag atttcttctg taaggaagca attaactggc gggtgatctc
     1741 gccatagttc acatcagtgc caatttctgt gcgtgttgcc gctactttct gttgtggatc
     1801 gcgcccttcc atcactaacg gtgcccactc tttgatctgc gcgtgatctt cagagtaacg
     1861 cataccgcga aacagcgagc tttgttgcaa cgcggcgtaa cgggcgcgca ggaaattaac
     1921 gttatcctcg ccccagacaa agctcatatg cggaacggta ttgataaatg aacgcggagt
     1981 acgcagcacg ccgcgctcaa cctggtgcgc ccagaactgg cgggaaatct gaaatgcttc
     2041 gttaatggcg actgcttttt caatactgat gctgccatcg gcgttttgcg gggtgtagtt
     2101 cagttccatc agtgcagaat gcccggttcc ggcgttattc cagccgttcg aactctcctg
     2161 cgcgacaccc tccaggcgct ccaccatggt catcgaccat tcaggctcca gctcgcgtaa
     2221 ataggtcccc aacgtggcgc tcataatgcc gccgccaatc aacagtacat cagtttcctg
     2281 ctcctcggac gctttcgctt ttgccgccat cgaaacggca ttaagcccca cggccatcga
     2341 gaagagcatg gcagtcactt ttttcatctt gttaatgcct tacttttagt cgctttattg
     2401 caggtgagat ttgcgcggca tcaacgg
//
LOCUS       DAZGXZ010000290         2400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000290 DAZGXZ010000000
VERSION     DAZGXZ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.292"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(205..414)
                     /gene="fumD"
                     /locus_tag="ACHKJF_005416"
     CDS             complement(205..414)
                     /gene="fumD"
                     /locus_tag="ACHKJF_005416"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP0952580.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            769..963
                     /locus_tag="ACHKJF_005417"
     CDS             769..963
                     /locus_tag="ACHKJF_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952581.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            971..2383
                     /gene="pykF"
                     /locus_tag="ACHKJF_005418"
     CDS             971..2383
                     /gene="pykF"
                     /locus_tag="ACHKJF_005418"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP0952582.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKIVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          675 a          572 c          512 g          641 t
ORIGIN      
        1 tatttattat catttgaaat aaatttaact taagcgacaa ttatttgtga cgttcattgc
       61 agaaaataaa aataatatta tcgttataaa ttaaaataca taccgcgatt cgtataattt
      121 cagcagttgt tatttagaat gattaactgg accgcaactg aagcaagaat atcaggatga
      181 agcttaccgc ctcatcctga atggtcattt taccagtgcg ttaatgacgg tttccatcgc
      241 ctgcttttcc agctcactgc gctgaatgcg tttattggct aatgctgtac gtaatacgcc
      301 tgcgataaca atatgctttg gttcctgccc cagatcgcgc atttccagca caactttacc
      361 gacaactctg cacatttctc ggtacagttc atcctctttt gtccgatttc ccattcttta
      421 tacctattta tcatgccaac tatcagcata tatcaatcta accaattaaa caaaaatcaa
      481 acaaaatcag acaaataacg cgataaatta ttttaactgt agcaattgag cgatgatgta
      541 tttatacacc ggatgaactt tcacttatcc tcacactgac aacttcggca ccagacgttg
      601 cgcaaacagt gaagtttttg cgtaaccttt tccctggaac gttaaatctt tgataacaat
      661 ttattgtcta acaagttgta tattttttga aacgctgttt ttgttttcct tttggattaa
      721 tttcagcgta taatgcgcgc caattgactc ttgaatggtt tcagcacttt ggactgtaga
      781 actcaacgac tcaaaaacag gcactcacgt tgggctgaga cacaagcaca cattcctctg
      841 cacgcttttt cgatgtcacc tatccttaga gcgaggcacc accactttcg taataccgga
      901 ttcgctttcc ggcagtgcgc ccagaaagca agtttctccc atccttctca acttaaagac
      961 taagactgtc atgaaaaaga ccaaaattgt ttgcaccatc ggaccgaaaa ccgaatctga
     1021 agagatgtta gctaaaatgc tggacgctgg catgaacgtt atgcgtctga acttctctca
     1081 tggtgactat gcagaacacg gtcagcgcat tcagaatctg cgcaacgtga tgagcaaaac
     1141 tggtaaaacc gccgctatcc tgcttgatac caaaggtccg gaaatccgca ccatgaaact
     1201 ggaaggcggt aacgacgttt ctctgaaagc tggtcagacc tttactttca ccactgataa
     1261 atctgttatc ggcaacagcg aaatggttgc ggtaacgtat gaaggtttca ctactgacct
     1321 gtcagttggc aacaccgtac tggttgacga tggtctgatc ggtatggaag ttaccgccat
     1381 tgaaggtaac aaagttatct gtaaagtgct gaacaacggt gacctgggcg aaaacaaagg
     1441 tgtgaacctg cctggcgttt ccattgctct gccagcactg gctgaaaaag acaaacagga
     1501 cctgatcttt ggttgcgaac aaggcgtaga ctttgttgct gcttccttta ttcgtaagcg
     1561 ttctgacgtt atcgaaatcc gtgagcacct gaaagcgcac ggcggcgaaa acatccacat
     1621 catctccaaa atcgaaaacc aggaaggcct caacaacttc gacgaaatcc tcgaagcctc
     1681 tgacggcatc atggttgcgc gtggcgacct aggtgtagaa atcccggtag aagaagttat
     1741 cttcgcccag aagatgatga tcgaaaaatg tatccgtgca cgtaaaatcg ttatcactgc
     1801 gacccagatg ctggattcca tgatcaaaaa cccacgcccg actcgcgcag aagccggtga
     1861 cgttgcaaac gccatcctcg acggtactga tgcagtgatg ctgtctggtg aatccgcaaa
     1921 aggtaaatac ccgctggaag cggtttctat catggcgacc atttgcgaac gtaccgaccg
     1981 cgtgatgaac agccgtctcg agttcaacaa tgacaaccgt aaactgcgca ttaccgaagc
     2041 ggtatgccgt ggtgccgttg aaactgctga aaaactggat gctccgctga tcgtggttgc
     2101 tactcagggc ggtaaatctg ctcgcgcagt acgtaaatac ttcccggatg ccaccatcct
     2161 ggcactgacc accaacgaaa aaacggctca tcagttggta ctgagcaaag gcgttgtgcc
     2221 gcagctggtt aaagagatca cttctactga tgatttctac cgtctgggta aagaactggc
     2281 tctgcagagc ggtctggcac acaaaggtga cgttgtagtt atggtttctg gtgcactggt
     2341 accgagcggc actactaaca ccgcatctgt tcacgtcctg taatattgct tttgtgaatt
//
LOCUS       DAZGXZ010000291         2400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000291 DAZGXZ010000000
VERSION     DAZGXZ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.293"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            72..452
                     /locus_tag="ACHKJF_005419"
     CDS             72..452
                     /locus_tag="ACHKJF_005419"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0952583.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEAPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            449..796
                     /gene="tnpB"
                     /locus_tag="ACHKJF_005420"
     CDS             449..796
                     /gene="tnpB"
                     /locus_tag="ACHKJF_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0952584.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLPSLTML"
     gene            846..2384
                     /locus_tag="ACHKJF_005421"
     CDS             846..2384
                     /locus_tag="ACHKJF_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP0952585.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKGIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIAENALRAVSLGRKN
                     FLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIA
                     LPAE"
BASE COUNT          555 a          665 c          696 g          484 t
ORIGIN      
        1 tctggtcaga atctgacgaa ttagacaaag tggtgtccac caaataagta gtgggaacca
       61 aagtatcaga tatgcagaaa aatgtgactc ccggcaggcg aaaaggctgc cctaattatc
      121 ctcccgaatt taaacagcag ctcgttgctg cctcctgtga acccgggata tccatctcaa
      181 aacttgctct tgaaaatggc attaacgcca atctgttgtt caaatggcga caacaatggc
      241 gcgagggaaa actgctatta ccttcttcag agagccccca gctacttcct gtgactctcg
      301 atgccgctgc cgaacagcca gaatcgctcg cagaggcccc ggaaaccctc agtatcagct
      361 gtgaggtaac gttccggcac gggacgctcc gcttcaatgg caatgtcagc gaaaagctcc
      421 tgactctgct gatacaggaa ctgaagcgat gatcccgtta ccttccggga ccaaaatttg
      481 gctggttgcc ggtatcaccg atatgagaaa tggcttcaac ggcctggctg cgaaagtaca
      541 aacggcgctg aaagacgatc ccatgtccgg ccatgttttc attttccggg gccgcagcgg
      601 cagtcaggtt aaactgctgt ggtccaccgg tgacggactg tgcctcctga ccaaacggct
      661 ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc aaagtgttcc ttacgcaggc
      721 gcagctggcg atgctgctgg aaggtatcga ctggcgacag cctaagcggc tgctgccctc
      781 cctgaccatg ctgtaaatct ctttatcctg gttgtcacag aataagcccg gtaaaatacg
      841 ggcttatgaa cgacatctct tctgacgaca tcttcctgct gaaacagcgc ctggccgaac
      901 aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa ccgggagcgc gaaatagacc
      961 atctgcaggc gcagttggat aaactccgcc ggatgaactt cggcagtcgt tccgaaaaag
     1021 tctcccgccg tatcgcacaa atggaagccg atctgaaccg gcttcagaaa gagagcgata
     1081 cgctgactgg tagggtgtat gacccggcag tacagcgtcc gttgcgtcag acccgcaccc
     1141 gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg actgttgcct gcggcgccgt
     1201 gctgcccgaa ctgcggcggt tcactgagct atctgggcga ggataccgcc gaacagctgg
     1261 agttgatgcg tagcgccttc cgggttatcc ggacggtacg ggaaaaacat gcctgtactc
     1321 agtgcgatgc catcgtgcag gcacctgcac cttcgcggcc catcgagcgg ggtatcgccg
     1381 gaccggggct gctggcccgc gtgctgacct cgaagtatgc agagcacacc ccgctgtatc
     1441 gccagtcaga aatatacggc cggcaaggtg tggagctgag gcgttcactg ctgtcgggct
     1501 gggtggatgc atgctgccgg ctgctgtctc cgctggaaga ggcgcttcat ggctatgtca
     1561 tgactgacgg caaactccat gccgatgata ccccggtcca ggtactgctg ccgggtaata
     1621 agaagacgaa gaccgggcgg ttgtgggcgt atgttcgtga tgaccgcaat gccgggtcag
     1681 cgttggcacc tgcagtgtgg ttcgcttaca gcccggacag aaaaggcatc catccgcaga
     1741 ctcatcttgc ctgcttcagc ggtgtgctgc aagcggatgc gtacgccggg ttcaacgagc
     1801 tttatcgcaa tggtgggata acggaagctg cctgctgggc tcatgcccgc cgaaagatcc
     1861 acgatgtgca cgtccgcatc ccgtcagcac tgacggaaga agccctggag cagatcggtc
     1921 agttgtacgc catagaggcg gatataaggg gaatgccggc agagcagcgg cttgctgaac
     1981 gtcagcgaaa aacgaaaccg ttgttgaaat ccctggaaag ctggttgcgt gaaaagatga
     2041 agaccctgtc gcgacactca gagttggcga aggcgttcgc gtacgcactt aaccagtggc
     2101 cggcactgac gtactatgcg aacgatggct gggtggaaat cgacaacaac atcgctgaaa
     2161 atgccctgcg ggcggtcagt ctgggtcgta aaaacttcct gttcttcggc tctgaccatg
     2221 gtggtgagcg gggagcgcta ctgtacagcc tgatcgggac gtgcaaactg aatgacgtgg
     2281 atccagaaag ctaccttcgc catgtgcttg gcgtcatagc agactggccg gtcaaccggg
     2341 tcagcgaact gcttccgtgg cgcatagcac tgccagctga ataacacatc cccgtcaata
//
LOCUS       DAZGXZ010000292         2359 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000292 DAZGXZ010000000
VERSION     DAZGXZ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.294"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..904
                     /locus_tag="ACHKJF_005422"
     CDS             <1..904
                     /locus_tag="ACHKJF_005422"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HFP0952586.1"
                     /translation="QFIEKPNHSTAEEYVSTGEYLWNSGMFMFKASVYLEELKKHRPD
                     IYEICEQTASLSYNDLDFIRLPKHIFQDCPSESIDFAVMEKTKNCIVRPVDIGWSDVG
                     SWHSLWDISDKTQDGDVCKGDILTYNTKNNYIYSESALVAAVGVEDIVIVQTKDAILV
                     SKKTEVQDVKKIVEMLKEHERSEYISHREEFRPWGKFDAIDQGERYKVKKIVVKPGEG
                     LSLRMHHHRSEHWIVLSGTAKVTLNDKTVLLAANESIYIPLGATYSLENPGIIPLNLI
                     EVSSGDYLGEDDIVRQKERYRIDD"
     gene            908..2287
                     /gene="cpsG"
                     /locus_tag="ACHKJF_005423"
                     /gene_synonym="manB"
     CDS             908..2287
                     /gene="cpsG"
                     /locus_tag="ACHKJF_005423"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0952587.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQITLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDVIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLADPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGNIALMNEQVEFI
                     TKFLNGDGL"
BASE COUNT          687 a          510 c          584 g          578 t
ORIGIN      
        1 acaatttatt gaaaaaccta accattcgac ggcagaagag tatgtttcca caggagaata
       61 tctctggaat agtggaatgt ttatgtttaa agcatcagta tatttagagg aattgaaaaa
      121 gcacaggcct gatatatacg aaatctgtga gcagacagct tcattatcct ataacgatct
      181 tgatttcata cgattgccta aacatatttt ccaagattgt ccgtcggaat caattgattt
      241 tgcggttatg gaaaaaacaa aaaattgcat tgttcgtcca gtagacatcg ggtggagtga
      301 tgtaggctct tggcattctt tgtgggatat tagtgataaa acgcaggatg gagatgtctg
      361 taaaggtgat attctaactt ataacactaa aaacaattat atatattctg aatcagccct
      421 agtggctgct gtaggtgtag aagatatagt tattgttcaa acaaaagatg ctattctcgt
      481 gtccaaaaaa actgaggttc aggatgtaaa aaaaatagta gaaatgctta aggaacatga
      541 gcgctcagag tatatatcgc atcgagagga gtttcggcct tggggaaaat ttgatgcgat
      601 agatcaggga gaacgatata aagttaaaaa gatagttgtg aaacccggcg aaggactatc
      661 cttacgaatg caccatcatc gttctgagca ttggattgtt ctttccggca cagcaaaagt
      721 aactttaaac gataaaacag tattgcttgc tgcaaatgaa tcaatctaca ttcctctagg
      781 agcaacgtat agtcttgaaa atccaggaat aatccctctg aatcttattg aggttagttc
      841 aggcgattat ttaggtgagg atgatattgt acggcaaaaa gaacgctaca gaatagatga
      901 ctaatatatg aaaaaattaa cctgctttaa agcctatgat attcgcggga aattaggcga
      961 agaactgaat gaagatatcg cctggcgcat tggtcgcgcc tatggcgaat ttctcaaacc
     1021 gaaaaccatt gtattaggcg gtgatgtccg cctcaccagc gaaaccttaa aactggcgct
     1081 ggcaaaaggt ttacaggatg cgggcgtcga tgtgctggat attggcatgt ccggcaccga
     1141 agagatctat ttcgccacgt tccatctcgg cgtggatggc ggcattgaag ttaccgccag
     1201 ccataacccg atggattata acggcatgaa actggtgcgc gaaggggctc gcccgatcag
     1261 cggtgatacc ggactgcgcg acgtccagcg tctggcagaa gccaacgact tcccccccgt
     1321 cgatgaaacc aaacgcggtc gctatcagca aattaccctg cgtgacgctt acgttgatca
     1381 cctgttcggt tatatcaatg tcaaaaacct cacgccgctc aagctggtga tcaactccgg
     1441 gaacggcgca gcgggtccgg tggtggacgt tatcgaagcc cgctttaaag ccctcggcgc
     1501 accggtggaa ttgatcaaag tgcacaacac gccggacggt aatttcccca acggtattcc
     1561 taacccgcta ctgccagaat gccgcgacga cacccgcaat gcggtcatca aacacggcgc
     1621 ggatatgggc attgcctttg acggtgattt tgatcgctgc ttcctgtttg acgaaaaagg
     1681 gcagtttatt gagggctact acattgtcgg cctgctggca gaagcattcc tcgaaaaaaa
     1741 tcccggcgcg aagatcatcc acgatccacg tctctcctgg aacaccgttg atgtggtgac
     1801 cgccgcgggc ggcactccgg tgatgtcgaa aaccggacac gcctttatta aagaacgtat
     1861 gcgcaaggaa gacgccatct acggtggcga aatgagcgcc caccattact tccgtgattt
     1921 cgcttactgc gacagcggca tgatcccatg gctgctggtc gccgaactgg tatgcctgaa
     1981 aggaaaaacg ctgggcgaac tggtgcgcga ccggatggca gcatttccgg caagcggtga
     2041 gatcaacagc aaactggcgg accccgttga ggcgattaac cgcgtggaac agcattttag
     2101 ccgtgaggcg ctggcggtgg atcgcaccga tggcatcagt atgacctttg ccgactggcg
     2161 ctttaacctg cgctcctcca acaccgaacc ggtggtgcgc ctgaatgtgg aatcgcgcgg
     2221 taatatagca ttaatgaatg aacaggttga atttattaca aaatttttaa acggtgatgg
     2281 tttatgagaa tattaaaaat tattataagt gtattgttgt ttataaaaaa gaaaagaaaa
     2341 aaaataccgt actcagcaa
//
LOCUS       DAZGXZ010000293         2298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000293 DAZGXZ010000000
VERSION     DAZGXZ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.295"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(279..401)
                     /locus_tag="ACHKJF_005424"
     CDS             complement(279..401)
                     /locus_tag="ACHKJF_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004118124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0952588.1"
                     /translation="MVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK"
     gene            complement(346..498)
                     /locus_tag="ACHKJF_005425"
                     /pseudo
     CDS             complement(346..498)
                     /locus_tag="ACHKJF_005425"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5431 family protein"
     gene            complement(648..962)
                     /locus_tag="ACHKJF_005426"
     CDS             complement(648..962)
                     /locus_tag="ACHKJF_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="theronine dehydrogenase"
                     /protein_id="HFP0952589.1"
                     /translation="MKWRYSLRWKLPYPCPGEHELVSEVVEAGQPAPASVMSRWVAGA
                     GYAVCLDFISDRPVRRWSEERKAAVRRRNLEKRINRHAPLFADELIARELAERPDYFQ
                     GK"
     gene            complement(959..1678)
                     /locus_tag="ACHKJF_005427"
     CDS             complement(959..1678)
                     /locus_tag="ACHKJF_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP0952590.1"
                     /translation="MSVRSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRWISLSDLRFFMPSLTAEELHGNRLQWLYAIDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERRRHKSALVMQKYSRQQAREAEQKTRAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEAGMAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            complement(1675..2109)
                     /gene="psiB"
                     /locus_tag="ACHKJF_005428"
     CDS             complement(1675..2109)
                     /gene="psiB"
                     /locus_tag="ACHKJF_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0952591.1"
                     /translation="MKTELTLDVLQTMSAQEYEDIRAAGSGERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFVPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            complement(2164..>2298)
                     /locus_tag="ACHKJF_005429"
     CDS             complement(2164..>2298)
                     /locus_tag="ACHKJF_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HFP0952592.1"
                     /translation="MKKGDAAEHAEFHMKDNRWVPGWMCTPRPQMDATEHTTNLADAA
                     "
BASE COUNT          495 a          724 c          600 g          479 t
ORIGIN      
        1 ggaagaaaaa gagaataatc acgggaaagg taatgtcatt tctgacaact ggccataaac
       61 agagaaggcg ggggaaaaga acgagcgccg caggcgcacg ttcattcccg ttcccggagg
      121 gaagtggcct ttctggcagt ctggttgttc atgctgctac aatgccagca aggagaaagg
      181 ggccaccggc gaaccagcag cccctttata aaggcgcttc agtgcagtca gaccagcatc
      241 agccctgaaa aggcgggcct gcgcccgcct ccaggttgct acttaccgcc ggattcgtaa
      301 gccattgttg ccgtaacttc cctgtatccg tccttcagcc ggacttcgca caggcgattc
      361 cgggtcagga gtgtgaatat caacaacgtg cagcacacga ttaatacgca ccagataagg
      421 gcgtttcccg gtagtttcat ggtttactcc tccttgcaat ttgcacggta agaggccata
      481 ctgacgtgga ctagacatgc agaggcctcg tgggttaatg aaaaattaac tacggggctt
      541 ttgtccttct gccacacgac aggataacaa accaccttca cgtcatgagg cagaaagcct
      601 caagcgccgg gcacatcata gcccatatac ctgcgtactg accacactca ctttccctga
      661 aaataatccg gtcgttcagc cagttcacgg gcaatcagtt catccgcaaa cagtggtgca
      721 tgcctgttga tacgtttctc cagattccgc cgccgtaccg cagccttacg ttcttcgctc
      781 cagcggcgca ccgggcggtc agaaataaaa tccagacaga ccgcataccc tgctccggca
      841 acccagcgtg acataacgga tgccggtgca ggctgccccg cctccaccac ttctgaaacc
      901 agttcatgct cgccaggaca gggataaggc agtttccagc gcagtgagta acgccacttc
      961 acgccgcctc ccggagtttg ttcggcacca tccatcgctc catctcacgg acggcatgac
     1021 cggcctccct tgccgccata cctgcctctg cgatgacctg ccagaacggc atatcctgcc
     1081 actgccagcg ttccattccg gccagtgacg gaaaacgctc gccccactgc cagaacaggg
     1141 tttccaggtc atgctctgcc acccggtcac tccagcgtgc atgccagctt ccgactgttt
     1201 ccggtgaatg gaacgccagt tcgatttctg cctgagcaac cagcgcctgg tacgcacggg
     1261 ttttctgctc tgcctccctc gcctgctggc ggctgtactt ctgcatgacc agcgcagatt
     1321 tatggcgacg gcgttccctg acacggaacc gcaccgacgg gaacaagcgt tctgctgcat
     1381 cgccgggaag ggggaggagg cagacctcgc cctgtgtttc aatcagcaca tcgatggcat
     1441 acagccactg aagacggttg ccgtgcagtt cttctgcggt cagggacggc atgaaaaatc
     1501 gcaggtcaga cagcgaaatc cagcggcttc cgttcagaca gcggaaaaac gcccccgcat
     1561 acggatattc cgccagcgtg tttccctgat ggcggcgttt ttccgtctcc gccaccgccc
     1621 gccatgcggc ctgttgctct gcgctgaggg gaaccagtgc ctgtgaacgt acgctcatgc
     1681 ctgacctcct tccgccatca ggatatggat gatgtcactg acgctgtacc cctgcgcatc
     1741 cagtgacgcc gccagtgcca gtgaatggct gacggcttcc ggtacaaacc gccgctgcac
     1801 ctgaaccacc gcaaacggct caccgccggc attcaccaga accagcaccc agacctgaga
     1861 gacgtcaccc ggtgaacata acgccaggtg aaaacgttcg tgggcaggcg tgaaacggac
     1921 ctggacgggg aaaaagccgc caaactcgct gccgtactcg ccgttcatcg tccagttatc
     1981 cggcgcatcc agctcccgca tcacggcgtg tgtcagctca cgacgttcac cacttccggc
     2041 agcccggata tcttcatatt cctgtgcgct catggtctgt aatacatcca gggtcagttc
     2101 agttttcatc acggtatctc ccttgctgcc gccccgtctc cggcggggcg gtgtggttgt
     2161 tcatcaggca gcatcagcca ggttagtggt gtgttcagtg gcatccatct gtggacgtgg
     2221 tgtacacatc cagccaggca cccagcggtt gtctttcata tggaactctg catgttcagc
     2281 cgcatcgcct ttcttcat
//
LOCUS       DAZGXZ010000294         2261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000294 DAZGXZ010000000
VERSION     DAZGXZ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.296"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..690
                     /locus_tag="ACHKJF_005430"
     CDS             <1..690
                     /locus_tag="ACHKJF_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952593.1"
                     /translation="NGDDIPDKVKFLNTVGAARVYGRDIHTETGEWTTSEFVAWLKEK
                     GAFDQPYWMMKASLLAEFNKVITDVGPGKLNLGGCAIEVMGKYEAAIVRVTIGEYGAT
                     GFINGTVCTCTVYGDTQYFHWRVDYSTKNKPTTASLTVNGWERDEVTGRLRQWGSIEV
                     SEDDGKLMTQHSIRFPVAFPVAALNAQVSAVGDPNVIKMYTLSNPSLVSVTLITVRGC
                     YGKFSWEAIGY"
     gene            700..924
                     /locus_tag="ACHKJF_005431"
     CDS             700..924
                     /locus_tag="ACHKJF_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952594.1"
                     /translation="MEQYVFSPSENMFYPLSLRPVYEAAGRWPEDGIVVDYVVYKVFA
                     ADAAPAGMKRGVGAEKMPVWVPVSEEGTET"
     gene            924..1589
                     /locus_tag="ACHKJF_005432"
     CDS             924..1589
                     /locus_tag="ACHKJF_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0952595.1"
                     /translation="MHIRNFSYYTPVEPDVAGAMYLKSEDGQDWYECQALFSPETLKV
                     VYDSRGVITGYGKDTALLWPVNQSVAEVPDTPENRKIDLSGRWGFDGEKITDLLTAEK
                     ARGMKGDEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEAFFW
                     TDAENNDVPVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLTSADEILAYRV
                     DWK"
     gene            2081..>2261
                     /locus_tag="ACHKJF_005433"
     CDS             2081..>2261
                     /locus_tag="ACHKJF_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952596.1"
                     /translation="MNKKFSKTILSSVVAGLLLVSSGGVAKEIDIGGKYKVILTEETG
                     SARIVDINTKKDLFDG"
BASE COUNT          624 a          422 c          688 g          527 t
ORIGIN      
        1 aacggtgacg atatcccgga taaggtgaag tttctgaaca ccgtgggagc agccagagta
       61 tacgggcggg acattcatac ggagaccggt gaatggacca cgagtgagtt tgtggcctgg
      121 ctgaaagaaa agggggcatt tgaccagcct tactggatga tgaaggcatc actgcttgcg
      181 gaatttaata aggtcatcac ggatgtcgga ccgggaaaac tcaatctggg aggctgcgcc
      241 attgaggtga tggggaagta tgaggcagcc atagtccggg tcaccattgg cgaatacggt
      301 gcaacggggt ttattaatgg cacggtctgt acctgtacgg tttacggcga cacacagtat
      361 ttccactggc gggtggatta cagcacaaaa aacaagccca caacagcaag cctgacagta
      421 aatggctggg agcgtgatga agtaaccggt cgtttgcggc aatggggcag tattgaggta
      481 agtgaggatg atggtaagct tatgacacaa cactctatcc gttttcctgt cgcttttccg
      541 gttgctgctc tgaatgctca ggtgtctgca gtgggagacc ccaacgtcat taagatgtat
      601 accctgtcga atccgtcatt agtgtctgtg actctgataa cagtcagggg atgctacggc
      661 aagttttcat gggaagcgat cggatactga gggcattgta tggaacagta tgttttttca
      721 ccgtcagaga atatgttcta tcccctgtcc ctgcggccgg tttatgaggc agcagggcgc
      781 tggccggaag acggtattgt ggtggattat gtggtgtata aagtctttgc agcggatgct
      841 gcaccggcag gtatgaagcg gggcgtcggt gcggaaaaaa tgccggtctg ggtgccggta
      901 tcggaggagg gcacggaaac atgatgcata tcagaaattt ttcgtattac accccggtgg
      961 agccggatgt tgcaggcgcg atgtacctga agtctgagga tggtcaggac tggtatgagt
     1021 gtcaggcgtt gttttcgccg gagacgctga aggtggtgta tgacagccgg ggtgtgatta
     1081 cgggatacgg taaggacact gcgttactgt ggccggtgaa ccagagtgtg gcagaagtgc
     1141 cggatacccc ggagaaccgg aaaattgacc tgagcgggcg ctggggtttt gacggggaga
     1201 aaatcacgga cctgctgacc gcggagaaag cgcgagggat gaagggcgat gaaataaacg
     1261 cgtggcgtaa tgcgatggaa gcggcgaact acacgtttga gcacaatggg cgtaaatggg
     1321 actacgggaa gtcaacgcag acgcgtcttg agccgtcggt ggcggcagcg aaagcgggga
     1381 aacttccgga ggcgtttttc tggacggatg cggaaaacaa tgatgtgccg gtgacagcag
     1441 aagagcttat tgcgctgagt gaagcggcag agcaggcgat gttcaccaaa gggatggaaa
     1501 tccacgttcg ccagcgcacc atgaagaagg agctggaaaa gctgaccagt gcggatgaga
     1561 tactggcata cagggtggac tggaaataaa aagaagggga gcggatgctc ccctgatatc
     1621 tgagtagtat gctgtttatc gttatcattc atcaaaagaa tattaccgta tggcacggca
     1681 gcagaacatg aaatttgtct cagaactgca aatcagcatt acgggtagtc aggcagataa
     1741 cacaacggga taaaaaaaca gcaaaatgca ttgataaaaa atttttacag gtaggttacc
     1801 gggcgttatg tgacgcatat caaaaacaga actgtaacgg gagataacag aatgatggat
     1861 tctggcgtgg agtaacggcg caatgcgttc cgggatggtg tttctgagaa tgtggctgaa
     1921 aacgcccaca atacagtaag aaaaatccta atcgattata ttaattttta cggaactatt
     1981 cggaataccc ttatgaaagg caacgaagtg tcagtatttt taatgtgttc tgacacgcgt
     2041 tgctctgttt gtatacaaat catttcatag ggtataccag atgaacaaaa aattctctaa
     2101 gactattctt tcttctgttg ttgccggact gttgctggta agtagtgggg gtgttgcaaa
     2161 agagattgat attggtggta aatataaagt tattctgaca gaggaaacag gttcggcaag
     2221 gattgtggat ataaatacta aaaaagattt atttgatgga a
//
LOCUS       DAZGXZ010000295         2238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000295 DAZGXZ010000000
VERSION     DAZGXZ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.297"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1149
                     /gene="traB"
                     /locus_tag="ACHKJF_005434"
     CDS             <1..1149
                     /gene="traB"
                     /locus_tag="ACHKJF_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFP0952597.1"
                     /translation="QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQTPASPPGPE
                     GEPQPGNTPVSFPPQGTGAVPPPTAFYPGNGTTPPPQVTYQSVPVPNRIQRKTFSYDA
                     DRKKGPSLPYIPSGSFAKSVLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDA
                     TGCFVGLEAWGDVSSERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRN
                     GKILGWAWGAGFVDGIGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLS
                     DYYIKRAEQYHPVIPIGAGNEVTVVFQDGFQLKTVEEMALEQSQSQAEEGNAESPVPN
                     PPTAESHLNGFNTDQMLKQLGNLNPQQFMSGDRNQQGGDAGKEGGNGG"
     gene            1139..1705
                     /gene="traP"
                     /locus_tag="ACHKJF_005435"
     CDS             1139..1705
                     /gene="traP"
                     /locus_tag="ACHKJF_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFP0952598.1"
                     /translation="MVGDALIFRVAFVVRWLLWVVRFVVIWPLATMALMALFVLWKDN
                     TTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAADAADYVRE
                     TDRSLMQLVTAFWATLALMYMSFAAGTGKYPMWPGKMKCVRVAAADERLKGVYTEDAS
                     LPEKIRKCPVYFPDDRAIRNNGDKNEHA"
     gene            1644..1916
                     /locus_tag="ACHKJF_005436"
     CDS             1644..1916
                     /locus_tag="ACHKJF_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFP0952599.1"
                     /translation="MFISLMTEQSEITEIKMNMRNLNIIKSSPQSLPHNFNIAAVGLM
                     KKGMSMSLFSSPGHIMVVGQTCAGKATLTAELISAVVGNNKKGEGQ"
     gene            1913..>2238
                     /gene="traV"
                     /locus_tag="ACHKJF_005437"
     CDS             1913..>2238
                     /gene="traV"
                     /locus_tag="ACHKJF_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFP0952600.1"
                     /translation="MKKISLFVPLLGTLLLSGCAGTNSDFECDATTSDTCMTMEQANE
                     KAKKMEQPADAKPVAASLPRLADGNFLTTSVQAVAATTPSRSRPAVTALPEQKLLAPR
                     PLFTAAR"
BASE COUNT          544 a          557 c          668 g          469 t
ORIGIN      
        1 cagcgtcgta ttgaaaagct gggacaggac aatgccgccc tggcagagca ggtaaaagcc
       61 ctgggtgcaa atcccgtcac cgcgaccggt gagcctgtac cgcagacgcc agcctcaccg
      121 cccgggccgg aaggcgaacc acagccagga aacacacccg tatctttccc tccgcagggc
      181 accggtgctg ttccaccgcc gacggcgttt tatccgggga acggcacgac accgcctccg
      241 caggtgacat accagtctgt gccggtaccg aaccgtattc agcgtaaaac gttcagctat
      301 gacgctgaca gaaagaaagg cccctccctg ccgtatatcc cgtccgggag ctttgcaaaa
      361 tccgtcctga ttgagggggc tgatgccaat gcgtctgtca ccggtaatga atccactgtg
      421 ccgatgcagc tgcgtatcac cggcctggtg gaaatgccga acagcaaaac gtatgacgcc
      481 acgggatgtt ttgtgggtct ggaagcctgg ggggatgtgt ccagtgagcg tgccattgta
      541 cgcacccgca atatcagctg cctgaaggac ggcaaaacta ttgatatgcc gattaagggg
      601 catgtcagct tccggggtaa aaacggtatc aagggtgaag tggtgatgcg taacggcaaa
      661 atcctcggct gggcatgggg cgcgggattt gttgacggta tcggtcaggg aatggagcga
      721 gcttcacagc ctgctgtcgg gctgggagcc actgccgctt acggagccgg cgatgtcctg
      781 aaaatgggta tcggcggcgg tgcatcgaaa gcggcacaga cgctcagtga ctactacatc
      841 aaacgtgctg agcagtatca cccggtgata ccgattggtg cgggcaatga agtgacggtg
      901 gtgttccagg atggcttcca gctgaaaacc gtggaagaga tggcgctgga gcagtcacag
      961 agccaggcgg aggaggggaa cgctgaaagc ccggtgccga atccgcccac ggcagaaagc
     1021 cacctgaacg gttttaacac cgaccagatg ctgaaacagc tgggcaacct gaatccgcag
     1081 cagtttatgt ccggagacag gaatcagcag ggcggggatg ccggtaagga gggcggaaat
     1141 ggtgggtgat gcactgatat tccgtgtggc gtttgtcgtt cgctggctgc tgtgggtcgt
     1201 caggtttgtg gttatctggc cgctggcgac aatggccctg atggctctgt ttgttttatg
     1261 gaaggacaac accacgccgg ggaaattact ggttaaggag attaacttcg taagacaaac
     1321 agcacctgcc ggacagttcc cggtcagtga atgctggttt tcttcctctg attcttcagg
     1381 cagatcagaa atacaggata tctgccatta ccgcgcggct gatgctgcag attatgtcag
     1441 ggagactgac cggtcactga tgcaactggt caccgcattc tgggcaacgc tggcactgat
     1501 gtatatgtcg tttgcggcag gcaccgggaa atatccgatg tggcccggaa aaatgaaatg
     1561 tgtccgggtg gcagccgcag atgaacgtct gaagggagtt tatacggaag acgcttccct
     1621 gccggaaaaa ataaggaaat gccctgttta tttccctgat gacagagcaa tcagaaataa
     1681 cggagataaa aatgaacatg cgtaatctta atattattaa aagcagtcct caatcactgc
     1741 cgcacaattt taatatagcc gctgtcggtt tgatgaagaa ggggatgtcg atgagtttat
     1801 tttcttctcc ggggcatatt atggttgtcg gacaaacctg tgcggggaaa gcaactctga
     1861 ctgcagagtt aatttctgct gttgtgggga ataataaaaa aggagaaggg caatgaaaaa
     1921 gatttcttta tttgttcctc tgctgggaac cttattactt tccggctgcg ccggaacgaa
     1981 ctctgatttt gagtgtgatg ccaccacgtc agatacctgc atgacaatgg aacaggccaa
     2041 cgaaaaagcc aaaaagatgg aacagcctgc agatgcaaag ccggttgcgg catcactgcc
     2101 gcgcctggct gacgggaact tcctgaccac gtcggtgcag gccgttgcgg cgacaacgcc
     2161 gtcccgcagc aggcctgctg tgacagcact tccggagcag aaactgctgg caccacgccc
     2221 gctgtttacg gctgcccg
//
LOCUS       DAZGXZ010000296         2212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000296 DAZGXZ010000000
VERSION     DAZGXZ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.298"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..915
                     /locus_tag="ACHKJF_005438"
     CDS             <1..915
                     /locus_tag="ACHKJF_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP0952601.1"
                     /translation="PADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQ
                     PIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKN
                     MNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQV
                     ETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFT
                     VGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSL
                     LINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            982..2212
                     /gene="rhsD"
                     /locus_tag="ACHKJF_005439"
                     /pseudo
     CDS             982..2212
                     /gene="rhsD"
                     /locus_tag="ACHKJF_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
BASE COUNT          485 a          601 c          724 g          402 t
ORIGIN      
        1 ccggcagacc aagacagttc gtgctggatc cgtgtggcac aggcgtgggc aggcaccggt
       61 tttggccacc tggcgatacc gcgtgtgggt caggaggtga ttgtggactt cctcaacggc
      121 gatccggacc agccgattat tatggggcgt acctaccacc aggaaaaccg cacacccggc
      181 agcctgccgg ggacgaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
      241 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcag
      301 aagaacatga acaccgaggt gctgaataac cgcaccacgg atgtgataaa caaccatgct
      361 gagaccattg gcaacaatca gatgattgcg gttaccaaca atcagataca gacggtgggc
      421 gttaaccaga tagagacggt gggcagtaac cagatcatta acgtgggttc tgttcaggtg
      481 gaaacgattg gacttgttcg tgcgctgacc gtgggcgtgg cgtaccagac gacggtaggt
      541 ggcattatga acacttcggt ggctctgatg cagtcctcgc agatcggttt gcataaatcg
      601 ttgagggtcg ggctgggtta tgacgtcaaa gtcggaaata acgttacctt caccgttggt
      661 aaaacgaaaa aggatgatac cgggcagacc gcgatttact ccgccggtga gcatctggag
      721 ctctgctgtg gtaaggcaag gctggtgctg acgaaggacg gacaaatttt tctcaacggc
      781 acaaaaattc atttgcaggg taaggagcag gttaatggtg actcactgtt gattaactgg
      841 aactgtgcag cctcgaaatc cccaccgaag acccctgatg aaaagcagga tacgccggat
      901 atgagagagt actgatcccg tacctgttcg gtattcagta acaaacaagc attaaaaata
      961 aacagaagaa ggaattgaat catgagcgga aaaccagcgg cgcgtcaggg agatatgact
     1021 cagtatggcg gtcctattgt ccagggttcg gcaggtgtaa gaattggcgc gcccaccggc
     1081 gtggcgtgct cggtgtgtcc gggcgggatg acttcgggca acccggtaaa tccgctgctg
     1141 ggggcgaagg tgctgcccgg cgagacggac cttgcgctgc ccggcccgct gccgttcatt
     1201 ctctcccgca cctacagcag ctaccggacc aggacacccg cgccggtggg agtgtttggc
     1261 cccggctgga aagcgccttc tgatatccgc ttacagctac gcgatgatgc actggtactc
     1321 aacgacaacg gcgggcggag cattcacttt gagccgctgc tgccggggga ggcggtgtac
     1381 agccgcagcg agtcaatgtg gctggtgcgc ggtggtaagg cagcgcagcc ggacggccac
     1441 acgctggcgc ggctgtgggg ggcgctgccg ccggatatcc ggttaagccc gcatctttac
     1501 ctggcgacca acagcgcaca ggggccgtgg tggatactgg ggtggtcaga gcgggtgccg
     1561 ggtgctgagg acgtactgcc agcgccgctg ccgccgtacc gggtgcttac cgggctggca
     1621 gaccgtttcg ggcggacgct gacgtaccgg cgtgaggccg caggcgacct ggccggggaa
     1681 atcaccggcg tgacggacgg tgccgggcgt gagttccgtc tggtactgac cacacaggca
     1741 cagcgggcgg aagaggcccg cacctcttcg ctatcttctt ctgacagttc ccgccctctc
     1801 tcagcctcac cgttccccga cacactgccc ggtaccgaat acggccccga caggggcatc
     1861 cgtctttcgg cggtgtggct gatgcacgac ccggcatacc cggaaagcct gcccggtgcg
     1921 ccactggcgc ggtacacgta tacggaagcc ggtgaactgc tggcggtata tgaccgcagc
     1981 aatacgcagg tgcgcgcttt cacgtatgac gcgcagcacc cgggccggat ggtggcgcac
     2041 cgttacgcgg gaaggccgga gatgcgctac cgctacgacg ataccgggcg ggtggtggag
     2101 cagctgaacc cggcaggcct gagttaccgc taccagtatg agcaggatcg catcggacag
     2161 cctgaaccgg cgtgaggtgc tgcatacaga aggcggggcc gggctgaagc gg
//
LOCUS       DAZGXZ010000297         2160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000297 DAZGXZ010000000
VERSION     DAZGXZ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.299"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1230
                     /locus_tag="ACHKJF_005440"
     CDS             <1..1230
                     /locus_tag="ACHKJF_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat domain-containing protein"
                     /protein_id="HFP0952602.1"
                     /translation="RYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYGWDGDRL
                     TTIQNDRSRIQTIYQPGSFTPLIRVETATGELARTQRRSLADALQQSGGEDGGSVVFP
                     PVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIHLYHCDH
                     RGLPLALVSTEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHR
                     YYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKP
                     PTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCT
                     PQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDL
                     GEILAKMKQRTSRGIRK"
     gene            1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHKJF_005441"
     CDS             1227..1796
                     /gene="ybfC"
                     /locus_tag="ACHKJF_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP0952603.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIKIQHELIRSEEK"
     gene            2022..>2160
                     /locus_tag="ACHKJF_005442"
     CDS             2022..>2160
                     /locus_tag="ACHKJF_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HFP0952604.1"
                     /translation="MELKKLMEHISITPDYRQAWKVVHKLSDILLLTICAVISGAEGW
                     ED"
BASE COUNT          653 a          452 c          533 g          522 t
ORIGIN      
        1 cgctatcttt acgacccgct gggccgcagg gtggcaaaac gggtgtggcg acgtgaacgg
       61 gacctgacgg gctggatgtc gctgtcacgg aaaccgcaag tgacctggta cggctgggac
      121 ggcgaccggc tgaccacgat acagaacgac aggagccgca tccagacgat ttatcagccg
      181 gggagcttca caccactcat cagagttgaa actgccaccg gtgaactggc cagaacgcag
      241 cgccgcagcc tggcggatgc gcttcagcag tccggcggcg aagacggtgg cagtgtggtg
      301 ttcccgccgg tgctggtgca gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg
      361 gtgagtgagg aaagccgccg ctggctggca tcgtgcggcc tgacggtgga gcagatgcaa
      421 aaccagatgg acccggtgta cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat
      481 cgcggcctgc cgctggcgct tgtcagcacg gaaggggcaa cagaatggtg cgcagaatac
      541 gatgaatggg gcaacctgct gaatgaagag aacccgcatc agctgcagca gcttatccgc
      601 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
      661 gacccgctgc aggggaggta tatcactcag gatccgattg ggctgaaggg gggatggaat
      721 ttttatcagt atccgctgaa tccggttcag tatatagatt caatgggact ggcatcaaaa
      781 tatggacact taaataatgg cggatatgga gcgagaccca acaaaccgcc tacgcccgat
      841 ccaagtaaat tgccggacat agcgaaacaa ttaagactgc catatcctat tgaccaggcc
      901 agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta cgactacaca
      961 ctgtattgca ggaagtgggt aaaaccaaat ctgacttgta cgccacagga tgattcccag
     1021 tatccaggga tggatacaaa gacagcaagt gattacctgc cacagacaaa ttggccaaca
     1081 actcaattac caccaggata tacttgtgca gaaccctatt tattcccaga cattaataaa
     1141 cccgatgggc cagcaacagc agggatagat gatttgggtg aaattttagc taagatgaaa
     1201 cagagaacat cgagaggaat aagaaaatga aaagagtttt gttctttttg ctgatgatat
     1261 ttgttagttt tggtgttata gctgattgcg aaatacaagc taaagatcat gattgtttta
     1321 ctattttcgc taagggtact attttttccg cgtttcctgt cttaaataat aaagccatgt
     1381 ggagatggta tcagaatgag gacatcggtg agtattattg gcaaacagaa ctgggtacat
     1441 gtaaaaataa taaattcaca ccaagcgggg cgagactctt aattcgcgtc gggtcactac
     1501 gccttaatga aaatcatgcc atcaaaggaa cgttgcagga gttgataaat acagcagaaa
     1561 aaacggcgtt tctcggcgat cggtttagaa gttatataag agcgggtatc tatcaaaaaa
     1621 agagcagtga tccagttcag ttgttggcag tccttgataa ttcaatcatg gtaaaatatt
     1681 ttaaagatga gaaaccaact tatgctcgaa tgacagcaca tctcccgaac aaaaatgagt
     1741 cttacgaatg tttaataaag atacagcatg aactaattcg tagtgaagag aaataaaatc
     1801 tcttcctgag aaaacaaatt aatcattgtg aaaaattata taactcatgg aataggtgtt
     1861 tattttgcgt tccatttgca gggaaagatc acgtaacgct acttttttgt actaaataat
     1921 tcgaatttta agttcaacaa ttgagatact acttattgtc taaagctgtt tttcatagct
     1981 tatacatgat caaatactcc ttacataaat aaggtgaaca aatggaactt aaaaaattga
     2041 tggaacatat ttctattaca cccgattaca gacaagcctg gaaagtggtg cataaattgt
     2101 cagatattct actgttgact atttgtgccg ttatttctgg tgcagaaggt tgggaagata
//
LOCUS       DAZGXZ010000298         2097 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000298 DAZGXZ010000000
VERSION     DAZGXZ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.300"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..95)
                     /locus_tag="ACHKJF_005443"
     CDS             complement(<1..95)
                     /locus_tag="ACHKJF_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062863141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase cytotoxin subunit B"
                     /protein_id="HFP0952605.1"
                     /translation="MTIKRFFVCAGVMGCLSLNPAMAEWTGDARDG"
     gene            complement(112..1155)
                     /gene="subA"
                     /locus_tag="ACHKJF_005444"
     CDS             complement(112..1155)
                     /gene="subA"
                     /locus_tag="ACHKJF_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP0952606.1"
                     /translation="MLKILWPHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKESEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWIRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWTELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSMNKKRDPVIRV
                     AALAQYRKGETPVLHGGGITGSRFGNGWVDIAAPGQNITFLRPDGKTGTGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEGRVLNAEKAISMFCKKN
                     YIPVRQGRMSEEL"
     gene            complement(1861..>2097)
                     /locus_tag="ACHKJF_005445"
     CDS             complement(1861..>2097)
                     /locus_tag="ACHKJF_005445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952607.1"
                     /translation="ISGLFYISPAKVIRHAGYIIRESGSERRYLLPQSYLINPYQQGN
                     SMAKSSNYAPVKVIIYVDIGKDPCHTVIINRSDN"
BASE COUNT          588 a          509 c          436 g          564 t
ORIGIN      
        1 ccatcccggg catcgccagt ccactcagcc atggcaggat taagggataa acatcccata
       61 acacctgcac atacaaaaaa cctcttaatc gtcatattca cctccggaat tttacagttc
      121 ttcactcatc cttccctgac ggacaggaat ataatttttc ttgcagaaca tgctaattgc
      181 tttttctgca ttaagaaccc tcccttccgt caccttatct gcaagtgagg ggtatttgtc
      241 tgcagactcc agcagtgtac gcttcagttc tgtcgctgtt gcacggggat tacatgagac
      301 catcgctgcc agaacgccgg aaactatagc tgttgcttcc gaggttccac tacctgtccc
      361 cgtttttcca tctggtctga ggaatgtaat attctgtccc ggtgcagcaa tatcaaccca
      421 gccgtttccg aaccgacttc cggtgatccc tccaccatgc aatacaggtg tttctccttt
      481 ccggtactgt gccagagctg ccactcttat cacaggatcc cgctttttat tcatactgct
      541 gaccggatgg taagccgcag gccatattct ctgctgagca ctcagttttc gtatatctgc
      601 gccatcatta cccactgccg caacaattaa tcgctcatta ttccggccca tacgggaaag
      661 cagttcggtc cagaccgatg cggaagatat ccccttctgg cctcccgata tattaatgat
      721 tttctcttct cctggtgcaa gaaaaacatt tgacataatg ctttcagttg ctctaatcca
      781 tgagtcctgt acaccgtcag gaataaccct tctgctggat atcagtgcat gaggataaac
      841 accataaacc ccatgacgtg aggcaatgag tgaagccatg gcagtaccat gaatatataa
      901 agcttcagac tcttttaccg ggaatggcga accatcctga gtaaaactga atttggcaaa
      961 ttcgctgtcg ctgaggcctc ctacaaatgc tactccggaa tcaacaacag atacgacaac
     1021 aggggtattc ttgtctgtga gcgacatggt tgtttctgtc aggccaattg catcaaaata
     1081 ccagggtttt tctgccctga cagacacaga tataaaaagc aggaacagaa tatgcggcca
     1141 taaaatctta agcacaatat cctccagtca gtacaaataa acagtattgc aggtcaatat
     1201 cttacaggaa catcacatct gaaatatcct gttttcgaca aaagataaac aatcacaacc
     1261 aataaagcga gtgaagcccg tctcaaaaac attaattttt atatttatgt atccaatgaa
     1321 ttaacgctca ccttcacggt aatatgcatc tggatttctg caacaggtca gcgataagat
     1381 gctctgcctc ctccttcaga gcagaagtaa atacagccat gatggataaa acttaaccag
     1441 aaaaaataag aggaggcaag taagttgcct ggcatgttta tgacaataaa aagttccgtc
     1501 aggcatcatg gcgaacagga tatcacagcg ccgttttacc ggggcaatca gtgaatggtg
     1561 gtgtggttca ttggtaagcc ccggatatca ggtcactgag tatattcatg aatgaccgtt
     1621 aaaccgggga tgcgagctgc tccccgggaa agtcgaactg actggtcaat agatatcaat
     1681 acggggcagt cagtccactt acaggtatct gacaaggaca ccttctgagc gtgcaatgtg
     1741 gacgggtaat acaacgatgg tagcaggctg gtcagcaatc agcagtatat gcccatattt
     1801 cctgaggccg gatatctgtt tcctgagctt gttttcgacg tttgctaata ttttgtcgaa
     1861 ctaattatca gaacgattaa tgataacagt gtggcaggga tctttaccga tatcaacgta
     1921 aataatgacc ttaacaggtg cgtaattgct ggattttgcc atgctgttac cctgttgata
     1981 cgggttaatt aaataacttt ggggaagcag gtaccggcgt tcactaccgc tctccctgat
     2041 aatgtatcct gcatgcctga taacttttgc cgggctaatg taaaataacc ctgaaat
//
LOCUS       DAZGXZ010000299         2094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000299 DAZGXZ010000000
VERSION     DAZGXZ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.301"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            73..266
                     /locus_tag="ACHKJF_005446"
                     /pseudo
     CDS             73..266
                     /locus_tag="ACHKJF_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335629.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            308..637
                     /locus_tag="ACHKJF_005447"
     CDS             308..637
                     /locus_tag="ACHKJF_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0952608.1"
                     /translation="MFSVVYHPEAREEATALPIKIRVKFDRLISKLEYDARLLREPDT
                     KPLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEV
                     LNETGKP"
     gene            618..899
                     /locus_tag="ACHKJF_005448"
     CDS             618..899
                     /locus_tag="ACHKJF_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952609.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDN
                     LTKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVITLY"
     gene            1082..1366
                     /locus_tag="ACHKJF_005449"
     CDS             1082..1366
                     /locus_tag="ACHKJF_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFP0952610.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVISGQIIDHQAVQAWADSLSTDNPLPVPR"
     gene            1366..1641
                     /locus_tag="ACHKJF_005450"
     CDS             1366..1641
                     /locus_tag="ACHKJF_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0952611.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARMVQSLIQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGLRLGNVQYCTVALLR"
BASE COUNT          573 a          502 c          534 g          485 t
ORIGIN      
        1 aacgcggaga tacgccccga cttcgggtaa accctcgtcg ggaccactcc gaccgcgcac
       61 agaagctttg tcatggctga aagcaggtat ggcttagcag gatggggatg ggtaaggtga
      121 aatctgtcaa tcagtaccgg cttacgccgg gcttcggcgg ttttacacct gtgctacatg
      181 taaccactgt gtgccgcctt acatgcgctg gcgcgcataa acaacaagtt gctacattag
      241 cttacaggtt aattcacctt gcataagatt agcatatagg ttaatgttta tcgaaatggg
      301 aggtcatatg tttagcgttg tttatcaccc ggaagccagg gaagaagcca cagcattacc
      361 cataaaaatc agggtgaagt ttgaccgtct catcagcaaa ctggaatatg acgcccgatt
      421 actgcgggaa ccggatacaa agcccctggg ggatggactt tttgagatcc gcacgatggg
      481 gacggacata gccagaggca tctgggtata ccacaaaggc aataccatca tcatgctccg
      541 ggttttcatc aagaagtctc agaaaacgcc agcaaaagaa atcgatctcg ccaaaaagcg
      601 gttagcggag gtacttaatg aaactggtaa gccataaaga actgcataac gagtggatgc
      661 aggacgacga atatcgcgct gcatggcagg aagaagaacg taaggagaaa ctcagggagt
      721 tgcttgcaga atggcgtaag catgacaatt tgacaaaagc gcaggttgct gaacgcatgg
      781 gggttactcc tcctgttatt tcccgcctgg aaaacaacat cactaaagcc agcatcgaca
      841 ccctgactcg ctatgcccat gcgtgcggta tcaaaaaacc tgttattacg ctgtactgaa
      901 ctctgctcta tggctgccag ttctggagaa atcagtaact aaattttaat tagctacgtt
      961 atggtaaatc tgcctgatgt gtcgatttct ctgaaagtaa ttcggcttct gtgggctgcg
     1021 tttgccttgt gccacctgaa tgtaatcact atgattaaat atgattaaca actaatcgga
     1081 tatacaaatg aaaaacaata ccgcacaggc aacaaaagta attaccgcgc atgtgccatt
     1141 acctatggct gataaagtcg accagatggc cgccagactg gaacgctccc ggggctgggt
     1201 tatcaaacag gcactttctg catggcttgc ccaggaggag gagcgtaatc gcctgacgct
     1261 ggaagccttg gacgatgtga tatccggaca gattatcgac catcaggctg tacaggcctg
     1321 ggcagacagc ctcagtactg acaatccgtt accggttcca cgctgatgga actgaagtgg
     1381 accagtaagg cgctttctga tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa
     1441 cctgccgccg ccagaatggt acagtccctg atacaggcac cggtcattct gttaactcat
     1501 ccacgtatgg gagaacagtt gtttcagttt gaacccaggg aggtcagacg gatttttgct
     1561 ggcgagtacg aaatccgtta cgaacttaat gggctgcgtt tgggaaacgt acaatattgt
     1621 acggttgcgc tattgcggta gggtgtactc caacgctata cggtatttgg cagaccttta
     1681 gggacatgag ataacctaaa tgcaataaga gaaaagcaat aacttaaagg cgatagctga
     1741 tcacgatgaa tcaatcagtt acttatccgc atcgattaaa aaatgctcct aacgtacaat
     1801 aattccgttt cccaaaccga cccctcagtg ttctggctgg aagtcctcag tacacgctcg
     1861 taagcggccc tgacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaccctcgt
     1921 cgggaccact ccgaccgcgc acagaagctc tctcatggct gaaagcgggt atggcttaac
     1981 agggtgggaa tgggataggc gaaatctatc aatcagtacc ggcttacgcc gggcttcggc
     2041 ggtatcacat gtaaccacag cgtaccgcct tccatgccgc tggcgcggca tatc
//
LOCUS       DAZGXZ010000300         2093 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000300 DAZGXZ010000000
VERSION     DAZGXZ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.302"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1940)
                     /gene="espI"
                     /locus_tag="ACHKJF_005451"
     CDS             complement(<1..1940)
                     /gene="espI"
                     /locus_tag="ACHKJF_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFP0952612.1"
                     /translation="MNKIYALKYSSLTGGLIAVSELSKKVTGKTGRRLMTVSLVLSVT
                     LSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIEINDNNGNKVGVLDVPMLDFS
                     SLSRDGHTTLIHPGYVVSAKHGGLQSVSSATFGYDQIYKIVDNNLAGIDFSAPRLNKL
                     VTEVIPADIQGKDKFNNNRYTAFYRAGVGSQYIRYANGTDKLLQAYTPEKAYLTGGTV
                     GKPYYTHYNGMKMISANPGNTFDKNQGPLASYGQSGDSGSPLYAWDNIDKKWVLAGVT
                     LHNYGVKGARNDWLLIPHDFISQKLQDDLKPIIVASPEENILRWEFDRSRGTGTLSQG
                     EKIFSMTGSVNGNANTGNNLVFSGNEGKIELVSSVEQGAGYLQFDKDYTVLTNNNSTW
                     TGAGIIVGDEANVKWGVNGIAGDNLHKVGSGTLTVNGHGENKGGLKVGDGVVVLEQQP
                     DANQKQQAFSHINIASGRATVKLNGANQVDADNISWGYRGGKLDLNGYDFTFSRLQAA
                     DYGAEISNDNQTDKSIVTLSLSPLKAEEINVVVNNINIMGGTGKPGDLYYTTFDGNYY
                     LLKSNRYGSALFGALNNQSEWQRLGKDKEKAIGLYTQMKMQESAPLSYIYHGKITGNT
                     SVEIPKLAGNDILTLDGSVSISG"
BASE COUNT          585 a          484 c          362 g          662 t
ORIGIN      
        1 cctgatatac tgacagagcc atcaagcgtt aaaatatcat tgcctgccag tttggggatt
       61 tccacactgg tattaccggt tatttttcca tgatatatat atgataaagg agcgctttcc
      121 tgcattttca tctgagtata taacccaatt gctttttctt tatccttacc cagcctttgc
      181 cattcgctct gattattcag cgcgccaaac aaagcgctgc catatcggtt acttttcaac
      241 agataataat ttccgtcaaa ggtcgtataa tacagatcac ctggtttgcc tgtccccccc
      301 attatattta tattattaac aaccacattt atttcttctg ctttcagagg agataacgaa
      361 agtgtgacta tggatttatc tgtctgatta tcgttgctga tttcagcacc ataatctgca
      421 gcctgaagac gggaaaaggt aaaatcatac ccatttaaat ccagtttccc accccgatat
      481 ccccagctga tattatctgc atctacctgg tttgccccgt taagtttaac tgttgcccga
      541 ccactggcta tattgatatg gctaaacgcc tgttgtttct ggtttgcatc tggctgttgc
      601 tcaagaacaa caacgccgtc cccaacttta aggccacctt tattctcacc atgaccatta
      661 acagttaatg tcccggaacc aactttatgc agattatcac cggcaatgcc attaactccc
      721 catttgacat ttgcctcgtc accgacaata attccggcac cagtccatgt actgttatta
      781 tttgtcagta cagtgtagtc tttatcaaac tgaagatatc cggctccctg ctccacactg
      841 gataccagtt cgattttccc ttcattacct gagaacacaa gattgttccc ggtatttgca
      901 tttccgttta cactaccagt catggaaaaa atcttctctc cctgactgag agtacctgta
      961 cctctggaac gatcaaattc ccagcgtaag atattctcct ccggagaagc aacaataatt
     1021 ggtttaaggt catcctgtaa tttttgactg ataaagtcat gaggtatcag gagccagtca
     1081 tttcgtgcac cttttactcc ataattatgc agagtaactc cagctaatac ccattttttg
     1141 tcaatgttat cccaggcata taacggcgaa ccactgtctc cactctgtcc ataactggca
     1201 agaggtccct ggtttttatc aaaggtattt cccgggtttg ctgaaatcat cttcataccg
     1261 ttatagtgag tataataagg tttccctact gttccgccgg tcagataagc cttttctgga
     1321 gtgtaagcct gcagtagttt atctgtgcca tttgcataac ggatatattg agagccaacg
     1381 cccgcacggt aaaaagccgt ataccggtta ttattaaatt tatcctttcc ctgtatatct
     1441 gcgggaatta cttctgtaac aagcttattt aatcgtgggg cagaaaaatc tataccagca
     1501 aggttattat caactatttt atatatctgg tcataaccaa aagttgctga tgaaacactt
     1561 tgtaaaccac catgtttagc cgatacaaca taaccaggat gaatcaatgt ggtatgacca
     1621 tcacgagaaa gactggaaaa atcaagcatg ggaacatcaa gaaccccaac tttatttcca
     1681 ttgttgtcgt ttatttcaat tcctgtcact ccgggggtaa acacaccttt attttcagca
     1741 aagtcacgaa aagtctgata tggtatttct gcgctaaccg ttgatgcttt acccggtaaa
     1801 gcagaaagag tcactgataa taccagggaa accgtcatta atcttctgcc ggtttttcct
     1861 gtgaccttct tacttaattc tgacacagct ataagcccac cagtaaggga gctatattta
     1921 agcgcgtata ttttattcat ataattcctg ctatataatt atatggtatt aaaatgtatt
     1981 gtctgtccgg aaagagtgag tactggatac tcaccctgaa tctattttaa atgacatcag
     2041 gaatacctgt ccatacttaa gatttttcct gtggaaaaca aacgttaata tat
//
LOCUS       DAZGXZ010000301         2068 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000301 DAZGXZ010000000
VERSION     DAZGXZ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.303"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..441
                     /locus_tag="ACHKJF_005452"
     CDS             <1..441
                     /locus_tag="ACHKJF_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0952613.1"
                     /translation="LVTGMAEDLQSLVGATVVRRRVYARFLDAVNFVAGNPEADPEQE
                     LTDRWVVEQLSALTAMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYNGPA
                     VADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            452..1195
                     /locus_tag="ACHKJF_005453"
     CDS             452..1195
                     /locus_tag="ACHKJF_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0952614.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAQMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGQNLYLDNLADN
                     GFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            1192..1731
                     /locus_tag="ACHKJF_005454"
     CDS             1192..1731
                     /locus_tag="ACHKJF_005454"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0952615.1"
                     /translation="MNTAAEAIRALSLQVPGFRQKLYEGWYQLRIAGSDVSEQELHSR
                     VCEPLNDGDVIHLVPRTEGAKNGGVLQVVAGAVLAVVGYVFSWTGIGAVIGNLGVAMM
                     VGGITQMLTPRAKTPSATTTDNGKRNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQ
                     DISTRDEGGDGKVVVIGRG"
BASE COUNT          461 a          487 c          658 g          462 t
ORIGIN      
        1 cttgtcaccg ggatggcgga ggatttgcag agcctggtgg gtgccacggt ggtccgccgc
       61 cgggtgtatg cgcgttttct tgatgcggtg aactttgtgg cggggaatcc ggaggcagac
      121 ccggagcagg agctgacgga ccggtgggtg gtggagcagt tatcagcgct gacggccatg
      181 acggcctcgt ttgtgctggc gacaccgacg gagacggacg gtgcgctgtt tcccggtcgc
      241 atcatgctgg cgaacacctg tatgtgggat taccggggcg atgaatgcgg gtataacggt
      301 ccggcagtgg cggatgagtt cgacaacccc accacggata tccgcaagga cagatgcagt
      361 aaatgcatgc gcgggtgtga gatgcgcggc atggtggcta atttcggcgg tttcctttcc
      421 attaataaac tttcgcagta aatcccgttt tatgacacag actgaatcag cgattctggc
      481 gcatgcccgg cggtgtgcgc cagcggagtc gtgcggcttc gtgataagca ccccggaggg
      541 cgaacggtac cagccctgcg tgaatatctc cgcagagccg gaggcgtatt ttcgtattgc
      601 accggaagac tggctgcagg cacagatgca gggggagatt gtggcgctgg tccacagtca
      661 tcccggtgga ctgccctggc tgagcgaggc ggaccggcgg ctgcagataa agagtgccct
      721 gccctggtgg ctggtctgcc ggggtgaaat tcaccggttc cgctgtgtgc cgcacctgac
      781 cggacggcgc tttgaacacg gtgtgacgga ctgttacacc ctgttccggg atgcatacca
      841 tctggcgggg ataacgctgc cggattttgt gcgtgaggat gactggtggc gcaacggtca
      901 gaacctgtac ctggacaacc tggcggacaa cggcttttac cgggtgtctc cgtcctgtgc
      961 acaggcaggc gatatcctgc tgtgctgctt tggcgcatcg gtgccgaatc atgccgccat
     1021 ttactgtggc aacggtgagc tgcttcacca tatacctgaa caactgagta aacgggagag
     1081 gtattcagag aaatggcaac gacgaacgca ttctgtctgg cgtcaccgcc actggtccgc
     1141 atctgccttc acggggattt acaacgattt ggtcgccgca tcagcctgta tgtgaacacg
     1201 gcagcggagg ccatccgtgc cctgtcgctg caggtgccgg gattcaggca gaaactgtat
     1261 gagggatggt atcagttacg cattgcgggc agtgatgtat ctgaacagga gcttcattcc
     1321 agagtatgtg agccgttgaa tgacggggat gttatccatc tggtgccccg gacggagggg
     1381 gcaaaaaatg gcggtgttct tcaggtggtg gccggtgctg ttctggctgt tgtgggatat
     1441 gtgttcagct ggaccggtat tggtgctgtc atcggtaatc ttggggtggc catgatggtg
     1501 gggggtatca cgcagatgct cacccccaga gcgaaaacac cgtctgccac gacaacagat
     1561 aatggtaaac ggaacacgta tttctcctca ctggacaaca tgattgccca gggtaacccg
     1621 atgccggtgc cttacggtga aatgctggtt ggttcacgac ggatatccca ggacatcagc
     1681 acccgtgatg agggcggaga tgggaaagtg gtggttatcg ggcggggata aaaataaaaa
     1741 aatcccgcag agttagcgga gctgcgggaa gagatacgaa gattaactgt aaggagttat
     1801 tattatgtca cagcaaaaaa aacggtaacg cagagaaatt ataagcgcca ctgggatttt
     1861 gtgaaaatgt gaagaatttc agaaattttc tgttgcggag ctgaactaaa aaacgctccc
     1921 gcaatacacc tggatattaa cgggaggaac gaatagtcac cttaaggagt tgcgattaat
     1981 tgcttttatt gaccgcaggt aatctaccca tccggcaagg tttcctccac gggaatttgc
     2041 cggaaatgtg aagaatttca gaaatttt
//
LOCUS       DAZGXZ010000302         2061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000302 DAZGXZ010000000
VERSION     DAZGXZ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.304"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..994)
                     /locus_tag="ACHKJF_005455"
     CDS             complement(<1..994)
                     /locus_tag="ACHKJF_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952616.1"
                     /translation="MGLFNYKDYHQAQITGLISASYNIAVYANTGKVLGLGSFLVSRL
                     FAKHPDRIDYSTDVLNEGWRDITVEELRLSSEYVDSRGFYRFPSPVTGNPASGFGPQL
                     KVVGLFDAQQNLQKIAISFCGTNDICDIVDYTQLNNGKALSEAENLLQSIRDFAISNG
                     LSGEDVIVTGYSLGGGLTNIMARLSESLADGFYANSDYIAWASPLVFDGDGKVLNIGF
                     ENDPVFRVLGDGAGFWEAVKAMGPGLTTPDREYDSSPDNIVLYTDLYGSFLWNIIPFS
                     VLALPLGSWSAHFAGSKTDAFERILGAQFYDYMSPDSTVIVDEQSSWTSWYSWVH"
     gene            complement(1053..>2061)
                     /locus_tag="ACHKJF_005456"
     CDS             complement(1053..>2061)
                     /locus_tag="ACHKJF_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952617.1"
                     /translation="AFYERALPSNVSGDLYPQPSIFGDKVSSVSKNWSTLLDSNPGSY
                     VTSQRLDSGANQYNYNGHTGSDVISITDSLGGLDRTQASRFPVGLFTGEGNDLIVTGR
                     DYGRNTSAGYTDHSHRTDMGNGDDTLVVGVGNNDVTLYVNEEGQLRATTDSYNGSTSI
                     DYTGLNSSSSGGTISGTDIVMGAGNDTVLALGYEGNSADAIINTNIDLGAGNDFIYAN
                     GEISTNNGTQVNIIGGEGFDTISLDNTTVTSAMFSGFEHVDLHSTSHLILNSDDFKSQ
                     DIEGEILKISGSSGASVDVQNFDWENLSSANDGDVKYFTYQSTDIPGLTLWIQEGIEV
                     K"
BASE COUNT          633 a          490 c          351 g          587 t
ORIGIN      
        1 catgaaccca tgaataccaa gatgtccagc tactttgctc atccacaatg actgtactgt
       61 ccggtgacat gtagtcgtaa aactgagccc ctaaaattct ttcaaaggcg tcagtttttg
      121 aacccgcaaa atgcgcactc cagcttccca gcgggagtgc aagcacagaa aaggggataa
      181 tattccagag gaatgaacca taaaggtctg tgtataaaac aatgttatca ggagaagagt
      241 catattcacg atccggggtt gtcaagcccg gccccattgc tttaactgcc tcccagaacc
      301 cggctccatc tcccagcaca cgaaataccg gatcgttttc aaaaccgata tttaatacct
      361 tcccatcccc gtcaaatacc agcggggaag cccaggctat atagtcagaa ttagcataaa
      421 aaccatcagc cagagactca ctgagtctgg ccataatgtt agtcaaccca ccccccaggc
      481 tatagcctgt gacaataaca tcttcaccac ttaaaccatt actgattgca aagtctctga
      541 tactttgaag caggttttct gcttcagaca gcgcttttcc attattgagc tgtgtataat
      601 caacaatatc gcagatatca tttgttccac agaatgatat tgcaattttc tgtagattct
      661 gttgagcatc aaacaaccca acaactttca actgaggacc aaatcctgac gcagggtttc
      721 ctgtaaccgg actgggaaag cgataaaaac ctcttgaatc gacatattcc gaagataacc
      781 tgagttcttc cactgtaata tcacgccagc cttcattaag aacatcggta gaataatcaa
      841 tcctatctgg gtgctttgca aatagcctgg ataccagaaa acttcctaat ccaagaacct
      901 ttcctgtatt ggcatatact gcaatattat aagacgcaga gatcaggcct gttatttgcg
      961 cttgatgata atctttataa ttaaagagtc ccatgagtat ttccttattt aaagaacagc
     1021 cccatgctgg ggctgtttgt ttagattttc ttttatttaa cctcaatacc ctcctgtatc
     1081 caaagagtta aaccaggaat atcagtggac tgataggtga aatatttaac atcaccatca
     1141 ttggcactac ttaaattctc ccagtcgaag ttttgtacat ccacggatgc acctgaacta
     1201 cctgagattt ttaatatctc cccctctata tcctgagatt taaaatcatc tgaattcaga
     1261 atgagatgag atgtagagtg caaatcaaca tgctcaaaac cagagaacat agcacttgtt
     1321 actgtagtat tgtccaaaga tatcgtgtca aagccctctc cacctataat atttacctga
     1381 gtaccattgt tagttgaaat ctctccatta gcgtaaatga agtcattacc tgcccccaag
     1441 tctatattcg tattaataat agcatccgca ctattacctt cataccctaa agccagaaca
     1501 gtatcatttc ctgcccccat aacaatatca gttcctgata ttgttccacc tgatgatgaa
     1561 gaattgagcc cagtatagtc aatggatgta ctaccattat aactatcagt agttgctctc
     1621 agttgtcctt cttcattcac atatagtgtt acatcgttat ttccgacacc aacaaccaag
     1681 gtatcatctc cattccccat atcagtacga tgagagtgat ccgtgtatcc tgcactggta
     1741 ttacgtccat aatctcttcc tgtaacgatt aaatcattcc cttctcctgt gaataaacca
     1801 accgggaagc gagatgcttg agttctatct aatcctccta aagaatcggt aattgagata
     1861 acatcagaac ctgtatgacc attgtaatta tattgattag ctcctgaatc taatctctgg
     1921 gatgtaacat aacttccagg attactatca agtaaagtag accagttctt agaaacagaa
     1981 gataccttat caccaaatat ggacggttga ggatataagt ctcctgatac attagagggc
     2041 aatgcccgtt cgtaaaatgc c
//
LOCUS       DAZGXZ010000303         2047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000303 DAZGXZ010000000
VERSION     DAZGXZ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.305"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1374
                     /locus_tag="ACHKJF_005457"
     CDS             <1..1374
                     /locus_tag="ACHKJF_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104355618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFP0952618.1"
                     /translation="SGSGNGGGEHGSQTGWGWSKTNNPDVPPYVDDNGQVRITITNGL
                     VKTPVYGVPEAGGNSDVQGGYIPENPNDEVARKWDKNNLPREIDVSIDGFKYRVTLND
                     NGRAIGILRTGVRPYVGSEKAKAGIMEKINHKTPEEIYEALGFNKDESQRQEKAKQQA
                     EDAWDRLPPNVRKFDVDVEQFHYLVVLDDYGNVLSVTRTAVRPYVGSEKAKAGIMDKV
                     DHKTPEEIYEALGFNNEEPQRQNQAKKAAYDVFYSFSMNRDRIQSDVLNKAAELISDM
                     GNKIGDYLGESYKSLAREIADDIKNFQGKTIRSYDDAMESLNKILSNPSFKVNRADRD
                     ALVNAWRSRDAQDIANKLGNISKAFKAADVIMKIEKVKEKSIEGYETGNWGPLMLEVE
                     SWVLSGMAASVAMTLLSAIIGSFTLPALALTAINIAGIMGISYLASYIDASVAEKINN
                     KLVHFTH"
     gene            complement(1397..1930)
                     /gene="cui"
                     /locus_tag="ACHKJF_005458"
     CDS             complement(1397..1930)
                     /gene="cui"
                     /locus_tag="ACHKJF_005458"
                     /inference="COORDINATES: protein motif:HMM:NF015795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein Cui"
                     /protein_id="HFP0952619.1"
                     /translation="MINKHHQTDKGNKIATKMFYFLIISLIPMFSIIIIYIHNPTSNI
                     LNYIALRANDLPAIYSQNTPVFSKTLSLYVKTAPFTAILLFLNTCKNLRLKNDKSPWQ
                     LLKINIFFTFFYAILIYAFLFTDTELTSSAKMLKLMSVNIILLSFFYISLHSIIFIFS
                     YLYFWFCIGSYRSFKEK"
     gene            complement(1982..>2047)
                     /locus_tag="ACHKJF_005459"
     CDS             complement(1982..>2047)
                     /locus_tag="ACHKJF_005459"
                     /inference="COORDINATES: protein motif:HMM:NF037571.4"
                     /note="Members of this family include Cki (colicin K
                     immunity protein) for the pore-forming toxin Cka (colicin
                     K activity protein), as well as immunity proteins from
                     other related colicin systems; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cki family colicin immunity protein"
                     /protein_id="HFP0952620.1"
                     /translation="YLLMAIPVGLPSFIYYSLKRK"
BASE COUNT          676 a          272 c          475 g          624 t
ORIGIN      
        1 tcaggctctg gtaatggagg tggtgaacat ggaagtcaga cggggtgggg atggagtaaa
       61 accaataatc cggatgtgcc gccttatgtt gatgataatg ggcaggtgcg cattaccatt
      121 acaaatggtc tggtaaaaac gcctgtatat ggagttcctg aggctggcgg aaatagtgat
      181 gttcaggggg gctatattcc tgaaaatcct aatgatgaag ttgctcgtaa gtgggataaa
      241 aacaacttac cgcgagagat tgatgtgagt atcgatggtt ttaaatatcg tgtaacgctt
      301 aatgataatg gtagagctat cggtattctt cgaacaggtg tccgtcctta cgtagggagt
      361 gaaaaagcta aggcgggtat tatggaaaaa atcaatcata aaacacctga ggagatatat
      421 gaggcgttag gttttaataa agatgaatct cagcggcagg aaaaagcaaa acaacaggct
      481 gaagatgcgt gggatcgttt gcctcctaat gtgagaaaat ttgatgttga tgttgaacag
      541 ttccactatt tagtggtgct tgatgattat ggtaatgttt tatctgtaac tagaacagct
      601 gttcgtcctt atgtagggag tgaaaaagct aaggctggta ttatggataa agtcgaccat
      661 aagaccccgg aagagatata cgaggcatta ggatttaata atgaagagcc tcaacgacag
      721 aatcaagcta aaaaagctgc ttatgatgta ttttattctt tcagtatgaa ccgtgaccgg
      781 attcagagtg atgtgctaaa taaagcagct gaacttatat ctgatatggg taacaagatc
      841 ggtgattatc ttggtgaatc ttataagtcg ttagcaagag aaattgctga tgatatcaaa
      901 aatttccagg ggaaaaccat ccgtagctac gatgatgcaa tggagtcatt aaataaaatt
      961 ttgtctaatc ctagttttaa agttaacaga gcagaccgtg atgcactggt gaacgcctgg
     1021 cgaagtcgtg atgctcagga tatagcaaat aaactgggga atatcagtaa ggctttcaaa
     1081 gctgccgatg taattatgaa gattgagaag gtcaaggaga aaagcatcga gggatatgaa
     1141 actggaaact gggggccgct gatgttggag gttgagtctt gggttctcag tggtatggcc
     1201 gcaagcgttg ccatgacatt gcttagtgct ataattggat cattcacact accggcgtta
     1261 gcattaactg caataaatat tgcaggtatt atgggaatta gttaccttgc atcatatatt
     1321 gatgcaagtg tggctgaaaa aataaataat aaattggttc attttactca ttgatagaaa
     1381 aagagaggtg atcatatcac ttctctttaa acgacctata ggaaccaata caaaaccaaa
     1441 aatacaaata tgagaatata aaaataatgg aatgtaagct aatgtaaaaa aacgatagga
     1501 ggattatatt tacagacatc agttttagca tttttgcaga actggttagc tccgtatcag
     1561 taaataaaaa agcatatatt aagatagcat aaaaaaaagt gaaaaaaata tttattttca
     1621 gtagttgcca tggagattta tcatttttta atctaagatt tttacatgtg tttaaaaata
     1681 aaagaatggc agtgaaagga gctgttttta catacagact caatgttttg ctaaatactg
     1741 gggtgttttg tgagtatatg gcaggtaggt catttgctct caatgctata tagtttaata
     1801 tattggatgt tggattgtgg atatatataa tgattattga gaacattggg attagtgaaa
     1861 ttataaggaa gtaaaacatc tttgttgcta ttttatttcc tttgtctgtt tggtggtgtt
     1921 tatttatcat ctgtttacct gtaaatttat tatattaaag attttgatga atggctatgg
     1981 tttattttct ctttagagag taatatatga aacttggcaa tcctactggg attgccatta
     2041 ataaata
//
LOCUS       DAZGXZ010000304         1985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000304 DAZGXZ010000000
VERSION     DAZGXZ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.306"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            166..360
                     /locus_tag="ACHKJF_005460"
     CDS             166..360
                     /locus_tag="ACHKJF_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952621.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGAWL"
     gene            357..1928
                     /locus_tag="ACHKJF_005461"
                     /pseudo
     CDS             357..1928
                     /locus_tag="ACHKJF_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head maturation protease, ClpP-related"
BASE COUNT          590 a          457 c          507 g          431 t
ORIGIN      
        1 ggatgacgag tcttaatact gcttcaatgg caaaaatgcg tcaataactt tgcctttctg
       61 agaataatca gaaaaatcat aatctgattt tatggtagaa aatgatttat ctattacttt
      121 tatcgatcaa taatgatgcc cgttaatcaa aacggaggcg gatttatgcc agagaacaac
      181 accagaaagc cggataaaag tgccacggta cacatagacg ccggaactat ggagaagatc
      241 gaacgctatc agcagttcat caaagataat cacccgggta tgccagtgcc cacgaaagga
      301 caaatcacac gcagcgcggt tgaatactgg tacagggcaa cgttaggagc ctggctatga
      361 aaacatggtt ttccattaag gctatggcag atgttgtaca tgtgcgcatt tatgacgaga
      421 tcggcgggta cggtgtaaag gcatcggcac ttactgacga gatcaacgcg tgcggtaatg
      481 tgtctgaaat ccatctgagc attcattcac ctgggggcga catctttgaa gggctggcta
      541 tctataacgc tctgaaaaat catccggcaa aaaaaatcgt acacattgaa ggcatggcgg
      601 cttctatggc gtcgtttatt gccatgtgtg gagatcacat cgttatgcct gaaaacgcga
      661 tgatgatgat acatgccccc cgtggtgtta ctgccggagt gtcgggcgac gttcgccgct
      721 ttgctgatct gatggacaag ctgggcgaca cgatggcaga aacctatgcc ggaagaacgg
      781 gcaggagcaa acaggagatc acctcaatga tggaggcgga aacctggatg gatggcaatg
      841 agtgtaaggc taacggcttc gcagatgagg ttatacccgc gattacagca atggcccgaa
      901 ttgaatcaaa acgaatcgga gatttttcaa atatgccgga aaaaattaaa agcatgatca
      961 gccagaaaac tggcagtggc gaacaggaac gacttaacgg aatccgtgaa ttgtttggca
     1021 ccttcaacgg aagatataac gacctggcta taagttgtct tgcggattca gaatgtagcg
     1081 ttgagaatgc acgcgaacgc cttttactcg ctatgggtaa agaatcaacg ccaacaaaca
     1141 aaaccacccc cgcaaatctt tactacgcgt acacggataa cggcaatata accggcgatg
     1201 ccatgcgtca ggggcttaat gcgcgtcttg gtcacgaacg ggccgaacgc ggtaatcctt
     1261 acgccatgat gagccttttc gatatggcac aggcatcatt aacccatcgt ggtataagca
     1321 cgggcagcta cagcacacgc tcgcagatag taaacgcggc attcacccac agcagcagcg
     1381 attttaccga tatccttgct ggtggcgctg aaaaatcagt gcttgcaggc tgggagcaca
     1441 gcggcgaaac attccgccag tggacgaaaa aaggttccct ttcaaacttc cgggaagccc
     1501 gccgcgttgg tatgaatggc ttctcaacgt taaacaaagt gccggaaggg gcagaatata
     1561 aatacatcac caccagcgat cgcggtgaac ccatcgcgct ggctacttac gggaatattt
     1621 tcagcattac ccgccaggcg ataatcaatg atgaccttga tcagttatca acggtgccaa
     1681 tggctatggg ccgtgcagca tcaagaacgg tgggaaatct ggttaatctg gtgcttacag
     1741 gcaacgtaaa actttctgac ggaataacgc tgtttgacaa aaaacacagc aacctgattg
     1801 aagcaggact gacaacaccg ggacttagtg cagcacgtca cctgatgcgc acacagaagg
     1861 acaaaaatgg cgaagtgctg aatattgcgc ctaaattcct tttagttccg gcagcactgg
     1921 aagatcgcgg tatggtcaaa tcaggcggga cagattcact ctgacaacaa ggcggcacca
     1981 gccgc
//
LOCUS       DAZGXZ010000305         1954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000305 DAZGXZ010000000
VERSION     DAZGXZ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.307"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHKJF_005462"
     CDS             complement(<1..209)
                     /locus_tag="ACHKJF_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HFP0952622.1"
                     /translation="MKKIIAVSALAVVGMFSGQALADRGKTGFYMTGKAGASVVTQTD
                     QRFCQDFGDDVYKYKGGDKNDTVFGA"
     gene            999..>1954
                     /gene="subA"
                     /locus_tag="ACHKJF_005463"
     CDS             999..>1954
                     /gene="subA"
                     /locus_tag="ACHKJF_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilase AB5 cytotoxin subunit A"
                     /protein_id="HFP0952623.1"
                     /translation="MLKNLRLHILFLLFISVSVRAEKPWYFDAIGLTETTMSLTDKNT
                     PVVVSVVDSGVAFVGGLSDSEFAKFSFTQDGSPFPVKEPEALYIHGTAMASLIASRHG
                     VYGVYPHALISSRRVIPDGVQDSWLRATESIMSNVFLAPGEEKIINISGGQKGISSAS
                     VWSELLSRMGRNNERLIVAAVGNDGADIRKLSAQQRIWPAAYHPVSSVNKKQDPVIRV
                     AALAQYRKGETPVLHGGGVTGSRFGNGWVDIAAPGQNITFLRPDGKTGIGSGTSEATA
                     IVSGVLAAMVSCNPRATATELKRTLLESADKYPSLADKVTEG"
BASE COUNT          541 a          435 c          469 g          509 t
ORIGIN      
        1 gcaccgaata ccgtatcgtt cttatcgccg cccttatact tataaacatc gtccccaaag
       61 tcctgacaaa agcgctggtc agtctgagtc acaaccgaag caccagcttt accggtcata
      121 taaaagcctg ttttgcctct atcagccagg gcctggcctg aaaacatgcc caccacagca
      181 agcgctgaaa ccgcaataat ctttttcatt tcagttatct ccgctatcac tttccccgaa
      241 cgataaacgt ggttgcttat tctaaagcta tgcaaaagac agggtaaaaa gagaaaaatc
      301 gatcgaatcg atcgacaaaa tcgatcatgc atgatgaaga tttcttatcg aatacataaa
      361 aatagtgatg gcaatcgtgg caggagctta cgtcaggccc tgaagtactg acacaggcat
      421 tgatggcagg tgatataagg agtgctccag ggagtgactc ccgactgacc caggcactgt
      481 cccgttgtca acccgtatgg ttactgctct gactcatttc ctgtcgtcag acgttcaact
      541 ctggaccact ggttatgacg acaggtgtcg ctcatttgtc agcactataa cggtcaaaga
      601 tcgtggcatt gacacattct ccagaggtaa atttctcaag aggtaacaac tctgccagga
      661 ggatatctgc agagagttac attcgtttgg gttcatatct gtgccgcgcg ttttccttcc
      721 atcagtgaac aaacattcag tcccatgacg accgggcaaa tagcgtgttc acagatattg
      781 ctgtccaggt acgaagtacc ataatggata ttggtaagtt attgggtgaa ccccctccat
      841 gaaaaaacca aatataaaaa tgaatgtttc gggagagact tcactcgctt tattgatagt
      901 gattgtttat cttttactga agacaggata tttcagatgt gatatccctg taacatattg
      961 accagcaata ctttttattt gtaccactgg aggatattgt gctgaaaaat ttacggctgc
     1021 atattctgtt cctgcttttt atctcagtgt cagtcagggc ggaaaaacca tggtattttg
     1081 atgcaattgg tctgacagaa acaaccatgt cgctcacaga caaaaatacc ccggttgtcg
     1141 tatctgttgt tgattccgga gtggcatttg taggaggcct cagcgacagc gaatttgcca
     1201 aattcagttt tactcaggat ggttcgccat tcccggtaaa agagcctgaa gctttatata
     1261 ttcatggtac tgccatggct tcactcattg cctcacgtca tggggtttat ggtgtttatc
     1321 ctcatgcact gatatccagc agaagggtta ttcctgacgg tgtacaggac tcatggctta
     1381 gagcaactga aagcattatg tcaaatgttt ttcttgcacc aggagaagag aaaatcatta
     1441 atatatcggg aggccagaag gggatatctt ccgcatcggt ctggtccgaa ctgctttccc
     1501 gtatgggccg gaataatgag cgattaattg ttgcggcagt gggtaatgat ggcgcagata
     1561 tacgaaaact gagtgctcag cagagaatat ggcctgcggc ttaccatccg gtcagcagtg
     1621 tgaataaaaa gcaggatcct gtgataagag tggcagctct ggcacagtac cggaaaggag
     1681 aaacacctgt attgcatggt ggaggggtca ccggaagtcg gttcggaaac ggctgggttg
     1741 atattgccgc accgggacag aatattacat tcctcagacc agatggaaaa acggggatcg
     1801 gtagtggaac ctcggaagca acagctatag tttccggcgt tctggcagcg atggtctcat
     1861 gtaatccccg tgcaacagcg acagaactga agcgtacact gctggagtct gcagacaaat
     1921 acccctcact tgcagataag gtgacggaag ggag
//
LOCUS       DAZGXZ010000306         1947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000306 DAZGXZ010000000
VERSION     DAZGXZ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.308"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1574
                     /locus_tag="ACHKJF_005464"
     CDS             <1..1574
                     /locus_tag="ACHKJF_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0952624.1"
                     /translation="VEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDA
                     QYYPKALAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKG
                     DTGERGPKGERGETGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDD
                     ETKAATPKAVKSAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWT
                     TSEFVAWLKEKGAFDQPYWMMKASLPAAMNKVITDVGPGKLNLGGCVIEVMGTYEAAI
                     VRVTIGEYGATGFINGTVCTCTVYGDTRYFHWRVDYSTKNKPDTVSQRDASTTQKGVV
                     QLSSDTGSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGP
                     KGETGATGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQ
                     TYAPQTDSTTWAVDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWRTVSVG
                     EYVSSGASGFEYFPL"
     gene            1616..>1947
                     /locus_tag="ACHKJF_005465"
     CDS             1616..>1947
                     /locus_tag="ACHKJF_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0952625.1"
                     /translation="MHIKNFRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVGQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARVMKGDE"
BASE COUNT          532 a          402 c          683 g          330 t
ORIGIN      
        1 atgttgagcc ggggttgtac cgggtgacgc tgaacacgga agggtacgcg ccgtcatatg
       61 tgggtgacat tctggtgaag gcagattctg caccgggaac gctgaataaa tttctgatgg
      121 acctggaaga cgcacagtat tacccgaaag cccttgcaga actggaagcg gtggcagcgg
      181 aaatcctgaa gcgtgcagaa gcgtcagcgg ccagtgcaga ggaagcgaag aaacgggcag
      241 agaatgcgcg gggaccgaag ggggataagg gagacaccgg gccacagggg attcccgggc
      301 caaaaggcga taccggcgag cgggggccaa agggtgagcg tggtgagaca ggaccacagg
      361 ggcttcaggg tgtgaaaggt gaacggggag agaagggcga aaaaggcgag ccgggaggac
      421 cggatgcgac gacggcacag aagggaattg tgcagttaag cagcgcaacg gacagtgatg
      481 atgaaacgaa ggcagccacc ccgaaagcgg tgaaatcggc gatggacaaa gcggacggat
      541 gcctggagaa agcgaaaaac ggtgacgata tcccggataa ggtgaagttt ctgaacaccg
      601 tgggagcagc cagagtatac gggcgggaca ttcatacggg ggccggtgaa tggaccacga
      661 gcgagtttgt tgcctggctg aaagaaaaag gggcatttga ccagccttac tggatgatga
      721 aggcatcact gcctgcggca atgaataaag tcatcacaga tgtcggaccg ggaaaactca
      781 atctgggtgg ctgtgtcatt gaggtgatgg gaacgtatga ggctgccata gtccgggtca
      841 ccattggcga atacggtgca acggggttta ttaatggcac ggtctgtacc tgtacggttt
      901 acggcgacac acggtatttc cactggcggg tggattacag cacaaaaaac aaaccggata
      961 cggtcagcca gcgggatgcc agcacgacgc agaaaggtgt ggtgcagtta agcagtgata
     1021 ctggcagtaa tgacgaaaca aaggcagcca cgccgaaggc cgtgaaggcg gcaatggatg
     1081 tggcaaatga agcgaaaaca aaggcagaag aggctgcagc aggaggtggt gttcccggtc
     1141 cgaaaggaga gaaaggcgac cccggagcac agggtccgaa aggtgaaacg ggagcaacag
     1201 gtcccgctgg tccgcgggga gcgcagggac cgaaagggga gaaaggcgac cccggtccca
     1261 gaggtgagcg tggagaaacc gggccgatgg ggccgcccgg ggcatcagac gggaagagcc
     1321 gggttgtggg tatcaggctg ggtaataaac agacttatgc gccgcaaaca gacagcacta
     1381 catgggcagt ggatttggat attggtgcaa tgattacggg gattggcggt tacaacgacg
     1441 gaaacaaaac ccttattgac agggtatctt acaggccgct tcaggtgaca tttgatggtt
     1501 ctcagtggcg tacagtcagc gtgggtgagt atgtgtcgtc aggagcttcg gggtttgagt
     1561 attttccatt ataaagcgca tcatctttca gacatgtaac cgggaggact tacagatgca
     1621 cataaagaat ttcagacaat acacgccgga aaatccggat gtgccgggtg cgatgtacct
     1681 gaaatcagag gatggtcagg actggtatga gtgtcagtcg ttattttcag cagagacgct
     1741 gaaggtggtt tataacagtg ccggggtcat taccggcatc agcagggtgg catcagttct
     1801 gtggccagtg ggccagagtg tggtggaggt ggcggatacg gaagaaaacc gcaaagctga
     1861 catctcgggg cgctggggtt ttgacgggga gaaaatcacg gacctgctga ccgcggagaa
     1921 agcgcgcgtg atgaagggag atgaaat
//
LOCUS       DAZGXZ010000307         1908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000307 DAZGXZ010000000
VERSION     DAZGXZ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.309"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            118..789
                     /gene="queE"
                     /locus_tag="ACHKJF_005466"
     CDS             118..789
                     /gene="queE"
                     /locus_tag="ACHKJF_005466"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP0952626.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   1129..1767
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1131..1157
                     /rpt_unit_seq="gttccccgcgccagcggggataaaccg"
BASE COUNT          511 a          446 c          473 g          478 t
ORIGIN      
        1 attaacgata tgtttcgcat ttttctttaa atctgcctgt aacagaatgt taataagaca
       61 tgattgttgt ggcaggatct gcaataatta acaattatcc acttcacaga gaatgctatg
      121 cagtacccga ttaacgagat gttccagacc ctgcaaggtg agggttactt taccggcgtc
      181 cccgccattt ttattcgttt acagggatgc ccggttggct gtgcctggtg cgacaccaaa
      241 cacacctggg aaaagcttga ggatcgggaa gtctcccttt tcagcattct ggcgaagacc
      301 aaagagagtg ataagtgggg ggcggcgagc agtgaagatt tgctggctgt cattggtcgg
      361 cagggatata ccgcgcggca tgtggtgatt acgggtggtg agccttgcat tcatgatttg
      421 ctgccactga ctgacctgct cgaaaagaac ggttttagct gccagatcga aaccagtgga
      481 actcatgaag tacgctgcac accgaatacc tgggttaccg tatcgccaaa gctgaacatg
      541 cgcggcggct atgacgtgtt gtcacaggca ctggagcgag ccaacgaaat caagcatcca
      601 gtggggcgcg tacgtgatat tgaagcactg gatgaactac tggcaacgct gaccgatgat
      661 aaaccgcgag tcattgcact gcagccgatt agccaaaagg atgatgccac acgtttgtgc
      721 attgaaacct gcattgcgcg taattggcgt ttgtcgatgc aaacacataa atatctaaat
      781 attgcctgat taaacattta taagcgttat aaatgggtgg aacctgtaaa gacttctact
      841 catttatatt gtttgtcgcc tctgaaaact cctccatttt acccatccag ggctaatcat
      901 tagcattctc tacaaattct gtggcattaa tttttcgctg gagtgaaaat tattgcggta
      961 aagtttggta gattttagtt tgtatagagt tattttaaat atttaccttt ttaatcaatg
     1021 gattaagtgc tctttaacat aatggatgtg ttgtttgtgt gatactataa agttggtaga
     1081 ttgtgactgg cttaaaaaat cattaattaa taataggtta tgtttagagt gttccccgcg
     1141 ccagcgggga taaaccgcgt agtgctgctg tatcgatgcc tgatgcgccg tgttccccgc
     1201 gccagcgggg ataaaccgaa ttcgggccac acgcgcgccc gttcatgcga gtgttccccg
     1261 cgccagcggg gataaaccgc gcaccgaggg ggaaaactcc ggcgcaggaa tgtgttcccc
     1321 gcgccagcgg ggataaaccg aacacctacg caaacgagca gtggctaaaa gggtgttccc
     1381 cgcgccagcg gggataaacc gatgtcgcca gaacaaaaac aaaaaacatt atagagttcc
     1441 ccgcgccagc ggggataaac cgccggtcac gtcaacgccg ataaacgtca tattgagttc
     1501 cccgcgccag cggggataaa ccgggtgatt ctcgcgacaa attgcgtaac cgggtgagtt
     1561 ccccgcgcca gcggggataa accgccctcc agtactgtga tgcgatcttt aagtccgagt
     1621 tccccgcgcc agcggggcta aaccgactgg ttctgtaacc caaatttggg atcattcgag
     1681 ttccccgcgc cagcggggat aaaccgaaac cggacgaggg cgcgctaaaa cgtgattaga
     1741 gttccccgcg ccagcgggga taaaccacca tataacccgt tatctctttc tcaagttttt
     1801 atattagcag tacttgtaat aagcaacata tccacgtaac acctcatgtt caaaatagtt
     1861 ctccatgcca gagaggttca caattatcga tacaaaaaat taaattta
//
LOCUS       DAZGXZ010000308         1886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000308 DAZGXZ010000000
VERSION     DAZGXZ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.310"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..446)
                     /locus_tag="ACHKJF_005467"
     CDS             complement(<1..446)
                     /locus_tag="ACHKJF_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFP0952627.1"
                     /translation="MSEVPLIAVVMAGGTGSRLWPLSREHYPKQFLQLSGESSLLQST
                     LLRLSTLSCETPLVITNEQHRFVVAEQLRQIDQLRDNIILEPCGRNTAPAIALSAFTA
                     LKRNEQQDPILLILAADHIISKADVFCDAIQKSKTVAEQGNIVTFG"
     gene            complement(439..939)
                     /gene="gmm"
                     /locus_tag="ACHKJF_005468"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(439..939)
                     /gene="gmm"
                     /locus_tag="ACHKJF_005468"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012565137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0952628.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASENVHANSRVYFNNDPRAIIGLNK
                     KEVKNV"
     gene            complement(942..>1886)
                     /gene="fcl"
                     /locus_tag="ACHKJF_005469"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(942..>1886)
                     /gene="fcl"
                     /locus_tag="ACHKJF_005469"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0952629.1"
                     /translation="IAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDSRAVHDFF
                     ASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKLLFLGSSC
                     IYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRSVMPTNLY
                     GPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMAAASIHVM
                     ELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASKPDGTPRK
                     LLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
BASE COUNT          453 a          508 c          464 g          461 t
ORIGIN      
        1 atcccaaagg ttacaatatt cccctgttca gctacagttt tcgatttttg aatagcgtca
       61 caaaaaacat ccgctttgct tataatatgg tcagctgcga gaatcaacaa aataggatcc
      121 tgttgttcat tacgcttcaa agctgtaaat gctgaaagag ctatggcggg agccgtattt
      181 cttccacatg gctcaagaat aatattatct cttaactgat caatttgacg taattgttct
      241 gcaaccacaa agcgatgttg ctcatttgtt attactaatg gagtttcgca ggaaagagtt
      301 gacagtcgta ataaagttga ttgcaataaa gaactctcac ctgatagttg caaaaattgc
      361 ttaggatagt gttctctaga cagaggccaa aggcggctcc ccgtaccacc agccattacg
      421 acagcaatca aaggtacttc agacattttt gacttccttt ttatttaagc caatgattgc
      481 acgtggatca ttattaaaat aaacacggct gttagcgtga acattttcac tggcgagcag
      541 tgcgtccggc gtcagccagc ggtaatcgtc atgctgctca tccggcagta acagctcttc
      601 ttccgctact ctgaagcgaa aaccgagcac cacatagtga gtggtgaaat ccgtgccgga
      661 aaagttatcg tcataaaagt gctgccagac accgtaaaac tggcctgctg ttatcggcag
      721 acgcagcccc agttccgcca tcgtcagccg ctcaaatgcg gcctccagcg tttcgtcttt
      781 ctgcacgcgc cctcccggca caaaccagta accctgcgcc gggcggttgg ttcttttgcc
      841 aagcagaaac tcgccgcgac tgttctcgac aataaagtcg agagagacaa gtggtgtgga
      901 gcgcactacc gtggcaaagt cttcctgacg taaaaacatc attacccccg aaagcggtct
      961 tgattctcaa ggaaccactg gtaagtgctg gcaagccccg cttccagtga gatttcgtga
     1021 taccagccaa gctgatgcag gcgcgtcaca tccagcagtt tgcgcggcgt gccatccggt
     1081 ttactggcat caaaaaccac ccgacctttg taacccacca ctttggcgat ggtttgcgcc
     1141 agctcgcgga tagtgcagtc aacgcccgta ccaacgttaa tgtgcgacag catcggctgg
     1201 gtgttctcca gccagacttc atgcgccagc tccatcacgt gaatgctcgc cgccgccata
     1261 tcatcgacgt gcaggaattc acgcatcggt gtaccgctgc cccataccac cacgtccggt
     1321 gcattctgtg ccgtcgcctc gtggaagcga cgcagcaatg cagggatcac atgcgaatta
     1381 ctcgggtgga agttgtcatg cgggccatac aggttggtcg gcatgactga gcggtaatcg
     1441 cgtccgtact ggcggttgta agattcgcac agtttgatcc cggcgatttt ggcaatggca
     1501 taaggctcgt tggtcggctc cagcgtgccc tgcaataact cgctttctgc catcggctgt
     1561 tttgccagtt tcgggtagat acaggacgat ccgagaaaca gcagtttgtt cacgtcgttc
     1621 tgatgcgcgg cgtgaatgat gttgctctca atcatcatgt tctggtagat gaaatccgcc
     1681 ggatacgtgt tgttggcaac aatgccgccc actttcgccg ccgccagata gacctggtca
     1741 atacgttcgc tggcaaagaa atcatgcacg gcgcggctgt ccagcaggtt cagttcgtcg
     1801 cgggtgcgta ataccagttc cacatcaccg cgctgttcga gctgccgcct gatggcggaa
     1861 ccgaccatcc cgcgatggcc agcaat
//
LOCUS       DAZGXZ010000309         1874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000309 DAZGXZ010000000
VERSION     DAZGXZ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.311"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            457..665
                     /locus_tag="ACHKJF_005470"
                     /pseudo
     CDS             457..665
                     /locus_tag="ACHKJF_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            652..841
                     /gene="tnpB"
                     /locus_tag="ACHKJF_005471"
                     /pseudo
     CDS             652..841
                     /gene="tnpB"
                     /locus_tag="ACHKJF_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(844..1032)
                     /locus_tag="ACHKJF_005472"
     CDS             complement(844..1032)
                     /locus_tag="ACHKJF_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0952630.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
     gene            1567..1708
                     /locus_tag="ACHKJF_005473"
                     /pseudo
     CDS             1567..1708
                     /locus_tag="ACHKJF_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          507 a          453 c          394 g          520 t
ORIGIN      
        1 gcatgaaaag caagttttac caatcttgat tttcagtgtt aaataagatt aataactgta
       61 attctgcaaa aaaaagaaca ccatatgtag tatgactacg tattccactc tgttcgttca
      121 cggactggca tcgaagtctg aggtgtactg gcaatagcgg atacagtcat ttgttctttt
      181 tttaagcatt catccgatga tacttttcct gaatgctacc ggagtggtat tcccctgccg
      241 tgagggaggg cgctgacggt tataaaaatc ctgatatata cccattcccg tatgacagcg
      301 atggcgtcac atctctgttc taccatcttc acagaaatat ttcgttcatt agcattccgg
      361 tattcgtata accaccttcg gtggagaaag aataaacaat ccctggaatg cgatgagcgc
      421 ttgaccagaa aagcatcata ttgcttcatg gcgcatcagc gaccacagga tgccgcaaaa
      481 gccgaaaaac ggaccagagc gcctgcaatc attcctcgcc tcagtatctg ttgcccatgg
      541 agtggccgat aacagatcat tgtcggatga gccttatcct cttccttccc ggtggtatcc
      601 ggatgtgctg ccagccttct cagttaaccg atgttttccg ggctttttgc catactgagg
      661 actgataacg tattcctggt cgtaaaaccc gtcaatatgc gccggggcat cgacaaaact
      721 gacacagtac gtccggaaag aacttaacgc tgcatggcac gacaatgctg cctttgtctt
      781 taccaacaag gctcgttccc gcataaaagt tctacgccgg gataaacatg gcgtatggct
      841 gcgtcaggcc ttcactactt cacaccggaa gtcttccacc ctgacacgaa tataccgtcc
      901 gggctctttc gcaggtgcaa acagtttttc acattcggcg aatgtcattt ctgaatcctg
      961 gtaacgcaac cactgaaccg gcatattacc gggtttgatt attgtcgcag ttattttata
     1021 tcgtttcccc atatcgcctc tcatcctgac aacaaaatta ccggctgagc catcactccg
     1081 gtaaagcatg cgaaaaaaag tgagcgtctc agcaatggct catgcgatga ttctgtacgg
     1141 gcaccgatgg cacctcagaa tacaacaaag atatcagagt aacatgtctc tctcgttatt
     1201 tttcagagtt cttgacaccc tccttctgcc acattgtcaa cgccagtgga gacttctgct
     1261 gtcagttttt tcatccatac agagtgacat taattatcct gaacaaaacg aaatatcctg
     1321 aatatattta ttttctcctg gtgccggaga catgatgatg tacaggctca ggtgcggtgt
     1381 gaatgtaaaa tggtatgtta attattcatt tctgaatgca aactgaatgc aaacttattc
     1441 cgaattgaat tctcctgaca ccacatgata aaaatgagga tataataaaa tacttctctt
     1501 ctggatgtgc cattacctgc cgttgctgta atacggcggg tattttttac tgacatatcg
     1561 ggaagccagc agacgttcgt agctacatcc ccgaaagtgg cacactgcaa actataatac
     1621 gagtaaaaca tcgatcaccg tagctgatgt gttcaacgat cgtgcactag cgttctgcga
     1681 ttctcaggga ctgcctgttc tgagaatcgg cgagtccgcg atcggatacg atgcgatttc
     1741 gcagtgactc agggattgca tctgaaaacc ctccggctat gtcggtaaat atttatttct
     1801 atgaaccgta cgattcagat agcattcgcc cggccccgta agaataatct ccggacaaat
     1861 aagatttttc ctga
//
LOCUS       DAZGXZ010000310         1829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000310 DAZGXZ010000000
VERSION     DAZGXZ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.312"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..709
                     /locus_tag="ACHKJF_005474"
     CDS             <1..709
                     /locus_tag="ACHKJF_005474"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0952631.1"
                     /translation="LVLSVTLSALPGKASTVSAEIPYQTFRDFAENKGVFTPGVTGIE
                     IKDNNGNAVGTLDVPMIDFSSVSRRGSLTLLSQGYGVSAKHGDLGDVNNASFGYDKNN
                     YTVVKNNKHSGLDFSLHRFSKLIAEATPADINISGQLSDSSQYTAFYRAGAGTQYIKE
                     RSGKQTHIPGTFLTGGTVGTPWYSGNNLISSSPGDTYNKSQGPLASYGQMGDSGSPLF
                     AYDSLSEKMVSGWSHPA"
     gene            633..1376
                     /locus_tag="ACHKJF_005475"
     CDS             633..1376
                     /locus_tag="ACHKJF_005475"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0952632.1"
                     /translation="MTAGRHYLLMILSVKKWSLAGVTLHNYGVNGYRNNWLLLPEDYI
                     RNIIVADFDPIISFNKNSKEHMSWTYDAAKGVGRIQQDDQQFVMHGNLNGNLNAGKNL
                     YFTGENGIIDLKDNVNQGAGYLQFADDYTVTTSNDSSWSGGGIIVNYGTTVKWGINGV
                     SGDDLHKVGDGTLIINGTGKNEGGLKIGAGTVILEQKAKNNDSTAFSSINISGGNSRV
                     KLSGDNQIIPDNVSWGFRGGIFRYKRKRH"
     gene            1321..>1829
                     /locus_tag="ACHKJF_005476"
     CDS             1321..>1829
                     /locus_tag="ACHKJF_005476"
                     /inference="COORDINATES: protein motif:HMM:NF014452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0952633.1"
                     /translation="MFPGDSGGGYLDINGKDTEFSRLQAVDYGAAIINSSTDKSLLTL
                     NLSPLKKDEIAVSVKALDMNAIFQGGHGTAGDLYKTTFYGPTQYYLLKKPKFGSVLMG
                     SLKNTSEWQFAGTDLNQAVDMAKNNKLTSSAQASYLYHGKLLGNMDIVIPELTGNDIL
                     TLDGSVSISG"
BASE COUNT          569 a          331 c          435 g          494 t
ORIGIN      
        1 cctggtatta tcagtgactc tttctgcttt accgggtaaa gcatcaacgg tcagtgcaga
       61 aataccatat cagacttttc gtgactttgc tgaaaataaa ggtgtgttta cccccggagt
      121 gacaggaatt gaaataaaag ataataatgg aaatgcagtt ggtacgcttg atgtgccaat
      181 gattgacttc agcagcgtgt cacggcgggg ctcactgacc ctgttgtcgc agggatatgg
      241 tgtatcagca aaacacggag accttggtga tgtaaacaat gccagctttg gctatgataa
      301 aaataattat actgtagtaa agaacaacaa acattcgggc cttgattttt ctctgcatcg
      361 gttcagcaaa ctcatcgcag aggctacacc tgctgatata aatatatctg gacaacttag
      421 tgattcgtca cagtatactg ctttttacag agcaggcgct ggcacacagt atattaaaga
      481 acgctcaggt aaacagactc atatcccggg aacattttta actggtggaa cagtaggcac
      541 tccatggtat tcaggaaata atttaatatc aagctctcca ggtgacacct ataataaatc
      601 acagggacca ttagccagtt atgggcagat gggtgacagc gggtcgccat tatttgctta
      661 tgattctctc agtgaaaaaa tggtctctgg ctggagtcac cctgcataac tatggggtga
      721 acggttacag aaataactgg ttgttattac ctgaggatta tatcagaaat attattgtag
      781 ccgatttcga tcccattatt tcatttaata aaaacagtaa agaacatatg tcatggacat
      841 atgatgcagc caaaggcgtg ggccgtattc agcaggatga ccaacagttt gtgatgcatg
      901 gtaacctgaa tggtaatctg aatgcaggta aaaatcttta ttttacaggc gaaaatggca
      961 ttattgattt aaaagataat gttaatcagg gggccggata tcttcagttt gctgatgatt
     1021 atacagtaac aacgagcaat gacagttcat ggtccggagg aggcattatt gtcaattatg
     1081 gtacaactgt aaaatggggt ataaacgggg tttccggaga tgaccttcat aaagttgggg
     1141 acggaacgct tattattaat ggaaccggga aaaatgaagg gggactaaag attggggctg
     1201 gtactgtaat ccttgaacaa aaagcgaaaa acaacgattc aacggctttt tcttccatta
     1261 acatcagtgg gggaaactcg cgcgttaaac tttcaggaga taatcagatt atacctgata
     1321 atgtttcctg gggattccgg ggggggatat ttagatataa acggaaaaga cactgagttc
     1381 tcccggttac aggctgttga ttatggggct gcaattatta acagcagcac agacaagtca
     1441 ttattgacgt taaatctgtc accattgaaa aaagatgaaa tagctgtaag cgtgaaggcg
     1501 ttagatatga acgccatctt tcagggggga cacgggacag ccggagattt gtataaaaca
     1561 actttttacg gtccgacaca gtattatctg ctgaaaaagc ccaaatttgg cagtgtgctg
     1621 atggggtcac tgaaaaacac cagcgaatgg caatttgcag gaacagacct gaatcaggcc
     1681 gttgatatgg caaagaataa taaactgaca tcaagtgcac aggcttcata tctttatcac
     1741 ggtaagctcc tggggaatat ggatattgtc attccggagc ttacaggcaa tgatatttta
     1801 acgcttgatg gctctgtcag tatatccgg
//
LOCUS       DAZGXZ010000311         1823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000311 DAZGXZ010000000
VERSION     DAZGXZ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.313"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..847
                     /locus_tag="ACHKJF_005477"
     CDS             <1..847
                     /locus_tag="ACHKJF_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0952634.1"
                     /translation="IENVFNLLDSLYVESVDTLTFKYQSIIKGLDRLFEGINLDSAFS
                     LSEEERSYFEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGFS
                     LALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFSE
                     EFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDTD
                     DIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYSEPDEGDNYLIKDGANFVIQF
                     NGVEF"
     gene            849..>1823
                     /locus_tag="ACHKJF_005478"
     CDS             849..>1823
                     /locus_tag="ACHKJF_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFP0952635.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKSDLIIVDYHLDNNAPD
                     KTLKLLQDLKDSDHLNMIVIYTRENLETVWMQISSTLKGALDINSLIIDYDNEDVQSY
                     WEDVVLPNLNDNGNKALTRDETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMI
                     AEYAVSRNAIISSNTSGNVIRGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTL
                     NDSLIEWKPSYYQLIKSEIQNAIEAEALSFVNHLANDHYGQAAWLNEIL"
BASE COUNT          622 a          276 c          348 g          577 t
ORIGIN      
        1 aattgaaaat gtgtttaatt tgctcgatag tttatatgtt gagtcagttg ataccctaac
       61 tttcaaatat caatcaataa taaaaggtct cgatagatta tttgaaggta taaacttaga
      121 ttcagcattc tcattatctg aagaagaacg ctcatatttt gaagaaaaag ctaaaagttt
      181 aaacgcgctt gcacagttag gtattagtgt tgagataata tctcatgaac ttgaagaaat
      241 ggattctatg gtaaccagag gactaaactc tcttcctact tctgtaaaag aacaccctgg
      301 tttttcattg gcgttaaatg ctcacagatc gcttactcaa caaatacgtt tcttatcacc
      361 tttgaaaata tcaggttatc aatccaggca gagaataact ggaaaaaata tcatggatta
      421 tgtcctgaag ttctttgggg agcgtttcga acggcaacga ataactattg aatttagtga
      481 agagtttaag caaatcgcaa taacagatat accatcaagg atctatcctg tttttactaa
      541 tattatcaac aatgcaatgt attgggtcag tctgtcaaat aataggctca taaagattgg
      601 ttttgtgaat tctttggtta tcatagcaaa ttctggtccg gcaattgata ccgatgatat
      661 cccgcgacta tttgaactat tttatagcaa aagagcaaat ggacatgggg taggtctgta
      721 tctatgtcga gaaaaccttg ctgttgcaca tcataaaata tggtattcag aacctgatga
      781 aggcgataac tatttaataa aagatggcgc taattttgtg atccagttca atggagtgga
      841 gttctaatat gacagtggca aattataatt ctcttgtcca gaaaactttc tgcgaaaatg
      901 caattcgttc cgttgtcatg attgatgacg attttctgac gtattctgaa tcaatcaggg
      961 cgttgaataa cgaagttgat ttagactaca acaaaattga ctcatctaaa cgagccgcta
     1021 ctcttgagag cttttttcaa tctaaaaata tgatttgtga tgttgacaat ggttctgtta
     1081 atttcgatgt ggatcggatt agaaaatcag atcttattat tgtagattat catcttgata
     1141 ataatgcacc tgataaaaca cttaaactat tacaagattt gaaagactcc gatcatttaa
     1201 atatgattgt aatatatact agagagaatt tagaaacggt ttggatgcag atatcatcga
     1261 ctctcaaagg tgctctggat atcaacagct tgatcattga ctacgataat gaagatgtcc
     1321 aaagttattg ggaagacgtt gtattaccga acttaaatga taatggtaat aaagctctca
     1381 caagagatga aacaatagcc tatattaaag acagtaagcc ttgtagaaga attaaaagat
     1441 taatacatga tgatgctgtg ttggaggatc aaaaggataa aaacttcatt gcaaaaatga
     1501 ttgcagaata tgctgtgtct agaaatgcaa ttatttctag caacacatct ggcaatgtca
     1561 ttcgggggga tgaaagcgga gtaaaatgga ttcaatgtgg taatatcttt gtctccctat
     1621 ttcataaggt tcaagatgat catgaaaacg atggagatag gatttggcaa actctcaatg
     1681 attctctcat tgaatggaaa ccatcttatt atcagttaat aaaatctgaa attcagaatg
     1741 caatcgaagc tgaggcttta tcttttgtaa atcatttggc taacgatcat tacggtcaag
     1801 ctgcgtggtt aaatgagata tta
//
LOCUS       DAZGXZ010000312         1796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000312 DAZGXZ010000000
VERSION     DAZGXZ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.314"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..338)
                     /locus_tag="ACHKJF_005479"
     CDS             complement(<1..338)
                     /locus_tag="ACHKJF_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP0952636.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTIIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFK"
     gene            complement(347..1114)
                     /gene="sctT"
                     /locus_tag="ACHKJF_005480"
     CDS             complement(347..1114)
                     /gene="sctT"
                     /locus_tag="ACHKJF_005480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFP0952637.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALFQHYHYELSTLNEIDIALFAAREIIIGLFIACLLSSPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTNGGLNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGFLARYASQLNAFAISLTV
                     KSALAFLILIIYFGPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(1116..1376)
                     /locus_tag="ACHKJF_005481"
     CDS             complement(1116..1376)
                     /locus_tag="ACHKJF_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0952638.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQRVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(1386..>1796)
                     /locus_tag="ACHKJF_005482"
     CDS             complement(1386..>1796)
                     /locus_tag="ACHKJF_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0952639.1"
                     /translation="ASVVNFVETGMSGYKSYLIKYSEPELVSFFEKIQKVNSSEDNEE
                     IIDDDNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLTLGMMMMSPVI
                     ISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          629 a          365 c          300 g          502 t
ORIGIN      
        1 aacttaaatt taacagcttc agttgctaat tgcattttac tttgaaacca ggagacaagt
       61 attgttgtga acatgcaaac cagcaaaaaa ggaatgatcg ttttcaacca accaaccccc
      121 atagctttaa aatagtccca aatatctggt ttagcgttat gatcaaggat gtattcaaga
      181 atcgacataa tgtgatgcac atcaaaaaca tatccgagat ataaagtacc cacaagcatt
      241 attacagaga tggttaagtc tctacttttt agaatttgcc cttttttaga agcatcctgg
      301 agtttctttt gtgttggctt ttcagtttta ttggccatga aacactctat ttctcaatgt
      361 atagttgcaa ttgttctggg aaaaaggata acggcattac tcgttcagcc agtattgggc
      421 caaaataaat aattaatatt aaaaatgcca gcgcactttt gacagtcagt gaaatggcaa
      481 aagcatttag ttgtgaagca taacgtgcca gaaatcccag gacagcctca ccgcccagca
      541 taacggcaat cacggggctt gcgtaaacga tagtatgagt cattatgtta ttaatatatg
      601 agaataaagg ctctaattta ggaaaattaa aattgcctga aggccataaa ttataacttt
      661 gccaaagagt ttccagaata aagttcaatc caccgttagt aagataaacc gctgcagaga
      721 ataggttaaa taatcttgct aattccgaag tatcgacacc agtagcagga tccaaagtac
      781 tacttaacgt tgcaccacgt tggttatcga taaaactacc tatagcaagg aaaatccaaa
      841 acggggatga taataagcat gcaataaaca gccctatgat aatttctctg gcagcaaaca
      901 aagcgatatc gatttcattt aaggtcgata attcatagtg gtaatgctga aataatgcac
      961 atgataccac tattattatt ggatgacgga ctactgttgg tatattccca ctggcgaaaa
     1021 atggtaacaa atagaaagtt ggtgccaatc ggcaaaaacc gagcgctgta gcagccagca
     1081 gactatgcaa ctgatagagt atcgcctccc ccatcctatc ctctcgcgaa tgctaaattc
     1141 agcatttcga taccaaacga atataatttt tcaccatacc atccgctcat taaaaaaaag
     1201 caaatactta cgcataataa tttgacgcca aatggcaacg tctgttcttg taactgtgtt
     1261 accctttgaa aaagccctac caataaacca acaaatgtcg caactgcgat aggtcctgca
     1321 gacataacta aaatcagata cagcgcccta ttccctgcaa aaacaatatc gtccatattt
     1381 tttcctcagg gattaatgga taggtcaaaa tattgtaaaa tgagtccttt tgataacatt
     1441 gtccaaccgt ccatagcgac aaatagaatt aattttatcg gtgtagatat gattaccggg
     1501 ctcatcatca tcatacctaa cgttaacaga acgctggata taaccagatc cactacgaca
     1561 aagggtaaat agatataaaa acctataata aatgcacttt ttatctcact caatgcatat
     1621 gcgggcagta acgagaaaat tgaaatatta tcatcatcga ttatctcttc attgtcttca
     1681 gaactattca ccttctgaat cttttcaaaa aagctaacca actcaggttc agaatatttt
     1741 atcaaatatg atttataacc actcatgcct gtctcaacaa aattaacaac cgacgc
//
LOCUS       DAZGXZ010000313         1791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000313 DAZGXZ010000000
VERSION     DAZGXZ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.315"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..241
                     /locus_tag="ACHKJF_005483"
     CDS             <1..241
                     /locus_tag="ACHKJF_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0952640.1"
                     /translation="LKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLID
                     DEQFDEINIVRGQPVSGGRLGVKIYPIMLEGQVKK"
     gene            238..1059
                     /locus_tag="ACHKJF_005484"
     CDS             238..1059
                     /locus_tag="ACHKJF_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0952641.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESEKQGVPVFRNCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
     gene            1285..1482
                     /locus_tag="ACHKJF_005485"
     CDS             1285..1482
                     /locus_tag="ACHKJF_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0952642.1"
                     /translation="MLIQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1633..>1791
                     /locus_tag="ACHKJF_005486"
     CDS             1633..>1791
                     /locus_tag="ACHKJF_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0952643.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGD"
BASE COUNT          488 a          369 c          497 g          437 t
ORIGIN      
        1 actgaaatta agcctgtccg gacggctggc aataaaaatt attgcagagc caccggataa
       61 gcgtcgtcgt gacctggaca acattctgaa agcaccgctg gatgcgctga cgcatgcggg
      121 actgcttatc gacgatgaac agtttgatga aatcaatatt gtacgtggtc agccagtatc
      181 tggtggacgt ctgggggtga agatttaccc cataatgctt gaagggcagg tcaaaaaatg
      241 aaactggaag atttaccgaa atactactcc ccaaaatccc ccggcctgac tgatgcatcg
      301 gcctcaacgt caaaagatgc gctgagtatc actgatgtga tggccgcgca gggcatgaca
      361 cagaatcggg ctgagatggg gttttctgcg ttccttggga aaatgggcat tagtatgaat
      421 gacagagagc gggcaacaga attgctgaca gaatatgcac tcagtcggtg tgatcgcgtg
      481 gcggcgttaa gaaaactccc ggcagaaata aaaccggcag tgatgcgtat tatggcttcg
      541 tatgcgtttg aagattatgc ccgtagcgcg gcgagcaaaa aacagtgccc ctgctgtcac
      601 ggaaaaaaat ttattgaaag cgaggttttt acaaacaaga tccagtatcc ggatggtaag
      661 ccgccagtgt gggcaaagtg cacaaaaggc gtgtatccgt cttactggga ggaatggaaa
      721 aaagtcaggg aggtggtaaa agttgcctgt ccggagtgtg gcggaaaggg ggaggtttcc
      781 accgcctgta aagattgtcg tgggcgcggt gttgccattc atcgtgaaga gtcggaaaaa
      841 cagggtgtgc cggttttcag aaactgccag cgttgtggtg ggcgtggcta tgaaagatta
      901 ccttcaacgg aggcatttaa tgccatatgt aatgtaaccg atgccatatc tcttgataca
      961 tggaaaaaaa cagttaaacg tttttacgat acgctggtgg tgcagtttga tattgaagaa
     1021 gcatgggcag aacaacaact gaaaaaggtg accagatagc tttgttgatt tttcccgaat
     1081 ctgtggtaaa tttgccctaa tgatgggcgt tttatgcctg acgttagaag atttttacac
     1141 ccgtcgccag gcgggttttt ttatgactga aatcacgcca gtacagtaaa cgcgctggtg
     1201 gcggtgaata ccggtctttc agcttgctgg cttttttgac aagagttatt ggtgtgtcac
     1261 gttaaccgga aaagggaaaa agacatgctg atacagcagg atatgaccga aaccgccaga
     1321 gtggtgttta atgaattaag cgtcaccgaa ccggcgacag tcggggagat tgcgcagaat
     1381 acttaccttt cacgcgaacg ctgccagtta atactgaccc agcttgttat ggcgggtctg
     1441 gcagactatc agttcggttg ttacagacgc cttcagtcat gaaggctttt ttatttgtgg
     1501 taaatgggcg gctggtgggt gttaggggca ctcaccagcc atctgctcat gcgtctggat
     1561 cacaagcaaa cctcaggccc actgctttgc gcaaaagcag aatgagccta tcagagacag
     1621 gcttaatgat ccatgcttaa tactgtaaaa atatccagtt gtgagttaat caacgccgac
     1681 tgcctggaat ttatccggtc gttacccgaa aattctgttg acctgatagt cacggatccg
     1741 ccgtacttta aagtgaagcc tgagggctgg gataaccagt ggaagggcga c
//
LOCUS       DAZGXZ010000314         1790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000314 DAZGXZ010000000
VERSION     DAZGXZ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.316"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..481
                     /locus_tag="ACHKJF_005487"
     CDS             <1..481
                     /locus_tag="ACHKJF_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0952644.1"
                     /translation="RDYSTHSWYVGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAW
                     KDQGMHLSMKDRDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRD
                     ASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            973..1338
                     /locus_tag="ACHKJF_005488"
                     /pseudo
     CDS             973..1338
                     /locus_tag="ACHKJF_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1348..1539
                     /locus_tag="ACHKJF_005489"
     CDS             1348..1539
                     /locus_tag="ACHKJF_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952645.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFSAL
                     KKENVGEFALRGVCVPGGE"
     gene            1573..>1790
                     /locus_tag="ACHKJF_005490"
     CDS             1573..>1790
                     /locus_tag="ACHKJF_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFP0952646.1"
                     /translation="MAYHSPDVTLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRM"
BASE COUNT          473 a          455 c          449 g          413 t
ORIGIN      
        1 ccgtgattac agtacgcatt catggtatgt cggtgctgaa gcaggctacc gctgtcatgt
       61 cactgaggat acctggattg agccacaggc agaactggtt tacggtgctg tatccggtaa
      121 acagtttgca tggaaggacc aggggatgca tctgtccatg aaggacaggg actacaatcc
      181 gctgattggt cgtaccggtg tggatgtggg taaatccttc tcaggtaagg actggaaagt
      241 gacagcccgt gccggtctgg gctaccagtt cgacctgctg gctaacggcg agacagtatt
      301 gcgggacgca tcaggtgaaa aacgtatcaa aggtgaaaag gacagccgta tgctgatgtc
      361 cgttggcctg aatgcagaaa tcagggataa cgtccgcttt ggactggagt ttgagaaatc
      421 cgcctttggt aagtacaacg ttgataatgc tgtcaacgct aacttccgtt actcgttctg
      481 atactccgta ttcaaaaggg cctgagggcc ctttatgctc aggacctgcc gcccaggtat
      541 acatttccgg agtatcagtc gatttgttta cttaaccgcc atgaccaaag ccggcagtaa
      601 tgtcggcttt ttacgtccat atattccact catccggaac tgtgacacag aacctgaata
      661 caggattgat caccggtgcc cataaatggc acattttcag taagattctg aatgttacat
      721 tcccccttca tgtgtgacac agctgtcaca gagaacgaaa cggaaagcac atgaaaaaca
      781 taaaaactca caccggactc cttatccaca aagaacaaac acggcgcgtt cgtcttcatg
      841 aaacaccggc cgcctggtgc cacacacgcc gggaatgcta cagcaaaacg accggtcgtc
      901 gctgcggcag ccctgacagt ctcagcaggc tgattctgag cagtattcag cccatagaat
      961 gagataaccc gtatgaatga tgacagacag acgtctacgg tacccgatgt atggaacatt
     1021 tgctttctgg ctaacggcat gcccgacaca tggttttaca ccgatgacgc ctccgtcaca
     1081 aaagagtggg ggcgggaata ttgtgacaat gtggaagtgt tccctctgtg ctatatgcat
     1141 gacggtcccg ttttccgggg gcgctatatt gatgagctca gcgccgcacg gaacagaatg
     1201 ctccatatgc accacatact gccggaatga acccggtaca gagaaaattt tcatgacgca
     1261 gaatgtatcc gtgaaattca cgaagcgccc ctgcaggctc ccggcattct gcaaaaatac
     1321 aaacgcacaa aatcataagg ctgttctatg tggatactga ttttctggct ctcatccccc
     1381 tttaatgtct cctccggcga tgcccggcaa cccacactgg ccgttcacag tcaggagttc
     1441 cgtactgaaa aagcctgtcg ggatgccttc agcgcgctga aaaaagaaaa cgtgggagaa
     1501 ttcgctctcc ggggcgtctg cgtccccggc ggagaataaa ccggagatga tgattctcca
     1561 caggagtatt caatggcata ccattccccg gatgtcacac tgcattatcc gtatctgcgc
     1621 cggatgtcct gggcgcagat aacggcagca gctgaagatg cggaaataca tcatgattat
     1681 gcccgggcat taatattgtg gcagcatgca tatcatgctg ccacgctgac tctcaacaaa
     1741 aacctggcag ttgctaaaat cgacttctgt attaaacgta tccggatgca
//
LOCUS       DAZGXZ010000315         1752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000315 DAZGXZ010000000
VERSION     DAZGXZ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.317"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..231
                     /locus_tag="ACHKJF_005491"
     CDS             <1..231
                     /locus_tag="ACHKJF_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0952647.1"
                     /translation="AAKAGKLPEAFFWTDAENNDVEVTAEELIALSEAAEQAMFTKGM
                     EIHVRQRTMKKELEKLTSADEILAYRVGWGDP"
     gene            637..>1752
                     /locus_tag="ACHKJF_005492"
     CDS             637..>1752
                     /locus_tag="ACHKJF_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952648.1"
                     /translation="MSKKITKTLLSSSLVVGLLSVSSFTRASEYSYSALDVLGKHGST
                     YTIVYNSDSDTAYVKWKKVPSGAEGIKDGDIVQGLSKINKDGTVEVTTKDGTSTYRVY
                     DREFHNLMNSLVKQDKENTLGKMEGTQTDLVSDTDIRNLFGRIAAISEMETNVGLDEY
                     GRVATSAESIKERVSLQSYLATEAANSAIGLDKNGKLTLNETDIVTPLRINDGVVTIA
                     KDVEEQKEGLAIQKKGLSELSSRTDIIDAATAANTNKIDSRTTVGVNADGTLTSADGA
                     TKTIAVNDGLVALSGRTDRIDAAVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQ
                     IRENHEEMKRAAAQSAALAGLFQPYSVGKFNATAALGG"
BASE COUNT          533 a          316 c          455 g          448 t
ORIGIN      
        1 gcagcgaaag cggggaaact gccggaggcg tttttctgga cggatgcgga aaacaatgat
       61 gtggaagtga cagcagaaga gcttatagcg ctgagtgaag cggcagagca ggcgatgttc
      121 accaaaggga tggaaatcca cgtccggcag cgcaccatga agaaagagct ggaaaagctg
      181 accagtgcgg atgagatact ggcatacagg gttggctggg gtgatccgta atctggaaag
      241 gaggacactg cgtattttta tggaaggaga acgcccaata accaattatg ctccttcacg
      301 gtaatacaca ggaaaagtta cttaggtaaa tattacactt taactgaaca gttacttttt
      361 actgctattg agatttttct cataaattac agtgtaagca gatgtgacac agcatgccgg
      421 atgagagtga gttttttctg atgcctgttc cggtggtgta gtcgtggttc tgagtgacta
      481 caccaagaat aatgtaagat aaatcccaaa ttattaaatt aatttttgga tttattttcg
      541 gaatatcctg aatgaagccg taattaatca tatttcttta tgagtatgag gtgttgaggc
      601 tgatttgtat acagataatt taacaggata tccataatga gtaaaaaaat cacaaagaca
      661 cttctgtcat cttctctggt tgtaggtctg ttgagtgtta gttcttttac acgtgcaagt
      721 gaatatagct acagcgcctt ggatgtgtta ggtaaacacg gaagtactta caccattgtt
      781 tataatagtg attctgatac cgcttatgtt aaatggaaaa aagtacctag cggtgctgag
      841 gggattaaag atggagatat tgtacaagga ttatcaaaaa ttaataaaga tgggactgta
      901 gaagttacta ccaaggatgg cactagtaca tatcgtgtgt atgaccggga gtttcataac
      961 ctaatgaatt cactggtcaa gcaggataaa gaaaacactc ttggaaagat ggagggtact
     1021 caaacagacc ttgtatctga tactgatatt cggaatcttt ttggcagaat tgctgcgatc
     1081 tctgaaatgg aaactaatgt tggtctggat gaatatgggc gagtggcaac tagtgcagag
     1141 agtattaaag aacgcgtgtc tttgcagagt tatctggcta cagaggctgc aaatagcgca
     1201 attggcctag ataaaaacgg taagctaact ctgaatgaga cagatattgt aacgccgtta
     1261 cgtatcaatg acggcgttgt cactatagcg aaagatgtgg aagaacagaa agaagggctg
     1321 gcgatccaga agaaaggact gtctgagctt tcatcccgca cagatatcat tgatgcagca
     1381 acggctgcga atacgaataa aattgattcc cgaaccaccg ttggtgtgaa tgcggacgga
     1441 accctgacca gcgctgatgg cgctacaaaa acgattgctg ttaatgacgg tctcgttgct
     1501 ctgtccggca gaacagaccg tattgatgct gcagttggta gcgttgaccg ccgggttact
     1561 aaaaatacac aggctattca gtccaatact cgccagttgc aggaacataa tgcacgcctg
     1621 aacagccagc agcgtcaaat tcgtgagaat catgaagaga tgaagcgtgc agcagcacag
     1681 agtgcagcgc tggcggggct tttccagccg tacagtgtgg ggaaattcaa cgccactgct
     1741 gccctggggg gg
//
LOCUS       DAZGXZ010000316         1734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000316 DAZGXZ010000000
VERSION     DAZGXZ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.318"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..207)
                     /gene="gadE"
                     /locus_tag="ACHKJF_005493"
     CDS             complement(<1..207)
                     /gene="gadE"
                     /locus_tag="ACHKJF_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0952649.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERI"
     gene            complement(1006..1578)
                     /gene="hdeD"
                     /locus_tag="ACHKJF_005494"
     CDS             complement(1006..1578)
                     /gene="hdeD"
                     /locus_tag="ACHKJF_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP0952650.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRTHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT          576 a          367 c          306 g          485 t
ORIGIN      
        1 aattctctcg gcatctaatt tctccagaaa tttaatcgct tcttcatcaa ggatatgatt
       61 gtgataggta tcaatgataa ccttgaaggg tttattgcct acttttttga tctctgacag
      121 ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct gtaaaagaaa
      181 agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg tttcgaacat
      241 gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag taaacgccta
      301 taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata cttcagattt
      361 atccatcacg cactccatat attgatccta agtaagattc ctggttgtta tcaatttgta
      421 aatatcaaac aaaaagttaa cgtttgcgga ttactgccca agaataagtt atcaataacc
      481 agtttgtgat ctctgaagaa tattactaaa gttaaaatgt aatccgattt aaatatcgag
      541 tctccttgtt tcgacttaag ctggcaattg gattgccagc tttcttttaa cgcattgaaa
      601 ttacctgagt aaataacgca ttcatgtctt caggttttta tatagcgtct ggagacacgg
      661 atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt acttatttaa
      721 ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc acttttaaaa
      781 cattaaacct gactacggaa aatatcagcc atgataatgt ttgaaatggc taatttgcca
      841 tggagtgaaa aaaattagat gaaattcagt aggttgaaat gatcactagc aggtaattat
      901 ttcaatgata gtgcgcaatt gatctacaac actgcgtagc ggagagagta ttaatcggat
      961 catagtcaca tcaagtgact atgatccggg tgacaaccgg gataattatt gctgcttaac
     1021 gaacaaactg gcgaagctga acaggctggc ggcgctaaat atcagttcaa ttcccaccag
     1081 tgtggaaacc agcgttacag acaccatcgg cgttgcacca aggaatatcc aggcaatgac
     1141 gatatccagc acaccaataa cgagctgtag ccagctgcct ttcattgaac gctgacgata
     1201 ccaactcatc aggcgaataa cccctgcaac acagaacaaa ccggcaataa atgccgcaat
     1261 ggcaaaaatg cccagctccg gtgcgcggat gaagaaatag ccgatcaata aataggcgac
     1321 tgcgacgagg aaaccggata ataccggcca gaaattatga gtgcggttgc tgaataaccc
     1381 gacaataagc gcaatacccg agcagattaa taatgcaccc actactgtgc ttaaaatatc
     1441 gccagagacg aacgggaaac tgatacacag caacccgacg ataaacagca gcacggcaat
     1501 aaactggatt gctctgcgat gctttttcag catctccaga tcaaacttca aaattgttgc
     1561 cttatctata tataacatag aaccacccta taaaattaag aagaaaatcc cctgctatca
     1621 atctatgcca aaaacgcgtc taagaatgca gtcgatttaa taaaaatttc ctaattacag
     1681 tatctgatgc atctgtaact cattgtattg aaataaaaat atctgatttt gata
//
LOCUS       DAZGXZ010000317         1730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000317 DAZGXZ010000000
VERSION     DAZGXZ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.319"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1730
                     /gene="rhsB"
                     /locus_tag="ACHKJF_005495"
     CDS             <1..>1730
                     /gene="rhsB"
                     /locus_tag="ACHKJF_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFP0952651.1"
                     /translation="RGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEIRYRYD
                     SDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEHADGSV
                     TQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSAT
                     GPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTMTWSRY
                     GQLLTFTDCSGYQTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQGHETRY
                     EYNIAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLTSENGS
                     HTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRLTHRTV
                     NGETAEQWQYDERSWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQ
                     HETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETLR
                     SFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGR
                     LTGVHTTAANL"
BASE COUNT          424 a          533 c          519 g          254 t
ORIGIN      
        1 cgcggcgaac tggcggcagt gtatgaccgc agcaatacac aggtgcgcag ctttacttac
       61 gatgataaat accggggccg gatggtggcg caccgtcaca cgggccgacc ggaaatccgt
      121 taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg cttaagctac
      181 acgtatcagt atgagaaaga ccgcatcacc atcaccgaca gcctggaccg ccgtgaagtg
      241 ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca cgcggacggc
      301 agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca gggcacagac ggatgccgca
      361 ggcaggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg cataaccacg
      421 ccggatggca gggcatcggc gttttactat aaccaccaca accagttaac gtcagccacc
      481 gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct gattcaggaa
      541 actgcccctg acggcgatat cacccgctac cgttatgata atccacacag tgacctcccc
      601 tgcgcaacgg acgatgccac cggcagccgg aaaaccatga cgtggagccg ttacggtcag
      661 ttgctgacct tcactgactg ttccggctac cagacccgtt atgaccatga ccgcttcggg
      721 cagatgacgg cggtgcaccg cgaggaaggg ctgagccagt accgcgcata cgacagccgt
      781 ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga atacaacatc
      841 gccggtgacc tgaccaccgt cattgccccg gacggcagca gaaacgggac acagtacgat
      901 gcgtggggaa aggccatctg taccacgcag ggcgggctga cgcgcagtat ggaatacgat
      961 gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac cttccgttac
     1021 gatgtactcg accggctgat acaggaaacc ggttttgacg gccgcacaca gcgttatcac
     1081 cacgacctga ccggcaaact tatccgcagc gaggatgagg ggctggtcac ccactggcac
     1141 tatgacgaag cagaccgcct cacgcaccgc accgtgaatg gcgaaaccgc agagcagtgg
     1201 cagtatgacg aacgcagctg gctgacagac atcagccata tcagcgaagg gcaccgggtg
     1261 acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca gacggtgcat
     1321 cacccgcaga cggaagcact gctctggcag catgagacca gacatgcgta caacgcgcag
     1381 gggctggcga accgctgtat accggacagc ctgcccgccg tggaatggct gacctacggc
     1441 agcggttacc tggcaggcat gaaactcggc gacacaccgc tggtggagta cacccgcgac
     1501 cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg aactcaccac cgcttatacc
     1561 cctgccgggc agttacagag ccagcacctg aacagcctgc tgtctgaccg cgattacacc
     1621 tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg gagttacagc
     1681 tacagcacca ccggcaggct gaccggcgtt cacaccaccg cagcgaacct
//
LOCUS       DAZGXZ010000318         1691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000318 DAZGXZ010000000
VERSION     DAZGXZ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.320"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1691
                     /locus_tag="ACHKJF_005496"
     CDS             <1..>1691
                     /locus_tag="ACHKJF_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP0952652.1"
                     /translation="MGKRLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLS
                     QQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYS
                     FSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLKMDAFGCTSRGQAHR
                     AGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRILSMDAASRT
                     LTLDREVTLPEAGTSTVNLINGSGKPVRVDITAHPAPDRIQVSVLPDGVETYGVWGLS
                     LPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGATFEPLSGSLNSVIPPAV
                     QHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGNGENSRLVTSAITADTEHRF
                     SGLPLGEYTLTVRAINSYGQQGEPATTAFRINAPAAPASIELTPGYYQITAVPVLAVY
                     DPTVQFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGRDFWFYVRSVNLVG
                     KSAFVEASGRASNDAEGYLGLFREKIGKLHLAQGMWELIDNSQLDNEMAEMKTTIT"
BASE COUNT          355 a          463 c          564 g          309 t
ORIGIN      
        1 atgggaaaac gcctgggggc cgcggatgtg gacaagtggg cactgtatgc catcgggcag
       61 tactgcgacc agacggtccc ggatggtttc gggggaacag agccgcggat gactttcaat
      121 gcgtacctgt cacagcagcg taaggtctgg gatgttctgg gggatttctg ctcggcgatg
      181 cgctgtatgc cggtatggaa cggccagacg ctgacgttcg ttcaggaccg tccgtcggat
      241 gtggtgtggc cgtacaccaa cagcgatgtg gtggtggatg ataacggcgt ggggttccgc
      301 tacagcttca gtgccctgaa ggaccggcac acggcggtgg aggtgaatta cactgacccg
      361 cagaacggct ggcagacttc cacggaactg gtggaagacc cggacgccat cctgcgctac
      421 gggcgcaatc tgctgaagat ggatgcgttc ggctgtacca gccgcggtca ggcccaccgt
      481 gccggactgt gggtgataaa gacagaactg ctggaaacgc agacggtgga tttcacgctc
      541 gggtcacagg ggctgcggca cacgcccggt gacatcattg aaatctgtga taacgactac
      601 gccgggaccc tgaccggcgg acgcatcctg tccatggatg ccgccagccg taccctgacg
      661 ctggaccgtg aggtgacact gccggaagca gggacatcga cggtgaacct gattaacggc
      721 agcggtaagc cggtgcgcgt ggacatcacc gcacaccccg ccccggaccg gatacaggtc
      781 agcgtcctgc cggatggcgt ggagacatac ggtgtgtggg gactctccct gccgtcactg
      841 cgtcgtcgcc tgttccgctg tgtttccatc cgggaaaaca cggacggcac ctttgccatc
      901 acggcagtgc agcacgtacc ggaaaaagaa gccatcgtgg ataacggggc cacctttgag
      961 ccgctctccg gttcactgaa cagcgtcatc ccgccggctg tgcagcacct cacggtggag
     1021 gtgagtgcct cagacggcca gtatctggcg ctggcgaaat gggacacgcc gcgggtggtg
     1081 aagggcgtgc gcttcagtct gcgcctgacc agcggaaacg gtgaaaacag ccgcctggtg
     1141 accagcgcca tcactgccga cacggagcac cgtttcagtg gcctgcctct gggggaatac
     1201 accctgacgg tcagggcgat aaacagctac ggccagcagg gcgaacctgc caccaccgcg
     1261 ttccggatta atgcgcctgc agcaccggcc agcattgagc tgacgccggg gtattatcag
     1321 ataacagcag taccggtgct ggcggtgtat gacccgacgg tacagtttga gttctggttc
     1381 tcggaggcaa aaatcgcaga cacatctcag gtggaaacct ctgcccgtta tctggggaca
     1441 ggcagccagt ggagtgtctc cggcccgcac attaagccgg ggagggattt ctggttttat
     1501 gtgcgcagcg tcaacctggt ggggaaatct gcgtttgtgg aagccagtgg ccgggcgagc
     1561 aatgatgcgg aagggtatct ggggctgttt cgggaaaaga taggaaaact gcatctggca
     1621 caggggatgt gggagctgat tgataacagc cagcttgaca atgagatggc ggagatgaag
     1681 accaccatca c
//
LOCUS       DAZGXZ010000319         1655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000319 DAZGXZ010000000
VERSION     DAZGXZ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.321"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(35..766)
                     /locus_tag="ACHKJF_005497"
     CDS             complement(35..766)
                     /locus_tag="ACHKJF_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0952653.1"
                     /translation="MTIPLLEINSLSFSYKVNLPPVFNNLSLKIEQGELIGLLGENGA
                     GKTTLFNLIRGGVSNYEGTLKRNFSGGELVSLPQVINLSGTLRNEEVLDLICCFNKLT
                     KKQAWTELNHKWNDNFFIRYDKIRRKRTYTVSYGEKRWLIISLMLTLCKNARLFLLDE
                     PTVGIDIQYRMMLWELINKITADGKTVFFSTHIFDELTRDKIPFYMLSKNSINRYSDM
                     SDFIQSNNETTPEKAFIKEVMGTGD"
     gene            complement(763..1257)
                     /gene="mcbE"
                     /locus_tag="ACHKJF_005498"
                     /pseudo
     CDS             complement(763..1257)
                     /gene="mcbE"
                     /locus_tag="ACHKJF_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258040.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="microcin B17 immunity protein McbE"
     gene            complement(1223..1534)
                     /locus_tag="ACHKJF_005499"
     CDS             complement(1223..1534)
                     /locus_tag="ACHKJF_005499"
                     /inference="COORDINATES: protein motif:HMM:TIGR04424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McbB family protein"
                     /protein_id="HFP0952654.1"
                     /translation="MINILPFEIISRNTKTLLITYISSVDITHEGMKKVLESLRSKQG
                     IISEYLLDKLLDESLIDKDKGKEFLITTGVINKTKTSPLWVNSVIISDKRKWFYCYLR
                     A"
     gene            complement(1593..>1655)
                     /locus_tag="ACHKJF_005500"
     CDS             complement(1593..>1655)
                     /locus_tag="ACHKJF_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin microcin"
                     /protein_id="HFP0952655.1"
                     /translation="GCSNGCSGGNGGSGGSGSHI"
BASE COUNT          512 a          312 c          281 g          550 t
ORIGIN      
        1 gttttgtgat tcttttttct attatatcca tgtgtcagtc tcctgttccc ataacttctt
       61 taataaatgc cttttctgga gttgtttcat tattggattg aataaaatca ctcatatcag
      121 aataacgatt tatactattt tttgaaagca tatagaaggg gattttatct cttgttaatt
      181 catcaaaaat atgagttgag aaaaataccg ttttcccgtc agcggtgatc ttatttatca
      241 gttcccataa catcattctg tactgaatat caatgcctac agttggttca tccagtaaaa
      301 ataaacgggc atttttgcaa agcgttagca tcaaagatat gataagccaa cgcttctctc
      361 cataacttac cgtgtaagtc cttttacgtc ttattttatc atagcgaatg aaaaaattat
      421 cattccattt atgattaagc tctgtccatg cctgtttttt agttaactta ttgaagcagc
      481 aaatcaaatc aagaacctcc tcattcctta gtgtacctga aagatttatg acttgaggta
      541 gacttactaa ttctccacca gagaaatttc gcttgagggt cccttcataa ttactgactc
      601 cgcccctgat aaggttaaaa agagtggtct tcccggctcc attttctcca agcagaccta
      661 tcaactctcc ctgctcaatc ttaagcgaca gattattgaa aacagggggt aaatttactt
      721 tatatgaaaa gctcagagag ttgatttcta aaagaggtat ggtcattgag aactccagac
      781 tggctcagtt ttgaaggtta gtgctgaaat aatacttatg attaacatta tactatagaa
      841 aaatatcgtc atcagagaca actcctggtc tgactggagt attgtagaat agatggcgat
      901 aggattaacc tgatttatcc agtaaacgat cccctcatta tattttaatg atacaattcc
      961 tgataccata cagacagtta ttaatacaga atagatagtg ctggctgttt ggaatgtgac
     1021 tctgaacgat gccatgaaag tgagtgaaac catcataaat gcatttatgt atatcgattt
     1081 taaaataatc attactattt gataatctgg acttgcctgg aatcctgtta gtacgactaa
     1141 aatgaaaaaa aacacataca aaatagacat gataagtgag gctattaatt gagaacgaat
     1201 aaataatagt cgaccatcca tattatgcac gaaggtagca ataaaaccac tttctcttat
     1261 cgcttattat gactgagttt acccagagag gtgatgtttt cgttttattt atcacgcctg
     1321 tggtaataag aaactctttg cccttgtctt tatcaataag cgattcgtcc agtagtttat
     1381 caaggaggta ttcagaaata ataccctgtt tggatcgtag gctttcgagt acttttttca
     1441 ttccctcatg tgtaatgtct actgaagaaa tgtaggtaat aagcagggtc tttgtatttc
     1501 tggaaattat ctcaaatggc agaatattga tcatatcttt tcctttttaa tatcagggag
     1561 caccatgctc cctgaacggt taattcaacg tatcagatat gtgaaccact tccgccgctg
     1621 ccaccgtttc caccactaca accgttgctg caacc
//
LOCUS       DAZGXZ010000320         1618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000320 DAZGXZ010000000
VERSION     DAZGXZ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.322"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            256..825
                     /locus_tag="ACHKJF_005501"
     CDS             256..825
                     /locus_tag="ACHKJF_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0952656.1"
                     /translation="MTYKYNPFWQRRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYAWAREFGVLRGKKHYHLLLLVN
                     RDTWCRAGDYRDPGSLAGMIKQAWCSALGVDAGPYATLAHFPDTPAVWLERGDDTGFQ
                     QVLERADYLAKEHTKAHGTGERNFGCSRS"
     gene            1084..1467
                     /locus_tag="ACHKJF_005502"
     CDS             1084..1467
                     /locus_tag="ACHKJF_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0952657.1"
                     /translation="MYAKSFIAFDGNGRLTGARTVQTAPYDRYTCHLCGSSLRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREIQLIKRLQKFVPDALPVVRKASWHCRQC
                     HHDYYGERYCTHCHTGHFSEEGVAE"
BASE COUNT          367 a          444 c          436 g          371 t
ORIGIN      
        1 gtattatgag taatcgcgtt tccacagtcc ctgcacttct ttttgcgtat caccataact
       61 ccctcctcac agccttaccc gtaaccggtt ttttacatta aaaaaacaac gctgaaaatt
      121 caaccgtcag tccggagacg accgttcggg ttatcacaga gtgcctgaga cagtgtcctg
      181 ccggaggtca cttcagggac tctgcgcatt ttttacgacg tggctattcc gtatgaacca
      241 tacggagatt taaccatgac ctacaaatac aaccccttct ggcagcgacg tattcgtgag
      301 acggtgcggc acgcgctgga tgttcatccc cgcctgacgg cacttcgggt tgatctgcgt
      361 ctcccggatg taccggcagc tacggacgcg gctgtgatat cccgcttcat caatgccctg
      421 aaagcccgaa tcgacgctta ccagaaacgt aagcatcggg aaggtaaacg cgtgcatccc
      481 accaccctgc attacgcctg ggcccgggag ttcggggtac tcaggggcaa aaaacactat
      541 cacctcctgc tgctggtcaa ccgggatacc tggtgtcgtg ccggcgatta ccgcgatccg
      601 gggtcactgg ccgggatgat taaacaggcc tggtgcagtg ccctgggggt ggatgccggg
      661 ccttatgcca cgttggcgca ttttcctgac acgccggcgg tgtggctgga acgcggtgat
      721 gacactggct ttcagcaggt gctggaacgt gctgactatc tggcgaagga acataccaaa
      781 gctcacggca ccggtgagcg caattttggc tgtagccgga gttgaacacg acagacgcgc
      841 caatacccgg caaaccaaaa gtatgggttg ttgtctggcg gcaacgatgt atccactcgc
      901 ccactgactg ttttttacgt cagtgttcca ttgtgtgaca accacagtgc tgtaacagca
      961 accagcctgc tttacccaaa ctccagtcca gattttaact gaaccgccat tcacacctga
     1021 ccacgcagcc cgtaccggga cggctgacgc ctgtgtgtcg tctggcggtt gaggagtatg
     1081 attatgtacg caaaatcctt tattgcattc gatggcaacg gacgtctgac cggtgcccgt
     1141 accgtacaga ctgcgccgta tgatcgttat acctgtcatc tttgtggcag ttcgctcaga
     1201 taccacccgc aatacgacac tgaacgtccc tggtttgaac acactgacga cgggctgaca
     1261 gcgcacggtc agcagtgtcc ttatgtcaga ccggagcgca gagaaataca actgattaag
     1321 cgtctgcaga aattcgtacc ggatgcctta cccgtggtgc gtaaagccag ctggcactgc
     1381 agacaatgtc accacgatta ttatggggag cggtactgca ctcactgcca tacagggcac
     1441 ttcagtgagg agggagtggc tgaatgaacg gtataaaaac ggggctgggg atcacgccgg
     1501 gtgaacacat tatcagtgcg gcctcagccc ttagcagaaa tatcaggcat tgtttctgcc
     1561 tttcctgtcg tggtcggttg attttgcaga cagatgctca gggggcgtgg tttgagca
//
LOCUS       DAZGXZ010000321         1611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000321 DAZGXZ010000000
VERSION     DAZGXZ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.323"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1611
                     /gene="espI"
                     /locus_tag="ACHKJF_005503"
     CDS             <1..>1611
                     /gene="espI"
                     /locus_tag="ACHKJF_005503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_062895919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspI"
                     /protein_id="HFP0952658.1"
                     /translation="ALIFQGHPVIHAGQTVSASQSDWENREFSLNNLNLNNADFSLSR
                     NAFMNGNIRAVNQSTVIIGGDTVFTDKNDGTGNDVISVEGKSAAAGTSSYTGHITLEQ
                     KSALDIRDNFRGGVTSEDSHINVSSSSVLFSDASSFINSSLNIHKGGALTAQGGLFTS
                     GSIDIGDASLLLTGTPVNSDDAAFLPTINMADGGFKLMSDSSVLKARDQASVVGDIIS
                     DKQATISFGTESGKEGILSEKASRGLAVGLLSGFNTAYRGAIHAPSASATMNNTWWQL
                     TGDSSLRSLKNTGSMTYFTGSAANKAFHTLTVDELTTNGTAYAMRTDLKNADKLVVNQ
                     KLSGKDNILLVDFLNKPTGEKLDIELVSAPGNSSKDVFKGSEQAIGFSNVTPVITAKD
                     AGDKTTWNLTGYRMAENPAATQSASGLASVGYKSFLSEVNNLNKRMGDLRDINGEAGA
                     WARIMSGTGSAGGGFSDNHTHVQVGVDKKHELDGLDLFTGFTVTHTDSSASADAFKGK
                     TKSVGAGLYASAMFDSGAYIDLIGKYVHH"
BASE COUNT          450 a          354 c          403 g          404 t
ORIGIN      
        1 gctcttatct tccagggaca cccggttatt catgcagggc aaactgtttc tgcatcgcag
       61 agtgactggg agaacaggga gttctcactc aacaatctga atcttaataa tgcggacttc
      121 agtctgtccc gtaatgcatt tatgaacggg aatatcaggg ccgttaacca gagcactgtt
      181 attatcggcg gagatacagt ctttactgat aaaaatgacg gaacaggtaa tgatgtcatc
      241 agtgttgaag ggaaatctgc tgccgcagga acatcctcct atacagggca tatcactctg
      301 gagcaaaaat cagcactgga tatccgcgat aattttcgtg gcggggttac gtctgaagac
      361 agtcatatca atgtttcttc atcttcagtc ctgttctcag atgcatcgtc atttataaac
      421 agctccctga atattcataa aggaggtgcg ctgaccgctc agggagggct gtttacaagt
      481 ggaagcattg atattggtga cgcttccctt ctgcttaccg gtacaccagt gaattcagat
      541 gatgctgctt ttttaccgac catcaatatg gctgatggcg gatttaaact gatgtctgat
      601 tcatcagtac tgaaagccag agaccaggca tctgttgttg gtgatattat ttctgataaa
      661 caggccacaa tcagcttcgg aactgaatca ggtaaagagg gcatattgtc tgagaaggca
      721 tcccggggac tcgcggtagg attactgagt ggttttaata cggcataccg cggtgcaatt
      781 catgccccgt cagcatctgc cactatgaac aacacctggt ggcaactgac aggagactcc
      841 tcacttcgct cgttaaaaaa taccggaagc atgacatatt ttacaggaag tgcagcgaat
      901 aaagcattcc atacactgac ggttgatgag ctgacgacga atggcactgc gtatgccatg
      961 cgtacggacc tgaaaaatgc ggataagctg gtagtaaacc aaaagctgtc aggtaaggac
     1021 aatattctgc tggttgattt tctgaacaaa cccaccggag aaaaactgga tattgaactg
     1081 gtgagtgcac cggggaacag cagtaaggat gtttttaaag gaagtgaaca ggcaataggt
     1141 ttcagcaatg tcacacctgt tattacagct aaagacgccg gagataaaac aacatggaac
     1201 ctgaccgggt acaggatggc agaaaatccc gccgcaaccc aaagtgcctc aggccttgca
     1261 tctgtggggt acaaatcatt tttgagtgag gtcaacaacc tgaataaacg tatgggtgac
     1321 ctgcgtgaca tcaatggtga agctggcgca tgggcacgta tcatgagcgg aaccggctct
     1381 gccggtggtg gtttcagtga caaccacaca catgttcagg tcggtgtcga caaaaaacat
     1441 gagctggacg gactggattt gtttaccggc ttcactgtca cacacactga cagcagtgcc
     1501 tctgctgatg ctttcaaagg taaaacaaaa tctgtggggg ccggactcta tgcttccgcc
     1561 atgtttgatt ccggtgccta tatcgacctg attggtaagt atgttcatca t
//
LOCUS       DAZGXZ010000322         1573 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000322 DAZGXZ010000000
VERSION     DAZGXZ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.324"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1573
                     /locus_tag="ACHKJF_005504"
     CDS             <1..>1573
                     /locus_tag="ACHKJF_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0952659.1"
                     /translation="YMNLRKKHICIAISLVLNPFPASLAAQNTNVVEVDETDKGYQVY
                     GDNWKSIEIGTTKEVKKKNSTDLNFDHNSPVAKGHNSIAIGTSAEAGGEIKDEKGFKT
                     GESIAIGAKAKATKEQSIAIGGDTKATGWGAIVIGGDDLTPLHGDRFGDKEIPNNYSS
                     SEASGDGSIVVGSRSTAAGDLSVSLGSISSANGTAANALGATAEANGDMATAVGFNAL
                     ANASNALAVGSQSSASGEDSLAIGGQSTTGKKSTVALGQGATASAAEGNVSIGADSVD
                     KAATPVSGATIKLKNGGTIEYKGFAGNNAASVVSVGDTDKERQIVNVGAGAVSASSTD
                     AINGSQLYAIAKTLKDDLDAINPTWKLKDSTGKEKEVGSKNGGTTGNEVTVIGKGDID
                     VTVDGNGLSIDGTKLAENIKKDAIKIKTGDGQTLTKKLGEEVEFNDAGPIKTKINNGK
                     LEITAETASFTPDGNGGNGKITVSNGDDDKLVTAKKIADAINQSGWKIKADSENGGKV
                     EGDNKEELINPGDTVT"
BASE COUNT          508 a          280 c          430 g          355 t
ORIGIN      
        1 tatatatgaa cctcagaaaa aaacatatct gtatagccat atcacttgtt cttaatccgt
       61 tccctgcatc actggcagca caaaacacta atgtagttga agttgatgaa acagataaag
      121 gttaccaagt ttatggtgac aactggaaaa gtattgaaat aggtacaacg aaggaagtaa
      181 agaaaaagaa ttcaaccgac cttaattttg accataactc cccggtagcg aaagggcata
      241 actccattgc gattggtacc agtgcagaag caggaggtga aattaaagac gaaaaaggtt
      301 ttaagacagg agaatccata gctattggtg ccaaggcgaa agcaactaaa gaacagtcaa
      361 ttgcaatagg tggcgatacc aaagctaccg gatggggggc tattgttatt ggtggagatg
      421 atttaacccc tttgcatggt gacagattcg gtgataaaga aattccgaat aattactcgt
      481 catctgaagc atcaggagac ggaagtattg ttgtgggctc aaggagcaca gctgcaggag
      541 acctgtctgt gtccctgggc tcaataagct ctgccaacgg gacggctgct aatgcactgg
      601 gagccacggc ggaagctaac ggggatatgg cgactgcggt tggttttaat gcgctggcta
      661 atgccagtaa tgctctggct gttggtagtc agtcctctgc cagcggagag gattccctgg
      721 ctataggggg tcagagtacg acgggcaaaa agtccacggt tgctctgggg cagggagcta
      781 cagcctctgc cgcggagggg aacgtgtcca tcggtgctga ttcagtggac aaggcagcaa
      841 ctccggtttc cggcgctacc attaagttaa agaatggcgg gactattgaa tacaagggct
      901 ttgccggaaa taacgcagca tcagtagtat ctgttggtga taccgataaa gagcgtcaga
      961 ttgttaacgt gggggcgggt gctgtatctg cctcaagtac agatgccatt aacggtagtc
     1021 agttatatgc gatagcgaag acgctgaaag atgaccttga tgccattaat ccgacgtgga
     1081 agttaaaaga cagcacagga aaagaaaaag aggtcggcag taaaaatggc gggactacag
     1141 gtaatgaagt gaccgtcatc ggtaaagggg atattgatgt tactgttgat ggaaatgggc
     1201 tgagtattga tggcacaaag cttgctgaaa acatcaaaaa agatgccatc aagattaaaa
     1261 cgggagatgg tcaaacacta acgaaaaaac ttggtgaaga agttgagttt aatgatgccg
     1321 gaccaataaa aacaaaaatt aataatggca aacttgagat aactgcagaa acagccagtt
     1381 ttacgcctga tggaaatggt ggtaacggga agattacagt aagcaatggt gatgacgata
     1441 agcttgttac tgcgaaaaag attgctgatg ccataaacca gtctggctgg aaaattaaag
     1501 ctgacagtga aaatggtggg aaagttgagg gggataataa agaggagcta attaatcccg
     1561 gtgatacagt tac
//
LOCUS       DAZGXZ010000323         1572 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000323 DAZGXZ010000000
VERSION     DAZGXZ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.325"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(944..1420)
                     /locus_tag="ACHKJF_005505"
     CDS             complement(944..1420)
                     /locus_tag="ACHKJF_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0952660.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          361 a          416 c          389 g          406 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag ccttaccagc tgtgattttt gccagatttc
      181 acgctctcaa cgttcaacag ctccatcatg gttttgccgt ccgttcaccg ctaaaacgcc
      241 tcagaaatcg ggacaggatg tgcaaatgta ataccgtcac ccgcgacgat attccgtttg
      301 ccatctggtc agggcttcgc cccgacaccc cgcatcagtg cgtacgtgct caatgtggtt
      361 tccggttctc ttccgcttcc tcgcccactc gctcactaac gttcggtcgc tggggctgcg
      421 gcgagcggtg ccacctctcg catgttgtcg gtgattccgc tgttgttctt tcggggataa
      481 gtcgtcagat agcccttcaa acgcgctcag aagctctcag acgcacgaaa cggggtttaa
      541 atgcccgtca gggcatggaa ggcgcgaaaa acgcttacag agcgtttcag gaggtcaggc
      601 gcgccgatac ccctagccag gggctttcag gtcgccagga accgcgccag ccccctgcaa
      661 agcccatttg cacggtacag gcagccactc ggttggtagg tggtcgtaga ccgtcggtgt
      721 tacagcgatg ggggtctggg gttctcccca gacggtttcc ggttgcgctc aaggcgctgc
      781 cggaaccgtg gcggctcgat cgttctggag atcggggagc aaaaacacgc aagggatttt
      841 gaggcggcaa aagagcaatg caggatagac aacccgatgt caaatgttaa catttgaatc
      901 agattgtcca aaaacggggt ttttgcctgt gcctgccgct ggctcaattt gaaaacatgg
      961 ccatgattcg gcgctgaata tcaccgtctt tgttgaccag tgttttagtg acaaacgccg
     1021 cgtagttttt agatgtgttg tatcccttcc agacttcatg tggatcgagt gcatacgcac
     1081 aaaccttacc agcaacagga attctgacta acgcgttttt tgattctaag tagttgagcc
     1141 accgaataat tgtgcttctg ctaactccaa gtttttcagc taggaattgc tgatccgcaa
     1201 cgacagcgcc acacgtagga tcgatatgct ccgctaaaaa tgagtacagc ttcagtgctg
     1261 atatgccttg gctatccttc agcagctctc gaacacgttc ccaacctttt ggggatacct
     1321 gtgtaaacct tgaattcttc tttgcttctt cgagatcctc ggtgtttttc tttgcctcat
     1381 ctgaaagttg tttcagtcgt ttttctgaat caataaacat gattttttcc ctctgaaaat
     1441 cccatgcact aacgttggtt caaatctaag ccatcatgac acatctgtgc aacaccttgt
     1501 tgcgtgttgt cgcatccagg atgcgacaac gttgtgtcac cctggatgcg acaggtaaac
     1561 aaaaaacaca ct
//
LOCUS       DAZGXZ010000324         1571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000324 DAZGXZ010000000
VERSION     DAZGXZ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.326"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..185
                     /locus_tag="ACHKJF_005506"
     CDS             <1..185
                     /locus_tag="ACHKJF_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952661.1"
                     /translation="RIGRCMGYLQVAAEIKALASGADKDSVVREALLRDFDNPPFKKV
                     PAYWLHPGLTYLKVRI"
     gene            222..755
                     /locus_tag="ACHKJF_005507"
     CDS             222..755
                     /locus_tag="ACHKJF_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0952662.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEALRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1274..1459
                     /locus_tag="ACHKJF_005508"
     CDS             1274..1459
                     /locus_tag="ACHKJF_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0952663.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
BASE COUNT          384 a          357 c          456 g          374 t
ORIGIN      
        1 agcgtattgg tcgttgcatg gggtatctgc aggtagccgc tgagattaaa gcgctggcca
       61 gtggtgcgga caaggattca gttgtgcggg aggcacttct tcgtgatttt gataatccgc
      121 cttttaaaaa agtgccggct tactggcttc atcccggact gacttatctg aaagtgcgta
      181 tttagtgggc ctgggacagc ggctgaatat ttaatatatc catgaacaca aaaatcaaat
      241 acggcctgtc ggctgccgtt ctggcgctga ttgccgcagg ggcttctgcg cctgaaatcc
      301 tcgaccagtt tctggatgaa aaagaaggca accacaccac ggcataccgt gatggcgcgg
      361 gtatctggac catctgccgt ggagccaccc gggtggatgg taagcctgtt attcctggca
      421 tgaagctgtc gaaggggaaa tgcgaccggg ttaacgccat tgagcgtgat aaggcgctgg
      481 catgggtgga gaaaaacatc aaagtgccac tgactgaacc ccagaaagcg ggtattgcgt
      541 cattctgtcc ttacaacatt ggcccgggta agtgtttccc gtcgacgttt tataaacgaa
      601 ttaatgcagg agatcgcagg ggagcgtgtg aggcgcttcg ctggtggatt aaggacggtg
      661 gcagagactg ccgtatccgc tcaaataatt gctatggtca ggtatcccgt cgtgaccagg
      721 agagcgcgct ggcgtgctgg ggtatcgaca gataagcaga atattttgct gaaaaatgac
      781 gttggccaac gcggacggat aacacgaaat cctgcgaact ggcaaaacct aagtgaataa
      841 aagtaaaaac cccgtttgtt agcagcaggt ggggttttgt gtttcctgac tccggaaaag
      901 tcaaaggaga aagtgtgttt gattttagca aactgattcg ggagatttga gtgatggctg
      961 aaaaattatc tacctggaag ttcattctta tctggctggt gtttgtgatt atggtctccg
     1021 gttatttcat cggtcagata cgctggtggt gaaatgaacc gcgttctgtg tgtggtcatc
     1081 attgccctgc tggtggcctg tggtgcgctt agtctggggc tgaatcatta ccgtgataac
     1141 gccatcacct acaaagcgca gcgcgataaa aaagccagag agctggagct ggcaaacgca
     1201 accattactg atatgcaggt gcgccagcgc gatgttgctg cgctcgatgc aaaatactcg
     1261 agggaattag ctgatgcgag agctgaaaat gaaactctgc gtgctgatgt tgccgctggt
     1321 cgtaagcgcc tgcggatcaa cgccacctgc tccggtaccg tgcgtgaagc caccggcacc
     1381 tccggcgtgg ataatgcaac cggcccccga ctggcagaca ccgctgaacg ggattatttc
     1441 atcctcagag aacggttgat gacaatgcag aagcagctgg aaggggcaca ggactatatc
     1501 cgcactcagt gcctgaacta agttttgcgg atgcgccgta tcgtcgctgt attccctcat
     1561 taacagagac c
//
LOCUS       DAZGXZ010000325         1562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000325 DAZGXZ010000000
VERSION     DAZGXZ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.327"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..424)
                     /gene="sctJ"
                     /locus_tag="ACHKJF_005509"
     CDS             complement(<1..424)
                     /gene="sctJ"
                     /locus_tag="ACHKJF_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
                     /protein_id="HFP0952664.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTA"
     gene            complement(421..752)
                     /gene="sctI"
                     /locus_tag="ACHKJF_005510"
                     /pseudo
     CDS             complement(421..752)
                     /gene="sctI"
                     /locus_tag="ACHKJF_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(772..1012)
                     /locus_tag="ACHKJF_005511"
                     /pseudo
     CDS             complement(772..1012)
                     /locus_tag="ACHKJF_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1026..>1562)
                     /locus_tag="ACHKJF_005512"
     CDS             complement(1026..>1562)
                     /locus_tag="ACHKJF_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0952665.1"
                     /translation="KNQPGKPVRVINPDDESIRIFSWLADNFPDLQYFKLQLLDASNL
                     RLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQAIETLSAIGLSY
                     EKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFS
                     YGGNRFEKSQGSNWTFQE"
BASE COUNT          455 a          317 c          258 g          532 t
ORIGIN      
        1 aagcagtttt accactatcg ccattatcaa catcgtaact aacatgtact ctcgaagaaa
       61 taatgccatc cataattttg agagattgct ctaaccgctg ctcaatagca gaatatagcc
      121 ttgctttttc cgctcgtgga gatgaaacga gtgcatctgc agggaacatc tgcgatattt
      181 gaatatcagg cttacccggt agattgtaga tttttagcca atccaccgca gaagcaaaat
      241 ccgttggttc gacaaatatt gaaaatcctg ttttgccttg atccttcttt tcagcattaa
      301 tattatgtct ttgtaaaaca gcaaggacat cattagcttg ctgttgatca agatggttca
      361 ataattcctg ctgcttgcag ccgcacaaca gcaggataaa caataataat gatatatatt
      421 tcatgagcgg gtcaacgtct caactgcagc gacaatcttt cttgctaatg ttccaccaat
      481 gaaacctcga cattataatt aagcacatga tcctgcaaga ctaataattt ttcaggagtt
      541 aacccctctt tatcagacct taatgcttgt aacgcatttt gctgccaatt ttcagtttgt
      601 acagcatagt tggcaaaagc ggagatcatt ttatcatccg ggttgtcaag attgagaacc
      661 cggtcattta aatcaatcac tttcgcttgc tgtatttcat gagcagttga ttgctcagct
      721 ctatcaatag gtggcatatt gcttactgac ataataaaat ccttatccag tttagcggaa
      781 gttttgtatg attgctgaat caagatcttt atacgctttt acaacgttag attgcgcatt
      841 tcgatataat gtatactcag ctaatgcact ttgatattcg gcaagaaatt tcgtgtcgga
      901 gggattggtt ggctaaatcc tccaacgctt tttcaacttg ctggttcaga tcatcaacgc
      961 cctgatcaaa ttgattgctg atatccataa tataaccatt ccaatctgcc ataagtcctc
     1021 ctcaattatt cctgaaatgt ccagttgcta ccttgagact tctcgaatcg gttacctccg
     1081 tagctaaatg aactattatt tgtgtcctga tttttcaaat tcacattaaa tcttacatat
     1141 tgtttacccc attgtttata atattcgtca acatagttat taattttatt taattcactg
     1201 tcacttaacg taccaatgat attgaaatac acattgttag ctgttttata tttttcataa
     1261 gaaagaccta tcgctgacaa cgtttcaata gcctgacttt ccaatacatt atcatctaat
     1321 accgcaatac ttatattgct ggcatatggc atagtttgca gtaatccttt aataagatta
     1381 tcgattagtt gctgtgtgat agcgttccgt tgcttactca ctgtcaatct tagattactg
     1441 gcatctaata gttgaagttt aaaatattgt aaatcaggga aattatcagc aagccatgaa
     1501 aaaattcgta tcgattcatc atcaggattt atcacccgta ctggctttcc tggttgattt
     1561 tt
//
LOCUS       DAZGXZ010000326         1536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000326 DAZGXZ010000000
VERSION     DAZGXZ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.328"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..490)
                     /locus_tag="ACHKJF_005513"
     CDS             complement(<1..490)
                     /locus_tag="ACHKJF_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP0952666.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNGSNFHSQQLNLSFHEKINKYGYNSPIRIIKECIENGIN
                     STP"
     gene            671..823
                     /locus_tag="ACHKJF_005514"
                     /pseudo
     CDS             671..823
                     /locus_tag="ACHKJF_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          491 a          224 c          285 g          536 t
ORIGIN      
        1 agggagttga attaatgcca ttttcgatac attctttgat tattcgtatt ggactattat
       61 aaccatattt atttattttt tcatgaaaag ataaatttaa ttgttggcta tgaaaattgc
      121 tgccatttcc atatctaaaa tgttcaatca gatcttcgat aagtgtttta tattgtccaa
      181 aaaaggaaag catttttgct agttctttca tttctgcaaa taaaatatca gtgcattcac
      241 gttgtgatat ttttgttccg gaacttgagg cactaaaagc aacattaata gaacctgggc
      301 cagggaaatg gtatttaata agtctgtaag gatcaacttt tgctgagata tcgcttaggc
      361 cgagtgagag aaagtcttgt tctaccatat caccatattg catatcatca gcagaaaaat
      421 catcgaataa actaatagtt tcgaaaagtg tacagggaat attaattggc ggaagtaata
      481 tgttattcat ttgcatttgc cttggttata tttaagctca atatcaattc gagaacaaat
      541 gctagggtaa aaaaatattt aaaaaagaag taaacttcac attttttcat tacatctctg
      601 aagagaacct cgttaatgat tggtcttgct cattgttaat aacaaaatgt tgaatttacc
      661 gattaggaaa ctttttccta cgcggattgc ctccagctat ggcatgaact ttgagtttat
      721 gccagaactg aagtggggct tcggctaccc tggcgcgatt atctttatga tcctcgcggg
      781 cctgacatcg tatctgtact ttaagcgtaa gaactggctg taactatacg atgtcgctgt
      841 gagtaagtta gtgtgctgac agcgacattt tagccgaggg aatcgcagat aacataattg
      901 ttgtgaataa tatccatgcc acgttgagtt ggacgcttaa tgactcacat ctcacccctt
      961 caattgctgt agagataata gaaaaaagtg tcaaacccaa tgaaatcaat acaaagtaga
     1021 ttgccttaca tccagtatct cttattcttt ttgctccaat attgatattt acataggtat
     1081 tattaataac cagaataaaa ctcaaagcca ggttgatatc attcaaagtt attaatattg
     1141 accatgatgt taatgtatag attatgaact gaaagcgtga taaacgctca agcctggatg
     1201 ggtagaatat atttctgatg gatgatttga acatttttac attgttatat atataattgt
     1261 tttgaatata tattacctga attatgtggt ttgcatatat atgcaatata gcagtctttg
     1321 cattgataac tatacagtag taatttagag tgtgtaagtt tttattgatg gggtatattt
     1381 taattaaata taattgaaat gtatttttat tctataaaca atatcttggg tgaatggatt
     1441 ctatatggga aatggtaact tttggcagtt gttcttgggt aaagaacctt acagcaaaaa
     1501 atcttatttt gttaaggcat ggttattgtg gtgggc
//
LOCUS       DAZGXZ010000327         1518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000327 DAZGXZ010000000
VERSION     DAZGXZ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.329"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..616)
                     /locus_tag="ACHKJF_005515"
                     /pseudo
     CDS             complement(1..616)
                     /locus_tag="ACHKJF_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
     gene            complement(780..875)
                     /gene="ymiC"
                     /locus_tag="ACHKJF_005516"
     CDS             complement(780..875)
                     /gene="ymiC"
                     /locus_tag="ACHKJF_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP0952667.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(989..1156)
                     /gene="yciX"
                     /locus_tag="ACHKJF_005517"
     CDS             complement(989..1156)
                     /gene="yciX"
                     /locus_tag="ACHKJF_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP0952668.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1159..1299)
                     /locus_tag="ACHKJF_005518"
     CDS             complement(1159..1299)
                     /locus_tag="ACHKJF_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP0952669.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          418 a          357 c          346 g          397 t
ORIGIN      
        1 ccgttgatca tagtggtgtg cgagtcagta ccaacgagtg tatccggata agcaatccat
       61 tcaccgtcct gcaattcact ccacactgct ttgccgagat attcgaggtt aacctgatgg
      121 caaatgcctg tgcctggcgg cacgacgcta aaccgactga acgcttgctt tccccatttc
      181 aggaacacat aacgttcgtg gttgcgctcc atttccaggc gtacgttttc ttcaaatgcc
      241 tcattatcac caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc gaccggtgag
      301 agcgggttaa cctttgcagt atcgccgccg aggcgtttaa ccgcttcgcg cattgccgcc
      361 agatcaacaa cggcaggtac gccggtaaag tcctgcatca gcacccttgc cgggcggtag
      421 gcaatttcac ggtcagcatg ggcatttttc agccatcctg ccagcgcagc aggttttcga
      481 gcaaaacttt gagtgacttg ggtagacggg tgatatcacc cagtgattta gcagcaagcg
      541 gcaggctgta gtagtggtaa gttttatctt tggcctgcaa cgtgtcctta ctggcttctc
      601 gtagggttga cgacatagct cctccttaat gacagggttg cgaagccctg attctcttca
      661 gggtgaatat taaagataac acaaacatcg cgtaacgatt tgataacaac ccaaattgat
      721 aaaagaggag aaaccttgtg gcaaagcaaa tgacaacccc gccgcagcgg ggtcaaggat
      781 cagtgagtaa tgatccagag gagttcggcc cagaagagca tcgacagaga aaacgcgccc
      841 atccaggacc aatatttcat atttgtgttg atcataggct acacccttaa cggttattat
      901 tttgtgttga gataactcaa gctgttattg ctgcggtgta ttaaaagccc tttaattcat
      961 taaaaatggg taataagaat gatgataatt attgtttcgg ctgatcatct tcagcaaaca
     1021 gttctataaa ggcttgctgc tgcgatgtta gttgccagtc gtctggaata acgacggaac
     1081 attcccgttt cgtttctgtt tcattgtcac tatgctggca taatggtgac gactccagtt
     1141 gctcctgacc gaccatagtt atccccacac tttccaaatc agcagtgcga caaccaccca
     1201 gaaaagtgca gaaccaagaa aaaccgccag ccaggcttta cgttttaatt caggatcgcg
     1261 gcggggttcc tgatttccag aaggcattgc taacctcata taatcgccat cacttatcag
     1321 caagacgttg aacgattggt gtaattaatc atacgatgag acaaatccta aagaaacttt
     1381 agtcaaaaat ccagcgaatt tacgaatcgt ctccagtgaa taattcaatc ttttgacctg
     1441 aaatagatat ttgaaaactg gagtttgcga ggcgggtaat tagacactac tttacaggta
     1501 atttacccta taaatatg
//
LOCUS       DAZGXZ010000328         1510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000328 DAZGXZ010000000
VERSION     DAZGXZ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.330"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(157..810)
                     /gene="ribB"
                     /locus_tag="ACHKJF_005519"
     CDS             complement(157..810)
                     /gene="ribB"
                     /locus_tag="ACHKJF_005519"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP0952670.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(914..1062)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1184..1474
                     /gene="ubiK"
                     /locus_tag="ACHKJF_005520"
     CDS             1184..1474
                     /gene="ubiK"
                     /locus_tag="ACHKJF_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP0952671.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          429 a          387 c          345 g          349 t
ORIGIN      
        1 tgattttgat cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag
       61 aagcggaatt aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc
      121 ggtttcttga ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc
      181 ctgacggtat gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa
      241 ctcaatgcac tctggtgcac gcgccatcgt gccatcgtca ttagtcaact cacacagcac
      301 accagccggt ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc
      361 acgcgtcagt acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag
      421 atcagaaggt tttgcgccat cggcaatcgc tgcgcgaacg gttgtaatac gatcagcggc
      481 agaaacaccg gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata
      541 ggcgctggtg ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt
      601 gatgcacagg caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt
      661 catggtttct gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac
      721 cattacaccg cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt
      781 accaaaagag gaaagtagcg tctgattcat ggtaaaaaaa cctcactaaa attatggtta
      841 ccagaatcag ggcagtctta ggagtggcgg catatagcca aaataacgtg agcgggtcca
      901 tgcccgacag aatcgttact ctctcccatc cggactctaa ccgtcggccc cggaattaca
      961 ccggatctgc tgtcctttga gttcgcaccc aaagcgctcg cgggctttca actgagttga
     1021 tttaccgccg gtggggaatt tcgccccgcc ctgagaataa gcggattcac tataacgcta
     1081 atgattagcg gcagcaacgc atagcttcac ataattctgg tttatgactt acccttatcg
     1141 cactacaatg gcactcaaca cctatcagta cagggaaact acaatgattg acccgaaaaa
     1201 aattgagcaa atcgctcgcc aggttcacga atcaatgcct aaaggaatca gggagttcgg
     1261 ggaagatgtg gagaaaaaaa tccgccaaac cctacaagct cagctgacgc gcctcgacct
     1321 tgtaagccgt gaagagttcg acgtccaaac gcaagtcctg ttacggactc gtgagaaact
     1381 ggcgctgctt gaacaaagaa tgagcgaact ggaaaatcgc agtactgaga ttaaaaagca
     1441 gccagatcca gagactctcc ccccaacgct gtaaacattt gtctttaatg cactgtaaat
     1501 tatgaattaa
//
LOCUS       DAZGXZ010000329         1509 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000329 DAZGXZ010000000
VERSION     DAZGXZ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.331"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1187)
                     /gene="rhsD"
                     /locus_tag="ACHKJF_005521"
     CDS             complement(<1..1187)
                     /gene="rhsD"
                     /locus_tag="ACHKJF_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFP0952672.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPDPLPFILSRTYSSYRTKTPAPVGIFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRELTGLADRFGR
                     TLTYRREAAGDLTGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     AFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPAAPLVRYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQDRIG
                     QPEP"
BASE COUNT          318 a          467 c          399 g          325 t
ORIGIN      
        1 ggttcaggct gtccgatgcg atcctgctca tactggtagc ggtaactcag gcctgccggg
       61 ttaagttgct ccaccacccg ccccgcatcg tcgtagcggt agcgcatctc cggcctgcct
      121 gcataacggt gcgccaccat ccggcccggg tgctgcgcgt catacgtgaa agcgcgcacc
      181 tgcgtattgc tgcggtcata taccgccagc agttcaccgg cttccgtata tgtgtaccgc
      241 accagtggcg cagcgggcag gctctccggg tatgccgggt cgtgcatcag ccacaccgcc
      301 gaaaggcgga tacccctgtc ggggccgtat tcggtaccgg gcagtgtgtc ggggaacgct
      361 gaggctgaga gagggcggga actgtcagaa gaagatagcg aagaggtgcg ggcctcttcc
      421 gcacgctgcg cctgcgtggt cagcaccaga cggaactccc gcccggcacc gtccgtcacg
      481 ccggtgattt ccccggtcag gtcaccggcg gcctcacgcc ggtacgtcag cgtccgcccg
      541 aagcggtccg ccagccccgt cagctcccgg tacggcggca gcggcgctgg cagcacgtcc
      601 tcagcacccg gcacccgctc tgaccagccg agtatccacc acggcccctg tgcgctgttg
      661 gtcgccaggt aaagatgcgg gcttaaccgg atatccggcg gcagcgcccc ccacagccgc
      721 gccagcgtat ggccgtccgg ctgtgctgcc ttaccaccgc gcaccagcca cattgactca
      781 ctgcggctgt acaccgcctc ccccggcagc agcggctcaa agtgaatgct ccgcccgccg
      841 ttgtcgttga gtaccagtgc atcatcgcgt atctgtaagc ggatatcaga aggcgctttc
      901 cagccggggc caaaaattcc caccggcgca ggcgtcttcg tccggtagct gctgtaggtg
      961 cgggagagaa tgaacggcag cgggtcgggc agcgcaaggt ccgtctcgcc gggctgtact
     1021 tttgcaccga gcagcgggtt caccgggttg cccgaggtca tcccgcccgg acacaccgag
     1081 cacgccacgc cagttggcgc gccaattctt acacctgccg aaccctggac aatgggaccg
     1141 ccatactgag tcatatctcc ctgacgcgcc gctggttttc cgctcatata aagtgccctt
     1201 ttagttattg ttcatatccc tgtgttagca acaaaaaaaa taaaatgaca cacaagacca
     1261 gctaatgtat ttatatattt ttcacataaa cttatttgtt ctcaatcaac aacaactgtt
     1321 attacaggtc actttcaata ttttattaaa ctaaaatttt tagcacagat gaattcatca
     1381 tcaacaaatt cactatatgg ataatatcca ttcaacgcat atgatttatg attttattag
     1441 tctgagttat ttttgtaggg ctatatttaa aattattaaa ccccaccgat tcagattaat
     1501 atcgcaata
//
LOCUS       DAZGXZ010000330         1502 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000330 DAZGXZ010000000
VERSION     DAZGXZ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.332"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..247
                     /locus_tag="ACHKJF_005522"
     CDS             <1..247
                     /locus_tag="ACHKJF_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0952673.1"
                     /translation="KGVNSFSDFYAIDLTSKNGRELCRTLAYKTFEKIIINTDNKSCD
                     IYFMNGIVFKHYPLMKVISAQQAISALKYMVDGEVYF"
     gene            359..682
                     /locus_tag="ACHKJF_005523"
     CDS             359..682
                     /locus_tag="ACHKJF_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFP0952674.1"
                     /translation="MDAGVLVLAVQQSPITKQFTDNELCTLAWLWRAGNVMLITYQNV
                     TPLLQVAEHREAGRFTSIEQEYPQILNKAQAILARETAHVKFRPWQDDKWSRVLPHLR
                     SDRLQ"
     gene            1144..1500
                     /locus_tag="ACHKJF_005524"
     CDS             1144..1500
                     /locus_tag="ACHKJF_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952675.1"
                     /translation="MDTIITWMGDRLLGWITNKSDLRQRAITGLTPAICSTILYTEKL
                     KRGEPNNPNEEEKLYRLWYEASSQVVDFDRELAKRCLDKSEYWLHSELYSPEKVGELN
                     ISLVGMKATLEGIKHN"
BASE COUNT          468 a          288 c          333 g          413 t
ORIGIN      
        1 taaaggtgta aattcattca gtgattttta tgctattgac ttaaccagta aaaatggacg
       61 agagttatgc cgtacacttg cctataaaac attcgaaaaa atcataatta atacggataa
      121 taaaagctgt gatatctatt ttatgaatgg cattgttttt aaacactatc ctttaatgaa
      181 agtaatatcc gcccagcagg cgataagtgc tctcaaatat atggttgatg gtgaggttta
      241 tttttgagta ataatcactt tttcaaccgt gctatagtaa gaaagttagg taagtacaat
      301 aaaattatct atcctgaacg aagcgtcctg agctatggtt ttactatagg gactgccaat
      361 ggatgctggc gttctcgttc tagcagttca acaatcccca atcacaaaac aattcactga
      421 taacgaactt tgcacactcg cctggttatg gcgagcaggg aatgtgatgt taattaccta
      481 ccagaacgtt actcctcttc ttcaggttgc ggagcatcgt gaagctggtc gcttcacttc
      541 tatcgagcaa gaatatcccc agatactcaa taaggcacag gcaatccttg ccagagaaac
      601 ggcacatgta aaattccggc cgtggcagga tgataagtgg agtcgagttt tgccgcattt
      661 acgttcggat cgattacagt agattccaat taacaatgag aaacacatag ccaccccgtg
      721 gtattgaaac catataatgt tggatttgaa aacagatctt tttccatgta ttaataacta
      781 catccccgcg agtgattcaa aaaggagggc ccaattttgt ccgagttttt gtattccccc
      841 gcatgccgct gcggagcact acatctgagt gtctgactag gggataaaat tagactggat
      901 agtgagaaga aagtggcgtg ctaggctgtg ccgagtgcta ccagtacacc ctgggggtgt
      961 gcagctttcg ccgagactgt agtgggtatc ggttaatgca cgaaaaaccg agaggtcaga
     1021 caaccaattt gccgtaggat tgtttccggt gcgataccgg tgtactaact gaaagcaatg
     1081 cgaaaaagca taaactcggt ccttcagtcg ccctacacac tatttactaa gaagggctaa
     1141 agcatggata caattatcac atggatggga gatcgtctgt tggggtggat aacaaacaaa
     1201 tccgatctgc ggcagagggc aatcactgga ttaactcctg cgatttgttc aacaatttta
     1261 tatactgaaa aattaaaacg tggtgagcct aataacccaa acgaagaaga aaaactttat
     1321 aggctttggt atgaagcgtc ttcccaagtc gtagactttg atcgagagct ggctaaaaga
     1381 tgtttagata aatcagaata ttggcttcat tctgaattat atagccctga gaaagttgga
     1441 gagctaaaca tttctttggt tggtatgaag gcaacgcttg aaggaataaa gcacaattaa
     1501 aa
//
LOCUS       DAZGXZ010000331         1495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000331 DAZGXZ010000000
VERSION     DAZGXZ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.333"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            135..1486
                     /locus_tag="ACHKJF_005525"
                     /pseudo
     CDS             135..1486
                     /locus_tag="ACHKJF_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
BASE COUNT          431 a          238 c          333 g          493 t
ORIGIN      
        1 ggcaataata attacattca gcaactatca tcatcggtat tgtttgtggg cggaatttca
       61 tattgacaac agtacagttc ttatatctat taataataga aagggatcta aaacctacag
      121 attggtgtag ctttatggaa aaagactatt tgagaattag tagtactgta ttagtgagct
      181 tattgtttgg gcttgctttg gttttggtga atagttggtt taatcagcca ggcgttgagg
      241 aagttgttcc acgatcaacc tatctgatgg taatgattgc tttatttttt atcgatactg
      301 ttgcatttat ttttatgcag ttgtatttca tttatgaccg taggcaattt tcaaactgta
      361 tacttagttt ggcttttctg agttgtttga tttactttgt taaaaccgtc attatcattc
      421 agcaaattat tgaggggcgt ttgacaagca gtgttgtcca aaatgacatt gcaatctatt
      481 atttgtttcg tcagatgagt ttgtgcatat taatatttct ggcattggtg aataaagtta
      541 gtgaaaacac aaaacagcgc aatttatttt ccaaaaaaat gactttgtgc ataagtttgt
      601 ttttgggggg ccgatagttg ctcacatact ttctagtcat tatgagagct acaatttaca
      661 cattgccgag ttaaccaatg aaaatggtca ggtggtttgg aaagcctcat atgttaccat
      721 aatgattttc atgtggttaa ccttgctttc ggttaatcta tactttaatg gtttacgcta
      781 tgatatctgg aatggagtga cggtgattgc tttttgtgcc gtactgtaca atatctctct
      841 gttatttatg agtaggtata gcgtgtccac ttggtatatt agtcgcacta ttgaagtagt
      901 cagtaagtta accgttatgg taatatttat gtgccatata ttcagtgcgc tacgagtaac
      961 aaagaacatt gcacatcgcg atcccttaac caatatattt aacagaaatt atttttttaa
     1021 tgaactgaca gttcaatcag catcagccaa aaaaacgcct tattgcgtca tgattatgga
     1081 tatcgaccac ttcaaaaaag tcaacgacac ctgggggcat ccggttggcg atcaggtgat
     1141 aaaaacagtg gtgaatatca ttgggaaaag catacgacca gatgaccttt tagcgcgcgt
     1201 cggcggcgaa gagtttggcg tcttgctgac ggacatcgat actgaacgtg cgaaagcttt
     1261 agcggaaagg attcgggaaa atgttgagcg tttaactggc gataatcctg aatacgctat
     1321 accacaaaaa gtgacgatta gtattggcgc tgttgtgact caggaaaatg cgttaaaccc
     1381 gaatgagatc tatcgactgg ctgataacgc actttacgag gcgaaagaga ccgggcgtaa
     1441 taaggtggtt gtgagggatg tggtgaattt ttgtgagtca ccataaagcg gcatt
//
LOCUS       DAZGXZ010000332         1485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000332 DAZGXZ010000000
VERSION     DAZGXZ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.334"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            320..1372
                     /locus_tag="ACHKJF_005526"
     CDS             320..1372
                     /locus_tag="ACHKJF_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP0952676.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT          375 a          310 c          329 g          471 t
ORIGIN      
        1 ttacgatgat acaaagcaat aatacttata tgttcgatga ttttaagcac cgagactatc
       61 cggtctttta ctaaataatg gaaaacaatc catccctgga agataattgt aaaaaaaaca
      121 acagaataag aattaatctt aggataaata tttatttatc atggcctttt aaccggtaaa
      181 aaatggcact cggcacagta aaccccgcta tagttatgct atgcatttgt ctttaatgat
      241 atctcgtcgt tatattaaca ggctgaaatt atcattaatg cacatttcat tattagcagg
      301 gatacctgag gagtttaaag tgaacatata tatcgggtgg cttttcaaat taatcccttt
      361 gattatggga ttaatttgca tcgcgttagg tggctttgtg ctggaaagtt cagggcaaag
      421 cgagtatttc gtcgcgggtc atgtgctgat ttctctggcg gccatatgcc tggcattatt
      481 cactaccgca tttattatca tttcgcagct cacgcgcggc gttaatacgt tttacaatac
      541 attgttcccc attattggct atgcggggtc aattatcacc atgatatggg gttgggcact
      601 gttagcaggc aatgatgtga tggcagacga gtttgtcgcc ggccatgtta ttttcggcgt
      661 tggtatgatt gccgcctgtg tatcgacggt ggcagcgtca tccggtcact ttctgctcat
      721 tcccaaaaat gcagcgggga gcaagagcga tggaacgccg gtacaggctt attcttcatt
      781 aatcggtaat tgcctcattg ccgttcccgt tttactcacc ctgctcggtt tcatttggtc
      841 tattacgctg ttacgtagtg ctgacataac tccgcattat gtcgcgggtc acgtattgct
      901 tgggttaacc gcaatctgtg cctgtctaat tggccttgtt gccacaattg tccatcaaac
      961 acgtaatacg ttttcaacta aagaacactg gctgtggtgt tattgggtta tttttctcgg
     1021 ctcaatcacg gtactgcagg ggatatacgt cttagtcagt tccgatgcaa gcgcccgact
     1081 ggctcccggc attattctta tttgcctcgg aatgatctgt tacagcatat tctcaaaagt
     1141 ctggctactg gcactggtat ggagacgtac ctgttcgtta gccaacagaa taccgatgat
     1201 tcccgtcttc acctgcctgt tttgcctttt cctggcatcg tttcttgcgg aaatggcgca
     1261 gaccgacatg ggatatttta ttccttcgcg ggttttggtc ggtttaggag cggtatgctt
     1321 tacgttgttc tcaatcgttt caatattaga agcaggttct gctaaaaaat aattgcaacg
     1381 taccggataa aaccagcgtt gaccatttgc gtaacgctgg tttttcttag gcatcatgaa
     1441 ataacgcagc attaatgcat agtggttaag gataaaaaaa caaca
//
LOCUS       DAZGXZ010000333         1476 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000333 DAZGXZ010000000
VERSION     DAZGXZ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.335"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHKJF_005527"
     CDS             complement(<1..242)
                     /locus_tag="ACHKJF_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011094339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952677.1"
                     /translation="MMKIFKLSTSQPRSPHLWQVAGLKHSTGMSLLGQIREGLDGRVA
                     QLIIDWAKISQADLCKMSGIPPKAFSRSLRTRFSAT"
BASE COUNT          442 a          325 c          296 g          413 t
ORIGIN      
        1 tgtgtggcac tgaatcgagt tctcaggctg cggctgaaag cttttggcgg aataccggac
       61 attttgcata agtctgcttg cgatatcttc gcccaatcaa tgattagttg agcaaccctg
      121 ccatccaggc cttctctaat ctgcccaaga agagacatac cagtcgagtg ttttaaccca
      181 gctacttgcc ataggtgtgg cgacctgggc tgtgatgttg acagtttaaa tattttcatc
      241 attacccgcc ttccgataat tcaggctttt caaaataaat attatattaa aagatcagtc
      301 atatatacga catgctgata tcactggcac tgattctaat atccagtatt taaacagttt
      361 ttcttaagac gaaggtactg tttacccgga acctgcagaa caattaatga aaactactgg
      421 tagaattaaa atgtaagggc aatttttatt atccaactaa tagttttttt gactaaaagg
      481 ttgattggcg gccttatccc cttctgaaag cactatcaaa gggagcaata cgatggttta
      541 ttgttgctcc tgatatgtct gacgggatcc tatggcacag gaaagagatc catgtcaaaa
      601 aacatcgaat gaactgacca catcaagcgg aggatagcat tataaccata tgattacatt
      661 caccttctac tcctccgcac accagaaatt atgccacatt tccacatctt aatttcggcc
      721 gacttaccac cattggtgtg tgattgctac gtagtatcca gtatacccca cagacttagc
      781 atgcgttgca atttaaactg gttccatgaa tctccagaca gttggtatca cttacctgag
      841 tgatagcctt aacactcgtt tttaaaccag cagctagaac ccgatatgat tgtgtcgagg
      901 gtcgcatgtc agcatgatgt gctgccagtc agctgttcct gcggcgttaa ctgaatcagg
      961 aagacagcct cactgccgtt gccgctggtg aactcgttgc gtccgcgtag gggcagtcat
     1021 cgacgatgaa gaaaatcgga gagctaaagc tcctgccggg caagaaaaac atgggaaact
     1081 ggttactgaa aaggtcgttg aatatggctg gcaaaaaaaa tagattatgc acatgccctt
     1141 attaccccgg aacggcgaat tagacttatc aggctttgtc acgagatctc acatgcacaa
     1201 ctgcatacca tggcccatcc ttcgaagaac tagtcggaac gtctgttaaa ccttcggcaa
     1261 ttcagtttta aaattgatag cagttaatcc cactaaatgg tttacccgtt taattatgtc
     1321 agcaaaaaca aaaagttact tttcatgggt atggataaag tcttaaatgc gatgttttac
     1381 attaaaaagc ggacacctcc catcgataaa ttgtctgcaa tctccacaaa ccctgctttt
     1441 caacttaaga aaatgaacat taaaagaaaa atttgg
//
LOCUS       DAZGXZ010000334         1465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000334 DAZGXZ010000000
VERSION     DAZGXZ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.336"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1465
                     /locus_tag="ACHKJF_005528"
     CDS             <1..>1465
                     /locus_tag="ACHKJF_005528"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952678.1"
                     /translation="ENNTQVMIKYKTERGEEKNIKVVVDDTGKYEINLNGWDIKDADG
                     AEVKIVDSAGNVKSGNLYNIARLYVDMNTNKAITLDSAYNIIGSQKEGTDPAKALIMS
                     DDNDWVYIGGGISPHIGDQNPGSDNNIDMAGGDDILSSVGAVLDGANINMGDGDDKLY
                     TQDGFASSGTRNIIMGDGNDVISVDNSFGGKNTISLGEGNNLFIVRNYVNSIAENDIT
                     AGSGDDRIEIGTNLDGKNKIDLGDGDNTIQVGGYITNSHTITGNSGDDIIYVATNIDG
                     SGSFNLGEGNNNFIVGGYIQGKNTIEMGSGDDTVSVSTRIADNVKIQLNAGDDSVYAG
                     GLLNKAIVDLGDGDDVVTLSGISDNGKRNNMEELVSTNAMLTGGEGNDTLKINGSFKL
                     LNMKNISGFETIDLGESSENHLDVGIKSDMLDISSSSGVKIFTIMGGAGNTVDLGKAN
                     ITSHNSVEQGNYADSWYKGDTVDGYTTYTPVGDKSVEL"
BASE COUNT          483 a          191 c          362 g          429 t
ORIGIN      
        1 gtgaaaataa tacgcaagtg atgattaaat ataaaacaga acgtggggag gaaaaaaata
       61 taaaagtagt tgttgatgac acaggaaagt atgaaataaa tctaaatggc tgggacatca
      121 aggatgcaga tggcgctgaa gtaaaaattg ttgattctgc tggtaacgtt aaatcaggaa
      181 acttatataa tatcgcgcgt ttgtatgtgg atatgaatac aaacaaagct ataacgctgg
      241 attcggctta taatataatt ggctctcaga aagaaggaac cgatcctgct aaagctctta
      301 tcatgtcaga tgataatgac tgggtgtata ttggtggagg gatatctccc catatagggg
      361 atcagaatcc aggctctgac aacaacattg atatggctgg cggtgatgat attctgtcat
      421 ctgttggtgc agttttggat ggagccaata ttaatatggg tgatggagat gataaactat
      481 atacgcagga tggtttcgct tcttcaggta cccgaaatat tattatggga gacgggaatg
      541 acgttatttc tgttgataat tcatttggtg gaaaaaatac catttcttta ggagagggaa
      601 acaacttatt tattgttcgt aactatgtaa atagtattgc tgaaaatgat attacggctg
      661 gtagtggtga tgacagaata gaaattggta caaatctgga tggtaaaaac aaaattgatc
      721 ttggggatgg tgacaacact attcaggttg gaggatacat aaccaactca cataccatta
      781 ctgggaatag tggtgatgat attatctatg tcgctacaaa tatagatggt tctggctcat
      841 ttaatttagg tgaaggtaat aacaacttta tagtcggagg atatatccag gggaaaaaca
      901 caattgagat gggttccggt gatgatacgg tttctgtttc cactcgtatt gcagataatg
      961 ttaaaatcca acttaatgcc ggggatgaca gtgtgtatgc aggtggattg ttaaacaagg
     1021 caattgttga cctcggggat ggggatgatg tcgtcacatt gagtggaata tcagataatg
     1081 gtaagcgtaa taatatggag gagttagtat ccactaatgc aatgcttaca ggaggggaag
     1141 gaaacgatac tctgaagatt aatggttctt tcaaattgtt aaatatgaaa aatatatctg
     1201 ggtttgaaac tattgatctt ggggaaagtt cagagaatca tctggatgtc ggtataaaat
     1261 cggatatgct ggatatttca tcgtcatctg gggtgaaaat ttttactatc atgggtgggg
     1321 cgggaaatac agttgattta ggtaaggcaa atataacgtc tcacaattca gtagaacaag
     1381 gaaattatgc tgattcatgg tataaaggag atactgtgga cggatacaca acgtatactc
     1441 ctgtaggtga taaatctgtt gaact
//
LOCUS       DAZGXZ010000335         1452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000335 DAZGXZ010000000
VERSION     DAZGXZ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.337"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..481
                     /locus_tag="ACHKJF_005529"
     CDS             <1..481
                     /locus_tag="ACHKJF_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0952679.1"
                     /translation="RDYSTHSWYAGAEAGYRCHVTEDTWIEPQAELVYGAVSGKQFAW
                     KDQGMHLSMKDRDYNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRD
                     ASGEKRIKGEKDSRMLMSVGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            973..1338
                     /locus_tag="ACHKJF_005530"
     CDS             973..1338
                     /locus_tag="ACHKJF_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952680.1"
                     /translation="MNDGRQTPPSPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAPGILEKYKRTKS"
BASE COUNT          378 a          386 c          366 g          322 t
ORIGIN      
        1 ccgtgattac agtacgcatt catggtatgc cggtgctgaa gcaggctacc gctgtcatgt
       61 cactgaggat acctggattg agccacaggc agaactggtt tacggtgctg tatccggtaa
      121 acagtttgca tggaaggacc aggggatgca tctgtccatg aaggacaggg actacaatcc
      181 gctgattggt cgtaccggtg tggatgtggg taaatccttc tcaggtaagg actggaaagt
      241 gacagcccgt gccggtctgg gctaccagtt cgacctgctg gctaacggcg aaaccgtatt
      301 gcgggacgca tcaggtgaaa aacgtatcaa aggtgaaaaa gacagccgta tgctgatgtc
      361 cgttggcctg aatgcagaaa tcagggacaa cgtccgcttt ggactggagt ttgagaaatc
      421 cgcctttggt aagtacaacg ttgataatgc tgtcaacgct aacttccgtt actcgttctg
      481 atactccgta ttcaaaaggg cctgcgggcc ctttatgctc aggacctgcc gcccgggtat
      541 acatttccgg ggtatcagtc gatttgttta cttaaccgcc atgaccaaag ccggcagcaa
      601 tgtcggcttt ttacgtccat atattccact catccggaac tgtgacacag aacctgaata
      661 caggattgat caccggtgcc cacaaatggc acattttcag taagattctg aatgttacat
      721 tcccccgtca tgtgtggcac aactgtcacc gggaaagaaa cggaaagcac atgaaaaaca
      781 tcaaaactca caccggactc cttatccaca aagaacaagc gcggcgcgtt cgtcttcatg
      841 aaactccggc cgcctggtgc cacacgcacc gggaatgcta cagcaaaacg accggtcgcc
      901 gctgcggcag ccctgacagt ctcagcaggc tgattctgag cagcattcag cccatagaat
      961 gagatgaccc gtatgaatga tggcagacag acgcctccgt cccctgaggc atggaaaatc
     1021 cggtttctgg cgaacggtac acccggcaca tggttttaca ccgatgacgc agccgtcaca
     1081 aaagagtggc tgcgggcaca ttgtgacgat gtggaagtga tccccctgtg ccgtatgcat
     1141 gacggtcccg ttttccggga acaccatatt aatgaactca gcgccgcact ggccagaatg
     1201 ctccatatgc accacatact gctggaacga acccggtaca gagaaaatat ccatgatgca
     1261 gaatgtattc gtgaaattca cgaagctccc ctgcaggctc ccggcattct ggaaaaatac
     1321 aaacgcacaa aatcataagg cagttctatg tggatactga ttttctggct ctcatccccc
     1381 tttaatgtct cctccggcga tgcccggcaa cccacactgg ccgttcacag tcaggagttc
     1441 cgtactgaaa aa
//
LOCUS       DAZGXZ010000336         1423 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000336 DAZGXZ010000000
VERSION     DAZGXZ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.338"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            8..853
                     /locus_tag="ACHKJF_005531"
     CDS             8..853
                     /locus_tag="ACHKJF_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0952681.1"
                     /translation="MSDITVSRPEVVNGHTDVICSTSILHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTALDWAMKQNFHCGCWLMEKPETAMKTITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISKANILSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHFITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIKYFKKGSVHITFRKPELVDRLNDIIARHYPE
                     MLPSQ"
     gene            1087..1215
                     /gene="rhuM"
                     /locus_tag="ACHKJF_005532"
                     /pseudo
     CDS             1087..1215
                     /gene="rhuM"
                     /locus_tag="ACHKJF_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          408 a          332 c          294 g          389 t
ORIGIN      
        1 atcgattatg tctgatatca cagtctcccg tccggaagtt gtcaacgggc atacggacgt
       61 tatctgctca accagtatcc tccacattct ggctgtacga aagagtacac tgctgcaaat
      121 cgacacactt atccggcaac tggctgaaat ctcagcaatg acagaaagca ttggcggtaa
      181 aaccgcactg gactgggcca tgaaacagaa ttttcactgc ggttgctggc tgatggagaa
      241 acctgaaacc gcaatgaaaa ccatcacacg caatctcgat cgcgaaatct ggcgtgacct
      301 gatgcaacgt tcagggatgc tttccttaat ggatgcacag gcccgtgata catggtaccg
      361 gtcactggaa tacgataatt ttccggaaat cagtaaagcg aacattctga gcacatttga
      421 acaactgcac cagaataagg atgaggtgtt tgagcggggg gtgatcaacg tcttcagggg
      481 actgagctgg aattacaaaa ccaactcacc ctgtaaattt ggcagtaaaa ttatcgtcaa
      541 caacctagtg aggtgggacc ggtgggggtt tcattttatc accgggcaac aggcagatcg
      601 acttgccgat ctggaaagaa tgctacacct gttcagcggc aaaccgatcc ccgacaaccg
      661 ggaaaacatc acccttcgtc tggatgatca catccagtct gttcagggta aagaggacta
      721 tgaagacgag atgttcagta ttaaatactt taagaaaggc tcagtacaca tcacgttcag
      781 gaaaccagaa ctggttgaca ggctaaatga tattatagcc agacactatc cggaaatgtt
      841 accttcacaa tagagtacta agaattgatt caattatgta ttttagcttc attccttcat
      901 gcaaaatcat ttgggggttc ttatgggggt tactcattta tccaacacat acaaattatt
      961 taagatcaat gcattaatac ttatattcga ctcctgtgat ctccgccaaa atgcctctcc
     1021 tgacgttctc caaccccttg taatactgtc aatccagata ttttctactg ctcaatattc
     1081 aatcggcttc aggaatacct ggtctaaggt tttgtgatgg acgatgagcg gctgaagaat
     1141 ccgcctgtcg gttcttctgt cctcgaacgc atccgcgata ttcacgccag tatgcgccgt
     1201 gtttatttgc aggtgatttg ccccccctgc tgactctcag ccaactgtat cgccattaag
     1261 gtgaagatgt tgttttactc gccccagaac accctttttt atacgactat tctatttctt
     1321 taaccgctgg cgaaaaaaac tgactataaa cttcaccaat aatcagactg ccatcaagat
     1381 taatttgttc atttaatata tcaaatcaac ttgttttatt ttt
//
LOCUS       DAZGXZ010000337         1389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000337 DAZGXZ010000000
VERSION     DAZGXZ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.339"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(176..1327)
                     /locus_tag="ACHKJF_005533"
     CDS             complement(176..1327)
                     /locus_tag="ACHKJF_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFP0952682.1"
                     /translation="MDKSLSRSVISVVIGGILFGVSAGVLAQDASENKLIASGSIPEN
                     TDLSASARADGEIALAVGTGASADKEIATSVGSWSAARGQQSVALGGGTYAYADASTA
                     LGSIAFVDNTATYGTAAGNRAKVDKDATEGTALGAKATVTNKNSVALGANSRTTRDNE
                     VYIGYEAEPGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEA
                     KKSREYTDSRTTVGVNSDGTLTRAEGASKTLAVNDGLVALSGRTDRIDATVGSVDRRV
                     TKNTQAIQSTSRQLQEHNTRLNSHQRQINENHEEMKRAAAQSAALAGLFQPYSVGKFN
                     ATAAVGGYSDKQAVAVGVGYRFNEQTAAKAGVAASDGDVSWNAGVNFEF"
BASE COUNT          341 a          395 c          286 g          367 t
ORIGIN      
        1 tccctcgcca gtattatcct gtacccctca agcatctgct gcaggccata ctccagataa
       61 cggttgcggg tcgccagaat cactttcatc gctgccacct gtttcatgct cacatgagaa
      121 aaaacggcag acaaaactcc acgcctgccg tcatgcttca tgtcactgtc agcaattaaa
      181 actcaaagtt cacaccagcg ttccaggata catcgccatc gctcgctgcc acacctgctt
      241 tcgctgctgt ctgctcattg aaacggtaac caacacccac ggcaacagcc tgtttgtcac
      301 tgtaaccacc aacggcagca gtggcgttga atttacccac actgtacggc tggaaaagac
      361 ctgccagcgc tgcgctctgg gctgccgcac gtttcatctc ttcgtggttt tcattaatct
      421 gacgctgatg actgttcaga cgtgtgttgt gctcctgcaa ctgacgggaa gtggactgaa
      481 tagcctgtgt atttttagta acccggcggt caacactacc aacagtagca tcaatacggt
      541 ctgttctgcc ggacaaagct acaagcccat cattaacagc cagggttttg ctggctcctt
      601 ctgcacgggt cagggttccg tcggagttaa caccaactgt cgttctggag tctgtatatt
      661 cacgagactt tttcgcttca accacaaggc gggtattcgt atcctgagcc agttgctcaa
      721 cactgtcttt tacacggtca acctgacgaa cggttgcagc atctgaggga cgagtaccgt
      781 cactcagacc accaagcacc cgggttttat aagccttacc cggctctgct tcataaccga
      841 tataaacctc attgtcacgg gttgtgcgtg aattagctcc taatgcaaca ctgtttttat
      901 ttgttactgt tgcctttgcc cctaacgcag taccttctgt tgcatcttta tcaactttgg
      961 ctcggttacc tgctgccgtg ccatatgtag ccgtgttatc aacaaatgca atgcttccca
     1021 gggctgtaga tgcatctgca taagcatatg ttcccccacc taaagccaca ctttgctgtc
     1081 ctcgtgctgc agaccatgat cccactgatg ttgcaatttc tttatccgcg ctggcccctg
     1141 taccaacagc tagcgcaatc tctccatccg cgcgagctga agcagaaaga tctgtatttt
     1201 ctgggatact ccctgaggcg ataagcttat tctccgatgc atcctgagcc agaacgccag
     1261 cgcttacacc aaataaaata ccgccaataa caacggaaat aacacttcgt gataaagact
     1321 tatccatgta tgattcctca tgaatttatt tgtatacaaa caaaccgaaa tacatcggat
     1381 gaataaaga
//
LOCUS       DAZGXZ010000338         1386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000338 DAZGXZ010000000
VERSION     DAZGXZ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.340"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(1..425)
                     /locus_tag="ACHKJF_005534"
                     /pseudo
     CDS             complement(1..425)
                     /locus_tag="ACHKJF_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(447..986)
                     /gene="yhaB"
                     /locus_tag="ACHKJF_005535"
     CDS             complement(447..986)
                     /gene="yhaB"
                     /locus_tag="ACHKJF_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP0952683.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTPPTHFNKTCIT
                     SDYWQILFFSPDHFNNDFY"
     gene            complement(1242..>1386)
                     /gene="tdcR"
                     /locus_tag="ACHKJF_005536"
     CDS             complement(1242..>1386)
                     /gene="tdcR"
                     /locus_tag="ACHKJF_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFP0952684.1"
                     /translation="RYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECN
                     RFP"
BASE COUNT          423 a          238 c          258 g          467 t
ORIGIN      
        1 attacgttca acctcctgat ttcattgtaa atatctttta atgggcaatt aacattgata
       61 ttgcctgtag atatttccta gttttttaac ggttcattaa tggtaagttc gcaggtattg
      121 tccgatcgtt gaataatttg ggcgttataa cctaaaaaat ccagacgaag agacgagtca
      181 ttataaagaa ttacattttg taagtacgct aattcttttt gaaaatcacc agtgataatg
      241 cattttccac ctggttgtaa cgcgttaagt aaagtgacca tttcaataga ggtctgtttt
      301 agtttttcta tacctggtgc tagtgcttcg aggttatttc ctatggggct ttcaggaagg
      361 tcattcattt ttcgacggac taaatcacta ctaatatcat taccaattga tgaaacaggg
      421 gacattattt tatcctttat aaataatcaa taaaaatcat tattaaaatg atcgggtgaa
      481 aaaaagagaa tttgccaata atctgaagta atgcaggttt tattaaagtg cgtagggggt
      541 gttaagatgg cttctttttg ggtgaacttt gcgcaatact tagaaatcgt ctccttaata
      601 cattccagct cttttttatt gacggctgag caagtacatt gttcaccaaa aggtgcaaaa
      661 gtgcctacat ctaatgtttg tatgatataa tcgtcatgtt ctcctgtaga aatatctata
      721 atgaatggac tcatattttc cggttggatt cggatcatga cagaaggaac atcgccttga
      781 tagaaattca atgcagtaat tgcagataat actctccgca gttcattact tatactgtaa
      841 attttattaa cgtcattggt ggagacaatt tctgcaaact ttctttttac cgtccaataa
      901 tcaatatttc tattgtgatt gttaaccact gcgtgcattg gcgggattga agggtgaaaa
      961 atatgtgcaa ttgggaagcc tttcattgat gcctgccttg tcatcatgac aaacaataat
     1021 agatgcttat tttcctcgca gggttctttc gcactatcaa aaaacgctca tttacccggc
     1081 gttacagtaa acgaagcaac attctgtggc ctacgacgat caaaagctct gtgaaaattt
     1141 cccttgttgt ctttgtctga ccttatcgcg gcgatcagca attattcgtt ttgtgatcct
     1201 caacacaaaa tgctattcgt cgcctatgag gggagaagaa tttatggaaa acgattgcat
     1261 tcacaaaatg acgtcctgtc agaagagaaa aagttgacgt taatcaattg ttcgagatga
     1321 tcaatacctg tattcgaaat tttaaaatat tgataaaata aatgtttgtt ctccaacaaa
     1381 tagcgt
//
LOCUS       DAZGXZ010000339         1386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000339 DAZGXZ010000000
VERSION     DAZGXZ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.341"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1386
                     /locus_tag="ACHKJF_005537"
     CDS             <1..>1386
                     /locus_tag="ACHKJF_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP0952685.1"
                     /translation="FSALKDRHTAVEVNYTDPQNGWQTSTELVEDPDAILRYGRNLLK
                     MDAFGCTSRGQAHRAGLWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTL
                     TGGRILSIDAASRTLTLDREVTLPETGTSTVNLINGSGKPVRVDITAQPAPDRIQVSI
                     LPDGVATYGVWGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFE
                     PMSGSLNSVIPPAVQHLTVEVSASDGQYLALAKWDTPRVVKGVRFSLRLTSGSGENSR
                     LVTSAITADTEHRFSGLPLGEYTLTVRAINSYGQQGEPATTTFRINAPAAPDSIELTS
                     GYYQITAVPVLAVYDPTVQSEFWFSEKRITDMAQVETFARYLGTGSQWSVSGPHIKPG
                     KDFWFYVRSVNLVGKSAFVEASGRASNDAEGYLDFFRGEIGKTHLAQGMWELIDNSQL
                     DDEMAEMKTTIT"
BASE COUNT          304 a          388 c          444 g          250 t
ORIGIN      
        1 cttcagtgcc ctgaaggacc ggcacacggc ggtggaggtg aattacaccg acccgcagaa
       61 cggctggcag acttccactg aactggtgga agacccggac gccatcctgc gctacgggcg
      121 caatctgctg aagatggatg cgtttggctg taccagccgc ggtcaggccc accgtgccgg
      181 actgtgggtg ataaagacag aactgctgga aacgcagacg gtggatttca cgctcgggtc
      241 acaggggctg cggcacacgc ccggtgacat cattgaaatc tgtgataacg actatgccgg
      301 gaccctgacc ggcggacgta tcctgtccat cgatgccgcc agccgtaccc tgacgctgga
      361 ccgtgaggtg acactgccgg aaacagggac atcgacggtg aacctgatta acggcagcgg
      421 taagccggtg cgcgtggaca tcactgcaca acccgcccct gaccggatac aggtcagcat
      481 tctgcctgat ggcgtggcga catacggtgt gtggggactc tccctgccgt cactgcgtcg
      541 tcgcctgttc cgctgtgttt ccatccggga aaacacggac ggcacctttg ccatcacggc
      601 agtgcagcac gtaccggaaa aagaagccat cgtggataac ggggcccgct ttgagccgat
      661 gtccggctca ctgaacagcg tcatcccgcc ggcagtgcag cacctcacgg tggaggtgag
      721 tgcctcagac ggccagtatc tggcgctggc gaaatgggac acgccgcggg tggtgaaggg
      781 cgtgcgcttc agtctgcgcc tgaccagtgg cagtggtgaa aacagccgcc tggtgaccag
      841 cgccatcact gccgacacgg agcaccgttt cagtggcctg cctctggggg aatacaccct
      901 gacggtcagg gcgataaaca gctacggcca gcagggcgaa cctgccacca ccacgttccg
      961 gattaacgcc ccggcagcac cggacagcat tgagctgacg tcggggtatt atcagataac
     1021 agcagtaccg gtgctggcgg tgtatgaccc gacggtacag tctgaattct ggttctcaga
     1081 aaaacgcatc acggacatgg cacaggtgga aacctttgcc cgttatctgg ggacaggcag
     1141 ccagtggagt gtctccggcc cgcacattaa gccggggaag gatttctggt tttatgtgcg
     1201 cagcgtcaac ctggtgggga aatctgcgtt tgtggaagcc agtggccggg cgagcaatga
     1261 tgcggaaggg tatctggact ttttcagagg agaaatcggg aagacacatc tggcacaggg
     1321 gatgtgggag ctgattgata acagccagct tgacgatgag atggcggaga tgaagaccac
     1381 catcac
//
LOCUS       DAZGXZ010000340         1378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000340 DAZGXZ010000000
VERSION     DAZGXZ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.342"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            122..1141
                     /locus_tag="ACHKJF_005538"
     CDS             122..1141
                     /locus_tag="ACHKJF_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0952686.1"
                     /translation="MKYIPVGVDIAKHLMQVHFVDEHTGEIIDKQVKREKFLEYFSNR
                     EPCLIGMEACGGSHHWARELQKLGHKVRLLKGRFVKAFVMGNKNDVMDARAIWLAVQQ
                     PVKSVAVKSEEQQATLGLHRMRQQLVKFRTAQINALHSLLLEFGETTHKGRAALAKEV
                     PEALERLKERLPLYLINMLDEQYQRITELDEHLDHIEKQLQAIARQNETCQRLLNIPG
                     VGPLIATAAVATMGDASAFKSGREFAAFIGLVPRQTGSGGKIRLLGISKRGDKYLRTL
                     FIHGARAAALLTKEPGPWITELKKRRPASVAIVAMANKLARTVWAIAAHGRKYDKNHV
                     SIRPD"
BASE COUNT          384 a          328 c          385 g          281 t
ORIGIN      
        1 ccaacattat gaaatgagcc gctcggggat taagtagatg agtgtggatc cccgcagatt
       61 tccggcatca tcgtgcccaa cgggatgcgt ttcgctccca acagactgga ggatccacac
      121 catgaaatat atccctgtcg gcgttgatat tgcaaaacat cttatgcagg tgcatttcgt
      181 tgatgaacat accggagaga ttatcgataa gcaggttaag cgtgagaaat tccttgaata
      241 tttcagcaac cgtgaaccct gccttatcgg catggaggcc tgtgggggtt ctcatcactg
      301 ggcgcgtgag cttcagaaac tgggacataa ggtcagatta ctcaaaggcc ggttcgtcaa
      361 agcattcgtg atgggaaata aaaacgatgt gatggacgcc cgagccatct ggctggcggt
      421 tcagcagccg gtaaaatccg tcgccgttaa aagcgaggag caacaggcca cgctggggtt
      481 acaccgaatg cgccagcaac tggtgaagtt caggacagca cagataaacg cgcttcacag
      541 tctcttgctg gaatttggtg aaacgacgca caaggggcgt gctgctttgg cgaaggaggt
      601 gccggaagcc ctggagcgtc tgaaggagcg gctaccgctc tacctgataa acatgctgga
      661 tgaacaatat cagcgaatta ctgaacttga tgaacatctt gaccacattg agaagcagtt
      721 gcaggcgata gccagacaga atgaaacctg tcagcggtta ctgaacatcc caggcgtggg
      781 gccgttaatc gccaccgctg ccgttgccac gatgggagac gcctccgcct ttaagtcggg
      841 acgggaattc gccgcgttta tcggtctggt gcccagacag acaggctcag gaggaaaaat
      901 ccgcctgctg ggaataagca aacgtggtga taaatatctg cgaaccctgt ttattcatgg
      961 tgcaagggcg gcagcattac tgaccaaaga acctggtcca tggataaccg aactgaaaaa
     1021 gcgtcgtccg gccagcgtgg caatcgtcgc aatggcaaac aagctggcgc gaacggtgtg
     1081 ggcaatagcc gctcacggac gtaaatacga caaaaatcat gtcagtatca ggcctgatta
     1141 atcgctgata ccgataaaca taaactcttt acaaaagggt gaatgcagaa aggttgctct
     1201 ggcagccagc gtgatgacaa agacaggtaa gaccgtgact cactaaacct ggataatcga
     1261 ttgggcctaa agcccgcaga ggaaatgagg cgtgagtcgg cgaattacat aggggctcgc
     1321 agcttccggg ctgcaataaa gccggatata aagctgcaac ctaccgtcga tgtcagaa
//
LOCUS       DAZGXZ010000341         1369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000341 DAZGXZ010000000
VERSION     DAZGXZ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.343"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1134
                     /gene="ompC"
                     /locus_tag="ACHKJF_005539"
     CDS             <1..1134
                     /gene="ompC"
                     /locus_tag="ACHKJF_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0952687.1"
                     /translation="LSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRVTALHYFSDDKG
                     NDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAFAGLRFAD
                     VGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNTDFFGLVD
                     GLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGFVAAYTKSDRTDKQVQGLNGT
                     ADVLDPESGKKVGEKTVDSGSVAKGKHAEFWGTGLKYDANNLYLAAVYSETQNMTTFG
                     DQGVADKAQNIEAVVQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQDLVKYIDVGAT
                     YYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
BASE COUNT          346 a          253 c          359 g          411 t
ORIGIN      
        1 ctttctgctg tagcagtggc agtgatgttt gctgcggggg cgcaggcagc agaagtttat
       61 aataaagatg gtaataaact ggatctttac gggcgtgtaa ccgctctgca ttacttctcg
      121 gatgataaag gtaatgacgg agaccagact tatgctcgtc tcggctttaa aggcgaaacg
      181 cagattaatg atcaactgac cggatttggt cagtgggaat accagttctc tggtaacaaa
      241 acggaatctg aaggttccgc gggaaataaa acccgtctgg catttgcagg tctgagattt
      301 gcagatgttg gtaccattga ttacggacgt aactatggta ttgcttatga tgtcggatca
      361 tatactgacg tactgcctga gtttggtggt gatggctgga cgcagaccga taactttatg
      421 acggcccgaa cttccggtgt cctgacttac cgtaatacag acttcttcgg gctggttgat
      481 ggtctgaatt ttgcggcgca gtatcagggc aaaaatgagc gtgatgacct tcagaaggct
      541 aatggcgacg ggtatggttt ctcggccagc tatgagtttg atggttttgg ttttgtcgct
      601 gcgtatacca agtcagatcg taccgataag caggttcagg ggttgaatgg tactgcagat
      661 gttcttgatc ctgaatcagg taaaaaggtt ggagaaaaaa cagttgactc aggtagtgtg
      721 gcaaaaggta aacatgctga attctgggga actggtctta aatatgatgc caataacctg
      781 tatctggcag cagtgtattc tgaaacccag aatatgacga cctttggtga tcaaggtgtt
      841 gcggataaag ctcagaatat cgaagccgtt gttcagtatc agtttgattt tggtctgcgc
      901 ccgtcccttg cctaccttca gtcccgcgga caggatgtta tggttggtgg tgtgaaccat
      961 ggcgatcagg atctggttaa atatattgat gtcggtgcga cttattactt taacaagaat
     1021 atgtccacct atgttgatta taaaattaac ctgattgatg aaagtgaatt tacccggaaa
     1081 gccggtgttg cgacagataa tatcgttgct gtgggtatga cttatcagtt ctgattattg
     1141 ctgataagtt aataaatacg ggccgtctgc ccttacaggc ggcctgtatc aatgaaaaca
     1201 cagttttcat tggtcactgc gatcagcaat tgccatctgg catgtgctga tttaactttc
     1261 tgttattacc tttattggtt ttattttaaa ttggactttt attgttcggg gcgcgtctgc
     1321 gccccttttt tatgggcgga tatatgcagg atattcacga agaaagcct
//
LOCUS       DAZGXZ010000342         1365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000342 DAZGXZ010000000
VERSION     DAZGXZ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.344"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(354..1179)
                     /locus_tag="ACHKJF_005540"
                     /pseudo
     CDS             complement(354..1179)
                     /locus_tag="ACHKJF_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          355 a          322 c          295 g          393 t
ORIGIN      
        1 caacaaaggg aaaggtctgt agataaagag tgggatggac ttcttacttc atatcacata
       61 attgatattt tttatcctgc aaactccaac ggcattatgt atgacttagc ctcaggcaat
      121 tatcacaggc aaaaaaacaa tatattgtaa gcttcataga atttggaact ttatataata
      181 aataatgctg agttttttat caccaatacg ttaacaaaat taatgtattc cagaggtttc
      241 acattagtct ccaatttttc agaagaatct atttacctgg caggaaaagt catctggtgt
      301 ttcgccggag tgaggggtac aggcaatagc ggacataacc atttgttctt tttctaagca
      361 gccatctgat aatattttat cctgaaggct gtcggggaga tattccccag acgagagtga
      421 cgacgctgac gattgtagaa aatctcaatg tattcccgta ttactgagat ggcctcatcc
      481 cggcttttaa aacgatagtg gctcagactt tcatttttca gcgttcccca gaagctttcc
      541 ataggagcat tgtcgtaaca gttaacttta cgcgacattg atattttcag accaaactgc
      601 tcctgtatgg ccctgtaatc gtatgcgcag tactgtgaac cacgatcgga gtggtggatt
      661 agcccggtag gtgagcgctg gttcctgagc gccataaaca gggctttacc cgtcaactat
      721 ttcgtcatgc gctctcccat ggcgtaaccg acaattttgc tcgtataaac atctttgacg
      781 ccagcgatat acagccatcc ttccgggggg caacatacgt tatatctgca accaagacct
      841 gattaggtgc tgtaggcgta aagttctgat tcaggagatt cggcgcaacc ggcagattgt
      901 gacaagagtt cgtgttcgcc ctgaacttgc gtttctgctt acagtgcagc ctcagctcct
      961 tacgaagacg tgccagtctg tcacgaccga cgataattcc gttatctgcc agctccgtct
     1021 ggagcagcta ggttccatat gtttcgcgtg tgcggatatg tgccacctta atctccagtt
     1081 ttagccgctc atcactttgt tttctgtctg agggtcatgc tgtactcagt cgtaataacc
     1141 gatcctgtat acaccaagta cccgacacat cgcttcaata cataatcgca tctgaatcgt
     1201 tcaccgattc gcggcatttt tcaaggtagt tgtcgccatt gttcgactaa ctgatcgaca
     1261 ttacagccta gagcctagct gggggctgtg cacaaaaaag ccccggacat tccggggcta
     1321 cactgcatat taacgaacgc tctgtccggt tatttcaacc ctgcg
//
LOCUS       DAZGXZ010000343         1349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000343 DAZGXZ010000000
VERSION     DAZGXZ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.345"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..351
                     /gene="traE"
                     /locus_tag="ACHKJF_005541"
     CDS             <1..351
                     /gene="traE"
                     /locus_tag="ACHKJF_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HFP0952688.1"
                     /translation="MALAFIALRLNVSPEIVDASHQALLQYIRPGAQNKMKVILAEEA
                     KLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTDIKHYILILKRENGV
                     TWLDNFGETDDEKK"
     gene            338..1066
                     /gene="traK"
                     /locus_tag="ACHKJF_005542"
     CDS             338..1066
                     /gene="traK"
                     /locus_tag="ACHKJF_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFP0952689.1"
                     /translation="MRKNKTVIISACLLFSGSVLAGNGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGETAGAWETSTPYESLLVSISQAVRAGKLPEGWYQI
                     PVTKETLQAPAGMSAKAEAVWTGNHLKMVRFTVENKTLSALNIRESDFWQPGTRAVMF
                     SQPVSQLLAAARMDVYVIRDGEGN"
     gene            1066..>1349
                     /locus_tag="ACHKJF_005543"
     CDS             1066..>1349
                     /locus_tag="ACHKJF_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HFP0952690.1"
                     /translation="MASINTIVKRKQFLWLGIVIVGSVSAIGGALYLSDMDMSGNTDA
                     VAGQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRREMDVL"
BASE COUNT          331 a          320 c          386 g          312 t
ORIGIN      
        1 atggcgctgg cgtttattgc cctgcgcctg aatgtctcac cggaaatcgt cgatgcttct
       61 caccaggcac ttctgcagta tatccgtccg ggcgcgcaga ataagatgaa agtgattctg
      121 gccgaagaag caaagctcat taaaaaagat aatgtgaatt ctgctttttt ccagaccagt
      181 gttcgtgtct ggccacaata tggtcgtgtg gaaatccgtg ggattcggaa aacatggatt
      241 ggtaactccg aacctttcac tgatatcaaa cattacattc ttattctgaa acgggaaaac
      301 ggggtgacct ggctggataa tttcggggaa acagacgatg agaaaaaata agacagtaat
      361 aatatccgcc tgcctgcttt tttccggcag cgtgctggca ggaaacggca cactggcacc
      421 tacagtggtt ccgatggtca atggcggtca ggcaagtatt gccatcagta ataccagccc
      481 gaatctgttt accgtccccg gtgaccggat tatcgccgtg aacagtctgg atggtgcgct
      541 gaccaataat gagcagactg catccggtgg tgtggtggtg gccaccgtca acaaaaagcc
      601 ttttacgttc attctggaaa cagaacgtgg tcttaatctt tccattcagg ccgttccccg
      661 tgaaggagcc gggcgtacca ttcagctggt cagcgatttg cgcggaacag gagaaactgc
      721 cggtgcatgg gaaacatcca cgccttacga atccctgctg gtttccatca gtcaggcggt
      781 ccgtgccgga aaactgccag aaggctggta tcagatcccc gtgacaaagg aaaccctgca
      841 ggcccctgcc ggaatgtccg caaaagctga agccgtatgg acggggaatc acctgaagat
      901 ggtgcgcttt accgtggaaa ataaaacgct gtctgcgctg aatatccggg aaagtgactt
      961 ctggcagccg ggaacacggg cggtgatgtt cagccagcct gtcagccagt tactggccgc
     1021 tgcccgtatg gatgtgtacg tcattcgtga cggggagggc aactgatggc cagtatcaac
     1081 accattgtga aacgcaagca gttcctgtgg ctggggattg tgattgtcgg atcggtatca
     1141 gcaattggtg gtgcgcttta tctgtctgat atggatatgt ccgggaacac tgatgccgtt
     1201 gcagggcagg agcctgtgcc ggatatgacc ggtgttgtgg ataccacctt tgatgacaag
     1261 gtgcgtcagc atgccaccac agagatgcag gtgacggcgg cacagatgca gaagcagtat
     1321 gaggaaatcc gtcgtgagat ggatgttct
//
LOCUS       DAZGXZ010000344         1319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000344 DAZGXZ010000000
VERSION     DAZGXZ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.346"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1032
                     /locus_tag="ACHKJF_005544"
     CDS             <1..1032
                     /locus_tag="ACHKJF_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0952691.1"
                     /translation="QPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFP
                     AELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDH
                     RGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNR
                     YYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTS
                     SIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSE
                     TKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLAC
                     RTWQECWVN"
     gene            1044..>1319
                     /locus_tag="ACHKJF_005545"
     CDS             1044..>1319
                     /locus_tag="ACHKJF_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-e4"
                     /protein_id="HFP0952692.1"
                     /translation="MKKYIFLVLFLLCGCSPVDIEPARQEVNIFRGLYQTGKYSDIYK
                     ITSSDFQTATSEKKFIDIMNEAKEKDLGTYKKSPLKTEKITRGLFLND"
BASE COUNT          374 a          254 c          371 g          320 t
ORIGIN      
        1 cagccgggga gcttcgcgcc gctcatccgc atcgaaacag acaacggcga gcgggagaaa
       61 gcgcagcgcc gcagcctggc ggagaagctg cagcaggaag ggagcgagga cgggcacggc
      121 gtggtatttc cggctgaact ggtgcggctg ctggacaggc tggaggaaga aatccgggca
      181 gaccgcgtga gcagtgaaag ccgggcgtgg cttgcgcagt gcgggctgac ggtggagcaa
      241 ctggccagac aggtggagcc ggaatacaca ccggcgcgaa aagttcattt ttaccactgc
      301 gaccaccggg gcctgccgct ggcgctcatc agcgaagacg gcaatacggc gtggcgcggg
      361 gagtatgatg aatggggcaa ccagcttaat gaagagaacc cgtattacct gcaccagcca
      421 taccgtctgc cggggcagca gcatgatgag gaatcagggc tgtactataa ccggaaccgg
      481 tactatgacc cgctacaggg gaggtatatt acacaagacc ccattgggct ggcgggggga
      541 tggaacctgt ataattaccc actgaatccg ataataagga tggatccttt gggtttgtat
      601 aatttatatc aattattata tgatgtttgg catgatgatt catatggaac atcatcaatt
      661 gatattactg gcagtggaga tctaatatca ttaggtggtc atgcaggact tggcgttgcg
      721 tttgctaaaa agaaaggtga aatgttatct gatatttgta tttatgctac agcatgcgga
      781 catgcaggaa ttggtggtgg gataaatgcg gctatcacat attctgagac caagtcttta
      841 cctacatcgg gagtcagcaa ttcagtaggt gtaacggttg gcggcggagt tggggggcat
      901 tttgcgtata cttatgtagt ggatgttgat aatccagaat catcgacaga atctgttggt
      961 atcggtgcag gtgttgacgc ttcagttatg actctggctt gtagaacgtg gcaagaatgc
     1021 tgggtcaatt aacaggagga aatatgaaaa aatatatatt tttagtttta tttttattgt
     1081 gtggttgttc tccagtggac atagagccag ctagacaaga agttaatatc tttcgtggtt
     1141 tatatcagac agggaaatac agtgatatat ataaaatcac ttcaagtgat tttcagactg
     1201 ccacatctga aaaaaaattt atcgacataa tgaacgaagc aaaagaaaaa gatcttggga
     1261 cttataaaaa atcacccctt aaaactgaaa aaatcactcg cggattattt ttaaacgat
//
LOCUS       DAZGXZ010000345         1292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000345 DAZGXZ010000000
VERSION     DAZGXZ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.347"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..247)
                     /locus_tag="ACHKJF_005546"
     CDS             complement(<1..247)
                     /locus_tag="ACHKJF_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFP0952693.1"
                     /translation="MCAMDRRERALIRQLHFPNDLRANISLLKYPVCIRFEDGSFIGT
                     NHSFERFIKLSYCSCDEWFEHLTLESKLQLSRSEMDSY"
     gene            complement(438..821)
                     /gene="traM"
                     /locus_tag="ACHKJF_005547"
     CDS             complement(438..821)
                     /gene="traM"
                     /locus_tag="ACHKJF_005547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFP0952694.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKKSAFNQIEFNKLLLECVVKTQSSVAKILGIESLSPHISGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDDE"
     gene            1144..>1292
                     /locus_tag="ACHKJF_005548"
     CDS             1144..>1292
                     /locus_tag="ACHKJF_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HFP0952695.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICQA"
BASE COUNT          413 a          248 c          219 g          412 t
ORIGIN      
        1 aatatgaatc catctcagat cttgatagtt gtaacttgga ttctaaagtc aaatgctcaa
       61 accattcatc acatgaacaa tatgataatt taataaatcg ctcgaagctg tgatttgttc
      121 ctataaaaga accgtcttca aaacgtatac atacgggata tttcaataat gaaatgttcg
      181 ctcttaagtc gttagggaaa tgtaattgac gaatgagtgc tctttctcta cgatccattg
      241 cacacatagg aacctcctca aaggattcta tggacagtca atgcagggag cgggtactcc
      301 ctgcattggc gatttaaact aaatttttaa cgtggcatta accacataat taatataccc
      361 tttatgtggt taattgtcat caaattgaac cagatcaaaa ctctgttatt tgaagtaaat
      421 caacttttaa ttctttttta ttcatcatca ttttttggaa aaaatcgttc catctcagat
      481 gacaccttct cccggatatc ttcaaccata ttggcatatt caaactttgg attcccggag
      541 atatggggac ttagagactc aatacccaaa attttcgcta ctgatgattg tgtttttaca
      601 acgcattcaa gaagtaattt attaaactca atctgattaa acgcggactt tttacgctcc
      661 atctgagctt catatacacg aagccctagt tcaagaagca ttgaagcagt tcccgatacg
      721 cttatatctt tatctcttgc gccctcctga cgacgctgtt caactatcgc atttacttta
      781 tcatagacat cattactgat atacaaaatt actctggcca taagataaaa cctttcatta
      841 ttaagcaatg aacttttcac tataaatatg catatagtgt ttacaagtaa gaaagacact
      901 cctagcagcg cctctaggat catcctataa aaaaatgcga tccggcgcta ggggcgtccc
      961 taatatatat caatgttttt catgaaaatt gtcagtactg atcctaataa gagtcgctat
     1021 agggtcgtaa caggatcgcc aacgactctc tatttaataa ttcagaatta ttaaatataa
     1081 atagcattta ttaattacat gatttaaaac gcaaatcagc aaaaacttgt ttttgcgtgg
     1141 ggtgtggtgc ttttggtggt gagaaccacc aacctgttga gcctttttgt ggagtgggtt
     1201 aaattattta cggataaagt caccagaggt ggaaaaatga aaaaatggat gttagccatc
     1261 tgcctgatgt ttataaatga gatctgccag gc
//
LOCUS       DAZGXZ010000346         1278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000346 DAZGXZ010000000
VERSION     DAZGXZ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.348"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..85
                     /locus_tag="ACHKJF_005549"
     CDS             <1..85
                     /locus_tag="ACHKJF_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952696.1"
                     /translation="VIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(121..1263)
                     /locus_tag="ACHKJF_005550"
     CDS             complement(121..1263)
                     /locus_tag="ACHKJF_005550"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFP0952697.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          399 a          255 c          214 g          410 t
ORIGIN      
        1 tgttatccgt ggaaacgtag aacaaattga accaaattta ttaggcgttt acgccggctt
       61 attattagcg atgaaaaaca agtaaaaaat aaaaaaggct gcataatgcg gcctttttat
      121 ttattcaaac caattatgaa taacctcttc gaatctttga gttacgccat cccaactgta
      181 gtgttcaaac acaaagtcct gaccttgctt tgcaactgct gttaattcag gattcgctaa
      241 cgtttttaaa atatcgctgc ttatggagtc tgcagtcata ggttctttta aatggaatcc
      301 cgtagtattt tctttgacaa attccgtcat ccccccacgc gtactcacta aaacaggctt
      361 accagcaccc atagcttcaa ttgccaccat gcaaaatggt tcctggaact gggaaggaat
      421 gacgactaaa tctgccagtg ggtaataaca atgcatcttt tcaggcggta tgcttcctaa
      481 cataatgcaa cgatccttca aacgttttgc aatttccctg acattgcgtt gatatgcacc
      541 tttgtcactt ttactcattt ctgtataatc accaatcaca actaatttta aatttttgtg
      601 agcagcggcc aatttttcga atgattgcat caacagcaat atgcctttgt cagggacgat
      661 acgcccagca taaaaaataa ttttctcttc aggggagata ttaagttcgc ttctagtgat
      721 cggctgaaaa ttagattgat aggtttctaa atcaattcca tttggtacaa tctcaatatc
      781 tgcatttgct aaataagatt ggtaatactt tttcaagtat aaactaggca ctatcatttt
      841 aacattttgc tcaagcaatt ttggttcaaa tgcattatgc atatgtataa ccactcttgc
      901 ctgaggagct cgcttacgaa tttgagtgta tagcttcatg ctattatgaa ctacaataac
      961 actttctttc gttataggaa aatcatgtgc aatattcaat attctctgtg aatatggaag
     1021 aggatcaagt cttgtccatt tttgaaataa ccgcttatat atgcgactga atccaaccct
     1081 atgaatacta caattatcat taacaaaggt gtaatttgag tatcctggat ttttgataca
     1141 aacaatacga tttggaaaat tggtacgctg agcaacctga tacatccacg tttcaacagc
     1201 agccgctccg cgaggaggaa ttgaaaaaat aggagtaacc gtaaatatta ttttatcaac
     1261 catctcgtat attaacct
//
LOCUS       DAZGXZ010000347         1260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000347 DAZGXZ010000000
VERSION     DAZGXZ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.349"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            63..>1260
                     /locus_tag="ACHKJF_005551"
     CDS             join(63..384,384..>1260)
                     /locus_tag="ACHKJF_005551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0952698.1"
                     /translation="MAGVLLGKEVRKRKTPQEKIAIIQQTMEPGMTVSHVARLHGIQP
                     SLLFKWKKQYLEGSLTAVAAGEDVVPASELAAAIKQINQLQRLLGKKTMEVEILKEAV
                     EYAQSPKMDSACALVTFGQGIAPVSRVLGVSRAQLTLRMKASDNKPDKRRQRRDEAAD
                     AEVLSRILDIIGDMPAYGYRRVWAILRRQSRNEGLPFVNAKRVYRIMSENSLLLLHDK
                     PSRPQREHKGRISVKESDQRWCSDGFEFGCDDGEKVRVTFALDCCDREAIDWAASTGG
                     YDKATVQDVMLGAIEKRFGDKVPEEPIQWLTDNGSAYRAHETRQFARELNLEPCTTAV
                     SSPQSNGMAERFVKTMKEDYIAFMPKPNVRTAVHNLAVAIEHYNENHPHSALGYRSPR
                     EYRRQRVT"
BASE COUNT          350 a          272 c          364 g          274 t
ORIGIN      
        1 agatgtatct taaaataaga ggtaggcttg gatatatgtc tgacactggt tctaattttg
       61 agatggctgg cgttttgtta ggaaaagagg tgcgtaaacg taaaacacct caggaaaaaa
      121 tcgcaattat tcaacagact atggagcctg gcatgacggt gtcccacgtg gcacgtttgc
      181 atggcattca gcccagcctg ttgtttaagt ggaaaaaaca atacctggaa ggaagcctca
      241 ctgctgttgc tgccggtgaa gacgttgttc ctgcttctga actggccgct gcgataaagc
      301 agattaatca gcttcagcgt ttactgggca agaaaacaat ggaagttgag atcctcaaag
      361 aagcggtgga gtacgcacaa tcgcgaaaat ggatagcgca tgtgcccttg ttaccttcgg
      421 acaaggaata gctccggtaa gccgtgttct gggcgtgtcg cgtgcgcagc taacattacg
      481 gatgaaggca tctgataaca agccagataa acggcgtcag agacgcgatg aggctgcgga
      541 tgcagaagtc ttatcgcgca tcctggatat cattggcgat atgccagcat atgggtatcg
      601 ccgtgtctgg gcgatactca gacggcagtc ccgaaatgaa ggcttgccgt tcgtgaatgc
      661 aaagcgcgtt taccgaataa tgagcgaaaa cagcctgttg ttgttacatg acaaaccatc
      721 acggccgcag cgggaacata aaggccgaat atcggtgaag gaaagcgacc agcgttggtg
      781 ttctgatggc ttcgagttcg gttgtgatga tggcgaaaaa gtccgggtta cgttcgccct
      841 ggactgttgt gatcgtgaag ccatcgactg ggcagccagt acgggaggtt acgataaagc
      901 gacagtacag gatgtgatgt taggcgcgat agaaaaacgc tttggcgata aagtgccgga
      961 agagcccatc cagtggctga cagataacgg ttcagcgtac agagcgcatg aaacacggca
     1021 gttcgccaga gagctgaatc tggagccctg cacgacggca gtcagcagtc ctcagagtaa
     1081 tggaatggca gaacggttcg tgaaaacgat gaaggaagac tacatcgcgt tcatgccgaa
     1141 accgaatgta agaacggcag tgcataacct tgcagtggcg atcgagcatt acaatgaaaa
     1201 ccatccgcac agtgcgttgg gttatcgctc tccgcgagaa tatcgacgtc agcgggtaac
//
LOCUS       DAZGXZ010000348         1259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000348 DAZGXZ010000000
VERSION     DAZGXZ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.350"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..209)
                     /locus_tag="ACHKJF_005552"
     CDS             complement(<1..209)
                     /locus_tag="ACHKJF_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952699.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEE"
     gene            403..828
                     /gene="yqeK"
                     /locus_tag="ACHKJF_005553"
     CDS             403..828
                     /gene="yqeK"
                     /locus_tag="ACHKJF_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
                     /protein_id="HFP0952700.1"
                     /translation="MDIEFSQIHEMAYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFESWICTRCC"
     gene            864..944
                     /locus_tag="ACHKJF_005554"
     CDS             864..944
                     /locus_tag="ACHKJF_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952701.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(977..>1259)
                     /gene="yqeJ"
                     /locus_tag="ACHKJF_005555"
     CDS             complement(977..>1259)
                     /gene="yqeJ"
                     /locus_tag="ACHKJF_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFP0952702.1"
                     /translation="ALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTSKKVNKFEIM
                     DQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR"
BASE COUNT          385 a          202 c          213 g          459 t
ORIGIN      
        1 atctcttcag aaatatccgc tatctttatt gacaaatcat gtgttagata ttttcctcca
       61 agagattctt gttcaatata atttaaacca gaccccattg tgattttaac tggattaacg
      121 gatttctctt catttttctt atttttgttc tcctgatcag gaactaatgg ttgcagtggt
      181 aggctattat taatatttcg tggcttcatt ttacatttct ctcatttgat tagagcaaaa
      241 tccgaattcg cagcttctga taatgtcaaa tatcacctta tggagacgtt acgtagaatg
      301 actatatttg ttatcggcac attaggggta ttaattttag tattgatgga aggtgattat
      361 tgaacagtaa catcgagtca cggtaaaaat gtgaggtata taatggacat tgagttttcg
      421 cagattcatg aaatggccta tatgcatgat attgtaaatt cggactcgaa aaagaaacca
      481 aggattccgt taaaaaaatt ccttaatgct gagaaggttc tcacgcaaac aacatcatgg
      541 gcattaaatt caagatttgt gaatgttaat tctgtcaata aagtcaacgt aaagagtaaa
      601 gttaaaagca gttatatctc acgatctgtc aatgatgagt ttagtttaac agatgatgaa
      661 atcaattctt ttaaagaaac gttagtcctt tcctctattg attcactgag taaactggtt
      721 cttaataatc ctctttctgt attgtttaca tcgactgtac gaaggaataa taatcgagct
      781 aagatgaatg tcgagttcga gtcatggatt tgcaccagat gttgttgata catgatattt
      841 tacattgcaa ggagatgtct aaaatgaaag atgttgatca aaactttgat gctttagact
      901 gccatatact gcgagaatat ttaattttat tgttttatga ttaacgaggt tccatctttt
      961 tgttatctat tttagtttac cttgcacaat gcattattgc tctgttacca atgctcaaaa
     1021 taaaagcatg cttaccttgc tttagtatat tatagcttac acttttttct ggaaagacat
     1081 aaaaatcagg aataagttgt gcaagtctat catcatcaac ttgatccata atctcaaact
     1141 tattaacttt ctttgatgtt aaatgggctg tgtcatagtt ttcaatccat gtgtattcaa
     1201 tatttcgccg tattgattta ctttctctgt taccttgttg ccaggttcca ctcaacgca
//
LOCUS       DAZGXZ010000349         1254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000349 DAZGXZ010000000
VERSION     DAZGXZ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.351"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHKJF_005556"
     CDS             complement(<1..899)
                     /gene="tssI"
                     /locus_tag="ACHKJF_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP0952703.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGR"
BASE COUNT          319 a          317 c          318 g          300 t
ORIGIN      
        1 cgtcccggcc atatctccgg cgagcggctc attccccgcg cggtttctgc gttgtttcgc
       61 cagccgtcca tctgccagcg ggcaaagttc tgcccgtggg cgctcttgaa acgtccgggg
      121 tagtcataca cttcatactg cgtgcgctgg tagtcctggt gctggccttc ctgttcaaaa
      181 cgtccggccc agccggggcg tttaaaggtg tagtctttgg tcaccacgga agaagggcgg
      241 atttgtgcgc tgtagcggaa ctggctgatg cagagggtgc tcacctcggt acgggtgttc
      301 gggttccatg ggatttcaaa agattcgggc agatggcgga ctgtgtcgca cagcaccagg
      361 ctctggtcgg tacttttgta agcatgctcc tcataaaaga agatgccttc ctccgccgcc
      421 atccggcaca gaaaatcgta atcagtctca ccgtactgga cacaaaactc acgggaagga
      481 tgcggctcgc tgaacagcgg actccattcg gtcaccccgt tttcctgcaa catcgtgccg
      541 aggatgcttt tgatgtcctc gttctggaag atacggaagt tctggcgcag accggcacgc
      601 cacagcggcg ggtgcacctt catgctgtac agcatctggt ttttgtcgtt ctccccgagt
      661 tcaaaccagg tcaccacgcc tttcacccgg cgctgtactt catcgccctg ccatatcgtc
      721 aggtaggcca ttttgtccag cacctgcgca aattcaaggg agagaaactg ctggctgacc
      781 agggagagat cgagggaaaa gagcgaagag agtgactggt tcagatgaaa ggaaaccacc
      841 gcaaaagcat ccggcggcag gccgtccact tccagtgtga aacgtaatcc ggttgacata
      901 gcaacctccg gtaaaatgaa taatgatccg taccgtacaa ctgcggataa tgtgataagc
      961 gggacgactt cagacgttat tagggaagga acagaaaatg ctatggttat gtgagaattt
     1021 tgtttaaata ttactttaca gaaacgggtg tctgttcagt caaaaaaata atcacaagct
     1081 caacctcttg tttcttggcg ttattcagga caggtacgca tatcacaacg agtaacaaaa
     1141 acgcaaagtt aaaccaatat tcataatata tttttattca cagcaataaa taatccaatt
     1201 aacattatta aaatgaatga ttgcataaga aatatatgct accgctccac cctg
//
LOCUS       DAZGXZ010000350         1248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000350 DAZGXZ010000000
VERSION     DAZGXZ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.352"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            74..>1248
                     /locus_tag="ACHKJF_005557"
     CDS             74..>1248
                     /locus_tag="ACHKJF_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0952704.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRPSIKRDLVDYNIINELLFNCSKIQPVENKKDANETLELKIIELQMKINNL"
BASE COUNT          439 a          182 c          260 g          367 t
ORIGIN      
        1 tttaaataaa aattaaacaa aaattatatc ccaccactaa ggtttataaa agcatacgtt
       61 agcaggtgtc accatgaaaa aagccatagc atatatgcga ttttcatcac caggtcagat
      121 gtctggtgac tcattaaacc gacagagaag acttattgct gaatggttaa aggtaaatag
      181 tgattattat cttgatacca taacatatga agatttagga ttaagtgcat tcaaaggaaa
      241 gcatgcacaa tcaggagctt tttcggaatt tttagatgct atagagcatg gttatatatt
      301 gccaggaact acattgttag ttgaaagtct ggacagactt tcaagagaaa aagtcggtga
      361 agcgattgaa cgtctgaaat taattttgaa tcacggtatt gatgttataa ctctttgcga
      421 caatacagtc tataatattg actctttgaa tgagccatat tcattaataa aagccatact
      481 tatagcacaa agggcaaatg aagaaagcga gataaagtca agtcgggtta aattatcatg
      541 gaagaaaaaa cggcaggatg cactggaatc aggtacgatt atgacggcgt cttgtccgag
      601 atggctctcc ttagatgaca aaagaacggc ttttgttcca gaccccgaca gggtgaaaac
      661 tattgagcta atttttaaac tcaggatgga aaggcgctca ttgaatgcaa tagccaagta
      721 tttaaatgat catgctgtaa agaatttctc aggaaaagaa agtgcatggg gaccttctgt
      781 aattgaaaaa ttattagcga ataaagctct gataggtata tgcgtacctt catatcgtgc
      841 aagagggaaa gggataagtg aaatcgctgg ctattatccc agagtcatat cagatgattt
      901 gttttacgct gtacaggaaa ttcggttggc accttttggt attagcaata gtagcaagaa
      961 tcctatgcta ataaatctac ttcgaacagt tatgaagtgt gaggcttgtg gtaataccat
     1021 gattgttcat gcggtatctg gaagtttgca tggctattat gtttgtccga tgagaagatt
     1081 acatcgatgt gacaggccat caataaaaag agatttggtt gattataata tcattaatga
     1141 attgcttttt aattgtagca aaattcaacc agttgaaaac aagaaagatg ctaatgaaac
     1201 tttagagtta aaaattattg agcttcagat gaaaattaat aatttaat
//
LOCUS       DAZGXZ010000351         1247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000351 DAZGXZ010000000
VERSION     DAZGXZ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.353"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..254
                     /locus_tag="ACHKJF_005558"
     CDS             <1..254
                     /locus_tag="ACHKJF_005558"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952705.1"
                     /translation="ENGACAYDAEPRAVPAFPALAHAPTAVELVSDAEAVPGDAQELS
                     SCANAGAPAIRKEISDKVIPCRFIFINNLIDIHNILAQS"
     gene            complement(479..>1247)
                     /locus_tag="ACHKJF_005559"
     CDS             complement(479..>1247)
                     /locus_tag="ACHKJF_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952706.1"
                     /translation="NDKIEPGKGANRVDGGSGYDTLLLSDVMDDWNIYRVSDGTLFFH
                     SERGAGLTEATGIEGISFSGDWRSKLSPYQIEEEQITDHRYLLKWRNKNVDYGKHTEG
                     TNGDDSLFGNIIFGLDGNDILHSAGGEGVSLLHGGRGNDYLHGASGNDELYGAEGNDW
                     LYGGGGNDYLYGGTGHDVFVFDAACEGNSFIMDFNKHKGEKDFLFLSKDIVTSTDEFI
                     SCLKQTGNDVEAGIAGGRIYFVNTALEDINHNSVYIV"
BASE COUNT          408 a          326 c          191 g          322 t
ORIGIN      
        1 tggaaaacgg cgcatgcgca tacgatgcag agccaagagc tgtccctgcc tttccagccc
       61 tcgcccatgc acctacagcc gtggagctgg tctctgatgc agaggctgtg cccggagacg
      121 cccaggaact gtcatcctgc gcaaacgccg gagcaccggc aatcagaaag gaaataagcg
      181 ataaagtaat accatgtcga tttattttca taaacaacct cattgatata cacaatatac
      241 tggcacagag ttaaattaat atataatcag gagagaagtg aaagagtacc cggtgcgaaa
      301 atgaattaat ctgagcagat ttaacggcaa acgaagcgca ataaattaaa attaaaaaaa
      361 caaaatcact tgcagtctat tatctttacg ttcctcaccg caaccgcaca caaaaacaaa
      421 acacacaatg cattgatttt aaacaaaaaa aacaaaacag aaactcaaca cataaaaatc
      481 agacaatata aacagaattg tgatttatat cctcaagcgc tgtattaacg aaatatatcc
      541 gtcctcctgc aatacctgcc tcaacatcat ttccggtttg tttcagacat gaaatgaact
      601 catctgtcga tgtaacaatg tcctttgata aaaacagaaa atctttttca cccttatgtt
      661 tattaaaatc catgataaat gaattccctt cacaagcagc atcaaagacg aagacatcat
      721 gaccagtacc accatacaga taatcattac ctcccccacc atataaccag tcgttacctt
      781 ctgctccgta caactcatca ttaccagaag ccccatgaag ataatcattc ccgcgacctc
      841 cgtgtaaaag acttactcct tctcccccgg cactatgtaa tatatcattt ccgtcaagac
      901 caaaaataat atttccgaac agactatcat ctccgtttgt accttctgta tgttttccgt
      961 aatcaacatt tttattacgc cacttcagaa gatacctgtg gtctgtaatc tgctcttctt
     1021 ctatctgata agggcttagt ttactcctcc agtcaccaga aaaagagata ccttcaatac
     1081 ctgttgcctc agtcagaccg gctccccgtt ctgaatggaa aaacagcgtc ccatcactga
     1141 ccctgtatat attccagtca tccattacat cgctaagaag cagcgtgtca tacccactgc
     1201 caccatcaac cctgtttgct cccttacctg gttcaatctt atcattc
//
LOCUS       DAZGXZ010000352         1244 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000352 DAZGXZ010000000
VERSION     DAZGXZ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.354"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            26..1239
                     /locus_tag="ACHKJF_005560"
     CDS             join(26..317,317..1239)
                     /locus_tag="ACHKJF_005560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0952707.1"
                     /translation="MTKNTRFSPEVRQRAVRMVIESQNDYDSQWAAISSIAPKIGCTP
                     ETLRTWLRQYERDAGAGDGGLSTTERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPVEKIAPLVDTLRGAHGVGPVCHELAVAPSTYYRHRAHQRHPDKRSQRAQRDDLLK
                     PEIQRVYDENHSVYGVRKVWRQLLREGVTVARCTVERLMKVMGLAGVRRGKKVRTTVS
                     RKEAAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFAYVAFIIDVFAGVIVGWRVSSS
                     MKTTFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKDAELLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEKA
                     YYASIGNGGLAA"
     misc_feature    307..423
                     /locus_tag="ACHKJF_005560"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          276 a          328 c          406 g          234 t
ORIGIN      
        1 actccttgag agagaggtaa acaggatgac taaaaatacg cgcttttccc ctgaggtccg
       61 tcagcgggcc gttcgtatgg ttatcgaaag ccagaatgac tatgactcgc agtgggcggc
      121 catcagctcc atcgccccta aaatcggctg tacgcctgag accctgcgta cctggctgcg
      181 tcagtatgag cgcgatgccg gtgcaggtga cggcggactg agcaccactg aacgtcagcg
      241 tctgaaggaa ctggagcgtg aaaaccgcga actgcgccgc agcaacgata tcctgcgcca
      301 ggcttccgcg tattttgcga aggcggagtt cgaccgcctg tggaaaaaat agcgcctctg
      361 gtggataccc tgcgtggtgc gcacggggtc ggaccggtat gccacgagct ggctgtcgcc
      421 ccgtcaacgt attaccggca tcgtgcgcat caacggcatc cggataaacg cagtcagcgg
      481 gcacagcgtg atgacctgct gaagccggag atacagcgcg tgtacgatga aaaccacagc
      541 gtgtacggtg tgcgcaaggt ctggcgccag ttgttgcgtg agggggtgac cgtggccagg
      601 tgcacagtag aacggctgat gaaggtgatg gggcttgccg gtgtgcgccg gggtaaaaag
      661 gtccgcacca cagtcagccg gaaagaagcc gctgcgggtg accgggtgaa ccgccagttc
      721 gtggcagaac gccccgacca gctgtgggtg gctgacttca cgtatgtgag cacctggcag
      781 ggcttcgctt acgtggcgtt catcatcgat gtgttcgccg gcgtcattgt gggctggcgg
      841 gtctcatcgt cgatgaaaac gacgttcgtg ctggatgcac tggagcaggc gctgtgggcc
      901 cgtcgcccgt cgggcaccat ccatcactca gataaggggt cacaatatgt gtcgctggca
      961 tacactcagc ggcttaagga tgcagagttg ctggcgtcaa ccggcagcac aggcgactca
     1021 tatgacaacg cgatggcgga gagcatcaac ggactttata aagcggaggt gatacatcgt
     1081 aagagctgga aaaaccgcgc cgaagtggaa ctggccacac tgacgtgggt ggactggtac
     1141 aataaccgac ggttgctgga acgactgggt catatcccgc cggcagaagc ggaaaaggca
     1201 tattacgctt ccatcggaaa tggtggtctg gcagcctgag ttca
//
LOCUS       DAZGXZ010000353         1238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000353 DAZGXZ010000000
VERSION     DAZGXZ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.355"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            36..>1238
                     /locus_tag="ACHKJF_005561"
     CDS             36..>1238
                     /locus_tag="ACHKJF_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YeeR"
                     /protein_id="HFP0952708.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEK"
BASE COUNT          302 a          303 c          310 g          323 t
ORIGIN      
        1 attaacaata acatgatatt catcacggag tgactatgtt acagatagtc ggcgcgctga
       61 tcctgctgat cgcaggattt gccattcttc gccttttgtt cagagcatta atcagcacgg
      121 cttctgcgct ggcagggctc atattgctgt gtctgttcgg cccggcctta ctggctggct
      181 atatcaccga acgcataacc cggttgttcc atattcgctg gctggcaggc gtatttctga
      241 cgattgccgg aatgatcatc agcttcatgt ggggacttga tggtaaacat atcgcgctgg
      301 aggctcacac ctttgactct gtgaaattta ttctgaccac cgctctcgcc ggtggtctgc
      361 tggctgttcc cctgcagatc aaaaacattc agcagaacgg gatcacacca gaagatatca
      421 gcaaggaaat taacgggtat tactgctgtt tttatactgc ctttttcctt atggcgtgtt
      481 ctgcatgcgc accattgatc gcgttacagt acgatatttc accgtcactg atgtggtggg
      541 gcgggttgtt gtactggctg gctgcattag tgacgctgct atgggcggcc agccagatcc
      601 aggcgctgaa aaaactgacc tgtgccatca gccagacact ggaagaacaa ccggtgctca
      661 acagtaaatc gtggctgacc agtttgcaaa acgattacag ccttcctgac tcactgacgg
      721 agcgcatctg gctgacgctc atttctcaac ggatttcccg gggagagctg agggaatttg
      781 aactggcaga cggaaactgg ttactgaaca atgcctggta tgaaagaaac atggcagggt
      841 ttaacgaaca gttgaaagag aacctgtcat tcacacctga tgaactgaaa acgctcttcc
      901 ggaaccgcct gaatttatca ccggaagcga atgacgattt tctcgatcgt tgcctggacg
      961 gcggtgactg gtatcccttt tcagaaggtc gccgttttgt atcattccat cacgtggatg
     1021 agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca tcatgccccg gaaaatcata
     1081 agccggatcc ggaatggtac tgctcctctc tttgtcgcga aacagaaaca ctgtgtcagg
     1141 aaatttatga acgcccttac aacagcttta tttccgatgc aacggcgaat ggtctgattc
     1201 tcatgaaact gccggaaacc tggagtacaa atgagaaa
//
LOCUS       DAZGXZ010000354         1235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000354 DAZGXZ010000000
VERSION     DAZGXZ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.356"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..1155)
                     /gene="espL4"
                     /locus_tag="ACHKJF_005562"
     CDS             complement(<1..1155)
                     /gene="espL4"
                     /locus_tag="ACHKJF_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFP0952709.1"
                     /translation="MITRIPRSSFSANINNTAQTNEHQTLSELFYKELEDKFSGKELA
                     TPLLKSFSENCRQNGRHIFSNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPI
                     FEEYLMEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAA
                     QYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGL
                     VMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKI
                     RIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDE
                     ITPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNV"
BASE COUNT          348 a          214 c          235 g          438 t
ORIGIN      
        1 aacgttcata tgtccactat tataggcagt caacatttct gttctatgta gttctggcat
       61 tttctggagc tgaatcatca gttgcgtaag ttggtgattc tgaccaattg ccattagcaa
      121 attcatcgtt gctggtgata atggaggggt tatttcatcc acagatcctt ttaacatcaa
      181 tagctgttct ttcggaagtg ctggaattat tgcaacagat tggttaataa catgatcatt
      241 tgaccatttc aggaggctat aatctacatt cataaaatcc atcagagaat agtgtttaat
      301 atcctctttg ctttcgctca taattcttat tttatcgtta gttacattag gatcataaac
      361 cgaaaccacg taatgggtac atccttcagt tgtgttcttt attcttagcc ggaccgtcaa
      421 agcatggtta tccaccagta atatggctgc cataacactg atgccatttg attccatctt
      481 gtcaaaatat gctttcataa ctagcccgaa atcaccgcag gcagcaacgc aacgcccctc
      541 aggacggctg gaaaccaacg ccaaagtctg gtttttgcat tttttgttga agtgcgtgag
      601 aaatttttca gctgaagagt aatcatcttg atggggtttg taatcaacat tctgataaat
      661 atcattaata tattgtgcag caatgtgtcg gcatactata cggttttggg gtcgccccgg
      721 aaaataacat tgaccattaa gtttaattct tgggaattct ttttcttttc tatctgattt
      781 taggtttaat tcttgtttat caagagtgtc tgagcgttga ggtaaaattt ccattaggta
      841 ttcttcaaat attggggcaa tggtttgagt aagtgtcgtg ttttcgtttt tattaattat
      901 cgtaatttct ttcttatcag cttgtaagac ggacgtggaa aatttaatga caaaatcctt
      961 gttgctaaag atatgacgac cattttgtct acagttctct gagaagcttt ttaatagagg
     1021 cgtcgccagc tccttgccag aaaatttatc ctcgagttct ttataaaaca attcactcag
     1081 ggtttggtgt tcatttgtct gggctgtatt attaatattt gcagagaaag aactacgagg
     1141 gatacgagta atcatttaaa tatctcattg tttattgatg tcttgaattt taactatcag
     1201 aacagtgaaa aaatttaata tgattacaag taaag
//
LOCUS       DAZGXZ010000355         1199 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000355 DAZGXZ010000000
VERSION     DAZGXZ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.357"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1199
                     /locus_tag="ACHKJF_005563"
     CDS             <1..>1199
                     /locus_tag="ACHKJF_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0952710.1"
                     /translation="GKSASATIAVNAGKGGLTDAAGTTKTRLTYTPDGKSPAEELATM
                     NDGLSFEGDDGKIIHKKLNEKLTIKGGAGDNEDVTDSNIRVDANEKGDGLLVRMTRNL
                     RDLASAVFGTENGPTTRIDNAGITITTPAAGGGQGKTVSLTGSGLNNGGNQITSVASG
                     LNGTALADASGDVLNNAANIGDLKNALRDSSAALVNTGFGLQADDGSKVNTSLGKHIT
                     VGGDGQNISTTVQNGSLTVRLADNIRLADDGSVSVGGTRMDKNGLTIKDGPSVTVNGI
                     DAAGRVISGVKDGVNDQDAVNVSQLNREISQATAATTWALKTESGDEAVAVSSQTVEV
                     RHGQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQGNPLVNIGGRFYTQTEDAATGK
                     LTMTPAEP"
BASE COUNT          325 a          272 c          353 g          249 t
ORIGIN      
        1 ggaaagtctg cgtcagcaac cattgctgtt aatgcaggca aaggtggatt aacagatgct
       61 gccggtacca cgaagacacg tctgacttat accccggatg gaaaatcacc agcagaagag
      121 ctggcgacca tgaatgacgg tctttctttt gagggtgatg acggaaagat tattcataaa
      181 aaactgaatg aaaaactgac catcaaaggt ggtgctggtg ataatgagga tgtaacagac
      241 agtaacattc gtgttgatgc aaatgaaaag ggagatggtc ttctcgtccg gatgaccaga
      301 aacctccgtg atttggccag cgcggttttt ggtacagaaa acggcccgac aacccggatt
      361 gacaatgcag gcatcacaat cacaacacct gctgcgggcg gtggtcaggg gaaaacagtt
      421 tcccttaccg gctccggcct gaataacggt ggaaatcaga taaccagtgt ggccagtggt
      481 ctgaacggca ctgctcttgc tgacgccagt ggagatgttc tgaataatgc ggccaatatc
      541 ggtgacctga aaaatgctct ccgtgacagc agtgcggcac tggtgaatac cggctttggt
      601 ctgcaggcgg atgacggaag caaggtgaac acgtctctcg ggaagcatat cacggttggt
      661 ggtgacgggc agaatatcag cacgactgtt cagaacggga gcctgacggt cagactcgct
      721 gataacattc gcctggcaga tgacggcagt gtttctgttg gcggaacccg gatggataaa
      781 aacggactga ccatcaagga tggcccgtca gtgactgtga acggtattga tgctgctggc
      841 cgggttattt ccggcgtgaa agacggggtg aatgaccagg acgccgttaa cgtttcacaa
      901 ctgaaccgtg aaatcagtca ggcaacggca gcaacgacct gggcactgaa gactgaatcc
      961 ggtgatgagg ctgttgctgt ttcctcccag acggttgaag tccgtcacgg acagaatacc
     1021 cgggtttcag ccatcagtaa ggatgacaag ggaaattaca gctatgagat tgatgtgacg
     1081 gggattccgg tggagtacac cgacagccag gggaacccgc ttgtcaatat tggaggtcgt
     1141 ttttacacac agaccgaaga cgctgcgacc gggaaactga caatgacgcc ggctgaacc
//
LOCUS       DAZGXZ010000356         1193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000356 DAZGXZ010000000
VERSION     DAZGXZ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.358"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1193
                     /locus_tag="ACHKJF_005564"
     CDS             <1..>1193
                     /locus_tag="ACHKJF_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0952711.1"
                     /translation="GKSASATIAVNAGKGGLTDAAGTTKTRLTYTPDGKSPAEELATM
                     NDGLSFEGDDGKVIHKKLNEKLTIKGGAEKNAEVTDGNIRVDNDNGGLLVRMTRNLRD
                     LASAVFGTENGPTTRIDNAGITITTPAAGGGQGNTVSLTGSGLNNGGNQITSVASGLN
                     GTALADASGDVLNNAANIGDLKNALRDSSAALVNTGFGLQADDGSKVNTSLGKHITVG
                     GDGQNISTTVQNGSLTVRLADNIRLGDDGSVSVGGTRMDKNGLTIKDGPSVTVNGIDA
                     AGRVISGVKDGVNDQDAVNVSQLNREISQATAATTWALKTESGDEAVAVSSQTVEVRH
                     GQNTRVSAISKDDKGNYSYEIDVTGIPVEYTDSQGNPLVNIGGRFYTQTEDAATGKLT
                     MTPAEP"
BASE COUNT          316 a          270 c          362 g          245 t
ORIGIN      
        1 ggaaagtctg cgtcagcaac cattgctgtt aatgcaggca aaggtggatt aacagatgct
       61 gccggtacca cgaagacacg tctgacttat accccggatg gaaaatcacc ggcagaagag
      121 ctggcgacca tgaatgacgg tctttctttt gagggtgatg acggaaaggt tattcataaa
      181 aaactgaatg aaaaactgac catcaaaggt ggtgcagaga agaatgctga agtgactgac
      241 ggcaatatcc gggttgataa tgataatggc gggcttctgg tgaggatgac cagaaacctc
      301 cgtgatttgg ccagcgcggt ttttggtaca gaaaacggcc cgacaacccg gattgacaat
      361 gcaggcatca caatcacaac acctgctgcg ggcggtggtc aggggaatac ggtttccctt
      421 actggctccg gcctgaataa cggtggaaat cagataacca gtgtggccag tggtctgaac
      481 ggcactgcgc ttgctgacgc cagtggagat gttctgaata atgcggccaa tatcggtgac
      541 ctgaaaaatg ctctccgtga cagcagtgcg gcactggtga ataccggctt tggtctgcag
      601 gcggatgacg gaagcaaggt gaacacgtct ctcgggaagc atatcacggt tggtggtgac
      661 gggcagaata tcagcacgac tgttcagaac gggagcctga cggtcagact cgctgataac
      721 attcgcctgg gagatgacgg cagcgtttct gtcggcggaa cccggatgga taaaaacggg
      781 ctgaccatca aggatggccc gtcagtgact gtgaacggta ttgatgcggc tggccgggtt
      841 atttccggcg tgaaagacgg ggtgaatgac caggacgccg ttaacgtttc acaactgaac
      901 cgtgaaatca gtcaggcaac ggcagcaacg acctgggcac tgaagactga atccggtgat
      961 gaggctgttg ctgtttcttc ccagacagtt gaagtccgcc acggacagaa tacccgggtt
     1021 tcagccatca gtaaggatga caagggaaat tacagctatg agattgatgt gacggggatt
     1081 ccggtggagt acaccgacag ccaggggaac ccgcttgtca atattggtgg tcgtttttac
     1141 acacagaccg aagatgctgc gaccgggaaa ctgacaatga cgccggctga gcc
//
LOCUS       DAZGXZ010000357         1187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000357 DAZGXZ010000000
VERSION     DAZGXZ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.359"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..1108
                     /locus_tag="ACHKJF_005565"
                     /pseudo
     CDS             1..1108
                     /locus_tag="ACHKJF_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          465 a          230 c          175 g          317 t
ORIGIN      
        1 tgactttacc ccaggtataa taaatacata tcacggcgat atttataact gcacaacaaa
       61 taccgataat gttaaaacgc cagacacacc gaagtggcct tgtgataatt gggaagaaca
      121 acaacccatt aattccatat tctctggaga aggatacaac cctgaacaat ttgatttagc
      181 gcaacaacaa ctctaacaaa ttaatgcatg ccacacgaac accacatata caaatgcaga
      241 ctactcaaaa gttgtggttc aacttgtaag tcttattaat aacattgaaa cgataagttc
      301 gacaccactt acgcaacaga cacaatcaat acttaatcag attaataaca ttagatatga
      361 aaaaaacaaa agtgctgaat gtcgaatcat tgttgtcgct aatcctaaac ccgacaaggc
      421 aatcataact aaaattagcg tagaagaggg aatacccata acattcagtg tccaaaccat
      481 gttttcagat actaatttta ttgctgaaca acgagctgac ttaccaacta atataaaaga
      541 tattcaaagc ctgtatcaaa aaatgacgaa gttatatatt gagcacagtg aaaataaaaa
      601 caggatgaaa gtctttgccg gaaccaactt tattgatttc aatatgacag gacagaacct
      661 gtccgggttc gttctaacct tgtcaaggtt ttatttcgaa gatctactta atataaattt
      721 cactgacgca aatttaggtg acactatttt ttacataaag aacaccctac ccccaaatta
      781 tataaagatg gacaatatct tgacaaacaa atcgaaggtt tattttcaac attattaacc
      841 attaatgaca actttttacg agcaaaagcg gaaattgctt cgacaataat taaattttta
      901 gaggctagga taaccaatct ttcatacaat gacatattaa catatcagca agaattccaa
      961 aaacaatgtt ataagcaagt taaagccttt gcgacactct ctcgatacaa taaaattcaa
     1021 acatgggcag aaatgtctga atatcagttc gaagtatttc aatacgagac gcttaacccc
     1081 aaaaaaatgt cacacacgcc ttatttaaag aggccacttc ctaatgaaaa agatataaat
     1141 tatggggtgg aaatagaaat accatctggc aaaagaattc gcttatc
//
LOCUS       DAZGXZ010000358         1164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000358 DAZGXZ010000000
VERSION     DAZGXZ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.360"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            10..225
                     /gene="traY"
                     /locus_tag="ACHKJF_005566"
     CDS             10..225
                     /gene="traY"
                     /locus_tag="ACHKJF_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFP0952712.1"
                     /translation="MIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVRLRDH
                     LSRFPDFYNLDAIKEGTGETDSIIKDL"
     gene            258..623
                     /gene="traA"
                     /locus_tag="ACHKJF_005567"
     CDS             258..623
                     /gene="traA"
                     /locus_tag="ACHKJF_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFP0952713.1"
                     /translation="MNAILSVQGASAPVKKKSFFPKFTRLNMLRLVRAVIPAAVLMMF
                     FPQLALAAQGAGQDLMAGGNATVKATFGKDSSAVKWVILAEVLVGAIMYMMTKNVKFL
                     AGFAIISVFITVGMAVVGL"
     gene            638..949
                     /gene="traL"
                     /locus_tag="ACHKJF_005568"
     CDS             638..949
                     /gene="traL"
                     /locus_tag="ACHKJF_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFP0952714.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWLGLPLDELIPAGICIGWGMMT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            971..>1164
                     /locus_tag="ACHKJF_005569"
     CDS             971..>1164
                     /locus_tag="ACHKJF_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
                     /protein_id="HFP0952715.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLAVNVIQGINNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSA"
BASE COUNT          290 a          252 c          298 g          324 t
ORIGIN      
        1 agcagaaata taataaggcc tgctccgggc aataaagttt tactggtatt ggacgatgct
       61 actaatcaca agctcttagg tgcccgggag cgtagcggga gaacaaaaac taatgaggtg
      121 ctagtcaggt tacgtgatca tctgagccgt tttcctgact tttataatct ggatgcaata
      181 aaggagggaa caggggaaac tgattcgata attaaagatc tttaagttta ttaatctatc
      241 cacaatagag gtaacttatg aatgctattt tgagtgttca gggtgcttct gcgcccgtca
      301 aaaagaagtc gttttttcct aaattcacgc gtctgaatat gcttcgcctg gttcgtgcag
      361 tgatcccggc tgctgttctg atgatgttct tcccgcaact ggcactggcc gcccaggggg
      421 caggtcagga cctgatggcc gggggtaacg ccaccgtcaa ggccaccttc ggtaaagact
      481 ccagtgcggt gaaatgggtg attctggcgg aagttctcgt cggggccatc atgtacatga
      541 tgaccaaaaa cgttaagttc ctcgccggtt ttgccattat ctccgtgttt attaccgtgg
      601 gtatggcagt cgtcggtctc tgacaggaaa taacgtcatg tcgggagacg agaataaact
      661 caaaaaatat cgtttcccgg aaacactgac caaccagagc cgctggttag gcctgccgct
      721 ggatgaactt attcctgccg gaatttgtat tggctgggga atgatgacat caaaatatct
      781 gtttggtatt ggtgcagcgg ttctggttta tttcgggatt aaaaaactga aaaaagggcg
      841 gggcagctcc tggttacgtg acctgattta ctggtatatg ccaacagccc tgttgcgcgg
      901 tatttttcat aatgttcccg attcgtgttt ccggcagtgg attaaataga actgatacca
      961 ggattattct atggaacacg gtgcccgttt aagtaccagt cgtgtaatgg ccatcgcctt
     1021 tatatttatg tcggtgctta ttgtcctcag cctcgccgtt aacgtcattc aggggataaa
     1081 taactaccgt cttcagaatg agcaacgcac tgccgtgacc ccgatgggat ttaatgtacc
     1141 ctttgccgtg tcacagaaca gtgc
//
LOCUS       DAZGXZ010000359         1160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000359 DAZGXZ010000000
VERSION     DAZGXZ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.361"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            474..623
                     /locus_tag="ACHKJF_005570"
     CDS             474..623
                     /locus_tag="ACHKJF_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFP0952716.1"
                     /translation="MEKVISHTRYKLTPAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
BASE COUNT          316 a          241 c          312 g          291 t
ORIGIN      
        1 gcgctatccc tgatagctgg cgtataacct gcgtatataa aacgtaccag caacgcacgt
       61 gaacagagtg cattaagcat ccgtctgagc ggtaaacgta tccgacctgg tgagtgacag
      121 tctgagagca ttctcagtaa tcgccccctt gtatcctgga aattggttaa gagcgattag
      181 atttggcaag gtgtatgcag ccagcagctt aacacctcag ctaactggct ggttaccttc
      241 aggttgtcgc aatggcatcg gacatccggt gtcggataac taaacgtagc gttaaggcga
      301 tattgctgag agaccggtta agtggtggca cgggagtaat gagtttgggg tgggtaccag
      361 aatgccagtc tggcggtatg ctggcagtat cacgttgatt acagcataaa gtgatgagag
      421 gctaatggaa gttaaaacca aagcagttcc gtcgttgttc atcccgggag acactggaaa
      481 aagtgatttc ccatacgcgt tataaactta cccctgcgga gctggaagcc ttcaactctg
      541 tggttgatca ctggctggca gaactgacga tgaacaagct ttacgatcgt gttcccgctt
      601 cagtctggaa atatgtcatc tgagcgttcg gtaatcacaa ctgcatcgcg ccaggtgtta
      661 tccgctttgt gattcttatt ggctcttctc aggttattaa aagctgaata tgttcctgtt
      721 gtcgggaacc aggataatac cgggaacgtt aagcggtata gcgacaacaa agaattcgaa
      781 atcaaaaggg aatagtgccg gaggcgtggg cggtaatggg ctgattttca ggtattaccg
      841 gcttacagcg tgtggttgat attgaggggc tgtgagtttc acgtatttgt cagaaaccca
      901 gacgagtttt cctgtatggg acgaaataag gcagcggcaa tacatggaaa aatttaatat
      961 ggtatgaagg agaacctgtc ggttacgctt aatataaccg gtacactgtc agctcagggg
     1021 atacatgaac cgttatctca ccagctacac agtatataca cactgacgct gacagtagtc
     1081 agcaggacca caataatcag tgccagcagt atggtaacga tacggtctgt attccggtaa
     1141 tagccgaagg ggtcgacaga
//
LOCUS       DAZGXZ010000360         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000360 DAZGXZ010000000
VERSION     DAZGXZ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.362"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(59..844)
                     /locus_tag="ACHKJF_005571"
     CDS             complement(59..844)
                     /locus_tag="ACHKJF_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0952717.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          337 a          235 c          215 g          372 t
ORIGIN      
        1 ttatgcaaag aattttgtat tattgccgcc agaaattcct ggcggcagat ataatttatt
       61 attgatagcg gttaaccaaa tcttttacca tcgctttata ggtcttttgc ttaagttgca
      121 tgatttgctg ctctgtcata tcagtcgtac tggcttcatt cttgtcgggc aatggtggaa
      181 gcactttata ttcataattc accggcgacc aggtagccca ctcacctgtt gccacatcca
      241 ccagcgtgaa acgtaccagg tagcgcaggg agatagcgtc ggtgagtttt tcatttttat
      301 agtcggacca tactgttgat ttcagcgcag agtcatattt tcctgtctgc aatgtatcct
      361 ggtaaacaat aatcgctttc tgatgtcctt ttgcagccac aaaacgcagt gcctgcatcc
      421 agttcatatt ctcagcactg gcaacatcct cgttctcatt tttatctttg tttttgttac
      481 aagtcagagg cttttgcctg tccgggatac cagagaatgt ggaaacagta taatattttc
      541 gcatctcctc ctgcataatg gtttccggtg cgcggttgcc ggattgaaca agtattacag
      601 gagaattaag gggaacgcgg aactcatctc catcaagcgc agcttgaata tcctcttcgg
      661 atacggccag agtggtttca ttaccgaata aatctttgtc tgtcagttgc tggggagtgt
      721 ttttattaat attactttgt gtgacttgag cttttttctt cttttcatca atagaagatt
      781 cagaggcaca tcctgcaagc agcgagaata acacacatat gcaacaaagc ttttttgaat
      841 tcataattgg acactccctc gcctgtcata atgattagaa tatttagcat tgataacgga
      901 ctctaattat ataccccact taaataataa caaacaaaat caatttgaaa tatacattca
      961 tttaatcaat ataaatttat tctctggaaa tatatttaag ctgaatggtt cgatatcatt
     1021 attgaatttt aatgaatgag acaacaagcg agaatcacgc acttacgcca gaataaaatc
     1081 attgaacaga gaaatggtgg aattcaaata caaaaaaacc gcaaaattaa aaatcttgcg
     1141 gctctctgaa ctcattttc
//
LOCUS       DAZGXZ010000361         1140 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000361 DAZGXZ010000000
VERSION     DAZGXZ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.363"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..966
                     /locus_tag="ACHKJF_005572"
     CDS             <1..966
                     /locus_tag="ACHKJF_005572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP0952718.1"
                     /translation="DVSRSESEAEYYRYKVDEAGEETIRSFVSLYLDALREKQSIAVL
                     EQSLSRHNAILNDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLKTTLGKLST
                     WTTNPVTEADLENPFSRMTESRLLTDFTQTPQKGNPSWLASQADVESKKAALKAQELA
                     RYPRVDLTGSVTRDDRQIGVSLSWDLFNRNASYGVSEKAAQIAAATGRLDSVARMIDE
                     TGRLSLITVRQSRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNAENELYSVG
                     LSRVQTEDQMLHGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            971..>1140
                     /locus_tag="ACHKJF_005573"
     CDS             971..>1140
                     /locus_tag="ACHKJF_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP0952719.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRR"
BASE COUNT          304 a          251 c          316 g          269 t
ORIGIN      
        1 gatgtcagcc ggagtgagtc ggaagccgag tattaccgtt ataaagttga tgaggctggc
       61 gaggaaacaa ttcgctcctt tgtttcatta tatcttgatg cgctaaggga aaagcaatcc
      121 attgcggtac ttgaacagag tctctcccgg cataacgcaa ttcttaatga tcttaacacc
      181 atcagtattc acgataccgg gcgggaatct gaatttgttc aggccgaagc ccgcaggctg
      241 atggtccggc agcagataaa tacccgcacc cgagtgctca aaaccacgct gggaaaactt
      301 tcgacctgga ccacaaatcc ggtaaccgaa gccgatcttg aaaatccttt ttcccggatg
      361 acagagtcca gattgttaac tgattttacg cagacgcctc agaaagggaa tccgtcgtgg
      421 cttgccagtc aggctgatgt agagagtaaa aaagcggcgc tgaaagcgca ggagcttgcc
      481 cggtatcccc gggtggattt aacgggatca gtgacccggg atgacaggca gataggtgtc
      541 agtctgtcct gggacctgtt caaccgtaat gccagttatg gtgtcagtga aaaggccgcg
      601 cagatagctg cagctaccgg acgactggac tctgttgccc gaatgattga tgaaaccgga
      661 cgattatcgc tgataacggt cagacaaagt cggatggaaa tggaaacgct cagacgtcag
      721 gagcaggcct ctgccagagt tgtggacttt tatcgtcttc agttccgggt ggcaagaaaa
      781 acactgattg aattactgaa tgcagaaaac gaactgtaca gcgtcgggct ttcccgggtt
      841 cagacagagg atcagatgct ccacggaatg ctggattatc tgtattccca gggaatgctc
      901 ctgaaatgga gcggagtgaa tctttctggt gcagaagaaa aagtaacagg gcagaaggac
      961 agataaataa gtgaagagta tttttgaagg attatcacag gtaacggcat tactgggtac
     1021 acctgtatct gctgagacgc tggctgccgg tacagtccgg acagatgtct ccgggattga
     1081 ttttcgttct gttggcgagt ttcttcgcag tgaagggttt gataatcatc tttcccggcg
//
LOCUS       DAZGXZ010000362         1133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000362 DAZGXZ010000000
VERSION     DAZGXZ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.364"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..85)
                     /locus_tag="ACHKJF_005574"
     CDS             complement(<1..85)
                     /locus_tag="ACHKJF_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952720.1"
                     /translation="MAPDRTTGRDPPQRREARFAKRRCALHP"
     gene            385..687
                     /locus_tag="ACHKJF_005575"
     CDS             385..687
                     /locus_tag="ACHKJF_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952721.1"
                     /translation="MSAFSFNTAYQPTGDQQKDAIAQIDIMQNRAVQANLAYQSSLDA
                     ETLLKQLWQINDELGELLDSVENHTPVIRKKASDLSALMMLFSQQAGQPMTMLLIC"
     gene            843..1130
                     /locus_tag="ACHKJF_005576"
     CDS             843..1130
                     /locus_tag="ACHKJF_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952722.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRTGLDIRAIC"
BASE COUNT          313 a          294 c          305 g          221 t
ORIGIN      
        1 cgggatgtaa ggcgcaacgc cgtttcgcaa accgcgcttc acgtcgttgc ggcgggtccc
       61 tgcccgttgt tctgtccggt gccattcacc tgtttaagcc cctgtcgccg ggggctgata
      121 cagccacaac ctgccgcact gactgtccag ggtaaatgaa cagcccacaa aattttttac
      181 ccaaaacccg gtaaaaaatt ttgcgttctg cccaggacag gtgcgtcagg ccgtggcagt
      241 gatgccccat gcggcgaagg ttctcaaaca ggtgatggtg aataccggag ggaacaaaag
      301 gcaggtcccc gcaaaacgtc aaaaccgcgg ctcccgaaac tcaacagatg atgggggctg
      361 aaaacatcag atacaggagg aaacatgagc gcattttctt ttaacaccgc ttatcagcca
      421 acaggcgatc agcagaaaga cgcaatcgca caaatcgaca tcatgcagaa tcgtgctgtt
      481 caggcaaatc ttgcatacca gagcagcctg gatgcggaaa cgctattgaa acagttatgg
      541 caaatcaatg atgaactggg ggaactgttg gatagcgtgg aaaatcacac gccagttatc
      601 agaaaaaaag ccagtgattt gagcgcactg atgatgcttt tcagccaaca ggccggtcag
      661 cctatgacta tgttgttgat ttgttaatga taatcagggg caggaaatta agcgaaagat
      721 gctctgcccc gggtttgcag cgaccggaac ggtcacgcca cccttgtcgc gctgtggcct
      781 gcggcggctc ccgaaactca accgatgatg ggggctgaaa accagagaga ggagaaagca
      841 ggatgagtac ccgaaatatc cacgttaaca ccgcgtcgta cacgcttctt gttgccggga
      901 agaaaaaaaa caccggggaa gagtgggacg tcctggaatt cagcagcctg actgagctga
      961 aaaaatatcg caaaagccac ccggaaaaga tggccttcag ctatagctac gcgctcagcc
     1021 ggggtgtgga tacgcagttc cgccatatca acattgcgga agccgatcac ttcaaacagt
     1081 tcctgcgcca gattaagcgt accggcctcg atatccgggc gatctgctga ccg
//
LOCUS       DAZGXZ010000363         1114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000363 DAZGXZ010000000
VERSION     DAZGXZ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.366"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..207
                     /locus_tag="ACHKJF_005577"
     CDS             <1..207
                     /locus_tag="ACHKJF_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0952723.1"
                     /translation="EAGNKTENHTALWGCIRSRTQHGDKCTIPLLRDDMKKLGYDTKH
                     LKRWLAKLEKDDVIYIDGDDVGPL"
     gene            410..661
                     /locus_tag="ACHKJF_005578"
     CDS             410..661
                     /locus_tag="ACHKJF_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952724.1"
                     /translation="MTQKHRDRTEPKYKALDMTEHALKVAIRIIDRHAGEGYAKEHPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINIKTGEVES"
     gene            658..1080
                     /locus_tag="ACHKJF_005579"
     CDS             658..1080
                     /locus_tag="ACHKJF_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0952725.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMAMAGMAIPLPCSQADDGT
                     ATMWLSVLAFGRQADALAKHHKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT          368 a          251 c          320 g          175 t
ORIGIN      
        1 gaggcaggga acaagacgga aaaccatacc gcgctatggg ggtgcatacg ttcacgcaca
       61 cagcacggcg acaagtgcac gatcccgctg ttacgtgacg atatgaaaaa gctggggtat
      121 gacacaaaac accttaaacg atggttagcc aaactggaaa aagacgacgt gatctacatt
      181 gacggtgatg atgtaggacc actgtaaaaa gtgggcatta aaagtgggaa aggtgggcaa
      241 tttaacgtaa ttttaacaaa attacccact ttcccacgtg tatatatccc aaaaagtggg
      301 cactaaaaaa atacttatga aacaagatga tataaatccc aaatttccca cgttggacga
      361 agtaggaaaa cgtaaaaagt gggcgaaaaa agtgggtaga ggtgggcaaa tgactcaaaa
      421 acacagagac agaacagagc caaaatataa agcgttagac atgacagagc acgccttaaa
      481 ggtggcaatc aggataatag accgccacgc gggggaagga tacgcgaagg aacatcccga
      541 actgataagc gcattcatga ccacagcggc ggcaaacttt gccacgctga cagaacggga
      601 gattgccgaa gcggaacagg taacaaccat caacattaaa accggagagg tggaatcatg
      661 acagcacaga tagccgctta cgggcggctg gtggctgacc cacagttaaa gaccaccagc
      721 aagggtacac aaatggcgat ggcgggtatg gctatccccc tgccgtgcag ccaggccgat
      781 gacggaacgg cgacgatgtg gttatccgtc ctggcgtttg gcagacaggc cgacgcactg
      841 gcaaaacacc acaaaggcga actggtgagc gtggcgggta acatgcaggt gagccagtgg
      901 acaggccaga acggcgaaac gcggcagggg tggcaggtta tcgctgacag cgtaatcagt
      961 gccagaacgg cgcgaccagg tggtaaaaaa ggccagcagg gccaggctac tgacgcactg
     1021 aacagggcaa aacaacaggc gggtaacgat gatccgtacg gggataacat accgttttaa
     1081 gcaacgagtg acagaagccg gagcaatccg gctt
//
LOCUS       DAZGXZ010000364         1113 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000364 DAZGXZ010000000
VERSION     DAZGXZ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.367"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..520
                     /locus_tag="ACHKJF_005580"
     CDS             <1..520
                     /locus_tag="ACHKJF_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0952726.1"
                     /translation="QNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRD
                     PDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYV
                     GPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGI
                     FNYLKYYKEKCF"
     gene            546..>1113
                     /gene="rfaY"
                     /locus_tag="ACHKJF_005581"
     CDS             546..>1113
                     /gene="rfaY"
                     /locus_tag="ACHKJF_005581"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFP0952727.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKA"
BASE COUNT          396 a          160 c          201 g          356 t
ORIGIN      
        1 gcaaaatcga gcctcggtat taactacacc acagttagct tctggatatt tcaatgctgg
       61 ttttttactg ataaatattg atgagtggaa tctaaataac atttcgtcaa aagctattga
      121 aatgttgcgt gacccagatt gggtaagtaa aatcacccac cttgatcaag atgtactgaa
      181 tgtattattg aatggtaaag tgaagtttat ttcggagaaa tataataccc gatatagtat
      241 taactatgaa ttaaaagaca aagttgataa tccagtcaat gatgacaccg tgtttataca
      301 ctatgtggga cctacaaaac cctggcatga gtgggctgac tatccggtgt cacgtagttt
      361 tttgatcgcc aaagcagctt ctccgtggag taaagaagat ttacttaaac ctgtaaatag
      421 caatcagtat cggtattgtg caaaacataa atttaaacaa aagcattata tggcaggcat
      481 ttttaattat ttaaagtatt ataaagaaaa atgcttctaa agatttaagg ttaaaataat
      541 tcattatgat atataataaa acgataaatg ggttgaaagt tttcataaaa gataatgatc
      601 ccttttatga gcaagttcta aatgactttt taacttgcag ggttaaaaca ttaaaagttt
      661 ttcgttcgat tgatgatact aaagtcattc ttattgacac tgctcgtgga cctcttgtac
      721 ttaaagttta tgcgccaaag cataaaatga ccgaacgttt cttaaaatct tgcataaaaa
      781 aagattatta tgaaaattta atttatcaaa ccgatcgggt acgtggtgaa ggcatccaat
      841 caattaatga ctattttttg ctagctgaac gtaaaacatt aaatttcgct cattattata
      901 ttatgctcat tgagtatatt gaaggtgtcg ggttaaatga gtatttggaa atttctgaag
      961 atctgaagga tcaattatca gaatcgataa aagagttgca tcagcatgga atggtgtctg
     1021 gcgatcctca taaaggaaat tttatagtat ccgaaaaagg tttaagacta attgaccttt
     1081 caggtaaaaa aacaaccgct gttttaaaag caa
//
LOCUS       DAZGXZ010000365         1112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000365 DAZGXZ010000000
VERSION     DAZGXZ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.368"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..441)
                     /locus_tag="ACHKJF_005582"
     CDS             complement(<1..441)
                     /locus_tag="ACHKJF_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0952728.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFK"
     gene            699..>1112
                     /locus_tag="ACHKJF_005583"
     CDS             699..>1112
                     /locus_tag="ACHKJF_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0952729.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHLFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYHKIRALNKLN"
BASE COUNT          383 a          209 c          153 g          367 t
ORIGIN      
        1 cttaaacagc ttactgctaa agaaatgttg ttttgtagaa ataatattat tttcaataat
       61 gcctggcaat gaatactgcc aaacttcatt tttttctttt acagcaaatt tgagacctaa
      121 aacttctatt tgctgattca atgttaacgt tctatcttca ggttcttgtg ctgttaattc
      181 tctgaatgtt actaccagat cagggtcggt agtaattatt atctcaaaac tacctgtaag
      241 ctcgccataa ggaacgacta ttgtgttttc tggtaactca gaaaaagcat cacttcttcc
      301 taacgaaaca tcattaccaa tgataacaag tagacgccca ttaagtattt catactcgca
      361 tccattcaat ggtccgctca acaaacgaat ggcataagat tccaataagt cttgtgataa
      421 gaatttatca ttattttcca tgaataccac ccgctaattc cataagctct agtttagagt
      481 agtgtaacaa gacggaaatt tcatgaccaa attatgatga cacagaataa aaaaattata
      541 attccgagaa ctattttaaa tgcctctatg cataacttta tttacaattt aaggtataaa
      601 ccctattccc tcaatgttaa gcaaatataa caattactct tattttaaat tttacacgta
      661 atatttaccc taaatgattt tagagtacag gtgggcatat gcaagtcttt tccagtgatg
      721 tctacttcac tgtaggcact aatgcattat tagcatccca aaaagaatat tacagcgatt
      781 tagttgcgtt ggttgatctt ggacatttgt tcgttgttat tgatgagcat caacatcgaa
      841 atctcaaacc aaatacagaa cctgtaaaca tccttttgag caacaatttc atcaggatca
      901 ataagaatat tacattatct gatttaacgc atttcctggt ttcaaatctt catacacaaa
      961 atgtgtattc cacacaagaa ccacttaccc atgatgagat agatatcctg cggttatgtg
     1021 tttcttactc gttaaaacaa atagccatta ttaaaggaat tgattataaa acaatatcat
     1081 atcacaaaat aagagcccta aataaactta ac
//
LOCUS       DAZGXZ010000366         1110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000366 DAZGXZ010000000
VERSION     DAZGXZ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.369"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(844..1098)
                     /locus_tag="ACHKJF_005584"
     CDS             complement(844..1098)
                     /locus_tag="ACHKJF_005584"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0952730.1"
                     /translation="MSESLLKRKLQEEKVSFSQILLDIRMHNARQLISSKLSVNQIAI
                     RCGYASTPYFISVFREYFGITPHQMMINQSGFIESRIAIK"
BASE COUNT          351 a          218 c          208 g          333 t
ORIGIN      
        1 tgagtgctga atctatttta aatggtatca taaatacctg tccacactta agattttcct
       61 gcggaaaaca aatattaaca tattttatgt attccgcatg gtgatgattt atacacctgc
      121 actgaatttt tctgtatctc cagagctgaa tcgataaaca gaagtggtaa taatgaaagt
      181 taatcacata aaaaagaggt taccgcctta taatgataag agttcattat ccattccatc
      241 cagacaaaac ctgcctgtgt ataaaaaata agaaccatgt cacaaaaaca ttgcattccg
      301 gcaagttatg acagaattac tgaaaaccgt tatctgtagt tattgtagtt tctgatatat
      361 gctcatccac ccggaaatac gacatggtcc tgactggcgt tcagttcaaa gtgtaattta
      421 ttattcacac actgagactg aaaaacggat ggcgtggatg agtacacagc ctgaagagta
      481 taaaaggaac agctccggat tgttacccgg gattactata gttcaccaca caaccgtaaa
      541 cctggttgtg tggtgaaaca gtaacatatt aagaatattg tgttaccacc tgttttctca
      601 ggaaacggat tgcaggagag gtatactcat tcatcttccc ggagttttgt taatcactgc
      661 ataatcaaac ggaccaatac attctttgct cgcatccgta aaaatgtcgc cacaatatca
      721 tcagtcagtc aactcattac catctgtttt ccggggccgt agttactaca gtgctccgga
      781 gtgataaaca gaaactgata atgaagcata agaatacttt tattatgcgc tgactgacat
      841 actttactta attgcgattc tgctctcaat aaatccggac tgattaatca tcatctgatg
      901 aggtgttatg ccaaaatatt ctctgaaaac gctgataaaa tacggtgtgc tggcataacc
      961 gcaccgtatt gcgatttgat tcactgacag tttactggaa ataagttgtc gggcgttatg
     1021 catgcgtata tccagcagga tttgggagaa agaaactttt tcctcctgaa gttttctttt
     1081 cagtaaactc tcgctcatgt acagagaatt
//
LOCUS       DAZGXZ010000367         1109 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000367 DAZGXZ010000000
VERSION     DAZGXZ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.370"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            211..>1109
                     /gene="tssI"
                     /locus_tag="ACHKJF_005585"
     CDS             211..>1109
                     /gene="tssI"
                     /locus_tag="ACHKJF_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP0952731.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETSRGMSRSPEIWPGR"
BASE COUNT          250 a          302 c          295 g          262 t
ORIGIN      
        1 cgagaaccca tccgttgcgt ttgtgattat tttgttgact aaacagacac ccgtttctct
       61 gaagtaaaat cccagactaa atcatcacat aaccatgaca tttttctgat attccccggt
      121 aacgccagat gtcgactcgc ttatctcatc cgcccgacat gttgtacatg caatatgtgc
      181 ggattattat tcattttcac ggaggttgct atgtcaaccg gattacgttt cacacttgaa
      241 gtggacggcc tgccaccgga cgcgtttgcg gtggtttcct ttcatctcac ccagtcactc
      301 tcttcacttt tttccctcga cctttctctg gtcagtcagc agtttctttc ccttgaattt
      361 gcgcaggtgc tggacaaaat ggcctacctg acggtatggc agggcgatga cgtacagcgc
      421 cgggtgaaag gtgtggtgac ctggtttgaa ctgggggaga acgacaaaaa ccagatgctg
      481 tacagcatga aggtgtgccc gccgctgtgg cgcacagggc tgcgccagaa cttccgtatc
      541 ttccagaatg aggacatcga aagcatcctc gctacgatcc tgaaggaaaa cggtgtgacc
      601 gagtggagtc cgctgttcag cgagccgcat ccttcccgtg agttttgtgt ccagtacggc
      661 gaaaccgatt acgatttcct gtgccggatg gcggcggagg aaggcatctt cttttatgag
      721 gagcacgcgc aaaaaagtac cgaccagagc ctggtcctgt gcgacaccgt gcgttatctg
      781 ccggagtcct ttgagatccc ctggaatccg aacacccgta ccgaggtgag caccctctgc
      841 atcagccagt tccgctacag cgcacaaatc cgcccttctt ccgtggtgac caaggactac
      901 acctttaaac gacccggctg ggcagggcgt tttgatcagg aaggccagta ccaggattac
      961 cagcgcacgc agtatgaagt gtatgactac cccggacgtt tcaagggtgc ccacgggcag
     1021 aactttgccc gctggcagat ggacggctgg cgaaacaatg cagaaacctc gcggggaatg
     1081 agtcgctcgc cggagatatg gccgggacg
//
LOCUS       DAZGXZ010000368         1108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000368 DAZGXZ010000000
VERSION     DAZGXZ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.371"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(122..661)
                     /locus_tag="ACHKJF_005586"
     CDS             complement(122..661)
                     /locus_tag="ACHKJF_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0952732.1"
                     /translation="MSNIGYKRVSTVDQNTDRQLAGVTLDKVFEDKLSGKDTNRPGLQ
                     ACLSYLREGDTLHVHSLDRLGRNTLDILALVEQLNNRGVIVRFHKEGIIADITSAMGK
                     MMLTMLSAVATCERELMLERKREAQAVNPTKRGNGKAVDREGIKKALSAGVSVRKTAE
                     IFNVAPSTVQRIKKEEEEA"
BASE COUNT          318 a          239 c          212 g          339 t
ORIGIN      
        1 ctataaatac atctaaatac ctatattcct tcccttatgg ctagattgcg agaggtgctt
       61 aacgcgattt tcagcgcccc ccgatctgga aaatccagaa tccccaaata tcaaggaggc
      121 attacgcctc ctcttcttcc ttcttgatac gttgcaccgt tgaaggagca acattaaaga
      181 tctcagcggt tttacgcact gacacgccag cggaaagggc tttctttata ccttctcgat
      241 ccactgcttt tccattacca cgttttgtag gattgacggc ttgcgcttct cgtttacgtt
      301 ctaacatcag ttcacgttca cacgttgcca ctgccgacaa catcgtaagc atcatctttc
      361 ccatcgcgct tgtaatgtct gcaatgattc cttctttgtg aaatcgaaca atcacacctc
      421 gattattaag ttgttcaact aaagcaagga tgtctaaggt gttacgtcct aaacggtcga
      481 gagagtgcac gtgtaaggtg tcgccttctc tcagatagga taaacacgcc tgcaatcctg
      541 gacggttagt gtctttacct gacagcttat cttcaaagac tttatcaagc gttactcctg
      601 caagctggcg atcagtgttc tgatctacag tgctcacacg cttataaccg atattgctca
      661 taaaatcctc ttaatgtatc gatttggcac tacttcaatg caaatgagta tatcgtattc
      721 atttggtctg tttcaagcct tataggtacg gaatattaca cgatttagct gtattcattt
      781 ggtatgccta atggatacac agggagatag attaaatggt gttctgttta tacaggttta
      841 tttgttagat caacaatttg gcatttctaa acagatgttt gaacgttgcc aaatgttttg
      901 acaacacaag aaaagggctt gctatgccac gcattacacg aaataaggct gtaaaaacaa
      961 aataaatttt aaaatttgca caaacgagaa actcgttcga taaataatag attgcttttt
     1021 agccaacaat ttctgatggt gtgctgtaag ccgcattaca caaggctttg cgaaagtgat
     1081 gtaaaagtga agaaagtaag aattgaac
//
LOCUS       DAZGXZ010000369         1108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000369 DAZGXZ010000000
VERSION     DAZGXZ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.372"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1108
                     /locus_tag="ACHKJF_005587"
     rRNA            <1..>1108
                     /locus_tag="ACHKJF_005587"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          300 a          233 c          355 g          220 t
ORIGIN      
        1 tgaatatggg gggaccatcc tccaaggcta aatactcctg actgaccgat agtgaaccag
       61 taccgtgagg gaaaggcgaa aagaaccccg gcgaggggag tgaaaaagaa cctgaaaccg
      121 tgtacgtaca agcagtggga gcacagagca atctgtgtga ctgcgtacct tttgtataat
      181 gggtcagcga cttatattct gtagcaaggt taaccgaata ggggagccga agggaaaccg
      241 agtcttaact gggcgttaag ttgcagggta tagacccgaa acccggtgat ctagccatgg
      301 gcaggttgaa ggttgggtaa cactaactgg aggaccgaac cgactaatgt tgaaaaatta
      361 gcggatgact tgtggctggg ggtgaaaggc caatcaaacc gggagatagc tggttctccc
      421 cgaaagctat ttaggtagcg cctcgtgaat tcatctccgg gggtagagca ctgtttcggc
      481 aagggggtca tcccgactta ccaacccgat gcaaactgcg aataccggag aatgttatca
      541 cgggagacac acggcgggtg ctaacgtccg tcgtgaagag ggaaacaacc cagaccgcca
      601 gctaaggtcc caaagtcatg gttaagtggg aaacgatgtg ggaaggccca gacagccagg
      661 atgttggctt agaagcagcc atcatttaaa gaaagcgtaa tagctcactg gtcgagtcgg
      721 cctgcgcgga agatgtaacg gggctaaacc atgcaccgaa gctgcggcag cgacgcttat
      781 gcgttgttgg gtaggggagc gttctgtaag cctgtgaagg tgtactgtga ggtatgctgg
      841 aggtatcaga agtgcgaatg ctgacataag taacgataaa gcgggtgaaa agcccgctcg
      901 ccggaagacc aagggttcct gtccaacgtt aatcggggca gggtgagtcg acccctaagg
      961 cgaggccgaa aggcgtagtc gatgggaaac aggttaatat tcctgtactt ggtgttactg
     1021 cgaagggggg acggagaagg ctatgttggc cgggcgacgg ttgtcccggt ttaagcgtgt
     1081 aggctggttt tccaggcaaa tccggaaa
//
LOCUS       DAZGXZ010000370         1101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000370 DAZGXZ010000000
VERSION     DAZGXZ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.373"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..200
                     /locus_tag="ACHKJF_005588"
     CDS             <1..200
                     /locus_tag="ACHKJF_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0952733.1"
                     /translation="KGVAGRCVTYRREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSN
                     ARARANIQKLKTMINGFRGKK"
     gene            197..>1101
                     /locus_tag="ACHKJF_005589"
     CDS             197..>1101
                     /locus_tag="ACHKJF_005589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0952734.1"
                     /translation="MKLAPNVKRLPKDKYTDAIIFAGIDAHSFAEHYIIAQAKKAGDP
                     VPPVYLGRYQLSELDNLQIVDDGRYRATVIRAGNIEEPQLLTIATKLASAGVQEARLL
                     SENFELLEEWSDQLPRLREAWERGESLVMKKIPQRKTKLPMSVGSTGYDTQLDYVVKG
                     IIPASSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPR
                     RVKAWEVVHDEQVKNLYLVNRPIFPAAPLDIDEMVIAASQVERETGKPVRMIILDTLA
                     RCFGGNDENDSRDMGAFIRGCDELKR"
BASE COUNT          273 a          256 c          325 g          247 t
ORIGIN      
        1 ttaagggcgt ggcgggacgg tgcgtaactt accgccgtga aggttacgaa tatgattgcg
       61 tgatgcctgt ttatcagttc cggcgtgatt tttctctggt acagaccgcg ccgcataacg
      121 tgcccaccag caacgccagg gcacgggcaa acatccagaa gctgaaaacc atgattaacg
      181 gattcagggg taaaaaatga aactggcacc gaacgtaaaa cgattaccga aagataaata
      241 caccgatgcg attatttttg cgggtattga tgctcattca ttcgcagagc attacatcat
      301 tgcacaggcc aaaaaagcag gcgatccagt ccctcccgtt tatctggggc gttatcagtt
      361 aagcgaactg gataacctcc agattgttga tgatgggcga tacagggcga cggtgatacg
      421 cgccggaaat attgaagagc cgcagctgtt aaccatcgcc acgaaactgg cgagcgccgg
      481 agtccaggag gcgcggctac tttctgaaaa tttcgaattg ctggaggaat ggagcgacca
      541 gcttccacgg cttagggagg catgggaacg cggggaaagc ctggtcatga aaaaaatccc
      601 ccagcgaaaa accaagctac caatgagcgt tggatctacc ggatacgaca cacagcttga
      661 ttacgtggta aaggggatta ttccggcatc gtcgctttgc agcatatacg gggcgagcgg
      721 ttcctataaa tcattccttg ccggatcgtg ggcgtgtcac gtttccacgg gccgccagtg
      781 gggaggccgc agggtggcgc atggcgctgt tctctatgtg gttggtgaag gcggtatagg
      841 cgttccgcgt cgtgtaaaag cctgggaggt tgtgcacgat gagcaggtga aaaatctgta
      901 tctggtaaac cgtcccatct ttccggctgc cccgcttgat attgatgaaa tggttatcgc
      961 tgccagccag gtggagcggg aaacgggtaa acctgttcgc atgattattc tggacacgct
     1021 ggcgcgttgc tttggcggca atgatgaaaa tgactcccgc gacatggggg catttatccg
     1081 tggttgtgac gagcttaaac g
//
LOCUS       DAZGXZ010000371         1084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000371 DAZGXZ010000000
VERSION     DAZGXZ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.374"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..339
                     /locus_tag="ACHKJF_005590"
     CDS             46..339
                     /locus_tag="ACHKJF_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP0952735.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(400..1084)
                     /locus_tag="ACHKJF_005591"
                     /pseudo
     CDS             complement(400..1084)
                     /locus_tag="ACHKJF_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          351 a          203 c          173 g          357 t
ORIGIN      
        1 ataaacatta ttttaaactc caatcttgcg taagagaaag ttgatatgtc attagtagga
       61 tataatagta acatcataat aaccatcgcg ctgatatcgt tgctctctgc atgcaccaga
      121 gaaaattcat taaaggagaa tcaaaataaa agaagtggcg aatcccaagc gatagtagat
      181 gcaagacagt tctttagcga acacccggaa tatatatcat cagcagaact ggagcaaaag
      241 ctatatcgcg aatttgcaca ggttactaca gttccagctt ataaagagat gagtatgtat
      301 caattgctta tcatttcaca agatcgttta actcattaaa taaggatcta cacgcttttt
      361 gcgtgtagat ccttataaat ttctgactac gacagatgtt taatttttta aattatcgtc
      421 tttattctcg atttttgtgt cttcaccctc aatgaattta tcttctgaag agagctgttc
      481 atcttgaaca ggttgcccag cattctccac atcttcaagc cacagcagaa gacgtaatat
      541 ttcatctata ttttcgaagc taacataatc gtagcgacga tgggtagcat aaatttttcg
      601 tgctaatttt ttatctgtga taattggtat tccgatttcc tttgcatatt tccttacgcc
      661 taaggccacc tcattagtta ctcttacaga aatcaatgga ataggtgaaa gatgtggctt
      721 aaagtatatc cctattgcaa tgtgagtagg gttggaaatc atcaaacgac tattactgac
      781 atcagacttc aattgctcag aaagaatttc ctgatgcgct ctctgcgttt agacttaatt
      841 tctggatttc cttcttgctc cttgtattct cttttaactt cttgtttatc cattttcata
      901 tctttcataa ataaaaaata ttcagcgata aaatcaaaaa ttaagacaat tatcatactg
      961 ccgagacaat acagtatgag aaggaatagc atctctcccc aatctgataa taaagatatg
     1021 atatctccat caagagtttt aaaaagcagt gatttatgat tactccaaaa tactttgatc
     1081 gcca
//
LOCUS       DAZGXZ010000372         1077 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000372 DAZGXZ010000000
VERSION     DAZGXZ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.375"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..298
                     /locus_tag="ACHKJF_005592"
     CDS             <1..298
                     /locus_tag="ACHKJF_005592"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0952736.1"
                     /translation="VTSLYEGGPANIPEAIASGTPIISTPVGMVNDYVRDGINGLILT
                     GDIDNDIDSINNFSQKSKYQKLSSNALEMSGNIMSWQDVMQKTSLVYKELAGCA"
     gene            299..>1077
                     /locus_tag="ACHKJF_005593"
     CDS             299..>1077
                     /locus_tag="ACHKJF_005593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFP0952737.1"
                     /translation="MKNISILFADSIHFHVRNFKSLFDLIDRHKIHHTFIHERNKWVS
                     AYGNYEEFFNELQPLYNKLLGKDIEQLYSLTIYGINIFKVCRDELLSYYLPIDSFRRL
                     LTGVPDDRNILLYMMQIDQRTLLLNFAAAWSWLEFWKEKLSKIRNHTYACIFSGAQIY
                     NRTLLELLKTHPTIPLVLEHFFTGNEYYMEEKYDPIPNNTNLKINNTYNSIIIEEHGF
                     ELDRERIKAINKVLLSKNKNVRQPSDTLLLEKKNSKTVTIIG"
BASE COUNT          390 a          155 c          199 g          333 t
ORIGIN      
        1 agtaacgagt ttgtacgaag gtggaccagc aaatatacct gaagcaattg catccggtac
       61 gccaataata tcaactcctg tgggtatggt aaatgattat gtcagggatg gtattaatgg
      121 tcttatatta actggagata ttgataatga tatcgactct ataaacaatt tctctcaaaa
      181 aagtaaatat caaaaattat caagtaatgc tcttgaaatg tctgggaaca taatgtcttg
      241 gcaggacgtt atgcaaaaaa catcattggt atacaaagag ttagcagggt gcgcttgaat
      301 gaaaaatata agtatattat ttgcggacag tatacatttt catgtgcgta attttaaaag
      361 tctattcgat ttaatcgaca ggcataaaat tcaccatacg ttcatacatg aacgaaataa
      421 atgggtgagt gcttatggta attatgagga attctttaat gaattacagc cattatataa
      481 taaattgctg gggaaagata ttgaacaatt gtattcactt acaatttatg gaattaacat
      541 ctttaaagta tgccgggatg aattattatc gtattatttg ccaattgata gctttagacg
      601 gttgttaacc ggtgtgccag atgatagaaa tatattattg tacatgatgc aaatagatca
      661 aaggacattg ttattaaact ttgcggcagc atggtcctgg cttgagtttt ggaaagaaaa
      721 acttagtaaa ataagaaatc atacttatgc gtgtattttt tctggtgccc agatatataa
      781 caggacccta ttagaacttc tgaaaacaca tccgacaatc cccctggtac tagaacattt
      841 ttttactggc aatgaatact atatggagga aaaatacgat cctattccta ataacacaaa
      901 tttaaaaata aataacacat ataattcaat tataattgag gagcatggtt ttgaacttga
      961 ccgggaaagg ataaaggcaa ttaataaggt tcttttgtct aagaataaaa atgttcggca
     1021 accctctgac acattgctac tcgagaaaaa aaacagtaaa acagttacta ttattgg
//
LOCUS       DAZGXZ010000373         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000373 DAZGXZ010000000
VERSION     DAZGXZ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.376"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1076
                     /gene="ygeH"
                     /locus_tag="ACHKJF_005594"
     CDS             <1..>1076
                     /gene="ygeH"
                     /locus_tag="ACHKJF_005594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP0952738.1"
                     /translation="LKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIY
                     RKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGL
                     YTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIAS
                     NHLPVDELHNTSQFIIENILQTVHNPQRSVRLTKQDQGYKNHYLSDEMLAGKKELYEF
                     TPESIYRAMTIFDGLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELL
                     DYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVNFHN
                     EKIEEARICIDKSLQLEPRRRKAV"
BASE COUNT          387 a          195 c          179 g          315 t
ORIGIN      
        1 actgaaagat atgatcatcg aatcagtatg gaaaaatatt attgttagtg acgagtccct
       61 gacaagatgt atctattctt tgcgctgcat ttttgaaaaa attggctatg atcgttgcat
      121 agaaacaatc taccggaaag gttatcgttt cagcgggcag gttttcaaaa ctaaaattaa
      181 tgaagataat acttcagact attccatagc tatattccct ttcactactt cattgaatac
      241 actggatcca ttaatactta atcaggaatt agtgcaaatc atttcaaata aaaaaatcga
      301 tggtctctat acctatccga tggctgcgac aaatttttgt aatgatcaca tatctcaaaa
      361 ttcattcttg agcagattca agccagatta tttcgttaca ggaagaataa accagaataa
      421 tgcagtgaac actttataca ttgagttgat cgacgctaaa aaccttttcc tcatcgccag
      481 taatcatcta cctgttgatg aactacataa tacatcacaa tttattatag aaaatatcct
      541 tcaaacagta cataatccac aacgatctgt aagattaact aagcaggacc aaggatataa
      601 gaatcattat ttatcagatg aaatgttagc cggaaagaaa gaactttacg agttcacccc
      661 tgaaagcatt tacagggcca tgactatatt tgatggatta caaaataaaa gtgatataca
      721 gacgctaaaa acagaatgtt attgccttct agcggaatgc catatgtctt tggcacttca
      781 tggaaaaagt gaacttgaac ttgctgctca aaaagcatta gagcttttag attatgtatc
      841 agacataacc actgtcgatg gaaaaatttt agctattatg ggactgataa ctggtctgtc
      901 tggacaagca aaagtatctc atatcttatt tgaacaggct aagatacact caactgatat
      961 agcctctctc tactactata gggcacttgt caactttcat aatgaaaaaa ttgaagaggc
     1021 aaggatttgt atagacaaat cactacaact cgaacccaga agacgaaaag cagttg
//
LOCUS       DAZGXZ010000374         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000374 DAZGXZ010000000
VERSION     DAZGXZ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.377"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..468
                     /locus_tag="ACHKJF_005595"
     CDS             46..468
                     /locus_tag="ACHKJF_005595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP0952739.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKADTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
BASE COUNT          328 a          247 c          242 g          259 t
ORIGIN      
        1 atataactcc ctgagtatca agattaaaaa cgcaaggaga tgtttatgag atctgccgct
       61 gccaggctgc ttctgatacc tctgataaca gcaacaatag ctcttacagg atgcacacca
      121 aagaccagcc tggaacgaca tacccggcat tatgtttatg cttcagatga tggatttgat
      181 cctaacttct acacccagaa agcagacacc atacgtatga tgctcccgtt ctttcagcag
      241 ttccgggata tggggatgaa agacaaagct gccggagtat cagcagaaac ggcacagcaa
      301 cgtgtaaaag aattccactc agaaaaattt tttcactcac tccggagcac aacaaccttt
      361 gctggcagaa aatacacaaa cagcgatatg ccttcgccga aaaaaatgaa actaatggca
      421 gacaccattt ctgcggttta tctcgatgga tacgagggca gacagtaagg gatttaccat
      481 aatcccttaa ttgtacgcac cgctgaaacg cgctcagcgc gattctggcg caaaaaccgc
      541 aaaaagagat gttttccccc gggtaaaccg gaaaaatacg tcaggattgt tttcatggca
      601 ctgcatgact atgcatgaaa ctgaatggcg atcggtttgg gcgcgcctga tgcccataag
      661 gcgcattttc ggacgttttc agccctgatg agaagaaatc agacagtagt tacagacgag
      721 tcgtgagcga ttcaccccgg gagtcatcag cgagtcatcc agtatttttc ctcgcgactc
      781 tctggcgact cactttttct gaacaccaga gagacagtgt gttgagtcat cgataaatca
      841 ccgacgactc gttgccgaat catccagtag tcgccgacaa ctcgcttttg tataaatctg
      901 aataagaaaa tatatttttc aaaccataac aacatgattt aaaaagcaaa tcagaaaaaa
      961 gttggttttg cgtggggggt gggcatccgg ggcatgagaa cagactcgcg tttttctgga
     1021 gaaactgcgg ggatttttga ttaaacaata ctcatcgcag agcggaattt tatgca
//
LOCUS       DAZGXZ010000375         1076 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000375 DAZGXZ010000000
VERSION     DAZGXZ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.378"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            46..468
                     /locus_tag="ACHKJF_005596"
     CDS             46..468
                     /locus_tag="ACHKJF_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP0952740.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKSDTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            complement(838..>1076)
                     /locus_tag="ACHKJF_005597"
     CDS             complement(838..>1076)
                     /locus_tag="ACHKJF_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952741.1"
                     /translation="HKIPLCGEYCLIKNPRSFSRKTRVCSHAQDAHTLRKTNFLLISF
                     LNQVVMFKKMYFLIQMYTKASRRRLLDDSATSRR"
BASE COUNT          324 a          249 c          241 g          262 t
ORIGIN      
        1 atataactcc ctgagtatca agattaaaaa cgcaaggaga tgtttatgag atctgccgct
       61 gccaggctgc ttctgatacc tctgataaca gcaacaatag ctcttacagg atgcacacca
      121 aagaccagcc tggaacgaca tacccggcat tatgtttatg cttcagatga tggatttgat
      181 cctaacttct acacccagaa atcagacacc atacgtatga tgctcccttt ctttcagcag
      241 ttccgggata tggggatgaa agacaaagca gccggagtat cagcagaaac ggcacagcaa
      301 cgtgtaaaag aattccactc agaaaaattt tttcactcac tccggagcac aacaaccttt
      361 gctggcagaa aatacacaaa cagcgatatg ccttcgccga aaaaaatgaa actaatggca
      421 gacaccattt ctgcggttta tctcgatgga tacgagggca gacagtaagg gatttaccat
      481 aatcccttaa ttgtacgcac cgttaaaacg cgctcagcgc gtttctggcg caaaaaccgt
      541 aaaaagggat gttttccccg gggtaaaccg gaaaaatgcg tcaggaacgt tttcagcgcg
      601 ttgcatgact atgcatgaaa ctgaatggcg atcggtttgg gcgcgcctga tgcccataag
      661 gcgcattttc ggacgttttc agccctgata agaagaaatc agacagtagt tacagacgaa
      721 tcgtgagcga ttcaccccgg gagtcgtcgg cgagtcatcc agtatttttc ctcgcgactc
      781 tctggcgact cgccttctct gaacaccaga gagacagtgt gttgagtcat cgataaatca
      841 ccgacgactc gttgccgaat catccagtag tcgccgacga ctcgcttttg tatacatctg
      901 aataagaaaa tacatttttt taaacataac aacctgattt aaaaaagaaa tcagcaaaaa
      961 gttggttttg cgtagtgtgt gggcatcctg ggcatgagaa cagactcgcg tttttctgga
     1021 gaaactgcgg ggatttttga ttaaacaata ctcaccgcag agcggaattt tatgca
//
LOCUS       DAZGXZ010000376         1073 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000376 DAZGXZ010000000
VERSION     DAZGXZ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.379"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>1073
                     /gene="rhsB"
                     /locus_tag="ACHKJF_005598"
     CDS             <1..>1073
                     /gene="rhsB"
                     /locus_tag="ACHKJF_005598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFP0952742.1"
                     /translation="CATDDATGSRKTMTWSRYGQLLSFTDCSGYVTRYGHDRFGQMTA
                     VHREEGLSQYRAYDSRGQLIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAW
                     GKAICTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYH
                     HDLTGKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAERWQYDERGWLTDISHISEGH
                     RVAVYYGYDEKGRLTGERQTVHHPETEALLWQHETRHAYNAQGLANRCIPDSLPAVEW
                     LAYGSGYLAGMKLGDTPLVDFTRDRLHRETLRSFGRYELTTAYTPAGQLQSQHLNSLL
                     SDRDYTWNDNGELIRISSPRQTR"
BASE COUNT          253 a          333 c          327 g          160 t
ORIGIN      
        1 ctgcgcaacg gacgatgcca ccggcagccg gaaaaccatg acgtggagcc gttacggcca
       61 gttgctgagc ttcaccgact gttccggtta tgtaacccgt tatggccatg accgcttcgg
      121 gcagatgacg gcggtgcacc gcgaggaagg gctgagccag taccgtgcat acgacagccg
      181 tggacagtta attgccgtga aagacacgca gggccatgaa acgcggtatg aatataacgc
      241 cgccggtgac ctgaccaccg tcattgcccc ggacggcagc agaaacggga cacagtacga
      301 tgcgtgggga aaggccatct gtaccacgca gggcgggctg acgcgcagta tggaatacga
      361 tgctgccgga cgggtcatcc gcctgaccag tgaaaacggc agccacacca ccttccgtta
      421 cgatgtactc gaccggctga tacaggaaac cggctttgac ggccgcacac agcgttatca
      481 ccacgacctg accggcaaac ttatccgcag cgaggatgag gggctggtca cccactggca
      541 ctatgacgaa gcagaccgcc tcacgcaccg caccgtgaat ggcgaaaccg cagagcggtg
      601 gcagtatgac gaacgcggct ggctgacaga catcagccat atcagcgaag ggcaccgggt
      661 ggcggtgtat tatggatatg atgagaaagg ccgcctgacc ggtgagcgcc agacggtgca
      721 tcacccggag acggaagcac tgctctggca gcatgagacc agacatgcgt acaacgcgca
      781 gggactggcg aaccgctgta taccggacag cctgcccgcc gtggaatggc tggcctacgg
      841 cagcggttac ctggcaggca tgaagctcgg cgacacaccg ctggtggatt tcacccgcga
      901 ccgcctgcac cgggaaacgc tgcgcagctt cggccgttat gaactcacca ccgcttacac
      961 ccctgccggg cagttacaga gccagcacct gaacagcctg ctgtctgacc gcgattacac
     1021 ctggaacgac aacggcgaac tcatccgcat cagcagcccg cgccagaccc gga
//
LOCUS       DAZGXZ010000377         1052 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000377 DAZGXZ010000000
VERSION     DAZGXZ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.380"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..163
                     /locus_tag="ACHKJF_005599"
     CDS             <1..163
                     /locus_tag="ACHKJF_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0952743.1"
                     /translation="EGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKT
                     MINGFRGKK"
     gene            160..>1052
                     /locus_tag="ACHKJF_005600"
     CDS             160..>1052
                     /locus_tag="ACHKJF_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0952744.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPIVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGIHSQKRENWREYLERERGKKPGKEHHQRKTTLPMSVGSAGYDTQLDYVVKGIIPA
                     SSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVKA
                     WEVVHGEQVKNLYLVNRPIFPAVPLDVDEMVIAASQVERETGKPVRMIILDTLARCFG
                     GNDENDSRDMGAFVRGCDELKR"
BASE COUNT          265 a          236 c          325 g          226 t
ORIGIN      
        1 tgaaggttac gaatatgatt gcgtgatgcc tgtttatcag ttccggcgtg atttttctct
       61 ggtacagacc gcgccgcata acgtgcccac cagcaacgcc agggcacggg caaacatcca
      121 gaagctgaaa accatgatta acggattcag gggcaagaaa tgaaaagcgc accgaactta
      181 aaaaaacagc cttacgacaa gatgaccgaa gtcatcattt ttgcgggtag tgatgcgtgg
      241 gcacatgcga aacagtggca ggaacaggac gggcgactgg ctggcgataa tgtgcctccc
      301 attgtgctgg ctgatgatca actggatgaa ctggcagacc tgagaatcat cgacgagggg
      361 cgctattgtg tccggctgta caaggcaggc cacatcaggc catcaaatat taatgccatt
      421 gcgcacaagc tggcggcggc gggcgtaact gatgcgaatt attaccccga agggatacac
      481 agccagaagc gggagaactg gcgcgaatat ctggaacggg agcgggggaa aaagccaggg
      541 aaagaacacc accagcgaaa aaccacgcta ccgatgagcg ttggatctgc cggatacgac
      601 acgcaattag attacgtggt aaaggggatt attccggcat cgtcgctatg cagcatatac
      661 ggggcaagcg gttcctataa atcattcctt gcgggttcgt gggcgtgtca cgtttccacg
      721 ggccgccagt ggggaggccg cagggtggcg catggcgctg ttctctatgt ggttggtgaa
      781 ggcggtatcg gcgttccgcg tcgtgtaaaa gcctgggagg ttgtgcacgg tgagcaggtg
      841 aaaaatctgt atctggtaaa ccgtcccatc tttccggctg tcccgcttga tgttgatgaa
      901 atggttattg cagccagcca ggtggaacgg gaaacgggta aaccggtccg aatgattatt
      961 cttgatactc tggcgcgttg ctttggtggg aatgatgaaa atgattcccg tgatatgggg
     1021 gcgtttgtcc gtggttgtga cgaactgaaa cg
//
LOCUS       DAZGXZ010000378         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000378 DAZGXZ010000000
VERSION     DAZGXZ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.381"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..1046
                     /locus_tag="ACHKJF_005601"
     rRNA            <1..1046
                     /locus_tag="ACHKJF_005601"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          258 a          245 c          329 g          214 t
ORIGIN      
        1 agaagcaccg gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat
       61 cggaattact gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg
      121 ggctcaacct gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga
      181 attccaggtg tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc
      241 cccctggacg aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac
      301 cctggtagtc cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt
      361 ccggagctaa cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa
      421 tgaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa
      481 gaaccttacc tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga
      541 accgtgagac aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt
      601 cccgcaacga gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga
      661 gactgccagt gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg
      721 accagggcta cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa
      781 gcggacctca taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt
      841 cggaatcgct agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac
      901 acaccgcccg tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg
      961 agggcgctta ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg
     1021 ggaacctgcg gttggatcac ctcctt
//
LOCUS       DAZGXZ010000379         1046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000379 DAZGXZ010000000
VERSION     DAZGXZ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.382"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..119
                     /locus_tag="ACHKJF_005602"
     CDS             <1..119
                     /locus_tag="ACHKJF_005602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0952745.1"
                     /translation="PLAAKVLKQVMVNTGGNKEQVPAKRQNRGSRNSTDDGG"
     gene            143..664
                     /locus_tag="ACHKJF_005603"
     CDS             143..664
                     /locus_tag="ACHKJF_005603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0952746.1"
                     /translation="MAVRGINKVILVGRLGKDPDVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPEPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQQP
                     SEPAYDFDDDIPF"
     gene            722..955
                     /locus_tag="ACHKJF_005604"
     CDS             722..955
                     /locus_tag="ACHKJF_005604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFP0952747.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDCAGYWMNRYIRDFGILK"
BASE COUNT          276 a          260 c          325 g          185 t
ORIGIN      
        1 tgccccttgc ggcgaaggtt ctcaaacagg tgatggtgaa taccggaggg aacaaagagc
       61 aggtccccgc aaaacgtcaa aaccgcggct cccgaaactc aacagatgat gggggctgaa
      121 aacatcaaca gaaggagaca tcatggcagt tcgtggcatt aacaaggtca ttctcgtggg
      181 acgtctggga aaagatccgg atgtccgtta catccccaac gggggcgcag tggcaaatct
      241 gcaggtggcc acgtcagaaa gctggcgtga caaacagacg ggggagatgc gggagcagac
      301 agaatggcac cgcgtggtgc tgttcggcaa gctcgcggaa gtggcaggtg aatatctgcg
      361 taagggcgcg caggtctaca tcgaggggca actccgtacc cgtagctggg aagataacgg
      421 tattacccgt tacgtcactg aaatccttgt gaaaaccacg ggcaccatgc agatgctggg
      481 gagtgcacca cagcagaacg ctcaggcgca accggagcct cagcagaatg ggcagccaca
      541 gagtgctgac gcgacgaaaa aaggtggcgc gaaaacgaaa ggccgtggac gtaaggccgc
      601 gcagccggag cctcagcagc cgtctgaacc cgcgtatgac ttcgatgacg atattccgtt
      661 ctgaacgggc tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg gagaaatgag
      721 gatgagcgaa tatttcagaa tacttcaggg actgccggac ggctccttta cccgcgaaca
      781 ggcggaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgatc agggaacgca
      841 ttttcgcctg gttgtccgtc aggatggcac gttgatctgg cgctcctgga attttgagga
      901 ctgtgccggg tactggatga accggtacat cagggatttc gggatcctta agtaagagag
      961 gtgccggacg gggatcacgt ccggcagaca ttaactacac aataagggga tagtcatgtc
     1021 agtaacaaag tctgaaccaa aatcta
//
LOCUS       DAZGXZ010000380         1045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000380 DAZGXZ010000000
VERSION     DAZGXZ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.383"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..563
                     /locus_tag="ACHKJF_005605"
     CDS             30..563
                     /locus_tag="ACHKJF_005605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0952748.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            560..1045
                     /locus_tag="ACHKJF_005606"
                     /pseudo
     CDS             560..1045
                     /locus_tag="ACHKJF_005606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
BASE COUNT          300 a          226 c          274 g          245 t
ORIGIN      
        1 aagcacgagc gtcctcatca taaatatata tgaacacaaa aatcagatac ggcctgtcgg
       61 ctgccgttct ggcgctgatt ggtgctggcg catctgctcc tcagatactt gaccagtttc
      121 tggacgaaaa agaaggtaac cacacaatgg cataccgcga tggttctggc atatggacca
      181 tctgtcgggg tgccacagtg gtggatggaa aaaccgtttt tcccaatatg aaactgtcga
      241 aggaaaaatg cgaccaggtc aacgccattg agcgtgataa ggcgctggca tgggtggagc
      301 gcaatattaa agtaccactg accgaaccac aaaaagcggg tatcgcgtca ttttgtccct
      361 ataacattgg ccccggtaag tgtttcccgt cgacgtttta taagcggctg aatgctggtg
      421 atcgtaaagg tgcatgcgaa gcgattcgct ggtggattaa ggatggcgga cgcgattgcc
      481 gcattcgttc aaataactgt tacggtcagg ttattcgtcg tgaccaggag agcgcattaa
      541 cctgctgggg gatagaacag tgaatcagat attcatggtg atttttctcg tgttgtcagg
      601 atttatcgtc ggaaatgtct ggagcgacag aggatggcaa aaaaaaatgg gcggaacgtg
      661 atgctgccgc attatcacaa gaggtaaatg ctcaatttgc tgctcgaata attgaacagg
      721 ggcgaactat agcccgtgat gaggctgtta aagatgcaca acagaaatct gctgaaattt
      781 ctgccagggc tgcttatctg tctgatagtg ttaaccagtt gcgtgccgaa gcaaaaaaat
      841 atgccatacg ccttgacgca gcgaagcata ccgcagatct tgccgctgcc gtcagaggca
      901 aaacaaccaa aaccgccgaa ggaatgctca ccaacatgct cggagatatt gcagcagaag
      961 ctcagcttta tgctgaaatt gctgacgaac gctacatcgc aggagtgact tgtcaacaga
     1021 tctatgaatc tttaagagat aaaaa
//
LOCUS       DAZGXZ010000381         1045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000381 DAZGXZ010000000
VERSION     DAZGXZ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.384"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..186
                     /locus_tag="ACHKJF_005607"
                     /pseudo
     CDS             1..186
                     /locus_tag="ACHKJF_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            411..902
                     /locus_tag="ACHKJF_005608"
     CDS             411..902
                     /locus_tag="ACHKJF_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0952749.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCKIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
BASE COUNT          414 a          167 c          156 g          308 t
ORIGIN      
        1 caaaatagag aataatgtag acctgaataa attcactact atatgcacag acgttctatc
       61 cccccgcgtc accagacata ataaagaaaa aaaacaaacg acattctacc cttctcaaaa
      121 atcccctttt taattttcat tgaaaaaatg gcaattgata ctatgaatcc aggtcataca
      181 cattaaggca tttatgtaat gtcgtaatta agataactaa taaggtgaat attagtaaaa
      241 caactaatga tgttattagt ttgtagtgaa cagtactttt accaataatg aaaaatatac
      301 cataagcaat gcaagcttaa aactcataat ttaaaaaaga taaatataaa aatatcaatg
      361 agtcaatcaa taataatttg gcatcacgag acacatcaca gaggaatatt atgagcacag
      421 aaacaattga aatattcaat aatagtgatg aatgggcaaa tcaactaaaa cacgcattat
      481 cgaaaggaga aaatctggca ttactacatg gtttaactcc tgatatcctt gatagaatat
      541 atgcatatgc attcgactac catgaaaagg gtaatataac ggacgcagaa atttattata
      601 aatttttgtg catttatgcg ttcgaaaatc atgagtatct aaaagatttt gcatcagtat
      661 gtcagcccaa aaagaaatat caacaagcat atgaccttta caaactaagt tacaattact
      721 ccccgtatga tgactattca gttatttatc gtatgggtca atgtaagatt ggggctaaaa
      781 atatcgataa cgcaatgcaa tgtttctatc acattattaa caattgtgag gatgatagtg
      841 ttaagagtaa agcgcaggca tatattgaac tcttaaacga taattcagaa gataatggct
      901 aagcattaca atcatttggt taaatttgct ggttatagtg cagttaacca ctataaccag
      961 cacctcttat gttttcttaa atcagcatct taaaacagtt aagagcatac tataaatcat
     1021 tatttttcaa tgcgttagag aacaa
//
LOCUS       DAZGXZ010000382         1030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000382 DAZGXZ010000000
VERSION     DAZGXZ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.385"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            329..961
                     /locus_tag="ACHKJF_005609"
                     /pseudo
     CDS             329..961
                     /locus_tag="ACHKJF_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          346 a          142 c          198 g          344 t
ORIGIN      
        1 gttaacgaaa tctgcgaaca agccagacat tatttagttg aaaaaatgtt tgaaacacat
       61 tctttggata tgaataattc tgttttggca agccctgagg acttatagct ccctggctca
      121 agtttaatga tttgaatata atctaattgt aatattttac tcatctcatg attttttaat
      181 gcctatggta ttttttacgc aaaaatttta tttttaatta tatttcatta ttatttcatc
      241 aatgtattct ttggattttg cttataataa taatggaagg gatgattata aatgaatgcg
      301 ttaaagcatt tacactcagg atgaatatat gggaaaaatt aaaattgtag tttcagatca
      361 gcagccgttt atgattgatg ggataattgg atttctcgga cattatcctg atttatatga
      421 ggttgttggg ggctataaag atctgaagaa agctatagcg gagtgtaaca aatcttcagc
      481 ctaaatattt attttaggag aatttgcagg tggaatgatg gggtccgaac tcgtaaaatg
      541 ggttaaatcg cataagatag atgctcatat tattacattt gtagcaaaga tgccgtatat
      601 tgattcaata aaattgcttg aagcaggtgc gaaaggatgc gtatggaaaa ccagtcaccc
      661 ggcgaaacta aatcgtgcta ttgattcgat tagtaatggt tacacttatt ttgatagtgt
      721 acatatggat tgtgaaaaaa tatcttccag gtattcctct gataatcaac ttacaaatcg
      781 tgagtcagaa atattacaac tcatagctga tggtaaaaca aacaaagaaa tcgctaattt
      841 cttgcagtta agcaggaaaa cagtcgaaac tcatagactc aatatcatga agaaattaga
      901 tgttcacagt ggtatcgagt tgatcaaaac agctttacgt atgggtgtgt gcactatata
      961 aaaaaaagag gctttaggct cattttgcta aagccttacg attgtctagt tcattaagcc
     1021 tggtatatac
//
LOCUS       DAZGXZ010000383         1005 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000383 DAZGXZ010000000
VERSION     DAZGXZ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1005
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.386"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..910)
                     /locus_tag="ACHKJF_005610"
     CDS             complement(<1..910)
                     /locus_tag="ACHKJF_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0952750.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDV"
BASE COUNT          259 a          313 c          212 g          221 t
ORIGIN      
        1 tcacatcccc tgcaggaatc accgtaaacc agttcgtctt cccgttctca ccggcataac
       61 catgaagtac agcaacaaat acccactgct tcttcaccgc atcccaggca aaaagcggtg
      121 agccactgtc tcctgatgtc ccgtaagacg ccagcggacc attaacaccc tggtaaagat
      181 ttcccgggtt tgacaccaga ctccagtcag aaatgaccgg acttcccacc gtcccgccag
      241 tcctccagac gtaagcatcc gctattctgg tcagcgcacc ggaagcagct ccggtgtact
      301 gagttccact ccccatacga taaaacaccg gataacgact tcggttctgg tataccccct
      361 tgccggttcc ggcatccgtg acgctcaccg gggcaacttc tgtcaccagt ttgttcagcc
      421 ggggagtatg aaaatcacgc cagtgctcat tacggtcaac aaggcgatat gtcgtatcgt
      481 ccgcataacc aaaacgcacc gtattgtagc tgccgttatg cttaaccccc gccacatact
      541 gcggagaaat cagtgttgcc acccccagta tctggtccac aacactgaaa tccatcatcg
      601 gagccttgtc cagccgccca ctgacagccc cattcttacc ataaagcgga atatttgtgg
      661 ccccggcatg aaacgcccct ttgttttcag caaaatccct gtaggtctgc catggcagag
      721 tattcccgac aacggatgcc agcgcaggag aagaaaacag aacgccggca acggctcctg
      781 aggccagaag aagacgggaa agcatccccg caccgggaat tccggacgac tttttcctgc
      841 cccccaccgt ttccggtaca gcaaccagtt cttttctgtc attaactctg agtgagtata
      901 ttttgttcat tccctggaat tccctgtatt ttctcccgga caacaataaa cagtgggcac
      961 aatcatcata ctaaaacaaa ctttgactga taaagatgaa gtcag
//
LOCUS       DAZGXZ010000384          947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000384 DAZGXZ010000000
VERSION     DAZGXZ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.387"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            177..>947
                     /locus_tag="ACHKJF_005611"
     CDS             177..>947
                     /locus_tag="ACHKJF_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP0952751.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGMGEATIR"
BASE COUNT          259 a          175 c          241 g          272 t
ORIGIN      
        1 gaactatgca atttttaatc tatcaattcg aaagcattag caaattagcc aaattgacac
       61 cttcccacct ccaactaaca ctcaatacag acctctgaca atttaataag tttcattttt
      121 aatgtctgac gactcatctc tttcgtggct gtattgtttg tatatggagc gtttatatga
      181 atagaatcta tcgcgtgata tggaattgca ctctacaggt atttcaggcc tgctcggaat
      241 taactcgcag ggcaggtaaa acatcgacgg ttaatttgcg taaatcctct ggactgacaa
      301 cgaaattcag tagattgacg ctgggtgttt tgctggcact aagcggttca gcgtctggtg
      361 caagtctgga agttgataat gatcagatta ccaatattga tactgatgtt gcttatgatg
      421 cctacctggt tggctggtat ggcactggag tgcttaatat tttggctggc ggtaatgcct
      481 ccttaaccac tattactacc agcgtcattg gcgctaatga ggactcagag ggcaccgtta
      541 atgttttggg tggcacctgg cgattgtatg atagcggaaa taatgcaagg cctttaaatg
      601 tgggtcaatc cggaacgggg acgctgaata ttaaacagaa gggtcacgtc gatggaggct
      661 atttaagatt aggttcttcg acaggaggcg tcgggacggt caatgttgag ggagaggact
      721 ctgttctgac gaccgaatta ttcgaaatag ggagttacgg tacgggctca ttaaatatta
      781 cggataaggg ttacgtcacg agttcaatcg tcgccatttt aggctatcag gcgggcagta
      841 atggccaggt tgtcgttgaa aagggtggcg agtggctaat aaaaaataac gattcctcaa
      901 ttgaatttca aattggtaat caagggatgg gggaagcgac tattcgc
//
LOCUS       DAZGXZ010000385          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000385 DAZGXZ010000000
VERSION     DAZGXZ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.388"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..409
                     /locus_tag="ACHKJF_005612"
     CDS             <1..409
                     /locus_tag="ACHKJF_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0952752.1"
                     /translation="VVEQVKDWREAVAISCRPLIENGSIEPRYVDAIYHSHDTIGPYY
                     VVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIVLAATDSTSHIEA
                     ISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            425..712
                     /locus_tag="ACHKJF_005613"
     CDS             425..712
                     /locus_tag="ACHKJF_005613"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0952753.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLVAQSVAGKIVALENMIDKKAMRERLSVALTELGAL"
     gene            725..>936
                     /locus_tag="ACHKJF_005614"
     CDS             725..>936
                     /locus_tag="ACHKJF_005614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP0952754.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLSDFANIFQ"
BASE COUNT          240 a          204 c          249 g          243 t
ORIGIN      
        1 ggtcgtggaa caggtcaaag actggcggga agccgtggcg atttcatgcc gaccgttgat
       61 tgaaaacggc tctatcgaac cgcgttatgt tgatgcgatc tatcactctc acgacactat
      121 cggtccgtac tatgtggttg gccctggtat tgcgatgcct catgcgcgcc cggaagaggg
      181 ggctaacaag ctgtcattag cactgaccct gatcccttca ggtgtgaatt ttgatgccga
      241 tgaaaacgat ccagtgaaat tactgattgt gctggctgca accgacagta ccagccacat
      301 tgaagcgatt tctcaactcg ccaagttatt cgataacgaa aaagacatac aggccatttt
      361 gacggccaaa acgacgcagg acattttgtc tgttattgcg cgctattaat cctgaggaac
      421 atttatgaaa attacagtgg tttgcggtaa cggcttaggc accagcctga tgatggaaat
      481 gagcattaaa agcattttga aggatttgtc cgtcagcgct gatgttgatc atgtcgacct
      541 gggctccgcg aaagggacgc cgagtgatat ctatgtcggc accaaagaca ttgccgagca
      601 gttagtcgcg cagtctgtcg caggcaaaat cgtcgcactg gaaaatatga ttgataagaa
      661 agccatgaga gaacgtttat ccgtggcatt aaccgaatta ggcgcactgt aagctggggg
      721 cgatatggat ttctttcgtt ttttgatgag cgatgtactt tccgaacccg cagtgctggt
      781 cggtttaatc gccctgattg gcctgattgc acagaaaaaa ccggtaaccg aatgcattaa
      841 aggtaccgtc aagaccatta tggggtttgt gattttaggc gcaggggcgg gtttagttgt
      901 gtcgtcactg agtgacttcg caaacatttt ccagta
//
LOCUS       DAZGXZ010000386          933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000386 DAZGXZ010000000
VERSION     DAZGXZ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.389"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..538
                     /locus_tag="ACHKJF_005615"
     CDS             <1..538
                     /locus_tag="ACHKJF_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HFP0952755.1"
                     /translation="ATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDTGPAGP
                     AGPQGPKGDTGAAGPAGAQGPKGDKGDPGEAGPAGLAGPKGERGEPGPMGPPGKDGAT
                     SLVSGLRIGGKKEVAHHEVITFPSLDGAQPTIPGMNIVPDGAVILNIRSQAYKITAVT
                     YGFLQVHNGSQWVTIKTE"
     gene            602..>933
                     /locus_tag="ACHKJF_005616"
     CDS             602..>933
                     /locus_tag="ACHKJF_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0952756.1"
                     /translation="MIIKNLKHYTPEKPDVPGAMYLKSEDGQDWYECQSLFSAETLKL
                     VYDSNGIITSISKDVSMLWPVNQSVAEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDE"
BASE COUNT          262 a          198 c          299 g          174 t
ORIGIN      
        1 agccacgccg aaggccgtga aggcggcaat ggatgtggca aatgaagcga aaacaaaggc
       61 agaagaggct gcagcaggag gtggtgttcc cggtccgaaa ggagagaaag gggacacggg
      121 gccagcaggt ccagcaggac cgcagggacc taaaggggat acgggagctg ctggcccggc
      181 aggcgcacag gggccaaaag gtgacaaagg cgatccggga gaagcaggtc cagcaggtct
      241 ggcaggtccc aaaggtgagc gcggtgaacc gggaccgatg gggcctccgg ggaaagatgg
      301 cgcaacttcg ctggttagtg ggctgcgtat cggagggaag aaagaagtcg cacaccatga
      361 ggtgattact ttcccgtcac tggatggagc acaaccaacg attcccggca tgaatatagt
      421 ccctgatggt gctgtaattc tgaatatccg gtcacaggct tacaaaatta ctgctgtaac
      481 ttatggcttt cttcaggttc acaatggaag ccagtgggta acgattaaaa cggaataacg
      541 tgactcacac tggttgttgc gttccattat gcccaaccgg gcattaaata cggagaataa
      601 tatgattatt aaaaacctga agcactatac ccctgaaaaa cctgatgtgc cgggtgcgat
      661 gtacctgaaa tcagaggatg gtcaggactg gtatgagtgt cagtcgttat tttcagcgga
      721 gacgctgaag ctggtttatg acagtaacgg tataatcacc agcataagta aggatgtctc
      781 tatgctgtgg ccggtaaacc agagtgtggc agaggttgca gatacggaag aaaaccgcaa
      841 agctgacatc tcggggcgct ggggttttga cggggagaaa atcacggacc tgctgaccgc
      901 ggagaaagcg cgcgggatga agggcgatga aat
//
LOCUS       DAZGXZ010000387          932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000387 DAZGXZ010000000
VERSION     DAZGXZ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.390"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>932
                     /locus_tag="ACHKJF_005617"
     CDS             <1..>932
                     /locus_tag="ACHKJF_005617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HFP0952757.1"
                     /translation="VLQPGGLRKTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDM
                     TGAWFWRVLWRFRPYYGQVILASFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSI
                     GVIAAILFECAAKLIRSYLTDIAGKKADLIISSALLRRVMAVKMSDRPASSGSYASNL
                     REYESVREFMTSATLLAFVDLPFILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQR
                     PLSRHINASMRESSQRQGLLVEALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVK
                     NTSNVIICFSTAVQQLNTVLLVILGTYLIHSDDP"
BASE COUNT          197 a          216 c          260 g          259 t
ORIGIN      
        1 tgtactccag cccgggggac tccgtaagac actttctcac gatgaattaa tggctgttta
       61 ttcaggttac tgctggtttg ttcagccaca ggcaacacag gatcgccgtt cggaactgcc
      121 ggagtatgac atgaccgggg catggttctg gcgggtactg tggcggttca ggccatatta
      181 cggacaggtc attcttgcga gttttatcat taactttctt gcgctggtca gttccctgta
      241 tgtcatgaac gtttatgacc gggtcatacc caatcgctcc tgggaaacgt tatggatgct
      301 gagtatcggg gtgattgcag ccattctgtt tgagtgtgct gcaaagttaa tccggagcta
      361 tctgacggat attgcgggta agaaggcaga cctgattatc agctcagcac tgttaaggcg
      421 ggtgatggca gtgaaaatgt cggacaggcc ggcgtcttcc ggttcatacg ccagcaattt
      481 gcgggaatat gagtctgtca gggagtttat gaccagcgcc acgctgctgg cttttgtgga
      541 tttgccgttt atcctgcttt tcatcggtgt catcgggctt gtggggggct ggcttgctgt
      601 tgttcctctt acactggttc cgctggtgat tctggcagga atattactgc agcgaccgct
      661 ttcgcgtcat attaatgcct ccatgcggga atcatcgcag cgccagggat tacttgtgga
      721 ggcactggac ggaatagaaa ccctgaagct gaataatgca gttaactggg tccagcgccg
      781 ctgggatgga tatacagcca gtgtcgcccg ctcgtccata cgggtaaaaa ataccagtaa
      841 tgtcataatc tgcttttcca cagcagttca gcaactgaat actgttctgc tggtcattct
      901 ggggacgtac cttattcaca gtgatgaccc ac
//
LOCUS       DAZGXZ010000388          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000388 DAZGXZ010000000
VERSION     DAZGXZ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.391"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..643
                     /locus_tag="ACHKJF_005618"
     CDS             <1..643
                     /locus_tag="ACHKJF_005618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP0952758.1"
                     /translation="QGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPDGRLTARNADI
                     SGHISANSGTLNNVTINQNCRILGKLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTI
                     TVRIYDDQPFDRQIVIPAVAFSGAKHEREHNDIYSSCRLMVKCNGRVLFERTALAESQ
                     VYSGVIDMPAGRGHMTLEFSVSAWLVNGWYPTASISDLLVVVMKKSTAGIGIS"
BASE COUNT          219 a          216 c          274 g          213 t
ORIGIN      
        1 gcaggggaat cagctgttca tgaacgatgt gttcctgaag cggttgtttg cagtgagcat
       61 cacctcgtcc ggcaatcccc cgacgttctc cctgacgccg gacggcaggc tgacggcccg
      121 taatgctgat atcagcggac atatcagtgc gaactcgggc acgctcaata atgtgaccat
      181 taaccagaac tgccggatac ttggaaaact gtctgccaac cagattgaag gcgatattgt
      241 taaaaccgtg gggaaggctt tcccgcggga ctcccgcgca ccggagcggt ggccatcggg
      301 gaccattacc gtcaggattt atgacgatca gccttttgac cggcaaattg tgatcccggc
      361 agtggctttc agcggcgcta aacatgagag agagcataac gatatttatt catcatgccg
      421 gctgatggtg aaatgtaacg gcagggtgct ttttgaaagg acggcgctgg ctgagtctca
      481 ggtgtattca ggggtgattg atatgcctgc aggaaggggg catatgacgc tggagttttc
      541 tgtgtctgcc tggctggtga atggatggta cccgacagcg agtattagcg atctgctggt
      601 tgtcgtgatg aagaagtcca cggcgggtat cggtatttcg tgatcatgag gagccggatt
      661 gccggaagga gacaaaaacc gtacattatg cgcgggtgcc tttggctgat ggccggaggg
      721 agcacctgaa ggccggatgt gaaaaggccc cggacaaaca ttcatgttta acccgaggcc
      781 tgacgttctt ccttcagcaa gaagaaggtt agcgcctctc cgtaaaagga gcaagcgtta
      841 tgtcgcaaaa accgttaaaa accaccgtga tttgtatcac ggtagtgctc attatctgga
      901 tcacccacat gttcactgtg cg
//
LOCUS       DAZGXZ010000389          921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000389 DAZGXZ010000000
VERSION     DAZGXZ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.392"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            145..>921
                     /locus_tag="ACHKJF_005619"
     CDS             145..>921
                     /locus_tag="ACHKJF_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0952759.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCL"
BASE COUNT          312 a          177 c          169 g          263 t
ORIGIN      
        1 acgtaaagtt ttacgttgca acattaaagc ctcatttcaa tcatcatgat aaatataaaa
       61 ttaatatata tttatgccgt aaataccaat atacttagca aactatgtga tctccatttc
      121 gattgattta gtgtttattg acgtatgtac tggattatta acgataatat cgagttctgg
      181 cctgagcacc gaaaattaat atcggtacat aacgccgatc ttaacgtcgt tctgacaacg
      241 ccagccagtc gatgtttatc acttctactt gaagcttttc ctgatgtggt tgcacaacaa
      301 gattttttca ccagagtctg ggaagaagaa ggtatgcgtg tgcctactaa cacgttatat
      361 cagaacatat ccattatcag acgcggattt cgcgctgttg gtgatactac ccactcgcta
      421 attgcaaccg tgccgagaag aggattcaag atccataatg acattaacat acaaaatcat
      481 gtaataaact cgtcaacaga cgcacataca cacaatgccc cacctgccat aaaagttaat
      541 gcgggataca aagagagcat tggtggcgca aagaatttca ataacaaaat cctcaaacat
      601 ataaaaagtc atctaattat gttgagcgca tttgtcatag gcgcatattc tgcatattgg
      661 ctatggaata ataatcaacc gaagccattt ttcaaggatt acaagactgt tgcggaaatt
      721 aatggctgcc attttaatgt aacagaagat acaattgacg ggttgaaaga gttcgataaa
      781 tataaaacac ggatactgga ttcggggatt aactgtaaaa aacatccgtg gttatacttc
      841 cctcttgcaa aatcctcacc ggggatgatt gttatggcat gcaataaaaa ttataaccaa
      901 catgaagtgg cagactgttt a
//
LOCUS       DAZGXZ010000390          896 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000390 DAZGXZ010000000
VERSION     DAZGXZ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.393"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..276)
                     /locus_tag="ACHKJF_005620"
     CDS             complement(<1..276)
                     /locus_tag="ACHKJF_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP0952760.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFDLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPA"
     gene            complement(281..496)
                     /gene="essD"
                     /locus_tag="ACHKJF_005621"
     CDS             complement(281..496)
                     /gene="essD"
                     /locus_tag="ACHKJF_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP0952761.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            717..887
                     /gene="ynfU"
                     /locus_tag="ACHKJF_005622"
     CDS             717..887
                     /gene="ynfU"
                     /locus_tag="ACHKJF_005622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFP0952762.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
BASE COUNT          269 a          212 c          162 g          253 t
ORIGIN      
        1 ggcaggattt tcaatcttaa aggagtactg atgctgcaga taagactcaa ctttttctga
       61 caatttttct gctacttcca ggaagacttg ccggacgctc cttctggctg ctgcctcata
      121 aaactccagc gcagctcctt caacacggtc catggcgaca tccaggtcaa aaatttcacc
      181 gtcaaagcgt tccttgtcct gtaaggctac agttaccgta actttattct caaaattacg
      241 gactcctttc acaaccagtt catagtcttg agtcattgga ttactctcct ctcgcagcct
      301 tacgcctgtc ttctttaatc ttgaaataaa gatttgtcag atacgtcagc aggccaaaaa
      361 ccaggctacc cagcacaccg attgcagccc actgtgacgg agttacttta tcgagtaact
      421 gtaatgccca gaaaccagca ttacccgccg atgtgccata ggcaacacct gttgttaact
      481 tatccattga tttcatatcc tcaccccgat gtacacggat ggtgcaatat gtttgaaaaa
      541 gatcggagtc tacggggtag ttttgacagc acacgttgtt ctcaacggcg ctaaagaaac
      601 atacacatta aaaatgtgag taattatttt gaaagaaagt catatataaa ataataatac
      661 gagaaatgtt ttcatattta gtgtactgta tacggccatt tatacaggaa aagcctatgt
      721 cagaacgtaa agactcaaaa tcacgccgta attatctcgt taaatgttcc tgcccaaact
      781 gcacccaaga gtcagaacac agtttttcaa gagtacaaaa aggtgccctt ttgatctgcc
      841 ctcattgcaa caaagtattc cagacaaatc ttaaagctgt agcctgattg atttta
//
LOCUS       DAZGXZ010000391          890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000391 DAZGXZ010000000
VERSION     DAZGXZ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.394"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..150
                     /locus_tag="ACHKJF_005623"
     CDS             1..150
                     /locus_tag="ACHKJF_005623"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0952763.1"
                     /translation="MGVGGYGGESAIAIGVSRISDNGKMIMKLNAGQNTRGNFSVGAG
                     VGWQW"
     gene            175..>890
                     /locus_tag="ACHKJF_005624"
     CDS             175..>890
                     /locus_tag="ACHKJF_005624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0952764.1"
                     /translation="MLFSRMMMTGVLSLMLAGCGNLSHVTDEGTTEEVVWPSPEKVMF
                     NHDGTQKGSWPLPEKLRQIKAGMNKDQIYQLAGRPHFAEGMSGVREWDYLFNFTGPEG
                     DYTCQYKILFDRNMDARSFYWKPENCLPEAETAEYRESVPGDILFDFDSWTLTERGRD
                     ALSRVAEKFQAGHVVGVKIEGFTDHLGGQAYNRLLSEKRAESVRGWFIARGFRAERIH
                     ATGRGYDAPVVFCPDVEGEAL"
BASE COUNT          227 a          164 c          294 g          205 t
ORIGIN      
        1 ctgggtgtcg gtggatatgg tggtgaaagc gccattgcaa taggtgtgtc ccgaatctcc
       61 gataacggta agatgataat gaaactgaat gcaggtcaga acacccgggg taatttctcc
      121 gtcggtgccg gtgtgggctg gcagtggtaa cagggaaaag caaggagaaa tgtaatgtta
      181 tttagtcgaa tgatgatgac gggtgtgctg tccctgatgc ttgcaggatg tggaaatctg
      241 agtcatgtca cggatgaagg aacgacggaa gaagtggtat ggccttctcc ggagaaggtg
      301 atgtttaatc atgacggaac acagaaaggt tcgtggccac tgccagaaaa gttacgtcag
      361 ataaaggcgg gtatgaataa ggaccagatt tatcagctgg cggggagacc acactttgct
      421 gaagggatgt ctggtgtaag ggaatgggat tacctgttca actttaccgg accggagggg
      481 gattatacct gccagtacaa gatactgttt gaccggaaca tggatgcccg ctctttctac
      541 tggaagccgg aaaactgtct gccggaggca gagacagcag aatacaggga gtcagtcccc
      601 ggtgatatat tatttgattt tgattcatgg actctgacag agagggggcg tgatgcactt
      661 tcccgtgtgg cagagaagtt tcaggcgggt catgttgtcg gggtgaagat agaaggattt
      721 acggaccatc ttggcgggca ggcgtataac cggctcctgt cagagaaacg ggcggaatca
      781 gtcagggggt ggtttattgc ccggggattc agggctgaaa ggatacatgc aacgggccgt
      841 ggatatgatg caccagtggt gttctgcccg gatgtggagg gcgaagcact
//
LOCUS       DAZGXZ010000392          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000392 DAZGXZ010000000
VERSION     DAZGXZ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.395"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..534)
                     /locus_tag="ACHKJF_005625"
     CDS             complement(<1..534)
                     /locus_tag="ACHKJF_005625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952765.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEI"
     gene            complement(686..>883)
                     /locus_tag="ACHKJF_005626"
     CDS             complement(686..>883)
                     /locus_tag="ACHKJF_005626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0952766.1"
                     /translation="CYDNACVESFFHSLKVECIHGEHFISREIMRATVFNYIECDYNR
                     WRRHSWCGGLSPEQFENKNLA"
BASE COUNT          249 a          202 c          145 g          287 t
ORIGIN      
        1 aatttcactg cgcaacgcat ctaagtttgt aagatcactg tctataattt taagatagtt
       61 taaatctaac gaaccatcag ttttatcaag cttagttttc agccttttaa cagcctccaa
      121 ggaagcatca agaactggtg tctgattctt caaagcttct gaaaaacttt tttgtgttga
      181 ttttcgagct tgttgttgag cagattttct taactctttt tcacgcttaa cttgttctaa
      241 aagctcttta cggtcatcag aacgtgaacc aaaaaatcta tcagcaagtt cagttaacaa
      301 attagatata atagttttca gttctcttgc agcctggttt cttatgaatc cctctctccc
      361 cgacttatct ttcagctctt tattactgga ttgagtaatt ccaatataac caaaaatcct
      421 tctattagac caataatatc gccctgcatt ccatgaacgt ctttcttcta tctggaagaa
      481 atcattatct actcgaccat aaggtaatac tctcaagcta tccctaaaaa tcattagtcc
      541 tgcatacttt ttggccttaa gatcaaagtg ggaatgttca cgttcagtat gtgatgtatt
      601 ttgtgaaagg aattcaaacg ttcctatctg aagctcaaat gggccgaccc ctgcgtgatc
      661 ctacccacgt aatatggaca caggcctaag cgaggttctt gttttcaaat tgttccggac
      721 tgaggccgcc acaccaactg tgccgccgcc accgattgta atcacattcg atataattaa
      781 acaccgttgc ccgcattatt tcccggctga taaagtgttc tccatggata cattccactt
      841 tcagcgaatg aaagaagctt tccacgcagg cattatcgta gca
//
LOCUS       DAZGXZ010000393          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000393 DAZGXZ010000000
VERSION     DAZGXZ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.396"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..246
                     /locus_tag="ACHKJF_005627"
     CDS             <1..246
                     /locus_tag="ACHKJF_005627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0952767.1"
                     /translation="ASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGN
                     PMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          270 a          159 c          248 g          206 t
ORIGIN      
        1 gccagcatga tactgggtgg tgtggctcag atgctggccc cgaaggcaaa gacgccggag
       61 tataaaagta cggataacgg taaacagaac acgtattttt cgtcactgga caacatgatt
      121 gcccagggta acccgatgcc ggtgccttac ggtgaaatgc tggttggttc acgacggata
      181 tcccaggata tcagcacccg tgatgagggc ggagacggga aagtggtggt tatcgggcgg
      241 ggataaaaat aaaaaaaatc ccgcagagtt agtggagctg cgggaaataa cgatgaagat
      301 taatgttatg gagttatttt tttgggcaga aaaacattaa cgcagcgaaa ttataagcgc
      361 tacatggatt ttgcgaaaat gtgaagaaat tcagaaattt tttgctgcag aggtgcaggg
      421 agcttgccgg gtatctgtgg cagaagacag aaaacatcat gccggggtta agggataaaa
      481 aaatcccgca gagttagcgg agctgcggga cagaaccgat gaagattaac gttcaaggag
      541 ttttgttttt attgacccga aaaaactgta acgccccgga atgatatctg ccatggggac
      601 gcacaggaaa tgtgaagaaa ttcagacttt ttctgtcgca ggtttacaga acggattacc
      661 gccggggtac cagtggcgta aatgagccgg atgttgtggt gcggctgaag caaaaaaatc
      721 tcccgtaata tcgccggata ctgacgggag acgaatagtc accttaagga ggtctgataa
      781 ttattgcttt tattgagcaa aaaaactgta acgctgcatc attatagcgg ctacaggcaa
      841 ttgccggaaa tgtgaagaat ttcagaaatt ttattccgtc atg
//
LOCUS       DAZGXZ010000394          882 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000394 DAZGXZ010000000
VERSION     DAZGXZ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.397"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(165..>882)
                     /locus_tag="ACHKJF_005628"
     CDS             complement(165..>882)
                     /locus_tag="ACHKJF_005628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952768.1"
                     /translation="QGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTARNADI
                     SGHISANSGTLNNVTIAENCVVKGTISADRIIGDIVKGYSFEPRDGTLHITNHKYGEG
                     YVIFKAEVSLADDVSFDRKLILLPFFFYSGTLTIEINGRVVIKAEPRIADTGFSGRFD
                     RNGNAVNTYTNLGDTVSGVWDVVAGGGDTRITVTMRVEGGPGNMGDVEGRKRELVRWR
                     YDAFTLMVAKSTDSGFTRRS"
BASE COUNT          201 a          258 c          193 g          230 t
ORIGIN      
        1 cacttgctta ggatgaatgg tcaggcctcg ggttaaacat gaatgtttgt ccggggcctt
       61 ttcacatccg gccttcaggt gttccctccg gccatcagcc aaaggcaccc gcgcatactg
      121 tacggttttt gtctccttcc ggcaatccgt attccggctg cctgtcacga tctccgggta
      181 aaaccggaat ctgtcgattt cgccaccatg agagtaaagg cgtcatatct ccaccgcacc
      241 agctctctct ttctcccttc cacatccccc atattgccag gaccaccttc aactctcatt
      301 gtcactgtaa tccgtgtatc tccccctcct gccaccacat cccatactcc tgaaacggtg
      361 tccccgaggt tggtgtacgt gttaacagca ttgccatttc tgtcaaaacg acctgaaaaa
      421 cccgtatcag caattctcgg ttctgcctta atgacaactc ttccgttaat ctctatcgtc
      481 agtgttcctg aatagaaaaa gaagggaagg agtatcagct tgcgatcaaa cgaaacatca
      541 tcagcaagag atacctccgc cttaaagatg acataaccct ctccatactt gtggttcgta
      601 atatggagcg tgccatcacg aggttcgaag gaatagccct tgacgatatc accgatgatt
      661 ctgtctgcgc taatggttcc tttaacaacg cagttttctg caatggtcac gttattcagc
      721 gtgcccgagt tcgcactgat atgtccgctg atgtcagcat tgcgggccgt cagcctgccc
      781 tccggcgtca gggagaacgt cgggggattg ccggacgagg tgatgcttac tgcaaacagt
      841 cgcttcagga acacatcgtt catgaacagc tgattcccct gc
//
LOCUS       DAZGXZ010000395          863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000395 DAZGXZ010000000
VERSION     DAZGXZ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.398"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            499..684
                     /locus_tag="ACHKJF_005629"
     CDS             499..684
                     /locus_tag="ACHKJF_005629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0952769.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPVQAPCVKPPPPPAWIMQPA
                     PDWQTQLNGIISPSESG"
BASE COUNT          221 a          204 c          244 g          194 t
ORIGIN      
        1 atgacgttgg tcaacgcgga cggataacac gaaatcctgc gaactggcaa aacctaagtg
       61 aataaaagta aaaaacccgt ttgttagcag caggtggggt ttcgtgtttc ctgactccgg
      121 aaaagtcaaa ggagaaagtg tgtttgattt tagcaaattg attcgggaga tttgagtgat
      181 ggctgaaaaa ttatccacct ggaagttcat ccttatctgg ctggtgtttg tgattatggc
      241 ccccggttat ttcatcggtc agatacgctg gtggtgaaat gaaccgcgtt ctgtgtgtgg
      301 tcatcattgc cctgctggtg gcctgtggtg cgcttagtct ggggctgaat cattaccgcg
      361 ataacgccat cacctacaaa gagcagcgcg ataaaaaagc cagagagctg gagctggcaa
      421 acgcaaccat tactgatatg cagcagcgcc agcgcgatgt tgctgcgctc gatgcaaaat
      481 actcgaggga attagccgat gcgagagctg aaaatgaaac tctgcgtgct gatgttgccg
      541 ctggtcgtaa gcgcctgcgg atcaacgcca cctgtccagg ctccgtgcgt gaagccccca
      601 ccacctccgg cgtggataat gcaaccggcc cccgactggc agacacagtt gaacgggatt
      661 atttcaccct cagagagcgg ttgatgatga tgcagaagca gctggaaggg gcgcaggaat
      721 atatccgcac tcagtgcctg aaataagttt tgctgatgcg ccgtatcgtc gccgtattcc
      781 cgtattaaca gagaccgcag cccgacaggg agactcctct gcgagagtgt gcggggataa
      841 tcaaaaacga tacacaccgg ggt
//
LOCUS       DAZGXZ010000396          858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000396 DAZGXZ010000000
VERSION     DAZGXZ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.399"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            80..712
                     /gene="yqeH"
                     /locus_tag="ACHKJF_005630"
     CDS             80..712
                     /gene="yqeH"
                     /locus_tag="ACHKJF_005630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0952770.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          280 a          149 c          166 g          263 t
ORIGIN      
        1 attacaaaat catggtgtat tgacatcatc aatcaccaat gaaattatgc aattatatac
       61 ggatagggag gttcttaaca tggggctttg tagtcgttat aaaagtctta catgcaatag
      121 ttgctcaatg cattgccaaa taatgccaga agagtcaccg cgtttgcagt actgcgcaaa
      181 ctcgtgtttt tgtatgtggc ccgaagaaag ttcatatttt aatcgtggtg tggtagaagg
      241 tatcttaaca aaaaaccaca atgcgagatt aagcggatat atttttgtcg atttttcagt
      301 aagtttttta cgtctattcc ttgaaaaaga ctggattgac tatcttgcaa gtactgacat
      361 ggggattgtt ttagtcagcg accgcaatat gcaatcgtta gctaattact ggcgaaaaca
      421 caactcagca atatctgctg tcatatataa tgatgatggt cttgatgttg ccaatgaaaa
      481 gatcagacaa ctatttattg gtcgttattt atcatttacc agaggtaaca cattaactca
      541 gatggaattc accataatgg ggtatatggt ttctggttat aatccatatc agattgctga
      601 agttctggat atggatatcc gtagcatcta cgcgtacaag caacgaatcg aaaagagaat
      661 gggtggtaaa ataaacgaat tatttattcg ttcacattcg gtccaacatt gatactcaac
      721 gaccagccag aatcatactc tgtttatacg ggcatttatt gaaatgtccg tattttataa
      781 ataggccgtc ccctcaagag taaacaccat agtatttaaa accatcacac gaaaaattca
      841 gaagcattac gaaatatg
//
LOCUS       DAZGXZ010000397          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000397 DAZGXZ010000000
VERSION     DAZGXZ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.400"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>822
                     /locus_tag="ACHKJF_005631"
     CDS             <1..>822
                     /locus_tag="ACHKJF_005631"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFP0952771.1"
                     /translation="VDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISG
                     WLRRTKPRQKTLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERG
                     TINIVNGTPIHERSRNIDNRRSLGHWEGDLVSGTKNSHIATLVDRKSRYTIILRLRGK
                     DSVSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRG
                     TNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGV"
BASE COUNT          274 a          187 c          183 g          178 t
ORIGIN      
        1 ctgttgatgc taataaccga gccaacagaa tggcgaaaag gccaaaaccg tgcttactgg
       61 atcaaaattt accattgcga aagcttgttc tggaaaagct ggagatgaaa tggtctccag
      121 agcaaatatc aggatggtta aggcgaacaa aaccacgtca aaaaacgctg cgaatatcac
      181 ctgagacaat ttataaaacg ctgtactttc gtagccgtga agcgctacac cacctgaata
      241 tacagcatct gcgacggtcg catagccttc gccatggcag gcgtcatacc cgcaaaggcg
      301 aaagaggtac gattaacata gtgaacggaa caccaattca cgaacgttcc cgaaatatcg
      361 ataacagacg ctctctgggg cattgggagg gcgatttagt ctcaggtaca aaaaactctc
      421 atatagccac acttgtagac cgaaaatcac gttatacgat catccttaga ctcaggggca
      481 aagattctgt ctcagtaaat caggctctta ccgacaaatt cctgagttta ccgtcagaac
      541 tcagaaaatc actgacatgg gacagaggaa tggaactggc cagacatcta gaatttactg
      601 tcagcaccgg cgttaaagtt tacttctgcg atcctcagag tccttggcag cggggaacaa
      661 atgagaacac aaatgggcta attcggcagt actttcctaa aaagacatgt cttgcccaat
      721 atactcaaca tgaactagat ctggttgctg ctcagctaaa caacagaccg agaaagacac
      781 tgaagttcaa aacaccgaaa gagataattg aaaggggtgt tg
//
LOCUS       DAZGXZ010000398          819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000398 DAZGXZ010000000
VERSION     DAZGXZ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.401"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>819
                     /locus_tag="ACHKJF_005632"
     CDS             <1..>819
                     /locus_tag="ACHKJF_005632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952772.1"
                     /translation="QTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGG
                     YVDCVALDAALLEKLQAVAEHLREAEGWEWCAGRMESVGFCREDAGTYRCLPEPESVL
                     TEAEEERLNELMARYDALENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSW
                     RYGNVCVQRGVQLRSEDDPAADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAAL
                     MQQPDKSLALLAWTLCLNVFGSGAYSKPAQISLECEHYSLTSDAPSGKEGAAFMA"
BASE COUNT          180 a          200 c          280 g          159 t
ORIGIN      
        1 cagaccattc gtcgtctggt gaccgaaagt gaagtggcgg tggcaggcaa cagtaaattc
       61 cgcttcgtgg gggctgatgc tttctcacct gacgaactgc gcaccgattt gttcagcgac
      121 gacgagggtg gctatgtgga ctgtgttgcg ctcgatgctg ccctgctgga aaaactccag
      181 gctgtcgctg aacaccttcg ggaagctgaa ggctgggaat ggtgcgccgg acgtatggag
      241 tctgtcggtt tctgccgtga ggatgccgga acataccgtt gtctgccgga gccggaatcg
      301 gtgctgaccg aggcggaaga agaacgcctg aacgaactga tggcacgtta cgacgcactg
      361 gaaaaccagt gtgaggaatc tgacctgctg gcagcagaaa tgaagctgat tgactgcatg
      421 gcgaaggtca gggcgtggac gccggagatg cgtgccggaa gtggcgtggt ggtgtcctgg
      481 cgttatggca acgtatgtgt ccagcgtggt gtgcagttgc gcagcgagga tgacccggct
      541 gccgacgctg accgcacgga acaggtgcag gagaaagcgt cagtggagga aatcagtctg
      601 ccactgctga cgaaaatgtc ttcagagcgc acgctggcag tccaggcggc gctgatgcaa
      661 cagccggaca aatccctggc actgctggca tggacgctct gcctgaatgt gtttggcagc
      721 ggggcgtaca gtaaaccagc acaaatcagc ctggaatgtg aacattattc gctgaccagc
      781 gatgcgccat cggggaagga aggtgccgca ttcatggcg
//
LOCUS       DAZGXZ010000399          814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000399 DAZGXZ010000000
VERSION     DAZGXZ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.402"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            94..>814
                     /locus_tag="ACHKJF_005633"
     CDS             94..>814
                     /locus_tag="ACHKJF_005633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFP0952773.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYE"
BASE COUNT          206 a          206 c          223 g          179 t
ORIGIN      
        1 atgcagcgga ctgcctttct cccaaagtga taaaccggac agtatcatgg accggttttc
       61 ccggtaatcc gtatttgcaa ggttggtttc actatggaac atgaacttca ttatatcggt
      121 atcgacaccg ctaaagagaa actggatgtc gatgtgttgc gtcctgatgg tcgtcatcgc
      181 accaaaaaat tcgctaacac cactaaaggg cacgatgagc tggtgagctg gctgaaaggt
      241 cacaagattg accatgcgca tatctgcatc gaagcgaccg gcacctatat ggaacctgtc
      301 gctgagtgcc tttacgatgc tggctacata gtgtcagtca ttaatcctgc gctgggtaaa
      361 gctttcgctc agagtgaagg actgcgtaac aagactgata ccgtggatgc gcgcatgctg
      421 gcagagttct gtcgtcagaa gcgccctgca gcctgggaag cgcctcaccc gcttgaacgc
      481 gcgttgcgtg ccctggtagt ccgccaccag gcgctgacag atatgcacac gcaggaactg
      541 aatcgcactg aaacggcgcg ggaagtccag agaccgagca ttgatgctca ccttctgtgg
      601 cttgaagcag agctgaagcg tcttgagaag cagataaaag acctgacaga cgatgatccg
      661 gatatgaaac accgcaggaa actgctggaa agcatcccgg gtatcggaga gaaaacatct
      721 gcggtattgc tggcttatat cggtctgaag gaccgcttcg cccatgccag acagttcgcc
      781 gcttttgcgg gtctgacacc acggcgttat gaat
//
LOCUS       DAZGXZ010000400          808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000400 DAZGXZ010000000
VERSION     DAZGXZ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.403"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..725)
                     /locus_tag="ACHKJF_005634"
     CDS             complement(<1..725)
                     /locus_tag="ACHKJF_005634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0952774.1"
                     /translation="MSAYVNPKASNGVAVGSWAKADALNSVALGTGAQAMHRYSVALG
                     QQSGTTRENEVYIGYDSGVTNPASPRVPDKTRVLGGVSDGTRDTDAATVGQLNRKADE
                     VYSDVSGRIAAEALKARDHTDTVAAENRENIIRNTVAINRNTRGLLSQRDLLETHEER
                     LNSQQQQINTGSTVAVDSHGYVMRGEGTGERITVQEGLVRTQEMATENRAAVSRNRQV
                     GERNSRAIASQARTLQAHERRMN"
BASE COUNT          159 a          252 c          181 g          216 t
ORIGIN      
        1 ctgttcatac gacgctcatg cgcctgcagg gtacgtgcct gagaagcaat ggcgcgactg
       61 ttgcgttcac ccacctgacg gttacggctg accgcagcac ggttttctgt ggccatttcc
      121 tgtgtacgta ccagcccttc ctgtacagta attcgttcac cggttccctc accgcgcatc
      181 acataaccat gactgtccac agcaacggta cttccggtat ttatctgctg ttgctggctg
      241 ttcaggcgct cttcatgcgt ctccagcagg tctctctggg aaagcagtcc ccgggtgtta
      301 cggtttatgg ccaccgtgtt ccggataata ttctccctgt tctctgcagc aaccgtatcg
      361 gtatggtcac gcgccttaag tgcttccgct gcaatccgtc cactgacgtc actgtacacc
      421 tcatccgcct tcctgttcag ttgcccgaca gtggctgcat ccgtatcccg tgtaccatca
      481 ctgacaccac cgagaacccg ggttttatcc ggtacacgcg gactggccgg gtttgtgaca
      541 cctgagtcat atccgatgta gacttcattt tcacgggttg taccggactg ctgaccaaga
      601 gccactgaat acctgtgcat tgcctgtgca cccgtaccca gtgccaccga atttaaagca
      661 tcagcctttg cccagctacc tactgccaca ccattagacg ccttcggatt tacgtaagct
      721 gacatcccca cggatgtggg gttctgcgcc catttcctcc aaaagtctca ctcttagcat
      781 aaaaaccatt atttatcatg ttgttaac
//
LOCUS       DAZGXZ010000401          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000401 DAZGXZ010000000
VERSION     DAZGXZ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.404"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(90..287)
                     /locus_tag="ACHKJF_005635"
     CDS             complement(90..287)
                     /locus_tag="ACHKJF_005635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0952775.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(500..>802)
                     /locus_tag="ACHKJF_005636"
     CDS             complement(500..>802)
                     /locus_tag="ACHKJF_005636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0952776.1"
                     /translation="KTQSGSRKIAVKTLERVKKLIWLAAQDVRGWVTGCEVYQRQELA
                     RLCGVKPDNWSHNYANYWREMCDIFKRLDRESLICSVKIRAQQKATFSRRDIAKVN"
BASE COUNT          206 a          219 c          159 g          218 t
ORIGIN      
        1 gtttgcttgt gatccggacg catgagcaga tggctggtga gtgcccctaa cacccaccag
       61 ccgcccattt accacaaata aaaaagcctt caggactgaa ggcgtctgta acaaccgaac
      121 tgatagtctg ccagccccgc cataacaagc tgggtcagta ttaactggca gcgttcacgt
      181 gaaagataag tattctgcgc aatctccccg actgtcgccg gttcagtgac gcttaattca
      241 ttaaacacca ctctggcggt ttcggtcata tcctgctgtt tcagcatgtc tttttccctt
      301 ttccggttaa cgtgacacac caataactct tgtcaaaaaa gccagcaagc tgaaagaccg
      361 gtattcacca ccaccagcgc gtttactgta ctgacgagat tttcggacat aaaaaaaacc
      421 acctggcggt ggttttttct tactttgcca tcgcgtacaa aatcggcaaa atatcagatt
      481 tatacgaaac atacgcgatt taattgactt ttgcaatatc tcgtcgtgaa aaggtcgctt
      541 tttgttgcgc tcttattttc acggagcaaa tcaaggattc tctatcgagg cgcttaaaaa
      601 tatcgcacat ctcacgccag tagttcgcat aattatggct ccagttatca ggcttaactc
      661 cacacagtct ggcaagctcc tgtctctggt agacctcaca cccggtaacc catcctctga
      721 catcctgtgc cgccagccag atcaacttct tcacacgctc cagcgttttc actgcaattt
      781 ttctggagcc ggactgagtt tt
//
LOCUS       DAZGXZ010000402          799 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000402 DAZGXZ010000000
VERSION     DAZGXZ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.405"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(86..241)
                     /gene="ynfN"
                     /locus_tag="ACHKJF_005637"
     CDS             complement(86..241)
                     /gene="ynfN"
                     /locus_tag="ACHKJF_005637"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFP0952777.1"
                     /translation="MREYPNGEKTHLTVMAAGFPSLTGDHKVIYVAADRHVTSEEILE
                     AAIRLLS"
     gene            complement(320..385)
                     /gene="ynfR"
                     /locus_tag="ACHKJF_005638"
     CDS             complement(320..385)
                     /gene="ynfR"
                     /locus_tag="ACHKJF_005638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFP0952778.1"
                     /translation="MKAPSGAFLLGIYSMDTHILR"
     gene            complement(388..576)
                     /gene="ynfQ"
                     /locus_tag="ACHKJF_005639"
     CDS             complement(388..576)
                     /gene="ynfQ"
                     /locus_tag="ACHKJF_005639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0952779.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(587..799)
                     /gene="cspI"
                     /locus_tag="ACHKJF_005640"
     CDS             complement(587..799)
                     /gene="cspI"
                     /locus_tag="ACHKJF_005640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFP0952780.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          254 a          166 c          139 g          240 t
ORIGIN      
        1 tcgcaataat caacatacga tagtctaaat tttacacaaa cagacaaaga gaattttcct
       61 gaattatcaa tacaatagca tcaaatcaac tcaagagcct tattgctgct tccagaattt
      121 cttctgaagt aacatgtcga tccgcggcta cataaatgac tttatgatct ccggtcagag
      181 atggaaaccc tgcggccatt acagtaaggt gtgttttttc gccatttgga tattcacgca
      241 tgatggtgtt aactccagtc atcgctggca ctaccactgc tggttcagag ttaaaaaaac
      301 tatgattttt ttcatgatgt taccgtagta tgtgagtatc catcgaatag ataccaagca
      361 aaaaagctcc cgaaggagcc ttcattttca cttttttaaa tccaacgaca gacggctggc
      421 atttaagtat tgtgaaatat tatcaaatgt aatcatcatt gatttacaaa agatacattt
      481 tgccccgaaa ggattcatgt cagaaacatc aaaagatgat gttctatact gggaaccatg
      541 acaacacggg catctaaagt gaatatggtt tgtaatattg tctacctcaa agcgccacta
      601 catgaacagc ggcaggacct ttaggtccgt tctcaatacc aaattcaact tcctgattct
      661 cagttaatgt tttgaaatcg ttgctctgaa ttgctgagaa atggacaaac acatctttgc
      721 tgccatcttt cggcgtgatg aaaccaaaac ctttttcagg gttaaaccat ttcactaaac
      781 cagtcatttt gttagacat
//
LOCUS       DAZGXZ010000403          796 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000403 DAZGXZ010000000
VERSION     DAZGXZ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.406"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(273..494)
                     /gene="traR"
                     /locus_tag="ACHKJF_005641"
     CDS             complement(273..494)
                     /gene="traR"
                     /locus_tag="ACHKJF_005641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFP0952781.1"
                     /translation="MSDEVDEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(629..>796)
                     /gene="traV"
                     /locus_tag="ACHKJF_005642"
     CDS             complement(629..>796)
                     /gene="traV"
                     /locus_tag="ACHKJF_005642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFP0952782.1"
                     /translation="VSSVAPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
BASE COUNT          206 a          198 c          168 g          224 t
ORIGIN      
        1 gggaagctca tttcgcccag aacttcattg gcctttgcgg attcgtcagg cagtttcagt
       61 gctgtgacga gggagttaac cgcctgagtg acggcctcaa gtgggttatt cacatgaatt
      121 ccttaattga aaaataaagc gagaaatgcg gtagccatga agggaatacc tgtaattaac
      181 gcgccatttc ccggcgcata tacaaagatt tctgttttct gaatatccac tcatgttcat
      241 ccggaattaa atcaatatgt tctgcgactg acttatgcat aatgttttct ctgtctctcc
      301 tgatacgcct gacattcaac gcacaacgtc acaccgggaa atattttcct cctggcttcc
      361 ggaataggat ttccgcatgc ctcacagaga taaacaggaa taccatgagc atttattttc
      421 tggcgtatcc ggtttattcc tgtcatggtc agttgttctg tcactgaata tgcttcatcg
      481 acttcatcac tcacggaata ttcctcccga catgttctga cacgctaccg cccatccctt
      541 ttcacgcaca gggcactcgt tttacaggca gattttgaat acagaaagcc cgccggcatt
      601 gccggctgat atacaatttc agggccagtt aattaatacg tggttttccc cacgcagacg
      661 gtttaataac aaaaaacaca ctggatggct gatgataaac atcctgatta tcaatataag
      721 gcgctatcca taatgctgcc gtctgctcac ccgttcttag cggacgggga ggcgttaccg
      781 gtgcaactga actgac
//
LOCUS       DAZGXZ010000404          789 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000404 DAZGXZ010000000
VERSION     DAZGXZ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.407"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            108..>789
                     /locus_tag="ACHKJF_005643"
     CDS             108..>789
                     /locus_tag="ACHKJF_005643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0952783.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDD"
BASE COUNT          260 a          132 c          171 g          226 t
ORIGIN      
        1 tttatttcta atagacgaga tttttcctgt tatgatataa tatgccgaat taacacatgt
       61 taacgattta ccagtaatgt aaataaattt tcgaggagat cattccagtg ggacgtaaat
      121 gggccaatat tgttgctaaa aaaacggcta aagacggtgc aacgtctaaa atttatgcaa
      181 aattcggtgt agaaatctat gctgctgcca aacaaggtga acccgatcca gaattaaaca
      241 catctttaaa attcgttatt gaacgtgcaa agcaggcaca agttccaaag cacgttattg
      301 ataaagcaat tgataaagcc aaaggcggcg gagatgaaac gttcgtacag ggacgttatg
      361 aaggctttgg tcctaatggc tcaatgatta ttgctgagac attgacttca aatgttaacc
      421 gtacgattgc taacgttcgc acaattttca ataaaaaagg cggcaatatc ggagcggcag
      481 gttctgtcag ctatatgttt gacaatacgg gtgtgattgt atttaaagga acagaccctg
      541 accatatttt tgaaattttg cttgaagctg aagttgatgt tcgtgatgta actgaagaag
      601 aaggtaatat tgttatttat actgaaccta ctgaccttca taaaggaatc gcggcgctaa
      661 aagcagctgg aatcactgag ttctcaacaa cagaattaga aatgattgct caatctgaag
      721 ttgagctttc cccagaagat ttagaaatct ttgaagggct tgttgatgcc cttgaagatg
      781 acgacgatg
//
LOCUS       DAZGXZ010000405          785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000405 DAZGXZ010000000
VERSION     DAZGXZ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.408"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..785
                     /locus_tag="ACHKJF_005644"
                     /pseudo
     CDS             1..785
                     /locus_tag="ACHKJF_005644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          181 a          213 c          236 g          155 t
ORIGIN      
        1 gcaaaatcag cagacgtctt ctgaacgtga actggagatg tctaccgaga ttgcacgtct
       61 caaacgccag ctggcagaac gggatgaaga gctggctatc ctccaaaagg ccgcgacata
      121 cttcgcgaag cgcctgaaat gaagtatgtc tttattgaaa aacatcaggc tgagttcagc
      181 atcaaagcaa tgtgccgcgt gctccgggtg gcccgcagcg gctggtatac gtggtgtcag
      241 cggcggacaa ggataagcac gcgtcagcag ttccgccaac actgcgacag cgttgtcctc
      301 gcggctttta cccggtcaaa acagcgttac ggtgccccac gcctgacgga tgaactgcgt
      361 gctcagggtt acccctttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcagggactg
      421 agggcaaagg cctcccggaa gttcagcccg gtcagctacc gcgcacacgg cctgcctgtg
      481 tcagaaaatc tgttggagca ggatttttac gccagtggcc cgaaccagaa gtgggcagga
      541 gacatcacgt acttacgtac agatgaaggc tggctgtatc tggcagtggt cattgacctg
      601 tggtcacgtg ccgttattgg ctggtcaatg tcgccacgca tgacggcgca actggcctgc
      661 gatgccctgc agatggcgct gtggcggcgt aagaggcccc ggaacgttat cgttcacacg
      721 gaccgtggag gccagtactg ttcagcagat tatcaggcgc aactgaagcg gcataatctg
      781 cgtgg
//
LOCUS       DAZGXZ010000406          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000406 DAZGXZ010000000
VERSION     DAZGXZ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.409"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            52..642
                     /locus_tag="ACHKJF_005645"
     CDS             52..642
                     /locus_tag="ACHKJF_005645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0952784.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
BASE COUNT          240 a          163 c          174 g          201 t
ORIGIN      
        1 ttttctgaga tgcattatga tatgaacacc aatttcgtat agagtctcac tatgtctcga
       61 atttttgctt actgtcggat atcaacgctg gatcagacca tcgaaaatca acgccgggaa
      121 atcgaaagtg caggttttaa aatcaaacct cagcaaataa tcgaagaaca cattagcggc
      181 tcagcagcaa ccagtgagcg tcctggtttt aaccggttgc ttgctcgcct gaaatgtggt
      241 gatcaattga ttgtgacaaa actggatcgc cttggttgta atgcaatgga tatcaggaaa
      301 acagtggaac aactgaccga aacaggtatc agagtgcatt gcttagcatt ggggggcatt
      361 gacctgacca gtccaacagg aaaaatgatg atgcacgtaa tttcagcagt cgctgaattt
      421 gaacgagacc ttttacttga acgcactcat tccgggatag taagagcccg cggcgcaggg
      481 aaacgttttg gtcgaccacc tgtgttaaat gaagaacaga aacaggtggt attcgaacga
      541 attaagtcag gtgtaagtat aagtgccatt gcccgggaat tcaaaacctc gcggcaaacc
      601 attttaagag ccaaagcaaa acttcagaca cctgacatat aaaaaataat ctcggtgtga
      661 gatgctttac gtcttccaag cccccttcct tgccgtaaat ggaaagatac atctaattat
      721 agaatttata tgttttaccc tacggcagtg ctggccattc aatatcctgt gcagttga
//
LOCUS       DAZGXZ010000407          771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000407 DAZGXZ010000000
VERSION     DAZGXZ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.410"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(115..701)
                     /locus_tag="ACHKJF_005646"
                     /pseudo
     CDS             complement(115..701)
                     /locus_tag="ACHKJF_005646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            681..>771
                     /locus_tag="ACHKJF_005647"
     CDS             681..>771
                     /locus_tag="ACHKJF_005647"
                     /inference="COORDINATES: protein motif:HMM:NF024169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /protein_id="HFP0952785.1"
                     /translation="MTGYWALSVHENTHKPGVKEQITEMAFNGA"
BASE COUNT          206 a          163 c          172 g          230 t
ORIGIN      
        1 aataaaggaa tagaatattt gcaagggtat cttttttcac cacctgtatc tggagatgtt
       61 tttattcata aatggatttt gtcaaccgaa aaaaggagca attagatgtg tgattcatag
      121 gcatcgtttc aattttctca gtccaagttc aagcttgtaa ttctgttcct gtatatgctc
      181 ttcattgggc gatagtttgc atgaacggca tcagagtatg gtgttttctc agcaagttca
      241 aagtagcgtt ccatcggagt tttaccgttg tgagcgctat gaggacgttc ccagttatag
      301 taatgctgcc attctgcctg cagttccttc aggtcatcag cagaaagatc tacggtagcg
      361 taaaactcag ctttgtctgt cttttgggaa cgttcaacct ttccgttaag atgaggcgac
      421 gctggcttat ttggacgaaa cttgatgccg tattctttta atctttcctg cactttaacg
      481 gcaaaaaata ctcttccccg gtctgtctga aagcgctgta tcgggaaaga catttcttca
      541 ataacacaat caataaaatc cagtgtgttt gccgccgtgc gacgagagta gagcctcagg
      601 actctgtagc gagtgcaatc atcaatagat gtgtactgat ataagccggg gccaatttta
      661 caggtatcca tctgaacccg atcaccggtt attgggcgct ctccgttcat gaaaatacgc
      721 ataagcccgg cgtcaaagag caaatcaccg aaatggcgtt caacggagca g
//
LOCUS       DAZGXZ010000408          765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000408 DAZGXZ010000000
VERSION     DAZGXZ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.411"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            151..226
                     /locus_tag="ACHKJF_005648"
     tRNA            151..226
                     /locus_tag="ACHKJF_005648"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:185..187,aa:Glu,seq:ttc)
     gene            411..>765
                     /locus_tag="ACHKJF_005649"
     rRNA            411..>765
                     /locus_tag="ACHKJF_005649"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          223 a          169 c          201 g          172 t
ORIGIN      
        1 agtgctcaca cagattgtct gatgaaaagt aaatagcaag gcgtttacgc gttgggagtg
       61 aggctgaaga gaataaggcc gttcgctttc tattaatgaa agctcaccct acacgaaaat
      121 atcacgcaac gcgtgataag caattttcgt gtccccttcg tctagaggcc caggacaccg
      181 ccctttcacg gcggtaacag gggttcgaat cccctagggg acgccacttg ctggtttgtg
      241 agtgaaagtc gccgacccca atatctcaaa acagactgtt aagtcttgtt tgagatattt
      301 gctctttaaa aatctggatc aagctgaaaa ttgaaacact gaacaacgaa agttgttcgt
      361 gagtctctca aattttcgca actctgaagt gaaacatctt cgggttgtga ggttaagcga
      421 ctaagcgtac acggtggatg ccctggcagt cagaggcgat gaaggacgtg ctaatctgcg
      481 ataagcgtcg gtaaggtgat atgaaccgtt ataaccggcg atttccgaat ggggaaaccc
      541 agtgtgtttc gacacactat cattaactga atccataggt taatgaggcg aaccggggga
      601 actgaaacat ctaagtaccc cgaggaaaag aaatcaaccg agattccccc agtagcggcg
      661 agcgaacggg gagcagccca gagcctgaat cagtgtgtgt gttagtggaa gcgtctggaa
      721 aggcgcgcga tacagggtga cagccccgta cacaaaaata cacat
//
LOCUS       DAZGXZ010000409          755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000409 DAZGXZ010000000
VERSION     DAZGXZ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.412"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(84..>755)
                     /locus_tag="ACHKJF_005650"
     rRNA            complement(84..>755)
                     /locus_tag="ACHKJF_005650"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          163 a          245 c          163 g          184 t
ORIGIN      
        1 ttatcagacc gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc
       61 cgccaaaaca ccttcggcgt tgtaaggtta agcctcacgg ttcattagta ccggttagct
      121 caacgcatcg ctgcgcttac acacccggcc tatcaacgtc gtcgtcttca acgttccttc
      181 aggactctca aggagtcagg gagaactcat ctcggggcaa gtttcgtgct tagatgcttt
      241 cagcacttat ctcttccgca tttagctacc gggcagtgcc attggcatga caacccgaac
      301 accagtgatg cgtccactcc ggtcctctcg tactaggagc agcccccctc agttctccag
      361 cgcccacggc agatagggac cgaactgtct cacgacgttc taaacccagc tcgcgtacca
      421 ctttaaatgg cgaacagcca tacccttggg acctacttca gccccaggat gtgatgagcc
      481 gacatcgagg tgccaaacac cgccgtcgat atgaactctt gggcggtatc agcctgttat
      541 ccccggagta ccttttatcc gttgagcgat ggcccttcca ttcagaacca ccggatcact
      601 atgacctgct ttcgcacctg ctcgcgccgt cacgctcgca gtcaagctgg cttatgccat
      661 tgcactaacc tcctgatgtc cgaccaggat tagccaacct tcgtgctcct ccgttactct
      721 ttaggaggag accgccccag tcaaactacc cacca
//
LOCUS       DAZGXZ010000410          746 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000410 DAZGXZ010000000
VERSION     DAZGXZ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.413"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>746
                     /locus_tag="ACHKJF_005651"
     CDS             <1..>746
                     /locus_tag="ACHKJF_005651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX toxin"
                     /protein_id="HFP0952786.1"
                     /translation="QSGTLSDDDRGGFIWLGGLAFGGGLAAMALAAGGGGGSKHRHEN
                     HEADTTAPSAPELTAGDNGSVSVELPGDANKGDTVEITFEDENGDEQKVTLEKGDNGW
                     TSSNPALIPDSQGDTATIAPDTVKDNSEVTAVAKDPAGNESTPVTVTSKTDGVADAPV
                     LTIPEVADSVANAAELKDGLQAEVKLPAGTVEGAVITLTVTHPDQSTENVTHNVTGDE
                     VTAGKVSMDIPEDAVVDGQNSVSVSLTQGS"
BASE COUNT          205 a          154 c          224 g          163 t
ORIGIN      
        1 caatccggga ccttgtcaga tgatgatcgt ggtggtttta tatggttggg cggtctggca
       61 tttggtgggg ggcttgctgc tatggctttg gctgcaggtg gtggtggcgg cagcaagcac
      121 agacatgaaa accatgaggc agatacaaca gcaccgtcag caccggaatt aacggccgga
      181 gataatggtt cagtttctgt tgaactgccg ggtgatgcaa ataaaggcga tactgttgaa
      241 atcacctttg aagatgaaaa tggtgatgaa cagaaagtca cactggagaa aggggataac
      301 ggctggacat ccagcaatcc tgcactgatc ccggacagcc agggtgatac agccacaatc
      361 gccccagaca ctgttaaaga taacagtgaa gtcacagcag ttgctaaaga tccggctggt
      421 aatgaaagta ctccggtcac tgtgacgtca aaaactgatg gtgtggcaga tgctccggtc
      481 ctgaccattc cagaagtggc tgatagtgtg gccaatgcgg ctgagctgaa agatggttta
      541 caggcagaag tcaagttgcc ggcaggaaca gtggaaggtg cggtcattac actgacagtg
      601 actcatccgg atcaaagtac cgaaaatgtg acacataacg tcaccggaga tgaggttact
      661 gccggtaagg tatccatgga tattcctgag gatgctgttg ttgacggtca gaacagtgtg
      721 agtgtcagcc tgacccaggg cagtaa
//
LOCUS       DAZGXZ010000411          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000411 DAZGXZ010000000
VERSION     DAZGXZ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.414"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..102
                     /locus_tag="ACHKJF_005652"
     CDS             <1..102
                     /locus_tag="ACHKJF_005652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0952787.1"
                     /translation="VRDFWRRQIDKRAAEKISASQSAGSKPEEPITP"
     gene            128..412
                     /locus_tag="ACHKJF_005653"
     CDS             128..412
                     /locus_tag="ACHKJF_005653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0952788.1"
                     /translation="MSEVKPLVTADAVKDALRSEDVRGALKQKVRQTFEAQIEADVDA
                     ILDTILGEQPGEQPEPEPQPEDITSENGDIQPESPVVDATEAQPEPGTML"
     gene            488..703
                     /locus_tag="ACHKJF_005654"
     CDS             488..703
                     /locus_tag="ACHKJF_005654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0952789.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGD"
BASE COUNT          206 a          174 c          220 g          141 t
ORIGIN      
        1 gtgcgcgatt tctggcgcag gcagattgat aaaagggccg cagagaaaat cagcgccagt
       61 cagtcagccg gaagcaaacc cgaagagccc attactcctt aacaacccct ttcaacgaga
      121 aaaccctatg tcagaagtaa aacccctggt cactgctgat gcagtgaaag acgcgctgcg
      181 ctcagaagat gtgcgtggag cactgaaaca gaaagtccgc cagacatttg aagcgcagat
      241 agaagcagat gtggatgcga ttctggatac gatactggga gagcagccag gcgaacagcc
      301 agagccggaa ccgcaaccgg aagatatcac ctcagaaaac ggcgatattc aaccggaatc
      361 accggtggtt gatgcgactg aagcacaacc cgaaccgggc accatgctgt aagggtgagt
      421 cagggccatc agtaaagtac tgctggcctt tttcatgttg tgagcttccg tgtgcgggag
      481 acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt
      541 gggaacgggc tactggttcc tgcagttgct ggacagggtt tccccgtctc agtgggcggc
      601 aataggcgtg ctggggagtc tgctgtttgg gctgctgaca tatctgacga acctgtattt
      661 caagattaaa gaagaccggc gtaaggcggc acggggagat taggtgatga accatgaaga
      721 aatgaatcag cgcttcagtc g
//
LOCUS       DAZGXZ010000412          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000412 DAZGXZ010000000
VERSION     DAZGXZ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.415"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..486)
                     /locus_tag="ACHKJF_005655"
     CDS             complement(<1..486)
                     /locus_tag="ACHKJF_005655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP0952790.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PL"
     gene            complement(502..>726)
                     /locus_tag="ACHKJF_005656"
     CDS             complement(502..>726)
                     /locus_tag="ACHKJF_005656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP0952791.1"
                     /translation="NSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVS
                     ILLALISVATCWLLWKYRAILTNLVRLKIK"
BASE COUNT          246 a          124 c          126 g          230 t
ORIGIN      
        1 taacggcaat tgtatgtgat caacaaaatc aggaaataat aaattaaatc gttgctgcat
       61 tgccaatggt tgttgcctta tatagggtga caacgttgaa aaaccacatg tgatgatatt
      121 gtgatagcta gcgatgccgg gtttgtatcg tgctttttca tttattttca ccggtatagc
      181 tgataaataa tcccttagca cagcaggaac cttataaaaa aatccgcgtt ctgcaaacct
      241 ttctcgataa aagtgataac cactgaataa acagaacaat ggaaataaat tccaattgtt
      301 tatgtagtaa ataagtgaac tattcagatt aaaatgttca actgaaagat tgtactgcag
      361 aacaatcatt tcattggtaa tagatctaag caccggattg ttaataggtg tattattcaa
      421 gcttactcgt tgaggatgaa tataactaat tggatcatat ataatctttc ttagggcaag
      481 attcataacg ctctcatatg tctacttaat tttcaacctg actaaattag ttagaatggc
      541 cctatacttc cataacagcc agcaagtcgc tacggatatt aatgcaagta agatagaaac
      601 cggcatagcc ttatcataag caaaaacagg ttcgctaatt tcatatgttg gtgcttgctc
      661 aataatgtct cttcgttttg acaatacaac agaaatattt tcatattgta cgcttgcaga
      721 gctatt
//
LOCUS       DAZGXZ010000413          725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000413 DAZGXZ010000000
VERSION     DAZGXZ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.416"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(255..407)
                     /locus_tag="ACHKJF_005657"
     CDS             complement(255..407)
                     /locus_tag="ACHKJF_005657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952792.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            425..616
                     /locus_tag="ACHKJF_005658"
     CDS             425..616
                     /locus_tag="ACHKJF_005658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP0952793.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          214 a          168 c          121 g          222 t
ORIGIN      
        1 ttaaagaagg caggaacagc ggaagccccc accatttatc acgtattttt atataagttg
       61 cattcagttt catagatgct cagcagaatc ccccacatcc tgaaggaggt gtattcagac
      121 aggcatccca cctgacttcg aatgatgatt attcatcact atagagagca ttgattctaa
      181 gtgtcatatg aaagttccaa ttgatatata tcaaacaaaa taaccctgat taatgaatta
      241 ttacgtttat catgttaatt catcattatt acatcatcat tgtaaataat taaattaact
      301 tccataacat taaaatatgt atccactgac gcttttttac ataacgaaga attgaccatt
      361 ttgtcctgtt gtgccttaat gtaagtaccg tccacagcgt gggacatact tcaaggaacc
      421 ttttgtgagt caggcaacca gtatgcgaaa acgacaccga tttaacagtc gcatgacccg
      481 tatcgtactg ctcatcagct ttatcttctt ctttggccgt tttatctact cgtccgtcgg
      541 tgcctggcag caccatcaga gcaaaaaaga agctcagcaa tccacactct ccgtcgaatc
      601 accggtacaa cgttagcggt taccttctcc actttcacag aacctaacgg cacttcgctg
      661 tcggatgctt ttgctctttg gaattatcaa agcggcagat attctttcat cttaaatttt
      721 acgtc
//
LOCUS       DAZGXZ010000414          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000414 DAZGXZ010000000
VERSION     DAZGXZ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.417"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..613
                     /locus_tag="ACHKJF_005659"
     CDS             <1..613
                     /locus_tag="ACHKJF_005659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952794.1"
                     /translation="ALLQYCSSKMDLPSNNDTYHEMYHALNMNLSSKNFEDGHISTGT
                     IFFDTESNKWYLCVSAACDLVPTQGNDPHHVRLSPHRLIKVLELFNASQSKALPFAEH
                     SKYIYVMHKNQRKYLSIFEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMDGN
                     VQNVPVRLKLKSQLRTGYAERYQAIASQYSSRIGVDYVSMMLP"
BASE COUNT          216 a          117 c          134 g          252 t
ORIGIN      
        1 tgcattgctc caatattgct cttcaaaaat ggatctgcca tcaaataatg atacttatca
       61 cgaaatgtat catgctttaa atatgaattt gtcttcaaag aattttgaag atggtcatat
      121 ttctactggc actattttct ttgatacaga gtcgaacaaa tggtatttat gtgtatctgc
      181 ggcatgtgat ttggttccta ctcagggtaa cgaccctcac catgtaagat taagtccgca
      241 caggctcatt aaagttctgg agctttttaa cgccagtcag agtaaagcat tgccatttgc
      301 tgaacattcg aaatatatat atgtaatgca taaaaatcaa agaaaatatc tctctatttt
      361 cgaaggggat aaaacgcttc ctgttgttga ttatatggtg gtgttgaatc atggaacaac
      421 agttgatggc gaagaaaaaa atattatttc tgccgtgttt ttaagtaata tggatggcaa
      481 cgtgcaaaat gttcctgtcc gactcaaact taaatctcaa ctgagaactg gttatgcaga
      541 aagatatcag gctatagcgt ctcagtatag ctcaaggatt ggtgtggatt atgtatcaat
      601 gatgctacca taattattat attttaggcg tggtgatttt tttcgccatg cctattttat
      661 atttatcatc acaatgatgt tgtttatttt tgtttaagtc ttctaagctt catgcattc
//
LOCUS       DAZGXZ010000415          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000415 DAZGXZ010000000
VERSION     DAZGXZ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.418"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            2..220
                     /gene="ygeI"
                     /locus_tag="ACHKJF_005660"
     CDS             2..220
                     /gene="ygeI"
                     /locus_tag="ACHKJF_005660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFP0952795.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDKDQIQAF
                     IVSTWMASFQNDMYSEDNPISPYHKIEW"
     gene            305..>715
                     /locus_tag="ACHKJF_005661"
     CDS             305..>715
                     /locus_tag="ACHKJF_005661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0952796.1"
                     /translation="MRSPKVKFLTIFTLSILITKMSFASSACFNEAGTMFRIEPNLIK
                     AIALVESNLKKDSIGKNRDKKNNIKSFDYGLMQINQMHIPMLKKRGIIKDERDLLDNP
                     CLNIKIGTEILYKHFSRCGMTWQCLGTYNAGFAMD"
BASE COUNT          263 a          116 c          122 g          214 t
ORIGIN      
        1 tatgactaat ccaatcggta tcaataattt atctcaaagt tcaaatatag cgaatgcaac
       61 gggtgatgaa gtcgtttcat tagataaaca catcaacacc agtgctactg ataaagatca
      121 aatacaagca tttatagttt caacatggat ggcgtctttt caaaatgata tgtattcaga
      181 agataatcct atctcacctt accataaaat tgaatggtag acgattgacc aaataaatta
      241 ttaacgagat actgtttata atagtatctc gttcctttta tacaatcctg actcataaag
      301 atatatgcgc tcaccaaaag ttaaattctt aaccatcttc acgctttcta tattaattac
      361 aaaaatgtct ttcgccagta gcgcttgctt taatgaagca ggaacaatgt tcagaatcga
      421 accaaatttg attaaagcta tcgctcttgt tgaaagcaat ctaaaaaagg atagtatagg
      481 gaagaacaga gataagaaaa acaatataaa gagttttgat tatggtttaa tgcagataaa
      541 ccagatgcac attcccatgt taaaaaaacg tggaataata aaagatgaac gagatttgtt
      601 agataatccc tgcctgaaca ttaaaatagg aaccgagatt ttatataagc atttttcccg
      661 ttgtggaatg acgtggcaat gcctggggac ttataatgcg ggttttgcaa tggat
//
LOCUS       DAZGXZ010000416          708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000416 DAZGXZ010000000
VERSION     DAZGXZ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.419"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..222
                     /locus_tag="ACHKJF_005662"
                     /pseudo
     CDS             1..222
                     /locus_tag="ACHKJF_005662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            261..>708
                     /locus_tag="ACHKJF_005663"
     CDS             261..>708
                     /locus_tag="ACHKJF_005663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0952797.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQG"
BASE COUNT          253 a          151 c          115 g          189 t
ORIGIN      
        1 gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta tggcaaggtt
       61 accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac ttatgcccgt
      121 ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg tcactgggaa
      181 tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaagaa tctcttattg
      241 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
      301 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      361 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      421 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
      481 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      541 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acctctatca
      601 atcttgataa taaccaactc acacattttg atgcaaccaa ctacgataga ctcgtaaagc
      661 taagtctgaa tagtaatgct cttgagtcaa taaattttcc tcaaggca
//
LOCUS       DAZGXZ010000417          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000417 DAZGXZ010000000
VERSION     DAZGXZ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.420"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(441..>691)
                     /locus_tag="ACHKJF_005664"
     CDS             complement(441..>691)
                     /locus_tag="ACHKJF_005664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952798.1"
                     /translation="DENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
BASE COUNT          154 a          217 c          205 g          115 t
ORIGIN      
        1 agagtgacag atttcgcata ctgcatggga tttcctccgt tgttcacata ttccgcgaca
       61 ccgggcggtg ccgtgcctcc atctttgccg ggcctgcagg gttgcaaggg cgcaagggaa
      121 gccgcagcct gacgcacacg gctgacggga gttggagggt gagaccgcga aatctgagcg
      181 ggtgaacgac cggaaaatca cggcagacgg gaaggaggga ccgggtgacc gcagcggttc
      241 accttacctt gcaacccgcc cgcagaagcc cggctacgat ggaggcacgg cacagcccgc
      301 acacgacgct gaggccacat aactgaaaac ccacaaggcc atgccccgca tggccgggta
      361 aagcggagcc tgcggcgaca tccttttgct ctgtccgggg acaggcagga aaaggcggag
      421 atttttcccc gccagcgtca tcatttccgg atttttgcca gggcatccgc catgctgcga
      481 atttcggccc gtgcttcccg gaccacctcc cgcagaacac cacggtcaaa ccaccggcgg
      541 ggcggttcat ccggtatacg cagcacatcg caggccagcc cactggacag gatatgctca
      601 atacaatcgc tgaccagata catttccacc gccgaaaaac gtgcgccccg gttcaggatg
      661 gcatcgagca ccacgggcag attttcgtca t
//
LOCUS       DAZGXZ010000418          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000418 DAZGXZ010000000
VERSION     DAZGXZ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.421"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..666
                     /locus_tag="ACHKJF_005665"
     CDS             <1..666
                     /locus_tag="ACHKJF_005665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0952799.1"
                     /translation="LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYGY
                     QLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQ
                     SICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSH
                     GEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVH
                     GNM"
BASE COUNT          235 a          119 c          156 g          176 t
ORIGIN      
        1 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
       61 gacccgctgc aggggcgata tatcactcag gatccgattg gactgaaggg gggatggaac
      121 ctgtatggat atcaattgaa tccgatatca gacatcgacc ccctgggttt atctatgtgg
      181 gaggatgcaa aatcgggggc atgtactaat ggtctttgcg gcacactatc cgctatgata
      241 ggtccagata aatttgattc tatagatagc accgcatatg acgccttaaa taaaataaat
      301 agccaatcta tttgcgaaga taaagagttc gctggtttaa tatgtaagga taatagtggc
      361 agatatttct caacagcacc taaccgagga gaaagaaaag gatcatatcc attcaatagc
      421 ccttgcccta atggtactga gaaagtatca gcttatcata ctcatggtgc agatagtcat
      481 ggagaatatt gggacgaaat attttcaggt aaagatgaga aaatagttaa aagtaaagat
      541 aacaatatca agtcatttta tttaggtacg cccagtggta attttaaagc aatagataac
      601 cacggaaagg aaataacaaa cagaaaagga ttacctaatg tctgcagagt tcatggtaat
      661 atgtaaaaaa aatattgttt aggaac
//
LOCUS       DAZGXZ010000419          682 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000419 DAZGXZ010000000
VERSION     DAZGXZ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.422"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>682
                     /locus_tag="ACHKJF_005666"
     CDS             <1..>682
                     /locus_tag="ACHKJF_005666"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0952800.1"
                     /translation="AMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTP
                     FYPRSPYAVAKLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANI
                     AQGLESCLYLGNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMA
                     AEQVGIKLRFEGVGVNEKGIVVSVTGKDAPAVKSGDIIVKVDPRYFRPAEVETLLGDP
                     TKAHEKLGW"
BASE COUNT          166 a          188 c          183 g          145 t
ORIGIN      
        1 gcgatgggta cgctgcgcct gctggaggcg atccgcttcc tcggtctgga aaagaaaacc
       61 cgtttctatc aggcttccac ctctgaactg tatggtctgg tgcaggaaat tccgcagaaa
      121 gaaaccacgc cgttctaccc gcgatctccg tatgcggtcg ccaaactgta cgcctactgg
      181 atcaccgtta actaccgcga atcctacggc atgtacgcct gcaacggtat tctcttcaac
      241 catgaatccc cgcgccgtgg cgaaaccttc gttacccgca aaataacccg cgcaatcgcc
      301 aacatcgccc aggggctgga gtcgtgcctg tacctcggca atatggattc cctgcgtgac
      361 tggggccacg ccaaagacta cgtaaaaatg cagtggatga tgctgcaaca ggaacagccg
      421 gaagatttcg ttatcgcgac cggcgttcag tactccgtgc gtcagttcgt ggaaatggca
      481 gcagagcaag tcggaatcaa attgcgcttt gaaggtgtcg gcgtaaatga gaagggaatc
      541 gttgtttctg ttactggtaa ggatgcccca gccgttaaat ccggagatat aattgttaag
      601 gttgacccgc gctatttccg tccggcagaa gtagaaacgc tgctcggcga cccgaccaaa
      661 gcgcacgaaa aactgggctg ga
//
LOCUS       DAZGXZ010000420          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000420 DAZGXZ010000000
VERSION     DAZGXZ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.423"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>671)
                     /locus_tag="ACHKJF_005667"
     CDS             complement(<1..>671)
                     /locus_tag="ACHKJF_005667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0952801.1"
                     /translation="TPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQ
                     RYHYDL"
BASE COUNT          101 a          226 c          188 g          156 t
ORIGIN      
        1 aggtcataat gataacgttg cgtccgcccg tcaaagccgc cctgctgtac caaccggtcc
       61 agcgcatcgt agctgaagtc gctgtggctg ccgttctcgt tagtcagact gatgacacgt
      121 ccggcagcat cgtactccat actgcgcgtc agcccgccct gcgtggtgct gaccgccttt
      181 ccccacgcat cgtactgtgt ctcgctccgg ttgccgtccg gggtgataac ggcagtcagg
      241 tcgcctgcgg cgttgtactc atactgcgtt tcacggccct gtgcgtcttt caccgaggtt
      301 aaccggccac ggttgtcata gtggcggtaa aggctgatac cttcctcgcg gtgaaccgcc
      361 gtcatctggc cgaagcggtc gtattcatag cgggtctggt agcccgagca gtcagtgaac
      421 gccagcaact gcccgtagcg gctccacgtc atctgccggg tgctgcccgt cgcatccgtt
      481 gtcgtcgccg gtaactcact gtgcgcgtca tcgtagcggt agcgtactgt ctccccgctg
      541 cgcgatgtct ccgataccag cctgcccggt tcatcatatt cccggcggct ctccagcccg
      601 tccggggaca ccaccgccgt cagctggttc ccgtcgttat agtaaaattt cgtctcccgc
      661 ccgtccggtg t
//
LOCUS       DAZGXZ010000421          671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000421 DAZGXZ010000000
VERSION     DAZGXZ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.424"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          170 a          157 c          130 g          214 t
ORIGIN      
        1 aggtggtaat cgcatagccg tgcgtgcgcg gttatacgat tacacatcct gtcccggaat
       61 caggattgtt tttcgcgcac aactgcggca aattcatccc gttgcggttg caacgatggg
      121 tatatcaaaa ctacgaaaat tattcaggca tttgctaccc cccttgccct ggcctccacc
      181 agtcacgggg gatttttttg ttgtctctca tgacgaacta cggcaaattt ctgacgttgc
      241 aaactgcaac acataaaaca cacacaaaac tcacaattgc catgtcctca ttgacacccc
      301 cttgccatat agggggattt tttcgcctgt cctgattgtc ttttgccgat ggttacggca
      361 gattggaaaa gagcgcattt gtggtgctct ctccaaaata atatttctca ttactcaaac
      421 tctgatggct tccccctgta atccaggggg attttttaat ttatcgctat gcttcgtaat
      481 tcattcagaa tacagacaat atggttaatc agtttttcat catttttgcg gtgtttgccc
      541 tgtcctgcat cagaacgcta aaaaaacagg aatctcttta tgacaaatcc tccatttcag
      601 gattttttat gtagcgtcta ctacatgcga tccgttggtg agctgtttat tatgcattag
      661 ttatataata a
//
LOCUS       DAZGXZ010000422          669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000422 DAZGXZ010000000
VERSION     DAZGXZ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.425"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..291
                     /locus_tag="ACHKJF_005668"
     CDS             <1..291
                     /locus_tag="ACHKJF_005668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952802.1"
                     /translation="VSVQELKASFCSVKTHSTNNISADIILTRTPHITEFSSDSITAQ
                     IKKNGINIQTGRMVSQKHLIITHDQEGKHLTTWYQQISLRVHSYKKHTEEVR"
BASE COUNT          231 a          162 c          106 g          170 t
ORIGIN      
        1 gtttcagtac aggaattaaa agccagcttt tgctcagtaa aaacacactc aactaataat
       61 atctcagcag atattatcct gaccaggaca cctcacatta ctgaattctc ttccgacagt
      121 atcactgcac aaataaaaaa gaacgggata aatattcaga ccggaagaat ggtgtcacaa
      181 aagcatttaa tcattacgca tgaccaggaa gggaaacact taaccacatg gtatcaacag
      241 atatcattac gtgtgcactc ttataaaaaa cacacagaag aagtaagata attataaatg
      301 taaatttaat atctcccact gacatctccc ccacaataca ctgaaaaata ctcacagcag
      361 taaacacaga cacacctgat aatcatgcag ccgataaata ttttcccctt tacggagaaa
      421 tcagcctgct gtttaacaat aacggcgtat tttcatggaa ggagatcacc cagtaacgga
      481 taagtcctga tctccttcat gttaatacgc aacaatccca tcgattcaaa acaatttcag
      541 acagatatta tctccgtgaa tcccccgcca cctttccggt gcgcggggtt ttgtcttttt
      601 tcaccgggaa tacatgtatg aatccgtctg atgccattga ggcaattgaa aaaccgctct
      661 cctccctgc
//
LOCUS       DAZGXZ010000423          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000423 DAZGXZ010000000
VERSION     DAZGXZ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.426"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            1..660
                     /locus_tag="ACHKJF_005669"
                     /pseudo
     CDS             1..660
                     /locus_tag="ACHKJF_005669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          223 a           96 c          120 g          221 t
ORIGIN      
        1 actacgtaat ttcaatgggg tgccaggttt aaacagagat aactttattt atatcagcaa
       61 tattttttca aatataaaac aacggaacga aaaaatcatg caataaattt gtttcgtgaa
      121 gtctcaatca gtaatgatac tataagtgta aaattctaca gaaatgaaaa aaattgaatg
      181 cgcttgcaat tttctgatgg ataaagatgc gcaggggtat atcgacctgt ctgatttgga
      241 tttaacaagt tgtcatttta aaggtgacgt tatatcgaag gtgtcttttt tatcatcaaa
      301 tctacaacat gtaacattcg aatgtaaaga aattggggat tgcaatttta ctactgcaat
      361 agttgataat gtcatattta gatgtcgacg tttacacaat gtgattttta tcaaagcgag
      421 tggtgaatgt gtcgatttca gcaaaaatat tcttgataca gttgacttct cgcagagtca
      481 acttactcag agtaattttc gcgaatgtca gattagaaat tcaaacttcg ataattgtta
      541 tctttacgct tcgcacttca ccagagcaga gtttctgtct gccaaagaaa tatcatttat
      601 taaatcgaat atgacagctg ttatgtttga tcatgtgcga atatcgacag ggaattttaa
//
LOCUS       DAZGXZ010000424          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000424 DAZGXZ010000000
VERSION     DAZGXZ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.427"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..615
                     /locus_tag="ACHKJF_005670"
     CDS             <1..615
                     /locus_tag="ACHKJF_005670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0952803.1"
                     /translation="LPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTY
                     PLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYLPMN
                     PISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDL
                     TNSYMNGMEKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT          240 a           99 c          147 g          174 t
ORIGIN      
        1 ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca ccgctattat
       61 gacccgctgc aggggcggta tatcactcag gatccgattg ggctgaaggg gggatggaac
      121 ctgtatacat atccgctgag cccggtgaat agcatggatc cattaggatt atatgaattt
      181 aaatcaaaaa atatagatga tattggaata tttgcattgg caatgtgtaa tggagaatca
      241 attaacgaga ataaagaata tggtggacta atatgtaaga agcaaggtga atatttaccc
      301 atgaatccga taagttcaaa tgataatgat agtgtagact tgcgaaatat aaaatgccct
      361 gaaggttcag agagagtagg cgattatcac actcacggtt tttactctga cgataaagga
      421 aataaagtaa caaaagaaaa tgatgtttat gatagtctaa atttttcaag caaagattta
      481 acgaattctt atatgaatgg aatggaaaaa aaagaataca gtagttactt gggaacacca
      541 aataacacct atctaaaata taatcccaaa gctaaaggga atggagttac aattatcagg
      601 caagggagta attaaaatga aggcaataca atgttgttat ttccttgctt tagtctccag
//
LOCUS       DAZGXZ010000425          648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000425 DAZGXZ010000000
VERSION     DAZGXZ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.428"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            86..355
                     /locus_tag="ACHKJF_005671"
     CDS             86..355
                     /locus_tag="ACHKJF_005671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0952804.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGAPTVPEPEGIADDSAVSDGVVSQPDGGSEPQPDGEMMM"
     gene            431..646
                     /locus_tag="ACHKJF_005672"
     CDS             431..646
                     /locus_tag="ACHKJF_005672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0952805.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          156 a          149 c          200 g          143 t
ORIGIN      
        1 gccagtcagt cagccggaac aaaacccgaa gagcctctca ttccgtagca actttcttaa
       61 caacaccttt caacgagaaa ataccatgtc agaaataaaa tcgctggtca ctgctgaagc
      121 agtgaaggaa gtcctgcgct ctgaagaagt cagaagcgca ctgaaacagc aactccggca
      181 gaatcttgag gcgcgtcttg atgcagaagt ggatgccatt ctggatgaac tgctgggcgc
      241 accgactgtt ccggagccgg aaggcattgc ggatgacagt gctgtttcag atggtgtcgt
      301 gtctcagcct gatggtggca gtgagcctca gcctgacggc gaaatgatga tgtaaccatg
      361 cgcaggggct gtcggtgtga gctgatgccc ctcttgttgt tgtgagcttc cggattgcgg
      421 gagacggggt atgtaccaga tggaaaaaat aacaacaggt gtgtcataca ccacgtcagc
      481 ggtgggaacg ggctactggt tcctgcagtt gctggacagg gtttccccgt ctcagtgggc
      541 ggcaataggc gtgctgggga gtctgctgtt tggtctgttg acgtacctga cgaacctgta
      601 tttcaaaatc agagaggacc gtcgtaaggc ggcacgggga gagtaggt
//
LOCUS       DAZGXZ010000426          640 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000426 DAZGXZ010000000
VERSION     DAZGXZ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.429"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..575)
                     /locus_tag="ACHKJF_005673"
     CDS             complement(<1..575)
                     /locus_tag="ACHKJF_005673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001379928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0952806.1"
                     /translation="MLKDGTGKPVPGCTIELKARRTTETVIVTTVAYGQPGETGSYSM
                     DVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKALAELEAV
                     AAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERGPKGERGE
                     TGPQGLQGVKGERGEKGEKGEPGGPDATTAQ"
BASE COUNT           94 a          251 c          129 g          166 t
ORIGIN      
        1 ttctgtgccg tcgtcgcatc cggccctccc ggctcacctt tttcgccctt ctctccccgc
       61 tcacctttca caccctgaag cccctgtggt cctgtctcac cacgctcacc ctttggcccc
      121 cgctcgccgg tatcgccttt tggcccggga ataccctgtg gccccgtgtc tcccttatcc
      181 cccttcggtc ccctcgcatt ctctgcccgt ttcttcgctt cctctgcact cgccgctgac
      241 gcttccgcac gtttcaggat ttccgcggcc accgcttcca gctctgcaag ggctttcggg
      301 taatactgtg catcctccag gtccatcaga aatttattca gcgttcccgg tgccgaatcc
      361 gccttcacca gaatgtcacc cacatatgac ggcgcgtacc cttccgtgtt cagcgtcacc
      421 cggtacaacc ccggctcaac atccatactg taactgccgg tttcccccgg ctgaccatac
      481 gccaccgtgg tgacaatcac cgtctccgtt gtgcggcgcg ctttcagctc tatcgtgcat
      541 cccggtaccg gttttcccgt accatccttc agcacacccg atattctgac tgtcatggat
      601 ttcccccata aaaaaaaccg cagtaccggt ttcccggcct
//
LOCUS       DAZGXZ010000427          635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000427 DAZGXZ010000000
VERSION     DAZGXZ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.430"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            105..>635
                     /locus_tag="ACHKJF_005674"
     CDS             105..>635
                     /locus_tag="ACHKJF_005674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFP0952807.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILENNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGS"
BASE COUNT          223 a           84 c          121 g          207 t
ORIGIN      
        1 atacatatct tatattcaaa aagaaaatta aaaaagtaat cagggatgta aaagtcaaac
       61 taggattgaa gagcaaataa tgattatgat gaagggtaat taaattggat tttaaacatc
      121 ttactcaatt taaagatata attgaactgg acaagcgccc cgttaaactt gatgaacggg
      181 aaacgtttaa tgtctcatgg ggtattgatg agaactacca ggttggggct gcgatttcaa
      241 ttgcttcaat tcttgaaaat aataaacaaa acaaatttac ctttcacata atcgctgatt
      301 acttagacaa agagtatatt gaattattat cacaattagc aacgaagtat caaacagtaa
      361 ttaaattata tcatattgat tctgagccat tgaaggcgct acctcaatca aatatctggc
      421 cagtatctat ttattatcgt ttgctttcat ttgattattt ttctgcgcga ttggattcat
      481 tattatatct tgatgctgat atcgtctgta agggttcatt gaacgagtta atagcattag
      541 agtttaaaga tgaatatggg gcagtggtaa ttgatgtaga tgctatgcaa agtaaaagcg
      601 ctgagcgttt gtgtaatgag gattttaacg gtagc
//
LOCUS       DAZGXZ010000428          634 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000428 DAZGXZ010000000
VERSION     DAZGXZ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.431"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            246..>634
                     /locus_tag="ACHKJF_005675"
     CDS             246..>634
                     /locus_tag="ACHKJF_005675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0952808.1"
                     /translation="MLKNLLNTEVVQVVEQAKDWREAVAISCRPLIENGSIEPRYVDA
                     IYRSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQA"
BASE COUNT          176 a          142 c          143 g          173 t
ORIGIN      
        1 agctgttttc attactttta ttttaccatt actctttcca gtttttgccc acggtagcaa
       61 atatcctgtt gcattgtata tgcaggaacc gaatatagcc aaagcatggc ggcagagaaa
      121 tgatagcgct atcacaaaga tcggtagcgt tatcagatag cgctatcaaa tgcgacaatc
      181 atcacattaa ttaatgcgta aaatatctac tctcctgtta attatcatcg ggagttatca
      241 gtcgcatgtt aaaaaatcta ttaaatacag aggtcgttca ggtcgtggaa caggccaaag
      301 actggcggga agccgtggcg atttcatgcc ggccgttgat tgaaaacggt tctatcgaac
      361 cgcgttatgt tgatgcgatc tatcgctcgc acgacactat cggtccgtac tatgtggttg
      421 gccctggtat tgcgatgcct catgcgcgcc cggaagaggg agctaacaag ctgtcattag
      481 cactgaccct gatcccttca ggtgtgaatt ttgatgccga tgaaaacgat ccagtgaaat
      541 tactgattgt gctggctgca accgacagta ccagccacat tgaagcgatt tctcaactcg
      601 ccaagttatt cgataacgaa aaagacattc aggc
//
LOCUS       DAZGXZ010000429          624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000429 DAZGXZ010000000
VERSION     DAZGXZ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.432"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..531
                     /locus_tag="ACHKJF_005676"
     CDS             <1..531
                     /locus_tag="ACHKJF_005676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0952809.1"
                     /translation="LLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDLT
                     VNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDLL
                     KNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKDA
                     TPGNVYAQADYKIAYE"
BASE COUNT          195 a          134 c          139 g          156 t
ORIGIN      
        1 cttcttgttg ctggcgtagc aatgattctt tccaataacg tatttgctga cgaaggtcac
       61 ggcattgtta aatttaaagg tgaagttatc tctgctccat gttcaattaa accaggtgac
      121 gaagacttaa ctgtaaatct gggtgaagta gcagataccg ttctgaaaag cgaccagaaa
      181 tctttggctg agcctttcac tattcacctg caagactgca tgttgagcca gggtggaacc
      241 acttattcaa aagcgaaggt tactttcacc acagcgaata ctatgacggg tcagactgac
      301 ctgctgaaaa acaccaaaga aactgaaatc ggtggtgcca caggcgttgg cgtacgtatt
      361 ctggatagcc agagcggcga agtgacttta ggtactccgg tagtaattac attcaacaat
      421 actaacagct atcaggaact gaacttcaaa gctcgtatgg aatccccaag caaagatgct
      481 accccgggca acgtttacgc tcaggctgac tataagattg cttacgagta atcacattag
      541 gccgggttcg cccggccaca tcattaacgg attaatgata agtggaacag atgtataaaa
      601 aattaaaatt aaccacaata agcg
//
LOCUS       DAZGXZ010000430          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000430 DAZGXZ010000000
VERSION     DAZGXZ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.433"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>621
                     /locus_tag="ACHKJF_005677"
     CDS             <1..>621
                     /locus_tag="ACHKJF_005677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0952810.1"
                     /translation="TLWHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSHTSEGHRV
                     SVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLT
                     YGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQH
                     LNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAP"
BASE COUNT          139 a          189 c          215 g           78 t
ORIGIN      
        1 tcaccctctg gcactacgat gcgtcggacc gcatcacgca ccggacggtg aacggcgacc
       61 cggcagagca gtggcagtat gatgagcacg ggtggttaac caccctcagc cataccagtg
      121 aaggccaccg cgtgtcggtc cactacgggt acgacgataa gggccgcctg acgggcgaac
      181 ggcagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acgaaacacg
      241 cctacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
      301 ggctgaccta cggcagcggt tacctggcgg gcatgaagct gggcgggacg ccgctggtcg
      361 agtatacgcg ggacaggctg caccgtgaga cggtgcgcag cttcggcagc agggcaggca
      421 gtaatgccgc atatgaactg accagcacat acacccccgc aggccagtta cagagccagc
      481 acctgaacag cctggtgtat gaccgtgact acgggtggaa tgacaacggc gacctggtgc
      541 gcatcagcgg cccgcgacag acgcgggaat acggctacag tgccacgggc aggctggaga
      601 gcgtgcgcac cctcgcaccg g
//
LOCUS       DAZGXZ010000431          621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000431 DAZGXZ010000000
VERSION     DAZGXZ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.434"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>621
                     /locus_tag="ACHKJF_005678"
     CDS             <1..>621
                     /locus_tag="ACHKJF_005678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0952811.1"
                     /translation="TLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRV
                     AVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLT
                     YGSGYLAGMKLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQH
                     LNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAP"
BASE COUNT          144 a          192 c          203 g           82 t
ORIGIN      
        1 tcaccctctg gtactacgat gaatcggacc gtatcactca ccgcacggtg aacggcgaac
       61 cggcagagca gtggcagtat gatgaccacg gctggctgac agacatcagc cacctgagcg
      121 aaggccaccg tgtcgctgtc cactacggct acgacgataa gggccgcctg accggcgaac
      181 ggcagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acaacacacg
      241 cctacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
      301 ggctgaccta cggcagcggt tatctggcgg gcatgaagct tggcgacacg ccgctgctgg
      361 agtacacgcg cgaccgcatg caccgggaga cggtgcgcag ctttggcagc atggcaggca
      421 gtaatgccgc atataaactg accagcacat acacccccgc aggccagtta cagagccagc
      481 acctgaacag cctggtgtat gaccgtgact acgggtggaa tgacaacggc gacctggtgc
      541 gcatcagcgg cccgcgacag acgcgggaat acggctacag cgccacgggc aggctggaga
      601 gcgtgcgtac cctcgcaccg g
//
LOCUS       DAZGXZ010000432          611 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000432 DAZGXZ010000000
VERSION     DAZGXZ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.435"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>611)
                     /locus_tag="ACHKJF_005679"
     CDS             complement(<1..>611)
                     /locus_tag="ACHKJF_005679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S6"
                     /protein_id="HFP0952812.1"
                     /translation="GGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDW
                     ESRTFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPV
                     KGTSAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGNSRL
                     TSSALTVSDGGRLHVKGGLETDGGVTLDRGTLLVDGGSVRNDV"
BASE COUNT          138 a          208 c          129 g          136 t
ORIGIN      
        1 tatacatcat tcctgacact tcccccgtcc accaaaaggg ttcctctgtc cagtgtcaca
       61 ccaccatctg tctccagtcc ccccttcaca tgtaaacgcc cgccgtcact gacagtaagt
      121 gcagaagagg tcaggcggga attcccttcc agacgcacat tctctgaact cacagctatc
      181 cggctgtccg atgccgttat actgccagta aaatggtcgc ggaccgtcag ggcagaatga
      241 cgcatattca cattcccgcg aaatgtactg ctaccggatg caccgcccac ggcagacgtc
      301 cccttaaccg gcgcactgct gctccccgtg ccgtccttca tatccagata tagcgagtca
      361 cttcccagca ccatggtggc attatcagca tcaatatctc ccgtcagtgt cgcattgcgg
      421 gaaaggctga atgccgcccc cttcagtttc agttgaccga tgctgaacgt tctgctctcc
      481 cagtccttct gagcaaatga taccggctgc gtcagaacag aattatcacc ctgctttcgc
      541 aatgcagtcg caaccgcctc actgctgaca gcatgaacaa ccggatgccc ctggatatac
      601 agtcccccgc c
//
LOCUS       DAZGXZ010000433          606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000433 DAZGXZ010000000
VERSION     DAZGXZ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.436"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            167..427
                     /locus_tag="ACHKJF_005680"
     CDS             167..427
                     /locus_tag="ACHKJF_005680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0952813.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
BASE COUNT          192 a          118 c          142 g          154 t
ORIGIN      
        1 tttcctgttt ttcagccatt tcgcaacaaa aatcacatta aacaacagta atcaccgttg
       61 atttgtgcgc caacacagat cttcgtcaca attctcaagt cgctgatttc aaaaaactgt
      121 agtatcctct gcgaaacgat ccctgtttga gaattgagga ggcgagatgt cgcagacaga
      181 aaatgcagtg acttcctcat tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac
      241 tgactctgag aaacaaatgg ccgctgttgc aagaaaacgt cttacacaca aagagataaa
      301 agtttttgtc aaaaatcctc tgaaagatct catggttgag tactgcgaga gagaggggat
      361 aacacaggct cagttcgttg agaaaatcat caaagatgaa ctgcagagac tggatatact
      421 aaagtaaaga ctttactttg tggcgtagca tgctagatta ctgatcgttt aaggaatttt
      481 atggcgggcc acgccgtaag gtggcaggga actggttctg atgtggatgt acaggagcca
      541 gaaaagcaaa aaccccgata atcttcttta actttggcga gtgagaaaga ttaccggggc
      601 catcta
//
LOCUS       DAZGXZ010000434          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000434 DAZGXZ010000000
VERSION     DAZGXZ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.437"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..163)
                     /locus_tag="ACHKJF_005681"
     CDS             complement(<1..163)
                     /locus_tag="ACHKJF_005681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_146236967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFP0952814.1"
                     /translation="MNKKEIIILLIARILQILISLFTMRLMTTILSNEQMGEYYIFLA
                     IYMLASLGII"
     gene            complement(160..>597)
                     /locus_tag="ACHKJF_005682"
     CDS             complement(160..>597)
                     /locus_tag="ACHKJF_005682"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP0952815.1"
                     /translation="KCCYIGKNVYITKSIIGNYTSIANNVSIGQGEHKVDAVSTSSMF
                     YDNEFEDLTQNDCIIGNDVWLGTGVTVLRGVRIGDGAIIGANAVVTKNIPDYCIAVGV
                     PAKVIKRRLPVNVTEKIKETKWWNDEYEVAKDKIICLTNELKL"
BASE COUNT          188 a          126 c           63 g          220 t
ORIGIN      
        1 taattatacc caatgatgcc aacatataga ttgctaaaaa aatataatac tcccccattt
       61 gctcattgct taaaatcgtt gtcatcaatc tcattgtgaa taaactaatg agaatctgta
      121 gaattctggc tataagcaag ataattattt cttttttgtt cataacttta attcatttgt
      181 taagcaaata attttatcct tcgccacttc atattcatca ttccaccatt tagtctcttt
      241 tattttttca gtaacattta ctggtaatct tcttttgatt actttcgcag gtactcctac
      301 cgcaatacaa taatctggaa tattttttgt gacaacggca ttagcaccaa taatagcacc
      361 atcaccaatt cttactcctc gtaatacagt aactcctgta ccgagccaca catcattacc
      421 tataatacaa tcgttttgtg ttaaatcttc gaactcatta tcgtagaaca tcgaacttgt
      481 actaactgca tcaaccttat gttcaccttg accaattgag acattatttg caatagatgt
      541 gtaattccca atgatacttt tagttatata tacatttttc cctatataac aacactt
//
LOCUS       DAZGXZ010000435          588 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000435 DAZGXZ010000000
VERSION     DAZGXZ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.438"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>588
                     /locus_tag="ACHKJF_005683"
     CDS             <1..>588
                     /locus_tag="ACHKJF_005683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952816.1"
                     /translation="RMEPVGECREDAGTYRCLPEPEAVLTEAEEECLNELMARYDALE
                     NQCEESDLLAAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNVCVQRGVQLRSEDDA
                     AADADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPDKSLTLLAWTLCLNV
                     FGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMA"
BASE COUNT          136 a          144 c          206 g          102 t
ORIGIN      
        1 cgtatggagc ctgtcggtga gtgccgtgag gatgccggaa cataccgctg tctgccggag
       61 ccggaagcgg tgctgacgga ggcagaagaa gaatgcctga acgaactgat ggcacgttac
      121 gacgcactgg aaaaccagtg tgaggaatcc gacctgctgg cagcagaaat gaagctgatg
      181 cgctgcatgg cgaaggtcag ggcgtggacg ccggagatgc gtgccggaag cggtgtggtg
      241 gtgtcctggc gttatggcaa cgtgtgtgtc cagcgtggtg tgcagttgcg cagcgaggat
      301 gacgcggctg ccgacgctga ccgcacggaa caggtgcagg agaaagcgtc agtggaggaa
      361 atcagtctgc cgctgctgac gaaaatgtct tccgaacgca cgctggcagt ccaggcggca
      421 ctgatgcagc aaccggacaa atccctgaca ctgctggcat ggacgctctg cctgaatgtg
      481 tttggcagcg gagcgtacag taaaccagca caaatcagcc tggaatgtaa acattattcg
      541 ctgaccagcg atgcgccgtc agggaaggaa ggtgccgcgt tcatggcg
//
LOCUS       DAZGXZ010000436          586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000436 DAZGXZ010000000
VERSION     DAZGXZ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.439"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..229
                     /locus_tag="ACHKJF_005684"
     CDS             <1..229
                     /locus_tag="ACHKJF_005684"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0952817.1"
                     /translation="LKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMD
                     DEQFDEINIVRGQPVSGGRLEIRITEVGVHE"
     gene            222..>586
                     /locus_tag="ACHKJF_005685"
     CDS             222..>586
                     /locus_tag="ACHKJF_005685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0952818.1"
                     /translation="MNNQYLQFVREQLMIATADLSGSTKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIQGKQSLAKGSSIALVSPVEFATSSWRRALLELEEHQK
                     AWLLWCYGGNICWEHQIAIT"
BASE COUNT          162 a          121 c          168 g          135 t
ORIGIN      
        1 gttgaaatta agcctgtccg gacggctggc aataaaaatt attgcagagc caccggataa
       61 gcgccgccgt gacctggaca atattctgaa agcaccgctg gatgcgctga cgcacgcggg
      121 gttgttaatg gacgatgaac agtttgatga aatcaatatt gtacgtggtc agccagtatc
      181 tggtggacgg ctggaaataa gaattacaga ggtgggtgtg catgaataac cagtatttac
      241 agtttgttcg tgagcaactc atgattgcta ctgcagatct cagtgggtcg acaaaaggcc
      301 agctggaagc ctggcaggaa aatgccctgt tcgatacagg gcgttacaga cgcaaaaaaa
      361 ttcgttaccg cgatgaggta actggaaaaa tgatcacgcg ggataatccc ccgatccagg
      421 gtaaacaatc actggcgaaa ggctcatcaa ttgcgctggt cagtcctgtt gagtttgcaa
      481 catcatcgtg gcggcgtgcc cttctggaac tggaagaaca tcagaaggcg tggttgttgt
      541 ggtgttatgg cggaaacatt tgctgggagc atcagatcgc gataac
//
LOCUS       DAZGXZ010000437          580 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000437 DAZGXZ010000000
VERSION     DAZGXZ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.440"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            24..98
                     /gene="tap"
                     /locus_tag="ACHKJF_005686"
     CDS             24..98
                     /gene="tap"
                     /locus_tag="ACHKJF_005686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP0952819.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            91..>580
                     /gene="repA"
                     /locus_tag="ACHKJF_005687"
     CDS             91..>580
                     /gene="repA"
                     /locus_tag="ACHKJF_005687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator RepA"
                     /protein_id="HFP0952820.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SED"
BASE COUNT          116 a          162 c          161 g          141 t
ORIGIN      
        1 agctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt ctgtgctcgc
       61 tccttctgcg cattgtaagt gcaggatggt gtgactgatc ttcaacaaac gtattaccgc
      121 caggtaaaga acccgaatcc ggtgtttaca ccccgtaaag gtgccggaac gctgaagttc
      181 tgcgaaaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt cgccattcat
      241 gtggcgcatg cccgttcgcg tggtctgcgt cgacgcatgc caccggtgct gcgtcgacgg
      301 gctattgatg cgctgctgca ggggctgtgt ttccattatg acccgctggc caaccgcgtc
      361 cagtgttcca ttaccacact ggccattgag tgcggactgg cgacagagtc cggtgcagga
      421 aaactctcca tcacccgggc cacccgggcc ctgacgttcc tgtcagagct gggactgatt
      481 acctaccaga cggaatatga cccgcttatc gggtgctaca ttccgaccga tatcacgttc
      541 acaccggcgc tgtttgccgc ccttgatgtg tctgaggatg
//
LOCUS       DAZGXZ010000438          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000438 DAZGXZ010000000
VERSION     DAZGXZ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.441"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..571
                     /locus_tag="ACHKJF_005688"
                     /pseudo
     CDS             32..571
                     /locus_tag="ACHKJF_005688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          161 a          119 c          155 g          136 t
ORIGIN      
        1 atatatgtct aacactagtg ctaattttga gatgactgga attttgctgg ggcaggagct
       61 tcgtaaacgt aaaactcctc aggaaaaaat cgccattatt cagcagacaa tggaacccgg
      121 tatgacggtg tcccatgtgg cgcgtttgca tggtattcag cccagcctgt tgtttaagtg
      181 gaaaaaacaa tatctggaag gaagtctcac tgctgttgct gccggagagg atgtcgttcc
      241 tgcctcagaa ctggctgccg ctataaaaca aataaaagag ttacagcgct tgctgggtaa
      301 gaaaaccatg gaagttgaga tcctcaaaga agcggtggag tatggtcagt cgcgaaaatg
      361 gatagcgcat gcgcccttgt tgccaacgga caaggaataa ccctggtaag ccgtattctg
      421 ggcgtgtcgc gtgcgcaact ggcacaacgg atgaaaacat ctggtaataa gccgaataaa
      481 cgtcgtcgca gatgtgatga ggctgcggat gcggaagtgt tagcacgtat ccttgatatc
      541 atcagcgata tgccgacata tggctatcgt c
//
LOCUS       DAZGXZ010000439          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000439 DAZGXZ010000000
VERSION     DAZGXZ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.442"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(82..571)
                     /locus_tag="ACHKJF_005689"
                     /pseudo
     CDS             complement(82..571)
                     /locus_tag="ACHKJF_005689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          171 a          143 c          109 g          148 t
ORIGIN      
        1 gactttaaag ttgcaccaat tattctattt aactaaaaaa acataaagtc aagcacatat
       61 aacttgtagt ggatcacaaa atcaggacac cgggataacc tgtttccact tttcttcatg
      121 taagcaaggc ggtaaaccac cgttgttcgt gtgaggtcga taaacgttgt aataaccggt
      181 ttatatctcg taccgcatgg ctaaaatcac cataaccacc tttcggaagc cattcacttt
      241 taaggctgcg aaagactctt tccatcagcg aattatccag gcaattccct ctgcgactca
      301 tactttgcat taccccataa cgccagagta actttctgta tttattgctt ttatactgaa
      361 cacattgctc tgaatcaaac agcaggcggt catcacgcgg tcgtgtttcc agtgcattac
      421 gcaaagccct gcacaccaac tcagcatcag cggttaatgg gagggctgaa ccgataatcc
      481 gacgtgaata taaatcaaca acgagcgcga gataacacca tttgtcctgc aggcgaataa
      541 aactgatgtc gccgcaccag accgcgtctg g
//
LOCUS       DAZGXZ010000440          566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000440 DAZGXZ010000000
VERSION     DAZGXZ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.443"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>566
                     /locus_tag="ACHKJF_005690"
     CDS             <1..>566
                     /locus_tag="ACHKJF_005690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0952821.1"
                     /translation="EQPLVLTNVAAGNVSPVSTDAVNGSQLASAARIAGGDNVVWKDG
                     KAALAPDTFSELTTAGGGKTTVNGGVHKTPDNVADAVNMLNREGTKYFKAASSGQAAK
                     AEGRDSIAVGQGAVSRGQNSIAMGNGAEVTRQAAAGSVAVGSHARAGRANTGTYALNG
                     QAVAGRTGSDTAVVSFGQPGQERQLQSVA"
BASE COUNT          126 a          131 c          189 g          120 t
ORIGIN      
        1 gagcagcctc ttgttctgac aaatgtggcg gccggaaatg tgtcaccggt atccacggat
       61 gcggttaacg ggagccagct ggcatctgca gccagaattg ctggcggtga taatgttgtc
      121 tggaaagacg gaaaagcagc gctggctcct gacaccttca gtgagctgac aactgcaggt
      181 ggcggtaaga caacggtgaa tggtggtgta cataaaacac cggataatgt ggctgatgca
      241 gtgaatatgc tgaaccgtga aggcacgaag tattttaaag ctgcctcttc cggtcaggca
      301 gcaaaagcgg aagggcgtga cagtattgct gtcggacagg gggccgtttc ccggggacaa
      361 aacagcattg ccatgggtaa tggtgctgaa gtcacgcgtc aggctgctgc cggctctgtt
      421 gcagttggca gccatgcccg tgctggccgc gcgaataccg ggacttatgc cctgaacggt
      481 caggctgttg cgggacgtac cggaagtgat actgcggtgg tttcttttgg tcagccggga
      541 caggagcgtc agctgcagtc agtggc
//
LOCUS       DAZGXZ010000441          557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000441 DAZGXZ010000000
VERSION     DAZGXZ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.444"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          184 a          117 c          101 g          155 t
ORIGIN      
        1 ccagctaatg ccccatgcag ccagcataac cagcataaac tgcagatatt gattacgtgt
       61 ctgaatcatt gcatctcctg taaattttta acttatccta ttcttgtcat tactacgtat
      121 atacatatct ataacaattc agatatcgtc accaggatat gccgcaccag cggcatggaa
      181 ggcggcactc agttgttgca tatgataccg gcgtaagccg gtactgattg acaggtttca
      241 ccttacccac ccccggccct gccagaccag tgggggctta aaggggtagt gtgacatcct
      301 gacagcaact gaaagcaaaa tgtaaacaca atattaattt gtgtaaaaaa acaataaccg
      361 cgcaaacata accaactgat attattatca aatatttaac aggcgtacaa atttagttca
      421 aattatcatt gcaaaatata gttaattacg gcaagtaact agtgttggcc aacatactat
      481 tcagatgtca taagcattaa tttcccttaa aaaaggagtc cttatggaat taaaagcgag
      541 tgaatttggt gtagttt
//
LOCUS       DAZGXZ010000442          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000442 DAZGXZ010000000
VERSION     DAZGXZ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.445"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            252..>549
                     /locus_tag="ACHKJF_005691"
     CDS             252..>549
                     /locus_tag="ACHKJF_005691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952822.1"
                     /translation="MWWLVTAPVRELARNALLMAVWRVSLHKTLLYYVYQRVTRLEQP
                     VQKCPRSEHGKFSPYNGFLTPLLFSFSHINDGVYSDGRVFRLCQSSGLSCSFVHA"
BASE COUNT          137 a          133 c          125 g          154 t
ORIGIN      
        1 acctgcgcca catcgacaga atgttttggc cccgacagga aaaagaacta ataaatgatg
       61 ctcctgccca gatatatact gggcgggagc atactttggt tgcgacagaa atatgtcaac
      121 accacgaact ttgacattac gtcttatatc acactggcac tgacaagcca gtattgctaa
      181 tgcaggagga tgctttttca actcgcagga gtttcaggag ctgcctgatg gcgctgaaat
      241 gatgcttact catgtggtgg ctggtcactg cccctgtccg cgagctggca cgcaatgcac
      301 tactgatggc agtctggcgc gtaagcctgc ataaaacatt attgtactat gtgtatcaac
      361 gggttacccg gttagaacag ccagtacaaa aatgcccccg cagtgagcat ggcaagtttt
      421 ccccatacaa tgggtttctc acccctcttt tgttttcttt ttctcatata aatgatggtg
      481 tttactcaga cggaagagta tttcgtctgt gccagtcttc cggattatcc tgctcatttg
      541 ttcatgcat
//
LOCUS       DAZGXZ010000443          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000443 DAZGXZ010000000
VERSION     DAZGXZ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.446"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..445)
                     /locus_tag="ACHKJF_005692"
     CDS             complement(<1..445)
                     /locus_tag="ACHKJF_005692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0952823.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLREEGFQPFFACQSRVRDLGRREYSKHMLRLRREEHINGQEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQV"
BASE COUNT          177 a          128 c           98 g          146 t
ORIGIN      
        1 taacctgccc gacaatatca cctttatgtg gaacgcggat ttcgccaaaa ttatttccgc
       61 acaccagacc atttgtgcag acaaaacgaa aaattcctgg gatcatctga taactggatg
      121 aaccatcatg tgaattaagc aggataattt caggaacttc ctgcccgtta atatgctctt
      181 ccctgcgaag acgtaacata tgtttactgt attcgcggcg tcccaaatcc cgaacccgac
      241 tctgacatgc aaagaacggc tggaaacctt cttcacgtaa cttattgatg atattgattg
      301 ttggaatata tgtataacgc tcactccggg attcatgttt atcaccggaa aataccgaag
      361 gcacaaactg cattaattca tcatccgtta aaggacgctc acggcgaatg gaattatacc
      421 gaccaaaacg ggaagctaat cgcataacag ctactccttt atgaaagaat aaatttaata
      481 aaaaattttt ccgcttaata tcatccctcc cggtcttcat taacaagatt gcaacaatca
      541 ggagggata
//
LOCUS       DAZGXZ010000444          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000444 DAZGXZ010000000
VERSION     DAZGXZ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.447"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>549
                     /locus_tag="ACHKJF_005693"
     rRNA            <1..>549
                     /locus_tag="ACHKJF_005693"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          157 a          129 c          154 g          109 t
ORIGIN      
        1 ttccaggaaa agcctctaag catcaggtaa catcaaatcg taccccaaac cgacacaggt
       61 ggtcaggtag agaataccaa ggcgcttgag agaactcggg tgaaggaact aggcaaaatg
      121 gtgccgtaac ttcgggagaa ggcacgctga tatgtaggtg aagtccctcg cggatggagc
      181 tgaaatcagt cgaagatacc agctggctgc aactgtttat taaaaacaca gcactgtgca
      241 aacacgaaag tggacgtata cggtgtgacg cctgcccggt gccggaaggt taattgatgg
      301 ggtcagcgca agcgaagctc ttgatcgaag ccccggtaaa cggcggccgt aactataacg
      361 gtcctaaggt agcgaaattc cttgtcgggt aagttccgac ctgcacgaat ggcgtaatga
      421 tggccaggct gtctccaccc gagactcagt gaaattgaac tcgctgtgaa gatgcagtgt
      481 acccgcggca agacggaaag accccgtgaa cctttactat agcttgacac tgaacattga
      541 gccttgatg
//
LOCUS       DAZGXZ010000445          537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000445 DAZGXZ010000000
VERSION     DAZGXZ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.448"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            124..>537
                     /locus_tag="ACHKJF_005694"
     CDS             124..>537
                     /locus_tag="ACHKJF_005694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HFP0952824.1"
                     /translation="MDFSTLITFSIITLSQTVSIGPGVALIISESLTYGAKKAITTSI
                     YIRFGEIVVMLVTLFSIALAINLEKHLDTIKTCGGGYLIFLGITRLIKRKGSKGNKAN
                     IPFLIPMLNPKAFLFFAAFMPSFIKNNSNFNTDFLL"
BASE COUNT          181 a           98 c           85 g          173 t
ORIGIN      
        1 tagctgccag caattcgcaa aaaaacattt ctgactttat aatgttgctt cctgaggcag
       61 ccatacctat ggagtatgtt tattttaagg ttccaaccag aataacagga gttatataat
      121 cccatggact tctcaacatt aataacattc tctattataa ccttatcaca aacagtatcg
      181 attggtccgg gggtggcatt aatcatatct gaaagtctga catatggtgc aaaaaaagca
      241 ataacgacaa gtatatatat aagatttggc gaaattgttg tcatgctagt cacactattt
      301 tctatagcac tggcaatcaa tttagaaaaa catcttgaca caataaaaac ttgtggtggt
      361 ggatatctga tttttcttgg tattactcgt ctcatcaaga gaaaaggatc taaaggaaac
      421 aaagcaaata tccctttttt aataccgatg ctcaatccaa aggccttttt gttctttgct
      481 gcatttatgc catcattcat aaaaaacaac agtaacttta atactgactt tttgtta
//
LOCUS       DAZGXZ010000446          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000446 DAZGXZ010000000
VERSION     DAZGXZ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.449"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>511
                     /locus_tag="ACHKJF_005695"
     CDS             <1..>511
                     /locus_tag="ACHKJF_005695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952825.1"
                     /translation="NGADRLVSGTEIQTKYCSTAARSVGAAFDGQNGQYRYMGNNGPM
                     QLEVPRDQYAGAVETMRNKIREGKVPGVTDPAEASRLIRRGHLTYTQARNITRFGTIE
                     SVTYDIAEGSVVSLAAGGISFALTASVFWLSTGDRDAALQTAAVQAGKTFTRTLAVYV
                     TTQQLHRLSV"
BASE COUNT          124 a          143 c          142 g          102 t
ORIGIN      
        1 gaaatggagc agacagactg gtcagcggaa cagaaatcca gacgaaatat tgttcaactg
       61 cagcccgtag cgtcggtgcg gcattcgacg gacaaaacgg acagtatcgt tacatgggaa
      121 ataatggccc catgcaactg gaagtccccc gtgatcagta tgccggcgct gttgaaacca
      181 tgaggaataa gatccgcgaa ggtaaagtac ccggtgtaac cgatcccgca gaagcgtccc
      241 ggctgattcg tcggggacat ctgacttata ctcaggcccg taatatcacc cggttcggga
      301 ccatcgaatc ggtcacttat gatattgccg agggttcggt tgtcagtctg gcggccggag
      361 ggatcagttt tgccctgacg gcatcggtct tctggctcag caccggcgat cgcgatgctg
      421 ccctgcagac agctgctgtc caggcaggaa aaaccttcac ccgcacactg gctgtctacg
      481 tcacaaccca gcaactccac cggctcagtg t
//
LOCUS       DAZGXZ010000447          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000447 DAZGXZ010000000
VERSION     DAZGXZ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.450"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>500
                     /locus_tag="ACHKJF_005696"
     CDS             <1..>500
                     /locus_tag="ACHKJF_005696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFP0952826.1"
                     /translation="SDQRWCSDGFEFGCDDGAKLRVTFALDCCDREAIDWAASTGGYD
                     KATVQDVMLGAIEKRFGDKGAERPIQWLTDNGSAYTAHETRQFARELNLEPCTTAVSS
                     PQSNGMAERFVKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNETHPHSALGYLSPREY
                     RRQRVT"
BASE COUNT          142 a          111 c          146 g          101 t
ORIGIN      
        1 aaagcgacca gcgctggtgt tccgatggct ttgagttcgg ttgtgatgat ggcgcaaaac
       61 tgcgtgtcac gttcgcactg gactgttgcg atcgtgaagc catagactgg gccgcaagca
      121 cgggaggtta tgacaaagca acggtgcagg atgtcatgtt aggagcaata gaaaaacgct
      181 ttggcgataa aggagcggaa cggccaatcc agtggctgac tgataacggt tcggcttata
      241 cagctcatga aacacggcag ttcgccagag agttgaatct ggagccctgc acgacggcag
      301 tcagtagccc tcagagcaat ggaatggcag aaaggttcgt gaaaacgatg aaagaagact
      361 acatcgcgtt catgccgaaa ccgaatgtaa gaacggcatt gcataacctt gcagtggcga
      421 tcgagcatta caatgaaacc catccgcata gtgcgttagg ttatctctca ccaagggaat
      481 atcgacgtca gcgggtaacg
//
LOCUS       DAZGXZ010000448          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000448 DAZGXZ010000000
VERSION     DAZGXZ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.451"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>485
                     /locus_tag="ACHKJF_005697"
     CDS             <1..>485
                     /locus_tag="ACHKJF_005697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0952827.1"
                     /translation="SAGASENSLRWGVGKPLYQDLVSRTKAALAKNPKNRLLAVVWMQ
                     GEGDAAVGTHAQHPGLFSAMVNQFRTELAGQASQSTGGSASAVPWICGDTTYFWKQRH
                     AEGYASVYGGYKGKESQNIFFVPLMTDENGANVQTNDPAEDPDLEAVGYYGSKWRNDQ
                     KT"
BASE COUNT          121 a          101 c          167 g           96 t
ORIGIN      
        1 tcagccggtg catcggaaaa ttcactgcgc tggggtgtgg gtaagccgct gtatcaggat
       61 ttggtgagcc ggactaaagc cgcactggcg aagaacccga aaaaccgtct gcttgcggtg
      121 gtgtggatgc agggagaagg tgatgcggca gtggggacgc atgcgcagca tccggggctg
      181 tttagtgcga tggtgaatca gttcagaacg gagcttgccg gtcaggcatc acagagcaca
      241 ggaggcagcg catcagcggt accgtggatt tgcggggaca caacgtattt ctggaagcag
      301 cgtcatgcgg aagggtatgc atcagtatac ggagggtata agggtaagga gtcgcagaac
      361 attttctttg taccgttaat gacggatgag aatggtgcga atgtgcagac caatgacccg
      421 gcagaagacc cggacctgga agccgtcggt tattacggtt caaagtggcg taatgaccag
      481 aagac
//
LOCUS       DAZGXZ010000449          472 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000449 DAZGXZ010000000
VERSION     DAZGXZ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.452"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>472
                     /locus_tag="ACHKJF_005698"
     CDS             <1..>472
                     /locus_tag="ACHKJF_005698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0952828.1"
                     /translation="HDIIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGKTLR
                     HSYDKSRRRGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLNMMDIKGKIITTD
                     AMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSE"
BASE COUNT          160 a           88 c          108 g          116 t
ORIGIN      
        1 cacgatatca ttgccagagt tgtatcctgt atcagtcctg caaaatttca cgagtgcttt
       61 attaactgga tgcgtgactg ccattcttca gatgataaag acgtcattgc aattgatgga
      121 aaaacgctcc ggcactctta tgacaagagt cgtcgcaggg gagcgattca tgtcattagt
      181 gcgttctcaa caatgcatag tctggtcctc ggacagatca agacggatga gaaatccaat
      241 gagattacag ccattcctga acttcttaac atgatggata ttaaaggaaa aatcatcaca
      301 actgatgcga tgggctgcca gaaagatatt gcagaaaaga tacaaaaaca gggaggtgat
      361 tatttattcg cggtaaaagg aaaccagggg cggctaaata aagcctttga ggaaaaattt
      421 ccgctgaaag aattaaataa tccagagcat gacagttacg caatgagtga aa
//
LOCUS       DAZGXZ010000450          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000450 DAZGXZ010000000
VERSION     DAZGXZ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.453"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..325
                     /locus_tag="ACHKJF_005699"
     CDS             <1..325
                     /locus_tag="ACHKJF_005699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="RepA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0952829.1"
                     /translation="QRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSHGMKRARA
                     RRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIMSRNNNYTRL
                     ATASP"
BASE COUNT          117 a          137 c          129 g           84 t
ORIGIN      
        1 acagcgcaaa aagcaggggc tggataccct gggtatggat gagctgatag cgaaagcctg
       61 gcgttttgtc cgtgagcgtt tccgcagtta ccagacagag cttaagtccc acggcatgaa
      121 acgtgcccgt gcgcgccgcg atgcaggcag aacccgccag gacattgtca ccctggtgaa
      181 acggcagctg acgcgtgaaa tcgcggaagg gcgtttccgg ggaagcctgg aggccgtgaa
      241 gcgcgaaatc gaccgtcgtg tgaaggaacg catgataatg tcgcggaaca acaactacac
      301 acggctggcc actgcctccc cctgagtttt acctcatctg aataatccgg cctgcgccgg
      361 aggcatctgc acgcctgaag cctgtcagcg aacaaaaaaa cagcaccaca tacaaaaaac
      421 aatctcatca tcccgcttct ggtgcatccg gcccccctgt tttcgat
//
LOCUS       DAZGXZ010000451          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000451 DAZGXZ010000000
VERSION     DAZGXZ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.454"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>467)
                     /locus_tag="ACHKJF_005700"
     CDS             complement(<1..>467)
                     /locus_tag="ACHKJF_005700"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0952830.1"
                     /translation="EGDFTQEADRIGVKTYVIETLVREISPLKDFYSLIKLYKFIKQE
                     KFDIIHTHSSKSGFLGRLAAKLAGTKQIIHTVHGFAFPSTSNRVVKLIYFLMEYFASL
                     CSSVIIVMNENDEKIARKYFSSAPWTKVTLLNNAVDIKKFQKRYIGIESKSEI"
BASE COUNT          145 a           78 c           70 g          174 t
ORIGIN      
        1 tttatttcac tttttgattc gattccaata tatcgctttt gaaacttctt tatatcaaca
       61 gcattgttta agagagtcac tttggtccaa ggagcagaag aaaaatattt tctggctatt
      121 ttctcatcat tctcattcat tacgataatc actgatgaac ataatgaagc aaagtattcc
      181 attaaaaaat atataagctt tacaactcta tttgatgttg aaggaaacgc aaatccatgc
      241 accgtatgga ttatttgttt tgtgcccgcg agttttgctg ctaatcgccc gagaaaacca
      301 ctttttgagg aatgcgtatg aataatatcg aatttttctt gcttgataaa tttatataat
      361 tttattaatg aataaaagtc ttttagaggt gaaatttctc tgactaaggt ttctattaca
      421 taagttttta ctccaatacg atccgcttct tgagtaaaat cgccttc
//
LOCUS       DAZGXZ010000452          466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000452 DAZGXZ010000000
VERSION     DAZGXZ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.455"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>466
                     /locus_tag="ACHKJF_005701"
     CDS             <1..>466
                     /locus_tag="ACHKJF_005701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952831.1"
                     /translation="IDFSTASPTVRLALQKGTGAGNISALNKVMKGTLVTSLALVAVT
                     TGPDMIKMLRGRISGAQFIRNLAVASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVG
                     GVLGGMIASAVSGKIAGALVEEDRVKILAMIQEQMTWLAGSFLLTGHEIENLN"
BASE COUNT          101 a          104 c          151 g          110 t
ORIGIN      
        1 attgattttt cgactgccag cccgactgtc cgactggctc ttcagaaggg gaccggtgca
       61 ggaaatatca gtgccctgaa caaagtaatg aagggtacgc tggtgacatc tctggcactg
      121 gtagctgtca caaccggccc tgacatgatc aaaatgttgc ggggacggat ctccggtgcg
      181 cagttcatca ggaatcttgc cgtggcatct tccggtgtgg caggtggagc tgtcgggtca
      241 gtggcgggcg ggatactgtt cagtccactg ggaccatttg gtgcactgac agggcgtgtg
      301 gttggcggtg ttctgggggg aatgattgcc tccgctgtat caggaaaaat tgccggagcg
      361 ctggttgaag aagatcgcgt caaaattctg gcaatgattc aggagcagat gacatggctt
      421 gccggcagtt tcctgctgac cggacatgag attgaaaatc tgaacg
//
LOCUS       DAZGXZ010000453          456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000453 DAZGXZ010000000
VERSION     DAZGXZ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.456"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(223..>456)
                     /locus_tag="ACHKJF_005702"
     CDS             complement(223..>456)
                     /locus_tag="ACHKJF_005702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0952832.1"
                     /translation="NHEEMKRAAAQSAALAGLFQPYSVGKFNATAAVGGYSDRQAVAV
                     GMGYRFNEQAAAKAGTAFSDGDVSWNVGVNWEF"
BASE COUNT          109 a          159 c           98 g           90 t
ORIGIN      
        1 acagctgcat cgtcgcattc cagacgaacc tgcaccctgt cagcccgacc gtttacagta
       61 aatacctgca ctgaacttat gcgatagttc agcatataca ctctccctct cagctcctca
      121 ggaatacccc ggaaccgtca gtcatgccac cggggagaaa agcaggcagc gaatcatgac
      181 cgcatacaga acgctgcccg ccccggtgac tcagacccgg gttcagaact cccagttgac
      241 acccacattc caggaaacat caccgtcact gaacgcagta cctgcctttg ccgcagcctg
      301 ctcgttaaag cgatatccca tgcccaccgc aacagcctgc ctgtcactgt acccgcccac
      361 agccgctgtg gcgttaaatt tacccacact gtatggctgg aaaagaccgg ccagcgcagc
      421 tgactgggct gctgcccgct tcatttcctc atgatt
//
LOCUS       DAZGXZ010000454          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000454 DAZGXZ010000000
VERSION     DAZGXZ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.457"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>455
                     /gene="mobF"
                     /locus_tag="ACHKJF_005703"
     CDS             <1..>455
                     /gene="mobF"
                     /locus_tag="ACHKJF_005703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MobF family relaxase"
                     /protein_id="HFP0952833.1"
                     /translation="EALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAV
                     VANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVG
                     KHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHV"
BASE COUNT          115 a          105 c          154 g           81 t
ORIGIN      
        1 ggaggcgctg gcctccacac gggtgatgac ggacggacag tcagaaacgg tactgaccgg
       61 taatctggtg atggcactgt ttaaccacga caccagtcgc gatcaggaac cacagttaca
      121 cacgcatgcg gtggtggcta atgtcacgca gcataacggt gagtggaaga cactgagcag
      181 tgacaaagtg gggaaaacgg ggttcattga gaatgtgtac gctaatcaga ttgcctttgg
      241 caggctctac cgggaaaaac tgaaagagca ggttgaggcg ctgggctatg aaaccgaagt
      301 ggtcggtaag cacggtatgt gggagatgcc gggcgtaccg gtggaggcct tttccggacg
      361 ctcacaggct atccgggagg ccgtcgggga ggatgcctcg ctgaaatccc gggatgtggc
      421 ggcactggat acgcgaaaat ccaaacagca cgtcg
//
LOCUS       DAZGXZ010000455          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000455 DAZGXZ010000000
VERSION     DAZGXZ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.458"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..432
                     /locus_tag="ACHKJF_005704"
     CDS             <1..432
                     /locus_tag="ACHKJF_005704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0952834.1"
                     /translation="GRDYQIDPDLLRAISWKESRNRVNAIGINPVTGYGSGLMQVDSQ
                     HFNELARYGIKPEHLTADPCLNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQN
                     QRRLAYASEVYRIYTGIKSSKGIRIPVTKKSLPEINSVQNN"
BASE COUNT          137 a          103 c          109 g          106 t
ORIGIN      
        1 ggccgggatt accaaataga tcctgattta ctgagagcaa tatcatggaa agaatcccgt
       61 aaccgggtta atgccatcgg tattaacccg gttacgggat atggcagtgg acttatgcag
      121 gtagactccc agcattttaa cgaactggcc cgctacggaa ttaaaccgga acatctgaca
      181 gcagatccct gtctgaacat ttataccggt gcttattatc tggctatagc ctttaaaaaa
      241 tggggagtct cctgggaggc tgttggtgca tacaacgcag gattcaggaa aaccgaacgc
      301 cagaaccagc ggcgtcttgc ctacgcatca gaggtttacc ggatttatac cgggataaaa
      361 agcagtaaag gtatccggat tccggtcacg aagaaatcac ttccggaaat taacagcgtg
      421 cagaacaatt aatcttacag acattgtcag atacc
//
LOCUS       DAZGXZ010000456          448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000456 DAZGXZ010000000
VERSION     DAZGXZ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.459"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            328..447
                     /locus_tag="ACHKJF_005705"
                     /pseudo
     CDS             328..447
                     /locus_tag="ACHKJF_005705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          121 a          116 c           79 g          132 t
ORIGIN      
        1 ataagcaata aaaaacagct tcaggtaatg aggaatatct caatttttaa acataaaatg
       61 cgaattattt agtacaaaaa gtgaactttt gatgatcttt ccctaatctc acataggatc
      121 agatctcgtc tgatcgttcc ccctgcgctc actaccatgg ctctttacca cagcagccgg
      181 ggttgtttgg gaccgacttc cgacagcaga tcccggtggg cctgccacca tctcttcaca
      241 agcttatgct tattacatgc aaaacgtccc gttttgcatc tttgtgccta cggcacaccg
      301 atgatctttc cctgtgatct ttccctgaat ctgcccctga gaacctgaat caaacgtctg
      361 actcgcagaa ccatctgttt ctcgcgctct gtagaacttc atgaaaaagt catcggtgct
      421 tttatcgaaa aatacatgtt ctactaat
//
LOCUS       DAZGXZ010000457          443 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000457 DAZGXZ010000000
VERSION     DAZGXZ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.460"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>443
                     /locus_tag="ACHKJF_005706"
     CDS             <1..>443
                     /locus_tag="ACHKJF_005706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952835.1"
                     /translation="IINPPDDIRTNKIIKNHLRIMEKYTRESCEKRFIYFLGSRKKVR
                     FSTKKQPRYNPFTKNIVFTLLIGKEERKLRIKYSFCTFTQNGVVKVTPNVRVTEKFLY
                     IEYENGNLCVMSVHDFVNGHYIDLGIHTEVHYVGYTRHPHRRPVD"
BASE COUNT          163 a           66 c           86 g          128 t
ORIGIN      
        1 ataattaatc ctcctgatga tataagaaca aataagataa taaagaacca tctcagaatt
       61 atggaaaaat atacgaggga aagctgtgaa aagagattta tttatttctt aggttcaaga
      121 aaaaaagtga ggttttcaac caagaaacag cctaggtata acccattcac aaaaaatata
      181 gtattcactc tgttgattgg caaagaagag cgaaagcttc ggataaaata ttccttttgc
      241 acgtttacgc agaatggggt agtgaaggta actccaaatg ttagagtaac tgagaagttt
      301 ctctacattg aatatgaaaa tggcaattta tgtgtaatgt cagttcatga cttcgtaaac
      361 ggacattata ttgatttagg aattcataca gaagtgcatt acgtaggata tacacgtcat
      421 ccgcatcggc gccctgttga ttt
//
LOCUS       DAZGXZ010000458          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000458 DAZGXZ010000000
VERSION     DAZGXZ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.461"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..173)
                     /locus_tag="ACHKJF_005707"
     CDS             complement(<1..173)
                     /locus_tag="ACHKJF_005707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0952836.1"
                     /translation="MANRKQQRARAERLHTRTEINRRLWRATRVAQIMHINMLHERTH
                     ALSNRYCAAVFSYL"
BASE COUNT           95 a          111 c          141 g           95 t
ORIGIN      
        1 agatagctga agacagcagc gcagtaacgg tttgatagtg cgtgcgtgcg ctcatgcagc
       61 atgttgatgt gcatgatttg cgcgacgcgt gttgcgcgcc agagtctgcg gttgatttca
      121 gtacgggtgt gaagacgctc agcgcgggcg cgctgttgct tgcggtttgc catggtgtgg
      181 cctctttgct agtaagttct gaaaactcac caacctgagg cggtaatctc agggtggcga
      241 gacgtacagg gttaccacaa ccggctagca aagaatccgg cccgaccgaa gtcggccccg
      301 tacgccccgc cataattcgt gtgcgaaaaa gacgtggcga tacagtacgc acaaaaaaac
      361 cgctggcgcg gctgtgcgct ttgctagtcg gcggggtggt aatcccggca cccgttttat
      421 gaggtgcagc ggaaatgtaa cc
//
LOCUS       DAZGXZ010000459          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000459 DAZGXZ010000000
VERSION     DAZGXZ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.462"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            260..439
                     /gene="hha"
                     /locus_tag="ACHKJF_005708"
                     /pseudo
     CDS             260..439
                     /gene="hha"
                     /locus_tag="ACHKJF_005708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
BASE COUNT          118 a           94 c          119 g          110 t
ORIGIN      
        1 gcgctatccc tgatagctgg cgtataaccc gcgtatataa aacgtatctg caatgtacgt
       61 gaacagagtg cattaagcat ccgtctgagc ggtaaacata tccgacctgg tgagtgacag
      121 tctgagagac cggttaagtg gtggctcggg agtaatgagt ttggggtggg taccagaatg
      181 ccagtctggc ggtatgctga cagtatcacg ttaattgcag cataaagtga tgagaggcta
      241 atggaagtta aaaccaaagc agttccgtcg ttgttcatcc cgggagacgc tggaaaaagt
      301 gatttcccac acgcgttata aacttacctc tgcggaactg gaagccttca actctgtggt
      361 tgatcactgg ctggcagaac tgacaatgaa caagctttac gatcgtgttc ccgcttcagt
      421 ctggaaatat gtcatctgag c
//
LOCUS       DAZGXZ010000460          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000460 DAZGXZ010000000
VERSION     DAZGXZ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.463"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>434
                     /locus_tag="ACHKJF_005709"
     CDS             <1..>434
                     /locus_tag="ACHKJF_005709"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952837.1"
                     /translation="PVSVMAKAQKGSVINSITFTDSLTDETDDKHDFTNTGDLKGSTP
                     SIMTFPYGESDDRYTTNYVGNVQSSKTKFINLATGLTNDTTPTILISLDKELNNNQHI
                     EITRYKVDVDNDNILYEVVDEIIPSEHVDIKGKNIIVKDQLE"
BASE COUNT          159 a           64 c           81 g          130 t
ORIGIN      
        1 cccagtcagt gtgatggcaa aagcacagaa aggtagcgtc attaattcca ttaccttcac
       61 tgatagttta actgatgaaa ccgatgataa acatgatttt actaatacag gtgatctgaa
      121 gggcagtacg ccttctatca tgacatttcc gtatggtgaa tcagatgata ggtatacgac
      181 taactatgtt ggtaatgttc agtcctcaaa aacaaaattt ataaatcttg caacagggtt
      241 aacgaatgat actacgccaa ctatattaat atctcttgat aaggagctga ataataatca
      301 acatattgaa attacacgtt ataaagttga tgttgataac gataatatat tgtatgaagt
      361 agttgatgag ataatcccta gtgaacatgt ggatattaaa ggaaaaaata ttattgttaa
      421 agatcaactg gagc
//
LOCUS       DAZGXZ010000461          431 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000461 DAZGXZ010000000
VERSION     DAZGXZ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.464"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>431
                     /locus_tag="ACHKJF_005710"
     CDS             <1..>431
                     /locus_tag="ACHKJF_005710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0952838.1"
                     /translation="VVHTLPGDRYGVAAGGRRLAALNMLAERGIIPADWPVCVKVIPQ
                     ELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIGDLLGYSPRHVQRMLKLAD
                     LAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQSG"
BASE COUNT           92 a          125 c          131 g           83 t
ORIGIN      
        1 gtcgtgcata ccctgcctgg tgaccgttac ggtgtcgccg caggtggtcg ccgactggct
       61 gcactcaaca tgctggcaga gcgtggcatc attccggctg actggcctgt ctgtgtgaag
      121 gtcattccgc aggaactggc gacggcggca tcaatgactg aaaacggtca tcgtcgggat
      181 atgcaccctg ctgaacagat tgccggattc cgcgcaatgg cgcaggaagg caaaacacct
      241 gcacaaatcg gtgatttgct gggctattca ccccgccacg ttcagcgaat gctgaaactg
      301 gcagaccttg caccagtcat actggatgcg ctggcagaag accgcatcac caccgaacac
      361 tgtcaggcgc tggcactgga gaacgacacc gcgcgtcagg tgcaggtgtt tgaagccgcc
      421 tgtcagtcgg g
//
LOCUS       DAZGXZ010000462          430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000462 DAZGXZ010000000
VERSION     DAZGXZ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.465"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..102
                     /locus_tag="ACHKJF_005711"
     CDS             <1..102
                     /locus_tag="ACHKJF_005711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0952839.1"
                     /translation="VRDCWRRQIDKRAAEKISASQSAGSKPEEPITP"
     gene            128..373
                     /locus_tag="ACHKJF_005712"
     CDS             128..373
                     /locus_tag="ACHKJF_005712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0952840.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGEPTPDSDMMM"
BASE COUNT          113 a          108 c          126 g           83 t
ORIGIN      
        1 gtgcgcgatt gctggcgcag gcagattgat aaaagggccg cagagaaaat cagcgccagt
       61 cagtcagccg gaagcaaacc cgaagagccc attactcctt aataacccct ttcaacgaga
      121 aaatcccatg tcagaaatta catccctggt cactgcagag gcagtgaagg aagtcctgcg
      181 ctctgaagaa gtcctgagcg cactgaaaca gaaacttcgc cataacctgg aagcgcgtct
      241 tgatgcagaa gtggatgcca ttctggatga actgctgggc gcaccggcag ctccggagcc
      301 ggaaggcatc gcgggtgagg ggagtgcttc agatagcggt gaacccacac ctgacagcga
      361 catgatgatg taagcatgcg caagggacca tcggtgtgtg ccggtggtct ttatattgtt
      421 gtgagcttcc
//
LOCUS       DAZGXZ010000463          426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000463 DAZGXZ010000000
VERSION     DAZGXZ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.466"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          110 a           94 c          117 g          105 t
ORIGIN      
        1 atttttaatc cccagcatgt cagagggtgc ggatgacggt tcccgtctgg cgttttcatg
       61 ggcgccggtt gtcaaaaaat accagcgtga tcggtcgaag gataagcgca gattaatcag
      121 tgatgattga gcgcgaatct gacaacgagt atagcgacac agcaacaaaa aagacgccag
      181 aaacgcgctc agaggctttg taacgcgatt ttttacatga ggcaatggtt tcgctttgag
      241 gcgttcagcc gtgttcaggg cgcgattctg gtacatacct gactgtaatc atccctacga
      301 actactcttt gcaccagaaa cgcgcagggt gttttagcgt gtctccggcg tgatgtcgag
      361 ttgtgcggta atgagtgagc gaaaaaaatc ccctggatta cagggggaac ccattatcag
      421 actttg
//
LOCUS       DAZGXZ010000464          402 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000464 DAZGXZ010000000
VERSION     DAZGXZ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.467"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            55..333
                     /locus_tag="ACHKJF_005713"
     CDS             55..333
                     /locus_tag="ACHKJF_005713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952841.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQIDELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
BASE COUNT          116 a           79 c          101 g          106 t
ORIGIN      
        1 tacgtcggtt tactcccaat gtgatttgat ggtcaatgag tcaggaataa tcccgtgaaa
       61 aaatgtgtgc cagtcctgct ttttagtgtt ctttctgctt cggttgccag tcatgcggac
      121 agcaatgttt ttcaacaaaa ggccgagaag acaaaaaacg gcattgttgc ggatgttaat
      181 gccagacaga aaatgttcag taacagactg aagatacaac ttcagatcga tgagttgaga
      241 gcagagctgg ctggcaccag agatgcccaa aaaagagagg atttgcgtaa aaaactggag
      301 tctctggagc ggcaaaagaa atccttacgc tgatgggggc gtattttccc cggtttcttc
      361 cataaccttt ttaccgttca gatatttacg ggcgaggcga at
//
LOCUS       DAZGXZ010000465          389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000465 DAZGXZ010000000
VERSION     DAZGXZ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.468"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(159..>389)
                     /locus_tag="ACHKJF_005714"
     CDS             complement(159..>389)
                     /locus_tag="ACHKJF_005714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0952842.1"
                     /translation="AAKAGKLPEAFFWTDAENNDVPVTAEELIALSEAAEQAMFTKGM
                     EIHIRQRTMKKELEKLTSADEILAYRVGWAQE"
BASE COUNT           88 a          119 c           65 g          117 t
ORIGIN      
        1 tgccgctgaa tatttcacat ctgcttacac tgtaatttct gagcaacatc acaatatcat
       61 caaaatttaa ctgtttagtt cagccatgat atttccctgc gtaattatta cgactctttc
      121 agtaaaaaca gaaagggaca atccctcttt tccaccaatt actcctgtgc ccagccaacc
      181 ctgtatgcca ggatctcatc cgcactggtc agcttttcca gctccttctt catggtgcgc
      241 tggcgaatgt ggatttccat ccctttagtg aacatcgcct gctctgccgc ttcactcagc
      301 gcaataagct cttctgctgt caccggcaca tcattgtttt ccgcatccgt ccagaaaaac
      361 gcctccggca gtttccccgc tttcgctgc
//
LOCUS       DAZGXZ010000466          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000466 DAZGXZ010000000
VERSION     DAZGXZ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.469"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          116 a           65 c          122 g           84 t
ORIGIN      
        1 cttcaccggc ataacaaccg gggagaaacg taaaaaataa tcgggaatgt gaccgccagg
       61 agataaacgg ggaggttacg gtggctgagc cgtaccggta tgccgggtgg ttgttacgaa
      121 acgatgcttc cgcacgcgcg cgcgataaag aagggatgat tttcgtgagg aagaggggaa
      181 aactggcgtt ctgataattg cagagttatt tacggggcgg ttacgatggg gcagaccgtg
      241 tccggtgggc aggcgacagg tatcaccgat gggataagac tgtctgattg gtattataaa
      301 tatataagag agatcaaaca catgagatca aatacatgag ttcatatatg tgaaataaac
      361 acggtgagtg tgatcagtgg gcatgat
//
LOCUS       DAZGXZ010000467          385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000467 DAZGXZ010000000
VERSION     DAZGXZ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.470"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..246
                     /locus_tag="ACHKJF_005715"
     CDS             <1..246
                     /locus_tag="ACHKJF_005715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0952843.1"
                     /translation="ASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGN
                     PMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          112 a           75 c          117 g           81 t
ORIGIN      
        1 gccagcatga ttctgggtgg tgtggcccag atgctggccc cgaaggcaaa gacgccggaa
       61 tacaaaagta cggataacgg taaacagaac acgtattttt cgtcactgga caacatgatt
      121 gctcagggta acccgatgcc ggtgccttac ggtgaaatgc tggttggttc acgacggata
      181 tcccaggaca tcagcacccg tgatgagggt ggtgacggga aagtggtggt tatcgggcgg
      241 ggatgaaaat aaaaaaatcc cgcagagtta gcggagctgc gggagagaac gatgaagatt
      301 aactttatgg agttattttt caggcatcaa aaaagtaacg cagcgtcatt attgcggcta
      361 caggcaattg ccggaaatgt gaaga
//
LOCUS       DAZGXZ010000468          383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000468 DAZGXZ010000000
VERSION     DAZGXZ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.471"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            143..264
                     /locus_tag="ACHKJF_005716"
                     /pseudo
     CDS             143..264
                     /locus_tag="ACHKJF_005716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           93 a           82 c           96 g          112 t
ORIGIN      
        1 gtaatcctaa agcttttctt attgactcta agcacaggtc tttgaatttg ttagccagat
       61 ttctgttcta attttggata gcagaatatg caatgttatt tttcgaatcc tccataactg
      121 atatatgtgc tgttagtgga gtacaagaat aagctcgcgt tagcagtctg tcagtactgc
      181 gacagcgttg tctgggaggg ctttacccgg tcaaaacagc attattgtgc accccatgtg
      241 acggatgaac tgtgtgctca gggggcattt tactgtaaaa accgtgctgc ggtcagccgt
      301 aaccgtcagg tgggtgaacg caacagtcgc gccattgctt ttcaggcacg taccctgcag
      361 gcgcatgagc gtcgtatgaa cag
//
LOCUS       DAZGXZ010000469          370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000469 DAZGXZ010000000
VERSION     DAZGXZ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.473"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(21..136)
                     /gene="rrf"
                     /locus_tag="ACHKJF_005717"
     rRNA            complement(21..136)
                     /gene="rrf"
                     /locus_tag="ACHKJF_005717"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(176..251)
                     /locus_tag="ACHKJF_005718"
     tRNA            complement(176..251)
                     /locus_tag="ACHKJF_005718"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(216..218),aa:Thr,seq:ggt)
     gene            complement(266..>370)
                     /gene="rrf"
                     /locus_tag="ACHKJF_005719"
     rRNA            complement(266..>370)
                     /gene="rrf"
                     /locus_tag="ACHKJF_005719"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT           77 a          111 c           92 g           90 t
ORIGIN      
        1 agccttatgt tttatttgat gtctggcagt tccctactct cgcatgggga gaccccacac
       61 taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt gggaccaccg
      121 cgctactgcc gccagacaaa ttcttttcta atctgccgaa ctttaaccta aaaagtggtg
      181 ctgataccca gagtcgaact ggggacctca cccttaccaa gggtgcgctc taccaactga
      241 gccatatcag cacgctaaat ttgatgcctg gcagttccct actctcgcat ggggagaccc
      301 cacactacca tcggcgctac ggcgtttcac ttctgagttc ggcatggggt caggtgggac
      361 caccgcgcta
//
LOCUS       DAZGXZ010000470          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000470 DAZGXZ010000000
VERSION     DAZGXZ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.474"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>362)
                     /locus_tag="ACHKJF_005720"
     CDS             complement(<1..>362)
                     /locus_tag="ACHKJF_005720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0952844.1"
                     /translation="GNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTD
                     RIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRR
                     ERDLTGWMSLSRKPEETW"
BASE COUNT           48 a          120 c          109 g           85 t
ORIGIN      
        1 taccatgtct cctccggttt acgcgacagc gacatccacc ccgtcaggtc acgctcccgc
       61 cgccataccc gtttcgccat tcgccgtccc agcgggtcgt agaggtagcg gctctcgacc
      121 agtggctcgc catgctgtat ccgcgtgtgg aacaccaggc ggtgctggct gtcgtagtgg
      181 tagtggtggg tccgctcgtc gtccgtccgt atcacacccg tcgggatgcg gtccgtcttc
      241 tccgtcagtc tgccgtattc atcatagtgg taaacatagt gcgcatcctc tgcgatgcgg
      301 ttatccggcc acgccgtgag cgtgctgtcc gggtgtagct ccgggtccgg caggcggttt
      361 cc
//
LOCUS       DAZGXZ010000471          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000471 DAZGXZ010000000
VERSION     DAZGXZ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.475"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>356
                     /locus_tag="ACHKJF_005721"
     CDS             <1..>356
                     /locus_tag="ACHKJF_005721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0952845.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZGXZ010000472          348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000472 DAZGXZ010000000
VERSION     DAZGXZ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.476"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>348)
                     /locus_tag="ACHKJF_005722"
     CDS             complement(<1..>348)
                     /locus_tag="ACHKJF_005722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952846.1"
                     /translation="DEVLPAPLPPYRVLTGLVDRFGRTQTFHREAAGEFSGEITGVTD
                     GAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLT
                     HDPEYPENLPAAPL"
BASE COUNT           56 a          117 c          115 g           60 t
ORIGIN      
        1 agcggcgcgg caggtaaatt ctccgggtat tccgggtcgt gcgtcagcca cacggcagac
       61 agacggatgc cgttgtcccg gccatattcg gtgtaacccg gcagggtatc aggaaaagcg
      121 gacggttccg tcccgccgga aatggcctgc tgccgggctt cttctgcccg ctgcgcctgc
      181 gtggtcagta ccagccggaa gtgacgcccg gcaccatccg tcacgccggt gatttcgccg
      241 ctgaattcac cggcggcttc gcggtggaac gtctgtgtgc gcccgaagcg gtccaccagc
      301 ccggtcagta cccggtacgg cggcagcggc gcaggcagca cctcatcc
//
LOCUS       DAZGXZ010000473          346 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000473 DAZGXZ010000000
VERSION     DAZGXZ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.477"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           91 a           48 c           77 g          130 t
ORIGIN      
        1 tgtttttact gagcaaaagc tggcttttaa ttcctgtact gaaacgctgt cgctcgtccg
       61 gggttaactg aaactggatg atgatgtaaa atgaaggctg tttttgtgtt tgtcgaagaa
      121 gtgttatttt aatccattct catcattggc tggcgattat acagaagtat ttgaagaaaa
      181 aatggtgcat tttatctgcg tcagcaggga ggaagtattt tattgatgta tcagtattaa
      241 atggtttttg ttatttataa atatgtcatc ctgtccatag aatattgtac tcagcattca
      301 gtgcaggaac tgtagtcttc tttattttat tcttcttcag gagggg
//
LOCUS       DAZGXZ010000474          340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000474 DAZGXZ010000000
VERSION     DAZGXZ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.478"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT           95 a           54 c          104 g           87 t
ORIGIN      
        1 ataaacacga agacggtgaa cacctttctg tatatgggac ttggtaaaaa cggtcttaag
       61 tacaggagtg cgaagcacct ggttggacgt gcgtagccgg aatgcatatt cgtgagtcgg
      121 gtaatgagag ttgaacggtg gcagtgagaa tggaacagaa aaaaatcaga tgcggaaggc
      181 ggaaatacac agcgggtgcg atgtgcttca tgctgtttgt atggctgggt ggtgtggtga
      241 cattctgtgt gctggccgct ctgataatga ggtgaaaacg gaagagagtt tacttcctta
      301 attcgaactt tctgataact gagaacttgt taaatgctcg
//
LOCUS       DAZGXZ010000475          335 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000475 DAZGXZ010000000
VERSION     DAZGXZ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.479"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>335
                     /locus_tag="ACHKJF_005723"
     CDS             <1..>335
                     /locus_tag="ACHKJF_005723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP0952847.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVY"
BASE COUNT           85 a           76 c           95 g           79 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZGXZ010000476          333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000476 DAZGXZ010000000
VERSION     DAZGXZ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.480"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            32..>333
                     /locus_tag="ACHKJF_005724"
     CDS             32..>333
                     /locus_tag="ACHKJF_005724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFP0952848.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFL"
BASE COUNT           96 a           85 c           76 g           76 t
ORIGIN      
        1 gtcatcgtca tgactcagag aggtaaatac catgattcac ctgttcaaaa cctgcatgat
       61 taccgccttc attctggggt taacgtggtc tgccccactc cgggcacagg atcaacgtta
      121 catcagtata cgcaatacag atacgatatg gctcccggga aatatttgtg cttaccagtt
      181 ccggctggat aatggcggaa acgatgaagg atttggcccc ctcaccatca ctctgcaact
      241 caaagacaaa tatggtcaga cgctggtgac cagaaaaatg gaaacggaag cctttggtga
      301 cagtaatgcc acgcgaacca cagacgcatt tct
//
LOCUS       DAZGXZ010000477          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000477 DAZGXZ010000000
VERSION     DAZGXZ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.481"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>324
                     /locus_tag="ACHKJF_005725"
     CDS             <1..>324
                     /locus_tag="ACHKJF_005725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952849.1"
                     /translation="NYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFR
                     DTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQK
                     LLDGI"
BASE COUNT          128 a           51 c           62 g           83 t
ORIGIN      
        1 aaaattataa ccgtttgttt ttaccaaatt caaaaaacca aacaaataat cgtttcaaaa
       61 cgttaaacag caaagctatt ggcaggctac ttgctgctgg tggcgtatac aatgggaaca
      121 ttgaaggatt cagagatact gcggaaaaac tgggtggaga tgcaataaaa ggctatgatc
      181 aaatactaaa tgaaaaaaca gcgggcatag cgatagcaac agcatctatt cttttaacaa
      241 agcgttctaa tgttgataca tatacagaaa taaatagtta cttaggcaaa cttagaggtc
      301 aacaaaaact tcttgatggt atag
//
LOCUS       DAZGXZ010000478          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000478 DAZGXZ010000000
VERSION     DAZGXZ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.482"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..191)
                     /locus_tag="ACHKJF_005726"
     CDS             complement(<1..191)
                     /locus_tag="ACHKJF_005726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0952850.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTYFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFV"
     gene            complement(181..>323)
                     /locus_tag="ACHKJF_005727"
     CDS             complement(181..>323)
                     /locus_tag="ACHKJF_005727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP0952851.1"
                     /translation="SLLPESEKNIEMRVNGALTGYGELVEVDDKLGVEIHSWLSGHNN
                     VK"
BASE COUNT          114 a           69 c           51 g           89 t
ORIGIN      
        1 attacaaaca ttgacaatag caaagcaacc ccattaagtg tcatatttga tggcacttgc
       61 tgaagcccca gcgcgtttcg gacaataaca aaaacaatcg agaatttaat aaaatatgtc
      121 ccggatgcta ttataaacgg caatagtgta aataatgata gtatagctat cagcgaaata
      181 ctatttgaca ttattatgcc ctgataacca agaatgaatc tctacgccca atttatcatc
      241 cacttcaaca agttcgccat agccagttag tgcgccattt acacgcattt ctatattttt
      301 ctcagattca ggaagcagag aca
//
LOCUS       DAZGXZ010000479          323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000479 DAZGXZ010000000
VERSION     DAZGXZ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.483"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          100 a           94 c           52 g           77 t
ORIGIN      
        1 ggaattttgc ttatccacat taaactgcaa gggacttccc cataaggtta caaccgttca
       61 tctcataaag cgccagccgc cagcgttaca gggtgcaatg tatcttttaa acatctgttt
      121 atatatcctt taaactactt aattacattc atttaaaaag aaaacctatt cattgcctgt
      181 cctgtggaca gacagatatg cacctcccac cgcaagcggc gggccccgac cggagctact
      241 ttagttacaa cactcagaca taaccaccag aaaaaccccg gtccagcgca gaactgaaac
      301 cacaaagccc ctccctcata act
//
LOCUS       DAZGXZ010000480          322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000480 DAZGXZ010000000
VERSION     DAZGXZ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.484"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          100 a           79 c           66 g           77 t
ORIGIN      
        1 ggaaggccac acacaaaaat atcatatgag tgacaatgct gactggccga agccccgcga
       61 agcgggaacc catcttatat atacccggta actgcgcagc ctcctgccag accatttttc
      121 gcgggcacaa tatttacaga caaccggaaa gccgattgat ggaattgagg tatatcagac
      181 gacacagcga tgccgaagaa tgataatata agtaaagccc cgaaaaattt tcggggcttt
      241 acttatatta tcatgacttc cactttctga actgttcggc aatcacccat aacgccttgt
      301 tgattcggat attaccatca at
//
LOCUS       DAZGXZ010000481          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000481 DAZGXZ010000000
VERSION     DAZGXZ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.485"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..240)
                     /locus_tag="ACHKJF_005728"
     CDS             complement(<1..240)
                     /locus_tag="ACHKJF_005728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HFP0952852.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPD"
BASE COUNT          101 a           68 c           52 g           99 t
ORIGIN      
        1 atcgggtgca ccgttatcga atgtcatttg catttgaaaa cgggaattaa aaaagccttt
       61 gtcaaaattt tgataactta acgtcatatc gtaggcatgt tcacctttct ttttagcatc
      121 gttaaaatct ttaataaatt tttcgacacc aggctgaatt tgtgtacctg tgtaccatgt
      181 gccaccaccc cagattacgg ctaacgcaac aataacacct gtagctgact tacgtatcat
      241 ataaatattc cctgataaaa cagcaaaact ttgaatatcc acttatgctg aaataatgtc
      301 cagcggcagg atgcattatc
//
LOCUS       DAZGXZ010000482          320 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000482 DAZGXZ010000000
VERSION     DAZGXZ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.486"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            77..>320
                     /locus_tag="ACHKJF_005729"
     CDS             77..>320
                     /locus_tag="ACHKJF_005729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HFP0952853.1"
                     /translation="MRKISVSYICKSLFLYCSASLPVLSANGIPDKMIVELGDNSAAK
                     ANFAVAVGQYSQANAEKSISLGASARAIENNSTALGA"
BASE COUNT          100 a           51 c           60 g          109 t
ORIGIN      
        1 ttttacttgt atataaatat ccaacgatgg ttctgtttgt attatgtata ctatataaaa
       61 gagatggtga attcaaatgc gtaaaatcag tgtcagttat atatgcaaat cattatttct
      121 ctactgctca gcttctttac ctgtcttatc tgcaaatggt attcctgata aaatgatcgt
      181 ggaacttggt gataacagtg cggcaaaagc taatttcgct gttgctgttg gtcaatattc
      241 acaggcaaat gcagaaaaaa gtatttcttt aggtgcatca gcaagagcta ttgaaaataa
      301 ctctaccgcg ttaggtgcaa
//
LOCUS       DAZGXZ010000483          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000483 DAZGXZ010000000
VERSION     DAZGXZ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.487"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..271
                     /locus_tag="ACHKJF_005730"
     CDS             <1..271
                     /locus_tag="ACHKJF_005730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP0952854.1"
                     /translation="VFRGCLFNSFKFWFNDNIYISAIVTSVIFSALHLQYTDFRTFLM
                     LFLISLVLISAKIKSNGLLMPILLHMAMNAVITGIQYTLQYHITV"
BASE COUNT          101 a           54 c           48 g          116 t
ORIGIN      
        1 tgtatttcga gggtgtcttt ttaactcttt taaattctgg tttaatgaca atatttacat
       61 ctctgctatt gtaacatcag tcatattttc tgcattacat ctacaatata ctgactttcg
      121 tacatttttg atgctctttc tgatatcgtt agttcttatc agtgcaaaaa taaagtcgaa
      181 tggcctgtta atgcccatat tacttcacat ggcgatgaat gcagtcataa caggaataca
      241 atatacgtta caatatcaca taacagtctg aaattgttat tatgagatat ctataacacc
      301 tgaagttgga aaaagaagc
//
LOCUS       DAZGXZ010000484          314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000484 DAZGXZ010000000
VERSION     DAZGXZ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.488"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..296)
                     /locus_tag="ACHKJF_005731"
     CDS             complement(<1..296)
                     /locus_tag="ACHKJF_005731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFP0952855.1"
                     /translation="MVIETLNTNEGCFFVWRFIESNDKNISTSLISKSNDGLMFFSGE
                     YVDIEPYLVGFSHYYSAAVTKISISKSKKKTMNFFKMNGFSSRDHWLDEMLRSE"
BASE COUNT          108 a           64 c           41 g          101 t
ORIGIN      
        1 ttttcagacc tgagcatttc atccaaccag tgatctctgg aactaaaccc attcattttg
       61 aaaaaattca tcgttttctt cttggattta gatatactaa tttttgtcac tgctgcagaa
      121 taataatgac taaaccctac aagatagggt tcaatatcta catattcccc agaaaaaaac
      181 atcaacccat cattagattt agaaatcaaa gatgttgaaa tatttttgtc atttgactca
      241 ataaatcgcc agacgaaaaa acaaccttcg tttgtattga gtgtttcgat aaccatgctc
      301 catgacatag cact
//
LOCUS       DAZGXZ010000485          311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000485 DAZGXZ010000000
VERSION     DAZGXZ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.489"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..271)
                     /locus_tag="ACHKJF_005732"
     CDS             complement(<1..271)
                     /locus_tag="ACHKJF_005732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFP0952856.1"
                     /translation="MKRILSFCFLFSVHNVFASPLPALLRDALIHDPSVMEARAEATS
                     ARSRVEQARSAHWPVVTATGSKLLSQSHRYDYDYDSEDILPGIRGE"
BASE COUNT           84 a           76 c           89 g           62 t
ORIGIN      
        1 tttcaccgcg gataccgggc aaaatatctt cagagtcgta gtcatagtcg tagcggtgac
       61 tctgggaaag gagtttactg cctgttgctg tgacgaccgg ccagtgtgct gaacgagcct
      121 gctccacacg ggaacgggca gaggtggctt ctgcccttgc ctccatcact gatggatcat
      181 gaatgagagc atccctcagg agggccggca gtggagaagc gaacacgttg tggacagaga
      241 aaagaaaaca gaaagataat attctcttca cccgaacctc cgggacacgc gggaaatata
      301 atcaggcatc a
//
LOCUS       DAZGXZ010000486          309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000486 DAZGXZ010000000
VERSION     DAZGXZ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.490"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>309
                     /locus_tag="ACHKJF_005733"
     CDS             <1..>309
                     /locus_tag="ACHKJF_005733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952857.1"
                     /translation="TVTSKTDGVADAPALTIPEVADSVANAAELKDGLQAEVKLPAGT
                     VEGAVITLTVTHPDKTTRTETHTVSKDEAADGTVSMVVPKGSVVDGQNSVSVSLTQGS
                     "
BASE COUNT           84 a           64 c           95 g           66 t
ORIGIN      
        1 cactgtgacg tcaaaaactg atggtgtggc agatgctccg gccctgacca ttccagaagt
       61 ggctgatagt gtggccaatg cggctgagct gaaagatggt ttacaggcag aagtcaagtt
      121 gccggcagga acagtggaag gtgctgtcat tacactgaca gtgactcatc cggataaaac
      181 caccagaacc gagacgcata ctgttagcaa agatgaggct gcagacggta ctgtgtcaat
      241 ggttgttccg aagggaagtg ttgttgacgg tcagaacagt gtgagtgtca gcctgactca
      301 gggcagtaa
//
LOCUS       DAZGXZ010000487          309 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000487 DAZGXZ010000000
VERSION     DAZGXZ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.491"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            30..>309
                     /locus_tag="ACHKJF_005734"
     CDS             30..>309
                     /locus_tag="ACHKJF_005734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP0952858.1"
                     /translation="MNTKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GVGIWTICRGATRVDGNPVIPGMKLSKEKCDQVNAIERDKALAWVEKNI"
BASE COUNT           83 a           70 c           90 g           66 t
ORIGIN      
        1 gggacagcgg ctgaatattt aatatatcca tgaacacaaa aatcaaatac ggcctgtcgg
       61 ctgccgttct ggcgttgatt gccgcagggg cttctgcgcc tgaaatcctc gaccagtttc
      121 tggatgaaaa agaaggtaac cacaccacgg cataccgtga tggtgtgggt atctggacca
      181 tctgccgtgg agccacccgg gtggatggta atcctgtgat tcctggcatg aagctgtcga
      241 aggaaaaatg cgaccaggtt aacgccattg aacgggataa ggcgctggca tgggtggaga
      301 aaaacatca
//
LOCUS       DAZGXZ010000488          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000488 DAZGXZ010000000
VERSION     DAZGXZ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.492"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>306
                     /locus_tag="ACHKJF_005735"
     CDS             <1..>306
                     /locus_tag="ACHKJF_005735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952859.1"
                     /translation="TAKQNADTLKGEADSAVKALPDTAAEKGGLQGRVDALDGIQVPE
                     VNDQDGNGKADAEEAAAAVAAATEKVEAAEAADKAAKDKLAELNADKLITPEEKAQL"
BASE COUNT           84 a           67 c          122 g           33 t
ORIGIN      
        1 aaacggcgaa gcagaatgct gacaccctga agggtgaggc agacagtgcc gtgaaggcgt
       61 tgccggatac agccgcggag aaaggtggcc tgcagggtcg tgtggatgca ctggatggta
      121 tccaggtacc ggaagtgaac gatcaggacg gcaacggcaa ggcggatgca gaggaagcgg
      181 cagcagcggt ggcggcggcc acggagaaag tggaagcggc agaagcggcg gacaaggctg
      241 cgaaggacaa gcttgcagag ctgaatgcag acaagctgat cacgccggaa gagaaggcgc
      301 agctgg
//
LOCUS       DAZGXZ010000489          306 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000489 DAZGXZ010000000
VERSION     DAZGXZ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.493"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>306
                     /locus_tag="ACHKJF_005736"
     CDS             <1..>306
                     /locus_tag="ACHKJF_005736"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP0952860.1"
                     /translation="HMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWIGND
                     VVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIKWW"
BASE COUNT           97 a           43 c           71 g           95 t
ORIGIN      
        1 tcacatgatg acttataacg gtgaatttga taagtttgct atgagtgaat ttgaacgtag
       61 ctggacattg aaaccattca ttaccaaacc tgaaaatccg attataggaa atgatgtctg
      121 gataggaaat gatgttgtat tgaaaggtgg catcgcaata ggtgatggtg ctgttattgc
      181 tgccaattcc gttgttacca aagatgtgcc cccatatgcc atagttgcag gagttcctgc
      241 aaaaataatc agatttaggt ttgattcaaa tgttattgat gaactgttga gaataaaatg
      301 gtggaa
//
LOCUS       DAZGXZ010000490          305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000490 DAZGXZ010000000
VERSION     DAZGXZ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.494"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..217
                     /locus_tag="ACHKJF_005737"
     CDS             <1..217
                     /locus_tag="ACHKJF_005737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0952861.1"
                     /translation="HVSLRRALYMPAMVATSKTEWGRAFRDRLAANGKKGKVILGAMM
                     RKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT           75 a           71 c          104 g           55 t
ORIGIN      
        1 acatgtgtcg cttcgcaggg cgttgtatat gcccgcaatg gtagccacca gtaagactga
       61 gtggggacgg gcgttccgcg accgtctggc ggctaatggc aagaaaggaa aggtgattct
      121 cggcgcgatg atgcgcaagc tggcacaggt ggcgtatgga gtgctgaagt caggcgtgcc
      181 gttcgatgcg tcacggcata atccggtagc ggcgtaaaaa tcgcggaagg gatgaaaaaa
      241 acagcgcctg acggcgctgt gtctggcatg cctgcaatcc gggaaaccgg accaggaaaa
      301 aactt
//
LOCUS       DAZGXZ010000491          299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000491 DAZGXZ010000000
VERSION     DAZGXZ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.495"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..242)
                     /locus_tag="ACHKJF_005738"
     CDS             complement(<1..242)
                     /locus_tag="ACHKJF_005738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0952862.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           84 a           62 c           68 g           85 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgg
//
LOCUS       DAZGXZ010000492          294 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000492 DAZGXZ010000000
VERSION     DAZGXZ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.496"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
BASE COUNT          105 a           55 c           52 g           82 t
ORIGIN      
        1 aataatgaaa gttaataaca taaaaaagag gttaccgcct tataatgata agagttcatt
       61 atccattcca tcccgacaaa acctgcctgt gtataaaaaa taaggaccat gtcacaaaac
      121 attacattcc ggcaagttat gacagaatta ctgaaaaccg ttatctgtag taattgtagt
      181 ttctgatata tgctcatcca tccggaaata cgacatggtc ctgactggcg ttcacttcaa
      241 agtgtaattt attattcaca cactgagact gaaaaacgga tggcgtggat gagt
//
LOCUS       DAZGXZ010000493          287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000493 DAZGXZ010000000
VERSION     DAZGXZ010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.497"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..>287)
                     /locus_tag="ACHKJF_005739"
     CDS             complement(<1..>287)
                     /locus_tag="ACHKJF_005739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952863.1"
                     /translation="ANVGIPADKVTQDGEYTVKAEITDPAGNTSGQGKATQFGVDTVA
                     PSEPALKAENDGSVSATLPDGANKGDKVEVTFTDEEGNEQKVTLEKGDGNW"
BASE COUNT           52 a           95 c           55 g           85 t
ORIGIN      
        1 ctccagttgc catccccttt ctccagcgtg actttctgct cattaccctc ttcatccgta
       61 aaggtcactt ccaccttgtc acctttgttc gcaccatctg gcagagtcgc tgatacagaa
      121 ccatcattct cagccttcag tgctggttct gacggagcta cagtatccac gccaaactgg
      181 gtagctttac cctgaccact ggtgttgcct gccgggtctg tgatttcagc cttaactgtg
      241 tattccccat cctgcgtcac tttatccgca ggaatgccca cattcgc
//
LOCUS       DAZGXZ010000494          285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000494 DAZGXZ010000000
VERSION     DAZGXZ010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.499"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..253
                     /locus_tag="ACHKJF_005740"
     CDS             <1..253
                     /locus_tag="ACHKJF_005740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952864.1"
                     /translation="PYCHHASVRVDEKERQVRCKICGAVVEPFDWMPSVAKRETRLAD
                     DVRLLRQEERERRRNVEKLIQIERNAKARIRRAAKSRTE"
BASE COUNT           90 a           55 c           79 g           61 t
ORIGIN      
        1 accatattgt catcacgcct ctgtccgggt tgacgaaaaa gagcgtcagg tccgctgtaa
       61 aatctgcggt gcagttgtgg agccatttga ctggatgccc tctgtggcga aaagagaaac
      121 cagactggca gatgatgtaa ggctcttgcg ccaggaggaa cgggaaaggc ggagaaatgt
      181 agaaaagcta attcagattg agcgtaacgc gaaagcgcgg atacgcaggg cggcaaaatc
      241 aagaactgaa taattaaatt tagcactgtt aaaaatttaa tcctt
//
LOCUS       DAZGXZ010000495          284 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000495 DAZGXZ010000000
VERSION     DAZGXZ010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.500"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..234)
                     /locus_tag="ACHKJF_005741"
     CDS             complement(<1..234)
                     /locus_tag="ACHKJF_005741"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952865.1"
                     /translation="MKHLESSGSALYFSRPGGEFHTLTAGSMDISDSVLVMRTDLNHS
                     DQLRVTESLRGKNNLLLVDFTERSDGQKALNIPL"
BASE COUNT           68 a           89 c           62 g           65 t
ORIGIN      
        1 cagagggata ttcagcgcct tctgcccgtc agaacgctca gtaaaatcaa ccaacaacag
       61 gttattctta ccccgcagcg attctgtcac ccgaagctgg tctgagtggt taaggtccgt
      121 ccgcatcacc agaacactgt cgctgatatc catactgcct gccgtcaggg tatggaactc
      181 cccgcccggc ctgctgaaat acagagcact tcccgatgac tcaagatgct tcacggatga
      241 gtttcccgtc atcatccagg ccgcattctg cagattcaca ttac
//
LOCUS       DAZGXZ010000496          280 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000496 DAZGXZ010000000
VERSION     DAZGXZ010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.501"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..>280
                     /locus_tag="ACHKJF_005742"
     CDS             <1..>280
                     /locus_tag="ACHKJF_005742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952866.1"
                     /translation="AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSIHGVQTRECG
                     HTSRSPLDTLESAIGFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSG"
BASE COUNT           58 a           84 c           82 g           56 t
ORIGIN      
        1 ttgcggccct gttaccggag ggatggtcac gggacatgac gacgttcctt tcactcagtc
       61 aggaggtgct gttatccctg ctcagtttct gcaccgcgtg cagcatccac ggcgttcaga
      121 cccgcgagtg tggtcacacg tcacgcagcc cgctggacac gctggagagc gccatcggtt
      181 ttcacatgcg cgactggtgg cagccgacaa aagcaaactt cttcggacac ctgaaaaagc
      241 cgcagattat cgcagccctg aatgaggcag gactgtccgg
//
LOCUS       DAZGXZ010000497          278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000497 DAZGXZ010000000
VERSION     DAZGXZ010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.502"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            6..197
                     /locus_tag="ACHKJF_005743"
     CDS             6..197
                     /locus_tag="ACHKJF_005743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952867.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
BASE COUNT           62 a           72 c           93 g           51 t
ORIGIN      
        1 gggtaatgaa tatcagcacg gaaacccgcg aaattctgcg taattacagg gccgtgatta
       61 atgcgcggcg tcgtgaaatg gggcagaaac cgctcaccac cgcgcagatt gttgatgaaa
      121 tctgcgattt tgtggcgaat cagcaggcgg ttttcctcgg tggtcactac atccttcagg
      181 gcagcaggca caggtgatac agggcaggcg gtggaggccg cctgtcagcc cgcgcgaaaa
      241 cccctggcag gctggcgcct gccggaaggg atgcgggc
//
LOCUS       DAZGXZ010000498          276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000498 DAZGXZ010000000
VERSION     DAZGXZ010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.503"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            <1..196
                     /locus_tag="ACHKJF_005744"
     CDS             <1..196
                     /locus_tag="ACHKJF_005744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0952868.1"
                     /translation="PAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKG
                     AQDELRLQLRDGGMLSGSGR"
     gene            193..>276
                     /locus_tag="ACHKJF_005745"
     CDS             193..>276
                     /locus_tag="ACHKJF_005745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0952869.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDG"
BASE COUNT           63 a           55 c          104 g           54 t
ORIGIN      
        1 tccggcgcag atgcggcggg cggaaggtat taattttaat cagaacaatc acgtggtgat
       61 tcagaacgac ggtatcaacg gacaggcggg gccgcagctg atgaaggcgg tgtatgacat
      121 ggcccgcaag ggggcgcagg atgaactccg gctgcagttg cgtgatggcg gtatgttatc
      181 agggagcggg cgatgaaaac ctttcgctgg aaagtgaagc cggatatgga ggtgaactcg
      241 cagccgtcgg tgcgtgaagt gcgttttggt gacggg
//
LOCUS       DAZGXZ010000499          274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000499 DAZGXZ010000000
VERSION     DAZGXZ010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.504"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..126)
                     /locus_tag="ACHKJF_005746"
     CDS             complement(<1..126)
                     /locus_tag="ACHKJF_005746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952870.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRHIAQDWSL"
     gene            complement(173..>274)
                     /locus_tag="ACHKJF_005747"
     CDS             complement(173..>274)
                     /locus_tag="ACHKJF_005747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0952871.1"
                     /translation="DAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
BASE COUNT           58 a           84 c           67 g           65 t
ORIGIN      
        1 cagactccag tcctgggcta tgtggcgcac atccccacgg gtcagcacca cggcaaaaac
       61 gcattcactg tccggcacgt ctgtatccag tacatcgcgg atgatttctt taacagtaca
      121 gtacatattc tcgtctcctt tacaggggcg gcatcgccgc cccgtccgtt aatcagtcca
      181 gtgctgcgta aatcgcgtta cattcggggt gaaattcgat gtggctccac aactgcgcat
      241 cgcgcatccg gtaaaggtgt gtcagtcccg catc
//
LOCUS       DAZGXZ010000500          274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000500 DAZGXZ010000000
VERSION     DAZGXZ010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.denovo.505"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..126)
                     /locus_tag="ACHKJF_005748"
     CDS             complement(<1..126)
                     /locus_tag="ACHKJF_005748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0952872.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIARDWNL"
     gene            complement(173..>274)
                     /locus_tag="ACHKJF_005749"
     CDS             complement(173..>274)
                     /locus_tag="ACHKJF_005749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0952873.1"
                     /translation="DAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
BASE COUNT           54 a           85 c           69 g           66 t
ORIGIN      
        1 caggttccag tcccgggcta tgtggcgcac atccccgcgg gtcagcacca cggcaaaaac
       61 acattcactg tccggcacgt ctgtattcag cacttcgcgg ataatttctt taacagtaca
      121 gtacatgttc tcgtctcctt tacaggggcg gcattgccgc cccgtccgtt aatcagtcca
      181 gtgccgcgta aatcgcgttg cattcggggt gaaattcgat gtggctccac aactgcgcat
      241 cgcgcatccg gtacaggtgt gtcagtcccg catc
//
LOCUS       DAZGXZ010000501         6834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.guided.213, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000501 DAZGXZ010000000
VERSION     DAZGXZ010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.guided.213"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..200)
                     /locus_tag="ACHKJF_005750"
     CDS             complement(<1..200)
                     /locus_tag="ACHKJF_005750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0952874.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRK"
     gene            complement(419..1396)
                     /gene="epmA"
                     /locus_tag="ACHKJF_005751"
     CDS             complement(419..1396)
                     /gene="epmA"
                     /locus_tag="ACHKJF_005751"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0952875.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1721..3529
                     /gene="frdA"
                     /locus_tag="ACHKJF_005752"
     CDS             1721..3529
                     /gene="frdA"
                     /locus_tag="ACHKJF_005752"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0952876.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3522..4256
                     /gene="frdB"
                     /locus_tag="ACHKJF_005753"
     CDS             3522..4256
                     /gene="frdB"
                     /locus_tag="ACHKJF_005753"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0952877.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4267..4662
                     /gene="frdC"
                     /locus_tag="ACHKJF_005754"
     CDS             4267..4662
                     /gene="frdC"
                     /locus_tag="ACHKJF_005754"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0952878.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4673..5032
                     /gene="frdD"
                     /locus_tag="ACHKJF_005755"
     CDS             4673..5032
                     /gene="frdD"
                     /locus_tag="ACHKJF_005755"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0952879.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5095..6228
                     /locus_tag="ACHKJF_005756"
     CDS             5095..6228
                     /locus_tag="ACHKJF_005756"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0952880.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6317..>6834
                     /gene="blc"
                     /locus_tag="ACHKJF_005757"
     CDS             6317..>6834
                     /gene="blc"
                     /locus_tag="ACHKJF_005757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0952881.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWV"
BASE COUNT         1720 a         1767 c         1772 g         1575 t
ORIGIN      
        1 tctttgcgat aagcagctcc catttcagcc atcattaagg cgaatggaat aaagaataac
       61 aatgcagaaa atatataaaa gggaatcgca ctataaccca ttaagtaata agccgatggg
      121 ctattggcaa atccaaatac ggaagtaaag atcatcaata tgagtcctat cagactcatc
      181 ttttttatcg tgtgaggcat gaaaccatcc ttcatttcag cgcgtgtata acacgtgccc
      241 actgaactgc actgcgcaac attgagcagg cagcgtaaaa accaatacag aattgatacc
      301 tggatgatat caaatatcca gtcttaaatt gtggccttta acggaaaaat gaaaacatat
      361 gctacagaat ggcgcttatc acgccattct tcgctgttaa ttcagtaatt tttcagaatt
      421 atgcccggtc aacgctaaag gcgatgactt cagccagtgt ctccgcgccc agcgccaaca
      481 tcaccagacg atcaacacct aatgccacgc cggaacagtc aggcataccg actttcaagg
      541 cttcaatcag attctggtca atggggtgct gcggcaaacc gcgcgccgcg cgcttacggt
      601 tatcttgttc aaagcgttgt tgctgctcac gggcatccgt caattcatgg aaaccattcg
      661 ccagctcaat acctttataa taaacctcaa agcgttcagc gacccgatga tcttcggtac
      721 tgatttgcgc cagtgatgcc tggctggctg gaaagtggta cacaaaggtc ggtttttctt
      781 tgccaatatt tggctctacg ccaaaggtaa acagcaattg tagcagcgtg tcgcggtctt
      841 cttcggtatc agcaacattg ctcaaatcca gtttcgctgc gacttcccgc agttgcgttt
      901 tgtcggcaga gagcgggtca atttccagat aacgcaagaa agcttgttga taagaaaggc
      961 tttctgctgc cgggcagtcc agcacctgtt gtaagagatc gtccacctcg ttcatcaacc
     1021 ggtacatatc atagtgcggt cgataccact ccagcatagt gaactcaggg ttgtgataac
     1081 gccccatctc ttcattacgg aagctgcggc acagctggaa taccggccca caaccggcaa
     1141 ccagcaggcg tttcatatgg tattccgggc tggtcattaa ccagagattc atcccctgcg
     1201 aatgcccggg gccaacgaaa cgtgtctcaa acgggaccaa atgaatatcg gttaccgtcg
     1261 cctggctcat acaaggcgtc tccacctcca gcactccacg atcggcaaag aaacgacgga
     1321 tctccgccat aatcgccgcg cgttttaata agttaggaat ggatgcgctc ggctgccagg
     1381 atgccgtttc gctcatagtt aaatctccag tttttgacaa gggcacgaag tctactcgca
     1441 acgcgacggc gagacaaatt ttacgcagga atcaaacagc ggtgggcagt gactaaaaaa
     1501 agcacgatct gatggtttag taattaaatt aatcatcttc agtgataatt tagccctctt
     1561 gcgcactaaa aaaatcgatc tcgtcaaatt tcagacttat ccatcagact atactgttgt
     1621 acctataaag gagcagtgga atagcgttcg cagaccgtaa ctttcaggca cttaccctga
     1681 agtacggggc tgtgggataa aaacaatctg gaggaatgtc gtgcaaacct ttcaagccga
     1741 tcttgccatt gtaggcgccg gtggcgcggg attacgtgct gcaattgctg ccgcgcaggc
     1801 aaatccaaat gcaaaaatcg cactaatctc aaaagtatac ccgatgcgta gccataccgt
     1861 tgctgcagaa gggggctccg ccgctgtcgc gcaggatcat gacagcttcg aatatcactt
     1921 tcacgataca gtagcgggtg gcgactggtt gtgtgagcag gatgtcgtgg attatttcgt
     1981 ccaccactgc ccaaccgaaa tgacccaact ggaactgtgg gggtgcccat ggagccgtcg
     2041 cccggatggt agcgtcaacg tacgtcgctt cggcggcatg aaaatcgagc gcacctggtt
     2101 cgccgccgat aagaccggct tccatatgct gcacacgctg ttccagacct ctctgcaatt
     2161 cccgcagatc cagcgttttg acgaacattt cgtgctggat attctggttg atgatggtca
     2221 tgttcgcggc ctggtagcaa tgaacatgat ggaaggcacg ctggtgcaga tccgtgctaa
     2281 cgcggtcgtt atggctactg gcggtgcggg tcgcgtttat cgttacaaca ccaacggcgg
     2341 catcgttacc ggtgacggta tgggtatggc gctaagccac ggcgttccgc tgcgtgacat
     2401 ggaattcgtt cagtatcacc caaccggtct gccaggttcc ggtatcctga tgaccgaagg
     2461 ctgccgcggt gaaggcggta ttctggtcaa caaaaatggc taccgttatc tgcaagatta
     2521 cggcatgggc ccggaaactc cgctgggcga gccgaaaaac aaatatatgg aactgggtcc
     2581 acgcgacaaa gtttctcagg ccttctggca cgaatggcgt aaaggcaaca ccatctccac
     2641 gccgcgtggc gatgtggttt atctcgacct gcgtcacctc ggcgagaaaa aactgcatga
     2701 acgtctgccg ttcatctgcg aactggcgaa agcgtacgtt ggcgtcgatc cggttaaaga
     2761 accgattccg gtacgtccga ccgcacacta caccatgggc ggtatcgaaa ccgatcagaa
     2821 ctgtgaaacc cgcattaaag gtctgttcgc cgtgggtgaa tgttcctctg ttggtctgca
     2881 cggtgcaaac cgtctgggtt ctaactccct ggcggaactg gtggtcttcg gccgtctggc
     2941 cggtgaacaa gcgacagagc gtgcagcaac tgccggtaat ggcaacgaag cggcaattga
     3001 agcgcaggca gctggcgttg aacaacgtct gaaagatctg gttaaccagg atggcggcga
     3061 aaactgggcg aagatccgcg acgaaatggg cctggcaatg gaagaaggtt gcggtatcta
     3121 ccgtacgccg gaactgatgc agaaaaccat cgacaagctg gcagagctgc aggaacgctt
     3181 caagcgcgtg cgcatcaccg acacctccag cgtgttcaac accgacctgc tctacaccat
     3241 tgaactgggc cacggtctga acgttgctga atgtatggcg cactccgcaa tggcacgtaa
     3301 agagtcccgc ggcgcacacc agcgtctgga cgaaggttgc accgagcgtg acgacgtcaa
     3361 cttcctcaaa cacaccctcg ccttccgcga tgctgatggc acgactcgcc tggagtacag
     3421 cgacgtgaag attactacgc tgccgccagc taaacgcgtt tacggtggcg aagcggatgc
     3481 agccgataag gcggaagcag ccaataagaa ggagaaggcg aatggctgag atgaaaaacc
     3541 tgaaaattga ggtggtgcgc tataacccgg aagtcgatac cgcaccgcat agcgcattct
     3601 atgaagtgcc ttatgacgca actacctcat tactggatgc gctgggctac atcaaagaca
     3661 acctggcacc ggacctgagt taccgctggt cctgccgtat ggcgatttgt ggttcctgcg
     3721 gcatgatggt taacaacgtg ccaaaactgg catgtaaaac cttcctgcgt gattacaccg
     3781 acggtatgaa ggttgaagcg ttagctaact tcccgattga acgcgatctg gtggtcgata
     3841 tgacccactt catcgaaagt ctggaagcga tcaaaccgta catcatcggc aactcccgca
     3901 ccgcggatca gggtactaac atccagaccc cggcgcagat ggcaaagtat caccagttct
     3961 ccggttgcat caactgtggt ctgtgctacg ccgcgtgccc gcagtttggc ctgaacccag
     4021 agttcatcgg tccggctgcc attacgctgg cgcatcgtta taacgaagat agccgcgacc
     4081 acggtaagaa ggagcgtatg gcgcagttga acagccagaa cggcgtatgg agctgtactt
     4141 tcgtgggcta ttgctccgaa gtctgcccga aacacgtcga tccggctgcg gccattcagc
     4201 agggcaaagt agaaagttcg aaagactttc ttatcgcgac cctgaaacca cgctaaggag
     4261 tgcaacatga cgactaaacg taaaccgtat gtacggccaa tgacgtccac ctggtggaaa
     4321 aaattgccgt tttatcgctt ttacatgctg cgcgaaggca cggcggttcc ggctgtgtgg
     4381 ttcagcattg aactgatttt cgggctgttt gccctgaaaa atggcccgga agcctgggcg
     4441 ggattcgtcg actttttaca aaacccggtt atcgtgatca ttaacctgat cactctggcg
     4501 gcagctctgc tgcacaccaa aacctggttt gaactggcac cgaaagcggc caatatcatt
     4561 gtaaaagacg aaaaaatggg accagagcca attatcaaaa gtctctgggc ggtaactgtg
     4621 gttgccacca tcgtaatcct gtttgttgcc ctgtactggt aaggagcctg agatgattaa
     4681 tccaaatcca aagcgttctg acgaaccggt attctggggc ctcttcgggg ccggtggtat
     4741 gtggagcgca attattgcgc cggtgatgat cctgctggtg ggtattctgc tgccactggg
     4801 gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg gcgttcgcgc agagcttcat
     4861 tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg tggtgtggtt tacaccgtat
     4921 gcaccacgcg atgcacgatc tgaaaattca cgtgcctgcg ggcaaatggg ttttctacgg
     4981 tctggctgct atcctgacag ttgtcacgct gattggtatc gttacaatct aacgtatcgc
     5041 caatgtaaat ccggcccgcc tatggcgggc cgttttgtat ggaaaccaga ccttatgttc
     5101 aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc tgcccctcaa
     5161 caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca aaagatcccc
     5221 ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg gggctatgcg
     5281 gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg ttcggtcagc
     5341 aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat caagttaagc
     5401 gatcccacaa caaaatactg gcctgaactt accgctaaac agtggaatgg gatcacacta
     5461 ttacatctcg caacctacac tgctggcggc ctgccattgc aggtgccgga tgaggtgaaa
     5521 tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc gccaggaaca
     5581 caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt gaagccgtct
     5641 ggtttgagtt ttgagcaggc gatgcaaaca cgtgtcttcc agccactcaa actcaaccat
     5701 acgtggatta atgtaccgcc cgcagaagaa aagaattacg cctggggata tcgcgaaggc
     5761 aaggcagttc atgtttcgcc aggggcgtta gatgctgaaa cttatggtgt gaagtcgacc
     5821 attgaagata tggcctgctg ggtacgaagc aatatgaatc cccgtgatat caacgacaaa
     5881 acacttcagc aagggataca actggcacaa tctcgctact ggcaaaccgg cgatatgtat
     5941 cagggcctgg gctgggaaat gctggactgg ccggtaaatc ctgacagcat cattaacggc
     6001 agtggcaata aaattgcact ggcagcacac cccgtaaaag cgattacgcc cccaactcct
     6061 gcagtacgcg catcatgggt acataaaaca ggggcaaccg gcggatttgg tagctatgtc
     6121 gcgtttattc cagaaaaaga gctgggtata gtgatgctgg ctaacaaaaa ctatcccaat
     6181 ccagcgagag tcgccgccgc ctggcagatt ctcaacgctc tacagtaaaa tttcatcggg
     6241 tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt agaaaaaaac
     6301 cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag ctgcatttct
     6361 ggtcgttgcc tgcagttctc ctacgccgcc gcgtggcgta acagtagtga ataatttcga
     6421 cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc gttttgaacg
     6481 tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc tgaatgtcat
     6541 taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga aagcgtactt
     6601 taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt tctatggcgg
     6661 ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg gcccggaccg
     6721 cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga aacaggagat
     6781 gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg taca
//
LOCUS       DAZGXZ010000502         3526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.guided.261, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000502 DAZGXZ010000000
VERSION     DAZGXZ010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.guided.261"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            complement(<1..391)
                     /locus_tag="ACHKJF_005758"
     CDS             complement(<1..391)
                     /locus_tag="ACHKJF_005758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0952882.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADE"
     gene            652..1110
                     /gene="gpt"
                     /locus_tag="ACHKJF_005759"
     CDS             652..1110
                     /gene="gpt"
                     /locus_tag="ACHKJF_005759"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0952883.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1202..2446
                     /gene="frsA"
                     /locus_tag="ACHKJF_005760"
     CDS             1202..2446
                     /gene="frsA"
                     /locus_tag="ACHKJF_005760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0952884.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            2504..2905
                     /gene="crl"
                     /locus_tag="ACHKJF_005761"
     CDS             2504..2905
                     /gene="crl"
                     /locus_tag="ACHKJF_005761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0952885.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(2944..>3526)
                     /locus_tag="ACHKJF_005762"
     CDS             complement(2944..>3526)
                     /locus_tag="ACHKJF_005762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0952886.1"
                     /translation="FFGVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDF
                     AISGAYTNSDRTNEQNLQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGG
                     FANKTQNFEAVAQYQFDFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNM
                     SAFVDYKINQLDSDNKLNINNDDIVAVGMTYQF"
BASE COUNT          865 a          840 c          902 g          919 t
ORIGIN      
        1 tttcgtcagc cagaactgcc agtgcagagg ccataccaat gccgttatcc gcacccagcg
       61 tggtgccgcg cgctttaacc cattcgccat caatataagg ctggatagga tctttcgtga
      121 agtcatgcac ggtgtcgtta tttttctgcg gcaccatatc gaggtgggcc tgtaagacga
      181 ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata ttacctacct
      241 gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat tcagcgagtt
      301 gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata tcccacagcg
      361 gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat cacccgcaaa
      421 acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca ggcttgcgag
      481 atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
      541 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
      601 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
      661 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
      721 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggt
      781 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
      841 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
      901 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg
      961 tatccaaaag cgcactttgt caccatcttt gcaaaaccgg ctggtcgtcc gctggttgat
     1021 gattatgttg ttgatatccc gcaagatacc tggatcgaac agccgtggga tatgggcgtc
     1081 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact gccgggcgtt
     1141 gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg aggctgcatc
     1201 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     1261 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga
     1321 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     1381 tattgaccca cgtgaaatcc tagacgtcca ggcacgtatt gtgatgagcg atgctgaacg
     1441 taccgacgat gatttatacg atactgtgat tggctaccgt ggtggcaact ggatttatga
     1501 gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt gcggaagaag atccgcaact
     1561 cagtggtcga cactggctgc atgcggctac gttgtacaac attgccgcct atcctcatct
     1621 gaaaggagat gacctggcag agcaagcgca ggctttatca aaccgcgcct atgaagaggc
     1681 cgcccagcgt ctgccgggca cgatgcggca gatggagttt actgtacccg gcggtgcgcc
     1741 catcaccggc tttttgcata tgccgaaagg cgatggcccg ttcccgacag tattaatgtg
     1801 tggtggtctg gatgcgatgc agacggacta ttacagcctg tatgaacgtt attttgcgcc
     1861 gcgcggcatt gcgatgctga ctattgatat gccgtcggtg ggcttttctt caaaatggaa
     1921 gctcacccag gactccagcc tgttgcatca gcacgtctta aaggcgctgc ctaacgtacc
     1981 gtgggtggat cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta acgttgccgt
     2041 gcgtctggca taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg gtccggtagt
     2101 tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt atcttgacgt
     2161 tctggcgagt cgtttgggga tgcatgatgc ttccgacgaa gcgttgcgcg tggagttgaa
     2221 tcgctattca ttaaaagtgc aaggattgct gggacgtcgc tgcccaacgc caatgttatc
     2281 aggctactgg aagaacgatc cgttcagccc ggaagaggac tcacgcttaa tcacctcatc
     2341 atctgctgac ggtaaattat tagagatccc atttaacccg gtgtatcgga attttgacaa
     2401 aggtcttcag gaaatcaccg actggatcga aaaacgtttg tgttaaaaat ttgctaaatt
     2461 ttaccaattt ggtaaaacag ttgcatcaca acaggagatt gcaatgacgt taccgagtgg
     2521 acacccgaag agcagattga tcaaaaaatt taccgcacta ggcccgtata ttcgtgaagg
     2581 taagtgcgaa gataatcgat tctttttcga ttgtctggct gtatgcgtca acgtgaaacc
     2641 ggcaccggaa gtgcgtgagt tctggggctg gtggatggag ctggaagcgc aggaatcccg
     2701 ttttacctac agctaccagt ttggtctgtt cgataaagca ggcgactgga agagtgttcc
     2761 ggtaaaagat actgaagtgg ttgaacgact ggagcacacc ctgcgtgagt ttcacgagaa
     2821 gctgcgtgaa ctgctgacga cgctgaatct gaagctggaa ccggcggatg attttcgtga
     2881 cgagccggtg aagttaacgg cgtgagtgaa atgtgccgga tgcatcacat ccggcaatat
     2941 ctattaaaac tgataggtca tgccaaccgc gacaatatca tcattattaa tattcaattt
     3001 gttatcgcta tccagttggt tgattttata atcaacaaac gctgacatat ttttgttgaa
     3061 ataatatgta gccccgacat cgatataatt gaccagatct tcatcaccga taccttcaat
     3121 atctttccct ttcgataaga cataacccag cgatggacgc agaccaaagt caaactggta
     3181 ttgagcgacc gcttcaaagt tctgtgtctt attggcaaag ccgccagtta ttggcgtcat
     3241 tttgcgtgtt tcagaataaa aagttgccag ataaatatta ttggcatcgt atttcagacc
     3301 tgtagcccat gcttctgcac gcttgcctgt gccacggctt tgcaggttct gctcgttggt
     3361 gcgatctgag ttggtatagg ccccactaat ggcgaaatcg ctgccgccaa agtcatatgt
     3421 caatgacgtg ccgaagccat cgccgttttg ctttttaacg tcgcggtttt cgtttttccc
     3481 ttgatattgc agggttaagt tcaggccatc gataacgccg aagaag
//
LOCUS       DAZGXZ010000503         1127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.guided.365, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000503 DAZGXZ010000000
VERSION     DAZGXZ010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.guided.365"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHKJF_005763"
     CDS             130..1089
                     /gene="stxA2b"
                     /locus_tag="ACHKJF_005763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit A"
                     /protein_id="HFP0952887.1"
                     /translation="MKCILLKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRT
                     EISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAG
                     FVNTATNTFYRFSDFAHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSS
                     YLALMEFSGNAMTRDASRAVLRFVTVTAEALRFRQIQREFRLALSETAPVYTMTPEEV
                     DLTLNWGRISNVLPEFRGEGGVRVGRISFNNISAILGTVAVILNCHHQGARSVRSVNE
                     EIQPECQITGDRPVIRLNNTLWESNTAAAFLNRRAHSLNTSGE"
BASE COUNT          302 a          239 c          266 g          320 t
ORIGIN      
        1 gcaatcggtc actggttcga atccagtaca acgcgccata attatttacc aggctcgctt
       61 ttgcgggcct tttttatatc tgcgccgggt ctggtatcgt attacttcag ccaaaaggaa
      121 tacctgtata tgaagtgtat attgttaaaa tgggtactgt gcctgttact gggcttttct
      181 tcggtatcct attcccggga atttacgata gacttttcga ctcaacaaag ttatgtctct
      241 tcgttaaata gtatacggac agaaatatcg accccacttg aacatatatc tcaggggacc
      301 acatcggtat ctgttattaa ccacacccca ccgggcagtt attttgctgt ggatatacga
      361 gggcttgatg tctatcaggc gcgttttgac catcttcgtc tgattattga gcaaaataat
      421 ttatatgtgg ccggattcgt taatacggca acaaatactt tctacagatt ttcagatttt
      481 gcacatatat cagtgcccgg tgtgacaact gtttccatga caacggacag cagttatacc
      541 actctgcaac gtgtcgcagc gctggaacgt tccggaatgc aaatcagtcg tcactcactg
      601 gtttcatcat atctggcgtt aatggagttt agtggaaatg ccatgaccag agatgcatcc
      661 agagcagttc tgcgttttgt cactgtcaca gcagaagcct tacggttcag gcaaatacag
      721 agagaatttc gtctggcact gtctgaaact gctcctgttt atacgatgac accggaagaa
      781 gtggacctca cactgaactg ggggagaatc agcaatgtgc ttccggagtt tcggggagag
      841 ggtggtgtca gagtggggcg aatatccttt aataatatat cagcgatact gggcacagtg
      901 gcggttatac tgaattgcca tcatcagggg gcacgttccg ttcgctccgt gaatgaagag
      961 atacaaccag aatgtcagat aactggcgac aggccagtta taaggctaaa caatacttta
     1021 tgggaaagta ataccgcagc tgcttttctg aatcgcaggg ctcactcttt aaatacatcc
     1081 ggagaataac gggagttaaa tatgaagaag atatttgtag cggcttt
//
LOCUS       DAZGXZ010000504          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2103H69960
            SAMN43267427-rid23070293.guided.472, whole genome shotgun sequence.
ACCESSION   DAZGXZ010000504 DAZGXZ010000000
VERSION     DAZGXZ010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267427
            Sequence Read Archive: SRR30307197
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350515.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350515.1
            Genome Coverage       :: 80x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:42:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,764
            CDSs (total)                      :: 5,673
            Genes (coding)                    :: 5,458
            CDSs (with protein)               :: 5,458
            Genes (RNA)                       :: 91
            rRNAs                             :: 2, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 4 (5S, 16S, 23S)
            tRNAs                             :: 77
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 93 of 215
            Pseudo Genes (incomplete)         :: 112 of 215
            Pseudo Genes (internal stop)      :: 54 of 215
            Pseudo Genes (multiple problems)  :: 37 of 215
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267427-rid23070293.guided.472"
                     /serotype="O128:H 2"
                     /isolate="2103H69960"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-03"
                     /collected_by="SSI"
                     /note="subtype: stx2b"
     gene            15..278
                     /gene="stxB2b"
                     /locus_tag="ACHKJF_005764"
     CDS             15..278
                     /gene="stxB2b"
                     /locus_tag="ACHKJF_005764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx2b subunit B"
                     /protein_id="HFP0952888.1"
                     /translation="MKKIFVAALFAFVSVNAMAADCAKGKIEFSKYNENDTFTVKVAG
                     KEYWTNRWNLQPLLQSAQLTGMTVTIKSNTCASGSGFAEVQFN"
BASE COUNT          115 a           61 c           92 g          108 t
ORIGIN      
        1 aacgggagtt aaatatgaag aagatatttg tagcggcttt atttgctttt gtttctgtta
       61 atgcaatggc agctgattgt gcaaaaggta aaattgagtt ctctaagtat aatgagaatg
      121 atacattcac agtaaaagtg gccgggaaag agtactggac taaccgctgg aatctgcaac
      181 cgctactgca aagcgcacag ttaacaggaa tgacggtaac aatcaaatca aatacctgtg
      241 cgtcaggttc aggatttgct gaagtgcagt ttaattaata tcagaagcat tgctggtttc
      301 gtagtgtgca gcaatgtagt tacagtgtaa tcaatgtcac aattcagtca gttgacggtt
      361 gcctgtctgg ctgagc
//
